LOCUS       DAOPBQ010000001        28251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_1831_length_28251_cov_4.714357, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000001 DAOPBQ010000000
VERSION     DAOPBQ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 28251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28251
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1831_length_28251_cov_4.714357"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="PKW60_00005"
     CDS             complement(<1..1152)
                     /locus_tag="PKW60_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPJ97838.1"
                     /translation="MAAAAAPDVFFITVDTLRADHLGMYGYERPTSPHLDALAETSLV
                     FDDCVCEVPLTSPSFGSMLSSRYPRSTATPRNGLPMPASVPLVTERFRDAGYQTICIQ
                     SNWTLKRDLSGLDRGFEQYDDDMKAKRWGVLKAERSGDEVTRRALAALARRDPARPLF
                     CWLHYSDPHAPYRFHDRCHPWGVPLRKLDEVSQTRARYDSEVAFTDRQIQQVLKALPK
                     ENAYILFIADHGESLHEHGYVGHGRRVYQPGVRIPLFIYGPGIAPGRTAFPARAIDIG
                     PTLLGLAGLELFPGMEGVDLLRNKESGPRVRVFETYGGAVPNLPGVAAFMGSQPPIRQ
                     AVLDGVWKLIVGGPRCELFHLGNDPHELTNLHDDRPEEAARLRALLDECD"
     gene            complement(1180..1668)
                     /locus_tag="PKW60_00010"
     CDS             complement(1180..1668)
                     /locus_tag="PKW60_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97839.1"
                     /translation="MKRIGCVLVLTLLIATGLGCDAQRRQPRLSSATITPARLQPGDS
                     AVITVAVERDRFSIVDKVCAVVQEDRRMRFDLKDDGNAPDAEARDGIWSLMVDVPFMA
                     PPGSFTLEFTAYDVKGNVISVSGPQGGGMPLSTTCVFSVEHSLAETPEADTTETQPAE
                     NN"
     gene            1922..2731
                     /gene="truA"
                     /locus_tag="PKW60_00015"
     CDS             1922..2731
                     /gene="truA"
                     /locus_tag="PKW60_00015"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPJ97840.1"
                     /translation="MNTHEETVSAPPPAYTTLKAVVRYDGAPYAGWQVQPNETTVQGC
                     IETALSRLANGPVRIHGASRTDAGVHAFGQVFSWQWPREASLGRLQRSLCKMLGPSLR
                     IDSIEHAPGGFHARKSAHSKRYAYVLHLTRHPDPFLRRYAWAIPWELDLALLDSLATR
                     ITGTHDFAGFQGGGSSCCQTVRTLFSVARRPGPIAGPQGLPGVWHLELHGDGFLYKMV
                     RNLAGTLVHIARGNLPPEHLETLLHRPGPYHGYTAPPHGLFLMEVLYPDFK"
     gene            3295..4143
                     /gene="rfbD"
                     /locus_tag="PKW60_00020"
     CDS             3295..4143
                     /gene="rfbD"
                     /locus_tag="PKW60_00020"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPJ97841.1"
                     /translation="MKVLVVGAKGQLGRELCLLFGADADVTGVDLPQLDITDHDSVET
                     LVADTAPGIIINAAAYTDVEGAEDNRELAFAVNETGARIIAGAARRAGVPVVYYSTDY
                     VFFGAQDDALEPSDPIMPRGVYAESKAAGEEATRNANPDHFIIRTAWLYGPGGNNFVE
                     KILRAAATRPAVKVVQDEVGSPTHTFDVAEATRALCATTAYGTYHAVNSGQCSRFEFT
                     REIFRLAGVGTPLCPCTADEFPMKAPRPSYSVLCTRKLTRACGYVMRPWQQALAHYFQ
                     RRKEAV"
     gene            4140..5141
                     /gene="rfbB"
                     /locus_tag="PKW60_00025"
     CDS             4140..5141
                     /gene="rfbB"
                     /locus_tag="PKW60_00025"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPJ97842.1"
                     /translation="MTRIVVTGGAGFIGSNFVRNMLAAYPELHIVNFDKLTYAGNLDN
                     LKDLDPARHTFVQGDIADKAALLDAMTGCDAIVNFAAGSHVDRSIMGADDFIDTNVTG
                     VYNICEAARELGTPRVLLVSTDEIYGSIDEGSFTEEDPPHPGNPYSASKAGGELIALA
                     HFNTFGTPIMITRGSNTYGPYQYPEKILPLFVTNAIDEQPLPLYSGGEDNVRDWLYVD
                     DHCRGIDLALRKGLPGQVYNVAGGNERRNIVLTRKILDLLGKSEDLIKLVKDRPGHDR
                     RYSINAEKLSALGWAPRMDWDEGMALTVQWYRDNEWWWRKIKTGEFLEYYRKQYHER"
     gene            complement(5166..6860)
                     /gene="argS"
                     /locus_tag="PKW60_00030"
     CDS             complement(5166..6860)
                     /gene="argS"
                     /locus_tag="PKW60_00030"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPJ97843.1"
                     /translation="MDLFNSIAESITKAFPEIERADLAFAPTPRPDLGDVALRIFDAA
                     RRLQCPPPQLAARIAGEVLFGPEVVEASPAGPYVNFKLNRELYARRIVASVLDAGDSF
                     GTNRTGAGQRVLIEHTSINPNASPHVGRARNALIGDSVVRLLRFEGYEVEVHYYVNDM
                     GRQIGLLVLECPEPEQLSFDQVLDIYVKANQRAEAEPEFAAQGYELLVKMEEKDPDTV
                     EKFHRITELCLRGQLAVLERIGARYDVFDRESDYLRDERLEQVLAALREKDAVFTDED
                     ERVVVDLAKLGHERDEGRYFVLMRANGSSMYGCRDLSYTIHKMGLGADLNLIVLGEDH
                     KLYAEQVALILGAAGFTAPEPIYYAYITLKEGKMSTRRGTVVLLSAFLDEAESRAAEK
                     VEEQCRDLSPEERSAIIKQVALAAVRFAILRVNPNKNVVFEWESALSFTGDTGPYVQY
                     SCARIASILRKFGDCPETVDETFPIDTAVEWALLMQLASFPSVVAGAVVQRNCAPIAQ
                     YALDTARAFTTFYHECPVIAAPTREQQVARAQLCRATRQTIQNALGLLGIAAPDRM"
     gene            complement(7113..9041)
                     /locus_tag="PKW60_00035"
     CDS             complement(7113..9041)
                     /locus_tag="PKW60_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPJ97844.1"
                     /translation="MLLAMMALAAGDPWQWPLTLPPAVTSSFAEYRSGRFHAGLDLRT
                     GTATPEVYAAADGYISRIRCSPWGYGKAVYLQTSDGHTIVYAHLAEFTNGLRDYVRQA
                     QHAREKYAVDLQPEPGAFPVRRGDLLAFAGRTGTRAPHLHWEFRDAQSRPLNPRLLGI
                     TWPDTTRPHIRKVLLVPVEPGATLNGDLVPVVLEVRPDGNGGGHTCAAVTARGKIGIA
                     VDVIDPANGGANVLGVHTLRTVVDAQETFRVQNDVLSYETMGDGSVSWHPYYRSEGPF
                     LLQWRWEGNQSPNFSVSEADGRIDAGGKPLDVLIEACDYFGHTATLPLRIEPEAAEPP
                     APPSRGSNAQGTLGIDCFDQWLLLTARFPDDEPETPLLQSTGAEPEPAFRRVGAGLFR
                     AAIRAAQHSDQVALAVEHPRLAPYMETIEVFQRGRGRIACFGDLVVRANARSVYGTLG
                     LRMSEETVTGTSGLVAMGKGYRLWPTDAPLDVPLEISFPESSGAADAANIAIYRKSGN
                     GWARQDTLRGGGRLTIHTGSLGVFAPMADPVAPTITEVVPAAGTGPTTNRRPRIQACV
                     TDNASGVETVRITANGAWLLAEFDPFASPAATVTWQQDEDLTLGENVIVIQATDAAGN
                     TAEVTRTIHIVETLPSPS"
     gene            complement(9044..10183)
                     /locus_tag="PKW60_00040"
     CDS             complement(9044..10183)
                     /locus_tag="PKW60_00040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPJ97845.1"
                     /translation="MKVIRIAMAVIWATGVSLAAWSQAPNLEGLDLVLDVVPAGPVAL
                     VHNRPIPSEAFVELYKSELNRYQQLRPDDTISDAFRVGLALRCMRILIEKEVLLQEAE
                     KRNLTLTDEYVQEQWAKEVEMLGQQLSGGQAEPLSEDEVLKRAGATREEALAELRNAL
                     LIEKIRGIIAKEKGVEVTDAEVKKQFEQMRKDNARQPDRAHLRQIFVRAPRQNSNGAA
                     TQRQEARKKVEEALSRIQSGRKFDAVAKEMSDEPFRSKGGDLGTARIDTMPDFMAEAA
                     RTLKPGEMSGIIESEFGYHIIELLELLPGEEITFEKAAPYIKNTLLLRKTNLAVEEFC
                     REATSHEDAIQTALNLDRKLVTHPELLDMLDPGEMPEEIGMSPKP"
     gene            complement(10186..10527)
                     /locus_tag="PKW60_00045"
     CDS             complement(10186..10527)
                     /locus_tag="PKW60_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97846.1"
                     /translation="MPRTIVRRRENFRVILPDSDKLGLLVASFCATLVMAIHFIFSSA
                     EPLEIAYRVVLTFAASWTATAALVHYLISAMMAEARAERAARRRTRAEAERAMQDEAD
                     DESAPEGMTGE"
     gene            complement(10647..13847)
                     /gene="carB"
                     /locus_tag="PKW60_00050"
     CDS             complement(10647..13847)
                     /gene="carB"
                     /locus_tag="PKW60_00050"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPJ97847.1"
                     /translation="MPRRDDIRKVLIIGSGPIVIGQACEFDYSGTQACKALRKLGYEV
                     VLVNSNPATIMTDPGMADQTYIEPLNFETLREIIAKERPDALLPNLGGQSGLNLASEL
                     ARGGVLEEFGVKVIGVQVDAIERGEDRSAFKKTMSQLGIETPRSELAYSIEDAERIAE
                     KLGYPVVLRPAYTMGGTGGGLVYNVEELRTITARGLSASLVGQVLVEESVLGWEELEL
                     EVVRDANNHMITVCFIENVDAMGVHTGDSYCTAPMLTISPELQDRLQKYSYAIVEAIE
                     VIGGTNIQFAHDPETGRVVVIEINPRTSRSSALASKATGFPIAMVSSMLAAGVTLDEI
                     PYWRDGTLDRYTPASDYVVVKFARWGFEKFRGIEDKLGTQMRAVGEVMSIGKNYKEAF
                     QKAIRSLESGRYGLGFARDFNQKSLDELMVLLKHPTSERQFVMYEALRKGARIEELHR
                     LTHIKPWFIEQMKELVDLEEEILACKGKELPDALLARAKKDGFADRYLARLLGLPEDR
                     VRQQRLRTGVTEAWDAVPVSGVEDAAYYYSTYNAPDKVKVSPNKKVMVLGGGPNRIGQ
                     GIEFDYCCVHAAFALREQGYESIMVNCNPETVSTDYDTSDKLYFEPLTVEDVLSIYEK
                     ERPEGVIVQFGGQTPLNIARELASAGVPILGTTPETIDLAEDRDRFRQTMDRLNIPQA
                     QSGMASTLEEALAIAAEIKYPLMVRPSYVLGGRGMEVVHDEEMLRHYVAAAVDVSPER
                     PILIDKFLENAVEVEADAISDGTDAFVPAVMEHIELAGVHSGDSACVIPPISIPAKHI
                     ETITEYTRKIAVEMNVVGLMNIQYAIANDTVYILEANPRASRTVPLVSKVCGISMANI
                     ATQVMLGKSLADMNLRPRHFPHFGVKEAVFPFNMFPEVDPVLGPEMRSTGEVLGLADS
                     FGLAFFRAQEATQLKLRLEGAVLITVAEPDKPGVLEAARQFQNLGFRIRATLGTQAFL
                     SEHGVKSELIKKVHEGRPNIVDAITNGEIQLVVNTPAGKLSVTDDSYIRKSAIKYKVA
                     YVTTTAAAIAAARGIAARKQDTATVRSLQEYHKRIS"
     gene            14335..16935
                     /locus_tag="PKW60_00055"
     CDS             14335..16935
                     /locus_tag="PKW60_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HPJ97848.1"
                     /translation="MKRFSFNTFLEADGNRAALRICKRIAELAPVSPMPVLLLGEEGC
                     GKTHLLYSIWKRVKATSPRTSLAVVTAREFRQEVLALIDDPSPVEKAESAILLIDQLE
                     LFDVLLDELESVVRIFINNNRYVLLASNVHPRRLKVLPPGLRDLIAAGQTVDIRLREG
                     ETQLEVLRRELRRESEAVIEKQQVEIDRLRGLLERVGASAGGADASAAVREALEQARA
                     RNAELTRQLDTATRLGLGLQEELESVKSRLGAAERGETPVKAADSEESAAGRAERDEA
                     REELEALRRELARRGDTEEEIEQLRAALAAAQAEKERALAETAAEQETERQRLEAEVA
                     ALAQELVVARNDSAQARHEANMLVERAERLVSQIEGTQLRFRETEQKQRRQIEELETL
                     LAMQGPAEMQDERLAALQEQVEASEGRVYALQQDFERERRELIERLEQDEQRIQDELS
                     RALEAARAAHEAHEALSVEHAQLRDALKSAKDECESFRTQLDALEQERETLRVQLENL
                     EQERESFHTQLENFEQERESFRTQLSDLEQEREKLRVQVENFAQGDESLRAQHDNLAQ
                     EREALRAQLEHLEREHESLRAQLENLEQEHESLGSDLNASRAERAAAESALEELRQTR
                     ENLESELNGSRAEQDRLREELEQAHRENQRRQSALDESRVQLETAQSQARQLEARLSE
                     QAADMEALRHDAAAQVARANAQAGELEGQVAQLRAQLVQAGESRREAARRIEEMRANL
                     AASAHVLGEWSSQLASHEAPPEASGEELDEASWAPYQDSPKARPAAAGSAQESQTLPE
                     IDEDDQFDGDRMVLPPAPDEEDVEPGAGYGSDPGAGAPVWTDSEPPANFDVPSPPLEP
                     LQDLSPLEDE"
     gene            17045..17614
                     /locus_tag="PKW60_00060"
     CDS             17045..17614
                     /locus_tag="PKW60_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPJ97849.1"
                     /translation="MDTLESRIIRLLTEIHPLDRVPRAGYVLRGVPEPESVAAHSHFV
                     ALLALLICDACPGQFDRGKALTMAIMHDLAEARLMDIPMTVSDAYLKGAKRDAEQAIF
                     EDLVAGFPERYAQYHRELLDAVSPEARLIRGLDKAQMMLKIVMYQKEGRGRLDEFWNN
                     PRNFADFGVEPVSRLFDAICAHAGRCRPK"
     gene            18074..20257
                     /locus_tag="PKW60_00065"
     CDS             18074..20257
                     /locus_tag="PKW60_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97850.1"
                     /translation="MEKIGAVVRALGNLRRAAHRLKLRDLYYCAEEQERRAREALEGG
                     LLPETTIDSSLEMIDVLKRYLAGVEESLRTGEPLPSPRMLPSLRRSAESRRVPRRIAR
                     TSTPRDARKRLGELLMESGDISEGELQRALFELEEAGPPRLIGELLLEELQISREQLD
                     RALAGQQDASQSLRLGEILVSLGAISPQGLVRGLEKQRHPKAPRLGERLVRTGRVPAK
                     RVVLALRCQGMIRDSVRHGLATLAARFATRVTEMGPPQQRERLSADERAALSRFRDEA
                     RNLLNAADRGLLALESESTDPHALRSVRRSIYALSRFARYLGLADIGHFARAFSGYLA
                     RAAGNAFSLEGPRLDVAFDCIDVLRRHVDYVDDALMTQGRIRRERQLPEYIALLRSLS
                     VGWQKDLRVSRLQPSEQGQRLGDILMASGGVTREALDAALAAQARTQESARLGELLVD
                     EALISRDQVNAILAAQQQDVSIGRLGDVLVQWGLVDRADIDAAIARRQAQGRPRLGEL
                     LVRMGAVRAKAAAHALRRQRARLAGAAAAVLVASSILAPASPAAASPALATGDSAIAR
                     ALDQGLAAGAERDRLGGAVEAALVTRPLALDIERDGMLDGWEVWNALHPGRSAGMAGA
                     ARRNSLTSGIEIAPASQQEPEASSEAEAWDYIEWQRIADPASAERHPMPQARGELSFS
                     DLQYVINAALGLDTPVPPKLNRAGAADAPDILGIIHAALGIAPQA"
     gene            complement(20279..20695)
                     /locus_tag="PKW60_00070"
     CDS             complement(20279..20695)
                     /locus_tag="PKW60_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPJ97851.1"
                     /translation="MKSYRKELWFSATQRRQIIHITPQIEECLRESGVKEGLVLVNAM
                     HITASVFINDNESGLHADFERWLEKLAPEKPYDQYAHNGYEDNADAHLKRTVMGREVV
                     VAVTDGKLDFGPWEQIFYYELDGKRRKRVLVKIIGE"
     gene            20899..22305
                     /locus_tag="PKW60_00075"
     CDS             20899..22305
                     /locus_tag="PKW60_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ97852.1"
                     /translation="MKHQNLTRRAFIATSTLAASQLGCATAQTRIAMNDAQVVPGKIS
                     PNERLNVAAIGAGGKGFSDIMSCKGENIVALCDVDWKRCEKAFNELPDARRFRDYRNM
                     LEQMPEIDAVTISAPDHIHAPAAILAMKMGKHVYVQKPLTHTVQEARWMTLVAREAGV
                     ATQMGNQGHSGNGVRELCEMIWSGAIGQVREAHVWTHRPVWANQGRPDPLPSAVAPEH
                     LDWDRWIGPAPMRPYAEGYAPHDWRAWLDFGGGSLGDMACHIMDPVYMSLKLVEAASY
                     TVEVVMQEGRNEETFPLTTTIKYSFPARGDMAPVDVYWYDGHWEDPKTGEKMYNRPPR
                     PKGIPEDQELGDDNKNGSYLVGDKGIMTQGEYGGRPRLMPDEKMADYTKPEPFLERIP
                     DQNHYQDWLQACKGGKPACSNFDYAGPFTEMVQFGNLVVKSGKKLHWDNKKGVVTNVK
                     NAEAVVSKEYRKGWELPI"
     gene            22376..23047
                     /locus_tag="PKW60_00080"
     CDS             22376..23047
                     /locus_tag="PKW60_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HPJ97853.1"
                     /translation="MAARAECEGAGIARVPKYGVLRPGQHDSETLAALLSRVRACREC
                     AAELPLGPRPVLCASETARILIVGQAPGTRVHATGIPWNDPSGDRLRDWMSVDRDTFY
                     DVSRIAIIPAGLCYPGRYDRGGDLPPRKECAERWLEPLLRHLPNVALTLLCGHHAQAL
                     HLGVRAKESLTETVESWREYFPRYLPLPHPSFRNLLWMKKHPWFERELLPVLRKRVRA
                     LLRKR"
     gene            23172..24968
                     /locus_tag="PKW60_00085"
     CDS             23172..24968
                     /locus_tag="PKW60_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPJ97854.1"
                     /translation="MGWEAWFSFGIIGAILFGLATNRASDALLVGGVVLMGLAGILSP
                     SEMFAGFSNTGMLTVGALYIVTAGLRETGALQSVGDWVLGGARNEHHVLRRLSWVTAM
                     SAFLNNTPIVAMFIPIVEDWCRKHQVSPSRLLIPVSYFSILGGTCTLIGTSTNLVVNG
                     LMIQAAKNDPSLADRLFEMSIFELGRVGLPYAFVGTAYLLLAGPRLLPQRQGARDRLG
                     AAAREYLANMRIETGCRLAGKRVEEAGLRRLTGLFLIDIWRNNRMIAPVGPDEVLEEG
                     DILTFTGVVDHIVDLERIPGLVAFVDEDYEAQAAARRHEMLCEAVVSRTSPLINKTIR
                     EANFRARYNAAVVAVHRGGHRLQGRVGDIELQQGDTLLLQAGPHFVDAQRNNSDFFLV
                     SGVSNSCALRDDKAWLSLGLLGLLVLLMTTGKMEIVLAAFLVAGLMIATRCVSVADAR
                     RSVDWQTLITIGAAFGLGTGLVNSGAVEAISGRVVAAMGPWGPRAVLLGVYLMTTLFT
                     EIITNNAAAALMFPFAVAIAQGLGVDPRPFAMTIAFAASASFMTPLGYQTNLMVYGPG
                     GYSLRDFVRIGLPLNVLLMLTATLLVPLAWPF"
     gene            complement(24992..25876)
                     /locus_tag="PKW60_00090"
     CDS             complement(24992..25876)
                     /locus_tag="PKW60_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPJ97855.1"
                     /translation="MNHRADPPRSADYPAAYPARMPGAVVRATFRLARLLAASACCYA
                     ALLAVDTLAWLSPARFEPHRRPIALAWIRQLRKGIGQRVTTYGPVPRGQLFFVSNHVT
                     WQDFLIQDLFPQGRLVAIDSLRVLPVLGRFFRALDYVFVSRHPEEVPRVNAEIRRRLA
                     RGQSIMLAPESVISPGRSIQRFRAALLGSAAQDRFPVHYGAIHYHTPWGWPPPSQSVL
                     FGPDPYYRTPEGEIPPAQLEMWGPPRPFLPHLLRLLSLPYHRLTLTLGKDPIIRDDRI
                     VLANALHDAVSELFHPID"
     gene            complement(25873..26343)
                     /locus_tag="PKW60_00095"
     CDS             complement(25873..26343)
                     /locus_tag="PKW60_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPJ97856.1"
                     /translation="MLTQTSETALRVLVFLLLREARAPVPPARIAQHINASPSYTAKV
                     TTALVKADILRAHRGMKGGVTLSRPPEAITLLDVVEACQGKILGDFCQDFDRMELVCA
                     FHRAMAELHEAIITILKRWTLADLAQKPFPSEEIRQTIQCTMELTLADDEDAAE"
     gene            complement(26523..27824)
                     /gene="hcp"
                     /locus_tag="PKW60_00100"
                     /gene_synonym="priS"
     CDS             complement(26523..27824)
                     /gene="hcp"
                     /locus_tag="PKW60_00100"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006973247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPJ97857.1"
                     /translation="MYCDQCEQTRDGIACTDTGACGKNADVESLQKILLYGLKGMCSY
                     KHHARRLGKTDPVVDAFVEDALFATVTNVNFDMDSLLELVLQCGEMNLRTMQLLNDGH
                     VDTFGKPSPVDIQEGIQDGPGILITGHDMVDLLDLLEQTKGTRIKVYTHGEMIPAHMY
                     PKLREYPHLAGHYGGAWQKQRMEFEAFGGPILATTNCVLIPNPAINTYLDRLYTTRVT
                     AVPGAKRITTNDFSEVIKRALEIGPLTPTELVTRTVGFHYSVILDLADKIIDAVKSGT
                     IKRFFVIGGCDGADPGRNYFRDFAYNAPEETIILTMGCGKYRIREHEYGTVAGLPRLL
                     DMGQCNDSYGAIQVALALAKAFECGVNDLPLTLLISWFEQKAVAVLLTLLHLGVKGIR
                     LGPTLPAFVSPNVLALLQEKFDLQPIGKDAMADLNTVMANA"
BASE COUNT         5281 a         8866 c         8809 g         5295 t
ORIGIN      
        1 gtcacattcg tccaagagcg cgcgcaggcg ggccgcctcc tccggccggt cgtcgtggag
       61 gttcgtcagc tcgtgcgggt cgtttccaag atgaaataat tcgcagcgcg gcccgcccac
      121 gatgagtttc cataccccat cgagcaccgc ctgccgtatc ggaggctggc tgcccatgaa
      181 agccgcgact ccgggcagat tcggaacggc gccgccatac gtttcgaata cccgcacgcg
      241 aggtccggac tctttattcc ggagcaggtc cactccctcc attcccggaa acagctccag
      301 tcctgcgagg cccaacaacg tcgggccgat gtctatcgcc cgcgcaggaa atgccgtcct
      361 gccgggcgcg atgcccgggc cgtagatgaa cagcgggatg cgaactccgg gctgatacac
      421 gcgccggccg tgacccacat agccgtgttc gtgcagactt tccccgtgat ccgcgatgaa
      481 gaggatatac gcgttctctt ttggaagggc cttcaacacc tgctgaatct ggcgatcggt
      541 aaatgccacc tctgaatcgt accgcgcgcg ggtctgggat acctcatcca gcttgcgcag
      601 cgggacaccc cacgggtgac accggtcatg aaaccggtac ggggcatgcg ggtcagaata
      661 gtgaagccag cagaacaacg gccgggccgg gtcgcgccgg gcaagtgcgg caagcgcacg
      721 gcgggtgacc tcatctccgg accgctccgc tttcagcacc ccccaccgct tggccttcat
      781 atcgtcgtcg tactgctcga acccgcgatc gaggcccgaa aggtcccgtt tcagcgtcca
      841 gttgctttga atgcagatcg tctggtagcc cgcgtcgcga aaccgctccg tcaccagtgg
      901 caccgatgcg ggcatgggca atccgtttcg cggcgtcgcc gtgcttcgcg gatagcgcga
      961 cgacaacatg gacccgaacg acggcgacgt cagcggaact tcgcacacac agtcgtcaaa
     1021 caccagcgat gtttccgcga gggcgtcgag atggggggag gtaggccgtt cgtagccata
     1081 catgcccagg tgatcggcac gcagggtgtc caccgtgata aaaaacacgt ccggcgccgc
     1141 tgccgcggcc ataaacatca atatcccgac catgggcagc taattgttct cagcgggctg
     1201 ggtctccgtt gtgtccgctt ccggcgtttc cgccagagaa tgctccacgc taaatacgca
     1261 cgttgtggac aaaggcattc cgccgccttg cggccctgac accgaaatga cattgccttt
     1321 cacgtcgtag gccgtgaact ccagcgtgaa ggacccgggg ggcgccatga acggcacatc
     1381 caccatcagc gaccagatcc catcccgcgc ttccgcgtcc ggcgcgttcc cgtcgtcttt
     1441 gaggtcaaac cgcatgcgcc ggtcttcctg aaccaccgcg catactttat cgacaatgga
     1501 aaagcggtcc cgttcaacgg ccacggtaat gacggccgaa tctcccggct gcagccgggc
     1561 cggtgtgatc gtcgccgaag acagacgcgg ctggcgcctc tgggcgtcgc atcccaggcc
     1621 cgttgcaatc aacagagtca gcacaaggac gcatccaatt cgcttcattg cctcttctcc
     1681 agcactcttt tgaaatctgg cgcgggcgcg cactcttgcc ggtgcagccg gcagctgtac
     1741 tccagactag cgaagccgcg cgaagtctgt caaacaaatg atcgaaacca acgcggcaca
     1801 aacgtgactg ccgctgacga tgaggcagaa ccgcgggttc cgcgcccggc gctcgggcgg
     1861 gcagctcaca cgggctcggc acaattgcgt attgcgcctc cagaatccat aattcgagcc
     1921 tatgaacact cacgaagaga cggtctcggc gcccccgccc gcgtacacga ccctcaaggc
     1981 cgtggtccgg tacgatggcg cgccgtatgc cggctggcag gtgcagccga acgaaaccac
     2041 ggtgcagggc tgcatcgaaa cggcactctc gcggctcgcc aacgggcccg tgcgcatcca
     2101 cggcgcctcg cgcaccgatg ccggcgtaca cgctttcggt caggttttct cctggcaatg
     2161 gccccgggaa gcctcgcttg gcaggctcca gcggtccttg tgcaaaatgc tcggaccgag
     2221 ccttcgcatc gactccattg aacatgcgcc cggcgggttc cacgcccgta agagcgccca
     2281 ttcaaaacgc tacgcctatg tgctccatct gacgcgccat cccgatccgt ttctccgccg
     2341 ctacgcctgg gccatcccct gggaactcga cctggccctg ctcgattccc tcgccacgcg
     2401 cattaccggg acgcacgatt tcgcgggatt tcagggcgga gggagcagct gctgccagac
     2461 cgtcaggacg cttttctccg tagcacgccg ccctggaccg atcgccggcc cgcagggcct
     2521 gcccggcgtc tggcatcttg aactccatgg ggacgggttt ctgtacaaga tggtccggaa
     2581 cctcgccgga acgctggtgc acattgcacg ggggaacctg cccccggagc atctggaaac
     2641 gcttctccac cggcccggac cgtaccacgg gtatacggcc ccgccccacg gcctcttttt
     2701 gatggaagtg ctgtatcctg acttcaaata agaaatgcgc cacggtaaac accgtagcgc
     2761 atcctgaaaa agaagacctc gtattcagct tttccaggcg acagaatcac ttcgccccga
     2821 ttcgttctct acgcacgaaa agctccaagg tcttatgcgg cgacctccgt gtctgaagtc
     2881 gaccttgacc tccgtgtctt ggccatcacg cccagtatat cggcggtagc tcccgaaact
     2941 ttagcggcgc ggcgttcgcg gtcaacacat tacgctgaca tcaaaacaga ggaaaaccgc
     3001 ggccggtggg cagcaaaaaa aaggcgccac aactgggaca ttgcggcgca ctcaaggagg
     3061 gtaagcattc gttgcctggg atgcgggaca acaccccgat gcagcagaca gcacatggac
     3121 tcaggatttc tgcccggatg cttacggctt caactgtcgt caatcacggt atcggcagaa
     3181 caggtgcgaa tctttagttt ccggcccgca tgcgtagctt cacacgcggc gctctgctac
     3241 aatcgattcg ttccgtggag ctcgggcaac caaccaacgg gagaggccat ccggatgaag
     3301 gttcttgtag tcggcgcaaa aggccagctc gggcgagaac tctgtctcct ttttggcgct
     3361 gacgccgacg tgaccggcgt cgatcttccg caactcgata tcacggacca cgacagtgtc
     3421 gaaacgctcg tcgcggatac cgcgcccggg atcattatca acgccgcggc atataccgac
     3481 gtggaaggcg ccgaagacaa ccgcgagctc gcctttgccg tcaacgagac cggtgcgcgc
     3541 attattgccg gcgcggcgcg aagagccggc gtcccggtcg tgtactacag caccgattac
     3601 gttttcttcg gcgcccagga cgacgcgctc gagcccagcg accccatcat gccgcgcggc
     3661 gtctatgccg aatccaaggc ggccggcgaa gaggccaccc ggaacgccaa tcccgaccat
     3721 ttcatcatcc gaaccgcgtg gctgtacggc cccggaggca acaatttcgt cgagaagatc
     3781 ctgcgggcgg ccgccacccg ccctgcggta aaagtggtgc aggacgaggt cggttcgccg
     3841 acccacacct tcgacgtcgc cgaggcgacg cgggcgctct gcgctaccac ggcctacggc
     3901 acctaccacg ccgtcaacag cggccaatgc tcgcgctttg aattcacccg ggagatcttc
     3961 cgcctcgccg gcgtcggcac gccgctctgt ccctgcaccg ccgacgagtt cccgatgaag
     4021 gcgccccggc ccagctactc ggtactttgc acgcgtaaac tcaccagggc atgcggctac
     4081 gtgatgcggc cctggcagca ggccctggct cactattttc agcggaggaa agaagcggta
     4141 tgacacggat cgtcgtcacc ggcggcgcgg gattcatcgg ctccaatttc gtgcgcaaca
     4201 tgctcgccgc ctaccccgaa ctgcacatcg tcaacttcga caagctcacc tatgccggca
     4261 atctcgataa cctcaaagat ctcgatcccg cccggcatac ctttgttcag ggcgacatcg
     4321 ccgacaaggc cgccctcctc gacgccatga ccggctgcga tgccatcgtc aatttcgccg
     4381 ccggaagcca tgtggatcgc agcatcatgg gcgccgacga tttcatcgac accaacgtca
     4441 cgggcgtcta caatatctgc gaggccgccc gggaactcgg caccccgcgc gtgctgctcg
     4501 tatccaccga tgaaatctac ggcagcatcg acgaaggctc gttcaccgag gaggatcctc
     4561 cccatccggg caatccctac tcggcaagca aagccggcgg cgagctgatc gccctggccc
     4621 acttcaacac cttcggcaca cccatcatga tcacgcgcgg ctccaacacc tacgggccct
     4681 accagtaccc cgagaagatc ctgcctctct tcgtcaccaa cgccatcgac gagcagcccc
     4741 tcccgctcta cagcggaggc gaagacaacg tccgcgactg gctgtatgtc gacgaccact
     4801 gccggggcat cgacctggcg ctccgcaaag gcctccccgg ccaggtatac aacgtcgccg
     4861 gcggcaacga acggcgaaac atcgtgctga cccgcaagat cctcgacctc cttggcaaaa
     4921 gcgaagacct gatcaaactc gtcaaggatc gccccggcca cgatcggcgc tattccatca
     4981 atgccgaaaa actcagcgcc ctgggctggg cgccccgcat ggactgggac gaagggatgg
     5041 cgctcacggt ccagtggtac cgcgacaacg agtggtggtg gcgcaaaatc aagacgggcg
     5101 agtttctgga gtactaccgg aaacagtacc acgaacggta agccaccccg cgatattctc
     5161 cgcgcctaca tccggtcggg tgctgcgatg ccgagcaatc cgagggcgtt ctggatggtt
     5221 tggcgcgtcg cccggcacag ttgcgcgcgg gctacctgct gctcgcgcgt gggcgccgcg
     5281 atcaccggac attcgtggta gaacgttgtg aatgcccgcg ccgtatcgag ggcgtactgg
     5341 gcgatggggg cgcagttgcg ctgcaccacc gcgccggcca ccaccgacgg gaacgacgcc
     5401 agctgcatca acagcgccca ctccacggcg gtgtcgatcg gaaacgtctc gtccacggtc
     5461 tcagggcaat cgccaaattt ccgcaggatc gacgcgatac gcgcacacga atactgcaca
     5521 tacgggccgg tatcgcccgt aaacgacaag gcggattccc attcgaacac gacgttcttg
     5581 ttggggttca cgcgcagaat ggcgaagcgc accgccgcga gagccacctg cttgataatg
     5641 gcagaccgct cttcagggct cagatcccgg cactgctcct cgactttttc ggcagcgcgc
     5701 gactctgctt cgtccaggaa ggcggagagg agcacgaccg tgcctctgcg cgtggacatc
     5761 ttcccttcct tgagcgtgat gtaggcgtaa taaatgggtt caggcgcggt aaaccccgcg
     5821 gcgccgagga tcaaggccac ctgctccgca tagagcttat gatcttcccc caggacaatc
     5881 aggttgaggt ccgcgccgag gcccatcttg tgaatggtgt acgacaggtc gcggcaaccg
     5941 tacattgagc tgccgttggc gcgcataagc acaaaatacc ggccttcgtc gcgttcgtgg
     6001 cccagtttcg ccagatcgac cactacgcgc tcatcttcat ccgtgaacac ggcatccttt
     6061 tcgcgcaatg cggcgagcac ctgctccaac cgttcgtcgc ggaggtagtc ggattcacga
     6121 tcaaacacgt cgtaacgcgc accaatgcgt tcgagcacag ccagctggcc ccggagacaa
     6181 agctcggtaa ttcggtggaa cttctccacc gtatccggat ccttctcctc catcttgacc
     6241 agaagctcgt acccctgcgc ggcaaattcg ggctcggctt ccgcacgctg attggctttc
     6301 acatagatat cgaggacctg gtcgaacgaa agctgctccg gttcagggca ttcgagcacg
     6361 agcagcccga tctgccggcc catatcgttg acgtaataat ggacctcgac ctcgtatcct
     6421 tcgaatcgca gcaggcgcac caccgaatcg ccgatcaacg cgttgcgcgc ccggcccaca
     6481 tgagggctgg cgttcgggtt aatgctcgta tgctcgataa gcactcgctg tccagcaccc
     6541 gtcctgttcg taccaaacga gtcccctgca tccagcacag acgcgacgat ccgtctcgca
     6601 tacagttccc ggttcaattt gaaattgaca tagggaccgg ccgggctggc ttccacgacc
     6661 tccgggccaa acaggacctc gcctgcgatg cgcgcagcca gttgcggcgg cggacattga
     6721 agccggcgcg ccgcgtcaaa gatccgcagc gctacatcgc ccagatcggg ccggggcgtc
     6781 ggggcaaatg ccaaatccgc ccgctcgatc tctggaaagg cctttgttat ggactcggct
     6841 atggaattga acaggtccac gttacgtctc gctcggttat attgcttgct gccaatggga
     6901 taatgcgcac tgaaacgcga gttgtagcat acgacccgcc atcaagtaaa gaaatcggcc
     6961 cgtccgccgc gaagaaatga cgggatccac cctcggctgc gcatgccgcg tgtggatgcc
     7021 gcctctccag ctccgcggga gactcccatg ccagctaaag aaagcaaaag cgcgtgacag
     7081 gctgagtcca gcaacaaggc gccagggccg cgttaagacg gcgaggggag ggtttcaacg
     7141 atatgaatcg tgcgcgtgac ttcggcggtg ttgccggccg cgtccgtcgc ctgaatgacg
     7201 atcacatttt ctcccagcgt caggtcttcg tcctgttgcc aggtaaccgt ggccgcaggc
     7261 gaggcaaacg ggtcaaattc ggcgagaagc catgcgccat tggcggtaat gcggacagtc
     7321 tccactccgc ttgcgttgtc tgtcacgcag gcctggattc gaggccgccg gtttgtagtg
     7381 gggccggtac cagccgcggg aaccacctct gtgatggttg gtgcgacggg gtctgccatc
     7441 ggggcaaaca cgcccaggct tcccgtgtgt atcgtgagcc gcccaccccc gcgcagggtg
     7501 tcctggcgcg cccagccgtt tcccgatttg cgataaatcg ctatattggc cgcatctgcc
     7561 gcgccgctgg attctggaaa cgagatttcc aacggcacgt cgagcggcgc atcggttggc
     7621 cacaagcggt aacccttgcc catggccacc agcccggacg tgcccgtaac ggtttcttct
     7681 gacatgcgca ggcccagcgt cccataaacg cttcgggcat tggcacgaac caccagatcg
     7741 ccgaaacagg caatgcggcc gcggccgcgc tgaaacacct caatggtctc catgtacggc
     7801 gccagtctgg gatgttccac ggcgagagcc acctggtcgc tgtgttgcgc ggcgcggatg
     7861 gcggcgcgaa acagcccggc gccgacacgc cggaacgccg gctccggctc cgcgcccgtg
     7921 ctctggagca gcggcgtctc cggttcatcg tcgggaaacc gtgcggtaag caggagccac
     7981 tgatcaaagc aatcgatgcc gagcgtgccc tgggcgttgc ttccccggga tggcggcgcg
     8041 ggcggttcag cggcctccgg ctcgatccgt agcggcaggg tggccgtatg gccaaaatag
     8101 tcgcacgcct caatgagcac atccagcggt ttgccgccgg cgtcaattct tccgtcagcc
     8161 tccgatacgg agaaattggg cgactggttg ccttcccagc gccactgcag cagaaacggc
     8221 ccctcggacc gataataggg atgccaggac accgagccgt cgcccatggt ctcgtacgac
     8281 aggacatcgt tttgcacccg gaacgtctcc tgcgcgtcca cgactgtgcg cagggtatgc
     8341 acacccagta cgttcgcgcc tccattggcg gggtcaatca cgtcaaccgc gataccgatt
     8401 ttcccccggg cagtcacggc cgcgcacgta tgcccgccgc cgttgccgtc gggccggact
     8461 tccagcacaa ccggcacgag gtcgccattc agcgtcgcac cgggttccac gggcaccagc
     8521 agcaccttgc ggatgtgcgg gcgcgtggtg tccggccagg taattccgag gaggcgcggg
     8581 ttcagcggcc ggctctgggc atcgcgaaac tcccagtgca ggtgaggggc gcgcgtcccc
     8641 gtgcggcccg caaacgcgag caagtcgccc cgccggacag ggaatgcccc gggttccggc
     8701 tggagatcga ccgcatattt ctcgcgggca tgctgcgcct gccggacgta gtcgcgcagg
     8761 ccgttggtga attccgcgag atgggcgtac acgatggtat ggccgtcgct ggtctgcaga
     8821 tacactgctt tgccgtagcc ccagggcgag cagcggatgc gcgagatata gccgtcggcc
     8881 gctgcgtaga cttccggggt ggccgtgccg gtgcggagat ccagcccggc atggaaccgg
     8941 ccggaacggt actcggcgaa actggaggtg accgctggag gcagcgtgag gggccactgc
     9001 cagggatccc ccgcagccag cgccatcatc gccaggagca tcgtcacggc ttaggactca
     9061 ttccgatctc ctccggcatc tcgcccggat ccagcatatc gagcagttcc ggatgggtga
     9121 ccagcttgcg gtccaggttg agcgccgtct ggatcgcgtc ttcgtggctc gtggcttccc
     9181 ggcagaattc ttccacggcg aggttggttt tccggagcaa cagcgtgttt ttaatgtacg
     9241 gcgcggcttt ttcgaaggtg atttcctcac ccggaagcag ctcgagcaat tcgatgatgt
     9301 ggtagccgaa ctcactttca atgatgccgg acatctcgcc cggcttcagc gtgcgggccg
     9361 cctcggccat gaagtccggc atggtgtcga tgcgcgccgt cccgagatcg ccgcccttgc
     9421 tccggaacgg ctcgtcggac atttcttttg ccaccgcatc gaatttgcgg ccggactgaa
     9481 tccggctcaa cgcctcttcg acttttttgc gggcttcctg gcgctgcgtg gcggccccgt
     9541 tgctgttctg gcgcggcgcc cgcacgaaaa tctggcggag gtgggcccga tccggttggc
     9601 gtgcgttatc cttacgcatt tgctcgaatt gcttcttgac ttccgcgtcg gtgacttcga
     9661 cgcccttttc ttttgcgatg atcccccgta tcttctcaat gagcaatgcg ttgcgcaatt
     9721 ctgcgagggc ttcttcgcgc gtggccccgg cccgtttcaa gacctcgtct tcgctgaggg
     9781 gctcggcctg gccgccggac agctgttggc ccagcatttc cacttccttg gcccattgct
     9841 cctgcacata ctcgtcggtc agcgtcaggt tgcgtttttc ggcttcctgc agcagcactt
     9901 ccttctcgat gagaatgcgc atgcaccgca aggcaagacc gacccgaaat gcatctgaga
     9961 tcgtgtcatc cgggcgcagc tgctggtacc ggttcagttc actcttgtag agttcgacga
    10021 acgcctcgga cggtatgggc cggttatgca ccagcgcgac ggggccggcc ggcacgacat
    10081 ccagcaccag atccagcccc tcaaggttgg gcgcctggct ccatgcggcc agggatacgc
    10141 ccgttgccca tatcaccgcc atcgcgattc gtatcacttt cattcctatt ctcctgtcat
    10201 tccttccggg gcagattcat cgtctgcttc gtcctgcatg gcgcgttcgg cctccgcacg
    10261 cgtgcgcctt cgggccgcgc gttccgcccg ggcctccgcc atcatcgccg agatcagata
    10321 atggaccagg gccgcggtcg ccgtccacga agccgcgaag gtcagcacga cgcggtacgc
    10381 gatctccagg ggctccgccg agctgaaaat gaagtggatc gccatcacca gcgtagcgca
    10441 gaacgatgca acgagcaggc ccagcttatc gctgtcgggc agaataaccc ggaagttctc
    10501 tcttcttctg acgatcgttc tgggcatacc tcatgctacc acagccgacc gtccccgggg
    10561 caactgccgg tgcgtgccag gaagtgtgtt gggcgccctg ccttcgcaac caggctcggc
    10621 tccaatcccc gttttacctt ttccactcag gagatccgct tatggtactc ctgcagcgag
    10681 cgaaccgtcg cggtgtcctg cttgcgcgcg gcaatgccgc gggcggcggc gatggccgcg
    10741 gcggtggtcg tcacatacgc cactttgtac ttgatcgctg atttgcggat atacgagtca
    10801 tcggtcacgc tcagcttgcc cgcgggcgta ttgaccacga gctggatttc gccgttcgtg
    10861 atggcatcaa cgatgttggg ccggccttcg tggacttttt tgatcaactc cgatttcact
    10921 ccgtgctcgc tcaggaacgc ctgggtgccc agcgtggcgc gaatccggaa acccaggttc
    10981 tggaactgcc gggctgcttc gagcactccg ggcttgtcgg gttccgcgac cgtgatcagc
    11041 acggcgcctt ccagccgcag cttcagttgc gtggcttcct gcgcgcggaa aaatgccagg
    11101 ccgaacgaat ccgccaggcc cagcacttcg cccgtcgaac gcatttccgg cccgagcacc
    11161 gggtccactt ccggaaacat gttgaaggga aataccgctt ctttcacgcc gaaatggggg
    11221 aagtgccgcg gccgaagatt catatctgca agcgatttgc ccagcatgac ctgcgtcgcg
    11281 atgttcgcca tcgagatgcc gcagaccttc gacaccagcg gcaccgtgcg cgaggcgcgc
    11341 gggttcgctt ccagaatata taccgtgtcg ttcgcaatcg cgtactggat gttcatcagg
    11401 ccgactacat tcatctcaac ggcgattttt cgggtgtact ccgtgatggt ctcgatgtgc
    11461 ttggccggga tgctgattgg cgggatgacg caggctgaat cgcccgaatg cacgcccgcc
    11521 agctcgatgt gctccatgac ggccggcaca aaagcgtcgg ttccgtcgga aattgcgtcg
    11581 gcctccacct ccaccgcgtt ttccaggaac ttgtcgataa ggatcggccg ttccggcgac
    11641 acatccacgg cggcggcgac gtagtgccgg agcatttcct cgtcgtgcac gacttccatg
    11701 ccccgccccc cgagcacgta cgacggccgc accatcaagg gatacttgat ctccgccgca
    11761 atcgccaggg cttcttcgag cgtgctcgcc atccccgact gcgcctgcgg gatgttcagc
    11821 cggtccatgg tctggcggaa ccggtcgcgg tcttcggcca ggtcgatggt ctcgggcgtc
    11881 gtgccgagaa tgggcacgcc cgcgctcgcg agctctcgcg cgatgttcag cggggtctgt
    11941 ccgccgaact ggacaatcac gccctcgggg cgctcttttt cgtaaatgct caacacatcc
    12001 tcgaccgtca acggttcgaa gtacagtttg tccgaagtgt cgtagtcggt cgagacggtt
    12061 tcgggattgc agttgaccat gatcgactca tagccctgtt cccgcaatgc aaaagccgcg
    12121 tgcacgcagc agtagtcgaa ctcgatgccc tggccgatgc ggttgggtcc gcccccgagc
    12181 accattactt tcttgttggg gctcaccttc actttgtcgg gggcgttgta ggtcgaataa
    12241 taataggcag cgtcctcaac cccgctgacg ggaaccgcgt cccaggcctc ggtgacgccg
    12301 gtgcggagcc gctgctggcg cacccggtcc tcgggcaggc cgagcaggcg ggcgagatac
    12361 ctgtccgcaa atccgtcttt tttggcccgg gccaggagtg cgtcggggag ctctttgcct
    12421 ttgcaggcga ggatttcttc ctccagatcg accagttctt tcatctgttc gataaaccac
    12481 ggcttgatgt gggtcaggcg gtgcaactcc tcgatgcgtg cacctttgcg cagcgcctcg
    12541 tacatcacga actgccgttc gctggtcggg tgcttcagga gcaccatcag ctcatccagc
    12601 gatttctggt tgaagtccct ggcgaacccc aggccgtagc ggccgctctc cagcgaacga
    12661 atggccttct ggaacgcctc cttgtaattt ttcccgatgc tcatcacctc gcccacggcg
    12721 cgcatctggg taccgagctt gtcttcaatg ccgcggaatt tttcaaatcc ccaccgggcg
    12781 aatttcacca ccacgtagtc actcgccggc gtgtaccggt ccagcgtgcc gtcgcgccag
    12841 taggggatct cgtccagtgt gacgcccgcc gcaagcatcg acgataccat cgcaatgggg
    12901 aaccccgtcg ccttggacgc cagtgctgaa gagcgcgacg tgcgcggatt gatctcgatc
    12961 acgaccaccc gtccggtctc cgggtcgtgg gcgaactgga tgttcgtgcc gccaatcact
    13021 tcgattgcct cgacgatggc gtagctgtat ttctgcaggc ggtcctgcag ttcgggggag
    13081 atggtcaaca tcggcgccgt gcaataggag tcgccggtat gcactcccat ggcgtcgaca
    13141 ttttcaatga agcacaccgt gatcatgtgg ttgttcgcgt cgcgcaccac ctccagctcc
    13201 agctcttccc agccgaggac cgattcctcc accagcacct ggcccaccag gcttgcggaa
    13261 agtccccgtg ccgtgatcgt gcgcaactct tcgacgttat acaccaggcc gccgcccgtt
    13321 ccgcccatcg tatacgccgg gcgcagcacc accggatatc ccagcttctc ggcgatccgt
    13381 tccgcgtctt cgatgctgta cgcgagttcg cttcggggcg tctcgatccc cagctggctc
    13441 atggttttct tgaacgcgct gcggtcttct ccgcgctcga tcgcatcgac ctgcactccg
    13501 atgactttca caccgaattc ttccagcacg ccgccgcgcg ccagctccga ggcgaggttc
    13561 agacccgatt gcccgcccag attggggagc agggcatcgg ggcgttcctt ggcgatgatc
    13621 tcacgcaagg tttcgaaatt gagcggttcg atgtaggtct gatcggccat gccgggatcg
    13681 gtcatgatcg tggccgggtt ggaattcacc agcaccacct cgtaccccag cttgcggagc
    13741 gctttgcagg cctgcgtccc tgaatagtca aattcgcagg cttgcccgat cacaatgggt
    13801 cccgacccga taatcagcac tttgcggata tcatctcggc gcggcatagt tacccctcta
    13861 gtggtgcaaa cgttgttggc tcccaagccg gaaggaacgc cgcaccggcc cgggagagtg
    13921 gcgctccgga cctcacccac ggtccggcgt tcagataatc agtgagaatc gcaggctgag
    13981 ccacggagtt gtgcgccctc caggccgcca tcccgcgcag gataccacaa atggagaggc
    14041 gatttcttct tttttgcccg gaatggccgc cggaatctct gtgcgcgccc cctgcaggct
    14101 ggctggacgt ttattcggat ccccatcggt taattccaga gaccggcatg agccgggtcg
    14161 gtttgccact ctcctggacg ccgccctggc ccgcgatcct catttccaca tcaaaagcgc
    14221 aaaaaagccg ctcacacggg agttttaagc acattagcgg ggagtttggt tgacagtgtc
    14281 cggcaggggg gatagaattc tttcaggcgc gtaaatggcg cagaatcagg ggctatgaag
    14341 cggttcagtt tcaacacatt tctcgaggcg gacggcaatc gggcggcgtt gcgcatctgc
    14401 aaacggattg cagagctggc gcctgtctct ccgatgcccg tcctcctgct cggcgaagag
    14461 ggctgcggca agacccatct cttgtattcg atctggaaac gcgttaaggc gacatctccg
    14521 cgcacctcgc ttgccgtggt gactgcccgg gaattccgcc aggaagtgct tgctctcatt
    14581 gacgatccgt cgcccgtcga gaaggccgag tcggccattc tgctcatcga tcagctcgag
    14641 ttgtttgatg tactgctcga tgaactggaa tccgtcgtac gcatttttat caacaataac
    14701 cggtacgtcc tgctggccag caacgtgcat ccgcgccgcc tcaaagtcct gcctccggga
    14761 ttacgcgacc ttatcgccgc aggccagact gtcgacatcc gcttgcgcga gggcgagaca
    14821 cagcttgaag tgctccggcg tgaattgcgg cgcgagagcg aggccgtcat tgaaaagcag
    14881 caggtggaaa tcgatcgatt gcggggattg ctggaacgcg tgggcgcctc ggccggcggc
    14941 gccgacgcct ccgccgcggt ccgggaagca ttggagcagg cacgggcgcg aaacgcggaa
    15001 ttgacccggc aactcgacac cgcgacccgg ctcgggctcg gtcttcaaga agagctggaa
    15061 tcggtgaagt cgcggctggg cgctgcggag cgcggcgaga cgcccgtcaa agcggccgat
    15121 tcagaggaat ccgcagcggg ccgtgccgag cgtgatgaag cgcgcgagga gctcgaagcg
    15181 cttcgccggg aattggcgcg ccggggagat acggaagaag aaattgagca gctccgcgcg
    15241 gcactggcgg ccgcccaggc ggagaaggaa cgcgccctgg ccgaaaccgc tgccgaacag
    15301 gagaccgaac gtcagcggct ggaggcagag gtggccgccc tggcgcaaga gttggtggtg
    15361 gcgcggaacg actccgccca ggcccggcat gaggcgaata tgctcgtcga acgcgccgaa
    15421 cggcttgttt cgcagatcga aggcacccaa cttcgttttc gcgagaccga acagaagcag
    15481 cgccgtcaga ttgaagagct ggaaaccctg cttgccatgc agggccctgc cgagatgcag
    15541 gacgaacgcc tcgccgccct gcaagaacag gtggaagcat cggaagggcg cgtctacgca
    15601 ctgcaacagg atttcgagcg agaacgccgc gagttgatcg agcggctgga acaggacgag
    15661 cagcggattc aggatgagct ttcccgcgcg ctggaggctg cccgcgcggc gcatgaggcc
    15721 catgaggcgc taagcgtgga gcacgcccag ttgcgcgatg ccctgaaaag cgccaaagac
    15781 gagtgcgaat catttcgcac ccagctcgat gcccttgagc aggagcgcga gaccttgcgt
    15841 gttcagctcg aaaatctcga gcaggaacgc gaatcatttc acactcagct cgaaaatttc
    15901 gaacaagagc gcgaatcatt ccgcacccag ctcagtgatc tcgagcagga gcgcgagaag
    15961 ttgcgcgttc aggtcgaaaa tttcgcgcaa ggggacgaat cgttacgtgc ccagcacgat
    16021 aatctagcgc aggaacgcga ggcgttgcgt gcccaactcg aacatctcga acgagagcac
    16081 gaatcgctgc gcgcccagct cgaaaatctc gagcaagaac atgaatcgct ggggtctgac
    16141 ctgaatgcca gccgcgctga acgcgccgcg gcggaatcgg cgctggagga actccgtcag
    16201 acgcgcgaaa acctggaatc cgaactgaac gggagccgcg ccgagcagga ccgcctgcgc
    16261 gaagagctcg aacaagctca tcgtgaaaac cagcggcggc aatcagccct cgacgaatcc
    16321 cgggtgcagt tggaaacggc gcaatcccag gcgcggcagc tggaagcacg cctgagcgaa
    16381 caggcggccg acatggaagc cctgcgccac gatgccgcgg cccaggtggc ccgcgccaat
    16441 gcccaggcag gcgagctcga aggccaggtc gcgcagttgc gggcccagct tgttcaagcc
    16501 ggcgaaagcc gtcgcgaagc cgcccgccgt atcgaagaaa tgcgcgcgaa tctcgcagct
    16561 tccgcgcatg tcctcgggga atggtcctcc cagcttgcct cccacgaagc gcctcccgaa
    16621 gcatccggcg aggaactgga tgaggcttcc tgggcgccgt accaggactc tcccaaagca
    16681 cgcccggctg ccgccggttc agcgcaggaa agccagaccc tgccggagat tgatgaagac
    16741 gaccagttcg atggcgatcg aatggtattg ccgcccgccc ccgatgaaga agatgtcgag
    16801 cccggcgctg gctatgggag cgatcccggg gccggcgctc cagtctggac cgattcggag
    16861 ccgcccgcga attttgacgt gccctcaccc cctctcgagc cgctccagga cctctctccg
    16921 ctcgaagacg agtgagacta gccggcacgg acttactgtc tgaaaacgcc tctcgtgaaa
    16981 tgcgccgcgg gtggaggcga ggccgcatgg cgcgagggga gaccgttctg gcatactcct
    17041 ccctatggac acactcgaat cacgcatcat ccgcctgttg actgagatcc acccgcttga
    17101 ccgcgttccg cgggcagggt atgtgctgcg cggcgtgccc gaaccggaat ccgtggcggc
    17161 ccacagccac tttgtcgccc tgctggccct gctgatctgc gatgcatgcc cgggccagtt
    17221 tgaccgcggc aaggctctga ccatggccat catgcatgac ctggccgaag cccgcctcat
    17281 ggacatcccg atgaccgtca gcgatgctta tttgaagggc gccaagcgtg acgcagaaca
    17341 ggccatcttt gaggaccttg tcgcaggatt ccccgagcgg tatgcccagt accaccgtga
    17401 gctgctcgat gccgtctccc ctgaggcccg tctcatccgc gggctggaca aggcgcaaat
    17461 gatgctcaaa attgtcatgt atcaaaaaga aggccggggc cgcctcgacg agttctggaa
    17521 taacccgcgc aacttcgccg atttcggcgt cgagcccgta tcccggcttt tcgacgcgat
    17581 ttgcgcgcat gccggcaggt gtcgcccaaa atagatacac gggtgcacac ttgttattca
    17641 tttttgacaa ccgcccggag gctcacggat gtcccagggt ccttgtaatg gttttaattg
    17701 aagtaatcgt gtgtagtggc ggggtaatcg gctctagaaa gccgtggaga gattggcccg
    17761 atttctgcgt atacatttgt cgctgaggtg tgtctgctcg accgatgagt agtcggatgc
    17821 gcatttcggg ctggagaacc gcgcggcgtc cgcaaggtcg ttatcatctc acacgcgggc
    17881 gctcgggtga gtccagggga gactgatgca gcgaagccgc gcaaacgctg acggcgaaga
    17941 tgtcacaacg agagcgtcag ttacgcatac tcgcatccac gtgaacggtg tccgggcgct
    18001 gtcacgcaac ggacgggagc aattgcgtct gttggagctg caccttgttg ccctggagac
    18061 cgatccttgt gatatggaaa aaatcggcgc ggtggtgcgc gcgctgggga atctccggcg
    18121 ggcggcgcac cgcctcaagc tccgcgatct gtactattgt gcagaggagc aggaacgccg
    18181 tgctcgcgag gcgcttgaag gaggcctgct tccggagacc accatcgatt ccagcctcga
    18241 gatgattgat gtgctgaaac ggtacctcgc cggcgtggag gaatcgctgc gtacgggaga
    18301 gccgttgcca tcgccgcgaa tgctgccgtc gttgcggcgc agcgcggaat cccggcgcgt
    18361 gcccaggcgg atcgcacgga ccagcacgcc gcgggatgcg cgcaagcgac tgggcgagct
    18421 gctcatggaa tccggcgaca tcagcgaagg cgagcttcag cgcgccttgt ttgagcttga
    18481 agaggcaggg cctcccaggc tcatcggcga attgctgttg gaggagctgc agatttcacg
    18541 ggagcagctc gatcgcgcgc tcgccgggca gcaggatgca tctcagtccc tccggctggg
    18601 cgagattctg gtgagcctgg gcgccatttc gccgcagggc ctggtccggg gcctggaaaa
    18661 acagcgtcat ccaaaggcgc cccgcctcgg cgaacggctc gtccggacgg ggcgggtgcc
    18721 tgcgaagcgg gtggtgctgg ctttgcgctg ccagggcatg attcgcgatt cggtccgcca
    18781 cggcctggcc acgctggcgg cccgattcgc cacacgcgtt accgaaatgg gccctccaca
    18841 gcaacgggag cgcctttccg cggacgagcg cgcggcgctg tcccggtttc gagacgaggc
    18901 gcgaaatctc ttgaatgcag ccgatcgcgg cctgctcgcg ctggaatcgg agtccacgga
    18961 tccccacgcg ctgcggtccg tccgccggtc gatttatgct ctgtcccggt ttgccagata
    19021 cctgggtctg gccgatatcg gccatttcgc acgggcattc tccggctacc tggcgcgggc
    19081 ggcaggcaac gcgttttcgc tggagggacc gcgtcttgac gtggccttcg attgtatcga
    19141 cgtactccgg cgtcatgtgg actatgttga cgacgccttg atgacccagg gccggatacg
    19201 ccgagagcgg caattgcccg agtacatcgc tcttttgcgc agcctgagtg tcggatggca
    19261 gaaagatctg cgggtcagcc gtttacagcc gtccgagcaa gggcagcggc tgggcgatat
    19321 cctgatggcc tccggtgggg tcacacgcga ggcgcttgac gccgccctgg ctgctcaggc
    19381 ccggacgcag gaatccgcca ggctgggcga actgctggtc gatgaagcgc tgatctcgcg
    19441 cgaccaggta aacgcgatcc tcgctgccca gcagcaggat gtgtccatcg gacggctggg
    19501 cgatgtcctg gttcagtggg ggctggtgga ccgggccgac atcgatgcgg cgattgcgcg
    19561 caggcaggca cagggccggc cacgcctcgg tgaactcctg gtgcggatgg gggccgttcg
    19621 cgcgaaggcg gcagcccatg cgttgcggcg ccagcgcgcg agactcgccg gcgctgcggc
    19681 ggccgtgctc gtggcgtcct ctatccttgc accggcttcg cctgcagccg cgagccccgc
    19741 tcttgccacc ggtgattccg ccatcgcaag agctttggac caggggcttg cggccggcgc
    19801 ggaacgggac aggctgggcg gcgccgtcga ggccgcgctc gtcacccggc ccctggctct
    19861 cgatatcgag cgggatggta tgctcgatgg ctgggaggtc tggaatgcct tacatcctgg
    19921 acgcagcgcc ggcatggccg gagccgcccg gcgcaattcc ctgacgagcg gaattgagat
    19981 cgcgccggcc tcgcagcaag agccggaggc gtccagcgag gcggaagcct gggattatat
    20041 cgaatggcag cgcatcgcag atcccgcgtc tgccgaaagg caccccatgc cgcaggcccg
    20101 tggcgagctc tctttttccg atctccagta tgtcatcaac gccgcgctgg gcctggacac
    20161 gcccgtgcct cccaagctca accgggccgg agcagcggat gcgccggata tcctgggcat
    20221 catccacgcg gcactgggca tcgctccaca ggcgtgaaga cgtgcgcccc gggcgccctt
    20281 attcgccgat gatcttcacc agtacgcgct tccggcgctt cccatcaagc tcgtaataga
    20341 aaatctgctc ccagggacca aaatcgagct tgccgtccgt gactgccacc acgacttcgc
    20401 ggcccatgac ggtgcgtttg aggtgggcgt cggcgttgtc ttcataccca ttgtgggcat
    20461 attgatcgta aggcttttcg ggcgcgagtt tttccagcca gcgttcgaaa tcggcatgga
    20521 ggccggactc gttgtcgttg ataaatacac tggcggtgat gtgcatggcg ttgacgagca
    20581 ccagaccttc tttcaccccg ctttcccgga ggcattcctc gatctgcggc gtgatatgaa
    20641 tgatctgccg gcgttgcgtc gcgctgaacc ataattcttt ccggtaactc ttcatcacgg
    20701 cgatctcctt ccctcaggcc gctgattcta cccaatttca ctgctgacgc gcacaccgcc
    20761 gggcgtccgt cccatgtgaa catggactcg gcagcccgtt cttgtttgaa tggctgagtc
    20821 tgatatgata ggaagcactg tccggcccgt ctgagccgac gcggtttgtc ttcaccattc
    20881 gcaaccagga gaaccaccat gaaacatcaa aacttgacgc gtagggcctt catcgccacc
    20941 agtacgctcg cagcctccca attgggttgt gccacggcgc aaactcggat cgcgatgaac
    21001 gacgcgcagg tcgttcccgg caaaatatcc ccaaacgaga gactcaatgt ggcggcgatt
    21061 ggagcgggcg gcaaaggttt ttccgacatc atgtcgtgca agggcgaaaa catcgtggcc
    21121 ctgtgcgacg tggactggaa acgctgtgag aaagccttta atgagctgcc cgacgcgcgc
    21181 cggttcagag attaccgcaa catgctcgaa cagatgccgg agatcgacgc ggtcacgatc
    21241 tcggcgccgg atcacattca cgcaccagcc gccatcctgg cgatgaagat gggcaagcac
    21301 gtatacgtgc agaaaccact cacccatacc gtgcaggaag cgcgctggat gacgctggtc
    21361 gcccgcgaag ccggcgtggc gacgcagatg ggaaatcagg gacattccgg caacggcgtg
    21421 cgggaattgt gcgagatgat ctggtcaggg gccattggcc aggtgcgcga ggcgcatgtg
    21481 tggacccacc gccccgtatg ggcgaatcag ggccggccgg atccgctgcc ctccgccgtg
    21541 gcgcccgagc atctcgactg ggaccggtgg atcggccccg cacccatgcg cccctacgca
    21601 gaaggttatg cgccgcatga ctggcgcgcg tggctggatt tcggcggcgg ttcactgggt
    21661 gacatggcct gccatatcat ggacccggtc tacatgtctt tgaagcttgt cgaggcggcc
    21721 agctacacgg ttgaggtcgt gatgcaggag ggccggaatg aagagacttt cccgctcacg
    21781 acgacgatca aatattcatt cccggcgcga ggcgacatgg ccccggtgga tgtgtactgg
    21841 tacgacggcc actgggaaga ccccaagacg ggtgaaaaaa tgtacaaccg gccgccacgg
    21901 cccaaaggca ttcccgaaga ccaggaactt ggcgacgaca acaagaatgg ctcgtacctg
    21961 gtgggcgaca agggcatcat gacccagggc gagtacggcg gccggccgcg cctgatgccg
    22021 gatgagaaga tggcggatta cacgaagccg gaaccgttcc tggagcggat acccgatcag
    22081 aaccattacc aggactggct gcaggcctgc aaaggcggga aacctgcctg ctccaatttc
    22141 gattacgccg gcccgtttac ggaaatggtg cagtttggca atctcgtggt gaaatcgggc
    22201 aagaagctgc actgggacaa caaaaaaggc gtcgtcacca acgtgaaaaa cgccgaagca
    22261 gtggtctcga aggaataccg gaaaggctgg gaactgccca tctgacgcaa cgcgaccgta
    22321 cgtgcggatg cgccgggagc ggatgccggt gggtgttgat gagatgaaac gccgcatggc
    22381 tgcgcgagcc gaatgcgaag gcgccggcat cgcgcgcgtg ccgaaatacg gtgtgctccg
    22441 ccccgggcaa cacgacagcg agactctcgc tgcgctgttg agccgggtgc gggcatgccg
    22501 ggagtgcgcg gcggaacttc cgttggggcc gcggccggtg ctgtgcgcca gcgaaacggc
    22561 ccggatcctg attgtgggcc aggcgccggg cacgcgagtg cacgccacgg ggatcccgtg
    22621 gaacgatccc agcggggatc ggttgcgtga ctggatgagc gtggatcgcg acacgtttta
    22681 tgacgtgtcg cgtattgcca tcataccggc gggactgtgt tatcccggcc gctacgaccg
    22741 gggcggggat ctgccgcccc ggaaggagtg tgcggaacgc tggctcgaac cgctattgag
    22801 gcacctgccg aacgtggcgc tgacgctctt gtgcgggcat cacgcgcagg cgcttcatct
    22861 gggagtgcgc gcaaaggaat cgctgacgga aacggtggaa tcctggcgcg agtattttcc
    22921 gcggtacctg ccgctgcctc atccgtcgtt tcgaaatctg ctctggatga agaagcatcc
    22981 ctggtttgag cgcgagctgt tgccggtgtt gcggaagcgc gtgcgcgccc tgttgcggaa
    23041 acggtgaccc ggttccacgc gggccggaag aggcccggca acagcatccg ggcatgcggg
    23101 gcgcctgcgc ggcgtaatcg tgtgggttgg gagtgcgccc gtcccccggg cagatcagga
    23161 ggcattacgg catgggctgg gaagcctggt tttcgtttgg aattattggc gccattctct
    23221 tcggcctcgc cacgaatcgc gcctcggatg ccctgctggt cggtggtgtc gtgttgatgg
    23281 gacttgccgg gatcctgtcg ccctcggaga tgttcgcggg attttcgaac accggcatgc
    23341 tcacggtagg cgctctttac attgtcaccg ccgggctgcg cgagacaggt gcgctgcaga
    23401 gcgttggaga ctgggtgttg gggggcgcgc gcaacgagca tcacgtgctg cggcggttga
    23461 gctgggtgac ggccatgtcg gcgtttctga acaacacccc catcgtggcg atgttcattc
    23521 ccattgtgga agactggtgc cgtaaacacc aggtttcgcc gtcgcgcctg cttatcccgg
    23581 tttcctattt ctccatcctc gggggcacgt gcacgctcat aggaaccagc accaatctcg
    23641 tggtaaacgg cttgatgatc caggccgcaa agaacgatcc cagcctggca gatcgcctgt
    23701 tcgagatgag tatcttcgaa ctgggccgcg tggggttgcc ctacgcgttc gtcgggaccg
    23761 cgtacctgct cctggcgggg ccgcgccttc tgccgcagcg gcagggggcc cgcgaccggc
    23821 ttggggcggc ggcacgggaa tacctggcga acatgcgtat cgagaccggg tgccggctgg
    23881 cgggcaagcg agtcgaggaa gccgggctgc gccgtctcac gggcctgttc ctgatcgata
    23941 tctggcgcaa caatcgcatg atcgcgccgg tgggcccgga tgaagtgctt gaagaaggcg
    24001 acatcctgac tttcacgggt gtggtggacc acatcgtcga tctggaacgc atcccggggc
    24061 tcgtggcgtt cgttgacgaa gactacgaag cgcaggccgc tgcgcgccgc catgaaatgc
    24121 tctgcgaagc cgtggtctcg cgaacctcgc cgttaatcaa caagaccatc cgcgaagcca
    24181 atttccgggc gcgctacaat gccgccgtcg tagcggtgca ccggggcggc caccgccttc
    24241 aggggcgtgt aggcgatatc gagcttcaac agggcgatac gctgctgttg caggcggggc
    24301 cgcatttcgt cgatgcgcaa cggaacaaca gcgacttctt cctggtcagc ggagtgagta
    24361 attcgtgtgc gttgcgggac gacaaggcgt ggctttccct gggtctgctg ggcctgctcg
    24421 ttctgctgat gacgacagga aagatggaaa tcgtgctggc ggcgttcctg gtggcggggc
    24481 tgatgatcgc gacgcgctgc gtgtccgtgg cggatgcccg ccggagcgtg gactggcaga
    24541 ccttgatcac gattggcgcg gcatttgggc tgggcaccgg actggtgaat tcgggagccg
    24601 tggaggcgat ttcggggcgg gtggttgcgg cgatggggcc ctgggggccg cgggcggtgc
    24661 tgctgggggt ctatctgatg accacgttgt tcacggagat catcaccaac aatgcggcgg
    24721 cggcactcat gttcccgttt gccgtggcga tcgcccaggg gctgggcgtg gatccgcgcc
    24781 cctttgccat gacgatcgcg ttcgcggcgt cggccagttt catgacgccg ctcgggtatc
    24841 agaccaatct gatggtctac ggcccgggcg gatacagcct gcgcgatttc gtgcggattg
    24901 gtttgccgtt gaacgtcctg ctgatgttga ctgcgacgct tctcgtgccg ctcgcctggc
    24961 cgttttgagc tctgaagccg gccctggcgc gtcaatcaat tggatggaag agctcgctga
    25021 cggcatcgtg gagcgcattg gcgagcacga tgcgatcatc ccggatgatg gggtctttgc
    25081 ccagcgtcag cgtaagtcgg tggtacggga ggctgagcag ccggagcagg tgtggcagga
    25141 acggccgggg cggcccccac atctcaagct gcgcgggagg aatctctcct tcgggcgtgc
    25201 ggtagtaggg atcggggccg aacagaaccg attgtgacgg gggcggccat ccccacgggg
    25261 tgtgataatg gatggcgccg tagtggaccg ggaaccggtc ctgggcggcg cttccgagca
    25321 gggcggcccg gaagcgctga atgctccgcc caggggaaat gacgctttcc ggagccagca
    25381 taatgctctg accgcgcgcg agccggcgcc ggatctccgc gttcactcgc ggcacctctt
    25441 cgggatgccg gctcacgaac acatagtcca gtgcgcggaa aaagcggccg agcacgggca
    25501 aaacgcggag ggagtcaatg gcaacgagcc gcccctgcgg gaaaagatcc tgaatcagga
    25561 aatcttgcca ggtgacgtga ttggagacaa aaaacaactg gccacggggc acgggcccgt
    25621 acgtggtgac gcgctgtccg atgcctttcc ggagctggcg gatccaggcg agggcgatgg
    25681 ggcgccggtg cggctcaaac cgggccggag acagccatgc cagggtatca acagcgagca
    25741 gggctgcata acagcatgcg ctagcagcga ggaggcgcgc cagccggaac gtcgcgcgca
    25801 ccaccgctcc cggcatccgg gccggatatg cggccgggta gtctgccgag cgcggcggat
    25861 ccgcccgatg gttcattctg cggcatcctc atcgtcggca agcgtcaatt ccatggtgca
    25921 ttgtatggtc tgccggattt cttccgacgg gaagggtttt tgtgccagat ccgccagggt
    25981 ccagcgtttc agaatggtga tgatcgcctc atgaagttca gccatcgctc ggtgaaacgc
    26041 gcagaccagt tccatgcgat cgaaatcctg gcagaaatcg ccgagaattt tgccctggca
    26101 cgcttccacg acatcgagca gggtaatggc ctcgggcggc cggctcaggg tgacgccgcc
    26161 tttcataccg cggtgcgcgc gcagaatatc ggctttcacg agagccgtgg tcaccttggc
    26221 cgtgtagctt ggggaagcat tgatgtgctg cgcgatgcgg gcaggcggaa cgggcgccct
    26281 ggcttctctc aaaagcagga atacgaggac ccgcagggcc gtctccgaag tctgcgtcaa
    26341 catggtcggt cgtcctgttc gcgcatatgc ccataccata gcgaaaaggc ctcagccgca
    26401 acaaacgcag tcaaaaatgc gtgtttcgcc cggcgacaaa ggcgggcggc acaagaccgg
    26461 cacgggaacc gctccgcggg ccgaaccccg gcggagcggt tcccaagccg tgttttcgca
    26521 actcaggcgt tggccatgac ggtgttcagg tcggccatcg cgtccttgcc tataggttga
    26581 aggtcgaact tttcctgcag aagagccagc acgttgggcg agacaaacgc aggcagggtc
    26641 ggccccaggc gaatgccctt gacgcccaga tggagcaggg tgagcagcac ggccaccgct
    26701 ttctgttcaa accacgagat gagcagggtg agggggaggt cgtttacgcc gcattcaaag
    26761 gccttggcga gcgcgagggc gacctgaatc gcgccgtacg agtcgttgca ctggcccatg
    26821 tcgagcagcc ggggcaaacc ggcgacggtg ccgtattcgt gttcacggat gcggtatttg
    26881 ccgcagccca tggtcaggat gatggtttcc tcgggcgcgt tgtaggcgaa atcgcggaag
    26941 taattccggc cgggatcggc cccgtcgcat ccgccaatca cgaagaaacg cttgatcgtg
    27001 cccgacttga ccgcgtcgat gatcttgtcg gccagatcga gaatgacgct gtaatggaac
    27061 cccaccgtcc gcgtgaccag ctcagtgggc gtcagcgggc cgatctcgag ggcccgctta
    27121 atcacctccg aaaagtcatt ggtggtgatc cgtttcgcgc ccggcaccgc ggtgacccgc
    27181 gtggtataca gccggtccag gtacgtgttg atggccggat tggggatgag gacacagttg
    27241 gtcgtggcca gaatgggccc gccaaacgcc tcgaattcca tgcgctgttt ctgccatgcg
    27301 ccgccgtaat ggccggcgag gtgcgggtat tcacgcagtt tgggatacat gtgcgcgggg
    27361 atcatctcgc cgtgagtata gaccttgatc ctcgtgcctt tggtctgctc gagcaggtca
    27421 agcagatcga ccatgtcgtg cccggtgatc aggatgcccg gcccgtcctg gatgccttcc
    27481 tggatgtcta cgggcgacgg tttgccaaag gtgtcgacat gcccgtcgtt caggagctgc
    27541 atggtccgga ggttcatctc gccacactgc agaaccagtt cgagcaggct gtccatgtca
    27601 aaattgacgt tggttacggt ggcaaacaag gcatcttcga caaacgcatc caccacgggg
    27661 tcggttttgc ccaggcgtcg cgcatggtgc ttatacgaac acatcccctt gaggccatag
    27721 aggaggattt tctgcaggga ttcgacatcc gcgttctttc cgcacgcgcc ggtatcggtg
    27781 caggcgatac cgtcgcgggt ttgttcacac tggtcacaat acatgaggct catggttgta
    27841 ttctccttga ttgacgggtc agccacccgc aggcaaaaga tggacatatt ttatccgcaa
    27901 tatccgctat tttcaaaccg ccgcctgccg ccatccacgc ttctggggtt gccctggttt
    27961 gagggacacg caggggtaga taaaatggag catggagatc cggcgcggcc aaacggactt
    28021 atgacgaagc gttcaagcga gatgccgtgg agttgctgct cagcggcggc caggatttga
    28081 agcgcctggc ctgcgcgcct ccgaagctct ctcatcctcc acgcggagca cggttgaggc
    28141 acaagagcaa gccggttcct gcctctcccg catcaacatc gagcgccctt gcaggcccgc
    28201 tgaacgccgc gaaggcccgg ctcccggtga ttccctgacg cagacccggc g
//
LOCUS       DAOPBQ010000002        21720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_2555_length_21720_cov_4.124486, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000002 DAOPBQ010000000
VERSION     DAOPBQ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 21720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21720
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2555_length_21720_cov_4.124486"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKW60_00105"
     CDS             <1..969
                     /locus_tag="PKW60_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97858.1"
                     /translation="GGFGWFPTQWSDTWDTQPVFSLDPTTRVTMVGSLIATTVWWVCT
                     AGSDQMAIQRYLATRDAKSARRAFLVNNIADVCVNVTLISLGFALLGFFRTHYPGLLM
                     SALADEKSADYLFPLFVVNFMKYGMAGLVIAGMLAAAMSSLSSGLNASATVFNTDFLP
                     LLFRKELDERGKVRQGMAVSAVIGVIIILLALVIENVRGNLFEVTQKTSNLLVSPLFS
                     LFFLALFVKRANTFCAMYAAAYGFVVAVVIAFWDLTGATPISFQWIGITSLLASVGAG
                     VGLSLLPWERMTRNSRLGAGIAAALPLVILAGFFIAGIAARPGAAG"
     gene            1100..4768
                     /locus_tag="PKW60_00110"
     CDS             1100..4768
                     /locus_tag="PKW60_00110"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPJ97859.1"
                     /translation="MNRRVHICVAMLLLARAALGQAPSQLWTEATIYRDNWGVPHVYA
                     ENPHALAFAFGYAQAEDRLETIMRAYRLVTGRAAAVFGPDYAESDAFALKLGHSDLAV
                     HALESASPLTLDLCEGFALGINAWIADHPARVPDWADGARPADVLALMHCYLMSFAPF
                     DLPELFHRPAPAYSGNAWALAPSRTLSGAAVLVLNPHGYYDGPFQWYEAHLAGPQVNV
                     AGATLCGLPVILQGHNEVLGWALTPNYPDFADIYTMPEGEIRLPPGDPNRMQLQAKLE
                     RLLIGRQVMESRVYFVGTPSGMRQRSVPFRLTARGPVVGEIGSEPCTFMAGGYADFET
                     LDQMMAMAAARNLGEFRNALGMRQLPCFHVVYADRAGNLFYLYNAKVGDKTLPPALAR
                     GPELEPGSGEGPLGPHAAPYVWDAPVPANNPQFAWGELVPLGALPTIVNPPAGFIQAC
                     GTPPWGATLNSGIAPASIPLWLANERDTFRAQRARHLLSMGSRSFHEAQAMVYDVLVP
                     FATAAVPQLIQWADAREHFYETAHPDTAHGADILRSWNFVAETGSAGMTLFNAWWAAY
                     RQLAGPHLPDVILYQEFVSKPPHVERLALEALSNAAREMRNVHDALDVPWGDVHRMRK
                     GPQEAPVAGGEAGEPLMVMGDYGIARRKLLPNYGFAYAMAIQFGDTPESVSILPGGIS
                     DDPSSRHFADQLKLFSERRFKVNYFLDKDVQRYAERAFGRRPTLRPVGMQGVFGIEAR
                     DPVSIHLECSPNAPAPMPDGMAPFTIFVTPVLDSVHAAVTIDAAIYVPPSVVETAGLQ
                     RLAVCFLDPAHGWFPAPEQQVNPETRIFRARLEGTPTCAVLGPASLRARLESPPAAPA
                     RAEEASPAPVQETPAEPASALVETDEPEAPDAPPAESEPPAPPPLAPAASAAPGVPDG
                     ALRSGLAWGKSLEIAIPGGAGTVRLQASDSLGAFVSASTEPPAPVPEGLSPITPFAGV
                     HCSDPAVSVTLQLELTVPGITAANASEVELLAYRAESGWQALSDQHFNAEAGAMMGSD
                     DQSGVYAAFARGAASAMPPAEIQTQEMPSAAPVEIAPALPAAVPTEKAAQDHAAAEPA
                     SESRKPALAWGKTLELMSENHSAEFRLEADASIGAFTKVLSEPPATLPEGLQAYGACV
                     ALSLSKRDVPVRITVTLHVGSEIPQGLDPASLVLYAHEADTGWARLEGQKFDPAARTV
                     SAPDHQPRTYALLGPKGS"
     gene            complement(4861..6675)
                     /locus_tag="PKW60_00115"
     CDS             complement(4861..6675)
                     /locus_tag="PKW60_00115"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPJ97860.1"
                     /translation="MTQRLRRPRFVKNLPLLLLLVALTGSPPVYAGVMRVPLAPGVSA
                     ASRNGRSLLLEYNRAAGPAPRAFADKYLADSSAWKTYQGMTSSIAVPFDELNPAAQRE
                     VLLAVFPYDVVTPDGWTHYVLHEEDSGPEALWTLCRWLTGKGSTCKEIQKINKLETDK
                     LRQGQSLFFPSILLLDAMKKPTGERPAPPGLLAASPLAPAQEPTDTPLHALAASAAPG
                     ANELEYSRDARGAYALYRLKRGEALYTDVVARFTDVDVQESAAATILAACSVVMERSG
                     IRDERSIPVGQAIKIPMDMLSAQYMPEGSSERSEYQAMVLEARRLRGAVRSKDLAGVV
                     VILDPGHGGRDHGAWTTNNRFSLYEDEINYDIACRIKQILESQTQATVHLTVIDRIQG
                     YTPTDRTRFVHDTNEELLTSPRHRDLDGDKMSLNLRWYLANSIYRAEVARGVDPKKVV
                     FSSIHCDKLHPSLRGAMIYVPGAAGRRDKEAPALTLASYDKYKEVGEQNYASSTAAER
                     RRDEALSRNFATVLMDELGKKRIKRHDRSAPIRAQIRRDGTSFVPAVLRNTMVPTKIL
                     IETANLANDVDCARLEDPEWRQWFAEAYVNALKVYFAG"
     gene            complement(6750..7388)
                     /locus_tag="PKW60_00120"
     CDS             complement(6750..7388)
                     /locus_tag="PKW60_00120"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPJ97861.1"
                     /translation="MTDEAVRNEDTPDLSKGRECLVFLMGQHFLGRILDASEDRLTLT
                     FPGTDYPTPGMQLQLQFHDEEGFNSYLSLVVAGPQEHAGCVIVERPAAMERILHRQNC
                     RVSTDLTVQIKEAELIRQYDAALLNLSGGGALLETDAPLDLNSSVEMTISIPLDTVRT
                     IHGRVTHIERLVPPHDSCRFIVGVCFGKMDAATTRAIKEYVWQRLRELYPGH"
     assembly_gap    7960..8059
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8444..9286
                     /locus_tag="PKW60_00125"
     CDS             8444..9286
                     /locus_tag="PKW60_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ97862.1"
                     /translation="MMLERMPQEVSYLYILKLSELLVLTGVAIAIAVAGYAVFRVWYQ
                     RDKVPPLWLIVNAGILALALYLIAAIPIWVRADAYRKLTVVLYSLVIGVIPGAYFSYS
                     FLSAMALRSVRRITWLDTSLPGVDEFLQARELAAQGDIDGAVAMYENYPEKQGHALFA
                     AAHLLEMNGRFQEAAEMFQKCAEISGEQLRPWSEALFRWARLCEVNLDRIDKSIELYE
                     QLVERAPSTELGRLASTKLVRLRTKTGARASARASRASATAPSRKKAARQRSKKGTAQ
                     KSKK"
     gene            complement(9414..10091)
                     /locus_tag="PKW60_00130"
     CDS             complement(9414..10091)
                     /locus_tag="PKW60_00130"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPJ97863.1"
                     /translation="MGRSKAKYELGHAHLERLVHYYHFLGERVDLDETTRVSSQQLAQ
                     LLTMDDTQVRKDLAAIGVRGVPRLGYRAADVVDAIRQALGFDTIHRAILVGAGRLGGA
                     LAAYRGFVKYGLRIVGVFDQDPGKVGTTIGTHFVYNTTELETVIERNNVRLAILAVPA
                     EACQALADRLVRAGIAGIWNFGPTTLTVPSGVVVRHEHIVVGLAELAYHLQHEPECHG
                     AVPHETE"
     gene            complement(10127..>10771)
                     /locus_tag="PKW60_00135"
     CDS             complement(10127..>10771)
                     /locus_tag="PKW60_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPJ97864.1"
                     /translation="REAGADRYLLRFETSDRALYRTIHPDARGQVSDRFAILAQLRQL
                     GYEIGSGVMVGIPGQSYESLAHDIHTFRTLDLDMIGAGPYIEHPETPLGQGDWSRPCV
                     DGEQVPNTELMTYKVVALTRLVCPEANIPSTTALATLNRAGGRELGLARGANVIMPNI
                     TPPRYRRLYEIYPAKACFDETATSYHADMPAWLEAIGRVPGHGQGGRVRARPRK"
     assembly_gap    10772..10781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10782..11217)
                     /locus_tag="PKW60_00140"
     CDS             complement(<10782..11217)
                     /locus_tag="PKW60_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPJ97865.1"
                     /translation="MMTRLSNPSNSELSEAGIQAWLREEDSARLEELWALADRVRRAA
                     VGDEVHLRGLIEISNHCIRACGYCGLRSGNREVARYRMSEDEILSCAADAVQYGYGTV
                     VMQAGEDYGIDADWLAGIVRRIKTETALAVTLSLGERPFEDLK"
     gene            complement(11239..12528)
                     /gene="hydF"
                     /locus_tag="PKW60_00145"
     CDS             complement(11239..12528)
                     /gene="hydF"
                     /locus_tag="PKW60_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPJ97866.1"
                     /translation="MQRAPKSFRLHIGLFGRRNVGKSSLLNAITRQQVSLVSHHAGTT
                     TDPVEKPMELLPLGPVLFIDTAGIDDEGDLGELRTQRTRQVFERTDLGVLVTEPGAWG
                     TFEDEILDQLARREIPAIVVFNKMDLGRPEPGLLATLRERGVPVVLTAAATGAGISDF
                     RQALLNRAPDEFVNNPAILADLVGPGEMAVLVVPIDKEAPKGRLIMPQVQSIRDLLDG
                     DAFCMVVKDRQLGEALRRLSVPPKLVVTDSQAFREVARDTPREILLTSFSILFARFRG
                     DLVQQTLGAMAIESLQPGDRVLIAEHCSHHPIEEDIGRVKIPRWLTQYVGGPLEFEWV
                     QGHDFPEEIAPYQLVIHCGACTANRRAILNRILKCREAGVPITNYGLTISYSLGIFER
                     ALEPFPEALEAYRAAKTDCGVQAGAAVPETMPAADHL"
     gene            complement(12770..14212)
                     /locus_tag="PKW60_00150"
     CDS             complement(12770..14212)
                     /locus_tag="PKW60_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPJ97867.1"
                     /translation="MRRREFLGLLAAGASTAGLGWFTRRASADGRRPNIVLCMADDQG
                     WGDMAYNGHPVLQTPHFDAMAAEGIRFDRFYAAAPVCSPTRASVMTGRTPNRMGCFRW
                     GHSLRPQEITVAEALKAAGYITGHFGKWHLGSVQRGSPVNPGASGFDEWLSAPNFFDN
                     DPILSREGQAVQFQGESSMVTVDAAIAFMQKHVDSPAPFLAVVWFGSPHNPHKALDSD
                     LELYAGQDKEFAHFYGEITAMDRAFGKLRHEINRLGIRENTVLWYCSDNGGLPKVGST
                     GGRGSKGQIYEGGLRVPALLEWPARFPKPRVLNTPCVTSDIYPTLLEIAGAPVPDQRP
                     LDGISIIPLLEGVMAMRPKPIGFWSHPGDGILTPSDTWMTELLQAQQEGKEINDPVRI
                     RPDAGEIKEHYPTESFPGHSAWLDWPWKLHRIEDQDGGVVCELYNLYTDPGETADVLL
                     ENPEQAEGMLAQLLDWLASVANSLNGNDYG"
     gene            complement(14325..14906)
                     /locus_tag="PKW60_00155"
     CDS             complement(14325..14906)
                     /locus_tag="PKW60_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HPJ97868.1"
                     /translation="MMRRVVIPLMLGVFAATGCIRPTVTPTIRYVLKPEIAVAQASPT
                     ERSLGIRRFRASALYRTPILYAQDHQIHPYPYAEWALNPEDMVTRAITDAIVATGRFR
                     DAGDASNVNRPELMLVGELRRFEADRDASPVVAVCEVRIELREAFGTGLLWADTLVAR
                     VPLAEDRVDALPDAMSLAVAQIANKAATEIAAH"
     gene            complement(14903..15904)
                     /locus_tag="PKW60_00160"
     CDS             complement(14903..15904)
                     /locus_tag="PKW60_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPJ97869.1"
                     /translation="MATQAQKMKVGIFLVGCLAVLAGGFLVVSGYKSGEHTNYFIQFD
                     ESILGLSRGGLVVYNGVPVGKVSDITVSETRSVRVDIEVKDAEITLSPGVEAQLVMYS
                     IATGTMTVSLSGGNPEEGKLPPGSEIPSRPSLFASTAGSIPEVVKKVSDIVDKINTGL
                     EGIEEGQIKAMLDEADNALQDAREVFSETATTLKELRIKTVEGVDDARKLIEEIRMEI
                     RPFTENTNALVKSANSTLSTINEKITPLDFSKIEGELLNTLTSIRELADAARKTTGQL
                     ETVSKTVVYEADNVEFMVRQTTDSLQETLEALRALADTLRRDPSAVVYGPAHPKGEK"
     gene            complement(15916..16704)
                     /locus_tag="PKW60_00165"
     CDS             complement(15916..16704)
                     /locus_tag="PKW60_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ97870.1"
                     /translation="MCSKILQKAVEPVIEVRNLVARYGDQVVLNDITFEVPRGEIFVI
                     LGGSGCGKTTLLKHMTGLLQPAAGHVYYSGTDITTLDEDQLAGIQQNIGIAFQSGALF
                     NSLTVGENVALPMREYTDMPEELIQAMVVLKLSLVGLASAKDQLPEELSGGMKKRAGL
                     ARAIALDPPIVYFDEPSAGLDPITASGLDDLILDLKRLLRITFIIVTHELESIKKIAD
                     QVLMLDDGKAVFQGTVHEAQTSSIQRVRQFFERKADTYIRQQHT"
     gene            complement(16694..17779)
                     /locus_tag="PKW60_00170"
     CDS             complement(16694..17779)
                     /locus_tag="PKW60_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ97871.1"
                     /translation="MSGIRVECPRDLNQDTGRALLDVVRERMRSQDGGDLVLDFTPTD
                     TMDSSGAAWLVHIAQFVRTSNADFLLEGQHGGVAEFINLIGPGLSGTEERKEKSDNLF
                     ERIGAKGFGMAEEARQMLSLFIDAVYWTFIGPIEGRGLRWGLVAEELYEMGVRAIWIN
                     SVMNALLGLIVAMLSAAQLRTVGLDIYVADLTVIAFARELAAIMTAVVVSARTGAAIA
                     AELATMKVQEEIDALRGMGLKVPQFLVAPKVLALLIAMPCLTVVAMYMGVFGGAIWGM
                     GVLGFSGEIWFRQTIGALTQGDVLQGLLKSLFFAVSIVLIGCHNGLRVRGGSRGVGLM
                     TTRAVVMDIFMIVVIDMLFAVVFYYVL"
     gene            complement(17776..19803)
                     /gene="ligA"
                     /locus_tag="PKW60_00175"
     CDS             complement(17776..19803)
                     /gene="ligA"
                     /locus_tag="PKW60_00175"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPJ97872.1"
                     /translation="MDHAEGNISPEIRKRCEQLRNELDRHNRLYYVEAKPEISDMEFD
                     ALLNELIAIETEFPELVTPDSPTQRVGGQPLEGFKTVTHTIPMLSIDNTYNEGELRAF
                     DDRVRRGLEGQEPAYVAELKIDGVSMSLRYEAGRFVQAATRGDGARGDDVTANVKTIR
                     AVPMRLQGACPDLLEVRGEVYMRRQELIRLNELREEAGEPPLANPRNTTAGTLKLLDP
                     REVARRRLEIACYDVAPLPGIELTSHWQTLHDLKSYGFPVNPFFKRCRNIDDVIAVCE
                     EWSSKRHDLDFETDGMVIKVDSAEQRRQLGATSKAPRWAIAFKYPAEIAETKLETVSL
                     QVGKTGTVTPVANLAPVHLAGTTVKRASLHNFDDIERKDIREGDIVRVQKAGEIIPQV
                     LGPVLEKRPKDARKIPVPTACPQCGSEVRQDPDGVYLRCLNPACPAQIKGRLRYFASR
                     SAMDIEGMGEKLIDQLVDKHLVRNLDDIYKLTPEQLAELERMGEKSALNLVTAIAMSK
                     KRPLSRLLNGLGIRHVGEHVAEVLAGHYGTMDALLDAPAESLCKVPEVGEVVAREVRN
                     FFETEENQALIASLRAHGLTMEEPRTATGSPQPLAGKTVVVTGTLTRYSRDAIANRIK
                     QLGGRTSSSVSKKTDFVLRGDNPGSKLTKAQQLGIPILTEDEFDDLAEPKA"
     gene            20038..21453
                     /locus_tag="PKW60_00180"
     CDS             20038..21453
                     /locus_tag="PKW60_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97873.1"
                     /translation="MPWNSLQWILVCVQAVCGAGDYPLDMDKPLRIAGVEVDRVEAPV
                     FGRITLDIALEARYSNPFRSEEIRVDVAVEPESGERFTVPAFLYQPFERRLEDTDKGP
                     AERLQPAGEPRWQARLAFSEPGRHALQVTAIDRSGRVESGPISVNIAPADVAGMARRH
                     PQDHCYFVTDRGETLYLIGANVCWAGRRGTFDYDDWLAKYAEANCNFIRVWLSPPWTT
                     FAMNTLESGYDGIALGNAWRLDYVLEQSERLGLRMMLCIDSFNILRSTEVLYGLWEDT
                     PYIQANGGPLVRSEDYFTEPAMLAAYRDRLRYLVARYGYSTSVFAWEFWNEVDIIDTY
                     RSETVAAWHADMARFLRVLDPWKHLITTSHARPYGDPAVDGLPELDFVQTHHYEAKDM
                     ARDFGLDIAQKSAAADRPHFHGEFGIDHRGRARDLDPTGVHIHNGAFSSVGQLQAGIP
                     LTWWWDAYVHPQNLYPIYGAF"
     gene            21514..>21720
                     /locus_tag="PKW60_00185"
     CDS             21514..>21720
                     /locus_tag="PKW60_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97874.1"
                     /translation="MDIATSDLRIPGDSLVQTEAASWDPAPWNQPATVRISREGDLQS
                     DLTVSRLQHGVRNHPDLHNPITFEV"
BASE COUNT         3826 a         6596 c         6774 g         4414 t
ORIGIN      
        1 ggcggattcg gctggttccc gacacaatgg tccgacacct gggataccca gcccgtgttc
       61 agcctcgatc ccaccacgcg cgtgaccatg gtcgggagtc tcatcgcgac gacggtctgg
      121 tgggtctgca cggccggctc cgaccagatg gccatccaac gctatctcgc cacccgcgat
      181 gccaaatctg cgcgccgggc atttctggtc aataacatcg ctgacgtctg cgtcaacgtc
      241 accctcattt ccctgggttt cgcgctgctg ggattcttcc gcacgcatta tcccggcctg
      301 ctcatgtccg cgctggcgga cgaaaagagt gccgattacc ttttcccgct tttcgtggtt
      361 aatttcatga aatacggtat ggctggactg gtcattgcag gcatgctggc cgcggccatg
      421 tcgtcgctct cttcggggct caacgcgtcg gccacggttt tcaacaccga tttcctgccg
      481 ctgctctttc ggaaagagtt ggatgaaaga ggcaaggtcc ggcaggggat ggcggtatcg
      541 gcggtgatcg gcgtcatcat tattctcttg gccctggtca ttgaaaatgt ccggggcaac
      601 ctgtttgaag ttacgcaaaa gaccagcaac cttcttgtct cgcccctgtt cagtcttttc
      661 tttctggcct tgtttgttaa acgagccaac accttctgcg ccatgtacgc cgccgcgtat
      721 ggattcgtgg tcgcggtcgt catcgcgttc tgggacctca ccggcgccac gcccatcagt
      781 tttcagtgga tcggcatcac atcgctgctg gcgagcgtag gtgccggcgt cgggctgagt
      841 ctgctgccct gggagcgcat gacccgcaac agccggctcg gcgcaggcat cgcagcggcc
      901 ctgcctctgg tcattctggc tggttttttc attgccggga ttgcggccag gccaggcgct
      961 gccggctgaa gatccagggc cttctctcta tctttggacg aagtctgacg tggtaccggg
     1021 ttgggcagca aggctcattg caccgcaggc gcgggcgatt ttttagggac cgcggttctg
     1081 gtgtacactg tgtgcatcta tgaacagacg ggtacatatt tgcgtcgcca tgctgctgct
     1141 tgccagggcc gccctggggc aggcgcccag ccagttgtgg accgaggcca ccatctaccg
     1201 cgacaactgg ggcgtgcctc atgtctatgc tgagaatccg cacgctctgg cgttcgcgtt
     1261 tggttatgcc caggcggaag accgcctgga gaccatcatg cgggcttacc ggcttgtcac
     1321 cggccgcgcg gccgccgttt tcgggccgga ttatgccgag tcggacgcct ttgcgctcaa
     1381 gctgggccat agcgacctgg ccgtgcatgc gctcgagagc gcatcgccgc tgacgctcga
     1441 cctctgcgaa gggttcgccc tggggatcaa tgcctggatc gcggatcatc cggcgcgcgt
     1501 gccggactgg gcggacggcg cccggccggc cgacgtgctg gcgctcatgc actgttatct
     1561 catgagtttt gcccccttcg acctgccgga gctgtttcac cgcccggcgc ccgcgtattc
     1621 gggcaacgcc tgggcgctcg cgccatcgcg tacgctgtcg ggcgcggccg tactggtgct
     1681 gaacccgcac ggatactacg acgggccgtt ccaatggtac gaggcgcatc tggccggccc
     1741 gcaggtaaat gttgcgggag cgacgctctg cggattgccc gtgattctgc agggccacaa
     1801 tgaggtcctc ggatgggcgc tcacgcccaa ctatcccgat tttgcggaca tctacacgat
     1861 gccggagggt gagatccggc tgccgcccgg cgatccaaac cggatgcagc tccaggccaa
     1921 gctcgagcgg ctcctcattg ggcggcaagt gatggaaagc cgggtctatt ttgtcggtac
     1981 gccgagcggg atgcgccagc gcagcgtgcc gttccgcttg acggcccggg gccctgtggt
     2041 gggcgagatt ggatcggagc cctgcacgtt catggcgggc gggtatgccg atttcgagac
     2101 gctggaccag atgatggcga tggcggctgc ccggaatctc ggcgagtttc gcaatgcgct
     2161 ggggatgcgg cagctgccct gtttccatgt agtctatgcc gaccgggccg gcaacctctt
     2221 ttatctctat aacgcgaaag tcggcgacaa aacccttccg cctgcgctgg cgcgtgggcc
     2281 ggagctggag cctgggagcg gcgaagggcc actcgggccg cacgcggcgc cctatgtctg
     2341 ggacgcccct gttcccgcca acaatccgca attcgcgtgg ggcgaactcg tgccgctggg
     2401 tgccttgccc acgattgtga acccgcctgc gggattcata caggcgtgcg gcacgccgcc
     2461 ctggggagcg accctcaatt ccggcattgc ccccgcatcg ataccgctct ggctcgccaa
     2521 tgaacgtgac accttccgcg cgcagcgcgc ccgccatctg ctcagtatgg gctcccggag
     2581 ctttcacgaa gcccaggcga tggtgtacga cgtgctggtg ccgttcgcca ccgccgctgt
     2641 gccgcaactc atacaatggg cggacgctcg tgagcacttc tacgagaccg cccatcccga
     2701 caccgcccac ggagccgata tccttcgctc ctggaatttc gtcgcggaga ccggcagcgc
     2761 cggcatgacc ctgttcaatg cctggtgggc ggcgtaccgg cagctcgccg gcccgcatct
     2821 gcccgacgtg atcctgtacc aggagttcgt cagtaagccg ccgcatgtgg agcggttggc
     2881 gctcgaagcg ctgtcgaacg cggcccgtga aatgcgcaac gtccatgatg cgctggatgt
     2941 gccgtggggc gacgtgcacc ggatgcgcaa gggcccgcaa gaagcgccgg tcgcgggggg
     3001 cgaggcgggt gaaccgctga tggtcatggg cgactacggc attgcacggc ggaagcttct
     3061 gccgaactac gggttcgcgt acgccatggc catccagttc ggcgatacgc cggaatcggt
     3121 aagcatcctg cccggcggta tctccgacga tccatcgtcg cggcacttcg ccgatcagct
     3181 caagctgttc tctgaacgcc gcttcaaagt gaactacttc ctcgacaagg acgtgcagcg
     3241 ctatgcagaa cgggcattcg ggaggcggcc caccctccgg cctgttggaa tgcagggcgt
     3301 gttcggcatc gaagcccgcg accccgtttc aatacacctg gaatgcagcc cgaatgcgcc
     3361 cgctcccatg cccgacggca tggcgccgtt tacgatattc gtcactccgg tcctggacag
     3421 cgtgcatgcc gccgtcacca ttgacgccgc catctatgtg ccgccgtccg tggttgaaac
     3481 ggccgggctg caacggctcg cggtgtgttt tcttgatccg gcgcacgggt ggttccccgc
     3541 gccggaacag caggtgaatc cagaaacgcg catcttccgg gcgcgtctgg aagggacacc
     3601 cacctgcgcc gtgctgggtc cggcgtcgct ccgcgcgcgg ctggaaagcc caccggcagc
     3661 gccggcacgc gccgaggaag cctccccagc gcccgtgcag gaaacaccgg cggaacccgc
     3721 gtctgcactg gtcgagactg atgagccgga ggcaccggat gcaccccccg cggagtccga
     3781 gccgcctgca ccgccgcctc tagccccagc tgccagcgcg gcgccgggtg ttcccgacgg
     3841 cgcgctgcgc tcgggtttgg cctggggcaa atcgcttgag atagccattc ccggcggtgc
     3901 gggcacggtg cgcctccagg catcggactc gcttggcgcc ttcgtgagcg cctcgacaga
     3961 gccgcccgcg cctgtgccgg agggtctgtc tccgataacg ccgttcgcag gtgtgcattg
     4021 ctctgatccc gcggtctccg tcacgctgca actcgaactc actgtcccgg ggatcacggc
     4081 agcaaacgcg agcgaggtcg aactgctggc ctaccgggcg gaatccggtt ggcaggcgtt
     4141 gtctgatcag catttcaacg cagaggctgg tgcgatgatg ggctccgatg accaatcagg
     4201 cgtttacgcc gcgtttgcac gtggagctgc gtcggcaatg cccccagctg aaatacagac
     4261 tcaggaaatg ccctccgcag cgcccgtgga gatcgccccg gctcttccag ccgcggttcc
     4321 gacggagaaa gcagcgcagg atcatgccgc agccgaacca gcgtccgagt cccgaaaacc
     4381 tgcattggcc tggggcaaga cccttgaact catgtccgag aaccacagcg cggaattcag
     4441 acttgaagca gatgcatcaa tcggcgcgtt cacaaaagtc ttgtctgaac cgcctgccac
     4501 gcttcctgaa ggccttcagg cgtacggcgc ctgcgtggcc ctgtccctct ccaaacgcga
     4561 cgtccctgtc cggatcaccg tcaccctgca tgtggggagc gaaatcccgc agggacttga
     4621 ccccgcttcg ctcgtgctct atgcgcacga agcggacacc ggctgggcac gcctcgaagg
     4681 ccagaaattc gaccctgccg cacgcacggt cagcgccccg gatcaccagc cccgcaccta
     4741 cgctctgctt gggcctaaag ggtcttgacg cttcgccttc ccgatggagc gccctgtttc
     4801 tgaggcgggc cgcttgcgcc cgcccgttgc ctgaggggct attgctgagg ccgcaggcgc
     4861 ctatcccgcg aaatagacct tgagcgcgtt cacataggcc tcggcaaacc attgccgcca
     4921 ctcaggatct tccaggcggg cgcagtcgac gtcattggcg agattcgcgg tttcgatgag
     4981 gattttggtc ggcaccatgg tgttgcgcaa taccgcgggc acaaacgacg tgccgtcgcg
     5041 gcggatttgt gcgcggatgg gggcgcttcg gtcatggcgt ttaatgcgtt tttttccgag
     5101 ttcatccatg agcaccgtgg cgaagtttcg cgacagggcc tcatcgcgcc gccgttccgc
     5161 ggcggtggaa ctggcgtaat tctgttcgcc cacttctttg tacttgtcgt aactggccag
     5221 ggtgagcgca ggcgcctcct tgtcgcggcg tcccgccgca cccggcacgt agatcatggc
     5281 gccccgcagg ctgggatgga gtttgtcgca atggatgctt gaaaacacga cttttttcgg
     5341 gtcgacacca cgcgcaacct cggcccggta aatcgaattg gccagatacc agcgcagatt
     5401 aagcgacatc ttgtcgccgt ccaggtcgcg gtgccgcgga gaggtcagca gctcttcgtt
     5461 ggtgtcgtgc acgaaacggg tgcggtctgt tggggtatat ccctgtatgc ggtctattac
     5521 ggtcaaatgc accgtggcct gcgtctggga ttcgaggatc tgcttgatcc ggcaggcaat
     5581 atcgtaattg atctcatctt catacagact gaagcggttg ttggtggtcc aggcgccgtg
     5641 gtcacgtcct ccgtgcccgg gatccagaat gacaacgacc cccgccagat ctttgcttcg
     5701 cacggcgccg cgcaggcgcc gggcttcgag caccatcgcc tgatactcgg agcgctcgga
     5761 tgagccttcc ggcatgtact gggccgaaag catgtccatc gggatcttga tcgcctggcc
     5821 gacggggatc gaccgctcat cccgaatgcc actgcgctcc atcaccacac tgcaggcggc
     5881 gaggatggtt gccgcggccg attcttgcac atccacgtcg gtgaagcgag cgaccacgtc
     5941 cgtgtacaga gcttctccgc gtttcaggcg atacagcgca taagcccccc gggcgtcgcg
     6001 gctgtattcg agctcattcg ctcccggcgc cgcgctcgcg gccagcgcgt gcaggggcgt
     6061 gtctgtcggc tcctgggccg gcgccagcgg agatgccgcc agcaatccgg gcggggcagg
     6121 ccgctccccc gtgggtttct tcatcgcatc gagaaggagg atcgatggga aaaacaggga
     6181 ttgtccctgg cgaagcttgt ccgtctccag tttgttgatt ttctggatct ctttgcaggt
     6241 gctgccttta ccagtcagcc accggcacag cgtccacagg gcctccgggc ctgaatcctc
     6301 ttcatgaagc acgtagtgag tccagccgtc tggcgtgacg acgtcgtacg ggaatacggc
     6361 cagcaggact tcacgttgag cggccgggtt caactcgtca aaggggaccg caatcgagga
     6421 cgtcatgccc tgatacgttt tccaggcgct cgaatcggcc aggtatttgt cggcgaatgc
     6481 cctgggcgcc ggtcctgccg cccggttgta ttccagcaac agcgatcggc catttcgtga
     6541 tgcggcagac acgcccggcg ccagcgggac acgcatgacg cccgcgtaca ccggtggcga
     6601 acctgtcagt gccacaagca gcaggaggag aggtaaattc ttgacgaagc gaggccgacg
     6661 gagccgttgt gtcattgtat gtttcatggt caggtcacac cggtgagacg ggcccgaaaa
     6721 agcccgtttt ggtgaagagt gcggggacgc tagtgtcccg ggtacagttc gcgaagacgt
     6781 tgccagacat attctttgat ggcccgagtg gttgctgcat ccattttccc aaaacacacg
     6841 ccgacgataa aacggcagct atcgtgcggc ggcacaagac gttcgatgtg tgtcacgcgc
     6901 ccatggatgg tccggacggt gtcgagtggg atggaaatcg tcatttcgac actgctgttc
     6961 aagtccaggg gcgcatcggt ttccagcagg gcaccgcctc cgctgagatt gagcagtgcg
     7021 gcgtcgtatt gccggatgag ctccgcttct ttgatctgta cggtcaagtc cgtggagacc
     7081 cggcagttct ggcggtgcag tatccgctcc atggcggccg ggcgctcaac gatcacgcaa
     7141 ccggcatgtt cctgcggccc agccacgaca agagacaggt aggaattgaa gccttcttca
     7201 tcgtggaatt ggagttgaag ctgcataccg ggcgtggggt aatcggtacc ggggaaggtc
     7261 agtgtgaggc ggtcttcgga ggcgtcaagg atgcgcccca ggaagtgttg ccccatcaaa
     7321 aagacaaggc attctctccc tttgctgagg tcgggcgtat cttcgttccg aactgcctcg
     7381 tctgtcattc ggcgcactcc ctttgaactc ggtcgcattc tagcacaggc ttttcaagac
     7441 gtcaaaacct gtgaatagtg tacggggtgt caagaccttc tttttaaaaa tggctctatt
     7501 ccgggattgg ctcggccggg gccgcttttg catcaaaacg ggctgatttt gtaagaatct
     7561 gcactcacta gcctgcgaaa gtcccgcgcg cgtccggacg tccgtctttt tgatgccgac
     7621 gaggacgtcg gcgcaccgaa agtcccgcgc gcgcccggat gtccgtcttt ctgatgccga
     7681 cgaggacgtc ggcgcaccga aagtcccgcg tgcgtccgga cgtccgtctt tttgatgccg
     7741 acgaggacgc cggcgcaccg aaagtcccgt gcgcgcgcgg acgtccgtct ttctgatgcc
     7801 gacgaggacg tcggcgcacc gaaaatcccg tgcgcgtccg gacgtccgtc tttctgatgc
     7861 cgacgaggac gtcggcgcac caaaagtccc gtgcgcgtcc ggacgtccgt ctttttgatg
     7921 ccgacgagga cgtcggcgca ccgaaagtcc cgcgcgcgcn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnt ttttgatgcc gacgaggacg tcggcgcacc gaaagtcccg
     8101 cgcgcgcccg gatgtccgtc tttctgatgc cgacgaggac gtcggcgcac cgaaagtccc
     8161 gcgtgcgtcc ggacgtccgt ttttctgatg ccgacgagga cgtcggcgca ccgaaagtcc
     8221 cgcgtgcgct catggctcag ggaaaaatgc gagctgggaa cgagcgcaag ccaagtcaaa
     8281 tcagtacatt gaggcagaat gttgcaggag atggtacact gaaggcagaa tagcgcacgc
     8341 aagcagaatc gagtctgggg aatggttgtt ttggtgcact cgattcgaag cgcgtcgacc
     8401 tgcacggtca ggggagagcc tcggtcgcgt aaagcgaggg agaatgatgc tcgagcggat
     8461 gccccaggaa gtctcgtatc tttacattct aaaactgtcg gaacttcttg tcttaacggg
     8521 tgttgcgata gcgatcgcgg tggcgggcta cgccgtgttc cgcgtgtggt accagcgcga
     8581 caaagtcccc ccgttgtggc tcattgtgaa tgcgggcatt ctggcactgg cgctgtacct
     8641 gattgcggcg atccccatct gggtgcgtgc cgatgcctac cgcaaattga cggttgtctt
     8701 gtacagcctg gtcatcgggg tcattcccgg cgcctatttc agctattcgt ttctttccgc
     8761 gatggcgttg cggagtgttc ggcgcatcac ctggctggac acgtcgctgc ccggcgtcga
     8821 cgaatttctt caagcccggg agcttgcggc gcagggtgat atcgatggcg cggtggcgat
     8881 gtacgagaat tacccggaaa aacagggtca tgcgctgttc gctgcggccc acctgctcga
     8941 gatgaacggg cgttttcagg aagccgctga gatgttccaa aaatgcgccg aaatctcggg
     9001 cgagcagctt cggccgtggt cggaggccct gttccgctgg gcgaggctgt gcgaagtcaa
     9061 tctcgatcgt atcgacaaaa gcattgaact ttacgagcag ctggtggaac gtgccccatc
     9121 gacggagctc gggcgcctgg cgtcgacaaa actggttcgg ctgcgaacga aaaccggagc
     9181 acgtgcctca gcacgcgcct cccgggctag cgctactgcg ccgagccgca aaaaagctgc
     9241 cagacaacgc agcaaaaagg gcaccgctca aaaatcgaag aagtgaaggc gcgcttaacc
     9301 ccctcctgag gctgtgtttt cacagtgccg cccggtaatg ccgcagttca tttcgccgcg
     9361 cgactgggtt tgagagaagg ccctggcgcg gcatttccta tgctgcccgg ccgctattcg
     9421 gtttcgtgcg gcacggctcc gtggcattcg ggttcatgtt gcaggtggta ggccagctcc
     9481 gcgagaccga ccacaatgtg ttcatggcgc accacgacgc cgctgggaac ggtcagtgtt
     9541 gttgggccga agttccagat ccccgcaatg ccggcgcgga ccagccggtc cgcgagcgcc
     9601 tggcaggctt cggcgggtac agccaaaatg gccaggcgga cgttgttgcg ttcaatgacg
     9661 gtctcgagtt ctgtcgtgtt gtagacaaaa tgcgtgccga ttgtagtgcc gacttttccc
     9721 ggatcctggt caaacacgcc aacaatgcgc agaccgtact ttacgaagcc gcggtatgcg
     9781 gccagggcgc cgcccagacg gccggccccc accagaatgg cccggtggat cgtgtcgaag
     9841 cccagcgcct gccggatggc gtccaccacg tccgccgccc ggtagcccag gcgcggcacc
     9901 ccgcgtaccc caatcgccgc gaggtctttg cgcacctggg tgtcatccat ggtaagcagc
     9961 tgggcgagtt gctggctgga gacgcgtgtg gtctcatcga ggtctacgcg ttcccccaga
    10021 aaatgatagt agtggaccag ccgttcgaga tgtgcatggc caagttcata tttcgccttt
    10081 gaccgcccca tactgctcga atactcctga tgccggaagt cctcggctac ttgcgtggcc
    10141 tggcccgaac gcggccgcct tgtccatgtc ccggcacgcg gccgatggcc tcaagccacg
    10201 cgggcatgtc cgcgtggtac gacgtcgcgg tctcgtcaaa gcaggcctta gcggggtaga
    10261 tctcatacaa gcggcggtag cggggcggcg tgatgttcgg catgatgaca ttcgctcccc
    10321 gtgccagccc cagctcgcgt cccccggcgc ggttcagggt tgccagcgcc gtggtgctgg
    10381 gaatattcgc ttcgggacag acgagccgcg tcaacgcaac cactttgtat gtcatcaact
    10441 ccgtattcgg aacctgctcg ccgtcgacgc atgggcggct ccagtcgccc tggccgagcg
    10501 gcgtctccgg atgttcaata tacggccctg ccccgatcat gtccagatcg agcgtgcgga
    10561 aggtgtggat gtcgtgggcg aggctttcat agctctgccc ggggatgcct accatcacgc
    10621 cgctgccgat ctcgtacccc agctgccgga gttgcgcgag aatggcaaag cggtccgaca
    10681 cttgcccgcg ggcgtcgggg tggatcgtgc ggtagagggc gcggtcggat gtttcgaagc
    10741 ggagcaggta acggtccgca ccggcttcac gnnnnnnnnn nccttcagat cctcgaaggg
    10801 gcgctcgccc agactcaagg ttaccgccag agcagtctcg gttttgatgc ggcggacgat
    10861 cccggcgagc cagtccgcgt cgattccata atcctcgccc gcctgcatga cgactgtgcc
    10921 gtacccgtac tgcacggcgt cggcggcaca gctcaggatc tcgtcttcgg acatccggta
    10981 gcgcgcgact tcacggttgc cggagcgcag gccgcagtac ccgcacgcgc ggatgcaatg
    11041 atttgaaatt tcaataagtc cccggagatg gacctcatcg ccgacggccg cccggcgcac
    11101 cctgtcagcc agcgcccaga gttcttccag gcgcgcgctg tcctcctcac gaagccaggc
    11161 ctggatgccg gcttcgctga gttcactgtt gctggggtta gataatctcg tcatcatgat
    11221 gcatacgaac cctgtgccct acaggtggtc ggctgcgggc atggtctccg gtacggccgc
    11281 cccggcttgg acgccgcagt ctgttttcgc ggcgcgatac gcctccaggg cttcgggaaa
    11341 cggctccagg gcgcgctcga agatgcccag gctgtacgaa atggtcagcc cgtaattcgt
    11401 tatgggcacc cccgcctcac ggcatttgag gatccggttc aagatcgcac gtcggtttgc
    11461 cgtgcacgcg ccgcagtgaa ttacgagctg gtacggggcg atctcttccg ggaaatcgtg
    11521 tccctgcacc cattcaaatt cgagcgggcc gcccacatac tgcgtgagcc agcggggaat
    11581 cttgacgcgg ccgatgtctt cctcgatggg gtggtggctg caatgttccg caatcagaac
    11641 acggtcgccg ggttgcagag attcgatggc catcgcaccg agcgtctgct gaacgaggtc
    11701 gccccggaac cgcgcgaaga gtatcgaaaa actggtcagc aggatctcgc gcggagtgtc
    11761 gcgggccact tccctgaacg cctgcgaatc cgtcaccacc agtttgggcg gcacgctcag
    11821 gcggcgcagc gcctctccca gctggcggtc ttttaccacc atgcagaagg cgtcgccgtc
    11881 gagcaggtcg cggatggatt gcacctgcgg catgatgagc cgccctttcg gcgcctcttt
    11941 gtcgatcggc accaccagga ctgccatctc gcccgggcct accaggtccg ccaggatagc
    12001 cgggttattc acaaactcgt cgggagcacg gttcaggagc gcctgccgga aatcggaaat
    12061 gccggcgccg gttgccgcgg ccgtcagtac cacgggcacg ccgcgctccc gcagggtcgc
    12121 gagcaaaccc ggttcgggac ggcccaaatc cattttgttg aacacgacaa tcgcgggtat
    12181 ctcgcggcga gccaattgat cgaggatctc atcttcgaag gtgccccagg cgccgggctc
    12241 cgtcaccagc acgcccagat ccgtccgctc gaagacctgg cgcgtgcgct gggtgcgcaa
    12301 ctcgcccagg tcgccctcgt cgtcgatgcc cgcggtgtcg atgaacaata ctggccccaa
    12361 gggcagcaat tccatgggct tttcgaccgg atccgtcgta gtgccggcat gatgcgacac
    12421 gagcgagacc tgctggcgcg tgatcgcgtt gagcaggctc gattttccga cattgcggcg
    12481 gccgaacagg ccgatgtgca gtctgaatga ttttggtgcg cgctgcatgg gtctagaatc
    12541 accaggttac ctgaagcggg gcttctgtcg catgcatcgc cacgaaccgg cgttgcatgg
    12601 gctccacaac actatgatac gccaacgcag cgggtaattg catgattccc gccgggacag
    12661 cgggggagaa ggcccggatg agcggtctgc ttgtgataca gggcatcgcg aatcgtcgta
    12721 acacagggat tacatcgagc actacgccgg ttcgctgcgc gtttcgtgat cagccgtaat
    12781 cgttgccgtt caagctgttg gcgacgctgg cgagccagtc aagcagttgt gcgagcatgc
    12841 cttccgcctg ttccgggttc tccagcaaga cgtcggccgt ttctccgggg tccgtgtata
    12901 aattgtagag ttcacagacc acgccgccgt cctggtcttc gatgcggtgg agcttccagg
    12961 gccagtcaag ccaggcgctg tggccgggaa acgattccgt ggggtagtgt tctttgatct
    13021 cgccggcgtc ggggcggatc cggaccggat cgttgatttc ctttccctcc tgttgcgcct
    13081 gaaggagttc cgtcatccag gtgtcggacg gggtcaggat cccgtcaccg ggatgagacc
    13141 agaacccaat gggtttcggg cgcatggcca tgacgccctc gagcagcggt atgatgctga
    13201 tcccgtccag cggccgctgg tcggggacgg gcgccccggc gatttcgagc agcgtggggt
    13261 agatgtcgga ggtcacgcag ggggtgttga gtacgcgagg tttcgggaac ctggcgggcc
    13321 attcgagcag ggccgggaca cgcaagccgc cttcgtagat ctggcctttg ctgccgcgtc
    13381 cgccggtgct gccaaccttg ggcaatccgc cattgtcgct gcaataccac aagaccgtgt
    13441 tctcccggat gcccaggcgg ttgatctcgt ggcgcagttt gccgaaggcg cgatccatgg
    13501 ccgtgatctc accgtaaaaa tgagcgaact ctttgtcctg ccccgcgtag agttccaggt
    13561 cactgtcgag cgctttgtgc gggttgtgcg gcgagccgaa ccagactact gcaagaaagg
    13621 gagccgggct gtctacgtgt ttctgcataa acgcgatagc cgcgtctacg gtgaccatgg
    13681 agctttcccc ctggaattgg acggcctgcc cttcgcggct caggatgggg tcattatcaa
    13741 agaaattcgg cgcggagagc cattcgtcga agccgcttgc accggggttc acagggctgc
    13801 cgcgctggac ggagccgagg tgccattttc caaagtgacc cgtgatatag ccggctgctt
    13861 tgagggcttc cgcgacggtg atttcctggg ggcgcaggga gtggccccat cggaaacatc
    13921 ccattcggtt cggtgtgcgg ccggtcatga cgctggcgcg cgtgggcgaa cacacgggtg
    13981 cggccgcata aaaccggtca aaacggatgc cctccgcggc catggcgtca aaatgaggcg
    14041 tctggagcac cggatgcccg ttgtaggcca tgtcgcccca gccctgatcg tcggccatgc
    14101 acaagacgat gttgggccgg cgtccatcag cgctggcgcg ccgcgtgaac cagcccaacc
    14161 cggccgtcga tgctcccgcg gccagcaatc cgagaaattc tctgcgccgc atacccgtga
    14221 tcctcctcaa ttcagtgtcg tcgacaccat ccccgcatca aatgtatgcg tctctatccg
    14281 cgaccattcc agaatcgatg aaattgagaa tgatatgttg ccactcaatg ggcagcgatt
    14341 tccgtggcgg ctttgttggc gatttgagcc acggcgagac tcatggcatc gggcagcgca
    14401 tctacccgat cttccgcgag cggcacgcgg gcgaccaagg tatcggccca gagcagaccg
    14461 gttccaaacg cttcacgcaa ttcgatgcgc acttcgcaga ccgccacaac cgggctggca
    14521 tcccggtcgg cttcaaatcg ccgcaattca ccgaccagca tcagctcggg gcggttcaca
    14581 tttgaggcgt cgccggcatc ccgaaacctt ccggttgcaa cgatggcatc ggtgatggcc
    14641 cgggtgacca tgtcttcggg gttcagcgcc cattcagcat acggataggg gtggatctga
    14701 tggtcctggg cgtagaggat gggcgtgcgg tacagagcgg aggcgcgaaa tcgccggata
    14761 cccagcgaac gttcggttgg ggaagcctgt gcgaccgcga tttcgggttt caggacatag
    14821 cggatcgttg gcgttactgt aggccggatg caaccggtgg ctgcaaaaac gcccagcatc
    14881 agggggatca ctacgcggcg catcatttct ctccctttgg atgtgcggga ccgtagacaa
    14941 cggcagaggg gtcccgtcgc agcgtgtcag cgagggcacg gagggcctcg agggtttcct
    15001 gaagtgaatc cgtggtctgg cgcaccatga attcgacgtt atcggcttcg tagacgacgg
    15061 ttttagaaac ggtttcgagc tgccccgtgg ttttccgggc ggcatcggcc agttcgcgga
    15121 tgctcgtgag ggtgttgagc agctcgccct cgattttgga gaaatccagg ggggtaattt
    15181 tctcattgat cgtgctcaag gtggagttgg cagatttgac gagcgcgttg gtgttctccg
    15241 tgaaaggccg gatctccatc cggatctctt cgatgagctt gcgcgcgtcg tcaacgcctt
    15301 cgacagtttt tatccggagc tccttgagcg tggtggcggt ttccgagaaa acctcgcgcg
    15361 cgtcctggag tgcgttatcg gcttcatcca gcatcgcttt gatttgcccc tcttcgatgc
    15421 cttccaatcc cgtattgatc ttgtccacga tatcgctgac ttttttgacg acttccggga
    15481 tgctgcccgc ggtagatgcg aacaaggaag gccgggacgg gatttccgaa ccgggcggta
    15541 gttttccttc ttccgggttg cccccagaca gcgagacggt catggtgccg gtcgcgatac
    15601 tgtacatgac cagctgagct tccacgccgg ggctcagggt gatttcagca tctttcacct
    15661 cgatatccac gcgcacgctg cgggtttccg acaccgtgat gtccgacact ttgcccacgg
    15721 gcacgccgtt gtagacgacg agtccgccgc ggctgagtcc caggatggat tcgtcaaact
    15781 ggatgaaata gttggtgtgt tcgccgctct tatatcccga caccacgagg aatcctcccg
    15841 ccagcacggc caggcatcct acgaggaaga ttcccacctt cattttttgt gcttgggttg
    15901 ccacgttatc ctctcctagg tgtgctgctg ccggatatag gtgtcggctt ttcgttcgaa
    15961 gaattgccgc acgcgctgga ttgagcttgt ttgcgcttcg tgcaccgttc cctggaacac
    16021 ggctttgccg tcatccagca tcaggacctg gtcagcaatc ttcttgatgg actccagctc
    16081 atgggtcacg ataatgaacg taatccgcag caggcgtttc agatccagta tgagatcgtc
    16141 gaggcccgaa gccgtgatgg gatccaggcc ggcggaaggc tcgtcaaaat acacgatggg
    16201 gggatcgagt gcaatggccc gtgccagtcc ggcccgtttt ttcatccctc cagagagttc
    16261 ttccgggagt tgatcctttg ccgaggccag cccgaccagg ctcagtttca gcacgaccat
    16321 ggcctgaatc aattcctccg gcatgtcggt gtactcgcgc atggggagtg caacattttc
    16381 ccccacggtc aacgagttga acagggcgcc cgattggaac gcgatgccga tattctgctg
    16441 gattccggcg agctggtctt cgtcgagcgt cgtgatgtcg gtgccggagt aatagacatg
    16501 gccggccgcc ggttgcagca ggccggtcat gtgcttgagg agcgtcgttt ttccgcaccc
    16561 gctgcccccg aggatgacaa agatttcgcc cctggggact tcaaatgtga tgtcgttgag
    16621 caccacttga tcgccatagc gcgcaacgag atttcgcacc tcgattacgg gttcaaccgc
    16681 cttttgcagg attttagagc acataataga acaccaccgc gaagagcatg tcgatcacga
    16741 cgatcataaa gatgtccatc accacggccc gggttgtcat caaaccgacg ccgcgggagc
    16801 cgcctcgcac gcgcaggccg ttatggcacc caatgagcac gatggagacg gcaaaaaaga
    16861 gcgatttaag caagccctgc agcacatcgc cttgcgtgag ggctccgatg gtctggcgaa
    16921 accaaatttc gcccgaaaaa cccaggacgc ccatgcccca gatcgcgcct ccaaatacgc
    16981 ccatgtacat cgcaacgacc gtaaggcagg gcatggcaat aagcagcgca agcactttgg
    17041 gggccacaag gaattgcggc actttcagcc ccatgccgcg caaggcatcg atctcctcct
    17101 gcaccttcat ggttgccagt tccgcggcaa tggccgcgcc ggtccgcgct gaaacgacca
    17161 cggcggtcat gattgccgcc agttcgcggg caaacgcgat caccgtcaag tccgcgacgt
    17221 agatgtcgag cccaaccgtg cgcaactgag cggctgaaag catcgccacg atcaggccga
    17281 gcaacgcgtt catgacgctg ttaatccaga tggcgcggac ccccatctcg taaagctctt
    17341 ccgcgacgag tccccaacgc aggccccgcc cttcaatggg cccgatgaac gtccagtaca
    17401 cggcgtcaat gaaaaggcta agcatctgcc gcgcctcttc cgccatgccg aagccttttg
    17461 cgccgatccg ctcgaaaagg ttgtcgcttt tttcttttcg ctcttcggta ccgctcaggc
    17521 cgggaccgat caggttgatg aattcggcca cgccgccatg ctgtccttcg agaaggaagt
    17581 cggcgttaga ggtgcggacg aactgggcga tatgcaccag ccaggccgcg ccggagctgt
    17641 ccatcgtgtc cgttggcgtg aaatcaagca cgagatcgcc gccgtcctga cttcgcatgc
    17701 gctcgcgtac gacgtcgagg agggcgcggc cggtatcctg gttcagatct ctgggacatt
    17761 cgacgcggat tccgctcatg ctttcggctc cgccaggtcg tcaaattcgt cctcggtcag
    17821 gatggggatg ccgagctgct gcgctttggt gagtttgctc cccgggttgt cgccccgcag
    17881 cacgaaatct gtctttttgc tcacgctcga acttgtcctg ccgcccagtt gtttgatccg
    17941 gtttgcgatg gcgtcacgcg aataccgcgt caacgtcccc gtgacgacca cggtcttgcc
    18001 tgccagaggt tgaggactgc ccgtggcggt gcgcggctct tccatggtca gtccgtgggc
    18061 gcgcagcgac gcaataagcg cctggttctc ttcggtttcg aaaaaattac gcacttccct
    18121 ggctacgacc tctcccacct cggggacttt gcacagggac tctgccgggg cgtccaggag
    18181 ggcgtccatg gtgccataat gtcccgccag tacctccgcg acatgctcgc ccacgtggcg
    18241 aatgcccaaa ccgttgagca ggcggctcag ggggcgtttt ttactcattg caatggccgt
    18301 aaccaggttc agggccgatt tttcacccat gcgttcaagt tccgcgagct gttcgggcgt
    18361 cagcttatag atgtcatcca ggtttcgcac gaggtgttta tccacaagct ggtcgatgag
    18421 tttctcgccc atcccttcta tgtccatggc ggagcggctg gcaaagtatc tgagccggcc
    18481 cttgatctga gccggacacg cggggttcag gcaccggaga tacactccgt cggggtcctg
    18541 gcgtacttcg ctgccgcact gcgggcaggc cgtcggcacg gggatcttcc gggcgtcttt
    18601 cgggcgtttt tcgaggacgg ggcccagcac ctgggggatg atctcgccgg ccttctgcac
    18661 ccggacgata tcgccttcgc ggatgtcctt gcgttcaatg tcgtcgaaat tgtgcaggct
    18721 ggcgcgtttg accgtggttc cggccagatg gacgggggcg agattcgcga cgggggtcac
    18781 ggtgccggtt ttgcccacct gtaacgacac cgtttcaagc tttgtctccg cgatctcggc
    18841 cggatatttg aacgcgatgg cccagcgggg tgcttttgac gtcgcgccca gctgccgccg
    18901 ctgctccgcc gaatccacct tgatgaccat tccgtcggtc tcaaaatcca ggtcatggcg
    18961 tttcgacgac cattcttcgc agacggcgat tacgtcgtca atgttccggc accgcttgaa
    19021 aaatgggttc acgggaaacc cataggattt gagatcgtgc agggtctgcc agtgggatgt
    19081 gagctctatc cccgggagcg gcgccacatc gtaacaggca atttccaggc ggcgtctggc
    19141 aacttcgcgc gggtcaagca gtttcagggt gcccgcggtg gtatttcgcg ggttcgccag
    19201 tggcggctcc ccggcctctt cgcgcaactc attcaggcgg atgagttcct gccgccgcat
    19261 gtagacctcg ccgcggactt cgagcagatc cgggcaggcc ccctggagac gcatcggcac
    19321 cgcgcggatg gtctttacgt tcgcagtgac gtcgtcgccc cgggcgccat cgccgcgcgt
    19381 ggcagcctgc acgaaccggc ctgcttcgta gcgcagcgac atcgacacgc catcgatctt
    19441 cagctccgcc acgtaggccg gctcctgccc ttcgagccca cggcggaccc ggtcgtcgaa
    19501 ggcgcgcaac tctccctcgt tgtaggtgtt gtcgatggag agcatgggga ttgtgtgcgt
    19561 tacggtcttg aagccttcaa gcggctggcc gccgacccgc tgcgtcggcg agtcgggcgt
    19621 taccagttcg gggaattcgg tctcgatggc gatgagctcg ttcaggaggg catcgaattc
    19681 catatcgctg atttccggct ttgcctctac gtaatagagg cggttatgcc ggtccagctc
    19741 gttccggagt tgctcgcacc gcttgcggat ctccggagat atgttccctt ccgcgtggtc
    19801 catgtgcggc attatagaca atttttgtat gcaggcctac atttcaggac acgtacaggc
    19861 cgcggggagg ggagcagcgc cacaatcccg tttgattcca catacactgc ccgttccggg
    19921 ggaattcgca tgtacggccg gactgcaggt agtatgattt caaaacgaga caagggctgt
    19981 gcccgcggtg caacaaggcg tcgttttcga tgggctggat aggagaggtc tctgtttatg
    20041 ccatggaata gcctccaatg gattctggtc tgtgttcagg cggtttgcgg ggcaggggac
    20101 taccctctgg acatggacaa gccgctccgc attgcggggg tggaagtgga tcgggtcgag
    20161 gcgccggttt tcgggcggat cacgcttgat atcgcgcttg aggcccggta cagcaacccg
    20221 tttcgcagcg aagagatacg agttgacgtg gcggtagaac cggagtccgg ggaacggttt
    20281 acggtccccg catttctgta tcagccgttt gagcggcgcc tcgaggatac ggacaaaggg
    20341 cccgcggaac gcctccaacc ggcgggcgag ccccgctggc aggcacgcct ggctttttca
    20401 gaaccaggac gccatgccct gcaagttacc gcgatcgacc ggtcgggcag ggtcgaatcg
    20461 ggacccatct cggtgaacat tgcgcccgct gatgtcgcgg gaatggcgcg caggcatccg
    20521 caggaccact gttatttcgt cacggaccgg ggcgagacgc tctatttgat tggggcgaac
    20581 gtgtgctggg cggggcggcg cggcacgttt gattacgacg actggctggc gaagtatgcg
    20641 gaggccaact gcaacttcat ccgcgtgtgg ctgagtccgc cctggaccac cttcgcgatg
    20701 aacaccctgg aaagcgggta cgatggcatt gcgctgggca acgcgtggcg ccttgattac
    20761 gtgctcgagc aatccgagcg gctgggcctt cggatgatgc tctgcattga cagtttcaac
    20821 atcctgcgct ccacggaagt gctctatgga ctctgggaag acacgcctta cattcaggcc
    20881 aacggagggc cgcttgtgcg ctcggaagac tattttacgg agccggcgat gctggccgcc
    20941 taccgcgacc ggctccgtta tctcgttgcc cggtacgggt acagcacgag tgtttttgcg
    21001 tgggagttct ggaacgaggt ggacatcatc gacacctacc gcagcgagac cgtggcggcg
    21061 tggcatgcgg atatggcgcg gtttctgcgc gtgctcgacc cctggaagca cctgatcacc
    21121 accagccatg cacggcccta cggcgacccg gcggttgacg gcctgccgga gctcgatttc
    21181 gtgcaaacgc atcattatga ggcaaaggat atggcccgtg atttcgggct ggatattgcg
    21241 cagaagagcg ccgcagcgga ccggccgcat tttcatgggg agtttggcat cgatcaccgg
    21301 ggccgggcac gtgacctgga cccgacgggc gtacatattc acaatggcgc gttcagcagt
    21361 gtggggcagc ttcaggcggg catcccgctg acgtggtggt gggacgcgta cgtgcatccg
    21421 cagaacctgt atccgattta cggggcgttt tagcggtgga tcgaggggtt tgatttcgtt
    21481 cagcagcatg tccgcccact ggaggccgaa attttggata tcgccacgtc ggatctgcgg
    21541 attcccggcg acagcctggt ccagacggag gcggcatcgt gggacccggc tccctggaat
    21601 cagccagcta ccgtgcgcat ttcgcgggaa ggcgatctcc aatccgatct gacggtgtcg
    21661 agactgcagc acggggtgcg caaccacccg gatctgcaca atccgatcac cttcgaggtc
//
LOCUS       DAOPBQ010000003        21273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_2611_length_21273_cov_4.589217, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000003 DAOPBQ010000000
VERSION     DAOPBQ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 21273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21273
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2611_length_21273_cov_4.589217"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PKW60_00190"
     CDS             <1..596
                     /locus_tag="PKW60_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPJ97875.1"
                     /translation="IRVINFLTSWPILVVLAGIVALIVVYWVWQRNPVNRLLADRLKL
                     RVPLLGRISQKYAVAQMTSSLSMMLRSGLSMMVTLDLARSAIHNQAVAQVLQKVRSSV
                     EQGSGIEEPLRAEPKVIPPVVTDMLVTGEEAGQLDRVAAHIADTYEEEVQIDIGTLTD
                     LLPVILVIILGIAVLMIVLAFFLPLISSIDQLTSATS"
     gene            745..2274
                     /locus_tag="PKW60_00195"
     CDS             745..2274
                     /locus_tag="PKW60_00195"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HPJ97876.1"
                     /translation="MSIATLLEERAQQHGSRTFLICEDREYSFETMQEHSVRVAVNLA
                     GRGVQRDDKIVLLMGNCLEYLYVFLGAGRIGAVVVPVNPTLKAEEITHIANNSEAETL
                     ITAPELAPMIPQLRAALPRIKRYFVLGESTEDAESFSELLAPVASVPPILAGPDDTAA
                     LIYTSGTTGAPKGVILTHRNYLSNALMLVKSQTVVEDDRFFCVLPLFHVNAQVVTILT
                     PLMGGASVLLMKKFNPFAILPMIQKYRPTIMSAVPTIYNVMCRLAKAQEHEYDLSSIR
                     SFATGAAPMPEETYHEALRALKRPIIGGYGLTEATCASAAADARDPIKWNSVGSPLPY
                     TIIRIVGEDGKDVPFGEIGEILIAGPTVMKGYYKNPDATAEVLRDGWLHTGDLGQFDR
                     DGYLYIVGRVKDMIIRGGQNIYPQQIEDVISRLPGVEECCVVGVEEARWGQEVLAVVK
                     RTENINLAESSVIQHRRQYLASYKCPAFVRFVDELPKTPTGKIKKVEVAASFANIAKN
                     A"
     gene            2312..2740
                     /locus_tag="PKW60_00200"
     CDS             2312..2740
                     /locus_tag="PKW60_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97877.1"
                     /translation="MMHKRTLGILLCAVVLAAGLNGCATGKAHVSSEAAIRETLAAWK
                     AGLESHNLDRIMSVVSEEFVSGDGGSKADFRSYVARLIDDGTLSGAKMNIETARVTIE
                     KDMATVENVVLSGDRGSAQLEMDLKKESGGAWRIVGLEAY"
     gene            2824..2976
                     /locus_tag="PKW60_00205"
     CDS             2824..2976
                     /locus_tag="PKW60_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97878.1"
                     /translation="MKVQDFAYQVAIRTIELLEETQHYKIPENLRKEVGQKILGEVDK
                     LLQKSN"
     gene            3184..6147
                     /gene="uvrA"
                     /locus_tag="PKW60_00210"
     CDS             3184..6147
                     /gene="uvrA"
                     /locus_tag="PKW60_00210"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPJ97879.1"
                     /translation="MLEKAKSPGKSRKTAPKRKPVLSKNRKASADGLPAAHATSRDSI
                     VIKGAREHNLKNLSLAIPRDKLVVITGLSGSGKSSLAFDTIYAEGQRRYVESLSSYAR
                     QFLEQMEKPDIDSIEGLSPAISIEQKTTHRNPRSTVGTVTEIYDYLRLLFARIGTPHC
                     PDCGKPIQSQTPQTIVDGILALPEGTRVQLMAPVVRQRKGTYQKTFENIKRRGYLRVR
                     VDGEFRTVDEEIELERYVKHDIDVVVDRLLVKPGIGRRLTDSVEACLELGEGIIRIWR
                     ETPDKTVSEVLQSEHFACVDCGLSFEELSPRMFSFNNPHGACPLCTGLGEVIEVEPDL
                     VVPDPSLSIAKGAVRAWSMRRVLDGMYRHALRCVCRHYGQDPATPWEELPEDFRQIVL
                     YGSGDEVIDFTYTGRRNTFELQRPFEGVIPNLTRRYHDTESNRAREMISEFMASRPCP
                     DCGGARLRPESRSVTVGGKTIMDVVALSVRHALTFFEKLSLNKTQRLIAERVMKEIRE
                     RLSFLVSVGLDYLSLDRNAGSLSGGESQRIRLATQIGSGLMGVLYILDEPSIGLHQRD
                     NKRLIATLERMRDLGNTVIVVEHDEETIRRADHVIDLGPGAGVHGGQVVAQGTPREVA
                     ANPDSLTGQYLSGRFRIRVPETRRKSNGKALKVFGAAHNNLKAIDVEIPLGVLTCVTG
                     VSGSGKSSLINETLYPAIARGLYGSLRARPGRHERIEGLEHLDKVIDIDQSPIGRTPR
                     SNPATYTGLFTPIRELFSKVPEARARGYKPGRFSFNVKGGRCEACQGDGVLQIEMHFL
                     PDVYVPCDECRGKRYNRDTLEILYRGRSIADVLNMTVEEGCDFFRNVQAIYSKLSTLF
                     DVGLGYIKLGQPATTLSGGEAQRVKLATELSRRQTGKTFYILDEPTTGLHAVDVDKLL
                     QVLHRLVEHGNTVVVIEHNLDVIKTADWIIDLGPEGGDQGGKVVAAGEPETIAACAAS
                     YTGLHLQPLFRAKAR"
     gene            complement(6225..7052)
                     /locus_tag="PKW60_00215"
     CDS             complement(6225..7052)
                     /locus_tag="PKW60_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPJ97880.1"
                     /translation="MNDRRALIIGYGNPGRQDDGLGPAIANTVASWALSNVTTDDPYQ
                     LNIEDAAALAEHELVVFVDASVDAPEPYALTRIAPAAEITFTSHSISPESVLALCVDH
                     FHAAPEAYVLAVRGYAFEFEEGLTPRARDNMTSALACIQALIRPDKETSMDSKKTVLT
                     IDDDPDIRATLRIVLEAEGFSVGEASNGEEGLKAVERVQPDAIIVDLMMENVDSGSTL
                     AQKLKEIGYAGPVYMLSSAGDTVRYNLDARELGLAGIFQKPIDPKTLVATLKAKLKV"
     gene            complement(7049..8509)
                     /locus_tag="PKW60_00220"
     CDS             complement(7049..8509)
                     /locus_tag="PKW60_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HPJ97881.1"
                     /translation="MAKTAENGKRKIVINPVTRIEGHGKVTIVMDENNNVEQARFHII
                     EFRGFERFAKGRFYWEAPVMVQRLCGICPVSHHLAGAKATDMLVGVDKIPVTAEKMRR
                     LMHYGQVYQSHALHFFHLASPDLLFGFDAPVEKRNVIGVIEAYPEVAKKAVLMRKFGQ
                     EVIKATGGKKVHPTGAIPGGINKNLTIEERDALKKDADTMVEWAIEAVELCKKLTKEN
                     LEFLLNFGSFESNHVSIVRDDGCMDLYDGTLRAIDADGNKIFDKVQPAEYADYIGEEV
                     RPWTYMKFPHIRSLGKEKGWYRVGPLSRVNCCDFIDTELAEKARQEFMALGNGKPVHA
                     SMAYHWARCIEILHSAEKIRELLDDPDLQGTELVAEGKKREKGYAWIEAPRGTLFHHY
                     EIDTETDQITLANLIVSTTSNNEPMNRTVQVIADQHLKGKGESEITEGLLNHIEVGIR
                     AYDPCLSCATHALGKMRLHVELVDAAGTLIDEKFRG"
     gene            complement(8530..9072)
                     /locus_tag="PKW60_00225"
     CDS             complement(8530..9072)
                     /locus_tag="PKW60_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HPJ97882.1"
                     /translation="MAKPLVATAPFTGCFGCHMSLLDIDERILDLVELVEFHKSPIND
                     IKTFSRPVDIGLLEGGISNDENYEQLKLFRKHCKILISVGQCAITGGVPAYRNTVPLK
                     ECFEEAYLKGPTVVDGGLFPNDPDIPIMLDKVYPCHEVVKIDYFLPGCPPTADTLWAA
                     LTALLTGKEVGLPYELIKYD"
     gene            complement(9097..9810)
                     /locus_tag="PKW60_00230"
     CDS             complement(9097..9810)
                     /locus_tag="PKW60_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPJ97883.1"
                     /translation="MSKHTVNIIIDNVEIQAREGQTILQAADDAGIYIPRLCYLKDLI
                     PGGHCRVCTVKVNGRPASACTFPVSEGLVIENDTEELTTLRRNVVEMLFAEGNHICPS
                     CEASGNCELQAMAYRLGMLTVTQPFLRMPRELDATHPDVFIDRDRCILCGRCARASEA
                     EGKRVFGFEGRGINKRIAVDGMHGLKETELKAADRAAHACPTGCLRVKRVGWKVPVGE
                     RLYDTTPIGSDIEQKQPVG"
     gene            complement(9816..11594)
                     /locus_tag="PKW60_00235"
     CDS             complement(9816..11594)
                     /locus_tag="PKW60_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPJ97884.1"
                     /translation="MSNDYRKVVQEVVAKFGKDRTRLMDIARAVHAKIGYISEDTVGA
                     IAEAVGCHRVEVRDMTSFYAFFSREPKGATMIRLCDAVVERMKGAGAVAKAFEEALGI
                     RFGQTTPDGKISLAYTSCIGMSDQAPSALVNGKVLTNIRPEDVKSIVEAVRAGDLHDG
                     GVVSVKSVESNLRQAGPVIFAPFERGAGIRKALSMSPEEVINEMNKARVRGRGGAGFP
                     LAMKWDFCRKAEGSAHYVVCNGDEGEPGTFKDRVILTECPDLMFEGMTIAGYAVGAKQ
                     GFFYLRGEYEYLLPYLEQVLAKRRRLGLLGENVCGAEGFDFDIRIQMGAGAYICGEES
                     ALIESLEGKRGAPRDRPPFPVQKGYLGQPTSVNNVETLCCAARIMERGAEWFSKIGTK
                     DSTGTKLLSISGDCEAPGVYEVEYGITVEKMLEQVGAVDAQAVQIGGPSGQCIAPKDF
                     GRSICFEDLPTGGSTIIFGKDRDLLECMEGFLEFFVEESCGWCAPCRAGTTFLKMQFE
                     KLLKGRSTREDLQKLEETANTVKFMSRCGLGQTAPNPVLTTLRNFPALYEARIKPGEF
                     IPAFDLREALNEACSITGQKPHDVEV"
     gene            12120..13517
                     /locus_tag="PKW60_00240"
     CDS             12120..13517
                     /locus_tag="PKW60_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPJ97885.1"
                     /translation="MRANMKWLSLLALAAAGCATMGSPGEHAVIRAKNKVIPSLVHIR
                     PVKETFASGQKQEYVVEGSGFIISPDGYVVTNEHVAGDSSLVRCVLYNKDEIDAEVVG
                     TDRYTDIAVLKLKSSETNLPAAELGNSAHIEPGQTVLALGSPHGLSRSISKGIVSVTD
                     RYLGEEGPRPAPYNTWIQTDAAINPGNSGGPLVNLKGEVIGINTRKLSGADNVGFAIP
                     VNTAREVIEAIIDHGYVPRSWIGIRLQEMTSKTDDPTQRGVVVADIDPLGPAAAAGFV
                     PGDVITAVNNVPIHARFEEDLPPVLKRIADLPIGTPASFSVRRGEETQAITVVTMEES
                     DYKGNEVEFSEWGFAAAGLTPAIVQAARLPSSQGIVIAGIEVGTAAAESGLRQGDIVL
                     SVDGKEIAGLTEFQALYDEINAAAKRLVLLDVKRGALTRFELLKLEPGADNDDATPEE
                     LITNEENGEHPDEDE"
     gene            13504..14910
                     /locus_tag="PKW60_00245"
     CDS             13504..14910
                     /locus_tag="PKW60_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPJ97886.1"
                     /translation="MRTNSLAAVWLLGGLLTGVAAAPVAEEAGAFSPAFIASQQKAVA
                     PAICLLNFTAEVESQRTGETQKRSTRALGLIVSPDGLVMTHGHMQSDKSTPSHIRVIV
                     GEGDEDKEYDGVILKKPDDINVCFVRIQGEAGERFPFVTFASEENLVLGERILLFGIM
                     GETLDFARGALTRGISAVIEKPRMTYCIDDAIPFGYVGAPVIDARSRIVGVVGYDLTP
                     AEGGELYVRSGHPLVYQAPLFAKYIQNPPTADALETDHPIAWIGVFSQPLTDDLAEYW
                     GLPKNGGVVISTVVEGSPAEAAGIQRGDVITSFDGTPMRAKKNSEIVGFTKLVRETGV
                     GREIPVKLLRQGQPLDLSIVLTERPVSAKDAEEFVEDTFGLTVRALTTDVRILLNLPD
                     DTEGVIVRRVKSGSWAHLAGMRPGVIILRFGEYPVTTIAEFEDAVNKAREARPAEVPV
                     FCRAGARTGFFRLQPRWD"
     gene            14914..15516
                     /locus_tag="PKW60_00250"
     CDS             14914..15516
                     /locus_tag="PKW60_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97887.1"
                     /translation="MRGLMSIGGALLLAGIAAAGCSGPPKAHYEDLSAQAEFLVLEER
                     WAEAIPVLSESLLLHPEDPGAHFYLGRCWLNVAKFNDRPGPWLVPAEGELRLARQLFL
                     AQGKHSPIARFSDTYFEVICNIERAKVYLRQLTVVAEHPRQLAGATLESILSSLQQAI
                     DDAKAVDPTAPEVIQLENLLKSLSGNESAPEPPASAGTVI"
     gene            15721..16383
                     /locus_tag="PKW60_00255"
     CDS             15721..16383
                     /locus_tag="PKW60_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97888.1"
                     /translation="MVSVVGREEAVSEELERLAQQLRDTQTGWVARRDAAQALGKAVS
                     FALDALREYSDEMDVDVRRAVDQALGEASAALAGVPARPPVRQYTLEELAIACAKDGE
                     RVVAPHGKGYVVQVTLKSGRHQAVFLNHHVRKDGAELIRVFTVCGKPRADAFEWALRA
                     NMKISLGALALTGEGDEERMVLTNRFLARAATPSQIKASVKELAVYGDWIESKLGELD
                     DF"
     gene            16383..16913
                     /locus_tag="PKW60_00260"
     CDS             16383..16913
                     /locus_tag="PKW60_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPJ97889.1"
                     /translation="MTNMLPVNSPAALIAALRQGRGVGARLPRVQLEGADLSGVDLTD
                     ARLPGANLRRANLSKAILRGAFLDGADLSDADCSGADFEFAVLGGATLTRAVFRDAVL
                     RRANLVGASGVEACFDGADLYYARPGNADFTGAVFHNAQIQRAIFRRANLTNADFTGA
                     EGQANFENATQTGMRR"
     gene            16913..17470
                     /locus_tag="PKW60_00265"
     CDS             16913..17470
                     /locus_tag="PKW60_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPJ97890.1"
                     /translation="MFKHFLTSVNESNAFILACDETRDAILIDAGAFEPAITAFVEAK
                     HLKLKSVFITHDHYDHTGGLREIVDRYDAQVYAGSANPGGCRADTVGHGDAIRVGGLE
                     GRVLATPGHTPDALSLTFPGMVFTGDALFAGSVGGTASPALARQQSEAIKAHIFSLPA
                     DTEIHTGHGPSSTVAIESRYNPFFV"
     gene            17654..18427
                     /locus_tag="PKW60_00270"
     CDS             17654..18427
                     /locus_tag="PKW60_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPJ97891.1"
                     /translation="MICAVCLAATATTALADDEAAPKEKWISLFNGKNLDGWIPKIAK
                     HECGDNYANTFRVEDGILKVCYDGYGPFDDQFGHLFYKEPFSNYRLRVEYRFVGEQIE
                     GGAGWAYRNSGIMIHGQTPQSMGKDQSFPVSIEVQLLGGSGSGERTTGNLCTPGTHVV
                     IDGKLEKKHCINSSSKTFHGDQWVTAEVEVRGNTIKHFINGELVLTYCDPQLDDGDKD
                     AQKLLEAGQAPLLTSGTISLQSESHPVEFRKVEILKFDE"
     gene            complement(18518..19465)
                     /locus_tag="PKW60_00275"
     CDS             complement(18518..19465)
                     /locus_tag="PKW60_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HPJ97892.1"
                     /translation="MHKRIRTVGFTLIELLVVIAIIGILAAILLPALARARESARRAS
                     CANNLKQIGLMLKMYANEAKGEKLPSMLRYTSLSDSDDPASYVSQCGLPNPPVSPALG
                     GDAEFIFDGPSVIPEYLTDPNVMVCPSDSSAAGLVEAGRFNVNGDPDGNLDPCAISAI
                     SYMYVAWALQGKEDYMNAGVEENDAFGWTPFGTPDTVSTNFILAIFNVLTESANGNPA
                     AFEQDVTYVNDYGTRTLYRLREGVERFFVTDINNPAGSALAQSQLPIMWDLVSTDVTE
                     FNHVPGGSNVLYLDGHVEFVRFPGEFPVTRAFAGVISAF"
     gene            19850..>21273
                     /locus_tag="PKW60_00280"
     CDS             19850..>21273
                     /locus_tag="PKW60_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97893.1"
                     /translation="MPARLQQGALILLLLILPAASSAASRETRGQSNAARPPLRVVVT
                     PDELVNEPASPLLYGHLIQSGFGRQVDGLWAEMLFNRSFEAVTPFKESVWGWLHRRPD
                     DDLSTEDWWHSGYEETDWYIAPGNPEAHLGYSAYWDFRHGERSAHVTNRNNTSAAILA
                     QDGLWLRKGMSYRLRGCLRHGDLMDPDTPPINITVGLYGEARTDSPLATATLRGIGST
                     WQQVECELPNSDYDGPATLGITVPPGITLALDGLSLMPSDNVQGWRRNAVEALKRVRP
                     RILRWPGGSSASFYFWRDGIGPAHERRPRESTYWGGLENNDAGTAEFVALCREIGAEP
                     LICVNVLTGSPAEAADWAAYCNAPPEHPQGALRALHGHAEPFGVTYWEIGNEPYRKYD
                     ALEYARRCVEFARTMKAADPGIQLIMAVYWRFHAFLPQMLEIAGPHIDFVADRELDES
                     HLRGVLETLREYNARTGRSIRLCNT"
BASE COUNT         4240 a         6553 c         6439 g         4041 t
ORIGIN      
        1 tgatccgcgt gatcaacttc ctgacgtcgt ggccgatcct ggtcgtgttg gcgggcatcg
       61 tggccctgat tgttgtgtac tgggtatggc agcgcaatcc cgtcaaccgc ctgctggcgg
      121 atcggttgaa gctgcgcgtg ccgctgctgg gccggatcag ccagaaatac gccgtggctc
      181 agatgaccag ctcgttgtcg atgatgctgc ggagcggcct ttccatgatg gtcacgctgg
      241 atctggcgcg cagcgccatt cacaaccagg cggtggccca ggtgctccag aaagtgcgca
      301 gttccgtgga acagggcagc ggcattgaag agcccctgcg cgctgagccg aaagtcatcc
      361 cgcccgtggt cacggatatg ctggtcaccg gcgaagaggc gggtcaattg gatcgtgtcg
      421 ccgcgcatat cgcggacacc tacgaagaag aagtccagat tgacatcggc accctgacgg
      481 atctgctccc ggtgatcctg gtgatcatcc tcgggatcgc ggtgctgatg atcgtgctgg
      541 ccttcttcct gccgctcatt tccagcatcg accagttgac ctccgccaca tcctgaggcg
      601 gcgtccacaa atctgtatcg gcggggcctg acaacttgtc acggccccgc ctctgcgttt
      661 atgcttgtag ggattttcgg acagagactc caaaacagac ccccggagca gaacactacc
      721 tgccggggag gagaggcatt ttcagtgagc atagcgacat tactggaaga acgggcgcaa
      781 caacacgggt cgcgcacatt tctgatttgc gaagacaggg aatattcgtt cgagacgatg
      841 caggaacaca gcgttcgcgt ggcggtgaac ctggccgggc gcggcgttca gcgggacgac
      901 aaaatcgtgc tgctgatggg caattgcctt gaatatctct atgtttttct cggagcagga
      961 cgcattggcg cggtggttgt tccggtaaat cccacactga aagccgagga aatcacgcac
     1021 atcgcgaata actcagaggc cgaaacgctg attaccgcgc cggaactggc gcccatgatc
     1081 cctcagctcc gcgccgccct gccacggatc aagcgatatt ttgttctggg cgagagcacc
     1141 gaagacgccg agtcgttctc cgagctgctc gccccggttg cctccgttcc gcccatcctg
     1201 gctggtccgg acgacaccgc ggcgctcatt tacacctccg gcaccaccgg cgcgcccaaa
     1261 ggcgtgatcc tgacccaccg aaactacttg tccaacgctc tcatgcttgt caaatcgcag
     1321 accgtggtgg aggacgaccg gtttttctgc gtcttgccgc tgtttcatgt caatgcgcag
     1381 gtggtaacca ttctgacgcc gctgatgggc ggggcttcgg tcctgttgat gaagaaattc
     1441 aaccccttcg cgatcctccc gatgatacag aagtatcgcc cgacgatcat gagcgccgtt
     1501 cccaccatct acaacgtgat gtgccggctg gccaaggctc aggaacacga atacgatctg
     1561 tcgtccattc gctcgtttgc caccggcgcc gcgcccatgc ccgaagagac ctatcacgag
     1621 gcgctccgcg ccttgaaacg gcccatcatt ggcggctacg ggctgacgga ggccacctgt
     1681 gccagcgccg ccgcggacgc gcgcgatccc atcaaatgga actccgtggg cagcccgctc
     1741 ccctacacca tcatccggat cgtcggcgaa gacggcaagg acgtcccgtt cggcgaaatc
     1801 ggcgaaatac tcattgccgg ccccaccgtg atgaagggct actacaagaa tcccgacgcc
     1861 accgccgaag tgctgcgcga cggatggctg cacacgggcg atctcggaca gtttgaccgg
     1921 gacggatacc tgtatatcgt gggccgggtg aaagacatga tcatccgggg cggccagaac
     1981 atctacccgc aacagatcga agacgtcatt tcgcgcctgc cgggcgtgga agaatgctgt
     2041 gtggtcgggg tggaagaggc gcgctggggc caggaagtgc tcgccgtcgt caaacgcacg
     2101 gagaacatca acctggcgga gagcagcgtc atccagcatc gccgtcaata tctggcgagc
     2161 tacaaatgcc ctgcgtttgt gcgtttcgtc gacgaactgc cgaaaacccc gaccggcaaa
     2221 atcaaaaaag tggaggtggc cgcgagcttc gcgaacatcg caaaaaacgc gtaagcccgc
     2281 gcctaaaccc gaacgaggag aaaatttggc catgatgcac aaacggacac tcggcattct
     2341 cttgtgcgcg gtggttctcg ccgccggcct gaacggatgc gccacgggta aagctcacgt
     2401 ctccagcgaa gccgccatac gagagactct ggccgcgtgg aaagccggac tcgaaagcca
     2461 taacctggac aggatcatgt ccgtggtctc cgaggagttc gtcagtggcg acggaggcag
     2521 caaagccgac ttccgcagct acgtcgccag gctcatcgat gacggcacgc tgtctggcgc
     2581 gaagatgaac attgagactg cccgcgtcac tatcgaaaaa gacatggcca cagttgaaaa
     2641 tgttgtcctg agcggcgatc gcggcagtgc ccagcttgag atggatctta agaaagagag
     2701 cggcggcgcc tggcgcatcg taggtctgga ggcctactga gcacagctcc ccggcggccg
     2761 gaacgcccac ccggttgcca ggcgctgaaa tggcgagaag accgccggga aaagaggaac
     2821 gccatgaaag tgcaggattt cgcgtaccag gtcgccattc gcactatcga gctcttggaa
     2881 gagacccagc actacaagat ccctgagaat ctccgcaagg aggtcggcca gaaaattctc
     2941 ggcgaggtcg ataagcttct tcagaagtcg aactgaaagc gggccaccgc cagacatccg
     3001 cgagagtccg gctggagccc gtttctgaga gcgtgaaagg aaagcgctct ccaaaaatgc
     3061 cgttttttga tggcattgac ctgccgctat tcgttatgat cacgttttgt tcccgggttc
     3121 gtgcgggtgc gcgcacaacg ggacaatgcc ctgagcaatt ctccaggagc cgatgcctgt
     3181 tctatgcttg aaaaagccaa atccccgggg aaatcccgta aaactgctcc gaaacgcaag
     3241 ccggttcttt cgaaaaaccg caaggcaagc gcggacggcc ttcccgccgc ccacgccacg
     3301 tcgcgcgaca gcatcgtcat caaaggcgcc cgcgagcaca acctcaaaaa cctgagcctg
     3361 gcgatccccc gcgacaagct ggtcgtgatc acgggcctga gcggatcggg caagtcgagc
     3421 ctcgcgttcg acacaatcta tgcggaaggc cagcgccgct atgtcgagag cctgtcgtcg
     3481 tacgccaggc agtttcttga acagatggaa aaacccgaca tcgactcgat cgaaggcctg
     3541 agtccggcca tctcaatcga gcagaagacg acgcaccgca acccccgatc gaccgtgggc
     3601 acggtcaccg agatctacga ttacctgcgc ctgctcttcg cgcggatcgg cacgccgcac
     3661 tgcccggatt gcggaaaacc catccagtcg cagacgcctc agaccatcgt cgacggcatc
     3721 ctggccttgc ctgaaggcac gcgcgttcaa ttgatggcgc cggtggttcg tcagcgcaag
     3781 ggcacgtacc agaagacctt cgaaaacatc aaacggcggg gctatctgcg ggtccgggtc
     3841 gacggcgaat tccgcacggt agatgaagag atcgaactgg aacggtatgt caagcacgac
     3901 atcgacgtgg tcgtcgaccg cctgctggtc aaaccgggca tcggacggcg cctgacggat
     3961 tcggtcgagg cctgtctgga actgggcgaa ggcatcatcc ggatctggcg cgagactccc
     4021 gacaaaaccg tgagcgaagt gttgcagagc gagcatttcg cctgcgtgga ttgcgggctg
     4081 agcttcgaag agctctcccc gcgcatgttt tcgttcaaca acccgcacgg cgcgtgcccg
     4141 ctatgtacgg ggctcggcga ggtgatcgag gtcgagcccg atctggtggt tcccgaccca
     4201 tcgctgtcca tcgcgaaagg agcggtacgg gcgtggagca tgcggcgcgt gctggacggc
     4261 atgtaccgcc acgccttgcg ctgtgtatgc cggcattacg gccaggatcc cgccaccccg
     4321 tgggaagaac tccccgaaga cttccggcag atcgtcctgt acggctcggg ggacgaggtc
     4381 atcgatttca cctatacagg gcgccgtaac acattcgaac tccagcgccc cttcgagggc
     4441 gtgattccga atctcacgcg gcgctaccac gacaccgaat ccaaccgtgc ccgcgagatg
     4501 atcagcgagt tcatggcttc gcggccctgc ccggactgcg gcggtgcgcg gctccggccc
     4561 gaatcgcgct cggtaacggt cggtggcaag accatcatgg acgtggtggc gttgtcggtc
     4621 cgccacgcgc tgaccttctt cgaaaagctg agccttaaca agacgcagcg tctgatcgcc
     4681 gagcgggtga tgaaggaaat ccgcgagcgc ctgtcctttc tcgtaagcgt ggggctggat
     4741 tacctgtcgc tcgatcgcaa cgccggcagc ctgtcgggcg gggagtcgca gcgcatccgc
     4801 ctcgccacgc agattggctc ggggctgatg ggcgtgctgt acatcctcga cgagcccagc
     4861 atcggcctgc accagcgcga caacaaacgg ctcatcgcta cgctggaacg catgcgcgac
     4921 ctcggaaaca cggtgatcgt cgtcgaacac gacgaggaga caatccgccg ggccgaccat
     4981 gtgatcgatt tgggtccggg cgcgggggtc cacggcgggc aggtggtcgc gcagggaacg
     5041 ccccgggaag tcgcggccaa ccccgactcc ctcacggggc agtacctgtc gggccggttc
     5101 cggatccgcg tgccggaaac gcgccgtaaa tccaacggca aggccctcaa ggtgttcggt
     5161 gcggcacaca acaacctcaa ggcgatcgac gtggagatcc ctctgggcgt gctcacctgc
     5221 gtgacgggcg tctccggttc gggcaaatcc agcctcatca acgaaaccct gtacccggcg
     5281 atcgcgcgcg ggttatacgg ctccctgcgc gcgcggcccg gccggcatga gcgtatcgaa
     5341 ggcctcgagc acctggacaa ggtcatcgac atcgaccagt cgccaatcgg ccggacgccg
     5401 cgctcgaacc cggcaacgta tacgggcctg ttcacgccga ttcgtgagct gttcagcaaa
     5461 gtgcccgaag cccgcgcccg gggatacaaa cccggccggt tcagcttcaa cgtcaagggc
     5521 ggccgctgcg aagcctgcca gggcgatggc gttcttcaaa tcgaaatgca tttcctgccg
     5581 gatgtctacg tgccgtgcga tgaatgccgg ggtaaacgat acaatcgaga caccctcgag
     5641 atcctctacc gcggacgcag cattgccgat gtgctcaaca tgacggtcga agagggctgc
     5701 gacttcttcc ggaacgtcca ggcgatctac agcaaactca gcacgctgtt tgatgtcgga
     5761 ctgggctaca tcaagctggg gcagcccgcc acgaccctgt cgggcggtga agcccagcgc
     5821 gtcaaactgg cgacagaact gtcgcgccgc cagaccggca agaccttcta tattctcgac
     5881 gaacccacca cggggctgca tgccgtagac gtggacaagc tgctgcaggt gctgcaccgc
     5941 ctggtcgagc acggcaatac ggtggtcgtt atcgagcaca accttgatgt catcaagacc
     6001 gcggactgga tcatcgatct cggccccgaa ggcggtgatc agggcggcaa ggtggtcgcg
     6061 gccggagaac ccgaaacgat cgcagcctgc gccgcgtcat ataccggcct ccacctgcaa
     6121 cccctgttcc gcgccaaagc ccgctgaatg caaaacgcgc cgggccccgg aagaactccg
     6181 attcccggcg cgcggccgtc agtatctcgt tttcagcgag ccgttcagac ctttagtttg
     6241 gctttcagcg tggccacgag ggttttggga tcgatgggtt tctgaaaaat gcccgccagc
     6301 cccagctccc gcgcgtccag attgtagcgg acggtgtcgc ccgcggagct cagcatataa
     6361 acagggcccg cgtagccgat ctccttgagt ttctgcgcca gtgtgctgcc ggaatccacg
     6421 ttctccatca tgagatcgac gatgatggca tccggctgca cacgctcgac ggctttcagc
     6481 ccttcttcgc cgttcgacgc ctcgcccacc gaaaaccctt cggcctcgag gacgatgcgc
     6541 aacgtcgcgc gaatatccgg gtcgtcatca atagtgagaa ccgtcttctt gctgtccatg
     6601 ctagtctcct tatccggccg tatcagggcc tggatgcatg ccaacgcact ggtcatattg
     6661 tcgcgggccc tgggagtgag gccctcttca aactcgaacg catacccgcg cacggccagc
     6721 acatacgcct caggcgccgc gtggaaatga tccacgcaca aggccagcac ggactccggt
     6781 gaaatgctgt ggctcgtgaa ggtgatctcc gccgcaggag cgatccgcgt gagcgcatac
     6841 ggctccggcg cgtccacact ggcatccacg aacacgacca gctcgtgttc agccagcgcg
     6901 gcggcgtctt cgatattcaa ctgatacggg tcgtccgttg taacgttcga cagcgcccat
     6961 gacgccacgg tgttggcaat cgccgggccc agcccgtcgt cctgccgccc cgggttccca
     7021 tacccgataa tgagcgcgcg ccgatcgttc acccgcggaa tttctcgtcg atcaacgtgc
     7081 ctgccgcatc caccagttcg acgtgcagcc gcatcttacc cagggcatgg gtggcgcagc
     7141 tcaggcacgg gtcgtaggcc cggatgccca cctcgatatg gttgagcagg ccttccgtga
     7201 tctcactttc gcccttgcct ttgaggtgct gatcggcaat gacctgcacc gtgcggttca
     7261 tcggctcgtt gttgctggtg gtcgagacga tcagattggc cagggtgatc tgatccgtct
     7321 cggtgtcgat ttcataatga tggaagagcg tgccgcgcgg ggcttcaatc caggcgtagc
     7381 ccttttcgcg tttctttcct tccgccacga gctccgtgcc ctgcaggtcc gggtcgtcga
     7441 gcagttcgcg gatcttctcg gccgaatgca ggatctcgat gcaccgcgcc cagtggtacg
     7501 ccatgctcgc atgcaccggt ttgccgttgc cgagcgccat aaactcctgg cgggctttct
     7561 cggcgagctc ggtatcgatg aaatcgcagc aattaacacg ggagagcggc ccgacgcggt
     7621 accagccctt ttccttgccc agcgagcgga tgtgcgggaa cttcatgtag gtccagggcc
     7681 gcacttcctc gccgatgtag tccgcgtatt cggccggctg gactttgtca aagatcttgt
     7741 tgccgtcggc atcgatggcg cgcagtgtcc cgtcgtacag atccatgcag ccatcgtccc
     7801 gcacgataga cacatggttc gactcgaagc tgccgaaatt gagaaggaat tccaggttct
     7861 ctttcgtgag cttcttgcag agctcaaccg cttctatcgc ccactcgacc atggtgtcgg
     7921 cgtctttttt gagggcgtcg cgctcttcga tcgtcaggtt cttgttgatg ccgccgggga
     7981 tagcgcccgt gggatgcacc tttttgccgc cggtcgcctt gatgacttcc tggccgaact
     8041 tgcgcatcag caccgctttt ttggcgactt cgggataggc ttcgatgacg ccgatcacgt
     8101 tgcgtttctc gaccggcgca tcaaacccga acagcaaatc cggagacgcc agatggaaga
     8161 aatgcagggc gtgcgactgg tagacctgcc cgtaatgcat caggcggcgc atcttttcgg
     8221 ccgtgacggg aatcttgtcc acacccacca gcatgtcggt cgctttggcg ccggccaaat
     8281 ggtggctcac cgggcagatg ccgcacaagc gctgcaccat gaccggggct tcccaataga
     8341 agcggccctt ggcaaaacgc tcgaagcccc gaaattcgat aatgtggaac cgggcctgtt
     8401 cgacgttgtt gttctcgtcc atcacgatgg tgaccttgcc gtgtccttcg atacgggtca
     8461 ccgggttgat gacgattttt cgctttccgt tctctgcggt ctttgccata gtatgtgcct
     8521 ccacggcgat cagtcgtatt tgatcagttc gtacggcagc ccgacctctt tcccggtgag
     8581 caacgccgtc agcgccgccc agagggtatc cgcggtaggc gggcacccgg gaagaaaata
     8641 gtctatcttg acgacctcgt ggcaaggata gaccttgtcc agcatgatcg ggatatcagg
     8701 gtcgttggga aacaggcctc cgtctacaac cgtcgggccc ttcaggtacg cttcttcgaa
     8761 gcattccttc aggggcacgg tgttccggta agccggcacg ccgccggtaa tggcgcattg
     8821 ccccaccgag atgaggatct tgcagtgttt acgaaacaac ttcaattgct cgtaattctc
     8881 gtcattgctg atcccccctt ccaggaggcc gatatcgacc ggacgcgaaa aggtcttgat
     8941 atcgttgatg ggcgacttgt ggaactccac cagctcaacc aggtcgagta tccgctcatc
     9001 aatgtccagc aaagacatat gacagccgaa acagcccgta aagggggcgg tcgctactag
     9061 gggcttcgcc atggtgtcta ccctttcctg aatcgttcag cctaccggct gtttttgctc
     9121 gatgtcgctc ccgatgggag tcgtgtcata cagccgctcg cccaccggca ctttccatcc
     9181 cacgcgcttg acccggaggc atcccgtcgg acaggcatgg gcggcccggt cggcggcctt
     9241 cagctcggtt tccttgagac cgtgcatccc atcgaccgcg atgcgtttgt tgatgccgcg
     9301 gccttcaaaa ccaaacacgc gtttgccttc cgcctcagag gcccgggcac agcggccgca
     9361 gagaatgcag cgatcccggt cgataaagac gtccggatgg gttgcgtcga gctcgcgcgg
     9421 catgcggaga aatggctggg tgaccgtgag catgccgagg cggtaggcca tcgcctgcag
     9481 ttcgcagtta ccgctggctt cgcacgacgg gcagatgtga ttgccctcgg caaagagcat
     9541 ctccaccacg ttgcggcgca aggtggtcag ctcctccgtg tcgttttcaa tgacgagccc
     9601 ctcgcttacg ggaaacgtgc aggcgctggc aggccggcca ttgaccttca cggtacagac
     9661 gcggcagtgg ccgccgggaa tgagatcttt caggtagcag aggcgcggaa tgtaaatccc
     9721 ggcatcgtcg gcggcctgca ggatggtctg tccttcccgg gcctggattt cgacgttgtc
     9781 aatgatgatg ttcaccgtat gtttgctcat ccgaattaca cctccacgtc gtgcggtttt
     9841 tgcccggtaa tggagcacgc ttcgttgaga gcctccctga gatcgaatgc cgggatgaac
     9901 tcgcccggct taatccgagc ctcatacagg gccgggaaat tgcgcaacgt ggtcagcacg
     9961 ggattgggtg cagtctggcc aagtccgcaa cgactcatga atttcaccgt gttggctgtc
    10021 tcctcgagtt tctggaggtc ttcccgggtg cttcggcctt tcagcagctt ttcgaactgc
    10081 atcttgagga aggtcgtgcc cgcgcggcag ggcgcgcacc atccgcacga ttcctcgacg
    10141 aagaactcca ggaatccttc catgcactcg agcaagtcgc gatcttttcc aaagatgatg
    10201 gtcgaaccgc cggtgggcag atcttcgaag cagatgctcc gcccgaaatc cttcggcgcg
    10261 atgcactggc ccgaaggccc gccgatctga acggcctgcg cgtccaccgc gccaacctgc
    10321 tcgagcattt tctcgacggt aatcccgtac tcaacttcgt aaacgcccgg cgcctcgcag
    10381 tcgcccgaga tgctgagcag cttggtcccg gtcgagtcct tggtgccaat cttggaaaac
    10441 cattccgcac cgcgttccat gatgcgcgcg gcgcagcaga gcgtctcgac attgttcacg
    10501 ctggtaggct ggccgagata tcctttctgt accgggaacg gaggtctgtc gcgcggagcg
    10561 ccgcgttttc cttccagcga ctcgatcagc gccgactctt cgccgcagat gtacgcgccg
    10621 gcgcccatct gaatgcggat gtcaaaatca aatccttcag cgccgcacac gttctcgccc
    10681 agcaggccca ggcggcggcg cttggccagc acctgctcca gatagggcag caggtattca
    10741 tactcgccgc gaagatagaa aaatccctgt tttgcgccga cggcgtatcc ggcgatcgtc
    10801 atcccctcga acatcagatc ggggcattcg gtcaggatca cgcgatcctt gaacgtgccg
    10861 ggctcgcctt cgtcgccgtt gcagacgacg taatgcgccg agccttccgc cttgcggcag
    10921 aaatcccatt tcatcgccag ggggaatccc gcgccgccac gcccgcggac acgggctttg
    10981 ttcatttcat tgatgacttc ttcggggctc atgctcaggg ctttccggat gcccgcgccc
    11041 cgctcaaacg gcgcaaaaat gaccgggccc gcctggcgca ggttcgattc aaccgatttc
    11101 acgctgacga cgccaccgtc atggaggtcg ccggcacgga ctgcctcaac gatcgacttg
    11161 acgtcttcag gccggatatt ggtgagcacc ttgccgttta ccagcgccga aggggcctgg
    11221 tccgacatcc cgatgcacga ggtgtacgcc aggctgatct tcccgtcggg agtggtttgg
    11281 ccaaagcgga tgcccagcgc ttcttcaaac gctttcgcaa cggcgccggc gcccttcatg
    11341 cgctcgacca ccgcgtcgca caggcgaatc atcgtcgcgc ctttgggctc acgcgagaag
    11401 aatgcataga acgacgtcat gtcgcgcact tccacccggt ggcagcccac ggcctccgca
    11461 atcgcgccta cggtgtcttc cgagatgtag ccgatctttg cgtgcacggc gcgcgcgatg
    11521 tccatcagac gcgtcctgtc tttgccgaac ttcgctacca cctcctgcac gaccttcctg
    11581 taatcattgc tcatgcttac gggcctccag cgttgcgaca gcttcgctta cggaaccatc
    11641 ggataatcag tgacttatac ggcaaccgcc aagacgagga aatgcgggta agactgaatc
    11701 caccgtctaa tcgtgaactt tatcacaatt gggagcgctt ttcaaccgta taagccgcat
    11761 tcccgcgatt ctttcagaga gaatgccgcc atgtcaattg agtagcttgc gccacatcag
    11821 agacgatgct ggggatgatg gcgtcgcgcc ctttgcgatg gtgccggcat ggaaacaggg
    11881 cgatgccgtg caagcgactt ctggacggat tgggcattgc cgccgcggag cagcaacccc
    11941 acggcatccc gcttgaacgc ttcgtcataa ttccgtttgg tcacgtcagg tcacatggca
    12001 cgacgatatc atcgccgaga aaagtatggc tggcgcgagt cgaatacctg tagaatggcc
    12061 gccggttaca ttataattgc cggaacaata tcgggaatcc ttggacaagg agagccgctt
    12121 tgagagcaaa catgaagtgg ttgtcgctgc tcgcgctggc agccgcggga tgtgccacga
    12181 tgggcagccc cggcgaacac gccgtgatcc gggcgaaaaa caaggtcatt ccgtcgctgg
    12241 tccatatccg ccccgtgaaa gaaaccttcg ccagcggtca gaagcaagaa tatgtggtgg
    12301 agggcagcgg cttcatcatc tcgccggacg gctacgtggt taccaacgaa cacgttgcgg
    12361 gcgacagcag cctggtacgc tgcgtgctct ataacaaaga tgaaatcgac gcggaagtgg
    12421 tgggcacgga ccggtacacg gacatcgccg tgctgaaact gaagagcagc gagacaaatc
    12481 tgccggccgc cgaactgggg aattcggctc atatcgagcc tgggcagacc gttctggcgc
    12541 tgggcagtcc ccacggcctg tcgcggtcca tatcgaaggg gatcgtgagc gtcacggacc
    12601 gctatctcgg cgaagaagga ccgcggcccg ccccgtataa cacgtggatt cagacggacg
    12661 cggccatcaa ccccggcaac agcggcggac cgctggtcaa cctcaaaggc gaggtcatcg
    12721 gcatcaacac ccggaaactc tcgggcgccg ataacgtcgg gttcgctatc cccgtgaata
    12781 ccgcccgcga agtgatcgag gccattatcg accatggtta cgtgccgcgg agctggatcg
    12841 ggattcgcct gcaggaaatg accagcaaaa cggacgaccc cacccagcgc ggcgtggtgg
    12901 ttgcagacat tgacccgctg ggacccgccg ccgcggctgg tttcgttcct ggcgacgtga
    12961 tcacggcggt aaacaatgtg ccgattcacg cccgctttga ggaggacctg cctccggtcc
    13021 tcaaacgcat cgccgacctg cccatcggca ccccggccag cttttccgtg cgccgcggcg
    13081 aggaaacaca ggccattacc gtggtgacca tggaggaaag cgactacaag ggaaacgaag
    13141 tcgaattctc ggaatggggg tttgccgcgg ccggcctgac gcccgccatt gtgcaggcag
    13201 cgcgcctgcc gtccagccag ggtatcgtca tcgcagggat tgaagtgggc accgcagccg
    13261 ctgaatccgg tctccggcaa ggcgatatcg tgctcagcgt ggacgggaag gaaatcgccg
    13321 gcttgaccga gtttcaggcg ctttacgacg agatcaatgc cgcggcgaag cgtctcgtgc
    13381 tgttggacgt caaacggggc gcgctgaccc ggtttgaact gctgaagctc gaacccggcg
    13441 ccgataacga tgacgcaacg ccggaagaat tgataaccaa cgaggaaaac ggagaacacc
    13501 ccgatgagga cgaatagcct cgctgccgtc tggctcttgg gcgggctgct cacgggcgtc
    13561 gcagccgccc ccgttgcgga agaagccggc gccttttccc ccgcgtttat cgcttcgcag
    13621 cagaaagcgg tcgctccggc gatctgcctg ctcaatttca ccgccgaggt ggaatcgcag
    13681 cgaaccggcg aaacccagaa acggtcgacg cgcgcgctcg ggctcatcgt gtcgccggac
    13741 gggctggtga tgacgcatgg gcacatgcaa tccgataaat ccacgccgtc ccacatccgc
    13801 gtgattgtcg gggaaggtga cgaagataag gaatatgacg gcgttatctt gaaaaaaccg
    13861 gacgatatca acgtctgttt tgtgcgaatc cagggcgagg ccggcgaacg gtttcctttt
    13921 gtgaccttcg cttcagaaga gaatctggtt ctgggcgagc ggatactact gttcgggatc
    13981 atgggcgaga cgctcgattt cgcgcgcgga gcgctcacgc gcgggatcag cgcggtgatt
    14041 gaaaagcctc gcatgacgta ctgtattgac gacgccattc catttggata cgtgggtgcg
    14101 ccggtgatcg acgcacggag ccgcatcgtg ggcgtggtgg gctatgatct caccccggcg
    14161 gaaggcggag agctttacgt tcgcagcggc catccgctcg tgtatcaggc gccgctgttt
    14221 gcgaaataca tacagaatcc cccgaccgcg gacgcgctgg agaccgacca ccccatcgcc
    14281 tggatcggcg tgttcagcca gccgctgacg gacgatctgg cggaatattg gggtctgccc
    14341 aaaaacggcg gggtggtgat ctccacggtg gtggagggtt ctccggccga ggcggccggc
    14401 atccagcgcg gcgacgtcat taccagtttc gacggcacgc cgatgcgcgc caagaaaaac
    14461 agcgagattg tcgggtttac caaactggtg cgcgaaaccg gcgtgggccg cgagatcccg
    14521 gtcaaactgc tgcgccaggg ccagcccctg gatctctcca ttgtgctcac cgaacggccc
    14581 gtttcagcca aggacgcgga ggagtttgtc gaagacacct tcggcttgac cgttcgtgcg
    14641 ctgacgaccg atgtgcggat actgcttaac ctgcccgacg ataccgaggg cgtgatcgtg
    14701 cgccgggtca aatcggggag ctgggcgcat ctggcaggca tgcggccggg agtgatcatc
    14761 ctccgtttcg gcgaataccc ggtgaccacc atcgcggaat tcgaagacgc cgtaaacaaa
    14821 gccagggaag cgcggcccgc cgaggtgccg gtgttctgcc gggcaggggc ccgaaccgga
    14881 ttcttccggc ttcagccccg ctgggactag ctcatgcgag gactgatgag catcggcggg
    14941 gcactgctgc tcgcgggaat tgcagcggca ggctgctccg gaccgccaaa ggcgcactac
    15001 gaggatttga gcgcccaggc cgagttcctc gtgctggagg agcgatgggc agaagccatt
    15061 cccgtgctct cggaaagcct cctcctgcat ccggaggatc ccggtgcgca tttctacctc
    15121 ggacgctgct ggctcaacgt ggcgaaattc aacgaccggc ctggtccatg gctggtgccc
    15181 gcggaagggg aactgcgcct ggcccgccaa ctctttctgg cccagggcaa acacagcccg
    15241 atcgcccgct tttcggacac ctatttcgag gttatctgta atatcgagcg cgccaaggta
    15301 tacctgcgcc agttaaccgt ggttgccgag catccgcgcc agctcgccgg ggcgacgctc
    15361 gagagtattc tctccagtct gcagcaggcc atcgacgatg ccaaggccgt cgatcccacc
    15421 gcgccggaag taatccaact tgaaaacctc ctcaagtcat tatctggcaa tgaatcggct
    15481 cccgaacccc cggcaagtgc cggaacagtc atctgaagag catcagccct gcgcagtgag
    15541 agcgcgttga caccggctcg cctgctttgg cctctacctt cgcatggatc tgccaccttc
    15601 cacagtcaaa accgacgtgc cgggccgcgc cggagacagg tccgagcccg cagctcggcg
    15661 gcggctgagg cagttcgagt cccggcaggc cagtagcgcg ccatggaaag gtctgataga
    15721 atggtatccg tagtggggag ggaagaggct gtgtcagaag aactcgaaag actcgcccag
    15781 cagctgcggg atactcagac gggctgggtc gcgcggcgcg acgccgcgca ggcgctcggc
    15841 aaggccgtga gcttcgcact ggacgccctg cgcgaatatt cggatgaaat ggacgtggac
    15901 gtgcggcgcg cagtggacca ggcgctcggc gaggcgagcg cggcgctggc cggcgtaccg
    15961 gcccgtccgc ccgtcaggca atacaccctg gaagaattgg cgatagcctg tgccaaagac
    16021 ggcgagcgcg tggtcgcccc ccacggcaaa ggttacgtgg tgcaggtgac cctgaaatcc
    16081 ggacggcacc aggcggtgtt tctgaaccat cacgtgcgca aggatggcgc ggaactgatc
    16141 cgcgtgttca cggtctgcgg gaaaccgcgc gcggatgcct tcgaatgggc gctgcgggcg
    16201 aacatgaaga tttcgctggg cgcattggcg ctcaccggtg aaggcgatga ggagcgcatg
    16261 gtgctgacga accgcttcct tgcccgcgca gcaacgccat cgcagatcaa ggcgtccgtg
    16321 aaagaacttg cggtttatgg cgactggatt gagagcaaac tcggcgaact ggacgatttt
    16381 tgatgacaaa catgctgcct gtgaactcgc ccgcggcctt gattgcggcc ctgcggcagg
    16441 gacgaggcgt gggcgcccgg ttgccgcggg tccagcttga aggagcggac ctttccggcg
    16501 tggatttgac ggacgcccgc ctgccgggcg cgaatctccg ccgcgccaac ctctccaaag
    16561 ccattctgcg gggggccttt ctcgacggag ccgatctctc ggatgccgat tgcagcggcg
    16621 ctgacttcga attcgcggtg ctgggcggcg caacgctgac gcgcgccgtg ttcagggatg
    16681 cggtgctgcg gcgcgcaaac ctggtgggcg catccggcgt ggaggcctgt ttcgacggtg
    16741 cggatcttta ctatgcgcga ccgggaaatg ccgacttcac gggcgcagtg ttccataacg
    16801 cccagataca gcgggccatc ttccgccggg ccaatctgac caatgccgat ttcacggggg
    16861 cagaaggcca ggccaatttc gaaaatgcca cccagacagg gatgcgccgg taatgttcaa
    16921 gcattttctg acatccgtga atgaaagcaa cgcgttcatc ctggcctgcg acgaaacccg
    16981 ggacgccatt ttgatcgatg ccggcgcgtt tgagccggcc atcacggcgt ttgtcgaagc
    17041 gaagcacctg aagctcaaaa gcgtgttcat cacccatgac cattatgatc acacgggcgg
    17101 actccgtgaa atcgtggatc gctatgatgc gcaggtctac gccggctcgg cgaatcccgg
    17161 aggttgcagg gccgataccg tagggcatgg cgacgcaatc cgcgttggcg gcctggaagg
    17221 tcgcgtgctc gccacgccgg ggcatacgcc cgatgcgttg agcctgactt ttcccgggat
    17281 ggtctttacc ggcgatgcgt tgtttgccgg ctcggtgggc ggcaccgcat ctcccgcgct
    17341 cgcccgtcaa cagtccgagg ccatcaaggc ccatatcttc agcttgcccg cggacaccga
    17401 gatccatacc ggccacggcc cgtcgtcgac cgtggccatc gagagccggt acaacccctt
    17461 tttcgtctga gccggccacg cacccgtccc ccgcagccat gccgcccgcg gggccgctct
    17521 ggtcgaaaaa tctttgccgg cgcgctacac tcatgcgagt tgctggaaca tacaccaaat
    17581 tgaggacggt accatgaaaa tgactcgact gttcaatggc cattctaccc ggagcgttat
    17641 gggctgggtg gcgatgatct gcgcggtctg cctggcggca accgccacga cggccctggc
    17701 cgacgacgaa gccgcgccca aagaaaaatg gatcagtctg tttaacggca agaacctcga
    17761 tggctggatc ccgaaaatag ccaaacatga gtgcggtgac aactacgcca acacattccg
    17821 cgtggaagac ggcattctca aggtgtgcta cgacggatac ggcccgtttg acgaccagtt
    17881 cgggcacctg ttctataaag aaccgttctc gaactaccgg ctgcgggtgg aataccggtt
    17941 tgtgggggaa cagatcgagg gcggggccgg ctgggcctac cggaacagcg gcatcatgat
    18001 ccatggccag acaccccaga gcatgggaaa agatcagagc ttcccggtgt ccattgaagt
    18061 gcagctgttg ggcggttccg gctcgggcga gcgcaccacc ggcaacctct gcacaccagg
    18121 cacgcacgtc gtgattgacg gcaaactcga aaagaagcac tgcatcaact cgtcctcaaa
    18181 gaccttccac ggcgatcagt gggtcaccgc cgaagttgag gttcgcggca ataccattaa
    18241 acacttcatc aacggagagc tggtcctgac ctactgcgac ccacagctcg acgatggcga
    18301 caaagacgcc cagaagctcc tcgaagcggg acaggcgcct ttgttgacca gcggcacgat
    18361 ctcgctccag tccgagagcc atcccgtcga gttccgcaag gtcgaaatcc tgaaattcga
    18421 cgaatagcaa cgccctgtct ttcgagatcc gaaaaaaaag accggaaacg cccttggggt
    18481 agcgtttccg gtcttttgct tacgacgccc tccggcgtta gaaggccgag atcacgcccg
    18541 cgaaggcccg ggtaaccgga aattcgccgg ggaaccgcac gaattcaaca tgcccgtcca
    18601 ggtacagcac gttgcttccc ccggggacgt ggttgaattc cgtcacatcg gtactgacca
    18661 ggtcccacat gatgggcagt tggctctgcg ccagcgccga tcccgcggga ttgttgatgt
    18721 cggtcacgaa gaatcgctcc accccctcac gaagccggta gagggtccgg gtgccgtaat
    18781 cattcacgta ggtgacgtcc tgctcgaacg cggcgggatt tccattggcc gactccgtca
    18841 gaacgttgaa gatcgcgagg ataaaattgg ttgacaccgt gtccggagtg ccgaatggtg
    18901 tccacccgaa cgcatcattc tcctctactc ccgcgttcat gtagtcttct ttcccctgga
    18961 gcgcccaagc cacatacata tacgaaatgg ccgatatggc gcacggatcg aggttgccgt
    19021 cggggtcgcc gttcacattg aaacggccgg cctcgaccag gcctgctgcc gatgaatccg
    19081 acggacacac catcacgttg ggatcggtca gatactcggg gatcacggac gggccgtcaa
    19141 agataaactc cgcgtcgccg cccagcgcgg ggctgactgg cgggttcggc aggccgcact
    19201 gcgatacgta cgacgccgga tcatccgaat ccgacagcga ggtataccgg agcatggacg
    19261 gcaatttctc gcccttcgct tcgttggcat acatcttgag catgagcccg atctgtttga
    19321 gattgttggc gcaggaagcc cggcgggccg actcgcgcgc gcgggccagc gccggcagca
    19381 aaatcgccgc caatataccg atgatggcaa tcaccaccag taattcaatc aaagtaaatc
    19441 ccacggtgcg tattcttttg tgcatgttcg tctcctctgc tgatctgttt gctgttgtgt
    19501 ttgcaaatcc cttcgcgact cgggtttaat atccgtctgt gccgcccgac acagtcgcga
    19561 aagtgccaga gagtacttga gaatcgggcc attgtcaatg ctgtaatggg taatcgctta
    19621 cctatacagg tagtattgtg ctagctgagg ggcaggcgca gccgccgact attgtgcgtg
    19681 aggaatgagc cggtctggcc gcctgaatca aggcgttgtt ctcaaccgga gcgggacgca
    19741 gtcctttccc ccgcgaccgt ttggactggt aagcgcttac atccggtctc cccggaaaat
    19801 ggcgtgaccg cgatcaaagc gtgcttggag ctgcggaaaa gagatggcaa tgccggctag
    19861 actgcaacag ggcgcgctga tactgctgct tctaatcctg ccggccgcat cgtccgcggc
    19921 ttcccgggag acgcgcggcc aatccaatgc ggcccgaccg cctcttcggg tggtcgtgac
    19981 ccccgatgag cttgtaaacg aacccgcctc tcctcttctc tacggtcatc ttatccagag
    20041 cggattcgga cgccaggtgg atggcctgtg ggcggagatg ctgtttaacc gcagttttga
    20101 agcagtcaca cccttcaagg aatccgtgtg gggatggctg catcgcagac ctgacgatga
    20161 cctgagcacc gaagattggt ggcattcggg ctatgaggaa acggattggt acatcgcccc
    20221 aggcaacccc gaggcccatc tgggctattc agcgtactgg gattttcggc acggggaacg
    20281 ttcggcgcat gtgaccaatc gcaacaatac ctctgcggcg atcctggcgc aggacggcct
    20341 ctggttgcgt aagggaatgt cataccgtct tcgtggttgt ttgaggcacg gggatttgat
    20401 ggatccggat acgccaccga tcaacatcac cgtgggcctg tacggggagg cccgaaccga
    20461 cagcccgctg gccaccgcca cactgcgcgg gatcgggagc acctggcagc aggttgaatg
    20521 cgaattgccc aacagcgatt acgacggccc ggccacgctg ggcatcaccg tccccccggg
    20581 catcacactt gccctggacg gcctctcgct gatgccctcg gacaacgtgc agggctggcg
    20641 gcgcaacgct gtcgaagcgc tgaagcgggt gcgtccacgg atcctgcgct ggccgggcgg
    20701 cagctctgcg tcgttttact tctggcgcga tggcattggc ccggcacacg aacgccggcc
    20761 ccgcgaaagc acctattggg gcggactgga aaataacgac gccgggactg ccgaatttgt
    20821 ggccttgtgc cgtgaaatcg gcgcggagcc gctgatctgc gtcaatgtcc tgactggcag
    20881 ccctgccgaa gccgcggact gggccgccta ctgcaatgct ccgccggagc acccccaggg
    20941 cgcgttgcgc gccttgcacg gtcacgccga gccgtttggc gtcacgtatt gggagatcgg
    21001 caacgaaccg taccggaaat acgatgccct cgaatacgcc cggcgctgcg tcgagttcgc
    21061 ccggacaatg aaagccgccg accccggtat tcaactgatc atggccgttt actggcgttt
    21121 tcatgccttt ttgccccaga tgctcgaaat tgccgggccc catatcgatt tcgtggcgga
    21181 ccgggagctt gacgaaagcc atctccgcgg cgtgctcgaa acgctgcgcg aatacaacgc
    21241 ccggacaggc cgcagcatcc ggctgtgcaa cac
//
LOCUS       DAOPBQ010000004        20575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_2717_length_20575_cov_3.730994, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000004 DAOPBQ010000000
VERSION     DAOPBQ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 20575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20575
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2717_length_20575_cov_3.730994"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..2721
                     /locus_tag="PKW60_00285"
     CDS             283..2721
                     /locus_tag="PKW60_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HPJ97894.1"
                     /translation="MPNHKEFARFGLDREKHVTPDQEDEPLVWLVERRNGAGQEGVFA
                     VELAGVTVQGGRHAGQESSPDENAASRLVASVFSGLSAGEGIELVYRCGKDVPFTWQI
                     VGHVSGQTQTDVISNAKRLYTSLCASMRTESAAYRFSPVRSADALSSLDVQYPWQRRI
                     QGQRVAIQKAGRNPMGFAPGSQLLKTGGRLVLPDLTPGQAAPCNRLVSLLRQSPTLIE
                     LRLTLAKVILDDDAVLKTSAALEWLLSGEEKEVIQNELASLGERKQVHIGNSCRYLNA
                     WLDHGWGVRLTATVASEHAVTPALLTMIADDLTHGKNTRIEDMPEESYETGMCTATGI
                     DFQDCLPAGRLLPSFFPAPAQLVSAQVRREYNREVPRLGTDGIILGRIRDGVRSTPVH
                     FRHHDRTRHVYILGSTGTGKSTLLANMIIQDIRNGHGVSVLDPHGDLYTQILENIPPE
                     RVDDLILINPCDTERAVGINFLEAADPSCRKEMHLLVNEMMSIFKRLYMRETQGPIFE
                     TYMRNAMLLVMENEIPGATLLDIPRLFEHDDFRNALLAKCHNRAVLDFWRRTAERANG
                     EYSLANMTAYVTSKLNMFIYNSTMRCIIGQAKSTIDFYQAINEGRIILVNLSKGLLGE
                     LDTQLLGMLITGKLFCAALNRASEPTATPRKSHFLYIDEFQNFTSGSVAQMLSEARKF
                     GLYLTLANQNLSQLREGHEASSLSETVLSNAGTLLCFRTGAPDAERIEQYTLPEFGSQ
                     DIQYMPDFHLIAKLLVNQCPSRPFVFETLPFETAPAGRGLKRSIENARKRYTVDIQEV
                     ERDIASRHAALQIL"
     gene            2790..3683
                     /locus_tag="PKW60_00290"
     CDS             2790..3683
                     /locus_tag="PKW60_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPJ97895.1"
                     /translation="MRCPDCGHNNPNNRTTCQICFTSLGSPTTTQGRGVNTLPTMPAS
                     PLDPPRRQASQRAPWAAAPSAYPDGTNPSRPYIVAAQTVAGAGPAPRTGELHGHADVT
                     GRVIAMDAPLLERPDFDVCRFLTRTLWLMLLVVSPLIFLHAVLVMLGAVPAILAVVGL
                     LFLARFLSPTNLFTMYHLLALVNPLGRRNENEQVPVRYLRVRGEDGETEYVVRMKGTL
                     TLSNIMPDDLVSFWGRWRRGVLHARYAFSHRTQAWVELQRSYTWVTLAITACVVLLLI
                     AYFYGPVSQLFVRAHEWGIVQ"
     gene            3722..6262
                     /locus_tag="PKW60_00295"
     CDS             3722..6262
                     /locus_tag="PKW60_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97896.1"
                     /translation="MLQGDHPDLETPVFDADPSQGEGLGLVRIEGLTRFWESAEAGHG
                     DLGARQAEFLAGLANAAVPWGMTILGEPGGVSIYLALTGGAAALQGWQEFLSAVFPGC
                     QLSAGPEARLVLNAYRHLPQIASLTGNPSTGDGDPRTAEPLLRLEPLIGAMSGRRWAY
                     LVLARPVSSSEILKMNQTLAREEQDIRSTYLRPGTVEENNNPLAQRYVSLLQAARQRM
                     EAGMQSGMWNVQAYLMAESHDALARGGQALAGSFAGRDSLPQPFRVRSCRLGSAQAGF
                     RPPSTVLTSAEAAALSQLPSQEFPGYALREAVTFSVSPPPAPDHPSVAVGTIVASGVR
                     TRNWFEVRVEDLAKHVFVAGVTGSGKTRTCQYLLSQLWGEHQIPWWVVEPSTKIEYRR
                     LAASETFRDLQIYTLGNETCAPLRLNPLQILPGVSVQTHIDALSSLFGASFAMVTPMP
                     DVLNKALHRVYTERGWDLLQGTNPRGHALETQPTLSDLILTLKDLIPELGYNADVTGN
                     LRAGLLTRVQNLTLGGKGALLNSGLSTDLDRLLEKPTVLELSAIGDDDSKAFVMASLL
                     LHLSEYCQVKGSSDGQLRHVTLIEEAHRLLRAAPETIASEYANPRGKAVEALCQMLVE
                     MRAYGEGLIVVDQVPAKLAPDAIKNTNLKIIHRLVAGDDRALVGTTMNLSEPQKRHLA
                     ALPSGQAVVYAEGRNAPYLVQIPDHAGLQGYGAAKITNEAVKRLMHGKIAPLPDSGQG
                     ARARQQLPRCQGCDRPGCETYRNIARQLLNKDYSGPFAHAVQEGPESIWAFGARVATE
                     IWGDKTRADAAYCVVLALLSLAGYESGIIENMRNRLGPILIRMRRMEA"
     gene            6259..6918
                     /locus_tag="PKW60_00300"
     CDS             6259..6918
                     /locus_tag="PKW60_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97897.1"
                     /translation="MTSANDEMAEINEPAVDRARELQMEHYLSETDRAAVEQLSERYR
                     ELVPPERLEAVRQQDTIFESDRDAFDARYERETGKRLEPGKRILGYSFGASESPRVAT
                     DYLEVPHVIFHERLHTLSAPGIERELGKGLDEGITEELAIDNLGAEPLEGDWESYSRE
                     RETARELRTLCGDEAIESAYFQGDTRALRACLDRELGPQGLERLREYLDGPTEEPEPR
                     R"
     gene            6930..7871
                     /locus_tag="PKW60_00305"
     CDS             6930..7871
                     /locus_tag="PKW60_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97898.1"
                     /translation="MKALAPLPRKAAEDDELANLALLIVPLLAITVAIFVLAVIFMVA
                     FVAGPPAGGVFWWSRLTQRYQISKAKKVTCALVGAGLLVSPLILMALLEPSGWHHDTN
                     SVLSAVWLGTVLGLCGTALYLAIEAYRQVFWQHRRNILAEQWKTPGVRHQLWKAQRAL
                     HGLEKAMARNEEANQKLMAQQKALEEKMGRMIETSDVRFYSAEKYRWQGIYEAMSTED
                     LKQRSESLNREIVQLPETDPRCMPLTLEAAVVRLEVQDRMLACGTYGSHRETLAEHGK
                     KTQMVRSLQQKLQAIQAKVDRERTVIQELRRTRPVVN"
     gene            7884..8330
                     /locus_tag="PKW60_00310"
     CDS             7884..8330
                     /locus_tag="PKW60_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97899.1"
                     /translation="MSITKSYKTEIIVPKPQLQAAAGNIQGTPCMEIMGLAVQKIAQE
                     RGGRVTNGYQDCTGKKHRCLLGLSTPAAPNGVGVQVGRDGKVSFVYDQQGANRAEMQA
                     ICNDLARAYATIAVLRIRSRHGYQVSVESETRVGDGTRVSTRAVRA"
     gene            8358..9017
                     /locus_tag="PKW60_00315"
     CDS             8358..9017
                     /locus_tag="PKW60_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HPJ97900.1"
                     /translation="MRIHRFLPQTEAEGPGRRACLWVQGCQRHCPGCFNQATWDPSAG
                     NVVAVDQLFAWITEAPNIEGVTFVGGEPFLQAEPLAVLGARCQTQGLSVVTFTGYTLG
                     ELMLANLPHWQALLKVTDLLLAGPYEADRQDFSRPWVGSRNQEFLFLTPRYRHLEKAL
                     ESIPNHLELRVGALGEISMNGLASEAEISDLQSSLAELGLVTKPKGASHHGKSHPHRN
                     R"
     gene            8986..9192
                     /locus_tag="PKW60_00320"
     CDS             8986..9192
                     /locus_tag="PKW60_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97901.1"
                     /translation="MAKVILTEIDEQGNVTSDLSGFEGNECVAEEERFRRDLAEYGLV
                     LSTRNQRRKPAENQVGGAEGHRIL"
     gene            9241..9516
                     /locus_tag="PKW60_00325"
     CDS             9241..9516
                     /locus_tag="PKW60_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97902.1"
                     /translation="MNEQVLILLVEAVLVFAVVLAGLRIMLGRATVPVLVGMALCSLS
                     TVFPNDPFARAISAVSCRFAIPILLLWGLFHLLKSPTGAPPRSQGMR"
     gene            9648..10394
                     /locus_tag="PKW60_00330"
     CDS             9648..10394
                     /locus_tag="PKW60_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97903.1"
                     /translation="MKPAIAIILATALVMFAILMGCDAAKGPVGTNIDTKLKELTTWT
                     PEAIAKDPLGFVTHATYAADDILLKLKAAQIGMAQQRRSTERKMAASEQDLAAVKALF
                     AEFRTAYQEADSQQTWPKTVHGTAYSETQLRNQVWALGQRYEACLAENKRLPEFHDRL
                     SSYAIELEQRLFDAQAARSEFARQREVLRMNTAMADLSKLEAQVRGLADTEGFLLANN
                     GTPTVNQLVEGLKHTVTEAEFTELLTMPLE"
     gene            10433..11512
                     /locus_tag="PKW60_00335"
     CDS             10433..11512
                     /locus_tag="PKW60_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97904.1"
                     /translation="MSNLVRVLVLGLMLVLVVAILRESGSSPRLRQRFSQFEDSFAPW
                     TGKEPVWTPERIAADSAGYARYAQERIRTLSEEFDANVRRVRSQVAELGAMAHENDAA
                     LKSAQSELTGMCKAYVRARDAGSWPVQLGGVTFDEDALKRAIAMAQWQVEQRENLKQR
                     IRQEYERKQMEAGRLELNISNIRLDQIALAGTAEELRVQDTIQNIQQLEDLVHAAADT
                     GTESFVHELMEAREVVLSDEQLDQLLKATVQEPPATPATSIPDSVPPSPASGLDEMDP
                     LESAPPAAATTFPKRTQAVASSPVDPSPAQTLTPMQKDALGIDYACPVPTVKLRKTPV
                     YDQPGLIRSDRKVPLLPSPSSRRFR"
     gene            11533..12411
                     /locus_tag="PKW60_00340"
     CDS             11533..12411
                     /locus_tag="PKW60_00340"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ97905.1"
                     /translation="MKNALMVKLGGTILFVTALAGCQTVPASNMPARFDSELATETDP
                     PSHAVNSDNAWRPQGCIMFPRSEINTVAERQFRLALGKACQQHGIPLIEDGGPVPAGT
                     TAFLQVLRIQQEMAASGPVQESGPYRWYACHVECSDRRNDWRPQPNTAKSLVTISRLL
                     TFDSDIIPSRSNDSNDTLWMLANEIAAVAANGMYIRQIFLDVICQMDASDVTRRGSEL
                     FMLQRYEQASLYLGEICRRYPDYQPAFSYLGACQAALGAYDLARATLTQTIRLAPDTE
                     EAHRAKQWLEMLSASP"
     gene            12724..14241
                     /locus_tag="PKW60_00345"
     CDS             12724..14241
                     /locus_tag="PKW60_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPJ97906.1"
                     /translation="MRSSQLMAYLIANFKSRLFAYDPWNGLQHLTPQHQFEPVRVGAG
                     AGYDAGLQNEVRDLEGALRHMDGILRRERVAFMLSGMDCAREEELRQNQPLINALRAW
                     ALDGQIMTSKSAVWLVTAKPGAAIDTSTLESVTLVRPKLAGEEERNEIVHAQVQRAGQ
                     PLTPDQERALTLATAGLNLHQTQTVLIKTFNLTRRFEMETLKHYKADYIRRSDVVEIE
                     EPTVGFSDVGGYENVKNMVRRTLIMAMQRPERARLAALPLPRGLLLFGPPGTGKTLFA
                     KALARETNLPFINMKTENLFTKWLGESGQRVKNAIQLVEQAAPALVFIDEIDRFGKRT
                     SGGDDGASQETGRVFAQMLEWLGDEGRKAIVVGTTNVPEHLDPAFLRPGRFSYVVPFL
                     YPDETARLQILRIHLGLEGTRPKPAMDQAAVQSVLHEIANKTNYYAGADLEELVIRAK
                     QNFFQGNDARLTAGHLSQAHRDYRVDANERQKMIQHYKQLGPAFANSMTLLEEVS"
     gene            14323..15207
                     /locus_tag="PKW60_00350"
     CDS             14323..15207
                     /locus_tag="PKW60_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97907.1"
                     /translation="MGFRDNDDIPVNRRAAAETERVRTPVTELRNAVLAPRAESSPPD
                     WFPAIAISVLVSFMLLGVVALYSLRSQNGGPDAGQNAGSHTLRGIHTQRKPENRSLAL
                     PSAPNRRNDLPDLTSDKKRVERGASQITTDSTHTPWQEHKKQSVQQAGRKPESGPAQP
                     DVTEQLDPQDSRQPEEQTSQRASIPTGEALVTPPGIAEQSGQREASLLQLDAPEQFGT
                     HSASQPQQQIPQRTPIPASDAATTRHITLGQSQQRNAESQASQTRQRSEIAPPERPRA
                     DSRVPLLPLPQNFSKRRN"
     gene            15894..17312
                     /locus_tag="PKW60_00355"
     CDS             15894..17312
                     /locus_tag="PKW60_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPJ97908.1"
                     /translation="MLVFADDYEDANGNIGGLQHIISQYLRRGGRDPEVLLASVAEIN
                     RYLPDDWRMTRTTHNFRVQIIDERSSPYLVGFSYRAPDGEILHIVRTVLAKDHFDSDD
                     ARGAGIPPARRPDRRDRIQHPKIVVPNLYYFRPCPEFSRTPLFARQFEKVLETPGDVQ
                     YHRIHGLTKAGLDPDHYLWIRWTVDGDVKFVDYPHQDYHGQNFWLIPSMTMDNARRHK
                     ENYERKLDEHGIDRTSPPPTRAAIARYEKEFKFSVENVVETDENSPVLQFIKGRGYSV
                     QACTTKNNEDTYFDDDKWNLHASGVSFRLRKQSDNTRITLKKRFPAQNKPGSSTYRRI
                     EEEVVISPSEEELLLKGKPIVALPYRLLAYVVPAFEPATPRLKQVLLVKTERKMYRLL
                     DDRLRGLELCFDCSTYWSADGKRRLGSDCELELESKGVPSKQLSDLAQWLEREFGWAR
                     SDASKYERGLQMLGIVPTQRKP"
     gene            complement(17360..19423)
                     /locus_tag="PKW60_00360"
     CDS             complement(17360..19423)
                     /locus_tag="PKW60_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPJ97909.1"
                     /translation="MWTEFERLVKAIEEEGNAFWGPNLAAACARLNGLLPENGKAARK
                     PTEVVASCESIMRLLTEIRVASGVPVRADSSGSGGKAPEPLSISGENEKRQYRVLVID
                     DHAETWKPVLEHALTQALQEAPLTGAGATVDFSNDGKKVQVNPSGRELMEVISSYDLV
                     LLDVYLSGDTNGLDVLEVIRKTYHQLPVVLWTSSMDAENPARASQSNGYLFKKTATMG
                     DLETAFKYWLPIGLNRRTTTLPDPFFDHVIQDFESRELAQDMTEWCLKQMDSFHALDG
                     TFFRYFTDHGGRHFVKVLNILRRLLEPFAAMKESPILPADEEDREKWFVLLYLAVITH
                     ELGMFPMRVHGAPELFSEVGQNYLNDVRTLHGLRAMVMLADDTNSYWPDDEGKKLAER
                     LTQLRFGEELRKMLAVVVGYHPRYFKSLKPGTFLKWPNKKDERPKPAKSSSPYLTAQP
                     NLFVKTLEVLHQAFSDPTTREHLRKLCALFRFADALDITASRNPAQFLCSASSLPITQ
                     YIENLKRQLCTELTIDNGQVTISLVADQPESKEVLTALEMTGELDKKYKETEIEALRQ
                     PWKEKSDFPLALHNRMNTWLKNFWGIIAGARVAYEYAQGLKEQGLISGQGNGYASTEK
                     GRRLAAVLGALCVIGDLQSEYEAISDAGLHEKITLGTVNWKGYNTDHLSILEKVLKGY
                     EDSSA"
     gene            20255..>20575
                     /locus_tag="PKW60_00365"
     CDS             20255..>20575
                     /locus_tag="PKW60_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97910.1"
                     /translation="MSEGMETAWSDDKMLLDRLPPLIWTAGAHKMDLKRFSNELSGGA
                     SALEPVQSAYYRIPAIDANLAQSTLSADAKAELTQSAYYCIPAADLNFDSSILSAMEC
                     PRSLS"
BASE COUNT         4671 a         5814 c         5950 g         4140 t
ORIGIN      
        1 gatccccccg gcagccgaga cctctgtgac gcccgagatc cccccggcag ccgaggaggt
       61 ccagagcgcc gaacagcccg attaagggct cggctccgcc aaaccggggc gaggcagatc
      121 gcccgacgtc tgatttctgg ccagaatacc tgcaaataat acccggtctg tatgccccct
      181 ccatctcctg gcgctctgca agggtgcgca ggcgcttgtc agatgctttt tgacttggaa
      241 gcctcggatt ttctccaaaa ccaacgtcac aggagcagcg tcatgccaaa tcacaaggaa
      301 tttgcacgct tcggactgga tcgtgaaaaa catgttaccc cggaccaaga agatgagccc
      361 ttggtgtggc tggtagaacg acgcaacggg gcagggcagg aaggtgtgtt tgcggttgaa
      421 ttggcaggtg ttacagtcca gggagggaga cacgccggtc aggaaagcag cccagacgaa
      481 aatgcagcat ctcggctggt cgcaagtgtg ttctcaggtt tgtctgctgg cgaaggtatc
      541 gaacttgtct accgttgcgg taaggacgtg ccgttcacct ggcagattgt tgggcatgtc
      601 tccggtcaaa cccaaacgga tgtcatctcc aacgcgaaaa ggctgtacac aagcctttgc
      661 gcgagcatgc ggactgagag tgcggcctat cgtttttcgc ctgttcggtc tgcggacgcg
      721 ctttcttcgc tcgatgtcca atatccttgg cagcgccgga tccaggggca gcgagtcgcc
      781 atccagaaag caggcagaaa cccgatgggt tttgcgcccg ggtcccagtt attgaaaaca
      841 ggcgggagac tcgtgcttcc tgacctcacg cctggccagg ccgcgccctg caatcgcctc
      901 gtctccctgc tgagacagag ccccacgctc attgagctcc ggctgacttt ggccaaggtg
      961 atccttgatg atgacgcggt gttgaagacg tcagccgcgc tggagtggct gcttagcgga
     1021 gaagaaaaag aggttataca aaatgagtta gccagtttgg gagagcggaa gcaggtgcac
     1081 atcggaaatt cctgtcggta tctcaacgcc tggcttgatc acggctgggg tgtgcgcttg
     1141 actgcaacgg tagcctctga acatgctgtg acccccgccc tcctcaccat gattgcagat
     1201 gacctgactc acggaaagaa tacccgtatc gaggacatgc cggaagagtc gtacgaaacg
     1261 gggatgtgta cggcaaccgg cattgacttt caggactgcc ttccggcggg ccgtcttctg
     1321 ccgtcgttct ttcccgcgcc tgcgcaactt gtgagcgcgc aggtgcgtcg cgagtacaac
     1381 cgggaggtac cacgcctggg aacggatggg attatcctcg gccgcattcg tgatggggtg
     1441 cgcagtactc cggtgcattt caggcaccac gaccgtaccc ggcacgtcta cattctcgga
     1501 agcactggta ctggcaaatc cactctgctg gccaacatga tcatccagga tatccgcaac
     1561 ggccacggcg tcagtgtatt ggacccgcac ggagatctct atacacaaat cctggagaac
     1621 attccgccgg aacgggtgga tgacctcatt ctcattaatc cgtgtgacac agagcgcgcg
     1681 gtcggcatta attttctcga agcagcagac cccagttgcc ggaaagaaat gcacctcctt
     1741 gtcaatgaga tgatgagtat tttcaagcga ttgtacatga gagaaacaca aggccccatt
     1801 ttcgaaacct atatgcgcaa tgccatgtta cttgtgatgg agaacgagat tcccggcgct
     1861 acgctgttgg atatccctcg attatttgaa cacgacgatt tccggaatgc gctgctcgcc
     1921 aaatgccaca accgggcggt actggatttc tggcggagaa ctgcggaacg ggctaatgga
     1981 gaatactcct tggcaaatat gacagcttat gtcacatcta aacttaatat gtttatatat
     2041 aattccacaa tgcgctgtat tattgggcag gcgaagagca cgattgattt ttatcaggca
     2101 ataaatgagg gccggataat tttggtgaac ctttccaaag gccttctcgg ggaactggac
     2161 actcaattgc ttggcatgct cattactggg aagctctttt gtgcggcctt gaaccgagcc
     2221 tcggagccga cggcgacccc ccgtaaatct cacttcctct acatagacga gtttcagaat
     2281 ttcacgagtg gaagtgttgc tcagatgttg agcgaggcga gaaaatttgg gctctacctg
     2341 accctggcca atcagaatct gagccagctg agggaaggcc acgaggcctc aagcctctcg
     2401 gagacggttc tgagtaatgc cggaacgctg ctctgcttca gaacaggtgc gccggacgca
     2461 gagcgcatcg aacaatacac gctgccggaa tttggctctc aggacattca gtatatgcct
     2521 gatttccacc ttattgccaa attgctcgtc aatcagtgcc ccagccgccc ctttgtcttc
     2581 gaaacgctgc cgttcgagac tgcgcccgct gggcgcggcc tgaagcgttc catagaaaac
     2641 gcgcgaaaac gctataccgt agatatccag gaagtggaga gagacattgc gagccggcac
     2701 gccgcgcttc agatcctgtg atacggcctt tgtcagttgg ttatgtcagt gaagaggccc
     2761 cgccaagcga agcacaaaag gagggaatca tgcgttgccc tgattgcgga cacaataatc
     2821 cgaacaaccg gaccacgtgc cagatctgct tcacttccct ggggtcgccg acaacgaccc
     2881 aaggcagggg cgtgaacacc ttgccgacca tgccggcatc tccgctggat cccccacgcc
     2941 ggcaggcctc acagcgtgct ccgtgggccg cggcgccttc cgcatacccg gacggcacga
     3001 acccaagccg gccgtacatc gtggcggcac aaacggtcgc gggtgcgggt cccgcgccac
     3061 ggaccgggga gttacacggg cacgcggacg tgacgggcag ggtcatcgcg atggatgcgc
     3121 ccttgctcga gcgtcctgat tttgacgtat gccgctttct gacgagaact ctttggctga
     3181 tgctactggt ggtatctcct ttgatattcc tgcacgccgt gcttgtgatg ctgggcgcgg
     3241 ttcccgccat cctggcggtg gtcgggctgc tgttcctggc acgatttctt tcgccgacca
     3301 acctgttcac gatgtaccac ctactggccc tggtcaatcc gctgggacgc cggaacgaga
     3361 acgagcaggt gccggtacgc tatttgcgtg tgcgcggcga ggacggggag acggagtacg
     3421 tggtacggat gaaaggcacc ctgacgctca gcaacatcat gccggatgac ctcgtctcat
     3481 tctgggggcg ctggaggcgc ggcgtcctgc atgcccggta tgcgttcagc caccgcacac
     3541 aagcctgggt cgaactccag cggtcctata cgtgggtgac cctggccata accgcatgcg
     3601 tggtactcct gttgattgcc tatttttacg ggccggtttc gcaactcttt gtccgtgctc
     3661 acgaatgggg gattgtacaa tgagctcata cgccacaagc cgaatacagc agaaaatcag
     3721 cgtgctgcaa ggggatcatc ccgacctcga aacgccggta ttcgacgcgg atccctcgca
     3781 gggggaggga ctgggcctcg tccgcattga gggtctgaca cgcttctggg agagtgccga
     3841 ggcaggacat ggtgacttgg gcgcgcggca ggcggagttc ctggccgggc tggccaatgc
     3901 ggccgtgccg tggggaatga caattctggg agaacctggc ggcgtcagta tttaccttgc
     3961 gctgacaggc ggcgcggcgg cgctccaggg atggcaggag ttcttgtcgg ccgtgttccc
     4021 gggctgtcag ctctcggcgg gaccggaggc gcgcctggtt ctcaatgcct atcgtcattt
     4081 gccgcagatt gcgtcactaa cgggcaatcc gtcgacgggg gatggcgatc cccggacagc
     4141 ggagcctctg ttgcgcctgg aaccccttat tggagcgatg tccggcaggc gttgggcata
     4201 cctggtgctg gcacgcccgg tatcttcatc tgaaatactg aaaatgaacc agacactggc
     4261 acgcgaggag caggatatca gaagcactta cctccgtccc ggaactgtgg aggaaaacaa
     4321 caatcctctg gcgcagcgat atgtctccct gcttcaagcc gcgaggcaga gaatggaagc
     4381 ggggatgcaa tcgggaatgt ggaacgttca ggcctacctc atggcagaga gccacgatgc
     4441 gctcgcacgg ggtggtcaag ccttggccgg cagcttcgcc gggagggaca gtctgcctca
     4501 accgttccgg gtgcggtcgt gccgcctcgg ttccgctcag gctggattcc gccccccgtc
     4561 aaccgtgttg acctccgcgg aagcagcggc actgtctcag ctgccttctc aggagttccc
     4621 cgggtacgcc ttacgggaag ccgtcacctt ttccgtgtct ccacctccgg ccccggacca
     4681 cccatcggtg gccgtaggca ccatcgtggc cagtggcgtc cgaaccagga attggtttga
     4741 ggtgcgtgtg gaagacttgg ccaaacacgt ctttgttgcc ggagtaacag gctccggaaa
     4801 gacccgcacc tgtcaatatt tgctctcgca gctctggggg gagcaccaga ttccctggtg
     4861 ggtggtcgaa ccgtcgacca agatcgaata ccgccgactg gcagcttccg aaacgttccg
     4921 agatctgcaa atctatactc tgggtaacga gacatgtgcg cccctgcgtc tgaatcctct
     4981 ccagattctg ccgggcgtat ctgtccagac gcacatcgac gctctgagct ccctgttcgg
     5041 cgcgtccttc gctatggtca cgcccatgcc ggacgtcctg aacaaggccc tccatcgcgt
     5101 ctataccgag cgtggctggg acttgctcca ggggacaaac ccgcgaggcc atgcgcttga
     5161 aacgcaacca accctatccg atttgatact gaccctgaaa gacctgatcc cggagttggg
     5221 ctacaacgcg gacgttacgg ggaatctcag ggcgggcttg ctgacgcgtg tgcaaaatct
     5281 gaccctgggg ggcaaaggtg cgttgctcaa cagcggtctc tccacagacc tggaccgcct
     5341 cttggaaaaa ccgacggtgc tcgagttgag cgccatcggt gacgacgaca gcaaggcctt
     5401 tgttatggcg tctctcctgc tccatctctc ggagtactgc caggttaaag gatcaagcga
     5461 tggacagttg cgccatgtca cactcattga agaggcgcac aggctcctgc gagcggcgcc
     5521 cgagaccatc gcctccgaat atgccaatcc gcgaggtaag gcagtcgaag ctctttgtca
     5581 gatgcttgta gaaatgcggg cctacgggga aggccttatc gttgtcgacc aggtaccggc
     5641 taaactcgcg cccgacgcga tcaaaaatac caacctcaag atcatccacc ggctggtggc
     5701 gggcgacgac agagccctgg tggggacgac aatgaatttg tcggagcccc agaagcgcca
     5761 tctggcggct ctgccgagcg gtcaggccgt ggtctatgcc gaaggccgca acgccccgta
     5821 cctggtgcag attccggatc atgcggggct gcaggggtac ggagccgcga agattacaaa
     5881 cgaagcggtc aagcgtctga tgcacggcaa gatcgcgccg cttccggatt ccggccaagg
     5941 tgcacgtgcg cgccagcagc tcccccgctg tcaggggtgc gaccggccgg ggtgcgaaac
     6001 atatcgcaac atcgcccggc aattgctcaa caaggactac agcggtccat tcgctcatgc
     6061 ggtgcaggag ggcccggaga gcatctgggc ttttggcgca agggttgcga ctgaaatctg
     6121 gggcgacaaa acgcgagcgg acgccgcgta ctgcgtggtt ctggcgctct tgtcccttgc
     6181 cggatacgag tctgggatca tcgagaacat gcgtaatcgc cttggcccaa tcctgataag
     6241 aatgaggagg atggaagcat gacatccgca aacgatgaaa tggcagagat caacgaacca
     6301 gcggtcgacc gggcgcgcga gctccagatg gaacactacc tgtcggagac cgatcgggct
     6361 gcggtcgagc agttgtccga gagatacagg gaattggtcc ctcctgaacg tctcgaggct
     6421 gtgcgccagc aggacaccat ctttgagagc gatcgcgacg cctttgacgc caggtacgaa
     6481 cgcgagacag ggaagagact cgagccaggc aaacgaatcc ttggctattc attcggagcg
     6541 agtgaaagcc cgcgagttgc caccgactac ctggaagtcc cccacgtaat ctttcacgag
     6601 cggctgcata cactctccgc cccgggcatc gaaagagagc tgggaaaggg tctggacgaa
     6661 ggcattacag aagagctggc aattgacaat ctgggggcgg aaccgctcga aggagactgg
     6721 gaaagctatt ccagggaacg ggaaacagca cgcgaattgc gaacgctctg cggtgacgag
     6781 gccatcgaaa gcgcctattt ccagggagac acccgggcgt tgcgggcctg cctggacagg
     6841 gaactcggac ctcaagggct cgagcggctg cgggaatacc tggatggtcc gacagaagaa
     6901 ccggagccac ggagatagaa aggtccgcca tgaaagcact tgcgccgctc ccacgaaaag
     6961 ctgccgaaga cgatgaactc gccaacctcg cccttctcat tgtgcctttg ctggccatca
     7021 cggtggccat ctttgtgttg gcggtaatct tcatggtggc cttcgtagcg ggtccacctg
     7081 ccggcggggt cttctggtgg tctcgcctta cccagcggta ccagatttca aaggccaaaa
     7141 aggtgacctg tgcgttagtt ggcgcgggcc tgctcgtgag tccgctaatc ctgatggcgc
     7201 tgcttgagcc gagcgggtgg catcatgaca cgaatagcgt gttgagcgcg gtgtggttgg
     7261 gaacggtgct gggactttgt ggaactgccc tttaccttgc aatcgaagcg taccggcagg
     7321 tgttctggca gcaccgcaga aacatactgg cggaacagtg gaagactccg ggcgtgcgtc
     7381 atcaattgtg gaaggcgcag cgcgctcttc atggcctgga gaaggcgatg gcccgcaacg
     7441 aagaggcaaa tcagaagctc atggcgcaac agaaggctct tgaggagaag atgggccgca
     7501 tgatagagac gagtgacgta cgcttctaca gcgccgagaa gtatcggtgg cagggcatct
     7561 atgaagctat gagcaccgaa gacctcaagc agcggagtga atcgctgaat cgggagatcg
     7621 tgcaactgcc cgaaaccgac ccgcgctgca tgccgcttac gcttgaggct gcagtggtgc
     7681 gactcgaggt acaggaccga atgctggcct gcggcacata tggctcgcac cgggaaacgc
     7741 tggcagagca cggaaaaaag acacaaatgg tcaggtcatt gcagcagaag ctgcaagcga
     7801 ttcaggccaa ggtggatagg gaacggacag taattcagga acttcggcgc actaggccgg
     7861 ttgtcaactg aaaggaaagt gcaatgtcca tcaccaaatc ttacaagacc gagatcatcg
     7921 tacccaagcc tcagcttcag gccgccgcag gtaacattca gggcacgcca tgcatggaga
     7981 tcatgggcct ggcagtccag aagatcgctc aggagcgtgg aggccgcgta accaacggtt
     8041 accaggactg taccggcaag aaacatcgtt gcctcctggg actgtccacg cccgccgccc
     8101 cgaacggtgt cggcgtccag gtgggccgcg atggcaaggt gtcttttgtt tacgaccagc
     8161 agggcgcgaa ccgggccgag atgcaagcca tctgtaatga cctggcgcgc gcatacgcga
     8221 ccattgcggt attgcggatc cgcagccggc acggatatca ggtgagcgtg gaaagcgaaa
     8281 cacgcgtggg cgatggcacc cgtgtgtcga cccgcgccgt gcgcgcgtaa gaaagggggg
     8341 agcacgtgaa tgggaccgtg cgcatccacc gattcctccc ccagaccgag gcggagggcc
     8401 cgggcagaag ggcatgcctc tgggtgcaag ggtgccagcg gcactgcccg ggctgcttca
     8461 atcaggccac ctgggatccg agtgccggaa acgtggttgc cgtcgatcag cttttcgcct
     8521 ggatcaccga ggccccgaac atcgaaggcg tcacctttgt gggtggggag cctttcctgc
     8581 aggccgagcc actcgccgtc ctgggggcac gatgccagac tcagggcctg tcggtcgtta
     8641 cgttcaccgg gtataccctg ggggaactga tgctggcgaa cttgccccac tggcaggcgc
     8701 tactgaaggt gacggatctg ctcctggcgg gaccttacga agcggacaga caggatttct
     8761 cccggccctg ggtgggcagc cgaaatcagg aattcctctt tttgacaccg cggtaccgcc
     8821 atttggaaaa agcactggaa agtatcccaa accatcttga attacgcgtc ggcgctcttg
     8881 gtgaaatcag tatgaacggc ttggcatcag aggcggagat aagcgacctg caaagcagcc
     8941 ttgcagaact cgggcttgtt acaaaaccaa agggagcatc acatcatggc aaaagtcatc
     9001 ctcacagaaa tcgatgaaca gggtaacgtc acctctgacc tcagcggttt cgaaggcaat
     9061 gaatgcgtgg ccgaggaaga gcgcttccgc cgggaccttg ccgaatacgg attggttctc
     9121 agcacccgga atcaacggcg gaagcccgcc gagaatcagg tcggcggcgc ggagggtcac
     9181 cgaatcctgt agggccgggg aacagcagcg ggcacattac ctgacagaga ggagcgcgag
     9241 atgaacgagc aagtactgat tctgctggta gaagcggtcc tggtgttcgc agtcgtcctg
     9301 gcaggtctcc ggatcatgct gggccgcgcg acagtccctg tccttgtcgg catggccctg
     9361 tgttcgcttt ctacggtgtt tccgaacgat ccgtttgccc gggcgatttc agcggtatcc
     9421 tgccggttcg ccattccgat acttctgtta tgggggctgt ttcacctgtt gaaaagcccg
     9481 actggcgcgc cgccgcgaag ccagggcatg cggtgagcct ctcgccggaa atctccgggc
     9541 agaaggacta aggccgcctc cccggtcatg ccagaattgg cacaatcgtt gcttactcga
     9601 taaacaccga tccacgaaca cacaaacgaa aaggaaggaa gctcgccatg aaacctgcca
     9661 tcgctatcat cctcgccacg gccctcgtga tgttcgcgat actcatgggc tgtgacgccg
     9721 caaaaggccc cgtgggcacg aacatcgaca ccaagctcaa ggaactgacg acctggacgc
     9781 ccgaggcgat cgccaaagac cccctggggt tcgtaaccca tgccacatac gcggccgatg
     9841 acatcttgct gaaactcaag gcggcacaga ttgggatggc ccagcaacgg cggagcacag
     9901 aacgcaaaat ggcggcaagc gagcaggact tggctgctgt aaaggcactg ttcgcggaat
     9961 ttcgtacggc gtatcaggaa gcagacagcc agcaaacctg gccgaaaacg gtccacggga
    10021 ccgcctattc cgaaacgcag ctgcgaaacc aggtttgggc tctgggacag cgctacgaag
    10081 cctgcctggc cgaaaacaaa cggcttcccg agtttcacga ccgcctgtcc tcctacgcaa
    10141 tcgaactgga gcagaggctc tttgatgccc aggccgccag aagtgaattc gcgcgccagc
    10201 gggaagtcct ccgcatgaac accgcgatgg ctgacctctc caagcttgaa gcacaggtga
    10261 gaggtctggc cgataccgag gggtttctgc tcgccaacaa cggcacgccc accgtcaacc
    10321 agctcgtgga aggcctcaag cacaccgtga cagaagcgga attcaccgag ttgctgacga
    10381 tgccgctgga gtaacctgga gccggcaccg ctgcgcagaa ggagggattg cgatgtcaaa
    10441 tctggttcgt gtgttggtgt tggggctgat gctcgtcctc gtggtggcga tattgcgcga
    10501 atccggctct tctccgcgcc tgcgacagag gttctcccag tttgaagatt cgttcgcccc
    10561 gtggaccggc aaggagcccg tgtggacgcc ggagcggatc gccgcggatt ccgcgggata
    10621 cgcgcgctat gcacaagagc gaatccgcac tctctctgaa gaatttgacg ccaatgtgcg
    10681 gcgtgttcga agccaggtgg cagaattggg cgccatggct cacgagaatg acgcggcctt
    10741 gaaatcggcg caaagcgaac tgacgggaat gtgcaaagcc tatgtgcgcg cccgggatgc
    10801 cggttcatgg ccggtacagc tgggaggggt gactttcgat gaggacgcgc ttaagcgcgc
    10861 aatcgcgatg gctcaatggc aagtggaaca gcgcgagaat ctgaagcagc gcatccggca
    10921 agaatatgaa cgcaaacaga tggaagcggg cagactggag ctgaacatct cgaacatcag
    10981 actggaccag attgccctgg caggaaccgc ggaggagttg cgggtccagg acaccataca
    11041 aaacatacag cagcttgagg atttggtgca tgccgccgcg gacacgggca ccgagagctt
    11101 tgtccatgag ctgatggaag ctcgtgaagt ggttttgagc gacgagcagc tggatcagct
    11161 gcttaaggct acagtgcagg aacctcccgc gacccctgcc acttccattc cagactctgt
    11221 tccgccttcc ccggcctctg gcctcgacga aatggatccc ctggagtctg ccccccctgc
    11281 cgctgctacg acattcccga aaaggaccca agccgttgca tcgtcgccgg ttgacccgtc
    11341 tccagcacag accctgactc caatgcagaa ggatgcgctg ggaatcgact atgcctgccc
    11401 ggttcccact gtgaaattac gaaaaactcc ggtctatgat cagccaggat tgattcgcag
    11461 cgatcgcaaa gtgcccttgc tgccctcacc aagtagccgg cggttccgtt gatgcaaagg
    11521 agatgtcgcg cgatgaagaa tgctctcatg gtgaagctcg gtggaaccat actattcgta
    11581 acagcgctcg cgggttgcca gaccgttcca gcaagcaata tgcccgcacg attcgatagc
    11641 gagctggcca cggaaacaga cccaccttct cacgcggtta attcggataa tgcctggcgc
    11701 cctcagggtt gcatcatgtt tccacgaagc gaaatcaata ccgttgctga gcgccaattc
    11761 agacttgccc tcggcaaggc gtgccaacag catgggatcc ccctcattga agatggcggc
    11821 cctgttcccg ccggaaccac agcctttctt caagtccttc ggatacagca ggaaatggcg
    11881 gcatcggggc ccgtgcaaga gtccgggcca tatcgctggt atgcatgcca tgtcgaatgc
    11941 agcgatcgca gaaacgactg gaggcctcag cccaacacag caaaaagcct cgtcaccatc
    12001 tcacgccttc ttacatttga ctcagacatc ataccctctc gctcaaatga ctcgaacgac
    12061 acgctatgga tgctcgccaa cgagattgca gcagtggccg cgaacggcat gtacatcagg
    12121 caaatcttcc ttgacgtaat ttgccagatg gacgcctctg atgttacccg tcgcggctca
    12181 gagttgttta tgctccagcg ttatgagcaa gctagcctct atttgggaga aatctgccgt
    12241 cgctatccgg attatcaacc cgcgttcagc tacctcgggg cctgccaagc cgcccttgga
    12301 gcgtacgatc tggcgcgagc cacattaaca caaaccatcc ggttagcgcc tgataccgaa
    12361 gaagcccatc gagcgaaaca atggctggaa atgttgtcgg cctctcctta acataaaaca
    12421 tgtagacagc tgatattgaa attacgcact cttcgggagc gcgcaaatac gtaaacgctt
    12481 tgtggcgggt tctgaccgca gaatcacaga caagggtgct ctgcgggcag gcgtgtaatc
    12541 gggaagtcag gattaggcca aggcaggcaa agacctgccc tgttactgcg tcatctggtg
    12601 catacaaaag aagagggagc gaaaaaaatg ggaatgcaat tggactttgg ctggattgag
    12661 gatgcgatgg ctaacgtaaa tagcagcgca tcaacggcgg tcgttgtcga gactgcagat
    12721 cccatgcgca gtagccaact gatggcctat ctgattgcga attttaaaag ccgcctgttc
    12781 gcgtacgatc cctggaacgg tcttcagcat ctgacgccac aacaccagtt tgagccggtc
    12841 cgagtcggcg caggcgcggg ctacgatgcg ggcctgcaga acgaggtccg cgatctcgag
    12901 ggcgccctcc ggcatatgga cggcatcctg cgccgggaac gggttgcctt catgctgagc
    12961 gggatggatt gcgcgcggga ggaagaactg cgacagaacc agcctttgat caatgcactc
    13021 agggcgtggg cccttgacgg gcagataatg acttccaaat ccgctgtctg gcttgttacg
    13081 gcgaaaccag gtgcggccat tgacaccagc accctcgaga gtgtgacatt ggtgcggccc
    13141 aaactggcgg gcgaagagga aaggaacgag attgtgcacg cgcaggtcca acgggccggg
    13201 cagcctctca ccccggatca ggaacgcgcc ctgactctgg ccaccgccgg gctgaacctg
    13261 catcagacgc aaacggtact cataaagacc tttaacctga ctcgccgctt cgagatggaa
    13321 acactaaagc actataaggc agactacatc cgccgctccg acgtggtgga gattgaggag
    13381 cccacggtgg gattctcgga cgtgggcggc tacgagaacg tcaaaaatat ggttcgccgc
    13441 acgctgatca tggccatgca aagaccggaa cgggcgcggc tggcggccct tccattgcct
    13501 cgcggcctgt tgctcttcgg ccctcccggc acgggcaaga ccctctttgc aaaagcactg
    13561 gcgagggaga ccaatctgcc gtttatcaac atgaaaaccg agaacctctt cacaaaatgg
    13621 cttggagaat cgggccaacg ggtgaagaac gccattcaac tcgtggaaca ggctgcgcca
    13681 gcgcttgtgt tcattgacga gatcgaccgc tttggaaagc gcacctcggg cggtgacgac
    13741 ggcgcctcgc aggaaacagg gcgcgtattc gcgcagatgc ttgagtggct cggtgatgaa
    13801 ggcaggaagg ccatcgtggt cggtacaacc aacgtgcccg agcacctgga tcccgcgttc
    13861 cttcggcctg gccggttcag ctatgtggtg ccgtttctct atccggacga aacggcgcga
    13921 ttgcagatcc tgcggataca cctggggctc gaaggcacca ggcccaaacc cgcaatggac
    13981 caggcggcag ttcaaagtgt cttgcatgag atcgccaaca agacgaatta ctatgctggt
    14041 gcagatcttg aggaattggt gatccgcgcc aaacagaatt tcttccaggg caatgacgct
    14101 cgcttaaccg ccgggcatct cagccaggcg caccgggatt accgcgtcga cgcgaacgag
    14161 cgacagaaga tgatccaaca ttacaagcag cttgggcctg ctttcgcgaa tagcatgaca
    14221 ttattggagg aagtaagcta atcaaaaacc gcctggcttt tccggactgt tggccggata
    14281 atatgatact cgaggtaatg caatacactg cgaggacatg aaatgggctt tcgggacaac
    14341 gatgacatcc ccgtcaatcg gcgtgccgca gccgaaacag agcgggtacg cactccagtc
    14401 accgagttgc gtaacgcggt cctggcgcca cgtgccgaat cctcgccccc ggactggttc
    14461 ccggcgatcg ccatttctgt gctggtttct tttatgctgc tcggtgtggt cgccctgtac
    14521 tccttgcgta gccaaaatgg cggacccgac gccgggcaaa atgccgggtc acacactttg
    14581 cgcggcattc atacacaacg caagccagaa aaccgttccc ttgccctgcc cagcgcaccg
    14641 aatcggagaa atgatctgcc ggatctcacc tctgacaaaa aaagagttga aagaggggcg
    14701 tcgcagataa ccacggacag cacgcacacc ccctggcagg agcacaaaaa gcagtctgtc
    14761 cagcaagccg gccggaaacc cgaatcaggc ccggcgcaac cagacgtcac agaacagctc
    14821 gatccccagg attcgcgcca gccggaagag caaacctctc aaagagcatc gatacctact
    14881 ggagaagcct tggttacgcc tcccggcatt gcagagcaaa gcggacagcg cgaggcaagc
    14941 ctgctgcaat tggacgcccc ggaacagttc ggtacccaca gtgccagtca gccacagcag
    15001 caaatccctc aaagaacgcc gattccagcc agcgatgccg cgactacacg gcacattact
    15061 ctcgggcaga gccagcagcg caatgcagaa tcccaagcgt cgcaaacaag gcaaaggagt
    15121 gaaatagcgc ctcccgaaag gcccagggca gattcacgcg ttcctttgct gcccttaccc
    15181 caaaatttca gcaaacgacg caattgacac gcagacactc agctacccgt tctggactcg
    15241 tcttttccac ggcaatctta acccccacgc aatccggcag cggttcatgg ctcagcctgc
    15301 ccctgagccg gcaagcctgc ctgcacgctt tgtaccgttc ctcagccgtt tctgcccggt
    15361 acgcgctgcg taacttgggc ggaggagatg cttgctaaat gaatgaacat ttgctatttt
    15421 gccttggtgg caaagagcaa atactactct gcgagcggaa ttgcttaggc ggattgacgc
    15481 tttaatgtcg accgcacgag ggacgtctgt atgatcggca tgcagcacac agatctttcc
    15541 gcaaaaaccg tccagaccgc tggctccgcg tacagaggat gcgtgcgatg ggaactcggc
    15601 gacgtgtgcg tacccgtgaa ccctatcatc tcgctccgta ctgccattcc gtccggcgcg
    15661 aaggccgaag ttgcgcagag aaacgattat tattggcgta gtacatttgt aggtcggcga
    15721 gggtgcctct ctcgcggcga tgctgtcgac gaagcgccgc agccatcagg acaggggaat
    15781 gtggaagaac atacgcagac tgcgcataaa cggccaagtt gttgtcggcg aaaagcccga
    15841 gctttcccgg cgggcgttgt gaatgcgcgc gaaacaaaag gaagaagaac ctcatgcttg
    15901 tttttgcaga cgactacgaa gacgcaaacg gaaatatagg cggacttcaa catataatca
    15961 gccagtatct gcggcgagga gggcgggacc ccgaggttct gcttgcctct gttgcggaaa
    16021 taaatcggta cctccctgat gactggcgga tgacacgcac cacgcacaat ttccgtgtac
    16081 aaatcattga cgaaaggtca tccccttatc tcgtcggatt ttcctataga gctcctgacg
    16141 gagagatact tcatattgtg cgcacggttc tggccaaaga ccattttgat tccgatgatg
    16201 caagaggagc gggcattcct ccagcaagaa ggcccgaccg aagagataga attcagcatc
    16261 cgaaaattgt cgtccccaac ctctactatt tccggccttg tcccgagttc tccaggaccc
    16321 ctctcttcgc gcgtcaattc gaaaaagtcc tggagacgcc gggggacgtc cagtatcatc
    16381 gtatccatgg gttaaccaaa gcgggccttg atccggacca ttacctttgg attcgttgga
    16441 cagttgatgg agatgtcaaa tttgtggact atcctcacca ggattaccac ggacagaact
    16501 tctggcttat cccatcgatg acgatggaca atgccaggcg acataaggaa aattatgaaa
    16561 ggaaattgga cgaacacggc atcgaccgaa cctctccgcc tcctacccgc gcggccatcg
    16621 ctcgttatga aaaagaattc aaattttctg ttgaaaacgt ggttgagaca gatgaaaaca
    16681 gccctgtcct gcaattcatc aaaggtcgag ggtattccgt gcaggcatgc acgacgaaga
    16741 ataatgaaga cacctatttt gatgatgaca aatggaatct acatgcaagc ggcgtctctt
    16801 tccgcctgag aaaacaatcc gacaatacca gaataacatt aaaaaagcgt tttcccgcgc
    16861 aaaataagcc gggaagttcc acgtaccggc gaatcgaaga agaagtagtc atttcacctt
    16921 cggaggaaga attgctgctc aagggcaagc cgatcgtcgc actgccctat cgtttgttag
    16981 cgtacgtggt tcctgccttc gaaccagcga caccgcgtct caaacaagtt ttattggtca
    17041 agacagagcg aaaaatgtat cggctgctag atgatagact tcgcggtctc gagttatgct
    17101 ttgactgttc gacgtactgg agcgcagatg gcaagaggag gctgggatcg gactgcgagc
    17161 ttgaactcga gagtaaaggt gtaccttcca aacagttgag tgatctggct cagtggcttg
    17221 agcgcgagtt cggatgggca cgttcagatg cctcaaagta tgagcggggg ttgcagatgc
    17281 tggggattgt tccaacgcag cggaaaccat gagagggcgc tgaacgagtc gaggaaacca
    17341 gggcgagtcc atctcaaatt caggcgcttg aatcctcgta ccccttcagc accttttcga
    17401 ggatggaaag gtggtctgta ttgtatcctt tccaattcac agtaccgagc gttatcttct
    17461 cgtgcagccc agcatccgag atggcttcat actcggattg gagatcgcca atcacgcaaa
    17521 gagcgccaag tacagcggca agtctgcgcc ccttttctgt tgaggcgtac ccattgccct
    17581 ggccagaaat caacccctgc tctttcaagc cctgtgcgta ttcataagca acccgtgctc
    17641 ctgcaatgat gccccagaaa tttttgagcc aggtgttcat acgattatga agcgccaagg
    17701 ggaaatcgct cttctctttc cagggctgcc gcagggcttc aatctcggtc tccttatatt
    17761 ttttgtccag ctcccctgtc atttctagcg cagtcagaac ttccttgctc tcgggttgat
    17821 cagccaccag acttatggtt acctggccat tgtcgatagt aagctcggtg cacagttgcc
    17881 tcttcaggtt ctcgatatac tgcgtgattg ggaggcttga cgcggaacac aggaattgag
    17941 ccggattccg ggatgctgtg atgtccaggg cgtcggcaaa tctgaacaag gcacacaact
    18001 tgcgcaggtg ttccctggtg gtggggtccg aaaaagcttg gtgcaagact tccagagtct
    18061 tcacaaagag attgggctgc gcagtcagat acggtgaaga tgattttgcc ggtttaggac
    18121 gctcgtcttt tttattgggc cactttagaa atgtccctgg cttgagtgat ttgaaatacc
    18181 ggggatgata gccgacgaca acggcaagca ttttgcggag ctcttctcca aaacgcaatt
    18241 gggttaaacg ctccgccagc ttctttcctt catcgtccgg ccagtaggaa ttcgtgtcat
    18301 ccgccagcat gaccattgcg cggaggccgt gaagggttcg gacatcattc aggtaatttt
    18361 gtccgacctc cgagaacaac tctggggctc cgtgcactcg catcggaaac atgcccaatt
    18421 cgtgcgtaat aacggccagg tacaggagca cgaaccactt ttcccgatct tcctcatccg
    18481 cagggagaat cgggctttcc ttcatcgcgg caaacggctc cagcagacgc ctcagtatat
    18541 tcagcacttt aacaaaatgc cggcctccat ggtcggtaaa atatcggaaa aacgttccat
    18601 ccagggcatg aaagctgtcc atttgcttca gacaccattc ggtcatatcc tgggccagct
    18661 ctcgagactc gaaatcctgg atgacgtgat caaagaaggg atcgggaaga gtcgtggtac
    18721 ggcggttcaa gccgataggg agccagtact tgaatgcagt ctctaaatct cccatggttg
    18781 cggttttttt gaacaggtag ccgttgcttt gggatgcccg ggccggattc tccgcatcca
    18841 tactggaagt ccacagcacg acaggaagct gatggtatgt tttccgaatg acctccagaa
    18901 catccagccc gttcgtgtcc cccgacaggt agacgtccag cagcacgagg tcgtagctcg
    18961 aaatgacctc cataagctcg cgcccgctgg ggtttacctg tacctttttg ccgtcgttgg
    19021 aaaaatcgac ggtcgcgccg gctcctgtca aaggggcctc ttgcagggcc tgtgtgaggg
    19081 cgtgttcaag cactggtttc cacgtctccg cgtggtcatc tatcaccagc acccgatatt
    19141 gccgtttctc gttttctccg ctgatagaca gaggctctgg tgctttcccg ccagaaccgc
    19201 tcgaatctgc gcgtacgggg acgccggaag cgacccggat ctccgtgagg agccgcatga
    19261 tgctctcaca ggatgccacc acctctgtag gttttctcgc agccttccca ttctctggca
    19321 gcaggccatt tagacgagca catgcggcgg cgagattcgg accccagaac gcattccctt
    19381 cctcctcaat cgccttaaca agccgctcga attccgtcca caaagacttg cggccgtacg
    19441 agtcaatgct ggcttcagcc gctgccagaa tgcggagtgg ggccagcatg tgatttgtga
    19501 tgtcgcgttt gccgccgtga gagaagggtc caatgtggtc aaggtagatc atcctggcct
    19561 gagcgcgccg gatggcgtcg agtggcacag gattagtcct cgcatcgcgc gagggcttca
    19621 gctcgccgag gagctcgccg agactctcag ggacagcgtc cggagcgcct tgaaaatact
    19681 cggccgacac gctggtccaa tccgggccgg agcgttttgg tttcccaaac ggctgccagg
    19741 cgattagctg gacattgccc gaagtcggca gggcggccac cgctcgcacc aaggcctcag
    19801 gatcgtaggg aacatccaaa gtaaccttac acggagtccg gggatttgtt ttggggacca
    19861 gagtatccag aaccttctgc aatgcctttt gtgtcacgtc atatttcggg catagagcct
    19921 ccgcagcact actgcacatt catcagttcc ttaaccatct atgctatgcg caatcaagta
    19981 ggtttggtca gatgctcaat atacgcacca ttatatatct gcgttttgca gacaagtcaa
    20041 atctgaaaat ccttcctggc gagatccccc cgagcacggt ttttccacgt accatagtga
    20101 ttgtcggcaa acccggaaaa ggacttgacc atggtgtcac gtcactgtaa aattacgagc
    20161 gatgtgatag gaactcttgc ctttaagcag aagtcggcgc tatgtcagaa cagccggcta
    20221 tctgctcact cttatgaaag gggggccttg acctatgagc gaaggcatgg aaaccgcctg
    20281 gagtgacgac aagatgttac tcgacaggct accgcctctc atttggaccg ctggggcgca
    20341 caaaatggat ttgaagcgtt tcagcaatga actgagtggg ggagcatctg cactcgagcc
    20401 cgtccagagc gcgtactacc gcatcccggc aatcgatgcc aatctggccc aatccactct
    20461 gtctgctgat gccaaagcag agctgaccca gagcgcgtac tattgcattc cagcagcgga
    20521 cttgaatttc gattcgtcca tcctgtctgc aatggagtgt ccgcgttcat tgtct
//
LOCUS       DAOPBQ010000005        20447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_2736_length_20447_cov_4.333660, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000005 DAOPBQ010000000
VERSION     DAOPBQ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 20447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20447
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2736_length_20447_cov_4.333660"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PKW60_00370"
     CDS             <1..482
                     /locus_tag="PKW60_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPJ97911.1"
                     /translation="LARKICFEIEGLSVHDMMPYVERPVGEALLEPHRSYSKVMQLVL
                     KTAVVSGMVHVTGGGITDNLPRVLPEGLAAEIDLSTWEALPIFRFLQEAGGVADAEML
                     RTFNMGAGFLLMVPEEEEQKVLKTLSQACEEPIVVGRIVAGDGKVHYTGAIRYATSN"
     gene            466..3426
                     /locus_tag="PKW60_00375"
     CDS             466..3426
                     /locus_tag="PKW60_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPJ97912.1"
                     /translation="MPHRIEIGMKAGLHDPAGNAVRQQLAEDLGICVGAVNVFDVYTI
                     NVQLSDDELERVRTGLFADPIIHDAAANASSGADYDYVIEVGFRPGVTDNAGKGAAEG
                     VADILGRNLAPEEAVYKSTLYAIKGDVSAQDCERIAREALANELIERWAIKSRAEMAS
                     LGGKSLLELPRVSHQSQTEVHRIDLELSDSELVQFSRDNMLALELHEMQAIQAYYRDP
                     AVRAARIEAGLPAEPTDIELEILAQTWSEHCKHKIFNALIEYTDESGRTRVIDSLFDT
                     YVKAATDDVAKRVDWLVSVFHDNAGLIRFDDDHHFALKGETHNSPSALDPYGGAMTGI
                     VGVNRDILGAGLGCRCILNTDVLCVASPWFDGEVPPRLFHPRRVLRGAHRGVQDGGNL
                     SGVPNVNGAVVFHERFLGKPLVFCATGGIIPRTVAGKPSHEKGARPGDLIVMCGGRIG
                     KDGIHGATFSSEELHEDSPATAVQIGDPITQKKMADFLLEARDLGLYTSITDNGAGGL
                     SSSVGEMARTPGGADIHLEKAPVKYAGLAPWEIFLSEAQERMTVSVPPDKLDAFLALS
                     RRRAVESSVLGEFNQSGRLQCFYNGKLIGSLDMAFLHDGVPKMKLQAVWQAPAREPEF
                     AVCDNDVLASLKAVLGRPNVCSKESLVRMYDHEAQAQSVLKQFQGPMHDGPGDAGVIR
                     PVPGSNRGIAVSCGICPKYSEFDTYWMMACAADEAVRNLIAVGAELGMIAGLDNFCWP
                     DPVQSEKTPDGTYKLAQLVRCCEALYDACTAYDIPLISGKDSMKNDYRIGEHQISIPP
                     TVLFTAAAIIRDVAKVVSMDFKKAGDIVYAVGETRNELGGSEYLAMKGQRGGQAPTVD
                     FARARKLYAALSSAIGRGLVASCHDCSDGGLGVALAESAFGGCLGLQADLAGLGQDNS
                     AVALFSESQSRFVVTVEPAQTAAFESLFAGLPLFRLGEVRADGLFRVTGRDGLEVRVP
                     VEELRAAWKSPMQW"
     gene            3482..4273
                     /locus_tag="PKW60_00380"
     CDS             3482..4273
                     /locus_tag="PKW60_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPJ97913.1"
                     /translation="MSVRAIVLTGFGINCDNETQGALSRAGAATDRIHLNDLLDDAPR
                     LDAYHLFAIPGGFSFGDDVASGKILANRLRHRLGGALQKWVDDGKLLIAINQGFQTLL
                     HMGVLPLFDGTFARTATLAPNDSGRFENRWVHLKTEPGTRCVWLKDIEGLPLPVRHSG
                     GKFVPQDGEVLRRLYAAGQVALRYVRPDGSPASGAFPWNPSGAIDDIAGICDPAGRIF
                     GLMPHPEAFVDRTNHPTWTRSSLPDEGAGLQIFRNAVEYARANLV"
     gene            complement(4299..5705)
                     /locus_tag="PKW60_00385"
     CDS             complement(4299..5705)
                     /locus_tag="PKW60_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97914.1"
                     /translation="MPKAFDVRCNAAALYFLPVKTRVPLKFGKETVTHLTCARAKVRV
                     ENAQGKSAEGWGETPISVTWVWPSTLPYEERESVLKELCRRLAEAWANFTVIGHPVEI
                     GHAFSETRLQDILRELNAERAGQEPVPWLAALVCSSAFDIALHDAYGILNNRPVYETY
                     GPEHMNFDLTHFLEPADGVDVSFEGKYPQDFFVLPRPSALPAWHLVGGVDPLVESELT
                     GTEPDDGYPVLLHDWIARDGLDCLKVKLRGNDAAWDYDRLVHVGRIAQEHGANWLTAD
                     FNCTVTEPAYVNTILDRLVCEHPRIYGMILYVEQPFPYDLEANRIDAHSVSARKPLFM
                     DESAHDWRLVRLGRSLGWTGVALKTCKTQTGALLSLCWAKAHGMTLMVQDLTNPMLAQ
                     VPHVLLAAHAGTIMGVETNSMQFYPEASLPEQQVHPGLYQRRGGCVDLSTVSGPGFGY
                     RLHEIVRTLPAPEVEAEA"
     gene            5955..8600
                     /locus_tag="PKW60_00390"
     CDS             5955..8600
                     /locus_tag="PKW60_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97915.1"
                     /translation="MEKGGHIASDASKRDALPAHAARLWVWGETFVLIPLHAFWILTR
                     STPFVAENTTSALIAALPLAVLALTSLAMQLPHARAAVQRAERLFHLTASAGFLILLA
                     YLVVRDPFNPALPITWPLVFWSVCLGFALLANLMSHERLRRAPSSEARPRWMQGIALA
                     AGAWAALAWLASGMSWAPYFWTVSLVFHAGMAVQSRRAPVVAEKLGREPGSAAALVEA
                     WFIAALMLAAFFRQLFLCEMAGRAELKYAGFVSIAANPLFVFGCAVALLAGRFRLVFI
                     THGCVAALLLFTAAETGWPVALVLGYGSAALYGASTRQGPLTWTLSLPSVVLMWGFGL
                     TGFMMAGLVVKYGMGLELAQRFRALAALMPVLYGAWLILWGTMSWRGGGDAGSGDIPA
                     VPSSNDKNGGFWAYAGAWAVMLVITAVLGFAAMWPPVFFGRLARIEVGEATGVCHAGY
                     SRSDEEYARLHALGVRLMRVDFHWSTVQKDPDTWDFSQFDSYLDAAEKHGIKVVALLV
                     FDNNAVETDEIGAKRRNYIAPRDVPLFLEYIRRTVTRYQARVYAWELWNEPDLARFWD
                     GPRDEMYDLIRKSAQTVRETCPGALLLGPAMAGAFGVYSAPGIEGLHAAGALELVGHP
                     TLHVYVSEPRCYYNEFFRVRNAAAKYGHPGKLWITELGDPDGGVYPWRGSREHLAAHA
                     IKAYTIATRVGIEKLLWYCYRDAKPELLQKEPWDSEGFFGLVHHDGSWKPAACAYSLF
                     AHHCSHSEMRADRVQVRGGLGARQLRTALYRREDGDSALVMWFEPGLRPGAQARVSID
                     LGDLERPALVHSITSGDIHPLLDDVIDVTETPTFLTFKAAELESPVRLRVAESPADAG
                     WLLLALGLVAASACRVIGAKPAGRI"
     gene            complement(8671..9363)
                     /locus_tag="PKW60_00395"
     CDS             complement(8671..9363)
                     /locus_tag="PKW60_00395"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPJ97916.1"
                     /translation="MEEQTCKLPLIGEKAPAFKAETTNGIVNFPEDYKGKWVILFSHP
                     ADFTPVCTTEFMTFASMAQDFKKLNCELLGLSIDSTFSHIAWLRTIKEKISFKDMKNI
                     EIGFPVISDLTMEVSKKYGMLQPSASNTQAVRAVFLIDPKATVRAILYYPMSNGRNMD
                     EIKRLLIAAQHSDAHGIATPANWQPGDDVIIPPPSSSDEANERVKKAGKDYECLDWFL
                     NLKKCPHKDVGA"
     gene            complement(9435..10898)
                     /locus_tag="PKW60_00400"
     CDS             complement(9435..10898)
                     /locus_tag="PKW60_00400"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HPJ97917.1"
                     /translation="MSTKKKLTTNAGAPVPHNNNVLTAGKRGPMLLQDVWYLEKLAQF
                     DREVIPERRMHAKGSGAYGTFTVTHDITRYTKASIFSQVGKKTELFARFSTVAGERGA
                     ADAERDIRGFAVKFYTDEGNWDLVGNNTPVFFLRDPLKFPDLNHVVKRDPRTNLRSPH
                     NNWDFWTLLPEALHQVTITMSDRGIPLSYRHMNGYGSHTFSMINAENQRFWVKFHLKT
                     QQGIKNLTDAEAEAVIGQCRESHQRDLYYSIEKGEFPRWTMHIQVVPEKDAAKFPFNP
                     FDLTKVWPHKDYPLVEVGVMELNLNPENYFAEVEQSAFNPVNIVPGIGFSPDRMLQGR
                     LFSYGDAQRYRLGVNHHLIPVNRPRCPFHSYHRDGAMRVDGNHGSLLGYEPNSYGEWQ
                     EQPEYDEPPLELDGAATHYDFREDDDDYYSQPGALFRLMNPDEQQRLFENTARAMGDA
                     PREIKIRHIGNCLKADKAYGSGVAAALNIPLSEVEKG"
     gene            complement(11014..11469)
                     /locus_tag="PKW60_00405"
     CDS             complement(11014..11469)
                     /locus_tag="PKW60_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPJ97918.1"
                     /translation="MTLPRDIVEARLAAFREACLQKRLRLTYQRIEVFRALASSEEHP
                     DVETIHRFVRKRIPTISPDTIYRNLKLLAEHGLVSAAGMSHERLRFDANVDPHHHFVC
                     VKCGRIRDFTSSDVDCTTFPVEAQAFGTPLSVHLEVKGLCSVCQKGQKS"
     gene            complement(11574..15488)
                     /locus_tag="PKW60_00410"
     CDS             complement(11574..15488)
                     /locus_tag="PKW60_00410"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPJ97919.1"
                     /translation="MRFLAGVLVFCSAVPYWGDVSAAAITIGFATTEKTVDAMGPHNR
                     AAWEVAQTLGDATLLHVDGETGFLDTAGETLDLSVFDVIWYHQGDDIARTALYSGAPL
                     TALRAYAGNGGAMLLSGGALAMVAPLGLERDTRPQRHQLENWRDPAAMVLVKPGHPAF
                     AGLQSDGALVWLSRGGCPAVADFYWGSPAEGMPLGNSPKGVHRPLVEYALDQGRVIVF
                     GWHWPDYADIESPHRANLLKLTGNLLTYLADRGAWQPVTIHSDFPGLAGQDQEGIAEP
                     RWRALQRAIEDLTHTFPEHYVRGGEFLEQFNALEAEHARLLQEAGPESIAPIAQRFEA
                     LQREALLANPLLDFDRLLVIRRRADRLGLPTNYSSNSDLPPTGYENSLLSLGLPGRDN
                     TAETVFVPEGDAFIGDLDLHYGAERLLLSTPNAEGRWGIAELDLATGTLTRLPLIDEP
                     DVHNYDACYLPDERIVFCSTAPFIGVPCVGGHSKVTNLYLRDHDGQIRRLTHDQDHNW
                     CPAILNNGRILYQRWEYADIAHTFMRILFHANPDGSQQMEYYGSNSFWPTAMFYARPI
                     PGHPTKIVTVVGGHHDAPRQGELVILDPARGRHEADGVVQRIPGYGKPVEPVILDGLI
                     GASWPRFLHPFPLNEHYFIVSCKPSKTALWGVYLVDVFDNFVLLHEEPGWALLEPTPW
                     RATTRPPVVPDLADPTTATASVVLADVYEGPGLEGVPRGAVRALRIAGYDFAFHGLGG
                     EPDRVGLDGPWDVRRILGTVPVEADGSAHFEIPACTPICVQPLDAEGKALALMRSWFT
                     AAPGEGLSCAGCHEKQNMTVPAHTRPLAARRGPSAITPWYGPARNFSFEREVQPVLDA
                     FCVSCHNSEAGATFDLTARPAEEVPSDFQMHFSPSYMQLRRWVHTPTLESDAHMLPPR
                     AFHADTSKLIQILRDDHYGVELSAEAWDRLITWIDLNAPFHGTWKEAVASIPAKQAAA
                     VHGAERRRELHRRYAGIDEDPEAIHPPAVLQGPEAPPPFPSPPLFSHPAASPEAAAPR
                     SAGEPVIETVEIAEGVTLELVRIEPGQFTLGDDNGYPNERPAQVHTIEGPFFMGRFEI
                     TNRQYACFDPAHDSGLETGEAYQFGDDERGFLLNRPEQPVVRVSWDEALAFCEWLSST
                     TGRRFSLPTEIQWEYACRAGSSTPLWYGGLDDDFSKAANLSDATHYSVYYPHSPRALP
                     PWRPADTRFNDGWRVSARVGSFGPNPWGLHDMHGNVAEWTLSSYLPNPADAGQTTTRK
                     VVRGGSWMDRPRRARSAFRLHYEASQCVHDVGFRVVCETAGS"
     gene            15868..16470
                     /locus_tag="PKW60_00415"
     CDS             15868..16470
                     /locus_tag="PKW60_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPJ97920.1"
                     /translation="MARKKLATELRQEQILAAALEIVSAKGLRGLNMGAVARRVGIVP
                     SALYRHFRDKEEILDGLLGLIEERLLAHVEAARQEAACPLTALEVLLKRHVGLIRENA
                     GIPQVVFSEEVYGANSQRKARVYAILTRYLGEVEDLFAEAQCAGLVDPDLAPGDLSVM
                     FLGLVQPAAILWHVSDGGFDVTRLTDRAWRVFTQAIRGGL"
     gene            16470..17633
                     /locus_tag="PKW60_00420"
     CDS             16470..17633
                     /locus_tag="PKW60_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPJ97921.1"
                     /translation="MKTSKKKKLIVVPLLAAVLVGAYVFSQRNASNGNGAVRISGNIE
                     ATDVECSFKVAGWVESRLVSEGETVHAGQPVAQLDSEDLAQEVAMRRAEVAGTAAKLA
                     ELEAGSRQEEVERARAAVELAEARLDELLAGSRSQEVKAARASVQRAGAELTRAETDY
                     NRLEQLFERNAVSAKERDHAYAAFATARAQLAEAHERLGLTQEGPRQEQIAQARAALS
                     EARAWFEQVASGPRKETIEQARAELARAEHALRLAETRLSYASISSPISGLVLQDHVE
                     AGEYVAPGAPVVTIGDLGNVWLRGYILETDLGRVKPGQPALITTDTYPGKAYEGVVSF
                     ISSEAEFTPKNVQTAKERVNLVYRIKIDIPNPALELKPGMPADAEIHIDGAAG"
     gene            17633..18571
                     /locus_tag="PKW60_00425"
     CDS             17633..18571
                     /locus_tag="PKW60_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ97922.1"
                     /translation="MTPIRTENLTRTFGAVTAVDALNLDVREGEIFGLVGPDGAGKTT
                     TMRLLTAIMAPTSGAAWVAGRHTVDEAEAIKEEIGYMSQRFGLYPDLTVLENIHFYAD
                     IYSVPRRGRAERIDELLAFSNLTPFKKRQAGRLSGGMKQKLGLACALIHTPRVLFLDE
                     PTNGVDPVSRRDFWRILYKLLRQKVTIFVSTAYLDEAERCSRIGLIHKGRLIAVGTPD
                     EVKGLMSGSVLELRCALPRKATELLRTHFPPNNVGLFGDRVHVATETPEAAADTITRV
                     LAGEGLVVTSLREVESSLEDVFISVLSHRKDVEESS"
     gene            18568..19539
                     /locus_tag="PKW60_00430"
     CDS             18568..19539
                     /locus_tag="PKW60_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ97923.1"
                     /translation="MNAGTEPAVVVRGLGRRFGAFRAVNNVTFDVPRGEIFGFLGPNG
                     AGKSTTIRMLCGILAPTEGEGRVAGCDLRTQAEEIKARIGYMSQKFSLYEDLTVEENI
                     DFYSGIYRIPAEKKQERKTWALEMAGLSEYRGMRTSLLSGGWKQRLSLGCAMLHEPPV
                     LFLDEPTSGVDPISRREFWDLIYELSSKGVTVFVTTHYMDEAEYCDRIGMIYRGELVA
                     MGTPEALKRETMSEDVLEVLCDRPSDAMEVLERVEVVQEAALFGNGLHVTVARASGGG
                     AAVHRALVEAGFVVRRVEVITPSMEDVFVSLVEARDRLAAAQGEVAV"
     gene            19536..>20447
                     /locus_tag="PKW60_00435"
     CDS             19536..>20447
                     /locus_tag="PKW60_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ97924.1"
                     /translation="MKFIRVKAVARKEFIHIVRDPRSLGMAIAIPMLLLFLFGYALAL
                     DVDRVPLMVWDQSQTPASREFLTYFTGSRYFSLQRYASGYPELERALDANEVLVGLVI
                     PRDFAERARTRAPVRVQAIVDGSDSNTATISMGYLNAISLAYSNQVLLEETRRITGKT
                     PAPPVDLRPRVWFNADLESRNYIIPGLTAVIMMVIAALLTSLTVAKEWEQGTMEQLIS
                     TPVKETELVVGKLLPYFAIGMLDVLLAVLMGAYVFEVPLRGSVPLLFGVSAVFLAGAL
                     SMGMLISIAAKSQLLASQLAMILTFLPS"
BASE COUNT         3727 a         5857 c         6844 g         4019 t
ORIGIN      
        1 gcctggcgcg gaagatctgt ttcgagatcg aagggctcag tgtccatgac atgatgccgt
       61 atgtggagcg gccggtgggg gaagccctgc ttgagcctca tcgcagctat tccaaagtga
      121 tgcagctcgt gttgaagacc gccgttgtga gcgggatggt tcatgtcacg gggggcggca
      181 tcaccgacaa cctgccgcgc gtcctgcccg aaggcctggc cgcagaaatc gacctgtcca
      241 cgtgggaagc cctgcccatt tttcgctttc ttcaagaggc cgggggagtg gccgacgcgg
      301 aaatgctgcg caccttcaac atgggcgcgg gctttctgct gatggtgccc gaagaggaag
      361 aacagaaagt gctcaagaca ctgtcccagg cttgcgaaga accgatagtc gtgggacgca
      421 tcgttgccgg ggatggaaaa gtacattaca caggggcgat ccgctatgcc acatcgaatt
      481 gaaatcggca tgaaagccgg actgcacgat ccggccggga acgcagtcag acagcagctc
      541 gcggaagatc tcggaatctg cgtcggtgcc gtcaatgtgt ttgacgtata caccattaat
      601 gtccaactca gcgacgatga attggaacgg gtgcgcaccg ggctcttcgc ggatcccatc
      661 attcacgatg cggcggccaa tgcgtcatca ggggccgatt acgattacgt tattgaagtg
      721 gggttccggc ccggtgtgac tgacaacgcc ggcaagggcg ctgcagaagg cgtcgcggac
      781 atcctggggc gtaacctggc tcctgaggaa gcggtctaca aatccacctt gtacgccatc
      841 aaaggcgatg ttagcgcgca ggattgcgaa cgtatcgcac gggaggccct ggccaatgaa
      901 ctgatcgagc gctgggcgat caagtcccgt gctgagatgg catcgttggg cggcaaatca
      961 ctcctcgagc tcccccgggt ctcacaccag agccagactg aagtgcaccg gattgacctt
     1021 gaactttcgg acagcgaact ggttcagttc agccgcgaca acatgctggc ccttgaactt
     1081 cacgaaatgc aggcgatcca ggcctattac cgcgatccgg ctgtccgcgc ggcacgtatc
     1141 gaggctggcc tgccggccga accgaccgat attgagctcg aaatcctggc ccagacctgg
     1201 tccgaacact gcaaacacaa aatcttcaac gccctgattg agtacaccga cgaatcgggc
     1261 cggacccggg ttatcgacag cctgttcgac acctatgtca aagcggccac cgacgacgtc
     1321 gccaaacgcg tggactggct cgtcagcgtc ttccacgata acgccggctt gatccggttt
     1381 gacgacgacc accactttgc gctgaaaggg gagactcata acagcccgtc ggcgcttgat
     1441 ccctatggcg gggcgatgac gggcatcgtg ggcgtcaacc gggacattct tggcgcaggg
     1501 ctggggtgcc ggtgcattct caacaccgac gtgctctgcg ttgcctcgcc ctggtttgac
     1561 ggcgaggtgc cgccgcgcct gttccatccg cggcgggtgt tgcgcggcgc acaccggggt
     1621 gtccaggacg gagggaacct gagcggcgtc cctaacgtca atggcgccgt tgtttttcac
     1681 gagcggtttc tcggcaagcc gctggttttt tgcgctaccg gcggcattat tccccgcact
     1741 gttgccggga agcccagcca tgaaaaaggt gcgcggcccg gcgacctgat tgtcatgtgc
     1801 ggcggtcgca ttggcaagga tggcattcac ggcgcgacgt tttcgtctga agaattgcac
     1861 gaggactccc ccgcgacggc cgtgcagatc ggcgatccca ttacgcagaa aaaaatggcc
     1921 gattttctgc tcgaagcccg ggatctgggc ctgtacacca gcatcaccga caatggggcc
     1981 ggggggctct cgtcgagcgt gggcgaaatg gctcgaacgc ccggcggcgc cgacatccat
     2041 ctcgaaaaag cccccgtcaa atacgccggt ttggccccgt gggagatttt cctctccgag
     2101 gcgcaggagc gcatgaccgt ctccgtgccg cccgacaagc tggacgcgtt tctcgcgctg
     2161 tcgcggcgcc gcgccgtcga atccagcgtg ctgggtgaat tcaaccagtc cggcaggctc
     2221 cagtgtttct ataacggcaa gctgattggt tcgctcgaca tggcctttct gcacgacggc
     2281 gtgccgaaga tgaagctcca ggctgtctgg caggcgcccg cccgtgaacc tgagttcgcc
     2341 gtatgcgaca acgacgtcct ggccagcctc aaagccgttc tcggcaggcc taatgtctgc
     2401 agcaaagaat cgcttgtgcg gatgtacgat cacgaggctc aggcacagag cgtcctcaaa
     2461 caattccagg gtccgatgca cgatggtccc ggcgatgcag gcgtcatacg ccccgtgccc
     2521 ggttccaacc ggggcattgc cgtctcgtgc ggaatctgcc cgaaatacag cgagtttgac
     2581 acctattgga tgatggcctg tgcggccgac gaagccgtgc gcaacctgat cgcagtcggg
     2641 gccgaactcg ggatgatcgc agggctcgac aatttctgct ggcccgaccc ggttcagagc
     2701 gaaaagaccc ccgacgggac ctataagctc gcgcagctcg tgcgctgctg cgaagcactg
     2761 tacgacgcct gcaccgcgta cgatatcccc ctcatcagcg gcaaagacag catgaagaac
     2821 gattaccgaa tcggcgagca ccagatctca atcccgccga cggtattgtt caccgccgcg
     2881 gccatcattc gcgacgtcgc caaggtggtc agcatggact tcaagaaggc tggggatatt
     2941 gtgtacgccg tcggcgagac ccgcaacgag ctgggcggca gcgaatatct tgccatgaaa
     3001 ggccagcggg gaggccaggc gcccacggtc gacttcgcgc gggcccgcaa actgtacgca
     3061 gcgctgtcgt ctgcgatagg gcgcggcctg gtggcctcgt gccacgattg ctccgatggc
     3121 ggattggggg tcgccctggc cgaatcggca tttggcggct gtctcggcct gcaggccgat
     3181 ctcgccggat tggggcagga caacagcgcc gtagcgctgt tcagcgaatc gcagagccgt
     3241 tttgtggtga cggtcgaacc cgcgcaaacg gccgcgttcg agtctctgtt cgccggattg
     3301 cccctctttc gccttggtga agtgcgtgcg gatggtctgt tccgggtgac cggccgggat
     3361 ggcctcgaag tccgcgtgcc ggttgaggaa ctcagagccg cctggaaatc gcccatgcag
     3421 tggtaatcac ggggacgccg tgctggaagg cgtcctcagc gtttcaagaa aggtgcagcc
     3481 agtgagtgta cgtgctatcg tcctgacggg attcgggatc aactgcgaca acgagacgca
     3541 gggcgccctc agtcgcgccg gcgccgccac cgaccgaatt cacctcaacg accttctcga
     3601 cgacgcgcca cgtctcgatg cgtatcacct gtttgccata ccgggcgggt tttcgttcgg
     3661 tgacgacgtg gcctccggca agatcctcgc caaccgcctt cgccacagac tgggcggcgc
     3721 gctccaaaaa tgggtggacg acggaaagct tctgatcgcc atcaaccagg gttttcagac
     3781 gctgctccac atgggagttc tgccgctttt cgacggaaca ttcgcccgga cggcgacact
     3841 ggcccccaat gattccggcc ggtttgaaaa ccgctgggtc catctcaaaa cagagccagg
     3901 gacccggtgc gtgtggctca aagatatcga aggcctgccg ttgcccgtcc gtcacagtgg
     3961 cggcaaattc gtgcctcaag acggcgaagt attgcgccgg ctgtacgccg cgggccaggt
     4021 ggctctcagg tacgtgcggc ccgacggatc tccagcttcc ggggcctttc cctggaaccc
     4081 cagcggggcg attgatgata tcgcgggcat ctgcgatccc gcaggccgca tctttggatt
     4141 gatgccgcat cccgaggcat ttgttgaccg taccaaccat ccgacatgga cccggtccag
     4201 cctgccggac gaaggcgcgg gcctccagat cttccgcaac gcggtcgaat acgcccgggc
     4261 gaatctagtc tgagcagggc tgtgcccctg ccgccgcatc acgcctccgc ttcgacctcg
     4321 ggtgcgggca gagtccgaac gatctcgtgc aggcggtagc caaatcccgg gccggatacg
     4381 gtcgacaggt ctacgcaacc tccccggcgc tggtacagtc cgggatgcac ctgctgctcc
     4441 ggtagcgacg cctccgggta aaactgcatg ctgttggtct ccacgcccat gatcgtgccc
     4501 gcatgagccg ccaggagcac gtgcggcacc tgcgccagca tgggattggt cagatcctga
     4561 accatcaggg tcattccatg agctttcgcc cagcacaggc tcagcagggc cccggtctga
     4621 gttttgcagg tcttgagggc cacgccggtc cagcccagcg agcgacccag gcgcaccagc
     4681 cgccagtcat gggcgctttc gtccataaac agtggtttcc gggcggaaac gctgtgcgcg
     4741 tcaatgcggt ttgcttccag atcgtaagga aatggctgtt ccacatacag gatcatcccg
     4801 taaatccgtg gatgctcgca caccaggcgg tccagaatgg tgttgacata ggccggctcg
     4861 gtcaccgtgc agttgaagtc ggcagtcaac cagtttgcac catgctcctg tgcaatcctg
     4921 ccgacatgta ccaggcgatc gtagtcccat gcggcgtcgt tgccgcggag tttgaccttc
     4981 aggcagtcga gcccgtcccg cgcgatccag tcgtgcagca aaaccgggta gccgtcgtcg
     5041 ggttccgtgc cggtcaattc gctctcgacc agcgggtcga ccccaccgac cagatgccag
     5101 gcaggcaagg cactgggacg cggcagaaca aagaaatcct ggggatattt cccttcaaat
     5161 gacacgtcaa ccccatccgc gggctccaga aagtgggtga ggtcgaaatt catgtgctcc
     5221 ggaccgtagg tttcatagac gggccggttg ttgaggattc cgtaggcatc gtgcaacgcg
     5281 atatcgaatg ccgaactgca gaccagcgcc gcgagccagg gcacgggctc ctgcccggcg
     5341 cgttcggcat tcaactcacg caggatgtcc tgcagacgcg tctcggagaa ggcgtgcccg
     5401 atctccacgg gatgcccgat gacggtaaaa tttgcccatg cttccgccag gcgccggcac
     5461 agttccttga ggacgctttc gcgttcctcg tagggcaacg tgcttggcca aacccaggtg
     5521 acgctgatgg gcgtttctcc ccagccttca gcactctttc cctgggcatt ttcgacgcgc
     5581 accttcgccc gcgcacaggt cagatgggtc acggtttctt tcccgaactt gagcgggacc
     5641 cgtgtcttaa ccggtaaaaa atacaatgca gcggcgttgc agcgaacatc gaaggctttg
     5701 ggcatcttac tgcttccttt ggcggttgaa ctcacgaaaa gccggccgtt ccggcgtcga
     5761 aatgcacccc cattgtaacg gctgtggcgg cgtactcaaa aagacgccca cgggccgggt
     5821 tatgcggccg tgcgaatact tgccggcctc ttgcgcattc ggccggggcc ctgtgataag
     5881 gtagccgctg ggccgatatg ttttcgcggt gggtatcgca ccagtgctgg aagcctgtgc
     5941 gtgcaggagg cgacatggag aaaggtggac acatcgcatc cgatgcgtcg aagagggatg
     6001 cgctgccggc acatgcggca cggctgtggg tgtgggggga aaccttcgtt ctgattccac
     6061 tgcatgcctt ctggatcctg acacgaagca cgccgtttgt ggccgaaaac accacgagcg
     6121 cgttgattgc ggcgcttcca cttgcggttc tcgcgctgac cagcctggcc atgcagctgc
     6181 ctcatgcacg agctgctgtc cagcgtgcgg agagactgtt tcatctgacg gcctcggcag
     6241 gatttctgat tctgctggca tacctcgtcg tgcgcgatcc ctttaacccg gcgctgccca
     6301 ttacctggcc gctggtgttc tggagtgtct gtctgggatt tgcgctgctg gcgaatttga
     6361 tgtcgcacga gcgccttcgg cgggcgcctt cgagcgaagc gcgtccgcgg tggatgcagg
     6421 gtatcgcctt ggcggcaggc gcctgggcgg cgctggcctg gctggcctcg ggcatgtcct
     6481 gggcgccgta tttttggacc gtgtcgcttg tatttcacgc cggtatggcg gttcagagcc
     6541 gtcgtgcccc ggtggtggcg gaaaagcttg gccgagagcc gggcagcgcg gccgcgctgg
     6601 ttgaggcctg gtttatagca gccctcatgc tggcggcctt tttccgtcag ctctttctct
     6661 gcgagatggc tgggcgggca gagttgaaat acgcgggttt tgtgagcatc gccgcgaatc
     6721 cgctgtttgt gtttggctgt gcagtggcgc ttttggcggg ccggttccga ctcgttttta
     6781 tcacacatgg ctgcgttgct gcgctcttgc tgttcacggc agcagagacg ggctggccgg
     6841 tagcgctggt gctgggttac ggttcagctg cgctgtacgg ggcttcaaca cgccagggcc
     6901 cgctgacgtg gacgttgagc ctgccgtccg tggtcctcat gtggggtttc ggcctgaccg
     6961 gattcatgat ggcggggttg gtggtcaaat acggcatggg actcgaactg gcgcagcggt
     7021 ttcgagcgct tgccgcgctg atgccggtgc tctacggcgc ttggctgata ctctggggca
     7081 cgatgtcgtg gagaggcggg ggagatgcgg gtagcgggga cataccggcg gtgccgtcat
     7141 caaacgataa gaatggaggc ttttgggcct atgccggggc gtgggcggtg atgttggtga
     7201 ttaccgccgt gctggggttc gcagccatgt ggccgcccgt atttttcggc cgcctcgccc
     7261 gtatcgaggt cggtgaagcc acgggcgtct gtcatgcggg gtacagtcgt tccgacgaag
     7321 aatacgcccg ccttcacgcg ctgggcgtgc gcctgatgcg ggtggacttt cactggagca
     7381 cggtgcagaa ggatcccgat acctgggatt tcagtcaatt cgacagctat ctcgacgccg
     7441 cggaaaagca cgggatcaaa gtggtcgcgc tgctggtctt tgacaacaac gccgtggaga
     7501 ccgacgagat cggcgcgaag cggcggaact acatcgcccc gagagacgtt ccgctcttcc
     7561 tggaatacat ccggcggacc gtcacccgct accaggcacg cgtctatgcc tgggaactct
     7621 ggaatgagcc ggacctggcg cgattctggg acggtccccg tgacgaaatg tatgacttga
     7681 tccggaaatc agcgcagacc gtgcgtgaaa cctgtcccgg cgcgcttctg ctgggtccgg
     7741 ccatggcggg ggcgttcggg gtatacagtg cgccgggcat tgaaggcctg catgcggccg
     7801 gggcgctcga gctggtggga catcccacgc tgcacgtgta cgtttccgaa ccgcggtgct
     7861 actacaacga gtttttccgg gtgcgcaatg cggcggcgaa atacgggcat ccggggaaac
     7921 tgtggatcac cgagctgggg gacccggatg gcggcgtgta tccgtggcga ggatcccggg
     7981 aacatctcgc ggcgcacgcc atcaaggcgt acaccatcgc caccagggtg gggatagaga
     8041 agctgctgtg gtattgctac cgcgacgcga aacccgaatt gctgcaaaag gagccctggg
     8101 attccgaggg ttttttcggg ctcgtgcatc atgatggttc gtggaagcca gcggcctgcg
     8161 cctatagcct gtttgcgcat cactgcagtc acagcgaaat gcgcgcagac cgggtccaag
     8221 tccggggagg actgggtgct cgccaattgc ggaccgcgct gtaccggcgg gaggacggcg
     8281 actcggcact ggtgatgtgg tttgagcccg gcctgcgccc cggcgcccag gcgcgggttt
     8341 ccattgacct gggcgatctc gaacgtccgg ccctcgtgca cagcatcacc agcggcgata
     8401 tccacccgtt gctggacgat gtgattgatg tgacggaaac acccacgttt ctgacattca
     8461 aggcggcgga actcgagtcg ccggtacggc ttcgtgtggc agagtcgccc gcggatgcgg
     8521 ggtggctgct gctggccctg ggcctcgtgg cggcaagcgc atgcagggtc atcggagcca
     8581 aacccgccgg gcggatctaa cgtgtatggc gtggcaaaaa agagaggcgg acggcccgca
     8641 ggccgcccgc ctctcacgac gacagggtga ctaggcgccc acgtccttat gggggcattt
     8701 cttcaggttg aggaaccagt caaggcattc gtagtcctta cccgccttct tcacgcgctc
     8761 attggcttcg tcgctggaac tcggcggcgg gatgatcacg tcatctccgg gttgccagtt
     8821 ggccggcgtg gcaatgccat gcgcgtcgga atgctgcgct gcgatgagca ggcgcttgat
     8881 ctcgtccatg ttgcggccgt ttgacatggg gtagtacaga atggcccgaa cggtcgcttt
     8941 tggatcgatc aggaataccg cacgcaccgc ctgggtgttg ctggcggagg gttgcagcat
     9001 cccgtatttt ttcgacactt ccatggtgag gtcgctgatc acggggaaac cgatctcgat
     9061 gttcttcatg tctttgaacg agatcttctc cttgatcgtg cgcagccacg cgatgtggct
     9121 gaaggtgctg tcgatcgaga gcccgagcag ttcgcagttg agcttcttga aatcctgcgc
     9181 catgctggcg aaggtcatga attccgtcgt gcagacggga gtgaaatcgg ccggatgcga
     9241 gaacagaatt acccacttcc ccttgtagtc ttcgggaaag ttgacgattc cattggtcgt
     9301 ctccgcttta aatgccggcg ctttttcacc gatgagcggc agtttgcagg tttgttcttc
     9361 catgagtctc tccttctctc tctttacgtt tgcagggttt tcacgggcgt ggggcgccgc
     9421 gtgcagccct tcttttagcc tttttccacc tcgctgagcg ggatgttcaa cgctgcggca
     9481 acgccgctgc cgtaggcctt atcggctttg aggcagttgc cgatatggcg gatcttgatt
     9541 tcccggggcg catcgcccat ggcgcgggcg gtgttttcaa acagacgctg ctgctcgtct
     9601 ggattcatca ggcggaacag cgctccgggc tgcgagtagt agtcgtcatc gtcttcacgg
     9661 aaatcgtagt gggtggccgc accgtcgagc tcgaggggcg gttcatcgta ttcgggctgt
     9721 tcctgccatt cgccgtagct gttcggctcg tagcccagca ggctgccatg attgccgtcg
     9781 acccgcatgg cgccatcgcg gtggtagctg tggaacgggc agcgcggacg gttgacgggg
     9841 atcaggtggt gattgacgcc caggcggtag cgctgcgcgt cgccgtagga gaagagccgg
     9901 ccctggagca ttcggtcggg ggaaaatccg atacccggca cgatgttgac gggattgaac
     9961 gccgactgtt ccacttctgc gaagtagttc tccggattca ggttcagctc catcacgccg
    10021 acttcgacca gcggatagtc cttgtgcggc cagaccttgg tcagatcgaa aggattgaac
    10081 gggaatttgg ctgcgtcttt ctcgggaacc acctggatgt gcatggtcca gcggggaaac
    10141 tcgcctttct cgatgctgta gtacaaatcg cgctggtggc tttcacggca ttggccgatc
    10201 accgcttccg cttcggcgtc ggtgaggttt ttgatcccct gctgggtctt gagatggaac
    10261 ttgacccaga accgttgatt ttcggcgttg atcatgctga acgtgtggct gccgtagccg
    10321 ttcatgtggc ggtacgacag tgggatgccg cggtcgctca tggtgatggt cacctgatgc
    10381 aacgcttcgg gcagcagggt ccagaaatcc cagttgttgt gcgggctgcg caggttggtt
    10441 cgcggatcgc gtttgaccac gtggttcaga tcggggaatt tgaggggatc ccgcaggaag
    10501 aagaccggcg tgttgttgcc caccagatcc cagttgcctt cgtccgtgta gaactttacg
    10561 gcgaacccgc ggatgtcgcg ttccgcgtcg gcggcgccac gctcgccggc gaccgtcgaa
    10621 aagcgggcaa agagctcggt cttcttgccg acttgcgaga agatgctcgc cttggtgtag
    10681 cgggtgatgt catgggtgac ggtgaatgtg ccgtaggcgc cggagccttt ggcatgcatg
    10741 cggcgctcag ggatgacttc ccggtcaaac tgggcgagct tttcgaggta ccacacatcc
    10801 tgcaacagca tgggcccccg cttcccggcg gtcaggacgt tgttgttgtg aggcacaggg
    10861 gcgccggcat tggtagtgag ttttttcttc gtactcatgt cgcgtctcct ctccattttc
    10921 gttgcctggg gcacagactc cagtgttttc gtgaatcgcc aaacggtccc tgagcttcgc
    10981 ggcggtctat gccgaaaaac agggttgaat gcgctaactt ttctgtcctt tctggcacac
    11041 ggaacagagc cccttcactt caagatgcac cgacaggggt gtgccgaatg cctgagcctc
    11101 tacgggaaac gtggtgcaat cgacgtcgct cgaggtgaaa tcgcgaatgc gcccgcactt
    11161 tacacagacg aaatgatggt gcggatccac gttcgcatca aagcgcagcc gctcgtgact
    11221 catcccggcc gccgacacga ggccatgctc cgccagcagc ttcaggttgc ggtagatcgt
    11281 gtcgggtgag atggtcggaa tgcgcttgcg cacaaagcgg tgaatggtct cgacgtcagg
    11341 gtgttcctcg ctcgaagcga gtgcacggaa gacctcgata cgctggtacg tgagccggag
    11401 gcgcttctga aggcaggcct cgcggaacgc ggccaggcgg gcttcgacaa tgtcacgcgg
    11461 gagggtcatg cgccagtcct tttccccaag caattcgtag ataggattct atcctattta
    11521 aaaaatcgtg tcaagaactc tttttcaacc cggtttcagg gcgggtttcg gccttatgaa
    11581 cctgcggttt cgcacacgac gcggaagcca acgtcgtgta cgcactgtga ggcttcataa
    11641 tgcagacgga aggcggaacg ggcccggcgg ggccgatcca tccaggagcc gccgcggacc
    11701 actttgcgtg tggtggtctg gccggcgtcg gcggggttgg gcaggtagct ggagagggtc
    11761 cattccgcga cgttgccgtg catgtcgtgc agtccccagg ggttggggcc aaacgagccc
    11821 acgcgtgcag agacgcgcca gccatcattg aaacgggtgt cggcgggacg ccaggggggc
    11881 agggcgcgag gggaatgggg gtagtacacg ctgtagtgag tcgcgtcgga gaggttggcg
    11941 gctttgctga agtcgtcatc caggccgcca taccacaggg gcgtgctgct gccggcccgg
    12001 caggcgtatt cccactgaat ttccgtgggc agagagaagc gccgcccggt agtggacgag
    12061 agccattcgc aaaaagcgag agcctcgtcc caggacacac ggaccacggg ctgttcgggg
    12121 cggttgagga ggaaaccgcg ctcgtcgtcg ccaaattgat aggcctcgcc tgtttccagg
    12181 ccgctgtcgt gggcgggatc gaagcaggcg tattgccggt tggtgatttc aaagcggccc
    12241 atgaaaaacg gcccttcaat cgtgtggacc tgcgcaggcc gctcgttggg gtagccattg
    12301 tcgtcgccca gggtgaactg tcccggctca atgcggacga gttcgagcgt gacgccctcc
    12361 gcaatctcca cagtctcaat aacgggttcg ccggcgctcc ggggcgccgc ggcttctggc
    12421 gaggcggccg ggtgggagaa gagcggcggg ctgggaaacg gcgggggcgc ctccggtccc
    12481 tgaagcactg cgggaggatg gatagcctcg ggatcctcgt cgatacccgc gtagcgacgg
    12541 tgcagctcac gcctccgctc cgcgccgtgc acggcggcgg cctgcttggc gggaatggat
    12601 gcgacggctt ccttccaggt gccgtgaaac ggtgcgttca aatcgatcca ggtgatcagg
    12661 cgatcccagg cctcggcgct gagttccacg ccgtaatggt cgtcgcggag gatctggatc
    12721 aatttgctcg tgtcggcgtg aaaggcgcgg ggtgggagca tgtgggcgtc gctttcgagg
    12781 gtgggcgtat gcacccatcg gcgaagctgc atgtacgaag gagagaaatg catctggaaa
    12841 tctgagggca cctcttcggc gggccgggcg gtgagatcaa aggtggcgcc ggcttcgctg
    12901 ttgtggcagc tgacgcaaaa cgcgtcgagc acgggctgca cctcgcgttc gaaggagaaa
    12961 ttccgggccg ggccatacca cggggtgatg gcggacggcc cgcggcgtgc agccagtggc
    13021 cgggtgtgtg cgggcacggt catgttctgt ttttcgtggc agccggcgca cgacaggccc
    13081 tcgccgggag cggccgtgaa ccagctccgc atgagggcaa gggccttgcc ttcggcatcc
    13141 agaggctgca cgcaaatggg agtgcaggcg ggtatctcga aatgagccga gccgtcggcc
    13201 tcgacaggaa cggttccgag gattcgacgc acatcccagg gcccgtccag gccgacccgg
    13261 tcgggttcgc cgccgagtcc gtggaaggcg aagtcgtagc cggctatgcg gagggcacgg
    13321 acagcgccgc ggggcactcc ttcgagccca ggcccctcgt aaacatcggc cagtacgacc
    13381 gacgcggtgg ccgttgtggg atcggccagg tcagggacaa ccggcggacg cgttgtcgcg
    13441 cgccaggggg tgggttcgag cagggcccag ccgggttcct cgtgcagcag aacaaaatta
    13501 tcgaaaacat ccaccagata gacgccccac agagcggtct tcgacggttt gcaggagacg
    13561 atgaagtagt gctcgttgag cgggaagggg tggaggaaac gcggccagct cgcgccgatg
    13621 agtccgtcca ggatgactgg ttcgacgggc ttgccgtagc cggggatgcg ctggaccacg
    13681 ccatcggcct cgtggcgccc acgcgcggga tccaggatga cgagttcgcc ctggcggggg
    13741 gcgtcgtgat gtccgccgac gaccgtgacg attttcgtcg ggtggccggg gatcgggcgc
    13801 gcgtagaaca tggccgtggg ccagaatgaa ttgctgccgt aatattccat ttgctggctg
    13861 ccgtcggggt tggcgtgaaa aaggatccgc atgaaggtgt gtgcgatgtc tgcgtactcc
    13921 cagcgttgat acagaatgcg gccgttgttg aggatcgccg ggcaccagtt gtggtcctgg
    13981 tcgtgcgtga gccggcggat ctggccgtcg tggtcgcgga ggtacaagtt ggtcactttg
    14041 gaatggccgc cgacgcacgg cacgccgatg aacggcgcgg tcgaacagaa cacgatgcgt
    14101 tcgtcgggca gatagcaggc gtcgtagttg tgcacgtcgg gttcgtcgat gagtgggagg
    14161 cgcgtgagcg tccccgtggc caggtcgagt tctgcgatgc cccaccgccc ctccgcgttg
    14221 ggagtggata agagcaggcg ctcggcgccg taatgcaggt cgaggtcgcc gatgaacgcg
    14281 tcgccctcgg gcacgaacac ggtctcggcg gtgttgtccc ggccggggag gccaaggctg
    14341 agcagggaat tttcgtagcc cgtgggcggc aaatcagaat tactgctgta attggtgggc
    14401 aatcccagac ggtcggcacg gcggcggatc acgaggagcc ggtcgaaatc gagcaggggg
    14461 ttggcgagca aggcctcgcg ctggagcgct tcgaaacgtt gtgcgatggg ggcgatgctc
    14521 tcaggcccgg cctcctggag gagccgggcg tgctcggcct ccagtgcgtt gaactgttca
    14581 aggaactctc ctccgcgcac atagtgttcc ggaaaagtgt gcgtgagatc ctcaatcgcc
    14641 cgctggaggg cgcgccagcg cggctcggcg atgccctctt gatcctgccc ggccagcccg
    14701 gggaaatctg agtgaatggt cacaggctgc caggcgccgc ggtcggccag ataggtgagc
    14761 agattgccgg taagtttcaa aagattggca cgatgggggc tttcgatgtc agcgtaatcg
    14821 ggccagtgcc atccgaatac aatgacgcgg ccctgatcca gcgcatattc aacgagtggc
    14881 cggtgaacgc ctttcgggct gttgcccagg ggcattcctt cggcgggact gccccagtaa
    14941 aagtccgcga ccgcagggca gccgcctcga ctcaaccaga ccagagcgcc gtcggattgc
    15001 agcccggcaa atgcggggtg cccgggcttg accagcacca tcgccgcggg gtcgcgccag
    15061 ttttccagtt ggtggcgctg cgggcgggta tcgcgctcca ggccaagcgg cgccaccata
    15121 gccagggccc cccctgaaag caacatcgcg ccgccgttgc cggcatacgc gcgcagggct
    15181 gtcaacgggg cgccgctgta cagggcagtg cgcgcgatat cgtcgccctg gtggtaccag
    15241 atcacgtcga acacggacag gtcgagggtc tctccggcag tgtcaagaaa gcccgtttcg
    15301 ccgtccacgt gcagcagggt cgcgtcgccc agcgtttgcg cgacttccca cgccgcccgg
    15361 ttgtgcggtc ccatcgcatc taccgtcttt tcagttgtgg cgaacccgat cgtgatggcc
    15421 gcggcagaca cgtcccccca atacggcacg gcactgcaga acaccagaac cccggcaaga
    15481 aaacgcatgc gcattcctta attggccaat gctggccatc tccatggcga acggctcgtg
    15541 gtatgcccga agggagtata ccgggaaacg gaaccggatt cagcccgcgc cagagggagg
    15601 catgatgtgc gggcgtgcgg aggatgccgc ctctgtctgg catgcgccct cgatccttcg
    15661 gggatcttat acatgaaagc gaatttatat tcacaaaaac ttgaaatgca cgggaaattt
    15721 agtaaaaaaa tcaggatcgt ctttaaattg caataaaata aggtatttaa ggttcttttg
    15781 ctgcagtatg ccagcctgtg ttgcgctgct tgacaggcgg aaggcgctgg gttataatgt
    15841 gaatgcgtat tcacatagga gggcggtatg gccagaaaaa aactcgcgac ggagctgcgg
    15901 caggagcaaa tcctcgccgc tgccctggag atcgtatccg ccaaaggttt gagaggcctg
    15961 aacatgggcg ctgtggcgcg gcgggtcggc attgtaccgt ctgccctgta tcggcatttc
    16021 cgggacaagg aagaaatttt ggatgggctc ctggggctga tcgaagagcg gctcctggcg
    16081 catgtggagg cagccaggca agaggcagcg tgtccgttga cggcgctcga ggttttgttg
    16141 aagcggcatg tcggattaat ccgggaaaac gcaggaattc cgcaggtggt gttttcggaa
    16201 gaggtctacg gggctaattc acaacggaaa gcgcgggtct acgcgatcct gactcggtat
    16261 ctgggagaag tagaagacct cttcgcggag gcgcagtgcg cgggactggt tgacccggac
    16321 ctggcgcctg gcgatttgtc ggtgatgttt ctgggcctgg ttcagcccgc ggcgattctg
    16381 tggcatgtca gcgatggcgg gtttgatgtg acacgattga cagatcgggc gtggcgggtg
    16441 tttacccaag cgatacgagg cggtttgtga tgaaaacatc aaagaagaaa aagttgatcg
    16501 ttgtgccgct gctggcagcc gtgcttgtgg gagcgtacgt ttttagtcaa cgcaacgcga
    16561 gcaatggaaa cggcgcggtg cgcatttccg ggaacatcga agcgaccgat gtcgagtgca
    16621 gcttcaaggt ggcgggctgg gtggagtccc ggctggtatc cgagggcgag acggtgcatg
    16681 cgggccagcc cgtggcgcag ctggacagcg aggacctcgc gcaggaggtg gccatgcgcc
    16741 gggcggaagt ggccgggaca gcggcgaagc tcgctgaact cgaagccggc tcgcgccagg
    16801 aagaggtcga gcgggcgcgg gctgcggtcg agctggccga ggcccgtctg gatgagctgc
    16861 tcgcgggctc gcgcagccag gaggtcaagg ctgcgcgggc atcggtacag cgggcgggcg
    16921 cggagctgac ccgggccgag accgactata accgtctgga gcagctgttc gagcggaacg
    16981 ccgtgtcggc gaaagagcgc gaccatgctt atgccgcgtt tgctaccgcg cgggcacagc
    17041 ttgctgaagc gcatgaacgg ctcggtttga cccaggaggg tccgcgccag gaacagattg
    17101 cccaggctcg ggctgcgctg tcggaggccc gggcatggtt tgagcaggtg gcgtcggggc
    17161 caaggaaaga gaccatcgaa caggcccggg ccgaattggc acgcgccgag cacgcgctcc
    17221 gcctggcgga aacgcggctg tcgtatgcgt cgatttcttc accgatatcc ggcctggtgc
    17281 tgcaggatca tgtggaggcg ggagagtatg tggcgcctgg tgcgccggtg gtgaccattg
    17341 gcgatctggg caatgtctgg ctgcgcggct acatccttga gaccgacctg ggccgggtca
    17401 aaccggggca gccggctctg atcaccacgg acacgtaccc cggcaaggcc tacgaggggg
    17461 tggtctcgtt catttcttcc gaggcggagt ttacgccgaa gaacgttcag acggccaagg
    17521 aacgcgtcaa tctggtgtac cgcatcaaga ttgatatacc gaacccggcg ctcgaattga
    17581 aaccgggcat gcccgccgac gccgagatcc atatcgacgg agcggccggg tgatgacgcc
    17641 catccgcacg gaaaacctta cgcgcacgtt tggggccgtg accgcagttg atgcgctcaa
    17701 cctggacgtt cgggaaggcg agatcttcgg gctggtgggg ccggacggcg cgggaaagac
    17761 caccacgatg cgtctgttga cggccatcat ggcgcctacg tcgggagcgg cctgggtggc
    17821 ggggcgccat accgtagacg aggcggaggc catcaaagag gagatcggct acatgagcca
    17881 gcggttcggg ttgtacccgg acctgaccgt gctggagaac attcatttct atgccgatat
    17941 ctacagcgta ccgcggcgcg gacgggcaga gcggatagat gaactcctgg ccttcagcaa
    18001 ccttaccccc ttcaaaaaac gtcaggcggg ccggctttcc ggcgggatga agcagaagct
    18061 cgggctggct tgtgcgctga tccatacgcc cagggtgctg tttctggatg agccgaccaa
    18121 cggcgtcgac cccgtgtcgc gccgcgattt ctggcggatt ctctacaagc tgctccggca
    18181 gaaagtgacc atcttcgtat cgacggccta cctcgacgaa gcggagcggt gcagccggat
    18241 tggcctgatc cacaagggcc ggttgatcgc cgtgggcacg ccggacgaag tcaaggggct
    18301 catgagcggt tcggtgctgg agctgcgctg cgcgctgccg cgaaaggcca ccgagctgct
    18361 ccggacccat ttccccccaa ataacgtggg tttgtttggc gaccgcgtgc acgtggcgac
    18421 tgaaacaccc gaggcggcgg ccgataccat tacgcgggtg ctcgctggag agggcctcgt
    18481 cgtgacgtct ttgcgcgagg tggaatccag cctggaagac gtttttattt cggtgctgag
    18541 ccatcggaaa gacgtagagg agtcctcatg aacgccggga ccgagcctgc ggttgttgtt
    18601 cgaggcctgg gacgccggtt tggtgcgttc cgcgcagtga acaacgtgac gtttgacgtg
    18661 ccccggggag agatcttcgg atttctggga cccaacggcg cgggcaagtc gaccacgatc
    18721 cgcatgctct gtggcatcct ggctccgacc gaaggcgaag ggcgggtggc gggctgtgac
    18781 ctgcgcacac aggccgagga gatcaaggcg cgcatcgggt acatgagcca gaaattctcg
    18841 ttgtatgaag acctcaccgt cgaggaaaat attgattttt acagtggcat ttaccggatc
    18901 cccgcggaga aaaagcagga acggaagacc tgggcgctcg agatggccgg gctgagcgaa
    18961 taccgcggca tgcgcacgag cctgctgtcg ggtgggtgga aacagcggct ttcactgggt
    19021 tgcgcgatgc tgcacgaacc gccggtcctt tttctcgatg agccgacgtc gggcgtggac
    19081 ccgatcagcc ggcgcgaatt ctgggaccta atttatgagc tttcaagcaa gggcgtcacc
    19141 gtgtttgtga ccacccacta tatggacgag gcggaatact gcgaccgcat tggcatgatt
    19201 taccggggtg agctggtggc gatgggcacg cccgaggctc tgaagcggga aaccatgtcg
    19261 gaagatgtcc tggaagtgct gtgcgatcgg ccaagcgacg cgatggaagt cctggaacgt
    19321 gtggaagtgg tgcaggaagc cgcgctgttc ggcaacgggt tgcacgtgac ggtggcgcgc
    19381 gcgagcggcg gcggcgcggc cgtgcaccgc gcgttggtcg aagcaggctt tgtggttcgg
    19441 cgggttgagg tcatcacgcc gtcgatggaa gacgtgtttg tgtcgctggt cgaggcacgg
    19501 gatcggctgg cggccgcgca gggcgaggtg gcggtatgaa atttattcgc gtgaaagccg
    19561 ttgcgcgcaa ggagttcatt cacatcgtgc gcgatccgcg gagcctgggc atggcgatcg
    19621 cgatccccat gctgctgctc tttttgttcg gctacgccct ggcactggac gtggaccgcg
    19681 tgcccctgat ggtgtgggac cagagccaga cgcccgccag ccgggaattt ctgacctatt
    19741 tcacggggtc gcggtacttt tcgctgcaac ggtatgcgag cggctacccg gagctcgaac
    19801 gcgccctcga cgcaaatgag gtgctggtgg gactggtcat cccgcgcgat tttgccgagc
    19861 gcgcgcggac ccgggcgccg gttcgcgtgc aggccattgt ggacgggagc gattcgaata
    19921 ccgccacgat ctcgatgggc tatctcaacg cgatttcact ggcctattcg aaccaggtgt
    19981 tgctggagga aacgcggcgg atcacgggaa aaacgcccgc acctcccgtg gatttgcgcc
    20041 cgcgggtctg gtttaacgcg gatctcgaat cccgtaatta catcataccc ggtttgaccg
    20101 ccgtgatcat gatggtgatt gccgcgctgc tcacgtcgct gaccgttgca aaagaatggg
    20161 aacaaggcac gatggagcaa ctgatctcta cgcccgtcaa agagaccgag ctggtggtgg
    20221 ggaagctgct gccctatttt gcgattggaa tgctcgacgt gctgctggcg gtgttgatgg
    20281 gcgcgtatgt gtttgaggtg ccgttgcggg ggagcgtgcc gctgctgttt ggcgtatctg
    20341 cggtgtttct ggcgggcgcc ctgtccatgg gtatgctgat cagcatcgcc gcgaagagcc
    20401 agctgctggc cagccagctc gcgatgattc tgacctttct gccgtcg
//
LOCUS       DAOPBQ010000006        20353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_2764_length_20353_cov_4.714849, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000006 DAOPBQ010000000
VERSION     DAOPBQ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 20353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20353
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2764_length_20353_cov_4.714849"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PKW60_00440"
     CDS             complement(<1..481)
                     /locus_tag="PKW60_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPJ97925.1"
                     /translation="MTDATHEAAAAPSWNPLVAFDIETTGWSPYKDALLEIAGVKFHP
                     GGPVQEMFQELIHPGRAIPPAVRKVTGITDEMVAACRPPVEVVRSFLAWAGPDACFLA
                     HNASFDIRFLNAALLNAAEPVPDLRTVDTLAWARECFPDAGSHALGDLLEILGIPMPE
                     "
     gene            complement(474..1733)
                     /locus_tag="PKW60_00445"
     CDS             complement(474..1733)
                     /locus_tag="PKW60_00445"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPJ97926.1"
                     /translation="MPEVKGFRGYRFDPNVVGSYDDAISPPYDVINPSQRMELMARGP
                     FNMAHVLLPEGEGAERYRNAAARLDSWLAQGALKQDPEPSFYLLSQTFKGLDGQELVR
                     KGFFAAARLPEYGKSSYLDHERVFDAKVADRLALTEHTRAQLGAVFVMYRDPDAQLAP
                     FYDEMRRRPEDIRAVTIDGVTQRVWKVPQDDAVTRFFRDQTLYIADGHHRFRMACLYR
                     DRMRELERPDGDRPYDFVLMGFVDIDDPGLRVWPTHRLLDPPDGFDESAFLNAAAEWF
                     DIAPADDDLAARVESAPGSAIGMAVHNGGRHILTLRDIDRKDFLGDDRTDAWRDLDVA
                     VLHRGLFERVMGLPEGAEYFYEPRAEIALAAVASGEKKLGFFLKGTRTEQICACAHAG
                     DPMPQKSTYFFPKLPSGAVIHRLVEDD"
     gene            1996..2382
                     /locus_tag="PKW60_00450"
     CDS             1996..2382
                     /locus_tag="PKW60_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97927.1"
                     /translation="MPIEFAIDRERDFTLFTGSGVLTVEDLLAVFDAYSAAGPTRLEL
                     YDFLQVTQTRLTGEDIRRISMIGVTKSSERIPGSKTVLVAASPDVYGLSRMYQLLGEV
                     EGVVWDVEVHRTLQDGYAALGMAAPD"
     gene            2422..4038
                     /locus_tag="PKW60_00455"
     CDS             2422..4038
                     /locus_tag="PKW60_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HPJ97928.1"
                     /translation="MRLTVALIVIVGAVCAAAQEGPLPLIPQPSQVQRADGHVQLTAE
                     TALVADGAPGESCDFLRMLLAPATGFSFPAAASAEGRPAIVLRIDSALEHLGSEGYRL
                     DVAPAGITLAASTPTGLLYASQTLRQLLPAQIESSEPVPGVAWTVPCVQIEDTPRFGW
                     RSCMLDSARHFQDKAFVKRFIDLLASYKMNRLHWHLADDQGWRVEIDARPRLAQVSAR
                     RPNANARLNYLPKEPSDNYGGCHSKDDLREIIAYAAARGIMIVPEIEMPGHCLAALMA
                     YPELSCTGQPEILGEQWIYPDVFCAGNDETFEFIEAVLAEVVELFPSPWIHIGGDECP
                     KTRWKACPKCQARMKAEGLATEDELQSYFVRRAEAILEKHGRRLIGWDEILEGGLAPR
                     ATVQLWRSMDGGLAAANLGHDVVMSPTSHCYLDYNYETTPVSKTYSFEPLPAELEPAK
                     IKHILGVECCMWLGQVSRRHLEATGEILPPSGIFYQVCPRMIALSEVGWSPQNTRDWA
                     DFAARLKAHGERLNLRDINYFRDPAVWPEE"
     gene            4149..7433
                     /locus_tag="PKW60_00460"
     CDS             4149..7433
                     /locus_tag="PKW60_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPJ97929.1"
                     /translation="MTIRYFLCACLVGLFALTPAIAAQTPPGRAALSLDGEWEFQLDR
                     DKNGEAAGWNAGDQAFSGRIQVPGAWDAQGFGDESDKLRHQFIGTGWYRRVVDVPAAW
                     EGQRLFLCIANVHRYAKTWVNGAYLGEHIGYLSPFEYEITGHVAPGQPAHIVIAVDSD
                     QRWEVDCLAGCIDFMDEMDAPWGGFWGHIGLEARSRAWLEDPFVQSDATGTVRVTARI
                     AGEPREGDAAQVILRDAAGTAVAELAAGAVRIERGFLEGVTRVENAKLWSTDHPHLYT
                     AELVLADASGVLDQRTVRFGMRTLEPRGSQFYLNGAPFFLRGYGDDAIYPATMIPPAD
                     KAFYLERLKVIKSYGFNGVRHHSHFLPPEYYEACDEMGILVQPELPIVYLPFYQRAQG
                     PALELYKTEWAAVITRLRNHPSVFGWCMGNELYDGIPLGEELYRIAKELDPQRMVIDS
                     DGLLRKDWLSGERRRSTLDYYAFQFDVFNLPQDTPGLYDFPGEPPRPVISHETGNFGT
                     FPLFDQIEQFEHNIKPFWFISAREKVAALGLLDEVPIWARNSQRLYTLCHKLNIEDIR
                     KKPYLSGHHWWLFQDYWTGSNGIVESYFRPKEHINPDEVRQFINDVVLLEDGLAWTCR
                     SGEALDLSFLVSNYAEAPLSDTTLACRIECAGQLLLEDIGTIPPPPQGAVTAVRDCQM
                     ICPEVSGPSLLTIRLQLTTPSRTVENAWTSWVYPQEITPALEHPVYASAEWSDELASY
                     GVTPLPEDGALPVAAAYAASALSRPLVQAARDGACLLLLKPDGVFSTVNNRFKTTWWL
                     GNARDSNVGTVVYDNPATQGMAPEGWCDGGWYRLLENASSYLLDDAPARSEVLVRGID
                     GHRFCRDRVLLFQMRLGKGSVVACGLNLEQEEVPAPEKEWLTARLLEYGASLPQPAAE
                     WPAAYFDERAMDLPPVTGPFVAGFASSTEDEVQRYVSYREEHAPYYILRQTDTANRLE
                     WTTAPVPEPFTSDHATFVFAGGLGWRTQPETPGFAFCVNGKRILNFDITDQVRQWHDS
                     DSGISLVFMPRRRLPEDTAGIFFVVVPAQHLTPGTPCTFAVESLGTGSMRWFGLNLYT
                     RVPPAK"
     gene            7691..8710
                     /gene="amrS"
                     /locus_tag="PKW60_00465"
     CDS             7691..8710
                     /gene="amrS"
                     /locus_tag="PKW60_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPJ97930.1"
                     /translation="MLEARLYEKRDGNQVKCHLCAHGCLIEDGGRGICHVRENRGGTL
                     YSLVYEQVLAENVDPIEKKPLYHVMPGSLSYSIATAGCNFRCGWCQNADISAMPRDQR
                     IIRGVRVSPDRIVQHACEAHCRSIAYTYTEPTVYFEYAYDVAERARTQGLLNVIVTNG
                     FMSAETLEAWHPLLDAANVDLKAFSDATYREHIGGRLQPVLDTLKRMKEYGIWVEVTT
                     LLIPELNDSEDELRQLTAFIARELGRETPWHISRFFPAYRMTDRRPTPPDTLERALQI
                     GEDEGLCHIYLGNIRSGRQSTRCGHCGEMIIERQGYSAIINYAKSGACPACEMPLAGV
                     AIGSG"
     gene            complement(8785..9120)
                     /locus_tag="PKW60_00470"
     CDS             complement(8785..9120)
                     /locus_tag="PKW60_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97931.1"
                     /translation="MDNSGKRMGIDMGMRCKQCNQPVTSANITESAFGAFCSDACKEK
                     YGAFVQRAHQLESMRRPRSYTRVLKSFIGKLIVILILLVALAVLGAVMPEIPVLGTVV
                     STLREKLGV"
     gene            complement(9143..11074)
                     /gene="thrS"
                     /locus_tag="PKW60_00475"
     CDS             complement(9143..11074)
                     /gene="thrS"
                     /locus_tag="PKW60_00475"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPJ97932.1"
                     /translation="MSTIRVNLPDGSHKEVEAGQNALDLAKSLGPRLAKAALGAEING
                     EVKGLATPLADGDTVAILTFDSEGGKDVFRHSASHVMASAILRVRPDAKLTIGPPIED
                     GFYYDVDAEPPLTEDDFAAIEKEMEKTVQADYPFERGEWSKDEALAYYERAGNPYKVE
                     LIRDLDDGTITYYRHDDFVDLCRGPHLPSTGKIKAFKLLSVAGAYWRGDERRPMLQRI
                     YGTAFPSQKELDEHLRLLAEAERRDHRKLGKQLDLFSFHPEGPGFPFFHPKGMIVLNQ
                     LMEFWREEHRKRHYGEVRTPIILERTLWEQSGHWDHYKENMYFTRIDERDFAVKPMNC
                     PGGMLIYKTALHSYRDLPLRLAEVGTVHRHEKSGVLHGLFRVRVFTQDDAHIFMTPDQ
                     IQDEVTDIIDFVDAVYNVFGLDYAVELSTRPEKSIGTDAMWDLATSALRNALTAKGVA
                     YKVNEGDGAFYGPKIDFHVRDCLKRSWQCATIQLDFAMPEKFDLEYVGPDGNMHRPAV
                     IHRVIYGAIERFLAVLIEHFGGAFPTWLAPVQCVVIPISDGQNAYAGQVRNRLFEAGL
                     RVEADLSDDTMKYKIRAAQTQQVPYMLVVGDRERESGAVSVRHRRKGDLGAMPLDAFV
                     SRIQEEIALKALDGAVETP"
     gene            11443..12204
                     /locus_tag="PKW60_00480"
     CDS             11443..12204
                     /locus_tag="PKW60_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97933.1"
                     /translation="MNNELLKARLNLCAVLRNLEDLPELDADAADMIRTWRLAVCFSV
                     HGGPAATLEFSEGRCRYYAACPPHPDVKLFFLSPSHLNGMFAGDANPVPLKGLSKLRF
                     LKHEFIPLTRRLEYFLRPAPETLAESAGLRAHALLTLLTGVFSVPHLAALDSIAAEIA
                     AATPCGSLQFVVDSEGPIAHLMYDGDRVEAQKGPLDRPSALMWFADWPSAYRVLSGRL
                     DAFTAAGKGLLRLDGTIPLIDNTCRIMDRAAGYLA"
     gene            12231..13469
                     /locus_tag="PKW60_00485"
     CDS             12231..13469
                     /locus_tag="PKW60_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPJ97934.1"
                     /translation="MQTFEYLQSRALFDRACEVIPGGIYGHFSPAPFGQTAAFPFFAA
                     RAEGARFQDVDGNEFIDYMCAYGLTILGYAYRAVDDAFKKQLASADAASIASPKMIEL
                     AELLVERVAGAGWAFFAKNGADVTAYAVTIARAATGRRKIVAIRGGYHGTHPWMQGPG
                     HHGVTDDELRHCIRIPWNDFAALERVVDEHGSDIAGFIASPYHHPTFQDSVLPAEGYW
                     RNVETLLRKHGIVFILDDVRCGFRLHRGGSNERYGFHPDLICFCKALANGYPIAALVG
                     TDALRTMTASVFHTGSYWFSAAPMAAAIATLQELDRLDAPHRLEAIGRKLNDGLIRIA
                     KSHGFNLRVTGEPCMAYYRITDDDSQMLHQRWCAECTRRGAYFTPHHNWFVSAAHSDA
                     ELQRTWDIADDAFRALKKTL"
     gene            13502..14335
                     /locus_tag="PKW60_00490"
     CDS             13502..14335
                     /locus_tag="PKW60_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPJ97935.1"
                     /translation="MPLTAEEKSRLKRTALEVRRQIVDVTAWSGGAHIGGALSMTDIL
                     TILYCKYLNIDPARPDWPDRDRFVLSKGHGGVGHAVMLAHRGYFPVEDLREFNATGSK
                     LGMHLDSSKAVGVDVSTGSMGHGLPQAVGLALAARVLHKSWRTYCVVGDGESNEGSIW
                     EAAMAASHYRLANLTAFCDRNRMMIDGLTEEVMGLEPFAAKWVAFGWQVRSVNGHDFD
                     ELAGAIEFALAHTGGPVMIIADTVKGKGVDFMENDPAWHYGGLGGELLAKAKASLERM
                     V"
     gene            14332..15351
                     /locus_tag="PKW60_00495"
     CDS             14332..15351
                     /locus_tag="PKW60_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004009971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPJ97936.1"
                     /translation="MSANEGGLTWTVYDADRLTQREIYGLVLTEMGERHPEIVGLSAD
                     LAKSTKIGAFGERFPERFFNFGIAEQNMFGVAAGMAKAGLVPFVSTFSSFASMRACEF
                     LRTDVCYQNLNVKVIATHGGTSFGSAGATHHSTEDLSIVRAFPNLTVIAPADGIETAR
                     AVRACMDVAGPVYIRIGRGFEPRVYDSENYPFGIGKAVEMCPGTDITVIGCGPTVYHA
                     VQAAKILREEQGLSVRVINMHTIKPIDEETILRAVTETRRIVTFEDHSVIGGLGSAVA
                     DVIAASGKGCAFEKAGIPDEFTMHGHPEDLMHLYTIDTDGILKKIGETLGRDFEADED
                     WEDEI"
     gene            15354..16133
                     /locus_tag="PKW60_00500"
     CDS             15354..16133
                     /locus_tag="PKW60_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97937.1"
                     /translation="MADDTGRITARIVLRAVLPVIKVLLEDDPKTRRRFERVTATVQF
                     AVGGAGSELMASHIIFNQGAFEIAHDLAERPDLVFRFSSYRQMNALFSGKPALPRVEG
                     VRHIGLLLKMLAVLMRLKLLMPGKMPKTPEEARLKIKLILYMVSTALSQLNRAGDPEM
                     TQWTRSQPDRVYQWSVEPEGIACYLRVKAGKTKAGRGFYIRRAPFVHMKFNGIEGALP
                     VLTNRVDTVQAITRGLVENIGSPEYGGQIGDFMLKIARMLT"
     gene            16799..17536
                     /locus_tag="PKW60_00505"
     CDS             16799..17536
                     /locus_tag="PKW60_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ97938.1"
                     /translation="MTLLEVRDLALRLNGKQVLGGVSANFKQGRVYAIVGPNGAGKST
                     LANVVMGLPGYTDCQGDILYRGESLKGLAVDDRARKGVTLAWQEPARFEGLSVHKFVT
                     AGAKTSNGRDLAAEALRQVGLEPARYLNRAVDKTLSGGERKRIELASILAIEPALVLM
                     DEPDSGIDVEALTQMFSAIHRLKEKGSTVILITHSRTVLRQAEHAYLLCDGRVIDEGP
                     AKQVGQYFENRCVLCDHTNEPGLQEVS"
     gene            17533..18468
                     /locus_tag="PKW60_00510"
     CDS             17533..18468
                     /locus_tag="PKW60_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPJ97939.1"
                     /translation="MSNAELVQRLLDSVGIKPGHVLNGDVARLEVHGNQVVGAHLVPG
                     LEVETAERDDGIEAHLRLLPGARLEKPVHLCFGMLPEKGQQHITLEIDVEEDASAQVL
                     AHCTFPNAVEVEHRMNARIRIAPGASYSYYERHVHGPGGGVQVVPHATIELGERARFK
                     TEFDLIKGRVGEMDIAYEAICQANSVLEMTARVSGSGTDTVRIHEKARLAGEAARAVL
                     ATTIALRDDARAEVFNTIIADAPHARGHVDCKEIVQGRAVAKAVPVVEVLNPSAHVTH
                     EAAIGSVDSKQLQTLLSRGLTEDEAVDLIIEGMLS"
     gene            complement(18534..19457)
                     /locus_tag="PKW60_00515"
     CDS             complement(18534..19457)
                     /locus_tag="PKW60_00515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPJ97940.1"
                     /translation="MKLSFVIPACNERESVRELAEGILHHSAGHDVQILFVDDGSTDG
                     TCEELTALRSAHPEVEVCRLRRNLGKSMALAAGFNRVSGDLVFTMDADLQDDPCEIPA
                     FLEKLEEGFDVVVGWKKVRHDPWHKVLPSRLYNAIISRVLRLRLHDINCGYKLFRREV
                     VERLSVYGERHRLLPALAYELGYRITEVPVHHNPRRYGRSKYGFERFMRGAIDVGAMM
                     FLYRFVNRPVHFFGFLSLMLLGLGGGGLVAGLARLFAAKSGLWTAAFLLGGATLCAAG
                     LLMLGLGLLWELALHFFAPVHVESYIVEEPE"
     gene            complement(19540..>20353)
                     /locus_tag="PKW60_00520"
     CDS             complement(19540..>20353)
                     /locus_tag="PKW60_00520"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HPJ97941.1"
                     /translation="VAILRNYAMLWSIERQPGALGFHYDEHCYEIYRAVKRSGHGCDF
                     VDITSDLAKYAVVFAPCACLADKALADRLDAFAQAGGTVVLTPQSGARTPANTMWAYP
                     RPGAFVAMAGLTVEEVRPYHHGQTETLEPAGGQSPVPAATVEKWVEVLACREAQPIAQ
                     FADGALKGKCAAARNARGKGAVYYLGVYLPAGALEAFAAVVLPPFPITAIPPGVEITR
                     RTGKQGSFLFVINHADQPQTLALPKAYPELLTGETLGPEVTLEANQVRVFTV"
BASE COUNT         3904 a         6597 c         6228 g         3624 t
ORIGIN      
        1 gctccggcat ggggatgcca agaatctcca acagatcgcc gagcgcatgg ctgccggcgt
       61 cggggaagca ctcacgggcc caggccagcg tgtccacggt gcgcaggtcc ggcaccggtt
      121 ccgcggcatt cagcagcgct gcattaagga agcggatatc gaacgaggca ttatgggcca
      181 ggaaacaggc atccggacct gcccaggcga gaaacgaccg caccacttca acgggagggc
      241 ggcacgccgc taccatctcg tcggtaatgc ccgtgacctt acgtactgcc gggggaatgg
      301 ccctgccggg atggatcagc tcctgaaaca tctcctgcac cggaccgccg ggatgaaatt
      361 tcactccggc gatctcgagc agggcatcct tgtacggaga ccagcccgtg gtctcgatat
      421 caaacgcgac cagagggttc caggacggtg cagcggcggc ttcatgcgtt gcgtcagtca
      481 tcctcaacca gccggtgaat gacggccccg ctcgggagtt tcgggaagaa atacgtcgat
      541 ttctgcggca tgggatcgcc ggcgtgggcg catgcgcaga tctgctcggt tcgggtgcct
      601 ttcaggaaga aaccgagctt cttctcgccc gaagcaacgg ccgcgagcgc gatctcagcc
      661 cggggctcgt aaaaatattc agcaccctcg ggcagaccca tgacccgctc gaacagtccg
      721 cgatgcagga cggccacgtc gagatcccgc caggcgtccg tacggtcatc acccaggaag
      781 tctttgcgat cgatgtcgcg cagggtcagg atgtggcgtc cgccattgtg gacggccatg
      841 ccgatggcgg aacccggagc agactcgaca cgcgcggcga gatcgtcgtc cgcgggggcg
      901 atatcgaacc attcggcggc ggcgttcagg aacgcggatt catcaaaccc atcgggagga
      961 tccaggagcc ggtgagtggg ccagactcgc aggccgggat cgtcaatgtc cacaaatccc
     1021 atcaggacga aatcgtaggg ccggtcgccg tccggccgtt cgagttcgcg catccgatcg
     1081 cgatacaggc acgccatcct gaaccggtgg tggccgtcgg cgatgtagag ggtttgatcg
     1141 cggaaaaagc gggtgaccgc atcatcctgc ggcactttcc agacccgctg ggtcacgccg
     1201 tcgatcgtga ccgcacggat atcctcaggc cgccggcgca tttcgtcata aaagggggca
     1261 agttgcgcgt ccgggtcgcg atacatcacg aacaccgcgc ccaactgggc ccgggtgtgc
     1321 tcagtcagcg ccaggcggtc ggccactttc gcgtcgaaga cccgctcatg atccaggtag
     1381 ctcgatttcc cgtattccgg cagccgggcc gccgcaaaaa agccttttcg caccagttcc
     1441 tggccatcca gccccttaaa agtctgagac aggaggtaaa acgacggttc agggtcctgt
     1501 ttcagcgcgc cctgcgccag ccacgagtca agccgcgccg ccgcgttccg gtagcgctcg
     1561 gcgccctcgc cctcgggcag cagcacgtgc gccatgttaa acgggccgcg cgccatcagt
     1621 tccatacgct gcgaagggtt gatcacgtca tacggcggcg agatcgcgtc gtcatacgac
     1681 cccaccacat tcgggtcaaa tcgatagccg cgaaaccctt tgacttccgg catggatgta
     1741 atactccctt atttgagaat gcacccggga aattcagtga aacggcggcg cccgccctgc
     1801 acgctcccct gttgacaccg gcatactaac gccgcaacct cgcgagttca agaaggagga
     1861 acggcagggg tctcatggaa cggcagcatt ttctgactgc gacgccgcgg tctgagtttt
     1921 tccttgccgc atggagatgt gctatatagt cggcaacaac gtagagcgcg gatttgagga
     1981 catgggggaa acgtcatgcc cattgagttc gcaatcgatc gcgagcgcga tttcacgctg
     2041 tttacgggta gcggcgtcct gaccgtggag gatcttctag cggtatttga cgcctattca
     2101 gcggccgggc ccacgcggct ggaactgtac gactttctcc aggtgaccca gacccgcctc
     2161 actggggaag atatccgccg tatctccatg attggagtga caaaaagctc agaacggatt
     2221 cccggcagca agaccgtgct tgtcgccgcg tcgcccgatg tgtacggact ttcgcgcatg
     2281 tatcagctgc tcggcgaagt cgagggcgtg gtctgggatg tggaagtcca ccgtaccctg
     2341 caggacggct atgccgcgct gggcatggcg gcgccggact gaggcgcggt atccgcatat
     2401 agcctgaacc aaggaggaat tatgagacta acggttgcct tgattgtcat cgtgggggcg
     2461 gtctgtgcgg ccgcgcagga aggaccgctg ccgcttatcc cgcaaccgag ccaggttcaa
     2521 cgcgcggacg gccacgtgca gcttaccgcg gaaacggccc tggttgcaga cggcgcgccc
     2581 ggcgaatcct gtgattttct gcgcatgctg ctcgctccgg caaccggatt ctcctttccc
     2641 gcagcggctt ccgcagaagg ccggcccgcg atcgtgctgc gcatcgactc cgcgctggag
     2701 cacctgggca gcgagggcta ccggctcgac gtcgcgcccg ccggcatcac gctcgccgct
     2761 tcgactccca cgggattgct ctatgcgagc cagaccctgc gccagcttct ccccgcgcag
     2821 atcgagtcga gtgagccggt accgggcgtg gcctggacgg ttccctgcgt ccagatcgaa
     2881 gacacgcccc ggttcggctg gcggagctgc atgctcgaca gtgcgcgcca cttccaggac
     2941 aaggctttcg tgaagcgttt catcgacctg ctcgccagct acaaaatgaa ccggctgcat
     3001 tggcacctgg cggacgacca gggctggcgg gtcgagattg atgcccgccc gagactggcg
     3061 caagtcagcg cacggaggcc gaacgccaac gcgcgcctga attaccttcc caaagaaccc
     3121 agcgacaact acgggggctg ccacagcaaa gacgacttgc gcgagatcat tgcctacgct
     3181 gccgcgcgcg gcatcatgat tgtcccggaa atcgagatgc cgggccactg cctcgccgcg
     3241 ctgatggcct atcccgagct gtcgtgcacg ggccagcctg aaatcctggg cgaacagtgg
     3301 atctaccccg atgtgttctg cgcaggcaat gacgagacgt ttgagttcat cgaggcggtc
     3361 ctggcggagg tcgtggagct tttcccgtcg ccgtggattc acatcggcgg cgacgagtgc
     3421 ccgaaaaccc gctggaaggc ctgcccgaaa tgccaggcac gcatgaaagc cgaaggattg
     3481 gccacggaag atgaactgca gagctatttc gtgcgccgcg ccgaggccat tctcgaaaaa
     3541 cacggccgcc ggctcattgg atgggacgaa attctcgagg gcggtctcgc gccacgggcc
     3601 acggtccagt tgtggcgcag catggatggc ggtctcgccg cggccaacct gggccacgac
     3661 gtggtcatgt cgccgacgag ccactgctac ctcgattaca actacgaaac gacgcccgtc
     3721 agcaagacgt atagcttcga accgctgccg gcagagctgg aacccgccaa aataaagcac
     3781 atattgggtg tggagtgctg catgtggctg ggccaggtgt cccggagaca tctagaagcc
     3841 accggcgaga tcctccctcc atccggcatc ttctaccagg tgtgcccgcg catgatcgcg
     3901 ctgtctgagg tgggctggtc gcctcagaat acgcgggact gggccgattt cgccgcgcgg
     3961 ctcaaggccc acggcgagcg gctcaacctc agagatataa attacttccg tgatcccgcg
     4021 gtgtggccgg aggaatgaca ggaccgtgac ccgccatgcg ggggcacgcc gcgcgagagg
     4081 tatactggcg ttttccggtg aagttgtccg gtgaaaccat agtgcgtaac ccggggaaag
     4141 gggacgttat gaccatccgt tacttcctgt gcgcttgtct cgttgggctg tttgctctga
     4201 caccagcaat cgccgcgcag accccgcccg ggcgcgcagc gctctcgctc gacggagagt
     4261 gggagtttca gctcgatcgc gacaaaaatg gtgaggcggc cggctggaac gccggagatc
     4321 aggccttcag cggccgcatc caggtgccgg gcgcgtggga cgcccaggga ttcggcgacg
     4381 agagcgacaa actgcgccac cagttcattg gcacgggctg gtaccgcagg gtggtcgacg
     4441 tgcccgccgc ctgggaaggg cagcggctgt ttctctgcat cgccaacgtg caccgctacg
     4501 cgaagacctg ggtgaacggc gcgtacctgg gcgagcacat cgggtatctg tctcccttcg
     4561 agtacgagat caccgggcac gtggcccccg gacaacccgc gcacatcgtg attgcggtcg
     4621 attcggacca gcgctgggag gtggactgcc tcgccggatg catcgatttc atggacgaga
     4681 tggacgcgcc ctggggcggg ttctggggac acatcggcct ggaagcgcgc tcgcgggcct
     4741 ggctggagga ccccttcgta caatccgacg ccacgggcac cgtgcgggtc acggcccgca
     4801 ttgccgggga gccgcgggaa ggcgacgcgg ctcaggtgat tttgcgcgat gccgccggca
     4861 cggccgtcgc tgagctggca gccggggcgg tccgcatcga gcggggattt ctggaaggtg
     4921 tcacgcgggt tgagaacgcg aagctctggt ccaccgatca tccgcatctg tacacggcag
     4981 aactcgtgct ggcagacgct tccggcgtac tggaccagcg cacggtgcgc ttcggcatgc
     5041 gcacgctgga gccccgcgga tcacagttct atctcaacgg cgcgcccttc tttctgcggg
     5101 gatacggaga cgatgcgatc tacccggcca cgatgatccc ccccgcggac aaggcgttct
     5161 atctggagcg cctgaaggtg atcaaatcgt acggattcaa cggcgtgcgg catcacagcc
     5221 attttctgcc gccggaatac tatgaagcct gcgacgaaat gggcattctc gtgcaacccg
     5281 aacttcccat cgtgtacctg ccgttctatc agcgcgccca gggccccgcg cttgaactct
     5341 acaaaaccga atgggccgcg gtcatcacgc ggctacgcaa ccacccctcg gtatttggat
     5401 ggtgcatggg caacgaattg tacgacggca tccccctggg cgaagagctg taccgcatcg
     5461 ccaaggaact ggacccgcaa cgcatggtca tcgactccga cggccttctc cgcaaagact
     5521 ggctgtcggg agaacgccgg cgttccacgc tcgactacta tgcgttccag tttgacgtct
     5581 tcaacctccc gcaggacacc cccgggctct acgacttccc cggcgagccg ccccggcccg
     5641 tgatctcgca tgaaaccggc aatttcggca cgtttcctct gttcgaccag atagaacagt
     5701 tcgaacacaa catcaaaccg ttctggttca tctcggcgcg ggaaaaagtc gcggcactcg
     5761 ggctcctcga cgaggtcccg atctgggcgc gcaattccca gcgcctctat acgctctgcc
     5821 acaagctcaa catcgaagac atccgcaaaa agccctatct ctcagggcac cactggtggc
     5881 tgttccagga ttactggacc gggtcgaacg ggatcgtcga gtcctatttc cgcccaaagg
     5941 aacacatcaa cccggacgag gtgcgccagt tcatcaatga cgtggtcctg ctcgaagacg
     6001 ggctggcctg gacctgccgg tcaggcgagg ccctggatct ctccttcctc gtgtcgaact
     6061 atgcggaggc tccgctttcc gatacaacgc tcgcctgccg catcgaatgc gctggacagc
     6121 tcctcctgga agacataggg accattccgc ctcctcccca gggcgcggtg acggccgtcc
     6181 gcgattgcca aatgatctgt cccgaggttt cagggcccag cctgctcacg atcagactgc
     6241 agctcaccac cccgtcgcga actgtcgaga acgcctggac ctcgtgggta tatccgcagg
     6301 agatcacgcc cgctctcgaa catccggtat atgccagcgc cgagtggagc gacgaactcg
     6361 cctcatacgg cgtcaccccg ctgcctgaag acggcgcgct ccccgtggcg gccgcatatg
     6421 ccgcttcggc cctgtcgagg cccctggttc aggcggcccg cgacggcgcc tgcctgctgc
     6481 tcctcaaacc ggacggcgtg ttctccaccg tgaacaaccg gttcaagacc acgtggtggc
     6541 tcggaaatgc gcgcgacagc aacgtcggga cggtggtcta cgacaatcca gccacgcagg
     6601 gcatggcgcc cgaaggctgg tgcgacggcg gctggtaccg gcttctcgaa aacgcctcct
     6661 cctacctcct cgacgacgcc ccggcgcggt ccgaggtcct ggtgcgcggt atcgacggcc
     6721 accggttctg ccgtgatcga gtgctcctct tccagatgcg cctgggcaaa ggatccgtgg
     6781 tcgcctgcgg cctgaatctc gagcaggagg aggtcccggc ccctgaaaaa gagtggttga
     6841 ccgcgcggct cctggaatac ggggcctcgc tgccgcagcc ggccgcggaa tggcccgcag
     6901 cctatttcga cgaacgggcc atggatctcc cgcccgtgac cgggccgttc gtcgcgggat
     6961 tcgcctcatc gacggaggat gaggtccaga ggtacgtgag ttaccgcgag gaacacgcgc
     7021 cctactacat ccttcgccag accgatacgg ccaaccgcct cgaatggacc accgcgcctg
     7081 tgcccgaacc gttcaccagc gatcacgcca ccttcgtgtt cgcgggcggg ctcggctggc
     7141 gcacccagcc cgaaaccccg ggctttgcct tctgcgtcaa cggaaaacgc atcctcaact
     7201 tcgatatcac cgaccaggtg cggcaatggc atgacagcga ttcaggcatc tctctggtgt
     7261 tcatgccccg gcggcggctc cccgaggaca cggccggcat tttcttcgtg gtggtgcccg
     7321 cgcagcacct cacaccgggc acgccctgca ccttcgccgt cgagtccctg ggcacgggca
     7381 gcatgcgctg gttcggcctc aacctctaca cccgcgtccc tcctgcaaaa tagccggcat
     7441 gggaggagga taggaacata cagcgatgta tgggatggca gacggcccga tgaggctgtt
     7501 ttagagcggt ttaatggtac atacggcggc ggcgcaggag ccgcgcccga ccgggttgcc
     7561 tggcgtaacg gcggactgga ctgggcgcgc gccgggttac atgcgggcac gcactttcgt
     7621 atactgacga cggcgcagcg aaatgcaacg gcggcacgcg gacaatggtc caccgccgga
     7681 aaggacgccc atgctcgaag ccaggcttta cgaaaaacgc gacggcaacc aggtgaaatg
     7741 ccatctctgc gcgcatggct gcctcattga agatggcggg cgcggaatct gccacgtgcg
     7801 ggaaaaccgc ggcggcacgc tgtattcgct ggtctacgag caggtcctgg ccgaaaacgt
     7861 cgatccgatc gaaaaaaaac cgctctatca cgtcatgccc ggctcgctgt cgtattccat
     7921 cgcaacggcc ggctgcaatt tccgctgcgg ctggtgccag aacgccgaca tctcggccat
     7981 gccgcgcgac cagcgcatca tccgcggcgt ccgggtctcg cccgaccgga ttgttcagca
     8041 cgcgtgtgag gcccactgcc gcagcatcgc ctatacctac acggagccca cggtctactt
     8101 cgagtatgcc tatgacgttg cggaacgcgc ccgaacacag ggcctgctca acgtgatcgt
     8161 cacgaacggg tttatgagcg cggagacgct cgaagcgtgg catccgctgc tggacgcggc
     8221 caacgttgac ctgaaggcgt tcagcgacgc gacctaccgg gaacacatcg gggggcgtct
     8281 ccagcccgtg ctcgatacgc tgaagcgcat gaaagagtac ggcatctggg tggaagtcac
     8341 cacgctgctc atcccggaac tgaacgacag cgaggacgaa ttgcgccagc tcacggcgtt
     8401 catcgcccgg gaactcgggc gtgaaacccc gtggcatatc agccgctttt tccccgccta
     8461 caggatgacg gatcggcggc cgacgccgcc cgacaccctg gaacgcgccc tgcagatcgg
     8521 cgaagacgag gggctctgcc acatctacct gggcaatatc cgcagtggcc gccagagcac
     8581 ccgctgtggc cactgtggcg agatgatcat cgaacgtcag ggatacagcg ccatcatcaa
     8641 ctacgcgaaa tcgggcgcct gccccgcatg tgaaatgccc ctggccggtg tcgccatcgg
     8701 ttccggttga cggggctgtc cacggactcc acccgccccg gcttcacacc cagcacccct
     8761 cccacaatgc ggcggaacgc cgggctagac cccgagtttc tcgcggagcg tgctgaccac
     8821 agtgccgagg acgggaatct cgggcatgac cgcgccaaga accgccagcg ccacgagcag
     8881 gatcagaatc acaatcagct tgccgatgaa cgatttgagc acgcgcgtgt acgaccgggg
     8941 tctccgcatg ctctcaagct ggtgcgcgcg ctgcacgaaa gctccgtatt tttctttgca
     9001 cgcgtcgctg caaaatgccc caaatgccga ttcggtgatg ttggcgctgg tgaccggctg
     9061 attgcactgt ttgcagcgca tccccatatc gatgcccatg cgcttgccgg aattgtccat
     9121 ctccctgtcc tcccgggcag cgttacggcg tctcgacggc gccatccagt gctttgagcg
     9181 caatttcctc ctgaatgcgc gatacaaacg catccagggg catggcgccc aggtcgcctt
     9241 tccggcgatg ccggaccgag accgcccccg attcacgttc ccggtcaccc acgacgagca
     9301 tgtagggtac ctgttgcgtc tgggcggccc ggatcttgta cttcatcgta tcatcgctca
     9361 ggtcggcttc cacccgcaag cctgcctcaa agaggcggtt gcgaacctgg ccggcatagg
     9421 cgttctgacc gtcggaaatg gggataacga cgcattgaac cggcgccagc caggtcggaa
     9481 acgcgccgcc gaaatgttcg atcagcacgg ccagaaaccg ttcgatcgcg ccgtaaatca
     9541 cccggtgaat gaccgcggga cggtgcatgt tgccgtccgg cccgacgtat tcgaggtcaa
     9601 acttctcggg catggcgaaa tcgagctgga tggtcgcgca ctgccagctc cgtttcaggc
     9661 agtcgcgcac gtgaaagtcg atcttggggc catagaacgc gccgtcgccc tcgttcactt
     9721 tatatgcgac gcccttggcg gtaagcgcat tgcgcagcgc gctggtggcg aggtcccaca
     9781 tcgcgtccgt gccgatggat ttttccggcc gggtgctcag ctcgacggca taatccaaac
     9841 cgaagacgtt gtagacggcg tccacaaagt cgatgatatc ggtcacctcg tcctggatct
     9901 ggtcgggcgt catgaagata tgggcgtcgt cctgcgtgaa cacgcgcacg cgaaacaggc
     9961 cgtgcaagac ccccgatttc tcatgccggt gaaccgttcc cacttcggcg agccgcagcg
    10021 ggaggtcgcg gtagctgtgg agcgcggtct tgtagatcaa catgccgccg gggcaattca
    10081 tcggtttgac ggcgaagtcc cgctcgtcga tccgggtgaa atacatgttt tctttgtagt
    10141 ggtcccagtg accggactgc tcccacagcg tgcgttcgag gatgatcgga gtgcgaacct
    10201 cgccgtaatg ccgtttgcgg tgctcctcgc gccagaattc catcagctgg ttcaagacga
    10261 tcatgccctt ggggtggaag aacggaaatc cgggcccttc gggatgaaaa ctgaacagat
    10321 cgagctgttt gcccagtttt cggtggtcgc gccgttcggc ttccgccagc agccgcaagt
    10381 gctcgtcgag ttccttctgg ctcgggaagg ccgttccgta gatgcgctgc agcatggggc
    10441 gccgctcgtc accccgccag taggccccgg ccacactcag cagcttgaac gccttgattt
    10501 ttcccgtcga cggcagatgc ggtccgcggc agaggtccac gaaatcatcg tgccggtaat
    10561 aggtgatcgt gccgtcatcg agatcgcgga tcagctcgac tttgtaggga tttcccgccc
    10621 gttcgtagta cgccagggcc tcgtccttgc tccattcacc ccgctcgaac gggtagtccg
    10681 cctgaacggt cttttccatc tctttttcga tggcggcaaa gtcgtcctcg gtcaggggcg
    10741 gctcggcgtc aacgtcgtaa tagaatccat cctcgatagg cgggccgatg gtgagcttcg
    10801 catcgggccg cacccggaga atggccgagg ccatcacgtg cgaggcactg tgccggaaca
    10861 catctttgcc cccttcgctg tcaaacgtga ggatcgctac cgtgtcgcca tcggccagcg
    10921 gagtggccaa tcccttgacc tcgccgttga tttcggcgcc cagcgcggct ttggcaagcc
    10981 gcgggccgag gctctttgcc aggtcgaggg cgttctggcc tgcttccact tctttatgcg
    11041 acccgtccgg caggttcacg cgtattgtgc tcatgacaac tcctgaattc cgatgcgtta
    11101 cgccgctgat atgttgccgc gccctgtaga aaaacgcagg ggtatgcaca ccggtttcgc
    11161 ctgatcccgc gtgcagaggc gccgacaata tcccgaaatt gtgccatgaa ccgcaccgta
    11221 aggtaaagcg aaggccctgc gcctgtcacc gcaacgtgca aatgccatcc cgtgcaaagt
    11281 agaaatgtcc tcgtatccca cgcccagccc ccggaaaacc ggccctgcgg ggaccgattg
    11341 cgctcatacc tcacctgtga tagcatcgtg tacggtactg gatgggggtc gcgtacgtgt
    11401 agagctggga taaccggtgg ctttctgcgc gagatatact ctttgaacaa tgagctgctg
    11461 aaagcgcgtc tcaacctgtg tgccgtcctg cgtaacctcg aagatctgcc cgaactcgac
    11521 gcggacgccg cggacatgat ccgcacctgg cgcctggccg tatgtttctc cgtgcacggc
    11581 gggcccgccg cgaccctcga attcagcgaa gggcgttgcc ggtattatgc cgcgtgcccg
    11641 ccccaccccg acgtaaagct ctttttcctg tcgccgtctc acctgaacgg gatgtttgcc
    11701 ggagacgcga acccggtacc gctcaaaggg ctctcgaaac tgcggttttt gaaacacgaa
    11761 ttcatcccat tgacccggcg gctggaatat ttcctccgcc cggccccgga aaccctggcc
    11821 gaatccgccg gattgcgcgc tcatgcgctg ttgacgctcc tgacgggcgt gttttccgtc
    11881 ccgcacctgg cggcgctcga ctccatcgcg gcggagatcg ctgccgcaac cccctgcggt
    11941 tcgctgcaat tcgtcgtgga cagcgaaggc ccgatcgctc atctcatgta tgacggcgac
    12001 cgcgtggagg cgcagaaagg cccgcttgac cggccgtcgg ccctgatgtg gttcgccgac
    12061 tggccgtcgg cctaccgcgt tctcagcggc cggctcgacg cgttcacggc tgcgggaaaa
    12121 ggattgttgc ggctggacgg caccattccg ctcattgaca atacatgccg catcatggat
    12181 cgcgctgccg gctatctggc ctgagccggg atgaggaggg gacggagcac atgcagacgt
    12241 tcgaatacct gcaatcccgc gcgcttttcg accgcgcctg tgaagtcatc cccggcggca
    12301 tctacggcca tttcagtccc gcgcccttcg gccagaccgc cgcgtttcca ttctttgccg
    12361 cccgcgccga aggcgcccgg ttccaggatg tcgacggcaa cgaattcatc gactacatgt
    12421 gcgcgtatgg cctcacaatc ctcggctatg cctaccgcgc cgtcgacgac gccttcaaaa
    12481 aacagctcgc aagcgcggac gccgccagca tcgcatcgcc gaaaatgatt gagctggccg
    12541 agctgctcgt ggagcgcgtg gccggcgccg gctgggcctt tttcgcgaag aacggcgccg
    12601 atgtcacggc ctacgcggtt accatcgccc gcgccgccac cggacgccgc aagatcgtcg
    12661 cgattcgcgg cggctaccac ggcacgcatc cctggatgca gggccccggc caccacggag
    12721 tcaccgacga cgaactccgt cactgcatcc gcatcccctg gaacgacttc gcggcgctcg
    12781 aacgggtggt ggacgagcat ggaagcgaca tcgccggatt catcgcgagc ccgtaccatc
    12841 atcccacgtt tcaggacagc gtactccccg cggagggata ttggcgaaac gttgaaaccc
    12901 tccttcgcaa acacggcatc gtgttcatac tcgacgacgt gcgctgcgga ttccgcctcc
    12961 accgcggcgg cagcaacgag cgctacggtt ttcatcccga tctcatctgt ttctgcaaag
    13021 cgctggccaa cggctacccc atcgccgccc tggtcggcac ggacgccttg cgcaccatga
    13081 ccgccagcgt atttcacacc ggcagttact ggttctccgc cgcacccatg gccgcggcca
    13141 tcgcaacgct ccaggaactc gaccgcctcg acgcgccaca ccggctcgaa gccatcggcc
    13201 ggaaactcaa cgacggcctc atccgcatcg caaagagcca cgggtttaac ctccgggtca
    13261 ccggtgaacc ctgcatggcg tattaccgca tcaccgatga tgactcgcag atgcttcatc
    13321 agcgctggtg cgccgaatgc acccggcgcg gcgcctattt cacgccgcat cacaactggt
    13381 tcgtctcggc tgcccacagc gacgccgaac tccagcgcac gtgggacatc gccgacgacg
    13441 cgttccgcgc actgaaaaag accctgtgaa gaaaccgagg tgacagcccg ggaggtcagg
    13501 catgccactc actgccgaag agaaaagccg cctcaagcgc accgctctcg aggtccggcg
    13561 gcagatcgtc gatgtcaccg catggtcagg cggcgcgcat atcggcggcg ccctgagcat
    13621 gaccgacatc ctcaccatcc tgtactgcaa atacctcaac atcgacccgg cccggcccga
    13681 ttggccggac cgcgaccggt tcgtgctcag caaagggcac ggcggcgtag ggcatgccgt
    13741 gatgctggcc caccggggct actttccggt ggaggatctc agagaattca acgcaaccgg
    13801 ctctaaactc ggcatgcacc tcgattcaag caaggccgtg ggtgttgatg tctcgaccgg
    13861 ctcgatgggc cacgggttgc cgcaggccgt ggggctcgcg ctcgccgccc gcgtgcttca
    13921 caagagctgg cggacctatt gcgtcgtggg tgatggggaa agcaacgagg gctccatctg
    13981 ggaagccgcc atggcggcca gtcactacag gcttgcgaac ctcacggcgt tttgcgaccg
    14041 caaccgcatg atgatcgatg gtctcaccga agaagtgatg gggctggaac cctttgccgc
    14101 gaaatgggtc gcgttcggct ggcaggtacg cagcgtcaac ggccacgatt tcgatgaact
    14161 ggccggcgct atcgagttcg cgctcgcgca caccggcgga cccgtcatga tcatcgcgga
    14221 tacggtcaaa ggcaaaggcg tggatttcat ggagaacgac ccggcctggc attacggcgg
    14281 actgggcggc gaactgctgg cgaaagcgaa ggcatctctg gagcgcatgg tatgagcgca
    14341 aacgaaggcg gactcacctg gaccgtgtac gacgcggaca gactcaccca gcgcgagatc
    14401 tacggcctgg tgctaaccga gatgggcgaa cgccaccctg agatcgtcgg gctcagcgcc
    14461 gatctcgcca aatctaccaa gatcggcgcc tttggcgaac gcttcccgga gcgtttcttt
    14521 aatttcggca tcgccgaaca gaacatgttc ggcgtggccg cgggcatggc caaagcgggc
    14581 ctcgtcccgt tcgtctcgac gttctcctct tttgcctcca tgcgggcctg cgaattcctc
    14641 cgcaccgatg tctgttatca gaacctcaac gtcaaagtga tcgccactca tggcggcacc
    14701 tcgttcggtt ccgcgggcgc cacccaccat tctaccgagg atctgtccat cgtccgcgca
    14761 ttcccaaacc tcacggtgat cgcccccgcc gacggcatcg aaaccgcccg ggccgttcgc
    14821 gcctgcatgg acgtcgccgg cccggtgtat atccgcatcg ggcgcggctt cgaaccgcgc
    14881 gtttacgaca gcgaaaatta ccccttcgga atcggcaaag cggtcgaaat gtgcccgggc
    14941 acggacatca ccgtcatcgg ttgcgggccg acggtatacc acgcggtcca ggccgcgaaa
    15001 attctccgcg aagaacaggg tttgagtgtc cgtgtgatca acatgcatac catcaagccc
    15061 atcgacgagg agacgatcct gcgcgccgtg accgagacgc gccgcatcgt gaccttcgaa
    15121 gaccacagcg tcattggagg gctgggcagc gccgtcgccg atgtcatcgc cgccagtgga
    15181 aaaggctgcg ccttcgaaaa agccgggatc cccgacgaat tcacgatgca cggccatcct
    15241 gaagatctca tgcacctcta cacgatcgac accgacggca tcctcaaaaa aatcggcgag
    15301 accctgggac gcgatttcga ggctgacgaa gactgggaag acgagatcta gccatggcgg
    15361 acgacaccgg ccgcatcacc gcacgcattg tactacgggc cgtgctgccc gtcatcaagg
    15421 tcctgctcga agacgatcca aaaacccgga gacgcttcga gcgcgtcacc gccaccgtac
    15481 agttcgcagt cggcggcgct ggcagcgagc tgatggcatc gcatatcatc ttcaaccagg
    15541 gcgcttttga aatcgcccat gatttggccg agcgccccga cctcgttttc cggttttcgt
    15601 cgtaccgcca gatgaacgcc ctgttctccg gaaaaccggc cctaccgaga gttgaaggcg
    15661 tgcggcatat cgggttgttg cttaaaatgc ttgccgtgct gatgcgtttg aaactgctga
    15721 tgcccggcaa aatgcccaaa actcccgaag aagcccggct caaaatcaag ctcatcctct
    15781 acatggtttc gaccgccctg agccagttaa accgggccgg cgatcccgag atgacccaat
    15841 ggacccgttc acagcccgac cgcgtctatc agtggtccgt tgagccggaa ggcatcgcgt
    15901 gctatctgcg cgtaaaagcc ggcaaaacca aggccggccg cggcttctat atccgccggg
    15961 cgccgtttgt ccacatgaaa ttcaacggca tcgaaggagc cctgcctgta ctcaccaacc
    16021 gcgtcgacac cgtacaggca ataacccgtg gactcgtcga aaacatcgga tcacccgaat
    16081 acggcggcca gatcggcgat ttcatgctca aaatcgcccg catgctgacc tgatcagcct
    16141 gtgcgcccac accggatgcc tgtagtgcgg tcactggaag ccaggggctg taatccgcct
    16201 caaaactcca cccccaaatc ggctatcgcc gattcacccc cgccagcggg ggacaggcgc
    16261 gccgtaccga aatctccggg ggtccgggtt ctgatgtctc ccgttgcgag gctggcggac
    16321 gtggcacggt tgccctccgg attgcagggg cagccacagg gggggccgcc catcccagcc
    16381 ctacgcgttg actcgaatgc agagcaggca cgggggcctg cccctacaca cacgcttcgc
    16441 gtggccggag gcggagaata gccgacagcc caagggtatg aaaacatgga ccgcgtggac
    16501 cgtgtggact cccgtcttaa cttctccaag ctcgaatcga acagctctcg ctgcacggct
    16561 tcgctgtctt gtgtcccccg ctggcggggg tggacacgcg tctcgcgtgg ccgggggtgg
    16621 atttatcgcg gacatcattc tctcttccat gctttggtac ggtcgcaaag cgcgcgacga
    16681 cgccatcccg ctgaaatttt cagccggcgc gattcggcag ctttatgatg gaccctcaaa
    16741 cggggtacaa taaccgggtt tcttcaaatc gcaccccggg tgcgggaaag gatctcagat
    16801 gacccttctg gaagtgcggg acctcgccct gcggctcaac ggcaagcaag ttctgggcgg
    16861 cgtatccgcc aatttcaagc agggccgggt gtatgcgatc gtcgggccga acggcgcggg
    16921 caaatccacc ctggccaacg tggtcatggg tctgccggga tatacggact gccagggcga
    16981 catcctgtac cggggtgaat cgttgaaagg cctggcggtt gacgaccggg cgcgtaaagg
    17041 cgtgacgctg gcctggcagg aacccgcgcg atttgaaggc ctgtccgtgc acaaattcgt
    17101 gacggcgggc gcgaaaacgt ccaacggccg cgacctcgcc gccgaagcgt tgcgccaggt
    17161 cggactggaa cccgcccgct acctgaaccg cgccgtcgac aagacactga gcggcggcga
    17221 gcgcaagcgc atcgagctgg cgtcgatcct cgccatcgaa cccgcgctgg tgctcatgga
    17281 tgagcccgac tccggcatcg atgtggaagc cctcacccag atgttcagcg ccattcaccg
    17341 ccttaaggaa aagggcagca cggtgatcct gatcacgcac agccgcaccg tgttgcgtca
    17401 ggccgagcat gcctacctgc tttgcgatgg gcgtgtcatt gacgaaggcc ccgccaaaca
    17461 ggtcggccag tactttgaaa accgctgtgt cctgtgcgac cacaccaacg aacccggcct
    17521 gcaggaggta tcatgagcaa cgccgaactc gtccagcgcc tgctggattc agtgggcatc
    17581 aagccgggcc atgtgctgaa cggcgatgtc gcccgccttg aggtccatgg aaatcaagtg
    17641 gtcggcgcgc acctggtgcc cggcctcgaa gtcgagacag ccgagcggga tgacggcatc
    17701 gaggcgcatc tgcggctgct gccgggagcc cggctcgaaa agccggtcca cttgtgtttc
    17761 ggcatgttgc ccgaaaaggg ccagcagcac attaccctgg aaatcgacgt ggaagaagac
    17821 gcttcagcgc aggttctggc ccactgcacc ttccctaatg cggttgaggt ggaacaccgg
    17881 atgaacgcgc ggatccgcat cgccccgggc gccagctatt cctactacga gcggcatgtc
    17941 cacggccccg gcggcggcgt gcaggtggtc ccgcacgcaa ccatcgagct cggcgaaaga
    18001 gcgcggttca agaccgaatt cgatctcatc aaaggccgtg tcggcgagat ggatattgcg
    18061 tacgaggcga tctgccaggc gaacagcgtg cttgagatga cggcccgcgt gagcggcagc
    18121 ggcaccgaca cggtgcgtat ccacgaaaaa gcccgcctgg ccggtgaagc ggcccgcgcc
    18181 gtgctcgcaa ccacgattgc cttgcgtgac gatgcccgcg ccgaagtgtt caataccatc
    18241 atcgcggatg cgccccacgc ccgcggtcat gtcgactgca aggagatcgt ccagggccgc
    18301 gcggtggcca aggcggtccc ggtcgtggaa gtgctcaacc ccagtgccca tgtcacccac
    18361 gaagccgcca tcggcagcgt agactccaaa cagctgcaga ctctgttatc gcgcgggctc
    18421 acggaagacg aagcggtcga tctcatcatc gaaggcatgc tgagctgaga tcggcacgcc
    18481 gggcgctgtg tctgtcgcgg ctacttcggg gtgccgggtg ctgcgggcgg attctattcc
    18541 ggctcctcga caatgtagct ttccacatgg accggagcga agaaatgcag cgccagctcc
    18601 cacagcagcc cgagccccag catcagcagg ccggcggcgc acagcgtggc gccgccgagc
    18661 aggaacgcag cggtccacaa tccggatttc gcggcgaaaa gccgcgccag gcccgcaacc
    18721 aggccgccgc cgccgagccc cagcagcatc aggctcagga aaccaaaaaa gtggaccgga
    18781 cgattcacga accggtacag aaacatcatg gcgccaacgt cgatggcccc gcgcatgaac
    18841 cgttcgaaac cgtattttga acggccgtaa cgccgcgggt tgtggtgcac gggcacctcg
    18901 gtaatgcggt atcccagttc gtaagccagt gccggcaaca agcggtgccg ctcgccgtag
    18961 acagagagcc gctcgaccac ctcccgccga aacagcttgt agccgcagtt gatgtcatgc
    19021 agtctgagcc tgagcacccg cgagatgatc gcgttgtaca ggcgggacgg cagtacctta
    19081 tgccagggat cgtgtcggac ttttttccac cccacgacca catcgaagcc ttcttccagc
    19141 ttttcgagaa aggccgggat ctcgcatgga tcatcctgca gatcggcatc catggtaaac
    19201 accaggtcgc ccgatacccg gttgaatccc gccgcaagcg ccatcgattt gccgagattg
    19261 cgccgaagcc ggcagacctc gacttcgggg tgggcggaac ggagagccgt cagttcttca
    19321 caggtgccgt cggtgctgcc gtcgtcaacg aacagaatct gcacgtcgtg tccggcgctg
    19381 tggtgcagaa ttccttcggc cagttcccgc accgattcgc gctcgttgca ggcggggatg
    19441 acaaaactca acttcatgca ggctgcgccc tccttccctc gaaggattgt atcacggata
    19501 gcccgcgcag cgggccggcg ttgagaggcg cgggcccggt cagaccgtga agacccgcac
    19561 ctggttcgcc tcgagtgtga cttcaggacc cagggtctcg ccggtgagca actccggata
    19621 cgccttcgga agtgcgaggg tctgcggctg atcagcgtgg ttgatgacaa aaaggaacga
    19681 cccctgcttg ccggtacggc gggtgatctc cacgcccggc ggaatcgcgg tgatcgggaa
    19741 gggcggaagc acgaccgcgg caaacgcttc cagggcgccg gccggaagat atacgccgag
    19801 gtaatagacg gcgcctttac cccgggcatt gcgcgccgcc gcgcacttgc ccttcagcgc
    19861 gccgtccgca aactgcgcaa tgggctgcgc ttcgcggcag gcgagcactt caacccattt
    19921 ctccacggtg gccgcgggca cgggcgactg gccgcccgcg ggctcgagtg tttcggtctg
    19981 gccgtgatga tatgggcgga cctcctcgac cgtgagcccc gccatggcaa caaatgcgcc
    20041 ggggcgcggg taggcccaca tcgtgttcgc aggcgtgcgc gcacccgatt gcggcgtcag
    20101 cacgaccgtg ccgcccgcct gggcgaacgc gtcgagccgg tcagcgaggg ccttgtcggc
    20161 caggcatgcg cacggggcaa acaccacggc gtattttgcg agatcgctgg tgatgtcgac
    20221 gaaatcgcag ccgtggccgc tgcgtttgac cgcgcggtag atctcgtagc agtgctcgtc
    20281 gtaatggaag cccagggcgc ccggctgccg ttcgatgctc cacagcatcg cgtagttgcg
    20341 caagatcgcg acc
//
LOCUS       DAOPBQ010000007        19111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_2976_length_19111_cov_4.159267, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000007 DAOPBQ010000000
VERSION     DAOPBQ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 19111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19111
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2976_length_19111_cov_4.159267"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PKW60_00525"
     CDS             complement(<1..526)
                     /locus_tag="PKW60_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPJ97942.1"
                     /translation="MTGKMASWGTYFRRASLAVVIAGSVWLLWPRAGGEFASGAPEHR
                     NNGAEPRFVIKFVPGQQYMPGTTPFGLGEPLRGIADVVRDFEARFPDTRVELITVPGV
                     REYLVTQLSSGSAPDILMVNVEDVWVDVQKGWYVPLDSYLEAPNPFIREKGDPAQPGY
                     GQWWDMFKYQAISRG"
     gene            complement(721..1182)
                     /locus_tag="PKW60_00530"
     CDS             complement(721..1182)
                     /locus_tag="PKW60_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPJ97943.1"
                     /translation="MLEMDILGVSLSEEHHPPVVLLRHGDRVLPIVVGRAEAQAIHTG
                     MIHQDLGRPMTHDLICNVLAGLRGELKSVTIYKLENETFYAHLNVEQQSVDGQVEQVL
                     RIDSRPSDGIAIATRVGCPIFAAEEVLEIAGQDVANLGPGDDEDEEDDEFE"
     gene            complement(1510..2367)
                     /locus_tag="PKW60_00535"
     CDS             complement(1510..2367)
                     /locus_tag="PKW60_00535"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HPJ97944.1"
                     /translation="MMMRTALAGMIAVLGCQAALGAEQPVPKNVLFLLTSEGFAHSPT
                     VAKDGEPPLAEKILRPILEKMGASLTVTYDASTINAENLKNYDLVMFYTQGDLTRPNM
                     TNTPPMGPNGQTELIQWIEKGGRFLGFHSATDTFDVEGDVITPYISMIGAEFKTHGQQ
                     FVGKVQVVDPQHPAMARIPDGWELNEEWYLFHKFNVDAIHVLALMDPGAERAKQEMYN
                     IAPYPVIWIRALGQGRVYFTALGHREDLWEGQQFQQAFVDAVHWLMGTGDPQAEPNYR
                     KVVPDAAAK"
     gene            complement(2475..3329)
                     /locus_tag="PKW60_00540"
     CDS             complement(2475..3329)
                     /locus_tag="PKW60_00540"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPJ97945.1"
                     /translation="MRGRVEAIDDIATLPEVLTRIIAVVQDESTTALDLADEIVRDQA
                     LAMRVLRVVNSSFYGFPRAISNIPEAVVILGFNEIERLALTISVINIFGKSPAHVRAL
                     HNLWRHSLACSLAAGTLEQHFRRLYPEIAGAHLAGLLHDIGKAVIIQYFPDELTRVIH
                     MVESLGCSAYEAEYEVFGGITHGEIGAWLAERWQLPEVLVQSIAGTHRPSDVPGNPIM
                     PHATHLADALVNMMGIGALNDQPQALSPNPESLERLPLDEGLVTRIQERLNKQRGLLG
                     AVAVGALS"
     gene            3682..5436
                     /locus_tag="PKW60_00545"
     CDS             3682..5436
                     /locus_tag="PKW60_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPJ97946.1"
                     /translation="MKPCETIRVLTGLRKGETCPITGTLTIGRSPHSGLQLNELEVSR
                     RHAVIQQTALGTLIRDLGSGNGTYVHNHRIVEYRLSHGDVISIGPISLRFESCAAELP
                     SHSGSPSVVFEEGGVENVQALTAENVHRTFLAAPLSAQTAEQARKAQERLAAVYAANQ
                     VISSERDLNRLFERVLDQIFGLVPAHNGAILLADPATSRLEPAHMRKGRDENEHVTIS
                     STIVQRAYDQSEAILVFDAADDSRLGHGQSIILHNIASAMCTPLQVHDETLGVLYVDT
                     RGTTNAFTEGDLELLVALAGPAAIAIKNAQYVDKLESAYQGTLVMTANAIEARDHYTV
                     GHTWRVTNFALAIARELGWDHEKLRECEMGGVLHDVGKIAIDDNILRKPGGLNDDEYE
                     KMKVHPERGARMMQDVPALASLIPYALYHHERYDGEGYPFGLTGEEIPIEGRLIAVAD
                     TFDAMTSNRPYRKGLSPEIAVAELEKGKGTQFDPVIVDAMVRAYRAGRVDAIFQNYAK
                     DDLSVSCPFCSTYVPVPETGEAGTEFKCGVCHRRLRLREQNDVYFAELLPTTEASPVD
                     LNYRTPRPATQAMDETAR"
     gene            5684..6955
                     /locus_tag="PKW60_00550"
     CDS             5684..6955
                     /locus_tag="PKW60_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97947.1"
                     /translation="MARWLERFRTMPWRPARRKTTVLAETFRERPPRTYTVPEDASRL
                     QGALRDVIGLPNETPFYMLQVYRFLRDAIPDISDAIWTWKRLCQTGYDLEITQASSRP
                     AQRRAERLLDALNRRVNSGDRGMDGLLDIFYTSLFTYGAAAVEIVLSQSRESIWDVVP
                     VDVWTLRFRRGKHGLEAFQLHEGREIPLPRERFMYVGLDRDGTNPYGRSMLRAIPFVV
                     KIQQRLLEDMAKATHNAGWAKLHVQYRPEERQRGESVEAYHNRVEANFSELRTKLSSL
                     ETDQNLVTFDNVSVSLVRGDQRSQVFYENHKAIEEQVITGMHLMPILLGRNYGTTETY
                     GTAQFEIITRQVTAVNRCVKRLLERLYNFELALMWGSARAAVHMRSHRTADIVREAQA
                     RRHEIENAILLRDAGFLTHEKAAESLGITVS"
     gene            6983..7672
                     /locus_tag="PKW60_00555"
     CDS             6983..7672
                     /locus_tag="PKW60_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97948.1"
                     /translation="MELEPFVFRHEGVLVGTRDGGTVADTIDEVNAFTLRPLTAEEFA
                     VFTLDLCNNRIDRHFSRFPEEELERINALVPGRPLMERHDVRGSLPRGTFFRSRLHTD
                     GEQLAVRPDVYVLRTRANQDFILNIEGGVYRETSIGFSFRKPECSVCGKDLRTCEHIP
                     GRSYGENLCHCVLRDVLEVTEGSVVASGSQGTRFVAGTRALGLDDALAAARRDFHLPI
                     ELGAGGLWEDE"
     gene            7669..9330
                     /locus_tag="PKW60_00560"
     CDS             7669..9330
                     /locus_tag="PKW60_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97949.1"
                     /translation="MMRRYPWLFSIGIAAVLLPALGAAPRYLESLRIGGGYGDSDGGV
                     DIDADGDIRTDGGIILRGRLVAGPEEHTLTTSEGLLDAGALCIPAAQSESQADDADMV
                     LIHDASAGATRNMTRADFLSGTGEQLWKDTGSAIQPLNAPDLRIQNSGDVILPGALNV
                     RGPVSAGTATGNSYLLKAQFEGSSTLSSTSRAALEGHTVWSSGFHTASSIQGMAGSAT
                     IGSGYSASATDLIGAAGRVMPGPVFTSINRGVGVRGEVLLNQLGGMTNGVALHGRVSG
                     ATGTVTNLYGLYLDDITQGMAANYAVYTGRGACRFGDDVTVAGDLEVQGGDLTTPGNL
                     NVSAASGAATLTLASSNFSVSASQLQQQGQIRLHNSTQPALYLDRTGTPVKDTAVGSI
                     VFRGLNSASSPVSYALISSRIQETAAGSESGQIEFRLLNAGTQTTALRLNGPDAFFGA
                     NASVAGALNTSALVTFAANDSTPSVRGANVFCVPDGWTPGHDVTQLEDGAPGQFVTIV
                     GGDSDCVFADNANLHLAGPWTAHPDDTLVLVFVAGAWYEISRSSN"
     gene            9345..9569
                     /locus_tag="PKW60_00565"
     CDS             9345..9569
                     /locus_tag="PKW60_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97950.1"
                     /translation="MNPCSNAAGKQALPDSAAACPYQPVHDIQWMSLREDLCEVKTRV
                     QRLESTLARGVALLVANLVGVIMSLLRQLA"
     gene            9590..10294
                     /locus_tag="PKW60_00570"
     CDS             9590..10294
                     /locus_tag="PKW60_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97951.1"
                     /translation="MYWKIAFLPEMSPVSERNQAPAGRFVLHRHVDAAGPHRDLRLEQ
                     AGYLVGWRFEQAPCPEGVWATEKAPHPLRWLEHDGDAVREEAGLFTVIRWSKDVRELL
                     LEGRHSTQFLRAERMPSFSPDTARALHETIREMALEPTALAGIVRDGVTARQRALERF
                     CGLGRELDAAAFDEGVWRRTLESLSLEEITRHLRAYEVRFDNKFPPSPTSQPEALPDS
                     EPAAWTDRAMGIVRGG"
     gene            10433..10816
                     /locus_tag="PKW60_00575"
     CDS             10433..10816
                     /locus_tag="PKW60_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97952.1"
                     /translation="MAFGDIGGVVTELVITCKTPDSGSVNIAKGDAVSLAGPYTVCND
                     ADAESPVLGEALAGSTANGAAIPVKVRGVSVFDYEGETPPTVNGLAGIVAAESSGKVK
                     APASGNGAGINLKVDTVAGQVHVLL"
     gene            10830..11876
                     /locus_tag="PKW60_00580"
     CDS             10830..11876
                     /locus_tag="PKW60_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97953.1"
                     /translation="MAYRDDIVKERVAFLSQEPGQLRQIRGAGLYETLQGLGLNHGQF
                     FRALGTTVRDYCAQEFGIDLDRITVDRFFQSDPNAKWLFPDMVRDAVVSGMTRKPVYP
                     SLIIRDERVASTAFDVPYVREDELEEELRTVAEGAAIPESSIKYGDRVIKLDKKGRGV
                     IASYEVIRRMSVDMLRVHLQRIGERLGRHLDARLAQILAEGDGAAGTAPVTLDTATAD
                     TWAYGDLVNGFMTLTLDHYFTPTHMLANADLCQTILELDAFRESALFDFTKTGNLPTP
                     LGTRLVPMAGQPANRLTILDAGYAAQKLTEQELLVESDKLINQQWDRTYLTVVTDFAI
                     IYEKARVVIRSDWS"
     gene            11943..12362
                     /locus_tag="PKW60_00585"
     CDS             11943..12362
                     /locus_tag="PKW60_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97954.1"
                     /translation="MSGFTTETMVRLRFQLQNTASVPAELIEAAIDDAHAELLTRLKP
                     GFTEAPLPHLVVTGETLLAGAEVYRALAAQDAHSQVHVAVGGQRVEAGRRFDALMAVA
                     RSARTAAWDLLAPFLRDHPPHAPADVTDTIPILGGSD"
     gene            12359..13048
                     /locus_tag="PKW60_00590"
     CDS             12359..13048
                     /locus_tag="PKW60_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97955.1"
                     /translation="MMAITLNGLLLDEMLTSIQEQHEEVGGRDARTVKIAGVLLGETS
                     VAALEARLDAVLHAASAVDYSAALSIRPGRRLMVRRAAFRREIQHARLVGMFTLKLEA
                     RDPFEEAESLTEIAWPITASPSSLLVNSGGTACALPRIMLTAAGALVRPTFSDGVQCL
                     EYDGIVKEGSFLEFDTARARATLDGEDVTPYTWGAFPRLEPGETTLTYTDQSTSSHQA
                     AATVAFRERWW"
     gene            13051..14787
                     /locus_tag="PKW60_00595"
     CDS             13051..14787
                     /locus_tag="PKW60_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPJ97956.1"
                     /translation="MRYAIEIYNQQGLRIARFHSVPLMDATRSAPDQPDRIEGLLPEA
                     VDGLGPGCRIAIRLEDELFCEAEVTWVGPQWSDTKKLILDRYVNFHEVIAIRAERRNT
                     RQNTHVTRGYGPERLDAIVKDLINTAVGPVHYLVTHTAYPDGAEREYGKYLARKTPAN
                     ELELGGISAGQWVETPRIDASRAYAKDGDTIAGLSVDGVAWPDLRLMMIDCEELTRNS
                     HAVARHPEIASWSSARYAASGYALAGEAARRALQDLIDSKGIRAIELNPHRGASGAYD
                     DRIDAYGRYVGLVYGGGECFNAAMVELGHSEVYQYKDGRYHAPEMRLKEYFSYTRASS
                     DSIEAVPVTLNAFEADHGLFDLLTALAYAGGCVWNLSPHDAVHFRPAGICDHVLAYDP
                     LTIGVQLASDGASIVNALYFEGNPILGPVKKTYIRGESADTFGFRARRFRHFGIASEG
                     DADRLANGLLHDLAYPEAAGFLTLYHGAAAFQAGDLLELRGAPPRRLDLPLPGEWGGQ
                     FAGRLVRRVNRVTHRFSGRTVTTTLGCTSPLRSVANPLYHMMQHQGPEQSLYQFRLDD
                     PAVGLDSGYHLE"
     gene            14831..15352
                     /locus_tag="PKW60_00600"
     CDS             14831..15352
                     /locus_tag="PKW60_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97957.1"
                     /translation="MAIRGKSFIKTSWAFQERPVASAKLNTWDDRIEAALELLMNLIS
                     LAWGGGNGILRNAADGDLGISAISPPALAVQVAPGYAFIERFPFHLKTATVLGGIAAP
                     LHHPRIDLVQARLDTWDVTITPGVEAVSPSPPGPGAACLALAHLHLRPGMTAVVNTDD
                     GLNGYISDVRTYV"
     gene            15333..15809
                     /locus_tag="PKW60_00605"
     CDS             15333..15809
                     /locus_tag="PKW60_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97958.1"
                     /translation="MSALTYNRRNDVRLLTNLDGREVSHFRLREFENAAGLAMVHSTL
                     LESLERVRRDLCEMAGETVWILVTSAVRTEEDLRRLAALYGWTDQGGAVSRNSRHLAK
                     FGGIAADIVAVLERNRARVPQKTLGAVCRRYFDWVKDDYNDGHVHADNRHRAAPPT"
     gene            15827..16192
                     /locus_tag="PKW60_00610"
     CDS             15827..16192
                     /locus_tag="PKW60_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97959.1"
                     /translation="MTFNDAIHSDQGLMLLGVVLGGLWSLFKSTDLYARARRLRCERA
                     LLALETGVMNTYETYVREIKASREDGKLTSAERKEARRRAKEYAIELARVEGLDIARE
                     LGHSYLDAWLERLVRQVKS"
     gene            16368..17354
                     /locus_tag="PKW60_00615"
     CDS             16368..17354
                     /locus_tag="PKW60_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ97960.1"
                     /translation="MLRVALVGAGNFAARHYAALRGFPERVEVALVARNQCDTPFEPA
                     PQLPVLGFDAVLADPSIDAVVISSPNGLHCSQAEAALHAGKHVFCEKPLALCGHAARA
                     VAGAAARAGRVLMAGHLTRHTPLYAAAAEIVRSGGLGQVRTIHAARWQTSDKGRDWRM
                     QPGQGGGAPFDLLLHDYDLLQWLCAVPESVVATGHKHPSGAYQRLTASFACPDGVAAT
                     VDGGFVLSPGETMKSSLHVAGTEGVLDIQTPGEDPILLRQEGGEPEPVSIDPDRMGVD
                     GLRTEFLEFFDAIDGQAWNRLRLDDACNAVAIAAAALAAAETNCRVDLTTGM"
     gene            complement(17341..18108)
                     /locus_tag="PKW60_00620"
     CDS             complement(17341..18108)
                     /locus_tag="PKW60_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HPJ97961.1"
                     /translation="MAGDEITRAEIARGFREAGLRSGEAILVHSSLSSFGRVAGGADA
                     VLDGLLDALGPEGTLLAPTLTGGPELSPENPPHIDLRTAPCWVGRIPETLRQRPDAVR
                     SVHPTHSCAALGRRAAELTRGHYLSPTPCGVTSPYFRLALAGGTIVMIGCDLSRCTTC
                     HTAEELANVDYHLQPRVTHGSCIDQCGVRIETPCRLHRYDGPERNFPALEPLLLEAGL
                     MRKDRVGNAEIRLIDAMGLIETALDRLRFDPYFLTSR"
     gene            complement(18127..>19111)
                     /locus_tag="PKW60_00625"
     CDS             complement(18127..>19111)
                     /locus_tag="PKW60_00625"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPJ97962.1"
                     /translation="DTAGKRFYVWFDNAIGYVTNTRQYYLDRGEPDGWERWWKDPASR
                     LIHFIGKDNVPFHAVMFPTYLMGQGDYILADNVVGNEYLNILNRETGKTEKGSKSKGN
                     MISVRWFTSRFSTDAIRYYLCSIMPESRDAAFDWDDFYGRYNGELSNVVGNFIHRSLT
                     MTVKNFESRVPEPGPLDDGDRAMLAAIPEQLDGVAEALENFRFRQGLERFVDLARKAN
                     VYFDGKQPWMTRKTDLGRTATTLYVCCQVVRALCVAMAPFVPDGAQQLAKVIGISLPE
                     GGSDGGADGWNAGRDGLAAGSPLQEPEVLFPKLDKDYTAECADAHMRGEAF"
BASE COUNT         3533 a         6269 c         5849 g         3460 t
ORIGIN      
        1 ttccccggct gatggcctgg tatttgaaca tgtcccacca ctggccgtat ccgggctgag
       61 ccgggtcgcc tttctcgcgg ataaacgggt tgggagcctc cagataggag tcgagcggca
      121 cgtaccagcc tttttgcaca tcgacccaga catcttcgac gttgaccatc aggatgtcgg
      181 gggcgctgcc gctgctgagc tgggtgacga gatactcgcg cacgccgggc acggtgatga
      241 gttccacccg tgtgtcggga aaccgggcct caaaatcccg caccacgtcc gcaatgccgc
      301 ggagcggttc acccagcccg aacggggtcg tgccgggcat atactgttga ccggggacga
      361 atttgatgac aaaccggggc tccgcgccgt tattccggtg ctccggcgca cccgaggcga
      421 actcgccgcc ggcccggggc cacaggagcc acacgctccc cgcgataacc accgccagcg
      481 aagcccgtct gaaatatgtc ccccaagacg ccatcttgcc tgtcatgata gccgatccgg
      541 ggtcctgaga aaaccctggc cgaccccggt tctccatgct ggagcacgca agcgggccgc
      601 tggcgttcgt aatcgtcccg agtttaccgc cggcgtttca tccggcgccg cggccgcggc
      661 atgggggccg cgggccccgg tgctttctgc cgcgcaggtt tggcgcatcc gcaccgggcc
      721 ttactcgaat tcatcgtctt cttcgtcctc gtcatcacct ggtcccaggt tggccacgtc
      781 ctgacccgcg atttcgagga cttcctcggc cgcgaagata gggcagccta cgcgggtggc
      841 aatggcgatc ccgtcgctgg gccgtgagtc gatccggagg acctgctcga cctggccgtc
      901 gacggactgc tgctcaacgt taagatgggc gtagaaggtc tcgttttcga gtttatagat
      961 cgtgacggac ttcagctcgc cgcgcagccc agccagcaca ttgcagatca ggtcatgggt
     1021 catgggccgc cccagatcct ggtggatcat gccggtatgg atggcctgcg cctccgcccg
     1081 gcccacgacg atgggcagga cccggtcgcc atggcgcagc agcacaaccg gcggatggtg
     1141 ctcttcgctc aagctgaccc ccagaatatc catttcaagc attacgtcct cctcttcgga
     1201 ccctgttatc gcctgttccg ctgccggttg actgtctggt ttggagtgta cgcgcccccg
     1261 gataccttgt caacgacaga acgaggggct ggcggggtga agtgcgctca ggaaaacgac
     1321 ttcgtgcccg cttccggggt ggcggatttt gaggaattga tcacgaaaat gaccaaagcc
     1381 atcgacaacg ccaggcgcgt gacctgttgc gcctgggcgt cgcagtgatg tatctgtatt
     1441 cagacaaacc cgtcaccctg ccgatctctc acggcgttac cgggtacggg cctgtcagca
     1501 gccggcaggt tattttgctg ccgcgtcagg cacaaccttc cggtaattgg gctcggcctg
     1561 ggggtctccg gtacccatga gccagtgcac ggcgtcgacg aaggcctgct ggaactgttg
     1621 gccttcccag aggtcctcgc ggtgccccag cgcggtgaaa tacacgcggc cctgtcccag
     1681 ggcgcggatc cagatgaccg gatacggggc gatgttgtac atttcctgtt tcgcgcgttc
     1741 cgcaccggga tccatgaggg ccagcacatg aatcgcatcc acattgaact tgtggaacaa
     1801 gtaccactcc tcgttgagct cccagccgtc ggggatacgg gccatggccg gatgctgggg
     1861 atcgaccacc tgcaccttgc ccacaaattg ctgcccgtgc gtcttgaatt ccgcgccgat
     1921 catggagatg tacggcgtga tcacgtcgcc ctcgacgtca aatgtgtccg ttgcggaatg
     1981 aaacccgagg aagcggccgc ctttctcgat ccattggatc agttccgtct ggccgttcgg
     2041 ccccatgggc ggcgtattgg tcatattggg ccgggtgagg tccccttggg tgtaaaacat
     2101 gacgaggtcg taatttttga ggttttccgc gttgatggtg ctggcgtcgt acgtcaccgt
     2161 caggctggcc cccatcttct cgagaatggg ccgcaggatc ttctccgcaa gcggtggttc
     2221 gccgtctttc gcgacggtcg ggctgtgggc aaagccttcc gacgtcagta gaaaaagcac
     2281 gtttttaggc acgggttgct cggcgccgag cgctgcctgg caacccagca ccgcgatcat
     2341 tccagccaag gctgttcgca tcatcatgtc agttctcctt ccgggtatgc agggcacggg
     2401 gaaaaatcac ctgtccgcaa gcccatcatg tgggttcaat ataccgctac cgaaaccggg
     2461 tccgccacac gcggtcagct cagtgcgcct accgccaccg cgcccagcaa tccccgctgt
     2521 ttattgaggc gttcctgaat ccgcgtcaca agaccctcat ccagggggag tctttcgagc
     2581 gactcgggat tgggagacag ggcttgcggc tgatcgttca gcgcgccgat gcccatcatg
     2641 ttgaccagcg cgtccgccaa atgagtcgca tgcggcatga tcggattgcc cggcacatcg
     2701 gacgggcggt gagtcccggc gatactctgt accagcactt cgggcaactg ccatcgttcc
     2761 gccagccacg cgccgatttc accatgggtt atgccgccga acacttcgta ctctgcttcg
     2821 taagcggagc agcccaggga ctccaccata tggatcaccc gagtcagttc gtcgggaaaa
     2881 tactggataa tcaccgcctt gccgatgtcg tgcagcaagc ccgcgagatg cgcgcccgca
     2941 atctcggggt acagccggcg aaaatgctgt tccagcgttc cggctgccag cgaacaggcc
     3001 agactgtgcc gccagagatt gtgcagggca cgtacatggg ccgggctctt cccgaagatg
     3061 ttgatgacgg agatggtgag ggcgagccgc tcgatttcgt tgaagcccag tatcaccacc
     3121 gcttcgggga tgtttgaaat ggcccgcgga aagccgtaga aactggagtt caccacacgc
     3181 aggacgcgca tcgccagagc ttgatcacgc acgatctcgt cggccagatc cagggcggtg
     3241 gtgctttcat cctggaccac ggcgatgatc cgggtcaaaa cctcgggcag ggtcgcgata
     3301 tcatcgattg cttcaacgcg gcctcgcatc tgaatcctcc ctgacaaacc ccagggatct
     3361 cacacgaccc gctctcacag acgtgagcgg gaaaactcag atttcccgct attgacactg
     3421 cgattatagg aactcgccag acacctgtca actaaacgga cgatcgcatg cgattttccg
     3481 cgaatcttgg cagggttgtg tgctataaag gaggggaaat gcggaaagaa agcctttccg
     3541 gcataatctc aaggtgcgga ttagtgcagg gcgtggcagc ccatccgccc ggcttcgcgg
     3601 tgcgaggtgt ggcgtgcggg caggtcttcc aggccctgga caagcgcggg aatgtgtaaa
     3661 ctccggaccg tggacgactg aatgaaaccc tgcgagacaa tccgagttct gaccggcctc
     3721 aggaagggtg aaacgtgccc aatcacaggc acgctgacca ttggccggag cccgcacagc
     3781 ggcctgcaac tcaacgagct cgaagtgtcc cgcaggcatg cggtgattca acagacggcg
     3841 ctgggcacgc ttatccggga cctggggagc ggcaacggga cgtatgtgca caaccaccgg
     3901 attgtcgagt accggctgtc tcacggcgac gtcatttcca tcggccccat tagtctgcgg
     3961 tttgaaagct gtgcggcgga attgccgagc cattcagggt cgccctccgt ggtgtttgag
     4021 gaaggcggcg tggagaatgt gcaggccctg accgcggaaa acgttcaccg cacgtttctt
     4081 gccgctcctc tgtccgcgca gaccgcagag caggcccgca aggcccagga gcgccttgcg
     4141 gcggtttatg cggccaatca ggttatctca agcgagcgcg atctgaaccg gctttttgag
     4201 cgcgttctcg accagatctt tgggctggtg ccggcgcaca acggggctat tctgctggcc
     4261 gaccccgcca catcgcgcct ggaacctgcc cacatgcgca agggccgcga cgaaaacgag
     4321 cacgtgacga tcagttccac catcgtgcag cgggcctacg atcagagcga agccatcctc
     4381 gtgtttgacg ccgcggacga cagccgtctg ggccacggcc agagcattat tctgcacaac
     4441 attgcctcgg ccatgtgcac gcccctgcag gtgcacgatg agacgctggg cgtgctctat
     4501 gtcgacacac gaggcaccac gaatgcgttt acggagggcg atctcgagct cctcgtggcc
     4561 cttgcgggac cagccgctat cgccatcaaa aatgcgcaat acgtcgataa gctcgagagc
     4621 gcatatcagg gcaccctggt catgacggct aacgccatcg aggcccgcga tcattacacg
     4681 gtgggccaca cctggcgcgt caccaatttt gccctcgcca tcgcgcgcga gctcggctgg
     4741 gaccacgaaa aactccgcga atgcgaaatg ggcggcgtcc tgcatgacgt gggcaaaatc
     4801 gccatcgacg acaacatttt gcgcaaacct ggcggcctca acgacgacga atacgagaaa
     4861 atgaaagtgc atcctgaacg cggggcgcgc atgatgcagg atgtgcccgc tctcgcctcg
     4921 ctgatcccgt atgcgctgta tcatcacgaa cgctacgacg gcgagggcta tccgttcggc
     4981 ctcacgggag aagagatccc catcgagggc cgcctgattg cggtggccga caccttcgac
     5041 gccatgacca gcaatcgccc gtaccgcaaa gggctcagcc cggaaatcgc cgtcgcggaa
     5101 ctcgaaaaag gcaaaggcac ccagttcgac cccgtgatcg ttgatgccat ggtccgggcc
     5161 taccgggccg ggcgcgtcga tgcgatcttc cagaattacg ccaaagacga cctcagcgtg
     5221 tcgtgtccgt tctgcagtac gtacgtaccc gttccggaga ccggcgaggc gggcaccgag
     5281 ttcaaatgtg gcgtctgcca ccgccgcctg cggctccgtg aacagaacga cgtctatttc
     5341 gccgaactgc tgcctacgac cgaagcatcg cccgtggatc tcaattaccg cacgccccgg
     5401 cccgccacgc aggccatgga cgaaacagcc cgctgactcg cctggcgcgg cacggcactt
     5461 cgctggccgc ccacgcccgg accggatccg cagagccttt cccctctgag ttcgattgaa
     5521 cctccgtggc cggctgctgc tcttacggag tacatgcaca ctggaggcac tccgtcctaa
     5581 gatacgtcac ttcagcatct tccagagcga atacgtttcg ctgccgggac aatacacgct
     5641 ccaatgtagt agacgcggcc gcaacccccc gcaaggagcg aacatggcac gatggttgga
     5701 gcgatttcgg acgatgccgt ggcgcccggc ccggcgcaaa accacagtgc tcgcggagac
     5761 tttccgcgaa aggccgcccc ggacgtacac ggtgccggaa gacgccagcc gcctgcaggg
     5821 ggcccttcgg gatgtgatcg ggctgcccaa cgagactccg ttctacatgc tgcaggtcta
     5881 tcgctttctt cgcgatgcca tccccgacat ctcggatgcc atctggacct ggaaacggtt
     5941 gtgccagacc ggctacgacc tcgagatcac gcaggcctca tcgcgcccgg ctcagcgccg
     6001 ggcggaacgc ctgctcgatg ccctcaaccg ccgcgtcaac agcggcgacc ggggcatgga
     6061 cggcctgctc gacatcttct acacctccct gttcacgtac ggggcggcgg cggtcgaaat
     6121 tgtgctgtcg cagagccggg aatcgatctg ggacgttgtg ccggtggatg tctggacgct
     6181 gcggttccgg cgcgggaaac atgggctgga ggctttccag ctccacgaag gccgcgagat
     6241 ccccctgccc cgggagcgtt tcatgtatgt gggcctcgat cgcgacggca ccaatccgta
     6301 tgggcgatcg atgctgcgtg ccattccgtt cgtggtcaag atccagcagc gtcttctgga
     6361 agacatggcc aaggccaccc ataacgcggg atgggcgaag ctccacgttc agtaccggcc
     6421 cgaggaacgg cagcggggcg aatcggttga ggcctatcac aaccgcgtag aggccaattt
     6481 ctccgaactg cgcacgaagc tgagttccct ggagactgat cagaatcttg tgacctttga
     6541 caacgtcagc gtgtcgctgg tccgcggcga tcagcgctcc caggttttct atgaaaatca
     6601 caaggccatc gaggaacagg tcatcacggg catgcacctg atgccgatcc ttctggggcg
     6661 caactacggt acgacagaaa cctacggcac agcgcaattc gagatcatca cccgccaggt
     6721 tactgcggtg aaccgttgcg tcaaacgcct gctcgaacgg ctgtacaact ttgagctggc
     6781 cctcatgtgg ggcagcgcgc gtgccgccgt gcacatgcgc tcgcaccgca ccgccgacat
     6841 cgtgcgcgaa gcccaggccc ggcgccacga gattgaaaac gcgattctgt tgcgcgacgc
     6901 cggcttcctc actcacgaaa aggccgcgga atccctgggc atcactgtct cctgaacccg
     6961 tatcccgagg aaaggaactc caatggaact ggaaccgttt gtcttccgcc acgaaggcgt
     7021 cctcgtcggc acacgcgatg gcggcacagt ggccgatacg atcgacgagg tcaatgcgtt
     7081 taccctgcgg cctctgacgg cggaagaatt cgccgtattc acgctcgatc tgtgcaacaa
     7141 ccggatcgac cgccatttca gccgctttcc cgaggaagaa ctggagcgga tcaacgcgct
     7201 ggttccgggg cgccccctca tggaacggca tgatgtgcgc ggttcgctgc cccggggcac
     7261 gttcttccgt tcgcgccttc ataccgacgg agaacagctg gcggtccggc ccgatgtcta
     7321 tgttctccgt acccgcgcca atcaggactt catcctgaac atcgaaggcg gcgtgtaccg
     7381 ggagacctcg atcggtttct cgttccgcaa acccgaatgc tcggtctgtg gcaaagatct
     7441 gcggacctgc gaacacatcc ccgggcgcag ctacggcgaa aatctatgtc attgcgtgct
     7501 gcgcgatgtg ctcgaggtaa ccgaaggctc cgtcgtcgcc tcgggttcgc agggcacccg
     7561 cttcgtggca gggacccggg cgctcggtct cgatgatgcg ctggctgcgg cccggcgcga
     7621 ctttcatctg cccatcgagc tgggggcagg cggactctgg gaggacgaat gatgcgcaga
     7681 tatccctggt tattcagcat aggcattgcg gccgttctgc tgccggccct gggcgccgca
     7741 ccacgctacc tggaatcgct gcgcatcggt ggcggctatg gcgattcgga cggcggagtc
     7801 gacattgacg ccgacgggga catccggacc gacggcggca tcattcttcg cggccgactg
     7861 gtggcgggtc cggaggaaca tactctcacg acgagcgaag ggctgctcga cgctggtgcg
     7921 ctctgcattc cggccgccca gagtgaatcc caggccgatg acgccgacat ggtcctgatc
     7981 catgacgcca gcgctggcgc cacgcgcaac atgacgcggg cggatttcct cagtggaacc
     8041 ggcgaacaac tgtggaagga taccggcagc gcgatccagc ctttgaacgc cccggacctc
     8101 cgcatccaga acagcggcga cgtcatattg cccggggctc tcaacgtaag aggcccggtt
     8161 tccgctggaa cggccaccgg caattcgtat ctgctgaagg cgcagtttga aggttccagt
     8221 acgctctcct ccaccagccg tgccgcactc gaaggccata ccgtgtggtc cagcggattt
     8281 cataccgcat ccagcattca aggcatggcg ggcagcgcca ccattggctc gggatacagc
     8341 gcttcggcaa ccgacctcat tggcgcagcc ggacgcgtga tgccgggacc ggttttcacc
     8401 tcaatcaacc ggggagtcgg cgtccgcggc gaagtgctgc tcaaccagct cggcggcatg
     8461 accaacggcg tcgccctgca tggccgggtc tccggcgcca caggcacggt caccaacctc
     8521 tacggcctgt atctcgatga catcactcag ggtatggcgg ccaattacgc cgtttatacc
     8581 gggcggggcg cctgccggtt tggcgacgac gttaccgtcg cgggagacct cgaagtccag
     8641 ggcggagatc ttaccacgcc gggcaacctc aacgtttctg ccgccagcgg cgcagcgaca
     8701 cttacgctgg cttcctcgaa tttctcagtc agcgcatcgc aactccagca gcaaggccag
     8761 atacgccttc acaacagcac gcaaccggcg ctgtacctgg accgcaccgg tacgccggtc
     8821 aaagacaccg cggtgggttc gatcgtattc cgcggactca acagcgcatc cagtcctgtg
     8881 tcatacgcgc tgatttccag ccgaatccag gagaccgctg ccggcagcga atcgggccag
     8941 atcgaattcc ggcttctcaa cgctggaacg cagaccacgg ccctgcgcct gaacgggccg
     9001 gatgcgttct ttggcgcgaa tgcgtctgtg gccggggcgc tcaacacctc cgcgctcgtc
     9061 accttcgccg ccaacgacag cacccccagc gtacggggag ccaacgtctt ttgcgtgcct
     9121 gacggctgga cgcccggtca tgacgtcact cagctcgaag acggagcccc cgggcaattc
     9181 gtcaccatcg tcggcgggga ttctgactgt gtgttcgccg acaacgccaa ccttcacctg
     9241 gcggggccgt ggaccgccca tcccgacgac accctggtgc tcgtatttgt ggccggcgcc
     9301 tggtacgaaa tcagccggtc gagcaactga aaggaggccg cgcgatgaat ccgtgcagca
     9361 acgcagcggg aaaacaggca ctgcctgaca gcgcggcagc gtgcccctac cagcccgtcc
     9421 atgacattca gtggatgagc cttcgggagg atctctgcga ggtcaagacc cgcgtgcagc
     9481 ggctcgaaag cacgcttgcg cggggcgtgg ccctgctggt ggccaatctg gtgggcgtca
     9541 tcatgtcttt gctgcgccaa ctggcgtaac gagaagggag actgtgggca tgtattggaa
     9601 aatcgccttt ttgccggaaa tgagcccggt ttcggaacgg aatcaggcgc ccgcggggcg
     9661 ttttgtgctc caccgccacg ttgacgccgc aggcccgcac cgggatctgc ggctcgaaca
     9721 ggcgggatac ctggtcggct ggcgttttga gcaggcgccc tgcccggaag gcgtctgggc
     9781 tactgaaaag gcgccgcatc cgctgcgctg gctcgagcac gacggcgatg ccgtgcgtga
     9841 agaagccggc ctgttcactg tcatcaggtg gtcgaaggac gtgcgcgaac tcctgctcga
     9901 aggccggcat agtacgcaat ttctccgggc agaacgcatg ccgtcgttct ctccggacac
     9961 cgcgcgcgcc ctccacgaaa ccatccgcga aatggcgctc gagcccacgg ccctggccgg
    10021 catcgtgcgc gatggcgtga cggcgcggca gcgtgcactc gaacggttct gcggacttgg
    10081 gcgcgagctc gacgccgccg cctttgacga gggggtctgg cggcgaacgc tggaatccct
    10141 cagtctcgaa gaaattaccc gccatttgcg tgcgtacgag gtccggtttg ataacaaatt
    10201 tcccccatca cccacgtcgc agccggaagc gctcccagac agcgagcctg cggcctggac
    10261 ggaccgggcg atgggtatcg tgcgcggcgg ctgaacgccg gccgcgccac ggcgctctct
    10321 gtcccggctc caacagcccg ggcgaagggc cggttcctga cacgaacaac ctggccgcac
    10381 ataccgtcat gaatcgctac ggctgcgcgg ccgaacccca ggagtgacca tcatggcatt
    10441 tggagatatt ggcggcgtgg tgaccgaact cgtcatcacc tgcaaaaccc ccgattccgg
    10501 ctccgtgaac atcgccaagg gcgatgccgt ctcgctggcc ggaccgtata ccgtctgcaa
    10561 cgacgccgat gccgaaagtc ccgtgctcgg cgaggccctc gccggcagca ccgcgaatgg
    10621 cgcggccatt ccggtcaagg tgcgcggcgt atccgtcttt gactacgaag gcgagacccc
    10681 gcccacggtc aatgggcttg cgggcatcgt cgccgcggag tccagcggca aggtcaaggc
    10741 gccggcatcc ggcaacgggg ccggcatcaa cctcaaagtg gacacggtcg ctggccaggt
    10801 ccacgtcttg ctctagggag gaatcaacga tggcgtatcg cgacgatatc gtgaaggagc
    10861 gggtcgcgtt cctgagccag gaacccggcc agctccgcca aatccgtggc gcgggacttt
    10921 acgagaccct gcaggggctc ggcctgaatc acggacagtt tttccgcgcg ctcggcacga
    10981 ccgtacggga ctattgcgcc caggaattcg gcatcgacct ggaccggatc accgtcgacc
    11041 gcttctttca atctgacccc aacgccaaat ggctgtttcc ggacatggtc cgcgacgccg
    11101 tcgtcagcgg catgacgcgc aaaccggtct accccagcct gatcatccgc gacgaacggg
    11161 tcgccagcac ggcctttgac gtcccctacg tgcgggagga cgagcttgaa gaagagctgc
    11221 gcaccgtagc ggaaggcgcc gcgatccctg agtcgtccat caagtacggc gaccgcgtga
    11281 tcaagctcga caagaaaggc cggggcgtga ttgcatcgta cgaagtgatc cggcgcatgt
    11341 ccgtcgacat gctccgcgtt cacctgcagc gcattgggga gcgtctgggc cgccatctgg
    11401 acgcgcgcct tgcgcagatc ctggccgagg gcgacggcgc cgccggcacc gcgccggtca
    11461 cgctcgatac tgccacggcg gacacctggg cctacggcga tctggtcaac gggttcatga
    11521 cgctcaccct cgaccactac ttcacgccga cccatatgct ggccaacgca gacctctgtc
    11581 agaccatcct cgaactggac gcgttcaggg agagcgccct tttcgatttc acgaagaccg
    11641 gcaatctccc cacaccgctc gggacgaggc ttgtgcccat ggccggccag cccgccaacc
    11701 ggctgaccat cctcgatgcc gggtatgcgg cgcagaagct gacggagcag gagctcctgg
    11761 ttgagagcga caagctcatc aatcagcagt gggaccgcac gtacctgacc gtcgtcaccg
    11821 atttcgcgat catctacgag aaagcccgcg tagtgatccg gagcgactgg tcctgacgta
    11881 acgggggcgg tccgcgtccc acgggccgcc cccgtctccc gcaccgataa ggaggttccg
    11941 acgtgtccgg ctttaccacc gaaaccatgg tccggctgcg atttcaactc cagaacacgg
    12001 cgtctgttcc ggccgaactc atcgaagcgg ccatcgacga cgctcacgcc gaactgctta
    12061 cccggctgaa acccggtttt accgaggcgc cgttgcccca cctcgtcgtc accggtgaga
    12121 ctctgctcgc tggcgccgag gtctatcgcg ctctcgctgc gcaggatgcg cacagtcagg
    12181 ttcacgtcgc cgtaggcggc cagcgcgtcg aagccggccg ccgttttgac gcgctcatgg
    12241 cggtcgcgcg cagcgcgcgc accgcggcct gggatctcct ggcgcctttc ctccgggacc
    12301 atccgccgca tgccccggcc gacgtcacgg ataccattcc catcctcgga gggtccgatt
    12361 gatggcgatc acgctcaatg gcctgcttct cgatgaaatg ctcacctcca ttcaggaaca
    12421 gcacgaggaa gtcggcgggc gggacgcgcg aacggtaaaa attgctggag tgctcctcgg
    12481 agaaacgtcc gtcgctgcac ttgaggcccg gctcgatgcc gtactgcatg cggcgtcggc
    12541 ggtggactat tcggcggcgc tgtcgattcg cccgggacgg cggctgatgg tgcggcgagc
    12601 ggcgttccgc cgcgagattc agcatgcgcg tctcgtgggg atgttcacgc tcaaactcga
    12661 agcgcgcgac ccgtttgaag aggccgaatc gcttacggaa atcgcctggc cgatcacggc
    12721 atcgccttct tcgctgcttg tgaacagcgg cggcaccgcc tgcgccctcc cccggatcat
    12781 gctgacggcc gccggggcgc tggtccggcc aaccttcagc gacggcgttc aatgcctcga
    12841 gtacgacggc atcgtgaagg agggctcctt cctcgaattc gacacggcgc gggcgcgagc
    12901 cacgctggat ggcgaagacg tcactccata tacctggggc gccttccccc ggcttgaacc
    12961 cggcgagacc acgctcacct atacggacca atccacgagc tcacatcagg cggccgccac
    13021 agtcgcgttc cgtgagcgct ggtggtgacc atgcgctacg cgatcgagat ctataaccaa
    13081 caaggcctcc ggatagcccg cttccattcc gtgcccctga tggacgcaac ccgcagcgct
    13141 cccgatcagc ccgatcgcat cgaaggattg ctccctgaag ccgtcgacgg gcttggcccc
    13201 ggttgccgca tcgcgatccg gctcgaagat gagctgttct gcgaggccga agtcacctgg
    13261 gtgggtccgc aatggagcga cacgaagaaa ctcatactcg accgctacgt aaactttcat
    13321 gaagtcatcg caatccgggc cgagcgccgc aacacccgtc agaataccca cgtgacccgg
    13381 ggctacggtc ctgaacgcct cgatgccatc gtcaaagatc tcatcaatac cgccgtggga
    13441 cctgtgcact atctcgtgac ccataccgcc tatcctgacg gcgccgagcg cgaatacggc
    13501 aaatacctcg cccgaaagac tcccgccaac gagctcgagc tcggcgggat cagcgccggg
    13561 caatgggttg aaacaccccg catcgatgcc tcccgcgctt acgccaaaga cggcgacacc
    13621 atcgccgggc tgtctgtgga tggcgttgcc tggccggacc tgcgcctgat gatgatcgat
    13681 tgcgaggaat tgacccgaaa cagccacgcc gtggcgcgcc atcccgaaat tgcctcctgg
    13741 tcttcggccc ggtatgccgc gagcggctac gcgctggccg gcgaggcggc acggcgcgca
    13801 ttgcaggatc tcatcgacag caaaggcatc cgcgccatcg aattgaaccc tcaccggggg
    13861 gcttcgggcg cctacgacga ccgtattgac gcctacggcc gttatgtcgg cctggtctat
    13921 ggcggcggcg aatgtttcaa cgcggccatg gtggaactgg gccattccga ggtctatcag
    13981 tacaaagacg gccggtatca tgcgcccgaa atgcggctca aggagtattt ttcctacacc
    14041 cgcgccagca gcgattccat cgaagcggtt ccggtcacgt tgaacgcatt tgaagcggat
    14101 cacggcctct ttgatctgtt gacggcgctg gcctatgcgg gcggatgcgt ctggaacctc
    14161 agccctcacg acgccgtgca ttttcgcccg gccggcatct gcgatcacgt tctcgcctac
    14221 gacccgctca ccataggcgt ccagcttgcc tcggacggcg ccagtatcgt caatgccctc
    14281 tattttgaag gcaatcccat cctgggaccg gtcaaaaaga cgtatattcg cggggagagt
    14341 gcggacacgt tcggatttcg cgcgcggcgg ttccggcatt ttggcattgc cagcgaaggc
    14401 gacgccgatc ggcttgccaa cggcctgctt catgacctgg cctaccccga ggccgcgggc
    14461 ttcctcacgc tctatcacgg cgccgcagcg ttccaggcgg gcgacctgct cgaactgcgc
    14521 ggagcgccgc cgcgacggct tgaccttccg cttcccgggg aatggggcgg ccagttcgcc
    14581 gggcgcctgg tccggcgcgt caaccgggtg acccaccggt tttcagggcg cacggtaact
    14641 acgaccctcg gatgcacctc tccgttgcgg tctgtggcga atcccctcta ccacatgatg
    14701 cagcaccagg ggccggaaca gtctctgtat cagtttcgcc tggatgaccc ggcggtgggc
    14761 ctggattcgg gctatcacct ggaataacta ccggacgccg cggcgcgcgc ggttttccgt
    14821 gggagatacc atggccattc gtggaaaaag cttcatcaag acgtcctggg cctttcagga
    14881 gcggcccgtg gcctcggcca aactcaacac ctgggacgat cgcatcgagg ccgctctcga
    14941 actcctcatg aacctcatca gcctcgcctg gggcggcggc aacggcatcc ttcgaaacgc
    15001 tgccgacggc gatctcggca tctcggccat ttctccgccg gccctggcgg tacaggtcgc
    15061 gccgggatac gccttcatcg agcggtttcc gtttcatttg aaaactgcga cggtcctcgg
    15121 cggcattgcc gcgccgctgc atcatccgcg catcgacctg gtgcaagccc ggctcgatac
    15181 ctgggatgtc accatcacgc cgggggttga agctgtgtcg ccatcgccgc ccggacccgg
    15241 cgccgcgtgc cttgccctgg cccacctcca tctgcgcccc ggcatgaccg ccgtcgtcaa
    15301 cacggatgac ggcctcaatg gatacatcag cgatgtccgc acttacgtat aaccgccgaa
    15361 acgatgtccg cctgttgacc aatctcgacg gcagagaggt ttcccatttt cgtctgcgtg
    15421 agttcgaaaa tgccgcgggg ttggccatgg tgcactccac gctgctcgaa tccctcgagc
    15481 gggtccgccg ggatctctgc gagatggccg gcgagaccgt gtggatcctc gtcaccagcg
    15541 cggtgcgtac cgaggaggac ctgcgccgcc tggccgccct ttacggatgg acggaccagg
    15601 gcggcgccgt gtcccgcaat tcccggcatc tcgcaaaatt cggcggcatc gcggccgaca
    15661 tcgtggccgt gctcgaacga aaccgcgcac gtgtccctca gaaaacgctt ggagccgtgt
    15721 gccgccgcta cttcgactgg gtaaaagacg attacaacga cgggcacgtc cacgcggaca
    15781 accgccatcg cgcggcgccg ccgacgtgac cgggaggatg cctaccgtga ccttcaacga
    15841 cgcgatccat tctgaccagg gactcatgct gctcggtgtc gtactgggcg gactctggtc
    15901 tcttttcaaa tcgactgatc tgtacgcgcg tgcccgccgc ctccggtgcg aacgcgccct
    15961 tcttgccctc gaaacgggtg tcatgaacac ctacgaaact tacgtgcgcg agatcaaggc
    16021 ctcgcgtgaa gacggcaaac tgacctccgc ggaacgaaaa gaggcccgcc gccgcgccaa
    16081 ggaatacgcc atcgagctag cgcgggtcga gggcctggat atcgcccgag aactcgggca
    16141 cagctacctc gacgcctggc tggaacgcct cgtacggcag gtcaaatcct gaactctccc
    16201 gcatccctcg cccctccctg cccccgcggg cgccccacca ccggggcgcc cgtcctcttg
    16261 caacgtctcc tcgatgctgc gaacctctcg ccggagcgcc ggacgggcgc agtggtgttc
    16321 gttgccgaaa tcgggtatgg tgtccgcaaa accaaaggcg gacgttcatg ctgcgagtcg
    16381 ctctggtagg cgcgggcaat ttcgccgcga gacactacgc cgcgttgcgc ggtttccccg
    16441 agcgcgtgga ggttgcactg gtcgcgcgca accagtgcga cacccctttt gaacccgcgc
    16501 cgcagctgcc ggttcttggc ttcgatgccg tcctcgccga cccctctatc gacgcagtcg
    16561 tcatttcatc gcccaacggc ctgcactgtt cccaggccga ggctgccctt cacgcaggca
    16621 aacacgtatt ctgtgaaaag cccctcgccc tgtgcggcca cgccgcgcgt gctgtggccg
    16681 gcgctgccgc ccgcgccggg cgcgtcctga tggccggcca cctgacccgc catacgccgc
    16741 tgtatgccgc cgcagccgag attgtgcgct ctggcggcct gggtcaagtg cggacaattc
    16801 atgccgcccg gtggcagaca tccgacaagg gccgcgactg gcgcatgcag cccggccagg
    16861 gcggcggcgc cccattcgac ctgctgctcc acgattacga cctcctgcaa tggctgtgtg
    16921 ccgtgcccga gagcgtcgta gctacagggc acaagcaccc ctccggagcc taccagcggt
    16981 tgacggcctc cttcgcatgc ccggatggcg tcgcggccac ggtcgacggc gggttcgtgc
    17041 tctccccggg agagaccatg aaatcatcac tgcatgtcgc tggaacggaa ggcgtgctcg
    17101 acatacagac ccccggcgaa gaccccatcc tcctgcgcca ggaaggcggc gagcccgaac
    17161 cagtctccat cgacccggac cgcatgggcg tcgatgggtt gcgcaccgaa tttctcgaat
    17221 tttttgatgc cattgacgga caagcctgga accggttgcg gctcgacgat gcctgcaacg
    17281 ccgtggccat cgccgccgcc gcactcgccg ccgccgaaac caactgccgc gtcgacctga
    17341 ctaccgggat gtgaggaaat acgggtcgaa gcggaggcgg tcgagcgcgg tttcgatgag
    17401 acccatcgcg tcgatgaggc ggatctccgc attgccgacg cggtctttgc gcatcaggcc
    17461 ggcttcgaga agcagcggtt caagggcggg aaaattgcgt tcggggccgt cgtaccggtg
    17521 cagacggcag ggcgtctcga ttcgcacgcc gcactgatcg atgcacgatc catgggttac
    17581 acgcggctga agatgataat ccacgttggc gagttcttcg gcggtgtggc aggtggtgca
    17641 gcggctcaag tcgcaaccaa tcattacaat cgtcccaccg gcgagcgcca ggcgaaagta
    17701 cggcgaggtc actccgcacg gcgtgggaga cagatagtgg ccgcgggtca gctcggctgc
    17761 gcgccggcca agggctgcgc aggaatgtgt ggggtgtacg ctgcgcactg cgtcggggcg
    17821 ctgccgcagc gtctcaggaa tgcgccccac ccagcagggc gcggtgcgca aatcgatatg
    17881 aggcgggttt tcgggcgaca gttccgggcc gccggtcagc gtgggcgcca gcagcgtgcc
    17941 ttcggggccc agcgcatcga gcaggccgtc gagcacggcg tccgcgccgc ctgccacgcg
    18001 accgaagctg ctcagggaac tgtgtacaag gatggcctca ccggagcgaa ggcctgcctc
    18061 ccgaaatccg cgggcaatct ctgcccgcgt aatctcgtcc ccggccatgt acccgtctcc
    18121 ccgaaattag aacgcttctc cgcgcatgtg ggcgtcggcg cattcggctg tgtagtcttt
    18181 gtcgagcttc ggaaaaagca cctcgggctc ttgcaggggc gagcccgcgg ccaggccgtc
    18241 cctgcctgcg ttccagccgt ctgcaccgcc atcagacccg ccttccggca aggaaatgcc
    18301 gatcaccttc gcgagctgct gcgcgccatc aggcacgaac ggcgccatcg ccacgcataa
    18361 ggcgcgcacg acctggcagc agacgtataa ggtggtcgcg gtgcgcccca ggtcggtctt
    18421 ccgcgtcatc cagggttgtt tgccgtcgaa atagacgttg gccttgcggg ccagatcaac
    18481 aaaccgttca agcccctgcc ggaaccggaa gttttcgagc gcctcggcta ccccgtcgag
    18541 ctgctccggg atagccgcca gcatcgcccg gtcaccgtca tccaatgggc ccggctcagg
    18601 cacccgggac tcgaagttct tcacggtcat ggtcagcgaa cggtgaatga aattgcccac
    18661 gacattggag agctctccgt tgtaccggcc gtagaaatcg tcccagtcga acgccgcgtc
    18721 gcgcgattcg ggcatgatcg agcaaagata gtagcggatg gcgtcggtcg aaaagcgcga
    18781 cgtgaaccac cgcacgctga tcatgttgcc tttggatttc gagccttttt cggtcttgcc
    18841 ggtctcgcgg ttgaggatgt tgaggtattc attgcccacc acgttgtcgg cgaggatata
    18901 gtctccctgg cccatcagat aggtgggaaa catgaccgca tggaacggca cgttgtcttt
    18961 cccgatgaaa tggatcagcc ggctcgcggg gtctttccac caccgttccc agccatccgg
    19021 ctcgccccgg tcgaggtagt actgccgcgt gttcgtaacg tagccgatgg cgttgtcaaa
    19081 ccacacataa aaccgtttgc ccgccgtgtc g
//
LOCUS       DAOPBQ010000008        18426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_3119_length_18426_cov_3.993795, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000008 DAOPBQ010000000
VERSION     DAOPBQ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 18426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18426
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3119_length_18426_cov_3.993795"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..93)
                     /locus_tag="PKW60_00630"
     CDS             complement(<1..93)
                     /locus_tag="PKW60_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPJ97963.1"
                     /translation="MTSLITLTSDFGERDPYVAAMKGVLASLCPQ"
     gene            229..555
                     /locus_tag="PKW60_00635"
     CDS             229..555
                     /locus_tag="PKW60_00635"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HPJ97964.1"
                     /translation="MKYVKIANVSDFESKRFRVFTILAKKVGIFRGEDGALFATELVC
                     KHQNWDLSTGRIEGNLCTCPRHGWQYDLRTGECLNHDSAPLRRYGLRCDGDDIYITLR
                     PIDSES"
     gene            complement(601..1290)
                     /locus_tag="PKW60_00640"
     CDS             complement(601..1290)
                     /locus_tag="PKW60_00640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPJ97965.1"
                     /translation="MNSPEQEAAGSQRAALLAAAYVLLVLGLDALATQNMRFPVEWRL
                     LLKYQPGGFDVFKFAAWFALPLLCLLPRLDVHAFTFTRWRRADWYLLAGLAAMGALAV
                     LLIPLLPGVREMYRGTGAFLARRRWEASIWYISWTASWLPGWELLHRYVLLRSWDRWL
                     PRAGWLIVPLSEGVYHLQKAPLEALGMVALSIVLTLWARKRRNVLLPFLAHAIIELEL
                     LAFLLYASPGV"
     gene            complement(1303..1980)
                     /gene="nth"
                     /locus_tag="PKW60_00645"
     CDS             complement(1303..1980)
                     /gene="nth"
                     /locus_tag="PKW60_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPJ97966.1"
                     /translation="MAANKMTRPAKRERATEVLDRLMKAYPDARCSLDFKDPLQLLVA
                     TILAAQCTDARVNLITPSLFKRYRTAKDYATAADDELQKAIQSCGFFRQKARSIKQAS
                     QTIVERFGGTVPGTMEELLQLDGVGRKTANVLLGECFGAPGITVDTHCKRVSTRLGFT
                     KSANPDKIEQDLMGLLPEAQWTKFSHCLVFHGRAICQARAPYCSRCCVRDLCPFPDSR
                     EGRKIAR"
     gene            complement(1962..3668)
                     /gene="polX"
                     /locus_tag="PKW60_00650"
     CDS             complement(1962..3668)
                     /gene="polX"
                     /locus_tag="PKW60_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HPJ97967.1"
                     /translation="MTRKEIAAVLEEIAVLLELSGENPFKSRSYSNVARQILQSGDDI
                     NQLVKEKRLRELKGVGDALEQKITELVTTGQLQYHQELRAKFPETLFDLFQIPGLGAR
                     RIKLLHDELGIATLGELEYACEENRLASLKGFGPKMQQNVLDGLAFAKRHQGQHLVSA
                     GWEAANALLDWLREQTPVSRLEVAGSLRRRKEIVKDIDLLAAGGAPEQIMAGFVGAPG
                     VETVTGHGETKSSVVLASGIAVDLRVVSEAQFPYALAHFTGSKEHNVVMRQRAKDRKM
                     KLNEYGLFAGEERLVPCGDEAAIFAALGLPNIPPELREDRGEFDADTMPALIQRPDLL
                     GVMHCHSTYSDGRSSVEEMTSGAKARGYRYIGFADHSQSAVYAGGLAPDDVKRQHGEM
                     DRVMEKIEGIRILKGIESDILADGSLDYDEKTLKRFDFVIASVHSKLTMTEREATQRL
                     LAAIRNPHTTILGHPTGRLLLSREGYPLDLDAIFDACAEYRVAIEINANPHRLDLDWR
                     HLRKASERGVKLCIGPDAHSVDGLDDVEYGLGIARKGWLTKDHLLNCMEAEELLSWRQ
                     TR"
     gene            complement(3703..6066)
                     /gene="lon"
                     /locus_tag="PKW60_00655"
     CDS             complement(3703..6066)
                     /gene="lon"
                     /locus_tag="PKW60_00655"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPJ97968.1"
                     /translation="MTGDNSAPHVVLTDGTASTPELLPLLMLRKQVLFPMAALPIRLT
                     DPHEKRLIDEAVLGPKMVALLTLREQDGEAQGLDAAYDTGTTARILQLQRLPDDSLVV
                     LLQALSRFRPLGLTRREPYPFVRVQLLEDQISHPDKIPALATAIRQQMTRYAQLSPSM
                     PDEAAHVLDNIEDPGRLADIVAANLNIPIEEKQRLLSLIDPRERLERLVYLISNELQV
                     METSHKIQAEVKSSIDASQREFYLRQQLKAIQDELGMGGRDHPEIDTYREKIEALQIN
                     DAIRKEALRELDRLGQMNESSAEYHVILTYLDWFTDLPWNNETRDQLDIGRAEEILDE
                     DHYGLAKVKKRILEYLAVRKLQPEAPGPILCFAGPPGVGKTSLGQSIARALGRTFVRM
                     SLGGLHDEAEIRGHRRTYVGAMPGRILQGIRKAGSNNPVFMLDEMDKAGADFRGDPSS
                     ALLEVLDPAQNNSFNDNYLNVPFDLSRVMFIGTANVLDTIPWALRDRMEIIEIPGYIL
                     EEKVHIARNYLVPRQMTANGIPPKRVTITAAALRRIVESYTREAGVRNLEREIGAVLR
                     GCATAIAKGRRKPIRIDAKDLGQYLGPERFYREVLDRTRLPGVATGLAWTPTGGDILF
                     VEATRMPGKGGLTLTGQMGDVMKESAQAVLSYVRANAESLGVSPEDFRDFDIHIHVPA
                     GAVPKDGPSAGVTMLTAVASLLTGKKVKPNLAMTGEITLRGLVLPVGGIKEKVLAAIR
                     AGVKHIILPERCAKDLDEVPEKARKKVTFYFVSEMREVLHLALGLKL"
     gene            complement(6063..6440)
                     /locus_tag="PKW60_00660"
     CDS             complement(6063..6440)
                     /locus_tag="PKW60_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPJ97969.1"
                     /translation="MPRYPNIYFQMYAMTPRRQLESFSPTDWEPPLDIYETDTKFVII
                     AELPGVKKDQVHVRVEQNILTIEGERPKRVPLRTVHVHQMEIPYGRFARRIQLPAETD
                     VERIEAKFREGYLRIEVPRSMTR"
     gene            6602..7309
                     /locus_tag="PKW60_00665"
     CDS             6602..7309
                     /locus_tag="PKW60_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPJ97970.1"
                     /translation="MSADSVVIIPTYNEAQNIQRLISAIQDACSALDIVIVDDASPDG
                     TGELAASMEGVHVIRRPGKLGLGTAYVAGFCYALGRGYQRIAGMDADFSHDPSVLPAL
                     FALLDTADVGIGSRYVPGGGTRNWGLHRQILSRTANIFARFMLGLPIHDVTSGYRCYR
                     REVLERIDLDTLASHGYSFLVELLYRSVQAGFSVAETPIIFEDRARGTSKMSLREIVG
                     GIKNLFRLRRTEMKTRS"
     gene            7564..9069
                     /locus_tag="PKW60_00670"
     CDS             7564..9069
                     /locus_tag="PKW60_00670"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPJ97971.1"
                     /translation="MAERIAIFDTTLRDGEQSPGASMNAHEKIQMALQLERLGVDVIE
                     AGFPIASQQEFESVQQIAATVKRPGVAALARAVDKDIERAAAALEKAAFPTLHTFIAT
                     SDIHLEHKLRMTREQVLEQAGNGVRLAKSLIGRVEFSAEDATRSELDYLVAITKVAIE
                     AGASVINLPDTVGYTTPGEIEEMFRYVIANTPGSDTVVFSSHNHNDLGLAVANALAAV
                     RGGARQIECTVNGIGERAGNTSLEEVVMTIKTRQNIYPFETGIVTEELTNASRQLSNV
                     TGLPVPYNKPIVGRNAFAHEAGIHQHGVIASRLTYEIMTPESVGRTRSELVLGKHSGK
                     HGLAKRCEELGYVLNKEELQRLYDRFIELTDRKKEVFEDDLRVLIASLRHENFETYRL
                     LHVRTSGGDPTMALVKLQRGKDTMFETATGDGPVHAACMAIERISGVSGRLSEFQVRA
                     ATPGKDALGDAHVTVEFEGKAYYGTGASTDIIEAAVMAYLNALNKYFAMKT"
     gene            9230..10537
                     /locus_tag="PKW60_00675"
     CDS             9230..10537
                     /locus_tag="PKW60_00675"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HPJ97972.1"
                     /translation="MKFGNTIEEYVHSNEPQTRSGMLLLESARRLRIKPDDVAEIIKP
                     YTVHIQRLPVPLMGKVINLTSGIVLHNRARGPYKGGIRIAKDVDIWETTELARLMTLK
                     TALADMDLGGGKSGISVDLRALYTAMCEEKRFVGDFREFARIAKADIMVEFTSHFGRL
                     FSRNHYIPAPDMGSGAAEMARIYNATLDPASVTGKPDGVEGWLPGRTEATGYGIWYAI
                     LKDMERRGLAPDQCTAAIQGFGNVGMHAATALQGSGVRIVGIADITGGINDPAGLDVQ
                     RVAVHYGRHGSLTGYKARSLTSQRLFRLPVDYLIPAATGHVIHASNAGQIKARVIVEG
                     ANMPVTYDAMKTLAEKGVDLIPDIYANAGGVIASHLEFRQALSGQRFTRAMTLAHIRE
                     RFDATYEAMLPFMKKGRSMTEASIDVALQRVHQAMTERELLAR"
     gene            10546..11034
                     /locus_tag="PKW60_00680"
     CDS             10546..11034
                     /locus_tag="PKW60_00680"
                     /inference="COORDINATES: protein motif:HMM:NF017434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HPJ97973.1"
                     /translation="MREKAAGHPREQAAKTIIGWSEYVALPEWGISRLPAKIDTGART
                     SSLHVDNLQIVDAHHVSFEVVLSRKHSNRRIHVVTRIAKWGRVRSSTGHYHTRCFVHT
                     RARIGAIEKNIELSLASREKMLYRMLLGRKALEKDFLVDVSRRRVLGKPEKPRQKRSV
                     PS"
     gene            11031..12218
                     /gene="rimK"
                     /locus_tag="PKW60_00685"
     CDS             11031..12218
                     /gene="rimK"
                     /locus_tag="PKW60_00685"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HPJ97974.1"
                     /translation="MKIGLLSRQPRSYSTRRLREAAKRRGHSVRVLDTLRFAISLEKQ
                     EPDLYYRGRKLPALDAVIPRIGASITFYGLAVLRQFEQMGIYVPNDSAGISRSRDKLC
                     ATQLFSRYSISIPATEFVRDSSDILPAIRRVGGVPVIIKVIEGTQGVGVILAETEKAA
                     EAIIQTLHGAKQNVLIQKFVKESHGSDLRAFVVGGRVVAAMRRVAKPGEYRSNVHRGA
                     SVEPVTLTPEFTHTALRAAQIVGLGIAGVDMLESAEGPQVMEVNSSPGLEGIEKATGI
                     DVASAVIEFVEEQALFPDADLRQRLRLTGGVGIAEVSVHGMPELEGKPLRETPLASRN
                     IYVLNIAHHDTVVPHPKADDVIQTGDVLLCYGDLRELRSMMPPRRRRKRPRKRSSWEK
                     SKG"
     gene            12215..13360
                     /locus_tag="PKW60_00690"
     CDS             12215..13360
                     /locus_tag="PKW60_00690"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HPJ97975.1"
                     /translation="MSARNGLNGRTARATRPFEERETLVIAGQSIPLGETRELQLPFD
                     ETYLGEPVSVPIYVMRAPQPGPRVFLTGTLHGDELNGMGIIRELLYGNPLQLVRGSLI
                     LIPVINIHGLERHARNMPDRRDPNRCFPGSARGSQTSRMAHAIFTEIVKQCDYGIDFH
                     SAAIRRVNYPNVRAYTRNLKTRMLAEAFGCELIINNQGPQGSLRRSAVQAGVPCIILE
                     AGEVWKIEPTVVEIGVRGVLNVLRTVGMLQGEPRKPPFQLIVTKTSWVRAPTGGILDF
                     HARPGDLVRRNECLATISSVFGREHSALLAPVDGIVLGMTTMPAVKPGGPVYHLAVLS
                     KRNVRVALHHLMNGERRGMFGRVRRELSSAMAFRERESRYGDAKSQP"
     gene            13512..14522
                     /locus_tag="PKW60_00695"
     CDS             13512..14522
                     /locus_tag="PKW60_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ97976.1"
                     /translation="MDTLGMGIVGCGWVAEEYIKAAQCDGRVEVRALVSRTPGKPETY
                     RQRYNLDATIGNDYEDLMQRDDIDIVVVSTPHDLHTGPVVAAAEAGKHVIIEKPVALT
                     MDDVRKQQAAVQKTGVKTIVSFVLTRNPLLISIDALIRDGALGNVFLVEVDYLHRIWC
                     GSEKWLGRKDKAGSSFLAGGCHAVSAMQWFARSEAVEVCAYSVQTENPNDYPGTTTAV
                     VKFADGTLGRTTSCFDAQMPYVFHIGVYGTEGSVRNDKVFAPKIFPGQNAFMTVPCVL
                     PDSGDVAHHPFQGEVSHFIDCILEDKKPFPNLDDAAKTMAICIAADISSVEGRPVKTT
                     EV"
     gene            14633..16153
                     /locus_tag="PKW60_00700"
     CDS             14633..16153
                     /locus_tag="PKW60_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97977.1"
                     /translation="MTERTQNGLNRREFFVHTGRGAMVGGILGTTGPARTVLAQTQES
                     SQPSAAASAGSLQVGWAAASITPDQPVQLHGQFHERVSKYVLDPCMATALALERDGEQ
                     ALMVSCDIVNVQRAVTEEIRSRVAQQLPGFDPSKFFINATHTHTGPTMSVGLFKDPDP
                     GVITPQEYVEFFTQHVTEAAVAAWNARRPGAVSRASGQAAIGFNRVAKYKDGSSQMYG
                     KSDRPDFQGLEGGDDHGLEILFFYDDKQVMTGTLINVACPSQVVEGQYFVSADFWGPA
                     REQLRALYGEQLYVYAMVSAAGDQSPRDLVRRGRNEPDMRDIAGQQEMARRLVNAVQI
                     AHGTTKTPPVNDLEFRHHVEELELPARKVTDDEVTAAREEIARLTKDGPPQPTTSQAG
                     TVRRMNRLIERHSAQGDAPAYSMDLHVIRLGDIAIATNPFELYLEYGSQMKARSRAEQ
                     TFIAQLTSDRGGYLPTRAALPGKAYGSRVTENTVGPEGGELLVERTVEIINSMWEA"
     gene            16217..17047
                     /locus_tag="PKW60_00705"
     CDS             16217..17047
                     /locus_tag="PKW60_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPJ97978.1"
                     /translation="MADIQVGLNTEAFRHADKPLEYALEAIRKQGYRYCELNMLNGRD
                     LLCEAGYYAAVSMERDPLEVKAIVDSFGLKVSAVSAHVPMAQPEISIPFLTRAIQFAA
                     GLGATCVCTDEGMVPGWMNKTQAFNLIYYTLRRVLPVAERHKIHIALEPHQVYSVKLK
                     TYLEILNLIDSPYMSCNPDTGNIFMAGQDPYEFLEAIAKRVVHVHAKDIGGVVMSDRG
                     KVTGVPSGCACGEGVLDWQRIVSILRKAKFKGVLSVECSSEEQGRASIKYLKKVLAQK
                     "
     gene            17203..17406
                     /gene="rpmE"
                     /locus_tag="PKW60_00710"
     CDS             17203..17406
                     /gene="rpmE"
                     /locus_tag="PKW60_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPJ97979.1"
                     /translation="MKKGIHPDYKAARVTCACGNTFETKSTKGDINVEICSECHPFYT
                     GKQKFVDSEGRVDRFNRKYGRTS"
     gene            17428..>18426
                     /gene="prfA"
                     /locus_tag="PKW60_00715"
     CDS             17428..>18426
                     /gene="prfA"
                     /locus_tag="PKW60_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPJ97980.1"
                     /translation="MREKLRDVVAEYDRLSRDMASQEVASDPDRYMKCAKAQKHIEPL
                     VDCFKKYENEENRLEQAEAILYEESDPELRQMAEDEKRLLTEALEARELELKLLLVPG
                     DPNDEKNTIMEIRAGTGGEEAALFAADLFRMYSRYAELKGWKVEILNSHTTGMGGFKE
                     ISFKVAGDRVYSLLKFEGGVHRVQRVPETEAQGRVHTSAVTVAVLPEAEAVDIAIDEN
                     DLQIDVYRSSGPGGQSVNTTDSAVRVTYKPTGLVVTCQDEKSQHKNKAKALMVLRARL
                     LAQKQEEEAAERSANRKSQVGSGDRSERIRTYNFPQNRLTDHRIKLTVHRLETVMEG"
BASE COUNT         3750 a         5840 c         5366 g         3470 t
ORIGIN      
        1 ctgtggacag agcgaagcga gcacgccttt catggcggcg acgtaagggt cccgttcgcc
       61 gaagtcactc gttaaggtaa tcaaggatgt cataggcgga cacaccggcc cgcgcgggcc
      121 atttggtgtt gtatttcttc ccagttctcc gtatggtcgt gcacaaatca taacaggaca
      181 atcatcggct atgaaagaag aaggggaggg gcggctgccg gcagcgacat gaaatacgtc
      241 aaaattgcca acgtcagcga ttttgaaagc aaacggtttc gggtttttac catcctggcg
      301 aagaaagtag ggattttcag gggagaggac ggcgctttgt ttgccacgga actcgtgtgc
      361 aaacatcaga actgggatct ctccaccggc cgcatcgaag gcaacctctg cacctgtccc
      421 cgccacggct ggcaatatga tctgcgcacc ggggaatgtc tcaaccacga ttccgcaccg
      481 ctccggcgct atgggcttcg atgcgacgga gacgacattt acatcaccct tcgcccaatc
      541 gacagcgaat cgtaacgcga tgacaatctt tacggtgccg gcccctggag gtttgccagt
      601 ctaaacaccc ggcgacgcgt acaacaggaa ggcgagcaat tccaactcga tgatcgcgtg
      661 ggcaagaaac ggcaggagga cgttccggcg tttacgcgcc cagagggtca gcacgatcga
      721 cagagcgacc atgcccaggg cttccagagg ggctttctgc agatgataca ccccttccga
      781 caggggcacg atgagccatc ccgcccgggg aagccatcga tcccaggagc gcagcagcac
      841 gtaccggtgc agcagttccc acccgggcag ccaggaggcc gtccagctga tataccagat
      901 actggcttcc catcgccgcc gggcgaggaa ggcgccggtt ccgcggtaca tctcgcgaac
      961 cccgggtaac agggggatga gcagcacagc gagcgcgccc attgccgcca gaccggcaag
     1021 caggtaccag tctgcccgcc gccaccgcgt gaaggtaaag gcatgaacgt cgaggcgggg
     1081 gagaaggcac aggagcggca atgcaaacca ggccgcgaat ttgaacacgt caaagccgcc
     1141 gggctgatac ttcagcagca ggcgccattc aacagggaat cgcatatttt gcgtggccag
     1201 tgcgtccagc ccgagcacga ggagcacata ggcggcagca agcagggctg cgcgctgtga
     1261 acctgctgcc tcctgttcgg gggaattcat gccgtgccct gcctatcggg caatctttct
     1321 gccctcccgg gagtcgggga acgggcagag atcgcgaaca cagcaccggc tgcaatacgg
     1381 cgcgcgcgcc tgacagatcg cacggccatg aaacaccagg caatgggaaa acttcgtcca
     1441 ttgcgcttcg ggcaggagcc ccatgaggtc ctgttcgatc ttgtccgggt tggcgctctt
     1501 tgtaaaaccc agccgggtgc ttacccgctt gcagtgggtg tccacggtga taccgggcgc
     1561 tccgaagcat tctcccagca gcacattggc cgtttttcgt cccactccgt ccagctggag
     1621 caattcctcc atcgtccccg gcaccgtgcc accgaatcgc tcgacaatag tttggctggc
     1681 ctgttttatt gacctggcct tctgacggaa aaaaccgcag ctctggatgg ctttctgcaa
     1741 ctcgtcatcc gccgccgtgg catagtcttt cgcggttctg taccgcttga agagactggg
     1801 cgtgatcagg ttgacccggg catcggtaca ctgcgccgcc aggattgtcg caacgagcag
     1861 ttggagcgga tccttaaaat cgagcgagca gcgcgcatcg gggtaggctt tcatcagccg
     1921 gtccaacacc tcggtcgcac gctcccgctt tgccggccgt gtcatcttgt ttgccgccat
     1981 gagagcaatt cctctgcctc catgcaattg agcaggtgat ccttcgtcag ccagcctttc
     2041 cgggcgatgc ccagaccata ttcgacatcg tcgaggccgt cgacgctgtg cgcgtccggt
     2101 ccgatacaca gctttacacc gcgctcgctg gctttgcgca ggtgccgcca gtccaggtcc
     2161 agccggtggg ggttggcatt gatctcaatg gcgacccggt actcggcaca tgcgtcaaag
     2221 atggcgtcca gatcaagggg atacccttcg cgcgacagga gcaaccgtcc agtggggtgg
     2281 cccagaatgg tcgtgtgggg attccggatt gcggccagga gccgctgggt cgcctctctc
     2341 tccgtcatgg tgagcttgct gtgtaccgag gcgatgacga aatcgaaccg tttcagggtc
     2401 ttctcatcgt aatcgagtga gccatcggcc aggatatcgg attcaatgcc tttgagaatc
     2461 cggattcctt cgatcttctc catcacccgg tccatttcac cgtgctgccg cttcacgtcg
     2521 tccggcgcca gtcccccggc ataaaccgcg gactgactgt ggtccgcaaa gccgatgtaa
     2581 cggtagccgc gtgctttggc gcctgaggtc atttcctcaa cgctgctgcg gccgtcgctg
     2641 taggtggagt ggcagtgcat tacgccgagg agatccgggc gctggatcag cgccggcatc
     2701 gtatcggcgt caaattcgcc gcggtcttcc cggagctcgg gcgggatatt gggaagtccc
     2761 aaggccgcaa atatggccgc ctcgtctccg cagggcacga gacgttcctc accggcaaac
     2821 aacccgtatt cgttcagctt catcttgcga tcttttgcgc gctgccgcat cacgacgttg
     2881 tgctctttgc ttccggtgaa atgagcgagc gcatacggga actgcgcttc ggaaacaacc
     2941 cgaagatcca cagcgatgcc cgaggcaagc accacgctgg acttcgtctc cccgtggccg
     3001 gtcacggtct cgacacccgg tgcgcccaca aagcccgcca tgatctgttc tggcgcgcct
     3061 cccgccgcga gaaggtctat atctttgacg atctccttac ggcgccgcag acttcccgcg
     3121 acctcgagcc gcgacacggg agtctgttcc cgcagccagt cgagaagggc gtttgcggcc
     3181 tcccagcccg cgctgacgag gtgctgcccc tggtggcgct tcgcgaacgc gagcccgtcc
     3241 agcacattct gctgcatctt cgggccgaat cctttgagcg aggcgaggcg attctcctcg
     3301 cacgcatatt ccaattcgcc cagggtggca atgcccagct cgtcatgaag cagtttgatc
     3361 cgcctggcgc cgaggcctgg tatttgaaac aggtcgaaca aggtctcggg aaatttggcg
     3421 cgcagttcct gatggtactg cagctggcct gtagtcacca gctccgtaat tttctgctcg
     3481 agcgcatctc cgacgccttt cagttcccgc agccgtttct ctttcacgag ctggttgata
     3541 tcgtcgccgg attgcaaaat ctgccgcgcc acgttgctgt aagagcgcga cttgaacgga
     3601 ttttcgccgg ataattccag cagaacggcg atttcctcca ggacggccgc gatctctttg
     3661 cgggtcatgt gaacgctcct tcgtgcagcc agggcccgcg catcacagtt tcaggcccag
     3721 cgccagatgc aatacctccc gcatttccga gacaaaataa aacgtgacct tcttgcgcgc
     3781 cttttccggc acttcatcga gatccttcgc acaacgttcg gggaggatga tgtgcttcac
     3841 gcctgcccgt atggcagcga gcactttttc cttaataccc ccaaccggca acaccaggcc
     3901 gcgcagggtg atttcgcccg tcatggccag gttgggcttc acttttttcc ccgtgagcag
     3961 cgacgccacg gcggtcagca tcgtcacacc cgcgctgggc ccgtctttgg gaacggctcc
     4021 cgccggcacg tggatatgga tatcgaagtc ccggaagtcc tccggggaga caccaagcga
     4081 ttccgcgttg gcccgcacgt agctcaagac ggcctgagcg gattccttca tcacgtcgcc
     4141 catctgccca gtgagcgtca atccgccttt gcccggcatg cgcgtagcct cgacgaaaag
     4201 aatgtctccg ccggtggggg tccaggccag gccggtcgcc accccgggga ggcgcgtccg
     4261 gtcgaggact tcgcggtaga accgctccgg gccgaggtac tgtccgagat cttttgcgtc
     4321 tatccggatg ggttttcggc gtcctttggc gatggcggtg gcgcatcccc gcaggacggc
     4381 gccgatctca cgttcgagat tgcgcacgcc ggcttcgcgg gtgtaagact caacaatgcg
     4441 ccgcagggct gccgcggtga tggtgacccg tttcggggga atgccgttag cggtcatctg
     4501 gcgcggcacc aggtagttcc gggcgatatg gaccttctcc tcgaggatgt accccggaat
     4561 ctcgatgatc tccatccggt cacgcagggc ccagggaatc gtatccagca cattcgccgt
     4621 gccgatgaac atcacccgtg acaggtcgaa gggcacgtta agataattgt cattgaacga
     4681 attgttctgc gccgggtcca gcacttccag cagggccgag gagggatcgc cccggaaatc
     4741 cgcgccggcc ttgtccattt catccagcat gaataccggg ttattggaac cggccttgcg
     4801 gataccctga aggatgcggc ccggcatggc cccaacatag gtgcgccgat gaccccggat
     4861 ctcggcttcg tcgtgcaggc cgcccagcga catgcgcacg aacgtgcgcc caagcgcccg
     4921 ggcgatggac tggccgagcg acgtctttcc aactccgggc ggaccggcaa aacagaggat
     4981 gggcccgggc gcctcgggct ggagtttccg cacggcaagg tattcgagga tacgcttctt
     5041 gaccttggcc agaccgtagt ggtcttcgtc caaaatttcc tctgcgcggc cgatatcgag
     5101 ctggtctctg gtctcgttgt tccagggcag atccgtgaac cagtcgagat aggtgagaat
     5161 gacgtggtat tccgcgctgg attcattcat ctgacccagg cggtccagct cgcgcaaggc
     5221 ttccttacgg atcgcatcgt tgatttgaag ggcctcaatt ttctcgcggt atgtgtcgat
     5281 ttccggatgg tccctgccgc ccatccccaa ttcgtcctga atcgccttga gctgctggcg
     5341 gagatagaat tcccgctggc tggcgtcgat cgagctcttg acctccgcct ggatcttgtg
     5401 cgaggtctcc atcacctgca gctcgttgct gatcagatac accagacgct caagccgttc
     5461 ccgcggatca atgagcgaca acagcctttg cttttcctca atcgggatgt tgagattcgc
     5521 cgccacgatg tccgccaggc ggccgggatc ttcgatattg tccagcacat gcgcggcctc
     5581 gtccggcatg gacggcgaga gctgggcgta gcgcgtcatt tgctggcgaa tggccgtggc
     5641 cagggcaggg attttatccg ggtgactgat ctggtcttcg agaagctgca cccgaacaaa
     5701 cgggtacggt tcccgccgtg tcaggcccag agggcgaaac cggctcaacg cctgaaggag
     5761 gacgaccagg ctgtcgtcgg gcaggcgctg cagctgcagt atccgggccg tcgtgcccgt
     5821 gtcgtaggcg gcatccagcc cttgagcctc tccgtcctgt tcccgcaacg tcaggagagc
     5881 aaccattttg gggcccagca cggcctcgtc tatcaggcgt ttctcgtgcg ggtcagtcag
     5941 ccggatcggc aacgcggcca ttggaaacaa gacctgcttg cgaagcatca gcagcggcag
     6001 gagttcggga gtcgaggctg tgccgtccgt gaggaccacg tggggggcgc tgttgtctcc
     6061 ggtcatcgcg tcatgctcct tggcacttca atgcgcaaat atccttcccg gaatttcgcc
     6121 tcaatgcgtt ccacatctgt ttccgcgggg agctgaatac gccgggcaaa ccggccgtag
     6181 ggaatttcca tctgatggac atgtaccgtg cgcaggggca ctcgtttcgg ccgctctccc
     6241 tcgatcgtca gaatattctg ttcgacgcgg acatgcacct gatctttctt caccccgggg
     6301 agttcggcaa taatcacgaa cttcgtatcg gtttcataga tgtccagagg aggttcccag
     6361 tcggtgggag aaaacgactc cagctgtcta cgtggggtca tcgcatacat ctggaagtag
     6421 atgtttgggt accggggcat tgctcgctcc ttcaggattt ctacgccgca acccgtgccg
     6481 gaaattctgc tggtgtgagt atatcggccg tgttttcatt gaagcaagag agctgcccgc
     6541 ttataattgc gcctcagcgt ctgagccgcc cgggcgcgcc tgcaggaaag gcgagtagac
     6601 catgtctgcc gattcggttg tcatcattcc cacatataac gaagcacaga acatccagcg
     6661 cctgatcagc gccattcagg atgcctgcag cgccctcgac atcgtgatcg tcgacgacgc
     6721 ctccccggat gggaccggtg aactcgccgc atccatggag ggcgtccacg taatccgccg
     6781 cccgggcaag ctggggctgg gcaccgccta tgtcgccgga ttttgctatg cgcttggccg
     6841 tggctatcag cgcatcgccg gtatggacgc tgatttcagc catgatccga gcgtgctccc
     6901 ggctcttttc gcccttctgg acacggccga tgtcgggatc ggatcgcggt atgttccggg
     6961 aggaggaacg cggaattggg gcctgcaccg ccagattttg agccgtacgg caaacatctt
     7021 cgcgcggttc atgttgggcc tgcccatcca tgatgtgacc tccggatacc gctgttaccg
     7081 ccgggaagtg ctggaacgca ttgatctcga tacactggct tcccatgggt attccttcct
     7141 ggtagaactc ctgtaccgct ccgttcaggc gggtttctcc gtagccgaga cgccgatcat
     7201 tttcgaagat cgcgcccgcg gcacgtcaaa aatgagtctc cgcgagattg tgggcggcat
     7261 caaaaatctg ttccgcctgc gccgcacgga gatgaaaacc cggagctgac cccgtcatct
     7321 gcggagcaaa tcagtgaaac ccacaacaga tgcgccatgt acagccgggc ggcaggattg
     7381 ccggacgctc tccctggcgc gccgaattgg gcggcgttgc cgcgtgcctg atgattaaaa
     7441 attgacacac tttgaagcgt tttgatactc tttcgcccct gcatgtttcg aacctcaatg
     7501 cgcatatcga cggaatagct gaggctcagc gcatcttgaa atggccggat tggaggacat
     7561 ccgatggcgg aacgaatcgc gattttcgac acaaccctgc gcgacgggga gcaatcgccc
     7621 ggcgcgagca tgaacgccca tgagaagatc cagatggcgt tgcagctcga acgcctcggt
     7681 gtcgacgtga tcgaagcggg tttccccatt gcgtcccagc aggagtttga aagcgtccag
     7741 caaatcgccg caaccgtgaa acgacccggc gtggccgcgc tggcacgggc cgtagacaaa
     7801 gatatcgagc gcgccgcggc cgctttggaa aaggcggcat ttccaacctt gcataccttt
     7861 atcgcgacgt ccgatatcca cctcgagcac aagctgcgca tgacgcgtga acaggtcctc
     7921 gagcaggccg gcaacggagt gcgcctggcg aagagcctca tcggccgcgt cgaattctcg
     7981 gcggaagacg ctacccgctc cgaactggac tacctggtcg cgattacgaa ggtcgccatc
     8041 gaggcaggcg cgtccgtgat caatcttccc gataccgttg ggtacaccac gcccggcgag
     8101 atcgaggaga tgttccgcta tgtcatcgcc aacaccccgg gctcggacac cgtggtattt
     8161 tccagccaca accacaatga tctcggcctg gccgtggcga acgccctggc ggcagtacgc
     8221 ggcggggcgc ggcaaatcga atgcacggtc aacggcatcg gcgagcgcgc cggaaacacc
     8281 tcgctcgaag aagtcgtcat gacgattaag acacgccaga acatctatcc gtttgagacc
     8341 ggcattgtca ccgaggagct cacaaacgcc agccgccagt tgagcaatgt cacggggttg
     8401 cccgtcccct acaacaagcc catcgtgggg cgcaacgcgt tcgcgcacga agccggtatc
     8461 catcaacacg gcgtgatcgc aagccggctc acgtacgaga ttatgactcc cgaatcagtc
     8521 gggcgcaccc ggagcgaact cgtcctggga aagcactccg gcaagcacgg cctggctaaa
     8581 cgctgcgagg aactgggcta cgttctcaac aaagaagagt tgcagcggct ttatgaccgc
     8641 ttcatcgagc tcaccgaccg gaagaaggaa gtgtttgagg acgatctgcg cgtcctcatc
     8701 gcttcgctga ggcatgagaa cttcgaaacc taccgcctgc tccacgtgcg tacctctggc
     8761 ggcgatccca ccatggcgct cgtgaaactc cagcgcggga aggacaccat gttcgaaacc
     8821 gcgaccggcg acgggcccgt tcatgccgct tgcatggcga tcgagcgcat cagcggtgtg
     8881 agcgggcggc tctccgagtt tcaggttcga gccgctactc cgggcaagga tgccctgggc
     8941 gacgcccatg tcacggtcga atttgaggga aaagcctatt acggcaccgg tgccagcacc
     9001 gatattattg aagccgccgt gatggcgtac ctcaacgccc tcaacaagta tttcgcaatg
     9061 aagacatagt ggtatctggc ggattcccgg cttcccgagc atgcccgcac tcgggggcct
     9121 tatccgtacc ggcgtcttta cgccggtacg gcaagacgag atgcaagtgg acagcggctg
     9181 cgagcccgct gaatacgggg acttccttcc ccgataagga acgcgacgca tgaagttcgg
     9241 caacaccatc gaagaatatg tgcacagcaa tgagcctcag acccgatccg gcatgcttct
     9301 cctcgaaagc gcgcggcgtc ttcgaattaa acctgacgac gttgccgaga ttatcaagcc
     9361 gtacaccgtt catatccaac gtcttcccgt gcctctcatg ggcaaagtga tcaacctcac
     9421 gtcggggatc gtcctgcaca accgcgcccg gggaccgtac aagggcggta tccggattgc
     9481 caaggatgtc gacatatggg aaaccaccga gcttgcccgt ctcatgaccc tgaagaccgc
     9541 gctggcggat atggatcttg gcggcggcaa atccgggatc agcgtcgatc tccgtgctct
     9601 gtacaccgcc atgtgcgagg aaaaacggtt tgtcggcgat ttccgtgagt tcgcccgcat
     9661 cgccaaagcc gacatcatgg tcgagttcac cagccacttc ggcaggctgt tctcgcgcaa
     9721 ccactatatt ccagcgcccg acatgggtag cggggctgcg gaaatggcgc gcatctacaa
     9781 cgcaaccctc gatccggcct ccgtcaccgg aaaacccgac ggtgtcgaag gctggctccc
     9841 cggccgtacc gaggccaccg gctacggcat ctggtacgcc atcctcaaag acatggagcg
     9901 ccgcggactg gcccccgatc aatgcaccgc ggccatccag gggttcggca acgtcggcat
     9961 gcacgccgcc acggcccttc agggctccgg tgtgcggatc gtcggcattg ccgacatcac
    10021 cggtggtatc aacgacccgg ctggattgga cgtacagcgc gtggccgtcc actacggccg
    10081 tcatggctcg ctcaccggct acaaggcccg ctccctgacc agccagcgct tgttccggct
    10141 tcccgtggac taccttatcc ccgctgcgac gggccacgtc atccacgcct ccaacgctgg
    10201 tcagatcaag gcccgcgtca tcgtcgaggg cgccaacatg cccgtcacgt acgatgcaat
    10261 gaaaaccctc gctgagaaag gcgtcgacct catccctgac atctacgcca acgcgggcgg
    10321 cgtcatcgca tctcatctcg aattccgcca ggccctgagc ggacaacgct ttacccgcgc
    10381 gatgacgctg gcccatatcc gtgaacgttt cgacgccacc tacgaggcca tgctgccgtt
    10441 catgaagaaa ggccgatcca tgaccgaagc gagcatcgac gttgcccttc aacgcgttca
    10501 ccaggccatg accgagcgcg aactcctggc aaggtagcgc gctccatgcg ggaaaaagcc
    10561 gcgggacatc ccagggagca ggctgcgaaa accatcatcg gatggtccga atacgttgcc
    10621 ctgccggaat ggggtatcag ccggctcccg gccaagatcg acaccggtgc gcgcacgagt
    10681 tcgctccatg tcgacaatct gcagatcgtt gatgcgcacc acgtctcgtt tgaagtggtc
    10741 ctgagccgaa agcacagcaa ccggcggatt cacgtcgtta ccagaatagc caagtggggg
    10801 cgggttcgct ccagcacggg ccattaccat acccgctgct ttgtgcacac ccgcgcccgt
    10861 atcggcgcca ttgagaaaaa catcgaactc tcactggcgt cgcgcgaaaa gatgctttat
    10921 cgtatgctac tgggccgaaa agccctcgaa aaggattttc tggtggatgt gtcccggcgc
    10981 cgtgtcctgg gcaaacccga aaaacccaga caaaaaaggt ctgtgccctc atgaagatcg
    11041 ggctcctcag ccgccagccg cgcagctaca gcacccggcg gctccgcgaa gccgccaagc
    11101 ggcgaggcca cagcgtccgg gtactcgata cgctccgctt cgcaatatcg ctcgaaaaac
    11161 aggaacccga cctatactac cggggccgga aactcccggc gttggacgcc gttatccccc
    11221 gtatcggcgc gtccattaca ttctatgggc tcgcggtgct ccgccagttc gaacaaatgg
    11281 gcatctacgt ccccaacgat tctgccggca tttcccggtc gcgcgacaag ttatgtgcca
    11341 cccagctgtt cagccgctac tccatcagca tccccgcaac ggaatttgtg cgcgacagca
    11401 gcgacatcct cccggcgatt cgccgcgtcg gtggcgtgcc ggtcattatt aaggtcatcg
    11461 aagggaccca gggcgttggc gtgatcctcg cggaaactga aaaagctgcc gaggccatta
    11521 ttcaaaccct gcatggcgca aaacaaaatg tcctcattca gaaattcgtc aaggagagcc
    11581 acggcagcga cctgcgcgcg tttgttgtcg gcggccgcgt tgtagccgcc atgcgccgcg
    11641 ttgccaagcc gggcgaatac cgcagcaatg tgcaccgcgg cgcctccgtc gaaccggtca
    11701 cgctcacgcc tgaattcacg cacaccgcgc tgcgtgcagc tcagatcgtg ggactgggca
    11761 tcgcgggcgt cgatatgctc gagtccgccg aaggccctca ggtcatggag gtcaactcat
    11821 cccctggtct tgaaggcatt gaaaaggcca cgggcatcga tgtggccagt gccgtaattg
    11881 agtttgttga agagcaggct ctgttccccg acgccgatct gcgtcagcgg ctgcgcctca
    11941 ccggaggggt cggaattgcc gaggtttccg tccatggcat gcccgaactg gagggcaaac
    12001 cgctgcgcga aacgccactg gccagccgta acatctacgt tctcaacatc gcccaccacg
    12061 acacggttgt cccgcacccc aaggcggatg atgtcatcca gactggcgac gtattgctct
    12121 gctatggcga tctgcgtgag ttacgtagta tgatgccgcc gcgcaggcgg cgaaaacgtc
    12181 cgcggaagcg cagcagttgg gagaagagca aagggtgagt gcccgaaatg gcctgaacgg
    12241 ccggacggcc cgggcaaccc ggccgttcga ggaacgcgag accctcgtga tcgccggtca
    12301 gagcattccg ctcggcgaaa cacgcgagct gcaattgcct tttgacgaaa cctacctggg
    12361 tgagcccgtc tccgtgccca tctatgtcat gcgcgctcca caacccggcc cgcgcgtctt
    12421 tctcacgggc acgctccacg gcgacgaact caatggcatg ggcattatcc gcgagctcct
    12481 ctacggcaac ccgctgcagc tcgttcgggg ctcgctcata ctcatccccg tcatcaacat
    12541 ccacggtctc gaacgccacg cgcgcaacat gcccgaccgc cgcgacccca accgctgttt
    12601 tcccggctcg gcgcgcggca gccagaccag ccgcatggcc cacgcgatct tcactgaaat
    12661 cgtcaagcaa tgcgattacg gcatcgactt ccacagcgcc gccatacggc gcgtcaacta
    12721 tcccaatgtc cgcgcctaca cccgcaatct caagacgcgc atgcttgccg aagcctttgg
    12781 ttgcgagttg atcatcaaca accaggggcc gcagggctcg ctgcggcggt ccgcggtcca
    12841 agccggtgta ccgtgcatca tcctcgaagc cggcgaagtc tggaagatcg aacccacggt
    12901 ggtcgaaatc ggcgtccggg gcgtcctcaa tgtcctgcgc actgtcggga tgctccaggg
    12961 cgaaccgcga aaaccgccat ttcagttgat cgtcaccaag acctcctggg tccgcgcccc
    13021 caccgggggc atcctcgatt ttcacgcccg gcctggcgat ctcgtccgtc gcaacgaatg
    13081 cctggccacc atctccagcg tcttcggccg ggagcactca gcgcttctcg ctccggtaga
    13141 cgggatcgtg ctcggcatga ccacaatgcc cgcggtcaag cccggcggcc cggtctatca
    13201 tctggccgtc ctgtccaaac gcaatgtccg ggtggccctg caccatctca tgaatggcga
    13261 gcgccggggc atgtttgggc gtgtccgccg cgagctgagt tcggccatgg ctttccgtga
    13321 gcgcgaatcc cggtacggcg acgcgaaatc tcagccgtga acccttttcc cgtacgccag
    13381 ccctgcattg cggcaggctc tctttcaggg cagctgcccg gttctctgta gtgctttctt
    13441 gacatccctg catcggtgga ttatcactaa gtcacatctg gcaggcgcct ggcgatgaga
    13501 aggggggaag gatggacaca ctcggcatgg ggatcgtcgg atgcggatgg gtcgcggaag
    13561 aatacattaa agccgcccag tgcgacgggc gtgttgaagt acgtgccctc gtcagccgga
    13621 cgcccggcaa accggaaacc taccgtcagc gctacaatct cgatgccact atcggcaacg
    13681 actacgaaga tctgatgcag cgtgacgaca tcgatatcgt tgtcgtttcc acgccccacg
    13741 acctgcatac cggccccgta gtcgcggccg ccgaagccgg taaacacgtc atcatcgaaa
    13801 aaccggttgc cctcaccatg gatgatgtcc ggaaacagca ggccgccgtc cagaaaaccg
    13861 gcgttaagac gatcgtcagc tttgtgctca ccaggaaccc cctgctcatc agcatcgacg
    13921 ccctgatccg cgacggcgcc ctcggcaacg tgtttctggt cgaagtagac tatctgcacc
    13981 gcatctggtg cggttccgag aaatggctcg gcagaaaaga caaggccgga agctcgtttc
    14041 tcgcgggtgg gtgccacgcc gtaagcgcca tgcagtggtt cgcacggagc gaagccgtcg
    14101 aagtctgcgc gtattccgtc cagaccgaaa accccaacga ctatcccggc accaccaccg
    14161 cggtcgtcaa atttgccgac ggcacgctgg gccgcacgac aagctgcttc gacgcccaaa
    14221 tgccttacgt cttccacatc ggcgtctatg gcaccgaagg ttccgtgcgc aacgacaagg
    14281 tctttgctcc caagattttt cccggccaga acgcgttcat gaccgtgccc tgcgttctgc
    14341 ccgacagcgg cgacgtcgcg caccatccct tccagggcga ggtctctcat ttcatcgatt
    14401 gcatcctcga ggacaagaaa ccgttcccga acctcgacga cgccgcgaaa accatggcca
    14461 tctgcatcgc tgccgacatt tccagcgttg aaggccgccc agtgaagacg accgaggtct
    14521 gacgccggga cccgtccatg cgggccgcgt gtgtcgacgc ctcatgcgga tttgtggtac
    14581 cgtcatccgg gatgccgcca ggggcggcgt catcgtgcat aggagttgca ccatgaccga
    14641 acggactcag aacggactca accggcggga atttttcgtg catacaggcc ggggtgcgat
    14701 ggtggggggc attctcggca cgactggccc ggcgcgcacc gtgctcgctc aaacgcagga
    14761 gtcctctcaa ccctctgccg cggcgtctgc gggctcactg caggtgggct gggctgccgc
    14821 cagcatcaca cccgaccagc ccgtccagct ccatggccag tttcacgagc gcgtgtccaa
    14881 atatgtgctt gatccgtgca tggccacggc cctggctctc gagcgcgacg gcgaacaggc
    14941 ccttatggtc agctgcgata tcgtgaacgt gcagcgtgct gtcacggaag aaatccgcag
    15001 ccgcgtagcc cagcagctgc ccggatttga tcccagcaag ttttttatca atgccaccca
    15061 tacccatacg ggtccgacga tgtccgtcgg cctgttcaag gaccccgatc cgggcgtgat
    15121 caccccccag gaatacgtcg aattttttac acaacacgtc acggaggcgg cggtcgccgc
    15181 ctggaacgca cgccgcccgg gcgccgtcag ccgtgccagc ggccaggccg ccatcggatt
    15241 caatcgcgtt gccaaataca aagacggcag ttcccagatg tacggcaaat cggaccgccc
    15301 cgattttcag ggactggagg gcggcgacga ccacgggctc gaaattctct tcttctacga
    15361 cgacaaacaa gtcatgaccg gcacactcat caacgtcgca tgcccatctc aagtcgtcga
    15421 ggggcaatat ttcgtctcgg ccgacttctg ggggcctgcc cgcgaacagc tgagagcgct
    15481 ctatggcgag cagttgtacg tatacgccat ggtcagtgcc gccggcgacc agtcgccccg
    15541 cgacctggtc cgccgcggcc gcaacgagcc cgatatgcgg gacattgccg gccagcagga
    15601 aatggcgcgg agactggtca acgccgtgca gatcgcgcac ggtaccacga agacgccgcc
    15661 cgtaaacgac ctcgaattcc gccatcacgt cgaggaactc gagctcccgg cgcgaaaagt
    15721 cactgatgac gaagtcaccg cggcccgcga ggagattgcc cggctgacga aggacggccc
    15781 gcctcaaccc acgacgagcc aggcgggaac agtccggcgc atgaacaggc tgatcgaacg
    15841 gcacagcgcc cagggcgatg ctcccgctta ttcgatggac ctccacgtca tccgcctggg
    15901 cgacatcgcg atcgccacca atcccttcga gttgtatctc gaatacggca gccagatgaa
    15961 ggcacggagc cgagccgagc agaccttcat cgcccaactc accagcgatc gcggcggcta
    16021 tctgcctacc cgggcggcgc tgcccggaaa agcctatggc tcacgtgtca ccgaaaatac
    16081 ggtcggcccc gaaggaggcg aactgctcgt cgagcggacc gtcgagatca tcaacagcat
    16141 gtgggaggcg tgagggaaaa ccgaagcacg gagcggtgct tctatcgcga gcgccacaac
    16201 gcaggaggga cgttccatgg ctgacattca ggttggattg aacaccgagg ccttccggca
    16261 cgcggacaaa cccctcgaat atgcactcga ggccatccgc aaacaaggct accgctactg
    16321 cgaactcaac atgctcaatg gccgcgacct gctctgtgaa gcgggatatt acgccgcggt
    16381 aagcatggaa cgcgacccgc tcgaagtgaa agccatcgtc gacagcttcg gcctcaaggt
    16441 cagcgcggtc agcgcccacg tgccgatggc gcagcccgag atcagcatcc cgttcctcac
    16501 ccgcgccatc caattcgccg ccggactcgg cgccacatgc gtctgcactg acgaaggcat
    16561 ggttcccggc tggatgaaca aaacccaggc attcaacctc atttactaca ccctgcgccg
    16621 ggtcttgccc gtcgccgaac gccacaaaat ccatatcgcg ctcgaacctc atcaggtcta
    16681 ttccgtcaaa ctcaaaacct acctcgagat cctcaatctc atcgattcgc cctacatgag
    16741 ctgcaatccc gacacgggca acatcttcat ggccggccag gatccctacg agttcctgga
    16801 ggcgattgcc aaacgcgtcg tccacgtcca cgccaaagac atcggcggtg tggtcatgag
    16861 cgaccgcggc aaagtcaccg gcgtgccctc cggctgcgcc tgcggcgaag gcgtcctcga
    16921 ctggcagcgg atcgtcagta tcctccgcaa agccaagttc aaaggcgtcc tctcggtcga
    16981 atgcagctcc gaagaacagg gccgggccag catcaaatac ctcaagaagg tcctggcaca
    17041 gaaatagccc gtttttcgca accggccacg gcgcggagac ccatgggggt ctccgcgatt
    17101 ccctcggttt gcgcccaaac gaaatgctgt gctaaactat gtccttactt tgctttggcc
    17161 agtgtgacgg ggcgtagaga gcgccatatc aggagaatgg aagtgaaaaa gggtatccat
    17221 cccgactaca aggcggcacg ggtcacgtgc gcttgcggca atacgttcga gaccaagtcc
    17281 accaaaggcg acatcaacgt ggaaatctgc tcggaatgcc atcccttcta tacggggaaa
    17341 cagaaattcg tggacagcga agggcgcgtc gatcgtttca accggaaata tgggcgcacg
    17401 agttaattcc cttttatgga aaaggtcatg cgtgagaagc ttcgcgacgt cgtcgcggag
    17461 tacgaccgcc tatcccgcga tatggcttcc caggaagtcg cctcggatcc cgaccgttat
    17521 atgaagtgtg ccaaggctca gaaacacatt gagccgctgg tcgactgctt caaaaaatac
    17581 gaaaacgaag agaaccgcct ggaacaggcc gaggccattc tctacgaaga gtctgacccc
    17641 gagcttcgtc agatggcgga ggacgagaag cgcctgctca cggaggcgct cgaggcgcgg
    17701 gaactcgaac tcaagctgct cctcgttcct ggcgacccca atgatgagaa aaacaccatc
    17761 atggaaatcc gcgcggggac cgggggagag gaggcggctc tctttgccgc ggatctcttc
    17821 cgcatgtact cgcgctatgc cgagctcaag gggtggaagg ttgaaatcct gaactctcat
    17881 acaaccggca tggggggatt caaagaaatt tcgttcaagg tcgcgggcga tcgcgtctac
    17941 agcctgctca aattcgaggg cggggtccat cgtgtccagc gcgtgcccga aacggaggcc
    18001 cagggccgcg ttcacacctc ggccgttacg gtggcggtcc tccccgaagc tgaagcagtc
    18061 gacatcgcca tcgacgaaaa cgacctgcag atcgatgtct accgctcatc cggcccgggc
    18121 gggcagtccg tcaacactac ggattccgcc gtccgcgtga cctataagcc cacggggctc
    18181 gtggtaacgt gccaggacga aaagtctcag cacaagaata aagcgaaggc cctgatggtg
    18241 ctccgggcac gcctcctggc gcagaaacaa gaggaagaag ccgctgaacg ttccgccaac
    18301 cgcaagagcc aggtgggttc gggcgaccgg agcgagcgca tccgcaccta caattttcct
    18361 cagaaccgcc tcactgatca ccgcatcaag cttacggtgc accgtcttga aacagtcatg
    18421 gaaggc
//
LOCUS       DAOPBQ010000009        17737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_3280_length_17737_cov_3.853127, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000009 DAOPBQ010000000
VERSION     DAOPBQ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 17737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17737
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3280_length_17737_cov_3.853127"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1200)
                     /locus_tag="PKW60_00720"
     CDS             complement(220..1200)
                     /locus_tag="PKW60_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPJ97981.1"
                     /translation="MNWRDRKVLITGAAGFIGSHLCERLLWAGADVRAMVHGNMRGSI
                     GHLAAIPEDLRKGLEIVGGNIRDGAFVRDATIGVDTVFHLAAITSVVYSYSNPDETVI
                     TNVSGTLNVCNAARHENVRRLVHTSSAGVYGTTAGDTPISETHPVRAYNPYTASKLAA
                     DNVVESFYLSYGLPVTIVRIFNVYGPRIGRFLIIPTIILQLLKGGDLKLGSLTPTRNF
                     TYVDDIVDAYLLMAEHESVVGEVVNFGSTEALTIAELAQLIARLMQREVTISQDDRAI
                     RPAKSEIERVLADVTKARQLLGWRPAVALEAGLKKTIDWIAAGGYDDLPA"
     gene            complement(1267..2367)
                     /locus_tag="PKW60_00725"
     CDS             complement(1267..2367)
                     /locus_tag="PKW60_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HPJ97982.1"
                     /translation="MRIAILGTRGIPANYGGFETFAQELSVRLVERGHEVCVYCRSSN
                     YPEPLEEYRGVRLITLPTIHTRAFDTLSHALLSTCHVLFQRADVVLYCNCATSCVAWM
                     PRLAGAKVVMNPDGLEWERAKWGWAGKAFYRFSEHLAARFPHRLVSDSQVIQAYYRDR
                     FGCESDFVAYGADLVERGTGKELLAEFGIEPERYFLYVSRLEPENNAHLVVEAFEQVE
                     TDMHLMVVGSAPFADDYIRRLTRTNDKRIIFPGALYGDIYRALQANAYAYVNAMEVGG
                     THPAILEAMGAGNCVLVSDIAYNVEAVAEAGVQFKSKDVGDCRAKMQWLADHPEEVSR
                     FRQHALDRARSQYQWDHVAAEYERLFEALLAG"
     gene            complement(2706..4442)
                     /locus_tag="PKW60_00730"
     CDS             complement(2706..4442)
                     /locus_tag="PKW60_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014230969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter"
                     /protein_id="HPJ97983.1"
                     /translation="MPSKEHAGVVSSLDKLYEFDREPVTEDKLQPGRYFAGLLAGEHV
                     AGTEFVIGASFVSWGASPMHVFLGLALGNLLAVLTWTFVCAPIATQTRLTLYWYLRKI
                     AGPAATAIYNVLNAIMFCILAGCMITVSASAVRIPFGIPPQVKWYPESAGFVVVVLIV
                     GALVTLLAILGFKRVAQFGAVCSPWMFLMFMGGAVATLPVLAMQAGFSNISTWAQFWE
                     AASKFIWTGSTPDGSPGMSFWQLTAFAWICNLAMHGGLSDMALFRYARHWSYGAYSAF
                     GMFFGHYLAWIFAGVMGAATARLMQVSMAQLDAGEVAWQALGVTGILTVIIAGWTTSN
                     PTIYRAGLAFQGVTPGWPRWLVTLLTGAATTIIACFPFVFTKLLNFVGLYGLLLVPVG
                     AIVFVEHWIFPRIGFTRYWVSRKRLFVSWPALISWGVAIAAALWLQWSGTLHLFSLFL
                     PIWFLTAILYTILAALCGAREALPEDDGIKTAEMLGASAKHGPQPTKTAQEPEQVDML
                     LWAARIVALLALAACLILPLWVYSKGVSGFEPSMALMKKILIWPTLIYFISGTVWAAR
                     RDRIKDPQGPET"
     gene            complement(4617..6167)
                     /locus_tag="PKW60_00735"
     CDS             complement(4617..6167)
                     /locus_tag="PKW60_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPJ97984.1"
                     /translation="MKEFVINVAPLETRIALLEDTRLAELTVERHENRSLVGNIYKGR
                     VDSIVPGIQAAFIDIGFEKNGFLYVSDIAGAEGTGDFEFDEGTAKPRKKSQRRKAPAI
                     ETIIKKNQYIMVQVSKDSVGSKGMRLTNYITLPGRYGVLMPTVNHLGVSRKIESERER
                     DRLKRILHQVRPQGLGLIMRTAAEGKSKADFQPDVQYLARLWTKICGKMESVKGSALL
                     HEDIGPILRIIRDAFTADVDRLTVDSEVEYERILNFLDSFAPSLQKRCKLYRGKMPIF
                     EKMGLESEVDKALNRKVYLKSGGHICIDQTEALIAIDVNTGRFTGSKNLEETVLLTNL
                     EAAEEIARQVRLRDLGGIIVIDFIDMQYERNRRELLKRLQECLKRDRAKTTMSEISEL
                     GMIEMTRKRVKHNLVKALSQPCPYCEGSGLVRSVTTMTFTALRRLESLFCRSKEKSVL
                     VQVHPDVARRLRHENKDLLDAIAGEFEREVSVESVSDFHIEDVAFLSARTRKEIVGGQ
                     GAIRGRAG"
     gene            complement(6203..6838)
                     /locus_tag="PKW60_00740"
     CDS             complement(6203..6838)
                     /locus_tag="PKW60_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HPJ97985.1"
                     /translation="MSVLRVRLTRGQALRGLIFRKFVDSVRGAVERAGLPVARNRRGA
                     PRLEAGPHLAPAHTSRCEYIDFELTDPITGREFLRRLSMELPAGVDAVSARRLPPGSK
                     NLKAAVRVLRYRVAGDFRPEQAERFRRASSWPVVRIRKGQERCLDLRRSVARLDVGPG
                     EVTMEIEVRVDGTPRPEEVIASVFGIPLEAALLLETERSGMLLSGTSPARP"
     gene            complement(6867..8021)
                     /gene="rodA"
                     /locus_tag="PKW60_00745"
     CDS             complement(6867..8021)
                     /gene="rodA"
                     /locus_tag="PKW60_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPJ97986.1"
                     /translation="MSQIARDLNLMDSHIRIVNWRNLRHLDWVLVVLVGALAYMGLSN
                     MYSASYGYEGAESTSHFARQALFLGVGSLLALALVCVDYRFLVALAPVWYGLALLALL
                     AVLTVGLTVKGGRSWFRLGAFSLQPSEYAKLATIFMLAWYLSRTGPRIQKFQYLILAF
                     IVAAVPSALIAAQSDAGTALTYVPVVFAMVFVAGCRKRHLAAIILVGAALAPVAWMHM
                     QPYQRDRVKTFMDPTTAPDSEGYQIIQTKIAVGSGQMWGKGVGQGTQTHLRFLPEYRT
                     DFIFSLLAEERGFVGGTILIALFAGFLIRGLMLSEECREPAGSLLTAGCVAILAFHVF
                     VNVAITIHLMPITGLPLPFLSYGGSFYLTTMMCVGTMLSVRVRKGMFTEK"
     gene            complement(8018..9961)
                     /gene="mrdA"
                     /locus_tag="PKW60_00750"
     CDS             complement(8018..9961)
                     /gene="mrdA"
                     /locus_tag="PKW60_00750"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPJ97987.1"
                     /translation="MLVERDKPKRFEGAPGRLRLLMLGLLGAYSLLALRLWDLQIIHG
                     DEYAEASNENRLHFERLEAPRGIIYGRTEDVVLADTQPACDIMFVPADCREADRERVA
                     RRLAHLLAINPDNLLARIDQYRKKPFTQLPVKQNVSKTELARVEEYSFELPGVYTTVR
                     PLRRYHYGEVAGQVLGYIGEISREEIKARGQAFKMGDLIGRMGVEQMYDPILRGRDGQ
                     LCVVIYNQSEPQLRTDELGRPVVQTDSLGRRLHQELEFSRDPVPGKALSLTLDIGLQA
                     KCEELLGTERGAIVVLAADTGEVLAIASTPRYDPGVFVRTGYGHERKALLDARPSPMR
                     NRAFQEHFAPGSIFKVAMAIAGLEEGVISEHTTIGCDGYFYLPGVSRPWKCWRHKYGG
                     HGRPAVVDALAYSCDEYFYNVGIKLGIDNINKWSEMLGLGVVTGLDVPPGSEEKGLVP
                     SMEWKKAAFASAADPSERKWYPGETVNVAIGQGQVTVTPLQAAVMMAAVVNGGHRVRP
                     YLNKSLGPSLTPLPISDKTLEVVTRGLRKCVEKTERPSAGTGIEARVPGMIVLGKTGT
                     AQVVRFKHYDNELDIPYNERDHAWFVAGVPEEEPPIAISVLIEHGLHGSSAAAPIAKQ
                     IIEYFYSHLPADLRLAKAAEDGP"
     gene            complement(9972..10478)
                     /gene="mreD"
                     /locus_tag="PKW60_00755"
     CDS             complement(9972..10478)
                     /gene="mreD"
                     /locus_tag="PKW60_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPJ97988.1"
                     /translation="MIRKNIIWVVLVIATALIQTTWLAEENFRVMGVSPNLVLLVVIY
                     FAITEGEERAMFTGVLGGLFQDVGSDSVLGHHILCYVVVGFLTGRIAVRLITQHPAVK
                     VGLVCIAGTVDGILYSGIASVQNAQWMVFSDLKRIVVPATLYTALLTPVVFFLMDRLF
                     HRFFAAQT"
     gene            complement(10480..11403)
                     /gene="mreC"
                     /locus_tag="PKW60_00760"
     CDS             complement(10480..11403)
                     /gene="mreC"
                     /locus_tag="PKW60_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPJ97989.1"
                     /translation="MSLKRRIREHRPIIILAVLVVLCLSSLAVGKRGSLVTRVVRNAI
                     SVASHPFWLALDFVEESYDYTAAFVLDYHRHYREAQRLQAEFAEATQRLSEFKELKAE
                     RERLRTMLDFERSETVATLLPAKIISRTLGAFTIDRGRMHDVAPAMCVLAPDGAVVGV
                     VSEVSPFNATVLTLHNPDCKIGALIERNRVRGIVRGTGQVAEALCEMEYIDMKDQIGN
                     GDRVLTMGSGMYPAGRPIGTVIGVEAGKTLQKKARVLPDANLQSLDEVFVITQALIPA
                     DEMRGQPRPQGAVSRAYPMPDERPPQERYAP"
     gene            complement(11472..12500)
                     /locus_tag="PKW60_00765"
     CDS             complement(11472..12500)
                     /locus_tag="PKW60_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPJ97990.1"
                     /translation="MFGFLNFLPGLFSHDMGIDLGTANTLVYVKGQGIVLSEPSVVAV
                     NKDTGRVRAVGNEGKNMIGRTPGNIVAIRPMKDGVIADFEITEAMLRYFIQKVHNRRR
                     LAWPRVAVCVPSGITAVEKRAVTESAMQAGAKKVFTIEEPMAAAIGAGLPVQEPQGCM
                     IVDIGGGTTEVAVIALGGIVFCKSVRVAGDEMDQAIIQHLKRTYNMMVGERTSEEIKI
                     TIGSAFPLKNELEMQVKGRDQVMGLPKILTITSEEIRDALREPVGAIVDAVRVTLERT
                     PPELSADIVDRGVVLAGGGALLRGLDQLLSKETGLHVSVAEDPLTAVVLGTGKYLEGL
                     KSFQDEEL"
     gene            12858..13931
                     /locus_tag="PKW60_00770"
     CDS             12858..13931
                     /locus_tag="PKW60_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HPJ97991.1"
                     /translation="MYFVAIALLVLAGAENTQETPGERNSLIYDYFQQAADATTPALP
                     WNASSSGEHDAWRRAFEPEFLRLLGRNPEPVPLNVEWTDEKMETALFTRQKIYVQSEA
                     DYWVPVYYFVPKNSAGKRPAMICLHGHSGILPYIDEGTDQERQKSREHELDYAVKFAE
                     AGYVSAAIVQRGWNETRHETPHSCHRVTMDAFLLGMTPVGLRTWDAMRVVDFLQTQDN
                     VDPDRIGAAGLSGGGTTTLFLAARDERIRLAMIAGYYCTFRDSIFTIHHCICNCVPGI
                     MQVGEMSDVAALIAPRPVLIISGDEDTIFPIDATRRAYAALEKTYAVLGASGNLESDF
                     FEGGHQWSNRKSLDFLKTHFGTP"
     gene            complement(13935..14798)
                     /locus_tag="PKW60_00775"
     CDS             complement(13935..14798)
                     /locus_tag="PKW60_00775"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97992.1"
                     /translation="MEEEQDDILQRLESVIDSWTTEVDEAQRRLAEQLAETRGRLQNH
                     AETATESLQFDIDSNFAILQRLLEDCREAVRDAFSRLDVLEAEVQQLKDEVAELRESK
                     EAAPAPELKSEPESPKPMAWRRTRKVTIQPFDETGAPRRMGQILVDAGLITAEQLETA
                     VALQQESPGRKLGSILVEQGYTGEDVIPQVLARQLRLRYIRLREGAFDPRAVSLIPGD
                     LAEKHACIPVRATKDTLALAMANPLDIIAIEDVEKASGRRVQPIIARESEVLAAIAQW
                     YEAEEGSGADS"
     gene            15100..15606
                     /locus_tag="PKW60_00780"
     CDS             15100..15606
                     /locus_tag="PKW60_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HPJ97993.1"
                     /translation="MSIGLFYGSTSGNTQRIAEMIQAQYPGSVDLHKIHEDFDPEIFD
                     RYDAIIFGVPTYGQGSLQEDWADFIWEMDDIDLSGKHVALFGLGDQGGYPNSFADAML
                     ELYNKVQELGGAVVGTWPASGYAFRRSRAVVDGEFVGLVLDVDCQAEQTQERLTQWLG
                     QIAREFAN"
     gene            15650..16507
                     /locus_tag="PKW60_00785"
     CDS             15650..16507
                     /locus_tag="PKW60_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPJ97994.1"
                     /translation="MITCLVFLTQLAAPAESAGPPFVHTQNVVYGEAHGIGLIMDVFV
                     PRGEKNGAAIIDVVSGAWYSDRNKLAEHWGVGMYDFHCARGFTVFAVRPGSVSHFTGE
                     EMLAHIRMALKFVRDQAAAYGIDPQRIGMVGASAGGHLAALAAVTAAPEDRIQALAVF
                     FPATDFLNWDGDGQMPDFKEIGPLLFRCGLEGHSEEEIHAKAAELSPVRQIREVPPPC
                     LLIHGDADPLVPLSQSQALLDALKRAGGQAELIIKPGGGHPWPTIRDEVAVMADWMER
                     QLGAPSSAE"
     gene            complement(16526..>17737)
                     /locus_tag="PKW60_00790"
     CDS             complement(16526..>17737)
                     /locus_tag="PKW60_00790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 domain-containing metallophosphoesterase
                     family protein"
                     /protein_id="HPJ97995.1"
                     /translation="WNRKRSKLMNQSASRRTFLKTAGAVAAAMALPARGQETSAAPAP
                     KIVAGPYLQNVSPEAATVMWITDKPCVSRVDYGPTRELGNRVQNSHHGLVDAHDLIHR
                     MHLTGLTPGHPCYYRVSSREITLFEPYKVEFGDQVEDAVRQFTPPLPGAGRVDFAVFN
                     DIHETAETWARVHELAGQAPCDFLVFNGDLLNHAQDHGQVTRRFLAPATGPLEGARPF
                     VFVRGNHEARGVYARHLLDYFDSPDGGYYHAFTWGPVHFLVVDTGEDKLDESNEYFGL
                     ANFKPYLEKEAAWVAEQVKSDAFRQAAFRVVLCHIPLRPSRPNDYRLPIIEALNGTGV
                     DLSISAHMHKPAYHEPEPGRDFPIVVGGAPKPAEATVIRVEAEPTEMRLQMTLADGTL
                     LESRHYSARRA"
BASE COUNT         3386 a         6009 c         5072 g         3270 t
ORIGIN      
        1 ttcatccagg cggaatccat ggtgacggag cgtttcctgc ccgcccgccg aaccggccgc
       61 cagcgcgcag agcacaccga atgccgcagc cacccaccgc ccccgcgggg tgcgcggtgg
      121 cgcgctggtg cgggcgcctg ctcttcgttc actaccagcc atggctgcca tccaaagtcc
      181 tgctcccgaa accgtcaaac ctctccaaca tcggccgcat caggccggca gatcgtcata
      241 gccccctgcc gcaatccagt ctatcgtttt cttgaggccg gcctccagcg ccaccgccgg
      301 gcgccagccc aggagctgcc tggccttcgt gacatcagcc aacacgcgct cgatctcgct
      361 cttcgccggc cgaatcgccc ggtcgtcttg cgaaatggtc acctcgcgct gcatcagtct
      421 cgcaatgagc tgcgcgagct cggcgatcgt cagggcctcc gtcgacccga agtttaccac
      481 ttcgcccacc acactctcgt gttccgccat cagcaggtag gcatccacga tgtcgtccac
      541 atacgtgaaa ttgcgcgtgg gcgtcaggct gccgagcttg aggtcgcccc ccttcagaag
      601 ctgcaggatg atggtcggaa taataagaaa acggccgatc cgggggccat atacgttgaa
      661 aatacgcacg atcgtcacgg gcagaccata cgaaaggtag aaactctcca ccacgttgtc
      721 cgccgccagc ttcgacgccg tataggggtt ataggcccgc accgggtgtg tttcgctgat
      781 cggcgtatcg cccgccgttg tcccatacac acctgccgac gaggtatgca caagtcgccg
      841 cacattttcg tggcgggccg cgttgcacac gttcagcgtt ccgctcacgt tggtaatcac
      901 ggtctcgtcc gggttcgaat acgaatagac cacgctcgtg atcgcggcca gatggaacac
      961 cgtatccacc ccgatggtcg catcgcgcac gaaggccccg tcacggatgt tgccgccgac
     1021 gatctccagg cctttacgca ggtcttccgg aatagccgcc agatgcccga tgctgccccg
     1081 catattgccg tggaccatcg cgcgcacatc ggcgcccgcc cagagcagcc gttcacagag
     1141 atggctgccg atgaatcccg cggctccggt gatcagcact ttcctgtcgc gccaattcat
     1201 acgcgctctc cagcctggcc ggcgccgctt acagcacgaa cggcggcccc ggcctgcact
     1261 acacgtttaa cccgcgagca acgcttcaaa aagccgctcg tattccgctg cgacatggtc
     1321 ccactgatat tgagaccgcg cccgatcgag ggcgtgctgc cgaaaccggc tcacttcctc
     1381 tggatggtct gccagccact gcatcttcgc acggcagtcg cccacatcct tacttttgaa
     1441 ctgcacgccc gcctcagcca cggcctcgac gttgtacgcg atatcgctca ccagaacaca
     1501 gttccccgcg cccatcgcct cgaggatcgc cgggtgcgtc ccgccgacct ccatggcgtt
     1561 cacgtaggcg tacgcattgg cctgcaacgc ccggtagatg tcgccgtaca gcgcacccgg
     1621 aaagatgatc cgcttgtcgt tcgttcgcgt caagcgccgg atatagtcgt cggcaaaggg
     1681 cgcgctgccc accaccatca gatgcatatc cgtctcgact tgctcgaagg cttcgaccac
     1741 caggtgtgca ttgttctcgg gttccagccg gctcacatac aaaaaatacc gctccggctc
     1801 aataccgaat tcagcaagca gctctttccc ggtgccgcgc tccaccaggt ccgcgccata
     1861 cgccacgaaa tcactctcgc agccgaatcg atcccggtaa taggcctgaa tcacctggga
     1921 atccgacacg agccgatgag gaaagcgtgc ggccaggtgc tccgaaaacc ggtaaaacgc
     1981 cttgccggcc cagccccatt tcgcccgctc ccactccagt ccatcgggat tcatgaccac
     2041 tttggcccca gccaggcgcg gcatccacgc aacacagctg gtggcgcagt tgcagtacaa
     2101 cacgacatcc gcacgctgaa ataacacgtg gcaggttgac agcagggcat gtgagagtgt
     2161 gtcaaaggcc cgggtatgga tcgtcggcag cgtgatcaga cgcacccccc ggtattcctc
     2221 aagcggctcc gggtaattgg aactgcggca atagacgcac acttcgtgcc ctcgctccac
     2281 gagccgaaca ctcagctctt gggcgaacgt ttcaaacccc ccgtaattcg ctggaatgcc
     2341 gcgggttccc aggatcgcga ttcgcatgcc ggtagcacgt gtcctcagta aatgcgcgcc
     2401 cctgctttgg gttcagggct ggcgtactgc caaggcgcta aagtaagaat accacaaatg
     2461 aggctttatt tgtcaactaa atccctgtca gcgtgccgtt ccggccgcac ctggggatca
     2521 ggagctgcgc tgcggcggca gggcaggggg ccggcggcag cgtgttcatg cggtctagtt
     2581 ttgccactca accgggaacg gcggcgaaac cccgtggctc cggcgcggcg cccagggagg
     2641 gtttcgttcc agatacgcgg tcttgcccca gcatctcagc gtggctcgtt cagctccccc
     2701 cgggctcacg tctccggacc ctgcgggtct tttatccggt cgcgccgggc ggcccatacc
     2761 gtcccgctga tgaaatagat cagggtgggc cagatgagga tcttcttcat tagcgccatg
     2821 ctcggttcga aaccgctcac gccctttgaa tacacccaga gcggcaggat gaggcaggcc
     2881 gcgagggcca gcagcgccac aatgcgggcg gcccatagaa gcatatccac ctgctccggt
     2941 tcttgcgcgg ttttcgtggg ttgggggccg tgtttcgcgc tcgcaccgag catctccgcc
     3001 gtcttgatcc cgtcgtcttc gggcagggct tcgcgcgcac cgcacagggc ggcgaggatc
     3061 gtgtacagaa tcgccgtcag gaaccagatg gggaggaaca aagagaacaa atgcagggta
     3121 ccggaccatt ggagccacaa agctgccgcg atcgccacgc cccagctgat gagcgccggc
     3181 cagctcacga agagccgttt gcgcgagacc cagtaccgcg tgaagccgat ccgcgggaaa
     3241 atccagtgtt ccacaaacac gatcgccccg accggcacca gcagcaggcc gtacaggccg
     3301 acgaaattca gcagcttcgt gaagacaaac gggaagcagg cgatgatggt ggtcgcggcg
     3361 cctgtcagca gcgtgaccag ccagcggggc caccccggcg tcactccctg aaacgccagg
     3421 cccgcccggt agatcgtcgg attcgacgtc gtccagcccg caatgatgac cgtgaggatg
     3481 ccggtgacgc ccagggcctg ccaggccacc tcgcccgcat cgagctgggc catgctgacc
     3541 tgcatcaggc gcgccgtggc cgcgcccatc acgccggcga aaatccacgc cagatagtgc
     3601 ccgaaaaaca tgccgaaagc cgaataggcc ccgtacgacc agtgccgcgc ataacggaac
     3661 aaggccatat ccgacaggcc gccgtgcatc gcgaggttac agatccacgc aaacgccgtg
     3721 agttgccaga acgacatccc gggtgagccg tcgggcgtcg agcccgtcca gatgaacttg
     3781 cttgcggcct cccagaactg agcccaggtc gagatattcg agaaccccgc ctgcatcgcc
     3841 agcaccggca gcgtggctac tgcgccgccc ataaacatca ggaacatcca cggcgaacac
     3901 acggcgccaa actgggccac acgtttgaag ccgaggatcg ccagcagcgt caccagcgcg
     3961 ccaacgatca gcaccaccac aacaaacccc gcgctctcgg ggtaccactt gacttgtggc
     4021 ggaatcccga acggaatacg cacggccgac gccgacaccg taatcatgca tcccgccaga
     4081 atgcagaaca tgatcgcatt cagcacattg tagatggccg tcgcggccgg gcctgcgatc
     4141 ttgcggagat accaatagag ggtcagccgc gtctgcgtcg cgatcggcgc gcacacaaag
     4201 gtccacgtca gcaccgccag cagattgccg agcgcgagcc ccagaaacac gtgcatcggg
     4261 ctggcgcccc agctcacgaa cgatgccccg atgacaaact ccgtgcccgc aacgtgctcc
     4321 ccggccagca gacccgcaaa ataccggccc ggctgcagtt tgtcttccgt tacgggttcg
     4381 cggtcgaatt catacagctt gtcgagactc gacaccactc cagcgtgctc tttgctcggc
     4441 atggccaaac catctccttg ctcgtggctt ccccgaaaaa cggcagggtc tccggtgtgg
     4501 gagcatcggc gccccatcac gcgcgggctt ccccttcccc cacccggcac tcaaaacccg
     4561 ctgccagtgt accccgccaa gcccgccatt cacaaatcgg ctcggctcgg cgccgcctat
     4621 ccggctctgc cgcgaatcgc cccctgcccg cccacgatct ctttgcgggt gcgggcgctc
     4681 agaaacgcca catcctcgat gtgaaaatct gaaaccgatt caaccgatac ctcgcgctca
     4741 aattctccgg cgatggcgtc gagaagatcc ttgttttcat gccgcagccg gcgcgcaacg
     4801 tccgggtgca cctgcaccag caccgatttc tcctttgagc ggcagaacag gctttccagc
     4861 cgccggagtg cggtgaaggt catggtcgtg actgagcgca ccagtccgct cccttcgcag
     4921 tacgggcagg gctgcgacaa ggctttgacc aggttatgtt tgacgcgttt gcgggtcatt
     4981 tcgatcatcc cgagttcgga gatctcggac atcgtagtct tggcccggtc acgtttgagg
     5041 cattcctgaa gccgcttgag cagttcacgc cggttccgct catactgcat gtcgatgaag
     5101 tcgatcacga tgatgccgcc caggtcgcgc agcctgacct ggcgcgcaat ttcctcggcg
     5161 gcttccaggt tggtcagcag cacggtctct tcgaggtttt tgctgcccgt gaagcggccc
     5221 gtgttcacgt caatcgcgat aagcgcctcg gtctggtcga tgcagatgtg tccgccgctt
     5281 ttgagataga cttttcgatt cagggccttg tccacctcgg attccagccc catcttttca
     5341 aatatgggca ttttgccccg gtacaatttg cagcgcttct ggaggctggg cgcgaagctg
     5401 tcgaggaaat tcaaaatccg ctcgtactca acctcgctgt cgacggtgag gcggtccaca
     5461 tccgcggtga acgcatcgcg gatgatccgc agtatcggcc cgatgtcttc atgcaggagg
     5521 gcggaaccct tcacggactc catttttccg cagatcttgg tccagagccg cgccagatac
     5581 tgcacgtccg gctggaaatc ggccttgctt ttgccctcgg cggcggtccg catgatcaat
     5641 ccgagcccct gggggcggac ctggtgcaga atgcgtttga gccggtcgcg ttcgcgttcg
     5701 ctttcaatct tgcgggagac gcccaggtgg ttcacggtgg gcatcagcac gccgtagcgg
     5761 ccggggagcg tgatgtagtt cgtgagccgc atccccttcg agcccacgct gtccttgctc
     5821 acctgcacca tgatgtactg gttctttttg ataatggtct cgatcgccgg ggccttgcgc
     5881 cgctgcgact tcttgcgggg tttcgcggtg ccctcatcga actcaaaatc gcccgtccct
     5941 tccgcgccgg cgatgtcgga cacgtagaga aaaccgttct tctcaaaccc gatatcgata
     6001 aaggccgcct ggatgccggg aacgatggaa tccacccggc ccttgtagat attgccgacc
     6061 aggctgcggt tttcgtggcg ctccaccgtg agttcggcca gccgcgtatc ctccaggaga
     6121 gcgatccggg tctccagcgg ggctacattg attacaaact ccttcattct gtctccgcat
     6181 tgccggcggc ggacgccgcc tgtcagggac gcgcggggga tgtgccggat aacagcatac
     6241 cgcttcgctc cgtctccagg agcagtgccg cttccagcgg gatgccaaac accgacgcga
     6301 tcacctcctc gggccggggg gtgccgtcca cgcgtacctc aatctccata gttacttctc
     6361 cggggccgac gtcgagcctc gcgacgctgc gccgcaaatc aaggcaccgt tcctgcccct
     6421 tccgaatccg gaccaccggc cacgagctgg cccggcgaaa gcgctccgcc tgctccggcc
     6481 tgaaatcgcc ggctacccgg tagcggagaa ccctcaccgc cgccttcaga ttcttgctgc
     6541 cgggcggcaa ccgccgtgcc gacactgcgt cgacccctgc gggcagctcc atcgacaggc
     6601 ggcgcaggaa ttcccggccg gtgatcggat ccgtcaattc aaaatcgata tattcgcatc
     6661 ggctcgtatg cgcgggcgcc agatgcggcc cggcctcgag ccgcggcgcg ccccgcctgt
     6721 tccgcgccac cgggaggccg gcccgttcga ccgccccgcg tactgaatcg acgaacttcc
     6781 tgaaaatcaa cccgcggagg gcctgccctc gcgtcaagcg gacacgcaat acgctcatcc
     6841 aggccttctc cgcaatccgt tcaacactat ttttccgtaa acatcccttt cctgacgcgt
     6901 acactgagca tagtgccgac acacatcatc gtggtcaagt aaaatgagcc gccatagctc
     6961 aaaaacggca aaggcagccc ggttatcggc atcaggtgaa tcgtgatcgc cacgttcaca
     7021 aacacgtgaa acgccaggat tgccacgcag cctgccgtga gcaggctgcc ggcgggctcc
     7081 cggcattcct ccgacaacat aagccctcga ataaggaatc cggcgaacag cgctatgagg
     7141 atggttccgc ccacaaaacc gcgttcctcc gccagaagcg aaaagatgaa gtcggtgcgg
     7201 tactcgggca ggaaacgcag atgggtctgc gtaccctggc ccacgccttt gccccacatt
     7261 tgtccgctgc ccacggcgat cttggtctga atgatctggt acccttcgct gtccggggcc
     7321 gtggttgggt ccatgaaggt cttcacccgg tcgcgctgat agggctgcat atgcatccac
     7381 gccaccggcg ccagcgccgc ccccacaagg ataatcgccg caagatgccg cttccggcag
     7441 cccgccacga agaccatggc gaaaaccacg ggcacgtagg tgagtgccgt gccggcatcc
     7501 gattgcgccg cgatgagcgc gctcgggact gccgcaacta taaacgccag gatgagatat
     7561 tgaaatttct ggatccgcgg gcctgtccgc gacagatacc aggccagcat gaaaatagtg
     7621 gccagtttcg catattccga cggctggagg ctgaaggccc cgagccggaa ccagctccgg
     7681 ccgcctttca ccgtgagccc cactgtcagt accgccagaa gcgccagaag cgccaatcca
     7741 taccacaccg gggccagcgc caccaaaaac cggtagtcaa cgcaaacgag ggccaatgcc
     7801 aacagactcc ccacccccag aaacagggcc tggcgggcga aatgcgaggt cgattccgcg
     7861 ccttcatatc cataactggc gctgtacatg ttcgacaggc ccatatacgc caacgccccc
     7921 accagaacca ccagaaccca gtccagatgc cgcagattgc gccaattgac aatgcggatg
     7981 tgtgaatcca tcaggttcaa atcgcgcgct atctggctca tggcccgtcc tccgcggctt
     8041 tggccagacg cagatccgcc ggcaggtgcg agtaaaaata ttcgatgatc tgcttggcga
     8101 tcggcgccgc ggctgaactc ccatgaagac catgctcgat cagcacgctg atggcgatgg
     8161 gcggctcttc ttccggcacg cctgccacga accaggcatg atcgcgttcg ttgtacggga
     8221 tgtccaactc attatcgtaa tgcttgaagc gcaccacctg ggccgtgccg gtcttgccca
     8281 gcacgatcat gcccggcacc cgcgcctcga tccccgtacc cgcgctcggc cgttcggtct
     8341 tttcgacgca tttgcgcaag ccccgcgtca ccacctcgag cgtcttgtcg ctgatgggca
     8401 gcggagtcag gctcgggccg agcgatttgt tcaggtaggg ccggacccgg tggccgccat
     8461 tcacgaccgc cgccatcatc acggccgcct gcagcggcgt caccgtcacc tggccctggc
     8521 caatggccac gttaaccgtc tcgccgggat accatttgcg ttccgacggg tcggctgcac
     8581 tcgcaaaggc cgccttcttc cactccatgg acggcaccag ccccttctct tccgaaccgg
     8641 gaggcacgtc cagccccgtc acaacgccca gaccgagcat ctccgaccat ttgttgatgt
     8701 tgtcgatccc cagcttgatc ccgacattat agaaatattc gtcacacgag tacgccagcg
     8761 cgtccacaac agccgggcgc ccatgcccgc cgtacttgtg ccgccagcat ttccacgggc
     8821 ggctgacgcc gggcagatag aaatagccat cgcagccaat cgtggtgtgc tcgctgatca
     8881 ctccttcctc aagcccggcg atggccatcg ccaccttaaa aatcgagccg ggggcaaaat
     8941 gttcctggaa cgcccggttt cgcatcgggc tgggccgcgc atcgagcagc gctttccgtt
     9001 catgcccgta gccggtacgc acgaacactc ccgggtcata gcgcggtgtg ctcgcgatgg
     9061 cgagcacctc cccggtgtcg gccgcgagca caacgatagc cccgcgctcc gtacccagca
     9121 gttcttcgca tttcgcctgg agcccgatat cgagggtgag ggacagcgcc ttacccggaa
     9181 cgggatcccg gctgaactcc agctcctggt gcagacgccg gcccaggctg tctgtctgca
     9241 cgacgggccg gcccagttcg tccgtgcgca gctggggctc gctctggtta taaatgacaa
     9301 cgcaaagctg gccgtcgcgc ccccggagga tgggatcata catctgttcc acgcccatcc
     9361 ggccaatcag gtcgcccatt ttgaacgcct gaccccgtgc tttgatctcc tcgcggctga
     9421 tctcaccgat gtagcccagg acctgccccg ccacctctcc gtagtgatac cgccgcaagg
     9481 ggcgcaccgt ggtgtagacc cccggcagct cgaacgagta ttcctcaacc cgcgccagct
     9541 ccgtcttcga gacattctgc ttgaccggca attgcgtaaa cggcttttta cgatactggt
     9601 cgatccgtgc cagcaggttg tcggggttga tcgccagcag gtgcgccagc cggcgcgcca
     9661 cccgttcccg atcggcctcc cggcaatcag cgggcacgaa catgatgtcg caggcgggct
     9721 gggtgtcggc cagcaccaca tcttccgtgc ggccgtagat tatgccgcgc ggcgcttcca
     9781 ggcgttcgaa gtgcagccgg ttctcgttgg aggcctcggc atattcgtcg ccgtgaatga
     9841 tctgcaaatc ccacaggcgc agcgcgagca gggaatacgc ccccaggagg ccaagcatca
     9901 gcagccgcag gcgcccgggc gccccctcaa accgcttcgg cttgtctcgt tctaccagca
     9961 tcgtgcgtta cctacgtttg cgccgcgaaa aagcggtgga acaggcgatc catcagaaag
    10021 aataccacgg gggtcagcag ggcggtgtac agggttgccg gcacaacgat ccgcttcaga
    10081 tccgaaaaca ccatccactg ggcgttctgc accgatgcga tgccggaata caggatgccg
    10141 tccacggtcc cggcaatgca caccagcccc accttcacgg ccggatgctg cgtgatcagc
    10201 cgcacggcaa tccgccccgt cagaaacccc accaccacat agcacaggat gtgatggccc
    10261 agcacgctgt cgctgcccac atcctgaaac agcccgccca gcactcccgt aaacatcgcc
    10321 cgttcttcgc cctctgtgat tgcaaagtag atcaccacca ggagcaccag gttcggagag
    10381 acccccatca cccggaaatt ctcctccgcc agccacgtcg tctgaatcag cgccgtcgca
    10441 atgaccaaca cgacccatat gatatttttg cgtatcattt tacggtgcgt accgctcctg
    10501 cggaggccgc tcgtccggca tgggataggc ccgcgacacc gcgccctgcg gccgcggctg
    10561 gccgcgcatt tcatccgccg gaatcaacgc ctgggtgatc acaaacacct catcaaggct
    10621 ctgcaggttg gcatcgggaa gcacccgcgc ttttttctgc agcgtcttgc ctgcctccac
    10681 accgattacc gtgccaatcg ggcgtcctgc cgggtacatc cccgaaccca tcgtcaacac
    10741 ccggtcgccg ttgcctatct gatccttcat atcgatgtat tccatctcgc acagggcttc
    10801 ggccacctgc cccgtgccgc gcacgatccc ccgaacgcga ttccgttcga tcaacgcccc
    10861 tatcttgcaa tcgggattgt gaagggtcaa gacggtcgcg ttaaacgggc tgacttccga
    10921 gaccacgccc accaccgcgc catccggcgc aagcacgcac atggcgggcg ccacgtcgtg
    10981 catgcggccc cggtcgatcg tgaacgcccc cagggttcga gagatgatct tcgccggcag
    11041 cagcgtggcc acggtctcgg agcgctcaaa gtccagcatc gtgcgaagcc gttcccgctc
    11101 cgccttcagc tccttgaact ccgaaaggcg ctgtgtggcc tcggcaaact cagcctgaag
    11161 acgctgcgct tcccggtaat ggcggtggta gtccagcacg aaggcggcgg tataatcgta
    11221 ggattcttca acaaaatcga gggccagcca gaagggatgc gacgccaccg atatcgcgtt
    11281 gcgaacgacg cgggtcacca gtgagcctcg cttgcccacg gccagcgaag ataaacagag
    11341 caccaccagc acggcaagaa tgatgatggg acggtgctcg cgtatgcgtc gcttcaagct
    11401 caagcgtcgt catccaaacc ggtcattgcg gaagccgggc cagcagcgct gtgtgcggtc
    11461 aagccgatgg ctcagagctc ctcgtcctga aaactcttca acccttcgag gtatttgccc
    11521 gtccccagca ccacggccgt cagaggatct tccgccacgc tcacgtgcag gcccgtctcc
    11581 ttcgaaagca gctgatccag cccgcgcaac agcgcaccgc cgcccgccag gaccacgccg
    11641 cgatccacga tgtccgcgga cagctccggc ggcgtgcgct ccagcgtcac gcgcacggca
    11701 tcgacaatcg ccccgaccgg ctcgcgcaat gcatcccgga tctcttccga agtgatggtc
    11761 agtatcttag gcaggcccat cacctggtcg cggcccttga cctgcatctc cagctcgttt
    11821 ttcagcggaa atgccgaacc aatggtgatc ttgatctctt ccgacgtccg ctcgcccacc
    11881 atcatgttgt aggtgcgctt caaatgctgg atgatcgcct ggtccatttc gtcgcccgcc
    11941 acgcggaccg acttgcagaa cacgatgccg cccagcgcga tcacggccac ttcggtggtg
    12001 ccgccgccga tgtcgacgat catgcagccc tgaggctcct gcactggcag gccggcgccg
    12061 atcgccgccg ccatgggctc ctcgatcgta aacaccttct ttgcgcccgc ctgcatcgcg
    12121 ctttcggtga ctgcgcgctt ctcgaccgcc gtgatacccg acggcacgca gaccgccacc
    12181 cgcggccagg ccagacggcg ccggttgtgc accttctgaa tgaaatagcg gagcatcgcc
    12241 tccgtgatct caaagtccgc gatgacgccg tccttcatcg ggcgaatggc cacgatgttg
    12301 cccggggtgc ggccgatcat gtttttgccc tcgttgccga cggcccgcac gcgccccgtg
    12361 tccttgttga cggccaccac ggaaggctca ctcaggacga tcccctggcc cttcacatat
    12421 accagggtat ttgctgtgcc gaggtcgatg cccatgtcat gggagaacag ccccggaaga
    12481 aaattcaaga atccaaacac agttgcaccc ttcttttaca ctgggatcca tccccgagga
    12541 cagccataaa ccctcatatt ataaggcctt gtgtctgatt ccgtttcccc gggcaactca
    12601 tgatagcaga accgcccgtc aaatcaaaga gaattcagaa caaacccgcc agcctctgcc
    12661 acggtacggc cggcctttgt cttcccaggc cgccttcggt atagtgcctt gaaacacggg
    12721 cacaccgggc aaccaggact cgtcagtccc gtgtccatac ggttgcaggc gccgccgcag
    12781 acgtggcgct cacaccacat gaatgtgcgc gtgcgccgca cggttccgcg gccgcactcc
    12841 gcgcgggaga aaacgccatg tacttcgtcg ccattgcctt gctggtgctg gctggggccg
    12901 aaaacaccca ggaaacccca ggagagcgca acagcctcat ctacgactat ttccagcagg
    12961 ccgccgacgc caccaccccc gcgctgccat ggaacgcctc atccagcggg gaacacgacg
    13021 cctggcggcg cgcgttcgaa cctgaattcc tcagactgct cggaagaaac cccgaacccg
    13081 tgcccctgaa cgtcgaatgg acggatgaaa aaatggaaac cgccctcttc acccgccaga
    13141 aaatctatgt gcaatccgaa gcggactact gggtgcccgt gtattacttc gtccccaaaa
    13201 acagcgccgg caagcggccc gcgatgatct gcctccacgg ccattccggc attctgccgt
    13261 atattgacga aggcaccgat caggaacgtc agaaaagccg cgaacacgaa ctcgactacg
    13321 ccgtaaaatt cgccgaagcc ggctacgtct ccgccgcaat cgtgcagcgc ggatggaacg
    13381 agacgcgcca cgagacaccc cacagctgcc accgcgtcac catggacgcg tttctactgg
    13441 gcatgacgcc ggtcgggctt cgaacctggg acgccatgcg cgtcgtcgat tttctccaga
    13501 cacaggataa cgtcgacccc gaccgtatcg gcgcggccgg cctttccggc ggcggcacga
    13561 ccaccctctt tctcgccgcg cgcgatgagc gcatccggct ggcgatgata gcgggatatt
    13621 actgcacgtt ccgcgactcg attttcacca tccatcactg catctgcaac tgtgtccccg
    13681 gcatcatgca ggtgggtgaa atgagcgacg tggccgcact catcgccccc cggcccgtgc
    13741 tcatcatcag cggcgacgag gacaccattt tccccatcga cgcaacccgg cgcgcctacg
    13801 ccgcgctgga aaagacctac gccgtcctgg gggcatccgg caatctggag agcgatttct
    13861 tcgagggggg gcaccagtgg agcaatcgca aaagcctcga cttcctgaaa acgcacttcg
    13921 gcacgccgtg agcgtcagga gtccgcgccg ctcccttctt ccgcctcgta ccattgagca
    13981 atcgccgcaa gaacctccga ctcgcgggcg ataatcggct ggacccgcct tccggaggcc
    14041 ttttcaacgt cctcaatcgc gataatgtcc aggggattcg ccatggcgag cgcgagggtg
    14101 tctttcgtcg cccgcacggg aatgcatgca tgcttctccg cgaggtcccc ggggatcagc
    14161 gacaccgccc ggggatcaaa tgctccttcc cgaaggcgga tgtagcgcag ccgaagctgg
    14221 cgggccagca cctgcgggat aacgtcttcg cccgtgtagc cctgctccac caggattgag
    14281 cccagcttgc gcccgggcga ttcctgttgc agcgccaccg cggtctccag ctgttccgct
    14341 gtgatgagac ccgcatcaac caggatctgc cccatgcggc gcggcgcgcc ggtttcgtca
    14401 aagggctgaa tcgtcacctt gcgcgtacgc cgccatgcca tgggcttggg gctctcgggc
    14461 tcgcttttga gttcgggcgc aggcgccgcc tctttggatt cacggagttc ggctacctcg
    14521 tctttcagct gctggacttc ggcttcgagc acgtcgaggc ggctgaatgc gtcgcgcacg
    14581 gcttcccggc agtcttccag aagccgctgc aggatggcga aattggagtc aatgtcgaac
    14641 tgcagcgatt cggtcgcggt ctccgcgtga ttctggagac ggccgcgcgt ttccgcgagt
    14701 tgctcggcaa gacgccgctg agcctcgtcg acttccgtgg tccacgagtc aattacggac
    14761 tcaagccgct ggaggatatc gtcttgttct tcttccattg ttgctgttat ccgcggttga
    14821 cgctgccatc atgcatccgt atttgtctcc cggctgctcc ggcggcacgg gcgcacgcaa
    14881 gcctcccacg tctgaattgt agagtgcccc ggcgggcccg tcaaacaggc gatctccgac
    14941 cgtcaggtcg gcggcattca agcacgcgtc gccggaggcg tcaggaagag gccgtccctg
    15001 ctcccgatga cctcagacat gtttttcacg cggggattgt tcgtatacta aaaaaccctg
    15061 cggcttgtgc cgcgatcatg tcagaaggag cgatttttca tgagtatcgg cttgttttac
    15121 ggcagcacca gcggaaatac ccagcggatc gccgagatga tccaggcgca gtaccccggc
    15181 agcgttgatt tgcacaaaat ccacgaggat tttgacccgg aaatcttcga ccggtatgac
    15241 gccattatct tcggcgtgcc cacctacggt caggggtctc ttcaggaaga ctgggccgat
    15301 ttcatctggg agatggacga tatcgatctc tcaggcaaac acgtggccct tttcggtttg
    15361 ggcgaccagg gcggttaccc caacagcttt gcggacgcca tgctcgaact gtataacaag
    15421 gtgcaggaac tgggcggcgc cgtggtgggc acgtggccgg ccagcggcta cgcattccgg
    15481 cgctccaggg ccgtcgttga tggcgaattc gtcggcctcg tccttgatgt cgactgccag
    15541 gccgaacaaa cccaggaacg cctcacacaa tggctgggac agatcgcgcg ggagtttgcc
    15601 aactgaacgg gtagccgcgc gcgggcttca ccgccgggga gaagtagaaa tgatcacatg
    15661 cctggtattt ctgacccagc tcgcggcgcc tgccgagtcc gcgggtccgc cgtttgtgca
    15721 tacgcaaaac gtggtgtacg gcgaggccca cggcatcggg ctcatcatgg atgtctttgt
    15781 tccgcgcggt gaaaagaacg gcgccgcgat catcgatgtg gtcagcggcg cctggtactc
    15841 cgaccggaac aaactcgccg agcattgggg cgtaggcatg tacgatttcc attgcgcccg
    15901 cggtttcacc gtcttcgcgg tacgccccgg ctccgtctcc cattttaccg gtgaggaaat
    15961 gctcgcccac atccgcatgg cgctgaagtt cgtgcgcgac caggcggccg cctacggcat
    16021 cgacccccag cgcattggca tggtaggcgc ctctgccggt ggccatctcg ccgcactggc
    16081 tgcggtcacc gctgctccgg aggaccggat ccaggcactc gccgttttct ttccggccac
    16141 cgacttcctg aattgggacg gtgacgggca gatgccggac ttcaaggaga tcggcccgct
    16201 gcttttccgg tgcggccttg aggggcattc cgaagaggag atccacgcga aggccgcaga
    16261 actctccccg gtccggcaaa tacgcgaggt gccgccgccg tgcctgctca ttcacggcga
    16321 tgccgatccc ctggtgcctc tgtcgcagtc gcaggccctg ctcgacgccc tcaagcgcgc
    16381 gggcggccag gccgaactta tcatcaaacc cggcggaggg catccctggc ccaccatccg
    16441 cgacgaggtg gcggtcatgg cggactggat ggagcgccag ctcggcgcgc catccagcgc
    16501 cgaatagact cccgcggcgg ccggatcagg cgcggcgcgc ggaatagtgt cgcgattcca
    16561 gcagggtccc gtcggcgagg gtcatctgca ggcgcatctc cgtgggctcg gcttcgaccc
    16621 ggatgaccgt cgcctccgca ggtttggggg ccccgccaac gacgatggga aaatcgcggc
    16681 ccggctccgg ctcgtggtat gccggcttgt gcatgtgggc ggaaatcgac aggtcaactc
    16741 ccgtcccgtt aagcgcctcg atgatcggca ggcgataatc gttcgggcgc gagggcctca
    16801 acgggatgtg gcacaagacc acccggaaag cagcctgccg gaaggcgtcg ctcttgacct
    16861 gttccgccac ccacgcggct tccttttcga gatagggttt gaaatttgcc agcccgaaat
    16921 attcgttgct ctcgtccagc ttgtcttccc cggtgtccac cacgagaaaa tgtacgggcc
    16981 cccaggtgaa ggcgtggtag taacctccgt ccggcgagtc aaagtagtcg agcaaatgcc
    17041 gcgcatacac tccgcgcgct tcgtggttgc cgcgcacaaa cacgaacggg cgggcgcctt
    17101 ccagcggccc ggtggccggt gcgagaaagc gcctggtaac ctgcccgtgg tcctgggcat
    17161 gattcagcaa atccccgttg aacacgagga aatcacatgg ggcctgcccg gccagctcgt
    17221 gaacccgcgc ccacgtttcc gcggtctcgt gtatatcgtt gaacacggca aaatccacgc
    17281 ggcccgcccc cggaagcggc ggcgtgaatt ggcgcacggc gtcttccact tgatcgccaa
    17341 attccacctt gtacggctca aacaaggtga tctcccggga actcacgcgg taataacagg
    17401 gatggccggg agtcagcccg gtaaggtgca tgcggtgaat caggtcgtgc gcgtcaacga
    17461 gcccgtggtg gctgttctgg acgcggtttc ccagctcccg agtcggcccg tagtccaccc
    17521 ggctcacgca gggtttatcc gtgatccaca tcaccgtcgc cgcttcaggc gagacatttt
    17581 gcagataggg ccccgcaaca attttcggcg ccggcgcagc ggaagtctcc tgcccgcgcg
    17641 cgggcagggc catcgcggcc gcgactgcac ccgcagtttt caagaaggtt cggcggcttg
    17701 cagattgatt catcagcttg ctccttttcc tgttcca
//
LOCUS       DAOPBQ010000010        17718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_3287_length_17718_cov_3.882579, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000010 DAOPBQ010000000
VERSION     DAOPBQ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 17718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17718
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3287_length_17718_cov_3.882579"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..>237)
                     /locus_tag="PKW60_00795"
     CDS             complement(51..>237)
                     /locus_tag="PKW60_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97996.1"
                     /translation="AKAEAALWVLADRVTAPEDDGFAFGANVLFKDGHVEFIPFDAKA
                     PVTAGMAAFLDTLALLN"
     assembly_gap    238..247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<248..938)
                     /locus_tag="PKW60_00800"
     CDS             complement(<248..938)
                     /locus_tag="PKW60_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPJ97997.1"
                     /translation="MRNRGMSLLELLVILAILGLFAAILLPALAHARGSKARYDCAAN
                     LKQLGLASAMFAAEDAHGNYPALYAAHTPFMTETGESTGNSAFLVLPCLNPADLCPEY
                     LNDARAFVCPGVAAGNPAGFDAVADSSGRLVDELGLGLAARSYMYLGWAVDAIAPEAP
                     QEPLGILGGTSTLTAPAQLVLGVGPVLDEVRRSEDPARADLDIPVPGGLGTNGGTTIH
                     RLRKGVERFGVT"
     gene            complement(1107..2054)
                     /locus_tag="PKW60_00805"
     CDS             complement(1107..2054)
                     /locus_tag="PKW60_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ97998.1"
                     /translation="MTDEHRTPLWWASLTAEKVLGILFVLAAALKAWDLEAFAFQIRY
                     YHILTDPRWLMAAAIGSVAWETLLGAAMLVGLRFRGWTFIAVAATLVGFTGLVLYSWY
                     FYDLQDCGCFGSLVPLGPGATSVKNVGLFALAVLAWFGLRSGRAPIESAPWKHPLWKI
                     AAVLVSSVLVLVAGLFHAPFEHVSGNSGGQDGLSLAFNVEYEGQAFNTSQGEYFIAFL
                     SDTCDECRAETPLLNDMLDLPEMPVILAFMLVENDDSLEQFRELARFPTIRMDTLQWA
                     QYIHLAPPRFYLVRDGKELAHWDEHIPPLEEIVAARDAS"
     gene            complement(2068..3723)
                     /locus_tag="PKW60_00810"
     CDS             complement(2068..3723)
                     /locus_tag="PKW60_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPJ97999.1"
                     /translation="MKDVRYRPKLRQLPGLLLAALTVAAMAAEDPAPVPSPTFVMIPM
                     RDGVQLATDIYLPATGAPCPVVLARSVYGREFGPQWGPPWLDKGIAFAVQDCRGRGGS
                     EGTDLGFYADGWGEAGDGADTVAWLRAQPWCNGKIATFGGSALGIVQILAAPATPWIT
                     CQGIIVAPGSLYGNVTYQGGVFRKEMMEGWLEIQKLPHVLELWKEHPGYDAFWRHFDA
                     VSAADRTTAPAVHVGGWWDCFGQGTLEAFMSRQEEGALPGRGNQKLIMGPWLHGLRRE
                     LGDVTLAENFDAVDFNQYIQRFFRYWLLDEQNGIMEEPAVQYYTVGDLSDPNAPGNEW
                     RTASSWPPFPTRATPYYLNPDGMLQAEQVSERTGGLTFSYDPADPVPSLGGATLLHKP
                     GVYDQRPVSGRADVLKFATPPLAEPLEITGRVKVRLYVCTDARDTDFTAKLVDIYPDG
                     REILMLDGIQRLKFRNGFEMPDYLLPGEIAEVEIDLWSISLIFNAGHRIGLHVSSSNF
                     PRFERNPNTGKDFPAEDEPLAVASNTVFMGVRYPSALILPIAP"
     gene            complement(3777..6221)
                     /locus_tag="PKW60_00815"
     CDS             complement(3777..6221)
                     /locus_tag="PKW60_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPJ98000.1"
                     /translation="MNRPLVWVAGGILAGTMAGAGGYAPGVLIPLLVCAGGVACLYAF
                     SALPLSRALGVLLCFLGVGAFLHNVRHAAAPGDPLSRFLAMCEPGYCRIEGMVRDVPY
                     ASSPDGYARFLLDADRVVLGGQETVARGGVLVKWTDPGPLPWPGDRLTATGRWDLALA
                     RVNPGVQSWEDALRRSGIHTALSVRGAENIAILASAPWHSVKYWSARLRRAQAERFEH
                     AMPSPARAFALMIWLGDRSGLTPGDYTRYVDTGTSHILAISGIHISIVFVTATVFFET
                     LISARRRASALALLVVWLFALMAGGRAPALRAAIMFTMYVLADFVGRERDAPTALSIA
                     ALLFMLWRPDILFDAGFQLSFLSVASVLLFLPHFSELLERLPRPLRQPIAMPVAVLVL
                     PLPVAAYHFNSISLAAPLANLMVVPLLGAALWLCFATSVLSFVWMGAAALFGHALLPV
                     TLLIGWCIDAVRGLPYAHLDISRPSGLALLCYWLAAGALVAVLANLPWRRWAGVAAGV
                     LLVLAIVCWRPVPAPEAVVLDIGHADAIYIHTPGGDTLLVDGGDRSSYEDAGERVLLP
                     FLRARGIRRIDKVILTHPHRDHIGGLLSVFEKMQVGELLTGAELNTSTSETEEAQLAA
                     LCVQHAIPVRTVRKGDTIALAGASLHVLHPDPAWAGASNPNNRSVVCRLDWSGGSLLL
                     TGDIERPVETALCGPDCRSDVLKVPHHGSSTSSTGPFLDAVTPQYALVSTGPWRGRDP
                     VSPQVLERHRERGITVLRTDRHGGIRIPLRKQSFTVEAARLQRNYPVIGGGDPGASPI
                     APDAAIEYPAAKQALH"
     gene            complement(6278..6412)
                     /locus_tag="PKW60_00820"
     CDS             complement(6278..6412)
                     /locus_tag="PKW60_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98001.1"
                     /translation="MFQGGLGNLWGLLRSQIIVLEPTNVLSQIYNLLYNLLMLFAVLF
                     "
     gene            6613..7719
                     /locus_tag="PKW60_00825"
     CDS             6613..7719
                     /locus_tag="PKW60_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPJ98002.1"
                     /translation="MTAVKYVFKLVLAVALAVTLYPGCTPGGAPPTEHADSASPAPAA
                     PAASTGSATAGDPVAAIAEPPAMPMGYGSTIEVMAAIANGRIPLLDDNPPLPDGVKEL
                     AGLEYGNIDGVSLELDLALPANGEGPFPGLIFIHGGGWASGKRQVYHLYTYYFAQQGY
                     AAATISYRLSGQAKFPAAVQDVKCAVRWMRANAAAYHIDPDRIALIGGSAGGHLAMMA
                     GYTAGDPEFEGEGGNPGVNSAVQAVVNFYGPYDLTAEIARNSKEVLEFLGAAWDENPD
                     LYLQASPMHYLNAGDPPTLIFHGTIDDVVPIEQSERLAARLEELGIPCVFEPFEGWPH
                     TMDRAQQVNERCRWFMYHFFEAYLKPQPAAGAPQ"
     gene            7794..8816
                     /locus_tag="PKW60_00830"
     CDS             7794..8816
                     /locus_tag="PKW60_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HPJ98003.1"
                     /translation="MRVLVMGAGALGSAIGGFLANAGHAVTLVGRPAHIGAIERQGLR
                     ITGIWGEHRVRNLVPVTGTHALQRGDFDLILITVKSYDTRAAMAAIAPLLDPDTLVCS
                     YQNGLGNAEIIAETVGWSRTLGARAIYGVWLPEPGTAEVTVIANPTALGVYSSSAPAE
                     RVRAIAEAMDAAGVPTVYTDTIATVLWGKVAYNCALNPLSALLDVPYGALLDTQHTRA
                     IMRDVIRELYDVARAMDVRLEPSTPEAYCTLLFDELIPPTAKHYASMREDFRLKRRTE
                     IDALNGAICRYAESKDMTCPANTLLTRLVHAREYQMGVLDEMAVEGPNSDVFPRKAIP
                     YDFPWQ"
     gene            9006..9146
                     /locus_tag="PKW60_00835"
     CDS             9006..9146
                     /locus_tag="PKW60_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98004.1"
                     /translation="MRHIRTVTRARVCEAQAGMEVLRFIGALFSIFTALSRLFPAFAG
                     MK"
     gene            9309..10907
                     /locus_tag="PKW60_00840"
     CDS             9309..10907
                     /locus_tag="PKW60_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98005.1"
                     /translation="MSIFRRHRILRGVVFGFAGLFLVLAGFVAWRAKGPYRFYRLNVL
                     KTPATTGIQPGTLEIGVALRDITPDLSQYDPWVDVDGDNRFRPDKGDTYTDVNGNGRF
                     DAVWLAGFNNDRPAKGVHDPLWVRALAYRNNGITGVMVSIDSIGITNERFIAVRKSLP
                     DSLGLDLVTLASTHDHEAPDTMGIWSYKPLRGYFDKAYLEEVLSACRSAIVDAVGALE
                     PAEMTLASVRIEPEGFMDDSRLPHVYDDQICAAWFTRPGTRETIATLVSWGNHPETLG
                     GDNPLITSDYPHFLREGIEKGVDEPHGAPGLGGTCVFFTGNLGGLMTQLHTTVPHRDG
                     QQTFDKASFEKAQALGENVALKVLEALRGPDAISSANTEVAVAGRSFLVPLQGVFNWA
                     ILAGVIHPGWYWGKGRTEVHAIRIGDLAILTVPGELYPEIAEGGVECPDGADYPGEIL
                     ETPPLRKVMPGKVNMMFNLANDEVGYMLPRTQWDTKPPFAYEREKAQYGEENSGGPLV
                     GATYHRAALGLLRDLDSQMAAAAE"
     gene            complement(11025..11879)
                     /locus_tag="PKW60_00845"
     CDS             complement(11025..11879)
                     /locus_tag="PKW60_00845"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ98006.1"
                     /translation="MRRLRGIGLAISLVALWNSAASAELTLWLKYQYAADEACARGEF
                     NRAEHLYKAAERESAITLPADNFRLAGTLDGLGVVYMAQGRLDEADAVLQRALVMKEK
                     VLGPHTRELPKTILHIADLRYAQGKYDEVERLYRHALLLLVRDQTDIGDTRALNGLAR
                     YYRLQGDALKAESLLERARKIHVEARRRNHPAMADTLVELARLYTFEGRDDESLPLYD
                     KASAIRAEKLGMHHPQTAASFEELAQALHRQGRLQESEDLLERARAIRARHTEFEERY
                     ANAQAPLL"
     gene            12218..14689
                     /locus_tag="PKW60_00850"
     CDS             12218..14689
                     /locus_tag="PKW60_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98007.1"
                     /translation="MCLDVSAVRIFKPLAFVLMCCVVSCGQGKPAPAPTAFDSSAETA
                     VEAAAPAAPEVVNWSQARVYVASGNALLAKAAEMLQQEVAKRSGVQLPVSDGPASGTG
                     PAIVLGTAAELPASFSLPADVAVPDKAEGYAAQLDGGTLYLVGHDERGALYAAGHFIR
                     SAILSQGQIEVPETVSVSTAPLYPLRGHQLGYRNTANSYDAWTVDTYEQYVRDCILFG
                     ANAIEVIPSFNETKDQSPVMPVPQEQMNRKIAGMLHTYGIDVWLFLELGEDVRKPEEY
                     QQALETRDAMFAQYPAIDHVMVPGGDPGHTAPQDLMPWLADMAQVLRKHFPEAALWVS
                     NQGFTDDQNEVFFGYLQEHQPDWLTGVILGPWTQITLKEMRERTPERYPLRRYPDITH
                     SVRCQYPVPDWDRAFAQLLGRECANPRPRGTAHIHNLFAPLAAGFVSYSDGCHDDLNK
                     MIWTALAWNPDTDVKGLVRDYGRVFFGEAYADDIVEGLYMLETNWVGSALENESIDAT
                     LAHWLALEERAGADLSGNWRFQLYLLRAICDSYIRARLIAETAEEQEIYAALDGIRGA
                     GVDKAAAALHAKLSQTPPPEVRPELRQRIDELGMALRESIGMQLSVLPPYFASNPERG
                     AILDGIDLPLNNKLWILSELTRLQTLATENDQNALIERVLTWETPKPGALYDDLGCAW
                     KQPHLVKQTTWEADPGFVDGPQCEHFRGQDNNTREYTSNRLSWLDQAQTLFRAPLKMH
                     YEGLDTSKAYTLRVTYAGRFRPTMRLIANGDYEVHGPMPQPETWWPVDFPLPQELTAG
                     GVLDLEWQRIAGRGCQVAEVWLIPD"
     gene            complement(14837..16210)
                     /locus_tag="PKW60_00855"
     CDS             complement(14837..16210)
                     /locus_tag="PKW60_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPJ98008.1"
                     /translation="MTRTETKARMLVVDDEPGIRQVLETAFADRGFAVSTASNGDKAI
                     ELLTEQQFDVVITDLKMPGRDGMAVLRAAKEAAIDAGIIVITAYGTLETAVEAMRLGA
                     FDYIAKPFKLQEIELKVDKIVGRRRESGPPRDKAAELPEARKIMGDSRSTRQLLKMIE
                     KIGPSRSSVLITGPSGTGKELVARAIHEASPRRDLPFVALNCAALAPGVLESELFGHE
                     RGAFTGATGQRAGRFEQAHTGTLFLDEVAEIDPSIQTKLLRVLQEGEFERVGGQQTMK
                     VDVRIVAATNRDLREAIEKGDFRQDFFYRLNVFSLRTEPLKRRRDDIPSLVDVFLARF
                     SAETGKNVTGVEDEVMSFFMRYPWPGNIRELENMLERAVVLCEGSVITVEELPQELFF
                     IQEDDEPEPAEAPDHGSLVERTDKLESEMIQAALERFRWNKTKAAEHLGLKRTTLQYK
                     IKKYGLE"
     gene            complement(16429..16782)
                     /locus_tag="PKW60_00860"
     CDS             complement(16429..16782)
                     /locus_tag="PKW60_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98009.1"
                     /translation="MRQCHRCGAPWDGGGKRQPGSKDVCGACSAYLHCCLNCRFYEPG
                     AHNECYIPNTEWVGDKTACNFCDEFEFADRSTAPSDAQAGEQARNALDSLFGDSENRA
                     PDSERLDEFRKLFGD"
     gene            17008..17265
                     /locus_tag="PKW60_00865"
     CDS             17008..17265
                     /locus_tag="PKW60_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HPJ98010.1"
                     /translation="MIDQELIDILVCPENKTKVTLADQTLVDKANAAIEARTLTSRGG
                     EIIEEKIEGGLVREDRVYMYPIREGIPIMLIDEAIPLEQLT"
     gene            17418..>17718
                     /locus_tag="PKW60_00870"
     CDS             17418..>17718
                     /locus_tag="PKW60_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98011.1"
                     /translation="MTDFLRRHHDAIVTCRLTRAFEQELASISQGLATQSLTPAAAQQ
                     RAQRVRQLAEVLVQGKDRFDTSGGGSGVTFSQSSRARLEGAGDWSLTTRMELQDLQ"
BASE COUNT         3345 a         5473 c         5632 g         3258 t
ORIGIN      
        1 gcctcctcca ggggggatgg aggaggcgtt ttcgcgggtg gttgcgcggg ttaattcaag
       61 agtgcgagtg tgtcgagaaa agcggccatg ccggcagtca ccggcgcctt agcgtcaaag
      121 ggtatgaact cgacgtgtcc gtccttgaaa aggacattgg ccccgaaggc gaagccgtcg
      181 tcttccggcg cggtcacgcg gtcggcgagc acccagaggg cggcttcggc tttggccnnn
      241 nnnnnnncag tcacgccaaa ccgctcgacg cctttgcgca ggcggtggat cgtcgtgccg
      301 ccgttggtgc ccaggccacc gggcacggga atatcgaggt cggcgcgagc cgggtcttcg
      361 cttcgtctga cttcatccaa aaccgggccc acgcccagca ccaactgtgc gggcgccgtc
      421 agagtggaag ttccgcccag gatacccaga ggctcctgag gagcctcggg tgcgatggcg
      481 tccacagccc aacccagata catgtagctc cgcgctgcga gacccagccc gagctcatcc
      541 actaaacggc cactggaatc ggcgaccgca tcgaagccgg ccgggtttcc cgccgccacg
      601 ccggggcaca caaaagcgcg tgcatcgttg aggtattccg ggcagagatc ggcgggattc
      661 aggcacggca ggaccagaaa cgcgctgttt ccggtagatt cgccggtttc cgtcatgaat
      721 ggggtatgtg cggcgtacag ggcggggtag ttgccgtgcg cgtcttcggc agcgaacatc
      781 gcgcttgcga gacccaattg cttcaaattc gcagcacaat cgtagcgtgc tttggaaccc
      841 ctggcatggg cgagcgccgg cagcaggatt gcggcaaaca ggcccagaat agccaggata
      901 accaatagtt ccaggagtga catccctcgg tttcgcatcg tctcagtctc cagcgcacgc
      961 tgtccgaaga tatggtgcgt gcggttcctc atacccacct agacgaaacc ccgccccgtc
     1021 tattcacatc acggccgcga caggcgggca gcctccgaac acatttgttt tcttaggttt
     1081 agtgccgaat tgagcggccg ggtttgtcac gaagcgtcgc gggcggccac aatttcttcg
     1141 aggggcggga tgtgctcgtc ccagtgtgcg agttctttac cgtcgcgcac cagataaaag
     1201 cgcggggggg caaggtggat gtattgggcc cattgcaggg tatccattct gatggtggga
     1261 aaccgggcca gttcgcggaa ttgctcgaga gaatcgtcat tttcgacgag catgaacgcg
     1321 agaatcaccg gcatttcggg gagatcaagc atatcgttca gcaggggcgt ttcagcgcgg
     1381 cattcgtcgc aggtatcgct tagaaacgcg atgaagtact cgccctgcga ggtattaaag
     1441 gcttgcccct cgtattcaac gttaaatgcg agagagagcc catcctgccc cccggaattg
     1501 ccgctcacgt gctcgaacgg cgcgtggaag agcccggcaa ccaaaaccag cacgctggag
     1561 accaaaaccg ccgcgatctt ccacagggga tgcttccagg gcgcgctctc gatcggggcg
     1621 cggccggatc ggaggccaaa ccacgccagc acggcgagcg caaacagtcc gacgtttttc
     1681 acggaggtgg cgccggggcc gaggggcacg agcgacccga aacagccgca atcctggaga
     1741 tcgtaaaaat accaggagta cagcaccagc ccggtaaatc ccacgagggt cgcagcgacc
     1801 gcgatgaagg tccagccgcg aaaccggagc ccgaccagca tggcggcgcc cagcagggtt
     1861 tcccacgcca cggagccgat ggcggcggcc ataagccatc ggggatccgt gagtatatgg
     1921 tagtagcgaa tctggaacgc gaacgcctca agatcccatg ctttgagcgc tgccgccagg
     1981 acaaacagga tgcccaggac tttttccgcg gtcagactcg cccaccagag cggtgtacgg
     2041 tgttcgtcag tcatgtgctg catgatacta aggcgcgatt ggcaggatca acgcgctggg
     2101 gtagcgaacc cccataaaca ccgtgttcga ggcgaccgcc aacggctcgt cttcagcggg
     2161 gaagtctttg ccggtgttcg ggttgcgctc gaaacgaggg aagttgctgc tcgacacatg
     2221 cagtccgatg cgatggccgg cgttgaagat caggctgatg gaccacaaat cgatttcaac
     2281 ctccgcgatc tcccccggca ggaggtaatc cggcatttcg aagccgttgc ggaacttgag
     2341 gcgctggatg ccgtcgagca tgagaatctc ccgcccgtcc gggtagatat cgaccagttt
     2401 cgccgtgaaa tcggtgtctc tggcgtcagt acacacatag aggcgcacct tcacgcgccc
     2461 ggtgatctcg agcggttcgg caaggggcgg cgtggcgaat ttgaggacat ccgcccggcc
     2521 gctcacgggc cgctggtcgt ataccccggg tttgtggagg agcgtagcgc ctcccaggga
     2581 cggcacggga tcagccgggt cataggaaaa agtaaggccg cccgtgcgct cggacacctg
     2641 ctcggcctga agcatcccgt ccgggttcaa atagtaggga gtcgccctgg tgggaaacgg
     2701 cggccagctg ctggctgtcc gccattcatt gccgggcgcg ttcgggtctg acagatcgcc
     2761 cacggtgtag tactggactg cgggttcctc catgatgccg ttctgctcgt ccagaagcca
     2821 gtaccggaag aaccgctgaa tgtactggtt gaagtcgacg gcgtcgaagt tctccgcgag
     2881 ggtcacatcg ccgagttcgc gtctgagtcc atgcagccac ggccccatga tgagcttttg
     2941 attgcctcgg cccgggagtg cgccctcttc ctggcgcgac atgaacgcct ccagggtacc
     3001 ctggccgaag cagtcccacc atcctcccac gtgcacggcc ggcgcggttg tgcggtcggc
     3061 cgcgctcacg gcatcgaagt ggcgccaaaa cgcgtcgtac ccgggatgct ctttccacag
     3121 ctccagtacg tgcgggagtt tctggatctc cagccaaccc tccatcattt cttttcggaa
     3181 cacgcccccc tggtacgtga cgttgccgta cagtgagccg ggcgccacga taataccctg
     3241 gcaggtgatc cagggggtgg ccggcgccgc gaggatctgt acgatgccga gcgcggaacc
     3301 gccgaacgtg gcgatcttgc cattgcacca cggctgggcg cgcagccacg cgacggtgtc
     3361 ggcgccgtca ccggcctcgc cccatccatc ggcatagaac cccagatcgg tcccctcgct
     3421 gccgccacgg ccgcggcagt cctggacagc gaacgcgatg cccttgtcga gccacggagg
     3481 cccccactga gggccgaatt cgcggccgta cacactccgg gccagcacga cagggcaggg
     3541 agcgccggtt gcgggaagat agatgtcggt tgcgagctgg acgccgtcgc gcatgggtat
     3601 catgacaaat gtgggcgatg gaactggggc cgggtcctcc gcggccatgg cggcgacggt
     3661 caaggcagcg agcagcaatc ccggcagttg ccggagtttt gggcggtatc gcacgtcttt
     3721 catggcgttc ctctcagatg gcaaaacggc ggccctgcgt gtacggccag ggcgtctcag
     3781 tgcagcgcct gtttcgcggc aggatactcg atggccgcgt ctggcgcaat cggagacgcg
     3841 cccgggtcac cgccgccaat gacggggtag ttgcgctgca agcgcgcggc ttccacggta
     3901 aaggattgtt tgcggagcgg aatgcgaatg ccgccgtgac gatctgtgcg caaaaccgtg
     3961 atcccccgtt cccggtgccg ctcgagcacc tgcggtgaaa cggggtccct gccgcgccag
     4021 ggtcccgtcg ataccagggc gtactgcggc gtgacggcgt cgagaaaggg cccggtagaa
     4081 gaggtgctcg acccgtggtg cggcactttc agcacatcgc tccggcagtc cggtccgcat
     4141 aaggccgttt ctacgggcct ttcaatgtcg cccgtgagca gcaggctgcc gccgctccag
     4201 tcgagccggc acaccaccga ccggttgttc ggattggatg cgccggccca ggccgggtcg
     4261 ggatgcagca cgtggagcga ggcccccgcc agggcgatgg tatcgccttt gcgcaccgtg
     4321 cgcacgggaa tcgcatgttg gacgcaaagc gccgcgagct gagcctcttc cgtctcggac
     4381 gtggaggtgt tcaattccgc cccggtcaac aattctccga cctgcatctt ctcaaagacg
     4441 ctgagcaggc ctccgatgtg gtcgcgatgc ggatgcgtca gaatgacttt atcgatacgc
     4501 cggatgccgc gcgcgcggag gaagggcagc aggacccgtt cacctgcatc ttcataggag
     4561 gagcggtcgc cgccatcgac caggagtgta tcgccgccgg gcgtgtggat gtagatggcg
     4621 tcggcatggc caatatccag gacgaccgct tccggggcgg gcaccggccg ccagcatacg
     4681 atcgccagaa ccagcagcac gccggccgct acacctgccc agcggcgcca ggggagattc
     4741 gcgaggaccg cgaccagagc gcccgcggcg agccagtaac acaacagggc caggccagag
     4801 gggcgcgata tatcgagatg ggcgtatggc aatccgcgca cggcgtcgat gcaccatcca
     4861 atcagcagcg tcacgggcag cagggcatgg ccaaacaggg ccgccgcacc catccagaca
     4921 aacgacagga cgctcgtggc gaagcacagc cacagtgccg ctcccaggag cggcaccacc
     4981 atcaggttgg ccagcggcgc cgcaagagag atgctgttga agtggtatgc cgccacgggg
     5041 agcggcagca ccagcaccgc caccggcatc gcgatcggct ggcgaagagg gcgaggaagc
     5101 cgttccagaa gttcggagaa atgcggaaga aacaggagga ccgacgcgac acttaagaac
     5161 gacagctgga accccgcatc aaacaggata tccggccgcc acagcatgaa caggagcgct
     5221 gcgatgctca gggccgttgg tgcatcgcgt tcccggccga cgaaatccgc cagcacgtac
     5281 atcgtgaaca taatggccgc tcgcaacgcc ggggcgcgcc cgccggccat cagggcaaag
     5341 agccaaacca ccagcagggc cagcgccgac gcccgccgcc gggcggagat cagggtttcg
     5401 aaaaacacgg tcgccgtgac aaatacgatg gagatatgaa tgcccgagat ggcaaggata
     5461 tgcgacgtgc cggtgtcaac ataccgggta tagtcgccgg gcgtcaggcc gcttcggtcg
     5521 cccagccaga tcatcagtgc aaaggcccgc gccggagaag gcatggcgtg ctcaaaccgc
     5581 tcggcctgag cccgccgcag ccgggccgac cagtatttca cggagtgcca gggagcggac
     5641 gcgagaatcg cgatgttttc tgctccgcgc accgagagcg cggtgtgaat gccgctgcgg
     5701 cggagcgcat cttcccagct ctgcacgccc ggattgacgc gggccagggc gagatcccag
     5761 cgcccggttg ccgttaaacg atcgcccggc cacggcagcg ggccggggtc cgtccatttg
     5821 acgagcacgc cgcccctggc gacggtttcc tgaccgccga gcaccacccg gtcggcatcc
     5881 agcaaaaacc gggcgtatcc atcgggcgaa ctggcgtagg gcacatcgcg aaccatgcct
     5941 tcaatacggc agtaaccggg ttcgcacatc gcgagaaagc ggctgagcgg atcgccaggc
     6001 gcagcggcgt ggcgcacgtt atgcaaaaat gctcccacgc ccagaaaaca aagaaggact
     6061 cccaatgcac gagacagcgg cagcgcggaa aaggcataca ggcaggccac accccccgcg
     6121 cataccagca gcggaatcag cacgccgggc gcatatcccc ccgcgcccgc catcgtcccc
     6181 gccaagatcc ctccggccac ccataccagc ggccggttca ctgtccttct ctcctttcag
     6241 tcatggcgca cccccgcatg ccggccccgt ccggctgtca gaagagcacc gcgaacagca
     6301 tcaggagatt gtataagagg ttgtagatct gcgacagcac gtttgtgggt tccagcacga
     6361 taatctgcga cctcaacagg ccccacaaat tgcccaaacc accctggaac atgttctcgt
     6421 ctccttcatg cgcggccccg cgcgcgatcg atatcagata accccatcag actagccttc
     6481 tcgccgcatg aactcaagaa cgggccgcgc cggaccgtgg tgcatcggaa atttgtgcgc
     6541 cgttcgtgat attctggcga tcaatctcta tgcgccgttc ctgttggcta atctaccgaa
     6601 ggaggtctcc ccatgactgc cgtgaaatac gttttcaagc ttgtgcttgc ggtcgcgctt
     6661 gcggttacgc tgtaccccgg atgcacgccc ggaggcgcgc cgccaaccga acacgcagat
     6721 tcggcgagcc ctgcgccggc cgccccggcg gcatcaaccg gatctgccac agccggcgat
     6781 ccagtcgcgg cgatagcgga gccgccggcc atgcccatgg gatacggctc gactatcgag
     6841 gtcatggcgg cgattgcgaa tggccgcatc ccactcctgg acgataatcc gccgctgccg
     6901 gacggtgtga aggaactcgc cggccttgag tacggcaaca tcgacggagt atccctggaa
     6961 ctcgacctgg ccttgcccgc caatggagag gggccgtttc ccggattgat tttcatccac
     7021 gggggcggct gggccagcgg aaagcggcag gtctaccatc tctacaccta ctatttcgcc
     7081 caacagggct atgcggccgc cacgatctcc taccggctct ccggtcaggc caagtttccc
     7141 gcagccgttc aggatgtgaa atgtgcggtc cggtggatgc gcgcgaatgc cgccgcctac
     7201 cacatcgacc cggatcgtat cgcgctgatc ggcgggtcgg cgggcggcca tctcgcgatg
     7261 atggcaggct acaccgccgg tgatccggaa tttgaaggcg agggtggaaa tcccggcgtc
     7321 aacagtgccg tgcaggccgt cgtcaatttc tacggaccgt atgatctcac cgccgaaatt
     7381 gccaggaact cgaaggaggt cctggagttt ctgggagcgg cctgggacga aaaccccgat
     7441 ctgtatctcc aggcttcgcc catgcactac ctgaacgcag gtgatccgcc caccctgatt
     7501 tttcacggaa ccatcgatga cgtggtgccg attgagcaat ctgaacgcct cgcggcgcgc
     7561 ctcgaagaac tgggcattcc ctgtgtgttc gaacccttcg agggatggcc tcataccatg
     7621 gatcgcgccc agcaggtcaa cgaacggtgc cgctggttca tgtaccactt ctttgaggcc
     7681 tatctgaagc ctcaaccggc cgcgggagcc ccgcaatgag gcaaaatggc cggcattctg
     7741 aacagtgcgg gcgcattctc gatttcagca cgggccatcc gtaggagcgt atcatgcggg
     7801 tactggtaat gggcgccggg gcgttgggtt ccgcgatagg cggatttctg gccaacgctg
     7861 gacatgccgt cacgctggtc ggacgccctg cccacatcgg cgctatcgaa cggcagggac
     7921 tgcgcatcac gggcatctgg ggcgagcatc gggtccgtaa tcttgtgccc gtgacgggaa
     7981 cccatgcgct ccagcggggc gacttcgatt tgatcctcat caccgttaaa tcatacgaca
     8041 cgcgggcggc gatggcagcc attgcgcctc tcctggaccc cgataccctc gtctgttcgt
     8101 atcaaaacgg actgggcaac gcggagatca tcgccgaaac cgtcgggtgg tccaggacgc
     8161 tgggagcacg ggccatctac ggggtgtggc tgcccgagcc cggcacggcc gaagtcacgg
     8221 tgatcgcgaa tcccactgca ctgggcgtgt attcctccag cgcgccggcc gaacgggtgc
     8281 gcgccattgc ggaagccatg gacgccgccg gagtgcctac cgtctacacc gacacgatcg
     8341 ccaccgtgct gtggggcaaa gtggcctaca actgtgccct caacccgttg tccgcgctgc
     8401 tggacgtgcc gtacggggcg ctattggaca cgcaacacac ccgcgcgatc atgcgcgacg
     8461 tcatccggga attatatgat gtggcccggg ccatggatgt tcgcctggaa cccagtacgc
     8521 ccgaagcgta ctgcaccctc ttgtttgatg aactcattcc tccgacggcg aaacactacg
     8581 ccagcatgcg agaggatttc cgcttgaaac gccgcaccga gatcgacgcc ttaaacggtg
     8641 caatctgccg ctatgccgaa agcaaagaca tgacctgccc ggccaacacg ctcctcaccc
     8701 gtctcgtcca tgcccgcgaa taccagatgg gcgtccttga cgaaatggcc gtggaagggc
     8761 cgaacagcga tgtatttccc cgcaaggcaa taccgtatga ttttccctgg cagtagcagt
     8821 ccctggttct gcaaatgcag ctttttcttt cgaaacgccc caaattcgag tgtgagccgg
     8881 ctgctccaga gacaattcgt gctgttgact cgccggggca ttcacattat gctgatgcgt
     8941 gccgttgcgc agtggacagg tgcaggcagg cgggcagcct ttaacggaga aggaaaggga
     9001 ttcatatgag acacattcga accgttacgc gggcacgtgt gtgcgaagct caggctggaa
     9061 tggaagtcct gcgctttatc ggggcgctct tttcgatttt tacggcgttg tccaggcttt
     9121 tccccgcttt cgccggcatg aaatagaccg cttcggagca gtgtgcgaca cgggccgtcc
     9181 aaacagggcg gcctgtgttt ttcccctcgt catgcgcggt ataccctcgt tattttcatc
     9241 ggttaccatg ttgtattgca tcgctctggc tcgcgactga agtgtacgcg ggatgtggag
     9301 gcgcaggaat gagcatcttc aggcggcatc gcattcttcg tggggtcgtg ttcggctttg
     9361 ccggcctgtt tctggtgttg gccggctttg tcgcctggcg ggccaaaggc ccgtaccggt
     9421 tttaccggct gaatgtgctc aagacccctg ccaccacggg catccagccc ggcacgcttg
     9481 agatcggcgt tgctttgcgc gatatcaccc cggatctctc ccaatatgac ccctgggttg
     9541 atgtcgatgg cgacaaccgg ttccggcccg acaaaggcga tacctatacc gacgtcaacg
     9601 gcaacgggcg ctttgatgcg gtctggctgg ccgggttcaa caatgaccgc ccggccaaag
     9661 gcgtgcacga tccgctgtgg gtgcgggccc tggcgtaccg aaacaacggc atcaccggcg
     9721 tgatggtctc catcgacagc atcgggatca cgaacgagcg gttcattgcc gtccggaaat
     9781 cgctgccgga ttccctcggc ctggatttgg tcaccctggc ctcgacgcat gaccacgagg
     9841 cgccggatac catgggtatc tggagctaca agccgctccg gggctatttt gacaaggcct
     9901 atctggaaga agtgctctcc gcctgccgct cggccatcgt ggacgccgtg ggtgcgcttg
     9961 aacccgccga aatgacactg gcttccgtgc ggatagaacc cgaaggcttc atggacgaca
    10021 gccgcctgcc gcatgtgtat gacgaccaga tctgcgcggc ctggtttacg cggcccggca
    10081 cccgggagac cattgccacg ctggtctcgt ggggaaatca tcccgagact ctcgggggtg
    10141 acaatccgct gatcacctcc gattatccgc attttttgcg ggaaggcatc gaaaaagggg
    10201 tcgatgagcc tcacggcgcg cccgggctgg gcggcacctg cgttttcttc acgggcaatc
    10261 tgggcggact gatgacccag ttgcacacga cggtgcccca ccgcgatggc cagcaaacgt
    10321 tcgacaaagc gtcatttgaa aaggcgcagg ccctgggcga aaacgtcgcc ctcaaggtgc
    10381 tggaagccct gcgggggcct gacgcaattt cgtcggcaaa caccgaagtg gccgtggcgg
    10441 gccgctcctt tctcgttcct ctgcagggcg tgttcaactg ggccattctt gcgggagtca
    10501 ttcatcccgg ctggtactgg ggtaaaggac gcaccgaagt gcatgcgatc cggattggcg
    10561 acctggccat cctcaccgtt cccggcgagt tgtatccgga aattgccgaa ggcggcgtcg
    10621 aatgcccgga cggcgcggat tatcccggtg agatactgga aacgcctccg ctccggaaag
    10681 tcatgcccgg caaggtcaat atgatgttca acctcgccaa tgacgaggtc ggttacatgc
    10741 tgcccaggac ccagtgggac accaagcctc cattcgcgta cgagcgtgag aaagcccagt
    10801 acggggaaga gaactcgggc gggccgcttg taggcgccac ctatcaccgg gcggccctcg
    10861 gcctgttgcg cgatctcgac agccagatgg cggcggcggc tgaatagaac gccctgggca
    10921 gtcctgtatc gattcctatc cgcggcagat ctgcccggct ggttccccgc gcaaaaacat
    10981 acgaaactgg atgccggcgc cgtgttttta gctcttggcg agaactacag caaaggcgcc
    11041 tgagcgtttg cgtaacgctc ttcgaactcg gtgtgccggg cccggatggc ccgggcgcgt
    11101 tcaagcaagt cttcggactc ctggagccgg ccctggcggt gaagcgcctg ggcgagctcc
    11161 tcgaagcttg ccgcggtctg cgggtggtgc atgccgagtt tttcggcgcg gatggcgctg
    11221 gccttgtcgt acagcggcag agattcgtcg tcacgccctt caaacgtgta aagccgggcg
    11281 agttcgacca gggtgtcggc catggcggga tggttgcggc ggcgggcttc cacgtggatt
    11341 ttccgcgcgc gctccaggag cgactccgct ttcagggcat cgccttggag gcggtaatac
    11401 cgggccaggc cgttgagcgc gcgggtgtca ccgatatcgg tctgatcgcg caccaggagc
    11461 agcagcgcat ggcggtacag gcgctccact tcatcgtatt ttccctgggc ataccgcagg
    11521 tcggcgatat ggaggatggt cttcgggagt tcgcgggtat gggggcccag gactttttct
    11581 ttcatgacca gagcgcgctg aagcacggca tcggcctcgt ccagccggcc ttgcgccata
    11641 tagaccacgc ccagtccgtc gagggtaccg gcgaggcgga aattgtcggc gggcagggta
    11701 atggcagact cccgttcggc cgccttgtac aggtgttcgg cacggttgaa ctcaccgcgg
    11761 gcacaggcct cgtctgcggc atactgatac ttgagccaca aggtcagctc agccgatgcg
    11821 gcggagttcc acagcgcgac aagcgatatc gccagtccaa tgccgcgaag tcttcgcatg
    11881 gcggtctccc ccctttccgc acgccgggcg tgcgtttcgc ctcttggtgt catgtgtcag
    11941 aattgtagca catgctgccg gttgcacaca gagcgcgcac cgcgatgcac tctgcgcatc
    12001 gcgagggaag aacggagcgt tgattcaaat gcaatccgcg gggatcgcat tttcgccgcg
    12061 gcggcgcgac cgtgtatcga aggaacaatt gcgttttgct cttgtccgct ggctggtgct
    12121 atagtgtcgc attccccggt tgccgattcg gtgtggtggt ggggcaagag cggcaatgcg
    12181 cacggggaac ataacccgtc aagaggaggt gcgcacaatg tgtctcgatg tttcggcggt
    12241 gagaattttt aaacccctgg cgtttgtgct gatgtgctgc gtggtttcgt gtggtcaggg
    12301 gaagcctgca cccgcgccaa ccgcatttga ctcatcggcc gaaaccgctg tagaggcagc
    12361 tgcacccgcg gcgccggagg tggtgaactg gtcgcaggca cgcgtctatg tcgcgagcgg
    12421 caacgccctg ctcgcgaagg ccgcagagat gcttcagcag gaggtcgcga aacggagcgg
    12481 cgtccagctc cccgtgagcg acggcccggc gtccgggaca ggcccggcga ttgtgctggg
    12541 gaccgccgca gaattgccgg cgtcgttttc gctgccggcg gacgtggcgg tgccggacaa
    12601 ggcagagggg tatgcggcgc agctcgatgg cggcacgctg tatctggtgg ggcacgatga
    12661 gcgtggcgcg ctgtatgcgg ccggccattt catccgctcg gccatcctgt cgcaggggca
    12721 gattgaagtc ccggagacgg tgtcggtctc gacggcgccc ttgtacccgt tgcgggggca
    12781 tcagctcggt taccgcaaca ccgcgaacag ttacgatgcg tggacagtgg acacgtatga
    12841 gcagtacgtg cgggattgca ttcttttcgg cgcgaatgca atagaggtaa tcccgagctt
    12901 taatgaaacc aaagatcaga gcccggtgat gccggtgcct caggagcaga tgaaccggaa
    12961 gatcgcgggg atgctgcaca catacggcat cgatgtgtgg ctgtttctgg aactgggcga
    13021 agatgtgcgc aagccggagg aataccagca ggcactggag acacgcgacg cgatgtttgc
    13081 ccagtatccg gcgatcgatc acgtgatggt gcccggcggc gacccggggc atacggcgcc
    13141 gcaggatctc atgccgtggt tggccgacat ggcccaggtg ctgcggaaac attttccaga
    13201 ggcggcgctc tgggtgtcca atcagggatt tacggatgac cagaacgagg tctttttcgg
    13261 gtaccttcaa gaacatcagc cggactggct cacgggcgtg attttagggc cgtggacaca
    13321 aatcaccttg aaggaaatgc gggaacgcac acccgaacgc tatccgctgc gccggtaccc
    13381 ggacatcacg cacagcgtgc gctgccagta tccggttcca gactgggatc gggcgtttgc
    13441 tcagctgctc ggtcgtgaat gtgcaaaccc gcggccccgc ggcacggcgc acatccacaa
    13501 cttgtttgcc ccgctggcgg caggatttgt gtcgtattcc gatggttgcc atgacgatct
    13561 gaacaagatg atctggacag ccctggcctg gaatcccgac acggatgtga agggtctcgt
    13621 gcgggactac ggccgggtat ttttcggcga ggcctatgcc gatgacatcg ttgaaggcct
    13681 gtacatgctg gaaacgaact gggtgggctc ggcgctggaa aacgagagca ttgacgcgac
    13741 gctggcacac tggctggccc tggaggagcg cgcgggcgcg gatctgtcag ggaactggcg
    13801 ttttcagctg tacttgttgc gcgcgatctg cgacagctat atccgtgcgc gcctgatcgc
    13861 agagacggcg gaagagcagg aaatctacgc cgcgctggat gggatccggg gcgcgggcgt
    13921 ggataaagcc gcggccgcgc tgcatgcaaa actgagccag acgccgccgc cggaggtgcg
    13981 cccggaattg cgccagcgca ttgacgagct tgggatggcg ttgcgcgaga gcatcgggat
    14041 gcaactgtcg gtgctgccgc cgtatttcgc gtcgaatccg gagcgcggcg ccatcctgga
    14101 cgggatcgac ctgccgctca acaacaagct gtggatcctg agcgagctca cgcggcttca
    14161 gacactggcc accgaaaatg accagaacgc gctgatcgaa cgcgtcctca cctgggagac
    14221 cccgaaaccg ggtgcgctgt acgacgacct gggctgcgcg tggaagcagc cccacctggt
    14281 gaaacagacg acctgggagg cggatccggg ctttgtcgat gggccgcagt gcgagcattt
    14341 ccgaggccag gacaacaata cccgggagta caccagcaat cggctgtcgt ggctggatca
    14401 ggcccagacg ctgttccgcg cgccgctcaa gatgcactac gaggggctgg atacgtcaaa
    14461 ggcgtacacg ttgcgggtga cgtacgcggg ccggttccgt ccgacgatgc gcctgatcgc
    14521 gaacggcgac tatgaagtgc atggccccat gccgcagccc gagacgtggt ggccggtgga
    14581 tttcccgctg ccgcaggaat tgacggcggg cggcgtgctg gatctcgaat ggcagcgcat
    14641 cgcaggccgt gggtgccagg ttgcggaagt gtggctgatc ccggattgag ccgggcagcg
    14701 tgccggatga tcgtgtttat ggcggcgaag ttctcttcgc cgccattttc ttatgaagcg
    14761 cctgaccggt ccgcgattcc gggcgcctgc aaagctcatt gcatgaagcg gggatcgaag
    14821 ccgatcgcgc gccgggttat tccagcccgt actttttgat cttgtactgg agagtggtgc
    14881 gcttcaggcc gaggtgttcc gcggccttgg ttttgttcca gcggaagcgt tccagggcgg
    14941 cctgaatcat ctcgctttcg agcttgtctg tgcgttccac gagcgacccg tgatccggcg
    15001 cctcggcggg ctccggctca tcgtcctcct ggatgaagaa cagttcctgg ggaagctcct
    15061 cgacggtaat cacgctgccc tcgcacaaga ccacggcccg ctcaagcata ttctccagct
    15121 cgcggatatt gcccggccag gggtagcgca tgaaaaaact catgacttcg tcttccacgc
    15181 cagtcacgtt tttgccggtc tccgcgctga agcgcgccag aaacacgtcg accagcgagg
    15241 gaatatcgtc gcgccggcgt ttcagtggct cggtacgcag agaaaacacg ttcaggcggt
    15301 aaaagaagtc ctgacggaaa tcgcccttct caatggcttc gcgcaggtcg cggttggtgg
    15361 ccgccacgat ccgtacatcg actttcatgg tttgctggcc gcctacccgt tcgaattctc
    15421 cctcctgcag cacgcgcagc agcttggtct ggatggaggg gtcaatctcg gccacttcgt
    15481 cgagaaagag tgtgccggtg tgggcctgtt cgaaacggcc cgcgcgctgg cccgtggcgc
    15541 ccgtaaatgc gccgcgctca tggccgaaca actcgctctc gagcacgccc ggcgccaggg
    15601 cggcgcagtt cagcgccaca aaaggcaggt cacggcgcgg actcgcctcg tggatggccc
    15661 gggccaccag ttctttgccg gttccgctcg ggcccgtgat gagcacggaa ctccggctcg
    15721 gcccgatctt ctcaatcatc ttcagcaatt ggcgggtcga acggctgtcg cccatgatct
    15781 tgcgtgcttc gggcagttcg gccgctttgt cccgtggcgg accgctttct ctgcgccgtc
    15841 cgacaatttt gtcgactttc agctcgattt cctggagctt gaacggtttt gcgatgtagt
    15901 caaacgcgcc caggcgcatt gcttcgactg cggtttccag ggtgccatag gccgtgatca
    15961 cgattatgcc cgcatcgatc gccgcttcct tcgcggcgcg caataccgcc attccgtcgc
    16021 ggcccggcat cttcagatcc gtgatcacca catcgaactg ctgctctgtg agcagttcga
    16081 tggccttgtc gccgtttgag gcggtagaca cggcaaaacc ccggtccgca aaggccgttt
    16141 cgaggacttg ccggataccc ggttcgtcgt cgacgaccag catacgcgct tttgtttccg
    16201 ttcgggtcac ctcaggctcc tgggcttcag gacgttcacg gcctcctgac gaatgccggt
    16261 atgtctcccc gccccgtgca acaactcacg gcccgccccg ccgcatcacc cggccaaacc
    16321 gctttgttgc gacgtgactt ccatactttg tatcacttgc cgctggccgc gtcaaataat
    16381 cggccgggag tcacagtaaa atggctatgg taattcgaat ttttgcgatc aatcgccgaa
    16441 aagtttacgg aattcgtcca ggcgttctga atctggcgcg cggttttcgc tgtctccaaa
    16501 gaggctgtcc agcgcgttgc gcgcctgctc gcccgcctgg gcgtcagacg gggcggtgga
    16561 gcgatcggca aactcgaatt cgtcgcagaa gttgcaggca gtcttatcgc cgacccattc
    16621 ggtgttggga atatagcatt cattatgcgc ccccggctca taaaagcggc agttcaggca
    16681 gcaatgcagg tacgctgagc aggcgccgca gacgtccttc gagccgggct gtcttttgcc
    16741 gccgccgtcc cacggggcgc cgcagcgatg gcattgtctc atgaatcgaa ctcccgcttt
    16801 gcacgtggta atcccgcaca ggacccggtt tgtggccagc ctaaccgcct cgccgcgctg
    16861 agtcaaccgg tgcacgccac ccggcgcgtt aggcggaccc tgtgagcgcg ggccgttccg
    16921 tttcgaagag cgggtttgcc ttggagttaa gcgccggatg ggctagaata accgcagcac
    16981 ggcggtacag agaaggagct ccattcgatg atagaccagg aactgatcga catattggtg
    17041 tgtccggaaa ataagacgaa agtgaccctg gccgaccaaa ccctcgtcga caaggccaac
    17101 gccgccatcg aagcccgtac gctcacgtcg cgcggcggtg aaatcatcga agagaaaatc
    17161 gaaggcggac tggtgcggga agatcgggtg tatatgtacc ccattcgcga gggcatcccg
    17221 atcatgctga tcgatgaggc gatcccgctg gaacagctca cctgaaccga cgcggggaag
    17281 cgccggtcca ctggggccgc ctgcgcctcc gccgggtctg cgtcctgggg gcgctgattc
    17341 cggcctgctg ggaccatgtc tgctccccgt cgcttgccga gctggattgc ctcacctatc
    17401 cgcttcgccc cgaagcgttg accgactttc tgcgccgtca ccatgacgct attgttacct
    17461 gccgcctcac ccgcgccttc gagcaggagc tcgcgagcat ctcgcagggc ctcgccacgc
    17521 agtcgctcac cccggccgcg gcccagcagc gcgcccagcg cgtgcggcag ctcgccgagg
    17581 tgctggtgca gggcaaggac cgcttcgaca ccagcggcgg cggcagcggc gtgaccttca
    17641 gccagtcgtc gcgcgcccgc ctcgagggcg caggcgactg gagcctcacc acccgcatgg
    17701 agctccagga cctccagc
//
LOCUS       DAOPBQ010000011        17333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_3362_length_17333_cov_4.332156, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000011 DAOPBQ010000000
VERSION     DAOPBQ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 17333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17333
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3362_length_17333_cov_4.332156"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..1166)
                     /locus_tag="PKW60_00875"
     CDS             complement(264..1166)
                     /locus_tag="PKW60_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98012.1"
                     /translation="MPGAIRIYDTTLSQRFTWTGPVVSVDQATAIARALEQAGVSTIE
                     AGYPAQSSSQRAIVKAVAKCISTSEVAVLAACDPAEIAAAIGAVRHAAKPLVHVYLPS
                     GPARSGGKRVEWCRAIERAVTAVRAAGIEVQFSIGGMETMERPFRRQCIGVAVSAGAT
                     RIAVSDSTGAMKTAAYVEMVRDIVQFVESRAIVSASCHNVSGRAVHNALAAVEAGARQ
                     VETTAGEIGTEAGCTPLEGFVDQARGRFDVVAVDRRAAASIAALMPANSAPAPAQTGS
                     AAREMAAVAAPCPDWYCRVEVAAL"
     gene            1675..2127
                     /gene="mscL"
                     /locus_tag="PKW60_00880"
     CDS             1675..2127
                     /gene="mscL"
                     /locus_tag="PKW60_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPJ98013.1"
                     /translation="MLKEFKEFALRGNVVDMAVGIVIGGAFGTIVKSLVDDVLMPPIG
                     WVVGGVDFSNFFLVIKDGSTPGPYFSLGAAKTAGAVTLNYGLFANSVISFLIVAFALF
                     MVVKAMNKLHRKEEPAAEPATKECPYCLSSIPLKASRCPHCTSAIEAI"
     gene            2565..4949
                     /locus_tag="PKW60_00885"
     CDS             2565..4949
                     /locus_tag="PKW60_00885"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HPJ98014.1"
                     /translation="MLGVLCAGAAGAMEAGAAKVEITPELGTPLAGSYQRMGRGATAV
                     HDPFYVRALYLEDGDTRVFLVSADLCVISPDLRQGVLERPLHGVPRENVILTATHTMN
                     GPGGMVYSPAFRVFTGRYMPELVESMADRFAEAMAEAYGNRKRATIGFGTTEPQALSR
                     NAVTKDGLVDAQLGVLRVNDSDGKPLAIVGNFGARCIGVNIPGPEMLTLSADVPGRFC
                     EELEKLAEGSVAMFLQGACGDAEFGNPSGATGWARAETAGRALAQLAFETAQTIKSND
                     QPLAVSFANVEFPPTLADAYLPSAAPLATLEIGSLAMTFIPGAPGGAISKQLRSFCLT
                     LGYEAQFTVGVANDYRFFFLSGPEFSAPGPESLLSFYGPGMGDWLLTNFSGLLSKGAA
                     GEPAVQEDVPEVQAESGIQRIILKGTPHQLGYARGAAFRETLQAQFEERYVKPAVSGT
                     LAPPEPFVASLPVCVRPYLALSNVTLPWMAMRSKTMLARLPEGQFEELAGLAAGADMP
                     FDAAWLLQCAPLMETGDVRGGAPPESFGVVFAAVGLKAGADDILVGHNLDSPGPFVPV
                     VYDVRPAQGLRYFLIGSPARCGGFSGINEEGVVVCVERNELLGSPAQSGPPVELVAAH
                     ILSKARNAWAALDMIETFPHLKGYHILLGDPVSGGNGQPPARVVTLDASPRLRAPTEG
                     TLLGANPEAEQLSDAARDRYLRLAALIAEERNLNPSEIEEILLDSQTSVSETSGVFNS
                     NTRYSAVFEPKARRMRVAAPGPDGSPGTYVRFEFESGAGSKGAARARETGGSVR"
     gene            4946..6547
                     /locus_tag="PKW60_00890"
     CDS             4946..6547
                     /locus_tag="PKW60_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98015.1"
                     /translation="MSDALRTFQGPVRGRWSSTRRGRRRRGIPFKRFFFVVLLLLAGA
                     GYWISRDTHAVHSLIPSTQKYSVVLRDILRNRAELAGSAIWDALPPESGSGKITQVLR
                     GELGLPEWMARNIIVDDCYISGNDLRDFNDVLFVTKMTRIGTLIEQLHWVTSRIGRDT
                     AGGLGLRHFPAAGVYYAVRGRILLISPSRDALVNALTLQDDGRLTEAAFDQDFEKIAG
                     EDIGGTVTFALDDPLGEILEDIRFCLRIDQTQAHLNWNARLRAEQHARLAPLLSGVSP
                     GTLLIPPPGLVAVSGNLNKPLGEVWRALDGSFNCGGGPGLVSETGEIPEENWPKEGPP
                     SLAQLLEMILGPLGPGWRIALQGVDLNEWFPVPIFAGTFDLPQGTASDFLAQLPPPPG
                     DAMVWESYPRYDAAAGMLRVPMIAGPSLEPTAAPYGESLLVCSSASLAEDILAAEPAL
                     RPLEQPGNFYIRLQPSACARAINETLELLVRGNILKEQAVRDFHAAFDAWLYGAARVS
                     EAAGLLAYEDGGLTADLVVICAPAL"
     gene            6605..7450
                     /locus_tag="PKW60_00895"
     CDS             6605..7450
                     /locus_tag="PKW60_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPJ98016.1"
                     /translation="MPDHIKEENPDGAASQETEPVSTGGEGVIVDEATDGILRLHLDM
                     FEGPFEVLLYLIKVQEIDIVDIPIVTITEQYLGFLELMREENLDVTGEFLLMAATLIQ
                     IKSRMLLPPDHDPEDDEEQIEEEDPRIELVEKLIEYRRYRDVAARLEYLEGERARWFM
                     RNSKPEIEPEEDEEEELLEVTLFDLIEAFRGVVRFFSDDLFHSVKSEESSVDEKIEYI
                     ENSLKEDGSLAWADLLGQCRSRVELVCCFLAILELCRMGRLRAHQHGPFGEIRLVAAG
                     REPVA"
     gene            7447..7827
                     /locus_tag="PKW60_00900"
     CDS             7447..7827
                     /locus_tag="PKW60_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPJ98017.1"
                     /translation="MNWRPWHWTRSLGQRGEDLAARFLRRQGYSILGRNVSFGRYEID
                     IIAEAGDTVAFVEVKTRCSDDLVEPEENVGYIKQRHIRRAAWQYIDKRDDPNLYYRFD
                     VIAVLIPENGKPNIRHIPDAFGDE"
     gene            7925..8659
                     /locus_tag="PKW60_00905"
     CDS             7925..8659
                     /locus_tag="PKW60_00905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPJ98018.1"
                     /translation="MEIRTTILKAPEAILFDMGGVLQDASVTWDADTWQRGTPPDLKR
                     PEPFDWFVDMSRDCIERYLALEPPRPAIDVRPVILEWLERSGMDASSEAVDGLFQQML
                     WWEVQPIYGFVRPSLERLHALGFRMGVVSNTLMPGTALRENFASAGILEYFDTVIFSA
                     EAGINKPDPRIFGIALEQMGVRPDAAWFVGDKPQRDVLGAHRAGLTAVLVDSEYADRI
                     HDSPAHMPNVRLRDIAELPEVCAGLS"
     gene            8690..9631
                     /locus_tag="PKW60_00910"
     CDS             8690..9631
                     /locus_tag="PKW60_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPJ98019.1"
                     /translation="MRRLVLCATIGVLAMASCATTARTSPRDARIDVIAHRGASARAP
                     ENTLAAFQLAHELKADWFELDCTLTSDGEILCIHDGSVDRTTNGKGAVAEMTLAELKQ
                     LDAGAWKHPDFAGERLPTLAESLDLARGRIGVYIEIKNSDNDAALEQAILDRTRDLPV
                     LSRAEGDALLALIAESGSRNYELTMKVIDLVRERRMEREIVIQSFSPVCCAVAKLSAP
                     DLRVEFLSGLKPEDHDKWENGARWVFLLGLDGWNANAEGVTAGRLAAMQAAGRSVAVW
                     TVDDVEAMRRFIALGVNAIITNRPDAALGVLHEAHRR"
     gene            complement(9951..10667)
                     /locus_tag="PKW60_00915"
     CDS             complement(9951..10667)
                     /locus_tag="PKW60_00915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPJ98020.1"
                     /translation="MRNLVRQYRTWKHRRKFVSAGKGCRFHGKNLLIDGHVELGDLVK
                     IRDGVIMRTHKGGRILIGDRSGISFYCVFEATTAIVMGKYTGMAEFTVVRDTNHLIYG
                     TAEHWRLTPHLAEPIVIGHCVMIASRCYIGPGVAIGDGAVIAPNSLVTKDVAPMEVWG
                     GNPARKLGHRVKGLVAGAMRRKYGELLETFGIRETPYRDELEAIQEAALSGINRAAEE
                     RDRLVQSLGTQALMAQNGDD"
     gene            10944..11924
                     /locus_tag="PKW60_00920"
     CDS             10944..11924
                     /locus_tag="PKW60_00920"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPJ98021.1"
                     /translation="MNLDRSYTEAVFSVGSPAVVHTNPGECDEKRYPTRVRGWHAGNY
                     VLLDRPMTEDAAELSAKSQRCLLRFVSEGRACGFPCTIFKNSDAAIPYLRVSWPRKLE
                     CVDIRKHQRAQIRVPCKIARVDQPDLAGETTDLSQGGCGIWTDTRLALNTAVQLSFGL
                     PDGSTISGARATVRNVRTIDNGFLLGCMFDEDEPACAVCDFFVTTTIERTRGCKNQKR
                     ALLLEGENDRAEVVRAKLQGKGYHVIRVSCLVDAFFSLRMAFPSCLWVGAEQAEIPVA
                     EICRILRETNGLASLPIYVVGTVSDAIAEDLKRLDVACASSPDVMDRLLT"
     gene            complement(11936..13063)
                     /locus_tag="PKW60_00925"
     CDS             complement(11936..13063)
                     /locus_tag="PKW60_00925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPJ98022.1"
                     /translation="MPHTATLKPKEDRRLLRGHRWAYRNEFAALPDVEDGTVVDVLTD
                     GGRFLGRGFFQAAGGIAVRILTLEDKPVDAALLRARIHRARRYREQRFGDSTVYRWVH
                     AESDGLPGFVADRYDRVVSVQAASVFYAHHEEAIAAAFLEVPGVTGVRFENGGRLTRF
                     GEVISPIELTINDVRFLVDVEQGQKTGMFLDQRENVRLLRNLARDARILDGHCYTGLW
                     GVSAACWGAKQVTCVDTSAAALDLARQNARLNRLEDRCHFQCRDVQGTLRENGPFDVI
                     MLDPPALAKTRGQMRKALGAYLALNRLAMEALAPDGWLITSSCSHFVGADAFLETLTR
                     AARAARRDMWLVEMRGAASDHPVSIAMPETAYLTCAVLRLV"
     gene            complement(13127..14341)
                     /locus_tag="PKW60_00930"
     CDS             complement(13127..14341)
                     /locus_tag="PKW60_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPJ98023.1"
                     /translation="MRRMFILGVAWLAGPALGFAQEAGLWVQVWSYDMPGEQQYLEQL
                     AQVASTEIGEPVRIAFEKWDHAHDAIGQWGKDGQGPDLVVVADIWLAEYAPYFEPLEP
                     LIRPELKDAFYPVLYDKGVYQDALLGLVWATSTKALFYRTDLFEAAGMNPPKTWEEQL
                     GAAVALNDPPRLYGLGLPGKPEYETDDNLFFYFWSAGGEFFDADGKCTLNSSAGVKAL
                     QFYVDLANKYQVTQPELTTWTRKETRRLFEQGRLAMFATGPWGIEQCRKNSPGLKFGV
                     VPLPVDKEQVTQIITDHLVLMRYSPRKELARRFIDIAYRDDQRLAYTTLGLIPEKKTV
                     AQAPHFQDDPHWKVFVDVIPYGRTIPLIQWEKIGIAIREAMYQALSARMTPQDALDAA
                     AARIDEIRAGQQ"
     gene            14994..15299
                     /locus_tag="PKW60_00935"
     CDS             14994..15299
                     /locus_tag="PKW60_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98024.1"
                     /translation="MNDAEFRAVLQHQSQLAEARGAEVSFQEALDDWDQHHALHWREE
                     RQREYLMLQREEIQRHKWIESEKARRDLGAQAVLDWIGTYAESWRAWYEREHDDTRS"
     gene            15560..16531
                     /locus_tag="PKW60_00940"
     CDS             15560..16531
                     /locus_tag="PKW60_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98025.1"
                     /translation="MRCTTPFRTLDYHGRDGKRLAMVALLALCLTAAAAPAGAAALKA
                     DPGVLVFHHTGQSLTAAITRDGHPLPAAEMGEIKLWASGHDYAEMFRYDKLDGALRLT
                     PTGFCEVGSYDLVLGTASGNLNIKVFTPLDEMPETLERLAASMGISLDELRSRMGLVT
                     PLGEVRVDFTLPEVYYEGQALELEFDVIEGASHTWKVNDVVVAQGPDAARLFYVFDKP
                     GLVLVEYTASRAGRTIAAAQDSTMVVPVPAVGWKVPRNVEFVLSAAAGYGKYTWKVNG
                     KPAGEGARLTHKFTEAGPAIIECLCEEPSLGPSGTFLRVTYDVTVTG"
     gene            complement(16593..17243)
                     /locus_tag="PKW60_00945"
     CDS             complement(16593..17243)
                     /locus_tag="PKW60_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013370644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPJ98026.1"
                     /translation="MPARREQTPREELANSLTHGLAAALSIAALTVLVVLSSLQGDPW
                     KIVSFSIYGATLLALYTASTVYHAVPSPRFRPFLRRLDHSCIYLLIAGTYTPFMLVVM
                     RNAWGWTVFGLVWGLAALGILFKLFFMGRYGALSTLWYILMGWIALIAIKPMIAAMPA
                     GVWPWVAGGGLCYTFGVIFYAMGKLRYHHAVWHLFVLSGSLLHFVSLLLFVLPAQG"
BASE COUNT         3270 a         5043 c         5479 g         3541 t
ORIGIN      
        1 gcccccagaa cctccgagcg cgcctttttg agaacgcgct cgcgctggcg ttcgggcaga
       61 tcgctcatat gctcgtccat tcggcgcaga tagcgcccaa tgacgcgctc ttgctggtcc
      121 gtaagtctca tgcctcctcc gcgacgagag aaagtatacc ttaggcggag aaacgggacc
      181 aaatccgggg aaaggcagcc gtgcaaacga ggccgagcct ctgcaacagt tgaaacagcc
      241 cgtgaagctt cgtcccggat ggcttacagc gccgcgactt ccacccggca gtaccaatcc
      301 gggcagggcg cagccactgc cgccatttcc cgcgctgcag acccggtttg agccggagcc
      361 ggggcgctgt tcgcgggcat cagagcggcg atcgatgcgg cggcgcgccg gtcaaccgca
      421 accacatcga agcgcccgcg ggcttggtcg acgaagcctt ccaggggcgt gcagcccgct
      481 tcggttccga tttcgccagc ggtggtttca acctgtcgcg caccggcttc cacggccgcc
      541 agggcattgt ggaccgcgcg gccggaaacg ttgtggcagc tggccgagac gatggcccgc
      601 gactccacga actggacgat atcgcgcacc atttctacgt acgctgcggt tttcatggcg
      661 cccgtgctgt cggagaccgc aatgcgtgtt gcaccggcgc tcacagcaac gcctatgcac
      721 tgccgccgga acggccgttc catggtctcc atgccgccga tcgagaattg gacctcgatt
      781 cccgcagcgc gaacggccgt aacggctctt tctattgcgc ggcaccattc gacgcgcttc
      841 ccgccgctgc gggccgggcc ggacggcagg tagacatgga ccagcggttt cgcggcatgc
      901 ctgaccgcgc cgatcgcggc ggcgatttca gccgggtcgc aggcggccag aaccgccact
      961 tcggacgtgc tgatgcactt tgccacagct ttgacaatgg cgcgctgcga cgaagactgg
     1021 gcggggtatc ccgcctcaat ggttgaaacg cccgcctgtt ccagcgcgcg cgcgatggcc
     1081 gtggcctggt ctaccgaaac gacggggcct gtccacgtga atcgctggga cagcgtggta
     1141 tcgtagattc gaattgctcc gggcatgata catctccttc agacagagtc aatgcggtga
     1201 atttggaaaa gcagccgtca caccgcacca agcgcccgga tctgaggcca ggtagcccca
     1261 catccatacg acaccacctc ccttcagcgg tccccttgat gggaccaaaa aaaaagagcc
     1321 gcgggctttt tcggcccgcg gccttgagtt ccagaaccct tacggggtgt tgattaagga
     1381 attcgaggca taatgggccg accttcctcc acgaggagga gtgagagcag gcggcttata
     1441 tgcttcgaat taaccactat gctaaccctt cttctacttc ttggaaacac tatagcatct
     1501 cttctgcttc ccgtcaaatc gtctttttcc gggcaaatgc gcggtggctc tgcacggagc
     1561 gtagcgcagg cttttctgtt gcgaaactgc aggattctgt ggcacagtga gcgccgggcg
     1621 gcccttcgga ttgggatttc atggaattct gacattgaag gaggagaggc ggcgatgttg
     1681 aaagagttca aagagttcgc gctgcgcgga aacgtggtcg atatggcagt aggtatcgtc
     1741 ataggcggcg cgttcggcac aattgtgaaa agtctggtgg atgacgtgct gatgccccct
     1801 atcggctggg tcgtgggcgg agtggatttt tccaatttct ttttggttat caaggacgga
     1861 agcactccgg gcccttactt ctccctgggg gcggccaaaa ccgccggcgc ggtgactctc
     1921 aattacgggc ttttcgcgaa ttcagtgatc agcttcctca tcgtggcgtt cgcgctcttc
     1981 atggtggtca aggcgatgaa taaattgcac cggaaggaag agcccgccgc cgagcccgcc
     2041 acgaaagaat gcccgtactg cctgtcgtcg attccgctca aggcgagccg ctgcccgcac
     2101 tgcacgtcgg ccattgaagc gatctgaggt tcaagggccg cgccggcaag atgtggcttt
     2161 ggaggggcca attgcgcgat taagaccacc gtatcgcggt atgactctca cgctttgccc
     2221 agcccacgcg ggcgccgtat ggtgcagggc gtccgggcca gaacctcagg gtgaagattg
     2281 ccattagcgg cggcggctgt gctacactga gtggcaaacc ggggagaagg gcgaggggcc
     2341 ttgaagcctg tccaggacga gtgagatacg agatctgcat gtttcgtgtt ccatcctccg
     2401 ccggttcacc cgttgcccgc cggtatcctg ggtccgatac ggcaacccga agaatgaatc
     2461 cgctttgaaa cgccaggcgc agcaggcgaa aagatatttg tagagaagcg cgccgcggag
     2521 gttttttcga gtagaacatg tgcaggcgaa atttactttt tattatgctg ggggtgctgt
     2581 gtgccggagc cgcgggcgcg atggaggcgg gcgcggcgaa ggtagagatc acccccgagc
     2641 tgggcacgcc gcttgctgga agctaccagc gtatggggcg cggcgcgacg gccgtgcatg
     2701 acccgtttta tgtgcgggcc ctttatcttg aagacggcga tacccgggta tttcttgtgt
     2761 cggccgacct ttgcgtgatc agccctgacc tgcgccaggg ggtgctcgaa cgcccgctgc
     2821 acggcgtgcc gcgcgaaaac gttatcctga ccgccaccca cacgatgaat gggccggggg
     2881 ggatggtcta ttctcccgca tttcgggttt tcacggggcg gtacatgccc gagctggtgg
     2941 aatccatggc cgaccgcttc gcagaagcca tggcggaggc ctacggcaac cgaaagcgcg
     3001 ccacgatcgg atttggcacg acggagccgc aggcactgtc tcgcaatgcc gtgaccaaag
     3061 acggtttggt tgacgcccag ctgggcgtgt tgcgtgtgaa tgactccgac ggcaagccgt
     3121 tggccatcgt gggcaatttt ggcgcgcggt gcattggagt caacatcccc gggccggaga
     3181 tgctgacgtt gagcgccgat gtgcccggac gtttctgtga agaactggaa aaactggccg
     3241 aaggatcggt cgcgatgttc ctgcagggcg cctgcggcga tgccgagttc ggcaacccgt
     3301 cgggagcgac cggctgggcg cgcgcggaaa cggcagggcg ggcgctggcc cagcttgcat
     3361 ttgaaactgc gcagacgatc aagagcaatg atcagccgct cgcggtgtct tttgcgaacg
     3421 tggaatttcc tccaaccctg gccgatgcct atttgccttc ggctgccccg ctggccacgc
     3481 tggaaatcgg gtcgctggcg atgacgttca ttcccggcgc tccaggcggc gccatatcaa
     3541 agcagttgcg ttcgttttgc ctcacactcg gctatgaagc ccagtttacc gtgggcgtgg
     3601 cgaacgatta ccggttcttt ttcctgtccg ggcccgaatt ttccgcgccc ggtcctgaga
     3661 gcctgctgtc cttctatggg cccggcatgg gtgactggct gctcacgaat ttctcggggc
     3721 ttctgtcgaa aggcgccgcc ggcgagcccg ccgtccagga agacgtgccc gaagtgcagg
     3781 ccgaaagcgg gatccagcgc atcattctca aaggtacgcc ccatcagctg gggtatgcgc
     3841 ggggagcggc gtttcgtgaa acccttcagg cccagttcga ggagcggtat gtgaaaccgg
     3901 ctgtctccgg cacgctggcc ccgccagaac ccttcgtggc cagtctgccc gtatgcgtga
     3961 ggccgtatct cgcattgtcc aacgtgacgc ttccctggat ggccatgcgg tcgaagacta
     4021 tgctcgctcg cctgccggag ggccagttcg aagagcttgc cggcctggcg gcgggagcgg
     4081 acatgccttt tgacgcggcc tggctgcttc aatgcgcgcc gctgatggag accggggatg
     4141 tgcggggcgg cgcgccgccg gagtcgtttg gcgtcgtgtt cgcggccgtt ggcttgaagg
     4201 ccggagcgga cgacatcctg gtcgggcata atctggacag cccgggacca tttgttcccg
     4261 tggtgtacga tgtgcgtccg gcccaggggc ttcgctattt tctgattggg tctccggcgc
     4321 gttgtggtgg tttcagcggc ataaatgagg aaggcgtggt ggtctgtgta gaacggaacg
     4381 agctgcttgg gtcgcctgcc cagtccgggc cgcccgttga gctggttgca gcgcatatct
     4441 tgagcaaagc ccggaacgcc tgggctgctc tcgatatgat agaaaccttc ccgcacctga
     4501 aaggctacca cattctcctg ggcgaccctg tttcgggcgg caatggccag cctccggcgc
     4561 gagtggtgac gctcgatgcg agcccccgtt tgcgggcgcc tactgagggc acgctgcttg
     4621 gcgccaatcc ggaggcggaa cagttgagcg atgccgcccg cgaccgctat cttcggctgg
     4681 ccgcgctgat cgccgaagag cgcaacctca atccgtctga aatcgaagag attctcctgg
     4741 attctcaaac gagcgtgtcc gaaacgtcag gcgtctttaa ctccaataca cggtatagcg
     4801 ccgtttttga accgaaagcg cggcgcatgc gcgtggcggc gccagggcct gatggcagcc
     4861 ccgggacgta cgttcgcttt gagtttgaat ccggcgccgg ctccaagggc gcggcacggg
     4921 cccgggaaac gggtgggagc gtgcgatgag tgacgcgctg cgcacattcc aggggcctgt
     4981 ccgcggacgc tggtcttcga cgcgccgggg ccgccgccgc cggggaattc ccttcaaacg
     5041 cttctttttt gtggtgctgc ttctgctggc cggcgccggg tattggattt cccgcgacac
     5101 ccatgccgtg cacagcctga taccatcaac gcagaaatac agcgtcgtgc tgcgcgatat
     5161 cctgagaaac cgcgccgagc ttgccggatc ggcgatctgg gacgccctgc ctcctgaaag
     5221 cggttcagga aaaatcaccc aggttctgcg tggggaactc ggtctgcccg aatggatggc
     5281 tcgaaacatc attgtggatg attgctacat cagcgggaac gacctgcgcg atttcaatga
     5341 tgtactgttt gttaccaaga tgacacgtat cggcacgctg atcgaacagc tgcattgggt
     5401 gacatccaga attggccgcg atactgccgg agggctcggg ctgcggcatt ttcctgcggc
     5461 cggtgtgtat tacgccgtgc gcggtcgcat tctgctcatc agcccatcgc gcgatgcgct
     5521 ggtcaacgcg ctgacgctgc aggacgacgg ccgcttgacg gaggcggcgt ttgatcagga
     5581 tttcgaaaag attgctggag aagacattgg cgggacggtg actttcgcgc tcgatgaccc
     5641 gcttggcgag atcctcgaag acatccgttt ctgtttgaga attgatcaga cccaggcgca
     5701 tctcaactgg aacgcccggc tgcgcgccga gcagcacgcg cggcttgcgc ctctgctgtc
     5761 gggggtatcg cccggcacgc ttctgattcc gccgccgggg ctcgtggccg tgtcggggaa
     5821 cctgaacaaa cctcttggcg aagtctggcg cgcgttggac ggttctttca actgcggcgg
     5881 cgggcccggc ctggtatcgg agacgggcga gatcccggag gagaactggc cgaaggaagg
     5941 gccgccttcc ctggcgcagc tcctcgagat gattctgggc ccgctgggcc cgggctggcg
     6001 aatcgcgctt cagggtgtcg acctcaacga atggtttcca gtgcccatat ttgcggggac
     6061 gtttgatctt ccgcagggca cggcttcgga ttttctagcc cagcttccgc ctcctccggg
     6121 cgatgccatg gtatgggaat cctatccacg gtatgacgca gctgctggta tgttgcgcgt
     6181 ccccatgatt gccggcccgt cgctggaacc taccgcagca ccttacggcg agtcgctcct
     6241 ggtctgcagc agcgcctctc ttgccgagga tatactggcg gctgaacctg ccctgcggcc
     6301 tcttgagcag cctggaaact tctacattcg gcttcagccg tcggcctgcg cgcgggccat
     6361 caacgagact ctggaacttc tcgtgcgggg aaacatcctg aaagagcaag ccgtgcggga
     6421 ttttcatgcc gcgttcgacg cctggctcta cggggcggcg cgggtgtcgg aagccgccgg
     6481 tttgcttgct tatgaggacg gggggctgac ggcggatctg gtcgtaattt gtgcgccggc
     6541 gctctagctc tgggatggac aattggcgct acgggcttgc gtggcgcgtg aacggatgag
     6601 gcgtatgccc gaccacatca aagaagagaa tccggacggc gctgccagtc aggaaacgga
     6661 gcctgtttcc actggcggcg aaggcgttat tgtggacgag gcgaccgacg ggattctgcg
     6721 ccttcatctt gacatgttcg aggggccgtt cgaagtcctg ctgtatttaa tcaaagtgca
     6781 ggaaatcgac atcgtagaca ttccgattgt caccattacg gagcaatatc tcggttttct
     6841 ggaattgatg cgcgaggaaa acctggacgt caccggcgag tttctgctga tggcggccac
     6901 cctcatccag atcaagtcgc gcatgctcct tccgccagac cacgacccgg aagatgatga
     6961 agaacaaatc gaggaagaag atcctcgcat tgagctggtt gaaaagctca tcgagtaccg
     7021 gcgctaccgt gacgttgctg cgcggctcga atatctcgaa ggcgaacgtg cccgctggtt
     7081 catgcgcaat tccaagcccg aaatcgagcc ggaagaggac gaagaagagg aactgctcga
     7141 agtgactctg ttcgacctca ttgaggcgtt tcgaggcgtg gtgcggtttt tctccgatga
     7201 tcttttccat tcggttaaga gcgaagaatc ctcggttgac gagaagattg aatatatcga
     7261 gaactcgctc aaggaggatg gcagcctggc ctgggccgac ctgctcggcc agtgccggag
     7321 ccgggtggag ctcgtgtgtt gtttcctggc catcctggaa ctgtgccgga tgggccgtct
     7381 ccgagcgcac cagcatggcc catttgggga gatccgcctc gtagcggccg ggcgggagcc
     7441 cgtggcatga actggcggcc gtggcactgg acacgctccc tggggcagcg cggggaagat
     7501 cttgctgccc gttttttgcg ccgccaggga tactcgattc tggggcgcaa cgtcagtttc
     7561 ggccggtacg agatcgatat cattgcggaa gcaggcgata ccgtggcgtt tgtcgaggtg
     7621 aaaacgcgct gcagcgacga tctggtcgaa cccgaagaaa acgtcggcta catcaagcaa
     7681 cgccatatcc gccgtgcggc ctggcagtac atcgacaagc gcgatgaccc caacctgtac
     7741 taccgttttg acgtcattgc cgtgctgatt cccgaaaacg ggaaacctaa tatccgtcat
     7801 ataccggatg cgttcggcga cgagtgaaca caagcccggc agatgcgagg ttgcgcggtg
     7861 gcgtgttgga aattgtcccg gccaaatgcc tagaatgccc tcacttcatg aatcgggtgc
     7921 tcctgtggaa atcagaacaa caatcctgaa agctccagag gctattctat tcgacatggg
     7981 tggcgtattg caggatgcca gcgtcacgtg ggatgcggat acctggcagc gcggaacgcc
     8041 gccggacctc aaacgcccgg aaccgttcga ctggtttgtc gacatgtcga gggattgtat
     8101 cgaacgttat ctggctctgg aaccgccccg tcccgccata gatgttcgtc ccgttattct
     8161 ggaatggctc gaacggagcg gcatggacgc ttcttccgag gcggtagacg gcttgtttca
     8221 acagatgttg tggtgggagg ttcagccgat ttacggattt gtgcggccct ctcttgaacg
     8281 gctgcacgcg ctgggtttcc gaatgggcgt ggtatccaac acgctcatgc ccggcaccgc
     8341 cctgcgcgaa aatttcgcat ctgcggggat actcgagtat tttgacacgg tgattttttc
     8401 ggccgaggcc ggcatcaata agcccgaccc gcgcattttc ggcatagccc tggagcagat
     8461 gggcgtccgc ccggacgccg cctggtttgt gggagacaag ccgcagcgcg acgtgttggg
     8521 cgcccatcgt gcgggtctga cggccgtgct ggtggacagt gaatatgcgg accgcatcca
     8581 tgacagtccg gcccacatgc cgaacgtgcg tctgcgcgat atcgcagagt tgccggaggt
     8641 ctgtgcaggt ctgtcataaa ggtctctgga gcaggaagga gtcaaatgca tgagacgact
     8701 tgttttgtgt gcaacgattg gagtgcttgc catggctagt tgtgccacca ccgccaggac
     8761 cagcccgcgg gacgcgcgta ttgatgtcat cgcccaccgt ggcgcaagcg ccagagcccc
     8821 tgaaaatacc ctcgcggcgt ttcagcttgc tcatgagttg aaggccgact ggtttgagct
     8881 ggactgcacc ctgacgagcg acggcgagat cctgtgcatc catgacggca gcgttgatcg
     8941 aaccacgaat ggcaagggcg cggtagctga aatgaccctc gccgagctga agcaattgga
     9001 tgccggcgcc tggaaacatc ccgattttgc cggcgaacgg cttcccactc tcgcagagtc
     9061 tcttgacctc gcccggggcc gtatcggcgt gtatatcgag ataaaaaaca gcgacaacga
     9121 cgctgcgctt gagcaggcca tccttgaccg cacgcgcgac ctgcccgtcc tgtcccgcgc
     9181 cgaaggagat gcactgctcg cgctcattgc cgagagcggc tcgcgcaatt atgaactcac
     9241 catgaaagtc atcgatctgg tgcgggaacg ccgcatggag agggagattg tcatccagtc
     9301 cttttcgccg gtctgttgcg cggtggccaa gctgtcggcg cccgatctgc gggtggagtt
     9361 tctcagcgga ttaaagccgg aggatcacga caaatgggag aacggcgcgc gctgggtgtt
     9421 cctcctcggg ctggatggct ggaacgcgaa cgctgaaggc gtcaccgccg gccgtcttgc
     9481 cgccatgcag gccgcaggcc gatcggtggc cgtgtggacc gtcgatgacg ttgaggccat
     9541 gcgccggttc attgcactcg gggttaacgc catcattacc aaccggcccg acgctgcgct
     9601 gggtgttctt cacgaagcgc accggcgctg acgcacttcc ggcggcggtc tgggggccag
     9661 ggcagaaaca ctggttgagg gtggcgcttt cgcactgcgc gggcaacttt gcagacagcg
     9721 ccgccggcat tcggtatgga gatcactagc cggaacctgt caacgaagcg gactgagcca
     9781 acaggcaatg tgtctccagc cttccccgag cccatggccg gattgcgggt agtgcggaca
     9841 atggggagcc cgtggggccg gaccccattt attgcgatgg gacaccgttg cgcataccaa
     9901 acctctgcgc gggggacagc cagagtctgg cgcgccagct ccctggagct tcagtcatcg
     9961 ccgttctgag ccatcagcgc ttgggtgccc agcgattgga ccaggcgatc acgctcttcc
    10021 gccgcgcggt taatgccgct gagcgcggcc tcctggatgg cttccagttc gtcccggtag
    10081 ggcgtctctc tgatcccgaa agtttccagg agttccccgt actttcgccg catcgccccg
    10141 gccacgagcc ctttgacgcg atggcccaat tttcgtgcgg gatttcctcc ccagacctcc
    10201 atgggcgcga cgtcttttgt cacgaggctg ttcggtgcga tcacggcccc gtcgccgatg
    10261 gcaacccccg ggccaatgta acaccggctt gctatcatca cgcagtgccc aatcacgatg
    10321 ggttccgcga gatgcggtgt caggcgccaa tgttccgccg tgccgtagat taagtggttc
    10381 gtatcccgca ccacggtgaa ttcggccata ccggtatatt tgcccatcac aatggcggtg
    10441 gttgcctcaa agacgcagta gaagctgatc ccggatcggt cgccgatgag gattcgccct
    10501 cctttgtgcg tgcgcatgat cacgccgtcg cgaattttga ccagatcccc cagttctacg
    10561 tgcccgtcga ttagcagatt ctttccgtgg aaccggcatc cttttcctgc cgacacgaat
    10621 ttacgccgat gtttccacgt gcggtattgg cgcacaagat ttcgcatgag tgactccttc
    10681 tttggcgccc agtatatcca tgtctttgtg agcacacaac gactccggca atctgatgct
    10741 ctgggtatcc cgcctgaatg agactttgcg ttgctctgtg ccgagaaact gttatgagca
    10801 gtgctctata tccaccgaac gacttgtctg ggaccgactc ttttggtatg ctaatgtaga
    10861 tataattgtg ggggtaattc gccaatatat gggggatggc acagcggggc tcaaggttgc
    10921 accatacagt gaaaacagga aatatgaacc tcgatcgcag ttataccgaa gccgtgtttt
    10981 cggtaggttc tcccgcggtc gttcacacga atccggggga atgcgacgaa aaacggtatc
    11041 cgacccgggt gcggggctgg cacgctggaa attacgttct gcttgaccgc ccgatgacgg
    11101 aagacgctgc ggagctgtcc gccaaatcgc agcgttgtct gcttcgcttc gtgtcggagg
    11161 ggcgcgcctg cggctttcca tgtacgatct tcaaaaacag tgacgccgcc attccctatt
    11221 tacgcgtgtc gtggccgcgc aagcttgagt gcgtggatat ccggaagcac cagcgcgcgc
    11281 aaattcgtgt accgtgcaag atcgcgcgcg tggaccagcc ggacctggcc ggagaaacga
    11341 ccgatttgag ccagggcggg tgtggcatct ggacggacac acgcctggct ttgaacaccg
    11401 ccgttcaact ttcgttcggc ctgcctgacg gcagcacgat ctccggcgcg cgagccacag
    11461 tgcgaaatgt gcgcaccatt gataatggat ttcttttggg ctgcatgttc gatgaagacg
    11521 agccggcatg cgccgtttgc gacttttttg tcaccaccac gattgaacgc acgcgcggct
    11581 gcaagaatca gaaacgcgcg ttgctgctcg aaggcgagaa cgaccgggcc gaggttgtgc
    11641 gcgcaaagct ccagggcaaa ggatatcacg taatccgggt gtcctgtctg gtagacgcct
    11701 ttttcagcct gcgcatggcg ttccccagct gcctgtgggt gggggccgag caggcagaga
    11761 tcccggtcgc cgagatctgc cgtattctgc gcgagaccaa cggcctggca tctctgccca
    11821 tctacgtcgt cggcacggtc agtgatgcca ttgctgaaga cctcaagcga ctggacgtcg
    11881 cctgcgcgtc atctccggat gtcatggacc ggcttctcac ctgagctcac ggggctcaga
    11941 ccaggcgcag taccgcgcag gtcaggtagg ctgtttccgg catggctatc gacaccggat
    12001 gatccgaggc cgctccccgc atttcgacga gccacatgtc acgccgcgcg gctcgtgctg
    12061 cccgtgtgag tgtctccaga aatgcatctg cgcccacaaa atgcgagcag gatgacgtga
    12121 tcagccatcc gtccggggcg agtgcttcca tcgcgaggcg gttcagggcc aggtaggcgc
    12181 ccagcgcttt gcgcatttgc ccgcgcgttt tggcaagggc gggcgggtcc agcatgatga
    12241 cgtcgaaggg tccgttttcg cgcaacgtac cctggacgtc cctgcactgg aagtggcagc
    12301 ggtcttccag gcggttcaac cgggcgttct gccgggccag gtcgagcgcc gcggccgatg
    12361 tatccacgca ggtgacctgt tttgcacccc agcaggcagc cgagacgccc cagagcccgg
    12421 tgtagcagtg gccatcgagt atccgggcgt cccgcgccag attgcggagc aggcggacgt
    12481 tttcacgctg atcgagaaac atcccggtct tctgcccttg ttcgacatcg accagaaaac
    12541 gtacgtcgtt gatagtcagc tcgatcgggg aaataacctc tccgaagcgg gtcaggcggc
    12601 cgccgttttc gaagcgaacg cccgttacgc ccgggacttc caggaatgcc gccgcgatgg
    12661 cctcttcgtg gtgcgcataa aacacgctgg ctgcctgcac ggagaccacg cgatcatagc
    12721 ggtcggccac aaaaccgggc aggccgtcgc tttccgcgtg aacccagcgg tagactgttg
    12781 aatcgccgaa tcgttgttcg cggtagcgtc tggcgcgatg aatgcgtgcg cgcagcagcg
    12841 ccgcgtcgac gggtttgtcc tccaatgtca atatgcgcac ggcgataccg ccggccgcct
    12901 ggaaaaaacc gcgtccaaga aagcggccgc cgtccgtgag cacgtcgacc acagtgccat
    12961 cctccacgtc tggcagcgcg gcgaactcat tccggtaggc ccagcggtgt ccgcggagga
    13021 gccgtcgatc ttctttcggt ttcagcgtgg ccgtgtgcgg catggcgtgt ctttcatcgc
    13081 agcggacatc gcgattgtgc cgccgcgcag ttttgtgttg cgagttctat tgctgcccgg
    13141 cgcgtatctc gtcgatgcgg gctgccgcgg cgtcgagggc gtcctggggc gtcatgcggg
    13201 cgctcagggc ctggtacatg gcctcgcgga tggcaatccc gattttctcc cattggatga
    13261 gcgggatggt gcggccatac gggatcacat caacgaatac cttccaatgc gggtcatcct
    13321 ggaagtgggg cgcctgggcg accgtttttt tctcaggaat caagccgagc gtggtgtacg
    13381 ccagccgctg gtcgtcgcgg taggcaatat caatgaaccg ccgggccagc tcttttcggg
    13441 gagaataccg catcaacacg aggtggtcgg tgatgatctg ggtcacttgc tccttgtcga
    13501 caggcaatgg taccacgcca aatttgagcc cgggagaatt ttttcggcat tgctcaattc
    13561 cccagggtcc ggtcgcaaac attgcaaggc ggccctgttc aaacagccgc cgggtctctt
    13621 tgcgtgtcca ggtcgtcaac tccggctggg tgacctgata tttgttcgcc agatcgacgt
    13681 agaattgcag ggctttgacg ccggcactgc tgttcagtgt gcatttgccg tccgcatcaa
    13741 agaattcgcc ccccgccgac cagaaataga agaacagatt gtcgtccgtc tcgtattcgg
    13801 gcttgccggg cagtcccagg ccgtacagac gcggcgggtc gttcagcgcg accgcggcgc
    13861 ctaattgctc ttcccaggtc ttcggcggat tcatgcccgc ggcctcaaac aggtccgtgc
    13921 ggtagaagag tgctttggtc gacgttgccc acacaagtcc cagcagcgca tcttgataga
    13981 cgcctttgtc gtacagaacc ggatagaagg cgtctttcag ttcggggcgg atcaatggct
    14041 ccagcggttc gaaatacggg gcgtattcgg ccagccagat gtccgccacc acgacgaggt
    14101 cgggcccctg gccgtctttc ccccattggc cgatggcgtc gtgggcgtga tcccatttct
    14161 caaacgcgat gcgaaccggc tccccgattt ccgttgaagc cacctgagca agctgttcca
    14221 gatactgctg ctcgcccggc atgtcgtacg accacacctg cacccaaagg ccggcctcct
    14281 gagcaaaacc cagagccggt cccgcgagcc aggcgactcc caggatgaac attcttctca
    14341 ttggattctc tctttctcga tagcgcggcc agcgcgtccc cagttccttg tcagtcatct
    14401 tagcgcgcct ggctcagata gagcaaagcg gcgttcgggt gagacgcccg tcatgattcg
    14461 gctggatcct caacgctgtg cgcagagcgc ctgttttgtc atcaggcggg agatgatggc
    14521 cgagccctgg cagcggccgc agatggcagc tcccttttgt gcctaagctg cgatttggta
    14581 atcacttgcg cgaacaaggc gactgtccgg gcgctaatca ctctggcgga gtcttcgggt
    14641 catcagtgcg aaaaagctat agaagccggc tgtaatgcgg tatatgcagt gcttccggtg
    14701 ttttaccggt gaatatatcg aagaaaatag cgtcggtatt ttttgagtct tatggagtat
    14761 gcgcgttgaa aaatcgagaa agaccggtgg gtcagcagga attttggatg tttttttgga
    14821 ctcggcgtgt cggcgtgtta taatttcgtg aatgctctgt actgttttgc aaggtttgaa
    14881 gagaggtatt ctggatggct ggttcaggat ttgcgcaacg cgttcagggg attagcgctt
    14941 cggcgggggc ggcgccaaaa ggcggttttg ttcgaccagc agggggatcg ggattgaacg
    15001 acgcagaatt cagggcggtg ctccagcatc agagccaact ggcagaggcc cgaggcgcag
    15061 aggtgagttt ccaggaagcg cttgacgact gggatcagca tcatgcgttg cactggcggg
    15121 aggaacgcca gcgcgaatac ctcatgctgc agcgcgagga aatccagcgg cacaagtgga
    15181 tcgaatccga aaaagcgcgg cgggaccttg gcgctcaagc ggttctggat tggatcggca
    15241 cctatgcgga atcctggcgt gcctggtacg agcgggagca cgacgacacc cggagttagc
    15301 cgcttgcccg cgatgccgtt ccggggggga cgtgagggga gtctgtattc caacagccag
    15361 cctgcgcagc tgcggctttg aactgcactt ctctttttct atcttccgcc ttgcccagcc
    15421 agagaaacca tgagagatgg ccggggatta tactgaacac cagcgcaaga cttgctatgc
    15481 ttactgcggg cgccagccgt gggccgcgaa ctgagcagcg gggcgagaaa cgaatcttaa
    15541 ccggacggag atgtcgagca tgaggtgcac aacgccattc aggacgctgg attaccacgg
    15601 gcgtgatgga aagagactgg ccatggttgc tctgctcgcc ttatgcctta ccgctgcagc
    15661 ggcgccggct ggtgcggccg ccttgaaagc cgatcccggc gtcctggtct ttcaccatac
    15721 cggccaatcg cttacggcgg cgatcacgcg cgacggccac cctctgcccg cagcagagat
    15781 gggagagatc aagctctggg cgagtggcca cgattacgcc gagatgtttc gttacgacaa
    15841 attagatggc gcgctgaggc tcactcccac ggggttttgc gaagtcggga gttatgacct
    15901 ggtgctgggc acggcgtcgg gtaacctgaa catcaaagtg tttaccccgc tggacgaaat
    15961 gcccgagacg ctggagagac tggcggcctc catgggcatc agcctggatg agttacgaag
    16021 ccgaatgggc ctggtgactc cgctcggcga agtgagggtc gatttcacgc ttccggaagt
    16081 ctattacgaa gggcaggcgc tggagctgga atttgatgtc atcgaagggg catcccacac
    16141 ctggaaagtc aatgatgtgg ttgtcgcaca gggccctgac gccgcccgat tgttttacgt
    16201 gttcgataaa ccgggactgg ttcttgtgga atacaccgcc tcgcgggcag gccggacgat
    16261 tgcggccgcg caggactcga ccatggtggt tccggtgccg gcggtcggct ggaaagtgcc
    16321 ccggaacgtt gagttcgtct tgagcgcagc tgcgggatat gggaaatata cgtggaaggt
    16381 caatggaaaa cccgcgggag agggggctcg attgacgcac aaatttacgg aagcaggacc
    16441 cgcgatcatc gaatgccttt gtgaagagcc gtccctgggt ccgtcaggga cctttctgcg
    16501 cgtcacttac gacgttaccg ttaccggtta gcgcggcccc tgcgcgggct tgcgggcgcg
    16561 ctgcgctcag gaacaccggg catgccatcg ttctatcctt gtgccggcag cacaaaaagc
    16621 agcagcgaga cgaagtgcaa caagctgccg ctcagaacaa acagatgcca gacggcgtgg
    16681 tggtagcgga gctttcccat ggcgtaaaag atgacgccga acgtgtagca cagtcctccg
    16741 ccggccaccc agggccagac gcccgccggc atggccgcaa tcatgggttt gatggcgatg
    16801 agcgcaatcc atcccatcaa aatgtaccac agcgtcgaca aggcgccgta gcggcccata
    16861 aaaaacagtt tgaacagaat ccccagcgca gccaggcccc aaaccagccc gaacaccgtc
    16921 cagccccacg cgttgcgcat gacgaccagc atgaacggcg tgtaggtgcc cgcaataagc
    16981 aggtagatgc acgaatgatc cagacggcgt agaaatggcc gaaagcgcgg agacggcacg
    17041 gcgtggtata cggtggaggc cgtgtacagg gcgaggagcg tcgcgccgta aatgctgaaa
    17101 ctcacgattt tccatggatc gccctggaga ctggacaaca ccacgagcac cgtgagtgcg
    17161 gcgatgctca acgccgcggc gaggccgtgg gtcaggctgt tcgccagttc ttctctgggc
    17221 gtttgttctc ttcgtgcagg catgggacgt ccgggccttt ccccgtgcat ggccgattac
    17281 ggcgtgcgcc ttgattattc cggccggcag ctactcgacg gctcgggggc cgt
//
LOCUS       DAOPBQ010000012        17129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_3405_length_17129_cov_3.693745, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000012 DAOPBQ010000000
VERSION     DAOPBQ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 17129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17129
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3405_length_17129_cov_3.693745"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /gene="rsgA"
                     /locus_tag="PKW60_00950"
     CDS             complement(<1..922)
                     /gene="rsgA"
                     /locus_tag="PKW60_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPJ98027.1"
                     /translation="MKDRRRKKPRVRKRDWSEADLDAETRQPRRFRGLDVGLGAEAPD
                     IDERFEDIQPNGVVISPYGVLAFVEIDGQEQLCRVAERLCTGKTSILAPGDLVLVDAL
                     DDDLCVSAVQRRRSKLSRPASKGQREQVIAVNVDHIVCVVAVAQPRFKAGVLDRLLIA
                     ADIGGIHPIVVVNKMDLTGETPREFEVYRDLELPLIFASCETGEGIDELRRLLENSVS
                     VLAGQSGVGKTSLINCLHPDFELETQAVSAFNEKGRHTTTTSRLYHLGGGIDIIDTPG
                     IRQLGVWALAKEEAALYFPDIAVLGERCRFR"
     gene            complement(919..1686)
                     /locus_tag="PKW60_00955"
     CDS             complement(919..1686)
                     /locus_tag="PKW60_00955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPJ98028.1"
                     /translation="MSDPAAGAKIDYDQIARRYDSFRGGRGPYQEFLVELAQAHDARR
                     ILEVGAGTGNNMFAFRAAYPAASFFACDLSRGMILRAREKGAPGAWLRADARHLPFAA
                     QSFDLCFGCYMLHYIDDLNALASECARVLRTGCAAFITASHEFIERHPMNRYFPSFAA
                     IDKARFPDINVVKDGLARAGFLEPAVERLAVPQPPIDANYLTRVENRFISTFDLLPEE
                     EFNAGLTRLRTDIQTQGSLDIENVWESVVVWARKGAE"
     gene            complement(1683..>4440)
                     /gene="glyS"
                     /locus_tag="PKW60_00960"
     CDS             complement(1683..>4440)
                     /gene="glyS"
                     /locus_tag="PKW60_00960"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPJ98029.1"
                     /translation="LEALGLDLTRHDYRFVEDNWQSPSLGAWGLGWEAWLDGMEILQF
                     TYFQECGGLKLDVRACELTYGIERIAMYLQGVESVYDLKWAPSGITYGDIYHAAEVEW
                     CKYNFEAADTGKLLQVFDLWEAEAARLLELGLIMPAYDHCLRLSHLFNLLDARGALSV
                     TERGRFLLRCRAVAERCAKGFFEQRQEKGFPLCRLPRPWREAEASPVQADPGAFQERD
                     TLLLEIGVEELPHSDVKAVRAQLPALMTKLFQEARLQHGPVTVWVTPRRIVARLDDCA
                     SQQQDLMEEARGPARKAALDADGQWTVPARKFAESKGARVEDIYFQEQGKAEYCFIKV
                     ERKGQHITELLSGIVTKLLAGLQFVKSMGWEDSTVTFSRPVRWLLALHGANPVPVSWK
                     FVDGGEFAAERFLHSGTVSYGHRRLAAGPVSIPSAGDYLETMKRHFVLPVLDERMARL
                     RSEAGAIASAQGLHLDEDSDLLEEIIDITEWPEPILCSIPDDALALPEEIVITPMKVH
                     QRYIPLRDAQGGLSRHFLAVANGKHDEEGRATIRRGNERVLNARLRDARYFWDTDTRK
                     PLREFAAGLSRVLFHQKLGTVADKISRLHRLYPMLKNVLPAVDDARVAQVLDLMKADL
                     TTQMVFEFDSLEGVVGMLYARKEGLPEDIALALFEHRLPRRAGDRLPKSPLGIVAAVM
                     DRTDTLAGYFGLGIRVKGTSDPFALRRNTLALLSIILEEGLDIDLEEAFRSALSVYDG
                     ILAEPEAAVDVFLQFAADRLAVIHREKGIPYDQVAAAIASHGRRPVQVRRCLEAFSRL
                     DEQQVQLLAEQTKRIQRIALEPAPAIDSALLEVNEKALYTLTGEPSARLRDLVASGAF
                     ETAFSEVLSWMPVIEAYFEDVFVNHDDKSVRQNRHGLLKTIFSAISVAADFTQIEKKE
                     TPAE"
     assembly_gap    4441..4450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4451..4727)
                     /locus_tag="PKW60_00965"
     CDS             complement(<4451..4727)
                     /locus_tag="PKW60_00965"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPJ98030.1"
                     /translation="MITVQEVLQTLNSFWASRGCILWQPYHTEVGAGTSNPATFLRVL
                     GPEPWWVAYPEPSIRPTDGRYGENPNRLQHYYQYQVILKPSPPNVQEL"
     gene            complement(4943..5731)
                     /locus_tag="PKW60_00970"
     CDS             complement(4943..5731)
                     /locus_tag="PKW60_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HPJ98031.1"
                     /translation="MRRPVAMITGASRGIGRGIAIELAKNGFDLIGTATRLTPEDAEK
                     GLFEVKARAEELGARFVPLAGDIGEMDDRNRLVDEAFAAFGRIDLLVNNAGVGLLKRL
                     DILETTEESFDRLVRINLKGPFFLTQMIANRMIDRVTAGDGDPPSIIFITSISSNTAS
                     PNRAEYCISKAGLSMAAQTFAVRLAEFGINVYDIRPGIIATDMTAPVSEKYDKLISEG
                     LLLQKRWGTPEDVGKAVASIAGGHFPYSTGAVIEVGGGFSVLRL"
     gene            5953..6837
                     /locus_tag="PKW60_00975"
     CDS             5953..6837
                     /locus_tag="PKW60_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPJ98032.1"
                     /translation="MQATRRDFLLQTGFGIAATGFGLSRAMAEDGKPALRLSACDWSL
                     GAWGPEGLALAAAVGLDGLEISAATDNGSPGDVLAVADAERRAAYKEQSKEHGVVVSS
                     VAMGLLNGFPMVSDPRAMAWTEQTIDGTKDLGARVMLMAFFGKGDLRDKSGALKTQDV
                     DTLIGQLKELAPKAEKAGVILGLENTLSAEQNLDILGRVQSNAVRVYYDVGNSTANGY
                     DVPAEISLLRDRICQIHFKDNKGHLGEGEVDMSAAGRAMRDIGYAGWVVLETKVLGER
                     DADFRRNAAFTRALLGNA"
     gene            6948..7697
                     /locus_tag="PKW60_00980"
     CDS             6948..7697
                     /locus_tag="PKW60_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPJ98033.1"
                     /translation="MIRTERLRKVYFPPRSKPVEGVKNATFSVEPGEIFGLLGPNGAG
                     KTTLLRMLGTIIVPTSGYCWIAGVRTDQNPDAVRQHIGFLSGNTKLYGRLTAREELRY
                     FGDLHGLPKDRIRQRTEELAEILGMGEFIDRRCDTLSTGQSQKVSIARVLLHDPEVLI
                     LDEPTTGLDIMTCQTILSFIRNAKHYGRSILFSTHYMTEAEILCDRLAFIYSGEILAT
                     GTKESLYEKTDTTNLTDAFVRLVNAANTVPS"
     gene            7694..9736
                     /locus_tag="PKW60_00985"
     CDS             7694..9736
                     /locus_tag="PKW60_00985"
                     /inference="COORDINATES: protein motif:HMM:NF041647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HPJ98034.1"
                     /translation="MKMRTVRAIFFKELMVMLRDKRTLVAMIGIPVVLYPVLLVVTSQ
                     VALMHISHVEESTSRIAIKAGQDPTVAHWIDALPRMKLVSMEKPEAALLDGEVDAVVV
                     VKQRAEEVLERGGSLLIVIQYDSTVPQSREAAHRLSEGLRTTQTRLLEDRLAKAGITL
                     DFVTPLKLQEMNVAPAARIVGTLLAAMLPMIMVLMVGIGAFYPAVDLTAGEKERGTFE
                     TLLSTPATKFEIVCGKFLTVFCISMLTGLLNLASMAMTFIFHLSDLLTRLQVGPEKAP
                     VQFPLGTALLMTLLLIPLALFVSALMMSVAVLARTFKEAQNYVTPFFLLITIPAGLAG
                     MPGLELNSVLQFVPIVNIGLLFRDLLTDRAKLEAVFSVLLSTSAFALLALVCATWLFQ
                     REDVILSEEKGVPLTLRRSRIAPRGEPTVGMAMTLYTLILLLIFYVGVYAQSKHMLGG
                     LLITEWLVIALPTVALLWYLRVDLLSALHLRAFRIDGALATAVIALSWLVLAFQLGLW
                     HSNVLPMPEDMAKQYLQLIHLEDTSLVVLLAVIAVSPAICEEILFRGAILSGLEKRLP
                     AWLLIPVVGLLFGAFHLSIYRVLLTGLTGMVLTYVVWRTRSLWNGILLHCLFNASAVL
                     MASERLPEKLLTGLRNLSAAQAGLPWWLIGLAASAMSAGIGYLHLRFPDPNRGPSE"
     gene            complement(9728..10984)
                     /locus_tag="PKW60_00990"
     CDS             complement(9728..10984)
                     /locus_tag="PKW60_00990"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPJ98035.1"
                     /translation="MKPSLFLSRNQVLAVQREFGTPVYVYDEATLEHQARQALEFPCP
                     FGLTARFAMKALPNLSILRLLHGLGLHFDASSGYEAERAMRAGIPPEHIEVTAQELPH
                     NLTQLVEAGVLFNACSLAQLRAFGERFPGASLGVRINPGLGSGHNNRTNVGGPSSSFG
                     IWHEYIPKVLDAASALNLHITRVHTHIGSGGEPSVWTQCAKLSLEAAARFPEVATLNL
                     GGGFKVARMPGESAANLQEIGGAVSKILREYAESTGKELRLEIEPGTFIVANAGVLIT
                     TVMDVVDTGNEGYRFIKADAGMTDILRPALYGAQHPIALVTASGEERPGGEYLIAGHC
                     CESGDLLTPEPGNPEGLLPRALPEPRIGDALAIGGAGAYCSAMCSKNYNSFPESAEVL
                     LRKNGEVHLIRKRQTLDQMLANELLF"
     assembly_gap    11240..11249
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11250..12542
                     /locus_tag="PKW60_00995"
     CDS             <11250..12542
                     /locus_tag="PKW60_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98036.1"
                     /translation="KRYPEAMIALDELNASFPDEKHVMLARAMCLAELQRPLEAMQIC
                     ERAVAMHQDPKAEAYLARLHREHGPLQHGPTDLASLLGQGRKAGAPGAAAGLGAMPAI
                     PDVPDLTIYNPPQQSSSKPFYIILGAVAAVLVVLLIGLPLLLSLKKKGGEAPQSGAMT
                     TVEQATPTETGALPDPAGTEVQPVDAAAAAPPVQWYTNYEQGTNVATAHGHPICLFFS
                     EDSEASRAVESSVFEDPGIRQSLSQFINIRVSEAEKEPAAAQYGIEQFPAIVVVGLGN
                     NIVYRAQGGKIDPAELRSALKGMQMAQGAFKMWPFTGGIPVFAVVVMGLLSLLLTPWP
                     LYLTLLFTGKLPHGQFVKDVFSVALVAIGANAVAGFVPCMGIFVLIWILHAVYDLGLV
                     DFLIYFALSTVMWVILALITFATMGSTILSALSEIPAM"
     gene            12969..14330
                     /gene="radA"
                     /locus_tag="PKW60_01000"
     CDS             12969..14330
                     /gene="radA"
                     /locus_tag="PKW60_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPJ98037.1"
                     /translation="MAKTRSTFVCQSCGATYMRWVGRCTDCGQWNTVLEEVVVESGKH
                     PRTPTGDQGAPLPITDQQHEPPPRLSTGILECDRVLGGGIVPGSLTLIGGDPGIGKST
                     MMLQISHGLAQDGGPVLYISGEESFSQSQLRARRLGTLSDELFMLTETELASLRKYIQ
                     GGTFSLVVVDSIQSVYSADLQGVPGSISQVRDCASEFLRLAKSSGTPIFLIGHVTKDG
                     TIAGPRMLEHLVDTVLYFEGEGKQALRILRAVKNRFGSTNEIGVFEMTETGLQEVPNP
                     SALFLNERPEGVSGSVVLPSVEGTRPLLVEVQALVCDSNLGSPRRTVTGVNPNRVSLI
                     LAVLEKRAGLHFADRDVFVNVAGGVRLDEPAADLAIALALVSSFMEAPVAPHLTAFGE
                     IGLAGEIRGVDMARRRVAEAAKFGFTRCILPKNNATDVENADEQLWPVGRIGPAIELA
                     LER"
     gene            14394..15479
                     /gene="disA"
                     /locus_tag="PKW60_01005"
     CDS             14394..15479
                     /gene="disA"
                     /locus_tag="PKW60_01005"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HPJ98038.1"
                     /translation="MVRPKRKTKSIAFHEAIRMISPGTKIREAISMMLQSRLGALVCI
                     GDPKRLADLSGGGVELNAACTPQLLYELSKMDGAIILNEDGSTITYANRFLTPSNSIV
                     SQETGTRHIAAERMAKQAKCAVVAVSERRSSVTLYVQNTRHVLDSLATLLNKSSQALQ
                     TLEKYISSLNQSMVDLSTREFEDMVTIFDVCKAIQRCEMSRRIAAEIEPYILELGTEG
                     RLIELQLRELSIPIEEARLITKDYYKQKPSANPEAVRQRVEELPQDELISLEAISQAL
                     GYGANLRGVDTYLSSRGYRVLAQTRRLTPQIIENLVNRFGTLQQIMRAPKDELVAVEG
                     VGEVLAERVRLSLNNLRSQLALDRGRT"
     gene            15481..16290
                     /locus_tag="PKW60_01010"
     CDS             15481..16290
                     /locus_tag="PKW60_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPJ98039.1"
                     /translation="MRYIQDCEVVTHDQAAPSHWRMRLRSEDIARAAKPGQFCMVEVA
                     ETLSPFLRRPMCFSEIHDDGFTLLYKIEGEGTRLLSQRVPGQTCSIQGPLGNGFPIVP
                     EIPRHIIVSGGIGVAPFPALAQALTDACGRPPEVVLAARNRELLLCAEQFESFGCTVY
                     LATDDGSAGKKAYAAQMLERLAPDPGTRIYACGPMVMMSATAAVAAQAGASCLVSLEA
                     QMACGDGTCLSCVVEAKEEREGEKMVRVCVDGPVFDAALIDWEAHSTAYDL"
     gene            16328..>17129
                     /locus_tag="PKW60_01015"
     CDS             16328..>17129
                     /locus_tag="PKW60_01015"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPJ98040.1"
                     /translation="MKNPVTVASGTFGYGEEYGRYFDVARLGAVTTKSISLKPRAGNK
                     PPRLAETPAGLLNAIGLQNVGVERFLSQKVPYLRKCNATIIANIYGHSPEEYAALARR
                     LEAEGAADAIEANLSCPNVHDARSARGAVLVSQDCQAITEYTRAIREATRLPLYIKLT
                     PNVMNIQEPALAAEAAGADGVCLINTLLGMAINPETRKPRLANIVGGLSGPAIRPVAV
                     KMVWDAARVLRIPIIGTGGITDAASAIEFLLAGATAISVGSMSFRQPDA"
BASE COUNT         3305 a         5066 c         5384 g         3354 t
ORIGIN      
        1 cgcggaacct gcaccgttct cccagcacgg cgatatcggg gaaatagagc gccgcctctt
       61 cttttgcgag ggcccagacg cccaattgac gtatgcccgg ggtgtctatg atgtcgatac
      121 cgccacccag atgatacagg cgtgacgttg tggtggtgtg gcgtcccttt tcgttgaatg
      181 cgctgacagc ctgggtttcg agctcaaaat ccgggtgcag gcagttaatg agcgatgtct
      241 tgcctacgcc gctctgtccg gcgagcacgg atacggaatt ctcgaggagc ctccgtaatt
      301 cgtcaatccc ctcccccgtt tcgcaactgg caaagatgag gggcagctcc aagtcgcggt
      361 agacctcgaa ctctcgcggc gtttcgccgg tcagatccat cttgttaacg accacgatgg
      421 gatggatccc cccgatgtcg gccgcaatca gaaggcgatc cagtaccccg gccttgaatc
      481 gcggctgcgc gacggcaacc acgcagacaa tatggtccac attcacggcg atcacctgtt
      541 cgcgctggcc tttgcttgcg ggccggctca gcttgctccg ccgccgttgg acggcagaaa
      601 cgcacaggtc atcgtcgagg gcatccacga ggaccagatc gccaggcgcc aggatggatg
      661 tttttcctgt gcacaggcgc tccgcgaccc ggcagagctg ttcctgcccg tcaatctcca
      721 caaacgccag gacgccgtac ggcgaaatga ctaccccatt gggctgaatg tcttcaaagc
      781 gctcgtcgat gtccggggcc tcggctccca ggccgacgtc caatccgcga aagcggcgcg
      841 gctggcgagt ctccgcatcg aggtccgctt cggaccagtc gcgcttgcgc acgcggggtt
      901 tctttcgccg gcgatctttc attcagcgcc cttccgcgcc cacaccacga cgctttccca
      961 cacgttttcg atgtcaagac tgccctgggt ctggatgtcc gttcgaaggc gggtgagacc
     1021 cgcattaaat tcttcttcgg gcagcaggtc aaacgtcgag atgaatcgat tctcgacgcg
     1081 cgtgaggtaa ttcgcatcga tggggggttg agggacggcc aggcgctcca cggccggctc
     1141 gaggaatccg gcacgggcaa ggccatcctt caccacgttg atgtctggga aacgggcctt
     1201 gtcgatcgcg gcaaagctgg ggaaataacg gttcatggga tgccgctcaa tgaactcgtg
     1261 cgaggccgtg atgaaagctg cgcaaccggt ccggagcacg cgggcacatt cgctggccag
     1321 ggcattcagg tcgtcaatgt agtgcagcat gtagcatccg aaacagagat cgaacgactg
     1381 cgcggcaaaa ggcaggtggc gggcatcggc gcgaagccag gcgccgggcg cccctttctc
     1441 tcgtgcccgc agaatcatgc cacgcgataa atcgcatgca aaaaatgagg cggcgggata
     1501 tgcggcgcga aatgcgaaca tgttgttgcc ggtaccggcg ccgacctcca ggatccggcg
     1561 ggcgtcatgc gcttgagcca gttcgaccag gaattcctga tacggccccc gccctccccg
     1621 aaacgaatca tagcgccgcg cgatctggtc ataatcgatc ttcgcaccgg cagccgggtc
     1681 gctcattccg cgggcgtctc ctttttttcg atctgcgtga agtcggcggc aacgctgatg
     1741 gcactgaaaa tggttttcag caatccgtgg cggttctgcc ggacggattt gtcgtcgtgg
     1801 ttgacgaaga cgtcttcgaa gtaggcctca atgacgggca tccaagacag gacttccgaa
     1861 aacgcggttt cgaacgcacc cgaggcgacc agatcgcgaa gccgggcgct gggctcgcct
     1921 gtcaacgtgt agagcgcctt ctcgttgact tccagcagcg cggagtcaat ggccggggcg
     1981 ggttccaatg cgatccgctg gatgcgtttg gtctgttcag cgaggagttg cacctgctgt
     2041 tcatccagac ggctgaacgc ctccaggcat ctgcgaacct ggacagggcg ccggccgtgc
     2101 gaggcgatag ccgcggcgac ctgatcgtag ggaatgcctt tctcgcggtg gataaccgcg
     2161 aggcggtcgg ccgcaaactg aagaaagacg tcgacggcgg cttcaggctc ggcaagaatc
     2221 ccatcataaa cgctcagggc ggaacggaat gcctcttcca aatcgatgtc caggccctct
     2281 tcgaggatga tggaaagaag agccagggtg ttgcggcgga gcgcgaaggg atcgctggtg
     2341 cctttgacgc ggatgcccaa accaaaataa ccggcgaggg tatcggtgcg gtccattacg
     2401 gcagccacga tccccagggg cgacttcgga agcctgtcgc ccgcgcgccg gggcagccgg
     2461 tgctcgaaaa gcgccagggc gatgtcttcc ggaagccctt ctttgcgcgc atagagcata
     2521 ccgaccacgc cctcgaggga atcaaattca aaaaccatct gcgtggtcag gtccgccttc
     2581 ataaggtcca gaacctgagc cacccgcgcg tcatcaaccg ccggcaggac gtttttgagc
     2641 atgggataga gccgatggag ccgggaaatt ttgtcggcga cggttccaag tttctggtga
     2701 aacaaaaccc gtgacaggcc cgccgcgaac tcacgcaggg gtttgcgcgt atcggtatcc
     2761 cagaaatacc gggcgtcgcg gaggcgcgcg ttgaggacgc gctcgtttcc gcggcggatg
     2821 gtggcacggc cttcctcgtc gtgcttgccg ttcgcgacgg cgaggaaatg cctgctgagg
     2881 cctccctggg catcccgcag ggggatgtag cgctggtgga ccttcatggg tgtgatgact
     2941 atctcttcgg gaagcgcgag agcgtcgtct ggaatgctgc acagaatggg ttcgggccat
     3001 tcggtgatgt cgatgatctc ctccaacaaa tcgctgtctt catcgagatg gagtccctgt
     3061 gcggatgcga tcgccccggc ttcactgcgc aaccgggcca tgcgttcgtc gaggacggga
     3121 agcacgaagt ggcgtttcat cgtctcgaga taatcgccgg ccgaagggat ggagacgggc
     3181 ccggccgcaa gccgccggtg gccgtacgac acggtgccgg aatggaggaa gcgctccgcg
     3241 gcaaattcac ccccatcgac gaacttccag gagactggca ccggattcgc gccgtggagg
     3301 gcgagcagcc agcggacggg acgcgagaac gtcaccgtgc tgtcttccca acccatggat
     3361 tttacaaact gcagaccggc gagcagtttc gtgacgatgc cggacaacag ctcagtaata
     3421 tgctgccctt tgcgctccac tttgatgaag caatactcgg ccttgccctg ctcctgaaaa
     3481 taaatgtcct cgacgcgcgc gcctttggat tccgcaaatt tccgcgcggg aacggtccac
     3541 tggccatcgg catccagcgc ggctttccgt gccggcccgc gagcttcttc catgagatcc
     3601 tgctgttgcg acgcgcagtc gtcaagccgg gcgacaatgc gccggggcgt cacccatacc
     3661 gtgacggggc cgtgttgaag gcgtgcttcc tggaacaatt tggtcatgag cgcgggcagc
     3721 tgggcgcgaa ccgccttgac gtcggaatga ggcaattctt cgaccccaat ctcgagcagg
     3781 agcgtgtcgc gctcctgaaa cgcgccggga tccgcctgca cgggcgacgc ttcagcttca
     3841 cgccatgggc gcggcagccg gcagagcgga aagccttttt cctgacgctg ttcaaagaac
     3901 cccttggcac agcgttccgc aaccgcccgg cagcggagaa gaaaccgccc gcgttcggtc
     3961 acggagagcg ccccccttgc atcgagcagg ttgaagagat gcgacaggcg gagacagtgg
     4021 tcgtacgcgg gcatgatgag ccccagttcg agcagccgtg ccgcttcggc ttcccagagg
     4081 tcgaacacct gaagcaactt gcccgtatcg gccgcctcga aattgtattt gcaccactcg
     4141 acctcggcgg cgtggtagat gtccccgtag gtgataccgg acggggccca tttcaggtcg
     4201 tatacgctct ccacaccctg aaggtacatc gcgatgcgct cgataccgta ggtgagttcg
     4261 caggcgcgca cgtcgagctt gaggccccca cactcctgga aataggtgaa ctggaggatc
     4321 tccatgccat cgagccaggc ctcccagccc agaccccagg cgcccaggga cgggctctgc
     4381 cagttgtctt cgacgaatcg ataatcgtgc cgggtaaggt ccaggccgag ggcttccaga
     4441 nnnnnnnnnn agagttcctg cacattgggc ggggatggct tcaggatgac ctggtactgg
     4501 tagtaatgtt gcagacggtt ggggttttcg ccgtagcggc cgtcggtggg ccggatgctc
     4561 ggctccggat aggcgaccca ccagggctcg ggacccagta cccgcagaaa ggtggcggga
     4621 ttcgaggtgc cggcgcccac ttcggtgtgg tagggctgcc agagaatgca gccgcgcgat
     4681 gcccagaagc tgttgagggt ttgcagtact tcctgaaccg ttatcacggg aaatctccct
     4741 gtatgcgctg cagtttgaaa acgggcccgc caggcgaagg cttcatgaat tcatgcggcc
     4801 cgcaggggcc ggaatcaaag gcggatttta gcgtttggaa gtgctccaag tcaaagcagt
     4861 ccgggttttt cgcgcctgat tttcggggct tggaggaggc tgcctgcacc atgggcggca
     4921 caagtctgag cggtattccg tgctacagac ggaggacgct gaacccgccg ccgacctcaa
     4981 tgaccgcgcc ggtagaatac ggaaaatggc ccccagcaat gctggcgact gctttcccga
     5041 catcttcggg ggtaccccag cgtttctgaa gcagcaggcc ttcggagatg agtttatcgt
     5101 atttttccga gacgggcgcc gtcatgtcgg tggcgatgat gccgggccgg atatcgtaga
     5161 cattgatgcc aaattcggcc agccgcactg caaaggtctg ggcggccatg ctgagtccag
     5221 ctttactgat acagtattcg gcacggtttg ggcttgcggt gttgctcgat atgctggtga
     5281 tgaagatgat gctgggcggg tctccgtccc ccgctgtcac ccgatcaatc atccggtttg
     5341 cgatcatctg cgttaagaaa aacggcccct tgaggttgat tcgcacgagc cggtcaaaac
     5401 tctcttctgt ggtctcaaga atgtccaggc gcttgagaag cccgacgccg gcgttgttga
     5461 ccagcaggtc gatgcggcca aacgcagcga aagcttcgtc caccaggcgg ttccgatcgt
     5521 ccatttcacc gatatcgccg gcaagcggga cgaaccgggc cccaagttct tccgctcggg
     5581 ctttgacttc aaacaggcct ttctccgcgt cttccggcgt aagccgggtc gcagtgccga
     5641 tgaggtcaaa cccgtttttc gccagttcga tggcaatgcc gcgtccgata ccgcggctgg
     5701 cgccggtaat catggctact ggtcgtcgca tggcttcctc gctggttgca tctggatttt
     5761 cggggcagcg ccgcggttca tccgtgttga acgcactata accatcgcat tcacatgcgc
     5821 gcaaggccac ggtggggcgc gaattgtccg cattgggggc ctgtggtatg ctgacggcga
     5881 aaaatgtgcg ttagctgagg aatctgtttt tttgacagcc gggcccggcc aggcgagaga
     5941 ggactgagac ccatgcaggc aacacggcgc gattttctgc ttcagacggg attcgggatt
     6001 gcagccactg gttttggact gtccagagcg atggcggagg acggaaagcc agcgctgcgt
     6061 ttgagcgcat gcgactggtc tctgggcgcc tgggggccag aagggctggc cctggcggca
     6121 gcggtagggc ttgatggcct cgagatctcg gccgcgacag acaatggcag ccccggtgac
     6181 gtcctggcgg tggcggatgc ggagcgccgc gcagcataca aagaacaatc caaagagcac
     6241 ggggttgtgg tctcgtcggt ggcaatgggc ctcttgaacg gttttccgat ggtcagcgac
     6301 ccgcgggcaa tggcctggac cgagcagacg atcgacggca cgaaggatct gggcgcaagg
     6361 gtcatgttga tggcgttttt tggcaagggc gacctgcgcg acaaatcggg ggcactcaaa
     6421 acgcaggacg tcgacaccct gatcggacag ctgaaggaac tggccccgaa agcggagaaa
     6481 gcgggcgtga tcctgggact ggagaacacc ctttccgcag agcagaatct ggacatcctc
     6541 gggcgcgtac aaagcaacgc cgtccgcgtc tattacgacg tggggaattc cacggccaat
     6601 ggatacgatg tgcccgccga gatcagcttg ctccgggacc gcatctgcca gatccatttc
     6661 aaggacaaca aaggccatct cggcgagggc gaagtggata tgtccgcggc agggagggcc
     6721 atgcgcgaca tcggatacgc ggggtgggtg gtgcttgaaa ccaaagtgct cggggaacgg
     6781 gacgcggatt tcaggcgcaa cgcggcgttt acccgggcct tgctcggaaa cgcctgacgc
     6841 cctttcccgg ccgtgtctga gcttgcacga gatgctattt tgcagcacgg ggcggattgt
     6901 gctaaaacta cgcccgtgaa aaactctaaa cgcttttggg cctcggcatg atccgcacgg
     6961 aacgcctccg aaaagtgtat tttcctcctc gatccaagcc ggtggaagga gtcaagaacg
     7021 ccacgttctc cgtggaaccg ggcgagatct ttggacttct cggacccaac ggcgccggca
     7081 agaccacgct tcttcgcatg ctgggcacga tcattgttcc gacatcgggg tactgctgga
     7141 tcgcgggcgt gcgcaccgat cagaaccccg acgcggtgcg gcaacatatt gggtttctgt
     7201 ccgggaacac gaaattatac gggcgcctga ccgcacgcga ggagttgcgc tatttcgggg
     7261 accttcacgg cctgccgaaa gaccggatcc ggcaacgcac ggaagaactg gccgagatcc
     7321 tggggatggg agaatttatc gaccgccgct gcgacacgct gtccacagga caaagccaga
     7381 aggtgtcgat tgcccgcgtc ttactgcatg atccggaagt gctgattctg gacgaaccga
     7441 ccacgggcct ggacatcatg acctgccaga ccattctgtc gtttattcga aacgcgaagc
     7501 actatggccg gagcatcctt ttttccaccc actacatgac cgaagcggaa atcttgtgtg
     7561 atcggctggc atttatttac agcggcgaaa ttcttgcaac aggcaccaag gagagcttgt
     7621 acgaaaaaac ggacaccacg aacctgaccg acgcgtttgt gcgcctggtc aatgccgcca
     7681 atacggtgcc ctcatgaaaa tgcgcacggt tcgcgccatc tttttcaaag aactgatggt
     7741 catgctgcgt gacaagcgaa cgctggtggc catgattggc atcccggtgg ttctctatcc
     7801 cgtgctgctt gtggtcacgt cacaggtggc gctcatgcac atatcccatg tggaagagtc
     7861 cacctcgcgc atcgccatca aggcgggaca agaccccacg gtcgcccact ggatcgatgc
     7921 gcttccccgc atgaagctcg tttcgatgga gaagccggaa gctgcgctgc tggacggcga
     7981 agtggatgcc gtggtcgtag tgaaacagcg ggcagaagag gtactggagc ggggcggctc
     8041 gttgctgatc gtcatccagt acgattcgac cgtcccgcaa tcgcgcgaag cggcccaccg
     8101 gctgagcgaa gggctccgca ctacacagac ccggctgctg gaagaccgcc ttgcgaaagc
     8161 ggggatcacg ctcgattttg ttacgccact caaacttcag gaaatgaacg tggcgcccgc
     8221 cgcgcgtatc gtcggcacac tgctcgccgc catgctcccg atgatcatgg tgctgatggt
     8281 ggggatcggg gccttttatc ctgcagtaga cctcacggcg ggcgaaaaag agcggggcac
     8341 ttttgaaacc ctgctctcga cgcccgcaac caagtttgag atcgtctgcg gaaaatttct
     8401 cacggtgttc tgcatcagca tgctgaccgg gctgctcaac ctggccagca tggcgatgac
     8461 gttcatattc catctttctg acctgctcac gcggctgcag gtgggcccgg agaaagcacc
     8521 cgtgcagttt cccctgggca ccgcgctgct gatgaccctg cttctcattc cgctggccct
     8581 gtttgtgtcg gccctgatga tgtcggtggc ggtgctggcc cgcaccttca aagaggcgca
     8641 gaactatgtc accccgttct tcctgctgat cacgattccg gcaggcctgg ccgggatgcc
     8701 cggtttggaa ctcaattctg tcctgcagtt tgtccccatc gtgaatatcg ggctcctgtt
     8761 tcgcgatttg ctcaccgacc gggcgaagct cgaggcggtg ttttccgttt tgttgagcac
     8821 gagtgccttt gctttgctgg cgcttgtctg tgcgacctgg ctgttccagc gggaagacgt
     8881 cattctctcc gaggagaaag gcgtgccgtt gaccctgcgc cgctcacgga ttgcaccacg
     8941 cggcgaacct acggtcggga tggccatgac cctgtacacg ctcatcctgc tgctgatctt
     9001 ttatgtgggg gtatatgctc agtcgaagca tatgctgggc ggtctgctga tcacggaatg
     9061 gctggtgatc gcgctgccta cggtggcgct gctctggtat ttgcgcgtgg atctgttgag
     9121 cgcgctgcat ctgcgggcgt tccgaattga cggggcgctg gcaaccgcag tgatcgcgtt
     9181 atcgtggctg gtgctcgcgt ttcaactggg gctctggcac agcaatgtgc tgccgatgcc
     9241 cgaggacatg gcaaaacagt acctccagct gatccatctg gaagatacca gtcttgtcgt
     9301 gctcctggcg gtcatcgcgg tctccccggc catttgcgaa gagatcctgt ttcgcggagc
     9361 gattctctcc gggctcgaga agcgtctgcc cgcctggctg ctcatccccg tggtgggtct
     9421 ccttttcggc gcattccatc tcagcattta ccgggtgctg ctgaccggcc tgacggggat
     9481 ggtgttgacc tacgtggtct ggcggacccg ctctctctgg aacggcatcc tgctgcattg
     9541 cctgttcaat gcatcggcgg tgctcatggc ctcggaacgg ctgcctgaaa agctgctcac
     9601 gggcctgcga aatttgtcag ccgcgcaggc cgggctgccg tggtggctga ttggcctggc
     9661 ggcgtcggcc atgtcggccg gcatcggcta cctgcacctg cggttccccg acccgaatcg
     9721 aggcccgtca gaataacagc tcgtttgcga gcatctggtc gagggtctgc cgtttccgga
     9781 tcaagtgcac ttccccgttc ttcctgagga gcacctcggc cgattcaggg aaggagttgt
     9841 agtttttgct gcacatggcg ctgcaatagg cgcccgcgcc cccaatggca agcgcatcgc
     9901 cgatgcgggg ttcaggcagg gcgcgcggaa gcagtccttc agggttcccc ggctcgggcg
     9961 tcagcaggtc tccgctttca cagcaatgcc cggcgatcag gtattcgccg cccggccgct
    10021 cttctccgga tgcggtaacc agggcgatgg ggtgctgagc gccatagagg gctggccgga
    10081 ggatgtcggt catcccggca tcggctttga taaaccggta cccctcgtta cctgtatcca
    10141 cgacatccat gaccgtcgtg atcagaacgc cggcattggc cacgatgaaa gtccccggct
    10201 caatctcgag tctcagttct tttccggtgg actcggcgta ttcgcggagg attttgctga
    10261 cggccccgcc gatctcctga aggtttgcag ccgactctcc gggcatgcgg gccaccttaa
    10321 atccgccccc gaggtttaat gtggccacct ccggaaagcg ggcggcggcc tccagggaaa
    10381 gcttcgcgca ttgggtccag acgctcggct ctccgcccga gccaatgtgc gtgtgaaccc
    10441 gggtgatatg gaggttcagc gcgctggcgg cgtcgaggac cttgggaatg tactcgtgcc
    10501 agattccaaa gctggatgag ggcccgccga cgttggtccg gttgttgtgc ccggagccga
    10561 gcccggggtt gatacgaacc cccagagacg ccccggggaa acgctcgccg aacgcgcgga
    10621 gctgtgccag ggaacaggca ttaaacagaa ccccagcctc gaccagttgc gtgaggttgt
    10681 gcgggagttc ctgggcggtc acttcaatat gttcaggcgg gatgccggcg cgcatggcgc
    10741 gttcggcctc atatccgctg ctggcgtcaa agtggagccc caaaccatgc aggagcctga
    10801 gtatggacag attcggcagg gctttcatgg cgaagcgggc agtcagcccg aacgggcatg
    10861 gaaactcaag tgcctggcgg gcctggtgct cgagcgtggc ctcgtcgtag acgtacaccg
    10921 gggtaccaaa ctcgcgctgg acggccagaa cctggttacg actcaaaaac agcgagggtt
    10981 tcatggatcg atactgcctt tctccgcctg gtgttcaaga acaggggctt atacatagca
    11041 cattcgcccc ccgttgaacc acctgccgat ggatccacgc tgcgcgataa ggccggcgtg
    11101 caggtgcacc gccgcgttga atccgggaga ctgcacgtgt gtatactgta ccaaacgggc
    11161 gggactggag aatgaggggg caaccgatga atcaacacga ggcgcgagag aaattcgccc
    11221 ggtccaaaat gctgttcgcn nnnnnnnnna agcgctatcc ggaagcgatg attgcgctgg
    11281 atgagctgaa tgccagcttc cccgacgaaa agcacgtcat gctggcacgg gccatgtgcc
    11341 tggccgagct ccagcggccg ctggaagcta tgcagatttg tgagcgggcg gtggcaatgc
    11401 accaggatcc caaggcggag gcgtacctgg cacgtttgca tcgagagcac ggtccactcc
    11461 agcacggccc gacagatctc gcgagcctgc tcgggcaggg caggaaagcc ggcgctcccg
    11521 gcgcagcggc ggggcttggc gcaatgcccg ctatcccgga tgtgccggat ctgacgattt
    11581 ataacccccc gcagcaatcc agcagtaaac ctttttacat cattctggga gccgtggctg
    11641 ccgttttggt ggtattgctg atcgggctgc ctctcctcct ctcgttgaaa aagaaaggcg
    11701 gtgaagcgcc tcagtccggt gcgatgacca ccgtggaaca ggcgacgccc acggaaaccg
    11761 gcgcacttcc cgatcccgca ggcacggagg ttcagccggt ggacgcagcc gccgcggcgc
    11821 caccagtcca gtggtacacc aactacgagc agggcacaaa cgttgccact gcgcacgggc
    11881 acccgatctg cctgtttttc agtgaggaca gcgaggcctc gcgcgcagtc gaaagctcgg
    11941 tgttcgaaga tcccggtatc aggcagtctt tgtcgcagtt catcaatatc cgtgtctccg
    12001 aggcggagaa ggagccggct gctgcgcagt acggcatcga gcaattcccg gctattgtcg
    12061 tagtgggcct cggcaacaat atcgtgtacc gggcgcaggg cgggaaaatt gatcccgcgg
    12121 aattacggtc ggccttgaaa ggcatgcaga tggcgcaggg ggccttcaag atgtggccct
    12181 ttactggagg catcccggta ttcgcggtag ttgtgatggg gctgcttagc ctgttgctca
    12241 ccccgtggcc tctctacctc acgctgttgt ttactggcaa gctgccccac gggcaatttg
    12301 tcaaagatgt cttctcggtc gctctggtcg cgatcggcgc caatgcggtt gctgggttcg
    12361 tcccctgcat gggcatcttc gtcctgattt ggattctaca tgcggtgtac gacctgggcc
    12421 ttgtcgattt tctgatctat tttgccctca gcacggttat gtgggtaata ctggcattga
    12481 ttacattcgc aaccatggga tcgacgatcc tgagtgcgtt gtccgaaata ccggccatgt
    12541 gatgggaaaa ccggggcggc tgcccggggc ttccctggtt tatccaaatc ttcgcagcgc
    12601 gtagcgcagg catcttgcct gcgttcttct cttgcgcagc gtaggtccgc gtgcctaccc
    12661 tgatccattc cggttttcgc agcgcgtagg ggcagacccc tgtgtctgcc cttcttcaaa
    12721 gcggccgcgt gggctatccg gatgggcggc cacagggggc cgcccctaca atgcagaaaa
    12781 cagctaaacg gcactttcac ctggactcac aaaacaggat gtctttgggg gaaaagcgcg
    12841 taaaagatgt gggttccatc ctcctgcggg catctgctat tctcttcgca gtatggcata
    12901 aggccagtgc acggcggatt ggcctgccgt tatctcaggg cacaaggagc cttcgcggga
    12961 atccctctat ggccaaaact cgatccacat ttgtctgtca aagctgcggc gccacgtaca
    13021 tgcgctgggt gggacgctgt acggattgcg ggcagtggaa caccgtgctc gaggaggtcg
    13081 tcgtcgaatc cggaaagcac ccccgcacgc ccactggaga ccagggggcg ccactaccca
    13141 tcactgatca gcagcacgaa cctccccccc gcctttcgac ggggatcctg gagtgcgacc
    13201 gggtactggg cggcgggatc gtccccggtt ccctgacgct catcgggggt gatccgggaa
    13261 tcggcaagtc aacgatgatg ctccagattt cgcatggact ggctcaggat gggggcccgg
    13321 tactctacat ttccggcgaa gaatcgttca gtcagtcgca gttgcgggcg cgccgcctgg
    13381 gcaccctatc cgacgagctg ttcatgctga cggagaccga gctggcctcc ctccgaaagt
    13441 atatccaggg cggcacattc agcttggtcg tggtggactc gattcaatcg gtctattcgg
    13501 ccgatctgca gggcgtgccg ggaagcatct cgcaggtacg cgattgtgcg agcgagtttc
    13561 tgcgcctggc caaaagctcg ggcacaccga ttttcctcat cgggcatgtc accaaggacg
    13621 gcacaatcgc agggccgcgc atgctggagc acctggtgga caccgtgttg tacttcgaag
    13681 gagaaggcaa gcaagcgctg cgaatccttc gggcggtgaa aaaccggttt ggctcgacca
    13741 acgagatcgg tgttttcgag atgaccgaga cgggacttca ggaggtcccc aacccgagcg
    13801 cgctgttcct gaacgagcgc cccgaaggcg tgagcggctc cgtggtattg cccagcgtgg
    13861 aaggcacccg tcccctgctg gtcgaggtgc aggcgctggt ctgtgattcg aacctgggct
    13921 cgccccggcg taccgtgacc ggagtgaatc ccaaccgggt atcgttgatc ctggccgttc
    13981 tggaaaagcg ggcgggactg catttcgctg atcgcgacgt gttcgtcaat gtggccgggg
    14041 gcgtgcgcct ggacgaaccg gccgcggacc ttgcgattgc cctcgcactc gtgtcgagct
    14101 ttatggaagc ccctgtcgct ccgcacctga ccgcctttgg agaaatcggc ctcgccggtg
    14161 agatacgcgg ggtggatatg gcgcgccggc gtgtcgcgga agctgccaaa ttcggcttta
    14221 cgcgctgcat tcttccaaaa aacaatgcca cggacgtgga aaacgcagat gaacaactct
    14281 ggcccgtcgg ccggattggc ccggcgatcg agctcgcgct ggaacgataa ccctgctcca
    14341 tgcgatgata cgcgggaaac gcagcgcaat agcgcggcac aggaaaacgc ggaatggtga
    14401 gaccaaaacg caaaacgaaa tccatagcct tccatgaggc gatccggatg atttcccccg
    14461 gcacgaagat ccgcgaagcc atctccatga tgctgcagag ccgcctcggt gcgctggtgt
    14521 gcatcggcga tcccaaaagg ctggccgacc tctccggcgg cggagtggaa ctgaatgccg
    14581 cgtgtacgcc gcagctgttg tatgaactgt ccaaaatgga cggggcgatc atactgaacg
    14641 aggacggttc caccatcacg tatgccaacc gcttcctgac gccgagcaac tcgattgtct
    14701 cgcaggaaac cggcacacgg cacatcgccg cggagcgcat ggccaagcag gcgaaatgcg
    14761 ccgtggtagc cgtgtcggaa cggcgttcga gcgtcaccct gtatgtgcag aatacacgcc
    14821 acgtcctgga cagcctcgcg acgctgctca acaagtcgtc gcaggcgctt caaacgctcg
    14881 aaaagtacat cagcagcctc aaccagtcga tggtggatct gagcacacgc gaatttgagg
    14941 atatggtgac gatttttgac gtgtgcaagg ccattcagcg gtgcgaaatg tcgcggcgaa
    15001 tcgcggctga aatcgagccg tacatcctgg aactgggcac cgaagggcgg cttatcgagc
    15061 tgcagttgcg cgaactctcc atccccattg aagaggcgcg cctcatcaca aaggactatt
    15121 acaaacaaaa gccctcggcc aacccggagg ccgtgcggca gcgggtcgag gaattgccgc
    15181 aggacgagct gatttccctg gaagccatca gccaggccct gggatacgga gccaaccttc
    15241 ggggtgtgga cacctatctt tcttcccggg gttaccgggt gcttgctcag acccgccgtc
    15301 tcacgccgca gattatcgag aacctggtaa accgcttcgg cacgctccag cagatcatgc
    15361 gggcgccaaa agatgagctg gtggctgtgg aaggcgtggg tgaagtcctg gcagaacgtg
    15421 tacggctcag cctgaacaat ctgcggagcc agctggccct ggatcgcggc aggacttgag
    15481 atgcggtata tccaggactg tgaagttgta acccacgacc aggctgcccc cagccactgg
    15541 cgcatgcggc tgcgttcgga ggacatcgcc cgagcggcaa aaccaggcca gttctgcatg
    15601 gtggaagtgg ccgaaaccct ctcccctttc ctgcgacggc ccatgtgttt ttcagagata
    15661 cacgatgacg gcttcacgtt gttgtacaag atagaaggcg aaggcacgcg gctgctttcg
    15721 cagcgggtcc cggggcagac ctgctcgata caggggccgc tgggaaatgg attccccatt
    15781 gttccggaga tcccgcggca catcatcgtg tcaggcggaa tcggcgtggc cccattcccc
    15841 gcgctggctc aggccctcac ggatgcatgc ggccggcctc ccgaggtggt tctcgcagcg
    15901 cgcaacaggg aactgctgct ctgtgcggag cagtttgaat cgttcggctg tacggtctat
    15961 ctggcgaccg acgacggttc ggcgggaaaa aaggcgtatg cagcccagat gctggagcgg
    16021 ttggcgcccg atcccgggac ccgtatttac gcgtgcggcc cgatggtcat gatgtcggcc
    16081 acggccgcgg tggccgcgca ggcaggggct tcatgcctgg tgtctctcga agcgcaaatg
    16141 gcgtgcggcg acgggacctg tctgagctgc gtggtcgagg cgaaagaaga gcgcgaaggc
    16201 gaaaaaatgg ttcgagtatg cgtggacggg ccggtgtttg acgcagcatt gatcgattgg
    16261 gaggcacaca gtacggccta tgacctctga tgccgcgaca ttggccgtca atctgggcgg
    16321 tctccgcatg aaaaacccgg taacggtggc ctccggcacc ttcgggtacg gtgaagagta
    16381 cggccggtac ttcgatgtgg cgcgtctcgg cgcggtaacg accaaaagca tctcgctcaa
    16441 accacgggcg ggcaacaaac cgccgcgcct ggccgagacg ccggcggggc tcctcaacgc
    16501 cattggcctc cagaacgtgg gcgtcgaacg atttctctcg cagaaggtcc cgtatctccg
    16561 taaatgcaac gccaccatca tcgcaaacat ctacgggcat tcgccggaag aatacgcggc
    16621 cctggcccgg cgtctggagg ccgaaggggc cgcggacgcc atcgaggcca acctctcgtg
    16681 cccgaatgtg cacgacgccc gatccgcccg gggagcggtg ctggtgtctc aggactgcca
    16741 ggccattacg gagtacaccc gggcgattcg cgaggcgacc cggctccccc tctacatcaa
    16801 gctgactccg aatgtcatga atatccagga accggccctg gctgcggaag cggccggagc
    16861 ggacggcgtc tgcctcatca acacgctgct gggcatggcg atcaatccgg agacacgaaa
    16921 accacgtctg gccaacattg tgggcgggct gagcggcccg gcgatccgcc cggtggccgt
    16981 caagatggta tgggatgccg cgcgcgtgct ccggataccc attatcggaa ccggcggaat
    17041 caccgacgcc gccagtgcga tcgaatttct tctggccggc gcgaccgcca tttcggtggg
    17101 ttcgatgtcg ttccgccagc cggatgccg
//
LOCUS       DAOPBQ010000013        17040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_3424_length_17040_cov_4.147424, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000013 DAOPBQ010000000
VERSION     DAOPBQ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 17040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17040
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3424_length_17040_cov_4.147424"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PKW60_01020"
     CDS             <1..567
                     /locus_tag="PKW60_01020"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HPJ98041.1"
                     /translation="GAPAAADSTGTALQNRAIRWGDAMDAARQLLVFQRLGGEFAFAN
                     FSNLANAWGQNVLECPKKGCYLSSTGRIFELFSAYPAAWPLRLEIDGEDPAVVAQAAW
                     DASRERLILIALNFHGEKAELSWDLSSLGLTRSQAELITLTAASPGSFNTMDQPDAIS
                     RSVEEKKRLGERVLFSLPPHSVMMAVTK"
     gene            723..3602
                     /locus_tag="PKW60_01025"
     CDS             723..3602
                     /locus_tag="PKW60_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPJ98042.1"
                     /translation="MPGQKKSISRRDFVGAVSRMTALGGGLYWMGTAAARTQAEASLK
                     RWGAAWRPASPQRLSAETHELAARAIAGEHGRSMVKAGVSLDESTMAALSPDIRYAKA
                     CQLVAEHAPLRILPGEKVVGSATLIEAAAHQTPVAGISSVSHTTLGFDRVLNEGYKGL
                     RRRIEERLARGGFDDSPSTPGFLADGPQGRIFCTSGQAAHWAAAAPRAEYDTPPLTVE
                     CRARVESKDGFNVLVLNRNKESRRHWELYSYAGSGCFSAYLPGHAPAEIKSERFIADG
                     AWHELAMTFDGARVALFVDGERVADEAVQARDVSDSEAGALYFGAYPPGNLGCHGAIA
                     EVRISNVVREIEPGEPWTADSHTIGLWRVGEMDGRPALTDASQLRNIAHFSPGPRTSE
                     DLLHSMLLCLDAAELWHQRHMDELARLAEASTGAERQHYLDVRETLRRVPENPPQTFR
                     EAVQSLWFMYAFQRLMGTWSGIGRLDAMLGPYLERDLAEGRITRDEARELLAHFWIKG
                     CEWIGAFETRGSGDAQHYQNIVLSGIGPDGTDVTNEVTWLVLDVVEELHISDFPIAVR
                     LNRNSPEQLLRRMAEVQRHGGGIVAAYNEEVAIDGLVKFGYPLNEARAFANDGCWETI
                     IPGKTCFIYRPFDGLSILQDALALRGGDPVDFPDFEALYAAWLERLAGAIDNHHRVAD
                     GWALNGIPTPLVSMFIDGCIESGRSYYDRGAVYTVFAAHIGRLANVANSLHVIKTLVY
                     EEQHLTLPEFLDIVRNDWDGQENLRRRVLNRSTFYGNDDDEADTMAIRVFNDYTDLMA
                     QVKERQGVLRPAGISTFGREIEWRNTGPSPDGHHRGELLATNCSPSPGTDREGPTAVL
                     KSYCKLDFTRSPNGATVELKLHPDSVKGETGIDAMVALSRTFVGLGGWFMHTDVVDSA
                     MLLDAQQHPENYPNLSVRIAGWSARFATLTRDWQDMVINRTQQRA"
     gene            3943..4596
                     /locus_tag="PKW60_01030"
     CDS             3943..4596
                     /locus_tag="PKW60_01030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPJ98043.1"
                     /translation="MAHPMLIIFDVDGTLLQSHRATVPAIREVFLAHGLTPPSEAAIQ
                     ETVGMPVHEYEAWLAQRCPQQTASAIVGAVNERELEFISTAGTLFPGVLQALQALKRA
                     GHALATCTNASVPYLARVLEEFSLRKFFQINTCIGQGYPHKTAMVRHIMNAIQLRPAV
                     VVGDRAGDIEAAKANGAYAIAACYGYGAEPEFHNADAHAHSVADIVDIVSRLGGMPL"
     gene            4675..6420
                     /locus_tag="PKW60_01035"
     CDS             4675..6420
                     /locus_tag="PKW60_01035"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HPJ98044.1"
                     /translation="MGFSIGPMKSGVGCLILFALPFIGVALFMGYLAATTVMAWHDMR
                     HWEEVPAFILEARLETSHDSDGSTYRATARYRYTYGGQEYTSERVAIATTSDNLGSFQ
                     QNIWRELAQYEGSETPFRCYVNPQKPSEAVLYRQLRPEMLLLYSVFVLMFGGAGVGMI
                     AAALHGHRRLRNEHRVRKLHPEQPWQWNNAWATGTITSSNKTVALFALLFAGFWNLVS
                     LVLTVLVVPESLRNGETAALLVLIFPAAGLVLAVWAAVSFLRWRKYGDSLFEMSAVPG
                     IVGGPLSGIIRAQARLQPDDGVNLTLNCIRRWTTGAGKNRSTEEKILWQDVCTVPRNQ
                     ITRDMAETVIPVHFAIPFDAEQTSDSTSGDRILWRLEASAATPGVDYRAQFEVPVFRT
                     PESRSDYATGEAFIESGHDAFDLEREVRAAGIVRTPKPSGGLRYNLTLARLKGAAAFL
                     TVFFVLWAGICVVLAVLGAPVLMTAIFCIIELALGVATFSLWFGSQELDVDFQVLTAT
                     SRFPGFARRKTFYYAEIEAITTCFGMQSGNRVYFNVEVKTSDGRVCPVAKYLPSRRLA
                     DAVAADLERALSGQG"
     gene            complement(6424..7755)
                     /locus_tag="PKW60_01040"
     CDS             complement(6424..7755)
                     /locus_tag="PKW60_01040"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HPJ98045.1"
                     /translation="MDWYYADGTNQVGPLDGRQMGQLAQQGKVTPETLVWRAGFPAWV
                     AAKKAAPHLFLGVAPQPAPPQAAAPKPGEGTPGAGRAPAPSLNLQSNLVDALADTGGA
                     PNLLNDLADFSGLSGLGVLGEAMSPVVADPVVGEEFFCAACGTMKPSSQAATFEGKVL
                     CLQCGRKLGKEEGLAYAEGEAFYREKAKRRPFRRLLAFYGRNRLQFIFAGAALGLLLV
                     IMLLRLARAFEPAGLLAFVLAAAVLGTAYTGNGLGRAYGRTVVLATIFALVGELFLFQ
                     GSGLLLIGALSFLAAHGAFLIAFWIRGFSLGWSMGTALPLLAISGASYFWLHTDASVE
                     MEIALIGFGLVLTTMTVFGIATVGADSGYLILLGAIGFYVSDLLFARALVFDLRAGLG
                     DTLCALPFRHVALLLFAFSISAEREQGIRLEEQRQAAAARAPQPYDPLIHF"
     gene            complement(7843..9120)
                     /locus_tag="PKW60_01045"
     CDS             complement(7843..9120)
                     /locus_tag="PKW60_01045"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HPJ98046.1"
                     /translation="MFPKANEALIEQAYFNAQEVYAQFGVDTAKALERLSAIPISLHC
                     WQGDDVAGFECIEAASGGGIQATGNYPGKARTPDELRQDAEKALSLIPGKHRFNVHAM
                     YAETGGKKVERNEIQPEHFQGWIDWAKANGLGMDFNPTCFAHPKADDGFTLSHHDKGI
                     REFWIEHCIACRKVGEHIGRSLGTPCVTNVWVPDGFKDIPADRLAPRARLKESLDAVF
                     ADAIEPKYNLDAVESKLFGIGSESYVVGSHEFYLGYALTNQKLLCLDAGHFHPTEGIA
                     DKISSVLLFLDEILLHVSRGVRWDSDHVVILSDDLRGIAEEIVRHDYLSRVHIGLDFF
                     DASINRVAAWVTGTRAMIKALLLALLEPRDRLLELELAGDYTARLALLEALKTLPFGA
                     VWDYYCRKLEVPAGCAWLNDVKQYEKDVLAARG"
     gene            9326..10030
                     /locus_tag="PKW60_01050"
     CDS             9326..10030
                     /locus_tag="PKW60_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPJ98047.1"
                     /translation="MRTQLAAVVPCYNAGGRVRAVVMQLKSLLERVYIVDDGSTDESA
                     QSVADLGGTILTFSENRGKGFALLAGFQAALELPETSAVAVVDADGQHDPDELPRLYA
                     AFVENQADLLIGARTLERDKVPWASWFGNSATALLTRVLLRRTIPDTQSGYRIHSRRF
                     LEDVLATVPGGRYETEMAIVSKAAREGYRLVSEPIRTIYEEDNRSSHFKKIRDSYRIY
                     RTFLLAALQPARTRKP"
     gene            10097..11104
                     /locus_tag="PKW60_01055"
     CDS             10097..11104
                     /locus_tag="PKW60_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPJ98048.1"
                     /translation="MIIPALGVLFLSVLPGLMACSPPKDSLLIYSPHGPDVLGDYKAK
                     FEAAYPGVTVQCLDMGSQEVYERIKGERNNPQADIWWGAPSSMFIQAAEDGLLAPYRP
                     SWADRVEAAFRDSEDRWYGTYLSPLAIVFNNRKHDKNTVPQTWDDLLLPEWRQKITLR
                     EPLPSGTMRTFICAMMLRAPSEDEGIAWLKRLDDATEAYMGNPQLLYDHLKRNEELIS
                     VWLLPDIALQRERNGYPLDTVLPPDTPVITEGIAIVAGAPHREWAERFYEFVTTTEAL
                     AQQAHAYAKVPARNDMDPAALPEWMSDLSIDAMEIDWRAFAEQERARINRWKTEVYGA
                     R"
     gene            11101..11841
                     /locus_tag="PKW60_01060"
     CDS             11101..11841
                     /locus_tag="PKW60_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98049.1"
                     /translation="MSSVQFQKVTRLFPEGGGIREFSCDIAEGEFFALLGPSGCGKTT
                     TLRIAAGLETPDSGRVAFDGHDVTDLPPEKRNAAMVFQSYALFPHMNVFENVAFGLRA
                     RKMPKDQLVSRVDESLALVQLAGLGKRAVAELSGGQQQRVALARALAVRPRILLLDEP
                     LSNLDAELRFSTRQQLAELQQRLGITAIYVTHDQEEALALAHRIAVLKDGICHQIGSP
                     DEILYQPATPFVQAFLERQRNAETKSRD"
     gene            12014..12805
                     /gene="ubiE"
                     /locus_tag="PKW60_01065"
     CDS             12014..12805
                     /gene="ubiE"
                     /locus_tag="PKW60_01065"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HPJ98050.1"
                     /translation="MPDSRFSRMSPDYVARKDYERMTDYVTSQSTVFPGRRDSWRMFD
                     RIVRRYDLLNRVLSFRLDQLWRRQVARMLPDRPGLRVLDLATGTGDMLFAIQDEKPGV
                     GHMMGMDMSAKMLSRGREKLRERPLHHAVQLVRSDATSIAAADGFFDAVTMAFGIRNV
                     EQMFTALQETRRVLRPGGRLLILEFSIPRGPFCQVYLFYLRHVMTRIGGYVSGDAAAY
                     EYLNRTIESFPCSETFCGLMREAGFHKVRACPQTLGIATIYSGER"
     gene            12812..14218
                     /locus_tag="PKW60_01070"
     CDS             12812..14218
                     /locus_tag="PKW60_01070"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HPJ98051.1"
                     /translation="MTAQQPHTVALDPGRARDWLASQICEAMKTASTASTAFPRSLRF
                     DAPVVHLNTLDWLRAQRAEHCVYWSGRDGHEQMAGVDAAHVLAGDEGHLESAFTAMQR
                     RLTPAHPRLRYYGGIAFDPAREPSAEWQPFGTYRFILPRFQLENRGPQSYLACNVFLR
                     SATDCTEECERTLAGLDALKFPENAAPAALPVPERRQDQPDSAAWEPLIAHALEAIRR
                     DSLAKVVLSRRSMFSFNVPLDPLALLMRMQARTPSSYAFCFRFGADAAFLGATPERLY
                     RRQGRRVASEAIASTRPRGATDAEDAALARDLLACDKDRREHAFVVRAIEEVFERLCR
                     SVDGARDISVLKLPTCQHLFCPLEGLLNDGVSDAALVQALHPSPAVGGVPTDRAVAYI
                     REHEPFDRGWYAGPVGWIGPESSEFAVAIRSGLVRGDTLSLYSGAGIVAGSTPDGEWD
                     EVETKLSNALSALTGNHD"
     gene            14211..15980
                     /gene="menD"
                     /locus_tag="PKW60_01075"
     CDS             14211..15980
                     /gene="menD"
                     /locus_tag="PKW60_01075"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HPJ98052.1"
                     /translation="MTDTAETLNPLWAGLLVEELVRNGVNTFCISPGSRSTPLTMAAA
                     QNARARCVVHFDERGAAYFALGYARTTEKPAALICTSGTAAANYLPAVIEADRSRIPL
                     LLLTADRPPELRETGANQTILQAGLFGSYTRWRFDLPCPTRDIPPAFVLTTVDQAVFR
                     ACGPDAGPVHLNCPYREPLVPGETAGGLEASISSPWGDSNAPYTAHAPVRLGLDPASA
                     NRLCVMIAEARRPVVAVGQLNRAADRAAAAALAALLRCPVFPDVASGLRLGRPDPSVM
                     THFDLFLSSPEIARELQPDLVIQLGGPMVSKSYQRWLGLHRPARCVRINSHPERDDPG
                     HLVSWRIQAALSQICEALAGCGTADNAPNKGVDAWFERVRDCQERSARALDRFFSAHH
                     DLIEPAVARYIAGAVEQSDGLFLGSSMPIRDAQRYASACGQGPVVFANRGASGIDGSI
                     ATVAGAAFGSGRTLTAIVGDLAALHDLNSLALLRTIKTPTVLVIINNNGGGIFHFLPV
                     SSQTAVFDPYFTAPHGLSFKDAAALFGLPYAKPDSMDDLKEAYTAALEQAGATLIEIT
                     TGRAENVALHKVIDEEVFKALQA"
     gene            16001..16849
                     /gene="menH"
                     /locus_tag="PKW60_01080"
     CDS             16001..16849
                     /gene="menH"
                     /locus_tag="PKW60_01080"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR03695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HPJ98053.1"
                     /translation="MARTLHYWMLNPAGEKTVVFLHGFMGSSEDWRPIAQGLPPNYRC
                     IAPDLPGHGRTPVRRDEAAYTMAAVSKDLMRLMDHIAPGPLALVGYSMGGRLALYLAL
                     EYADRVQALVLESASPGLRSETERAKRRVEDERRAVELESGDWEAFLRRWYAAPLFES
                     LHGCGDRFEELLMRRRNNNPRELARVLRGMGTGAQSSLWDRLSEAGMPTLLIAGERDA
                     KYCAMAAAMGAKMPRATAMVVSGAGHNVHFEKPADYTTLLRGFLDGLRGTAAQPPQHP
                     GTREQQ"
     gene            16849..>17040
                     /locus_tag="PKW60_01085"
     CDS             16849..>17040
                     /locus_tag="PKW60_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPJ98054.1"
                     /translation="MASIEWTSAGAFTDIRYEKAEGIAKITINRPEVRNAFRPLTVDE
                     MSAALSDARFDPEVGVIIPT"
BASE COUNT         3184 a         5363 c         5338 g         3155 t
ORIGIN      
        1 ggcgcgcctg ccgcagcgga ctccacgggg acggcgctcc agaaccgggc gatccgctgg
       61 ggcgacgcga tggatgctgc gaggcagctc cttgtgttcc agcgtctggg cggcgaattt
      121 gccttcgcca atttcagcaa tctggccaat gcctggggtc aaaacgtgct cgaatgtccg
      181 aaaaagggct gttacctgtc ctcaacgggc aggatattcg aactgttttc ggcgtacccc
      241 gccgcatggc cgcttcggct cgagatcgac ggcgaagacc ccgcggtggt ggcgcaggcc
      301 gcctgggacg cctcccgcga gcgtctcatc ctcattgcgc tcaattttca cggtgaaaag
      361 gccgagctgt cctgggatct gtcttcgctg ggcctcacgc gctcccaagc cgagctgatc
      421 acgctgaccg cagcatctcc cgggtcgttc aacaccatgg accagccgga tgccatttcc
      481 cgaagtgtgg aagagaagaa gcgtctgggc gaacgtgttt tgttttccct gcccccccac
      541 agcgtgatga tggccgtgac caaatagatg ccggccagac cgcatcccgg acagcgcggg
      601 caacagcctt gcagcatcgc caacaaccct gaaaatgcat tcagacaaaa ataccgctcc
      661 gccatcgtgt acaatcataa tgagttatga tgtccttaat cggaccggac aggagatgtt
      721 gtatgccggg gcagaaaaag agcatcagcc gcagggattt cgtgggcgcc gtatcgcgca
      781 tgaccgcgct gggcggcggt ttgtattgga tgggtacggc ggcggcgcgc acgcaggccg
      841 aggcttcatt gaagcgctgg ggtgcggcgt ggcggccggc ttcgccccaa cggctgtcgg
      901 ctgaaactca tgaactggcg gcgcgggcca ttgccggcga gcacggccgt tcaatggtaa
      961 aagcgggtgt ctcgctcgac gagagcacga tggccgcact gtcgccggac atccggtacg
     1021 cgaaagcctg tcaactggtc gcggaacacg cgccgctccg cattctcccc ggtgagaaag
     1081 tcgtgggttc ggctacgctg attgaggccg cggcgcatca gacgcccgtg gccggcattt
     1141 caagcgtcag ccacaccacc ctggggttcg accgggtgtt gaacgaaggg tacaaggggc
     1201 tgcggcgccg cattgaggag cgcctggccc ggggcggctt tgatgactcg ccttccacgc
     1261 caggattcct ggcagacggc ccgcagggcc ggattttctg caccagcggc caggccgcgc
     1321 actgggccgc ggcagctccc cgtgcggaat acgatacgcc gccgctgacc gtggaatgcc
     1381 gtgcccgggt tgagtccaag gacggattca acgtcctggt gctgaatcgc aacaaagaga
     1441 gccgccgaca ctgggaactg tattcgtacg ccggttcggg ctgtttcagc gcgtatctgc
     1501 ccggtcatgc ccccgccgag atcaaatcgg agcggtttat cgccgatggc gcctggcacg
     1561 agcttgccat gacgttcgac ggggcccggg tcgccctgtt tgtggacggc gaacgcgtcg
     1621 ccgatgaggc ggttcaagcc agggacgtgt ccgattcaga ggctggcgcg ctgtattttg
     1681 gcgcatatcc gcccggaaac ctcggatgcc acggcgcgat cgcggaagtg cgcatctcca
     1741 atgtggtgcg tgaaattgag ccgggcgagc cctggaccgc ggacagccac accatcggtc
     1801 tctggcgcgt tggcgagatg gacggacggc ccgccctcac agacgcgtcg cagctccgca
     1861 atatcgccca tttcagcccg ggcccgcgca ccagcgagga cctgctgcat tccatgctgc
     1921 tgtgcctgga cgcggcggag ctgtggcatc agcgtcacat ggacgagctg gcccgcctcg
     1981 cggaggcctc cacgggcgcc gagcgccagc attatctcga cgtgcgcgaa accctgcgcc
     2041 gcgtgcccga aaatccacct cagacgttcc gggaggccgt gcagtccctg tggttcatgt
     2101 acgcgttcca gcggctgatg ggcacctggt cgggcatcgg ccgcctggac gccatgctgg
     2161 ggccctatct tgaacgcgat ctggccgaag ggcgcatcac gcgggatgag gcgcgcgaac
     2221 tgctggccca tttctggatt aagggctgcg aatggatcgg cgcatttgaa acccgcggct
     2281 ccggcgacgc ccagcattac cagaacatcg tgctcagcgg gattggcccc gacggcacgg
     2341 acgtcaccaa cgaggttacc tggctggtcc tcgacgtggt cgaggagctg cacatcagtg
     2401 acttcccgat tgccgtacgg ctcaatcgca attcacccga gcagctgctt cgccgaatgg
     2461 cagaggtgca gcggcacgga ggcggcattg tcgccgcgta caacgaggaa gtcgccatcg
     2521 acgggctggt caaattcggc tacccgctta acgaggcccg cgcgttcgcc aacgacggct
     2581 gctgggagac catcatcccc ggcaagactt gtttcattta ccgtcccttt gacggcttga
     2641 gcattctgca ggatgcgctt gccctgcgcg gtggcgatcc ggtggatttt ccggatttcg
     2701 aagccctgta cgccgcgtgg ctcgagcgcc tggccggagc catcgacaac caccaccgtg
     2761 tggccgatgg ctgggctctg aatggcatcc ccacgccgct cgtgtccatg ttcattgacg
     2821 gctgcatcga gtccggccgt tcctattacg atcgcggcgc ggtgtacacg gtatttgcgg
     2881 cgcatatcgg gcgcctggca aacgtcgcca acagtctgca cgtaatcaag acgctggtgt
     2941 acgaggagca gcacctgacg cttcccgaat ttctggacat cgtgcgcaac gactgggacg
     3001 gccaggaaaa ccttcgccgc cgcgtgttga accgttcgac cttctacggc aacgacgacg
     3061 acgaagccga caccatggcc atccgcgtgt ttaatgacta cacggatctc atggcacagg
     3121 tgaaagagcg ccagggcgtc ctgcgcccgg cgggcatcag caccttcggc cgcgaaatcg
     3181 agtggcggaa caccggcccg tcgcccgacg gacaccaccg gggcgagctt ctggcgacga
     3241 actgttcgcc gtcgcccgga acggaccggg aagggcccac ggctgtcctg aaatcgtatt
     3301 gcaaactcga ttttacccgc agccccaacg gtgcgaccgt ggaactcaaa ctgcatccgg
     3361 acagcgtcaa aggagagacg ggcattgacg ccatggtggc gctgtcgcgg acttttgtgg
     3421 gcctcggcgg ctggttcatg cacaccgacg tggtcgactc cgcgatgctc ctggatgcac
     3481 aacagcaccc cgaaaactat cccaatctat cggttcgcat tgcgggatgg tcggcccgtt
     3541 tcgccaccct gaccagggac tggcaggaca tggtgatcaa ccgcactcag caacgtgctt
     3601 aacaagagag cagccgccgg ttttgcgatg gcgggccggc ggcaggctgc tgaaatcagc
     3661 cgaaaacacc gtttcacaaa acgggttctc ggccgcatgg ggcaacgggt gccgggtatc
     3721 gatcctgccc acaaatgccc gcttttcatc gaacccgcgc gtcaattccg ctggcgtcaa
     3781 ctcggaatgc ttacgctgaa cgggatggag tgcaacactc ctcctcttcg gtgagatcgg
     3841 ggattatgtc gcgcctggtg ccgcaggaat gatagcctcg ccctgtgtaa acgtgcggta
     3901 cttcaacact ggaattgaag accggtgttt caggaggaaa tcttggcgca tcccatgctc
     3961 atcattttcg acgtcgacgg aacgctgctg cagtcacatc gggcgaccgt accggccatc
     4021 cgcgaagtct tcctggccca tggcttgacg ccgcccagcg aggcggccat tcaggagacc
     4081 gtcggcatgc cggtgcacga gtatgaggcc tggctggcgc aacgctgccc gcagcaaacg
     4141 gcctccgcca tcgttggcgc cgtcaacgag cgggaactcg agttcatttc tacagccggg
     4201 acactctttc cgggcgtttt gcaggccttg caggcactga aacgggccgg acacgccctg
     4261 gcgacttgca ccaacgcgtc cgttccctat ttggcccggg tgctcgaaga atttagcctg
     4321 cgaaaattct tccagattaa cacgtgcatc ggccagggct acccgcacaa gaccgcgatg
     4381 gtgcgccata tcatgaacgc catccagctg cggccagccg ttgttgtggg cgatcgcgcc
     4441 ggcgacatcg aggccgccaa agccaacggc gcgtatgcca tcgcggcctg ctatggatac
     4501 ggcgccgaac ctgaattcca caatgcggac gcccacgcgc acagcgtggc cgatatcgtg
     4561 gacatcgtgt cgcgcctggg cggcatgcca ctctgagtct gtctccgcca tttttctccc
     4621 gggccgccgc aggtatacta cgatccggaa gcactcaggg cggaggctga ttacatggga
     4681 ttctccattg gcccgatgaa gagcggcgtg ggatgcctca tcctgttcgc ccttcctttt
     4741 attggggtcg ccctgttcat gggctacctg gcggcaacga cagtgatggc ctggcacgac
     4801 atgcgccact gggaggaagt gcctgcattt atcctggaag cccggctcga gacctcccat
     4861 gattccgacg gttccaccta ccgggccacg gcccgatacc gctacaccta cggcggccag
     4921 gaatacacca gcgagcgcgt ggccatcgcg actacgtcgg acaacctcgg ctcctttcaa
     4981 cagaacatct ggcgcgaatt ggcccagtac gaaggatcag aaacgccatt ccggtgttac
     5041 gtgaatcctc aaaagccgtc ggaggccgtg ctgtaccgcc agctccgccc ggaaatgctc
     5101 ctgttgtact cggtatttgt tctgatgttc ggcggcgccg gcgtggggat gatcgccgcg
     5161 gcgctgcatg gacaccgccg ccttcgcaac gaacaccgtg tccgcaaact gcatccggaa
     5221 cagccgtggc aatggaacaa cgcctgggcc accgggacaa tcacctcgtc caacaagacc
     5281 gtcgctcttt tcgcgctcct gttcgcaggt ttctggaatc tggtgtccct ggtgttgacc
     5341 gtgctcgtcg tgccggaaag cctgcgcaac ggtgaaacgg ctgcgttgct ggtgctcata
     5401 tttcccgcgg caggcctggt cctggccgtc tgggcggcgg tgtcgtttct ccgctggcgg
     5461 aaatacggcg attctctgtt tgaaatgtcg gcggtgcctg gaatcgtcgg cggcccgctc
     5521 tccggaatca tccgcgcaca ggcccggctt cagccggacg acggagtcaa cctcacgctc
     5581 aactgcatcc gcagatggac caccggggca ggcaaaaacc gcagcacgga ggagaagata
     5641 ctctggcagg atgtctgtac ggtgccgcgc aaccagatca cgcgagacat ggccgagacc
     5701 gtcattcccg tccacttcgc catccccttc gacgccgaac agaccagcga cagtacctct
     5761 ggcgatcgca tcctctggcg attggaagcg agcgccgcca cgcccggcgt cgattaccgc
     5821 gctcagtttg aggtgccggt ttttcgcact ccggagagcc gttcggatta cgccaccggc
     5881 gaagccttca tcgaaagcgg gcacgatgcg ttcgacctgg aacgggaagt gcgggcggcg
     5941 ggcatcgtcc gcacgcccaa gccgtctggc ggactgcgct acaatctcac cctggcccgg
     6001 ctgaagggcg cggcagcctt tctgacggtt ttcttcgtgc tctgggcggg catctgcgtc
     6061 gtgctggccg tgctcggtgc gcccgttttg atgacggcca tcttttgcat catcgaactg
     6121 gcgctcggcg tggcgacgtt cagtttgtgg ttcggctcgc aagagttgga cgtggatttc
     6181 caggtgttga ccgcgaccag ccgttttccg ggcttcgccc ggcggaagac tttctattac
     6241 gccgagattg aagccatcac aacgtgtttc gggatgcaat ccgggaacag ggtgtatttc
     6301 aacgtggagg tcaaaacgag cgacgggcgg gtctgtccgg tagcaaaata cctccccagc
     6361 cgcaggcttg ccgacgctgt cgcggcggat cttgagagag ccctgtcggg tcaggggtaa
     6421 tcctcaaaaa tgaatcagcg ggtcataggg ctgcggagcc ctggcggcag cggcttgtct
     6481 ttgctcctcc agccggatgc cctgctcgcg ttccgccgaa atgctgaagg cgaacaagag
     6541 cagcgcaacg tggcgaaaag gaagcgcgca gagcgtgtcg cccaggccgg cgcggagatc
     6601 gaagaccagg gcgcgggcga acagcaaatc ggacacgtag aagccaatcg cgcccagtag
     6661 aataagatag ccagaatccg cgccaacggt cgcgataccg aagacggtca tggtcgtcag
     6721 caccagccca aatccaatca gggcaatctc catctcaacg gaggcgtcgg tgtggagcca
     6781 gaaatagctc gccccgctga tggccaacaa cggaagtgcc gtccccatgc tccagcccag
     6841 cgaaaacccc cgtatccaaa acgcaatgag aaaggcgcca tgcgcagcga gaaaagacag
     6901 tgccccgatc aggagcaatc cgctcccctg aaacagaaac agttccccaa ccagcgcgaa
     6961 aatcgtggca agcacgacgg ttcgtccgta ggctcgcccc agcccgtttc cggtataggc
     7021 cgtgcccagc accgccgccg ccaggacgaa tgcgagaagg cccgcgggct caaaagcgcg
     7081 cgccagacgc aacagcatga tcacgagcag gaggcccagc gccgcgcctg cgaaaatgaa
     7141 ctgaaggcgg ttgcgcccgt aaaaggccag caaacgccga aacgggcgtc gcttggcttt
     7201 ttccctgtag aacgcctctc cttcggcgta tgcgagacct tcctccttcc ccagcttgcg
     7261 gccgcactgc aggcacaaga cttttccttc aaaagtggcg gcctgcgagc tgggtttcat
     7321 ggtgccgcag gcggcacaga aaaactcttc ccccaccacc ggatcagcga caacggggct
     7381 catcgcctcg cccagcacgc ccaggccgct gagtccggag aaatcggcca gatcgttgag
     7441 caaattcggc gctccgcccg tgtcggccag cgcatcgaca aggttgctct gcaagttaag
     7501 cgaaggggcc ggtgcgcgcc cggcgcctgg agtgccttcc cccggttttg gcgcggcagc
     7561 ctgaggcggg gcaggttgcg gcgcaacccc aagaaacaag tggggagcgg cctttttcgc
     7621 agccacccac gctggaaatc ccgcgcgcca caccagcgtt tccggggtga ctttgccctg
     7681 ttgggccaat tgccccatct ggcggccgtc cagaggtcct acctgattcg tcccgtccgc
     7741 gtagtaccag tccataccat cctctcgacg cattgccggc gcgcagcatg ctggccgggt
     7801 catgaaaagg gccggcctgg cggcctgtgt cacggtctgg gactaccctc gggccgcgag
     7861 cacgtccttc tcatactgtt tcacgtcgtt gagccaggcg cagccggccg gaacctcaag
     7921 tttccggcag taatagtccc acaccgcacc aaatggcaac gtcttcaacg cttcaagcag
     7981 ggcaagccgg gccgtgtaat ccccggcgag ttccagttca agcagtctgt cgcgcggctc
     8041 gagcagcgcg agcagcaagg ctttgatcat agcgcgtgtg ccagtgaccc acgcggcaac
     8101 gcggttgatg ctggcatcaa agaaatccag gccgatgtgc acccggctca gatagtcgtg
     8161 gcgaacaatc tcctcggcaa taccgcgcaa gtcgtcggag agaattacaa catggtcact
     8221 gtcccagcgg acgccgcggc tcacgtgcag caggatctcg tcgaggaaca gcaataccga
     8281 tgagatcttg tcggcgatgc cttcggtggg atgaaaatgg cccgcatcca ggcacagcag
     8341 cttctgattc gtgagggcgt agcccaggta gaactcgtgc gaacccacca cgtaggactc
     8401 ggacccgatc ccgaagagct tcgactcgac ggcgtccaga ttgtatttcg gttcaatggc
     8461 gtccgcgaac accgcgtcga gcgactcttt caggcgggca cggggcgcga gccgatcggc
     8521 cgggatatct ttaaacccat ccgggaccca cacgttggtg acgcacgggg tacccagtga
     8581 acggccgatg tgttccccca ccttccggca tgcgatgcag tgttcaatcc agaactcgcg
     8641 gatgccttta tcgtggtgtg acagggtgaa gccgtcgtcg gctttcgggt gggcgaaaca
     8701 ggtggggttg aaatccatgc ccaacccgtt tgcttttgcc cagtcgatcc aaccctgaaa
     8761 atgttcgggc tgaatctcgt tgcgctccac ttttttaccg ccggtttcgg catacatcgc
     8821 gtgcacgttg aaccggtgct tgccgggaat gaggctcaag gctttctcgg catcctgccg
     8881 gagttcatcg ggcgtccgcg ccttgccggg atagttgccc gtcgcctgga tgccgccgcc
     8941 ggacgctgcc tcgatgcact cgaaaccggc cacgtcgtcg ccctgccagc agtgcaggga
     9001 aatggggatc gcgctcaggc gctccagggc cttcgccgtg tccacgccga attgcgcgta
     9061 gacttcctgc gcgttgaaat aggcctgttc gatcaatgct tcgttcgcct ttggaaacat
     9121 ggttcttccc ttctgcacgg ctcggactga ctgctgcttc ccagactctc agaaatgcat
     9181 tatacaggaa cgccatgagc tcgcggcaaa cccgccagca cggcgctgcg aggcagcagg
     9241 cgccctgcgg ccgggccgat tgccgcaagc gggttgcgcc gctacaatac gggccatgga
     9301 cgacgctgga caggatacaa gcgccatgcg gacgcagctg gccgcggtgg tcccgtgcta
     9361 caacgcgggc ggccgcgtcc gggccgtggt catgcagctc aagagtctgc tggagcgggt
     9421 ctacatcgtg gatgacggaa gcacggacga atcggcgcag tccgttgctg atttgggcgg
     9481 caccatcctg actttcagcg agaaccgcgg caaaggattc gccctactgg cggggttcca
     9541 ggccgcgctg gagctgccgg agacatcggc ggtggccgtt gttgatgccg acggccagca
     9601 cgatccggat gagctgccgc gcctgtatgc ggcgtttgtt gaaaatcagg cggatctgct
     9661 catcggcgcg cgcacgcttg aacgcgacaa agtcccgtgg gcgagctggt tcggcaattc
     9721 ggctacggcg ctcctgacgc gcgtgctgct tcgccggacc atccccgaca cgcaatcggg
     9781 gtaccgcatc cattcgcgcc ggttcctgga agacgtcctg gcgaccgttc caggcgggcg
     9841 ttacgagact gaaatggcca tagtgagtaa agcggcgcgg gaaggttacc ggcttgtctc
     9901 cgaacccatc cgcaccatat atgaggaaga caatcgttcg tctcatttca agaagatacg
     9961 cgattcgtac cgtatttacc gcacattcct gctggctgcg ctgcagccgg cccgaacaag
    10021 gaaaccttga gtgaattgca tgcctgccca cgcgatgccg tcaaagcttg ccgcacaaaa
    10081 agccagagcc ggatcggtga tcattcctgc acttggcgtt ctgtttttga gcgtgctgcc
    10141 cggcctcatg gcctgctctc cgccgaaaga ctcactgctg atttacagcc cgcacggccc
    10201 tgatgtgctg ggtgattaca aggcgaaatt cgaagcggcg tatcccggcg tgaccgtgca
    10261 atgcctcgat atgggctcgc aggaggtcta cgaacggatc aaaggcgagc gaaacaatcc
    10321 ccaggccgac atctggtggg gcgcgccctc gtcgatgttc atccaggcgg ctgaagacgg
    10381 cctgctcgcg ccgtaccgtc catcatgggc ggatcgcgta gaggccgcat tccgggacag
    10441 cgaagaccgg tggtacggaa cctatctttc cccgctggct atcgtgttta acaaccggaa
    10501 acacgacaaa aacaccgtgc cgcagacctg ggacgacctg ctcctgccgg aatggcgcca
    10561 gaagataact ctccgcgaac ctttgccctc gggcaccatg cggaccttca tctgtgcgat
    10621 gatgctgcgc gctccgtcgg aagatgaggg gatcgcgtgg ctcaaacgcc tcgatgatgc
    10681 taccgaagca tacatgggca acccgcagct cctgtacgat cacctcaaac ggaatgagga
    10741 gttgatcagc gtctggctgc tgcccgacat cgccctccag cgtgagcgca acggctatcc
    10801 gctcgacacc gtcctgccgc cggacacgcc ggtgattaca gaaggcatcg cgatcgtcgc
    10861 cggggcgccg caccgtgaat gggccgagcg tttctacgaa ttcgtcacga ccaccgaggc
    10921 cctggcgcag caggcccacg cctacgccaa agtgcccgca cgaaacgaca tggatcccgc
    10981 cgcgctgccg gaatggatga gcgatttgag catcgacgcc atggaaatcg actggcgcgc
    11041 gtttgcggaa caggaacgtg cgcgcatcaa ccggtggaaa actgaggtct acggcgcccg
    11101 atgagcagtg tgcaatttca gaaggttacc cggctctttc ccgaaggcgg cggcatccgc
    11161 gaattttcct gcgacatcgc ggaaggggag tttttcgccc ttctgggtcc gagcggatgc
    11221 ggcaaaacaa ccacactgcg cattgccgcc ggtctcgaaa cgcccgattc cggaagagtc
    11281 gcgtttgacg ggcatgacgt cacggatctc ccgcccgaga agcgcaacgc ggccatggtg
    11341 ttccagagtt acgccctctt tccccatatg aacgtgttcg aaaatgtcgc atttggattg
    11401 cgcgcgcgca aaatgcccaa agatcagctc gtttcgcggg ttgacgagtc gcttgctctg
    11461 gtgcagcttg cgggcttggg caaacgcgcc gtggccgagc ttagcggagg tcagcagcag
    11521 cgggtggcac ttgcacgcgc gctggccgtg cgcccacgca ttcttcttct cgacgaacct
    11581 ctgagcaacc tcgacgccga gctgcgtttc agcacgcggc agcagcttgc cgaactccag
    11641 cagcggctcg gcatcaccgc catctacgtc actcacgacc aggaggaagc cctcgccctg
    11701 gcccaccgga tagccgtgct caaagacggc atatgccacc agatcggttc gccggacgaa
    11761 attctctacc aacccgcaac gcccttcgtg caggccttcc tcgagcgcca acgcaatgcg
    11821 gagaccaaat cccgggactg agccagccgg gacggtaagg cagcgcgagg atcgtttcgg
    11881 aagagatgtc tgcgcggcgg tgttagtttc ttgagaccac aggctgcgca ccgatagacg
    11941 aatacaggcg aattttacat ttctgcgctt ccatgcgaca attccctgtc ttctcccgcc
    12001 ggaagagccc gccgtgccgg acagccggtt ctcgcggatg agcccggact acgtcgcacg
    12061 caaggattac gaaagaatga ccgattacgt gacttcacaa agcacggtat ttcccggacg
    12121 ccgggacagc tggcgcatgt tcgatcgcat tgtgcgtcga tacgacctgc tgaaccgcgt
    12181 gctgtcgttc cggttggacc agctgtggcg ccggcaggtg gcgcggatgc tgcccgacag
    12241 gcccggcctg cgcgtcctcg atctggctac aggcacggga gacatgctct tcgcaatcca
    12301 ggacgaaaag ccgggcgtcg gccacatgat gggcatggat atgtcggcga aaatgctgtc
    12361 gcgcggaaga gaaaagcttc gcgagcggcc gcttcatcat gccgtgcagc tggtgcgatc
    12421 ggacgcgacc tcgatcgccg cggccgacgg tttttttgat gccgtgacca tggcgtttgg
    12481 cattcgaaat gtggagcaga tgtttaccgc gttgcaggaa acgcggcggg tgctgcgtcc
    12541 tgggggccgc ctgctcatcc tcgaattctc gattccacgg gggccattct gccaggtgta
    12601 tctgttctat cttcggcacg tgatgacgcg catcggggga tatgtgtccg gcgatgcggc
    12661 ggcctacgaa tacctgaacc gaaccattga gagtttcccc tgctcagaga cattttgcgg
    12721 cctgatgcgc gaggcgggct tccataaagt gagggcctgc cctcagacgc tggggattgc
    12781 cacgatctac agtggagagc ggtaggctgt catgacggcg caacagccgc acacagtggc
    12841 gctggacccc ggccgcgccc gggactggct ggcgtctcag atatgcgagg cgatgaagac
    12901 cgcctccaca gcgtccacgg cctttccccg gagcctgcgt ttcgacgcgc ctgtggtgca
    12961 cctgaatacg ctcgactggc tccgtgccca gcgagcggaa cattgcgtat attggtctgg
    13021 acgcgacggc cacgagcaga tggccggcgt ggacgctgcc cacgttctgg ccggggatga
    13081 gggccacctc gaatccgcat ttacggccat gcagcgccgc ctgacaccgg cgcatccccg
    13141 cctgcggtac tacgggggaa tcgcctttga ccccgcgcgg gaaccttcgg ccgaatggca
    13201 gccgtttggc acgtaccggt ttatcctccc gcgctttcaa ctggaaaacc gcggcccgca
    13261 gtcgtatctg gcgtgcaacg tgtttttgcg gtcagccacg gactgcacgg aagagtgtga
    13321 acgtacgctg gccggtctcg acgcactgaa atttcccgaa aacgcagctc cggcggccct
    13381 gcccgttcca gaacgccggc aggatcaacc cgattcagcc gcctgggaac cgctgatcgc
    13441 gcatgcgctg gaagccatcc gccgagacag cctcgcgaag gtggttcttt cacggcgatc
    13501 catgttctcg ttcaacgttc cgctggatcc gcttgcactg cttatgcgga tgcaggcccg
    13561 gacgccttcc tcctacgcgt tctgttttcg gttcggcgcg gacgccgcgt ttcttggcgc
    13621 gacacccgag cggctctacc gccggcaggg caggcgcgtg gcgagtgagg cgatagccag
    13681 cacccggcca cgcggggcca cggacgccga ggacgcagcc ctcgcgcgcg atctcctcgc
    13741 atgcgataaa gaccgccgcg aacatgcgtt cgtcgtgcgc gcaattgagg aggtatttga
    13801 gcggttgtgc cggagcgtag atggtgcgcg ggacatttcc gtgctgaagc ttccgacctg
    13861 ccagcacctg ttctgccctc tggagggcct gttgaacgac ggcgtgtcgg atgcggccct
    13921 ggtgcaggca ttgcatccaa gccccgcggt gggtggcgtt ccaacggaca gggcggtcgc
    13981 gtatattcgc gagcatgagc cgttcgaccg gggatggtac gctgggccgg tgggttggat
    14041 tggaccggaa tcgagcgagt ttgccgtggc gattcggtcg gggctggtgc gcggcgacac
    14101 gctgtcatta tactcggggg cgggcattgt cgcgggttcc acgccggacg gcgaatggga
    14161 cgaggtcgag accaaactga gcaatgcgct gagcgcactc accggaaacc atgactgata
    14221 ccgccgaaac gctgaatcct ctctgggccg ggctgctggt cgaggaactg gtgcgtaacg
    14281 gcgtcaacac gttttgcatc tcacccggtt cccgctccac tccgctcacg atggccgctg
    14341 cacagaatgc ccgcgcgcgg tgcgtggtgc attttgatga gcgcggcgcg gcgtatttcg
    14401 cgctggggta cgcccggacc accgaaaagc cggcggcgct gatctgcacc tcgggaacag
    14461 ccgccgcgaa ttacctgccg gcggtcatag aagcggaccg ctcgcgcatc cccctgctcc
    14521 tgctgaccgc ggaccggcct cctgaattgc gggagaccgg cgcgaaccag accattctcc
    14581 aggcgggcct gtttggctca tacacccgct ggcgtttcga tttgccgtgc cccacccggg
    14641 acattccccc ggcgttcgtg ttgaccacgg tcgatcaggc cgtcttccgc gcctgcggac
    14701 cggatgcggg ccccgtccat ctcaactgcc cctaccgcga accgctcgtc cccggagaaa
    14761 cggccggggg gctggaggcc agcatctctt cgccgtgggg tgactccaac gcaccttata
    14821 cggcgcatgc acccgtccgg ctgggcctcg atccggcgtc ggcaaaccgg ctgtgcgtaa
    14881 tgattgcgga agcgcgccgg cccgtcgtgg ccgtaggcca attgaatcgc gcggcggatc
    14941 gggctgcggc tgcggcgctg gccgctctgt tgcgatgccc cgtctttccg gatgtggcct
    15001 ccggactccg cctggggcgt ccggatcctt ccgtgatgac ccattttgac ctgtttctct
    15061 cctcgccgga aatcgcgcgg gaacttcagc ctgatctggt aattcagctg ggcggtccca
    15121 tggtttcaaa gagttatcaa cgctggctgg ggttgcatcg accggcacgc tgcgtgcgga
    15181 taaacagcca cccggagcgc gacgatcccg gccacctcgt atcctggcgc atccaggcgg
    15241 cgctctctca gatctgtgaa gccctggccg gttgcggaac cgcggacaac gcgccgaaca
    15301 agggcgtcga cgcctggttt gaacgtgttc gagactgtca ggagcgcagc gcgcgtgccc
    15361 tggaccgctt cttttcggct caccatgatc tgatcgagcc agcggttgca cgatacatcg
    15421 ccggggcggt tgagcagtcc gatggattgt tcctgggaag cagcatgccc atccgggatg
    15481 cgcagcgcta tgcctccgca tgcgggcagg gacccgtggt tttcgccaac cgcggcgcca
    15541 gcgggattga cggcagcatc gccaccgtgg cgggcgcggc ttttggaagc ggccgcacgc
    15601 tgacggcgat tgtgggcgat ctcgcagcac ttcatgacct gaacagtctc gcgctgctgc
    15661 gtacgataaa gactcccacg gtgctggtga ttatcaacaa taacggcggc ggcatcttcc
    15721 attttttgcc ggtttcgtct caaaccgcgg tctttgatcc ctatttcaca gcgccccacg
    15781 ggctctcttt caaggacgcc gcggcgctct tcggtctgcc ctacgcgaaa ccggactcga
    15841 tggatgatct gaaagaggcg tacaccgctg cgctggaaca agccggcgcc acgctgatcg
    15901 agataaccac cggccgtgcg gagaatgtgg ctctccacaa agtaattgac gaagaggtgt
    15961 tcaaggctct gcaggcctga cgaaccacgg gctgagggtc atggcgcgta cgcttcatta
    16021 ttggatgctg aatcccgccg gagaaaaaac ggtcgtgttt ctgcacggat tcatgggttc
    16081 cagcgaggac tggcgaccga ttgcccaagg attgccgccc aactaccgct gcatagcacc
    16141 ggatctgcct ggtcacgggc gcacgccggt gcgtcgcgat gaggccgcct acacgatggc
    16201 tgcggtgtcg aaggatttga tgcgcctgat ggaccacatc gcgcccggcc cgcttgcact
    16261 tgtgggttat tcgatgggag gccgccttgc cctctacctg gcactggagt atgccgatcg
    16321 cgtgcaggca ctggtgctgg aatcggcctc gccgggcctg cgcagcgaaa cggagcgcgc
    16381 gaaacggcgc gtagaagatg agcggcgtgc ggtcgagctg gagtccggcg actgggaggc
    16441 gttcctgcgg cgatggtacg ccgcgccgct gttcgaatct ctgcacggtt gcggcgatcg
    16501 gtttgaggag ctgctgatgc ggcgccgcaa caacaatccc cgcgagctgg cccgtgtcct
    16561 gcgcggcatg ggcacaggag cgcaatcctc gttgtgggac cggctgtcgg aggcaggcat
    16621 gcctacgctg ctgatcgcgg gagaacgtga cgccaaatac tgcgcaatgg ccgcggcgat
    16681 gggcgcgaag atgccccgcg caaccgcgat ggtggtgtcc ggggcgggcc acaatgtgca
    16741 cttcgaaaaa ccggcggact atactacgct cttgcggggt tttcttgacg gtctgcgcgg
    16801 aacggccgcg caaccgccgc agcatccagg aacgcgggag cagcagtgat ggccagtatc
    16861 gaatggacca gcgccggcgc ctttacggac atccggtacg aaaaggcgga aggcatcgcc
    16921 aaaatcacca tcaaccgtcc ggaagtgcgc aacgcgttcc ggccgctgac cgtcgatgag
    16981 atgtccgcgg cgctatcgga cgcgcgtttt gacccggagg tgggggtcat catcccgacg
//
LOCUS       DAOPBQ010000014        16933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_3450_length_16933_cov_4.076846, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000014 DAOPBQ010000000
VERSION     DAOPBQ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 16933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16933
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3450_length_16933_cov_4.076846"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKW60_01090"
     CDS             complement(<1..512)
                     /locus_tag="PKW60_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98055.1"
                     /translation="MKRIRVAIVGQGRSGRGIHAEYLQRHPGRFTIAAVVDPIKARRD
                     RAVEEFGCDAYVNHKPLLKRRDIDLVVNATPSQLHAPYTLEFLNAGFNVLCEKPLAAR
                     VADVDRLIAAQKKSGTVFAIFQQSRYAPYFQQVRKVIDSGVLGGIVQVNIQFNGFSRR
                     YDWQTLTEFDG"
     gene            718..2178
                     /locus_tag="PKW60_01095"
     CDS             718..2178
                     /locus_tag="PKW60_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPJ98056.1"
                     /translation="MPSAGQDPGLGPIGQAMVSLDFDQAIFLSDHEARKVKTYLKWLK
                     SHTEAGLEVHAVSLSSPMHFGEIYENVSRVLAEHLTKAAPDEERFYHLSPGTPAMTAV
                     WIIAAKTRFPATLLESSIQEGVQVASVPFDISADYLPDLLRKPDEELQRLSQGLPPEA
                     PEFGDIIHRCAAMRHLVARARRVAPRNVPVLILGESGTGKELLARAIHRASTFASGPF
                     VAVNCGAIPAALVEAELFGHEKGAFTGAGTARKGYIEESSGGTLFLDEVGELPRETQV
                     KLLRVLQEFEVTPVGASKPRRVHFRLITATNRDLFRDVSENSFREDLFHRIAVAILQV
                     PPLRERDGDIGLLVDTLLEKVNLEAQGQPGFEHKGLSVSARNLLINHPWPGNIRELMN
                     TLQRAAVWSPGPLIDRSDIEEALLNFLAKTGEGILNRPLANGLCLQDIVAEVSRHYLS
                     RAMEESGGNKTIAARLLGLPSYQTLTNWLKRYGLEA"
     gene            2240..3592
                     /locus_tag="PKW60_01100"
     CDS             2240..3592
                     /locus_tag="PKW60_01100"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HPJ98057.1"
                     /translation="MHDGVRQQAQSGVCTRTGCGVERSCRKEFTMPDQKVKCPNCGTQ
                     FAVEDALTDSIRAQMKSELEAEFAKKQEEATKRLNKAKELEKSIKEREQAIEDTVAER
                     LDEERRKVKADERKRAEREFKHQLEDLQEQIEEGNKKLGEANKREKKFLQRERELQEK
                     EKSIDLEIERRLTDSMKKLQEEAEKRAEEAQQLKLREKDNLINGLQDQIKNLQKRIEQ
                     GSQESQGEALEGQLQDTLAGMFPFDAFEEVKKGARGADIVQTVRDRQGRTCGKILWEA
                     KNAKNFQNSWVSKLKQDQIETGAALAVLMTMALPAQIKQFGQIEDEGVWITDYTSAVG
                     LCAALREQLIRVNREKMMVEHRETAKDILFEYVTGQDFSLRVRIIVDTYIQMQQDLES
                     EKRALQRQWKKRERQIAMVLDSIVGMRGELEGMVGTLTALPGTDSLSLEHLAEDEEPF
                     "
     gene            3624..5459
                     /locus_tag="PKW60_01105"
     CDS             3624..5459
                     /locus_tag="PKW60_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HPJ98058.1"
                     /translation="MLRAYKPATLTAITLENFKAFGKPARVPLAPITLLFGENSAGKS
                     TILQALYLLKQTRELAPPDVPLLLRAENGYVDLGSFEEMVHGHDTHREVSIRFDVEGD
                     PKLAPVREEVSDDLPRRVGMRFRFAKDEGRTGPRLSGFDVFARTPHFLERKGALESVG
                     IHYHKEPLIAYSSAIFDDPHFGSRVGLKITRLPSDPCYWRNALRFLEIELMSESVNIE
                     QERRHELYRKLLESTRLPMPDDVQISQHDEDVKYLLLEHYVLDVGFEPEHTWLTPSEV
                     LSNATPEDLRSAIQEAWQIRKVRETMMRWIETENYGLIKYLEHFIPSPVVYTRELIDS
                     DRYEELCDREGLSLWFDDEDEWFQAAQLFSPIYIEQMTPRLDLESVTYGVAYLVDSAC
                     SVRIIPFEPLRAAPRRFYPTSGLKVEGVGTKGELLGDFLAQRKDVTKRVNTWLRRLGI
                     DYSIAIKPIRTSTSQLFEIRLRDTRQKNAKTVNIADVGSGIAQILPFLAQSLAEEGVL
                     ITIEQPELHIHPRLQAELGSLFAEGIKKGNRFIIETHSEHLILRLQKLIRTGELKPDD
                     VAVAYVARGPEGSTVEQLRLDEKGDFIDDWPGGFFPERLKELEDE"
     gene            5461..6477
                     /locus_tag="PKW60_01110"
     CDS             5461..6477
                     /locus_tag="PKW60_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98059.1"
                     /translation="MYVAVVLHPDCLKKEYFESEGYSTQARLLFKGIQSNGLIIVDSD
                     DRLKRMVGNAIRGLPTQHGQRLQIWWEEFLKKPKRIVEARPERRATLQRIDDELLPGE
                     LALKCPVDAVIVEDAREMNEVALSKYDESEVEERRRSFMDGSGMLDTLAVEHADDLII
                     RAIRYSKWLRFYDKQIGKGGNNLKAFRRGIGHILSLWQKHGYFASDKEGQVTIYTCEA
                     DPILDGEIGRRADEKRQSNCRAYQSVLKHLIDPLRSEFPGWEIEPCVKRQIKNITHPR
                     HLEAQAAIISFERGFDFLEKSEQVEYKRTCLHVDNKVREHLAEYRKLPEAEYLKGCPP
                     ALGD"
     gene            6803..7336
                     /gene="rplJ"
                     /locus_tag="PKW60_01115"
     CDS             6803..7336
                     /gene="rplJ"
                     /locus_tag="PKW60_01115"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPJ98060.1"
                     /translation="MAKQEKIDAVAEIKERIEASQIVVMTKYIGMNAAQATQLRARLR
                     AANVKFKVYKNTLAKRALDELNLSDAAQFLDGPIGWAFSEDPVMPAKVLAEFGKEVPV
                     VQMSGGILDSSIISFAQLKALADLPARDTLLAQVVGTIAAPLRNFVGVLNAVPRNLVN
                     VLDQIRQQKETQGESAA"
     gene            7375..7767
                     /gene="rplL"
                     /locus_tag="PKW60_01120"
     CDS             7375..7767
                     /gene="rplL"
                     /locus_tag="PKW60_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPJ98061.1"
                     /translation="MADKLNAIIEEIAGLSVLELSELIKALEEKFGVTAAAPMMMAGV
                     MPAAGGAEGAEDEGPSSYDAILKEVGSQKIQVIKEVRAITGLGLKEAKELVDGAPKAI
                     KEDCTEEEAKEIKQKIEAVGAVVELKGK"
     gene            complement(7851..8468)
                     /gene="lexA"
                     /locus_tag="PKW60_01125"
     CDS             complement(7851..8468)
                     /gene="lexA"
                     /locus_tag="PKW60_01125"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPJ98062.1"
                     /translation="MAKGLTRRQAEILEYIVCCIRDNGMPPTIDEIGRRFQIASTNGV
                     NDHLVALTRKGYIERTSKARSIRVTGKGAANLYGAGATMVPLVGRIAAGSPILAEENI
                     DGTIPVSPALGLEDGYSLRVQGDSMIEDGILDGDLILVDPNRRPRRGDIVVALVQDEA
                     TVKRFYPQGEMLELRPANAAMQSLLLPARDVQIQGVVVGLQRTFD"
     gene            8752..9717
                     /locus_tag="PKW60_01130"
     CDS             8752..9717
                     /locus_tag="PKW60_01130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPJ98063.1"
                     /translation="MRTLKKFVVAVMVAGVSVCQAELVDQILATVDTEAILQSDLMME
                     ITPRLAELQSTSTDAASFQRAADALVREALEQAIDAKLLLREALRAGIEIDEKRIDEQ
                     LAEFKKMYDSPEQFTKELEAAGETLGDLRVRIRKQSMARAMAVRKQSEYEKQVAVSES
                     DLAQYFEDHKDEFARPERVHVSQVFQPASGDAEERAVVKARMEEILSELKQGGDFAAV
                     AKAYSKGPEAQQGGVIGWIVRGDLVQLLEEAAFALPPEGLSDVLESSGGFHILRVNEH
                     QEAGQATLDDVRKDIEPRLRAEAAMLQYRNWVQELRNKSRVRVFY"
     gene            9754..10551
                     /locus_tag="PKW60_01135"
     CDS             9754..10551
                     /locus_tag="PKW60_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98064.1"
                     /translation="MLARAMKMAFWITYDHLGKFLVAGLLWSLSLLVPAWVAVTALLS
                     GQPGVRAAIGLPAALFAVGVVLPVPSAGLAHLVKVLIDHRDGSLGDFFRGMRLYALKS
                     VLIGLIAVFALASLSTSTWFYADKLRGSAPWLGYALSGIALWGLVFAAPMALLVLPTL
                     VQKKAGIGATLKLSALLVLGHPLFSLGLALQALALTAVCLIIFPLFLFVYGPILAALT
                     GSAYEMLARKYAPTDAGGPASQPPYPTDDEDDYLNRGFRDFLFPWKG"
     gene            10739..11578
                     /locus_tag="PKW60_01140"
     CDS             10739..11578
                     /locus_tag="PKW60_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98065.1"
                     /translation="MAHQHIRTLIASGVLATGMLMWAVSGLAEPVDADAPTEFGATAA
                     EEYSKWFFIFGVANVHPRLEASEAQIDRQINGLLGPLFPRWEEPRTFVDWREELRIWD
                     LHFGFGRELTPKWVWLSTFGGSLGTVRNEHRYYPLAMPSMFHIDFSRKFWFASTGMLY
                     YPWGQSLYEPAEGALERFRRSMRGTRPFIGAVGAYLDLHAEAVVQIDLPLTKFDPRIV
                     QKQRARSFYGSPRIGVDVPLTEQGDLVLAAGYLFFCEDSADLDNWSFYVMYKHKLKAR
                     KRD"
     gene            11851..13779
                     /locus_tag="PKW60_01145"
     CDS             11851..13779
                     /locus_tag="PKW60_01145"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPJ98066.1"
                     /translation="MSMLMRKYKKEILWVTIILIVGPFVVWGGYRSAHHADPDLESAL
                     APVAVIDGSPISAAEYREALNQEVQQRRRFGQEAEFADLLADGSAQRVMEAMVSRRLL
                     EVEAEKSPYSFDKDYLVEQLKDQFKDDQGRFDGEQFNQWVQMIDKRSGQSWNQIYAGV
                     ADQMRHGLVIAEIAASARVSEKELKSQFEDAHTKLDLKYAAIAPSIEPTAEEIQAQYD
                     ADPSVYDIPGKRKADFVALSLRPPVPAIAAELIQKARAGEDFAELAKAHSEGADAAEG
                     GDMGWVVKTPQTPAFQEPLFALKPGEVSGVVEGPAGQYFIYKAEEERESAVLPDVRDI
                     KARRIVLSPRLSPDENTALTEKAEALLAAAKESGDLAAAAAEAGCQVQTTDLFGPDSP
                     AIASIPQEDVFRFRTGVSELAQGVFSNVISGQANLYVAKVVELTPAEPQPFEAVKDRV
                     RDDAIETARRSPEYIEKCRALAEEIKGNVTVLAEIPEKHPDLQTEVVELDGFSQQDYM
                     SLGGLAVNPRQLYALAKGKAPGDVFGPLQDFMGQTYLVQLVAKHPPTDADWEEKWPQE
                     HDLLRDRALAMAQNQRLSDYLQYLRDTHPWGLVEKTFQGIFQTYDEETEAPAGKSAAP
                     APGGDDAPAADSGGETQP"
     gene            13839..14990
                     /locus_tag="PKW60_01150"
     CDS             13839..14990
                     /locus_tag="PKW60_01150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPJ98067.1"
                     /translation="MRENRRMNVLHLFSNSKWTGPAEPALNLCVALRRQGVEVAFACA
                     PGSGASINKVAETARDRGIEPVLAFRLNKHHNPLTNALDRVALRRYLQAHPFDLLHCH
                     LDNDHRIAAPVARSLGIPLIRSNYYGGGLPAGRAQTRLLATATHVIEPSHMAAAHDTR
                     AHGLEPSRVTVVPGAVDTERFHPGRDVPDGRRWLKIPPDAFVVGIVARMQTHRHYEDF
                     FEAIRKLVDRHSHARVIVVGRGTRREEVGLEPVRRLGLENYVHFPGYLDGENYVGMLK
                     AFDVKVFLVPGSDGTCRAVRESMAMRKPAIVADRGMLREIVEHEKNGLVCEGSAESLF
                     HALARLAEDRRLLHQLGQAARDTAATHYSLESQATAVRAIYERVLARGM"
     gene            15216..16337
                     /locus_tag="PKW60_01155"
     CDS             15216..16337
                     /locus_tag="PKW60_01155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPJ98068.1"
                     /translation="MTRICYIIPTLSAGGAERQLLELVRELVRDHEVTVICTRRAGVL
                     GGDARRLGAYVRELHLRGSGDFRARSRLRRTFRSHRPDVLHTFLSGLDYPASCAARET
                     GVPVIVSSRRQLAAWKRGRFIRQQRRANELVDCIVANSQAVAQFAIEQEQADPALFRV
                     IPEGIAVESMKSHSDPEQVRARYGLPRGKYVVGMVANFTPPKDYALFVEIANTLVKRR
                     DDVHFLAVGAGPLVRPVWRQIRQAGLEAHFTRTSTLSELADIYSAMDVSVLCPRTAGC
                     PRAVLEAMAARRPVVAARVGGIPEILQDGETGRLIEGRDPALFAEAIEPLLNDEAEAR
                     RLGEAAHAYVREHLSADRMADACRQLYAELLIRAARTGR"
     gene            complement(16419..>16933)
                     /locus_tag="PKW60_01160"
     CDS             complement(16419..>16933)
                     /locus_tag="PKW60_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98069.1"
                     /translation="KWRHWLVAGVLIVAASSSLSNGVQVFWLRSAYPEVLQWLRAQGE
                     TTIASSQPGPVSWYLACEGMEQPGEASPHGFVTDWQELREGRYPEESSRIVAGAEPVI
                     VFEYRFDRIFLDLEAIPRKPSSILRTLVTTRDLDLDRARRVPVYRLSDTVPAQKPPIS
                     AIRPVPVLPP"
BASE COUNT         3551 a         4718 c         5385 g         3279 t
ORIGIN      
        1 ccgtcgaact cggtgagcgt ctgccagtcg tagcggcggc tgaacccgtt gaactggatg
       61 ttgacctgca caatgccgcc caggacaccc gagtcgatca ccttgcgcac ctgctgaaaa
      121 tacggggcgt aacgcgactg ctgaaagatc gcgaacacgg tcccgctctt cttctgcgcg
      181 gcgatgagcc gatccacgtc cgccactcgg gctgcaagag gcttttcgca gagcacattg
      241 aatcccgcgt tcagaaattc aagcgtgtac ggcgcatgca gctggctggg cgtcgcattg
      301 acgaccaggt cgatgtcgcg gcgcttcaga agaggcttgt ggttcacata ggcatcgcat
      361 ccgaactcct caacggcccg atcgcggcgc gccttgatag ggtcgactac ggcggcaatc
      421 gtaaatctgc cggggtggcg ttgcaggtat tccgcgtgaa tgccgcggcc gctgcggccc
      481 tgaccgacga tcgcaacccg gatgcgtttc atgacagctt ccttcccaca aatggcgcct
      541 gtgatgatgt ggcatgtaat agccgttttg cgcgcgtgag gtcaaatcgg cggaaaggaa
      601 gaggaggcgc tttaagaagc atccttgtgt ttggacaaaa tttcttctat tctgaactct
      661 atgttcaaaa cgctctttgc ctggatagga catgcggatc tgaaggcagc aggggcgatg
      721 cccagcgctg ggcaggatcc cggcctcggt ccgatcggcc aagccatggt ctcgctggat
      781 ttcgaccagg cgattttctt gtccgaccac gaggcgagga aggtcaaaac ctacctgaaa
      841 tggctcaagt cgcatacaga ggctggcctt gaagtccatg cggtatctct ttccagcccg
      901 atgcattttg gcgaaattta tgagaatgtc agccgggttt tggccgagca tcttaccaaa
      961 gccgccccgg atgaagaacg gttctatcat ttaagccccg gcacgccggc gatgactgcg
     1021 gtctggatca tcgcggcgaa gacccgtttc cccgccacgc tcctcgaatc ctcgattcaa
     1081 gaaggcgttc aggtggcatc ggtgccgttt gacatttcgg cggattatct ccccgatctc
     1141 ctccgaaagc ccgacgagga gctccagcgc ctttctcaag ggctcccgcc cgaagcaccg
     1201 gagttcggcg acatcatcca ccgatgcgcc gccatgaggc atcttgttgc acgcgcacgg
     1261 cgcgtcgctc cgcgaaacgt tcccgtgctg atcctgggcg aatcgggtac gggcaaggaa
     1321 ctcctggctc gggcgattca tcgcgcgagc acgtttgcct ccggaccgtt tgtggcggtc
     1381 aattgcggcg ccattcccgc ggcgcttgtc gaggcggagc tctttggtca cgagaaaggt
     1441 gccttcactg gggccggaac ggcgagaaaa ggctatatag aagaaagcag cggcggcaca
     1501 cttttcctgg acgaggtcgg ggaattgcca cgggaaacgc aggtgaagct gctccgagtg
     1561 ctgcaggagt tcgaggtgac tcccgtgggt gcaagcaaac cccgccgggt acacttccgt
     1621 ctgattaccg caacaaaccg cgacctgttc cgcgacgtct ctgaaaacag cttccgggaa
     1681 gacctgttcc accggatcgc ggtggccatt ctgcaagtgc cgccattgcg cgaacgggac
     1741 ggcgatatcg ggctcctggt tgacaccctc ctcgagaagg tcaacctgga ggcccagggc
     1801 cagcccggct tcgaacacaa aggactttct gtatccgcaa gaaatcttct aataaaccat
     1861 ccctggccgg gcaatatccg cgagctgatg aacaccctcc agcgggccgc cgtgtggtcg
     1921 cctggcccct tgattgaccg ctcagatatc gaggaggcgc tgctcaattt tctcgcaaag
     1981 accggcgaag gcattctgaa ccggcctctt gcaaatggcc tgtgtttgca ggatattgta
     2041 gcagaggtgt cccggcacta tttgtcgcgg gccatggagg aaagtggggg caacaagacc
     2101 atagccgccc gcctgcttgg actacccagc tatcagacgc tcaccaactg gctcaagagg
     2161 tacggcctgg aagcctaggc ctttttgaga tactacaggc tgcccactat gtcgattggc
     2221 atgaggcttg cggtaagtaa tgcatgacgg cgtgaggcag caagcgcaat caggcgtttg
     2281 cacacgaacc ggctgcggtg tcgagcgttc atgcagaaag gagttcacca tgcccgatca
     2341 gaaggttaaa tgcccgaatt gtggaactca gttcgcggtt gaagatgccc tcaccgactc
     2401 tattcgcgcc caaatgaaaa gcgagctcga ggcggagttt gccaaaaagc aggaagaggc
     2461 aaccaagcgg ctgaataagg ccaaagaact tgagaagtcc attaaggagc gagaacaggc
     2521 cattgaggac accgtggccg agcgccttga cgaagaacgc aggaaagtca aggcggatga
     2581 gcgcaagcgt gcggagcgtg aattcaagca tcagttggaa gaccttcagg aacaaatcga
     2641 ggaaggcaat aaaaagctgg gagaggcgaa taagcgtgaa aagaagtttc tgcaacgcga
     2701 gcgggaactc caggaaaaag agaagtctat tgatttggag atcgagcggc gcttgaccga
     2761 ttccatgaag aagcttcaag aagaagcaga gaagagggcc gaagaagcgc agcagctgaa
     2821 gctccgtgag aaggacaact tgattaacgg gcttcaggat caaatcaaaa atctccagaa
     2881 acgcatcgaa caaggctcgc aggagagtca gggagaggcg ctggaagggc aattgcagga
     2941 tacccttgcc gggatgttcc cttttgatgc ttttgaggaa gtgaagaagg gcgcccgggg
     3001 cgctgatatc gtgcagacag tacgagaccg gcaaggccgg acctgcggca aaatcctctg
     3061 ggaggcaaag aacgccaaaa acttccagaa ttcgtgggtg agcaagttga agcaggacca
     3121 gatagagaca ggcgcggccc tggccgtgct catgacaatg gcgctcccgg cgcaaatcaa
     3181 acagtttggg cagatcgagg acgagggtgt ctggataaca gactacacgt ccgcggtggg
     3241 gctgtgcgcc gcactccgtg agcagttgat ccgcgtcaat cgcgaaaaga tgatggtgga
     3301 gcatcgcgaa accgccaaag acatcctgtt cgagtatgtc acggggcaag acttcagctt
     3361 gcgtgttcgc atcattgttg atacgtacat ccagatgcag caggacctgg aaagcgaaaa
     3421 gcgggccctg cagcggcaat ggaaaaaaag agagaggcag atcgccatgg tcctcgacag
     3481 catcgtgggg atgagaggcg agctggaagg tatggtcggc acacttaccg ctctgccggg
     3541 tacggacagc ctcagtctgg agcacctggc cgaggatgag gaaccgttct aatgcgcaat
     3601 gggcgtatac gcggagaaat caaatgctga gagcctataa accagccact ctaactgcga
     3661 taacgctgga gaacttcaaa gcattcggga aaccggcgcg agtgccgctg gcgccgatca
     3721 cgctgctgtt cggtgagaac agcgcgggca aaagcaccat cctccaggcg ctctacctcc
     3781 tcaaacaaac ccgcgaattg gctcctcccg acgtccccct gctcctgcgc gccgaaaacg
     3841 gctacgttga tctgggaagt tttgaagaga tggttcatgg ccatgacact catagggagg
     3901 tcagcatacg attcgatgtt gaaggcgacc ccaagctggc tccagtgaga gaagaagtca
     3961 gcgatgacct tccgcgccga gtggggatgc gttttcgttt tgctaaggat gaaggacgca
     4021 ccgggcctcg tctttctggg tttgatgtct ttgccaggac cccccatttt ttagagagaa
     4081 aaggggccct ggaaagcgtt ggcattcact accataagga acccttgatt gcttattctt
     4141 ccgcgatttt tgatgaccca cactttggtt ctagagtcgg tttgaagatc acacgattgc
     4201 cctcagatcc atgctattgg cgtaatgcac tgcggtttct tgagatagaa ttgatgtcgg
     4261 aatctgtgaa catagagcaa gaacgcagac atgaactgta tcgaaagttg ctcgaatcga
     4321 cgcgtttgcc gatgcctgat gatgtccaga tctctcaaca cgacgaagac gtgaagtacc
     4381 ttctactgga gcattatgtc ttggacgtag gttttgaacc tgaacataca tggctaaccc
     4441 cttctgaagt actgagcaat gccacgccgg aggacctgcg cagtgcgatc caggaggcat
     4501 ggcagatcag aaaagttcgc gaaacgatga tgagatggat tgagacggag aattatggtc
     4561 tcatcaaata tctcgagcac ttcataccaa gcccagtggt atacacgaga gaactaatcg
     4621 attctgaccg ttacgaagaa ctctgtgatc gggagggtct gtctctttgg tttgatgatg
     4681 aggacgaatg gtttcaagca gcgcaactat tcagccctat atatatagaa cagatgacgc
     4741 ctcgcctcga cttggagagc gtcacttatg gggtcgccta tttggtagat tcagcttgca
     4801 gcgtccgcat tattcccttc gagccgcttc gcgctgcccc caggcggttc tacccaacca
     4861 gcggattgaa ggtagaaggt gtgggcacaa agggtgagct gcttggggat ttcctggcac
     4921 aaagaaaaga tgtcacaaaa cgagtcaaca catggctgcg tcgactgggc atagattatt
     4981 cgatcgcgat caaacccatt agaacgtcta catcgcagtt gtttgagatt cgccttcgag
     5041 acacacggca aaaaaacgcc aaaacagtca atatcgcgga tgtgggttcg ggtatcgcac
     5101 aaattcttcc gttcttagct caatctctcg cggaagaagg agtactcatc accatcgaac
     5161 agcctgagct tcatatccat ccccgtcttc aagcggaact gggcagcctg ttcgcagagg
     5221 gcatcaagaa gggcaaccgg ttcatcatcg agacgcacag cgagcatctt atcctccggc
     5281 tccagaaact catccggacc ggggagctga aacctgatga cgtggccgtg gcctatgtgg
     5341 cccgtggccc tgaaggctca accgtagagc agctccgcct cgacgagaaa ggcgatttca
     5401 tagacgactg gccgggaggc tttttccctg agcggttgaa ggaactggag gacgaatagc
     5461 atgtatgtgg cggtggtcct tcatcccgat tgcctcaaga aagagtattt cgagtcagag
     5521 ggctatagta cgcaggcgag attgctattc aaagggattc agagcaacgg attgatcatt
     5581 gttgattcgg acgaccggct gaagcgcatg gttggaaatg ctatccgcgg cttgcccacc
     5641 caacacgggc aacgcctgca gatttggtgg gaggaatttc tgaaaaagcc taagcgaatc
     5701 gttgaggcac gccccgagag gcgtgccacg ttgcaacgga ttgatgatga actcctgcca
     5761 ggcgagcttg ccctaaaatg tccagtggat gcagttatcg tggaagatgc ccgggaaatg
     5821 aatgaagtcg ctttgagtaa gtatgatgag agcgaggtcg aggaaaggcg gcggagtttt
     5881 atggacggtt cgggcatgtt ggacactctc gctgttgagc atgcggatga cttgatcatt
     5941 cgcgctattc gctattcaaa atggctgcgc ttctacgaca aacagatcgg gaagggcggt
     6001 aataacctca aagccttcag gcgcggcatt ggacacatcc tttccctttg gcaaaagcac
     6061 ggctattttg cttccgacaa agagggccag gtgaccattt acacgtgcga agcggaccca
     6121 atactggatg gagaaatagg tagaagagcc gacgagaaga ggcaaagtaa ttgccgggcc
     6181 taccaaagcg tgttgaagca tcttattgac ccgctgcgca gcgagttccc tggatgggag
     6241 atagagcctt gcgtcaagcg acagataaaa aatatcactc atccgcggca cctggaggca
     6301 caggctgcaa taatctcctt tgaacgtggc ttcgatttcc tggaaaaaag tgagcaagtt
     6361 gaatataagc ggacttgctt acacgtcgac aacaaagtgc gcgagcatct tgcggagtat
     6421 cggaagcttc cggaggcgga atatttgaag ggatgccctc cggccctcgg agactagcga
     6481 cattgacagg agccaccata ggcgccgtta gactgcacgg cgtcgttagt acggcggcga
     6541 taatcgcagg gggtagagat ccctcccatg ggcggatggc gccgcgtttg acttggctgt
     6601 tgggcgtttg ctagactttg cggctgaaac gatagatcgc ctcgtgtagg aacgcatcga
     6661 agacagccgg cggccgtgat ggccttaaaa ttcgcctgcc gagaccgcgg agcacgacgg
     6721 aatcccgagt gaattctgtc ccgcaccgtc ttcgaccgtg cgggattttt ttgttgaggc
     6781 gagggaaggg aggtgacagc ttttggctaa acaggaaaaa attgatgcgg tggccgagat
     6841 caaggagcgt attgaagcca gtcagatcgt cgtgatgacg aagtacattg gcatgaacgc
     6901 ggctcaggcc acccagcttc gcgcgagact gcgcgccgcc aatgtgaagt tcaaggtcta
     6961 caagaacacg ctggcgaaac gcgcgctgga cgaactgaac ctgtcggatg ccgcccagtt
     7021 tctggacggc ccgattggat gggcgttctc ggaagacccc gtcatgccgg ccaaggttct
     7081 cgcggagttt ggcaaagaag tgcccgtggt gcagatgagc gggggcattc ttgacagcag
     7141 catcatttcg tttgcgcagc tgaaggccct tgcggacctg ccggcacgcg acactctgct
     7201 tgcgcaggtg gtcggaacga ttgccgcgcc gttgcgcaat ttcgtgggcg tgctcaacgc
     7261 cgtaccccgc aatctggtca atgtgcttga ccagattcgc cagcagaaag aaacccaggg
     7321 agaaagcgcc gcttgatttg cccgcggcgg gaatgtgaca ggagttacga cgagatggca
     7381 gacaaactga atgcgattat cgaagaaatc gcggggctgt cggtgctcga gctgagcgaa
     7441 ctgatcaagg ccctcgaaga gaaatttggt gtaaccgccg ccgcgcccat gatgatggcg
     7501 ggggtcatgc cggccgcggg tggcgccgaa ggcgccgagg atgaaggccc cagctcgtat
     7561 gatgcgatcc tgaaggaagt cggctcgcag aagatccagg tgatcaagga agtgcgcgcg
     7621 atcacgggtc tcggtctgaa agaggccaag gaactcgttg acggcgctcc gaaggcgatc
     7681 aaagaagact gcaccgaaga agaagccaaa gagatcaagc agaagatcga ggccgtcggc
     7741 gcggttgttg agcttaaagg caagtaatcc cggttttcag acaggattcc atcgcggcag
     7801 gctgcttcgg cggcctgccg cgtgccgttt cagcgccagg tttgcgaggt ttaatcgaag
     7861 gtgcgctgca ggccaaccac cacgccctgg atttgcacgt cgcgcgcggg aagcagcagc
     7921 gactgcatgg ccgcgttggc gggccgcaat tccagcatct cgccctgcgg ataaaaccgt
     7981 ttgacggtcg cctcgtcctg caccagtgca acgacgatgt cgccccggcg cggacggcga
     8041 ttcgggtcga cgagaatgag gtcgccgtcg aggatgccgt cttcaatcat gctgtcgccc
     8101 tgcacgcgga gcgaatagcc atcctccagg cccagcgcgg gggagacggg gatggtgccg
     8161 tcaatgttct cttctgccag gatgggggag cctgcggcaa tgcggccgac caggggcacc
     8221 atggttgccc cggcgccgta caggttggct gcgcctttcc cggtcacccg gatggaacgc
     8281 gctttggagg tccgctcgat gtagcctttg cgcgtgaggg ccacgagatg gtcgtttacg
     8341 ccgttggtcg aggcgatctg gaagcggcgc ccgatctcgt caatggtggg gggcattccg
     8401 ttgtcacgga tgcagcagac gatgtactcc aggatttccg cctggcggcg cgttaaccct
     8461 tttgccatgt agcctccttg cactatttca ggcagactct gaattctttt tcagcatttt
     8521 atccaatcac tgaagatctg tcaagcggtt gcgactcctg gcgcgcgggc gtgatgggag
     8581 gggaggcaga attcacacgc tccgatgccg cgtgctccgg ttgcgtgggc tggagctgta
     8641 tgctattctg cgtcatcatg acggcggggg attcccgcct gggcgtaact gttcagcagg
     8701 gaagcgtttc catcgctttt ccagcccgaa aaccgagagg atgggccaac cgtgcgtacc
     8761 ctgaagaaat tcgttgttgc ggttatggtg gcgggtgtgt cggtctgcca ggcggaactg
     8821 gtggaccaga ttctcgcgac ggtcgacacg gaagcgatcc tccagagcga cctcatgatg
     8881 gagattacgc cgcgtctggc ggagctgcag tcgacctcga cggatgcggc gtcgtttcaa
     8941 cgggccgcgg acgcgctggt gcgggaggcg ctcgagcagg cgattgatgc gaagctgctg
     9001 ttgcgggaag cgcttcgcgc gggcatcgag atcgatgaaa aacggatcga cgagcagctt
     9061 gcggaattca agaagatgta cgattcgccg gaacagttta cgaaagaact ggaggcagcc
     9121 ggcgagaccc tgggcgatct ccgggtgcgt attcgcaaac agagcatggc ccgggcgatg
     9181 gcggttcgca agcagagcga atacgaaaag caggtcgcgg tgtcggaatc ggaccttgca
     9241 cagtattttg aagatcataa agacgaattc gcacggccgg agcgggtgca tgtaagccag
     9301 gttttccagc ctgccagcgg tgacgcagag gaacgggccg tggtcaaggc gcggatggag
     9361 gaaatcctga gtgaattgaa gcagggcggg gattttgcgg cggttgcgaa ggcgtattcg
     9421 aaagggccgg aagcgcagca gggcggcgtc atcggctgga tcgtacgtgg cgatctcgtg
     9481 cagttgctgg aagaggccgc gtttgccctg ccgccggaag gtctcagcga tgtgctcgaa
     9541 agctccggcg gattccacat cctccgggtg aacgagcacc aggaagcggg ccaggccacg
     9601 ctcgacgatg tgcgcaaaga catcgagccc cggctgcgcg cagaagccgc tatgctgcag
     9661 taccgaaact gggtccagga actgcgcaac aagagccgcg tgcgcgtgtt ctattgatgc
     9721 gccggtacgg ccagccggca tccggatagc ctcatgctcg cgcgtgcgat gaaaatggcg
     9781 ttctggatca cctacgacca tctgggcaaa ttcctggttg cgggtttgct gtggtctctg
     9841 tcgctgctcg tgccggcgtg ggtggcggtc acggccctgc tgagcgggca gccgggggtt
     9901 cgcgccgcca tcgggcttcc cgcggcactg ttcgcggttg gcgtcgtgct gccggttccg
     9961 agcgcggggt tggcgcacct ggtcaaagtc ctgatcgacc atcgcgatgg gagtctgggc
    10021 gatttttttc gcggtatgcg cctgtatgcc ctgaagagcg tgctgatcgg cctgatcgcg
    10081 gtgttcgcgt tggccagcct gagcaccagc acgtggtttt atgcggacaa attgcgcggc
    10141 agtgctccct ggctgggtta tgccctcagc ggcatcgcgc tgtgggggct ggtatttgcc
    10201 gcgcccatgg ccttgcttgt gcttcccacc ctggtgcaga aaaaggctgg aattggcgct
    10261 accctcaagc tcagcgcttt gctggtcctc gggcatccgc tgttttcgct gggattggca
    10321 ctgcaggcgc tggcgttgac ggccgtctgc ctgatcatct tccctctttt tctgtttgtg
    10381 tatgggccga tcctggccgc gctgaccggc agcgcctatg agatgcttgc ccggaaatac
    10441 gcccccactg acgcgggcgg cccggcgtcc cagccgccat atcccaccga cgacgaagac
    10501 gactacctga accgcggatt tcgcgatttt ctgtttccgt ggaaaggata ggcgcggccc
    10561 ttgtgaaggg cgcctgtccg cagcgcagcg ccatccgccc cagggcttct gctcacgggc
    10621 tgatttcggc gcggttgatc tgctcccagc catgcgggta ccatggcgag cggggggaga
    10681 ttaccatctc cggcaactgg aatcacatcc cgtctctgaa tgaaaaaggg ggttctgcat
    10741 ggcacaccag catattcgca ctttgattgc atctggagtc ctggcgacag gcatgctcat
    10801 gtgggcggtt tccggactgg cggagccggt tgacgcagac gcacccacgg agttcggagc
    10861 gaccgcagcg gaagagtact ccaaatggtt tttcattttc ggcgtcgcca atgtgcatcc
    10921 ccggctggag gcgtcggaag cccagatcga ccggcaaatt aacggacttc tcggaccctt
    10981 gtttccccgg tgggaagaac cgcggacctt tgtggattgg cgggaagaac tccgtatctg
    11041 ggatctccat tttgggtttg gccgggagtt gaccccgaaa tgggtctggc tgtcgacctt
    11101 tggcggttcg ctgggcaccg tgcgtaacga acaccgctat tatccgctgg ctatgccatc
    11161 catgttccat atcgattttt cgcgaaaatt ctggtttgcc tccacgggga tgttgtatta
    11221 cccgtggggc cagtcgctgt atgagccggc cgagggcgcg ctggagcgtt tccggcggtc
    11281 gatgcgcgga acgcggccct tcattggagc cgttggcgcc tatctggacc tgcatgccga
    11341 ggcggtggtt caaattgacc tgccattgac gaagtttgat ccgcgtatcg tgcagaaaca
    11401 gcgcgcccgt tcgttttatg gcagcccgcg tatcggggtg gatgtgccgc tgactgaaca
    11461 gggcgacctt gtcctggcgg cgggatacct gtttttctgc gaagacagcg cggacctgga
    11521 caactggtcc ttctacgtga tgtacaagca taagctgaag gcgcggaagc gggactgagc
    11581 caggagcggg ggcagtccag cggaacagga aaagaggcca ggccagcttg cctgcgcccc
    11641 cctggacaac gcgcgcgtcc ccaccaccaa gcgcctggcc gcgcgttgca gaaaggcgga
    11701 tatttcgagt ttgcgtttct cgttccacgg cggctggttt gcgaacaaac ggcaccgtat
    11761 gctacccttc gcgcgtttgc aagaccattt attcccaatt gaatgcgcgc ccgcatggac
    11821 ccgggtgcca ttccggaggt tgtcggcatc atgagcatgc tgatgcgtaa gtacaagaag
    11881 gaaattctgt gggtgacgat tatattgatc gtgggcccat tcgtagtctg gggggggtat
    11941 cgcagcgcac accatgcgga tcccgatctg gagagcgcat tggcgccggt ggcggtgatc
    12001 gatggttcgc ccatttcggc ggcagagtat cgcgaggcgc tcaaccagga ggttcagcag
    12061 cgcagacgct ttgggcagga agcagaattc gccgacctgc tggccgacgg ctccgcacaa
    12121 cgtgtgatgg aagcgatggt gtcccggcgg ctgcttgagg tggaagcgga aaaatctccg
    12181 tacagcttcg acaaagacta cctcgtcgag cagcttaaag accagttcaa ggacgatcag
    12241 gggagattcg acggcgaaca gttcaaccag tgggtgcaaa tgatcgataa gcgttcgggc
    12301 cagtcgtgga accagatcta cgccggcgtt gcggaccaga tgcgccatgg gctcgtaatc
    12361 gcggagatcg cggcttcggc gcgggtgtcg gaaaaagaac tcaagagcca gttcgaggac
    12421 gcccacacga aactcgatct caaatacgcc gccatcgcgc cttccatcga acccaccgca
    12481 gaggagattc aggcccaata cgacgccgat ccctcggtgt atgacattcc cggaaagcgc
    12541 aaggcagatt tcgtggcgct gtcgctgcgg ccgccggtgc cggccatcgc ggccgagctc
    12601 atccagaagg cacgggccgg cgaggatttt gctgaactgg cgaaggcgca ttccgagggc
    12661 gccgatgcgg cggaaggcgg cgacatgggc tgggtggtga agaccccgca gacccccgca
    12721 tttcaggagc cgctgttcgc cctgaagccg ggcgaggtga gcggcgtggt ggaaggcccc
    12781 gccggccagt atttcatcta caaggcggaa gaagagcgtg aaagcgcagt gttgccggac
    12841 gttcgcgaca tcaaagcgcg ccgtatcgtg ctcagcccgc ggctgagccc ggacgaaaac
    12901 acggcgctga cggaaaaagc cgaagcgctt ctggccgccg ccaaagaatc gggcgacctc
    12961 gcggccgcgg ctgctgaggc gggctgccag gtgcagacca cggacctctt cgggcccgac
    13021 agccctgcga tcgcgtcgat tcctcaggaa gatgtgtttc ggttccggac gggggtgtct
    13081 gaactggcgc agggcgtgtt ttcgaacgtc atctccggcc aggccaacct gtatgtggcg
    13141 aaagtggtgg aattgactcc ggccgagccg cagcccttcg aggcggtgaa agaccgggtg
    13201 cgggacgatg ccatcgagac cgcgcgccgc tcgcctgagt acatcgaaaa atgccgcgcg
    13261 cttgcggaag aaatcaaggg caacgtgacc gtgctcgcgg agataccgga gaagcatccc
    13321 gatctccaga ccgaagtagt ggagctggac ggattcagcc agcaggacta tatgtcgctg
    13381 ggcgggttgg cggtcaatcc ccgccagctc tatgccctgg ccaaaggcaa ggcgccgggc
    13441 gacgttttcg gtcctctcca ggatttcatg gggcagacgt atcttgtgca actggtggcc
    13501 aaacatccgc ccacggatgc ggactgggag gagaagtggc cccaggagca tgatctgttg
    13561 cgggatcgcg ctttggccat ggcgcagaat cagcggctct cggattacct gcaatatctg
    13621 cgggataccc acccgtgggg gctcgtggag aagacgttcc agggcatctt ccaaacgtac
    13681 gacgaggaaa cggaagcgcc ggcgggcaaa agcgccgcgc ccgcgcccgg tggcgacgac
    13741 gccccggccg cagactccgg cggcgaaacg caaccgtagc aggccgccac gagtcgcgac
    13801 accgaagaga ggaggtctgg cgcgcgggat gcatttccgt gcgtgaaaac cgccgcatga
    13861 acgtccttca cctcttcagc aactccaaat ggaccggtcc cgcagagcct gcgttgaatc
    13921 tctgcgttgc actgcggcgc cagggcgtag aggttgcgtt cgcgtgcgct cccggttccg
    13981 gtgcgtccat caacaaagtg gcggaaacag cgcgggaccg cggcatcgag cctgttctcg
    14041 catttcgact gaataaacat cacaatccat taaccaacgc gctggatcgg gttgccttgc
    14101 ggcgctacct gcaggctcac cccttcgatc tgcttcactg ccatctcgac aacgaccacc
    14161 ggatcgccgc cccggtagcc cggtcgctgg gcattccgct gattcgttcc aactactacg
    14221 gcggcggctt acccgcgggc cgtgcgcaga cccgcctgct ggccaccgca acgcacgtga
    14281 tcgaaccgtc acacatggcc gcggcgcacg atacgcgcgc gcacggcctt gaaccgtcgc
    14341 gcgtcacggt ggtgcccggc gcggtggaca ccgaacgctt ccatccggga cgtgatgtgc
    14401 cggatgggcg gcgctggctc aagatccctc ccgatgcgtt tgtggtgggg attgtcgcgc
    14461 ggatgcaaac ccaccgccat tacgaggatt tctttgaagc gattcggaaa ctcgtcgatc
    14521 gacattctca cgcgcgcgtg atcgttgtgg gccgcggcac ccggcgtgaa gaggtggggc
    14581 tggaacccgt gcggcggctg ggcctggaaa attacgtgca tttccccggg tatctcgacg
    14641 gcgaaaacta tgtgggcatg ctcaaagcct tcgacgtgaa agtcttcctg gtgcccggaa
    14701 gcgacggcac ctgccgggcc gtgcgtgaaa gcatggccat gcgaaaaccg gcgatcgtgg
    14761 ccgatcgcgg catgcttcgg gaaattgtgg aacacgagaa gaacgggctg gtctgcgagg
    14821 gttccgcgga atcgttgttc cacgcgctgg cccgcctggc ggaagaccgc cgcctgcttc
    14881 accagttggg ccaggccgcg cgcgacacgg cggccaccca ctattcactg gaaagccagg
    14941 caacggctgt ccgcgccatt tacgagcggg tgctcgcccg cgggatgtga cgatctcgca
    15001 atgcggagca tgcttgatcc gagatacggg cagagtcatg ccggtggcgc gcacgcgcct
    15061 tcgtcatttg acgccggctg cggtatcatc ttcgcgtggc tctacgttcc ggcgtatggc
    15121 tgcccgcacg gggaatacgg atgcgccagg gttggatcgc ggcaaaccct gcgtcaggct
    15181 gcgctgcgta tgctggagct gcgggagtcg tcattgtgac ccgaatctgc tacatcattc
    15241 caacgctgag cgccggtggc gctgaacggc agcttctgga actcgtccgg gagctggttc
    15301 gcgaccacga agtgacggtc atttgcacgc ggcgggcggg agtgctgggc ggggatgccc
    15361 gccggctggg cgcctacgtg cgcgagctgc atttacgcgg cagcggcgac ttccgcgcgc
    15421 ggtcccggct gcggcgcacc tttcgctcgc accggcctga tgtcctgcac acctttttgt
    15481 ccggactgga ttatccggcc agctgcgcgg cccgggagac cggggttccc gtgatagtct
    15541 cgagccgccg gcaactggcc gcgtggaaga gaggccgctt catccggcag caacgcaggg
    15601 cgaacgagct ggtcgactgc attgtcgcca acagccaggc cgttgcgcag ttcgccattg
    15661 agcaggagca agccgacccc gccctgtttc gcgtgattcc ggaagggatc gcggtggaat
    15721 cgatgaaaag ccacagcgat ccggagcagg tacgcgcgcg gtatggtctg ccccgcggga
    15781 aatatgtcgt ggggatggtg gccaatttca ccccgcccaa ggattatgcc ctgtttgtcg
    15841 agatcgcgaa caccctggtg aaacgccggg acgacgttca ttttctcgcg gtgggcgccg
    15901 gaccgctggt gaggccggtg tggcggcaga tcagacaggc cggcctggag gcccatttca
    15961 cccgcacctc cactttgagc gaactggcgg acatctacag cgccatggac gtctcggtgc
    16021 tgtgcccccg gaccgcaggc tgcccccgtg ccgtactcga ggccatggcg gcccggcggc
    16081 cagtcgtcgc ggcccgcgtg ggcggcatcc ccgaaatcct gcaggacggc gagaccggca
    16141 ggctgatcga aggccgggat ccggccttgt ttgcggaggc catcgaaccg ttgttgaacg
    16201 acgaggccga ggcgcgccgc ctgggtgagg ctgcccacgc gtatgtgcgc gaacacctga
    16261 gtgcagaccg gatggccgac gcctgccggc aactgtatgc ggaactgctc atccgcgcgg
    16321 cgcgcaccgg gagatgaccc cggcagcagc gtctcaggcc gggccacgcg gtgcggcggc
    16381 agaaggaaca tccggtgtcc gctgtttcag gccgttgatc acggcggaag tacgggaacc
    16441 gggcggatcg cactgatcgg gggtttttgt gcgggcacgg tgtcgctcaa ccggtaaacg
    16501 ggcacccgtc gcgcgcggtc gagatccagg tcgcgcgttg taaccagagt gcgcaaaatt
    16561 gatgacggtt tgcgcggtat cgcctcgaga tcgagaaaga tcctgtcaaa ccggtactcg
    16621 aacacgatca caggctctgc tcccgccacg atacggctgg attcttccgg ataccggccc
    16681 tcgcgcagct cctgccagtc ggtgacgaat ccatgagggg aggcttcccc gggctgctcc
    16741 atgccctcac aggcgaggta ccaggagacg ggtccgggct gggaactcgc gatggtggtt
    16801 tcgccctggg cgcgcagcca ctggagcacc tcggggtagg ccgagcggag ccagaacacc
    16861 tgcacgccgt tgctcagcga ggagctggcc gccacgatca agaccccggc gaccagccag
    16921 tgccgccatt tct
//
LOCUS       DAOPBQ010000015        16670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_3522_length_16670_cov_4.072766, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000015 DAOPBQ010000000
VERSION     DAOPBQ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 16670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16670
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3522_length_16670_cov_4.072766"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PKW60_01165"
     CDS             complement(<1..526)
                     /locus_tag="PKW60_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98070.1"
                     /translation="MQVDYAFAAVLALILVAGAAVAQEGGGTLHAGVAKSDITTNAEG
                     VRINDPLYAKALVLDDGTTRLAIVAMDVTAIGGIGEIGDDFLPALRARIEEELQIPGA
                     HVLVNASHNHPPVRMLCDPPEQLERTFDAVRRAAANMTEVLVGAGAGHEDRISMNRTL
                     HLKDGKAWTIRHTNP"
     gene            complement(579..1331)
                     /locus_tag="PKW60_01170"
     CDS             complement(579..1331)
                     /locus_tag="PKW60_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPJ98071.1"
                     /translation="MTEWSAWQTEIRHREEAILSAARTILLERGYFGLTMDRVAALSE
                     CPKGTMYQHFGCKEDLLVGLAVRYANARLEIMERAAAHRCRSRERVVALGEAVGLYTR
                     LNPEDSRILHMATGIIREKASPLRLNELIQIENREIRLLYEILEDAVREGSLAPQHDQ
                     VLTEMAFGFWALVDGAFTLIESNSPWQVLGIGNPFSNLFRVFSVLADGYGWRPLFAEL
                     DWEESLARARKEVFPQEAQQLYGEGNWYGDRG"
     gene            complement(1781..3562)
                     /locus_tag="PKW60_01175"
     CDS             complement(1781..3562)
                     /locus_tag="PKW60_01175"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPJ98072.1"
                     /translation="MMIALSINLYVACLSATNGTAEPPAFIPAGEFGARIQAVLGRYG
                     NQEIAPRFTDDFILADVVLSPDYPRRFDNFSGDISGRFLGSISMLPNPEQAARLEGLV
                     SEIARHQRPDGRFGTTSLSFAAADIEPENMALLWGNGRLLAGLMEYYQYSGSPQALDC
                     ARKLGDFLIGVYKTCSEPDVAKRLEGEAARGFICFTQLIEGLDMLWRATSDSRYLETA
                     RQIVPLLEPRGVQHTHGYLTTLRGYLMLYESTGDPEILALAEKLYGDLITSSDYLIYG
                     GVLEYFGMKYDRDEGCSEADFLRLSLQLWQATGNLDYLERAERCLLNIFYAGQFETGD
                     FGHRRFDETGYIPAQGAGRAWWCCTMHGHRALADVLRQAVTRDGQRTMIHLYLEGQWS
                     GGGTTLTLARPGATDPSAPVFRITVDAAPGGNSAIALRQPSWAKDIRLRVNGAEQSPT
                     LRDGYLTLERAWKSGDVIEVDWTYAVRLQLRDGSLIQPADLTEQPVQAALFHGPWLLS
                     VIDELDPTFHGEPWRNNVIHLSETVTRPASAGGPSDSPCIVEDAHLLLDYRHDGFPDP
                     CSVILRPISEQTPYRQPMATVWLNFAR"
     gene            4056..4682
                     /locus_tag="PKW60_01180"
     CDS             4056..4682
                     /locus_tag="PKW60_01180"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPJ98073.1"
                     /translation="MAKWRRRPEERPRQILGAALDVFSSKGFARATMDEIAEAAGITK
                     GTIYLYFRSKKDLLLAVIRAELEDFMRLLPSLEGVAPGDLEAHARQLSRAFLNALMAP
                     DAAKTVQVFLSEMHHLPELKRLYLEEALPRANAQMAGFLSAQMDRGALRALDPSIATR
                     CLFGMFFIFVLTQELFGASRVTPMAPERVADTILDIFLHGMLTKGPVS"
     gene            4679..5797
                     /locus_tag="PKW60_01185"
     CDS             4679..5797
                     /locus_tag="PKW60_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPJ98074.1"
                     /translation="MKRVLFILLFLVAGCWNGNDGVLRVAGLLEGIDVKAGTLVGGRV
                     SRVHVTEGSRVSAGDLLVELDPKDTAPMVAAAEARLRQTEAALAKLEAGARPEQLAQA
                     EAAAEQARQQYNMALDGARSQEIDAARAAAEAARAQRDDAQQAFERARRLFETGAASE
                     MAFDQAKNALSGAQAHYTAALEKLDLVAEGARVEEVAMAKAAFDQARALLDELRNGAR
                     VEDIEAARAARDAAKADLELARSRLEEMRVLAPMAGIVETIDVEPGDLVPPGPVVRLI
                     DPDTLELVVYVSAPLLSRIRMGQRIPLLPDGRGAEYEGEVIFIANEGEFMPRNLQTEE
                     SRARQVFGIRLRVPSYDGALKAGMTVTAQIPLNGAAPP"
     gene            5794..6732
                     /locus_tag="PKW60_01190"
     CDS             5794..6732
                     /locus_tag="PKW60_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98075.1"
                     /translation="MTQAIRTENLTRRFGNFTAVDGVNLEIEEGDIFGFLGPNGSGKS
                     TVIRMLCGLLLPTDGSAQVLGYDVVRDSEKVKHSIGYMSQQFSLYPDLTVMENLTFYA
                     RIYGIPRRDRRARIEEVIAGAGIQDYRKRLAGQLSGGWKQRLALACALVHRPRLMFLD
                     EPTAGIDPVARRELWNLLFDLSGQGVTFFVTTHYMDEAERCSHVGYIYYSKLIVRGTP
                     NELKQLEEISPPGTARLEIRCSRLPSAMRILAEKPYVRDVTIFADSLHVLAEQGNETR
                     MAADLEAAGYPQAQVISIPPTLEDVFVTLTQRRKAV"
     gene            6734..7849
                     /locus_tag="PKW60_01195"
     CDS             6734..7849
                     /locus_tag="PKW60_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ98076.1"
                     /translation="MFKGLTSVMYKETFHILRDFRTLFLMLLLPVMDLTIFGYAIDLE
                     VRNIATVVYNLDGRQQSRELLDMFANSGYFTFERVVLSEAELERAIVRGDAKIGLKIP
                     PDYSDRLLRGESAQIQVLLDGSDSTVAMQALQVINAIALRKSIDIISQSANMPGAFPI
                     DARPRVLFNPDMKTPYFMVPGLVGMIMQSVTMLLTAFAVVREKETGTLEQLMVTPVSR
                     LGLMLGKLLPFGVVGFIEMVFALVLMRILFHVPIAGNVVLLGMFGLIFVFATLGMGLL
                     ISTMAQNHMQAVQLALLIMLPSILMSGFMFPIESMPKPLHAVAYAIPATYWIRILRGI
                     SLRAAGFADLWDQGLWMLVIGVVVLAVAVSRFRKTLA"
     gene            complement(7971..10031)
                     /locus_tag="PKW60_01200"
     CDS             complement(7971..10031)
                     /locus_tag="PKW60_01200"
                     /inference="COORDINATES: protein motif:HMM:NF037695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused protease/ribonucleoside-triphosphate
                     reductase"
                     /protein_id="HPJ98077.1"
                     /translation="MLFDVKERLELDPQFLAQYMGRQPDWGPLGYVTFKRTYARPVSG
                     EPDRTEEYWETCRRVVEGCYTIQLTHCRNLKLPWDKQKAQRSAQEMFRLMWDFKFLPP
                     GRGLWMMGTDYIYQRGSAALNNCAFVSTEEIDFEFSEPFCFLMDMSMLGTGVAFDTRG
                     AGKLMVQQPEVDPDYTHVVKDSKEGWVELIRIILNSYAGTCKRPARIDYSQVRPQGSP
                     IRTFGGIAPGPGPLMEGVVNTDKVLSRRIGERITSTDITDLMNVIGKCVVSGGVRRTA
                     ELALGDPCDEAYLKLKDPELFPEELRNWRWASNNSISAELGMDYEAVGQQTAKNGEPG
                     YFWLRNAQAYGRMKDGETWQDAKVAGTNPCAEQSLESYEICNLVETFPSRHETLEEYK
                     RTLKFAYLYAKTVTLVPTHNERTNAIMLRNRRIGLSQSGIIESFERHGRRTHFNWCDE
                     GYKYVCNLDRIYAQWLCVPESIKKTSIKPSGTVSLLPGVTPGIHYAHSEYYLRAMRID
                     KTSKLVEPLRNAGYHIEESVYGDNTWVVYFPARTENFDRSKNDVSVWEQVENVAQMQH
                     YWADNQVSATITFRQEEAQDIPKILELYETRLKSISFLPLSDHQYAQAPYQEISKEMY
                     EHAVSRLRPLDFSNMYTDEAQDLFCDGDKCEIIVPEAVPQTAELEFGEPGASEEHEEV
                     GAPK"
     gene            complement(10053..10835)
                     /gene="thyX"
                     /locus_tag="PKW60_01205"
     CDS             complement(10053..10835)
                     /gene="thyX"
                     /locus_tag="PKW60_01205"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017544788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPJ98078.1"
                     /translation="METVEPRVFLIGETRLVEEGLQTYLQHAGAPAWTTDAPSDAEAL
                     CEVYGRLCYRSFEPGLNPNVTRVRKGNDVYLGHILEVGHGSVLEHAVLNFVFADVSRV
                     FTHELVRHRAGTAISQESLRYVRLSRLSAYVPLQIRESEQGLEIFCRTLEQLEQVQRE
                     LADTFAIDQEGTFAIKKQLTSAFRRVAPLGLATTVGWSCNFRTLRHVIEIRTAPDAEE
                     ELRLVFGQVYKAVRERYPNIFGDYTVEMVDGLPWVKTPHRKV"
     gene            11398..13167
                     /locus_tag="PKW60_01210"
     CDS             11398..13167
                     /locus_tag="PKW60_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98079.1"
                     /translation="MTERRPTAALEDTPVFHPLLLAVCPILGLYAYNVGNVPWTALLR
                     PLIVALVCAQLLQWILWAILRDKHKAGVVASALLLALMWGWSLLETGIAMASRNLQTV
                     EPPVAYGVLGILVFLSALVVYAWNRADKRRALLRAGLFLVIVLLGALTIAVLLDRIYG
                     TVASTFILAFVGLVTGCIVYLVAWRRDFRPWTRTANWFGLILVTLYVALIVFNVPRTD
                     KLPAAAPEMPGLTAQASADSPDIYFILLEGYARCDVLLRLYGHNDLPALEALQDLKMT
                     VESTAFANYPWELQSAISVLNMDYLDHLVPPDSPEDLDIAQFHEFYHRNRFFRTLHDA
                     GYEILAYSPGVQAIEPGPLVDRCLAPPRTPSEFEVVLLQNTACKRLLEVGCYARGRDT
                     RSWQLGFRRARVNYVFDTMGTLAGEPHERPRLVFAHVTVPDPPFIFERKGEPADPRRR
                     RSLEERYLGQLHYVSTRVTDTVQQITERATRPSVILLISAQGPRIRITEDSIEPEALA
                     EHFGVFASTRYPDKAPDAGGPPPKGGSLVNVLRHTLNRVLDADLPLLEDRALLTPMQT
                     PLQSRPVTVPTLEHVVVPALEPR"
     gene            13382..13732
                     /locus_tag="PKW60_01215"
     CDS             13382..13732
                     /locus_tag="PKW60_01215"
                     /inference="COORDINATES: protein motif:HMM:NF028309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit subunit G family protein"
                     /protein_id="HPJ98080.1"
                     /translation="MGKAARTDKSVLERVAQEERLLLLDVAAARTEAQNILEAAQAEA
                     QRILDASEAALTRELADMRRAAEQDAARDRAAIRGAAAAKLAEAQLRAQAHARAVVDD
                     VLQQILPEGRAEAP"
     gene            13729..15657
                     /locus_tag="PKW60_01220"
     CDS             13729..15657
                     /locus_tag="PKW60_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98081.1"
                     /translation="MIARMARLEIVFMRQQLAGMVAFLQEQGLMHLEEVPLAVEQAPG
                     FLHRVHLDEQQKAELQGLEEIDRILRELSPLLGRRIQTAGVEKAARDLSAADLPAWTR
                     LARGWSRELRSLTRRRVNLEDNMAVFKLYRETLRLIETMLGGNQATLGKNGRAFILRG
                     DARLLRRRLLRRFQRELGLQSQFMDAALDRNAVLGLVLYPEERNEAVSRILRDEGIEI
                     IEAPDKRLHGHSVQEITRKTGEAMAEQAIQLKEIQKRLDEYSAEIGMELAALRLLVAN
                     KMEQLRVIGNFAQSEFVAVIQGWVPEREAGALEQALHERFPGEVCVGRLPVERVERKR
                     IPVLLRNHPLLQPFEVLMGLMKPPSYGTLDPSALVGIFFVLFYGYIVGDVVYGVLILA
                     LALGIRRWFGHYPALRSVSMVYIYAAIATIFFGVLFGEYCGDLGMRLEPRLKPLWFPR
                     HHDTVRLLLIAVAFGVAHVVLSLTLAIYSHWYVGDHTHALEKLGMLLGLFAVGVAVAG
                     LSGKLPVNLGVSLGVTGLLLGVALFLFIKTAGALAPVHVLEVISLTSNVMSYSRLMAL
                     GIASVAIAGVANSLSRGAEALWVAIPLFILIHLFNIALSLFSPTLHSLRLNYVEFLPK
                     FYSPGGRSYKPFRKETLW"
     gene            15651..16010
                     /locus_tag="PKW60_01225"
     CDS             15651..16010
                     /locus_tag="PKW60_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HPJ98082.1"
                     /translation="MVTGRSWGTFALAGVLAVLAAVAAPAAFAAEADGAVAKKEITMP
                     EAVRTVGLALGAAIAVAGAALATARVQAAVGAGGTGALVEKPDLFVTILVLIAIPETL
                     MVFGFVIAILLWQLIGT"
     gene            16007..16621
                     /locus_tag="PKW60_01230"
     CDS             16007..16621
                     /locus_tag="PKW60_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HPJ98083.1"
                     /translation="MSAVEQHSSPAAAFFETMKREARERCESIRDKARDEAQRIVDAA
                     RRAASESREKTLEEGRHNADAHRVDIRRRAQSEAERASMTMREQVVDRILDEVHEALS
                     AIVERADFPALVCRLLAEVMEETPGEPVVLAPPAHTAACRQWLDAHGRAAVPVEADER
                     LWDGVAVTDSARSFRLTNTLTGRFEKSRATARKAALHRLFGGQP"
BASE COUNT         2995 a         5176 c         5198 g         3301 t
ORIGIN      
        1 aggggttcgt gtgccgaatc gtccaggctt tgccgtcttt caagtggagc gtccggttca
       61 tagatattcg atcttcatgc ccggcgcccg cccccaccag cacctctgtc atgtttgccg
      121 cggcgcggcg caccgcatca aaggtgcgct ccagctgttc gggcggatcg cacagcatcc
      181 gcaccggcgg atggttgtgc gaggcattca cgagcacgtg ggcgcccgga atctgcagtt
      241 cttcttcgat ccgtgcccgc agggccggga gaaaatcgtc cccgatttcc ccaatccctc
      301 cgatggccgt cacgtccatg gcgacaatgg cgagccgcgt cgtcccatcg tccagcacaa
      361 gggccttggc atacagggga tcgttgatgc gcacgccctc ggcattggtg gtgatatcgc
      421 tttttgccac acccgcatgc aatgtcccac cgccctcttg cgcaacggcc gcacccgcca
      481 ctaaaatcaa cgccagcacc gcagcaaacg cgtagtcgac ctgcattcaa aaaactcccc
      541 ggggaacgcc gtctgggtat accggtgctt cggtggcttc agccgcggtc cccgtaccag
      601 ttgccttcgc catagagctg ctgcgcctct tgcggaaaca cttccttccg ggcgcgggcc
      661 aggctctctt cccagtccag ttccgcaaac agcggccgcc agccatagcc gtccgcgaga
      721 acagaaaata cgcgaaacag attggaaaac gggttgccga tacccaggac ctgccacgga
      781 ctgttgcttt cgatcagcgt gaaagcgccg tccacgagtg cccagaaccc gaacgccatc
      841 tccgtcagca cctgatcgtg ctgaggcgcc aggctgcctt cccgcaccgc gtcctccagg
      901 atttcgtaga gcagccggat ctcgcggttt tcaatctgga tcagttcgtt cagccgcagt
      961 ggcgaggcct tttcccggat gatccccgtc gccatgtgca gaatgcgcga gtcctccggg
     1021 ttgagccggg tatagagccc caccgcctct ccgagcgcca ccacgcgctc acgcgacctg
     1081 caacggtgtg ccgccgcgcg ctccatgatc tccaggcggg catttgcgta acgcaccgca
     1141 agccccacca gcagatcctc cttgcatccg aaatgctggt acatcgtacc cttcgggcat
     1201 tcgctcagcg cggcgacccg gtccatggtc aacccgaaat atccccgctc cagcaatatc
     1261 gtccgcgcgg cagagaggat cgcttcttcg cgatgacgga tctctgtttg ccatgcagac
     1321 cattcggtca ttgcaaagtt ctcccgcagg ttgcccgaca ctccagtatc atatcacatc
     1381 aaaacggccg tgccgccagc ggacagatcg cgttatctga acatttccgg tgatatcgtc
     1441 atcctgcttt cggtcgtacg ggaacgtgaa aggcagaaca aagtcaggag cgcgattttt
     1501 ttggggatgg agcacgtctg aggaagggat ggtgggaatc caccgcaggc ccggccatgt
     1561 accccgtcca caccgcctac gcggtccaca ttctcatcca ctgaggctct cgccagccca
     1621 ctactgtcca cccccggcca cccgcgccgc gttgggcaac gcactcggcc tccaccaggc
     1681 aggtcttcac gttgtcgaac aggcgttccg gcgattaccc cccggggcgg cctctgccgc
     1741 cgtcccgggg gcggaccctc gcgcaggcct cggataagag ctaccgggcg aaattgagcc
     1801 acacggtcgc cataggctgg cggtaggggg tttgctcaga gatgggccgc aggatgacgg
     1861 agcagggatc ggggaagccg tcgtggcggt aatccaggag caggtgcgca tcctccacga
     1921 tgcacgggga gtccgaagga ccccctgccg atgccggccg cgtcacagtt tcggacagat
     1981 gaatgacgtt gttgcgccag ggttccccat ggaatgtcgg atcgagctca tcaatcacgc
     2041 tcagcagcca ggggccgtga aacagcgcgg cctgaacggg ttgctctgta aggtcagcgg
     2101 gctggatcag cgagccatcc cgcagctgca ggcgcacggc gtaggtccag tccacttcga
     2161 tgacgtcgcc gctcttccag gcgcgctcga gggtgaggta gccgtcgcgc agggtcggtg
     2221 actgttccgc gccattcacg cgcagccgga tgtcctttgc ccaggacggc tgccgcaggg
     2281 cgatggcgct gttgcctccg ggggcggcat cgaccgtgat gcggaacacg ggcgcgctcg
     2341 gatcggttgc gccggggcgg gccagcgtca acgtggtgcc gccgccggac cactgacctt
     2401 caagatagag gtggatcatc gtgcgctggc cgtcgcgggt cactgcctgg cgcagcacat
     2461 cggcgagcgc gcggtgaccg tgcatggtgc agcaccacca ggcgcgcccg gcgccctgcg
     2521 cggggatgta tccggtctca tcgaatcggc ggtggccgaa atcgcccgtc tcgaactgtc
     2581 cggcatagaa aatgttcagc aggcagcgct ccgcgcgttc caggtagtcc aggtttccgg
     2641 tggcctgcca gagctggagg ctcagccgca gaaaatcggc ctccgaacag ccttcgtcgc
     2701 ggtcgtattt catcccgaaa tactccagaa ccccgccgta gataaggtaa tcgctcgatg
     2761 tgatgagatc gccatagagt ttctcggcca gggcgaggat ctccggatca ccggtcgatt
     2821 cgtagagcat gagatagccg cgcagggtgg tcaggtaccc gtgcgtgtgc tgcacgccgc
     2881 gcggttccag caacggcaca atctgtctcg cggtttcgag gtaacggctg tcggaagtcg
     2941 cccgccagag catatccagc ccttcgatca actgcgtgaa acagatgaag ccgcgcgcag
     3001 cctcgccttc cagccgtttt gccacatcgg gctccgagca ggtcttgtag acgccgatga
     3061 gaaagtcgcc gagcttccgg gcgcagtcca gggcctgcgg ggagccggaa tattggtaat
     3121 actccatcaa tcccgccaac agacggccat tcccccacag cagggccata ttctcaggtt
     3181 cgatgtccgc agcagcgaac gacaggctcg tggtaccgaa acggccgtcc ggccgctgat
     3241 gccgggcgat ctcgctcacg aggccttcca gccgcgcagc ctgctccgga ttcggcagca
     3301 tcgaaatcga gcccagaaag cggccggaga tatcgccgct gaaattatcg aaacggcgcg
     3361 ggtagtccgg cgacagcacc acgtcggcca ggatgaagtc gtccgtgaaa cgcggggcaa
     3421 tctcctggtt gccatagcgg ccaagcacgg cctgaatccg cgcgccaaat tcgcccgccg
     3481 ggatgaatgc gggcggttcc gcggttccgt tggtcgcgga caggcaggct acgtacagat
     3541 tgatggagag cgcgatcatc atggtcagat tccttccggt acaggctcag ggttccagcc
     3601 gctgcatgat acggaaggcc gtcttcccgc tcaagatgcg ctggcacatt caagttctaa
     3661 atgcattcaa agcgattttt cgtttctgca ggaagatttc attgggtgtt cggtatccga
     3721 ggcatttttg gggccggttg ctcagttcat cgactatttc ctggagtttt tctaatgtag
     3781 gggcaggccc ctgtgcctgc ccatccggat agcccccgtg gcggcgctga agaagggcag
     3841 acacaggggt ctgcccctac gcactgcgga ggtcagtctt cgatgtcttc ttcctttcca
     3901 gcgcctcgct gaagactcac ccacagaaaa aggactcaac cccaaaaccg cgcatttcgc
     3961 attagaatgc gcgggcagca gatggagcgt gacacgaggc gcggcccgtg gtattcttag
     4021 taactgacca tttagttagt aatagggagc gggtcatggc gaagtggcgg cggcgtcccg
     4081 aagaacggcc ccggcaaatc ctcggcgcgg cattggacgt tttctcgtcc aaagggtttg
     4141 cccgtgcgac gatggatgag attgcagagg cggcgggcat cacgaaaggc acgatctacc
     4201 tgtactttcg gagcaagaaa gaccttctgc ttgcggtaat ccgcgcggag ctggaggatt
     4261 tcatgcgctt gctgccctct ctggagggag tcgcgcccgg cgacctcgaa gcgcacgccc
     4321 gccagttgag ccgcgccttt ctcaacgccc tgatggcgcc ggatgcggcg aaaacagttc
     4381 aagtcttcct gtcggaaatg caccacctgc ccgaattgaa acggctctat ctggaagaag
     4441 cgcttccgcg ggcaaacgct caaatggcgg ggtttctctc tgcacagatg gaccgtgggg
     4501 ccctgcgtgc gctggatccg tccatcgcga cgcgctgcct gtttgggatg ttcttcatat
     4561 ttgtgctcac ccaggagctg ttcggcgcga gccgggtgac gcccatggcg ccggagcggg
     4621 tggcggacac gatcctcgac atttttctcc acggaatgct cacgaaaggt ccggtgtcat
     4681 gaaacgcgtg ctgttcatac tgttgttttt ggtggccggc tgctggaacg gcaacgacgg
     4741 cgtgctgcga gtcgccggcc ttctggaagg catagacgtg aaggccggca cattggtggg
     4801 cggccgggtt tcccgggtcc atgttacgga ggggagccgc gtgtcggcgg gggatctgct
     4861 ggtggaactg gatccgaaag acacggcgcc gatggtggcg gcggccgagg cccggttgcg
     4921 tcagacggag gccgccctgg cgaaactaga ggccggggcg cggccggaac agctggcgca
     4981 ggccgaagca gcggccgagc aggcccgcca gcaatacaac atggcgctgg acggggcgcg
     5041 atcgcaggag atcgacgccg cacgggccgc ggccgaggct gcacgcgcgc aacgcgacga
     5101 tgcccagcag gcgtttgaac gggcgcggcg actgttcgaa accggcgcgg catcggagat
     5161 ggcctttgac caggctaaaa atgccttaag cggcgcgcag gcccactaca ccgccgcgct
     5221 ggaaaagcta gatctagtcg ccgagggggc gcgcgtggag gaagtggcca tggccaaggc
     5281 cgcgttcgac caggcccggg ccctgctgga tgaattgcgg aacggcgcac gcgtggagga
     5341 tatcgaagct gcccgcgcgg cccgggacgc agcgaaggcc gatctggagc tggcccggag
     5401 ccgcctggag gagatgcgtg ttctcgcgcc gatggcgggc atagtcgaaa ccatcgacgt
     5461 tgaaccgggc gatctggtgc cgccggggcc ggtggtacgc ctcattgacc ccgacacgct
     5521 cgaactcgtg gtttatgtca gcgcgccgct gctgtcccgg atccgcatgg gacaacgcat
     5581 tccgctattg cccgacggcc ggggcgcaga gtacgaggga gaggtgatct tcattgcgaa
     5641 tgaaggggaa ttcatgccgc gcaacctgca gacagaggaa tctcgcgcgc gtcaggtgtt
     5701 cggcatccgg ttgcgcgtcc cgtcgtatga cggggcgctg aaagcgggaa tgaccgttac
     5761 agcccagatc ccgcttaacg gtgcagcgcc gccatgaccc aggccattcg caccgaaaac
     5821 ctgacccgcc ggttcggcaa ttttactgcc gtggacggcg tgaacctcga gatcgaagag
     5881 ggcgacatct ttggttttct cgggccgaac ggctcgggga agtcgacggt gatccgcatg
     5941 ctgtgcggtc ttctgttgcc aacagacggc agcgcgcagg tgctgggcta tgatgtggtc
     6001 cgcgacagtg agaaggtgaa acacagcatc gggtacatgt cgcagcaatt cagcctgtat
     6061 ccggatctga ccgtgatgga aaacctgacg ttttacgcgc gtatctacgg aattccgcgc
     6121 cgcgaccgcc gggcgcgcat tgaagaggtg atcgccggcg cgggcatcca ggattaccgc
     6181 aaacggcttg cgggccagct ttcgggcggc tggaaacagc gtttggccct cgcctgcgcg
     6241 ctggtgcacc ggccccggct catgtttctc gatgaaccca cggcgggcat tgatccggtg
     6301 gcccggcgag agctctggaa tctcctcttc gacctttcgg gccagggggt gaccttcttt
     6361 gttaccacgc attacatgga tgaggcggaa cggtgcagcc acgtggggta tatctattat
     6421 tcgaagctca ttgtccgcgg tacaccgaac gaattgaagc agctcgagga gatctcccca
     6481 cccggcacgg cccggctgga gatccggtgt tcccggctgc cctcggccat gcggatcctg
     6541 gcggaaaaac cctatgtgcg cgacgtgacc atttttgcgg actcattgca tgtgcttgcc
     6601 gaacaaggca atgaaacccg gatggcggct gacctcgaag ccgccgggta tccacaggcc
     6661 caggtgatct ccattccacc gacgctcgag gacgtgttcg tgaccctcac ccagcgcagg
     6721 aaggccgtct gagatgttta aagggctcac ttccgttatg tacaaggaga cgttccacat
     6781 cctgcgcgat tttcgcacgc tgtttctgat gctgctcctg ccggtaatgg acctgacaat
     6841 tttcgggtac gcgatcgatc tcgaagtgcg caacatcgcc accgtggtct acaacctcga
     6901 tgggcgccag caaagccggg aattgctcga tatgttcgcc aattccggtt atttcacgtt
     6961 tgagcgcgtg gtgctgagcg aggcggaact cgaacgggcc atcgtacggg gtgacgccaa
     7021 gatcggcctg aagatcccgc ccgactacag cgatcgtctg ttgcgcgggg aaagcgccca
     7081 gattcaggtg cttctcgacg gcagcgattc caccgtggcc atgcaggcgc tgcaggtcat
     7141 caacgccatc gccctgcgta aatccatcga catcatttcg caaagcgcga acatgcccgg
     7201 cgccttcccg atcgatgcgc ggccccgggt cttgttcaat cccgacatga agactccgta
     7261 tttcatggtg ccggggcttg tgggcatgat catgcagtcg gtgaccatgc tcctgacggc
     7321 gttcgccgtg gtgcgcgaaa aggagacagg cacgctggag caactcatgg tgacgccggt
     7381 gtcgcgcctg ggcctcatgc tcggcaagct gctgccgttc ggcgttgtcg ggtttatcga
     7441 gatggtcttc gcgctggttc tgatgcggat cctgttccac gtgcccatcg ccggaaacgt
     7501 ggtcctgctg gggatgttcg ggctgatttt cgtgtttgcg acgctgggca tgggcctgct
     7561 catctcgact atggctcaga atcacatgca ggccgtgcag ctggcccttc tgatcatgct
     7621 gccttccatt ctgatgtcgg ggttcatgtt ccccattgaa tccatgccca aacccctgca
     7681 cgcggtggcc tatgcaatcc ccgcaaccta ctggatccgg attctccgcg ggatcagcct
     7741 gcgggcggcg ggcttcgccg acttatggga ccagggcctg tggatgctgg tgattggggt
     7801 ggttgtgctg gccgtggcgg tttcccggtt ccgaaagacg ctggcctgag ccctgcattt
     7861 cgccagcagc accgagcggc gcacgcgcaa cacctggcgc catatactga cgccggggcc
     7921 cgtgggcgcg gaaagacggt ccccgatccc cgctcgagat cggagcgccg ctatttcggc
     7981 gcgccgactt cctcgtgttc ttcgcttgcg cccggctcac cgaattcgag ttcagcggtc
     8041 tgaggcaccg cctcgggtac gatgatctcg cacttgtcgc cgtcgcagaa gaggtcctgc
     8101 gcctcatcgg tgtacatgtt ggagaagtcg agcggtctca ggcgcgacac cgcgtgctcg
     8161 tacatctctt tcgagatctc ctggtagggc gcctgcgcat actggtggtc gctcagcggc
     8221 aggaaactga tggatttcag gcgcgtctcg tacaattcca ggattttggg gatgtcctgc
     8281 gcctcttcct gccggaacgt gatcgtggcg ctgacctgat tgtccgccca gtagtgctgc
     8341 atctgggcga cgttttcgac ctgttcccag actgagacgt cgtttttcga acggtcgaaa
     8401 ttttcggtgc gcgcggggaa gtacaccacc caggtgttgt cgccgtaaac cgattcctcg
     8461 atgtgatagc cggcgttgcg caacggttcg accagtttgc tcgtcttgtc gatgcgcatc
     8521 gcccgcagat agtactcgga gtgtgcgtaa tggatgcccg gggtcacgcc tggaagcagc
     8581 gataccgtgc cgctgggttt gatgctggtc tttttgatgc tctccggcac gcacaaccac
     8641 tgcgcgtaga tgcggtccag gttgcagacg tatttgtaac cttcgtcgca ccagttgaaa
     8701 tgcgtgcggc gcccgtgccg ttcgaaggac tcgatgatgc cggattgcga caggccgatg
     8761 cggcgattgc gcagcatgat cgcattggtg cgttcgttgt gcgtcggcac cagtgtgacg
     8821 gtcttggcgt acaggtacgc aaatttcagc gtgcgtttgt attcttccag tgtctcgtgc
     8881 cgcgaaggaa aggtctccac cagattgcag atttcatacg actcgaggct ttgctcggca
     8941 cagggattcg ttcccgccac cttggcatcc tgccaggttt cgccgtcttt catccggccg
     9001 taggcctgcg cgttacgcaa ccagaaatag ccgggctcgc cgtttttcgc ggtctgctgg
     9061 cccacggctt cgtaatccat tcccagttcc gcggagatgc tgttgttcga ggcccaccgc
     9121 cagttccgca gttcctcggg gaacagctcg gggtctttga gtttgagata ggcttcgtcg
     9181 caggggtcgc ccagagccag ctccgccgtc cggcgcacgc cgcccgacac aacgcatttc
     9241 ccgatcacgt tcatcaggtc ggtgatgtcg gtcgacgtaa tgcgctcgcc aatacgccgc
     9301 gacaacacct tgtccgtgtt cacgacccct tccatcagcg gtccggggcc gggcgcaatg
     9361 ccgccgaagg tccggatcgg ggagccctgc gggcgcacct gtgaataatc gatccgggcg
     9421 gggcgtttgc aggtgcccgc gtacgaattc aggatgatgc ggatcagctc cacccatccc
     9481 tctttgctgt ctttgaccac gtgggtgtag tccggatcca cctccggctg ctgcaccatc
     9541 agtttgcccg cgcccctggt gtcgaaggcc acgccggtgc ccagcatcga catgtccatc
     9601 aaaaaacaaa acggttccga gaactcgaaa tcgatctctt ccgtcgacac aaacgcgcag
     9661 ttgttcagcg ccgccgaacc gcgctggtag atgtagtccg tgcccatcat ccagaggccg
     9721 cgtccggggg ggaggaattt gaaatcccac atcagccgga acatttcctg ggccgagcgc
     9781 tgggctttct gtttgtccca gggcaatttg agattccggc aatgggtcag ttgaatggtg
     9841 tagcaccctt ccacgacccg ccggcaagtt tcccagtatt cctccgtgcg gtccggttcg
     9901 ccggacacgg ggcgggcgta cgtacgcttg aaggtcacat agccgagcgg cccccaatcc
     9961 ggctgccgtc ccatgtactg cgccagaaat tgcgggtcca gttcaagccg ctctttcacg
    10021 tcgaatagca tgtccccaga ttcctctttg tattagacct ttcgatgggg agttttgacc
    10081 cagggcaggc cgtccaccat ctcgactgta taatccccaa agatgttcgg ataccgctcc
    10141 ctgaccgcct tatagacttg tccaaacacc aggcgcagtt cttcctccgc atccggcgcc
    10201 gtgcgaattt caatcacgtg gcgcagggtg cggaagttgc acgaccagcc cacggtcgtc
    10261 gccaggccca ggggcgcgac gcgccggaag gcggaggtga gctgtttttt gatcgcgaac
    10321 gtcccttcct ggtcgatggc gaacgtatcg gccagttcgc gctgcacctg ctccagctgc
    10381 tccagggtgc ggcagaagat ctcgaggcct tgctcgctct cgcggatctg caggggcaca
    10441 tacgccgaca ggcggctcag gcggacgtag cgcagcgact cctgggagat cgccgtgcct
    10501 gcgcggtgcc gcaccagctc atgcgtgaac acgcggctga catccgcaaa cacgaagttc
    10561 aacaccgcat gttccagcac gctgccgtgg ccgacctcga ggatgtggcc gagatacacg
    10621 tcgttgcctt tgcgtacccg cgtgacattc ggattcagcc ccggctcgaa tgaccggtag
    10681 cagaggcggc catacacctc gcacagcgcc tcggcatcgc tgggtgcatc ggtagtccat
    10741 gccggtgcgc ccgcgtgctg caaataggtc tgcaggccct cctcaacaag ccgcgtttct
    10801 ccaatgagaa agactctggg ttccaccgtc tccatagatt agcgcctctt tccggacttc
    10861 aatgacaacc acaatctgtt gacaacaaat aacttaaaga agacttccca tgcgacggga
    10921 tccgctgttg cggcacccat acttccttcc ccgtgatgct gcattatagg aatgggcgcg
    10981 gccattgtcc attgcatttc tgagcctgcc tcccgccttc cccgctccgg cgaattcgcc
    11041 acaggcacgt atctcctcac ggcaggatgc ggatccagca gcagacgtgg ctgtcttcca
    11101 gcgcctccag ggaactcccg cctgaactgt tcctcaaatg ccccctgggt acgtccgcac
    11161 agcgcctggc gggatcttgg tttgatcggc gccaggaggt ccccaaatcg ctatatgtag
    11221 tacagttttg ataacaacca ccccatataa agtatttctc tggtgggtga agtgtcccaa
    11281 ggatttgccg gtttgtcaag gtgttttagc ggggactggg atagatacag gcttgacaat
    11341 ggcttacata gaaacgatga ttaacgtgaa atggaaaatg aagagcactt ggaagccatg
    11401 actgaacgaa gacccacggc ggccctggaa gacaccccgg ttttccaccc gctgctgctg
    11461 gctgtctgcc ccattctggg cttgtacgct tacaacgtgg gcaacgtgcc atggacggcc
    11521 ctgctccgtc ccttgatagt cgccctggta tgcgcccaat tattgcaatg gattctctgg
    11581 gcaatcctga gagacaagca caaggccggg gttgtggcct cggcgctttt gctcgccctg
    11641 atgtggggct ggagcctcct ggagaccggc attgccatgg cctcccgcaa cctgcaaacg
    11701 gtggagccgc cggtggccta tggcgtgctt gggatcctgg tgtttttgag cgcactggtg
    11761 gtttatgcct ggaaccgcgc agacaagcgc cgcgccctgc ttcgggccgg cctgttcctg
    11821 gtgatcgtac tcctcggcgc gctgacaatc gccgttctgc tcgatcggat ttacgggacg
    11881 gttgcgtcga ccttcatcct cgcgttcgtg ggtctcgtca ccggctgcat cgtctatctg
    11941 gtggcgtggc ggcgtgattt tcgcccgtgg acccgcacgg cgaactggtt cggtctcatc
    12001 ctggtcaccc tgtacgttgc cctgatcgtg tttaatgtcc cgcgcaccga caagctgcct
    12061 gcggctgcgc cggagatgcc cggcttgact gctcaggcct ccgcggattc cccagacatc
    12121 tatttcatac tgctggaggg ctatgcgcgc tgcgatgtcc tccttcggct ctacggccac
    12181 aacgatctcc cggcacttga agcgctccag gatctcaaga tgacggttga gagcacggcg
    12241 ttcgccaatt atccctggga acttcagtct gcgatctccg tgcttaacat ggactacctg
    12301 gatcacctgg tgccgccgga ttctcccgag gatctcgaca tcgcacagtt tcatgagttc
    12361 taccaccgga accgtttttt ccggacgctg catgatgccg gttatgaaat tctggcctat
    12421 tcgccggggg ttcaggccat cgagcccggg ccgctggttg accgctgcct ggcaccgccg
    12481 cgcaccccct ccgaatttga agtggtgctg ctccagaaca cggcgtgcaa acgtctgttg
    12541 gaagtgggct gttacgctcg cggccgggat acccgttcct ggcaattggg atttcgccgg
    12601 gcgcgggtca attatgtctt cgacacgatg ggcactcttg cgggagagcc ccatgagcgg
    12661 ccgcgtctcg tgtttgcgca tgtcacggtt cccgatcccc cgtttatctt tgaacgcaag
    12721 ggcgagcccg cggacccgcg ccgccgccga tcgcttgagg agcggtatct gggccagttg
    12781 cactatgtca gcacgcgggt gaccgacaca gttcaacaga tcaccgaacg cgccacgcgg
    12841 ccctcggtca ttctgctcat ctccgcgcag ggcccccgca ttcgcatcac cgaggactcc
    12901 atcgagccgg aggcgctggc cgagcatttc ggggtgtttg ccagcacacg ctaccccgac
    12961 aaggcgccgg atgccggagg cccgccaccg aaagggggca gcctggttaa cgtgctgcgg
    13021 cataccctga accgggttct ggacgccgat ctgcctctgc tggaagaccg ggccctgctg
    13081 acgccgatgc agaccccgct ccagtctcgc ccggtcaccg tgccgacgct tgagcatgtc
    13141 gttgtgcccg cgcttgaacc ccgatgatgc cgatggtact ctgcggcgtc cgggcaggcg
    13201 ccggtaatac cctgccgccg ccgactggct gcgtacgcaa atcaggcata tcgcgccggc
    13261 ccgtgcacgt gttactatgt tcgcacctca tccataaacg agagatcgcc ggagttgccg
    13321 gctgccagcg ttgccgggaa gaaggacttt gcgggaacgg aggcaatgca gcaggagtac
    13381 ggtgggtaag gcagcgagaa cggacaaatc ggtgttggag cgcgtggccc aggaagagcg
    13441 tctgctgctg ttggacgtag ccgccgcccg caccgaagcc caaaacatac tcgaggcggc
    13501 ccaggcagag gcccaaagaa tcctggatgc cagcgaggcg gcgctgacgc gcgagcttgc
    13561 ggacatgcgc cgggctgctg aacaggacgc ggcgcgggat cgtgcggcga tccggggagc
    13621 cgccgccgcg aaactggccg aggctcagtt gcgggcgcag gcccacgcgc gcgcggtcgt
    13681 tgacgacgtg ctgcagcaga tccttcctga aggccgagcg gaggctccat gatagcccga
    13741 atggcgcgcc tcgaaattgt gttcatgcgg cagcagctgg ccgggatggt ggcctttctg
    13801 caggaacaag gcctgatgca tctcgaagaa gtgccgctgg ccgtcgaaca ggccccgggc
    13861 ttcctgcacc gcgtgcacct ggacgagcag cagaaggccg aactccaggg cctcgaagaa
    13921 attgacagaa tcctcaggga actctcccct cttcttggcc ggcgcatcca gacggccggc
    13981 gtggaaaaag ccgcgcgtga tttaagcgcc gccgatctgc ccgcgtggac ccgcctggca
    14041 cgcgggtgga gccgggaact ccgctcgctc acccggcgcc gtgtgaatct cgaagacaac
    14101 atggccgtct tcaagctcta ccgcgagaca ctccgcctga ttgagaccat gctcggcgga
    14161 aaccaggcga ccctcggcaa aaacggccgc gccttcattc tccggggcga tgcccgcctc
    14221 ctgcgccgcc gcctgctgcg gcgtttccag cgtgagctcg gactgcagag ccagttcatg
    14281 gatgcggccc tcgaccggaa cgcggtgctg ggcctcgtgc tctatccgga ggagcgcaat
    14341 gaggcggtga gccgcattct gcgcgacgaa ggcattgaaa tcatcgaggc gcccgacaaa
    14401 cgtctgcacg ggcactccgt tcaggagatc acccgcaaga ccggagaggc gatggcggag
    14461 caggcgattc aattgaagga aattcagaaa agactggatg aatacagcgc tgaaatcggc
    14521 atggaactgg ccgcgctccg gctgctggta gccaacaaga tggagcagtt gcgcgtgatc
    14581 ggcaattttg cccagagcga atttgtggcc gtcatccagg gctgggtgcc cgagcgtgaa
    14641 gcgggcgccc tcgaacaggc gctccacgag cgtttccccg gcgaggtttg cgtggggagg
    14701 ctgccagtcg agcgcgttga gcgcaaacgg attccggtcc ttctgcggaa ccatcccctg
    14761 cttcagccgt tcgaggtgct catgggcctg atgaagccgc cgtcctacgg caccctggat
    14821 cccagcgcgc tggtgggcat ctttttcgtg cttttctatg gatacattgt tggcgacgtg
    14881 gtgtacggcg tgttgatcct ggcgctcgcg ctggggattc gccggtggtt cggccactat
    14941 ccggccctgc gcagcgtcag catggtgtac atctatgcgg ctatcgcgac gatatttttc
    15001 ggagtgcttt tcggtgagta ctgcggcgat ctcggcatgc gcctggaacc gcgcctgaaa
    15061 cctctgtggt tcccgcgcca ccatgatacc gtgcgcctgc tgctgatcgc ggtggcgttt
    15121 ggcgtggcgc acgttgtgtt gagcctcacc ctggccattt acagtcactg gtacgtgggc
    15181 gaccacacgc acgccctcga aaagcttggc atgttgctgg gactgttcgc ggtgggcgtg
    15241 gctgttgccg gcctgtccgg aaagctgcct gtgaacctgg gcgtcagcct gggcgtgacg
    15301 ggactgcttc tcggcgtagc gttgtttctg tttatcaaaa ccgcgggcgc tctggcgccg
    15361 gtccatgtcc tggaggtcat ctcgcttacg agcaacgtga tgtcatacag ccggctcatg
    15421 gcgctcggca tcgcatcggt ggcgattgcc ggtgtcgcca acagcctgtc gcggggcgct
    15481 gaagccctct gggtggccat tccgctgttt attctcattc atctgttcaa catcgcactc
    15541 agcctcttca gcccgacgct gcactcgctg cgtctgaatt atgtggaatt cctgcccaag
    15601 ttctattcgc ccggaggcag aagttataaa ccgttcagga aggagacact atggtaaccg
    15661 gacgcagttg gggcacgttc gcgctcgcgg gagtccttgc ggttctagcg gccgtggcag
    15721 cgccggcggc ctttgccgcg gaagcggacg gcgccgttgc caaaaaggaa atcaccatgc
    15781 cggaggccgt gcgcacggtc gggctggcgc tgggcgccgc catagcggtt gccggggcgg
    15841 cgctcgccac ggcgcgcgtg caggcggcgg tcggcgccgg aggcaccggg gcccttgtcg
    15901 aaaaacctga cctttttgtg accatcctgg tgctgatcgc catcccggag acgctcatgg
    15961 tcttcggctt cgtgatcgcc atccttttgt ggcagctgat tggaacgtga gcgctgtaga
    16021 acaacatagc agtcctgccg cggcgttttt cgagaccatg aagcgcgaag cccgcgaacg
    16081 ctgcgagtct atccgcgaca aggcccggga tgaggcgcag cgcattgtcg atgcggcccg
    16141 gcgggccgcc agcgagagcc gggagaaaac gctcgaggaa gggcgccaca acgcggacgc
    16201 ccatcgcgtc gacatccgcc ggcgcgcgca gtctgaagcc gaacgcgcgt ccatgaccat
    16261 gcgcgagcag gtcgtagacc gcattctgga tgaggtgcac gaggcattgt ccgccatcgt
    16321 agagcgggcc gattttccgg cgctcgtatg ccgccttctg gcggaagtga tggaggaaac
    16381 gcccggggaa cccgtcgtgc tcgcgcctcc ggcccatacc gccgcgtgcc gccagtggct
    16441 ggatgcgcac gggcgggcgg ccgtgcccgt cgaagctgac gagcgcctct gggacggcgt
    16501 ggccgtcacg gattcggccc gctcgttccg cctgaccaac acgctcaccg gccggttcga
    16561 aaaaagccgc gccacggccc gaaaagctgc gctccaccgg ctctttggag ggcagccgtg
    16621 aactatcgcg gtgaaaacgt gcgcgagcac atcgtctaca tcgccacccg
//
LOCUS       DAOPBQ010000016        15866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_3764_length_15866_cov_3.463918, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000016 DAOPBQ010000000
VERSION     DAOPBQ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 15866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15866
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3764_length_15866_cov_3.463918"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /gene="dinB"
                     /locus_tag="PKW60_01235"
     CDS             <1..1206
                     /gene="dinB"
                     /locus_tag="PKW60_01235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPJ98084.1"
                     /translation="AMERHILHIDMDAFFASVEQVLNPALVGKPLIVGGLKSDRRGVV
                     STASYEARKYGVHSAMPLIEAKRRCPHGIFIRGRFEHYRKASEQIRAILERVSPCVEM
                     ASIDEAYVDITGSQRLFGGDDAIARYIKAAIRAETRLPSTIAIAPNKLVAKIASGEGK
                     PDGYLRINAERVPSFLRPLPLAKLPGAGPRTREILERLGIMTVGSLADVPVDVLVQAF
                     GSMGYALQRAARGESTAPVQPCSRPKSISRETTFEEDLLDWERIERIVAYLAERCAHA
                     LRENGMETRCVTLKVRHSDFSTYTFSKTLPESTCLERDIGEALGGLLPKARQRRARLR
                     LVGVALTSLSYDQRQLRLFDGKEPEKWLRVMESVDRIRARHGFEFIRFGKSMELGRHV
                     ELATPSLSR"
     gene            1365..4448
                     /locus_tag="PKW60_01240"
     CDS             1365..4448
                     /locus_tag="PKW60_01240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPJ98085.1"
                     /translation="MFTALIPLHVHSNYSMLEGTAPVERLVERAASFGLPALALTDTG
                     GLYGAVPFYRIARAAGIKPIIGTFLENTVLLARDREGYAQLCRLVSAYHLEESFDLTA
                     CLSECSGVFALTDDAGRIRRLHRNGLDPLVALTHHGDAVSRYRATRLRDLAAELGLRC
                     VAANPVYFLQPEEYRLHRVLSAIRCNATVDTLEAGSAAGAACWFRPPEEVRRLYADWP
                     DALRNAEWVAEECCLELPLGTPLFPEFKLPGGETPFSYLWKLAFDGVKQRYMPLNRRI
                     IERLHYELDIIERLGFAPYFIIVWDIVHFARSRGIPVVGRGSAANSLVAYALGITRVD
                     PFKYDLYFERFLNPSRSDCPDIDLDICWRRRDQVVDYVYQRYGAGHVAMIATFNTFQA
                     RSSVREIAKAMGLTGSEIGEVTRRLPRYHAGDIQTVVRWLPECRELPIHEEPLRSIVA
                     IAEAIDQYPRHISLHPCGLVIAPEPLTRFVPLQRTAKGPVVTQYDMHPIEELGLIKID
                     LLGHRSLTVIDETVQQVWDNRGIRLDVETLPEPDPCTADLIRNGRTIGCFQIESPAMR
                     TLLRNTQAADTDMLIKTLSLVRPGPSGSGMKKRFIARHLGKEPVEYLHPALERVLSDT
                     CGVMLYQEDILKVANVIAGMDLTEGDMLRRAMTKKRSPREMAKSMKGFIEKAVANGIA
                     EDVAGEIWGLIANFAEYAYCKAHAATYGEIAYQCTYLKAHFPAEFMACVLSNRGGFYH
                     PAVYVEEARRLGIEVRPPDINKSRFEYAAEGRAIRMGFVEVRQLAQKTVDAILAGRAK
                     RPFESLADVWSRTGMSRPEAEALIQAGAFDAFGRTRPALFWELHLLAQGGEREEPAGA
                     ARLFREHGGEYRTVPRLPDVSRKKRTGQEWEALGLMVSTHPLEYYAPLLQERTLVSSA
                     DLPRYVGRVVMLAGWLIAERRVGLKGRGCMKFCTFEDLNGVFEAVLFPEIYQRYGHLL
                     DSHGPYFVTGRVQSGDDCCSLTVEKLERVGAMPKTPGASDITPPIHWIFPGVMEE"
     gene            complement(4565..6691)
                     /locus_tag="PKW60_01245"
     CDS             complement(4565..6691)
                     /locus_tag="PKW60_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPJ98086.1"
                     /translation="MSLKAYISALAAMGLVAGMAALSATTIEAAEGGGASVNETSRSA
                     ESLVEALFIPSSAAAGASPQVEVITLNSLTAPPSRPAEEAAYAAASAPALPAEAPMAL
                     VQAVPIRLEGMPMIRFAATEKAEEGEEAASNESAEAAPASPEPEPAPEAPVPPAPREA
                     RPEMKPVTREAKPIPVPVEGSSLAPAELKTAPAAQYQGDPLRKPVTIEFREMDLSNVV
                     ALLAQQADINVIAGTDITGTVTASLKNIPLMEAMEVVLGMNGLGIVKEGSIYRIVSID
                     EAIAAKRTTRFVVLEKAKPDEMKKTLDTIALSYPDGQSISVAANPSTSVVVLVGPDER
                     VQELEQIARQLDIAEPVIPTVTRAVKLNYAEPQGVRTVIEGILTKDIGVVEEDERGRH
                     IVITDTPLVVEQIEALIKELDTPVKQVAIDAMIVDAVMRDSSQTGVDWLMERIPGRNR
                     RGEVIGNLQELALDAKLGNIGSDLLDAGVLSIGTLSRDWNFRANLAAEVQSRNAEILA
                     NPVIVTVENKPAQIQIVQEYPYQELTQSTEGGSVASTEFKPIGVTLEVTPRVTHENDI
                     IVDVKTKQSSISGLSETGVPIEDKRQASTTLLTGNGRTIFIGGLRNVSDRLDISKLPV
                     LGDIPIAKFLFSQNDIEKVNTELLVFMTCNVVEKELPELSPEQRMRHDKLDTVPEVPD
                     SQRALFRSITHPEEVRDPAWKWRRKK"
     gene            complement(6696..7346)
                     /locus_tag="PKW60_01250"
     CDS             complement(6696..7346)
                     /locus_tag="PKW60_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98087.1"
                     /translation="MNKNTKKIVFLAILGVIAGYFVVTRVLSESPEDKQWREQYEANQ
                     KARVSGQAPAGPAGSGGAAAAAPASGPAAASTAKIDLEQLAAGIKEIDFNYDSVRKAE
                     QRRNPMAPLVGPYVAHPAMTASATGEPASTSDEAVVAAIRRNLLLSGIMWHPTAPVAI
                     INNEPIPTGHRFPNEMFRSARAAGGLDEAVTVTSMTQHSVILKYKESEITLELKER"
     gene            complement(7343..7918)
                     /gene="pilO"
                     /locus_tag="PKW60_01255"
     CDS             complement(7343..7918)
                     /gene="pilO"
                     /locus_tag="PKW60_01255"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HPJ98088.1"
                     /translation="MIGFLKGTVTPKDWMFVGAILGLTVLLCAAFFFLVYQPELKRHE
                     DTVKELADAQAELANRKELAANIDALRADTAAIRELVSDFEERLPSEREIATLLTQFE
                     DIAGDVGGLDVQMRAQMARREGAKLTIPYRIIVRGSFHDIAEFINRLEGFKRYLKVSE
                     LQVGPEKEGVSEAQFTLSTYTFVENDTEGAA"
     gene            complement(7915..8589)
                     /locus_tag="PKW60_01260"
     CDS             complement(7915..8589)
                     /locus_tag="PKW60_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98089.1"
                     /translation="MITINLLPKYLRPVKRTPIPHILSFLVLLAALAGMVVVFGAIHT
                     QLVLKRLELNGVQNELTALQSVKDEYDQLMADKTRLASKIKAIEEITRDRIIWSRQLY
                     NLSRLAPDNLWYTDIVVDAKRVEKEAPIIGKDGKPVIDPSTNKPRMGKINVDIPLLRV
                     SGFVIETPDGRLDTGTFARAAETDEEFSSLFRIEPPRVEDTTYEGYRVRKFTFEFVIK
                     KGGESS"
     gene            complement(8586..9839)
                     /gene="pilM"
                     /locus_tag="PKW60_01265"
     CDS             complement(8586..9839)
                     /gene="pilM"
                     /locus_tag="PKW60_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPJ98090.1"
                     /translation="MLFSKPSKAIGVDIGSHSVKAVQMTKVGGRLRIEQAGFGLVDRN
                     KINVDPIAAQAEAVRDALLRMNPPQSLLVGSIPGQNVVIRYPHLADMPENQIAQAVEN
                     EAGQNIPYELSEVYLDWSLLEKITDNGETTLRILLVAAKHEVIESRVQIANAAQIQYA
                     VLGVDSLALANAAESCDYLRLGESVALINLGASTTSIHFMKGGLSNFIRDISWGGREL
                     IQAIAKSRRCEFAEAEKMLSSASLLAPPSPGHEAAPEPAVSPLPATPEHTQSGSSPLD
                     PFDDELEGFGGRRAASSGIPARDAQGMGLGTGAAAPEKDLREVLTSPLARLVTEIRRS
                     FDYYEQQLYEKPVERLILSGGVAQLPGLPAALGEELGLDHVEVANPASSALILGQDAA
                     VAELRRRPAQFMVAVGLAARGAAEL"
     gene            complement(10313..10497)
                     /gene="ssrS"
                     /locus_tag="PKW60_01270"
     ncRNA           complement(10313..10497)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKW60_01270"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            10663..11976
                     /locus_tag="PKW60_01275"
     CDS             10663..11976
                     /locus_tag="PKW60_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98091.1"
                     /translation="MRKCYVMLFTALALGLAWAIRGHFGHEWGAAWAGGCGGLALLTG
                     MRDPRWMRRAPALAALCAIGWGAGGIMSYGIVIGYCRGNEFIDVMYGFSMLAVIGGLY
                     GFLGGGLFGLGLESTGDHKPQWAGLMAEMVAGAWLGWGLLIYQFEWLMTPPRSELWAA
                     CLGASLALAWYLYREGYARAMRVAAYSALGAGLGFALGNFFQVLGSSFDITYNWWNVM
                     EFTLGFCGGQGMAYAVATRTWPAPAAPSRLANRFALLFLLAAIPFVNHVKAVRTDALT
                     GLAESLKVIDVSQFVTRQQAWAWALAALLAAAGLLLWRRYGEHESTLTAAVAPGLFFG
                     YLTLYTLFSYITAGLFYKPVDFAVSHTAYVPILLLLFALWAIWGRRESHETVVERPES
                     ALRWLCIAACAGLFLVIIAQISIHMHDKPLGGAHQRFSAGTTAGG"
     gene            complement(12156..13613)
                     /locus_tag="PKW60_01280"
     CDS             complement(12156..13613)
                     /locus_tag="PKW60_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HPJ98092.1"
                     /translation="MNSIDLKYVVRLGIVLGVLLCLLPAAGSAAEPEAPIFRAGAARA
                     NITPPLGCDIVGNFEVPKATYIHDDLYARCLALDDGTVRLVIVLCDNLGTDQHVFDEA
                     KRRLQEETGIPPEHMLMAATHTHSGPSARISKCPGGYTEFTPYQQFMVDRIVDGVQCA
                     LKNLEPAEIAWGQGEEPGQVNNRRWYLSDPSLMISPFGAQDQVRMNPPRAHEALVEPA
                     GPTDPGIVFLAVRSVDGRPIALLANYSLHYVGGVPGEHISADYFGVFADRIQQLLGAD
                     RQEPPFVGMMSNGTSGDVNNINFRAPSPKRAPYEQMHRVANIVAAEVFRVYQTVEFKS
                     EAKLGGRMAQLTLPVRKPNEEQVQWAHETLAQPKDDVGVVKRSYAQRTLNLLECPDTV
                     DVWVQALRIGDAGIAGIPFETFAETGLRLKAEGPLPVTFTISQANGGFGYLPTPAQHA
                     LGGYETWLGTSKVAEDSEPVIVDTLFRQFAELAAE"
     gene            13722..14456
                     /locus_tag="PKW60_01285"
     CDS             13722..14456
                     /locus_tag="PKW60_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HPJ98093.1"
                     /translation="MDHPLKERFLERWNKYFPGAELPLVFYYTDHPREAEPPRELECL
                     FAQLADARQGRVLCFNKDSVVCPGGKRYLGFTQELKPKFEYFLSCGIPGTLEGERYKK
                     TPEIVKQLMKNAPPFTAPASYAVFKRWDRLEAGDEPEIVVFFAPPDVLSGLFTLAGYE
                     ESELDAVIAPFGAGCATIVQYPYLESRRDRPRAVLGLFDVSARPWVPRETLTLAVPMS
                     KFVQMVENMDESFLVTPSWDRVKNRM"
     gene            complement(14588..15715)
                     /locus_tag="PKW60_01290"
     CDS             complement(14588..15715)
                     /locus_tag="PKW60_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HPJ98094.1"
                     /translation="MEKTVDATPPPSKPMERIMSVDALRGFDMFWIVGADELFGALGK
                     ISHSPIAGFFARQLHHVSWEGFVFYDLIFPLFVFLVGMGIVFSMDKLIEREGMHGAHV
                     RIFRRFLLLFLLGAFRDMGIADMANESPFSGVLQRIAWCYLFTSLLYIHGKRRGLVIA
                     LVVILIGYWALLTFVPAPGMEAASYGKNENIVNWFDYKFLPFKEEGQYSDPEGLLSTI
                     PAVASCILGVFAGWLIRNRELPQEKKVIYLVAGGAALTVAGYLWGLQFPVIKRLWTSS
                     YVLVAGGYSAMLLGLFYLVVDIRQYRQWARPFIWIGANPLVIYMAGEFVRFHSMGERV
                     VGGPIAALFGAYGELLVVATSIVFVLLFARFLYNRKLFLRV"
BASE COUNT         2951 a         4741 c         4829 g         3345 t
ORIGIN      
        1 gccatggaac gccatattct tcacatcgat atggacgcat tttttgcttc ggtggagcag
       61 gtgctcaatc cggcactggt gggcaaaccg ctgatcgttg gcgggctgaa gagcgacagg
      121 cggggcgtcg taagcacggc ttcgtatgag gcgcgcaagt acggggttca ctcagccatg
      181 ccgctgattg aagcgaagcg gcggtgtcct cacggtattt tcatccgggg ccgcttcgag
      241 cattaccgga aagcctctga acagatccgc gcgatccttg agcgggtatc tccctgcgtt
      301 gagatggcat ccattgacga ggcctatgtg gatataacgg gctcccagcg ccttttcggc
      361 ggagatgatg ccattgcgcg ctacatcaag gccgccatcc gcgcggagac ccggctgccg
      421 tcgacgatcg ccatcgctcc gaacaaactt gtagccaaaa ttgcctcggg cgagggaaaa
      481 cccgacggct acctccggat aaatgcggag cgcgtgccca gcttccttcg tccgctcccg
      541 ttggcgaagc tgccgggtgc gggtccccga acgcgggaga tcctggaacg gctgggcatt
      601 atgacggtgg gaagcctcgc cgatgtgccg gtggacgtgc tggtacaagc cttcggctcc
      661 atggggtacg cacttcagcg cgcggcccgc ggagaatcca ctgcgccggt ccagccctgc
      721 tcacggccca aatccatcag ccgggagacg accttcgagg aagatctgct ggattgggag
      781 cgtatcgaac gaattgtggc ctatttggcg gaacgttgtg cgcacgcatt gcgcgaaaat
      841 ggtatggaga cccgctgtgt gacgctcaag gtgcggcata gcgacttttc aacgtatacg
      901 ttttccaaaa ccttgcccga gtccacctgt ctggagcggg atatcggcga ggctctcggt
      961 ggactgctgc ccaaagcccg ccagcgccgg gcccggctgc gcctggtggg cgtagccctg
     1021 acgtcgttga gttacgacca acgccagttg cggctgtttg acggcaaaga gccggagaaa
     1081 tggcttcggg tgatggaaag cgtcgatcgc attcgcgcac ggcatggttt cgaattcatc
     1141 cgtttcggaa aatcaatgga gctgggacgc catgttgagc tcgccacgcc gtcgctttca
     1201 cgatagagac ttcgaccctg aaaatgtccg gggcagggct cgcggaatgg gcagcgtttt
     1261 ttgaaggccc ccggggctgg ctccgatttt gatctccctg cagcacgggc agcccaggtg
     1321 gcgttgttca gcctgtccga aggctgcccc ggatctgcgc gaaagtgttt acggctttga
     1381 ttccgctgca tgtacacagc aactattcga tgctggaagg gacggcgccg gtggagcggc
     1441 tggtggagcg ggccgcgtcg ttcggattgc cggcgctggc gcttaccgat acgggcggac
     1501 tgtatggggc agtgccgttc taccggattg cgcgtgccgc gggcattaaa cccatcatcg
     1561 gcacctttct tgaaaacacc gttttgctgg ctcgtgatcg ggaaggttac gcccagctgt
     1621 gccgcctggt gagcgcgtac catctcgagg aaagcttcga cctgacggcc tgtttgagcg
     1681 aatgcagcgg ggtgttcgcg cttacggatg acgccgggcg cattcggcgg cttcaccgga
     1741 atggtctgga cccgctggtg gccttgactc atcatggcga cgcggtttcg cgttaccggg
     1801 cgacccggct gcgcgatttg gccgccgagc tcgggctgcg ttgtgtcgcg gcgaaccccg
     1861 tgtattttct gcagcctgaa gaataccgcc tgcaccgggt gttgtcagcc atccgctgca
     1921 acgccaccgt cgatacactg gaggcgggtt ctgccgcggg cgccgcctgc tggttccggc
     1981 cgccggaaga agttcgccgc ctttacgccg actggcctga cgcgctgcgc aacgcggaat
     2041 gggtggcgga ggaatgctgc ctcgaattgc cgctcggcac gccgctgttt cccgaattca
     2101 aactgcccgg cggcgaaacg ccgttttcat acctgtggaa actcgcgttt gacggggtga
     2161 aacagcggta catgccgctg aaccggcgca ttatcgagcg gctgcattat gagctggata
     2221 tcatcgagcg gctcggcttc gctccctatt tcattattgt gtgggatatc gtccatttcg
     2281 cccgcagccg cggcatacct gtcgtggggc ggggttccgc cgcgaacagc ctcgtggcct
     2341 atgcgctggg catcacccgg gtggatccgt tcaaatacga tctgtatttc gaacgctttc
     2401 tgaacccgtc ccggagcgat tgcccagata tcgatctcga catctgctgg cgccgccgcg
     2461 accaggtggt ggactatgtc tatcaacggt atggggctgg gcatgtggcc atgatcgcca
     2521 cgttcaacac cttccaggcc cgttcgtcag tccgggagat cgcaaaggcc atgggactca
     2581 ccggcagcga gatcggggag gtgacgcgcc gcctgccccg gtatcatgcg ggcgacattc
     2641 agaccgtggt gcgctggctg cccgagtgcc gtgaactgcc catccacgaa gaaccgttgc
     2701 gatccattgt ggcaatcgcc gaggcgattg atcagtatcc acgccatatt tcactgcatc
     2761 cgtgcgggct ggtgatcgcg cccgaaccgc tcacacgctt cgtgccgctt caacggaccg
     2821 cgaaagggcc ggtggtcacg cagtatgaca tgcaccccat cgaggagctc ggcctcatca
     2881 agatagacct tttggggcat cgctcactga cggtgattga cgagacggtt caacaggtgt
     2941 gggataaccg gggcatccgg ctcgatgttg aaacgcttcc agagcctgat ccgtgcaccg
     3001 cggatctgat tcgcaacgga cgtaccatcg gctgctttca gatcgagtcg cccgccatgc
     3061 gcacactgct gcgcaacacg caggccgccg ataccgacat gctcatcaag accctgtcac
     3121 tggtccgtcc ggggccttcg ggcagcggca tgaaaaagcg cttcatcgcg cgccacctgg
     3181 gcaaggagcc ggtcgagtat ctgcaccccg cgctcgaacg cgtgctcagc gatacctgcg
     3241 gcgtgatgct ctaccaggaa gacattctca aagtggcgaa tgttattgcg gggatggatc
     3301 tcaccgaagg cgatatgctg cgccgtgcca tgaccaaaaa gcggtcgccc cgggaaatgg
     3361 cgaagagcat gaagggcttc atcgaaaagg ccgtcgcgaa tgggattgca gaggatgttg
     3421 ccggagaaat ctgggggctc atcgccaatt ttgccgagta tgcatactgc aaagcgcatg
     3481 cggcaaccta tggcgagatc gcctatcaat gcacgtatct gaaggcccat ttcccagccg
     3541 aatttatggc ctgtgtgctg tcaaaccggg gtggtttcta ccatcccgcg gtttatgtgg
     3601 aagaggcccg gcggctgggc atcgaggtgc gcccgcccga catcaacaaa agccgcttcg
     3661 aatacgccgc cgaaggccgc gctatccgga tggggtttgt agaagtgcgg caactggctc
     3721 aaaaaacagt cgacgccatt cttgccgggc gcgcaaagcg tccgtttgaa agtctcgcgg
     3781 acgtgtggag ccgcaccggt atgagcaggc ccgaagcgga agcattgatc caggccggcg
     3841 cgttcgatgc attcggccgg acgcggcctg cgctgttttg ggagctgcat cttttggcac
     3901 agggcggaga gagggaagag cctgccggcg cggcccgttt gttccgcgag catgggggag
     3961 agtaccgcac cgtgccccgg ctgcctgacg tgtcgcggaa aaaacgcacc ggccaggaat
     4021 gggaggcgct gggactcatg gtgtcgaccc atccgctcga gtactatgcg ccgctgctgc
     4081 aggaacggac cctcgtgagc agtgcggatc tgccgcgcta cgtgggccgc gtcgtgatgc
     4141 tggccggctg gctgatcgcg gagcggcgtg tggggctcaa agggcgtggc tgcatgaaat
     4201 tctgcacgtt cgaagacctc aacggtgtct ttgaggcagt gttatttccc gaaatatacc
     4261 agcgttacgg ccacctgctc gattctcatg ggccgtattt tgtcacgggc agagttcaga
     4321 gcggcgacga ttgctgctcg ctcaccgttg agaaactcga acgggtgggc gcgatgccca
     4381 agacacccgg ggcttccgac attactccgc ccatacattg gattttcccg ggcgtgatgg
     4441 aggaataaca gcggaaagcg tcgtattcgg gcaggcttgg gaaaacaggc tggaaaaccg
     4501 gccgcgccgg ctaaaaaaaa ggccgcaagc ggtgaggctt gcggccgtgg ggatgcggga
     4561 acggttattt cttgcggcgc catttccagg ccgggtcacg gacttcttct ggatgcgtga
     4621 tgctgcggaa cagcgcgcgc tgagaatccg gcacttcagg aaccgtgtcg agtttgtcgt
     4681 ggcgcatgcg ctgctcgggg ctgagttccg gcagctcctt ctcaacgacg ttgcaggtca
     4741 tgaacaccag cagttcggtg ttgaccttct cgatgtcatt ctgactgaag aggaacttgg
     4801 cgatcgggat gtcgcccagc accggcagct tgctgatgtc aaggcgatcg gagacattgc
     4861 gcagcccgcc gatgaagatg gtgcgtccat tgccggtgag aagcgtcgta gaagcctggc
     4921 gcttgtcttc gatgggcacg ccggtctcgg agagtccgct gatggagctc tgtttggtct
     4981 tcacatccac gatgatgtcg ttctcgtggg tcacgcgcgg tgtgacctcg agggtcacac
     5041 cgatgggttt gaactcggtg gacgccacgg aaccgccttc ggtggactgc gtcagctcct
     5101 ggtagggata ttcctggacg atctgaatct gggcgggctt gttttccacc gtgacgataa
     5161 ccggattcgc cagaatttcc gcattgcggc tctgaacctc cgcggcgaga ttcgcgcgga
     5221 agttccagtc gcggctgagc gttccaatgg acagcacgcc ggcgtccagc agatcgctgc
     5281 cgatattccc cagcttggcg tcgagggcga gttcctgcag attcccaatg acctcaccgc
     5341 ggcggttgcg gcccggtatc cgttccatga gccaatcgac gcccgtctgg gacgagtcac
     5401 gcataacggc atcgacgatc atcgcgtcga tcgctacctg tttcaccggg gtgtcgagtt
     5461 ctttaatcag cgcttcaatc tgctcgacca cgagcggggt atcggtgatg acaatgtggc
     5521 ggccgcgctc atcttcctcg accacgccga tatccttggt aagtatgcct tcaatgacag
     5581 tgcggacgcc ctggggctcg gcatagttga gcttcaccgc gcgcgtcacg gtcgggatga
     5641 ccggttcggc gatgtcgagc tggcgtgcga tctgttcaag ttcctgcacc cgctcatcgg
     5701 gtccgaccag gacaacgacg ctggtgctgg ggttggcagc gacggaaatc gactgcccgt
     5761 cagggtaact caacgcgatc gtgtccagcg tctttttcat ctcgtcgggt ttggcttttt
     5821 caagcaccac aaagcgagtc gtgcgtttcg ctgcgatcgc ttcgtcgatg ctgacgatgc
     5881 ggtagatgga accttccttc acaatgccca ggccgttcat gcccaggaca acctccatgg
     5941 cctccatgag cgggatgttc ttgaggctgg ccgtcacggt gccggtgatg tccgtgcccg
     6001 caatgacgtt gatatcggcc tgctgcgcga ggagggccac aacgttgctg aggtccattt
     6061 cgcggaattc gatggtgacg ggtttgcgca acggatcgcc ctggtactgc gccgcgggcg
     6121 cggtcttcaa ttcagcgggg gcaaggctgc tgccttccac aggcaccgga atcggcttgg
     6181 cctcccgcgt gaccggtttc atctccggcc gggcttcccg cggggccgga ggaaccggcg
     6241 cctccggcgc gggttccggt tcaggcgagg ccggggcggc ttcggcgctc tcgttcgacg
     6301 cggcttcctc gccctcctcg gccttctctg tggcggcgaa acggatcatg ggcatgcctt
     6361 cgagccggat cggcacggcc tgcaccagcg ccatcggcgc ttccgccggc agggctggcg
     6421 cggacgcggc tgcgtacgcc gcctcttccg cgggccggga aggcggcgcg gtcaggctgt
     6481 tcagcgtgat cacctcgacc tgaggcgacg cgccggctgc cgccgaggac ggtatgaaca
     6541 gggcttccac caggctttcg gctgaacggg aggtttcgtt cacgctggcg cctccgcctt
     6601 ccgcggcttc gatggtggtc gccgacaggg cggccattcc ggctacaaga cccatcgcgg
     6661 ccaacgcaga gatgtaagct tttagcgaca ttaccttacc gctccttcag ctcaagggtg
     6721 atttccgact ctttgtattt cagaatcacg ctgtgttgcg tcatgctcgt gactgtaacc
     6781 gcctcatcca ggccgcccgc cgcgcgcgcg gagcgaaaca tctcgttcgg gaagcggtgg
     6841 cctgttggaa ttggctcgtt gttgatgatc gcgaccggcg cggtggggtg ccacatgatg
     6901 cctgacagca gtagattccg gcgaatcgcc gcaacgacgg cttcgtcgct cgtgctggcc
     6961 ggctctcccg ttgccgaggc tgtcattgcg ggatgtgcga cgtagggccc cacaagcggc
     7021 gccatcgggt tacggcgctg ttcggccttt cgaaccgaat cgtagttgaa gtcgatttcc
     7081 ttgattccgg ccgcgagctg ctcgagatcg atctttgccg tgctggcagc tgctgggccg
     7141 ctggccgggg ccgccgccgc ggcgccgccg gaacccgccg gaccggcagg ggcctggccg
     7201 ctgacgcgcg ctttctggtt cgcctcgtac tgctcacgcc actgcttgtc ctcgggagac
     7261 tcgcttagca cgcgggtcac cacgaaatat ccggcaatca cgccgaggat agcgaggaaa
     7321 acaatctttt tggtgttttt gttcacgccg cgccctccgt atcgttttct acgaaggtat
     7381 aggtgctcag ggtaaactgc gcctccgaca ccccttcttt ttcgggcccg acctggagtt
     7441 cggagacttt aaggtaccgc ttgaaacctt ccaggcggtt gatgaactcg gcgatatcgt
     7501 gaaaactccc gcgcacgatg atgcggtagg gaatggtcag cttggcgcct tcccggcgag
     7561 ccatctgggc gcgcatctgc acatcgaggc cacctacgtc gccggcgata tcttcaaact
     7621 gcgtgaggag cgtggcgatc tcccgttcgg aaggcagacg ctcctcgaaa tcgctcacca
     7681 gttcccggat ggcggcggtg tccgcacgca gcgcgtcgat gtttgcggcg agttcttttc
     7741 ggttcgcgag ttcggcctgc gcgtcagcca gctctttcac cgtgtcttcg tggcgcttaa
     7801 gctccggctg gtacaccagg aaaaagaagg cggcgcacag caggacggtc agcccaagga
     7861 tggcgccgac aaacatccag tcttttggag tcacggtgcc tttcaaaaag ccgatcatga
     7921 tgattcgcct cccttcttga tgacgaattc gaaggtgaac ttgcgaaccc tgtagccttc
     7981 gtacgtcgta tcctcgacgc gcgggggctc aatccggaac aaagacgaaa actcttcgtc
     8041 ggtttccgcg gcccgggcga aggtgcccgt atccaggcgg ccgtcgggtg tttcaatcac
     8101 aaaaccggac acacggagca gcgggatgtc gacgttgatt ttgcccatgc gcggcttgtt
     8161 ggtgctggga tcgatcaccg gcttgccatc cttgccaatt atgggcgcct ctttttccac
     8221 ccgtttggcg tcgacgacaa tatcggtata ccagaggttg tccggcgcca ggcggctgag
     8281 gttatacaac tgccgcgacc agatgatgcg gtcgcgggtg atctcctcaa tggctttgat
     8341 cttgctcgcc agccttgttt tgtccgccat cagctggtcg tattcgtctt tgacggactg
     8401 cagggccgtg agttcgttct ggacgccgtt gagctccagg cgcttgagca cgagctgggt
     8461 gtggatagcg ccaaacacga ccaccatgcc cgcgagggca gcgagcagga ccagaaagct
     8521 caggatgtga gggatggggg tccgctttac gggccgcagg tatttcggca gcaggttgat
     8581 cgtaatcata gttccgcggc tcctcgcgcg gccaggccga ccgcgaccat gaattgagcg
     8641 ggccgccgcc gcagctcagc taccgccgcg tcctggccca gaatcagggc gcttgaggcc
     8701 gggttggcca cttcgacgtg gtcgagaccc agctcttcgc caagggctgc cggcaggccc
     8761 ggcagctgag ccacgccgcc gctcaaaatc agccgttcca ccggtttttc atacaattgc
     8821 tgctcgtagt aatcgaatga gcggcggatc tccgtgacca gccgggccaa cggcgaggtc
     8881 aacacctccc gcaggtcttt ctccggcgcc gcggcgcctg tccccaatcc catcccttga
     8941 gcgtcgcggg ccgggatgcc cgaagaggcc gcgcggcggc cgccgaaacc ttccagttcg
     9001 tcgtcaaagg gatcaagggg cgagctgccg ctctgcgtgt gctcaggcgt tgccgggagc
     9061 gggctgaccg cgggctccgg cgcggcttcg tgccccgggg acgggggcgc caggaggctg
     9121 gcgctggaca gcattttttc cgcttccgca aattcgcagc gccggctttt ggcaatggcc
     9181 tgaatcaact cgcgcccgcc ccagctgatg tcgcgaatga agttggagag accgcctttc
     9241 atgaaatgga tgctggtggt cgatgcgccc aggttgatga gcgccaccga ttcacccagg
     9301 cgcaggtaat cgcacgattc cgcggcgttg gccagggcca gcgagtccac gcccagcacg
     9361 gcatactgga tctgtgccgc attggcgatc tgaacccgcg attcaatcac ttcgtgtttt
     9421 gccgcaacca gcaggatccg aagggtggtc tctccgttgt cggtgatctt ctcaaggagc
     9481 gaccagtcca gatagacttc cgacagttcg tacggaatgt tctgtccagc ctcgttttcc
     9541 actgcctgcg cgatctggtt ttcgggcata tccgccagat gggggtagcg gataaccaca
     9601 ttctgccccg ggatagagcc tacgagaaga ctttgaggcg ggttcatgcg caacaacgcg
     9661 tcgcgtacgg cctcggcttg tgctgcgatt gggtcgacat tgatcttatt gcgatcgacc
     9721 aggccaaatc ctgcctgctc gatgcgcaac cgccccccaa ctttcgtcat ttgaaccgct
     9781 ttgacggagt gggagccgat gtctacgcca atggctttac tcggcttgga aaacagcatt
     9841 ggcctcaatt ccccgttttc gaggcgtttg tgtgcccatt gccgcggcaa gctcccctga
     9901 acctggcaag tggtgtgctg ggcacacagc gacccgtatt ccgcttcttc ctacactaga
     9961 tactaccaaa aatgcgaaac tttatcaacc ctaaactttt tggaaagcga ttcccctggg
    10021 agggtaatat caaggaaaac gccctcaaac cagcgtgatc ctgctgtgca aaggtcatgc
    10081 cacgcacggc ctgccaggca aaaacgccct gaaatggcgt ttttttgaag attatctctt
    10141 gactgattgc ggatgggagg ggcggggcag tgccggaatc ctgtcggagc ggataaatcc
    10201 tgccgattcg gcagccctga agcccgccca aaaaaaaccc aatgtgaaat tggcgcgaag
    10261 cagtcagccc ggcgcgcaca aaaaaagcgg gattcatgga atcccgcttc ttgacttccc
    10321 cgcgctgtgc cgtagacgag acgaaaaatt gaacctcgcc cactaagggg gtgccctcac
    10381 ggcagcctcc cggcttcccc ggcgaggggg catgccgtca gctgctcaaa tttcgggctc
    10441 ccggttcaat tagtaaggta caattttcgc cctgtccctc gaacacggca gggaaactct
    10501 gaatactgta aactgtcgcc ggtcccgccg ttgccggaca tattcttaca tgattttcac
    10561 gccagcggcg tagaccatct tgcctttccc aagtgaaagc attatacctt aatttcaagg
    10621 tcggtgggca gttttttgcg cggatgcgta ccggagatgg ccatgcgtaa atgctatgta
    10681 atgctcttca ctgccctggc gctgggtctg gcgtgggcga tccgtggaca tttcgggcat
    10741 gaatggggtg ccgcatgggc tggaggctgc ggagggcttg cgctcctgac cggcatgcgc
    10801 gacccgcgct ggatgcgccg cgcgcctgcg ctggcggcac tctgtgctat cggctggggc
    10861 gcgggcggga tcatgagtta tgggattgtc atcgggtact gccggggcaa tgaattcatt
    10921 gatgtaatgt acggcttttc aatgctggcc gttatcggtg ggctctacgg ttttctcggc
    10981 ggaggcctgt ttgggctcgg gctcgaatcg accggtgacc ataagcctca gtgggccggg
    11041 ttgatggctg aaatggtcgc cggagcatgg cttggctggg gcctcctcat ctatcaattc
    11101 gaatggctta tgactccgcc gcgttcggaa ctatgggcgg cgtgtctcgg cgcctcgctc
    11161 gcgctggcct ggtatctgta ccgcgaagga tacgcccggg ccatgcgggt ggcggcctac
    11221 agcgccctgg gcgctgggct gggctttgct ctgggcaatt tttttcaggt gctgggctcg
    11281 tctttcgaca taacgtacaa ctggtggaac gtgatggagt tcacgctggg gttctgtggc
    11341 gggcagggaa tggcctatgc cgtggctacc cggacatggc ccgcgcccgc agcgccgtca
    11401 cggctggcca accggtttgc cctgctgttt ctcttggcgg ccattccctt cgtgaaccat
    11461 gtcaaggcgg tgcgcaccga cgcattgacc ggcttggcgg aatcgcttaa agttattgat
    11521 gtatcacagt ttgtgacccg gcaacaggcc tgggcctggg cgctcgcggc tcttctggct
    11581 gccgcaggac tccttttgtg gcggcggtat ggggagcacg aatcaacact gaccgccgcc
    11641 gtggcgccgg gcctgttttt tgggtatctg actctgtaca ccctgttttc gtatatcacc
    11701 gcaggtcttt tttacaaacc agtcgatttt gctgtctccc ataccgcata cgtgccgatc
    11761 cttctgctgc tctttgcctt atgggcgatc tggggtcgga gagaatcgca tgagacagtt
    11821 gtggaacgcc cggagagtgc gttgcggtgg ctctgtattg ccgcatgtgc cgggctgttt
    11881 ctcgtgatca tcgcccagat ttcaattcat atgcacgata agcctctggg tggcgcccac
    11941 cagcgcttct ccgccggaac aactgccggc ggctgaaaag catggagaag cactccgcac
    12001 tgtcgcgcaa tttgttgcct tagcagacaa ggtttgcctg aggttaaggc cttgccatct
    12061 tctccccgct gggaagggtg cgctgtttgg gcgtagtgcg ggcttagtgt gcttaatacc
    12121 cgagcgcaat attgctgtct tttgcgccgc ccccgttact ccgcggcaag ctccgcgaac
    12181 tggcggaaaa gggtatcgac aattaccggt tccgaatctt ccgcaacctt gctggttccc
    12241 agccaggtct cgtatccgcc cagtgcatgc tgggcaggcg ttggcaggta tccgaaaccg
    12301 ccattggcct gcgaaatcgt gaaggtcacg ggcagcgggc cttccgcttt aaggcgcagt
    12361 cctgtttctg caaaggtctc aaatggtatc cccgcgatcc ccgcgtcgcc gatgcgcaag
    12421 gcctgcaccc agacgtcgac ggtatccggg cactccagga gattcaaggt acgctgcgcg
    12481 tacgatcgtt tgacaacacc cacgtcgtcc ttgggttggg cgagggtctc gtgagcccat
    12541 tgcacctgtt cttcatttgg cttgcgcacg ggaagggtca actgggccat gcggcctccg
    12601 agctttgcct cgctcttgaa ttcaacggtc tggtataccc ggaagacttc cgctgccaca
    12661 atgtttgcca cgcggtgcat ttgttcatag ggtgcccgtt tgggcgacgg cgcgcggaaa
    12721 ttgatgttat tgacgtctcc acttgttccg ttggacatca tccccacaaa cgggggctcc
    12781 tggcggtccg cacccagcaa ttgctggatg cgatccgcga acactccgaa atagtccgcg
    12841 gaaatatgct ctcccggcac tccgcccacg taatgcagcg aataattcgc cagtaacgca
    12901 atcggacgcc catccacgga ccgcacggcg agaaatacga ttcccggatc ggttgggccg
    12961 gccggttcca ccagcgcctc gtgcgcccgg ggcgggttca tgcgcacctg gtcctgcgct
    13021 ccaaaagggc tgatcatcaa cgaagggtcg ctcagatacc atctgcggtt gttgacctgg
    13081 cccggttctt cgccctgtcc ccaggcaatc tcggcgggtt cgaggttctt gagcgcacac
    13141 tgaacaccat cgacgatgcg gtcgaccatg aattgctggt atggagtgaa ttccgtgtat
    13201 ccgccagggc attttgaaat ccgcgcggac ggccccgaat gcgtgtgcgt cgccgccatc
    13261 aacatgtgct ccggaggaat cccggtctct tcctgaagcc gccttttcgc ttcgtcaaag
    13321 acgtgttgat cagtgccgag gttatcgcac agcacaatca cgaggcgcac cgtgccgtca
    13381 tccagcgcga gacagcgcgc gtacaaatcg tcatgaatgt atgtggcttt tggcacctcg
    13441 aaattgccca cgatatcgca acccagaggc ggagtgatgt ttgcccgggc cgcacctgca
    13501 cggaatatcg gcgcctccgg ttccgccgca ctgcccgccg ccggcagcag gcacagcagc
    13561 accccaagca caatgcccaa acgtacaaca tacttcaggt ctattgagtt cattggctgt
    13621 ctccctgagt tatggtatct tcgcattcta tcagatgtgc gcgtaaagga cacgcgccgc
    13681 gccacctgcg gccgaacagc cgcaccagga gagtgcctgc catggatcat ccgttaaaag
    13741 agcggtttct tgaacgctgg aacaaatatt tcccgggcgc ggagctgccg ctcgtcttct
    13801 actatacaga ccaccctcga gaagccgagc cgccccgcga actcgagtgc ctgttcgccc
    13861 aacttgcgga tgcccggcag ggccgcgtcc tctgcttcaa taaggactcc gtcgtgtgcc
    13921 cgggcggcaa gcggtatctc gggtttacac aggaactcaa gcccaaattc gagtattttc
    13981 tctcttgcgg catccccggc accctggaag gcgaacgata caaaaaaacg ccggagatcg
    14041 tgaaacaact gatgaaaaat gcgccgccat tcactgcacc ggcctcgtac gccgtcttca
    14101 aacgctggga tcgcctcgaa gccggtgatg aacccgagat cgtcgtgttt tttgctccgc
    14161 ccgacgtgct ctccggcctg tttacgcttg ccggctatga ggaatcggaa cttgatgcgg
    14221 tcattgcgcc atttggcgcc ggttgcgcca ccatcgtgca gtatccgtat ctcgagagcc
    14281 ggcgcgaccg gccccgggcc gtcctgggtc tgtttgatgt ctccgcacgg ccctgggtgc
    14341 cgcgcgagac tctcacgctt gccgttccga tgagcaagtt cgtgcaaatg gtcgaaaata
    14401 tggacgaaag ctttctcgtc actccctcat gggatcgcgt caaaaaccgg atgtaacact
    14461 gaagtcccag gcaacgtgaa agcgttgcct gggactctca tcgccaaccc ggaaaatgtc
    14521 ttcaagcggg ggacatgctt gcgcacctca aagggcgcca gcgccgctca gacctgtcag
    14581 gcaggcgcta cacgcgcaaa aacagcttgc ggttgtacag gaatcgcgca aacagcagca
    14641 cgaagacgat gctggtcgcg acgaccagca gctcgccgta agcgccgaac agtgctgcga
    14701 tgggccctcc tacgactcgt tcacccatgc tgtggaagcg gacaaactcg cccgccatgt
    14761 agatgaccag ggggttggcg ccgatccaaa tgaacggacg cgcccactgc cggtattgcc
    14821 ggatatccac cacgagataa aacaagccca gcagcatcgc gctgtatccc ccggcaacaa
    14881 gcacatacga agaggtccac agccgcttaa tcacagggaa ttgcaggccc cagaggtagc
    14941 ccgcaacggt taatgctgct cctcccgcca ccagataaat gacttttttc tcctgtggca
    15001 attccctgtt gcgaatcagc catcccgcaa ataccccgag gatgcacgag gcgaccgcgg
    15061 ggatcgtgct caggagtcct tccggatccg agtactgccc ttcttcctta aacggaagaa
    15121 acttgtagtc gaaccagttg acgatatttt cgtttttgcc gtaactggcg gcctccatgc
    15181 ccggcgccgg gacaaacgtc aacagcgccc aatatccgat caggatcacg accagcgcaa
    15241 tgaccagccc tctacgcttc ccgtggatat acagcagcga cgtgaacagg tagcaccagg
    15301 cgatgcgctg cagcaccccg ctgaacgggc tctcgttggc catgtctgcg atgcccatat
    15361 cgcgaaatgc tcccagcaga aacagcagca ggaaacggcg aaaaatccgg acgtgcgcgc
    15421 cgtgcatgcc ctcgcgttcg atcagcttgt ccatgctgaa tacaatgccc atccccacca
    15481 gaaagacgaa caagggaaag atcagatcat aaaacacgaa tccttcccag ctgacatgat
    15541 gcagttgacg cgcaaaaaaa cctgctatgg gcgaatgact gattttgccc agcgcgccaa
    15601 acagctcgtc ggcgccgacg atccagaaca tgtcaaaccc gcgcaaagcg tccaccgaca
    15661 tgatgcgttc cattggtttg gacgggggag gcgtagcgtc cactgtcttt tccataggtg
    15721 gtgctctcct tttttgcgcc cccgtctgcc cgccgacagc cggggcacgc tccgaattcg
    15781 cggccacata agggttttgc cgattcctct agactatcag aaacatggca tttttgaaca
    15841 tattgttctc tcgtcttctc ttgtct
//
LOCUS       DAOPBQ010000017        15748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_3798_length_15748_cov_4.143758, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000017 DAOPBQ010000000
VERSION     DAOPBQ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 15748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15748
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3798_length_15748_cov_4.143758"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="PKW60_01295"
     CDS             complement(<1..1668)
                     /locus_tag="PKW60_01295"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HPJ98095.1"
                     /translation="MAAPQEDSGIQFKVRDSKTGEDVKLAMEELWLTGKVLPVGARLV
                     LRHVFKSAEIKPLELVYTYGLPRDATLRSFRVVGEGFTVESELKLTEKAREEYEEGIA
                     QGHLAVLNRVYNDGLASLSVGNVRPGETVAVYLEILAGVEHHDDGFRFRFPFTLAPSY
                     HRKARVAVTPEGYGEMELPEDRYDDLLLPPWSSDAQNLHTVGFDLLVEFPGRGAQVAS
                     PSHLLAARMGDEDSAYVQLANMDDLPNRDLVLDVKMEASEPKVFAGIDKDSQTQFIGV
                     VPSTAFGKRPDTPMRVAFVVDRSGSMGGAPMEQAKKAIRACLGALRPEDQFGIVAFDD
                     RTSVFAADLVPGTRENREKAAEFLSRVDARGGTELLEGLSAATRLFGEPGGDIFLLTD
                     GEVFAGSEIMAGMHDRGFRIHCLGIGSASQDRFLALLARETGGVSRFMTPRERVDLAA
                     LELFASVGRPVATGVGCIVAGSTDARLSPAPADTVFAGTPLVVYGSCPADAECVLQLK
                     FEGADGSGTLEYPIAAAPSDLAETLRLLRGARLITDLESQLSQAGTES"
     gene            complement(1683..2090)
                     /locus_tag="PKW60_01300"
     CDS             complement(1683..2090)
                     /locus_tag="PKW60_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPJ98096.1"
                     /translation="MGAENRDHPQEMKLNELADRAGVPARTIRLYITQGLLPGPLRAG
                     RNAAYGAEHLDALAAIKEFQRQGLTLSQIRRKMAVAEEVEQPLAAPVQTWVYQVAPDV
                     SVTIRGDTPPWRLRVIQKALVELQRALNVRDDA"
     gene            complement(2356..3591)
                     /locus_tag="PKW60_01305"
     CDS             complement(2356..3591)
                     /locus_tag="PKW60_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98097.1"
                     /translation="MKPRPDLLTKDAPTNAEVFQLTDEAIPSCHVYMEAQIFTPDSKR
                     FVLHRSAHPHGSNKSDPEHRYLLCDLENGGELSPLTDELGVTAPSVSPDGTYMYYFIN
                     ETSLNAGQLTLKRVRLDGSDRETLMVLDAPPPGAACIPSHIYPLSTISSDGKRLALSA
                     FFGDGDTEDAPFGLMVFDLEKGAVRVVLAGATWLNMHPQYCRSTAPEAAHDILIQENH
                     GSEYTKDGNCTRLVGGDGADIHLIRDDGSDFRSLPWGRDGNEFCQGHQCWLGDSTLAI
                     TSTSVRDSRHCELILSPPASFAGHIGISTPGGQRNNLSRVFPKPAFYHFATDLAGKRL
                     ITDARIGNTWLLFTAEFGPAPDDPLTNWQFVLDSQTDAIKKTAHIHPFLSRDGTTGFF
                     NSEESGVLQAYMVTGLFPT"
     gene            complement(4097..8404)
                     /locus_tag="PKW60_01310"
     CDS             complement(4097..8404)
                     /locus_tag="PKW60_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HPJ98098.1"
                     /translation="MSAYRGRDIPWLSVVRFHKEVVTRAEQGFFSLSGRDDQAERWTS
                     LPNFEPSDLAGPWQIDCDGIRSLPFRLAFEQGQHESLFLGGPCFLGWSKSMREEWMPQ
                     WRPLLYREVELRPNGSRFEIVPREGSWSVTPLIYSLLDQLETSVGDSPDEFAASLIEK
                     AAGYRQLEQAPLDRCIFRAVFSEVPETEAEFTKKIRRDTFDVQPTPWVLFAPTRDFSA
                     FTRHLMQDYERLEALLTRNPDQIGGLRLLEDQPTTLPVTQTEVLPLVPLNDSQRMAVQ
                     RILEEHPLTVISGPPGTGKSQVVVSLLLNAWARGQTVLFASNNNKAVDVVRERVERFE
                     SEFPIAVRAGARHKQNIQEVLRRTLNMAAVTNGEDALGVDVERLRRQRIHLLDRKREL
                     QSELETKLPQRIDESRKTALRAYGEYRKTLASIADQNHALEVEKEKLGFEDKCFTKIE
                     SSVEDTAGWLQRMQLFKDLIARDNQRRQKLLAEIADRERHRDRAIEEVGLSVAEAGDW
                     RWLLTGPSPEILADFDQRLRVILDKPVEQALEPIEWREEFDRWRSGEQANGWATEARE
                     FAEVVLRSCAKLAPKLGKIRQLSAAVGEGRSKVSAIGMPTDVEIPLEHLRGWKADFAE
                     YVTHEGRWFDFLPWSELSRLRRSLRRHEKQIRSGLPLEFWTRIGTLNDAGRAQIAPII
                     EVIGRWIELRDAWQSAQTLIGEIEDRFQELRSQAAALRLRDVPSSPSIEAWAHIVTQC
                     ETTASLADAAARAWKRRTEKEETQEQLRTIAKEWSRLASGVPIREAWRRGQGVNFDGA
                     LRDLADAPGQETVNAARAAVYTGSLTHLVECWQAASDHEQAASQLRADFKAVPEPVEH
                     VKAWWRERPNSAFVLAGQSLNWPDIEEAREQIEVVSALCDRWRAFDKTTRPSMEAKAQ
                     QELEWAVAKLGQAVEALPEGSERIQVTKVFDLIKKEPESDWPVYDLNDAFAEFSPDRI
                     RAKIGRIEVELEKGSFEDAKAKWLERIRGDDDAIRAVDRLERALRRHRGVFVESEYQT
                     FRAALRAVPIWITTAQASQAIPLASELFDIVIIDEASQCTLTNLLPLMYRARTLTVIG
                     DEHQLPAIPTLQESEELALARKYGIEEHLSLVGHATNDVYKTATASLPRRRSDVLMLN
                     EHFRSNPQIIGFSNRYIYLQRLELKKDPSWGARLPIGSGVHVFPVTGLAKRGPNDRSW
                     VNVPEAEEVLKRVQQLKQGDSRSLSLGVVTPFAAQKETLRERLDKLQLSSEVLVDTAY
                     GFQGDERDVIIFSPVVAKGITVSASRWVESPPNLINVAITRAREAVFVVGDIDYCLQQ
                     DGFLRKLALYCRDIQLLRDTSPAELELFSWMVVKGWEPNIHPNVGDTEVDFILKSASG
                     LRLVIEVDGRSHLQMIEQDRARTAYLQGQGYEVLRIPAREVFETPFEVIHRIEQSLA"
     gene            complement(9055..11145)
                     /locus_tag="PKW60_01315"
     CDS             complement(9055..11145)
                     /locus_tag="PKW60_01315"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ98099.1"
                     /translation="MSDKAAEHNYRMALMFYRTERYEDAMAILDDLALEFPDSKHVLY
                     ALAKCFAKVGRYQEARALCQELAERHQDERAATLLERIGDRPDSYIPQNTFQEAPPEL
                     PPDIGVREEGAAEDSSTQAATPASDTTSSWMSSTPAGLMYDSSPEKESSYSSAGSYST
                     PLSDAVTPFITPPPLSEFEEKPVPGPSTPPALETTPVEEPAKKPFISNGMIAVIALAL
                     AGAVLILWVAFGPRQFVFPDDHTMGTLVIREIDGSHVRWRDYGAAQGQTSFPKWEYIG
                     LNASPELTEADLVLLQKASWLKQLDLSKSNVTDEMIDALAELRQLRVLDVRDTQLSEE
                     GIARLRKWLPDCTLRFDVASAPKPAAAAPAPVPVATAAPKPSEPPAPAPAPKAPTPKP
                     PEPVTPATPPGPRELIFADMVGDVQVKAWDALDSTDWKSFQPAKGRVVVPAERIVKLV
                     VKAGSAQLRAIQLLSPDDLHSLDVSAARLEDDAAPIVARFTGLRSLLADDAQITNKGL
                     TQLCALTGLRELSLAGADKYDAAGMAKFSALTSLERLTLRGGNVNDECLQHLAGLTAL
                     RRLDLSRTAVSDAGLASLAKLSQLTELNLSHTQVSGPGLVHLQALRSLQRLDLAGNSI
                     NEEGLQHLAQCRHLQFIQMDGPDITDASILVLAQIPTLRQVNLANTGVSAEGAAALKK
                     AMPGCSVLLSKSRQ"
     gene            complement(11339..11791)
                     /gene="fliS"
                     /locus_tag="PKW60_01320"
     CDS             complement(11339..11791)
                     /gene="fliS"
                     /locus_tag="PKW60_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HPJ98100.1"
                     /translation="MGDAQTTAQLGAYKRVDVETTSQGKLIVLLFNGAIQRAEEAKRH
                     IAAGRIGNVHNNLIRAQEILGELRSALDMKTGAIAQNLDRIYEYLQHLLITANLRKEI
                     EPIDECLSHLTTLRDTWKEAFEKLAAEGQPVNPPAMHQHGASLMNLRG"
     gene            complement(11829..13547)
                     /gene="fliD"
                     /locus_tag="PKW60_01325"
     CDS             complement(11829..13547)
                     /gene="fliD"
                     /locus_tag="PKW60_01325"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HPJ98101.1"
                     /translation="MSGAFSAGGLITGLDTNALIAQLIALERRPIARLQQRIAALETQ
                     RGAIGGVRTQLQTLRNRAEDFRLNTVFGQFRTASSENTVLATSLTGANAAPGVYTVEV
                     LQLASATIARSSSALGAAISPDAALSGSGIHTEVTAGSFTINGTTFTIDPDTQSLNDI
                     LAEINSSGIGVQAHYDPAEDKVTLENSSAGDTSLINFGASTDTSNFLAALHLTGAYQA
                     TGDNGATVVTSSRNLGAVDMSAALSDGAFRNGAVTAGSFRINGIQINVDPLTDTLGSI
                     ITAINASDARVTASYDEVSDTLRIVSDTPGSRTISFESGTSNFLDVMNLTTAVQTAGS
                     DAQFKIDGGAIQTRNTNAVTDAIPGVTLNLRGVGTSNVTVETDTDAIVERVRGLLTAL
                     NDALDGIRRQTGRGATLQGDSSISMISSHLQYLMFSKVEGAGDAFSTLLEIGISTGSA
                     FDSEAGGRFELDEEAFLARLRENPESLERLFSNNEQTGIANVLSSYLEGATSTTGFLN
                     QRIRGGGSVDSQIQAYNDRIARLEYIIARHEERYRAQFARLEQMASQYRSQGFSLLGL
                     GSGFSV"
     gene            complement(13746..14954)
                     /locus_tag="PKW60_01330"
     CDS             complement(13746..14954)
                     /locus_tag="PKW60_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPJ98102.1"
                     /translation="MPTFTYVARGRDGKRETGKMTAENRQAVVRTLQGKGMVPERVEV
                     LGGEKKARRDPKVKTSELLVFTRQLSTIVNAGLPLLQGLDILAEQTEDPRYAAVIQGI
                     ADDVEAGETFSDALRKHPRIYSDLFVSMIRAGEASGNLDGVLLQLAEYLESMEELKRR
                     IRSAMTYPVVALSMILLIAAGLIIFVVPQFAEIFSGFGRQLPAPTRVLIAASSFLKQW
                     YGMPLIIAVIVAAVFIVRMYGSTSTGRFHLDSLKLQMPIFGKLLRKVAISRFARTLST
                     LTRSGVNILGAMEIVERTAGNEVFARAISTAADCVRNGETLADPLARSGQFPAMVTRM
                     IGVGEKTGALEQMLQKVSDFYDSEVRAAVDALTSMIEPILILLMGLVVGGIVVALFMP
                     ILQLSGLVQQ"
     gene            complement(15489..>15748)
                     /locus_tag="PKW60_01335"
     CDS             complement(15489..>15748)
                     /locus_tag="PKW60_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPJ98103.1"
                     /translation="LKPGGLFIFFEHVAAPRATALRAVQRLLRPGWQKVGEGCRPDRE
                     LWRALEIAGFTQLTYEHFRVPWALIVSPHLAGWAFKPWNSA"
BASE COUNT         3078 a         4629 c         4710 g         3331 t
ORIGIN      
        1 ggattctgtc ccggcctgcg acagctggga ttccaaatca gtgatcaggc gcgcaccgcg
       61 caagaggcgc agcgtttccg ccaggtcgct gggggccgcg gcgatggggt attccaaggt
      121 gccggagccg tcagcgccct cgaatttgag ctgcagcacg cattcggcgt ccgcagggca
      181 gcttccataa acaacaagcg gtgttccggc gaagaccgtg tctgcggggg ccgggctgag
      241 cctggcatcc gtcgaaccgg caacaataca gcccactccg gttgccacgg ggcggcctac
      301 ggaggcaaac agctcgagtg cggccaggtc tacacgttcc cggggcgtca tgaaacggct
      361 gacgccgccg gtttcgcgcg cgagcaaggc gaggaagcgg tcctggctgg cgctgccgat
      421 acccaggcag tggatccgga acccccgatc gtgcatgccg gccatgatct cgctgccggc
      481 aaagacctcc ccgtcggtca ggaggaaaat atcccctccc ggctcaccaa agagtctcgt
      541 agcggcagac aacccctcca gcagttctgt tccgccccgt gcgtccacgc gtgagaggaa
      601 ttccgccgct ttttcacggt tctccctggt tcccggcacg agatcggcgg caaacacact
      661 ggtccggtcg tcaaaggcta cgatgccgaa ctgatcctcg ggcctcaggg cgcccaggca
      721 cgcgcggatg gccttcttgg cctgctccat gggcgctcca cccatggaac ccgaacggtc
      781 cacgacaaaa gccacccgca tgggcgtatc ggggcgtttc ccgaacgcag tcgaggggac
      841 cacaccgatg aactgggtct ggctgtcctt gtcaatcccc gcgaagacct tcggctcgga
      901 ggcctccatt ttcacgtcaa gcaccaggtc ccggttgggt agatcatcca tgttggcaag
      961 ctgaacatac gcggaatcct catcacccat gcgggccgca agcaagtgag acggggacgc
     1021 cacctgggcg ccacgacccg ggaattcgac caggaggtca aaccctaccg tgtgcaggtt
     1081 ctgggcgtcg gacgaccacg gaggaaggag caggtcgtcg tagcgatctt ccggaagctc
     1141 catctcgccg tagccttccg gcgtgacggc cacgcgggcc ttgcggtggt aactcggcgc
     1201 cagggtgaag gggaaccgaa accggaaacc gtcgtcgtgg tgttcaaccc ccgccaggat
     1261 ctcgagataa acggccaccg tctcaccggg acgcacgttg ccaacgctca ggctcgcaag
     1321 gccgtcgttg tagactcgat tcagaacggc caggtgccct tgcgcgatgc cttcttcgta
     1381 ttcttcccgg gctttctcag tgagtttcag ctcagactcg accgtgaatc cttcacccac
     1441 cacacggaaa ctgcgcagcg tggcatcccg aggcagaccg taggtgtaga ccagctcgag
     1501 gggcttgatc tcggccgatt taaagacatg ccgcaggacg agccgcgccc cgacaggaag
     1561 cacctttccc gtcagccaca attcctccat ggccagcttc acgtcttcac cagtcttact
     1621 gtcgcgaact ttgaactgaa tgcccgagtc ttcttgtggt gctgccattt cctgacctcc
     1681 tcttaggcat catcccgcac attgagcgcc cgttgcagtt ctacgagcgc tttctggata
     1741 acccgaagcc gccacggcgg ggtatccccc cgaatcgtca cggacacatc gggcgcgacc
     1801 tggtaaaccc aggtctgaac cggcgccgct aaaggctgtt ccacctcctc agcaaccgcc
     1861 atcttccggc ggatttgact cagcgtaagc ccttgccgtt gaaactcttt gattgctgcc
     1921 agcgcgtcga gatgctctgc tccgtacgct gcattacggc ccgccctcaa cgggcctggc
     1981 agcagcccct gggtgatgta cagccggatg gtcctcgccg gcacacccgc ccgatccgcc
     2041 agctcattca gcttcatttc ctgaggatga tctctgttct ccgctcccac gccttcctcc
     2101 ttcaacctcg cttcatctga ggggattata tcagacaaaa tatgacagtg tcaagttctt
     2161 tttgattagc caaatctgac actattcgat ggcgaggggc cgggtcccag atatgtcggg
     2221 cagggctcga ttacagcctc aggaaagaga agcggaccgg ggcggcgcct ggtttcatta
     2281 aagggcacct ccggccgagg cttgcttccg ggcgagggga ttctcaaggc atttggcttg
     2341 gggtgcctgg ggcggtcagg ttgggaacag gccagtcacc atgtaggcct gaaggacgcc
     2401 ggattcctca gagttgaaga agcccgtggt cccgtcccgg gagaggaacg ggtggatatg
     2461 ggcagtcttt ttgatggcat ccgtctggga atcaagcacg aattgccagt tcgtgagggg
     2521 gtcgtccggc gccgggccga actccgcggt aaagagcagc cacgtgttgc cgatacgggc
     2581 atcggtgatc agccgtttgc ccgccaggtc ggtggcgaag tggtaaaacg cgggtttggg
     2641 aaagacgcgg ctgaggttat ttcgttggcc gccgggcgtg ctgatgccta tgtggccggc
     2701 aaaggacgca ggaggactca ggatgagttc gcagtggcgt gaatcgcgga cgctcgtgct
     2761 ggtgatggcg agagtggagt ctccgagcca gcactggtgc ccctggcaaa actcattccc
     2821 atcgcggccc caggggaggc tgcggaagtc cgatccatcg tcgcgaatga ggtgaatgtc
     2881 cgcaccatca ccgccgacca ggcgggtgca gttgccgtct ttcgtatatt cagagccgtg
     2941 gttctcctga atgaggatat cgtgggcggc ctcgggcgcg gtggagcggc agtactgggg
     3001 atgcatattg agccaggtag ctccggccaa aacaacgcgg accgctcctt tttcgaggtc
     3061 aaagaccatc agcccgaatg gggcgtcttc ggtgtcgcca tctccgaaga aggccgacag
     3121 tgccagccgt tttccatcgg aggaaatggt ggaaagcgga tagatgtggc tgggaatgca
     3181 cgccgcgccc ggagggggcg catcgaggac catcagggtt tcgcggtcgg acccgtccag
     3241 acggacgcgc ttgagcgtca actggccggc attcaggctg gtttcgttta tgaaataata
     3301 catataagtg ccgtccggcg agacggaagg tgcggtcacg ccgagctcat cggtaagagg
     3361 cgacagttct cccccattct ccaggtcgca gagaagatag cggtgttcgg gatccgactt
     3421 attgctgccg tggggatggg cgcttcggtg taagacaaaa cgcttcgaat ccggcgtgaa
     3481 gatttgcgcc tccatgtaga catggcacga gggtatggcc tcgtcagtga gctggaacac
     3541 ctcagcattc gtcggcgcgt ctttggtcag cagatcgggg cgaggcttca tcgagagtcc
     3601 tccgattgtg tcgtgttttg tctactatag cgggtttttg agtaaaactc gcccattcga
     3661 atactaagtg tacggttttt gcttttattc tttcctgttg gtgagtctta aggcaggagt
     3721 cgtcaaggaa gaaaacctcg agaccccttc ggtattccta gaggtgacga tcaaccaaga
     3781 gcgaatggga tcccgatggc ctacaccatc ttaccttaat ggagcgcacg aagatcgaat
     3841 tttttgtcgc acaggacttg gctacgctgc ggttgccctc gaattgggcc gacttaacga
     3901 gtcacatcgt tttgacgctc attttggtct gaatcaatcg cctggaggtc tcggtttctt
     3961 ttttcggtaa gttgaataac gcggagcctg ccgatttttt atgcgtattt tgcagtcttt
     4021 agacatgctg ttgttcgccg cttagctctc tcctcttgtt catccgtatc tctaaggcac
     4081 tgaatctcca atgcagctat gcaagagatt gctcgattct gtgaatcact tcgaaaggcg
     4141 tctcaaacac ctctcgcgca ggaatgcgca ggacctcata tccctggcct tggagatatg
     4201 ctgtgcgggc cctgtcctgc tcgatcatct ggaggtgtga tcgcccatct acctcgatga
     4261 cgaggcggag tccgctggcg gacttaagaa tgaagtcaac ctcagtatcg ccaacattcg
     4321 gatgaatatt tggctcccac cccttgacga ccatccagct gaacagctcg agctcggcgg
     4381 gactcgtgtc tcgaagtaat tggatgtctc gacaatagag ggccagcttc cgcaggaagc
     4441 catcctgctg gaggcagtaa tcaatgtcac ccacaacgaa gacagcctcg cgggctcggg
     4501 tgatagcgac gttgattagg tttggtggag attccaccca acggcttgcc gagaccgtaa
     4561 tacccttggc cacgactggg ctaaagatta tcacatcgcg ctcatcgccc tggaatccgt
     4621 aagctgtatc gacgaggacc tcggaggaga gctgcagctt gtccagcctt tcccggagcg
     4681 tctctttctg ggcggcaaac ggtgtcacga cgccaaggct gagcgaacgt gaatcgccct
     4741 gcttaagctg ttgaacgcgc tttaggacct cttcagcttc cggcacattg acccagctgc
     4801 gatcattcgg tccgcgcttg gcaagacccg taactggaaa aacatggacg ccgctgccga
     4861 tcggcaatct tgcgccccaa ctcgggtctt tcttaagttc gaggcgctgc aagtagatgt
     4921 atcggtttga gaagccgatg atctgcgggt tgctgcggaa gtgttcattg agcatgagaa
     4981 catccgaacg cctgcgcgga agcgacgcgg tcgctgtctt gtagacatca ttcgtagcgt
     5041 gcccgaccag cgagaggtgt tcctcgatgc cgtatttccg ggcgagcgcc agctcctccg
     5101 attcctggag ggtcggtatt gccggtagct ggtgttcatc tccaatgacc gtgagcgtcc
     5161 gggcacggta catgagaggc agcaagttgg tgagcgtaca ctgcgacgct tcatcaataa
     5221 tgacaatgtc gaagagttca gaagcgagag ggatagcctg tgatgcttga gcagttgtga
     5281 tccagatcgg cacggcacgg agcgcggcac ggaaggtctg gtattcgctc tcgacaaaga
     5341 ctcccctatg acgccggagc gctctctcca gtcgatcgac cgcacgaata gcatcatcgt
     5401 cgcctcggat tcgctcgagc cacttcgcct tagcatcctc gaatgaaccc ttctcaagct
     5461 ctacctcaat gcgtccaatc ttcgccctga tccggtcagg gctgaattcg gcgaacgcgt
     5521 cattgaggtc gtagactggc caatcactct caggctcttt cttgattaga tcgaagacct
     5581 ttgtgacctg gatccgctct gagccctcgg ggagagcttc aaccgcctgg ccaagcttgg
     5641 cgactgccca ctccaactcc tgttgggcct tcgcctccat gcttggtcgt gtggtcttgt
     5701 cgaatgcgcg ccagcggtca cataaggccg acacgacctc gatctgctcc cgggcttctt
     5761 caatatcggg ccagttcaac gattggccgg caaggacgaa tgcgctgttc ggacgctcgc
     5821 gccaccaagc cttgacatgc tccaccggct cgggcacggc tttaaagtca gcccgcagct
     5881 ggctcgcggc ctgctcatgg tcactcgcag cctgccaaca ttcaacgaga tgcgttaggc
     5941 ttccggtgta aaccgcagcc cgcgcggcat tgacggtctc ttgcccaggt gcatcggcca
     6001 ggtcgcggag ggctccgtcg aagtttacgc cttgcccacg acgccaggct tctcggatgg
     6061 gtacgccgct cgcaagcctt gaccactctt tcgcaatcgt ccgaagttgc tcttgtgtct
     6121 cctctttttc agtacgtcgc ttccatgctc gtgctgcggc gtcggcaagg gatgcggtcg
     6181 tttcgcattg ggtaacgata tgcgcccacg cctcgatgct cggcgagctt ggtacgtccc
     6241 ggagccgcag tgcagcggcc tgcgatcgaa gctcctgaaa gcgatcttcg atttccccga
     6301 tcagcgtctg agcggattgc cacgcgtctc gaagttcaat ccaccggcct attacctcaa
     6361 tgatgggtgc gatctgagcc cggccagcgt cattcagcgt gccaatccgc gtccagaatt
     6421 ccaggggcaa gcccgacctt atctgttttt cgtggcggcg aagactgcga cgcaggcgcg
     6481 aaagctcaga ccacgggagg aagtcgaacc atcgaccctc atgcgtcacg tactcggcaa
     6541 agtcggcctt ccagccccga agatgctcca aaggaatttc cacatcggtc ggcatgccga
     6601 tcgcactgac cttggagcga ccttcaccga cagcagcgga aagctgccgg atcttcccta
     6661 gcttcggggc gagtttggcg cagctgcgga gcacgacctc agcaaactcg cgcgcctcag
     6721 tggcccagcc attagcctgc tcaccagacc tccagcggtc gaactcttct cgccattcta
     6781 tgggttccag tgcctgctcg acgggtttgt caagtatcac gcgaagccgc tgatcgaaat
     6841 ccgccaggat ctctgggctt ggcccggtga gcaaccacct ccaatcccca gcttccgcca
     6901 cggacaggcc gacttcctcg atagcccggt cccggtgccg ctctcgatcg gcaatttctg
     6961 ccaggagttt ctgtcgccgc tggttgtccc gtgcaataag atccttaaaa agctgcatgc
     7021 gctggagcca gcctgccgta tcttcgaccg acgactcgat cttcgtgaaa cacttgtcct
     7081 cgaatcccag cttttccttt tccacctcga gcgcgtggtt ctgatccgca atcgaggcaa
     7141 gcgttttgcg gtactcaccg tatgcccgga gcgcggtttt cctagattcg tcgatgcgtt
     7201 gcggaagctt cgtctctagt tcactttgta gctcgcgctt cctgtcaaga aggtggatcc
     7261 gttgccgacg gagtctctcg acgtcgacac ccagagcatc ttctccgttg gtcacggcgg
     7321 ccatgttgag tgtgcgtcga agaacttctt gaatgttctg cttgtgtctt gctcctgctc
     7381 gcaccgcaat cgggaactcc gactcgaacc tctcgacgcg ttcgcgaaca acgtccactg
     7441 ctttgttgtt attgctcgcg aaaagcaccg tttgcccgcg ggcccaagca ttgagcagaa
     7501 gcgaaacaac gacttgactc ttgccagtcc ccggcgggcc gctgatcaca gtgagcggat
     7561 gctcctcgag aatgcgctgc accgccatcc gttgtgaatc attcaggggc acaagcggga
     7621 gaacctccgt ctgcgtcacc ggcaatgtcg tgggctggtc ttcgagcagt cggaggccgc
     7681 cgatctggtc cggatttcgg gtcaggagtg cttcgagacg ctcatagtcc tgcatcaggt
     7741 ggcgagtgaa tgctgagaag tcacgggtcg gggcgaagag cacccaagga gtgggctgaa
     7801 catcaaaggt atcgcgacgg atcttctttg tgaactcggc ctcagtctcg ggaacctcgg
     7861 agaagactgc ccggaagatg cagcggtcga gtggtgcctg ttctagttgc cgataaccgg
     7921 cggccttttc gatgagactg gccgcgaact cgtcgggtga atcgccgaca ctcgtctcga
     7981 gttgatcgag caggctgtag ataagcggcg taacactcca agagccttct cttggcacaa
     8041 tctcgaagcg cgagccattg ggccgaagtt cgacctcccg atagaggaga gggcgccatt
     8101 gaggcatcca ttcttcgcgc atagacttcg accagccgag gaagcagggg ccaccaagaa
     8161 agaggctctc gtgctggcct tgttcgaagg cgagccggaa tggcaggctg cggataccgt
     8221 cgcagtcgat ctgccatgga cctgcgagat cggatggttc gaagttcggg aggctcgtcc
     8281 atcgttcggc ttggtcatcg cgaccggaaa gggagaagaa cccctgctcc gcgcgtgtga
     8341 cgacctcctt atggaagcgc accactgaca accacgggat atctctgcct ctatatgcgc
     8401 tcatcgtgat tctcggatta gtcttgcggg ctggacattt ttttcacact cccaatggtg
     8461 tgtcagatac tgggttttgg aataaagaat tgatttcttg aaaaaatcaa tactcatgct
     8521 caaatctaac cccagtgcct gcaccaaatt cgcatgacgg agtcacggta gaaccgaata
     8581 ttgtggagtt ttcttccatg ataccaccgg cccttgagac cctggcaaat cggcagcaac
     8641 cacgccgagg ccaaagcgtg ctcaagccaa tctgaattca aagcgcatag ggagaacatc
     8701 cccccctttt gagaattgcc taaaataggc gaaacccgcc aaaagttcaa ctgcttctcg
     8761 caatcgccgc aacctgacgg cgttgtcttc caggcggccg tatggcattc ccgctcagct
     8821 acacggagca attatgtcgg gagcgctggg atccgcgtgc ttgcatttcg ggtgcttcac
     8881 gcaactgcgg aggaactcgc cccgccttca tgggtcacat gagccgcagg gccgggcagg
     8941 attttccggg gcatggggag catcgctcac gaggtgatgg cggcggtgcg gcctgacatg
     9001 aaaaagcaga gggtttccgt cgtctgccgg gctcaaagcc ggggcggtgg agggtcattg
     9061 ccgagatttt gaaaggagta ccgagcatcc aggcatggct tttttcaggg cagccgcacc
     9121 ctcagcgctg acgccggtgt tggccaggtt gacctggcgc aacgttggga tttgtgccaa
     9181 aaccaagatg ctggcatccg tgatgtcggg cccatccatc tgaataaact gcaggtgccg
     9241 gcattgggcc agatgttgca acccttcttc gttgattgag ttgccggcaa ggtctaaccg
     9301 ttgaagactt cgaagcgctt gcaaatggac caggcccgga ccggagactt gcgtgtggga
     9361 cagattcagt tccgtcaact gagagagctt ggcgagcgac gccagtccgg catccgaaac
     9421 cgccgttcgt gagaggtcga ggcggcggag cgccgtgaga ccggcgaggt gttgcaggca
     9481 ttcatcgttg acattgcctc cccgcaaggt gagccgttcc agcgaggtga gcgctgaaaa
     9541 ctttgccatt ccagcggcgt catatttgtc ggcgccagcc agggaaagct cccgcagtcc
     9601 agtcagtgcg cacagctgag tcaggccctt attcgtaatt tgcgcatcat cggctagaag
     9661 agagcgcaaa ccggtaaaac gggccacgat gggcgcggca tcgtcttcca ggcgggctgc
     9721 gctcacgtcc agcgaatgca ggtcatccgg gctcagcagc tgaatggcgc ggagctgcgc
     9781 ggagccggct ttcacgacca gctttacgat ccgctcggcg gggacaacca cacgcccctt
     9841 ggccggttga aacgacttcc agtcggtgct gtcgagggca tcccacgcct tgacctgcac
     9901 atcacccacc atatccgcga agatcagctc acggggcccg ggcggcgtcg ctggagtgac
     9961 aggttcgggc ggtttggggg tgggcgcctt cggcgcaggg gctggcgcgg gaggctcgga
    10021 tggcttgggg gcagcggtgg ccacaggaac gggcgccggc gcagcagcag caggtttcgg
    10081 tgcagacgcg acgtcaaacc gcaacgtgca gtctggcagc cactttcgca ggcgggcaat
    10141 cccttcttcc gacagctggg tgtcccggac atccaggacg cgaagctgcc gcaattcggc
    10201 caacgcgtcg atcatctcgt ctgtgacgtt cgatttgctg aggtcgagct gtttgagcca
    10261 ggaggctttc tgcagcagaa ccaggtcggc ttccgtgagt tcaggggatg cgttcaggcc
    10321 gatgtattcc catttgggga acgacgtttg tccctgggcg gccccatagt cgcgccaacg
    10381 cacatgtgag ccgtcaattt cgcgaatcac aagggtgccc atcgtatgat catcaggaaa
    10441 tacgaactgg cgcggcccaa acgccaccca caggatcagc acggccccgg cgagtgccag
    10501 cgcaatgact gcgatcatcc cgttggagat aaaaggcttt ttcgcgggct cttcaaccgg
    10561 ggtggtctcg agcgcaggcg gcgtggacgg acccggcacc ggcttctctt cgaattcgct
    10621 caggggtgga ggcgtaatga acggagtgac ggcatcagaa agaggcgtcg aataggagcc
    10681 ggccgagctg tatgagctct ctttctcggg gctggagtca tacataaggc ccgccggcgt
    10741 agacgacatc cacgacgagg tggtgtcgct cgcaggcgtt gctgcctggg ttgaagaatc
    10801 ctcggccgca ccttcttcgc gaacgccaat atccggcggc agctctggcg gggcctcttg
    10861 aaacgtgttc tgagggatgt aggagtccgg gcggtcgccg atacgttcga gcagcgtcgc
    10921 tgcccgttca tcctggtgcc tttcggccaa ttcctggcat agcgcccggg cttcctggta
    10981 gcggccgact ttggcaaaac acttggccag agcatataag acgtgtttac tgtcagggaa
    11041 ttcgagggcc aggtcatcca ggatggccat agcgtcctca tagcgctcgg tgcgatagaa
    11101 catgagcgcc atgcggtagt tatgttccgc tgccttatcc gacacgaggg ccccttcttc
    11161 gcatcacagc ttagccaaaa ccgtcaggtt tctgtactaa gtatactatt ctcatagctt
    11221 gtatacaaaa cagccaaccg ggcctgccac cgccaccggt tgaaagccac ggctcaactt
    11281 tccacgggcc caatcgcccc tggcgccgga tagaatacca gtctcgggtg gagaaacgct
    11341 atcccctgag attcatcagg gaggccccgt gctggtgcat ggccgggggg tttacaggct
    11401 ggccctccgc cgcgagcttt tcaaaggcct ctttccaggt atcgcgcagc gttgtcaggt
    11461 gcgagagaca ttcgtcgatg ggctcgatct ctttgcggag gtttgccgta atgaggaggt
    11521 gctgaaggta ttcgtagatg cggtcgaggt tctgagcgat tgcgccggtt ttcatgtcca
    11581 gggcactgcg gagttcgcca aggatttcct gggcccggat gaggttgttg tggacgtttc
    11641 cgatgcggcc cgccgcaata tggcgcttcg cctcttcggc tcgctgaatg gcgccattga
    11701 acaacagcac aatgagcttg ccctgggacg tggtctcgac gtccaccctc ttgtaggcgc
    11761 ccagctgtgc ggtggtttga gcatctccca ttgcgcctct cctgccaagt tataccggaa
    11821 acgccggact acaccgaaaa gccgcttccc agtcccaaca acgagaaccc ctgggatcgg
    11881 tattgcgaag ccatttgttc gagccgggcg aactgggcgc gatagcgctc ttcatgccgg
    11941 gcaatgatgt attcgagccg ggcgatacgg tcgttatacg cctgaatctg agaatcgacg
    12001 ctcccgccgc ctcgaattcg ctgattgagg aatcccgtcg tggatgttgc gccttcgagg
    12061 tagctggaaa ggacgttggc gataccggtt tgttcgttgt tggaaaaaag ccgctcgaga
    12121 ctttcagggt tttcccgcaa cctggcgaga aacgcctctt catccaattc aaatcgcccc
    12181 ccggcttcgg aatcgaacgc gctgccggtg gagatgccga tttcaagaag ggtactgaat
    12241 gcgtcccccg ctccttcaac cttgctgaac atgaggtact gaagatggct ggagatcatg
    12301 gaaatgctgg aatctccttg aagcgtcgcg ccgcgcccgg tctgcctgcg gattccgtcc
    12361 agcgcgtcgt tcagcgcagt caggagtccc cgcactctct cgacaatggc atccgtatcg
    12421 gtttcgacgg tgacgttgct cgtgcccacg ccacgcaggt tgagagtgac gcccggaatg
    12481 gcgtcggtga cggcattggt gttgcgagtt tggatcgcgc cgccgtcgat tttgaattgc
    12541 gcgtcgctgc cggcggtctg gacggctgtg gtcaggttca tgacgtcgag gaagttgctg
    12601 gtaccggatt cgaaggaaat cgtgcggctg ccgggcgtgt ccgagacgat tcgaagtgtg
    12661 tcgcttactt cgtcatacga ggccgtaacc cgcgcgtcgg aggcgttgat tgccgtgatg
    12721 atgctgccca gcgtatcggt caggggatcg acattgatct ggatgccgtt gatccggaag
    12781 ctgcccgcgg tcaccgcgcc gtttcgaaac gcgccgtcgc tgagggccgc cgacatatcc
    12841 accgcgccga ggtttcgcga actcgtgacg acggtggcgc cgttatcgcc ggtagcctgg
    12901 taggcgccgg tcagatggag cgcggcaaga aaattgctgg tgtcagtaga tgcgccgaag
    12961 ttgatgaggc tcgtgtctcc ggcgctgctg ttttccagag tgactttgtc ttcggccgga
    13021 tcgtagtgag cctgcacccc gatgccgctg ctgttgatct cggccaggat atcgttgagg
    13081 ctctgggtgt cggggtcaat ggtgaacgtt gtgccgttga tggtgaagct gcccgcggtg
    13141 acctcggtat ggatgccgct gccgctgagc gcggcatccg gggagatggc ggcaccgagc
    13201 gcgctgctgc tgcgggcaat ggtggcgctg gcgagttgca acacctcgac cgtgtacacg
    13261 cccggtgcag catttgcgcc tgtaagtgac gtcgccagaa cggtattttc gctggaagcc
    13321 gtgcggaact ggccgaacac cgtgttcagc cggaaatctt ccgcgcggtt gcgcagggtc
    13381 tgcagttggg tgcgcacacc gccgatggcg ccgcgctgtg tctccaaagc agcaatgcgc
    13441 tgctgaagcc gcgcaattgg ccggcgctcc agcgcgatga gctgtgcgat caatgcgtta
    13501 gtatccagcc cggtaatgag tccgcccgcg gaaaacgctc ccgacatgga ccatcctcct
    13561 gcaccatgcg cgactttcac ggtttagata gcgtacagac gctacctcac ccgatattat
    13621 cgaccacgca acccttagac tttagcctat ttggccggtg aaccccgcaa ttcccgcacg
    13681 ggcgcgacca ggctgccttc attccgaaca tcatcccgtc catcaggcga gatcccggcc
    13741 ggttcctatt gctgcacaag ccccgagagc tgcaggatgg gcatgaacag ggcgaccaca
    13801 atgccgccca ccaccaggcc catcagcaga atcaagatag gctcgatcat gctggtcaag
    13861 gcatccaccg cggcacggac ctccgaatcg tagaaatccg acaccttctg cagcatctgt
    13921 tccagggcgc cggtcttttc tccgacgcca atcatgcggg tcaccatcgc ggggaactgg
    13981 ccgcttcgcg cgagcggatc ggccagtgtt tcgccgttgc gcacacaatc cgccgctgtg
    14041 ctgatggcac gcgcgaagac ttcgttgccg gcggttcgct cgacaatttc catagcgccg
    14101 aggatgttca caccgctgcg ggtcagggtg ctgagcgtgc gggcgaagcg gctgatggcc
    14161 actttgcgga ggagtttccc gaaaatcggc atctgcagtt taagtgagtc aaggtggaat
    14221 ctgcccgtcg atgtcgagcc gtacatgcgc acgatgaaga ctgccgcgac aatcacggca
    14281 atgatcagcg gcatgccgta ccattgtttg agaaacgaac tggccgcaat cagcacgcgg
    14341 gtcggcgcgg ggagctgccg gccaaacccc gaaaagatct ccgcaaactg cggcacaaca
    14401 aaaatgatca gccccgcggc gatcagcaga atcatgctca acgctacaac gggataggtc
    14461 atggcggagc gaatgcgccg tttcagctct tccatggatt ccaggtattc agcaagttgc
    14521 agcaacaccc cgtccaggtt gccgctggct tcacccgcgc gaatcatgct gacaaagagg
    14581 tctgaataga ttcggggatg tttccgcaga gcgtcggaaa aggtctcgcc ggcctcgaca
    14641 tcgtctgcaa tgccctgaat aaccgcggca tagcgcggat cctctgtctg ttcggcaaga
    14701 atgtccaagc cctggagcag aggcaggccg gcgtttacga tggtggacag ctggcgcgtg
    14761 aacacaagca gctccgaggt cttgaccttg gggtcgcgcc gggccttttt ctctcccccc
    14821 agcacttcga cacgttcggg aaccattcct tttccctgca gtgttcgaac aactgcctgg
    14881 cggttttcgg ctgtcatttt gccggtctca cgttttccgt cacgaccgcg cgcaacatac
    14941 gtgaaagttg gcattgcgga attcctcccc gttggaccgg ctcctagtat acccatacca
    15001 gatgctgtgg tcgaggctca tatgaacccc gccgcttggg catgccgtca cagggccgga
    15061 gcagacagca aaacccttcg cggatgacct gtcgacaccg ccacgtctcc cctcgcaagt
    15121 ataccaccgc tttagatgcg tcggagcgct ggcgttgcac cacggatacc ggcggtgccg
    15181 ttcgcgggta acctgcttcc gcaggaagca ggctctaccc tgctggaaat gagcaagttt
    15241 cgggccagcc gcagatttcg gccgattttg tccgcagttt actgtaaatt gtttaattta
    15301 aatatgttaa gtacttcttt cggcaaaaga tgcgatttat ttcccagcat tcgggcggcg
    15361 gtttttcgtc attctgttca gctgccgtcg ttcccccgtt gctctgccgg ggatacgagg
    15421 ggctgagcag gacccgtatt tcgtgatttc catggggcac agcaggccga tcggtcgcgc
    15481 gggggtgctc aggcggaatt ccatggttta aaggcccatc cggcgaggtg tggtgagaca
    15541 ataagggccc agggcactcg aaaatgctcg tacgtcaact gggtaaatcc ggcgatttcg
    15601 agagcgcgcc acagttcgcg gtcaggacgg cagccctccc ccactttctg ccatcccggg
    15661 cgcaggagcc gctgcacggc gcggagcgcg gtggcccgcg gcgcagcaac gtgttcgaag
    15721 aaaatgaaca agccgccggg cttcaaaa
//
LOCUS       DAOPBQ010000018        15554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_3866_length_15554_cov_3.712239, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000018 DAOPBQ010000000
VERSION     DAOPBQ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 15554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15554
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3866_length_15554_cov_3.712239"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="PKW60_01340"
     CDS             <1..1198
                     /locus_tag="PKW60_01340"
                     /inference="COORDINATES: protein motif:HMM:NF019944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HPJ98104.1"
                     /translation="VMASRADQDIKYWRAVQGELQRMLNEFTFVHRSLVYIHREEESL
                     FSDEQRPNKAAVTLDVSRPLSKNEIQAVLGVVSSFGGANLAPENITLATTDGKTLNLP
                     SEDEFTQISNRRLDFLNEYERQRELKAETALAKMGVKGFVRVSLDINSSSVEETATKT
                     EKGTPVSEMLTRTETTSRESLPEGPPGTLANIPEGAPGALETTETQREEIFNYEPSIT
                     KTTTITPPGAVLKARAVAVVSGAYQPVIGADGKPTGDTEYIPRTSEQLEAYRTVVATA
                     VGPELTPEEVSVFDHPFEMEQHAAIEVFKAFEWAQIQENMIKWGSTALKVVLVLVGFL
                     VFRHYLMRVLVFSEGEEEVLPPQHEPTLREMRRREMAEEVERVSTEQPEQVASLLRAW
                     LAEGEE"
     gene            1235..>1833
                     /locus_tag="PKW60_01345"
     CDS             1235..>1833
                     /locus_tag="PKW60_01345"
                     /inference="COORDINATES: protein motif:HMM:NF026192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HPJ98105.1"
                     /translation="MSALDGNTKAAILLACLGPTAASRVLSRMDDSEIERLTLDLSSL
                     GTIAPETREAIVEEFHQMMVANRYVTQGGVEFAKNILESALGPERAVEILARLQSSLQ
                     EVPFEFLKKADPSQVISFIQDEHPQTIALILAHLQPDIAATVLSSLNSDVRTDVVLRI
                     ATMDRTPPEIVREVERVLERKMASVFSQGFTFAGGVKEVA"
     assembly_gap    1834..1933
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1934..2400
                     /locus_tag="PKW60_01350"
     CDS             <1934..2400
                     /locus_tag="PKW60_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HPJ98106.1"
                     /translation="ASVFSQGFTFAGGVKEVAEILNRIERATEKNIMAELEERDPELA
                     DEISRLMFTFDDLIYVDDGGIQKALREIDQKDLALALKHVDDEVKEKILKNMSTRARD
                     MIAEEMEYMGPVRLRDVEEAQQKMVGVVRRLEEAGEIIIEGRGGSAEDDIIV"
     gene            2439..3089
                     /locus_tag="PKW60_01355"
     CDS             2439..3089
                     /locus_tag="PKW60_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HPJ98107.1"
                     /translation="MAKIIKSWDMSAQEYPSYRRTALETLKTELHRSSGTLALDPRQI
                     LEDARHEAELKVREAYAEGLRRGTEAGQAKFAEAVGKAAEALESAALAMREAQAEFLL
                     SLEPQVVALAGAIAERILRREPSIEPERVRLTAHEALAHLEKRERITLRMNPADLEAL
                     KEQNISLLEEFDAIGDLEIIADDSVTPGGCVAQSELLEVDARMESQFQKIVDALLE"
     gene            3152..3857
                     /gene="fliI"
                     /locus_tag="PKW60_01360"
                     /pseudo
     CDS             3152..3857
                     /gene="fliI"
                     /locus_tag="PKW60_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518241.1"
                     /note="involved in type III protein export during
                     flagellum assembly; frameshifted; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
     assembly_gap    3858..3867
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3868..4392
                     /gene="fliI"
                     /locus_tag="PKW60_01365"
     CDS             <3868..4392
                     /gene="fliI"
                     /locus_tag="PKW60_01365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200539.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HPJ98108.1"
                     /translation="AQREVGLAIGEPPTTKGYPPSVYSLLPKLLERAGTSANGSITGI
                     YAVLVEADDLNDPVGDAVRSILDGHIALSRDLATRNHYPAIDVNESVSRCMIDITSEE
                     HRRLAGELRHILATYRDAEDLVNIGAYVAGSNPDIDRALRLMPQVRAFLRQGLYEESP
                     FTEIERLMRNALQG"
     gene            4400..4861
                     /locus_tag="PKW60_01370"
     CDS             4400..4861
                     /locus_tag="PKW60_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HPJ98109.1"
                     /translation="MAPGKPFRYDTLLRVRQRQEELKSMALAEMRRAVDFAEQQRAEI
                     VEQQFAALNAAGELAQRQFNASEVRRYFSYERHLARLAVEKDAEIRHLRTQERGRRKE
                     LEAAMKRRKMLERLKERLEEAYDYVVNREERKRLDETAVNRAALADGTQKP"
     gene            4858..5424
                     /locus_tag="PKW60_01375"
     CDS             4858..5424
                     /locus_tag="PKW60_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98110.1"
                     /translation="MKALVTAVVFVATFVATVLLLLLFTGNLNREGLARLRRGGEGAA
                     PPAPVVRAADESDALLNALRTKEQQLAEREAAIQEEEKRLAQVRRELQTLRDSLETMQ
                     KKVNESMDSADADARTRMEAVARSLASMEAKNAAQTLTSEMFTPDEAATILQCIEEDR
                     KRGEILNEMQPNEAATVLQALKRHSFGS"
     gene            5655..7301
                     /locus_tag="PKW60_01380"
     CDS             5655..7301
                     /locus_tag="PKW60_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HPJ98111.1"
                     /translation="METSPVLLSLIPTGQGIEGGLDAGWQEGSGVSGESASFALVLAQ
                     HAASNIAATLGDPALALPSEDLQLESGQPRAPSGAGLLESTQPRSPSDTARSECDQPG
                     PPTGAVLVECAPPQTLSEVAAVPPLLSQANYVLRDVEATESGETEAAAKEEDHDAGAL
                     LVPPFPEQVPGQDGAVTRLSDGHSVESDPTESPVTLRSTATYGESPSAAWGRAGGQEG
                     QAVDARTLRGEAKSPFVLRLAPPKGFDPPVYDTGGGPPVLSAPATVVFGLQPAREAFV
                     EAAPELPSAGTAAPRTPPVEQEQIARLPEQPSDLEPGFPDPKNAMESRPRVLEESLVQ
                     LSTSETVESDISDAPPVQDAPSSAEPASANHSSAGAGPVAIPAGASQAAGPTPAGVEV
                     VEFHASPEPALRDSLAELAVKSVRYLVSEDKRSLRIRLVPESLGEVRIELVSTRNELH
                     LKLVSGSASVREAMESASDALRHALTKDGVHLVRVSVAGDGAVSSGQGGFPERGAWAN
                     DSPGHRYPGSAARSYGNPEAHRSGPAVRAAYHDGRLSVYA"
     gene            7369..7905
                     /locus_tag="PKW60_01385"
     CDS             7369..7905
                     /locus_tag="PKW60_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HPJ98112.1"
                     /translation="MNALVAVRGNFSGDLSPNPVDRAKAATELQSMEKARLTHVLKQA
                     GLVLKSKGAEEAHAMKGNLDRDAFLRLLVLEMQNQDPLNPVDNTEMIAQLAQFSSLEQ
                     MNNLNESFEALSGNIDQLNFISASGLVGHEVTGIDQDGNVVQGTVQTVHLDQSLVYLN
                     VDGKLISMAGVLSVGHAP"
     gene            7902..8303
                     /locus_tag="PKW60_01390"
     CDS             7902..8303
                     /locus_tag="PKW60_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HPJ98113.1"
                     /translation="MINPVQAANMPERLAAGRLSQASATLPQTPFSDVLAQAQRTQSA
                     LRFSAHAARRLRDRNIALSEADETALRESVDRAAAKGSRETLLLTENAAFVVSVANRT
                     VITAVSRSEMADAVFTNIDSAVVVAPGPARS"
     gene            8394..9713
                     /locus_tag="PKW60_01395"
     CDS             8394..9713
                     /locus_tag="PKW60_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HPJ98114.1"
                     /translation="MGTAIYTGVTGLQTHQRRMDVIAANIANVNTPGYRAARALFRDL
                     FSQTLEGGRGPVDGFGGSNPVQIGLGVQLGTVDTIHTQGSLQTTGNNSDLAIQGTGFF
                     VLSNGISQYFTRDGSFTLNALGQLIDPATGMRVQGYLADETGEVNVSAIPTDLEVPVG
                     GTSIVRATEQVMVRGNLDSRAVAGTAVRRRVSVFDSLGTPRELMTTFRKIPQVGVGGT
                     NYNAWSYIVEYTNTDSPPVTTQVGEGVVLFDVEGNYFDEGTLVGGVFTTRPAGTPEVS
                     VPLAAFGSLDSYPDTPFEFEINFSSVTELASDSDATVTNQDGFARGTLESFNIARDGT
                     INGVFSNGLTRVIGQIALASFANVGGLERDGSNLFRDTPASGTPQIGLPNTGGRGQVS
                     GGVLEGSNVDLGTEFSNMIVTQRGFQANARTITTADTLLQETVNLVR"
     gene            9928..10695
                     /locus_tag="PKW60_01400"
     CDS             9928..10695
                     /locus_tag="PKW60_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HPJ98115.1"
                     /translation="MDIATLVGLISGVTLVVVSILMGGNPGVFWSSSSVVIVMGGTLA
                     ATLINFPLGDVLSVMNTVKNAFFHKDVPPESLIPRLVSFATIARREGILALESHASEA
                     EDEFLEKSVQLAIDGTAPELIKDILTTELAFMEDRHAMGQSILVAMGTYAPAFGMIGT
                     LIGLVQMLATLDDPSKIGEGMAVALLTTLYGALLANMVFLPGAGKLKVRTSNELLAKE
                     LVIEGILSIQSGDNPRVVEQKLKAFVSPAVRRQVSAV"
     gene            10697..11479
                     /locus_tag="PKW60_01405"
     CDS             10697..11479
                     /locus_tag="PKW60_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HPJ98116.1"
                     /translation="MPKGKRNKGGEEQPGAPGWMVTYGDMMSLLLCFFVLLLSFSSVS
                     EKKYSQAIHSLKGALGVLPRNLSVIQPLPVEQMAPKRQRTIERIGRMIQERLLINNKA
                     EDIKIEFDEEGGLKINLPSRILFDTARAELKPDAFPVLEDIGMVLKDLPGASIEIRGH
                     TDIRPLTPGGKFEDNYHLSYGRAKSVMDFLHERAQIPLGVFEVIACGPSQPIATNETE
                     EGMQANRRVEINVRGDFSEETTSELRKSIDQMQSGIGLKPEQ"
     gene            11495..12031
                     /locus_tag="PKW60_01410"
     CDS             11495..12031
                     /locus_tag="PKW60_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98117.1"
                     /translation="MADESQPAVPAPKKSSFIGLLIWLSLAFMVAVLGALALYFVIVR
                     PRLTEEALPADAASADKLNPDSVTVDFEESVASVKMPEDSQLPASLLSFQVSLECNDP
                     ATAALIGKHMARFTDMINKQHEFRTRAELDDPRVKTEIQKTILLEANTILKQLQPGPP
                     NPNMRVTAVFHKRFLVSD"
     gene            12037..12396
                     /locus_tag="PKW60_01415"
     CDS             12037..12396
                     /locus_tag="PKW60_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HPJ98118.1"
                     /translation="MEAEAKQPHEHEFREVNPGGVYGTERVSLDDLREVKMTLTAHLG
                     YCSMTVREVLGLRRGSVVSLDRQAGEMTDIYVNGLPLARGEVVVIADVLHVRIGEIIG
                     QEDRSGLEEAHEDAASK"
     gene            12374..13252
                     /locus_tag="PKW60_01420"
     CDS             12374..13252
                     /locus_tag="PKW60_01420"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HPJ98119.1"
                     /translation="MKTQQASSGARLILAAALVLGCVWGVCAQDPASEPVETLVQPED
                     NNEYEATLPPQLDLRSDLTPTVPPEPVPEIPAVVEPAEPPVEPSAESPDPFMEQVNRE
                     LAARRGTAPDSAGANEPAAFPVTERRPRSLLRAFAWLLVVIALILLVYYVLQRRGRGG
                     PLRLGSQLGTVLGRIYLSPRMCLHFVRTGGKVLVVGQTQNALTLIADFNEAEFAVPVE
                     SAAQGAAGEAPAEPKADFLSLLRDGISRIYRTGVEHDQASEPVEPVGEDDIAALRGDI
                     HRLQEYLRDSMREPEA"
     gene            13236..14018
                     /gene="fliP"
                     /locus_tag="PKW60_01425"
     CDS             13236..14018
                     /gene="fliP"
                     /locus_tag="PKW60_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009438664.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HPJ98120.1"
                     /translation="MNPKPKLLLMALAGGAALLVSAGALAQEAPGGTGPLGIENVFRG
                     LEGAAAPDRISTTLALFILLTVLSLAPAIAVMTTSFLRIVVVFGFLRQAMATQQTPPN
                     QVLIGLALILTLFVMSPVYRQVYDRAYRPYVEEDIGWQEAVDNTIGPMRDFMFRQVRA
                     KDLALFIDISKMERPRTRDDVPTLTLVAGFVLSEIRIAFQIGFIIYIPFLIIDMVVAS
                     TLMSMGMMMLPPVFVSLPFKIIMFVLADGWYLLIQELVAGFK"
     gene            14033..14509
                     /locus_tag="PKW60_01430"
     CDS             14033..14509
                     /locus_tag="PKW60_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98121.1"
                     /translation="MDRKLAAIIAGIVFLAILPLVCQVTKDPVPITVEDIKAAFRERN
                     FSIANEHTVNRPRYQADAEIFMTINGAPVYLFTYSNVTVIEREMKNMEADAKRLPAAL
                     GSGNAVKAVRNKHFVVLIVSNKLELRERISTLFVSLRNPGPPPPPPPEDPMLHRRF"
     gene            complement(14516..15499)
                     /locus_tag="PKW60_01435"
     CDS             complement(14516..15499)
                     /locus_tag="PKW60_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPJ98122.1"
                     /translation="MTGFLSQGEHVVGVDIGGTKLMAVVYDHKFKARGRNKRKSKSNK
                     GEPTEERLTRTIQAALDEAQVDTIRGIGVGSPGPLDPKTGVIIDTPNLAWKDFPLAQY
                     LKKAFKAPVVVDNDVNAGTYGEYRFGEVSDCSDILGIFPGTGIGAGIIIDRKIFYGYS
                     GAAGEFGHMTMEVGGPYCGCGKRGCLEAIASRVAIAADVAALAARGDAPFILANCGTD
                     LSKIRSGVLAEAVKAGDKKVENVIRKAAYYVGVATGNAINMLSPQAVVLGGGLVEAMP
                     ELYLEEVKRGVKEHAMPFLRRNVRIVTAKLGDDAVALGAASLLAERLGEFE"
BASE COUNT         3128 a         4618 c         4793 g         2905 t
ORIGIN      
        1 cgtgatggcc agccgagcgg atcaggatat caaatactgg cgggcagtcc agggcgaact
       61 ccagcggatg ctgaacgaat ttaccttcgt gcaccgctcc ctggtgtaca tccaccgcga
      121 agaggaaagc ctgttctccg acgagcagcg tcccaacaaa gccgctgtca cgctcgatgt
      181 atcgcgcccc ctgtccaaaa atgagatcca ggcggtattg ggggtcgtga gctcgttcgg
      241 cggcgcaaat ttggccccgg agaacatcac cctggccacc accgacggca aaaccctcaa
      301 cctgccgtcc gaggatgaat tcacccagat ctccaaccgg aggctggatt ttctcaatga
      361 atacgaacgg cagcgcgaat tgaaggccga aaccgccctc gccaaaatgg gcgtaaaagg
      421 ttttgttcgt gtaagtctcg atatcaacag cagttccgtc gaggagacag cgacaaaaac
      481 cgaaaagggc accccggtga gcgaaatgct gacccggact gaaaccacca gccgggagtc
      541 gttgcctgag ggcccgcccg gaaccctcgc caatattccg gaaggcgccc ccggtgcgct
      601 cgaaacgacg gaaacccagc gggaagagat atttaattac gagcccagca tcacgaaaac
      661 caccaccatc acgcctccag gggccgttct caaggcccgg gccgtggcgg ttgtgtcagg
      721 tgcgtaccag cctgttattg gtgccgacgg aaaacccacc ggcgacacgg agtacattcc
      781 ccgaaccagc gagcagctcg aggcctaccg cacagtggtt gcgacagccg tggggcccga
      841 attgaccccg gaagaagtct ccgtattcga tcatccgttt gaaatggagc aacatgcggc
      901 gattgaggtg tttaaggcgt ttgagtgggc gcaaatacag gaaaatatga taaaatgggg
      961 aagcaccgcg cttaaagtgg tgctggtgct tgtggggttc ctggtattca ggcattactt
     1021 gatgcgggtg ctggtatttt ctgaggggga ggaagaagtt ttgcctcctc aacacgagcc
     1081 caccttgcgg gaaatgcgcc gccgggagat ggctgaagag gtggaacgcg tgtccaccga
     1141 gcagccggag caggtggcat ccctcctgcg ggcatggctg gccgagggag aggagtaagt
     1201 cacggctaaa aagaagaaag cgccggcggt tgatttgagc gccttggatg gcaacaccaa
     1261 ggcggcgatt ttgcttgcct gcctgggacc cacggccgct tcccgcgtgc tgtcgcgcat
     1321 ggatgattct gaaatcgagc gcctcacact cgatttatcc agcctgggca ccattgcccc
     1381 ggagactcgc gaggcaattg tcgaggaatt tcaccagatg atggttgcca accgctatgt
     1441 cacccagggg ggcgtggagt tcgccaaaaa catcctggaa tccgcgctgg gcccggaacg
     1501 ggctgtggaa atcctggcgc gtctccagag cagcctccag gaagtgccgt ttgagtttct
     1561 gaagaaagcg gatcccagcc aggtgatctc gttcattcaa gatgagcatc cgcagacgat
     1621 tgccctgatc ctggcccatc tccagcccga tatcgcggcg accgtgctct cgtccctgaa
     1681 ttccgacgtg cgcaccgacg tcgtcttacg tatcgcaacg atggaccgga ctcctccgga
     1741 gatcgtgcgt gaggtggagc gcgtgctcga acgcaagatg gcctccgtct tcagccaggg
     1801 gttcaccttt gccggcggcg tcaaggaagt cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnntggcctc cgtcttcagc caggggttca cctttgccgg cggcgtcaag
     1981 gaagtcgccg agatcctcaa ccgcatcgag cgggccaccg aaaagaacat catggccgag
     2041 ctcgaggaac gcgacccgga actggccgat gagatctccc gcctgatgtt caccttcgac
     2101 gaccttattt acgtggatga cggcgggatc cagaaagccc tgcgcgagat tgaccagaaa
     2161 gacctggccc tcgcgctcaa acacgtggac gacgaagtca aagaaaagat tctcaagaac
     2221 atgtcgacgc gcgcgcgcga catgatcgcc gaggaaatgg agtacatggg gccggtgcgg
     2281 ttgcgcgacg tcgaggaggc ccagcagaag atggtgggtg tcgtgcggcg ccttgaagag
     2341 gcgggagaga tcattatcga gggacgaggc ggaagcgcgg aagacgacat cattgtgtaa
     2401 cggcatgcct ttgacagtgg ggagctgtaa tcggagacat ggcaaaaatc attaaatcgt
     2461 gggacatgag cgcgcaggaa tacccgtcgt accgccgcac ggcgctggag acgctgaaga
     2521 cggagctgca ccggagttca ggaacgcttg cgcttgaccc gcggcagata ctcgaggatg
     2581 cccggcacga ggccgagttg aaggtgcggg aggcttacgc tgaaggtcta cgccgaggca
     2641 cggaggcggg gcaggcgaaa ttcgccgagg cggtcggcaa agcggctgaa gcgctggaat
     2701 ccgcggctct ggcgatgcgc gaggcgcagg cggagttcct gctttccctc gagccgcagg
     2761 tggtggcgct ggccggagcc attgcggaac gcattctgcg ccgggaaccg tcgatcgagc
     2821 ccgaacgtgt gcggctcacc gcgcacgaag cgctggcgca cctggaaaag cgggagcgaa
     2881 tcaccctccg gatgaacccc gccgatctcg aggccctgaa ggaacagaac atcagcttgc
     2941 tcgaggaatt tgacgctatc ggtgacttgg aaatcattgc cgacgacagc gtcactccgg
     3001 gcggatgcgt ggcccaatcg gaactgctcg aggtggacgc gcgcatggaa tcccagtttc
     3061 agaagatcgt agatgctctg ctggagtaag cgtggcgatg cttgacctgg aaccctatca
     3121 gcgccgtgtc cgtcaaggca gccccgtgag cgtgtatggc cgcgttgtcg atgtcgtcgg
     3181 cctaaccgtt gaggcgaccg gcccgtcgat gcgcatcggc gacctgtgtt acgtccagcc
     3241 ccgctgcgag ggcgaccggg tgcccgttga ggttgtcgga tttcgcggca agcgtatttt
     3301 gttgatgccc ctgggcgaca tgcacggcat cgcgcccgac agcctcttga tccctaccag
     3361 caaaccgcag accgtccggg tcggcgacgg gctcctggga cgcgtgatcg acagtatggg
     3421 cgtgccgatc gatggcggcc ccgaccccaa aacggtcgcg gaggtggcgc tcacggcctc
     3481 gccgccgcgc cccctggaac gcatgcgtat cgagaagccg gtcgccacgg gcatccgggc
     3541 tattgacgcc tgcatcacct gcggtaaagg ccagcgcatg ggtatcatgt cgggcagcgg
     3601 catcggcaaa agcaagatga tcggcatgat tgcccgcaac accaacgccg acgtcaatgt
     3661 gatcgcgttg atcggcgagc ggggacgcga ggtccgcgag tttgtcgaga acgacctggg
     3721 ccccgaagga ttggcccgct cggttgtggt cacggcgacc tccgacgagc ccgcgctgcg
     3781 gcgcgtcaaa ggggcctaca tggccacggc catcgccgaa tggttccggg aacgtggcgc
     3841 cgatgtcatg ctgatggnnn nnnnnnngcg cagcgggagg tcggtctggc catcggcgaa
     3901 ccgcccacca cgaaaggcta tccgccgtcg gtctattcgc ttctgcccaa actgctcgaa
     3961 cgcgcaggaa cctccgcgaa tggcagcatt accggcatct acgcggtcct ggtcgaagcc
     4021 gacgacctca acgatccggt gggcgatgcg gtgcgcagca ttctggacgg ccatatcgcc
     4081 ttgtcgcggg acctggcgac gcgcaatcat tatccggcga tcgacgtcaa cgagagcgtc
     4141 agccgctgca tgattgacat caccagcgaa gagcaccggc gcctggcggg cgagctccgg
     4201 cacattctgg ccacctaccg ggatgccgaa gacctggtca atattggcgc gtatgtcgcc
     4261 gggagcaacc cggacatcga tcgggcgctt cggctcatgc ctcaggtgcg ggcatttctg
     4321 cgccagggat tgtatgaaga gtcgccgttc acggagatcg agcgcctgat gcgaaacgcg
     4381 ctccagggat aggagattca tggccccagg aaaaccattc cgttatgaca cgctcctgcg
     4441 cgtgcgccag cggcaggaag aactgaaatc gatggccctc gccgagatgc ggcgggcggt
     4501 agacttcgcg gagcagcagc gcgcggagat cgtcgagcag cagttcgccg cgctgaatgc
     4561 cgcgggcgaa ctggcgcagc gtcagttcaa tgccagcgag gtccgccgtt acttctccta
     4621 cgaacgccac ctggcgcggc ttgccgttga gaaggatgct gagatccggc accttcgtac
     4681 ccaggaacgc ggccgccgga aagagctcga ggctgccatg aaacgccgca agatgcttga
     4741 acgtctgaaa gagcgcctcg aggaggccta cgactacgtc gtgaacagag aagagagaaa
     4801 acgcctggat gagacggccg taaaccgggc cgctctggcg gatggaacgc aaaagccatg
     4861 aaagcactgg tcactgctgt tgtgtttgtg gcgacctttg tcgcgaccgt gttgctgctg
     4921 ctccttttca cgggaaacct caaccgggaa ggcctggcca ggctgcgaag aggcggagaa
     4981 ggcgcggccc cgcccgcgcc tgttgtccgg gccgcggatg agtcagacgc gctgctgaac
     5041 gcgctgcgca ctaaagagca gcaactggcc gagcgcgaag cggccattca ggaagaggaa
     5101 aaacgcctgg cgcaggtgag gagggaactc cagacgctgc gcgacagcct ggagacgatg
     5161 cagaaaaagg tcaatgaatc gatggacagc gcggatgcgg acgcccgcac gcgcatggag
     5221 gcggtggccc ggtcgctggc gagcatggaa gccaagaacg ccgcgcaaac gctgacctcg
     5281 gagatgttca cgcccgacga ggccgcgacg attctgcagt gcatcgaaga agaccgcaaa
     5341 cgcggtgaaa tactgaatga aatgcagccg aacgaggcgg ccacggtctt acaggccctg
     5401 aaaaggcact ccttcggctc ctgaggcgca tgccggccgg cgctggccgc ggcctcttcg
     5461 tgccccgtct cttcgtgacc attcctcagg cgcggagtcc ctctgcgccg acgcccttcc
     5521 agaatcttcg cccgtgtgca tgctgatttg acaattgtgc gcccgggcgg agttctttct
     5581 cggtttcctc cccgtatttt tgaatacgta caggggagga atgcctattt tcaaaagaaa
     5641 ggaggtgaat cgatgtggaa acatcacccg tattgctgtc gttgattccg accggacagg
     5701 gtatcgaggg cggcttggat gctggctggc aggaaggcag cggcgtttcc ggcgagagcg
     5761 cgtcatttgc gctcgttctg gcgcagcatg ctgcatccaa catcgctgcg acgctgggcg
     5821 atccggcact ggcccttccg agcgaagacc ttcagctgga aagcggccaa ccaagggctc
     5881 cttcgggggc cggcctgctg gaaagtaccc agccgaggtc tccttcggat actgcgcggt
     5941 cggaatgcga tcagccaggg cctcccacag gagcggtcct ggtggaatgc gccccgccgc
     6001 aaactctctc ggaagttgca gcagttccgc cacttctctc gcaggcgaac tacgtgctta
     6061 gagacgtgga agctacggaa tccggtgaaa cggaggcggc cgccaaagag gaggatcacg
     6121 atgccggcgc gctgctggtt ccgccgttcc cggaacaagt ccctggccag gacggtgcgg
     6181 tcacacgcct gagcgatgga cattcggtgg aaagcgaccc aacggagtct ccggtgactc
     6241 ttcgaagcac ggccacgtat ggcgaatcgc catccgccgc gtggggaaga gccgggggac
     6301 aggaaggcca agccgtggat gcgcgcacgc tgcgtggaga ggcaaaatct ccgtttgtcc
     6361 tgcgccttgc gccgccaaaa ggctttgacc cgcctgtgta cgacacgggc gggggtccgc
     6421 ctgtgctctc tgcgcctgcc accgtcgtgt tcgggctcca acccgcgcgt gaggcctttg
     6481 tggaggcagc tccggagctt ccatctgccg gtacagccgc gccgaggacg ccgccggtcg
     6541 aacaggagca gattgcgagg cttccagagc agcccagcga cctcgaaccc gggttccccg
     6601 acccaaaaaa cgcgatggaa tcccggccga gggtcctgga ggagtccctg gtgcagctga
     6661 gcacctcgga gacagttgag tctgacatct cagacgcacc gcccgtgcag gatgcaccat
     6721 cctcagccga gccggcatcc gcgaaccact cttctgcagg cgcgggcccg gttgccatcc
     6781 cggcgggcgc atcacaagcg gccggcccaa caccggcggg cgtcgaagtg gtggagttcc
     6841 atgcatcgcc ggaaccggct ctgcgggatt ctctggccga gctggccgtc aagagcgtcc
     6901 gctacctggt gtcagaagac aaacgctcgc ttcggatccg gctcgtgccc gaatcgttgg
     6961 gcgaggtgcg tattgagctg gtgtcaacgc gcaacgagct gcatctgaaa ctggtttcgg
     7021 gcagcgcgtc cgtgcgggag gcgatggaga gcgcgtccga tgcactccgg cacgccttga
     7081 cgaaagacgg agtccatctg gttcgcgtgt ctgtggccgg agacggcgcc gtctcgtccg
     7141 ggcagggcgg ttttccggaa cggggtgcat gggccaatga ctcgccgggg catcggtatc
     7201 cgggctcagc ggcccggtct tatggaaatc cggaagcgca tcgctcaggt ccagcggtac
     7261 gcgcggcgta tcacgacgga agactgagcg tgtacgcctg acccgcggag ctctacaggc
     7321 tgaggccgtg tggagataac cgcaagatga ggaggaaagg agggacatat gaatgccctt
     7381 gtagccgttc gcggcaattt ctcgggcgat ttgagcccga atccggtgga tcgggccaag
     7441 gcagccacag aattgcagtc gatggagaag gcgcgcctga cgcatgtgct caaacaagcc
     7501 ggactggtgt tgaaaagcaa aggcgccgag gaggcgcacg cgatgaaagg caaccttgat
     7561 cgcgacgctt ttcttcgcct gctggtcctg gagatgcaga atcaggatcc cctgaacccg
     7621 gtggacaaca ccgagatgat cgcccaactg gctcagttct cgtcgctgga gcagatgaac
     7681 aacctcaatg agagttttga agccctcagc ggcaatattg accagttaaa tttcatttcg
     7741 gcgagtgggc tcgtgggaca cgaagtgacc gggattgatc aggacggcaa tgtcgtgcag
     7801 gggacggttc aaaccgtgca cctcgaccag agcctcgtct acctcaacgt ggatgggaaa
     7861 ctgatctcca tggccggcgt tctgagcgtg ggacacgcgc catgatcaac ccggtacaag
     7921 cagcaaacat gccggaaagg ctcgccgcgg ggcgcctgtc tcaggcttcg gcgactctgc
     7981 cgcagacgcc cttctccgat gtccttgccc aggcgcagcg tacgcagtcc gcgctgcgat
     8041 tttccgcgca cgccgcacgc cggcttcgag accggaatat cgctttgtcc gaagccgacg
     8101 agacagcctt gcgcgaaagc gttgaccgcg cagccgccaa aggctcgcgg gagaccttac
     8161 tcctgaccga aaacgcggcg tttgtggtga gcgtcgccaa tcggaccgtc atcacggccg
     8221 tttcccggtc ggaaatggca gatgccgtat tcaccaacat tgacagtgcc gtagtcgtgg
     8281 cccccggccc ggctcgcagt tgaaaacacg aagctcttgg gccggaccct gttcaggggg
     8341 gccctggcgc cgccgactga acgacgcggc gcgctgacag gagggttcag acaatgggta
     8401 cagctatcta cacgggcgtc acgggcctgc aaacgcacca gcgccgcatg gacgtgatcg
     8461 ccgccaacat tgctaatgtc aacacgccgg gataccgtgc ggcacgtgcg ctcttccgcg
     8521 acctgttttc acagacgctg gaaggcgggc gcggtccggt agacggattc ggtggctcca
     8581 atccggtgca gatcggcctg ggagtccaac tgggaaccgt cgacaccatc cacacgcagg
     8641 gttctttgca gaccaccggc aacaactcgg atttggccat tcagggcaca ggatttttcg
     8701 tcctgagcaa cgggatcagc cagtatttta cccgtgacgg gtcgtttaca ctgaacgccc
     8761 tggggcagct aatcgatccc gctaccggta tgcgcgttca gggatatctg gccgatgaga
     8821 ccggcgaggt caatgtgagt gccatcccca ccgacttgga ggtccccgtg ggcggcacgt
     8881 ccattgtccg ggcaaccgaa caagtgatgg ttcggggcaa cctggactcc cgggccgtcg
     8941 cgggcacggc cgtacggcgc cgggtcagcg tgttcgattc gcttggcacg cctcgggagt
     9001 tgatgacgac tttccggaag attccccagg taggcgtagg cgggaccaat tacaatgcgt
     9061 ggtcgtatat cgtcgagtac accaacacgg attcgccccc ggtgactact caggtcggcg
     9121 aaggcgttgt cctgttcgat gtcgaaggca actattttga tgaaggcacc ctcgtcggcg
     9181 gcgttttcac cacacggccc gcgggaacac ccgaagtgtc ggtgccgctg gcagccttcg
     9241 gcagtctcga ctcgtacccg gatactcctt tcgagtttga gatcaatttc tcgtcggtca
     9301 ccgaactggc gtcggatagc gatgcgacgg tcaccaacca ggacgggttc gcccgcggca
     9361 cgctcgaaag ctttaacatt gcccgcgacg gcacgatcaa tggcgtgttc tccaatggac
     9421 ttacccgcgt cattggccag atcgcgctgg cctcgtttgc caacgtcggc ggactggagc
     9481 gggatggaag caacctgttc cgggacaccc ccgcgtcggg cacgccgcaa atcgggctgc
     9541 ccaataccgg ggggcgcggc caggtatccg gcggcgtact ggaaggctcc aacgtggatc
     9601 tgggcactga attcagcaac atgatcgtca cccagcgcgg atttcaggcc aatgcccgca
     9661 ccattaccac cgcggacacg ctgctccagg aaaccgtcaa tctggtccga tgagccgtaa
     9721 tgtggctcct ccgtgaaggc atccgggggc ggttcgcgcg gctgggccgt gcgcgccgcc
     9781 cccgattttg cagcgtctgc cgaaaaaccg ggcatgaccc cccgcaacgg gctggtttca
     9841 ggttgtgggt tgacactcag agtgttagac tattgcagca tctgcagaac gcattttgag
     9901 aggggagctc aacaaaaggc gtagcgtatg gatattgcca cactggtggg tctgatttcc
     9961 ggggtcaccc tggtagtcgt ctcaattctc atggggggaa atccaggcgt cttctggagc
    10021 tcgtcgtcgg tcgtgattgt catgggaggt accctggcgg cgaccctcat caattttccc
    10081 ctgggcgacg tgttgagcgt gatgaacacg gtgaagaacg ccttctttca caaggatgtt
    10141 ccgcccgaga gcctcatccc gcggcttgtc agctttgcca ccatcgcgcg ccgcgaaggc
    10201 atccttgcgc tcgaatccca cgccagcgaa gcggaagacg agttcctgga gaaaagcgtg
    10261 cagctcgcca tcgatggcac tgctccagag ctcatcaaag acatcctcac gaccgaactg
    10321 gccttcatgg aagatcgcca tgccatgggg cagtctatcc tggtcgccat gggtacttat
    10381 gcaccggcgt tcgggatgat cggcacactg atcggcctcg tacagatgct tgccactctc
    10441 gacgacccgt ccaaaatcgg cgaggggatg gcggttgcac tcttaaccac cttgtatggc
    10501 gcccttctgg ccaacatggt ctttctgccg ggggccggga aactcaaagt ccgcaccagc
    10561 aacgaactgc tggccaagga attggtgatc gagggcattt tgtccattca gtcgggcgac
    10621 aatccccggg ttgtcgaaca aaagctgaag gcattcgttt caccggccgt tcgccggcag
    10681 gtcagcgcag tgtaatatgc ccaaaggaaa acgcaacaaa ggaggcgaag agcagccggg
    10741 cgcgccaggc tggatggtga cctatggcga catgatgagc ctcttgctct gtttttttgt
    10801 actgctgctg tccttttcgt ctgtcagcga aaaaaagtac agtcaggcca ttcactcgtt
    10861 gaaaggcgca ctgggtgtgc tccccagaaa tctctcggtc attcaacctc tgcccgtgga
    10921 gcagatggcg cccaagcgcc agcgtaccat cgaacgcata ggccgtatga tccaggaacg
    10981 cctgctcatc aacaacaagg ccgaggacat caaaatcgag tttgatgaag aaggcggcct
    11041 gaaaatcaat ctgccgagcc ggattttgtt cgacaccgcg agggccgagc tcaaacccga
    11101 tgcgtttccc gtgcttgagg atatcggtat ggttttgaaa gatctcccgg gtgccagcat
    11161 cgagatccgc gggcataccg atatccgccc cttgaccccg ggtggaaaat tcgaagacaa
    11221 ttatcatttg agctatggcc gggcgaagag cgtcatggat ttcctccatg agcgtgctca
    11281 gattccactg ggcgtcttcg aggtgatcgc ctgtggacca tcgcagccca ttgctaccaa
    11341 tgagacggaa gaagggatgc aagccaaccg gcgggtcgag atcaatgtcc gcggcgactt
    11401 ttctgaagag acgacgagcg aactacggaa aagcatcgac caaatgcaaa gcggcatcgg
    11461 tctgaagccg gaacaataag cgagggacgt gtttatggct gatgaatcac aacccgcggt
    11521 acccgcgccg aaaaaatcct cttttatcgg cctgctcatc tggttgagcc tggcgttcat
    11581 ggttgcggtg ctcggcgctc tggcacttta ttttgtgatt gtccgccccc ggctgacgga
    11641 ggaagcgctg cctgcggatg cggcttccgc ggacaagctg aatcctgact ccgttacggt
    11701 ggacttcgag gaatccgtgg cctcggtaaa aatgcccgaa gacagccagc tcccggcatc
    11761 gctgctgtcg tttcaggtat cgcttgagtg caatgatccg gccacggcag ccctcatagg
    11821 gaaacacatg gcgcgcttta cggatatgat caacaagcag cacgaattcc ggacgcgggc
    11881 ggaactcgac gatccgcgtg tcaagaccga aatccagaag acgattctac tcgaggccaa
    11941 tacaatcctg aaacaactcc aaccgggccc gcccaacccg aatatgcggg ttacggcggt
    12001 ctttcacaaa cggttcctgg tgagcgattg aacggaatgg aagcagaagc caagcagcct
    12061 catgagcacg agtttcgcga ggtgaacccc gggggcgtct acgggacaga acgcgtgtca
    12121 ctggacgacc tcagagaggt caagatgacc cttaccgcgc accttggcta ttgctcgatg
    12181 acggttcgcg aggtcctggg gcttcggcgc ggttcggtcg tttccctgga tcggcaggcc
    12241 ggggaaatga cggacatcta tgtcaatgga ctgccacttg cccggggcga ggtggtggtt
    12301 attgcggacg tgcttcacgt ccgtatcggg gagattattg gtcaggaaga tcgttcaggg
    12361 ctggaagaag cgcatgaaga cgcagcaagc aagtagtggc gcgcgcctga tcctggcagc
    12421 cgcgcttgtc ctgggatgcg tgtggggagt ctgtgcccag gatccggcct cggaacccgt
    12481 ggaaaccctg gttcagcctg aggacaacaa cgagtatgag gcaacgctgc ccccgcaatt
    12541 ggatctccgt tcggatttga cgccaacggt ccctccggag cccgttcccg agattccagc
    12601 cgtggtggag cctgcagagc cgccggttga accctcagcg gaaagccccg acccgtttat
    12661 ggagcaagtg aaccgggaac tggctgcccg gcgcggcaca gcgcccgatt ccgccggcgc
    12721 gaacgaacca gcggccttcc ccgtgactga gaggcgtccg cgcagccttc tccgggcgtt
    12781 tgcgtggctt ctggtcgtga ttgccctcat tctgctggtc tattacgttc tccagcggcg
    12841 cggacgtggc ggtccactgc ggctcgggag ccagctgggc accgttctgg ggcgaatcta
    12901 tctcagccct cgcatgtgcc tccacttcgt ccgcactgga ggcaaagtcc tcgtcgttgg
    12961 gcagacgcaa aacgccttga ccttgatcgc ggatttcaat gaagctgaat tcgcagtccc
    13021 ggtggaatcc gcagcgcaag gtgcagctgg cgaagcgccg gccgaaccca aagccgactt
    13081 cctctcgctt ctccgcgacg gtatctcccg aatctatcga accggcgtcg agcatgatca
    13141 ggcgtctgaa ccagtggaac cggtaggcga ggacgatatc gccgcattgc ggggggacat
    13201 ccaccggttg caggaatacc tgcgggactc tatgcgtgaa cccgaagcct aagctgctgc
    13261 tgatggccct ggccggcggg gccgcgttgc tggtttccgc gggcgctctc gcacaggagg
    13321 cgcccggcgg gaccggtcca ctgggtatcg agaacgtctt ccggggcctg gaaggcgccg
    13381 cggccccgga ccgtatttcc accacgctgg ccctgtttat tctcctgaca gttctctcgc
    13441 tggccccggc catagcggtc atgacgacct cgttcctgcg catcgtcgtc gtattcggct
    13501 tcctgcgcca ggccatggcc acacagcaga cccccccaaa ccaggtgctg attggactgg
    13561 cgctgattct cacgctgttc gtcatgtcgc cggtataccg gcaggtctac gatcgggcgt
    13621 accggccgta tgtcgaggaa gacattggat ggcaggaggc cgtggacaat accattggtc
    13681 caatgcgtga tttcatgttc cgccaggtac gcgccaaaga ccttgccctg ttcatcgaca
    13741 tttccaaaat ggagcgcccg cgcacccgcg acgacgtgcc cacgcttacg ctggttgccg
    13801 gctttgtcct gagcgagatc cgcattgcgt ttcagatcgg tttcatcatc tatatcccct
    13861 tcctgatcat cgacatggtg gttgccagca ccttgatgtc gatggggatg atgatgctgc
    13921 cgccggtgtt cgtatctctt cccttcaaga tcatcatgtt cgtcctggcc gatggctggt
    13981 atctgctgat tcaggaactg gtagcagggt tcaagtaggc agggcatgga agatggacag
    14041 gaaactggcc gccattattg caggcatcgt atttctcgca atccttcccc tggtgtgcca
    14101 ggtgacgaaa gacccggttc caattacagt ggaagacatc aaagccgcgt tccgagagcg
    14161 caacttttcg atcgcgaacg aacatacggt taaccgtccg cgttaccagg ccgacgcgga
    14221 gatcttcatg accatcaatg gcgccccggt gtatttgttc acgtattcca atgtgactgt
    14281 cattgaacgg gaaatgaaga atatggaagc ggatgcaaaa cggctcccgg cggctctggg
    14341 ctcggggaat gcggtcaaag ccgttcgcaa caagcatttc gtcgtgctca ttgtgtcgaa
    14401 taagctggag cttcgcgagc gcatcagcac cctgtttgtg tctctccgaa acccgggtcc
    14461 accgccgccg ccgcctccgg aagatccgat gctgcatcga cgcttctgaa gccgcctatt
    14521 cgaattctcc caaacgttcg gcgagcaggc tggccgcgcc cagcgcaacc gcgtcatcac
    14581 caagcttcgc ggtgacgatg cggacgttcc ggcgaaggaa gggcatggcg tgctctttga
    14641 ccccgcgctt cacctcctcc agatacagtt ccggcatggc ttccaccagg ccgcccccga
    14701 gcactaccgc ttggggactc agcatgttga tggcattccc cgtggcgact ccgacgtaat
    14761 aggccgcttt tcgtattacg ttttcgacct ttttatcgcc cgccttcacc gcctccgcga
    14821 gcactccact gcggatcttc gagaggtccg ttccgcagtt cgccaggatg aatggtgcat
    14881 cgccccgggc cgcaagcgcc gccacatctg cggcgatcgc cacccgggag gcaatcgcct
    14941 cgagacagcc ccgcttaccg cagccgcagt acgggccacc cacttccatg gtcatgtggc
    15001 caaattcacc cgccgccccc gaatagccgt agaagatctt cctgtcgatg atgatccccg
    15061 ccccgatccc ggttcccggg aatatcccca agatgtctga gcagtcgctg acttcgccaa
    15121 accggtactc gccataggta ccggcgttca cgtcgttgtc caccacgacc ggcgccttga
    15181 atgccttctt caaatactga gccagtggga agtccttcca ggccaggttg ggcgtgtcga
    15241 tgatcacccc ggttttcggg tcaagcggcc cgggcgaacc tacgccgatt ccacggatcg
    15301 tgtccacctg cgcctcgtcg agcgccgcct gaattgtccg ggtcagccgt tcctccgtcg
    15361 gttcgccttt attggatttg cttttgcgct tatttcgccc cctggccttg aatttgtggt
    15421 catatacgac cgccatgagc ttcgttccgc cgatgtcgac accgacgaca tgctctccct
    15481 gcgacaagaa tcccgtcatg agcgttctcc attggttatg agatgaagga tagcacgtca
    15541 gcgttcggcc gtta
//
LOCUS       DAOPBQ010000019        15124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_3982_length_15124_cov_4.217665, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000019 DAOPBQ010000000
VERSION     DAOPBQ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 15124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15124
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3982_length_15124_cov_4.217665"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..836
                     /gene="thiD"
                     /locus_tag="PKW60_01440"
     CDS             18..836
                     /gene="thiD"
                     /locus_tag="PKW60_01440"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012459419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HPJ98123.1"
                     /translation="MPDTTSDNTAIPCVLTIAGSDSGGGAGIQADLKTFTALRVYGMS
                     AITSVTAQNTVGVTGVHDLPPEFVVEQIDDVARDIGVDAAKTGMLSSAGIVEAVADCV
                     ARNSITKLVVDPVMVAKSGDALLHESARQALCSQILPLAWVVTPNVPEAEVLAGVRIT
                     GPPSIEKAAAIIHRLGARYVLIKGGHMHGKDAVDYLYDGQGVQTFSAPRIATKNTHGT
                     GCTYSAAIAAYLARGCATSEAVQRAKQYLTGAIQHSFPLGAGHGPLNHFWRTGA"
     gene            complement(879..1922)
                     /locus_tag="PKW60_01445"
     CDS             complement(879..1922)
                     /locus_tag="PKW60_01445"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HPJ98124.1"
                     /translation="MSGLADAFAYQDRILQGVSRTFALTIPQLPPALRRVVGNAYLLC
                     RIADTIEDSESLSIAEKRRYYDWFVQALDERGSGAEFGQDLAPKLDGGTLDAERDLVR
                     NTECVLRITHSFSADEQRALQRCIRIMSDGMEEFQEGQFTDGLRDEAHLDAYCYHVAG
                     VVGEMLTHLFCAHSEAVAARRNELESRAVSFGQGLQMTNILKDIWEDRQRHVCWLPQA
                     TFERAGFDLRHLETAPGNPAFQEALSHLIGVARRHLEVALDYTLLIPKSEPGIRAFCL
                     WAIGMAVLTLRNINRRRDFTSGADVKISRRSVRATICLTRLFGWSNRLLRLAFRLAAA
                     GLPRPAPAAPAPS"
     gene            complement(2039..3067)
                     /locus_tag="PKW60_01450"
     CDS             complement(2039..3067)
                     /locus_tag="PKW60_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPJ98125.1"
                     /translation="MNTSDQVERQQPALQGQEALARVIREFYDVGFVDMPVPVPMAHQ
                     RRHRKLIVMTGAGKFLIKTYQRDPYVLDALRFQHRLSQHLDENALPVARILPAKTGRK
                     IVEGDNWALELQEFVDGTPMEVTSETLAISAHALGQLHDVCRDFPRPERDVRMWRFSE
                     VPRTLFAQFFEMAKEGGNEAKVMDECNQIALFLRDASQELSYEARNQFETGLIHGDWH
                     GGNLIFKDGRLQAIVDLEFAGDGCFLEDLAYGISNLCVRTTMRPERLGKRTDIMLKHY
                     QKHRNLSPYEQVALYYAVGVKHVATVSYQALQSKGVVAGYSAQSWMERLAVQCRWLWD
                     RAHGVRWG"
     gene            complement(3064..5274)
                     /locus_tag="PKW60_01455"
     CDS             complement(3064..5274)
                     /locus_tag="PKW60_01455"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPJ98126.1"
                     /translation="MRTMYSRLGLVLMLVAVIAAGCREPQQPVTQAAPEHLPATALPP
                     EEVAGPDDSAPGEAPAAAQATVYRTKWGIPHIYSDSLEAAMYAFGYAEAEDRLEDIFQ
                     NARTALGRMSEIFGKGHIEDDYIHRLFRNPEVSQRAYTESPDRVRLICDAFAAGINAY
                     VADHPERTPDYAFEFEPWHCIAIGRAMILQWPLGTVYGDLKNRKEDPGRGSNGWAIAP
                     SRTADHCAILLTDPHLTWRGIQVFYEARLKAEEVDINGHFVVGAPMMGLGHTAHLGWA
                     CTTGGPDTSDVYELKINPDNPLQYELDGVWKDAETRVIEFDVKDGEPFQRTAVYTEWG
                     PCVEGPDNGVAYAGATPYMDSVGLLEQVYRMSTANSCDEFYEALAMNEFMAQNLLFAD
                     TQGNIQYVRTGRTPIRPEGYDWSAPVPGYTSATKWLGITGIADLVQIKNPPSGYMQNC
                     NISPTLMFPDSPMTPDKYPDYIFNVSWDLMNMRGRRALQLLGADDSVTEEDAIAIAMN
                     VYDLLAEPWQKALKAAVAAAGQEYLADADLQAALDSALSWNGYVRPESKGAPIMKAWR
                     LLCENAVDVETIARGEEPSPADQVKLADLFMKTVTDMKAMYGTVDPMWGDIHLLGRGD
                     TFVGCGGVDFGGGPNKSNKTETLRDVGYAESKEMPGKFVARKGSVAMMLMFMRPDGVK
                     SFSVVPWGNSNDPASPHYMDQGRELYSQLKFKETLFKKEELLKNVQSEKVIALP"
     gene            5604..6320
                     /locus_tag="PKW60_01460"
     CDS             5604..6320
                     /locus_tag="PKW60_01460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPJ98127.1"
                     /translation="MTHIHRFFVEAGDVQGDVAIVAGPEAHHALHVVRVQHGQPVCLF
                     DGLGRELQGLVREASRHDVTIDITDERTVQPEIRTVTLLQASLRRDKAVEELIRRCTE
                     VGVHRFVFFPAERSEHPPRISEKWRRWAIESCKQCGRLWLPEFSLAPGLEAALAGTWS
                     ALLIATADEAPVPLRSAVQGDCVALIVGPEGDFTPAERSLALACQAHPISLGAATFRA
                     EVAATLAAALIRYEFGALGP"
     gene            6458..6805
                     /locus_tag="PKW60_01465"
     CDS             6458..6805
                     /locus_tag="PKW60_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPJ98128.1"
                     /translation="MAEETIFGKILSGEIPSQEVYSDEEFYAFRDVNPAAPVHILIIP
                     RKPIPKVTHLTEADAALMGRMFIAANKIAEQEGLAENGFRYVINCGEWGGQTVFHLHL
                     HILGGRAMTWPPG"
     gene            6820..7680
                     /locus_tag="PKW60_01470"
     CDS             6820..7680
                     /locus_tag="PKW60_01470"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPJ98129.1"
                     /translation="MRIDAHTRLCAVIGNPVGHSLSPLLHNAAFRAAGLNYIYAAFRV
                     EDVAACLAGMRAMHGFRGMSVTIPHKLAVMSCLDEIDPMARRVGSVNTITNENGRLTG
                     STTDGPGTLRAFQEAGISLDGKRVLFIGAGGAVRAVAFAVAELTRAEAITVIGRNAER
                     VRALTEDVAAQTGRPIEAAGLPAGLAGAVAESDVLIQGTPVGMHPNPGQSPVPREWLQ
                     PRHICFDMVYRPNKTQFILDAEAAGCTTLLGIEMLLNQAALQFELWTGAPAPLEAMRN
                     AARAAFNEPP"
     gene            7779..8798
                     /locus_tag="PKW60_01475"
     CDS             7779..8798
                     /locus_tag="PKW60_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ98130.1"
                     /translation="MKSPWTLRFIPFALAALLLAGNSANAEFPPLPEGAFSVVALPDT
                     QAYSAKAPEVFAAEMQWILDNIESESIVFVTHLGDIVDNNNIPEQWAVASQSLAMLHG
                     KIPYGLGVGNHDMKGSTGDSSNFQAAFPASLFLDFDWYGGQYRNNANSWQTFSAAGMD
                     FLIVHLECNAPDDVLAWADGVIAAHPDRRLIITTHMYLGPREKPVDKNDFFDAPKGRM
                     KWKKCHGDAGNTPQQMWDKCFSRHPNLIMVLAGDQSRTQAYYQTAIGVHGNVVHELMS
                     DYRDGYFRVYRFLPDENRVEVFTYSPVLKKLCDGTTIVPDPDRHQFSFFYAEEPALAE
                     AAPDK"
     gene            complement(9054..10625)
                     /locus_tag="PKW60_01480"
     CDS             complement(9054..10625)
                     /locus_tag="PKW60_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HPJ98131.1"
                     /translation="MRKSMFLVLAAVVVLAATPALAELQNVIVGGQVRIRGNYYTNTQ
                     TNINRSNLHWPAGLLSGRSIGTGSNYNWDGVRGAIGWDDKENSLGYVEQRTRLNVKAD
                     FSNEVAAFIELDSYDIWGEDFRSDYITGADSRAWTGDDVEIYQAYIEANEMFGLPLRL
                     RVGRQELSFGSEWLVGVNDTSSGFTGLSFDAVRLTYATDMFSVDAFMAQLFEGGAFEE
                     DEDIWLYGVYASYLGLEDIVIDAYWLWLRDARRLSDTDLTWFGEWWEDIFGIDDYDPT
                     NLHTVGLRGAGTIGAFDFEAEFAYQFGEADQVGYLFRPYIYGDDSADFSAWAGNLEVG
                     YTFDMAWQPRVWVGGAYFEGQDKRDISWWEWISPFSSPRASVSFNRLFSNWEYSQFLD
                     NTTLSNMWTVRGGVQAMPTESIELALIVKYFRALEEFDAPAHITLGGWRIPIAPFFSF
                     WTMQNDRDLGIETELKATYHYSEDLVFEAGWAHLFVDDGATDGTYVLFNGLGFSGGTS
                     DDDADYLYLETRIQF"
     gene            complement(11184..12518)
                     /locus_tag="PKW60_01485"
     CDS             complement(11184..12518)
                     /locus_tag="PKW60_01485"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPJ98132.1"
                     /translation="MPSEHEVLDSMRHIMDPDLGRDIVSCGFIKNLKIEGGHVSFTLE
                     LTTPACPMKSHFLESCKKAVIELPGVTSVDVTLSAQAPRRLQGQGAPGMTENLKNIET
                     VIAVSACKGGVGKSTVASHLAMAMAREGLAVGLLDTDIYGPSFPTLFNKHRPQVFGHD
                     NKIVPLNIHGLKVMSLGFVLGESPAVMRGPMVSNYTAQILLQTDWGKLDYLIIDMPPG
                     TGDVQLTIVQQAALDGAVIVSTPQALSLVDVAKGILMFEKVNVPVLGVVENMCSFTCD
                     GCGKVHYPFGRNQGMLQERFGLQTLAELPIMPGVSDLSSPDAGADLEVMAQLAENVHR
                     AVGKSRAGKEELPDVVEREYMVEFTWPDGFVAEVPNRMLRAACPCAVCVDEYTGEKLL
                     DPASIPDKIRFSRVNYLGNYAISFEWSDGHTTGIYSWDYLRELARKASAGAE"
     gene            complement(12857..14419)
                     /locus_tag="PKW60_01490"
     CDS             complement(12857..14419)
                     /locus_tag="PKW60_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPJ98133.1"
                     /translation="MAELFRRFVEALQRGRRFVTYDVWRIGKPGEQVPSGFLIKQIRV
                     AILLFRGLMSDVLWLRTSSLAFATILAIVPFLAFAFFIMQTFDLAQSLTDVLENSIHR
                     YAERMGAVRDAIPPSPPQEPREHAETMRTPRAVSSDPDERARRNRELLESALGWLLEG
                     VPPPEDGQDQLAAESPLKVILEMVEESGKSTAMGTFLGIVFVFFSVFGLMNGIESAFN
                     AIWGTKRNRSWFRVFTDYMLIVLFLPFVVAFVVGLTTALETENLAWGSLRLLLSVARY
                     LVLWLALATVYRVVPNTRVPARYALLGGIVAGTIWIFTAKAFLHFQWGVARANFFYGS
                     FALIPLLLVWIYTCWLIILFGAQITFAYQYEKTFALERLAEHASHAYKEALCLRSVIE
                     VSRRFDQGLPPLSAAECAAEWNVPVRLLNDGLDELEEAGLLRQVNSEPVRYQPARSID
                     KITVGAVLRAVREHGRDPSALRDDETMKRLLERLYEPGGGIFAMTIEEVIREFHLEPA
                     VTADSTEAPEMV"
     gene            complement(14463..14948)
                     /locus_tag="PKW60_01495"
     CDS             complement(14463..14948)
                     /locus_tag="PKW60_01495"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPJ98134.1"
                     /translation="MSPADTAARGPDASPAGSEALAVRAASPAEYPPDSVVIHVAKRD
                     HILSIQSGDALLARFPVGLGRNNATPEGEFLIANKVTDPDWSDRGRIVKAGDPENPLG
                     RHWLGLERDGRVTPYGIHPTNEPHSIGANMSRGCIRMRPADAEAVFQWCPVGTKVIIG
                     P"
BASE COUNT         2954 a         5010 c         4339 g         2821 t
ORIGIN      
        1 cgcaggagta ctcatctatg cctgacacta cctctgacaa caccgccatc ccctgtgtat
       61 tgaccatcgc cggcagcgac agcggcggcg gcgccggcat tcaggccgac ctgaaaacct
      121 tcaccgcgct gcgggtctac ggcatgtccg ccatcacctc ggtaaccgcg caaaataccg
      181 ttggcgtcac gggcgtacac gacctgccgc ccgaatttgt cgtggaacaa atcgatgacg
      241 tggctcgcga tatcggcgtc gacgcggcca aaaccggcat gctgtccagc gccggcatcg
      301 ttgaagccgt ggcggactgc gtggcgcgca acagcattac gaaacttgtg gtcgaccccg
      361 tgatggtagc caagagcgga gatgcgctgc tgcacgaatc ggcgcggcag gccctgtgct
      421 cgcagatatt gcccctggcc tgggtggtca cgccgaacgt acccgaagcc gaggtcctgg
      481 ctggcgtacg catcaccgga ccgccgtcga ttgaaaaggc cgctgcgatc attcaccgcc
      541 tgggcgcccg gtatgtgttg atcaagggcg gccacatgca tgggaaggac gcggtcgatt
      601 atctgtacga cggccagggc gtccagacct tttccgcccc ccgaatcgcc acgaaaaaca
      661 cccacggaac cgggtgcacc tactcggcgg ccattgccgc gtatctggcg aggggctgcg
      721 cgaccagcga ggccgtgcag cgcgcaaaac agtatctcac cggcgccatc cagcacagct
      781 ttccgctcgg agcgggccac ggcccactca accatttctg gcgcacgggc gcataaatct
      841 gccggatacc tggttccctc agggccgcag gcggcgaatc aggaaggcgc cggcgcagcc
      901 ggtgcgggcc tgggaagccc cgcggccgcc aggcgaaatg ccaggcgcaa cagacggttg
      961 ctccagccaa acaagcgcgt gagacagatg gtcgcgcgaa cgctccgccg gctaatcttg
     1021 acgtcggcgc ccgaagtgaa atcgcgccgc cggttgatgt tccgcagcgt cagcaccgcc
     1081 atgccgatcg cccagaggca gaacgcgcgg atacccggct cggatttcgg aatcaacagg
     1141 gtgtagtcaa gcgccacttc cagatgccgg cgggcgacgc cgatcagatg gctcagcgct
     1201 tcctgaaacg ccggattgcc cggtgccgtt tcaagatggc gcaaatcaaa ccccgcacgc
     1261 tcaaacgtcg cctgggggag ccagcatacg tgccgctgcc tgtcttccca gatgtctttg
     1321 aggatattgg tcatctggag tccctggccg aaggataccg cgcggctttc gagctcgttc
     1381 cggcgggccg caactgcttc ggaatgcgca cagaacaggt gcgtcagcat ctcgcccacc
     1441 acgccggcga catggtagca atacgcgtca agatgcgcct catcgcgcag gccgtccgtg
     1501 aactgccctt cctggaactc ctccatgccg tcggacatga tccggataca ccgctgcagc
     1561 gcacgctgct cgtccgcgct gaagctgtgt gtgatgcgca gcacgcactc ggtgttccgc
     1621 accaggtcgc gttccgcatc gagcgtgccg ccgtcaagct tcggggccag gtcctgaccg
     1681 aattccgccc ccgagccgcg ctcatcgagc gcctgcacga accagtcgta gtagcgacgc
     1741 ttctccgcaa tggacaggct ctcggaatct tcaatcgtat cggcgatccg gcacagcagg
     1801 tacgcattgc ccaccacccg gcggagcgcg ggcggcagtt gcgggatcgt cagcgcaaag
     1861 gtccgtgaca cgccctgcag aatgcggtcc tggtaggcaa aggcgtccgc cagtccactc
     1921 atgcttccag catcaaactc tgcaccattc accgcgtgtc gtggtccttg tgacatgcgc
     1981 cgccgcacgt tcgagaattc atcctgattc gccgggccat cgcgcccgca ggggcctttt
     2041 acccccaccg caccccgtga gcacgatccc agagccaccg gcactgcacg gccagccgtt
     2101 ccatccagga ctgcgccgaa taccccgcca ccacgccctt ggattgaagc gcctggtacg
     2161 ataccgtcgc cacatgcttc acgcccaccg cgtaatagag agccacctgc tcatagggcg
     2221 acagattgcg gtgcttttgg taatgcttga gcatgatgtc cgtgcgtttg cccagccgtt
     2281 ccggccgcat cgtcgtgcga acgcacaggt ttgaaatgcc atacgccagg tcttcgagaa
     2341 aacagccgtc tccggcaaat tccaggtcta cgatggcctg caggcgcccg tccttgaaga
     2401 tcagattgcc cccatgccag tcgccatgga tcagcccggt ttcaaactgg ttgcgcgcct
     2461 cataactcag ctcctgagag gcgtcgcgca gaaacagggc aatttgattg cactcgtcca
     2521 tcaccttggc ttcgttgccg ccctctttgg ccatttcgaa aaactgggca aacaaggtgc
     2581 gcggcacttc gctgaaccgc cacatccgaa catcccgttc gggacgggga aaatcgcggc
     2641 agacatcgtg cagctgcccc aaagcatgcg ccgagatcgc caaagtctcg ctggtcacct
     2701 ccatgggagt tccatcgaca aactcctgga gctccagtgc ccagttgtcg ccttccacaa
     2761 tcttcctgcc ggtcttcgcg ggcagaatgc gcgccacggg caaggcattt tcatccaggt
     2821 gctgagaaag ccggtgctgg aaacgaagcg catccagcac gtacgggtcg cgctggtacg
     2881 tcttgatgag aaacttaccc gcccccgtca tcacgatcag tttgcggtgc cggcgctggt
     2941 gggccatagg taccggcacc ggcatgtcga caaagcccac gtcataaaat tcgcgtatga
     3001 cgcgggccag cgcctcctgc ccctgcaggg ccggctgctg tctttctacc tgatcggatg
     3061 tgttcacgga agcgcgatga ccttttcgga ttggacgttt ttcagaagct cctccttctt
     3121 gaaaagcgtc tccttgaatt tcagctggct gtacagttca cggccctgat ccatgtagtg
     3181 cggggatgcg ggatcgttgc tgttgcccca gggcactacg ctgaacgatt tgaccccgtc
     3241 cggccgcatg aacatgagca tcatggccac cgaccccttc cgcgcgacaa acttgccggg
     3301 catctccttg ctttcggcat aacccacgtc gcgcagggtc tccgtcttgt tgctcttgtt
     3361 tggcccgccg ccgaaatcga ccccgccgca ccccacgaag gtatcgcccc ggcccagcag
     3421 atgaatgtcg ccccacatcg gatcaacggt gccgtacatc gccttcatat cggtcacggt
     3481 cttcataaac agatccgcca atttgacctg gtctgcgggc gaaggctcct cgccccgggc
     3541 aatggtttcg acatccaccg cgttttcgca gagaagccgc caggctttca tgatgggcgc
     3601 ccccttgctt tcggggcgga catagccatt ccacgacagc gcggaatcca gcgcggcctg
     3661 taaatcggcg tcggcaagat attcctggcc cgccgcggct acggccgcct taagcgcctt
     3721 ttgccacggt tcggcgagca gatcgtacac attcatcgcg atcgcgatcg catcctcctc
     3781 ggtaaccgag tcatccgcac ccagcaactg cagggcccgc cttccgcgca tgttcatcaa
     3841 atcccacgac acattgaaaa tatagtccgg gtatttatcg ggcgtcatcg gcgaatccgg
     3901 aaacatcaga gtcgggctga tattgcagtt ctgcatgtag cccgaaggcg gatttttgat
     3961 ctgcaccaga tcggcaatgc cggtgatgcc cagccacttt gtggccgacg tgtagccggg
     4021 caccggcgcc gaccaatcat acccttcagg gcggatcggc gtgcggcccg tccgcacata
     4081 ctgaatattg ccctgggtgt ccgcgaacag caggttctgc gccatgaatt cgttcatggc
     4141 cagtgcctca tagaactcgt cgcagctgtt cgccgtcgac atccggtaca cctgctccaa
     4201 aagccccacg gagtccatgt acggggtagc gcccgcatac gcgacgccgt tgtccggccc
     4261 ctccacgcac gggccccatt ccgtgtagac ggcagtacgc tggaacggct cgccatcctt
     4321 gacgtcgaat tcaatcaccc tggtttcggc gtctttccag accccatcga gctcgtactg
     4381 cagcgggttg tccgggttga tcttcagctc atagacatcg gacgtgtctg gccctcctgt
     4441 cgtgcaggcc cagcccagat gggctgtgtg acccaggccc atcatcgggg cgcccacgac
     4501 aaaatgaccg ttgatgtcga cttcttcggc cttcagccgc gcttcgtaaa acacctgtat
     4561 tccgcgccac gtcagatgcg gatccgtcag gagaatggcg cagtgatcgg cggtgcggct
     4621 cggcgcgatc gcccacccat tcgaaccccg gcccggatcc tccttgcggt tcttgagatc
     4681 gccatacacc gtcccgagcg gccattgcag aatcatggcg cgcccgatcg caatgcaatg
     4741 ccagggttca aactcaaacg cgtagtccgg cgtacgttcc gggtggtccg ccacataggc
     4801 attgatcccc gccgcaaacg catcgcaaat caagcggacg cggtccgggc tttccgtgta
     4861 cgcgcgctgc gatacttccg gattccggaa cagccgatgg atgtaatcat cctcaatgtg
     4921 ccctttaccg aagatctccg acatgcgccc gagggccgtg cgggcattct ggaaaatgtc
     4981 ctccagccgg tcctccgcct cggcataccc aaaggcatac atggccgctt caagtgaatc
     5041 cgagtagatg tgcggtatgc cccatttggt gcggtagact gtggcctgcg cggcggccgg
     5101 cgcctccccc ggtgcggagt catccggtcc cgcgacttcc tcgggcggga gggccgtcgc
     5161 cggcaagtgt tccggcgccg cctgggtcac aggttgctgc ggttcccggc aaccagccgc
     5221 aatcacagcc acaagcatca gaaccaggcc cagccgcgaa tacatcgttc tcatcaccgc
     5281 gctcccatcc gttcgtctcg ttatccaccc cgtggaccgg cagccaccag cccaataccc
     5341 cagggcctgt tcaggcgccg cgcgcccggg ccaggcaggc tcgcagatac cggatatcct
     5401 cttccgctgc gacgtgcggg tcgcccgcgc cgctcccgcg gcaactgttc cgggccaccc
     5461 gcccgtaatg cgcgctctcg ggttccccgg cttccccacg gcgcaatcga ttacgcggtc
     5521 atactaccgt ttgcatgacg atacgtcaaa gtcccggagt aagccgggcc tgaatcgcgc
     5581 ccgctgtgtt atgctgtcgg cccatgacac atatacaccg ttttttcgtc gaagcgggcg
     5641 atgtgcaggg cgatgtagcc atcgtggccg gccccgaagc ccatcacgcg ctgcacgtcg
     5701 tgcgcgtgca gcacgggcag ccggtgtgcc tgttcgacgg cctggggaga gaactccagg
     5761 gactcgtgcg agaggcctcc cgccacgatg tgacgattga tattacagat gagcgcaccg
     5821 tccagcctga aatccggacc gtgaccctgc tgcaagcctc acttcggcgc gacaaagccg
     5881 tcgaggagct cattcgccgc tgcacggaag tcggcgtcca ccgttttgtg ttctttcccg
     5941 cggagcgctc cgaacatccg cccagaatca gcgagaaatg gcgccggtgg gctatcgaat
     6001 cgtgtaaaca gtgcggacgc ctctggctgc cggaattttc gctcgcgccc gggctcgagg
     6061 ccgcactcgc gggtacctgg tccgcgctgc ttattgctac tgccgacgag gcgcccgtcc
     6121 cactgcgctc ggccgtccag ggagactgcg tggccctcat tgtcgggccc gaaggcgatt
     6181 tcacccccgc ggaacgcagc ctcgcgctcg cctgccaggc gcaccccatc tcgctcggcg
     6241 ccgctacctt ccgcgcagag gttgccgcta cccttgccgc ggcgctcatc cgctacgaat
     6301 tcggtgcgct cggtccctga cccgccaccg tccgaaaacc ctcgccgcgt tgtggcatcc
     6361 catccacagt gctctctctg gattcgtgga gttcagttgt accggcggcg ccgcttgcgg
     6421 tactgtctac gtaagatcaa aaacagggag agtccggatg gctgaagaga cgatttttgg
     6481 aaagatcctc agcggagaga tcccctcgca agaggtgtac tccgacgaag aattttatgc
     6541 gtttcgcgat gtgaaccccg ccgcaccagt gcacattctg atcattccgc gcaaacccat
     6601 cccgaaagtg acccacctca cggaggccga tgccgccctc atggggcgca tgtttatcgc
     6661 cgccaacaag attgccgagc aggaaggcct cgcggaaaac ggctttcgct atgtcatcaa
     6721 ttgcggtgaa tggggcggcc agacggtctt tcacctccac ctgcacatcc tgggcgggcg
     6781 ggccatgacc tggccccccg ggtgatcgga gacagacgca tgcgcatcga cgcccacacc
     6841 cgcctctgcg ccgtgatcgg gaatccagtc ggacattccc tttcgccgct cctgcacaat
     6901 gcggcgttcc gggcagcggg tctgaattac atctacgcgg cgttccgagt cgaagatgtc
     6961 gccgcctgcc tcgcgggcat gcgggcgatg cacggtttcc gcggcatgag cgtcaccatc
     7021 ccgcacaaac tggctgtcat gtcctgcctg gatgaaatcg atcccatggc ccgccgcgtc
     7081 ggcagcgtca acaccattac caacgagaac ggccgcctga cgggctcgac gaccgatggc
     7141 ccgggcacct tgcgcgcgtt tcaggaggcc ggcatctcgc tcgacggaaa gcgcgtgctc
     7201 ttcatcggag caggcggggc cgtgcgcgcc gtggccttcg ccgtggccga gctcacccgc
     7261 gccgaagcca tcaccgtgat cgggcgcaac gccgaacgcg tccgggccct caccgaggac
     7321 gtggccgcgc aaaccggcag gccaattgag gccgccggct taccggcagg gcttgcaggc
     7381 gccgtggccg aaagcgatgt gctgatccag ggcacgcccg tagggatgca tcccaacccg
     7441 ggccagtcgc ctgtcccccg ggaatggctg cagccccgcc atatctgttt tgacatggtc
     7501 taccggccca acaaaacgca gtttatcctc gacgccgaag ccgccggctg tacgaccctt
     7561 ctcggcattg aaatgctgct caaccaggct gctttgcagt ttgaattgtg gaccggcgcc
     7621 cctgcgccgc tcgaggccat gcgcaacgcc gcccgcgcgg ccttcaacga accgccgtga
     7681 ccgcacggcc cgcgcctgcc gtggcccgct cacgcctgca tgcccgcctg gcgcgcgccg
     7741 tgcaaagagg ggccgccgca acaaaaggat atgccgctat gaaatctcca tggactctgc
     7801 gcttcattcc attcgctttg gccgccctgc tgctcgcggg caactcagcg aatgcggaat
     7861 tcccgccttt gccggagggc gcgttcagcg tcgtcgcctt gcccgacacc caggcctatt
     7921 ccgcgaaggc gccggaagta ttcgccgcag aaatgcagtg gattctcgac aacatcgaaa
     7981 gtgaaagcat cgtctttgtc acccatctcg gcgacatcgt cgacaacaac aacatccccg
     8041 aacaatgggc cgtggccagc cagagcctcg cgatgctgca tggcaaaatc ccgtacggtc
     8101 tcggcgtggg caaccacgac atgaagggca gcaccggcga cagcagtaat ttccaggcgg
     8161 ccttcccggc ctcgcttttc ctcgattttg actggtacgg cggccagtac cgcaacaacg
     8221 ccaacagctg gcagaccttc tccgcggcag gcatggattt tctcatcgtc catctcgaat
     8281 gcaacgcgcc cgacgacgtg ctggcctggg ccgatggcgt catcgccgcg cacccggacc
     8341 gccgcctcat tatcaccacg cacatgtatc tgggcccgcg cgaaaagccg gtcgataaga
     8401 acgatttctt tgacgccccc aagggccgga tgaagtggaa gaaatgccac ggtgacgccg
     8461 ggaacacccc gcagcaaatg tgggacaaat gcttctcccg gcaccccaac ctaatcatgg
     8521 tcctcgcggg cgaccagagc cgcactcaag cgtattatca gaccgcgatc ggcgtgcacg
     8581 gcaacgtcgt ccatgaactc atgtcggatt accgcgacgg ctatttccgc gtgtatcgct
     8641 tcctgccgga cgagaatcgc gtcgaggtct tcacctacag tcccgtcctg aaaaaactct
     8701 gcgatggtac cacgatcgtt ccagatccgg accggcacca gttctcgttt ttctatgccg
     8761 aagaacccgc gctggccgaa gcggccccgg acaagtagaa gtcaagccgt tggctggatg
     8821 aagaccctgg acacggcggc ggcgccgcgg caggcacacc gtgctgaacg cctgtagcgg
     8881 cgctggcgct ccagcctcct ccagtctcct gccaagcacc ctgcgtccgt cctccggcgc
     8941 cctgccagac taccccgtca gctgagccca gacacaaaaa accggccccc gcggttaaat
     9001 gcccgcgggg gccggaagtc gtcatgaccg tttatccggc cgtgagacgc gcattagaac
     9061 tggatgcggg tctcgaggta cagataatcg gcgtcgtcgt cgctggtgcc accgctgaag
     9121 ccaaggccgt tgaacagaac gtacgtgcca tcggtagcgc catcgtcaac aaacaggtgc
     9181 gcccacccgg cttcgaacac caggtcttcg ctgtagtggt aggtcgcttt cagctccgtc
     9241 tcgataccca ggtcgcggtc gttctgcatc gtccagaagc tgaagaacgg ggcgatcggg
     9301 atccgccagc cgcccagcgt gatgtgcgcc ggcgcatcga attcctcgag cgcacggaag
     9361 tacttcacga tcagggccag ctcgatgctc tcggtcggca tggcctgcac accaccgcgc
     9421 acggtccaca tgttggacag cgtggtgtta tccaggaact ggctgtattc ccagttcgag
     9481 aacaggcggt tgaagctcac actggctcgc ggagagctga aggggctgat ccattcccac
     9541 cagctgatgt cgcgcttgtc ttggccttcg aagtacgcgc cgccgaccca gacacgcggc
     9601 tgccacgcca tgtcaaacgt gtaaccgact tcgaggttgc ctgcccacgc actgaaatca
     9661 gcgctgtcat caccgtaaat gtacggacgg aacagataac cgacctggtc ggcttcgcca
     9721 aactgatagg cgaactcggc ttcgaaatca aacgcgccaa tggtgccagc tccgcggagg
     9781 ccaacggtgt gcagattggt cggatcgtag tcatcgatcc cgaagatgtc ttcccaccac
     9841 tcaccaaacc aggtgaggtc ggtgtcgctc agacggcggg catcgcgcag ccacagccag
     9901 taggcatcga tcacgatgtc ttccaggccc aggtagctgg catacacgcc atacagccag
     9961 atatcttcgt cttcttcaaa cgcaccgcct tcaaacagct gcgccatgaa cgcatccacc
    10021 gagaacatgt cggtcgcgta ggtcaggcga actgcgtcaa aggaaagacc ggtgaagccg
    10081 gaagaagtgt cattcacacc gaccagccac tcgctgccga agctgagctc ctggcggcca
    10141 acacgaagcc gaagcggcag accgaacatc tcgttcgcct cgatgtaggc ctggtagatc
    10201 tcaacgtcgt caccggtcca cgcgcggctg tccgcaccgg tgatgtagtc ggaacgaaaa
    10261 tcttcgcccc agatgtcgta cgaatcgagc tcgatgaacg cagcaacttc attcgagaag
    10321 tccgctttca cgttcaggcg agtgcgctgt tcgacataac cgagcgagtt ttctttgtcg
    10381 tcccaaccaa tcgcgccgcg aacaccgtcc caattgtaat tcgagccggt gccgatcgag
    10441 cggccgctga gaagcccagc cggccaatgc aggttcgaac ggttgatgtt cgtctgagtg
    10501 ttcgtatagt agttgccgcg aatgcggacc tgtccaccaa caatcacatt ctgcagttcg
    10561 gcgagcgccg gcgttgccgc aagcactacc accgctgcaa gtacaagaaa catgctttta
    10621 cgcatcgtgc gtgttctccc taagatgtat acataccagc cctcgcgcag gtttcataac
    10681 cctgtgcgcg ggttctgacg tttctccctg aatctatcga acacatcgat gcttgacgac
    10741 cccggagcag tctcttagac ttctgcatcc ctcctttcgg ctccagaatg aggtcatcaa
    10801 tgatggtgtt gtcatgccgc tcaaggcatt ttgtcaagtt aggggacggc cacacgtgcc
    10861 tggcacgaaa acctctgcaa ccgttactcc ccagtaatcg ttgacaagtt tagcacactt
    10921 cccactcaac gtcaactact tctattgaca acttgggccg ggattttatt cattatgccc
    10981 ggcacatggc agattttact tgccagaggg cgcacatttc acgcgaaaat cgagtaaaaa
    11041 gagttttacg agaataaaat acggtgtata caatatatcc gatctcgccg gtgtcttgcc
    11101 tcacccaacg ctccccgccg gagcacgcgg cagggcacag actgctggct ccgccacgtt
    11161 cggaatcacc cttgactcag cgcttactct gcccctgcgc tggccttccg cgccaactcg
    11221 cgcaggtagt cccacgaata gatgccggtc gtgtggccgt cggaccactc aaacgagatg
    11281 gcgtaattgc cgaggtaatt gaccctggaa aaccggattt tatccggaat gctggcggga
    11341 tcgagcagtt tttcgcccgt gtactcgtcc acgcagaccg cacacggaca cgccgcgcgc
    11401 aacatccggt tgggcacttc cgccacaaat ccatccggcc acgtgaattc caccatgtac
    11461 tcccgttcga ccacatccgg cagctcttcc ttgcccgcgc ggctcttgcc aaccgcgcga
    11521 tggacgttct ccgccagctg cgccatgacc tccaggtccg cccccgcgtc cggcgacgac
    11581 agatccgaca cgccgggcat gatcggcagc tcggccaatg tctgcaggcc gaaccgttcc
    11641 tgaagcatcc cctggttgcg tccaaacggg taatgcacct tgccgcatcc gtcgcaggtg
    11701 aacgagcaca tgttctcgac aacgccaagc acgggcacat tcaccttctc aaacatgaga
    11761 atgcctttgg cgacgtcgac cagcgacagg gcctgcggcg tgcttacgat caccgcgcca
    11821 tcgagcgccg cctgctgtac aatcgtcaat tgcacgtcgc ccgttcccgg cggcatgtca
    11881 atgatcaggt aatcgagctt gccccagtcc gtctgcagca gaatctgggc tgtgtagttg
    11941 gataccatcg gcccgcgcat caccgcgggt gattcgccca gaacaaaacc cagcgacatc
    12001 accttcagcc catggatatt cagcggcacg atcttgttgt cgtggccaaa cacctgcggc
    12061 cggtgcttgt tgaacagcgt cggaaacgac ggcccgtaga tgtcggtatc cagcaggcca
    12121 acggcgagcc cttcccgggc catggccatg gccaggtgcg aggcgacagt cgatttcccc
    12181 accccgccct tgcatgcgga aaccgcgatc accgtttcga tgtttttcag gttttcagtc
    12241 atgcccggcg caccctgccc ctgaagccgg cgcggcgcct gcgcgctcag cgtcacatcc
    12301 acgctggtaa cgcccggcag ctcgatcacg gcctttttgc acgactcgag aaaatggctc
    12361 ttcatgggac aggccggtgt cgtcaattcg agagtaaaac tgacgtgccc tccctcgatt
    12421 ttcaggtttt tgatgaaccc gcaagacaca atgtcgcgcc ccaggtccgg atccatgata
    12481 tggcgcatcg aatcaagtac ttcgtgttcg ctcggcatag aaatctcctc ggcgcgatac
    12541 cgcgcgtggc aaaaacagac gtagacggac atcccgcgag cccgggatcg gcggcgacgt
    12601 tctctggctc cttcaagcgc cgcagacagc cgcggcgccg gggtctgcgg ctcgagcctg
    12661 cccggatcgg ccaggtacgg ctggttcact cgacacgctt cagccaaagc ggcctggcgc
    12721 aacacggccc tgaattcgtg taagaaccgg gccaggccgg aatcataccc gcactcgagg
    12781 gagtgagcca aatctgcggc cggcagccat ttctgtagca acgtcattca gccggcatga
    12841 aattccagag cggcgctcaa accatctccg gagcttcggt tgaatccgcg gtgacggcgg
    12901 gttcgagatg aaattcccgt atgacttctt cgatggtcat cgcgaagatg ccccctccgg
    12961 gttcgtacaa gcgttcgagc aggcgcttca tggtctcgtc gtcacgcagg gccgagggat
    13021 cgcgcccgtg ttcgcgcacc gcgcgcagca cggccccaac ggtgatcttg tcgatggatc
    13081 gggcgggctg gtaacggaca ggctccgaat tgacctgccg gagcaggcct gcctcttcga
    13141 gttcgtcgag cccgtcgttc agcagccgca cggggacatt ccattcggct gcacattcgg
    13201 ccgcagacag cggaggcagg ccctggtcga accggcggct gacctcgata accgagcgca
    13261 ggcagagggc ctctttgtag gcatggctcg cgtgctcggc gaggcgttcg agggcaaagg
    13321 tcttctcgta ctggtaggca aacgtgatct gggcgccaaa caggatgatc agccagcagg
    13381 tgtagatcca caccagcagc agcggaatca gcgcaaaact gccgtaaaag aagttggccc
    13441 gcgcgacgcc ccattgaaaa tgcagaaagg ccttggcggt gaagatccag atcgtgcccg
    13501 cgacgatgcc gcccagcagc gcgtaccggg ccggtacccg ggtattggga acgacacggt
    13561 agaccgtggc cagggccagc cagagcacca ggtaccgcgc gacgctgaga agcaggcgca
    13621 gggagcccca ggccaggttt tccgtttcca gcgccgtggt caggcccacc acaaaggcca
    13681 ccacaaacgg aagaaacagc acgatgagca tgtaatccgt gaaaacgcga aaccacgagc
    13741 ggttgcgttt ggtgccccag atcgcgttaa acgccgattc aattccgttc atcaggccga
    13801 ataccgagaa aaagacgaac acgatcccca gaaacgtgcc catggccgtg cttttcccgc
    13861 tttcttcgac catctcgagg atgactttga gcgggctttc cgcagccagc tggtcttgtc
    13921 catcctccgg cggcggcacg ccttccagca gccagccgag ggctgactcc aggagttcgc
    13981 ggttgcgccg ggcgcgctcg tcgggatcgc tggaaacagc cctgggcgtt cgcatggttt
    14041 cggcgtgttc ccgcggctcc tgcgggggcg aaggtgggat tgcatcccgt accgctccca
    14101 tgcgttcggc gtagcggtgg atgctgtttt cgagcacgtc ggtgaggctc tgcgccagat
    14161 cgaaggtctg catgatgaaa aaggcgaacg ccagaaacgg cacgatcgcg aggatcgtgg
    14221 caaacgcaag cgacgaagtc cgcagccaga ggacgtcgct catcaacccc cgaaacagca
    14281 ggatggccac gcggatctgt ttgatgagga atccgctggg tacctgttcg ccgggtttgc
    14341 caatacgcca gacatcatag gttacaaacc ggcggccgcg ctgcagggct tcaacaaatc
    14401 ttctgaatag ctcagccatg cctccatgct acagcctggc atcttcccgc gcaaccaaca
    14461 gcttacggcc ctatgatgac cttcgtgccc acgggacacc actgaaagac agcctcagcg
    14521 tccgccggcc gcatccggat gcatccgcga ctcatgttcg cgccgatgga atgcggctca
    14581 ttggtgggat gaatgccata cggagtaacc cggccgtctc tctcgagccc cagccaatga
    14641 cggccaaggg gattctcagg atcgcccgct tttacgattc ggccccgatc cgaccagtcg
    14701 ggatcggtca ctttgttggc gatgagaaat tccccctctg gcgttgcatt gttgcgcccc
    14761 agaccgaccg gaaaccgggc cagcagggcg tcgccactct ggatgctcag gatatggtcg
    14821 cgtttggcga catgaatcac taccgaatcc gggggatact cggccggaga tgctgcccgg
    14881 accgcgaggg cctccgaacc cgccggactg gcatcagggc cgcgtgccgc cgtgtctgcg
    14941 ggggacaact caggcctccc gtccgccgaa acggcctcct cgacgttcgc ggcgcctggg
    15001 gaagaaaatg gagggatatc cccggacgag tgtgaaccgg tggccgtcct ctcccctggc
    15061 cctctccccg gcgctgcggc tttctcaccg gacacgcccg tggccccgga aagcgactgg
    15121 aggt
//
LOCUS       DAOPBQ010000020        14999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4028_length_14999_cov_4.511710, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000020 DAOPBQ010000000
VERSION     DAOPBQ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14999
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4028_length_14999_cov_4.511710"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PKW60_01500"
     CDS             <1..364
                     /locus_tag="PKW60_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006036662.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPJ98135.1"
                     /translation="NAIAAHHNEQEKSSVIAVLVQAADALSAARPGARRETIESYVKR
                     LEQLEALANGFEGVEKSYALQAGREVRLAVEPEKVDDAQAAQLARDVARKVEQEMTYP
                     GQIRITVIRETRATETAN"
     gene            395..1186
                     /locus_tag="PKW60_01505"
     CDS             395..1186
                     /locus_tag="PKW60_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPJ98136.1"
                     /translation="MNILFIGDIVGRPGRDSVGYWLPRLHDEFAIDLVVANGENAAGG
                     LGITAAILRELQGLGIQVITLGNHTWRKREFVKAISAFDNVVRPANYPDGVPGLGGVL
                     YTLPDGRTVGVVNLLGRVFMEPVSCPFATAARVVAELRQATPLVVVDMHAEATSEKVA
                     MGWHLDGSCTAVIGTHTHVQTADERVLPQGTAYITDVGMTGPLDSVIGVERDLVVSRF
                     LTGMPVEFKVAKGRPALGAVVIEAEEHTGKARSITRLLREDPEYG"
     gene            1214..4801
                     /gene="smc"
                     /locus_tag="PKW60_01510"
     CDS             1214..4801
                     /gene="smc"
                     /locus_tag="PKW60_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPJ98137.1"
                     /translation="MYFKQLELSGFKSFADRTVVRFEPGVTAIVGPNGCGKSNILDAL
                     KWCLGEQRARELRGSLMQDVIFNGSENRNPLGMAEVTVLFDNADSKLPVDFAEVAVTR
                     RLYRSGESEYLINNAPCRLKDIQELFMDTGIGTSAYSMVGQGKMDLILSSKPDDRRFL
                     FEEAAGIIKYKNRKRAALRKLESAENNLLRLSDIIAEVQRQMRSLKRQVNAAIRYREL
                     TEQLRDVEIRAAWLKHTALTSEITEFEQRFAQARDEYEAASTRITELDARFEELGLAK
                     LEQDRVLLSRRESVHEVDSEMEKIEREIALTRQTITFSEEQERRAEAEGGEFEQRAEA
                     IAAHIELTRAEREQAQSELSECSAAVDAQAAEHAALTETVSRADACLEEKRAQALEKM
                     NTRARAATELEALGINIANIEAQLEAIYENQGQIDQRREELLRELEQQRTHESDAQRR
                     LTESGAARAQALQLQTGKAQSLRTLDQEWQELRETKSSLDARLTSLRELRDNYEGFAA
                     GVRAVMRAKSDGHAGMQNIIGPVGDLLSTEKEYERAIEAALGGNINNVVAEDAGAAKG
                     AIDYLKSNQAGRVTFLPLDILRSSGRESDSLMGRPGVIGEAIAFVQFDEHIRPAVDYL
                     LRNTVVVEALDDAIRIAREKSSYPKLVTLDGEVMQPSGAVTGGRTRHERGGLLGRSAE
                     ITDIEKQLLDLEQRFSEIADKGQVLNTELQELGAQIREHEAAEDALRSELNGLGVLIA
                     RQSTELDSLMERSRNLNAQRDELCGRRDTLEGQRREAAARADTMASDDEVMQHEIAEA
                     QEAASHARQALSMCASELADLRVRQAGVAQRIQEIERDVEREELRRQEALNEAQRRRE
                     AGVARQEERRRLEEAIRLHVERVRALSETKEQARGKVIEAENQRQTLLDESEGVEKQL
                     REKREQARDAQSRLHEAELALRQREDRVGYFQERILEAYNVALASLREEEVGTDEYSS
                     EERDQIVTDVRSRLQRLGEVNLMAIDEYDALEKRESFLTAQHKDLLSARETLLGVIAR
                     SDKKILAMFLETFNLVADHFRNYFRTMFNGGQARIYLLNEDDPLESGIEIEARPPGKK
                     LQSISLLSGGEQAMTALALLFAIFKAKPSPFCILDEVDAPLDDANIGRFLSMVEEFTD
                     ESQFVVITHNKQTMARGNALYGVTMQERGVSQIVSVRFTDDKESTGSAA"
     gene            complement(4881..5930)
                     /locus_tag="PKW60_01515"
     CDS             complement(4881..5930)
                     /locus_tag="PKW60_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98138.1"
                     /translation="MAAKPLRVGVVGTGFGRYHMDGFSQVPGVEIRAICDLNKAEAQQ
                     FADQYGARHVFTSAKRMFAMDELDAVSIAVPNALHAPMTIDALSKGKHVLIEKPMTIS
                     PADAQKMADAAKKAGRLLMVEQALRFRPEAQLVKAYAERGEFGDMYYARSTWYRRKGW
                     PHLHMPPDGTLGRGVWFLQKRLAGFGALGDIGVHLLDLAWYLMGTPKPVSVSGQLFRE
                     VATPELKAKGLPAEVEEMAAGLIKFQGGKAIMVEVSWDTYTKPAMECFLMGNRGGASV
                     FPAQVFRGTDIDETVDIETHYNGLPIETAFSHFIDCIRDRRKKCIAPADQNVTLIRML
                     DALARSAKSGKEVRL"
     gene            complement(6015..8573)
                     /gene="glgP"
                     /locus_tag="PKW60_01520"
     CDS             complement(6015..8573)
                     /gene="glgP"
                     /locus_tag="PKW60_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPJ98139.1"
                     /translation="MPRIKEFTVVAKLPPVLEPLRSLAYNMWWSWDPEAIDLFFRIDR
                     DRWITAKQNPVRLLGEVSQERLEELTHDDGYMAHLERVHSRFQAYLRGNSWHEKNPQA
                     PKEFQAVYMSAEFGLHESLALYSGGLGVLAGDHLKAASDMGLPLVGVGLMYREGYLQQ
                     YLNADGWQQERYPLNDFYNMPVTLLRDETGKQVTIEVEYPERPVKARVWRIDVGRVPL
                     YLLDCDFDANEPDDREITARLYGGSTDMRIRQEILLGMGGVTMLRKLDIHPTVWHMNE
                     GHSAFMTLQRIKNLVKGEELGLEHAIESVKAASVFTTHTPVPAGNDMFLPEMMEHYFH
                     KYAMDVGMNMDRFLGLGRQDPSDHREPFCMTVLALKLSAFANGVSELHGATARAMWAR
                     TWAGVPEDEVPITSITNGIHQQFWVSRDLAGLFDRYLGPGWISNPSDPAVWKNIDQVP
                     DAELWRTHERRRERLVNFARRRLAAQLKSRGAPNAEVEAASEALDPEVLTIAFARRFA
                     TYKRAKLLLSDPERFIRILTHPERPVQIIFAGKAHPADNQGKDLIRALIHFMRENDVR
                     NHFVFLEDYDMNVARYMVQGVDCWLNTPRRPLEASGTSGMKSAANGGLNISIPDGWWC
                     EAEGLGENGWSIGRGEMYEDPDEQDVVESEMLYELLEQEIVPMFYERSSDDLPRRWVM
                     RMKNAMRTINPIFNTHRMVQEYTDRFYVPATQRRVFMQENSRARSKALAEWKMKVRAC
                     WSQVRFTDFESGPLDDLPYGSNLEVRAGLHLDQLTSSDVIVEVYYGTVDSYGRIHDGR
                     HTPMLLDEKASDGVYRFKGSIYCDKTGQQGFTVRVMPSHPDLADKHETALITWA"
     gene            complement(8689..9981)
                     /locus_tag="PKW60_01525"
     CDS             complement(8689..9981)
                     /locus_tag="PKW60_01525"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPJ98140.1"
                     /translation="MMGIIWPILLGLGALAEAVPASQGPLPAGLDTGWAAQADQLRQT
                     VREILTFPAERVALDAQTHRSIDGEGYRIECVTYQSEADSRVTAHLYLPAPADAPVPG
                     IVLACGHGGSKSSIYYQYAGQLYAKLGFACFIPDTIGEEERHKDGKMGTRAHDMYEFG
                     KDSAARRAFTREKLGRMVLGKIVWDLVRGLDYLETRPDIDPARLGVMGNSMGGTTSSL
                     VAAIDTRVRAAVISGWSMTAQGAIQGKDCSKMPFEALAHAMSFDELNALLASHAATLF
                     INGGQDSIIDVAEGGLAVVRRTRATVAGARQILADAGIPGIIEAQFVAGACHRPYILT
                     RPAAAWFQEYLQAPEDRRPLPAATIKLGDWVDAHGLKLEPLYDTEPRQRGTDALDIGA
                     QYRVPAELACFPGSASPDPAYTWEGWKERRITESEARD"
     gene            complement(10266..11336)
                     /locus_tag="PKW60_01530"
     CDS             complement(10266..11336)
                     /locus_tag="PKW60_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPJ98141.1"
                     /translation="MFDGLGMHLGNLSRLSTAKTRSISAENFDGAKGGGGMATDGTGA
                     QCARDLGQGWKISPSVCIEPGQTFELASIDGPGAIQQIWMTPTGNWRFSILRVYWDGE
                     DLPSIETPVGDFFCMGWGEYAHVNSLPVCVNPGSAFNCYWEMPFRKHCCMTFDNIGDE
                     PMVLFYQINYTLTSVPDDCAYLHAQFRRTNPLPYKETYTIADGIRGHGHYVGTYLAWQ
                     VNNTGWWGEGEIKFFLDGDGEFPTICGTGTEDYFCGSYNFDRNGHYAEFSTAYAGLPQ
                     VIRPDGAYRSQQRFGLYRWHIMDPIRFEQDLRVTIQALGWRSGGRYLPLQDDIASVAW
                     WYQAEPHAPFPSLPGRDQLEVI"
     gene            complement(11463..12887)
                     /locus_tag="PKW60_01535"
     CDS             complement(11463..12887)
                     /locus_tag="PKW60_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPJ98142.1"
                     /translation="MSRRHFLAGSAAGVALAHAAHAGTESPVSKPNVVFVFADQWRAQ
                     ATPFNKDPNVQAPYLARLAAESVNFTRAVSGCPVCTPYRASLMTGQTPLTNGVFMNDV
                     PLPSEAVTIGKVFKNAGYDTAYIGKWHLGGRGRSSYIPPERRQGWDYWKVLECTHDYN
                     HSAYYADADEKLLWDGYDAIAQTRDAQQYIRAHARAEKPFVLFLSWGPPHEPYQTAPE
                     PYRQRFRPEDIVLRPNVPPEAQEQAQTDLAGYYAHIAALDDCLAALDQTLAEAGIAED
                     TVFVFASDHGDMLGCQGVWKKQWPFEESIRVPFLLRYPAAFGRQGRQLDAPIDAPDIM
                     PTLLGLCGIPVPGTVEGLDYSGYIGGGPNPGDGTAVIMCPQPFGQVSRKHGGREYRGI
                     VDGQYTYVRDLNGPWLLYDNASDPCQLQNRCNDPGMAEVQAKLDAQLSVRLAQRNDEF
                     LPGRAYIERFGYTVDDTETVPYQP"
     gene            complement(13018..13770)
                     /locus_tag="PKW60_01540"
     CDS             complement(13018..13770)
                     /locus_tag="PKW60_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPJ98143.1"
                     /translation="MICAGDGLTPKQREIREREEHILETARQMFFEAGYYNLTMGKIA
                     KRINCAKGTVYLHFPSKEDIIVALAERAHEERYAMLRRAMGFRGCSRERLLAFCLAGE
                     YYARLNPGDMQLMKTITRSFREKISPERGARFEKLEQRIINYLMRMVHQAVTDGDLVL
                     RPGTTVGRIVWALWALADGGYGIVLSGVSLEALGIEDPWGMVASFAGKMADSLGWKPL
                     SSEWDYDASVKRIQREVFAGEARQLGEFPADA"
     gene            complement(13998..>14999)
                     /locus_tag="PKW60_01545"
     CDS             complement(13998..>14999)
                     /locus_tag="PKW60_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPJ98144.1"
                     /translation="KEGQFKGKSDSLFTAANAANITFRGYGATLRMRKADYQSDAYEK
                     AEWRMGLAFRGCRNIRVEGLRLEGSGGDGIYLGATQELAWCEDVVIRDVICHDHHRQG
                     ISVIGAVNLLIENTVMTDTSGTAPAAGIDFEPNHPREKLVNCLVRNCVFSGNQGAGIL
                     LYLKNLDAGSSPVSLTFEDCFVGAGNSSGIVIGAVNDQGPGGQVEFRNCTVENPHEHG
                     LRIYDKAASSVEIRFTNCHWSGSWSQPGKQADVPRSPLEIELRRQQITREQGGVAFDA
                     CYVYDTVDRPVLAIREHETEFGARNVSGTLFVVSPHAPRMDPGPNPKDVAVELVHLP"
BASE COUNT         2835 a         4527 c         4789 g         2848 t
ORIGIN      
        1 gaacgcgatt gccgcacacc acaatgaaca ggaaaaatcc tccgtgatcg cggtgctggt
       61 gcaggctgct gacgcgctgt cggccgcacg ccccggcgca cgccgtgaga ctattgagtc
      121 gtacgtcaaa cgcctcgagc agctcgaagc cctggcgaac gggttcgagg gagtcgaaaa
      181 atcctacgca cttcaggccg gacgcgaggt gcggctggcc gtggaaccgg aaaaggtcga
      241 cgacgcgcag gcggctcagc ttgcccggga cgtcgcccgc aaagtcgagc aggagatgac
      301 ctaccccggc cagatccgga ttaccgtcat ccgcgagacg cgggcgaccg aaacggccaa
      361 ctagggccgc agcaattacg agcgaaccgc aggcatgaac attttgttca tcggggacat
      421 tgtcggacgc ccgggccggg acagcgtcgg gtattggctg ccgcgtttgc acgacgagtt
      481 tgcgattgac ttggtggtgg caaacggtga aaatgcggcg ggcgggctgg ggattacggc
      541 tgccattctc cgcgagctcc aggggctggg gatccaggtg attaccctgg gaaaccacac
      601 ctggcgcaag cgcgaattcg tcaaggcgat ctcggccttc gacaacgtgg tccgcccggc
      661 gaactatccc gacggggttc cggggctcgg cggcgttctg tatacgctgc cggacggccg
      721 gacggtaggc gttgtgaacc tgcttgggcg ggtgtttatg gagccggtgt cctgcccgtt
      781 tgcgaccgct gcgcgggtgg tggctgagct tcgccaggcc acgcccctgg tcgtggtcga
      841 tatgcatgcc gaggccacat ctgaaaaagt ggcgatgggg tggcatcttg atggatcatg
      901 cacggcggtc attggcacgc acacgcatgt ccagaccgcg gatgaacggg tgctgccgca
      961 ggggacggcc tacatcacgg atgtaggcat gaccgggccg ctcgattcgg tcatcggcgt
     1021 agagcgcgac ctcgtggtgt cgcggtttct gaccgggatg ccggtggaat tcaaggtagc
     1081 caaaggacgg ccagccctgg gggccgtagt catcgaggcg gaggaacaca cggggaaagc
     1141 ccggtcgatc acgcgcctct tgcgcgagga tccggaatat gggtaaccga aaccacacgg
     1201 atttccgttt gttatgtatt tcaagcaatt agagttgtcg ggtttcaagt catttgccga
     1261 tcggacggtg gtacggtttg agccgggggt gacagcgatt gtcgggccca acgggtgcgg
     1321 caaaagcaac atcctcgatg cattgaaatg gtgcctgggc gagcagcgcg cgcgggaact
     1381 gcgcggttcg cttatgcagg acgtcatttt caacgggagt gaaaaccgca acccgctcgg
     1441 catggccgaa gtgaccgtgc tgtttgataa cgcggattcc aagcttccgg tggattttgc
     1501 ggaagtggcg gtgacgcgcc ggctctaccg ttccggcgag agcgaatacc tgatcaataa
     1561 cgcgccctgc cgtttaaagg atatccagga gctgttcatg gataccggca tcgggacgag
     1621 cgcgtattcg atggtcggcc aggggaaaat ggatctcatc ctgagctcca aacccgacga
     1681 ccggcgcttt ctgtttgaag aggcggcggg gatcatcaag tacaagaacc gcaagcgggc
     1741 ggcgctgcgc aagctcgaat ccgccgaaaa caacctgctg cggctcagcg acatcatcgc
     1801 cgaagtgcag cgccaaatgc gctccttgaa acggcaggtc aatgcggcca tccgttaccg
     1861 ggaactgaca gaacagctgc gcgacgtgga aattcgggcc gcctggctga aacacacggc
     1921 gttgacgagt gaaattaccg agtttgagca gcggtttgct caggcccggg atgaatacga
     1981 ggccgcgtcg acccgcatta cggagctgga tgcgcgtttc gaggagctgg gcctggcgaa
     2041 gctggaacaa gaccgcgtgt tgctttccag gcgcgagagc gtgcatgagg tcgactccga
     2101 aatggagaag atcgaacgcg agatcgccct cacccgccag accatcacct tttccgaaga
     2161 gcaggaacgg cgtgcagagg cggaaggcgg cgaatttgaa cagcgcgccg aggccattgc
     2221 ggcgcacatc gagcttacgc gggcagaacg tgaacaggcc cagagcgaac tctccgaatg
     2281 ctcggccgcc gtcgatgcgc aggccgcgga acacgccgca ttgacggaaa ccgtgtcgcg
     2341 tgctgatgcg tgtctggaag aaaaacgcgc tcaggccctg gaaaagatga acacccgggc
     2401 ccgtgccgcc accgaactcg aagcgttggg catcaatatc gccaacatcg aagcccagct
     2461 cgaggccatc tatgaaaatc aggggcagat cgaccagcgg cgcgaagaac tccttcggga
     2521 actcgagcag cagcgcacgc acgaatccga cgcccagaga cggctcaccg agagcggcgc
     2581 ggcccgcgcc caggcgctcc agctccagac cggcaaggcg cagtcgctcc gcacgctcga
     2641 tcaggagtgg caggaactcc gcgaaaccaa gagcagcctc gatgcgcggc tgacctccct
     2701 gcgcgagctg cgggacaatt acgaggggtt cgcggcgggc gtccgcgcgg tgatgcgggc
     2761 caagtccgat ggacacgccg gcatgcagaa catcatcggc cccgtcgggg acctcctgtc
     2821 tacggaaaaa gagtacgagc gcgcgattga ggcggccctg ggcggcaaca tcaacaacgt
     2881 ggtggccgaa gatgcgggcg cggccaaagg cgccatcgat tatctgaaga gcaatcaggc
     2941 cgggcgcgtg acgttcctgc cgcttgatat tctgcggtct tccggacgcg aatcggattc
     3001 tctgatgggc cgcccgggcg tcatcgggga agccattgcg ttcgtccagt ttgatgaaca
     3061 catccggccg gctgtcgact acctgttgcg aaacaccgtc gtggtcgaag cgcttgatga
     3121 cgccatccgt atcgcgcggg agaaatcatc ctacccgaag cttgtcaccc tcgacggaga
     3181 agtcatgcag ccgtcgggcg cggtgacggg gggccgcacg cggcacgagc ggggcggcct
     3241 gctcggacgc agcgcggaaa tcaccgacat tgagaagcag ttgcttgatc tcgagcagcg
     3301 gttttctgag attgccgaca aagggcaggt gctgaacacc gagctccagg aactcggcgc
     3361 gcagatccgg gagcacgagg cggctgaaga tgcgcttcgg agcgaattga acggacttgg
     3421 cgtgctgatt gcccggcaga gcaccgagct ggacagcctg atggagcggt cccgtaacct
     3481 gaacgcgcaa cgcgacgagc tgtgcggccg ccgggacacg ctcgaaggcc agcgcagaga
     3541 ggccgctgcc cgcgccgata cgatggccag cgatgatgag gtcatgcagc acgaaatcgc
     3601 ggaggcccag gaagctgcgt cacatgcgcg ccaggcgttg tcgatgtgcg ccagtgaact
     3661 ggccgatctg cgtgtgcgcc aggccggcgt agctcagcgc atccaggaga tcgagcgcga
     3721 cgtggagcgg gaggagcttc gccggcagga agccctgaac gaagcacagc gccgccgtga
     3781 ggcgggcgtg gcccgccagg aggagcgcag gcgtctcgaa gaagccatcc gcctgcatgt
     3841 ggaacgggtc cgcgccctgt cggagaccaa agaacaagcc cgcggcaagg tcattgaagc
     3901 ggagaatcag cggcagacgc ttctcgacga atccgagggc gtggaaaaac agctccgcga
     3961 aaagcgcgag caagcccgcg atgcccagtc tcgcctgcac gaagccgaac tggctctgcg
     4021 ccagcgcgaa gaccgggtcg gctacttcca ggagcgcatc ctcgaggcct acaacgtggc
     4081 cctggcctcc ctgcgggagg aggaagtcgg cactgacgaa tattcctccg aagaacgcga
     4141 tcagatcgtg accgatgtgc ggtcgcgact gcagcgcctg ggcgaagtca atttgatggc
     4201 cattgacgaa tacgatgcgc tcgaaaagcg cgagtccttc ctcaccgccc agcataagga
     4261 tctcctgagc gcccgcgaga ccctgctcgg cgtgattgcc cgaagcgata aaaagatcct
     4321 ggccatgttc ctggagacct tcaatctcgt cgcggaccat ttccgcaact atttccgcac
     4381 gatgttcaat ggcgggcagg cccggatcta cctcctcaat gaagacgacc cgctggagag
     4441 cgggatcgag atcgaggcgc ggcctccagg gaaaaagctg cagagcatct cgctcttgtc
     4501 gggcggggaa caggccatga ccgcgctcgc cctgcttttc gccattttca aggccaaacc
     4561 cagccccttc tgcatcctcg acgaggtcga cgcgccgctc gacgacgcca acatcggccg
     4621 gttcctgtcc atggtcgaag aattcacgga cgagagccag ttcgtggtca ttacgcacaa
     4681 caaacagacc atggcgcggg gaaatgcgct ttacggcgtg accatgcagg aacgcggcgt
     4741 gtcccagatc gtctccgtgc ggttcacaga cgacaaggag agcaccggct cggcggcgtg
     4801 acgcagcgga ggcccatagc gcgtccgccc cgtcctggaa gctcagcgcc cgggcacagg
     4861 ctgcacccgc cgcatgcctc ctacaaccgc acttccttgc cggatttggc ggagcgcgcc
     4921 agagcgtcga gcatacggat cagggtcacg ttctgatcgg cgggggcaat gcacttcttg
     4981 cggcgatcgc ggatgcagtc gatgaaatgg ctgaacgcgg tctcgatggg aaggccgttg
     5041 tagtgggtct cgatatccac ggtctcgtcg atgtcggtgc cacggaacac ctgggcgggg
     5101 aagacgcttg cgccgccccg gttgcccatg aggaagcatt ccatggccgg tttggtgtag
     5161 gtgtcccacg atacctcgac catgatggcc ttgccgccct gaaatttgat gaggcccgcg
     5221 gccatttcct cgacctcggc gggcaggccc ttggccttca gttcgggcgt ggcgacctcg
     5281 cggaacagct gccctgacac ggacacgggt ttcggagtgc ccatgaggta ccaggcgaga
     5341 tcgagcagat gtacgccgat gtcccccagc gcgccaaacc cggccagccg cttctgcagg
     5401 aaccagacgc cccgccccag cgtgccgtcg gggggcatgt gcagatgggg ccagcctttt
     5461 ctgcggtacc acgtcgaccg ggcgtaatac atgtcgccga actcgccgcg ttcggcgtag
     5521 gctttcacga gctgggcttc ggggcggaac cgcaaagcct gttccaccat gagcagtctg
     5581 cccgccttct tcgcggcatc agccattttt tgcgcatcgg ccggggagat ggtcatcggc
     5641 ttttcgatga ggacatgctt gcctttgctc aaggcgtcga tggtcatcgg ggcatggagc
     5701 gcgttcggca cggcaatgga aaccgcatcc aattcgtcca tggcgaacat gcgtttggcg
     5761 cttgtgaaca catgcctggc gccgtactgg tcggcgaact gctgtgcctc ggccttgttc
     5821 aggtcgcaga tggcgcggat ttcgaccccc ggcacctggc tgaacccgtc catgtgatac
     5881 cgtccgaacc ccgtgcccac aacgccaacc cgcaagggct tcgctgccat gactgccata
     5941 ctcctctttt ttggattccg tgcgccggga attcccgtgc cacgcccccg gaaccgtcct
     6001 gcgttcgggc ccgatcaggc ccaggtaatc aatgccgttt catgtttgtc cgccagatca
     6061 ggatgactgg gcatcacgcg taccgtgaaa ccctgctggc cggtcttgtc gcaatagatg
     6121 gagcctttga accggtacac gccgtcgctg gccttttcat ccagcagcat gggggtgtgg
     6181 cggccgtcgt ggatccggcc gtagctgtcc acggtgccgt aatacacttc gacgatgacg
     6241 tcgctgctgg tcagctggtc gagatgcagt ccggcgcgga cttcgagatt cgagccgtac
     6301 gggagatcgt cgagcgggcc gctttcgaag tcggtaaaac gcacctgtga ccagcacgcg
     6361 cgcaccttca ttttccattc ggcgagggct ttcgagcgcg cccggctgtt ttcctgcatg
     6421 aaaacgcgcc gctgcgttgc cggcacataa aaccggtctg tatattcctg caccatgcgg
     6481 tgggtgttga agatcgggtt gatggtccgc atggcgttct tcatgcgcat gacccagcgg
     6541 cgcggcaggt catcggaact ccgttcatag aacatcggga cgatttcctg ctcgaggagc
     6601 tcgtacagca tctcgctctc gacgacgtcc tgttcgtcgg ggtcttcata catttcgccg
     6661 cgcccgatac tccagccgtt ttcacccaga ccttcggcct cgcaccacca tccgtcgggg
     6721 atgctgatgt tgaggccgcc gttggcggcc gatttcatgc cactggtgcc tgaagcctcg
     6781 agaggccggc gtggtgtgtt gagccagcaa tcgaccccct gcaccatgta gcgggcgacg
     6841 ttcatgtcgt agtcttcgag gaagacgaaa tggttgcgca cgtcattttc gcgcatgaaa
     6901 tggatcagcg cgcggatgag gtctttgccc tggttgtcgg cgggatgtgc tttccccgca
     6961 aaaatgatct gcacgggccg ttcggggtgc gtcaggatcc ggatgaagcg ttccgggtcg
     7021 ctgaggagca gcttggcgcg tttgtacgtg gcgaagcggc gggcgaacgc gatcgtgagc
     7081 acctccggat cgagggcttc cgaagccgct tcgacctccg cgttgggcgc gccccgcgat
     7141 ttgagttgag ccgccaggcg gcgccgcgcg aagttgacca ggcgttcacg gcgccgttcg
     7201 tgggtgcgcc agagctccgc atcgggcacc tgatcaatat tcttccagac cgccgggtcc
     7261 gacggattgg agatccagcc cgggcccagg tagcggtcaa agaggcccgc aagatcccgc
     7321 gacacccaga attgctggtg gatgccgttg gtgatggagg tgatgggcac ttcgtcttca
     7381 ggcacacctg cccacgtgcg cgcccacatg gcacgcgcgg tcgcgccgtg cagctcgctt
     7441 accccgttgg cgaaggccga cagtttgagc gcgagcacgg tcatgcagaa cggttcacga
     7501 tggtcgctgg gatcctggcg tccgagcccg agaaaccggt ccatattcat gccgacgtcc
     7561 atcgcgtatt tgtgaaaata atgctccatc atttccggca ggaacatgtc attccccgcg
     7621 ggtacgggcg tgtgcgtagt gaatacgctg gcggctttca cggattcgat tgcgtgttcc
     7681 aggccgagct cttcgccttt caccaggttt ttgatccgct ggagggtcat gaaggcggag
     7741 tggccttcgt tcatgtgcca caccgtgggg tggatgtcca atttgcgcaa catcgtgacg
     7801 ccgcccatcc ccagcaggat ttcctgccgg atccgcatgt ccgtgctgcc gccgtagagg
     7861 cgcgcggtga tctcgcggtc gtccggttcg tttgcgtcaa agtcgcaatc cagcagatag
     7921 aggggcacgc ggcccacgtc gatacgccac acccgggctt tcacaggccg ttcgggatac
     7981 tccacttcaa tggtgacttg tttgccggtc tcatcccgga gcagggtgac gggcatgttg
     8041 tagaaatcgt tgaggggata acgttcctgc tgccagccgt ccgcattaag atactgctgc
     8101 aggtagcctt cccggtacat cagacccacg cccaccaggg gcaggcccat gtcgctggcc
     8161 gccttcagat gatcgccggc cagcacaccc aggccgcctg aatacagcgc cagcgattca
     8221 tgcaggccga actcggccga catatacacc gcctggaatt cctttggcgc ctgaggattt
     8281 ttctcgtgcc agctgttgcc ccggagataa gcctgaaacc gcgaatgcac ccgttccagg
     8341 tgggccatgt atccatcgtc gtgcgtcagt tcttcaaggc gttcctgcga cacctcgccc
     8401 aacaggcgga ccgggttctg tttcgccgtg atccagcggt cgcggtcaat gcggaagaac
     8461 aaatcgatgg cttcgggatc ccaagaccac cacatgttgt acgcgagact gcgcagcggt
     8521 tccagcacgg gcggcagttt cgcgacgacg gtgaattcct tgattctcgg cataagaggt
     8581 cactccacag gctcattcac acgtattgat ttggaataat gggggtgagt gtacttgttt
     8641 ttgcgttgtt agggcaagtt attgccagga cttcccctgt tactcctgtc aatcccgggc
     8701 ctcactttcc gttatccggc gctctttcca gccttcccac gtgtacgccg ggtccgggga
     8761 ggctgagccc ggaaagcagg ccagctccgc gggcacccgg tactgcgcgc cgatatcaag
     8821 cgcgtcggtg ccccgctggc gcggttccgt gtcgtagagc ggttcgagct tcagaccgtg
     8881 cgcatcgacc cagtctccga gtttgattgt cgctgcgggg agcggccgcc tatcttcggg
     8941 ggcctggagg tattcctgaa accacgccgc tgccggccgc gtgaggatgt acggccgatg
     9001 gcaggccccc gctacgaact gcgcttcaat gatgcccggt atccccgcat ccgcgagaat
     9061 ctgccgcgcg ccggccaccg tggcgcgcgt acggcggacc acggccagtc ctccttcggc
     9121 cacatcgata atggagtcct ggccgccatt gatgaacaag gtggcggcgt gtgaggcgag
     9181 cagggcgttc aattcgtcaa agctcatggc gtgggcgagg gcttcgaaag gcattttgct
     9241 gcagtctttc ccctggatcg ccccctgggc cgtcatgctc cagccgctga tcaccgccgc
     9301 gcgcacgcgc gtatcgatgg cggccaccag gctcgacgtg gtgcccccca tggaattacc
     9361 catgacgccc agccgcgccg gatcaatgtc cggccgtgtc tccaggtagt ccagcccgcg
     9421 gaccagatcc cagacgatct tccccagcac catgcggccg agtttttcgc gggtaaaggc
     9481 cctccgggcg gcgctgtctt tgccgaattc atacatatcg tgggcgcgcg tgcccatttt
     9541 gccgtcttta tggcgttcct cttcaccgat ggtatcgggg atgaagcagg caaaacccag
     9601 tttggcgtag agctggccag cgtattggta gtaaatgctg gatttcgaac ctccgtgccc
     9661 gcaggccagg acgatgcccg ggacgggcgc atccgctggc gccggcaggt acagatgggc
     9721 ggttacccgg gaatccgcct cgctttgata ggtcacgcat tcgatgcgat acccttcacc
     9781 atcaatgctg cggtgagtct gggcatccag agccacgcgt tccgcaggaa acgtcaggat
     9841 ctcgcgcacg gtttggcgca attgatccgc ctgcgcggcc caaccggtat ccaaaccggc
     9901 tggtagaggt ccctgagacg ccggtactgc ttctgccagc gcccctaaac ccagtaaaat
     9961 cggccagata atccccatca tgaaggcaat cctccccgcg tgaaagcata cacttccgga
    10021 cggcaggatg ccatgcgcct cgccaagggt caggcggttc caaacgattg gcgaacttgc
    10081 gtgatcggat ggggtcttaa agaatgcccc gctgggagcc tgtcgaagaa ctgggtttgt
    10141 ggatgcgaag aaaaagcttc acacacggcg ctggcccgcg tggccggggg tggatgaccg
    10201 gctctgagcc tccgccttcc ttcagggctc acgggcgcgc cagaagctta tgtttcgccg
    10261 ccttttcaga tcacttcgag ttggtctctg ccgggaaggc tgggaaacgg tgcgtgaggc
    10321 tcggcctggt accaccatgc taccgacgcg atgtcgtcct gcaagggcag gtaccgtccc
    10381 ccgctccgcc agcccagggc ctgtatggtc acccgcagat cctgttcaaa gcggatcggg
    10441 tccatgatgt gccatcggta gaggccaaac cgctgctgcg aacggtaggc gccgtcaggg
    10501 cggatgacct ggggcaaccc ggcataggcc gtgctgaatt ccgcatagtg gccgttgcgg
    10561 tcgaagttgt aagagccaca gaagtaatct tccgtgcccg tgccgcagat ggttgggaac
    10621 tccccgtcgc cgtcgagaaa gaatttgatc tcgccctccc cccaccagcc ggtgttattg
    10681 acttgccagg cgaggtaggt gccgacgtaa tggccgtgcc ctcggatgcc gtccgcgatg
    10741 gtataggtct ctttgtacgg gagcggattg gtccggcgga attgggcatg aaggtaggcg
    10801 cagtcgtcgg gcacgctggt gagggtgtag ttgatctggt agaacaagac cataggctca
    10861 tcgccgatat tatcaaacgt catgcagcaa tgttttcgga aaggcatttc ccagtagcag
    10921 ttgaacgcgc tgccgggatt gacgcagacc ggcagggagt tcacgtgggc atattcgccc
    10981 cagcccatgc agaaaaagtc gccgacaggc gtttcgatgc tggggaggtc ctcgccgtcc
    11041 caatagacgc gcaggatgct gaagcgccag ttgccggtgg gcgtcatcca gatctgctgg
    11101 atcgcccccg ggccgtcgat gctggcaagc tcgaaggtct gccccggttc gatgcagacc
    11161 gacggcgaga tcttccagcc ctggccgagg tcgcgcgcgc attgggcgcc cgtgccgtcg
    11221 gtagccatgc cgccgccgcc ttttgcacca tcaaagtttt ccgccgaaat cgagcgtgtc
    11281 ttcgcggttg acaggcgcga caggttaccc agatgcattc caaggccgtc gaacatgatg
    11341 tgtctcccct gtatttgatg cgcgtcctgt tgccggccgc gccgccgcgc ataataccat
    11401 gcgcagacgc aacgggcggc tcgactggcg gaataacgcg ctatcggcag cggcgcccgg
    11461 tctcaaggct gataaggcac cgtctccgtg tcatcgaccg tgtagccgaa ccgttcgatg
    11521 taggcgcggc cggggaggaa ctcgtcatta cgttgtgcga gccggacgga caactgcgca
    11581 tccagctttg cctggacctc cgccatcccg gggtcattgc accggttttg gagctgacag
    11641 gggtcgcttg cgttgtcgta gaggagccag ggaccgttga gatcgcgcac gtaggtgtat
    11701 tggccgtcga cgatgccgcg gtattcacgg ccgccgtgtt tacgcgagac ctgcccgaac
    11761 ggctgagggc acatgatgac ggcggttcca tcgccgggat tgggtccgcc gccgatgtac
    11821 ccggagtaat cgagtccttc gacggtcccg ggcacgggaa tgccgcagag gccgagcagg
    11881 gtgggcatga tgtcgggcgc atcgatcggg gcgtcaagct ggcggccctg tctcccgaac
    11941 gcggcgggat accgcagcag gaagggcacg cgaatggact cctcaaaagg ccactgcttc
    12001 ttccagacac cctggcagcc cagcatgtcg ccgtggtccg aggcgaacac gaaaacggtg
    12061 tcctcggcaa taccggcctc ggcgagggtt tgatcgagcg cggcaaggca gtcgtcaagc
    12121 gcggctatgt gagcatagta acccgcgaga tcggtttggg cctgctcctg cgcctccgga
    12181 gggacattgg gccgcagcac gatatcctca gggcggaatc gctgacggta gggttccggg
    12241 gcagtctggt agggttcgtg gggtggcccc caggagagaa agagcacgaa cggcttctcc
    12301 gcccgcgcat gagcgcgaat gtattgctgg gcgtcccggg tctgggcaat ggcgtcgtat
    12361 ccgtcccaca gcagtttctc gtctgcatca gcatagtagg cggaatgatt gtagtcgtgg
    12421 gtacattcga gcaccttcca gtagtcccat ccctggcggc gctcgggagg gatgtagctt
    12481 gaacggccgc ggccgccgag atgccatttg ccgatataag ccgtgtcata accggcgttt
    12541 ttgaacactt ttccgatggt aacggcctcg ctgggcagcg gcacgtcgtt catgaacacg
    12601 ccgttggtca ggggcgtctg gcccgtcatc aggctggcac ggtaaggcgt gcagacgggg
    12661 cagcccgaca cggcgcgggt gaagttcacg ctttcggcgg cgagccgggc gagatacggg
    12721 gcctgcacat tcgggtcctt gttgaagggg gtggcctggg cgcgccactg gtcggcaaaa
    12781 acgaaaacga cgttgggttt cgataccgga gattcggtcc ccgcgtgcgc ggcatgggcc
    12841 agcgcgacgc cggccgccga accggccaga aaatggcgcc ggctcatggc tgttctcgag
    12901 tttgttcgcg gctggttcat aatgacttac tcctccccgg ccatggtaga gaggagtcca
    12961 gcaaactgca aggcggggtc gtggggccgc aatgaggctg gccattccgg ctgcgatcta
    13021 ggcgtcggca ggaaattccc ccagctgccg ggcctcgccg gcgaatacct cccgctggat
    13081 gcgtttaacg gaggcatcgt aatcccattc ggaagacagt ggtttccatc caaggctgtc
    13141 cgccattttt cctgcaaacg atgcgaccat tccccaggga tcttcgatgc cgagggcttc
    13201 cagagacacg ccgctgagaa cgatgccgta accgccgtcc gccagggccc ataaggccca
    13261 cacgattctg ccaaccgtgg tgccggggcg gagcaccaga tctccgtcgg tcacggcctg
    13321 atgcaccatg cgcatgagat aattgatgat acgctgctcc agtttttcaa aacgggcgcc
    13381 ccgctcgggc gagatctttt cgcggaacga acgggtgatg gtcttcatga gctgcatgtc
    13441 gcccgggttg agacgcgcgt aatattcgcc ggccagacag aaggccagca agcgttcccg
    13501 gctgcacccc cggaaaccca tggcgcgccg gagcatggca tagcgctctt cgtgcgcccg
    13561 ctcggcgagc gcgacgatga tgtcctcctt gcttggaaaa tggagataca cggtcccttt
    13621 ggcgcagttg atccgtttgg cgattttgcc catggtgagg ttgtagtatc ccgcttcgaa
    13681 aaacatctgg cgggcagtct ccaggatatg ttcctctcgc tcccggatct cgcgctgttt
    13741 cggcgtcagt ccgtctccag cacaaatcac acgctttcct ccgcttgccg ggcgtaagag
    13801 gcgctccttg ccactttttc cggctcactg cgcgtatcag atagaaatga gtacagcctg
    13861 gttgcagtca cttcactaac cacactgtgc ctggcagaat attagtatat gctctgcgcc
    13921 tctgttatac gcaaattcag tgcccggcat ttcggcgcca gcggtctcgc ccgggcacgt
    13981 atgcccgggc cggagcgcta ggggagatgg acgagctcga cggccacgtc tttcgggtta
    14041 ggtccgggat ccatacgcgg ggcatgggga gagaccacaa acagcgtgcc ggacacattg
    14101 cgcgcaccga attcagtctc atgttcgcgg atggcaagca cgggccggtc gacggtgtca
    14161 tagacataac aggcgtcgaa ggccacgccc ccttgctcgc gggtgatctg ctgccggcga
    14221 agctcgatct ccagtggaga ccgcggaaca tcggcctgtt tgcccggttg cgaccaggag
    14281 ccgctccaat ggcagttcgt gaagcggatc tccacacttg acgctgcttt gtcgtaaatg
    14341 cgcaaaccgt gctcgtgagg gttttcgacg gtgcaattgc ggaactcgac ttgcccgccg
    14401 ggcccctgat cgttcaccgc gcctatcaca atgccggaac tgttgccggc accgacgaag
    14461 cagtcttcaa aagtcaggga tacggggctg ctgccggcgt cgaggttttt gaggtacagg
    14521 agtatgccgg cgccctgatt gccggagaaa acacaattgc gcaccaggca gttgacaagc
    14581 ttttcgcggg ggtggttggg ctcgaaatcg atgcctgcag caggggctgt cccgctggta
    14641 tcggtcatca cggtgttctc gataagcaaa ttcaccgctc cgatgacgct gatgccctgg
    14701 cggtgatggt cgtggcagat cacgtcgcga atgacgacat cctcgcacca ggccagttcc
    14761 tgcgtcgccc ccaggtagat cccatctcct ccgctgcctt caagacgaag cccctcgacc
    14821 cggatgttcc ggcagccgcg aaacgccagg cccatccgcc attcagcttt ttcataggcg
    14881 tcgctctggt aatccgcctt gcgcatacgc aaggtcgccc catagccgcg gaaagtgatg
    14941 ttggcggcgt tcgctgcggt aaaaaggctg tcgctctttc ctttaaactg gccttcctt
//
LOCUS       DAOPBQ010000021        14409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4238_length_14409_cov_4.607218, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000021 DAOPBQ010000000
VERSION     DAOPBQ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 14409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14409
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4238_length_14409_cov_4.607218"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1235
                     /gene="hemW"
                     /locus_tag="PKW60_01550"
     CDS             123..1235
                     /gene="hemW"
                     /locus_tag="PKW60_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPJ98145.1"
                     /translation="MIGVYIHVPFCRTLCPYCDFYRVRLDGPVPDLFVDALVREIDAF
                     EADKEADAIFAGGGTPSLLAPGQLERILDALRTRFDLWEPEITLEANPDDVTEALADA
                     WLALGINRVSLGVQSFDDAALRYLGRRHDAATARRACEIVAGRFANWSMDLIYGAHPT
                     SSWAATLAECVSLAAPHVSCYGLTFEPSTPFGERAHEAIGEDEELTCYRACSEQLAGY
                     QHYEVSNFARPGFQCRHNLYYWRNGEYAGFGPGSFSFIDGVRSCNESDLSAYILEPGR
                     KAERLVLSEREIRVETLIQHFRLREGLSRDVYAQRFGRDLNADFGAALQTLVQRGLLK
                     ERGNRYLPTKSGFELNNEIGLVLVDNPGEGPWPAAN"
     gene            1362..3086
                     /locus_tag="PKW60_01555"
     CDS             1362..3086
                     /locus_tag="PKW60_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPJ98146.1"
                     /translation="MFRHTAILLILGALYPLALAQGEDAPPHPDSDYGYIIVCTLDGE
                     VDEAMAVFVERIADYAEDAEGVVFIIDTPGGRVDSGLEVTKAIQHAGKFCPTIAYITD
                     GFSASSAGALVAYACKHIVMTSSATIGAAKPIMLTPQGALPGDEKTVSFLRAKFSALA
                     ESNGHNPDLAMAMVDEDIELRAFKVGTKVLVKAVDGLPRSTASRKTDALEQILDYVDR
                     RLPLPEELKDAARDLAGEAVGDAESENGTAAVPLSLADAERYGGPGNPSETYPPEGKL
                     VSASGKLLALTASEAREYGLIPDIFDDLEHVLSFFGLSQYERFEYEMTWPEILFRWLT
                     SPTVSSLLLLFGIGGLYLELKTPGFGAPGIAGITCLVIFFGARAVLGLANWLDIALVL
                     VGLGLILLEIFVIPGFGATGVAGIICVVLGIVLSFLLNDWQLPQYQWDYERLTDAAYS
                     LGLSMGLFLIFVAVTWKILPRTPLYGRLVLADEQRAEAGFVAQTDQEEEAAIGLQGVA
                     TTMLRPAGRGRFGATTLHVVSRGEFLAPGTPIVVTQVEGNRYVVAPMARDKDNIPREK
                     RSPWISGN"
     gene            3068..3370
                     /locus_tag="PKW60_01560"
     CDS             3068..3370
                     /locus_tag="PKW60_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98147.1"
                     /translation="MDKRQLIIHMLRQLDEQSLSIQQVGAGYYSCIPFARRFNKLLSE
                     ARTLFTATDGLIGTFEEIAEFDPKDPGDKMKIIQGIRVEINQLIALLEAAGEDGGQ"
     gene            3367..3864
                     /locus_tag="PKW60_01565"
     CDS             3367..3864
                     /locus_tag="PKW60_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPJ98148.1"
                     /translation="MIWWVVILFAAGAGLITVEFVVPGGICGTAGALLIIVSAVMGCY
                     HYPDYWPPILLLEFLGTICCVLLGIYLLPRSPFGKLLMLGDAQKADEGWVNETGNTEL
                     AGKTGTVVTPLRPAGTVEFGTERVQAVSEGAFIEAGAVVKVIEVHGNRIVVERAEPAR
                     INVDA"
     gene            3942..4952
                     /gene="floA"
                     /locus_tag="PKW60_01570"
     CDS             3942..4952
                     /gene="floA"
                     /locus_tag="PKW60_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712838.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HPJ98149.1"
                     /translation="MPMAIMWSVALFLGVIALVFFAIFIKFIGLWIRAFASGAHVGVP
                     SLLGMWLRKVNASVIVDSRIMAVKAGLGISTNDLETHYLAGGNIVRVVQALIAANKAN
                     IALSFQKATAIDLAGRDVLDAVQTSVKPKVIDCPDPTKGPPTVAAVAMDGIQLKAKAR
                     VTVRTNINTLIGGATEETIIARVGEGIVTTIGSAASHKKVLENPDSISKVVLARGLDA
                     GTAFEILSIDIADVDVGENIGAQLQTMQAEADKNIAQARAEERRAMAQAREQEMRAQV
                     VEMRARVVQAEAEVPKAIAEAFRTGNLGVMDYYRMKNIMADTDMRQSISGVESKKDDK
                     NA"
     gene            5019..5666
                     /locus_tag="PKW60_01575"
     CDS             5019..5666
                     /locus_tag="PKW60_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98150.1"
                     /translation="MDELGSLLIVIIVVVASLIGKIQEQRKASEHKRSRKPVRPEDLP
                     EATRRMLYGAPEKPPAATRREPAPVRTAQPRVAPQAARPQVVRPQAVQPPAERRPVAP
                     QHPRPMLETAPPAAPPPKVQRRPAPQPEMLSMQARHERMMAQRMKELRDAQAQRSAML
                     HRAQQIRQQGAAAVAARPRTLKKAAPFREDVFSDLDDVRRGIVMMEILGAPKAFE"
     gene            complement(5685..6500)
                     /locus_tag="PKW60_01580"
     CDS             complement(5685..6500)
                     /locus_tag="PKW60_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPJ98151.1"
                     /translation="MRASGNADVPVGNINKKKCADEDVGAPRVGVPSGRREWYSRGYL
                     PHCDAADLIQFVTYRLADSLPQTKLAELHEELRGLPENERKIARRQRIEQWLDAGMGC
                     CALAHPDVAQYVQHSFLHYHGERYHLHAWCIMPSHVHVLVEPLIELAVIVQGWKSYTA
                     RWVLKKKKQLAVKVPGANRLWMREYWDRYIRDARHYQRAVEYIHDNPVKAGLCDVAEA
                     WRWSSAFPGNADVLVGNTGNADVLVGNTGNADVLVGIRKNQADGDVGAPRSVT"
     gene            6898..8577
                     /locus_tag="PKW60_01585"
     CDS             6898..8577
                     /locus_tag="PKW60_01585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPJ98152.1"
                     /translation="MEVQRTNPLIVQSDRSILLETDGPRFEDARDAIAPFAELIKSPE
                     HIHTYRVTPLSLWNAAAAGMGAEEMIAHLKEYSKYEVPENILYEIRDTVARYGRLKLY
                     KREDGMLVLESDDALLVTQLKRQKSLQPFIEGLEDGRRVIVKALERGNLKCALIKLGF
                     PVEDLAGYVQGAPLEFELREKTLGGKEFSLRNYQLEAVDAFYAGGSNRGGSGAIVLPC
                     GAGKTVVGIGAMHRLQTQTLVLTTNTVALRQWKSELLDKTTLGDEDVGEYSGETKQIR
                     PVTMATYQVLTYRRSKDSDFEHFSLFDQGNWGLVIYDEVHLLPAPVFRATATLQARRR
                     LGLTATLVREDAREDDVFSLIGPKKYDVPWKVLEKQGWIATAACTEVRIPLPDDERYR
                     YAIADKRGKFRIASTNPIKASICRTLIERHRGDNVLIIGQYLDQLRALAKTMKAPLIT
                     GQTANKKREALYDAFRRGEQRLLVVSKVANFAIDLPDANVAVQVSGTFGSRQEEAQRL
                     GRILRPKEDGSIAHFYSLVSRDTCDQDFSVKRQLFLTEQGYHYEIKDYEEL"
     gene            8580..11723
                     /locus_tag="PKW60_01590"
     CDS             8580..11723
                     /locus_tag="PKW60_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPJ98153.1"
                     /translation="MGAVDVLAGPEGSARTERLDRLLAAQWGHALLVTPTHLFARKRL
                     EELLVRPDIAGAWGTPVSTLQTFAETILQDADPEALPLNDLERRLVLQEALQRLRAAG
                     ELENAGPGSETGGFLRHLLRVFENLKQAAVEPEEFLQCVRRRRHHAALDLVVAHAYQE
                     YQAALIERQAYDRIGVYWQARQLLEGEGDLPALRGIELVALDGFDDFTESEFRLVAAL
                     KNRVGRLVFSMRADEAAGGADLYRLQRQTLARLETAFQPTIEWLPARPPATACQHAAA
                     YLLWRDDIPPVDGLAPNLEVVPCAGVAEEVETIGRAVKTLILEGVPAAEIAIVYADLE
                     QARNALRSMGREFQIPLRVHGDEPLASSSVCAFVLDALEASDGWKHGEIVDLLTAPWM
                     DTDGADETLTGAIPVVCRQAGIVAGHEQWVKRLRRMRAELSRAPRDREFDAGQLLRRH
                     PRLLDAVITAEARMKRLSRALEMFPEADELHRFCRATAHFLDALELGKAVRTRAPESI
                     RVREIAALEALQELLGRLDARQFSTGALQTRGDFVRFLRQTCGEQTFHAPETPRERRG
                     VRCLELRSVRGLRFRYVFAGGLNEGIVPGQPGVNAVYSEDDVRELRDAGIPLESREVR
                     TERELAAFHHVLEAAQDRIWLFWRTATPDGKPLLRSPFLNDLLRLFAAKPVERTPLRA
                     SQYYPALSHAASHRDAINNLFATGTRLPEALAAEFAAVVRGADIEKRRYSLAVFDAFD
                     GVLCEERNQAETAERFGEDHVFSATELEYYRQCPFRFFALRLLRLMPAERPVTAFDRG
                     LLGIVVHQVLEKFHQHYAGKPVSEIPVREAEEAMRGVVDSVFDENSAAMITVPDGMLM
                     AERMAVHERLQRYLTIARREEVSWRPTEFEAVFGGTREKEYPPLAILTEAGILRMRGR
                     IDRIDQGGAGCRVVDYKTGGLPAPAQVKEGISLQLVAYALALENVIAPGTVCEEGVLL
                     KVGTTDRLAVRESGRGTLWEEAKKGFFEGVSAALRGIRGASFPPVPQPGVCASCDARR
                     MCRIDMSRVERKQGGAP"
     gene            11720..>14409
                     /locus_tag="PKW60_01595"
     CDS             11720..>14409
                     /locus_tag="PKW60_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPJ98154.1"
                     /translation="MKWTDAQAEAIQTTGEDLCVTASAGSGKTAVLVERVLHLVIECN
                     VPLERIVAITFTEKAAAEMKDRLRSECRAREQSGSKEGLDRWRNLARQVESAHMSTIH
                     AFCARLLRQYALEYRITPPLDPDFAVVDDAESRLLRTEVVDTAIEELLERPHDATMRL
                     ATHYGVYQLKEMLERFLRNPVTASRLLSNEQFSSPERLLKFWRVRVEETCRARLLNLS
                     KSAVFYNYLRKFKEFDGRCGIPTDGREQFRRTCIQLMEEVGQLRDPLLIEQLIMACIE
                     ERIPTTRKANWPSEEVFEELKKTQEAFKGYLREFLMPDHDPDVEQRSAELTRDAVACF
                     RAVFEYYREAKTSRNVLDFTDLILLTLDMLQGQPELRERIARGIDHLLIDEFQDTDFE
                     QYEIARLLRYTPAPPELFIVGDAKQSIYRFRGAEVEVFEAAKQESRTIGLHRNFRTVP
                     EIVSFINDIFDRSGILEAVESPFMPADAHRAATGECRVHVLIPDAPEDKELSAQRRMR
                     EAAMIGDWIAAAHSGQLDVQVQAKGSDTTRKAAFGDMALLFRALSDIRIYERALAERH
                     IPFHVIAGKGFYERQEVLDLRNLLEAVVDPWHEPALLGFLRSPMAGLDDDSILAICAG
                     PGPVAALLEDGTPPDFRQTAGLDHARRMFRDLRAERERPLPDFVRYVLERTGYEAMVA
                     GLFLGDQRACNVRKVMQLAETFVKTRRPRLSAFVRYLDDVASAEIDEGEASLQAEGSD
                     EVTLMSIHKSKGLEFPIVILPDLSRGSHQGGNVGRVVFHRDFGIVARPYNPASERQTE
                     PQIYTVMKRDEEAKEEAERARVLYVAMTRARDWLVLSGTVASGKSPRPAPDSLLEPIA
                     SELDVLNRGDGALLSGEGWTALVRRSTTPAAIDAPAPEPAL"
BASE COUNT         2748 a         4170 c         4701 g         2790 t
ORIGIN      
        1 cggcgatctt cttcacgagc tgtccagcca tgcaggtcga gcattttgac aggctgatgg
       61 cgggagaata gaaatcggca cgcaaagcgg cggcgcgtgc cgtgccaggg agtgatccgc
      121 gggtgattgg cgtctatatt catgtgccgt tttgccggac cctgtgtcct tactgcgatt
      181 tctaccgcgt acgccttgac ggtcctgtcc ccgacctgtt tgtcgacgcg ctggtgcgtg
      241 aaatcgacgc tttcgaggcg gacaaggaag ccgatgcaat ttttgcgggc gggggaacac
      301 catccttgct ggctccgggg caactcgaac ggatccttga tgctctccgg acgcgctttg
      361 acctgtggga accggagatc acgcttgaag ccaatcccga cgatgtcacg gaggcgctgg
      421 ccgacgcatg gctggcactg ggcatcaacc gggtgagcct gggcgtgcaa agcttcgacg
      481 atgctgcgct gcgctatctt ggccggcggc acgatgctgc aacggcgcgg cgggcctgtg
      541 aaatcgtagc cggccgtttt gccaactgga gcatggacct catatacgga gcgcatccca
      601 cgtcttcctg ggcggcgacg ctggcggagt gtgtgtctct ggccgcgccg catgtgtcgt
      661 gttatgggct gacatttgaa cccagcacgc cttttggaga gcgggcccat gaggctatcg
      721 gggaggatga ggagctaacc tgctaccggg cgtgcagcga acaattggct ggctatcagc
      781 actacgaagt atccaatttc gccaggcctg gctttcaatg ccgccacaac ctttactatt
      841 ggcgcaacgg cgaatatgcg gggtttggcc cgggctcttt ttccttcatc gacggcgtgc
      901 gctcctgcaa tgaatcggat ctgagcgcgt atattctcga gcccggccgc aaggcagagc
      961 gcctggtctt atcggagcgc gagatccggg tggaaaccct cattcagcac ttccggctcc
     1021 gtgaggggtt gagccgcgat gtgtatgcgc agcggttcgg gcgcgattta aacgcggatt
     1081 tcggtgcagc actccagacc ctggtacagc ggggcctgct taaggagcgc ggaaaccggt
     1141 acctgccgac gaaatccggt tttgaactca acaacgagat cggcctggtt ctggtggaca
     1201 atccgggaga agggccgtgg ccggcggcga attgaaccgg ccgtggcgtg agtctagaat
     1261 gagatccgcg gcccgatgcc ggtagcggcc aatataaggc gtgttgcggg ccgcgaatcc
     1321 ccgcgcgggg gatggccgcg ctcgtacgag gtggcgcgag catgtttaga cacactgcga
     1381 tcctgctgat tctgggggcg ctgtatcccc ttgcgctcgc gcagggggag gatgcgccgc
     1441 cccatccgga cagcgattac gggtatatca tcgtctgcac gctggacggg gaagtcgacg
     1501 aagcgatggc ggtatttgtc gagcgcatcg ccgattatgc cgaagacgct gaaggggtgg
     1561 tgttcatcat cgatacgccg ggcggacgcg tggactccgg tctcgaggtc accaaagcca
     1621 tccagcatgc gggcaagttt tgtccgacca tcgcctacat tacggacgga ttcagcgctt
     1681 cgtcggcagg ggctctcgtc gcttacgcgt gcaagcatat tgtgatgacg tccagcgcaa
     1741 ccattggcgc cgccaaaccg atcatgctca ctccgcaggg agcgctgccc ggggacgaaa
     1801 agacggtgtc gttcctccgg gcgaaattca gcgcgctcgc cgaatccaac gggcacaatc
     1861 ctgacctcgc catggcgatg gtcgacgaag acatcgaact gcgcgccttt aaagtcggaa
     1921 ccaaagtcct ggtgaaggct gtggatgggc tcccgcgttc cacggccagt cgaaagacag
     1981 acgctctcga gcaaattctt gactatgtgg accgaagact cccgttgcct gaagagctga
     2041 aagatgctgc acgggacctg gcgggcgaag ccgttgggga tgcggagtca gagaacggga
     2101 cagcggcggt gccgctctcg cttgccgatg cggaaagata cggcgggcca ggaaacccct
     2161 ctgagacgta tccccccgaa ggcaagcttg tcagcgcctc cggtaaactg ctcgccttga
     2221 cggcgtcgga ggcgcgggaa tacggtctga tccctgatat tttcgatgat ttggagcatg
     2281 tcctttcatt tttcggcctg agtcagtatg agcgctttga atacgagatg acctggccgg
     2341 agatcctctt ccggtggttg acgagtccca cggtcagcag cctgttgctg ctgttcggta
     2401 ttggcggact ctacctcgag ctcaagacgc cggggtttgg cgcgcccgga attgcgggca
     2461 ttacctgcct ggtgattttc ttcggggccc gcgccgtgct cgggctggcc aactggcttg
     2521 acatcgcgct ggttctcgtc gggctgggct tgatactgct cgagatcttc gtcatcccag
     2581 gcttcggggc gacaggcgtg gcggggatca tctgtgttgt gctgggtatt gtcctgtcgt
     2641 ttcttctgaa cgactggcag ctgccccagt accaatggga ctatgagcgg ctcacggatg
     2701 ccgcgtattc cctgggttta tcgatgggtt tgttcctgat ttttgttgcg gtgacgtgga
     2761 aaatcctgcc gcgcacgccg ctgtatggcc ggctggtgct ggcggatgaa cagcgggccg
     2821 aggccgggtt cgtcgcacag accgaccagg aggaagaagc ggcgattggg ctgcagggag
     2881 tagcaaccac gatgctgcgt cctgccggcc ggggacgatt cggggccacg accctgcatg
     2941 tggtcagccg gggcgagttt cttgcgcccg gcacgcctat tgtggttacc caggttgaag
     3001 gcaaccgcta cgtcgttgcg ccgatggcgc gggacaagga taatattccg cgggagaaga
     3061 ggagtccatg gataagcggc aattgatcat acacatgttg cggcagctgg acgagcagag
     3121 cctcagcatc cagcaggtgg gcgcgggata ttacagttgc atcccgtttg cgcggcgctt
     3181 caacaagctg ttgtcggagg cgcggacgct gttcacggcc acggacgggt tgatcggtac
     3241 gtttgaagag atcgcggagt ttgatccgaa ggatccgggg gacaaaatga agatcatcca
     3301 ggggattcga gtggagatta accagctcat tgcgctgctc gaggccgctg gggaggatgg
     3361 cggccaatga tctggtgggt ggtcattttg tttgccgcgg gagcggggct tatcacggtg
     3421 gagtttgtgg tgcccggcgg gatctgtggt accgcggggg cgctgctgat cattgtaagt
     3481 gcggtgatgg ggtgctacca ttacccggac tactggccgc ccattttgct gctggagttt
     3541 cttggaacca tctgctgcgt gctccttggg atatacctct tgccccggtc gccatttggc
     3601 aagctcctga tgctgggcga cgcgcagaag gccgatgaag gctgggtcaa cgaaaccggc
     3661 aataccgaac tggccggcaa gactggtacc gtggtcacgc cgctgcgtcc ggcgggcacg
     3721 gtcgaatttg gcaccgaacg cgtgcaggcc gtatcggaag gggcgtttat cgaggcgggc
     3781 gccgtggtaa aagtcatcga ggtgcacggc aaccggatcg tcgtcgaacg ggcagagccg
     3841 gcgcgaatca atgtggatgc gtagcttttg aagagtatcc tgcttcagca gaatctggtc
     3901 atttccacag accggctgcc ggtctgagaa agggggtcgt tatgccgatg gcgattatgt
     3961 ggagcgtagc gctgtttctg ggcgtgatcg cgctcgtctt tttcgcgatt ttcattaagt
     4021 ttatcgggct ctggatccgg gcgtttgcgt ccggggcgca tgtcggcgtt ccaagcctcc
     4081 tggggatgtg gttgcgcaag gtgaatgcat cggtcattgt ggacagccgc atcatggccg
     4141 tgaaagcggg cttggggatc tcaacgaacg atctcgagac ccattacctg gcggggggca
     4201 acatcgtccg ggtcgtgcag gcgctgatcg cagccaataa ggcaaatatc gcgctgagct
     4261 tccagaaagc gacggccatc gacctcgcgg ggcgcgatgt gctcgacgcg gtccagacct
     4321 ccgtcaaacc caaagtgatt gactgcccgg atcccaccaa aggcccgccg accgtggccg
     4381 ccgtcgcgat ggacggtatc cagctcaaag ccaaggcgcg ggtaaccgtg cgcacgaaca
     4441 tcaacaccct gatcggcggc gcaaccgagg agaccatcat cgcccgtgtc ggcgaaggta
     4501 ttgtgaccac gattggttcc gcggcatcgc acaagaaagt gctcgaaaac ccggattcta
     4561 tctcaaaggt ggtgctggcc cgcggcctgg atgcgggtac ggcgttcgaa atcctgtcaa
     4621 tcgacattgc ggacgtggat gttggcgaga acatcggcgc acaactccag accatgcagg
     4681 ccgaagctga caagaacatc gcgcaggcac gcgcggaaga gcgccgtgcc atggcgcagg
     4741 cgcgcgagca ggaaatgcgc gcgcaggtgg ttgagatgcg cgcccgcgtc gtgcaggctg
     4801 aggcggaagt gcccaaagcc attgccgagg cgtttcgtac gggcaatctg ggcgtcatgg
     4861 actactaccg catgaaaaac attatggcgg atacggacat gcgccagtcg atctccggtg
     4921 tggagagtaa aaaagacgat aagaacgcgt aagcgaagcg cgcccgccgc cccgccgatg
     4981 cgggcagccc tctggtttca ggggcgaaag ggttctggat ggacgaactc ggcagtctgc
     5041 tgattgtcat catcgttgtc gttgccagcc tgatcggaaa gatccaggag cagcgcaaag
     5101 ccagcgagca caagcgcagc cgcaaacctg tccggccgga agatctgccg gaggcgacac
     5161 ggcgcatgct ctacggtgcc cctgagaagc ccccggcggc gacgcgtcgt gaaccggcgc
     5221 ctgtccgcac ggcacagcct cgcgttgcgc cccaggccgc gcggccgcag gtcgtacgcc
     5281 cgcaggccgt acagccgccg gccgagcgcc ggccagtcgc gccacagcac ccgcggccga
     5341 tgctggaaac ggcgcccccc gcggcgccgc cgcccaaggt ccagcgccgg ccagcgccgc
     5401 agccggaaat gctctcgatg caagcgcgcc acgagcgaat gatggcgcag cgcatgaaag
     5461 aattgcggga cgcccaggcc caacgctcag ccatgcttca ccgtgcgcag caaatccggc
     5521 agcagggcgc ggcagccgtg gcggcgcgcc cccgaaccct caagaaggcc gcgccattca
     5581 gggaagacgt gttctcggat ctcgacgatg tccgccgcgg catcgtcatg atggagatcc
     5641 tgggagcgcc caaagccttc gagtgatcgc caccggccgt tcagtcatgt aacactcctg
     5701 ggagcgccga cgtccccgtc ggcttgattc ttcctgatgc cgacgaggac gtcggcgttc
     5761 ccagtattgc cgacgaggac gtcggcgttc ccggtattgc cgacgaggac gtcggcgttc
     5821 ccgggaaagg cgctcgacca gcgccatgct tcggcgacat cgcagagtcc ggctttcacg
     5881 ggattgtcat gaatgtattc gacagctctt tggtaatgcc tggcgtcacg aatgtagcga
     5941 tcccaatact cccgcatcca caggcgatta gctccgggga ctttcaccgc gagctgcttc
     6001 ttctttttca atacccaacg cgcggtatac gatttccacc cttgcacaat cacggcgagt
     6061 tctatcaaag gctcgaccag cacatggaca tggctgggca tgatacacca ggcatgaagg
     6121 tgatagcgct cgccatgata atgcagaaaa gaatgttgga catactgcgc cacatccgga
     6181 tgcgcgaggg cacagcagcc cattccagca tcaagccatt gctcgatgcg ctgccggcgg
     6241 gcaatcttcc gctcattttc agggagcccc ctaagttcct cgtgcagctc agccagcttt
     6301 gtctgcggaa gactgtcggc aaggcgatag gtcacgaact ggattaggtc tgcggcgtca
     6361 caatgcggga ggtagccacg cgaataccac tccctgcgac cgctgggaac gccgaccctg
     6421 ggagcgccga cgtcctcgtc ggcacacttc ttcttattga tattgccgac ggggacgtcg
     6481 gcgttcccag acgctctcat gctccttcct cctctggcgt ggggaaaagc tgttgtatca
     6541 ggccgcgctt cgctgattcc gccttcttct tcacgatatt tggtctcatc aagttgcaaa
     6601 ttagaataat actattgatc atttgtaaag aagtttccaa ctcgaagagc cttgtatttc
     6661 ctctcgccgg gaagcccctc tttttcataa actggtccgt tgaagaatgt cgcggccaga
     6721 gcgagtttct tgcgcggtcg cgcgtgcaca ggcccgctac attcacgcct tgccttttga
     6781 tacaatcgtt gggatacgcg cgtggcctca ggttatttgc gcgaggttcg caggctctct
     6841 tcaggggagg ggaccccgcg gcgcacatca tacgcgcggc cgatatcgga aagggaaatg
     6901 gaagtccagc gcactaatcc gcttatcgta caatccgacc gctccatact gctcgagacc
     6961 gatgggccgc gtttcgagga tgcccgggac gcgatcgccc cgtttgcgga attgatcaaa
     7021 tcgcccgagc acattcacac ctaccgggtc acgccgctgt cgctgtggaa tgccgcggcg
     7081 gcaggcatgg gcgccgagga gatgatcgcc cacctcaagg aatacagcaa atacgaggtg
     7141 ccggagaaca tcctgtatga aatccgggat accgtggcgc ggtatggccg tttgaagctg
     7201 tataagcgcg aggacggcat gctcgtgctg gaatccgacg acgcgctgct ggtgacgcag
     7261 ttgaagcggc agaagtcgtt gcagccgttt atcgaagggc tggaagacgg ccgccgggtg
     7321 atcgtcaaag cgctggagcg cggcaatctg aaatgtgcgc tgatcaagct cgggtttccc
     7381 gtcgaagacc tggcgggcta tgtgcagggc gccccgctcg agtttgagtt gcgcgagaag
     7441 accctgggcg ggaaggaatt ttcgctgcgc aactaccagc tggaggccgt cgacgcgttc
     7501 tacgcgggcg gctcgaaccg gggaggcagc ggcgcgattg tgctgccgtg cggcgcgggg
     7561 aagacggtgg tgggcattgg cgccatgcac cgccttcaga cgcagaccct tgtgttgacg
     7621 accaataccg tggcgctgcg ccaatggaag agcgaattac tcgacaaaac gacgcttggg
     7681 gacgaagacg tcggcgaata cagtggcgag accaagcaga tccggcccgt gaccatggcg
     7741 acctaccagg tgctcacgta ccgccggtcc aaggacagcg acttcgaaca tttcagcctc
     7801 ttcgaccagg gcaattgggg cctggtgatc tatgacgagg tgcatcttct gccggctccg
     7861 gtatttcggg cgacggccac gctgcaggcc cggcgccgcc tgggtctgac cgccacgctg
     7921 gtacgcgaag atgcccgcga agacgacgtc ttcagcctga tcggccccaa aaagtacgat
     7981 gtgccctgga aggttctcga aaaacagggt tggatcgcca cggccgcctg caccgaagtg
     8041 cgcattccgc ttcctgatga cgagcggtac cgctacgcca tcgccgacaa acgcggcaag
     8101 ttccgcatcg catccacgaa ccccatcaaa gccagtatct gccggaccct gatcgaacgg
     8161 caccggggcg acaatgtgct gatcatcggc cagtatctcg atcagctgcg ggcgctcgcc
     8221 aagacgatga aagccccgct catcaccggg cagaccgcga acaaaaagcg tgaagcactg
     8281 tatgatgcgt ttcggcgggg agaacagagg ctgctggtgg tgtcgaaagt cgccaatttc
     8341 gctatcgatc tgcccgatgc caacgtcgcg gtgcaggtgt ccggcacctt tggctcccgt
     8401 caggaagagg cgcagcggct cgggcggatt ctcaggccca aggaagatgg ctcgatcgcc
     8461 catttctatt cgctggtctc gcgcgatacc tgcgaccagg atttcagcgt gaaacggcag
     8521 ctgtttctca cggagcaggg ctaccattac gagatcaaag actacgagga gttgtagcca
     8581 tgggggccgt tgatgtgctt gctggaccgg aggggtccgc gcgcacagag aggctggatc
     8641 gcctgctggc ggcgcagtgg gggcatgcgc tgctggtgac cccgacccac ctgttcgcca
     8701 ggaaacggct cgaagaattg ctcgtcaggc cggacattgc aggcgcctgg ggcacaccgg
     8761 tgagcaccct ccagactttc gcggaaacga tcttgcagga cgccgatccc gaggcgctgc
     8821 ccctcaatga tttggagcgg cggctcgttc tgcaggaagc gctgcagcgg ctccgtgccg
     8881 ctggggagct tgaaaacgcc gggcccggca gtgaaaccgg aggatttctg cgccatctcc
     8941 tgcgtgtatt tgagaatctg aaacaggcgg ccgtggaacc ggaggaattc ctgcagtgtg
     9001 tccggcggcg caggcatcac gccgccctgg acctcgtggt tgcgcatgca taccaggagt
     9061 atcaggccgc gctcattgag cggcaggcgt acgaccgtat cggtgtgtat tggcaggccc
     9121 gccagcttct tgaaggggag ggggacctgc cggcgctccg gggtatcgag ctggtggctc
     9181 tggatggatt cgacgatttc accgagtctg agttccggct tgttgcggca ttgaagaacc
     9241 gggttggccg tctggtgttc tcgatgcgcg cggatgaggc ggctggtgga gcggatctct
     9301 atcgccttca gcggcagacc ctggcgcggc tcgaaacggc gtttcaaccc accatcgagt
     9361 ggctgccggc gcgcccacct gcgaccgcct gccagcatgc ggcggcctac cttctctggc
     9421 gcgatgacat tccgccggtg gatgggttgg ctccgaatct tgaagtcgtt ccctgcgccg
     9481 gcgtggccga ggaggtcgaa acgatcggcc gggccgtcaa aaccctgata ctggagggag
     9541 tgcccgcggc cgagattgcc atcgtgtacg cggacctgga acaggcccgg aatgcgctgc
     9601 gttcgatggg gcgggaattt caaattccac ttcgcgtgca cggcgatgag ccgctcgcgt
     9661 caagttcggt gtgcgcgttc gtgctggatg cgctcgaagc gtcggatgga tggaagcacg
     9721 gcgagatcgt ggatttgctg accgccccgt ggatggacac ggatggtgct gacgagacgc
     9781 tgacgggcgc gatccccgta gtctgccggc aggcgggcat cgtcgccgga cacgaacaat
     9841 gggttaagcg gttacggcgg atgcgcgcgg agttgtcgag agcgccgcgg gatcgagagt
     9901 ttgacgccgg ccagcttctg cggcgccatc cccgcctgtt ggacgcagta atcacggccg
     9961 aggcgcgcat gaaacggctg tcacgcgccc ttgagatgtt tccggaggcg gatgaactcc
    10021 acagattctg ccgtgcgacc gcccatttcc tggatgcatt ggagctgggc aaggccgtgc
    10081 gaacgcgcgc gccggaatcc atacgggtgc gggaaatcgc ggcgctcgag gcattgcagg
    10141 aattgctcgg ccgccttgat gcgcggcaat tcagcacggg cgccctacag acacgcgggg
    10201 atttcgtcag gtttctccgc cagacctgtg gcgagcaaac ctttcacgcg ccggagactc
    10261 cacgggaacg ccgcggcgtg cgatgcctgg agctgagaag cgtccggggt ctgcgtttcc
    10321 gctacgtgtt cgcaggcggt ttgaatgaag gcatcgtgcc cgggcagccc ggcgtcaacg
    10381 ccgtgtattc cgaagatgac gtgcgagagc tgcgcgatgc cgggatccct ctggagtccc
    10441 gtgaagtgcg cacagagcgg gaactggccg cgtttcatca cgtgcttgaa gcggctcaag
    10501 accggatctg gcttttctgg cgcacggcca cgccggatgg aaaacctttg ctgcgcagcc
    10561 cgtttttaaa tgatcttctt cgcctgtttg cggcaaagcc ggtcgaacgg acgcccctgc
    10621 gggccagtca gtattatccc gcgctttctc acgccgcttc ccaccgcgac gcaatcaaca
    10681 acctgttcgc cactggcaca cgtctgcccg aagcgcttgc agctgagttc gcggcagttg
    10741 tgcgcggagc ggatatcgag aagcgccgct acagcctcgc ggtcttcgat gcgtttgacg
    10801 gggtgctctg cgaggaacga aaccaggcgg agacagctga gcggtttggc gaggatcacg
    10861 ttttcagcgc gacggaattg gagtattacc gccagtgtcc gttccgtttt tttgcattgc
    10921 ggctcctgcg cttgatgccg gccgaacgtc cggtcacggc gtttgatcgc ggcctcctgg
    10981 gcatcgtcgt gcatcaggtg cttgagaaat ttcaccagca ctatgcgggc aaaccggtaa
    11041 gcgagattcc ggtgcgcgag gctgaggagg ccatgcgcgg ggtcgtcgat tcagtctttg
    11101 acgaaaatag cgcggccatg atcacagtgc ctgatgggat gctcatggcg gaacgcatgg
    11161 cagttcatga gcggctccaa cgctacctga ccatcgcgcg ccgggaagaa gtttcgtggc
    11221 gtccgacgga gttcgaagcg gttttcgggg ggaccaggga aaaggaatac ccgcccctgg
    11281 cgattttaac agaggccggc atactccgga tgcgcggccg catcgatcgg attgatcaag
    11341 gcggagcagg gtgccgtgtg gtcgactaca aaaccggcgg gcttccggcc ccagcacaag
    11401 taaaggaagg tatttcgctg cagctcgtgg cctatgcgct tgccctcgag aacgtgatcg
    11461 cgccggggac ggtctgcgag gaaggggtct tgctcaaggt aggtacgacc gaccggctgg
    11521 cggtgcggga gtccggccgc ggcacgctgt gggaggaagc aaagaaaggc tttttcgagg
    11581 gcgtatccgc ggcgcttaga gggattcgcg gcgcctcgtt tccaccggtg ccccagccgg
    11641 gcgtgtgcgc ttcttgcgat gcacggcgga tgtgccgcat cgatatgtcg cgggtcgagc
    11701 gaaaacaggg gggcgcgcca tgaaatggac cgacgcccag gccgaagcga ttcagacgac
    11761 aggcgaggac ctctgcgtga cggcctcggc aggctccggc aagactgcgg tgctggtcga
    11821 acgcgtgctg cacctggtga tcgaatgcaa cgttcctctt gaacgcattg tcgccatcac
    11881 gtttaccgag aaggccgctg cggagatgaa ggaccggctt cgcagcgagt gccgggcgcg
    11941 ggagcagagc gggtctaagg aagggcttga ccgctggcgc aacctggcgc gccaggtgga
    12001 gtcggcgcac atgagcacca tccacgcatt ctgtgcgagg ctgctgcgcc agtacgccct
    12061 ggaataccgc atcacgccgc ctctcgaccc cgattttgct gttgtggacg atgccgagag
    12121 ccgcttgctg cgcactgaag tggtcgatac ggccatcgag gagctcctcg aacggccgca
    12181 cgatgccacc atgcgtctgg cgacccacta cggagtgtac cagctcaagg aaatgctgga
    12241 gaggtttttg cgcaatcccg tgacggcatc ccggctactg tccaacgaac agttttcctc
    12301 gcccgaacgc ctcctgaaat tctggcgcgt gcgcgtggag gaaacctgcc gggcgcgctt
    12361 gctcaatctg agcaaatcgg ccgtctttta caactatctg cggaagttca aggaatttga
    12421 cgggcgctgc ggcatcccca cggatggccg ggaacagttt cgccgcacgt gcatccaact
    12481 catggaagaa gtgggccagc ttcgtgatcc cctgctgatc gagcagctga taatggcctg
    12541 catcgaagag agaataccca ccaccaggaa agccaattgg ccctcggaag aagtctttga
    12601 ggaacttaaa aagacccagg aggcctttaa aggctatttg cgggaatttt taatgccgga
    12661 ccatgacccg gacgtcgaac agcggagcgc cgaactgacg cgcgatgcag tcgcgtgttt
    12721 ccgggcggtt tttgagtact accgggaggc caagacctcc agaaacgtgc tggatttcac
    12781 cgatctcatt ctgttgacgc tcgatatgct ccagggccag ccggagctgc gcgagcgtat
    12841 cgcccggggc atcgaccacc tgctcattga tgaattccag gataccgact tcgaacagta
    12901 tgaaattgcc cgtctgctca ggtacacgcc ggctcccccg gaactgttca ttgtgggcga
    12961 tgcgaaacaa tcgatctacc ggtttcgggg cgccgaggtt gaagtctttg aggccgcgaa
    13021 acaagaaagc cgaaccatcg gtttgcaccg gaatttccgc acggttcccg aaattgtctc
    13081 gtttattaat gatattttcg atcgcagcgg catccttgaa gcggtggaat cgccgtttat
    13141 gcccgccgac gcccaccggg ctgccaccgg cgaatgccgg gtccatgtgc tgattcccga
    13201 cgctcctgag gacaaagagt tgagcgcgca aaggcgcatg cgcgaggcgg caatgattgg
    13261 ggattggatt gccgctgcgc attcgggcca actggatgtc caggtgcagg ccaagggcag
    13321 cgatacgacg cgcaaggcgg cattcggcga catggcgttg ctgttccgcg ccctcagcga
    13381 catccgcatc tacgagcgcg ccctggcgga acgccatatc ccgttccatg tcatcgccgg
    13441 aaaaggattt tacgaacggc aggaggtcct ggacctgcgc aatcttcttg aggcggtcgt
    13501 cgatccctgg cacgaacctg ctctgctggg attcctgcgg agtcccatgg ccggattgga
    13561 cgacgattcc attcttgcga tctgcgccgg cccgggcccg gtggccgcgc tccttgaaga
    13621 cggtactcca cccgatttca ggcagaccgc tggactcgat cacgcgcgcc ggatgttccg
    13681 ggatctgcgc gcggaacgtg agaggccttt accggatttt gtgcgttatg tgcttgaacg
    13741 gacgggatac gaggccatgg tggcgggcct tttcctgggc gaccagcggg cctgcaatgt
    13801 tcgcaaagtg atgcagctcg ccgagacgtt tgtgaagaca cgccggcccc ggctcagcgc
    13861 ttttgtgcgc tatctggacg atgtggcgtc tgcggagatc gatgaaggcg aggcaagcct
    13921 gcaggccgaa gggtccgacg aagtcacgct catgagcatc cacaaatcga agggtctgga
    13981 gtttccgatc gtgattcttc ccgacctgtc gcgcggttcg catcaaggcg ggaacgttgg
    14041 ccgcgtcgtg tttcaccggg acttcggaat tgtcgcgcgg ccgtacaacc ccgcatcgga
    14101 gcggcaaaca gagccgcaga tctacactgt gatgaagcgg gacgaagagg ccaaggagga
    14161 ggcggaacgc gcgcgggtgt tgtacgtggc catgacgcgg gcgcgggact ggcttgtcct
    14221 gagtggtact gtcgcttcgg gcaaaagccc cagacccgct ccggacagcc tgctggagcc
    14281 gatcgccagt gagctggatg tactgaaccg tggcgacggg gcgttgcttt ccggtgaggg
    14341 ctggaccgcg ctcgtccgcc gttccacgac gcccgcagcc atcgacgccc cagcccctga
    14401 gccggcgct
//
LOCUS       DAOPBQ010000022        14408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4239_length_14408_cov_3.803943, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000022 DAOPBQ010000000
VERSION     DAOPBQ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 14408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14408
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4239_length_14408_cov_3.803943"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..257
                     /locus_tag="PKW60_01600"
                     /pseudo
     CDS             39..257
                     /locus_tag="PKW60_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010384035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(348..2804)
                     /locus_tag="PKW60_01605"
     CDS             complement(348..2804)
                     /locus_tag="PKW60_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98155.1"
                     /translation="MMLKSVWLGMLICGVAVLSFGASPVSMTVTEVLREPLPSEDLSC
                     IESDAAIVVESPACRYTVDRASGAIAGLETKRDGLVQLQQAAELWFDDTPLSGCGGGK
                     TDIAVQGKDKIVLNTRGALRDGVPYTLETTFYNDGVVVCALTLNPATDLSFTRGIRFE
                     VSAAGQFSHYLHKRRDSEGVDSFQGALPEAGQTVELTTLTSCLEVFSSKAALAIFTDR
                     GGTFRQPADAPLASLGVGEKLPGSRMPVLLTQHIVRVAPGGDPFVVRAGESFTFRTGL
                     AVAPNRLPHPRWRDLRMFIWIGDDKHPYPADEEIRAAARLGFTLFQMHRLGTPGEPRP
                     PAGEFDRVLKTVHDAGMLFIWTANADLMYANALGVQERIAAEQWPLWQGFNYGGRYKA
                     AMDAYCDLIATCLASPNGLADYRMETIGRMLENYPVDGMYIDDNLPYANCTLWEEHGH
                     PEPVYDCLIELHEVNWRRRQALLAKIPHAVLIDHSSHGFILPAISAFDCHLFGEGYSF
                     PSVEAYWAQYGSYHNQPAQGCLWAGDTEAARCGTELAYAYDLLTGGGQYSYLDWRLWP
                     DKFPYASGVNPDEVLFVEFFNAAQHYFGLYESDLYLFPDSRDWFAATAPGTYATLYHN
                     RTWNDALVVVANPNGAPASTAVAFTERTLPPFEADRPLAVYDVLEHTIKAGRGREALA
                     HLTDISLQPNQTRLFYVREAPENTAYHQWGGKRISEQWDPVNGTLSLVLHGPAGREDC
                     VIIATGGQKTARVTVNGTPAAFFHDPARGLVWGNVVFGPAPISIVATRASEDTAQLPE
                     QALPDNALSACYASREDPVP"
     gene            complement(2847..4559)
                     /locus_tag="PKW60_01610"
     CDS             complement(2847..4559)
                     /locus_tag="PKW60_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98156.1"
                     /translation="MMLQRSMCIRAAVGVTAVVLAALTAAAGEEKQNYSALPWALRDH
                     YVFSFDEDRAQFLYATDNGEWTIHFEGFGEFIDKARASITLGDDTVVQLADFDRGAAD
                     RERADTPMGPCSDFRSEFPERNGLKVLHRVSVQNDYPFLVVRMEVTNTGEAPVEIKKL
                     SPVIFGPGCMKDASGKMESAIRRLDMRAGYATFDRTAKSSLAFFRDTTNNRTLALGVL
                     PLHIARSGINLEPFEGAWQGEIVSVFDPPIRLEPGASLAADPVFLTFSVPKPEDVETY
                     FTWIRSKLPCPAALADAPRFWCTVEEDASAEALYAEAAKWAAAGLKHALVPATWESRP
                     GSLEGARPRYPKSMQSVASSLSAAGMTPGITVDPLATLDGGEAFAASIAGHTWLNLSH
                     PDGRNQAVANMRKIAGWGFQFFVIQKSTVPDEVLRAFNMTRTQADALAHEVMTEAAGS
                     LPVFPSAATTLKDGLDDWLEASGCTSRLWEYRIAAAPVRFDVAGVRELSDEMSTVLAF
                     FGGPIELIGTPHRNLASQIAGIKHRTGQPLDIMQRAPRTWLVPSDESGSGEKTQMTFS
                     APAS"
     gene            complement(4732..5850)
                     /locus_tag="PKW60_01615"
     CDS             complement(4732..5850)
                     /locus_tag="PKW60_01615"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HPJ98157.1"
                     /translation="MISSIAVLVAAATLITGQTPAAVSAGQEITLARGRLFAPAWVNG
                     VGPYPFLIDTAAPYVALDAPIAGALGLPDAGKPEEADGFSAHRIRIQALEVAGRPALE
                     CSAYATDLAPMEALYGLRVAGILGLPGLPERFLLDCEGGRLARYPLQMGPEWIAVPIS
                     TGSDGVMRVPVTVNGEHAVEAELDTVFSGTLALPRNLLEQWDLFTEHTPRLEVADGAG
                     SRGAIQIRLPKCTVGPLEVETPLCEVLENGAAPQLGMRFWSRFMPALDRAAGTLHVLP
                     LVPLPWHDPPVHSAGVTPAGQRGGLWRLFIAEGSPAQRAGLLTGDLLVSINGMDMAEQ
                     SFDYVHRALTGQPATALEIAVFRSGEVFAVLVAVDQAL"
     gene            complement(5954..6751)
                     /gene="dapB"
                     /locus_tag="PKW60_01620"
     CDS             complement(5954..6751)
                     /gene="dapB"
                     /locus_tag="PKW60_01620"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPJ98158.1"
                     /translation="MKICVAGACGRMGRRILELAAATDDIAIGGAFDVAAFAGTELIA
                     GAESGRPQRVMVSADPAAELAESDCLIDFTAADACVGNVRLAADAGKPCVLGTTGLSE
                     AQVAVVNDCATRIPVVYAPNMSVGVNLLFKLTGEVAALLGLDYNVEICEIHHNQKKDS
                     PSGTAVRLGECAAQALGLDYARDTAHGREGLVGARPRNQIGMHALRGGDVVGEHTVSF
                     IGQGERIELTHKAHNRDNFARGALVAARFTMTAAPGLYDMQDVLGLK"
     gene            complement(6781..7653)
                     /gene="dapA"
                     /locus_tag="PKW60_01625"
     CDS             complement(6781..7653)
                     /gene="dapA"
                     /locus_tag="PKW60_01625"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPJ98159.1"
                     /translation="MFRGSIVALVTPFKEDFDIDFEAYGRLIDWHLESGTDGLVPCGC
                     TGEAATLTHDEQIQCIKYTVERVSGRVPVIAGTGSNSTREALSLTRKAKELGADGALL
                     ITPYYNKPTPAGQMAHYTTIARQVDIPIVLYNVPGRTGTKMLPATIADIHSAADNIVA
                     VKEACGSVDQVSEIRSLSSITVLSGDDSLTLPMMSVGATGVISVAANVMPAKVAAMCA
                     AFDQGDLAEAQRIHYDLLPLVKGLFLETNPMPVKAALHRMGKINNVLRLPLVPMQPVL
                     FEKLERILGQVGAI"
     gene            7984..8115
                     /locus_tag="PKW60_01630"
     CDS             7984..8115
                     /locus_tag="PKW60_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98160.1"
                     /translation="MPVLSQLRELVSDRLAAQWRFPEAALALGRQLIADIEFGAFLA"
     gene            complement(8131..8970)
                     /locus_tag="PKW60_01635"
     CDS             complement(8131..8970)
                     /locus_tag="PKW60_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98161.1"
                     /translation="MPRKSLVILIAGLACAGAWAQLFPEERDALDALLSQVERSLTTQ
                     SPPLWSLFSPLGSLAVMANENGGWTTARRDELRRNAGTAAAFTLPLGARATFRVYETA
                     RELMMGQAIFESPEKRHWLATFATLPDGPGGACRLTALCIFPSDRLSPPEMSQDAVNV
                     VKAWERVVREGQMLELRPHLYPDPFLAILGKGGMELFLYVDPERYITMARYAVEVGRP
                     ETSEMSRLHACSNGAIATVTGTWKAPNVVFGLGDYRLTAILLKTDGDWKVAGLFMGTQ
                     TQY"
     gene            complement(8982..11114)
                     /locus_tag="PKW60_01640"
     CDS             complement(8982..11114)
                     /locus_tag="PKW60_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98162.1"
                     /translation="MPARVLPFLSLFMLVAAFGAAQTQPGVSSNGHVFNLERFGAGVP
                     GGWLLDGSDYTWEADTGPGRIGPGAGRLLFGDHGRIEITSPARHLESGRAYTLLLWFR
                     SEPADAALSVSLHDNETRQTLAFEQAATATREWQQLRLTHVLPKAGKDCYYLRLSANG
                     ANTTLWLDGLWLGATEAEPPADWQPEWHAAAVAVEPDAPWGVVAGTGPVRYRIRVAGA
                     TAPGAQLQTRIVSTSGETASQPPIPLDTTGSWTGTAEITGDIANRFGMFRVEATVVAP
                     SGTALSPMTETLLARAPEPFPAPFRDSYFGIHAKLIAPDLEAMARLGYKWLRLHDASG
                     ITKWGLIEPEPGRWLWYDDEVALAREKGFFILGLLDSAPPWDSGAEEEGYFSIYSAPK
                     NLENWRNYVRRVVAHYKGAIDDWEVWNEPWNPIGFFKGGNPQLYTELLKIAFTETRAV
                     HPEAVIVGIDTWPPYWDAMTLAAGAYDYYDVLSFHRYDPGMHGYPNDPIDRVAKRLRT
                     AQENYGEPKPLLFSEGGPDVTVFHGSFFSFAEPALTGDWSWCADQYARMFLTTIASGT
                     QRFFAYSVHNQPHHGESTHMMVEPGPLLRPLHLTAAALAHFVDGAHYVERLQPAHDIS
                     AHVFAQPHDRPYAAGGCTVVVLHANGLDPEPLPRPLPADTASCDRWGNPCALPTEALR
                     SPVYLVAAGSAASALTSALAPAEPDTQP"
     gene            complement(11230..11973)
                     /locus_tag="PKW60_01645"
     CDS             complement(11230..11973)
                     /locus_tag="PKW60_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPJ98163.1"
                     /translation="MAAPTRKERELRKREELFLQVARRLLLERGFHGLTMDRIAEETE
                     YSKGTIYLHFGCKEELLLELGKRSRRQRLDFITRGVAFEGRPRERMVAVGVAVEIHAR
                     LFPDDVRIWEIMNAESIIEKIPAERLPDLKASDIAANDILIGLVHEAIACGDLVLRPG
                     DSPQEITFGLWAITDGRFAAILGNAPLEHLNLRDPYAAIFKNCHVLGDGYGWRPLSTE
                     WDYEATTQRIRREVFPEESRVAFGGGEAA"
     gene            complement(12070..13452)
                     /locus_tag="PKW60_01650"
     CDS             complement(12070..13452)
                     /locus_tag="PKW60_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98164.1"
                     /translation="MTSLLLFEIALSFLGYTGPLSSVLAGEHLAAPEHATLPVQPTVT
                     VVHEETVYTHTPANNGSGPFWSSGCTILARVGDTVMLSEMETGHDVPPLCNTRWRLHS
                     RTDAGWSLFAEAEGYRQREPTLLATTGSDVFYLNVNDSTEPPGTKYGPCTPYLLKFHL
                     DNPSAPEALRPVWEGEPTFTDHSYRGYAADRGAGELLMLNIDAKTSEENWCHLKGDGE
                     VLGNGKISFPIRACYPQVALKDHAAYVLAISDIREPVKEWEDYKFEQTQNTWDYVFRI
                     LYFTWTPNIGSQAFFPPIEIANVDATAGHISGQDLHIAPNGDAYILYTQREVSSALMR
                     DKFFPDLSIVNSLHLALVREGQIIDRKTIIEGTDTSQPGSARFHIAANGDCYAFIYAT
                     IDNAPGNYLVPLNIPQDAPPLIPVPLRQPLSTFLLASPRAGNAPSNTIDLAGAGPAGA
                     IVYAQITLEP"
     gene            complement(13559..>14408)
                     /locus_tag="PKW60_01655"
     CDS             complement(13559..>14408)
                     /locus_tag="PKW60_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ98165.1"
                     /translation="MYLLLRAGHAAEARRAIPPNDAPIMGTLFRALARKLAGEPYLPL
                     LESLDEIDGDIDAESRDYLQEILFLYGPGPTGAFLLGLMRDIDAEGLRSQRFAILQAR
                     ILMAQERYAEAAPLLLAALEESGPGKWIHYYLAIIYDELKDPANTETHIEACLQLDPD
                     DPDVLNFLGYFFADHGRNLDRAETLIKRALELSPENGFYLDSLGWVYYRQGKPDLAIE
                     YIRRSILRLERDDAVVRDHLGDAYFLKGEVEKAVEEWERAHRLDPELEGVAEKLNQHR
                     AQPSQR"
BASE COUNT         2612 a         4398 c         4719 g         2679 t
ORIGIN      
        1 cagcgggata aaaacacaga ctcgtatcgg gtcgccacgg tcacggcggc ggcgactaca
       61 tcatgatgta ccgcctgatc aaatgcctgc agacgggcgt tgaacccgat tcggacgtct
      121 acgatgccgc cgcgtggagc gtgatcgcgc cgctcagcga gatgtccgtc gccgacaaga
      181 gccgtccggt cgatttcccc gatttcacgc gcggcaaatg gcgtgaacga cagcccctgg
      241 gcatcgtgga ggcgtaaacc gggcgcgtcg cggtcaaaac accggaattg agcttggacg
      301 agcgggcggg tgcgcaggca cccgcccgtt ctcatatgcg gggccggtta cgggaccgga
      361 tcttcccgtg acgcatagca ggcgctgagt gcgttgtctg gaagtgcctg ttcaggcagc
      421 tgcgcggtgt cttcagaggc gcgggtcgcg acaatactga tcggtgcggg tccgaatacg
      481 acatttcccc agaccagtcc gcgcgcaggg tcatggaaga aagccgcggg cgttccattc
      541 acggtcacac gggctgtctt ctggccgccg gtggcgatga tcacgcaatc ttcccggccg
      601 gccgggccgt gcagcacgag agacagggtc ccgttcacgg gatcccactg ttcagagatt
      661 cgtttgccgc cccactggtg gtatgccgtg ttttcagggg cctcgcgcac gtagaacagc
      721 cgggtctggt tgggttgcaa cgaaatgtcc gtgagatgcg cgagggcttc ccggccccgc
      781 ccggccttta ttgtgtgttc gagcacgtca tagaccgcaa gaggccggtc cgcctcaaat
      841 ggcggcaacg tccgttcagt gaaggccaca gccgtgctgg ccggtgcacc gttcggattc
      901 gcgacgacga ccagggcgtc attccacgtg cggttgtgat acagcgtggc ataggtgccg
      961 ggcgcggtgg cggcgaacca gtcgcgcgag tcggggaaga gatagaggtc ggactcgtac
     1021 aggccgaaat agtgctgggc ggcgttaaaa aactctacaa acaggacttc gtcggggttg
     1081 acccctgagg cataggggaa cttgtccggc cagagccgcc aatcgaggta gctgtattgc
     1141 ccgccccccg tgagaagatc gtacgcgtaa gcgagctcgg tgccacagcg cgccgcttcc
     1201 gtatcgcccg cccagaggca accctgagcc ggctgattgt ggtacgaccc atactgcgcc
     1261 caatacgctt cgaccgatgg aaaagaataa ccttcaccga agaggtggca atcaaacgcg
     1321 gatatggcgg gcaggatgaa cccatggctc gaatggtcga tcagcacagc gtgcgggatt
     1381 ttggccaaca gggcctggcg ccgccgccag ttcacctcgt gcagctcgat caggcaatca
     1441 taaaccggct cggggtggcc gtgctcttcc cagagggtgc agttggcgta gggcaggtta
     1501 tcgtcgatat acatgccgtc aacgggatag ttctcgagca tccgcccaat ggtctccatg
     1561 cggtaatcgg caagcccgtt gggcgaggcg aggcaggtgg cgattagatc gcaataggcg
     1621 tccatggcgg ctttgtagcg tccgccgtag ttgaaaccct gccagagcgg ccattgctcc
     1681 gcggcgatgc gttcctggac cccgagcgcg ttggcataca tgaggtctgc gttggccgtc
     1741 cagatgaaca gcatgccggc atcgtggacg gttttcagca cgcggtcgaa ttctccagcg
     1801 ggcggcctcg gttcgcccgg agtgcccagg cgatgcatct ggaacagcgt gaatccaagc
     1861 cgcgcggctg cgcgaatctc ctcatcggca gggtaggggt gtttgtcgtc gccaatccag
     1921 atgaacatcc gcaggtcgcg ccatcgggga tgaggcaggc gattcggggc aacagccagg
     1981 ccggtgcgga acgtgaatga ttcgcccgcc cgtacgacga aagggtctcc gcccggcgct
     2041 acgcgcacga tatgctgtgt gagcagtacc ggcatgcgac tacccgggag cttctcgcca
     2101 accccgaggg aggcgagcgg ggcgtcggcc ggttgccgga aggttccacc ccggtccgta
     2161 aaaatggcca gggcggcttt ggaactgaag acctcgaggc aagacgtgag tgtggtcagc
     2221 tcgacagtct gcccggcttc gggaagcgcg ccctggaacg agtcgacgcc ttctgaatcc
     2281 cggcgcttgt ggaggtagtg actgaactgc cctgccgcgc tgacttcgaa tcggattcct
     2341 ctggtgaacg aaagatccgt cgcgggattg agcgtcagcg cgcagaccac gacaccgtcg
     2401 ttgtagaacg tcgtttcgag cgtatacggc acgccgtctc gcagggcgcc gcgcgtgttc
     2461 aaaacgatct tgtcttttcc ctggacggcg atgtctgttt tgccgccgcc gcatcccgac
     2521 aggggcgtat catcgaacca cagttcggcg gcttgctgga gttggactag cccgtcccgc
     2581 ttggtttcca gtccggcaat ggcacctgat gccctgtcca cggtgtagcg gcaggccggg
     2641 ctttcgacga caatggccgc gtcgctctcg atacagctca aatcctcgga cggcagcggt
     2701 tcacgcagca cctccgtcac cgtcatgctc accggcgagg caccgaaact caggacggca
     2761 acgccacaaa tcaacatgcc aagccacact gatttcagca tcatgcgaga tctcccgtgg
     2821 tcggcgcgga ttcctgtcgc gcggtgttat gaagccggcg ccgaaaaggt catttgcgtt
     2881 ttttcaccgc tgccggactc atcgctcggc acgagccagg tacgcggcgc gcgctgcatg
     2941 atgtcgagcg gttgccccgt gcggtgtttg atacccgcga tttgagacgc gaggttgcgg
     3001 tgcggtgtgc cgatcagctc gatgggtccg ccgaagaagg ccaggacagt gctcatctcg
     3061 tcgctcagtt cacgcacgcc ggccacatca aaacgcacgg gggccgcggc gatccggtat
     3121 tcccaaagcc ggctggtgca tccggacgcc tccagccaat cgtccaatcc atccttgagc
     3181 gttgtcgcgg cggagggaaa cacggggagg ctgcccgcgg cctcggtcat gacctcgtgg
     3241 gcgagtgcat cggcctgggt gcgggtcatg ttgaaggcgc gcagcacctc gtcggggact
     3301 gtggatttct gaattacgaa aaactggaac ccccatccgg cgattttgcg catgttggcg
     3361 acggcctggt ttcggccgtc gggatgcgaa aggttgagcc acgtgtggcc ggctatcgat
     3421 gccgcaaacg cctcgccgcc gtcgagggta gcgagcgggt cgaccgtaat gccgggcgtc
     3481 atcccggctg cgctgagcga ggaggctacg ctctgcatcg atttgggata ccggggccgg
     3541 gctccttcca gggagcctgg gcgcgattcc caggtggcgg gcaccagagc atgtttgagc
     3601 cccgcggcgg cccatttcgc ggcttcagca tacagggcct cggcgctggc atcctcttca
     3661 acggtgcacc agaaccgggg ggcgtctgct aatgcggcgg ggcaggggag tttgctgcgg
     3721 atccacgtga aataggtctc cacatcttcc ggtttgggca cggagaaggt gagaaacaca
     3781 gggtcggcgg ccagtgaagc accgggttca agccggatgg gcggatcaaa taccgacacg
     3841 atctcgccct gccatgcgcc ttcgaacggt tcgaggttga tgccggagcg ggcgatatgc
     3901 agcggcagaa cgcccagggc gagcgtgcgg ttgttcgtgg tgtcgcggaa aaacgcgagc
     3961 gacgatttgg ccgtccgatc aaacgtggcg tagcccgccc gcatgtcgag ccgccggatg
     4021 gcgctttcca tcttgccgct ggcgtctttc atgcatccgg gcccgaagat caccgggctc
     4081 aacttcttga tctcgacagg tgcttcgccg gtgttggtga cctccatgcg cacgaccagg
     4141 aagggatagt cgttctgcac ggacacccgg tgaagcactt tcaggccgtt acgctccgga
     4201 aattccgacc ggaaatcgct gcacggcccc atcggggtat ccgcacgctc ccggtctgcg
     4261 gcgcctctgt cgaaatcagc cagctggacc accgtgtcgt cgcccagcgt gatcgacgcg
     4321 cgggccttgt caatgaattc accaaaacct tcgaagtgaa tggtccactc cccattatcc
     4381 gtggcgtaca gaaactgcgc gcgatcctcg tcaaacgaaa acacataatg gtcgcgcagg
     4441 gcccacggga gagcggagta attctgtttc tcttcacctg cggccgccgt caaggcagcc
     4501 aggacaactg ccgtcacgcc aacggccgcc cggatgcaca tcgaacgctg gagcatcatg
     4561 aaccctttct agaatgttgc aggcgcatga ggtctgtcat gcgcgtgggt tttccacatt
     4621 ttcaaggcgg gattatacac gataccggtc cacaaccggg tatgctgtga tctgaatgcg
     4681 gaaagcgccc tggaacggcc cgggtgcgtg ctggcctccg cgcttgtccg gttaaagcgc
     4741 ttgatccacc gctaccagaa cagcgaaaac ctcaccgctc cgaaacacag cgatttccag
     4801 ggcggttgcg ggctgccctg tcaaagcgcg gtgcacataa tcaaacgatt gctccgccat
     4861 gtccattcca ttgatggata ccagcagatc gccggtgagc agccccgccc gttgcgcggg
     4921 agagccctcg gcgatgaaca ggcgccacag gccgcccctc tgaccagcgg gggtgacgcc
     4981 ggcgctgtgc acaggaggat cgtgccacgg cagcggcaca agcggcagca cgtggagcgt
     5041 tcccgcagcg cgatcgagcg ccggcatgaa acgcgaccag aatcgcatgc ccagctgcgg
     5101 cgccgcgccg ttttcaagca cctcgcagag gggcgtctcg acctcaagcg gcccgaccgt
     5161 gcacttcggc agacggatct ggatggcgcc ccggctcccg gccccgtccg ctacttccag
     5221 gcggggggtg tgttcggtga agaggtccca ctgctccagg agattccgcg gcagtgcgag
     5281 cgttccgctg aataccgtgt cgagttcggc ctcgacggca tgttctccgt tcactgtgac
     5341 gggcacgcgc atgacgccgt cggatcccgt cgagatgggc acggcaatcc attccgggcc
     5401 catctggagc gggtaccggg caaggcggcc gccctcgcaa tccaggagaa aacgttccgg
     5461 aagtcccggc agcccgagaa tgcccgcgac ccgcaggcca tagagtgctt ccatgggggc
     5521 caggtcggtc gcgtaggcgc tgcactcgag cgcggggcgg cccgcgacct cgagggcctg
     5581 gatccggatc cggtgcgcgg aaaacccgtc ggcctcctcc ggcttgcccg cgtcaggcag
     5641 gccgagcgcg ccggcgatgg gggcatcgag cgcaacatag ggcgctgcgg tgtcaatgag
     5701 aaacggatag gggcctacac cgttcaccca ggccggcgcg aagagccgac ctcgggcaag
     5761 cgtgatctcc tgcccggcag acacggcggc gggcgtctgg cccgtgatga gcgtggccgc
     5821 tgccaccagg acggcaattg aagagatcat gggatcttct ccgcgagcat atccgccgga
     5881 aagcgagtcg acaccatcgc tttcgggacc gggccgcgct gtccttcaga caggcctccg
     5941 gcgagacggc gggtcatttc aggccgagga cgtcctgcat atcgtagagg ccgggcgcgg
     6001 ccgtcatggt gaagcgggcc gccacgaggg cgccgcgcgc gaaattgtcg cggttgtgcg
     6061 ctttgtgggt gagctcaatg cgctcgccct gcccaatgaa gctgacggtg tgttcgccga
     6121 caacatcgcc gccgcgcagg gcatgcatgc cgatctggtt gcggggccgc gcgcccacga
     6181 gtccttcgcg gccatgggcg gtatcgcggg cgtaatcgag cccgagcgcc tgagcggcgc
     6241 attcgcccag gcgcaccgcc gtgccgctgg ggctgtcttt cttctggttg tgatggattt
     6301 cgcagatctc gacgttgtaa tcaagtccca gcagggcggc cacctcgccc gtcagcttga
     6361 acaggaggtt gacgcccacg ctcatattgg gtgcgtatac gacaggaatg cgcgtggcac
     6421 agtcgttcac cacggcgacc tgggcctcgc tcaatccggt cgtgcccagc acgcacggct
     6481 taccggcgtc cgcggccagg cggacgttgc caacgcacgc gtccgccgcg gtaaaatcga
     6541 tcagacagtc agattcagcc agttcagcgg cggggtcggc cgataccatg acccgctggg
     6601 ggcgtcccga ttcggcgccg gcgatgagtt cggtcccggc gaaggcggct acgtcaaacg
     6661 cgccgccgat ggcgatatcg tccgtggccg cggccagttc caggatgcgc cggcccatgc
     6721 ggccgcaggc gcctgccacg cagattttca tgatctaccc tttcccggag aagcaggccg
     6781 ctaaatcgcg cccacctgcc ccagaattcg ttccagtttt tcgaagagta ccggctgcat
     6841 cggcacgaga ggaagccgca gcacattgtt gatcttgccc atgcggtgga gggccgcttt
     6901 cacgggcatg gggttggttt ccaggaagag ccccttgacc agcggcagaa ggtcgtaatg
     6961 gattcgttgc gcttcggcca aatcgccctg atcgaacgcg gcgcacatcg ccgccacttt
     7021 cgcgggcatg acattcgcgg ccacggagat cacgccggtc gcgccgacgc tcatcatcgg
     7081 cagcgtcagg ctgtcgtcgc ccgaaagcac ggtgatgctg ctcagactgc ggatttccga
     7141 gacctggtcc acgctgccgc aggcttcctt caccgccaca atgttgtctg cggcgctgtg
     7201 gatgtcggcg atggtggccg gcagcatttt ggtgcccgtg cgccccggca cgttatagag
     7261 cacgatggga atgtcgacct gccgggcgat ggtggtgtag tgggccatct ggccggccgg
     7321 cgtgggcttg ttgtaatacg gcgtgatgag gagtgcgccg tccgcgccca gctctttcgc
     7381 cttacgggtc agcgacaggg cttccctcgt gctgttcgaa cccgtgcccg cgatcacggg
     7441 cacccgtccc gacacgcgct cgacggtata tttgatgcac tggatctgct cgtcgtgggt
     7501 cagcgtcgcc gcttcccccg tgcagccgca cggaaccagt ccatccgttc cgctttccag
     7561 atgccagtcg atgaggcggc cgtacgcctc gaaatcgatg tcaaaatctt ctttgaacgg
     7621 cgtcacaagc gccacgattg aaccacggaa catggagtat ctccttcgtg aatcgtcacc
     7681 cctgccttgc ctggattgtc cgcgttccgc gatacagaga agcccaggtt gaccgccatt
     7741 gtaaaacatt cgacgtgaga tacgccacac agcgaatcgc gccctctaag gtcagactgg
     7801 tgttgccatg taattttgcg tcctggcctg ctctgccgcc gcaagagccg cggttaatcc
     7861 catccactcc gtccaccctg tccacattct catcccctac tcttcttcaa caggtcgcag
     7921 cccggctgat tgccgaaaaa acagggcgcc cggtccgggt ggggagctgc gcgggccgag
     7981 gaaatgccgg tactgtccca actgcgcgaa ctggtgtccg accggcttgc cgcgcagtgg
     8041 cggtttccgg aagcagcgct ggcgctcggc agacagctca ttgcggatat cgaattcggc
     8101 gccttcctgg catgacatcg ccgcgtgtgc tcagtactgc gtctgtgtgc ccatgaacag
     8161 gccggcgacc ttccagtctc cgtcggtctt gagcaaaata gcggtgagcc ggtagtcacc
     8221 gaggccgaac accacgttgg gtgctttcca ggtgccggtg accgtggcga tggcgccatt
     8281 cgagcacgcg tggagcctgg acatttcgga ggtttcaggc cggcccacct ccacggcgta
     8341 gcgtgccatt gtgatgtaac gctcgggatc cacatacagg aacaattcca tgccgccttt
     8401 gcccaatatg gccagaaacg ggtcggggta gagatgcggg cgcagttcca gcatctggcc
     8461 ttcccggaca acccgttccc acgccttcac cacgttcacg gcgtcctgcg acatctccgg
     8521 aggggaaagc cggtcggagg gaaagatgca cagggcggtc aggcggcagg cgcccccggg
     8581 accgtcgggc agcgtcgcga acgtcgccag ccagtgcctc ttttcaggag attcgaaaat
     8641 ggcctgcccc atcatcagtt cgcgggcggt ctcgtagacg cggaaggtgg cccgggcgcc
     8701 caggggtaaa gtgaaggccg cggccgtgcc ggcgttacgc ctgagctcat cgcgccgggc
     8761 ggtagtccag ccgccgttct cattggccat cacggccagg gaccccaggg gcgaaaacag
     8821 cgaccacagc ggaggcgact gcgtggtaag ggagcgctca acctggctca ggagcgcatc
     8881 gagagcgtcg cgttcttccg ggaacagttg ggcccatgcg cccgcgcacg cgaggccggc
     8941 aatcaaaatg acgagactct ttcgcggcat ggcgtgtgct cctatggttg cgtgtccggt
     9001 tcagctggcg cgagggcgga cgtcaacgcg ctggcagcgc tgccggccgc caccagatag
     9061 accgggctgc gcagcgcttc cgtcgggagg gcgcacgggt tgccccagcg gtcacaggaa
     9121 gcggtatccg cgggcaatgg gcgcggcaaa ggctccggat ccaggccgtt tgcgtgcaac
     9181 acgaccacgg tacagcctcc cgctgcgtac ggccggtcat gaggctgagc aaacacgtgc
     9241 gcgctgatgt cgtgcgcggg ctgaagccgc tcaacgtaat gcgcgccgtc gacgaaatgc
     9301 gccagggcgg ccgcggtaag gtgaagcgga cgcagcagcg gtcccggttc caccatcatg
     9361 tgcgtcgatt ctccgtgatg cggttggtta tggacggaat acgcgaagaa tctctgggtt
     9421 ccggatgcga tcgtggtgag gaacatccgc gcgtactggt cggcgcacca cgaccagtcg
     9481 ccggtaagcg ccggttccgc gaaagagaaa aaagacccgt ggaagaccgt cacgtcgggc
     9541 ccgccttcgc tgaacaggag gggtttgggc tcgccgtagt tctcctgagc cgtgcgcagc
     9601 cgcttcgcta cgcggtcgat gggatcgttc ggataaccgt gcatgccggg atcgtaacgg
     9661 tgaaacgaca ggacgtcgta gtaatcgtag gctccggcgg cgagggtcat cgcgtcccaa
     9721 tagggaggcc aggtgtcgat gccgacgatc acggcttcag ggtgaaccgc ccgggtttcc
     9781 gtgaaggcga tcttgagcag ttctgtgtag agttgcggat tgcccccttt gaagaacccg
     9841 ataggattcc agggttcgtt ccagacctcc cagtcatcga ttgcaccctt gtaatgcgcg
     9901 accacacgcc gcacgtaatt gcgccagttt tccaggtttt tcggggcgct gtagatgctg
     9961 aaataaccct cttcctccgc gccggaatcc cagggcggcg cgctgtcaag caggccgagg
    10021 atgaagaacc ccttttcgcg ggccagcgcc acttcgtcgt cgtaccagag ccaacggccg
    10081 ggctcgggct cgatcagccc ccatttggtg atgccggacg cgtcgtggag ccgcagccac
    10141 ttgtaaccca gccgcgccat ggcctccaga tccggcgcga tcagcttcgc atggatcccg
    10201 aaatacgaat cacggaacgg cgcgggaaac ggttccggcg cccgggccag cagggtctcg
    10261 gtcatgggcg acagggccgt cccggaaggg gcgacgaccg tggcctcgac gcgaaacatc
    10321 ccaaaccggt ttgcgatatc tccggtgatc tcggccgtgc cggtccagga acccgtggta
    10381 tcgagcggga tggggggttg gctggcggtc tcgccggagg tggagacgat gcgtgtctgc
    10441 agctgcgctc ccggagcggt cgcgcctgcc acgcgtatcc ggtaacgcac cggtccggtc
    10501 cccgccacca cgccccacgg cgcatcgggt tcgacagcca ccgcggccgc gtgccattcc
    10561 ggctgccagt cggcaggggg ttctgcctct gttgcaccca gccagaggcc gtccagccag
    10621 agagtcgtgt tggcgccgtt tgctgataga cggaggtaat agcagtcttt tcctgccttc
    10681 gggagcacgt gggtgaggcg cagctgttgc cattcccgcg tggccgtagc cgcctgctca
    10741 aaggccaggg tttggcgggt ctcgttgtcg tgaagggaga ccgagagtgc tgcatccgcc
    10801 ggctcggaac ggaaccacag gaggagcgtg tacgcccggc cggattccag gtggcgtgct
    10861 ggcgaggtga tctcaatgcg tccgtggtcc ccaaaaagaa ggcgcccggc gccgggcccg
    10921 attcgaccgg gcccggtgtc agcctcccag gtgtagtcgc tgccgtcgag caaccatccg
    10981 ccagggacac ctgctccgaa gcgttcaaga ttgaacacat ggccgttgga cgagacgccg
    11041 ggttgcgtct gtgccgcgcc gaacgcagcc acaagcatga agagactcaa aaacggaaga
    11101 actcgtgccg gcatggcatt tatccaggtt gcggtttgtg tttgtcttgt ggcccgatgt
    11161 ctggtcaaca tataccggtc tgagggccat ccgaatgccc cgcaccgccg cgggtggccg
    11221 tcagcccggt caggctgctt ctccgccgcc aaaggccacg cgggactcct ccgggaacac
    11281 ttcacgccgg atgcgttggg tggtcgcctc gtaatcccat tccgtggaca agggtcgcca
    11341 cccgtatccg tcgccgagca catggcagtt tttgaatatc gctgcgtagg gatcccgaag
    11401 gttcaaatgt tccagcggcg cgttcccgag aatggcagca aacctgccgt ccgtgatggc
    11461 ccacagcccg aacgtgatct cctgaggaga gtcgccgggg cgcaagacca ggtcgccgca
    11521 cgcgatcgcc tcgtgcacca atccaatcaa tatgtcattt gcggcgatgt ccgaggcttt
    11581 caggtcgggc agccgctccg ccgggatctt ctcgatgatg gactccgcat tcatgatttc
    11641 ccagatgcgg acatcgtcgg gaaacagccg cgcatggatc tccactgcca cgccaaccgc
    11701 caccatgcgc tctcgcggac ggccttcaaa cgcgacgcca cgggtgatga agtcaaggcg
    11761 ctggcggcgc gaccgctttc cgagttcgag aagcaactcc tccttgcacc cgaaatgcag
    11821 gtagatcgtg cctttggaat actcggtttc ttcggcgatg cgatccatgg tgagaccatg
    11881 aaaaccgcgt tcgaggagta aacggcgcgc tacctggaga aagagctcct cgcgcttgcg
    11941 aagttcgcgt tctttgcgag ttggtgcggc catgactccg ccatggtatg acgcaccgtc
    12001 agaagttgtc aatgcgtcag cccggcggct caaaatgccg cagccggggg tgtcagccgg
    12061 gcgggccggt tacggttcca aagtgatctg ggcgtacacg atggcgcccg cggggccggc
    12121 gccggccagg tcaatggtgt ttgagggggc gttgcccgcg cgcgggctgg ccagaagaaa
    12181 cgtggacaac ggctggcgga gagggacggg gatgagcggg ggcgcatcct gtggaatatt
    12241 caaggggaca aggtagttgc ccggcgcgtt gtcgatggtc gcgtagatga aggcgtaaca
    12301 gtcgccgttc gcggcaatat ggaaacgcgc gctgccgggc tggctcgtgt ctgtcccctc
    12361 gatgatcgtt ttccggtcga tgatctggcc ctcgcgcaca agcgcgaggt gcagcgagtt
    12421 cacgatggac agatccggga agaacttgtc gcgcataagg gccgagctta cttcgcgctg
    12481 ggtatacagg atgtacgcgt cgccattggg ggcgatgtgg agatcctggc cggaaatgtg
    12541 gcctgcggta gcatcgacat tggcgatctc gatgggaggg aagaatgcct gcgagccgat
    12601 attgggcgtc caggtgaaat agaggatgcg aaagacgtaa tcccaggtgt tctgcgtctg
    12661 ctcaaacttg tagtcttccc attctttgac gggctcccgg atatcgctga tggcgagcac
    12721 gtaggcggca tggtctttca gcgccacctg gggatagcag gcccggatgg ggaacgagat
    12781 ttttccgttg cccagtacct cgccgtcgcc tttcagatga caccagtttt cctcactcgt
    12841 tttggcgtca atattgagca tgaggagctc cccggcgccg cgatctgctg cgtagccccg
    12901 gtacgaatgg tccgtaaacg tgggttcccc ttcccatacc ggccggagcg cttcgggggc
    12961 gctggggttg tcgaggtgga atttcagaag gtaaggcgtg caggggccgt atttggtgcc
    13021 tggcggttcc gtgctgtcgt tcacattaag atagaaaaca tcggaacccg tggttgccag
    13081 aagggtcggt tcgcgttggc gatagccctc ggcctcggca aacaggctcc atcccgcatc
    13141 cgtgcggctg tgcagccgcc agcgggtgtt gcagagcggg ggcacgtcat ggcccgtctc
    13201 catctccgag agcatcactg tgtcgccgac acgggccagg atggtgcagc cggaactcca
    13261 gaacgggccg gaaccgttgt ttgcgggggt atgcgtataa accgtctctt catgcacgac
    13321 cgtaactgtc ggctgcaccg gcagggtggc gtgttcagga gcggccagat gttcgccggc
    13381 gagtacgctg gacagcggtc cggtgtaacc caggaaagaa agtgcaattt cgaagagaag
    13441 cagcgaggtc acggagaatg ctcctttctg cggcggccat cgccgcccgt ttgtccggga
    13501 actggtccgg caaccggtac aaggccatgg tgtccggtat ggtgagattg ggttcagatt
    13561 atcgctgtga cggctgtgcg cgatgctgat tgagcttttc ggccacgcct tccagttccg
    13621 ggtccaggcg atgcgcccgt tcccattctt ccaccgcctt ctccacctct cctttcagga
    13681 aatacgcgtc gccgagatgg tcgcgaacca ccgcatcgtc acgttcgagc cgaaggatgg
    13741 agcgccggat gtattcgatc gctaaatcgg gttttccctg ccggtaatag acccaaccca
    13801 ggctgtcgag atagaaacca ttctcgggcg acagctcgag cgcccgcttg atcagcgttt
    13861 ccgcgcgatc gagattcctg ccgtggtccg cgaagaagta tcccagaaag ttcagcacat
    13921 ccggatcgtc gggatcaagt tgcaggcagg cttcaatgtg cgtttccgtg ttggccgggt
    13981 ctttcagttc atcgtagatg atggccaggt aataatggat ccatttgccc gggccggact
    14041 cttcgagggc ggccagcagc agaggcgccg cttccgcgta tcgttcctgg gccatcagaa
    14101 ttcgtgcctg gagaattgca aaccgctgcg aacggaggcc ttcggcgtcg atatcgcgca
    14161 tgagcccgag caggaaggcg ccggttggcc cggggccgta cagaaagagg atttcctgga
    14221 ggtaatcgcg gctttcggcg tcgatgtcgc cgtcgatttc gtccagggat tccaggagcg
    14281 gcagataggg ttccccggcg agcttgcggg ccaatgcgcg gaacagggtg cccatgatgg
    14341 gcgcgtcgtt tggcgggatg gcccgtctgg cttcggccgc gtggcccgcg cgcaaaagca
    14401 ggtacatg
//
LOCUS       DAOPBQ010000023        13851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4436_length_13851_cov_4.093288, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000023 DAOPBQ010000000
VERSION     DAOPBQ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 13851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13851
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4436_length_13851_cov_4.093288"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKW60_01660"
     CDS             <1..478
                     /locus_tag="PKW60_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98166.1"
                     /translation="TADVLLDAGAAALNIIPDRNWNVKSPDERHAKVEKLHEIVAIAE
                     ERGLPVLAGTEMNAHGQKFVDEFGVPEMAPLRETFMNGALILYAHTLLERAGGMGYLS
                     DWAKRHFASPRDRNAYFVELGRVAQPGDAYRVDALEPGMAPEDVITALSRCGECTP"
     gene            783..2627
                     /locus_tag="PKW60_01665"
     CDS             783..2627
                     /locus_tag="PKW60_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPJ98167.1"
                     /translation="MRHRATLTLHGLSEWQADPGAFSDTLAPFPVLQVDRSGRIVQAN
                     AAAKDAFGLAPAPLGSLAGIFPDAGRLRLPDCIDGGSERLFCAKIGDRRWQVSLVGDP
                     GNNTALLYAQDISSYEAMARDVAEIRQEQQRQQKELQCVYGLAEAISVRETAQEICRE
                     VVRLLPRAWRYPEHARGRIVLDGEEYVSEPFEMTQWGHSAAITVKGAARGMVQVFYTR
                     DCRHGDAEGPFLPEERRLLDVVARMLGEAIARRGAEAGNRAKALTLAQERNRLETILN
                     SMGEGVVVTDSQARIVMMNPAMGELLKRAPEACTGRDFLDLMPDGAFRALWRKAEADA
                     QDFAKERVVLGHPESRICWATQSCIHNMGAGETWRVTIFQDVTRELEIDQMKSDFVAA
                     VSHELRTPMTSIKGFVTTLLGKPNVKPELRERFLTIMDEEADRLITLIEELLLIARID
                     SGQVVLERDPVALVELAQCVLATLGHVAEGKKLRLSIHTENPLPPVYGDAKRLHAVLH
                     NLVENAIKFTPEGGWVRGRIFGQGASVVLEVTDNGVGIPREAQTKIFDRFYRVHREGE
                     VVPGTGLGLFIVQEMARLHDGRVEVQSEPGRGSRFRVFVPAAPGAAPE"
     gene            2649..3575
                     /locus_tag="PKW60_01670"
     CDS             2649..3575
                     /locus_tag="PKW60_01670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPJ98168.1"
                     /translation="MTHMERQYSALVVEDDVHTRELVVFNLEDAGYLVHSAGNGVEAL
                     ALLAERVVDIIICDIMMPEMDGFALREAVQLQARLRETGFIFLSAKALPEDEIRGLRY
                     GVDEYITKPFNPDVLVARVEAALARRTVLAKAARLDPLTQLYNRQAGERRIRKELERI
                     QRYPSIGALAFLDIDGFKAVNDRLGHAMGDRALLLLAEVLRETIRSVDIVARFGGEEF
                     VLFFPQTEEASAVQVIERMQRRFQQLSAIRTGLRISFSAGVVEAPRDGSQFDTLCRRA
                     DEAMYSAKERGKAQVTPWRPDIRGAGRFSRMP"
     gene            complement(3717..4673)
                     /locus_tag="PKW60_01675"
     CDS             complement(3717..4673)
                     /locus_tag="PKW60_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ98169.1"
                     /translation="MTTEPQRTPEVLRERRLALEREREAAMARRTRKDLYHNSPFYPE
                     FWLDKLVTLTGLRARGEQNARDFQLRTLTLAFENLPPAFDGFRVLLLSDMHFREEDPQ
                     FLQRLCEFLAPIQADFCAFTGDYRYAFTGSFDHVFESMQRLLESLPAHCDRAAVLGNH
                     DLTAFVRPFKDMGLPVLMNEHISLNRSGERLWIAGVDDPHRFRCDDLAAATHGIPFDS
                     FTILLAHSPELARRAPRHGIDLYLCGHTHGGQVCLPGGRPILLNARAARKYCAGLWQN
                     GRTRGYTTAGLGTTDVPVRFYCPPEAVLIQLHRASQESAPGA"
     gene            4935..7607
                     /gene="aceE"
                     /locus_tag="PKW60_01680"
     CDS             4935..7607
                     /gene="aceE"
                     /locus_tag="PKW60_01680"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HPJ98170.1"
                     /translation="MKNGHQPNETVDIETREWLESLDFVIENGGPERVLHLLQHLQIR
                     AEEAGIKLPFSANTPYINTIHVTQQPVYPGKREIERRIKSLIRWNAMAMVVRANRLEP
                     DIGGHISTYASAATLYEVGFNHFFHGPDTPGQSGDQVFFQGHAAPGFYARAFLEGRLS
                     AVQMNNFRRELAEGGGLSSYPHPWLMPNFWQFPTVSMGLGPIMSIYQARFNRYLEDRG
                     LKENTGRVWAFLGDGECDEPETLGAISLAAREKLDNLTFVINCNLQRLDGPVRGNSKI
                     IQELEAIFRGSGWNVIKVVWGSDWDPLLAQDKYGLLVERMGETIDGEYQKFVVESGEY
                     IRERFFGKDPRLLKLVESYTEEQIRRLNRGGHDPQKVYAAYHAAVNFRGAPTVILAKT
                     VKGYGLGESGEGKNITHQQKKLNEDELREFRTRFGIPISDEDVALAPFYRPPEDSPEM
                     VYLRERREGLGGYLPSRSVQVEPLAPPEESFFEEFYKGTGGRPVSTAMAFVRILTKLL
                     RDKRLGRYIVPIVPDEARTFGMEALFREVGIYAHTGQLYEPVDSASLLYYKEAKDGQI
                     LEEGITEAGSMSSFISAGSAYATHGLNMIPFFIYYSMFGFQRIGDLIWAAADMRTRGF
                     LVGGTAGRTTLSGEGLQHQDGNSHILAQPVPNLVAYDAAQAYELAVIVREGMRRMYEA
                     QESIFYYLTVENENYPMSPMPEGVKEGILKGMYKFKASEKRDSKLHAQLLGSGAILNE
                     ALKAQEILEQDYGVAADVWSVTSYKELRRDALDVERWNMLNPGKKPQVPYVTRCLKGE
                     PGVVVAASDYIKLLPDGVLRWVPQTMLTLGTDGFGRSESREALRDFFEVDARHIALAA
                     LTGLAREGKVKPDALKKAVKKLDIEPDKRNPLYT"
     gene            7683..9047
                     /locus_tag="PKW60_01685"
     CDS             7683..9047
                     /locus_tag="PKW60_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPJ98171.1"
                     /translation="MATEFRLPELGENIEFGKVTSVMVSVGDSITKDQSVIEVETDKA
                     ALEVPSSVSGTVTEIRVKEGDNVKVGAVVLVVEAGGAAPKPAAPAAAKAEEPAAPEVK
                     QPEPPAPKPEPPQPVEEAAAPVAPAETAPAAPAETVPVEATERQLTAAAVAASPSVRR
                     LAREIGVDVNQVPGTGKGGRIMPEDVKAYARRMNVDAGGVASPRAPVGDAAGFPLPDF
                     ARWGVIERRAMSTVRRRTAERLGHAWSQIPHVTQFDKADVTGLEQLRKQYARQVEAAG
                     GKLTVTAILAKVVAAALRKFPQFNSTLDFAHDEIIYKQYCHIGIAVDTDRGLLVPVLR
                     DVDTKNLTQVCVELSQLAERARTKKTTLDEMQGGCFTISNLGGIGGTAFTPIVNEPEV
                     AILGVSRNAVEPVFINGEFQPRTMLPLSLSYDHRVIDGADGARFLRWLCEAIENPFLV
                     FLEG"
     gene            9052..9609
                     /locus_tag="PKW60_01690"
     CDS             9052..9609
                     /locus_tag="PKW60_01690"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011586185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPJ98172.1"
                     /translation="MHTRNPWKLQSSRLVYETPWLRVIEDQVVRPDGADGIYSYIETR
                     LAVGAVALTPEHEVYLVGQYRYPTREYSWEIVEGGTEPGEEALACIQRELQEEAGLRA
                     AAWTPLGGEIHFSNCISAERGLLYLAEDLETTEVSPEGTEVLEIRRVPLRDAVAMVER
                     GEIKDVFSIVGLLRAQAFVRQRGAD"
     gene            9688..11169
                     /locus_tag="PKW60_01695"
     CDS             9688..11169
                     /locus_tag="PKW60_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPJ98173.1"
                     /translation="MKTRSVAVLDVGKTNKKVRLYDRQFQPLAEERTSFEVQMRDGLE
                     IEPTEELLAWFEEALARLGAQYDIRAIAVCAHGATLALLDEAGKLAYPVISYTSEKGA
                     EIEQEFYDAFGDEKSLHYDTCTPNVGFANCGKVLFYVFNRMPEVWDKVKTILFYNSYL
                     GYELTGNFGMEPTYLGNHSYLWNFFDGTWSPVAIRMGAHTRFPASLGSPWQSLGRVKA
                     ELAKRCNLPEDCQVTLGIHDSNANFLPYLAKGHTNFILNSTGTWCVLMSQSPTPLLTE
                     AEVDAKVFCNLDVFNRPVKTVIFPGGMEYEKFGALTEVRDESTVDDIKRIIAERRVFV
                     VPGVMPAASAFPGVPAKVIVDGKTRLYTDLEAEGGTPYTPLGQTYYAALNLSLALATR
                     QLLERCGGEKGASVFVEGGFAHNQRYCELLAALCPDYTIALTSQAEGTAFGAALTAWM
                     LADGQKLAQIGEEFEIETTPVVAPDFGDLQGYWDAFECHVRKA"
     gene            11215..12645
                     /gene="lpdA"
                     /locus_tag="PKW60_01700"
     CDS             11215..12645
                     /gene="lpdA"
                     /locus_tag="PKW60_01700"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPJ98174.1"
                     /translation="MSEPLKTQVAVIGGGPGGYAAAFAAADLGLEVTLIDLEKNPGGV
                     CLYRGCIPSKAFLHCAKILKDAEEAAEWGIYFEKPRLELDKLRAQKNAVVEKLTAGLG
                     QLTRARKIRFLRGRAAYQDATTLKVTADTGEEQFIECEHSILATGSRPTSVPSLLIES
                     PRMMNSTAALELNEVPKTLLVVGGGYIGLELGSVYAALGSRVTLVEMTPSLLPMVDED
                     LRTILAERVGGMFHKTLFNTRVEKVKEVKNGLRVTFRDGDGAVFEETFERVLSSVGRT
                     PNSSGLGLNTTGVELDERGFVKVDAQRRTNVPSIFAIGDLAGNPMLAHKASHEGRVAA
                     EVIAGHNSVFAPRAIPAVVFTDPELAQCGITEIQARKEGRPVKVARFPWGASGRATTL
                     SRPDGLTKLITDPDTDQVLGMGIVGTGAGELISQGVLAIEMGATAEDIGLSIHPHPTL
                     SETVMETADMIFGQSVHMYRPKGKDS"
     gene            complement(12781..13665)
                     /locus_tag="PKW60_01705"
     CDS             complement(12781..13665)
                     /locus_tag="PKW60_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPJ98175.1"
                     /translation="MRPAAPRPVSAARSGFTLIELLVVIGIIGILAAILLPALSKARA
                     QARGVQCVSNLRQLYLANTMYANEHNGCYVPAAFDINNWLPGGNLHRWHGVRPAADQP
                     FDPKRGPLAEYLADGRVKECPEFTEFRDSSNAGFEAFESGTGGYGYNASYVGGAYYRV
                     DFMIAPEHTTLDSRVFRPSETIMFADCAMPKNNGIIVEYGFAEPPHFVTDDHPTGNLT
                     WGLATPSLHFRHNGRVNVVWCDGHISSEKFGWTAANIYNGNNAQHAIGWFGPENNYYF
                     DCGDKSAYALAGGGATPP"
BASE COUNT         2665 a         3648 c         5044 g         2494 t
ORIGIN      
        1 gaccgccgat gtgctgctcg acgcgggcgc ggcagcgctc aacatcatcc ccgatcgcaa
       61 ctggaacgtg aaaagccccg atgaaaggca cgcgaaggtg gagaaactgc acgagatcgt
      121 cgcgattgcg gaagagcgcg gattgcccgt cctggcgggc accgagatga atgcgcatgg
      181 gcagaagttt gtggacgagt tcggcgtgcc ggagatggcg ccgctgcgcg agaccttcat
      241 gaatggagcg ctgatcctgt acgcgcatac gctgctggaa cgcgccgggg gaatggggta
      301 cctgagcgac tgggcgaaac ggcattttgc gtctccgcgg gaccgaaacg cgtattttgt
      361 cgagttggga cgagtagcgc agcctggaga cgcgtatcgg gtggatgcgc tggagccggg
      421 gatggcgccc gaggatgtta taacggcctt gagccgctgc ggcgagtgca ctccgtgagc
      481 agtcccgcac gtgcatttcc agaaagcggg gaagaattcg ctgatttgta cgctgcgcgc
      541 tgtccggagg gagtggctac gggccggcag ggagtgaggg aggctggccg gggggttcct
      601 ttttcaggat atttgtggta actttatttt tgtcaatggt ttagttcggc cggtcggggc
      661 gttgtccggc aggcaatctg cgtccatcgg ccgttactgt taaaattcgt agtgtgggcg
      721 ttttccaatc gccgcggatc agggtgctca atccgcagga tgaaggagcg tagccggcta
      781 cgatgcggca cagggctaca ttgacgcttc acggactgtc cgagtggcag gcggaccccg
      841 gtgcattttc tgacacgctg gccccatttc ccgtgctcca agtcgaccgt tcggggagga
      901 ttgtgcaggc gaacgccgca gcgaaagacg cgttcgggtt ggcgccggcg ccattggggt
      961 ccttggcggg gatattcccc gacgccggcc ggctgcgcct gccggactgc atcgatgggg
     1021 ggagcgaacg gctcttttgc gcgaaaatcg gcgatcggcg gtggcaggtg tcgctggtcg
     1081 gcgaccccgg gaataacacg gctctcctgt acgcccagga catctcgtcc tatgaagcca
     1141 tggcgcgcga cgtggcggag attcggcaag aacagcagcg gcagcagaag gaattgcagt
     1201 gcgtctacgg cctggcggaa gcgatctccg tgcgggagac ggcgcaggag atctgccgcg
     1261 aggtggtgcg cctgctgccg cgagcctggc ggtaccctga gcatgcgcgt ggacggatcg
     1321 tgctggatgg cgaggaatac gtgagcgagc cgttcgagat gacgcagtgg gggcactcgg
     1381 cggccatcac ggtgaaaggg gcggcgcgcg ggatggtgca ggtgttctac acccgggatt
     1441 gccgccacgg ggatgcggag gggccgtttc tgccggagga gcggcgcttg ctggatgtgg
     1501 tggcgcgcat gctgggggag gcgatcgcgc gccgtggcgc ggaggctggc aaccgggcca
     1561 aggcgctgac cctggctcag gagcgcaatc gcctggagac catcctgaac agcatgggcg
     1621 aaggcgtcgt ggtgacggac agccaggccc gcattgtgat gatgaatccg gcgatggggg
     1681 aactgctcaa aagggccccc gaggcctgca cgggcaggga ttttctcgat ttgatgccgg
     1741 atggggcgtt ccgggctttg tggaggaaag ccgaggcaga cgcccaggat tttgccaaag
     1801 aacgtgtcgt gctgggccat cccgagtcgc ggatctgctg ggcgacgcag tcgtgcatac
     1861 acaatatggg tgcaggcgag acctggcgcg tgacgatctt tcaggacgtg acgcgcgagc
     1921 ttgagatcga ccagatgaag tcggatttcg tcgcggccgt gtcgcatgaa ctccggactc
     1981 ccatgacgtc gatcaaggga tttgtgacga cgctgctggg caaacccaac gtgaagccgg
     2041 aactgcgcga acgctttctg accatcatgg acgaagaggc ggatcggctg atcacgctga
     2101 tcgaagagct gctgctgatc gcgcggatcg attcgggcca ggtggtgctg gaacgggatc
     2161 ccgtggcgct ggtcgaactg gcccagtgcg tgttggccac gctgggccac gtcgcggaag
     2221 gcaagaagct gcgcctctca atccacaccg agaatccatt gcccccagtc tacggcgacg
     2281 cgaagcggct gcatgcggtg ctgcataacc tggtcgagaa cgccatcaaa ttcacgccgg
     2341 aagggggctg ggtgcgcggg cgcatttttg gacagggcgc cagcgtggtc ctggaggtga
     2401 cggacaacgg cgtcgggatc cctagagagg cgcaaacgaa gatcttcgac cggttttacc
     2461 gggtgcatcg cgaaggggaa gtggttccgg gcacggggct gggcttgttt atcgtgcagg
     2521 agatggcgcg gcttcatgac ggccgggtgg aggttcagag cgaaccgggg cgcgggtcaa
     2581 ggttccgggt ctttgtgccg gctgccccgg gcgccgcacc ggagtgattt agtcgcaggg
     2641 aggcggccat gactcatatg gagcggcagt actcggcgct ggtggtggag gatgacgtcc
     2701 atacgcgtga actggtcgtg ttcaacctcg aggacgcggg gtatctcgtg cattcagcgg
     2761 ggaacggcgt ggaggcgctg gcgctcctgg cggagcgcgt ggtggacatc atcatctgcg
     2821 acatcatgat gccggagatg gacggctttg cgctgcggga ggcggtgcag ttgcaggcga
     2881 ggctccggga aacggggttc atttttctgt cggcgaaagc actcccggaa gatgagatcc
     2941 gggggctgcg ctacggggta gacgaataca ttacgaagcc atttaaccct gatgtgctgg
     3001 tggcacgggt ggaggcggca ctggcacggc ggacggtgct cgcgaaggcg gcgcggctgg
     3061 atccgctgac gcagttgtac aaccggcagg cgggcgaacg ccggatccgc aaagagcttg
     3121 agcgcatcca gcgttacccg tccattggcg ctctggcctt tctggacatc gacggattta
     3181 aggccgtcaa cgataggctc ggacacgcga tgggcgaccg ggcgctgctc ttgctggcgg
     3241 aagtgctgcg ggagactatc cggagcgtgg atatcgtggc gcggttcggc ggcgaagagt
     3301 tcgtcctgtt tttcccacag accgaggaag cgagcgcggt gcaggtgatc gagcgcatgc
     3361 agcgccggtt tcagcagttg tcggctatcc gtaccggcct gcggatctcg ttcagcgccg
     3421 gggtggtgga agcgccacgc gacggcagcc agtttgatac gctgtgccgc cgggcggacg
     3481 aggccatgta ctcagcgaaa gagcgtggga aggcgcaggt gaccccctgg cggccggata
     3541 ttcgaggcgc cgggcggttt tcgcgcatgc catgaagggc gaacatccgg ggaacccgcc
     3601 tgtgattttt cgatcggagc cgagtatctc tgcgttcggg cggtagcgcg ttgcagcgcg
     3661 ggcagagcgc cagggaaccg gtccttcttt tccccgcctg aggcggcaga cctaaactat
     3721 gctcctggtg cggattcttg cgaagcccgg tggagctgga ttaaaacggc ttcgggcggg
     3781 cagtagaacc gcacggggac gtcggtagtg ccgagtccag cggtggtgta gccgcgggtg
     3841 cgcccgtttt gccacagccc agcacagtat ttccgcgctg cgcgcgcgtt gagaaggatg
     3901 gggcggccgc cgggaaggca tacctggccg ccgtgcgtat ggccgcagag atacagatcg
     3961 atgccgtggc gcggggcgcg ccgagcgagt tcggggctgt gggcaagcag gatggtaaag
     4021 ctgtcgaagg ggatgccgtg cgtagcagcg gccagatcat cgcagcggaa acggtgggga
     4081 tcgtcgacgc ccgcgatcca gagccgctcc ccgctgcgat tcagggagat atgttcgttc
     4141 atgaggacgg ggaggcccat gtccttgaac gggcgcacga atgcggtgag gtcgtggttg
     4201 ccgaggacgg cggcgcgatc gcagtgggct gggaggctct cgaggaggcg ttgcatgctc
     4261 tcaaaaacgt ggtcgaagga gccggtgaag gcgtaccggt agtcgccggt gaaggcgcaa
     4321 aaatcggcct gaatgggggc gagaaattca catagtctct ggagaaattg ggggtcttct
     4381 tcgcgaaaat gcatgtcgct gaggagaaga acgcggaatc cgtcgaaagc cggggggagg
     4441 ttttcaaacg cgagggtgag ggtgcggagc tggaaatcac gggcgttttg ttctccccgg
     4501 gcgcgcaggc ccgtgagcgt cacgagcttg tcgagccaga attcaggata gaacgggctg
     4561 ttgtggtaga gatctttccg ggtgcgccgg gccatggcgg cctcgcgctc gcgttcgagc
     4621 gccaggcgtc gttccctgag gacctccggg gtgcgctgcg gttctgtggt catggacgag
     4681 gttttgggtt cttttctgtg cctgttgttg cgctcgatag tacgaatgcg ggatgaagga
     4741 tgcaacgtgc gggccgggtt ggggcgccgg gcgtgccggc gggaatttgg ccgagacttt
     4801 gatcgttgaa ctacagggac ttacaatgac ggcgcgacag gacgtgggca ctgtgttgcc
     4861 ggcggggcgc gggaatggtt ttgggatgcc ggaaggcagg gcgttttttg agaaagcagg
     4921 ttgtaatgga acccatgaag aacggtcacc agccaaacga aacagtggac atcgaaacgc
     4981 gggaatggct cgaatccctg gattttgtga ttgagaatgg cgggccggag cgtgttctgc
     5041 atttgctgca gcatcttcag atccgggccg aggaagccgg catcaagctt ccattcagcg
     5101 cgaatacgcc atacatcaac acgatccacg tcacgcagca gccggtctat cccggcaagc
     5161 gggagattga gcggcgcatc aagagcctga tccgctggaa cgcgatggcg atggtggttc
     5221 gcgcgaaccg gctggaaccg gacatcggcg gccatatctc gacgtatgcc tcggcagcga
     5281 cgctgtacga agtggggttc aaccatttct ttcatggccc ggatacgccg gggcagagcg
     5341 gcgaccaggt gtttttccag ggacatgcgg cgccggggtt ctatgcgcgg gcgtttctgg
     5401 aagggcgctt gagcgcggtg caaatgaaca atttccgccg ggaactggcg gaaggcggcg
     5461 ggctgtcgtc ttacccgcat ccgtggttga tgccgaactt ctggcagttt ccgacggtat
     5521 cgatggggct gggcccgatc atgtcgatct accaggcgcg gtttaaccgg tacctggaag
     5581 atcggggcct gaaggaaaac acggggcggg tatgggcgtt tctgggcgat ggggaatgcg
     5641 acgagccgga aacgctcgga gcaatttccc tcgcggcccg ggagaagctc gataacctga
     5701 cttttgtgat caactgcaat cttcagcggc tggatgggcc ggtgcggggc aacagcaaga
     5761 tcatccagga gctggaggcg atattccggg gttcgggctg gaacgtaatc aaggtggtct
     5821 ggggttcgga ctgggatccg ctgctggcgc aggacaagta cggcctgctg gtcgaacgga
     5881 tgggcgagac catcgacgga gaatatcaga agttcgtggt ggaatccggc gagtacatcc
     5941 gggagcggtt tttcggaaaa gatccgcgtc tgctcaaact ggtggagagt tatacggagg
     6001 agcagatacg gcgcctgaac cggggcgggc acgatccgca gaaggtctac gcggcgtatc
     6061 acgcggcagt gaatttccgg ggcgcgccga cggtcattct ggcgaagacg gtgaagggct
     6121 acgggctggg cgaaagcgga gaaggcaaaa acatcaccca ccagcagaaa aagctcaacg
     6181 aggacgaatt gcgggagttc cggacgcggt tcgggatccc gatttcagac gaagacgtgg
     6241 cgctggcgcc gttctaccgg ccgccggagg acagcccgga gatggtgtat ctccgtgagc
     6301 ggcgcgaagg tctcggaggc tacctgccga gccggagcgt gcaggtggag ccgctggcgc
     6361 cgccggaaga gagctttttc gaagaattct ataaggggac gggcgggcgc ccggtatcaa
     6421 cggcgatggc gtttgtgcga atactgacca agctgcttcg cgataagcgg ctgggacggt
     6481 acatcgtacc catcgtgccg gatgaggcgc ggaccttcgg catggaagcg ctgttccgtg
     6541 aggtcgggat ttatgcgcac acgggccagt tgtacgagcc ggtggactcg gccagcctct
     6601 tgtactacaa ggaagcgaaa gacgggcaga tcctcgagga aggcataacg gaagcgggtt
     6661 cgatgtcgtc gtttatcagc gcgggttcgg cgtacgcgac gcacggactc aacatgatcc
     6721 cgtttttcat ttactactcg atgttcgggt ttcagcggat cggcgacctc atctgggcgg
     6781 cggcagacat gcgcacgcgc ggcttcctgg tcggcgggac cgcgggccgg acaacgctga
     6841 gtggagaggg cctgcaacac caggacggga acagccacat cctggcgcag ccggtcccga
     6901 atctggtcgc gtatgacgcg gcgcaggcgt atgaactcgc ggtcattgtc cgcgagggga
     6961 tgcggcgcat gtatgaagcg caggagagca tcttctatta cctgacggtg gagaacgaga
     7021 attacccgat gtctccgatg ccggaaggcg tgaaagaggg gattctcaaa gggatgtaca
     7081 agttcaaggc gtcggagaag cgcgatagca agcttcacgc acagctcctg ggcagcggcg
     7141 cgatcttgaa tgaggccctg aaggcgcagg aaattcttga gcaggattat ggtgtggccg
     7201 ccgacgtgtg gagcgtgacg agttacaagg aactgcgccg cgacgcgctg gatgtggagc
     7261 gctggaacat gctgaatccg ggcaaaaagc cgcaggtgcc gtacgtcacc cggtgcctga
     7321 agggagagcc cggcgtggtg gttgcggcgt cggattacat caagctgttg ccggacggcg
     7381 tcctgcgctg ggtcccgcag accatgctga ccctggggac ggacggattt ggccggagcg
     7441 agtcgcggga ggcgcttcgc gatttcttcg aggtggacgc gcggcacatc gcgctggccg
     7501 ccttgaccgg tctggcccgc gaaggaaaag tgaagccgga cgcgttgaaa aaagctgtca
     7561 agaagctcga cattgagcca gataagcgga atccgctgta tacctgatcc tcagcgggat
     7621 ctgaccggat tttcaggcgg tattgggcag taaatcgcgg cagttgcaaa aaggacaaaa
     7681 cgatggctac ggaattcaga cttcccgaac ttggcgaaaa catcgaattc ggaaaggtca
     7741 ccagcgtgat ggtgtcggtg ggagactcca tcacgaagga tcagtccgtg atcgaggtgg
     7801 agaccgacaa ggcggcgctg gaggtgccgt cgagcgtgag cggcacggtc acggagatcc
     7861 gggtgaaaga gggggataac gtcaaggttg gggcggtcgt cctggtcgtt gaagcgggtg
     7921 gcgcggcgcc gaagcccgcg gcgcccgccg cggcaaaggc ggaggagccg gcagctccgg
     7981 aggtcaaaca gcccgagccg cctgcaccga agcctgaacc gccacagccg gtggaagaag
     8041 cagcggcacc cgttgcccct gccgaaaccg cacctgcggc gccggcagag acggtcccgg
     8101 tggaagcgac ggaacggcag ttgactgcgg ccgcggtggc ggcgtcgccg agtgtgcggc
     8161 ggcttgcgcg agagatcggc gtagatgtga accaggtgcc gggcacgggc aagggcggac
     8221 ggatcatgcc ggaggacgtg aaagcgtatg cgcggcgcat gaacgtggat gcgggaggcg
     8281 tggcgtctcc acgcgccccg gtgggcgatg ccgcgggatt cccgttgccc gattttgcgc
     8341 ggtggggggt gatcgaacgg cgcgccatga gcacggtacg gcgccggacc gcggagcggc
     8401 tggggcatgc gtggtcgcag atcccccacg tgacgcaatt tgacaaggcg gatgtgaccg
     8461 ggcttgaaca gctgcggaaa cagtatgccc ggcaggtcga ggcggcgggc gggaagctca
     8521 cggtcacggc gatcctggcg aaagtggtgg cggcggcgct tcggaaattc ccgcagttca
     8581 acagcacgct tgatttcgcg cacgacgaga tcatttacaa gcagtattgc cacatcggga
     8641 ttgccgtcga cacggaccgg ggcctgctgg taccggtatt gcgcgacgtg gatacgaaga
     8701 acctgacgca ggtctgtgtg gaattgagcc agctggcgga acgcgcccgg acgaaaaaga
     8761 cgacgctgga cgagatgcag ggcgggtgct tcacgatttc aaatctgggc ggcattggcg
     8821 gcacggcgtt cacgccgatc gtgaacgagc cggaagtggc gatcctcggc gtgtcgcgca
     8881 acgcggtcga accggtcttt atcaacggag aattccagcc gcgaaccatg ctccccttaa
     8941 gcttgtcgta tgaccaccgg gtgatcgacg gtgcggatgg cgcacgtttt ctgcggtggt
     9001 tgtgcgaggc catcgagaat ccgttcctgg tttttctcga gggctgaggc cgtgcacacg
     9061 cggaatccgt ggaaactcca gtcgagccgc ctggtctacg aaacaccgtg gctgcgggtg
     9121 attgaggatc aggtcgtgcg tccggatggc gccgacggca tctacagcta catcgagacc
     9181 cggctggccg tgggagctgt ggcactgaca ccggagcatg aggtgtatct agtgggacag
     9241 taccggtacc ccacccgcga gtattcgtgg gagattgtgg aaggcggtac agaaccgggc
     9301 gaagaggcgc tggcctgtat ccagcgggag ctgcaggaag aggcgggcct gcgcgctgcg
     9361 gcgtggacgc ctctgggcgg cgagatccac ttcagcaact gcatctcggc agaacgaggc
     9421 ctgttgtatc tggcggagga cctcgaaacg accgaagtct ctccggaagg cacggaagtg
     9481 cttgagattc ggcgggtgcc tctccgggat gcggtagcca tggtggagcg gggggagatc
     9541 aaagatgtgt tcagtatcgt cgggctgctg cgcgcgcagg cgtttgtaag gcagcgggga
     9601 gcggattaac cgttttctcg tccgtcttgc tatgctttgc gggcaaaggt gcagcagcaa
     9661 ggtaagagtt cagggggagg atgtgctgtg aagacgcgct ctgtggccgt gctcgacgtg
     9721 ggcaaaacga acaagaaggt ccggctgtac gaccggcaat tccagccgtt ggcggaagaa
     9781 cgcacgagtt ttgaggtgca gatgcgcgac ggtctggaaa ttgagccgac ggaagagctg
     9841 ctggcgtggt ttgaggaagc actcgcgcgc ctgggcgcgc agtacgacat ccgggccatc
     9901 gccgtgtgcg cgcacggcgc gacgctggcg ctgctggacg aggccggaaa actggcctac
     9961 ccggtgatct cgtacacgag cgagaaaggc gcggagatcg agcaggaatt ttacgacgca
    10021 ttcggcgacg agaagtcact gcattacgac acctgcacgc cgaacgtggg ctttgccaat
    10081 tgcgggaaag tgctgtttta cgtattcaac cggatgccgg aggtctggga caaggtcaag
    10141 acgatcctgt tctacaactc gtaccttggc tatgagctga cgggcaattt cgggatggag
    10201 ccgacgtatc tcggaaacca cagctatctc tggaattttt ttgacgggac gtggagcccg
    10261 gtggcgatcc gcatgggggc gcacacgcgg tttcctgcat cgctgggaag cccgtggcag
    10321 tcgctcgggc gagtgaaagc cgagctggcg aaacggtgca acctgccgga agattgccag
    10381 gtgacgctgg gcattcacga ttcgaacgcg aattttctgc cgtacctggc caagggccac
    10441 accaacttca ttctgaactc aacgggcacc tggtgcgtgc tgatgagcca gtcgccgaca
    10501 ccgctgctga cagaggccga ggtcgacgcg aaagtgttct gcaatctcga tgtgtttaac
    10561 cggcccgtga aaaccgtgat cttcccggga gggatggagt acgaaaaatt cggggcgctg
    10621 acggaagtgc gcgacgaaag caccgtggac gacatcaaac gcatcattgc cgagcggcgg
    10681 gtgtttgtcg tgcctggcgt gatgccggcg gcgagcgcgt tccccggagt gcccgccaaa
    10741 gtgatcgtgg acggcaagac gcggctgtat acggatctgg aggccgaagg cggcacgcca
    10801 tatacgcctc tggggcagac gtattacgcg gcgctgaacc tgagcctggc actggcgaca
    10861 cggcagcttc tggagcgctg cggcggagag aaaggcgcat cggtatttgt cgagggcgga
    10921 tttgcgcaca accagcggta ttgcgagttg ttggccgcct tatgcccgga ttacacgatc
    10981 gcgttgacca gccaggcgga aggcacggcg ttcggagccg cgctcacggc ctggatgctg
    11041 gctgatggac agaaactggc gcaaatcggc gaagagttcg agattgaaac cactccggtc
    11101 gtggcgccgg attttggcga tctgcaggga tactgggacg cctttgaatg tcacgtgcgg
    11161 aaggcttgat gtgggatagt atgagccggt ccgtacaaca ccaggaagaa gactatgagt
    11221 gaaccgctta agacccaggt ggccgtgatt ggcggcgggc cggggggata tgccgctgca
    11281 tttgccgcgg ccgatctggg cctggaagtg acgctgatcg atctggaaaa gaatccgggc
    11341 ggggtttgcc tgtaccgggg ctgcattccg tcgaaggcgt tcctgcattg cgcaaaaatc
    11401 ttgaaagacg ccgaagaggc ggcagaatgg ggcatttatt tcgagaaacc ccggctcgag
    11461 ctcgacaagt tgcgcgcaca gaagaacgcg gtggtcgaaa agttgacggc agggcttggc
    11521 cagcttaccc gggcgcgcaa aatccggttc ctgcggggcc gggcggccta tcaggatgcg
    11581 acaacgctga aggtaacggc ggacacgggc gaggagcaat tcatcgaatg cgagcactca
    11641 attctggcga ccggttcgcg cccgacgtct gttccgagcc tgttgattga atcgccgcgg
    11701 atgatgaatt cgacggcggc cctggagctc aatgaggtgc cgaaaacact cctggtggtc
    11761 gggggcggct acatcggcct ggaactgggc agcgtgtatg cggcgctggg gtcgcgggtg
    11821 acgctggtcg agatgacgcc gtcgcttctt cccatggtgg atgaagattt gcggacgatc
    11881 cttgcggaac gcgtgggcgg gatgttccac aaaacgctgt ttaatacgcg cgtggagaag
    11941 gtcaaagagg tcaagaacgg tctgcgcgtc acgttccggg atggtgacgg ggcggtgttc
    12001 gaagagacgt tcgagagggt gctgtccagt gtcggcagga ctcccaattc gagcggactg
    12061 ggcctgaaca ccacgggggt tgagctggac gaacgcggat ttgtgaaggt agatgcacag
    12121 cggcggacga acgtcccgtc gatctttgcg atcggcgatc tggcgggcaa cccgatgctg
    12181 gcgcacaagg cctcacacga agggcgtgtg gcggcggaag ttatcgcggg gcataattcc
    12241 gtgttcgcgc cgcgtgcgat tccggcggtg gtatttaccg accccgagct tgcgcagtgc
    12301 ggcattacgg agatacaggc gcggaaagaa gggcggccgg tgaaggtggc gcgcttcccc
    12361 tggggcgctt ccgggcgcgc gaccacgctc agccggccgg atggcctgac gaaactgatc
    12421 accgatcccg acacggatca ggtgctgggc atgggcattg tgggcacggg tgcgggcgaa
    12481 ctgatctctc agggcgtgct ggcgatcgag atgggcgcga ccgccgagga tatcgggttg
    12541 agcatccacc cgcatcccac gctgagcgag accgtcatgg agacggccga tatgatcttt
    12601 gggcagagcg tgcacatgta ccgcccaaag gggaaggaca gctagcgcgc ctgataaccg
    12661 catgaaacgg cggtcacgca ggccaggccg gagcctctgg gagagggtca gccccgcgtg
    12721 tacccgccct gccggcaggc ccaggccggt gcggcagacg gaatctccgg agccgtggac
    12781 ttacggcggg gtcgccccac ctccggcgag ggcataggca ctcttgtcgc cgcaatcgaa
    12841 ataatagttg ttttccggcc cgaaccagcc gatggcatgc tgcgcgttat tgccgttgta
    12901 gatgttggcg gcggtccatc cgaatttttc gctggagatg tggccgtcgc accagacgac
    12961 gttgacgcgc ccgttgtgcc gaaaatgcag gctgggagtg gcgagccccc aggtgagatt
    13021 gccggtgggg tgatcgtcgg tgacgaaatg gggcggttcg gcgaatccgt attctacgat
    13081 gatcccgtta ttcttcggca tggcgcaatc ggcgaacatg atggtctcgg agggccgaaa
    13141 cacgcgggaa tccagggtgg tgtgctcggg ggcgatcatg aaatcgaccc ggtaatacgc
    13201 gccgccgacg taagaggcgt tgtagccgta gccaccggtc cccgactcaa acgcctcgaa
    13261 gccggcgttg ctgctgtcgc ggaattcggt gaattcgggg cattccttga cgcgcccgtc
    13321 ggccaggtat tcggcgagag gcccccgctt cggatcgaag ggctggtcgg cggccggccg
    13381 gacgccgtgc cagcggtgca ggttgcctcc ggggagccag ttgttgatgt cgaaggcggc
    13441 gggcacgtag cagccgttgt gctcgttggc gtacatggtg ttggcgaggt agagctgccg
    13501 gaggttgctg acgcactgga cgccgcgtgc ctgggcgcgc gccttgctga gcgcgggcag
    13561 gagaatcgcg gcgagaatgc cgatgatccc gatgacgacc aggagttcga tcagggtgaa
    13621 tccggaacgg gcggctgaca cggggcgggg ggcagcgggt ctcatagcgc gtgaggtcct
    13681 ttcccgggga cgcgatgcgt ccgcggcagt ggcgacgcac accagacagg cggcatgccg
    13741 ggaatggcct cggggaaagg aagggaagga agcgtaacgg gcattcctgc gcaacccttt
    13801 ccgcgaagtt gccgcttgtg atcgtgtgcc cgtgggctgg ttttctggct t
//
LOCUS       DAOPBQ010000024        13804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4456_length_13804_cov_4.202487, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000024 DAOPBQ010000000
VERSION     DAOPBQ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 13804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13804
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4456_length_13804_cov_4.202487"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3137)
                     /locus_tag="PKW60_01710"
     CDS             complement(<1..3137)
                     /locus_tag="PKW60_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPJ98176.1"
                     /translation="MLAIAISCAVVFVCLGWMRQARAETLDWENPALLGRNKEPYHCT
                     LLPYPDAASALEGTRDASPFHQSLNGTWKFHWVGQPADRPRDFFKPEYDVSHWGEIPV
                     PSVWQLQGYGIPIYTNVRYPFPPNPPHIPQDYNPVGSYRRVFEIPETWRGRRIFIHFD
                     GVESAFYLWLNGTEVGFSKNSLGPAEFDITSFLKDGENTLAVEVYRWSDGSYLEDQDM
                     WRFSGIFRDVYLFAAPETHVRDFFVRCRFDGEYRDASLQLTAKVRNYGARTGGAHTLE
                     AVLLDADGAPAEKAPLMATEFGPLAAGEEQVLELEALVKAPRHWSAEDPYLYTLLLEL
                     KTPSGETVEVVSCRFGFRDVAIRDAQLLINGVPVKLKGVNRHEHDPWRGRAITHERMV
                     QDIELLKRFNFNTVRTSHYPSDPKWYELCDRYGIFLIDEANLESHGMGYDLNSTLGNR
                     PDWEAAHVDRHTALVERDKNHPSVIIWSLGNEAGSGCNFEAGARAIRALDPTRPIHYE
                     RMNDVADIDSVMYPHLDDLIAEGKKDSPKPFIMCEYAHAMGNAVGNLEEYWEAIETHD
                     RLIGGCIWEWADHGIFKQTEDEPREDGSRPWFWAYGGDFDDQPNDGNFCMDGLVFPDR
                     SIPPKMWEVKKVYQYVAVAPVDLARGRVRIRNKHFFVNLDRYELRWGIREDGRIVEES
                     ALPPLDLPAGQETELSIPFAPPSPKPGAEYTLRISFHLRDKTLWAEAGHEAAWEQMRL
                     PVESGKRPAATFGCGSNLKVQEGDTLMTVSGAGFTVRFSKSHGSIATIVYDGQEILPR
                     GGAVFHGPVLNLLRAFTDNDTRLIGGESLKRDFYAAGLSQMRFYLKRFEVTQENGEMA
                     RITARLDCLGSKGRGFMHECVYTIYPDGSIHLRNHLEPQGELPMLPRIGLKMVLAGEL
                     EHLAWYGRGPHESYPDRKTGAALGKYKGTVTAQYVPYPFPQECGNKEDVRWASLTNEA
                     GKGVLLVMDSPLSFSALRFMPCDLNRATHPSDLKPRRNITVCVDYRHCGLGNASCGPP
                     VLEKYALYASTVDFG"
     gene            3321..6713
                     /locus_tag="PKW60_01715"
     CDS             3321..6713
                     /locus_tag="PKW60_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPJ98177.1"
                     /translation="MSWRSVLLVVLLAAVAWCGEAAEQLWHFQTPLGNFDSSPAVADI
                     NGDGHQDVILTTVSGSVVVIDSAGREVWGRSLQIPISLPPTVADLVEDERLEILVLNQ
                     VGTLFCLDGQSGDVIWKYDLPASIEWGSTAITVYDLDGDGHLEVIAGDSAGHVVCLSA
                     VGERLWQYDGDHGYTLSPAVGTLSGSEAPSIFISGSKAPLIRLDASGKEVWRLGIDAK
                     GAAPILADINNDGTREILTAIDNAVVAVDANGEVMWRFEMPKEIDSSICVGDANQDGI
                     PEIYTIDLSGQIASIAPDGTKLWTGNLHARARRSPALADIDGDGAIEIVAAGYSGELF
                     VFNADGEIKDKIRMPNPTNASPTVADLQGNGTPCIVYACTSGGVLAWKWPTAQAGARV
                     LWPEYRFTSARAGALVTDGEQSRVRITAADFGSLYAGENAIQFDIDNPARRPLRVDIT
                     TSRSGEIPRTTSVEVSDPVATVTDRYSLPTGRTVDLQITCCVYDGETIVARRSHSAQI
                     VPLQKELSDLAALLDGVTVAAQHLPDAYAALGRASEVRERREHCAKRIAGITTLSDIE
                     QRELRDVVQRDIHDFSSLLKLMKTAAEHRDAGSWPLRLSAANPWAPFGAFDEILEGRL
                     EEPGVTIEAFNGEVEDAALNVFNLGTAPLQARVEIDPLVLEGASETAPVAPRDVIAIH
                     EVVSVPTQSLDLSADVLPRINSGYVLILPAWDARQLWFAVQTKDLAPGRWMSTIRIRT
                     LEVESREFTAPLTIDVWGAALPETQALRHCNWGYVYQTPRMQGFEDETIEDRVAHGNN
                     VFVTGLVPRATYDETGAFTGEIDYTQHDAYVRRYAPHGMILFHNTGGITGPGGIGTDA
                     YRKAFVAWMRAWVAHLKDMGLGYDQYAMYPVDEPGLEEGLVERYLFYAQLAREADPKI
                     LMYTDPVARIRKEELVEMLPYVDIWCPNLEGFLLDENQDKLEVIKNSGKTLWTYECLD
                     NAKHRSPLGYYRGQPWLAWHHKLSGIGFWSYCTSVADPWYKPEDTLDYLMVYPGDGVV
                     ISKRWEAVRDGIEDFDMLVVLRDAARKAQAENRAPEAVAKAETLLSESVLEIARFCGW
                     DEDGTTPGKEGLPGVRVVEDKRYAAIRAVRREMAQLLDALAP"
     gene            complement(6789..7931)
                     /locus_tag="PKW60_01720"
     CDS             complement(6789..7931)
                     /locus_tag="PKW60_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPJ98178.1"
                     /translation="MTRRERLMKTLAGQPVDRPPVCFYELNGIDEEPDDPDPFNVFND
                     PSWRPLLDLTRDRTDRIVLRSVPFQDKPPDPLEPFRTFEQWEESGCRLERTTLRAGGR
                     TLTTLNRQDEGVYTIWTVEHLLKDLDDFKAWLALPQEEFGGTPDIAEVLMTEDLLGDA
                     GIVCLDTPDPLCHVAPLFDMGTYLLIAMTENALMHQALEKVASLLLPRVEAVARALPG
                     RLWRIFGPEYASPPYLPPNLFEEYAARYVTPMVEAIQRHGGYARVHSHGRLRDILDHI
                     AATGCCGLDPIEPPPQGDVTLAYVRERYGAQMALFGNLEVSDIENLPTPQFAEKVATA
                     LREGTAGTGRGLVVMPSAAPYGRVISPLTLRNYEEMVRQAEAFSGA"
     gene            8049..10970
                     /locus_tag="PKW60_01725"
     CDS             8049..10970
                     /locus_tag="PKW60_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPJ98179.1"
                     /translation="MRLTFTWPCRISYLLCSCALFAGIIIFSSQAAPAKEPDSSTGGA
                     IGGPGANLSTVARERSFRDQVEADWLFQEQYRYGALSQEITPESDAAGACDGVKDGGW
                     GFHTDRQDTPWWQVDLESEYPIQRIVVWNRCDAVAPRAAHLQVLASVDGSSWQAWYAH
                     DGTVFYGFSDNAPLVIDIGGKPARFVRVQLPGEEFLHLDEVEIFGADEPGENLALHRP
                     ALQSSLSQWSIYHLPPASIDWDARLARVVAHSAGLLDELEMEGIDVSAERALLASLAR
                     REGSQEACFEARWLQRRLTLAHPILSFGTIFFAKRQPGTYNHMSDQYYGWWSRPGGGL
                     YLLRNFAHPDGPAIECLSEAMGGGGSFLRPILSYDATRALFAWCRYYPGLAEQPDKLN
                     KDNVPEDAFYHVFEMDLATRQVRQLTRGKYDDFDARYLPGGDIIFLSTRRGQALQCGK
                     DSARCTLETPDLPDAYVRCGGGPERPVAVYTLHRMNADGGDLCAISPFEMFEWTPSIA
                     HDGRILYSRWDYVDRDNMPYMGLWSINPDGTNSSIVYGNFTHAPHCTFEPRSIPNSRK
                     IIFTASGHHAQTMGSLVLLDPSVGTEGTAPITRLTPEVVFPEIEGWPETYFANPWPLS
                     ERFYLVAWGRVENMREGVTMPGNAMGLYLFDADGNLELLHRDPDFSCAWPMPLEPQPK
                     PLQRPDAPEWDRAQEGRFVMTDVYQGLPNTPRGTVKALRIVAVPVKTHPTMNSPNLGV
                     TNDDPGKCVLGTVPVEADGSAHFRAPAGVMVFFQALDEHGIALQSMRSSTHVQPGQTL
                     SCVGCHEPRTMAPPATRAVAARREPSKIAPGPEGSWPLRFDRLVQPVLDANCVACHRA
                     DSEHPGAAQRPLTAEASYDMLVAFGEPSLREHVQERYAKGESLEGQGAAASSPLLAML
                     TRPDGHHGVKLAPAHLERLIVWMDTYAQRLGSFDERQEQQLAALRAQWHDLLAPSPES
                     R"
     gene            11128..12462
                     /locus_tag="PKW60_01730"
     CDS             11128..12462
                     /locus_tag="PKW60_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98180.1"
                     /translation="MQSRFSRRAFLKQSSLALTAPLILPRLSLGQSPNSRLQLAAIGV
                     GGKGASDLDSIVSSRKVDVIALCDVDETRLNEAARKHKDARTYVDWRELFAQEADHID
                     AVCVATPDHMHAPIAYSAISLGKHCYCEKPLTQEVYEARQLRLAAERKGVVTQMGNQI
                     HAHASYRNAVKMMQDGVLGRVKEWHSWISNAYGGKDLVRPVTEDPLPSGLHWDLWLGV
                     APPRPFKAELYHPRLWRFWQDFGSGALGDFGCHIFDPVFSGLGIGAPLRLTAETPEFA
                     DGVWPYWEVIRYEFPATAMAAGDTICATWYDGGKRPGPEAMGLPAGVPLPDAGSVIVG
                     EEGTMVLAHYGKPILLYPEDKFKEFTQPELPSLNHYHQWVNACLGTDTAASSFSNASP
                     LVEAVLLGNVANRCAGQTLEWDAPNLQFTNAPEANALLRVPYREGWEVEGLS"
     gene            12508..12699
                     /locus_tag="PKW60_01735"
     CDS             12508..12699
                     /locus_tag="PKW60_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98181.1"
                     /translation="MLYTFATLSTAQLTAIQQLEQEEGLKILALTAVETEPDAIDAAR
                     LSKLQAVEAQLGCCLIAVR"
     gene            complement(12696..>13804)
                     /locus_tag="PKW60_01740"
     CDS             complement(12696..>13804)
                     /locus_tag="PKW60_01740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPJ98182.1"
                     /translation="TPSGTPADTKPTNSGTAALHLAVAACGCGGGDEVIVPAYCWPSS
                     AACVLHHHAIPVFVDIDYDTMNIDPSRIEAAISERTKAIIAAHLHGLPASMDPILEIA
                     VRHGLRVIEDCCQAHGAEYKGTRVGRLADCGAFSFNQSKCLCSGEGGMFVTDNEEILQ
                     RARQAWSFGGPGGLGEGTGPFAYALGWMYRNNDLTAAFGRAQLAKLDAYLETAARNAK
                     ILADGVRDIPGLQPPIAPEGHRTNWYNFVCHVDGTAHGYQGPPERFRNAIVKALQAEG
                     VPVDVWQDFILPNMAVFQAQRDFSDGQPRNPAYDPAQFPKAQKHCETHMGLTVPLRAP
                     NDELVAQLVGAGIRKVFEGIGALDIDVLGAETTA"
BASE COUNT         2567 a         4290 c         4351 g         2596 t
ORIGIN      
        1 aacccgaaat cgaccgtgga cgcgtacaaa gcgtattttt ccagcacggg cgggccgcaa
       61 ctcgcgttcc ccagtccgca atgccggtaa tccacgcaga ccgtaatatt ccgccgcggt
      121 ttcaggtcac tggggtgtgt cgcgcggtta aggtcgcagg gcatgaaccg caatgcgctg
      181 aacgagagcg gcgaatccat gaccagcagc acgccctttc ccgcctcgtt cgtgagcgaa
      241 gcccagcgca catcttcctt gttgccgcat tcctgaggga agggataggg cacatactgc
      301 gcggttaccg tgcctttgta ttttccgagg gccgcgccgg ttttacgatc agggtagctt
      361 tcgtgcggac cgcgtccgta ccaggccagg tgttcaagtt cgcccgccag caccattttg
      421 aggccgatgc gcggcagcat gggcagctcc ccctggggct caaggtggtt ccgcaggtgt
      481 atggagccgt ccgggtagat cgtatacacg cactcgtgca tgaagccccg gcccttgctt
      541 cccaggcagt ccagacgtgc cgtgatgcgc gccatttctc cattttcctg ggttacctcg
      601 aagcgtttca ggtagaaacg catctggctc aggcccgccg cgtagaagtc ccgcttgaga
      661 ctctcgccgc cgatgagccg ggtgtcgttg tcggtaaatg cgcgaaggag gttcagtacg
      721 ggcccgtgga atacggcgcc gccgcggggc aggatctcct gtccgtcgta gacgatggtc
      781 gcgatactgc catgagactt gctgaagcgg accgtgaatc cggcgccgga gaccgtcatc
      841 agcgtgtcgc cttcctggac tttcagattg gagccgcagc caaacgtggc tgcgggtctt
      901 ttgcccgatt caaccggcag gcgcatctgt tcccaggcgg cctcatggcc tgcttccgcc
      961 cacagggtct tgtctcgcag gtgaaagctg atccgcagcg tgtactcggc accgggtttc
     1021 ggtgacggcg gggcaaaggg gatgctgagt tctgtctcct ggccagcggg cagatccagc
     1081 ggcggcaagg cgctctcttc cacgatgcgc ccatcttcgc ggatgcccca ccgcagctcg
     1141 tagcgatcca ggttcacgaa gaaatgcttg ttgcggatgc gtaccctgcc gcgggccaga
     1201 tcgacggggg cgacagccac gtactggtag accttcttga cctcccacat cttgggcggg
     1261 atgctgcggt cgggaaagac cagaccgtcc atgcagaagt tgccgtcgtt gggctggtcg
     1321 tcaaaatcgc cgccgtaggc ccagaaccag ggccgcgaac cgtcctcgcg gggttcgtct
     1381 tccgtctgct tgaaaatgcc gtggtcggcc cactcccaga tgcagccccc aatcaagcgg
     1441 tcgtgcgtct cgatagcctc ccagtattcc tccagattgc ccacggcgtt gcccatggcg
     1501 tgcgcgtatt cacacatgat gaacggcttg ggtgaatcct tcttcccttc ggcgatgaga
     1561 tcgtccagat gggggtacat cacgctgtcg atatccgcca cgtcgttcat gcgctcgtag
     1621 tggatggggc gcgtggggtc gagggcgcgt atggcgcggg cgcccgcctc aaaattgcag
     1681 ccgctgcccg cttcgttgcc cagcgaccag ataatgaccg acgggtgatt tttgtcacgt
     1741 tccaccagcg ccgtatgccg gtcgacgtgc gcggcttccc aatccggccg gtttccaagc
     1801 gtgctgttga ggtcgtaccc catgccatgg gattcgaggt tggcctcgtc gatgaggaag
     1861 atgccatagc ggtcgcacag ttcgtaccac ttggggtcgc tggggtagtg gcttgtgcgc
     1921 accgtattga aattgaaacg cttgagcagt tcgatgtcct gcaccatacg ttcgtgcgtg
     1981 atggcccgcc cgcgccaggg gtcgtgttca tgccggttga cgcccttgag ctttacgggc
     2041 acgccgttga tcagcagctg ggcatcacgg atcgccacgt cgcgaaatcc gaaacggcag
     2101 gataccacct ccacggtttc tcccgatggc gtcttgagct cgagcagaag cgtgtagaga
     2161 tacgggtctt cggctgacca gtgccggggt gccttcacca gagcttcgag ttccagaact
     2221 tgctcttcgc cggccgcaag gggcccgaat tccgtggcca tcaacggcgc cttctctgca
     2281 ggcgcgccat cagcatccag gagcacagcc tccagcgtat gggcgccgcc ggttcgcgca
     2341 ccgtagttgc gcactttggc agtaagttga agcgaggcgt cgcggtattc gccatcaaac
     2401 cggcatcgga cgaagaaatc gcgcacgtga gtctcgggcg cggcaaacag gtagacatcg
     2461 cggaaaatgc ccgaaaaccg ccacatgtcc tgatcttcga ggtagctgcc atccgaccag
     2521 cggtacacct caacggcgag ggtgttttca ccgtctttca gaaacgaggt gatgtcgaat
     2581 tcagcgggac ccaggctgtt tttgctgaaa ccgacctctg taccgttcag ccagagatag
     2641 aaggcgctct cgacgccgtc gaaatggatg aaaatacgcc tgccgcgcca cgtttcgggg
     2701 atttcaaaga cgcgccggta cgaacccacg ggattgtagt cctgggggat gtgcggcgga
     2761 ttcggaggaa acggataccg cacgttggtg tagatgggga tgccgtaacc ctgaagctgc
     2821 cataccgacg gcaccgggat ctcgccccag tgggagacgt catactccgg tttgaaaaaa
     2881 tcgcgagggc ggtcggcggg ctggcctacc cagtgaaact tccacgtgcc attcagcgat
     2941 tggtggaacg gcgaggcgtc gcgcgtgccc tccagcgcgg atgctgcgtc gggatacggc
     3001 agcagtgtgc agtggtacgg ttccttgttg cgcccgagca gggccgggtt ttcccagtcc
     3061 agcgtttcgg cgcgagcctg ccgcatccac cccagacata cgaacacgac agcgcaggaa
     3121 atagcaatgg cgagcacggc agtctcccaa cagttgcagc ttttcagggt tgtctacccg
     3181 gcaccacacc acagcctgtg tctgtattgt cgcgagcttg ccgatgaaat tcaaccacct
     3241 cagcttgaat acgcgcggcc gggtgcttat tgtaggccgt tcgacccaaa cactgtccaa
     3301 tcgtttcagg agtgcatggc atgagttgga gatctgtcct tctggttgtg ttgctggctg
     3361 ccgtcgcgtg gtgtggcgag gcagcggaac agctgtggca ttttcaaacg cctctgggca
     3421 atttcgacag ctccccggcg gtagccgata taaacggcga cggacatcag gatgtgatct
     3481 tgaccaccgt gtcagggagc gtggtggtga ttgacagcgc agggcgcgag gtctggggcc
     3541 ggagcctgca gatccccatc agcctgccgc ccaccgtggc ggatctggtt gaagacgagc
     3601 gcctggaaat ccttgtgctg aatcaagtcg gtacgctgtt ctgcctggac ggccagagcg
     3661 gagatgtaat ctggaaatat gacttgcccg catcgatcga atgggggagc accgcgatta
     3721 cggtgtacga tctcgatggc gacgggcacc tcgaagtgat tgccggcgat tcggcgggtc
     3781 atgtggtctg cctgtctgcg gtgggcgagc gcctttggca atacgatggc gaccacggat
     3841 acaccctctc gcccgccgtc ggaacgctgt ccggatccga ggcgccatcg atctttatct
     3901 cgggttccaa ggcgccgctg atccgcctgg atgccagcgg gaaagaagtg tggcgcctgg
     3961 gtatcgatgc caagggtgcc gcgccgattc tcgccgacat caacaacgac ggcacccgcg
     4021 agatcctgac ggcaattgac aacgccgtcg tggccgttga cgcgaacggc gaggtgatgt
     4081 ggcgattcga gatgcccaag gagatcgaca gcagcatctg cgtcggtgat gcaaaccagg
     4141 acggcatccc ggagatctac acgatcgacc tgtccgggca gatcgcgtcc attgctcccg
     4201 atggcaccaa actctggacc ggcaaccttc acgcgcgggc gcggcgatcc ccggccctgg
     4261 cggacatcga cggtgacggc gccatcgaaa tcgtggccgc cggttacagc ggcgaactct
     4321 tcgtgttcaa cgcggacggc gagatcaaag acaagatccg gatgccgaat ccgacgaatg
     4381 cctcgccgac ggtcgccgat ctgcagggta acggcacgcc ctgcatcgtc tatgcctgca
     4441 catctggagg cgtgttggcc tggaaatggc ccaccgccca agccggtgcg cgggtgctct
     4501 ggcccgaata ccgcttcact tccgcgcgcg cgggcgcgct ggtgacggac ggagagcaat
     4561 cccgcgtacg cattaccgct gcggatttcg gttccctgta cgcgggagaa aacgccattc
     4621 aattcgacat cgacaatccg gcccggcgcc cgctgcgcgt cgacatcacg acttcacgga
     4681 gcggtgaaat accgcgcacg acatccgtgg aggtgtcgga cccggtggcc accgtaaccg
     4741 atcggtattc actgccaacc ggaaggacgg tggacctgca gataacgtgc tgcgtgtacg
     4801 atggggagac gattgtggcc cggcgttcgc atagcgccca gatcgtgccc ctgcagaaag
     4861 aactctccga tctggccgca ctgctggacg gtgtgacggt ggccgcgcag catcttcccg
     4921 atgcgtatgc cgccctgggc cgcgccagcg aagtcaggga acggcgcgaa cactgcgcga
     4981 agcggatcgc gggcatcaca accctgtcag acatcgagca acgcgagctc agagacgtgg
     5041 tgcagcggga cattcatgat ttttccagct tgctcaagct tatgaagacc gccgcggagc
     5101 atcgcgacgc gggctcctgg cctctgcggc tgagcgcagc caatccctgg gcgcctttcg
     5161 gcgcgtttga cgagattctg gaggggcgac tggaagagcc cggggtgacg atcgaagcgt
     5221 tcaatggcga ggtcgaagac gcggcgctca acgtgttcaa tctcggtacc gcgcccctgc
     5281 aggcgcgcgt tgagatcgat ccgctggtgc tcgaaggcgc gtcagagacg gcgcccgttg
     5341 ccccacgcga tgtcatcgcg atccacgagg tagtgagcgt gccgacgcag tcgctggatc
     5401 tctccgcgga cgttctgccg cgcatcaact ccggctacgt cctgattctg cccgcctggg
     5461 atgcgcggca actgtggttt gcggtccaaa cgaaagacct ggcccccggc cgctggatga
     5521 gcacgatccg gatacgcacg ctcgaagtcg agtcgcggga attcacggcc ccgctcacca
     5581 tcgacgtctg gggcgccgcc ttacccgaga cacaagcgct cagacactgc aactgggggt
     5641 acgtgtacca gactccgcga atgcagggtt ttgaggacga gacgattgag gatcgcgtcg
     5701 cacacgggaa caacgtgttc gtgacggggc tcgtcccgag agccacctac gacgagacgg
     5761 gcgcgtttac cggagagatt gattacactc aacacgacgc ctacgtgcgc cgttatgcgc
     5821 ctcatggcat gattttgttt cacaatacgg gcgggatcac gggcccggga ggtatcggga
     5881 ccgatgccta tcgaaaggcg tttgtggcct ggatgcgtgc ctgggtggcc cacctcaagg
     5941 acatgggcct cgggtacgac cagtatgcca tgtatcccgt cgatgaaccc gggttggagg
     6001 aagggctggt cgagcggtac ctcttctacg cgcagctcgc ccgggaagcc gatccgaaga
     6061 tcttgatgta taccgatccc gtggcgcgga ttcgcaaaga agagctggtc gagatgctgc
     6121 cgtacgtcga catctggtgc ccgaatctgg aaggattcct gctcgacgag aatcaggaca
     6181 agctggaggt tatcaagaac tccggcaaga ccctgtggac ctacgaatgc ctcgataatg
     6241 cgaaacaccg ctcgccgctg ggctattacc ggggacagcc ctggctggcc tggcatcaca
     6301 agctctcggg catcggattc tggtcgtact gcacctcggt ggcggatccc tggtacaaac
     6361 ccgaagatac cctcgattat ctgatggtgt acccgggtga cggcgtcgtt atcagcaaac
     6421 gctgggaagc cgtgcgcgac ggcatcgaag atttcgatat gctcgttgtg cttcgcgacg
     6481 ccgcacggaa ggcccaggcc gaaaaccgtg ccccggaagc ggtcgcgaag gccgagacgc
     6541 tcctttcaga aagcgtcctg gagatcgcgc ggttctgcgg atgggatgaa gacggcacta
     6601 cccccggcaa ggaagggttg cccggagtcc gggtggtgga agacaagcgg tatgcggcaa
     6661 tccgtgcggt ccggcgcgaa atggctcagt tactggacgc gctggcgccg tgaacaccgg
     6721 ccgcgcgagc ggtcaggcag tgagtgcatg aaacggagag cccggcggtt tccgccgggc
     6781 tttttccatc atgcgcctga aaacgcctcc gcctgccgca ccatttcttc gtaattccgc
     6841 agggtcaatg gcgaaattac gcgcccgtac ggggcggcgc tcggcatcac gaccaggccg
     6901 cggcctgtgc cagccgtccc ttcgcgcagg gccgtggcga ccttttcggc gaactggggc
     6961 gtgggcaggt tttcgatgtc cgacacttca agattgccga agagcgccat ctgcgcgcca
     7021 taacgttcgc gaacataggc gagcgtcacg tccccctgcg gcggaggttc aatcggatcg
     7081 aggccgcaac agccggtagc ggcgatgtgg tcgaggatat cgcgcaatcg cccgtgggaa
     7141 tgtacgcgcg cgtagccgcc atgccgctga atcgcctcca ccatgggcgt gacatagcgg
     7201 gccgcgtatt cttcgaagag attggggggc aagtagggcg gcgacgcgta ttccgggccg
     7261 aagatccgcc agaggcggcc cggaagcgcg cgtgccacgg cctccacgcg cggcaggagc
     7321 agcgacgcca ccttctcgag cgcctggtgc atcaacgcgt tttctgtcat tgcgatgagc
     7381 aggtaggtgc ccatatcgaa gaggggcgcg acgtggcaca gcggatccgg cgtgtcaaga
     7441 caaacgatcc ctgcatcgcc cagcagatct tccgtcatca ggacttccgc gatgtcaggc
     7501 gtgccgccaa actcctcctg cggcagcgcg agccatgctt tgaaatcgtc gagatctttc
     7561 aacaggtgct cgacggtcca gatcgtatat acgccctcgt cctgacggtt cagcgtcgtc
     7621 agggtcctgc cgccggcgcg cagggtcgtg cgttcaagcc ggcatccgct ctcttcccac
     7681 tgttcaaagg tgcggaacgg ttccagcggg tccgggggct tgtcctgaaa cggcacactg
     7741 cgcagcacga tgcgatcggt ccggtcacgg gtcaggtcca gcagaggtcg ccaggacgga
     7801 tcgttgaaca cgttaaaggg atcggggtcg tccggctcct cgtcgatgcc attcagctca
     7861 tagaagcaga cggggggccg gtcaaccggc tggcccgcca gggtcttcat cagtcgttca
     7921 cgccgcgtca tgcagtcaga ctccccatac ttcggctctg aaagctggcc cacgataccc
     7981 taaacccact atcaaaccaa aagcggacga tgtatcatag ggccacaaac tcatggggga
     8041 cttcgcttat gcgcctgacg ttcacgtggc catgccggat atcgtatctt ctgtgctcct
     8101 gcgcgctatt cgcagggatc atcatcttct cttcgcaagc cgcgccggcg aaagaaccgg
     8161 attcttcgac aggaggcgcc atcggcggcc ccggcgcgaa cctcagcacg gtggcgcgag
     8221 aacgctcgtt tcgcgatcag gtcgaggccg actggctgtt ccaggaacag taccgttacg
     8281 gcgcgctctc ccaggaaatc actcccgagt cagatgccgc gggcgcctgc gacggcgtca
     8341 aggacggggg atggggtttc cataccgacc gccaggatac cccctggtgg caggttgacc
     8401 tggaaagcga gtacccgatt cagcggatcg tcgtctggaa ccgctgcgac gctgtcgcac
     8461 cacgggccgc gcatcttcag gtgctggcat cggttgacgg gtcgtcgtgg caggcatggt
     8521 acgcacacga cggcacggtc ttctacgggt tttcggataa tgccccgctc gtgatcgata
     8581 ttggaggaaa accggcccgt tttgtgcgcg tccagctgcc gggtgaggag tttctgcatc
     8641 tcgatgaggt ggagatcttc ggcgctgatg agccgggcga gaacctggcg ctgcaccgcc
     8701 ccgccctgca gagcagcctt tcgcaatggt cgatatacca cctgccgccg gcatcgatag
     8761 actgggacgc gcgtctggcg cgggtcgtgg ctcattcggc cggccttctg gacgagctgg
     8821 agatggaggg catcgacgtc tcggccgagc gcgccctgct ggccagcctt gccaggcggg
     8881 aaggttctca ggaggcctgt tttgaagcgc gctggcttca gcgcaggctc acgctggcgc
     8941 acccgatcct gtcctttggc acgatctttt tcgccaaacg ccaaccgggt acgtataacc
     9001 acatgtcgga ccagtattac ggctggtggt cccgcccggg aggcggtttg tatctcctgc
     9061 gcaacttcgc ccaccccgac ggccccgcca tcgaatgcct cagcgaggcg atgggcgggg
     9121 gtgggagttt tctgcgcccg atcctgtcgt acgacgcaac ccgggccctg tttgcctggt
     9181 gccgctatta ccccggcctc gcggaacagc cggacaaact caacaaagac aacgttcccg
     9241 aggatgcgtt ctatcacgtg ttcgagatgg atctggctac gcggcaggtg aggcagctga
     9301 ctcgaggcaa gtacgacgat tttgacgcgc ggtatctgcc gggcggcgac atcattttcc
     9361 tgtcaacccg gcgcgggcag gcactgcagt gcggtaaaga cagcgcccgg tgcacgctgg
     9421 aaacgccgga tctgcccgat gcctacgtgc gctgcggggg cggacccgaa cgccccgtag
     9481 ccgtctacac cctccaccgc atgaacgccg acgggggaga cctctgcgcc atttcgccct
     9541 tcgagatgtt cgaatggacg cccagcatcg cccacgatgg gcgcatcctt tattcccggt
     9601 gggactatgt cgaccgcgac aacatgccgt atatgggcct gtggtctatc aaccccgacg
     9661 gcacgaacag cagcatcgtc tacggcaact ttacgcatgc gccccattgc accttcgaac
     9721 cacggtcgat cccgaattcc cggaagatca tcttcacggc ctcaggacat cacgcacaga
     9781 ccatgggcag cctggtcctg ctcgaccctt ccgtgggcac ggagggcact gcgccgatca
     9841 cccgcctgac gccggaagtc gtttttccgg agatcgaggg ttggcccgag acgtatttcg
     9901 ctaatccctg gcccctgtcg gagcggttct atctcgtggc ctggggcagg gtggagaata
     9961 tgcgggaagg ggtcaccatg cccggcaatg ccatgggcct ctatcttttc gacgccgatg
    10021 gcaatctgga actgctccac cgtgatcccg atttctcctg cgcctggccg atgccgctgg
    10081 aaccgcagcc gaaaccgctc caacggcccg acgctccgga atgggaccgg gcccaggagg
    10141 gccgctttgt gatgaccgac gtgtaccagg gactgcccaa cacgccgcgt ggaacagtca
    10201 aagcgctccg tatcgtcgcg gttcccgtca agacccatcc gacgatgaac tctcccaacc
    10261 tgggcgttac aaacgacgat ccgggcaagt gtgtgttggg aacggtacct gtcgaggcgg
    10321 acggttcagc acatttccgc gcacccgccg gagtgatggt gtttttccag gcgctggacg
    10381 aacacgggat cgcgcttcag tccatgcgga gttccacgca cgtccaaccc ggccaaacgc
    10441 tcagttgtgt gggatgccac gagccgcgca ccatggcccc gccggcgacc cgggccgtgg
    10501 ccgcacgcag ggaaccttcg aaaattgcgc ccggcccgga aggctcctgg cccctgcgct
    10561 tcgaccgtct ggtgcagccc gtgctggatg cgaactgcgt cgcatgccac cgggcggaca
    10621 gcgagcatcc cggcgccgcg cagcgcccgc ttacggcgga ggcatcgtat gacatgctgg
    10681 tcgcgttcgg ggagcccagt ctgcgcgagc atgtgcagga gcgttacgca aagggagaat
    10741 cgctggaagg gcaaggcgcc gcggccagca gcccgctcct ggcgatgctc acccgcccgg
    10801 acggccacca cggcgtgaaa ctggctcccg cccacctcga acggctgatc gtgtggatgg
    10861 acacctatgc gcagcgcctg ggctcgtttg atgagcgcca ggaacagcag ctcgccgccc
    10921 tgcgcgccca gtggcacgac ctgctggcgc cgtctcccga aagcagatga cgaatgaagg
    10981 ccgccggaca gctgcccggc ggctgatttt tctcaatgca acgctgccca tgaccggagc
    11041 gctgccaact tccacgcatt ccattgaacc tcggggggac gtgttttata ctccatttca
    11101 gtgccacaat ttcttcggag acctcgcatg caatcacgtt tttcgcgccg agcctttctc
    11161 aagcagtcga gccttgcact gaccgctccc ctgatcctcc ctcgcctcag cctgggccag
    11221 tcgcccaaca gcagacttca actggccgca atcggcgtcg ggggaaaggg tgccagcgac
    11281 ctcgattcga tcgtttccag ccggaaagtc gatgtaatcg ccctctgcga tgtggacgaa
    11341 acccgcctca atgaagcggc acgcaagcac aaggatgccc gcacctatgt cgactggcgc
    11401 gagctgtttg ctcaggaagc tgaccacatc gatgcggtat gcgtggccac gcccgaccat
    11461 atgcatgccc caatcgccta ttcggcgatt tcactgggca agcactgcta ttgcgagaaa
    11521 cccctgactc aggaagtcta cgaagcccgc cagttgcggc tggccgccga gcggaagggc
    11581 gtcgtgaccc agatgggcaa ccagatccat gcgcatgcca gttatcgaaa cgcagtgaaa
    11641 atgatgcagg acggtgttct cggcagggtg aaagaatggc attcgtggat atccaacgcc
    11701 tacggaggca aggatctggt ccgcccggtg accgaagatc cgcttccttc gggattgcac
    11761 tgggatctgt ggctgggcgt ggcgccgccc cgcccgttca aggccgagct ttaccatccc
    11821 cgcctttggc gcttctggca ggacttcggc tcgggcgccc tgggcgattt tgggtgccac
    11881 atctttgacc cggtgtttag cggattgggc attggcgcgc cactgcgcct tacggcagag
    11941 actccggagt ttgctgatgg ggtgtggccg tattgggagg tcatccgcta cgaattcccg
    12001 gcaaccgcta tggcggccgg cgatacgatc tgcgccacgt ggtacgatgg cggcaaacgg
    12061 cccggcccgg aggccatggg cctgcccgcc ggcgtgcccc tccctgatgc cggctcggtc
    12121 attgtcggcg aagagggcac catggtgctg gctcattatg ggaagcccat cctgctgtac
    12181 cccgaagaca agttcaaaga gttcacacag cccgagctgc cgagcctcaa tcattaccac
    12241 caatgggtta acgcctgcct gggcacggat accgccgctt ccagcttctc gaacgcgagc
    12301 cctctggtcg aggcggtgct gttggggaat gtggccaatc gctgcgccgg gcagaccttg
    12361 gaatgggatg cgccaaactt gcagttcact aatgcgcccg aagccaatgc ccttctgcgg
    12421 gtaccctacc gggaagggtg ggaagtggaa ggactgtcct gaggccggct cgtgacggct
    12481 ccggacactg cgcagggagg tcacggcatg ctgtacacat tcgcaacgct cagcacggcg
    12541 caattgacgg cgatccagca gcttgaacaa gaggaagggc tcaaaatcct ggccctgacc
    12601 gccgttgaaa ccgagccaga cgcgatcgac gccgcccggt tgtcgaaact ccaggccgtg
    12661 gaggctcagt tgggctgctg cctgatcgcc gtgcgctagg cggtggtttc cgcgcccagc
    12721 acgtcgatat caagggcgcc gatgccttca aataccttcc ggatgcccgc gcccacgagc
    12781 tgggccacca attcgtcgtt gggcgcgcgc agcggcacgg tcaggcccat gtgcgtttcg
    12841 caatgctttt gtgccttcgg aaattgtgcg ggatcatacg ccggattccg gggctgaccg
    12901 tcagaaaagt cacgttgtgc ctgaaacacc gccatgttcg gcaaaatgaa atcctgccag
    12961 acatcgacgg gcaccccttc cgcctgcaac gctttcacaa tggcgttgcg aaaccgctct
    13021 ggcggaccct ggtatccatg ggccgtgcca tctacatggc agacgaagtt gtaccagttg
    13081 gtccggtgcc cttcgggcgc gatgggcggc tgcaacccgg gaatgtcgcg cacgccatcc
    13141 gcaaggatct ttgcgtttcg cgcagccgtc tcgagatacg cgtcgagctt ggccagttgc
    13201 gcgcggccga acgcagctgt caaatcattg ttgcggtaca tccagcccag cgcatacgcg
    13261 aacgggccgg tgccttcccc gaggccgccc gggccgccga aagaccaggc ctggcgcgcc
    13321 cgctggagga tctcctcgtt gtccgtcacg aacatgccgc cctcgccgga acacaggcac
    13381 ttgctctgat tgaagctgaa tgcgccgcag tcggccagac gcccgacgcg cgttcccttg
    13441 tattcggcgc cgtgggcctg gcaacagtct tcaatcacgc gcagcccatg ccggaccgcg
    13501 atctccagga tcggatccat actggccggc aacccgtgca gatgggcggc gatgatggct
    13561 ttggtccgtt cagagatggc cgcctcgatg cgggacgggt cgatgttcat ggtgtcgtaa
    13621 tcgatgtcca cgaacaccgg aatggcatga tgatgcagca cgcaggccgc cgacgaaggc
    13681 caacagtagg ccgggacgat gacctcatcg cccccgccgc agccgcaggc cgccaccgcc
    13741 aggtgcaatg cggcggtgcc gctgttcgtc ggctttgtgt ctgcgggcgt accgcttggc
    13801 gtga
//
LOCUS       DAOPBQ010000025        13419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4603_length_13419_cov_3.501946, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000025 DAOPBQ010000000
VERSION     DAOPBQ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 13419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13419
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4603_length_13419_cov_3.501946"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1502
                     /locus_tag="PKW60_01745"
     CDS             75..1502
                     /locus_tag="PKW60_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPJ98183.1"
                     /translation="MKRTVFALAGMALLSAFFGFASVETLSMPVGWTTAIVRTAYTSA
                     VLAAFFLLLPRWFGIPARIFLAMASGALAGWLFQRAGATSFAVDYLNLFGTVFILLLK
                     LVVTPLIFVSILCGVSGIGDPRRLGSVGFKAIAYYLGTTSIAILIGMLCVNAIRPGTG
                     RHDLQTAVTSNALTESSPEKSAGAHPTPGRLLQDTVLPTIIDNPIMAGRDPLSIIFLA
                     ILLGASLASLGDRALPALETFRSLDRALIQLVLWIMQLAPIGVFTLMSKAIAELGVEY
                     ILSLAWYCLTVVTGLGLHFALLMGLLLPLAARVSPLRFLKGFAPAIALSFSTSSSSAT
                     LPVTIECTTRRIGADENICNFMLPVGATLNMDGTALYQAVAVLFIAQVLGKDLSLAQQ
                     FGVFLTALMVSVGSAGIPAASIALMPLVLKQAGIGAEAIGIVIGVDRFLDMCRTVVNI
                     TSDSVGALVVSRSEGAVLQPHAAEE"
     gene            1509..2165
                     /locus_tag="PKW60_01750"
     CDS             1509..2165
                     /locus_tag="PKW60_01750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPJ98184.1"
                     /translation="MEQLRMIRHIVWDWNGTLLDDLELCLTVLHELTSARGIPPVTLE
                     TYRDTFTFPVIDYYKALGFDASPREFEEAAREWVRMYTGQLYSMSGLYAGAADTLARL
                     RDAGLRQCLVSAHQHDMLLEAVRHFGVFPYFDTIRGLHNHYAESKVDLGRQWLAEIAP
                     PRESILFIGDTVHDLEVAQALGVRCLLVAQGHQSESRLRATGAPVVASIRDVPAFLRQ
                     "
     gene            complement(2187..5291)
                     /locus_tag="PKW60_01755"
     CDS             complement(2187..5291)
                     /locus_tag="PKW60_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPJ98185.1"
                     /translation="MKITSLPAMLMVGALLCIGAHNAIAWVRLRERKVHLAFAVACVF
                     VAFYAAATAGLYSAATPLDGQFWQRLQMTSAVFAAIAFVWFIWLYTGQLSVYVRNTFS
                     IYYAISGVVLWLDRSRFSWPMDRPSIKQVDLPFGLQVVYQEMAPGPLVEITSAIGMVF
                     LVYILYACLRFYRTGDKGRARVLLWALTLFVLGAINDMSITVGTYKFIYIMEYCYFGM
                     VLLADHALAHDMMEAVFQQQGGHAGEGRYTRLFQHVDNVYFELSPEGRILDVSPVVQR
                     VMDLSPAEVIGRSVEEFYASNEQRESLCQRIRETGRVADFDVRLRLGRGREGDFAVSA
                     ALVGDSRGIPGSVMGTLRDVTKRKEKEQQLARAAGLYERMRTLAKALDDAESFSAGLN
                     EALHAALEVTGSQAGAIYVLEKGRLSLRARQNLPLAAATALEALPDQADDFWAALPLD
                     RSRTLGSLLWDSQALPELGEYQSALLTPCAGRNRVVGYLVLAMPSGTESRGAAGAENV
                     VASEIGMFIARSRFVESVQRAREEFERAQEFAHVGNWEFDVADKKLRGSRETGRIFGF
                     PEHLAEVTLDQFFEVLHPVDRERVATAIGRFIRGEADTYDVEYHITRQSDGAARVVHS
                     LAQVVEDEAGRLTKVRGTVQDITERTELQQQLLLAQRMESVGLLAGGIAHDFNNILSV
                     IMSYSDMAAESLEPTEPKYAMIHEIACAAERARHLTHQLLAFSRRQVMEMRVIDLNAV
                     LRDMAPMLGRLIGEGIELRLELDEQVRPIEADIAQIQQIMMNLAVNGRDAMPQGGVLA
                     IATRVVEVEDHEVRHPPSRPAPGTYSVIMVSDNGCGMTPEVLEHVFDPFFTTKAVGKG
                     TGLGLATVYGIAKQHGGYIWADSIPGKGTRFRLYLPAIDKPPEEPPRPKPLSGSARGN
                     ETILVVEDEEMVRNLTSNVLRKHGYTVLTAQDGEDALRVAESSGNAIDLLLTDVVMPR
                     MSGLELYEQLAPRVPGMKVLFMSGYANELVSDCVALGPDAVLLQKPFTVHSLTEEVRS
                     MLDG"
     gene            5566..7389
                     /locus_tag="PKW60_01760"
     CDS             5566..7389
                     /locus_tag="PKW60_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HPJ98186.1"
                     /translation="MTSVVIALLSGVGFIAAYHTYGRYLARKIFCIDPNAAVPSQELE
                     DGVDYVPTRKEVLFGHHFVSIAGTGPIVGPAIAIFWGWLPALLWVLLGSIFIGAVHDF
                     GALVVSLRSRGQSIGDIAGRMISPRARVLFLVVLFFVLMVVIAIFGLVIAVIFSIYPQ
                     SVLSVWVAMPVAVGVGFWIYRRGGHLLAPSVLALVILYAAVAIGVYWCPIDLSALLRM
                     PLYAGNEAGFFSGLGSVVVIWTGVLLVYCFIASVLPVWMLLQPRDYINSHQLLVALVL
                     LALGLLVARPDMVAPAINGNVPADAPLMFPFLFITVACGAVSGFHCLVSSGTSSKQLR
                     NEADAQFVGYGSMLVEGMLAVIVILACGAGLGLGVMEKGQTITGAAAWSAYYGGEWGA
                     MSGLARNVGAFIEGGGNLIAATGIPPRLAVGITAVMIACFAATTLDTATRLQRYIVQE
                     LADVVRVPALHNKYAATTVAVVTGGALALLPGPNGPGSGGLILWPLFGAINQLLAGLA
                     FLVVAFYLIRHNRPAWVLAAPLAIMVALPAWAMGINVSNWIAKHNWLLAAIGSAVLLL
                     EGWMVVEAALMWKSARGVLPEALPPLKPATASALDERERCG"
     gene            7443..8546
                     /locus_tag="PKW60_01765"
     CDS             7443..8546
                     /locus_tag="PKW60_01765"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPJ98187.1"
                     /translation="MKQAAVLISFLLLSAVAGAAADAPQRTLVLDPGEGNPRNSEGDF
                     IQLNDGRLLFIYTHFTGSASDHGAAFLASRVSTDAGASWSAEDAVVLPNEGGMNVMSV
                     SLLRLRTGAIALFYLRKNSTSDCRPMMRLSNDEAATWSEPVLCIEPVGYFVVNNDRVI
                     QLESGRLVIPAARHSLPGESFSGRGQAMCYLSDDDGATWFPSESILDAPEDSKSGLQE
                     PAVVALNDGRLMMLCRTDQGCQMRSFSGDGGITWSPPELTDIASPVSPATIERIPSTG
                     DLLMLWNDHRAIEPALAGKRTPFAAAISRDEGQTWENVRLLESDPDGWYCYTALEFVD
                     DAALLGHCVGDSTVGHLNRTQVLRVPIAWFYEN"
     gene            8717..9469
                     /locus_tag="PKW60_01770"
     CDS             8717..9469
                     /locus_tag="PKW60_01770"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPJ98188.1"
                     /translation="MPVKWIVTDLDGCVSPEESVAWDLGPFVEFARLSRAASAGESTL
                     APLTLCTGRPQPYVEVLMKILDIRAPAICENGAVLYSLHDNWARFGPGVTSEKTHRLR
                     AVRDFIETELLHEYPQAVMQFGKEAQLSVFSQDPAVLLAMQPRVEQFVRENGPELIIN
                     CSHFYLNLSLAGVDKGSSLRALFDELGVQPHEVAGIGDTVGDLPLRDAVGFFACPSNA
                     QDEIKAVADYISPEPTVSGLLDILALPEMQRG"
     gene            9492..10301
                     /locus_tag="PKW60_01775"
     CDS             9492..10301
                     /locus_tag="PKW60_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPJ98189.1"
                     /translation="MWMRALCIVGFGAMTMPAWAGAGASILPPPRHGGVYVVAHRGAH
                     DGVPENSLAACQKAIELGADFVEVDARQTQDGHFVNIHNSTVDAYAEGATGAVHDLTL
                     AEIQALDIGSRVGPEWAGTRIPTVDEVLALCKGKIGIYMDFKDGSWEDILALVKKHGM
                     ERQVLWYASNERLKKIREACPKCIIMPDPGPGANLKPLLEDLRPKVVAAVWRHYSESF
                     VKTCHEAGAIVIVDEHDPSSWEPALAWKSDGIQTDHPEKLIEALKKRGQTQ"
     gene            complement(10295..11410)
                     /locus_tag="PKW60_01780"
     CDS             complement(10295..11410)
                     /locus_tag="PKW60_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPJ98190.1"
                     /translation="MTLRERILTVYRGGIPDAVPFMLDLSHWFYHRNEMPWDLSAAHP
                     EPETALIAYHRKVGAGFYLPQLSAFYDVCYPGDVTASAAKSPDGTAITWAIDTPLGTL
                     ARTRRWEPQNYAWGITGWPLKSERELGILQYALSRRSYTPRWERYHAWARAVGDTGVI
                     YVPTGYSAMGHLLNYWMGVEGAIYATFEMQAAVREFVDAVNANILDCVDMLAESPAEI
                     ILMGDNFSSDIQPPHFFEEWSGAFYTEAIARLHRAGKCVAVHIDGRLRGALGMFRDTG
                     ADCADAVTPGQPGDLSPSGCRDEAGPGLILSGGVPPHLWLPDVGVAVFDRAVLDWLDL
                     RHASPRLIANAGDQVPPGADESRIHRMRELVETQGRY"
     gene            11623..13224
                     /locus_tag="PKW60_01785"
     CDS             11623..13224
                     /locus_tag="PKW60_01785"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPJ98191.1"
                     /translation="MNRTSSATTYRIVSGTLILLALLAGLSPETAAAPLRLPAVFDDH
                     AVLQRNVSVPVWGWAEPGETVAVSIAGQTAAATAGADGRWRVDLAPLAAGGPFELTVS
                     APSGSRTLSDILVGEVWLASGQSNMQWSIKLSDGWDAGQAHWANDKIRIAMVFREHSP
                     VPLDDLRGWTPWAPCNAETLDTCYNGEGFSGVSYYFAKYLQAELGVPVGIINTSWGGT
                     RIEPWTPPVGFEPIPALADITTQIRLNQPETAEYQQALKDAIEQVEAWLPAARTALAN
                     HTFAPALPSIASESGLNNEGRPTTLYNAMVAPLVPYANRGFIWYQGESNRGDGMLYRD
                     KMEALINGWRSVWNDNALACYFVQLAPFDYGKAPEALAEIWEAQSAATAIPGTGMAVI
                     NDIGNVKDIHPRNKDDVGKRLAWLALNKTYGKSDIVCEGPVFDRFEIVDNALRVYFKN
                     VKTLSTRDGAAPNWFQICGADGVFHEAAAVIDGASVVLTAADVTTPVAVRFAWDQCAE
                     PNLKNEAGLPASAFRAGNVPAGGAE"
BASE COUNT         2441 a         4220 c         4106 g         2652 t
ORIGIN      
        1 gctgggtccc cggatctatt ccgcggagga tcgcgcatca caatacaatg tgacggaagg
       61 agagcggctt cgtcatgaag cgcaccgtgt tcgcactggc cggcatggct ctgctcagcg
      121 cgttcttcgg gttcgcgtcc gtggaaacgc tgtcgatgcc cgttggctgg accacggcga
      181 tcgtgcgcac ggcctatacc agcgccgtgc tcgcggcatt ttttctgctc ctgccgcgct
      241 ggttcggcat ccccgcgcgg atctttctgg cgatggcctc gggggcgctt gccggatggc
      301 tattccaacg ggcgggagcc acgtcgttcg cggtcgatta cctcaacctt ttcggcaccg
      361 tgttcattct tctgctgaaa ctggtcgtca cgcccctgat ctttgtctcg atcctctgcg
      421 gcgtgtcggg gattggcgac ccgcgccggc tcggctccgt gggtttcaag gccatcgcgt
      481 actatctcgg caccacatcc attgccatac tgattgggat gctctgcgtt aacgccattc
      541 gtcccggcac agggagacac gacctccaga ctgccgtcac cagcaacgct ctgacggaat
      601 ccagcccgga aaaatccgcc ggggcccacc caaccccagg ccggctcctg caggataccg
      661 tgctgcccac aatcatcgac aatcccatca tggctggacg ggaccccctc tcgatcatct
      721 ttctggccat cctgctcgga gcgtcgcttg cctccctggg cgaccgcgcc ctgcccgccc
      781 tcgagacgtt ccgctcactc gaccgggcgc tgattcagct cgtactgtgg attatgcaac
      841 tcgcccctat cggggtcttc acgttgatga gcaaggccat cgccgagctg ggcgtcgaat
      901 acatcctttc actcgcctgg tactgcctga ccgtggtgac gggtctcgga ttgcatttcg
      961 ctctgctcat gggtctcctg ctgccgcttg ccgcgcgtgt ttcgcctctg cggtttctca
     1021 aaggctttgc tccggccatc gcgctgtcct tcagcacgtc gtccagttcg gcgaccctcc
     1081 cggtgaccat tgagtgcacc acgcggcgca tcggagccga tgagaacatc tgcaatttca
     1141 tgcttcctgt cggcgccacc ttgaacatgg acggcacggc actgtaccag gcggttgcgg
     1201 ttttgtttat tgctcaggtc ctgggcaaag atctcagcct ggctcagcaa tttggcgtgt
     1261 ttctcaccgc gctcatggtg tccgtcgggt ctgcgggcat accggcggca agtattgcgc
     1321 tgatgcccct ggttctgaaa caggccggca tcggagctga agccatcggg atcgtcatcg
     1381 gcgtggatcg cttcctggat atgtgccgca ccgttgtgaa tatcaccagc gacagcgtcg
     1441 gcgcgctcgt ggtatcgcgc tccgaaggcg ctgtccttca gcctcatgct gccgaggaat
     1501 agactttcat ggaacagctc cgcatgatcc gccatatcgt ctgggactgg aacggcacct
     1561 tgctggacga tctggaattg tgtctcaccg tcctgcacga actgaccagc gcccggggca
     1621 ttcctccggt tacgctggag acttatcgcg acacattcac gttccccgtg atcgactact
     1681 acaaagcgct ggggtttgac gcgtcgcctc gggaatttga agaagcggcc cgggaatggg
     1741 tccgcatgta caccgggcaa ctttactcga tgagcgggct gtacgcgggc gccgccgata
     1801 ccctcgcccg cctgcgcgac gccggccttc gccagtgcct ggtgtctgcc catcagcacg
     1861 acatgctgct ggaggcggtc cggcatttcg gcgtgttccc gtatttcgat accattcgcg
     1921 ggctccacaa ccattacgcc gaaagcaagg tcgatctggg acgccagtgg ctggctgaga
     1981 tcgcgccacc gcgcgaatcg attctgttta tcggcgacac tgtacacgac ctggaagtgg
     2041 cccaggcgct gggggtccga tgccttctcg tcgcccaggg acatcaatcg gaatcacggc
     2101 tgcgcgccac gggcgccccg gtcgtcgcgt ctatccgcga cgtgccggcc tttttacgcc
     2161 agtgaccggc tccggcgggc gcgccgtcat ccgtcaagca tgctgcgcac ctcttccgtc
     2221 agagagtgta cggtaaatgg cttttgcagc agcacggcat ccgggcccag cgccacgcag
     2281 tctgagacca gttcattcgc atagcccgac atgaacagca ccttcatccc cggcacgcga
     2341 ggcgcaagct gctcgtagag ctccaggccg ctcatgcggg gcatcaccac gtcggtaagc
     2401 agcagatcga tggcgttgcc gctggattcc gccacacgca gggcgtcttc tccatcctgg
     2461 gccgtcaaga ctgtatagcc gtgctttcga agcacattgc tggtgagatt gcgcaccatt
     2521 tcctcatctt caacgacgag gatggtctcg ttgccccgcg cgcttccgga cagtggtttt
     2581 ggacgcggcg gctcttccgg cggtttgtcg atcgccggca gatagagccg gaatcgggtt
     2641 cccttccccg gaatgctgtc cgcccagata tatcctccat gctgtttggc gatgccgtac
     2701 accgtggcca gcccgaggcc cgtgcctttg ccgacggctt tggtggtaaa aaacggatca
     2761 aacacatgct ccagcacttc cggggtcatg ccgcatccgt tatccgacac catgatgacg
     2821 ctgtaggtcc cgggcgcggg gcgcgagggt ggatggcgca cctcatggtc ttccacttcg
     2881 accacacggg ttgcgatcgc caggacgccg ccctggggca tggcgtcgcg gccgtttacg
     2941 gccaggttca tcatgatctg ttgaatctgg gcgatgtccg cctcaatggg gcggacctgt
     3001 tcgtccaatt ccagtctcaa ttcaatgcct tcaccgatca gccgcccaag catgggcgcc
     3061 atgtctctaa gcacggcgtt gagatcgatg acccgcattt ccatgacctg ccggcggctg
     3121 aacgcaagaa gttgatgagt caaatgccgc gcccgctccg cggcacacgc aatttcatga
     3181 atcatcgcgt atttcggctc ggtgggctcc aggctctcgg ccgccatgtc cgaatacgac
     3241 ataatgaccg acaggatgtt attaaagtcg tgtgcgatgc ccccggccag caggcccacc
     3301 gattccattc gctgggcaag cagcagctgc tgctgcagct ccgtgcgttc ggtgatgtcc
     3361 tgcaccgtgc cccgcacctt ggtgagccgt cccgcttcat cttcgaccac ctgtgccagg
     3421 gaatgcacca cccgtgccgc gccatcgctc tgccgggtta tgtggtactc cacatcgtag
     3481 gtatcggcct cgccccgaat gaaccgcccg atggccgttg caacgcgctc tcgatcgacc
     3541 gggtggagca cttcgaaaaa ctggtcgagc gtgacctccg ccaggtgctc gggaaatccg
     3601 aagatccggc cggtctctcg cgagccccga agcttcttgt cggcgacgtc gaattcccag
     3661 ttgcccacat gcgcaaattc ctgcgcgcgc tcgaattctt ctctggcccg ctgcaccgat
     3721 tcgacgaacc gggagcgggc aataaacatg ccgatctccg acgcgacgac gttttcggcg
     3781 cctgccgcgc cacggctttc cgtaccgctg ggcattgcca gaaccaggta ccccacaacc
     3841 cggttacgcc cagcgcaggg cgtcagaagc gcgctttgat attcgcccaa ttcaggcagg
     3901 gcctgcgaat cccaaagcag cgaacccagc gtccgggatc gatccagggg cagcgcggcc
     3961 cagaagtcgt ctgcctggtc aggcagcgct tccagcgcgg ttgcggccgc aaggggcagg
     4021 ttctggcgcg cacgcagcga cagccgcccc ttttcgagca cgtaaattgc ccccgcctgg
     4081 ctgccggtga cttccagcgc ggcgtgaaga gcctcattta atcccgcgct gaaggattct
     4141 gcatcatcaa gcgctttcgc cagcgttcgc atccgttcgt acaggccggc ggcacgtgcg
     4201 agctgctgct ctttctcttt gcgtttcgtg acatcccgca gcgtccccat cacgctgccg
     4261 ggaatgccgc gggaatcgcc aaccagcgcg gcggataccg cgaaatcccc ttcacggcca
     4321 cgccccagcc gcagccggac gtcaaaatcg gcaacgcggc ctgtctctcg gatgcgttgg
     4381 cacaggctct cgcgctgttc gttcgaggcg tagaattctt ccacgctccg gccaatgact
     4441 tccgcggggg acagatccat cacgcgctgc accacggggc tgacgtccag aatacggcct
     4501 tccggactca gctcgaagta aacgttgtcc acgtgctgga agaggcgcgt gtagcggcct
     4561 tcgccggcgt gtccgccctg ttgctgaaat accgcttcca tcatgtcgtg cgcaagtgca
     4621 tggtcggcca ggaggaccat gccaaagtag cagtactcca taatatagat gaatttgtac
     4681 gtacccacgg tgatcgacat atcattgatc gccccaagca cgaacaaggt cagagcccac
     4741 aacaacacac gcgccctgcc tttatcgcct gtccggtaaa accgcaggca ggcatacagg
     4801 atgtagacaa gaaaaaccat gccaattgcg ctggtgatct ccaccagcgg cccgggcgcc
     4861 atctcttgat acaccacctg caggccaaag ggcagatcca cttgtttgat cgacgggcga
     4921 tccatgggcc aggaaaaacg gctgcgatca agccacagca ccacgccgct gatcgcgtag
     4981 taaatggaaa aggtgttgcg gacatagacc gagagctgcc ccgtatacag ccagatgaac
     5041 cacacgaaag ctatggccgc aaagaccgcc gacgtcatct gcaggcgctg ccagaattgg
     5101 ccgtccaggg gcgtggccgc actgtacaaa ccggccgtag ccgcggcgta aaacgcgaca
     5161 aacacgcaag caactgcaaa ggccaggtgc actttccgct cgcgaagccg cacccaggct
     5221 atggcgttgt gggcgccgat gcacaggagc gcgcccacca taagcatcgc tggtaaagat
     5281 gtaatcttca ttcgggtccg ttttcctcgg agccaggcct tcccgccgcc atacttccgg
     5341 gtacgggcac gtcatatacg aagccattat gcaccagacg tgtgaaaatg cgcatcttcc
     5401 gtcaaggaca gcccttatac gcctcttttt caggccgccg cccaataagc gctcttcgtg
     5461 gaattttgtg gcgcaccgca agcggagtat actccaggac agtgtacaca tggcctttca
     5521 ttccgtatcg tgaacctcat ggaaaggccg gagggaagtg aagaaatgac aagtgtagtg
     5581 atagcccttc tgtccggcgt cgggtttatc gccgcctacc acacctacgg gcgctatctg
     5641 gcgcgcaaga ttttctgcat agacccgaat gccgcggtcc cctcacaaga gctggaagat
     5701 ggcgtggatt acgtgcccac gcgcaaagaa gtcctgtttg gacatcattt tgtgtcgatt
     5761 gccggcacgg ggccgatcgt cggaccggcg attgcgatct tctggggctg gctgcctgcg
     5821 ctgttatggg tcctgctggg ctccatattc attggggcgg ttcatgattt cggcgctctg
     5881 gtggtgtcgc tccgctcgcg aggacaatcg atcggcgaca ttgccggccg gatgatttct
     5941 ccgcgggcgc gcgtcctctt cctggtcgtc ctgtttttcg tgttgatggt cgtgatcgcg
     6001 atcttcggac tcgtgatcgc cgtcatcttc tccatatatc cccaaagcgt gttgtcggtg
     6061 tgggtggcga tgccggtcgc ggtcggcgtc ggcttctgga tctaccggcg cggcgggcac
     6121 ctgctggcgc cgtcggtcct ggccctcgtg atcctgtacg ccgcagtcgc cattggcgtt
     6181 tactggtgcc cgattgacct gagcgcgctg ctccgaatgc cgttgtatgc gggcaatgag
     6241 gcgggcttct tcagcggcct cggttcggtc gtggtcatct ggaccggcgt cctgttggtc
     6301 tattgcttca tcgcgagcgt cctgcccgtc tggatgctgc tccagccccg ggattacatc
     6361 aattcgcacc agctccttgt ggccctcgtc ttgcttgctc tgggcctgct tgtagcgcgc
     6421 ccggacatgg ttgcgccggc catcaacggc aacgtgcccg cggatgcgcc tttgatgttc
     6481 ccgtttctgt tcatcacggt cgcgtgcggc gcggtctccg ggttccactg cctggtttca
     6541 tcgggcacct cgtcgaaaca gctccgcaac gaggcggacg ctcagttcgt gggctacggc
     6601 tcgatgctcg tggaaggcat gctggccgtc atcgtgatcc tggcctgcgg cgcggggctc
     6661 ggcctcggcg taatggagaa aggccagacc atcaccggcg ctgcggcgtg gtccgcctac
     6721 tacggcggcg aatggggcgc aatgagcgga ctggcgcgca acgtcggcgc gtttatcgaa
     6781 ggcgggggca acctgattgc cgccaccggc atcccccctc gcctggcggt cgggatcacg
     6841 gccgtcatga tcgcctgttt tgccgcaacg acgctcgaca ccgcaacccg tttacagcgg
     6901 tacatcgtgc aggagctggc ggacgtggtg cgggtgcccg cattgcacaa caagtatgcg
     6961 gctaccaccg tggccgttgt caccgggggc gcgttggccc tcctgccggg accaaacggc
     7021 cccggttcgg gtggactgat cctgtggccg ctgttcggcg ccatcaacca gctgcttgcg
     7081 gggttggcgt tcctggtcgt cgcgttctat ttgattcgcc acaaccgccc ggcctgggtg
     7141 ctggccgctc cgctggccat catggtggct ctgcctgcct gggcgatggg cattaacgtg
     7201 tccaattgga tcgcgaaaca caactggctc ctggccgcca tcgggagcgc agttctgctg
     7261 cttgagggct ggatggtcgt tgaagcagcg ctcatgtgga agagtgcccg gggcgtgctg
     7321 cccgaagcat tgccgccgct gaaaccggca accgcttctg cgctcgacga acgtgagcgc
     7381 tgcggctgaa agtccgtcaa cacacgagcg ttccgaaacg gagcaccaca gaaggagcct
     7441 ttatgaaaca ggccgccgtt ctcatctctt ttttgctcct gagtgccgtg gccggtgcgg
     7501 cagcggatgc gccccagcgc accctcgtgc tggatcccgg cgagggcaat ccacgcaatt
     7561 ccgagggcga ttttatccag ttgaacgacg gacggctcct cttcatctac acccatttca
     7621 cggggagtgc gagcgatcac ggcgcggcgt ttctggcgtc gcgtgtctcg accgacgccg
     7681 gagcaagctg gtcagccgag gatgccgtgg tgcttcccaa cgaaggcggc atgaacgtca
     7741 tgtcggtctc attgctgcgg ctccgtaccg gtgcgatcgc cctgttctac ctgcgcaaga
     7801 actccaccag cgattgccgg cccatgatgc gcctctcgaa cgacgaggcc gcgacctgga
     7861 gcgagcccgt gctctgcatc gaacccgtgg gatactttgt cgtgaacaat gatcgggtca
     7921 tccagctcga gagtggacgg cttgtgatcc ccgcagcgcg gcacagcctg cccggagaga
     7981 gcttcagcgg gcgcggccag gccatgtgct acctttcgga cgatgacggc gccacctggt
     8041 ttccaagcga atcgatcctg gacgcaccgg aagatagcaa gtccggcctg caggaacccg
     8101 ctgtggtggc gttgaacgac ggacgcctca tgatgctctg ccgcacggat cagggatgtc
     8161 agatgcgttc tttctccggc gacggcggca tcacctggtc cccgccggaa ttgacggaca
     8221 tcgccagccc ggtgtccccc gccaccatcg aacgcattcc atccacgggt gatttattga
     8281 tgctctggaa cgaccatcgc gcgatcgaac ccgccctggc gggcaagcgc acccccttcg
     8341 ccgcggccat ctcgcgcgac gaaggccaga cctgggagaa cgtgcgcctg cttgaaagtg
     8401 acccggacgg atggtactgc tacaccgcgc tcgaattcgt ggatgacgca gccctgctgg
     8461 gccattgcgt cggcgattcc accgtgggcc atctcaaccg gactcaggtg ctgcgcgtac
     8521 ccatcgcgtg gttttatgaa aactgaccca caacggcgct ccgcaccggg atttttctgt
     8581 atccgggctg aatctgcgta atccagcctc aaaagcaaag catccatccc gaatgggttg
     8641 acaaagcgga cgccgttcgc tcataacacg gccgccggag cgccgggaag aatctgatgg
     8701 gaaaggcggt tactgcatgc ccgtgaaatg gattgtcact gacctggacg gatgtgtgag
     8761 ccccgaggaa tccgtagcgt gggatctggg gccctttgtt gaatttgcac ggctttcgcg
     8821 cgcggcgtct gcgggtgaat ctacgctggc gccgctgacc ctctgcacag gccgccccca
     8881 gccctatgtc gaggtgttga tgaagatact cgacatccgc gcaccggcga tttgcgaaaa
     8941 cggggccgtg ttgtattcgc ttcatgacaa ttgggctcga ttcggaccgg gggtgacgtc
     9001 agagaagacc catcggttgc gggccgtgcg cgatttcatt gaaactgagt tgctgcatga
     9061 atatcctcag gcggtgatgc aattcggcaa agaagctcag ctttcggtgt tctcgcagga
     9121 ccccgcagtg ttgctcgcga tgcaaccgcg cgttgaacag ttcgtccggg aaaacggtcc
     9181 cgagctcatt atcaactgtt cgcattttta tctgaatctg tcgctggccg gagtggacaa
     9241 gggaagctcg cttcgcgcgc tgttcgacga gctgggcgtg cagccgcacg aggtggccgg
     9301 cattggcgac acagtaggcg atctgcccct gcgcgatgcg gtgggctttt tcgcctgtcc
     9361 gtcgaatgcg caggacgaaa tcaaagcggt cgcggactat atctcccctg aaccgaccgt
     9421 ttcgggtcta ttggacatcc tggcactgcc ggagatgcag cgtgggtgac aactgcgtgg
     9481 agggtgcagt catgtggatg cgtgcactgt gcattgtagg ttttggagcc atgactatgc
     9541 ccgcgtgggc gggcgcaggt gcctctatcc tgccgccgcc ccgccatggc ggagtctatg
     9601 tggtggcgca ccggggagcg catgacggcg ttccagaaaa ctcccttgcc gcctgccaga
     9661 aagccatcga actgggagcg gattttgtcg aagtggatgc gcggcagacg caagacggcc
     9721 atttcgtcaa catccacaac agcaccgtcg atgcgtatgc cgaaggcgca acaggcgcgg
     9781 tgcacgatct caccctggcc gagatacagg cgctggacat cggctcccgg gtcggtccgg
     9841 aatgggcggg aacgcgcatc cccaccgtcg atgaagtcct cgccctgtgc aaaggcaaga
     9901 taggcatcta catggatttc aaggacggat cgtgggagga catcctggcc ctggtgaaga
     9961 agcacggcat ggaacggcag gtgctctggt atgcgtcgaa cgagagactc aagaagatcc
    10021 gggaagcctg ccccaaatgc atcatcatgc ccgatccggg gccgggggcg aacctgaaac
    10081 cgcttctgga agatcttcgc ccgaaagtgg tcgcggccgt gtggaggcac tattccgagt
    10141 cgtttgtgaa gacctgccat gaagccggag ccatcgtgat cgtggacgaa catgacccgt
    10201 ccagctggga gccggccctg gcctggaaat ccgacggcat tcagaccgat caccccgaaa
    10261 aattgataga ggcgctgaaa aaacggggcc agactcagta gcggccttgg gtttcgacca
    10321 gttcgcgcat gcgatggatg cgggattcgt cggcgccggg tgggacctgg tcgcccgcgt
    10381 tggcgatgag gcgcgggctg gcatgccgca ggtccagcca gtcgaggacg gcccggtcga
    10441 agaccgccac gccaacgtcg ggcagccaca ggtgcggagg gacgcccccc gacaggatca
    10501 ggcccggccc ggcctcgtcg cggcagccgc tgggcgagag atcgccgggc tgtccgggtg
    10561 tcaccgcatc cgcgcaatct gcgccggtat cacggaacat gccgagtgcc ccgcgcaggc
    10621 gcccgtcgat atgtacggca acgcatttcc cggcccggtg cagccgcgcg atggcttcgg
    10681 tatagaatgc tccggaccat tcctcgaaaa aatggggcgg ctggatgtcg ctggagaagt
    10741 tgtcgcccat gagaataatt tcagcggggg attctgcgag catgtccacg cagtcgagga
    10801 tattggcgtt tacggcgtcg acgaactccc gcaccgcggc ctgcatctcg aaggtggcgt
    10861 agatcgcgcc ttccacaccc atccagtaat tgagcaggtg gcccatggcg ctgtaccccg
    10921 tgggcacgta aattacgccg gtatcaccga cagcacgagc ccacgcgtgg taccgttccc
    10981 aacgcggggt gtagctgcgg cgggacagcg cgtactgaag aatgccgagt tcccgctccg
    11041 atttcagcgg ccagccggta atgccccagg cgtaattttg cggttcccag cgccgcgtgc
    11101 gtgccagggt cccaagcggc gtatcaattg cccaggtgat cgcggtacca tcgggggact
    11161 ttgcagcgga agccgtgaca tcgccggggt agcaaacgtc ataaaacgcc gagagctgcg
    11221 gaagatagaa gccggccccc acttttctgt ggtacgcgat gagcgccgtc tccggctcag
    11281 gatgtgcagc gctcaaatcc cagggcatct cgtttcgatg atagaaccag tgggacaaat
    11341 cgagcatgaa ggggacggcg tcgggtatgc ccccccgata gacggtcagg atgcgctcgc
    11401 gcaaagtcat ggggccgatt ctcccggtgt ggccgcccca gcatacctga gtcgaaaccc
    11461 gctatccagt atcgccgcga tcgccacggg gttgattgcc ggtgggggtt cttgttagat
    11521 ttttcccgtt ctacacacgt gttctgtcga tcacacactg tttaaggcac ggagcagcca
    11581 tttgtactgt tccgctcgcg cagccaggcc gggagagaaa ccatgaaccg tacttcgtct
    11641 gccacaacgt accgcatcgt gtccggaacc ctgattctgc tcgccctgct ggcaggactg
    11701 agccccgaga cggcggcggc tccactccgg ctgcctgccg tgttcgacga ccatgcggtg
    11761 cttcagcgaa acgtctccgt gcccgtatgg ggatgggccg aacccggcga gacggtcgcc
    11821 gtgtccatcg cgggccagac tgcagccgca acggccggcg ccgacgggcg ctggcgggtc
    11881 gacctggccc cactggccgc cgggggaccc tttgaactca ccgtctccgc gccaagcggc
    11941 tccaggacgc tctccgacat tctggtcggc gaagtctggc tcgcatccgg acagtcgaac
    12001 atgcagtggt cgatcaaact ttccgacggc tgggacgccg gacaggcgca ctgggcgaac
    12061 gacaagatcc ggatcgcgat ggtgttccgt gaacacagcc ccgttccgct ggatgatctg
    12121 cgcggatgga ccccgtgggc cccgtgtaat gccgaaacgc tggacacctg ctacaacggg
    12181 gaaggcttct ccggagtcag ttactacttc gcgaaatacc tccaggccga gctgggcgtt
    12241 ccggtgggta tcatcaacac gagctggggt ggaacgcgaa tcgagccgtg gaccccgccc
    12301 gtgggtttcg agccgatccc cgcgctggcg gacatcacca cgcagattcg cctcaaccag
    12361 cccgaaaccg cggagtatca gcaggctctg aaagatgcga tcgagcaggt cgaggcctgg
    12421 cttccggccg cccggaccgc gctcgcgaat catacattcg caccggcact gccctccatt
    12481 gcctcggaat cgggcctgaa caacgaagga aggcccacga cgctctacaa cgccatggtg
    12541 gcgccgctcg tgccctatgc caaccggggc ttcatctggt accagggtga atccaaccgc
    12601 ggcgacggca tgctctaccg cgacaagatg gaagccctga tcaacggatg gcgcagcgtg
    12661 tggaacgaca atgccctcgc ctgctatttc gtgcaactgg caccgtttga ttatggcaaa
    12721 gcccccgaag ctttggcgga gatctgggaa gcgcaatcgg ccgcgacggc cattccgggc
    12781 accggcatgg cggtcatcaa cgatatcggc aacgtaaagg acatccatcc ccgcaacaag
    12841 gacgatgtgg gcaagcgcct ggcctggctc gcgctcaata aaacgtacgg aaaaagtgac
    12901 atcgtgtgtg aaggcccggt tttcgaccgg tttgagatcg tggacaacgc cttgcgcgtg
    12961 tatttcaaaa acgtgaaaac gctgagcacg cgcgatggcg ccgcaccgaa ctggttccag
    13021 atctgcggcg cggacggcgt ctttcatgag gcggcagcgg tcatcgacgg ggccagcgtg
    13081 gtgttgacgg cggcggatgt gaccacgccc gtggcggtgc gatttgcctg ggatcagtgc
    13141 gcagaaccca acctgaagaa cgaagccggc ctgccggcct cggccttccg cgcgggcaac
    13201 gtcccggccg gaggagcgga ataaacgtcc gtgaacgcga gctattgggg ccggtgggtg
    13261 tcatgaattc caccggcccc gctcttgtaa ggccgttttg attccgacag cagcaggcgc
    13321 agagccgtga acgccgcccg tagcgccacg ctccgctgtg gtatactgat tgtcaggagc
    13381 agtcatcagc gcgattaaaa ggaggtacgg agatgcgtt
//
LOCUS       DAOPBQ010000026        13393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4617_length_13393_cov_4.267656, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000026 DAOPBQ010000000
VERSION     DAOPBQ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 13393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13393
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4617_length_13393_cov_4.267656"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2054
                     /locus_tag="PKW60_01790"
     CDS             <1..2054
                     /locus_tag="PKW60_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPJ98192.1"
                     /translation="HAHNPVDWYPWGDEALDKARTENKPIFLSIGYSACHWCHVMERE
                     SFEDEAIAELLNRHYVSIKVDREERPDIDGIYMAATQIMTGGGGWPMSVFLTPDLKPF
                     YAGTYFPPRSRMGRPGFRTILESITAAWQTRHSEILESAERFTDYVRQNADTCTGSPN
                     APTPELIAVAAGDLLRSYDSNHGGFGPAPKFPPHGAINLLFRRYHATKDAQARDAALV
                     TLQKMANGGLYDHLGGGFHRYSVDERWLVPHFEKMLYDNAQLAPLYLAAYQITGDSFY
                     RRIAEETFDYVLREMTGPDGGFYSSEDADSEGEEGKFYLWRHDDIVELLGEEDAPLFN
                     EHYTVRPEGNFSSPEEYHAGLNILHVTSSLGDLAQRHGLSAAELEVRLGAMRRKLLAV
                     RSQRVRPGLDDKVLTSWNALMISAFAQGYQVLADENLLHAAEKAAAFLWSHLKDGERL
                     LRSHRNGESRFDAYLDDHAFLAVALLDLYEAAFDVRWLEAAREIADIMLAKFWDREGG
                     GFFFTSPGHTHLIARDKPVFDGAEPSGNAMAAYALLRLATFSGEAEYGDKAERLLCIN
                     HAGLESMPRAQLSMLCAVDFYLHAPKEIAIAGKSGSPDVLVLLQALHRHYIPNKVVAF
                     IDPDAPDAQRARDATPLLDAKMPVDGKAAAYVCKDFACRQPVTSPEALLEELEVDREQ
                     E"
     gene            2061..3836
                     /locus_tag="PKW60_01795"
     CDS             2061..3836
                     /locus_tag="PKW60_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPJ98193.1"
                     /translation="MAYSKVSRFILLGCVLILALPSAAQFGALQQPSEPPEATVAAYQ
                     DATGALPGAQTRLAVVIEIPSGWHINAHEPLDEFLIPTQVVLEPPDGFAVDAVVYPEP
                     VLATFSFSQDQMAVYEHSAAVGLVVAVAEHVAPGEYALPGTLRYQACNDTICAPPRTL
                     PFVAQLTVVAAGTSVTPQNSEIFAPIDFTKTAAPESAPGEAAQAPVETVSEDWEALAD
                     AFVVAGRNSGYLGSDTFIEWIRGVESGALDSSLNAFAGKSAFAIIVLTLLGGLALNLT
                     PCVLPLIPINLAIIGAGAQAGSRRRGFALGGVYGLAIALTYGILGIAAVLTGSSFGSI
                     NASPWFNLAIAVVFVVLALAMFDLINIDFTRYQSRVAIDKSRKGTFYLAFGMGAVSAL
                     LAGACVAPVVIFVILLARDLYQASPIALALPFVLGAGMALPWPFLGAGMSFLPKPGGW
                     MVKVKYAFGVFILLFAAYYGYLSYSIFSDRLGVDRMEVASSAEELDEDGWITSLADGL
                     ARAKAENKPVLVDFWATWCKNCLTMNQTTFKDPRVQESLADYVKVKYEAPQPDAPPAR
                     DVMERFGVGGLGLPVYVILEPTASE"
     gene            complement(3857..5125)
                     /locus_tag="PKW60_01800"
     CDS             complement(3857..5125)
                     /locus_tag="PKW60_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ98194.1"
                     /translation="MNNKIMAAAMALLAVLLALQTYRLHLAQTEGAESGMTGPAPPAS
                     ATNREELARAGLLLEETFQPGRGDASTDPLADYLVEALASFLDQDPLHDGTPDSAPRR
                     GNRAETAASGAGRASGRPADADGRVDGKASRAQRRRNHGETGSEETRAAAADPRRSRQ
                     LYNRARQLIERGSYAEAAAMLRDSLAADGSNAMSYRTLGALYRQLGMTAEEMQVYADW
                     SAARPGDPLAHYYLANAYLRTGADAAVLGELDQFRALSADHPGSYAMAASIYRRLGLP
                     GEEGAMLATWVEQAPQSFDARQALAQYYQRSGQHDAALAEYQAAVSLIPDNVQARANL
                     ASAYSRLGLYADAQSEYAAALALRPADPGLHFQMAEMYRRANDLPAALASYQQVIDLR
                     PESPQAQRAARMMTRIERQLSTAAAPKLLR"
     gene            complement(5313..5657)
                     /locus_tag="PKW60_01805"
     CDS             complement(5313..5657)
                     /locus_tag="PKW60_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HPJ98195.1"
                     /translation="MTHVVTEACIKCKYTDCVTVCPVDCFHEGANMLAIDPVECIDCG
                     ACVDECPVHAIYPEEDLPEEYQDFLQLNLEYSAKWPVIAEGHAPLDTAEEYKDVKDKR
                     DFFDPSPAPRDE"
     gene            complement(5818..6834)
                     /locus_tag="PKW60_01810"
     CDS             complement(5818..6834)
                     /locus_tag="PKW60_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004059578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HPJ98196.1"
                     /translation="MALTNRQIYLAARPKGLPRESDFALVASEVPALQEGQFLVRINY
                     VSVDPYMRGLMNEGPSYMNPFNVGDLILGGAAGTILESRHPEYAVGETVHGLWGWQDY
                     AVSDGSNVYRFDTSLAPLSTSLGILGMPGLTAYFGLLDIGRPRPGETVFVSGAAGAVG
                     SVVGQLAKIQGCRVAGSAGSAEKVACLKSIGFDAAFNYKDLTDYAAALREACPSGIDV
                     YFDNVGGALTDAVIPQVNPEARIVVCGQIDQYNALAPAQGPRLLWHLITKRARAEGFL
                     VFQFAARYEEAQRALAQWIAEGRLTWRETIVDGLENTPRAFIGLFKGENLGKQLVRVA
                     EPGS"
     gene            complement(6928..7683)
                     /locus_tag="PKW60_01815"
     CDS             complement(6928..7683)
                     /locus_tag="PKW60_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98197.1"
                     /translation="MPRPLSAIILAVWLALVPGCGPGREKIVEIPAIDLLLASEVMGR
                     CVEAYENCASYRDSGVMTRTFRTANETQEESQTFSTAFQRPDRFRFQCQEGEHRYIVY
                     ADAGTIRAWWGHQEGAQPMPSLDDAIGRAVGPSNGASHTIPRLLMPDRVHGRSLAELH
                     GLSRLSDALLEDVPCYRINGSLRTGALYTVWIDRATFLVRRIDAEHIAPDYSTTDCTV
                     YFPELNPELADQELLLNAPASKPSGRALTARGE"
     gene            complement(7835..9070)
                     /locus_tag="PKW60_01820"
     CDS             complement(7835..9070)
                     /locus_tag="PKW60_01820"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPJ98198.1"
                     /translation="MFRVMSCVLAAVLGGSLAAKAAESADAPAESPFSIQLDIAHEGY
                     DETYCWFQPRAAAIPRKTGEEPPIVIMTLQKHLESSDFYSGLYTMRSDDLGQTWSPPQ
                     EQPALGWRSLPDDITLGICDFVPGWHAPSGRLLAIGHTVRYRNRVLMASPQPRELAYA
                     VYDPEMDQWDPWRMLEFPDQEKFWSAGAGCAQWVIKDDGTLLVPQYFCPPEMRGEGAR
                     ARSASTVVHCSFDGTQLTYLEHGDELTLDVPRGCDEPSLIQFQGRYYLTIRNDEKGYV
                     TAGDDGLHFAPLKPWTFDDGAELGSYNTQQHWLAHGGRLFLVYTRRGANNDHIFRHRA
                     PLFIAQVDPDHLCVMRATEQVLIPERGATMGNFGAGPITQGESWVTVGEGMFFPEVYK
                     KGGATGAVFVARVRWQPDN"
     gene            9345..9791
                     /locus_tag="PKW60_01825"
     CDS             9345..9791
                     /locus_tag="PKW60_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98199.1"
                     /translation="MDLSRFKWPIIILVVIGVFWLFSSAGINFMHKKFTSVPPDRDAK
                     VDQANEKGLSALGGLLIKTFRYEKGFEVLNDASRLYPQGANFWYNQYRMAKCLEKMDR
                     ISEALKLLDNLYVNDIHALDNRVPPRDVLKLRIDKLQEVHGLGEIQ"
     gene            9799..10530
                     /gene="rlmB"
                     /locus_tag="PKW60_01830"
     CDS             9799..10530
                     /gene="rlmB"
                     /locus_tag="PKW60_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPJ98200.1"
                     /translation="MRQRIAGRIPVLECLRAGKRAPRRLFVLPSAQGMDGILDAAAGV
                     PIETRPRAELDQLADGTVHQGVVLEADPLPVLCEKDWLAQSLPADALAVVLDGIEDPH
                     NFGAIVRSASALGAHGVFFAKRHAAPLSAAALKSAAGAMEYIDLVEVTNITRALGTLK
                     KAGFWVAGFDADAPQTLWQADLSGRLAVVIGSEGAGMRRLVQETCDFLVRIPLTGAIT
                     SLNASVSAAIALAECRRRRAGDSGP"
     gene            10696..12177
                     /locus_tag="PKW60_01835"
     CDS             10696..12177
                     /locus_tag="PKW60_01835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPJ98201.1"
                     /translation="MFQWIKPKSEFTEADTEKALSRLVYDGMFGQTMMVLSTGPFLVA
                     FALILGASNTVIGMLAAVGPLAQMLQIPAIFVVEGIRMRKAITVVTAIISRSALFAIA
                     MLPWFAPKDYRIPLFLLFLACHYGIGAFAGCAFSSWLRDLIPGDRMGRFFARRLTFST
                     GTGAVLSLLGGVAIDQYREHFADPAGAYTIVFSLAGLAGMVSVVFMGSTPEPALPEAE
                     HVPLWELLVRPFRDTNFRNLLTFLACWGAAVNFAAPFFTVYLLQRIGLNMTWVLALSV
                     VSQITNVLFFGLWGRLSEQFTNKSVLVFSVPLFFISFLFWPFTTLPERHFLSIPLLIA
                     IHVIAGMSTAGVALCANNLAMKTAPYGKGASYLAVNALVSGIAATVSPVLAGFAADWF
                     DPFELQLTLSFTDWTREYSAWELPTIDLKGLDFVFVIAFLIGVYCFHRLLAVREEGEV
                     TEQVLRQALFGEVGRMVRQISSVAGMRQIVSFPFAYLIGLKRK"
     gene            12530..>13393
                     /locus_tag="PKW60_01840"
     CDS             12530..>13393
                     /locus_tag="PKW60_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HPJ98202.1"
                     /translation="MVTKTPMHHSELQPNSEDGSQSQDSLQQTVPLLRFPPWPYLTCR
                     EATLMCVALMVLELYAFAVLCLPIAAGGLLCDIPLRQNHLLWNPAIHIAANVWAVIPL
                     FFWAMGRSGRPFRETFGIYPVRWVVLWAATFLGAVTATLTGHLGGIFAQGFPSSSFPF
                     EASEVFRASPNMSCFALVVVAPLTEELLFRGVILQGLLRRRSIGRSCVVSSLLFALIH
                     VNPFQFVPLFLGGLLLAAVQLKTGSLWPSICMHAGFNIPPAAAIMAQARLAATQEAGP
                     PSFPWFHVFAGV"
BASE COUNT         2336 a         4168 c         4156 g         2733 t
ORIGIN      
        1 aacatgccca taacccggtc gactggtatc cctggggtga tgaagcactg gacaaggccc
       61 gaaccgagaa caagcccatc tttctttcga ttgggtattc cgcctgccac tggtgccacg
      121 tcatggagcg ggaatcgttc gaggacgaag cgatcgccga attgctcaac cggcactatg
      181 tgtcgatcaa ggtggatcgc gaggagcggc cggacattga cggcatctac atggccgcca
      241 cacagatcat gacgggtggc gggggctggc ccatgagtgt gtttcttacc cccgacctga
      301 agcccttcta cgcgggcacc tactttcctc cacgctcccg gatgggccgg cccggtttcc
      361 ggaccatact cgaatccatc accgccgcgt ggcagacccg gcacagtgag atcctcgaaa
      421 gtgcggagag gttcacggat tacgtgcgtc agaacgccga cacctgcacc ggttcgccca
      481 acgcgcccac gccggaactc attgcggtgg ccgccggtga tctgttgcgc tcgtacgact
      541 caaatcatgg tggattcggc ccggccccga aatttccgcc gcacggcgcc atcaacctgc
      601 tctttcggcg gtatcacgcg acaaaagacg cccaggcccg ggacgccgcc ctggtgaccc
      661 tgcaaaaaat ggccaacggg gggctgtacg atcatctggg cggcggattt caccgctatt
      721 cggtcgatga acgctggctt gtaccgcatt tcgagaagat gctctacgac aatgcccagc
      781 ttgcgccgct gtatctcgcc gcgtatcaga tcacgggcga ttcgttctac cgccgcatcg
      841 ccgaggagac gtttgattat gtcctccggg agatgacggg ccccgatgga gggttctatt
      901 cgtcggaaga cgccgacagc gaaggggagg aggggaaatt ctatctgtgg cgccacgacg
      961 acattgtcga gctcctgggc gaagaggatg caccgctgtt caatgagcac tatacggtgc
     1021 gcccggaagg taatttttcg tcgccggagg aataccacgc tggtctgaac attctgcatg
     1081 tcaccagcag cctgggcgat ctcgcgcagc ggcacggcct gtccgccgca gaactcgagg
     1141 tccggcttgg agccatgcgc cggaaactgc ttgccgtgcg ttcgcaacgg gtgcggccgg
     1201 ggttggacga caaggtgctg acttcgtgga acgccctgat gatctcggct tttgcccagg
     1261 gataccaggt gctggcggac gaaaatctgc tccacgccgc ggagaaagcc gccgcatttc
     1321 tatggtcgca tctgaaagac ggcgagcgcc tgctgcgcag ccatcgtaac ggcgaaagcc
     1381 gtttcgatgc ctatctcgac gatcacgcct ttctcgccgt ggccctgctc gacctgtacg
     1441 aggccgcgtt tgatgtgcgg tggctcgagg ccgcgcgcga gatcgcggat atcatgctgg
     1501 caaaattctg ggatcgggaa ggcgggggat tcttctttac ctcgccgggc catacccatc
     1561 tcatcgcacg ggacaagccg gtgttcgatg gcgcggaacc gtcgggcaac gccatggccg
     1621 cgtacgccct gttgcggctt gcgacgttct ccggcgaggc cgaatacggg gacaaggcgg
     1681 aacgtctcct gtgcatcaac cacgccgggc tcgaatccat gccgcgtgcg cagctcagca
     1741 tgctgtgtgc ggtggacttt tatctccatg ccccgaaaga gatcgctatt gctggcaaga
     1801 gcggctcgcc cgacgtgctc gtcctgcttc aggcgttgca tcgccactac attccgaata
     1861 aggtagtggc attcatcgat ccggatgcgc cggacgccca gagagcacgg gacgccacac
     1921 cgctgctcga cgcgaaaatg ccggttgatg ggaaagccgc cgcgtatgtg tgcaaagatt
     1981 ttgcctgccg ccagccggtc acgtcgcccg aggcgcttct ggaggagttg gaggtagatc
     2041 gcgaacagga gtaagaatcc atggcgtatt cgaaggtatc tcgttttatc ctcctcgggt
     2101 gtgttttgat cctcgccctg ccgtcggcag cccagtttgg cgcgcttcag cagcccagcg
     2161 agccgcccga ggccacggtc gccgcatacc aggacgccac cggcgcgctg ccgggcgcac
     2221 agacacgcct cgcggtggtg atcgagatcc ccagcggctg gcatatcaac gcccatgaac
     2281 cgctcgacga attcctcatt cccactcagg tcgtgctcga gccgccggac gggtttgccg
     2341 ttgacgccgt ggtctatccc gaacctgttc tggcgacgtt cagcttttcg caggaccaga
     2401 tggccgtcta tgaacattca gccgcggtcg ggcttgtcgt ggccgtcgcc gaacatgttg
     2461 cgcccggcga gtatgcactg cccggcacgc tgcggtacca ggcctgcaac gacacaatct
     2521 gcgcgccacc caggaccctg cccttcgtgg cgcagctcac cgtggtcgca gccggcacct
     2581 cggttactcc ccagaattcg gagatttttg cgcccatcga cttcacgaaa actgccgcgc
     2641 cggagtccgc gcccggcgag gcggcgcaag cgcccgttga aacggtttcg gaagattggg
     2701 aagcgctcgc agatgccttt gtcgtggccg ggcgcaactc cgggtacctg ggcagcgaca
     2761 cgttcatcga gtggattcgc ggcgtcgaat cgggcgcgct ggacagcagc ctcaacgcgt
     2821 ttgccggcaa gagcgcgttt gcgattatcg ttttaacgct gctgggcggc ctcgctctta
     2881 accttacgcc ctgcgtgctc ccgctgattc ccatcaacct ggcgatcatc ggcgcgggcg
     2941 cccaggcggg ttcgcggcgg cgcgggttcg ccctgggtgg cgtctatggc ctcgccatcg
     3001 cgctgaccta cggtatcctg ggcatcgctg ccgtgctgac cggctcgtcg tttgggagca
     3061 tcaacgcatc gccgtggttc aatctggcta ttgcggtggt gtttgttgtg ctggccctgg
     3121 ccatgtttga cctcatcaac atcgatttta cgcgctatca gagcagggtc gcgatcgaca
     3181 aatcccgcaa aggaacgttt tacctcgcgt ttggcatggg ggcggtctcg gcgctgcttg
     3241 cgggtgcctg cgtcgcgccg gtggtgatct tcgttatcct tctggcgcgc gacctctacc
     3301 aggcgagtcc gatcgccctg gcactgccgt tcgtgctcgg cgcaggcatg gcgctgccct
     3361 ggccgttcct gggcgcgggc atgtcgttcc tgcccaaacc cggcggctgg atggtcaaag
     3421 taaagtacgc gttcggcgtg ttcattttgc tctttgccgc gtattacggc tatctcagtt
     3481 actcgatttt cagcgaccgc ttgggcgttg acaggatgga ggttgcctcg agcgcggaag
     3541 aactcgacga agacggctgg atcacatctc tggccgacgg gcttgcccgc gctaaggccg
     3601 agaacaagcc agtgctggtt gatttctggg ccacctggtg caagaactgc ctcacgatga
     3661 accagaccac cttcaaggac ccgcgggtac aagaatccct cgcggactac gtcaaggtga
     3721 agtatgaggc gccgcaacct gatgcgcccc ccgcccgtga cgttatggaa cgattcggcg
     3781 tcggcgggct cggccttccc gtctacgtca tccttgaacc cactgccagc gagtgaccgt
     3841 cggggggatg cgggcgctat cggagaagtt tcggcgccgc agccgtgctc aactgccgct
     3901 cgatgcgtgt catcatgcgg gcggcacgct gggcctgcgg cgattccggc cgcaagtcga
     3961 tcacctgctg gtatgaggcc agcgctgccg gcagatcatt cgcgcggcgg tacatctcgg
     4021 ccatctggaa atgaagcccg gggtctgccg gccggagagc cagggcggcg gcatactcgg
     4081 attgcgcgtc ggcatacagg cccagccgcg aataggccga ggccagattc gcgcgggcct
     4141 gcacgttgtc gggaatcagc gagacggcgg cctggtattc ggccagggcg gcgtcgtgct
     4201 ggccggagcg ctggtaatat tgcgccagcg cctggcgggc atcaaacgat tgaggcgcct
     4261 gttcgaccca cgttgcgagc atcgcccctt cctcgccggg caggcccagg cggcggtaaa
     4321 tcgaggcagc catggcatac gagccggggt ggtccgcgct caaggcccgg aactggtcca
     4381 gctcgcccag gacggcggca tcggcccccg tgcgcaaata ggcgtttgcc aggtagtaat
     4441 gcgcgagcgg atcgcccggg cgcgcggccg accagtccgc atagacctgc atttcctcgg
     4501 cggtcatgcc aagttggcgg tataacgctc ccagcgtgcg gtaggacatc gcgttggacc
     4561 cgtcggcagc caggctgtct cgaagcatgg cggcggcttc cgcgtaactt ccgcgttcaa
     4621 tgagctggcg cgcccggttg tacagctgtc tcgatcgtct gggatcggcc gctgcggcgc
     4681 gggtttcctc acttccggtt tctccgtgat tccgccgccg ttgtgcgcgc gaggccttgc
     4741 cgtccacgcg tccatcggcg tctgcgggac ggccgctcgc gcgaccggcg ccggatgcgg
     4801 ccgtttcagc tcggttcccc cggcgcggcg cagaatccgg cgtgccgtca tgcagcgggt
     4861 cctgatcaag aaaagaggcc agtgcttcga cgagatagtc ggccagggga tcggtcgacg
     4921 catcgcctct gccgggttgg aaagtctctt cgagcaggag tcccgcgcgc gcaagctcct
     4981 cccggttcgt ggccgaagcc gggggcgcag ggccggtcat cccggactcg gccccctccg
     5041 tctgcgccag atggaggcgg taggtctgga gggccagcag aaccgccaga agcgccatgg
     5101 cagcggccat tattttgttg ttcatggttg ggactccacc cgggaattcc ccggggacgc
     5161 cggcaagaaa tcctgtcaga ccgatcggac gcatgggaag tatagctgct gtgcggatta
     5221 tatcatcaag agccgggcgc tctgcggtgg gaaaaatgaa aacagcctgc cgggacacaa
     5281 tggtcgcggc gggcgggggt acgttcggga gcctactcgt cgcgtggcgc cggcgagggg
     5341 tcaaagaagt cgcgcttgtc cttcacgtcc ttatactcct cggcggtgtc gagcggcgca
     5401 tgcccttccg cgatgacggg ccatttcgct gagtattcaa gattgagctg tagaaaatcc
     5461 tggtactctt ccggcaggtc ttcttccgga tagatcgcgt ggacggggca ttcgtcgacg
     5521 catgcaccgc aatcgatgca ttccacaggg tcgatcgcca gcatgttggc gccctcgtga
     5581 aagcagtcaa ccgggcacac ggtcacgcag tcggtatatt tgcatttgat gcacgcttcg
     5641 gtaacaacat gggtcataaa tatgcctcct gcaagcggcc aaaccggcat acttggagtg
     5701 agttgaactg cgcttcgaac aagccgaact ggcgggcgat tgcgcgccat tgaagtatca
     5761 tgtttagaca caccgtgtca atcagaatat tcccggcgcg cactttccgc ggcagcgtta
     5821 cgaaccgggt tcagcgacgc gaaccagctg ttttccgagg ttttcgccct tgaacaagcc
     5881 gataaacgcg cgcggcgtgt tttccaagcc atccacgatg gtttcccgcc aggtcagacg
     5941 gccttcggct atccactggg ccagagcgcg ctgagcctcc tcgtagcgcg cggcgaactg
     6001 aaatacgaga aacccctcgg cgcgtgcgcg ttttgtgatc aaatgccaca agaggcgcgg
     6061 tccctgggcc ggagcaagcg cattgtactg atcgatctgg ccgcaaacca caatgcgtgc
     6121 ctccggattg acctgcggaa tcacggcatc ggttaatgcg cctccaacat tgtcaaaata
     6181 cacgtcaatg ccgctgggac atgcttcccg caatgcagcc gcgtagtcag ttaggtcttt
     6241 gtaattgaac gcggcatcaa agccgatcga tttgaggcag gccactttct cggcggaacc
     6301 cgccgagccg gccacacggc aaccctggat ctttgccagc tggcccacta ccgaacccac
     6361 cgcaccggct gcgcctgata caaatacagt ttcgccgggc cgcggccggc cgatatccag
     6421 caggccgaaa tacgcggtca ggcccggcat gcccaggatg cccagggacg tggataatgg
     6481 cgcgagcgac gtgtcaaacc ggtatacgtt gctgccatcc gacaccgcgt agtcctgcca
     6541 gccccagagt ccgtggaccg tctcgccaac ggcatattcc gggtggcggg attcgaggat
     6601 cgtgcctgcc gcgcccccca ggatcaggtc gcccacgttg aacgggttca tgtaggatgg
     6661 cccttcattc atgagccccc gcatataggg atcgacggag acgtagttga tccggaccag
     6721 aaactgacct tcctgcaacg cggggacttc gctggcgacc agcgcgaaat cagattctct
     6781 ggggagtcct ttcggccgcg ccgccaagta gatctgccgg tttgtgagtg ccatgacctg
     6841 ttcctttccg ctgttttacc gcgagacctg atggacggcc tgtggttaag cgcaacaact
     6901 gtggcggccc gggcatttcc gcgccggtta ttctccccgc gcggtcaggg ccctgcccga
     6961 aggtttggac gccggggcat tcaaaagcag ctcctggtcg gccagttccg ggttcaactc
     7021 cgggaaatac acggtacaat cggtcgtgct gtagtcggga gcgatatgct ccgcatcgat
     7081 ccggcgcacc agaaacgtcg ctcgatcaat ccagacggtg tacaacgcgc cggtacggag
     7141 cgagccgttg atccggtagc acggcacatc ctccagcagg gcatcggaca ggcggctcag
     7201 tccgtgcagt tctgccaggc tccgtccgtg gacccgatcc ggcatcagca gccgcgggat
     7261 ggtgtgggat gcgccattgg acggccccac ggcgcgcccg atggcatcgt cgagcgacgg
     7321 catcggctgg gcgccctcct ggtgccccca ccacgcgcga attgtgcctg catcggcgta
     7381 aacgatatac cggtgctcgc cttcctggca ctggaaccgg aaacggtcag gacgctggaa
     7441 ggcggtgctg aacgtctgcg attcctcctg cgtctcattg gccgttcgga acgtgcgcgt
     7501 catgaccccg gaatcgcggt atgaggcgca attttcgtag gcctcgacgc aacggcccat
     7561 cacctccgag gccagcagca gatcgatggc gggaatctcc acgatctttt cgcggcctgg
     7621 cccgcacccg ggcacgagcg caagccagac ggccaggatt atggcggaca aagggcgtgg
     7681 catgcgggca accgtgctcc catctatgat ggcgtgtttt tatgaatcat aacacgcttt
     7741 ctgcagccga gctacggggc aggacccctt gaggcaggcg tggctatggc ctgcagcgct
     7801 caggacgctg ttaatgagga tccgcgccgg cgcctcagtt atccggctgc cagcggaccc
     7861 gagcaacgaa aaccgcgcct gtcgcaccgc ctttcttgta gacctcgggg aagaacatgc
     7921 cttcgccgac ggtgacccag ctctcgccct gtgtaatggg gccggcgccg aaattgccca
     7981 tggtggcgcc gcgttccggg atcagcacct gttcggtggc ccgcatgaca cacaggtgat
     8041 ccggatccac ttgtgcgatg aacagcggcg cgcggtggcg gaagatgtgg tcgttatttg
     8101 cgccgcggcg cgtatagacc aggaacagcc ggccgccgtg cgccagccag tgctgttgcg
     8161 tgttgtaact gcccagttcc gcgccgtcgt caaatgtcca cggtttaagc ggcgcgaaat
     8221 ggagcccgtc gtcacccgcg gtcacgtaac ctttttcatc attgcggatc gtcagatagt
     8281 agcgtccctg gaactgaatc agggacggct catcgcatcc tcggggcaca tccagcgtga
     8341 gttcgtcgcc gtgttccagg taggtcagct gcgtcccgtc aaaagagcaa tgcacgacgg
     8401 tggatgccga gcgcgcccgt gcgccttccc cgcgcatttc gggcggacag aagtattgcg
     8461 gcacgagcag cgtgccatcg tctttgatca cccattgcgc gcatccggcg cccgcgctcc
     8521 agaatttctc ctggtcagga aactcgagca tgcgccaggg gtcccattgg tccatctccg
     8581 ggtcgtacac ggcgtaggcc agctcacgcg gctggggtga cgccatcagt acccggttcc
     8641 ggtagcgcac cgtatggccg atagccagca gtctcccgct gggtgcatgc catcccggca
     8701 cgaaatcgca gatccccagc gtgatgtcgt ccggaagcga acgccatccc agcgcaggct
     8761 gttcctgtgg tggcgaccag gtctgcccga ggtcgtccga gcgcatggtg tagaggcccg
     8821 aatagaaatc ggagctttcg agatgtttct gcagggtcat gatgacgatc ggcggttctt
     8881 cgcccgtttt acgcgggatg gccgcggccc gcggctgaaa ccagcaatac gtttcatcgt
     8941 acccttcgtg ggcaatgtcg agctggatag aaaacggtga ttccgccggc gcgtcagcgc
     9001 tctctgctgc cttcgcggca aggcttcccc ccagtacggc ggccagtacg caactcatca
     9061 ctcgaaacat gcgccttctc ctcgtttcat gcgccgtctc atgccctcag atatgagtcc
     9121 gcgccgatgg gcgtcagtat gatgaggtgg tttctgcaaa tcaattgccg ccgggcgcca
     9181 aacacgagag ccgcagcgat tctggggcgc cgatgctcac tatccgtggg ataaagcggc
     9241 acgtgctccg cctgttcatg ggatgtgcta agataacatc ggcttaggcg ggccgaagcc
     9301 gttcatcagt caacgaagca ggcgcgtcgc gccgggaggg catcatggat ctcagccgct
     9361 tcaaatggcc tatcatcatc ctcgttgtca tcggtgtgtt ctggttgttc agcagcgctg
     9421 gcatcaattt catgcataag aaattcacgt cggtcccgcc cgatcgggac gccaaagtgg
     9481 accaggccaa tgaaaaaggc ctgtcggccc tgggcggctt actcatcaag acatttcgct
     9541 acgagaaagg gttcgaagtc ctgaatgatg cgtccaggct ctatcctcag ggggccaatt
     9601 tttggtacaa ccagtaccgc atggccaaat gcctcgaaaa aatggatcgt atcagcgagg
     9661 ccctcaagct tttggataat ctctacgtca acgacattca tgccctggat aatcgcgtgc
     9721 cgccgcggga cgtgctgaag ctgcgcatcg acaagcttca ggaagttcac gggctgggcg
     9781 agattcaata gagccatcgt gagacagcgc atcgcaggac gtatccccgt gctggaatgc
     9841 ctgcgcgcgg gcaaacgcgc cccgcgcagg ctgtttgtgc tgccctcggc gcaaggcatg
     9901 gacggcatcc tcgatgccgc tgccggcgtt cccatcgaaa cgcggccccg cgctgaattg
     9961 gaccagctgg cggacggcac ggttcaccag ggcgtggtgc ttgaagccga cccgctgcct
    10021 gtcctgtgcg aaaaagactg gctggcgcag tccctgccgg ccgatgcgct cgcggttgtg
    10081 ctggacggga tcgaagaccc gcataatttc ggcgcgatcg tgcgctcggc ctctgcgctg
    10141 ggcgctcacg gcgtgttttt cgcgaaacgt catgccgctc cgctctccgc ggcagcgctc
    10201 aagagcgccg caggcgcgat ggaatatatc gatctggtcg aagtcaccaa catcacgcgg
    10261 gcgctgggca cgctgaaaaa agccgggttc tgggtggcgg ggtttgatgc ggatgcgccc
    10321 cagacgctct ggcaggcgga tctgagcggc cggctcgccg ttgtcatcgg cagcgaaggt
    10381 gcggggatgc gtcgccttgt ccaggaaacc tgcgatttcc tcgtgcgcat ccccctgacc
    10441 ggcgccatta cctcgctcaa cgcctcggtc agcgccgcca tcgccctggc tgaatgccgc
    10501 cggaggcgcg ccggggactc cggcccgtaa tcgacagccc cataggcccc gtgcctccct
    10561 gcatccgccg tgccgcggat catccatctt gccaatggcc ggtcaagcgc gtaagatccc
    10621 gtacactacc aggtttcggc ctgattccgg ttaatgccag ttgaactcca aaatccgggt
    10681 acgatagcag cgcacatgtt ccaatggatt aaacccaaat ctgaatttac cgaagcggac
    10741 accgagaagg ctctgtcgcg gctggtctac gacggcatgt tcggccagac gatgatggtc
    10801 ctgtccacgg gcccgtttct ggttgccttt gcgctcattc tgggcgccag caacactgtg
    10861 atcggcatgc tggcggcagt ggggcctctg gcgcaaatgc tgcagatccc cgccatcttt
    10921 gtggttgaag gcatccggat gcgcaaggcc atcaccgtgg tcacggccat catcagccgc
    10981 agcgccctgt ttgccattgc gatgctgccg tggtttgctc ccaaagacta ccgcattccc
    11041 ctgtttttgc tgttcctggc ctgtcattac gggatcggcg cctttgccgg atgcgcgttc
    11101 agttcctggt tgcgcgacct gatccccggc gatcgcatgg gccggttttt cgcgcgccgc
    11161 ctgacgttca gcacgggcac cggcgcggta ctgagcctgc tgggcggcgt ggccatcgac
    11221 cagtaccgcg aacacttcgc cgatccggcg ggcgcctaca ccatcgtctt ttccctggcg
    11281 ggactcgccg ggatggtgtc ggtggttttc atgggcagca cgcccgaacc cgcgctgccc
    11341 gaagccgaac atgtgccgct ctgggaattg ctggtgcggc cgttccgcga cacgaatttc
    11401 cggaacctgc tcacgttttt ggcgtgctgg ggcgccgcgg tcaacttcgc tgcgccgttc
    11461 tttaccgtgt acctcctgca gcgcatcggg ctcaacatga cctgggtgct cgcgctttcg
    11521 gtggtctcgc aaatcaccaa cgtactgttt ttcggtttat ggggccggct ttccgagcaa
    11581 ttcaccaaca agtctgtgct ggtgttttcg gtcccgctgt ttttcatcag ctttttgttc
    11641 tggccattca cgaccctgcc cgaacgccac tttctgagca tccccctctt gatcgcaatc
    11701 cacgtgattg cggggatgtc cacggcgggc gtcgcgctct gtgcgaacaa cctcgccatg
    11761 aaaaccgcgc cgtatggcaa aggcgcttcg tatctcgcgg tcaacgcgct cgtgtccggt
    11821 atcgccgcca cggtctcgcc ggtactggcc gggtttgccg cggactggtt tgaccccttt
    11881 gaattgcagc tcaccctgtc gtttaccgat tggacgcggg agtacagcgc gtgggaattg
    11941 ccgaccattg atctcaaggg tctggatttt gtcttcgtga tcgcctttct tattggcgtg
    12001 tactgtttcc accgtctcct cgccgtgcgt gaagaaggcg aagtgaccga gcaggtgctc
    12061 cgccaggccc ttttcggcga ggtaggccgc atggtccggc agatctcgtc ggtcgcgggg
    12121 atgcgtcaaa tcgtgtcgtt cccgtttgcc tatctgatag ggcttaaacg aaaataatcc
    12181 ggcaggaagc ctttcgcgat gccgtctacc gggcatgtga gcttggcatt ttcgcccgag
    12241 tgcggtatcg ttgcggccga tcgaggcgga cgggccgcat cggcgcaagg agggtcgccg
    12301 catggtgcgc atgaccaagg tgcggggatt cctgggtgca gtggtagcaa ttgtcgttct
    12361 ggtggtcttt atcgcgattg tcgcgcgcgt gcttgggtgg gacattcccc tgctcaacta
    12421 aaattgccga accagccggc ctggcgccac acgcctgcgc agcggagcag ccccgcctcc
    12481 gtgacttgcg ttggctccat gcagccgcat gattcgccgg cagcaaagaa tggtcacaaa
    12541 aacgcccatg catcacagcg agttgcagcc gaattccgag gacggctcgc agagccagga
    12601 ttcgctccag cagaccgtgc cactcctccg attcccgccc tggccttacc tcacctgccg
    12661 ggaagccacc ctgatgtgtg tagccctcat ggtgctggag ctttacgctt tcgcggtcct
    12721 ctgcctgccc attgccgccg gcggactgct ttgcgatatc ccgctgaggc agaatcacct
    12781 gctgtggaat cccgcgattc atatagcggc caatgtgtgg gcggttatcc cactgttttt
    12841 ctgggccatg ggcaggagtg gacggccctt tcgcgaaacg tttggtatct atcccgttcg
    12901 ctgggtggtt ctctgggcgg ccacgttcct gggtgccgta accgcgacgc tcactgggca
    12961 cctcggcggc atttttgcgc agggctttcc ttcgtcttct ttcccctttg aggcgtcaga
    13021 ggtgtttcgc gccagtccga acatgagttg tttcgcgctc gtggttgtgg ctccgctcac
    13081 cgaagaactg ctttttcgcg gcgtcatatt gcagggcctg ctgcggcgcc gcagcatcgg
    13141 aaggtcctgt gttgtgagtt ctctgctctt tgcgctgatt catgtcaatc cgtttcagtt
    13201 tgtgcccctg tttctcggag gcttgctgct cgcggccgtg cagctgaaga ccggttccct
    13261 gtggccgagc atatgcatgc acgcgggctt caatattcct ccagcggccg cgatcatggc
    13321 gcaggcgaga ctggctgcaa cgcaggaagc cggcccgccc tctttcccgt ggtttcacgt
    13381 gttcgcaggg gtc
//
LOCUS       DAOPBQ010000027        13343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4635_length_13343_cov_4.287477, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000027 DAOPBQ010000000
VERSION     DAOPBQ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4635_length_13343_cov_4.287477"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKW60_01845"
     CDS             <1..452
                     /locus_tag="PKW60_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPJ98203.1"
                     /translation="VIQVRIPPLRERLDALPQLVGHFVTQMSRTFGKRIQGFDEQAQA
                     YLRNYTYPGNVRELESIIAHAIIMADDDVIRARDLPDQVRAGGSAPFALPHYAADTFL
                     SLKEMEEQLIRQTLHRLDGSQTEAAARLGISRSTLWRKMKEYGISAG"
     gene            complement(510..1559)
                     /gene="mutY"
                     /locus_tag="PKW60_01850"
     CDS             complement(510..1559)
                     /gene="mutY"
                     /locus_tag="PKW60_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017286056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPJ98204.1"
                     /translation="MRRIRRALLAWYTRSARDLPWRRTRHPYPVWVAEIMLQQTRIDQ
                     GTPYFERFMEAFPTVEALASAPEDAVLKLWEGLGYYARARNLHRAARLVAHERGGQFP
                     ETADDWQTLPGIGRYTAGAIASIAFHEAVPVLDGNVKRVLSRLFDIATSIGQAATERR
                     LWALAEALVPARRPGDFNQALMELGAIVCTPKSPSCTICPVRGVCKAFVRGTQAERPV
                     RGKKPAAPHHELVVAAIEKNGRFLLGKRPPSGLLGGLWEFPGGQVEAGETHEQALKRV
                     IRGELGVELRVGGLAAVVNHAYSHFKVTLNVYRCSIASGSPRPVTHTGLKWVPRAWFG
                     RYAFPKANLKFLKAL"
     gene            complement(1619..2581)
                     /locus_tag="PKW60_01855"
     CDS             complement(1619..2581)
                     /locus_tag="PKW60_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98205.1"
                     /translation="MMVNWIAAAAIAMAAAPSGGAATAEAVHWAELKTDALTAVIGDN
                     EPMGDHHRRRYNGVFALATPERAESPFVPMFSGLNLEHYFDAQARTDDDAVFFEPRHV
                     PMELRRIGDNAVELYQATTPFYGVESWTRFTMSAPRYIDMTFRCIPHKNEFIGGFMGV
                     FWASYINAPADKSLYFLQAGSTLDNPQWVQYCTQVHGHASTVVHEADAFRAERGESTG
                     SLFEELSPLRYAEPFYYGLFRDHVLIFIFERGPMVRFSHSPSGAGPTPAGDDTNPAWD
                     FQLIVPGVVAGQEYGLRMRLVYKPWAGRDDVLAEVRNAYATVLK"
     gene            complement(2623..2928)
                     /locus_tag="PKW60_01860"
     CDS             complement(2623..2928)
                     /locus_tag="PKW60_01860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HPJ98206.1"
                     /translation="MLVVLVHVHVKPEYVEAFREATLENARHSVQEPGIARFDVLQQP
                     DRPARFVLIEVYRTEADPARHKETAHYQQWRDTVADMMAEPRQSVRYANCFPGDAGW"
     gene            complement(2951..3694)
                     /gene="queC"
                     /locus_tag="PKW60_01865"
     CDS             complement(2951..3694)
                     /gene="queC"
                     /locus_tag="PKW60_01865"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPJ98207.1"
                     /translation="MRLTLEHRQRELRIPMTALEKPAVVLLSGGMDSSALLHHVVKTL
                     DRRPVHAISCDYGQRHVRELDCAAWQALAAGAEHHRILDVAFLGDLVKGGSSLVHGGA
                     EVPDLASVAAPDREQPSTYVPNRNLILLALAAAYAEARGITDVYYGAQAQDEYGYWDC
                     TAEFVERLNQVLALNRRAPIVVHAPFAAMRKAESLRLGLDLGVDYGHTWSCYRGEGKA
                     CGTCPTCVERLNAFREVGIADPLDYETTR"
     gene            3825..4820
                     /gene="gmd"
                     /locus_tag="PKW60_01870"
     CDS             3825..4820
                     /gene="gmd"
                     /locus_tag="PKW60_01870"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPJ98208.1"
                     /translation="MANSGKPRAVITGITGQDGSYLAEFLLEKGYEVFGVIRRSSTEK
                     FERIKHIQDRITLMHADLTDQGSMIDVITEAQPREVYNLAAQSFVPTSWKQPMLTGQV
                     TALGVTCVLEAIRVVDKSIRFYQASSSEMFGNAQETPQRESTPFYPRSPYGVAKVYGH
                     WITVNYRESYDMFAVSGILFNHESPRRGLEFVTRKISLGVARIKLGLDTEVRLGNLDA
                     KRDWGFAGDYVRAMWLMMQQEIPDDYVVSSGETHSVRDACEAAFSHAGLNWEDHVKVD
                     PKFYRPAEVHLLLGDSTKARNQLQWKPEVSFRDLMGMMVDADIERLQRENRHGTA"
     gene            4817..5761
                     /locus_tag="PKW60_01875"
     CDS             4817..5761
                     /locus_tag="PKW60_01875"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPJ98209.1"
                     /translation="MTMRALVTGADGFVGGVLCRHLAQSGWEVRMGVMRAAQESLAEF
                     QCDITDAERVQALVAWAGPVDVVFHLAAVAFVPDSIQEPQKTLRVNVEGTMNLLQSCR
                     AQLPESRFLFVSTSEVYGPPVELPVRENHPLNPQNPYAISKAAADCYCAYVYESTGYD
                     IVRMRPFNHSGPGQSDQFVLSSFARQLARAEAGVDKPLLRVGNLEARRDFLHVDDVVR
                     AYELAARNGAAGEVYNIASGQAVLIRDALDLLLRMGRVKMTVEPATERMRPVEVREVA
                     GSSERLTRATGWMPQRSLEALLTDVLNFWRAQEGVSAP"
     gene            5784..6743
                     /locus_tag="PKW60_01880"
     CDS             5784..6743
                     /locus_tag="PKW60_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98210.1"
                     /translation="MGFFDTFSNPAVVHALAVHAPIALAFFGVPLVILAAVLVERAAG
                     IRWLAVAAYLGLAAAAFLTVHTGEGARSALPNAAGVIDEPVWNLVRNHQSMAGKVWLF
                     ALVTALLTACTAAQSPRMRLTSGTLAVVSACATAVWLGLTGHYGGTLVYVHGLGTPAL
                     HYKQQLLTQGESPSAAGSSTPAASGFTETAPASAPPESAGPAAAILPPAVVETESAIP
                     APVVRDVSYAQEVAPILREHCYSCHGKTSGLSLETIDAMLVGGRKAGPAVRAGQPEKS
                     PLILYVTGELQPRMPEGGEPLSENDITILRNWIARGMKDDSPG"
     gene            complement(6754..8097)
                     /locus_tag="PKW60_01885"
     CDS             complement(6754..8097)
                     /locus_tag="PKW60_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPJ98211.1"
                     /translation="MKSSLNAILIVVDTLRADHLGCYGYHRNTSPNLDALAGQSVLCE
                     QHITPAIPTHPAFATLGSGQYSITHGIVAHGGRIPLKNTVPWLPALLQKAGYTTCAID
                     NLAPWHHGFGRGYEYYINPASRRALGINCDSRDINSRAIPWLEQNREEPFFLFIHYWD
                     PHTPYMPPRAYRRLFYDAEKDPCDPGNTSLQKLERHPLGKIWRENWLNTLVRGKTVTD
                     ADYVEALYDAEIRYCDEAIGQIIETLDRLGLSERSFLAVTSDHGEMMYRHGIFFDHHG
                     LYEGNLHVPLLLRHPALAPRRVRQLTAHVDLAPTILDFCGVQAPGEMEGFSLAPLMAG
                     ESDAPLRDFVVCQECTWQMKWGLRTLNHKFILARQPDFYGTPLRELYDLREDPREFHN
                     LAGQEPERARQCEAILEEWVAEKMRARGLTRDPLLTHGLTLGREWQRRRPRKHPS"
     gene            8096..9574
                     /locus_tag="PKW60_01890"
     CDS             8096..9574
                     /locus_tag="PKW60_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98212.1"
                     /translation="MHCLTSGAATPILDHGPNVYHDGGGGRKQKITRRLRTGFCGQSR
                     ARVLPAAVEPWYNFLMNEAHPQNPQPRQNRSIIAAVMMLFGGAVLLAVGLGLGFWFAT
                     RYDISISRRGADAPAGYPAPAAQDDSWASTRPGTSGRPAASPRDAATAAPQTATTSDA
                     LWPLSPAEQSQGKADGPFAFKFTRDETLRYRVATEIRGKGVEGLTDSGVLMNFESDMS
                     LHTRDVDPAGNALLRMTFDQTSLRGSFLDEQFSMEVTPNDARIDNRGQTPVDTRRDVG
                     STQGIPQLEFLQEPVDMVVAPNGQVLKLSNTQNMAAMLAAVPRFATLEFPGGTLAPGQ
                     QWESRIALPVPGFGTPAEARLLNTFVGYEHMKGRLCGVIEQQFLSEQTGGAVHAPESA
                     LGEALNLSMPVFQLSGENRVYFDTANGQLVLADMDLGLRMDFSNVLGNLSRALGGLLA
                     NPDALPGASGSDLDELLGLGAGGSSPLLSLDLNLLGSMELTN"
     gene            9783..11126
                     /locus_tag="PKW60_01895"
     CDS             9783..11126
                     /locus_tag="PKW60_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPJ98213.1"
                     /translation="MSVLVNKSVSRTLFSMAFPMLAGTFAMNAYNLTDTWFVSRLGTL
                     SLAAMGFTFPVVMLLTCVARGIGSGVTTLTSHAIGSNDRERAVRLVTHGVMLTFAVTA
                     VMSIGGYLSIGPVFRRLGADSTTLPLIGDYMRTWFTGALFMTLPMAGSGVLISMGDSK
                     AASMLMIAGTLLNTILDPIMIFGFLGCPAMGIRGAALATIISQGVSTIWLLYLLYRRH
                     RLLAWRSWKFADYGASLRQIMQYAIPSVLSMILMPVSASVITRLLSHFGNGAVAAAGA
                     ASRVEMVAFIIPMALGISLTPFVSQNVGAARLDRVRQAKSLSVGFALGYGGFSTAVFF
                     VGAPWFARLFTSDPEVARMFIAYTRIVSFGYGMMEVHRYCGFFLTGLRKPLSATALNA
                     IRVLVLLIPLSWLGSHVWGLEGVFWGRLATDFTVGAIGLLWLSRAFRSVQQDSEA"
     gene            11392..12693
                     /locus_tag="PKW60_01900"
     CDS             11392..12693
                     /locus_tag="PKW60_01900"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPJ98214.1"
                     /translation="MRRNIVHEGAANLSYAIREIVAVAHEIRDLGQPITWENIGDPIE
                     KGERVPEWISEIIKELLDDPKSWGYCDTAGVPQTREFLAGLVNERPGGVQVNPSDIIF
                     FNGLGDAVAKVYGFLRREARILGPSPAYSTHSSAEAAHSGYSHVTYELDPCNGWLPDV
                     DDIRQKVKYNDSIAGILLLSPDNPTGAVYPRDILEEIAEIAREHRLFVIADEIYVHIV
                     YNGNPRMHMSQWIGDVPALAMRGISKEYPWPGSRCGWLEVLNKNRDENFARYVDSLVA
                     AKRLEVCSTTLPQMSIPRIMRDPRYAPHLSQRAAMFDRRADEVVNAFQGCDSVIVTKP
                     GGAFYITVMFKPGVLNDHQTLRIANEPVRRRIEEMVKGVPPDRRFVYYLMGATGIVVV
                     PLSGFHSRHDGFRVTILETDDTRRAWIFQTIRAAIDEYVGS"
     gene            complement(12738..>13343)
                     /locus_tag="PKW60_01905"
     CDS             complement(12738..>13343)
                     /locus_tag="PKW60_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HPJ98215.1"
                     /translation="RIERLFIQRAGLVARDPVRVAWPGEPPYPAWNWWKPEPDHLVVQ
                     WRTRPNVLDYDGDGRQDLIAIDHEGYLALYRNTGDVLEPGKRVFLDEKGEPLRLSDGE
                     AGKSGRAKFHLVDWDGDGDLDLIRNTKNTGWFENTGENRYAWRGDFPIRELAGHTTAP
                     QAVDWNADGKMDLIVGAEDGHIYCYHRAALDEPGKINAVKK"
BASE COUNT         2385 a         4241 c         4248 g         2469 t
ORIGIN      
        1 atgtcatcca ggtgcgcatc ccgccgctgc gcgaacgcct ggatgccctt ccccagctgg
       61 tcgggcattt tgtgacgcaa atgagccgca cgttcggcaa gcgcatccag ggcttcgacg
      121 agcaggccca ggcctatctg cgcaactaca cgtatcccgg taatgtgcgg gaactggaaa
      181 gcatcatcgc ccacgccatc atcatggccg atgacgacgt gattcgcgcg cgcgacctgc
      241 ccgaccaggt ccgcgcgggc ggcagcgcgc ctttcgccct gccccattac gccgccgaca
      301 cctttctctc gctcaaggag atggaagaac agctcattcg ccagaccctt cacagactcg
      361 acggcagtca gaccgaggcc gctgcccggc tgggcatctc ccgctcgacc ttgtggcgaa
      421 aaatgaagga atacggcatc agcgccggct gaggcggccg gcaggccgtg ggcatggggg
      481 tccgcggcgc ccctcattcg ctttctccgc tacaatgcct tgaggaactt caggttggct
      541 ttgggaaagg catagcgccc gaaccaggcc cgaggcaccc acttcaaccc ggtatgcgtc
      601 accggccggg gcgaaccgga agcaatgctg cagcggtaca cattgagcgt taccttgaag
      661 tgcgaatagg cgtggttgac gaccgcggcc agtccgccca cgcgaagttc aacgccgagt
      721 tcgccgcgga tcacgcgttt aagggcctgt tcgtgcgttt ctccggcctc aacttgacct
      781 ccggggaact cccacaaacc tccgagcaga ccggacggcg gccgcttccc cagcaggaag
      841 cgcccgttct tttcaatggc cgctacgaca agttcatgat gcggcgccgc aggttttttc
      901 cctctgacag gacgctccgc ctgggtgccg cggacaaacg ccttacaaac cccccgcacc
      961 gggcagattg tgcaggaagg cgattttggc gtgcagacga tggcgccgag ctccatcagg
     1021 gcctgattga aatcacccgg acggcgcgcc ggcacgagcg cttcggccag tgcccagagc
     1081 cggcgctcgg ttgcggcctg gccgatggat gtggcgatgt cgaagaggcg cgagaggacg
     1141 cgtttcacat ttccgtcaag caccggcacg gcctcgtgga acgcgatgct ggcaatggcc
     1201 ccggcggtat accggccgat gcccggaagg gtttgccagt catccgccgt ctcgggaaac
     1261 tggccgccgc gttcgtgcgc gacgagccgc gcagcccggt gcaggttgcg cgcccgcgcg
     1321 tagtagccca gcccttccca gagcttcagg acggcgtcct cgggcgcgct ggccagggcc
     1381 tcgacagtcg gaaacgcctc catgaaccgt tcaaagtagg gcgtgccctg gtcaatgcgt
     1441 gtctgctgca gcatgatctc cgcgacccac acgggatacg gatgtcgggt gcggcgccag
     1501 ggcaggtcgc gggcgctgcg tgtgtaccat gccaggagcg ctcgacgaat ccggcgcacg
     1561 gcaggagtat caattggcgc ctgactgggt tgggcggctt tgtgcaaggc ccgccccgct
     1621 atttcaatac ggtcgcgtag gcgttccgca cctcggcgag cacgtcgtct cgcccggccc
     1681 agggcttgta gacgaggcgc atgcgcagcc cgtattcctg gccggccaca acgccgggga
     1741 caatgagctg gaagtcccag gccgggttgg tgtcatcgcc ggccggcgtg gggccggcgc
     1801 ccgacggaga atgcgagaaa cgaaccatcg gcccgcgctc aaagatgaat atcaggacat
     1861 ggtcgcggaa aaggccgtag tagaagggtt ccgcgtagcg caggggcgac aactcttcaa
     1921 agagtgagcc cgtgctctca ccgcgctctg ctcgaaacgc atccgcctcg tgcacgaccg
     1981 tgctggcgtg accatgcacc tgcgtacaat attgcaccca ctgcggatta tcgagcgtag
     2041 acccggcttg aaggaaatag aggctcttgt cggcgggggc gttgatgtac gacgcccaga
     2101 acacgcccat gaacccgccg ataaactcgt ttttgtgggg gatgcagcgg aaggtcatgt
     2161 cgatatatcg cggcgcggac atcgtgaacc gggtccagct ttcgacgccg tagaaaggcg
     2221 tggttgcctg gtacagctca acggcgttgt cgccgatgcg gcggagttcc atggggacgt
     2281 ggcggggttc gaaaaacacc gcgtcatcgt cggtacgggc ttgagcgtcg aaataatgtt
     2341 cgaggttgag gcccgaaaac ataggcacaa acggggattc ggcgcgctcc ggcgtggcga
     2401 gcgcgaagac cccgttgtac cgcctccgat gatgatcgcc catgggctcg ttatcgccaa
     2461 tcactgccgt gagggcgtct gtctttaatt cagcccaatg cacggcctct gccgtggctg
     2521 cgccgcccga cggggcggca gccatcgcga tcgccgcggc ggcgatccaa ttcaccatca
     2581 tgcttgttcc tccaggtttc gtaagcgcaa aggccggcgc gcctaccagc cggcgtcgcc
     2641 ggggaagcaa ttagcgtatc gcacgctctg gcgcggctcg gccatcatgt cggccactgt
     2701 atcgcgccat tgctgatagt gcgccgtctc cttgtgccgc gcggggtcgg cttccgtgcg
     2761 gtacacttca atcagcacga agcgggcggg gcgatccggc tgctggagca cgtcgaagcg
     2821 cgcgataccc ggttcctgca cgctgtggcg cgcgttttcg agcgtggcct cccgaaaggc
     2881 ctccacatac tcgggtttta catgcacatg aacaagaacg acgagcatgg ctttgttctc
     2941 ctgcgttcgg tcagcgggtt gtctcgtaat cgaggggatc ggcgatgccc acttcacgaa
     3001 acgcgttcaa tcgttcgacg caggtcggac aggtgccgca ggcctttcct tcgccccggt
     3061 agcagctcca cgtatgcccg taatcgacgc cgagatcgag acccagccgg agcgattccg
     3121 ccttccgcat ggccgcaaag ggcgcatgga ccacgatagg cgcacgccgg ttcagcgcga
     3181 gcacctggtt cagccgttcg acaaactccg ccgtacagtc ccagtagcca tattcatcct
     3241 gcgcctgggc accataatac acatccgtga tgcctcgggc ttccgcatac gccgccgcca
     3301 gcgccagcag tatcaggttg cggttgggca catacgttga cggctgttcc cggtcaggcg
     3361 cagctacact ggccagatcg ggcacctcgg cgccgccgtg aaccagcgaa ctccctccct
     3421 tcaccaaatc gcccagaaac gccacatcga gtatccggtg gtgctcggcg ccggccgcca
     3481 gggcctgcca cgccgcgcag tcgagctccc gcacatgccg ctggccgtaa tcgcaggaaa
     3541 tcgcatggac cggccggcgg tccagggttt tcacgacgtg gtgcagcagc gccgacgaat
     3601 ccatgcctcc gctgagcagg acgacggcgg gtttttccag ggcggtcatg ggaatacgaa
     3661 gctctctttg cctgtgttcc agcgtcagac gcatgatacg gaaaccccgc gggaacgttc
     3721 aatgatcggc tgcaggtctc atgggcgtgc ccgcggttgc ctgacatgcg caggcgtccc
     3781 tataatcagg cttgctgcgg aacagaggga aaggatccaa cattatggcg aattctggaa
     3841 aaccccgggc tgttatcacc ggcataaccg gccaggacgg ctcgtatctg gcggagtttc
     3901 tgctcgaaaa ggggtacgaa gtctttggcg tcattcggcg atccagcacc gagaaattcg
     3961 agcgcataaa acatatccag gatcgcatta cgctgatgca cgccgatctc accgaccagg
     4021 ggtcgatgat cgacgtcatc accgaggcgc aaccccgcga agtctacaat ctggccgcac
     4081 agtcgtttgt gccgacctcg tggaagcaac ccatgctgac gggacaggtg accgccctgg
     4141 gcgtgacctg cgtgctcgag gccattcggg tcgtcgacaa atcgatccgt ttttaccagg
     4201 cgtcatcgag cgagatgttt ggcaatgccc aggaaacgcc ccagcgtgaa tcgacgccct
     4261 tctacccgcg cagcccctac ggcgttgcga aggtctacgg ccactggatc accgtcaatt
     4321 accgtgagag ttacgacatg ttcgccgtgt ccggcatcct gttcaaccac gaatcgccgc
     4381 ggcggggcct ggaatttgtc acgcggaaga tctcgctcgg cgtggcccgc atcaaactgg
     4441 gcctggatac cgaggtgcgg ctcggcaacc tggacgccaa gcgcgactgg ggattcgcgg
     4501 gcgattacgt ccgggccatg tggctgatga tgcagcagga gatccccgac gactacgtgg
     4561 tatcaagcgg cgagacgcat tcagtgcgcg acgcctgcga agccgcgttt tcacatgcgg
     4621 gcctcaactg ggaagaccac gtgaaggtcg atccgaagtt ttaccggccc gcggaagtcc
     4681 atctgcttct tggcgacagt acgaaggccc ggaatcagct ccagtggaaa cccgaggtct
     4741 cgttccggga tctgatgggt atgatggtcg acgccgacat cgagcggctt cagcgcgaaa
     4801 accggcacgg cacggcatga cgatgcgcgc gctagtcacg ggagcagacg ggttcgtggg
     4861 cggggtgctg tgccgccatc tcgctcaatc cgggtgggaa gtgcgcatgg gcgtgatgcg
     4921 cgccgcacag gaatccctgg cagagttcca atgcgatatt accgacgcgg agcgggtgca
     4981 ggcgctggtg gcgtgggccg gaccggtcga tgttgtgttt cacctggcgg cagtggcgtt
     5041 cgtgccggac tccatccagg aaccgcaaaa gaccctgcgc gtaaacgtcg aaggcaccat
     5101 gaatctcctc caatcctgcc gcgcacagct gcctgaaagc cggtttctat ttgtcagcac
     5161 gtcggaagtg tatgggccgc ccgtggagct gcccgtccgc gaaaatcatc cgctgaatcc
     5221 ccaaaacccg tacgccatct cgaaagccgc cgcggactgt tattgcgcct acgtgtacga
     5281 gagcacgggt tacgacatcg tgcggatgcg gccgttcaac cattcggggc ccggccagtc
     5341 cgaccagttt gtgctgtcga gttttgcccg gcagcttgcg cgcgctgaag cgggtgtgga
     5401 taaaccgctg ctgcgggtgg gcaacctgga ggcgcgccgc gattttctgc acgtggacga
     5461 cgtggtgcgc gcgtatgagc tcgccgctcg aaacggcgct gccggggagg tgtacaacat
     5521 tgcttccggg caggcggttt taattcgcga cgcactggac ctgctgctgc gcatggggcg
     5581 ggtgaagatg acggtcgagc ccgcgaccga gcgcatgcgc ccggtggaag tgcgcgaagt
     5641 ggctggttcc agtgaacggc tgacccgcgc gaccggatgg atgccgcaac ggtcgctgga
     5701 agccctgctc acggacgtcc tgaacttctg gcgggctcag gaaggcgttt ccgcgccatg
     5761 atgcgggaag ggagaaccgt ttgatgggat tcttcgatac gttcagcaac ccggccgtgg
     5821 tgcacgcgct ggccgtgcat gcgcccattg ctctggcatt ttttggcgtg ccgctcgtca
     5881 ttctggccgc ggtgctggta gaacgggccg cagggatacg gtggctggcg gtcgcggcct
     5941 atctcgggct cgccgccgct gcctttctca cggtgcacac gggtgaagga gcgcggagcg
     6001 ctctcccaaa cgcagcgggc gtgatcgacg agcccgtctg gaacctggtg cgcaatcacc
     6061 agagcatggc aggcaaagtc tggctcttcg cgctggttac cgccctgttg acggcatgca
     6121 ccgccgcgca aagcccgcgc atgcgcctca cctcgggcac cctggccgtg gtgtcggcgt
     6181 gcgcgaccgc ggtgtggctg ggcctgaccg gccactatgg cggcacgctg gtctatgtgc
     6241 acggtctcgg cacgcccgcc ctgcactaca agcagcagct cctgacccag ggcgaatccc
     6301 cctccgctgc agggtcatca acacccgcgg cgtccggctt caccgaaaca gcacctgcat
     6361 ccgcgccgcc tgaatccgcc gggccggccg ccgccattct cccgccggcg gtggtggaga
     6421 ccgaatccgc gattccagcc cccgtcgtgc gcgacgtgag ctacgcgcag gaggtggcgc
     6481 cgattctccg ggagcattgc tatagctgcc acggcaaaac gagcggcctg agcctcgaga
     6541 ccatcgacgc gatgcttgtg ggcgggagaa aagcagggcc ggccgtccgt gcgggccagc
     6601 cggagaagag tccgctgatc ctgtacgtga ccggagagct gcaaccccgt atgcctgagg
     6661 gcggcgaacc gctgtccgag aacgatatca ccatcctgcg caactggatc gcccggggca
     6721 tgaaagacga ttcgccgggc tgagcggcgc ggcttacgac gggtgctttc gaggccggcg
     6781 gcgctgccat tcgcggccga gggtcagacc gtgcgtgagc agggggtccc gggtgagccc
     6841 ccgggcgcgc atcttttccg ccacccattc ctcgagaatg gcctcgcact ggcgcgcacg
     6901 ttcgggttcc tgcccggcca ggttatgaaa ctcgcgcggg tcttcccgga gatcgtagag
     6961 ctcgcgcaag ggtgtgccgt agaaatcggg ctgccgggcc aggatgaatt tgtggttcag
     7021 cgtgcgcagc ccccatttca tctgccaggt gcattcctga cacaccacaa aatcgcgcag
     7081 cggcgcgtcg ctttcgccgg ccatcagcgg cgccagggag aacccttcca tctcgccggg
     7141 cgcctggaca ccgcagaaat cgagaatggt gggcgcaaga tccacgtggg cggtcagctg
     7201 acgcacgcgc cggggcgcaa gggccggatg gcgcaggagc agcgggacgt gcaggtttcc
     7261 ctcgtacagg ccgtgatgat cgaagaaaat tccgtgacgg tacatcatct cgccgtggtc
     7321 gcttgtgacc gccaggaagc tgcgctcgga caggccaagc cggtcgagcg tctcgatgat
     7381 ctggccgatg gcctcgtcgc agtagcggat ctcagcgtcg tacagggcct ccacgtagtc
     7441 ggcgtcggtc acggtttttc cacggacgag cgtgttgagc cagttctcac gccagatttt
     7501 tcccagcgga tggcgctcga gcttctggag ggaggtgttt ccggggtcac agggatcttt
     7561 ttcagcgtca taaaacagcc gccggtacgc gcggggcggc atgtagggcg tgtgcggatc
     7621 ccagtagtga atgaacagga aaaacggctc ttcgcggttc tgttcaagcc agggaatggc
     7681 ccggctgttg atgtcgcggc tgtcgcaatt gatgccgagc gcgcggcgcg acgccgggtt
     7741 gatatagtac tcatagcccc gcccgaaccc gtggtgccac ggcgcaagat tgtcgatcgc
     7801 gcaggtcgta taccccgctt tctggagcag ggccgggagc cacggcaccg tgtttttcag
     7861 agggattcgg ccgccgtggg ccacgatccc gtgcgtgatc gaatactggc cgctgcccag
     7921 cgtagcgaac gcggggtgcg tgggaatggc cggcgtaatg tgctgctcgc acaagaccga
     7981 ttgcccggca agcgcgtcaa gattaggcga cgtgttgcgg tgatagccgt aacagccgag
     8041 gtggtccgca cgcaaggtgt ccacgacaat tagaatcgcg ttcaaagagg atttcatgca
     8101 ctgtctcact tccggtgccg cgacacccat tttggaccat ggccccaacg tgtatcacga
     8161 tggcggaggc gggcgcaagc agaaaattac gcgccggctc cgcacgggct tctgcggaca
     8221 gtcccgggcg cgtgttctcc cggcggctgt ggagccgtgg tataatttcc tcatgaacga
     8281 ggcacatccc caaaaccctc agcccagaca aaaccgcagc atcattgcgg ccgttatgat
     8341 gctgttcggt ggcgccgtcc tgctcgcggt ggggcttggc ctgggattct ggtttgccac
     8401 ccgctacgac atctcgatct cacggcgcgg cgccgatgca cccgcaggat atccggctcc
     8461 ggcggctcag gatgattcgt gggcgtccac ccgcccgggc acatccgggc gcccggcagc
     8521 ttcgccacgt gacgctgcta cagcagcgcc gcagaccgcc accacctccg acgccctgtg
     8581 gccgctgagc ccggcggagc agagccaggg caaggccgac ggcccgtttg ccttcaagtt
     8641 cacccgggat gaaaccctcc gctaccgcgt tgccacggaa attcgcggca aaggcgtcga
     8701 aggactgacc gattcgggcg tgttgatgaa cttcgagagc gatatgtcgc ttcatacccg
     8761 cgatgtggac ccggcaggca acgcgctcct gcgcatgacc ttcgaccaga cctcgctccg
     8821 gggcagcttt ctggacgagc agttctcgat ggaggtgacg ccgaacgacg cgcgcattga
     8881 caaccgcggt cagacccccg tggatacccg gcgcgatgtc ggctcaacgc agggcatccc
     8941 gcagctcgaa tttctgcagg aaccggtcga catggtggtc gcgccgaacg gccaggtgct
     9001 caaactctcc aatacacaga atatggccgc aatgctcgcc gccgtacccc gtttcgccac
     9061 gctcgaattt ccgggaggaa cgctggcgcc gggacagcaa tgggaaagcc gcatcgcgct
     9121 gcccgtgccg ggtttcggca cacccgcgga agcgcggctg ctcaacacct tcgtgggcta
     9181 tgagcacatg aaagggcggc tctgcggcgt gatcgagcaa cagtttctgt ccgaacagac
     9241 cggcggcgcc gtccacgctc cggaaagcgc actgggagag gccctcaatc tctcaatgcc
     9301 cgtctttcag ctgtccgggg aaaaccgcgt ctatttcgac accgccaacg gccagctcgt
     9361 gctggccgat atggatcttg gcctgcgcat ggatttcagc aacgtgctcg gcaatctgtc
     9421 ccgggcactg ggcggattgc tcgctaatcc ggacgcgctg cccggggctt ccgggtctga
     9481 tcttgacgag ctgctcggcc tgggcgccgg aggctcgtcg cccttgctca gcctggacct
     9541 gaacctcctg ggttcaatgg aactgaccaa ctagcacgag cggaaagcag ccaatcccgg
     9601 caaaagtgcg ctggcgaact cacagcctcc agagactggc cctgcgagtt ttgcgcgctg
     9661 caaaccgccg tggccttgct ttgcatggag tccgcataga tactattcag acctgcgcgc
     9721 gccatgtcct gcatccgctg gcgggagaac accgaaattt cacgggaatg caatgaggaa
     9781 aaatgtctgt attggtcaat aaaagcgtga gccgcacgct gttttcgatg gcatttccga
     9841 tgctggccgg cacctttgcc atgaacgcct acaacctcac cgacacctgg ttcgtgtcgc
     9901 gcctgggcac gctgtccctg gcggcaatgg ggttcacgtt ccccgtggtg atgctgctga
     9961 cctgcgttgc gcgcggtatc ggctcgggcg tgaccacgct gacctcccat gcgatcggca
    10021 gcaacgaccg cgaacgcgcg gtccggctgg tcacgcacgg ggtgatgctc accttcgccg
    10081 tcacggcggt gatgtccatc ggcggctacc tcagcattgg acccgtgttt cgacgcctgg
    10141 gcgcagacag caccaccctg ccgctgatcg gcgactacat gcggacctgg ttcacgggcg
    10201 ccctgttcat gacgctgccg atggcgggca gcggcgtttt gatctccatg ggcgattcga
    10261 aggcggccag catgctcatg atcgcgggca cgctcctgaa cacgattttg gaccccatca
    10321 tgatcttcgg gttcctcggc tgcccggcca tgggcatccg gggcgccgcc ctggcgacga
    10381 tcatctcgca gggggtgtcg acgatctggc tgctctacct gttgtaccgc aggcaccgtc
    10441 tgctggcctg gcgctcctgg aaattcgccg attatggggc ctcgctgcgg cagattatgc
    10501 aatatgcgat acccagcgtg ctcagcatga tcctgatgcc ggtctccgcc agcgtgatca
    10561 cgcgcctcct gagccacttt ggaaacggag cggtcgccgc ggccggagcg gccagccgcg
    10621 tcgagatggt cgcgttcatc atcccgatgg ccctcggcat ttcgcttacc ccgtttgtga
    10681 gccagaatgt cggcgcggcc cgcctggacc gggtcaggca ggccaaaagc ctgtccgtcg
    10741 gcttcgctct gggctacggg gggttctcca cggccgtatt cttcgtcggc gcgccgtggt
    10801 tcgcgcgcct cttcaccagc gaccccgagg tcgcccgcat gtttatcgcc tacacgcgca
    10861 tcgtctcttt tggctacggg atgatggagg tccaccgcta ttgcggattc ttcctgaccg
    10921 gcctgcgcaa accgctctcc gcaaccgccc tcaatgcgat ccgggtcctt gtcctgctga
    10981 tcccgctttc ctggctcggt tcccatgtct gggggcttga aggcgtgttc tggggccggc
    11041 tggccacgga tttcacggtg ggcgccatcg gccttctctg gctgtcccgc gcatttcgct
    11101 ccgtccagca agacagcgag gcataagcca ttttccacgt ttgggggagc cgtttccgcg
    11161 aacccttcac gagacaccgg cgccaccggg catgccgtcc cgcgtcggcg tgttgcatca
    11221 ttcgcgctgg agccgtatga tctgctctta tgttttcctg ccccccgatg gtcgcgtgag
    11281 acaatacgca taccctgggg ttcatctata cggacatcct gagcattcgg cccgtgtgca
    11341 gggcgcgggc aggagcgtgc caaccaatgg attgtcggga gttgccaatt cgtgcgacgt
    11401 aacattgtac atgaaggtgc ggccaatctg agctatgcca tccgcgagat cgtggcggtg
    11461 gcgcatgaga tccgcgatct gggacagccc atcacctggg aaaacatcgg tgacccgatc
    11521 gaaaaagggg agcgggtgcc cgaatggatc agcgagatca tcaaagaact gctcgacgac
    11581 cccaaatcgt ggggctattg cgatacggcg ggtgtgccgc aaacccgcga gtttctcgcc
    11641 ggcctcgtga acgaacgtcc gggcggggtg caggtaaacc cgagcgatat catctttttc
    11701 aacggtctgg gcgacgccgt ggcgaaggtc tacgggtttc tgcggcgcga ggcgcggatc
    11761 ctgggcccat cgcccgcata cagcacccat tcgtcggcgg aagccgcgca ttcgggatac
    11821 agtcacgtca cctacgaact tgatccctgc aacggctggc tgcccgatgt ggatgacatc
    11881 cgccagaagg tgaaatacaa cgactccatc gcggggatcc tgttgttgtc accggacaac
    11941 cccaccggcg cagtgtatcc gcgcgacatc ctcgaggaga tcgctgagat cgcccgcgaa
    12001 caccggctct tcgtgatcgc cgacgagatc tacgtgcaca tcgtctacaa cggcaatcca
    12061 cgcatgcaca tgagccaatg gatcggcgac gtgcccgccc tggccatgcg cggcatcagc
    12121 aaggaatatc cctggccggg ttcacgctgc ggctggcttg aagtgctgaa caaaaaccgc
    12181 gacgagaact tcgcgcgtta cgtggacagc ctggtcgccg cgaaacggct cgaggtctgc
    12241 tcgacgacgc tgccgcagat gtcgattcca cgcatcatgc gcgacccgcg ctatgccccg
    12301 cacctcagcc agcgggcggc catgtttgac cggcgcgccg atgaggtcgt gaatgcgttt
    12361 cagggatgcg attcagtcat cgtcaccaag cccggcggtg cgttttatat caccgtcatg
    12421 ttcaaaccgg gcgtgctcaa cgatcaccag acgctgcgga tcgccaacga accggtgcgc
    12481 cgccgtatcg aggaaatggt gaagggcgtt cccccggacc gccggtttgt gtattacctc
    12541 atgggcgcca cggggatcgt cgtggtgccg ctgtcggggt tccacagccg ccacgacggc
    12601 ttccgcgtga ccatcctcga aaccgacgac acgcgccgcg cgtggatttt ccagaccatt
    12661 cgcgccgcca tcgacgagta cgtggggagc tgacgccgcc ggacctccgt ctgtgcgctg
    12721 atccgcaagc cctgcagcta ttttttcacg gcgttgatct tgccgggttc atccagagcg
    12781 gcccggtggt agcagtagat gtgcccgtct tccgcgccga caatcaggtc catcttgccg
    12841 tccgcgttcc agtcgacggc ctgaggcgcg gttgtgtgtc cggccagttc ccgaatggga
    12901 aaatcgcccc gccaggcgta gcgattctcg ccggtgttct caaaccagcc cgtgtttttc
    12961 gtgttgcgaa tgagatccag gtcgccgtcg ccgtcccagt cgacgagatg aaacttggcg
    13021 cgcccgcttt tgccggcttc gccgtcgctc aggcggagcg gttcgccctt ttcatcgaga
    13081 aagacgcgtt ttcccggctc gaggacgtcc ccggtgttgc ggtaaagcgc aagataaccc
    13141 tcgtgatcga tggcgatgag gtcctggcgg ccgtccccgt cgtaatcgag cacattgggc
    13201 cgggtgcgcc attggacgac caggtggtcc ggttcgggct tccaccagtt ccaggcggga
    13261 tacgggggct caccgggcca cgcgacgcga acggggtcgc gcgccaccag cccggcccgc
    13321 tggataaaga gccgttcgat gcg
//
LOCUS       DAOPBQ010000028        13228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4676_length_13228_cov_4.270477, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000028 DAOPBQ010000000
VERSION     DAOPBQ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 13228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13228
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4676_length_13228_cov_4.270477"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PKW60_01910"
     CDS             <1..504
                     /locus_tag="PKW60_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LmbE family protein"
                     /protein_id="HPJ98216.1"
                     /translation="RPFPVVIVDIRAYHAREDLAANNSVLLDYVRQGGTALVMYQKTF
                     EWKPEYAPYPIQVSTNRVGAENAPVTLLVPDHPLFTTPNAIAPEDWLGWVQERGLYIP
                     DAWDEHYTPLVRTNDPGEDIPPGSCLICDYGEGIYLYTSLVWYRQLREAHPGALRVFA
                     NMLALGK"
     gene            571..1377
                     /locus_tag="PKW60_01915"
     CDS             571..1377
                     /locus_tag="PKW60_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPJ98217.1"
                     /translation="MSKPVAFAVAAHPDDIEFMMGGTLLLLGKAGYDLHYMNIANGSM
                     GTAIYSREEIIAMRTGEARRAAKAMGATFHEPLVDDLDIYYDRELPGRLCAVIREIKP
                     RIMLLQPPTDYMEDHTNACRLAVSAAFFRGMRNLITDPPRDPIADPVTLYHALPYGLH
                     DPLRRRVWPGLYVDITTVIDKKREALACHTSQKEWLDVSQGMDSYLITMEEMSREVGK
                     MSGIFEYAEGWRRHLHLGYCAPEDDPLRDALGHKALVSRTYEEALEKEPQ"
     gene            1530..2774
                     /locus_tag="PKW60_01920"
     CDS             1530..2774
                     /locus_tag="PKW60_01920"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPJ98218.1"
                     /translation="MPRTLYFKLSAMMFLQYAIPACINPIMSLYLMNNLGFPPGQTGQ
                     ILAVVAIGAIVAPFLATYVVDRFISARILLAACHGLAGAVIGVLSASRAFWPVYVLYF
                     VYGLLFLPTHGLSNTVVLHRIRDARRDFGSIRMWGTAGWVFIAWTFGLFWLKGAPPGE
                     ADPRLVDALRLCALLHLALAVYSLFLPREERAQDAPISLMPWEAFRVFARPSVMVLCI
                     ATLCTSAIHQHYYFGMAPFLKSLGAAENHIMPLMSIGQVAEVLMLGLLGRALVRLGTK
                     RVLIIALAAQAARFTVFSIGSPFPLVVAVITAHGFCYAFFFTASYIYIDEHSSREARA
                     GAQQLFTILIMGVGYFLGHVTAGRLGEWSLDPVTEAIRFRQYWAVPAVSAVVIALFLL
                     VAFRAETPELNEVPDSAAALES"
     gene            complement(2808..5954)
                     /locus_tag="PKW60_01925"
     CDS             complement(2808..5954)
                     /locus_tag="PKW60_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPJ98219.1"
                     /translation="MIPPKALEKLKQRIARLGEWRYATAAEVPLEGLETLDHLRVPPG
                     LKSDATSRFKPAPVGSRWGQEWGSVWFRGEARIPSEYAGRRVYYRMDSFADKLLFVNG
                     KPFAGMNIWHREVLLTAQATGRERFSLHIEQYSGHRMPNLRPLDPFEYFHQFTGADPG
                     QPPPLELKASELVVEREAVSSLYYGADVLFRTALILDEHSLRRARILDDLNRALDLIP
                     FHWESETELEDAAKAAARLIAPLLALQNGPTTPLVGLAGHTHIDVAWLWPIAESIRKT
                     ARSFTSMLHLMEHYPEMRFLQSQPALLDLIQNEYPDVIKRIQAEVKTGRWEPNGGMWV
                     EADCNLTGGESLVRQFLEGRKKTRELFNYTSDTLWLPDVFGYSAALPQILRKCGILNF
                     VTSKINWNDTTRFPYDTFWWQGIDGSEVFTHYITCRNGGYNAEVGPEISKDCWDHVQQ
                     KELQDATLASVGYGDGGGGVTREMCERARRMGDLEGCIKTAFVNVSEFLARLREQKTK
                     RPRWVGELYLEIHRGTYTAQARTKQYNRRLELLLREVELLSVQAMPFGLKYPAKKLER
                     LWRTLLTHQFHDILPGSSIRRVYEDAEAAYASMEKDLSQLKRKAFKSLAEHIDTTGTG
                     TPYLISNALSWPREGIVFIKCDKGLVVDDAEGQPVPCQWVCDGKTRGLAIAVTIDALS
                     TMPIYLRQGTDAPMSPFACSDRTLETPYYQVKFDTAGKITSLIDRSVDRELVQAGRRL
                     NDLYTAEDLPLNWDAWDIDLHYRDTLVPQDRMISREVVTEGPLCITIRSKYELGRRST
                     LVQDMTFYAASPRIDFKTWVDWQETHTLLKAGFPLDLNAPQFRCEIQYGHVTRNTHSN
                     TPFDQAQFEVCAHKWVDLSEGDYGVALLNDSKYGHDTLDGMLSITLLKSPVAPDPLAD
                     RGEHTFTYALLPHLGSFTAEEVVREAYNLNVPLALMQAGHRTAEAQALRFCSVSNPNV
                     VIEAVKKAEESNAVIVRLYEAGNTRGQTTLTFDRRIKQASECSLLEDGDTPVKARRRS
                     ITLPIAPFEIKTLKIVFE"
     gene            complement(6000..6650)
                     /locus_tag="PKW60_01930"
     CDS             complement(6000..6650)
                     /locus_tag="PKW60_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98220.1"
                     /translation="MKSRNVGSVLILALAAAWCTGGAWAAGSGPEVTVSISGSLNDML
                     PVLQLLRNMGFGFESPDDAEKPLRLEVHSVVTDGDLIEGAAAGDIPGMGERPDQAALP
                     DTPGLYHPAADPASAKPGDHITVSAMLVDPERRVDTMSARIVGAEGVLADLYNDGTHG
                     DATPNDATWTGTLILPSTLLTGSHLVELAAYDNYGDPVAGENGEPMRLRIPIEITK"
     gene            complement(6727..11412)
                     /locus_tag="PKW60_01935"
     CDS             complement(6727..11412)
                     /locus_tag="PKW60_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPJ98221.1"
                     /translation="MNIHPLAELAAGHPVTRWLVLGPFVVQTSPQFEREYLYERERIL
                     DIDYLADDGGETAVRPEPGRVHRNPGLGPAQLRWSACEGAELDGERIAGEAIYETVQR
                     NCVIYAAAFIEAATDTSALLDAQHSGMKAWLNGQLVCNEPYGFPKGVRLTRPGTRVSL
                     KAGLNLLLIKFRPGYIADGIDFRVRDVRLSPLVSERGLPIALGRVRALPRFTGTPEQP
                     RQLIEVAAINTSRVDVRATFTCVSSAPTAEDKINAVLPPGRITTLRLSLPAVPEGTTV
                     DATVTARLYGRELAAPFQYEAAAPPEGAGVEMVLTSFHFDTTYHEEQRVYAMGAFDIV
                     RQYCRLHRTDPQFRSTLSEVDYLKPYFDVFPDDRETLIRAFRENRSNSDAMYNQPHAM
                     NCGGEALVRNFLYGQLFHGRVLGRICHVYAPGDVFGHPNQLSQIARKAGCLGVSWDKY
                     IYNFPPLFQHLALDGSSLPHKRGNWGWSEAADMGLSVIVGDIDQTPPTDWHETLLPKV
                     QQATYRDLFDIVVRQCEESGACLPVTSRDMSLYHAATAVSRVNLKIANRLGENVLLMA
                     ERFATLAGLLGAVYPDKALDKAWRQLLCGHHHDSITGTHNELSYIDLMNSYREALELG
                     SDVLDRSLHYLGKAVAPRGDDQPLVVFNPLAWERTDVVRAQIHVGGLTQFAITDHRGR
                     EVPFELLDAARNRSGGITEARVAFVAKDMPSLGYRRYQVTPSEKEPKERKTTAGRTSI
                     ENKFYRITVDPARGGGLVSIYDKKAKREVLQQGAHVGNELAALQEIADREETQHEFYT
                     TGLKLFSGEIPASVTAIRGPVSETLRVTYRMGELCGVTQDITLTKGVARIDFQTILED
                     YQGEDHLFCVTFPVNLAGATPVFDERFGAVSRGSSRGYLDFRTHQMAMFSGCAVYAAN
                     KWMDYGPSVQLKAGRNAYPVSMVGLITPKTSEITGIAEGLQRALIQRGVTCTPWHDTG
                     GPYWGSYQDHMDDDLLYTRFRISLGVHHNNQYSNHLLNRQGAKTVAEFQRQLNEEGFA
                     YLFTSDSELEDLSWGPMPVLVIEAKTPKDLASACDRLLSDLRDAAVLRIPGKCDATGW
                     KGVVDEYGVALLNEGTYANSIERNGTLCMMLCHTCRWYGSTATFPEGYLVPENKNHVF
                     RYALYPHSGDWRKANTQHAAHEFNFPLLAREVKPAAKAILPPEQAFLTVSPSNVILAA
                     MKPAGNPLPAFQRTVASNPADGITLRLYDTEGVDSTARITFAAGMSDAWMANLLEEES
                     RPLPIRENGLDVPVPPFSIETVGFVPNPLGPKAGRRTLGTQAEPVQPVWVRSWEHDAE
                     SLPMGYGAVTCTLGREVREEDHGRVLRVMVNAVNDYTDAAIAVTANVAVPQGWQASPG
                     ALAFELPALGHGTKEVQITRPEADAPGQVKLRYEFDGQTFQDVLEIGEAFELTMDAAH
                     EGGDIVVRLTNPACEDIDAEVALVAPLEAWPARDAGDYSLVSITPRTQGVTVAPQSTA
                     TLRFQVACPALGESTRLHSYWAVAKLMSNGRITLARCDQRPERRLMYARKWNSMLKEK
                     ARRRRENER"
     gene            complement(11635..12894)
                     /locus_tag="PKW60_01940"
     CDS             complement(11635..12894)
                     /locus_tag="PKW60_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPJ98222.1"
                     /translation="MTGGRMRTAARSAPVALTLLGLLVSGYLFGRTLTLLMSQDPDAF
                     DVCSAIFGAGCDKTLLSATSWVLGLPLAGWGIVYYGALLMLFFMARALGDEFAPQSLT
                     AALMMSVAGAMSSLVLATLLLGGWAPFCPLCMVVHVINLLLAAALKIRLGSSWAGVAK
                     ALASALRPLFQKPAGRPPLWRWRATGLFTAALTALALYEGLFILSERWLCESRDVFSP
                     HRLLAAYADAPEIELPVSPANPRRGSEHAPVEMVVFSSFHCPACRQFSREMRVLMDSN
                     PGAISLVFRHFPLDGACNPSAGGEAGESGACETAFAAAAAQRQGKFWEFHDTLFKTHF
                     PPTPEALEAVAAEIGLDLARFNAERASEEVRAAVRQDIELGIQLQVQSIPTIFINRRS
                     LPGIGARSIEIIIKDTLKNGAAPHGAR"
     gene            complement(12991..>13228)
                     /locus_tag="PKW60_01945"
     CDS             complement(12991..>13228)
                     /locus_tag="PKW60_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98223.1"
                     /translation="AYLESRDTGYEAQQARTAGGGIAITPEPVNTYRAEVEAFSRAVL
                     EGTEPPVSAEDGLWSQYVLAACYESARTGAAVSL"
BASE COUNT         2251 a         4155 c         4228 g         2594 t
ORIGIN      
        1 cggccgttcc cggtggtgat tgtcgatatc cgcgcctacc acgcgcgcga ggacctggcc
       61 gcgaacaatt ccgtgttgct ggattacgtg cggcaggggg gcacggcgct ggtcatgtat
      121 cagaagacct tcgagtggaa acccgaatac gcgccgtatc ccatccaggt gtcgaccaac
      181 cgggtgggcg ccgagaacgc cccggtcacc ctgcttgttc ccgatcatcc gctgtttacc
      241 acccccaacg cgatcgcgcc cgaagactgg ctgggctggg ttcaggagcg cgggctgtac
      301 atccccgacg cctgggacga gcactatacg cccctcgtgc gcacgaacga tcccggcgag
      361 gacattcccc ccggaagctg cctgatctgt gactacggcg agggcattta tctgtacacg
      421 tcgctggtat ggtaccgcca gcttcgcgag gcgcatcccg gggcgttgcg cgtgttcgcc
      481 aacatgctgg cgctggggaa gtgattttcg gacgaggggt acggcatact gtggggcctt
      541 cagcgcgcat aacgaagaga ggaattcatc atgtccaaac ctgttgcctt tgccgtggcc
      601 gcacacccgg atgatatcga attcatgatg ggcggcacgc tgctgcttct cgggaaagcc
      661 ggatacgatc tgcattacat gaatatcgcc aacggctcga tgggcacggc gatttattcg
      721 cgcgaggaga tcatcgccat gcgcaccggc gaagcccggc gggctgcaaa ggccatgggc
      781 gcgacgtttc atgagccgct ggtggatgac ctcgacattt actacgaccg ggagctgccg
      841 ggccgcctgt gcgcggtgat tcgggagatc aaaccgcgga tcatgctgtt gcaaccgccg
      901 acggattaca tggaagacca caccaacgca tgccgcctgg ccgtttcggc ggcatttttc
      961 cgcggcatgc gcaatctcat cacggacccg cccagggatc ccatcgcgga cccggtgacc
     1021 ctctaccatg cgctgccgta tggcctgcac gacccgctgc gcaggcgggt gtggcccggg
     1081 ctgtatgtcg acatcacgac ggtcattgac aagaagcgcg aggccctcgc ctgccatacc
     1141 agccagaaag agtggctcga cgtgagccag ggcatggaca gctatctcat cacgatggag
     1201 gagatgtcgc gcgaggtggg caagatgtcg ggcatcttcg aatatgccga aggctggcgc
     1261 cgtcatctgc acctggggta ctgcgctccg gaggatgatc cgctgcgtga tgccctgggc
     1321 cacaaagccc tggtgtccag gacatacgaa gaggccctcg agaaagaacc ccaataaacc
     1381 cgccggcggg gtctcgcgcg cttctcgtgc tatggcacgg gaacgcgcgg tcgcgccgcg
     1441 gatcgcgttt tcagcgatct ctccgttgaa cgctcatgga gaggttccgc cggcggtttc
     1501 gcggcccgcc cggctccggg agagatgcat tgccccgtac gttgtatttc aagctctcgg
     1561 cgatgatgtt cctgcagtac gccatcccgg cgtgcatcaa tcccatcatg agcctgtacc
     1621 tgatgaacaa cctcggtttc ccgcccggcc agaccggcca gatccttgcc gtcgtggcca
     1681 ttggagctat cgtcgcgccg tttctggcca cctacgttgt ggatcggttc atttccgcgc
     1741 ggatcctgct cgccgcatgc cacggcctgg caggcgcggt catcggggtg ctttcggcgt
     1801 cgcgcgcctt ctggccggtc tacgtgctgt atttcgtcta tggtctgctg ttcctgccga
     1861 cccacggtct ctccaatacg gtcgtgctgc atcgaatccg cgacgccagg cgcgattttg
     1921 gcagcatccg catgtggggc acggcgggct gggtgttcat cgcctggacg ttcggcctgt
     1981 tctggctgaa aggggcgcct cccggcgagg ctgacccgcg cctggtggac gcgctgcgac
     2041 tgtgtgcgct cctgcatctg gccctggcgg tttacagcct tttccttccc cgcgaggagc
     2101 gagcccagga cgcgccgatc agcttgatgc cgtgggaggc cttccgggta tttgcccggc
     2161 ccagcgtgat ggtcttgtgc attgcgacgc tgtgcaccag cgccatccat caacactact
     2221 atttcggcat ggcgcccttt ctcaaaagcc tgggcgccgc tgaaaaccac atcatgccgc
     2281 tgatgagtat tgggcaggtt gccgaagtcc tgatgctggg gctgctgggc cgcgccctgg
     2341 ttcgcctcgg cacgaaacgg gtattgatca ttgccctcgc cgcgcaggcc gcgcgattta
     2401 ccgtcttcag catcggctcg ccgtttccgc tcgttgtcgc ggttatcacg gcgcatggtt
     2461 tctgttacgc cttctttttc accgcctcgt atatctatat cgacgaacac agctcccggg
     2521 aggcccgtgc cggcgcgcag cagctgttca ccatcctgat catgggtgtg ggctatttcc
     2581 tgggccatgt caccgcaggc aggctcggcg aatggtctct tgatccggtg acggaggcga
     2641 ttcgtttccg ccagtattgg gcggttccgg cggtgagcgc cgtagtcatc gccctgtttc
     2701 tgctggtggc tttccgtgcc gaaacgcccg agctcaacga ggtgcctgac tccgccgcgg
     2761 cgctggagtc gtgagcgcgc cacgcgggtg cccgcccggg cacaagccta ttcgaacacg
     2821 atcttcaggg tctttatctc gaagggggcg atcggcaggg tgatgctgcg gcggcgcgcc
     2881 ttgacgggcg tgtcgccatc ttcgagcagg ctgcattccg acgcctgctt gatgcgccgg
     2941 tcgaacgtca gggtggtctg gccgcgcgta ttgccggctt cgtacaggcg gacgattact
     3001 gcgttgcttt cctcggcttt tttgacggcc tcgatgacca cgtttggatt ggagaccgag
     3061 cagaaccgca acgcctgcgc ttcggctgtc cggtggcctg cctgcatgag cgcaagcggg
     3121 acattgaggt tataggcttc gcgcaccact tcttcggcgg taaacgagcc caggtgcggc
     3181 agcagcgcgt aggtgaacgt gtgttcgccc cggtcggcca gcggatcggg cgccaccggc
     3241 gatttgagca aggttatcga cagcatgccg tcgagggtgt cgtggccgta tttgctgtcg
     3301 ttcaacaggg caacgccgta gtcgccttcg gacagatcga cccacttgtg cgcgcatacc
     3361 tcgaactgcg cctgatcaaa cggcgtgttg ctgtgtgtat tgcgggtcac atggccgtac
     3421 tggatctcgc agcgaaattg gggtgcgttc aaatccagcg ggaacccggc cttgagcagg
     3481 gtatgcgttt cctgccagtc cacccacgtc ttgaagtcga tccgcgggct cgctgcgtaa
     3541 aaggtcatat cctgcaccag cgtggaccgg cgccccagct cgtattttga gcggatggta
     3601 atgcacaggg ggccttccgt gaccacctcg cgcgaaatca tccggtcctg gggcacgagt
     3661 gtgtcgcggt aatgcaggtc gatgtcccag gcgtcccagt tcagcggcag gtcctcggca
     3721 gtatacaaat cgttcaggcg gcggccggcc tgcaccagtt cgcgatccac ggaacggtct
     3781 atcaggctgg tgatcttgcc cgccgtatcg aatttgacct ggtaatacgg ggtttccagc
     3841 gtccggtcag aacaggcgaa gggcgacata ggcgcgtcgg tgccctggcg caggtagatg
     3901 ggcatggtgc tcagggcatc gatagtcact gcgatggcga ggccgcgcgt cttgccgtcg
     3961 cacacccact ggcacggcac gggctgtcct tcggcgtcgt cgacgacaag gcctttgtcg
     4021 cacttgatga acacgatgcc ttcgcgcggc caggacaggg cattcgagat gaggtacggc
     4081 gtgcccgtgc cggtggtgtc gatatgttcg gccagacttt tgaacgcttt ccgtttgagc
     4141 tggctcaggt ctttttccat cgacgcatag gccgcctcgg catcttcgta gacccggcgg
     4201 atcgaggagc cggggaggat gtcgtggaac tggtgggtca gcagggtgcg ccagaggcgc
     4261 tcgagttttt tggccgggta cttcagcccg aatggcatgg cctggacgga caggagctcg
     4321 acctcgcgca acaggagttc gagccggcgg ttgtattgtt tcgtgcgggc ctgggccgta
     4381 tacgtgccgc ggtggatttc gagatacagt tcaccgaccc agcgcggccg tttggtcttc
     4441 tgctcgcgca gccgcgcgag gaattccgag acgttcacaa acgcggtctt gatgcagcct
     4501 tcgagatcgc ccatgcggcg cgcgcgttcg cacatctccc gggtgacgcc gccgccgccg
     4561 tcgccataac cgaccgacgc cagcgtggca tcctgcagct ccttctgctg cacgtggtcc
     4621 cagcagtctt tggaaatttc ggggccaacc tcggcgttgt agcctccgtt gcggcaggtg
     4681 atgtaatgcg tgaatacttc gctgccgtcg atcccctgcc accagaacgt gtcgtatggg
     4741 aaacgcgtgg tatcgttcca attgattttg ctggtgacaa agttgagtat cccgcatttc
     4801 cgcaggatct gcggcagggc ggccgaatag ccgaagacgt cgggcagcca cagggtgtcg
     4861 ctggtgtaat tgaacagttc gcgcgtcttt ttgcgcccct ccaggaactg gcgcacgagt
     4921 gattcgccgc ccgtgaggtt gcagtcggct tcgacccaca tgccgccatt gggttcccag
     4981 cgcccggtct tcacctccgc ctggatgcgc ttgatgacgt cgggatattc gttctgaatc
     5041 aggtcgagca aggccggttg cgactgtaaa aaccgcattt ccggataatg ttccatgaga
     5101 tggagcatgc tcgtgaacga tcgggcggtc ttgcggatgg attcggcgat gggccacaac
     5161 caagccacgt cgatgtgggt atggcccgcc aggcccacga ggggtgtcgt agggccattc
     5221 tgaagcgcga ggagcggcgc aatcaggcgt gccgccgcct tcgccgcgtc ctccagctcc
     5281 gtttcggatt cccaatggaa cgggatcaga tccagggcgc ggttcaggtc gtcgaggatg
     5341 cgcgcgcggc gcagcgaatg ctcatcgagg atcagcgctg tgcggaacag cacgtctgcg
     5401 ccataataca gcgacgagac agcctcgcgt tcgacgacca gctcgctggc tttgagttcg
     5461 agcgggggcg gctggcccgg atcggccccc gtgaactgat ggaaatactc aaaggggtcc
     5521 aggggccgca ggttgggcat gcggtggccc gagtattgtt cgatgtgcag cgagaaacgc
     5581 tcccggccgg ttgcctgagc cgtcagcagc acttcgcggt gccagatgtt catgcccgcg
     5641 aacggtttgc cgttcacgaa gaggagcttg tccgcgaacg aatccatgcg gtaatagaca
     5701 cggcggccgg cgtattcgga tggaatccgc gcctcgccgc gaaaccacac cgaaccccat
     5761 tcctggcccc agcggctgcc gacgggcgcc ggcttgaacc gggacgtggc atcgcttttc
     5821 agacccggcg gcacgcgcaa gtgatccagc gtctccaggc cttcgagggg cacctcagcg
     5881 gctgtcgcat accgccattc gccgagccgg gcaatacgtt gtttcaattt ctctaatgct
     5941 ttcggcggga tcatggactc tcctcataaa ggcatggggg atgcggcgca agcacacgcc
     6001 tatttggtaa tttcgattgg gattcgcaga cgcatcggtt ctccattttc tccggcgacc
     6061 gggtcgccat aattatcgta ggccgctagc tcgacgagat gcgaacccgt aagaagggtg
     6121 gacggcagga tcagggtgcc ggtccaggtc gcatcattcg gcgtggcgtc tccgtgggtg
     6181 ccgtcgttgt acagatcggc gagcacacct tccgcgccca caatccgggc gctcatagtg
     6241 tcaacgcgcc gttccgggtc caccagcatg gccgacaccg tgatgtggtc gccgggtttt
     6301 gcgcttgcgg ggtcggccgc ggggtgatag agcccgggcg tgtccggcag ggccgcctga
     6361 tcagggcgtt cgcccattcc ggggatatcg ccggccgcgg cgccctcgat aagatcaccg
     6421 tcggttacga cgctgtgcac ttccagccgc aacggcttct ccgcgtcgtc tggcgactca
     6481 aatccgaacc ccatgttgcg aagcaattgc agaaccggga gcatgtcatt caacgagccg
     6541 ctgatggata ccgtgacttc cggacccgaa ccggccgccc aggcgccgcc ggtacaccag
     6601 gccgccgcca acgccaggat cagcacgctg ccaacgtttc tgctcttcat tgtcgttccc
     6661 ctttcgcaat cccacttgac ctcttcagga gcccctgcgg agagacagcg cccgacgagc
     6721 ggcatctcac cgctcatttt cccggcgtct gcgcgccttc tctttcagca tgctattcca
     6781 cttgcgcgcg tacatcaagc gacgctctgg gcgctgatcg caacgcgcca gcgtaatacg
     6841 gccgttggac atcagtttgg ctacggccca atacgaatgc aaccgcgtgc tttcacccag
     6901 ggccgggcag gcaacctgga accgcagcgt tgccgtagat tgcggggcga ctgtcacgcc
     6961 ctgggttcgc ggtgtgatcg agaccagaga atagtcgcca gcgtcccggg cgggccaggc
     7021 ttccaggggc gccaccagcg ccacctccgc atcgatgtct tcgcaggccg gattcgtcag
     7081 gcgcacgacg atatcgccgc cctcgtgggc cgcgtccatg gtgagctcga acgcctcgcc
     7141 gatctcgagg acatcctgga aggtctgtcc gtcaaattca tagcgaagct tcacctgccc
     7201 cggcgcatcg gcttccgggc gcgtgatctg cacttctttc gtgccgtgtc ccagggcagg
     7261 cagttcgaac gccagcgcgc ccggcgacgc ctgccagcct tgaggcacgg cgacgtttgc
     7321 cgtcaccgcg atggctgcgt cggtatagtc gttgaccgcg ttgaccatga cccgcagcac
     7381 gcgcccgtgg tcttcttcgc gtacctcgcg gccgagggtg caggtgaccg cgccgtatcc
     7441 catcgggaga gactccgcat cgtgctccca ggaacgcacc cagaccggct gcacgggctc
     7501 ggcctgggtt ccgagtgtgc gccggcccgc tttgggtccg agcggattgg gcacgaaccc
     7561 gaccgtctcg atcgaaaacg gcggtacggg cacgtccagc ccgttttcgc ggatggggag
     7621 agggcgcgat tcctcttcca gcagattcgc catccaggcg tcgctcatgc cggccgcaaa
     7681 cgtgatccgg gccgtgctgt cgacgccctc cgtgtcgtaa aggcgcaatg taatgccgtc
     7741 ggccgggttc gacgccaccg tgcgctggaa cgcaggcagc gggttgccgg ccggtttcat
     7801 ggctgcgaga atgacgttcg agggcgatac ggtcaggaag gcctgttcag gcggaaggat
     7861 ggcttttgcc gccggtttga cctcccgggc gaggagcggg aaattgaact cgtgcgcggc
     7921 gtgctgggtg ttggctttgc gccagtcgcc gctgtgggga tagagcgcat agcgaaacac
     7981 gtgatttttg ttttccggca caagatagcc ctcggggaag gtggcagtgc tgccgtacca
     8041 gcggcaggtg tggcacagca tcatgcacag ggtgccattc cgttcgatgc tgttggcgta
     8101 cgtcccttca ttcagcaggg ccacgccgta ttcatcgacc acgcctttcc atcccgtcgc
     8161 gtcgcatttc ccggggatgc gcagcaccgc ggcgtcacgc agatcgctta gaagacggtc
     8221 gcacgcgctg gcgaggtctt ttggcgtctt tgcctcaatg acgagtaccg gcatcggccc
     8281 ccacgagagg tcttccagct cggaatcgct ggtgaacagg taggcgaatc cttcttcgtt
     8341 gagttgtctc tggaattccg cgacggtttt ggcgccctgc ctgttgagca ggtggttgga
     8401 atactggttg ttgtggtgaa cacccagcga gatgcggaac cgcgtgtaca gcaggtcgtc
     8461 gtccatgtgg tcctggtatg agccccagta cggtccgccc gtgtcgtgcc agggcgtgca
     8521 ggtcacgccg cgctggatca acgcgcgctg gagtccttca gcgatgccgg tgatctcgct
     8581 ggtttttggc gtgatcagcc caaccatgct taccggataa gcgttccgtc cggccttgag
     8641 ctgcaccgag gggccatagt ccatccattt gttggcggcg tacacagcgc aaccactgaa
     8701 catcgccatc tgatgcgtgc ggaaatcgag gtagccccgg ctgctgcccc ggctgactgc
     8761 gccgaaccgt tcgtcgaaca ccggcgtagc gccggcaagg ttcacgggga acgtcacgca
     8821 gaacaggtga tcctcgccct gataatcctc cagaatggtc tggaaatcaa tccgggcgac
     8881 gcctttggtc agcgtgatgt cctgggtcac tccgcagagt tcgcccattc ggtacgtgac
     8941 gcgcagcgtc tcgctgacgg gcccgcggat ggccgttacg gatgccggaa tctctccgga
     9001 gaacagtttc aggcccgtgg tgtaaaattc gtgctgggtt tcctcccgat ccgcaatttc
     9061 ctggagggcc gccagttcat tgcccacatg cgcgccctgc tgcagcactt cgcgcttcgc
     9121 ttttttgtcg tagatgctga ccaggccgcc tccgcgcgcc ggatcgaccg tgatccggta
     9181 aaatttgttt tcgatcgacg tgcggcccgc cgtggttttg cgttctttgg gctctttttc
     9241 actgggcgtc acctgatatc ggcgataccc gagagagggc atgtctttgg ccacaaacgc
     9301 cacgcgcgct tcggtgatgc cgccgctccg gttgcgggcc gcgtcgagca gctcgaaagg
     9361 aacctcccgg ccgcggtggt cggtgatcgc aaactgcgtg aggccgccca cgtggatctg
     9421 cgcacggacc acgtccgtcc gttcccaggc cagtgggttg aacacgacca gcggctgatc
     9481 gtcaccccgc ggcgccacgg ccttgccgag gtagtgcagg ctgcgatcga gcacgtccga
     9541 gccgagttcc agcgcttccc ggtaggagtt catcaggtca atgtaggaaa gctcgttgtg
     9601 cgtaccggta atggaatcgt ggtggtggcc gcacaggagc tgccgccagg ccttgtcgag
     9661 cgccttgtcc gggtagacag cgcccagcag gccggcgagc gttgcgaacc gctccgccat
     9721 caaaagcacg ttctcaccca gacggttcgc gatcttcagg ttgacgcgcg acacggcggt
     9781 ggccgcgtgg tacagcgaca tatcgcgcga cgtcaccggc aggcaggcgc cgctttcctc
     9841 gcattgccgc acgacgatgt cgaagaggtc gcggtacgtc gcctgctgta ccttggggag
     9901 gagggtctcg tgccagtccg tcggcggggt ttggtcgatg tcgcccacga tgaccgacaa
     9961 gcccatgtcc gcggcttcgc tccatcccca gttgccgcgt ttgtggggca gtgaggaccc
    10021 gtccagcgcc agatgctgga acaggggcgg gaagttatag atgtatttgt cccagcttac
    10081 gcccaggcac ccggcttttc gcgcgatctg cgacaactgg ttcggatgtc cgaacacgtc
    10141 gcccggggcg tacacgtggc agatgcggcc caggacccgt ccgtggaaca gctggccata
    10201 gagaaaatta cgtaccagtg cctcaccgcc gcagttcatc gcatggggct ggttgtacat
    10261 cgcatcggaa ttcgagcggt tctcgcggaa tgcccgtatc aacgtctcgc gatcgtccgg
    10321 aaacacgtca aaatacggct tgagatagtc cacttccgac aaggtcgacc ggaactgcgg
    10381 gtcggtgcgg tgcaggcggc agtactggcg gacgatatcg aatgcgccca tcgcatagac
    10441 ccgctgttct tcgtggtagg tcgtgtcaaa atggaatgag gtcagcacca tctcgacgcc
    10501 ggccccctcg ggaggcgcgg ctgcctcgta ttgaaacggg gccgcaagct cccgcccgta
    10561 gagccgcgcc gtgaccgtcg cgtctacggt ggttccctcc ggcacggcgg gcagcgacag
    10621 ccgcagcgtc gtaatgcggc ccggcggcaa tacggcgttg atcttgtctt cggcggtggg
    10681 cgcgcttgat acgcaggtga aagtggcccg tacatccacg cgggacgtgt tgatcgcggc
    10741 cacttcgatg agttgccgcg gctgttcggg cgtgcccgtg aaccggggca atgcacggac
    10801 ccggcccagg gcgatgggca ggccccgctc gctcaccaac ggcgacaggc gcacatcccg
    10861 cacccggaaa tcgatcccgt cggcgatata tcccggccgg aacttgatca gcaggagatt
    10921 taaacctgcc ttcaacgaca cacgcgtgcc gggccgggtc agccgcaccc ctttgggaaa
    10981 accatacggc tcattgcaga ccagctggcc attgagccac gctttcattc cggagtgctg
    11041 cgcgtcgagc agggcgcttg tgtctgtggc ggcctcaata aacgcggcgg catagatcac
    11101 gcaattgcgc tgcaccgtct cgtagatcgc ttctcccgcg atgcgctccc cgtcgagctc
    11161 cgcgccctca cacgcgctcc aacgcaattg tgccgggccc aggcccgggt tccggtggac
    11221 gcgccccggc tccggccgta ccgccgtttc gccgccgtcg tccgcaagat agtcgatgtc
    11281 gaggatacgt tcgcgctcgt acagatactc gcgctcgaac tgcgggctgg tctggaccac
    11341 gaagggtccc agcaccagcc agcgagtcac aggatgaccc gccgcaagtt ccgcgagggg
    11401 atggatgttc attgtcgcgc tcctttatct cgcgccggca gccccgtgaa gacggcagcc
    11461 ggaactcaaa gccagcggaa aatgtcctga atctgcgccg caggcccgcg ttgctctgtg
    11521 gcaatcgttg cgccggtaca cccgcttccc ggtgcgttcc ccatgacttc agcttagcct
    11581 tttgatggag ggctgctcaa gcggtccccg ctgtcacgcc acacaacaac aggatcatct
    11641 ggctccatgc ggcgccgcgc cgtttttcag ggtgtctttg atgattatct cgatggaacg
    11701 ggcgccgatc ccgggcaggc tgcgccggtt gatgaaaatg gtggggatac tttggacttg
    11761 tagctgtatt cccagctcaa tatcctggcg tacggccgcg cgcacctcct ctgacgcccg
    11821 ctccgcgttg aaccgagcca aatccaggcc gatttcagcc gctacggctt ccaacgcctc
    11881 cggagtcgga ggaaaatgcg ttttgaacag cgtatcgtgg aattcccaga atttgccctg
    11941 ccgctgggcc gcggctgccg cgaaggccgt ctcgcacgcg ccgctttcgc cggcttcacc
    12001 gcccgcgctg ggattgcagg cgccgtccag cgggaaatgg cggaaaacca gcgagatcgc
    12061 gcccgggttg ctgtccatca gcaccctcat ttcgcggctg aactgacggc aggccgggca
    12121 atgaaagctg ctgaacacca ccatttccac tggagcgtgt tccgatccgc gccgcggatt
    12181 ggccggactg acgggcagtt cgatttccgg cgcgtcggca taggccgcca acagcctgtg
    12241 cggactgaaa acgtcccgtg attcacagag ccagcgctcg gagagaataa acaggccctc
    12301 atacaacgcc agcgcggtca gggcggccgt gaacagtccc gtcgcccgcc agcgccacag
    12361 gggcggccgg ccggcaggtt tctggaaaag cgggcgcagc gccgaggcga gagctttggc
    12421 cactccggcc cagctggagc cgaggcgtat cttcagcgct gcggcgagga gcaggttgat
    12481 cacgtgcacg accatgcaca gggggcagaa gggcgcccag cctcccagca ggagcgtcgc
    12541 gagcacgagg ctgctcattg cgcccgctac cgacatcatg agggcggccg tcagcgactg
    12601 cggagcgaac tcgtcgccca gggcgcgagc cataaaaaac agcataagga gcgcgccgta
    12661 gtagacaatg ccccatcccg ccaggggaag gcccagcacc cacgacgtgg cgctgagcag
    12721 ggtcttgtca caaccagcgc cgaaaatcgc cgagcatacg tcgaacgcat cggggtcctg
    12781 gctcatcagg agggtcagcg tacgcccaaa gagatagccc gacaccagca ggcccaggag
    12841 cgtgagtgcc accggggcgc tccgtgcggc tgtgcgcatt ctaccgcctg tcatccaatt
    12901 ggcctcctca cctggcttcg gcgggatgca ccccggggga atacgcccgc acggggccgg
    12961 ggcgccgtgc gggcagccgg gcactgatgg ctacagcgat acggcggcgc cggttcgggc
    13021 ggactcatag catgcggcca gcacatactg gctccagagg ccatcttcgg cggataccgg
    13081 gggttccgtg ccttcaagca cagctcgcga gaaggcttcg acctctgcac ggtacgtgtt
    13141 aaccggttcc ggcgtgatgg cgatgccgcc gccggcggtg cgggcctgct gcgcctcgta
    13201 tccggtgtcg cggctttcga gatacgcg
//
LOCUS       DAOPBQ010000029        13148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4708_length_13148_cov_4.298480, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000029 DAOPBQ010000000
VERSION     DAOPBQ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4708_length_13148_cov_4.298480"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="PKW60_01950"
     CDS             <1..1357
                     /locus_tag="PKW60_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPJ98224.1"
                     /translation="VDTVVYCTWSSGFGLFTHNTKVGEIFTSTEPAPNGEKGFSWNQT
                     KAFLEQGTDPLEIMVNFCRQNGIEVFWSMRMNDCHDGWGDWYSPIMFPQLKKDHPEWL
                     MGTAEKRPVNGPWTAVDYGQPEIRDLAFKFFEEVCANYDIDGIQMDFFRHLAYFRAHA
                     EGRPCGQAELDMMSDLMRRVRVMADETGAKRGRPILISVRVPDSLEMCRDMGFDLERW
                     MRDDLIDILTLSCYFRLNPWKTSVELAHKYNVPVYPSLSETRLRDKDARSERANLEAY
                     RARAMNAWHAGADGIYMFNFFNPNAPHWRELGDPDVLAPLDKVYTTGARGYGNLSFWY
                     KGGEKYMNRDILNPDYPRTLAAVAPVSITLPVGEDVTAHAPVTVTLRLRFKEAPAAKV
                     IAVTLNGAPLTAETQDGPWLEFPVEPALVKQGDNVFALGVAAGEPTRPVLLDLLLRVR
                     H"
     gene            complement(1407..2789)
                     /gene="lpdA"
                     /locus_tag="PKW60_01955"
     CDS             complement(1407..2789)
                     /gene="lpdA"
                     /locus_tag="PKW60_01955"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPJ98225.1"
                     /translation="MVTHDLIVIGAGPGGYVAAIRAAQLGLNVACVESEAALGGTCLR
                     VGCIPSKALLESSARFVEVRDTLSKHGIQVTGLSLDLAGMMRRKEDIVKGLTNGVAGL
                     FKKNGITRYEGRGCITGPGRITVEGKEDVALAAQHIIIATGSRSAPLPNVEVDGLYVG
                     TSTDALAYDVVPGHLVVIGAGYIGLELGSVWNRLGAKVTVLEYLDRILPGMDSELAAK
                     AQKIFEKQGMTFRLGCRVTGARKKGKGAVVDIDGADPVACDRVLLSVGRVPNTEELGL
                     ETMGIETDSRGRIVVDEHFRTSAGGFYAIGDVIPGPMLAHKAEEEGVACVENLVKGYG
                     EVNYNAIPGIVYTHPEIASVGKTEDQLQEEGVPYRKGVFPYLANGRARCIGDTEGFVK
                     MLAHAETDRLLGVHILGTQAGDLIAEAAVALEFDASSEDLARSTHAHPTLAEIMKEAA
                     LAVDGRAIHI"
     gene            complement(2859..4070)
                     /gene="odhB"
                     /locus_tag="PKW60_01960"
     CDS             complement(2859..4070)
                     /gene="odhB"
                     /locus_tag="PKW60_01960"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HPJ98226.1"
                     /translation="MATDVQVPALGESISEVQIARWLKAPGEYVKQDEPVVEIDSDKA
                     TLEVPAPVAGLLAEVLVNEGENASVGDVIARIDETAPVPAPVEGPRPPAAVEESPPRA
                     DEKAVVKKEQRVMPAAKRALAERGLDPNAVKPTGRGGRVLKEDVLQHSEKAPAPEPAA
                     SPVGGIVTGAQRREKLRPITPMRRRIAERLVQAQQTAALLTTFNEIDMSAVIALRKEH
                     QESFVAKHGVKLGFMSFFVKAVVTALREFPGVNAQFRDDQLVEFNYYDIGIAISSGKG
                     LVVPVLRNAELMRFADIERAIADFGGRARDNKIALEELQGGTFTITNGGVFGSLLSTP
                     IVNPPQTAVLGMHAIQERPVAIGGEVVIRPMMYVALTYDHRMVDGREAVTFLVRVKEC
                     VENPARLLLDL"
     gene            complement(4130..6973)
                     /locus_tag="PKW60_01965"
     CDS             complement(4130..6973)
                     /locus_tag="PKW60_01965"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HPJ98227.1"
                     /translation="MANPETELSSLSLEFMEGLYEQYLDQPDSIPADWREYFRRLSDN
                     GDFTPAPAVPQDTCVRRRWFQSDWEEAIKCQICGRDINLAAMQHKVDKLVRNYRVRGH
                     RVAQVDPLRSPVPELEELDPAYFGFSDHDMDLPFSTTTLTEEDTLSLAEIIERLRNTY
                     CRSIGVQFMHIDDLDLREWLQERMERTENRIALSRNEQIRILTRLTDAVIFEEFIQKK
                     YVGAKRFSLEGAESLIPLLDMAIEKAGQYKIDEIVIGMAHRGRLNVLTNILGKSPGRI
                     FREFDDEDPELHIGRGDVKYHLGHHSDWTTEAGHTLHLALCFNPSHLEFVNPVAMGRV
                     RAKQDRLGDVRREGGMCLLIHGDAAFAGEGIVQESLNLSQLEGYRIGGTIHVVVNNQI
                     GFTTVPSEGRSSVYATDVAKMLQIPIFHVNGEDPEAVAQVIDLAMEFRRKFQRDVVID
                     MYCYRRHGHNETDEPAFTQPLLYAKINRRRSVREGYLEHLLKLGGISREEADEIAERR
                     RAVLEKEFTASRSKEEEQAREERKRNPKRKSVLGVLWSRYFGGPEKNAPNPDTGVARD
                     TLVSHLEALTVLPDGFHVHPKARRLLTARKAMAAGKQPLDWAAGEALAYASLAAEGVR
                     VRMSGQDSQRGTFSHRHAVLVDTVDGRAYMPLQHITPEQGPVDIVNSPLSEAGVLGFE
                     YGYSSAYPDGLVLWEAQFGDFANAAQVLIDQGITSAEDKWGALSGLVLLLPHGFEGMG
                     PEHSSARLERFLMLGAEDNIQVVYPTTPAQFFHCLRRQVHRPWRKPLIVMTPKSLLRH
                     PKVVSPLEDLARGRFRHILPDEMGIPGDRVKRVLLCSGKIYYELVEERAARGRDDVAI
                     VRVEQLFPLHEEYLEEVLRDYPAETDMVWVQEEPQNMGAWWLWRVRFCDRLLGRWPLR
                     GVYRAPSASPATGSAASHRLEQRLLLDEAFADL"
     gene            complement(7472..8038)
                     /locus_tag="PKW60_01970"
     CDS             complement(7472..8038)
                     /locus_tag="PKW60_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPJ98228.1"
                     /translation="MVAVPVARDIMKTTIRVVHPDMSVLAAMHTLHSKGENAAMVVDD
                     DFRLVGILTEKDCLRVLLGEMYDRFQEIGRALVADFMSPVVETLTPGMDLFTIATVFL
                     RTNFTALPVLDEEILTGAVYRLSMLAAIVDMVKRAEAGHALFLEKIKMTEYPQSIQDL
                     LRLAGGHSRQQLKEVFSRRHCPPPEDLC"
     gene            8292..9062
                     /locus_tag="PKW60_01975"
     CDS             8292..9062
                     /locus_tag="PKW60_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HPJ98229.1"
                     /translation="MSELGKATRLNRILAGRTRRVLTVAVDHMINYPEKFPLELRRMA
                     STLEQVIAGDPDAVTMNKGPALRLMAPYAGRVPLIIQSMALRPHEPSFADTATVEETL
                     GLGADAIAVAMFVYGETELDYLRHLAEVVRCAAPFGLPVIPHIYPLESGDEKHAIVHD
                     PEHVHYAARVGFEMGADVVKVPYTGDVASFRDIVADIPVPVVSAGGPRCDSFGDAVAL
                     ARDVGETGAAGATMGRNVWGFPDIPKAIRRLKEAINEA"
     gene            9193..10041
                     /locus_tag="PKW60_01980"
     CDS             9193..10041
                     /locus_tag="PKW60_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98230.1"
                     /translation="MATTVTVPTWFYQHGDADFTLDVPEEAFGGWKRANLDLSLDNTA
                     VVVMHAWDAGTSPDQFPGWWRVVPYIVRANAIMETTFPPLLEAVRRSPMPLFHVVAGG
                     DYYKHLPGYERAAELAPRQENTVEKIAPDPVLQTALGYKNEHGYPRPHNVPDIQRGFS
                     RIDFGPHAHPQGDEGVAENGPQLYGLCRAHNITHLIYMGFAINWCLLLSPGGMAEMTY
                     QYGVTCSAFRQATAAVENKASARDQWCKELALWRVALAFGFVYDVDDFITALKTSGSA
                     TGTAQP"
     gene            10038..10745
                     /locus_tag="PKW60_01985"
     CDS             10038..10745
                     /locus_tag="PKW60_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPJ98231.1"
                     /translation="MTTSGPLSGKRAVVTGASRGIGRHIARALCDAGATVAITGRNRE
                     TLEAVARELGPGCLPQICDQRDPEAISAMAAAVINALGVPDILVNNAGLFRGGPVVNM
                     ALADWNEVIETNLTGVFLTTQAFLPGMLERKRGDIFIISSMSGKKGDPNAAAYAASKF
                     GLQGFAQALLYEVRKSNVRVMVLNPSSVDTSVDHGRKEGSGLHLHAADLAATVVHLAA
                     LPGRTLFRDMDIWGTNP"
     gene            10841..11443
                     /locus_tag="PKW60_01990"
     CDS             10841..11443
                     /locus_tag="PKW60_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98232.1"
                     /translation="MKRKTSIIGALGLALAIVVCGCETVPRGAALGGALGAGTGAIIG
                     HQSGHAGEGAVIGGLVGAAAGAVASDVRARRTRTPEETAKAYDYQPAQGEMLKLENVS
                     VLPTQIRRGDMLEASIQYALLGTPSGGTTVRESRSLMQGGRVVAEIGTKSFTREDGTW
                     VSAVQFKALDSLEPGDVIVQQVIETASSRVMGTDKFTIIE"
     gene            complement(11470..12177)
                     /locus_tag="PKW60_01995"
     CDS             complement(11470..12177)
                     /locus_tag="PKW60_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98233.1"
                     /translation="MWWAAIAAALILASGCDGPRRGRLVTPPEAMSPSAASEMPADAA
                     SAMPGAGAVFALAVPGRGVLTLAVPAETAAAQAESSVPGGAAVRLEGTGPEPYVTILT
                     VMGVPGMMPGFGADAWFAEELERWKDGLSETERAASSVPVEFQLDRVHGVYLNLEDPA
                     PQPGHYPYLTQGFFDVDGCVVALQTLHHSSRASAVNRYLAVLKDAVWEPESVPLPDAG
                     PPPAPEKETPPDTPAAQ"
     gene            12489..>13148
                     /locus_tag="PKW60_02000"
     CDS             12489..>13148
                     /locus_tag="PKW60_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98234.1"
                     /translation="MSISRRKFLGTTASAVIVAGTMAKGRVFGANDRVNVCVMGINGR
                     GNSHIKGFNSIRQARVAGLCEVDTTLFDSRVEEYFKRANLSVPKTYADIREVLDDKDI
                     DAVSCAAPNHWHSLATVWACQAGKDMYVEKPMTHNIFEGLKVVEAAKKYNRIVQHGTQ
                     LRSNPGFQEGIQLLKDGFIGDVYMARCVCYKWRGDIGKAVPGQPPSTLRWDLWQGPAK
                     EK"
BASE COUNT         2516 a         4137 c         3982 g         2513 t
ORIGIN      
        1 ggtggacacg gtggtctatt gcacctggag ttccggattc ggcctgttca cgcacaatac
       61 aaaggtgggc gagatcttca ccagcaccga gcccgcgccg aacggcgaaa aaggcttcag
      121 ctggaaccag acgaaggcat ttctggaaca gggcacggat ccgctcgaga tcatggtcaa
      181 tttctgccgc cagaacggga tcgaagtgtt ctggtccatg cgcatgaacg attgccacga
      241 cggctggggc gactggtact cgccgatcat gttccctcaa ctcaagaaag accaccccga
      301 atggctgatg ggcacggcgg aaaaacggcc tgtgaacgga ccctggaccg ccgtggatta
      361 cggccagccg gagatccgcg atctggcgtt caaatttttc gaggaagtct gcgccaacta
      421 cgacatcgac ggcatacaga tggacttttt ccgccatctg gcgtatttcc gcgcacatgc
      481 cgaagggcgg ccctgcggcc aggccgagct ggatatgatg tcggacctga tgcggcgcgt
      541 ccgcgtgatg gccgacgaga ccggcgcgaa acgtggacgg ccgattctca tctcggtgcg
      601 cgtgccggat tccctggaga tgtgccgcga catgggtttc gatctggagc gatggatgcg
      661 ggatgatttg atcgatatcc tcaccttgag ctgctatttc cggctcaatc cctggaaaac
      721 cagcgtcgag ctcgcccaca aatacaacgt cccggtctat ccctcgctga gcgagacccg
      781 gttgcgcgac aaggatgccc ggtcagagcg ggcgaacctc gaagcctacc gggcgcgcgc
      841 catgaacgcc tggcacgcgg gtgcggatgg catctacatg ttcaatttct tcaaccccaa
      901 cgcaccgcac tggcgcgaac tgggcgaccc ggacgtcctg gcgcccctcg acaaggttta
      961 caccacgggc gcccggggct acggcaatct cagcttttgg tacaagggcg gcgagaaata
     1021 catgaaccgc gacatcctca accctgacta cccgcgtact ctggccgccg tcgcccctgt
     1081 ttcaatcacc ctgccggtgg gggaagatgt tacggctcat gcacccgtga ccgtgacgct
     1141 gcgcttgcgg ttcaaggaag ccccggcggc caaagtgatc gccgtgacgc tcaacggcgc
     1201 cccgttgacg gcggaaacgc aggacggccc gtggctcgag tttcccgtcg agcccgcgct
     1261 ggtcaaacag ggcgacaacg tctttgccct gggcgtggca gcgggcgagc ccacccggcc
     1321 cgtattgctc gatctgctcc tccgcgtacg ccactgacga atgttcaccc ggcgtcagga
     1381 gggcacggcg ccgccgcctc aaagcgtcag atgtgaatag cgcgcccgtc gacggccagc
     1441 gccgcctctt tcatgatctc ggccagggtg gggtgcgcgt gggtcgaacg ggcgaggtct
     1501 tcgctgctcg catcgaattc cagggccacg gcggcttcgg cgatcaggtc gccggcctgc
     1561 gtgccgagga tgtgcacgcc caggagccgg tcggtttcgg catgggcaag cattttgacg
     1621 aaaccctctg tgtcgccgat gcaacgcgca cggccgttgg cgagataggg gaacacgccc
     1681 ttccggtagg gcacgccctc ttcctggagc tggtcctccg ttttgcccac cgacgcaatc
     1741 tccgggtgcg tatacacgat tccagggata gcgttgtaat tgacttcgcc gtagcctttc
     1801 accaggtttt cgacgcaggc cacgccttct tcttccgctt tgtgagcaag catgggaccg
     1861 ggaattacat cgcctatcgc atagaaccct ccggcggagg tccgaaaatg ttcgtcgacc
     1921 acgatgcggc cacggctgtc ggtttcaatc cccatcgtct ccagacccag ctcttcggta
     1981 ttgggaacac gccccacact gagcagcacg cggtcgcagg ccacaggatc cgctccgtcg
     2041 atgtcgacaa ccgcgccttt gcccttcttt cgcgcgcccg tgacccggca tcccagacgg
     2101 aaggtcattc cctgcttttc gaagattttc tgcgccttcg cggccagttc tgagtccatg
     2161 cccggcagga tccggtcgag atattcgaga acagtcacct tggcgcccag gcggttccag
     2221 accgacccta attccaggcc gatgtatccc gcaccgatga cgaccaggtg tccgggcacc
     2281 acatcgtacg ccagtgcatc cgtgctggtg ccgacataga gcccgtccac ctctacgttc
     2341 ggcaggggcg ctgatcggct gcccgtcgcg atgataatgt gttgcgcagc cagcgccaca
     2401 tcttctttgc cttcgacggt gatgcgtccc gggccggtga tgcacccgcg tccttcgtag
     2461 cgggtgatgc cattcttctt gaacagtccg gccacgccat tggtcaggcc tttgacgata
     2521 tcttctttgc gccgcatcat cccggccagg tcaagcgaga ggcccgtaac ctgtatcccg
     2581 tgtttcgaga gcgtatcgcg cacttcgaca aaccgcgcgc tggattccag caatgccttg
     2641 ctgggaatgc agccaacgcg caggcaggtg ccgccgagag ccgcttcgct ttcgacgcag
     2701 gccacattca atcccaactg agcggcgcgg atcgcggcga catagccgcc cgggcccgcc
     2761 ccgataacaa tcagatcatg cgtaaccagg ttcatggggt ctccttccgt tgtcacgtgg
     2821 ttgcctggcg ccgccggccc gtccggcctg ctcgcgcact ataaatccag gagcaggcgc
     2881 gcaggattct caacacattc tttgacccgc acgagaaacg tcacggcttc ccgcccatcg
     2941 accatgcggt ggtcataggt cagcgcgaca tacatcatgg gccggatcac gacctcgccg
     3001 ccgattgcca ccggccgttc ctggatggcg tgcatcccca gcacggcggt ctgcggcgga
     3061 ttcacaatgg gcgtggacag aagcgagccg aacaccccgc cgttcgtgat ggtgaacgtc
     3121 ccgccctgca gctcctccag cgcgatcttg ttgtcgcgtg ctcggccgcc gaaatccgcg
     3181 atggcgcgct cgatatcggc aaagcgcatc agctcggcgt tgcgcagaac cggcaccacc
     3241 aggcctttac cgctgctgat ggcgatgccg atgtcgtaat agttgaattc gacgagctga
     3301 tcatcgcgga actgcgcgtt gacaccggga aattcccgga gcgccgtgac cacggctttc
     3361 acgaaaaacg acatgaatcc gagcttcacg ccgtgcttcg ccacgaacga ctcctgatgt
     3421 tcctttcgca gcgcgatcac tgcggacatg tcaatctcgt tgaacgtggt gagcaacgcg
     3481 gccgtctgct gcgcctgtac gagccgttcc gcgatgcggc ggcgcatcgg cgtgatcggg
     3541 cgcaactttt cgcggcgctg cgctccggtg acaattcctc cgaccggcga cgccgcgggc
     3601 tcgggcgctg gcgctttttc agaatgctgg aggacatctt ccttcaggac gcgcccgccc
     3661 cgccctgtcg gtttgactgc attggggtcg agcccgcgct ccgccagggc gcgtttcgcc
     3721 gcaggcatca cacgctgctc ttttttcacc acggcctttt catcggcgcg cggcgggctc
     3781 tcctcgactg ctgcaggcgg gcggggccct tcgaccgggg ccggaactgg cgcggtttcg
     3841 tcgatgcggg cgatgacgtc tcccacgctg gcgttctcgc cttcgttgac gagcacttca
     3901 gccaggaggc ccgccacggg ggcgggaact tcgagagtgg ccttgtcgga atcgatttcg
     3961 acaacgggct cgtcctgttt tacgtattct cccggagcct tcagccagcg ggcaatctga
     4021 acctccgaga tggattcacc cagcgctgga acctgcacgt cagtcgccat aaaactactt
     4081 ctccttcatc gcgctgctgc tgctgtatgc cacaggcccg tccgcgtcat catagatccg
     4141 cgaaggcctc atcaaggagc aggcgctgtt caagccggtg gctcgcggcc gagccggtcg
     4201 cgggactcgc tgaaggcgcg cgatacacgc cgcgcaacgg ccaacggccg agcagccggt
     4261 cgcagaagcg gacacgccac agccaccacg cccccatatt ctgcggttct tcctgcaccc
     4321 agaccatatc cgtctctgcg ggatagtccc gcaggacttc ttcgaggtat tcctcgtgca
     4381 ggggaaacaa ttgctcaacc cgcacgatag cgacgtcatc gcgcccgcgc gccgcgcgct
     4441 cctcgacgag ctcataatag attttgccgc tgcacaggag cacgcgtttc acccgatcgc
     4501 ctggaatacc catctcatcg ggcagaatgt gccgaaaccg gccccgcgcc agatcctcga
     4561 gcggcgacac gactttcgga tgccgcagca gacttttggg ggtcatcaca ataaggggtt
     4621 tccgccacgg ccggtgaacc tggcggcgca ggcaatgaaa aaattgcgcc ggtgtcgtcg
     4681 ggtacacgac ctggatgttg tcctccgcgc cgagcatcag gaaccgctcc aggcgcgcgc
     4741 tcgaatgctc ggggcccatt ccctcgaatc catgcggcag cagcaggacg agtcccgaga
     4801 gagcgcccca cttgtcttcg gcgctcgtga tgccctggtc aatcagtacc tgcgccgcat
     4861 tggcaaaatc gccaaactgg gcttcccaca gcacaagacc gtcgggatac gccgaactgt
     4921 agccgtattc aaaacccagg acgcccgctt ccgacaaagg gctgttgacg atgtcgaccg
     4981 gcccctgttc cggagtgata tgttgcagcg gcatgtaggc acgcccgtcg accgtatcca
     5041 ccagtacagc gtgccggtgg ctgaacgtgc cgcgctgcga gtcctggccg ctcatgcgca
     5101 cgcgcacacc ctcggcagcc agggaggcgt aggccagtgc ctcgccggcg gcccagtcga
     5161 gcggctgctt gcccgcggcc atggccttac gcgcagtcag gaggcgccgg gccttgggat
     5221 gtacgtgaaa accgtcggga agcacggtca gcgcctcgag atgcgacacg agcgtgtctc
     5281 gcgcgacccc cgtgtcgggg ttgggcgcgt ttttctcggg accgccaaaa tatcgagacc
     5341 agaggacgcc cagcacgctc ttgcgtttgg ggttgcgttt gcgttcctcg cgcgcctgtt
     5401 cttcttcctt gcttcgggaa gccgtgaact ctttttcaag aaccgcgcgc cggcgttctg
     5461 cgatctcatc ggcctcttcc cgggagatcc cgccgagttt cagcaggtgt tccagatagc
     5521 cttcacgcac ggagcggcgc cggttgattt tcgcgtaaag cagcggctgg gtgaatgcag
     5581 gctcatcggt ttcgttgtgg ccatgcctgc ggtagcaata catgtcgatc acaacatccc
     5641 gctggaattt ccgccgaaat tccatcgcca ggtcaatcac ctgggcgacc gcttccggat
     5701 cttcgccgtt gacgtgaaaa atcgggatct gaagcatctt ggcgacgtcc gtcgcgtaga
     5761 cgctcgagcg cccttcggaa ggcacggtcg tgaacccgat ctgattgttc accacgacat
     5821 ggatcgtgcc tccgatacgg tatccttcga gttgggacag attcagcgat tcctgcacga
     5881 tgccttcgcc tgcgaacgcg gcgtcgccat gaatcaggag gcacatcccc ccttcgcggc
     5941 gcacgtcgcc gaggcggtcc tgcttggcgc gcacccggcc catggcgaca gggttcacga
     6001 attccaggtg gctggggtta aagcacagtg ccagatgaag cgtgtggccc gcttccgtgg
     6061 tccagtcgga atggtggccg agatggtatt tgacgtcgcc acggcctatg tggagttccg
     6121 ggtcttcgtc atcaaattcg cggaaaatgc ggccgggact cttcccgagg atattggtga
     6181 ggacgttgag gcggccccgg tgcgccatgc cgatgacgat ctcgtcgatc ttgtactggc
     6241 cggccttctc gatggccata tcgagcaagg ggatgaggct ttccgctcct tcgagtgaaa
     6301 aacgcttggc gcccacgtat ttcttctgga tgaattcctc gaaaatgacc gcgtccgtga
     6361 gccgcgtcaa aatgcggatc tgctcgttgc ggctgagcgc aatgcggttt tcggtccgct
     6421 ccatacgctc ctgcagccat tcgcggagat ccaagtcatc gatatgcatg aactgcacgc
     6481 cgatagaccg gcagtaggtg ttgcgcagcc gctcgatgat ttcggcgagg ctcagggtgt
     6541 cttcttccgt cagggtggtt gtggaaaacg gcagatccat gtcatggtcg gaaaatccga
     6601 aataggccgg gtcgagttct tccagttccg gtaccggcga cctcagggga tccacctgcg
     6661 cgacacggtg cccgcggacc cggtagttgc ggacaagctt atccacctta tgctgcatcg
     6721 ctgccagatt gatgtcgcgt ccgcagatct ggcatttgat ggcctcttcc cagtcggatt
     6781 gaaaccatcg gcgtcgcacg caggtatcct ggggcacggc aggcgccggg gtaaaatctc
     6841 cattatccga gaggcgcctg aaatactccc gccaatccgc tggaatcgaa tccggttggt
     6901 cgagatactg ctcgtagagg ccctccatga actccagact caagctgctc agttcggttt
     6961 caggattggc catgtcttct ccgcctttgc cgcccctggg ccgcacagcc cggatgggcg
     7021 caccgtgccc tgtcttcgtt gttccgatcg cacagactgt ggattgaccg ccccctcccg
     7081 ccacggacgc cgtttctgcc ggaaacgccg ccggtattca aggcaactcg atgtccgcag
     7141 cagccttacc acgaaacccc catcgccacc ctgccttaaa cagagcgaaa accgtgcctt
     7201 acgccaccat aggcagaacc attgagagtc gagaaatgtt tcttgggaca cactttagcg
     7261 cattttcgag aagtttgctc aagaagaact gtgcaatctg cgatttctgc acatcctgcc
     7321 tcccatggca ggcaggcggt ggttcgagca gcacgctcac tggcgggtag aggccgagcg
     7381 aaccgagcag cgcaccgtcg agcccgggcg tcgaattgtt ctgcgctgcc ttgccgcccg
     7441 gcagcggcag ggcggtacgt gccggcgggc ctcagcacag atcttcaggc ggcggacaat
     7501 gccggcggct gaacacctct ttcagctgct gacggctgtg gccgcccgcg aggcgcagca
     7561 gatcctgtat tgattgcgga tattccgtca ttttgatctt ttcgagaaac agggcgtgtc
     7621 cggcttctgc gcgtttgacc atgtctacga tcgccgccag catgctgaga cgatacacgg
     7681 cgccggtcag aatttcttca tccagcacgg ggagcgccgt gaaattcgtc cggagaaaca
     7741 cggttgcgat agtaaacagg tccatccccg gtgtcagtgt ttcgactaca ggagacatga
     7801 aatcggccac cagcgcgcgg ccaatctctt gaaaccggtc gtacatttca ccgagcagaa
     7861 cgcgcaggca gtccttttcc gtcaaaatgc ccaccagccg gaaatcatca tccacaacca
     7921 tggcggcgtt ttcgcctttg ctgtggagtg tgtgcatcgc ggccaagacg ctcatgtcgg
     7981 gatgcacgac gcggatcgtc gttttcatga tgtcgcgtgc tacaggtact gccaccatgg
     8041 ctccctcctt ctggctttgc ccagagggtc cgtgtgcgct gttgactgta ttcgcgtgct
     8101 ggctttcagt ataacacgga aaccgcccgg gagcggggtt ctgttcaagg caagccccct
     8161 ggatctgccc ggcagatacg ttggcaactc ctttcaaaca aaagatatta atccgttttg
     8221 agaatgggcc gcaggtgccg taccataggc ccgtttcagg cgaataagtc aaagaaaggc
     8281 agacagatac cgtgagcgaa ctgggaaaag cgacccgact gaaccgcatt ctcgccggaa
     8341 gaacgcggcg cgtacttacc gtcgcggtgg accacatgat caactatccg gagaaattcc
     8401 ccctggaact gcgccggatg gcctcgacgc tcgaacaggt tatcgcggga gatccggatg
     8461 cggtaaccat gaacaaaggc cccgcactcc gcctcatggc gccgtacgcg gggcgcgtac
     8521 ccctgatcat ccagtcgatg gcgctgcgcc cgcatgaacc gagcttcgcg gacaccgcaa
     8581 ccgttgagga gaccctgggg ctgggggccg acgccatcgc cgtggccatg tttgtctacg
     8641 gagagacgga actggactac ctgcggcatc tggcggaggt ggtgcgctgc gcggcgccgt
     8701 tcgggctgcc ggtaatcccg cacatctatc ccctcgagag cggcgatgaa aaacatgcga
     8761 tcgtgcatga ccccgagcac gtgcactatg cagcgcgggt gggatttgaa atgggcgccg
     8821 acgtggtcaa ggtgccgtat acgggcgatg tcgcatcgtt tcgcgacatc gtggcggaca
     8881 tccccgtacc ggtggtctcg gccggcgggc cccgctgtga cagcttcggc gatgctgtcg
     8941 cacttgcccg tgacgttgga gagaccggcg ccgccggcgc caccatgggc cgcaatgtct
     9001 gggggtttcc tgacatcccc aaggccatcc gccgcctcaa agaggccatt aacgaggcgt
     9061 aacgcctcag ccgccggttt ccgcgggaaa gaccgcgcca gacaccgcgt attcggggcg
     9121 gttgcggcgt agatgccgga tgtttcatga tacagaggcc tccagaaacc caggttccag
     9181 gagatcaatg gaatggcaac aacggtgacg gtgccgacgt ggttttatca gcatggcgac
     9241 gcggatttca cgctcgatgt gcctgaggaa gcgtttggcg gttggaaaag ggcaaacctc
     9301 gatctgtcgc tggataacac ggcggtcgtg gtcatgcatg cctgggacgc cggcacctca
     9361 cctgaccagt tccccggctg gtggcgggtg gtgccgtaca ttgtgcgggc aaatgcgatc
     9421 atggagacga cgttcccgcc gctgctggag gcggttcgcc gctcacccat gccgctgttt
     9481 catgttgtag cgggaggcga ctattacaaa cacctgccgg gatacgagcg cgcggctgaa
     9541 ctggcgccgc gccaggaaaa cacggtagaa aagatcgcgc ccgacccggt cctgcagacg
     9601 gcgctcggct acaaaaacga gcacggatac ccccggccac acaacgtgcc agacattcaa
     9661 cggggttttt cccgcatcga tttcggtccg catgcccatc cccagggaga cgagggcgtg
     9721 gcggagaatg gcccgcaact ctatggtctg tgccgcgccc acaacatcac gcacctcatc
     9781 tacatggggt ttgcgatcaa ctggtgcctc ctgctgtctc ccggaggcat ggccgagatg
     9841 acctaccagt acggcgtcac ctgcagcgcg ttccggcagg ccacggcggc ggtcgagaac
     9901 aaagcctctg cccgcgacca gtggtgcaag gagctggcgc tgtggcgcgt ggcgctcgcg
     9961 ttcgggtttg tctacgatgt ggacgatttc atcactgcgt tgaaaaccag tggcagcgcc
    10021 accgggacgg cccagccatg acaacgagcg gtccgctcag cgggaaacgg gctgtagtga
    10081 ccggcgccag ccggggtatc ggccggcaca tcgcacgggc cctgtgtgac gccggagcaa
    10141 cggtcgccat caccggccgc aaccgggaga cgctcgaagc ggttgctcgt gaactgggac
    10201 ccggctgcct gcctcagatc tgtgatcagc gcgacccgga agccatttcg gcgatggctg
    10261 ccgctgtgat caatgccctg ggcgttcccg acatcctcgt caacaatgcg gggttatttc
    10321 gcggcggacc tgtcgtgaac atggccctgg ccgactggaa cgaagtcatc gaaacgaacc
    10381 tgacgggagt atttctgacc acccaggcgt tcctacccgg aatgctcgaa cggaagcgcg
    10441 gcgacatttt catcatcagc tcgatgagcg gtaaaaaagg cgacccgaac gcagcggctt
    10501 acgcggcatc caagtttggc ctccagggct tcgcccaggc cttgctctac gaggtccgga
    10561 aatccaatgt ccgggttatg gtgttgaatc ccagcagcgt ggataccagc gttgatcacg
    10621 ggcggaaaga aggctcaggc ctgcatttgc atgccgcgga cctggcggcc acggtcgtgc
    10681 atctggccgc cctgccgggg cgcactctgt ttcgcgatat ggacatatgg ggcaccaacc
    10741 cataggtttg gattaaatag aaatgcttgg cgtgcgtctg gattcaacgt ataatgagcg
    10801 tgattaagcg agagcataaa tctgtgaaag gatgcaaagg atgaagcgga aaacctcaat
    10861 tattggggcg cttgggcttg cgctcgccat agtggtttgc ggatgcgaga cagtgccgcg
    10921 cggggcagct cttggcggcg ccctgggtgc gggcaccggc gcgattatcg gccatcaatc
    10981 cgggcatgcg ggcgaaggtg cggtgatcgg cggcctggtt ggcgcggccg cgggcgccgt
    11041 cgccagtgac gtccgcgcac gccgcacccg gacgcccgaa gagacggcca aagcctacga
    11101 ttaccagccg gcacaaggcg agatgctcaa gctcgagaac gtcagcgtgc tgccgacgca
    11161 aatcaggcgg ggcgacatgc tcgaggcgtc gattcagtat gccctgctcg ggacaccttc
    11221 gggcggcaca accgttcgcg aatcgcgcag cctgatgcag ggcggacggg ttgttgccga
    11281 aatcggcacc aaaagcttca cgcgggaaga cggcacctgg gtgagcgccg tccagttcaa
    11341 agcgctcgac agcctcgaac ccggcgacgt catcgtgcag caggtcatcg aaaccgcctc
    11401 ctcgcgggtc atgggaacgg acaagttcac tatcatcgag taagaccctt cggggcgttc
    11461 aacggcgaat tactgagccg ccggcgtgtc cggcggcgtt tctttttccg gcgccggagg
    11521 agggccggca tccgggagcg gaacgctttc tggctcccaa accgcatctt tgagcacagc
    11581 cagataccgg tttacagccg aagcacggga ggaatggtgc agcgtctgaa gcgccacgac
    11641 gcagccgtcc acatcgaaaa atccctgcgt taagtacggg tagtggccgg gctggggcgc
    11701 gggatcttcc aggttgaggt acaccccgtg aacgcggtcg agctgaaact ctaccggaac
    11761 ggagctcgct gcccgctctg tctcggaaag gccgtctttc cagcgctcaa gctcttcggc
    11821 aaaccacgcg tcggctccga atcccggcat catgcccggc acccccatca cggtcagaat
    11881 ggtgacatag ggttccgggc ctgtgccttc cagacggacc gccgcgcctc cgggcacgct
    11941 cgattccgcc tgggctgccg ccgtttctgc gggaaccgcc agggtgagca cgccacggcc
    12001 cggcacggcc agcgcaaaga cggcgcctgc acccggcatc gcgctcgcgg cgtcagccgg
    12061 catctctgag gccgcactcg gggacatggc ttccggtggc gtgaccagcc tgccccgccg
    12121 gggaccgtca caacccgaag caaggattaa cgccgctgca atcgcggccc accacatgcg
    12181 ctttatcctc aatctcgcct ccccctgccg cggcggtgtc gagcggcaca ccacgttcac
    12241 cagacatccc ggtgcacagt tcccacttcc gtctccagaa atgtgacgca ttcgtgaaat
    12301 cattatcctg cgggttttcg aaaaacctat cttgcgcact cgcgtcggga attgcaacga
    12361 attcttcccg ttttcggttg caaattcaaa gaagaatgcg caattataaa acgttgtttg
    12421 tgagaatccg ggttttcttg gcaccatttc catcagaccc actacccgaa gcagaggagg
    12481 ttgacgatat gagtataagc cgcaggaagt ttctcggcac caccgcgtcg gcggtgatcg
    12541 tggctggaac gatggccaaa ggacgcgtat tcggcgccaa cgaccgcgtc aacgtgtgcg
    12601 tcatgggcat taatggccga ggcaacagcc acatcaaggg gttcaattca atccgtcagg
    12661 cccgtgtggc cggcctgtgc gaagtcgata ccacgctgtt cgacagccgt gtggaagagt
    12721 atttcaaacg ggcaaacctc tcggtaccca agacctatgc cgacatccgg gaagtgcttg
    12781 atgacaaaga tatcgacgcc gtctcgtgcg cggcgcccaa ccactggcat tcgctggcca
    12841 ccgtctgggc ctgtcaggcc ggcaaagaca tgtacgtcga gaaacccatg acccacaaca
    12901 tctttgaagg cctcaaggtg gtcgaggctg cgaaaaaata caaccgtatc gtccagcacg
    12961 gcacgcaact ccgaagcaat ccggggttcc aggaaggcat ccaactgctc aaggacggct
    13021 tcatcggcga cgtctatatg gcccgctgcg tgtgttacaa atggcgtgga gacatcggca
    13081 aggccgtccc cggccagccg cccagcacac tccggtggga cctctggcag ggcccggcca
    13141 aggaaaag
//
LOCUS       DAOPBQ010000030        12933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4806_length_12933_cov_4.484470, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000030 DAOPBQ010000000
VERSION     DAOPBQ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4806_length_12933_cov_4.484470"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PKW60_02005"
     CDS             <1..554
                     /locus_tag="PKW60_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98235.1"
                     /translation="RRFLPTSASGPWEWGSPERFGTGTLHDVHGPWGFGPWVNNLDEW
                     RAYWSRDDALLRSETGMPAAHSLEMIRKYGGANIWPPITDYWRHTAAWWGQWDRYRET
                     LGGMPEDEALAEYIRLTQEEQAEAYAVAAAACKNRFPRCGGFLVWMGHDAFPCPANNA
                     VIDFDRNPKPAYYALKKVFRAET"
     gene            657..1262
                     /locus_tag="PKW60_02010"
     CDS             657..1262
                     /locus_tag="PKW60_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPJ98236.1"
                     /translation="MKKTPGHNLEPHYRMLTREHLEARTRHPVHIVLENIRSAFNVGS
                     IFRTSDAGAVEHIHLCGYTCHPPNLKLAKTALGAFDYVPWTHHEEPADAVKHLRGMGI
                     PVVAIETADNARSVGTYIWPQPVAIVFGNEVKGISPELLSECDEIVRIPMLGYKNTIN
                     VATAFGIVLYDILGQWGALREPAASAGPKNQKPAPCPDEGQ"
     gene            complement(1207..1647)
                     /locus_tag="PKW60_02015"
     CDS             complement(1207..1647)
                     /locus_tag="PKW60_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HPJ98237.1"
                     /translation="MHKMIAGLCAALLITAGCVSAKSAGPVRPPAASFAAKTMYSDNT
                     GQCLRHVVLFKWKDGTPPETVSEIEQAFQALPGQIPQILALEWGTDMSVENLSQGFSH
                     CFLVSFKNENDRAVYLPHPAHKAFGELIGPHLDKVLVFDFWAQR"
     gene            complement(1659..2615)
                     /locus_tag="PKW60_02020"
     CDS             complement(1659..2615)
                     /locus_tag="PKW60_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98238.1"
                     /translation="MRKSNRLIASVWEGLLLAWGSRACWGYRILVHAPVFLAGVAAIA
                     GAGFIAHLTGFIAALLVAALCAGTLAVLVYNGVVTVSLLASADVEHGLVLAELAQKHE
                     VPDGPGQLAWARQRAAVADAPPVWVLLRIRRAMQAIHERMNDVGDVFPAFAPDRAPSW
                     ARWLLARCTSPMAGALAARAFADTSGNTASRARESLLLYTAVWHDAFRWNAALWGTGW
                     MFMMGAVALAALPLMWAAAASVLAVQATAAMLALWTGFALQRLVMRPILEAAALVMLE
                     EMTQGSSAPPDAEPRLREVSAAFRELENRAQEASELENGQSV"
     gene            2779..3687
                     /locus_tag="PKW60_02025"
     CDS             2779..3687
                     /locus_tag="PKW60_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPJ98239.1"
                     /translation="MAVFGGIEAGGTKFVCALGSRPDDLREEERFPTTTPEETIGRCI
                     ACFEKHARREPITAIGISSFGPVDPNPESATWGYITSTPKPGWGQTPFAGRIAEALGV
                     PVGFDTDVNGAALGENRWGAAQGLDSFIYLTVGTGIGGGGLLGGRLMHGLIHPEMGHV
                     RLPRDKDRDSFEGVCPYHGDCLEGLASGPAMEKRWGTRAEALPDSHPAWDLEAHYLAV
                     ALAGFICTLSPQRIILGGGVMERRTLFPKIRENVLAVLNGYVQSPVILDHIDKYIVPP
                     GLGSKAGVLGAIALAQDAARKAGGHG"
     gene            3703..4725
                     /gene="queA"
                     /locus_tag="PKW60_02030"
     CDS             3703..4725
                     /gene="queA"
                     /locus_tag="PKW60_02030"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPJ98240.1"
                     /translation="MNTSELDYALPEELIAQQPSERRDESRLLVLNRADRSMTVDVFR
                     NLPQYLRRGDCLVFNDTRVIRARLHGRKPTGGQVEVFLLRETAPGEWDALVRPSSKVK
                     PGTPVKIAAGVEALVGDRLPGGRRRVSFAQSDVLAVLERAGEVPLPPYIHRDTPEASD
                     LTRYQTIYASAPGAVAAPTAGLHFTEDVFRRLDETGVARAFLTLHVGYGTFRPIQAEQ
                     LENHALESEPFTLSADTAAQLNGTRAAGGRIIAVGTTVTRVLETQCQGGSFQAGTGET
                     NRYIYPPYTFRAIDALQTNFHLPRSSLLALVCAFGGVDFILDAYRFAVKERFRFYSYG
                     DTMLIL"
     gene            complement(4764..7853)
                     /locus_tag="PKW60_02035"
     CDS             complement(4764..7853)
                     /locus_tag="PKW60_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HPJ98241.1"
                     /translation="MLDKITELSLKMRGLVIIALLAVVAYGIFAYRNMPVDAFPDISP
                     VMVPVFVEAHGMAPEEVERLVTFPIESAMNGLPGVTQIKSTSAFGMAVIYVYFHDRVD
                     MYFARQLVAERLNSSLAELPDLGEPPTLGPISTGLGQIFMYYLTMDETVDTEGKDPDT
                     YLRELNDWQVKAQLRTVPGVTDILSIGGHVLQYQIRLDPAAMLKYDLTLTDIVDAVER
                     NNRNVGGQFLVLDAEETLVRGLGLVQTLDDIRSIPLKSTGGVAVYISDVADVVYGNEI
                     RRGVVTRNGEEEVVSGIVLKLYGENTSQVIKHLYDKVDVVQASLPRGVRLVPYYEQSE
                     LVQQAAWTVTKALLQGAFLVLLTLLLFLGNVRTALIVAVSLPVCSLIAVIFMGLSGIS
                     ANLMSLGGIAIAIGMLGDGSIVMVENIYRHLTGGNEPAGDPRARIASAAREVSRPIVF
                     SIFIIVAVFLPLFTLEGTEGKMFSPMAFTISFALLGSAVTALVVTPVLASLLLAPRVT
                     KEMALLRLLKKAYSPFLNASIRHAGVVLGVALAVFAATLLLVPKLGTEFIPTLEEGSI
                     LIGVTMAPSISLERATETIKMLERQIRKHPEVVETVSRIGRPESGSHPHPVNYAEIHL
                     ALKPLEEWPPDRRKEMLVEELTHELETVPGIQLNFSQPIQNAFDELLSGIKSQLSIEV
                     YGEDLAVLREKAGEIEAVLENVPGLVDLAVEQNFGQPQVQIIPDRQACARLGVPLDHL
                     LEVVELAIGGEVIGQIYQNMRRFGIHVRLQEPYRAEPEAIRRILIPTDTGGRIPLSQV
                     ATVEHVVGPIQVNRENNNRRWVVQANVRGRDLGSVVSDIKAIVASRVTLPEGYWVEYG
                     GQFENQQRAMRRLAIIVPVVLAAVFLLLWMAFGTAREALIILFNVPLALIGGVLGLYF
                     SGEYLSVPASVGFIALFGIAVQNGVVLVSYFDELRKSGHTKIAAVQEGALLRLRPVLM
                     TASTTMLGLLPLLMARGIGSEVQRPLAVVVVFGLGTSTLLTLFVIPAAYAWFGPRLK"
     gene            complement(7889..9220)
                     /locus_tag="PKW60_02040"
     CDS             complement(7889..9220)
                     /locus_tag="PKW60_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPJ98242.1"
                     /translation="MRTKIGVAATVLGALALAAATAYLAYEFARPTQLSLAEAAGAGP
                     ALHVHDHDEHGEEEHGEEASHAEHEHSAPAAPGHDGETGEHGEHFPEVHLPETERQAL
                     GLVLREAAPGKLTVEVELPAEVRLNEDHVAHIVPRVSGIVREVHKHIGDRVLKGELMA
                     VLDSRELADSQAEYLAANSRLELARKEFTREKSLWEAQISAERDYLAASRELQEAQIA
                     VRAAGQKLQALGLSEAQLRDMASGRRDFLTRYEMTSPFDGEVLEKHIVLGEALESQAN
                     AFIIADLDTVWVDVQVFEKDLPHIRPGMTVQISGPEGHSATEGVITYVAPVVDRETRT
                     TLARIVLPNTDRRWRPGLFLRVQLRLEQAEAAVLIPKTAVQRFESAPVVFVEDGDELR
                     AVEVELGHENSDSVEIVSGLPAGTRYVAEGAFELKARIVTSNLGAHAGHGH"
     gene            complement(9210..10727)
                     /locus_tag="PKW60_02045"
     CDS             complement(9210..10727)
                     /locus_tag="PKW60_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPJ98243.1"
                     /translation="MSVCAVLAIGCLTALGAENDSAGAEASSSPNSARRERENGVPLT
                     LQEAIHRALAENPGLAAKRWDVEISGAEVTQAGLRPNPELSIEMEDIRWNRGPTQHTR
                     SRSLAGGLGSGSLPTLGWESETESGSHSGLTEAEVTLSIAQLIELGGKRAKRVAEAEE
                     AKQLALWDYEALRADVIAETARAFVAVVAAQEQLALETDLLRLAEEMVRTFQLRVQAG
                     QESPIEAAKADVALANQRIARERAERALEVVRTQLAAMWGEETPHFGPVEGDLEDLPD
                     LPAFEALRARVSSNPDIARWAAELAARKATLELARANRIPDATVELGLRSAGLADSAA
                     SHYGLGTDGGFSASRSKTSFDSGRDNALQLKFSIPLPIFHRNQGEIAAAQFSVSQIPD
                     SNRAAAMRVHSDLAAAHEEAASAFSEVLALRKNVLPRSAEIFEKTQTGYRQGKFDYLD
                     LLDSQRTLFEVRTAYLTALERGHLAVIALERLTGTALAQYNTASLHDMEETEDAH"
     gene            complement(10829..11260)
                     /locus_tag="PKW60_02050"
     CDS             complement(10829..11260)
                     /locus_tag="PKW60_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98244.1"
                     /translation="MIENKVVKILTWWMTGLCVWAFASGAQEAVLCLGANGHVAIESA
                     HSAPCNIASEAPDDQGSRISGIREHYHSSCVDILFPQQSELAPEDNHKAASKSLETHL
                     AVACESVSEAELAASTAIQPIQSHPLELLSARTLRSTILLI"
     gene            11533..11724
                     /gene="rpsU"
                     /locus_tag="PKW60_02055"
     CDS             11533..11724
                     /gene="rpsU"
                     /locus_tag="PKW60_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPJ98245.1"
                     /translation="MTKVRVKTDEPFEKALRRFKKKCNKEGLMQRLKEMKHYEKPSER
                     RRRKLAKAIARARQDQAGN"
     gene            11840..>12933
                     /gene="xylB"
                     /locus_tag="PKW60_02060"
     CDS             11840..>12933
                     /gene="xylB"
                     /locus_tag="PKW60_02060"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HPJ98246.1"
                     /translation="MSIVLGLDVGTSGTKAVAINASGELVASAQVEYPLLTPEPGWAE
                     QSPAEWKRAAFEALAMLAQSPNMSPADVKGIGLTGQMHGSVFLDQDHRVLRNAILWCD
                     QRTAKQCDYITEKVGERELIEMVCNPAMTGFTAPKILWLRDNEPDVYDRVRKVLLPKD
                     YVRYELTGEFATDVADASGTLLFDVTNRRWHRGLMELLDIPAEFMPRAYEGPEITGVL
                     SKEAARITGLPEGIPVVAGGGDQAAGGVGCGIVRTGVISSTVGTSGVVFAFSDQVSLD
                     PNGRVHTFCHSVPGKWHMMGVVLSAGGSLRWYRDALCAEEKAKAVETGRDPYEYITEA
                     AATVPLGAEGLLFLPYLTGERTPHKDPYARG"
BASE COUNT         2474 a         4183 c         3910 g         2366 t
ORIGIN      
        1 gccgccggtt cctgcccacg tccgcgagcg gaccctggga atggggttcg cccgagcgtt
       61 tcggcacggg cactctgcac gacgtgcacg gtccctgggg gttcggcccg tgggtgaaca
      121 acctcgacga gtggcgcgcg tactggtccc gcgacgacgc cttgctccgc tctgaaaccg
      181 gcatgcccgc cgcgcacagc ctcgagatga tccgcaagta cgggggagcg aatatctggc
      241 cgccgatcac ggactattgg cgtcacaccg ccgcctggtg gggccaatgg gatcggtatc
      301 gggaaacgct tggcggcatg ccggaggacg aggccctggc cgaatacatc cggctgacac
      361 aggaggagca ggccgaggca tacgcggtcg ccgccgcggc gtgcaaaaac cgctttccac
      421 gatgcggcgg ctttctggtc tggatggggc acgacgcctt tccctgcccc gccaacaacg
      481 ccgtcatcga ctttgaccgc aaccccaaac ccgcgtatta cgcgttgaaa aaggtgtttc
      541 gcgccgagac ctgatgggtt gatccccgtc ccggcgcatg acgcctggat agcaagtcac
      601 gcttctgcgt tatgctgccg cagcgggggg aaaaatgtaa aaagcgaatc agtgccatga
      661 agaaaacgcc aggccataac cttgaaccgc attaccgcat gctcacccgg gagcatctcg
      721 aagcgcgtac gcgccatccc gtgcacatcg tgctcgagaa tatccggagc gccttcaacg
      781 tcggctcaat cttccgcacg tccgacgcgg gcgcggtcga acatatccat ctctgcggct
      841 acacctgcca tccgcccaac ctgaagcttg ccaagaccgc tctgggcgcg tttgattacg
      901 tcccgtggac ccatcacgaa gaaccggccg atgccgtgaa acacttgcgc gggatgggca
      961 tccccgtggt cgccattgaa accgctgaca acgccagatc ggtgggcacg tatatctggc
     1021 cgcagcccgt ggccatcgtg ttcggaaacg aggtcaaagg gatctcgccg gaactcctga
     1081 gcgaatgcga cgaaattgtg cgtattccga tgctgggcta taaaaacacc atcaacgtcg
     1141 ctacggcgtt cgggatcgtc ctctacgaca tcctcggcca gtggggtgca ctgcgcgagc
     1201 ctgcagctag cgctgggccc aaaaatcaaa aaccagcacc ttgtccagat gagggccaat
     1261 aagctctcca aaggccttgt gtgcggggtg cggcaggtac acggcgcgat cgttctcgtt
     1321 tttgaacgat acgaggaagc agtgcgaaaa tccctgcgac aggttttcca cgctcatatc
     1381 cgtgccccat tccagcgcga ggatctgcgg aatctggccg ggcagcgcct ggaacgcctg
     1441 ctcaatttcg ctgaccgtct ccggaggcgt gccgtctttc cacttaaata acaccacatg
     1501 gcgcaggcat tgcccggtgt tgtccgaata catcgttttt gccgcgaagg atgccgctgg
     1561 cgggcgcaca gggcctgccg atttggctga cacgcatccc gccgtaatca aaagcgccgc
     1621 gcatagccct gcgatcatct tgtgcatacc cgctcctttc agacagactg cccgttttcc
     1681 aactcagagg cctcctgggc ccggttttcc agttcacgaa acgccgcgct gacctcgcgc
     1741 aaccgcggct cggcatccgg cggcgccgag ctgccctggg tcatctcctc aagcatcacc
     1801 agggccgccg cttcgagtat cggcctcata acgaggcgtt gtagcgcaaa gcccgtccac
     1861 agcgcaagca tggccgcggt ggcctgcact gccaggacac tggccgccgc cgcccacatc
     1921 agcggcaggg ccgccagcgc aactgcgccc atcataaaca tccatcccgt gccccacaac
     1981 gcggcgttcc accggaacgc gtcgtgccag actgccgtgt agagcagaag ggactccctg
     2041 gcccgcgagg ctgtgtttcc ggacgtgtcc gcaaacgccc gggctgccag ggcgcctgcc
     2101 atcgggctcg tgcagcgggc gagaagccag cgtgcccacg acggcgcacg atccggcgca
     2161 aacgccggga acacgtcgcc cacgtcgttc atcctttcgt gaatggcctg cattgcccgg
     2221 cgaatgcgca aaagcaccca cacgggcggc gcatcggcca cggcggcgcg ttgccgcgcc
     2281 cacgccagct ggccgggccc atccggcact tcgtgctttt gcgccagttc cgccagcacc
     2341 agcccgtgtt ccacgtcggc actggcgagc agcgacaccg taaccacgcc gttatagacc
     2401 agcacagcca gcgtccccgc gcagagtgcg gccaccagca atgcggcgat aaaccccgtc
     2461 aggtgggcaa taaatccggc cccggcaatt gccgccactc ccgccagaaa cacaggggca
     2521 tgcaccagga ttcggtagcc ccagcaggcg cgcgaacccc aggccagcaa caggccttcc
     2581 catacactcg caattaaccg gttagacttc cgcatggcac gctattatct acgccgcccg
     2641 tcctgcattc caaacgggag ctgcggacgt tccgccggcc ctccgagccg ccgagacatt
     2701 gacagcacct ccgacgtccc catatagtcc cagtggacct gaacactttc cctgaacacc
     2761 tcgaacggag aagcagcgat ggcggtattc ggaggcatcg aggcgggcgg cacgaagttt
     2821 gtctgcgccc tgggctcgag accagacgac ctgcgcgagg aggagcggtt tcccaccacg
     2881 acgccggagg aaaccatcgg ccggtgcatc gcctgcttcg agaaacacgc ccggcgggaa
     2941 cccatcaccg ccatcggcat cagttccttc ggtcctgtgg acccgaaccc ggaatccgcc
     3001 acctggggct acatcaccag cacgccgaaa cccggctggg gccagactcc ttttgcgggg
     3061 cgcatcgccg aagccctcgg cgtgcccgtg ggattcgata cggacgtcaa cggcgccgcg
     3121 cttggcgaaa accgctgggg cgctgcccag gggctcgatt ccttcattta cctcaccgtc
     3181 ggcacgggca tcggcggcgg cggcctgctc ggtgggaggc tcatgcacgg gctgattcac
     3241 cccgagatgg gccacgtccg cctgcctcgc gataaagacc gcgactcctt cgagggcgtg
     3301 tgcccgtatc acggcgactg tctcgaaggg ctggcctcgg gaccggctat ggagaagcgc
     3361 tggggaaccc gcgcggaggc tctgccggac tcgcaccccg cctgggatct cgaagcacac
     3421 tatctggccg tcgcgctggc aggattcatc tgcactttgt caccgcaacg catcatcctg
     3481 ggcggtggcg tgatggagcg ccgcaccctc ttcccgaaaa tccgtgaaaa tgtcctcgcg
     3541 gtgctcaatg gatacgtgca atctcctgta atcctcgatc acatcgacaa atacatcgtt
     3601 cccccggggc tgggcagcaa agcgggcgtg ctcggcgcca ttgccctggc ccaggatgcc
     3661 gcccgaaagg ccggcgggca cggataaatc cggacagctg ccatgaacac ctcagaactc
     3721 gattatgcgc ttccggaaga actcatcgcg caacagccca gcgaacggcg cgatgaatcc
     3781 cgcctgcttg tgctcaaccg cgcggatcgc tccatgaccg tcgacgtctt ccgcaacctg
     3841 cctcaatacc tgcggcgtgg cgattgcctg gtgtttaacg acacccgcgt catccgggcg
     3901 cgcctccacg gccgaaagcc taccgggggt caggtcgaag tgtttctgct gcgcgagacc
     3961 gctcccggcg agtgggacgc cctcgtgcgg ccgtcatcca aggtcaagcc cggcacgcct
     4021 gtgaaaatcg ccgccggcgt cgaggccctg gtcggtgatc ggctccccgg cgggcgccgg
     4081 cgcgtctcgt tcgcccaatc ggatgtcctg gccgtgctgg agcgggccgg cgaagtccct
     4141 cttccgccat acatccaccg cgatacgccc gaggcatccg atctgacgcg ctaccagacc
     4201 atctacgcca gcgcgcccgg cgccgttgcc gcgcccacgg ccggcctgca tttcaccgaa
     4261 gacgtgtttc gccgtctcga cgaaactggc gtggcccgcg cattcctgac cttgcacgtg
     4321 gggtacggca cgttccggcc catccaggcc gagcagctgg agaaccatgc cctggagtcc
     4381 gagccgttta ccctcagcgc agacaccgcc gcacagctca atggcacccg cgccgcgggc
     4441 gggcgcatta ttgccgtcgg cacgaccgtg acccgggtgc tcgagaccca atgccagggc
     4501 ggctcctttc aggccggcac gggcgaaacc aaccgctaca tttacccccc ctataccttt
     4561 cgcgcgatcg atgcgctgca gaccaatttt cacctgcccc gctcaagcct tctggccctg
     4621 gtctgcgcgt ttggaggggt tgatttcatc ctcgacgcat accgtttcgc tgtcaaggag
     4681 cggtttcggt tctattccta tggcgacacc atgctgatcc tctgactccg agattgcccg
     4741 tgcccgcagc tcgcccgcgt gtcttatttc agtcgcggac cgaaccaggc gtaggctgcc
     4801 gggatcacga agagagtcaa gagcgtggac gtgcccagac cgaacacgac cacgacggcc
     4861 agcgggcgct gcacctcgga gccgatgccg cgggccatca gcagcgggag cagcccgagc
     4921 atggtggtgc tggcggtcat gagcaccggc cgcagacgaa ggagtgcgcc ttcctgaacc
     4981 gctgcgatct ttgtgtgccc gctcttgcgc agttcatcga agtagctgac gagaacgacc
     5041 ccgttctgga ccgctatccc gaacagcgca ataaacccta ccgacgccgg gacggacagg
     5101 tattcgccgg agaagtacag gcccagaacg ccgccgatca gcgccagagg cacgttgaaa
     5161 aggatgatca gcgcttcccg ggcggtgcca aacgccatcc agagcagcag aaacacggcc
     5221 gcaagcacaa ccggcacaat aatggccagc cgccgcatcg cgcgctgctg attttcgaac
     5281 tggcctccgt actccaccca atagccctcc ggcagggtga cgcgggacgc cacgatggcc
     5341 ttgatgtcgc tgacgacact gcccaaatca cggccgcgca cgttggcctg gaccacccag
     5401 cggcggttgt tgttttcgcg attgacctgg atggggccga cgacgtgctc cacggtggcg
     5461 acctggctca gcgggatcct tcccccggtg tcggtgggga tcaaaatgcg ccggatcgct
     5521 tcaggctccg cgcggtacgg ttcctggagg cgcacatgga tgccgaaacg gcgcatattc
     5581 tggtagatct ggccgatcac ttcgcctccg atggccagct caaccacttc gaggaggtgg
     5641 tcgagcggga cgcccagccg cgcgcaggcc tgccggtccg ggatgatctg cacctggggc
     5701 tggccgaaat tctgttcaac agccaaatcg accaagccag ggacattttc gagcacggcc
     5761 tctatttcac cggccttctc acgcagcacc gccagatctt cgccgtatac ctcgatcgac
     5821 aattgggatt tgatgcccga cagcagttcg tcgaaggcat tctgaatcgg ctggctgaaa
     5881 ttgagctgaa tccccgggac cgtctccaat tcgtgggtca gctcttctac gagcatctct
     5941 ttccggcggt ccggcggcca ttcctccagt ggtttcagtg cgaggtggat ttctgcgtaa
     6001 ttcaccgggt gcggatggct tcctgactca ggccgcccga tgcgcgacac cgtctcgacg
     6061 acttcggggt gctttcggat ttgccgttcc agcattttga tagtttcggt ggctcgttca
     6121 agagaaatgg acggcgccat ggtcacgcca atgagtatcg acccttcttc gagcgtgggg
     6181 atgaactccg tgcccaattt cggcacgagc agaagtgtgg ccgcgaacac ggcaagtgcc
     6241 acgcccaaca ccaccccggc gtgacgaatc gaggcattca agaaagggga gtaggccttt
     6301 ttgagcagcc ggagcaatgc catctctttc gttacgcgcg gagcaagcag cagcgatgcc
     6361 agcacgggcg tcaccacgag ggccgttaca gcggagccca gcagcgcaaa cgaaatcgtg
     6421 aacgccatgg ggctgaacat cttcccttcg gtgccctcca gtgtgaagag aggaagaaac
     6481 accgcgacaa tgatgaagat cgaaaataca atgggccggc tgacttcgcg cgctgccgat
     6541 gcgatccggg cccggggatc tcccgccggc tcgtttccgc cggtcagatg acggtagata
     6601 ttctcgacca ttacaatcga gccgtcgccc agcatgccga tcgcgatcgc aatgcccccc
     6661 agcgacatca ggttggccga aatgccgctc aggcccatga aaatgaccgc gataagcgag
     6721 cacaccggca gcgaaaccgc cacgatgagc gccgtgcgga catttcccaa aaacaaaagc
     6781 agggtgagaa gcacaaggaa tgcgccttgc aacagcgcct ttgtgaccgt ccatgcggcc
     6841 tgttgaacga gttccgactg ctcgtaatac ggtaccagac ggacacccct gggaagagac
     6901 gcctgcacga catccacttt gtcgtacaag tgtttgatca cctgcgaggt gttttctccg
     6961 taaagcttca agacaatccc ggacacaacc tcctcttcgc cgttccgcgt gaccaccccg
     7021 cgccgaatct cgttgccata caccacgtcc gccacatcgc tgatgtagac ggcgactcct
     7081 cccgtgcttt tcaacggaat ggagcggatg tcgtcgaggg tctgcaccag ccccagcccg
     7141 cgcacgagcg tctcttcagc atccagcacg agaaactgcc cgccgacgtt gcggttgttc
     7201 cgctccaccg cgtcaacgat gtccgtgagt gtcagatcgt atttcagcat tgccgcgggg
     7261 tcgagacgaa tctgatattg caggacatgg ccgccaatcg agaggatgtc ggtcaccccg
     7321 ggaaccgtcc gcaactgcgc cttcacctgc cagtcgttca attcgcgcag gtaggtgtcg
     7381 gggtctttcc cttcggtgtc cacggtttcg tccatggtca ggtaatacat gaagatctgg
     7441 ccgaggccgg ttgagatcgg cccgagtgtg ggcggctcgc ccaggtccgg aagttcggcc
     7501 agggaactgt tcaatcgttc cgccaccagc tgccgggcga aatacatgtc cacgcgatcg
     7561 tgaaaataga cgtagatcac ggccatccca aaggcggagg tggatttaat ctgtgtgacg
     7621 cccggcagcc cattcatggc ggattcaatg ggaaacgtga cgagacgttc cacttcttcg
     7681 ggcgccatgc cgtgtgcttc cacgaatacg ggcaccatta cgggggaaat gtcgggaaac
     7741 gcgtcgacgg gcatgttacg gtaggcgaag atgccgtagg caaccaccgc gagcagggcg
     7801 atgatgacca ggccgcgcat cttgagcgag agttctgtga tcttatcgag caccgctgaa
     7861 ttccctttcc ggcaggagcg tcctgcgatc agtggccatg tccggcgtgt gcgcccaggt
     7921 tgctggtgac aatgcgggct ttcagttcaa acgcaccttc cgcgacgtag cgcgtgccgg
     7981 ccggaagccc cgacacgatc tccacgctgt cgctgttctc atggcccagt tccacctcca
     8041 ccgctcgcaa ttcatcccca tcttcgacga acacgaccgg ggcgctctca aaacgctgta
     8101 ccgcggtctt tggaatcaaa acggctgcct ctgcttgctc caggcgcaac tgtacgcgca
     8161 gaaacagccc cggacgccaa cgccggtccg tgttgggaag gacgatgcgg gccagcgtgg
     8221 tgcgggtttc ccgatcaaca accggcgcta cgtaggtgat cacgccttcc gtcgcggaat
     8281 ggccttccgg gcctgagatc tgcacggtca tccccggccg gatatgcggc aaatccttct
     8341 cgaaaacctg cacgtccacc cacaccgtat caaggtcggc gatgataaag gcgttggcct
     8401 gcgactccag cgcctccccc agcacgatgt gtttctcgag cacttcaccg tcgaagggag
     8461 aggtcatctc gtaccgggtc agaaaatcac gccggcctga agccatatcg cgaagctgcg
     8521 cttcagacaa gcccagcgcc tgcagtttct gccccgcggc acgcacggcg atctgcgctt
     8581 cctgcagttc ccgggaagcg gcgagatagt ctcgttccgc cgagatctgg gcctcccaca
     8641 gggatttctc ccgagtgaac tctttgcgcg caagctcgag gcgcgaattt gctgccaggt
     8701 actccgcctg gctgtccgcc agctcgcggc tgtccagtac ggccatgagc tcgcctttca
     8761 gtacccggtc tccgatgtgc ttgtgcacct cgcgcacgat gccggaaacg cgcggcacga
     8821 tgtgcgcgac atggtcttcg ttcagccgga cttcggcggg tagctccact tccacggtca
     8881 gcttcccggg agccgcttcg cgaagtacaa ggccaagggc ttgccgctcc gtttccggca
     8941 gatggacttc ggggaaatgt tcgccgtgct ccccggtttc gccatcgtga ccaggcgctg
     9001 cgggtgcact gtgctcatgt tccgcgtgac tggcctcttc gccgtgctcc tcttcgccgt
     9061 gttcgtcgtg atcatgcaca tgcagtgcgg gcccggctcc ggccgcttcg gcaagagaca
     9121 gctgagtggg ccgcgcgaat tcgtaggcca ggtaggctgt cgcggccgcc agggccagtg
     9181 cgccgagaac cgtggctgca acgccgattt tagtgcgcat cctctgtttc ctccatgtcg
     9241 tgaagcgatg ccgtattgta ttgagcgagt gctgtgccgg tgagccgttc cagcgcgatt
     9301 accgccaggt ggccgcgttc cagtgcggtc agatacgccg tccgcacctc aaacagcgtg
     9361 cgctgggagt cgagcagatc caggtagtcg aacttgccct ggcgataccc cgtttgggtc
     9421 ttttcgaaaa tttcggcgga ccggggcaga acatttttgc gaagcgccaa cacttcggaa
     9481 aacgccgacg ccgcctcttc gtgagcggca gccaggtcgc tatggacgcg cattgcggcc
     9541 gcccggtttg aatccgggat ctgcgagacg ctgaactgtg ccgccgcgat ttcgccctga
     9601 ttccggtgaa agatgggcag ggggatggaa aacttcaatt ggagtgcatt gtcgcgccct
     9661 gaatcgaagc tcgtcttcga gcgtgatgca ctgaaccctc cgtccgttcc gagcccgtag
     9721 tgcgaggcgg cgctgtccgc caggcccgcg ctgcgaagcc ccaattccac cgtcgcgtca
     9781 gggatccggt tggctcgcgc cagttcaagc gtggcttttc gtgcggccag ctctgctgcc
     9841 caacgggcga tgtcgggatt gctgcttaca cgcgcgcgca gggcttcaaa ggcgggcaga
     9901 tcgggcaggt cttccaagtc accctcaacc gggccaaagt gaggtgtctc ctcgccccac
     9961 atcgcggcaa gctgggtgcg aacaacctcc agcgcccgct cggcacgttc ccgcgcgatg
    10021 cgctggtttg cgagggccac gtccgctttg gccgcttcaa tgggggattc ctggcctgcc
    10081 tgaacccgca gctggaatgt ccgcaccatt tcttccgcca accgcaacag gtcggtttcg
    10141 agagcgagct gctcctgggc ggccaccacg gccacaaatg cgcgcgctgt ctccgcgatc
    10201 acgtcggcac gcagggcttc gtaatcccac agggccagtt gcttcgcctc ttcggcctcg
    10261 gcgacccgct tcgcccgttt gccgcccagc tcgatgagtt gagcgatgct cagcgtgacc
    10321 tcggcttccg tgagccctga atgcgaccct gattccgttt cagattccca gcccaaggtg
    10381 ggcaggctcc cggaaccgag cccgcccgca agcgaccggc tgcgcgtatg ctgggtcggc
    10441 ccgcgattcc accggatgtc ctccatctca atcgatagtt cgggattggg gcggagaccc
    10501 gcctgggtta cctcggcccc gctgatctcg acatcccacc gtttggcggc cagaccagga
    10561 ttctcagcga gggctcgatg aatggcctcc tgcagcgtga gcgggacgcc gttttcccgt
    10621 tcacgccgcg cgctgttggg cgatgaagag gcttcagcgc ctgccgaatc gttttccgca
    10681 cccaatgccg tcagacatcc gatggcaagt actgcacata cactcatgaa ccgcataacc
    10741 gctcctttcc aaggggatgg cgcacaacag ggcgttttcg cgcagagaaa gggcccgcca
    10801 gggcccgcgc acaaagggag gtatgcggtc agatcaaaag aatggtgctg cggagggttc
    10861 gcgcggaaag gagttccagg ggatgggatt gaattggctg tatggccgtg gaggcggcca
    10921 gctcggcctc agaaacgctc tcgcaggcga ccgccagatg cgtctcaaga gacttcgacg
    10981 ccgccttgtg gttgtcttcc ggggccaatt cggattgctg cggaaacagg atgtccacac
    11041 agctgctgtg ataatgctca cggatgccgc tgatgcggct gccctggtca tccggtgcct
    11101 cagaggcgat gttgcacggg gcgctgtgcg cgctttcgat ggccacgtgg ccattcgcgc
    11161 ccaggcacag caccgcttct tgcgcgccgg aggcaaacgc ccacacgcac aagcccgtca
    11221 tccaccacgt cagaattttt accactttat tttcaatcat acggcaggga tacgcctttt
    11281 aatgcaaagt ataggcgatc ctcactgcaa aaacacgttt ttgcgggtgg ctattccaac
    11341 gcaggccgcg cggggtactg ccggacgacg ttgtgtttga cagcaagggc gttgaatgat
    11401 agaatacccc tccggttgcc aaggctgcgt aacccctttg gaagatgggc tttcgaggcg
    11461 ttagagcgat catcggcaac gaaaaaaggc gcgcatgaaa aaggcgcgcg acagagggga
    11521 ggttgattcc gagtgacgaa agtgcgcgtc aaaacagacg agccctttga aaaggcactc
    11581 cgccggttca agaaaaaatg caataaagaa gggctgatgc agcggctgaa agaaatgaag
    11641 cattacgaaa agcccagcga gcgccgccgc cgtaagctcg ctaaagctat tgcgcgtgcc
    11701 cggcaggatc aagcgggtaa ttgagcttcc tcgccaatga gtgttatttt gaggcaggat
    11761 cgagtcatcg atcctgcctc gttgttttag agcgtactcc ggcgccggga gcgcggccgc
    11821 tggaaagggg gatgtaccga tgagcattgt gctggggctg gacgtcggca cgagcggcac
    11881 caaggcggtc gcgatcaacg cgtcgggcga actggttgca tcagcccagg tcgaataccc
    11941 gctcctgacg ccagaaccag ggtgggcgga gcagtcccca gccgaatgga aacgcgcggc
    12001 cttcgaagcc ctcgccatgc tcgctcagtc acctaatatg agtcccgccg acgtgaaggg
    12061 catcggcctg accggccaga tgcacggctc ggtctttctc gaccaggacc accgggtgct
    12121 gcgcaacgcc atcttgtggt gtgaccagcg caccgcgaaa cagtgcgact atatcaccga
    12181 aaaggtgggc gagcgggagc tgatcgagat ggtctgcaac cccgccatga caggtttcac
    12241 cgcgccgaaa attctgtggt tgcgcgataa cgagccggac gtctatgacc gggtgcgcaa
    12301 ggtgctcctg ccgaaggatt atgtgcgcta cgagctgacc ggcgaatttg ccaccgacgt
    12361 ggccgacgca tcgggcacgc tgctttttga cgtgacaaac cggaggtggc atcgtggatt
    12421 gatggagctg ctcgatattc ccgcggagtt catgccgcgt gcgtatgaag gccctgagat
    12481 cacgggcgtg ctttcgaaag aggccgcgcg cattaccggc ctgcccgaag gcattcccgt
    12541 ggtggccggc ggcggcgatc aggctgcggg cggtgtggga tgcggcatcg tgcgcacggg
    12601 cgtcatctca tcgaccgtgg gcaccagcgg cgtggtcttc gccttttccg accaggtcag
    12661 cctggatccc aatggccggg tgcacacctt ctgccactcc gtgccgggaa aatggcatat
    12721 gatgggcgtg gtgctgagtg ccggcggctc gctgcgctgg tatcgcgatg ccctgtgtgc
    12781 cgaagaaaag gcaaaagccg tggagacggg ccgggatccg tacgaataca tcacggaggc
    12841 cgcggcaaca gtgccgctgg gcgccgaggg cctgctcttt ctcccatacc tgaccggaga
    12901 acgtacgccg cacaaagacc cgtacgccag ggg
//
LOCUS       DAOPBQ010000031        12932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4807_length_12932_cov_3.870777, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000031 DAOPBQ010000000
VERSION     DAOPBQ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12932
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4807_length_12932_cov_3.870777"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2738
                     /locus_tag="PKW60_02065"
     CDS             <1..2738
                     /locus_tag="PKW60_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPJ98247.1"
                     /translation="ITVMVTVYVVLAGIIAIVFCVGVIAILCLNAYLNIHNSIVRPLS
                     LLNEGAQIIGQGDLDLKLKIGTGDEIEELAQSFNKMALALKRNIGQLEDSEEKYRGLV
                     TWMRDGVFQTNPQWIVGFMNPAGVEIFRHEDVEEIIGRNISDMFYNPSDFTQLARELQ
                     HKGFIERRRLWMRRRDERAIIVELSANTIYGDDNSVIGYEGIFRDLSKSVRLEREARD
                     RAERLSAISQIANVINSSLEAGRLYESLVVEIKRLIDFDFASLALLDELGETFEIRQL
                     WPEAKAIELETGAMHDETSCAAWVARERERLMVGDLTRDDVRFSNAEFAPGTRSCLCV
                     PLYATGRIIGTFNLGSERPDAFRRHDAAVLEQVAPHVAVAIRNANLLENLQVSLEDVT
                     LAREKLHEANEELKSLDEMKTNLLSNVSHELRTPLVAVMGYTDMIYNGKAGPVTETQK
                     DYLGISLRNIEKLVTLIENLLDFSRLHRGTETIVFGVFDLVECAKASIQTMKPVADSQ
                     GVELVLQAPDEPQLVEGDKSKLGQVFNNLLSNGVKFNHKGGNVTVALRPGTEDIEVTV
                     SDTGIGIPPDALDKVFTRFYQLDGSSTRKYGGTGIGLSIAQDIARLHGSRITVTSEVG
                     EGATFRFTLPFTKLVRGQSLVQAAVDEPAGPLVTEILVELLTRDSDLSNQVRALLSSE
                     SVNSIQAQDAAQAIALARRHSPDCILVDADASEENERLLDVLLADATIQTLPMVLVTN
                     DEDLFEKYRSFISARLKREFRKSTLLSSIHNAVRQPIVPESSLGGKILCVDDDPDVLV
                     FLRRCLETDGYEIDECESGEEALARLRSREYALVLLDIAMPGMDGWETCLRIKSDPAL
                     AGIKVFMVTAKPLDAKNPLTKSSGCDGFIQKPFRAEDIVEIASRLLKRPHAIRRA"
     gene            2742..4517
                     /locus_tag="PKW60_02070"
     CDS             2742..4517
                     /locus_tag="PKW60_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98248.1"
                     /translation="MEDPLRLAGLASNRPFRAFVHFNRPYARAYVIGAAAGLFFITID
                     LCMPMVVRAVVHRLENRTLDYRFLLTCVAFLLAIPICSGLARFVQRMLIIKASREFEY
                     DLRNVFYRKIQHLSQEFFHSLRTGDIMARATNDLNYVRMFVGPGVMNTVNMIRLPFSL
                     AAMMYLSPKLTGIALIPLPFISFVVYFFMMYMHRQSKRVQEQFSTVTARAQENLAGAR
                     VVQAYGIADREIRDFRNESRKYMRESLKLTIIMALAWPLIGTTIGVSALIILWTGGHM
                     VISQTLSLADLSAFTVYLVMLTWPLVEFGWVLTLYQRGAVSMSRILEILTRKPAVSDS
                     AQTDRSIRSVDGAVGFHGVSFAYNGQNVLKNISVDIPAGQTVAIVGPTGSGKSTLLSL
                     LAREYDPQLGAVLIDGRDVREFPLETLRKHIGCVPQDPFLFSDSIRANLTLGCPEASH
                     EDLHWACEIAQFSDTLQELPNGYDTLLGERGINLSGGQKQRLTIARALICKPRILILD
                     DALSSVDTHTEEEILRRLKRVMRERTSIIVSHRISTVSHADRILVLDDGKIVEEGTHE
                     ELVARGGLYAEMHRRQLLEKEIEQA"
     gene            4514..6403
                     /locus_tag="PKW60_02075"
     CDS             4514..6403
                     /locus_tag="PKW60_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98249.1"
                     /translation="MSEGYHIEDEVQQRAYDRQLMRRLLGYLRAYRSAMILAVVLLLG
                     ASILTNLVPYFNKLALDKYIGNPERIAQQEQAAGGTPSATLEALVTHDRQGLGRVILI
                     IACLMLGETLARYLQNIIVAYVGQKTMLAMRLQIFEHLQRMSLRFLDRNPIGRLMTRV
                     TNDVEKIQETIVAGMVQVISDLLTVFVILAFMLWVNWQLALILMTTVPFVFVTGFVFR
                     RYARRSYIEIRKRLARMNAYAQETISGMRIVQMFTAEEPTYEEYRRRNEEHRDEWFQQ
                     VLNYAVYFPVVDFLGMLAIGLIVLYTGDRFLRFGDAVGVSVGTLFAYIQWSERFYAPI
                     RTIADRYNLLLEAMASSERIFELLGAEEEIENAPDAVIPDRFEGRVEFKDVWFAYEPD
                     HWILKGINLAIAPGERIAIVGHTGAGKTTLINLLSRFYDVQRGSIAIDGLDVRLYEKT
                     ALRQRIGTVLQDVFLFSGSIEQNIRLGNQELTMERVRQCAAYVNAARFIEKMPGGYAY
                     DVGERGNNLSTGQRQLLAFARTLAHDPQLLVLDEATSSVDTETEVLIQDAIRKLMLGG
                     TSIVIAHRLSTVQNADRIIVMHHGEVRETGTHRELLAQRGLYYRLYQLQYKHQNGTSE
                     TTQIP"
     gene            6946..8880
                     /locus_tag="PKW60_02080"
     CDS             6946..8880
                     /locus_tag="PKW60_02080"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPJ98250.1"
                     /translation="MLEFKKGVDILSAIGGIDCIEKARALFKEQLDAEHLTRLSIITN
                     EEVLLKVANAIAMCQPDRVYVLTGSEADKEFVKKFSIEKGEETPLAIPGHTYHFDLPE
                     DQGRLLNQTLYIVNEDEQISSLAQREPRNESHAHIEKYMSGIMKGKTMFVGFYSRGPI
                     GAQASIPAIEISSSTYVLHSAALLYRNCYAEFDAEVERRGEFFTNVHSEGPNRPEDIP
                     NCRIYMDRSWQTTYSMFCTYAGNTLLMKKGNHRFASDSAIYRNYGRELSEHMFITGMT
                     GPGGRKTYFAGAAPSGCGKTTTAMVGSDFVGDDLAQLWIGEDGSLRAINPEIGIFGIL
                     EDVNYAGDPHLMKCLREPGAEVIFSNVLIDADKKPRWVGDGEPVPERGFNFQGEWTPN
                     MTDVPMSHPNSRCTLLSEAIANHNKEANADPSGVQIAVVTYSGRDSDTMPPIWVAKTM
                     ADGVAIGASIVSKATATEIGASGIRRQPWANAPFIAGKLGDYLDAQFKVFTNPDLKQK
                     PVIAGLNYFLTCENRGGGGAGLLGEKRDVHVWLGWLELYAHGDVQALDTPIGMLPKYE
                     DLKALFKEKLDKEYSEELYTKQFSIYVDNVLMLIDLEIAAWQQEESVGQTIFDIYAKQ
                     KAGLEALKAAKGPVVRPQDF"
     gene            complement(9137..11431)
                     /locus_tag="PKW60_02085"
     CDS             complement(9137..11431)
                     /locus_tag="PKW60_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HPJ98251.1"
                     /translation="MYHACPHCHKLLYIPDKYGGLQGRCLQCGGIFTPPRDKWPPFLT
                     KLMDRLTRGDFGEYRIDPYIARARHIDAERQRIQDLCGRLQAEQANLESQLDQLWVAH
                     KTAEFQRHLAARPLDELREIPGIGPVTLRKLEEAGAHNLLDLHARRHIVHNLQGVSQR
                     GKVGIARYVQEAAASARSQPVKLAVDKQEVRRLIELIIDHDWLTGVSMSANRAVMQIA
                     ALARQFPTISFIQYMFGRRGTSAQQTDAAMDALDRIVSFVRDTDRGEKANYSTLSQND
                     YIRAIELLQRRYPQAGEDGTSAPWEGGIPANLTKKIEAFTLDTSGLQNCLLRRYQDFG
                     AKFILVRKQCFLGDEMGLGKTIQTLAAIAHLEACNGKIRALVIVPASLRENWLREVDD
                     KTRLNAFVIRPSQTEQDVDAWQAKGGIGIISYDTLRGKHKALMPRMRFLDFVVADEAQ
                     YIKNPHTKRAKATRAVFAQARYALAMTGTPIENHPEEFLAITRGLRHGNATVSRMTAS
                     RNPGSPDYFKRAVKDLYLRRNKEDVLNELPDLNEINESIVLDRADLKSHLAMMNAGAH
                     FMHLRQHAIFGDGDESPKIARVQELIQEYVENGRSVVVFTYFKRVIDTLSVSFPDAGV
                     IMGAVSTSERMNVIDELNENSPTGKIILAQIGAGGVGLNMQAASAVILVEPQLNPAAE
                     YQAICRVHRMGQRRSVNVHRLTSLHTIEEGIVESLAQKRRYMDDYARDSLLKESSPAA
                     ISAQEMTEVIAAQRQKLSARLAAS"
     gene            11691..12686
                     /locus_tag="PKW60_02090"
     CDS             11691..12686
                     /locus_tag="PKW60_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HPJ98252.1"
                     /translation="MSDKGLFLQNVIAVIWDFDRTLSPQYMQKPLFEAYDVDENTFWE
                     QVGQLPAYYQRSGIHVQRDTCYLGHILSYVRAGRFQGLTNAKLRDLGKNIPFYRGLPF
                     FLDRLKEIVKEPPYVEGDLRLEHYVVSTGLAEIIRGSEIAEKLDGVWASEFIEAPAEP
                     DTDLAGNPRSGEISQIATLLDNTTKTRALFEINKGVNKEPSITVNDAIPEEERRVPFK
                     NMIYVADGPSDIPSFSVVRKHGGLAFAVYKPRSQRHYEQALMLQETDRVDMIGPADYR
                     EGASTDMWFQSQIRRIATRMLDERRRALESKVRKEPVHLAEIQRQKKLKEKEAGE"
     gene            complement(12691..>12932)
                     /locus_tag="PKW60_02095"
     CDS             complement(12691..>12932)
                     /locus_tag="PKW60_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPJ98253.1"
                     /translation="VKAVDTTGCGDSFSAGFLAGMLRWDEPATALACGSVVAGVNARH
                     RGIGQLAEAKRLLSDPRRHFAVFEGKPPDWPGDCI"
BASE COUNT         2825 a         3840 c         3557 g         2710 t
ORIGIN      
        1 acatcaccgt gatggtcacg gtctatgtcg tcctggccgg gatcatcgcc atcgtcttct
       61 gtgtgggggt catcgcgatt ctttgtttga acgcctacct caatattcac aacagcatcg
      121 tgcgcccgtt atcccttctg aacgagggcg cccaaatcat cggccagggc gacctggacc
      181 tcaaactgaa gatcggcacg ggcgacgaaa tcgaggaact ggcgcagtcg ttcaacaaaa
      241 tggcccttgc cctcaaacgg aacataggac aactggaaga ttcagaagaa aagtaccgtg
      301 gactggtcac ctggatgcgc gatggcgtgt tccagaccaa tccccagtgg atcgtcggct
      361 tcatgaatcc cgccggtgtg gagatattcc gccacgaaga cgtagaagag atcattggcc
      421 gcaacatcag cgatatgttc tacaatccca gcgacttcac ccagctcgcc cgggaactcc
      481 agcacaaggg atttattgaa cgccgccgac tgtggatgcg gcggcgggat gaacgcgcca
      541 ttatcgtcga actctccgcg aataccatct acggcgatga caactcggtt attggctacg
      601 agggcatctt ccgcgaccta tccaagagcg tgcgcctgga gcgcgaagcg cgcgaccggg
      661 cggaacgcct cagcgcaatc agccagattg ccaatgtcat caactccagc ctggaagcag
      721 gccgcctgta cgaaagcctg gtcgttgaga ttaaacggct tattgatttt gattttgcca
      781 gcctggctct gctggatgaa ctgggcgaaa cctttgaaat ccggcagctc tggcccgaag
      841 ctaaagccat cgagctggaa accggcgcca tgcacgacga aacctcctgc gcggcgtggg
      901 ttgcccggga acgggaacgt ctcatggtgg gcgatctcac ccgcgatgat gtgcgcttca
      961 gcaatgctga attcgccccg ggaacgcgaa gctgcctgtg cgttccgctg tatgccaccg
     1021 ggcgcatcat tggaacattc aatctgggct ccgagcgccc agacgccttt cgccggcacg
     1081 acgccgctgt gctcgaacag gtggccccgc acgtggccgt tgccattcga aacgccaatc
     1141 tcctggaaaa cctgcaggtc tcgctggaag atgtcaccct cgcccgtgaa aaactgcacg
     1201 aagcaaacga agaactcaaa tccctcgacg aaatgaagac caatctgctg tcgaatgtct
     1261 cccatgagct gcgcacgccc ctggtggcgg tcatgggcta caccgatatg atttacaacg
     1321 gcaaggccgg gccggtcacc gaaacccaaa aggactacct cggcatcagt ctgcgcaaca
     1381 ttgaaaagct ggttaccctg atcgaaaacc tgctcgattt ctcccggctg catcgcggga
     1441 ctgagaccat cgtcttcggc gtgtttgatc tggtcgaatg cgccaaggcg agcattcaaa
     1501 ccatgaaacc cgtcgcagat tcccagggcg tcgagctggt gctgcaggcg ccggacgaac
     1561 cccagctggt cgaaggcgac aaaagcaagc tcggccaggt tttcaacaac ctcctgtcca
     1621 atggcgtcaa attcaaccac aaaggcggca acgtaaccgt ggctctccgg ccaggaaccg
     1681 aggatatcga agtcacggtc agcgacaccg gcatcggcat ccccccggat gcgctcgaca
     1741 aagtgttcac acgcttttac cagctcgacg gctcgtctac ccgcaaatac ggcggcacgg
     1801 gcatcgggct gtccatcgca caggatatcg cccggcttca tggcagccgc atcacggtca
     1861 ccagcgaagt gggggaaggg gccacgtttc gtttcactct gccgttcacg aagctcgtgc
     1921 ggggacagtc gctggtgcag gcagcagtcg atgagcccgc aggccctctc gtcactgaga
     1981 tcctggttga gctattgacc cgggacagcg atttgagcaa ccaggtccgg gcgctgctct
     2041 cctccgagag cgtcaatagc attcaggccc aggacgccgc tcaagccatt gcactcgcgc
     2101 gccgccacag ccccgattgc atcctggtcg atgccgacgc ctcggaagag aacgagcgcc
     2161 tcctcgacgt gctgctcgcc gatgccacca tccaaaccct gccgatggtg ctggtcacca
     2221 acgacgagga tctgtttgaa aaataccgat cgttcatctc tgcgcgcctc aagcgagaat
     2281 tccggaaaag tacgctgctc agcagcattc acaacgccgt gcggcaaccc atcgtgccgg
     2341 aatcgtctct gggaggaaag atcctgtgcg tggatgatga cccggacgtg ctggtttttc
     2401 tgcggcgttg cctggagacc gacggctacg aaatcgatga atgcgaatcc ggagaggagg
     2461 ctctggctcg cctgcgctcg agggaatatg cgctggtcct cctcgatatc gccatgccgg
     2521 gcatggacgg atgggagacc tgcctgcgca tcaagtccga ccccgctctc gcgggtatca
     2581 aagtattcat ggttacagca aaaccccttg acgccaagaa tccgctcacc aaaagctccg
     2641 ggtgcgacgg ttttatccag aaacccttcc gcgcagaaga catcgtggaa atcgcttcca
     2701 gactgctgaa gcggccgcac gcaatcagaa gggcataggc cgtggaagat cctctgcggc
     2761 tcgcgggcct ggcgtccaat agaccttttc gtgccttcgt gcatttcaac cgcccttatg
     2821 cccgggcgta cgtcatcggt gcagcggccg gcctgttttt catcaccatc gacctgtgta
     2881 tgccgatggt ggtccgggcc gtcgtgcacc ggcttgagaa ccggactctg gattaccgtt
     2941 ttctgctgac ctgcgtcgcc ttcctgctcg ccattcccat ctgctccggc ctggcccgtt
     3001 ttgtccagcg catgctcatc atcaaggctt cgcgtgaatt cgaatacgat ttgcgaaacg
     3061 tgttctaccg gaagatccag cacttgtcgc aggagttttt ccattcgttg cgcaccggcg
     3121 acatcatggc ccgcgccacc aacgatctga attatgtgcg catgttcgtg ggacccggtg
     3181 tgatgaacac ggtgaacatg attcgcctgc ctttttcact ggccgcgatg atgtacctga
     3241 gcccaaaatt gaccgggatc gcgctaatcc cactgccgtt catctcattt gtggtctatt
     3301 ttttcatgat gtacatgcac cggcagtcaa aacgtgtgca ggaacagttc tccacggtta
     3361 ccgcgcgcgc tcaggaaaat cttgccggcg cccgggtggt acaggcctat ggcatcgcgg
     3421 atcgggaaat ccgtgatttt cgtaacgaat cccggaaata catgcgcgaa agcctcaagc
     3481 tcacgatcat catggcgctg gcctggccac taatcggaac gaccatcggc gtatccgccc
     3541 tgattattct gtggacgggg gggcacatgg tcatatcgca aacgctcagc ctggccgacc
     3601 tctcggcctt cacggtatat ctcgtgatgc tcacgtggcc gctcgtggag ttcgggtggg
     3661 tattgactct ctaccagcgc ggcgccgtca gcatgtcccg catcctcgag atcctcaccc
     3721 gcaagcctgc cgtcagcgat tccgcgcaaa ctgaccgcag catcaggtcc gttgacggcg
     3781 cggtcgggtt tcacggcgtc tccttcgcgt acaacgggca gaacgtgctc aaaaatatct
     3841 ccgtcgacat ccccgcggga cagaccgttg ccatcgtcgg accaaccggc tcaggcaaat
     3901 caacactgct gagcctgctg gcccgagagt atgatccgca gctcggcgca gtgctaatcg
     3961 acgggcgcga cgttcgcgag tttccgcttg agactctccg gaagcacatc gggtgcgtgc
     4021 ctcaggatcc gtttttgttt tcggattcga tccgcgccaa cctgacgctc gggtgtcctg
     4081 aggcttctca tgaagatttg cattgggcct gtgaaatcgc tcaattcagc gataccctgc
     4141 aggaattgcc caacggctac gatacccttc tgggagagag gggcatcaat ctctccggcg
     4201 gccagaaaca gcggctcacc attgccaggg cattgatctg caagccgcgg attctcatcc
     4261 tcgacgacgc cctgtccagt gtcgataccc atacggaaga agagatcctg cgccggctga
     4321 agcgcgtcat gcgggaacgc accagcatca tcgtatcgca ccggatctca accgtcagcc
     4381 atgccgatcg cattctggtg ctcgacgacg ggaagattgt ggaagagggc acacacgagg
     4441 agcttgtcgc ccgcggcggg ctctatgcgg agatgcaccg ccgtcaattg ctcgaaaagg
     4501 aaatcgagca ggcatgagcg aaggatacca tatcgaggat gaagtccagc agcgcgcgta
     4561 cgaccgccag ctgatgcgcc gtctgctggg ctatctgcgc gcgtaccgct ctgccatgat
     4621 tctcgcagta gtgctcctgt tgggcgcctc gatcctgacc aacctcgttc cctatttcaa
     4681 taagctggcc ctggataaat acatcggaaa tccggagcgg atcgcccagc aggagcaggc
     4741 ggccggcggt acgccttcgg caacactcga agcactcgtg actcacgatc ggcagggtct
     4801 gggcagggtg atcctgatca ttgcctgcct gatgctgggc gagaccctgg cccgctacct
     4861 gcagaatatc atcgtggcct atgtcggcca gaagacaatg ctcgccatgc gcctccagat
     4921 ctttgagcac ctgcagcgca tgtcgctgcg cttccttgat cgcaatccta tcggccgact
     4981 gatgacccgc gtcaccaatg acgtggagaa aattcaggag accatcgtcg ctggaatggt
     5041 tcaggtgatc agcgatttac tcacggtttt tgtcattctg gcgttcatgc tgtgggtaaa
     5101 ctggcaactg gcgctcattc tcatgacaac ggtcccgttc gtcttcgtca ccggcttcgt
     5161 atttcgccgg tatgcccgcc gttcctacat tgagatccgt aaacgcctcg cccgcatgaa
     5221 cgcctacgcg caggaaacaa tcagtggcat gcgcatcgtt cagatgttca cggcagagga
     5281 acccacctac gaggaatacc gaagaagaaa cgaggagcac cgcgacgagt ggtttcagca
     5341 agtcctcaat tacgctgtct acttccctgt ggtggatttt ctcggcatgt tggccatcgg
     5401 tctgatcgtc ctgtacaccg gcgaccggtt tctgcgcttc ggggacgccg tgggggtgtc
     5461 cgtgggcacc ctgttcgcct acattcaatg gtctgaacgg ttttatgctc ccattcgcac
     5521 cattgcggac cgttacaacc tcctgctcga agccatggcc tcgtccgagc gtatctttga
     5581 gcttctcggg gcagaagaag agatagaaaa cgcaccggac gccgtaatcc ccgatcgttt
     5641 cgaaggccgg gtcgagttca aggatgtctg gttcgcctat gagcccgacc actggattct
     5701 gaaagggata aacctcgcca ttgccccggg agaacgtatc gccattgtag ggcataccgg
     5761 ggcagggaag accaccctga tcaacctgtt gagccgcttc tatgacgttc agcgcggcag
     5821 cattgccatc gatggccttg atgtgcgctt gtatgaaaaa accgccttgc gccaacgcat
     5881 cggcacagtg ctgcaagatg tctttctctt ctccggctcc atcgaacaaa atatccggct
     5941 cggcaaccag gagctgacca tggagcgcgt gcgccaatgc gcggcctatg tcaacgcggc
     6001 ccgatttatc gaaaaaatgc cgggcggcta tgcctacgac gtaggggaac ggggcaataa
     6061 cctgtccacc gggcagcgcc agttactggc ctttgcacgc actctggccc acgaccccca
     6121 actccttgtg ctcgacgaag ctacgtccag cgtggatacg gaaacagaag tcctcatcca
     6181 ggacgccatc cgcaaactca tgctgggcgg tacctccatc gtcatcgctc accgcctgtc
     6241 cacagtccag aatgccgacc ggattatcgt catgcatcat ggcgaggtcc gggaaaccgg
     6301 cacgcatcgt gaattgctcg cacaacgcgg actctattac cggctgtatc agctgcagta
     6361 caagcaccag aacggcactt ccgaaaccac acaaattccc tgaagtgctt atttcgcact
     6421 ggcttacgaa gggctttgca gtcttcatct gagggcgtat ccgcccgggg cgcatcacaa
     6481 accgccaccc ctcaggaaac actggcgccc gaacgcgggc cgcctggtct acacaaacag
     6541 gtccgcacca tggcctcgcg agcttcccac atttccacag tcgcgacggc tgttgcacac
     6601 gacagacggc ttgtgaacag ccggcggagg caagcgcctg cccccaccag cccacaagcc
     6661 ttggaaaact cgcccagcga ctaacccgaa aaccacggca acgtgacatt tctaacgagc
     6721 taagacatgt gacattttta aagagtcttg acacacgcaa atttttaact tgatctgagg
     6781 gctcaacatc attatcatca ctcaactcct ttgggcgcct ttcgcgcagt gctcccggtg
     6841 gagcagaccc cggttggcat ggccgataac gggcacccaa gcaacatacg gacgatgaga
     6901 gctgaaggac gggtatatcc gcacgtaaac cagacggaga gtacgatgct tgaattcaag
     6961 aaaggcgtgg acattctttc cgccattggt gggatcgact gtattgagaa agccagggcg
     7021 ctgttcaaag aacaactgga tgctgaacac cttacgaggc tgtccatcat taccaatgaa
     7081 gaagtcctgc tgaaggttgc caatgcgata gccatgtgtc agccggacag ggtctatgtg
     7141 ttgaccggtt ccgaagccga caaggagttc gtgaagaagt tcagcataga gaaaggcgag
     7201 gaaacgcccc tggcaattcc aggccacacc tatcacttcg atctacccga ggaccagggc
     7261 cgcctgctga atcagacctt gtatatcgtg aacgaggatg aacagatttc gtcgttggcc
     7321 cagcgcgagc cgcgcaacga atcgcacgcg cacatcgaaa aatacatgtc cggcatcatg
     7381 aagggcaaga ccatgttcgt cgggttctac tcccgcggcc ccattggcgc ccaagcctcc
     7441 attccggcca ttgaaatctc gagttccacg tacgtgttgc attccgccgc actgctgtac
     7501 cgcaactgtt acgcggaatt cgacgccgaa gtcgagcggc gcggcgaatt tttcaccaac
     7561 gtccactcgg aaggccccaa tcgtccggaa gacattccca attgccgcat ctacatggat
     7621 cgctcctggc agacgaccta ttcgatgttt tgcacctacg cgggcaacac gctgctcatg
     7681 aaaaagggaa accaccggtt tgcgtccgat tcggccatct acagaaatta cgggcgtgaa
     7741 ctctccgagc acatgttcat taccggtatg acgggtcccg gcggccggaa aacgtacttt
     7801 gccggtgcgg cgccgtccgg atgcggaaaa accaccaccg ccatggtcgg ctcagatttc
     7861 gtcggcgacg acctggccca gctgtggatc ggggaagatg gcagcctgcg cgcaattaat
     7921 cccgagatcg gcattttcgg catcctggaa gatgtgaact acgctggaga cccgcacctc
     7981 atgaagtgcc tgcgcgaacc aggcgccgaa gtcatttttt ccaacgttct catcgacgcc
     8041 gacaagaaac cgcgctgggt gggcgacggc gagcccgtac cggaacgggg cttcaatttc
     8101 cagggcgaat ggacgcccaa catgaccgat gtccccatgt ctcacccgaa ttcgcggtgc
     8161 acgcttctgt cggaagccat cgcgaatcac aataaagaag ccaacgccga cccgtccggt
     8221 gtgcagatcg ccgtggtgac ctattccggg cgggattccg acaccatgcc cccgatttgg
     8281 gtggccaaga ccatggccga cggcgtagcg atcggcgcat ccatcgtctc gaaagccacg
     8341 gctactgaaa tcggcgcctc cggcattcgc cgccagccgt gggccaatgc ccccttcatc
     8401 gccggtaaac tgggtgatta cctcgacgct caatttaaag tcttcaccaa ccccgatctg
     8461 aaacaaaagc cggtgatcgc cggcctgaac tactttctca cctgcgaaaa ccggggtggg
     8521 ggcggcgccg gactgctcgg tgagaagaga gacgtgcatg tatggctcgg ctggctcgag
     8581 ctctacgcgc acggcgatgt ccaggcactc gataccccca tcggcatgct gcccaaatac
     8641 gaggatctca aggccctttt caaggaaaaa ctcgacaaag agtacagcga agagctgtac
     8701 acaaagcagt tctcgattta tgtcgacaac gtcctgatgc tcattgacct cgagattgcg
     8761 gcctggcaac aggaagagag cgtcggtcag accatctttg atatctacgc aaaacagaag
     8821 gccggcctcg aagcgctgaa agccgccaaa ggacccgtcg tcaggcccca ggatttttag
     8881 acgcctgccc tgcacaacaa cccccgcccg ccccggataa accgcggcgg gcggtatttt
     8941 tgttctgccc gatgagggga tggggtacgc atttcaaggt gccgggaatt tcgtgaaata
     9001 tccgtcacct cagtgcataa cgtcgtggaa tgactaaggc ttaagaagat gttcggcaaa
     9061 ggtcagacca gcaaagccat ctaagaagcc ccactggtca cgattttgga gtgggcacga
     9121 acattgtccg cgcccgtcat gaggccgcaa gccttgcact caatttctgg cgctgtgccg
     9181 caatgacctc ggtcatttcc tgagccgaaa ttgccgcagg cgaactctct tttaagagac
     9241 tgtcccgcgc atagtcatcc atgtagcggc gcttctgggc caggctctca acgatccctt
     9301 cctcaatggt atgcagggat gtgagccggt gaacattgac actccggcgc tggcccatcc
     9361 gatgtacacg gcagatggcc tgatattcag ccgctgggtt caattgcggc tcgaccagga
     9421 ttacggcaga tgcagcctgc atgtttagcc caaccccgcc ggccccgatc tgagccagaa
     9481 ttatcttgcc cgtgggggaa ttctcgttca actcatcgat tacgttcatt cgttctgaag
     9541 tcgaaacagc tcccataatc acacccgcat cagggaaact gacgctcaac gtatctatca
     9601 ctcttttaaa ataggtgaac accaccacgc tccgcccatt ttcgacatac tcctgaatca
     9661 gttcctgcac acgggcaatt tttggagatt cgtccccatc cccaaagatt gcatgctgcc
     9721 tgagatgcat aaaatgggct ccagcgttca tcatcgcaag atgtgacttc aggtcagctc
     9781 tatctaacac tatgctctcg ttgatctcgt tgaggtcagg caattcgttt aacacatctt
     9841 ccttatttcg tcgcagatag agatctttca ccgcacgttt gaagtagtcg ggagaccccg
     9901 gatttcttga ggctgtcatc cttgagactg tcgcattccc atgacgcaat cctctggtga
     9961 tggccagaaa ctcttcggga tgattctcaa tgggcgtacc cgtcatggca agcgcgtacc
    10021 tggcttgggc gaacactgct ctcgtagctt tggcgcgttt agtatgagga ttcttaatgt
    10081 attgggcctc gtctgcaaca acgaagtcca ggaatcgcat tctgggcatg agagctttgt
    10141 gttttccccg cagtgtgtcg taggagatga tgccaattcc gcctttagct tgccacgcat
    10201 cgacatcttg ttcggtctgc gacggccgta tcacaaaggc gttcagccgg gttttgtcat
    10261 ccacctcccg gagccagttc tctcggaggc ttgccggaac gataactaat gcacggattt
    10321 ttccgttgca agcctccaaa tgggcgatcg cagccagggt ttggatagtc ttgccgagac
    10381 ccatttcgtc gccgagaaag cactgtttcc ggacaaggat gaactttgca ccaaaatcct
    10441 gataacgccg aagcaggcaa ttctgcaagc cagaagtgtc gagggtaaat gcctctattt
    10501 tctttgtcag gtttgctggg atgccgccct cccagggtgc tgacgtgcca tcctctcctg
    10561 cctggggata ccggcgttga agcaattcga tggcacgtat gtaatcattc tgggaaagcg
    10621 tggagtaatt tgccttttcg cctctgtccg tatcccggac aaaacttaca attctatcta
    10681 acgcatccat tgccgcgtca gtttgttgcg cgcttgttcc tcgccgtcca aacatatatt
    10741 ggataaaaga aattgtcggg aactgccggg caagtgctgc gatttgcata acagcacgat
    10801 ttgccgacat tgagacgcca gtcagccagt cgtggtcgat aatgagttca atgagccgcc
    10861 gaacctcctg cttgtcgacg gccagcttga ccggttgcga gcgtgcggaa gctgcggcct
    10921 cttgaacgta ccgtgcgatc cctacttttc cacgctgtga aacgccctgt agattgtgga
    10981 ctatgtggcg ccgggcgtga agatccagca gattgtgggc acccgcttct tcaagcttgc
    11041 gtagcgtgac ggggccaatg cccgggattt ctctgagttc atccagcggg cgcgcggcaa
    11101 gatggcgctg aaactctgcc gtcttatgcg ccacccagag ttgatcgagt tgagactcaa
    11161 ggttggcttg ttccgcctgc agcctgccgc aaaggtcttg gatgcgctgt ctctcggcat
    11221 cgatgtgtct ggccctggca atataggggt cgattcgata ctcaccaaag tctccacgcg
    11281 tgagtctatc cattaatttg gtcaggaacg ggggccattt atcgcgcggc ggcgtgaaaa
    11341 tgcccccgca ctgaaggcac cggccttgta agccaccgta tttatcaggg atgtacagca
    11401 acttatggca atgtgggcat gcgtgataca tcgcgcaggt tcctcccaga agccttcccc
    11461 gcaggtgcat actcctcgaa attttgcgaa tgcacctcag gacataatgt caaaactcgc
    11521 aaaaattgtc acctccctat ctcaaatgct acgccgctgc aatttgcacg ttatacacag
    11581 cagtctggca cggacttcga caggcggtct ccttagccag acgcagttct tgtaccggga
    11641 aaggcgtctt gctatggttt tgtcgaagat catacgggga gggcctgttt gtgtcggata
    11701 agggattgtt cctgcagaat gtgatagcgg tgatctggga ttttgaccgg acgttgtcgc
    11761 cgcagtacat gcaaaaacca ttgtttgagg cctacgacgt cgatgagaac accttctggg
    11821 agcaggttgg gcaattgccg gcctactatc agcggtcggg cattcatgtg cagcgggata
    11881 cgtgttatct cggccacatc ctgtcgtatg ttcgggcggg gcgttttcag ggcctcacca
    11941 acgccaaatt gcgggatctt gggaaaaaca tcccatttta tcggggattg ccgttttttc
    12001 tcgaccggct caaggagatt gtgaaggaac ccccgtatgt ggagggcgac cttcgcctgg
    12061 agcactatgt ggtcagcacg gggctggcgg agatcatccg cggctctgaa atcgccgaga
    12121 aactcgacgg cgtgtgggct tcggagttca ttgaagcccc cgccgagccg gacaccgacc
    12181 tcgcaggcaa ccccaggtcc ggggagatct ctcagatcgc gaccctgctg gataacacga
    12241 ccaagacccg ggcgctgttc gaaatcaaca agggcgtgaa caaagagccg agcatcacgg
    12301 taaacgacgc catcccggaa gaggagcggc gagtgccctt taaaaatatg atctatgtcg
    12361 ccgatggccc aagcgatatc ccctcgttct cggtggtacg gaagcatggg ggactcgcct
    12421 tcgccgtcta caagccgcgt tctcaacggc attacgagca ggcgctgatg ctccaggaaa
    12481 ccgaccgggt cgatatgatc gggccggccg actaccgtga gggggcctcg accgacatgt
    12541 ggttccagtc gcagatccgc cgaatcgcga cccgcatgct tgatgagcgc cggcgggctc
    12601 tggagagcaa ggtccgcaag gaacccgtgc acctggccga aattcagcgg cagaagaaac
    12661 tgaaagaaaa agaagcgggg gagtagcgcc tcagatgcag tcgcccggcc agtctggcgg
    12721 tttgccctca aacacggcga aatgccggcg cgggtcgctc aggaggcgtt tcgcttcggc
    12781 aagctggccg atgccgcggt gccgcgcgtt cacacccgcc acgaccgagc cgcaggccag
    12841 cgcggtagcc ggttcatccc agcgcagcat ccccgccaga aatcccgcgg agaagctgtc
    12901 gccgcatccg gtcgtatcca cggccttcac gg
//
LOCUS       DAOPBQ010000032        12830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4857_length_12830_cov_4.025440, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000032 DAOPBQ010000000
VERSION     DAOPBQ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12830
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4857_length_12830_cov_4.025440"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /gene="der"
                     /locus_tag="PKW60_02100"
     CDS             <1..1279
                     /gene="der"
                     /locus_tag="PKW60_02100"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPJ98254.1"
                     /translation="TLFNRIVGKQRAIVHGEEGITRDRTYATAQWGERAFRVVDTGGV
                     VENPLDPVSHKVQIQVREALQEADAIILVVDGQQEITRTDEELRDELFKYGKPVFVAV
                     NKLDNERLQLHLAEFYALGLGDPHSISATHNMGIESLVDAVLRVLPERSQTTVVEVDA
                     TRIAVIGKPNVGKSSFVNAILNTERSIVDATPGTTRDAIDIDVVWNDRPYVLIDTAGL
                     RRKSGIKEDVEHFSISRSLRAIRRADVCLVLIDATEGISEQDKRILAYVQENGAGMVL
                     VWTKWDLIEDKELRFKELSDEIDLKAPFLRYVPSITVSNTTRQRLFKIFDLADLVAAE
                     AEKRIPTPELNRLFEQLRSEEVSGTYKGKRAKVLYATQTGTKPAKFTLFVNQKRLFHF
                     SYLRFIENRLRERFGWQGVPIHIELREETRGKS"
     gene            1276..1899
                     /gene="plsY"
                     /locus_tag="PKW60_02105"
     CDS             1276..1899
                     /gene="plsY"
                     /locus_tag="PKW60_02105"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPJ98255.1"
                     /translation="MMWQTAAAIGISYLLGSIPTGLWFGKALRGIDIREHGSRNIGAT
                     NTLRVLGKKLGALALAGDLGKGLVAVLLVAPLLSRWPYAPLACGLAAIIGHVAPVFLK
                     FKGGKGVATSTGVFLALAPPATLFAAAVFGAVVYATRMVSAASLSAATALLVAVYALP
                     HAWATCPTHAIPPGWALRGVATIVALLVLVRHRTNLQRILKGEENRF"
     gene            complement(1925..2323)
                     /locus_tag="PKW60_02110"
     CDS             complement(1925..2323)
                     /locus_tag="PKW60_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98256.1"
                     /translation="MPRLLAAFLLALCGFGCAHDTGSPPVFTKPWYDKTQAAMTEDKA
                     RKEAQGVLLGDENNAVSLRADEEGRPRLQIGKDRVHADLDVKSGDPRVRLRYKLQWGH
                     GEKDMPEHIPRLHPAPESPPESGQPATEDK"
     gene            2517..3656
                     /locus_tag="PKW60_02115"
     CDS             2517..3656
                     /locus_tag="PKW60_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018618075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPJ98257.1"
                     /translation="MAVWAVIVALASAAEEAPLLRFEKTRIGDVVYEAAGACDINNDG
                     VMDIVSGGCWFEGPDFTKSHKICDVDAVGDYFDDFADIPLDVNGDGFVDIVSGAWFSK
                     KLTWRENPKGEPGEWTVHDIAEVGNIERPCCFDIDKDGHLEILPNTPGGPLRAFKLVR
                     DANGKGTGKFDEFVLFNENSGHGLGFGDINGDGRPDIVLARGWLEAPESPFSGEWVLH
                     PEFDLGPSASVPILVHDVNADGLNDLIVGAGHDYGLSWYEQQRDSDGNRTWIKHEIDT
                     ERSQYHDLQLADIDNDGQLDLITGKRYRAHSGQDPGADEPVGIYYFKIDGGQFVRKTI
                     DYGDPKECSGAGIYFWVADIDGNGWKDVIAPGKEGLYLFRNLGAM"
     gene            3776..4510
                     /locus_tag="PKW60_02120"
     CDS             3776..4510
                     /locus_tag="PKW60_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010198925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HPJ98258.1"
                     /translation="MPRNTQIRPQYETELAQPVTAFLESQGYTVRSEVNGCDIVGVRG
                     GQLVVVELKRNFTVTLLAQAADRQRVADAVYVALPRPAESLRGKRWRILRHLLRRLEL
                     GLLFVSFTTEKPRVEVVFHPVPFDRKRDHRRRQGLLRESGGRSSDFNQGGSARRKLVT
                     AYRENAIRIACALEALGESSPAALRALGTGPRTRAILYDNVYGWFERVDRGVYRLQEA
                     GREALADYPDLATHYRKEIDQKRSAR"
     gene            4658..5119
                     /locus_tag="PKW60_02125"
     CDS             4658..5119
                     /locus_tag="PKW60_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HPJ98259.1"
                     /translation="MKIIVGADAWGVELKDAVKQHLVKHGHEVTDVGGSPGNDRPYYE
                     VASEVAGMLQAGAAERAVLVCGTGMGMAIVANKFKGVYASVIESEFTARMCRAVNNAN
                     VLTMGSMVVAPYVAMQAVDAFLATKHTEGLEDVADFLHTALNAVAQIEQEA"
     gene            complement(5157..5822)
                     /gene="pgl"
                     /locus_tag="PKW60_02130"
     CDS             complement(5157..5822)
                     /gene="pgl"
                     /locus_tag="PKW60_02130"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPJ98260.1"
                     /translation="MSEYRVCADPVRSFAEAFAEKMAQLERPFVALSGGCTPRPLYRL
                     WAREYRCSLPWDRMHIFQVDERCVRADSDASNWKLLHESLLWAVPEARAYPMEAERDG
                     AAEAYEQLLRNRVPAGEDGIPRLDLVLLGLGADGHTASLFPGTQAALERERLVVRTDT
                     PGIEPARVTLTFPTLESAGQRWFLVLGSAKAQAVAAAVAGAHPAGKLDAIWFLDAEAA
                     SEI"
     gene            complement(5841..7385)
                     /gene="zwf"
                     /locus_tag="PKW60_02135"
     CDS             complement(5841..7385)
                     /gene="zwf"
                     /locus_tag="PKW60_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HPJ98261.1"
                     /translation="MPGRATTHFMHPPERDRSGFIIEPAAMATMVIFGATGDLAERKL
                     IPAIYNLWNAGYLPRRIAVVGAARRPLDENDFRQSMCEALKKHSRTGHGDSDRCDPFV
                     SNLYYQQVRFDVPDDYARLKVRLDAIEAEQGAPGNRLYYLAVAPEYFATIVEQLGPSG
                     CVHTPGCTPWTRVIAEKPFGFDLESARQINRMIGAVLDESQVYRIDHYLGKETVQNIL
                     AVRFGNAIFEPLFSQKYIDHVQITVAETIGMEGRRGAFYDTVGALRDVVQNHALQLLC
                     LVAMEPPARFNARYIHNEKLKVLNSIEVPLDEPLDQWCVRGQYGEGDGGPAYLAEEGV
                     DPRSCTESYVALRLFVDNWRWAGVPFFVRTGKRLQKRLTEIMVQFKDPPMHLFRELGA
                     TLPETNVLVFRIQPDEGISLTFNAKPPGMDFAVRPVAMNFAYGHTFDEAIPEAYERLL
                     LDAIRGDSTLFTRADEIESAWDIVTRIREAWTGAGPPVTYSPGTWGPAEAERLFGNCR
                     GAWHNP"
     gene            complement(7488..8792)
                     /locus_tag="PKW60_02140"
     CDS             complement(7488..8792)
                     /locus_tag="PKW60_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPJ98262.1"
                     /translation="MAKTKAKPKTKAISRAKKAAPGTAKKLAKPAKTTVKQHPAPKGT
                     SKAIAKTKKKPSANKPKTGPETKIKATMKKKTPAPAKAAPKKAVETKAQQEAPAPAKS
                     VAARTKGSLLDRDFLIALAEAIRVVVQPAIDASKGREVIGNAASGDATFQLDRLAERA
                     LLTFLRDAKLPVAYYSEDSGYTTFTSGQPEHLLVIDPIDGTRAAKSGFEWCVIAIGST
                     RVIERPRLGDIDNALVMELMGSRAFYAERGKGARVYVDGQYRKPKLSKNTDLETVVWS
                     MTVPARPAELIFPTAAKLIDLTSLKGGFYACNSTSYSLTRLLTNQLDACVDIANRFYR
                     DIPKAVQDHFINAGRGAILGIAPYDIAASLLIAEEAGCVVTDAYGKSLRDVLLLDSAT
                     GNHQSMVAAANAELHEKLLTFFDMRIQQWEQLLRRHEDRPSA"
     gene            complement(8954..10120)
                     /locus_tag="PKW60_02145"
     CDS             complement(8954..10120)
                     /locus_tag="PKW60_02145"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HPJ98263.1"
                     /translation="MPTVFRLAVEAGPHDRSFCPVSVELPVDYSKNRGLLLKDAKSGK
                     TIPSQVSPHGQGITLSWLVTGLKSGETRHLTAKPVPNASHKPTVTVEDNPGDSVVNVS
                     IFGKPFTAYHYGERWARPFLYPVIGPGGARVTRSWPVSSQVKGEKRDHPHHKSIWVAY
                     GACGPAKVDNWSELPGHGRQRHIRFLKKESGAVFGEIVAKNDWCTNRGRKQFEETRAM
                     RFYALPGGTRLFDLAVTFRMTEGPITFYDTKEGGLVSVRVASSMDVPRTGRIENAYGG
                     INEAETWGKNAPWCDYSGVVDGVHAGIAIMDHETNPRYPTGWHVRDYGLMTANCFAWK
                     HYRPHARMKGDMTFGNGSRTMWRYRLLIHPGDAARGKVKDRFMDFIAPPRVALA"
     gene            complement(10426..11985)
                     /locus_tag="PKW60_02150"
     CDS             complement(10426..11985)
                     /locus_tag="PKW60_02150"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HPJ98264.1"
                     /translation="MHTGRPISRREAFKLSAAGAAAWAAAPYTSTAQAPPGKPNILFI
                     MADQYRADCVGADGNAAISTPNLDRLAREGALFSKAYTSTPSCTPARAGLLSGLSPWN
                     HGMLAYGRVGWKYPRELPQEMRNAGYYTLGIGKMHWSPQRSLHGFHQTILDESSRVES
                     IDFRSDYVSWFYSQAPTLDWHATGLGFNEYDARPYVLPEHLHPTAWTGDTAVNFLANY
                     EREEPFFLKVSFARPHSPYDPPERFWKMYAERDIPKAVVGGWAGKYAQPSGDDPNLWH
                     GDLGPGQVRKSRIGYYGNVTFIDEQIGRMLNILEERGWLENTLILFTADHGDMTGDHH
                     LWRKTYAYEASARIPLIIRWPEGLVRATRGLRLRQPVELRDVMPTFLDTAGAGVPGDI
                     DGDSLLKLVRGETEGWRPWLDLEHGRCYGPDNHWNALTDGRWKYIYHAHLGEEQLFDL
                     DADPSELADLSQTNPAETARWRERMIAHLSVRGEQFVKDGKLVVRENSLLYGPNYPGF
                     WEDAQAAGKTK"
     gene            complement(12031..>12830)
                     /locus_tag="PKW60_02155"
     CDS             complement(12031..>12830)
                     /locus_tag="PKW60_02155"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPJ98265.1"
                     /translation="GNMTAESLQPALSQALFNRPIHVHCAAAEADPATNEYVEQASSR
                     QGVPGLMGFAPDLIAPEALEEALGSKRFAGIVLDSSEDLTAALPEWAMQILDRLGRLL
                     VLDISLSDENTAWQVAELCMQYPRAKFVVESAGKYQTLTDAAQGLYQFKDVLNLFFTT
                     ACVTEWQILESLFAAIEPHRVLFATGGLRALQLGKSVIINDYVVEATPEHTKDSDGDA
                     PPFTLRLYEQIWAIRTAMERLGINRDHILDFFNDNARQLLNVAMRKS"
BASE COUNT         2362 a         3885 c         4010 g         2573 t
ORIGIN      
        1 cacgctgttc aaccggatcg tgggcaaaca gcgcgccatc gtgcatggag aagaaggaat
       61 cacgcgggac cgcacctacg ccacggccca gtggggagaa cgcgcctttc gcgtcgtgga
      121 cacgggcggc gttgtcgaaa atccgctcga ccccgtgtcg cacaaggtgc agatccaggt
      181 tcgggaagct ctccaggagg ccgacgcaat catcctggtc gttgacgggc agcaggagat
      241 cacccgcacg gacgaggaac tgcgtgacga gctgttcaag tacggcaagc cggtgttcgt
      301 cgccgtcaac aaactcgaca atgagcgcct gcaactgcat ctggcggaat tctatgccct
      361 gggcctgggc gatccgcatt ccatatccgc tacgcacaac atgggaatcg aaagcctggt
      421 ggacgccgtg cttcgcgtgt tgccggagag gtcgcagaca acagttgtcg aggttgacgc
      481 cacccgaatc gcggtcatcg gaaaacccaa cgttggaaaa tcatcgtttg taaacgcgat
      541 actcaacacc gagcgcagca tcgtggatgc cacacccggc accacgcggg atgccatcga
      601 tatcgatgtc gtctggaacg accggcccta cgttcttatt gacaccgccg gcctgcggcg
      661 gaagtcgggc atcaaggagg acgttgagca tttcagcatc agccgttccc tgcgcgcgat
      721 ccgccgggcc gatgtctgcc tggttctgat cgacgccacg gaaggcattt cagaacagga
      781 taaacgcatt ctggcctacg tgcaggaaaa cggtgcaggg atggtgctgg tctggaccaa
      841 atgggacctg atcgaagaca aagagcttcg attcaaggaa ctcagtgacg aaatagactt
      901 gaaagcgccg ttcctgcgct acgtcccctc cattaccgtt tccaatacca cacggcagcg
      961 gctgttcaaa atctttgatt tggccgatct ggtggcggct gaggccgaaa agcgcattcc
     1021 cacgccggag ctgaaccgcc tcttcgagca gttgcgctcg gaagaggtca gcggcacata
     1081 caaaggcaag cgggccaaag tgctttacgc cacacagacc ggcacaaaac ccgcgaagtt
     1141 caccctgttc gtgaatcaga agcggctgtt tcatttcagc tacctgcgtt ttatcgagaa
     1201 tcgcctgcgc gagcgctttg ggtggcaggg ggtccctatc cacatcgaac tgcgggaaga
     1261 gaccagagga aaatcatgat gtggcaaacc gcggcagcca ttgggatttc gtacctgctg
     1321 ggctcgatcc ccacggggct ctggtttggt aaggccctgc gcggtatcga tattcgcgag
     1381 cacggcagtc gaaacatcgg cgccaccaac accctgcggg ttttgggcaa aaagctgggc
     1441 gcgctcgccc tggccggcga cctgggcaag gggctcgtcg ccgtcctgct ggtggcgccg
     1501 ttactcagcc gctggcccta tgcgcccctg gcctgcggcc tggcagccat tatcggccac
     1561 gtcgcgccgg tgttcctcaa attcaagggc ggcaaaggcg tcgccaccag cacgggtgtc
     1621 ttcctggcgc tggcgcctcc cgccacgctg ttcgcggccg ccgtgttcgg cgccgtcgtg
     1681 tatgcgacgc gcatggtgag cgcagccagc ctttccgccg ccaccgcgct gctggttgcg
     1741 gtctacgccc ttccccacgc atgggcgacc tgccctaccc acgcgattcc tccgggctgg
     1801 gcactccgtg gagtggccac catcgtggca ttgctcgtgt tggtccggca ccgcacgaac
     1861 ctgcagcgca tcttgaaagg ggaagagaac cggttttgaa ccgcagcgca tccgcccggc
     1921 ccggtcattt gtcttcggtg gcaggttgcc cactctccgg aggagactcc ggcgcgggat
     1981 gaagccgcgg aatatgctcg ggcatgtcct tttcgccatg accccactgc agtttatagc
     2041 ggagccgcac ccgggggtcg ccgctcttga cgtcgaggtc tgcgtgaacc cgatctttcc
     2101 cgatctgcag cctgggccgc ccttcttcgt cggcgcgcag ggacacggcg ttgttctcat
     2161 cccccaggag gaccccctgg gcttctttgc gcgctttatc ctcggtcata gcggcctggg
     2221 tcttgtcgta ccacggtttc gtaaacacgg gcggcgaacc ggtgtcgtgc gcgcaaccga
     2281 aaccgcacag agccaacagg aaggcggcca ggagccgggg catcggcccc tgtttcttac
     2341 gagagggatg tgccgcagcg ttcatcgtga taattatacc ttcaagacct tgaaacctta
     2401 ctatggaatc ttgagtgtgc gcgttcccgg ccattccgcc ccgcgagaaa tttccccgga
     2461 cggcccgctt ctgctaggat aatcctctca gccaagtgca atacaggaga tacatcatgg
     2521 cggtatgggc agtgattgtg gcgctggctt cagcagcgga agaggcgccg ctgctgagat
     2581 ttgagaaaac acggatcggt gacgtggttt acgaggcagc cggcgcatgc gatatcaaca
     2641 acgacggcgt gatggatatc gtctcgggcg ggtgctggtt tgaaggcccc gatttcacga
     2701 agagccacaa aatctgtgat gtcgacgcgg taggcgatta tttcgacgat tttgccgaca
     2761 tcccactgga tgtcaatggc gacggctttg ttgacatcgt cagcggcgcg tggttcagta
     2821 aaaagctgac ctggcgcgaa aatcccaaag gcgagcccgg cgaatggaca gtccacgata
     2881 tcgccgaggt cggaaacatt gagcgcccct gctgttttga catcgataag gacggccacc
     2941 ttgagatctt accgaacact ccggggggcc ccctgcgtgc gttcaagctc gtcagggacg
     3001 ccaacggcaa agggaccggc aaattcgacg aattcgttct cttcaacgag aacagcgggc
     3061 atgggctcgg attcggcgac atcaacggcg acggccggcc cgacattgtg ctggcgcgcg
     3121 gctggctcga agcgcctgaa agccctttca gtggcgaatg ggtgctccat ccggagtttg
     3181 atctcggccc ctcggccagc gtgcccatac tcgtccatga tgtaaacgcc gacgggctca
     3241 atgatctcat tgtgggcgca ggccacgact acgggctgtc ctggtatgag caacagcgcg
     3301 acagcgacgg caaccgtacg tggatcaagc acgaaatcga caccgaacgt tcccagtacc
     3361 atgatctgca actggctgac atcgacaacg atggccagct cgacctgatt accggcaagc
     3421 ggtaccgcgc acattccggc caggatcccg gcgccgatga gcccgtgggc atctactatt
     3481 tcaagattga cggcggccag tttgtccgca agaccatcga ctacggggat ccgaaggaat
     3541 gcagcggcgc gggcatctac ttctgggtgg ccgacatcga cggaaacggc tggaaagacg
     3601 tcattgcgcc cggcaaagaa gggctatacc tcttccgtaa cctgggcgcc atgtaatctc
     3661 atcggaaacc gcggcgccag gatggatgcg ccgtctggcg gcgcggcgcc cggttttttc
     3721 gagacagcaa ggaagatatg cgccgtatcg ccggcgacgt gaaaacgccg cagtcatgcc
     3781 ccgaaacact caaatccggc ctcaatacga aaccgagctg gcacaacctg tgacggcctt
     3841 tcttgaatcg cagggttaca cggtgcgaag cgaggtgaac ggctgcgaca tcgtcggggt
     3901 ccgtggcggc cagctggtcg tcgtggagct caagcggaat tttacggtga cgctgctggc
     3961 ccaggccgct gacaggcagc gggttgccga tgcggtctat gtggcccttc cccggcccgc
     4021 ggaatccctg cgtgggaaac gctggcgcat actccgccac ctgctgcggc gcttggaact
     4081 gggactcctt ttcgtgtcgt tcaccactga aaaaccgcgg gtggaggtcg tgtttcatcc
     4141 cgtgcctttt gatcggaagc gcgaccaccg gcgccggcag ggtctgctgc gagagtccgg
     4201 cggccgctcg agcgacttta accagggcgg cagcgcccgc cgcaaactcg tcaccgccta
     4261 ccgcgagaat gcaatccgta tcgcctgcgc gcttgaagcg ctcggcgaaa gctctccagc
     4321 agcgctgcgc gcgctgggca ccggtcccag aacccgcgcc attctctacg ataacgtgta
     4381 cggctggttt gagcgtgtgg atcggggggt ctaccgcctt caagaagccg gccgcgaagc
     4441 gctggcggac tacccggacc tggccacaca ttatcggaaa gaaatcgacc agaaacgatc
     4501 ggcgcgctag tgcgcccccg ctcttttcac gggctgggcg gtttattcgg tttcacggtc
     4561 ttcgcgctga gaatttgtgc cggaggatcg tgtatggtgt gggggttccc gccgtggtat
     4621 tcaggcggca aagcagcaga ccggaaagga tagacggatg aagatcatcg tgggcgcaga
     4681 cgcgtggggc gtcgaattga aagatgccgt gaagcagcat ctggtcaaac acggccatga
     4741 ggttaccgat gtcggaggtt ccccgggaaa tgaccgcccg tattatgagg tggccagcga
     4801 agtggccggg atgctgcagg cgggagcggc ggagcgggcc gtgctggtct gtgggaccgg
     4861 tatggggatg gcgatcgtcg ccaacaagtt caagggggtg tatgccagcg tgatcgaaag
     4921 cgagttcacg gcgcgcatgt gccgcgcggt gaataacgca aacgtcttga cgatgggcag
     4981 catggtggtg gccccgtatg tcgcgatgca ggccgtcgat gcctttcttg cgacgaaaca
     5041 tactgaggga ctggaggacg tggcggattt ccttcacacc gccctgaacg cggtcgcgca
     5101 gattgaacaa gaggcataag cgcccgaacg cttcgattca ggcattccgg cagcgcctaa
     5161 atctccgagg ccgcctccgc gtcaagaaac cagatggcgt cgagttttcc ggcgggatgc
     5221 gcgcccgcga cggctgccgc gaccgcctgg gccttcgcgc tcccgagtac caggaaccag
     5281 cgctggccgg ccgattccag cgtgggaaag gtgagcgtaa cgcgggcggg ttcaattccc
     5341 ggcgtatcag tccgcacgac caggcgttcc cgttcgaggg cggcttgtgt gcccgggaac
     5401 agtgacgcgg tgtgtccgtc ggccccgagt cccagcagca caagatcgag ccggggaatg
     5461 ccgtcttctc cggcgggcac gcggtttcgg agcagctgtt cataggcctc cgcggccccg
     5521 tcgcgttcag cctccatcgg atacgcccgc gcctcgggca ccgcccataa aagggactcg
     5581 tggaggagct tccagttcga ggcgtcgctg tctgcccgca cacagcgctc gtccacctga
     5641 aaaatgtgca tgcgatccca gggcaggctg cagcggtatt ccctcgccca gagccggtac
     5701 agcggccgcg gggtacagcc tcccgacaga gcaacgaagg gccgctcgag ctgggccatt
     5761 ttttcggcga acgcctccgc aaaggagcgt acgggatcgg cacagacgcg atattcactc
     5821 atactgacac ctccgttggg ttaggggttg tgccaggcgc cccggcagtt cccgaagagg
     5881 cgctcggcct ccgccggtcc ccacgtgccc ggggaatacg tcacgggcgg tccggcgccg
     5941 gtccaggctt cgcgaatccg tgtgacaata tcccaggcgc tctcgatctc gtctgcccgg
     6001 gtgaacagcg tgctgtcgcc ccggatggca tccagcagca gccgctcgta cgcttccgga
     6061 atggcctcat cgaatgtatg tccatacgca aaattcatcg ccacgggccg gacggcgaaa
     6121 tccatgcccg gcggtttggc gttgaaggtg agggaaatgc cctcatcggg ctgaatccgg
     6181 aacaccagca cgttggtttc gggcagcgtg gcccccagtt cccggaacag atgcatgggc
     6241 ggatctttga actgcaccat gatctcggtg agccgcttct gcaggcgctt gcccgtgcgt
     6301 acaaagaaag gcaccccggc ccagcgccag ttgtctacaa agagccggag cgcgacgtag
     6361 ctctcggtgc atgagcgggg gtcgacccct tcttcggcca ggtacgccgg tccgccgtct
     6421 ccttcaccgt attgcccgcg cacgcaccat tggtctaaag gctcatccag aggcacctcg
     6481 atgctgttga ggaccttgag cttttcattg tggatatagc gggcgttgaa ccgcgcgggc
     6541 ggctccatgg ccacgaggca cagcagctgg agcgcgtggt tctggactac gtcgcgcagg
     6601 gcgccgaccg tgtcgtagaa cgcgccgcgc cgcccttcca tgccgatggt ttcggcaacg
     6661 gtgatctgga cgtgatcgat gtatttctgg ctgaacagcg gctcaaaaat ggcatttcca
     6721 aaccgtaccg ccagaatatt ctgcaccgtc tctttgccga gatagtgatc gatgcgatac
     6781 acctggcttt catcgaggac ggcgccgatc atccggttga tttggcgggc cgattccagg
     6841 tcaaacccga agggcttctc cgcgatcacc cgcgtccacg gcgtgcagcc gggagtgtgg
     6901 acacaccccg atggcccgag ctgttccacg atggtcgcga aatactccgg cgcaacggcc
     6961 aggtagtaga gccggttgcc gggagcgccc tgttccgctt caatcgcatc gagccgcacc
     7021 ttcaggcggg cgtagtcgtc cggcacgtcg aaccgcacct gctgataata gagatttgac
     7081 acgaacgggt cgcagcgatc gctgtcgccg tggccggtgc gcgaatgttt cttcagcgcc
     7141 tcgcacatgc tttgtctgaa gtcgttctcg tcgagagggc gccgtgcagc gcccaccacg
     7201 gcgatacgcc gcggcaggta gcccgcgttc cagaggttgt agatggccgg gataagcttg
     7261 cgttcggcca gatcgccggt tgcgccgaaa atgaccatcg tggccatcgc cgcgggttcg
     7321 atgataaacc ccgaacggtc acgttcgggg gggtgcatga aatgcgtggt agcccggccg
     7381 ggcatgccct gctccttttt tgccgatgcg ccttcgacag atgccgtgcc gccgccggaa
     7441 tctacggctg cggcatcgcc atcctcagct caacccgata tcataaatta tgcggaagga
     7501 cgatcctcgt gacgtctcag caattgttcc cattgctgga tacgcatgtc aaaaaaggtc
     7561 agcaattttt cgtgcagctc cgcattggcg gcggccacca tcgactgatg gttcccggta
     7621 gcgctgtcga ggagcagcac gtcgcgcagg ctctttccat aggcatcggt cacaacgcac
     7681 cccgcttcct ccgcgataag cagcgaggcg gcaatgtcat agggggcgat gccgaggatg
     7741 gcgccccgcc cggcgttaat gaaatgatcc tgcacggcct tcgggatgtc gcggtaaaaa
     7801 cggttcgcga tgtccacaca ggcatccagc tggttggtga gaaggcgcgt caggctgtat
     7861 gaggtgctgt tgcaggcgta aaagccccct ttcaggctcg tgagatcgat cagtttggcc
     7921 gctgtcggaa agatgagctc ggcaggacgg gccggcacgg tcattgacca gaccaccgtc
     7981 tcgaggtccg tgttcttgga cagcttgggt ttgcgatact ggccgtcgac atagacgcgc
     8041 gcgcccttgc cgcgttcggc ataaaatgcg cgggagccca tcagctccat gacgagcgcg
     8101 ttgtcgatgt cgcccagccg gggacgctca atcacgcgcg tgctgccgat ggcgatcacg
     8161 caccattcga agccggattt cgccgcccgc gtgccgtcaa tcggatcgat cacaagcaga
     8221 tgctcgggct gcccgcttgt gaacgtcgtg taccccgaat cctcggaata atacgccacg
     8281 gggagcttgg catcgcggag aaacgtgaga agtgctcgtt ccgccagccg gtcgagctga
     8341 aaagtggcgt cgccgctcgc cgcattgcct atgacttccc ggcctttcga cgcatcaatg
     8401 gcaggctgga ctaccacccg aatggcctcg gccagtgcaa tgagaaagtc gcgatcaagc
     8461 aggctgcctt ttgtgcgggc ggccacactc ttcgcgggcg ccggagcttc ctgctgcgct
     8521 ttggtttcaa ccgctttttt gggagccgct tttgcggggg ctggagtctt tttcttcatg
     8581 gtggccttta tcttggtttc ggggccggtc ttcggcttgt tcgcagacgg tttctttttg
     8641 gttttcgcaa ttgctttgga ggtgcctttg ggcgccgggt gttgtttcac agtcgttttt
     8701 gcgggtttgg ccaatttttt cgccgtaccc ggcgccgcct ttttcgcccg gctgatggct
     8761 ttggtcttcg gcttcgcctt ggtttttgcc atgtttgcct acctcctggt gccgttactg
     8821 tcagagaacc gcggcggtct ggcgaagccg ccgccggagc gtttctcctc accacgctgg
     8881 atactcgtgg tcatgccgcg ccgcaagctg tctcctgact cccgtgccac gcacgggatc
     8941 cgccggagac cgctcaggcc aaggcgacac ggggcggagc gatgaagtcc atgaaccggt
     9001 ctttcacctt gccgcgcgcc gcgtctcccg ggtggatcaa aagccggtac cgccacatgg
     9061 tccggcttcc attgccaaag gtcatgtctc ccttcattcg cgcatgcggg cgatagtgct
     9121 tccatgcaaa gcagttggcg gtcattaagc cgtagtcgcg cacatgccat cccgttgggt
     9181 atcgcgggtt ggtctcatgg tccatgatgg cgatgcccgc atgcaccccg tcgaccacgc
     9241 cgctgtaatc gcaccatgga gcgtttttgc cccaggtctc ggcttcgttg atccccccgt
     9301 aggcgttttc gatccgcccc gtgcgcggga catccatcga actggcgacc cgcaccgaaa
     9361 ccagaccgcc ctctttggtg tcgtaaaagg tgatgggccc ttccgtcatc cgaaacgtca
     9421 cagcgagatc gaacaggcgg gttcctcccg gcagggcata gaaccgcatg gcccgagttt
     9481 cttcgaactg tttccgtccg cggttcgtgc accagtcgtt ttttgccacg atctccccga
     9541 aaacggctcc ggattccttc ttgagaaacc ggatgtgccg ctgccggccg tggccgggta
     9601 attcggacca gttatccact ttggctgggc cgcaggcgcc ataggctacc cagatggatt
     9661 tatggtgagg atgatcccgc ttttcgcctt tcacctggct ggataccggc cagctccggg
     9721 tgacccgggc gccgccgggg ccgatcacgg gatatagaaa cgggcgagcc caccgttcgc
     9781 cgtagtggta ggcagtaaat ggcttaccaa agatggaaac gttcaccacc gagtcgcccg
     9841 ggttatcctc gaccgtcacc gtgggtttat gcgaggcgtt cgggacgggt ttggcagtca
     9901 agtgccgggt ttcgccggat ttcaggcctg tgacgagcca gctgagcgtg attccctggc
     9961 cgtgcgggga aacctggctc ggtatggttt ttccggattt tgcgtctttc agcagtaggc
    10021 ccctgttttt gctgtaatct actggcaatt caacgcttac cgggcaaaaa gaccgatcat
    10081 gtggccccgc ttcgacggcg agcctgaaca ccgttggcac tggtcctttc cttccgcttt
    10141 gcgctgttct gccgcaaatc aagctgcgca gtagtctatc atgatatgtc ctgtaagtcc
    10201 agtgtttatg gtgtgttcac ccagacaggc ggagaatcgg agtttcacgt gcttccgtgt
    10261 tggagaaagg cacggcgcac agaggcaggt accaggcgcc gccgtgtcac aacgggctat
    10321 tcttgtctgt cgagagccgt atgaaacagg ttttacagaa ttgacaagac tttttcgaaa
    10381 acctcggagc agcagcctgc gtgcccgttt gtggacgtag atccgctatt tggttttgcc
    10441 agccgcctgg gcgtcttccc aaaagcccgg gtagttgggg ccgtagagca gtgaattttc
    10501 acgcacgacc agttttccgt ccttgacaaa ctgctcgccg cgcacggaaa ggtgggcaat
    10561 catgcgctcg cgccagcggg cggtctcggc cggatttgtc tgggagagat cggccaactc
    10621 gctggggtcg gcgtcgaggt cgaacagctg ctcttcgccc aggtgcgcat ggtaaatgta
    10681 cttccagcgg ccgtcggtaa gggcgttcca gtgattgtcc gggccgtaac agcgcccatg
    10741 ttcgaggtcg agccagggcc gccatccctc cgtttcgccg cgcaccagtt tgagcaggct
    10801 gtcgccgtcg atatcgccgg gcacgccggc tccggcggta tcgaggaaag ttggcatcac
    10861 atcgcgcagt tcgacgggct gccgcaggcg cagccctcta gttgcgcgca ccaggccttc
    10921 cggccagcgg atgatcaggg gaatgcgggc cgacgcttcg tacgcgtagg tcttgcgcca
    10981 gagatgatgg tcgccggtca tatcgccatg gtccgccgtg aatagaatca gcgtgttttc
    11041 aagccagccg cgttcctcga gtatattgag catgcgcccg atctgttcgt caatgaaggt
    11101 gacattccca taatatccaa tgcgcgactt gcggacctgc ccggggccca ggtcgccgtg
    11161 ccagaggttc gggtcgtcgc cgcttggctg cgcgtatttt cccgcccagc cgcccacgac
    11221 cgccttggga atatcgcgct ccgcgtacat cttccagaag cgctcgggag ggtcataggg
    11281 actgtgcggc cgcgcgaacg agaccttgag gaaaaaaggc tcttcgcgtt cgtaattcgc
    11341 cagaaaattc accgcggtgt cgccggtcca ggcggtgggg tggaggtgct caggcagcac
    11401 atagggcctg gcgtcgtatt cgttgaaccc cagtccggtg gcatgccaat ccagcgtggg
    11461 cgcctgcgaa taaaaccacg acacgtagtc gctgcgaaaa tcgatggatt cgacccggct
    11521 cgactcgtcg aggatggtct ggtgaaagcc gtgcaggctg cgctgcggcg accagtgcat
    11581 tttcccaatg cccagcgtgt agtagccggc attccgcatt tcctgaggga gttcgcgggg
    11641 gtatttccag cccactctgc catacgccag catcccgtgg ttccagggcg acaagccaga
    11701 gagcagcccc gcccgcgcgg gcgtgcagct cggcgtcgag gtgtatgcct tcgagaacag
    11761 cgctccttcc cgcgcgaggc gatcgaggtt gggcgtactg atggcggcgt tgccatcggc
    11821 cccgacgcaa tcggcccggt actggtctgc catgatgaag aggatattag gcttgcccgg
    11881 aggcgcttgc gctgtgctcg tatacggcgc ggccgcccag gccgcggcgc cggccgcgct
    11941 gagcttgaag gcttcacgcc ggctgatggg ccgtccagtg tgcatcgtgg taccttcctt
    12001 tccggcggcg tttgcccaac cgcctcggat ctatgatttg cgcatggcta cgttgagaag
    12061 ctgccgggca ttgtcgttga aaaagtcgag aatgtggtcg cgattgattc ccagccgttc
    12121 cattgccgtg cgaatggccc agatctgctc gtagaggcgc agggtaaacg gcggggcgtc
    12181 tccatcggag tcttttgtat gttcgggggt tgcttcgacc acgtaatcgt tgatgatgac
    12241 gctcttcccc agttgcagcg cacggaggcc acccgtggcg aagagaaccc ggtgaggttc
    12301 gatggcggca aagaggcttt cgagtatctg ccattcggta acgcacgccg tggtgaaaaa
    12361 caggttcaac acgtctttga actggtacag cccctgggca gcgtcggtca gcgtctggta
    12421 tttccccgcg gattcgacga caaatttcgc gcgggggtac tgcatgcaca gttctgccac
    12481 ctgccacgcg gtgttttcgt cgctgaggct gatgtccagc accagcagcc ggcccagccg
    12541 atcgagaatc tgcatcgccc attccggcag ggctgccgtc agatcttcgg aggagtccag
    12601 cacaatgccg gcgaaacgct tcgagcccag ggcctcttcc agggcctccg gtgcgatcaa
    12661 atccggcgcg aatcccatca gcccgggcac accctggcgg ctgctggcct gctccacgta
    12721 ctcgttcgtg gcggggtccg cctccgcggc cgcgcagtgc acgtggatcg gccggttgaa
    12781 cagggcctgc gagagggccg gctgcagcga ttctgcggtc atgttcccgg
//
LOCUS       DAOPBQ010000033        12822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4862_length_12822_cov_3.999217, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000033 DAOPBQ010000000
VERSION     DAOPBQ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12822
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4862_length_12822_cov_3.999217"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..802)
                     /locus_tag="PKW60_02160"
     CDS             complement(317..802)
                     /locus_tag="PKW60_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPJ98266.1"
                     /translation="MKKNLGFTLIELMIVVAIIAIIAAIAIPSLLRSRMSANEAGAIG
                     SLRTISAGEVSFQAACYQDADGDGVGDYGTLAQLENPDGVAGVPGYIDEVLAAGSKHG
                     YSFNLVAVAGGAGAPPTYTCNADPQSPQSGRRTFFLDESGVIRFENTGAAATANSTPL
                     G"
     gene            1331..5618
                     /locus_tag="PKW60_02165"
                     /pseudo
     CDS             1331..5618
                     /locus_tag="PKW60_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
     gene            complement(5641..6129)
                     /locus_tag="PKW60_02170"
     CDS             complement(5641..6129)
                     /locus_tag="PKW60_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPJ98267.1"
                     /translation="MRTRAGFTLVELMIVVAIIAIIAAIAIPSLLRARISANEAAAIS
                     SLRTLSSAQISYQAGCYQDANADGIGDFGTLLQLRDPDGVAGVPGFIDEALASGNKQG
                     YNFVMNPVAGGGGNPPTFSCNADPQSPQTGRRTFFVDESGVLRFESNGASATAASPPV
                     GA"
     gene            6600..8036
                     /gene="murC"
                     /locus_tag="PKW60_02175"
     CDS             6600..8036
                     /gene="murC"
                     /locus_tag="PKW60_02175"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPJ98268.1"
                     /translation="MKRIKRKVHFVGVGGIGMSGLAEILLNLGYETSGSDLQPTVITD
                     RLAQCGLRFHEGHDASHVGDAEIVVVSEAVPHDNAEVQAARERGTPVLHRSELLADLM
                     RLKPNAIAVGGTHGKTTTTSMISAMFERANFGATSIVGGILHRSGTNARWGTGEYLVA
                     EADEHDGSFLRLHPTISVVTSIDAEHLEYYGTLDRIKRAFVDFCNVVPFYGYSILCWD
                     DPNARDILGEIDSVCLTYGLERGASLRGANVRWSEGHSHAPMSRRLAELRTSIDVISE
                     DERLGVTGELGALEVKSLGVHNVRNALAACCVGLCLGLKFPHIADGLRMYDGVRRRLQ
                     VCGEERGVLVVEDYAHHPTEIESTLEAARWIRPNRIIVIFQPHLFSRTKFFCEQFAEV
                     LARADRAIVTDIYPSREEPMPGVSSGLIVDAARAQGAQNVQLINDMFSVPGLIAGELQ
                     PDDLVLILGAGNINQLAGPLLQEIARRP"
     gene            8033..8824
                     /locus_tag="PKW60_02180"
     CDS             8033..8824
                     /locus_tag="PKW60_02180"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPJ98269.1"
                     /translation="MRERSPSDIAPKRRRRPHAMLAFLSPMVKFVAFSCLLWGSGYGL
                     AHYLRESPNYRIQHILVQGTMILSAQEIIEASQVTRADNIFFLDPRAIAERIQALPRV
                     KACAVTRIFPDVIAFAIEERLAVATLVVNNRTYEIDAAGAILDEVAPDAPYCGPLLTL
                     HPPVSTVTPGEPVGRPELVEGLAVWNAYAKTRLAAVAPAHELAVFDSNDIRMYCESMP
                     FEIRWGRGDYDAQARRLDILWQELNGRLPCTAYLDLRFGRDLACK"
     gene            8921..10333
                     /gene="ftsZ"
                     /locus_tag="PKW60_02185"
     CDS             8921..10333
                     /gene="ftsZ"
                     /locus_tag="PKW60_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPJ98270.1"
                     /translation="MVIGMSEEYQSFEDRAVIKVCGIGGGGGNAVGRMIEAGLKDVEF
                     ITINTDAQALRNSPAGTRLQIGSEVTGGLGSGAKPEIGQKSALEDRDRIAEVLRGADM
                     IFLTAGLGGGTGTGASPIIAEVARETGALTVAIVTLPFNFEGPLRHENALKGLGGLEK
                     YVDTLIVVPNDRVAALCHNNISFLNAFRLADEVLHNGVRAISELITVPGLINLDFADV
                     RTIMQARGRALMGIGTAEGENRAVCAAEKAILCPLLEQSNIEGATGVIVNITGGCDIG
                     MREVQQAVSTVQKSAHPGANIIFGAVVVDEERPELQVTVIAAGFTPEAAGGREPFQLY
                     PEPACPEVPVKESVPALARSLDMPEQSGEAPVLPESEEAGPVPDSGIQSEAPAPAPCP
                     PEEVPAERHVSTRQDPFHEPPAGAAAGGAPPEPGLQYMFPEQANDNETESSLCPAGRP
                     QRDEPEDLDIPAFMRRRKKP"
     gene            10447..12318
                     /locus_tag="PKW60_02190"
     CDS             10447..12318
                     /locus_tag="PKW60_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPJ98271.1"
                     /translation="MPVRAARGLVMAAVLLAATASASIVEFQWHLQRTLRDEGSTAVD
                     CQRAIAALLGQAASFSGSILVNGEAVDGKAVASQTFSDHMDSSVVRAQSITVPASSGW
                     PVTLSVPRPADGHVITVYQRIGEDVYIRVPPASGAYPAAIGFLLRFPGEYIVAEEEES
                     PESPPAGAFPMGDFFDFSPVSAAPEAKAAWDFYPVAPDVIAGPVPLILIHGMVSDRWN
                     DFAHWAEHSDEAAAFREHFQLWNFSHPGSGVTAPIGFSTAYPSFSESVVAYLARFIQK
                     AEANGVESGGKRYVFPSGPYALLTHSAGGLKARAFLVNYPEHAQRVFAVVTLACPHMG
                     TPWGTPEWVRHTLSGIGFTREFIGEKIFQGLLAELTLNGYFNMKRQGDLDVGWANYDA
                     AGGFGIPTRTFRAWFWPWYLREITVSPRDANLTHARELAGIDDQTFEPAELFGTYCGG
                     LDLITPAERGGMHLDKFFIYASYVDVVEDVTRLLNALAPDEHENRYTMNSALRLVQQL
                     MSTVESEGAAYPIGTYRVGDGFVPLQSQLFLDGTETERIYETQIVHGWEIAAFPTKLR
                     EEIIFEHTLALPERLRILKGWSHLDTITGRYCLESGRSELFPRVVEDLLSVMPPPED"
     gene            12426..>12822
                     /locus_tag="PKW60_02195"
     CDS             12426..>12822
                     /locus_tag="PKW60_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98272.1"
                     /translation="MRWSLLAAGILLAGVLCAARVAEAQESDPSATEEVVVTPVAEGA
                     HPEQLTLAVMIREGGGILYIIIGLSVITFIMLIYFFFTITPAREVPPNLLKRALSQIR
                     AGDLRGAYQMCDGRDELLANVIKSGLKMAG"
BASE COUNT         2498 a         3914 c         3841 g         2569 t
ORIGIN      
        1 tgcagccata tcagcgagcc cgggtgcgcg gtccgggacg ctgtcgaaac cggcgctctc
       61 gctgccgtgc gctacacatc ctactgccgg atccgcgaca gcctcgattc gaagagtccg
      121 tggggctgaa ccttcagcgc cgcgtcaccc cggaccgttt ctcaaagctg ttgaccattt
      181 ttcctcaggc ggcatcgaat atccccatgc atcggtgctt gcgacccaac aaagagaaaa
      241 agagccctga atatagcgaa acggcggcct ccgaagagag ccgccgtccg ctgtgctgtc
      301 tgtgctgttg tcccgattag ccgagaggag tcgagttcgc ggtagctgca gcgccggtat
      361 tctcgaaccg gatgaccccc gattcatcca ggaagaaggt ccggcgaccc gactgcgggc
      421 tctgcggatc cgcattgcag gtgtacgtag gaggcgcacc cgcaccaccg gcgacagcga
      481 ccagattgaa gctatagccg tgcttcgagc cagcggccag cacttcatcg atgtagcccg
      541 gaacccccgc cacaccatcc ggattttcaa gctgcgccaa cgtgccgtaa tcccccacac
      601 cgtcgccgtc ggcatcctga tagcaggccg cctggaagct cacttcgccc gcggaaatcg
      661 tgcgaagcga accgattgcg cccgcttcgt tggcgctcat gcgcgaacgc agcaggctcg
      721 ggatagcaat cgccgcaata atggcgataa tcgctaccac gatcatcagc tcaatcaacg
      781 tgaaacccag attcttcttc atttccatgc cctccgttga gcgtcccatc accaggcctg
      841 tttccaggcc gccattcctt caagtgccga caatcccttt ggcaataggc gtgccaactt
      901 tgccggagat tcacaacaca ttgcgccaca gacagatagg aaattggcct ccagattttt
      961 gcccgtttac cggctggtta cagctacgtt gtacggcacg gaatgacaat ttttgtcaat
     1021 ttaaccccgc aaacaccgcg tgcagacccg cgaaaagagc gtctctggct ggaagactcc
     1081 cagacctgcc ccgttaaggc ggacgtgctg caaaaccggc tcttttcaaa ggaattatcc
     1141 gattgctgac aggtttcaat ctggcgcgaa cttgcctcag atttacctgt cttgtaagat
     1201 gtattctccc ggagattgcg ccccggaatg gcgcagggca aggaagcgct taaacgtccg
     1261 gcgggacagc tttctctctg gtgtcgacaa taataatacc aaactgttac agtctcaggg
     1321 gtctgcggta atgaatccac agtctttggg aatttgcgtg ggagcgtcca ccatcggcac
     1381 ggtgcgcttg cggcggggtc cgtggggcgc ggaggtagtt tccgcggaga cccgcgccca
     1441 tgatggcaac ccgcggggtg ttcttcagga gattttctcg caaattggcg acttgcacag
     1501 tctgcgcgtc gctgccacgg gccgcaaatt ccggagattt cttgagctgt caaccatatc
     1561 tgagcccgaa gcggtcgaga aggcagtggc tcataccttc ccgggcggcc atccctaccg
     1621 gctcgtcatc agcacgggag gggagacctt catggtgtac catctcgatg acgacagccg
     1681 cattcaggga atctgcacgg gcaacaaatg cgcatccggc acgggcgagt tttttttgca
     1741 gcagctcggg cgcatggaca tctccctcga cgaagtggag cgcatgcctc agccggatac
     1801 ctggcataag gtctccgggc gctgttcggt attctgcaag agcgactgca cccacgccct
     1861 gaacaaaggc atccccaagg cgcaggtcgt ggccgggctg tcccggatgc tggccggaaa
     1921 aatcgtcgaa ctgctcaaga agctccccaa gacgtccgcg ctgcttatcg gcgggtgcag
     1981 ccggaacgcc tccatggtgc attacctccg ggaacaaatt gacgacctgt gcatcccgga
     2041 atccgccacc tggtttgagg ccctgggcgc ggccgtgtgg gcgctggacc atgacgtcaa
     2101 gcccatcact gatcccgggg ccgtcatgac cttccgcacg tccacgctgt catttctcaa
     2161 accgctgaat gagtaccggg accgggtgca gttccgccat caccccccgg ggatgtgccg
     2221 aagccggaga ccggaccatc gtaggcctcg atgtcggctc caccaccaca aagggtgtgg
     2281 tgatgcgctg cagcgacaaa gccattctgg ctgccgagta tctccgcacc aacggcgatc
     2341 ccgtcggcgc gtcgcgcagg gtctatgcaa gcctcgcgga tcaagtccag gcgcccatcg
     2401 aaattgttgg attgggtgta accggatccg ggcgccagat cgcgggtctc cacgcgctga
     2461 ccgagggcgt tatcaatgag atcatcgccc acgctaccgg agcgctccat tttgatcccg
     2521 acgtcgacac catcttcgaa atcggcggcc aggacgccaa atacacctac attaccaacg
     2581 gcgtcgcgtg cgattacgcg atgaacgaag cgtgcagcgc aggcaccggc tcctttctcg
     2641 aggaatccgc caaggaaagc ctcggtatct ctgtgaccga gatcagccga acggcgttcc
     2701 tgggtgaaac gcccccaaat ttcaatgacc agtgtgccgc gttcatcggt tccgatatca
     2761 aaggcgctgc ccaggaaggc attcctgtgc cggatatcgt cgcggggctg gtctattcga
     2821 tctgcatgaa ctacgccaac cgcgtgaagg gaaaccgccc cgtcgggcgg aaggtcttta
     2881 tgcagggcgg ggtctgctac aacgaggcca tccccgtcgc catggccgcg ctcaccggga
     2941 aaacgatcat cgtgccgccc gagccgggcc tgatgggcgc ctttggcgtg gcgctcgagg
     3001 tcgaacggcg catcgaagac ggcctgctgc ggcctcaacc ttttgatctc aacgacctgg
     3061 ccgcgcggga cgtcacgtac ctgagcccgt tcatttgtaa cggcggcagc gatgggtgcg
     3121 acctcgcctg cgaaatcgcc cgcatggaaa tcgacggcct ggttttccct ttcggcggga
     3181 tctgcaaccg ctatgacaac caattgcgca accggcgtat cgacaccgcc ggtctgaatc
     3241 tggttgtcca gcgagagcga aggctgcacc tggattcggc ggccgaaaat gaatcagcat
     3301 cccaacccac cgtcggcttg aaccgctcgt tcatggtcca ctcgtatctt ccgttttacc
     3361 ggaaattttt caacagcctc ggattccgcg tcgtgctgcc ggaagaagcg gacccccgag
     3421 gcgcggacca gcagggggcg gcgttctgtt tccccgccga gcttgctcat ggctacgcgg
     3481 cgtcgctgct cgccttgaaa cccgattatg tgtttttgcc ccacatcggt ggcgtgcaaa
     3541 gcaccgaagc ctgcagtcca gcctgcacct gtgtctttgt gcagggcgaa ccctattatc
     3601 tgaaagccgc ttttcccgag ctggacgccc cacacgtgct tcagccttac ctggactttg
     3661 ctcaggggct tgagtccgtg caggaatccc tcaaaggcct ggccgcctcg ctcaaacgag
     3721 aaccagaggc gattgcggat gccgcaaaag ccgcctggga ggcgcagacg gcgttttctg
     3781 acgagctgcg ccggatgggc cgtgaacagc tgcgccgctt ggaggaagac ccatcgctca
     3841 ccggtgtggt gctctttggg cgggcctaca acagcttcac accggaggcc aataaaggaa
     3901 tcccggccaa gtttgcgtcg cggggagtgt gcatcatccc gtatgacatg ctgccgtgcg
     3961 acgatcgccc gctgccggac gaacacaaca tgtactgggg gaccgggcag gcactcttgc
     4021 gcgccgcgcg gttcgtcaag gatcatcctc aacttttcgg cgcctatatc acgaactttt
     4081 cctgcgggcc tgactcattt ctcatcacct acttccgtga catcatggga cggaaaccgt
     4141 cgttgaccct cgaactggac aaccataccg cggatgccgg catcgagacc cgtgtagaag
     4201 ccttcctgga tatcgtccag catttccgcc acatcggaca gccggcccgg gcgaaggcct
     4261 cgcttttcgg gaggccgctc cagcctgctg ccgtggagta tcgcgggggc gccgcaggta
     4321 ttcgccgctc atcgggcgaa tgggttcccc tgagcgaccc gagggtcaac gtgatcattc
     4381 ccgccatggg ccgctatggc acggcgcttg ttgccagcgc cttcggccgc cggggcgttc
     4441 gcgccacccc gctgccttcc gccgacgacg aaatcctcaa attggggcgc gcacattcca
     4501 cctgcaagga atgcctgcct cttcagacga cgatcgggtc gatggtgcaa tacttggaac
     4561 ggcgcccgcc ggacgagatc accgcatatt tcatgctcag caccgacggg ccctgccgtt
     4621 tcggtcagta ccatgtcttt tcaaaggggc tgatagagaa actcgggatt gccgatgtcg
     4681 ccgtgctctc cctgaccacg gccaacggat atgcgggcct cggcgaccgg tttgcgctga
     4741 ccgcgtggct gggcgtcatt accggcgacc tgttcgatga tatgtattcg atgatccttg
     4801 ccgccgcggt cgatcctgcc gaaggattgc gcatcctgga cgaagaacat gcggcgctcg
     4861 ttgcggcgct gcgtaaagga tggcccgcag ccgcacgcca gctgtcgcgt tcgggaaaac
     4921 ggcttggcgc catccctctg aagaaaccca gccgtgaggt tcccgtcgtc tccctgatcg
     4981 gcgaaatcta tgtgcgccgc gatcccatct cgctccaggg gctggtggaa catctcgccc
     5041 gggaagggat cgccgtgcgc accgcgcaga ccagcgaatg gatcaaatac ctggactggc
     5101 tggctcaagt gggtatcgac ggcgaacggt cgctggcgtt ctggctgcgc tactgggtga
     5161 aacgctacct cgaccgggga gtgcgcaaac ggctcgcgct ctccaacctg ttctattgcg
     5221 acggattgga tgtggagccc attattcaag ccggttcccg gtttatctcg ccccgtttga
     5281 ccggcgaggc catcttgacg gttggcaatg ccctgcatga aattctgcac ccttcatgcg
     5341 gcattatcgc catcggcccg tttggctgca tgccatcgcg cgttgccgaa tcaatcctga
     5401 ccgaaaagct gaccaccacg gaaaaacgcg ccgttgccca tggaaacgga cactcctcct
     5461 gggatgcact gctggagact caccgcaaat tgccttttct cgccattgaa accgacggga
     5521 acgcttttcc gcagatcatc gatgcgcggc tcgaggcatt tctccttcag gcccgccgcc
     5581 tccacggacg cctcaattca gccagcgccg gtaattgata cgccctgtgc gaaggcgctc
     5641 ctacgctccc accggcgggg acgcggccgt cgccgaggcg ccattgcttt caaagcgcag
     5701 aacgccggac tcgtccacga aaaaggttcg ccgcccggtc tgtgggctct ggggatccgc
     5761 gttgcaggaa aacgtcggag ggttcccgcc gccccccgca accggattca tcacaaaatt
     5821 gtagccttgc ttattgccgc tggccagtgc ctcatcaatg aatccaggca cgcccgctac
     5881 tccgtcagga tcccggagtt gcagcagcgt tccaaaatct ccaatgccat cggcattcgc
     5941 gtcttggtag cagcccgcct gataactgat ctgcgcggag gacagtgtac gcagagaact
     6001 gatggcggct gcctcgtttg cggaaatccg agcgcgcagc aggctcggaa tggctatcgc
     6061 tgcgatgatc gctatgatgg ccacgacaat catcaattcg acgagcgtga atccagctct
     6121 cgtcctcatg cctgaaacct ccaagtttaa cgcctgcgat ctcctctgtc ggctattcaa
     6181 tgaaacgcaa atgcagtgcc aatttcatgg tcaagcgcaa tatctttcgc cccaaacctt
     6241 tgaaggtttc atcccgcatg tttcgccgcg ggcgtctccc cggaaacgat gaattcccgg
     6301 catattgacg aactgtcgga tgggcagctg ctgaattttc ggatgcgatc cgggcctgtg
     6361 gcgatgtctg agagacacca gttgtgcaga ttttgaccgg cctaccacaa ggtctagggg
     6421 tatcagggaa tcgcacaagg ttgaggcttg aagcacggcg ttcgtgcgta aagatttgcc
     6481 gtgcaggagt ttatggggcg atattcttca ccggagtccg cacaaagagt actttccctt
     6541 gtggtaatcg ttgtgctata cttgaaaata ccacaatata ttgggtgggt gaggtcgatt
     6601 tgaagcgaat caaacgaaaa gtgcattttg tcggcgtcgg tgggatcggc atgagcggtc
     6661 tggctgaaat cttgttgaac ctgggctacg aaacctcggg ttcggatttg cagcctacgg
     6721 tcattacgga ccgcctggct caatgtggcc tccgctttca cgagggccac gatgcgtcgc
     6781 atgtgggcga cgccgagatt gtggtcgtgt cagaggccgt gcctcatgac aatgccgaag
     6841 tgcaggccgc gcgcgagcgt ggcacgccag tgctccatcg cagcgagctt ctggccgatt
     6901 tgatgcgcct taaaccaaac gccattgccg tgggcggcac ccatggcaag acgacgacca
     6961 catcgatgat tagcgccatg tttgagcggg ccaacttcgg cgcgaccagc attgtgggcg
     7021 gcatcctgca ccgcagcggc accaacgccc ggtggggcac gggagagtat ctcgtcgcgg
     7081 aagcggatga gcacgatggc tcgtttcttc gtctgcatcc cacgatcagt gttgtgacaa
     7141 gcatcgacgc ggaacatctc gaatattacg gcacgctgga tcgcattaag cgggctttcg
     7201 tggatttctg caatgtggtc ccgttttacg ggtattcgat cctgtgctgg gacgatccca
     7261 atgcccgcga catcctgggt gagatcgaca gcgtatgcct cacgtatggc ctggagcggg
     7321 gggcctcttt gcgcggcgcc aatgtgcgct ggagtgaagg gcattcccac gcacccatgt
     7381 cgcgccggct tgcggagctg cgcacctcca tcgatgtcat cagcgaagac gagcggctcg
     7441 gggtgactgg agagctcggc gccctcgagg tgaagtcgct cggcgtccac aatgtgcgca
     7501 atgccctcgc cgcctgctgt gtgggacttt gcctcggcct gaaattcccg catatcgccg
     7561 acggtctgcg catgtacgac ggggtccggc ggcgcctgca ggtctgtggg gaagagcgcg
     7621 gtgttctggt cgtggaagat tacgcacacc atcccaccga aatcgagagc accctcgaag
     7681 cggcccgctg gatacggccg aaccggatca tcgtgatttt ccagccgcac ctgttcagcc
     7741 gcaccaaatt cttctgcgaa caatttgcgg aggtgctggc cagggcggat cgcgcgatcg
     7801 ttaccgacat ttatccgtcc cgcgaagagc cgatgcccgg cgtgagttcg gggctgattg
     7861 tagacgccgc ccgggcacag ggcgcacaaa acgtccagct gatcaatgac atgttttcgg
     7921 tacccggcct gattgcaggg gaactgcagc cggacgacct ggtgttgatt ctgggcgcgg
     7981 gcaatatcaa tcagctcgcc ggaccgctgc ttcaggagat agcgagacgg ccatgagaga
     8041 gcgttctccc agcgacatcg ctccaaaacg ccgaaggcgc ccccacgcca tgctggcgtt
     8101 tctcagtccg atggtgaagt ttgtggcctt ttcatgcctt ctatggggta gcggctacgg
     8161 gttggcgcat tatctccgcg aatcccccaa ttaccggatt cagcacatcc tggttcaggg
     8221 cacgatgatc ctgagcgcac aagagatcat cgaggcttcc caggtcaccc gggctgacaa
     8281 catcttcttc ctggatcccc gggcgatcgc cgagcgcata caggcactgc cccgggtgaa
     8341 ggcatgcgcc gtaacccgga tctttccgga tgttattgcg ttcgcgattg aagaacgcct
     8401 ggctgttgcc acgctcgtcg tcaacaaccg cacctatgag attgatgcag cgggcgcgat
     8461 cctggacgag gtggcgcctg acgcccccta ttgcgggccg ttgctgacgc tccatccgcc
     8521 tgtcagcacg gtcacgccgg gcgaaccggt cggccgcccg gaactcgtcg aggggctggc
     8581 agtgtggaac gcctatgcca aaacccgcct ggctgccgtc gcgcccgccc acgaactggc
     8641 ggtatttgac agcaacgaca tccgcatgta ttgcgagagc atgcccttcg agatccggtg
     8701 ggggcgcgga gattatgacg ctcaggcgcg ccggctggat atcctctggc aggaactgaa
     8761 cgggcgtcta ccgtgcacag cgtatctgga tcttcgtttt gggcgggatc tggcctgcaa
     8821 atgaatggcc gtgtttgcat aacgattttg aggtcgccgc gtagctcgcc gttgccggtc
     8881 accggctaca gccgcacgca accgtgaggg agacaggacc atggtgatcg ggatgtcaga
     8941 agaataccag agctttgaag atcgtgccgt catcaaggtg tgcggtatcg gcgggggggg
     9001 cggcaatgcc gtaggccgga tgatcgaagc cgggctcaaa gacgtcgagt ttatcacgat
     9061 caacacggac gcgcaggccc tccggaactc tcctgccggc acccggctcc agatcggttc
     9121 cgaggtgacc ggagggctcg gttcaggcgc caaacccgag atcggccaga aatcggctct
     9181 cgaagaccgg gaccggatcg cggaagtcct gcgcggcgca gatatgattt ttctgaccgc
     9241 gggcctcggt ggcggtacgg gcactggcgc ctcaccgatc atcgccgaag tggcccggga
     9301 aaccggcgcg ttgaccgtgg ccatcgtcac gttgcccttc aatttcgaag gacccctgcg
     9361 ccacgaaaat gcgctgaaag ggctcggagg actcgagaaa tatgtcgaca ccctgatcgt
     9421 tgtgcccaac gatcgggttg ctgctctctg ccataacaac atctctttcc tgaatgcgtt
     9481 tcgtctggca gatgaggtgc tgcataacgg agtgcgcgcc atctccgagc tcatcaccgt
     9541 gccgggattg atcaaccttg attttgccga tgtacgcaca atcatgcagg cgcgcggccg
     9601 ggccctgatg ggcatcggga cggctgaggg agagaaccgg gccgtctgtg cggcggagaa
     9661 agccatcctc tgcccgctcc tcgaacaatc gaatattgag ggcgccaccg gcgtcattgt
     9721 aaatattacc gggggatgcg acatcggaat gcgcgaagtg cagcaggctg tatccacggt
     9781 acaaaaatcg gcccatcccg gtgccaatat catttttggc gcagtggtcg tcgatgaaga
     9841 gcgcccggaa ctccaagtta cggtcatcgc ggccggattc actcccgagg cagcgggcgg
     9901 gcgcgaacca ttccaattgt acccggagcc tgcctgcccg gaagtcccgg tgaaggagag
     9961 cgtgccagcg cttgcgagga gcctggacat gcccgaacaa tccggggaag cgccggtttt
    10021 gccggaatcg gaagaagccg ggccggtgcc ggattccgga atccagtctg aagcgcctgc
    10081 cccggctccg tgcccgccgg aagaagtccc cgcggagagg cacgtatcca cccggcagga
    10141 cccgtttcac gagccgccgg ctggtgctgc cgcggggggc gcgcctccgg agcccgggct
    10201 tcaatacatg tttccggaac aggcaaatga caacgagact gagtcttctc tctgcccggc
    10261 tgggcgtccc caaagggacg agcctgaaga cttggatatc ccggcgttta tgcgcaggag
    10321 gaagaaaccc tgaccgaagg ccgcactctt ttgcggcatg aatctgatcg tgtggagtgg
    10381 accagtacca tgcaaactga atctctcaga ccagctcttt cggcgtcccg gcgacaggcc
    10441 ggcggaatgc ctgttcgagc cgcccgaggg ctcgtgatgg cggccgtgct cctggcggcc
    10501 accgccagcg ccagcatcgt cgagtttcag tggcatttgc agcgcacgct ccgggatgaa
    10561 ggttccaccg cagtggattg ccaacgcgcc atcgcggcac ttctcgggca agccgccagc
    10621 ttttcgggca gcatcctcgt taatggcgaa gctgtggacg ggaaagcggt ggccagccag
    10681 actttttcag atcacatgga ctcgtccgtg gtccgggcgc agtcgataac cgtgcccgca
    10741 tccagcgggt ggcccgtgac gctttccgtc ccgcgcccgg cggatggcca tgtcatcacc
    10801 gtctaccagc ggattggcga agacgtctac atccgtgtgc cgcccgcttc gggagcgtat
    10861 cccgccgcga tcgggttcct tctgcgtttt cccggcgaat atatcgtggc tgaggaggaa
    10921 gagagcccgg aatcgccgcc agccggagcc ttccccatgg gcgacttctt tgatttcagc
    10981 cccgtctcgg cggcgcccga ggccaaggcc gcgtgggact tctaccccgt ggcaccggat
    11041 gtgatcgcgg ggccggtgcc gctgattctc atccacggca tggtgagcga ccgctggaat
    11101 gatttcgcgc actgggccga gcacagcgac gaggccgccg cattccggga acatttccaa
    11161 ctctggaatt tcagtcatcc cggctcggga gtgacggccc caatcgggtt cagcacggcc
    11221 tatccatcgt tttcagagag cgtcgtcgcc tatctggccc gcttcattca gaaggcagag
    11281 gcaaacggag tcgagagtgg cgggaaacgc tatgtttttc catcggggcc ttacgcgctt
    11341 ctgactcaca gtgccggagg actcaaggca cgcgcgtttc tggtgaatta tcccgaacac
    11401 gcgcagaggg tgtttgcggt cgtgaccctt gcttgccccc acatgggcac gccctgggga
    11461 acgcccgaat gggtccggca cacgctgagc ggcattggtt tcacccgcga gttcatcggg
    11521 gagaagatct tccaggggct gctcgccgag ctcacgctta acggctattt caatatgaag
    11581 cgccagggcg atctggacgt gggctgggcc aactatgatg cagccggcgg gtttggcatc
    11641 cccacccgca cgttccgggc ctggttctgg ccctggtacc tgcgcgaaat caccgtgtcg
    11701 ccccgcgacg ccaacctcac ccatgcccgg gaactggcgg gcattgacga ccagacattc
    11761 gaacctgcgg aactgtttgg cacctattgc ggtggtttgg acctgattac gcccgccgag
    11821 cgaggcggca tgcatttgga caaattcttc atctacgcca gctatgttga tgtggttgag
    11881 gatgttacgc gccttctcaa cgccttggcg cccgatgagc acgaaaatcg gtatacaatg
    11941 aacagcgccc ttcgactggt tcagcagctc atgagcacgg tagaatcgga gggcgcagca
    12001 tatcccattg gcacatatag ggttggcgat ggttttgttc cgctgcagag ccagcttttt
    12061 ctggatggga ccgagaccga gcggatctat gagacgcaaa ttgtccatgg ctgggagata
    12121 gcggcatttc cgacgaagct gcgggaagaa attatctttg agcacacgct ggccttgccc
    12181 gaacggcttc gtatccttaa aggctggagc catctcgata ccattacggg gcggtactgt
    12241 ctggaatccg gccggagcga actgttcccg cgcgttgtgg aagatttgct cagcgtaatg
    12301 cccccgcccg aggactgagt ttcctgcctt ttgcaaccag aatccccctc aaggcataat
    12361 aatctctatg aattcggagg ggcatgcctt cggatacaca actcgggaaa ggagcagtgc
    12421 tcacgatgag gtggagtttg ctggcggcag gaattctctt ggcaggcgtc ttgtgcgcgg
    12481 cacgggttgc agaagcacag gaatccgacc cgtctgcgac agaagaggtg gttgtaaccc
    12541 ccgtggcgga gggcgctcac ccagagcagc ttacgctcgc tgtgatgatc cgcgaaggcg
    12601 gcgggatcct gtacatcatc atcggcttga gcgtcatcac gttcattatg ctgatctatt
    12661 tcttcttcac cattaccccc gcgcgcgaag tcccgcccaa tctattgaaa cgcgcgctca
    12721 gccagatccg cgccggagac ctgcggggcg cctatcaaat gtgtgacggc cgcgacgaat
    12781 tgctcgccaa cgttatcaag tcgggattga agatggcggg ac
//
LOCUS       DAOPBQ010000034        12610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_4963_length_12610_cov_3.908005, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000034 DAOPBQ010000000
VERSION     DAOPBQ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12610
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4963_length_12610_cov_3.908005"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..1040)
                     /locus_tag="PKW60_02200"
     CDS             complement(330..1040)
                     /locus_tag="PKW60_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPJ98273.1"
                     /translation="MMKKVLSSVAVAVLVSFSAAALTVADVNVQPAHPGEWELLDIYE
                     AIFGVSTGFTTSDALYQAVGVDPDDYNGPDVFHFFGGQNVALEARYATYNHSLYYYDP
                     VLPGAVENENFLFTVTGGYVNPGAGGSPAAQLIASIEPYGFSLHVGQTGATLYSEYGR
                     NEAERVQMLMLAAPQGYEPGTYLLAWEDRLAGDPRADWDYQDLVILVRPIVPEPTSIA
                     LMGLGIAALSLRGLRKLV"
     gene            1977..2684
                     /locus_tag="PKW60_02205"
     CDS             1977..2684
                     /locus_tag="PKW60_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98274.1"
                     /translation="MKQICAVSDLHLFCKRSEAHRHTAALDRAIAEAEVCVLNGDIFD
                     FRWSVFDSVEETVDEALAWLDRIIARHPHCDFHYIFGNHDSVKVFIDALDAYAARTER
                     LTCHHDLFRLNAAVFLHGDVADRKMTPAEFEAARRVWHYDRQRGRIVNRAYDLAVWLG
                     AHRTIPRLMFPRHVVAGRLVHYLEHVGHGPDSGVTDVYYGHTHVAVRGFQYNGLNFHN
                     SGAPLPKLGFTILRATA"
     gene            complement(2707..4146)
                     /locus_tag="PKW60_02210"
     CDS             complement(2707..4146)
                     /locus_tag="PKW60_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPJ98275.1"
                     /translation="MRQDDRRDIDLDGAPDVSFPEVRVSVSRDRMAVLLDASSIPHEL
                     DAFVAWVLGQLGQLQIRPDLLDERIGGRIRAAVSDDAWRGLILAEGDRPGEPVDGYIE
                     WAGDFFKSGFVLDEETGALNYRKPAAERNVQAGQLLATVHPPQPGADGVDVYGNRVPG
                     KKGRSVHLRAGRNVRQEDTAYFATRDGRIQHDMRAVSVDEVLVIRGNVGLKSGDINHL
                     GTVLIEGDIEPNSVVRATGDIEVSGIVEQADVEAGGNLVIRGGIMGRGKYKILVEGRV
                     QARYIIDSKVEAGHDIIVEREIVQSFVCSMGSICVPRGRIVGGEAVARAGFIVGQVGS
                     EALVPTVLTAGRDFRLDSKLRTLRREINELFAQQGQLEMKLKFLATPRRLPMSRPDSS
                     QKDDPAQLQQEMDEVARRIQILTAEAEDLKLHSKNNTRARLEILKVVYPESCLGLAEE
                     ELHVREQFPGPLHAHLANGKVHLRKGSES"
     gene            5306..7927
                     /gene="mgtA"
                     /locus_tag="PKW60_02215"
     CDS             5306..7927
                     /gene="mgtA"
                     /locus_tag="PKW60_02215"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HPJ98276.1"
                     /translation="MKKIRFANSSNGNTVASCVQRAPTGHERRLVQLCTVPVREAVAT
                     LESSLRGLSGEEAEQRLDAFGPNELTHLQPLGFWGDLLRHFKNPLVLQLLIIALVSAG
                     IGEHAPAIIVGAMLLLSVGLSLILDRRSSQAVENLGKRVQSRTFVLRDGAEREVRNSE
                     IVPGDIVLLQAGSIIPADLRLITAKDFFVSESALTGESMPVEKTTALPDDQNIAALEL
                     PNACFFGTSVNSGAARGLVVNTGACTLFGAIAERLAGKPEETDFDRGVRSFTWLMIRF
                     MVVMVAVVFFVVGMSKGDWVQALLFGLSVAVGLTPEMLPMIVTVNLAKGALTMAKRKV
                     IVKRLPSIQNFGAIDILCTDKTGTLTQDRVVLERHVDTLGRPSEEVLDYAYLNSYFQT
                     GLRNLIDRAVLAYSDLDTRQCRLVDELPFDFQRRRMSVVVSYEDDHVLICKGAVEEIY
                     ACCSRYQFEEQVYPLVDMIRGVLFEEVEALNRDGFRVLGIAYREFPSDKTVFSIEDES
                     ELVLLGYIAFFDPPRTSCAEALKRLAGAGVQVKVLTGDNGLVTEKVCRDVGLAVEQIV
                     TGTELAHLSPEQFSSVVLDANVFVKLSPAQKEEIVEALRRDGHVVGFIGDGINDAPAL
                     KAADVGISVDSAVDVAKEAADIVLLEKSLLVLEEGIMEGRRVFANIVKYIRMGASSNF
                     GNMFSVVGASYLLPFLPMQPVQILINNLLYDFSQTGIPTDTVDDELIAKPLKWNIGNI
                     KRFMVFIGPVSSLFDYATFALMWFVFQWGAVFDSRLFQTGWFVESLLTQTLIVHIIRT
                     KRVPFFGSRASLHLTVTTLIIMAIGIWLPYSPFASDLGMMPLPAIYWAWIALFLTAYA
                     VLTHGVKTWFFKRYGGD"
     gene            7930..8715
                     /locus_tag="PKW60_02220"
     CDS             7930..8715
                     /locus_tag="PKW60_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPJ98277.1"
                     /translation="MDTILDALQEGRLFELPDNDKTDALQFLAHIIEAFPEVPAGTDV
                     VGNVLKKEEAANTALGKGWACPHAHVPFDEDLMCVIGWSPAGIDYGATDGKPVTIAIM
                     YLVPENQRRHYLREISLLAKALQTYPGLDKLQEVKDLDDIRNYLLDLIDSSKESAGLD
                     TRARMIRLQAKPAPELPVLQDLSNLLVESVTLVAGPSLKPVVLTQNPDLLKWLESADD
                     LAGKIEVDGSYQNGGWRLVRRGAVAYQGNRLVFDCIAVKLVAS"
     gene            8942..9394
                     /gene="ndk"
                     /locus_tag="PKW60_02225"
     CDS             8942..9394
                     /gene="ndk"
                     /locus_tag="PKW60_02225"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPJ98278.1"
                     /translation="MIERTFVMVKPDGVGRRLIGEVIGRYEAKGLKLVGLKMQILSRA
                     QAEAHYAEHRDRPFFAGLIAFITSGPTIQMVWEGEDAIAAVRKVNGATDCRDADIGTI
                     RGDFGLTKQKNIVHASDARETAEREIALYFQPSELWDYRQPGEAWLQE"
     gene            9668..10138
                     /locus_tag="PKW60_02230"
     CDS             9668..10138
                     /locus_tag="PKW60_02230"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HPJ98279.1"
                     /translation="MADEDKKIFIDEDWKAQVAREKEEARAKSAQQSEPASETDEAGS
                     PQGDQPGADTIEPNFSGLVDSLAMQGLMALGLIAPQDAKEVYVDIASARFVIDTLMML
                     REKTNGNLTPDETGHLSSALGELQRFYVARAQQVQEQAMKQAGVDMNNLRAPRQ"
     gene            10280..11596
                     /locus_tag="PKW60_02235"
     CDS             10280..11596
                     /locus_tag="PKW60_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPJ98280.1"
                     /translation="MRLSCANLCKRNARLHGRALLFLAAAIGGCLASPEPLSPDEALA
                     RAHIQITRLLQEELTRYPEAIDIEAPEAVDNPAAVTLWYPAHSIIAPALGNPEIRRAF
                     DAAHPGIELKPQFIGDWHVAIQKLTVSIAAGGLPDIAIVERSWAARLAQAGRLMPLDG
                     FLDVEMLDDFHPNARAAYTLQGRIWALPADGFCSVLFFNRAAVADPPKTWEELPVQIS
                     ETKAVIGHLPYIEMLWSAGGRVCEHNQSGLTAPSAMRALHFLLSLRDTGQSDAQMLAD
                     PAWGFAAFMKNSAPMTAASSEWLVRTRDAAFPAGMAALPGAEGPISRVGDMAIVVFAK
                     HAAAKHDAIVAALDWLTGPAVQGAAAAARGSMPVRQSVAETVDIPPGLRAAYTAAQAP
                     PFAGVWSAAEYELSSNLARVWQWKHESPESEASRHGGTWETSPSGN"
     gene            11743..>12610
                     /locus_tag="PKW60_02240"
     CDS             11743..>12610
                     /locus_tag="PKW60_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPJ98281.1"
                     /translation="MNILYRVFVGMLLLVLVAGGAVHAAERPLLSEAGTRAESPEPLP
                     TPDAPDAVSNAAESTAPAASEAQPYAAPRFHEKIDLDAIQLEPFYEKLAAKAAPEETL
                     LTLEECIRRGLKSNLDIQIVSFGSLKSAADILAAQGEFDPIGTGSVTYTETEQQASSQ
                     IFAYGGISTIDDYRARGQAGIQGKLHWGTKYDVSMLLEWEENTFNYYIKEWSGGLSLS
                     LTQPLLRGRGKAVNLARVRIAQNAEETAEHQLKIQTMSSLAEIIKAYWDLVGAVEQHK
                     VRLSSMANAERLL"
BASE COUNT         2464 a         3792 c         3828 g         2526 t
ORIGIN      
        1 aagcgcaatt gcgcccgatg gcctgctccg gagcgtagcc ggtgatgcgt tccatggccc
       61 ggttccagcg ctgaatcacg aactccgtat caatgacaaa aaccccgtcc gccatggtgt
      121 cgaggatggt tgccagttca agtgcttcac gttccagcga ttgcgtcatg cgcgtatctc
      181 cgtagctccg gaatcgatca tagcacacgg agatagcaca acgttacctg cctgccatag
      241 aaaaacgcgg cgccccttgc gttccgagcc tggaacgcaa ggggcgcctt cggggtttct
      301 acaccatcga ccgcagccca gcgaatcgat tacaccagtt tacgcagacc acgcagcgac
      361 agggcggcga tgccgagacc catcagggca atggaggtgg gctcgggcac aatcggccgc
      421 accaggatca cgagatcctg ataatcccag tcggcccggg gatcgccagc caggcggtct
      481 tcccacgcca gcaggtaggt gcccggctca tagccttggg gcgcggcgag catgagcatc
      541 tgtacccgct cggcctcatt gcggccgtat tcggaataca gggtggcgcc ggtctgaccc
      601 acgtgcagcg agaaaccgta gggctcgatc gacgcgatca gctgcgcagc cggcgaaccg
      661 cccgcgccgg ggttgacgta gccgcccgtc accgtgaaca ggaagttctc attttccaca
      721 gcgcccggca acaccgggtc atagtagtag aggctgtgat tatacgtggc gtagcgcgct
      781 tccagagcaa cattctgacc gccgaagaag tggaacacgt ccggtccatt gtaatcgtct
      841 ggatcaacgc caacggcctg atacaaggcg tcactggttg taaagcccgt gctgacgccg
      901 aagatcgctt cgtaaatgtc cagaagctcc cattctccgg ggtgtgcggg ctgcacgttc
      961 acgtcagcca cggtaagggc agccgcactg aagctcacca gcacagccac tgccacgctt
     1021 gaaagtactt ttttcatcat tcttcgctcc ttaaccttta tgcctatgcc agttttgttc
     1081 gagctttgga aaaactgccc catgcaattt ttcgcccttc aaccagggac tctgtcgccg
     1141 tgaacccggg gtagatccgg gacgctcaat tgctgtttcc acagcctcag aagacgcagc
     1201 ccgatgtttt ctgttctacc aacccctcac acatacgcaa ggtgcgtgcc aatactcgcc
     1261 gcaaagccta acaagttgaa tttaaattgt ttacacctga gcaacggcgg ctgatgtgta
     1321 cagcgggaag atcagattgc ctgcagaggc attgacattg agtcacggct gacaactttt
     1381 tttacgaatt gggcgccgct gcgcaagccc tgaccggcag ggtaatacac cgcccttcat
     1441 cccaggggct ggatcaccgt acacgtccgc gctttgtacg gatctcccct ggttgttgag
     1501 gggattatgc ggagcatcca tttcagaaaa acttgatcct gagtgccatt ttgggtataa
     1561 attagtgtga ttctgttgtt aagtgaaagt agcctgacgc tgctacctga gcgcacacga
     1621 ggctcgatgg cagtcacgat ggtacggata ggaaggcaac ctggtactcc gccgtgcggg
     1681 agtagcacgg aaaaacccac gcgcatgagt ctggcagatg tcgccgctgg ggcgtggtga
     1741 gggttccagc tgcgccgctt gcctcagagc ggtgaacggg aatgtgatga ggccaacgcc
     1801 aggctttcaa cggaacggcc ccgtggcgcc gctcctgagt ctggacttta ttttggcccc
     1861 agcgggagac ctgacaggca cacgataggc attggggtag ttgcggcgcg gtttttcccc
     1921 gcgatttacg ctgcctgcct gaacactcaa cgacacaagg attcacacgc gaattcatga
     1981 aacagatttg tgcggtatcc gatcttcact tgttctgcaa acgttctgag gcgcatcgtc
     2041 acacggcggc gctcgaccgg gctattgccg aagcggaagt ctgcgtgctc aacggcgata
     2101 ttttcgattt tcggtggagc gtatttgact ccgttgaaga aacggtggat gaagcgcttg
     2161 cgtggctgga ccgcatcatt gcgcggcacc cgcattgcga ttttcattac attttcggca
     2221 accacgacag cgtgaaagtg ttcatcgatg cgctggatgc ttatgcggcc cgcacggagc
     2281 gtctgacctg ccatcacgac ctgttccgct tgaacgccgc ggtgtttctt catggcgatg
     2341 tggcggaccg gaagatgacg cccgcggagt tcgaggccgc gcgccgcgtg tggcactacg
     2401 accgccaacg gggccggata gtgaaccggg cctacgatct ggccgtgtgg ctgggcgcgc
     2461 accgcacgat cccgcgtctg atgttccccc ggcatgttgt tgccggacgc ctcgtgcatt
     2521 atctcgagca tgtgggccac gggccggatt cgggcgtgac cgacgtctat tacgggcaca
     2581 cgcatgtggc agtgcggggt ttccagtaca acggcctcaa cttccacaac agcggcgccc
     2641 ccctgccgaa gctgggcttt accatactgc gcgccacggc atagcgcgga atcgaggttc
     2701 gcgggctcag ctttcgctgc ctttgcgcaa atggactttt ccgttggcga gatgggcatg
     2761 gagcgggccg ggaaactgct cccgaacgtg cagttcttcc tccgcgagcc ccaggcagct
     2821 ctccgggtag accactttca ggatttcgag ccgggcccgg gtattatttt tcgaatggag
     2881 cttcaggtcc tctgcctcgg ccgtcaagat ctggatgcgc cgggccacct cgtccatttc
     2941 ctgttggagc tgagccggat cgtctttttg tgagctgtcc gggcggctca tgggcaagcg
     3001 ccggggcgtg gctaagaatt tgagtttcat ctcaagctgg ccttgctggg cgaaaagttc
     3061 gttaatttcg cggcgaagcg tccggagctt gctgtcgagc cggaaatcgc ggcccgcggt
     3121 cagcaccgta ggcaccaggg cttcgctgcc cacctggccg acgatgaagc cggcccgcgc
     3181 gacagcctcg ccgccgacga tgcgcccgcg cggcacacag atcgaaccca tcgagcagac
     3241 gaacgactgc acgatctccc gctcaacgat gatgtcgtgg ccggcctcca ccttggagtc
     3301 aatgatgtat cgggcctgaa cccggccctc gaccaggatc ttgtattttc cgcgccccat
     3361 gatgccgccc cggatcacga ggttcccgcc ggcctccaca tcggcttgct cgacgatgcc
     3421 actgacttca atatcgcctg tggcgcgcac cacgctgttc ggttcaatgt ccccttcgat
     3481 caacaccgtt ccgagatggt tgatatcgcc cgatttgagg ccgacgttgc cgcggatcac
     3541 aagcacttcg tcgaccgaaa cggccctcat atcatgttgg atgcgcccgt cgcgcgtggc
     3601 gaaatacgcg gtgtcttcct gccggacatt ccggcccgcg cgcagatgca cgctccgccc
     3661 tttttttccg gggacgcgat ttccgtagac atcgacgccg tctgcgccgg gttgaggggg
     3721 atgaactgtg gccagaagct gcccggcctg cacattgcgt tccgcggcgg gtttgcggta
     3781 attgagggcg ccggtctctt catccagtac aaagcctgat ttgaaaaaat cgccggccca
     3841 ttcgatgtat ccatcgaccg gttcgccggg acgatcgcct tcggccagga tcaggccgcg
     3901 ccaggcatcg tcgctgactg cggcgcggat ccgtccgccg attcgttcat ccagcagatc
     3961 cggccggatc tgcaattggc ccaattgccc cagcacccac gcaacgaacg cgtcgagctc
     4021 atgggggatc gagctggcgt cgaggagcac ggccattcga tcccgggaaa cgctcacccg
     4081 gacctcgggg aaggacacat ccggcgcgcc gtccaaatct atgtcgcgac gatcatcctg
     4141 cctcatgcac gcgtttcaat ccggcacgtt tttctgaccc agcctactcc agtatgcaac
     4201 aaaacagggc tcagaacaga ggtcctgagc cccgcgaaca cttgctggca gccgtgtgtc
     4261 ccgctatcgc cggcacctcc gtgtacggac ctcaccatgc atcgcgccgg gatccatctt
     4321 ctggaggcac gcagcagcgt ggcaggcccg cctgccagcg ccgggtcttc ccggacttcc
     4381 catcgatgga ccacctcctt tccacctggc ggaacatccc gcggtcccct catcgggccg
     4441 catcgtcagc gcgaggattt ctctggctaa ctatagacac aattcacctg aagcgcaagg
     4501 gggtttcttg acaacaggtt ccccaaaaga ctttctgttt tgtgggtcca cgtgaaagag
     4561 ccgtttggct gccatccggg ttgcaggggc ggccccctgt ggccgccctg atccacccgg
     4621 cgccaactgc cggcaatcac acggcaacag ccccgtcaaa cccgatatac caccccggaa
     4681 aacaccgccc tcatacacca tctgcgaact tcgctctctt acccgaagat tcggcaaatt
     4741 gcagtttgtg ccaaaagcag gtatcctgtc cctgcgtgtg tgtaaccatc aggccatggg
     4801 cacccatgga caaactcaac aatgtggaga ggtttctatg agcggctcgg gtggcagtca
     4861 cagttgcccg cgttcaacac aggaattcac tcaggaagac gctgaagacc ctggggcgca
     4921 ccctttgcgc ctcgcggcct gtgacgtaca cagccggcac cgccatcaag cctagctcgc
     4981 cggttcgcct ccggccgcgt ccagcgcctt gacgtggccc aatatggagg tggaccatga
     5041 aggatacgaa tctgcccctc tgcccgggac tgcccggggc attcaccgct ggagcggcaa
     5101 cagccgcccg cattccggat gcattcgcct gccccgcctc ccgtgcgcga tgcgcagccg
     5161 cccggaccgg caagaccgcc tcttcgcctg ctcgctctaa tcatttcccg gtttcgccgg
     5221 agcgcgcggc ctttgctcag cgatttctgc tggaggccgg gccttggtac ttccgccgca
     5281 cgcacaagaa gggaatgctt gcgccatgaa aaagatccgt ttcgccaata gttctaacgg
     5341 aaatacggta gcctcctgcg tccagcgggc gcctaccggg catgagcgcc gcctggtaca
     5401 gttgtgcaca gttcctgtcc gggaggctgt agctacactg gaatcgtcgc ttcggggcct
     5461 cagtggcgaa gaggcggagc agcgcctcga cgcattcggc cccaacgaac tcacccacct
     5521 gcagcctctc ggtttctggg gcgatttgct ccgccatttc aaaaatccac tggtgctcca
     5581 actgctgatc atcgctcttg tctcggcagg tatcggcgag cacgcgcccg ccatcattgt
     5641 gggagcaatg cttctgctca gcgtggggct ctccctgata ctcgatcgcc gttccagcca
     5701 ggcggtggag aacctgggca aacgggttca atcccgcacg tttgttctgc gcgacggcgc
     5761 ggaaagggag gttcgcaact cggaaattgt ccccggtgac atcgtgctcc tgcaggcggg
     5821 ttctatcatt ccggctgatc tgcggctgat caccgccaaa gactttttcg tcagcgaatc
     5881 ggcgctgacg ggcgagtcga tgcctgtgga aaaaaccacg gcccttcccg acgaccagaa
     5941 catagccgcc ctggaactgc cgaacgcctg ttttttcggc acatcggtca atagcggcgc
     6001 ggcccggggt ctggtggtca acacaggcgc ctgcaccctg ttcggggcta tcgcggagcg
     6061 gcttgccggg aaacctgagg agaccgattt tgaccgcggc gtacgctcgt ttacctggct
     6121 catgatccgg ttcatggtcg tgatggtggc cgttgtcttt ttcgttgtgg gcatgagcaa
     6181 gggcgactgg gtgcaagccc tgttgttcgg cctctccgtg gcggtcggac tgacgccgga
     6241 gatgctgccg atgatcgtca cggtcaacct ggccaagggc gcgctgacca tggccaagcg
     6301 gaaggtgatc gtcaagcggc tgccatccat ccagaatttc ggcgccatcg acattctgtg
     6361 caccgacaaa accggtaccc tgacccagga ccgggtcgtc ctggaacgtc acgtggatac
     6421 cctggggcgc ccgagcgagg aagtgctcga ttacgcctac ctgaacagtt atttccagac
     6481 cggtctgcgc aacttgatcg accgtgcggt tctggcgtat tccgatctgg acacccgcca
     6541 gtgccgtctg gtggacgaac tgccctttga cttccaacgc cgccggatgt ccgtggtcgt
     6601 ctcctatgaa gacgaccatg tgctgatttg caagggagcg gtagaggaga tttacgcgtg
     6661 ttgcagccga taccagttcg aagagcaggt ttatccgctg gtcgatatga tccgcggggt
     6721 gctctttgaa gaggtcgagg cactcaaccg cgatggattt cgagtgctcg gcattgccta
     6781 tcgggagttt ccgagcgata agacggtctt ctcgattgaa gatgaatccg agcttgtatt
     6841 actgggatat atcgcctttt tcgatccgcc caggacgtcg tgcgcggaag ccttgaaacg
     6901 gctcgccggc gccggcgtcc aggtcaaggt gctcaccggc gacaacggtc tcgtgacgga
     6961 gaaagtctgc cgtgatgtcg ggctggcggt ggagcagatc gtaacgggta ccgaactggc
     7021 ccacttgtct ccggagcaat tctcgagcgt ggtgctggat gccaacgtct ttgtgaaact
     7081 gtccccggca cagaaggaag agatcgtcga agccctgcgc cgcgacggcc acgtggtggg
     7141 attcattggc gacggcatca acgatgcgcc tgccctcaaa gcggccgatg tcggcatttc
     7201 ggtggattcc gcggtcgatg tggccaagga agcggctgat atcgtgctcc tggaaaagag
     7261 cctgctggtg ctggaagaag ggatcatgga agggcggcgc gttttcgcca acatcgtcaa
     7321 atacatccgc atgggcgcca gctccaactt cggcaacatg ttcagtgtgg tgggagcaag
     7381 ctacctgctt ccgttccttc cgatgcaacc ggttcagatc ctcatcaaca atctcctgta
     7441 cgatttttct cagaccggca tccccacgga cacggtggac gacgaattga tcgccaaacc
     7501 gctcaaatgg aacattggaa acatcaaacg attcatggtc tttatcgggc cggtcagttc
     7561 ccttttcgat tatgcaacct ttgcgttgat gtggtttgtc tttcaatggg gcgccgtttt
     7621 cgattccagg ctgtttcaga ccggctggtt cgtggaatca ctgctcacgc agacgctgat
     7681 tgtccatatc atccggacaa aacgcgttcc gtttttcggg agccgggcct cgctgcactt
     7741 gacggtgacc acgctcatca tcatggccat tggcatctgg ctgccgtatt cgccttttgc
     7801 gagcgatctg ggaatgatgc cgctgccggc aatttattgg gcctggatcg cgctttttct
     7861 tacggcctat gccgttttga cccacggggt taaaacctgg ttcttcaagc gctatggagg
     7921 ggattgatca tggatacgat tctggatgca ctccaggaag ggcggctttt cgagctgccg
     7981 gataacgaca aaacggatgc gcttcagttc ctggcccaca tcatcgaagc gttccctgag
     8041 gtacccgccg gaacggacgt cgtggggaat gtcctgaaaa aagaggaggc agccaatacc
     8101 gcgctgggca agggatgggc ctgtccgcac gcgcacgtgc ctttcgatga agacctcatg
     8161 tgcgtgatcg gctggagccc cgcgggcatc gattacggcg ccacggatgg aaagcccgtg
     8221 accatcgcga tcatgtacct ggtgccggaa aaccagcgca gacactacct gcgcgagatt
     8281 tcgctgctgg ccaaagccct ccagacatat cccgggctcg ataagctgca ggaagtgaag
     8341 gatctcgatg atatccgcaa ttacctgctg gatctgatag attcttcgaa agagtcggcg
     8401 ggactggaca cccgggcgag gatgatccgc ttgcaggcca aaccagcgcc ggaattgccg
     8461 gttctgcagg atttgtcgaa cctgctggta gaatccgtca ccctggttgc cgggccgagc
     8521 ttgaaacctg ttgtacttac gcaaaacccc gatcttctca aatggctgga atccgcagac
     8581 gatttggcgg gaaaaatcga ggttgatggc tcgtaccaaa acggaggctg gcggctcgtg
     8641 cggcgtggag cggttgccta tcaggggaac cgcctcgttt ttgactgcat cgccgttaag
     8701 ctggtcgctt cgtgatacct gggcctcaga ggggcggaga cttcgttctc cgtgcaaccg
     8761 gccggacgct caccgatggg gcccgtttcg cgaaaccggc aatggcttcg caacgggcgt
     8821 ttgaacggca acgcaagcgt ttgctatact tcgacggttt tgctgcggag aaaccggcgg
     8881 ggcttcttgg attcgtcagg gcgatttgcg ccggcccgca ggctgcagtt gaggagtggc
     8941 cgtgatcgag cgaacatttg tgatggtgaa acctgacggc gtgggccggc ggctcattgg
     9001 tgaagtcatc ggccgctacg aggcaaaagg cctgaagctg gtgggtctca aaatgcagat
     9061 cctgtcacgg gcccaggccg aagcgcacta cgccgaacac cgcgaccggc cgttcttcgc
     9121 tggactgatt gcgtttatca cgtcggggcc caccatccag atggtatggg agggggaaga
     9181 cgcgatagcc gcggtgcgca aggttaacgg cgcaacggat tgccgggatg ccgatatcgg
     9241 cacaattcgc ggtgatttcg gcctgactaa gcaaaaaaac attgtgcacg cctcggatgc
     9301 gcgggagacc gccgaacggg aaattgcact gtatttccag ccgtcggaat tgtgggacta
     9361 ccggcagccc ggggaagcct ggctgcagga gtagcggacg gctgggctgc ctcgcatcat
     9421 tacacgagtc tggagacgaa aggaggccat tgcatggctg gtggacgcaa ggttcgtcgc
     9481 gcgaagatcg ccaaacacaa gcgcaaaaag aagcgtcgcg cgaaccgcca caagaacaag
     9541 tagcacgcgt atggcggcgc gatttgggtg tcatgccaac tgaccgcggc cccggtttcc
     9601 gggtccgcag gctctggtgg cagtgtctgc ccggatcgcg gccgtgcagc gcaggaggtg
     9661 agcattcatg gcggatgaag acaaaaagat cttcatagac gaagactgga aagcccaggt
     9721 cgcccgcgaa aaagaagaag cccgcgccaa gtccgcacag cagagcgaac ctgcttcgga
     9781 gacagacgag gccggctctc ctcaagggga tcagcccggc gcggatacca tcgaacccaa
     9841 tttcagcggt ctggtggaca gcctggccat gcagggcctg atggccctgg gcctcatcgc
     9901 tccccaggat gcgaaagaag tgtatgtgga catcgcgtcg gcgcggtttg tcatcgacac
     9961 gctcatgatg ctgcgggaga agaccaacgg caacctgacc ccggacgaaa cggggcatct
    10021 cagcagcgcc ctgggtgaat tgcagcggtt ctacgtggcg cgcgctcagc aggttcagga
    10081 gcaggccatg aagcaggcag gcgtcgacat gaacaacctg agggcgccca ggcaataagg
    10141 ggcggcgtat ggcgcctgcg tcgccagtgg ccagccgcac agatgaggac ggtgccatag
    10201 agcggttttt tcagctggct ggaagtactg cctggccgcc cggtgtgaat gaagccgcgc
    10261 cggatcattt gtggctgtca tgagactgtc ttgtgcgaat ttgtgtaaac gcaacgcccg
    10321 gctccacggc cgggcgttgc tgtttctggc ggcagcgatc ggaggctgcc tggcaagccc
    10381 ggaaccactc agcccggacg aagccctggc acgcgcgcac attcagatca cccgcctcct
    10441 tcaggaggag ctgacgcggt atccggaagc catcgacatc gaagcgcctg aggccgtcga
    10501 taacccggcg gctgtcaccc tctggtaccc cgcgcattcg atcatcgcgc ccgcactggg
    10561 gaatccggag attcggcgtg cgtttgacgc ggcacatcca ggcatcgagc tgaaacccca
    10621 gttcatcggt gactggcacg tggccattca gaagttgacg gtgagcattg cagcgggcgg
    10681 cctgccggat atcgcgatcg tcgagcgcag ctgggcagcg cgcctggccc aggccgggcg
    10741 cctcatgcct ctggatggct tcctcgatgt ggagatgctc gacgactttc acccgaacgc
    10801 ccgcgccgca tacaccctgc agggacggat ctgggctttg cccgcggatg gcttttgctc
    10861 tgttttgttt ttcaaccggg ctgcagttgc tgacccgccg aaaacctggg aagaactccc
    10921 cgtccaaatc tccgaaacga aggctgtgat cggccatttg ccctatattg agatgctgtg
    10981 gtcggcggga gggcgggtct gtgagcacaa ccagagcggc ctgacagcac cctccgccat
    11041 gcgcgcgctt cattttcttc tctcgctccg cgataccggc cagtcggacg cgcaaatgct
    11101 cgccgacccc gcctggggct tcgctgcgtt tatgaagaat agcgccccca tgacggcggc
    11161 ctcatcggaa tggcttgtgc gcacgcgaga cgccgccttc cccgcgggta tggcagccct
    11221 tccgggcgca gaaggtccga tttcacgcgt gggcgacatg gcgatcgtgg tttttgcaaa
    11281 acatgcggcg gcaaaacacg atgccatcgt ggcagcgctg gattggctga ccggccccgc
    11341 agtgcagggg gccgctgccg cagcccgagg ttcgatgccg gtgcgccagt ccgtagcgga
    11401 aaccgtggat atccccccgg ggttgcgtgc ggcctatacc gcggcacagg cgcctccatt
    11461 cgccggcgtc tggagcgccg ccgaatatga attgtcgagc aacctcgctc gcgtctggca
    11521 gtggaaacac gaatcgccag aatctgaagc ctcccgccat ggcggcactt gggaaacaag
    11581 tccatcgggc aattgactgg gcgggcgggg ataattacac tcagcgggcg ggcggccggc
    11641 gtgcggaagg gccgcctgct ctggaaggcc gcggttcgtg aaaccgtggg tgtgtctggc
    11701 gcgtcctaat accatgcagc gaaccggtca gaggggagtc ggatgaacat cctgtatcgc
    11761 gtgtttgtag gaatgttgct gttggttctg gtggccggcg gcgccgtgca tgccgctgag
    11821 aggccgctgt tgagtgaggc gggaacgcgg gcagagtctc cggagcctct gccgactccg
    11881 gatgcgccgg atgccgtttc aaacgcggct gaatccactg caccggcggc atctgaggca
    11941 caaccctatg ccgcgccgcg ttttcacgag aagatcgatc tggacgcgat ccagctcgaa
    12001 ccgttctacg aaaagctcgc cgccaaggcg gcccctgaag agacgctgct cacgcttgaa
    12061 gaatgcatcc ggcgcggcct caaatccaac ctggacattc agatcgtatc gtttggctcg
    12121 ctgaaatcgg ccgcggacat tctggctgcg cagggggaat ttgaccccat cggcacgggc
    12181 tcggtcacgt acaccgagac cgagcagcag gcgtcttcgc agatcttcgc ctacggtggg
    12241 atctccacca tcgacgacta tcgtgcgcgc ggccaggccg gcatccaggg gaagctccac
    12301 tggggcacga aatacgatgt gtccatgctc ctggaatggg aagaaaacac gttcaactac
    12361 tatatcaagg aatggagcgg cggcctctcg ctttccctga cgcaaccgct cctccgcggg
    12421 cgcggcaagg ccgtcaacct ggcccgggtg cgcatcgcgc agaatgccga ggagacggcc
    12481 gaacatcagc tcaaaatcca gaccatgagc tcgctggctg agatcatcaa ggcgtattgg
    12541 gacctcgtgg gtgcggtaga gcagcataag gtgcgcctga gcagcatggc caacgccgaa
    12601 cgcctgttaa
//
LOCUS       DAOPBQ010000035        12484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_5013_length_12484_cov_4.716952, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000035 DAOPBQ010000000
VERSION     DAOPBQ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12484
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5013_length_12484_cov_4.716952"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PKW60_02245"
     CDS             complement(<1..250)
                     /locus_tag="PKW60_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98282.1"
                     /translation="MRMGRREFLGASASAILVAGTFGRGTVFGANERIRAGIIGLRSR
                     GTYLIQALSKVDGFEVGAICDVDQNVLEACAAWHQKNTN"
     gene            complement(412..2064)
                     /locus_tag="PKW60_02250"
     CDS             complement(412..2064)
                     /locus_tag="PKW60_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HPJ98283.1"
                     /translation="MPESFGAIVRKLRRLAEYTGPWRPLRDEAPRLRTSVAELRERET
                     RLDDLLVVALVGGSGVGKSTLLNAIAGDQLAQTSEFRPCTATPTVYHPPGARFEAGDW
                     RMVSGSALEHLVIIDTPDSDTVIKEHRETVEKVLAACDLILICGSPEKYLDEATWSLL
                     RPLQGERTMACVETKARAEMPSVREHWLSHLAEQGFTIEGYFRLNARATLDRRLQGAR
                     ASEFDWPAFEAFLREELTREKVQRIKRSNAIGLLTKTVRDLQERVGARAPELTALEKS
                     LRESRQALAADTAGIVRRRLFAERHLWAYALSREIDVRALGIVLTLYRLFDAIRSLPV
                     RLAGWLPGSRKTRLGRQAAALLSSVNLFQEDLVLVSDAIQQQYESHRSSLAMAFAHAG
                     FDELDDSTGYDAYVNALGKRIADVLRGPARDRVVAHAHRLTSWPVALLLDVGPIAFLA
                     YTCYHIVVTYFDAQLLSAGFLMHSVVVLAIIVTAELLVLSLLMRMGAWAARNAAARDL
                     RHALKAPGKNALGFEPEAAALADARAQAEAVRSLVADVLDGD"
     gene            complement(2037..3836)
                     /locus_tag="PKW60_02255"
     CDS             complement(2037..3836)
                     /locus_tag="PKW60_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPJ98284.1"
                     /translation="MKTQAIESRDPLGAGLELCERSLNALENALRPDRVLTETIFDGT
                     SDWVNLLRYKLGPHLSGRGCLLAAVAGGTNTGKSTVFNLLQGREVSPVRPTAAATCRP
                     LLSGSPERARQCLEGKLVPEFTPAPLDNPEALLTRETPADQLFVVSNPELPDRLVLLD
                     TPDVDSIEKQHWTLAGHLRAAGDVIVAVVTGEKYRDERVVAFFREAQRSGRLVVPLIN
                     KADTAEEFATARRQLDEFCREVGCTGPAFAVPHNFRLAQDYWAPIQSLDGAEDLMGYL
                     LSLDAHAIKRRVFESSVRHLCERAESFLNDVERVAEMLASVREEYHSRALHAAQQYDP
                     APGAAVGGLFHEFVQTRRGPFRRAIGQAGAALARGTAAVGRSISRAVFRRATLESPEE
                     SPSRAEIHAAHRQTIEQITRNLAAEYIQSSKNLREPAAHLLQAGARQLSPETVTEQVV
                     QAVLQSDDLSEAFRSHAHRTLEAWWNDRRGRRHVLEALDTILAVMPAAIAVPMSIYTA
                     GVGVPEAMVVAGPLAEQFVARVIEYQFGDALFDFLSPWRDEQQRHLAEALLEQVAAPL
                     LAGLDAMLAVLEGGAVRELRKGTELCLKALGPS"
     gene            4049..4600
                     /locus_tag="PKW60_02260"
     CDS             4049..4600
                     /locus_tag="PKW60_02260"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPJ98285.1"
                     /translation="MRKANLEHDSDAALVWRWRAGNGYAAAAIVERYADAIGAIAYGV
                     VRNRALAEDAVQETFARAARTIGKLNDPKRLGPWLVGIVRNVAVDMLRRRARELPIGE
                     YDIASKGDPSREAMRNEAANALRDALAALPEDQRDIIAMKYVAGMSYADIARVLGISP
                     EAVSQKLWRVRQKLQSELLEFRP"
     gene            4597..7509
                     /locus_tag="PKW60_02265"
     CDS             4597..7509
                     /locus_tag="PKW60_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPJ98286.1"
                     /translation="MNPKDSERLSRYLDGELTPTELREVESLLATSLEAREEYAVMKQ
                     VHKSVRDILPETRFSRPELAAPRRRSLHWLAAAAAIVVLFGGLMFGVQVYHGSFKEPA
                     APPTSAAPSPVSPVAPAPGTLAGSPAPSTGAPAMPATPGMEADASEAEHPSASFLKGV
                     VTNSQNEPIPGASVRALAWLDPSPPYGLDGDPLLASVHTDGGGRFLLDWPSEARAVAI
                     EARGYAPRHWHYSGGVLASALELHAQLQPEEPDTGRVVDSTGAPLAGVRVAAQSACDP
                     AQAAVTGADGVYRVSSWQGMGPLFFSHPDFALSWTKPDASEATETVLEPGGALRLCVV
                     QNGQPVPGAMVQMVAEIPNALGYALRREADEAGCVEFEQLPTDPAALENFAVVATAPN
                     GVKARLEEVPAIRAGATAECVLTVPDSYPGKVAGTVKDTEGNPLAGVAVVARSRGAVS
                     QCIRTDENGAYTIGLPLGDTRISMAWDFLGPGQDVLEPGRNVLQVNKAGTTFTQDFTL
                     ETSVKDITFVRTDGAPVEDLWLQLEPGLPRDYDRSARWRVAASAGHIQVLCKSIERGV
                     AYDPVGEYAATWSMPEDQAQATVVFDLPVGALTGRIIDTAGQPLRGASLYARGSTQDY
                     RFEQAWSGAEGAFRIEPLSLDGTYDLYIYLDGYQPLPGSHLTGLRAAKEAAPLEIVMA
                     PRDAGVSGQVLASDGSPLYHAHLFLTDSERTGHHASTGQDGAFSFEVAKGVYTLWAIA
                     PALDATTSVTVEAPSHDTVITLPVAASPPPVADVAPPHDPASEKAVNDMKQLGLVFKM
                     FANEAPGEFFPALEPQPGVLAPEWNAIYPEYLVDTRIMNTQEGVTRCYFAHALTGEAA
                     GLAFLDALQQLGIEAIRDQDIQVGPGKGSAGGDVILRLHEGFESRLLTEVPPNVSGYL
                     VQAATPVLWEVPGGREESGGWVMYMDGRVEWVPYPGPFPMTEAFVTRTRDVLGLGR"
     gene            complement(7633..8946)
                     /locus_tag="PKW60_02270"
     CDS             complement(7633..8946)
                     /locus_tag="PKW60_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HPJ98287.1"
                     /translation="MSNDRQNTVEAARPPWWKTIGPALITACVVFGPGSLVVSANVGA
                     TYGYELLWLLVLAGLVMGGYMTMSARVGVTAGSSHFSTLAREVNRPFAALLGIVLCLT
                     CASFQFSNNLAVTLAVSAFAPERYSLAFQIGAVGLLNAVLILFLFRAHSVFKTIENVM
                     KFMVGLILLSFLVNLLVARPSVLGILKGLVPGIPKELSLGVPKIVDGTINDPLVLIAS
                     LMGTTFSVAGAFFQGNLVREKGWDTDDYDRGIGDSVAGVGVLTLVSMMIMITAGTVIL
                     GKPANNIATLAQTLHPLLGPTAFTLFCIGLVPIALNPFLINAMIGGTALADGLGFPGK
                     LNDFWPRMFTICVLLTGFVIASVGLLLKIPTVNLMIFGQALTVVGNPLMAATMLWLAN
                     RQSVMGDRRNGLAGNIIGVLGIIVVLLLALRMLWFLILHLSLALS"
     gene            complement(9197..9487)
                     /locus_tag="PKW60_02275"
     CDS             complement(9197..9487)
                     /locus_tag="PKW60_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98288.1"
                     /translation="MKKETEDRLLDLAGILIASVILFVIGFGAGLWIGLDILPFEMLW
                     ESPVPLLIIGLIVGAFAACLPFTINYIRHWDYIQQRNIPRDPESQPETKIDP"
     gene            complement(9615..10034)
                     /locus_tag="PKW60_02280"
     CDS             complement(9615..10034)
                     /locus_tag="PKW60_02280"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPJ98289.1"
                     /translation="MFTYPVRISLRDGDAAGVLFFGRYFALAHDAYETFLNSHGLDIG
                     HLIRTAGYLIPVVHAESDYRCPLFVGEQALIRLRVAELRSRTFTLDYEFHTAEGRLAC
                     RMRTVHCAVNAETRRAVKLPPDVVRALKSELEPGLSV"
     gene            complement(10063..11331)
                     /locus_tag="PKW60_02285"
     CDS             complement(10063..11331)
                     /locus_tag="PKW60_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020616980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HPJ98290.1"
                     /translation="MTDSETLFSRDTLDEMRAAVHETVDRTPVTDIHTHVYAPAFGGL
                     LLWGMDELLTYHYLIAEVIRASRIEPAAFFAQSKREQADFIWRTLFIERSPYSEACRG
                     VLTVLDRLGLDVASRDLAAYRDYFARKSVEDYINTVFERTNLESVVMTNDPFDDAERP
                     VWEAGAAMDKRFHAALRIDPILNDWTGACARLQGWGYNVDERLSGTALAEVRRFLTDW
                     LQKTRALYMAVSLPQDFAYPENSARARLIDECIIPVAKEQHVPFAMMIGVKRAMNPAL
                     KLAGDGVAKSDAGAVERLCAAYPDAKFMVTMLARENQHELSIAARKFPNLFLFGCWWF
                     LNVPSLIEEMTRMRFELLGTSVLPQHSDSRVLDQVLYKWSHSRAIIARVLADKYADLL
                     RTGWTLKQSEIDRDVAGLLGGNFWDFLKQA"
     gene            complement(11424..>12484)
                     /locus_tag="PKW60_02290"
     CDS             complement(11424..>12484)
                     /locus_tag="PKW60_02290"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98291.1"
                     /translation="GRKYGISDAMLFPDYREMLALSELDAVIISSPDHWHALHSIHAM
                     EAGKDVYVEKPMTLTIREGRLMVDAARANGRICQLGSQQRSSQKNRVGCALVRAGRIG
                     NVHTVHTANYPSPWECSLPEEEAPDYLDWDMWCGPTPYRGYHEKLYMPRGGKQDHEWG
                     WISFRPYSGGEMTGWGAHGLDQIQWALGMDESGPVEIWPVLDEEPPFDGVHLGPRWPV
                     HMRYANGVVVKLDDQGAPGGGWFEGDEGEIKIDRDRYAIKPGSLDKELPELEPIPFTG
                     GTDEHIGNWLHCVRTRQTPNADVEIGHRSTTVCHLGNIARWLKRSLTWDPVTETFPGD
                     DEANRLVDRERRVPWTLG"
BASE COUNT         2306 a         4059 c         3884 g         2235 t
ORIGIN      
        1 cattcgtgtt cttctgatgc caggctgcac aggcttcgag cacgttttga tcgacatcgc
       61 aaatcgcccc cacctcaaag ccatccactt tggagagcgc ctggatcaaa tacgtgccgc
      121 ggctccgcaa gccaatgatg cccgcgcgga tgcgttcgtt cgcaccgaac acggttcccc
      181 gcccgaacgt gcccgcaaca agaatcgccg acgcggatgc ccccagaaac tctctgcgtc
      241 ccatgcgcat acccgaatac ctccttgccc tgacaattca tgcctggttt cgcggcagcc
      301 accggctacc gcccgcgcgg tcaacattgc gccgctgcga tgccgttgtc catcgtagca
      361 tctccgccgg gttgagatca cggtgccgcc tgagccgccg ttcagtcctg ttcagtcgcc
      421 gtcaagcaca tcggcgacaa gcgagcgcac ggcctcggcc tgggcgcgcg catccgccaa
      481 cgcagcggct tccggctcaa aacccagcgc gttcttgcca ggcgctttca atgcgtggcg
      541 cagatcgcgc gccgcggcat ttcgtgccgc ccacgcgccc atgcgcatga gcagcgaaag
      601 caccagcagt tctgccgtca caatgatggc cagcacgacc acggagtgca tcaggaatcc
      661 ggcagacaac aactgagcgt caaaatacgt caccacgatg tggtagcagg tgtaggccag
      721 aaaggcgatg ggcccgacat ccagcagcag cgccaccggc cagctggtca gccggtgggc
      781 atgggcgacg acgcgatccc gggccggacc gcgcagcaca tcggcgatcc gcttccccag
      841 cgcattcaca taggcatcgt agcccgtgct gtcatcgagt tcatcgaatc ccgcgtgcgc
      901 gaacgccatc gcgaggctgc ttcggtggct ttcgtattgc tgctgaatcg catcggatac
      961 cagcacgaga tcttcctgaa acaggttaac gctcgaaagc agcgcggccg cctggcggcc
     1021 caggcgggtt ttgcgcgagc ccggcagcca gcccgccagc cgcacgggca gcgaacgtat
     1081 ggcatcaaac agccggtaca gcgtcagcac aatgccgagc gcccgcacat cgatctcccg
     1141 gctgagcgcg tacgcccaca gatgccgttc ggcgaagagg cgtctgcgca cgatgcccgc
     1201 cgtgtcggcg gccagggcct ggcgcgattc gcggagtgat ttctccagcg cggtcagctc
     1261 cggcgcccgt gcgccgaccc gctcctggag atcgcgcacg gtcttggtga gcagtccgat
     1321 ggcattgctg cgcttgatgc gctggacctt ctcccgggtc aactcttcgc gcagaaacgc
     1381 ctcaaacgcg ggccagtcaa attcgctcgc ccgggcgccc tgaagccgcc gatccagcgt
     1441 cgcgcgcgcg ttaagcctga aatagccttc aatggtgaag ccctgctcgg ccaggtggct
     1501 caaccagtgc tcccggacgc tcggcatttc cgcgcgcgcc ttggtttcga cgcaggccat
     1561 ggtgcgttcg ccctgcagcg gccgcagcag cgaccaggtg gcttcatcga ggtacttctc
     1621 gggactgccg cagatcagaa tgaggtcgca cgccgccagc accttttcca ctgtctcccg
     1681 atgctctttg atgacggtgt ccgaatccgg ggtgtctatg attaccaggt gttccagcgc
     1741 cgagcccgac accatgcgcc agtcgcccgc ctcgaagcgc gcgcccggcg gatggtacac
     1801 cgtgggcgtt gccgtacagg gccggaattc cgaggtctgt gcgagctggt cgcccgcgat
     1861 ggcattgagc agagtggatt tgcccacgcc ggagccgcct accagcgcca ccacgagcag
     1921 gtcgtcaagc cgcgtctcgc gttcgcgcaa ttcagctacg cttgtccgca ggcgcggcgc
     1981 ctcgtcgcgc aacggacgcc acgggccggt gtattcggcc aggcgccgca gttttcttac
     2041 gatggcccca aagctttcag gcacaactcc gttcctttcc gtaattcgcg gaccgcgccg
     2101 ccttcgagca ccgccagcat ggcatccagc ccggccagca gtggcgcggc cacctgttcg
     2161 agcagcgctt ccgccagatg ccgctgctgt tcgtcgcgcc atggcgacaa aaaatcaaac
     2221 agggcgtcgc cgaactggta ttcaattacc cgcgccacaa actgttcggc gagcggtccc
     2281 gccaccacca tcgcctccgg cacgcccacg cctgccgtgt agatcgacat gggcactgca
     2341 atggccgccg gcatcaccgc aagaatggtg tccagcgctt ccagcacgtg ccgccgccct
     2401 cttcggtcat tccaccacgc ttccagggtg cgatgcgcat ggcttcgaaa cgcttccgac
     2461 agatcgtcgg actggagcac cgcctgcacg acctgttcag tcacggtttc gggcgacaac
     2521 tggcgggcgc ctgcctgaag gaggtgcgcc gctggttcgc gcagattttt gctcgattga
     2581 atgtattccg cggcgagatt ccgggtgatc tgctcgatgg tctgacggtg tgccgcgtga
     2641 atctccgcac gggacgggct ttcctcgggc gattccagcg tggcacggcg gaacaccgcg
     2701 cgcgaaatac tgcgtcccac ggctgccgtg ccccgcgcga gggccgcgcc cgcctgacct
     2761 atcgcccgcc ggaacgggcc gcggcgcgtc tgcacaaact catgaaacag gccgccgacc
     2821 gcggcgcccg gcgccgggtc gtattgctgc gcggcgtgca gcgcccggct gtggtactcc
     2881 tcgcggaccg acgccagcat ctctgccacc cgctctacgt cgttaaggaa tgattccgcc
     2941 cgttcgcaca ggtgccgcac cgatgactcg aagacccggc gcttgatggc gtgggcgtcc
     3001 agggagagca gatagcccat caagtcctca gcgccgtcga gcgattggat gggcgcccag
     3061 tagtcctgcg ccagtctgaa gttgtgcggc acggcgaagg ccgggccggt acagcccacc
     3121 tcccggcaga attcgtccaa ttgacgccgg gccgtggcga actcttcggc ggtatccgct
     3181 ttgtttatga gcggaacgac caggcgcccc gatcgctgcg cttcgcggaa aaacgccact
     3241 acgcgctcgt cgcggtactt ttcccccgtg accacggcca cgatcacgtc ccccgctgcg
     3301 cggagatggc ccgcgagggt ccaatgctgc ttctcgatgg agtcgacgtc cggcgtatcc
     3361 agcagtacca ggcgatcggg cagctccggg ttcgacacga caaagagctg gtctgcgggc
     3421 gtttcccgcg tgagcagggc ctcggggttg tccagcgggg ccggagtgaa ttctggaacg
     3481 agcttgccct ccaggcattg gcgggcacgc tccgggctgc ccgacagcag cgggcggcag
     3541 gttgccgccg cggtggggcg aaccgggctc acttcgcggc cctgcaaaag gttaaacacg
     3601 gtcgacttgc ccgtatttgt cccgcctgca accgcagcca gcaggcagcc ccggcccgac
     3661 aggtgaggcc ccagtttgta gcgcagcaga ttgacccaat cgctcgtacc gtcaaatatc
     3721 gtttccgtca ggacccggtc gggccgcaac gcgttttcga gcgcgttcaa gctgcgctca
     3781 cagagctcca gccctgcgcc cagagggtcg cggctctcta tagcctgcgt tttcatagtg
     3841 gcttcagtgt acccacaaga cgcgccgtta ccaaaccggc ctcctcggag agcttcatgg
     3901 ctgctccggc gtgcaccgcc atgccggcac gcgttgcatg ggcgagcaaa cggaaatatg
     3961 atgagctcgc caggcgggcg attttgagga aaaactcatg gattccgtaa gaaaaccccg
     4021 tctcgtgcga cagtactgac aggatgtcat gagaaaggcc aatctcgaac acgacagcga
     4081 tgcggctctg gtctggcgct ggcgggcagg caacgggtat gcggccgcgg ccatcgttga
     4141 acgctacgcc gacgccatcg gcgccatcgc ctatggcgtg gtgcgcaacc gggccctcgc
     4201 ggaggacgcg gtacaggaga cctttgcccg cgccgcgcgt acgatcggaa agttgaacga
     4261 tcccaaacgg ctgggcccct ggctcgtggg tatcgtgcgg aatgtggccg tcgacatgct
     4321 gcggcggcgc gcgcgcgaac tccccatcgg cgagtatgac atcgcgtcga aaggcgatcc
     4381 ctcccgcgag gccatgcgta acgaggcggc caacgccctg cgcgacgccc tggctgcctt
     4441 gcccgaagac cagcgcgaca tcatcgccat gaaatacgtg gcggggatgt cgtacgcgga
     4501 tatcgcccgc gtgctgggca tcagccccga ggcggtgagc cagaagctgt ggcgcgttcg
     4561 ccagaaactg cagagcgaac tgctggagtt ccgtccatga acccaaagga ttcggaacgc
     4621 ctgtcccgtt atctggacgg tgaattgacc ccaactgagc ttcgcgaggt ggagtcgctg
     4681 cttgccacgt ctctcgaagc ccgtgaggag tatgcggtta tgaagcaggt acacaagtct
     4741 gtgcgcgata tactgcccga aactcgattt tcccgtcccg agctggcggc cccgcgccgg
     4801 cgttccctgc attggctggc ggctgcggcg gccattgtcg tgttgttcgg cggccttatg
     4861 ttcggcgtgc aggtctacca tggttctttc aaagaacccg ccgctccgcc cacgtctgct
     4921 gcgccgtccc ctgtatcgcc tgtggccccc gcgccgggga ccctggccgg cagtccggcg
     4981 ccttcgaccg gggcgccggc aatgcccgcc acaccgggca tggaggcaga cgcctccgag
     5041 gccgaacacc cttccgcctc atttctcaaa ggcgtcgtga ccaatagcca aaacgaaccg
     5101 atcccggggg ccagtgtccg cgcgctggcc tggctggatc cgtcgcctcc gtatggcctg
     5161 gacggcgacc cgctgctggc gtctgtccac accgacggag gcgggcgttt cctgctggac
     5221 tggccgtccg aggcccgtgc agtggcgatt gaggcgcgcg gttatgcacc caggcactgg
     5281 cactattccg gaggggtgct tgcaagcgca ctagagctgc acgcgcagct gcagccggaa
     5341 gagccagaca ctggccgcgt ggtggattcc acgggggccc ccctggccgg cgtgcgggtt
     5401 gccgcccagt cggcgtgcga tccagcccag gcagcggtaa ctggagcgga tggggtctac
     5461 cgggtttcat cctggcaagg tatgggccct ctgttcttct cgcacccgga ttttgcgctc
     5521 tcctggacga aaccagacgc ctcggaggcc acagagactg tgttggagcc gggcggcgcc
     5581 ttgcggctgt gcgtcgtcca gaacgggcag cctgtgcccg gcgcgatggt gcagatggta
     5641 gctgagattc ccaatgccct cggttatgcc ctccggcgtg aagccgacga ggccggctgc
     5701 gtggaatttg agcagttacc caccgatccg gccgcgttgg aaaatttcgc cgtggtcgcg
     5761 acggcaccca atggcgtcaa ggcgcggctc gaggaggttc cggccatccg ggccggtgcg
     5821 actgctgaat gcgtgctcac cgtgccggac agctatcccg gtaaagtcgc cggcacagtg
     5881 aaagacacgg agggcaaccc tctggcgggc gtcgccgtcg tggcccgctc ccgcggcgcc
     5941 gtatcgcagt gcatccgaac ggacgaaaac ggcgcctata ccattggact cccgctcgga
     6001 gacacccgaa tctcgatggc gtgggacttt ctcggaccgg gacaggacgt gctggagccg
     6061 gggcgtaacg tcttgcaggt caataaggca ggcacgacgt ttactcagga tttcaccctg
     6121 gagacctcgg tcaaagacat cacgttcgtc cggaccgatg gcgccccggt cgaagatctc
     6181 tggcttcagc tggagccggg cctgccacgg gattacgacc gcagcgcgcg ctggagagtc
     6241 gcggcatccg cggggcacat ccaagtgctg tgcaagagca tcgagcgcgg cgtagcgtac
     6301 gatccggtcg gcgaatatgc ggccacctgg tctatgccgg aggatcaggc acaggctacc
     6361 gtcgtcttcg atcttcccgt cggcgccctc acgggccgca tcatcgatac cgcaggccag
     6421 ccgctgcgcg gtgcaagcct gtacgcccgc gggtccaccc aggattaccg cttcgaacag
     6481 gcctggtccg gcgcggaggg tgccttccgc attgaacccc tgtctctgga cggtacatac
     6541 gatctgtaca tttacctgga cgggtatcag cccctgccgg gctctcacct aacgggcctg
     6601 cgggccgcca aagaggccgc cccgctggag atcgtcatgg cgccgcgcga cgccggagtg
     6661 tccggccagg tgcttgcctc cgatggctcg ccgctgtacc acgcgcacct gttccttacg
     6721 gacagcgagc gcaccggaca ccacgcaagt accgggcagg atggggcatt ctctttcgaa
     6781 gtcgcgaaag gcgtgtacac gctgtgggcc atcgcccccg cactggatgc cacaacctcc
     6841 gtgacagtcg aggcgccctc ccacgacacg gtgattaccc tgcccgtcgc agcgtcgccc
     6901 ccgcccgtcg ccgatgttgc gccgcctcac gatcctgcga gcgagaaggc ggtaaatgac
     6961 atgaagcagc tgggcctggt attcaagatg tttgccaacg aagcgccggg tgagttcttt
     7021 ccagcgcttg aacctcaacc gggcgtgctt gcgcctgaat ggaatgcaat ctatcccgaa
     7081 tacctggtcg atacgcggat aatgaataca caggaaggcg tcacccgctg ctatttcgcc
     7141 cacgcattga ccggcgaggc cgctggactc gccttcctgg atgcccttca gcagctgggc
     7201 atcgaagcga tccgcgatca ggacatccag gtcggccccg gcaaaggttc cgcgggtggc
     7261 gacgtcatcc tgcgcctcca cgagggcttt gagtcccggc tgttgactga agtgccgcca
     7321 aatgtctccg ggtacctcgt tcaggccgct accccggttt tgtgggaagt gcccggcggg
     7381 cgtgaggaat ccggcggttg ggtgatgtat atggacggac gcgtcgaatg ggtgccgtat
     7441 cccgggccgt tcccgatgac cgaggcgttt gtgactcgca ctcgcgacgt tctgggtcta
     7501 ggccggtagc gggaaacgct gtttctgccg ccatctccgg ggcgtgccgg ggcctctttc
     7561 ctggcgcgcc cctgctccat attcatgatt catgcaagcg gctggatgcc gggcccatcc
     7621 gcctctggta cctcacgaaa gggccaggga aaggtggagg atcaaaaacc acagcatgcg
     7681 cagtgcgagc agcagcacga caatgatccc gagcacgccg atgatattgc ctgcgagtcc
     7741 gttgcgccga tcgcccatga cgctctggcg gtttgccagc cagagcatcg tggctgccat
     7801 cagggggttg cctaccaccg tcagcgcctg cccgaaaatc atgagattca ccgtggggat
     7861 cttgagcagg agtcccacgc tcgcgatcac aaatcccgta agcagcacgc agatggtgaa
     7921 catgcggggc cagaaatcgt tgagtttgcc cggaaaaccc agtccatcgg cgagggcggt
     7981 gccgccgatc atggcgttga tcaggaacgg gttgagcgca atggggacca atccgataca
     8041 aaagagtgtg aatgcggtcg gaccgagcag cgggtgcagg gtctgagcca gggtcgcaat
     8101 gttgttggcc ggttttccga ggatcaccgt gcccgcggtg atcataatca tcatgctcac
     8161 cagcgtgagc acgcccaccc cggccacgga atcgccaatg ccgcggtcgt aatcgtcggt
     8221 gtcccagccc ttctcacgca ccaggttgcc ctgaaagaac gcgcccgcca ctgaaaacgt
     8281 cgtccccatg agcgaggcga tcagcaccag cggatcattg atggtgccgt ccacgatttt
     8341 cgggacaccc agggaaagct ccttcggaat gccgggcacg agccctttca aaatacccag
     8401 cacgctgggg cgggcgacca gtaggttgac gaggaaggac aagagtatga gtcccaccat
     8461 gaatttcata acgttctcga tcgttttgaa cacggagtgc gcccggaata gaaaaaggat
     8521 cagaacggcg ttgagcaggc ccacggcgcc tatctggaac gcgaggctgt agcgttccgg
     8581 agcaaaggcg ctgacggcga gcgtgacagc gaggttgttt gaaaactgga acgaggcgca
     8641 ggtcaggcac aatacgatcc ccagaagcgc ggcgaaggga cggttgacct cgcgggccag
     8701 cgtggaaaag tggctgctgc cggcggtcac acccacccgg gccgacatcg tcatgtagcc
     8761 gcccatgacc aggccggcca ataccagcag ccagagcagc tcatagccgt aggtggcgcc
     8821 cacgttggcg ctgaccacca ggcttcccgg accaaatacg acacacgccg taatcaatgc
     8881 cggaccgatg gtcttccacc acggcggacg tgcggcctcg acagtgtttt gccggtcatt
     8941 cgacataaga accacccttt cgccattggc gcgcccagtt acacctgcgg acgaacaagt
     9001 ctcccggcgg cataccggct tgaccttcag acggcagcat acgaccccct tgcccgccat
     9061 tctgcccccg ggacatggga atgtcaaaga tctgtgccaa aaacgggccg caaaacgccg
     9121 gttcaggctc tacgtgcgcg tattggagtt gtcaggaggg gattggcacc ctctcccgcc
     9181 gggtcaaaaa aggaagtcaa gggtctattt tcgtctccgg ctggctttct ggatctcgtg
     9241 gaatgttcct ctgctggata tagtcccagt gtcggatata gttgatcgtg aacgggagac
     9301 aagccgcaaa ggctcccaca atcaagccga ttatcagcaa tgggaccgga ctttcccaaa
     9361 gcatctcgaa aggcaagatg tcgagaccaa tccaaagccc cgccccgaac ccaatgacga
     9421 aaagaatgac ggaggcaatg aggatgcccg ccagatccag aagccgatcc tcggtttcct
     9481 ttttcaacgc aatctcctcc acgtgcccta acgcgcgacc tggcacagag ttcagaaccc
     9541 gtttcaggga gctgaaacca gtggtcggcg ctccgcgctc gatccggtct gcgcatccgc
     9601 gaaagagccg gcccttagac cgataggccg ggctccagct cggatttcaa ggccctgacc
     9661 acgtccggag gcagtttgac ggcccggcgg gtctctgcgt tgaccgcgca atggacggta
     9721 cgcatcctgc aagccaggcg gccttcagcc gtatggaatt cgtagtcgag cgtgaaggtc
     9781 cggcttcgca gctcggcgac gcgcaggcgg atgagcgcct gctcccccac aaacagcggg
     9841 cagcggtaat cgctctcggc atgcaccacc gggataaggt aacccgcagt gcggatcaga
     9901 tggccgatat caaggccgtg agagttcagg aaggtctcgt aggcgtcatg cgccagggca
     9961 aaatagcgcc cgaaaaacaa tacgccggcg gcgtcgccat cgcgcagaga gatgcggacc
    10021 ggataggtga acatgcgtcc tccttcggtg gttgaacgcg cgctaagcct gtttcaggaa
    10081 atcccagaaa tttccgccca ggagccccgc gacgtcgcgg tctatctcgc tctgtttcag
    10141 cgtccatccc gtacggagca ggtcggcgta tttgtcggca agcacccgcg cgatgatggc
    10201 gcgtgagtgg ctccacttat acaggacctg atcgagcacg cgggagtcag aatgttgcgg
    10261 caaaacgctg gtgcccagca gttcaaagcg catgcgcgtc atttcctcga tgaggctcgg
    10321 cacattgaga aaccaccagc atccgaagag gaacaaattc ggaaatttgc gcgccgcgat
    10381 ggagagttcg tgctggttct ctcgcgcgag catcgtcacc atgaatttcg cgtccggata
    10441 ggccgcgcac aagcgttcga ccgctccggc gtcgctcttg gccaccccgt cgccggccag
    10501 tttcagggcg gggttcatgg ctcgtttgac gccgatcatc attgcaaacg gcacatgctg
    10561 ctctttcgcg accgggatga tgcactcgtc gatgagccgg gcccgggccg agttttcggg
    10621 atacgcaaaa tcctggggaa gcgagaccgc catatacagg gcgcgggttt tctggagcca
    10681 gtcggtgagg aatcgccgca cctccgccag agcggttccg ctgagcctct cgtcgacgtt
    10741 gtagccccat ccctggaggc gtgcgcaggc gccagtccag tcgttcagga tcggatcgat
    10801 gcgaagggcc gcgtggaagc gcttgtccat ggctgccccg gcctcccaca ccggccgttc
    10861 ggcgtcgtcg aaaggatcgt tggtcatgac gaccgattcg aggttggttc gttcaaaaac
    10921 cgtgttgatg taatcttcga cgctcttccg ggcgaaatag tcgcggtacg cggccagatc
    10981 gcgcgaggcg acatcgagcc caaggcgatc gagcacggtg agcacgccgc ggcaggcttc
    11041 gctgtaggga gaacgctcga taaacagcgt ccgccagatg aaatcggcct gttcccgctt
    11101 cgattgagca aagaatgccg ccggttcaat gcgcgacgcc cggatcacct ccgcgatcag
    11161 gtagtggtac gtgagcagct cgtccatgcc ccagagcaac aggccgccaa atgccggcgc
    11221 atacacgtgg gtgtgaatgt cggtcaccgg cgtgcgatcc accgtctcat gcactgccgc
    11281 gcgcatctca tcgagggtgt cgcgtgaaaa cagggtctcc gaatcggtca tcggaagcgg
    11341 tctcctttga ttgactaaac gtatgccggc cgccggcctt gaaaccgggg cggcagaaac
    11401 ctgcccgtcc gggaggcttt aactcaaccc agcgtccacg ggacacggcg ctcacgatcc
    11461 acaaggcggt tggcctcatc gtcgcccgga aaggtctcgg ttaccggatc ccaggtgagg
    11521 gagcgtttca accagcgcgc gatattgccg agatggcaga ccgtagtcga gcggtggccg
    11581 atctcgacgt ctgcgttggg cgtctggcgt gtacgcacac aatggagcca gtttccgatg
    11641 tgctcgtcgg tgcctcccgt aaacgggatg ggctccagtt ccggcagctc cttatccagg
    11701 gagccgggtt tgatggcgta ccggtcgcga tcgattttga tctcgccctc atctccttca
    11761 aaccatccgc cgcccggggc gccctgatcg tccagtttca cgaccacgcc gttggcgtag
    11821 cgcatgtgca ccggccagcg cgggcccaga tgcacgccgt caaagggcgg ttcctcgtcc
    11881 agcaccggcc agatctcgac cgggcccgac tcgtccatcc ccagcgccca ctgaatctga
    11941 tccagcccgt gcgcacccca gccggtcatc tccccgccgg aatagggccg gaatgaaatc
    12001 cagccccact cgtggtcctg tttgccgccg cggggcatgt agagcttctc atggtagccg
    12061 cggtacggtg tcgggccgca ccacatgtcc caatccaggt agtcgggcgc ctcttcttcg
    12121 ggcaggctgc attcccaggg gctggggtag tttgccgtgt ggaccgtatg cacgtttcca
    12181 atacggcccg cacgaacgag cgcgcagccc acgcggtttt tctgcgagga acgctgctgg
    12241 ctcccgagct ggcagatgcg gccgttggcc cgcgccgcat cgaccatcag tcggccctcc
    12301 cggatggtga gcgtcatcgg cttttcgaca tacacgtctt tccccgcttc catggcgtga
    12361 atcgaatgca gcgcatgcca gtggtcgggc gaggagatga taacggcgtc cagttcgctg
    12421 agggcgagca tttcccggta atccggaaac agcatcgcat cggatatgcc gtacttccgt
    12481 ccca
//
LOCUS       DAOPBQ010000036        12217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_5144_length_12217_cov_3.972209, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000036 DAOPBQ010000000
VERSION     DAOPBQ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12217
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5144_length_12217_cov_3.972209"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PKW60_02295"
     CDS             complement(<1..883)
                     /locus_tag="PKW60_02295"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPJ98292.1"
                     /translation="MDKILIRGGRPLHGSVYVRGAKNAALPLMAAALLSEEPCVLHNV
                     PCLHDVFTMDKILSGMGMRIDFTGRSMTLDASGVNSQEAPYDLVRKMRASFFVLGPLL
                     GRFGKARVSLPGGCAIGTRPVDIHLKGLEALGASISIQEGYVVAEGRLRGADIALDFP
                     SVGATENLLMAACRAKGVTRLTNAAREPEIIDLAILLNELGAQISGAGTDLITVVGVD
                     ALGGAEHAVIPDRIETGTFLLAGVATHGDVTVQNACAEHLTSFLQKLREAGAEVEILG
                     HDVYAFKPVYRPGAKPLP"
     gene            complement(999..1508)
                     /gene="secG"
                     /locus_tag="PKW60_02300"
     CDS             complement(999..1508)
                     /gene="secG"
                     /locus_tag="PKW60_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPJ98293.1"
                     /translation="MYSSVHGWLWWVCILLYVPACLGLIAVVLLQKGKGVGFAGAFGL
                     GGGGADTVFGPRMSKSLPVKLTYVAAAMFLLLALAMSLLSGRITKGQAPELVKPETID
                     MSGYEEAGIGAAPGAPAAASEAVETGTTAPAAAEKSAPAEPAAAVVDESPAETPVGGS
                     GEASPPPAQ"
     gene            complement(1592..2356)
                     /gene="tpiA"
                     /locus_tag="PKW60_02305"
     CDS             complement(1592..2356)
                     /gene="tpiA"
                     /locus_tag="PKW60_02305"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPJ98294.1"
                     /translation="MRKPLVAGNWKMHKLVGESLALVNALKPLVKDIDGVDVVVCPVF
                     TSLYPVAEALKGSAIQLGAQNCFTRESGAYTGEVSPQMLKDAGCTWTIIGHSERRQYF
                     NESDEFLNEKLRFALASGLKVMFCIGETLEEREGGRMNDVLVRQVTNGLMGLSESDFD
                     NTSIAYEPVWAIGTGVTATPEQAEEAHAFVRSLVRDQFGEAVADKVRIQYGGSVKPDN
                     VAALMAKPNVDGSLVGGASLNAEGFAELVKKSLKTS"
     gene            complement(2475..5687)
                     /locus_tag="PKW60_02310"
     CDS             complement(2475..5687)
                     /locus_tag="PKW60_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPJ98295.1"
                     /translation="MKSGLTDMALRRPITVTMLLLTVMGLGAIAWQRIPLEFIHRMDF
                     PMMSCSIPYPNATPEQVEAEIAIPAEGEFRTLSSLKRITTTSNSDGCSIRLLFDSDTK
                     MSIATSEVRDRMERLRLKLPSGVDDIYLFRFSSQSLPILLIGLGREGDGEELAHRIRT
                     LLQPKLLRVHGVAEVQVFSKPEQEVLIEFDQDALRAHHVAMYEVVAQLQRSSLNITVG
                     EMKDGPTKFYVRALGEFTHPDEISNLPLAQNGLRVRDIANVGFAAREFQREFTVDGKG
                     GAFILIRKEAEANAIATCKRVHEALEKARQEPDFKGLDIYVFFDQGQLIRQSLDSLIE
                     AGKSGALLAIVVLFLFLLRIRPTLVVALAIPASVVVSLVVMYFIGMTLNIVTMCALII
                     ALGMLVDNAIVVIENIYRYRQLGLDPLESARRGASEVGMAITASTLTTIVVFASLLFM
                     QSGEMGQHMRQFSLPITVSLVASLVIALTVIPLASSRMRELSNLSEHPVLRRLAACIE
                     RVPGGKSIRLIHLLRRVHPLQSLIDGYIGTLHWVMRWRLASIVLLFLFGVITAVIPFR
                     HVGMQGMPEIDTREVRIAVNFDQNFDMDMARAVMDQLLTALNGQRDELGIENIFVNYG
                     PEGGEISCFLYDPDEYPEGRALPFSTEQVMDILWQRFSAPGTGTRLPGAELGFSIAQS
                     EGATRTIRVRMRGDHAAILAEKSQHFSRLMEQIPDISEVQTDAERDRQEVQISIDEAL
                     AKRFGINPMVVARTVDFALRGTRLPYMKREGREIAVWAQFREEDRKRKTSLDNVSLLT
                     PRGELVPLNRVVRFEKAYSPKQITRINSKNVATITANVSGDNLVQIKKHLEDLAASFP
                     LPRGYSIELGDELLELAQDRANFITGIILAIVMIYLVMGALFESFILPLSILTTIPVA
                     FMGVWWTMFLTRTSMDTVAYIGMFLMVGVIVNNGIVIVDHINQLRLGGSERLQATIQA
                     GRDRFRPVMMTALTTILGCVPLAMGGGVGGEIAFHSLGKALIGGLTSGTILTLFVVPL
                     FYSLIDDFRDWMMGYFAGLAALGSRRLPVPHPGETTQAGL"
     gene            complement(5684..7039)
                     /locus_tag="PKW60_02315"
     CDS             complement(5684..7039)
                     /locus_tag="PKW60_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPJ98296.1"
                     /translation="MEAPPGTRLEQTEQQAIQVEKIVRSYPLVDTVSVEVGSEKSKAE
                     SERGENVAEFTVTLKDPDENAQYQDEIIEQIRHEIIARTGNEITFTLPALFSFKFAVE
                     LQIQGDDIDTLRGVGQEILAAIQDVPGLKDAELNMKRGYPEVIIGLDREMLAAKGLTP
                     DQVALRLRNEVQGDAPTEFNRAGEKVDIRVRTDRAGLSSLRDLRQVSVIEGTPPIPLE
                     TVARIDVKEGPSEVRRIDQRRVALVTANVEGRDLGAVSRDIEARVSRIRMPDDFDWTL
                     GGQNRELATSYQSLQFALLLAIFLVYVVMACQFESILHPALVLFTVPLAFIGVVYVLY
                     FLGMNISIMVFIGGIILAGIVVNNAIVLVDYVNQLRARGMAKREAVVQAGLVRLRPIL
                     MTTLTTVLGLIPMALSSGEGAEIRRPMAITVMAGLSSATLLTLMIIPMAYHVFGGRDK
                     T"
     gene            complement(6943..9243)
                     /locus_tag="PKW60_02320"
     CDS             complement(6943..9243)
                     /locus_tag="PKW60_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPJ98297.1"
                     /translation="MSEAQSPPIQQHRLAIRRPVTTAMVFLTLVVFGWKSYEGLSINL
                     MPDISYPTLTVRTEYEGAAPEDVEKLLTRPLEETLAIVPGMVQISSVSSPGLSEIVLE
                     FTWDTDMNTAQQDVRDRLDLFDPPQEVTQKPVILRYDPTLDPIMRVAIVDTSEDTARQ
                     QERLTQIRESAERHIKSDLEGELGIAQVLVKGGRKEEIQVLVDSRKLKNLGLNLDSIS
                     NALAQQNINLSGGRLKEGKTEYLVRTVNEYKSVSEIGDTLIVPPAGIPIALSQVAEVI
                     QGEKDRDTVIRLNGGEAVELEIYKWGDANTVQVCNKLKDLFGFERKLSFWELAAERLD
                     RLRNAGEENEETRRSRDLRRTLLSRLPDPDRSQFVLVSDQSRFIVASIEEVQNTAIQG
                     GLLALIILAFFLKQLRSTLIIGVSIPISIIATFVPMYMREVSLNIMSLGGLALGVGML
                     VDNSIVVLESIFRCSEEGDDTINAADRGTNEVAGAVTASTLTTVAVFLPLAFVEGIAG
                     QLFGDLALTVTFSLLASLLVALYLIPMVAARPPLSLLADRDVVWMLRAYHDGRATGRG
                     RLASMAGLAPRGWNYVMAWLREAAHHDFRPLTAAWKGRARRGALGNFLAAVKGALLAP
                     CLVFLFGMRVFLMSIAALLISVVFPVSLLLVAVFWVIRQAVSLLLWVPFWLFETAFNG
                     FRKAYETILTSSLNYSILVLIAMLGLSAHAGWLIPKLGTELIPPLKQENSGSAWKPLQ
                     APGLNRPSSRRSRWRKLSAAIRSSTP"
     gene            complement(9337..10626)
                     /locus_tag="PKW60_02325"
     CDS             complement(9337..10626)
                     /locus_tag="PKW60_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPJ98298.1"
                     /translation="MYSYLRILGVAAALWAVCGCGGTTGANTDAKAAPEFTIDEIVIP
                     VEVESPSRGDISAYFETTTRVEAEKRVDVTSEALGTCVAVFADEGDTVKEGQVLAELD
                     KAEAEASLRQAMVSESERKTAYERARQAFDAGILAEQDRDASQFAYENAVATRRIQEV
                     RLANLTIRAPLSGVVTHRHIQVGMLVSTGTPAFSVVDPSSYQLVISPPEKNLMQLREG
                     QAAKFTVDAIAGEEFEARITRINPSVDPTSGTVKVVLSLSRATQARLREAAFVRVRLV
                     METHENVLLVPKDTVIEENARTYVFVARPRAMEDSADGGESSALEQEADSDAAAAAPE
                     AMETEQWIAERTEVEVGLEDSDASEIVSGLNESDLVVTLGQQTLKPGTRISITTAERE
                     LARSAGLGATEALKAAEEAKKKKEAPQASRQRRHRLP"
     gene            complement(10873..11223)
                     /locus_tag="PKW60_02330"
     CDS             complement(10873..11223)
                     /locus_tag="PKW60_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98299.1"
                     /translation="MKARSKSEWKMAIREEAKRTVKGPRSDVHTSGGYSKQIEAGLNE
                     LRTKMSEGGDGKAKRIKSELTFLLNNYAPQYDMRIEHLIDEWRRSGDPQYDPGIRMKF
                     RNARRAHMRDSGRL"
     gene            11601..>12217
                     /locus_tag="PKW60_02335"
     CDS             11601..>12217
                     /locus_tag="PKW60_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98300.1"
                     /translation="MSDELPDNSSQLPADLPEQGAQPPETAPEMVQSPNAGDAGQSNL
                     DTLIERLRLRDSPAGGGEDTSQAPSAAGAGLEPSNSSEMDEILASIAGEQDEQSIEVA
                     AGAPRAVAPVAPPFDEFTPEASSELDLAPGELPDPEVPVAGLDDLDTLIARVDDSLRD
                     KVESRGPNPEAEPGSPPAKKESGKTGDMDAVIAALTGGGAGADGDV"
BASE COUNT         2404 a         3938 c         3405 g         2470 t
ORIGIN      
        1 ggggcagcgg tttcgccccc gggcggtaaa cgggtttaaa agcgtagaca tcatgcccca
       61 gaatctcaac ttccgccccc gcctcacgca gcttctgcag aaaactcgtc aggtgctccg
      121 cgcaggcgtt ttgaaccgtg acgtcgccat gtgtggccac tcccgccagc agaaaggtgc
      181 ccgtttcgat gcggtcgggg ataaccgcat gttccgcgcc gcccagggca tccactccca
      241 ccacagtaat gagatctgtc cctgcgccgg aaatttgcgc tcccaattcg ttcagaagga
      301 tggcgagatc gatgatctcg ggttcgcgcg cggcattcgt caagcgtgtg acccctttcg
      361 cgcggcaagc ggccatgagg aggttttccg ttgcccctac gctggggaag tccagcgcga
      421 tgtcggcgcc gcgcaggcgg ccttccgcga cgacgtagcc ttcctggatg cttatggaag
      481 ctcccagggc ttccagccct ttcagatgga tgtccaccgg gcgggttccg atagcgcagc
      541 cccccggcag cgacacccgc gccttcccaa agcgccccag aagagggccc agcacgaaaa
      601 acgacgcgcg catcttccgc accaggtcgt acggcgcttc ctgggaattg acgccggagg
      661 cgtccagggt catcgagcgc cccgtgaaat ctatgcgcat gcccatcccg gacagaatct
      721 tgtccatagt gaacacgtcg tgaaggcacg gtacgttgtg aagtacgcac ggttcctcgg
      781 acagcagggc cgcggccatc aacggcaatg ccgcgttttt ggcccctcga acgtacaccg
      841 aaccgtggag tggccggcct ccgcgaatca ggattttgtc catgcctgga gtgacgctcc
      901 tttactctga caggcgtttg ttccgcaata ctaaccccgc aaaagatacc ggcccagagc
      961 cggggaaggc tctgggccgg gtcgcgtttc catatgcctt actgcgcagg cgggggtgaa
     1021 gcctccccgg aaccgccaac aggcgtctcc gcaggagatt catccacaac ggccgctgcg
     1081 ggttccgctg gagcgctttt ttcggccgcc gccggggcgg ttgtgccggt ctcgacagct
     1141 tcggatgccg ccgcaggcgc accgggcgcg gccccgattc cggcttcttc atagccgctc
     1201 atgtcgatgg tctcgggttt caccagttcg ggtgcctgac ccttcgtgat tcgacccgaa
     1261 aggagcgaca tcgccagcgc cagcagcaga aacatcgccg ccgccacgta cgtaagcttc
     1321 accgggaggc ttttgctcat ccgcgggcca aataccgtat ctgcccctcc accgcccagg
     1381 ccgaacgcgc cggcaaaccc aacgcccttg cccttctgga gcagaacgac ggctatcaaa
     1441 cccagacatg cggggacata caggagtatg cacacccacc acagccagcc atgaaccgag
     1501 ctatacatcc gtgcaaattt cctctcaggc tgcccgcgtt gccgccctgt ccgttcggag
     1561 caggccggcc cggggccgtc ccttgtctag gttagctggt tttcagcgac ttcttcacca
     1621 gctccgcaaa accctcggcg ttcaacgacg ccccgcccac gagcgagccg tccacattcg
     1681 gcttcgccat cagtgccgcc acattatccg gctttacgct gccgccgtat tgtatccgaa
     1741 ccttatccgc cacggcctcg ccaaactggt cccggacgag gcttcgcaca aaggcgtgcg
     1801 cctcttcggc ctgctccggg gtggcggtga cgcccgtgcc aatggcccag acgggttcgt
     1861 atgcgatgga ggtattgtca aagtcggatt ccgacagccc catcaggccg ttggtgacct
     1921 ggcgaaccag cacgtcgttc atccggccgc cctcacgttc ttcgagggtc tcgccaatac
     1981 agaacatgac cttcaggccg ctggccagcg caaagcgcag cttttcattc agaaactcgt
     2041 cggattcgtt gaaatactgg cgccgttcgc tgtgcccgat gatggtccac gtgcatcctg
     2101 cgtctttcaa catctgcgga gatacttcac ccgtgtaggc gccgctttcc cgggtgaagc
     2161 agttttgtgc tccgagctgg atcgcggagc ctttcaatgc ctccgctacc gggtacaatg
     2221 atgtaaacac agggcataca acgacatcga ctccgtcaat gtcttttaca agcggtttca
     2281 gcgcgtttac caaggccaac gattcgccca ccagcttgtg cattttccag ttacccgcga
     2341 ccagtggttt tctcacaaca tccccttcca ttttctggcc cgccaagcag cggaatcagc
     2401 aactatccga aacagctcac aagcgtatca cacgaccatg cagggaaaca aattcgcggc
     2461 agcagcgcgc cgggctacag gcccgcctgc gtggtctccc cgggatgagg cacggggagc
     2521 cggcggctgc ccagcgccgc caaaccggca aaatagccca tcatccagtc gcggaaatcg
     2581 tcgatgagag aataaaacag cgggacaaca aacagcgtca ggatcgtccc cgacgtcagt
     2641 ccgccgatca gagccttacc gagactgtga aacgcgatct cgcctccaac cccgcctccc
     2701 atggccaggg gcacgcagcc cagaatagtc gtcagggcgg tcatcatcac cgggcggaac
     2761 cggtcccggc cggcctggat cgtcgcctgt aggcgttccg aaccgccgag gcgcaactga
     2821 ttgatatggt caacaatgac gatgccgttg tttacaatca cgcccaccat caagaacatc
     2881 ccgatgtagg cgaccgtgtc catcgacgtt ctcgtcagga acatcgtcca ccagaccccc
     2941 atgaaagcca ccggtattgt cgtcaaaatc gacagcggca gaataaatga ctcgaacagc
     3001 gcgcccatca cgaggtaaat catgacaatg gccaggatga tccccgtgat gaaattcgcc
     3061 cgatcctgcg cgagctccag caattcgtct cccagctcga tggaatatcc ccgcgggagc
     3121 ggaaaacttg ccgccagatc ctccagatgc ttcttgatct ggaccagatt atccccactc
     3181 acgtttgcgg taatggttgc cacgtttttg gagttgatcc gcgtaatctg tttcgggctg
     3241 taggcctttt cgaagcggac cacccggttc agcggcacca gttcccctct cggcgtcagc
     3301 agggagacat tgtcgaggct ggtcttgcgc tttcggtcct cctcccggaa ttgtgcccag
     3361 acagcaatct cacgcccttc ccgtttcata taggggagcc gggtgccgcg caaggcaaaa
     3421 tctacggttc gtgccaccac catgggattg atgccaaacc gtttcgccaa tgcctcatcg
     3481 atgcttatct gaacttcctg ccggtcgcgc tccgcgtccg tctgcacttc acttatatcc
     3541 ggtatttgct ccatgagcct gctgaaatgc tgggattttt ccgcaagaat cgctgcatga
     3601 tctccccgca tccgaacccg gatcgtccgc gtcgccccct ccgattgcgc gattgagaaa
     3661 cccagctcgg cgccgggcag ccgcgtgccg gttcccggcg cggagaagcg ttgccagagg
     3721 atgtccatga cttgttccgt ggaaaatggc agagccctgc cctccgggta ctcgtccggg
     3781 tcgtacaaaa agcagctgat ttctccgccc tcgggcccgt agttcacgaa tatgttctcg
     3841 atgcccagct cgtctcgctg gccgttgagc gcagtcagca gctggtccat gacggccctg
     3901 gccatatcca tatcgaaatt ctggtcgaaa ttgactgcga tgcgaacctc tcgcgtgtca
     3961 atctcaggca tgccctgcat gcccacgtgc ctgaacggga tcaccgcggt gatgacgcca
     4021 aacagaaaga ggaggactat cgaagccagt cgccaccgca ttacccagtg aagggttcca
     4081 atgtatccgt caatcagact ctgcaatgga tggacacgcc tgagcagatg aatcagccgg
     4141 atcgatttac ctccgggtac ccgctcgata catgcggcca gccggcgcag aactgggtgc
     4201 tccgacaggt tggacagctc tctcatgcgg ctgctggcca gaggaatgac cgtcagtgca
     4261 atcaccaggc tcgctacgag agagacggtg atcggcaggg agaattgccg catgtgctgg
     4321 cccatctcgc cactctgcat aaacagcagc gatgcaaaca cgacgatggt cgtcagggtc
     4381 gacgccgtaa tcgccatccc gacttcagag gcgccgcgcc gggcgctttc cagcgggtcc
     4441 agtccaagct gccggtaccg gtagatattt tcgatgacta caatcgcgtt gtccaccagc
     4501 atgccgagcg cgatgatgag cgcgcacatt gtgacgatat tcaaagtcat cccgatgaag
     4561 tacatcacca ccaggctgac taccaccgac gccggaatgg ccagtgcaac cacgagcgtc
     4621 gggcgaatcc gcaacagaaa caggaacaga accacgatgg cgagcagcgc acctgatttt
     4681 cccgcctcaa tcaggctgtc cagcgattga cggatcaatt ggccctgatc gaaaaacacg
     4741 tagatatcca gacctttgaa atcgggctcc tgccgtgcct tttccaaggc ctcgtgcacc
     4801 cgtttgcacg tcgcaatggc attggcttcc gcttctttgc ggatcagaat gaatgcgccg
     4861 cccttgccgt ccaccgtgaa ctcgcgctga aattcccgcg ccgcaaaccc gacattcgca
     4921 atgtctctca cccggagacc gttctgggcg agcggcaggt tggatatctc gtccggatgc
     4981 gtgaattcgc cgagcgcccg tacgtagaac ttcgtcggac cgtccttcat ctcgcccacg
     5041 gtgatgttca ggctgctgcg ctggagttgc gccactactt cgtacatcgc cacgtggtgc
     5101 gcccggagcg cgtcctgatc gaattcaatc agcacttcct gttccggctt gctgaagacc
     5161 tggacttccg ccaccccgtg cacccggagc agcttcggct gaagcagggt ccggatccgg
     5221 tgtgccagtt cttcgccatc gccctcccgg cccagtccga tcaacaggat cggcagcgac
     5281 tgcgaactga atcgaaacag atagatgtcg tcgacgccgg atggcagttt gaggcgcagc
     5341 cgttccatac gatcccgcac ctccgaggtc gcgatcgaca tcttggtgtc cgagtcaaac
     5401 agcagacgga tgctgcatcc gtcgctgttg gaggtggtcg tgatgcgctt caggctcgac
     5461 agcgtacgga actccccttc cgctgggatc gcgatctccg cttcgacctg ctccggcgtg
     5521 gcgttggggt aggggatcga gcaactcatc atcgggaagt ccatccgatg aatgaattcc
     5581 agcgggattc tctgccacgc gatggctccg agccccatca ccgtgagcag cagcatcgtg
     5641 acggtgatcg gccgccggag cgccatatcc gtcaacccgg atttcatgtc ttgtcccggc
     5701 cgccaaacac gtggtaggcc atcggaataa tcatcagcgt aagcagcgtg gccgacgaga
     5761 gccccgccat gaccgtaatg gccatcggac gccggatttc tgcgccctcg cctgatgata
     5821 acgccatcgg aatcaatccc agaaccgtcg tcagggtggt catcaggatc gggcgcagcc
     5881 ggacgaggcc tgcctgcacc accgcttccc gtttggccat gccccgtgcc cgcagctgat
     5941 tcacgtaatc cacgagcaca atggcgttat tgacaacgat gccggcaaga atgatcccgc
     6001 cgatgaacac cataatgctg atgttcatcc ccagaaaata caggacgtat accaccccga
     6061 tgaatgccag cggcaccgta aacagcacca gcgccggatg cagaatggat tcgaactggc
     6121 acgccatgac cacatagacc aggaagatgg ccagcagcag cgcaaactga aggctttgat
     6181 acgaggttgc caattcgcgg ttctgcccgc cgagggtcca gtcaaaatcg tccggcatac
     6241 ggatccggga tacccgtgcc tcgatatccc gtgataccgc gcccagatcg cgtccttcca
     6301 cattcgccgt gaccagggcc acccggcgct gatcgatgcg ccgaacttcg ctgggccctt
     6361 ctttcacgtc gatgcgtgcc acggtctcga gcgggatggg cggcgtacct tcgatcacgg
     6421 atacctggcg caggtcgcgc aggctgctca atcctgcccg gtccgtccga acccggatgt
     6481 cgactttttc gccggcccgg ttgaattccg tcggcgcatc gccctgcacc tcattccgca
     6541 gacgcagcgc cacctgatcg ggcgtcaggc ctttcgcggc cagcatttca cggtccaatc
     6601 caatgatcac ctccggatag ccgcgtttca tgttcaattc cgcgtctttc aaccccggga
     6661 cgtcttggat cgctgccaga atttcctgcc ccacgccgcg cagggtgtca atgtcgtcgc
     6721 cttgaatctg aagttcgacg gcgaatttga agctgaataa ggccggcagt gtaaacgtga
     6781 tttcattgcc ggtacgggcg atgatctcgt gccggatttg ttcgatgatc tcatcttgat
     6841 actgggcgtt ctcatccgga tctttcaagg tgaccgtgaa ctccgccacg ttttcgccgc
     6901 gttcgctttc tgctttggat ttttccgaac ccacttccac gcttacggtg tcgacgagcg
     6961 gatagctgcg gacaattttc tccacctgga tcgcctgctg ctcggtctgt tcaagccggg
     7021 tgcctggagg ggcttccatg cggatcccga attctcctgt ttcagcggcg gtatcagttc
     7081 cgtccccagt ttcggtatca accacccggc atgtgcagac aggcccagca tcgcaatcag
     7141 caccaggatg ctgtaattca ggctcgaggt aaggatggtc tcgtatgctt tgcgaaatcc
     7201 gttgaatgcc gtctcgaaca gccagaacgg gacccacagg agcagcgaca ccgcctggcg
     7261 tatcacccag aataccgcga ccagaagcag cgagacgggg aaaacaaccg atatcagcag
     7321 cgccgcgatc gacatcagga acacgcgcat tccaaagagg aacacgagac agggcgccag
     7381 tagtgcgcct ttcacggcgg cgagaaaatt ccccaaagca ccccgccggg cgcgcccctt
     7441 ccaggcggcc gtcagcgggc ggaaatcatg atgcgccgcc tcgcgcagcc acgccattac
     7501 gtagttccac ccgcgcggcg ccaggcccgc catcgacgct aagcggcctc tgcccgttgc
     7561 ccgcccgtcg tgataggccc ggagcatcca taccacatcg cggtccgcca gcaggctcag
     7621 cggcgggcgc gcggccacca tcgggatcag atacagcgcg accagcagtg acgccaggag
     7681 cgaaaaggtc accgtcaggg ccaggtcgcc aaacagctgg cccgctatgc cctccacgaa
     7741 cgccagagga agaaatacgg ccaccgtggt cagggtggat gcggtgaccg cgccggccac
     7801 ctcgttggtg ccccgatccg cggcgttgat cgtgtcgtcg ccctcctccg agcaccggaa
     7861 aatgctctcc agcaccacga tcgagttgtc tacgagcatg ccgacgccca gcgccagacc
     7921 gcccagggac atgatattca gcgaaacctc ccgcatgtac atcggcacaa acgtcgctat
     7981 gatcgaaatg ggaatggaaa ccccgatgat cagggtgctc cggagctgtt ttaaaaagaa
     8041 agccagaata atcaatgcca gcagaccgcc ctggatggcg gtgttctgga cctcttcgat
     8101 cgaagccacg atgaaacggg actggtcgct gaccagcacg aactggctgc ggtccgggtc
     8161 tggaagccgc gaaaggagtg tccggcgcag atcccggctg cggcgcgttt cttcattctc
     8221 ttccccggcg ttccgcagcc ggtcaagacg ctctgccgcc aattcccaga aactgagttt
     8281 ccgctcaaat ccaaacaggt ctttgagttt gttgcacacc tgtacggtgt tggcgtcgcc
     8341 ccatttgtag atctcaagtt cgaccgcttc gccgccgttc aggcgaatca cggtatcgcg
     8401 gtctttctct ccctggatga cttccgccac ttgagacagg gctatcggaa tgcccgcggg
     8461 cggcacgatc aaggtgtcgc cgatttcgga taccgacttg tattcattga ccgtccgcac
     8521 caggtactcg gtcttgcctt ctttgagccg tccacccgag aggttgatgt tctgctgagc
     8581 gagcgcgttg ctgatgctgt ccagattcaa gcccaggttt ttcagtttgc gcgagtcaac
     8641 cagaacctgg atctcttctt tgcgcccgcc tttgaccagc acctgcgcga tgcccagttc
     8701 cccttcgaga tcgctcttga tgtggcgttc cgccgactcg cgaatctggg tcaggcgctc
     8761 ctgttgccgc gccgtatctt ccgatgtatc cacaatggcc acgcgcatga tcgggtcgag
     8821 cgtcgggtcg tagcgcagaa tgaccggttt ctgggtgact tcctgcggcg ggtcgaacag
     8881 gtccaggcga tcgcgcacgt cctgctgcgc ggtattcatg tccgtatccc acgtgaattc
     8941 cagcacaatt tcggataaac ccggcgagga tacgctgctg atctgcacca ttcccggcac
     9001 gatcgccagc gtctcctcca gcgggcgcgt cagcagcttt tccacgtctt ccggggcggc
     9061 accttcatat tccgtgcgca cggtaagcgt gggataggag atgtccggca tgaggttgat
     9121 cgaaaggcct tcgtaggatt tccagccgaa caccaccagc gtcaaaaaaa ccatcgccgt
     9181 ggttactgga cggcggatgg ccaggcgatg ctgttgtatg gggggagact gcgcttcact
     9241 catacgtata cttgacactt cctgagatgg ccacaggccc ggaggcctgt acagaacgta
     9301 ccgtcagccg cgctcgcaca acagagcatg aggcggctag ggcaaccgat ggcgccgctg
     9361 ccgggatgcc tggggggcct cttttttctt tttggcctct tcagccgctt tcagcgcttc
     9421 cgttgcaccc aatcctgcgc tgcgagccag ctctctctct gccgtcgtga tgctgatgcg
     9481 cgtccccggc ttcagcgtct gctgccccag cgtcactacc aggtcgcttt catttaaccc
     9541 ggagacaatt tccgatgcat cgctgtcttc gaggcccacc tccacctcgg tccgctccgc
     9601 aatccactgc tctgtttcca tcgcctccgg cgccgcggcc gcggcatcgg agtcagcttc
     9661 ctgctcgagt gccgaagact cgcccccgtc cgcgctgtct tccatcgctc tgggacgcgc
     9721 cacaaaaaca tacgtgcgcg cattctcctc aatcaccgta tctttgggaa ccaggagcac
     9781 gttttcgtgg gtttccatca ccagacggac tcgcacaaac gccgcttccc gcaagcgcgc
     9841 ctgggttgcg cggctcaggc tcaacaccac cttcaccgtg ccgctcgtgg ggtctacgct
     9901 cgggttaatg cgtgtgatgc gcgcctcgaa ttcttctccg gcaatcgcgt ccactgtgaa
     9961 tttggcggcc tgcccttccc ggagctgcat caagttcttt tcaggcgggc tgatgacaag
    10021 ctggtaggac gacgggtcta cgacggaaaa tgccggcgtt cccgtcgaca ccagcattcc
    10081 cacctggatg tgccggtggg ttacgacgcc gctgaggggc gcacgaatcg tcaaattggc
    10141 cagccggacc tcctgaatgc ggcgcgttgc cactgcattt tcgtatgcaa actgggacgc
    10201 gtcccggtcc tgttccgcca atattcccgc atcaaaggcc tgccgggccc gttcataggc
    10261 cgttttccgc tcactttctg acaccatagc ctggcgcaac gaggcctccg cttcggcttt
    10321 gtccagttcc gccagtacct gcccttcctt gaccgtgtct ccctcatcgg caaaaacggc
    10381 cacgcaggtc cccagagctt ccgaagtcac atcgacgcgt ttctcggcct cgacccgcgt
    10441 cgtagtctcg aaataggctg aaatatcgcc ccggctcggg ctctccacct ccacggggat
    10501 gacgatctcg tcgatggtga attccggtgc ggctttggca tccgtgttcg cacccgtggt
    10561 accgccgcat ccgcagactg cccacaaagc ggccgcgacg cccagaattc tcaaatagct
    10621 gtacatcgta cctccacgcg ggatggaaat cgcgttccag ataatccggt tcgtgtgcaa
    10681 atcggcgcat ggtaaagggt ttcgcatgga aacttcaagg ccacatgcca cgccacgccc
    10741 gtcccatcca gcggcctgaa ttacaacggt ttgaccccgc agacgggtgc gaagtttcat
    10801 ttgcgccaaa attggcggag ggagaagccg gggagccggg gccgctgggc tgagctgcgg
    10861 tccgaagggc gcctacagcc gtccgctgtc tctcatgtgt gcccggcgcg cattccggaa
    10921 tttcatgcgg attcccgggt catattgcgg gtcaccgctt cgccgccact cgtcgatgag
    10981 atgctcgatg cgcatatcat attggggtgc ataattgttc aggaggaaag tcaattcgct
    11041 tttgatgcgt tttgctttcc catccccgcc ctcgctcatt ttcgttcgca gctcgttcag
    11101 gccggcctca atttgcttcg aatagcctcc gctggtgtgc acatcgctcc ggggcccctt
    11161 taccgtgcgt ttggcctctt cccggattgc cattttccat tcagactttg aacgggcttt
    11221 catgaccgag tcctcctcgc agccggggag ccacgacgtt gccaattcga ttgagaaagt
    11281 atggtctatg tgtcacgcca agatcaattt tcacactgtc aacgatcgca gtttgcgcca
    11341 gagttaagct gcaaactctc tgaaaggcac aaaatcttgc cggtcaccgg cgctccgctc
    11401 agtcaatctt tcacttcatt gcgggcgttg aggtcggcaa aaaattcctg tttgtgttgc
    11461 tacgtgcccg tctccgtgct acactaccag ttagtttatt gacattcagt caggatgcgc
    11521 agaaatcctg tgcgaagcag gtatgcggcg cgaataaacg cgcccttgaa ccggggagtt
    11581 cggcgtcaaa aggaagacca gtgtcagacg agcttccaga caattccagc caacttccgg
    11641 cggaccttcc agaacagggc gcgcagcccc cggaaacggc gccggagatg gtgcaatcgc
    11701 ccaacgccgg cgacgccggc caatcaaacc tggacaccct gatcgaacgc ctgcgactcc
    11761 gggattcgcc cgccggcggg ggagaggaca ccagccaggc gccgtctgcg gcaggtgccg
    11821 gacttgaacc atccaattcg tcagagatgg acgaaatcct cgcgtcgatt gcaggcgaac
    11881 aggacgagca aagcatcgag gtggccgccg gcgcacctcg tgcagttgcg ccagtcgctc
    11941 ccccgttcga cgagttcacc ccggaggcga gttccgaact ggatctcgcg ccgggcgagc
    12001 tgcccgatcc cgaagtaccg gttgcgggcc tggatgacct tgataccctg attgcgcggg
    12061 tcgatgacag ccttcgcgac aaggtggaaa gccgtggccc gaatccagag gctgaaccag
    12121 gttcaccacc tgcaaaaaaa gagtccggga aaaccggcga tatggatgcc gttattgccg
    12181 cgctgaccgg gggaggcgct ggcgcggacg gggatgt
//
LOCUS       DAOPBQ010000037        12031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_5250_length_12031_cov_3.983634, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000037 DAOPBQ010000000
VERSION     DAOPBQ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12031
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5250_length_12031_cov_3.983634"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="PKW60_02340"
     CDS             <1..1857
                     /locus_tag="PKW60_02340"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPJ98301.1"
                     /translation="AFAEQLPDRTGELREPCRTSGRGGQYLIVHTLSSRYPLKKANDE
                     CQALLERWAEPAALMARMKGGEPNLQYIDLAWEYLIKNHAHDSICGCSLDQVHRDMRY
                     RFDQCRMLADGLVRRIVADIGGASDSPEALANTVLHNPLPHERHGVFELALPFPADYA
                     PKYVDGLVTGERVNRFRLLTADGTPIPFQMARIEHGVLSKRIRNNGRYTNFEGDVYHV
                     AAELTLPACGYTGIKIEPTEAATTRNTGTLLTGMLTADNGHIELSFQSDGTASLTEKA
                     SGKTYGPLFMYEDTGDWGDGWTRGPLVKDIVFKTPGNNVSVAVDEDGPLRTVFRIERE
                     LHLPRMVDKATRWRADDRIALRITDRVYIDKDTPYVRVRTRVENTVEDHRLRVLFPTP
                     IATDTSFAETPFAIVERVIDPPPETAEWHERVNPETAFTGLFGIQDAEGGLAVLCPRG
                     LHEYEVTRTSQRVLALTLFRSTYQTIGTPGEPDGQLLEPMDFEYLLYPFAAPFNPVAA
                     LRTVQEAQTPIRSHQADALPGTFSFCRVESARAVVTALKPAADRNGGIVRLWNPGTTP
                     AEASVVFAQSLKTAHTCNLNEEAQAELALGDGGKQFAVEIPPHALSTVRFTC"
     gene            1923..2126
                     /locus_tag="PKW60_02345"
     CDS             1923..2126
                     /locus_tag="PKW60_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HPJ98302.1"
                     /translation="MISGLGLIVVGGAIWLLAKSGLPLGRLPGDIHVEGRHSSFHLPL
                     VTCLVISAVLTLLLNLALWLFRK"
     gene            complement(2189..3748)
                     /locus_tag="PKW60_02350"
     CDS             complement(2189..3748)
                     /locus_tag="PKW60_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98303.1"
                     /translation="MKTFPKLSGCLGMSRRGFLASGCAACLGATMCTPAKSAAAAART
                     AEKMRIRTIYSLHAEVQPGPDWPNVGYDFRPQMERINSELAAAFPKYEFLPTLATGPE
                     QAQQILEEDASGKIDGYIVYQMNCWNQVVQTIATSGKPVLYADFQYAGSGGFLVYTAA
                     FLRNSTPNVGFVASSDMKDQIAAVRCFDVVRKGGSPADFAAATARARIKSTPKPGRLT
                     CKGDPIDFLSPEDCLARMKASRILAVRDQEAGDGGEFMGIPITRVAFAEVNEAWANAD
                     RDEARAIADRWEKDAAVIEGVSRETLETSAAMYLGQKAVLKKYGANAITINCLGGFYG
                     GHIHAYPCLGFHELNNEGLIGACECDIPSTATMVTFSALTQGRPGYISDPVIDTAKGE
                     IIYAHCVASNRPFGPQGPANPFEILTHSEDRQGASVRSIFPLGYMTTSLELSSDRKEV
                     LFHQGKAVENDRNDRACRTKLGVKPVGDIEKLFSMWDQWGWHRVTFYGDLKEPVFALA
                     DAAGWKVVEEA"
     gene            complement(3971..6607)
                     /locus_tag="PKW60_02355"
     CDS             complement(3971..6607)
                     /locus_tag="PKW60_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HPJ98304.1"
                     /translation="MKTWLVPSFILAFVSMGAVADDALLGDLLPRPQKVQILPGQCLL
                     NTDEMHIMLTAPGDEQIKRLTDRLIESLIRLDLNITPAAASGETYLLYVSFFGTPAPF
                     APTTVLPAAARDQGYALTVTPSSVLIGAASEKGLFYGLMTLEQLLRSAQLRERTALPC
                     MRILDWPALTMRGYSEDYGRDQLPTMDEHKRAIRLLARFKIDTYLWFIEPDHFVYGFD
                     PELGQEYDRFRFEEIRELVAYAREYYIDVIPTVELLGHMEMTLREPRYRHLAEMPEGG
                     GDLCATSDEAFDLVRNMVTEIAPAFDGQYFHCGLDESYAIGKGRSADAVREKGLERVF
                     ADYYTKMNELVKSQGKTMMMYADIILNHPGILDLLPGDIVPMFWDYAPRDVYPGLTTL
                     KESGMTFTALSGLWDWNNLYPIYPPAFQNMETLAAQAANEGALGHFVSSWGDGYKGAA
                     GTNLSELNFYGVAYCGAVSWKPEPIALSEYSSAFITQFYGVRDEDFASALTRMARCQG
                     EDLSHTCQARFMFHCDPAEAVWSMAGANEETLLFWNTLGTETRAVHEVLGAVQAAENA
                     DYVRSIDLAARMLNTSAEMALAFHRIGAAMEQAEYDREEAAAALESLARTHGALWDEY
                     EAVGRATNRPINLNHIGRVWSGTTEGLSALAAEVRDGRFPAPVLPGPQVAFNFDGAGQ
                     DAWRDALGTGLELDPAAGDPPVSLESGGPGGSGGYLSLQHGARCEVVDSNRILDFRTA
                     PFVVEAWVRHRGQQEQQYGATIFSFGIGGGWRLGINHLGEVLFTLYGIGERAGTQSIV
                     PADGEWHHVAVNFHHCGQIDYYIDGALTDQLELKGYPHSPGNPLVRVGNEIALVTPFE
                     GDIDRIHIGVGIIGPEDLDSKP"
     gene            complement(6696..6818)
                     /locus_tag="PKW60_02360"
     CDS             complement(6696..6818)
                     /locus_tag="PKW60_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98305.1"
                     /translation="MACVGGAEGSTRLVCRGSREQVEKALAIAESIQGEPAFLE"
     gene            complement(6895..8040)
                     /locus_tag="PKW60_02365"
     CDS             complement(6895..8040)
                     /locus_tag="PKW60_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98306.1"
                     /translation="MKGVLTFLLCGAAALMAWDGAVFAESGGDHVLVGAIRWDAWHGT
                     ESEVGLLVEKTLAPARWHYRLPFFGRVTGKNEVEVRGHTPEIMEQEIAFAQQAGIDYW
                     AFVTYPEDHALSLSLKLYLASEHKHQVNFCVNLQGGWEGGGGLPAWRAKVERYVRYFS
                     EASYQRVLDRRPLVYLYSVDGLVGPGQFADWEEARSAFDELRASAQAACAGNPYIVAQ
                     GWSPETLKEQAAALGLDAIGAYASTEGALAAPYAELAAHTERWWDQFRATGCEVVPLV
                     SAGWDMRPRIETPVPWVKGGDIRKYYDAPSPEELGRHLENAIAWCRQYPEAARAKAVL
                     IYAWNEFDEGGWICPTLTEGTSRLDAIGRVLRDARSDSSEAARETPK"
     gene            complement(8147..9343)
                     /locus_tag="PKW60_02370"
     CDS             complement(8147..9343)
                     /locus_tag="PKW60_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98307.1"
                     /translation="MKPQSRRTFMQTTAAYAGFTGLGRLAAAGEKPIQGFDETAATTQ
                     EGAVWTPVSDRKIRVGIVGYGVCRFGAAFSFQDHPNVEVAAVSDLFPDRCSELAKVCR
                     CEKTYPSLEELIKDDSIEAVFVATDAPSHARHCIAVLEHGKHVASAVPAVFGSLEDAE
                     RLLDAVKKSGLLYMMFETSAFRNDCYAMRQVYNAGGFGQMIYTEGEYFHYSPTPIDSY
                     KDWRLGAPPQWYPTHANAYYTCVTGGTFTHVSCQGMPSTLERFQSGRNAYNNPFGTEV
                     ALYQTSEGGMARMVESRDIPGSHGEVGRVRGQKGSMFDMAYDGLVDLSGVTLAKPPLP
                     PGVEAGGHGGSHGYLCEEFVTAILQERKPLVDIATALNTTVPGIIAHESALKDGERMK
                     VPQYHL"
     gene            complement(9589..10401)
                     /locus_tag="PKW60_02375"
     CDS             complement(9589..10401)
                     /locus_tag="PKW60_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ98308.1"
                     /translation="MPGTLRKAWAFIKRDFQSESSYKLNYFLHVASSLLWIVLFYFLS
                     RIVDSSQASLAKYGGNYFSFVLIGLAFSRYFQVALGVFSGSIQRAQATGCLEAMLSTQ
                     TSPQACVLFSSLYALLESMVQLAIVLIAGVLFFQVDFTQANIPAALTIFVLALLTFVS
                     LGVMSAAGIVVLKKGDPVGWILTSASMILGGAYFPIEVMPGWLQAIAKIIPATYALDA
                     LRLTMLRGHGLGDVSSQVLILGGMVLVLLPVSLRLFDMAVNIAKKDGTLAQY"
     gene            complement(10405..11172)
                     /locus_tag="PKW60_02380"
     CDS             complement(10405..11172)
                     /locus_tag="PKW60_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98309.1"
                     /translation="MISLENVTVRYPIPKRYRELLLHPFRRARRVTALDNVSLTMDSG
                     DRIAFLGPNGAGKTTLLKLVGGLLLPTEGTVRVHGLDTAVDNTAARRSVGFVMNEERS
                     FYWRLTGVQNLEFFGVLDNLSGRDLQQRIDELIELVGLQESAHQNVASYSSGMRQRLA
                     IARGLMTDPDVLILDEPTRTLDPVAAEDLAQLISDRIHRDGKKTLLIATHRLDEVATL
                     CRRMCVIQKGHVVTIDDVAEIQGRDGSLLAYYREQVA"
     gene            complement(11225..>12031)
                     /locus_tag="PKW60_02385"
     CDS             complement(11225..>12031)
                     /locus_tag="PKW60_02385"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPJ98310.1"
                     /translation="EEDVARAKRILLDDGYTQTADLTPEEEQRVRKQNDEEVFHSPRH
                     GLEIELHWRLMPPAVGKASPSEFVWEHARTETRFGMPMLTLPAELHALVIFLHAGVKH
                     RWAQLKFLADAARLMNLPAGLDWGLIWREMNDRRESHVLLVGACLAQTLLGAPVPEIV
                     AAGFQRDARLRASVGLAIGRLFRDGFGLPGLEEWRGYTRALHGLNIDGQPAWTPLGER
                     LRYARSVLTPEYGDKFLLPGLPQGLSFLHYAVRPVRLFSRNGLRLFGRMR"
BASE COUNT         2408 a         3870 c         3475 g         2278 t
ORIGIN      
        1 gcgtttgcgg agcaattgcc cgatcggacc ggtgaactgc gcgaaccctg ccgcaccagt
       61 ggccgcggag gccagtacct catcgtccac accctttcct cgcggtaccc gctcaaaaaa
      121 gcgaacgacg aatgccaggc cctgcttgaa cgctgggccg aaccggccgc gctcatggcc
      181 cggatgaagg gtggagaacc caacctccag tatatcgacc tggcctggga gtacctcatc
      241 aaaaaccatg cgcacgattc catctgcggc tgcagcctcg accaggtaca ccgcgacatg
      301 cggtaccggt tcgatcagtg ccggatgctg gcagacgggc tggtgcgccg catcgtcgcg
      361 gacatcggcg gcgcgagcga ttcccccgaa gccctggcca acaccgtgct ccacaatcca
      421 ctgccgcatg aacgccacgg cgtcttcgaa ctagcactgc cgttccccgc ggactatgcc
      481 ccgaaatatg ttgacggcct cgtcaccggc gagcgggtca accgcttccg gctcctcacg
      541 gcggacggca cgcccatacc gttccagatg gcgcgcatcg agcacggcgt gttgtccaag
      601 cggatccgca ataacgggcg ctacaccaat ttcgagggcg atgtctatca cgtggccgcg
      661 gagctgacgc tgccggcctg cggttatacg ggcatcaaga tcgagcccac cgaagcagcc
      721 accacccgca ataccggcac gctcctgacc ggaatgctca cggcggataa cggacacatt
      781 gagctctcgt tccaatctga cggcaccgca tccttgacgg aaaaggcctc ggggaagacc
      841 tacggcccgc tcttcatgta tgaagatacc ggcgattggg gcgacggctg gacccgcggc
      901 ccgctcgtca aggacatcgt cttcaagacc ccggggaaca acgtgtcggt ggccgtggat
      961 gaagacggac cgctccgcac ggtatttcgt atcgaacgcg agctgcatct gccgcggatg
     1021 gtcgataaag ccacccgctg gcgcgctgac gaccgcatcg ccttgcgcat aaccgaccgg
     1081 gtgtacatcg acaaggacac tccatacgtt cgggtgcgca cgcgcgtcga gaacaccgtc
     1141 gaggaccacc gtttgcgtgt gcttttcccc acccccatcg cgaccgacac gtcatttgcc
     1201 gagaccccgt ttgcgatcgt ggaacgggta atcgatccgc cgccggagac tgcggaatgg
     1261 cacgaacgcg tcaatccgga aacggcgttc acgggccttt tcggcattca ggatgccgaa
     1321 ggcgggctcg ccgtgctgtg cccgcgcggc cttcacgaat acgaggtcac ccggacttcg
     1381 cagcgcgtgc ttgccctgac gctgttccgg tcaacgtacc agaccatcgg cacgcccggc
     1441 gaacccgacg gccagcttct tgaaccgatg gattttgagt atctcctcta tccgtttgct
     1501 gcgccgttca accctgttgc ggccctgcgg accgtgcagg aagcacaaac gcccatccgc
     1561 agccatcagg cggacgctct gcccgggacc ttctccttct gccgagtgga gagcgcgcgc
     1621 gccgtggtca ccgcactgaa acctgccgcg gatcgcaacg gcggcatcgt gcggctctgg
     1681 aacccgggca caacccccgc ggaagccagt gtcgtcttcg cgcagagcct gaagactgcc
     1741 catacctgca acctaaacga agaggcgcag gcggaactgg cgcttggcga tggtggcaag
     1801 caatttgccg tggagatccc cccacatgcc ctgagcaccg tgcgtttcac ctgttaattc
     1861 caggcaccta cgctggacaa cagggacacg atatggacgc tgcttccatc ggaaaatggc
     1921 tcgtgatatc tggcctcggg cttatcgttg ttgggggcgc gatctggctg ctcgcgaaaa
     1981 gcgggctccc cctgggacgc ctgccgggcg acattcacgt cgagggcagg cattcgtcgt
     2041 ttcaccttcc cctggtgacc tgcctcgtca tcagcgctgt gctcacgctg ctcctgaacc
     2101 tcgcgctctg gctgtttcgc aaatagaagc gggcgcggcc ttctttgagc gccgcgcccg
     2161 ccttttttta cccgcgcagt gagggggttc aagcctcctc gacaaccttc catccggccg
     2221 catcggcaag cgcaaatacc ggttccttga gatcgccgta aaaggtgacc cggtgccagc
     2281 cccactgatc ccacatggag aacagttttt cgatatcccc gacaggtttc acgcccagct
     2341 tcgtccggca ggcgcgatcg ttccgatcgt tttcgaccgc tttcccctgg tggaacagga
     2401 cttccttgcg atcggaggag agttcaaggg aggtggtcat gtaacccagc gggaaaatgg
     2461 agcggacgga cgcgccctgg cgatcttcgg aatgggtcag tatctcgaac ggattggccg
     2521 gaccctgcgg gccaaaaggc cggttcgagg cgacgcaatg ggcataaatg atctcgccct
     2581 tcgccgtatc gatcaccggg tcggaaatat agccgggccg cccttgcgtc aaggccgaga
     2641 acgtcaccat cgtcgctgtc gaggggatat cgcactcgca cgcgccgatc aggccttcgt
     2701 tgttcagctc atgaaatccc aggcacggat acgcgtggat gtggccgcca taaaatccgc
     2761 cgaggcagtt gatggtgatc gcattcgcgc cgtatttttt gagcacggcc ttctggccca
     2821 gatacatggc ggccgaagtc tcgagcgtct cccgcgacac gccctcgatt actgcggcgt
     2881 ccttttccca tcgatcggca atggcgcgcg cttcgtccct gtccgcattg gcccaggcct
     2941 cgttgacctc ggcaaaagcc acccgcgtga tcggtatccc catgaactcc ccgccgtctc
     3001 ccgcttcctg atcacgcacg gcgaggatcc gcgacgcctt catgcgggcc agacaatcct
     3061 cgggcgacaa aaagtcaatg ggatcacctt tgcacgtcag ccgacccggc ttgggcgtcg
     3121 acttgatacg cgcccgcgcc gtcgccgcgg cgaaatccgc cggggaaccg cccttccgca
     3181 ccacgtcgaa gcaccggacg gccgcgatct gatctttcat gtccgaggaa gcgacaaacc
     3241 cgacgttggg cgtactgttg cgcagaaaag ccgcggtata gaccaggaat ccgccgctgc
     3301 ccgcatactg gaaatcggcg tacaaaacgg gtttgccgga ggtcgcgatg gtctgcacga
     3361 cctggttcca gcagttcatc tgatagacga tgtagccatc gatcttgccg gaggcgtctt
     3421 cctcgaggat ttgctgcgcc tgttccgggc ccgtggccag ggtgggcaaa aactcatatt
     3481 tgggaaatgc cgcggccagc tcgctgttga tacgctccat ctgcggacga aagtcataac
     3541 cgacgttcgg ccagtcaggt ccgggctgca cctccgcatg aagcgaataa atggtccgaa
     3601 tccgcatctt ttcggcggtt ctggccgctg cagccgcgct tttcgccggc gtacacatcg
     3661 tcgcgcccag acaagccgcg cagcccgatg ccagaaaacc gcggcggctc atgcccagac
     3721 atccggagag tttcggaaac gttttcatcg gaaaattccc ctatccgttg cgcggagaat
     3781 gggcccgcaa ctccaccctg cccagcggcc agtccagtct acatcccgca tgccgcccaa
     3841 ggatcatttt tactctatcc caggcccggg gagaagtcca gatttttcgc tgccgcttca
     3901 ccgacagaaa gtaatccgca ctcgttcccc tggaaatgga caagacatct tcaaaccccg
     3961 gcccaggtgc ttagggtttg gagtccagat cttcggggcc gatgatgccc acgccgatgt
     4021 ggatccggtc gatgtcgcct tcaaaggggg ttaccaaggc gatctcattg cccacacgaa
     4081 ccagcgggtt gcctggcgag tgcggatagc ctttgagttc gagctggtcg gtcaaggccc
     4141 cgtcaatgta gtagtcgatc tggccgcaat ggtggaaatt taccgcgacg tggtgccatt
     4201 cgccatcagc cgggacaatg gattgggttc cggcccgttc gccaatgccg tacaaggtaa
     4261 acaggacttc gcccaggtgg ttgatgccga gacgccaacc cccgccgata ccgaaactga
     4321 agatcgtcgc cccatactgc tgttcctgct ggccgcgatg ccggacccag gcctccacga
     4381 cgaacggcgc cgtgcgaaaa tccaggatgc ggtttgaatc gaccacttcg cagcgggcgc
     4441 cgtgctgcag gctcaggtag cctccgcttc cgcccggtcc gccggattca agcgatacgg
     4501 gcggatcccc ggctgcagga tcaagctcca ggccggtgcc caaagcatcc cgccaggcat
     4561 cctggcccgc tccgtcgaaa ttgaaggcga cctgcggtcc cggaaggacg ggtgcgggaa
     4621 agcggccatc gcggacttct gcggcgagcg cgctgagccc ctcggtcgtt cccgaccaga
     4681 cgcggccgat atggttcagg ttaatcggcc ggttggttgc ccgccctacc gcttcgtatt
     4741 catcccacag ggcgccgtgc gtgcgggcaa gagattccaa cgcggcagca gcttcttccc
     4801 gatcatattc ggcctgttcc atcgcggcgc cgatgcggtg aaaggcaagg gccatctctg
     4861 ccgacgtgtt caacatccgg gcagccaggt ctatcgagcg cacatagtcg gcgttttccg
     4921 ccgcttggac cgccccgagg acttcgtgaa cggcccgcgt ttccgtgccg agtgtattcc
     4981 agaacagcag cgtctcctca ttggcgccgg ccatgctcca aaccgcttca gccgggtcgc
     5041 aatggaacat aaatcgcgcc tggcaggtgt ggctcaggtc ttcaccctgg cagcgggcca
     5101 tccgggtcag cgccgaggcg aagtcctcgt cccggacacc atagaactgg gtgataaacg
     5161 cgctcgaata ctctgacagg gcgatgggtt cgggtttcca tgagacggct ccgcaatacg
     5221 ccacgccata aaaattaagt tcgctcaagt tggtgcccgc cgccccctta tagccatcgc
     5281 cccagctgct cacaaaatgg cccagggcgc cttcgttcgc cgcctgagct gcgagcgttt
     5341 ccatattttg aaacgcgggc gggtagatgg gataaagatt gttccaatcc cacaaacccg
     5401 acagcgccgt gaacgtcatc ccgctttcct tgagggttgt gagccccgga taaacgtcgc
     5461 gcggcgcata gtcccagaac atcggcacga tgtccccggg caagagatcc aggatgccgg
     5521 gatgattcag gatgatgtcc gcgtacatca tcatggtttt gccctgcgat ttaacgagtt
     5581 cgttcatttt tgtgtagtag tcggcgaaaa cgcgttcaag tcctttttcc ctgacggcgt
     5641 cggcggagcg ccccttccct atcgcgtagc tttcatcaag gccgcagtgg aagtattgcc
     5701 cgtcaaacgc gggcgcgatc tccgtgacca tgttgcggac gaggtcgaag gcttcgtctg
     5761 acgttgcgca caagtcgccg cctccttcag gcatttccgc gaggtgccgg tatcgcggct
     5821 cacgaagcgt catctccata tgccccagca gctcgaccgt tgggatcacg tcgatatagt
     5881 attcccgggc gtaggccacc agctcccgga tctcctcgaa gcggaaccgg tcgtattcct
     5941 ggcccagctc ggggtcgaac ccatagacga agtggtcggg ttcgatgaac cacaggtagg
     6001 tgtcgatttt gaaacgcgcc aggagtcgaa tggcgcgttt atgctcgtcc atcgtgggca
     6061 gctgatcgcg cccgtaatcc tcgctgtacc cgcgcatggt cagggcgggc caatcgagga
     6121 tgcgcatgca gggaagggcc gtccgctctc tcagctgtgc actgcgaagc agttgttcca
     6181 gcgtcatcag gccataaaac aacccttttt ccgaggccgc gccgattagc acgctggacg
     6241 gagtgacggt cagcgcatac ccctggtccc gggcggctgc gggcagtacg gtggtcggag
     6301 cgaatggcgc gggcgtccca aaaaacgaga catacagaag atacgtctcg cctgaagccg
     6361 ccgcgggcgt gatgttcaga tccagccgga tcaacgattc gatgaggcgg tctgtcaacc
     6421 gcttgatctg ctcgtcgccg ggagcggtca acatgatgtg catctcgtcg gtgttcagga
     6481 ggcattgacc gggcaggatt tgtaccttct gtggccgggg aagaagatcg ccaaggagcg
     6541 catcatcggc caccgcaccc atcgagacaa acgcgagaat gaatgacgga acaagccagg
     6601 ttttcatacg taacacctct gaagtttcgt gtcaatcact gaaaacgcgt gcgttattca
     6661 ggccgcgggc acatccactc gaactataac caccgctatt cgagaaaggc cggctcgcct
     6721 tgaatggatt cggcaatggc cagcgccttt tccacctgtt cacggctccc ccggcatacc
     6781 agccgcgtcg acccctcggc cccgccaacg caggccatga taccacccgc acaacgagcc
     6841 tcccaagccg ctccgcttgc gcctgcccgt tggcaagcac acggacgtcc gccgtcattt
     6901 cggagtctcg cgagccgcct ctgacgaatc agacctggca tcccgtaaaa cccgtccgat
     6961 tgcatccagc ctcgacgttc cttccgtcaa cgtcgggcag atccacccgc cttcatcgaa
     7021 ttcgttccag gcatagatca ggacggcctt tgctcgcgcg gcttccggat actgtctgca
     7081 ccaggcgatg gcgttttcca ggtgtctccc cagctcttct ggagatggcg cgtcgtagta
     7141 ttttcgaata tcgccgcctt tcacccaggg cacaggagtc tcaatccggg ggcgcatgtc
     7201 ccagccagcg ctgaccagcg gcacaacctc gcaacccgta gccctgaact ggtcccacca
     7261 ccgctcggta tgtgccgcga gttccgcata gggtgcagcc agggcgcctt ccgtggaggc
     7321 atacgcaccg atagcgtcca ggcccagcgc agcagcctgt tctttcagag tctccgggga
     7381 ccagccctgc gccacgatgt agggattacc ggcgcaggcg gcctgagcgc tggcgcggag
     7441 ctcatcgaac gcggaacgcg cctcctccca gtctgcgaac tgcccgggac cgaccagccc
     7501 atccacggaa tacaaataga ccaatgggcg gcgatccagg acgcgttgat acgacgcttc
     7561 gctgaagtag cggacataac gttcgacctt agcgcgccag gccggcaggc cgccgccgcc
     7621 ctcccatcct ccctgcagat tcacgcaaaa attgacctga tgcttgtgct cgcttgcgag
     7681 gtagagcttc aatgagaggc tgagcgcatg gtcttccgga taggtcacaa aggcccagta
     7741 atcgatcccg gcctgctgcg caaacgcgat ctcctgctcc atgatctccg gtgtatgtcc
     7801 gcggacttct acttcatttt tgccggtcac cctgccgaaa aacgggaggc ggtaatgcca
     7861 gcgggccgga gcgagcgtct tttcaacgag aaggcccact tcggattccg tgccgtgcca
     7921 ggcatcccag cgaatcgcac cgaccagcac gtgatccccc ccggactccg cgaaaaccgc
     7981 accatcccac gccatcaggg ccgctgcacc gcacagaaga aacgtcagta cgcccttcat
     8041 gaaagccccc attgattgaa gatcgtcggc gtgggtactc ctaactcgtg ccagcatacc
     8101 cagccgtaga ctcgtggttc gcaaccagac cggccgttcc cggcgtttac agatggtatt
     8161 gcggtacctt catccgttcg ccgtctttca gcgccgattc atgcgcgata atgcccggta
     8221 ccgtggtatt cagcgcggtt gcgatatcca ccagcggctt ccgctcctgg agaatcgcgg
     8281 tgacgaattc ttcgcacagg tagccatgcg aaccgccgtg gccgccggcc tccacaccgg
     8341 gcggcagcgg cggtttggcc aaggtgacgc ccgataggtc cacaaggccg tcataggcca
     8401 tgtcgaacat cgaacctttc tgacctcgca cacgcccgac ctcaccgtgc gaaccgggaa
     8461 tgtcgcggct ttcgaccatg cgcgccatgc cgccttcgct ggtctgatac aaggcaacct
     8521 ccgtgccgaa ggggttgttg taggcattgc gtccggactg gaaccgctcc agggtgctgg
     8581 gcatcccctg gcaggagaca tgcgtaaaag tgcctccggt aacacaggta tagtaggcgt
     8641 tggcgtgcgt gggataccat tgcggcggcg ccccgagtcg ccagtctttg taggaatcga
     8701 tcggcgtggg cgaatagtga aaatactcgc cttccgtgta gatcatctgc ccgaagccgc
     8761 ccgcgttgta gacctgccgc atggcgtagc agtcgttacg gaatgctgat gtctcaaaca
     8821 tcatgtagag caggcctgac ttttttacgg cgtcgaggag ccgctcggcg tcttccagcg
     8881 acccaaacac ggcgggcacg gcggacgcca cgtgttttcc gtgttcgagt accgcgatgc
     8941 aatgccgggc gtggctcggc gcatcggtcg cgacaaaaac ggcttcaatg ctgtcgtctt
     9001 tgatcagttc ctccagcgac gggtaggtct tctcgcagcg gcacactttg gccagctcgc
     9061 tgcaccgatc cgggaacaaa tcgctgacgg ccgcaacttc cacgttggga tgatcctgaa
     9121 agctgaacgc cgccccgaag cggcataccc catagcccac aatgccgacc cggattttgc
     9181 ggtccgaaac aggcgtccac acggcgcctt cctgcgtggt cgcagccgtt tcatcgaatc
     9241 cctggatggg cttctctccg gcagctgcaa gccggcccag tccggtaaaa ccggcatagg
     9301 ccgcggtggt ctgcatgaac gtcctgcgtg actgtggttt catggtttca catccttccg
     9361 taatgctctc tctgcggtat gcgccccgga atcccacgag ttttagatta cacggattat
     9421 aggccagagc ggagcgccat gcacgttgcg taagaatgca gaattttctt cagaggccgc
     9481 cttacacact ttcagcagcg tggggtacgc catttttgcc gcagccaact gcaggtcacg
     9541 gtattacaag gcggatcatg ggaggggaaa agcagagaca gaactgaatt aatactgcgc
     9601 cagagtgccg tctttctttg caatatttac cgccatatcg aacaatctca gactgaccgg
     9661 cagcaacacc aggaccatcc cgccgagaat caggacctgc gaggagacat cacccaggcc
     9721 atgcccgcgc aacatcgtaa ggcgcagggc atccagcgca tacgtcgcgg ggatgatctt
     9781 tgcgatcgcc tgcagccatc ccggcatcac ttcaatcgga aaatacgcgc cgcccaggat
     9841 catgctcgcc gaggtcaaga tccatcccac cggatcgcct ttcttaagca caacgatccc
     9901 ggcggcggac atgacgccca ggctgacaaa ggtaagcagg gccagcacga aaatggtgag
     9961 ggctgcaggg atattcgcct gggtgaaatc cacttgaaag aagagcacgc ccgcaataag
    10021 aacgatcgcc agctgcacca tcgactccag gagggcatag agcgacgaaa agagtacgca
    10081 ggcctggggg gaggtctggg tgctcagcat ggcctccagg catcccgttg cctgtgcacg
    10141 ttgaatcgag ccggaaaaga ccccgagcgc cacttgaaaa tagcgggaaa aggccaggcc
    10201 tatcagcaca aatgaaaagt agttgccccc gtatttcgcc agactggcct gcgaggagtc
    10261 gacgattctg ctcaggaaat aaaacagcac gatccacagg agcgaagaag ccacatgcaa
    10321 aaagtagttg agcttataac tcgactcgct ctgaaagtcg cgtttaataa atgcccatgc
    10381 ttttcggagt gtgcccggca tggcctacgc cacctgctcg cggtaatagg ccaggagcga
    10441 tccatcgcgc ccctggattt ccgccacgtc gtcaatcgtc accacgtgcc ccttctgaat
    10501 gacacacatg cggcggcaga gggtggccac ttcatccagc cggtgcgtgg caatcaacag
    10561 ggtctttttt ccgtcccggt gaatgcggtc ggagatgagc tgcgcgaggt cttcggcggc
    10621 cacggggtcg agcgtgcgcg tgggttcatc aagaatgagc acatccggat cggtcataag
    10681 cccgcgggca atggccagcc gctggcgcat gcccgacgaa taactggcta cgttctgatg
    10741 ggcactctcc tgcaagccca cgagttctat caattcgtca attcgctgct gcaagtccct
    10801 gcccgacagg ttatccagca cgccaaagaa ctccaggttc tgcaccccgg tcaggcgcca
    10861 gtaaaaactc cgctcctcgt tcatgacgaa tcccaccgaa cgccgggcgg ccgtattgtc
    10921 aacggccgta tccagtccgt gtacccggac cgtgccctct gtcggaagga gcaaaccgcc
    10981 gacgagtttg agcagggttg tctttcccgc cccattgggg ccaagaaacg caatgcggtc
    11041 gccgctgtcc atggtaaggc tcacattatc cagcgccgta acccgtctgg cccggcggaa
    11101 cgggtgcagc agcagctccc ggtagcgttt cgggatggga tagcgtaccg tgacattttc
    11161 cagactaatc atggcacatt cgacccgttt cacatccgag cagcatgcgt cgcgataccc
    11221 ccggtcaccg catccggccg aacaggcgca gcccatttcg gctgaaaagc cgcaccggcc
    11281 gtacggcata gtgcagaaat gaaagcccct gaggcaagcc cggcaacaaa aacttgtccc
    11341 catactccgg ggtcaggaca gaccgagcat agcgcaggcg ttcgcccagc ggagtccagg
    11401 cgggctgtcc gtcgatgttg aggccatgga gggctcgcgt ataaccgcgc cattcctcaa
    11461 gccccggcag gccgaatccg tcacggaaga gccgcccaat ggccaggccc acgctcgcgc
    11521 gcaggcgtgc atcacgttgg aacccggcgg ccacgatctc cggcacaggc gcgccgagca
    11581 gcgtctgtgc caggcaggcg cccaccagca ggacatgact ttccctccgg tcgttcattt
    11641 cgcgccagat gagaccccag tcaagtccgg cgggaaggtt catgagccgg gccgcatccg
    11701 ccagaaattt gagctgggcc caccgatgct tcacaccggc atgcagaaag atgaccagtg
    11761 cgtgcagttc tgccggcagg gtcagcatgg gcatcccaaa ccgcgtctcc gtgcgggcat
    11821 gttcccagac aaactccgac ggagaggcct ttcctaccgc cgggggcatc agacgccagt
    11881 gcaattcgat ttcaagcccg tgacgcgggc tgtgaaacac ttcctcgtcg ttctgttttc
    11941 tgacgcgctg ctcttcctcc ggagtcaggt ccgcggtctg agtatagccg tcgtcaagga
    12001 ggatccgctt cgcccgcgca acatcctcct c
//
LOCUS       DAOPBQ010000038        12023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_5256_length_12023_cov_3.779245, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000038 DAOPBQ010000000
VERSION     DAOPBQ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12023
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5256_length_12023_cov_3.779245"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PKW60_02390"
     CDS             <1..1038
                     /locus_tag="PKW60_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPJ98311.1"
                     /translation="APDRIQPVFDWTDWDRAMTRAIDELHFNSFKLPVQGLGGGTFHS
                     RYEPSLLGYAEDTPEYRHIFRTYVQGLQEHLRERGWLDEAYAYWFDEPDPKDYEFVMN
                     GFRKLKENAPDINRMLTEQIEPELIGGPNIWCPLTPAYNFEAAEERRAAGDMFWWYVC
                     TGPKAPYATLFIDHPGTELRVWLWQTWANKVEGILVWQSNFWTSTVAYPDARQNPYED
                     PMGWVTGYSTPAGTRRPWGNGDGRFIYPPEAAADGNSAAPVLEGPVDSIRWEMLRDGV
                     EDYEYFVILERLFESRWEQIPVEERGLYEGLLEVPDSISRNLTEFTRDPAPLETRRDA
                     IARAIEALRRL"
     gene            complement(1131..2210)
                     /locus_tag="PKW60_02395"
     CDS             complement(1131..2210)
                     /locus_tag="PKW60_02395"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPJ98312.1"
                     /translation="MSKYTVALIGGDGTGPEVLAEAMKVVRAAADRCNITLETTDYDF
                     GGNRYLATGEILPDSALDEFRRHDAILLGAIGHPDVKPGILEKGILLRTRFELDQYIN
                     LRPVKLYPNVETPIKNKGPEDIDFVVVRENSAGLYTGFGGVQKKGTPHEVAEQCMVYT
                     RFEVDRCLKFAFDIARKRNKANTLTLCGKTNVLTYVYDLWERAFHEMGAAQYPDIKRE
                     YAHVDAITMWMVKNPEWFDVIVTGNMFGDIITDLGAMVQGGMGIAAGGNINPEGVSMF
                     EPIGGSAPKYTGLNVINPLACIFAGQMMLDYLGEQEAARLVETACVDFLKSGRLTGLS
                     AREIQECGLSTTQIGDNIANRVATL"
     gene            complement(2362..3663)
                     /locus_tag="PKW60_02400"
     CDS             complement(2362..3663)
                     /locus_tag="PKW60_02400"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPJ98313.1"
                     /translation="MRISVIGTGYVGLVAGTCLADTGHRVICVDIDKDRVGRLKRGEM
                     PIYEPGLEELLTRNIEEERLSFTCDLREAVSQSLLVFLCVGTPLLESGEHDLSALIDA
                     AEQVARVMDGYRIIVNKSTGPAGTTARLADRMRELTSHEFDVVANPEFIKEGAAVDDF
                     LRPDRIVVGCKDIRVLEIMRELYAPFLRTGKPFLSMDLASAEMSKLAVNAMLAARISV
                     MNEFAIFCEAAGADVSHVREAVAADGRIGAGYLFPGLGFGGSCLPKDVIASAAWAKSM
                     GLDASMLQAAAAVNNTASRRFIERILSFYGDTVAQKQIAVWGATFKPRTDDLRGAPAL
                     RVIDALLDAGMAIHAYDPVAGKQLAALYGDRIRVAAKNYDALAGADGLVICTEWNEFR
                     RPDYDRMAGLMRERVIFDGRNLYTPATLKQNGFQYFSVGRG"
     gene            complement(3799..4761)
                     /locus_tag="PKW60_02405"
     CDS             complement(3799..4761)
                     /locus_tag="PKW60_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPJ98314.1"
                     /translation="MKLGFLTAMYSNMPLQKVLELTRPLGLEAVELGTGNFPGDPHAP
                     LQELLKSKPKRDELLAMLKGEGMILSALSCHGNCLHPDAKYAQKCQQVQSDTIKLAEM
                     MGVKTVIDFSGCPGSDEGAKRPSWITCPWPPDYLEALEWQWEKKVIPYWTKQAKFAKD
                     HGVQVAFELHPGFVVYNTETMLKLRKACGNNLGANLDPSHLFWQGMDPIDVVRALGKA
                     IFHVHAKDSLVYEQNSRINGVLDTKHYGDEINRSWIFRTCGYGHSIEWWKDFVSTLRM
                     VGYDYVLSIEHEDSLMSNNEGLRKAVECLRQAIISEKVTAITWA"
     gene            complement(5020..6186)
                     /locus_tag="PKW60_02410"
     CDS             complement(5020..6186)
                     /locus_tag="PKW60_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014649513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98315.1"
                     /translation="MAKKKVNVALVGAQFMGKAHSNAWRQVGMFFDAPVEPVMKVLCG
                     KFKEEVAVAEKWGWQETSLDWKEVVNRPDIDIVDICTPNFLHPAIAIEAAKAGKHIVC
                     EKPLANTLKEAQDMLKAAEKAGVKHMCGFSYRFAPAVQTIKNMIVKKELGDIYHFRAA
                     YQQDWIADPEFPMVWRLKKKHTGSGALGDIGAHITDLCHFLVGDVSELAATMETFIKK
                     RVIADSDVGAWGAKGRKKQKKYDTVDVDDAVAYVARIKGANTLATFEATRFAPGRRNY
                     NSIEIYGSKGSVLWNKEDMNVFQYFNCADPATTQGFRKVLATDSGHPYTHAWWPPGHL
                     IGYEHLFVHEIYEFLCGLNRKKNDFPTFADAVKCQKVLDAVEQAAKSKRWVAVK"
     gene            6465..7268
                     /locus_tag="PKW60_02415"
     CDS             6465..7268
                     /locus_tag="PKW60_02415"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPJ98316.1"
                     /translation="MEVVKAVIFAVVEGITEFLPVSSTGHMILLEEFLHFQQSERFPA
                     VFMVVIQLPAILAVVLYFWPTLWPFGKSQEDRDAVFGLWARIAVAVLPAVVLGFLFDD
                     FIEERLFNPLVVACALLAGGVVLLLLERGGRQPRFERAGEAGFGRALAVGFFQCLAMI
                     PGTSRSAATIIGGMLLGASRAAAAEFSFFLAIPTMLGATALKVVKTGAAFTPMEWTLL
                     AIGSAVAFGVAYGVVALFMNYIRRHDFKVFGYYRIALALAVFAYFFFAG"
     gene            7533..8759
                     /locus_tag="PKW60_02420"
     CDS             7533..8759
                     /locus_tag="PKW60_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPJ98317.1"
                     /translation="MRTLLISSFLLALAMSLSLCAAAASLLESEEAAKAETVKLLDAS
                     AGKSRFVSVATVNSDQDLLQDILLVDELGAVWLLLNEGECNFGKPQRIAGPVNSVPGP
                     VSLTLSDVDLDNDEDILIANSLGEIYLVIAEGRGRYRDMVKISQELNAASGPVHVRAS
                     DIDLDNDEDIIVGNSEGVLYKIENVGLGKFGGATMLTRIPGMASGPYDFVLTDLDFDN
                     DEEIILVDSQENVFLLENLRGRFGSPKKIAGPLTKQPGTVQVEVADYNYDGHDDLIIM
                     GGSGAVVLLENKGKGAFSPTPKQLVPPLGPAGGSHIMAMSDMDTTTSEDIVVLNTKGW
                     VFLVENDGQGGARKPQCLTDSLSMAPGAATVTREDLNGDGFEDTLILDGAGNLFLVLG
                     IFDPMEERLRGMEDDL"
     gene            9039..10298
                     /locus_tag="PKW60_02425"
     CDS             9039..10298
                     /locus_tag="PKW60_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPJ98318.1"
                     /translation="MKDRCKALLKEVQHHLREELIPFWMTHGVDEKYGGYLTYFDKDG
                     NPTGETLKTLVCQTRMIFTFSSMFRAGYGGQKALDIARQGVDFLLEHMWDKTHGGWYW
                     TVERDGTPANRSKLGYGHSFGVYALSEYQMATGDPRGLEWAMRNYEVLQTHMADNCYG
                     GYYEFLEEDFQKKRPGKYGGDRKSFDIHMHLMEAFTNLYEASGVHVIHDRTEEMIQLL
                     FARIIDPEFGTGIAQFALDWTPLRAILFADVWGSDRDVDDDAGRPMNNTSFGHNVEFA
                     WLLNHTLNILGKERSGYLEAFRKIYDHCHRYGIDWERGGVYCEGPHDGPAREKNKEFW
                     QQAECLVGMLDAYLLFGDEKYLDAYENVHRFVMDHVINHEVGEWYPLFDENNNRLWDY
                     MGHAWKINYHTVRSMIQCEKRLMKIVG"
     gene            10501..>12023
                     /locus_tag="PKW60_02430"
     CDS             10501..>12023
                     /locus_tag="PKW60_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98319.1"
                     /translation="MPGSRGDISHARPALLCAAFFLFFIQLLTLWIESIYRLSLIKLH
                     PGKELLGLLFVLSPLAALPAGRGAQRRLFPGSLVVMFVARALCPVLGGAALVIAAGAG
                     VGAFLVAVCCLPPERSRVSPESLGLGLTIAVLLSIVLRAWGSSLDISMGRQGDVLAWA
                     LVACAAFLCKGTLSSPETGETASSGTLVDRVAASILLFSNLAVVYLVLSSPAVVTAWS
                     GSSYLIGLLLISFSWGLTLLFCVCGLRSPVPLSRGVLVVWNGAFLAALLSGILLHSVD
                     FPNSPAAPVLIVYPTTWPRHMPFYLMFVLSPVVVFNVMAAFRRAAGSNPRAQVLPVLL
                     GAIFLVVVTLLLIFTNAWGYTGALGHALRNRFYLPFALAGAGMLVPWLLPAWQDAFPL
                     RKASPRCIPLGVFALAIALLAIAGAAYRSASVPHPPAAPNTLTVLTYNMQQGTDVEGH
                     QNLYGQLAFLRAVNADIIGLQESDTARPSQGHTDAPRFFAEGLGYHVFYGPNTISGT"
BASE COUNT         2224 a         3589 c         3600 g         2610 t
ORIGIN      
        1 gcgcccgacc gcatccagcc tgttttcgac tggaccgact gggaccgcgc catgactcgc
       61 gctattgacg agctgcactt caattcattc aagttgccgg ttcaggggct gggtgggggc
      121 acgttccact cccgatacga accgtcgttg ctcgggtatg ctgaagatac gccggagtac
      181 cggcacatct tccggaccta tgttcaggga ctccaggaac atcttcgcga aagaggctgg
      241 ctcgacgaag cctacgccta ctggtttgat gagccggatc ccaaagacta tgaattcgtc
      301 atgaacggat tccgcaagct caaggaaaac gcacccgaca tcaaccgcat gctcaccgaa
      361 cagatcgagc ccgagctgat cggcgggccc aacatctggt gtccgttgac gcccgcgtac
      421 aatttcgagg ccgcggaaga gcgccgcgcc gcgggcgaca tgttctggtg gtatgtctgc
      481 accgggccca aagcccccta tgcgaccctg ttcatagacc atcccggaac cgaactccgg
      541 gtgtggctct ggcaaacctg ggcaaacaag gtcgagggca tcctcgtgtg gcagagcaac
      601 ttctggacga gcaccgtagc ctatccggat gcgcggcaga atccgtatga ggatccgatg
      661 ggctgggtga ccggctacag cactcccgcg ggcacccggc gtccctgggg gaacggggac
      721 ggacggttca tctacccgcc cgaagccgcc gccgatggca actctgcggc gccggtgctg
      781 gaggggcctg tcgacagcat ccgttgggag atgctgcgcg atggcgtcga ggattacgaa
      841 tattttgtca ttctcgagag gctcttcgag tcgcgttggg agcagatccc cgtcgaagaa
      901 cgggggctct atgaaggatt gctggaggtc ccggacagca tttccaggaa cctgacggaa
      961 ttcaccagag atccggcccc actggagacg cgccgcgatg cgatcgctcg ggctatcgag
     1021 gcactgagac gcctctaacg gaggtcatag agaccggcgt agtgccgttt gtggaacagg
     1081 cgctgcaatt tctccgtggc gttcccgggt gaaagggcgc tgctaaaaga tcagagcgtg
     1141 gcaacccggt tggcgatgtt gtcaccgatc tgggtggtcg acagcccgca ttcctggatc
     1201 tctctggccg acaagccggt aagccggccg ctcttgagaa agtcgacgca ggccgtttcc
     1261 accaggcgcg cggcctcctg ctcgcccagg taatcgagca tcatttgccc ggcgaaaatg
     1321 cacgccagcg ggttgatcac atttaagccg gtgtatttcg gcgcgctgcc gccgatcggc
     1381 tcgaacatgg ataccccctc ggggttgatg ttgccgccgg cggcgatgcc catgccgccc
     1441 tgcaccatgg cgcccaagtc cgtgatgatg tctccgaaca tgttgccggt cacgatcacg
     1501 tcgaaccatt cggggttttt caccatccac atggtgatgg cgtcgacgtg ggcgtattcg
     1561 cgcttgatgt caggatactg ggccgcgccc atctcatgga acgcgcgttc ccagaggtcg
     1621 tacacgtagg tcagcacgtt ggtttttccg cacagagtca atgtattcgc cttgttccgc
     1681 ttgcgggcga tgtcgaacgc gaacttcagg cagcggtcca cttcaaagcg ggtgtagacc
     1741 atgcactgct cggccacctc atggggagtg cccttctttt gaaccccgcc aaatcccgtg
     1801 tacaggccgg cggaattttc gcggaccact acgaaatcga tgtcttccgg gcctttattc
     1861 ttgatcgggg tctcgacatt cggatacagc ttgaccggcc gcagattgat gtattgatcc
     1921 agttcaaagc gggtccgcag gaggatgcct ttttccagaa tgccaggttt gacatccgga
     1981 tgcccgatcg cgcccagaag gatcgcgtcg tgccggcgga attcgtctaa cgcggaatcg
     2041 gggaggatct cgccggtcgc caggtagcgg ttgccgccga aatcgtaatc cgtcgtttcc
     2101 agggtgatgt tgcagcggtc agcggccgca cggacaactt tcatcgcttc cgccagcact
     2161 tccgggcccg tcccgtcccc gcctatcaaa gccaccgtgt attttgacat gctcggctcc
     2221 ccttcacgtt gaagacgaat gactctcggg cgtgcaccgc cgccaggaag gcgaaatggt
     2281 agcacgcacc gggggaggat gcaaaaggag gcggggcggc taaaatccaa acgcgccgca
     2341 cttgagtcat cggctgggcc gttatcctct tcctacgctg aaatactgaa atccgttctg
     2401 cttcagggtg gcgggagtat ataagttgcg gccatcgaaa atgacgcgct cgcgcatgag
     2461 cccggccatc cggtcgtagt ccgggcggcg gaattcgttc cattccgtgc agatcacgag
     2521 cccgtccgcg ccggccagcg cgtcgtaatt cttggcggcc acccggatgc gatcgccgta
     2581 cagcgccgca agttgttttc ccgcgacggg gtcgtaggcg tggatggcca taccggcatc
     2641 cagcagggcg tcaatcacgc gcaatgccgg cgcgccgcga aggtcgtcgg tgcggggttt
     2701 gaacgtcgcc ccccagacgg cgatctgctt ctgcgcgacc gtgtccccat agaatgacag
     2761 aatccgctcg atgaagcggc gtgatgccgt gttgtttacg gcagccgcgg cttgcagcat
     2821 ggaggcgtcg aggcccatcg atttggccca ggccgcgctg gcgatcacgt ctttgggcag
     2881 acaggagccc ccgaaaccca ggccgggaaa caggtagccg gcgccgatgc gtccatccgc
     2941 ggcgaccgct tccctcacat gcgagacgtc ggcgccggca gcctcgcaga agatggcgaa
     3001 ctcgttcata acggagatgc gggcggcaag catggcattt acggcaagtt tgctcatttc
     3061 agcgctggcc aggtccatcg acaggaaggg tttgccggtg cgcaggaacg gggcgtacag
     3121 ctcccgcatg atctccagca cacggatgtc tttgcacccc actacgatgc ggtcgggccg
     3181 cagaaaatca tcgaccgcgg ccccttcctt gataaactcc ggattggcga ccacgtcgaa
     3241 ttcgtgggaa gtgagctctc gcatgcgatc tgcgaggcgc gctgtggtac cagcaggccc
     3301 cgtgcttttg ttaacgatga tgcggtagcc atccatgacc cgggccacct gttcggcggc
     3361 gtcgatcaaa gcggagaggt cgtgctcgcc agattccagc aacggcgtgc caacacagag
     3421 aaatacgagg agagactgag acacggcttc cctgaggtcg caggtgaaac tgaggcgttc
     3481 ttcctcgatg ttccgggtga ggagctcctc caatcccggc tcgtaaatgg gcatttcgcc
     3541 tcgtttgagc cgcccaaccc ggtctttgtc aatatcgacg cagataacgc ggtggcccgt
     3601 atcggcaagg cacgtgccgg ccacaagacc cacgtagccc gttcctatta ccgaaatccg
     3661 catggactgg tttcctccgg ggagcgcaga ctgttccggg ggcctcagct gccgcgcgcc
     3721 gcggccccgg atactgtgaa aagggctgcg ggagcccgcg cgatggggct cccgcagcaa
     3781 cgacgcctga tggcggcatc aagcccacgt tatcgcggtg actttttccg aaatgatggc
     3841 ctgcctgagg cattccacgg cctttcgcag cccttcattg ttggacatga gcgagtcttc
     3901 gtgctcaatg ctcaacacgt agtcgtaccc gaccatgcgc agggtcgaca cgaaatcctt
     3961 ccaccattcg atgctgtggc cgtagccgca ggtgcggaag atccacgagc ggttgatctc
     4021 gtcgccgtag tgtttggtgt cgagcacgcc attgattcgg gaattctgct cgtataccaa
     4081 cgaatctttg gcatgcacgt ggaagatcgc cttgcccagc gcgcgcacga catcgatggg
     4141 atccatgccc tgccagaaca ggtggctcgg atcgaggttg gcgcccagat tgttgccgca
     4201 ggctttgcga agtttcagca tggtctcggt gttgtacacc acgaagcccg ggtgcagctc
     4261 aaacgccacc tgaacgccgt gatctttcgc gaattttgcc tgcttggtcc agtatggaat
     4321 gactttcttc tcccactgcc attccagggc ctccaggtaa tccggcggcc agggacaggt
     4381 gatccacgag gggcgcttgg cgccttcgtc ggagcccggg cagcccgaaa agtcgatgac
     4441 ggtcttcacg cccatcatct ccgccagttt gatggtgtcg gactgcacct gctgacattt
     4501 ctgggcgtat tttgcgtcgg gatggaggca gttgccgtgg cagctcagcg cgctgaggat
     4561 catgccctcg cctttgagca tggccagcag ctcgtcgcgc ttgggtttgg atttcagcag
     4621 ttcctgcagc ggggcgtgcg ggtcgcccgg gaaattcccc gtgcccagct cgactgcctc
     4681 aagacccagc ggccgcgtca gttcgaggac tttctggagc ggcatgttgc tatacatcgc
     4741 tgtgagaaaa cccagtttca tagtcgttct ccctcctctg gtttatactt tcggggacat
     4801 gacaacgcgc gccgcgcatc cggccgggcg ttttttacgt tcatgccctc agcctgtgca
     4861 gttcatccag tgtaatcgga cttcccccaa atttcaccca caacaccccg ggagccgctg
     4921 atgcacccaa ccggtgtatg cgccgcgccg ggatggacac gcaatcccgc catcccggtg
     4981 cggccgtttg ctgttttgag gcccggcccc gggctcgagt cacttcaccg cgacccagcg
     5041 cttgctcttc gcggcctgtt cgaccgcgtc aagcaccttc tgacatttca cggcgtccgc
     5101 gaacgtcggg aaatcgttct tcttgcggtt gaggccgcag aggaattcgt aaatctcatg
     5161 cacaaagagg tgttcgtagc caatcaagtg tcccggcggc caccacgcat gcgtgtaggg
     5221 atgccccgaa tccgttgcca gcactttgcg gaagccctgc gtggtagcgg ggtcggcgca
     5281 gttgaaatac tggaacacgt tcatgtcttc cttgttccac agaaccgaac ccttgctgcc
     5341 gtaaatctcg atcgaattgt aattgcgccg gcccggagcg aaacgcgtcg cctcgaacgt
     5401 cgccagggtg ttcgctccct tgatgcgggc cacataggcc accgcgtcgt ccacatcgac
     5461 cgtgtcgtat tttttctgtt tcttccggcc tttcgcgccc cacgcgccca cgtcgctgtc
     5521 cgcaataacg cgcttcttga tgaaggtttc catcgtggcc gcgagctccg acacgtcgcc
     5581 caccagaaaa tggcacaggt cggtgatgtg ggctccgata tcgcccagcg ccccgctgcc
     5641 cgtgtgtttc ttcttcaggc gccagaccat cgggaattcc ggatcggcga tccagtcctg
     5701 ctggtacgcg gcacggaaat gatagatgtc gcccagctcc ttcttcacga tcatgttctt
     5761 gatcgtctgt accgccggcg cgaaccggta cgaaaatccg cacatgtgct tcacgcccgc
     5821 cttctcggcg gctttcagca tgtcctgtgc ttctttcaac gtgtttgcca gcggcttctc
     5881 gcacacgata tgtttccccg ccttggccgc ttcgatggca atggccgggt gcaggaagtt
     5941 cggcgtgcag atatccacga tgtcgatatc cgggcggttg accacctctt tccagtccag
     6001 cgaggtctcc tgccagcccc acttctcggc taccgccacc tcttccttga attttccgca
     6061 caagaccttc attaccggct caaccggggc gtcgaagaac atcccgacct gccgccatgc
     6121 gttgctgtga gccttcccca tgaactgggc gcccacgagg gccacattca ctttcttctt
     6181 ggccatactt ctccctcctc cacggttatt cggttgctgc atttgcagga cttcctatta
     6241 taggaagggt tgcctcgaat gccaagtgcc atctgattcg aaaatccctc aacgtgacgt
     6301 ctgagcaatc ccgcggcatt ttctgactcc cctgctgcgg gtgcgttgtg tgccgggggc
     6361 ccagcggtta tactccgccg tagcacccgg ggcaactctc cggcagtctc ttctgccgga
     6421 tgcggggtga ccaggttctg aggaacagga tgggcgatta gcacatggaa gtcgtaaaag
     6481 cggtcatttt cgcggtggtc gaggggataa cggagttcct gcccgtcagc agcaccgggc
     6541 acatgattct gctcgaagaa ttccttcatt ttcagcagag cgaacgcttc ccggctgtgt
     6601 tcatggtcgt tatccagctg ccagccatac tggcggtggt gctctatttt tggccgaccc
     6661 tgtggccgtt cgggaagtcg caagaagacc gcgacgccgt cttcgggctc tgggcccgga
     6721 tcgccgtggc cgtgcttcct gcagtggtgc tggggtttct gttcgacgat ttcatcgagg
     6781 aaagactctt caacccgctg gtagtggcct gtgctttgct cgcgggcggc gtggtgctgc
     6841 tcctgctcga acgcggcgga cgccagcccc gttttgaacg cgcaggcgag gcgggattcg
     6901 gtcgcgccct ggccgtcgga ttctttcaat gcctggccat gattcccggc acgtcccgct
     6961 ctgctgcgac catcattggc ggcatgctgc tgggagcaag ccgcgcggca gccgcggagt
     7021 tttccttctt ccttgctatt cccaccatgc tgggggccac ggcgttgaaa gtcgtcaaaa
     7081 ccggcgccgc cttcactccc atggaatgga cattgctggc catcggttcc gcggtcgcgt
     7141 tcggggtggc ttatggggtt gtggctcttt tcatgaacta catccgccgc cacgatttca
     7201 aggtgtttgg ctactaccgg attgccctgg ctctggccgt gtttgcatac ttcttctttg
     7261 cagggtaata cgcacgggcg agcgtgccgc tggaattctt cgggcccttt catgaatacc
     7321 ggcaccgcga aatacgtcct tactgtgaag gtgcgcgctc gaattggtat catcgcaaag
     7381 cccggctgcc atttttctgg ctgggcggca agacgcaagc cctgaaccaa aggcgcaacg
     7441 gcatggttgg ccatgattgt ataaggggtt cccccaaagg cgccgcggcc gcgcggatgt
     7501 ggttttgaaa gactcgttcg gagacttttg cgatgcgaac acttctaatc tcgtcattcc
     7561 tgctcgcctt ggccatgtct ttgtccctct gtgcggcagc cgcctcgctg ctggagagtg
     7621 aagaagccgc caaggcagag accgtcaagc ttcttgatgc gtccgccggg aaatcgcgtt
     7681 ttgtctctgt agccacggtc aactccgatc aggatttgct ccaggacatc ctgctcgtag
     7741 atgagctggg cgcggtctgg ctcctgttga atgagggcga atgcaatttt ggtaagccgc
     7801 agcggattgc tggtccggtg aattctgtac cgggccccgt ctcgctgacg ctgtccgatg
     7861 tggatctcga taacgacgaa gatattctga ttgcgaacag cctgggcgag atctatctgg
     7921 tcattgccga agggcggggc cgctaccgcg acatggtaaa gatttcgcag gaactcaatg
     7981 ccgcgagcgg gccagtgcat gttcgcgcat cggacatcga cctcgataac gatgaggaca
     8041 tcatcgttgg caacagcgag ggcgtgttgt acaaaatcga aaacgtcggc ctgggaaaat
     8101 tcggaggcgc cacgatgctc acccggatcc ccggcatggc ctccggcccg tacgatttcg
     8161 tgctcaccga cctggatttc gataacgacg aggagattat cctggtcgat tcccaggaga
     8221 acgtgtttct gctggaaaat cttcgcggcc gcttcggttc tcccaaaaag attgccggac
     8281 cactgaccaa acagccgggg accgtgcagg tggaagtggc tgactacaat tatgatggtc
     8341 acgacgattt gatcatcatg ggcggctcag gggcggtggt gcttctcgaa aacaaaggaa
     8401 agggggcgtt ttcaccgact ccgaagcagc tcgtgccccc gctcggccca gcaggcggca
     8461 gccatatcat ggcgatgtca gacatggata ccaccaccag cgaagacatc gtcgtattga
     8521 acaccaaagg ctgggtattt ctcgtcgaaa acgatggcca gggaggggct cgtaaaccac
     8581 agtgcctgac cgattcactc tcgatggccc caggcgctgc taccgttaca cgggaagacc
     8641 tgaatgggga tggtttcgaa gacaccctaa ttcttgacgg agcgggaaac ctgtttcttg
     8701 tgctgggcat cttcgatcct atggaagaac ggctgcgcgg tatggaagac gacctgtgat
     8761 tccacccagg cgctcctgga gacgttgcgt gcatcgcggc gccgattcac aatctcgaaa
     8821 ttgacctgcc ggaattcttt tctgccgtat ttccggttgt cccagaggca ttttgaaggc
     8881 ttgctgcgtc ttcgatgtac tgtcctggcc cgtgtggcgg cttatgtgct ggcgtggcag
     8941 ccggagtcac ggaagcgttg tcataacttg agaaacgggc aattgcttcc gtagactgac
     9001 gccgggccgc aatcgcaaca ttcaggggat gacacaccat gaaagaccgc tgtaaggcac
     9061 tgctcaaaga agtgcagcac cacctccgcg aagagttgat tccgttctgg atgacccacg
     9121 gggtcgacga gaaatatggg ggatacctga cctattttga caaagacggc aaccccacgg
     9181 gcgaaaccct caaaaccctc gtgtgccaga ctcgaatgat tttcacgttt tcgtccatgt
     9241 tccgtgcggg atacggcggg caaaaggctc tcgatattgc ccggcaggga gtcgatttcc
     9301 ttctggaaca catgtgggac aagacgcatg gcggctggta ctggaccgtc gaacgcgatg
     9361 gcacccctgc caaccggagc aaattggggt atgggcactc gtttggcgtc tacgcgctga
     9421 gtgaatatca gatggctacg ggcgacccgc gcgggctcga atgggccatg cgcaactacg
     9481 aggtccttca gacccacatg gcagacaatt gctacggcgg ctattacgag tttctcgagg
     9541 aagattttca aaagaaacgg cccggcaaat acggaggtga ccgaaagtcg ttcgatattc
     9601 acatgcatct gatggaggcg ttcaccaacc tctatgaggc ctcaggcgtg cacgtgattc
     9661 acgaccgcac cgaagagatg atccagctcc tgttcgcccg catcattgac cccgagttcg
     9721 gcaccggtat cgcccagttt gccctggatt ggacccccct gagggccatc ctcttcgcgg
     9781 acgtctgggg ctcggatcgt gacgtcgatg acgatgcggg gcggcccatg aacaacacca
     9841 gtttcgggca caacgtcgag tttgcatggc tcttgaacca caccctgaac atcctgggca
     9901 aagagcgcag cggctacctc gaggctttcc ggaaaatcta tgaccattgc caccgctatg
     9961 gcatcgactg ggagcgcggc ggcgtctact gtgaaggtcc ccacgacggg ccggctcgcg
    10021 agaaaaacaa ggaattctgg cagcaggccg aatgcctggt gggcatgctc gacgcctacc
    10081 ttctctttgg cgacgaaaag tatctggacg cctacgagaa tgtccaccgc ttcgtgatgg
    10141 atcacgtcat caaccacgag gtcggcgagt ggtacccgct cttcgacgaa aacaacaacc
    10201 gcttgtggga ttacatggga cacgcgtgga agatcaacta tcacaccgtg cgctcgatga
    10261 ttcagtgcga gaaacgtctc atgaagatcg tggggtaggg agcgcgtcac gtcccgcact
    10321 ctgtgttttg tgcgatctct gtggccaagg catccgggtg tccgcctcgg agaacacaga
    10381 gagcgcagag atgtaagagg ggagacctac cgtcgccgga gcgacatggt ctggagacca
    10441 tgccacatca agtacggcgt ggcacggctt tgaagcgatt gcaggagagg gggagtcaga
    10501 atgcccggta gccgcgggga catcagccat gcacgcccgg cccttttgtg tgcggccttc
    10561 tttctctttt tcattcaact gctgacgctg tggatcgaga gcatctaccg gctgagtctg
    10621 attaagctgc atcctggaaa agaactgttg gggctgcttt ttgtgctgtc tcccctggcg
    10681 gcgctgcccg ccgggcgagg cgcgcagcgg aggttgtttc cgggcagtct cgtcgtgatg
    10741 tttgtggcgc gagcgctctg tcccgtcctc ggtggggcgg ctctggtgat tgccgcgggt
    10801 gcgggtgtgg gcgcttttct cgttgcagtc tgctgtctgc cgcccgagag gtcacgagtc
    10861 tcgccggaga gccttggact cggcctgacg attgccgtgc tcctgtctat cgtgttgcgg
    10921 gcgtggggat cctcgctcga catctcgatg ggcagacagg gggatgtgct ggcgtgggcc
    10981 cttgtggcgt gcgcggcatt tctgtgcaag ggcacgcttt ccagccccga aaccggcgaa
    11041 acggcgtcat ccggcacact ggtcgaccgc gtggccgcat ccattctcct cttctcaaac
    11101 ctcgcggtcg tctatctcgt cctgtcgagc cccgccgtgg tgacggcctg gtcgggaagc
    11161 agttatctca taggcctgct cctcatctca ttctcctggg ggttgaccct gcttttttgt
    11221 gtctgcggac tgcggagccc ggtcccgttg tcgcgcggtg tgctggtcgt ttggaatggg
    11281 gcgttcctgg cagcccttct gagcggcata ctgctgcact cagtggattt tccgaattct
    11341 ccagcggccc cggtgctgat agtgtacccc acgacgtggc cccgtcacat gcccttttat
    11401 ctcatgttcg tgttgtctcc ggtcgtcgta tttaacgtca tggccgcgtt caggcgggcg
    11461 gcaggttcga acccgcgcgc acaggtgctg cccgtcctgc tgggcgcgat ctttctggtt
    11521 gtggtcacgc tgctgttgat cttcaccaat gcgtggggct acacgggcgc cctggggcac
    11581 gcgctccgga accggtttta cctgccgttt gccctggcgg gcgcggggat gctcgtcccc
    11641 tggctgcttc ctgcgtggca ggacgcgttt ccgcttcgca aagccagccc tcgatgtatt
    11701 cctctcgggg ttttcgcgct ggccattgcc ctgttggcca tcgcaggtgc ggcgtatcgg
    11761 agcgcaagtg taccccatcc tccggccgcc cccaatacat tgaccgtcct cacatacaat
    11821 atgcagcagg gaacggatgt ggaaggccat caaaacctgt atggccagct cgcatttctc
    11881 cgtgctgtaa atgcggacat catcggcctg caggaaagcg acacggcgcg cccctcgcaa
    11941 ggccacacgg atgcaccgag atttttcgcc gagggattgg ggtaccatgt gttttatggt
    12001 cccaacacca tctcggggac att
//
LOCUS       DAOPBQ010000039        11722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_5400_length_11722_cov_4.527128, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000039 DAOPBQ010000000
VERSION     DAOPBQ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11722
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5400_length_11722_cov_4.527128"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="PKW60_02435"
     CDS             <1..1482
                     /locus_tag="PKW60_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98320.1"
                     /translation="DTRFAGNWLATFSDDRTTASAPYGQIQYAILLNIRQRGTGISGN
                     GTLFRVFREGPTASNRIAVQMSGTTSGDDATFTLRSGAGGLDREQTWYVRLGMDRMAG
                     MYLTADNAGTLSRSGHALWHKATSNARTDVPWAAAYTDEFGIAGFPQRSRTVSVTPIV
                     EQTTLRGSGTMYEHVSLAPELNFDLVRGGVSGSEIGFTFGGLDLIENEVDWFGFFGGN
                     IMVTAYGQFDASDALIRSGHTVWVPAPERELTAITGAWAGSFRDRTVEKSNVPGDFTA
                     VFHLTANDGGAVTGYALMRNEADDSPDFQRYNIDNGSIIGTQVQLSMSRLAGSFFWDL
                     RLCQDRLVGSYQRFAGSQNRLVGTGHAEFRTMTSAGVLRGTWASAYVDTYGAAHPEQS
                     QLAVVTVANQASAGASFSGYGALRFAGESRRRLFNVAGEAASGDIVWEWRGADLFGRT
                     VWHLRRCNNLLYGTYTNYTSGGAIESHGSGVWIKSTFSSSFEQ"
     gene            1600..2061
                     /locus_tag="PKW60_02440"
     CDS             1600..2061
                     /locus_tag="PKW60_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPJ98321.1"
                     /translation="MREQKLKELLDREGIRYVTISHSTAYTAQEVAASAHVSGREMAK
                     TVMVRLDDEMAMVVLPASRKLDFARLKESLGAHNVELAAEDEFRGLFPGCDAGAMPPF
                     GNLYGVKVYIDENLSAEDRIAFNAGSHTEVIRMPYRDFARLVNPRVLSIAR"
     gene            complement(2107..3087)
                     /locus_tag="PKW60_02445"
     CDS             complement(2107..3087)
                     /locus_tag="PKW60_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HPJ98322.1"
                     /translation="MPPERVNLKPAEPVKLIFDTDIGNDVDDALALGVIHALQSRGEC
                     ELLAVTLTKDHERCAPLVDAINTFYGRGGIPIGVTHSGITTQDSKFLSLTRMEDNGVL
                     RYPHDLQSGNDAPDAVTVLRQVLAAQPDHSVAIVQVGFSTNLAALLDTPPDEISPLNG
                     LDLAAAKVRVLSIMAGAFELIHGEEHPEYNVVMDIPAAQKLVDNWPTPIVCSGYEIGL
                     AITYPAESIEQDFSYAAHHPLAEAYQLYNPTPHERPTWDLTSVLWAVRPERGYFTLSN
                     PGRIRYDDKGLSTFEESPDGNHRYLIADAIQATRVREALTLLASQPPDKR"
     gene            complement(3235..5421)
                     /locus_tag="PKW60_02450"
     CDS             complement(3235..5421)
                     /locus_tag="PKW60_02450"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPJ98323.1"
                     /translation="MPGWQFHLFALPLLATSGIAAVFAAVLWRQRSQYGAITLMILMI
                     AACVWTVGEGAELGCQTIAGRLLWSRIRHVGIAVIPLAWLVFAFQFSQGRNRRLPWYF
                     KAFIVFPIVNVALVWTNERHHLIWSSLRLETRAPVMTLAMERGPLYWLLLAYAYTFIL
                     AGTIILVRAAVRSHRIYRRQTLFLLLGTLTPLAANIVFQIGFSPIPHMDLTPLSFGFA
                     ALVIGWNFLRYGLLLPPVPVARNQVIESMKDGVIVFDLGHQILDLNEAARAMLGKSES
                     AILGRPVTDFLTFSQALFDASMHPGKDVETQVQMNDGATRYLEAALSPIRSRWGTPVG
                     SVMILRDVTDRRLAELGQRRLFTAIEHAAEDIVIADSQGIIVYVNPAFERITGYSRNE
                     AVGQNPRILKSGAHSQAYYETMWAALLEGKVWQGTLTNRRKDGTLIQEDATISPIFDY
                     RGALMGYVSVKRDVTQQLALEAQVRQAQKLESLVTVAGGIAHDFNNILAIIAGNAEMA
                     ISGLSQAAGARPYLKEIAGAARKAIELSRQMLAYSGRGKAALELIELNTFIMEMAQLI
                     QTSIADTCRLRFNLARRVPVFEGDPSQISQLIMNLAINASEAIDQTGGLITIATGAVF
                     CTHADLAATWLHENQPEGHYVYLDVTDTGAGMSESAVSRIFDPFYTTKFTGRGLGLAA
                     VLGIVRGHYGAIKVDSTPGSGTTFRVFFPVKEPVVEAPAPVSQGEP"
     gene            complement(5523..8855)
                     /locus_tag="PKW60_02455"
     CDS             complement(5523..8855)
                     /locus_tag="PKW60_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPJ98324.1"
                     /translation="MKGGVNFGSMFGSKRLEFMRLMRILRNALLPAALGGTAVLCALL
                     ASASAVSGAAVAVNEPTVPPLLDTLTPEERAWLDEHPVLVFSIDNDHQPKSYRNEHGE
                     LAGINVDYVRLLEARLGIEIRLEGAPWPEALGKALLHKVDGVVNADLLEERRPYLNFT
                     DVYAVYPQALLTRRDEPAISSLDEFAGRAVAAVRNSSQLAALREGFPDVLIHETDDTD
                     SGIDLLIEGKVGGAFGDLAVLYDSISARFLTNLKLALVYSAPPVGYARIGIRNDDAVM
                     LSVLNKAVDSITDSDRRAIELRWLGIELPRFSPGDSVFQLALTEEERAWLQENPVIHV
                     ASDSQWAPIEFLDDTGQFQGIAIDYLHEIAEMLGLEVVFLRNHSWQDLEKLGRAGQVD
                     LFTAIARTEKRTRHFEFTEPYLSFPVMLFARDELAYVGSLRELYGQRIVVVRSHAIEA
                     LIQQEHPQITIVHADSVVEALDMLQRKDVSVFAAPIVTASHYLSRQGFSNIKVVGETP
                     YVYEQRIAIRASAPLLVSSVRKAFAAIPPPRRHAIYQKWVSLRYDPGFPFSLVWKLAL
                     GVLALLLLVLFWNYRLSRAVSQRTAALSTANEAVRTSEERAQGIISSITDPLLMMNRE
                     RIVTWANGHAKAAFGEALVGKECREVFDCTQLPCEHCPVFRALSTGQVHDSETQILDI
                     HGKLRHFWCTAGVAAHDTEGRPSAVIEILRDITERKQNEMARRILEDQLRQAQKMEAV
                     GQLAGGIAHDFNNLLQVIRGNIELIVNEVPQGSPEQSGLEEVDRAAERATTLVRQLLT
                     FARSEKLEQTPLDLNEVVSGLIKMIRRLIGEHIEMVFEPGPGDVTIFADQGQVEQVIL
                     NLCVNARDAMPQGGRLELEVERREIDEGFTSRFMETQPGRYVVLSVADSGSGVHPDIV
                     EHLFEPFFTTKGVGEGTGLGLATVYAIVTRHLGFLDLESRPGQGAVFRVYFPVYEPGR
                     EALELRHGTSEEAQRGNNETILVAEDDEQVRNLAERVLKRAGYRVLLARDGDEALAAI
                     IEHARDIDLVILDVVMPRRSGGDVYTEIQARFSNTRVLLMSGYSFDVLDKGHLPAGQP
                     DLLRKPFRSETLLRRVHDALAR"
     gene            9021..10097
                     /locus_tag="PKW60_02460"
     CDS             9021..10097
                     /locus_tag="PKW60_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPJ98325.1"
                     /translation="MLSLHFLAGVLAALAAVVAADEPSSIELNGAVYGATADERGPIG
                     GGPGYAAAVTSGDLTADTLETLIEALANAQAGQTVFVPGSAEIDFTTFIYIDKFVLEI
                     PEGVTLASDRGVAGSPGALLFSDTLETCPMIRVNGPGVRVSGLRIRGPNTKRYLDHHD
                     RAFGKGGGGHSYYYKFPVQDGIATVYDGLEVDNCEISGFGHSGVYLTKGQNHHIHHNY
                     IHHCQYNGLGYGISHNESSSLIERNLFDWNRHSIAGSGRPGSGYTACHNVELGTSLSH
                     CFDMHGGRDRKDGTDIAGTRLDIHNNTFRAPKPPIVIRGIPEESCEVYRNWFPLHAPG
                     QAVNAGERTTVTDNAYGADPTQGE"
     gene            complement(10124..10618)
                     /locus_tag="PKW60_02465"
     CDS             complement(10124..10618)
                     /locus_tag="PKW60_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPJ98326.1"
                     /translation="MLAPTIIMGVLAAVLLLAGYMRGEGQHLEGLRSALIMTITVLPL
                     LLFAFVVAGMVQALVPTEIIARWVGAESGLRGIAIGTVAGGLAPGGPYVSLPIAAALV
                     RSGASTGTMVAFLTAWSLWAVARLPMEVGILGWQLMLARIASTFFFPPIAGLIAQFFF
                     DHSK"
     gene            complement(10683..>11722)
                     /locus_tag="PKW60_02470"
     CDS             complement(10683..>11722)
                     /locus_tag="PKW60_02470"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98327.1"
                     /translation="VTVAAYRDLRELLAADDIDAVFVAAPNHWHVLAALWGCQAGKDV
                     YVEKPMAHSIWEGRQLIAAAEQTGRVVQVGTQRRSEPSTQRLVERLQAGVIGDLYAAR
                     VIHYSTRPPVPFHPDEAPPETLDWPLWQGPAPDKPFSKKYVHYDWHWFWHFGNGELGN
                     NGVHYADIVNWVLGKGLPVHIASQGGRYGYEDSAETPNVQTTTATFADGTLFTCEIRG
                     RYSNDENELKSGNLFYGSNGYAVGNTCYDTEGKEIPDENPPRNGDAVLLHLANFHDAI
                     RSRNRDAVAAKPADGHVSAAFCHLGNIAFRLGRDVTFDPQTETFGNDAEANALLVRPG
                     YRSGFEVPQLA"
BASE COUNT         2247 a         3652 c         3633 g         2190 t
ORIGIN      
        1 gacacgcggt ttgccgggaa ctggctcgcc acgttcagcg acgaccgcac caccgccagc
       61 gcgccatacg gccaaatcca atacgccatt cttctcaata tccgccagcg cggcacgggg
      121 atttccggaa acggcacgtt gtttcgtgtg tttcgcgagg gaccaacagc ttcgaaccgc
      181 atcgcggtgc agatgtccgg cacgacgtcc ggcgacgacg ccacgtttac cctgcgttcc
      241 ggcgccggcg gcctggatcg cgagcagacg tggtatgtgc gccttggtat ggatcgcatg
      301 gcgggcatgt atctgaccgc cgacaatgca ggaaccctgt cgcgttcagg acatgccctg
      361 tggcacaagg caacctcgaa cgcccgcacg gacgtcccct gggcggcggc ttataccgac
      421 gaattcggca tagcggggtt cccgcaacgg tcgcgcacgg tttccgtaac ccccattgtt
      481 gagcagacca cgctccgggg cagcggaacc atgtatgaac acgtgagtct ggcgcccgaa
      541 ctcaatttcg acctggtgcg cggaggggta tcgggctcgg agatcggatt tacgtttggc
      601 gggctggatc tcatcgagaa cgaggtggat tggttcgggt ttttcggtgg gaacatcatg
      661 gtcaccgcct acggccagtt tgatgcgtcc gatgcattga tccgatccgg ccatacggtc
      721 tgggtgcccg ctcccgaacg cgaattgacg gccatcacgg gggcgtgggc gggctcgttc
      781 cgcgatcgca cggtggagaa atccaatgtg cccggcgatt tcacggccgt gttccacctc
      841 accgcaaatg acggcggcgc ggtgaccggc tatgcgctca tgcggaatga agccgacgac
      901 agtcccgact tccagcgcta caacatcgac aacggcagca tcatcggcac gcaggttcag
      961 ctttccatga gccgcctggc gggctcattt ttctgggacc tgcgcctctg ccaggaccgg
     1021 ctggtggggt cgtaccagcg ttttgccggc agccagaacc ggctcgtggg cacgggacac
     1081 gccgaattcc gcaccatgac cagtgcggga gtgcttcggg gcacgtgggc atcagcctac
     1141 gtcgacacat acggggcggc gcatcccgaa cagtctcagc tggccgttgt caccgtggcc
     1201 aaccaggcgt ctgcgggcgc gtcgttcagc gggtatggcg ccctgcgctt cgcgggggag
     1261 tcccgccggc ggctgttcaa tgtcgcgggc gaagcggcct caggcgacat cgtgtgggaa
     1321 tggcgcggcg ccgatctgtt cgggaggacg gtctggcatt tgcgccgctg caacaacctg
     1381 ttgtatggca catataccaa ctatacatcc ggtggtgcga tcgagtccca cggcagcggt
     1441 gtgtggatca aatccacctt cagcagctct ttcgagcaat agccccggtt tgcactttac
     1501 ggcgcgccgg ttcgtgctag actgaaacgg tcaggaacca gcccatctat ccagaggcgt
     1561 gtcccaggga ctgttttcaa ggtgcgaagg gaggcagtca tgcgggagca aaaactgaag
     1621 gagctgctgg accgggaagg catcaggtac gtcaccatca gccattccac ggcctacact
     1681 gctcaggagg tggcggcgtc ggctcacgtt tcgggcaggg aaatggccaa gaccgtcatg
     1741 gtgagactgg acgatgagat ggcgatggtc gtcctgcccg ccagccgcaa gctcgatttc
     1801 gccaggctga aagagagcct gggcgcgcac aacgtcgaac ttgctgccga ggacgagttt
     1861 cgcggcttgt ttcccggatg cgacgcgggg gcgatgccgc cgttcggcaa tctctacggc
     1921 gtgaaggtct acatcgacga gaacctgagc gccgaagacc gtattgcctt caacgcgggc
     1981 tcgcataccg aggtgatccg gatgccgtac cgggattttg cccggctggt caaccccaga
     2041 gtgctgtcca tcgcccgcta gactccggga gcgcccgctg cgcatttcac cggagcgggc
     2101 gcgggtctac cgtttgtctg ggggctggct cgcaagcagg gtgagcgctt cccgaactcg
     2161 cgtggcctgg atcgcatcag caataagata gcgatgattg ccgtcggggc tttcctcaaa
     2221 tgtggacaag cctttgtcat cgtagcgtat gcggccggga ttggacaggg tgaaatagcc
     2281 gcgctctggc cggacggccc acagcacgct tgtcagatcc caggtcgggc gctcgtgggg
     2341 cgtggggttg tacaactggt acgcctctgc cagcggatga tgtgcggcgt agctgaaatc
     2401 ctgctcgatg ctctccgcgg gataggtgat cgccaggcca atctcgtagc cgctgcacac
     2461 aatgggtgtc ggccagttgt ccaccagttt ttgcgcggca ggaatatcca tcaccacgtt
     2521 gtactccggg tgttcctcgc cgtggatcag ctcgaacgcg cccgccatga tggacaatac
     2581 gcggaccttt gccgccgcca ggtcgaggcc gttgagcggc gaaatctcgt ccggaggcgt
     2641 atcgagaagc gccgcgagat tggtcgagaa ccccacctgg acaatcgcga cggagtggtc
     2701 gggctgggcc gcgagcacct gccgcagcac cgtcacggcg tccggtgcat catttccgct
     2761 ctggaggtcg tgggggtagc gcaagacccc gttgtcttcc atgcgggtaa ggctgaggaa
     2821 tttgctgtcc tgtgttgtga tgccgctgtg cgtcactcca atggggatgc cgccgcgacc
     2881 gtaaaacgta ttgatcgcgt ctacgagcgg tgcgcacctt tcgtgatcct tggtgagggt
     2941 gacggcgagg agctcgcatt ccccccggct ttggagcgcg tggattacgc ccagggcgag
     3001 cgcgtcgtcg acgtcgttgc cgatgtcggt gtcaaagatg agtttgacgg gctcggccgg
     3061 cttcagattg acccgttccg ggggcatttc cgtcgccgcg ttccagatca tcgagagcac
     3121 acatgcgcac cgcaacatac gccacctcct tcgatatcag gcacgaatcc ggggattacg
     3181 cgacacacag gcgcctcgcg caagcctcaa acatactgtc ccgtggccgg cgaatcaagg
     3241 ttctccctga gataccggcg cgggcgcttc cacgacgggt tctttgacgg ggaaaaacac
     3301 tcgaaacgtg gtgccgctgc cgggagtact gtcgactttg atggcgccgt aatgtccacg
     3361 cacgatgccg agcacggcgg ccaggcccag gccgcgcccg gtaaatttgg tggtatagaa
     3421 cgggtcgaaa atgcgggaca cggcactttc gctcatgccg gcgccggtat cggtcacgtc
     3481 gagatagaca taatggccct cgggctggtt ttcatgcagc caggttgcgg cgaggtccgc
     3541 gtgggtgcag aataccgcgc ccgtggcgat cgtgatcaac ccgcccgtct gatcgatcgc
     3601 ttccgacgca ttaatggcga gattcatgat caattggctg atttgtgagg gatcgccctc
     3661 gaaaaccggc acgcgccggg ccaggttgaa acggagccgg caggtatctg caatcgacgt
     3721 ctggatcagc tgcgccattt ccatgatgaa cgtgttgagt tcaatgagct cgagcgccgc
     3781 cttgccacgg ccggagtagg ccagcatctg gcgggagagt tcgatggcct tgcgcgcggc
     3841 gccggcgatc tctttcagat acgggcgtgc gccggcggcc tgtgaaagcc ccgaaatggc
     3901 catctcggcg ttgcccgcaa tgatcgccag gatattgttg aagtcgtgtg cgatgccccc
     3961 ggctacagtt acgaggctct cgagcttctg cgcctggcga acctgcgcct cgagtgccag
     4021 ctgctgcgtc acgtcccgtt ttaccgacac gtagcccata agcgcgccac ggtaatcaaa
     4081 gatgggcgag atggtggcat cttcctgaat gagcgtcccg tcttttctgc ggttggtcag
     4141 ggttccctgc cagaccttac cctcgagcaa tgccgcccac atggtttcgt aatacgcctg
     4201 gctgtgcgcg ccgctcttga gaatgcgcgg attctggcct acggcttcat ttcgtgaata
     4261 tcccgtgatc cgttcaaagg cggggttgac gtacacgatg atgccctgag aatccgcgat
     4321 cacaatatct tccgccgcat gttcaatcgc agtgaacagg cgccgttgcc ccagctccgc
     4381 cagccggcga tcggtgacgt cccgcaaaat catgacgctg cccacagggg ttccccagcg
     4441 cgagcgaatg ggcgagagcg cggcctccag ataccgcgtc gcaccgtcat tcatctgcac
     4501 ctgggtctca acgtccttgc ccggatgcat tgatgcgtcg aagagcgcct gtgaaaacgt
     4561 taaaaagtcc gttaccggcc tccccaggat cgctgattcg cttttgccca gcatcgcccg
     4621 ggccgcttca ttcaggtcga ggatctgatg gcccaggtca aacacgatca cgccgtcttt
     4681 catgctttcg atgacctggt tcctggccac gggcaccggc ggcagcaaca agccatagcg
     4741 gaggaagttc cacccgatca cgagcgccgc gaatccaaac gacaacggcg tcaagtccat
     4801 gtggggaatc ggactgaaac caatctgaaa cacgatattg gcggcgagcg gcgtgagcgt
     4861 gccgagcagg aggaacagcg tctgcctgcg atagatgcgg tgcgaacgta ccgccgcacg
     4921 cacgagaatg atcgtgccgg cgagaatgaa cgtgtacgcg tacgccagca gcagccaata
     4981 cagcggtccc cgctccatgg ccagtgtcat gacaggcgcc cgggtttcaa gccgcagcga
     5041 actccagatg aggtggtgcc gttcgttcgt ccagaccagg gccacgttca cgatcgggaa
     5101 cacgatgaac gccttgaaat accagggcag gcggcggttg cgcccctgcg aaaactggaa
     5161 ggcgaaaaca agccaggcca gcgggatgac cgcgatcccg acgtggcgga tgcgactcca
     5221 gagcagccgg cccgcgatgg tctggcagcc cagctcggca ccttcgccga ccgtccacac
     5281 gcacgctgca atcatcagga tcatcagcgt gatcgcgccg tattgactgc gctggcgcca
     5341 gaggaccgcc gcaaatactg ccgcgatgcc ggacgtcgcc agcaacggta gagcaaaaag
     5401 atgaaactgc caaccgggca tcgacctgca atcctccacc agcccccgga caggagcctg
     5461 gtctatcacg ccctgcaaac cgtgcggccg cgccaacgtg cggtgcgcgc gcgctggcgc
     5521 cgtcaacgcg cgagcgcgtc atggacgcgc cgcagcaggg tctccgaacg aaaaggcttc
     5581 cgcagcaagt ccggctggcc ggccggcaga tggcctttgt ccagtacgtc aaaactgtat
     5641 ccactcatca acagaacgcg ggtattggaa aaccgcgcct ggatctccgt atacacatcg
     5701 ccgccacttc gccgcggcat gaccacatcg agaatcacca ggtcgatgtc gcgggcatgc
     5761 tcgatgatgg cagccagggc ctcatctccg tcgcgcgcga ggaggacacg gtatccggcg
     5821 cgtttcagga cacgttcagc aagattccgc acctgttcat cgtcttcggc cacgaggatg
     5881 gtctcgttat tcccccgctg cgcctcttca cttgtcccgt ggcgcagttc aagcgcctcc
     5941 cttccgggtt catacacggg aaaatacaca cggaacacag caccctggcc gggccggctc
     6001 tcgagatcca gaaatcccag atggcgcgtc acaatcgcgt acacggtggc gaggcccagc
     6061 cccgtgccct ccccaacgcc tttcgtggta aaaaatggtt cgaagagatg ctccacgata
     6121 tccgggtgaa ctcccgaccc cgaatcggcc acgctgagca ccacgtaacg gcccggctgg
     6181 gtctccatga aacgcgacgt aaaaccctcg tctatctcgc ggcgttcgac ttcgagctcc
     6241 agacgccccc cctggggcat ggcatcccgc gcattcacgc agagattcag gatcacctgc
     6301 tcaacctggc cctgatcggc gaagatcgtg acatcgcccg gtcccggctc aaacaccatt
     6361 tcgatgtgct cgccgatcag gcgccggatc atcttgatca gaccactcac cacctcattg
     6421 aggtccagcg gggtctgttc gagtttttcg ctccgcgcga aggtgagcaa ctgccgcacg
     6481 agggtggtgg cccgttcagc cgcgcggtcc acttcctcca ggccgctttg ttccggagac
     6541 ccctgcggga cttcattgac gatcagctcg atatttccgc ggatgacctg gagcaggttg
     6601 ttgaagtcgt gcgcgatacc tcccgcgagc tggccaacgg cctccatttt ctgtgcctgc
     6661 cgcagttggt cctccaggat ccgccgggcc atctcattct gtttgcgttc ggtgatgtcg
     6721 cgcaagatct cgataacggc gctgggccgg ccttccgtat catgggcggc gacaccggcc
     6781 gtgcaccaga aatggcgcag cttcccgtgt atatcgagga tctgggtttc actgtcgtgc
     6841 acctggcccg tgctgagcgc ccggaaaacc ggacaatgtt cgcagggaag ctgggtgcaa
     6901 tcgaaaactt cgcggcattc ttttccgacg agtgcctcgc cgaacgcggc tttcgcatgg
     6961 ccgttggccc acgtgacgat gcgctcgcgg ttcatcatca gcagcggatc ggtgatggag
     7021 ctgatgatcc cctgcgcacg ctcttcgctc gtgcgcacgg cctcgttggc ggtcgacagc
     7081 gcggcggtgc gctggctcac ggcgcgcgag agccggtagt tccagaagag gaccagcagc
     7141 aggagcgcca acacgccgag cgccagtttc cacaccaggg aaaacggaaa ccccgggtcg
     7201 taccggagtg atacccattt ctgatagatg gcgtggcgcc gtggcggagg gattgcggca
     7261 aacgccttgc gcaccgacga taccagcagc ggcgccgagg cgcgtatcgc gatgcgctgt
     7321 tcgtacacat acggcgtttc acccaccacc ttgatgttgg aaaatccctg ccggctgaga
     7381 taatgactcg ctgtgacgat gggggctgcg aacaccgaca cgtccttgcg ctgcagcata
     7441 tccagggctt ccacaaccga atccgcgtgt acgatcgtga tttgcgggtg ctcctgctgg
     7501 atgagagcct cgatggcatg actgcgcacg acgacgatgc gttgcccgta cagctcgcgc
     7561 aggctgccca cgtaggccag ctcatcgcgc gcgaagagca tgacgggaaa ggacaggtac
     7621 ggttccgtaa actcgaaatg gcgggtgcgc ttttccgtgc gcgcgatagc ggtgaagaga
     7681 tcgacttgcc ccgcgcggcc cagtttctca agatcctgcc agctgtggtt tcggagaaac
     7741 acgacctcga gaccgagcat ttccgcgatt tcgtgcagat aatcgatggc aattccctga
     7801 aattgaccgg tatcgtccag aaattcgatg ggagcccact ggctgtcgga agccacgtgg
     7861 atcacggggt tctcctgaag ccaggcccgc tcttcctcgg tgagcgccag ttggaacacg
     7921 gagtcgccgg gtgaaaagcg gggcagctcg atgccgagcc agcgcaactc gatcgcccgg
     7981 cgatcgctgt ccgtgatcga atccaccgcc ttgttcaata cagacagcat gacggcatcg
     8041 tcgttccgta tgccgatacg cgcatagccc acgggaggag ccgaatagac cagggccagc
     8101 ttcaggtttg tcagaaatcg cgctgaaatg gaatcgtaga gcacggcaag gtcgccaaaa
     8161 gcgccgccca ccttgccctc aatcagcagg tcgatgcctg aatcggtatc atcggtctcg
     8221 tggatcagca catcgggaaa gccttcccgc aacgcggcca gctgcgacga attgcgcact
     8281 gctgccacgg cgcgccccgc aaactcgtcg agggaagaaa ttgcgggttc gtcgcgccgc
     8341 gtgagcaggg cctgcgggta caccgcatac acgtcggtga aattgaggta ggggcgccgc
     8401 tcctccaaca ggtccgcgtt gacgacccca tctactttat ggagcagtgc tttgcccagc
     8461 gcctccggcc agggggcgcc ttccaggcgt atctcgatgc ccaggcgcgc ttcaagcagc
     8521 cgaacgtaat cgacgttgat gccggccagc tcgccgtgtt cgttccggta gctcttgggc
     8581 tggtgatcgt tgtcgatcga aaagacgagc acgggatgtt cgtcgagcca ggcccgttcc
     8641 tccggcgtaa gcgtatccag cagcggcggc acggtgggtt cgttcaccgc aaccgcggct
     8701 ccgctcacag cgctggcgga cgcgagcagg gcgcacagca cggccgttcc acccagcgct
     8761 gccggtaaaa gggcgtttct caggatgcgc atcagacgca tgaattcgag tctcttgctg
     8821 ccgaacatcg aaccaaaatt gacaccaccc ttcattatag ccttccagcc tgcgggcgca
     8881 accggcggcg gcgtgcggca gcatggcgaa tcgtccgggg atggcttctc gcgtccgccg
     8941 tgcttgcacg ggaaccgggc ctggccgtag actggccctt ggccgtgagc gttgttttgg
     9001 cgaggcaacg gagagccgca atgctgagtt tacactttct ggcgggagtc ttggcggctc
     9061 tggctgctgt cgtggcggcg gacgagccaa gctcgattga attgaacggg gccgtgtacg
     9121 gcgcgacggc cgacgaacgc ggccccatcg gcggagggcc cggatatgcg gccgctgtga
     9181 cgtcgggcga cctcacagca gacacgctcg agacgttaat tgaggcgctg gcaaatgccc
     9241 aggccgggca gaccgtattt gtgcccggct ccgccgaaat tgatttcacc acgtttattt
     9301 atattgacaa gttcgtgctc gaaattcctg aaggcgtcac gctggcgagc gatcggggcg
     9361 tcgccggctc gccgggcgcg ctccttttca gcgatacact cgaaacctgc ccgatgatcc
     9421 gcgtaaacgg gcccggagtc cgcgtttccg ggctgcggat ccgcgggccg aacacgaaac
     9481 gttatctcga ccaccatgat cgcgcattcg gtaaaggcgg gggcgggcac agctattact
     9541 acaaatttcc ggtccaggac ggtatcgcga cggtctacga cgggctggag gtcgataatt
     9601 gcgagatttc cggattcggc cactcggggg tatacctcac caaagggcag aaccaccaca
     9661 tccaccacaa ttacatccat cactgccaat acaacgggct gggatacggc atatcgcaca
     9721 acgaatcgtc gtcgctcatt gagcgcaatc tgtttgattg gaaccggcac tcgatcgccg
     9781 gctcagggcg ccccggcagc ggttacacgg cgtgccataa cgtggaactc ggcacatcgc
     9841 tcagccactg tttcgatatg cacggcgggc gcgaccgtaa agacggcacg gacatcgccg
     9901 gcacgcgcct ggacatccat aacaacacat ttcgcgcgcc caaaccgccc atcgtgatac
     9961 gcggaattcc cgaagaatcg tgcgaagtct accggaactg gttccccctg catgctccgg
    10021 gccaggcggt gaacgccggc gagcgcacta cggtgactga caatgcgtac ggggcggatc
    10081 ccacccaggg agaatgacgc gcccggaacc gccggccggt ccatcatttg ctgtgatcga
    10141 agaagaactg agcgatcagc cccgcgatgg gcggaaagaa aaacgtgctc gcaatcctcg
    10201 ccagcatcag ttgccagccc agtattccca cctccatggg cagccgtgcc acggcccaca
    10261 gcgaccacgc ggtcaggaac gccaccatgg tgccggtgct cgccccggag cgcaccagtg
    10321 cggccgcaat aggcaggctt acgtaggggc cgcccggcgc cagaccgccg gccaccgtgc
    10381 cgatggcaat gccgcgcagg cccgattccg ccccgaccca gcgggcaatg atttcggttg
    10441 gaaccagcgc ctgcaccatt cccgccacca cgaacgcgaa caagagcagc ggcagcacgg
    10501 taatggtcat aatgagggcg gatctcaggc cttcgaggtg ctggccttcg ccccgcatgt
    10561 acccggcaag cagcagaacc gcggccagga ctcccatgat tatcgtaggt gctaacatgg
    10621 cactttgccc ccgtttcgga cgatcggtca ttgacgtctc cccggagccg cgcggcccat
    10681 gcttacgcca gttgcgggac ttcgaatccc gagcgatagc ccgggcgcac gagcagcgca
    10741 ttggcttcgg catcgttccc gaacgtctcg gtctgcgggt caaacgtcac gtcacggccc
    10801 agccggaatg caatgtttcc gagatggcag aaggcggcgc tgacatggcc gtccgcgggt
    10861 tttgcggcca cggcatcgcg gttgcgcgag cggatcgcat catggaaatt cgcaagatgc
    10921 agaagcacgg cgtcgccgtt ccggggtgga ttctcgtcgg ggatctcttt cccttcggtg
    10981 tcgtaacagg tattgcccac agcatatccg tttgagccgt agaacaagtt gccgcttttc
    11041 aattcgttct catcattgga gtaccgcccg cgaatctcgc aggtgaacag cgttccgtcg
    11101 gcaaaggtgg ccgttgtcgt ctggacattg ggcgtttcgg cgctgtcctc gtacccgtag
    11161 cgcccgccct ggctcgcaat gtgcacggga agaccttttc caaggaccca attgacgatg
    11221 tccgcgtagt gcacgccgtt gttgccgagt tcgccgttgc cgaagtgcca gaaccagtgc
    11281 cagtcgtagt gcacgtattt cttcgagaac ggcttatcgg gtgcgggccc ttgccagagg
    11341 ggccagtcca aggtttccgg cggcgcctcg tcgggatgaa acggcacggg ggggcgcgtg
    11401 ctgtaatgaa tcacgcgggc ggcgtaaagg tcgccgatca cgccggcctg gagccgctcg
    11461 accagccgtt gagtcgacgg ttcggagcgg cgttgcgtac cgacctggac gacccgtccg
    11521 gtctgttccg cggcagcgat aagctggcgc ccttcccaga tggaatgcgc catcggtttt
    11581 tccacataga cgtcttttcc ggcctggcaa ccccagaggg ccgcgagcac gtgccaatgg
    11641 tttggcgccg ccacaaacac cgcatcaata tcgtcagcgg ccagcaactc gcgcagatca
    11701 cgatacgcgg ccaccgtgac at
//
LOCUS       DAOPBQ010000040        11687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_5421_length_11687_cov_4.505244, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000040 DAOPBQ010000000
VERSION     DAOPBQ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11687
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5421_length_11687_cov_4.505244"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            508..1314
                     /gene="cysE"
                     /locus_tag="PKW60_02475"
     CDS             508..1314
                     /gene="cysE"
                     /locus_tag="PKW60_02475"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HPJ98328.1"
                     /translation="MTAPKTDAIWEAIRNEAAEETRKEPLLANFLYGAVLNHKTFEDA
                     LSFILATKLESTTIHAVALRDLIDEALVHDPGIGAAARADIEAVRDRDPACRAYSNPL
                     LFFKGFHAIQSYRIANYYWRAGRESLALFLQGRISQVLAVDIHPAAQIGCGILMDHAT
                     GVVIGETAVVEDNVSMLHEVTLGGTGKETGDRHPKVRKGVLIAAGATILGNIEIGEGA
                     RIGAGSVVLDDIPPHTTAVGVPARVVGRPRSEQPALDMDHYIVAGFGDGI"
     gene            1427..2893
                     /locus_tag="PKW60_02480"
     CDS             1427..2893
                     /locus_tag="PKW60_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPJ98329.1"
                     /translation="MTRREFLVRAAGGMAGLALTGRGLGMAEEANRRPNFVFILADDL
                     GWSDLGAFGSTFYETPHCDRLATEGMRFTNAYAACPVCSPTRASILAGKYPARMATTD
                     WFGAPQPDSVDRHWTKDKPLLPAPYEEQLPLEEVTLAEALHEGGYRTFFAGKWHLGGE
                     GFFPEDQGFEINRGGHDRGSPPGGYFSPYKNPKLEDGPEGEYLPLRLASETVQFIEAH
                     RDEPFLAYLSFYSVHTPLETRSDLKAKYTQKAATIDPALPRWGKEGEREVRLVQDHAV
                     YAGMVEAMDTAVGTVLDALDRLGLAGNTVVIFMSDNGGLSTSEGHPTSNLPLRAGKGW
                     LYEGGIREPLIIRWPGVVKPGTQCDVPVTSTDFYPTMLEMAGLPWRSEQHVDGVSLMP
                     LLMGRGSLPPRSLYWHYPHYGNQGGSPGAAIRDGDYKLIEFFEDSRLELYDLRTDIGE
                     TRNLAAGMPEKVQILADKLHAWQRDVGARFPKPRGGNT"
     gene            complement(2967..3710)
                     /locus_tag="PKW60_02485"
     CDS             complement(2967..3710)
                     /locus_tag="PKW60_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPJ98330.1"
                     /translation="MKKLVGFALLALVAAALNAQAAPPEGEGWRSLFNGENLDGWDNK
                     SGHAEVWQVKDGVIDCNPRVDLPGDKNLWSKESFRDFQLYIEWRIKGTKGEYPVPTVL
                     PDGSHKLDENGNPIITMTPNADSGIYLRGSSKAQINIWCWPVGSGEVYGYRMDDKMSP
                     EVRAGVTPKMKADKPIGEWNSFVITMKGDRLTVELNGQTVIENAQMPGTPEEGPLALQ
                     HHGGYHKGDDQWNSASSLVQFRNIYIKPL"
     gene            3927..5318
                     /locus_tag="PKW60_02490"
     CDS             3927..5318
                     /locus_tag="PKW60_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPJ98331.1"
                     /translation="MMKDMTGKELLLAAMRNEETPRPAWLPFVGVHGGKLIGKTATDY
                     LQSPDAIVEGLTKARELYRPDGLPILFDLQMEAEVLGCQVQWSDEVPPSVISHPLTSC
                     TLDDLPEFHAGKGRYPIAKEALLRCKETMGGEVALYGLITGPFTLASHLRGSELFLDM
                     LTRPAEVHALVNYCAEVGCRAADFYLQNGADVIAVVDPMTSQISQAHFEDFIAQPLNR
                     VFAHVAANNGLSSLFVCGDATRNLELMCRTKCDNLQVDENIPLDLLKNLAGKYNKSFG
                     GNLKLTVVLLLGSTADCMLDAIRCIDLAGLRGFILAPGCDLPYATPEQNLIAVSQMVH
                     DDYQRQVSRTSLKATQMESFEDLQLPDYSNKDVVFVDVITLDSSACAPCLYMVDAARN
                     AAGQANCYTTVREHKVKTRNGIGYMCRLHVENIPSICIDGVVRFSSQIPDRETLIAAI
                     EARAREKQGGTAP"
     gene            5315..6229
                     /locus_tag="PKW60_02495"
     CDS             5315..6229
                     /locus_tag="PKW60_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HPJ98332.1"
                     /translation="MIALSLITGFLGSGKTTYLRRLIEQYRGRRLVFLVNEFSPLDVD
                     GPLLQDASGDVVSLPGGSIFCTCLVSQFVSTLKGIPERFGGDTPVEGVIIEASGIANP
                     NVAVRMLRETGLDAQYALRSIVSIVDPGTFPVLLETLPNLAAQIEASSTIIVNKVDLF
                     DEKAVEAVEEQIRRLNSGARVVRSTYCDVALDLLAMPGCRDLDGDYAPCADPNYARFT
                     ERVAAPLDETRLRAALTNAERVLYRVKGFVPGRTGSLYLDYSSSGLVLQPAGAGPRFH
                     ELVIIASAKAYAEARSFVESLKHGEFNA"
     gene            complement(6340..7725)
                     /locus_tag="PKW60_02500"
     CDS             complement(6340..7725)
                     /locus_tag="PKW60_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98333.1"
                     /translation="MIRARHQYTSPGGMNRRDFVRLGAAAGMGASIAAMDRSAFAAVP
                     EALGPAPEMPFAAPPIDNVRIGYVGVGGMGTSHLRNLLAIEGCQIVAVCDIVEEKVAN
                     AQKMVEDKGFPKPTGYSRGERDFERLCAEEDLDLVYTATPWEWHVPVCMSAMENGKHA
                     ATEVPAAYTIEDCWKLVEGAEKYQKHCVMMENCCYGRAEMQILNMVRQGHFGELVHGE
                     GGYLHDLRALKFADSGEGLWRRQHTIDRNGNLYPTHGLGPIAQCMNINRGDQFDYMVS
                     MSSQALGMKLFAEENFPEGDPRRAETFRNGDYNTSLIKTALGRTIMVQHNCDSPRPYS
                     RINMLQGTRAITVGYPDRIYIEGRSPAHTWEELEQYREEFDHPLWRQLGPDAGGAGHG
                     GMDFMEDYRLIKCLREGLPLDMDVYDAAAWSAVGPLSEMSVANRAETLDFPDFTRGRW
                     RTWQPLGIVEA"
     gene            7956..8618
                     /locus_tag="PKW60_02505"
     CDS             7956..8618
                     /locus_tag="PKW60_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HPJ98334.1"
                     /translation="MPHTKAALKDMYERMSRFYGPTDWWPGDTPFEIAVGAVLTQNTN
                     WKNVERAIANLKRERLLSPQKILACEDRVLEAALRPSGYFRVKARRLRSFCAYLVDRY
                     RGSMKRLAAQPLDALRTELLAVNGIGPETADDILLYACDKPVFVVDAYTRRIFSRHSL
                     CAPDIAYEPLRALFERNLEADLHLYKEYHGLIVFVGKDFCRRTPKCAECPLGVLLEQH
                     PA"
     gene            8726..11455
                     /locus_tag="PKW60_02510"
     CDS             8726..11455
                     /locus_tag="PKW60_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98335.1"
                     /translation="MKRMLAAVLGLLLGASNAMAAEAKAASVTWRELWRADAPVILPI
                     EASPAQTTLTFAQVELEDSESLCLRFRARLDAPQDAGWNNYLAISLNGAALDARMDTG
                     EPRSLNRRNSFETDNPRYPRVNLFEPRAGLPCLQAFFGPAEPHLEALLTDRGEGYWYL
                     LDISDIAQGWRNELTIVNAAIASYWNGSPPEGMQLILEDLSVGAVPAREIRDLRGAHL
                     LKRQPLSGITVPGEGDTVRIPSSGGLRIDIAGEPYYLETSFSYPKDDGMGFNELNCTG
                     RPAGEPGWKPETQIAAPNVTLRAEGASYRVERSIRRPQELKERPEELPDTSEGPWFER
                     TQRCILITDTITNTTDEVLGLAVRHRLITPDRPHQARLGGLDTASLGLGEFPENPAVY
                     AGQSRGGLGVVPEDNHMRLQQERVLQENALEFGTKRLGLKPGESYTLRLAVYPVEGDY
                     FDFINAVRRDWDVNFTIDGPFDFFDVRRLASEEGRQEAAAMIARKRLKCFALTPWFEY
                     YDGWDYSREQYKEMMTEAKAFIRSIVPKARCLACVETNLVPVPLSFFQGALPADFPIG
                     RDVGGRYGQKATPAMTALVDASIWGDSVLRDADGGVLLDCWYVQHYGAKPALNLMVYP
                     TLDNYRHKHFLEQAAFLLDDVGLDGLYIDQFSLAWSESPQRYTKSAWDGRTVVLDANG
                     RVSEQWMDLGLVSAAARRIWVQSVLDRGKTVVCNTLPALFELQNLRAFRFMETQGYDP
                     LAEGVPFMDKLAKGQLGSPIGLGHSFPADAGADFFMRTVAAHLRFGQLYYYYVTEFPP
                     RVADVGGGDEDKSDLEEKQVIHGRVAGEFGPVTAMFPFTPRELHEGWILGEERLITTV
                     SGEFPWPFNRTPKVRLFNRFGIETEATSHVKFRAGEYSISITLDDWHEIAVVE"
BASE COUNT         2204 a         3433 c         3787 g         2263 t
ORIGIN      
        1 ggggtgaatc ggcggtagcc gatttggggg tggagttttg caggttggat tttggggtgt
       61 ggcttgcagc tcacagcatc ccgtcgtaac gcgtaggcta tccggctggg cgatcacacg
      121 gggactgatt tccgcagcgc gtaggggcag acacaggggt ctgcccttct tcaacgcagc
      181 catggggact atccggatgg gcaggcacag gggcctgccc cagcgttata aagacttcag
      241 gagatagtcg atgagataaa caaccggcca aaaaaagcct cggatactga acaccccatg
      301 aaatcttcct tcagaaacga aaaatcgcgt tgcgtgcact tagcacttga atgtgagggc
      361 cgatggaaaa gcgcgtttat gaaattccgg cgcggggccg ggcgttgtct gggtaaaggg
      421 tctgcgcccg gcgccgccgg atttttcatt tgggaacatc ctggcataca ctgacgcggc
      481 aatcgggccg tacccggagg agtattcatg actgcgccaa agaccgatgc catctgggag
      541 gcgattcgga acgaagccgc cgaagagaca cgcaaggagc cgctgctcgc caattttttg
      601 tacggagcgg tgttaaacca taagacgttc gaggatgcgt tgagctttat ccttgcgacc
      661 aagctcgaaa gcaccaccat ccatgccgtg gcgcttcgcg acctcattga cgaggcgttg
      721 gtgcatgacc ccggcatcgg cgcagcggcg cgcgcggaca tcgaggccgt ccgcgaccgg
      781 gatcccgcct gccgggccta ttccaacccg ttgctgtttt tcaaagggtt tcacgccatt
      841 caatcctacc ggatcgcgaa ctattactgg cgcgcggggc gcgaatcgct ggccctgttt
      901 cttcaagggc gaatttccca ggtgctcgcg gtcgacatcc atcccgcggc acagatcggc
      961 tgcggcatcc tcatggacca tgccaccggc gtggtgatcg gcgagaccgc cgtcgtcgaa
     1021 gacaacgtct cgatgctgca tgaagtcacg ctgggcggta ccggaaaaga gaccggggac
     1081 cgccatccca aggtccgcaa aggcgtcctg atcgcggcgg gagccactat cctcggcaat
     1141 attgaaatcg gtgaaggggc ccgaatcggc gccggaagcg tggtgctcga cgacatcccc
     1201 ccccacacca cggctgtcgg tgtacccgcc cgagtcgtcg gccggcccag gtccgaacag
     1261 cctgccctcg acatggacca ttacatcgtc gccggcttcg gcgatggcat ttagttctgg
     1321 ggatacgctg ccaaaaatgt cctcttgatt gactgtgccg ctccgattcg atgatctggc
     1381 gcgcccaatg gagtatggta tccccataat ccggagggta aaggggatga cacgcagaga
     1441 atttctggtc cgtgccgctg gcggaatggc gggactggcg ttgacaggac ggggattggg
     1501 gatggcggaa gaggccaatc gccggcccaa tttcgtattc atcctcgccg acgatctggg
     1561 gtggagcgat ctgggggcgt tcggcagcac attttacgag acgccccact gtgatcggct
     1621 cgcgacggaa ggcatgcgct tcacaaacgc ctacgcggcc tgcccggtat gcagcccgac
     1681 ccgggcgagc attctggcgg ggaaataccc ggcgcggatg gcaacaacag actggtttgg
     1741 cgcaccgcag ccggattcgg tggacaggca ttggactaaa gacaagccgc tcttgcccgc
     1801 gccctatgaa gagcagcttc cgctcgagga ggtgacgctc gccgaggcgc tgcacgaggg
     1861 gggctatcgg acgttcttcg ccgggaaatg gcacctgggc ggggaagggt tttttcctga
     1921 ggaccaggga tttgagatca accggggcgg ccatgaccgg ggcagtcccc cagggggcta
     1981 tttctcgccg tataaaaatc cgaagctgga ggacggccct gagggcgaat atctgcccct
     2041 gcggctggca tccgagacag tccagtttat cgaggcgcac cgtgacgagc cgtttctcgc
     2101 gtatttgtcg ttctattcgg tgcacacccc gcttgagacg cgcagcgatc tgaaggcgaa
     2161 atatacacaa aaagcggcca ccatcgaccc ggctctcccc cgctggggaa aggaaggcga
     2221 gcgcgaggtc cggctggtac aggatcatgc cgtgtatgcg gggatggtcg aggcgatgga
     2281 tacagccgtg gggaccgtgc tggacgcgct ggatcgcctg ggactggctg gcaacaccgt
     2341 cgtgatcttc atgtctgata acggcggatt gagtacctcg gaggggcatc cgaccagcaa
     2401 cctgcccctt cgggccggga aaggctggct gtacgaggga ggaatccgcg agccgctgat
     2461 tatcaggtgg ccgggagtcg tgaaacccgg cacccaatgc gacgtgccgg tcacgagcac
     2521 ggatttctat ccaacaatgc tcgaaatggc cggcttgccg tggcgcagcg agcagcacgt
     2581 ggacggcgtg agcctgatgc cgctgttgat gggccggggc agcctgccac cccgctcgct
     2641 gtattggcat tatccgcatt acggcaacca ggggggcagc ccgggcgcag ccatccgcga
     2701 cggcgattac aagctgatcg agttctttga agatagccgc cttgaactat acgacctgcg
     2761 cacggatatc ggagaaacca ggaacctcgc cgccggtatg cccgagaagg tgcaaatcct
     2821 ggcggacaag cttcacgcct ggcaacggga tgtgggcgcg cggtttccga aaccccgagg
     2881 cggcaatacg taacacggcc cgccacgccg tcgtgcgagg ctgcgccctt cggccaggga
     2941 tttagttcgg ctttgtcccc gcaaaatcag agtggtttga tgtagatgtt gcggaactgg
     3001 acgaggctgg atgccgagtt ccactgatcg tcgcctttgt ggtatccgcc gtggtgctgg
     3061 agggcgagcg gcccctcttc gggggtgccg ggcatctggg cgttctcgat gacggtctgg
     3121 ccgttgagct cgacggtgag acggtcgccc ttcatggtga tgacgaagct gttccactca
     3181 ccgatgggct tgtcggcctt cattttggga gtgacgccgg cgcgcacttc aggggacatc
     3241 ttgtcgtcca tgcggtagcc gtagacttca ccggagccga cgggccagca ccagatgttg
     3301 atctgcgctt tggaggagcc gcgcagatag atgcccgaat cggcgttggg cgtcatggtg
     3361 atgatggggt tgccgttttc atcgagtttg tggctgccat cgggcaggac cgtgggaacg
     3421 gggtattcac ctttggtgcc tttgatgcgc cattcaatgt agagctgaaa atcgcggaac
     3481 gactcttttg accacagatt tttgtcgccg ggaaggtcga cgcgggggtt gcagtcgatc
     3541 accccgtctt tgacctgcca gacctccgca tgaccggatt tgttgtccca gccgtcgagg
     3601 ttttcgccgt tgaagagcga gcgccagcct tcgccttcag gcggagcggc ctgggcgttg
     3661 agagcggcgg caacgagggc gagcagggca aatccaacga gttttttcat ggtgtaaaca
     3721 ctcctctgcg tccgcctgga aaaggctggc agtctgtgat tttcgtgaca tgcggactcg
     3781 taaggcattg tagagtcggg ggccgtctat ttcaaccgga gcagcgatcc attttcatgc
     3841 ggtctgtggt ataagggcct gtccgtgtgg aatgcacggg tggtacccgg cggcagaggc
     3901 aatgccgtgc ctgtgggagc gtggttatga tgaaagatat gacggggaaa gagctgctcc
     3961 tggcggcgat gcggaacgag gagacgcccc ggccggcctg gctgccgttt gtgggagtcc
     4021 atggcggaaa actgatcgga aaaaccgcaa cggactacct gcagtccccg gacgccattg
     4081 tcgaagggtt gacgaaggcc cgggaactct accggcccga tgggctgccg atcctgttcg
     4141 atttgcagat ggaagcggag gtgctgggct gccaggtgca atggtccgac gaagtgccgc
     4201 cgtcggtgat ctcgcatccg ttgacgtcgt gtacgctgga tgatctgccg gaatttcatg
     4261 ccgggaaggg gcggtacccc atcgcaaaag aggccctgct ccgctgcaaa gagacgatgg
     4321 gcggggaagt ggcgctgtac gggctgatca cgggtccgtt cacgctggca tcacacctgc
     4381 gcggcagcga gttgtttctg gacatgctga cccggcccgc cgaggtgcac gcgcttgtga
     4441 attattgcgc ggaggttggc tgccgggccg cggacttcta tctccagaac ggcgcggatg
     4501 tgatcgccgt ggtggatccg atgacgagcc aaatctcgca ggcgcatttc gaagatttta
     4561 tcgcgcagcc tctgaacaga gtgtttgcgc atgtggccgc gaacaacggg ctgtcgtcgc
     4621 tgtttgtgtg cggcgatgcg acgcggaacc tcgagttgat gtgccggacg aaatgcgaca
     4681 acctccaggt cgatgagaac attccgctgg acctgcttaa aaatctggcg gggaaataca
     4741 acaaatcgtt tggcggcaac ctgaagctca cggtggtctt gctgctgggg agcacggcgg
     4801 attgcatgct tgacgccatc cggtgcatcg atctggcggg actgcgcggg tttatcctcg
     4861 ccccgggctg cgacctgccc tacgccaccc cggagcaaaa cctcatcgcg gtgagccaga
     4921 tggtgcacga cgactatcag cggcaggtgt cgcgcacgtc gctcaaagcg acgcagatgg
     4981 agtcgtttga agatttgcag ctgcccgatt acagcaataa agacgtggtg tttgtggacg
     5041 tgatcaccct ggactcgtcg gcctgcgcac cgtgcctgta tatggtggat gccgcgcgga
     5101 acgccgccgg tcaggccaac tgctacacga cggtgcggga gcacaaggta aaaacccgaa
     5161 acggcatcgg atacatgtgc cggctgcatg tcgagaacat accctcgatc tgcatcgacg
     5221 gcgtggtgcg cttctcgtcg cagatccctg accgggagac gctgatcgcc gccatcgagg
     5281 cgcgtgcgcg agagaaacag ggaggcacgg cgccgtgatc gcgctgagtc tgatcacagg
     5341 ctttctgggc agcggcaaga ccacgtacct gcgccgtttg atcgagcaat accgcgggcg
     5401 gcgtctggta tttctcgtaa atgagttttc tccgctggat gtcgatggtc cgctgttgca
     5461 ggacgccagc ggcgacgtcg tctcccttcc tggcgggagc attttttgta cctgcctggt
     5521 aagccagttc gtcagcacgc tgaaaggcat tcccgagcgc tttggcgggg acaccccggt
     5581 ggaaggggtc attatcgaag caagcggcat tgcgaacccc aacgtggcag tacgcatgct
     5641 tcgcgagacc gggctggatg ctcagtacgc cttgcgttcc atcgtgagca tcgtggatcc
     5701 gggcacattt cctgtgcttc tggagaccct tcccaatctc gctgcgcaaa tcgaggcaag
     5761 cagcacgatc atcgtcaaca aggtggatct ctttgatgag aaagcggtgg aggcggtgga
     5821 agagcagatt cgacgcctca actccggcgc acgggtggtc cggtcgacgt attgcgatgt
     5881 ggcgctggat ctgctggcca tgccgggatg ccgggacctg gacggagact atgcgccgtg
     5941 cgcggacccg aattacgcgc ggtttaccga gcgcgtggcg gccccgctcg acgaaacccg
     6001 gttgcgcgca gccctgacga acgcggaacg ggtgttgtac cgggttaagg ggttcgtgcc
     6061 ggggcgaacg ggcagtctct atctggatta ttccagctcg ggtctggtat tgcagcctgc
     6121 aggcgctggg ccgcgcttcc acgagctggt tatcattgcc agcgcaaagg cttacgccga
     6181 agcccggagc ttcgtcgaat cgctgaaaca cggcgagttc aacgcctgaa tcgtcctgtg
     6241 cgcgccagag cttcggatca caaaaagagc ccgcaggacg cgaagaccct gcgggcccgg
     6301 tacatcctcc cggtacgggt tcgtcagcgc ggcggacgtt tacgcctcga cgattcccag
     6361 cggctgccag gtgcgccagc ggccccgggt aaagtccgga aaatcgagcg tttcggcccg
     6421 gttggctacg gacatctcgc tcagaggccc cacagcgctc catgcggctg cgtcgtagac
     6481 gtccatatcc agcggcagtc cttcgcgcag acatttgatc aggcggtagt cttccatgaa
     6541 gtccatgccg ccatggcccg cgccgcccgc atcgggaccg agttgcctcc agagcgggtg
     6601 atcaaattct tcgcggtatt gctcgagctc ttcccaggta tgggccgggc tgcggccttc
     6661 gatgtaaatg cggtcggggt agcccacggt gatggcccgg gtgccttgaa gcatgttgat
     6721 gcggctgtag gggcggggcg agtcgcaatt gtgctgcacc atgatcgtac ggccgagggc
     6781 ggtcttgatc aggctggtgt tgtaatcgcc gttgcggaag gtttcggcgc ggcgcggatc
     6841 gccttccggg aagttctcct cggcgaagag cttcatgccc agcgcctgag agctcatcga
     6901 gaccatgtaa tcaaactggt cgccgcggtt gatgttcatg cactgggcga tcgggccgag
     6961 cccgtgggta ggatagaggt tgccgttgcg atcgatggta tgctggcggc gccacaggcc
     7021 ttcgcccgaa tccgcgaatt tgagtgcgcg caggtcgtgc aggtagccgc cttcgccatg
     7081 gaccagttcg ccgaagtggc cctgccgcac catgttcagg atctgcatct cggcccggcc
     7141 gtagcagcaa ttctccatca tgacgcagtg cttctgatat ttctccgcgc cttcgaccag
     7201 tttccagcag tcctcaatgg tataggcggc gggtacttcg gtggcggcgt gcttgccgtt
     7261 ttccatggcg gacatgcaca cgggcacgtg ccactcccac ggcgtagccg tgtacaccag
     7321 gtccaggtct tcctcagcgc acaggcgttc aaaatcgcgt tccccgcgcg aataccccgt
     7381 gggcttggga aatcccttgt cttcgaccat cttctgcgca ttggccacct tttcctcaac
     7441 gatgtcgcac accgcgacga tctggcagcc ttcgatggcg agcaggtttc ggagatggct
     7501 ggtccccata ccgccgactc cgacgtagcc gatgcggacg ttgtcgatgg gcggtgccgc
     7561 aaacggcatt tccggcgccg gacctagggc ttccggaacc gcggcgaagg cggagcgatc
     7621 cattgcagcg atgctggcgc ccatgcccgc ggccgcaccc agtcgaacga aatcgcggcg
     7681 gttcatgccg ccgggtgaag tgtactgatg ccgtgctcgt atcatgtccg gttcctttcc
     7741 tgagttgcat gacgggaaac gaaattctaa gcgaagcgcc gggaaaatac cagtgaacaa
     7801 attgcccctc gccccggctt gtgtgcattc ggcgggtcag aggcccgggg tgcgttcgag
     7861 aggcgtggcg ccgggcagct tttgtgggcg cgtgcgggaa tgtgtcagta taggcgccga
     7921 aaacctttgg atacgccccc tgcaggaatc ttccgatgcc gcatacaaaa gctgccctga
     7981 aagacatgta cgagcgaatg agccggttct acggccccac ggactggtgg ccgggcgaca
     8041 cgccgttcga gatcgccgtg ggcgctgtac tcacccagaa cacgaactgg aaaaacgtag
     8101 aacgggcgat cgcgaatctg aaacgcgagc gcctgctcag cccgcagaag atcctggcct
     8161 gcgaagacag agttctcgag gcggcgctgc gcccgtccgg ctatttccgg gtcaaagccc
     8221 gccgcctgcg cagcttttgt gcctatctgg tggatcggta ccggggaagc atgaaacggc
     8281 tggccgcgca gccgctggac gccctgcgga ccgaattact ggccgtgaac ggcatcggcc
     8341 cggaaaccgc cgatgatatt ctgctgtacg cgtgtgacaa gcccgtgttt gtcgtggacg
     8401 cctatacccg gcgcattttc agccgccaca gcctctgcgc accggatatc gcctacgagc
     8461 cgctgcgcgc gctgtttgaa cgaaatctgg aagcggatct gcacctgtat aaggaatatc
     8521 acggactgat cgtctttgtg gggaaagatt tttgccggcg cacgccgaaa tgcgccgaat
     8581 gcccgctggg ggtgctgctg gaacagcatc cggcctgagg cgcggcgcat ccttgccccg
     8641 taacgcgggg cttcgttatg ctgtctgcgc gagtgaacca gccggatttt gtggcaaatc
     8701 gcagtgagaa gggagaggtc tgtccatgaa acgcatgctg gcggcggtgt tgggtcttct
     8761 gctgggggca tcaaatgcga tggcggcgga ggccaaagca gcgtcagtga cgtggcggga
     8821 gctgtggcgc gcggatgcgc ccgtaattct gcctattgaa gcctccccgg cccaaaccac
     8881 cctgactttt gctcaggtgg agctggagga cagcgaatcg ctctgtctgc gattccgtgc
     8941 gcgcctcgat gccccgcagg acgcaggatg gaacaattat ctggcgattt cgctgaatgg
     9001 cgccgccctg gacgccagga tggacacggg cgagccgcgc agcctgaatc gccggaactc
     9061 cttcgagaca gacaaccccc gctacccgcg ggtcaacctg tttgagcccc gggccggcct
     9121 gccgtgcctg caggcctttt tcggcccggc ggagccgcat ctcgaggccc tgctcacgga
     9181 tcgtggcgaa gggtactggt atctgctgga tatcagcgac atcgcgcagg gttggcgcaa
     9241 tgaattgacg atcgtcaacg cggccatcgc atcctactgg aacggttcgc ccccggaggg
     9301 catgcagctc atccttgagg acctgagcgt gggcgccgtg cctgcacggg agatccgaga
     9361 cttgcgcggg gcgcacctgc tcaaacggca gccgttgtcg ggcatcacag ttccaggcga
     9421 aggcgacacg gtgcgcattc cctcgagcgg cggcctccgc atcgacatcg ctggcgaacc
     9481 gtattatctc gagacttcat tttcatatcc caaggatgac ggtatgggct tcaatgagct
     9541 gaactgcacc ggcaggcctg ccggggagcc cggctggaag cccgagacgc agatcgcggc
     9601 ccccaacgtg accctgaggg ctgaaggtgc gtcctaccgc gtggaacgga gcatccggcg
     9661 cccgcaggaa ctcaaggaac gcccggaaga actaccggat accagcgaag gcccgtggtt
     9721 tgagcggact cagcgctgca tcctcatcac ggacacgatc accaacacga cggacgaggt
     9781 gctgggattg gcggtgcgcc atcgcctcat cacgccggac cgtcctcatc aggcacggct
     9841 gggcgggctg gatacggcga gcctcggcct gggcgaattt ccagagaatc cggccgtgta
     9901 cgcaggccag agtcgtggcg gcctgggcgt cgtgcctgaa gacaatcaca tgcgcctgca
     9961 acaggagcgc gtgctgcagg aaaatgcgct cgaattcggc accaaacgcc tcggcctcaa
    10021 accaggcgaa tcgtatacgc tccgcctggc cgtctatccg gtcgagggcg attacttcga
    10081 cttcatcaac gccgtgcgca gggactggga cgtcaatttc acgattgacg gcccgtttga
    10141 tttcttcgat gtgcggcggc ttgcatcgga agaaggacgc caggaagccg ccgcgatgat
    10201 cgcgcgcaaa cgccttaaat gcttcgcact gaccccctgg tttgagtatt acgacggctg
    10261 ggactactcg cgtgaacagt acaaggaaat gatgaccgag gcgaaggcgt tcatccgcag
    10321 catcgtgccg aaagcgcgtt gtctggcctg cgtcgaaacc aacctcgtgc cggtgccgct
    10381 gtcgtttttc cagggcgccc tgcctgcgga tttccccata ggccgcgatg tgggcgggcg
    10441 atacggccag aaggccacac ccgcgatgac ggcgcttgtg gatgcatcga tctggggcga
    10501 ttcggtactg cgcgacgccg acggcggtgt gctgctcgat tgctggtatg tgcagcatta
    10561 cggcgcaaaa cccgccctga atctcatggt ctatcccacg ctcgacaact accgccacaa
    10621 gcacttcctg gaacaggccg cctttctgct cgacgacgtg ggccttgacg ggctctacat
    10681 cgaccagttc agcctcgcct ggagcgaaag ccctcagcgc tacaccaaat ccgcctggga
    10741 tggccgcacc gtcgtgctgg atgccaacgg ccgcgtctcg gagcaatgga tggatctggg
    10801 cctggtttcc gccgcggcgc ggcgaatctg ggtgcaaagc gtcctggatc gcggaaagac
    10861 ggtggtctgc aacacgctgc cggccctctt tgagctgcag aacctgcgag cgtttcgttt
    10921 catggaaacg caggggtatg accccctcgc cgagggcgtg ccgttcatgg acaaactcgc
    10981 caaagggcag ctgggctcac cgatcgggct cggccattca ttcccagccg atgccggggc
    11041 cgatttcttc atgcggacgg ttgccgcgca cctccgcttc gggcagctgt attattacta
    11101 cgtcacggaa ttccctccgc gcgtcgcgga tgttggtggc ggcgatgagg acaaatccga
    11161 tctcgaggaa aaacaggtca tccacgggcg cgttgcgggc gagttcgggc cggtgaccgc
    11221 gatgtttccg tttaccccgc gggaattgca tgaaggctgg attcttgggg aagaacggct
    11281 cattacaacg gtatcgggcg agttcccctg gcccttcaac cggactccaa aagtgcggtt
    11341 gtttaaccga ttcggaattg aaaccgaggc cacatcccac gtcaaattcc gcgcgggcga
    11401 gtattccatc tccatcaccc tggatgactg gcacgaaatc gcggtcgtag aataaaacag
    11461 cgcgcgcaac cgcctgggtg cgccgccgtc ctcgtcggca cacggttcac ccgcgcccgc
    11521 ggaggatagg cactctgggt gcgccgccgt cctcgtcggc acacggttca cccccgcccg
    11581 cggaggatag gtactctggg tgcgccgacg tcctcgtcgg cacacggttc acccgcgacc
    11641 gcggaggata ggtactctgg gtgcgccgac gtcttcgtcg gcacacg
//
LOCUS       DAOPBQ010000041        11541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_5502_length_11541_cov_3.944019, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000041 DAOPBQ010000000
VERSION     DAOPBQ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5502_length_11541_cov_3.944019"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1081
                     /gene="mdh"
                     /locus_tag="PKW60_02515"
     CDS             131..1081
                     /gene="mdh"
                     /locus_tag="PKW60_02515"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPJ98336.1"
                     /translation="MPVGKRFKIACIGAGNVGATVAQYCLDMQLGDVVLFDILEGLPQ
                     GKGLDLTQAGPVRGYSCTATGTNDYRDIAGSNICVVTAGLPRKPGMSRDDLVEKNGTI
                     VAGICENILRYAPESVVIFVTNPLDVMAYLALKKLGYPPRKVVGMAGTLDSARLRTFV
                     ASELGVSMKNVDTMVLGSHGDEMVPLPAYTTVSGVPITQLLPEDKIEAIVERTRKGGG
                     EIVALLKTGSAYYAPAASVARMVQCILNDERQILPCSAFLTGQYGLKDVFIGVPVRLG
                     EGGVEEIIELELTPAQMEALHKSADAVREGIQSLKNFGLL"
     gene            1473..2363
                     /locus_tag="PKW60_02520"
     CDS             1473..2363
                     /locus_tag="PKW60_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98337.1"
                     /translation="MDRSQKLALAVFIVGTVVGVAIIVISGTGLSSVQQAPFRAAPGK
                     QLGSARVFVTETAQEAAVPAHPGESEAKAPAHGVHPRPAADEPGPFTPSPKTLSPLDQ
                     AIWEARHAYEPWTGVDRIRELLRALENLGKASELYAAQAELYLHMDPPDLANAQRAAS
                     EAVNYASTAEERDRARWAEAEVAQRSGEVERAWELASGLASEEGPVSEGKLRAALLTA
                     TIARNRDDSAAAEAAYRGAMQRAVASGNWSDPHCTDVYRQAAWNLVHLLRESDRRDEA
                     DAVAEEAARQLELSHTRSLE"
     gene            complement(2479..3873)
                     /locus_tag="PKW60_02525"
     CDS             complement(2479..3873)
                     /locus_tag="PKW60_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98338.1"
                     /translation="MIHLLTAAAFALSTGAFDLVSIPLKNPEAQWFAVAIDDGAALEM
                     AVLDQYTLTIYNIDTGARRFSVTFPEGVSAFDILDVDGDGLAEIIALQPPRVLQIDLE
                     GAAAPRELFRDDDLLEQAGAFPAPHVLATRRDGSWLLALPGRSALRFWTLGGELADTV
                     PLASPGLNPAPFERPFTARTVYPNLVAPDGAIELRIDQTVVYEAASAVAAPAGRAETV
                     RRPGSLVHLLNAEDDALHRWRWFSLVRPGEEQRRVLYAPGGSEYEDTLIRVEQAGAAS
                     GAPGRESQVSPARRYRGCLIPPGETLPDFNGDGYTDILVWLSPQPGRSIDALTHAAVA
                     RSWPLQLLVYCFDPEKNRYDPRNAGVIDTHVPIAWFMRPGQGSPLNLLVMGDFDGDGR
                     SDLAFATAPNTFCVWLYDNGFPRKPSFEHTFPEGLLQLESPGDIDGTGRTAAVLRTPG
                     TLYILRPPAIRHPE"
     gene            complement(3870..5369)
                     /locus_tag="PKW60_02530"
     CDS             complement(3870..5369)
                     /locus_tag="PKW60_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPJ98339.1"
                     /translation="MIPVLAWLVCGALVFVEQDLPGVRVGRAEPLSGERWRNFRQADF
                     DGDGELDLLLPDCVAFQRNGAFRSEDRHALPSVSGLPACDLWNTALYMRLPAGLACFQ
                     WSSDGWQTLFEHPIRWPDREALSQGMDSVPGGDAASLRFERFLHDFNQDGRPEIVVAA
                     EDGLHVFMMDGERCIPAGVFDVFPPLRLIPENPARVWPPDQRRIPVSESQLSCRLFIT
                     ENRVTVLESDILSENRARYRMECFEIAAAPGGHLEFKPLGRQETPALPSTLRPCRLNP
                     DETIDFAGGQWQFASGNLLHAPVYEMCVSTDFGQSIQSVRSISYRPSCSFVDFNGDGR
                     LDLVAESSGLFEGGVRETVSRLMTARALKHHIEIHLQDDNGTFPAAPTIRGSFHLTID
                     QVPARSDERFRRYQASELIDITGDFDGDGIRDAVIHASPREIQVYRGTAAGFSSRPFL
                     TAAIEPHWRFAVDDIDGDGRSDIVFRWVDPASSGGLEHCRVLLAREEAR"
     gene            complement(5366..6160)
                     /locus_tag="PKW60_02535"
     CDS             complement(5366..6160)
                     /locus_tag="PKW60_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPJ98340.1"
                     /translation="MMRRIISAYVVEMQKAVCQRATYAGPLLVLLAVFAAMLQSPLER
                     DGLSDYRFVYDATTIALGVLGLFLIVIFCSGLIASELGRGSIRLLLVRPLRRVEFLLA
                     KLLLGATFAVVLLLVAGTASWALAALLGEVHGVEYGGETLYTSAGMAGTYAAAAAFSL
                     FPLLALVAFALLMSTLTRSSAAAIGWSIGLWLLLDLVKNPLGIQPYVFTTYLEPWQVF
                     AQRSDGFPEMGWMPGNPVYLVGVSVLWLVLLVALAAAVLQRRSLHA"
     gene            complement(6157..7056)
                     /locus_tag="PKW60_02540"
     CDS             complement(6157..7056)
                     /locus_tag="PKW60_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98341.1"
                     /translation="MSTCIETSDLTKCYGPVVAVGGLSLQIEAGEVFGLLGPNGAGKS
                     TTLYMLTGLVRPTSGAVTIFGKDLRHGFLEIAARMGVLVERPTFYDHMTARANLLLLA
                     RLAQRRVAVDRALDRVGLLWAAHQKVQGFSHGMRQRLGLAQALLTEPELLILDEPTNG
                     LDAESTRDTIGLLRLLAEQAGVTIIVSSNLLHEAEILCDRVAIMNRGRLLACERTEKL
                     LSYDPKQVELLVDAPETAAKRLMEQPWVNAVDLAYGRVNVKLGEASIHHLTSFLISSG
                     FIVSGIVPQRRSLQDFFLKVLKQ"
     gene            complement(7090..8817)
                     /gene="recJ"
                     /locus_tag="PKW60_02545"
     CDS             complement(7090..8817)
                     /gene="recJ"
                     /locus_tag="PKW60_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPJ98342.1"
                     /translation="MNSGKTALAWSCASCDRTAVRSLARELDIPPLVAQLLIIRGAAT
                     PELGHQFLNPSADHLCDPFELSGIGEAVERIRRARDLGEHVLVFGDYDVDGITGTAIL
                     YNALKAYGISSCSYDMPNRLEEGYGISPERVYQARDQEVGLIVTVDNGVTAREAAEAA
                     RAAGIGFIITDHHEPESQLPHADAIIDPKCAGPEHPCRDICGAAVAFKLATALTGRIN
                     DLDLVALGTVADIVPLHGENRALVALGLREIAARRRPGLLKLAQVARVNLETLTARNI
                     AFQLAPRINAGGRLGDGIAGMDLILTDSPQTAETIARELDRANSERRDIESALLDDAE
                     AELRETFTDQQRSIVLARYDWHQGVIGIVASRLQRRFYRPVALIALDGGGVGRGSIRG
                     IPEFDVSTALNACSHLLVKFGGHRAAGGLTIAEDNVQAFQCAFEEEAARQLDGRELRR
                     SLAIDAVIAFTQIDGQLLRWLDLLEPYGHGNAEPVFCTFNAEIVPQSCRYLKGGHVRL
                     VLQSDGRQFPAIGFHMGGLYDDLCTISRVDAAFTPQLNTYRDETTIQLELKDIRPARG
                     GDGSSGDSD"
     gene            complement(8814..9491)
                     /gene="cmk"
                     /locus_tag="PKW60_02550"
     CDS             complement(8814..9491)
                     /gene="cmk"
                     /locus_tag="PKW60_02550"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPJ98343.1"
                     /translation="MCNQITDIVAIDGPVGAGKSSVGRQVAERLGFAYLDTGAMYRAA
                     TWWAIENGTDLDNPEALTAITQNMPLHLSDSNGELRVRVGAQDITEVIRTPEITRQIY
                     KLDHAAGVREHLVRLQREFGARQPTVADGRDMGSVVFPKARWKIYLDASLEERARRRA
                     AQFAEKGLQVDLAELTREIEERDRKDMTRAVSPLQKAPDARVLDTTQLTFDQSVDAIV
                     DFVREVL"
     gene            complement(9572..>11541)
                     /locus_tag="PKW60_02555"
     CDS             complement(9572..>11541)
                     /locus_tag="PKW60_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98344.1"
                     /translation="GISLEDAALGRTWCDVRRYATNEPALQQAVLKRIDAWLKLYTRP
                     PCGTEGRRSQWLLRDLIDCVRVGEVSALERDELDFLSAAFHLANRMEDREASARMRTL
                     LIPYVNEIEIRRKILPSANGLAALSKRLHIIDTADIETAPYSAIRTIAGIEMPARGPL
                     LKLSGHVKLFGSVQDDCTLVVENGCCSVLGYVLGRLAATQHCDVRHNISGVAISSLGS
                     IRARNIIDQASVIAKAGNVQVRRVENPRLLFAGERLVVADSVRRGNIIAPDISVQNEV
                     LGGHLQVSRSLTADCFRATPELPLDITFRKRLANSDFGGTPTAYATRLWSQTLHLKTR
                     ILTLEALLAATDREIERLSENALFFVCSNESTEKHIDSIRKNKRRLDLIDRIIEGLLG
                     MAYAIAERAAERALALQESGEACLDEAGVMFSVADALLTQLGTDAAGERDVYACINSM
                     ADIRENLRRGVQGNRLATASLTRIREELRRWLDEKQLLTEQIAAADAGLHARLDFSEE
                     LQSAAKGSSPEQLLRHLIATARNQPAGGELAATLASNFVSAMLRSIDKKRQATEPLRR
                     ELSEKRGEFDERSKQLRTQHHLVVSLEEIDHGEGPRVSGRFEGGVVLYADALAAGQEQ
                     PPDTTRFVVPDTGEAVKTYQRRFTSIVETCL"
BASE COUNT         2277 a         3419 c         3856 g         1989 t
ORIGIN      
        1 ggccggagag tggctaccgg gcccccgcgg tagagttttc ggcgtttccg gcatatatta
       61 gggcggccag gtgttgggca gatgatgccg aaatcgcgaa tggcggacac aacccggccg
      121 gaggagcagt atgccagttg gaaagcggtt caaaatcgcc tgtatcggcg ccgggaacgt
      181 cggagcgaca gtggcgcagt actgtctgga catgcagctg ggcgatgtgg tgctcttcga
      241 cattctggaa gggctgcccc aggggaaagg gctggatctg acgcaggcgg gcccggtgcg
      301 gggatacagc tgcaccgcga caggcacgaa cgactaccgg gacattgcgg ggagcaatat
      361 ctgcgtggtg acggccggac tgccgcgcaa gcccggcatg agccgcgacg atctcgtcga
      421 aaagaacggc acaatcgtcg cggggatctg cgaaaacatc ttgcgctacg cgcctgaaag
      481 cgtggtgatc ttcgttacga acccgctgga cgtaatggct tacctcgcgc tcaagaaact
      541 cggctaccct ccgagaaaag tggtaggcat ggccggcacc ctggacagcg cccgactgcg
      601 cacattcgtt gccagtgagc tgggcgtgag catgaagaac gttgacacga tggttttggg
      661 ctcgcacggc gacgagatgg tcccgttgcc tgcctacacg acggtctccg gtgtgccgat
      721 cacccagctg ctgcccgagg acaagatcga agcgatcgtg gagcggacgc gtaaaggggg
      781 cggggaaatc gtggcgctgc tgaagaccgg aagcgcgtat tacgctcccg cagccagtgt
      841 ggcgcgcatg gtgcagtgca tcctgaacga cgagcggcag atcctgccct gctcggcgtt
      901 tctcacgggc cagtacgggc tgaaggacgt gtttatcggc gtgccggtgc ggctgggaga
      961 aggcggcgtg gaggagatca tcgaactgga actcacgcca gcacagatgg aagcgctcca
     1021 caaatccgcc gacgccgtgc gcgaaggtat tcagagcctc aagaatttcg gcttgttgta
     1081 gcgggtccga tatacggagc aggcgcgctg agacggaaaa gcgccgctgc cgagtccggg
     1141 cggtccttga gggaccgaaa atgtgaacgc ccggagaccc tcaaccacat tcaggtcgac
     1201 actacgcccc tcgaactgaa gccacaggtc tcaaaagaag ccacccccct ggcatctccc
     1261 catcgaactc gcctgccccg cgttggacag aaatggacag taaggccccc agcgggggac
     1321 gcagtgaagc gataatcaca gggaaacaca atgaagcaac gaccagcgaa ggacagctgg
     1381 cccggtgagc acaagggcgg agaagaaagg accgagcaat cagcgcttgg tgctctttac
     1441 ttgggaggca agacggggta tcgtaaaacc caatggacag gtcgcaaaag ctggctctgg
     1501 cggtgttcat cgtgggcact gtggtgggcg tggccattat cgtcatttcc ggcaccgggc
     1561 tttccagcgt gcagcaggcg ccgtttcgag ccgcgccggg aaaacagctg gggtcggcgc
     1621 gcgtcttcgt cacggagacc gcccaggaag ccgcggtacc ggcgcatccg ggcgagtctg
     1681 aagcgaaggc gcccgcgcat ggcgtgcacc ctcgtcctgc cgccgatgaa cctgggccgt
     1741 tcacaccgtc gccaaaaacg ctcagccctc ttgatcaggc aatctgggaa gcccggcacg
     1801 catacgaacc gtggacgggc gtggatcgca ttcgagagtt gctgcgcgcc ttggaaaatc
     1861 tgggaaaggc gtccgagctt tatgcggctc aggcggaact ctatctgcac atggatcctc
     1921 ccgatcttgc aaacgcccaa cgggcggcct ctgaagccgt gaactatgca tccaccgcgg
     1981 aggagcgcga ccgggcgcgc tgggccgagg cggaggtggc tcagcgctcg ggcgaggttg
     2041 aacgggcctg ggaactcgcg tcggggcttg catcggagga aggcccggtg tcggagggca
     2101 aactccgggc ggcacttctc acggccacca tcgcgcggaa tagagatgac agcgccgcgg
     2161 cggaagcggc ataccgtggc gccatgcagc gggctgtcgc ctcgggaaat tggagcgatc
     2221 cccattgcac agacgtatac cggcaggcgg catggaacct ggtgcacctg ctgcgtgaat
     2281 cggaccgccg cgacgaggct gacgccgttg ccgaagaagc agcgcgccag ttggagcttt
     2341 cccacacgcg ttcgctggag tgaaccgggc cctcggcacc ctgtcagctc aaggccccgc
     2401 gcccgcacac cgtgcatccc ttgagtgttc gataattcag gcagcaggca tgcggctcaa
     2461 tagcgcgagc gcgggatttc attccggatg gcggattgca gggggtctga ggatgtacag
     2521 ggtacctggc gtgcggagaa cggctgcggt gcgccctgtg ccgtcgatat ctcccgggga
     2581 ttccagctga agaaggccct cggggaatgt gtgctcgaat gaaggtttcc gggggaaccc
     2641 gttatcgtag agccaaacgc agaacgtgtt gggagcagtg gcaaaagcga ggtccgaacg
     2701 gccgtcgccg tcgaaatcgc ccatcaccaa caggttcagc ggggacccct gcccgggacg
     2761 catgaaccag gcgatgggca cgtgggtgtc gatgacgccg gcgttgcgcg ggtcgtaacg
     2821 gtttttttca gggtcgaaac agtatacgag cagctgtagc ggccaggagc gcgcgaccgc
     2881 cgcgtgggtc agcgcatcaa tggaccttcc gggttgaggg gagagccaca cgagaatgtc
     2941 tgtgtacccg tcgccattga aatcgggtaa ggtctctccg ggcggaataa ggcatcctcg
     3001 atagcgccgg gcggggctga cctggctctc tcgaccaggg gcgccgctgg ccgcgccggc
     3061 ctgttccacg cgaatgagcg tatcctcgta ttccgagccg ccgggcgcat acagcacacg
     3121 ccgctgctct tcgccggggc gaacgagaga aaaccagcgc caccggtgga gcgcgtcgtc
     3181 ttccgcattg agcagatgaa ccaggctccc tgggcggcga acggtttcag cgcggcctgc
     3241 gggggcagca acggcggatg cggcctcgta gaccacggtc tgatcgatgc ggagctcaat
     3301 ggcgccgtcg ggcgcaacca ggttcgggta gaccgtcctt gccgtgaacg gccgttcgaa
     3361 gggcgccgga ttcaggcccg ggctcgcgag cggcaccgtg tcagcgagct cgccgccaag
     3421 cgtccagaat ctgagggcag accggcccgg gagggcaagc agccaggagc cgtcgcgccg
     3481 cgtggcaagg acatgcggcg ctggaaacgc gccagcctgt tcaagcagat catcgtcgcg
     3541 gaacagttcg cggggggcgg ccgcaccctc cagatcgatc tgcagaaccc ggggaggctg
     3601 cagggcgata atctccgcga gcccgtcgcc atccacatcc aggatatcga atgcagagac
     3661 gccttccggg aacgtcacgg aaaaccgccg ggctccggtg tcgatgttgt agatagtcag
     3721 ggtgtactgg tcgaggacgg ccatttcgag ggcggcgccg tcgtcgatag cgacagcaaa
     3781 ccactgcgct tcgggatttt tcaaggggat ggagaccagg tcaaacgccc cggtgctcag
     3841 cgcaaacgcg gccgctgtca ggaggtgaat catcgggcct cctcccgcgc cagcaacact
     3901 cggcaatgct ctaatccgcc gctgctggcg gggtccaccc agcggaagac gatgtcgctg
     3961 cgcccgtcgc cgtcgatgtc atcgacggcg aagcgccaat gaggctcgat cgcggcagtc
     4021 aggaagggcc gggaactgaa tccggccgcg gtgccgcgat acacctggat ctcccggggg
     4081 ctggcatgaa tgaccgcatc acggatgccg tccccatcaa aatctcccgt gatatcgatc
     4141 aactctgacg cttgatagcg ccggaaacgc tcgtctgaac gagcgggcac ctggtcgatg
     4201 gtgaggtgga acgagccgcg gatggttggg gcggcaggaa acgtgccgtt gtcgtcctgg
     4261 agatggatct cgatgtggtg tttgagagcg cgcgcggtca tgagccggct tacagtctcg
     4321 cgcacgccgc cttcaaaaag gccggacgat tcagcaacca ggtcgaggcg cccatcgcca
     4381 ttgaagtcaa caaaagagca gctggggcgg taagagatgc ttcggaccga ttgaatcgat
     4441 tggccgaagt ctgtggagac acacatttcg tatacgggag cgtgcaggag gtttccggag
     4501 gcgaactgcc actgtccgcc agcaaaatcg atggtctcgt ccggattgag gcggcagggg
     4561 cgcaacgtac tggggagagc aggagtctcc tgcctgccga gcggcttgaa ttcgaggtgt
     4621 ccacccggag cagcggcgat ctcaaaacat tccatgcggt accgggcgcg gttttcggaa
     4681 aggatatcgg attcgagcac agttacccgg ttttcagtga tgaacagacg gcacgacaac
     4741 tgcgattcgg atacgggtat gcgccgctgg tccggcggcc agacgcgcgc gggattttca
     4801 gggatcaggc gaagaggcgg aaagacatcg aagactccgg ctgggatgca gcgctcgccg
     4861 tccatcatga agacgtggag gccgtcttcg gcggcgacaa caatctcggg gcggccgtcc
     4921 tggttgaaat cgtgaaggaa gcgctcgaaa cgcagcgacg ccgcgtcgcc gccgggcact
     4981 gaatccatac cctgggacaa ggcctcacgg tctggccagc ggatgggatg ctcgaacagg
     5041 gtctgccagc cgtcagaaga ccattgaaag caggccaggc cggccggcaa ccgcatatat
     5101 aatgcggtgt tccacaggtc gcacgcgggc agaccgctga cggacggcag cgcgtggcga
     5161 tcctcgcttc gaaaggcccc gttgcgctga aaggcgacgc aatcgggcag aagaagatcg
     5221 agttcgccgt cgccgtcgaa atcggcctgg cgaaaattgc gccagcgctc tccagagagc
     5281 ggttccgcac gcccaacccg cacgcccggc aggtcctgct cgacaaagac cagggcgccg
     5341 cagacaagcc atgcgagcac cggaatcatg cgtggaggct cctccgctgg agaaccgctg
     5401 ctgcgagcgc gacaagcagg accagccaca acacggaaac gcccaccagg tacacggggt
     5461 tgccgggcat ccatcccatt tcgggaaaac cgtcactgcg ctgggcgaat acctgccagg
     5521 gttcgagata ggtggtaaac acgtacggct gaatgcccag cgggtttttg accagatcga
     5581 gcagcagcca gagcccgatc gaccatccga tcgcggcagc cgagctccgc gtgagtgtag
     5641 acatcagaag agcgaaagcg accagagcca ggagcggaaa caggctgaac gcggccgcgg
     5701 cggcataggt tccggccatg ccggcgctcg tgtacagggt ctctccgcca tactcgacgc
     5761 catgcacctc ccccaggagg gcagccaggg cccagctggc cgttccggcg accagcagga
     5821 ggaccacggc gaacgtggct cccagaagca gcttggccag caaaaattcg acgcggcgca
     5881 gcgggcggac gagcaggagc cggatgctgc cgcggccaag ctccgatgcg atcagccccg
     5941 agcagaagat gacgatcaga aaaagtccca gcacgcccag agcgatggtc gttgcatcgt
     6001 acacaaaccg gtaatcgctg agaccgtcgc gctcgagggg gctctgcagc atggccgcga
     6061 aaacggccag cagcacaagc aaagggccgg cataggtggc ccgctggcag accgccttct
     6121 gcatctccac aacataggca cttatgattc ggcgcatcat tgtttgagca ctttcagaaa
     6181 aaaatcctgg agcgatcgcc gctgcggcac gatacccgac acaatgaacc cggaactgat
     6241 cagaaacgac gtgagatgat ggatggaggc ttcgcccagc ttgacattca cacgtccgta
     6301 cgcgagatcc acggcattga cccacggctg ctccatgagg cgtttggccg cggtttcggg
     6361 cgcgtcgacg aggagttcga cctgtttggg gtcgtaggac aagagttttt cggtgcgttc
     6421 acacgccaga agacggccgc gattcatgat ggcgacgcga tcgcagagga tctcggcttc
     6481 gtgaaggagg ttgctggaga cgatgatggt taccccggcc tgctccgcga ggagcctcag
     6541 gagtccgatg gtgtcgcgcg tggattcggc gtcgagtccg ttggtaggct catcgagaat
     6601 gagcaattcg ggttcggtca gcagggcctg ggcaagaccc agccgctgcc gcatgccatg
     6661 agagaagccc tggactttct gatgggcggc ccagagcaga ccgacccggt cgagggcgcg
     6721 atcgacggca acgcggcgct gggccagccg tgccagaagc aacaagttgg cccgcgcggt
     6781 catgtggtcg taaaacgtgg gccgctcgac gaggacaccc atacgggcgg cgatctcgag
     6841 aaatccgtgg cgaaggtctt tgccaaaaat ggtgactgcg ccgctggtgg gccgcacaag
     6901 tccggtcagc atgtagaggg tggtgctttt gccggcgcca ttcgggccga gcagcccgaa
     6961 aacctcgccg gcctcgatct ggagggacag gcctccgacc gcgaccacgg ggccgtaaca
     7021 tttggttaag tctgacgttt cgatgcatgt tgacatgcag aaaatcctag cacgtttcgt
     7081 ccggataaat cagtccgagt ccccggaaga cccgtcgccg ccccgggcgg gccggatgtc
     7141 tttgagctcc agctggatgg tggtctcgtc acggtaggtg ttgagctggg gagtgaatgc
     7201 cgcatccacg cgggaaatgg tgcataagtc atcgtacagg ccgcccatgt ggaacccgat
     7261 agccggaaac tggcggccat cactctggag caccagtctg acgtgcccgc ccttcaggta
     7321 gcggcaggat tgagggacga tctcggcatt gaatgtgcag aaaacaggtt ccgcattccc
     7381 gtggccatac ggttcgagca agtcaagcca gcggagaagc tgcccgtcga tctgggtgaa
     7441 cgcgatcacg gcgtcgatgg cgaggctgcg gcgcagttcg cggccatcga gctggcgggc
     7501 cgcttcctct tcaaaggcgc actgaaacgc ctggacattg tcctcggcaa tggtgagtcc
     7561 gccggcggcc cggtggccgc cgaacttgac gaggagatgg ctgcaggcgt tcagcgctgt
     7621 ggagacgtcg aattcgggga tgccgcgaat agagccacgc ccgacaccgc cgccgtcgag
     7681 ggcgatcagg gccacggggc ggtaaaaacg gcgctgaagc cgcgaagcga cgatgccaat
     7741 gacgccctgg tgccagtcat agcgcgcgag cacgatgctt cgctgctggt cggtgaaggt
     7801 ttcgcgcagc tcggcctcgg cgtcgtcgag gagcgcggat tcgatgtcgc ggcgctcgga
     7861 attggcgcgg tcgagttccc gcgcaatggt ttcggccgtt tgaggagaat ccgtcaggat
     7921 gaggtccatt cccgcaatgc cgtcgccgag ccggccgccg gcattgatgc gcggagcgag
     7981 ctgaaacgcg atgtttcggg cggtgagcgt ttcgagattg acgcgcgcca cctgagcgag
     8041 cttgaggagg ccgggccggc gcctggcggc gatttcacgc aggccgaggg cgaccagggc
     8101 gcgattttca ccatgcagcg gcacgatatc ggcaaccgtc ccgagcgcca ccaggtcgag
     8161 gtcgttgatc cgccccgtca gggcggtcgc cagtttgaag gcgacggcgg cgccgcaaat
     8221 gtcgcggcag gggtgctccg ggccggcgca cttcggatcg atgatggcgt cggcatgggg
     8281 aagctggctt tcaggctcat ggtggtcggt aatgatgaac ccaatgccgg cggcacgggc
     8341 agcctcggcc gcttcccggg cggttacgcc attgtctacg gtgacaatga gaccgacctc
     8401 ctggtctcgg gcctgatata cccgttcggg actgatgccg tagccttctt ccaggcggtt
     8461 gggcatgtcg tagctgcacg acgaaatgcc atatgccttg agtgcattgt agaggatagc
     8521 tgttccggtg atgccgtcaa cgtcgtagtc gccgaacacg agcacgtgtt cgccaaggtc
     8581 ccgtgcgcga cgaatgcgct caacagcctc gccgataccg ctcagttcga aaggatcgca
     8641 caggtggtcg gcggaagggt tcagaaactg atggccgagc tcaggtgtcg cggcgccgcg
     8701 gatgatcagg agctgcgcca cgagcggcgg gatatccagc tcccgggcca gtgaacgcac
     8761 ggccgtgcgg tcgcacgaag cgcaggacca tgccaaagcc gtctttcctg aattcacaat
     8821 acctcccgga caaaatcgac gattgcgtcg acggattggt caaacgtgag ctgcgtggtg
     8881 tcgagcacgc gggcatcagg ggctttttgc agtggcgaga cggcccgggt catgtctttc
     8941 cgatcacgct cctcgatttc tctcgtcagc tcagcgagat cgacctgcaa tcccttttcg
     9001 gcgaattggg cggcacgccg ccgggcgcgc tcttcgagcg aggcatccag atagatcttc
     9061 cagcgcgctt tcggaaagac cacggacccc atgtcgcgcc cgtcggcgac ggtgggctgc
     9121 cgggcgccga attcgcgctg gagcctcacg agatgttcgc gaacgcccgc ggcgtggtcg
     9181 agcttgtaga tctgccgcgt gatttcaggg gtgcggataa cctccgtgat gtcctgcgcc
     9241 cccacacgca cgcggagctc accgttgctg tcgctcaggt gcaggggcat gttttgagtg
     9301 atggctgtca gcgcctcggg attgtcgaga tccgtgccgt tttcgatggc ccaccaggtc
     9361 gcggcccggt acatggcccc cgtgtcgagg taggcaaatc cgagccgctc ggcgacctgg
     9421 cgtcctacgg agcttttgcc tgcgcctacg ggcccgtcaa tagccacgat atcggtgatc
     9481 tgattgcaca ccgggttcca aatcctctcc agcgatcctg cgcggagccg ctcactttcc
     9541 catcaacgga ttcaggcaag cctaacacgg actacaaaca ggtctcaacg atagacgtaa
     9601 aacggcgctg ataggtcttc acggcctcgc ccgtgtcggg cacaacgaaa cgggtggtat
     9661 ccggcggctg ctcctgcccg gcagcgagcg catcggcata cagaacaaca cccccctcga
     9721 atctcccgga cacgcgaggc ccttctccgt ggtcgatctc ctccaatgag acgacgagat
     9781 gatgttgtgt acggagttgc ttgcttctct cgtcaaattc cccgcgtttt tcggacagtt
     9841 cgcgtctgag gggctcggtg gcctggcgct tcttgtcgat gctgcgcagc atggcgctga
     9901 caaaattgga agcaagcgtt gccgcgagtt ctcctcccgc cggctggttc cgggcggtgg
     9961 ctatcaggtg ccgcaacagc tgttcggggg agctgccctt cgcggcggac tgcagctctt
    10021 cactgaaatc cagccgggca tgcagcccgg cgtcggccgc ggcaatctgt tcggtgagca
    10081 gttgtttttc gtcgagccag cggcgcaatt cctcgcgaat gcgcgtgagc gaggccgtgg
    10141 caagacggtt tccctgcacg ccccggcgca ggttttcacg gatatccgcc atgctgttga
    10201 tgcaggcata gacatcgcgt tctcccgcgg cgtcggtacc aagttgagtc agcagcgcgt
    10261 cggctacgct gaacatgacg ccggcctcgt cgaggcaggc ctcaccggac tcctggagcg
    10321 ccagggcacg ttccgcggcg cgttcggcaa tggcataggc catgccgagg agaccttcga
    10381 tgatgcgatc gatgaggtca agccgccgct tgtttttccg tatggaatcg atgtgttttt
    10441 cagtggattc gttgctgcag acgaaaaaaa gggcattttc ggaaagccgt tcaatttccc
    10501 ggtcggtggc agcaagcagc gcctcaaggg tcaggatgcg cgtcttcagg tgcagtgtct
    10561 gagaccagag ccgcgtggca taggcggtgg gggttccgcc aaaatcgctg tttgccagcc
    10621 gtttccggaa ggtaatgtcg agcggcagtt ccggagtggc ccggaaacag tccgcggtca
    10681 ggctgcgtga aacctgcaaa tgaccgccca gaacctcgtt ttggacggag atgtccgggg
    10741 cgatgatgtt cccccggcgg acggagtccg ccaccaccag gcgttccccc gcaaaaagca
    10801 gtctcgggtt ttcgacccgc cgcacctgca cgttgccggc tttggcgata acgctggcct
    10861 ggtcaatgat attgcgcgca cggatgcttc ccaggcttga gatggcgacc cccgagatgt
    10921 tgtggcgcac atcgcagtgc tgggtagccg caaggcggcc caggacatag cccagcacgc
    10981 tgcaacagcc attctcgacg accagagtgc agtcatcctg gacgctcccg aagagtttga
    11041 cgtgcccgga cagcttgagg aggggtccgc gtgcgggcat ctcaatgccc gcaatggtgc
    11101 gaatcgcgga atatggagct gtttcgatgt ccgccgtgtc gattatgtga aggcgcttcg
    11161 aaagcgcagc aagtccgttg gcgctcggga gtatcttgcg ccggatttcg atctcgttca
    11221 cgtacgggat aagaagggtc cgcatgcggg cactggcctc ccggtcctcc atgcggtttg
    11281 caagatgaaa ggccgcggaa aggaagtcca gttcgtcccg ttcgagcgcc gagacttcgc
    11341 cgacgcgcac gcagtcgata aggtcgcgaa gcagccattg agaccgccgc ccttcggtgc
    11401 cgcagggcgg acgggtatag agtttcagcc aggcatcgat ccgtttgaga accgcctgct
    11461 gcagcgcggg ctcgttggtg gcgtacctgc gaacatcgca ccaggtgcgg cccagcgctg
    11521 catcctcgag ggagatgccg t
//
LOCUS       DAOPBQ010000042        11357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_5617_length_11357_cov_3.973545, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000042 DAOPBQ010000000
VERSION     DAOPBQ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5617_length_11357_cov_3.973545"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PKW60_02560"
     CDS             <1..1011
                     /locus_tag="PKW60_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPJ98345.1"
                     /translation="FMSSGASFSAPYIFGNRYPMLSVEIFNERTQFNDARAATLTVLL
                     AGLSLLGVLVLRSRRKTGGGGSKGVRVPLRSRTGRLLATLAATLCMAVVLLPHLTILW
                     LSFVDHRAWHTELVPATFTWANYRAIFSSAQGFLPIRNSLWMSALAAGACLLVALPAG
                     YLIGRGRPGALWVNFLVMVPWALPGTVTAMNLILAFNHPRFSLVGTVWLLPLAYFIRN
                     VPLLTRISTAAIEPFDATLLEAGQALGASRWFCFRRIILPLMLPALAAGTALVFATCL
                     GEFVSSILLYAPGNVPIAVQIDTMRRGAAGLGSAFAYSVLLMVLVAGTFVLSRRFTSR
                     VF"
     gene            1142..3016
                     /locus_tag="PKW60_02565"
     CDS             1142..3016
                     /locus_tag="PKW60_02565"
                     /inference="COORDINATES: protein motif:HMM:NF028511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPJ98346.1"
                     /translation="MVSLRVKFGLFIVLVFGLVTAAMSLLQAHWVRTLVFEQTETRME
                     QNIHAAWNILRTRQQLLEAHVSALAELGELEEQSEAGTERLEEILQLYRERWGLDFVA
                     LTDTAGRVVMSDGALQDGAALPLFGETAAPSGSGRVRSGYAVAQPEVFSAPEIQARLV
                     SDGGPQNAMVLMAQYRLDASWAHEGDWLVAGTVLNGAHQLVDAIHHTIFPSEFYKGKP
                     LGTATIFLGPVRIATTVSLDNGKRALGTIVSDEVAEKVLKRGESWTGPALVVGHWYLS
                     RYEPIRTPDGAVLGMLYIGELRQIYDDIESRTLLTNLTVLFAIMMGALALSFVLISRV
                     LRQVVALDRATKGFVEGDYSARANIETRDELGGLAQSFNRMAATIQGDRAKIMRQNEE
                     IESANRGYMGMLGFATHEFRNSVGAAMLNLQLLKEGSFGKIEGEVFEGIDIVEKSLRY
                     ISDISDNFLQLSRIERGELSVTQARVDLNKSVVIPVLQDKKGIIELRKMTLETDIPDG
                     FSLTADPNLIRVALENLVGNAIKYGREGARIVIAARMVGNWAEVSVWNDGPPIPPDQL
                     NSLFEKFWRCDSDTVSSRRGAGLGLFLVKHIVDLHAGQVAVESSEEKGTRFILRFPGS
                     "
     gene            3292..3588
                     /locus_tag="PKW60_02570"
     CDS             3292..3588
                     /locus_tag="PKW60_02570"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HPJ98347.1"
                     /translation="MVGINGIGGIPEPLPDRPANVRDRKREVENASVREDGVQISSEA
                     REAASSARLASLVRGGDEVRAEQVAAAKERIERGDFKLPEVVAEVARRLSKYLT"
     gene            3675..4013
                     /locus_tag="PKW60_02575"
     CDS             3675..4013
                     /locus_tag="PKW60_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPJ98348.1"
                     /translation="MWPFGGALPGVQDGIASWYGNEYHGRLTASGEVFDQEAMTAAHR
                     EYPFGTRVLVTNLKNRRSVIVRINDRGPFIRGRIIDLSRGAARELNMLRDGIVPVRVE
                     VLEWGSGKYR"
     gene            complement(4173..5546)
                     /locus_tag="PKW60_02580"
     CDS             complement(4173..5546)
                     /locus_tag="PKW60_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98349.1"
                     /translation="MKQCTARENMSRRTFLASAAALSAASCATAPHIVTSAAAAKTPA
                     NERLNLGAIGFGGMGAVNIRNLSGEQFVALCDVDSAYAKPVFEAFPDARRYVDYREML
                     AAESGLDGVVIATPDHTHAVIAMAAIKAGKHVFCQKPLAHDVYECRMLAQAAAEAGVV
                     TQMGIQGHAGNGTRTVVESIQAGVIGEVREVHAFCSDSYYPWGHAGWSPKDGRVPEEA
                     MSVPETLNWDLWLGPAAARPYHSCYHPMAWRAFQAFGSGWMADRGAHSLDPVKWALNL
                     GHPSRISATSTGTTDEMHPVSAIVTFEFPKRGKMPPVSVTWYTGLHIPRPAGVPVGEQ
                     IGDPTGGILFVGSDGMMTCDTYGERPRLLPEARMKKFKAPEPSIPRIEGESIEGDWVR
                     AIRNGTKACADFSYSGPLSEICALGNIAKRFDDTPIEWDNDRMTVTNLDDANQYVRTP
                     YREGWSL"
     gene            complement(5701..7416)
                     /locus_tag="PKW60_02585"
     CDS             complement(5701..7416)
                     /locus_tag="PKW60_02585"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPJ98350.1"
                     /translation="MDKATASQKFQQSVQLFSQKHYVEALMLLDELNTAFPTQKQVMY
                     GRAQCLAELQRHAEAIAVCDQLIQWFDDDKARELKARIQQDNVSALDGPTDLASLLGA
                     GADSSNGGSGLPDLSGLLDLPPLPDPAAGAHRPARSLRKGLYTLLSCAGGLLVVLALG
                     FPVLASMRQEEKAAPQVGALAAAGDTGEAAAGPALSAEPRGCARPATIGGSGTAEIIG
                     EGEDALTLLRIQGNRYELGYWYGKLLADQIAATSKLAQALIEKSGIPAEMVNMATASL
                     WQEKNYDVAPWSTEVQGVADGCADAGHPELTFSVLQQMASIPDLSEYNCSLFAAWGAA
                     TLDGETYQMRNLDWSMDTGLQDYPVVAIYTPDDGVKHAVVGFAGVLGVSVGGMNTTGI
                     GVSEIMGHFCDSETLDGIPFPFLLRDVLYFDRDLQSALDRMKSAVRTNQYHYALADPN
                     AAGAKGRLLLTSHTRFDEYADNQSVNPHPCADLKPFHRPIEDALYWKNHNGRDNDVLY
                     NAILDRYGEIDAEKAIEIARIACVDGTLVSIIYHNSGNDMWVAFAEGMTPGPRQKYVH
                     VKLDD"
     gene            7955..9016
                     /gene="trpS"
                     /locus_tag="PKW60_02590"
     CDS             7955..9016
                     /gene="trpS"
                     /locus_tag="PKW60_02590"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPJ98351.1"
                     /translation="MAGTKEIILSGIRPTGRLHYGNYFGAIRNFIDLQSQDTICYYFI
                     ADYHALTTITEREDFYQQTIEMLRTYVACGLDPAKSVIYRQSDLPCTAELALLLGMIT
                     NIGFLARGTTYKDKMGKLEEGPGGVDGNPLSYGLLGYPVLMAADILIVRANKVPVGDD
                     QRQHLEMAIDIAQRFNSRFGETLTLPEGIARNALRLPGLDGGAKMGKSEHNTLDLLDD
                     AATVLKKIKAVPTTTDPEPLGTDSDHPDDVIPRMPNSVGALYRLLNLLAPEAVYLDFV
                     GKYRRREKFYGTLKTTLAEYVSAFNAPIIEQYNAPGNSREAVEEFLRENARKVTPVAL
                     DTVETVRAAMGIGRSLYRA"
     gene            9044..9985
                     /locus_tag="PKW60_02595"
     CDS             9044..9985
                     /locus_tag="PKW60_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPJ98352.1"
                     /translation="MHILIVGGTRFLGAAVARELVTRGHRVSLLHRGTTSGNVPPEAE
                     HLLGDARDRAFVESVLQPGRFDAVVDTILTSDDLQWYLPLLHKTVGQLVHCGSTGVYA
                     PMTTVPAREYDPTPCAPELGGFAEKLAQDRVLLEYHEHAGFQVCSLRVSNVFGAGDVP
                     LDIWGARNPQYFQRLADGEEIWLPGDGTTLLQPVHVDDLARGFRAAVEAPSRTTGQIF
                     NLSSDRAVTLTRYAELAAELLGSASMFRCVPMEEILATGRANEAGLRFICEHMCIDSS
                     KAGRVLHYEPKVSVREGLRDSLTWMIDTGRLRAERPA"
     gene            10062..>11357
                     /locus_tag="PKW60_02600"
     CDS             10062..>11357
                     /locus_tag="PKW60_02600"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPJ98353.1"
                     /translation="MKNRTLQVVYILCSTYDDEGYPLRFYRGILPSNTLNCLRSLTLD
                     VATSGALGDNVSVQVSIYDDTVEPIPVRRIARKLRRRNTELLVGLVGVQSNQFIRATD
                     LAMQFRDLGVQVVVGGFHVSGMLAMFDDVTPDLQRLLDLGVSLFQGEAEAPDALAGLL
                     RDALARELQPIYRQTAYPVLTDSGAPEPDLGYLKHFKCSSMGTLDTSRGCPFGCSFCT
                     VINVQGRRLRSRSAECILQSIRRSYERGINMWFFTDDNLARSPVWEELFDGLAAMRDE
                     GMDITFMMQVDTRAYAIPGFVEKAQRAGCYMVFVGMESVNPKNIEAMDKRQNDVEDYA
                     HMVEVWHQAEALVHVGYIIGLPDDTRESVRRDIETLMNEVKVDEASFFMLTPLPGSAD
                     HKRMVEECVPIDADLNNYDSLHETFRHPRMSPGEWTLAYK"
BASE COUNT         2092 a         3465 c         3579 g         2221 t
ORIGIN      
        1 ttcatgtcgt ccggcgcctc attcagcgcg ccctacatct tcggcaaccg gtaccccatg
       61 ctgagcgtcg aaatcttcaa cgagcgaacc cagttcaacg acgcccgcgc cgccacgctc
      121 accgtgcttc tcgcgggttt gtcgctgttg ggcgtgctgg tgctccgttc gcgccgcaaa
      181 accggggggg gtggttccaa aggcgtgcgc gtgcctctcc gatcgcgcac gggccgcctc
      241 cttgccacgc tggccgcgac gctgtgcatg gcggtggtcc tgctgcctca cctgaccatt
      301 ctgtggctgt cgttcgtgga ccaccgcgcc tggcataccg aactcgtgcc ggccacgttc
      361 acctgggcca actaccgcgc gatcttttcg tccgcgcagg gcttcctgcc gatccgcaac
      421 agtctttgga tgagtgcact cgctgcgggg gcctgcctgc tggtggcgct gcccgcggga
      481 tatctcatcg gacgcgggcg gccgggcgcg ttatgggtaa actttctggt gatggttccc
      541 tgggccttgc cgggcaccgt aacggcgatg aaccttatcc tggcgttcaa tcacccccgg
      601 ttctcgctgg tgggcacggt gtggctcctg cccctggcct atttcatccg gaatgtgcct
      661 ctattgaccc ggatctcgac agcggccatc gagccgtttg atgcgacgct gctcgaggcg
      721 gggcaggccc tgggcgcctc ccgatggttc tgtttcaggc gcatcattct gccactgatg
      781 cttcccgcgc tggccgccgg caccgccctc gtgttcgcca cctgcctggg cgagttcgtg
      841 tcgtccattc tgctctatgc tccaggaaat gttcccatcg ccgtgcagat cgacaccatg
      901 cggcgcggcg cggcagggct gggttccgca ttcgcgtaca gcgtcctgct gatggtcctc
      961 gtggccggca cttttgtctt gtcgcggcgg tttacgtcgc gtgtattctg atctgcggct
     1021 ggaagccgtg ccgcacgcgc cggaatggaa ccgggccgct gcgaggttgg aatttcgaag
     1081 gtgcatgcgc caccatatgg cgcgaacggg gcagtcatgg tgccgaaacc cagggagatg
     1141 gatggtgtcg ttgcgtgtga aattcgggct ttttattgtg ctcgtcttcg gactggtcac
     1201 ggcggcgatg tcgctgcttc aggcccattg ggttcgcacc ctcgtctttg agcagaccga
     1261 gacccgtatg gagcagaaca tccacgccgc gtggaatatc ctgcgcaccc gtcaacagct
     1321 cctggaagcg cacgtatccg cgctcgcgga actgggcgaa ctggaggaac agagcgaggc
     1381 gggcacggag cggctggagg aaatcctgca gctgtaccgc gaacgatggg gcctggattt
     1441 cgtggcgctt acggatacgg cgggccgggt cgtcatgagt gacggggcgc tgcaggacgg
     1501 cgcggcgctg cccttatttg gagaaacggc agccccttcc ggaagcgggc gggtccgttc
     1561 agggtatgcg gtggcgcagc ctgaggtgtt cagtgcgccg gagatacagg cccggctcgt
     1621 gtcagatggt ggccctcaga atgccatggt gctgatggcc cagtaccggc tggatgccag
     1681 ctgggctcac gaaggagact ggctggtggc gggcacggtc ctcaatggcg cccatcaact
     1741 ggtcgacgcc attcaccaca ccatttttcc cagcgaattc tacaaaggaa aacctctcgg
     1801 caccgcgacg atctttctgg ggcctgtgcg catcgccacc acggtgtcgc tcgacaatgg
     1861 aaaacgcgcg ctcggcacaa tagtcagtga cgaagtggcg gaaaaggtcc tgaagcgcgg
     1921 agaatcgtgg accggccccg cgctcgtggt cggccactgg tatctgtcgc gctacgagcc
     1981 catccgcacg ccggacgggg cggtgctcgg catgctctac atcggcgaac tgaggcaaat
     2041 ctatgacgac atcgaatcgc gcacgctcct gaccaacctg actgtgctct tcgccatcat
     2101 gatgggagcc ctggcgctct cgttcgtgct tatctcgcgc gtactgcgcc aggttgtcgc
     2161 gctggatcgg gcgacaaagg gttttgtgga aggagattac agcgcgcggg ccaacatcga
     2221 gacccgcgac gagctgggcg gtctggccca gtcatttaac cgtatggcgg cgaccatcca
     2281 gggcgaccgc gcgaaaatca tgcggcaaaa cgaggaaatc gagagcgcca accgggggta
     2341 tatgggaatg ctcggctttg ccacgcatga attccgcaat tcggtcggcg cggccatgtt
     2401 gaacctccag ctcctcaagg aagggtcgtt tgggaaaatc gagggcgagg tattcgaagg
     2461 tatcgacatc gtcgagaaaa gcctgcgcta tatcagcgac atcagcgata attttctcca
     2521 gctttcgcgc attgaacggg gcgaactcag cgtcacccag gcccgggtgg atctgaacaa
     2581 atcggtcgtg atccccgtgc ttcaggataa aaaggggatc atcgaactgc gtaaaatgac
     2641 gctggaaacc gatatccctg acggtttcag cctgaccgcg gacccgaacc tgatccgggt
     2701 agcactggaa aacctcgtcg gcaatgccat caagtacggc cgggaagggg cacgcatcgt
     2761 cattgccgcg cgcatggttg gcaattgggc ggaggtcagc gtctggaatg atggtccgcc
     2821 aatccctccc gaccagttga attccctgtt cgagaagttc tggcgctgcg attcggatac
     2881 ggtgtccagc cggcgcggcg ccgggctcgg cctgtttctg gtcaaacaca ttgtggacct
     2941 gcacgccggt caggtggcgg tggaatccag cgaagaaaag ggcacgcgct tcatcctgcg
     3001 cttcccgggc agctgaatcg cgcggagtca gactgcgtcc ggtttgggct cattaatttt
     3061 gtaaataggt tgtttttatt gacttacata tgctccgacc tgctttttcc gcttgaaaaa
     3121 ctggattaac aggctgccgc tgcttgcaaa cgggcctgga gtctggtatc acagcagcat
     3181 cttgacggcc cggcttcgcg gcaggggcta aagttttggt gcgcttgagc cgatgatcgg
     3241 tgtgtggcat ggcgctacaa catgaatcaa tacgtaacgt ggagagaacc catggttgga
     3301 atcaacggca ttggcggtat ccccgagccg cttcctgatc gcccagccaa cgtccgcgat
     3361 cgcaaacgcg aggtggaaaa tgcctctgtg cgcgaggacg gggttcagat cagctcagag
     3421 gcccgggaag cggcgagcag tgcgcgcctg gccagcctcg tccggggagg cgacgaagtt
     3481 cgcgccgagc aggtggcggc ggcgaaagaa cgaatcgagc ggggcgattt caagctgccg
     3541 gaagtggtgg cagaggtcgc gcgccggttg agcaagtact tgacataaaa ccaggatacc
     3601 agcgtatacg gacacggttt cacggcacga ggattctcgt gctttttgtt ttggcggggt
     3661 cggcaggctg cagaatgtgg ccgttcggcg gggcgcttcc gggtgtgcaa gacggcattg
     3721 cctcgtggta cggcaacgaa taccacggcc ggctcacggc ctccggtgaa gtgtttgacc
     3781 aggaagccat gacggccgcc caccgggaat atccgttcgg tacccgcgtt ctggtcacca
     3841 acctcaagaa tcgccggtct gttatcgtgc gcatcaacga tcggggccct tttatccgag
     3901 gccggatcat cgatctgtcc cgcggcgccg ccagagaatt aaatatgctg cgcgacggca
     3961 ttgtgcccgt gcgtgtcgaa gtgctggaat gggggagcgg aaaataccgg tgacggccac
     4021 cccgtacctt ccaaagcgcg cgttgcagct tcacccccgg cctggccctc tgaaaacctt
     4081 cattcccctg accgcgttga tctaaataag gccggaccgc tgcccccaca ggtgggagcg
     4141 agcggtccgt tgccgtgatt acgttgccgt gattacagtg accagccctc gcgatagggc
     4201 gtgcgcacgt actggttcgc atcgtcgaga ttcgttaccg tcatgcggtc gttgtcccat
     4261 tcgatgggcg tatcgtcgaa gcgcttggcg atattcccca gcgcgcaaat ctcggaaagc
     4321 ggaccggagt aggaaaagtc ggcacacgcc tttgttccat tccggatcgc gcggacccag
     4381 tcgccctcaa tgctttcgcc ttcaatgcgc gggatggacg gttccggcgc cttgaatttc
     4441 ttcatgcggg cctccggcag caggcggggc cgttcgccgt aggtgtcgca ggtcatcatg
     4501 ccgtcgctgc cgacaaacag gatgccgccg gttgggtcgc cgatctgttc gccgaccggc
     4561 actcccgccg gccgcggaat atgaagtccc gtataccacg tgaccgacac gggcggcatc
     4621 tttccacgtt tggggaactc gaaggtcacg atcgcggaca cgggatgcat ctcgtccgtg
     4681 gtgccggtcg acgtcgcgct gatccgggag ggatggccga gattcaacgc ccatttcacc
     4741 ggatcgaggc tgtgcgcacc acgatctgcc atccatcccg aaccgaacgc ctgaaaggcg
     4801 cgccaggcca tgggatgata acaagaatgg tacggccgtg ccgccgcagg ccccagccag
     4861 agatcccagt tcagcgtttc gggcacggac atcgcctcct cgggcacgcg cccatccttc
     4921 ggactccagc ccgcgtgccc ccagggatag tacgaatcgg agcagaaggc atggacttca
     4981 cgcacctcgc caataacgcc ggcctggatg gattccacaa ccgtgcgcgt gccgttgccc
     5041 gcatgcccct ggatgcccat ctgggtgacg acgccggcct ctgcggccgc ctgtgccagc
     5101 atgcggcact cgtacacgtc gtgggcgagg ggtttctggc aaaacacatg ctttcccgcc
     5161 ttgatggccg ccatggcgat caccgcatgc gtgtgatccg gtgtcgcgat gaccacgccg
     5221 tccaggccgc tttccgccgc cagcatctcg cggtaatcca cgtaccgccg cgcgtcggga
     5281 aatgcctcaa agacaggttt tgcataagcg gaatcgacat cgcacagcgc gacgaactgt
     5341 tcgccggaca ggttccggat gttgaccgcg cccatgccgc cgaagccgat agcgccgagg
     5401 ttcagccgct cgttcgccgg ggttttggcc gcggctgcgg atgtaacgat atgtggcgca
     5461 gtggcgcacg atgcagccga caacgcggca gcgctggcca gaaacgttct ccgggacatg
     5521 ttctcacgtg cagtgcattg cttcatcagg ctacctcctt cgtgacgcga gcgcgccggc
     5581 aggcgcaaat accagggcag cgcgccccct ccctccaaca cgatgatctc caggcgctgg
     5641 aacagcatga tccacgcccg ggcagatttc aagcgttctt acagccgccc ggccgcgggg
     5701 ctagtcatca agcttcacgt gcacatattt ctggcgcggg ccaggggtca tgccttccgc
     5761 gaacgcgacc cacatgtcgt tgccgctgtt gtggtagatg atgctgacca gggtgccgtc
     5821 cacgcacgcg attctggcga tctcgatggc tttttcggcg tcgatctcgc cgtagcgatc
     5881 caggatggca ttatacagca cgtcattgtc gcgcccgttg tggttcttcc agtagagggc
     5941 gtcctctatc ggtctgtgga acggcttgag gtcggcgcag gggtgtggat tgacggactg
     6001 gttgtcggcg tattcatcga atcgcgtgtg gctggtcaac agcaggcggc cttttgcacc
     6061 cgcggcattg ggatccgcga gtgcgtaatg atattggtta gtccgtaccg ccgatttcat
     6121 gcgatcaagg gccgattgca ggtcgcggtc gaaatagagc acgtcgcgca gaagaaacgg
     6181 gaaggggata ccgtccagcg tttcgctgtc gcagaaatgc cccatgattt cactgacccc
     6241 gatccctgtg gtgttcatgc cgcccacgga cacgcccaga acgcccgcaa acccgacgac
     6301 tgcgtgtttt accccatcgt cgggagtgta gatcgccacg acgggatagt cctggagccc
     6361 ggtgtccatg gaccagtcga gattgcgcat ctggtaggtc tcgccatcga gcgtagccgc
     6421 gccccaggcg gcgaacaggc tgcaattgta ctctgacaag tcgggaatgg aggccatttg
     6481 ctggagcacg ctgaacgtga gttcaggatg tccggcatcg gcgcaaccat cggcaacgcc
     6541 ctgcacttcg gtgctccagg gcgccacatc gtaatttttt tcctgccaca gagatgcggt
     6601 tgccatgttg accatctctg cggggattcc gctcttttcg atcagggctt gtgccagttt
     6661 cgaggtcgcg gcgatctggt ccgcgaggag ctttccgtac cagtagccca gttcgtaccg
     6721 gttcccctgg attcggagca gggtcagggc gtcttcgccc tcgccaatga tctcggctgt
     6781 gcccgaaccg ccgatggtcg ccggacgggc gcaaccgcgc ggctcagctg aaagcgccgg
     6841 gccagcggcg gcttcgcccg tatccccggc cgcagcgagt gcgccgacct ggggcgcggc
     6901 tttctcttcc tggcgcattg aagccaacac cggaaatccc agcgccagaa ccaccagcag
     6961 gcctcccgcg cagctcagaa gggtgtagag gcccttgcgc agcgaccgcg ccggccgatg
     7021 ggctcccgcc gccgggtccg gcagcggcgg aaggtccagc aaaccagaca ggtctggcag
     7081 gccgcttccg ccattggaag agtccgcgcc agcgcccagc aggctggcca gatccgtcgg
     7141 gccatcaagc gcagacacgt tgtcctgctg aatgcgggct ttcagctccc tcgccttatc
     7201 gtcatcaaac cattggatca actgatcgca gacggcgatg gcctccgcgt ggcgctgcaa
     7261 ctccgccagg cactgagccc ggccgtacat aacttgtttc tgtgtcggga acgccgtatt
     7321 cagctcgtca agcagcatca acgcttccac atagtgtttt tgcgaaaaca gctgtaccga
     7381 ctgttgaaac ttctgcgatg ctgtagcctt atccatgcac atgaccctca ctcgatagat
     7441 ctcgtcaagg aaggcgcccg cgactttcgg ggcacacgat gcctcacttt agtgttttcc
     7501 tgccggagaa ttcaagtttt caagccgcgc cgtctgaaat gtggcctgga atggcatcgc
     7561 cgcgccattt gccatcttac cgcagtctgg agggcaaaac gatgcccgga cagccattct
     7621 tggacggtct gggtacgtct ggaggaagaa tggcgggggt ccaccccgtc cacgtggccc
     7681 acgctctcac gccctgaggc cgccggcagc cgttcagggt tcacccccgg ccacacgcgc
     7741 tgtgcgaggc gaattctgga atctccggtg ccgtttcttg tatgctcacg gccggtgttg
     7801 agggtagttc acggcgcgcc gtgactgtat acacgcatcg ttctgatgcc gttttccgcg
     7861 cgggttttct gctgttgcct gcggtaatgc catgcagggc acacccggga cgagcgggaa
     7921 cggccggaaa cggcgccagg gcacgagagg tatcatggct ggcacgaaag agatcattct
     7981 gtcggggatt cgtccgacgg gccgcttgca ttacggcaat tatttcggtg cgatccggaa
     8041 tttcatcgat ctgcagtcgc aggacaccat ctgctactat ttcatcgcgg attaccatgc
     8101 gctgacgacc attacggagc gcgaggattt ctatcaacaa accatcgaga tgctgcggac
     8161 ctacgtggcc tgtgggctgg atccggcgaa gtcggtcatt taccgccaga gcgatctgcc
     8221 ctgcacggcg gaactggcgc tgctgctggg gatgatcacc aacatcggat ttctcgcccg
     8281 cggcaccacg tacaaggaca agatgggcaa actcgaggag gggccggggg gcgtcgacgg
     8341 caacccgctg tcgtatggtt tgctcggcta tccggtgctg atggcggcgg acatcctgat
     8401 cgttcgggcc aacaaggtac cggtgggcga tgaccagcgc cagcatctcg aaatggccat
     8461 cgacatcgcg cagcggttca acagccggtt tggggaaacg ctcacgctgc ccgaagggat
     8521 cgcgcgcaac gccctgcgcc tgccgggcct ggacggcgga gcgaagatgg gcaaaagcga
     8581 gcacaacacg ctcgacctgc tcgacgatgc ggccacggtg ctgaaaaaga tcaaagcggt
     8641 gcctaccacg accgatcccg aacccctggg cacggacagc gatcatccgg atgacgtcat
     8701 tccacggatg cccaacagcg tgggcgccct gtaccgcctg ttgaatctgc tggctccgga
     8761 agcggtgtac ctggattttg tgggaaaata ccgccgtcgt gagaaattct acggcacttt
     8821 aaaaaccacg ctggcggagt atgtatcggc attcaacgcg cccatcatcg aacaatacaa
     8881 cgcgcccggc aactcacgtg aggcggtcga ggaattcctg cgcgagaatg ccaggaaggt
     8941 cacgcccgtg gcactggaca ccgtcgagac ggttcgtgcg gccatgggca tcggccggag
     9001 cctgtaccgg gcgtaacgcg gtcagccgcg cgggagggtt gccatgcaca tcctgattgt
     9061 cggggggact cgctttctgg gtgcggcggt ggcccgtgaa ctcgtgaccc gcgggcaccg
     9121 ggtttcgctg cttcaccgcg ggacaacctc tggcaatgtg ccgcccgaag ccgagcacct
     9181 cctgggcgac gcgcgcgacc gcgcctttgt ggaatcggtg ctccagcccg gccgcttcga
     9241 cgcggtcgtg gacacgatcc tgacctccga cgacctccag tggtacctcc cgctgctcca
     9301 caagaccgtg ggccagctcg tgcattgcgg gtcgacgggg gtgtacgcgc cgatgacgac
     9361 ggtgcccgcg cgcgaatacg atccgactcc atgcgcaccg gaactgggcg gattcgcaga
     9421 aaaactcgcg caggaccgag tgctgctgga ataccacgaa cacgcgggct ttcaggtgtg
     9481 ctcgttgcgt gtgagcaacg tgtttggcgc gggcgacgtg ccgctcgaca tctggggggc
     9541 gcggaatccg cagtattttc agcgtttggc cgatggcgaa gagatctggc ttcctggaga
     9601 cggcacgacc ctgctccagc ccgtgcatgt ggacgacctg gcgcgggggt tccgggccgc
     9661 cgtggaagct ccgtcccgca caaccggaca gatctttaac ctgtcgtcgg atcgggcggt
     9721 tacattgacc cgatacgccg aactggccgc ggaactgctg ggctctgcat ccatgtttcg
     9781 ctgcgtgccc atggaggaga tcctggcgac aggccgcgcg aacgaggccg gactccgctt
     9841 catttgtgag catatgtgca tcgacagctc aaaagcgggc cgcgtgctcc attatgagcc
     9901 gaaagtctct gtgcgggagg gccttcgaga cagcctcacc tggatgatcg acacggggcg
     9961 gctgcgggct gaacggcctg cataatccgg cctttccatt cataaccaat cgtgataaac
    10021 tctactttgc acatcttgtg caaaaaaggc tgcaagatca catgaaaaac cgaaccctgc
    10081 aggtcgtata tatcctctgc tcgacgtacg acgacgaggg gtatcccctg cggttttacc
    10141 gcgggattct gccgtcgaat acgctgaact gccttcgttc gctgacgctg gacgtggcga
    10201 cgagcggcgc gctgggcgac aatgtgtccg tccaggtatc catttacgac gacaccgtcg
    10261 aaccgatccc ggtgcggcgc atcgcccgga aactccgccg gcgcaacacc gagcttctcg
    10321 tcgggttggt cggggtgcag tcgaaccagt tcatccgggc cacagatctc gccatgcagt
    10381 tccgcgatct gggggtgcag gtcgtagtgg gcggatttca cgtcagcggc atgctggcca
    10441 tgttcgatga cgtcacgccg gacctgcagc gcctgctcga cctgggcgta tcgctgtttc
    10501 aaggcgaagc ggaggccccg gacgcgctgg ccgggctctt gcgcgacgcg ctggcccgcg
    10561 aactgcagcc catctaccgt cagacggcgt atccggtact gaccgattcg ggcgcgcccg
    10621 aaccggacct cggctatctc aaacatttca aatgcagctc gatgggcacg ctcgacacga
    10681 gccggggctg cccgttcggc tgttcgttct gcaccgtcat caacgtccag gggcgccgcc
    10741 tgcggagccg gtcggccgag tgcattctcc aatcgatccg caggagttac gagcgcggca
    10801 tcaacatgtg gttcttcacc gacgacaacc tggcgcggag cccggtctgg gaggagcttt
    10861 ttgacgggct ggccgcgatg cgtgacgaag ggatggacat caccttcatg atgcaggtgg
    10921 acacgcgcgc atacgccatt cccggttttg tcgaaaaagc gcagcgcgcg gggtgctaca
    10981 tggtgttcgt cgggatggaa agcgtcaatc ccaaaaacat cgaagcgatg gacaagcgcc
    11041 agaacgatgt tgaagattac gcgcacatgg tcgaggtctg gcaccaggcg gaagccctcg
    11101 tgcacgtggg atacatcatc ggcctgccgg acgacacgcg tgagtcggtc cggcgcgaca
    11161 tcgaaacgct catgaacgag gtgaaagtgg acgaggcctc gtttttcatg ctgacgccgc
    11221 tgccgggttc ggctgaccac aagcgcatgg tggaggagtg cgtcccgatc gacgcggatc
    11281 tcaacaatta tgacagcctc cacgagacct tccggcatcc gcgcatgtcg ccgggtgaat
    11341 ggaccctggc gtataag
//
LOCUS       DAOPBQ010000043        11351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_5620_length_11351_cov_3.746370, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000043 DAOPBQ010000000
VERSION     DAOPBQ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11351
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5620_length_11351_cov_3.746370"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /gene="dnaX"
                     /locus_tag="PKW60_02605"
     CDS             <1..1512
                     /gene="dnaX"
                     /locus_tag="PKW60_02605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPJ98354.1"
                     /translation="CGTCENCTSIAAGRNIDVLEIDGASNNSVDDVRQIRDNVRLVPS
                     SSRYKIYVIDEVHQLSSAAFNALLKTLEEPPAHAVFILATTEAHKVPATIISRCQRYD
                     FRRVGQDDICALLRRILDQEQVACSDEVLLAIARAADGGIRDAQSILEQLVSYCGQEI
                     TFEDVFEVLGLVDWEQLNALCDALLDSDIAVQLQIVDDIVATGKDLSQFVQDIIRYFR
                     NLLVCKTAKAQARELVALPEHELAAMQERANRFTLLRLIQLIEQFAELSKGFDSQLAQ
                     RLALETLLIRVSKVAAEVSVDTVLEKLVQLGAGGLGPAAAASPPPPASARASSRKGSS
                     SPPKRAPVPSPVPAQVSEHESPVAATRLTVTDANLRESWHRVAERAGSENLNLGIWLG
                     QASPAGLEDGIVSLEFTRDQQNTRDYLERPENRAEIERILQAVTSNLTGYRTRAAEQA
                     PETPRPAQQTQPPPASTHVNPELLRQALKDPHIAKVVDIFKGEIIGIKHHIKE"
     gene            complement(1705..3534)
                     /locus_tag="PKW60_02610"
     CDS             complement(1705..3534)
                     /locus_tag="PKW60_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPJ98355.1"
                     /translation="MNPRAAIALLAVFTVLCGAYWLMLRHEEGARQTEFQAGLLFDFQ
                     ADALRQVSIQVEDRNPTVGVTDGDGHWRIVAPYELPAQNEVWTKVATNLAELHRDRLI
                     EDAPVDLATYGLDDPRLQIEFTTADGGSHRVVFGKIGPMQVNRYAQVDGGQVILAPHA
                     QYEQLNRQLLDLRQRYIFDTGPEGITRIEFARIRQPEENEAKPEGPYPIEEMGVVVVE
                     QVSEGQWRMLEPFEASANTELVLGLARELQFAIGSEYVDKPASLDEYYLNPPGARITI
                     FSGPDSTPQTLLIGGGTRTNDRPMLFAKRTDAPTIFQIDGALYGYFPKTPDAYRENRL
                     LTRSALDLERIDYQTGEASVALVKGEDKRWRLAEPLQEPADQEMMSGFISTLLKVRGE
                     SFPPISLAEAGLAEPAITLKLTFKDDNAPVLIRIGKATEDGQQYYATLDTGVITLVAS
                     SHVRAITRELFDFREKRIFKVDANAVSRVKLRFEDEDYTFVKGRRWTIEQPEGKVWDS
                     QDDAKALLEAVATIDASGMASLAAPDDLAPYGLDAPLMELTITTSSESGAAADTGPLR
                     IGSPLHDPSYLRFATVKGRPEVFHVNQSVIDKVREALKGVRDR"
     gene            complement(3531..5177)
                     /locus_tag="PKW60_02615"
     CDS             complement(3531..5177)
                     /locus_tag="PKW60_02615"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HPJ98356.1"
                     /translation="MKNVRTVFGILALAGLALGLNVLVFKRDPFAFSVMLPLCFGFLA
                     ACGWIAIGVSRMVESIERGKTVSTVSAVVASLAFLGICVVLYAFVRHAGFSWDLTREG
                     RTPLSEQTVQLLRGLDREVAVYGIFLKAGDRDVQIRSEKARRFLERCQHYTSNLNVEF
                     FDPEQNVLKVESLELGNLSPQGAVVLKCGTRRKIINFSNVNDRLEEGAFTQGLIYVAR
                     DSVPKLYFLTGHDEPPLPALSELTARLAEESYQVEEFAINVLDPQIPSDCNVLVINGP
                     KGDLQLPEVKAIHEYLQRGGRMLVLLEPWRVISTGVEHFLPMLRQNYGINVGTDIILS
                     RAKSPQEMVLVVFTPNLGLIGVDAPDIPRRGSYNEEHLITRGFDQNMVFSLSRSVNLT
                     PEMPEGVAGDVLLYSLPGTWAETNLNQLFDERKANPDEGDLKGPVGVAVAVTANTAVQ
                     VADSDRLRDTRIVAVGDQDFVSNDKIRIVGHWNFLLNSLAWLTETEELVGVRPARLED
                     PPLRLTPAQDRAIAWITILGAFQTIAVIGVVIFVLRRKYQ"
     gene            complement(5182..6015)
                     /locus_tag="PKW60_02620"
     CDS             complement(5182..6015)
                     /locus_tag="PKW60_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPJ98357.1"
                     /translation="MRNTWAVCKREFICFFITPVGYVVTGVFALVSGIGFAASFIGYA
                     RATQSPSAYGYTGVPDFEETLLSPFLVFCGLLIMFIGPLITMRLLAEEKNRGTAELLL
                     THPLRNREIVFGKYGAALGMLLVMMAVVSIQMAVVWYFVPHVEPAVLGFGVVTVFLMG
                     AAFLSMGLFVSAITRNQVTAGTATFGLWFVSYVLGSLSRDLPDAIDLPENWFPGAVRA
                     AGFGYSLFRSLATELPLDVHAREMAQGVLQPEDIVYYLLFSAFWLFLTLRVLDTRLWR
                     A"
     gene            complement(6012..6947)
                     /locus_tag="PKW60_02625"
     CDS             complement(6012..6947)
                     /locus_tag="PKW60_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98358.1"
                     /translation="MIVLTGVTKYYGPIAAVENVTFSVEAGEVIGFLGPNGAGKTTTL
                     RILTGFTAASRGTAKIAGFDVNDHPIEVKRRVGYLPESVPLYREMIVRKFLGYVAEIK
                     GLQGKKRQHEVGRVMERCGLSHMAGRTIGNLSKGYRQRVGLAQALLGNPPVLILDEPT
                     VGLDPRQIVEIRDMIKSLAREHTVLLSTHILPEVAMVCERVVIINRGHVVAEDSMANL
                     AGASGDKRVFDADILGDETAVRITLAGVRGVREVVVESSGCYRITTDPDERMGVLLTT
                     ALVRANHGVDNLRERTRTLEDVFIEAIGADEGDAA"
     gene            7312..7503
                     /locus_tag="PKW60_02630"
     CDS             7312..7503
                     /locus_tag="PKW60_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98359.1"
                     /translation="MAMQVQCCVCKRIRLEDRWHSADDALSPLVRISYGYCPECAVEA
                     VAKLRQEAQGTAFSQASVA"
     gene            7824..8453
                     /locus_tag="PKW60_02635"
     CDS             7824..8453
                     /locus_tag="PKW60_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98360.1"
                     /translation="MRIMRDWVPIYVLLGLVGSMMTLLVIGEELTTKRKKRFFAFNVG
                     DMIDAVYHRQHRNVYSFFEAISFRTVVVTLTVFGLSGLALTFLITFSRVKSLALLAGA
                     IAFGFELWLARRREAQEAQKRPWTAQDAVGRLAHITLTIPGAGIGQGAVALRLAAGEE
                     AEPVTFHAVTQGQGLTQGSYAHVLRAISKDTVEVQPAPMSAAPPPLPSQ"
     gene            complement(8769..10208)
                     /locus_tag="PKW60_02640"
     CDS             complement(8769..10208)
                     /locus_tag="PKW60_02640"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPJ98361.1"
                     /translation="MELRQINEYLWEIPKRGKMRVPGRIYADRQLIRKIQADNAAAQV
                     ANVACLPGIVGYSLAMPDAHWGYGFPIGGVAATDPDNDGVISPGGVGYDINCGVRLMT
                     TGLDYGELRARMHDIVNGLYRDVPCGVGSSGAIPRLSPKDMRQVLTDGAAWAVEQGYG
                     RPEDLAHTEERGRLNGADPGVVSDRAKSRGEDQLGTLGSGNHFLELGVIDRIVDEDAA
                     AAFGFREEQVTLMIHSGSRGLGYQVCDDFLQVMMRAAARYGIDLPDRQLCCAPVKSQP
                     GRDYLAAMAAAANYAWANRQIMMCLARESLQNTLGASAASLDMRLVYDVCHNIAKFEM
                     HDVDGERRRLCVHRKGATRAFPGDREEVPAAYRLVGQPVLVPGDMGTGSWICAGTSKA
                     MRETFGSACHGAGRVMSRTSARKGENAATLLRELEEQGVIVMARSKKTVTEESPSAYK
                     DIDAVTAVLDTAGIARRVARIRPVGVVKG"
     gene            complement(10239..10649)
                     /locus_tag="PKW60_02645"
     CDS             complement(10239..10649)
                     /locus_tag="PKW60_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HPJ98362.1"
                     /translation="MNYRFLDHTADARVECTGATFAELLEAAARALAAVAFRHVANHG
                     EEVRRVVSIKANGPEDAIVRWLQELIYLMDVERLAPVRFEFRQASAQSIEAVVDGCLY
                     QPEDRQDEVKAATYHGMDVRQTETGWRAEVIFDL"
     gene            complement(10690..>11351)
                     /locus_tag="PKW60_02650"
     CDS             complement(10690..>11351)
                     /locus_tag="PKW60_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98363.1"
                     /translation="LKRRERKARRPSFPRESLPRFLWRCARETAWTLRTYVQIFFEFQ
                     EIWMLTRKPDDPKWATLAELRGTWARLQDRLSESNLKGRYDVAAQEIRAALTSAAERL
                     QQLSDSSRAQSRRVSKRLAKQAEDIRQYLRGFEVQLPTWRTIADAERFVADSLVKRYE
                     ELAIRHVARRRKFNAYRRDVIARFRSWRFLTLNVLLLPRMFVVELLFGVRFMFSFLTH
                     A"
BASE COUNT         2079 a         3633 c         3460 g         2179 t
ORIGIN      
        1 tgcgggacgt gcgagaactg cacctccatc gccgcgggcc gcaatatcga cgtgctcgaa
       61 atcgacggcg cctccaacaa cagcgtggat gacgtccggc agatccgcga caatgtccgg
      121 ctggtgcctt cgtcgtcgcg gtataagatc tatgtgatcg acgaagtgca tcagttgagc
      181 agcgcggcct tcaatgcgct gctcaagacc ctcgaagagc cgcccgccca tgcggtgttt
      241 atcctggcga cgacggaagc gcataaggtg ccggccacga tcatctcgcg ctgccagcgc
      301 tacgatttcc gccgtgtggg ccaggacgac atctgcgcgc tgctccggcg catccttgac
      361 caggagcagg tggcctgctc tgacgaagtg ctgctggcca tcgcacgcgc cgcggacggc
      421 ggaatccgcg atgcccagag cattctcgag cagctggtct cgtattgcgg ccaggagatc
      481 acatttgagg atgtgttcga ggtgttgggc ctcgtggact gggaacagct caacgcgctc
      541 tgtgatgcgc tgctggacag cgatattgcc gtgcagctcc agatcgtcga cgatatcgtg
      601 gctacaggca aggatctgtc ccaatttgtt caagatatca tccggtattt ccggaacctg
      661 ctggtatgca agactgcgaa ggcccaggcc cgggaactgg tggcgctgcc cgagcacgag
      721 ctcgccgcaa tgcaggagcg ggcaaaccgg ttcacgctgc tccgccttat ccagctgatt
      781 gagcagttcg ctgaactttc caaggggttc gactcgcagc ttgcacagcg gcttgccctc
      841 gaaaccctgc tgatccgcgt gtcgaaggtg gccgccgagg tctcggtgga taccgtgctg
      901 gagaaactgg tgcaactggg cgcgggcggt ctgggcccgg ccgccgccgc gagcccgccg
      961 ccgccggcat ccgcgcgggc cagctcgcgt aagggctcat ccagcccccc taagcgcgcg
     1021 ccggtgccgt ctccggttcc ggcgcaggtt tccgagcatg aatctcctgt tgcggcgacc
     1081 cgtttgacgg tcacggacgc caacctgcgt gagtcgtggc accgcgtggc cgagcgcgcc
     1141 ggctccgaaa acctgaatct cggcatctgg ctcggccagg cctccccggc cggcctcgaa
     1201 gacggcatcg tttcccttga attcacccgc gaccagcaaa atacgcgcga ttacctcgag
     1261 cgccccgaaa accgcgccga aatcgagcgt atccttcagg cggtgacgag caatctgacc
     1321 ggttaccgca cgcgggccgc tgaacaggcc ccggaaaccc ctcggcctgc acagcaaacc
     1381 cagccccccc cagcgtccac gcatgtgaat cctgagctgc tgcgtcaggc cctcaaagac
     1441 ccccatatcg ctaaagtcgt cgatatcttc aaaggcgaga tcatcggcat caagcaccac
     1501 atcaaagaat agcaccaatg cagcggggcc gggggcgcca cggggacttc cgtcagaccg
     1561 tttgggtggg aagttctgct gctgaatgct tcccaaaagc gtgagccgtg gggtggggcg
     1621 cctgaagtcc gcgcattccg cctgttcgtc acgccgccgc gaatgagccg cgtgcatggt
     1681 tgctcggcag ctgctgcggc tgggttagcg gtcgcggacg ccctttaacg cctcacgaac
     1741 cttgtcgatg accgactgat tgacgtggaa cacttccgga cggcccttca ccgtcgcaaa
     1801 acgcaggtac gatgggtcgt gcagcgggct gccgatgcgg agcggtccgg tgtcggccgc
     1861 ggctccagat tcgctggacg tcgttatggt caattccatc aagggggcat cgagcccgta
     1921 cggggccaga tcgtcgggcg cggccagaga cgccatgcct gacgcgtcga ttgtggccac
     1981 ggcttcgagc agcgccttgg cgtcatcctg gctgtcccag accttcccct cgggctgttc
     2041 gatcgtccag cggcgcccct tcacgaacgt gtaatcctcg tcctcgaagc gcagcttgac
     2101 gcgcgatacg gcgttagcat ccaccttgaa aatgcgtttc tcgcggaaat cgaacagctc
     2161 gcgggtgatc gctcgaacgt gcgacgaggc caccagcgtg atgacccccg tgtccagcgt
     2221 cgcgtaatac tgctggccgt cttccgttgc ttttccaatt cgaatcagga ccggtgcatt
     2281 gtcgtctttg aacgtcagtt tgagggtaat tgcgggctcg gccaggccgg cctcggccag
     2341 cgaaatgggc ggaaacgatt cgccgcggac tttcagcagg gtggaaatga atccggacat
     2401 catttcctgg tcggcgggtt cctgcagagg ttccgcgagc cgccagcgct tatcctcgcc
     2461 tttgacgagc gccacgctgg cctcgcccgt ctgatagtcg atgcgctcca gatcgagcgc
     2521 gctgcgggtc aggagccggt tttcgcggta ggcgtcgggc gttttaggga agtagccgta
     2581 caaggccccg tcgatctgga agatggtcgg ggcgtcggtg cgtttcgcga acagcatcgg
     2641 cctgtcgttg gtgcgcgtgc ccccgccgat caggagggtc tggggtgtgc tgtccgggcc
     2701 ggaaaatatg gtgatacggg cgccgggcgg attcaggtag tactcatcca ggctggcggg
     2761 tttgtcgacg tattcgctcc cgatcgcgaa ctggagttcc cgggccaggc ccagcacgag
     2821 ttccgtgttg gcgctggcct cgaacggctc cagcatgcgc cattggccct cgctgacctg
     2881 ctcgaccacg accacgccca tttcctcgat cggatacggg ccttcgggct tcgcctcatt
     2941 ctcctcgggt tggcggatgc gggcgaactc aatgcgcgtg ataccttcgg ggccggtgtc
     3001 aaagatgtag cgttgccgca ggtcgagcag ctgccggttg agctgttcat actgcgcgtg
     3061 gggcgccagt atcacctgtc caccgtcaac ctgcgcgtac cggttgacct gcatcgggcc
     3121 gatcttgcca aagacaaccc ggtgggagcc gccgtccgcg gtggtgaatt cgatttgcag
     3181 gcgcggatca tccagtccat acgtcgcgag gtctaccggg gcgtcctcaa tgaggcgatc
     3241 ccgatgcagt tcggcgagat tggtggccac cttggtccac acttcgttct gggccggcag
     3301 ttcatacggc gcgacaatcc gccagtgacc gtcaccgtcc gtgacgccca ccgttgggtt
     3361 gcgatcttcc acctgaatgg agacctgccg gagcgcatcc gcctggaagt caaacagcag
     3421 gcccgcctgg aactctgtct ggcgtgcgcc ttcctcgtga cgcagcatca gccagtaggc
     3481 tccgcacaat accgtgaaca cggcgagcag agctatcgcg gcgcggggat tcactgatac
     3541 ttcctccgca acacaaaaat gaccacgccg atgacggcaa tggtctggaa tgcccccagg
     3601 atggtgatcc aggcgatcgc gcggtcctgg gcgggcgtca ggcgcagcgg cgggtcttcc
     3661 agacgtgcgg ggcgcacacc caccagttct tcggtctccg tcagccaggc caggctgttg
     3721 agcaggaaat tccaatggcc cacgatgcgg atcttgtcgt tggagacgaa atcctggtcg
     3781 ccgaccgcca cgatgcgggt gtcgcgcaag cgatcgctgt cggccacctg cacagcagtg
     3841 tttgcggtaa cagccaccgc aacccccacc gggcctttga ggtcgccttc atccggattg
     3901 gccttgcgct catcaaagag ctggttgaga ttggtctccg cccaggtgcc tggcaatgaa
     3961 tacagcagga catcgccggc cacgccctcg ggcatttccg gcgtgaggtt gacgctgcgc
     4021 gacagcgaga acaccatgtt ctggtcaaag ccgcgcgtga tgaggtgctc ctcgttgtac
     4081 gatccccgcc gggggatgtc cggcgcgtcc accccaatca ggcccaggtt cggcgtgaaa
     4141 accaccagca ccatctcctg cggcgatttc gcccgcgaca ggatgatgtc ggtccctaca
     4201 ttgatgccgt aattctgccg gagcatggga agaaagtgct caacgccggt ggagatgacg
     4261 cgccagggtt ccagcagcac gagcatgcgg ccgccgcgct gcaggtactc gtgaatcgcc
     4321 ttgacttcgg gaagttgcag gtcgcctttg gggccgttga ttaccagtac gttgcagtcg
     4381 gacgggattt gaggatccag cacgttgatc gcgaattcct caacttgata cgactcttcc
     4441 gcgagccggg ccgtcaattc tgacagggcc gggagcggcg gctcgtcgtg gccggtcaga
     4501 aaatataatt ttggcactga atcccgggcc acatagatca acccttgcgt gaaagcgcct
     4561 tcttcgagcc gatcgttaac attcgagaag ttgatgatct tgcgccgcgt gccgcatttg
     4621 agcaccaccg cgccctgtgg cgagaggttc ccgagttcca ggctctccac cttgagcacg
     4681 ttctgctcgg gatcgaaaaa ttcaacgttc agattgctgg tgtagtgctg gcagcgttcg
     4741 agaaaccggc gggccttttc actgcggatc tgcacgtcgc ggtcgcctgc tttcagaaat
     4801 atgccgtata ccgccacttc cctgtccagt ccgcgcagca gctgcaccgt ctgctcggac
     4861 agcggcgtcc gtccttcgcg ggtgagatcc caggagaacc cggcgtgccg cacgaaggca
     4921 tacagcacta cgcagatccc gagaaaagcc agtgacgcca cgacggcact cacggtgctc
     4981 accgttttgc cgcgctcaat ggattcgacc atgcggctca cgccgatagc gatccagccg
     5041 cacgcggcca ggaatccaaa acacaggggc agcatgaccg agaaggcgaa ggggtcgcgt
     5101 ttgaacacca gcacgttcag gcccagggcc aaacccgcca gcgcgaggat tccaaagact
     5161 gtgcgcacgt ttttcaccgg ctcaggctct ccataaccgt gtatccagga cccgcagcgt
     5221 cagaaagagc cagaacgccg aaaacagcag gtagtagacg atgtcctccg gctgcaggac
     5281 gccctgggcc atttcccggg cgtgcacatc caacggcagc tctgtggcca gcgagcgaaa
     5341 gagactgtag ccgaaaccgg ccgcgcgcac cgcccccgga aaccagtttt cgggcaggtc
     5401 gatcgcgtca gggagatccc ggctgagcga acccagcacg tacgacacga accacaggcc
     5461 aaacgtcgcc gtgcccgccg ttacctggtt gcgggtgatc gcggagacaa agaggcccat
     5521 gctcaggaag gccgcgccca tgagaaagac cgtaaccacc ccgaacccca gcaccgccgg
     5581 ctcgacatgg ggcacgaaat accatacgac ggccatctgt atggatacca cggccatcat
     5641 gaccagcaac atgcccagtg ccgcgccgta tttgccaaac acgatctcgc ggttccgcag
     5701 cgggtgggtg agcagcagct cggcagtccc ccggttcttt tcctccgcca gcaggcgcat
     5761 ggtgatcagc gggccaatga acatgatcag caggccgcag aagaccagaa acgggctcaa
     5821 cagcgtttcc tcgaagtccg gtacgcccgt gtaaccatag gccgacggcg actgggtcgc
     5881 ccgggcgtag ccgatgaacg aggccgcaaa cccgatcccc gagaccagcg cgaacacgcc
     5941 cgtgaccaca tagcccaccg gcgtaatgaa aaagcagatg aattcgcgtt tacagaccgc
     6001 ccaggtattt ctcatgccgc atcgccctcg tcggctccga tggcctcgat gaagacgtcc
     6061 tcgagggtac gcgtgcgctc gcggagattg tcgacgccgt ggtttgcccg caccagcgcg
     6121 gtggtcagga gcacccccat ccgctcgtcg gggtcggtcg tgatgcggta acagccactg
     6181 gattcgacca ccacctcacg cacgccgcgc acgccggcca gggtgatccg gaccgccgtc
     6241 tcatcgccca ggatgtccgc gtcaaaaacg cgtttgtcgc cactcgcgcc cgccagattc
     6301 gccatgctgt cctcagcgac aacatggccc cggttgataa tcaccacgcg ttcacagacc
     6361 atcgcgactt ccggcaggat gtgcgtgctc agcagcaccg tatgttcccg ggccaggctc
     6421 ttgatcatgt ctcgaatctc gacaatctgc cgggggtcca gtccgaccgt cggttcatcg
     6481 aggatcagca cgggcgggtt cccgagaagg gcctgcgcca ggcctacccg ctggcggtag
     6541 cctttcgaca ggttgcctat ggtgcgcccg gccatgtgag agaggccgca gcgctccatg
     6601 acccgcccca cttcgtgctg gcgtttcttg ccctgcaacc ccttgatttc cgccacatag
     6661 cccaggaact tgcggacaat catctcccgg tacagcggca cgctttcagg gagatagcct
     6721 acgcgccgct tgacctcgat gggatggtcg ttgacatcaa acccggcgat tttcgccgtt
     6781 ccccgcgatg ccgccgtaaa tcccgtgaga atgcgcaggg tcgtggtctt tccggcgcca
     6841 ttcggcccca gaaagccaat cacttcccct gcttccacgg aaaaggtcac gttttcgacg
     6901 gcagcaatgg gcccgtaata tttcgtgacg ccggtaagta cgatcatgaa ttggtcaata
     6961 cctctggagg atgctcttcg cttgacattg tatactctga atgaggctct ggtttcaagc
     7021 cgcaagtgcc gtatcgctca cgccatacac cgccggaaat gcttcatttg aaaacggaaa
     7081 cgtctctcaa cgccagaatt ctgacagccc ggcatctttt tccgcgcccc ggaccctgaa
     7141 aacggaaggc agattgcggc cccagcggcg cacacatcct gtccaagccg cttgtttgta
     7201 aacagttaac cttgcacggc gtccggtacc ggttgtctgg cacgcaaaat gcttctgtta
     7261 aagtgcaaac agcccggcca tgagggccgc aatgacaagg atgtgaaagc aatggcgatg
     7321 caagttcaat gttgcgtgtg caagagaatc cgcctcgagg accggtggca ttctgcggac
     7381 gacgccctgt ctccgctggt gcgcatcagc tatggctact gccccgaatg tgcggtggaa
     7441 gccgtagcca agctccgcca ggaagctcag ggtacggcct tttcgcaggc atctgttgcg
     7501 tgaggattcg cacggccagc cttttgtatg caggtagcgg cccgctgcct gcccatgcct
     7561 gatcctgccg gtattcactg tgaccccgcc cgaaagaata tggaacttct tgcctcgtta
     7621 aagaatagat ctcagatata gagatcggct cgtttgaact gacccgataa gccgccatcg
     7681 gctgagtttt cagccgggca gtgcttttag ttcgttagag ggctgcggac gcggccctgc
     7741 cgtgctggtg gcgcggcaaa aagccgtccg caccttgcca ggttcagaca gcccaaggtt
     7801 cgcaggaata gtgttagaag gtagtgcgaa taatgcgtga ctgggttcca atctatgtgt
     7861 tgctggggct tgtcggcagc atgatgacct tgctggtgat cggtgaggag ctgacgacca
     7921 aacggaaaaa gcggtttttc gcgtttaatg tgggcgatat gattgatgcc gtgtaccacc
     7981 ggcagcatcg caatgtgtac agtttttttg aggccatttc gttccggacg gttgtggtga
     8041 ccttgactgt cttcgggttg tccggcctgg cgctcacctt tcttatcacg tttagccgtg
     8101 tcaagtcact ggccctgctg gcgggagcga tcgccttcgg cttcgagttg tggctggcgc
     8161 gccggcgtga ggcccaggaa gcacaaaagc gcccctggac tgcccaggat gcggtgggac
     8221 ggctcgcgca catcacgctc accattcctg gcgcgggcat aggccagggc gcggtggccc
     8281 ttcgccttgc tgcaggcgag gaggcggagc ccgtgacctt tcatgccgtc acccaggggc
     8341 aaggccttac ccagggcagc tatgcacacg tgctgcgggc gatctcaaaa gacaccgtgg
     8401 aggtgcagcc cgcgcccatg agcgccgctc cgcccccgct cccttcccag tgagccgctg
     8461 cagcttcgaa gctcggccgc tctacaactc tccggccaca acaagtcttg cggccgggaa
     8521 tgatgggctc cagcggtatt aacgcggact caggggaata ttctttttga gcggcagtgc
     8581 agccgccagc agcatcggct caatcgcggc tggacgcact gatttttcac gcgtgacgac
     8641 atgcaggtgt ttcattgtcc ctgctccctg gtttcgtact gcgccgcttg acgatacgaa
     8701 aacagtcctc catacgaaag gtattccgaa ctgccagggg gcgggatgcg ccgcgcctcg
     8761 tgcgtgcttt agcctttcac tacgccaacc ggccggatgc gtgcgacgcg gcgcgcgatc
     8821 ccggcggtgt ccaggacggc cgtcaccgca tcgatgtctt tgtacgcgct ggggctttcc
     8881 tcggtgacgg tctttttgct gcgcgccatc acgatgacgc cctgttcttc cagttcgcgc
     8941 aacagggtgg cggcgttctc gcctttgcgg gcggaggtgc ggctcatgac gcgcccggcg
     9001 ccgtggcagg cgcttccaaa ggtctcgcgc atggccttgc tggtgcctgc acagatccag
     9061 gagcccgtgc ccatgtcgcc cggcacgagg accggctgac ccacgaggcg gtaggccgcc
     9121 ggcacttctt cgcggtcgcc cggaaacgcg cgcgtcgctc ccttccggtg cacacacaag
     9181 cggcggcgct cgccgtcgac atcatgcatt tcgaacttgg cgatgttgtg gcagacatca
     9241 tagaccagcc gcatatccag cgaggcggcc gacgccccga gcgtattctg aagcgattcc
     9301 cgtgccagac acatcatgat ctgacggttg gcccacgcat aattggccgc ggccgccatg
     9361 gccgccagat aatcgcgtcc cggctggctt ttgacgggcg cgcagcacag ttgccggtcc
     9421 ggcagatcga tcccgtatcg cgcggcggca cgcatcatca cctggaggaa atcgtcgcag
     9481 acctggtatc ccagcccgcg gctgccgctg tggatcatga gcgtgacctg ctcttcgcga
     9541 aacccgaacg ccgcggcagc gtcctcatcc acgatgcggt cgatcacgcc cagttcgagg
     9601 aaatggttac cggaaccgag cgtgccgagc tggtcttcgc cccggctctt cgcgcggtcc
     9661 gacaccaccc cgggatcggc gccgttgagc cgcccgcgct cctcggtgtg agcgaggtct
     9721 tcgggcctgc cgtagccttg ctcgacggcc caggccgcgc cgtcggtcag tacctgccgc
     9781 atgtccttcg ggctgagtct gggaatagcg ccgctcgagc ccactccgca cgggacgtca
     9841 cggtacaggc cattcacgat gtcgtgcatc cgggcccgca gctccccgta gtccaggccc
     9901 gtggtcatca gtcgcacgcc gcaattgatg tcgtaaccga cgccgccggg cgagatcact
     9961 ccgtcgttgt cggggtcggt ggctgcgacg ccgccaatgg gaaagccata gccccagtgt
    10021 gcatccggca tcgccagtga atagcccaca atgcccggaa gacacgccac attcgccacc
    10081 tgggccgcgg cgttgtccgc ctggatcttc cggatgagct gccggtcggc gtagatccgc
    10141 ccgggcacgc gcatcttgcc ccgctttggg atttcccaga ggtactcgtt aatttgtctc
    10201 agttcgatgc ccgccacgac atgctgctca ctgcgttatc acaggtcgaa gatgacttcc
    10261 gcgcgccatc cggtttcggt ttgccgaacg tccattccgt ggtaggtggc cgctttgact
    10321 tcgtcctggc ggtcttccgg ctggtagaga cagccgtcca cgaccgcctc gatgctctgg
    10381 gcgctggcct gccggaactc gaaccgcact ggagcaagcc gctccacatc catgaggtaa
    10441 atgagttcct gcagccaccg cactatggcg tcttccggcc cgtttgcctt gatcgagacc
    10501 acacgccgga cttcttcgcc gtggtttgcc acgtgccgga acgctacggc ggccagcgcg
    10561 cgtgcggccg cctccagcag ctccgcaaac gtggcgccgg tacattcgac ccgcgcgtcc
    10621 gcggtatggt ccagaaaacg gtaattcata gtcaggtacg gcgcacaccc gtacgccgac
    10681 gcatctgtgt caagcgtggg tcaggaaact gaacatgaac cgcacgccaa acagcagttc
    10741 gaccacgaac atgcgcggca gcagcaatac gttgagcgtg agaaagcgcc atgacctgaa
    10801 ccgtgcgatt acgtcccttc ggtacgcatt gaacttccgg cgccgggcga cgtggcgtat
    10861 tgccagttcc tcgtatcgct tgacgagcga atcggccacg aaacgttcgg catcggcgat
    10921 ggttcgccag gtcggcagct gcacttcaaa acccctcagg tactgccgga tgtcctcggc
    10981 ctgtttcgcg aggcgtttgc tcacccggcg gctctgcgcc cgggaactgt cgctcaactg
    11041 ctgcagccgt tcagcggccg aagtgagcgc cgcccggatc tcctgcgccg ccacgtcgta
    11101 gcggcccttc aggttggatt cgctgaggcg gtcctgcagg cgggcccagg ttccccgcaa
    11161 ttcggccagg gtggcccatt tcgggtcgtc cggcttgcgg gtcaacatcc agatttcctg
    11221 aaattcaaag aagatctgca cataggtgcg gagcgtccag gcggtctccc tggcgcagcg
    11281 ccacaggaag cgcggcaggc tttcccgcgg aaaagacggc cggcgcgctt tgcgttcgcg
    11341 gcgtttcagc a
//
LOCUS       DAOPBQ010000044        11222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_5690_length_11222_cov_4.549387, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000044 DAOPBQ010000000
VERSION     DAOPBQ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11222
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5690_length_11222_cov_4.549387"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PKW60_02655"
     CDS             complement(<1..721)
                     /locus_tag="PKW60_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPJ98364.1"
                     /translation="MANPMPSTAVLDILRRGTVIPAHPLALTQDGRFDEKHQRALTRY
                     YCAAGAGGVAVGVHTTQFEIREPQHGLFRPVLELAKETADACAASLGRPVLLIGGICG
                     KTPQALEEAAFLKETGYDMGLLSLAVFKEESVPQMIAHCRAVAEILPIMGFYLQPAVG
                     GRVLPAEFWRQFAEIENVIAIKMAPFNRYQTFDVIRAVAEAGRADDIALYTGNDDNIL
                     VDLLTPYRVRVGGDTVALRIVG"
     gene            complement(723..1799)
                     /locus_tag="PKW60_02660"
     CDS             complement(723..1799)
                     /locus_tag="PKW60_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPJ98365.1"
                     /translation="MPATRRTRKKPLQVKTAIPPVKTEAELDALLSAPAPELVEFMAR
                     LNGDLLILGAGGKVGHTLARMAVRAVEASGVQRKVIGVATFSSPGMRKALDQIGVETH
                     QADLMKPGALDQLPDAENVLYLVGRKFGSTGAEWDTWAVNVQLSGECARRYRKSRIVS
                     FSSGNIYPFLPVGSGGATEETPVAPVGEYAMTTLGRERMFDYVAHHEGTKVVQFRLNY
                     AAELRYGIVHDVATKVWNREPIDLSMGHVNVVWQGYVCNVALLSFGLAASPARILNVA
                     GPETVSIRWMAERLGKLLGKAPEFVGEESPNALLSNAATCYKLFGYPQVSVDALIEWV
                     AYWVAHDGASLGKPTHYETRDGKF"
     gene            2009..2950
                     /locus_tag="PKW60_02665"
     CDS             2009..2950
                     /locus_tag="PKW60_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPJ98366.1"
                     /translation="MPNEVSRRSFLQSSSLAGAGLAALTPLSAVKKTYQQGTSPWPIC
                     FNTSSIRPATLEQKIEATVAAGYDGIEPWIDELEEYEKAGGNLQDLGKRLSDLGLFVP
                     NVIGLWGSIPADPAQWEESLAPTRNRMRMMAAIGSRHAACIPPGRPADVDRTWCARCY
                     RDLVKIGREEYGISAALEFVGFMESIHRFGDAAGIAIDANIPDACLIMDTFHLYRGGS
                     GFEGIRHIRGNFIANFHINDVPSEPAREEMVDKHRIYPGDGILPLASVFQQLKEIDYR
                     GALSIEMFNEEHYAQEPKVVAETALRKTLAVIEGSGV"
     gene            complement(2984..4240)
                     /locus_tag="PKW60_02670"
     CDS             complement(2984..4240)
                     /locus_tag="PKW60_02670"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPJ98367.1"
                     /translation="MKNASKRGGDVLGIHYGLVILVMSTCVVFGALGLARFGYSILLT
                     PMQSALGLDNTQAGVLATANLAGYLVLSVVGGALASRYGPRAVIAAGLALAGAAMILT
                     GFSEGMATAALWRGVTGLGSGASNVPVMALLAAWFAPRRRGLAAGVGVSGSSFALILL
                     GPSVPPLLTHFGEAGWRVCWGLLGAITLILAAAASLLLRNHPDDVGALPLGALPGEPP
                     PAPRAAPLRWASVYRSWTVWHLGLVYVAFGFAYIIYLTFFVKHLMSDGGYTHSEAGRL
                     FMLMGWCSVLCGLIWGAVSDALGRKWALVIVYGIQTAAFTLFALAPNPLGFTVSAVLF
                     GLTAWSIPAIMAAACGDMLGPRMAPAALGFITLFFGIGQAAGPSVAGALADASGSLSS
                     SFLLAGGVAVLGGAGAALLRPSSAGS"
     gene            4399..5367
                     /locus_tag="PKW60_02675"
     CDS             4399..5367
                     /locus_tag="PKW60_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98368.1"
                     /translation="MPCFMVLMAAMLAAAPETTEPVLVVANKHDDTLSFINLASLEVM
                     ETIPTGPNPHEMAITPDQRIIYLSNYAPPGNTISVIDLVQRKHIKQIPTGDYTRIHGA
                     TMAPDGKHAYFTAGQTGFVVEVDTATHEVTRGIPTHGTISHMVLVSPDGTRLYTANIT
                     SENVSVIDRASGELLTQIPCGPGVEGMAFTPDGKQLWALNQTGGSITIIDTATHAPLE
                     TFACPGMPVRILFTGDGARAYIPGWIEEGVLIVLDVAARKELARVRVGRFAIGIVLSP
                     DGTRAFVGCEHNDGVHVVNTETLTVEKVIATGNGPDPMMLWYPPER"
     gene            5433..6602
                     /locus_tag="PKW60_02680"
     CDS             5433..6602
                     /locus_tag="PKW60_02680"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPJ98369.1"
                     /translation="MILAMISICFAANDVTPLPVQWDLARLAQVPATYPADGFWAEGV
                     QALFYEGLPLEGRPTRVFAWLGVPERSDGEPVPGMVLAHGGGGTAYDEWVRLWNARGY
                     AAIAMDMDGTVPRGDYKSRPTHEHSGPTDFGTASPMMGAVEDYWIFHAIADIALAHSL
                     LASQPGVDPARIGLTGISWGGFLTNIAAAVDSRFQFAIPVYGCGFINELTWKPAFESM
                     PPGKAEEWMRLWDPSGYVGAITMPTLWVNAPNDPHYHLGAYSRTYLRAGGSKTLSVQP
                     GLGHSHHHGWAPKEIYVYADSQVRGGAPLLRIVAEAEEGTTALALFEGGTPVSAELIW
                     TRDASNWSQCTWETAPAILQDGRVSAEIPSGCAAYFINLTDDRGLTASTVCRTRN"
     gene            complement(6623..7315)
                     /locus_tag="PKW60_02685"
     CDS             complement(6623..7315)
                     /locus_tag="PKW60_02685"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HPJ98370.1"
                     /translation="MKHTLPLFVAVVTLGVMGTLALAQPLDGPPRPPRAAGPLMEQLA
                     GMREADIRSLVETVRMVRLSRELGLTDEQTVVLVRQYNEARENGVKVQRERHELMREL
                     RGLVSANAPESDIDQKLEQLIAKDREALATRLDAFEKASVPLTPAQQARLYLFMGDFD
                     KRMRTLIETARKQLREQRGESARDGFPRQGGLRRGAGDGRAGRGEHGRQRGPRPMPQT
                     PPRPVEPPSEQP"
     gene            complement(7319..7930)
                     /locus_tag="PKW60_02690"
     CDS             complement(7319..7930)
                     /locus_tag="PKW60_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98371.1"
                     /translation="MNNDQLSEVLSAYLDGESRDPEAVERLLESDPAARRRYDILLSQ
                     SQALRRLPSPEAGPEFAVRLLARLREQQTRPYRGWVMGVAPWMLAAAVAFVLAAAYLA
                     IQAPPESRVSPIATEIQKWREIDPDLLDSILLERIAADPDALDALESEYAGVSPDADG
                     RYAADGAPAGMYEDLPLDTWIEDLNPDEEAVFRELLVAYAMEG"
     gene            complement(7927..8526)
                     /locus_tag="PKW60_02695"
     CDS             complement(7927..8526)
                     /locus_tag="PKW60_02695"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPJ98372.1"
                     /translation="MVEVVRRMNTPSDWELVALARAGDDQAFGALVRRHENGLIAFCH
                     RMTGSRDDAEDLAQECFIRVYRYLWRLRPEAKFSTVLFGMARNLTLNFLRDAKRRGRC
                     RTQPLTSHDAVQERVCNPLHTPDSQARLSELEARLERTLELLSPEHREILVLRELNGL
                     DYDAIAAVIRRRKGTVRSRLARAREQFRIRWLELGGGDL"
     gene            complement(8606..9001)
                     /locus_tag="PKW60_02700"
     CDS             complement(8606..9001)
                     /locus_tag="PKW60_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPJ98373.1"
                     /translation="MRTSRLQGMQALHDIEQRSFQLTLSIIELVRQIEPELPQALADR
                     IIITATNFGAGVSEALVHSTPRNQINKLAGARRATREMAFLLRLLNGIQMAAEPALRP
                     MLEEAAALQDELTRLILRVRQEAERASSK"
     gene            complement(9055..10452)
                     /gene="cysS"
                     /locus_tag="PKW60_02705"
     CDS             complement(9055..10452)
                     /gene="cysS"
                     /locus_tag="PKW60_02705"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPJ98374.1"
                     /translation="MSIVFYNTLTKRKDTFVPLEAGKAGIYTCGPTVYNYAHIGNLRT
                     FLFEDLLRRHLEYRGYAVTHVMNLTDVEDKIIRTCRETGESLESLTGRYAQAFLEDLD
                     TLRILRPHRMPRATETIPEIVDFIKILRDRGHTYEVDGSIYFRLASFSGYGKLSGFDI
                     DTLKANAGGRIDSDEYETEDARDFALWKAWDEDDGDVYWDTELGKGRPGWHIECSCMS
                     RKHLGDSFDIHCGGVDLVFPHHENEIAQSEAATGKPFVSYWLHAGHLQVEGRKMAKSY
                     GNTYTLRDLLDKGYDPVAIRWALSATHYREPSNFTFNGLEAAAQSVRRIQDFRLRLAE
                     VEGAGADPADEAAACEKAFGEALDDDLNISAALAAVFDFVRDTNKRIDDGSLGAEGAA
                     NALALLDRLDAVCALFPAKEADAVPQDVLNLVNDRQRARREKDFARADEIRDTLKAMG
                     WVVEDTPDGPRVKRA"
     gene            10788..>11222
                     /locus_tag="PKW60_02710"
     CDS             10788..>11222
                     /locus_tag="PKW60_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98375.1"
                     /translation="MRRAHQLVLRIILGGIMALGAANPALGADNGGGAAAGTPPQEMN
                     GLKRLHFEDFEKDPEIIAKQWQPTDPKAWAVVEDGTGHAYALKDASDYTPPVRSPLSI
                     SLLKGFEVTDFILQADMKQTGKEYGHRDMCVFFGYQDPAHFYY"
BASE COUNT         2096 a         3635 c         3472 g         2019 t
ORIGIN      
        1 cgcccacgat gcgcagggcc actgtatcgc cacccacgcg cacgcggtag ggcgtgagca
       61 ggtccacgag gatattgtca tcgttgccgg tatacagagc gatgtcgtcc gcccggcccg
      121 cctcggccac ggcccgaatg acgtcgaatg tctgatagcg gttgaatggc gccatcttta
      181 tggcgattac gttttcgatt tcggcgaatt ggcgccagaa ttcggcggga agcacccggc
      241 ctccaacggc cggctgcagg taaaatccca tgatcggcag tatttcagcc acagcccggc
      301 aatgcgcgat catctgcgga acgctttctt ccttaaatac cgccaggctg agcaggccca
      361 tgtcgtaccc ggtctctttc agaaacgcgg cttcttcgag ggcctgtggc gttttgccgc
      421 aaattccgcc gatcagcagc actgggcgcc cgagggaggc cgcgcacgcg tcagcggttt
      481 ccttcgccag ctcgagcacg gggcggaaca ggccgtgttg cggctcgcgg atctcgaact
      541 gcgtggtatg gacacccacc gccacaccac ccgcgcccgc ggcacagtaa tagcgcgtca
      601 gggcgcgctg gtgcttctcg tcaaagcgcc cgtcttgcgt caaggcgaga ggatgagcgg
      661 gaatcaccgt gccccggcgc aggatgtcga gaacggcagt ggagggcatt ggattggcca
      721 tactaaaact tcccgtcgcg ggtttcgtag tgggttggtt tgccgaggct tgctccatca
      781 tgggccaccc agtacgccac ccactcaatc agggcatcga cgctcacctg cggatacccg
      841 aacagtttgt aacacgtcgc cgcattgctc agcagcgcgt ttggcgattc ctcgcccaca
      901 aattccggcg ccttgcccag gagtttgccc agccgttccg ccatccagcg gatcgacacc
      961 gtttccggcc cggccacgtt caggatgcgc gcgggcgacg ccgccaggcc gaaactgagc
     1021 agcgcgacgt tgcagacgta accctgccag acaacattca catgccccat ggagaggtcg
     1081 atcggctcgc ggttccagac cttcgtggcc acatcgtgga cgatcccgta gcgcagttcc
     1141 gcggcataat tgagccggaa ctgaacgact ttcgtgcctt cgtggtgcgc gacgtagtcg
     1201 aacatgcgct cgcgcccgag cgtggtcatt gcgtattcgc ccacgggcgc cacgggcgtt
     1261 tcctcggtcg ctccgccgct gcctaccgga agaaagggat agatgttgcc ggacgaaaac
     1321 gacacgatgc gcgacttgcg gtaacggcgc gcacactcgc ccgacaattg cacattgacc
     1381 gcccaggtgt cccattcggc gcccgtcgaa ccgaatttac ggccgaccag gtacagcacg
     1441 ttttcggcat ccggcagctg gtccagcgcg cccggcttca tgagatcggc ctggtgggtc
     1501 tcgaccccga tctgatccag tgcttttcgc atgccgggcg aagaaaaggt ggccacgccg
     1561 atgactttgc gctgcactcc cgaggcctcg accgcgcgca cggccatccg cgcgagagta
     1621 tggcccactt tgccgcccgc ccccaggata agcaggtcgc cgttgagcct ggccatgaac
     1681 tccaccagct ccggcgcggg cgccgacaac agcgcgtcga gttctgcttc ggtttttacc
     1741 ggcgggatgg cggttttgac ttggagcggt ttcttccgtg ttcgtctcgt ggcgggcatg
     1801 atatttcctt ctggttgcgc ggaaatcagt gtgattgcca ttgtgccaca gggcgtgcgg
     1861 ggctgcaatg tcaggcatgc ccgccgcgat tggtgttcaa gcgaaaaggc gccccccgcg
     1921 cgcccggcag tccgcttcaa tctagatttt tccgctggat tctgcataat ggatgcaaat
     1981 ccgcagtggc gcaaaccgga gagagtacat gccgaatgaa gtcagtcgaa gaagttttct
     2041 gcagagcagt tcactggccg gcgcagggct ggccgcgctg acgccgctga gcgcagtgaa
     2101 gaagacctac cagcaaggga caagcccctg gcccatctgc ttcaacacga gttcgatccg
     2161 cccggcaacg ctggagcaga agatcgaggc gaccgtcgcg gcgggctacg acggcattga
     2221 gccctggatc gacgagctgg aggaatacga aaaagccggc ggcaatttgc aggatctcgg
     2281 caagcgcctg agcgatttgg gcctcttcgt gccgaacgtc atcggactgt ggggatccat
     2341 cccggccgat cccgcccagt gggaggaatc cctcgcaccc acacgcaacc gcatgcggat
     2401 gatggccgcc attggttccc ggcatgccgc ctgcatcccc ccgggacggc ctgccgacgt
     2461 ggatcgcacc tggtgcgccc gctgctaccg ggatctggtc aagatcggcc gcgaggagta
     2521 cgggatctcg gcggcgcttg aattcgtggg gtttatggag agcatccacc ggttcggcga
     2581 cgcggccggc atcgccatcg acgcaaacat ccccgatgcg tgcctgatca tggatacctt
     2641 ccacctctac cgcggcggat caggatttga gggcatccgg cacatacgcg gcaatttcat
     2701 tgccaatttc cacatcaacg acgtcccatc cgagcctgcc cgcgaggaga tggtggacaa
     2761 acaccggatc tatcccggcg acggcatcct gcccctcgcg agcgtgtttc agcaactcaa
     2821 ggagatcgac taccggggcg cattgtcgat cgaaatgttc aatgaggagc attacgccca
     2881 ggagccgaag gtggtcgcgg agactgcatt gcgcaaaact ctcgcggtga ttgaggggtc
     2941 gggcgtatag gcgttgcggg gcgcggcggg cgccgcatat ccatcagctt cccgccgatg
     3001 acgggcgcaa cagcgcggcg ccggcgcctc ccaggacggc aacgcccccg gcgagcagaa
     3061 acgaggagct cagcgagccg gacgcatccg ccagcgcgcc cgccacgctg ggccctgccg
     3121 cctggccgat cccgaagaac agggtgatga acccgagcgc cgccggagcc atgcgcgggc
     3181 caagcatatc gccgcatgcc gccgccatga tggcgggaat gctccacgcg gtcagcccga
     3241 ataacacggc ggagacggtg aagccaagcg ggttcggcgc gagcgcgaac aacgtgaatg
     3301 ccgccgtctg gatgccatac acgatgacca gggcccattt gcgtccgagc gcgtcggaga
     3361 ccgcccccca gatcaggccg cacaacacgc tgcaccagcc catgagcatg aagaggcgcc
     3421 cggcttcgga atgggtatag ccgccatcgc tcatcagatg cttcacaaag aacgtgaggt
     3481 aaatgatgta cgcaaacccg aacgcgacgt acaccagccc cagatgccag acggtccatg
     3541 atcgatacac gctggcccag cgcaagggcg ccgcgcgggg cgcgggcggg ggctcgcccg
     3601 gcagggcgcc cagaggcagg gcgcccacat cgtcgggatg gttgcgcagc agcaggctgg
     3661 ccgcggccgc aagaataagg gtgatcgcgc cgagcaggcc ccagcacacg cgccaccccg
     3721 cttcgccgaa atgcgttaga agcggcggca cagacggccc gagaaggata agggcaaagg
     3781 aggagcccga cacgccgacg cccgctgcca gaccgcgccg ccgcggggca aaccacgccg
     3841 ccagcagcgc catgacgggg acattgctcg cgccgctgcc aaggcctgtc acgccgcgcc
     3901 agagcgctgc cgtggccatg ccctccgaaa agcccgtcag gatcatggcc gctccggcca
     3961 gagccaggcc ggccgcaatg accgcgcggg ggccgtatcg tgatgccaat gcgccgccca
     4021 cgaccgacaa caccagatat cccgccagat tcgcggtcgc cagcacgccc gcctgggtgt
     4081 tatccagtcc aagcgcggat tgcatcggcg tcagaaggat gctgtagccg aaccgggcaa
     4141 gtccgagcgc gccgaacacg acacaggtgc tcatcacaag gatgacgagc ccatagtgta
     4201 ttcccagcac atctccgccg cgtttcgagg cgttcttcac aagcgctccc ccccggagaa
     4261 catgacaata acagatgggc cctctccatt gcgagacttc ggagcggcgc tgccgcgcac
     4321 cattgattca ctccggctgc accgacataa tggcgttcta aacacgagtg ccgccagttg
     4381 tggaaggagt gccttgccat gccctgcttt atggttctaa tggccgcgat gctggcggct
     4441 gcacccgaaa ccaccgagcc cgttctggtc gttgccaaca agcacgacga cacgctcagc
     4501 tttatcaatc tggcctcgct ggaggtgatg gagaccatcc cgacggggcc caacccgcat
     4561 gagatggcca tcacgcctga ccagcgcatc atatacctct cgaattacgc gccccccggc
     4621 aacaccatct ccgtgatcga cctcgtgcag cggaaacaca tcaaacaaat cccgacaggc
     4681 gactacacgc gcatccacgg cgcgaccatg gcgcccgacg gcaaacacgc ctatttcacg
     4741 gcgggtcaga ccggatttgt ggtggaggtg gacaccgcca cgcacgaagt cacccgcggc
     4801 atccccaccc acggcacgat ctcgcacatg gtgctggtct cacccgacgg cacgcgcctc
     4861 tacaccgcga acatcacgtc tgagaacgtg tcggtcattg accgggcgtc gggcgaactg
     4921 ctcacccaga ttccatgcgg cccgggcgtg gaggggatgg cgttcacgcc cgacggcaag
     4981 cagctctggg ctctgaacca gaccggaggc tccatcacca tcatcgacac ggcgacgcac
     5041 gcgccgcttg agaccttcgc gtgccccggc atgcccgtgc ggatcctttt caccggggat
     5101 ggagcgcgcg cctatattcc gggctggatc gaagagggtg tgctgatcgt gctcgatgtc
     5161 gcggcgcgga aggagctcgc gcgcgtgcgc gtgggccgct tcgccatcgg cattgtgctt
     5221 tctcccgacg gcacgcgcgc gtttgtcggc tgcgaacaca acgacggcgt tcacgtggtc
     5281 aacaccgaga cgctgaccgt cgaaaaggtg atcgccaccg gcaacggccc cgatcccatg
     5341 atgttgtggt accccccgga gcgttaacgc ggtggttttg aggcttctgc gcagcgcacc
     5401 tttccagtgg gagatgcctt ggagacctcg acatgatttt ggcgatgatt tcgatttgtt
     5461 ttgcggccaa tgatgtcacg cccctgccgg tgcaatggga cctcgcacgc ctggcacagg
     5521 ttcctgcaac ctatcccgcc gatggattct gggcggaagg cgtgcaggcg cttttttatg
     5581 aaggcctccc gctggaaggc cggcccacgc gggtgttcgc gtggctgggc gtgcccgaac
     5641 ggtccgatgg cgaacccgtg cccggcatgg ttctggccca cggaggcggc ggcaccgcct
     5701 acgacgaatg ggtgcgcctc tggaacgccc gcggctatgc cgcaatcgcc atggacatgg
     5761 acggcacggt gccgcgaggc gactacaaaa gccggcctac ccacgaacac agcggcccga
     5821 ccgatttcgg caccgcctcg cccatgatgg gagccgttga ggattactgg atcttccacg
     5881 ccatcgccga catcgcgctg gcccattcgc ttctcgcttc gcaaccgggg gtcgatcccg
     5941 cccggatcgg gctcacgggc atttcatggg gcggatttct gaccaacatt gccgcggcgg
     6001 tggactcccg gtttcaattc gcgatccccg tctacgggtg cgggttcatt aatgagctca
     6061 cctggaaacc cgccttcgaa tccatgcccc ccggcaaggc ggaggaatgg atgcgcctgt
     6121 gggacccgtc cggttacgtg ggcgccatca ccatgccgac cttgtgggtc aatgcgccca
     6181 acgatccgca ttaccacctg ggcgcctaca gccgcaccta tttgcgtgcg ggaggctcga
     6241 aaaccctgag cgtgcagccc gggctcggcc acagccacca ccacggatgg gcgccgaagg
     6301 aaatttacgt atacgccgac agccaggtgc ggggcggcgc gcccctgttg cgcatcgtgg
     6361 ccgaagccga ggaaggcaca actgccctgg ccctgttcga gggcggcacg ccggtaagcg
     6421 ccgaactgat atggacccgc gatgccagca actggagcca gtgcacctgg gaaaccgcgc
     6481 cggccatcct ccaggacggc cgggtgtccg cggaaattcc gagcggttgt gcggcgtact
     6541 tcatcaacct gaccgatgat cgcggcctga ccgccagcac cgtctgccgt acacgcaact
     6601 agccttccca catgcgccgt ggtcagggtt gttcagatgg cggctccacc ggccggggcg
     6661 gtgtctgcgg catgggtctg gggcctctct ggcgcccgtg ctctcctctt cctgcccggc
     6721 catcgcctgc gccgcgccgg agcccaccct ggcgcgggaa gccgtctcgc gcgctctccc
     6781 cgcgctgttc acgcaattgc ttgcgtgccg tctcgatgag ggtgcgcatg cgtttatcga
     6841 agtcgcccat gaaaagatag agccgggctt gttgcgcagg cgtcaacgga acgctggctt
     6901 tttcaaatgc gtcaagccgc gtggcgagcg cttcccggtc tttcgcgatc aattgctcga
     6961 gtttttgatc gatgtcgctc tcaggggcat tcgcgctgac gagcccgcgt agttcgcgca
     7021 tgagttcatg ccgttcccgt tgcaccttga ctccgttttc tcgagcctcg ttatactgcc
     7081 gcaccaaaac caccgtctgt tcgtcggtaa gccccagttc gcgggacagt cttaccatgc
     7141 gtactgtctc caccagcgac cggatgtctg cttcgcgcat gcccgcgagc tgttccataa
     7201 gcgggcccgc ggcgcgcggc ggacgcggcg gcccgtcaag cggttgcgcg agcgcaagcg
     7261 tccccatcac acccagcgtc acaacggcaa caaatagcgg caacgtgtgt ttcatgatct
     7321 agccctccat cgcataggcg acaagcagtt ctcgaaaaac agcctcttcg tctggattaa
     7381 gatcctcaat ccacgtgtca agcggcaggt cttcatacat gccggcggga gcgccatcag
     7441 cggcgtatcg gccatcggcg tccggtgaaa caccggcata ctcactttcc agcgcgtcaa
     7501 gtgcgtccgg atccgctgca attcgttcga gcaggatgga atccagcagg tccgggtcga
     7561 tctcacgcca tttctggatc tctgtcgcta tcggagacac gcgggattcc ggcggtgcct
     7621 ggatcgccag ataggcggcg gccagcacga acgcaaccgc cgccgcgagc atccacggcg
     7681 cgacccccat cacccagccg cgatacggcc gggtctgctg ctcccgcagc ctcgccagca
     7741 gccgcaccgc aaattcgggt ccggcctctg gcgacggcaa gcggcgcaga gcctgagatt
     7801 gcgacaaaag aatgtcgtaa cggcgccgtg ccgccggatc gctctcgagc aggcgttcaa
     7861 ctgcttcggg atccctgctc tcgccgtcga gatacgccga cagcacttcg gagagctggt
     7921 cgttgttcat agatcgcctc ctccgagctc cagccaacgg attcgaaatt gttcgcgtgc
     7981 ccgggccagg cggctccgca ccgtgccttt acgccggcga atcaccgccg cgatggcgtc
     8041 gtagtccagg ccattcagtt cacgcagcac cagaatttcg cggtgctcgg gcgacagcaa
     8101 ctcgagggtg cgttcaagcc gcgcttcgag ctcgctaaga cgcgcctggc tgtccggagt
     8161 gtggagcgga ttgcagaccc gttcctgcac cgcatcgtgg cttgtcagcg gctgggtccg
     8221 gcaccggccg cggcgcttcg catcgcgcag gaaattcaag gtcaggttac gcgccatacc
     8281 gaacaggacg gtcgaaaact tggcctcggg ccgcagtcgc cacaaatacc ggtacacgcg
     8341 aatgaagcat tcctgtgcca ggtcttcggc atcgtcgcgc gacccggtca tgcgatggca
     8401 gaaggcgatc aggccgttct cgtgccgtcg aaccagcgcc ccaaaggcct ggtcatcgcc
     8461 ggcacgcgcg agcgcgacga gttcccagtc cgagggggta ttcatgcgcc ggacaacctc
     8521 gaccacatga gatgaaacac ctcccctgcc aggaagttcc gtagcgccgc gccgcagggg
     8581 acagtggagg gactgaggat gccgctcatt tcgaggaggc gcgttcggct tcctggcgca
     8641 cgcgcaggat gaggcgggtc agttcgtcct gcagggcagc ggcttcttcg agcatggggc
     8701 gcaatgccgg ttcggcggcc atctggatac cgttgagcag gcgcagcaga aaggccattt
     8761 cgcgtgtagc ccggcgcgct cccgcaagct tgttgatctg gtttctcggg gtgctgtgca
     8821 ccagcgcctc agagacgccc gccccgaaat tagtcgccgt gatgataatc cggtcggcaa
     8881 gggcctgggg cagctccggc tcgatctggc gcacgagttc gatgatcgac aaggtcaatt
     8941 gaaacgaccg ctgctcgata tcatgaaggg cctgcatccc ctgcaagcgc gaggtcctca
     9001 tggcgccacc ttcccgcacg ggccacaaac gccccgccgg cgcagaccgt gctgttacgc
     9061 tcttttcacc cgcggaccgt cgggagtatc ctcgacgacc catcccattg ccttcagtgt
     9121 atcgcgaatc tcgtcggcgc gcgcaaagtc tttttcacgg cgggcacgct gccggtcgtt
     9181 cacgagattg agcacatcct gcggcacggc atccgcttct ttcgccggaa acagtgcgca
     9241 cacggcgtcg agccggtcca gaagggccag cgcgttcgcg gctccctccg caccgaggct
     9301 gccatcatcg atccgcttgt tggtatcgcg gacgaaatca aacacggccg ccagggccgc
     9361 ggagatgttg aggtcatcgt ccagggcttc gccaaacgct ttttcacagg ccgcggcctc
     9421 atcggcggga tcggcgccag cgccttcgac ctctgcgagc cgcagccgga aatcctgaat
     9481 gcggcggacg gattgggcgg ccgcttcgag gccattaaat gtgaagttgc tgggctcgcg
     9541 gtaatgcgtt gcggacaggg cccagcgaat ggcgacgggg tcgtacccct tgtcaagcag
     9601 gtcgcgcagg gtgtaggtgt tgccgtacga cttcgccatc ttgcgccctt ccacctgcag
     9661 atggcccgcg tgaagccagt acgagacaaa cggttttccg gtggcggcct cggactgcgc
     9721 gatctcgttt tcgtggtgcg ggaacaccaa atcgaccccg ccgcaatgga tgtcgaacga
     9781 gtccccgagg tgtttgcggc tcatacagga gcattcgatg tgccatcccg gccgcccttt
     9841 tcccagttcc gtgtcccaat acacgtcgcc atcgtcctca tcccaggctt tccagagggc
     9901 gaaatcgcgg gcgtcttcgg tctcgtattc gtccgaatcg atgcggccgc cggcgttggc
     9961 cttcagggtg tcgatgtcga acccgctgag cttgccgtaa ccgctgaacg acgccaaccg
    10021 gaaatagatg ctgccgtcca cctcgtaggt gtgcccccgg tcgcgcagga tcttgatgaa
    10081 atccacaatt tcagggatgg tttccgtcgc ccggggcatg cggtgcgggc gcagaatccg
    10141 gagcgtgtcc aggtcttcaa ggaaggcctg ggcatagcgg cccgtcaacg attcgagcga
    10201 ttccccggtt tcccggcagg tgcggatgat cttgtcttcg acgtccgtca gattcatgac
    10261 atgcgtgacg gcatagcccc ggtattcgag gtggcggcgc aggagatcct caaacaaaaa
    10321 tgtgcgtaag ttgccaatat gggcgtagtt gtagaccgtc ggcccgcagg tgtagatacc
    10381 ggctttgccg gcttccagcg gcacgaacgt gtccttgcgt ttcgtcagcg tgttgtagaa
    10441 gacaatgctc atgtagctcg ctttctttac gggaatatat ccctcgcagc ccgtccccgt
    10501 gcccgggatt ctagcctcat cgatgaaatc aggtcaaacg ggctggcgcc ggccgccgaa
    10561 gcccggcggc cggcgccagc ctcctgtgcg cgggaagcag ggacttcttt ggcttcgttt
    10621 tgaggcctgg aagcgcgttc gcgcacaaaa tatccgggtt tgacgttgcg tatgggccaa
    10681 aagtgcggta cgattaaaag tgaatgcaca aacgggggaa tctccctcag tttgagtttt
    10741 ttcacgggga aatcacgaat acacggcccg ggaaggagta cgatgccatg agacgagccc
    10801 atcagttggt tttacggata attttgggcg gtattatggc tctcggcgcg gcgaatccgg
    10861 ccttgggcgc ggataacggc ggcggtgcgg ctgcagggac gccgccccag gaaatgaacg
    10921 gcctgaagcg gctgcatttt gaggattttg agaaagatcc cgagatcatc gcaaaacagt
    10981 ggcagcccac cgatcccaag gcctgggccg tcgtggaaga cggcacgggc cacgcctacg
    11041 cgctgaagga tgccagtgat tacaccccgc cggtgcggtc gccactcagc atctcgctcc
    11101 ttaaaggctt tgaggtgacc gatttcattc tccaggcgga catgaaacag accgggaaag
    11161 agtacggcca ccgcgatatg tgtgtctttt tcggatacca ggatcctgcg cacttttact
    11221 at
//
LOCUS       DAOPBQ010000045        10838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_5923_length_10838_cov_4.496708, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000045 DAOPBQ010000000
VERSION     DAOPBQ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10838
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5923_length_10838_cov_4.496708"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1787
                     /locus_tag="PKW60_02715"
     CDS             <1..1787
                     /locus_tag="PKW60_02715"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPJ98376.1"
                     /translation="SDTSARRATLVDEREDLQKELDILRGRIKTMKEKLGVAPGTLSA
                     LGPSETEADRGNLSAAEKGKIEAKNRIETLEGVLAKIAGYRQQVKTSPQEPIFDLNIE
                     EDVRTDIEVGIVRQELAQVKSEIAVLEEKYTVEAPPLKALRNSQASLQTRLDRALAEA
                     RGRLLESAEARVRQELAVAQKALQESETQIEQFQRRIEAQTELAVKAAGQQAGIEALQ
                     SEERDLADKIKHIESIIYSIDVESKAPGRISLSSNLFPPARADYGRRKQLMALAFLAS
                     VCAAVAAGLWRELTDKQVRSPQDVAVLTGLPVLATVPHTSLEKPASRHDGSLLQTARE
                     PESTSADQFRRVLTRIIYPPEGSAELNTCLVISPGRGDGKTSLACNLATALAQAGRRV
                     LLVDINARNPKVEEAFGLPLGPGLGDVLFGGRAPNDVVRQTDYANLFVIGPGTQRQVV
                     VGKLASRDMVAFFEQAEQAFDHVIIDTPPALLMADAKLLAPVVDGVVVVVGVGASTTG
                     MLQRCLSDMRQIGANVIGVVLNKVKATRSGYLRRNLHQYYDYLKEGRGNGSALPEIQL
                     EYNEDELDATIVLVDAETDDDAASPTEG"
     gene            1807..2418
                     /locus_tag="PKW60_02720"
     CDS             1807..2418
                     /locus_tag="PKW60_02720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPJ98377.1"
                     /translation="MSLKLPPALVQTRSALAQALGLLARLCRPASRRLAMFVYEAWAR
                     GQIRGRIAPGVQFFGMVTVEGSGDIVIGPGTRLGRRVYLETREGGQIRIGANCVINDG
                     ATLVAYSSITLEDFVMMGEYATIRDANHGTRRGECIRLQPHTAGAVTIGTDAWIGRGA
                     CVLKGVTVGAGAVVGANSVVTRDVEANVIVAGAPARPIGERSE"
     gene            2436..3242
                     /locus_tag="PKW60_02725"
     CDS             2436..3242
                     /locus_tag="PKW60_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HPJ98378.1"
                     /translation="MIQRERAQPLPARDTVEDEIELFNVRISNLSFDELCHWIDVQVG
                     HRTAAYVVTPNIDHVCRLQRDSEFRDAYAGAGLRLVDSAPLMWFAKLFNQPLKEKLSG
                     SDLVPRVSAFAAGKGYRVFLLGAAEGVADEAARRLQAKYPGLEIAGCYSPPFGFDRDA
                     AQNEAVCERVREANADICFVALGSPKQEIWLLRHAGLMGVPAAFAVGAGVDFAAGRVQ
                     RAPVWMQRSGCEWLWRLMREPFRLWRRYLVEDSYFLVILLREIGRRLGRS"
     gene            3296..6526
                     /locus_tag="PKW60_02730"
     CDS             3296..6526
                     /locus_tag="PKW60_02730"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ98379.1"
                     /translation="MSRRLWVVALIVLGAFVALGLAGAGGLFLYRQQQTRQWHAIAEA
                     AFEIGDWDTATRWYAFYLNREREDIAALERYAEASLQQEGSRKSGLAQAAMAYRQILH
                     YEPERQDIQQALFDLQKKLGAWHEVHYLAGRMLEKDPGNVEFKHEQALALYRLGKRSE
                     AVAAYRALVDEGVATPEAYLNLARLLRETNLTEQADDVLSALATRYPDDPSVFVARSD
                     FFLEGRDTDQARQELQRAQALAPDDPEVVMLEARLALVTGDFDAAARAARKVLEGDPS
                     QARMYLVLAQALAASGDKEAGLDVLRNVDPAVRRDSPELFLQLAEQLINVQQLDEARE
                     VIAAFEKVYPDHQSTLNYLEGRVFLIEGNADAAADRLAAVIEQNPAMSPARFFLAVAN
                     LLQGKRKEARGNLEIYLKENPSDESAKRLLAEAMGVEPDPEQIIQAAQAALGNLDARP
                     DTLVSSALALLASALRQGTVASQAGTIEELFTRALDQNPREVRAYGGLVDAYVAADMP
                     AKARDVLRRAAEAGVPSAELARAKANVDLAQGEPGAAWAAFEEAFAAKRPSVEDARQW
                     SRFFTLREQPDLARRVLTVAAEILTAEPERAELAVESLMLELRSGEDEAAMRLLGAVE
                     SQVAGHPAAERQLQGAKGQLVWRLMELNDARKIEEARQILQQGSGRHPDDPMLFTLQG
                     IMALRQAPPDYEKAQSAFQKALEIDSDSVAALMGMASVTASHGALSQAMEFATKAAAI
                     APKSEGIDLWRAELLFRMQPYLEARNLLENILAEVPGSPAALELLVNTYLATNQLREA
                     ERTLQHLETRLGPEASHVEMIKTLRGRILLAQGNATGAEDVLRAQLAANPDDFSVVRS
                     LAHALAQQRRNEEAEILLRNYAEAHQSDAEAFVSLARFYLGMHDPAKVDAASTALTRA
                     LLLDNDYLPALRAMVDVQLRRGSTAGILAACDRYLTRDPVNAGVLFAKASALSREPAR
                     EQEALELLGQVIALERQPEYLALRGMLYLREKNYAAALSDLRAASLEAPDTSADFDAA
                     LAETYWGLGEKELAKTHYESALAKLQGSDPAPPWRAHLEKLMAQEN"
     gene            6530..7453
                     /locus_tag="PKW60_02735"
     CDS             6530..7453
                     /locus_tag="PKW60_02735"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HPJ98380.1"
                     /translation="MMQESQTPAAPVQAPAAGTEPLSFRSLFELKDLVVVALLAATYR
                     NVAHELRAGWTLVDSYYSHGFLIPFISLFFVWRERKSLASLPRNPSAWGYPWLAAAAT
                     MLFVGDFLGFGVLTHMSLLPMITGVLLLTHGVDRTRTIWFPIAFLFFMIPIPASITQS
                     LALRLKLIATESAVQLANLFTLPMVREGSYVHFGDDFLLVGEVCGGLRSMIALLALGA
                     LMAYISKTRWLARMFLFLIAAPLVAILTNILRIFSLCVVGYFYGSTVAAGTFHDVSGI
                     LIFLTAFICFFTLEGLLRKTLPARENRGTAS"
     gene            7450..8076
                     /locus_tag="PKW60_02740"
     CDS             7450..8076
                     /locus_tag="PKW60_02740"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HPJ98381.1"
                     /translation="MKHYLALVLILALITAGHLAVLKAHATASAALPAVETLDIPDQI
                     GDYSRRGADAEIEDHVKQVLGTSMILIRDYVAPPQKFVQLTVVHSTSRRSLHFPEVCL
                     VGGGWEIMTQTTHPVGFFFNAKKLVLVNGDMRQLVLYWFKTGDELTGNFFVNAYHWAK
                     AQLAFGSPTSTMIKVSTLLRPGQSEEAAFETLEDFAVKLTPILQERIP"
     gene            8142..>10838
                     /locus_tag="PKW60_02745"
     CDS             8142..>10838
                     /locus_tag="PKW60_02745"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ98382.1"
                     /translation="MRSDSTARRMSALGTMALLCLLLAVIPACGGRRSQQQTELGQTF
                     LSIGKSDEALLAFEKALKMNPHNTDAELGMARCFAVQDNLEEALTHYRRVIELDPGKE
                     TAYVEAVRILLRKDRRGDAENLAMDFEKVHPDKGGILHAFIMQTAGQTADAIALLEGL
                     AAKYPASTDVRVSLASALAAAGQDAKAVETLEDVLANVDPDSLAARMKLVEVYQKQGK
                     TDEIVTQFREMANQKPGDLGIQLALARSLVAKKEYAEAEDIARAILRELPESGWANYV
                     VGACLLAREEYEKAVMYLQTATQALPESEAVRDLLAVARRGGAPLAQAPETAASTAPQ
                     SPAPGEAHPQSAGDLPWPVLWKQARFDLLLQQSDALLSHDEPNLRETLVLAALFSGDP
                     SDPPRAKQFAEGLPQDSPYRALLAALEARDPEAALAVFDAWTETEPERVVLRGNAYGF
                     TLMLVGARAKALQELSECYAAAPDNAVALFNLATMYRTARLPEYAVQVLDRFLALYPD
                     NLGARRTLLRLLVDANEMSRARAIAEFTYQLYPHDAEAVVDLASVYRVTGEVDLAQSL
                     LTHAQDADPDSAPLRVAEARLNLLQGRDDAVLQSLEGRAFDSVEHEAARESLRAFALA
                     ENNDWSEVTAICEALPSQKRSAGLHLLSAAAYLHGDKPELARGALDGASKASWERFSG
                     AAVALAALGILDPPKGSETLVAQLTAAPEGLAAYVHGVACLAESLPDCAVGPLEKASA
                     AAPGSASLAALMLEALTNTIRFGDPAPKAQEVAQRAPADPRTWLALAAFCHAKTDTAG
                     ENAAIQKALELDGNNAAAWLLRARYLESAGDKAGALAAYERVHATRPTDPVVGNNLAY
                     LILETGGDPQRALQLAHAALDAAQNNPAIQPNVLHTLGLAELR"
BASE COUNT         2026 a         3341 c         3487 g         1984 t
ORIGIN      
        1 tcagcgacac cagcgcccgg agagccaccc tggttgacga aagggaggat ctgcagaagg
       61 agctggatat tctgcggggc cggatcaaga ccatgaagga aaagctgggc gtcgcgccgg
      121 gcacgctttc cgctctgggt cccagcgaaa ccgaggcaga ccgcggaaac ctctccgcag
      181 ccgaaaaggg caagatcgag gcgaaaaacc gcattgagac actggaaggg gtcctggcca
      241 agatcgccgg ctaccggcag caggtaaaga cctcgcccca ggagccgatc ttcgacctga
      301 acatcgagga ggatgtgcgg accgacatcg aggtaggtat cgtccgccaa gaactggccc
      361 aggtgaagag cgaaatcgca gtgctggaag agaaatacac ggttgaggct ccgccgctga
      421 aggcgctgcg caactcgcag gcttcgctcc agacccgcct cgatcgggcg ctcgccgagg
      481 cgcgcggacg gttgctcgag tccgctgaag cgcgtgtccg gcaggagctg gccgtcgcgc
      541 agaaggccct tcaggagtcc gaaacgcaga tcgaacagtt tcagcgccgt attgaggccc
      601 agaccgagct ggcggtcaag gccgccggcc agcaggccgg gatcgaggcc ctgcagtccg
      661 aagaaagaga cctggccgac aagatcaagc acatcgagtc gatcatttac agcatcgacg
      721 tcgaaagcaa ggcccccggc cgaatttccc tcagttccaa tctgtttcca cccgcccgcg
      781 cggattacgg ccgccggaag cagctgatgg cgcttgcctt cctggcctcc gtctgcgccg
      841 ccgtagcggc gggcctgtgg cgggaactaa ccgacaagca agtgcgcagt ccccaggacg
      901 tggccgtttt gacgggcctg ccggtgctgg ccaccgtgcc gcataccagc ctggaaaagc
      961 cggcctcgcg ccacgacggc agcctgctgc agaccgcccg agagccggag tctacgtctg
     1021 cggaccagtt ccgccgcgtc ctcacccgga tcatctaccc gccggaaggg tctgccgagc
     1081 tcaacacctg cctggtgatc agccccggcc ggggcgatgg aaagacctcg ctggcgtgca
     1141 atctggctac ggcgctggcc caggccgggc gccgggtcct gctggttgat atcaatgcgc
     1201 gcaacccgaa ggtcgaggaa gccttcggcc tgccgctcgg ccccggtctt ggtgatgtgc
     1261 tgtttggcgg ccgtgcgccc aatgacgttg ttcggcaaac cgactacgcc aacctcttcg
     1321 tcatcggccc cgggacgcag cgccaggtgg ttgtgggcaa gctggcgtcg cgggatatgg
     1381 tggccttctt tgagcaggcc gaacaggcat ttgatcacgt catcatcgat accccgcccg
     1441 ccctgctgat ggcggacgcg aaactccttg cgcccgtggt cgatggcgtg gtggtggtgg
     1501 tgggcgtggg cgcctccacg accggcatgc tccagcggtg cctgagcgac atgaggcaga
     1561 tcggggccaa cgtgatcggc gttgttctca acaaggtcaa ggcaacgcgg agcggatatt
     1621 tgcgccgcaa tctccatcag tactacgact acctgaaaga agggcggggc aatgggtccg
     1681 cgcttcctga aattcagctg gaatataacg aggacgagtt ggacgcaacc atcgtcctgg
     1741 tggatgcgga gactgacgac gacgccgcat ctccgaccga gggatgatgg cccgccggtg
     1801 ccagccatga gcctcaagct gcctccagcg ctggttcaaa ctcgctcggc cctggcgcag
     1861 gcgctgggtt tactggcgcg cctgtgccgg cccgcaagcc ggcggctggc gatgtttgtg
     1921 tatgaggcgt gggcccgtgg gcagatacgc gggcgcattg ccccgggcgt gcagtttttc
     1981 ggcatggtga cggtcgaagg ctctggggat atcgtgatcg gcccgggaac ccgtctggga
     2041 aggcgggtgt acctggaaac ccgggaaggc ggccagatcc ggattggcgc caattgcgtc
     2101 atcaacgacg gagcaacgct cgtcgcctat agcagcatca cgctggaaga tttcgtcatg
     2161 atgggcgaat acgccacgat tcgcgacgcc aatcacggca cccggcgcgg agaatgcatc
     2221 cgcctgcagc cgcacaccgc gggcgcggtc acgataggga ctgatgcctg gattggccgg
     2281 ggcgcgtgcg ttttgaaagg cgtgaccgtc ggcgcaggcg cggtggttgg cgccaacagc
     2341 gttgtaacgc gggatgtgga ggcaaatgtg atagtagcgg gcgcccccgc gcgccccatc
     2401 ggagagcgat ccgaataatg tcaatggaac gaatcatgat tcaaagagaa cgggcccagc
     2461 cattacctgc gcgcgatacc gtggaagacg aaattgagct gtttaatgtg cgcatctcca
     2521 acctgtcgtt tgacgagctt tgccattgga tagatgtgca ggtcgggcac aggacggccg
     2581 cctacgtggt gacgccgaat attgatcatg tctgccggtt gcagcgcgac agtgaattcc
     2641 gggatgccta cgcgggcgct ggcttgcgcc tggtggacag cgcgccgctg atgtggtttg
     2701 cgaaactctt caaccagccg ttgaaagaaa agctgagcgg ttccgacctg gtgccgcgcg
     2761 tgtctgcgtt tgccgccggg aaaggctatc gcgtgttcct gctcggtgcg gcggaaggtg
     2821 tcgctgacga ggcggcgcgc aggctgcagg caaagtatcc cggcctcgag attgcgggat
     2881 gttacagccc gccgttcggc ttcgaccgcg atgccgccca gaacgaggcg gtctgtgagc
     2941 gtgtgcgtga ggccaacgcg gacatttgct ttgtggcgct cggttctccc aaacaagaga
     3001 tctggctgct gcggcatgcc gggctgatgg gtgttccagc ggcgtttgcc gtaggcgcgg
     3061 gtgtagattt cgcagccggg cgcgtgcagc gggcgcccgt ctggatgcaa cgttccgggt
     3121 gtgaatggtt gtggcgcctg atgcgggaac ccttccgctt gtggcgccgc tatctggtag
     3181 aggacagcta tttcctggtc attctgctca gagaaatcgg ccgccgtctg ggccggagct
     3241 gatgcagcga gccgatgtgc ggcggagagc cggagcaccc ggaaaggaga cggtcttgtc
     3301 acgcagattg tgggtagtcg cgctgatcgt gcttggtgcg tttgtggctc tcggcctggc
     3361 tggcgcgggc ggcctgtttc tttatcgcca gcagcagacc cggcaatggc acgcgatagc
     3421 ggaagcggcg tttgagatag gcgattggga taccgctacc cgctggtatg cgttctacct
     3481 caaccgggaa cgtgaagata ttgctgccct cgaacggtat gcggaagcga gcctgcaaca
     3541 ggaaggcagc cgcaaaagcg gtttggcgca ggcggccatg gcgtaccgac agatccttca
     3601 ctatgagcct gaacgccagg atattcagca ggcgctattc gacctccaga aaaagctcgg
     3661 cgcctggcac gaggtccatt atctcgcggg gcgaatgctg gagaaggacc cgggcaatgt
     3721 ggaattcaag cacgagcagg ccctggcgct gtaccgcctg ggaaaacgca gcgaggccgt
     3781 cgcggcctac cgcgcactgg ttgacgaggg ggtggccacc ccggaagcct acctcaatct
     3841 ggcacggctt ctgcgggaaa ccaatcttac ggagcaggcc gacgatgtgc tcagcgccct
     3901 ggcaacgcgg tatcccgatg acccctctgt gttcgtagca cgttccgatt tttttcttga
     3961 agggcgcgat acggaccagg cccggcagga gttgcagcgg gcacaagcac tggcgccgga
     4021 cgacccggag gttgtcatgc tggaggcacg gctcgcgttg gtaacgggcg attttgacgc
     4081 tgccgcgcgc gcggcgcgca aggttctcga aggcgatccc agccaggccc gcatgtatct
     4141 ggtgcttgcc caggcgcttg ccgccagcgg cgacaaagag gccgggctgg atgtcctgcg
     4201 caatgtggac ccggccgtgc ggcgagacag cccggagctg ttcctgcagc tcgcggaaca
     4261 gctgatcaat gtgcagcagc tggacgaagc ccgcgaggtc atcgccgcat ttgaaaaggt
     4321 ttatcctgat catcagtcca cactcaatta tctcgagggc cgggtgtttc tgatagaagg
     4381 caatgccgat gccgcggccg atcgcctggc ggccgtgatc gagcaaaacc ccgcgatgtc
     4441 tccggcgcgt ttcttcctgg ctgtggccaa tctcctccag ggaaaacgca aggaagcccg
     4501 gggcaatctc gaaatctatc tgaaggaaaa ccccagcgac gagagtgcga agcgactgtt
     4561 ggccgaggct atgggcgtgg agcctgaccc ggaacagatt attcaggcgg cccaggcggc
     4621 tctcggcaat ctggacgcgc ggccggacac cctggtctcg tcggccctcg ctctgctggc
     4681 ttctgccctt cgccagggca cggtagcgtc tcaggccggc accattgaag aactgtttac
     4741 ccgcgcgctt gaccagaacc cccgggaagt gcgtgcttac ggaggccttg tggatgccta
     4801 tgtggctgcg gacatgccgg ccaaagcccg cgacgttctc cggcgcgcgg cagaggccgg
     4861 ggtgccttcc gctgaattgg cccgcgccaa agccaacgtg gatctggccc aaggcgagcc
     4921 cggggcggca tgggcagcct ttgaggaagc gttcgccgct aaacggccct ccgtcgaaga
     4981 cgcgcgccag tggtcccggt tttttacact gcgcgaacag cctgacctgg cgcgccgggt
     5041 cctgacggtg gctgccgaaa ttctgaccgc cgaaccggag cgcgccgaac tcgccgtgga
     5101 aagcctgatg ctggaactgc ggtcgggcga ggatgaagcc gccatgcgtc ttttaggcgc
     5161 cgtcgaatcg caggtggcag gccatccggc cgcggaacgg caactgcagg gcgcaaaagg
     5221 gcagctggtc tggcgcctga tggaactgaa cgacgcgcgc aaaatcgaag aagcccgcca
     5281 gatacttcag cagggctccg gcaggcatcc ggacgatccc atgctcttta ccctgcaagg
     5341 catcatggcg ctccgccagg cgccgccaga ctatgaaaag gcgcaatccg cctttcagaa
     5401 agcgctggaa atcgacagcg acagcgtcgc tgcgctcatg ggcatggcaa gcgtgaccgc
     5461 ctcgcacggg gccctttctc aggccatgga attcgccacg aaagccgcgg cgattgctcc
     5521 gaaatccgag ggcattgacc tgtggcgggc tgaactgctg tttcgtatgc agccgtacct
     5581 cgaagcgcgc aacctgcttg aaaacatcct cgccgaggtg ccgggcagcc ccgcggccct
     5641 ggaactgctc gtcaacacct atctggctac caaccagctc cgcgaggccg aacggaccct
     5701 gcaacacctg gaaacgcggc tcggtccgga agcgagtcac gtcgagatga tcaaaaccct
     5761 tcgcgggcgg atcctgctgg cgcaaggaaa cgcgactggc gcggaagatg tactgcgggc
     5821 ccagctggca gcgaatccag acgatttctc cgtcgttcgc agcctggctc atgcgcttgc
     5881 gcaacagcgg cgtaacgaag aagccgagat cctgctccgc aattacgccg aggcccatca
     5941 atccgatgcc gaagcgtttg tgtcgctggc gcgcttctat ctgggcatgc atgatcccgc
     6001 gaaggttgac gccgcatcaa ccgcgttaac ccgcgctcta ctgctcgaca atgattatct
     6061 gccggcgctt cgcgccatgg tggacgtgca gttgcggcgg ggcagcacgg cgggaattct
     6121 cgcagcatgc gaccgctacc tgacccgcga tccagtcaac gccggcgtgt tgtttgccaa
     6181 ggcctcggcg ctgagccgtg agccggcccg ggaacaggag gcgcttgaac tgctcggcca
     6241 ggtgatcgcc ctggagcgcc aaccggagta cctggcgctg cgcggcatgc tgtatttgcg
     6301 cgaaaaaaat tatgctgccg ccttgagcga cttgcgggcc gcttccctgg aagcgcccga
     6361 tacgtctgcc gatttcgatg ccgcactcgc cgaaacgtat tgggggctgg gcgagaagga
     6421 actcgcgaaa acccactacg agagcgcgct cgcgaaattg cagggcagtg atcccgcgcc
     6481 gccctggcgg gcgcatcttg aaaaactgat ggcccaggag aactagctga tgatgcagga
     6541 atcgcagact ccggctgcgc cggtccaagc ccctgccgcg ggaaccgaac ctctttcgtt
     6601 ccgttcgtta tttgagctca aagacctagt ggtcgtggcg ttgctggccg ctacttaccg
     6661 aaacgttgcg catgaactcc gcgccggctg gacgctcgtg gattcctatt actcgcatgg
     6721 gtttttgatc ccgttcattt cgcttttctt tgtctggcgc gagcggaaga gcctcgcgtc
     6781 gcttccccgc aacccttccg cctggggata cccctggttg gccgcagcgg ccacgatgct
     6841 gtttgtcgga gattttctcg gcttcggcgt gctgacgcac atgtcattgc ttccgatgat
     6901 aacgggcgta ttgctgctta cgcacggcgt ggaccgtacc cgcacgatct ggttccccat
     6961 cgcgtttctg tttttcatga tacccatccc ggcgtctatc acgcagagcc tcgcgctgcg
     7021 gctcaagctg atcgccacgg aatccgcggt tcagctcgcg aatctgttca cgcttcccat
     7081 ggttcgcgaa ggatcgtacg tgcattttgg agacgatttc cttctggtgg gcgaggtgtg
     7141 cggcgggctc cgctcgatga tcgccctgct ggctttgggc gcgctgatgg cctatatcag
     7201 caaaactcgt tggcttgccc gcatgtttct gtttctgatc gcggcgccgc tcgtggccat
     7261 cctcaccaac attctgcgca ttttcagcct ctgcgttgtg gggtattttt atgggagtac
     7321 cgtggccgcg ggcacattcc acgacgtctc cggcatcctg atttttctga cggccttcat
     7381 ctgcttcttc acgctcgaag ggctgctgcg caaaaccctg cccgccaggg aaaatcgggg
     7441 gaccgcctca tgaagcatta tctggccctg gtgctcatcc tcgcgctgat cacggcggga
     7501 catctggctg ttctcaaggc gcatgccacc gccagcgctg ccctgcctgc cgtcgaaaca
     7561 ctggacattc ccgatcagat cggcgactac tcccggcgcg gcgccgacgc ggaaatcgag
     7621 gatcatgtca aacaggtgct cggcacgagc atgatcctga tccgggatta cgtggcgcct
     7681 ccgcagaaat ttgttcaact gaccgtggtc cacagcacgt cgcgccgaag cctccatttc
     7741 ccggaagtgt gcctggtcgg cggcggctgg gaaattatga cgcagaccac tcaccccgtc
     7801 gggtttttct tcaacgccaa gaaactggtg ctcgtaaatg gcgacatgcg ccagctggtc
     7861 ctctactggt tcaagaccgg ggacgaactc accggcaact tttttgtaaa cgcctatcat
     7921 tgggccaagg cccaactggc gtttggctcg cccacctcaa ccatgatcaa ggtgtccacg
     7981 ctgctccgcc cgggtcagag cgaggaggca gcgtttgaga ctctggaaga ttttgccgtc
     8041 aaactcacgc ccattctaca ggagcggatt ccgtagaacg ggccggtgca cgccgccgca
     8101 tcccgaccgg gtcggcgaag cagtcagaaa agggagacgt tatgagatcg gattccactg
     8161 cccggagaat gagcgctctc ggtacgatgg cactcctgtg cctgctgctc gcggtgatcc
     8221 ccgcgtgcgg cggccgccga agccagcaac agaccgagct ggggcagacc ttcctcagca
     8281 tcgggaagag cgacgaggcg ttgctggctt ttgaaaaagc cctcaagatg aatccgcaca
     8341 ataccgatgc cgaactgggt atggcgcgct gttttgcggt tcaggacaac ctggaagagg
     8401 ccctgacgca ttaccgccga gtcatcgagc tcgatccggg caaagaaacg gcttatgtcg
     8461 aagccgtacg gatcctgctg cgaaaagacc ggcgtggcga tgcagaaaac ctggccatgg
     8521 atttcgagaa ggtccatccg gacaaaggcg ggatactgca cgcgtttatc atgcaaaccg
     8581 ccggccagac cgcggacgcc atcgccctgc tggaaggcct ggcggcgaaa taccccgcct
     8641 cgacggatgt gcgggtcagc ctggcctccg ccctggcagc cgcgggccag gatgcaaaag
     8701 ccgttgaaac cctcgaagac gtgctcgcca atgttgaccc ggattcactg gccgcccgca
     8761 tgaaattggt ggaggtctat cagaagcaag gaaagaccga cgagatcgtc actcagtttc
     8821 gggaaatggc aaatcagaaa cccggcgacc tgggcatcca gcttgcgctc gcgcgcagtc
     8881 tcgtcgctaa aaaggaatat gcggaggcgg aggacattgc ccgcgccatt ttgcgcgaac
     8941 tgcccgaatc gggctgggcc aattatgtgg tgggtgcctg cctgcttgcc cgcgaagaat
     9001 acgaaaaggc cgtgatgtat ctccagaccg ccacgcaggc cctcccggaa tctgaggcgg
     9061 tgcgggatct tctggcggtc gcgcgacggg gaggcgcacc cctggctcag gcgccagaga
     9121 ccgcggcttc gactgcaccg caatctcctg ccccggggga agcccacccg caatcggccg
     9181 gagatttgcc ctggccggtc ctgtggaagc aggcgcgttt tgatttgctg ctacagcaga
     9241 gcgatgcgct gctgtcccat gatgagccga atctgcgtga gactctcgtg ctggctgccc
     9301 tcttctcggg cgatccgagc gatccacccc gcgcgaagca attcgccgaa ggtttgccgc
     9361 aggactcgcc ctaccgggcc ctgctggcgg ccctggaagc ccgggacccc gaggcagccc
     9421 ttgccgtatt cgatgcgtgg acggaaactg agccggaacg tgtcgttctt cgcggcaacg
     9481 cgtacgggtt taccctcatg ctcgtgggcg cccgggccaa agcgctccag gaactgtcgg
     9541 aatgctatgc cgcggcgccc gataacgccg tcgcgctgtt caacctcgct accatgtacc
     9601 gcaccgcgcg cttgccggag tacgccgtac aggttctgga cagatttctg gccctgtatc
     9661 ccgacaatct gggcgcccgg cgcaccctgc tgcgcctgct ggtcgatgcc aacgagatga
     9721 gccgggcccg cgccatcgca gaattcacgt atcagctgta tccccatgat gccgaggccg
     9781 tggttgacct cgccagcgtg taccgcgtca ccggcgaggt ggaccttgct cagagtctcc
     9841 tgacacacgc ccaggatgcc gaccccgata gcgcgccctt gcgcgtggct gaagcacgcc
     9901 tcaatctgtt gcaaggccgg gacgatgccg ttttgcagtc gctcgagggc cgtgcatttg
     9961 attcggtaga gcacgaggcc gcccgggaga gcttacgggc tttcgcgctg gcggaaaata
    10021 atgattggtc cgaggtaacc gcgatttgcg aggcccttcc gtcgcaaaag cgctcggccg
    10081 gcctccatct gttgagtgct gctgcctatc ttcacgggga caaacctgaa ttggcgcgtg
    10141 gtgcgctgga cggagctagt aaagcgtcct gggagcgctt ctccggtgcg gcggtggctc
    10201 tggcggcgct cggaatactc gacccgccga aagggtcaga gacacttgtg gcgcagctca
    10261 ctgcggctcc tgaggggctg gcagcctacg tgcatggcgt cgcatgcctg gccgaatccc
    10321 tgcccgactg cgccgtggga ccgttggaaa aagcgagcgc ggctgcgccc ggctcggcct
    10381 cgctggcggc tctgatgctg gaggcgctga cgaacacgat ccggttcggc gatcctgcgc
    10441 cgaaagcaca ggaagtggcg caacgggccc cggccgaccc gcgcacctgg ctggctctgg
    10501 ccgcattctg tcatgccaaa acggataccg ccggtgaaaa tgcagccatc cagaaggcac
    10561 tcgaactcga tgggaacaac gcggcggcgt ggcttcttcg tgcgcggtat cttgaaagcg
    10621 ccggagacaa ggccggtgcg ctggctgcct atgagcgcgt gcacgcaaca cgccccaccg
    10681 acccggttgt gggcaataat ctggcctacc tgatactcga gaccggcggc gatccgcagc
    10741 gtgccctcca actggctcac gccgccctgg acgcggcgca gaataacccg gccattcagc
    10801 ccaatgtcct gcataccctg gggctcgcgg agctgcgg
//
LOCUS       DAOPBQ010000046        10758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_5974_length_10758_cov_4.321499, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000046 DAOPBQ010000000
VERSION     DAOPBQ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10758
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5974_length_10758_cov_4.321499"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            349..831
                     /locus_tag="PKW60_02750"
     CDS             349..831
                     /locus_tag="PKW60_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HPJ98383.1"
                     /translation="MVTESDSTESRILHVLASEGLWLVTAESCTGGLLAHRIVSVAGA
                     SECFLGGVVAYSNALKTDLLGVSPTTLSTAGAVSPEVAREMAEGARDRFHADLSVAVT
                     GVAGPGGGSVEKPVGLVFIAVAWNGGCDVARYEFQGARHAVRQQAADAALAMLWERIQ
                     "
     gene            828..4289
                     /locus_tag="PKW60_02755"
     CDS             828..4289
                     /locus_tag="PKW60_02755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPJ98384.1"
                     /translation="MSGGFCHLHVHTEYSVLDGAIKVSELLDRCQDYGMRACAISDHG
                     AMFGVVDFYQTAKSKGIKPIIGSELYIAPGHRTDRSSKTQSAAYNHLLLLCKNETGYR
                     NLCKLSSIGYLEGFHYKPRIDFESLSRHAEGLIASTGCLRGVVPQYLLREEFDEAEEA
                     LQRYLALFGAENFFVELQDHGLPEQRRVNPRLVDMARRSGLMLAATNDCHYLNKEDAE
                     AHDALLCVQTNRTLEEENRFRFQTKEFHFKSPEEMRETFAAWPEAITNTEKIADLCEL
                     EIPLHKHLIPKYSPPPGKSKPELLRELVMEGLRERYHGKPAPQYVKRAEYELGVIEQM
                     GFVDYFLVVWDLIKTARDKGIPVGPGRGSGAGSLVAYALTITNIDPLRYNLLFERFLN
                     PDRVSMPDFDLDFCYTRRPELIEYVREKYGQKNVSQIITFGRMLAKQVIRNVGRVMGM
                     NYGDVDRIAKLVPDELKITLKDALEKEPELKRQVKEDPQVARLWSLATRLEGTIGTLG
                     THAAGVIICDEPLTEHVPLFQASGSDVVATQFEMKTAEQIGLLKMDFLGLRTLTVVHD
                     AVRFIEENRGVTIDIDSLEPDDSLTYELLRSGRTTGIFQLESSGMRDLSRRIGLESLE
                     EVCALVALYRPGPMQLKDQYIENKHNPAKLSYDHPLLEPILKETYGVALYQEQVMQIV
                     QALAGFSLGQADELRRAMAKKKADLMEQQGVQFLKGAKENGIDTATATSLFQKIEQFA
                     GYGFNKSHSMAYAFVAYQTAFLKANYPVEFMAALLTSESGNLDKVAQYIEECRRMGIE
                     ILPPDVNHSCYGFNVEGSAIRFGMGAVKNVGRDPVEAIQAEREANGPYADIFDFCARL
                     DSRVLNRRVLESLNRAGAFESTGWNRRQVEESLESALSEGQINQRERAAGQTSLLELM
                     AGASDEPLIHKRPDVEEWPENQLLAQEKEVLGLYVSSHPLARHATLLERFSTITLADL
                     PSLAEGHEIRIGGLVSNAKIHVTQRNRKMAFVTLETLESPCEVVVFPELYEQKAELLV
                     PDMVVMLRAKVSYRNDEVSLLAEDIVPIDDTEKRFTEAVHIRLSTVGLDEALLTRLAE
                     LLDAQSGSCDVFLHCVTPERDEIIVHATPSCMVAPSERLCRELEELLGEGCLWYSGAN
                     GFPRHAR"
     gene            4535..5008
                     /gene="fliW"
                     /locus_tag="PKW60_02760"
     CDS             4535..5008
                     /gene="fliW"
                     /locus_tag="PKW60_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HPJ98385.1"
                     /translation="MQLETVRFGTLEVAPDAIITFTQPILGFQEYRRFVQLPGPSDYL
                     RWLQSTESSELAFIMMVPTVLVPDYHVDVRPAELSELAVSSIEELEVYTLVVVPEDKS
                     KVRTNLRAPILINPRLRLAKQALLDRSDYPIQFFLAPPHQGQGQAREASHAGLNP"
     gene            4989..5210
                     /gene="csrA"
                     /locus_tag="PKW60_02765"
     CDS             4989..5210
                     /gene="csrA"
                     /locus_tag="PKW60_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010197004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HPJ98386.1"
                     /translation="MLVLTRKEDESIMIGDDIEVKVLDLKESQVKLGIVAPKSVAVHR
                     REVYLAIQSENTQAAAAAQIDALTDLIQR"
     gene            5231..5857
                     /locus_tag="PKW60_02770"
     CDS             5231..5857
                     /locus_tag="PKW60_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98387.1"
                     /translation="MELIVDGNIRSLGEETPASVFDLVVSVGRQLQEEGRSIMTVQID
                     GQEISAGLLVETMMNKPAASARQIVITSADTRALVNESLSDMQTSLPDLPLACQELAR
                     VFQSETPDAGYEPFQQLASLWSTIKERELMVANALEISLEELQIDGLSVKQFHDELNA
                     FLREATEALKKRDSILLADLLEYELAPRAEKESAIVAILQEKAEQLAG"
     gene            5860..9198
                     /locus_tag="PKW60_02775"
     CDS             5860..9198
                     /locus_tag="PKW60_02775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPJ98388.1"
                     /translation="MAAPRILTFNFHEPYLCLMARTGMPFVIGEYDKPPLARKWQEHY
                     RPIPAGFTFLDESAWRQELAEGRFDLVIAQNEMNAATLSKEVLLSATPLLVLCHNRRT
                     FLETTARVNPDQPHNTFQRLLETLREFARFVFISESKRDDYGFQGPVIRPGIDVEEYG
                     GYTGERLGVLRVGNAMRPRDAMFDVDFQEQVCAGFPNHVAGDNPDIPGSQPAPTFEHL
                     RELYRTFRCYLHVTREAFEDGYNLALLEAMATGMPVVSLANPTSPLTDGKDGFLSYDP
                     DVLRGHIETFLNDLDYAREIGARARETVAKAFPMARFVEAWRETILSAAEFSPRKSAR
                     SPRPAPPAPSPTRPAPAANILLHYMASPITTGRYFEAALRKHHHVVTAGLRCPEAILE
                     RWGFSGEAPPYASHDIELGLEGTCSELLAQLPEGFEPSLYLWVDSGPKEAPPDLHSLR
                     IPKACYLIDTHLAPELRMAIARQFDIAFLAQKAQIPMFQEAGIASVRWLPLACSPELH
                     DVGQLERIYDVAYIGRIQEDPHDRRASILDCVRSRFPNHCIGQQWPEDMARTYAQSKI
                     VVNAAVNHDLNMRVFEAMASGALLVTDQADGLSDLFQEGEHFVLYRDEVELCALIERY
                     LNDPDARERIAKQGQELVLAQHTYDHRMEQLLEMVSEATRMPGGNEGESRYHYGGYYC
                     SPRSELAAHIPPDTKRVLDIGCGAGAFGHSLKQRGVDEVIGIEIDERASALAEEVLDG
                     VLTGDIERMELPFESAYFDCITLGDVLEHLIEPAAALRRLAPFLAPEGVVVASIPNAR
                     FCQVVRMLAEGRWKYEDAGILDRTHLRFFTAVEMQLLFQGAGYEILKMQPLSMLPAES
                     LPRNDDGGFTMGRLTIGPLDDVEYQDFLVFQYLVVAKRAPGSPLERARAALTADRFEE
                     ACTIAALAGDADPTERNFILGQALGRMGRFAAAEAALREALASSPDRADITGQLGVLL
                     VAMNRHEEAIPCLAQATAADPNDFRALGAWGLALLAAGRLEEAFEKLKASLDVHFDNI
                     SLMEPFVSLGESQERWEDLEPVLRRFVEFYPGNSAMALRLARILVLLGKTGEAREHLE
                     TVLLFDAGNADASRLLEEIARGGA"
     gene            9250..>10758
                     /locus_tag="PKW60_02780"
     CDS             9250..>10758
                     /locus_tag="PKW60_02780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPJ98389.1"
                     /translation="MMKPAREWAAELADVVPPPGVCLEEGREGEPTLRVGKAYLHSRY
                     KPREDARRLLDSADLDPARPVLVVGVGLGYHILELCERGYRVAAVEPERAVAALAVQG
                     PLRDSEVLLGLGEPDAIAACPRFQQFARETPQVFVHPPSARLQPAFAEAMAAQISRAA
                     LLGQRLRIAVVGPMFGGSLPIADYLARAFQKLGHQTIYADNSLAWNLYETMTTSVKSR
                     KAASQLGELLVHFLGEWSYARVMEFGPDICIVMAQAPVAPQFPLRLAPAGIVTAFWYV
                     ENWRHLPYWKELAPRYDHFFHIQPGAFEDKLREIGCLHPACVLTGCDPDIHRPVTLEE
                     TERDEFSCDVSFAGAGYMNRNQLLSGLTDYALKIWGVNWTHPSLRGVIQRPNERFTPE
                     LFARIVAGSKINLNLHSSTSFPGVDPHCDAINPRVFEIAACGGFQLCDACKGLDACFD
                     FDSELPVYTSLRELRHKLDHYLAHPAERTQIAAAARERALRDHTYEKRAAQML"
BASE COUNT         2195 a         3335 c         3201 g         2027 t
ORIGIN      
        1 cgcccgcggg ggacaggtcc cctgggtgcg ccgacgtcct cgtcggcaca cggctcagcc
       61 ccgcccgcgg ggccgggggt ggaggcgccg gccggcccga ggcgggagaa cacgccccag
      121 gccggttcaa agcagctgaa atccgctctc ctggcatcgc ctcgccttcc atgtttcggt
      181 aagatcgcaa atgacaagac gataccctcc tcgcaaagat cggagatggc gcgattcgga
      241 ggcttgcggg cacggtcctc tagatgatac ggttgctctc gaagcccaag ctcgggcacc
      301 atgctcaaca gaaatgaacc tcatcgcatc tgagatacag gatttttaat ggttacagag
      361 tctgattcca ccgagtcccg gatcctgcat gttcttgcca gtgaaggact ctggcttgtt
      421 acggcggagt catgcacagg aggcctgctc gcccatcgca ttgtcagcgt ggcgggagcg
      481 tcagaatgct tccttggcgg ggttgtggcc tattccaacg ccctgaagac ggacctgctg
      541 ggcgtgtcac cgacgaccct gtccactgcg ggtgccgtca gcccggaggt tgcccgcgag
      601 atggcggaag gcgcccgtga ccgctttcac gccgatcttt ccgttgcggt aacgggagtc
      661 gccggccccg gaggcggctc tgtagagaag ccggtgggct tggttttcat cgccgttgcc
      721 tggaatggag gatgtgacgt cgcccggtac gaattccagg gtgcacgcca cgccgtgaga
      781 cagcaggcgg ccgacgcggc ccttgccatg ttatgggagc gcatccagtg agcggcggat
      841 tttgccatct gcatgtccat accgaataca gcgtcctgga tggtgcgatc aaggtctccg
      901 agcttctcga tcgctgccag gattacggga tgcgcgcctg cgccatttcc gaccacggag
      961 cgatgtttgg cgtggtcgat ttctatcaga ccgccaaatc caaaggcatc aaaccgatta
     1021 tcggatctga actctacatt gcgcccgggc accgcaccga ccgctcctcc aagacgcaat
     1081 cggcggcgta caaccatttg cttctgctgt gcaaaaacga gacgggctac cgaaacctct
     1141 gcaaattgag ctccatcggc tatctggaag gatttcacta caagccacga atcgattttg
     1201 agtcgctgtc acgccatgcc gaaggactca ttgcttcgac cggatgcctg cgcggtgttg
     1261 taccccagta tctcctgcgc gaagaattcg acgaggcaga ggaagcgctc cagcgttacc
     1321 tggcgctttt cggggcagag aattttttcg ttgaactcca ggaccatggt ctgcccgagc
     1381 agcgccgcgt gaatccacgc ctggtagaca tggcgcgccg aagcggcctg atgcttgccg
     1441 caaccaacga ctgccattac ttgaacaaag aggatgccga agcgcacgac gccctcttgt
     1501 gcgtccagac caacaggacc ctcgaggaag aaaatcgttt ccggttccag accaaggagt
     1561 ttcatttcaa gagcccggag gaaatgcggg agacgttcgc cgcctggccc gaagcgatta
     1621 cgaataccga aaagatcgcc gacctctgcg agctcgaaat ccccctccac aaacatctga
     1681 tcccgaaata ttcgcccccg cccgggaaat ccaaacccga actgctccgg gaactggtca
     1741 tggagggtct ccgggagcga taccacggca agcccgctcc gcagtatgtg aaacgcgccg
     1801 aatacgagct gggtgtcatc gagcagatgg gattcgtcga ctatttcctg gtcgtttggg
     1861 acctcatcaa gaccgcgcgg gataaaggca ttcccgtagg gccgggccgc ggctcaggag
     1921 cgggcagcct ggtagcctat gccctgacga tcaccaacat cgatccgcta cgctacaacc
     1981 ttctgttcga gcggtttttg aatcctgacc gcgtatccat gccggatttc gacctggatt
     2041 tctgttatac gcgccgtccc gagctgattg aatacgtccg cgaaaaatac gggcagaaga
     2101 atgtcagcca gatcatcacc ttcggacgca tgctggcgaa acaggtgatc cgcaatgtcg
     2161 ggcgcgtgat gggcatgaac tatggcgacg tcgaccgcat cgcgaaactg gtgcccgacg
     2221 agctgaagat cacgctcaaa gacgctctgg aaaaggagcc tgagctcaaa agacaagtaa
     2281 aggaagaccc ccaggtcgcc cggttatgga gcctggcaac ccggctcgaa ggcaccatcg
     2341 gcacactggg cacccacgcc gccggcgtca tcatctgtga cgagccgctc acggaacacg
     2401 ttccgctgtt tcaggcatcc gggagcgatg ttgtcgcgac ccagttcgag atgaaaaccg
     2461 cggaacaaat cggcctgctg aagatggatt tcctggggtt gcgcactctg accgtcgttc
     2521 atgatgcggt ccggtttatt gaagagaacc gtggcgtcac gatcgatatc gactcgctgg
     2581 agccggacga ctcgctgacc tacgaattac tgcgttccgg gcgcaccacg ggcatatttc
     2641 agctcgaaag ctcgggaatg cgcgaccttt cccgccggat cgggcttgaa agcctcgagg
     2701 aagtctgcgc cctggtcgct ctgtaccggc cgggccccat gcagctcaag gaccagtaca
     2761 tcgagaacaa gcacaatcca gccaagctca gctacgacca cccgttgctc gagcctatcc
     2821 tcaaagagac gtacggcgtc gcgctgtatc aagaacaggt gatgcagatc gtgcaggcgc
     2881 tcgcaggctt ttccctgggc caggcggacg aactccgccg cgcgatggcg aagaaaaaag
     2941 ccgatctcat ggaacagcag ggcgtgcaat tcctgaaagg cgccaaagag aatggcatcg
     3001 atacagcgac ggccacttcg ctgttccaga agatcgagca gttcgccgga tacggattca
     3061 acaaatccca cagcatggcc tatgcctttg tggcgtatca gactgctttc ctcaaagcca
     3121 actatccggt ggagttcatg gccgcgctgc tcaccagcga atcaggcaac ctcgacaaag
     3181 tcgcgcaata tattgaagag tgccgccgga tggggatcga aatcctcccg cccgacgtca
     3241 accacagctg ctatggattc aatgtcgaag gcagcgccat ccgtttcggt atgggagcgg
     3301 tcaaaaacgt gggccgcgat ccggtcgagg ccatccaggc cgaacgcgaa gccaacgggc
     3361 cctacgcgga catcttcgat ttctgcgcgc gcctcgattc gcgggtgctc aaccgccgcg
     3421 ttctggaaag cctcaaccgg gccggcgcgt tcgaaagtac gggctggaac cgcaggcagg
     3481 tcgaggaatc gctggaatcc gctctgagcg aaggccagat caatcagcgg gagcgagccg
     3541 caggccagac ctcgctgctg gaactgatgg ccggcgcgag cgatgaaccg ctcatccata
     3601 aacggcctga cgtcgaggaa tggccggaga accagttgct cgcgcaggaa aaagaagtgc
     3661 tgggcttata tgtgagcagc cacccgctgg cgcggcatgc aacgctcctc gaacgcttct
     3721 cgaccatcac cctcgcagat ctcccgtcgc tggctgaagg ccacgagatc cgcatcggcg
     3781 ggcttgtctc caacgccaaa atccatgtca ctcaacgcaa ccgcaaaatg gcgtttgtca
     3841 cgctcgaaac cctcgaaagt ccctgtgaag tggtcgtgtt tcccgaattg tatgaacaga
     3901 aagccgaact gctcgtgccc gacatggtcg tcatgctgcg cgccaaagtc agctacagaa
     3961 atgacgaggt cagcctcctc gctgaggata ttgtgcccat cgatgacacc gagaagcgct
     4021 ttacggaagc cgtgcacatc cgattgagca cggtggggct tgatgaggcc ctgcttaccc
     4081 ggctcgccga gcttctggat gcgcaatctg gcagttgcga tgtcttcctg cactgcgtta
     4141 cccccgaacg tgatgagatc atcgtgcatg caaccccgtc ctgcatggtg gctccgtccg
     4201 agcgtctctg ccgcgagctt gaagaactgc tgggggaagg ttgcctgtgg tattccggag
     4261 caaacggatt cccccggcat gcgcgctgat acgtcgcgtc tctcacccgg ggcgaacgcc
     4321 ctggccgcgc aggctggatc cggcacgggc cttgccatga gcgcgcagct tcatgcatac
     4381 tggcatgtag acggtcgctg aatgcctgtt cagaccgcag acgacagaaa tgcagcgggg
     4441 agagtctccg gcacggcccc ggaacgaagg gccagcgatg gagttccgcc atgtccgtga
     4501 tcacaccacc gaaatggaag attggggagt cgtaatgcag ttagaaacag tgcgcttcgg
     4561 gacgcttgag gtggctccgg acgccataat cacctttacg caacccattc tgggtttcca
     4621 ggaataccgc cgtttcgtgc agttgcccgg gcccagtgac tatctccgct ggctccaatc
     4681 gacggaatcg agcgagctcg cgtttatcat gatggtgccc acggtgcttg tcccggatta
     4741 ccatgtagat gtccgcccgg cggagctcag cgaactggcg gtgagctcca ttgaggagct
     4801 ggaagtctac acgctggtgg ttgtgcccga ggacaaaagc aaggtgcgca ccaatctccg
     4861 ggcgcccatc ctgatcaacc ccaggctgcg gctcgccaaa caggcgctcc tggaccgcag
     4921 cgactatccc atccagttct tccttgcacc accccatcag ggccagggac aggcccggga
     4981 ggcttcccat gctggtctta acccgtaaag aggatgaaag catcatgatc ggggacgaca
     5041 ttgaggtcaa agtcctcgat ctgaaggaaa gccaggtgaa gctgggaatt gtggccccga
     5101 aatccgtggc cgtacaccgg cgtgaagtgt atctggccat tcagagcgaa aatacgcagg
     5161 cggctgcagc ggcacaaatc gacgccctta cggatttgat tcagcggtaa cgcatcgggg
     5221 aaccacagtc atggaactca tcgtagacgg caacatccga tccctggggg aagagacgcc
     5281 agccagcgtc ttcgatctgg ttgtctcggt cggccggcag ctccaggagg aaggccgctc
     5341 catcatgacg gtccagattg acggacagga gatcagcgca gggctcctgg tggagaccat
     5401 gatgaacaag ccggcggcca gcgcccggca gatcgtgatc acgtcggcag atacccgggc
     5461 gctggtgaat gagtcgctgt ccgacatgca aacctcgctg cccgatctgc cgcttgcctg
     5521 ccaggaactc gcccgcgtgt ttcagagcga gacgccggat gcggggtatg agccgtttca
     5581 acagctggca tccttgtgga gcaccatcaa ggaacgagag ctcatggtcg ccaatgccct
     5641 cgagatctcc ctggaagagc ttcagatcga tgggctctcg gtaaaacagt ttcatgacga
     5701 actcaatgcc tttttgcggg aagccactga agccctgaaa aaacgggatt cgatcctgct
     5761 ggcggacttg ctggagtacg agctggcgcc ccgggccgaa aaagaaagtg cgatcgtagc
     5821 gattctacag gaaaaggccg aacaactcgc ggggtaggaa tggccgcacc gcgcatcctg
     5881 acattcaatt ttcacgagcc gtacctgtgc ctgatggcac ggaccggcat gccctttgtc
     5941 atcggcgaat acgataaacc gccgctcgcc cggaaatggc aggaacacta tcgccccatc
     6001 cccgcaggct tcacatttct ggatgaaagc gcctggcggc aggagctcgc tgaaggccgg
     6061 tttgacctcg taatcgcgca aaatgaaatg aatgccgcca ccctgtccaa agaggtgctg
     6121 ctgtctgcaa cgcctcttct ggtgctctgc cacaaccggc gcacgttcct ggagacgacc
     6181 gcccgggtca accccgacca gccccacaac acgtttcagc gcctgctgga aaccctgcgt
     6241 gaattcgccc ggtttgtgtt tatttccgag agcaaacggg acgactatgg cttccaaggg
     6301 cccgtcatcc gcccgggtat cgacgtcgag gaatacggcg gctacacggg agagcgcctc
     6361 ggggtgctac gcgtcggcaa cgcgatgcgc ccgcgggacg ccatgttcga tgtcgatttt
     6421 caggagcagg tctgtgccgg cttccccaat cacgttgcag gcgacaaccc agacatcccg
     6481 ggatcgcagc ccgctccgac gtttgaacac ctccgggagc tttaccgcac gttccggtgc
     6541 tatctgcatg tcacccggga agccttcgag gacggctaca acctcgcgct gctcgaggca
     6601 atggccaccg gcatgcccgt ggtctccctc gcgaacccga catcccccct taccgacgga
     6661 aaagacggct ttctgtcgta tgatccggat gttctccggg gacatattga gacttttctc
     6721 aatgacctgg actatgcacg ggagatcggc gctcgcgcac gggagactgt ggcgaaggca
     6781 ttccccatgg cgcgcttcgt cgaggcctgg cgcgaaacca tcctgtcggc ggccgaattc
     6841 agtcctcgca agagcgcccg gagcccaagg cccgcgccgc cggccccctc tcccactcgc
     6901 cctgcgcccg cggcgaatat cctcctgcat tacatggcgt cgcccatcac aacgggccgg
     6961 tattttgagg ccgccctgcg caaacaccat cacgttgtca cagcgggact ccggtgcccc
     7021 gaggcaatcc tcgaacgctg ggggttttca ggcgaggcgc cgccatacgc ctcgcacgac
     7081 attgaactgg gcctggaggg gacgtgcagc gaactcctgg cacagttgcc cgaaggattt
     7141 gagccttccc tgtatctctg ggtggattcc ggcccgaaag aggcgccacc ggacctccac
     7201 agcctgcgga tccccaaagc ctgctacctc attgatacgc acctggctcc ggaactgcgc
     7261 atggccatcg cgcggcagtt cgacatcgcg tttctggcgc agaaggccca aatcccaatg
     7321 tttcaggagg ccggtatcgc gagcgtgcgc tggcttccgc ttgcctgctc gcctgaactg
     7381 cacgacgtgg ggcagctcga gcgcatctat gacgtcgcct atataggccg cattcaggaa
     7441 gatccccacg atcgccgggc cagcattctt gattgcgtgc gcagccggtt ccccaaccac
     7501 tgcatcggcc agcaatggcc ggaggatatg gcccgaacct acgcccagtc gaaaatcgtt
     7561 gtgaatgcgg ccgtcaacca cgatctcaat atgcgggtgt tcgaggcaat ggcatccggc
     7621 gcgctccttg tcaccgacca ggcggacggc ctgtcagacc tcttccagga aggtgaacat
     7681 ttcgtgcttt accgcgatga ggtggagctg tgtgcgctca ttgaacgcta cctcaatgat
     7741 cccgatgcgc gagagcgcat cgcgaaacaa ggacaagagc ttgtgctggc gcagcacacc
     7801 tacgaccatc gcatggaaca gttgctagag atggtatccg aggccacgcg catgcccggc
     7861 ggcaatgaag gagaatcccg ctaccattat ggcggctact attgttcccc acgctccgag
     7921 ttggccgccc atatcccccc ggacacgaaa cgggtcctgg atatcggatg cggagcaggc
     7981 gcgtttggac actcgctgaa acagcgcggc gtcgacgaag tcatcggtat tgaaattgac
     8041 gagcgagcca gcgccctggc cgaggaggta ttggacgggg ttttgaccgg cgatatcgag
     8101 cggatggaat taccctttga atccgcgtac ttcgactgca tcacgctcgg cgatgtgctc
     8161 gaacacctca tcgagccggc agccgctctg cgccggctgg ccccattcct cgccccggag
     8221 ggcgtcgtgg tcgccagcat ccccaacgcg cgtttctgcc aggtggtgcg gatgctcgcc
     8281 gaaggccggt ggaaatacga agacgccggg atccttgacc ggacgcatct gcgcttcttt
     8341 accgcggtag aaatgcagct gcttttccag ggagccggct atgaaatcct gaaaatgcag
     8401 ccgctttcaa tgcttccagc ggagtccctc ccgcgcaacg acgatggagg gttcacgatg
     8461 ggaagactga ccatcggccc cctggatgac gtggaatacc aggactttct tgttttccag
     8521 tatcttgtgg ttgcgaaaag agcgccgggc tcgccgctgg agcgtgcacg ggctgccctg
     8581 accgcggacc gctttgaaga ggcatgcacg atcgccgcct tggccggtga cgcggacccc
     8641 accgagcgca attttatcct cgggcaggcc ctgggccgga tgggaaggtt cgcggccgcc
     8701 gaagcagccc tccgtgaagc cctggcctcc agcccggatc gtgcagacat caccgggcaa
     8761 ctgggggtgc tgctggtcgc gatgaatcgg cacgaagagg ccatcccttg ccttgcgcag
     8821 gcgaccgccg ctgatccgaa tgactttcgt gcgctcggcg cctggggcct ggccctgctc
     8881 gcggccggcc gcctggaaga agcgtttgaa aagctcaaag cctccctgga cgttcacttc
     8941 gacaacatct cgctcatgga accctttgtc agcctcggcg aatcgcagga gcgctgggag
     9001 gatctcgaac cggtgcttcg gcgtttcgtc gaattctacc ccggaaactc cgcaatggcc
     9061 ttgcgcctcg cgcgtatcct ggtcctgttg ggaaaaaccg gcgaagcgcg ggagcatctg
     9121 gagacggtgc tcctctttga tgccggcaat gcggacgcga gccgtttgct ggaagaaatc
     9181 gcgcgtgggg gtgcatagca tgggtgaaaa tggtgaggag tgcttcgtga aaccacggat
     9241 atcacaacga tgatgaagcc ggcgcgagaa tgggcggcgg agcttgcgga cgtggtgccg
     9301 ccccccggcg tctgcctgga agaaggccgc gagggcgaac cgaccctgcg tgtcggcaaa
     9361 gcgtacctgc acagccgcta caagccccgc gaggatgccc ggcgcctgct cgattccgcg
     9421 gatctggacc ctgcccgccc ggtgctggtt gtcggcgtgg gtttgggcta tcacatcctg
     9481 gaactctgtg agcgtggtta ccgtgttgcc gcggtcgagc ccgagcgtgc tgttgccgcc
     9541 ctggcggtgc agggtcccct gcgagacagc gaggtgctgc tgggtctggg cgagccggac
     9601 gcgatcgccg cctgtccccg ctttcagcaa tttgcgcgcg aaacgccgca ggtgttcgtg
     9661 catccaccgt ccgcgcgtct ccagccggca tttgccgaag cgatggccgc gcagatttca
     9721 cgcgctgcac tgctgggcca gcgcctgaga atcgccgtcg tcgggccgat gttcggtggc
     9781 tctctgccca tcgccgacta cctggcccgt gcatttcaaa aactgggcca ccagaccatc
     9841 tatgccgaca acagcctggc ctggaacctc tacgagacca tgaccacctc tgtgaaatcg
     9901 cgcaaggccg caagtcagct gggcgaactc ctggtgcact ttctgggaga atggtcctat
     9961 gcccgcgtga tggaatttgg ccccgacatc tgtatcgtca tggcccaggc gccggtagcc
    10021 ccgcaattcc cgctccggct ggccccggca ggcatcgtta ccgcgttctg gtacgtcgaa
    10081 aattggcgcc atttgccgta ttggaaagaa cttgcgccgc gctacgacca tttttttcat
    10141 atccagcccg gcgccttcga agacaagctt cgcgagatag gatgcctgca cccggcttgc
    10201 gtattgacgg gctgcgaccc ggacatccac cgcccggtaa cgcttgagga aacggagcgt
    10261 gacgaattct cctgcgacgt ctcctttgcc ggagcgggtt acatgaaccg caaccagctg
    10321 ttgtcggggc tcacggacta tgcgctgaaa atctggggcg tgaactggac ccatccgtcc
    10381 ctccggggag tgattcagcg cccgaatgaa cggtttacgc ccgaattgtt cgccaggatc
    10441 gtcgcgggct caaagatcaa cctgaatctc cactcgagca cgagtttccc cggcgtcgat
    10501 ccccattgcg acgcgatcaa cccccgtgtc ttcgagatcg ctgcctgcgg aggattccag
    10561 ctctgcgacg catgcaaagg cctggacgcc tgttttgatt tcgactccga actgccggtg
    10621 tacacctctc ttcgggaatt gcgacacaaa ctcgatcatt acctcgccca ccccgccgaa
    10681 cgcacacaga tcgccgcggc cgcccgcgaa cgcgcactcc gtgaccacac ctacgagaaa
    10741 cgcgcggcac agatgctc
//
LOCUS       DAOPBQ010000047        10687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6019_length_10687_cov_4.069977, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000047 DAOPBQ010000000
VERSION     DAOPBQ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6019_length_10687_cov_4.069977"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKW60_02785"
     CDS             complement(<1..375)
                     /locus_tag="PKW60_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98390.1"
                     /translation="MDIQHGRRVRRLRLELEPRHAQAGIQRQAFAGMIWSKQNYLFDV
                     NLWLKGDDPHAPPPASRQAIRNTHWRHLNSMRVLSMPDKWEYPWFAAWDLAFHTVAIA
                     LIDAEFAKDQLWYMLFEQFQHPN"
     gene            361..3075
                     /locus_tag="PKW60_02790"
     CDS             361..3075
                     /locus_tag="PKW60_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98391.1"
                     /translation="MLDIHGLTVSYRLFRGAGRRIESVRVDRATLRLDAELAARIEAL
                     RTAGSAKSAAPLALVPEKVTLRDCELQVELPSLAASLTGVSVDTTFESLEEFVVEVEG
                     AALGGRVELPVSGYATSLDGGSLRIAASRSGACAVLEYCHVSLPGLAALDARAEARFE
                     EDAINFGAFVPDFRILSGGKKTVLLPKLDIPVTIDSLAVSDAHLFGKYELQTGRFTPR
                     SAQLAARAMNLSIGLDNAAWNLASLGLTAGGKYPQYTAELTINEALPLQFTASFEEDA
                     VAATLALADWPRREMLDLLPARYHAWLDAVPALERVSMQASTELRRPNFDASVSVTPG
                     FRGGSPFSGPLRAQASGALGESPALEGTAALPLEQGALAATFSAGPAAPLRLHATLDQ
                     VDPAPLVSLTRWGPLPEVLARPVSGTLSATMSAGGIDVETALETPPGSWGTELLAPLP
                     AAAEAVFRWELASNSLLGTRLALTAGEAFGAESRDWRLTLAPLGFEGAFKGTAAFPEL
                     SPVASELGAGAEIRYRAPVALHDGMLTVALDAESDYLQWGDFSFYEAPFHLTGNARLT
                     LDEGAGTLSGLALTYGDSSSLNVSEAALTLSPFHVSGPYEFESKLRLLVDAGLLESVE
                     GSIQGGGNVAWDGRLVATSDYRFEAPLLVTGGSTVALGGIAAAGNLRVDQSIAANADV
                     SAVRCALAGTPMTNITAPLTMKDTVIRLDPLAGEVFGGAFTGVASVDLSGESPVVAVD
                     MRLRNVDLARFSEKMIPQDYGLQGLAQGRIAAKLTPDGLQDATVKLVSSGDFSMNKAL
                     LQNILLEYLRDIPGAQSIERISTGVLGEAAWRSFDSASLDLGWTGNKLSGTASLRSSN
                     LNLTIDVNVDEGSIRQILAFQQKARLEDIEKIRSEPVQQSNQLMQKNE"
     gene            3122..3820
                     /locus_tag="PKW60_02795"
     CDS             3122..3820
                     /locus_tag="PKW60_02795"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HPJ98392.1"
                     /translation="MKRILVGMTAAAVMVLMGCALRTEHKIDAHITVDIRHIEQQADS
                     VLDFIEGGSDSSSAVQPAQAPSESSFISPFLHAISPIRQAHAAALNEDSPRMKEIAVS
                     LKNRFPKVEALKKQGLVGENDRGYIELRPNDALEADADRKNSTQKLIHEENNDRKELY
                     AEIVRLNPDQPNLSLAVVERIYAKKRLERAKTGEIFRLPEGKDFEEFKALLKNKQIEG
                     KLEPKEWIKVTGTK"
     gene            complement(4178..5131)
                     /locus_tag="PKW60_02800"
     CDS             complement(4178..5131)
                     /locus_tag="PKW60_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HPJ98393.1"
                     /translation="MVIAIIGILAAILLPALARAREAARRATCANNLKQMGLIFKMYG
                     NESGGEKWPSMCKAFPRYDLPCDIANPAALPNWINRFPNGPSLYPEYLTDDNILICPS
                     DGDGWTRVESDGWRHPSAMTPQGGVNPCHFWEISYEYYGWALTADHVLHPGADQNQFP
                     TPDFDPGFLAAAVSLFSGFDQTGGTSNAAGSSAVQNWATLAGRGNAFEADVTYRSMPT
                     MAEKSLYRLREGIERFMVTDINNPAGSAKAQSEIPVMWDLAWTTSDMVNGWSYFNHIP
                     GGSNVLYMDGHVEFVRFPSEWPVCKAYVAMMDLLALALPPP"
     gene            complement(5037..5717)
                     /locus_tag="PKW60_02805"
                     /pseudo
     CDS             complement(5037..5717)
                     /locus_tag="PKW60_02805"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6188..7489
                     /locus_tag="PKW60_02810"
     CDS             6188..7489
                     /locus_tag="PKW60_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HPJ98394.1"
                     /translation="MIESISGVHLLKRPVSQSSAAMQEVNMFRVTWRMLFCATLVFTA
                     RAEADVLYLYNGDRISGTLAGLGEGTLRFRGAYTAEFSVPWSDVLALTTEEEVDVVLR
                     ESGAVSGRLILLDDEQGLAFDGEARQITLGDIVTIAVPGLEMVAEASPDAPAEASEAA
                     APPKWSGTVSAGATWHKGATDAFDAVTGVAVTRQWPKDTLTMKAEAAYGEVESQKNTE
                     RMLGELKWQHYWTERFYFYWLGGAEHDAGRQLDLRLRTNVGLGREFIRTERRKLSLEA
                     GAGYRREYWLEYSLIQERVAREASRRARQARLNQYLGELGGLSGLQLVEASLRYLRDA
                     KDLEFENETSSEDSISAHVSSHYEQQVFARSLFTSDVTFEPDVDDWSNYRILSDLSFL
                     TPLSEKMSLKMRLNSEYDSYTGGVDAESWDHKFMTGLEYKF"
     gene            8695..>10687
                     /locus_tag="PKW60_02815"
     CDS             8695..>10687
                     /locus_tag="PKW60_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98395.1"
                     /translation="MMWVGLDSNASLPEVYSPLHGLANFEQVWSIAERRLPGAAVNAL
                     CTWLQQQTAERLRQWVLAPRFETVDGKHATTSQMLGALLLCAGAERCRELSNEDSVWP
                     TIRRLVPQSSDLFLYNGQPSALTRDAIGDAVRALNLRHAMDIEDTQRWFITIKLQYGF
                     TYKGARNRLAEWLVNLGTPQAVQYLAHGPEGLSCAQFKALWTTLKQFRQGRIDEADAR
                     TALTANPWIKPHWIEELLREATARIETLGRGDSEVGAPRELIEEETVCPLTGIWLNWP
                     DRQPPSLNLSLDKDVVSEQVGESPELGRIDFVVDGKRKAYWTRTNNHDWNGVTEIRID
                     DAPVTDLPRTLLVQSRAGETLISWDFADSGLADDLIFFDLDHNRIVRGQMERLDPNTH
                     FALLCDTGCEITGCSVVQTFQPANARRKLLRLPVPLSENVMVHFADFVLCQPVREPSP
                     RMTEDLIALVTPGEEVFDLGDTTGLNVEGVPDEADDAQLLIHTKNYALEVTPDGWRTA
                     QPVTLTPQFCAGQRRVRVRFQIDGAWQTRAPQLRIRVSGIALDDGNHTIKAIPRGGEI
                     NRSSDAGYLRVWVPATEGLTLWAGNCKLRFPRGGKVRLADLPGDGGELCAVHGESVYS
                     FGIRCVDRGRLESVRPGLLGQPGALLFSMADPQDFGSDGA"
BASE COUNT         2197 a         3100 c         3279 g         2111 t
ORIGIN      
        1 gttcggatgc tggaactgct caaacagcat gtaccagagc tggtccttgg cgaactccgc
       61 gtcgatcagc gcgatggcca cggtgtggaa ggccaggtcc caggcggcga accaggggta
      121 ctcccatttg tcgggcatgg acagcacccg catcgagttc aggtggcgcc agtgggtgtt
      181 gcggatggcc tggcgcgacg ccggcggcgg cgcgtgcggg tcgtcgccct tgagccacag
      241 gttgacgtcg aagaggtagt tctgcttgga ccagatcatc ccggcgaagg cctggcgctg
      301 tatcccagcc tgcgcatggc gaggctccag ttccagtcgc aggcgccgga cgcgccgccc
      361 gtgctggata tccacggcct gaccgtttcc taccggctgt ttcgcggggc tgggcggcgg
      421 atcgaatcgg tacgcgtcga ccgcgccacg ctccggctgg atgcggagct ggccgcccgc
      481 atcgaggctc tgagaaccgc cggttcagcg aaaagtgccg caccgctggc gcttgtaccg
      541 gaaaaagtca ccctgcgcga ctgcgaactc caggtggagc tgccttctct ggcagccagc
      601 ctgacgggcg tctctgtgga cactacattc gaatcgctcg aggagttcgt ggtggaagta
      661 gagggcgccg cgcttggcgg ccgcgtagag cttcctgtca gtggatatgc cacgagcctg
      721 gacgggggat ccctgcgcat tgcggcctcg cgcagtggcg catgcgccgt cctcgaatac
      781 tgccatgtca gccttcccgg tctggccgcg ctcgatgcca gggctgaagc ccggttcgaa
      841 gaggatgcca tcaactttgg cgcgtttgtc cccgatttca gaatcctcag cggtggaaaa
      901 aagacggtgc tgctgccgaa actggacatt cccgtgacca tcgactcgct tgccgtgagc
      961 gatgcccacc tgttcgggaa gtatgaactc caaaccggac ggtttacgcc gaggtccgcg
     1021 caactcgccg cgcgagcaat gaatctgagc atcggcctgg ataatgccgc atggaacctc
     1081 gcttcgctcg gcctgacggc tggcgggaag tatccccagt acaccgcgga attgaccatc
     1141 aatgaggcct tgccgttaca gttcaccgcg tcgtttgaag aagacgccgt cgccgcgacg
     1201 ctcgcactgg cagactggcc gcgccgggaa atgctggacc tgcttcctgc gcggtaccat
     1261 gcctggttag atgcggtgcc tgctctcgaa cgtgtctcca tgcaggccag cactgagctg
     1321 cggcgcccca atttcgatgc ttctgtctcg gttacgccgg ggtttcgagg cggtagcccg
     1381 ttctccgggc ctcttcgagc gcaggccagc ggcgctctcg gtgaatcccc ggcgcttgag
     1441 gggacggctg ccctgccgct cgagcagggc gctctggccg ccacgttctc cgcgggaccg
     1501 gcggctcccc tgcggctgca tgcaaccctg gatcaggtcg atccggcgcc tctggtgtcg
     1561 cttacacgct ggggccccct gcctgaagtc ctggcgcgtc cggtctcagg gacattgtcc
     1621 gccacaatga gcgctggggg cattgacgtc gagactgctc tggaaacccc gcccggctcc
     1681 tggggtacgg agctgctggc gccgctacct gctgccgccg aagccgtctt tcgctgggag
     1741 ctggcgagca actcgcttct cggcacccgc ctggctctca cggccggaga ggcatttggg
     1801 gctgaaagcc gcgactggcg tttgacgctt gcgccgctcg gattcgaggg cgcattcaaa
     1861 ggaactgcgg ccttccccga gctgtcgcct gtggcatctg aactgggcgc cggcgccgaa
     1921 atacgctacc gggcgcccgt cgcgctgcat gacggcatgt tgaccgtggc cctggacgcg
     1981 gaatccgact atctccagtg gggcgatttt tcgttctacg aagcgccgtt tcatcttacc
     2041 ggcaatgcgc gcttgacgct ggatgagggc gcgggaacgc tcagcggcct ggccctgacc
     2101 tacggggaca gctccagcct gaacgtgagt gaggcggccc tgaccctctc tccctttcac
     2161 gtgagcgggc cgtacgagtt tgagtcaaaa cttcgcctgc tcgtggacgc cggtctgctc
     2221 gaatcggtcg agggcagtat ccagggcggc gggaatgtcg cctgggacgg acgcctcgtc
     2281 gccacgtcgg actaccgttt cgaggcgccg ctgctggtga cgggcggctc gaccgtggcc
     2341 ctgggcggca tcgcggccgc cggaaatctc cgggtggatc agtccatcgc cgccaatgcg
     2401 gacgtaagcg ccgtgcgatg cgccctggca gggacgccca tgacgaacat caccgctccc
     2461 ctgaccatga aagacaccgt gatccgcctg gatcccctcg ccggcgaagt gttcggaggg
     2521 gcgttcactg gtgtcgcgtc cgttgatctg tccggagaat ccccggtggt cgccgtggat
     2581 atgcggctcc ggaatgtcga tttggcccgt ttttcagaga aaatgatccc gcaggattac
     2641 ggtcttcagg gactggcgca aggccgcatc gcagcgaaac tcacgccgga cggcctgcag
     2701 gacgcgacgg tcaaactggt gtcgtcgggc gacttttcca tgaacaaggc gctgctccag
     2761 aacatcctgc ttgaatattt gcgcgatata cccggggcgc agagcatcga acgaatcagc
     2821 accggagtcc ttggggaggc cgcgtggcgg tcatttgaca gcgcgtcgct cgacctggga
     2881 tggaccggca acaagctgtc gggcacggcc agcctccgca gctcaaacct caatctcacg
     2941 atcgatgtaa atgtcgatga gggttccata cgccagatcc tcgcctttca gcaaaaggcc
     3001 cggctcgaag atattgaaaa aatccggtcg gaacccgtac aacagagtaa tcagctgatg
     3061 caaaaaaacg aatagtccgg gcggacattc gtccagtatc gcaaacacag gagagcgtcc
     3121 catgaaacgg atccttgttg gaatgaccgc ggcggccgtg atggtactga tggggtgcgc
     3181 ccttcggacc gaacacaaaa tagacgccca catcacggtc gacatccggc atatcgagca
     3241 gcaggccgac agcgtcctcg atttcatcga gggcggctcg gactcgtctt ccgcggtgca
     3301 gccggcccag gccccgagcg agagcagctt tatcagcccc ttcctgcacg ccattagccc
     3361 catccggcag gcccatgccg cggccctgaa cgaagactct ccccgcatga aagagatcgc
     3421 cgtgagtctg aaaaaccgct tccccaaagt ggaagccctg aagaagcagg gacttgtggg
     3481 ggaaaacgac cgcggctaca tcgaactgcg gcctaatgac gccctcgagg cggatgccga
     3541 ccgcaaaaac agcacccaga aactcatcca cgaagaaaac aacgaccgaa aagagctcta
     3601 cgcggaaatt gtccggctca accccgacca gccgaacctg tctctggccg tagtcgagcg
     3661 gatttacgcc aaaaaacgcc tcgaacgcgc aaaaaccggc gagatcttcc gcctccccga
     3721 aggcaaagac ttcgaagaat tcaaagcgct gctcaaaaac aagcagatcg aaggcaagct
     3781 ggagcccaaa gaatggatta aggtcaccgg gacgaagtaa cagcgcccgg gactgactct
     3841 gcagctttcc ggtgcacccc gaagcgtcgg catggggcaa cgccgttatg atctctgttt
     3901 ttgccaggca tgccaggttt cgcgcagggt cggggccttc tcatcccctc cccacgggga
     3961 ggggatgagt tgttttagaa cctcactttc agggcagccg cgtggggatg ggcggggatg
     4021 ggcggccaga ggggcctgcc cccacagggt gcgatttccc gtgcatgaca aagggcgtgc
     4081 ggtacgatgc ccgcgcgccc ttagacgttt tcagcactcc tatacggctt gcgcgagaca
     4141 ggccacggca agcccgcgcc ggaagccgcg cgtcacgtca cggcggcggc agagccagcg
     4201 ccagcaggtc catcatggcc acatacgcct tgcagaccgg ccattcgctc gggaagcgaa
     4261 cgaactcgac gtgcccatcc atgtaaagca cgttgctgcc gcccgggatg tgattgaaat
     4321 aactccagcc gttcaccatg tccgacgtcg tccaggccag atcccacata actgggattt
     4381 ccgactgcgc cttggcgctc ccggccggat tgttgatgtc ggtaaccatg aaccgctcaa
     4441 taccttcgcg gagacgatac agagattttt cggccatcgt cggcatactt ctgtaggtga
     4501 cgtccgcctc aaaggcattg ccgcgccccg ccagagtggc ccagttctgg acggcagaac
     4561 tcccggctgc attcgaggta cccccggttt gatcgaagcc gctgaacaag gaaacggcag
     4621 cggccaggaa ccctgggtcg aagtcagggg tgggaaattg gttctgatcc gctcccggat
     4681 ggagcacatg gtcggcggta agagcccagc catagtattc atacgagatt tcccagaaat
     4741 gacagggatt tacgccgccc tgcggggtca tggcagaggg atgccgccag ccgtcagatt
     4801 ccacgcgggt ccatccgtcc ccatcggacg ggcaaatcag gatattatcg tccgtcagat
     4861 attccggata cagcgacggc ccgttgggaa aacggttgat ccagttggga agggcggccg
     4921 ggttggcgat atcacacggc aaatcatagc gcgggaaagc cttgcacatg cttggccatt
     4981 tctcccctcc gctttcgttg ccgtacattt tgaagatcaa tcccatttgc ttgagattgt
     5041 tggcgcaggt cgcgcggcga gccgcctcgc gggcgcgcgc cagggccggc aggagaatag
     5101 ccgccaagat gccgattata gcaataacca ccagcaattc tatcagggta aaaccacgtt
     5161 tcttcatcgt acctatcctt ccggtttgac cgaatgacca cgccactaga gaatactcgt
     5221 tgccactgct cgcggaagaa gtcggggtag gatgattgtc ttttacacct ccttgtaaag
     5281 gccccctcat gccgaaggct tgctcgttcc tggcctgtcg cctgagaagc gccttggtcc
     5341 ggctgtctta tggggtcggc cgcattgtaa ctatgcgtcg acgtttcgtc aataaacggg
     5401 cgcagcgaaa cagcgtctct agtgatatag cggtaggagt ccggcaggaa agggcggccg
     5461 gtattgctaa ctcgcccgcc gtaaccgggg taggcaatgt cgctgggcgc gccaagtggt
     5521 ttcgtggttc tatcgccata tttccgtacc agtacggaga ccacgacgtt gttggaggtg
     5581 ccaattacga gggtccagtt gtttttccca taagtcaacg gcggcgagac ctcaaacacg
     5641 ttccctttgc cgcccgtcaa gagcaggtgc tggaagtttt cctggtggaa cgtagcttcc
     5701 acctgatcgg caggcatgcc cagctggatg aggttacgaa agcgccggag ttccttggtg
     5761 taggcacggc cgctggtagt ccgtttaatc atgctttcgt ggcttgtcag atcgaccgtg
     5821 atcaccagaa caagtgcaca tgccacaacc ccgagcagga tgaagaatac gttcttgtct
     5881 tcaaacatga tgaacccctc ttcgcacgct tcaacgctgc acgccaaata gtaccttgaa
     5941 cttcccgggc tgtcaaggca tgtacgatct ccgggccatc ggagagtgcc tgctagggct
     6001 cctgccgggc cggcgcgcct cgaaagcccg ttttggagaa tttatgtgat gccctgcttc
     6061 tttctgtctt ctccggggat taacgaggga aaaacacgcg gccgatcgcg taacgaatag
     6121 aggctccggg caggcatgtg cccggttcat ccgcccagga ttttgcaaac cattgggaga
     6181 gtccggcatg atcgaatcca tttcgggtgt acacctgctc aaacgcccgg tttcacaatc
     6241 atcggctgct atgcaagagg tcaatatgtt tcgcgtcaca tggcggatgc ttttctgcgc
     6301 tacgctcgta ttcacggcaa gggcagaggc ggacgtactg tatctgtaca acggcgatcg
     6361 catcagtgga acgcttgctg gtctcgggga aggcacactg cggtttcggg gcgcgtacac
     6421 ggccgaattc tccgtgccct ggtccgacgt gcttgcgctg accaccgagg aagaggtcga
     6481 tgtcgtgctt cgtgaaagcg gcgcggtgtc gggccgcttg attttgctcg acgacgaaca
     6541 gggcctggcg ttcgatggcg aggccagaca gatcacgctc ggcgacatcg tcaccatcgc
     6601 ggttccgggg ctggagatgg tcgcggaggc atcgccagac gcgcccgcag aggcgagcga
     6661 agccgcggcg ccgccgaaat ggtcaggaac ggtctcggcg ggcgccacgt ggcacaaagg
     6721 ggcaaccgac gctttcgatg cggttacggg ggttgctgtg acccggcaat ggcccaagga
     6781 cactctgacg atgaaggccg aggcggccta cggggaggtc gagtcccaaa agaatacgga
     6841 gcgcatgctg ggcgagctga aatggcagca ttactggacg gagcggtttt acttttactg
     6901 gctgggcggc gcggagcacg atgccggccg gcaactggac ctgcgattgc ggaccaatgt
     6961 cggtctgggc cgggaattca tccgcacgga gcggcgcaaa ctctcactgg aagccggcgc
     7021 gggctaccgc cgggagtact ggctcgaata cagccttatc caggaacggg tggccaggga
     7081 agccagccgg cgcgcgcgcc aggcccggct caatcaatac ctgggcgaac tcggcgggct
     7141 cagcggcctg caactcgtcg aagccagtct gcgctacctt cgcgatgcaa aagatctgga
     7201 gtttgaaaac gagactagct ccgaagacag catcagcgca cacgtatcga gccattatga
     7261 acaacaggtg tttgcccgga gcctgttcac atcggatgtg accttcgaac cggatgttga
     7321 cgactggtcg aattaccgca tcctgtcgga tctgagcttc ctcaccccgc tttcagaaaa
     7381 aatgagcctg aaaatgaggt tgaattccga atacgacagt tatacgggcg gagtggatgc
     7441 cgaaagctgg gatcataagt ttatgaccgg cctggaatac aaattctagg ccgccgcgtc
     7501 aaaaacacac gcccgagcct cgcttttttt cgatttggca ctgaacagcc cgccgggaaa
     7561 agggcggggg tagaaaagaa gaagaaggga tggcggagag gccgggatcc gaagtggcgc
     7621 ctttcataac tcgctgccag ataatgcctt gctgaagaaa gccttttggc ttcaagtggt
     7681 tatatttcac tctttgtcat ccgacatcat tgcaggtcac tcctgagcat cgtgatttgc
     7741 cacagatttt gcatcaagtt tgcacaaatc ggaattcaaa gatccctcta ggtggcctcg
     7801 agaaaaaggt actccgccaa acaagctgct ttgcccgagc cttggatcta tcccgcttcc
     7861 tctggcgcca accatccctt ggccgctgga ggactgcgta atgcgtacag ggggttccag
     7921 aatgcaggtt gggtcggtta ccatgccgta ccacgcctgt gagtttgcct gtggctgcgg
     7981 aaggatcttc tatggaacgg ctcttcctcc ctgtcctgtc tatggtcatg caatggaacc
     8041 gacctcgata caatgctcag ttccaattcc tcggagcaca gatcgaattc tccgctcccg
     8101 agtcgacgcc agcatatggc tcgaggatat cggtgtaacg ccgcgcttcg ttgtccatga
     8161 gcacgacaca aaactcagca agaagttccg tgaattctgg aaggagcaga ccggtgcgag
     8221 gcgcatccga ataccattca aggccccaaa ggccaacgcc atgaccgaga cctggagcga
     8281 aggatgcaag cgcgagtgct tggatttctt catgtgctgc ggctttgatc ggctcgacta
     8341 tatcaaaacg acctgggccc gttattgcaa tacgaggcgc ccacaccgag gcttcggcat
     8401 gaacaacgta gtcctcgaca agacgttcca acctgaaacc gagggcgcca tttgctgcag
     8461 acaagagctc ggtggtagca ttgagtcctg ccacaaagac acagcgtgaa tactggcgcg
     8521 acgatggcca gtgtgtgcaa agagagagtt ggtgtgaaaa atggggtcat gttgtggaag
     8581 ctgtttctga gcacgcatgg ggtaatactc acaatgctcc gcagtgacca cggagtaagc
     8641 tgaggcaatt ccacgctggg acaccgtccc aggctcaagc cgcctggagg tagaatgatg
     8701 tgggtggggc ttgattcaaa tgcctcattg ccggaggtat acagtccgct tcatggcctg
     8761 gcgaacttcg agcaagtgtg gtctatcgcc gagcggcgcc tgcctggtgc agctgtcaac
     8821 gcgctttgca cgtggcttca acagcaaaca gccgaacgtt tgcgtcagtg ggtactcgca
     8881 ccgcgctttg agacggttga cggcaagcat gcaactacga gccaaatgct cggcgcgcta
     8941 ctgctatgtg ccggagcgga acgttgtcgt gagctgagta acgaagattc tgtttggccg
     9001 acgatccgca ggttagttcc gcagtcatcc gacctcttcc tgtacaacgg gcagccgtca
     9061 gctctgacgc gggacgccat tggcgatgcg gtgcgcgcac tgaatctccg ccacgcgatg
     9121 gacattgagg acacgcagcg ctggttcatt accataaaac tgcaatatgg cttcacgtac
     9181 aagggagcgc ggaatcggct tgcggagtgg ttggtcaacc tgggaacgcc gcaggcggtc
     9241 cagtatctcg cccacggccc agaggggctt tcttgcgctc aattcaaggc tctgtggacc
     9301 actttgaaac agttccggca gggccgtatt gatgaggcag acgcgcgcac cgcgctcaca
     9361 gcaaatccct ggataaaacc ccactggatc gaggaattgc ttcgagaggc gacggcaaga
     9421 atcgagacgt tagggcgcgg cgacagcgaa gtaggtgctc ctcgcgagct gattgaggag
     9481 gaaaccgtat gtcctctcac cggaatttgg ctgaactggc cggaccgaca acctccaagt
     9541 ttgaatcttt cgcttgacaa ggacgtagtg tcggagcaag tcggcgagtc ccctgagctt
     9601 ggccgcattg actttgtggt tgacggcaag cgcaaggctt actggacacg aacaaacaac
     9661 catgactgga atggtgtcac tgagatcagg atcgacgatg cacctgtaac agatctgcca
     9721 agaaccttgc ttgtgcaatc acgtgcgggc gaaactctca tttcgtggga ttttgcagac
     9781 tccggactgg cggacgacct catctttttt gatctcgacc acaaccgaat cgtgcgcggc
     9841 cagatggagc ggttggatcc aaacactcat tttgcacttt tgtgcgacac tggctgtgaa
     9901 atcacgggat gttccgttgt gcaaacgttt cagccggcga atgcaagaag aaagctgctg
     9961 cgtttaccgg ttccactctc tgagaacgtg atggtgcatt ttgcggattt tgtgctgtgc
    10021 cagccggtgc gcgagccgag cccacggatg accgaagacc ttattgccct tgtaacgccc
    10081 ggcgaagagg tttttgattt gggtgataca actggactga atgtcgaggg agtgcccgat
    10141 gaggcggatg atgcccagtt gctgattcac actaagaact acgcgcttga ggtaacgccg
    10201 gacggttggc gtacggcgca gccggtgacc ctgacgccac aattctgcgc cggtcaacgg
    10261 cgggtgcgcg tgcgctttca gattgatggc gcctggcaaa cacgtgcccc acaactccgg
    10321 atacgtgtct cgggcattgc gctggacgac ggaaatcaca ccatcaaggc gataccgcgt
    10381 ggcggcgaaa ttaaccgatc cagtgatgca gggtatctgc gggtgtgggt gccagcaacg
    10441 gaaggcttaa ctctgtgggc aggtaactgc aagctgcgat tccctcgggg gggcaaggta
    10501 cgattggccg acctgccagg ggatgggggc gaattgtgcg ccgtccacgg cgagagcgtg
    10561 tattcgtttg gcattcgctg cgttgatcgg gggcgattgg aaagcgtgcg gccgggactt
    10621 ctcgggcaac caggggcact gctgttctca atggcagacc cccaggactt tggaagcgac
    10681 ggtgctg
//
LOCUS       DAOPBQ010000048        10684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6022_length_10684_cov_4.990874, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000048 DAOPBQ010000000
VERSION     DAOPBQ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10684
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6022_length_10684_cov_4.990874"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PKW60_02820"
     CDS             <1..1033
                     /locus_tag="PKW60_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPJ98396.1"
                     /translation="IDGVLHDVMSIDHEIEAAVVSRVKIMADMDITETRRPQDGHITF
                     DFADNEFDIRVATMPCFLGERVVLRLLDASTVLQGIKDLGLETEDVKKLSALIEQPYG
                     MILVTGPTGSGKTTTLYAALNQKNVLTESIVTLEDPVEYQLSGINQVQIDPDIDLSFA
                     NTLRAAMRQDIDVLLVGEIRDAETARIAIRAAMTGHLVFSTLHTNDAPEAISTLRNMD
                     VPSYLLASSLTAVVAQRLVRKICPKCRSAFTPTKAMLKVLGLPESTKKLYRGEGCNFC
                     YHTGAKGRTGIFELLEVNSSIRQMIAAAEPTEKIARAAKLKTMADRCRIKVKEGIIAP
                     EEFLRVIRT"
     gene            1786..2892
                     /gene="dnaN"
                     /locus_tag="PKW60_02825"
     CDS             1786..2892
                     /gene="dnaN"
                     /locus_tag="PKW60_02825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPJ98397.1"
                     /translation="MKITIGRQDLLDAVNKVKAVVSPKSALPILSHILMETGESQVRL
                     TATDLKISIECSVDCTVEEPGALTVSSQRLSSIVSELPVSDVHLTLAESNVVMLKCGK
                     IETKLFSMSPDEFPPMRSFDHVEPIILQQSMLKSLFSKTSFAICSDQTRYNLTGLLCE
                     IQDGRITVVATDGRRMSLCMETENIPQGLSVKVIIPGKMIAELERLLSGDGEVRLFFD
                     ETQAAFVFDSNRLVSALIEGTFPNYDMVIPKKHDKEAYMNTGAFVEAIRRTRTMTNEK
                     FNSVRFMFDQNQLTLRVVTPEVGEYQEEMEAEFSGDPMEIAFNPDFILDALRHIDTPK
                     TCLVLKDGMSPGLIKPFTEAPVDNYLNIVMPIRI"
     gene            3664..4617
                     /locus_tag="PKW60_02830"
     CDS             3664..4617
                     /locus_tag="PKW60_02830"
                     /inference="COORDINATES: protein motif:HMM:NF025841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HPJ98398.1"
                     /translation="MGMRDGGARLSGTLWWLTPDDLALSASAWEEELDHLQALGIDTI
                     IFNGPFAGPEGGAAFDALLERLDVRGLHVYLDMLSAPDWWRLEGAGEEVRRASDRATA
                     LQQAYGHHASFTGFYIPYECYVMWGRQGALVRDLYAGVSDGCKMAAPEKPVMISPFFR
                     LDKACYLGDFQWATPEEYAVFWRDILSAASIDIVALQDSGEHLSSYTLAQRRPFFEAM
                     AQACSATGTSLWANVESGELHVASLEDYAARFGLKTPVNSPETEPFWRAVPAEKFRAK
                     LSMAGEYTDTAITWGYREFLRPSRGLEAAQAYDGYVRVFAR"
     gene            4774..6639
                     /locus_tag="PKW60_02835"
     CDS             4774..6639
                     /locus_tag="PKW60_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPJ98399.1"
                     /translation="MTPDRNHEQPNRVFSCSVGDMEASRAFSQIFDLGAAEKNASQER
                     GCIAVAEENASGDAAFSAQLQTLLEIHNELSITPGLDELCETAVALAVARLGFERLSI
                     WFCDAEPGVLRGSFGVDENGALRDERACRVQLTDDEDMRRVLNREVKFVLRSPVNLRD
                     AEGKTVGQGTHVMAPLWDGEDVVGIICADNRLTHREITEDQCRLLALLAVLVGHLCSL
                     HRRSAAIRHGEEEYRQLEAEMQHAQKLESLGVLAGGIAHDFNNLLVAMLGNVELAQVE
                     LDSGSPVQRYLRDIATAAARGADLCRQMLAYSGKGRLVQELVDLNDLAREMAGLIGVA
                     ISKNTQVEFRLTDMLPFVEGDATQLRQVVMNLVTNASEALGDQPGVVTIETGVVDWAR
                     IDTEVIQAGENMRPGEYVYLEVADTGCGMDQSTLEHIFDPFYSTKFADRGLGLAAVLG
                     IVRRHHGGLRVDSAPGKGSAFRVLLPPKLAAVPQSAAGEMRVEAAGSGKVLIIDDEPQ
                     VRQLGAAMLGMAGYQALLAPDGESGLALFEKHAGEITVVLLDMAMPGMDGRETFERIR
                     GIDPAVPVVLCSGYGPDGSAEQFPDGELAGFLAKPFRMSELLAAVKAVVGAQGTD"
     gene            6823..6960
                     /locus_tag="PKW60_02840"
     CDS             6823..6960
                     /locus_tag="PKW60_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HPJ98400.1"
                     /translation="MTWLKLTAATLVFVFGVFSFMGCNTIRGMGKDIQKGGQALEEAA
                     R"
     gene            7141..9468
                     /locus_tag="PKW60_02845"
     CDS             7141..9468
                     /locus_tag="PKW60_02845"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPJ98401.1"
                     /translation="MRTYHRWAAMLCAALAVALMCGGCARLGSLPKGGALTLAERGQT
                     GFTIVIAEDASPSTRYGAEELQHYLHEITGAEFPVQTDAVPMGPHEIILGDSRHLRQL
                     GMQVDFDALGDEGYVIRTAGPHLVIAGGALRGNLYGVYGLLDDHLGCKWFTAQVERIP
                     KKTTLRLAVLDDTQIPVLEYREPFVIDCFDGDWCARNRVNSSAASLEDRHGGKVRFGA
                     GMFVHTFDVLVPPKIWFDEHPEYFSEIGGKRLKERTQLCCTNPEVVRLVTEELKKRIA
                     ADPDAFVYSVSQNDWDNYCECAACQALAEKEGSQMAPVLQLVNQVAEAIEQDYPDKAI
                     ETLAYQWTRKAPKTIRPRPNVIIRLCSIECCFMHPLATCDSEANRAFCRDAEEWARVA
                     NRLWVWDYVTSFHHFHVPYPNLRVRDDNIRFFIEHNVTGIFEQDNYQSRSGEFNELSG
                     YLNAKLLWNADYGEDRAINEFLEGVYGKAAKPIREYLDMLHDKVEKDNIHCDIWIGPW
                     EAPYLTDEVMAKGEALWNRAEAAVADEPEVLERVKIARLCHDYSWLELNRYKGDVLYA
                     FDHAHFTVEPRPEFERRVQTFFEMARKGGVVRMDESATTLDDYARSFELALKGGQQTL
                     EPCAAAAVANPRPGIAFKAYTGVWNALPDTTKLTPAAEGIASHVHDDAWPQDANYALC
                     FSGYFEAPADGVYTFTIGSNDGSRLTLGDKVLDNDGLHSIREKFMHVALRKGYHPLRV
                     EYFQEGGSKALYLRYDGPGISNRLIPPEAYFHDAP"
     gene            9572..>10684
                     /locus_tag="PKW60_02850"
     CDS             9572..>10684
                     /locus_tag="PKW60_02850"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPJ98402.1"
                     /translation="MMYFGTCYYPEHWTPRQAKDHIPLMKQARINVVRMGEFAWSRFE
                     PEQGRYRFEWMDAVIQALHKEGISTVLCTPTCIPPQWAVQKHPAMLQRDADGRVRNPG
                     SRCHCCKNAPEYQILADRIVDQMGRHYADMPGVIGWQTDNEFGCHSSTRCYCDHCQSA
                     FRDWLLAKYKDTDSINQAWGTAFWGFEFQQWNEIPLPRHMPAGENPSHWLDFARFSSD
                     TQVKFQRSQYDLLKQLCPKHFVTHNFMGRFPEIDYWKLAACTDFPSWDNYPEGKEDPF
                     LPAYQHEITRSFTGRFWVMEQKSGPTGDSQTGLMGEQPEPGELRRWTWQAVANGADGV
                     CYFRWRACLSGAEQYWHGILDHDGLVRRRYREVAETG"
BASE COUNT         2257 a         2846 c         3348 g         2233 t
ORIGIN      
        1 catcgatggc gtgcttcatg acgttatgag catcgaccat gagatcgaag cggccgtggt
       61 ctcgcgggta aagattatgg ccgacatgga catcaccgag acacgccgcc cacaggacgg
      121 gcatatcacg tttgattttg ccgacaacga gtttgacatt cgcgtggcaa cgatgccgtg
      181 cttccttggc gagcgcgtgg tattgcggct gctggatgcg tcgacggtgc tccagggcat
      241 caaggatctc ggccttgaaa cagaggatgt gaaaaagctc agtgcgctga tcgaacaacc
      301 ctacggcatg atcctggtca cagggcccac cggcagcggc aagaccacga ccctctatgc
      361 ggcactcaat cagaaaaacg tgcttaccga aagcatcgtg accctcgagg atccggtgga
      421 ataccagctg tcgggtatca atcaggtgca gattgatccg gacatcgatc tcagctttgc
      481 caatacgctc cgcgccgcca tgcgccagga tatcgacgtg ctgctggtcg gtgaaatccg
      541 cgacgcggaa acggcgcgga ttgccatccg cgcggcaatg acggggcatc tcgtgttcag
      601 cacgcttcac accaatgacg caccggaagc gatcagcaca ttgcgcaaca tggatgtgcc
      661 gagctacctc cttgcgagtt ccctgacggc ggtcgttgcg cagcggctcg tgcggaaaat
      721 ctgcccgaaa tgccggtccg cattcacgcc gaccaaggcc atgctgaagg ttctcggcct
      781 gcccgagagc accaagaaac tgtatcgcgg tgaaggctgc aacttctgct accatacggg
      841 cgcgaaggga cgcaccggca tcttcgagct gctcgaagtg aattcctcga tccggcagat
      901 gattgcggca gccgagccga cagaaaagat cgcgcgcgcc gcaaaactca agaccatggc
      961 cgatcgctgc cgcatcaagg taaaggaagg cattatcgcg ccggaagagt tcctgcgcgt
     1021 tatccggacg taacaaaacg attgcgcaga ttgcaggcct cgtgccccgc gagacggcca
     1081 caacgatcgg cacggtttac ctgacctggg tcacggccgc gagtgatcca aatgtgaata
     1141 agttgtggaa aacttggcac agagcattgg cagtcccggg ttgggaaacg gcgtccacaa
     1201 ctcataagtt gtcattttaa acaagttaga acgcaaaccc cgttttttta ctcgaaattc
     1261 catttttata aggctgtgtg aataaatagc ttaggaatcc atgcgtacgt ggcgatccgg
     1321 gttcatatcc aggtccgggg tatgttccac gccacaggat gagctcaaag ccatccacga
     1381 agacggcaac aattacagga tgaatcagag ccaaacaaga atcatcctgt gtggaacaat
     1441 ttgatgaaaa gtggatacgc tacggttggg gataattttc tggataagag gggataaggc
     1501 gcctggtttt tcctcaattt ttccaccggc gccggcgagg aaatcgggaa ttgaccgcgg
     1561 ctcgcgtgat ggatgtggaa agtgtggata atccggcact gcttgtccag acagtatcaa
     1621 caagataaac tgtttgtacg caatgcgctt acatcgatgt tcacagtata cagtcctcta
     1681 tgacttcgaa tacttgaact ttaaataaaa aaaagaggat catacgatct ggaaaacaaa
     1741 tccacccact ctgtgggcaa cggggactcg gagggaggca cgggaatgaa aatcaccatc
     1801 gggcgacagg acttgctgga tgccgtcaac aaggtgaaag cggtggtgtc gccgaaatcg
     1861 gcgttgccaa tactctcgca tattctgatg gaaacgggag agagccaggt tcgcctgacc
     1921 gctacggatc tcaagatcag cattgagtgt tcggtggatt gcacggtcga ggaaccgggc
     1981 gcgttgacgg tgtcctcgca gcgtctgtcc tcgattgtgt cggagctgcc cgtcagtgat
     2041 gtgcacctca cgctggcgga gagcaatgtg gtgatgctga aatgcggcaa gattgaaacc
     2101 aagctgttca gcatgtcgcc ggatgaattt ccgcccatgc gaagttttga tcatgtggaa
     2161 ccaatcatcc tgcagcagtc gatgctcaag agtctttttt cgaagacatc gttcgcgatt
     2221 tgttcggacc agacccgcta caacctgacc ggacttctgt gcgagatcca ggatggccgg
     2281 atcaccgttg tagcgacgga tggacgccgc atgagtctgt gcatggagac cgagaacatt
     2341 ccgcaggggc tttccgtcaa agtcattatt ccggggaaga tgattgcgga gctcgaacgg
     2401 ttgttgagcg gcgatggcga ggttcggctg ttcttcgatg agacgcaggc cgcctttgtc
     2461 tttgattcaa accgcctggt gagcgcgctg attgaaggga cctttccaaa ttacgacatg
     2521 gttatcccca agaagcacga caaagaggcg tacatgaata ccggggcttt tgtggaagcc
     2581 atccgccgta cgcgcaccat gaccaatgaa aagttcaatt ccgtccgctt catgtttgat
     2641 caaaaccagc tgaccttgcg cgtcgtgact ccggaggtcg gcgaatatca ggaagaaatg
     2701 gaagccgagt tcagcgggga ccccatggag atcgctttca atcctgactt tattctggat
     2761 gcgcttcgcc acatcgacac cccgaaaacg tgtcttgttc tgaaagacgg catgagcccg
     2821 ggcctgatta agccgtttac cgaagcgcca gtcgataatt acctcaacat cgtcatgccg
     2881 attcgcatct aacgtggcgg tttcgctggc agccggttcg agagtatttc aggggcaatc
     2941 catccagatg gattgcccct cgtacattct gtgcaagatc gcgggcgtcc caggttccca
     3001 atttaaccgt tgacgccatc ttgtgacgaa acacagacga tccggcgcaa aaatgacttc
     3061 gctagacttc ttttatgccc tatattgtgg tgtggataaa gcatcctaca ctggggacag
     3121 tgcctgtgga tgagttgtgg aaatcccgtt ttatttcaaa aaacaaggtg ttttctggtc
     3181 caggataatc ctttttcttc gttatttaat tttgttctaa ctatttgatg ctaaaaagat
     3241 tatacctaag actcctaagg agatcccaag ctgtctcccc ttcaaaaccg gtcttggatg
     3301 agctcacaac acgccagtgg ataactttgg tttttggagc ttatttacct ctatatcttg
     3361 tggttggcca atgttattta ataatagcaa tatagtgttg acatttattt actgaaattg
     3421 cgtataccgg ccgcgggctt tctctgttta ttgctcgtga tattcttggt ggaaccggcc
     3481 aaaagcccgg aaatttggcg aaatcggcgc atgcggtttc ggctggcaac gtcccgggaa
     3541 gagcacgaga gggacttcaa ttaaggtatg acggagatga atcgttattg ctggattctg
     3601 ggtgcggcag gcctggtttt ctgttgtgcg ctgtcagtta tgggggcggg aggttgtgcg
     3661 tcgatgggaa tgcgagacgg aggcgcacgc ctttcgggaa cgctctggtg gttgactccg
     3721 gacgacctcg ccttgtccgc aagcgcctgg gaggaggaac tggaccacct tcaggcgctg
     3781 ggtatagaca caatcatttt caacgggcct tttgcgggtc ctgaaggcgg ggctgcattc
     3841 gatgcgctgc ttgaacgcct ggatgtgcgc gggctgcatg tgtacctcga tatgctgtcc
     3901 gcgccggatt ggtggaggct cgagggtgcc ggagaagagg ttcggcgcgc atctgaccgg
     3961 gcaacagcgc ttcaacaggc atatggtcat cacgccagtt tcacgggctt ctatattccg
     4021 tacgaatgct atgtcatgtg ggggcgccag ggagcgctgg tgcgcgacct gtatgccggg
     4081 gtgtctgacg ggtgcaagat ggcggcgccg gagaaaccgg tgatgatttc accgtttttc
     4141 cggctcgata aggcgtgtta tctcggggat ttccagtggg caaccccgga agagtatgcc
     4201 gtgttctgga gggatatttt gagcgcggcg tcgattgata tcgttgcatt gcaggacagc
     4261 ggggaacatc tttcaagtta taccctggcg caacggcgtc cctttttcga agctatggcg
     4321 caggcttgtt cggccaccgg cacttcactc tgggctaatg tggagagcgg cgagctgcac
     4381 gttgccagcc tggaggacta cgcagcccgg ttcggcctga aaacgcctgt gaacagtccg
     4441 gagaccgagc cgttctggcg cgcggtgccg gcggaaaaat tccgggcgaa gttgtcgatg
     4501 gcgggagagt acacagatac ggccatcaca tgggggtacc gggaatttct ccggccatca
     4561 cgcgggctgg aggccgctca ggcgtatgac ggctatgtac gtgtattcgc ccggtgacga
     4621 ggccgcagag gcaaattcaa ggcctctgaa actaccggcc ggtttacgat ccgcaaaaaa
     4681 aatggtataa cacaagcgtg gttcagtggg tcctggggag cattttgcgc agcacactgc
     4741 atgtaacata ccgcgacggg ggaaatacgc cgcatgacgc ctgatcgaaa tcatgaacag
     4801 ccgaatcgtg tgttttcgtg ttccgtgggc gatatggagg catcacgcgc attttcacag
     4861 atttttgatc tcggggcggc ggaaaaaaat gcgtctcagg agcgggggtg cattgccgtt
     4921 gcagaagaga atgcctccgg agatgccgca ttcagcgcac agttgcagac gcttcttgaa
     4981 atccataatg aactctccat aacgcccggc ctggatgaac tgtgtgagac agcggtagcc
     5041 cttgccgttg cgcggctggg ctttgaacgc ctcagcatct ggttttgtga tgccgagccg
     5101 ggcgttctgc ggggttcgtt cggggtggac gaaaacggcg cattgcgcga cgaacgcgcg
     5161 tgccgggtgc agctgacaga cgatgaagac atgcgccgcg tcctgaaccg tgaagttaaa
     5221 tttgtgttgc gctctcctgt caaccttcgc gatgccgaag gcaaaacggt cgggcaggga
     5281 acgcatgtga tggcgccgtt gtgggacggc gaagatgttg ttggaatcat ttgcgccgac
     5341 aaccggctga cccaccggga gatcacggaa gaccagtgcc ggctcctggc gctgttggcg
     5401 gttctggtgg ggcatctgtg ctccctgcac cgcagatcgg cggcgatacg gcacggtgag
     5461 gaagagtacc ggcagctgga agcggaaatg cagcatgccc agaagctgga gagcctgggc
     5521 gtgctggcag gcggtattgc gcacgacttt aacaacctgc tggttgcgat gctggggaat
     5581 gtcgagctgg cccaggtcga gctggattcc ggttctccgg tgcagcggta tttgcgggat
     5641 atcgcgaccg cggcagcccg cggagcggat ttgtgccggc agatgctggc ctattcgggc
     5701 aagggccggc tggtgcagga actggtggat ttgaatgatc tggcccggga aatggctggg
     5761 ctcattggtg tggccatttc aaaaaacaca caggtggaat tccgcctgac ggacatgctg
     5821 ccattcgtgg aaggagacgc cacgcaattg cggcaggtgg tgatgaacct tgtgaccaat
     5881 gccagcgaag cgctcggaga tcagccgggc gtggtgacaa ttgaaacagg cgttgtggac
     5941 tgggcccgga tcgatacgga ggtcattcag gccggggaaa acatgaggcc gggcgaatat
     6001 gtgtatctgg aggttgccga cacaggatgc ggcatggacc agagcaccct ggagcacatt
     6061 tttgatccat tctactcgac caagtttgca gaccgtggct tgggcctggc agccgtgctt
     6121 gggattgtcc gccggcatca tggagggctt cgcgtcgaca gtgctccggg gaaaggctct
     6181 gcgtttcgag tactcttgcc accgaaactc gcggcagttc ctcagtcagc tgcgggtgag
     6241 atgcgcgtgg aggcggcggg ctcgggaaag gtgttgatta ttgacgacga gccgcaggtc
     6301 cggcaactgg gggccgccat gctcggcatg gcaggttacc aggctctgct ggcacccgat
     6361 ggcgaaagcg ggctggcgct gttcgaaaag catgcgggcg agattactgt ggtgctcctg
     6421 gacatggcga tgccggggat ggacgggcgc gagacatttg agcggattcg cgggatcgat
     6481 ccggcggttc ctgttgtgtt gtgcagcggc tacggcccgg atggctccgc agagcagttc
     6541 ccggacgggg agctggccgg attcctggcg aaaccgtttc gtatgagcga actcctggcg
     6601 gccgtgaagg ccgtagttgg cgcgcagggg actgattgat tcatctcggg cgtctcggcg
     6661 gcgccggtgc gggacggatt cgatttttat ttcaaatagt gacaggtgcg tgcggccgca
     6721 ggcatttctt cccagcttca cgtgcgcgcc cggcctctgt ctggttgact cgacttcccg
     6781 cacattctat cctgtaggcg tggaagtcag gaggaatgac gtatgacctg gttgaaatta
     6841 acggcagcta cgcttgtctt tgtgtttggc gtgttcagtt tcatggggtg caacacgatt
     6901 cggggtatgg gcaaggatat ccagaagggc ggccaggcgc ttgaggaagc cgcccgctga
     6961 ccctcgactt gactcatgcg gtcccggtgt ccggccaggg cctgcgattg tcttcttgtt
     7021 gacaaaaaca ttcagtgcgc agtccggccg gcccgccggg cccgcagagg gcattcaggc
     7081 cggaggcgcc tgcgccgtac cgtgttcatt gctgcattca ctgagaggga ggattatctc
     7141 atgcggacat accatcgatg ggcggcgatg ctctgtgcag cgttggccgt tgccctgatg
     7201 tgcggcggct gtgcgcgcct ggggtcgctg ccgaaaggcg gcgcgctgac actggcggag
     7261 cgcgggcaga ccggcttcac cattgtcatc gcggaggatg catcgccgtc gacccgctat
     7321 ggcgcggaag aactgcagca ttacctgcat gaaatcacgg gtgcggaatt tccggtgcag
     7381 accgatgccg tgccgatggg gccgcacgag atcatcctgg gcgacagcag gcatttgcgc
     7441 cagctcggca tgcaggtgga ttttgacgcg ctcggcgacg agggctacgt catccggacg
     7501 gcagggccgc atctggtcat cgcgggtggg gcgttgcgcg gaaatctgta cggcgtctat
     7561 ggcctgcttg acgaccatct gggctgcaaa tggtttacgg cgcaggtcga gcgcatcccc
     7621 aaaaaaacca cgctccggct cgctgtgctg gatgacacgc agatcccggt gctggaatac
     7681 cgggagccgt tcgtgatcga ttgttttgac ggcgactggt gcgcgcgcaa ccgggtgaac
     7741 agcagcgcgg cctcgctgga agatcggcac gggggcaagg tgcgttttgg cgcgggcatg
     7801 tttgtacaca ccttcgacgt gctggtgccg cccaagatct ggtttgacga acatccggaa
     7861 tatttttcgg aaatcggcgg caaacgcctc aaagagcgca ctcagctttg ctgcaccaac
     7921 cccgaagtgg tgcgtctggt cacggaagaa ctgaaaaagc gtattgccgc ggacccggac
     7981 gccttcgtgt attcggtttc gcagaatgac tgggacaact actgtgaatg cgcggcctgc
     8041 caggcgcttg ccgaaaagga aggctcccag atggcgccgg tgctgcaact ggtcaatcag
     8101 gttgccgaag ccatcgaaca agactatccc gacaaggcca tcgagactct ggcgtaccag
     8161 tggacgcgca aggcgcccaa aaccatacgg ccgcgcccca acgtcatcat ccggctgtgc
     8221 agcattgaat gctgtttcat gcacccgctg gccacgtgcg acagcgaggc aaaccgggca
     8281 ttctgcagag acgccgagga gtgggccagg gtcgccaacc ggctctgggt ctgggattac
     8341 gtgaccagtt tccaccattt tcacgtgccc taccccaacc tgcgggtgcg tgatgacaac
     8401 atccggttct ttatcgagca caacgtcacg gggatcttcg aacaggacaa ttaccagtcg
     8461 cggagcggcg agttcaacga gttgagcggt tatctgaacg cgaagctcct gtggaatgcc
     8521 gactatggcg aagatcgcgc catcaacgag tttctggagg gcgtgtacgg caaggcggcc
     8581 aaacccatcc gcgaatatct cgatatgctg cacgacaagg tggaaaagga caatatccac
     8641 tgcgacatct ggattggccc ctgggaagcc ccgtacctga cggacgaggt gatggcgaaa
     8701 ggcgaggcgc tgtggaaccg cgccgaggcg gccgtcgcag acgaaccgga ggtgctcgaa
     8761 cgagtcaaaa tcgcccgtct ctgtcacgat tacagctggc tcgagctgaa ccgctacaag
     8821 ggcgatgtgc tgtatgcgtt tgatcacgcg catttcacgg tcgagccccg gcccgagttc
     8881 gagcggcgtg tccagacgtt ttttgaaatg gcgcgcaaag gaggggtggt ccgcatggat
     8941 gagagcgcca ctaccctcga tgactatgcc cggagtttcg agctcgcact caaaggcggc
     9001 caacagacgc tcgaaccctg cgcggcggcc gcggtcgcga acccgcgccc cggcattgcc
     9061 ttcaaggcgt atacaggtgt ctggaacgcc ctccctgata caacgaagct gacgcccgcc
     9121 gcggaaggca ttgcatcaca cgtgcacgac gacgcgtggc cgcaggacgc gaattatgcc
     9181 ctgtgttttt cggggtattt tgaggcgccg gcagacggcg tttacacgtt caccattggc
     9241 tccaacgacg gcagtcgcct gaccctgggc gacaaggtcc tcgacaacga cggcctgcat
     9301 tccatccggg aaaagttcat gcacgtggcg ctgcggaaag ggtaccatcc cctgcgcgtg
     9361 gagtatttcc aggagggcgg atccaaggcg ctgtatctgc gctacgatgg cccgggcatc
     9421 agcaaccgcc tcattccgcc ggaagcgtat ttccacgacg ccccgtgacg agaaccgggt
     9481 gcgccgggga tctgacgggt acgggcgcat tggcccggcc gggatgccgt tgctatgata
     9541 ccgcccggac acagagcgaa gggaaaccgg catgatgtac tttggcacgt gttactatcc
     9601 cgagcattgg acgccccggc aggcgaaaga ccacatcccg ctgatgaaac aggcgcggat
     9661 caacgtggtg cgcatgggcg agtttgcctg gagccggttt gaacccgaac agggacgcta
     9721 ccgcttcgaa tggatggacg cggtcattca ggcgctccac aaagaaggca tctcgacggt
     9781 cttgtgtacg cccacgtgca tcccgccgca gtgggcggta cagaaacatc cggccatgct
     9841 tcagcgggac gcggacgggc gggtgcgcaa tcccggctcc cgctgccatt gctgcaagaa
     9901 cgcgccggag tatcagatcc tggccgatcg catcgtggat cagatggggc gccattacgc
     9961 ggacatgccc ggggtcatcg gctggcagac cgacaatgag ttcgggtgcc acagctcgac
    10021 ccgctgctat tgcgaccatt gtcagagcgc gttccgcgac tggttgctcg cgaagtacaa
    10081 ggataccgac agcatcaacc aggcgtgggg caccgcgttc tgggggtttg agtttcagca
    10141 gtggaacgag attcccctgc cccggcacat gccggccggg gagaatccca gccactggct
    10201 cgatttcgcc cggttttcat cggataccca ggtgaagttt caacggtccc agtatgattt
    10261 gctgaagcag ctgtgcccga aacatttcgt cacacacaat ttcatggggc ggtttccgga
    10321 aattgactac tggaagctgg ccgcctgtac tgattttcca tcgtgggaca actatcccga
    10381 gggcaaagaa gacccctttc tgccggcgta ccagcacgaa atcacgcgct cgttcacggg
    10441 ccggttctgg gttatggagc agaagagcgg gcccacgggc gattcgcaga ccggcctgat
    10501 gggcgagcag cccgaacccg gcgagctccg tcgctggacc tggcaggccg tggcgaacgg
    10561 cgccgatggc gtgtgttatt tccgctggcg ggcctgtctg tccggcgcgg agcaatattg
    10621 gcacgggatt ctggatcacg acggtttggt gcggcgccgg tatcgggaag tcgccgaaac
    10681 gggg
//
LOCUS       DAOPBQ010000049        10639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6056_length_10639_cov_4.297525, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000049 DAOPBQ010000000
VERSION     DAOPBQ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10639
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6056_length_10639_cov_4.297525"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1868)
                     /locus_tag="PKW60_02855"
     CDS             complement(<1..1868)
                     /locus_tag="PKW60_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPJ98403.1"
                     /translation="MTDQIRTSSFPGRLDRFRYFPFGLPVSRRADETFQLKALLEHSR
                     AAGSGFLLRAAQELAYVMNNRRAAESPARPAIRPGVLLTAGLLQDLLRYLIDLYCLDE
                     QPASMASGLSAVAETSKPASVQQSFTAFLELYPPHFDTMAQHCAEAMERAGSPDTQAH
                     YVLGRELVLLELAMNNPAMESIREVFDDTDLCHRAPYRALVGSLERHFEGQPAVSQLG
                     KPLFACLRAPILASPYSLEGQLQYIRQHWGAFLPLPLLERLTRAEDVLKEETRHRGLG
                     PGPAAVLEFGRYAYGGDSGYPEPERFTADRDWMANVALMAKSTYVWLDQLSKKYGRLI
                     RRIDDIPDEELDLLARWGFTGLWLIGIWERSIASQTIKERMGNPEAAASAYSLYDYEI
                     AADLGGPAAAENLRHRAAVRGIHLACDMVPNHVGIYSRWVVEHPDWFIQRDHPPFPVY
                     RFSGPNLSPDPRVEVYIEDGYWERRDAAVVFKRVDRQTGHTRYIYHGNDGTSMPWNDT
                     AQLNYLRQGVREAVINTIMHVAQVFPIIRFDAAMTLSKRHYQRLWFPLPGEGGAIPSR
                     AEYGMAREEFDRHMPQEFWRELVDRVNAQRPDTLLLAVAFLLMEGYFVRTLGMHRV"
     gene            complement(2025..2921)
                     /locus_tag="PKW60_02860"
     CDS             complement(2025..2921)
                     /locus_tag="PKW60_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPJ98404.1"
                     /translation="MSNILIPLGVVCAVAMSPAPVAPSGRELRISKGVFAVVDGRSLG
                     MTIYEPRQRGAGLRPGMVLIHGGAWRFGPRWQQRWYCLAFARAGYVVMSIDYRLMPKY
                     PFPCCLHDCKAAVRWLRLHAEEYEVDPERIVAFGASAGGHLAALLAATGPEDGLEGDA
                     NPGASSAVRAAVSLYGAVDLTKYRDLGLPGLSGKRVAKGMLKFAGTEVAKEGEDPLAA
                     ASPITYAKPEMAPVFLAHGMSDILVHYEQSEAFFARLQELGVPARLVSYPHRNHGFDY
                     IHWKQRRAMFQEMLEFLNEHNQ"
     gene            3061..4584
                     /locus_tag="PKW60_02865"
     CDS             3061..4584
                     /locus_tag="PKW60_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98405.1"
                     /translation="MNRHLSRYRIASLAAALLMCVFLTQCGGLTQKDFEKVNVSGFDA
                     EDQKIDHNDYANSMEYFKADEAEDGYLYVGTCNNMGGLIGFYVQMMRDGGNILDAPIC
                     PPEIRRYRPDIGPLEWETVLDYRDVEEEGSFKTIGFRFMTILEAEELDKDAPPANFLY
                     AATQGETCDLWRSKTGNYGEWDLVYTFEEFGGSIRWIEQFEGRIYLAIAYDSFGIAPP
                     PGEIWVSDDGLDFRPIMRNGFGDPNNRGVQALIAYNGWLYAGTKNDIDGFDIWKLRPV
                     TPGSEEYEYVRVVSGGGPSSHNENAGMPIIFQDKLYFGTQLYIAGINPTSGNAFEGCD
                     IIRINEDDSWDTIVGPRSLSGYASGFNHFTNSYLWWMEEHDGWLYASTFDQGTLLAGL
                     LANLEGVMDFFQKPQDKSTITTVSRLLNAFFDMDDFYRFTHAGGDIFKSPDGVKWYPV
                     TMDGMGNPRNYGWRTMKSCPDGYLYVGSTNTTTGTEIWRGKVPQREKATAQLLQQFK"
     gene            complement(4647..4787)
                     /locus_tag="PKW60_02870"
     CDS             complement(4647..4787)
                     /locus_tag="PKW60_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98406.1"
                     /translation="MRRIKTLTRPKVNYALVHQGLVLTILEKQLRDMLSNLRDTMDKA
                     EE"
     gene            5017..6075
                     /locus_tag="PKW60_02875"
     CDS             5017..6075
                     /locus_tag="PKW60_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPJ98407.1"
                     /translation="MSTNPYENLARVIRHEHADWIPFTLDVGATPGFTGPVMEVFREQ
                     TGAEHPEEYFGYDFRTASLATRFGGPDPAALHASVEPGTAFDEWGIGHWAGGAAGTYE
                     KTYPPLAAASQIREIEAHPSPIIDAGRTASAVEALHARGYPVFGYAGSIYEWSWWLRG
                     MEQFMMDLLAAPKLAEAVIRKVADYTKALALESARAGIDVLCFYDDAGMQTGLQIDPA
                     LWRRFIQPRWREVLEAVRSQFPETWFFLHSCGRIEEIIPDIVDTGFHILHPIQPECMD
                     YAKIRRAFGRDIVLCATISAQTLFPFGTPHEIRNWVRDIRQLCAADNCAILCPSNLVQ
                     PETPWENIVAFAEAARAS"
     gene            complement(6109..7143)
                     /locus_tag="PKW60_02880"
     CDS             complement(6109..7143)
                     /locus_tag="PKW60_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98408.1"
                     /translation="MWEQLRAYDADGVELTCGQGTVLIPPALGGRIFCGLSGELVHRL
                     DVEALRHPSPTEYDNLGGNSLWPAPEGGPFAFNYLSEDGAWTVQDGIAKAVPQVSFED
                     DSSVRIEKRIALTNRKGTQLDLNYRRLVWARALPPAAGHLGLEGLVYDTLDVFEPLGD
                     YDAESALLAPWSLEQFPGADGIIAFGKVPENDAALNRDFYGDPGERIAWRPDHFVFRL
                     GGKARHQIGVRVEHGPQCIGALDLGREMLFVRKTRPQQGTYFNIADNEQPDGPFSTAD
                     LYSIFNGGELDFFELETIGAMQTANGRLATSSLLSQTVILRGDPRKLLAFLLETEGLQ
                     LDESLLDASV"
     gene            complement(7172..8338)
                     /locus_tag="PKW60_02885"
     CDS             complement(7172..8338)
                     /locus_tag="PKW60_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPJ98409.1"
                     /translation="MGYLSQEERDIRLAKVQEILKAKNLDLALVYYDEFNIGNGWYLT
                     GWCPQFESGAVLVLKDGSAMLLGGPESEPFAKQDSAIKETRNFPVFMVPDEEYPNATI
                     TDFPTLFAELSAKEGAIRRAGLVGGGRMPADCFRQITEGFRGVELVDITDAFVALRYD
                     KSAWEREQIRAAFQLADASYDAMLKAIKPGAMEIEVAAAGEYAARSRGATGFGFSAIV
                     GSGARANAVVPTATTKPLEAGELVMVGLAPKVKGYAGVVGHSLPVSGSYSASQAEMLG
                     HLKEVFRLTRAQLMPGRKGCEIDAPGRAYYEKHGLLKYLVCPFAHTIGLHEAEAPFFG
                     PNSQDALRPGMAVCVDVSFFGHPEWHGARIETGYEITEVGPVPFSAKMDRLLTT"
     gene            8575..8649
                     /locus_tag="PKW60_02890"
     tRNA            8575..8649
                     /locus_tag="PKW60_02890"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8607..8609,aa:Gln,seq:ctg)
     gene            8680..8755
                     /locus_tag="PKW60_02895"
     tRNA            8680..8755
                     /locus_tag="PKW60_02895"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8714..8716,aa:Glu,seq:ctc)
     gene            complement(8849..>10639)
                     /locus_tag="PKW60_02900"
     CDS             complement(8849..>10639)
                     /locus_tag="PKW60_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HPJ98410.1"
                     /translation="ADALAPEALAGRIYPAGTPESYLFSGEHAYALKQKRLGLLSSAE
                     ASIVPGAKHCFTFVVAWHFPNQENGHYYAAQFADAAAVANYALDHHERLTADTRLWRD
                     TYYDGSLPYWLLDRLHSTLSYLATGTCQWWKNGRFYAYEGVTCCEGNCTHVWNYAHGQ
                     ARLFPELSRSVREMQDFNPREHGGGFHADTGLVGFRSNDAYAADGQCGTVLKAYREHL
                     MSPDSAFLERNWPRIKLALEYSIGRDGNADGLIEDEQHNTYDINYHGANTFVGSLYLA
                     ALRAGEEMAKEMGDTAFAERARAIYESGRELTVKRLWNGEYFVQDVDLEEYPRHQYKD
                     GCLSDHLFGQGWAHQLGLGYIYPQENVRSALQAVWKYNWAPDISLYNDEHPPFRWFIS
                     PGQAGLLTCTWPRSEFLPEGTMYKSEVWTGIEYQVAGHLAAEGFVEEALVICRAVHDR
                     YSPSLKNPYNEVECGDHYARALASWGVYLSLAGFEYHGPKGYIGFSPRITPDAFKAAF
                     TWAEGWGTFEQTRTARVQTERIDLKWGRLRAKTFAFALEEGVSPGSVTVTAAGRPIGA
                     SFEIKDGLALVALARDATVQQGEAFEIVIA"
BASE COUNT         2115 a         3456 c         3006 g         2062 t
ORIGIN      
        1 taaacacggt gcatgccgag cgtgcgcaca aaatagcctt ccataagcaa gaacgctacg
       61 gcgagcagca gcgtgtccgg ccgctgggca ttcacccggt cgaccagctc gcgccagaat
      121 tcctgcggca tgtgccgatc gaactcctcg cgggccatac cgtactcggc tcgcgacgga
      181 atcgcgccgc cttccccggg caagggaaac cacaagcgct ggtaatggcg tttggagagt
      241 gtcatggccg cgtcaaaacg gatgatcgga aagacctgtg ccacgtgcat gatggtgttg
      301 atcaccgctt cccgcacgcc ctggcgcagg taattgagct gggccgtgtc gttccacggc
      361 atactggtgc cgtcattgcc gtggtagata taccgggtgt gtccggtctg acggtccacg
      421 cgcttgaaca ccaccgcggc gtcgcgccgt tcccaatagc cgtcttcgat atagacttcc
      481 acccgcgggt ctggcgagag attcgggccg ctgaaccggt acacgggaaa cggaggatgg
      541 tcgcgctgaa taaaccagtc cgggtgctcg accacccatc gggaataaat gccgacatga
      601 ttgggcacca tgtcgcaggc caggtgaata ccgcggaccg ccgcccggtg acgcaggttt
      661 tcggctgccg cagggccacc cagatcggct gcgatctcgt aatcatacag ggagtacgca
      721 gacgccgcgg cctccggatt gcccatccgc tctttgatgg tctgcgaggc gatggatcgc
      781 tcccagatgc cgatgagcca gagtccggta aatccccagc gagccagcag gtcgagttct
      841 tcgtctggaa tgtcatcgat ccgccggatg aggcggccat atttttttga aagctggtcc
      901 agccacacgt aggtggactt ggccatgagg gccacgttgg ccatccagtc ccggtcggct
      961 gtgaagcgtt ccggttccgg gtaccctgaa tcgccgccgt acgcatagcg cccgaattcg
     1021 agtactgcgg ccggcccggg acccaggccg cgatggcggg tctcttcctt caacacatcc
     1081 tcggcgcgcg tcagccgttc cagcagcggc agcggcaaaa aggcgcccca gtgctgccgg
     1141 atatactgca actgaccctc gagtgaatac ggagaggcca ggatgggcgc gcgcaggcag
     1201 gcgaacaatg gcttgcccag ctgcgacacg gcaggctggc cttcgaaatg gcgttccagg
     1261 ctccccacca gcgcccggta cggtgcacgg tggcaaagat ccgtgtcgtc aaagacttcg
     1321 cgaatggatt ccatggcggg gttgttcatc gccagttcca gcagaaccag ttcccgcccc
     1381 agaacgtaat gcgcctgcgt gtccgggctg cccgcccgtt ccatggcttc agcgcagtgt
     1441 tgcgccatcg tgtcgaaatg cggcggatac agttcaagaa atgccgtaaa cgattgctgg
     1501 acgctcgccg gtttactggt ctccgcaacc gcggacagtc ccgaggccat gctggccggc
     1561 tgctcatcaa ggcagtacag atcaatcaga taccggagca ggtcttgaag cagcccggcg
     1621 gtcaagagca cgccggggcg gatagccggc cgggccgggc tctcagcggc tcgccggttg
     1681 ttcatgacat aggccaactc ctgggcggcc cgtagaagaa agccgcttcc tgcagcacgg
     1741 ctgtgttcca gaagtgcctt gagttgaaac gtctcatcgg cccggcgaga cacggggagg
     1801 ccgaacggaa agtagcggaa ccggtccagc cggcccggga aagacgacgt acgtatctga
     1861 tcagtcataa ggccatccgc aagggttttg gccggcgctc cagcggaaaa ccggccgaac
     1921 accactgcct tcgtgtaatc gatgccgagt cataaggcgg cagaatacaa catccccggg
     1981 tgcgtttgcc aaagagcagg cgccgctgcg ccctgcctgc cggtttactg gttgtgctcg
     2041 ttcagaaatt ccagcatctc ctggaacatc gcccggcgct gtttccaatg gatgtaatca
     2101 aacccgtggt tccggtgagg atagctcacc aggcgcgccg gtacgcccaa ttcctgcagc
     2161 cgggcaaaga aggcctccga ttgttcgtaa tgcacaagta tgtccgacat cccatgggcc
     2221 agaaacaccg gcgccatttc cggcttcgca taggtgatgg gtgacgcagc cgccagcgga
     2281 tcttctccct ctttcgccac ctcggttcca gcgaatttca acatcccctt cgccacgcgc
     2341 ttgccagaca ggcccggcag gcccaagtcc cggtatttgg tcaggtcgac cgccccgtac
     2401 agcgacaccg ccgcccgcac ggcgctcgac gccccgggat tggcgtctcc ttccaggcca
     2461 tcctcgggac cggtggcggc cagtaaggcc gccaggtgcc cccctgccga cgccccaaac
     2521 gccacaatgc gctccggatc cacctcgtac tcctccgcat ggaggcgcag ccagcggacc
     2581 gccgctttac agtcgtgcag gcagcaggga aacgggtatt tcggcatcag ccggtagtcg
     2641 atcgacatca cgacatagcc cgcccgggcg aacgccaggc aataccagcg ctgctgccag
     2701 cgggggccga agcgccatgc cccgccatgt atcagcacca tacccggccg gagtccggcg
     2761 ccgcgttgcc gtggctcgta gatcgtcatc cccagggaac ggccatccac gaccgcgaac
     2821 acgcctttgc tgatacgcag ttcccggccg ctcggcgcca ccggagccgg gctcattgcc
     2881 acggcacaaa ccacgcctaa aggaatgaga atgtttgaca taccatttct tccgagtctc
     2941 tcccggcaat gggccgaggc aattaagggt tgaccttcgt tttacttcca gttaagattt
     3001 tgcgtcggaa cttggaaact gagaaacctc aacttcgtcg tacagacgcg cgggggtacc
     3061 atgaacagac atctttccag ataccgcatc gcttcgttag ctgcagcatt gctgatgtgc
     3121 gttttcctga cacaatgcgg cggcctgaca caaaaagact tcgaaaaagt caacgtcagc
     3181 ggcttcgatg ccgaagacca gaaaatcgac cacaatgatt atgccaattc catggaatac
     3241 ttcaaagcag acgaggccga agacggctat ctctatgtcg gcacatgcaa caacatgggc
     3301 ggcctcatcg gcttctacgt gcagatgatg agggacggcg gcaacattct cgacgcgccc
     3361 atttgcccgc cggagatccg gcgctaccgc cccgatattg gcccgctgga atgggaaacc
     3421 gtcctcgact accgcgatgt cgaagaagaa ggcagtttta agaccattgg cttccggttt
     3481 atgaccattc tggaggccga ggaactggac aaagacgcgc cgccagccaa cttcctctat
     3541 gccgccaccc agggtgagac atgcgacctg tggcgctcca aaaccgggaa ttacggcgaa
     3601 tgggacctcg tgtacacgtt tgaagagttc ggcggatcca tccgctggat cgaacaattc
     3661 gaagggcgta tctatctcgc catcgcctac gacagtttcg gcattgcccc gcctcccggc
     3721 gaaatctggg tgtcggatga cggtctcgat ttcaggccga tcatgcggaa cggcttcggc
     3781 gatcccaaca accgtggcgt ccaggccctc atcgcctaca atggctggct gtacgccggt
     3841 accaaaaacg acatcgacgg attcgacatc tggaagctgc gccccgtcac gccgggctcc
     3901 gaagagtacg agtatgtgcg cgtcgtcagt ggcggcggcc ccagctcgca taacgaaaac
     3961 gccggcatgc ccatcatttt ccaggacaaa ctctacttcg gcacccagct ctacattgcc
     4021 gggatcaatc ccaccagcgg caacgccttc gaagggtgcg acattatccg catcaacgaa
     4081 gacgactcgt gggataccat tgtcgggccg cgttccctct ccgggtatgc gtccgggttc
     4141 aaccatttca ccaactcgta cctctggtgg atggaagagc acgacggctg gctttacgcc
     4201 tcgacgtttg accagggtac tcttctcgcc gggctcctcg ccaatctgga aggcgtgatg
     4261 gatttctttc agaagccgca ggataagagc acgatcacga ccgtcagccg cctcttgaac
     4321 gccttttttg atatggacga tttttaccgg ttcacccacg cgggcggcga tattttcaag
     4381 agccccgacg gcgtaaaatg gtatccagtc accatggacg gcatgggcaa ccccagaaac
     4441 tacggctggc gaaccatgaa gtcctgccct gacgggtatc tctacgtcgg ctcaaccaat
     4501 accacgaccg gcaccgaaat ctggcgaggc aaggtgcctc agcgcgaaaa agctacggcc
     4561 caattgctcc agcagttcaa ataacacgct ggaggcatgg gccgtatcaa aaaagctccg
     4621 gtcgcccggg cgagtggctt cagcggctac tcttccgcct tatccatggt gtcgcgcagg
     4681 ttggacaaca tatcgcgcaa ctgtttttca agaatagtca ggaccaggcc ttgatgaacg
     4741 agcgcgtagt ttactttcgg ccgtgtcaac gttttgattc gcctcatgtt ctgttcctcg
     4801 gttttgttaa tgcggaccag tttttgccgt aaacgcacgg cagtataaag agccgggcgc
     4861 tgccgtgcaa ctataaaaag cggcgcgcaa tgcacgcggg gcgcgtgcat caaaatgagg
     4921 gcagagcacc gagcctttca gctggaggtt cggcggtggc aaaatcggac ggagacacgg
     4981 ccgatttcta cagatccggc cacatggagt gcggtcgtga gcacgaaccc ttacgaaaac
     5041 ctggcgcgag tcatacgcca tgaacatgcc gattggatcc cgtttactct ggacgttggc
     5101 gcgacgcccg gtttcaccgg ccccgtcatg gaggttttcc gcgagcagac cggggcggag
     5161 caccccgagg agtatttcgg ctacgatttt cggacggcct cgctcgccac gcgcttcggc
     5221 gggcccgacc cggccgccct gcatgcgtca gtcgaacccg gaaccgcgtt tgacgaatgg
     5281 ggtatcggcc actgggccgg cggtgcggcc ggcacttacg aaaaaacgta cccgcccctg
     5341 gcggcggcct cccaaatccg tgagatcgag gcgcatccgt cgcccataat cgatgcaggc
     5401 cgcacggcca gcgcggtcga agcgctgcat gcccggggct atcctgtgtt cgggtacgcg
     5461 ggcagcatct acgaatggtc ctggtggctg cgtggcatgg aacagtttat gatggacctg
     5521 ctcgccgcgc cgaaactggc ggaagccgtt atccggaaag tggccgatta caccaaggcc
     5581 ctcgccctgg aatccgcacg cgccggaatc gatgtcctgt gcttctacga cgatgccggc
     5641 atgcaaaccg ggctgcagat cgaccccgca ctctggcggc ggttcatcca gccgcgctgg
     5701 cgcgaggtgc tcgaggccgt ccggagccag ttccccgaga cctggttttt ccttcacagt
     5761 tgcggccgga tcgaagaaat catccccgac attgtcgaca cagggttcca cattcttcat
     5821 cccattcagc ccgagtgcat ggattacgca aaaattcgcc gcgcattcgg ccgggacatc
     5881 gttctgtgcg ccacgatcag cgcccaaacc ctcttccctt tcggcacacc gcacgagatt
     5941 cgcaactggg tacgggacat ccggcagctc tgcgctgccg ataactgcgc catcctgtgc
     6001 ccgtcaaatc tcgttcagcc ggaaacgccc tgggaaaata tcgtcgcgtt cgccgaagcg
     6061 gcgcgtgcgt cctgagaaac cctctttcat gcggcctgtc cgaacccctc acacggaggc
     6121 gtccaggagt gattcatcga gctgcagccc ttccgtctcc agaaggaagg cgagcagttt
     6181 tcggggatct ccacgcagga tcacggtctg cgacagcagg gagctggttg caagccggcc
     6241 gttcgcggtt tgcatggccc caatcgtctc cagctcgaag aaatccagtt ccccgccatt
     6301 gaaaatactg tacaggtccg ccgtcgaaaa cggcccgtcc ggttgttcgt tatcggcaat
     6361 attgaaatag gtcccctgct gcggtcgtgt cttgcgtaca aacagcattt cccggccgag
     6421 gtccagtgcg ccgatacact gggggccgtg ctctactcga acgcctatct gatggcgcgc
     6481 cttgcctcca agccgaaaaa caaaatgatc cggccgccac gcaatccgct cgcccggatc
     6541 cccgtaaaag tcacggttca aggcggcatc gttctccggc actttcccaa aggcaatgat
     6601 gccatccgcg ccggggaact gctcgagcga ccacggcgcc agaagcgcgc tctcggcatc
     6661 gtagtctccc agaggttcga agacgtccag cgtatcgtag accagcccct ccagccccag
     6721 atgaccggcg gccggaggaa gcgcccgggc ccacacgagc ctgcggtagt tcaagtcgag
     6781 ttgcgtgccc ttccggttcg tcagcgcgat ccgtttttct atccgaacgc tgctgtcgtc
     6841 ctcaaacgag acctgcggca cggccttcgc aatgccgtcc tgcacggtcc acgctccgtc
     6901 ttccgacaag taattgaacg caaacggccc gccttcaggc gccggccaga gcgagttgcc
     6961 tcccagattg tcgtattctg tcggggaggg gtgccgcaac gcctcgacat caagccggtg
     7021 caccagctcc ccgctcaatc cacagaaaat gcgcccgccc agcgccggag gaatcaaaac
     7081 ggttccctgg ccgcatgtaa gctcgacccc gtccgcgtca tatgcccgca gctgttccca
     7141 catcttcatg cctcctcagc gacgatgccc gtcatgttgt aagcaaccgg tccatcttgg
     7201 cgctgaaagg cacggggccg acttcggtga tttcgtagcc cgtctcaatc cgggcgccgt
     7261 gccattccgg atgtccgaag aaacttacat cgacacagac cgccatgccc ggcctcaggg
     7321 catcctggct gttgggaccg aagaaggggg cttcggcctc atgcaggccg atggtgtgcg
     7381 cgaaggggca taccagatat ttcagcagcc cgtgcttttc gtaataggcg cgacccgggg
     7441 cgtcgatttc gcagcccttt cgcccgggca tcaattgggc ccgggtcagc cggaatactt
     7501 ctttcagatg acccagcatc tctgcctgcg atgccgagta gctgcccgac accggcaggg
     7561 aatggcccac gacacccgca tagcctttca ctttcggcgc caggcccacc atcaccagct
     7621 cgccggcttc cagaggcttc gtcgtcgccg ttggaacgac cgcgttggcc cgcgcgcccg
     7681 aacccacaat cgcgctgaac ccgaagcccg tagcaccacg gctgcgcgcc gcatattcgc
     7741 cagccgccgc gacctcaatt tccatggcgc ccggtttaat cgccttcagc atcgcgtcat
     7801 aactcgcatc cgcaagctga aacgccgcac gaatctgttc ccgttcccac gcggacttgt
     7861 cgtagcgtaa ggccacaaac gcatcggtga tatccacgag ttccacaccg cgaaacccct
     7921 cggtaatctg ccggaaacaa tcggcaggca ttcgtcctcc gccgaccaga cccgccctcc
     7981 ggatcgcccc ttccttcgcg ctgagttccg cgaacaaggt cgggaaatcc gtgatcgtcg
     8041 cgttggggta ctcctcgtct ggaaccatga acaccggaaa attccgtgtt tccttgatcg
     8101 cgctgtcctg cttggcgaat ggctcactct ccggaccgcc caacagcatc gcgctcccgt
     8161 ctttcaacac cagcaccgcg ccgctttcaa attgcgggca ccacccggtg agataccatc
     8221 cgttgccaat gttgaattcg tcgtagtaca ccaaagccag atccaggttt ttggctttca
     8281 ggatctcctg cactttggca agccggatgt cgcgctcttc ctgactgaga taccccattt
     8341 ccgcgcctcc ttatcaaact cgttccaggt gggtggcagg caacgcgacg ccaaccccgt
     8401 caccccggca cggaccttat tggctcacca cgcacgccgc cgccatttct cccatcatac
     8461 tccagctcaa tcgatcattt cctccagaat cgtccgcatg aacccgcggg gcgatgcacg
     8521 cgcggcctct ctgtttgact cccccggccg gtttacgcta gaattccgtt cagttgccgg
     8581 atagcctaat ggtagggcac ctgactctgg atcagggtag tctaggttcg agtcctagtc
     8641 cggcaaccaa acacgagttt aatcgttcgt ttatagcgtg gcccgttcat ctagtggtct
     8701 aggatgcagg attctcagtc ctgtcacagg ggttcgagtc ccctacgggc taccatacct
     8761 ctacaaaagg gcccgcgtac cggcatgcgg gcccttttgc tgtctctcaa agccgttttt
     8821 ccagcaatcc gtgcggacca tgcggcattc atgcgatcac aatttcgaac gcctcgccct
     8881 gctgtaccgt cgcatcccgt gccagcgcca caagcgccag gccatctttg atttcaaacg
     8941 acgcgccaat cggcctgccg gcggcggtta ccgtgaccga ccccggtgaa acgccctcct
     9001 ccagcgcgaa cgcgaaggtc ttcgcccgca gccggcccca cttcaaatcg atgcgttcgg
     9061 tctgaacccg cgccgtccgc gtctgctcaa acgtccccca tccctcagcc caggtaaagg
     9121 ccgctttgaa cgcatccgga gtgatccggg gcgagaatcc gatgtagccc ttcgggccgt
     9181 ggtattcaaa tcccgccagt gacagataga cgccccagct ggccagcgct cgagcgtagt
     9241 gatccccaca ctcgacctcg ttgtatggat ttttcaggct gggactgtag cggtcgtgca
     9301 ccgcgcggca gatgaccagg gcctcttcca cgaatccttc agcggcgaga tggcccgcca
     9361 cctggtattc gatgcccgtc cagacctcgc tcttatacat tgtcccttcg ggcaggaact
     9421 cgctcctggg ccaggtgcag gtcagtaatc cggcctgccc cgggctgatg aaccagcgga
     9481 acggaggatg ttcgtcgttg tagagcgaga tatccggcgc ccagttgtat ttccagaccg
     9541 cctgaagcgc cgaccggacg ttctcctgcg gatagatgta gcccagaccc agctggtgcg
     9601 cccatccctg gccaaaaagg tggtccgaca ggcatccgtc cttatactga tgccgcggat
     9661 actcctcgag gtcgacatcc tgaacaaaat attcgccatt ccacaggcgc ttgaccgtga
     9721 gttctcttcc gctctcgtag atcgcgcgag cccgttcggc aaacgcggta tcccccattt
     9781 cctttgccat ttcctcgccg gcccgcaacg ccgcgagata cagcgagccc acaaaggtgt
     9841 tcgcgccatg gtaattgatg tcgtaggtgt tgtgctgctc atcttcgatc agcccgtcgg
     9901 catttccgtc acggccgatc gaatactcga gcgccagttt gatcctgggc cagttccgct
     9961 cgagaaacgc gctgtccggc gacatcagat gctcgcggta cgctttcagg acggtcccgc
    10021 actgcccgtc cgcggcatag gcatcgttgc tgcggaatcc caccagcccc gtgtccgcgt
    10081 gaaaccctcc gccgtgctcg cgtggattga aatcctgcat ctcgcgaacg cttcgcgaca
    10141 gctctggaaa caggcgcgcc tggccgtgcg cgtaattcca gacatgggtg cagtttccct
    10201 cacagcaggt cacgccttca tacgcataga aacggccatt cttccaccac tggcaggtgc
    10261 ccgtcgccag atacgacagg gtcgaatgga gccgatccag cagccagtac ggcagcgagc
    10321 cgtcgtaata ggtgtcgcgc cacaaacgcg tgtccgccgt aagccgttca tgatggtcga
    10381 gcgcatagtt cgccacggct gccgcgtcgg cgaactgcgc cgcgtagtag tgtccatttt
    10441 cctgattggg gaagtgccag gcgacgacaa acgtgaagca atgcttcgcg cccggcacta
    10501 tcgaggcttc ggccgaactc agcaggccga ggcgcttctg cttgagcgca tacgcgtgtt
    10561 ccccgctgaa cagatagctt tccggcgttc ctgcggggta gatccggccg gcaagggctt
    10621 cgggcgccag ggcatccgc
//
LOCUS       DAOPBQ010000050        10579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6108_length_10579_cov_4.334474, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000050 DAOPBQ010000000
VERSION     DAOPBQ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6108_length_10579_cov_4.334474"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3886
                     /locus_tag="PKW60_02905"
     CDS             <1..3886
                     /locus_tag="PKW60_02905"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPJ98411.1"
                     /translation="ILPEQSPRRYTAAMAARFLQEGRITAGLEHPSIVPVYEIGRRES
                     GNLYYTMKLVRGRSLSHTLSACANLEERLALLPHFVDLCEAIAYAHSRGVLHRDIKPS
                     NVMIGEFGETVVLDWGLAKIKAQAEDPLEQQIRETPRFFADKTMPLPETRAGQAIGTP
                     EYMPPEQVQGAAEKIDERSDVYSLGVVLYEFLTGTTPFRSDSVSETMRRVVVEVAQPA
                     REKETDAPAELVSICEKALRKAPEERYQSVRELADEVKRFQTGALVQTYRYSLGELAR
                     RYYKRHRAVLNVAAAAAAVVLGLAVFSYGQIYQARNREHDQRLLAENARDAAIIAEDK
                     AEKAREEAEHTAYVAQIRLLDAYVRNRNFARANRLVWEIAEPHRNWEWGYLLEQCNQD
                     HFTLRGHHGILFRAIMDRRGKRILTLAGDRTARIWDADSGSPIVEIKTPYALINDGQF
                     SPDESSVALALWDGTARLFDAFSGEEIMTFKGHTASVRSVRFFLDTDWLLTASNDGTL
                     RIWSTTTGTQIDCLEDPGSEIMGAYPSPGRTRFVSATSTGMLRLWDLSLSDEPLRSMP
                     GTLPVYSASGILICFVDNGAAVVWDSGADSELLRIEPGTAQVLRVRFSPDDRRLVTAA
                     TDGIARVYDVETGALLNSFNHGEEIADAVFSPNQRLILLRSDAGLITVWDADSGARIA
                     SFTGHEAGIAAAVFSPDSQHVITASRDHTARVWDVYRPLTHRAVVEQSSPAVRVAVSG
                     DGERCAVATQSCVLSVCDGRTLQPMPAHWCFSHFGAASVALSSDGALLAAPLDGFTVF
                     VLDTSTGKALSTYTGHRGRVYATAFSPDSSSVLTASRDGTAQIWDAGNAQTHTVLKGH
                     SKPLRCAAFSPDGSVVATGGDDATIHLWNAQTGAEIQTLNKHTGSVNAVAFDATGKRL
                     LSASNDGTVLCWDLARREAQVQFLGHSDQVASAQFSPNGERILTASWDKTCRVWDAAT
                     GDELLSFGTGEHPFLDAAFVRGSRNVLTASWDGCVTAHFAGPWHREQLEGNPGDSFQA
                     RYEAARPSRLEERWPDPDQAAPGAKAFVHTTYRRYYERFTLLYDLIPDEDAAASAHDM
                     VITPGAVADAAKRLCLLPGDQITRINDTRITDRASFIDALSTFILDMDEPPRDKLEIV
                     RGGTPVELNYIFYPQIEIEQPVTLSREAVGECINYLEDVLILGIEQLLRVNQDMNTRL
                     GEPVSERDSLNGLWIPPETGDQGPAILEMLGIAVGDRILSLNGTRIESLSQLEEFIRA
                     VKSKLATNDPIRVDATIERGEFQVVKLTIEVT"
     gene            4016..4822
                     /locus_tag="PKW60_02910"
     CDS             4016..4822
                     /locus_tag="PKW60_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98412.1"
                     /translation="MSGWSFNILTPKYTPWEAGTYKLRAGCWEIGLMCHLLNQFNDYS
                     HLYGMKVAHEKKQDALARLAQGVEAIPEGELSKQTRDAIIAELNNLRQAFLKAHPNKT
                     MRDLYTWPTAEAVDPEIVKGFENIEALVAQDTESQKDPMTGGARFSRPCTPCMPFLAV
                     LKKEHPPVPPEAMDEHIANVGEHTLEELYKMQKTLANKPKWETVSKAKKNGIFARPGD
                     ITACLGSGSPSDERGKDPGNDLKCINGRCQPHIDTFCSIAEDGGCSAMSD"
     gene            4958..7459
                     /locus_tag="PKW60_02915"
     CDS             4958..7459
                     /locus_tag="PKW60_02915"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HPJ98413.1"
                     /translation="MKYATGVAAILLAMTFFGTVQANADPQEIYVRLFGDAAHLEPDI
                     HRQVCEDEPGKRHYIDRDQDGRPEEVWFIDTDLRHPEARRPVLVRVIDRDGDLRMGDE
                     PDYDSDLYLADWNADGTVDVVIEYMDLDGDNDVDAMAMYFPRGEGVMCWYAEDIGDDN
                     LLWYDVAYTYDQRLCQGRTHFGGNELFCAFILEPGSSEWRPQWENPFAFYDHDLDGVS
                     EEVIRIEGLDDTVKNLRYSFDADKDATWEHPRDFDVSITAHAPEGLRFEPQYATRRTL
                     CNLPTGPFLNYYALPAWALGAEWATRMLTWDEIDRNIDRDRSEDTGERWEGVIAKGND
                     AFPQVGGPSSGPFNNRFELASSPGRIRVYYAPTDQRIHLYGADTQWLEVDFDHDGVPD
                     MRYSYVDSNGDGYIDVWELDADADGVVDDRWTINAAFLDLAYNWGQVHAIMRPLLDTV
                     PEQLFLFIARLREALATKNAAPDDPVCALLDSGFAIDTISPEFRKDFLESNESLRFYF
                     DLAKDRLIFALKQAFDDPAFWEKLDGLRGAGDIEAMRQLVEETFQCGTPLPGFESWRT
                     ERLAALKSPRVAWAQDWVPPNIGWESELAGYRVYWGQFDFFGKHQPALVMHTFGGGIN
                     YHAEQPWGMDALHVNETGGLGGVTLYINGQPYPVYSPGGKGPITWSKRLVDIDDDRVT
                     VEVLAENVGPEDAPCTVRFFCSALAGRRDSPIELVVEGGPSGATLETGLGIVRMPQET
                     FALDTASGVMGSWGVQGTDIGWIGLGVVFPPERYRRAVDSDTEHQVILDARPGTPLQY
                     HIQGDWLRGRRFPRSPVLSNWMDDLRRTARLANLK"
     gene            7563..8297
                     /locus_tag="PKW60_02920"
     CDS             7563..8297
                     /locus_tag="PKW60_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPJ98414.1"
                     /translation="MKLRAILLTTVAFVALTSYAMAQDPIEAPQWVSLFNGTDLTGWI
                     NVNTAEDTWSVRDGLLVCKGHPIGVMRSEKQYENFILHIEWRHMEPGGNSGVFVWSEG
                     TVPEGAELPKGIEVQMLELEWVNLNKKPDGELPPIAYVHGELFGANGAVTIPDNPRGS
                     RSKSIENRCKGKGEWNVYDVVCVDGVIKLAVNGKFVNGISGSSVRKGYLCLESEGAEI
                     HFRNIRILELPPGVITPEQTAPVVEP"
     gene            complement(8316..9326)
                     /locus_tag="PKW60_02925"
     CDS             complement(8316..9326)
                     /locus_tag="PKW60_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase"
                     /protein_id="HPJ98415.1"
                     /translation="MRVVGVFLLVFVFGASAFAEKAQPEWVQETEAAAWAPRDSAGEM
                     VYDGRLWLLGGWLQSYEAPPRDVWSSPDGKTWTRVLETAPWIHSDLPMTIAFNDRMWL
                     MGGWYNGRLPGHSASNEVWWSIDGANWQQATAAAGWSPRCAAALVEFQGKMWLLGGTE
                     NYYFGDASSLKNDVWCSSDGEHWVQVTDNAGWPPRAYHQAAVLDGKLYVFGGGNYVPD
                     YSAFNDVWMSEDGKTWTCVTEAAPWHPRLWFSSAVYRGRMWVLGGWSNNPSKNWDDVW
                     YSKDGAVWTPLKAGARWRERHEHSLFVHDDKLWVAGGMIPPLCNDVWSLYLPPDWFSD
                     ED"
     gene            9615..>10579
                     /locus_tag="PKW60_02930"
     CDS             9615..>10579
                     /locus_tag="PKW60_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HPJ98416.1"
                     /translation="MKSPVFTTAKPGILHRLPLLVMLLLAPAASADSTEVWTRWEHRL
                     VSDRAYENPCVDVEVRVLYENAEGETRRGMGFWDGEDAFVIRQAFPRPGDWRWSTECS
                     DPSNQGLQQTGTVRVVPGSARNPLVRHGYLRVSDDARTLSYADGTPFLWVGDTCWAAP
                     AHSTFEEWARYLDSRAAKGYTVLQTSIAPEWALKHSRRGIPPFLSRLPDISQPNPAFF
                     RELDRMFDMANDQGFVVMLCGLMETPYEYPPAGDIAIFSRYVAARYAAHALIFSPSFD
                     SGIHEAETLASALAIREAAPANMVTMHMGTGVGPHFHGADWLAFD"
BASE COUNT         2145 a         3305 c         3168 g         1961 t
ORIGIN      
        1 gatccttccc gagcagagcc ccaggcggta caccgccgct atggcggcgc gtttcctgca
       61 ggaagggcgg attaccgccg gcctcgaaca cccgtcgatc gtgccggtat acgaaatcgg
      121 ccgcagggaa agcgggaacc tgtattacac aatgaaactg gtgcgcggcc gttccttgtc
      181 tcacaccttg agcgcgtgcg ccaatctgga ggaacggctc gccctccttc cccatttcgt
      241 cgacctgtgc gaagccatcg catacgccca cagccgcggc gtcctccatc gcgacatcaa
      301 gccgtcgaat gtgatgatcg gagaattcgg cgagacggtg gtgcttgatt ggggccttgc
      361 caagatcaag gcccaggccg aggatccgct cgaacagcag attcgcgaga cccccaggtt
      421 ctttgccgac aagacaatgc ccttgccgga aaccagggca gggcaggcca taggcacccc
      481 ggaatacatg ccgcccgaac aagtccaggg cgctgcggaa aaaatcgacg agcgatccga
      541 cgtatactcc ctgggcgtcg tcctgtatga atttctcacc ggcaccacgc cgttccggtc
      601 ggacagcgtc agcgaaacca tgcgacgggt ggttgtggag gttgcgcagc ccgcacgcga
      661 aaaggaaacg gatgcacccg ccgaactggt cagcatttgc gaaaaggcct tgcgcaaggc
      721 tcccgaagag cgctaccagt cagttcggga gctggcggat gaagtgaagc gcttccagac
      781 cggcgcgctc gtgcagacct accggtacag cctcggggaa cttgctcgcc ggtattacaa
      841 gaggcaccgg gccgtgttaa acgttgccgc agccgctgcg gccgtggtcc tcggattggc
      901 cgtgttctcc tacggccaga tctatcaggc gcgaaaccgc gaacatgacc agcgtttgct
      961 tgccgaaaat gcgcgagatg ccgcgattat tgcggaagac aaggccgaaa aggcccggga
     1021 ggaggccgaa cacacggctt atgtcgcgca gatccgcctc ctggacgcgt atgtgcgcaa
     1081 ccgtaatttc gcccgcgcca acagactggt ctgggagatc gcagaaccgc accgaaactg
     1141 ggaatggggt tatcttctcg agcaatgcaa tcaggaccat tttacgcttc gagggcacca
     1201 cggcatcctg tttcgcgcca tcatggaccg acggggaaag cgcattctga cgctcgcggg
     1261 cgatcgcacg gcacgcatct gggacgcgga cagcggttca ccgatcgtcg agatcaaaac
     1321 gccgtacgcc cttatcaatg acgggcagtt cagccccgac gaatcctccg tcgcgctggc
     1381 cctctgggat gggaccgcac gcctctttga cgccttcagc ggagaggaaa taatgacgtt
     1441 caaggggcac accgcctcgg tgaggtccgt gcgcttcttc cttgacaccg actggctcct
     1501 tacggcgtca aacgacggca cccttcgaat ctggagcacc acaaccggaa cgcagatcga
     1561 ctgtctcgaa gatcccggca gcgagatcat gggggcttat cccagtcccg gccgcacaag
     1621 gttcgtcagc gcgaccagca cgggcatgct gcgcctgtgg gacctgtctt tgtccgatga
     1681 gccgctgcgc tccatgccgg gcactttgcc ggtatacagc gcatccggga tcctcatctg
     1741 cttcgtcgac aacggagcgg cggttgtgtg ggattcgggc gcggacagcg agttgctgcg
     1801 gatcgagccg ggcacggcgc aggtgctccg ggtgcgcttc tctcccgacg acagacggct
     1861 cgtgaccgcc gccactgacg gaattgcccg cgtgtacgat gtcgagacag gcgctctcct
     1921 caattccttc aatcacggtg aagaaattgc cgatgccgtg ttcagcccga accagcggct
     1981 tatcctcctt cgttccgacg ccggcttgat tacggtatgg gacgccgaca gcggcgctcg
     2041 gatcgcgtcg tttaccggcc acgaagccgg tatcgcggcc gcggtcttca gtccggacag
     2101 ccagcacgtg atcacggcat ccagagatca caccgcccgc gtgtgggacg tatacaggcc
     2161 gttgacgcac cgcgcggtgg ttgagcaatc atcccccgcc gtccgcgtcg ctgtcagcgg
     2221 cgacggagaa cgctgcgccg tcgccactca gagctgtgtt ctctcagtat gcgacggccg
     2281 caccttgcag cccatgcctg ctcactggtg tttctcgcat ttcggggctg cctcggtcgc
     2341 cttatccagc gatggtgcgc ttctcgccgc cccgctggac ggattcaccg tttttgtgct
     2401 cgacacgtcc acgggcaagg ctctgtcaac ctacacgggc caccggggcc gtgtatatgc
     2461 caccgcgttc agcccggata gcagcagcgt gctcaccgcg agccgcgatg ggacggcgca
     2521 gatctgggac gcaggaaacg cccagacgca caccgtgctc aaaggccact cgaaacccct
     2581 gcgctgtgca gccttcagcc ctgacggcag cgtagtggcc accggcggtg acgacgctac
     2641 gatccacctc tggaacgcgc agaccggggc tgagattcag accctgaaca agcacaccgg
     2701 cagcgtgaac gccgttgcgt tcgacgcgac cggaaaacgc ctcctttcgg cctcgaacga
     2761 cggcaccgtc ctttgctggg acctggcgcg cagggaggcg caagtacaat tcctgggcca
     2821 ctcggaccag gtggcttcgg cgcagttcag cccgaatggc gagcggatcc tgaccgcctc
     2881 gtgggacaag acctgccgag tgtgggacgc cgcaaccggc gacgaactcc tgtcgttcgg
     2941 cacgggagaa catccgttcc tggacgcggc attcgtgcgt ggctcgcgca atgtcctgac
     3001 cgcatcctgg gacgggtgcg tcaccgcgca cttcgccggc ccgtggcacc gggagcagct
     3061 ggagggcaat cccggcgatt cgtttcaggc gcggtacgaa gcagcaagac cctcccggct
     3121 ggaagagcga tggcccgatc cggatcaggc cgctcctggc gcgaaggctt tcgtacatac
     3181 gacctacaga cggtattacg aacgcttcac actcctgtac gacttgattc cagacgagga
     3241 tgcggcggcc tccgcccacg acatggtcat cacacccggt gcggtggcgg atgccgccaa
     3301 gcgcctgtgc ctgctgcccg gcgaccaaat cactcgaatc aacgacacgc gcatcaccga
     3361 cagggcatcc ttcatcgacg ccctctccac gtttatcctg gatatggacg aaccaccgcg
     3421 tgataaactc gagatcgtcc gtggcggaac gccggttgag ctgaactata ttttttaccc
     3481 acagatcgag atcgagcagc ccgtgaccct gtcgcgcgaa gccgtcggtg aatgcatcaa
     3541 ctatctggag gacgtcctta tactgggcat cgaacaactc ctccgcgtca atcaggatat
     3601 gaacactcgt ctcggtgagc cggtatccga gcgcgacagt ctcaacgggc tgtggattcc
     3661 tcctgaaacc ggcgaccagg ggcccgctat cctcgaaatg ctggggatcg ccgtgggaga
     3721 ccgcatcctg agcctcaacg ggacgcggat tgagtccttg agccagctcg aagagttcat
     3781 ccgtgccgtc aagtccaaat tggccaccaa tgacccgatt cgggtggacg ccaccattga
     3841 gcgcggggaa ttccaagtcg ttaaattgac gattgaagtg acatagcgca gattggggcg
     3901 cgcaagcgcc agccgtatcc cgtctctcgc ccacggcgtt gaaagaccgg gttcaggaag
     3961 cagcacgaag cggggagccg cgtgaaaacg atacggattc cgaaaggaga gtgtcatgag
     4021 tgggtggagc ttcaatattt tgacgccgaa atacacgccg tgggaggcag ggacctacaa
     4081 gttgcgcgcc ggttgctggg agatcgggct gatgtgccat ctgcttaatc aattcaacga
     4141 ctatagtcat ctgtacggga tgaaggtggc ccacgagaag aaacaggatg ccctcgcacg
     4201 gctggctcag ggcgtggagg ccattccgga gggcgaactc tccaaacaaa ctagggatgc
     4261 gatcattgcg gagctgaaca atctccggca ggccttcctg aaggcccacc ccaacaagac
     4321 aatgcgcgat ctgtacactt ggcccacagc agaagcggtg gatcccgaga ttgttaaagg
     4381 gttcgagaat atcgaggctc tagtggcgca ggacacggaa tcgcagaaag acccgatgac
     4441 cggcggcgcc cgcttctcgc gcccctgcac tccgtgcatg ccgtttcttg ctgtgctgaa
     4501 aaaagagcac ccgcctgtcc cccccgaggc gatggatgag cacattgcca atgtcggcga
     4561 gcatacgctc gaagaactct acaaaatgca gaaaacgttg gccaacaagc cgaaatggga
     4621 gaccgtctca aaagcaaaga agaacggaat cttcgcacgg cctggcgaca taaccgcctg
     4681 cctcggaagc ggttcaccct ccgatgagag aggcaaggat ccgggtaacg acctcaaatg
     4741 catcaacggg cgctgccagc cacacattga tacgttctgc agcatcgccg aggacggcgg
     4801 gtgttccgcc atgagcgatt gagagtaatc cagagcgcca catccagcac ccgatttgac
     4861 agggccgggc tgctttcttt tacagtgaga aaaatctccc cgcggcgcat tccgcgccgc
     4921 aacggtaagc tgctgcacaa aagcacggag aaaacgcatg aaatacgcga ctggagtcgc
     4981 cgccatcctg ctggccatga ctttcttcgg cactgtccag gccaacgccg atccacagga
     5041 aatctacgtt cgtctctttg gcgatgcggc acacctggag ccggacatcc accgccaggt
     5101 ctgcgaagac gaaccgggta aacggcacta catcgaccgc gatcaggatg gacggccgga
     5161 ggaagtgtgg ttcatcgata ccgacctgcg gcatccggaa gcacggcggc cggtcctcgt
     5221 gcgcgtcatt gatagagacg gcgatcttcg catgggcgac gagccggact acgacagcga
     5281 cctctatctt gccgattgga atgccgacgg caccgtggac gtcgtcatcg agtacatgga
     5341 cctggacgga gacaacgacg tcgatgcgat ggccatgtat ttcccgcggg gcgagggcgt
     5401 gatgtgctgg tacgccgaag acatcggcga cgacaacctg ctctggtacg atgtcgccta
     5461 tacctacgac cagcggctct gccagggacg gacgcacttt ggcggcaacg aactcttctg
     5521 cgcattcatt ctggagccgg gcagctccga atggcggccg caatgggaaa acccctttgc
     5581 cttctacgac catgacctcg acggtgtcag cgaagaggtg atccgtatcg aggggctcga
     5641 cgataccgtc aaaaaccttc gctacagttt cgatgcggac aaggatgcga cgtgggagca
     5701 tccccgggat ttcgacgtct ccatcacggc acatgccccc gaaggcctgc gctttgagcc
     5761 tcagtatgcc actcggcgca cactgtgcaa cctgcccacc gggccgttcc tcaattatta
     5821 cgcactgccc gcctgggcgc tgggggctga atgggcgacc cggatgctta cgtgggacga
     5881 gatcgaccgc aatatcgatc gcgacagaag tgaagatacc ggggaacgct gggagggtgt
     5941 gatcgccaaa gggaacgacg ccttcccgca ggtcggtggc ccgagttcgg ggcctttcaa
     6001 taaccgtttc gagcttgcca gctcgcccgg ccgcatccgt gtctattacg cacccaccga
     6061 ccagcgtatt catctctacg gcgcagatac gcaatggctc gaggtcgatt ttgatcacga
     6121 cggcgtgccc gatatgcgct atagctacgt agattccaac ggcgacggct acatcgacgt
     6181 ctgggagctg gatgccgacg ccgacggcgt cgtggatgat cgatggacaa tcaatgcggc
     6241 cttcctggat cttgcctaca actggggtca agttcatgcg atcatgcgcc cattgctgga
     6301 taccgtcccg gagcagctgt ttcttttcat tgcacggctc cgggaggcgc tcgcaacgaa
     6361 gaatgccgcg ccggacgatc cggtctgcgc actgctcgat tcgggcttcg ccatcgatac
     6421 catctcgcct gaattccgca aggattttct cgaaagcaat gaatcgcttc gcttttactt
     6481 cgatctggcc aaagatcgcc tgatctttgc ccttaaacag gcgttcgatg accccgcctt
     6541 ctgggagaaa ctggacggtc tgcggggagc cggcgacatc gaggccatga gacagctcgt
     6601 cgaggagacg tttcagtgcg gcactccctt gcccggcttc gagtcctggc gcaccgagcg
     6661 tctcgccgca ctgaaatcac cccgagtggc ctgggctcag gattgggtgc cgccgaacat
     6721 cggctgggag tcggaactgg ccgggtaccg ggtttattgg ggtcagttcg acttcttcgg
     6781 caaacatcag cccgcgctcg tcatgcatac cttcggcggc ggaataaact accatgccga
     6841 acagccgtgg ggaatggacg cgctccacgt caacgagacc ggcggactgg gtggtgtcac
     6901 gctctatatt aacggtcagc cttatccggt ctacagtccc ggcggcaaag gcccgatcac
     6961 ctggtcgaag cgcctcgtag atatagacga cgaccgcgtc actgttgagg tgctggcgga
     7021 aaacgtgggg ccggaggacg ccccgtgcac ggtccggttc ttctgctccg ctctggctgg
     7081 ccggcgcgac tcccccatcg agctcgtggt tgaaggcggg ccttccggcg caacccttga
     7141 aaccggcctc ggcatcgtac ggatgcctca ggagaccttc gcgctggaca ccgcctccgg
     7201 cgtgatgggc agctggggcg ttcagggcac cgacatcgga tggattgggc tcggcgtggt
     7261 atttcccccg gaacggtatc gccgggcagt cgattctgac accgagcacc aggtgatcct
     7321 cgatgcgaga cccggcacgc cgctccagta ccacattcag ggagactggc tgcggggtcg
     7381 acgttttccg cggtcgccag tcctgagcaa ctggatggac gaccttcgga gaacggctcg
     7441 gctcgcgaac ctgaaatgac ccgccgccac cgggtcgatt cccggagcct ggctggggta
     7501 gtatgaagac aacgtttaga atggccgcct gccggtccaa ccagacggta aaggatatga
     7561 caatgaaact tcgcgcaatc ctgctgacaa ccgtggcctt cgtggcgctt accagctacg
     7621 ccatggccca ggatcccatc gaggcgccac aatgggtcag cctgttcaac ggcaccgatc
     7681 tgaccgggtg gatcaatgtg aacaccgccg aggacacctg gtctgtgcga gatggactgc
     7741 tcgtgtgcaa aggacaccct atcggggtca tgcgcagcga aaaacagtat gagaatttta
     7801 ttctgcacat cgagtggcgg cacatggaac cgggcggcaa ctccggcgtg tttgtgtgga
     7861 gcgaaggcac cgtccctgag ggagcggagc tgcccaaagg cattgaggtg cagatgctcg
     7921 agctcgaatg ggtcaatttg aataaaaaac ccgacggcga actgcctccc atcgcctacg
     7981 tgcacggcga actgttcggt gcgaacggcg ccgtcaccat tccggacaac ccccgcggca
     8041 gccgcagcaa atcgatcgaa aaccgctgta aaggcaaggg tgaatggaat gtctacgacg
     8101 tagtgtgcgt ggatggcgtt atcaagctcg ctgtaaacgg caaattcgtc aacggcatca
     8161 gcggctcgtc ggtccggaag ggttacctgt gcctggaatc cgagggagct gaaatccact
     8221 tccgcaacat ccgcattctg gagctgccgc ccggcgtcat cactcctgag cagaccgcgc
     8281 cggtcgtgga accctagtcg cagagtcccg gcggcttagt cctcgtccga aaaccagtcg
     8341 ggcgggaggt acagcgacca gacgtcgttg cacagcggcg ggatcatgcc gcccgccacc
     8401 cagagtttgt cgtcatgcac aaacaacgag tgctcgtggc gttcccgcca gcgggcgccc
     8461 gctttcagag gcgtccaaac cgcaccgtct ttcgaatacc agacatcgtc ccagtttttt
     8521 gagggattgt tcgaccatcc gccgagcacc cacatgcggc cgcggtaaac ggccgacgag
     8581 aaccacagcc gggggtgcca cggggcggct tcggtgacgc aggtccaggt tttgccgtct
     8641 tcggacatcc acacgtcgtt aaacgcgctg taatccggca cataattacc tccgccgaac
     8701 acatagagct tgccatccag cacggccgcc tggtggtacg cgcgcggggg ccatcccgca
     8761 ttgtccgtca cctggaccca gtgctctccg tccgaggaac accacacatc gttcttcaag
     8821 ctcgaggcat cgccgaaata gtagttctcg gtccccccca gcagccacat cttcccctga
     8881 aactcgacca gcgctgccgc acaccgcggc gaccagcctg ccgcggccgt agcctgctgc
     8941 cagttcgccc cgtcaatgga ccaccatacc tcgttggagg ccgaatgccc cggcagacgc
     9001 ccgttgtacc atccgcccat gagccacatc cggtcgttga aagcgatcgt catcggcaaa
     9061 tcgctgtgaa tccagggcgc agtctccagc acgcgcgtcc aggtcttgcc atcgggcgag
     9121 ctccagacgt cgcgtggcgg cgcctcgtag gactgcagcc atccccccag gagccacagg
     9181 cgcccgtcgt agaccatctc ccctgccgaa tcgcgcggcg cccatgccgc ggcttcggtc
     9241 tcctgcaccc attcgggctg cgctttttcc gcgaatgcac tcgccccaaa gacaaaaaca
     9301 agcagaaaca cgccaacgac tctcatgttc gcccccatcg cgtttagagt acacgggttc
     9361 gggtctgtag gcctgattct agtggattga atcaggccat tgcacgctgc tgtaccgcac
     9421 gctggcggga ggcgtttgaa tccccgtgtg caactgggga tactggcgct gttactggtg
     9481 cgcatcaaaa ctgccatctg gccgcagtgc aaggacagtc aggatagtga cctgggtcgc
     9541 ctgatagctg ttgtctcgcc tgttgcccgg tgaaaaccaa atattttgac ttttgtcatg
     9601 cgagggcgcg tttcatgaaa tcacccgtct ttaccactgc aaaaccaggg atcctccatc
     9661 gtctgccgtt gctcgtgatg ctgctgctgg cgccagcagc gtctgccgac tccaccgaag
     9721 tctggactcg ctgggaacac cggctggtga gcgaccgtgc ttatgagaat ccctgcgtgg
     9781 atgtggaggt ccgggtcctt tacgagaatg ccgaaggaga aacacgccgc ggcatggggt
     9841 tctgggacgg agaggacgcc tttgtcatca ggcaggcctt tccccggcca ggagactggc
     9901 gctggtcgac cgaatgctcg gatccatcca atcagggact tcagcaaacg ggcacggtgc
     9961 gcgtggtgcc tggcagcgcc cggaatcctc tggttcgtca tggctacctc cgcgtttccg
    10021 acgacgcccg cacactgagc tatgccgacg gcacgccatt tctctgggtc ggcgatacgt
    10081 gctgggctgc accggcccac agcaccttcg aagaatgggc gcgttacctg gacagtcgtg
    10141 cggcgaaggg ctacacggtg ctgcagacgt ccatcgcacc ggagtgggca ttgaagcatt
    10201 cgcggcgagg cattccgccc tttttgtcaa gactgccgga catctcacag cccaatcccg
    10261 cattcttccg ggagctggac cgcatgttcg atatggccaa cgaccaggga ttcgtcgtga
    10321 tgctgtgcgg cctgatggag acgccctatg aatacccgcc tgccggcgat atcgcgatct
    10381 tcagccgcta tgtcgctgcg cgttatgccg cccatgccct gatcttttcc ccgagcttcg
    10441 acagcggcat ccatgaagct gagaccctgg cttctgcgct tgcgattcgt gaagccgccc
    10501 cggcgaatat ggtcacgatg cacatgggca ctggtgtggg cccgcatttt cacggcgcag
    10561 actggctcgc cttcgatat
//
LOCUS       DAOPBQ010000051        10499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6161_length_10499_cov_4.200881, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000051 DAOPBQ010000000
VERSION     DAOPBQ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10499
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6161_length_10499_cov_4.200881"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /gene="xylA"
                     /locus_tag="PKW60_02935"
     CDS             complement(<1..1174)
                     /gene="xylA"
                     /locus_tag="PKW60_02935"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HPJ98417.1"
                     /translation="MPEYFPEVSAPIAYEGPESQKALAFKHYNPKKKVLGKTMAEHLR
                     FSVCYWHTFKGAGSDPFGPGTMLRAYNDAPDPMAQAEATMHAAFEFFTKLGVGFWCFH
                     DHDIAPEGATLAESNKNIDAITALAKKLQRKTGVKLLWGTSNLFAHRRFMAGAATNPS
                     PEIYAYAASKVKKALEVTKELGGAGYVFWGGREGYDTLLNTDMKRELDHLGRFLAQAV
                     EYKEKIGFKGQFYIEPKPKEPTKHQYDFDAASCHAFLQKYGLVDQFKLNIEANHATLA
                     GHTFIHELEYAAANGILGSVDANRGDLLLGWDTDQFPTDVRECTLAMYVILRAGGLKT
                     GGLNFDAKVRRQSVDPIDLFHAHIGAMDAFARGLENAARLLASKKLENFIQKRYSGW"
     gene            1466..2041
                     /locus_tag="PKW60_02940"
     CDS             1466..2041
                     /locus_tag="PKW60_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98418.1"
                     /translation="MEEGFPVIGGYRIDLKALTGIAHHCDPRQCDNGLSCCSCYDVEL
                     SPLEARRVVDWLPEAAKFARRLHDKEGFIDPVERMPGARPSLAVDAAERCVFSYKAPN
                     GGRWCALHSAALAHSLAPEAVKPLSCALWPLAISDDEDPPTLTVQPEAARFTCNSPRS
                     GDGLDAGIAEIIERCFGPRFRRDVEACLARR"
     gene            complement(2126..2641)
                     /locus_tag="PKW60_02945"
     CDS             complement(2126..2641)
                     /locus_tag="PKW60_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPJ98419.1"
                     /translation="MNTQHTFPIASTANRAQSALVDVLAIAGLMALGAWIRLPLPFTP
                     VPLTLQTLPVLVAAFLVGRDRAMAGALLYMALGLAGAPILAVGATFGVTFGYLTGFAA
                     APWVALRFRSPLAGLIAASAVILGAGAVWLSLYTGCSLGGAVRLGALPFVPGDALKAA
                     AAYAIIRSTRS"
     gene            complement(2739..3908)
                     /locus_tag="PKW60_02950"
     CDS             complement(2739..3908)
                     /locus_tag="PKW60_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HPJ98420.1"
                     /translation="MSETNDGTSAAHGGIRIDADYPGGNILVEGIEGDLVRLHPDLRD
                     TTTWWFYWNFRVRGAAGRTLRFVFSGQNPIGVRGPAVSTDGGASWAWLGTDTVDEGSF
                     WYAFPPGCDEVRFAFTYPYLEADLQRFLETCPGAGRIEKGELCVSRGGRSVEWLQLRP
                     RREKVRGRIVLLCRHHACESMASFMLEGVLQAAVSNTRDGRWLRRNVAILAVPFMDKD
                     GVEQGDQGKNRAPHDHNRDYIEEGIYPEVRAVRELIPRWAQGALSIGLDLHCPWIRGG
                     SGKPSDNEQVFFVGGPNPETWARTQVLASLLEAEQRGPIVYSPRYDVPYGVEWNKSTG
                     TCGDWLGTLGGGPAAATIEFPYANAGGTAVTPEAARLFGRDLAHAFRRYLELRDS"
     gene            complement(3952..5634)
                     /locus_tag="PKW60_02955"
     CDS             complement(3952..5634)
                     /locus_tag="PKW60_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPJ98421.1"
                     /translation="MMRLSLVMLMLITLAGCATMEKGSQGPMQCWAVDPLVKVFRDAT
                     PDAGGVALAEVARGEHASFQVVVRTDAGITGLTAKASPFRRARSRDTLIPDSVRFVGY
                     VPVDRPTQKPSKDQLRTPPADYPDPLLEDSALDVAAGQAQPVWVTVAVPVDATPGIYR
                     GTLTLEGQTGDVVAAVEFPLTLRVYDVAVESTRLWVTEWYGLQWQHMAINPEPESEEY
                     YALLRRYANNMAEHRHNVALISPLGLAAWEANPDGSLKIDFSRFDRWVTIFQEEGVLG
                     RIEGGHIGGRVGGWESDFVVQIRRVQDGKVISESVAPGAPEADAFYAQFFPALVAHLK
                     EKGWLDIYMQHLADEPIASNVESYRAMAALARKYAPELLIMEACHTKELAGAMDIWVP
                     QLNFLHDDFEHYRERQAAGEEVWFYTCVFPQGEYANRFLEQPLIKTRLLHWINFRYGV
                     TGYLHWGYNQWTQDSPFTHSTRKHGGPPYLPAGDPWIVYPGKQGPLDSIRFEAMRDGI
                     VDHELLCRLAEIDPAAAETLAAKHIIDFDQYDTGVEAFRASRRELLERLAAH"
     gene            6002..7159
                     /locus_tag="PKW60_02960"
     CDS             6002..7159
                     /locus_tag="PKW60_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPJ98422.1"
                     /translation="MRRTFPIALGLCVAVAAVSFQAVAADAKPIRIGALFSITGPASF
                     LGAPEEKTVRMLADKINAEGGVLGRKVEVIIKDTQGSPEKAVSFARQLIEEERVLAII
                     GPSTTGETMQIKGLCEENEMILVSCAAAEAITNPVARYVFKTPQKDSQAVTWIYNTMK
                     DLGISKIAVVTSNDGFGNQGKLQLEALAPEHGIQIVVNEVYDKQATDLTDIMTKVKSA
                     AGVQAVVNWSIVPAQSIVAKNMKQIGLNVPLFQSHGFGNPKYVEQAGVAAEGTLFVAG
                     RLLVVDELPDDHPQKALLATYKKDYETRYNEPVSTFGGHAYDAFTVVLEGIRQAGTTD
                     RAKVRDAIENLKGFAGTAGIFNFSPEDHTGLSLDAFTMQTVKDGKFRLYSK"
     gene            7257..8150
                     /locus_tag="PKW60_02965"
     CDS             7257..8150
                     /locus_tag="PKW60_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPJ98423.1"
                     /translation="MGQSDLILQYFLSGITKGSIYAVVAIGFNLIYSATGILNFAQGE
                     FVMLGGMLAVSLALILPLPVAVAGAVVFVALLGGLLELVFFRRLRGHSILHLVIITIG
                     LSILIQEAALHVWDEKVRSLPYFTGNEISSIRLFGAAVSPQVLWVLGTVAIIVVFLHI
                     FLKYTLRGRAMRACSSNPEAALLAGINIANMRTLSFMLSAGLGALAGCVISPITLTHY
                     EMGSGLAIKGFSAAILGGLGNPMAAVLGGLLVGVLEAFSVSVLPAAYTDVTAFAILLL
                     VLFVRPHGLLGRPAGESVREF"
     gene            8152..9240
                     /locus_tag="PKW60_02970"
     CDS             8152..9240
                     /locus_tag="PKW60_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPJ98424.1"
                     /translation="MRLRTAIEIVALIVALVAAQLVLRALDKEYCLTQLTMAIYYAIV
                     VIGLCFIMGYAGQVSLGHGAFFAIGGYTTAVLTTSDFSRFEAAAWGRLLKHLRVLVVR
                     QDMYGADVLSAAPWAAFALAMAATLVIAALIGYPALRLKGHYLTMATLGFGLIVYKII
                     LGSTITGTTDGIVGVPAWSFGNFVAISGKSAGRVHNYYIASSIALLVLVLLRNLVHSR
                     VGRALRAIHDHETAANAMGINTARYKLKAFVASALLAALAGVLLTHYTGGVNPSEASA
                     MKSVRYVALATAGGSTSLWGAAAVSIVLNFLSLRGWFGSYDNAVFGALLITIVSLAPE
                     GPLKPLGQVLRRFRRRGGEDARSNDGAS"
     gene            9212..10015
                     /locus_tag="PKW60_02975"
     CDS             9212..10015
                     /locus_tag="PKW60_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98425.1"
                     /translation="MRGATMALLEVQNVRRAFGGVVAVDQVSFSVEAGQIKAIIGPNG
                     AGKTSLFNLISGLLKPESGRVRFKGREIGGRKPYQIAQAGLARTFQNPSLFLQISVLE
                     NVMVGQHCRTRSEFAGCSLRTPGQRREERAMRDSALSRLDFVGLAGHAAAPAGALAFG
                     QRRMVELARALATEPDLLLLDEPASGLNMRETDGLGQLIEKIRDTGITVLLVEHDMSL
                     VMDISDEILVLDNGATIAEGSPQAIQNDPNVIAVYLGSDFKHASTSQES"
     gene            9990..>10499
                     /locus_tag="PKW60_02980"
     CDS             9990..>10499
                     /locus_tag="PKW60_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98426.1"
                     /translation="MLLQVKNLRCGYGGLEVVHGISLHVCPGEIVGLLGANGAGKTTL
                     LKALVGLLRPWRGEILIHGRPVQNQAAWRCVSNAVALVPEGRQIFADMTVRENLLIGG
                     YHNAYRRMDVEMVCERFPRLAERINQLGGTLSGGEQQMLALGRALMARPKMLLLDEPS
                     MGLAPLMARE"
BASE COUNT         1890 a         3378 c         3275 g         1956 t
ORIGIN      
        1 cccaccctga ataccgtttc tggatgaaat tctcgagctt cttgctggcc agcagccggg
       61 ccgcgttctc gagaccgcgg gcgaaggcgt ccatcgcgcc gatatgcgcg tgaaacaggt
      121 cgatggggtc cacggactgc cgccgcacct tcgcatcaaa gttgagcccg ccggtcttga
      181 gtccgccggc ccgcaggatg acgtacatgg cgagggtgca ctcgcggacg tccgtcggga
      241 actggtccgt atcccagccg agcagcaggt cgccgcggtt ggcgtcgacc gagcccagga
      301 tgccgttggc cgcggcatat tcaagctcat gaatgaaggt gtggcccgca agcgtggcat
      361 ggttggcctc aatattgagc ttgaactggt ccacgaggcc gtatttctgc aaaaaggcgt
      421 ggcaggaggc ggcatcgaag tcgtattggt gtttggtggg ctctttgggc ttgggttcga
      481 tgtaaaactg gcctttgaac ccgatttttt ccttgtattc gaccgcctgg gccagaaaac
      541 ggccgagatg gtcgagttcg cgcttcatgt cggtattgag gagggtatcg tagccttcgc
      601 ggccgcccca gaacacataa cccgcgccgc cgagctcttt ggtgacctcg agggcctttt
      661 tcaccttgct cgccgcgtag gcatagattt cgggggacgg gttggtcgcg gcgccggcca
      721 tgaagcggcg gtgcgcaaaa aggttgctcg taccccacaa aagcttcacg ccggtcttgc
      781 gctggagttt tttcgccaac gcggtgatgg cgtcgatatt tttgttgctc tcggcgagcg
      841 tcgctccttc cggggcgatg tcgtggtcgt ggaagcacca gaatcccacg cccagtttgg
      901 tgaaaaactc aaaggcggcg tgcatcgtcg cctcggcctg ggccatcggg tcgggcgcgt
      961 cgttgtacgc ccgcagcatg gtgcccgggc caaacggatc gctgcccgcg cccttgaacg
     1021 tgtgccagta gcacaccgaa aaccggagat gctcggccat ggtcttgccg agcaccttct
     1081 ttttgggatt gtaatgcttg aacgcgaggg ccttctgcga ctccgggccc tcgtatgcga
     1141 tgggcgcgct cacttcgggg aaatattcgg gcatcggtct ctcctcccgc tcgccggacg
     1201 gtccgcccgg cgcgatagct tcgcatgcgt gtcccgccag cttagcacat gcgccaggcc
     1261 aggcaaaaca cggtaaaatt ccccggaagg tccgggtgga tcctcatgag ccacccaggg
     1321 cgcggccggc gccgccccgg gatatgcaaa aagatcacgc ttgtgattta tggaaatctg
     1381 tattataaac actacttaag aatcactttg gagaagcgcg ggggttcgcg ggtacttccc
     1441 tgacgccagc acgtgaggaa atcgcatgga agaaggtttt cctgtcatcg gcggttaccg
     1501 gatcgatctg aaagccctga ccggtatcgc ccaccactgc gaccccaggc aatgcgacaa
     1561 cgggctgagc tgctgctcgt gctacgacgt ggaattgtcg cccctggagg cccggcgcgt
     1621 ggtcgactgg cttcccgagg ccgccaagtt tgcccggaga ctacatgata aagagggctt
     1681 tatcgatccg gttgagcgca tgccgggcgc acggccctcg ctggccgtgg atgccgctga
     1741 acgctgcgtt ttttcttata aggcgcccaa cggcggccgg tggtgcgcgc tgcattccgc
     1801 cgcgctggcc catagcctgg cgccggaggc ggtcaaacca ctcagttgcg ccctgtggcc
     1861 gctggccatt tccgacgacg aggacccgcc caccctcacg gtccagcctg aagcggcccg
     1921 tttcacgtgt aactccccgc gatcggggga tggcctggat gccggcatcg ccgagatcat
     1981 cgagcgctgc ttcgggccaa gattccggcg cgatgtcgag gcttgcctcg cccggagata
     2041 agccgggccg ctgagtgatc gtcttagcgg gcgaaagacg ccgcaacgag tgcgctgcag
     2101 gccgggggga cttccggaga cctgttcagg aacgggtgct gcggataatc gcgtacgccg
     2161 cggccgcctt gagcgcatcg cccggcacaa acggcagcgc gcccaatcgc accgcgccgc
     2221 cgagcgaaca acccgtgtac aggctcagcc acacagcgcc agcgcccagg atcaccgctg
     2281 aggccgcgat cagccccgcc aggggactgc ggaagcgcag tgccacccac ggcgccgctg
     2341 cgaaacccgt cagatagccg aaagtcacgc caaacgtggc gccgaccgcc aggatcggtg
     2401 cgcccgccaa tcccagggcc atgtacagaa gcgcccccgc catcgcgcgg tcgcggccca
     2461 cgagaaaggc ggccaccagc acaggcaggg tctgcagcgt gagcggcacg ggcgtgaagg
     2521 gcagcggaag ccgaatccat gcgcccagcg ccatcaggcc cgcgatcgcg agcacgtcca
     2581 ccagcgcgga ttgcgctctg ttcgcagtag atgcgatagg aaatgtgtgt tgcgtgttca
     2641 tggggcgcga gtgtagcaga gcccgatcct ccggttcaac gaagcaaaac taaccgccca
     2701 gacagccggg agacacccgt tctgtgcccg gcgtgaactc agctgtcccg caactcaaga
     2761 taccgtcgaa aagcgtgtgc caggtcgcgc ccgaacagcc gcgcggcctc cggagtcacg
     2821 gcggtcccgc ccgcattggc gtacgggaac tcgatcgtgg ccgccgcggg cccgccgccg
     2881 agagtgccga gccagtcccc gcaggtgccg gtgcttttgt tccattccac cccatacggg
     2941 acgtcgtacc gtggcgaata gacaatcggg ccgcgctgtt cggcctccag cagcgacgcg
     3001 agcacctgcg tacgcgccca ggtctccgga ttggggccgc ccacgaagaa cacctgctcg
     3061 ttgtcgctcg gcttgcccga accgccgcga atccaggggc aatgcaggtc gaggccgatg
     3121 gacagcgcgc cttgcgccca gcgcgggatc agttcacgga cagcccgcac ctccgggtag
     3181 atgccctcct cgatgtagtc gcggttgtga tcgtgcggcg cacggttttt cccctggtcg
     3241 ccctgttcga cgccgtcttt gtccatgaac gggacggcga gtatcgccac gttgcggcgc
     3301 agccagcggc cgtcgcgcgt gttgctgacg gcggcttgca gcacgccttc gagcataaag
     3361 ctcgccatgc tttcgcaggc gtgatgccgg cagagcagca cgatgcggcc ccggactttt
     3421 tcgcgccgcg gcctgagttg cagccattca acactccgcc cgcctctcga cacacacaac
     3481 tcgccctttt ctatgcgtcc ggcgccgggg caggtctcga gaaagcgctg cagatcggct
     3541 tccaggtatg gataggtgaa cgcaaaccgg acttcatcgc agcccggcgg gaaggcgtac
     3601 cagaacgagc cctcgtcgac cgtatctgtg cccagccagg cccaagacgc tccgccatcc
     3661 gtgctgacgg ccggtccgcg cacgcctatg gggttctgcc cgctgaatac aaagcgtagg
     3721 gtgcgtcccg ccgcgccacg gacccggaaa ttccagtaga accaccaggt ggtggtgtcg
     3781 cgcaggtcgg gatgcagccg taccaggtcg ccctcgatgc cctcgacaag gatgttgccg
     3841 cccggatagt ccgcgtcgat ccggatcccg ccgtgcgctg cgcttgtgcc gtcattcgtt
     3901 tcactcatgt gagccctttc cgggcgacgt cctgcccgtg ccatgcgtcg atcagtgagc
     3961 cgccagccgt tcgaggagtt cgcggcgcga agcccggaag gcctcgaccc ccgtgtcgta
     4021 ctgatcgaaa tcgatgatat gtttcgcggc aagggtttcc gccgctgcgg gatcgatctc
     4081 cgcaagcctg cagagcaatt cgtggtccac gatcccatcg cgcatcgcct cgaagcggat
     4141 cgagtccagc gggccctgtt ttccgggata gacgatccac ggatcgcccg caggcaggta
     4201 gggcggcccg ccgtgcttgc gtgtggagtg cgtgaaagga ctgtcctggg tccactggtt
     4261 gtatccccag tgcaggtagc ccgtgacgcc gtagcggaag ttgatccagt gcaggagccg
     4321 cgttttgatc agcggctgtt cgaggaagcg gttggcgtat tcgccctggg gaaacacgca
     4381 ggtgtaaaac cacacttcct cgccggccgc ctgccgttcg cggtaatgct caaaatcgtc
     4441 atgcaggaaa ttgagctgcg gcacccagat gtccatcgcg cccgcaagtt ccttggtgtg
     4501 gcaggcctcc atgatcagca gttccggcgc atatttccgc gccagcgccg ccatggcccg
     4561 gtaggactcc acgttgctgg caatgggttc gtcggccagg tgctgcatgt agatgtcgag
     4621 ccagcccttc tctttcagat gcgcgaccag cgcggggaag aactgcgcgt aaaacgcgtc
     4681 cgcttccgga gcgccgggcg ccacgctttc gctgatcacc ttgccgtcct gcacgcgccg
     4741 gatctgtacg acgaagtctg actcccagcc gccaacgcgc ccgccaatat gccctccttc
     4801 tatgcgcccg agcacgccct cctcctgaaa gatcgtgacc cagcggtcga agcgcgagaa
     4861 atcgatcttg agcgagccgt ccgggttggc ttcccaggcc gcgagcccga gcggggaaat
     4921 caaggcgaca ttgtggcggt gttcggccat attgttcgca tagcggcgta acagcgcgta
     4981 gtattcttcc gactccggct ccggattgat tgccatgtgc tgccactgga ggccgtacca
     5041 ctcggtcacc cagagccgcg tcgattcgac ggccacgtca tacacacgca gcgtcaacgg
     5101 gaattccacg gccgcgacca cgtcccccgt ctgtccttcc agggtcagcg tgcctcgata
     5161 gatcccgggt gtcgcatcga cgggcaccgc aaccgtcacc cagaccggct gggcctgccc
     5221 ggcggccaca tccagggcgc tgtcctcgag caaaggatcg ggatagtccg ccggaggtgt
     5281 tcgcagttga tcttttgacg gtttctgggt ggggcgatcc acggggacgt atcccacgaa
     5341 gcgcacggag tccgggatga gcgtgtctct gctacgcgcg cgcctgaacg gcgaggcctt
     5401 ggccgtcagg cccgtgatgc cggcgtccgt gcgcaccacg acctggaacg acgcatgttc
     5461 gccccgggcc acttcggcca gcgcaacacc gcccgcgtca ggtgttgcat cgcgaaacac
     5521 cttcaccagc ggatccaccg cccagcattg catggggccc tggctgcctt tttccatcgt
     5581 cgcacacccc gccaacgtta tcagcataag catcaccaac gacaaccgca tcatggtttc
     5641 ttacctttcc gagcattgcg cataccgccg caccggccgt ttcgtttgca acatattaga
     5701 aacctccgcc cgccaacgca accgcgcgcc ccgcgcccgg atcctgccgc aacagatgtc
     5761 cacccggccc acgtctgtcc acaaggtccg ctctgtccac gcgatccacg tgctcgtgcc
     5821 ctgaggctct cgccagcccg ccattgtcca cccccggcca cgcatggatg gcgcgattca
     5881 tacagttgac accatgccag cattctggca agctgacagc ttcactccaa ggctcggcaa
     5941 agcggtttat gagggggttc agtcgaccgg catcagcggt tctgcatcag ggaggcctat
     6001 catgagaagg acatttccta ttgcattggg gttgtgtgtg gcggttgcgg cagtgtcttt
     6061 ccaggcggtg gccgcggacg ccaaacctat ccggatcggc gcgctttttt ccatcacggg
     6121 cccggcctcg tttctgggag cgcctgagga aaagactgtc cggatgctcg cggacaagat
     6181 caacgcagag ggcggcgttc tcggacgcaa ggtcgaggtg atcatcaaag acacccaggg
     6241 aagtccagag aaagcggtct cgtttgccag gcaattgatc gaagaagagc gggtcctggc
     6301 cattatcgga ccctcgacca cgggcgaaac gatgcaaatc aaaggcctgt gtgaagaaaa
     6361 cgagatgatc ctggtctcgt gcgccgccgc cgaagccatt acaaaccccg tggcccggta
     6421 cgtcttcaag actccgcaaa aagacagcca ggcggtcacg tggatttaca acacgatgaa
     6481 agacctcggg ataagcaaga tcgcggtggt caccagcaat gacggattcg gcaaccaggg
     6541 caagctgcag ctcgaagccc ttgcccccga acacggcatt cagattgtgg tcaacgaggt
     6601 gtatgacaag caggcgaccg acctcaccga tatcatgacc aaggtcaaga gcgctgcagg
     6661 cgttcaggcc gtggtcaact ggtccatcgt gccggcccag tctatcgtgg ccaaaaatat
     6721 gaaacagatt ggcctcaacg tgccgctgtt ccagagccat ggcttcggca acccgaaata
     6781 cgtggaacag gcgggcgtgg ccgccgaagg caccctgttc gtggcgggcc gcctccttgt
     6841 ggtagacgag ctgcccgacg accacccgca gaaagctctg ctcgccacct acaaaaaaga
     6901 ttacgaaacc aggtacaacg agcccgtcag caccttcggc ggacatgcct acgacgcctt
     6961 cacagtcgtc ctcgaaggta tccggcaggc cggtacgacc gatcgcgcca aggtccgtga
     7021 cgccatcgaa aacctgaagg ggttcgcggg cacggcaggg atcttcaact tttcgccgga
     7081 agatcacacc gggttgagcc tcgacgcgtt cacgatgcag acggtcaagg acggcaagtt
     7141 ccgcctgtat tcgaagtagc cgcgggccgg gcgggttccg gatgacccca gaggggggaa
     7201 ggagagacac gtcccggcag ggggaaagcc ggggcgttgt gccgcacggg aacaccatgg
     7261 gacagagcga tctcatactg cagtattttc tgtctggcat caccaaaggc agcatttatg
     7321 cggttgtggc gatcgggttt aacctgatct acagcgcgac cggcatcctc aattttgccc
     7381 agggcgaatt cgtgatgctg ggcgggatgc tggcggtcag cctggcgctc atcctgcctt
     7441 tgccggtggc ggtcgccggc gccgtggtgt tcgtggccct gctgggcggc ctccttgaac
     7501 tggtgttttt ccgccggttg cgcgggcatt ccatactgca cctggtcatc atcacgatcg
     7561 ggctgtcgat cctgatccag gaagccgcgt tgcacgtatg ggacgaaaaa gtccgctctc
     7621 tcccgtattt tacgggcaat gaaatctcct cgatccgcct gttcggggcc gcagtctcgc
     7681 cgcaggtgct ctgggtgctg gggaccgtgg ccattattgt ggtgttcctg cacatcttcc
     7741 tgaaatacac cctgcgcggc cgcgcgatgc gagcctgttc ctcaaatccg gaggcggccc
     7801 tgcttgccgg gatcaatatc gccaacatgc ggaccttgtc gttcatgctg agcgccggat
     7861 tgggcgccct ggccggctgc gtgatttcgc ccatcaccct gacccactat gaaatgggca
     7921 gcggcctggc gatcaaaggg ttttccgcgg ccatcctggg cgggttgggc aatccgatgg
     7981 ccgcggtgct gggtgggctc ctggtcggcg tgctggaggc cttcagcgtg agcgttctgc
     8041 ccgccgcata caccgacgtg accgcgttcg ccatcctcct tcttgtgttg tttgtgcgcc
     8101 cgcatggcct tctgggcagg cccgcgggcg aaagcgtcag ggaattttag cgtgagactg
     8161 aggacggcca tcgaaatcgt cgcgctcatc gtcgcgctgg tcgcggcgca gctcgtcctg
     8221 cgcgctttgg ataaggaata ttgcctgacg cagcttacca tggccattta ctacgcgatc
     8281 gtcgtcatcg ggctctgttt catcatgggc tatgccgggc aggtttcgct gggccacggc
     8341 gcgtttttcg caatcggagg gtataccacg gcggttctga ccacctccga cttctcccgc
     8401 ttcgaagcgg ccgcctgggg acgtctgctg aaacacctcc gagtgctcgt cgtgcggcag
     8461 gatatgtacg gcgccgacgt gctcagcgcg gccccctggg cggcttttgc gctcgccatg
     8521 gctgccacgc tcgtgattgc cgccttgatc ggctatccag cgctgcgcct caaagggcac
     8581 tacctgacga tggcgacgct cggtttcggc ctgatcgtgt acaagatcat cctgggcagc
     8641 acgattaccg gcacaacgga cgggatcgtc ggcgttcccg cgtggtcatt tggaaacttc
     8701 gtggccatct cgggcaagag cgctggccgc gtccacaact actacatcgc gagcagcatc
     8761 gccctgctcg tgctggttct gctgcgcaat ctggtgcatt cccgtgtggg ccgcgcattg
     8821 cgcgccattc acgatcacga aaccgccgcg aacgccatgg gcatcaacac cgcgcgctac
     8881 aagctgaagg ccttcgtggc cagcgcgctg ctggccgcgc tggccggcgt gctgctgacg
     8941 cactacaccg gcggcgtcaa tccgtccgaa gccagcgcga tgaaatccgt gcggtatgtc
     9001 gcgctcgcca cggccggcgg ctccaccagc ctctggggcg ccgccgcggt aagcatcgtc
     9061 ctcaacttcc tctcgctgcg cggctggttc ggctcctacg acaacgccgt gttcggcgcc
     9121 cttctcatta ccatcgtctc cctggcgccg gagggaccgc tcaaaccgct ggggcaagtg
     9181 ctccgccgtt tccggagacg tggaggcgag gatgcgagga gcaacgatgg cgcttcttga
     9241 agtccaaaac gtacggcgtg ccttcggcgg cgtggtggcg gtggaccagg tctcgttcag
     9301 cgtcgaagcc ggccagatta aggccatcat cgggcccaac ggcgccggaa agacctcgct
     9361 gttcaatctc atctccggct tgcttaaacc cgaatccggg cgcgtgcgtt tcaagggacg
     9421 ggaaatcggc ggacgcaaac cctaccagat cgcgcaggca ggcctggcac gcacgtttca
     9481 gaatcccagc ctttttctcc agatcagcgt gctcgaaaac gtcatggtcg ggcagcactg
     9541 ccgcacgcga agcgagtttg ccggctgttc cctgcgaacg ccggggcagc gccgggagga
     9601 acgagccatg cgcgactcag ccctgtcgcg gctcgacttc gtcggcctgg ccggccatgc
     9661 cgccgcaccc gccggcgcgc ttgcgttcgg gcagcggcgg atggtcgagc tggcccgggc
     9721 cctggccacg gagcccgacc tcctcctgct ggatgagccg gcgagcggac tcaacatgcg
     9781 cgaaaccgac ggactcggcc agctcattga aaaaatacgc gacaccggaa tcacggtcct
     9841 tctcgtagaa cacgacatgt cgctggtaat ggatatttcg gacgaaatcc tggtgctgga
     9901 caacggcgcc acgatcgccg agggttcgcc tcaagccatc cagaatgatc ccaacgtgat
     9961 cgcagtttat ttgggcagcg atttcaaaca tgcttctaca agtcaagaat cttagatgcg
    10021 gttacggcgg ccttgaagtg gtgcacggca tcagcctgca tgtgtgcccg ggcgagatcg
    10081 tcggcctgct gggcgcaaac ggcgctggaa aaaccaccct gctcaaagcc ctcgtgggcc
    10141 ttttgcggcc ctggcgcggc gagatcctga ttcacggccg ccccgtgcag aaccaggcgg
    10201 cctggcgctg cgtgagcaac gcggtcgcgc tcgtcccgga aggacgccag atcttcgctg
    10261 acatgaccgt gcgcgaaaac ctgctcattg gcggctacca caacgcgtac cggcgcatgg
    10321 acgtcgagat ggtctgcgaa cgctttccac ggctggccga gcgcatcaac cagctgggcg
    10381 gcacgctctc cggcggggaa cagcagatgc tcgcgctggg ccgcgccctg atggcgcgtc
    10441 cgaagatgct gctgctcgac gaaccctcca tggggcttgc tcccctgatg gcgcgggag
//
LOCUS       DAOPBQ010000052        10422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6206_length_10422_cov_3.739462, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000052 DAOPBQ010000000
VERSION     DAOPBQ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10422
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6206_length_10422_cov_3.739462"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1289
                     /locus_tag="PKW60_02985"
     CDS             132..1289
                     /locus_tag="PKW60_02985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPJ98427.1"
                     /translation="MQDVYVVSAVRTAVGKANKGSLTQCRPDEMAAVVVKEAIDRAGV
                     PAGRIQDIVLGCAFPEGSQGMNIARVAQARAGVPDSVPGMTINRFCSSGLEAQAIAGA
                     KLEAGLLDVAVAGGVESMSSIPMGGLKYEPNPALTRERPEVYIAMGHCGDNVAREFNI
                     PRKELDAWALRSHQRAVSAIDQGLFAEEIVSLDVATPAGSIRFDTDEGPRRDTSLEVL
                     SRLKPAFAMPGRGFCTAGNSSQTSDGAAALCLMNRRAIEEFGVKPLARLRGYAVAAGD
                     PRFLGPAQLEAIPKACEQAGIAVSELGLIENNEAFASQCLYVIREMGFDPEKVNVNGG
                     AIALGHPLGCTGAKLTVQLLHEMRRRGVTCGLVTMCIGGGMGAAGVFELCG"
     gene            1476..2231
                     /locus_tag="PKW60_02990"
     CDS             1476..2231
                     /locus_tag="PKW60_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPJ98428.1"
                     /translation="MSGHSKWSTIKRKKGALDAKRGKIFSRLAKEISIAAKMGGGDPD
                     MNPRLRTVLLAARAENMPKDNIDRAIKKGTGELPGVTYEEARYEGYGPSGVALILDVL
                     TDNKNRTVSEIRHLLTRYGGSMAETGAVVWNFEQKGTIAVERGSMTEEQIFDKAIEAG
                     AEDVDTDSDDVFVISTEPHDLHAVAAALEEMGMPAKSAELTMIPKTTVRVEGNAVSTV
                     LKLMEALEEHDDVQNVFANFDISDEAMAAALEE"
     gene            2231..2728
                     /gene="ruvC"
                     /locus_tag="PKW60_02995"
     CDS             2231..2728
                     /gene="ruvC"
                     /locus_tag="PKW60_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019909060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPJ98429.1"
                     /translation="MRVLGFDPGTATTGYGVVEGRGTRLHHVIHGVITTPAGMHFAER
                     LRILHSEAAALVATYAPDAVAIEKLYFARNVTTGLKVAEARGVLALAAAQSNRPIGEF
                     SPLEVKNAVVGYGKATKKQVQEMVKLLLNLDALPRPDDAADALALAICQIHAGRLDSA
                     VRLRA"
     gene            2818..4242
                     /locus_tag="PKW60_03000"
     CDS             2818..4242
                     /locus_tag="PKW60_03000"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HPJ98430.1"
                     /translation="MPSQQSPFVHKALACPACSEKTEHRFFRRRVYVVEESEPDQHAL
                     RYRWASDQVQRVNPLYYALFFCPHCFYADVSEDFAKPPETESLTATLHAFKKAGRDDV
                     RLQLVGRHIDYEDIDFESALRLHFLAVLIHSLVDKSQQDAYKLGRLYLRIAWLYREHM
                     QGLPAGLEDATGADSPPENIPPACDSGSAPLLLAAVDAAQEALKQFEPHRRTLERLAG
                     QRADELSRASHGNPYLSYLALLISASEQLGQDLHALRELAFRDVSGLLHGEGENAASS
                     PAQDETPRQEEARSRRRAHFLPSAVFMESLAALWDGVPRNEQEALVLALEAFRRALTH
                     DVRLENPQRRLKVSTLVAELAMRQGDFRGALEVIRSLHQLAMATRQKLQARLQEEELI
                     DVDAQHIENRIHRATATLESAADLRETVIQRMVQAELPRIRHAFAALPARARLKDFEA
                     ALARQGIEKEVITYLKKRNMQLPT"
     gene            4334..4879
                     /locus_tag="PKW60_03005"
     CDS             4334..4879
                     /locus_tag="PKW60_03005"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPJ98431.1"
                     /translation="MRELPAGFDLNIVLIGMPGVGKSTVGVLLAKALSRNFFDTDLHI
                     QSYECCRLQEILDGRGRDIFLTLEERYVLTLELQGHVIATGGSVVYSTHAMEHLAADG
                     VVVHLDLPFDLLERRINNLAIRGVVMGAGQTLRTLYDEREPLYRRYADLSVPCAGLGH
                     DEAVLAIIEQLRGAGFSVGSD"
     gene            complement(4982..5776)
                     /locus_tag="PKW60_03010"
     CDS             complement(4982..5776)
                     /locus_tag="PKW60_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPJ98432.1"
                     /translation="MSKDDDCSGSGIAERQLGTGNREDEECRAGARRSQDVDTLPVEG
                     LGWYSRGYLPHADFPGEIQSITYHLADSVPADALAAIEDDLRSVVRDRRDAERRRKLE
                     EWFDAGYGSCVLHDPRAAACVLGNWTHFAGERYDLIAWVVMPNHVHVMVRVYEGIQLK
                     LIVQSWKSYTGRRIRQWMPGIAERQLGTGNREDEECRAGARRSQENRSRVWHRDCWDR
                     YIRDDRHFAAVVDYIHQNPVKARLVRRPEDWPYSSAAEWHYEQDER"
     gene            complement(5796..8060)
                     /locus_tag="PKW60_03015"
     CDS             complement(5796..8060)
                     /locus_tag="PKW60_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HPJ98433.1"
                     /translation="MTHWHHNNQPGDTLPPDSGGQQESHLPGHTPPAPGDLDMVQGVL
                     EHIVYENADTGFFVARLRDSKTNEQVTFVGNLAAVSPGETIRIWGRWVNDARFGRQLR
                     MERYETILPSTVHGIEKYLGSGLIPGIGPEYAKRLVKAFGVDTLRVISEEPRRLQTVP
                     GIGQKRAEQLRAAWEKQRAVRSIMLFLQGHGIGASQAIRIYKQYGDKAAAILRENPYR
                     LAQDIAGIAFKSADKIAEQLGVARNAPQRIEASMAYLLDRAGDSGHVFEQKDALIKDA
                     KELLDIEEDAGAFEAALASLESCGLVVRENDSIFLTKLWQAETECARLLKRLITAAAE
                     TVPIHTDKAIEWVEATQSIQLSDEQREAVRTAIHSKVLVITGGPGTGKTTVLNGILAI
                     FNAKNVSMELAAPTGRAAKRMEAATGREARTIHRLLEFSPKEGGFQRNEARKLEAGLF
                     VIDEASMVDITLMHSLLRAIPPEARLILVGDVDQLPSVGPGNVLLDVIASQAVPVVWL
                     KTVFRQASQSGIVANAHRINKGGFPEFNTTDFFFVERKDPGQAMETIVQLAAERIPAK
                     FGFDPKREVQVLAPMHRGEAGVTRLNEALQKALNPGGRRIERRPFGLGDKVMQLSNNY
                     ELDVFNGDTGIITLVDTELREVQITYDDRVVLYGFDDLDEVGLAYASTVHKAQGSEYP
                     AVVMPMLTEHFLLLQRNILYTGVTRASKIVVIVGDPKALGRAVRNIRPTQRNTRLADR
                     LANRAREHGGVSER"
     gene            complement(8266..9018)
                     /locus_tag="PKW60_03020"
     CDS             complement(8266..9018)
                     /locus_tag="PKW60_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPJ98434.1"
                     /translation="MMKHTTCAAMALLLSVLWLGGCATTMKARTTGDPGAPTGFINKS
                     MEVQGKERRYAVYVPRNYDSAKLWPLIVFLHGAGERGDDGLLQTEVGLPSAIRRHADE
                     FPAIVVMPQCPEGVWWDKAFEDIERAYELTRRQYSIDPRRIYLTGLSMGGYATWMYGA
                     QHSDRWAALLPICGGGNVEDAPALAKVPIWAFHGADDSVVKPEESRRMVEAVKNAGGT
                     VRYTEFEKTDHNSWDPAYRDKKTIKWLFKQSR"
     gene            complement(9341..>10422)
                     /locus_tag="PKW60_03025"
     CDS             complement(9341..>10422)
                     /locus_tag="PKW60_03025"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPJ98435.1"
                     /translation="PDELAAAQAEANVVMNEHWSVVDGALKYDGKGTSLCTAQDYADF
                     ELIVDWKIPAKGDSGIYLRGAPQVQIWDTAEWPEGSGGLYNNQNNPSKPLVCADNPIG
                     DWNTFYIKMVGERVTVYLNNALVVNKVIMENYWNREIPIYPSGQIELQHHGSDLWFTN
                     VYLREILPGEGWRPLFNGKDLTGWEQVGGKEQTWGAENGLLYTDAGDGGWLSTTEQFG
                     DFELSLEFRVPADGNSGVFCRTPREGNPAYEGFEVQVLDDYAEQYATLKPWQYTGSVY
                     GLAAPSRRVTLPAGTWQRMFIRCEGPKITVWVNGFQVTQTDMSQHQDKIQEHPGVART
                     SGYIGLQNHGSRLDYRNIQIRELNK"
BASE COUNT         1946 a         3222 c         3218 g         2036 t
ORIGIN      
        1 atgcaacata tgctggagac cgggaaaccc ctgcggaact aggcgcatgc gacttctaac
       61 cacgtcatag acatcgaaat tacctgcgcg ggcgcccacg ccggccccat gcagcgtcag
      121 gaggatgagg catgcaggat gtgtacgtcg tttcggccgt gcgcacggcc gtgggaaaag
      181 ccaacaaggg ctcgctgacc cagtgccgtc cggatgagat ggcggcggtc gtcgtgaaag
      241 aggccattga ccgcgcgggc gtgcccgccg gccgcattca ggacatcgtc ctcgggtgcg
      301 cgtttcccga aggcagccag gggatgaaca ttgcgcgggt ggctcaggca cgcgccggcg
      361 tacccgacag tgttccggga atgaccatta accggttctg ctcgtccggg ttggaggcgc
      421 aagccattgc aggcgcgaaa ctcgaagcgg gcctgcttga tgtcgcggtg gccgggggcg
      481 tcgagtccat gagttccatc cccatgggcg gcctgaaata tgagcccaat cccgcgctga
      541 cgcgcgagcg ccccgaggtc tatatcgcca tgggccattg cggcgacaac gtggcgcgcg
      601 aattcaacat tccccggaag gaactcgacg cctgggcgct tcgcagccat cagcgcgcgg
      661 tcagcgcgat cgatcagggc cttttcgccg aggagatcgt gtccctggac gtggctacgc
      721 ccgccggttc gatacgcttc gatacggatg aaggcccgcg ccgcgatacc tcgctcgagg
      781 tgctgtcccg tttgaaaccc gcattcgcca tgcccggccg cggattctgc acggcgggca
      841 attcgagcca gaccagcgac ggcgctgcgg ccctctgcct gatgaaccgg cgggcgatcg
      901 aggaattcgg ggtaaaaccg cttgcgcggc tgcgtggata cgccgtggct gcgggcgatc
      961 cgcgttttct cggcccggcc cagctcgaag ccatcccgaa ggcctgcgaa caggcgggga
     1021 tcgctgtgag cgagctcggc ctgatcgaaa acaacgaagc tttcgcttct caatgtctct
     1081 acgtcatccg tgaaatgggg tttgaccccg aaaaagtcaa tgtcaacggc ggcgcgatcg
     1141 cgctgggcca tcccctcggc tgcaccggcg ccaagctgac cgttcagctg ctgcacgaaa
     1201 tgaggcggcg cggcgtcacc tgcggcctcg tgaccatgtg catcgggggc ggcatgggcg
     1261 ctgccggcgt attcgagctc tgcggatagg cgttcggcaa ctcacggcgc agcgcccagg
     1321 attcatcgtc tgaaaaaggg ccggcgccgg cgcccgtgcg ctccgcattc gactggatgc
     1381 ggcgatttcc cgattgcggc aaaacgtggg ggaatgctat actcgacggt gcgctgcgag
     1441 cagtaatcac acagggccac gggagatacc gtacgatgtc cggtcattcc aaatggagca
     1501 cgattaaacg gaaaaagggc gctttggatg cgaaacgggg caagattttt tcgcgtctgg
     1561 ccaaggaaat cagcatagcg gccaaaatgg gcgggggcga ccctgatatg aacccgcgtt
     1621 tgcgcacggt cctgctggcg gcacgggccg agaacatgcc caaagacaat atcgaccgcg
     1681 ccatcaagaa gggcacggga gaattgccgg gggtgaccta cgaagaagcc cggtacgagg
     1741 ggtatggccc cagcggcgtg gcgctcatcc tcgacgtttt gaccgacaac aagaaccgta
     1801 ctgtgtcgga gatccggcac ctgctcaccc ggtacggagg atccatggcc gaaacggggg
     1861 ccgttgtatg gaattttgag cagaagggca ccattgccgt cgagcgcggg tccatgaccg
     1921 aagagcagat tttcgataaa gccattgaag cgggcgcgga agatgtcgat accgacagcg
     1981 atgacgtgtt tgtcatctct accgagccgc atgatctgca cgcagtagcc gcagccctcg
     2041 aagagatggg catgcccgcg aagagcgcag agctcacgat gatccccaag accacggttc
     2101 gggtggaagg caacgccgtg tcgaccgtgc tgaaactcat ggaagcgctc gaggaacacg
     2161 acgacgtgca gaacgtgttt gcgaattttg acatttcaga cgaggccatg gcggcggcgc
     2221 tcgaagagta atgcgcgtct taggcttcga ccccggcacc gcgaccaccg gctacggtgt
     2281 agttgaaggc cgggggacac gcctgcatca cgttatccac ggtgtgatta ctacccccgc
     2341 cgggatgcat ttcgcggaac ggctccgcat cctgcacagc gaagcggccg cattggtcgc
     2401 gacctacgcg cccgacgccg tagccatcga aaaactgtat ttcgcgcgca atgtgaccac
     2461 cggcctcaag gtggcggaag cgcggggcgt actcgcgctt gccgcggcgc aatccaaccg
     2521 ccctatcggg gaattttctc cattggaagt gaaaaatgcc gtggtcggct atggcaaagc
     2581 caccaagaaa caggtgcagg aaatggtgaa gctgctcctg aacctggacg ccctgccccg
     2641 gccggatgat gccgcggatg ctctggccct ggccatttgc cagattcatg ccgggcgcct
     2701 ggacagcgcg gtgcgcctcc gggcctgact ggtctttcga ggccgaaaag agtaggcttg
     2761 attcaggcga gtctcgtggg gagggttgcc ggcggtttgc tcgattcgag atcggccatg
     2821 ccatcacaac aatcgccgtt tgttcacaaa gcgcttgcat gcccggcctg ctccgaaaag
     2881 accgagcatc gtttcttccg ccggcgcgtc tacgtcgttg aggaatcgga gccggaccag
     2941 cacgcgctgc gctaccgctg ggccagcgac caggtgcagc gggtaaaccc cctgtattac
     3001 gccctctttt tctgtccgca ttgcttttac gccgacgtgt ctgaagattt cgccaagccg
     3061 cccgaaacgg aatcgctcac ggcgacactg cacgcgttca aaaaggccgg gcgggatgat
     3121 gtccggctcc agttggttgg acggcacatc gactacgagg acatcgattt tgaatcggcc
     3181 ttgcgcctgc atttcctggc ggtgctgatt cactcgctgg tcgacaagag ccagcaggac
     3241 gcgtacaagc tggggcggct ctatctccgc atcgcctggc tgtatcgcga acacatgcag
     3301 ggtttgccag cggggctcga ggacgcgacc ggagcggact ccccgccgga aaacatcccg
     3361 ccggcctgtg actccggcag cgctccgctt ctgctggccg cggtcgatgc ggcacaggag
     3421 gcgctgaagc agtttgagcc gcaccggcgg acgctcgagc gcctggccgg acaacgcgcg
     3481 gacgagttgt cccgcgcctc tcatggcaat ccgtacctgt cttatcttgc gctcttgata
     3541 tccgccagcg agcagctcgg acaggacctg cacgcgttgc gtgagctcgc gtttcgtgat
     3601 gtatcgggcc tgctgcacgg cgaaggtgag aacgccgcgt ccagccccgc gcaggatgaa
     3661 accccgaggc aggaggaagc gcgttcacgc cggcgagccc actttctccc gtctgccgtc
     3721 ttcatggaga gtctggccgc gctctgggac ggcgttcccc gcaatgagca ggaagcattg
     3781 gtgctggcac tcgaagcatt tcgacgggca ttgacccatg atgtgcgcct tgaaaacccc
     3841 cagagacggc tcaaggtttc gacgctggtg gccgaactgg cgatgcgcca gggcgatttc
     3901 cgcggcgccc tcgaggtcat ccgcagcctg caccagcttg cgatggccac acgccagaag
     3961 cttcaggccc gcttgcagga ggaagaactg atcgacgtgg atgcgcagca tattgagaac
     4021 cggatacaca gagcgaccgc caccctcgaa agtgccgcag acctgcgcga gaccgttatt
     4081 caacgcatgg tgcaggccga gctgccgcgg atccggcacg ccttcgccgc gctccccgcc
     4141 cgcgccaggc tcaaggattt cgaggcagcc ctcgcccggc agggtatcga gaaagaagtc
     4201 atcacctatc tcaaaaaacg caatatgcag ttacccacgt gaagcgttgg ttcaaaactg
     4261 ccccgaaagg gcatagcatc ccacaatcca ctgcctgttt tggagaatag tgccaggaat
     4321 acggagacta tgaatgcgtg agcttcccgc cggtttcgac ctcaatattg tgctcattgg
     4381 gatgccgggc gtcggcaaga gcaccgtcgg cgtgcttctt gccaaggcgc tctcccgcaa
     4441 ttttttcgat accgacctgc atatccagtc ctatgaatgc tgccgtctgc aggagatcct
     4501 cgatggccgc ggcagagaca tctttctgac gctcgaggag cggtatgtgc tgactctgga
     4561 acttcagggc catgtgatcg cgacgggcgg cagtgtggtc tacagcacgc acgccatgga
     4621 gcatttggcg gctgatggcg tcgtcgtgca tctcgacctt ccgttcgatc tgctggaacg
     4681 gcgcatcaac aacctggcca tccgcggagt ggtcatgggg gccgggcaga cgctgcgcac
     4741 cctgtacgac gagcgcgaac ccctttatcg ccgttacgcc gacctcagtg tcccctgtgc
     4801 cggattgggc catgacgagg cggttctggc gattatcgag cagcttcgcg gcgccgggtt
     4861 ttccgtgggc agtgattagg aggacgatga cagcgcgatt ggcagaacga aattggtgcc
     4921 gtccgaaaac caagggggcg cgccgccggg atcgccgagc gccggcccgg catccttctc
     4981 atcatcgttc gtcctgctca taatgccatt cggccgcact gctatagggc caatcctcgg
     5041 gacgccggac cagcctcgcc ttcactggat tctgatggat gtaatcaacg accgccgcaa
     5101 aatgccggtc gtcgcgaatg taccggtccc aacaatctct gtgccacacg cggctacggt
     5161 tttcctggga tcgccgagcg ccagctctgc actcttcgtc ttctctgttg cccgtgccga
     5221 gctggcgctc ggcgatccca ggcatccact gtcgaatcct cctgcccgtg tagctcttcc
     5281 aggattgcac aatgagtttc agctgaatac cttcgtacac gcggaccatc acatgcacgt
     5341 ggttgggcat cacgacccac gcaatcaggt cgtatcgctc tcccgcgaag tgcgtccaat
     5401 tgcccagaac acatgcggca gctcgcggat catggagcac acatgagcca tacccggcat
     5461 cgaaccactc ctccagtttt cggcgcctct cggcgtcgcg gcgatcacgg acgacggaac
     5521 gcaagtcatc ttcaatcgcc gccaaggcgt cggctggcac actatccgcg agatgatagg
     5581 taattgactg tatctcgcca ggaaagtcag cgtgaggaag gtacccccgg ctgtaccatc
     5641 ccagcccttc gaccggtaaa gtatcgacat cctgggatcg ccgagcgcca gctcggcatt
     5701 cttcgtcttc tctgttgccc gtgccgagct ggcgctcggc gatcccagat ccagaacaat
     5761 cgtcatcttt cgacatagcc ggggcctccg tgaactcagc gttcagaaac gcccccgtgt
     5821 tctcgcgccc ggttggcgag ccggtcggcg aggcgagtgt tgcgctgggt ggggcggata
     5881 ttgcgcacgg cgcgcccgag agctttgggg tcgccgacga taaccacgat cttgctggcg
     5941 cgcgtgacgc cggtgtacag aatgttgcgc tggagcaaga ggaaatgttc cgtcagcatc
     6001 ggcatcacga cggcagggta ttcgcttccc tgggccttgt gcacggtgct ggcgtaggcc
     6061 aggccgacct cgtccaggtc gtcgaacccg tagaggacga cccggtcgtc gtaggtgatc
     6121 tgcacttcac gtaattcggt gtcgacaaga gtgatgatac cggtgtctcc gttgaacacg
     6181 tcgagctcat aattgttgga gagctgcatc accttgtcgc ccaggccgaa ggggcggcgc
     6241 tcaatgcgtc tgccgccggg gttgagggcc ttctggagag cctcgttgag gcgggtgacg
     6301 ccggcctcgc cccggtgcat gggcgccagc acctgcacct cgcgtttcgg gtcaaagccg
     6361 aatttcgccg ggatgcgttc ggccgccagc tgaacgatgg tttccatggc ctggccgggg
     6421 tctttgcgct ccacgaagaa aaagtcggtg gtattgaatt cggggaatcc gcctttattg
     6481 atacgatggg cgttggcgac gatgccgctc tgagaggcct gccggaacac agtcttcagc
     6541 cacaccacgg gcaccgcctg gctggcaatg acgtcgagca atacgtttcc gggccccact
     6601 gagggaagct ggtcgacgtc gcccaccaga atcagccggg cttccggtgg aatggcccgg
     6661 agcaggctgt gcatgagcgt aatgtcgacc atcgacgcct cgtcgataac aaagagcccg
     6721 gcctcgagct ttcgggcctc attccgctga aatccgccct ctttgggact gaattcaagc
     6781 aggcggtgaa tggtacgggc ttcgcggccc gtggcggctt ccatacgttt cgcggcgcgg
     6841 ccggtgggcg cggcaagttc catggagacg tttttcgcgt taaaaatcgc gagaatgccg
     6901 ttcaaaacgg tcgtttttcc ggtgccgggg ccgccggtga tcacgagcac tttggagtgg
     6961 atggccgtgc gcactgcctc gcgctgttcg tcggacagct ggatcgactg ggtggcctca
     7021 acccattcaa tggctttgtc ggtgtggatc ggcacggttt cggcggcggc cgtgataagt
     7081 cgcttaagga ggcgggcgca ttccgtttca gcctgccaca acttcgtgag gaaaatggag
     7141 tcgttttctc gcacgacaag cccgcaggat tcgagggagg ccagagccgc ttcgaacgcc
     7201 ccggcatcct cttcgatatc gaggagctcc ttcgcgtcct tgatcagcgc atctttctgc
     7261 tcgaacacgt ggcccgagtc tcccgcccgg tcgagcagat atgccatgct cgcctcgatc
     7321 cgctgcggcg cattacgggc cacgcccagc tgttccgcga tcttgtcggc gcttttaaag
     7381 gcaatgccgg cgatatcctg ggccagacgg tacgggttct cgcggaggat ggccgcggct
     7441 ttatctccgt actgtttata gatacgaatg gcctggctgg cgccgatgcc gtggccctgc
     7501 agaaacagca tgatcgaccg cacagcccgt tgcttttccc aggccgcacg gagctgctcc
     7561 gcgcgcttct gtccaatacc cgggacggtc tgcaggcgcc tgggctcctc actgataacc
     7621 cgcaaggtat cgacgccgaa cgctttgacc agccgctttg catattcggg cccgataccg
     7681 ggaatcagtc ccgaacccag atatttttcg atgccgtgca ccgtgctggg cagaatggtc
     7741 tcgtaccgct ccatacggag ctgccggccg aagcgggcgt cattgaccca gcggccccag
     7801 atgcgaatgg tctcaccggg cgacactgcc gcgaggttgc ccacaaaggt gacctgctcg
     7861 tttgtctttg agtcgcggag acgtgccacg aagaaacccg tgtcggcgtt ttcgtagacg
     7921 atgtgctcga gcacgccttg aaccatgtcc aggtcgcccg gggccggcgg tgtatggccg
     7981 ggcaggtggg actcctgctg cccgccgcta tcgggcggga gcgtgtcgcc gggctggtta
     8041 ttgtggtgcc agtgcgtcat ggaagctcat tttgccaaca atggccgggc ctgtccatag
     8101 cagctcatta aaacatgagg cacagaagaa gacttcggga tcttcgctga tggatgggat
     8161 ggtgtgacgg cgcaccggtg gtctcgagct ggtctggccg tgcaaggctg agcacggaac
     8221 aaccgacgcc agcagccagg atgcggcctg gcatcccgcc ggggcttacc tcgactgctt
     8281 aaagagccat ttgatggtct tcttgtcgcg gtaggcggga tcccacgagt tgtggtctgt
     8341 tttttcaaac tcggtgtagc ggacggttcc accggcgttc ttgacggctt cgaccattct
     8401 gcgcgattct tcgggcttga ccacgctgtc gtcggcgcca tggaacgccc agatcgggac
     8461 cttggcgagg gcgggggcgt cttccacatt gccgccgccg cagatcggca ggagtgccgc
     8521 ccagcgatca ctgtgctgcg cgccgtacat ccaggtcgca tagccgccca tcgacaagcc
     8581 ggtcaggtag atgcgccggg gatcgatgga gtactgcctg cgggtcagct cgtaggcgcg
     8641 ctcgatgtct tcaaacgcct tatcccacca cacaccttcc gggcactgcg gcatgaccac
     8701 aatggcggga aactcgtcgg catgacggcg aatcgcgctc ggaaggccga cttcggtctg
     8761 cagcaagccg tcgtcgccgc gctcgccggc cccgtgaaga aatacgatca ggggccacag
     8821 tttcgcggaa tcgtagttgc gcggaacgta gacggcatag cgccgctctt tcccctgaac
     8881 ctccatggat ttattgatga atccggtcgg ggccccgggg tctccggtgg ttcgagcctt
     8941 catggtagtc gcgcatcctc caagccacag aacacttaac aaaagcgcca tggccgcaca
     9001 ggtggtatgt ttcatcatgg tttgcccccg gtaaaaactg ctttaaccgg atgcaacgat
     9061 agcagaatga tggcccgtgg gcaatcgcac tccgtgcatt ttaattttgc gcgcatggct
     9121 tcaggctttg cctttcctgt tcgcgggtct tgcggctgaa ctggaaggag aaaaaggagt
     9181 ccaccccccc ggccacgcga agcgcgtggc cactcccgcc agcgggggac acaagacgca
     9241 gaactggcgg cactctcttc actctcccgg cctcctcaag caaaaaactc ccccggaccg
     9301 gcgtccgggg gagttattta accctcgtgc gtctgcaggg ctacttgtta agctcccgga
     9361 tctgaatgtt gcggtaatcg aggcgtgaac cgtgattctg caggccgatg tatccgctgg
     9421 ttctggcgac gcccggatgc tcctggattt tgtcctgatg ctggctcatg tccgtttgag
     9481 tcacctggaa cccgtttacc cagaccgtga ttttcggccc ttcacagcgg atgaacatgc
     9541 gctgccaggt accggccggc agcgtcacac gccgcgacgg cgcggcaagc ccgtagacgc
     9601 tgcccgtgta ctgccacggc ttgagcgttg catactgctc cgcgtaatca tccagcacct
     9661 gcacttcaaa cccttcgtag gccgggttgc cttcacgcgg tgtgcggcag aacaccccgc
     9721 tgttgccgtc tgccggcacc cggaactcca gcgacagctc aaaatcgcca aactgttccg
     9781 tcgtcgacag ccacccgccg tcaccagcgt cggtgtagag caggccgttc tcggcgcccc
     9841 aggtctgctc cttgcccccg acctgttccc agcccgtcaa gtccttccca ttgaaaagcg
     9901 gacgccatcc ttcacccggg aggatctccc gcagatagac gttggtgaac cagagatccg
     9961 aaccgtgatg ctgcagctcg atctggcccg acgggtaaat ggggatctcc cggttccaat
    10021 agttctccat gatgaccttg ttgaccacca gcgcgttgtt caaatacacc gtcacccgct
    10081 cacccaccat cttgatgtag aacgtgttcc agtcgccgat gggattgtcc gcgcaaacaa
    10141 gcggcttgct cgggttgttc tggttgttgt agaggccgcc cgatccttcc ggccattccg
    10201 cggtgtccca gatctggacc tggggtgcgc cgcgcaggta gatcccgctg tctcccttgg
    10261 ccgggatctt ccagtccacg atcagctcaa aatcggcgta atcctgggcc gtgcacaggc
    10321 tggtgccttt gccatcgtat ttcagcgcgc catccacgac gctccagtgt tcgttcatca
    10381 ctacgttggc ctcggcctgc gccgcggcca gctcgtcggg cg
//
LOCUS       DAOPBQ010000053        10419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6211_length_10419_cov_3.857198, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000053 DAOPBQ010000000
VERSION     DAOPBQ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10419
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6211_length_10419_cov_3.857198"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            940..2031
                     /locus_tag="PKW60_03030"
     CDS             940..2031
                     /locus_tag="PKW60_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPJ98436.1"
                     /translation="MISKNKGFTLIELLVVIAIIGILAAILLPALARAREAARRSSCQ
                     NNLKQWGLVFKMFANESKGEVWPYWSIDHTDTSFAGNKRMAAAVGAWQVYPEYVTDVM
                     IMACPSSGRYKDYAATDFSLARNTLGGCGQQTVDYATLNNEKDNPCYGKQAAPVSSTP
                     FDSGKPRFYNCDLNPNACAPYWHTDMTKGKFIDVRSYRYFGRLISQDWMTNTVDDYYC
                     VGAFIMGASGSHPGAIPTSFRDASSNIYWANRSNEFNVTLPSGKAITIRRLREGIERF
                     TITDINNPAGAAAAQSQIVVMYDESRAYGGGGGAAQAERFNHVPGGMNILFMDGHVEW
                     ARYHGHDAWPVNEFAYVYPAGITSGMDFP"
     assembly_gap    2755..2854
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3045..3344)
                     /locus_tag="PKW60_03035"
     CDS             complement(3045..3344)
                     /locus_tag="PKW60_03035"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HPJ98437.1"
                     /translation="MVGVNGIPGVPEPSTVRKAEAAAPVRQAPAGVPAADGVEISLEA
                     QAAARAVQAERLQAAGEDVRAERVAEAKRNLEQGAHRVQEVVKLVAARMTKQLVL"
     gene            3732..5309
                     /locus_tag="PKW60_03040"
     CDS             3732..5309
                     /locus_tag="PKW60_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPJ98438.1"
                     /translation="MRIANAWDCPGSSQFSQRVAQASCLRSSDGYTGAIRAGSHGGLP
                     LQSGRQPRGDGRPRPSLHFSGIGGTAMVAGARLALEAGCEVRGSDNVLYPPTSQMVEA
                     LGVPVATAYAAANLDWDPDCVILGNALSRGNPEVEAALSRKLHYLSLPEWLKNAVLRT
                     RRSVVIAGTHGKTTTAALTAFLLDATGRAPGFLIGGQPLNFPHSARLGAEGAPFVIEG
                     DEYDTAFFDKRAKFFHYLPEFAVVTSVEFDHADIYPDLESIIRAFRLMLRQIPREGRL
                     FVCADDLNALALREYAFCPVESYGFDASADWRGEIASFSGIPDGCVGLRVHHGGALWG
                     EIAVPMAGRHNLRNALAALAAGAALGVTAAEAARALPAFRGVKRRMEVFLESRDITFV
                     DDFAHHPTAIRETIAAARQRWPGRALHVLFEPRSNTVVTNRFQAEMNTAFREADAVWL
                     GPIHRAASIPESERLDRAAIVQALEEQGIRAAFSDDVAELVRGLEASAAPGTVVLILS
                     NGAFGGIYDRLRERYRA"
     gene            5437..6792
                     /locus_tag="PKW60_03045"
     CDS             5437..6792
                     /locus_tag="PKW60_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HPJ98439.1"
                     /translation="MNALDRYFGIRASGSTVQRELIGGLTTFATMSYIVFVQPAVLQV
                     AGMPSGSVLLATCLSSAAACLLMGLLARYPFALAPGMGENFFFSFTVVCTMGFSWQAG
                     LAIVFISGVLFLLLSLFSARDRLMRVLPDCLKNAIGPGIGLLIAFIGLQWGGIVTFNP
                     VTMVGLGSFRSAVPLMTAFGVLLTAALMALGVRIGILIGIVATTGIGLLTGVLSRPDT
                     AIAWSTETFFNLNFRELSMHWGRALTAIALLFFMDLFDTIGTLVGVGKQAGYIGDDGV
                     LPRAGRAFFSDAVGTCIGALFGTSTVTSFVESATGVAAGARTGLAAVVTGFCFVLAIL
                     LAPLVQIAGQDVGPAFYGVDAAGPHVAMYPAVAPALIVVGFLMMAPLRRIAWEDVTES
                     LPAFLTVAMMVFSYAIHEGIAIGCISFVAIKIATGRFKEVHPVLYGVAALLVLRYVFL
                     R"
     gene            complement(6821..7957)
                     /locus_tag="PKW60_03050"
     CDS             complement(6821..7957)
                     /locus_tag="PKW60_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98440.1"
                     /translation="MKSYNVGILGFGFIGKVHACGHLNIPLFYNPPPCTTRITHICTS
                     RMETAEAGRAQLGADVATTDYREVTENPDVQIVHICTPNHLHREQLLSAMAHNKHIYC
                     DKPLVAHLSEIEPLEEALTRYTGVAQMTFQNRFFPATLRARQLVEEGFLGRLLQFRAC
                     YLHAGSADPAAPLKWKLSAEAGGGVIADLASHVLDLLHPLVGDYAALTAATQIAYPDR
                     PSADDPAARLPVEAEDNVVLLARTRSGALGTIEATKLATGTEDELRFELHGSSGAIRF
                     NLMDPHHLEIYDATAPDKPLGGRRGWTRLDTGQRYAPPGGAFPSPKASIGWMRSHMAC
                     LANFLYCVAANRPAEPGLEQGIYIQRLIDAVRRSARSSSWVELD"
     gene            complement(8020..9291)
                     /locus_tag="PKW60_03055"
     CDS             complement(8020..9291)
                     /locus_tag="PKW60_03055"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPJ98441.1"
                     /translation="MSIVIKNGHVIDPASNLSAPRDVLIIGGRIARIDTDLSGSEVFD
                     ATDCIVCPGLVDIHVHFREPGFESKETIATGSQAAARGGFTSVVTMPNTEPPIDNGGM
                     VEFINRRARETACIKIWPAAAATKDRAGAEMTEMAELKAVGAVAVTDDGNDIASSWVL
                     RRCLEYADMCGLTYLGHCEDTSLIQNGAMNAGLNSTRLGLAGMPKAMEEIAVTRLVRL
                     AEMTGAKVHVCHVSSAGSVEIVRWAKARGIRVTCETAPHYFTLTDDAVTTFNTNAKMN
                     PPLREAEDVEAIKEGLRDGTIDCIATDHAPHTPAEKDVEFDYAPFGIVGLETSLGVVI
                     TELVEPGILTMEEAIRMMTWAPAQILDLPAGRLEQDGPADITIFAPKEEWAVHPEAFL
                     SRSRNTPFTGRVLRGQVKATLCDGRFVYREA"
     gene            complement(9303..10292)
                     /locus_tag="PKW60_03060"
     CDS             complement(9303..10292)
                     /locus_tag="PKW60_03060"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPJ98442.1"
                     /translation="MATLSESEQRVWTRKDLIGLDELSRDELEMILDTAPQFLAVSER
                     ESGIKKVPVLQGRLVVNWFYEPSTRTRTSFELAAKRLSADTLTMNASASSTKKGETLH
                     DTLYNIEAMHTDIVVIRHSAAGASRILAERHLSHIINAGDGAHEHPTQALLDAFTIRQ
                     HIRRAREDAQAGLDGLRVAIIGDIAHSRVARSNVWGLTKLGASVVLCGPRTLLPDAFT
                     KLGVDVTTDLREAIDGADVIYLLRLQLERQHKCLFPSIREYIRLFRIDNESLKAAKPD
                     ALVMHPGPINRDIELASEVADGPRNVILEQVTNGVAVRMAVMHLLVNKPRVAA"
BASE COUNT         1656 a         3133 c         3526 g         2004 t
ORIGIN      
        1 gaagcgcgcg gcccttgctg acggaggtat gcctgcaggc ccgagcgccc aataaaacac
       61 ggcgcgctgt cccccgctgg cgggggtgaa tcggcgccag ccgatttggg ggtggagttt
      121 tacggctgaa tcgccttttc cagagcctcg ttgcagcaat gttgtgttcg ttcccataaa
      181 ccttcgcccg caaaatctac ccccggccgc ggggacgccg cccggacaac atgacggcgg
      241 gcgcgcgggg atgggcggcc agcatctgga cccacaaaac agggcatttt ttggggaaaa
      301 ccctgccggg gggaggcctt gacatgcact gatacaggtg taataatagt ggcagcaaag
      361 gaactttacg gctttttggc agtatgtgcg gtagatgtgc cggagcagga tgggattcat
      421 cggcgggcac ataaggacac aaggttgtac agacccgttc gcagcatgcg gcgccatccg
      481 gttcggggcg cgggccgaag gtagggggcc agctgggaaa cgggctgacg gttcggagcg
      541 aacttctgac actggcgggt ggggtgcccc gaggtgcgtt tccggtagat gagcagcagg
      601 cagttgaggc tccgggcgct ctgcagggaa ttggccggcg gatgtggggg cagaccaatc
      661 ctggccaggg cgaatgaata ggacaccttg aatgctccac gtgtccgctg aggctgcggt
      721 gtatctccga tgcttggggg cggccgcggt ttccagaaag tgcagggtga ggagcacggt
      781 tatgggggca cgccggttct cggggcgcgt tgtcctcaaa aaaggaagaa aggagcaggt
      841 gcagagagct atacgtcagg tttcattgtg aatccctcat cttttgttat gaaggtttca
      901 gacgaggcgt ggcaacacgc aggaaaagga gtccctagaa tgatatcgaa aaacaagggc
      961 tttacgctca tcgaattgct ggtagtcatc gcgatcatcg gtatcctggc cgcgattctt
     1021 cttccggcat tggcccgggc ccgcgaagcg gcacggcgtt cgagctgtca gaacaacctc
     1081 aaacagtggg gtctggtctt caagatgttc gccaacgagt ccaagggcga agtctggccc
     1141 tattggagca tcgaccacac ggacaccagc ttcgccggca acaaacggat ggccgctgcg
     1201 gttggcgcct ggcaggtcta tccggaatac gtcactgacg tgatgatcat ggcctgcccg
     1261 tcgtccggcc ggtacaagga ctacgcggcg accgatttca gcctggcccg caacacgctc
     1321 ggcggctgcg gacagcagac cgtagactac gccaccctta acaacgaaaa agacaacccc
     1381 tgctacggca aacaggccgc gccggtcagc tcgaccccgt ttgacagtgg caaaccgcgc
     1441 ttctacaact gcgacctgaa tccgaatgcg tgcgctccgt actggcacac cgacatgacc
     1501 aagggcaagt tcatcgacgt gcgcagctac cgctatttcg gccgcctgat ctcgcaggac
     1561 tggatgacca acacggtcga cgactattac tgcgttggcg cgttcatcat gggcgcgagc
     1621 ggcagccacc cgggcgccat cccgacctcg ttccgcgatg cctcaagtaa catctattgg
     1681 gccaatcgga gcaacgagtt caacgtcacg ctgccgtcgg gtaaagcgat cacgatccgg
     1741 cgtctgcgtg agggcatcga gcgcttcacc atcaccgaca tcaacaaccc ggcgggcgcg
     1801 gctgcggccc agtcccaaat cgttgtgatg tacgacgaga gccgtgcgta tggtggcggc
     1861 ggcggcgccg cgcaggccga gcgcttcaac cacgtccccg gcggcatgaa catcctgttc
     1921 atggacggcc acgtggaatg ggcgcgttac cacgggcacg atgcctggcc ggtcaacgag
     1981 ttcgcgtatg tgtacccggc gggtatcacc tccggcatgg acttcccgtg agccaacgac
     2041 acagcgtgta gtgcgtagcc tcgcgtaagc gggctgcgtg aagtagagat gaagcgccgg
     2101 gagcagcgat gctcccggcg ctttctgttt tgtgtgtagc gcaggcatcc tgcctgccct
     2161 ggagcagtcc acccccggcc gcctgaggct tcccacgtac acccaggttt tcgcagtgcg
     2221 taggggcaga cccctgtgtc tgcccttctt taaggctgcc gcgctgggtc cacgcagcgc
     2281 acacgatcaa cgttttcgta ctcaatggca ctcgccagcc cattattgtc cacccccggc
     2341 cgcttcgcgg ccacccccgc cagcggggga cacaagacgc ggaaccggcg gcgcgtgcgc
     2401 tgtatcaagg tgaacgcgcg aggatgggcg gacgtgtggg gctatcccga tgggcggcca
     2461 cagggggccg cccctacacc ggacgcgcag acacatagcg atgggcggcc gcccgaggct
     2521 tccctgggca acccaggctt tcgcagcgcg tagcgcaggc atcttgcctg cgttcttctc
     2581 ttgcgtagca caggaccgcg tgcttgccct ggtccaccca ggttttcgca gcgcgtaggg
     2641 gctgaaccgt gtgccgacga ggacgtcggc gcacccaggg tacctatcct ccgcgggcag
     2701 ggctgaaccg tgtgccgacg aggacgtcgg cgcacccagg gtacctatcc tccgnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgggca ggggtgaatc ggcggcagcc
     2881 gattcggggg tggagtttta cggctggagc gcctttttcg gaggctggtt gcaaggccag
     2941 ggctatgtgc agacacagct gcagccccgg cgggcccgcc tctccgcgag tttgggcgcg
     3001 ccgtggctgt tactccagcc tgtccgttgt tcagcgtgcg gttcctacag tacgagctgc
     3061 ttcgtcatgc gagcggccac cagcttcacg acctcctgga cgcggtgggc gccttgttcg
     3121 aggttgcgtt ttgcttctgc gacccgctcg gcacggacgt cttcgcccgc tgcttgcagg
     3181 cgttccgcct gcactgcccg ggcagcggcc tgggcttcca ggctgatctc gacgccgtcg
     3241 gccgccggga cgcccgctgg ggcttgcctc actggcgcgg cggcctctgc cttgcgtaca
     3301 gtggaaggct cgggcacacc gggtatgcca ttcacgccaa ccattgccgt gctctccctg
     3361 gcattcgcgg tgaacctcca tgttcacctt ttcactcttt cttatcgaca gaatccgcgc
     3421 gggactttag cggggccgga ttgctccggc ggcgcggatg tgcttgaata gcggccggaa
     3481 tatgcatggt aaaacagggg gcgtctatga gcagtcagga tcaggacgga cgcgtggggt
     3541 tatcgggatg ggcggccaca gggggccgcc cctacaaccg gacgcggaga ggctatgcag
     3601 tccacccccg gccgcttcgc ggccaccccc gccagcgggg gacacaaggc gcggaattaa
     3661 ctgacgacgc ccgccctgaa gtcgtatcaa cgcgagccgt tagggcggtc gaccctgggg
     3721 gcgcccctac aatgcggatc gcgaacgcct gggactgccc tggatcatct caattttcgc
     3781 agcgcgtagc gcaggcatct tgcctgcgtt cttcagacgg atacacggga gctatccggg
     3841 cgggcagcca cggggggctg cccctgcaat cgggacggca accgcgcggg gatgggcggc
     3901 cacggccgtc gctccatttt tcgggcattg ggggtacggc gatggtcgcg ggggcgcgcc
     3961 tggcgctgga ggccggctgt gaggtgcgcg gcagcgataa cgtgctctat ccgcccacat
     4021 cgcagatggt ggaggcgctg ggcgtgccgg tagccacggc atacgccgcc gccaacctgg
     4081 actgggatcc ggactgcgtc atcctgggga acgcgctgag ccggggcaat ccggaggtgg
     4141 aagcggcgct ttcgcgcaaa ttgcactacc tcagcctgcc cgaatggctg aaaaacgccg
     4201 tgttgcgcac gcgccgctcc gtcgtcatcg cgggcacgca cggcaaaacc accaccgccg
     4261 cgctgaccgc ctttctgctg gatgccacgg gcagggcgcc gggctttctg atcggcgggc
     4321 agccgctcaa ttttccccat tcggcccggc tgggcgcgga gggggccccg tttgtcatcg
     4381 aaggcgacga gtacgacacg gcttttttcg ataaacgcgc gaaattcttc cattatctgc
     4441 cggaatttgc cgtggtgacc tcggttgaat ttgaccacgc ggacatttat cccgatctgg
     4501 agtcgattat ccgcgcgttc cggctcatgt tgcggcagat cccgcgcgag gggcggctgt
     4561 ttgtgtgtgc ggacgacctc aacgcgctgg cgctccggga atatgccttc tgcccggtgg
     4621 aatcgtacgg atttgatgca tcggccgact ggcgcggtga gatcgcgtcg ttttcgggca
     4681 ttcccgatgg ctgcgtgggc ctgcgggtgc atcatggggg cgcgctgtgg ggtgaaatcg
     4741 cggttccgat ggcgggccgg cacaacctgc gcaatgcgct ggcggccctg gctgccggcg
     4801 cggccctcgg cgtgaccgcg gcggaggccg cacgcgccct gccggcgttt cgcggggtca
     4861 aacggcgcat ggaggtgttt ctggaatcgc gggacatcac gtttgtcgat gatttcgctc
     4921 atcatcccac cgcgattcgc gagaccatcg ccgcggcgcg gcagcgctgg cccgggcgcg
     4981 cgctgcatgt gctgtttgag ccgcgctcga acacggtggt caccaatcgg ttccaggcgg
     5041 agatgaacac ggcgtttcgc gaagccgacg cggtctggct ggggcccata caccgcgcgg
     5101 cgtcgatccc cgaatcggaa cggcttgacc gcgcggcgat cgtgcaggcc ctggaggagc
     5161 agggcatccg cgcggcgttc agcgacgacg tggctgagct ggtgcgcggg ctggaggctt
     5221 cggccgcgcc gggcaccgtg gtgctcattc tcagcaacgg ggccttcggc ggcatctacg
     5281 accggctccg ggaacggtac cgcgcctgag gcagcggttg agcaggctgg cggggagagg
     5341 tacaatcgga tcgcacgacg ggcgcctgct gcgggccgcg tgagactgct ccggcgcgcc
     5401 ggatctcgtg ccaattccgt ggggatgggg gtgattatga atgctctcga ccgctatttc
     5461 ggcattcgcg cctcaggttc aacggtccag cgtgaactta tcggcggtct gaccacgttt
     5521 gccacgatga gttacatcgt gtttgtgcag cctgcggtgc ttcaggtggc cggtatgccg
     5581 agtggctcgg tgcttctggc gacctgcctg tcgtcggcgg cggcctgcct gctgatgggg
     5641 ctgcttgcac gctatccgtt cgcgctggcc ccgggcatgg gcgaaaactt ctttttctcg
     5701 ttcaccgtgg tgtgcacgat ggggttttcg tggcaggccg ggctggcgat cgtgttcatc
     5761 tcgggggtgc tgtttctgtt gttgtcgctg ttcagtgcgc gcgaccggtt gatgcgcgtc
     5821 ctgccggatt gcctcaaaaa cgcgattggc ccggggatcg gactgctgat cgcgttcatc
     5881 ggcctgcaat ggggcgggat cgtcacgttc aacccggtca cgatggttgg cctgggcagt
     5941 ttccggtcgg cggtgccgct catgaccgcg tttggggtgc tgctgacggc ggcgctgatg
     6001 gcgctggggg tgcgcatcgg cattctcatc ggcatcgtgg cgacgacggg catcggcctg
     6061 ctgaccgggg tgctgtcgcg gccggatacc gcgatcgcct ggtcgaccga gacctttttc
     6121 aacctgaatt tccgcgaatt gtcgatgcac tggggccgcg cgctcacggc catcgcgctg
     6181 ttgttcttca tggacttgtt cgacaccatc ggcacgctgg tgggcgtcgg gaaacaggcg
     6241 gggtacatcg gcgacgacgg ggtcctgcct cgcgcggggc gcgccttttt cagcgatgcc
     6301 gtgggcacct gcatcggggc gctgtttggg accagtaccg tcacgtcttt tgtggaaagc
     6361 gcgacgggcg tggcggcagg tgcgcgcacg ggtctcgcgg ccgtggtgac ggggttctgc
     6421 ttcgtgctgg ccattctcct ggcgccgctg gtgcagatcg ccgggcagga cgttggcccg
     6481 gcattttatg gcgtggatgc cgcggggccg cacgtggcca tgtatccggc ggtggcgccg
     6541 gcgctgatcg tcgtcggatt tttgatgatg gctccgctgc gccgcatcgc gtgggaggac
     6601 gtgacggaga gccttccggc atttctgacc gtggcgatga tggtcttttc atacgccatt
     6661 cacgagggga tcgccattgg ctgcatctct tttgtggcga tcaagatcgc aaccggccgg
     6721 tttaaggaag tgcatcccgt cctgtatgga gtcgcggcgc tgctggtgct gcgctacgtc
     6781 tttctgcgct aggcaggctt cctcccgtat ccatccggaa tcagtcgagc tcaacccagg
     6841 atgacgagcg cgcggaacgg cggacggcgt cgatgagcct ctggatgtag atgccctgct
     6901 cgaggccggg ttcagcgggc cggttcgcgg cgacgcaata gaggaaattg gcgaggcagg
     6961 ccatgtgcga gcgcatccaa ccgatgctgg ccttcgggct gggaaaggcg ccgccggggg
     7021 gtgcatagcg ctggcccgta tcgaggcgcg tccatccgcg gcgcccgccc aggggcttgt
     7081 ctggcgccgt ggcatcgtag atttcgagat ggtgcgggtc catgagattg aaccgaatcg
     7141 cgccactgga gccgtggagc tcgaagcgca attcgtcctc cgtgcctgtg gcgagcttgg
     7201 tggcctcgat ggtgcccagc gcaccggagc gtgtgcgcgc gagaagcacc acgttgtctt
     7261 cggcttctac cggcagacgc gccgccggat cgtccgcgga gggccggtcc gggtaggcga
     7321 tctgggtggc ggcggtcaag gcggcataat cgccgacgag cgggtggagc agatcgagca
     7381 cgtgagaggc caggtccgcg atcacgccgc cgcccgcctc ggccgacagc ttccatttga
     7441 gcggcgcggc ggggtcggcg cttccggcat ggaggtagca cgcgcgaaac tgcagcagtc
     7501 tgccgagaaa tccctcttcg acgagctggc gcgcccgcag cgtggccggg aaaaaccggt
     7561 tctggaaggt catctgggcg acgccggtgt agcgcgtgag ggcctcttcg agcggctcga
     7621 tctcgctcag atgggcgacg agcggcttgt cgcagtagat gtgtttgttg tgggccattg
     7681 cagagaggag ctgttcccgg tggagatggt tcggggtgca gatgtgcacg atctgcacgt
     7741 cgggattttc ggtgacctca cggtaatccg tggtagccac gtcggcgccg agttgtgcgc
     7801 ggcctgcttc ggccgtttcc atacggctcg tgcagatgtg ggtgatgcgc gtcgtgcagg
     7861 gcggcgggtt gtagaacaag gggatgttga ggtggccgca ggcatgcacc ttgccgataa
     7921 acccaaagcc cagaatgccc acgttatacg atttcatgcg gccccctcgt tctgccgggc
     7981 gattgcgcgg atcaggaacg accgtgcagc ggtgattgat caggcttcgc gatagacgaa
     8041 ccgtccgtcg cacagagtcg ccttcacctg cccgcgcagc acccggcccg taaacggcgt
     8101 gttgcggctt ctcgatagaa atgcctcggg atggactgcc cattcctctt ttggcgcaaa
     8161 aatggtgatg tctgcgggcc cgtcctgctc cagtcggccc gccggcaggt caaggatctg
     8221 tgcgggcgcc caggtcatca tccggatggc ctcttccatc gtgaggatgc cgggctcgac
     8281 cagctccgtg atgaccacgc cgagcgaggt ctcgaggccg acgataccga agggggcgta
     8341 atcgaactcc acgtccttct cggccggcgt gtgcggcgcg tgatctgttg cgatgcagtc
     8401 gatcgtacca tcgcgcaggc cttccttgat cgcctcgaca tcctcggcct cccgcaacgg
     8461 cggattcatc ttcgcgttgg tgttgaaggt cgtcacggcg tcatcggtca gggtgaaata
     8521 gtgcggagcg gtctcgcagg tcacgcggat gcctcgggcc ttggcccagc gcacgatctc
     8581 gacgctgccg gcgctgctga cgtggcagac atggactttc gcaccggtca tctcggccag
     8641 gcggaccagg cgggtcacgg cgatttcttc catggccttg ggcatgccgg ccaggcccag
     8701 cctggtggag ttgaggccgg cgttcatcgc gccgttctgg atcagcgagg tgtcctcgca
     8761 atggccgaga tacgtgaggc cgcacatgtc ggcgtattcc aggcagcgcc ggagcaccca
     8821 gctcgacgcg atatcgttgc cgtcatcggt gacggccacg gcgcccacgg ccttcagttc
     8881 ggccatttcg gtcatctcgg cgccggcccg gtccttggtg gcggcggccg cgggccagat
     8941 cttgatgcag gctgtctcgc gggcgcggcg gttgatgaat tccaccatcc cgccgttgtc
     9001 gatgggcggt tcggtattgg gcatggtcac cacggaggtg aacccgccgc gtgcggcggc
     9061 ctggctgccc gtagcgatgg tttctttcga ttcgaagccc ggctcgcgga aatgcacatg
     9121 gatgtccacc aggccggggc agacgatgca gtctgtagcg tcgaagacct cgctaccgct
     9181 cagatccgtg tcgatgcgcg caatgcgtcc gccgatgatc agcacatccc gcggcgccga
     9241 gaggttcgat gcggggtcaa tcacgtgtcc gttcttgatg acaatactca ttgccggcct
     9301 ccctatgcgg ccactcttgg tttgttgacc aggagatgca tgactgccat gcggaccgcc
     9361 accccgtttg tcacctgttc gagaattaca ttacgcggtc cgtcggcaac ctccgacgcc
     9421 agttcgatgt cgcggttgat cggcccggga tgcatgacca gggcgtctgg cttcgccgcc
     9481 ttgagcgatt cgttatcgat tcggaacagc cggatgtact cccggatact cggaaacagg
     9541 catttgtgct ggcgctccag ctggaggcgc agcagataga tgacgtctgc gccgtcgatg
     9601 gcctcacgaa gatccgtagt gacgtccaca cccagtttgg tgaaggcgtc cggaagaaga
     9661 gtgcgcggcc cgcagaggac gacgcttgca ccaagcttcg tcagacccca cacgttgctg
     9721 cgcgcgacgc ggctgtgggc gatatcgccg ataatggcga cccgcagccc atcgagaccg
     9781 gcttgagcgt cttcacgcgc gcggcggata tgctggcgga tggtgaaggc gtcgagcagg
     9841 gcctgggtag ggtgctcgtg tgcgccgtcg cccgcattga tgatgtggct gaggtgccgt
     9901 tcggccagga tgcgcgaggc gccggcggcg ctgtggcgaa tcacgacaat gtccgtatgc
     9961 atggcctcga tgttgtagag ggtgtcgtgg agcgtctctc cctttttggt gctcgaggcc
    10021 gaggcgttca tggtgagtgt gtcggcgctg aggcgtttgg cggcgagttc gaatgaggtg
    10081 cgcgtgcggg tgcttggctc gtaaaaccag ttcaccacga ggcggccctg gagcaccggc
    10141 accttcttga tgccgctttc ccgttccgac acggcgagaa actggggcgc cgtgtcgagg
    10201 atcatctcca gttcatcgcg cgacagctca tcgagaccga tcaggtcctt gcgggtccag
    10261 acgcgctgct cactctcgct cagcgtggcc atgtcactca ctctcctcgt cggctgcgct
    10321 gcgcgcccct ggctcgagaa ggaccgcgtc ctctccgtca atttcctgga tgcgcaccgc
    10381 cacatggtcg ttggcagccg tcgccagcgg ccatcccag
//
LOCUS       DAOPBQ010000054        10349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6262_length_10349_cov_4.390130, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000054 DAOPBQ010000000
VERSION     DAOPBQ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6262_length_10349_cov_4.390130"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..3409
                     /locus_tag="PKW60_03065"
     CDS             23..3409
                     /locus_tag="PKW60_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPJ98443.1"
                     /translation="MTVANDGKDLKIGNAVPFTYHRFSEGTAITAGASGEVTTNAFFF
                     NPNNRGGSGERELILSRTGSLITALGAVSLPSITAAELGQTDDLTSNVFVLVRSTKTP
                     RLFGTHEIEAGSPVANVWNFDIHLYEGVYPFSELGEVTFNTDPAQGEIRLATLTYPVD
                     DTTLTVGDLRDGTSTMSLDSTLSSLGFNAPPVYSYDPDSERYQSTVAASEFAAAGTDP
                     ASPVLQVTSRLAHLSAIALRTDVPAPSFTIQSIADSTTGLPRGPLQGGTTVIIRGQGL
                     GWPASVTFDGIPATNVTGVSDQKLTCTLPEGLVEEVPVDVAVTLVDGRSVTAAGAFTY
                     YLDIDFVITPNVGLPAGGNTVTFSGIGLLGVSQVLFGTQAATFTGGTDTRFTAVVPAA
                     SGPSVVSVTVTSTLGVRTINRAYTYIPQGGLRVTSIVVQGTDGPLPPGGGTLLINGQG
                     FSNVLRLRLIFSDGTEVTLVEGVDFFVDSDGQITLTLTPQRLAELANGAGLTNIPPGT
                     ATLLLDTAAGSAEIDDAFVVASDTTDVLVYEMTPDSAWVIGGFVAEIIGQNFGEPSVM
                     TAAFEYNTPSLFGKRVKSYDTVTVPLEILSGPEYPNNSTRLYVRIPALPGVNDAWPAV
                     IAMDRLVLTKGGVPVYGALPKDGAEKFGPIFTYYRWWEDDRGAVTTAFYYDNALGANA
                     REIMLNSGPLNAALTVPPLSSDFFNAKVTGNTDYVYVLARAGLDETVFGETDFTALPG
                     EGIDNAWVFDIHLYADAATTKALLPPARNLLYQEIAPQFVVTPDTDGLDVVPARLTIP
                     TNYTLLTAGDIRGGTIALYGQPSEVNDLVAPATVDATTALSDGVYQSTILESFAGLPE
                     FFPNAASAPDTQPVEKVRARVYEFSALTLRHGFDAAPDPILTSSARITGGRAEGPTSG
                     GTEVILYGTGLGWVETVEFGTGNNKAYSPATIVSGGDTNQFALRVISPAVRSAGRVNI
                     RITTAAGKQAVLQNGFTYTGRDSGIFALLLGLGMALTGMFAGGKGGDGGCFIATAAYG
                     TSMQGEIDVLRSVRDTYLLDNAVGTAFVDAYYRVSPFIAVAVAKSPVLAALVRVALTP
                     VIVVSKMLLALPHMSAGLGLLAMAGVMLRRLTRKAKTRKA"
     gene            4004..4273
                     /gene="fliQ"
                     /locus_tag="PKW60_03070"
     CDS             4004..4273
                     /gene="fliQ"
                     /locus_tag="PKW60_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HPJ98444.1"
                     /translation="MTVDTIVNLAREAMLVTLLTAAPMLLSGMLIGLLISVFQSVTQI
                     QEITLTFVPKIVVVMVVFVVFLPWMVGVVMSFVQPLLSHFNVLVL"
     gene            4277..5047
                     /gene="fliR"
                     /locus_tag="PKW60_03075"
     CDS             4277..5047
                     /gene="fliR"
                     /locus_tag="PKW60_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HPJ98445.1"
                     /translation="MLFEAEVFKVFVLVFIRISGLLVSAPVLGSANFPARAKIGLAVL
                     LAALLTPTIGRLQEPLPGDYLVLGVMAGAELGIGLLMGFVMTLVFAAIQVAGEVMDML
                     SGFSLMNVFNPALETQVPIFGFFLYIVAALYLLTVDGHHLMIRGLVRTFSTMPLGQVA
                     LRPEILGQAARWGSLMFVDGLLIAAPVAGALLLAYVTMGLMGRVVPQIHLFAVGFPFT
                     IALALLMTALFIGVYADILSGMFHRMFQGVSSLTQGMT"
     gene            5055..6179
                     /gene="flhB"
                     /locus_tag="PKW60_03080"
     CDS             5055..6179
                     /gene="flhB"
                     /locus_tag="PKW60_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HPJ98446.1"
                     /translation="MPEQTGGEKVLPASPRKITKAREEGRVARSQDLSSAWSLCIALL
                     TLYLLGSSAAAGMVSISRHYFQEASLLADELANPQWVALGALARMARILLPFLLVMLA
                     GGLAMNLAQVGFLFAPAALTPRFERLNPFSGFQKFLNLRSFIELVKSVVKLTAIGAIV
                     YVTFRSRWQELLAWPVLTPAGLAGAMASLVGLVWLRVVLAMLVLGILDYGFQRWQYLQ
                     ELRMTVQEAREEMKEIEGDPRIRQRIRQIQRQMAMQRMMRAVPEADVIVTNPTHVAVA
                     LRYNAQEMEVPVVTAKGARLLAERIRALAEEHEVPIVQKPDLARMLYRTLEVGQPVPE
                     NLFVAVAEILAFVFEIDRRAGKLGERSGMLHGLRQRRAAG"
     gene            6201..8282
                     /gene="flhA"
                     /locus_tag="PKW60_03085"
     CDS             6201..8282
                     /gene="flhA"
                     /locus_tag="PKW60_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HPJ98447.1"
                     /translation="MAVVPQEMDQGRWSFARNQDIVLAVAVMGVLAVLVIPIPTALLD
                     ILLAINISLSVVVLLTAIYLQHPVDFAVFPSLLLMLTLFRLSLNVASTRLILSQANAG
                     SIINAFGSFVTSGSYIVGIIIFVILVVIQLVVITRGATRISEVAARFTLDAMPGKQMG
                     VDADLNAGLITEDQARSRRRRIEQEADFYGAMDGATKFVRGDAIAGVIITLVNIIGGL
                     AIGVLVHKYELGAAAQVYTQLTVGDGLVSQIPALIVSMAAGLIVTRTASQENLGVDMG
                     RQLGAYPRALGIAAGLLVLFGIVPGMPAIPFFVVGIILAITGYQTHAAIARRAVLETA
                     REAAVAETAATEGPERTEYLLPVDPLKIELGYGLIALADPKQGGDLLNRIQIIRQQMA
                     TRMGFIVPVVRIVDNMRLRPNEYRVKLRESEIARFELMPGHYLAMNPGIVEEEIAGIP
                     TTEPAFGLQALWVSAENRDRAERMGYTMVEPSAVLATHLTELITTHAPELLTRQDVQN
                     LVNVVRESARAVVEELTPNVLSLGEIQKVLQNLLRERVSIRNLETILEVLADFAPRTH
                     DAEVLTEYARQALARQICADYVDERNALHLATLAPDLEREILDAVQQSETGEYIPLSP
                     ARADAIARKTVDAVQPLVMSGQDPIVLTSAPVRRYFRRIVERFLPRIVVLSYNEIDPA
                     VRLESEGQVNG"
     gene            8275..9744
                     /gene="flhF"
                     /locus_tag="PKW60_03090"
     CDS             8275..9744
                     /gene="flhF"
                     /locus_tag="PKW60_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HPJ98448.1"
                     /translation="MDRETLGFRKFRAPTFDEALRQMRAVLGESAVVLRTTQVKEGGL
                     FGLFGEPLVEITAAGAAPPAPDPGARTNLESRPPSAVERRYKAHSAVGSDQRVRDTVE
                     FCRELVTRAQSRLETRRNADNARAASAPGAPGAPSGSQTAARAVSVPPRRHGSDGKGP
                     YSRPRKTAYPSDGNAAIAAAPFTPLHGRTQAVGPDPSRDELRELLRVLEGNASSAGLP
                     TELAPHYRRLVEAGVSSSLAASLLGAAVRGCNVGVLSDERVLRERLRLELRRRVSVKR
                     GICLQADRCTVVALVGPTGVGKTTNLAKLAAQFAVRDRRQVALVTADTYRVAAPDQLR
                     VYADIIGLRMVVVNEPREMRQALKELQNCDLVLIDSAGSSQFNGEQLAELQRMMAAAE
                     PSEVLLAIAANTPLEDLRSICENFGKVNPTALMVTKIDEARRFGTMCSIALETGLPLS
                     YFSVGQNVPDDILLAEPAMVANLVLEGRDGSGRSSRAAS"
     gene            9716..>10349
                     /locus_tag="PKW60_03095"
     CDS             9716..>10349
                     /locus_tag="PKW60_03095"
                     /inference="COORDINATES: protein motif:HMM:NF020704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPJ98449.1"
                     /translation="MADQAEQLREYVRGNRRQARVLAVTSGKGGVGKTGTSVNLSIAL
                     AMRGKEVVVLDADLGLANVEVLLGLNSLHNLQHVINGERRMPEILAEGPGGIKVVPGS
                     SGLAKLADLGPHARQRVIDGMQELQSCADFIMIDTMAGISQNVAAFAAAADEILLVVT
                     PEPSSIVDAYAMLKTIRMMRHDAVFKLVVNMVASEQQARAVAGKLMTVSQQ"
BASE COUNT         1854 a         3048 c         3468 g         1979 t
ORIGIN      
        1 accgttctcg gagactgttc aggtgaccgt ggcaaatgac ggcaaagacc ttaagattgg
       61 aaacgcggtg ccgttcacct atcaccggtt cagcgagggc acggccatta cagccggcgc
      121 cagcggcgag gtgacaacca acgcgttctt cttcaacccg aacaaccgag gcggctcggg
      181 cgagcgggag cttatcctgt ctcgaaccgg ctcactaatc accgccctgg gtgcggtgtc
      241 gctgccgtca attaccgcgg cggagctggg ccagacggat gacctcacga gcaacgtgtt
      301 cgtgctggtg cgctcgacga agacgccgag gctcttcggc acgcacgaga tcgaagccgg
      361 ttcgccggtg gcgaatgtct ggaatttcga catccatctg tatgaaggcg tctacccgtt
      421 ctccgaactg ggcgaagtca ccttcaacac cgatcccgcg cagggcgaaa tcaggctggc
      481 cacgctgacc taccccgtcg acgataccac gctgacggtg ggcgatctgc gcgacggcac
      541 ctcgacgatg tcgctggaca gcacgctgag ctctcttgga ttcaacgcgc cgccggtcta
      601 cagctacgac cccgacagcg agcgctatca gtcgaccgtg gcggcatctg aatttgcggc
      661 ggcaggcaca gatccggcca gcccggtgct tcaggtcacc tcgcgtttgg cgcatctcag
      721 cgcaattgcg ctgcggaccg acgtgccggc gccgagcttc acgatccagt cgatcgcaga
      781 cagcacgacc ggattgccga gggggccgct gcaaggcgga acgacggtta tcatccgcgg
      841 ccagggcctg ggctggccgg ccagcgtcac gtttgacggc atcccggcga cgaacgtcac
      901 cggcgtgtcg gaccagaaac tgacctgcac cctgccggag ggcctcgtgg aagaggttcc
      961 ggtggatgtg gcggtcacgc tggttgacgg gcgcagtgtg acggcggcag gcgccttcac
     1021 gtactacctg gatatcgact tcgtgatcac gccgaacgtg ggcctgcccg cgggcggcaa
     1081 tacggtcaca ttcagcggca tcgggctgct gggcgtgtcg caggtgctct tcggcaccca
     1141 ggcggcgacg ttcaccgggg gaacggacac ccggttcacg gcagtggtcc ccgcggcctc
     1201 gggcccgagc gtggtttccg tgacggtgac ctccactctg ggcgtccgca cgattaatcg
     1261 cgcgtacacc tacattcctc aaggcggatt gagggtcacc agcatcgtgg tgcaggggac
     1321 cgatggcccg ctgcctccgg gtggcggtac gctgctcatc aacggccagg gcttcagcaa
     1381 tgtgctgcgc ctgaggttga tcttcagcga cgggaccgaa gtcacgctgg tggaaggcgt
     1441 cgacttcttc gtggacagcg atggccagat tacgctgacc ctgacacctc agcgcctggc
     1501 tgaactggcc aacggagccg gcctgaccaa cattccgccg ggtacggcaa cgctgctgct
     1561 cgacacggcc gccggttcgg cggagatcga tgacgccttt gtcgtcgcga gcgacaccac
     1621 cgacgtgctg gtctacgaaa tgacaccgga cagtgcctgg gtgatcggcg ggttcgtggc
     1681 cgagatcatc gggcagaatt tcggggaacc gtcggtgatg acggcggcct tcgaatacaa
     1741 cacgccgtct ttgtttggca agagagtcaa gtcgtacgac acggtaacgg tgccgctgga
     1801 aattctgagc ggccccgagt atccgaacaa ctcgacgaga ctgtacgttc ggattcccgc
     1861 gctgccgggc gtgaacgacg cctggccggc ggtgatcgcc atggatcggc tggtgctgac
     1921 gaagggcggc gtgccggtct acggcgcact gcccaaggat ggcgccgaga aatttggccc
     1981 gatcttcacg tattaccgct ggtgggaaga cgatcggggt gcggttacga cggcgttcta
     2041 ctacgacaac gcgctgggcg cgaacgcacg cgagatcatg ttgaacagcg gcccgctgaa
     2101 tgcggcgctg actgtgcccc cgctgagcag cgatttcttc aacgcgaagg tgacgggcaa
     2161 cacagactac gtgtatgtgc tggcgcgtgc gggtctggat gagacggtgt tcggcgagac
     2221 cgatttcacg gccctgccgg gcgagggcat cgacaatgcc tgggtcttcg acatccatct
     2281 gtatgcagat gccgcgacca ccaaagcgct gttgccgccc gcccgcaacc tcctgtacca
     2341 ggagatcgcg ccgcaattcg tcgtgacgcc tgacaccgac ggcctggacg ttgtgccggc
     2401 acgcctgacg atcccgacca attacacctt gctgaccgcc ggcgatatcc gcggcggaac
     2461 cattgccctg tacggacagc cgagcgaggt caacgacctg gtcgcaccgg cgactgtgga
     2521 cgcaacgacg gccctctccg acggcgtgta ccagtcgacc attctggaga gcttcgccgg
     2581 cttgccggag ttcttcccga atgcggcttc tgcgccggat actcagccag tagaaaaggt
     2641 gcgtgcgcgt gtctatgaat tcagcgcact cacactgcgg cacggattcg acgcggcgcc
     2701 cgatcccatc ttgacctcga gtgccaggat aaccggcggc cgcgcggaag gcccgaccag
     2761 cggcggtacc gaggtgatcc tgtacggcac cggcctgggc tgggtcgaaa cggtcgagtt
     2821 cggcacgggc aacaacaaag cctacagccc ggcgacgatc gtcagcggtg gcgacaccaa
     2881 ccagttcgcg ctgcgggtga tctcgcccgc agtaaggtcg gccggccggg tcaatatccg
     2941 catcacgacg gccgcgggca aacaggccgt gctgcaaaac ggcttcacct acaccggccg
     3001 cgacagtggc attttcgccc tgctgctggg cctcggcatg gctctgaccg gcatgttcgc
     3061 cggcggtaaa ggtggcgatg gcggctgctt cattgcgacc gccgcgtatg gcacctcgat
     3121 gcagggcgag atcgacgtcc tgcgcagcgt acgcgacacc tatctgctcg ataacgccgt
     3181 aggcacggcg ttcgtcgacg cctattaccg ggtgagcccg ttcattgccg tggccgtcgc
     3241 gaagagcccg gttctggcgg ccctggtccg ggtagcactg acaccggtca ttgtggtgag
     3301 caagatgctc ctggcactgc ctcacatgtc ggcaggcctc ggattgcttg ccatggccgg
     3361 cgtgatgttg cgcaggctta cacgtaaggc gaagacgcgt aaagcctgag gctggtacgt
     3421 gacataagcc ccgacactcc ggcgggtttc gaaagaaccc gccggagtct tttttgtgca
     3481 ccctgcagaa cgcactctct tggaggtgaa agtcctctac aggcccggca ccgggaacct
     3541 atccggatgg tacagcatgg gaataacaat tgtggcgact agagggcttc atatggacga
     3601 tacgagccac ttggagggat tttcgacgca tgtgcgggga aaccgcctgg gaacgccgac
     3661 gtccccgccg gcagcttggg aacgccgacg tccccgccgg cagcttggga acgccgacgt
     3721 cctcgtcggc aactcatttt gtcggcgcgt gtgcggggaa accgtcgttt ccactgaata
     3781 ccgttgtttt cctgaataat cgcccggtaa accaccctct acagggcgaa aaccctgtag
     3841 tctgaccggg ggcctctttc gcctaaatca ttgtgaaata acgcaataaa ttgagttcag
     3901 ttcgttgaca accgctatat gttgtgttat tttgtcaatg taccgaaatg taagcgctct
     3961 gcggccacac atggtgctgc attgactgtg cgaggggacg gaagtgacgg tagacacgat
     4021 cgtcaatctg gcgcgagaag ccatgctggt gacgctgctg accgcggcgc cgatgttgct
     4081 gtccggcatg ctgatcgggc tgctgatcag cgtgtttcag tcggtcacgc agattcaaga
     4141 gatcactttg acgtttgtgc cgaaaatcgt ggtggtgatg gtggtgtttg tcgtgttttt
     4201 gccgtggatg gtcggcgttg tgatgagttt tgtgcagcct ctgctcagcc acttcaacgt
     4261 gctggtactg taacccatgc ttttcgaagc ggaagtcttc aaagtattcg tgctcgtgtt
     4321 tatacggatc agcgggctgc tggtgagcgc gccggtgctg ggctcggcca attttcccgc
     4381 gcgcgcgaag attgggctgg cggtgttgct cgccgcgctg ttgacgccca ctattgggcg
     4441 ccttcaggag cctttgccgg gcgattacct cgtgctgggc gtgatggccg gcgcggagct
     4501 tggtatcggg ctgctgatgg gatttgtgat gactctggtg tttgcggcga ttcaggtcgc
     4561 gggcgaggtc atggatatgc tgagcggttt ctcgctcatg aacgtgttta accccgccct
     4621 ggaaactcaa gtgcctatat tcggcttctt cctgtacatt gtcgcggccc tgtacctgct
     4681 caccgtagac gggcaccacc tgatgattcg gggactggtt cgcacgttct cgacgatgcc
     4741 gctgggccag gtggcgctgc ggccggagat tctggggcag gctgcgcgct ggggcagcct
     4801 gatgtttgtc gacgggctgc tgatcgccgc gcccgtggcc ggcgctctgc tccttgcgta
     4861 tgtgacgatg gggctgatgg gccgcgtcgt gccgcagatt cacctctttg cggtgggatt
     4921 cccgtttacg atcgccttgg cgctcttgat gacggctctg ttcatcgggg tctacgcgga
     4981 catcctgagc gggatgttcc accggatgtt tcagggcgtc tcctctttga cacagggaat
     5041 gacgtagacc acccatgccc gaacagaccg gaggcgaaaa agtacttcca gcttccccgc
     5101 gaaagatcac gaaggcgcgg gaggaaggcc gggttgcgcg cagtcaggat ttgagcagcg
     5161 cgtggtcgct gtgcatcgcg ctgctgaccc tctatttgct gggttcgagc gcggcggccg
     5221 ggatggtgtc gatctcgcgg cattatttcc aggaggcgtc gctgctcgcg gacgaactcg
     5281 cgaatcctca gtgggtggcg ttgggcgcgt tggcgcggat ggctcggatc ctgctcccgt
     5341 ttctgctggt gatgctggcg ggcggcctgg ccatgaacct ggcgcaggtc gggtttttat
     5401 ttgcaccggc agcgcttacg ccccgctttg aacggctcaa tccgttttca gggtttcaga
     5461 agtttctgaa tctgcgatcg tttatcgagc tcgtgaaatc ggtggtcaag ctgactgcaa
     5521 ttggcgccat tgtctacgtt acgttccgca gccgctggca ggagcttctc gcctggccgg
     5581 tattgacgcc cgcgggactg gccggcgcga tggcttcgct ggtgggcctg gtgtggttgc
     5641 gggttgtgct cgcgatgctg gtgctgggca ttctggacta cggatttcag cgctggcagt
     5701 acctgcagga actccggatg accgtgcagg aggcgcggga agaaatgaag gagatcgaag
     5761 gcgacccgcg aatccggcag cggatccgcc agatccagcg ccagatggcg atgcaacgca
     5821 tgatgcgtgc cgtgcccgag gccgacgtga tcgtgaccaa cccgacgcat gtggccgtgg
     5881 cgctgcggta caacgcgcag gaaatggaag tgcctgtggt gacagcaaaa ggagcccggc
     5941 ttctcgcgga acgcatccgt gcgctggcgg aggaacacga ggtccccatc gtgcaaaaac
     6001 cggatttggc ccggatgctc taccgcacgc tcgaggtggg ccagccggtg ccggaaaacc
     6061 tgttcgtcgc cgtggcggaa atcctcgcct ttgtgtttga gattgaccgc cgcgccggaa
     6121 aactcggaga gcggtcgggc atgttgcacg ggctccggca acggcgagcc gcgggctaat
     6181 agccgcaacg ggagcggtac atggctgttg taccacaaga gatggatcag ggccggtggt
     6241 cgttcgcccg caaccaggac attgtgctgg cggttgcggt gatgggcgtc ctggcggtgc
     6301 tggtgatccc tatcccgacg gcgctgctgg acatcttgct cgccatcaat atctcgctgt
     6361 ccgtggttgt gttgttgacg gcgatctatc tgcagcatcc ggttgatttt gctgtatttc
     6421 cgtcgttgct gctgatgctg acgctgttcc gcctgagcct caacgtggcc tccacgcgcc
     6481 tgattctcag ccaggccaat gccgggtcga ttatcaatgc attcggaagc tttgtgacca
     6541 gcggcagcta tatcgtcggc atcatcatct tcgtcatcct ggtggtgatc cagctggtgg
     6601 tcatcacacg cggcgccacg cgcatctccg aagtcgccgc acgcttcacg ctggacgcca
     6661 tgccgggcaa acagatgggc gtggacgcgg atctcaacgc aggattgatc accgaggatc
     6721 aagctcggtc gcgccggcgc cggatcgagc aggaagccga cttctatggc gcgatggacg
     6781 gcgcgaccaa gttcgtgcgg ggcgacgcca tcgccggggt gatcatcacg ctggtcaaca
     6841 tcattggggg cctggccatc ggggtcctgg tccacaaata cgaattgggc gccgcggctc
     6901 aggtctatac ccagctcacc gtgggcgacg gcctggtttc gcagatcccc gcgctgatcg
     6961 tttcgatggc ggcgggcttg atcgtaacgc gcacggcatc ccaggagaac ctgggcgtgg
     7021 acatggggag gcagctgggg gcctatccgc gtgcgctggg gatcgccgcc gggttgcttg
     7081 tattgtttgg catcgtgccg ggcatgccgg ccatcccgtt ttttgtggtg ggcattattc
     7141 tcgccatcac gggctatcag acgcacgcag ccatcgcacg gcgagctgtc ctggaaacag
     7201 cgcgcgaggc ggccgtggcg gaaaccgcgg ccacggaagg accggaacgc accgaatacc
     7261 tcttgcccgt ggacccgctc aaaattgagc tgggctacgg cctgatcgcc ctggccgacc
     7321 cgaaacaggg cggcgacctg ctgaaccgta ttcaaatcat ccggcagcaa atggcgacgc
     7381 ggatggggtt catcgtgccg gtggtccgta tcgtggataa tatgcggttg cggcccaacg
     7441 aataccgggt caagcttcgc gaatcggaga tcgcgcggtt tgaactgatg cccggacatt
     7501 acctggccat gaatcccggc atcgtggagg aagagatcgc tggcattccg accacggaac
     7561 ccgccttcgg cctgcaggcg ctgtgggtca gcgccgaaaa ccgtgaccgc gcagaacgga
     7621 tgggatacac catggtcgag ccgtcggcgg tcctggccac gcacctcacg gaactgatca
     7681 cgacccatgc gcccgaactc ctgacccggc aggacgtgca gaatctggtc aatgtagtgc
     7741 gggagtcggc gcgggccgtg gtggaagaac tgacaccgaa tgtgctgtcg ctgggagaga
     7801 ttcaaaaagt gttgcagaac ctgctgcgtg agcgggtgtc gatccggaac ctcgagacca
     7861 tccttgaagt gcttgcggat ttcgcgccgc gcacgcacga cgcggaagtt ttgaccgaat
     7921 atgcgcggca ggcgctggca cggcagattt gcgcggatta tgtggatgaa cggaatgcgc
     7981 tgcatctggc gacgcttgcg ccggacctgg aacgggagat cctggatgcg gtgcagcaat
     8041 cggaaacggg ggaatacatc ccgctgtcgc ccgcccgggc agacgcgatc gctcgaaaga
     8101 cggtggatgc cgtgcagcct ctggtgatga gcgggcagga tcccattgtg ctgacttcgg
     8161 cgccggtgcg gcggtatttc cggcgcattg tggagcgctt cctgccgcgt atcgtggttt
     8221 tgtcgtataa cgaaatcgac ccggctgtgc gactggaaag cgaagggcaa gtgaatggat
     8281 agggaaacgc tgggattccg caaattccgg gcgccgacgt ttgatgaggc gctgcgccag
     8341 atgcgcgccg tgctgggaga gagtgccgtg gtcctccgca cgacgcaggt caaagaaggc
     8401 gggctgttcg gcctcttcgg cgagccgctg gtggaaatta ccgccgccgg cgctgctccg
     8461 cccgcgccgg acccgggcgc gcgcaccaat ctcgaatcgc gaccgcccag cgccgtggag
     8521 cggcgctaca aagcccattc ggcggtgggc tctgatcagc gggtccgtga caccgtggag
     8581 ttctgccgtg aactggtgac tcgggcacag tcgcggctgg agacgcgccg caatgcggac
     8641 aacgcccggg cggcttccgc tccgggcgcg ccgggggcgc cgtcgggttc acaaacagcg
     8701 gcccgggctg tgagtgtgcc gccgaggcgg catgggagcg acggaaaagg cccgtattca
     8761 cgaccacgga aaacagcgta tccctcggat ggcaatgcgg cgatcgcagc ggcgccgttc
     8821 actccactgc acggccggac gcaggccgtc gggcccgatc cgtcgcgcga cgaactccgg
     8881 gagctcctcc gggtgctcga gggcaatgcg tcgagcgcgg gtctcccgac cgagctggcc
     8941 cctcactacc gccgtttggt cgaggcgggc gtctcctcgt cgctggcggc ctcgctcctg
     9001 ggagccgcgg tgcggggctg caatgtgggc gtcttgagcg acgagcgggt ccttcgcgag
     9061 cggctgcggc tcgagcttcg ccggcgggtg agtgtaaaac gggggatctg cctccaggcg
     9121 gaccggtgca cggtcgtggc gctggtgggg ccgacaggcg tgggcaagac cacgaacctg
     9181 gcgaaactgg ccgcccagtt tgcggtccgg gatcggcggc aggtcgccct ggtgaccgcg
     9241 gacacctacc gggtcgcggc gccggatcaa ttgcgcgtct acgcggacat catcggcctg
     9301 aggatggtcg tggtgaatga gccgagagaa atgcgccagg cgctgaaaga gctgcagaac
     9361 tgcgacctgg tgctgatcga ttcggcaggc agcagccagt tcaatggcga gcagctggcg
     9421 gagctgcagc gcatgatggc ggctgccgaa ccgtcggagg tcctgctggc aattgcagcc
     9481 aacacgccgc tggaggacct gcggagcatc tgcgaaaact tcggcaaggt gaatccgacc
     9541 gcgctgatgg tgaccaagat cgatgaggcc cggcgcttcg ggacgatgtg ctcgatagca
     9601 ctcgaaaccg ggctgccgtt aagttatttc agtgtgggac agaatgttcc ggatgatatc
     9661 ctgttggcgg aacccgcgat ggtggcgaac ctcgtactgg aaggcaggga cggaagtggc
     9721 agatcaagca gagcagcttc gtgaatatgt tcgcggcaac aggcggcagg cccgcgtcct
     9781 ggcggtcacg agcggcaaag gcggcgtagg aaagacaggg acgagcgtca acctgtccat
     9841 tgcgctcgcg atgcgcggaa aagaggtcgt ggtgctcgat gccgatttgg gcctggcgaa
     9901 cgtcgaggtt ctcctgggcc tgaattcgct tcacaatcta cagcacgtga tcaacggcga
     9961 acgccggatg ccggagattc tggccgaggg cccgggaggc atcaaagtgg tgccggggag
    10021 ctcgggtctc gcaaaactgg ccgatctggg accccatgcg cgccagcggg tgattgacgg
    10081 catgcaggaa ctccagagct gcgccgattt catcatgatc gacacgatgg ccgggatcag
    10141 ccagaatgtc gcggcatttg ccgccgcggc ggacgaaatc ctgctcgtgg tcacgccaga
    10201 accctcgtct atcgtggacg cctatgccat gctgaagacg attcggatga tgcgccacga
    10261 cgcggtgttc aaactggtgg tcaatatggt cgcgagtgag cagcaggccc gtgcggtcgc
    10321 cggaaaactc atgaccgttt ctcagcagt
//
LOCUS       DAOPBQ010000055        10275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6320_length_10275_cov_3.292955, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000055 DAOPBQ010000000
VERSION     DAOPBQ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10275
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6320_length_10275_cov_3.292955"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2737)
                     /locus_tag="PKW60_03100"
     CDS             complement(<1..2737)
                     /locus_tag="PKW60_03100"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPJ98450.1"
                     /translation="MKCLQTIVTVLTLVAAACAAGASDMVMWAPARDQYPTQDPGGTS
                     ILPNGRTITPDGAMFRVRPHPYGLTMSKDGRWVVAVSSDGPQLSILDMKDPSAPEIFF
                     IPNDQNEGEGVLDAAFMGLAIAPDNKTLYVAGGKDWSVTAFDLETREHLFRIDCAHDA
                     DGQNFRHGYAGDLRLSHDGKTLYVCDQSNFRMLILDTAQRAVVRSIPVGRYPFGIALS
                     PKGDHAYVANVGMYEYSYFRTEDGEYAKLPFPPFGVPSKEAEEGVTIDGVVVSGLGDP
                     NDIAGMSVWKIRISRSGKEKVVKKTKTGHLVGELLEDFPAVGGSSPNSIAATRSHVYV
                     SNGSNDSISVIHARTGEREKDIDLILHPLAAKLRGMIPFGVALSPDHSMLYVAEAGVN
                     AIGVIRLSDHKVLGHIPSAWFPSKLAVSPDGKRLYVACAKGVGSGPNAGPNHKEGDPT
                     GIGALMRGYIHIINLPQSQDELDALTQRVIENNVVIKPVEALARPKNHPVPPVPGAWQ
                     SPVKHVIYVTKENRTYDEIFGAMPGGRGDAELCRLGRPIDVTNKDGTRTVKDVVLMPN
                     HIALAKRFAMNDNFYCDSDHSVDGHRWLVGTYPNEFMEARIGESAEGNGPGNFLFIGS
                     SGAIYPEDYNEAGSIWEHFDRNGLQFFNFGLGFEFKPQEETQEDRYTGIRLNTNYPMP
                     QPLFENTSRIYATYNTNIPDQFRMDMFEQEYREKWLSGAEPFPDVITIMLPNDHGAGE
                     RPNDGYPFWGSYMSDNDLALGRLVELISHSPWWKETVIFVTEDDAQGYRDTVDAHRSI
                     CMVISPYAKQGHVSHIHSSFGSILKTMFLIHGVPPLNQYDAFSCDLAEMFDSEPVNAA
                     PYKALPVNSEIFDPQKAFDPFDREFNWQALNEFVPLDHQEFLDSDTYGHSGDTGHG"
     gene            3248..5875
                     /locus_tag="PKW60_03105"
     CDS             3248..5875
                     /locus_tag="PKW60_03105"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPJ98451.1"
                     /translation="MRTRTLIVCVAVVLVGCATHELPGVQRALDAGPDAFLDQAVVGP
                     QGDGTFVVPTTQLLNPAGDTVEFPGRPLGIAVHPDGRLLAVKNRTDVVFFDAVDRRVV
                     QTLPLPKGGSSFCGIAWSADGNRVWTTAAQAAIHCAERGGGGAFEWVKTINLPGPDGK
                     GASAPGGFALDERGGLIYVALSRNNSVGVVDISSEAVLTEIPVGMAPYDVLLHGGKAY
                     VTNWGGRRPTPDDITGPSSGSQVVTTPAGIASTGTVSVLDLEARRTSQEIEVDLHPCG
                     MALSPDGATLYVANANSDTISVIDTADHVVKGRWIARPSLELPFGSAPNAIALQDNGE
                     SLFVALGGNNCIAVMSARDGRVTGLIPTGWYPGAVAFAQDGALLCVANTKGAGSRYLE
                     ENTTRPESERYPGVRGRSSHDHLGSVTIVRTPNESTLDAYTHQAAVAMRLPHMLRDAG
                     AAEHPKRVVAIPERPGDVSPIKHVLYIIKENRTYDQVFGDMPQGNGDPTFCHFGREVT
                     PNHHALAEEFVLLDNFYCNGVLSADGHQWTDEGYVTDYLEKAFGGWSRSYPYDGGDAL
                     AYASTGFIWDHVLAKGLTFRNYGEMVKPRITPNDATWADIYQDYLNGTATVKIEAYTE
                     VRGLEGYLCPTYIGFPGKVQDVYRVREFLKEFRACEEKGEWHNFVIMLLPNDHTVGTR
                     EGFPTPNAAVADNDLALGQLVEAVSHSQFWPETAIFVVEDDPQAGLDHVDGHRTVALC
                     ISPYTRRGEVVSTHYNQSSMLRTMELIMGLPPMSQFDMAANPMYACFTNTPDLTPYTC
                     RPNQIPLDEMNPKLASLRGKQLYYAQKSMEMPLDDIDQADEDLFNRILWHSVKGYDVP
                     YPKLARRASSEAVVWGLKD"
     gene            5925..6821
                     /locus_tag="PKW60_03110"
     CDS             5925..6821
                     /locus_tag="PKW60_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HPJ98452.1"
                     /translation="MHKKTGFTLIELLVVIAIIGILAAILLPALSRAREAARRAACAN
                     NLKQMGLALYMYADEHRGRFPHRQVWTVTGGLSREMMFNGPAMIPEYISDVNVVWCPS
                     WNKPPDIVLRYDGGKGNGDGIIQPEEVGQEPYHYTGWLIMDDVNILGPLVGVIGNGIN
                     GRHDENEYVVTPWGELAQANVDTGGAASDSDFTVSATHAGTQVGGGSTYYRLRQGIER
                     FMVTDINNPASSAAACSIVPLMWDHVSTNPKDFSHIPGGGNVLYLDGHVEFLRYPSDR
                     FPFTPDSARTLGKYGKIFDGID"
     gene            7316..>10275
                     /locus_tag="PKW60_03115"
     CDS             7316..>10275
                     /locus_tag="PKW60_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPJ98453.1"
                     /translation="MAGLKTSETQWARPWLIWTVVLFVLAGAVLEFASLYADANQVPP
                     RNLGNFLSLVSAVFLASAGVVLVRHLKYERASRTIMEVAAGLFIYSKVIAWAGRLPTL
                     STVPLFGIHGPGYEVLESNTHTIALLLVLIGFIRSMWVANKTRHDLEVQNQALLHEVE
                     QRTSVERELRASEIRYRDLVAEIPDVVWRLDTTGVFTFASPAVEMVFGMRPDAVVGKH
                     LADVLPPALAERALASLTKKIQTEPGEALVPFQFHQSLPDGRVRFFEAMSAPVYSLEG
                     QIEEIQGILRDITAHIEANEALAASKERYRAFVENSPMVIVRTDRNLKCIFANRASLD
                     IAGLTRDEVLGKGQEAIQDVMHPDDHALIVTHCHEVLATEERRATEVRFRDPSGQWRW
                     FAHLVYPWYEPNGQLGGIEASARDVTEQKRDQEALRQSEETARALLNATSDLVGLLDE
                     DGRLLACNEHFAASVGIPASELVGANYRELVQGDTGRKRWENAVAVMRTGRQILFSDD
                     YNGRHFEHCFYPVRDEQGKCTRLAFYSRDVTTFLRKEEERIRLAAAIEQAPDLVIITD
                     ADGFVQYVNPGFERNTGYAREDVAGKTTRFLRSSEHDDAFYEAIQGTISRGVTWSGRM
                     VCRRKDESLFESDATVSPVQNAAGVISNYIWLMHDVSTEVSLERQLRQAQKMEAVGTL
                     AGGIAHDFNNILSLILGYSELLLDLLADNEPALLNVRQIAKAGNRGAELVRQILTFSR
                     QAERRCEPCRLGPILKESLRFLAASLPAAVEIQQRIGEETGSVMADPIQIYQTIMNLC
                     TNAYQAMGNGGGVLELTLDEVKIDASFHADAGSPEPGKYVRLTVSDTGTGIEPGDIGR
                     IFEPFFSTKKPSQGTGLGLSTVHGIVTGYGGAVTVRSVVGEGTVFEVYLPQMDAEAPV
                     ERVSEEYPGAGTERILFVDDDPDLSQMAGMMLQQLGYRVETFTNSLAAFEAFRARPVK
                     PFANLTGAYIVAPFG"
BASE COUNT         2067 a         2809 c         3251 g         2148 t
ORIGIN      
        1 ggccgtgtcc ggtatcgcct gaatggccgt atgtgtcgct atccagaaac tcctggtgat
       61 ccagggggac gaattcattg agcgcctgcc agttaaattc cctgtcgaag gggtcaaacg
      121 ccttctgggg gtcgaaaatt tccgagttga cgggcagggc tttgtacggg gcggcgttca
      181 cgggttccga gtcgaacatt tcggccaggt cgcatgaaaa ggcgtcatac tgattcaacg
      241 gcggcactcc gtggatcaga aacatcgtct tgaggatgct gccgaagctc gaatgaatgt
      301 gtgagacgtg cccctgcttg gcgtaggggc tgatcaccat gcagatggac cggtgggcgt
      361 ccacggtatc acggtatccc tgcgcgtcgt cctcggtgac gaagatgacg gtttccttcc
      421 accaggggct gtggctgatg agctcaacca gccgcccgag cgcgaggtca ttgtcgctca
      481 tgtacgatcc ccagaagggg taaccgtcgt tgggccgttc gcccgcgccg tggtcgttgg
      541 gcagcattat cgtgatcaca tcggggaagg gttcagcgcc ggacagccat ttttcacgat
      601 attcctgctc gaacatgtcc atccggaact gatcggggat atttgtgttg tacgtcgcat
      661 agatgcgcga ggtgttttca aacaggggtt ggggcatggg gtaattggta ttgagtcgaa
      721 tgccggtata ccggtcttcc tgtgtttctt cctggggctt gaattcgaag cccagcccga
      781 aattgaagaa ctgcaggccg ttgcggtcga agtgttccca gatcgagccg gcctcgttgt
      841 aatcttccgg ataaattgcg cccgacgatc cgataaacag gaagttgccc ggcccgttgc
      901 cctcggcgga ttcgccgata cgtgcctcca tgaattcatt cgggtaggtg ccgaccagcc
      961 agcgatgtcc gtcaacgctg tgatcggaat cgcaatagaa gttgtcattc atggcaaagc
     1021 gctttgccaa ggcgatgtgg ttgggcatca gcacgacgtc tttgaccgtt cgggtgccgt
     1081 ccttgttggt gacatcaatg gggcggccga ggcggcagag ttccgcgtcg cctcttccgc
     1141 cgggcatcgc cccgaagatc tcgtcgtagg tgcggttttc tttcgtgacg tagatcacgt
     1201 gtttgacagg gctttgccat gcgccgggca caggggggac aggatggttt ttggggcggg
     1261 ccagggcttc gaccggtttg atcacgacgt tgttctcgat gacccgttgg gtgagggcgt
     1321 ccagttcgtc ctgcgattgc ggcaagttga tgatgtggat gtagccgcgc atcagcgcgc
     1381 cgatgccggt ggggtcgcct tctttgtgat tggggccggc attgggaccg gaacccacgc
     1441 ctttcgcgca ggcgacgtaa aggcgtttgc cgtcgggaga cactgccagc ttactgggga
     1501 accaggcgga ggggatgtga cccaggactt tgtggtcact caggcggatt acgccaatgg
     1561 cgttgactcc cgcctccgcg acgtacagca tggaatgatc cggcgaaaga gcgacgccga
     1621 agggaatcat cccgcgcaac tttgccgcca gtggatgcag aatgagatcg atgtcctttt
     1681 ctcgttcgcc tgtccgggcg tggatcacgc tgatgctgtc gttgctaccg ttcgagacgt
     1741 agacgtgcga tcgggtggcg gcgattgagt tggggctgct tccccccacg gcgggaaagt
     1801 cttcgagcaa ttcacccacc aggtggccgg tcttggtttt tttgacgact ttttctttac
     1861 cggaccggga aatgcggatt ttccagaccg acatgcccgc aatatcgttc ggatcgccga
     1921 ggcccgatac cacaactccg tcgatggtga ctccttcctc ggcttctttg gaaggcaccc
     1981 cgaatggagg aaagggaagt ttggcatatt caccgtcttc cgtgcggaaa taggaatatt
     2041 catacattcc gacgttggct acgtaggcat ggtctccctt gggggagagg gcgatgccga
     2101 aggggtagcg gcccacgggt atcgagcgta cgaccgcgcg ctgggcggta tcaaggatca
     2161 gcatccggaa attggactgg tcgcagacgt agagcgtctt gccgtcgtgg ctcaatcgca
     2221 agtctccagc gtagccatgc cggaaattct gcccgtcggc gtcgtgggcg caatcgatac
     2281 ggaacagatg ctcacgcgtc tccaggtcaa acgcggtaac actccagtct ttgccgccgg
     2341 ccacatacag cgttttgttg tcgggcgcga tggccaggcc catgaatgcg gcatccagca
     2401 cgccttctcc ctcattctgg tcgttgggga tgaaaaagat ctccggcgcg ctggggtctt
     2461 tcatatccag gatggacagc tggggaccgt cgctgctgac ggcgacaacc cagcggccat
     2521 ctttgctcat ggtcaggcca tagggatgcg gacgcacccg gaacatggct ccgtcagggg
     2581 taatggtgcg cccattgggc aggattgagg tgccgccggg gtcctgggtg ggatactggt
     2641 cccgggctgg cgcccacatg accatgtccg acgcacccgc ggcgcaggcc gcggccacga
     2701 gagtcagtac cgtgactatc gtttgaagac atttcatggc atctacctcg aagattcgtc
     2761 cgggagaaga tcgcgttgat cacgcggcca ctcccaccag ccttatccat ggattgacaa
     2821 aacggcttgt gatgcactgt ccctgttttt gcaggccgtg actcctcaca ggtttgttat
     2881 aggccgatgt tttcttaaat acaagtctga agaaaattca acggagttca ttcgaatcag
     2941 cgcacggcaa tggattcaag ccgtgcactt tcgtttcgct ttagaaaggc ggccctcggc
     3001 agaggtttaa cgtgaatcga aaacctggcg aaacgagtga caatatcaac cttcaagaag
     3061 aggtgaaatg ttcgcgtgtc aaggtccggg caggcgcgcc ggtctggaat gaaaacttga
     3121 gccggcgaaa cttgcatcac ctccctgaga tgggtatttt gcgggaaggt gccgtgcgcg
     3181 gttgcgtttg cggtctggtg tcagcagtgc gtggatactg ggctccacgc tcaaggaggg
     3241 atgggcgatg cgcactcgga cgttgattgt atgtgtggcg gtggtgctgg ttggctgtgc
     3301 aacgcacgag cttccaggag tgcaacgcgc gctggacgcg ggaccggatg cgtttctcga
     3361 tcaagcggtc gtcgggccgc agggggatgg cacttttgtg gtgcccacga cacagcttct
     3421 gaaccccgcg ggggataccg tggagttccc cgggcggcca ctgggtattg ctgtacaccc
     3481 cgacggccgg ctgctcgccg ttaagaaccg gacagacgtc gtcttctttg acgccgtgga
     3541 tcggcgcgtg gtccagacgc tccccctgcc gaagggtggc agctcgttct gcggcatcgc
     3601 ctggagcgcc gatggaaacc gcgtgtggac caccgcggct caggcggcta tccactgcgc
     3661 cgagcggggc ggcggcggcg catttgaatg ggtcaagacc atcaacctgc ctggcccgga
     3721 cggcaaaggc gcttccgcgc ctggcggatt tgctctggac gaaaggggcg gtctgatcta
     3781 tgtggcgctc agccggaaca acagcgtcgg agtggtcgac atctcttcgg aggccgtgtt
     3841 gaccgagatc cctgtgggta tggcgcccta cgatgttctt cttcatggag gcaaggcgta
     3901 tgtgaccaac tggggcgggc ggcgccccac gcccgacgat attacggggc cgtcttctgg
     3961 cagtcaggtg gtgacaaccc cggcgggcat tgcatcgacg gggacagtgt ctgttctcga
     4021 ccttgaagcc agacgcacca gccaggagat tgaagtcgat ctgcatccct gcgggatggc
     4081 gctctcacca gacggcgcca cgctgtatgt cgccaatgca aacagcgata ccatcagcgt
     4141 gattgatacg gcggaccatg tggtgaaagg gcgctggatt gcccgtccgt cgctcgaact
     4201 tccctttggc agcgcaccca atgccattgc gctgcaggac aatggcgaat cgctgtttgt
     4261 cgcgctgggc ggcaacaact gcatcgctgt tatgagcgcc agagacgggc gcgtcactgg
     4321 gctgattccg acgggctggt acccgggagc tgtggcgttc gcacaggacg gcgccctgct
     4381 ctgcgtggcc aatacgaagg gcgccggaag ccggtatctg gaggagaaca ccacgcgtcc
     4441 agaaagcgag cgctatcctg gtgtgcgcgg ccgcagttcg cacgatcatc tgggttcggt
     4501 caccatagtg aggacaccga acgaaagcac gctcgatgcc tatacacatc aggcggctgt
     4561 agcgatgcgt ttgccacaca tgctgcgcga tgccggcgcg gccgagcacc ccaagcgtgt
     4621 cgtggcgatc ccggaacgcc ccggggatgt ctcgcccatc aagcacgtgc tgtacatcat
     4681 caaagagaac cgcacctacg atcaggtatt tggcgatatg ccgcagggca atggcgatcc
     4741 cacgttttgc catttcggac gcgaggtcac gcccaaccat cacgccctgg ctgaggagtt
     4801 tgtgctgctt gacaatttct actgcaatgg cgttttgagc gccgatggcc accagtggac
     4861 cgacgaaggc tatgtgaccg attatcttga gaaggctttt ggcggctggt cgcggagtta
     4921 tccgtacgat ggcggcgacg ccctggccta tgcgtcaaca gggttcatct gggatcatgt
     4981 tcttgccaaa ggcctgacat ttcgcaacta cggtgagatg gtcaagccgc ggatcacgcc
     5041 caatgatgcg acctgggccg acatctatca ggattatctg aacggaaccg ccacggtcaa
     5101 gattgaggcc tacactgagg tcaggggcct cgaagggtat ctctgcccca cgtatatcgg
     5161 atttccaggg aaggtgcagg atgtgtaccg ggttcgcgag tttctcaagg aatttcgtgc
     5221 ctgcgaggaa aagggcgaat ggcacaactt tgtgatcatg ctgctgccca atgatcacac
     5281 cgtgggcaca cgggaagggt ttcccacgcc gaacgcagcg gtcgccgaca acgatctcgc
     5341 gctgggtcag ctcgtggaag ccgtgagcca cagccagttc tggcccgaga cagccatttt
     5401 cgtcgtggaa gacgatccgc aggcaggact ggaccacgtc gacggacacc gcaccgttgc
     5461 gttgtgcatc agtccgtaca cccggcgcgg ggaagtggtc agcacgcact acaatcaaag
     5521 cagcatgctc cggaccatgg agctgatcat gggcctgccg ccgatgagcc agttcgacat
     5581 ggcggccaat cccatgtacg cctgtttcac aaacacgcct gatttgaccc cgtacacctg
     5641 ccgccccaac cagattcctc tggatgagat gaatccgaag ctggcctcgt tgcgtggaaa
     5701 gcagttgtat tacgcgcaga aatccatgga aatgcctctg gatgacattg atcaggcgga
     5761 cgaagatctg ttcaaccgaa tcctctggca ctccgtcaag ggatatgacg taccctatcc
     5821 gaagctcgca cggcgagcgt cttccgaggc ggttgtgtgg ggtttgaagg attaagatta
     5881 cgaaggacga tgaaccaggg taaagcataa gaagaggagc acaaatgcat aagaagacgg
     5941 gattcacgtt gattgaactg ctggtggtga tcgccatcat cggcatcctt gcggccatcc
     6001 tgctgccggc actgagccgg gcccgggaag ccgcgcgccg tgccgcgtgc gccaataacc
     6061 tcaaacaaat gggtttggcg ctctacatgt atgcggatga gcatcgcggc cgcttcccac
     6121 accggcaggt ctggacggtg acgggcggat tgagccgcga aatgatgttt aatggccccg
     6181 ccatgattcc tgaatacatc agcgatgtga acgtggtgtg gtgcccgtcg tggaacaagc
     6241 cgccggacat tgtgcttcgc tacgacggcg gcaaaggcaa tggcgatggc attatccagc
     6301 ccgaggaagt gggtcaggaa ccttaccact acaccggctg gctgatcatg gatgatgtca
     6361 acatcctcgg acccctggtg ggggtcatag gaaatggcat taacggacgc cacgatgaaa
     6421 acgaatacgt tgtaacgcct tggggcgaac tggcacaggc caatgtcgat accggcggcg
     6481 ccgccagcga cagcgatttt acggtttctg caacacatgc cggtacacag gtaggtggag
     6541 gcagtaccta ctaccggctc aggcagggca tcgagcggtt tatggtcact gatatcaaca
     6601 atccagcctc gagtgcggca gcctgcagca ttgttccttt gatgtgggat cacgtcagca
     6661 ccaatcccaa agacttctcc cacatccccg gcggcggcaa tgtgctctat cttgacggcc
     6721 atgttgagtt tctgcgctat cccagtgatc ggttcccgtt cactccagac agcgcgcgca
     6781 cgctgggaaa gtacgggaaa atatttgacg gcatagactg agcggccgga gtcagtgcgg
     6841 ctcaaatcca atctggaccg ttttggcgat agaaaaaggg ggcaaaggga cgcgtatgcc
     6901 cgatcgagtc attattggga gcgtgagcca ctgtggatca acgcatgaat ccggcagccc
     6961 tgccgcatga gagggccatg tggtaaaaca ggcaatatca aagacttacg gtgcttcact
     7021 cctggatggg agaatggtga tctggtggcc tcaactggaa ggatggcgag cctgcggcgc
     7081 tgcgccgggg ccgcttgcca tatagatggg gtgtgtaccg gttttttcag atgacattgg
     7141 gccgctgcat cgcatacgat gtgggctcct gatggttcgc ttgtcattac ttaagtggtt
     7201 caagtaaatt tgaccttatg cagcacggag aaatgccccg gcgagcgtcg cgcggcaaag
     7261 atgagagcac tcgtatcctt tttcagcaca acctgaacgt caagagtttg caggcatggc
     7321 cggattgaag acttccgaga cacagtgggc gcgtccctgg cttatctgga cggtcgtgct
     7381 gtttgtcctg gcgggcgcgg ttctggaatt cgcttccctt tacgcggatg cgaaccaggt
     7441 gcctccgcgg aacctgggta attttctcag tctggtgtcg gccgtttttc ttgcttccgc
     7501 gggcgttgta ctcgtgcgcc acctgaaata tgagcgtgca tcccgcacca tcatggaagt
     7561 ggcagcgggc ctgttcattt attccaaagt gatcgcctgg gcggggcgac ttccaacgct
     7621 gagcaccgtg ccgctgttcg gcatccatgg gccaggttat gaggtgcttg aatcgaatac
     7681 gcacacgatt gccttgcttc tggtcttgat tgggttcatc cgttcgatgt gggtagccaa
     7741 caagacgcgc cacgacctgg aagtccagaa tcaggccctg ctgcacgaag tggaacagcg
     7801 cacatcggtt gagcgcgagc tgcgcgccag tgaaatccgg taccgcgacc tggtggcgga
     7861 aatccccgac gtagtctggc gtctggatac gaccggcgtc ttcaccttcg ccagcccggc
     7921 ggttgagatg gtgtttggca tgcggccgga tgcggttgtg ggcaagcacc tggctgatgt
     7981 acttcctccc gcgctggcag aacgtgcgct ggcctctctg acaaagaaga tacagaccga
     8041 gcccggggaa gctcttgtac cgttccagtt tcatcaaagc ctgccggatg ggcgagtgcg
     8101 cttcttcgag gccatgagcg cccccgtata cagcctggaa gggcagattg aggaaataca
     8161 ggggattttg cgcgatatca ccgcccatat tgaggccaac gaagcgctgg cagccagtaa
     8221 agagagatac cgggcgtttg tcgaaaacag ccccatggtg attgtccgga ccgatcgcaa
     8281 tctgaagtgc atttttgcca accgggcctc acttgacatc gccgggctga cgcgtgatga
     8341 agtgctcgga aaaggccagg aagccattca agatgtcatg caccccgatg accatgcctt
     8401 gattgtcacc cattgtcatg aagtgctcgc taccgaagaa cgccgtgcga ccgaggtccg
     8461 gtttcgcgat ccgtcaggcc agtggcgctg gttcgcccat ctcgtctatc cgtggtatga
     8521 gccgaacggc cagctggggg ggatcgaggc gtcggcgcgc gatgtgactg agcagaagcg
     8581 ggatcaggaa gcgctccggc agagtgaaga aacggcccgg gcgctcctga acgcgaccag
     8641 cgacctcgtc gggcttcttg atgaggacgg ccggctgctc gcctgcaatg aacattttgc
     8701 ggccagtgtg ggtataccgg catcggagct ggtgggcgcc aattaccgcg aactggttca
     8761 gggcgatacc ggccgtaagc gatgggaaaa tgccgtggcc gtgatgcgaa ccggccggca
     8821 gatcttattc agcgacgatt ataacggccg gcatttcgag cactgcttct accccgtgag
     8881 ggatgaacaa ggcaagtgta cacggcttgc cttctactcg cgcgacgtga cgaccttcct
     8941 tcgcaaggaa gaggaacgca tccggctggc cgcggccatt gaacaggcgc cggacctggt
     9001 catcatcacg gatgccgatg gctttgtgca gtacgtgaac cccggtttcg agcgcaacac
     9061 ggggtatgcg cgcgaggacg ttgccgggaa gaccacgcgg tttctccgtt cgagcgaaca
     9121 cgacgatgca ttttatgagg cgatccaggg aacgatttcc cggggagtga cgtggtcggg
     9181 aaggatggtg tgccgccgga aagacgagag cctgttcgaa tcagacgcga ctgtctcgcc
     9241 cgtgcagaac gccgcaggcg tgatcagtaa ttacatctgg ctgatgcatg acgtgtcgac
     9301 cgaggtaagc ctcgaacgcc agttgcgcca ggcgcagaag atggaggcgg tgggcacatt
     9361 ggcggggggc atcgcgcacg atttcaacaa cattctctcg ctgatcctcg ggtacagtga
     9421 gttgctgctt gatctcctgg cagacaacga gccggcgctg ctgaacgtgc ggcaaatcgc
     9481 caaagccggc aaccgcggcg ctgagctggt gcggcagatc cttacattca gccggcaggc
     9541 ggagcggcgt tgcgagccct gcaggctcgg tccgatcctg aaggaatcgc tccgctttct
     9601 ggcggcgtcc ctgccggccg ctgtggagat tcagcagcgt atcggggagg aaaccggctc
     9661 ggtcatggcc gacccgatac agatttatca gacgataatg aatctctgca ccaacgcgta
     9721 tcaggcgatg ggcaacggag gcggcgtgct cgaactgacg cttgacgaag tgaaaatcga
     9781 tgcgtcgttt cacgccgatg ccggctcgcc cgaaccgggc aaatatgtgc ggctgaccgt
     9841 gtccgatacg ggtacaggga ttgagccggg cgatattggg cgtatattcg agccattttt
     9901 ctccaccaaa aaacccagcc agggaaccgg actcgggttg tctaccgtgc atggtattgt
     9961 gaccggatat ggaggcgcgg tgacggttcg cagcgtggtt ggcgaaggta cagtattcga
    10021 ggtgtacctg cctcaaatgg acgcagaagc cccggtggaa cgcgtgagtg aggagtatcc
    10081 tggggcggga accgagcgca tcctgtttgt ggatgacgat ccggacctgt ctcagatggc
    10141 ggggatgatg ctgcagcagc tcggctaccg cgtcgagacg ttcaccaaca gtctggcggc
    10201 gttcgaggca tttcgggcac ggcctgtcaa gccatttgct aatcttaccg gggcgtatat
    10261 cgtcgcgcca tttgg
//
LOCUS       DAOPBQ010000056        10041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6459_length_10041_cov_4.220909, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000056 DAOPBQ010000000
VERSION     DAOPBQ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10041
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6459_length_10041_cov_4.220909"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PKW60_03120"
     CDS             <1..637
                     /locus_tag="PKW60_03120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPJ98454.1"
                     /translation="GLTDCEYECLFVNDGSTDGTRAELGRLAEANPRVRALHLVANRG
                     QSAALVAGIRRARGAYIFILDGDLQNDPCDFPRMLELLQDYDCVCGYRAHRQDSWIRR
                     LSSRLANRTRALFVPTGTRDAGCGSKGFRRVCAPHIMSFNGAHRFLDSMLVNAGFTVT
                     ECPVTHHPRQHGVSKYGIHNRLWRGLYDLAGVRWLCKRYVRFEVEGEDGHD"
     gene            630..1322
                     /locus_tag="PKW60_03125"
     CDS             630..1322
                     /locus_tag="PKW60_03125"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HPJ98455.1"
                     /translation="MTDPAAAEPSLLWYALGVPASVIFYGRFYVQWIASELRRKSIVP
                     VSFWYMSSLGSLMLLVYGVATTSALGTLSHCFNIAVYSRNLIHIWREKGRLSFGLNLG
                     VHVFLAVVILASVAVTAWTWWHVYDRSSAGPGGELRKTALWLGVGVLGQILFALRFLV
                     QWVATERSRKSVVPTSFWWLSLVASALMCASFTREREWVYAVGIVATLFIYARNLWLI
                     YVYGNEGADAGD"
     gene            1573..2139
                     /locus_tag="PKW60_03130"
     CDS             1573..2139
                     /locus_tag="PKW60_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPJ98456.1"
                     /translation="MKFVTLMGSPRKEGNTATVLAWAEEELKARGHEIERINIIDRSI
                     GYCDGCFRCQDAEGGTGCPMEDDAGVIFKRMIEADGFILASPLYCWGFSAPLKALLDR
                     GVCLVKRPADGDPIYLLERKRAGLLVTAGGPDDGNADLITEVFNRFTEYLRCTRAGRL
                     VVPYCRLNMEFGPNVEVQARDFAQDMVE"
     gene            2150..3427
                     /locus_tag="PKW60_03135"
     CDS             2150..3427
                     /locus_tag="PKW60_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98457.1"
                     /translation="MRKRAYVPRVAGMIMAGLALVLAGSGAGAGEEPAAETPKMQANV
                     PIRAITSGPQAHWFAYYDKWQFDINNRYVLGNQVAFEDRAPEASDVIQLGMVDTEDGD
                     TWIPLAETSAWCWQQGCMLQWLPGSESEVIYNIRTEVGYASVIQDVFTGEKRRLSHPI
                     YTVSGDGKYAVSLNFSRVAWTRPGYGYNGFPDRNEESLYPGDDGIYWIDLATGESKLI
                     ISLADIVQIAPDETMDKGKHWFNHLLFNQDGSRFIFLHRWHKGKGPGRFTRGFTARPD
                     GSDIFCFGEHGMVSHFIWRDPKHILAWSTEPAGNFFHLYEDQAKTVETVGAGVLTVDG
                     HCTYSPDGHWILTDTYPGRERMQNLVLFRPEDGTLVSLGRFYQEQPTDQEWRCDLHPR
                     WSRDGRSVCIDSKCSGQRQMYLLDVSGIILPSE"
     gene            3473..5224
                     /locus_tag="PKW60_03140"
     CDS             3473..5224
                     /locus_tag="PKW60_03140"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HPJ98458.1"
                     /translation="MLGYAGTAQAGMERHEPGRIRAVEDLKVYRTISKRVGNAVRLAE
                     VVQVLVRYGFSDVVHRLSLHEGLPAKVLRGLRLIEAPSGEPETQGRRLREALTELGPT
                     AVKFGQILSTRPALVGAELAKDLGQLQDHVHALEFDQLKPVIEEELGAPIADLFSEFN
                     EEAVASASLSQVYRARTRTGNQVAVKVQRPGIRRIIEADLLLMHAIAEWIAERVPDIA
                     WMNPAGTVDEFARSVRRELDFNIEARIIEQFRKNYDGDPQVFVPRVYSELSSERVLTM
                     DWVEGVRVDDIAQYPQYRSNPHTVAATGADTLCKQVFEYRFFHADPHPGNILLIGENR
                     IAFLDYGMVGSLEEADTATIADVLRAIFRQDAESAVQAMLQFTTSGEADDYSALVHEV
                     ADYIAFDAQAILSTGEVGKAIDRLTTIMRKYRLQLAPRVSLLMKALVTVESTGRALDP
                     GIDMVPIIKPYVERIVAHRFSPAQLARETRHNMALFVRMGRQMPGDLSHLLRMLRAGK
                     LKIQLNHEGITHLAAAMDRASNRVTVGLITGAVIVGSSLLLGADIGARTLGLSGFIFA
                     GILGLSLVVSILRSRNI"
     gene            5241..6119
                     /gene="ispE"
                     /locus_tag="PKW60_03145"
     CDS             5241..6119
                     /gene="ispE"
                     /locus_tag="PKW60_03145"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPJ98459.1"
                     /translation="MAHTLTLQSHAKINFYLDVLSRRPDGFHDIETIFQTVSLADTLQ
                     FAGAASGITLTCSRADLDCGPRNLVCRAAALLQARAGQTRGAQIHLEKNIPVAAGLAG
                     GSGNAAATLVGLTELWDLSFSPAELQAFALELGSDVPYCLIGGTQEATGRGERLSALP
                     PVPETWCVLVHPPIQVSTAAVFNSPRLLRNTEKPVAGRTSAFRRALDALRAGDWAQAV
                     FNRLETPVFEEHPGLETIKSRLLEAGCIAAAMSGSGPTVFGVCSSRALAERVAAALAP
                     SAVSVVKTVDSALRAV"
     gene            6163..8508
                     /locus_tag="PKW60_03150"
     CDS             6163..8508
                     /locus_tag="PKW60_03150"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HPJ98460.1"
                     /translation="MNATRVLMGCAVLGLAAVMTAAEEGGSQPIAYYDMTGLNRADLK
                     DPVQARRAWDTAHLVASVQGIVNRDGPRLFIRFMEQPDDFWFDYCRGESGWLTERPVE
                     TLPSLEALLRRFGGALTGLVVYDERVPATSNLASTIVGIENRACLRYDPSAGSLYTAV
                     LAMDLPFTRDITRLIRDDGSPLFTGEGLIPDTDRPSTGSAKCDAYLWAKNRYLDTGAC
                     SREFMAYYIDAYWLTAPTLNSLPNNTLTNHDFFIANKAFFFDLHVWEEESPVDDPGQE
                     PGTDTATLHQLLKSMYAQAGGRIIHIGGFVPWAWKYTNHGNAGSQHGPVDSEWEYARI
                     ISAYNGIMDADALGLAGMANASFYRHFPLRERYPQNPKPTAASLRERGLLLESGAPAE
                     KTYICFYMGDYDAASWFNRHVPKWWQDPAHGQTLCTWAFNPNLDRRAPHAMHYIRTHQ
                     TANDWFMFGDSGAGYLNPGMLLEEHRRGVSGLPSGLDAWVAHNRPYAERYDLSINGFI
                     IDGHSPGMGDEGMDAYLTFAPDGLVGQKIPVQGVFRDTLPYVRMKLDLDGSPADAGAT
                     LASLAGVNAPKFLFIRTILKSPSWHRDTMSAAMAIDSSLTFVDPYTFFLLLKAHEQAR
                     EDVALTVMTEAEYVAGASIHGLAPVRVGDGPFALETAGEEMVLRQAGAEQTRYVYFEF
                     ADAFAVPFQPAPGKTAVVTATVLDSAPGEIGIHYDSHDDGAYRDGPRHTLTGSGKWVE
                     LTFELPNAAFTHRQNGGADFRLVNYHNNLVLRGVRVTSGGG"
     gene            complement(8613..9191)
                     /locus_tag="PKW60_03155"
     CDS             complement(8613..9191)
                     /locus_tag="PKW60_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPJ98461.1"
                     /translation="MLRDLILRSRSIRRFHEDTPVNPKALRELVDMARLTPSGANLQP
                     LKYVTSTSKELNEHIFSTLAWAGYLKDWEGPVPGERPGGYIVILGDRGVAPSFGCDHG
                     IAAQTICLGAAELGLGACIVGSIERNELLAILELPVSQYELLLVIALGKPRERIELEL
                     VAEPGKIEYYRDENDVHHVPKRRLEDVLIAEH"
     gene            9703..9778
                     /locus_tag="PKW60_03160"
     tRNA            9703..9778
                     /locus_tag="PKW60_03160"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9736..9738,aa:Lys,seq:ttt)
     gene            9819..9904
                     /locus_tag="PKW60_03165"
     tRNA            9819..9904
                     /locus_tag="PKW60_03165"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9853..9855,aa:Tyr,seq:gta)
BASE COUNT         1856 a         3134 c         3114 g         1937 t
ORIGIN      
        1 cgggttgacg gactgcgagt acgagtgcct ctttgtcaac gacggcagca ccgatggcac
       61 ccgcgctgaa ctgggccggc tggcggaagc gaacccgcgc gttcgggcct tgcacctggt
      121 ggcgaaccgg ggccagtcgg ccgcgctggt ggccgggatt cgccgcgccc ggggcgcgta
      181 catctttatt ctcgacggcg acctccagaa tgatccctgc gatttcccca gaatgctcga
      241 actgcttcag gactacgatt gcgtgtgcgg gtaccgggcc catcgtcagg attcgtggat
      301 ccggcgcttg tcgagcaggc tcgctaaccg gacacgggct ctgttcgtgc cgacggggac
      361 gcgcgacgcc ggctgcggca gcaaggggtt tcggcgggta tgcgcgccac atattatgtc
      421 cttcaacggc gcgcaccgct ttctcgattc gatgctcgtc aatgcgggct tcaccgtgac
      481 cgaatgcccg gtgacgcatc atccccggca gcacggcgtg tcgaagtatg gcatccacaa
      541 ccggctctgg cggggcctct acgatcttgc cggagtacgc tggctctgca aacggtatgt
      601 gaggttcgaa gtggaagggg aagacgggca tgactgaccc cgccgccgcc gaaccttcac
      661 tgctctggta tgccctgggc gttcccgcga gcgtgatctt ctacggccgt ttctacgtgc
      721 agtggatcgc ctcggaactt cggcgcaaga gcatcgtgcc cgtttcgttc tggtacatga
      781 gcagcctggg ctcgctcatg ctcctggtgt atggcgtagc caccacctcc gccctgggca
      841 cgctgagcca ctgtttcaat atcgccgtgt acagccgcaa tctgatccac atctggcgtg
      901 agaagggcag attgtcgttc gggctcaacc tgggcgtgca cgtctttctc gccgtggtca
      961 tcctggcgtc cgtggccgta accgcgtgga cctggtggca tgtctatgat cgcagcagcg
     1021 cggggccggg cggcgaattg cgcaagacgg ccctgtggct gggcgtgggg gtactgggac
     1081 agatcctgtt cgccctgcgc ttcctcgtgc aatgggtggc cacggaacgc tccagaaaga
     1141 gcgtggtgcc aacgtcattc tggtggttga gcctggtggc gtcggcgctc atgtgcgcga
     1201 gcttcacgcg cgagcgtgaa tgggtgtatg ccgtgggaat cgttgcgacc ctgttcatct
     1261 atgcccgcaa cctctggctc atttatgtct acgggaatga aggcgcagac gccggcgatt
     1321 gacgcaaatt ctttcttctc cgtccggtat ttgttagggt acagggcgaa tctcgcgaca
     1381 ggatagtccg ggacggattt tccgtttccc atggaaatgg ccacacagca cgcgaggcgt
     1441 atggaggagt gctccatatg ccgcccgcgg gttttcgaga ctgattcctg aggcaggcag
     1501 atcaaacgcg gctggacacg ggagcggcag cgccgcccgg atccaggcgc ggattacgga
     1561 aaggccacac acatgaagtt tgtgacactg atggggagtc cgcgtaaaga aggaaatacc
     1621 gccacggtgc tggcctgggc ggaggaggaa ctgaaggcgc gggggcacga gatcgagcgc
     1681 atcaacatca ttgatcgctc gatcggatac tgcgacggct gtttccgatg ccaggacgcg
     1741 gaagggggta cgggatgccc catggaagat gacgccggcg ttatcttcaa gcgcatgatc
     1801 gaggcggatg gatttattct cgcctcgccc ctgtattgct gggggttttc cgctccgctg
     1861 aaggcgctgc ttgaccgcgg cgtatgcctg gtgaagcgcc cggcggatgg cgatccgata
     1921 tacctgttag agcggaagcg cgcgggcctg ctggtgaccg cggggggacc ggacgacggc
     1981 aacgccgact tgatcacgga agtgttcaac cggttcacgg aatacctgcg ctgcacacgc
     2041 gcaggccgtc tggtggtgcc ctattgccgc ctgaacatgg agttcgggcc gaacgtggag
     2101 gtgcaggcgc gcgattttgc gcaagatatg gtggaataga ggggaagcta tgcgaaagag
     2161 agcctacgtg ccgcgggttg ctgggatgat catggcgggt cttgcgctgg tgctggctgg
     2221 cagcggggcc ggtgcaggcg aagagcccgc agcggagacg ccgaaaatgc aggccaatgt
     2281 gccgatacgc gcgatcacat cggggccgca ggcgcactgg ttcgcctatt acgacaagtg
     2341 gcagttcgac atcaacaacc gctacgtgct cggcaaccag gtcgcctttg aagaccgtgc
     2401 gcccgaagcc agtgatgtga tccagttggg catggtcgat accgaagacg gcgacacctg
     2461 gatcccgcta gcggaaacct ccgcctggtg ctggcaacag ggctgcatgc tccaatggct
     2521 tcccggctcc gagagcgagg tcatctacaa catccgcact gaagtcggct atgcgtcggt
     2581 gattcaagac gtgttcacgg gcgaaaaacg ccgcctgtcc catcccatat acaccgtaag
     2641 cggcgatgga aaatatgccg tcagcctcaa cttttcgcgc gtcgcctgga ctcgccccgg
     2701 ctacgggtac aacgggttcc ccgatcgcaa cgaagaaagc ctgtatcccg gggacgacgg
     2761 catttactgg atcgatctcg ccacgggcga atcgaaactc atcatttcgc tggcggatat
     2821 tgtgcagatt gcgcccgatg agaccatgga caaaggaaaa cactggttca accacctgtt
     2881 gttcaatcaa gacggttcgc gcttcatctt tctccaccgc tggcacaaag gcaaggggcc
     2941 tggccgtttc acgcgcgggt tcacggcgcg ccctgacggc agcgatatct tctgtttcgg
     3001 cgaacacggg atggtctctc atttcatctg gcgcgatccc aaacacattc tcgcatggtc
     3061 gaccgaaccc gcgggcaact tctttcatct ctatgaagat caggccaaaa cggtcgaaac
     3121 cgtcggagcg ggcgtcctga cggtcgacgg gcattgcacc tattcgccgg atgggcactg
     3181 gatcctcacc gacacctatc ccggccgcga gcggatgcag aatctcgtgc tgttccgccc
     3241 ggaggatggc acactggtga gcctgggccg cttctaccag gagcaaccca cggaccagga
     3301 gtggcggtgc gacctgcatc cgcgatggag ccgcgacggg cgctccgtct gcatcgactc
     3361 gaagtgttcc ggccagcgcc agatgtacct gttggatgta agcgggatca tcctgccgtc
     3421 ggagtaggcg cgccctgcac aggccggcgt cttgcgcttg gttttttcct ctgtgcttgg
     3481 ctatgctgga accgcgcagg ccgggatgga gcggcacgaa cccggccgga tccgggccgt
     3541 cgaggacctc aaggtgtacc gaaccatctc aaaacgcgtc ggaaatgccg tgcggctggc
     3601 tgaagtcgta caggtgcttg ttcgctatgg attctccgac gtagtccatc gcctgagcct
     3661 gcacgaaggg ttgccggcaa aggtcctgcg gggattgcgg ctcatcgagg cgccctccgg
     3721 tgaacccgag acccagggcc ggcgcctgcg ggaggcattg accgagctgg ggcccaccgc
     3781 ggtgaaattc ggccagatcc tcagcacccg ccccgcgctg gtcggcgccg agctggcgaa
     3841 agacctcggg cagcttcagg accatgtaca tgccctcgag tttgaccagc tcaagcccgt
     3901 gatcgaggaa gagcttggcg caccaatcgc ggacctcttt tccgagttta atgaagaagc
     3961 ggtggcgtcg gcgtcactca gccaggtcta ccgggcccgc acccgcaccg gcaatcaggt
     4021 ggcggtgaaa gtgcagcgtc cggggatccg ccgaattatt gaggccgacc tgttgctgat
     4081 gcacgccatc gccgaatgga tcgccgagcg cgtgccggac attgcgtgga tgaaccccgc
     4141 gggaaccgtt gatgagttcg cgcggtcggt gcggcgcgag cttgatttca atatcgaagc
     4201 acgcatcatc gaacaattcc gcaagaacta cgatggcgac ccgcaggttt tcgttccgcg
     4261 ggtctattct gagctctcat ccgaacgcgt gctgactatg gactgggtgg aaggcgttcg
     4321 agtggacgat atcgcgcagt acccccagta tcgctccaac ccgcacaccg tagccgccac
     4381 cggcgccgat acgctgtgca aacaggtctt tgaataccgg tttttccatg cggatcccca
     4441 tcccggcaat attctgctca tcggggagaa ccgcatcgca tttctcgatt acggcatggt
     4501 gggcagtctc gaagaagccg acacagcgac cattgccgac gtattgcggg cgattttccg
     4561 acaggacgcc gaatcggccg ttcaggccat gctccagttc accacgtcgg gcgaggccga
     4621 tgactatagc gcgctcgtgc acgaagtggc cgactacatc gcgtttgatg cgcaggccat
     4681 cctgtctacg ggcgaggtcg gcaaagccat cgaccggctc acgaccatca tgcggaaata
     4741 ccgtcttcaa ctcgcgcccc gcgtctcgct gctcatgaaa gcgctggtca cggtggaatc
     4801 cacgggtcgc gcgctcgatc ccggcatcga catggtgccg atcatcaaac cgtatgttga
     4861 gcgcatcgtg gcgcaccggt tctcgcccgc gcagctggcc cgcgaaaccc gtcacaacat
     4921 ggcgctcttc gtgcggatgg ggcgccagat gccgggcgac ctgagccatc tcctgcgcat
     4981 gttgcgcgcc ggcaaactca agatccagct caaccacgaa ggcatcaccc atctcgccgc
     5041 tgccatggac cgggcaagca accgcgtcac cgtgggcctg atcaccggtg cggtcatcgt
     5101 gggttcaagc ctgttgctgg gcgcagacat cggtgcgcgc accctgggtc tcagcggctt
     5161 catctttgcg ggcatcctcg gcctctcgct ggtggtctcc attctccgat cgcggaatat
     5221 ctgaccggat ggatgcgccc atggcccata cactgaccct ccagtcgcac gcgaagatca
     5281 atttttacct ggacgtcctc agccggcggc cggacggatt ccacgacatc gagaccattt
     5341 tccagaccgt ttcactggcc gataccctgc aatttgccgg ggcggcgtcc ggcatcacgc
     5401 tgacctgttc ccgagcggac ctggactgcg gaccccgcaa cctcgtctgc cgggcggccg
     5461 cgctgctgca ggcccgtgcc ggccagaccc gcggcgcgca gatccatctc gaaaagaata
     5521 ttccggtggc ggcgggcctg gcgggcggtt ccggcaatgc tgcggcaacc ctcgtaggcc
     5581 ttactgaact ctgggatctg tcgttctcgc ctgcggagct gcaggccttc gcgctcgaac
     5641 tgggctccga cgtgccctac tgcctcattg gcggaacgca ggaagcgacg gggcgcggcg
     5701 agcgtctcag cgccctgccg cctgtgcccg aaacctggtg cgtcctcgtc catccgccaa
     5761 tacaggtgag caccgctgcc gtgttcaaca gcccgcgatt gctccgcaac accgaaaaac
     5821 cagtcgccgg gcgtaccagc gcgtttcgcc gggccctgga tgccctgcgc gcgggcgatt
     5881 gggcgcaggc ggtcttcaat cgcctggaga cgccggtatt tgaagagcat cccggcctgg
     5941 aaaccatcaa atcccgtctg ctcgaggccg gttgcattgc cgccgccatg agcgggagcg
     6001 gtccgaccgt ctttggcgtc tgtagcagcc gcgccctggc ggaacgcgta gccgcagcgc
     6061 tcgcgcccag tgcggtcagt gtggtcaaaa ctgtagattc ggccttgcgt gccgtgtgat
     6121 gctcgcgcac ggcaactggc ccggttattg aaggaattgc tcatgaatgc gactcgtgtg
     6181 ctgatgggat gcgccgtatt gggtctggct gccgtcatga cggcggcaga agaaggcggt
     6241 tcgcagccga tagcatatta cgacatgacc gggctcaatc gcgccgacct gaaagatccg
     6301 gtgcaggccc ggcgggcatg ggataccgcc cacctggtcg catcggtaca aggcattgtc
     6361 aaccgggacg ggccacggct ctttatccgc tttatggagc agcccgacga tttctggttc
     6421 gattactgcc ggggcgaatc gggctggctc acggaaaggc ctgtcgagac cctgccgtcg
     6481 ctggaagccc tgttgcggag gtttggcggc gcgcttacag gcctcgtggt ctatgatgag
     6541 cgcgtaccgg ccacatccaa cctcgccagc accatcgtgg gcatcgaaaa ccgcgcctgt
     6601 ctgcggtacg acccgtctgc cggttcactc tacacggcgg ttctggcgat ggacctgccg
     6661 tttacgcgcg acatcacccg gctcatccgc gacgacggct cgcccctgtt cacaggcgag
     6721 ggccttattc ccgatacgga caggccttca accggcagtg cgaagtgcga cgcctatctc
     6781 tgggccaaaa accggtatct cgatacgggt gcctgcagcc gcgaatttat ggcctattac
     6841 atcgatgcct actggctgac cgcgccgacg ctgaattcat tgcccaacaa cacgctcacg
     6901 aaccacgatt tcttcatcgc aaacaaggca tttttctttg atctgcacgt atgggaagag
     6961 gaatcgcccg ttgacgaccc cggccaggag cccggcaccg acaccgcgac gctgcaccag
     7021 ctcctgaaat ccatgtacgc acaggccggg ggccgcatca tccacatcgg cggatttgtc
     7081 ccctgggcct ggaaatacac caatcacggc aacgcgggga gccagcacgg cccggtcgac
     7141 agcgaatggg aatacgcccg gatcatctcc gcctacaacg gcatcatgga tgccgacgcg
     7201 ctcgggctgg cgggcatggc caacgcgtcg ttctaccggc atttcccact gcgcgagcgc
     7261 tatccgcaaa acccgaagcc caccgcggcg agcctgcgcg agcgaggact gttgctcgag
     7321 tccggcgcgc ccgcggaaaa aacctacatc tgcttctaca tgggcgacta cgacgcggcc
     7381 tcgtggttca accggcacgt gcccaagtgg tggcaggacc ccgcccacgg ccagaccctc
     7441 tgcacctggg cctttaaccc gaatctggac cgccgcgcac cccacgccat gcactacatc
     7501 cggacgcatc agacggccaa cgactggttc atgttcggcg attcaggtgc gggctatctc
     7561 aatccgggca tgttgctcga agagcaccgg cgcggcgtgt cgggcctgcc gtcgggactc
     7621 gatgcctggg tcgcccacaa ccgcccatac gccgaacgct acgacctttc catcaacgga
     7681 ttcatcatcg acgggcattc gcccggcatg ggcgacgaag gcatggacgc ctatctcacc
     7741 ttcgcgcccg atgggctggt cggccagaag atcccggtgc agggcgtgtt tcgggacacc
     7801 ctgccgtatg tgcgcatgaa actcgacctc gacggctcac cagccgatgc cggcgccacc
     7861 ctggccagcc tggctggagt caacgcgcca aaatttctct ttatccgcac aatcctcaaa
     7921 tcgccgtcgt ggcaccgcga taccatgagc gccgccatgg ccatagattc cagcctcacc
     7981 ttcgtggatc cctacacctt cttcctcttg ttgaaagcac acgaacaagc cagggaggac
     8041 gtcgcgctga ccgtgatgac ggaggcggaa tacgttgccg gagcatctat ccacgggctc
     8101 gcacccgtgc gggtgggcga cggacccttt gcgctcgaga ctgcggggga agagatggtg
     8161 ctgcggcagg ccggagccga gcagacccgg tacgtctatt ttgagttcgc cgacgcattt
     8221 gccgtcccgt ttcagcctgc cccgggcaaa accgccgtgg tcactgcaac cgtgctcgac
     8281 agcgcgcccg gcgaaatcgg catccactac gattcccatg acgatggcgc ctaccgcgac
     8341 ggcccgcgcc acacgctcac gggctcaggc aaatgggtgg agctgacttt cgaactgccc
     8401 aatgccgcgt ttactcaccg ccagaacggc ggcgccgact tccgcctggt caattatcat
     8461 aacaacctgg tcctgcgcgg cgtccgggtc acgagcggcg gaggctgacg gcaatagagc
     8521 cgcccgcccc gtgtgctggt tccaaggagg ggagaaaggg ataaagcacg gctgccagtc
     8581 tcaaacgccc tggcccatga aatggtcgcg cactaatgtt ccgcgatcag gacatcttcg
     8641 aggcggcgtt tgggcacatg gtgaacgtca ttttcgtcgc ggtaatactc gatctttccg
     8701 ggttcagcca cgagttcaag ctcgatgcgt tcgcgcggtt ttcccagggc gattaccagc
     8761 aggagttcat actggcttac cgggagctcc aggatggcga gcagctcgtt gcgctcgatg
     8821 ctgcccacaa tgcacgcgcc gagccccagc tctgccgcac ccaggcaaat ggtctgtgcg
     8881 gcaatgccgt ggtcgcagcc aaacgacggc gcgacgcccc ggtcacccag aatgacgatg
     8941 tatccgccgg ggcgctcgcc cgggacgggg ccttcccagt ccttcaggta gccggcccag
     9001 gccagcgtgc tgaaaatgtg ctcgttcagc tctttcgacg tggaagtgac atatttcagc
     9061 ggttgcagat tggcgcccga aggcgtcaga cgcgccatat ccaccagttc ccgcaaggcc
     9121 ttcggattga cgggggtatc ctcatgaaac cgccggatac tccggcttcg caaaatcaaa
     9181 tcgcgcagca tgctgttctc caatggccgc gcggggctcg tccggcggcg aaatgctaat
     9241 taaagcgcga ttatgccgat cgggcaggcc tgcttcaagc atcgtacccg cggtgccggt
     9301 tgatcttcta aatgcgctgg ccctgttttt ctgagaaagg tggcccctgg acgggggaga
     9361 atgagcgcga atggacgcgc tgcctgggtc tcctgcgctt cattgggccg ggatttgcac
     9421 ctctacgccc gcgccttggg ctttcacagt gagtacacgt catgtcgcat cacagccgcc
     9481 gcatcaagat accagggtgc agcgtcaacc gttccaactc cttccccgct atgtacatgc
     9541 ccatgttttt cctcctgaac atggacaaag aggagatttt tactttgcgc agtgcggaca
     9601 ttatcaaatt gcgcctgcat agcgattccg gcgacttgac atgcttttcg gccgtttggt
     9661 atagtgcctc ttgccgcctt tcggcggcgt ttgttttttg gcgctggcgt agctcagttg
     9721 gtagagcaac ggacttttaa tccgtaggtc gggggttcga ctcccttcgc cagctccatt
     9781 gcgaaggaca gcgcggcgtg cgcgttcttg cggctcgtgg tgggctgccc gagtggctaa
     9841 aggggacggg ctgtaaaccc gttggcttgc gcctacgctg gttcgaatcc agcgcccacc
     9901 accacgatat acaggcttcg ccgcctggaa caggcgtgcg gagctggccg atagagttgc
     9961 gatcggatcg catcgatgag cgggaatagc tcagttggct agagcgtcag ccttccaagc
    10021 tgagggtcgc gggttcgaat c
//
LOCUS       DAOPBQ010000057         9941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6528_length_9941_cov_3.998179, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000057 DAOPBQ010000000
VERSION     DAOPBQ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6528_length_9941_cov_3.998179"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2261)
                     /locus_tag="PKW60_03170"
     CDS             complement(<1..2261)
                     /locus_tag="PKW60_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98462.1"
                     /translation="MNEITTPPRKSWPRRHKWLTTLAIVAVLLFVAIQAVERFIHIDR
                     YRSYVVQILEDHTGMPVSIDRLELTLFPQPSVAAYNVSIGEGELQAQCARARATATWN
                     GLTARQITVTDVSFDGLVASLPNEIAVIRDRIDYIQERMKAKPSPVSWKAAVDRIRAR
                     SARVSVSGFEQPVFEGDFEARDVLSDAIPVTFSGTLPFLGEPSRGEGELLFTVQKDAE
                     PLVIPSGWVEVKGIDPRHVLEREAMPPLVFEVRATFDEVTGELIGMKLAGEAVAARGA
                     PELVRAAAGPFTGMLWIRGSEITLNSLSLTAPSLTALADATRTADGAIACELQEVQVR
                     GDALAAVYAMVNSEHLQLTPQKDASITATNLLVGVDAEGALRLVKGQIQFAGVQPTLA
                     DGSKPFGDIHGQVHLDEGVIRVDKLEGSGLAVHGSVRPDLQRESAFVDLQAELTLSPG
                     VIAAVLKDDARIKGLSGRMAFKRIAGTFEPGKALPDDLKIDGALEKAQATLVLGELEE
                     TLSGISGRFAAEPGAIKVTGSCTSTLLGPITADARITPEKQRVTGVLQADLAQLNLPF
                     LNDETTRGAAMNIARVYGASAFDFEVALPAAAAPEGFVRIARQGTPELSATVALVHAK
                     GAISVSKADTRAAVPLAALRDLVPGSLHLEGTAAVSAAVPLNQDSFEAHVDLVQAALR
                     IGDYVNKRPGDPATADIRVARVDNSWKPQTATLHYGGEDFVARFEKDRIVSDFAFEVA
                     SLKPLFSGDVQANG"
     gene            complement(2610..3443)
                     /locus_tag="PKW60_03175"
     CDS             complement(2610..3443)
                     /locus_tag="PKW60_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPJ98463.1"
                     /translation="MFSSYINEGVPTAVFWLFVSAAIIIQGISKSGFAGGAGILSMPL
                     MFFVMPVNRAAAVMLPLLILCDMNAIYHHRHNRDWKTVLRLYLPSLLGILAGAAVWWW
                     IGREGVERYGSWIKRFVGVIAIVFAFYIISKEAAMSWVGRFRPGAGVGVAAGLAAGFA
                     STIAHAAGPIASLYIFAQDLGKTLFVGTVAWTFTLINLSKLPFYIAAGLIEKDVLLLG
                     AVLVPLIPAGSWLGKWMHHRVSEKVFNRIICVLVLIAGIQLIFGINPVSWALTLLRSG
                     A"
     gene            3978..4904
                     /locus_tag="PKW60_03180"
     CDS             3978..4904
                     /locus_tag="PKW60_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ98464.1"
                     /translation="MPRPLAKHKLVLAGLAPLALVCILAVSWYVTASPGDLEKLAPYA
                     EKYTLTKLLDTLQASGPAPETFSFVVLGDTRNNVFVASKIFEQAAQEAPTLAFHTGDL
                     IRGGTVAEYLENFLPLLEITDPVPVFCVPGNHERGARRDFAAYKAIFGGDRFSFDYGP
                     CRFVGFNDSEGIRVSGSDLAFLDEELGKPGAQYKFVFFHIPPAYFEEAAIGDGKARGF
                     TWKADAFRALLARHKVTEVFMGHIHGYTSTVLDGVRYTLSAGAGAPLSERLPEESRIY
                     HLLVVRVSPEGLSQELVHLVPGSLDWARQTIY"
     gene            5028..6167
                     /locus_tag="PKW60_03185"
     CDS             5028..6167
                     /locus_tag="PKW60_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPJ98465.1"
                     /translation="MHKRMLFALLLSLLIFPASIFAAEPGAVPKNVIVMIGDGMSIGQ
                     VTAARVVKGSLELERCSVGGFSATHSADKMVTDSAAGGTALATGLRTNNGMVAMLPDG
                     TPIKTLLEHAKDVGKSAGIVVTCSVTHATPATFVAHVDSRSKQTEVAEQIAAGNVDVV
                     LGGGQQFFTPKSASGSKRKDNQDLIAVLRERMPVALTVEELRGLGDVDSFAGLLAPDD
                     LGYAADRVITLAEMVRLSLQALSKNPNGFALMVEGSLIDWADHANDEPKMLAEMTSFD
                     DAIGAALDFAETDGNTLVVVTADHDTGGYTLLDGSLADKKVTKSAWVSGGHTAIMVPV
                     FAYGPGADALGGIHNNSAIGQKLIEYVEQSSAKAAKSGALVGAAP"
     gene            6466..7878
                     /locus_tag="PKW60_03190"
     CDS             6466..7878
                     /locus_tag="PKW60_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPJ98466.1"
                     /translation="MGACQNKSGPRRPSGRAAARKARFFRFEPAALILLGTVLLCGCS
                     VIIPEPGDKYVDGRLQITYWEKWPGFEGQAIQRVVDRFNERQDRIYVNLVTMSQIDRK
                     ALVAIAGGDPPDLVGLWSAGLSQFAEKRALMPLEPFMEKAGMSSDAFLEVFLEQNTYR
                     GTLYGLPTTPATTALHYNKRLFREAGLDPERPPRTLAELDEMAAKLTRYNENGHLVQV
                     GFTPSEPGWWPWAWGYWFGGTLWDGERVTFDTPENLAAWEWIQSYPEKYGAEELRRFQ
                     SGNTGQFASPQNAFFAERVAMIVQGVWMASFIDTFAPDLEWGAAPFPAAVPGLENVAM
                     VECDSICIPVGARHPDEAFEFMQYLCSQEGLELLNMGHKKFTPLKEVSPAFLAGHPNP
                     YIELFIELAKSPNAFYAPVMSTWYEYNDEITPAFDRVMFGESSARAAVTLVQERVTGI
                     WERARRGIERRERAEKEAAP"
     gene            7875..8771
                     /locus_tag="PKW60_03195"
     CDS             7875..8771
                     /locus_tag="PKW60_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPJ98467.1"
                     /translation="MTSREWRDFRNGLLFISPWIAGLAAFTAYPLGASLYYSFCDFSV
                     LKPPVFVGGANYQELFLDPVFWKSLFNTLYFAVFFLPLATVLAIALALLLNTGVKGMT
                     VYRTIFFLPSLTPLVALGILWMWMLNGEYGIVNYVLNLVLQPLGLHAPIWLQSTAWSK
                     PAIILMSLWGVGYAVVIYLAGLQDIPKPLYEAAELDGARWFAKLRHITLPLLSPAIFF
                     NVVMGLIGALQVFALPYVLTDGAGGPARSTLFYTMYLYNSAFRYLRMGYACAMAWLLF
                     LIILGLTLLVYRLSRKRVHYGR"
     gene            8806..9663
                     /locus_tag="PKW60_03200"
     CDS             8806..9663
                     /locus_tag="PKW60_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPJ98468.1"
                     /translation="MNRGASRANRLSLPHAFVLHALLIAGSALFLFPLVWMLSTALKP
                     LDQTMLMPPRWIPGPVQWGNFREAVRYIPFFTYARNTLTIALLGTLGTLVSSALVAYG
                     FSRIEWKGRDTVFIVLLATMMVPFPVTMVPLYAVFRSLHWIGTFRPLWVPAWFGASAF
                     NVFLLRQFFLTIPRDFDDAARIDGCTEFGIFRRVVLPLSRPALAVVALFHFMYCWNDF
                     LGPLIYLIDQDSFTLALGLQFFQSRQGGTPWNLLMAASTLVVAPIIVLFFFTQRTFIQ
                     GITMGGLKG"
     gene            complement(9713..>9941)
                     /locus_tag="PKW60_03205"
     CDS             complement(9713..>9941)
                     /locus_tag="PKW60_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPJ98469.1"
                     /translation="ANARLLDGKKATSYPGFVDKMDLPTTTYTGSAVETDGKVITSRG
                     PGTAMDFALAIIEALDGPAKRKAVEDGLMRN"
BASE COUNT         1815 a         2973 c         3175 g         1978 t
ORIGIN      
        1 ccatttgcct ggacgtcacc cgaaaacagc ggcttcaacg acgcgacttc gaacgcgaaa
       61 tcactcacga tgcggtcttt ttcaaaccgg gccacaaaat cctcgccgcc gtaatgcagc
      121 gtagcagtct gaggtttcca tgaattgtcc acacgcgcta cacggatgtc ggccgttgcg
      181 ggatcgcccg ggcgtttgtt cacatagtcg ccaatgcgca gcgccgcctg cacgaggtcc
      241 acgtgagctt caaacgaatc ctggttcagc ggcacagcgg ccgacacggc cgcggtccct
      301 tccaggtgca gtgaacccgg gacgagatcg cgaagcgcag ccaggggcac ggcggcgcgc
      361 gtgtctgcct tggaaacaga gattgcgccc ttcgcatgta ccagcgcaac ggtggccgag
      421 agttcaggag ttccctgccg cgcgatacgg acaaagcctt cgggcgcagc tgcggcaggc
      481 agggccacct cgaaatcaaa cgccgatgcc ccgtagacgc gcgcaatgtt catggcggcg
      541 ccgcgggtgg tctcatcgtt cagaaacggc agattaagct gggcgagatc ggcctggagc
      601 accccggtga cgcgctgttt ttcgggcgtg atccgcgcat cggcggttat cggcccgaga
      661 agcgttgatg tgcatgatcc cgtcactttg atcgcgccgg gttcggcggc aaagcgcccg
      721 gaaatgccgc tcagggtctc ctccagctcg ccgagcacga gggtggcctg cgccttttca
      781 agcgccccat cgattttgag gtcgtcgggg agcgcctttc caggttcaaa cgtgcccgcg
      841 atgcgtttga aggccatgcg ccccgacagc cctttgatgc gcgcatcgtc cttcagaacc
      901 gcagcaatga cgcccgggga gagcgtcaac tcggcctgga gatcgacaaa cgcgctttcg
      961 cgctgcagat cgggccggac tgaaccatgc acggcaagcc ctgagccttc gagtttgtcg
     1021 acgcgaatca ccccctcgtc gagatgcacc tgcccgtgaa tatcgccgaa cggcttggag
     1081 ccgtcggcca gggtgggttg cacaccggca aactggatct ggcctttcac cagacgcagc
     1141 gcgccttcgg catcgacgcc caccagcagg ttggtcgcgg tgatggacgc gtccttctgc
     1201 ggcgtaagct ggaggtgctc agaattgacc atggcataca cggccgcgag cgcatcgccc
     1261 ctaacctgca cctcctggag ctcgcaggcg atggcgccgt ccgcggtgcg cgtggcatcg
     1321 gccagcgcgg tcaggctggg tgcggtcagg gagagactgt tcagcgtgat ctcggaaccg
     1381 cgaatccaga gcatcccggt gaaggggccg gccgcggcgc gcaccagctc cggcgcgccc
     1441 ctggcggcga ccgcctcgcc cgcgagcttc atgccgatga gctcgccggt gacttcatca
     1501 aacgtcgcac ggacctcgaa tacgagcgga ggcatggcct cccgttccag gacgtgccgc
     1561 ggatcgatcc ctttgacttc aacccatcct gacggaatca ccaggggttc tgcgtctttc
     1621 tgaaccgtaa agagcagttc tccctcgcca cgcgaaggct ctcccagaaa cggaagcgta
     1681 ccactgaacg tgaccgggat ggcgtctgac agtacgtcgc gtgcctcgaa gtcgccttca
     1741 aacacgggct gctcgaatcc ggagacggat acgcgcgccg aacgggcccg aatccggtcg
     1801 acggcggctt tccaggaaac cggcgacggt ttggctttca tccgttcctg aatgtagtcg
     1861 atccggtcgc gaataaccgc gatttcgttt ggaagagacg ccaccagccc gtcaaaactg
     1921 acatcggtca ccgtgatctg ccgcgccgtc agcccgttcc aggtcgcagt cgcccgtgcc
     1981 cgggcgcatt gtgcctgaag ctccccctcg ccaatagaga cgttgtacgc ggccacggac
     2041 ggctgcggaa agagtgtcag ctccaggcga tcgatggata cgggcatgcc ggtgtgatct
     2101 tcaagaattt gcaccacgta gctgcggtag cggtcaatat ggatgaaacg ctccacggcc
     2161 tggatggcca cgaacagcag cacggccaca atcgcgagtg tggtcaacca tttatgacgg
     2221 cggggccagg atttccgggg aggtgtagtg atttcgttca ttgcggtctc accaatcggg
     2281 cgcgcctata acggtccggc cgttcccaaa cgcgagcctc tgggggtttt gctgggatct
     2341 gcgcccggca tttcaagaaa gtcggcctgc ctgtagtgta ccacctattg cagaggccaa
     2401 caacgccatg cggagccggc atcgccaacc ggtcgaggcc tcagcggctg gatggcatcc
     2461 gcgcctcctc atcctcaccg agccgcgccg gcggctgctt cgtgtactga acagtctgtt
     2521 gcggcacgtt attctctcga aaggaagcgg cgggaaggtc cttgtgttgt acgctgcggc
     2581 tgggcggctg cgctggaatg aaatctggtt caggcgccgc tgcggaggag ggtgagggcc
     2641 cacgagacgg ggttgatgcc gaagatcaac tggatgccgg caatcaacac gagcacgcag
     2701 ataatgcggt tgaacacctt ctcggacacg cgatgatgca tccatttgcc gagccaggaa
     2761 ccggcgggaa tgaggggaac cagaacggct ccgagcagaa gtacgtcttt ttcgatcaat
     2821 ccggcggcga tatagaaagg taatttggac aggttaatga gggtgaaggt ccaggcgacg
     2881 gtgccgacaa agagggtctt cccgaggtcc tgggcgaaga tatagaggct cgcgatgggc
     2941 cccgcggcat gagcgatggt ggaagcgaag ccggcggcca gtcccgcggc cacgcccacc
     3001 ccggcgccgg gccggaagcg gcccacccag gacatggcgg cctcttttga gatgatgtag
     3061 aaggcgaaca cgatcgcgat gacgcccaca aaacgtttga tccagcttcc gtagcgttcg
     3121 acgccttccc ggccgatcca ccaccagacc gccgcgcccg ccaggatccc gaggagtgac
     3181 gggaggtaaa ggcgcaggac ggtcttccag tccctgttgt ggcggtggtg gtaaatggcg
     3241 ttcatgtcgc agaggatcaa caggggaagc atgacggccg cggcgcggtt cacgggcatc
     3301 acaaagaaca tcagcggcat cgaaaggatc cccgcgccgc ccgcaaaccc cgacttgctg
     3361 atgccttgaa tgatgatggc cgcgctcaca aacagccaga atacggccgt cggcacgcct
     3421 tcgttgatgt aggaagaaaa catgcgcggg attataccgg ccagcgcccc gtgattcgag
     3481 cgggaaatcc gcgaaagcgc gccagtaaac accgcgatgc ctgcgaaagg gaattgtcaa
     3541 caagcccaca tttggataca atgcgtacag tctttgagat gggtgagcag tatggattta
     3601 gggcatctaa cagagaaaag acacgtgatc ttctacttaa gatgcgggat acaaacgact
     3661 tctaaatacg ccacctgagt gcgactgttt gacctccagg cagggttttt ccaattgggc
     3721 gcgtcacacc tgagaattgg cctctgagca ccagtcggcg cgcgtttccg cagggtattg
     3781 atcttctccg gttcgggatc tggccgggtt tacgcgggta gcatggaaat ctccggctcg
     3841 cctttttcaa agctctaccg cgtgttacca tattcggctt ggaacaaacc cggcacattg
     3901 ggtgttgcct tatggatgtt gggccggggg tacggtctcc ggcagatccc gtgccgtcag
     3961 caaaggatgg tgtaaccgtg ccgcgaccat tggcgaaaca taagctggtt ctagccgggc
     4021 tggcgccgct ggcgctggta tgcattctgg cggtaagctg gtacgtgacg gcctccccgg
     4081 gtgatctgga gaagctcgca ccgtacgccg agaaatacac gctgacgaaa ttgctcgaca
     4141 cgctgcaggc aagtgggcct gctcccgaga ccttctcgtt tgtggtgctg ggcgatacgc
     4201 gcaacaacgt tttcgtggca tccaagatat ttgaacaggc agcgcaggaa gcccccacgc
     4261 tcgcctttca tacgggtgac ctgatccgcg gcgggacggt cgcggagtat ctggagaatt
     4321 ttcttccgct gcttgaaatc acggatccgg tgcccgtgtt ctgtgtgccg ggcaaccacg
     4381 agcgcggcgc gcggcgcgat ttcgcggctt acaaagcgat cttcgggggc gaccggtttt
     4441 cttttgatta cgggccctgc cgttttgtcg ggttcaatga cagcgagggc attcgcgtct
     4501 ccgggtccga tctggcgttt ctggatgagg aactcgggaa acccggggct caatacaaat
     4561 ttgtcttttt tcacatcccg ccggcctatt tcgaagaggc ggccatcggc gatggcaagg
     4621 cgcgcgggtt tacctggaag gccgacgcct ttcgtgcgtt gctggcacgg cataaagtca
     4681 ccgaggtgtt catgggccat atccatggat acacgtcgac ggtgctcgat ggcgtgcgct
     4741 acaccctttc agcaggcgcg ggcgctccat tgagcgaacg gctcccggaa gagtcgcgaa
     4801 tttatcattt gctggtggtg cgagtctctc cggaaggtct ttcgcaggag ctggtgcatc
     4861 tcgtgccggg ctcgctggac tgggcgcgcc agacaatcta ttgatccgga ggcgatgtgc
     4921 agcgcgccga aggagagagt aacccggcat gggccggttg ctgaaaatag agtgaaactg
     4981 gagacggggc gctcggcgcc cgagataagg ataggagacg ttgtacaatg cataaacgca
     5041 tgcttttcgc tctgttgttg tcccttctga tttttcccgc gagcattttc gccgctgaac
     5101 cgggcgccgt tccgaaaaac gtgattgtca tgatcggcga cggcatgagt atcggccagg
     5161 tcacggcggc gcgggtcgtg aaaggcagcc tcgagctgga acgctgttcg gtgggaggct
     5221 tttctgccac gcattccgcc gacaaaatgg tgaccgattc cgctgccggc ggtacggccc
     5281 tggccacggg actgcgcacc aacaatggca tggtcgccat gctgcccgac ggcacgccga
     5341 tcaaaacgct gctcgaacat gccaaagacg tgggtaaatc cgcgggcatc gtggtaacgt
     5401 gctccgtcac ccatgccacg cccgcgacgt ttgtcgcgca tgtggacagc cgcagcaagc
     5461 agaccgaggt ggcggaacag atcgcggcgg ggaatgtcga cgtggtgctt ggcggaggcc
     5521 agcagttttt cacgccgaaa tccgcatccg gcagcaagcg gaaggacaac caggatctta
     5581 tcgcggtgct ccgggagcgc atgcctgtag cgctgacggt agaggaactg agaggactgg
     5641 gtgatgtgga ctcgttcgcc gggcttctcg cgcccgatga tctcggttat gccgcagacc
     5701 gcgtgatcac ccttgcggaa atggtccggc tgtcccttca ggctttatcg aagaatccca
     5761 acggttttgc cttgatggtc gagggttcgc ttattgactg ggcggaccat gccaacgacg
     5821 aaccgaaaat gctggccgaa atgaccagtt ttgatgacgc catcggcgcc gcgctcgact
     5881 ttgccgaaac cgacggcaac acgctggtgg tcgtaaccgc ggatcacgac acgggtgggt
     5941 ataccctgct cgacggttct cttgccgaca agaaggtcac gaaatcggcg tgggtaagtg
     6001 gcggccacac cgcgatcatg gttcctgtgt tcgcgtatgg ccctggagcc gacgccctgg
     6061 gcggcatcca caacaacagc gctatcggcc agaagctgat cgaatacgtg gagcaatcct
     6121 ccgccaaagc ggccaagagc ggcgcgcttg ttggcgcagc accctgaccc cgcgattacc
     6181 tgcacacata gacggttttc ggccccggag cccgcccttg cctgaccggc ggcggcccgg
     6241 ggccgccttt ttacatgcca gcccgattca gacgagcccg cggggtgcac gcagaaccgc
     6301 gcagcgtgct atgcggcgat cgtctggccc ggcgtttcat tcgcgcggcc gaaccggtct
     6361 ctcgctctgt cctgacaagg ccattcccct gggcgccgga aaacgggtat gctgaaggcg
     6421 tctggccttg cggccagcca ttcgtacgca ggctggaggg gagttgtggg agcgtgccaa
     6481 aacaaatccg ggccacggcg gccatccggc cgcgccgctg cccgaaaagc acggtttttc
     6541 cgattcgagc ctgccgccct gattctcctg ggcacggtct tgttgtgcgg atgctccgtc
     6601 attatccctg agcccgggga taagtatgtc gacggccgcc tgcaaatcac gtactgggag
     6661 aaatggcccg ggtttgaggg ccaggccatc caacgggtgg tggaccggtt taacgagcgg
     6721 caggaccgca tctacgtcaa cctggtcacg atgagccaga tcgaccgaaa ggcgctggtg
     6781 gcgatcgccg gaggcgatcc gccggatctg gtgggcctgt ggagcgccgg cctgtcgcag
     6841 tttgccgaaa aacgggccct catgcctctg gaacctttca tggagaaggc ggggatgtcc
     6901 agcgatgcgt tcctggaggt gttcctggag cagaacacct accggggcac actctatgga
     6961 ctgccgacaa cgcccgccac gacggcactg cactacaaca agcggctgtt ccgcgaggcg
     7021 gggctggacc cggagcgccc gccccggaca ctggctgaac ttgatgaaat ggccgcgaaa
     7081 ctgacccggt acaacgaaaa cggccacctg gtgcaggtgg gattcacgcc gtcggaaccg
     7141 ggatggtggc cctgggcgtg ggggtactgg tttggaggga cgctgtggga cggcgagcgc
     7201 gtgacgtttg acacccccga gaacctggcc gcctgggagt ggatccagag ctatcccgag
     7261 aaatacggcg cggaggagct gcgccggttt cagagcggga atacggggca gtttgcatcg
     7321 ccgcagaatg cgttttttgc cgagcgggtg gccatgatcg tgcagggggt gtggatggcg
     7381 agctttatcg acaccttcgc gcccgatctg gaatggggcg ccgcgccctt tcccgcggcg
     7441 gtgccggggc tggagaacgt ggccatggtt gaatgcgact cgatctgcat tccggtgggc
     7501 gcccgccatc cggacgaagc cttcgaattc atgcagtacc tgtgttcgca ggaagggctg
     7561 gaattgctca acatgggcca caagaagttt acgccgctca aagaggtcag tcccgccttt
     7621 ctggccgggc atcccaatcc gtatatcgag ctgttcatcg aactggcgaa gagtcccaat
     7681 gcgttctatg cgccggtcat gagtacgtgg tacgaataca atgatgagat cacaccggcc
     7741 tttgatcgcg tcatgtttgg cgagagctcg gcgcgggcgg ccgtgacgct ggtgcaggag
     7801 cgggtcaccg gcatatggga gcgcgcgcgc aggggcatcg aacgccgcga acgcgcggaa
     7861 aaggaggcgg cgccatgacg agccgcgaat ggcgagactt ccgcaacggg ttgctgttta
     7921 tttcgccgtg gatcgccggg ctggccgcgt tcaccgccta tccactcggt gcgtcgctct
     7981 actacagctt ctgcgatttc tccgtgctga aaccgcccgt gtttgtgggc ggcgcgaact
     8041 accaggagct gtttctggac ccggtcttct ggaaatcgct tttcaacacg ctgtatttcg
     8101 cggtgttctt tctgcccctg gcgacggttc tggcgatcgc gctggccttg ctgctcaaca
     8161 cgggcgtcaa aggcatgacc gtgtaccgca cgatcttctt tctgccgtca cttacgccgc
     8221 tggttgcgct gggcattctg tggatgtgga tgctcaacgg cgagtacggc atcgtgaact
     8281 acgtgctcaa cctggtgttg cagccgctgg ggctacacgc gccgatctgg ctgcagtcga
     8341 cggcctggtc gaagcccgcg atcatcctca tgagcctctg gggggtgggg tatgcggtcg
     8401 tgatctatct cgcgggactg caggacatcc cgaagccgtt gtacgaggcg gcggaactgg
     8461 acggcgcccg gtggtttgcg aaactgcgcc acatcacgct gccgctgtta tcgccggcga
     8521 tcttcttcaa cgtggtcatg ggcctgatcg gggcgttgca ggtgtttgcg cttccgtatg
     8581 tgctgaccga cggcgcgggc gggccggcgc gctcgaccct tttctatacc atgtacctct
     8641 acaattccgc gtttcgatac cttcgtatgg gctatgcgtg tgcgatggcc tggctcctgt
     8701 ttcttatcat cctcggcctg acgctgcttg tgtatcgcct gtcgcgcaaa cgggtgcatt
     8761 atggtcgata ggcctgccca cacactgacg gaaagcccgc ggcgcgtgaa tcgcggcgct
     8821 tcccgggcca accggctgag cctgccgcac gccttcgtgc ttcacgccct gttgatcgcg
     8881 ggaagcgccc tgttcctgtt cccgctggta tggatgctgt cgacggccct taaacctctg
     8941 gaccagacga tgctcatgcc gccgcgctgg attcccggcc cggtgcaatg ggggaatttc
     9001 cgcgaggcgg tccgttatat cccctttttc acctatgcgc gcaacacgct gaccatcgcc
     9061 ctgctcggca cgctcggcac cctggtcagc agcgcgctcg tcgcgtacgg gttttcgcgg
     9121 atcgagtgga aaggccgcga caccgtgttt atcgtgctgc tcgcgaccat gatggtgccg
     9181 tttccggtga cgatggtgcc gttgtatgcg gtgttccgtt ctttgcactg gattggcacg
     9241 ttccggccgc tgtgggtgcc cgcctggttc ggagcgagcg cgttcaacgt cttcctgttg
     9301 cgccagttct ttctgacgat cccgcgggac ttcgatgatg cggcgcgtat agacggctgc
     9361 acggaattcg gcatcttccg gcgggtggtg ctgcccctgt cgcgtccggc gctggccgtg
     9421 gtggccctgt ttcatttcat gtactgctgg aacgatttcc tggggccgct gatctacctt
     9481 atcgaccagg acagcttcac gctggctctc gggctccagt ttttccagag ccgtcagggc
     9541 ggcacgccgt ggaacctgct catggcggcg tcaacgcttg tcgtagcgcc gatcatcgtg
     9601 ctcttctttt tcacgcagcg cacgttcatc cagggcatta ccatgggcgg attgaagggc
     9661 tagcccgcgt cccccgggcc gtgtcacgcc gccgtggatg gctcgcgagc gctcagttcc
     9721 gcatcagccc gtcctcgacc gcctttcgct ttgcgggtcc gtcaagcgcc tcgatgatgg
     9781 ccagggcgaa gtccatggcc gttcccggtc cgcgcgaggt gatgactttg ccgtctgtct
     9841 ccacggcgct gcccgtatag gtcgtcgtgg gcaggtccat tttgtcgacg aatccggggt
     9901 agctggtcgc ttttttccca tccagcagcc gggcattggc g
//
LOCUS       DAOPBQ010000058         9873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6585_length_9873_cov_4.542066, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000058 DAOPBQ010000000
VERSION     DAOPBQ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6585_length_9873_cov_4.542066"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PKW60_03210"
     CDS             complement(<1..869)
                     /locus_tag="PKW60_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPJ98470.1"
                     /translation="MRALALHCPFKTLHRYVLREIAIPSVLAFALISFLAVGNELRER
                     VEQLPIEHISPLDIVRLTAFFLPSVVPFIIPITYMMGLLLAFGHLNQQNEIIAMKAAG
                     IPLKRVVIPVLLGGALLSVAAFVIQDRVQPFTFRSAMLMITRELPSRLTLDVLPAGVM
                     HEIQSEQGVWRIYIERKDAETGTLYNIELLMPRKEDEAWTFFAREAQIITSDAQTEII
                     LRDGYSIYPQDDGEQLLVSTFPEFRVPWPHSLPGKIPQERRTLNLAGCLAAERELNRL
                     YEARRTDKLKGDLR"
     gene            1127..2467
                     /locus_tag="PKW60_03215"
     CDS             1127..2467
                     /locus_tag="PKW60_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98471.1"
                     /translation="MGTAVSTNVFPQLSQPLTTPAQSEFRVFGPQPGEGAALPNLLTS
                     AAQGKDSAAVLGFNGGAADTFDAATYGARVQRVSLAAEFQRVRERIAAVQEGGGNTVA
                     AAGVEQLEFRFVAESRQEELAIFQRRTAEVAAGLKGRQSESFVQASRQMAARFSLSVK
                     LSGAALNGFAGAAEGLKNGGPDSLDQFIGFAQDALKQLDEKVSELFSLLHDLVQSGGE
                     LRERIDSFIAELRSLGLLGGGEAAAGPGNTLSAAAQTRSFSIQLTFEFEYQEVLQVQQ
                     GAVQQSDPIVFDLDGDGIELTSYTQGARFDITGTGKKVTTGFVTGGDAFLAIDRNGNG
                     RIDDGTELFGDQNGAANGFEELRRLDTNGDGRINQLDANFNKLLLWRDNGNGITEPGE
                     LISLADAGIVEISLGYRNVNQSAAGGNRITQVATFVRADGSRGTAADALLNYLA"
     gene            2622..3596
                     /locus_tag="PKW60_03220"
     CDS             2622..3596
                     /locus_tag="PKW60_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015846856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HPJ98472.1"
                     /translation="MEYRKLGKWGAKVSSLSLGTYLTVGFTSDAETSKLLVKTALDAG
                     INYFDTADAYNRGGAEKALGELVAGVKRSDLFILTKVWAPMGDGPNDRGLSAKHIKES
                     CDGSLKRLGMDYVDCYMCHRPDADTPIEETILAMEDLIRAGKVIYWGVSEWPAPLLVR
                     ANERAAQLGARPIAVSQPRYNLLYRYPETLLFPTTAEEGIGNVIFSPLAHGMLTGKYK
                     PGEEAPEGSRASDDRQNLVIKAMYWTEEYKKKGQELAAIAKDFGVSAAELALAWCLRN
                     PNISSVILGASRVEQLQQNLKALDLKLTDDVVTRLETLYPQPEAIPQI"
     gene            3773..5197
                     /locus_tag="PKW60_03225"
     CDS             3773..5197
                     /locus_tag="PKW60_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPJ98473.1"
                     /translation="MGGLFGVVSKNDCVHDLFYGTDYHSHLGTSRGGLAVLNGEAYTR
                     RIHDISTAQFRSKFEGDLQYMQGRSGIGVISDSQSQPLLIGSHLGPYAIVTVGAIRNA
                     DALARQAYKKRTVHFSEMSGGGINPTELFATLINQEATFEDGILAAQQTIEGSCTLLL
                     LTHQGIYAARDRYGRTPLAIGKKDGAYAVTFETAALPNLGYTLDRFLGPGEIVRITAD
                     GLEQKAPPGDELRVCSFLWVYYGFPASSYEGINVEAVRNRCGAALAAGDEQDIDFVAG
                     IPDSGTGHAIGYANAAKVPYARPYVKYTPTWPRSFMPQAQDMRELIARMKLIPIVDFI
                     QGKRLLFCEDSIVRGTQLRDTIQQFYDYGAQEVHMRSACPPLVYGCEFLNFSRSKSAL
                     DLAARRAIHELEGDGDEELPKYTDESTPQYAAMVDRIRQRLSLTTLKYQRLDDLVKAI
                     GLPKTKLCTYCWDGCQGCAPKGSV"
     gene            complement(5321..6304)
                     /locus_tag="PKW60_03230"
     CDS             complement(5321..6304)
                     /locus_tag="PKW60_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPJ98474.1"
                     /translation="MRRRSDRGFTLIELLVVIAIIGILAAILLPALARAREAARRASC
                     ANNLKQMGIIYKMYANESKGEKWPPLQGIAEYYTDGVPGGLPAGCKGQDEPELAPRQI
                     AIYPEYLTDYNVIRCPSDPDFSDAMEDHLAIIRDVPPAVCNYIYKGVADNPSDSYFYL
                     GYVADQCNGDDPLMPDPFGMGLQVPVQLMLVLGVIGPLSLGGNGAFGTVEAANPVAVR
                     SSLDNDISIPSPFGNGGGGTIYRLREGIERFMVTDINNPAGSAQAQSEIVVMFDCISL
                     TPGGDIPFNHVPGGSNVLFMDGHVEFFRFEKLGRFPVNAPFASIVELVGYL"
     gene            complement(6560..8032)
                     /locus_tag="PKW60_03235"
     CDS             complement(6560..8032)
                     /locus_tag="PKW60_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98475.1"
                     /translation="MPIPLNERRARNQLRKRGATGLQIRQWLALHQRTAFARYFIRDS
                     KDRPWVARRYQEASLNSYALRKVHCDGRDVGKTAEIELIAAWASVLCPNTEMLVATQC
                     ENHLFPLMHRLLRRFQSTPHFAPGLLEVKRSPSWFFRFSNGFVLWGRIAGPHGMNFQG
                     LHVDWQIVDEAQEMTEPAWAELFQALNGGGRRWVYGVPNGLRNTFYRMTQDGAAEQYN
                     WPSPLNPEYTPEKDAELARLYGGRGSPGYVHRVLGQHGTPAHAVFDFEAYLACIDEGL
                     DFHDVHLTADDAFSLPAALPPGDYYLGCDLGYARDPSEFVVYRAAEPHFINVLRVHLE
                     GVHYARQQEIITELDRACDFRRIGIDCGNSGRAVAHNLMAQGTQWCEKVAAFEFGASI
                     DLEPLPDGSPHRRRVKQFMTELLERRMQERSIVFPKLPDRESQYAGHIYTISATGDVI
                     YEKGDDHIIDADRCAVLARYLDTQCGESEAPLLGVRLEGF"
     gene            complement(8035..8535)
                     /locus_tag="PKW60_03240"
     CDS             complement(8035..8535)
                     /locus_tag="PKW60_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98476.1"
                     /translation="MRFLSDLLRNHPGGGWALLERDAEVETLAGCLETVLKPFLEGQG
                     AGLLPLVALRAEELVAAWLIARRMEDILRAEGVCHMHVVEGELRFKEVHPMIEKSMKA
                     RERVRKALQELEDACKATAVSGGSGALGEEVSPLLRLGEGVLEDALNFEKRKQKRSRS
                     GRAANA"
     gene            complement(8905..8982)
                     /locus_tag="PKW60_03245"
     tRNA            complement(8905..8982)
                     /locus_tag="PKW60_03245"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8944..8946),aa:Glu,seq:ttc)
     gene            9397..9576
                     /locus_tag="PKW60_03250"
     CDS             9397..9576
                     /locus_tag="PKW60_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98477.1"
                     /translation="MNEEGETLSRWAFWGHLAAAVWILGTTVLYFVRLSAVFFRDNHD
                     TLNGLLERLGLGFLG"
BASE COUNT         1966 a         2922 c         3111 g         1874 t
ORIGIN      
        1 cggaggtcgc ctttgagttt gtccgtgcgc cgcgcctcat acaacctgtt gagttcgcgc
       61 tcggcggcca ggcacccggc caggttcagg gtgcggcgct cctgcgggat cttcccgggc
      121 aggctatgcg gccagggcac gcggaattcg ggaaaagtgc tgaccagcag ctgttctcca
      181 tcgtcctggg ggtagatgga atagccgtcg cgcagaataa tctcggtctg agcgtcactg
      241 gtaatgattt gggcctctcg cgcgaaaaac gtccacgctt cgtcctcttt tcgcggcatg
      301 aggagctcga tattgtacag tgtgccggtc tcggcgtctt ttctctcgat gtagatgcgc
      361 cagacgccct gttcgctctg gatctcgtgc atcacgcccg ccggcaacac gtcgagggtg
      421 aggcggctgg gcagctcgcg cgtgatcatc agcatggcgc tccggaaggt aaagggctgc
      481 acccggtctt ggatcacaaa cgccgctacg ctgaggagtg cgccgccaag cagcacgggg
      541 atcaccaccc gtttaagcgg gatgccggcg gctttcattg cgataatttc gttttgctga
      601 ttcaaatgcc cgaaagcgag caaaagcccc atcatgtagg taatgggaat gatgaagggc
      661 accacgctcg gcaaaaaaaa ggccgtcagc cggacaatgt cgagcggcga gatgtgttcg
      721 atgggcaact gctcgacacg ctcccgcaac tcgttgccga ccgccagaaa gctgatgagc
      781 gcaaacgcca gcaccgacgg tatcgcaatc tcgcgcagga cataccggtg aagagtctta
      841 aatgggcagt gtagtgccag ggcacgcatg gcacgagtat agcgtaagct gtggatacct
      901 ctccacttgc catgaagccg gccgtgaagc tgcgcgaggg cgcaggcacg tggttccgtt
      961 cagatgcaca cgaacaatgc gccgttctta tccttagaaa acgggctgcg ggcgggctgc
     1021 aggatgttcg cgctaaagca cggggccctt tccgccgata cactggattg gcaacaacca
     1081 tgggaggccg gttccgcatc gctggaacgg gccgggagga actatcgtgg gtaccgctgt
     1141 ctctacgaat gtctttccgc agctcagcca gcccctcacc acgccggcgc agtcagaatt
     1201 ccgtgttttc ggcccgcagc cgggcgaagg cgccgcgctg cccaacctct tgacctcggc
     1261 agcccagggc aaagactccg ccgctgtact gggtttcaac ggcggcgctg cggacacgtt
     1321 tgacgccgcc acctacggcg cgcgggttca gcgggtaagc cttgccgcgg aattccagcg
     1381 cgtgcgggaa cgcatcgccg cggtacagga aggcggcggc aatacggtgg cggcggcagg
     1441 tgtagaacag ctcgaattcc gtttcgttgc tgaatcgcgc caggaggaac tggcgatctt
     1501 tcagcgccgg actgctgaag tggccgcggg gcttaaaggc aggcaaagcg aatccttcgt
     1561 gcaggccagc cggcagatgg ccgcccggtt ttcgctctca gtaaagcttt ccggggctgc
     1621 cctcaacggg tttgcgggcg ccgcggaagg tctcaaaaac ggaggcccgg actcgctgga
     1681 ccagttcatc gggtttgcac aggacgcgct gaagcagctg gacgaaaaag tcagcgagct
     1741 cttttctctg ctgcacgacc tcgtgcagag cggaggcgag ttgcgggagc gaattgactc
     1801 gttcatcgcg gaactgcgct ccctggggct cctcggaggc ggcgaggccg ccgctggacc
     1861 ggggaatacg ctttccgccg cggcgcagac ccgcagtttc tcaatccaat tgacgttcga
     1921 gtttgaatat caggaagtgc tccaggttca gcagggggcc gtacagcaat cagacccgat
     1981 tgtatttgat ctggacggcg acggcattga gctgacgagc tacactcagg gggcacgttt
     2041 cgatataacg ggaacgggaa aaaaggtgac cacagggttt gtgacgggcg gcgatgcgtt
     2101 tctggcgatc gatcgcaacg ggaacggccg catcgacgat ggcaccgaat tgttcggcga
     2161 tcagaacggc gccgcgaacg ggtttgagga attgcgccgg ctggatacca atggcgatgg
     2221 gcgcatcaac cagttggacg cgaatttcaa taagctcctc ctgtggcggg acaacggcaa
     2281 tggcataacc gagccgggcg agctgatcag tctcgcggac gcggggattg tggagatctc
     2341 gctgggctac cgcaacgtga accagtctgc agcgggcggc aaccgcatca cacaggtcgc
     2401 gacgttcgtg cgcgcggacg gttcccgcgg gaccgcggct gacgcgctcc tcaattacct
     2461 cgcgtagtct cggaggcata tttccggcca gctacagtac tttcctgatg gccccgtgcc
     2521 tggtgcgtcc agtcgcgggg ccatctcttg agttcgtcag gcaaccgggt taggatttcc
     2581 aaagaaacaa atgcgtgacg gaccgcagga ggggaagggc tatggaatac cgcaaacttg
     2641 gaaaatgggg cgcaaaagtc agttccctga gcctggggac gtacctgaca gtagggttca
     2701 cctcagacgc cgagacatcg aagttgcttg tcaagactgc gctggatgcc gggatcaact
     2761 atttcgacac ggccgacgcg tacaaccggg gcggggcgga aaaggccctg ggcgagctcg
     2821 tggctggcgt gaaacgctcc gatctcttta tcctgaccaa ggtgtgggcg cccatgggtg
     2881 acgggccaaa cgaccggggc ctgtcggcaa aacatatcaa agaatcgtgc gacggcagcc
     2941 ttaaacgtct gggcatggac tatgttgact gctacatgtg ccaccgtccc gacgccgata
     3001 cgccgatcga agagaccatt ctggccatgg aagacctgat ccgggcgggc aaggtgattt
     3061 actggggcgt cagcgaatgg ccggcgccgc tgctcgtccg ggcgaatgag cgggcggccc
     3121 agctgggcgc gcgcccgatc gccgtgagcc agccccggta caatctgctc taccggtacc
     3181 ccgaaacgct gctgtttccc actacggctg aggaaggcat cggtaacgtg attttctcgc
     3241 cgcttgccca cggcatgctg accggaaaat acaagccggg cgaagaggcc cccgaaggca
     3301 gccgcgcctc cgacgaccgg cagaacctcg tgatcaaagc catgtactgg accgaagaat
     3361 acaagaaaaa gggccaggaa ctggcggcca tcgcgaaaga cttcggcgtc agcgccgcgg
     3421 agctggcctt ggcctggtgt cttcggaacc ccaacatcag cagcgtgatc ctgggagcgt
     3481 ctcgggtcga acagctgcag cagaatctca aggcgctcga tctcaaactg accgatgacg
     3541 tggtgacccg tctcgagacg ctgtatccgc agcccgaggc cattccgcag atttgaaccg
     3601 cgcgcgcctg aaccgcgcgc agagtacgat gcgttgtgcc ggcgcctgcg tgggcatgcg
     3661 cggaggcgga tccgcttgcc cggcgcccgc gcaatgcggt acattccacg gcagagggat
     3721 ggctcctcct gccccgctat tcttgtccgg ccattgttaa aggagaaccg ccgtgggtgg
     3781 actgtttggc gtcgtgtcga aaaatgattg cgtacacgat ctgttctacg gaaccgacta
     3841 tcattcgcat ctgggaacgt cgcgtggggg gcttgccgtg ctgaatggcg aagcctatac
     3901 ccgccgcatt cacgatattt cgacggcgca gtttcgctcg aaattcgagg gcgatctcca
     3961 gtacatgcag gggcggagcg gcatcggcgt catcagtgat tctcagtcgc agccgttgct
     4021 tatcggctcg cacctgggcc cgtatgccat tgtgacggta ggcgccatcc gcaatgccga
     4081 tgcgctcgcc agacaggcct acaagaaacg gacggtccat ttttcggaga tgagcggcgg
     4141 aggcatcaac ccgaccgaat tgtttgcgac gctcatcaac caggaagcca cgttcgaaga
     4201 cgggattctg gcagcccagc agaccatcga gggctcgtgt accctccttt tgttgacgca
     4261 tcagggcatt tacgccgcgc gcgaccgtta cgggcgtacc ccgctggcca tcggcaagaa
     4321 agacggggcc tatgctgtca cgttcgagac ggcagcgctc cccaatctcg ggtacacgct
     4381 ggatcgtttc cttggtccgg gcgagatcgt ccggatcacc gcggacggac tggagcagaa
     4441 agctccgccc ggcgatgagc tgcgggtttg ttcctttttg tgggtgtatt atgggtttcc
     4501 ggcctcgagc tacgaaggca tcaacgtcga ggccgtgcgc aaccgttgcg gagcggcgct
     4561 ggctgccggt gatgaacagg acatcgattt cgtggcgggg attccggatt ccggcacagg
     4621 ccacgccatt ggctatgcca atgcggccaa ggtcccttac gcgcgcccgt acgtgaaata
     4681 cacccccacc tggccgcgca gtttcatgcc gcaggcccag gacatgcgcg agctcatcgc
     4741 ccgcatgaaa ctcatcccca tcgtcgactt tatccaggga aaacggctgt tgttctgcga
     4801 ggactcgatt gtgcggggca cgcagctccg ggatacgatc cagcagttct acgattacgg
     4861 ggcccaggaa gtgcatatgc ggtcggcgtg cccgccgctg gtctacggtt gcgaatttct
     4921 gaatttctcg cgctcgaagt ccgctctgga cctggctgcc cgccgcgcca tccacgagct
     4981 cgaaggcgat ggcgacgaag agttgcccaa gtacaccgat gagtcgacgc cccaatatgc
     5041 ggctatggtc gaccggatcc ggcagcgtct ttccctgacg acgctgaaat accagagact
     5101 tgacgacctg gtgaaagcca tcggcctgcc caagacgaaa ctatgcacct attgctggga
     5161 tggctgccag ggatgcgcgc cgaagggctc cgtataactg gagcgggacc cggacggccc
     5221 gcccgctcca gtccgcactg gcgctgatgt gtggcagccg ggccatcgag cgttcgcgca
     5281 tcgcggcccg gctgcgaaat catgcacgct gttgcagcgg ctagagatac cccaccagct
     5341 cgacaatcga cgcgaaaggc gcattaaccg ggaatcgccc gagtttttca aagcggaaaa
     5401 actcgacgtg cccgtccatg aacaacacat tggagccgcc gggcacgtga ttaaacggga
     5461 tgtcgccgcc gggggtcagg ctgatgcagt caaacatgac cacgatttcc gattgggcct
     5521 gggcgcttcc ggcgggattg ttgatgtcgg tcaccatgaa ccgttcaatg ccttcgcgca
     5581 gccggtagat cgtcccgccg ccgccgttgc cgaacgggct gggaatcgaa atgtcgttat
     5641 ccagggagct gcggaccgca accggattgg cggcctcgac tgtgccgaac gcgccatttc
     5701 ctcccagaga cagggggccg atcacgccca ggaccagcat caactgcacc ggcacctgca
     5761 gccccatccc gaacggatcg ggcatcagcg gatcatcgcc attgcattgg tcggccacat
     5821 atcccaggta aaagtaactg tcggaggggt tgtcagccac gcccttgtag atgtagttgc
     5881 acacggccgg gggcacatcc ctgatgatgg ccaggtggtc ttccattgcg tcgctgaaat
     5941 cggggtcgga aggacagcgg atgacattgt aatcggtcag gtattccgga tagatcgcga
     6001 tttgacgcgg ggccagctcg ggttcgtcct ggcctttgca gcccgcgggc agccctccgg
     6061 ggacaccgtc ggtataatac tcggcgatac cctgaagcgg aggccatttt tcgcccttgc
     6121 tttcgttcgc atacattttg tagatgatgc ccatctgctt gagattattg gcgcagctgg
     6181 cgcgccgtgc ggcctcgcgc gcccgcgcca gcgccggcag aagaatggcg gccaggatgc
     6241 cgataatcgc gataactacg agcagctcaa tcagtgtaaa tccccggtct gatcttctac
     6301 gcattgctat gtctcctctt gtggaaaatg acacggttgg catgcgattc gatcgtcgta
     6361 agggtgtagc cgtgttcccc atcgcacctc ccgtctgaca actggatacg cagacaggta
     6421 acagcctgcc tgccctcgtg tgtacgaagt tccagccgcc tctgtatatc gtcgaatcag
     6481 actacccttc tacgcgcatt tcgtcaatgg tttcagcgaa cgcggaaggc ggcggcgcta
     6541 aggcgttgaa aaggcgtgat caaaagcctt cgaggcgcac gcccagcagc ggcgcttcag
     6601 actccccgca ctgcgtgtcg aggtaacggg cgagaacggc gcaccgatcg gcgtcaatga
     6661 tgtggtcgtc gcccttttcg tagatcacgt cgcccgtggc ggagatggtg tagatgtggc
     6721 cggcatactg ggattcacgg tcgggaagtt ttggaaacac gatgctgcgt tcctgcatcc
     6781 tgcgctcgag cagctcggtc atgaactgtt tgacccggcg ccggtgggga gaaccgtcgg
     6841 gaagaggctc aaggtcgatg gatgcgccga attcaaaagc agccactttt tcgcaccatt
     6901 gcgtgccctg cgccataagg ttgtgtgcaa cggcgcgccc gctgtttccg caatcgatgc
     6961 cgatccggcg gaaatcacag gcgcggtcga gttcggtgat gatctcttgc tggcgggcgt
     7021 aatggactcc ctcgagatgc acccgcagca cgttgatgaa atggggttca gcggcgcggt
     7081 acacgacaaa ctcggaaggg tcacgggcgt agcccaggtc gcagccgaga tagtagtcgc
     7141 cgggcggcag cgctgccggc agggaaaatg cgtcgtccgc ggtgaggtgc acatcgtgga
     7201 aatcgagacc ttcgtcgatg caggcgaggt aggcctcgaa atcgaacacg gcgtgggcgg
     7261 gcgtgccgtg ctggcccagc acccggtgca cataaccggg ggagccgcgg ccgccgtaca
     7321 gccgggccag ttcggcgtct ttttcaggcg tgtattcggg gttgagcggc gacggccagt
     7381 tgtactgttc cgcggcgcca tcctgtgtca tgcggtagaa ggtgttgcgc agtccgttgg
     7441 gaactccgta gacccagcgc cgcccgcctc cgttcagggc ctgaaacagc tcggcccatg
     7501 cgggctcggt catctcctgg gcttcgtcga cgatctgcca gtccacgtgc agcccctgga
     7561 aattcatgcc gtgaggcccg gcgatgcggc cccacaagac gaacccgttg gaaaaccgga
     7621 aaaaccagga aggcgagcgc ttcacctcga gcaggcccgg agcaaagtgg ggtgtagact
     7681 gaaaccggcg cagaagacgg tgcatcagcg gaaagagatg gttttcgcac tgggtcgcca
     7741 cgagcatctc ggtattcggg cacagcactg aggcccacgc cgcaatcagc tcaatctcgg
     7801 cggtcttacc cacgtcgcgg ccgtcgcaat gcactttccg gagggcgtag gagttgagcg
     7861 aggcctcttg gtatctgcgc gccacccatg gccgatcctt cgagtcgcga atgaaatacc
     7921 gcgcaaatgc cgttcgctgg tgcagagcca gccactggcg aatctgcagc ccggtcgcgc
     7981 cgcgtttccg caactggttc cgcgcgcggc gctcattcag tgggatgggc acgatcacgc
     8041 gtttgcggca cggcccgacc gggagcgctt ctgcttacgc ttctcgaagt tcagcgcatc
     8101 ctccaacact ccttcaccca aacgcagcag cggactcact tcttcgccga gagcgccgct
     8161 gcctcccgac acggccgtgg ctttgcatgc gtcctccagt tcctgcagag ctttccggac
     8221 tcgttcccgg gctttcatcg atttctcgat catggggtgg acctccttga agcgcagctc
     8281 accctccacg acatgcatgt gacagacgcc ttctgcccgg aggatgtctt ccatacgccg
     8341 cgcgatcagc caggccgcaa ccagttcttc ggcgcgtaac gccaccagag gcagcagacc
     8401 cgctccctgt ccttccagaa aaggcttcag gaccgtttcc aggcagccag caagcgtttc
     8461 cacctcggca tcgcgttcca ggagtgccca tcccccgccc gggtggtttc gcagtaaatc
     8521 ggataaaaat cgcacgaaaa gctcctgtcg agccaaaaat aggcggcaaa agccgcccgc
     8581 cagcgtcttc atatatatag cccgtccatt gccgtataaa tgaaggcaca gagcgcgatt
     8641 ttttctgaat tctccctcaa ttcggcttct cccgcacaca acggccgcgt tgttgcgccg
     8701 ggatgtccat atcatgacgc gcagcgaagg aattggcagg cggtgggttg cggcgagcgc
     8761 agtgtgttgc gggcggggtt tgcttgagcg tgcggaaacg cagccgaggc caaacgcgcg
     8821 cctccttgcg atctgacacc gatccccccg gaatgcaatg aaaaacgccg tgaaaaatcg
     8881 gggcttgtcc ctggcacaaa aaaatggcga ccccaacggg atttgaaccc gtgtcgccag
     8941 cgtgaaaggc tggtgtccta ggccagacta gacgatgggg tcgccgagta tcgagcgcaa
     9001 aaatacgcgg cagccgcttg ggcagcgccg cgtaaccgcc tcgaatatat accatatgct
     9061 ggaagctgaa ttcaaatcca tgcattttaa ggccaggtgc acagtcttta gagtttgttt
     9121 ttcatggtgg tgagttttca ggcgcgagtt gggaaggaag aaggcatcgg ggatgaaagg
     9181 tgaaagtccc cccggccgcg cggcacgtgt ggccgggggg attgtaatga gctgccgggg
     9241 gcctcaggac acgagcatgc agcatcgatc cagacggcgg tttcggcgta ttatggcagc
     9301 ctgatcgcgc cggggcggga tgtgagtcct ggcgcatgac gaaatgtcgc gactggacgc
     9361 cgaaattcgc gtgtccggcg catacggtga taagacgtga acgaagaggg tgaaacgttg
     9421 tcccgctggg ccttctgggg tcatctcgcc gccgcggtat ggatcctcgg cacgactgtg
     9481 ctctatttcg tacgcctcag cgccgtgttc ttccgtgaca accacgacac cctgaacggc
     9541 ctgctggaac ggctcgggct tggttttctg gggtaaatgt gtgcctgccg ccgagaaacc
     9601 cggaacatcg acgcgcaaac aacgaaaacg aagcggagca tgcccgcaca gcgttttgtc
     9661 gtgagcgacg gggcatctgc tgcgcccgga cgcaccctaa atccggctca aaaccgggag
     9721 aaagaaagac tgcgtgaata tcgtggaggc tgtgtcatga tgcttcgagc agccggatta
     9781 ttggcgatgc tggttctgtg cgcgggcgtc gcatgcgccc aggctccgga gggcgaggcg
     9841 gaaaccatcg cggccctcgg cctggaccct ggg
//
LOCUS       DAOPBQ010000059         9625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6752_length_9625_cov_3.493730, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000059 DAOPBQ010000000
VERSION     DAOPBQ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6752_length_9625_cov_3.493730"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKW60_03255"
     CDS             complement(<1..410)
                     /locus_tag="PKW60_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98478.1"
                     /translation="MRQTRMGIALLLLSVVIPAWSAGAARLSPEGFALVDGEPRLLIG
                     SYHLPDDDAFLARLAGNGFNFVRGTLDRPVLDRIRAAGMYAWIPLGGALALTEGDTDS
                     AARLRQMIEEYKDHPALITWEGPDEALWMQWYSA"
     gene            complement(518..2062)
                     /locus_tag="PKW60_03260"
     CDS             complement(518..2062)
                     /locus_tag="PKW60_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPJ98479.1"
                     /translation="MLKLRLIALGLVAALTAAGVSADVTLHPLFSNHMVLQQGTRVPV
                     WGKAAPGETVSVTIGSDKESAKASEDGHWMVRLGRLEAGGPHTMTVTGENNELMVNDI
                     LVGEVWVASGQSNMAMTVKSSNNAEREIASADYPGIRLFQVPNKTADSPQEAVEAQWQ
                     LTTPETIPNFSAAAYFFGRELHQQLKVPIGLIHSSVGGTPSESWTSLDTLEATPEAQS
                     TLNRWDDILAKYPQALETYTKQMDDWKAAAEEAKKEGREAPARPREPLGPDSPFRPAS
                     LYNGMIAPLIPYAIKGAIWYQGESNVSRAYQYRTIFPAMIEDWRKHWGQGDFPFLFVQ
                     LANFTKREPEPGESRWAELREAQTMTLAVKNTGMAVIIDIGDAEDIHPRNKQDVGKRL
                     ACWALAKIYGQQRMASGPLYDSVKFRDGKAILRFDYAGNGLVAQGGELNGFAIAGADR
                     KFVWARAEISGKDNVTVWSDLVSEPVAVRYGWADNPECTLYNQAGLPASPFRTDDWPG
                     LTANSQ"
     gene            complement(2191..2883)
                     /locus_tag="PKW60_03265"
     CDS             complement(2191..2883)
                     /locus_tag="PKW60_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HPJ98480.1"
                     /translation="MLTGFITLLITDAMLLLVIQLVGALVRRHSREFPLHYAVLLLLF
                     CILAHYAATSVMAGGISTERDRWLTLAATFGCGFFPIALYVATVFHHPAVGGASARNA
                     SLDEARKQEAEGNVQESLRAYCQYLDGHPKELSIWFETADMLQRNGEFTFARNLLIKM
                     RDQFTDDDAATAKIMELWDTLPRPANSAVSGTGGAASDPNAVLQELIALRKQGAITEE
                     EYRRQKRELFRG"
     gene            complement(3025..5595)
                     /locus_tag="PKW60_03270"
     CDS             complement(3025..5595)
                     /locus_tag="PKW60_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPJ98481.1"
                     /translation="MALPIDPAQLGDILPRVERPSRYLGAELNAVHKEPGAVELRLAL
                     AFPDLYDLGLGNLGLLILYSILNELPWCWAERAYAPAADMEAALRARGLPLFSHESRT
                     PLGLLDGIGFTLQSELTYTNILNMLDLAQVPLRSTQRGEEAPLVFAGGPCVFNPEPLA
                     PFIDFFVIGDGEEAVVEVAQALRETRGRPRQNRLEALARLEGIYVPALYPTETLADGR
                     VLPARGAPPVRRRLIRDLNTAAYPTRLIVPFTQQVHDRAGLEVLRGCTHGCRFCQAGM
                     TTRPVRERDLSTLDTLLEGLIRETGYEETSLLSLSTCDYSRARELVDQAAARASTADM
                     SVSLPSLRLDTFAVELADKIADIRRSGLTFAPEAATPCLRAVINKWIPDESLFHAAEE
                     VFRRGWKRLKLYFMIGLPTETDDDVQAIIDLCTRTLSRVRAISRNAQLNTSISTFVPK
                     PFTPFQWARQIDLEEARRKQAMLRDGFSRHGALRFGRHHPFATFIEGLITRSDRRAAD
                     LLEAAWRHGARFDGWDEWRNEAAWELAIAETGASAKDALRARDRDERLPWDHIDALVS
                     KAWLWNDWQDALDLRHLDDCRGGDCHNCGVNETERELCRRMWMRAAEVQLERADTAMA
                     SPEPHAERAEPPAVQRLRFRIGRAGDARFLSNRELMTAWARALRRAGFPLSYSQGFHA
                     HPRITFSSAPALGEESECDYMDVVLRETLPPTEALERLTAALPMGLRVFQANVVPLKA
                     PSLMASVTGFSYTLVIHEPFEIIQARAEALMARSHIELDREGKMTRRGARRISTVDIR
                     PMIRRMAVEPGPEGAHAHVEVCLVDLRCVRMREILAMLATDPAVARVIKRATYLAEPA
                     "
     gene            complement(5608..6954)
                     /locus_tag="PKW60_03275"
     CDS             complement(5608..6954)
                     /locus_tag="PKW60_03275"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPJ98482.1"
                     /translation="MAETTDSARAITDLVEATPFVDTHEHLPEESWRLEALASPEGKK
                     GAVCDFSILFSHYADSDLVVAGLSLNDLNRICETTLPPKEKFRLLAPYYAAARHTGYL
                     RNVRESVRLLYGEDDLTEDNCERISERLAAGVKPGFYRHILKEVARVEYCQVNSLKSC
                     VFCETEMPELLAQDISTVPLCTGLDIVNVERLADREAYSLKDWKEIIDWCFATFGPRA
                     IATKNQCAYGRALDFEQVSEEDAAPLFERLLTKGPRDLSSGERKAIEDHLFHYCIDLA
                     AEYRLPVKIHTGYYAGHGNMPLSRVRANASDLCPVMQAHRNADFVLFHIDYPYQDEAI
                     ALAKHYPNAYIDMCWAWIINPQASIRFLKEYLTAAPANKLFTFGGDYLPVELVPGHAA
                     VARQGLAQAITGLVHEKWLDINEAPELVDRIMRGNAHALYRYEDTLKHWSKFQRTS"
     gene            7342..8427
                     /locus_tag="PKW60_03280"
     CDS             7342..8427
                     /locus_tag="PKW60_03280"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPJ98483.1"
                     /translation="MVELPQATLSQLLWGGGLFVGLILIAFSVERFRQCRRINLQRAA
                     EWNTVECIIRDKELTPEEQKALRALIRKQAPQDPLRVVTVRQYFDACVERFMASLGQP
                     RERKQYAEMGSLLRDVRVALALDYVPLGQRIQSTRELSPGQLISLALASDTTPRWHAG
                     RVDDLDEAFLYILLDDVEARSVNEFRPGVEVRARLWREDDARYAMMLECAETRETPHS
                     FAFYHSTKFDRLQARSDYRIRHEQTTSIGVLNRPLEDHQFDRLAERPLVTKLRGRITN
                     LSAGGCALVVQQPIPTQVLLRITIELDDWEPFDVFARVIATAPISGGRSFVRASFVAV
                     DDEMRDKIARYIIRRQQRLADDDEADE"
     gene            8405..9160
                     /locus_tag="PKW60_03285"
     CDS             8405..9160
                     /locus_tag="PKW60_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98484.1"
                     /translation="MTMKPTSDPSAIFPEAFRAVMRNPRALALYIVLAMGAAGAKLAA
                     MHLCTGGETLGNPGLTVRLLTLLTDLALVAIYAFAQTVAFSWLGQDVDRPLWRVKGLK
                     DALARFYSLWLILDLLIIALNSIAARQLNADATNALGHIMMLLFLSLQVISIPIGAAI
                     MFHGELKWRELGQSLAPLGRQFFATTLVVVFSGCTLVLLLFFLLPSSGSRLWLRPLVE
                     IPGGLAECIIFTATWRICIMDRDADTDDGYYEF"
     gene            complement(9294..>9625)
                     /locus_tag="PKW60_03290"
     CDS             complement(9294..>9625)
                     /locus_tag="PKW60_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPJ98485.1"
                     /translation="AIPVDKEDADVAMDAAQCIGCGACVAACPNGSAMLFVASKVSHL
                     AHLPQGRVEANRRVLNMVEQMDREGFGNCSNHYECEAACPKGISVEHIAKMNREHLRA
                     TFKPVGS"
BASE COUNT         1787 a         2902 c         3015 g         1921 t
ORIGIN      
        1 aacgcgctgt accattgcat ccagagggcc tcatccgggc cttcccatgt aatcagggcg
       61 ggatggtcct tgtattcttc gatcatctgc cgcaagcggg cggcgctgtc ggtatcgcct
      121 tctgtgaggg ccagcgcacc acccagaggt atccacgcat acatgcccgc ggcacgaatt
      181 ctatcgagca caggccgatc aagcgttccc ctgacaaagt tgaagccgtt gccggcgagg
      241 cgggcgagaa acgcatcatc gtccggcagg tggtaactgc cgatcagaag acgcggctcg
      301 ccgtcgacga gggcaaagcc ttccggggac agccgtgcag cgccggcgct ccaggccggg
      361 ataacaacgg aaagcagtag caatgcgatc cccattcgag tctgtctcat atcgggcgcc
      421 tccttcgaag cagcagatgc agcggctcat ccttattacg cgcgtgttca cggaattgcc
      481 cgtgagcgtg ccgaatcggc attcgttcgg aacaagttta ctggctgttg gcggtgaggc
      541 ccggccagtc gtctgtgcgg aacggcgagg caggcaaacc cgcctgattg tacagcgtgc
      601 attcgggatt gtccgcccag ccgtagcgta ccgccaccgg ttcactcacc agatcagacc
      661 acaccgtgac gttgtccttc ccagagatct ccgccctggc ccagacgaat ttccggtcag
      721 cgcctgcgat cgcgaaaccg ttgagctcgc cgccttgagc cacaagaccg ttgccggcgt
      781 agtcgaaccg gagaatggcc ttgccgtcgc ggaattttac ggagtcgtac aggggccccg
      841 atgccatacg ctgctggccg tagattttcg ccagcgccca gcaggcgagt cgtttgccga
      901 cgtcctgctt gtttctggga tggatgtcct ccgcatcgcc gatatcgata atgaccgcca
      961 taccggtatt cttgacggcc agcgtcatgg tctgagcttc acggagctcc gcccacctgg
     1021 actcccccgg ctccggttct cgtttcgtga agtttgccag ctgcacgaaa aggaagggaa
     1081 aatcgccctg tccccagtgt ttcctccagt cttcgatcat cgcagggaag atcgtgcggt
     1141 actgatacgc ccgggagacg ttggattctc cctggtacca gatggcgccc ttgatcgcat
     1201 acggaatcag gggcgcgatc atcccgttgt acagggaggc cggccggaag ggactatcag
     1261 gacccagcgg ttcgcgcggt cgtgccggcg cctccctgcc ttctttcttc gcctcctcgg
     1321 cggcggcttt ccagtcgtcc atctgcttcg tgtaggtttc gagcgcctgg ggatatttcg
     1381 cgaggatatc gtcccaacgg ttgagagtgg actgggcttc cggggtggct tccagggtgt
     1441 cgaggctggt ccaggactcc gacggcgtgc cgcccaccga cgaatgaatc aatccgatgg
     1501 gcaccttcag ctgctgatga agttcccgcc cgaaaaagta ggctgcagcg gaaaaattcg
     1561 ggatggtttc cggcgtcgta agctgccact gggcctcgac ggcctcctga ggactgtcag
     1621 ccgttttgtt cggcacctga aacaggcgga tgccggggta atccgcactg gcgatctccc
     1681 gctcggcgtt gtttgaactc ttgaccgtca tggccatgtt ggactgcccg gaagcgaccc
     1741 agacctcccc gacgaggatg tcgttcacca tcagttcgtt gttttcgcca gtcacggtca
     1801 ttgtgtgcgg gccgcccgcc tcaagccggc caagccggac catccagtga ccgtcttcac
     1861 tggccttggc actttctttg tccgaaccga tggttacaga aacggtctcg ccgggagcgg
     1921 ccttgcccca cacggggacc cgggtcccct gctgaagtac catgtggttt gaaaagagcg
     1981 gatgcaacgt gacgtcggca ctcactcccg ccgcggtcaa cgcggccaca aggcccaatg
     2041 caatcaggcg caatttcagc atgggattcc ctcccgggtt gtgcattcac cgaaaaagca
     2101 atgtaaatca tccagatcgg gtttggcaaa caccggacaa ccaccagcgc gttcatgaat
     2161 tcgccgcgtc agggtgctga gggtgagaag ttaccctcta aacagttcgc gtttttggcg
     2221 ccggtattcc tcttcggtga tggcgccttg tttccggagc gcgatcagct cctggaggac
     2281 ggcgttggga tcactggcgg caccgccagt gccggatacg gcggaattgg ccggccgggg
     2341 caaggtatcc cagagttcca taatctttgc ggtagccgca tcatcatcgg tgaactgatc
     2401 ccgcattttg ataaggagat tccgcgcaaa tgtgaattca ccattacgct ggagcatgtc
     2461 tgcggtttca aaccatatgc tcaattcttt gggatggcca tcgagatatt ggcaataagc
     2521 gcgcagagat tcctgtacat tgccttctgc ctcttgtttc cgggcttcat cgagtgaggc
     2581 gttgcgcgca cttgcgcccc cgacggccgg atggtgaaat acggtggcca catagagtgc
     2641 aatggggaaa aagccacatc cgaaggtcgc ggccagggtc agccagcgat cgcgctccgt
     2701 gctgatgccg ccggccatca ccgaggtcgc cgcgtaatgc gcgaggatgc agaaaaggag
     2761 caacaggacg gcataatgca gcggaaattc ccgcgagtgg cggcggacaa gtgcgccgac
     2821 gagttgaatg accagcagaa gcatcgcgtc ggtaatcagc agcgtgatga atcccgtcaa
     2881 catacgttca tccccctata gcgctcacac cactctatca cacgcgcgcc ctttgcccca
     2941 aaattccata aaacatcgca atgcaggaaa cattgggttt tgacatgcct cgaacccgcc
     3001 cgggatcaag gcgggctggc cggttcaggc aggctccgcg agataggtcg cgcgcttgat
     3061 cacacgggcc actgcggggt cggttgcgag catcgcgaga atttcacgca tcctgacgca
     3121 gcgcaaatcg accaggcata cctcgacgtg ggcatgggcg ccttccggcc cgggctcgac
     3181 cgccatgcgc ctgatcattg gccggatatc cacggtggaa atgcgccggg cgccccgccg
     3241 ggtcatcttg ccttcccggt cgagctcgat gtgggatcgt gccatcaaag cctccgcgcg
     3301 agcttgaatg atctcaaatg gttcatgaat cacgagggtg tatgagaaac cggtcacgct
     3361 cgccatcagc gagggcgctt tcaaagggac cacgttcgcc tggaaaacgc gcaaacccat
     3421 cggcagggcg gccgtcagac gctcaagggc ttctgttggg ggcagcgtct cacgaagcac
     3481 gacgtccatg taatcgcatt cggattcttc tcccagggcg ggcgcgctcg aaaacgtaat
     3541 cctggggtgg gcatggaatc cttgcgaata ggacagagga aacccggcgc gccgcaacgc
     3601 gcgcgcccag gccgtcatca attcgcggtt cgacaggaat cgcgcatcgc cggcacggcc
     3661 aattcgaaac cggagccgct gcaccgcggg aggctcggct ctctcagcat gcggttcggg
     3721 ggaagccatc gcggtgtctg cgcgttcaag ctggacctcg gcggcgcgca tccacatccg
     3781 gcggcagagc tcgcgctcgg tttcgttgac gccgcaattg tggcagtccc cgccccggca
     3841 atcgtccagg tgccgcaggt caagggcatc ctgccagtcg ttccacaacc acgctttgga
     3901 taccagggca tcgatatgat cccacgggag ccgttcatcg cgatcgcgtg cgcgcagcgc
     3961 gtctttcgcc gaggcgccgg tttccgcgat cgcgagttcc caggcggctt cgttgcgcca
     4021 ttcatcccac ccatcaaacc gggcgccgtg ccgccaggcg gcttcgagca ggtccgcggc
     4081 ccggcggtcg gagcgtgtga tgaggccctc gatgaaggtc gcgaacggat gatgccgccc
     4141 aaagcgaagc gccccgtggc ggctgaaccc gtcgcggagc atggcctgtt tgcggcgggc
     4201 ctcctcgaga tcgatctgcc gcgcccattg gaaaggcgtg aatggcttgg ggacaaaggt
     4261 ggagatgctg gtgttgagct gggcgttgcg gctgatcgcg cgaacgcggc tcagggtgcg
     4321 cgtgcagagg tctatgatcg cctgaacgtc gtcatcggtc tccgtgggca atccgatcat
     4381 gaaatagagc ttgaggcgtt tccagccgcg ccggaacact tcttcggcag catggaaaag
     4441 gctttcgtcg ggaatccact tgttaatgac ggcgcgcagg caaggtgtgg cggcttcggg
     4501 ggcgaaagtg aggccgcttc ggcgaatgtc cgcgatcttg tccgccagtt cgacggcaaa
     4561 cgtgtcgagg cgcagcgacg gcaaggacac gctcatatcc gcggttgatg cccgcgcggc
     4621 ggcctgatcc accaactccc gcgcccggga atagtcgcat gtggacagcg agagcaggga
     4681 tgtctcctca tacccggtct ctcgaatgag gccctcgagc agcgtatcaa gcgtggaaag
     4741 gtcccgttcg cggaccgggc gggtggtcat gccggcctgg cagaaccggc aaccatgcgt
     4801 acagccccga agtacttcca gcccggcccg atcatgcacc tgctgcgtga acggcacgat
     4861 cagtctcgtt ggataggccg ccgtgttgag gtcgcgaatc agccgcctgc gcaccggcgg
     4921 ggcgcctcgt gcggggagga cgcgcccgtc cgccagtgtt tcggtcggat agagcgcggg
     4981 cacatagatc ccctcgagcc gggcgagcgc ctcgagccgg ttctggcgcg gccggccccg
     5041 ggtctcgcgc agcgcctgtg ccacctcgac cacggcctcc tccccgtcgc cgatgacgaa
     5101 gaaatcgatg aacggcgcca ggggttctgg attgaacacg caggggccgc ccgcaaacac
     5161 cagcggggcc tcttcgccgc gctgggtcga gcgaagcggc acctgcgcca gatcgagcat
     5221 gttgaggatg ttggtatagg tcagttccga ctgaagcgtg aagccaatgc catcgagaag
     5281 gcccaggggc gtcctggatt cgtgcgagaa gaggggcaga cctcgagcgc ggagcgcggc
     5341 ttccatgtcg gcggcaggcg cgtacgcccg ctccgcccag caccagggaa gctcgttcag
     5401 aatggaatag agtatcagca gccccaggtt gccgagcccc aggtcgtaga gatcgggaaa
     5461 cgccagggcg aggcgcagct ctaccgcgcc cggctccttg tgaactgcgt tcagttcggc
     5521 gccgagataa cgggaagggc gctcaacgcg cggcaggatg tcgccgagct gtgcagggtc
     5581 tatgggaagg gccatgcgat cccgttccta agaggtccgt tggaatttcg accaatgctt
     5641 gagtgtgtct tcatagcggt acagggcgtg ggcattgcct cgcataatcc gatcgacgag
     5701 ttccggtgct tcgttgatat ccagccattt ctcgtgaacc agtcccgtga tggcctgcgc
     5761 cagcccctgg cgcgctaccg cggcatgccc gggcaccaac tcaaccggca ggtagtcgcc
     5821 tccgaaagtg aacagtttgt ttgccggggc cgccgtcaga tactccttca gaaaccggat
     5881 cgacgcctgc ggattgatga tccatgccca gcacatgtcg atgtaggcat tcggataatg
     5941 ttttgccagc gcgatggcct cgtcctggta ggggtagtcg atatgaaaga gcacgaaatc
     6001 ggcgtttcgg tgcgcttgca ttaccggaca caggtcactg gcgttggcgc ggacgcggct
     6061 gaggggcata ttgccgtggc cggcgtagta gccggtatga attttgacgg gcaggcggta
     6121 ctcggcggcc aggtcgatgc aatagtggaa aagatggtct tcgatggcct tccgctcccc
     6181 gctcgacagg tcccgcgggc ctttggtcag cagccgctcg aacaggggcg ccgcatcctc
     6241 ttcggacacc tgctcgaaat cgagcgcccg cccgtacgcg cactggttct tggtggcgat
     6301 ggcgcgtggt ccaaacgtcg cgaaacacca gtcgatgatc tctttccaat ccttaaggga
     6361 gtatgcctca cggtccgcaa gccgctccac attcacaata tcgaggccag tgcacagggg
     6421 gactgtgctg atgtcctggg cgagcagctc cggcatttcc gtttcgcaga acacgcagga
     6481 cttgagactg ttgacctggc agtattccac gcgggcgact tctttaagga tatgccggta
     6541 gaagccgggt ttgacgcctg ccgcgagccg ttctgagatg cgctcgcaat tgtcttcggt
     6601 caggtcgtcc tcgccgtaga gaaggcgcac cgactcccgc acattgcgca gatagccggt
     6661 atggcgcgcc gcggcgtaat acggtgcgag caggcggaac ttctccttcg gggggagcgt
     6721 ggtctcgcag attctattca ggtcgttcag tgacaaaccg gcgacgacga gatcggaatc
     6781 ggcatagtgg gaaaacagga tgctgaaatc acagacagcg cctttctttc cctccggact
     6841 ggccagcgct tcaaggcgcc acgactcttc ggggagatgc tcgtgcgtat ccacaaacgg
     6901 cgtagcctcg acgagatcag taatcgcccg ggcggaatca gttgtttcgg ccatgtcagg
     6961 ctgctctccc tggttttctg cgctgccggg gcaggggcac cgcgtgcacc agccggctgc
     7021 cacatcgcgc cattatccaa ttcctgccgc cacctctgca agcatccggg cggcccttaa
     7081 ttcgccgggc acatgcgtgc ctgtcgctcg agcgccgggc agtcggccca gtggacacga
     7141 caatacgggc tgtggaaatg tggaaaactc gctctgcgcc taacccggaa accacggcaa
     7201 cgtgacattt ctaacgagct aagacatgtg acatttttaa agagttttga caggaggcgt
     7261 tgagatgatt gcatcccgtt ccgagcaccc ctataatcag gcgcgcaaat attcagcatt
     7321 attctcagga agggcccttt catggtggaa ttgccgcagg ccaccctctc tcagctgctc
     7381 tggggaggcg gcctatttgt cggtctgatc ctcatcgcct tttcggtgga gcgttttcga
     7441 cagtgccggc gcatcaatct gcagcgtgcc gccgaatgga ataccgtgga atgcatcatt
     7501 cgcgacaaag agctcacgcc ggaagagcag aaggcgctgc gagcattgat tcgcaagcag
     7561 gcgccgcagg atccgctgcg ggtggtgacg gtacggcagt attttgatgc gtgtgtggag
     7621 cgtttcatgg cgagcctcgg ccaaccgagg gagcggaagc agtatgcgga gatggggagc
     7681 ctgcttcgag atgtgcgtgt tgcgctggcc ctcgattacg tgccgcttgg ccagcgcatt
     7741 cagtccaccc gcgaattgtc gccggggcag ttgatctcgc ttgcgctggc ttcggacacc
     7801 acgccccgct ggcacgcggg ccgtgtggac gacctggatg aagccttcct gtatattttg
     7861 ctggatgacg tggaagcgcg gagcgtgaac gagtttcgcc cgggcgttga ggtgcgcgcc
     7921 cggctctggc gtgaagatga tgcccgctac gcgatgatgc tggaatgtgc ggagacgcgc
     7981 gagacgccgc actcgttcgc cttttatcac agcacaaagt ttgaccggct tcaggcccgc
     8041 agcgattacc gcatccggca tgagcagacc acctcgatcg gcgtgctcaa caggccgctg
     8101 gaggaccatc aatttgaccg gctcgcagaa cgcccgcttg tgacgaagct gcgcgggcgc
     8161 atcaccaacc tgagcgcggg cggctgcgcg ctggtggtgc agcagccgat tcccacccag
     8221 gtgttgctcc ggatcacgat cgaactggac gactgggagc cgtttgatgt ttttgcccga
     8281 gttatcgcca ccgcccccat ttcaggcggg cgaagttttg tgcgcgcatc atttgtcgcg
     8341 gtggatgacg agatgcggga caagatagcg cgttatatca ttcgacgtca gcaacggctc
     8401 gccgatgacg atgaagccga cgagtgatcc ctccgcgatc ttcccggaag cgtttcgggc
     8461 agtaatgcgc aatccgcgcg cacttgcctt gtacatagtg ctggcgatgg gcgctgcggg
     8521 cgccaaactc gcggcgatgc atctctgtac cggcggagag accctcggca acccgggact
     8581 gaccgttcgg cttcttacgc tgctcactga tctggcgctg gtcgcgatct atgcgtttgc
     8641 gcagacggtg gccttttcgt ggctcggaca ggatgtcgac cggcctctgt ggcgggtgaa
     8701 agggctcaag gacgcgctgg cgcgcttcta ttcgctctgg ctcatactgg atttgctcat
     8761 catcgcgctc aacagcatcg cggccaggca gctcaatgcc gatgccacca atgcgctggg
     8821 ccacatcatg atgctgctgt ttttgagcct gcaggtcatc tcgatcccca tcggagcggc
     8881 catcatgttc cacggagaat tgaaatggcg tgagctgggc cagagtctgg cgcctctcgg
     8941 ccggcagttt tttgcgacca ctctggtggt ggttttcagc ggctgtacgc tggtgctgct
     9001 gttgtttttt ctgcttccct cctcaggcag ccggctctgg ctccgcccgc tcgtcgaaat
     9061 cccgggcgga ctggcggaat gtattatttt cacggccacc tggcgcatct gtatcatgga
     9121 ccgcgacgca gacacggacg acggctacta cgagttctga ggctccatgg acacggctat
     9181 gcgggttgag tttccctatg ggccaggcat cctcacgggc agcctctccg gaacgcgatg
     9241 cctggttcgt gactgacctg ccgccggatg atgtcggcgc actgcatgcc cgttcaactt
     9301 ccgacgggtt tgaacgtagc gcgcaggtgt tcgcggttca tcttcgcgat gtgttcgacg
     9361 ctgatcccct tggggcaggc tgcctcgcat tcgtagtggt ttgagcagtt gccgaagcct
     9421 tcgcgatcca tctgttcgac catattgaga acgcggcggt tcgcctcgac ccgtccctgg
     9481 ggcagatgcg cgaggtggct gactttcgac gcgacgaaga gcatcgcgga gccgttcgga
     9541 cacgcggcga cacaggcgcc gcacccgatg cactgggcgg catccatggc aacgtcggca
     9601 tcttctttat ccacggggat ggcat
//
LOCUS       DAOPBQ010000060         9498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6849_length_9498_cov_3.824526, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000060 DAOPBQ010000000
VERSION     DAOPBQ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6849_length_9498_cov_3.824526"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PKW60_03295"
     CDS             complement(<1..589)
                     /locus_tag="PKW60_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPJ98486.1"
                     /translation="MTSVHKIRNVAIIAHIDHGKTTLIDSIFRAARVFRENAQVAERV
                     MDSNELERERGITIRAKHCTVEWNGYRVNIIDTPGHADFSGEVERILSMVDSVLLLVD
                     ASEGPMPQTRYVLMRALRQGLRPIVIVNKADRPSANPARTLDLTFDLFVELGANDTQC
                     DFPVLYGSGLQGWLVRDLQTDPHAGMDALFETIIAH"
     gene            complement(719..1939)
                     /locus_tag="PKW60_03300"
     CDS             complement(719..1939)
                     /locus_tag="PKW60_03300"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPJ98487.1"
                     /translation="MNFEELNIDPRCLRILKSQHINEPTPVQAQAIPIALEGRDLVGI
                     AQTGTGKTLAFALPSLARLANDPSGKAGMLVLTPTRELAQQVHAVLDPLARAMGLRTA
                     CIFGGVGFDAQTRALRQKCPVIVATPGRLLDHMGRKNTNFEHLAVLVLDEADRMLDMG
                     FLPDIQRILGGLPRERQTLMFSATFPKSIERLAAEMLHQPARISVGAAATPAAAIRQG
                     IYTVEHGRKLGLLSKLLSEPGVDSALVFLRTKHRTDRTARALQKAGFKAKAIHGGRTQ
                     SQRDQAIEGFRKGRYKVLVATDVAARGLDVEGISHVVNFDIPNNPEDYIHRIGRTARA
                     NASGDAITFVCPDEFLALNEIETALGRNLPREEWEGAVPVVSLFNGDNHGTLKNKRKG
                     FRNARRGRRLMAVR"
     gene            complement(2289..4214)
                     /locus_tag="PKW60_03305"
     CDS             complement(2289..4214)
                     /locus_tag="PKW60_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPJ98488.1"
                     /translation="MNNNNHTPNNTNGTRTLGPVSDLERHLPSDWWRTLFKALYLKTD
                     GDVVENETNTIRDVDQLIMATGIGLDDRILDLCCGQGRHSMELARRGYKNVTGIDRSR
                     HLVRLARRRAERKKLQITFREGDARKFRLPESSFDCVFIMGNSFGYFEREEDDLAVLR
                     AVLRVLKTGGRVLLDVTDGDWMRSHFEPRSWEWVDQTHFVCRERALAGDRVRLISREV
                     VTHVERGVIADQFYAERLYTCDDLKRLLRTAGFVDVSRVAAPVTGSDRQQDLGMMAQR
                     IFIVATVPQSRVKLRRRGPLFRDVTVLLGDPRLPDDVKREGQFNPEDMDTVRRMQDAL
                     GTLREYNFAYWDDHGSLLHFLEQERPAFVFNLCDEGFKNDPFKELHVPALLELFGIPY
                     SGGAPACLGMCYNKALVRSIAQSLNIPVPLETYSGPDDQAATLPSLFPALLKPNFGDS
                     SQGITKDAVVHTKEELIDYLESLRRDFPRRPVLVQEFLTGTEYSVGVIGNPGLDVTVL
                     PMLEVDYSGLPQGLPPILGYESKWHPDSPYWNEIRYHEARISETVKRALNDYSLALFE
                     RLECRDYARFDFRADAQGRIKLLEVNPNPGWCWDGKLNFMAGFAGLGYPELLQLIIEA
                     AQSRYQAAGALAETVYA"
     gene            complement(4728..4814)
                     /locus_tag="PKW60_03310"
     tRNA            complement(4728..4814)
                     /locus_tag="PKW60_03310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4778..4780),aa:Leu,seq:cag)
     gene            5088..5813
                     /gene="recO"
                     /locus_tag="PKW60_03315"
     CDS             5088..5813
                     /gene="recO"
                     /locus_tag="PKW60_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPJ98489.1"
                     /translation="MSQERTEAVVLRGVDFSETSRIVTFLTPDRGKVACMAAGARRPK
                     SNLSGVLDVFNRLEIVYYWKDGRSVQRLAEASVLETYPAIKADLERSVCAAFPLEIVY
                     KAAQENEPSEQLYSTLVRGLTSLTQWTGDSRTHTAWQALQLMSSAGFEVTLAPRGTRE
                     WVWFSYEHGVLSPAQRGDRRLNALEYDGLCALAAHPESCPDIEGITAVFAAVRGYVER
                     HLEAGFRSVRVIDQVFDRTRRQV"
     gene            5847..7661
                     /gene="lepA"
                     /locus_tag="PKW60_03320"
     CDS             5847..7661
                     /gene="lepA"
                     /locus_tag="PKW60_03320"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011887843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPJ98490.1"
                     /translation="MATPQQQIRNFSIIAHIDHGKSTLADRLLQYTGAVDARNLREQT
                     LDTMDLERERGITIKSVAARLNYRAQDGLDYVLNLIDTPGHVDFSYEVSRSLAACEGA
                     LLLVDAAQGVEAQTLAHAYRAIEQNLEIIPVINKVDLPSADVESTRHQIEDVVGLDAS
                     EAVLASAKSGLGTREILEAIVKRVPPPQGQRSGPPRALIFDAKYDPYRGVVTYVRVVD
                     GRLVKNQRIMLMSNGERYEISELGFLTPEMRPAEALEVGEVGYLICGIKTLATVNIGD
                     TVTDASRPAGAPLPGYKEIQPVVFCGMYPAGTTDIQELRDALDKLHLNDASFRYQADN
                     SDALGLGFRLGFLGLLHMEIVQERLEREFDLTLVTTVPNVEYHAHQTDGQMRVVQSAS
                     QMPDPARIDRVEEPYIEVEIITPTEYLSPIIELCKKKRGLHVRVDYLDARRCLAVYQM
                     PLAEIVLDFYDKLKTYSRGYASLEYHMIGYREGDLVKLDILLNGEPVDALSTVVFRGS
                     AEWIGRTLASKLRKLIPRQQYEVAIQAAIGNRILVRETVKALRKNVTAKCYGGDISRK
                     RKLLEKQKEGKRRMKQVGTVEVPQEAFMALLQVGDEGR"
     gene            7667..8974
                     /gene="lepB"
                     /locus_tag="PKW60_03325"
     CDS             7667..8974
                     /gene="lepB"
                     /locus_tag="PKW60_03325"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPJ98491.1"
                     /translation="MAGSGGSRQRAEDPRGVFSRLRDAITFITGPWTLENGKAWLKVI
                     VVVLLIRWLWFEPFSIPSGSMEPTLHGDAKFLKGDRVAVNKLAYGPRVPFCNIRLLDM
                     GDPARWDIVVFITPEKGAAHKVLIKRVAGLPGERIHISNGQLHVNGQAVPFPADMPAE
                     MRYTNELEDLERGRRLYMSSEHMRYGVLPQDEYSLIPEGHYFLLGDNSARSRDGRYFG
                     WVPEENIVGRAFCIWWPWGHRRDFTGFSRTVWGKGILFGIPAAVLLYLFVTGFLVMSM
                     RVRHGQVPDFPARGERVLINRLMAGLRVPFTNARLFPGRLPRRGEVIAYAAEDGDNSS
                     PQMFLGRVVGTPGQNVSRRDGSCCIEGHVVGSLADEGKFKPGRVPEGGVVVVYDAADK
                     RAAGPPVAWVSREDIIGSVVSVWWPPHRARSVGGGAEPPVGAG"
     gene            8991..>9498
                     /gene="lepB"
                     /locus_tag="PKW60_03330"
     CDS             8991..>9498
                     /gene="lepB"
                     /locus_tag="PKW60_03330"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPJ98492.1"
                     /translation="MAGRGKKRENIQPGGKPSRGAQAAKAGLGALLVGALAWLTGGFT
                     RESAIEWAKTILVAGSLALAIRWSVAEPFRIPSGSMEPTLHGDPHFLLGDRVFVNKWR
                     YGLRYPFMKKRIYYGESPKRWDIVVFKSAEQDATQTTLIKRIAGLPGERVHIQDGKLY
                     VNGEPLPFP"
BASE COUNT         1793 a         2631 c         3230 g         1844 t
ORIGIN      
        1 catgcgcaat gatggtctcg aagagcgcat ccatcccggc atgggggtct gtctggaggt
       61 cgcggacgag ccatccctgc aggcccgacc cgtagagtac cgggaaatcg cactgcgtgt
      121 cgttcgcgcc gagctcaaca aaaagatcaa aggtgagatc gagcgtgcgt gcggggttgg
      181 cactgggccg atcggccttg ttgacgatga caatgggccg caatccctgg cggagggcgc
      241 gcatgagcac atagcgggtc tggggcatgg gcccttcgct cgcgtcgacc agcaggagca
      301 cggaatcgac catggagagg atgcgctcaa cctcgccgga gaagtcggca tggcccggcg
      361 tgtcgatgat attgactcgg tagccgttcc attcaacagt gcaatgcttg gcccggatgg
      421 tgatgccgcg ttcgcgttcg agctcattgc tgtccatgac gcgctcggct acctgggcgt
      481 tttcgcgaaa aacccgggct gcacggaaga tgctgtcgat cagggtggtt ttcccgtgat
      541 cgatatgggc aatgatggcc acgtttctga ttttgtggac actggtcatg cgcctgggtt
      601 ccttgctggt ttcagactat ccggccggtc agccgcgctg taagaagaaa ggcggcggag
      661 tgtaactcat gttccggcct ccacacaaat tcacgatctg ctggatgtgc cgggcggatt
      721 accggaccgc catcaggcgc cggccacggc gggcgtttcg gaagcctttc cgtttattct
      781 tgagcgtgcc gtgattatcg ccattgaaca gcgacaccac ggggacggcc ccttcccatt
      841 cctcgcgggg gaggttcctg ccgagcgcgg tttcgatctc gttgagggcg agaaattcgt
      901 cgggacagac gaatgtgatg gcgtcgccag aggcgttggc gcgcgcggta cgtccgatcc
      961 gatgaatgta gtcctccggg ttattgggaa tgtcaaaatt gaccacgtgg ctgatgcctt
     1021 cgacatccag cccacgggcg gccacatcgg tcgccacaag aaccttatat cgtcctttgc
     1081 ggaatccttc gatggcctga tcgcgctggc tctgggtgcg gccgccgtgg atggcttttg
     1141 ccttgaaccc tgctttctgg agtgcgcgcg cggtgcggtc ggtacggtgt ttggtccgga
     1201 ggaataccag ggccgaatcc accccgggct cactgaggag cttggagagg agtccgagct
     1261 tgcggccatg ctccacggtg tagatgccct ggcggatggc tgctgcgggc gtagcggcgg
     1321 cacctacgct gatccgggcg ggctggtgca acatctcggc agccaggcgc tcgatcgatt
     1381 tggggaaggt tgccgaaaac atgagcgtct ggcgttccct gggcaggccg ccgaggatgc
     1441 gctggatgtc gggcaggaag cccatatcga gcatccggtc cgcctcgtcc aggacgagca
     1501 cggcgaggtg ttcaaaattg gtgttcttac ggcccatatg atccagcagg cgtcccggcg
     1561 tggccacgat aaccgggcat ttctggcgaa gggctctggt ttgggcatca aatccgacgc
     1621 cgccgaaaat acaggctgta cgcaggccca tggctcgagc caggggatcg agcacggcgt
     1681 gcacctgctg ggccagctcg cgggtggggg tgagcacgag catgcccgcc ttaccgcttg
     1741 ggtcgttcgc gagacgggcc aatgacggca acgcgaacgc gagcgtcttg ccggtaccgg
     1801 tttgtgcgat gccgaccagg tcgcgccctt cgagcgcgat ggggatcgcc tgggcctgca
     1861 ccggcgtggg ttcgttgatg tgctggctct tcaaaatgcg aaggcagcgg gggtcaatgt
     1921 tcagttcttc aaaattcact gtgatcttcc ttcttccctg ccggtcagta ccgttcgagg
     1981 gagtattctg ctttttcagc accggcaaag caggaagcat cacgcatgtg ggtccgactc
     2041 tgaggtgagc aaagtgtagg agcgagcgtc gacgttaaac acaccactac aattaagcag
     2101 gtcttacatt gaagcaaagt atacaccata gcgcggcgag atgcaaaatg gccgctgtca
     2161 agacactgaa acaacgcaga tccgagactg tcaggcgcgc aggaagcccc aaggtgatgt
     2221 ttgcccggag cgggcgcacc ggcgtctgta aagcaagcaa gcgaagcgct gagccggcgc
     2281 ccggcgcctc acgcgtagac ggtttcggcc agggcgccgg cggcctggta acggctctgg
     2341 gcggcctcga tgatgagctg gagcagctcg gggtagccca ggccggcgaa tcccgccatg
     2401 aagttgagtt tgccgtccca gcaccagccg gggttggggt tgacttcgag cagcttgatg
     2461 cggccctgtg cgtcggcgcg gaaatcgaac cgggcgtagt cgcggcactc gaggcgctcg
     2521 aacagggcga gggaatagtc gttcagggca cgtttgacgg tttcgcttat gcgggcctcg
     2581 tgataacgga tctcgttcca gtagggcgag tcggggtgcc atttggactc gtagccgaga
     2641 atgggcggca atccctgggg caggccgctg taatcaacct cgagcatggg cagcacggtg
     2701 acgtcgaggc cggggttgcc gatgacgccg acgctgtatt cggtccccgt aagaaattcc
     2761 tgaaccagca ctgggcgccg tggaaagtcg cggcgcaggc tttcgaggta gtcgatcagt
     2821 tcttccttgg tgtgcacgac ggcgtctttc gtgatgccct ggctgctgtc gccgaagttt
     2881 ggtttgagga gcgcgggaaa cagcgacggc agggtcgcgg cctggtcgtc ggggccggaa
     2941 taggtctcga ggggcacggg tatgttgagg gactgagcga tcgagcgcac gagggccttg
     3001 ttgtagcaca tgccgagaca ggctggcgcg ccgccggaat aggggatgcc gaacagctcg
     3061 agcagggcag gcacatgcag ctctttgaac gggtcgttct tgaatccctc atcgcacaaa
     3121 ttgaacacga acgcggggcg ttcctgctcc agaaaatgca gcaggcttcc gtgatcatcc
     3181 caataagcga aattgtattc gcggagtgtg cccagggcat cctgcatccg gcgcacggta
     3241 tccatgtcct ccggattgaa ctggccttcg cgcttgacgt cgtcgggcag gcgcggatcg
     3301 cccaggagca cggtcacgtc acggaacagg ggtcccctcc gccggagctt cacccgcgac
     3361 tgaggcacgg tggcgacaat gaagatgcgt tgcgccatca tcccgaggtc ctgctggcga
     3421 tcggaaccag tgaccggggc ggcgacgcgg ctgacgtcga cgaaccccgc ggtgcgcagc
     3481 aggcgcttga gatcgtcgca ggtgtacagg cgttcggcgt aaaactgatc cgcgatgaca
     3541 ccgcgctcga cgtgggtcac gacttcacgg ctgatcaggc gcacccggtc gccggccagg
     3601 gcgcgttcgc ggcagacaaa atgggtttga tcgacccact cccagcttcg gggctcaaaa
     3661 tgggagcgca tccagtcgcc gtcggtgacg tcgagcaaga ccctgccgcc ggtcttgagc
     3721 acgcgcagca cagcccgcag cacggcgagg tcatcttctt cgcgctcaaa atagccgaag
     3781 gagttgccca tgatgaagac gcaatcgaag gacgactcgg ggagccggaa cttgcgcgcg
     3841 tcgccttcgc ggaaggtgat ctggagcttt ttgcgttcgg cgcgccgccg ggccagccgg
     3901 acgaggtggc gagaacggtc gatgccggtc acgtttttgt agccgcgccg cgcgagctcc
     3961 atgctgtggc gtccctgccc gcagcacagg tcgagaatac ggtcatccag cccgattccc
     4021 gtggccatga tgagttggtc aacgtcccga atggtgttgg tttcattctc gacgacatcg
     4081 ccgtctgtct tcaagtagag ggctttgaac agggtgcgcc accagtcgct ggggagatgc
     4141 cgttccaggt cagatacagg ccccagcgtg cgcgtgccgt tggtgttgtt cggtgtgtga
     4201 ttgttgttgt tcatgtccga gcttccttaa cccttcggtg tgattgggtc gcgtgccatt
     4261 gccaggaatt cgtcttgtcg gattggattt gatgcatgtc ccgggacggg accgagcgtt
     4321 tcggcctaat gcggcatctc aatgaagatg aaatggtggg tgaactgggc aggcgcggca
     4381 cagcggccgc tggcagtgtc tgggcagaat ggcccgtgtc gatggcttgg attttgtgcg
     4441 tccatccagg ctctccggat atccggcatg ccggtaatct gttgtgagat tgacacttgc
     4501 gtactttccg cgagccaaat catacctgtg ctctgggtgc gttgcacttc cagaatcgga
     4561 aatagtatac accgatgcct cccgttgtga aacaggtttt tgcgcgtatg gagggaggcg
     4621 ggggaaatgg gttgcgcagc gaagccgcac gcgccggcgg ctggccgtga ccggggcaat
     4681 gaaaaacgcc cggcccggtg aacccgggcc gggcacattg atggccatgg tgccggagaa
     4741 gggacttgaa cccccacgcc cttgcgagca catggtcctg aaccatgcgt gtctaccaat
     4801 tccaccactc cggcggcatg cttcgcttga cgaagtgaac aaatcttaac gcatggaagt
     4861 ggctcaggtc aaatcggcga atcggctggc cattgggcca gtcttttcgc agtaaactgg
     4921 ccggcacagc agcagcgtcc gctgtgccgg acggcgggcc agtttgcttc cccgtcgatg
     4981 gagtggtaag atagcgccgg taatcccttg cccgccttgg acttcgtttt gcctcccctt
     5041 cgagccgggc aatggatgta tacggtccgg agcgtcggag tggacttgtg tcgcaggaac
     5101 gaacagaggc cgtggtgctt cggggcgtgg acttcagcga gacgagccgg attgtgacat
     5161 ttctgacgcc ggatcggggc aaagtggcgt gcatggccgc gggagcgcgc cggccgaaga
     5221 gcaatctgtc gggcgtgctg gatgtgttta accggctgga gatcgtttac tactggaagg
     5281 acggccggtc ggttcagagg ctggcggagg cgtcagtcct ggagacctat cccgcgatca
     5341 aagcagatct tgagcggtcg gtatgcgcag cgtttccact ggagatcgta tacaaggcgg
     5401 cccaggaaaa tgagccgtcg gagcagctgt actcgacact cgttcgcggg ctgacttccc
     5461 tgacgcaatg gacgggcgac agccggacgc atacggcgtg gcaggcgctt cagttgatgt
     5521 cgagcgcggg ctttgaagtg actctggcgc cgcgcggcac tcgcgagtgg gtgtggtttt
     5581 cgtacgaaca cggcgtatta tcgccggcac agcggggtga tcgccggctg aatgcgctcg
     5641 agtacgatgg gttatgcgca ctggccgcgc acccggagtc atgcccggac attgaaggaa
     5701 tcaccgcggt gttcgcggcg gtccgcggtt atgtggagcg gcatctggag gcaggtttcc
     5761 gcagtgtacg ggtaatcgac caggtatttg accgaacacg acgtcaggtt tgagaaatcg
     5821 agagaattcc gccggataca ggttacatgg caacaccgca gcagcagatt cgcaattttt
     5881 cgatcatcgc ccacatcgac cacggcaagt cgacgctggc ggaccgcctg ctccaatata
     5941 cgggagcggt tgacgcgcgc aatctgcgcg aacagacgct cgacacgatg gatctggagc
     6001 gtgagcgggg gatcaccatc aagtcggtcg ctgcccgtct gaactaccgg gcccaggatg
     6061 gcctggacta cgtgttgaat ctgattgata cgccggggca cgtggatttt tcgtatgaag
     6121 tgtcgcgcag cctggccgcc tgcgaaggcg cgctgctgct ggtggatgcc gcccagggag
     6181 tagaggcgca gaccctggcc catgcgtacc gggccattga gcagaatctc gagatcattc
     6241 cggtgatcaa caaagtggat ctccccagcg cggacgtgga atcgacccgg catcagatcg
     6301 aagatgtggt ggggctcgac gcaagcgagg ccgtgctggc aagcgccaaa tcaggcctgg
     6361 gtacccggga gatcctcgag gcgattgtga aacgggtgcc gccgccacag ggccagcgca
     6421 gcggcccgcc acgcgcgctg atcttcgatg ccaaatacga tccctaccgc ggcgtggtga
     6481 cgtacgtgcg tgtggtggac ggccgcctcg tgaagaacca gcggatcatg ctgatgtcga
     6541 acggcgagcg gtatgagatc tccgaactgg gctttttgac gccggagatg cgccccgccg
     6601 aggcgttgga ggtgggagag gtaggttacc tcatctgcgg gatcaagacg ctcgcgacgg
     6661 tgaatatcgg ggacaccgtg acggacgcgt cgcgccccgc cggagctccg ctgccggggt
     6721 ataaggaaat tcagccggtg gtgttctgcg gcatgtatcc cgcgggcacc acggatatcc
     6781 aggaacttcg tgacgcgctg gacaagctgc acctgaatga cgcctcgttc cgatatcagg
     6841 cggacaactc ggacgcgctg ggtttggggt ttcggctggg attcctgggc cttctgcaca
     6901 tggaaattgt gcaggaacgg ctggaacgcg aatttgatct gacgctcgtg accacggtcc
     6961 cgaacgtcga atatcacgcc caccagacgg atgggcagat gcgggtggtc cagagcgcat
     7021 cgcagatgcc cgatcccgcc cggattgatc gcgtggaaga gccctacatc gaggtggaga
     7081 tcatcacccc cacggagtat ttgagcccga tcatcgagtt gtgcaagaaa aagcgggggc
     7141 ttcatgtccg cgtggactac ctggacgccc ggcgctgcct ggcggtgtat cagatgccgc
     7201 tggcggagat cgtgctggac ttctatgaca agttgaagac ctacagccgg ggatatgcct
     7261 cgcttgaata tcacatgatc gggtaccgtg aaggcgactt ggtcaaactc gacatcctgc
     7321 tcaatgggga gccggtggac gcgctgtcga cggtcgtttt tcgaggctcg gcggaatgga
     7381 ttggacggac tctggcctcg aaattgcgca agctgatccc gagacagcag tacgaagtgg
     7441 ccatccaggc ggcgattgga aaccgtattc tggtgcgcga aaccgtcaag gcgctgcgca
     7501 aaaacgtcac agcgaagtgc tacggcggcg acatttcacg caaacgcaag ctgctggaga
     7561 aacagaagga aggtaagcgc cgaatgaagc aggtcggtac ggttgaagtg ccccaggagg
     7621 ccttcatggc gctgctgcag gtcggcgacg aagggcggta gcaggcatgg caggatcggg
     7681 tggcagtcgg cagcgcgcgg aggatccccg gggcgttttc tcccgtctcc gcgatgcgat
     7741 cacgtttatc accggcccgt ggacgttaga gaacgggaaa gcctggctga aagtgatcgt
     7801 ggtcgtcctg ctgatccgct ggctgtggtt cgagccgttc agcataccgt cggggtcgat
     7861 ggagccgacg ctccacggcg atgcgaaatt cctcaaaggc gaccgggtgg cggtgaataa
     7921 actcgcatac ggtccgcggg tgcctttctg caacatacgt ctgcttgata tgggcgaccc
     7981 agcgcgctgg gacattgtgg ttttcattac gcccgaaaaa ggcgctgcac acaaggtgct
     8041 gatcaagcgg gtggccggat tgccgggcga gcggatccac atttcgaacg ggcaactcca
     8101 tgtaaacggc caagcggtgc cgtttcccgc ggatatgccg gcggagatgc gctatacgaa
     8161 cgaactggag gacctggagc ggggcaggcg cctctatatg tccagcgaac acatgcgcta
     8221 tggcgtgttg ccgcaggatg agtacagcct catcccggaa gggcactatt tcctgctggg
     8281 cgacaacagc gcccggagcc gcgacgggcg atatttcggc tgggtgcccg aagagaatat
     8341 cgttgggcgg gccttctgca tctggtggcc atggggccac cggcgcgatt tcacgggatt
     8401 ctcccgcacg gtgtggggga agggaatcct gtttgggatt cccgcggctg tcctcttata
     8461 cctgttcgtg accgggtttt tggtgatgtc gatgcgggtc cggcatggcc aggtgcccga
     8521 tttcccggca cgcggcgagc gcgtgctgat caaccgcctg atggctggat tgcgcgtgcc
     8581 gtttacgaat gcacgcttgt ttcccgggcg cctgccgcgc cggggggaag tgatcgcgta
     8641 tgccgccgaa gacggggata actcgtctcc gcagatgttt cttggccgcg tggtgggcac
     8701 gccgggtcag aacgtctcgc gcagggatgg cagctgctgt atcgaggggc atgtcgtggg
     8761 ctcgcttgcc gacgaaggga aattcaagcc tgggcgcgtg cccgagggcg gggtagtggt
     8821 cgtgtatgat gctgcggaca aacgagcagc tggaccgccg gtagcgtggg tctcgcgaga
     8881 ggatatcatc ggcagcgtgg tgtcagtgtg gtggccgccc catcgagcgc gcagcgtagg
     8941 gggaggggca gaaccgccgg ttggcgcggg ttgaacggaa gggagaccgg gtggcaggcc
     9001 gcgggaaaaa gcgagagaac attcagcccg gagggaagcc gtcgcgcggc gcccaggcgg
     9061 cgaaggcggg tctgggggcc ctgctggtcg gcgcgctggc ctggctgacg ggcgggttta
     9121 cgcgcgagag cgccattgaa tgggcaaaga cgatccttgt cgcgggttcg ctggctctgg
     9181 cgattcgctg gtcggtcgcg gaaccgttcc gtattccatc gggctccatg gagcccacac
     9241 tgcacgggga tccccatttt ctcctgggcg accgtgtttt cgtgaacaaa tggcgctacg
     9301 gactgcgcta tccgttcatg aaaaaacgga tctattacgg cgaatctccg aaacgctggg
     9361 acatcgttgt tttcaaatcc gcggaacaag acgcgacgca aacgacgctg atcaaacgga
     9421 tcgccgggct tcccggcgag cgtgttcaca tccaggacgg aaaactgtac gtgaatggag
     9481 agccgctgcc gttcccac
//
LOCUS       DAOPBQ010000061         9448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6889_length_9448_cov_4.448206, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000061 DAOPBQ010000000
VERSION     DAOPBQ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6889_length_9448_cov_4.448206"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /gene="rpsR"
                     /locus_tag="PKW60_03335"
     CDS             complement(<1..204)
                     /gene="rpsR"
                     /locus_tag="PKW60_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPJ98493.1"
                     /translation="MARAMGKRSKKRRKKKRGTGRAKVCRLTVDRVVYIDYKDVNLLK
                     HYVTERGKIIPRRITGATAQHQRM"
     gene            complement(238..663)
                     /gene="ssb"
                     /locus_tag="PKW60_03340"
     CDS             complement(238..663)
                     /gene="ssb"
                     /locus_tag="PKW60_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPJ98494.1"
                     /translation="MSDLRMPDLNKVFLAGRLTRDPELRYLSNGTPLCRMGLAVSRNY
                     KTREGERREETLFVDVVAWRGTAEYCGENLKKGRPILVEGMLRSDQWEDKATGQKRSK
                     IEVQADRIQTLAWDSDGGTGGKPVPRVIEEPIPEDDVPF"
     gene            complement(691..1203)
                     /gene="rpsF"
                     /locus_tag="PKW60_03345"
     CDS             complement(691..1203)
                     /gene="rpsF"
                     /locus_tag="PKW60_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPJ98495.1"
                     /translation="MRTYEALYIVHPEVKDDEIQTIAKNVETLVTDNGGAIVRSEIQG
                     KRKLAYEVKRCTEGCYVLLRFQAEPPFITKLETYFRLTEAIIRSLVVYFDEHTLRLEA
                     EQEKRRQLELQAGAAARSLDDEEDDDDDRGRRRRSSRYRDDDDDDDDDDDRERGRRRR
                     PARSAASDDD"
     gene            complement(1435..3924)
                     /locus_tag="PKW60_03350"
     CDS             complement(1435..3924)
                     /locus_tag="PKW60_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98496.1"
                     /translation="MRYGRGCRTWWFSAGALLLLAAVLAASPDAIGAEPDSDTAADRM
                     ERGIPGPLPGHPGNVFLTGEAVEIPGPPDTAWTQFRILDDAGETQGGGPRVNGATQAA
                     TGALPAGWYRIEFLDAGGKAVARTSAAVLAPLIVPIPPDSPICIDTAMAWFARKDAAK
                     QRAFASLSALAGMNWSRDRLSWGELETAPGVFAQDSTSYDTSASAAAAEGLNVLQVFH
                     QTPQWAQNTELDSTSPGGRFPRDLRVLYRFCNALAKRYQGRVQAWEPWNEANITSFGG
                     HLINEMCSFQKAAFLAFKAGDPELTVCWNVYAGSGSALHTEGVLLNEAWPYFDTYNIH
                     SYSAPADYLTEFEGPRNAASGKPIWLTECGIRLKAAEPEPAEDMSDEDELRQAWFVAP
                     SYASSLYSGVERHFFFILGNYLERGIQFGLLRDDLTPRPGYAALAAVGRLLAGARPLG
                     RLCIDAEANAWAYAFHAQPEGYPVEVLVAWADSAFSWQPVFARAPVAVHDYLGRARVD
                     GTKLELGPAAVFVVLPEGTCGQLALEPPPPLAMLREGTPSPVVLQVEFPQEAVRLESQ
                     AYELAAGEPSAMPLWVYNFSAEPVEGRLAVDSAPVDWRVELPGGLIQIPPLDGVPVTL
                     ELALPTNGRPALVGDWVHIRGDFGSAGRPVLAFRLIGERDSLVPRHVHSIEGAADPAR
                     WEDNIVAGASMSHQVAEGGTQVFTMQFGDGEPWSYPRLRLEPSERPSADDDGLAVTVG
                     LIEGEGTVNVQFIEESGATYVARLGAPSAGDGPQRLMALFSEAYWATYSRPDPNGRLD
                     PGQISIILIGLSSRRNTRAELAIAEPAWVRY"
     gene            complement(3951..4841)
                     /locus_tag="PKW60_03355"
     CDS             complement(3951..4841)
                     /locus_tag="PKW60_03355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPJ98497.1"
                     /translation="MFIDIHAHTSPRRHPKITRVNGSHYPEPARLIQMMDAAHIDKAC
                     IMGGVSPECRYTVVTVEDVVELCAQYPERLIPFCNLDPRYLTNDASADFSDLLSAYKD
                     LGCKGVGECTFNLPFDDPRCLNLFEAVEKASLPLTFHIGPEIGGCYGIFDDPGLPRLE
                     SVLKTFPELQFLGHSQAFWAEIGTNVIENGHRTPYPKGPVEPGRVVELMRTYPNLLGD
                     LSAGSGYNAISRDPEFGCAFLEEFQDRLFWGTDIANDPQDLPIVAWFEKIKTERLISE
                     DAFEKITWKNASRLLGLGLE"
     gene            5172..7244
                     /locus_tag="PKW60_03360"
     CDS             5172..7244
                     /locus_tag="PKW60_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPJ98498.1"
                     /translation="MALAAESEPLPLPAALTATSAALLVVALGLGIAYLRVRRHERAA
                     HEAVAERDCAIQAIRLGEERYRDLFNNINSGVAVLEVLGEGNDFILEDLNAAGERIAS
                     RFREDIVGRSLAEVDFTGVGAPLLDACRDVWRSGKSVRRYFSSRPAGRGERWFECFVY
                     KLSTGELVFVFEDITPQKRAEDENRLLATAIEYAGESIFITTPEGVVEYVNPAFERLS
                     GFSSVEAIGKTPRILKSGRHSTAFYAQLWAVLLRGETWRGRLTNKRKNGSLYEKEVTI
                     SPVLNPAGEVEHFISISRDLTQEMELAAQLRQAQKMEAIGTLAGGIAHDFNNILGAII
                     GFAELALEDMPEPTVTRKCIEEVRKAGRRAAELVHQILTFSRQTEQEHQPVQIQPVLK
                     EAMKLLRGTLPVTIAIQQEIDSECGQILADPTQLHQVIINLCTNAYHAMRERGGVLTV
                     TYRQLGIAPGEPERAPGLIPGDYAHLSVSDTGKGMNKATLERIFEPYFTTKPPGEGTG
                     MGLAIVHGIVRGHQGFVAVKSSPGEGSTFDVYIPLYRREEDATTPEGPTLIRRARGER
                     ILFVDDEESLALLARIALERSGYRVVSCTKSLEALRCFEETPQDFDVVITDQTMPDMT
                     GEELAERMRAIRPDIPIILCTGCSERMDERRAREAGFNGYLEKPFVDTDLSRAIQQAL
                     ADAQTKAP"
     gene            7346..8305
                     /locus_tag="PKW60_03365"
     CDS             7346..8305
                     /locus_tag="PKW60_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPJ98499.1"
                     /translation="MARPVRISTFATPALRFESSGRVFGQEAVDRMLAHWRAQLAQVL
                     PARPDLIVLPEACDRFPDHNNEERFEYYKIRGNQIRDALAGIAREHECYIAYSAAREV
                     EDGSWRNSTQLIDRAGNIAGIYNKNHLVIEERTRWGMLYGAEAPVFECDFGRVACAIC
                     FDLNFDELRLKYAAQKPELIVFSSMYHGGLMQAYWAYSCRAHFAGACCGLPSAILSPT
                     GEILATTTNYFSHVTATVNLDCVLIHLDHNWGRIRAMLDKHGPKVKMHDPGLLGAVLI
                     SSETPEFTIDNLIEEFEFELLDDYFQRSLDHRHASGNIEGTAG"
     gene            complement(8366..>9448)
                     /locus_tag="PKW60_03370"
     CDS             complement(8366..>9448)
                     /locus_tag="PKW60_03370"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPJ98500.1"
                     /translation="LLSRLSRDQLQGVIAHEMGHILNRDILFMQMLGVMAGAIVMISD
                     LYVRGLFYSSGGGRRRSRSSSRGKGGQAQALLLVLGLVFAVLSPIFAQLIYLAASRRR
                     EYLADAQSAVLTRYPEGLASALELIEQDSQQLVRLNRNIAPMFICNPYKRGLSAAGLM
                     RTHPPTAERIRILRTMAGAASFAEYQRASERIGAEVNVPASARAAEAGAPIRAAQAEA
                     PRAEKHEQRKLQWRQATDALRGVNKFMFLTCACGLRLKLPPDFKKEKVTCPRCRRELA
                     LPVAALAAARSLGDTLERATPPSQPEPRREQPKAQETLSVKRQPGAWTSFRCPCGQVT
                     NLPPGFQAPAVQCPQCKRSIRIDGAA"
BASE COUNT         1797 a         3085 c         2836 g         1730 t
ORIGIN      
        1 catgcgctga tgctgagcag tcgccccggt gatgcggcgc ggaatgatct tcccgcgttc
       61 tgtgacgtaa tgcttcagaa gattcacgtc tttgtaatcg atatatacaa cccggtcgac
      121 cgttaaccgg cagaccttgg cccggccggt accgcgcttc ttttttctgc gctttttcga
      181 ccgtttgccc attgctcgtg ccattgtgta ttcactcctg gttcagaaat cctagtgcta
      241 aaacgggacg tcgtcttcgg gaatcggctc ttcaatgacc cgcggcacgg gttttccgcc
      301 ggttccgcca tccgagtccc aggccagggt ctggatgcga tctgcctgca cttcgatttt
      361 gctgcgtttc tgtccggtgg ccttgtcttc ccactggtcg ctgcgaagca tcccctccac
      421 gaggatgggt cggccctttt tgaggttttc gccgcaatac tccgccgtgc cgcgccacgc
      481 cactacgtca acgaagaggg tctcttcgcg gcgttcgcct tcccgggttt tgtaattgcg
      541 gctgaccgcc aggcccatcc ggcataaagg ggttccgttg gacaggtaac gcaactcggg
      601 atcgcgcgtc aggcgacccg ccagaaaaac cttgttcagg tccggcatcc gcagatccga
      661 catccgtcca tccctccaag cgttgatgcg tcagtcgtca tcagatgccg ccgaccgcgc
      721 gggccgtcta cggcgtcccc gctcccggtc gtcgtcatcg tcatcatcgt cgtcatcgtc
      781 acgatagcgc gaggagcgcc gcctgcgccc gcggtcatca tcgtcatctt cctcgtcgtc
      841 caatgaccgt gccgccgcgc ccgcctgcag ctccagctgt ctccgctttt cctgctcggc
      901 ttcgagacgt aacgtgtgct cgtcgaaata gaccaccagg ctgcgaataa tcgcttccgt
      961 aagccggaaa taggtttcca gcttcgtaat gaacggcggc tccgcctgga accgcaacag
     1021 cacatagcag ccttcggtgc agcgcttgac ctcgtaggcc aatttgcgtt tgccctggat
     1081 ctccgagcgc acgatagctc cgccgttgtc agtgacaagc gtctcgacgt ttttggctat
     1141 cgtctggatc tcatcgtcct ttacttccgg gtggacgatg tagagcgctt cgtatgtccg
     1201 cactagtatt cacctccttt tttgtagatt ggtgccagaa gcatgaagtg catatcatag
     1261 cagacaaact ccggcgcctc aaatttcaca tcccgcgtct gtccgccacg acggccgcgg
     1321 tgatgcgccg agaccgccgg acacagggtg aatccggtgc ttcgaagcac gcccgtcaca
     1381 atacgttatg agattacgtg cgatcaagtc tatctatata ggcaagcaga cggatcagta
     1441 ccgcacccac gcgggctcgg ctatggccag ttcagcgcgc gtattgcgac gggaactcag
     1501 cccgatgagg ataattgaaa tttgcccggg atccaggcgg ccgttcgggt ccgggcgcga
     1561 ataagtggcc cagtaggcct ccgaaaataa cgccatcaac cgttgcggcc catcgccggc
     1621 ggacggcgcg cccagccggg caacataggt tgcgccggat tcctctatga attgcacatt
     1681 gacggtgcct tctccctcaa tcaatcccac ggtgaccgcg aggccgtcgt catcggcaga
     1741 cggccgttcg gacggttcca gccgcagccg cggatacgac cacggttccc catcgccaaa
     1801 ctgcatcgta aacacctgcg taccgccctc cgcaacctgg tgcgacatgg acgctcccgc
     1861 aacgatgttg tcttcccatc gggcggggtc tgcggcgcct tcgattgagt gcacgtgtcg
     1921 ggggacgaga ctgtcccgtt cgccgatcag ccggaatgcc agcacgggac gcccagcaga
     1981 cccgaaatca ccccggatat gcacccagtc gcccaccagt gcgggccggc cgttcgtggg
     2041 cagcgccaac tccagcgtca ccggcacacc atccaacggc ggaatttgaa tcagcccgcc
     2101 gggcagttcc acccgccaat caaccggcgc gctgtccacg gcgagacgac cttcgaccgg
     2161 ttcggcgctg aagttgtaca cccacagcgg catggccgac ggctcgcccg cggccagctc
     2221 gtaggcctgc gattccaagc gcacggcctc ttgcgggaac tccacctgca agaccactgg
     2281 cgagggggtc ccctcgcgca gcatcgcgag cggcggcggt ggctccagcg ccagctgtcc
     2341 gcatgttccc tccggaagca ccacaaagac ggcagccgga cccagttcca gcttcgtccc
     2401 gtccacccgg gcacggccca gataatcatg aaccgccacc ggggcgcggg cgaaaacggg
     2461 ctgccaggag aacgcagagt ccgcccaggc cacaagcacc tcgacgggat atccctccgg
     2521 ctgggcgtga aacgcgtagg cccaggcgtt tgcctcggcg tcgatgcaca ggcgcccgag
     2581 tggccgcgcg cccgcaagga ggcgaccgac ggccgccagc gccgcgtagc ccggccgcgg
     2641 ggtcagatca tcacgcagaa ggccaaactg gatgccccgt tccaggtaat tcccgagaat
     2701 gaaaaagaaa tggcgctcca cacccgaata caggctcgag gcatacgacg gcgcgacaaa
     2761 ccaggcctgg cgcaattcgt cctcgtccga catgtcttcc gcgggctcgg gttcggcggc
     2821 tttgaggcgt atgccgcatt ccgtcaacca gatgggtttt ccgctggcgg cgttccgcgg
     2881 cccctcgaac tcggtcagat aatcggcggg ggcgctgtag gagtgaatgt tgtaggtgtc
     2941 gaaatagggc caggcctcgt tcaacaggac accctcggta tgcaatgcgg agccgctgcc
     3001 tgcgtacaca ttccagcaga ccgtgagctc aggatcgccc gccttgaagg caagaaatgc
     3061 cgccttctgg aacgaacaca tctcgttgat cagatggccg ccaaaagacg tgatgttggc
     3121 ctcattccac ggttcccagg cctgcacgcg cccttggtat cgtttggcca gggcattgca
     3181 gaaccggtaa agcacccgga gatcccgcgg aaaccgcccg cccggactgg tgctgtcgag
     3241 ctcggtattt tgggcccact gcggtgtctg atgaaatacc tgcagcacgt tcaggccctc
     3301 ggccgccgcc gcggacgctg aggtatcgta cgaggtgctg tcttgagcga agacgcccgg
     3361 cgccgtctca agctcacccc acgacagacg atctcgcgac cagttcatgc ccgccagagc
     3421 cgaaagcgac gcaaacgcgc gctgttttgc cgcgtccttt cgcgcaaacc acgccatcgc
     3481 cgtatcaatg cagatcggtg aatccggcgg gatcggcacg atcagaggag ccagtactgc
     3541 cgcggaggtc cgggccaccg ccttcccgcc ggcgtccagg aactcaatcc ggtaccaccc
     3601 ggcaggaagc gcccccgtgg ccgcctgcgt ggcgccgttt acgcgcggac cgccgccctg
     3661 cgtctcaccg gcgtcatcga ggatacgaaa ctgggtccac gccgtgtccg gagggcccgg
     3721 tatctcgacc gcctcgcccg tgagaaagac atttcccgga tgtccgggca gcgggcctgg
     3781 gataccgcgt tccatccggt ctgctgcggt atccgaatcc ggctccgcac caatcgcatc
     3841 tgggctcgcc gccagaacgg ccgccagcag cagcagcgcg cctgcagaaa accaccatgt
     3901 ccggcatccg cggccatatc gcatcgtcag atccctcccg tgtcgcagat tcattccagt
     3961 cccaggccca gaaggcggct ggcgttcttc caggtgattt tttcaaatgc atcctctgag
     4021 atcagacgtt ccgtcttgat tttttcgaac caggcgacga tgggcaggtc ctgggggtca
     4081 ttggcgatat cggttcccca gaagaggcgg tcctgaaatt cttccagaaa cgcacagccg
     4141 aattcggggt cgcggctgat ggcgttgtat ccgctgcccg ccgacaaatc gcccaggagg
     4201 ttcggatacg tacgcatcag ttcgaccacg cggcctggtt ccaccggtcc tttcggataa
     4261 ggcgttcgat ggccattttc gatcacattg gtcccgattt ccgcccagaa tgcctgcgaa
     4321 tgccccagaa attgcagctc cggaaaggtt ttcagcacgc tttcgagacg gggcaggccg
     4381 gggtcgtcga agattccata gcatccgccg atctcggggc cgatatgaaa cgtcaggggc
     4441 agacttgcct tttcgaccgc ctcaaacaag ttcaggcagc ggggatcgtc aaagggaagg
     4501 ttgaacgtgc attcccccac acctttgcat cccaggtctt tgtaggcgct caaaaggtcc
     4561 gaaaaatccg cgctggcgtc attggtcagg taccggggat cgaggttgca gaacgggatc
     4621 aagcgttcgg gatattgggc gcagagctcg acgacgtcct cgaccgtcac cacggtatac
     4681 cggcattcgg ggctgacccc tcccatgata catgccttat cgatatgcgc ggcgtccatc
     4741 atctgaatca atctggctgg ctcgggataa tgcgagccat ttacgcgtgt gatcttcggg
     4801 tgccgccgcg gcgacgtgtg tgcatgaatg tcaatgaaca tcagatcctc ctcgcgcacc
     4861 tggtgccgtt cctgacaggc ggcgggcggc cttcccagcc gctattccaa tgggggcgcg
     4921 agccccccag ttatgttatc atgtctggcg cacgagcctc acaacggggc tcaagcgggg
     4981 gatgcccggt agccagagcc atgatgagga ccacaccgct ttctgccgag ggggaggccg
     5041 cggcccgtgt tcgggttcgg tcggtgtact ctccgaaggc ataacgggca cgaagggggt
     5101 atgttctgcg taaccggccg ttttactcga gtgtcgctgc cgcagccctg gcccatctcg
     5161 gcgcagcggg tgtggcgctc gccgccgaaa gcgagccgct gccgcttccc gcagccctga
     5221 cagctacttc ggccgccctg ctcgtggtgg ctctcggact cgggatcgcg tatctgcggg
     5281 tccggcggca cgaacgcgct gcccatgagg ccgtcgccga gcgcgactgc gcgattcagg
     5341 ccatccgtct gggcgaagaa cggtaccggg accttttcaa caacatcaac agcggcgtgg
     5401 cggtgttgga ggtgctcgga gagggcaacg acttcattct cgaagatctg aacgccgccg
     5461 gggagcgcat cgcgagccgg ttccgcgaag acatcgtcgg acgcagcctt gcggaggtgg
     5521 attttacggg tgtgggcgca ccgctgctcg atgcatgccg cgacgtctgg cgttccggca
     5581 agtccgtgcg gaggtacttt tcatccaggc cggccgggcg cggcgagcgc tggttcgaat
     5641 gttttgtgta caaactctcg acgggcgagc tggtatttgt ctttgaggac atcacgcccc
     5701 agaaacgcgc cgaagacgaa aaccggttgc tggctactgc gattgaatac gccggcgaga
     5761 gcatcttcat caccacgccc gaaggcgtcg tcgagtacgt caaccccgcc tttgagcgcc
     5821 tgtcaggatt ttcatccgtc gaagccattg gcaaaacgcc ccggatactc aaaagcggcc
     5881 gccacagcac agcgttctac gcgcaactgt gggcagtgct gctccgcggc gagacctggc
     5941 gcgggcgcct gaccaacaaa cgcaagaacg gctcgctcta cgagaaggaa gtcacgattt
     6001 cgccggtcct gaatcccgcg ggcgaggtgg agcatttcat atcgatctcg cgcgatctca
     6061 cgcaggagat ggaactcgcg gcgcagcttc gccaggccca gaaaatggag gccatcggca
     6121 cgctggctgg cggcatcgcc cacgacttca acaacatcct cggcgccatc atcgggtttg
     6181 ccgagctcgc cctggaggac atgcctgaac cgaccgtcac gcgcaagtgc attgaggaag
     6241 tgcgcaaagc aggccgccgc gccgcagaac tggttcatca aatcctcacc ttcagccggc
     6301 agaccgaaca agaacaccaa ccggtgcaga tccagcccgt tctgaaagaa gccatgaagc
     6361 tccttcgggg cactttgccc gtaacgattg cgattcagca ggagatcgat tccgaatgcg
     6421 gccagatcct ggccgatccg actcagttgc accaggtcat catcaatctc tgcaccaacg
     6481 cgtaccacgc catgcgggag cgcggcggcg tgctcaccgt cacctaccgc cagctcggca
     6541 tcgcgccagg cgaacccgag cgggcgcccg gcctgatccc cggagactac gcgcaccttt
     6601 ccgtgtccga cacgggcaaa ggcatgaaca aggccaccct cgaacgtatc ttcgaaccct
     6661 atttcaccac caagccgccc ggcgaaggca cgggtatggg actggccatc gtgcacggca
     6721 tcgtgcgcgg ccatcaggga tttgtggcag taaagagctc cccaggcgaa gggtcaacgt
     6781 tcgacgtcta tatcccgttg taccggcgcg aagaagacgc cactacaccc gaaggcccca
     6841 ccctcatccg gcgggcccgt ggcgagcgca tcctgtttgt ggatgacgag gagtccctgg
     6901 ccctgctggc gcgcattgcg ctcgagcgca gcggctaccg cgtggtgtcc tgcacgaaga
     6961 gcttggaggc tctccggtgc ttcgaggaaa cgccacagga cttcgatgtg gtcatcacgg
     7021 atcagaccat gccggatatg acgggagagg aactcgccga acgcatgcgc gcgatccgtc
     7081 ctgacattcc catcatcctg tgcacgggct gcagcgaacg gatggatgag cggcgggcgc
     7141 gcgaagccgg gttcaacggg tacctcgaga agccgtttgt tgacaccgac ctgagcagag
     7201 ccatccagca ggccctggcc gatgcccaga caaaggcgcc gtgacaccgg cgccggtctg
     7261 tccggagata ttccgcattc aaaaccgtta taatgccgcc aacacgaacc cctgaccggc
     7321 tgcccgcgcc aaaggaatgt ccgccatggc cagacccgtt cgtatcagca ccttcgccac
     7381 ccccgccctg cgcttcgaaa gcagcggacg agtcttcgga caggaggcgg ttgatcgcat
     7441 gctcgcccac tggcgggcgc aactggcgca ggtattgcct gcccggccgg atctcatcgt
     7501 cctgcccgag gcctgcgacc ggttcccgga ccacaacaac gaggagcgct tcgagtatta
     7561 caagatccgg ggaaaccaga tccgcgacgc cctcgccggc atcgcccggg aacacgaatg
     7621 ctacatcgcc tattcggcgg cacgcgaggt ggaagacggc agctggcgca actcgaccca
     7681 actgatcgac cgcgccggca acatcgcggg gatctacaac aagaaccacc tggtcatcga
     7741 ggaacgtacc cggtggggca tgctttacgg tgcggaggcg cccgtttttg aatgcgactt
     7801 cgggcgcgtg gcctgtgcga tctgtttcga cctgaatttc gatgagctgc gcctgaagta
     7861 cgccgcccaa aaacccgaac tcatcgtgtt ttcatcgatg tatcacggcg ggctgatgca
     7921 ggcgtactgg gcctattcct gccgtgcgca ttttgccggc gcctgttgcg gcctgcccag
     7981 cgccatcctc tcgcccacgg gggagattct ggccacaacc accaattatt tctcgcacgt
     8041 cacggccacc gtcaatcttg actgcgtgct tatccacctc gaccacaact ggggcaggat
     8101 ccgggccatg ctcgacaaac acggcccgaa ggtgaagatg cacgaccccg gactcctcgg
     8161 cgcagtcctg atcagcagcg aaaccccgga attcaccatc gacaacctga tcgaagaatt
     8221 cgaattcgag ttgctcgacg attattttca acgctccctc gatcaccgcc acgcgtccgg
     8281 gaatatcgaa ggcaccgccg gctgattcac caaacgcagc gccgggggga aacgcccgct
     8341 cgcagcccgc ttcgcgatcg acgccttacg cggcgccatc aatgcggatg ctgcgcttgc
     8401 actgggggca ttgtacggcc ggcgcctgaa aaccgggcgg gagattggtg acttggccgc
     8461 agggacagcg aaatgaggtc caggcgccag gttgccgctt tacagagagc gtctcctggg
     8521 ctttcggctg ttcgcgccgg ggctcgggct gcgaaggcgg tgtggcgcgc tccagcgtat
     8581 cgccaagcga acgcgcagcc gccagcgccg cgaccggaag ggccagttca cggcggcacc
     8641 gcgggcatgt gaccttctct tttttgaaat cgggcgggag tttcagccgg agtccgcagg
     8701 cacaggtgag aaacatgaac ttgttcacgc cgcgcaacgc atcggtcgcc tggcgccact
     8761 gcagcttccg ctgttcgtgt ttttccgcgc ggggtgcttc ggcctgggca gcccggatcg
     8821 gcgcacccgc ctccgcggcc cgggccgacg ccggcacgtt cacctccgcg ccgatgcgct
     8881 ccgacgcccg ctggtattcg gcgaacgagg cggcgcccgc catcgtgcgg agaatacgga
     8941 tgcgctccgc cgtcgggggg tgcgtccgca tgaggccggc cgccgagagc ccccgtttat
     9001 acggattgca gatgaacatg ggggcgatat tgcggttgag gcggacgagc tgctggctgt
     9061 cctgctcgat gagttccagg gcggatgcca gcccttccgg ataccgcgtg aggaccgcgc
     9121 tttgggcatc agcgagatat tcgcgccggc gtgatgccgc cagataaatg agctgcgcga
     9181 agatcggcga caacaccgcg aagaccaggc ccagcacgag caaaagcgcc tgcgcctgcc
     9241 cgcctttgcc tctcgaagac gagcgcgaac gccggcgtcc gcctccggac gaataaaaca
     9301 ggccccgaac gtacagatcg gagatcatca cgatcgcgcc tgccatgacg ccgagcatct
     9361 gcatgaacag gatgtcgcgg ttaaggatat gccccatctc gtgcgcaatc acgccctgca
     9421 gctggtcgcg gctcagccgc gagagcaa
//
LOCUS       DAOPBQ010000062         9341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_6985_length_9341_cov_4.631058, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000062 DAOPBQ010000000
VERSION     DAOPBQ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6985_length_9341_cov_4.631058"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2568
                     /locus_tag="PKW60_03375"
     CDS             <1..2568
                     /locus_tag="PKW60_03375"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPJ98501.1"
                     /translation="HVLREHGHPLPEKLEPHGSDARVHLQNAAGQYDLVILSLPETTT
                     LVLNRYSTAEFFSAVKRALNPDGVVATRVPGGANYLASESAFLGLSMMATLETAFENT
                     ALKPGESSWFFASGGGLMSAPMPLARRWQGIDGAESWYPAEGVRDFYRRGRVAQQFDA
                     YQAARASVPSDILVNTDLNPRALLFGILLELRRAGLRPAEIVPPLLLSGAYLMAAALL
                     AGAASRLVFLIRHGGSGNPAVLDTNVLIATTGLASMALTTVLLFFYQTRQGGLFLHIG
                     LIAALVMLGAWAGSLLVGRILARRRSESRWIVLGILAIHALALGLLAWMGPDASVWLF
                     LAAFFLCGMLTGAYFPAGALRLADAGQSPDASGARLEAFDHLGGAAGAALTGLLFLPF
                     FGTPRTLALLAGCLALNVPPLLLGRRARRVGSIAGDGFDRAVRPVAYTLTTLAALALA
                     ASNIFSAFGATQESAQLLDEVRLLAGPDAPLESVSATLSDGTAFTYFKETGPEGETAG
                     YLFATDALAGDVYGYGGPLVLAVSVDPGGIVRDYRILHSLETPVYLDFVQSWHEKLIG
                     KNIFEAQAFAQVDALSGATLTCNALMAALEQAGHRFAAEVLGKSVAPVSPGRRFTGEW
                     TGLAWLAGLSLAAIFARRRPNPWVRRAILVTALLACGWYFNVQYSTLQAASLAAGALP
                     PWTLSAPIFLVLALPLLTLLFGNFYCGYLCPFGAAQEVMEEVTRGVLVAETSKELWRY
                     ARAVKYLLLFMLALLYAVYRDGAVLQADPLITIFSGMPGRFVFYLGLGALTLSLVFRR
                     FWCRNLCPAGAFLSLCNGVRVFRRLRPPVQPAKCPLGVRDGTEFDCICCDRCRHESH"
     gene            2555..2815
                     /locus_tag="PKW60_03380"
     CDS             2555..2815
                     /locus_tag="PKW60_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98502.1"
                     /translation="MKATDALFLICVAVAAALFVVASLAHTGTAVPSALPAVSDGAGR
                     PRDLDINRVRKMIERGDLSDREAEFYREAPDAPQKESASPPG"
     gene            complement(2842..4971)
                     /locus_tag="PKW60_03385"
     CDS             complement(2842..4971)
                     /locus_tag="PKW60_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPJ98503.1"
                     /translation="MTRMMGMACLAAIFAVSAPASAAIWLVNANNVSGIEDGSTWDTA
                     FTDIQSAVDAASLAGGGQVWVAEGLYTSGASQVVAMAADVEIYGGFAGTETADQFDTR
                     NHLVHVTIIDGQGARRGVEGAADALIDGFLIRHGAPTTTIRGGGMQCLNVSPIISNCT
                     FQANTAEEGGGLALVKSAAQVRHCRFFANTASESGGALYASSSTGTISHSLFVSNTAD
                     HDGGAALCSGSTNLLFSDCTFASNKMVNGGSGDGGAVAMMGLSSSSFSGCRFIGNQAG
                     HGGALFSEDGHFTAVNSVFMHNIAGYSGAVHKWGDGNAVLTNCTVRRNWGDHSIYLGA
                     DLFRMTNCIVWDRLHYDVDPARIISLGYWGSKILTYNCIEGGQAGEGNIDVNPSFLPH
                     YSTIASELSYDPATFTSTATWGSATLQPGTLAGQVMELEGEDQWGDPMVRGYLILTND
                     TKSITVWGDATEGGAFTAPVSFTAFDWRVDNISSCIDSGRDTSGPAYGSVTTDIDGVA
                     RGQDGRGDGPTGPPPPGDRSDYDMGAAEVSAVPDLAVIDITFDPPAPQPGQEFTMLVT
                     VMNLGFNAAGPSVLSIEMTGDDAPLTYPVPSLGARESYVVERKATLAEGDGYSVTAIA
                     DCEGDVPADPNPANNTMTRLFNVHPPAWHVCDINRSGKVDAVDVQLVVNAALGLDIGG
                     LNADVNGENGVNAVDVQLVINAALGIL"
     gene            complement(5244..6200)
                     /locus_tag="PKW60_03390"
     CDS             complement(5244..6200)
                     /locus_tag="PKW60_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HPJ98504.1"
                     /translation="MRIVVLDGYTLNPGDNPWDDVAALGELTVYERTPAEQIIERAKD
                     AEIILTNKTPLSAQTLEQLPKLRFVSVLATGYNVVDVAAARTRNIPVSNVPVYGTNAV
                     AQFVFALTLELCHHVAEHSAGVKAGEWSRSPDFCYWNTPLIELAGKRMGIVGFGRIGR
                     RVGELAHAFGMEVLASDVQASNDPPYAPFSWCSIEDLFARADFVTLHCPQTADNARFV
                     NAALLQRMQRHAFFINTARGGLVNEADLADALHRGLIAGAAIDVVSSEPIAADNPLLT
                     APHCLITPHIAWAALAARQRLMRTTAENIAAFLAGAPVNVVN"
     gene            complement(6316..7743)
                     /locus_tag="PKW60_03395"
     CDS             complement(6316..7743)
                     /locus_tag="PKW60_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPJ98505.1"
                     /translation="MTETFCGVDFGTTNSSIALANSQHVRVLELDQANDNVTSLPSLL
                     YISREGERIVGRNAADAFIERNVNREVILRQVDLGINIEGYVASEPDKSEAYRPGAEP
                     DQRREAVRAQATVEVNSPGRLFQSLKSSLRFDGFRSTEVFGEHFQIEELTAMILAPMR
                     EAAEQAAGVSVQRAVFGRPVRFSHRPAEDRLAEERLRRAAHIAGFEEVVFFYEPVAAC
                     VEYAVATDRRQRLMVVDIGGGTCDVCIMEFGGASNEAQRLANSRILSVSGVPTAGDAV
                     DRDLIRERLYPCFGCKARYGPSQLPMPQYLFKNIADWQNLYKLNTEDIINWLIAAEAS
                     SDQPHCLRALRYLIQNNYGYPVRRIVEAAKKRLSFETEAMIDIHHGPIAISERVTRGE
                     FNAIIEETLEEMMGCVEEAESAAQLRPDQIDLVLTTGGTSLTPAVRHMLETRYGADRI
                     RRRDTFSSVAMGLAIVARYSSGSIS"
     gene            complement(8097..8387)
                     /locus_tag="PKW60_03400"
     CDS             complement(8097..8387)
                     /locus_tag="PKW60_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98506.1"
                     /translation="MARHAIKPDKRFHYRYVAAELMWEASRLLPNNDPMTARALYDAG
                     RWLMDRDPEAADKYYKALVRRCRKLPVAQQADERRWFPRDVTDADFAVPARN"
     gene            8591..8818
                     /locus_tag="PKW60_03405"
     CDS             8591..8818
                     /locus_tag="PKW60_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPJ98507.1"
                     /translation="MIKKLVRHGNSLALVIDKAILELLRIDHDTPLELITDGDVIVIK
                     PERDEDHEKKLHLVLEAVNEKYGRALKRLAE"
     gene            8831..9223
                     /locus_tag="PKW60_03410"
     CDS             8831..9223
                     /locus_tag="PKW60_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HPJ98508.1"
                     /translation="MDRPEFLSLADALTIHEDQIGRYGGSHGIRDQGLLESAMAQPLA
                     TYGGEYLHNGLYEMAAAYLYHIVQNHPFIDGNKRVGAVSAYVFLRMNGIELICSEEAF
                     ESLVLAVASGQAGKAQAAAFFEQHSRPV"
BASE COUNT         1666 a         2928 c         2845 g         1902 t
ORIGIN      
        1 cacgtgcttc gcgaacatgg gcaccctctg ccggaaaagc tcgaaccgca cggcagtgac
       61 gcgcgcgtcc atcttcagaa cgcggccggt cagtacgatc tggtgatcct gagcctgccc
      121 gagaccacga cgctggtgct caaccgctat tcgaccgccg agttcttttc cgcggtgaaa
      181 cgcgcgctga atcccgatgg cgtggtggct acgcgagtgc cgggcggcgc caactacctc
      241 gccagtgaat ccgcctttct cggattgtcg atgatggcaa ccctcgaaac cgccttcgag
      301 aacaccgcgt tgaaaccggg cgagtcttcc tggttctttg cgtccggcgg cggcctcatg
      361 agcgctccga tgcctctggc tcgccgctgg cagggcatcg acggcgcgga gagctggtat
      421 cccgctgaag gcgtgcgcga tttttaccgg agagggcgcg ttgcgcagca gtttgacgcc
      481 taccaggccg cccgcgcctc cgtaccttcc gacatactgg tcaacacgga tctcaatccc
      541 cgcgcgctgc ttttcggcat cctcctggaa ctccggcgtg cgggattgcg ccccgcggag
      601 atcgtgccgc cgctgctctt gagcggggcg tatctgatgg ccgccgcgct gctggcaggc
      661 gctgcgagcc ggcttgtgtt tctgatcagg cacggcggtt cgggcaaccc cgccgttctg
      721 gacacaaacg tgctgatcgc cacgacgggc ctggccagta tggccctgac taccgtgctc
      781 ctgtttttct accagacgcg gcagggaggc ctgtttctgc acatcggcct cattgccgcg
      841 ctggtgatgc ttggcgcgtg ggccggcagc ctgctcgtgg gccggattct ggcgcgccgc
      901 cgcagcgaat cccgctggat cgtgctcgga attctcgcga tccatgccct ggcgctcggc
      961 ctgctcgcct ggatggggcc ggatgcgtcc gtgtggctgt ttctcgccgc attttttctc
     1021 tgcgggatgc ttacgggcgc ctatttcccg gcaggagcgc tgcgcctggc ggacgccggc
     1081 cagtcaccgg atgcgtccgg tgcgcggctt gaagccttcg accatcttgg cggtgcggcg
     1141 ggcgccgcgc tgacgggact gcttttcctg cctttttttg gcacgccgcg caccctggcc
     1201 ctgcttgcgg gctgtctcgc gctgaacgtt cccccgctgc tgctgggccg ccgcgcacgc
     1261 cgggtgggct ccattgcggg agatggattc gatcgggcgg tccggccagt cgcgtatacg
     1321 ttgacgactc tggcggctct ggcgctcgcc gcatccaaca ttttctcggc attcggggcg
     1381 acacaggaat cggcgcaact gcttgacgag gttcgtttgc tcgccggacc ggacgccccg
     1441 ctcgagtccg tatccgctac gctttcggat ggcacagcct tcacgtattt caaggaaacc
     1501 gggcccgagg gcgagacggc agggtatttg ttcgccacgg acgcgctcgc gggcgatgtt
     1561 tatggatatg gcggcccgct ggtgctggcc gtttccgtcg atccgggggg catcgtacgc
     1621 gattaccgca tcctgcactc cctggaaacg ccggtgtatc tcgattttgt gcagtcctgg
     1681 catgagaaat tgatcggcaa gaatatcttt gaggcgcaag ctttcgcgca ggttgacgcg
     1741 ctgagcgggg caacgctgac ctgcaacgcg ttgatggccg cgctcgagca ggccggccac
     1801 cggtttgcgg ccgaggtact cggcaaatcg gtggccccgg taagcccggg caggcgcttc
     1861 acgggcgaat ggaccggcct ggcttggctc gctggattat ccctggccgc cattttcgcg
     1921 cgccgccggc ccaatccctg ggtgcgccgg gccatccttg tgaccgcgct gctcgcctgc
     1981 ggctggtatt tcaacgtgca gtactccacc ctgcaggcgg ccagcctggc cgcgggcgcc
     2041 ctgcctccct ggaccctgtc cgcgccgatc tttctggtgc tggcgctccc gctgcttacg
     2101 cttttgttcg gcaatttcta ttgcggctat ctgtgcccgt tcggggcagc gcaagaggtc
     2161 atggaagagg tgacccgggg cgttcttgtc gccgagacgt ccaaagagct ctggcgctac
     2221 gcgcgcgcgg tcaaatatct tctgcttttc atgctcgctc tgctctatgc cgtgtatcgc
     2281 gacggcgcgg tgctgcaggc cgacccgttg atcaccatct tcagcggcat gccgggccga
     2341 ttcgtctttt accttggcct cggagcattg acgctttctc tcgtatttcg ccggttctgg
     2401 tgccggaatc tgtgtccagc cggggcattc ctgtcgctgt gtaacggcgt tcgtgtgttc
     2461 cggcggcttc ggcctccggt tcagcccgcg aaatgccccc tgggcgtgcg cgacgggacc
     2521 gaattcgact gcatttgctg cgacaggtgc cgtcatgaaa gccactgacg ccctgtttct
     2581 gatttgcgtg gccgtggccg cggcgctgtt cgtggtcgcg agcctggcgc acaccggtac
     2641 cgccgttccg tctgccctgc ccgcggtatc cgatggagcc ggccgccccc gcgatctcga
     2701 catcaatcga gtccggaaaa tgatcgaacg cggtgatctg tccgatcggg aagctgagtt
     2761 ttaccgcgag gcacctgacg caccgcaaaa agaatccgcc tccccaccgg gatgaggagg
     2821 cggcgtatgt tcgggttcgg gctacaggat gccgagcgcg gcgttgatca ccagttgcac
     2881 atcaacggca ttcacgccat tttctccatt cacgtcggca ttgagaccgc cgatgtccag
     2941 gccgagcgct gcgttgacta ccagctgcac gtcgaccgcg tccacttttc cgctgcgatt
     3001 gatgtcgcat acgtgccagg cgggcggatg cacattaaac aggcgcgtca tcgtgttatt
     3061 ggctggattc gggtcggcgg ggacatcgcc ttcgcagtcg gcaatggcgg taacgctgta
     3121 cccgtcgcct tcagccaggg tggccttccg ctctaccaca tagctttccc gcgcgcccag
     3181 gctcggcacg ggatacgtca gcggtgcatc gtcacccgtc atttcgatgg aaagcacgga
     3241 gggcccggca gcgttgaagc caagattcat gaccgttact aacatcgtga actcctggcc
     3301 cggctgaggc gccggcggat cgaacgttat gtcaatgacc gccaggtcag gcaccgccga
     3361 gacctctgcg gcgcccatat cgtaatccga gcggtctccc ggcggcggcg gaccggtcgg
     3421 gccgtcgcca cggccatcct gcccccgggc gacgccgtcg atgtcggtcg tgacggatcc
     3481 gtaggcgggg ccactggtgt cgcggccgct gtcaatacag gacgatatgt tgtccacgcg
     3541 ccaatcaaac gctgtgaatg agactggcgc agtgaatgcc ccgccctcgg tcgcatctcc
     3601 ccagacggta atcgacttgg tatcgtttgt caggatcaga tagcccctga ccattggatc
     3661 accccattgg tcctctccct cgagctccat tacttgtccg gccagggtac ccggctgaag
     3721 cgtggccgat ccccaagtcg ctgtcgaggt aaaagtggcc gggtcgtacg aaagctcact
     3781 ggctatggtg gagtagtgcg ggagaaatga gggatttacg tcgatgtttc cctcgccagc
     3841 ctggccgcct tcaatgcagt tgtatgtcaa gatttttgag ccccaatacc ccagagagat
     3901 gatcctggca gggtccacgt cgtaatgcag cctatcccag acaatgcagt tcgtcatccg
     3961 gaaaaggtcg gcgccgaggt agatgctgtg atcgccccag tttcggcgca cggtgcagtt
     4021 tgtcaagacc gcgttgccgt cgccccattt gtgcacagcg ccgctatagc ctgcaatatt
     4081 gtgcatgaac acactattca cggcagtgaa gtgtccgtct tcggaaaaga gtgcaccgcc
     4141 atgcccagcc tgattgccaa tgaaccggca cccggaaaag gaacttgatg acaatcccat
     4201 catcgcaacg gccccgccat cgccagagcc gccattgacc attttgttgc tggcaaacgt
     4261 gcagtcgctg aacagcagat tcgtgctgcc ggaacacaat gcggccccgc catcatgatc
     4321 ggccgtattg ctcacgaaaa gggagtgcga tatcgtgccg gtgctgctcg atgcgtacaa
     4381 ggctcccccc gattccgatg cggtattcgc gaaaaaccgg cagtggcgga cctgcgcggc
     4441 agactttacc aaggccaggc cgccgccctc ctccgctgta ttcgcctgaa acgtgcaatt
     4501 tgagatgatg gggctgacat tcaggcactg cattcccccg cctctgatcg tcgttgtggg
     4561 tgcgccatgt ctgatcaaaa aaccatcgat gagggcgtca gctgcacctt cgactccgcg
     4621 acgcgccccc tggccgtcga tgatcgtcac atggaccagg tggttgcgcg tgtcaaactg
     4681 atctgcggtc tccgtaccgg cgaatccgcc atagatctcg acatcggcgg ccatggccac
     4741 cacctggctg gccccgctgg tatacaggcc ttcggccacc cacacctggc cgccgcccgc
     4801 cagactcgcc gcgtccaccg cgctctggat gtccgtgaat gccgtgtccc aggtgcttcc
     4861 atcttcgata cccgatacat tgttcgcatt gaccagccaa atggcggccg atgcaggtgc
     4921 agataccgcg aatattgctg ccaggcacgc catccccatc attctcgtca tcgcataccc
     4981 ctttcgttga gccgcagtct tcgcatgcca gctcccggtg ctcaccgagc gcttcccaaa
     5041 tgctaacaga gactaccaca ctacctgtcc aaaacgcatt gagttctggg cacatttcag
     5101 tgtaaacggg agtggaactg acgggtttgc gccttaaatc cccggtacgt catgaattaa
     5161 cggaaatcct gccttcgaaa acagctgcgc cccgtaaaaa tcatggtact ggccctgccg
     5221 gcgtctgtat cttgcgcaga agctcagttg acgacattaa cgggtgcgcc ggcgagaaat
     5281 gcggcgatat tctcggccgt agtgcgcatg agccgttgcc gtgccgccag ggctgcccag
     5341 gcgatgtgcg gagtgatgag gcagtgcggc gcggtcagca acgggttgtc cgcggcgatg
     5401 ggctccgagg agaccacgtc gatggcggcc ccggcgatga ggccgcgatg caatgcatcg
     5461 gccaaatccg cttcattgac cagcccgcca cgggccgtat tgatgaagaa ggcgtgccgc
     5521 tgcatgcgtt gaagcaacgc cgcgttgaca aatcgggcgt tatcggcggt ctgggggcag
     5581 tggagagtga cgaaatcggc cctggcaaac aggtcttcga tgctgcacca tgaaaacggg
     5641 gcgtagggcg ggtcgttgct ggcttgcacg tcactggcca gcacctccat gccgaatgcg
     5701 tgcgcgagct cgcccacccg ccggccgatg cgcccgaacc ccacaatgcc catgcgtttg
     5761 cctgccagtt cgatgagcgg cgtattccaa tagcagaaat cggggctgcg gctccattcc
     5821 cctgccttga cgccggcgct gtgttcggcc acgtgatggc acagctcgag cgtcagcgca
     5881 aaaacgaatt gcgccacggc attggtgccg taaaccggca cattggacac ggggatattc
     5941 cgtgtgcgcg cggcggccac gtctacgacg ttgtagcccg tggcaagtac ggagacaaag
     6001 cggagcttcg ggagttgttc cagggtctgg gccgaaagcg gcgtcttgtt ggtaagaatg
     6061 atctccgcat ctttggcgcg ctcgatgatc tgctcagcag gcgtgcgctc gtaaacggtc
     6121 aactcgccca gggcggctac gtcgtcccag gggttgtcgc caggattcaa ggtatatcca
     6181 tcgagcacta caatccgcat gatattgtct ctcctgtaca tccgcgcgac tctcgccgct
     6241 gattaaggtc aaccggcgcg gccagagcaa gttccggcgc ttcaggcctt catcgcttcc
     6301 ggggcatcgg gcacttcatg agatgctgcc tgaggaatac cgcgcgacga tggccaggcc
     6361 cattgccacg ctcgaaaacg tgtcacgccg ccggatgcgg tccgcgccat agcgcgtttc
     6421 gagcatgtgc cgaacggcag gagtgaggct cgtgccgccg gtggtcagga caagatcgat
     6481 ctgatccggg cgcaattggg ccgcgctttc ggcttcttcg acacagccca tcatttcctc
     6541 gagcgtttcc tcgatgatcg cgttgaattc gccacgggtg acccgctcag aaatggcgat
     6601 cggcccatga tgaatgtcga tcatcgcttc ggtctcgaaa ctgagccgct ttttggcggc
     6661 ctcgacgatc cgccgcacgg gatacccgta gttgttctgg atgagatacc gaagtgcgcg
     6721 caggcaatgg ggctgatcgg aactggcctc ggcggcaatg agccagttga tgatgtcctc
     6781 ggtattgagc ttgtagagat tctgccagtc ggcgatgttc ttgaacaggt actggggcat
     6841 gggcaactgt gaggggccgt agcgggcctt acacccgaaa cacgggtaga gcctctcacg
     6901 gatgaggtcg cgatcgactg catcgccggc ggtggggacg cccgatacgc tgaggatgcg
     6961 gctgttggcg agccgctggg cttcgttcga tgcgccgccg aattccatga tgcacacgtc
     7021 gcaggtgccg ccgccgatat ccaccaccat caggcgctgg cgccggtcgg tggccacggc
     7081 gtattccacg caggccgcta ccggctcgta gaagaacacc acttcctcaa agcccgcgat
     7141 gtgcgccgcc cgtcgaaggc gctcttctgc gaggcggtcc tcggcgggcc ggtgggaaaa
     7201 gcgcaccggg cggccgaaga cggcacgctg aactgagacg ccggcggcct gctcagcggc
     7261 ttcccgcatg ggtgcgagga tcatggccgt cagctcctcg atctggaaat gctcaccgaa
     7321 gacctcagtg ctgcgaaacc cgtcaaagcg caaggagctt ttcaacgact gaaacagccg
     7381 tccgggcgaa ttgacctcga ccgtggcctg ggcgcgcacg gcctcacgcc gctggtcggg
     7441 ttcggcgccg gggcggtagg cctccgactt gtccggctcg gatgccacat agccctcgat
     7501 gttgatcccc agatcgacct gcctgaggat cacctcccgg ttgacattcc gttcgatgaa
     7561 ggcatcggcc gcatttcgcc cgacgatccg ctcgccttcc cgcgagatgt acaacagcga
     7621 aggcagcgaa gtcacgttgt cgttggcttg atcgagttca agcacccgca catgctggct
     7681 attggccagc gctatactcg aattcgtcgt gccaaagtcc acgccgcaaa aggtctcggt
     7741 cataccattc cccgtgctcc ggcgatcgcc cgcaccgttc catccggcgc agtatttttc
     7801 cgataacacc acgcgccgcg agggcatttc cgcccccgcg acgcgtgttc tgcactggtt
     7861 gtcaggtaat tgcgaattct gccatatccg atgcaccgga atccagcgcg cattccgctg
     7921 ctggctacac ggtcttcggg cttagccttt tcccgcaggg gccgcccggc gctcaaccgg
     7981 agaaggcggg gccaggaagg gcgcacccgc actccaatgg tcaacgcata gtgagaatgt
     8041 gaaatacgcg cggtttgtaa acgcacactg gggcgatgcg tacgcagata gtcgcgctag
     8101 ttgcgcgcgg ggactgcaaa atctgcgtca gtgacatcgc gcggaaacca gcgccgctcg
     8161 tctgcctgtt gagcgacagg cagctttcgg caacggcgaa ccagggcctt gtaatatttg
     8221 tccgcggcct cgggatcacg gtccatgagc caccggcccg cgtcatacaa ggcccgtgcg
     8281 gtcatcgggt cgttgttggg caacagcctc gaagcttccc acatcaactc tgccgcgaca
     8341 tagcggtagt gaaaccgttt atccggtttg atcgcatgcc gcgccacccg ggcttgttcg
     8401 ctggctttcg gaagaacctg gttccaaaac ttttcgggac agccatacgg cgcgttgcgg
     8461 ttgccctgtt ttgacggaca cgccggggtg gactcttagc tcccttgctc gcttccgccc
     8521 acgggctcat tgctcttcgc ccttccgggt gatacaatgt agatacgaaa gatcgacaag
     8581 gagccctgtc atgatcaaga aactcgtacg ccacggaaac agcctggcgc tcgtcatcga
     8641 caaagccatc ctcgaactcc tccgcatcga ccacgacacc cccctcgaac tcatcaccga
     8701 cggcgacgtc atcgtcatca agccggagag ggatgaagac catgagaaga aattgcatct
     8761 ggtccttgag gccgtcaatg aaaaatacgg cagggcgctt aaacgacttg cggagtagtg
     8821 acggatgcca atggacaggc cggagtttct cagcctcgct gatgccctga cgattcatga
     8881 agaccagata ggccggtatg gcggatcgca tgggatacgc gatcaagggc ttctggaaag
     8941 cgctatggca caacctcttg ccacgtatgg cggggagtat ttgcacaatg ggctctatga
     9001 aatggcggca gcctacctct accacattgt gcagaaccac cccttcatcg acggcaataa
     9061 acgtgtgggg gctgtttcag cctatgtctt tctgcggatg aatggcattg aactcatttg
     9121 ctccgaggaa gcgtttgagt cgctcgttct cgcggtcgct tcagggcagg ccggcaaggc
     9181 acaggcggcg gcttttttcg agcaacacag ccgcccggta tgaggacggc gccggttttc
     9241 caaaagaacg aattcgcgca aaaaagcgcg gcaaaccacg gccaaaacaa agagcccgcg
     9301 acgaatcgcg ggctctggtt tgtttgtatg tactcagcgt g
//
LOCUS       DAOPBQ010000063         9205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7098_length_9205_cov_4.500765, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000063 DAOPBQ010000000
VERSION     DAOPBQ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7098_length_9205_cov_4.500765"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..2306
                     /gene="pnp"
                     /locus_tag="PKW60_03415"
     CDS             33..2306
                     /gene="pnp"
                     /locus_tag="PKW60_03415"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPJ98509.1"
                     /translation="MIERLSVNIGGKDLCFETGRIARQAHGSVLVTLGDTMVLCAVCV
                     SPEARPGQDFFPLTVDYREKSSAAGKIPGNFFRREARPSEREVLVCRLTDRPIRPLFP
                     KGFTNEVQICSTVYSTDNEHNPDVLAINAASAALHISKLPFDGPIGAVRIGMIDDELV
                     VNPLMTSEMELSALDLVIAGTSEAILMVEGLADEVPEETLLEALDLGHQEIKKICAAI
                     EEFRARAGVEKMPFEAPSLDPQVISDVERITRDSLKDALAVTGKHERQDAVDAVETRL
                     EEQLLSEYGEEKFQEVAGDIREAFGDLERALMRQAVIQTNTRVDGRDLTTVRDITIEV
                     GVLPRAHGSCLFTRGETQALVTTTLGTRSDEQRLDELTGEEFRRFMLHYNFPPWSVGE
                     VRRIMGPGRREIGHGKLAERALSGILPFMSEEDPENASPEDDFPYTIRVLSDITESNG
                     SSSMATVCGGTLSLMDAGVPIIAPVAGVAMGLIKEGDEARVLTDILGVEDHLGDMDFK
                     VCGTAQGITAFQMDVKIKGISRELMSKALDQAREARLHVLDKMLETLPEPRPDVSPYA
                     PRIYTIKIDVDKIRDVIGPGGKVVRGIQMRTGAEINIEDDGTIHVASVDKDSADEALA
                     MIRSITAEPEVGAIYPGTVSRVLNFGAFVSFMGGKEGLVHISELAPGRVNRVEDVVNI
                     GDEISVKVVEIDHMGRLNLSKVQADAELGHLSEEDLAEMERSRGRERNHGDRDRGGRD
                     RGGRDRDRGGSRGRPRR"
     gene            2363..3646
                     /locus_tag="PKW60_03420"
     CDS             2363..3646
                     /locus_tag="PKW60_03420"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPJ98510.1"
                     /translation="MARAFSFAAETSPQVRQLANGITLLTERLPHVRSVSLGVWITTG
                     SANEPPHLNGISHFLEHLLFKGTTTRTARQLMEAIEAHGGHLNAYTAREYTCVYAKTL
                     DSYLDTAVEILGDLVCDSQLYDFEKERNVILEEIASNDDIPEELIHDLFMSNIFPRHS
                     LGAPISGELKSVEQLSQDDVRAYYATWYRPGNMVISAAGHFDDDEILDAIGKAFVSLP
                     VGAAMAVPAYEAPTLGAGIREYRRKIAQSHYCIGFPGPATDSPDRFVYDVLCNALGGG
                     STSRLFERIREQEGLSYAIYSFQSAYYRAGLLGIYAAVAPENLNRALSVTFEELRGVR
                     DNGLPAVEIEQNREQLKGSLLMALEDTFHRMSRMAKSHIYYGRVVPVEEVIAQVDAVT
                     AEAVQELADRILRASKCMVTVLGPKNRLHLPEIPL"
     gene            3643..4098
                     /gene="dut"
                     /locus_tag="PKW60_03425"
     CDS             3643..4098
                     /gene="dut"
                     /locus_tag="PKW60_03425"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPJ98511.1"
                     /translation="MNPPLRVQIIREPGTEDIALPQYETAHAAGMDLRAAVPEPVLLR
                     PGERALVPAGLRIALPPGYEAQIRPRSGLAIRHGLTLLNSPGTIDADYRGEICIIIAN
                     LGQEPFTIERGDRIAQMIIAPVTRVEWDVVNALDTTSRGDGGFGSTGRQ"
     gene            4152..5222
                     /locus_tag="PKW60_03430"
     CDS             4152..5222
                     /locus_tag="PKW60_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98512.1"
                     /translation="MHTFTVIAGVLAASAPAQPAPPADVLVGVNYFAGWWEPLPNKWH
                     DATGEDWRPRYPERVPTLGEYNTQETMDREIIAAASHGVDFFAILWYYNPPGTEAEPN
                     ARFLERGVTNFIASPENHRMRFMLEFCNHPPFEVATDEDWTRCVELWTSWIQHPQYLR
                     IDGKPVFKVHGFHHFYTQNGRDPAFCKKRLDQLRQSVRDAGAGEMLIGCGVGEHDVIA
                     EGHPAVSLFDFTAVYMGVPPLEQRSEDYPYKTLLDRAQVSWKTHGGDAIPHMPYLPAG
                     WSPRPWHDPRPSFAFPTRGEWDGALSDIKAALTTTSILGLPGQKAFTIYAWNEFGEGG
                     IVAPTQGEGTMKLEGIQANFHP"
     gene            5351..6460
                     /locus_tag="PKW60_03435"
     CDS             5351..6460
                     /locus_tag="PKW60_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98513.1"
                     /translation="MLFSAVCVASVFVLTAAEPEALSDAPFPDPLLLENGTRVNTRED
                     WEARRTEMRDLLLRHEYGSIPTAPVPIAHAVILTEELQNDDVTLFRDVRLAVGSENPF
                     PVRVEVYLPAKGPGPFPTILCVGLKYPEVQVINGRGYAFVCYENEDFDFDTQGGDKVG
                     PAQAMFPDCDWGSLGAWAWGASRVLDYLLTLPEVDAQHVVVTGHSRRGKTALLAGALD
                     ERFAIVVPNGSGCGGAAAYRRYGPECETLEAITLPSRFESWFQKDFSRFAGKEAELPF
                     DQHFLRALVAPRPVLSTDAFGDLWANPIGTAAAWRAAQPVFDFLEVPENNAMHFREGK
                     HAHLPEDYEALLDFSDHHFFGKPLTCTYNEGPEEK"
     gene            complement(6561..>9205)
                     /locus_tag="PKW60_03440"
     CDS             complement(6561..>9205)
                     /locus_tag="PKW60_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98514.1"
                     /translation="NLFLQRKDPAFFDVAVKPFIANKLDKTFLDHYLLGDELAAYLAP
                     WRYSQLNTLERILLAERVAAERENTLRFITDQYNLIPPDVDRFNHLFMTALRGHSLDV
                     NGLARGYGGGALFASRQGARMDMGGDWGAFEVTSTSAEPAAEDAVAMQLDVAEEAAPA
                     EAAKPEPGRVFVKDEKRSDRYRALYTAMEKTREWVENNYFELPVEQQTADRVSVNAFW
                     RDYAGRDASAPFLSGNLAEASHNFTEVMFALAVLDLPFEAGEHQVERSAARITLTAGG
                     PAVVFHKEAAPADMPEDTPPILVSQNFFRSDDRFRLVDNERVDKFVVDEFITQVVYGC
                     QTVVTNSTSSRQRLDVLLQIPEGAVPVERTQYLKTYSLALEPYHTATIETYFYFPEEG
                     LFEHFPVHVAKDGKLAAFAQSVAMKVVAALSTVDTASWPYVSQHGTDEEVYQYLRNEN
                     IHAASDRTINLDLIAWRMKDPAVFANTLDILRQRRAFAPTLWSYALLHNDTDAGREYL
                     QFRDDFAGQCGAAIDSPLLRLDPVLRKTYQHMEYSPLVNARAHRLGPRRVIVNARLAS
                     QYTRLLEILAHRPALDQHDLMSVAYYLMLQDRIEEGLAFLGKVRPDAIDMRLQYDYLA
                     CCAAFYTEEPEKAAAIAGTYASYPVDRWRNRFANVAAQVREIQSATTTVIDAKSREQV
                     QEQLAQTEPDFDFKVEARQVTIAYQNLTACRVAYYLMDIELLFSRDPFARQGAGHFST
                     VRPNFTEERELPASQTTLTFDLPSQFSSSNVLIEIAAGGKTKTQTCYAANLAVQVIEN
                     YGQLRVTSLETGQALSKVYVKVYARLQGGEIRFYKDGYTDLRGRFDYSSLNTDELDQV
                     ERFALLILSDTDGAAVREAAPPKR"
BASE COUNT         1780 a         2767 c         2900 g         1758 t
ORIGIN      
        1 agacgttccc agacccaacg gagtgcagca gtatgattga acgtctctct gtaaacatcg
       61 gcggcaagga tctctgtttt gagaccggcc gtatcgcgag acaggcgcac ggctcagtgc
      121 tggtcaccct gggcgatacg atggtgctct gcgccgtatg cgtctcgccg gaagcccgcc
      181 ccggccagga tttcttcccc ctgacggtcg actaccgcga gaagagttct gccgcgggaa
      241 agatccccgg caatttcttc cgccgcgaag cacggcccag cgagcgcgaa gtattggtgt
      301 gccggctcac ggaccggccc atccgcccgc tctttcccaa gggcttcacc aacgaggtgc
      361 agatctgctc gaccgtctac tcgaccgaca acgagcataa tcccgatgtg ctggcaatta
      421 acgccgcgtc ggccgcgctg cacatctcga aattgccgtt tgacgggccc atcggcgccg
      481 ttcgcatcgg catgatcgac gacgaactcg tggtgaatcc gctcatgacg agcgaaatgg
      541 agctgagcgc actcgacctc gtcatcgccg gcacgagcga agccatcctc atggtggagg
      601 gccttgcgga cgaagtgccg gaagaaacgc tcctggaagc ccttgatctg ggccaccagg
      661 aaatcaagaa gatctgcgcg gccattgagg aattccgcgc ccgcgccggc gtcgagaaaa
      721 tgccgttcga ggcgccatcc cttgacccgc aggtcatcag cgatgtcgag cgcatcaccc
      781 gggattccct gaaagacgcg ctcgcggtca ccggtaaaca cgaacgccag gacgcggtgg
      841 atgccgtcga aacccggttg gaagagcagc tcctgtccga gtacggcgag gagaagtttc
      901 aggaagtcgc gggcgatatc cgcgaagcgt tcggcgatct ggaacgggcc ctgatgcgcc
      961 aggcggtcat tcagaccaat acgcgggtcg atggccgcga cctgaccacg gtgcgcgaca
     1021 tcacgatcga ggtgggcgtg cttccgcggg cccacggttc gtgcctgttt acgcggggcg
     1081 agacgcaggc cctggttacg accacgctcg ggacgcgcag cgatgagcag cggcttgatg
     1141 aattgaccgg cgaggaattt cgccggttca tgctgcacta caacttcccg ccgtggtcgg
     1201 tgggcgaagt gcgccgcatc atgggtccgg gccggcgcga gatcggccac ggcaaactgg
     1261 ctgaacgcgc cctctcgggc atcctgccct tcatgtcgga ggaagacccc gaaaacgcgt
     1321 cgccggaaga cgatttcccg tataccatcc gcgtgctgtc ggacatcacc gagagcaacg
     1381 gttcgagttc catggcgacg gtctgcggcg gcacgttgag cctgatggac gccggcgtgc
     1441 ctatcattgc gccggttgcc ggcgtggcca tgggcctcat caaagaaggc gacgaagcac
     1501 gcgtgctgac cgatatcctt ggcgtcgagg accatttggg cgacatggat ttcaaggtct
     1561 gcggcacggc gcagggcatt acggcgttcc agatggacgt caagatcaag ggcatcagcc
     1621 gtgagttgat gagtaaggcc ctggatcagg cgcgcgaagc tcgccttcac gtgctcgaca
     1681 agatgctgga aaccctgccc gaaccgcgcc cggatgtgtc tccctacgca ccgcgcatct
     1741 acaccatcaa gatcgacgtc gataagatcc gcgatgtgat tggaccgggc ggcaaagtgg
     1801 tgcgcggcat ccagatgcgc accggcgcgg aaatcaacat cgaagacgac ggcaccattc
     1861 acgtggcgtc ggtggataag gacagcgcgg atgaagcgct cgcgatgatc cgcagcatta
     1921 cggcggaacc cgaggtgggc gcgatctacc cgggcacggt gagccgggtg ctcaatttcg
     1981 gcgcgttcgt cagcttcatg ggcggcaaag aagggctcgt gcacatctcg gaactggcgc
     2041 cgggccgcgt gaaccgcgtc gaggacgtcg tcaatatcgg cgatgaaatc agcgtcaagg
     2101 tggtcgagat cgaccacatg ggccgcctca acctgtccaa agtgcaggcc gatgccgagc
     2161 tgggccacct cagcgaggaa gacctggctg aaatggaacg ctcgcgcggc cgggagcgga
     2221 atcacggaga ccgcgaccgc ggcgggcgtg atcgaggcgg acgggaccgc gaccgcggcg
     2281 gctcccgtgg acgcccgcgc aggtaataac ctaccggtgg cgctttctct cagggaagcg
     2341 ccaccggatt cgcaggatat ctatggctcg agcattttcg ttcgcggcag agaccagtcc
     2401 gcaggtgcgg cagcttgcca acggaatcac actgctgacc gaacgcctgc cgcatgtgcg
     2461 ctcggtatcg ctgggtgtat ggatcacaac cggttcagcg aatgagccgc cccatctcaa
     2521 cggcatctcg catttcctgg agcatctgct cttcaaaggc accacgacgc gcacggcccg
     2581 ccaattgatg gaagccattg aggctcacgg gggccacctc aacgcctata ccgcgcgcga
     2641 gtacacctgc gtttacgcaa aaacgctgga cagctatctc gacacggccg tcgagatcct
     2701 cggcgacctc gtctgcgatt cacagctcta cgattttgaa aaagagcgca acgtcattct
     2761 ggaagaaatc gcatccaatg acgacattcc tgaggaattg atccatgacc tgttcatgag
     2821 caacatcttt cccaggcact ctctgggcgc tcccatctca ggcgaattga aatcggtgga
     2881 acaactctcc caggacgacg tgcgcgccta ctatgccaca tggtaccgcc cggggaatat
     2941 ggtgatctcg gcggcgggcc atttcgacga tgacgaaata ctggacgcca tcgggaaagc
     3001 cttcgtttcg ctgccggtgg gtgccgctat ggccgtaccc gcttacgaag cgcccacact
     3061 gggcgccggc atccgtgaat accggcggaa gattgcccaa agccactact gcatcggttt
     3121 tcccggcccg gccacggaca gtcccgaccg cttcgtctac gatgtcttgt gcaacgcact
     3181 ggggggcggg tcgacgtcgc gcctgttcga gcgaatacgc gagcaggaag ggctgtcgta
     3241 tgcgatctat tcgttccaat cggcgtatta ccgggcgggg ctgctgggaa tctatgccgc
     3301 agtagcgccg gaaaacctga accgcgcgct ttcagtgacc tttgaagagc ttcgcggcgt
     3361 ccgcgacaac ggtctgcccg ccgtggagat cgagcaaaat cgcgaacagc tcaagggcag
     3421 cttactcatg gccctcgaag atacgtttca tcgcatgagc cgcatggcaa aatcgcacat
     3481 ttactatggc cgcgtggtgc ccgtggaaga agtgatcgct caggttgatg cggttacagc
     3541 ggaggcggtc caggagcttg ccgatcggat tctacgggcc tcaaaatgca tggtaacggt
     3601 gctgggaccg aaaaacaggt tgcatctgcc ggagatcccc ctgtgaaccc tccgctgcgc
     3661 gtacaaatca tccgcgaacc cggcacggag gacatcgccc tgccccagta cgagacggcc
     3721 cacgccgcag gcatggatct gcgggctgcc gtgccggaac ccgtgctcct gcggcccggc
     3781 gaacgggccc tggtgcctgc gggcctgcgc atcgccctgc cgcccggata cgaagcccag
     3841 attcggcccc ggagcggtct ggccattcga cacggactga cgctgctgaa ttcgccggga
     3901 accatcgatg cggattaccg gggcgagatc tgcatcatca tcgccaatct cggacaggaa
     3961 ccgttcacca tcgagcgcgg cgatcggatc gcgcagatga ttatcgctcc cgtcacccgc
     4021 gtcgaatggg atgtagtgaa tgcgctcgat actacgtccc gaggcgacgg cgggtttggc
     4081 agcaccggca ggcagtgacg cgcgggcgcg gcaagtattg gacccattct atcgcggtca
     4141 ggagcatgat gatgcatacg tttacggtta ttgcgggcgt tctggcggcc agcgcaccgg
     4201 cccaaccggc accgccagcc gacgtgcttg tcggcgtgaa ctatttcgcg ggctggtggg
     4261 aacctctccc caacaaatgg catgacgcaa ccggcgagga ctggcgcccg cgctatcccg
     4321 agcgcgtgcc cacgctgggc gagtacaaca cgcaagagac catggatcgc gagattatcg
     4381 cggcggcgtc ccacggcgtc gatttctttg ccatcctctg gtactacaac ccgcccggca
     4441 cggaggccga gccgaacgcg cggttcctgg aacgcggcgt gacgaatttc atcgcctccc
     4501 ccgaaaacca ccgcatgcgg ttcatgctcg aattctgcaa ccatcctcca ttcgaagttg
     4561 cgacggacga agactggacg cggtgcgttg agctgtggac cagctggatc cagcacccgc
     4621 agtacctgcg gatagacggc aaaccggtgt tcaaagtgca tggattccat catttctata
     4681 cgcagaacgg ccgggatccc gccttctgca agaaacgcct ggatcagctc cgccagtccg
     4741 tccgcgacgc gggcgcgggg gagatgctca tcggatgcgg agtcggcgag cacgatgtca
     4801 ttgccgaagg ccacccggcc gtctcgctct ttgatttcac cgccgtatac atgggggtgc
     4861 cgccgctgga acagcgcagc gaagattatc cctacaaaac attgcttgac cgtgcccagg
     4921 tgtcctggaa aactcacggc ggcgacgcca tcccccacat gccctatctg cccgcgggct
     4981 ggtcgccccg cccctggcat gatccccggc ccagcttcgc ctttcccacc cgcggcgaat
     5041 gggacggcgc gctcagtgac atcaaggcag cgctcaccac gacatcgatc ctgggcctgc
     5101 ccggccagaa ggccttcacc atctatgcct ggaatgagtt cggcgagggc ggcatcgtcg
     5161 cgcccactca gggtgaaggg accatgaagc tcgaaggcat ccaggccaac ttccatccct
     5221 gaggcgcatc gcctgccggc ttcatcatgt gacgcttctg ttggccccat ctgctcgctc
     5281 tcgcggcgcg ttgtatctgg tctgcgatta tgggatgctt gcgggggatc aaaaaacaag
     5341 gagtcgcgtc atgttgtttt ctgccgtgtg tgttgcttct gtctttgtgc tgactgcagc
     5401 ggaacccgaa gctctgtcgg atgcgccatt tccggatccg ttgctgttgg aaaatggaac
     5461 gcgcgtcaac acgcgggaag actgggaagc gcgccgtacg gaaatgcgcg atcttctgtt
     5521 gcgccacgaa tacgggagca tcccgaccgc gccggttccg attgcgcacg cggtaattct
     5581 gaccgaagag ctgcagaacg atgatgtgac gctgtttcgc gacgttcggc tggcggtggg
     5641 ctctgaaaac ccgttccccg ttcgggtcga agtctacctg cccgcgaaag gcccgggccc
     5701 gtttccaacg atcctctgcg tggggctgaa gtatccggag gtgcaggtga tcaacgggcg
     5761 cggatatgcc ttcgtttgct acgagaacga ggatttcgac ttcgataccc agggcggcga
     5821 caaggtcggg cctgcgcagg ccatgtttcc ggactgcgac tggggaagcc tcggcgcctg
     5881 ggcctggggc gcttcgcgtg tgctcgacta tcttctgacc ttgcccgaag tggatgcgca
     5941 acacgttgtg gtcaccgggc actcgcggag gggcaagacg gccctgcttg cgggggcgct
     6001 ggatgaacgg ttcgccatcg tcgtgccgaa cggctcgggc tgtggcggcg cggcagccta
     6061 ccggcgttac ggccccgaat gcgaaaccct ggaagccatc acgcttccct cacggttcga
     6121 aagctggttc caaaaggatt tcagccggtt tgcgggcaaa gaagccgagc tgcccttcga
     6181 ccagcatttt cttcgggccc tggtcgctcc ccgcccggtc ctgtcgaccg atgccttcgg
     6241 cgatctgtgg gccaatccca tcgggacagc cgcggcgtgg cgcgctgccc agcctgtgtt
     6301 cgattttctc gaggtcccgg agaataacgc gatgcatttt cgcgagggca agcacgcaca
     6361 tctccccgag gattacgagg ctctcctcga tttcagcgat caccatttct tcgggaaacc
     6421 gctcacgtgt acctacaatg aaggccccga ggagaagtag aaccggcggt cgctctgctc
     6481 acccgttgat cgaaggccag gccacacagg ggggcctggc ctgagcagtc cagtggtttg
     6541 cttcgggctt tattctgtat tcagcgtttt ggcggcgcgg cctcacgcac ggcggcgccg
     6601 tcggtatcgc tgagtatcag cagtgcaaac cgctccacct gatccagttc atcggtgttg
     6661 agcgagctgt aatcgaagcg tccgcgcaag tcggtatatc catccttgta aaagcggatc
     6721 tcgccgccct gcaagcgggc gtacaccttc acgtagactt ttgacagcgc ctggccggtt
     6781 tcgagacttg tgacccggag ctggccataa ttttcgatga cctgaaccgc gaggttggcc
     6841 gcgtaacagg tctgagtctt tgtcttcccg ccggcagcga tctcgatcag gacgttgctg
     6901 ctgctgaatt gagacggcag atcgaaggtc agggtggtct gcgaggcggg aagctcgcgc
     6961 tcctcagtaa agtttggccg gaccgttgag aaatggccgg cgccctgcct ggcgaaggga
     7021 tcgcgcgaga aaagaagctc gatgtccatg agatagtagg ccacgcggca ggccgtgagg
     7081 ttctggtagg cgatggtgac ctgcctggct tcgaccttga aatcgaagtc cggctcggtc
     7141 tgagcgagct gttcctggac ctgttcccgg ctcttggcat cgatcacggt cgtcgtggcg
     7201 gattggattt cccgcacctg agcggcgaca ttggcaaagc ggttgcgcca gcggtccacg
     7261 gggtacgatg catacgtacc ggcgatcgca gctgcttttt cgggttcttc ggtataaaac
     7321 gccgcgcagc aggccaggta atcgtattgg agccgcatat cgatcgcatc cgggcggact
     7381 ttgcccagga aggccaggcc ttcctcaatc cggtcctgga gcatcagata ataggccacc
     7441 gacatcaaat cgtgctggtc aagtgcgggg cggtgcgcca ggatttccag caggcgggtg
     7501 tattgcgagg cgagccgcgc gttgacgata acgcggcggg gacccagtct gtgcgcccgc
     7561 gcgttgacca acggactgta ttccatgtgt tggtaggttt tccggagcac cggatccagg
     7621 cggagcaggg ggctgtcgat ggccgcgccg cactggcccg cgaaatcgtc gcggaactgc
     7681 aggtattcgc gcccggcgtc cgtatcgttg tgcaagaggg catacgacca gagggttggg
     7741 gcaaaagccc ggcgctgccg caagatatcc agtgtgttgg cgaacacagc cggatctttc
     7801 atgcgccagg cgatcaaatc gaggttgatg gtgcggtcgc tggcggcatg gatgttctca
     7861 tttcgcaggt actgatagac ctcttcgtca gtgccgtgct gtgaaacata aggccaggat
     7921 gccgtatcga cggtgctgag tgcggcaacg accttcatgg cgacggattg cgcaaacgcg
     7981 gcgagtttgc cgtctttggc cacatggacc gggaaatgct cgaaaagacc ttcctccggg
     8041 aagtagaagt acgtttcaat cgtggccgta tgatacggct cgagtgcgag gctgtaggtt
     8101 ttgaggtatt gtgtgcgctc gactggaacg gccccttcgg ggatctgcag aaggacgtcg
     8161 agacgctggc gggacgacgt ggagttcgtc acaacggttt ggcacccgta gacgacctgg
     8221 gtgataaatt cgtcaacgac gaatttatca acgcgctcgt tgtcaaccag gcggaaacga
     8281 tcgtctgaac ggaaaaaatt ctggctgacc aggatcggag gagtatcctc cggcatatca
     8341 gcgggggctg cttccttgtg aaagacgacc gcgggtccgc cggccgtgag ggtgatccgg
     8401 gcggcgctgc gttcgacctg gtgttcgccg gcttcaaacg gaagatcgag tactgccagg
     8461 gcgaacatca cttcagtgaa gttgtgcgac gcttccgcca ggttgcccga caaaaagggc
     8521 gcggaggcat cgcgtcccgc gtagtcgcgc cagaacgcat taacggaaac ccggtccgcc
     8581 gtctgttgct cgacgggcag ttcgaagtaa ttgttttcga cccactcccg ggttttttcc
     8641 atggcggtgt acagggcccg gtatcggtca ctgcgttttt catctttcac aaaaacgcgg
     8701 cccggctccg gttttgcagc ttccgccggc gccgcctctt cggcaacgtc gagctgcata
     8761 gcaacggcgt cctcagcagc cggttccgca ctggtgctgg ttacttcaaa cgccccccag
     8821 tcgccgccca tatccattct ggcgccttgc ctgctcgcaa agagcgcccc gcccccgtat
     8881 ccgcgtgcca ggccgttgac atcaagcgag tggccgcgca aggcggtcat gaacaggtgg
     8941 ttgaaccggt cgacatcggg cggaatcagg ttgtattggt cggttatgaa acgcagggtg
     9001 ttttcgcgct cggcggcgac gcgctcggcg agcaagattc gctcgagcgt gttgagctgg
     9061 ctgtagcgcc agggggcgag gtatgccgcc agctcgtcgc cgagaagata gtggtcaagg
     9121 aaggttttgt cgagtttgtt ggcgataaag ggcttcacgg ccacatcgaa gaaagccgga
     9181 tcttttcgct gcaggaacag gttca
//
LOCUS       DAOPBQ010000064         9162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7144_length_9162_cov_3.739212, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000064 DAOPBQ010000000
VERSION     DAOPBQ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7144_length_9162_cov_3.739212"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..3706
                     /locus_tag="PKW60_03445"
     CDS             401..3706
                     /locus_tag="PKW60_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98515.1"
                     /translation="MRQRCLQRSGEILRTIVLLSLAFICPLISLPARALDQIFRQTDV
                     DLSTEAQLLANPAFDQGDAVAEKDGKGVQLPEWDFWGDGYVIVPGAGRNSSRAIQCSI
                     ADDTTECGAGQRIELNQEQPFPVVATAWSRAQEVSGSPDSGYSIYLDFIFQDGTTWWA
                     QTANFSTGTHDWEERHFIVVPKKPIRSVYVYLLFRSHVGTVYFDDVNLLELRGETYMF
                     EGVPVRPQPVRAPVEKVWIPYMQTRNGLQMALERKRARVCGLWVNQEKVGLNGPGFFA
                     RDTAAKSDFLAPWNWNFTQQDSTLVIKGDIEPLNLRITASMTPRDDRIQVSGVVEALR
                     SEERAVTVYFGLPVDDSGWFWCDDPRTSIPATATIYMNANNTVAGATGAMSKFPLGAI
                     YKDDPVASPRNRGVAIAVPMHEPRHYRIGFDARTGLFYVAFDFGLAPTATATPGKATF
                     DFVIYPFTARWGFRGALASYYSIYSDYYQRRAGVEGLWLPKTPVNAIEGPEDFFFAYY
                     SVRDNIGLNAQPGLLSFVYSDPTNVWVPMPENIPRTPDDAVGFVQSQKPMGSDQIASS
                     GILDITGRPVLAMKDTPWCNGAAFAVNPDTGLAGATNKGRHELDSIAQRLGSRGAPTL
                     PAWPPLDGGYTLDVGGGRGGSTAIRLETSRSTPRAGAGQRVVIEQTEPAPLEFRGFTK
                     TKPLKSRSDDQCVISVTLLDSQGQSRTERIQVAREKDDFAPVSQTFESTEPIARATIK
                     VLLCDAKGATAWFDDLFLGEAGSDENLLRDGAMEEEPVEAGGMGGVFVDNLSGWGSTI
                     NYNHAHFGQAELPLVYDSHTQKVGILNMFSTYEYLRALAEPFRKSGMLIMASGAFGFP
                     VPLVDIACQEVNWCREGRWYPTTDDTLISYRALANQKPICLLLNTDFDSFLREPILAD
                     LDQEVDPNIERQPSLIMAERYFQWALFYGMFPGFFSKDGDTDAFFEHPEWYNTLRPLF
                     VKYMPLILELSKAGWEPVTGAETVGTDRIYLERYGRGEQGQVFITSRYGDQRWQMSSE
                     EGRDVREQLDEPVTAEIDVNMAHIGFLGTDSFEVVDLLTDTPIEFRYDQSHLYYTITL
                     QPHSVHLIRIRPA"
     gene            4111..6063
                     /locus_tag="PKW60_03450"
     CDS             4111..6063
                     /locus_tag="PKW60_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPJ98516.1"
                     /translation="MASSHDRERERAMDLAEESREAEWTYPSFVEELFHGNFRWDLLH
                     PFPEQSPEDKRTGDEYINKIRRVLEAYIDPCEVDRSGEIPEEALLALADAGAFGLKIP
                     REYGGLGMSQTNYNRIMEFIASYCTSTAVCLSAHQSIGVPQPLKLFGSEEQKKKYLPR
                     LAKGAVSAFALTEPQVGSDPAKMRTRAELSEDGTYYTINGEKLWCTNGPCAEILVVMA
                     LTAPRIVRGRERPQVTAFIVESSEPGFEVVHRCSFMGIRGISNGLLRFTNVKVPAGNI
                     IGQPGQGLKIALTTLNTGRLTVPAAAVAVGKACMHFSQKWINERVQWGAPIGKHQPVA
                     RMIADMAADTFAMDSVNTLACAMVDQGGVDVRLEAAMAKYYCSETAWRLVDDFLQVRG
                     GRGFETAESLGMRGEEPIPTERMLRDARISRIIEGTTEIMRLFIAREALDAHVRRIMP
                     LMTGKGPRGRLFWDAVKFYARWYPRLWLPSAVSLNVRNLTPRNQDHVLYIARASRKLA
                     RTLFHTMAKYRQKLEREQLILQAFVDIGTDLFAMAAALSRAEFELKAYAGDETLQDLV
                     DLFCTNARRRIRDNFRKAKQTRGRAISRVANAFMAGKYRWMITDVYTDFPPASRPARM
                     TRPVELKRPETPAPQTEEHESPVANP"
     gene            6094..6600
                     /locus_tag="PKW60_03455"
     CDS             6094..6600
                     /locus_tag="PKW60_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HPJ98517.1"
                     /translation="MRLTRRSRALLLGMLVLAAGMFQVLWGMFFAGDAGRPVSPAVPP
                     AQNAPVSPAQAESATDQALQSAFQNQRSGVQVLGEGVVTRTLPDDLEGSRHQRFILRL
                     GSGQTLLISHNIDLAPRIGGLQAGDTVAFYGQYEWSAKGGVVHWTHHDPRGSHADGWL
                     KHLGRTYQ"
     gene            complement(6753..9017)
                     /locus_tag="PKW60_03460"
     CDS             complement(6753..9017)
                     /locus_tag="PKW60_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98518.1"
                     /translation="MNIPEHRHHSQTNQRNGSGTFGRVFAPCLVANLSVILFMRSTFV
                     VGQAGVAGAIAVLLFAQSIALLTALSAGAVFTNTAVRGGGAYYAISRALGSELGGAIG
                     LMLFLAQTMAVSFYALAFSIVATQTFPPLREQGLAVALIAAGCLFAATYVRARWTAQV
                     HYVILGVLGVSIFVFLTEAALRFSPENFTSNLWPSDISIRFGGTAGEGPSLWMLFAVF
                     FPAVTGLMAGANPPGELKEPPRNVSWGILAAMGIGLTLYLVQILISGGASSRDQLLGA
                     PCPAVAENALLQLGFLVAAGLFAATLSSGFVSLLGASRVLQAVARDNTVGALRSFAEG
                     SRDTDEPRRGIAGCALIALAVIFGARSSAGDAALDLVARMMTLFSLYAFATLNLAAFI
                     QDFRSNPLFRPRFRYFHWGTALAGSIGCIVAATAILWWQSLVTMALLGVLAWTIKRRE
                     LTDTLGDSRRGQVWRSTRNHLMQLTELEETPRKWRPAAVVFSENPERREALVMYSIWM
                     EAGRGLVFLANVLVGNFEENALHRATAARQLREFCRKRAINAFPVVVIDENLENGMTG
                     VMQALSMGPLRTNLAVFEWSDSPARLKAHLREFCRAKRLGMNVAAIHPGRVLIPQGPK
                     RVDIWWRGRKNGELMAILGHLLTCNWEWTNTRLRVLRQIGNETGRESALNDLTHLLRD
                     AHIEAETGVVVSEQPFAEILAHYSSDASCVFLGFEVPPAGHEHTWFHQYESLLLDTPA
                     TILVSSIGADDLMV"
BASE COUNT         1886 a         2823 c         2748 g         1705 t
ORIGIN      
        1 gagtccgccg cgagggggcc ttcattctgc tctcgtgcaa cagacataac gagttttcgg
       61 gcataccctc tcaattggaa cgaaaccgat tcagaaggga tataagcaag ggcgaagtct
      121 ctgcgtccgc ggtatgcgga gcggcgcttg ctccagggta cgggcttcat atagactgac
      181 tttgcgttcg tgcagactgc gctggctgcg cgggggtcgg acggccggaa cgggtaagcc
      241 ttcgaagtac cgggccttcc ttctctcttc cgccagagcg ggtgtctccc cgggcgctca
      301 ggccaggtcg ccatgcatga gaagcctttt gccgatgcgt caaggcgcac tcaggaaggc
      361 gggtgcgcgt cgagataagg caggattgga ctaatcgcat atgcgccaac gatgtcttca
      421 gcgatcaggg gaaattttac gcaccatcgt actgctttcg ctcgctttca tctgtccgct
      481 tatctctctt ccagcccgcg ctttagacca gattttccgc cagaccgacg tcgacctctc
      541 caccgaagcc caactactcg ccaacccggc attcgaccag ggcgacgcag ttgccgagaa
      601 ggacggcaag ggcgtacagc ttccagaatg ggatttctgg ggagacggtt atgtcattgt
      661 cccgggcgcc ggccgcaaca gcagccgcgc gatccagtgc tcgattgccg acgacaccac
      721 cgaatgtggc gccgggcagc gtatcgaact caaccaggag caaccctttc ccgtggtggc
      781 caccgcctgg agccgcgcgc aagaggtcag cggcagtccc gacagcggct attccatcta
      841 tctggacttc attttccagg acggcacaac ctggtgggcc cagaccgcca atttcagcac
      901 cggcacacac gactgggagg agcggcattt tattgtcgtg ccgaagaagc cgatccgttc
      961 cgtctacgtg tatcttctct tccgcagcca tgtgggcacg gtctacttcg acgatgtgaa
     1021 cctgctcgaa cttcgtggtg aaacctacat gttcgagggc gttccggtcc gcccccagcc
     1081 ggttcgcgcc cccgtcgaaa aggtgtggat cccttacatg cagacccgga acggcctgca
     1141 gatggccctc gagcgcaagc gtgcccgggt atgcggtctc tgggtcaacc aggaaaaggt
     1201 cgggctgaac ggtccgggct tctttgcacg ggacacggcg gccaaatccg atttccttgc
     1261 gccctggaac tggaatttca cccaacagga cagcaccctg gtcatcaaag gggacatcga
     1321 acccctgaat ctccgaatca cggcatccat gacgccacgc gacgatcgca tccaggtgag
     1381 cggcgttgta gaagccttgc gcagcgagga gcgcgcggtc accgtctatt tcgggctccc
     1441 ggttgatgat tccggatggt tctggtgcga tgacccgcga accagcattc cagcgacggc
     1501 gaccatttac atgaatgcca acaataccgt cgcgggcgcc acgggcgcca tgtccaagtt
     1561 tccgctcggt gcgatataca aagacgatcc cgtggcatcg ccgcgcaacc gcggcgtagc
     1621 tatcgccgtg cccatgcacg agccccggca ctaccgcatc ggcttcgacg cacgtaccgg
     1681 gttgttttat gtggcgttcg actttggtct ggcgcccacc gccacggcga cacccggcaa
     1741 ggccacgttt gattttgtca tctatccgtt caccgcccga tggggattcc gcggcgcgct
     1801 cgcctcgtat tacagcatct actcggacta ttaccagcgg cgggccggtg tggagggcct
     1861 ttggctgcct aagacccccg tgaatgccat cgaagggccg gaggattttt tctttgccta
     1921 ctattctgtc cgtgacaaca tcggcttgaa tgcgcaaccg ggtttgctgt cttttgtgta
     1981 ctccgacccg accaacgtct gggtgccgat gcctgagaac atcccgcgaa cgccggatga
     2041 cgccgtcggc ttcgtccaat cgcaaaagcc catgggcagc gaccagatcg ccagctccgg
     2101 aatcctcgac atcaccggcc gccccgtgct ggccatgaaa gatacgccgt ggtgtaatgg
     2161 tgcggccttc gcggtcaatc ccgataccgg gctggctgga gccaccaaca agggccggca
     2221 cgagctggac tcgatcgctc agcggcttgg cagccggggc gcccccacgc tgccggcgtg
     2281 gccgccgctt gatggcggtt ataccctgga tgtcggcggc ggtcgtggcg gaagtacggc
     2341 catccgcctg gaaacgtcac gcagcacgcc ccgggccggc gcaggccagc gggttgtgat
     2401 cgaacagaca gaacccgccc cccttgaatt ccgtggcttc accaaaacca aacccctgaa
     2461 gagccgttcg gacgaccagt gcgtcatctc cgtgacgctc ctcgattccc agggccagtc
     2521 gcgcaccgag cggattcagg ttgcacggga aaaggacgat tttgcgcctg tcagccagac
     2581 tttcgaaagc acggagccca tcgctcgggc caccatcaag gtactcctgt gtgatgccaa
     2641 aggcgccacg gcctggttcg atgacctgtt cctgggagaa gccggttcgg acgagaacct
     2701 ccttcgtgac ggagcgatgg aggaagaacc ggtcgaagca ggaggcatgg gaggtgtatt
     2761 cgtcgataat ctcagcggat ggggctccac catcaactac aaccacgccc atttcggcca
     2821 ggctgaattg ccgcttgtgt acgacagcca cacccagaag gttggcatct tgaacatgtt
     2881 ttcgacctac gagtaccttc gcgcgctcgc tgaacccttc cgcaaaagcg gcatgctcat
     2941 tatggcttcc ggcgccttcg gatttcctgt ccctctggtg gatatcgcct gccaggaggt
     3001 gaactggtgc cgggaaggcc gatggtaccc cactacggat gacaccctga tctcgtaccg
     3061 cgcgctcgca aatcaaaaac ccatctgtct cttgctcaac accgacttcg actcgttttt
     3121 acgggagccc attcttgccg acctcgacca ggaggttgac ccgaacattg agcgtcaacc
     3181 ctccctcatc atggcggaac ggtattttca atgggctctg ttttacggca tgttccccgg
     3241 attcttcagt aaggacggcg ataccgatgc cttttttgaa catcccgagt ggtataacac
     3301 gctccgcccg ctttttgtga aatatatgcc cctcatcctt gaactctcca aagcgggttg
     3361 ggagcctgtc accggcgcgg agacggtggg gaccgaccga atctatctcg aacggtatgg
     3421 gcgaggagag cagggccagg tgtttatcac ctcacgctat ggcgaccagc gctggcagat
     3481 gagttccgaa gaaggccgcg atgtgcgcga gcagcttgac gagcccgtca ccgccgaaat
     3541 cgatgttaac atggcccata tcggctttct tggaactgac tcttttgaag tggtcgattt
     3601 gctgacagac accccgattg agttccgcta tgaccaaagc cacctctatt acacgattac
     3661 acttcagccg cacagcgtcc acttgattcg cattcgcccc gcatgaaggc cgggcccggc
     3721 gccccgtccg gtgatcccgc tgatgaggat accgtccgcg cgcgcgctga aatccgcacg
     3781 cgcatggact gatggggtcg gggaggacat cccgctcttt tccacggggt agaaccgtgt
     3841 gcgcctcgaa ggcaggaagc ggggtctctg ggagatgata caagagtgcg catgaggccg
     3901 aaatctatca taactgactg ataacaagtc agttgtgatg aatgcgacta gacagacccg
     3961 aataggcggc ccggctcacg tgttgaggag gatgtggggt gcgttctttg acaaggccga
     4021 gcgcttactc tacactgacg cccaggccat aatttcgccg cgaacaagaa cttagaggca
     4081 ggcggcggtg gaggaagcag aaggtacgcc atggcaagca gtcacgatcg ggaaagagaa
     4141 cgggccatgg acctcgcaga ggaatcgcgc gaggcggaat ggacgtatcc cagtttcgtg
     4201 gaagagcttt ttcacggcaa tttccgatgg gatctcctgc atccgttccc tgaacaaagt
     4261 cccgaagata aacgtacagg cgacgaatac atcaacaaga tccgccgcgt tctggaagca
     4321 tacattgatc cctgcgaagt ggaccgatcc ggcgagattc ccgaagaggc cctcctggcc
     4381 ttggcggatg caggtgcatt tgggctgaaa atcccaagag aatacggcgg actgggcatg
     4441 tctcagacca actacaaccg tatcatggag ttcattgcca gttactgcac atcaacagcg
     4501 gtctgtcttt cggcccatca gagcatcggc gtgccgcaac cgttgaagct gttcgggagc
     4561 gaagagcaga aaaaaaagta cctgccccgc ctggccaaag gtgcggtttc cgcattcgcg
     4621 ctcaccgagc cgcaggtcgg gtccgacccg gccaaaatgc gcacccgcgc cgagttgtcc
     4681 gaagacggca cctattacac catcaacggc gaaaaactct ggtgtacaaa tggcccctgc
     4741 gccgagatcc tggttgtgat ggcgctgacg gcccccagga tcgtccgagg cagggaacgg
     4801 ccgcaagtca cggcgttcat cgtcgaatcg tccgaacccg gctttgaggt cgttcaccgc
     4861 tgcagtttca tgggaattcg cggcatctcc aacggcctgc tccgattcac gaacgtgaag
     4921 gtccccgccg ggaacatcat cggccagccg gggcaggggc tgaaaatcgc tctgactacg
     4981 ctgaataccg gccgcctgac cgtgccggcc gctgcggtgg ccgttggcaa agcctgcatg
     5041 catttcagcc agaaatggat caatgagcgc gtccaatggg gtgcgcccat cggaaaacac
     5101 cagccggtgg cccggatgat tgccgatatg gcggccgata ctttcgccat ggacagcgtg
     5161 aacaccctgg cctgcgcgat ggtagatcag ggcggcgtcg atgtgcggct cgaggcggcc
     5221 atggcgaaat attactgttc cgagacggcg tggcgcctcg tggatgactt cctgcaggtg
     5281 cgcggcgggc gcgggtttga aacggccgaa tctctgggga tgcgcggcga ggaacccatc
     5341 ccaaccgagc gcatgctgcg tgatgcccgc atctcgcgca tcatcgaggg caccactgaa
     5401 atcatgcggc tgtttatcgc ccgtgaggcg ctggatgcac atgtacgccg cattatgcct
     5461 ctcatgacgg gaaaagggcc caggggccgg cttttctggg acgccgtgaa gttttacgcc
     5521 cgatggtatc cgagactatg gctgccgtca gcggtatcgt tgaatgttcg gaacctgacg
     5581 ccccggaatc aggaccacgt gctctatatc gcgcgagcca gcaggaagct cgcgcgcacc
     5641 ctgtttcaca ccatggcgaa ataccggcag aagctcgaac gggaacagct cattttgcag
     5701 gcgttcgtcg acatcggaac cgatctcttc gctatggcgg ccgcgttgtc cagggccgaa
     5761 tttgagctca aggcatacgc tggcgatgaa accctgcagg acctggtgga tctcttctgc
     5821 acgaacgcac ggaggcgcat ccgggacaat ttcaggaagg cgaagcagac ccgcggtcgt
     5881 gccatttcca gggtcgcaaa cgcctttatg gcgggaaaat accgctggat gatcaccgat
     5941 gtctacaccg acttcccgcc agccagccgg cccgcccgta tgacccgtcc ggtcgaactg
     6001 aaacgcccgg aaacccccgc gcctcagacg gaggagcacg aatctcctgt tgcgaatccc
     6061 tagggccccg cgacccgtcc agaaggaagt cccatgcgat tgacccgtag gagccgggcg
     6121 cttctgcttg gcatgctcgt gctggcggcc gggatgtttc aggtcctgtg gggtatgttc
     6181 tttgcaggcg acgccggccg cccggtttcc ccggctgtcc cccccgcgca aaacgctcca
     6241 gtttccccgg cacaagccga atcggccacg gaccaggcgc tccagagcgc cttccagaat
     6301 cagcgcagcg gagttcaggt cctgggggaa ggtgtcgtga cccggacgtt gcctgacgat
     6361 ctggagggtt cacggcatca acgatttatc ctgcgcctgg gcagcggtca gacgttgctg
     6421 attagccaca atattgatct ggcgcctcga attgggggat tgcaggccgg tgacaccgtg
     6481 gcattttatg gccagtacga gtggagtgcg aaaggcggcg tcgtgcattg gacccatcac
     6541 gacccgcgcg ggtctcacgc agacggctgg ctgaagcacc tcggcagaac gtaccagtaa
     6601 cggtgggaaa cggccgcggg accggaattc ggatcctcgg agtccttgca acccgtgctg
     6661 tttgaacgga tgggcaggcg ccgaaagagg tcatggcggc acacggctgt ttcctcccgc
     6721 aacctctcga gccgggatca cgctggcctg cgctacacca tcaaatcatc agcgccaatc
     6781 gaggaaacca gtatggtcgc cggagtgtcc agcaggaggg attcgtattg atggaaccag
     6841 gtgtgctcat gacctgcggg cggcacttca aagccaagga acacgcagga tgcatccgag
     6901 gaatagtgcg ccagtatttc tgcgaaaggc tgctcactca ccacgacgcc tgtttcggcc
     6961 tcgatatgtg catcgcgaag caagtgagtc aggtcgttga gggcggattc acgcccggtt
     7021 tcgttgccaa tctggcgcag cacacgcagg cgtgtgttcg tccattccca attgcaggtg
     7081 agcaggtggc cgagaatggc catgagctcg ccgttttttc ggccgcgcca ccagatatcg
     7141 acccgtttcg ggccctgcgg gatgagtaca cgcccgggat gaatggccgc cacgttcatg
     7201 cccaggcgtt ttgcgcggca gaattcacgc aggtgcgcct tcaggcgcgc cgggctgtcg
     7261 gaccactcga aaacggccag gttcgtgcga agcgggccca tgctgagcgc ctgcatcact
     7321 ccggtcatgc cgttttcgag attttcgtca atgacgacaa caggaaatgc gtttatggct
     7381 cgtttccggc agaattcgcg caactggcgc gccgccgtgg cgcggtgcag ggcgttctct
     7441 tcaaaattgc ccaccagcac gttggccaaa aataccaaac cgcggccggc ctccatccaa
     7501 atggaataca ttaccagggc ttcgcggcgt tcgggatttt ccgaaaaaac caccgccgca
     7561 ggccgccatt tcctgggcgt ttcctccaat tcggtcagct gcatcagatg attgcgggtt
     7621 gaccgccaca cctggccacg ccgcgaatcg cccagggtat cggtcagttc gcgccgtttg
     7681 atcgtccagg ccagcacgcc aaggagcgcc atggttacga gggattgcca ccagagaatt
     7741 gcagtggctg cgacgatgca tccaatactg cccgccagcg ccgtgcccca gtgaaaatag
     7801 cggaaccgcg gccggaacag cgggttgctt cgaaagtcct ggatgaaagc cgccagattc
     7861 agcgtggcaa aggcatagag agagaaaagc gtcatcatgc gcgcaaccag gtccagtgcc
     7921 gcatccccgg cggacgatct cgccccgaag atcaccgcga gcgcgatgag cgcgcacccg
     7981 gcaattcccc gccggggctc gtccgtgtct cgtgaaccct cggcaaagga acggagcgcg
     8041 ccgaccgtgt tgtcgcgtgc gaccgcctga agcacccgtg acgcgcccag gaggctgacg
     8101 aatccactcg aaagcgtggc cgcgaacagg ccggccgcaa caagaaatcc aagctgcagc
     8161 agcgcattct ccgccacggc cgggcagggc gcgcccagga gctggtcccg ggagctggcg
     8221 cctccagaga tgagtatctg caccaggtag agcgtcagcc cgatccccat ggcggcgagt
     8281 atgccccagg acacgttccg cggcggctcc ttgagctcgc ccggcgggtt cgcgcccgcc
     8341 atgagccccg tcaccgctgg aaaaaagacc gcgaaaagca tccacaacga aggtccttcg
     8401 cccgccgtgc cgccgaaacg gatcgaaatg tcagacggcc agaggttcga cgtgaaattc
     8461 tccggtgaga aacgcagtgc tgcctccgtc agaaatacga aaatggatac cccgaggaca
     8521 ccgaggatga cgtagtgcac ctgggccgtc cagcgcgcgc gcacgtatgt cgccgcaaac
     8581 aggcagcccg cggcaatcag cgccacggcc agcccctgct cccgcaaggg cgggaaggtt
     8641 tgtgtcgcca cgatggaaaa cgcgagcgca taaaacgaca ccgccattgt ctgggccagg
     8701 aacagcatga ggccgatggc gccgccgagc tccgagccaa gcgcccggct gatcgcgtag
     8761 taggcgccgc cgccgcgcac tgccgtgttg gtgaagaccg cgcctgccga aagggccgtg
     8821 agcaacgcga tggactgggc aaaaagaaga acggcaatgg ccccggcgac gcctgcctgc
     8881 cccaccacga acgtggaccg catgaacaga atgacgctca aattcgccac gagacagggc
     8941 gcaaacaccc ggccaaaggt gccggatcca ttccgttgat ttgtctggct gtgatgccgg
     9001 tgctcgggaa tattcatgag tgaactgctt ctacgcgcat gggtgaagat agccggaaat
     9061 ttccgccggc ctcaggccac cacgccaaaa accggcttgc gggtcttcca ttggccccgg
     9121 gtgaagtccg gcacctccac gggaacactt cctctcgcga tc
//
LOCUS       DAOPBQ010000065         9105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7187_length_9105_cov_4.468287, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000065 DAOPBQ010000000
VERSION     DAOPBQ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9105
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7187_length_9105_cov_4.468287"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PKW60_03465"
     CDS             <1..436
                     /locus_tag="PKW60_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HPJ98519.1"
                     /translation="TGAGRLVDCWGAEAIYMGSPVNATAVARAAARHGKDVVLIPAGL
                     MTDPRFDAQEDWVGAAVVAMQAGGAIGEGADTFHVWRQRIEEEGIEALFQSAPHAEKL
                     RRIGLEDDIPFCARLDITAAVPRAEKRHPLGVILHNAAAAHT"
     gene            complement(447..3080)
                     /gene="mutS"
                     /locus_tag="PKW60_03470"
     CDS             complement(447..3080)
                     /gene="mutS"
                     /locus_tag="PKW60_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPJ98520.1"
                     /translation="MAKNWKSLGDVSERELTPMLRQYLEAKAESGDSLLFFRMGDFYE
                     LFFEDAIEASELLGLTLTSRDGADKGARIPMAGVPVRALDGYLARVIQAGRTVTICDQ
                     VEDARLAKGVVRRAVTRTVTPGTVLEPDLLDAATNNYLAAVVLDGPSAGLALADVSTG
                     ELLAAQLDVAPAKTLRDELIRMAPVEVLFPEGADPQVLDELRSQFESMRFTERPRDDF
                     DAKYTRELILDEFGLSTLKGLELEDAPEATGCAGAVLAYVRATQRDGVPHLRPPRRYS
                     PSSFVVIDGNTQRNLELVESLRDKSRRGTLIATLDRTRTSMGGRKLRHWILHPLLDLN
                     AVHARLDAVEELFNDASLRLRLAEILKGVADVERLLGRLTSRSGNARDVKALGESLAY
                     LPEIRAAIAPCQTALLREIHGGMDELRDVAQVAASAIVDDPPLVLNEGRIIRDGYDAE
                     LDRLRELVRGGRDWIADLQRQEVERTGISNLKVGYNKVFGYYIEVSKAQVGKVPETYH
                     RKQTLVNAERYITPELKSREEEIVNAQERMNDLEYALFCRIRDEIGAQAGRIQATADA
                     LAVLDALLSLADTAAAKNYCKPVLDGSDRIEVRDGRHPVVEDLMPQGKFVPNDTVLDP
                     AEAFLQIVTGPNMAGKSTYLRQVALITLMAQMGSFVPAAEARIGVVDRIFTRVGASDN
                     LVRGESTFMVEMIESATILNSATERSLIVLDEIGRGTSTFDGISIAWSVAEYIHDRVR
                     AKTLFATHYHELTELASKLDRVKNLNVVVREWSGKVIFLYRIAEGGADHSYGIQVAKL
                     AGLPREVIERAHEILAALESGSTANIGLPQQLQLFAPASPAPKPSEVEQELRRVNPDD
                     LSPREAQELLYHLKHLAGREE"
     gene            complement(3228..3602)
                     /gene="rplQ"
                     /locus_tag="PKW60_03475"
     CDS             complement(3228..3602)
                     /gene="rplQ"
                     /locus_tag="PKW60_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPJ98521.1"
                     /translation="MRHRKAGRRLGRTTAHRKATVKNMAVALFTHNAIETTLAKSKEL
                     RGFAEPLITMAKKDTVYNRRRAFAALRDKGAVSRLFNEIGPAFAERPGGYTRILRLGN
                     RLGDASSMARIELVGLGEYEEE"
     gene            complement(3686..4690)
                     /locus_tag="PKW60_03480"
     CDS             complement(3686..4690)
                     /locus_tag="PKW60_03480"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPJ98522.1"
                     /translation="MIAKDFVTPKFVKSDEGASHQYARFLVEPFERGYGTTVGNSLRR
                     VLLASLEGSAVTAIRIEGIHHEFSSIPGVMEDATDVVLNFKRCQLRLNREEPVIFSFK
                     HKGSGAVTARDVFKEHDVEVFNPDLVIFTPTSDSAQIEMEIKVARGRGYVTAESFELE
                     HAPLGTIYLDANFSPVTRVNFLVEDARVGHRTDYDRLILEVWTNGSITPQKALEEASN
                     LLIEHLKIFVQQPEEMQQDAAGGSAADPELTRKLARPVEELELSVRAANCLKAAGIKT
                     IGDLVKRTEAEMLRFHNFGKKSLDEIRSLLETMDLHLGMGSSDDADVAETVEETSETT
                     "
     gene            complement(4778..5110)
                     /gene="rpsK"
                     /locus_tag="PKW60_03485"
     CDS             complement(4778..5110)
                     /gene="rpsK"
                     /locus_tag="PKW60_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPJ98523.1"
                     /translation="MAHIQATFNNTVVSITDNQGNVVSWSSAGQVGFSGSRKSTSFAA
                     QVAAEAAARRALEAGLREVRVQVNGPGAGRESAIRAIQAAGLNITLIRDVTPIPHNGC
                     RPPKRRRV"
     gene            complement(5191..5556)
                     /gene="rpsM"
                     /locus_tag="PKW60_03490"
     CDS             complement(5191..5556)
                     /gene="rpsM"
                     /locus_tag="PKW60_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPJ98524.1"
                     /translation="MARVVGVDLPREKRVEAGLTYIYGIGPTRSKEILAETNISPDTR
                     VRDLTDEEVSRLSTAIQAQFKVEGDLRREVTANIKRLMDINCYRGLRHKRGLPVRGQR
                     THTNARTRKGKKGATIKKK"
     gene            complement(5589..5702)
                     /gene="rpmJ"
                     /locus_tag="PKW60_03495"
     CDS             complement(5589..5702)
                     /gene="rpmJ"
                     /locus_tag="PKW60_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPJ98525.1"
                     /translation="MKVRSSVKKICEKCKIILRHGRVRVICENPRHKQRQG"
     gene            complement(5753..5971)
                     /gene="infA"
                     /locus_tag="PKW60_03500"
     CDS             complement(5753..5971)
                     /gene="infA"
                     /locus_tag="PKW60_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPJ98526.1"
                     /translation="MAEEAEHTVDGTVAEALANGVFRVELADRQVVLAHISGKDSIRF
                     VRVLPGDRVRVALSPYDHNRGRIVERYR"
     gene            complement(5993..6763)
                     /gene="map"
                     /locus_tag="PKW60_03505"
     CDS             complement(5993..6763)
                     /gene="map"
                     /locus_tag="PKW60_03505"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPJ98527.1"
                     /translation="MIALRDENEIATLREANRIVADTLVTLAGHVRPGVTTRELDALA
                     EECIRGAGATPSFLGYRGYPNCTCISVEDVIVHGIPGDRALREGQVVSIDVGAYYQGY
                     HGDAALTVGCGPVDDLRQRLMDATDRALAAGVQAARAGNYLVDISRAVQRVCEQAGFS
                     VVRDFVGHGIGRKMHEEPQVPNFDTGARGPLLRAGMVLAIEPMVNAGQAGVKVLRDGW
                     TAVTIDGKPSAHFEHSIVVRDGAPEILSATPRLVWGQR"
     gene            complement(6767..7438)
                     /locus_tag="PKW60_03510"
     CDS             complement(6767..7438)
                     /locus_tag="PKW60_03510"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPJ98528.1"
                     /translation="MGRRIVMLGAPGAGKGTQAVRIAKRLAVPHISTGDIFRAHLKNG
                     TAIGQQVQPYLDAGQLVPDELTCAIVADRLQADDCQGGYVLDGFPRSVPQAETLTRLL
                     RDHGDDLDMAIDIAVPDEEIVVRLASRRFCPKCGKIFNLKFSPSSGGKKCDALGCGGT
                     LERRSDDEEETIRERLRVYHETTEPIIGYYESQGILRTVPAAQASADDVFAKVEELLG
                     TTGAA"
     gene            complement(7468..8796)
                     /gene="secY"
                     /locus_tag="PKW60_03515"
     CDS             complement(7468..8796)
                     /gene="secY"
                     /locus_tag="PKW60_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPJ98529.1"
                     /translation="MSQPVEALRNAFKIPELKSRIIFTLIMLAVYRLGAHVPTPGVDG
                     GALSGAMGRGGLLSFYDMFSGGAFSNATVFALGIMPYISASIIIQLLTAVWAPLERLA
                     KEGAEGQKKITEYTRYGTIGLCIVQSLFVASYLVKLNSPQLVIVPNPSPFFFVLCVIS
                     FTTGTAFIMWLGEQISEHGVGNGISLIIFVSIVSRMPAAMYSLVQQVRHGDLTVFEGF
                     LLIVVMGLVVAGVILVTTGQRRIPVQYPRQVKGRRVTAGGRNYLPLRVNQAGVIPIIF
                     ASSILMLPSMIMNAVKSPAAQAFFQHWFEYDSILYNLIYAALIIFFCFFYTAITFNPV
                     EIAENLKKYGGVVMGVRPGKATAEYLNRVMTRITLVGAVFLAGVALLPKFVNNGLGVQ
                     DFNVSQFFGGTSLLILVGVALDTVRQVEQHLIMRNYDGFMKSGRKTPGRR"
     gene            complement(8800..>9105)
                     /locus_tag="PKW60_03520"
     CDS             complement(8800..>9105)
                     /locus_tag="PKW60_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010496730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HPJ98530.1"
                     /translation="SRFAMAVVNVAALEKTFDAGAEVTPASLAKAGLVRPCKGGVKVL
                     GGGELSKKLQVKVNAVSASARAKIEAAGGAVELFEVPASRAKTRRGNGRGAKARAEE"
BASE COUNT         1634 a         3085 c         2585 g         1801 t
ORIGIN      
        1 cacgggggcc ggaaggctcg tcgactgctg gggggccgag gccatttaca tgggctcgcc
       61 cgtgaacgcc accgcggtgg cccgcgcggc ggcccgccac ggaaaagacg tggtactcat
      121 ccccgcgggt ctcatgaccg acccccgctt tgatgcacag gaggactggg tgggcgccgc
      181 cgtggtcgcc atgcaggcag gaggcgccat cggagaagga gccgacacgt ttcacgtctg
      241 gcgccagcgc atcgaagagg aagggatcga agctctgttt cagagcgcgc cccacgcgga
      301 gaaactacgc cgcatcggcc tggaagacga catccccttc tgcgcccggc tcgacattac
      361 cgcggccgtg ccccgtgccg agaaacggca tccgctgggc gtaatcctgc ataacgccgc
      421 ggccgcgcac acgtgacggt gcgcgattac tcctctcgtc cggcaaggtg cttcaggtga
      481 taaagcaatt cctgggcctc acgcgggctc aggtcatcgg ggttgacgcg ccgaagctcc
      541 tgctccactt cgctgggttt tggcgccggt gacgccggag caaacaactg caactgttgc
      601 ggcaggccga tattcgcggt actgcccgac tccagtgccg cgaggatttc gtgtgcgcgc
      661 tcaatcacct cccggggcag gccggccagc ttcgccactt gaatgccgta actgtggtcg
      721 gcgccacctt ccgcgatccg gtagagaaag atcactttgc ccgaccattc ccgcaccacc
      781 acgttgagat tcttgactcg atccagtttg ctggccagct cggtcaattc atggtagtgt
      841 gttgcaaaga gcgtcttggc ccggacccgg tcgtggatat actcggcgac ggaccaggcg
      901 atgctgatgc cgtcaaaggt gctggtgccc cgcccgatct cgtccagcac gatcaggctg
      961 cgctccgttg ctgaattcag gatcgtggcg ctctcgatca tctcgaccat gaacgtgctt
     1021 tcaccacgga ccagattgtc actggcgccc acacgcgtga agatgcggtc gaccactccg
     1081 atgcgggcct cggcggccgg cacaaaactg cccatttgcg ccatcagcgt gatgagcgcc
     1141 acctggcgga gataggtcga ctttcccgcc atgttcggtc cggtcacgat ctgcaggaac
     1201 gcctcggcgg gatccaggac tgtatcattg ggcacgaact ttccctgcgg catgaggtcc
     1261 tcgaccacgg ggtggcgtcc gtcgcgcacc tcgatccggt ccgaaccgtc gagcaccggc
     1321 ttgcagtagt tctttgccgc ggccgtgtct gccagcgaca acagcgcatc cagaaccgcg
     1381 agcgcatccg cggtcgcctg gatgcgccca gcctgggcgc cgatttcatc gcgaatgcgg
     1441 cagaagagcg cgtattcgag gtcgttcatg cgttcctggg cgttaacgat ctcctcttcg
     1501 cgcgatttca gctcaggggt gatgtatcgc tccgcattaa cgagcgtctg cttgcggtga
     1561 tacgtctccg gcactttgcc gacctgggct ttcgagactt cgatatagta gccaaagact
     1621 ttgttgtacc cgaccttgag attcgagatg cctgtccgtt cgacttcctg ccgctggaga
     1681 tcggcgatcc agtcccgccc tccgcgcacc agttcgcgga gccggtcaag ctccgcatcg
     1741 tatccgtcgc ggatgatccg gccctcgttc aatacgagag gagggtcgtc cacaatggcg
     1801 ctggcggcca cctgcgccac gtcgcgcagc tcatccatgc cgccatgaat ctcgcggagc
     1861 agcgcagtct ggcagggtgc gatggccgca cggatctccg gcagatacgc cagcgattcc
     1921 cccagtgctt tcacatcgcg tgcgttgccc gaacgcgagg tcagccggcc caggagacgt
     1981 tcgacgtccg cgacgccttt caatatttca gcgagccgca gccgcagcga cgcatcattg
     2041 aacaactctt cgactgcatc gagccgcgcg tgcaccgcgt tcagatccag cagcgggtgc
     2101 agtatccagt ggcgcagctt gcgcccgccc atgctcgtgc gggtgcggtc cagggtggcg
     2161 atgagggttc cacggcgcga tttgtcgcgg agcgattcga ccagttcgag gttccgctgg
     2221 gtattgccgt cgatcacgac aaaactcgac gggctgtacc gccgcggcgg gcgcagatgg
     2281 ggcacgccat cacgctgtgt cgcgcggacg taggccagca ccgcgcccgc gcacccggtg
     2341 gcttccggcg catcttcgag ctcgaggcct ttcagcgtgc tcaagccgaa ttcatcgagg
     2401 atcagttccc gcgtgtattt cgcatcgaaa tcgtcgcgtg gccgttcggt aaagcgcatg
     2461 gattcgaatt gcgaccgcaa ttcgtccagg acctggggat cggcgccctc ggggaacagc
     2521 acctcgaccg gcgccatgcg gatcagctcg tcgcgcaggg tcttcgcggg ggcaacgtcg
     2581 agctgggccg ccagcaactc ccccgttgat acgtcggcca gggccagccc cgccgacggc
     2641 ccatcgagca caaccgcggc cagatagttg ttcgtcgccg cgtcgagcag atcgggttcg
     2701 agcacggtgc ccggggtgac cgttcgggtg acagcgcggc gcaccacgcc tttggccagt
     2761 cgggcatctt ccacctggtc acagatcgta acggtccgcc cggcttgaat cacccgcgcg
     2821 agatatccat cgagggctcg caccggcaca ccggccatgg gaatccgcgc gcctttgtcc
     2881 gcgccatcgc gcgacgtgag cgtcaggcca agaagttcgg aggcttcgat cgcgtcttca
     2941 aaaaagagct catagaaatc gcccatccgg aagaacagca gcgaatctcc gctttccgct
     3001 ttggcttcaa ggtattggcg aagcatgggc gtcagctcgc gctctgacac gtcgcccagc
     3061 gatttccaat tcttggccat agggcgtgat tatagcagcc tgacacagcc ggccgcaccg
     3121 gtcacggcgt gtctgcggcc ccttccccga acggtcgtca ccaacagaaa cgcacaagag
     3181 cggccgcttt ggcccggctc ctgtgcgtga cacttgccaa gcgcgggtta ttcttcctcg
     3241 tactcgccca gacccaccag ttcgatccgc gccatggacg aggcgtcgcc gaggcggttg
     3301 cccagccgaa ggattcgcgt atacccgccg gggcgctctg cgaacgccgg gccaatctca
     3361 ttgaacagcc ggctgaccgc ccctttgtcg cgcaacgccg caaacgcgcg ccggcggttg
     3421 tataccgtgt ctttcttggc catggtaatg agcggttccg cgaagccgcg caactctttc
     3481 gatttcgcca gcgtggtctc aatggcgtta tgagtaaaca gggccacggc catgtttttc
     3541 acggtagctt tgcggtgcgc cgtcgtgcgg ccaagccgtc ttcctgcttt acgatgcctc
     3601 atgattgcaa ttcctattcc tcttgctgtg cccggggccc tcccagcccc tccctagccc
     3661 aaatactacc agaaacggcg cgccgtcagg tagtttcact cgtctcctca actgtctccg
     3721 ccacatcggc atcatcgctg ctgcccatgc ccaaatgcaa gtccatggtc tcgagaaggc
     3781 tgcgaatctc atccagtgat tttttgccga agttgtgaaa ccggagcatt tccgcttcgg
     3841 tgcgtttcac caggtcgcca atcgtcttaa tccccgcggc cttcaggcaa ttcgccgcgc
     3901 gcacgctcag ttccagttcc tcgaccggcc gcgccagttt gcgcgtcagc tccggatcgg
     3961 ctgcactgcc gccggcagca tcctgctgca tttcctccgg ctgctgcaca aaaattttca
     4021 aatgttcgat caacaggttc gacgcttctt cgagggcctt ctgaggggtg atcgatccgt
     4081 tcgtccacac ctcgagaatc agccgatcgt aatccgtccg gtgtcccacg cgcgcgtctt
     4141 ccacgaggaa gttcacccgg gtgaccggcg aaaagttcgc gtcgagatag atggtgccca
     4201 gcggggcatg ttccagctcg aagctctcag ccgtcacgta cccccggccg cgcgccacct
     4261 tgatctccat ctcgatctgc gccgaatccg atgtgggcgt gaagatcacc aggtccggat
     4321 tgaatacctc aacgtcgtgc tctttgaaca cgtcgcgggc cgtcaccgca ccgcttccct
     4381 tgtgcttgaa cgagaaaata acgggctctt cccggttgag gcgcagctgg caccgcttga
     4441 aattcaggac cacgtcggtg gcgtcttcca tgacgcccgg tatcgacgaa aactcgtgat
     4501 ggatgccctc gatccggatc gcggtgaccg ccgaaccttc cagcgacgcc agcagcaccc
     4561 gccgcaggga attgcctacg gtcgtgccat agccccgttc aaacggttcc acgagaaagc
     4621 gcgcgtactg atgcgatgcg ccttcatccg acttcacgaa ctttggtgtc acaaagtctt
     4681 ttgctatcat tcgatttcgc ctcccggcaa cgaggtcagt tttctcgatt ctacttccgt
     4741 ttcctggata cccatgcccg gcgtgcccgg cagcacgtta cacacgtcgc cgtttcggag
     4801 gccggcagcc gttatgcggg atgggggtaa catcccggat cagcgtaatg ttcaaccccg
     4861 ccgcctggat cgcgcggatg gccgattcgc ggcctgcgcc cggtccgttc acctggaccc
     4921 gcacctcgcg cagcccggcc tcgagagccc ggcgcgccgc cgcttccgcg gccacctgcg
     4981 ccgcaaacga cgtgctcttt cgcgaaccgg agaacccgac ctggccggcg ctcgaccagg
     5041 aaaccacatt gccctggttg tcggtgatcg acacgactgt attattgaac gtcgcctgaa
     5101 tgtgcgccat ccccgacgtg acattcaatg tggtgcggcg tttcttgcct ttgctcttgc
     5161 gtttttccgt ggccacgtag cctcctcctt ttatttcttc ttaatggtgg cgcccttctt
     5221 gcccttacgc gtgcgcgcat tcgtatgcgt ccgctgacca cgcacgggca agccgcgttt
     5281 atgccggaga ccccggtagc aattgatatc catcaatcgt ttgatgttcg ccgtaacctc
     5341 gcggcgcagg tcgccttcca ccttaaactg ggcctggatg gccgttgaaa ggcggctgac
     5401 ctcttcatcc gtcaggtccc gcacccgcgt gtcggggctg atgttggtct ccgcaaggat
     5461 ctccttcgat cgcgtcggcc caatcccgta gatgtaggtg agtcctgcct cgacgcgttt
     5521 ctcgcgcggc aaatcgacac cgactactcg tgccatgtta cccgttctcc ttatccggtg
     5581 cgttgcgttt agccttggcg ctgtttgtgg cgtggattct cacaaatcac ccgcacccgg
     5641 ccgtgacgca ggattatttt gcacttctca caaatctttt tcactgaact gcgaactttc
     5701 atctttcgtc tccctttcgg agcggtggcg cccggagttt ccggggcctc ccttatcgat
     5761 agcgctcaac aatccgcccc cggttgtgat catagggtga aagcgccacc ctcactcgat
     5821 cgcccgggag cacgcgcaca aatcgtatgc tgtccttgcc cgatatatgg gccaatacca
     5881 cctgccggtc agccagctcg acccggaaca cgccgttcgc cagcgcttcc gccaccgtgc
     5941 cgtccaccgt atgttccgct tcctctgcca tgccggtacg ggatacgctc cctcaccgct
     6001 ggccccagac gagccgcggc gtcgccgaca aaatttccgg tgcgccatcg cgcaccacaa
     6061 tgctgtgttc aaaatgcgcg ctcggcttgc cgtcgatcgt taccgccgtc catccatcgc
     6121 gcagcacttt cacgccggct tgtccggcgt tgaccatcgg ctcaatcgcc agaaccatgc
     6181 ccgcgcgcag caaggggccc cgcgcacccg tgtcgaaatt gggtacctgc ggctcctcgt
     6241 gcatcttgcg cccgatacca tgcccgacga aatcgcgaac cacgctgaac ccggcctgct
     6301 cgcatacgcg ctgtaccgcc cgcgagatgt ccaccaggta gttgcccgcg cgggctgcct
     6361 gcacgcctgc cgcgagcgcc cggtcggtcg catccatcag gcgctgccgc aggtcatcta
     6421 ccggaccaca gcccacggtc agcgcggcat cgccatggta gccctggtaa taggcgccca
     6481 catcgatgct cacgacctgc ccttcgcgca gcgcgcggtc gccgggtatc ccatgcacaa
     6541 tgacgtcttc caccgagatg cacgtgcaat tcggatatcc gcggtagccc aggaacgagg
     6601 gcgtcgcacc cgcaccgcga atgcactcct ccgcgagggc atccagctcc cgcgtcgtga
     6661 cgcccggccg cacatggccg gccagcgtca ccagcgtatc cgccacgatc cggttggctt
     6721 cgcgcagcgt cgcgatttcg ttctcgtcac gcagcgcaat caccggtcag gccgccccgg
     6781 tggttcccag cagttcttca acttttgcaa acacgtcgtc cgcgctggcc tgagccgccg
     6841 gaaccgtacg cagaatgccc tgactctcgt aatatccgat gatcggctcc gtcgtctcgt
     6901 gatagacacg caaccgttcc cgaatcgtct cttcctcgtc gtccgagcgc cgttcaagag
     6961 tcccgccgca gcccagggca tcgcacttct tgccgccgga cgagggactg aacttcaaat
     7021 tgaaaatctt tccgcatttc ggacagaagc ggcgcgacgc caggcggacc acaatctcct
     7081 cgtccggcac cgcgatatcg atggccatat cgaggtcatc gccgtgatcg cgcaggagtc
     7141 ttgtcagagt ctcggcctgg ggcaccgagc gcgggaaccc gtccagcacg tatccgccct
     7201 ggcaatcgtc cgcctggagc cggtcggcga caatggcaca ggtcagctca tcgggcacca
     7261 gctggccggc gtccagatag ggctgtacct gctgcccaat cgccgtgccg tttttgagat
     7321 gcgcacggaa gatgtcgccc gttgaaatat gcggcaccgc aaggcgtttg gcaatccgca
     7381 ccgcctgcgt ccctttgccc gcgcccggag cgcccagcat cacaatgcgt cgtcccatgc
     7441 gtatcatcgt ccttgcccgc cgtttggtta gcggcggccg ggcgtcttgc gcccgctctt
     7501 cataaacccg tcgtaattgc gcatgatcag gtgctgttcc acctggcgca ccgtatccag
     7561 cgccacaccc accaggatca gcaggctcgt gccgccgaaa aactgcgaga cgttgaaatc
     7621 ctgaacgccg aggccgttgt tcacgaactt cggcaacagg gcgacgcccg ccagaaatac
     7681 cgcgcccacc agcgtgatcc gcgtcatcac gcggttgaga tattcggcgg tagccttgcc
     7741 cggccgcaca cccatcacca cgccgccgta ctttttgagg ttttccgcga tctcgaccgg
     7801 attgaacgtg atcgcagtat agaaaaagca gaaaaagatg atcagggctg cgtatatcag
     7861 gttatacaaa atcgaatcgt attcgaacca gtgctggaag aaggcctgtg ccgccggcga
     7921 tttcaccgcg ttcatgatca tgctcggcag catcaggatt gagcttgcaa aaatgattgg
     7981 aatcacgccc gcctggttca cccgcagcgg cagataattc cggccgcccg ccgtgacgcg
     8041 gcgccctttc acctggcgcg gatactgcac ggggatccga cgctggcccg tcgtcaccag
     8101 gatcacgccc gcgacgacca gccccatcac gacgatcagc agaaagccct caaagactgt
     8161 aagatcgcca tggcggacct gctgaaccag gctgtacatc gccgccggca tccgggacac
     8221 aatgctcaca aagatgataa gcgagatccc gttgcccacc ccatgttcgc tgatttgttc
     8281 gcccagccac atgatgaagg ccgtgcccgt tgtaaacgaa atcacacaca aaacaaagaa
     8341 aaacgggctc gggtttggaa cgatgaccag ctgcgggctg ttcaacttga ccaggtagga
     8401 cgcaacaaac agcgactgca caatgcacag cccgatcgtg ccgtagcgcg tgtattccgt
     8461 gatcttcttt tgcccttccg cgccttcttt cgccagacgc tccaggggcg cccacaccgc
     8521 cgtcagcaat tgaatgataa tcgacgcgct gatgtacggc atgatgccca gcgcaaagac
     8581 cgtcgcgttg ctgaatgcgc cgcccgagaa catgtcgtaa aagctcaaca ggccgccccg
     8641 gcccatggcg ccgctcaagg cgccgccatc cacgcccggc gtcggaacat gcgcccccag
     8701 gcggtagacc gcgagcatga tcaacgtaaa gatgatgcgg ctcttcagct cggggatctt
     8761 aaacgcgttt cggagagctt ctacaggctg cgacactgat tattcctccg cccgcgcttt
     8821 cgcaccgcgc ccgttgccgc gtcgggtctt cgccctgctc gcgggcacct caaacaattc
     8881 cacggcgccg ccggccgcct caatcttcgc gcgggccgat gcgctcaccg cattcacctt
     8941 gacctgcagt tttttcgaga gttcaccgcc gccgagcact ttcacgccac ccttacaggg
     9001 gcgcaccagg cccgctttgg ccagcgacgc cggcgtcact tccgcgcccg catcaaatgt
     9061 cttctcgagt gcggccacgt tcaccacggc cattgcgaac cggct
//
LOCUS       DAOPBQ010000066         9105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7188_length_9105_cov_3.663204, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000066 DAOPBQ010000000
VERSION     DAOPBQ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9105
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7188_length_9105_cov_3.663204"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="PKW60_03525"
     CDS             complement(<1..1735)
                     /locus_tag="PKW60_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPJ98531.1"
                     /translation="MTEEQAERDEEPRGLEGALARKPRGCGGIFFMLMLVLAAGWGVG
                     LGMFVWMLDDARANIQAVDDFRPKVGSRIYSSDGMLLGEYTVEARQLVRLSEIPLHVQ
                     KAFIATEDHPFYVHKGVRPLAILSALKDAVRTGSVRGASTITMQIVRNITEVTGVSAE
                     RTMQRKAKEAFVALQLEREFTKDEILELYLNQIFLGGSANGVEAAARQYFGKSCKDVT
                     LAEAATLAGLSRSPNANRPDRFPEAAQQRRDIVLAQMLRHGFITRDDYLEAIATRLED
                     SVVSYEERMRRMQEGVGLWRPNRFQAPYFVEEVRQFTREHGYANREELLESGLQIYTT
                     VDMRLQRAAEKALEKRLAEIDEETLKTLTQRGQEDYFVPVSGALVCIDNRPGAEGYVR
                     AMVGGRDFDKEKYNTATQALRQPGSSIKPFVWLAAFQNGFTPSHIVTDERITLYDGLG
                     RPWSPANFTSDFKGPVTLRYALENSINIVSVKLVLQMGMPVVRGVLQRAGITSEINDS
                     HKWTIALGTPDVTVLDLCTAYATIARGGVLVRPTFVETIRDRDEFVRYKSSIHRERTL
                     DADVCYLTTYLM"
     gene            complement(1799..2227)
                     /locus_tag="PKW60_03530"
     CDS             complement(1799..2227)
                     /locus_tag="PKW60_03530"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPJ98532.1"
                     /translation="MKTRYIIAIALYCAGIFWISSQPRPLGFRVPGEFGIDKLGHCGV
                     YAGLAGLVSVGLRRSGSAWSPRWQFWFPILFAALYGLSDEFHQLFVPRRSFTLGDLVA
                     NTLGGVAAQYFLWYVWWRPPRPAASPTEQVHVKPECPAKD"
     gene            complement(2274..3863)
                     /locus_tag="PKW60_03535"
     CDS             complement(2274..3863)
                     /locus_tag="PKW60_03535"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HPJ98533.1"
                     /translation="MMRRREFMQAVGTGLATAAIGGSAGAQAPNRSKRPNFVIILADD
                     MGFSDLGCYGSEIATPNLDGLAREGVRFTHFYSAARCCPSRASLLTGLYPHQAGMGGM
                     VTQGEAPAEEGPYQGYLNRSCVTFAEVLKSAGYRTYMSGKWHVGERPEHWPRQRGFDR
                     YYGLISGASSYWGILEQERGQRAFALDDERIVPGGESFYMTDAFTDYAVTWLEEQAGR
                     EEPFLLYLAYTAPHWPLHAWPEDIAKYRGTYVRGWDALREERYARLVSQGIIDPKWKL
                     SPRDPDVPPWEEAENQTDWALRMAVYAAMVDRMDQGVGRVLAALHRLGLEKDTLVMFL
                     SDNGGCHESVEGRNLNTPGTEPGEPGSFVAYRRPWANASNTPFRLFKHWVHEGGIATP
                     LIARWPGGISRRGELEHQVGHINDIMATCVDLAGASYPATFNGREITPCEGRSLAPVF
                     RGEQREPHDRLFWEHMGNRAVREGDWKLVAAKSGDWALYNLAEDRTELNDLADAMPER
                     AEAMLQAWDAWAERVGARKPA"
     gene            4010..5041
                     /locus_tag="PKW60_03540"
     CDS             4010..5041
                     /locus_tag="PKW60_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPJ98534.1"
                     /translation="MQRHTRLNPFFPAILLFAILSVSIARAGEEAAPVELVSATKIWD
                     QGNHNAFTDLIRFQDRWFCSFREGEAHVFGKDGTIRLITSPDGETWESAALLSEEGVD
                     LRDPKLSITPDGRLMLLAGGSVYRGKEFITRRPRVAFSSDGRTWGPLTPVCEQGDWLW
                     RVTWHNGTAYGFSRAPIPQDGIEATSEEWQLTLYSGTDGIHYTPVCKPTVPGKPNETT
                     VRFMDDDTMLALVRREGGNTHCWIGVAKPPYTDWSWHETGYRIGGPNFIILPDGSMWA
                     GGRFHPDGPKTILARFGAQTYEPVLTLPSGGDTSYPGFVWLEGLLWMSYYSSHEGKTS
                     IYLAKIRIK"
     gene            5274..7730
                     /locus_tag="PKW60_03545"
     CDS             5274..7730
                     /locus_tag="PKW60_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HPJ98535.1"
                     /translation="MKSLVVSLAFLFTLPCLATAVELPGPTSEGYLLPNRWRITPVGT
                     AIDTNDLLLNIEIAPDGKAAIAVHGGYNAHGLVVIDTASGTTAQTIPLPTAWVGLVWS
                     PDGARLYVAGGNNKSSKGIRAPIYIFGYQDGRLTDESIGTFTEEIAPGRIFWSGLAHH
                     PSKNILYAANRTNSYVVVFDTATGEILHRIPTELNPYELAITPDGATLYASNWGSDSV
                     SIIDTEAGAVRRTLAVGDNPNDLAIARDGRLFVCCSNDNTVTVIDPRKERPTESIVTS
                     LYPQAPEGSTPNALAIDHTQHILYIANADNNNVCVVSVEKPGESAVLGFIPVGWYPSS
                     VAVSADGETLYVGNSKGIGHSSANPRGPHSPLPPGEGAQGTTKTLMRGAISIIDVPRH
                     RRDLRKLTKQAYANCPYNDELLATARPETEQTVIPREVGKGSKQIKHVIYILKENRTY
                     DQVLGDLPQGNGDPRITIFGQEVTPNQHAIVETFVLLDNLYCDAEVSSDGHQWSNAAY
                     ATDFNEKSWPASYAGKSDSPRSPAIIPNAGYLWDQCARKGLTYRSYGEFARRVSDDGV
                     MEPSDARFTALHGHVAPNYLNWGARDYENAAEFIREFDEYEKNYDDPNPEKRLPNFIV
                     MGLPEDHTKGTRPGVPTPRAAVASNDYALGLIVERVSHSRYWPEIAFFVIEDDAQDGA
                     DHVDARRTTGYVISPYIKRGIVDSTFYTTCSMLRTMELILGLQPMSQYDAAANAMFAC
                     FTDTPDLTPFKHIEPNIDLQELNIKTAWGAEESMKMDFSDYDRAPMFELNEIIWKSVK
                     GPDSEMPVPVHRFNTASIQE"
     gene            7821..>9105
                     /locus_tag="PKW60_03550"
     CDS             7821..>9105
                     /locus_tag="PKW60_03550"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPJ98536.1"
                     /translation="MKSARNLLEEETVMRFLIILALATTIAAFCAPAEPVQLATDGKP
                     AVSVVVEPNAGSLVSGAADDLQSYVKALCGVELPRHDDGAAVDGKAIYIGRCAPAPSS
                     AFPPAESNPETYAITAQDGNLFLTGNHPPATSFAVYSFLQDYLGIRWFAPGPLWEYVP
                     EGQPGELTLDVANGVVTPDWSCRVWSGHDYAPSWNLWNRRSKTICDSPVPFRNMQNFL
                     HVIFAPEKYAETHPEYYPLIDGKRWIPPADYQNWRPCESNPDVLRITVEAAREFLDAH
                     PEHNAFSLAMDDIYRLCSCDNCRARDARPDDYENKRFSDRHYKFVNAVARELAKTHPD
                     KFVGTLCYHIARELPETVEQLEPNVFISMTQRVGEWWRPQRHADDIALTHAWRKRCSH
                     MSRYGYIGLGFLTPRVFPHAMAEGMKLDKSLGFEAV"
BASE COUNT         1763 a         3179 c         2484 g         1679 t
ORIGIN      
        1 ccatgagata cgtggtcaga tagcatacgt ccgcatcgag cgtgcgttct cggtgaatgc
       61 tgctcttata ccgtacaaac tcgtcccgat cccggatggt ctcgacaaag gtcggccgga
      121 ccagcacacc gccccgggcg atcgtggcgt aggcggtaca caggtcaagc accgtcacat
      181 ccggggttcc cagcgcgatg gtccacttat gagaatcgtt gatttccgag gtgatccccg
      241 cgcgctgcaa taccccgcgc accaccggca tccccatttg cagcaccagt ttcaccgaga
      301 cgatgtttat cgagttttcc agcgcgtacc gcagggtcac ggggcctttg aaatccgacg
      361 tgaaattcgc cggcgaccag ggccgtccca gcccgtcgta cagcgtgatc cgctcgtcgg
      421 ttacgatatg cgacggtgta aacccgttct ggaacgccgc cagccataca aacggtttga
      481 tgctcgaacc cggctggcgc agggcctgcg tggcggtgtt gtacttttcc ttgtcgaaat
      541 cgcggccgcc gaccatcgcg cgcacatacc cttccgcgcc aggccggttg tcgatacaca
      601 ccagcgcgcc cgaaaccggc acgaaatagt cttcctgccc gcgctgggtg agcgttttca
      661 gcgtttcttc gtcgatctcc gccagcctct tttcgagggc tttttcggct gcccgctgga
      721 gccgcatgtc gaccgttgta taaatctgaa ggccgctttc cagcagttct tcgcggttgg
      781 cgtacccgtg ttctcgcgta aattgccgca cttcctcgac aaaatacggc gcctggaacc
      841 ggttcggtcg ccacaaccca accccttcct gcatccggcg catccgttct tcatacgaga
      901 ccacagaatc ttccagcctg gtcgctatgg cctcgaggta gtcatcccgc gtgatgaacc
      961 cgtgccggag catctgggcc agcacgatgt cgcgccgctg ctgggccgcc tccggaaacc
     1021 ggtcgggccg gttggcattc ggcgagcgcg ataaacccgc cagcgttgcg gcctctgcga
     1081 gcgtcacgtc cttgcacgat ttgccgaaat actgccgcgc ggccgcttcg acgccgtttg
     1141 cactgccccc caggaagatc tggttgagat acagttcgag gatctcatcc ttggtgaatt
     1201 cgcgctcaag ctgcagcgcc acaaacgcct ctttggcctt acgctgcatg gtacgttcgg
     1261 ccgacacccc cgtcacttcc gtgatgttcc gtacaatctg catggtgatc gtactggctc
     1321 cgcgcacgct gcccgtccga accgcgtctt tgagcgcgct caaaatcgcc agcggccgca
     1381 cccccttgtg cacataaaac ggatggtctt cggtggcgat gaaggccttc tgcacgtgca
     1441 ggggaatttc gctcaggcgc acgagctgcc gcgcctcgac cgtgtactcg cccagcagca
     1501 tcccatccga ggaatagatc cggctgccca ctttcgggcg gaaatcgtcc accgcctgga
     1561 tattcgcccg ggcgtcgtcg agcatccaca cgaacatccc caggcccacg ccccatcccg
     1621 ctgcgagaac cagcatcagc ataaaaaaaa tgcccccgca gccgcggggt tttcgcgcca
     1681 gcgcgccttc cagtccgcgg ggttcttcgt cccgttctgc ttgctcttcc gtcatggcct
     1741 cgatcctatc atccgccccg gcatttctcc aaagcggtca ccgttcgtgc attccttttt
     1801 aatccttggc ggggcactcg ggtttcacgt gaacctgctc cgtgggcgag gctgccgggc
     1861 ggggcgggcg ccaccatacg taccacagga aatactgcgc agccactccg cccaacgtgt
     1921 tcgcgaccag gtcacccagc gtaaatgatc gccggggaac aaatagctgg tggaactcat
     1981 cgctcaaacc gtacaacgca gcgaacagaa tgggaaacca gaactgccag cgggggctcc
     2041 acgcggaacc tgagcgccgc aaccccacgg agaccagccc ggccagtccc gcatacacgc
     2101 cgcaatgccc cagcttgtcg atgccgaact cccccgggac cctgaacccc agcggtcgcg
     2161 gctgcgacga tatccagaat atccccgcgc aatacagcgc aatggcgatg atataacgag
     2221 ttttcacgcg aaatgtgccc ccggcgtgcc gtgggtctgt gccctggccg cgctcatgcc
     2281 ggtttccgcg ctccgacccg ttccgcccag gcgtcccacg cctggagcat ggcttccgcg
     2341 cgttccggca tcgcgtccgc caggtcattc agctcggtgc ggtcctcggc caggttgtac
     2401 agggcccagt cgccgctttt agctgccaca agtttccagt cgccttcccg cacggcccga
     2461 ttccccatat gctcccaaaa caggcggtcg tgcggctccc gctgctcccc gcggaatacg
     2521 ggcgccagcg agcggccctc gcacggcgtg atctcgcgtc cattgaacgt ggccggatac
     2581 gatgcgcccg ccaggtccac gcaggtcgcc atgatgtcgt tgatatgacc cacctgatgt
     2641 tccagttcgc ctcttcgcga tataccgccg ggccaccgcg cgatgagcgg cgtcgcgatg
     2701 cctccttcat gcacccagtg tttaaataac cggaacggcg tattgctcgc attggcccag
     2761 ggccggcggt acgccacgaa tgaacccggc tcgcccggtt ccgtgcccgg tgtattcaga
     2821 tttcgtccct cgacgctttc gtggcatcct ccattatctg agaggaacat caccagcgtg
     2881 tccttttcca ggcccaggcg gtgcaacgcg gccagcacgc gccccacacc ctggtccatg
     2941 cggtccacca tggccgcata gacggccatg cgcagggccc agtccgtctg attctcggcc
     3001 tcttcccacg gcggcacatc aggatcgcgc ggagacagct tccatttcgg gtcgataatg
     3061 ccctggctca cgaggcgtgc ataacgctct tcgcgaagcg cgtcccagcc ccgcacgtat
     3121 gtgccccggt acttcgcgat atcctcgggc caggcgtgca gaggccagtg cggcgccgta
     3181 taggccagat acagcagaaa cggttcttcc cgccctgcct gttcctcaag ccacgtgacc
     3241 gcatagtcgg tgaacgcgtc ggtcatgtaa aacgattcac caccgggcac aatgcgctcg
     3301 tcgtccagtg caaacgcccg ctggccgcgc tcttgttcca ggatgcccca ataactgctc
     3361 gcgccgctga tcagcccgta gtaccggtca aatccgcgct gccggggcca gtgttccggc
     3421 cgctcgccga catgccactt gccggacatg tacgtccggt accccgcgct cttcaacacc
     3481 tccgcaaacg tcacgcacga gcggttcaga tacccctggt acggaccttc ttccgccggg
     3541 gcctccccct gcgtcaccat cccgcccatg cccgcctggt gcgggtaaag accggtcaac
     3601 aacgacgccc gcgacggaca gcaccgggcc gccgaataga aatgcgtgaa ccggacgccc
     3661 tcccgggcca gaccgtccag attgggcgtt gcaatctcgg aaccgtagca gcccaggtcg
     3721 ctgaacccca tgtcgtccgc cagaatgatc acgaaattgg ggcgtttgga acggttcggc
     3781 gcctgcgccc cggcgctccc gcctatcgcg gcggttgcca gaccggtccc taccgcctgc
     3841 atgaattccc gtcggcgcat catcgtcctc cactcgcgga ctccgggttt agtgaatcat
     3901 cgccccgcca ggctcatggt accacagggg ccgctgcctc tcacatcgtg cgggattagg
     3961 gtatactgcg gagcaccaac ttgtttgcag caacgcaggg gaaagcgcca tgcagagaca
     4021 cacccgcctg aatccttttt tcccggccat cctcctgttc gccatactga gcgtcagcat
     4081 cgcccgggcc ggcgaggaag cggcgcccgt ggagcttgtt tcggccacga agatctggga
     4141 tcagggaaat cacaacgcat tcacggacct gatccgcttt caggatcgct ggttctgctc
     4201 atttcgcgaa ggtgaggccc acgtcttcgg taaggacggc acgatccgtc tcatcacgtc
     4261 cccggacggc gagacctggg aatccgccgc gctcctgagc gaggaaggcg tcgacttgcg
     4321 cgaccccaaa ctctcgatca cccccgacgg acgcctcatg ctgctcgccg gcggctccgt
     4381 ttatcgcgga aaagagttta tcacccgcag accccgcgtc gccttcagca gtgacggccg
     4441 tacctggggc cccttgaccc cggtctgcga gcaaggtgac tggctctggc gcgtcacctg
     4501 gcataacgga acggcctacg gattctcccg cgctcccata ccccaggatg gcatcgaagc
     4561 caccagcgag gagtggcaac tcaccctcta ctccggcacg gacggcatcc attacacacc
     4621 tgtgtgcaaa cccaccgtgc ccggcaagcc caacgagacc accgtgcgat tcatggacga
     4681 cgacaccatg ctggccctgg tacgccggga aggcggtaac acccactgct ggatcggcgt
     4741 tgccaaaccg ccctacaccg actggtcgtg gcatgagacc ggctaccgca tcggcgggcc
     4801 caatttcatc atcctgcccg acgggtccat gtgggccggc gggcgcttcc atcccgatgg
     4861 tcccaaaacc atcctggccc gtttcggcgc ccagacttac gagcccgtgc tcactctgcc
     4921 cagcggcggc gacacctcct accccggttt cgtctggctc gaaggcctgc tgtggatgag
     4981 ctactactcc tcccacgaag ggaaaacgag catctatctc gcaaaaatac ggatcaaata
     5041 gccacaatag ccgcccccgg caaaacagtg cttccagcag actctgaatg cttccgtttg
     5101 gccttcagga tcaatggcct gactgatttc actgagccca gtggttatcc ggcctcatga
     5161 tttcgtgcct gcccgccctg cggacatttt cggcagactt gatcctcccg cacatcttgg
     5221 ggattatgcg acatgctggt gaaacctgga gtaaccacta tcgaggtgcg gctatgaaat
     5281 cacttgttgt ctccctggcc tttctgttca cccttccctg tctcgcaacc gccgtcgaat
     5341 tgcccggccc gaccagcgaa ggctatctcc tgccgaaccg ctggcgcatc actcccgtag
     5401 gcacggccat cgacaccaat gacctgctcc tgaacatcga aatagcgccg gacggcaaag
     5461 cggccatcgc ggtgcatggc ggatacaacg cccacggcct cgtggtgatc gatacggcca
     5521 gcggcacaac cgcccagacc atcccgttgc ccaccgcctg ggtgggcctg gtctggagtc
     5581 cggacggcgc cagactctac gtcgccggcg gcaataacaa gtcgtctaaa ggcatccgcg
     5641 cgcccatcta catctttggc tatcaggacg gacgcctcac cgatgaatcc atcggcactt
     5701 ttaccgagga aattgcgccc ggccgcatct tctggtcggg cctggcccac catccgtcca
     5761 aaaatatcct gtatgccgcg aaccgcacca acagttacgt cgtggtgttt gataccgcca
     5821 ccggcgagat cctccatcgc attccgacgg agctgaaccc ttacgaactg gccatcacgc
     5881 ccgacggcgc cacgctttac gcctccaact ggggcagcga cagcgtctcc atcatcgata
     5941 ccgaggccgg cgccgtgcgt cgaaccctcg ccgtgggcga caaccccaac gacctcgcga
     6001 tcgcccggga tggccgtctc ttcgtctgct gttccaacga caacacggtg acggtgatag
     6061 accccaggaa agagcgcccc acggagagca ttgtcacctc actgtatccc caggcgccgg
     6121 agggctcgac gcccaatgcg ctcgccatcg accacacgca gcatatcctg tatatcgcca
     6181 atgccgacaa caacaacgtc tgcgtcgtca gtgttgagaa gcccggcgaa tccgcggtgc
     6241 tcggattcat ccccgttggc tggtacccgt cgtccgtggc cgtcagcgcc gatggtgaga
     6301 ccctctacgt cggcaacagt aaaggaatcg ggcattcttc ggccaatcct cgcggtccgc
     6361 acagccccct tccgccgggc gagggcgccc agggtaccac caagacactg atgcgcggcg
     6421 ccatcagcat cattgacgtg ccccggcacc gccgcgatct gcgcaaactc accaagcagg
     6481 cctacgccaa ctgcccctac aacgacgaac tcctcgccac agcccggccc gaaacggagc
     6541 agaccgtcat cccgcgcgag gtcggaaagg gctcgaaaca gatcaaacac gtgatctaca
     6601 tcctcaaaga aaaccgcacg tacgaccagg tgctcggcga cctcccgcag gggaatggcg
     6661 acccccgcat caccattttc ggccaggaag tcacaccgaa ccagcacgcc atcgtggaga
     6721 cctttgtcct gcttgacaac ctgtactgcg acgccgaagt cagctccgat ggccaccagt
     6781 ggtctaatgc cgcgtacgcc accgacttca acgaaaaatc ctggcccgca agttacgccg
     6841 ggaaaagtga ttcgccgcgg tcgcctgcca tcatccctaa cgcgggctac ctctgggacc
     6901 agtgcgcgcg caaaggcctc acctaccgca gctacggtga attcgcgcgg cgcgtcagcg
     6961 acgacggcgt gatggaacca tccgatgccc gcttcaccgc actccatggc cacgttgcgc
     7021 ccaattacct caactggggg gcgcgcgatt acgaaaacgc cgccgaattc attcgggaat
     7081 ttgacgagta cgagaaaaat tacgacgatc ccaaccctga aaagcgcctg cccaacttca
     7141 tcgtcatggg ccttcccgag gaccacacca aaggcacccg gcccggcgtg cccacgccac
     7201 gcgctgcggt ggccagcaac gattacgccc tcggcctgat cgtcgaacgc gtgagtcaca
     7261 gcagatactg gccggaaatc gccttcttcg tgattgaaga cgacgcccag gacggggcgg
     7321 atcacgtcga cgcgcgccgt accacgggct acgtcatcag cccctacatc aaacgcggca
     7381 tcgtggacag cacgttctac accacctgct ccatgctccg caccatggag cttatcctcg
     7441 gcctgcagcc gatgagccag tacgacgccg cggccaatgc catgttcgcc tgcttcaccg
     7501 acacccccga cttgactccg tttaaacaca tcgaacccaa catcgatttg caggaactca
     7561 acatcaagac cgcctggggc gcggaggaat ccatgaaaat ggacttctcc gactacgacc
     7621 gcgccccgat gtttgagctc aatgagatca tctggaagag tgtgaaaggc ccggactctg
     7681 aaatgcccgt tcccgtgcac cggttcaata ccgccagcat tcaggaatga tgcgcggtgg
     7741 ccgcgatgcc gccgcgtttg cgcagtgaga tgacgactgc ggccaatggc ggccgatgaa
     7801 tgccgccagg ggctgatgca atgaaatctg ctcgaaacct gttggaagag gagaccgtca
     7861 tgcgctttct catcatcctt gcacttgcca cgaccattgc ggcgttttgc gcgcccgcag
     7921 aacctgtcca gctcgccacg gacggaaaac ccgccgtaag cgtcgttgtt gagcccaatg
     7981 ccggcagcct ggtctcaggc gcggcggacg atctccagtc ctacgtgaag gctctgtgcg
     8041 gcgtggagtt gccacggcac gacgacggag cggccgtcga cggaaaggcg atctacatcg
     8101 gccggtgcgc ccctgcgcca tccagcgcat ttccgccggc ggagtccaat ccggaaacct
     8161 atgccatcac cgcgcaggac ggcaatctct tcctgaccgg caaccatccc cctgcgacca
     8221 gcttcgcggt ctattctttt ctgcaggact atctgggcat ccgctggttt gctcccgggc
     8281 cgctctggga gtacgttccg gaagggcagc ccggagagct gacgctcgac gtcgcgaacg
     8341 gcgtcgttac ccccgactgg tcctgccggg tatggtccgg gcacgactat gctccatcct
     8401 ggaatttgtg gaaccgccgc agcaaaacca tctgtgattc tccggttcca ttccgcaaca
     8461 tgcagaattt tctgcacgtt atcttcgctc cggagaaata cgcggaaacc caccccgaat
     8521 actaccccct catcgacgga aaacgctgga tcccgcccgc cgattaccag aactggcggc
     8581 cctgtgaaag caatcccgac gtgctgcgca tcaccgtcga ggccgctcgc gaatttctcg
     8641 acgcgcaccc cgagcacaac gcgttctcgc tcgccatgga cgacatctac cgcctctgca
     8701 gctgcgacaa ctgccgagcc cgggacgccc gcccggatga ctatgaaaac aagcggttct
     8761 ccgaccggca ctacaaattt gtgaacgccg tcgcgcgcga actcgccaaa acccatcccg
     8821 acaaattcgt tggaaccctc tgctaccaca tcgcccgtga acttcccgaa accgtggagc
     8881 agctcgagcc caacgtattc atcagcatga cccagcgggt cggcgaatgg tggcgtcctc
     8941 agcggcacgc cgacgacatc gccctgacgc atgcctggcg caaacgctgc tctcacatga
     9001 gccgttacgg ctatatcggg ctcggtttcc tgaccccgcg cgtcttccct cacgccatgg
     9061 cggagggtat gaaactcgac aagagcctcg ggtttgaggc cgtct
//
LOCUS       DAOPBQ010000067         8968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7315_length_8968_cov_3.852575, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000067 DAOPBQ010000000
VERSION     DAOPBQ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7315_length_8968_cov_3.852575"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1633
                     /locus_tag="PKW60_03555"
     CDS             <1..1633
                     /locus_tag="PKW60_03555"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPJ98537.1"
                     /translation="ELGCRWVRPGEDGAYVPQLERLPDVRISETPSYRHRGVCIEGAV
                     SYEHVRDMVDWLPKLGFNAYFIQFREAHTFFDRWYRHTGNPNEAGEALSIEKAREYTE
                     MLETEIKRRGLLYHKVGHGWTCEPFGIAGLGWEEERRSAPPEVAKYLAEVRGKRDFWK
                     GVALNTNLCYSNPEVRRIIVDAVVAYAREHPAIDYIHFWLGDGTNNNCECAQCVRARP
                     SDFYVMMLNELDQRLTKAGLATRIVFLIYVDLLWPPLEQRIKNSDRFVLMFAPIVRTY
                     SETFEASGPLPELPPFERNRLVFPKSVEANVAFLKAWQAFFDGDSFDFDYHLMWDHSN
                     DPGHMQIARTISADMKGLAKIGLNGYVSCQIQRIFLPTGLAMTVMGRMLWDAKADFDA
                     VADDHFRSAFGADGPACRDYLERLSELFDPVYIRGEKEWLNAEQAQRFARAPDVIQGF
                     MLTIAANLRSGDSCHRASWRYLKHHAELVLGLARAFHAKALGMDEAARKAWETTKKLA
                     WDKEPDLHPVLDTWLFAMTALEAKFVPGKGDGAPG"
     gene            1884..2354
                     /gene="msrA"
                     /locus_tag="PKW60_03560"
     CDS             1884..2354
                     /gene="msrA"
                     /locus_tag="PKW60_03560"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPJ98538.1"
                     /translation="METATFAAGCFWHVEEAFRLVEGVKATSVGYTGGHTEEPTYQEV
                     CTGTTGHAEAVEVVYDPELVSYEELLALFWKVHNPTQKDRQGPDIGHQYRSAIFCHTP
                     EQEKAARASMKALDESGRYRQPIVTEIVKAGTFWRAEEYHQQYFLKHGLSHCPR"
     gene            2498..2992
                     /locus_tag="PKW60_03565"
     CDS             2498..2992
                     /locus_tag="PKW60_03565"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPJ98539.1"
                     /translation="MRIAEAMAQEMAQEARGTRKLLERLPVDQFGWTPHEKSMTLGRL
                     ASHVAECFGYTLPIITTDSMQLDPDNYKPAVAESVAEILQVFDKDLEQALDALDGVPD
                     KHMMAGWKMLMGEQVILDLPRYAAMRGVVLNHLIHHRGQLTVYARQCNVPLPALYGPS
                     ADEA"
     gene            complement(3077..4258)
                     /locus_tag="PKW60_03570"
     CDS             complement(3077..4258)
                     /locus_tag="PKW60_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98540.1"
                     /translation="MQKKTVRAGIVGAGFAARFHYDAMRRVYGAHVDIKGVYARRAEP
                     AQAFAGERGIHCYESLDALMDDVDVVHCCVTVDAHEEITIAALERDKHVIVEKPLTGY
                     LGDGTDEFHGDLFPKEDALKSGLASVDRMLAAEAKSRGHILYAENWVYAPAIQREREI
                     IEKTGAQILWMHGEEAHSGSHNPTYAYWKNSGGGVLIGKGCHPLSAALYLKRVEGRAR
                     DGNPIRPKTVTARAHALTRMKTFRDAGHIRCDYHDIDDFSMMHITFEDGTVATVFASD
                     IILGGIHNWLEVAANNHRTICNINPNTAMQVYNPKEANFADIYTVEKIETKQGWTNIP
                     PDEDWFTGYPQEIEAFYRTAAYGEPVESDSGLAASTIATIYSAYLSNERTGAEVEIKA
                     Y"
     gene            4439..5722
                     /locus_tag="PKW60_03575"
     CDS             4439..5722
                     /locus_tag="PKW60_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPJ98541.1"
                     /translation="MRNALWFIIGGIVLALAFAVGSPYLAYAAYAFLLLVAIAHFSSW
                     AWLSGLDCQRTIDRATVHQGESITIDVVVENRRGWPIPWLYVEDLHPPDFPRDGDNAR
                     LGVLMPGRSMRFHYRLTCPRRGYHRIGPLLMESGDLFGLQRRFVTGKQQDYVSVLPTV
                     AYIETFTITARRPQGPVRISNRIYEDPSRIAGVREYQRGDPLNRIHWKASARTGDLFT
                     KQAEPSNVLGATLVLDLHKDAYAGANAERRMELAITTVASLAYLLQLSGEQLGMITNA
                     RDAAEVARYEVAGTEALSRQDAESGVAGEERADTLRPLEVPTRRSPVQALHIIENLAR
                     VLPTDGLDVGALLMAEFRKQPRDATLLPVVPQVTPEFARVLAEMKLSGFSVTVFMIDN
                     YHGYEEASVLLAADGIDVLHIETERDLHEISPARI"
     gene            5697..7451
                     /locus_tag="PKW60_03580"
     CDS             5697..7451
                     /locus_tag="PKW60_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HPJ98542.1"
                     /translation="MKSHRPESDGTARPPEQPEATDFASLMAPPVAQRQQAKAPAATD
                     FASLKRSLSQSPVKRWQKHTATDLLIDGLIPLLILLMVTSFVMFLLDIRYVYTEVSDM
                     NIRVFALCLVLGVVALNRLVARDGTEESLVYFVLLAAATGFYTFVTTGAYDVGSVAGE
                     FMNHPYVATGFNMTLVILLWWATNRLMHECCVDENLTAGDIGILTGTARRIVAATRAR
                     PRMPKSSSSQSMWLDLDAYDPSEARPASPAAPKRTERPVKRLQKRHPGISIFYFTVPV
                     VFVFALGPRIVGHGGGRLIFSGWVLLVVYLLSALSLLMLTSLGGLRAYFRNRRVTIPG
                     GIGPFWVGLGAVLVLAVLVGAAALPKPAWPPLLHVGEHQYDPWNRGSTFRLSLVEVTA
                     EEQARLTALTQYVGYGVLAVLGLFALYGLLRALGALAFALARQRRRLPRWLTNFFDAL
                     DRLLLRLARLPSLPAFKRRIRVSRSLSQSIRYSNPLAQGEPRGRAGIAQAVATSYDAL
                     CALASDMGLPRREEQTPYEFIQSFPEGLEGLRAEAMSLTQLYVRSQYSDLDLEPRTLD
                     ALRRFWLAFEPIRRQYVR"
     gene            7571..8941
                     /locus_tag="PKW60_03585"
     CDS             7571..8941
                     /locus_tag="PKW60_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98543.1"
                     /translation="MNRRATTRRNFLKMSAALGAAPWIVPSSVFGAKAPSNRVNVAHL
                     GMGGRANSLVGMFVRHDDVQILAICDCFAHRREEMRDKLNAKYGGDVVKAYADFREVL
                     SRDDVDAVVIATPDHWHVPLAIAAARTGKDMYVEKPLGVCMSWAERLRSELQAAKRVF
                     QYGTQQRSNTAFRTACELVRNGYIGEIQRVEAWCPDMSTQFDGFNVKPYGSTEPAELP
                     EGFDYDMWLGPAPEKPYTVDRCTPLGAYHIYDYALGFIAGWGAHPLDIAQWGLGRDHT
                     SPVYYTGTGKIPAGGLCDSIESWDVHCEYEDGLPIRFMGSRVAEPIVSTYRKWGDHGT
                     TFFGSDGWVSVDRSGMITNPPALADVQLKADDLRLKVSEGQERDFIDCVKSREETISP
                     IESAVRSDAISHLSDLAIRVDRPIRWDPRHERVVDDPGAAQRLHRPLRQPWNLDKPMV
                     TDGWEG"
BASE COUNT         1638 a         2657 c         2903 g         1770 t
ORIGIN      
        1 ggagctgggc tgccggtggg tgcgcccggg cgaagatggg gcatatgtgc cgcagctgga
       61 acgcctgccg gacgttcgaa tctctgaaac gccgtcttac cgccaccgcg gggtgtgcat
      121 tgaaggggcg gtgagctacg aacacgtgcg ggatatggtg gattggctcc cgaaactggg
      181 atttaacgcg tacttcatcc agtttcgcga ggcgcacaca tttttcgacc gctggtaccg
      241 gcacacgggc aatccaaacg aagcgggaga agccctgtcg atcgaaaagg cgcgcgaata
      301 cacggagatg ctggagacgg agatcaaacg ccgcgggctg ctctatcaca aagtcggcca
      361 cggatggacc tgtgaaccgt tcgggatagc cggcctgggc tgggaagagg agaggcgcag
      421 cgccccgcct gaggtggcaa aatacctggc cgaagtgcgc ggtaaacggg atttctggaa
      481 aggggtggcg ctcaatacca acctctgtta ttcaaacccc gaggtacgcc gcatcatcgt
      541 ggacgccgtg gtcgcatatg cgcgcgaaca tccggctatc gactacattc acttctggct
      601 gggcgacggt accaataaca actgcgaatg cgcgcaatgc gtgcgggccc ggccgtccga
      661 tttctatgtc atgatgttga acgagctgga tcagcgcctg acgaaggcgg gcctggccac
      721 gcgcattgtg tttctcatct atgtagacct gttgtggccg ccgctggaac agaggatcaa
      781 gaattcggac cggttcgtgc tgatgtttgc gcccattgtg cgcacttaca gcgagacctt
      841 cgaagccagc ggtccgctgc cggagcttcc tccctttgaa cgcaacagac tggtatttcc
      901 caagagtgtc gaggccaacg tggctttttt gaaagcctgg caggcgttct tcgatggcga
      961 cagctttgat tttgattacc acctcatgtg ggatcactcg aacgatcccg gacacatgca
     1021 gatcgcacgc accatttccg ccgacatgaa gggcctcgcc aaaatcggat tgaacggcta
     1081 cgtgagctgc cagatccagc gcatcttttt gcccaccggc ctcgcgatga cggttatggg
     1141 gcggatgttg tgggacgcga aggctgattt cgacgcagtg gcggacgacc atttccgaag
     1201 cgccttcggg gccgatggtc ccgcatgccg cgactatcta gaacggttgt ctgagctttt
     1261 cgacccggtg tatatccgcg gggagaagga gtggctgaac gccgagcagg cgcagcgttt
     1321 cgcccgggcc ccggatgtga ttcaggggtt tatgctcacg atcgcggcaa acctccggtc
     1381 gggcgattca tgccaccggg catcctggcg ttacctgaaa caccacgcag aactggtgct
     1441 gggactggcc cgggcgtttc acgccaaggc gctgggaatg gacgaagccg cccggaaagc
     1501 ctgggaaacg acaaaaaaac tcgcgtggga caaagaacct gacctgcatc cggtcctaga
     1561 cacgtggctg ttcgccatga ccgcgctcga ggcgaagttt gttcccggca aaggcgacgg
     1621 cgcgccggga taaattccgc ttgcccccgt gtttctcccg ggtagaaccc ggctcggtgg
     1681 gcatcggcct tcctggcggc ccgtgcggga ccaaatgccg ggcgctgatt gtttacgaag
     1741 gagccgtgat acgcatgaaa tgggccgcac tggccgcgct tgtagtgata gtactctcgc
     1801 tccatctcgc tgggcaggcg gaggtggaga cggcacaccg gcgcgtgaca ggcgatacaa
     1861 caccactcag aggagtcgaa ggcatggaga cggcgacctt tgctgcgggc tgcttctggc
     1921 acgttgagga ggccttccgc ctcgttgagg gggtgaaggc cacttcagtg ggctataccg
     1981 gcgggcacac ggaagaaccc acgtaccagg aggtctgcac gggaactacc gggcacgccg
     2041 aggctgtcga ggtggtctac gatccggagc tggtcagtta cgaagagttg cttgcgctgt
     2101 tctggaaagt gcacaacccc acccagaaag atcgccaggg ccccgatatc ggccaccaat
     2161 accggtccgc aatcttctgc cacacgccgg agcaggagaa agccgcccgg gcgtcgatga
     2221 aggcactgga cgaatccggc cgataccgtc agccaattgt gacggagatt gtcaaagcgg
     2281 ggacattctg gcgcgcggag gaataccacc agcagtactt tctgaaacac gggctgtccc
     2341 attgtccccg ctgacgcccg ctgaatccct gtgatcccca gggcagccgt tggaagcccg
     2401 gcttatgggc acggtccggt cgtttttgca cagtagaagt agagtccaca ggttgcgatg
     2461 gcggagctgc gggtctcgcg gcgaagggag ggacatcatg cgtattgcgg aagccatggc
     2521 gcaggaaatg gcccaggagg ccaggggtac gcgcaaactg ctcgaacgtc tgcccgtaga
     2581 ccagttcggc tggacgcctc acgaaaaatc gatgaccctg gggcggctgg cctcccacgt
     2641 ggcggaatgc tttggctaca ccctgccgat catcaccacc gactccatgc agttggatcc
     2701 cgacaactac aagccggcgg ttgcggagag tgttgctgaa atccttcaag tctttgacaa
     2761 agatctcgaa caggccctgg acgcgctgga cggcgtgccc gacaagcata tgatggcggg
     2821 atggaagatg ttgatgggtg agcaggtcat cctcgacctg ccgcgctatg ctgcgatgcg
     2881 cggagtcgtc ctgaatcacc tcattcatca tcgcggccag ctgacggtgt acgcgcgcca
     2941 gtgcaacgtg ccgcttccgg cgctgtacgg gccgagcgcg gatgaagcat aggcgttttc
     3001 actcgctttg acgagcgcct gcagcgcgtg cgtgcgcgcc atcgaccggc gatgtccgcc
     3061 gtgcagggcg ggtgcgtcaa tacgccttga tctcgacctc cgcgccggtg cgctcgttcg
     3121 aaaggtaggc gctgtagatg gtcgcaatcg tgctggcggc gagcccgctg tcgctctcga
     3181 caggctcgcc gtacgccgcg gtgcggtaaa acgcctcaat ctcctgcgga tatccggtaa
     3241 accagtcttc atcaggcggg atattcgtcc agccctgctt ggtttcgatc ttctcgacgg
     3301 tatagatatc ggcgaaattg gcttccttcg gattgtagac ctgcatggcg gtattggggt
     3361 tgatgttgca gatggtgcgg tggttgttgg ccgctacttc cagccagttg tggatgccgc
     3421 ccaggataat gtcggacgcg aaaacggtgg cgacggtgcc gtcttcgaag gtgatgtgca
     3481 tcatcgaaaa gtcgtcgatg tcatggtaat cgcagcggat gtgtccggca tcgcgaaagg
     3541 tcttcattcg agtaagggcg tgagcccggg cggtgacggt tttcgggcgg atggggttgc
     3601 cgtcgcgtgc gcggccttcg acacgtttga ggtagagcgc cgcgctgagc gggtggcagc
     3661 cttttccgat gagcacgccg ccgccggagt tcttccagta ggcgtaggtg gggttgtgtg
     3721 acccggaatg ggcctcttcc ccgtgcatcc acaggatctg cgcgccggtc ttttcgatga
     3781 tctcgcgttc gcgctggatg gcgggggcat atacccagtt ttccgcgtag aggatatggc
     3841 cccggctctt cgcttccgcg gcaagcatgc ggtccacact cgccagacca gacttcagcg
     3901 catcttcctt ggggaaaagg tcgccgtgga actcgtccgt gccgtcgccc aggtagccgg
     3961 tgagcggttt ctcgacgatc acatgtttgt cgcgctccag ggcggcgatg gtgatctcct
     4021 cgtgcgcgtc gacggtcaca cagcagtgga cgacatccac atcgtccata agcgcatcca
     4081 gggattcgta acagtgaatg ccccgttcgc ccgcaaatgc ctgcgccggt tccgcgcggc
     4141 gcgcatagac acccttgatg tcgacgtggg cgccataaac cctccgcatc gcgtcgtaat
     4201 gaaaccgggc ggcaaatccc gctccgacaa tccccgcacg aaccgtcttc ttttgcatcg
     4261 cagctactcc attgtgatta tttatcccct gccggctggt gcaggccgga tgagtgcgtg
     4321 ccggaggcag tctaggttat ggccgtgagc ggagtcaacg gacagcgtgg aaaacaggtg
     4381 ctttttccgc ctggttgagt ttttctctcc gttccgctca tggcatgata gacaggatat
     4441 gcgaaacgcg ctctggttca tcatcggcgg catcgtgctg gctcttgcgt ttgcagtggg
     4501 gagcccgtat ctcgcgtatg ccgcgtacgc cttcctgctc ctcgtggcga tcgcacactt
     4561 cagctcgtgg gcctggctga gcgggctgga ttgccagcgc accatcgatc gcgccacggt
     4621 gcaccagggc gagagcatca ccatcgatgt ggtggttgag aaccgccgtg gatggcccat
     4681 tccctggctc tatgtcgagg acctgcaccc gcctgatttt ccacgcgacg gcgacaatgc
     4741 ccggctgggc gtgctcatgc cgggccgatc catgcgcttc cactaccggc tgacctgtcc
     4801 acggcgcggt tatcatcgga tcggcccgct gctgatggaa tcaggcgacc tgttcgggct
     4861 gcagcgccgc ttcgtcacgg gaaaacagca ggattacgtc tcggtgctgc cgaccgtggc
     4921 ctatatcgag acgtttacga tcacggcgcg gcgtccgcag ggcccggtgc gcatcagcaa
     4981 ccgcatttat gaggatccgt cccgtattgc aggcgtccgt gaataccagc ggggcgaccc
     5041 tctgaaccgc atccactgga aagcgtcggc ccgcaccggc gacctgttta cgaaacaggc
     5101 cgaaccgtcg aacgtgctcg gagcgaccct ggtgctggac ttgcacaaag acgcgtatgc
     5161 gggcgcaaat gcggaacgac gcatggaact tgcgatcacc accgtggcgt cactggcgta
     5221 cttgctccag ctttcgggcg aacagctggg tatgatcacc aatgcgcgcg atgctgccga
     5281 ggtcgcgcgt tatgaggtcg cgggcaccga agcgctgtcg cggcaggatg ccgaatcggg
     5341 ggtggcgggc gaggagcgcg cggacacgct ccggccgctc gaagtgccga cgcggcggag
     5401 tcccgtgcag gcgctgcaca tcatcgaaaa tctggcacgg gtgttgccga ccgatgggct
     5461 cgacgtgggc gcgcttttga tggcggaatt ccgcaaacag ccgcgggacg ccaccctgct
     5521 cccggtggtg ccgcaggtga cgcctgagtt cgcgcgtgtg ctcgccgaga tgaagctgtc
     5581 agggttttcg gtgaccgtgt tcatgatcga caactaccac gggtacgagg aggcgtcggt
     5641 gctgctggcc gcggacggga tcgacgtgct gcacatcgaa acggaacggg atctccatga
     5701 aatctcaccg gccagaatct gacggaacgg cccggccgcc ggaacagccc gaggcgactg
     5761 acttcgcgtc gctgatggcg ccgccggttg cacagcgcca gcaggcaaag gcgcccgctg
     5821 ccacggattt tgcgtcgctc aaacgatccc tgtctcaaag cccggtgaaa cgctggcaaa
     5881 aacacacggc gacggatttg ctgatcgatg ggctcatccc cctgctgatt ctgctcatgg
     5941 tgacttcgtt tgtgatgttt ctgctggata tccggtacgt ctacaccgaa gtgagcgaca
     6001 tgaacattcg agtgttcgcc ttatgtctgg tgcttggcgt ggtggccttg aaccggctcg
     6061 tggcccgcga tggcacggag gagtcactgg tctattttgt gctcctcgcg gccgccacgg
     6121 gtttctacac cttcgttacg acgggagcct acgacgtggg atccgtcgcg ggggaattca
     6181 tgaatcaccc gtatgtggcc acgggcttca acatgacgct ggtcattctc ttgtggtggg
     6241 cgaccaaccg cctgatgcac gagtgttgcg tggacgagaa cctgaccgcg ggcgatatcg
     6301 gcattctcac cggcacggcc cggcgtatcg ttgcggcgac ccgggccaga ccgcggatgc
     6361 cgaaatcatc ctccagccag agcatgtggc tcgatctcga tgcctacgat ccgagcgagg
     6421 cgcgtccggc ttcgccggcg gcccccaaac ggacggaaag gccggtgaag cgtcttcaga
     6481 aacggcaccc cgggatttcg atattttatt tcacggtgcc ggtcgtattt gtttttgcgc
     6541 tcggcccccg cattgtcggt catggcgggg gacggctgat tttcagcggc tgggtgctcc
     6601 tggtggtcta cctgctgtcg gcgttgtcac tgctgatgct gacgagcctc ggcgggcttc
     6661 gcgcctattt ccgcaaccgg cgagtaacga tacccggggg cattgggccg ttctgggtgg
     6721 gcctgggtgc ggtactggtc ctggcggtgc tggtgggcgc ggccgcattg cccaagccgg
     6781 cctggccgcc gctgctgcat gtcggggagc atcagtacga cccgtggaac aggggctcaa
     6841 cgttccggct ctcgctcgtt gaagtcacgg cggaggaaca agcccggttg accgcactga
     6901 cgcagtacgt gggctacggt gttctggcgg tgctgggcct gttcgcgcta tatggactcc
     6961 tgcgggccct gggcgcgctc gcgtttgcgc tggcacggca acgccgccgg cttccccgct
     7021 ggctgacgaa cttctttgac gcgctcgatc gcttgctgct ccggttggcg cgccttccca
     7081 gcctgcccgc gttcaagcgg cgtatacgtg tcagccggtc gctgtcgcag tccatccgtt
     7141 acagcaatcc cctggcgcag ggtgagccgc gaggacgtgc cggcatcgcc caggcggtcg
     7201 caaccagcta cgacgccctc tgcgcgcttg ccagtgacat gggccttccg cgccgcgaag
     7261 aacagacgcc gtacgagttc attcagtcct ttcccgaggg gttggaaggc ttgcgcgctg
     7321 aggccatgag cctgacccag ctctacgtac gctcgcagta ttcggatctg gacctcgagc
     7381 cccgcaccct cgacgccttg cgccggttct ggctcgcgtt cgaaccgatc cgtcgccagt
     7441 acgtccgctg actcgtggag tcattgcgca ttcgactctg ccgatccgga aaatgttttt
     7501 ccgtggtatg atgcgggcag gctccgaaag tgtgcgattg gggagaagct gatttccgag
     7561 gagaatctgc atgaatcgtc gagccacgac ccgccgcaat tttctcaaga tgtccgccgc
     7621 gctcggagca gcgccctgga ttgtcccgtc gagcgtgttt ggggcaaagg cgccgagtaa
     7681 ccgggtaaat gtagcgcacc tgggcatggg gggccgcgcc aattcactgg tcgggatgtt
     7741 tgtccgacat gacgacgtgc agattctggc tatttgcgac tgtttcgcac accgtcgcga
     7801 agaaatgcgc gacaagctca acgccaagta cggcggtgat gtggtgaaag cgtacgcgga
     7861 ttttcgcgaa gtgctgagtc gcgacgacgt cgacgccgtt gtgatcgcga cgcctgacca
     7921 ctggcatgtg ccgctggcaa tcgccgccgc ccggaccggc aaagacatgt acgtggagaa
     7981 gccgctcggg gtgtgcatgt cgtgggcgga gcggctgcgc agcgagctgc aggctgcaaa
     8041 acgcgtgttt cagtacggca cgcagcagcg ttcgaacacg gcatttcgca ctgcgtgtga
     8101 actggtgcgc aacggataca tcggcgagat tcagcgcgtc gaggcgtggt gccctgacat
     8161 gagcacgcag tttgatggat tcaacgtgaa accctacggg tcgacggagc ccgcggagct
     8221 tccagagggt tttgattacg acatgtggct cgggcccgca ccggagaagc cctacacggt
     8281 cgatcgctgc actccgcttg gcgcgtacca catttacgat tacgcgctgg gattcatcgc
     8341 gggctggggc gcccatccac tggatatcgc gcagtggggg ctgggccgcg atcacaccag
     8401 cccggtgtat tacaccggga ccggcaaaat ccccgcgggc ggcctctgcg acagtatcga
     8461 gtcgtgggat gtccattgtg agtacgagga cggtctgccg atccggttca tgggaagccg
     8521 cgtcgccgag ccgatcgtga gcacgtaccg caaatggggc gaccacggga cgacgttttt
     8581 cggcagcgac ggctgggtga gcgttgatcg gtccgggatg atcaccaacc cgcccgcgct
     8641 ggcggatgtt caactgaaag ccgacgacct gcgccttaag gtgtctgagg gccaggaacg
     8701 cgattttatc gactgtgtaa aatcgcgtga agagacaatc agcccgatcg aatcggccgt
     8761 tcgctctgac gccatcagtc acctcagcga cctggccatc cgagtcgacc gcccgatccg
     8821 gtgggacccc cggcacgaac gcgtcgtcga cgatcccggc gcggcgcaac gcctgcaccg
     8881 cccgctgcgc caaccctgga atctcgacaa acccatggtc accgacggct gggaaggctg
     8941 attggcgagt aaagagagtt gcctgtgc
//
LOCUS       DAOPBQ010000068         8952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7334_length_8952_cov_4.504552, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000068 DAOPBQ010000000
VERSION     DAOPBQ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7334_length_8952_cov_4.504552"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..1729)
                     /gene="flgK"
                     /locus_tag="PKW60_03590"
     CDS             complement(38..1729)
                     /gene="flgK"
                     /locus_tag="PKW60_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPJ98544.1"
                     /translation="MGTLFSALDVARSGLTAAQIQIDTAAHNIANVNTEGYSRQRAEL
                     LTRLPTSFPYGQLGRGVQVNNIVRIHDSFIDQAYRNQLPGQTAAELTAGYYALIDGIF
                     LEPGENGFGARIGQFFDALSDFANNVESSSVREALVAETRQLSLSFNDVALRFNTLRT
                     NANEEVRSLVPQINSLAERLADINQQIVLSEANLTSANDLRDHRDRLLDELAGLVNIT
                     TVENGNLVSVFIGNDALVEGVLWNRLEAVRDPSLDPRRADLLRVRFAGTGLATDVRDG
                     ELHAALAVRDEIIPEYQRRLDTLANALIRAINTIQTQASGLSNLSGMITSSNAVADPS
                     AALVGGSLPFAVEPGTFDVVVYDATGTPTVTTVTITAATTLNDLAASLNSIANFSAAV
                     VDDRIEFGTSGGYTFGFANDSTGVLAALGTHGLFTGHNAASIGLNPLIDDDPGLIASG
                     YSLDPLATGDNRAALAMAALRTALILDGGASSFSDYYEATIAQLGVESRANQERLAIQ
                     QQFVSDFQQRRQQISGVSLDEEISLMIQYQRAFEGSARVITVTDRMLEALLTMAL"
     gene            complement(1940..2875)
                     /locus_tag="PKW60_03595"
     CDS             complement(1940..2875)
                     /locus_tag="PKW60_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPJ98545.1"
                     /translation="MLFTCLGVFLIAAGAAHASESVSEVKTIYVTSTGDATEQPSLFI
                     EAQGAGPRPLLVFLHTWSSGYDLDTQPWRDEAAQRNWHFLQPHFRGPNNTPDACASPL
                     ARQDILDAVGYVLQRHAVDPARVYLAGASGGGHMTLVMAAHAPERWAAASAWCAITDL
                     AAWHRETLEMGNKYARDIELCVGGAPGSSQAVDEQLRYRSPVHQLHRARGLPLDLATG
                     IHDGHTGSVPVHHTLDAFNVLANAHGAPLVNAAEMDALAREESLPSREEQDPAFERTI
                     YLRRYAGSCRVTIFEGGHEGLPKAALAFLARYVRE"
     gene            complement(2896..3552)
                     /gene="eda"
                     /locus_tag="PKW60_03600"
     CDS             complement(2896..3552)
                     /gene="eda"
                     /locus_tag="PKW60_03600"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPJ98546.1"
                     /translation="MNKFDAIHYLLKKCIIAVVRADRGGDDLVKVVEAVAAGGVHCIE
                     VTMTTPGALDCLKTASEKLRDADVLLGVGTVLDPETCRMAILAGARYVVTPTLSLPTI
                     QMARRYGRPILCGAYTPTEILTAWENGADFVKVFPAQVGGPGYIKALKGPLPQVPLVP
                     TGGVELENVGAFLSAGAAALAVGGNLVSNKQVLAQDFAGITANARAFADAVNAARGEQ
                     "
     gene            3807..4568
                     /locus_tag="PKW60_03605"
     CDS             3807..4568
                     /locus_tag="PKW60_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPJ98547.1"
                     /translation="MATIRIVGVQMEVSPKLDVNLPKMIDHIMNADGDFVLFPEMSLT
                     GYHGNFNDKAVRAAWEQIAKACRRKYITALIGTGRKRDDDVHIQTRIYTDAGELLGTH
                     EKLVPTRADREFCKPGEKLRIFHHRGLSFGCTICNDLWVTPGSGPYPDPRLTYQLGQK
                     GAQVIFHSANSGADPRFRTFHESNLQLRAIESQVYIATANAASPDGPVNSPSGIMSPE
                     GQWLVQCPLEGEQTYSHDIDVEIDVGAEEEGEAVK"
     gene            4693..5931
                     /locus_tag="PKW60_03610"
     CDS             4693..5931
                     /locus_tag="PKW60_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98548.1"
                     /translation="MTTGTHSRRRFLARSTALGAATALAARGARASEKSRPEPKTPAE
                     PLRCAVIGVGKRGGALLQTLLELDSVQVVALADTYDVWRNRALAWCKQKNGETSDYVE
                     YEHLLKKEKLDAVFIATPPHTLAPAALEALGRHCAVYIERPMALTWEAAKAVRAKARE
                     TGAVVQVGSHYRSTDLYLRAREILASGELGKLAMVQVNHYDMGQALDPESLPIEATEE
                     SLHWPLFLGDTREYPFDPLRYFHWVHFEEYSGGLNGGLLADHLDTCHFLADAGMPARV
                     MSAGSLAKFNDGRSCPDTVNTIVEYPNGLQFTYTATLTNERFGVEERYIGTGGALYIR
                     NMQEMTIYLEDIEENLKSNGPNTRAHIEDFLDAVRSGGTPRATVDDGFRAAAACHMAV
                     LSDQTGKTIAWDAGEEAVAV"
     gene            complement(5993..7039)
                     /locus_tag="PKW60_03615"
     CDS             complement(5993..7039)
                     /locus_tag="PKW60_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98549.1"
                     /translation="MIARRLLTGVLAVAIGFACCVAAQRQMDASRKTGFDEELLYLPN
                     EKLLNHFTGGLSSVVADLLWLQCIQYTAEEFKGEHKYVWLNHMIDTITRLDPYFVDVY
                     RYGGVFLAALKADSDASIDLMKRGMIHNPGAWVLPYEMAMVYLLNRRENPGSPAQAAQ
                     FLQLAVATGTAPQYVIDLAANIARQHDLDEFERAMWEDMARNSSEKMLRELAQRKLHE
                     LAIADVCRQLGKAVQLYEERHHRLPDSLNDLVAGGIIERLPDDPLGGRFFLAGREVLN
                     TTLQEEKLERRINRIRNGVNRYQARTGAWPPSLEAAVEGGDLVFVPPHPYPGRAWRYD
                     PATGEVEGGSAPEL"
     gene            complement(7036..7797)
                     /locus_tag="PKW60_03620"
     CDS             complement(7036..7797)
                     /locus_tag="PKW60_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ98550.1"
                     /translation="MRVLAVARNTFREAVRDKVLYVLLLFAAAAIFGSKALGWISIGQ
                     DIKIVKDLSLAAVSVFGVLIAIFVGTNLVYKEIDKRTIYTILCRPMHRWEFILGKYLG
                     LAALLALVTAVMVLVAAVYVVVLGGSLDATFFLAGLLIGWQLLLITALAVLLSALTSP
                     ILGAIIVFSTYVVGHATTIFTELPPHFDGTATKRVLQAVYYIIPNFSNFDIRAEAANG
                     VPVAPGYILWAMAYGAVYTAVLLIIASLAFQDKDV"
     gene            complement(7794..8720)
                     /locus_tag="PKW60_03625"
     CDS             complement(7794..8720)
                     /locus_tag="PKW60_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98551.1"
                     /translation="MDAIATRNLTKIYRGHIGRGAVKSLDNLSLAVAPNEVFGFLGRN
                     GAGKTTAIKILCSLLRATEGEAFVMGQDTRRPATRKHIGYLPENPYFYEYLTPRETLD
                     FYGELHGLTRAERAAEWEQLADLLDLRGIAGQRVRGFSKGMRQRLGFAVALVGDPEVL
                     ILDEPMSGLDPLGRRMIRELILLFHERGKTIFFSSHVLGDVEQICSRVGILVQGRLTT
                     QGRLDELLNRRVNRVEIIAADLNEAAATVLAARSDGIRESEQGRHFFVKDMEAANALA
                     ADVLQSGGTLAEFTPIRESLEEYFVREQETVS"
BASE COUNT         1730 a         2762 c         2829 g         1631 t
ORIGIN      
        1 cttctagagg gatatttcca ggactgtcgc ggccggctta caacgccatc gtgagcaggg
       61 cttcgagcat gcggtcggtg acggtgatga cgcgtgcaga gccctcgaac gcgcgctggt
      121 actggatcat gagcgagatt tcttcgtcga gggacacgcc cgagatctgc tggcggcgct
      181 gctgaaaatc gctgacaaat tgctgctgta tcgcgaggcg ttcctggttc gcgcggctct
      241 cgacgcccag ctgggcgatg gtggcctcat aatagtcact gaacgaactg gcgccgccgt
      301 cgaggatcaa ggccgtacgg agcgccgcca tcgcgagcgc ggcgcggttg tcgccggtgg
      361 cgagcggatc gaggctgtat ccggacgcga tgaggccggg atcgtcatcg atgagcggat
      421 tcagcccgat cgacgcggcg ttatgcccgg tgaacagccc atgagtgccc aacgccgcca
      481 ggacgcccgt gctgtcgttg gcgaacccga aggtgtaacc gccagacgtg ccgaattcga
      541 tgcggtcgtc cactacggcg gcggagaaat tggcgatgct gttgagcgac gcggcgaggt
      601 cgttcagggt ggtagcggct gtgatggtga cggtggtgac ggtcggcgtg cctgtggcat
      661 cgtagaccac cacatcgaag gtaccgggct cgaccgcaaa aggaagcgag ccgcccacga
      721 gcgcggcgga cggatcggcg accgcgttgc tgcttgtgat catgcccgac agattgctga
      781 ggccgctggc ctgggtttga atcgtgttga tggcgcgaat cagcgcattg gccagggtat
      841 cgagccgccg ctggtattcc gggatgattt cgtcgcgcac ggccagggca gcgtgaagtt
      901 cgccgtcccg gacatccgtg gccagacccg taccggcgaa ccgcacccga agcagatcgg
      961 cgcggcgggg gtccagggac ggatcgcgca cggcttcgag ccggttccag agcacgcctt
     1021 caacgagcgc gtcgttgccg atgaacacgc tgaccaggtt gccgttctcg accgtcgtga
     1081 tgttgaccag gccggcgagt tcatccagga ggcggtcgcg atggtcgcgc aggtcattgg
     1141 cgctggtgag attggcttcg gaaaggacga tctgctggtt gatatcagcc aggcgttccg
     1201 cgagcgagtt gatctgcggc accaggctcc gcacctcctc gttggcgttg gtacgcaggg
     1261 tattgaaccg cagggctacg tcattgaagc tcaggctcag ctgacgggtt tccgccacga
     1321 gcgcttcgcg caccgacgag gattcgacgt tgttggcgaa atcgctgagg gcatcaaaaa
     1381 actggccgat ccgcgcaccg aacccgtttt cgccgggttc gagaaagatg ccgtcgatca
     1441 gggcataata ccccgcggtc agctccgcag ccgtctggcc gggcaattgg ttccggtagg
     1501 cctggtcgat aaacgagtcg tggatgcgga caatgttgtt gacctggacg ccgcggccaa
     1561 gctgaccgta gggaaaggac gtggggaggc gggtcaggag ttcagcgcgc tggcgcgagt
     1621 acccctcggt attgacattg gcaatgttgt gcgcggcggt atcaatctgg atctgggcgg
     1681 ccgtgagccc agaccgcgca acatccaatg cactgaataa cgtgcccaat ttcatctcct
     1741 tcgcggccca tgccgcctgg cgcgcacagc accccggctg tcacggcgca aatcctgttc
     1801 caattatcgg cagccacata aaaggcttta gcgtgaaacg ggttgcgcag ggtggaccag
     1861 ggcggaccgc agcgtgaaat ggaaaaaacg ggaaaagcct gccgcgactc cggcaattcc
     1921 tgcagccgca aaatctcggc tattcgcgga cgtatcgggc caggaaggcg agcgcggctt
     1981 tgggcaggcc ctcgtgacct ccctcaaaga tcgtgacccg gcaggacccg gcgtaccggc
     2041 gcaggtagat ggttcgttcg aaggcgggat cctgctcttc ccggctggga agggattcct
     2101 cgcgggccag cgcgtccatc tccgccgcat tcacgagtgg cgcgccatgc gcgttcgcaa
     2161 ggacgttgaa ggcatcgagg gtgtggtgaa ccggcaccga gccggtgtgg ccatcatgaa
     2221 tgcccgtggc caggtcgaga ggcaggcctc tggcccggtg aagctggtgc acgggagacc
     2281 ggtaacgaag ctgctcgtcc actgcttgcg agctgccggg ggcgccgcca acgcacagct
     2341 cgatgtcgcg ggcgtacttg ttccccattt cgagcgtttc ccggtgccag gccgccaggt
     2401 ccgtgatcgc gcaccacgca gacgcggcag cccagcgttc gggcgcatgc gccgccatca
     2461 cgagggtcat gtgcccgccg cccgatgctc ccgccagata gacccgggcc gggtcgacgg
     2521 cgtgccgttg aaggacgtaa ccgaccgcat cgaggatgtc ctgccgggcc agaggcgacg
     2581 cgcaggcatc gggggtattg tttggcccgc gaaaatgagg ctggagaaaa tgccagttgc
     2641 gctgcgccgc ttcgtcccgc cacggctggg tatcaagatc gtagccgctg ctccaagtgt
     2701 ggagaaaaac cagcagaggc cggggtccgg cgccttgagc ctcgataaac aggctgggtt
     2761 gctcagttgc atcccctgtg ctggtcacgt aaatggtctt cacctcagaa acgctttcgc
     2821 tggcatgtgc cgcgcctgcc gcgatcaaaa acacgcccag gcacgtgaaa agcatccggt
     2881 tcttcgcctg cgggctcatt gttcgccgcg ggctgcattc acggcgtcgg caaatgcgcg
     2941 tgcgtttgcg gtgatcccgg caaaatcctg agccaagacc tgtttattgc tgacgagatt
     3001 gcccccaacc gccagcgccg ccgcgcctgc actcagaaac gcgcccacat tctccagttc
     3061 gacccccccc gtggggacca gaggcacctg aggcaacggc cctttgagcg ctttgatgta
     3121 gccggggccg cccacctgcg ctggaaatac tttgacaaaa tccgccccgt tttcccaggc
     3181 cgtcaggatt tccgtcgggg tatacgcgcc gcacaggatc ggtctaccgt aacgccgcgc
     3241 catctgaatc gtcgggaggg acagggtggg cgtaaccaca tagcgcgcgc ccgccaggat
     3301 ggccatgcgg caggtctcgg gatccaggac cgtgcccacg cccaggagca cgtcggcgtc
     3361 ccgcagcttt tccgacgccg ttttcaggca atcgagcgcg ccgggggtcg tcatagtcac
     3421 ttcgatgcaa tgcacaccgc cggctgcgac cgcctccacg accttcacga ggtcatcccc
     3481 gccacggtcg gcccgcacca ccgcgatgat gcattttttg aggagatagt gaatagcatc
     3541 gaacttattc atcgacagtc ccttcttttt gcgaatcacc cgcccgattc gaccacagtc
     3601 tagcggtgcg tgtcaaaaag gggtcctgaa agcagccccg cacgttccgg cgcgttcata
     3661 ccgcccggct tgaatcgagt gggttttccg gcataagacc ctgaagcgct tgtgtttccc
     3721 tttgagaaga ccagtctgga actcgcgcga atcccggtgc ttgtggtatc gtaacgggtg
     3781 ggcaacctga aggagatgta ttacctatgg caaccattcg aattgtcggt gtgcagatgg
     3841 aggtttctcc aaagctggat gtgaacctgc cgaaaatgat cgaccacatc atgaatgccg
     3901 atggcgattt tgtgcttttt cccgagatga gtctgacggg ctaccatgga aatttcaacg
     3961 acaaggcggt acgcgcggcc tgggaacaga tcgcgaaggc atgccggcgg aagtacatca
     4021 cggctctcat cggcacgggg cgcaagcgcg acgacgacgt gcatatccag acccgcatct
     4081 acaccgatgc aggggaactc ctgggcacgc acgaaaagct ggtgccgacc cgggccgatc
     4141 gcgaattctg caagccgggg gaaaaattgc gcattttcca ccaccgcggc ctgagcttcg
     4201 gatgcaccat ctgcaacgac ctctgggtga cccccggctc tggaccgtat cccgacccgc
     4261 ggctgaccta tcagttggga cagaaaggcg cccaggtcat ctttcattcc gcgaattccg
     4321 gcgccgatcc caggttccgg acttttcacg agtccaacct gcagctgcgc gccattgaga
     4381 gccaggtata tatcgccacc gcaaacgcgg ccagccccga cggccccgtg aattccccct
     4441 ccggaatcat gtcgcccgaa ggtcaatggc tggtgcaatg ccctctggaa ggtgagcaga
     4501 cctattcgca cgacatcgac gtggagatcg atgtgggcgc cgaagaagaa ggcgaggcgg
     4561 tgaaataacc gcgcgcgaac gggtaaacgt ggtagaatcc actggatgcg cggcatgaca
     4621 tcacagcgcg cggttgaaat atagaaaagc agctacgcct gtgaagcaag agaagtgcat
     4681 gaggcggtat tcatgacgac gggcacccat tcccggcgga ggttcctggc ccggtccacc
     4741 gccctcggcg ccgcaaccgc cctggcggcg cgcggcgccc gtgcgagcga aaaatcgcgc
     4801 cctgaaccga aaacaccggc ggaaccactc cgctgcgccg tgatcggtgt cggtaaacgc
     4861 ggtggggcgc tccttcagac tctgctcgaa ctggactccg tccaggtcgt ggcgcttgcc
     4921 gacacctatg acgtgtggcg gaaccgcgcc ctggcctggt gcaaacaaaa aaatggggag
     4981 acttccgact acgtcgagta cgaacacctg ctgaaaaaag aaaagctgga tgcagtgttc
     5041 atcgcaaccc cgccgcatac cctggctccc gccgcgctgg aagctctcgg ccgtcattgc
     5101 gccgtgtata tcgaacgccc catggcgctc acctgggagg ccgccaaggc cgtccgcgcc
     5161 aaagcccgcg agacgggcgc cgtggttcag gtcggcagcc attaccgcag cacggacctg
     5221 tatctccggg cccgggagat cctggcctca ggtgagctgg gaaagctcgc catggttcag
     5281 gtgaatcact acgacatggg ccaggcgctg gatcccgaga gcctgcccat agaggccacc
     5341 gaggagagcc tgcactggcc cctcttcctc ggggatacgc gggaataccc gttcgatccg
     5401 ctccgctact tccactgggt gcatttcgaa gaatacagcg ggggactcaa tggaggcctg
     5461 ttggccgatc acctcgacac ctgccatttt cttgcggacg ccggcatgcc tgcgcgcgtg
     5521 atgtccgctg gctcgcttgc caagttcaac gatggccgtt cctgccccga caccgtcaac
     5581 accatcgtgg aatatcccaa cggcctccag tttacgtata ccgccaccct gaccaacgaa
     5641 cgattcggcg tggaagagcg ctacatcggc actggaggcg ccctttacat ccgcaacatg
     5701 caggaaatga cgatctacct cgaagatatc gaagaaaacc tgaagtccaa cgggcccaat
     5761 acccgcgcgc acatcgaaga ctttctcgat gcggtccggt cgggcggtac ccctcgtgca
     5821 accgtcgacg acggattccg cgccgccgcg gcctgccata tggccgtcct atccgatcag
     5881 accggtaaaa ccatcgcctg ggatgccggg gaagaggccg tcgccgtcta atcttcgcac
     5941 cttcgaattc ctggcgcccg gatttggacc gctcggtctc cgcgggcagc gatcagagtt
     6001 ccggcgcgga accgccttcc acctcgccag tagcgggatc gtagcgccag gcgcggcccg
     6061 ggtagggatg gggcgggaca aatacgagat cgcccccctc cacggccgcc tcgagtgaag
     6121 gcggccaggc gccggtgcgc gcctggtaac ggttcacgcc gttgcggatg cggtttatgc
     6181 gccgctccag cttctcttcc tgcagcgtgg tattgaggac ttcgcggccg gccagaaaaa
     6241 aacgcccgcc aagcggatcg tcgggcaaac gctcgatgat gccgcctgcg accaggtcgt
     6301 tcagggaatc cgggaggcgg tggtggcgct cttcgtagag ctgaacggct ttgccgagct
     6361 gccggcatac atcggcgatg gccagttcat gcagctttcg ttgtgccagt tcacggagca
     6421 ttttttcgct gctgttccgg gccatatctt cccacatggc gcgttcaaat tcgtcgaggt
     6481 cgtgttgccg ggcgatgttg gcggcgaggt cgatcacgta ttgtggggcc gtcccggtgg
     6541 caacggccag ttgcagaaac tgggcggcct gcgctggcga gccgggattc tccctccggt
     6601 tgagcaggta gaccatggcc atctcgtagg gcagtaccca tgcgccgggg ttgtggatca
     6661 tgccgcgttt catgaggtcg atgctggcgt cgctgtcggc tttgagggcc gccaggaata
     6721 ccccgccata gcggtatacg tctacaaaat acgggtcaag gcgggtgatg gtgtcgatca
     6781 tgtggttgag ccagacatac ttgtgctcgc ccttgaactc ctcggcggtg tactggatgc
     6841 attggagcca gagcaggtcg gcaaccacgc tgctcaggcc tccggtgaaa tggttgagca
     6901 gcttttcgtt gggcagatac agcagttcct cgtcaaaccc ggtcttgcgg gaggcatcca
     6961 tctgcctctg cgcggcgacg cagcacgcaa agccgatggc cacggccagc acccctgtga
     7021 gcagccggcg cgcgatcata cgtccttatc ctgaaaggcc agcgacgcga tgatgagcag
     7081 gacagcggtg tagacggcgc cataggccat agcccagagg atgtagcccg gcgccaccgg
     7141 cacgccgttt gccgcctcgg cgcgaatgtc aaagttggag aaattgggaa tgatgtaata
     7201 cacggcctgg agaacgcgct ttgtggcggt gccgtcaaaa tgtggcggca gttcggtgaa
     7261 gatcgtcgtt gcatgaccca caacataggt ggaaaagacg atgatcgccc cgagaatcgg
     7321 cgaggtcagg gcggagagaa gcacggccag cgcggtgatc agcagcaatt gccagccgat
     7381 cagcagtccc gccagaaaaa aggtggcgtc gaggctgccg cccagaacaa cgacatagac
     7441 cgccgcgaca agcaccatca ccgctgtcac gagcgcgagc agcgccgcca gccccagata
     7501 tttcccgaga atgaactccc agcgatgcat cggacggcac aggatggtgt agatcgtgcg
     7561 tttgtcgatc tctttgtaca ccagattcgt ccccacgaaa atggcgatca gcactccaaa
     7621 taccgatacg gccgcgagcg acaggtcttt gacgatcttg atatcctgcc cgatgctgat
     7681 ccaccccagc gctttcgaac cgaagatggc ggctgcggca aacagcagca gcacatatag
     7741 cactttgtcg cgcactgctt cccggaaggt attgcgcgcc acggccagca ctctcatgac
     7801 acggtctcct gttcgcgcac aaaatactcc tcgagcgatt cccggatggg cgtgaattcg
     7861 gccagggtgc cgccggactg caggacgtcc gccgcgaggg cgttggcggc ctccatgtct
     7921 ttcacgaaaa agtggcggcc ctgttcggat tcgcggatcc cgtcggagcg ggccgcgagc
     7981 acggtcgcgg ccgcctcgtt caagtcggcg gcgatgatct ccacgcggtt gacccggcgg
     8041 ttaagaagtt cgtcaagccg tccctgcgtg gtcaggcggc cctggacgag gatccccacg
     8101 cggctgcaga tctgctcgac atcgccaagc acatgcgagc tgaaaaagat ggtcttgccc
     8161 cgttcgtgaa acagcaggat caattcgcgg atcatgcggc ggcccagtgg atcgaggccc
     8221 gacatcggct cgtcgagaat gagtacttcg gggtcgccca cgagcgcaac ggcgaaaccc
     8281 agccgctgcc gcatcccttt tgagaagccg cggacccgct gcccggcaat gccgcgcaga
     8341 tccagcagat cggccagctg ttcccattcg gcggcacgct cggcgcgggt caatccatga
     8401 agttccccgt aaaagtccaa tgtctcgcgg ggcgtgaggt attcgtaaaa atagggattt
     8461 tccgggaggt agccgatgtg cttgcgggtg gcgggccggc gcgtgtcctg ccccatgaca
     8521 aacgcctcgc cttccgtggc gcggagcagg ctgcacagga tcttgatcgc ggtggtttta
     8581 cccgcaccgt tccggcccag aaacccgaac acctcattgg gcgcgacggc caggctgaga
     8641 ttatccagcg acttcaccgc gccgcgcccg atatgtcccc ggtagatttt cgtgaggttc
     8701 ctggtggcta ttgcgtccat acacaacctc tcggcaccgg tactgcccct gctctgttca
     8761 tttcagcgcg caacccgggc agagcgcgcc ggcatcggtg ccgcgtccgc cgctcccggg
     8821 ccgcacggct acagcttcaa attgtcctgt atgctccgga cggcgcgttt gacgtcgccg
     8881 gcatgtccga atgcgctgag gcggaaatag ccttcgccgc cagcgccgaa accgctaccg
     8941 ggtgtggtga cg
//
LOCUS       DAOPBQ010000069         8917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7364_length_8917_cov_4.003949, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000069 DAOPBQ010000000
VERSION     DAOPBQ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7364_length_8917_cov_4.003949"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PKW60_03630"
     CDS             complement(<1..858)
                     /locus_tag="PKW60_03630"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HPJ98552.1"
                     /translation="MSKKTCQHEVLLGEIEVNLAGMQYCRAHPALGEKVHFEREPDNT
                     HDRKAIRVENEDFAKVGYLPRQVASWLTPLIDQGTVYVDGTVAARDDTPFPSATLALS
                     LYVAPSKAEFLQTPRDPASLPEALHRVVLDGYNAMHDWRRPTVIRDAAKRLGELSRRD
                     LLPETRLLLALMPKFAQTLETHADIGALSVARSALAELEIGEGLHYRNATVFPLYLAN
                     GHERTYVLLEEALEAGTADVTETSHHGTVPEILIRNRGDRPILLPEGEILTGAKQNRV
                     VNITMLIAAQ"
     gene            complement(855..1256)
                     /locus_tag="PKW60_03635"
     CDS             complement(855..1256)
                     /locus_tag="PKW60_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HPJ98553.1"
                     /translation="MSSEQYVFVYGRLRSGFWNQFLLGARKSAGTGRTVENYALYVSA
                     MPYVRMDQAVSPIRGEVYAVSAQTLRDLDRLMAHPTWYRRYEVPVKLDDGPEITAWLY
                     ARPGTGGVLVENGDLWNREEAAAGSNLEQMP"
     gene            complement(1341..1697)
                     /locus_tag="PKW60_03640"
     CDS             complement(1341..1697)
                     /locus_tag="PKW60_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98554.1"
                     /translation="MRVSKATQLDITEHALDRIAHHTGLPPTRALAQSLFERSRQVKA
                     THMWALGYRPAYGKRLASGQKSWYFRFMLFGEELVAVVQEGESSGRYLWVTTYGATPQ
                     SDHFRWREPGLVGEVC"
     gene            2040..3629
                     /locus_tag="PKW60_03645"
     CDS             2040..3629
                     /locus_tag="PKW60_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPJ98555.1"
                     /translation="MVAQRKQHIVTYYGLDGACAAALALKRFPAANVLTTSARRVGET
                     LQQLSQSAVCDVHLCGVGTGCDWAEVRQACEAIRKRGGEVFWYCGRGYLDRDRDRYAE
                     VCTPVFIEAESNTGALAKHLQLTDDPVAGFLTELAPLDHNISRDKPFRKKTSEQEDWC
                     DFVAAAISQYFKYQDEEVYRTAIYSLANRSLTEEDRHTLDIYRQTGFKYVLHGRSLAL
                     KTLRERIKKCARINRPLIIMGESGVGKEHVAQLIREASARCHEPFIVVNCALYAGSAS
                     LANSDIFGHVKGAFTGAEKERAGKLSTAHKGILYLDEVGELPLEVQAKLLRVFEDGQV
                     TPEGADRPTGHVDVRIIAATNRELPEMVRAGTFRADLYHRLSTLRLFVPPLRDHLEDI
                     RSILEEHLISLAKEGYERTLTRGDYQALKGYDWPGNVRQLIKLVERAFYLDMSIAEAI
                     AEEEALGELRVAVDVRPSPDELLLPRTTSEVRPIEEVRNQYAQRAWELFDRNYTATAK
                     ALGIRPNTLRYKYLKEKKQQS"
     gene            complement(3679..5883)
                     /locus_tag="PKW60_03650"
     CDS             complement(3679..5883)
                     /locus_tag="PKW60_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPJ98556.1"
                     /translation="MSERLTRRGFIQHSVQGAAGTAALIGVLGSTRPAASVASDPGDA
                     KGVLAGAAAVDITPQVLPVISSGGFLEATADSITDPLFARGLVLDDGAKRLALVLVDN
                     LMMPRELLDDVKRRASAATGIREEHMLIAATHSHTAPSVMGALGSRCDEAYAAFLPDR
                     LVECIQQADANRVPARLGWAAVADYEDTNCRVWFRRPDRIDTDPFGEATIRAMMHPGY
                     QNPDYIGPCGPKDPALTVLAVRTVDGAPLALLANYSMHYFGSTPISADYYGRFCAEME
                     RRIAPGQDHPRFVALISNGTSGDQHWMNYAAPKTDLTIDAYAARVADKAMEAWQAAEY
                     IEDASIDMAEMTLTLDRRTPSPERLAWANDIRAAMGDRTLPKDKAEVYALEQLYLAAE
                     PRRELKLQALRLGGLAIAAIPCEVYAITGLKLKRQSPFPMTMNIELANGAEGYIPPPE
                     LYCFGGYNTWPARTAGLEPAAEPRITEALLKLLEGLADRSRRPLPICAGPYVDAVRAS
                     GPEAYWPMDTLTGTTCADLSGNGHVARCEPGVAFFLEGPSLKETGHPDGICRALQCAG
                     GRVAASIPVPGDAYSVELWFWNGLPSDARAMTGYIVSRGRDREPGAPGEHLGIGGTDA
                     GGQALGRLIAYNGDTKGTLLAGATELEPKRWYHVAYVRQGLDIAVYLDGNATPEIAGT
                     LEPGCSPDIAGLWMGGRSDALFGLEGRLCHAAVFGRALTPAEIASHYRAAMA"
     gene            complement(6013..8778)
                     /locus_tag="PKW60_03655"
     CDS             complement(6013..8778)
                     /locus_tag="PKW60_03655"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HPJ98557.1"
                     /translation="MRQRFLFLSALVVLAIGVGCPFQPPGKSDYRFTSADLRQSNNAW
                     WTWGGHPAAGRDEDSQVGEENVPREVTEPDVFRRDGNLLYVLNQYRGLTLVDLDTEKV
                     LAQLPTFGYPRDLYVRDGRAYVLVGQATHYETNGNTVSYTVQGRLYIADVSTPASPSI
                     LSSIALDGDFVDSRLVGDVLYAVCAEFDWYWDGVVSTKGQTSATWVTSVNVADPDNVY
                     PADQLSFGGAGDVIQATSTAIFVASTPAWSGEQTTITYVDISDPAGAMAVRGAVNVNG
                     YVADRYKLDAWEGALRVVSSAWNDERRVYVTTISLADPDHLTVLAELPLERATGETLF
                     ATRFDGPRAYIVTYLIQDPLFVVDLSDPSAPTLLGELEVPGWSTHIEPRGDRLLALGV
                     DDTGGRRVCVSLFDVADPGGPVLVDRETFGDGWSWSSAYGDVKAFAVFDDLLIVPFSG
                     WSGAGGYERLQFVSWSPDELDARGYVDLTGTSIRSFDYEGNYYGLTTEQLVTIDATVL
                     DAPSVTNRLVLAEYLADFVEITPHVGAEIVARYDSGTTRVRTVSLAKAAGGEVEVKLG
                     DLVDSLVYGDSVVLVGAKWDDSPQYVVAVVDCSDPEQPRLVFNERIPVEPYYGWYWPL
                     YPYRPLLEVDSAASADKSIVARPWYPSSQTGSAFLLGDVLALRCRADKYGSVLGPDTA
                     EQGLALIDLAAGKFTGTVGLAFDHIVSLDVADAGLYLSTCEPLKTVAGMERPKCAYFI
                     TAIDVVGTPSAGPSANVPGIFVQYDPGSGLLTLRDDQWLRAWEYRSHLRTVRWNGGEK
                     VESLDSAQLPDGASQVLGRGAKVYVESYADGLKLYAVAVLPSGALKPGSGVLVTRDWG
                     SLLDARDSSAYVVLGGSAVARYDFSSGAGTLGEIMPVMGYPLRIRFGASAAYVSTGYS
                     GVLELPL"
BASE COUNT         1678 a         2815 c         2850 g         1574 t
ORIGIN      
        1 ctgcgccgcg atgagcatgg tgatgttgac gacccggttc tgcttcgcgc cggtaagaat
       61 ttcgccttca ggcaagagaa tggggcggtc gccgcggttc cggataagga tctcgggtac
      121 ggtgccgtga tgcgacgtct cggtgacgtc ggccgtcccg gcctcaagcg cctcttccag
      181 caggacgtag gtgcgctcgt ggccgtttgc gagatacaac ggaaagaccg tcgcgttgcg
      241 gtaatgcagg ccttcgccga tttccaactc ggccagagcg ctgcgtgcta cgctgagcgc
      301 gccgatatcg gcatgagtct ccagcgtctg agcgaatttc ggcatcagag ccagcagcag
      361 gcgcgtctca ggcagcaggt cacggcgtga aagctcgcca aggcgtttcg cggcgtcgcg
      421 gatgaccgtg gggcggcgcc agtcgtgcat ggcattgtag ccgtcgagta cgacgcggtg
      481 cagcgcctcg gggaggcttg ccgggtctcg aggcgtctgg aggaattccg ctttggacgg
      541 cgcgacatac agcgacagcg ccagtgtggc cgaaggaaaa ggagtgtcat cacgcgcggc
      601 caccgtaccg tcgacataga cggtgccctg gtcgatgagg ggcgtgagcc aggatgcaac
      661 ctgccgggga aggtagccca ctttggcaaa gtcttcgttt tcaacgcgaa tggccttgcg
      721 atcgtgggta ttgtcgggct cgcgctcaaa atgcaccttt tcgcccagcg ccgggtgtgc
      781 tctgcaatac tgcattcccg ccagattgac ctcgatttcg ccgagcagta cttcgtgttg
      841 acaggttttt ttgctcatgg catctgctcc aggttggatc ctgcggccgc ttcttcacgg
      901 ttccacagat ccccgttttc caccaaaaca ccgccagtcc cgggacgtgc atacagccac
      961 gccgtaatct cgggaccatc gtccaatttc accggcacct cgtaacgccg gtaccaggtg
     1021 ggatgcgcca tcaggcgatc caggtcgcgg agtgtctgtg cgctgaccgc gtacacttcg
     1081 cctcgtatgg ggctgacagc ctgatccatc cgcacatagg gcatggcgct cacatacagg
     1141 gcgtaatttt caaccgtgcg ccccgtcccg gccgatttcc gggcccccag caagaactgg
     1201 ttccagaaac cgctccgcag cctgccgtaa acaaagacat attgctcgct actcatgccc
     1261 gcaccctccg gcttctccat acttccgaga acgcccatga cgggacggcg cctcgtcccg
     1321 cggcgccgtc ccccgggcct tcagcagacc tctccaacga ggccgggttc cctccagcgg
     1381 aaatggtctg actggggagt cgcaccgtag gtagtaaccc acagataccg gccggaagac
     1441 tccccttcct gaaccaccgc caccaattct tcgccgaaca gcatgaaacg gaaataccag
     1501 gacttctgcc cgctggccag gcgcttccca taggccggcc ggtagcccag cgcccacatg
     1561 tgggtggcct tgacctgccg gctccgctcg aacagggact gtgcgagcgc ccgcgtgggc
     1621 ggaagtcccg tgtgatgcgc gatgcgatcc agcgcgtgct ctgtgatatc cagctgtgtc
     1681 gccttcgaga ctctcatgaa tcctttccct gtgatagcca tcgctgcgcc cttccttgtg
     1741 tttactgccc catcaacttc gatgccctct ggctgtgttt gcgccaaacg cctctaagac
     1801 agcaggtatc gtgccaatac agcagcccgc gcgtagcacc atatgtgggg tgatttcgag
     1861 gaaagagcac aaaagatggc gtgttgtgtc ctcaatatta acgtgctatg ctcaaaaaac
     1921 gagtagttgc aaacggccgc caggcatgcg agcccaatca ggcactccag ggcgctgctg
     1981 cgcatgcggg cgcatttccc gggggtaatg ccgggttcat cccggcggaa aggagccaga
     2041 tggtggcaca gagaaagcag catatcgtga cctattacgg gctggatggc gcctgtgccg
     2101 cggccctggc gctgaaacgg tttccggcgg ccaacgtgct gacgaccagt gccaggcggg
     2161 taggcgagac attgcagcag ctcagtcagt ccgcggtatg tgacgtgcat ctatgcggag
     2221 tgggaaccgg ctgcgactgg gccgaagtga gacaggcctg cgaggcaatc cgtaaacgcg
     2281 ggggagaggt gttctggtac tgcggccggg gctatctcga cagagaccgg gatcgttacg
     2341 cagaggtctg tacgccggtc tttatcgagg cggaaagcaa tacgggcgcc ctggcaaaac
     2401 acctgcagct caccgacgac ccggtggccg gatttttgac cgagctggcg ccattggacc
     2461 acaacatctc gcgcgacaag ccttttcgaa agaaaaccag tgaacaggaa gactggtgcg
     2521 attttgtggc ggccgcgatc tcccagtatt tcaagtacca ggatgaggag gtctaccgga
     2581 cggccattta cagtctggcg aatcggagcc tgaccgagga ggaccggcac acgctcgaca
     2641 tctaccgtca gacagggttc aagtatgtat tacacggccg atcgctggct ctgaagacac
     2701 ttcgtgagcg gatcaaaaag tgcgcccgga tcaaccgccc gctgatcatc atgggcgaat
     2761 cgggagtagg aaaggagcat gtggcgcaat tgattcgcga ggccagcgcg cgctgtcacg
     2821 agccgtttat tgttgtgaat tgcgccttgt acgcgggcag cgccagtctc gctaactccg
     2881 atatcttcgg ccatgtgaaa ggcgcgttca cgggcgcgga gaaagagcgt gcgggcaagc
     2941 tcagcacagc gcataagggc atcctctacc tggacgaggt cggcgaactc ccgctcgagg
     3001 tccaggcgaa attgctgcgg gtgtttgaag acggccaggt tacacccgaa ggggcggatc
     3061 ggcccacggg ccacgtcgac gtgcgcatca tcgccgcgac taaccgggaa ctgcccgaaa
     3121 tggtgcgcgc gggtactttc cgcgcggatc tgtatcaccg cctgagcacc ctgcgccttt
     3181 ttgtcccccc gctgcgcgat cacctcgaag acatccgttc catcctggaa gagcacttga
     3241 tatcgctcgc aaaagagggg tacgagcgca ccctgacgcg cggcgattac caggcgctga
     3301 agggctacga ctggccggga aacgttcgcc agttgatcaa actggtggag cgtgcgttct
     3361 atcttgatat gagcatcgct gaagcgattg cggaagaaga ggcgctgggt gaactccgtg
     3421 ttgccgtgga tgtccggccg tctccggacg aattactgct tccccgcacc acgagcgagg
     3481 ttcgcccaat cgaagaagtg cgcaatcagt atgcacagcg cgcctgggaa ctctttgacc
     3541 gaaattacac ggccaccgcc aaagctctgg gaatccggcc caatacgctg cggtacaagt
     3601 atctgaaaga gaaaaaacag cagtcctagc cggcctgcct cgtgccgggc cgtgcgtgtt
     3661 gtgccgctct gcctgttgtt acgccatcgc ggcgcggtag tggctcgcga tttccgcggg
     3721 cgtgagtgcg cggccaaaca ccgctgcgtg gcagagcctg ccttcgagtc cgaagagggc
     3781 gtcgctgcgg ccgcccatcc acagaccggc gatatccgga gaacaaccgg gttcgagcgt
     3841 gcctgcgatt tccggagtgg cgttgccatc cagatacacg gcgatgtcga ggccctggcg
     3901 gacgtacgcg acatggtacc agcgcttggg ttcgagctcg gtcgcgcctg cgagcagggt
     3961 gcctttggtg tcgccgttgt aggcgatgag ccgtcccaac gcctggccgc cggcatccgt
     4021 tccgccaata ccgagatgtt cgcccggcgc gccgggttcg cggtcgcggc cacgggagac
     4081 gatgtagccc gtcatcgcgc gggcatcgct gggcaggccg ttccagaacc agagctcaac
     4141 cgaatacgcg tcgcctggaa cgggtatgga ggcggccacc cggcctcctg cgcattggag
     4201 ggcgcggcag atgccgtcgg ggtggccggt ttccttgagg gaaggccctt caaggaagaa
     4261 cgccacgccc ggttcgcaac gagccacgtg gccgttgccg gacaggtcgg cgcaggttgt
     4321 gcccgtgagg gtgtccatgg gccagtaggc ctccggcccg gaggcgcgca cggcatcgac
     4381 ataggggccc gcacagattg gcaggggccg tcgcgagcga tcggccagac cttcgagcag
     4441 cttcaacagg gcttccgtga tcctgggttc cgcggcaggc tcgaggccgg cagtgcgcgc
     4501 gggccaggtg ttgtagccgc cgaaacaata cagctcgggc ggcgggatgt agccttcggc
     4561 gccgttggcc agctcgatgt tcatggtcat ggggaacggg ctctgccgtt taagtttgag
     4621 gcccgtgatg gcatagacct cgcaggggat ggcggcgatg gccagcccgc caaggcgcaa
     4681 tgcctggagt tttagttcgc gccggggttc cgccgcgagg tagagctgtt ccagggcata
     4741 cacctccgcc ttatccttgg gcagggtacg gtcgcccatg gccgcccgga tgtcgttggc
     4801 ccaggcaagc cgttcgggag acggagtccg gcgatcgagc gtgagtgtca tttccgccat
     4861 atcgatcgag gcatcctcga tatactcggc agcctgccag gcctccatgg ctttgtcggc
     4921 cacacgggcg gcataggcgt cgatggtgag atccgttttc ggcgccgcat aattcatcca
     4981 gtgctggtcg ccgctcgtgc cattggagat cagggcgaca aaacgcggat ggtcctggcc
     5041 gggcgcgatg cgccgctcca tttccgcgca gaaccggcca taatagtctg cggaaatggg
     5101 cgtggaaccg aaataatgca tcgaataatt ggcgaggagc gccagcggcg cgccatcgac
     5161 cgttcgcacc gccagcacgg tcagcgcggg gtctttcggg ccgcacggcc caatgtaatc
     5221 gggattttgg tagccggggt gcatcatcgc gcgaatggtg gcctcgccga acggatccgt
     5281 gtcgatccgg tcggggcggc ggaaccatac gcggcagttg gtgtcttcgt aatcggccac
     5341 ggcggcccag cccaggcggg cgggtacacg gttggcatcg gcctgctgga tgcactcgac
     5401 gagccggtcc ggcagaaagg ccgcatacgc ctcgtcgcag cgagatccca gtgcgcccat
     5461 gacggacggc gccgtgtgtg aatgggtcgc ggcgatgagc atgtgttcct cgcggatgcc
     5521 cgtcgcggcg ctggcgcggc gttttacgtc atccagcagt tcgcgcggca tcatgaggtt
     5581 gtccaccagc accagggcga gccgtttggc gccgtcatcc agtacgaggc cgcgcgcgaa
     5641 cagcgggtcg gtaatcgaat cggccgtagc ctccagaaaa ccgccgctgc tgatcacggg
     5701 caagacctgg ggcgttatgt ccacggccgc ggcgcccgcc aggactccct tcgcgtcgcc
     5761 tggatccgaa gcgacgctcg ccgccgggcg cgtggaaccc agaacgccaa tcagcgcagc
     5821 cgtgcccgcc gcgccctgca ccgaatgttg aatgaatccc cggcgtgtca gtcgttcgct
     5881 cacgatcctg tcctccgctt gcttgtccgt aaaacagact acccgttcac gcgcggggaa
     5941 cacaagatgg tccgcggagt attcgcggcc ggaaaaagcg ccggacccgc caggaactcg
     6001 cgcaggcttc tgctacaggg gcaattcgag cacgccggag tagccggtcg aaacataggc
     6061 cgccgaggcg ccgaaacgaa tgcgtaacgg gtaacccatc acgggcatga tctcgcccag
     6121 cgtgccagcg ccggaactga aatcgtaccg ggccaccgcg ctccctccga gaacgacata
     6181 ggcactcgag tcccgggcat cgagcaaact gccccagtct ctggtgacca gcacgccgct
     6241 gcccggtttc agggcgccgc tgggcaatac cgcgaccgca tagagcttca ggccgtcggc
     6301 gtaggattcc acatacacct tggcgccgcg cccgagcacc tggctggcgc cgtcgggaag
     6361 ctgagccgag tcaagggatt cgaccttctc cccgccgttc cagcgcaccg tccgcagatg
     6421 ggagcggtat tcccaggcgc ggagccactg atcatcacgc aacgtcagca ggccgctgcc
     6481 gggatcgtac tgaacgaaga tgcccggaac attagcgctc ggaccggccg acggtgtccc
     6541 gaccacgtca atcgccgtga tgaaatacgc gcacttcggc ctctccatgc ctgcaacggt
     6601 cttgagcggc tcgcaggtgc tcagatacag accggcgtcc gccacgtcga gcgagacgat
     6661 atggtcaaac gcaaggccca ccgtgccggt gaactttccg gccgccaggt caatcagcgc
     6721 gagtccctgt tccgcggtat ccggacccag aacgctgccg tacttgtccg cacggcagcg
     6781 caatgccagc acgtcgccca gcaggaacgc ggaaccggtc tggctcgagg gataccaggg
     6841 ccgcgcgacg atggatttgt ccgcggaggc ggcgctgtcc acttcaagaa gcggccggta
     6901 cggatagagt ggccaatacc acccgtagta cggctcaacg ggaatgcgct cgttgaacac
     6961 gagccgcggc tgttctgggt cggaacaatc gacgaccgcc accacatact gcggcgaatc
     7021 atcccacttc gccccgacaa gcacgaccga gtctccgtag accaggctgt cgaccaggtc
     7081 gccaagtttg acctcgacct cacctcctgc cgctttcgcc agcgacaccg tccgaacgcg
     7141 tgtcgtgccg gaatcataac gcgcaacgat ttcggcgccg acgtgcggcg taatttccac
     7201 gaaatcggca aggtattccg ccaggacaag gcggttcgtc acgctgggcg cgtcaagcac
     7261 ggtggcgtcg attgtgacca gttgctccgt cgtgagaccg tagtagttgc cctcatagtc
     7321 aaaagaacgg atggatgtgc ccgtgaggtc cacgtaaccg cgcgcatcga gttcgtccgg
     7381 cgaccatgac acgaattgaa gccgctcgta gccccccgcg ccgctccagc cgctgaacgg
     7441 cacgatgagc aggtcgtcaa acaccgcgaa ggccttgacg tcgccgtagg cgctggacca
     7501 gctccacccg tccccgaacg tctcgcggtc aacgagaacc gggccgccgg ggtccgccac
     7561 gtcgaacagg ctcacgcaca cacgacggcc gccggtgtcg tccacgccca gggcaagcag
     7621 gcggtcgccg cgcggctcga tgtgcgtgga ccagccgggc acttccagtt cgccgaggag
     7681 ggtgggcgcc gacgggtcgg acaggtccac cacgaacagg gggtcctgga tcagatacgt
     7741 cacgatgtag gcccgaggcc cgtcaaaccg ggttgcgaag agtgtttcgc ccgtggcgcg
     7801 ctcgaggggc aactcggcaa gcaccgtaag atggtccggg tccgcgaggc tgatggtggt
     7861 cacatagacg cgccgctcat cgttccaggc gctggatacg acgcgcaagg cgccttccca
     7921 ggcgtccagc ttgtaccggt ccgcgacgta gccattgacg ttcaccgcgc cgcgcaccgc
     7981 catggcgccg gccggatcgc tgatgtcaac gtaggtgatg gtggtctgct ccccggacca
     8041 cgcgggcgta gacgccacga aaatggcggt ggaggtggcc tggatcacgt cgccggcgcc
     8101 tccgaacgag agctggtccg cggggtagac gttgtccgga tcagcgacat tgacgctggt
     8161 tacccacgtc gcgctggtct ggcccttcgt agagacaacg ccgtcccaat accagtcgaa
     8221 ttcggcgcac accgcataga gcacatcgcc gacgaggcgg ctgtcgacga aatcgccgtc
     8281 cagggcgatg gaagaaagga tcgatgggga cgccggggtc gaaacgtcgg cgatgtagag
     8341 ccgtccctgt accgtatagc tcaccgtgtt gccgtttgtt tcgtagtgtg tcgcctgtcc
     8401 gaccaggacg taggcgcggc cgtcgcgcac gtagagatcc cgcggatacc caaaggtcgg
     8461 aagctgcgca agcacctttt ctgtatccag atcgaccagg gtcagcccgc ggtactggtt
     8521 caggacgtac agaaggttcc cgtcgcgccg gaagacatcc ggctcggtca cctcccgcgg
     8581 gacgttctcc tcgcccacct ggctgtcttc atcgcggccc gcagcgggat gccctcccca
     8641 cgtccaccag gcgttgttgc tttgccgcag gtcggcactg gtgaaccggt aatcggactt
     8701 ccccggcggc tggaacgggc atccgacacc gattgccagc accaccagag cgctcaggaa
     8761 caggaacctc tgacgcatga ccgttctccc tggaaggtga gcgcgccgga agcggttcgc
     8821 ggatccagac cgcatccacg aaggcccggg ccgcccatgc ctgccgcgaa ggatgcacat
     8881 ctccctctat tctactcccg gaagccgccg ttgtgac
//
LOCUS       DAOPBQ010000070         8816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7459_length_8816_cov_4.337062, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000070 DAOPBQ010000000
VERSION     DAOPBQ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7459_length_8816_cov_4.337062"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="PKW60_03660"
     CDS             complement(<1..1434)
                     /locus_tag="PKW60_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPJ98558.1"
                     /translation="MITREDALKYHGQGRRGKIEVIPTKPCHTQRDLAMAYTPGVAEP
                     CREIEKDPSLAYEYTARANLVAVVTNGSAVLGLGSIGALAGKPVMEGKGVLFKRFADI
                     DVFDIELDTSDPEEIIRAVKLMEPTFGGINLEDIRAPECFEIEERLKAEMDIPVFHDD
                     QHGTAIISGAGLINALQIAGKDIEEVKVVFNGAGAAGVACAKFYIALGVKPENVVLCD
                     RVGVIYKGRTEDMSPHKEAFAVETDARTLADVMRGADVFAGLSVGNCVTREMVRSMAD
                     RPILFAMANPDPEIAYDEAMSARDDLIMATGRSDFPNQINNVLGFPFIFRGALDVRAR
                     AINDEMKIAAAYALANLTKEPVPYQVEKIYRGERLRFGPKYIIPKPFDPRVLIWEASA
                     VAQAAMETGAARIKLDIDEYREMLEARLGLSQKVMRVVINKARREPKRIVFPEGDQIP
                     VIKAVETILDEGMATPILLGPRERIEAM"
     gene            complement(1624..2025)
                     /locus_tag="PKW60_03665"
     CDS             complement(1624..2025)
                     /locus_tag="PKW60_03665"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPJ98559.1"
                     /translation="MKVKYWFWQTEFPVAPEQFHELDESHSPWYETPEEIEAALDYGR
                     EKARLLRWVRRHMRTRLTPRERRAVHLIFFRGMTYVEAARETGVHPSVVHRTVQRGLA
                     KLRRQLMTGRTRGRLRITQFRSPGKDAEPHR"
     gene            complement(2155..3486)
                     /locus_tag="PKW60_03670"
     CDS             complement(2155..3486)
                     /locus_tag="PKW60_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPJ98560.1"
                     /translation="MLEFRRCFAVRVLLAAALSLAFLPAFLARAATDWPDFRGPWHDG
                     HVQAPGSTEPAALPLTWSETGNVAWKTAIPHEGLSTPVILNGQIWLTSATEDGRQSFA
                     LCVDAETGEFLFNETIFETENPEPLGNNINTYASPSPVIEPGRVYVHFGSYGTACLDT
                     ATAKPIWTRRDLPCRHYRGPGSSPLLYGDLIILTFDGIDQQYVAALNKNNGETVWKTD
                     RSTEWKDLQDDGQYVQGGDFHKGFCTPIVFEWEGRPQLISPAACAAFAYDPLTGAELW
                     KTDHNGHSASPRPLFGHGLVYVVTGHRQTELWAIRPGGQGDITGTHVAWRYTGKEVPK
                     QPSPLLIGDLIYMIGNEGHAACLEALTGAVVWSERIGGNFMASPIYADGRIYITSMQG
                     ETTVLKAGRDFEVLARNRLESGCLASPAVSGDALFLRTKTHLYRLETLPQP"
     gene            complement(3568..5337)
                     /locus_tag="PKW60_03675"
     CDS             complement(3568..5337)
                     /locus_tag="PKW60_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98561.1"
                     /translation="MVFLVLPVAALALGSWALHPFGIKRGAAGQLARLCTGLALCGGI
                     VLLAGGISLALARWALIAIAVGGLTCEVFRRKERDSVDASLTEPPARKSSAALFWLGA
                     AAAASGMVLAAVPACVPATSLETVSGALAAAKDCDFQHRMVPYEGAETPAYATLVQCL
                     YTWAYASGGEGGARVLSWLTAVFAILSIHVLACRLSGPCAGAVAAGAFAATPAFLNRV
                     AAVSGDLLGLGLILAALSALLIWRAERAGVWLVLAAIFLGSAWDGRWTALILCCAAPL
                     FVACLAKDNRGKQSLVFASALAASLAPWVVTRALAGAPFILPVASETQHAAETGLLAW
                     LKFPWNLILRPHWYGGWTTAPSGLLLIFGLPGLVVGGKRVRWLCGFATVCGLALYFVD
                     RSHVAFLPLTSIFVVAAAVGACRLTALRPLVFVALTAVLLQGLCINGWTAAAALHGAG
                     RAGFLAQRVPRYEAIEWANNNLPGGRRILTPEPYHYFFDSQAFRNLRALAPLNGADPA
                     TLRDWLAQRGIAYLVFPETYVRAHRDACPEGAVELLDQWRGRQSEVFALIQVLKVPAL
                     DGTGLEQVEIYEVRNTAAKEPAP"
     gene            5701..6984
                     /locus_tag="PKW60_03680"
     CDS             5701..6984
                     /locus_tag="PKW60_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPJ98562.1"
                     /translation="MSTIPRPAPVQGDIQWFVESRFGMFIHWGLYALPARHEWIRHGE
                     EIPDDAYAPYFKFFEPDLFDPGAWARMAREAGMKYVVITTKHHEGFCLFDSQYTDYKA
                     PNTPCRKDLIEEVVDAFRAEGLRIGFYYSLIDWHHPDFPIDAIHPLRNHPDAHDLNRS
                     RDMARYREYMFHQVRELLTNYGKIDIMWFDFSYPNERLNGLGGKGRDDWHSEALLKMV
                     RELQPHVIVDNRLDLPPDMADIHTPEQFQPREWVTVDGKPVYWEACQTFSGSWGYYRD
                     EDSWKSPEQLIQMLINTVSCGGNLLMNVGPTARGLFDSRARNALKVYRDWMALHSRSI
                     YGCTQSEFAAPEHGRLTQNGKRLYVHLFAWPFKHLHLDGLAGKVEYAQLLNDASELRI
                     HKNAGSTDDGRATLTLDLPVKKPEVVVPVVELFLK"
     gene            7014..7697
                     /locus_tag="PKW60_03685"
     CDS             7014..7697
                     /locus_tag="PKW60_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPJ98563.1"
                     /translation="MDENTVILDAIPFEVDEAGALAALQLTPDSPFAQEAVALLHEAA
                     SVARPKGMYRLSAVETIDSNTTALDGVQFTSRILRINLTGLSRAFPFVATCGVELEEW
                     SRSKTDVMHQFWADGLKERALQSAIGALETHLAARYQSGTRGMMNPGSLVDWPLSEQP
                     RLFSLFGDACGRTGVRLSESCLMIPVKSVSGIWFETETEFVNCQLCPREDCPKRRTPF
                     DARALEHYA"
     gene            7840..7916
                     /locus_tag="PKW60_03690"
     tRNA            7840..7916
                     /locus_tag="PKW60_03690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7874..7876,aa:Arg,seq:acg)
     gene            8087..>8816
                     /locus_tag="PKW60_03695"
     CDS             8087..>8816
                     /locus_tag="PKW60_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPJ98564.1"
                     /translation="MDWAELAEWIHRVKESDLPAPPVPLAPAIQIRDVSRYLAALRND
                     VAKGADSARARTGALQREIKRLMDIAREVTAPPRESPAVAAPAEPPAEAGLPVSPAGP
                     VIVAFDLETTGLDPNENDIIEVGGVKFTPQGDVLGRFEQFANPGYAIPAESTAVSGIT
                     TEMVASADPSFDVLKAFIEWAGPDAVYAAHNAAFDAGFIEATYEKAGRKCPPLKVADT
                     LEWARSLKLETPNCKLGTLLAHYHK"
BASE COUNT         1704 a         2770 c         2761 g         1581 t
ORIGIN      
        1 catcgcctcg atgcgctccc gcggaccgag caggatgggc gttgccatcc cctcgtcgag
       61 aatggtctca accgctttga tcacgggaat ctggtcgccc tctggaaaca cgatgcgctt
      121 gggttcgcgg cgggccttgt tgatgaccac ccgcatgacc ttctgcgaca ggcccagccg
      181 cgcctcgagc atttcgcggt attcgtcgat gtcgagcttg atgcgggcgg cgccggtctc
      241 catggctgct tgggccacgg cggaggcctc ccagatcagc acgcgcggat cgaaaggttt
      301 cggaatgatg tatttcggcc cgaaacgcag ccgctccccg cggtagatct tttcgacctg
      361 atagggcact ggttccttgg tgaggttggc cagcgcgtag gcggccgcga ttttcatctc
      421 atcattgata gcgcgggcgc gcacatcgag cgcgccccgg aaaataaacg gaaacccgag
      481 cacattgttg atctggtttg gaaaatcgct gcgtccggtc gccatgatca ggtcgtcgcg
      541 cgcactcatg gcctcatcgt aggcgatctc ggggtcaggg ttggccatcg caaacaggat
      601 cggccggtcg gccatggacc gcaccatttc gcgggtaacg cagttgccca ccgaaaggcc
      661 tgcgaacaca tcggcgccgc gcatgacgtc cgccagcgtg cgcgcatccg tctcgacggc
      721 aaacgcttcc ttgtgcgggc tcatgtcttc ggtgcggccc ttataaatga cgccgacgcg
      781 gtcgcagagc accacgtttt cgggcttcac cccgagggcg atatagaact tcgcgcacgc
      841 gacccccgcg gcgccggctc cgttaaacac caccttgacc tcttcgatgt cttttccggc
      901 gatctgcagg gcgttgatga gtccggcgcc ggaaatgatg gcggtaccgt gctgatcgtc
      961 atgaaaaacc gggatatcca tctcggcttt gagccgttct tcgatctcga agcactcggg
     1021 cgcgcggatg tcctcaagat taatgccgcc gaaggtgggt tccatcagct tgacggcacg
     1081 gatgatctcc tcggggtcgc tggtgtcgag ctcgatgtca aacacgtcga tatcggcgaa
     1141 acgcttgaac aggacgccct tgccttccat gacgggtttg cccgcgagtg cgccaatgct
     1201 gcccaggccg aggaccgccg aaccgttggt gaccaccgcc accagattgg cgcgggcagt
     1261 gtattcatag gccaggctgg ggtctttttc gatttcgcga cagggttcgg ccacgccggg
     1321 ggtataggcc atggccagat cgcgctgggt gtggcagggt ttggtcggga tgacctcaat
     1381 tttgccgcgc cgcccctggc catgatactt caatgcgtct tcacgggtga tcattgggtc
     1441 ctcctgtcgc cagccaatcg ctcgaaacgc acccggcgtg cgcggagctg tgcgggattc
     1501 acgtcgatag cgcgctcgtg gaattgccgg gcgaaaatgt ccggtaatcg ggccgaacgc
     1561 ctccggcggc agacagccaa aacaccgctc agcgccggat ctttcgtggc gcaagtatac
     1621 tccttacctg tgcggttccg catccttccc cgggctgcgg aactgcgtta tgcggaggcg
     1681 tccccgcgtg cggcccgtca tgagctgacg gcgcagtttg gcgaggccgc gctgaaccgt
     1741 gcggtgcacc accgacggat gcacgcccgt ctcgcgtgcg gcttcaacgt aggtcatgcc
     1801 gcgaaagaag atcagatgga cggcgcgccg ttcccgtgga gtgagccgcg tccgcatgtg
     1861 ccggcgcacc cagcgcagaa gccgcgcctt ctcgcgtccg tagtcgagcg ctgcttcaat
     1921 ttcttcgggc gtctcgtacc aggggctgtg tgattcgtcc agctcgtgaa actgctcggg
     1981 agcgacggga aactcggttt gccagaacca gtacttcact ttcatgcgca gatacctccc
     2041 cgggacggcg tcggcgccgg cccgcgtttg tccgggaggc atcgtgacag ggaaaggcgt
     2101 gaaaagacct ttgcgaaaag cgggcgtttt ctttgattat tcttgtcagg cgccttacgg
     2161 ctgcgggaga gtctcgaggc ggtagaggtg ggtcttggtc cgcaggaaaa gcgcatcgcc
     2221 cgacacggcc ggtgacgcga ggcagccgga ttcgaggcgg ttgcgggcca ggacctcgaa
     2281 atcgcgcccg gccttcagga ccgtggtttc gccctgcatg ctggtgatgt agatgcgccc
     2341 gtccgcgtag atgggcgatg ccatgaagtt gccgccgatg cgttcgctcc agaccacggc
     2401 tccggtgagc gcctcaagac aggcggcgtg gccttcgttg ccgatcatgt aaatgaggtc
     2461 cccgatgaga agcggtgacg gctgcttggg cacttccttg ccggtgtatc gccaggcgac
     2521 atgcgtgccg gtgatgtcgc cctggcctcc ggggcggatg gcccacagct cggtctgccg
     2581 gtggccggtg actacgtaca ccaggccgtg gccaaacagg ggccgcggcg atgcggaatg
     2641 cccgttgtga tccgttttcc agagctccgc cccggtgagc gggtcatacg cgaaggccgc
     2701 gcaggcggcg ggcgagatca gctgaggccg gccttcccat tcgaacacga tgggcgtgca
     2761 gaatcctttg tggaagtcgc ctccctgcac atactggccg tcatcctgca gatccttcca
     2821 ttcggtcgag cgatccgtct tccagaccgt ctctccgttg tttttgttga gtgcggcgac
     2881 gtattgctgg tctatcccgt caaacgtcag gataatcaga tcgccgtaga gcagaggcga
     2941 cgaccccggg ccccggtaat ggcggcaggg gaggtcgcgg cgcgtccaga tcggtttggc
     3001 cgtggcggtg tccaggcacg cggtgccata cgacccgaaa tggacgtaga cccggcccgg
     3061 ttcgatgacg ggcgaaggcg acgcgtaggt attgatgttg ttgcccagcg gctccgggtt
     3121 ttcggtctca aaaatggtct cattgaagag gaattcaccg gtttcggcgt caacacagag
     3181 ggcgaacgac tggcgcccgt cctccgtagc actggtcagc caaatctggc cgttgagaat
     3241 caccggcgtc gacaatccct cgtgggggat cgccgttttc caggcgacgt tccccgtttc
     3301 gctccaggtc aacggcagcg ccgcgggttc agtgctgccg ggcgcctgca catgaccgtc
     3361 atgccacggc ccccggaaat ccggccagtc cgtcgctgcc cgggccaaaa atgcgggcag
     3421 aaacgccaga gacaaagctg ccgccagcaa cacgcgaacg gcaaaacaac gccggaattc
     3481 caacataaaa cacatctcct ggtgagaggc tcggggacac gcagccaacg cgcgccattg
     3541 tacgcaatgg cggcatgagg atcatcctca cggagctggt tctttcgcgg cggtgtttcg
     3601 gacctcgtag atctcgacct gttcgaggcc cgtgccatcc agcgcaggca ctttcaacac
     3661 ctggatcagc gcgaacacct cactctggcg gccccgccat tgatccagca gctcaacggc
     3721 gccctccgga caggcgtcgc ggtgtgcgcg gacataggtc tccggaaaca ccaggtacgc
     3781 gatcccgcgc tgcgccagcc aatcgcgcaa cgtggccgga tccgccccgt tcaagggggc
     3841 cagcgcccgg agatttcgga atgcctgcga gtcgaagaag taatgatagg gttcgggcgt
     3901 gagaatgcgg cgtccgcccg gcaggttgtt gttcgcccat tcgatggctt catagcgagg
     3961 gacccgctgt gccagaaatc ctgcgcgtcc ggcgccgtgc agggccgcgg cggcggtcca
     4021 tccgttgatg cacagaccct ggagcaagac cgctgtcaat gccacaaaaa cgagcggccg
     4081 cagcgcagtc agccggcagg cccccaccgc cgcggcaacc acaaaaatgc tggtcagtgg
     4141 gagaaaagcc acgtgagaac ggtcgacgaa atacagtgcc aggccacaga ccgttgcgaa
     4201 cccgcacagc cagcgcacgc gtttgcctcc aaccaccagg cccggcagcc cgaagatcag
     4261 caggagcccg ctgggcgccg tggtccagcc gccgtaccag tgcggacgga gtatcaggtt
     4321 ccagggaaat ttgagccagg ccagcaggcc ggtctcagcg gcatgctggg tttcagaggc
     4381 gacaggaagg atgaaaggcg cgcccgcgag cgcgcgggtc acaacccacg gggccagcga
     4441 cgcagccaga gcgctcgcga acacaaggct ctgctttcca cggttgtctt tggccagaca
     4501 tgcgacgaaa agcggcgccg cgcagcacag aatcagggcg gtccaacgcc cgtcccacgc
     4561 gcttcccagg aaaatcgccg ccagcaccag ccagacgccg gcccgctccg cccgccagat
     4621 cagaagagca gacagggccg ccagtatcag ccccaatcca aggaggtcgc ccgagaccgc
     4681 ggccacacgg ttcaggaagg cgggcgttgc ggcaaaggcg cccgcggcca cggcccccgc
     4741 gcacggtcct gacaggcggc atgcgagcac atggatggac agtatcgcaa agacagccgt
     4801 cagccaggac aaaacccggg cgcctccttc gccgccggac gcatacgccc acgtgtagag
     4861 gcattggacc agggtggcgt aggcaggcgt ttccgcgcct tcgtacggaa ccatgcggtg
     4921 ctggaagtca cagtctttgg cggccgccaa cgcccctgag acggtctcga ggctggttgc
     4981 ggggacgcag gctggaacag ccgccaggac catccccgaa gccgccgcgg cggctcccag
     5041 ccagaacagc gccgcgctgc ttttccgcgc cggaggctca gtaaggcttg cgtcaacgga
     5101 atcgcgttct ttgcgccgga agacttcgca ggtgagtccg cccacggcga tcgcgatgag
     5161 cgcccagcgg gccaatgcaa ggctgatccc tcccgccagc aacacaatcc caccgcagag
     5221 cgccagaccg gtacagagac gcgccaactg gcctgccgca ccacgtttaa tcccgaacgg
     5281 atgcagtgcc cacgagccga gagcgagcgc ggctacgggt aagacaagaa agaccataca
     5341 ctcctcgcat acgcgccccc gcgggcacag accactcggc gcaacatacc acgcccctgc
     5401 aattaaagaa aagcgggcga cgcgcgccgt ttacgatcgt atcatggggt attgtcgcgc
     5461 cattcacttt gttacgaggc aaggctggca ggcctttaac cggcgataaa cccccatgcc
     5521 tggtaagagc attgatctca cggcgatgaa tccctcagta cgattaccaa ctggcctgac
     5581 gcgtttttcc ggcccgcccg gtttaccgcc agccgccgcg gtggtaagat gctgcagtct
     5641 cccggggcaa ctccgggata cggcgctagt gcacgatcag aaaccgaaag agggactgtt
     5701 atgagcacga tacccaggcc cgcgcccgtg cagggcgaca tacagtggtt cgtcgaatcc
     5761 cggttcggaa tgttcatcca ttggggcctg tatgcgctgc ccgcccggca tgagtggatc
     5821 aggcacggcg aggaaattcc cgacgacgcc tatgcaccgt acttcaagtt ttttgaaccc
     5881 gacctctttg acccgggcgc gtgggcacga atggcccgcg aagcaggcat gaagtatgtg
     5941 gtcatcacga ccaagcacca cgaaggattt tgcctctttg actcgcaata cacggactac
     6001 aaggctccca ataccccgtg cagaaaagac ctgatcgaag aggtcgtgga cgcgtttcgc
     6061 gccgaagggc tgcgaattgg attttattat tcgctgatcg actggcatca ccccgatttt
     6121 ccgatcgacg ccatacatcc gctgcgcaac caccccgatg cgcatgacct gaaccgctcg
     6181 cgcgacatgg ccagataccg cgagtacatg ttccaccagg tgcgggaact gctcacgaac
     6241 tatggaaaaa tcgatatcat gtggttcgac ttttcttatc ccaacgagag actcaatggc
     6301 ctcggaggca aaggccggga cgactggcac agcgaggcgc tgctgaaaat ggtgcgcgag
     6361 ttgcagcccc atgtgatcgt cgacaaccgg ctcgatctgc cgcccgacat ggccgatatc
     6421 cataccccgg aacaatttca gccccgcgaa tgggtcacgg ttgacggcaa acccgtgtat
     6481 tgggaggcct gccagacctt cagcggttcc tggggatact atcgcgacga agacagctgg
     6541 aaaagccccg aacagctcat tcagatgctg atcaacacgg tgagctgcgg cggcaacctc
     6601 ctcatgaacg tcggcccgac cgcgcgaggt ctgttcgact cccgcgcccg gaacgcgctg
     6661 aaggtgtacc gcgactggat ggccctgcac agccgttcga tttacggctg cacccagagc
     6721 gaattcgccg cgcccgaaca cggccgcctc acgcagaacg gaaaacggct ctacgtgcac
     6781 ctctttgcct ggccgttcaa gcatctccat ctcgacgggc tggcggggaa ggtcgaatac
     6841 gcccagcttc ttaacgacgc ctcggagctc cgcatacaca agaacgccgg cagcacggat
     6901 gacggccgcg cgacgctgac cctcgacttg ccggtcaaaa agcctgaggt ggtggtccct
     6961 gtggtcgaac tgtttctcaa atgaatatta accgctgagg aaatcgtctg aatatggatg
     7021 agaataccgt tattctggat gccatcccgt ttgaagtgga cgaagccggc gcgctggcgg
     7081 ccctgcagct gacgcccgat tctccgttcg cccaggaggc ggtggccctg ctgcacgaag
     7141 ccgcttccgt ggcgcggccg aagggcatgt accgcctttc agcggtcgag accatcgaca
     7201 gcaacacgac ggctctggat ggcgttcagt tcacgagccg cattctgcgc ataaatctca
     7261 cgggtttgtc ccgcgcgttt ccgtttgtcg ccacgtgcgg cgttgagctc gaggaatggt
     7321 cgcgttccaa gaccgacgtg atgcaccagt tctgggcgga tggcctcaaa gaacgggccc
     7381 tccagagcgc catcggcgcg ctggaaactc atctggccgc ccggtatcaa tcgggaacgc
     7441 gcggcatgat gaatcccggt tcgctggttg actggccgtt gtccgagcag ccccggcttt
     7501 tttctctctt cggcgacgcg tgcggacgga ctggtgtgcg gctcagtgag agttgtctca
     7561 tgatccccgt aaaatcggtg tcagggatat ggtttgagac cgagacggaa ttcgtcaatt
     7621 gccagctctg cccccgggaa gactgcccca agcgcaggac ccccttcgac gcccgtgccc
     7681 tggagcatta cgcgtgagcc gggccttcat cgcggcgtga aagatctccc caggatgtca
     7741 agcgccagca ccgggcacgc cccccgggct tttccggcat gtaccgcgtt tgaaatgcgg
     7801 gacgggcata tgataagctc ttttcgctca acgaagtgcc tacccgtagc tcaattggat
     7861 agagtgcttg gctacggacc aagaggttgg gggttcgagt ccctccgggt aggccatgca
     7921 gattcgcgcg caacaccagg ctcccccggg gttgcgcgct tttacttttg ccactcggca
     7981 ctgtgcgttt ttcgcgccga aacgcgggtt gaggtaccat agactactga agaccactga
     8041 ggcgcctcag cggcgcggat atccacgctc cacaaggaaa cgggcaatgg actgggcaga
     8101 attggcggaa tggattcacc gggtgaagga atcggacctg ccggcgccgc ctgtcccgct
     8161 ggccccggcg atccaaatcc gcgatgtcag ccggtatctc gcggctctgc gaaacgacgt
     8221 tgccaaagga gccgatagcg cccgcgcccg cacaggggcg ctgcagcgcg agatcaaacg
     8281 gcttatggac atcgcgcggg aagtgacggc cccgccccgg gaatcgcccg cagtggctgc
     8341 gccggccgaa ccgccggccg aggccggctt acccgtttct cccgccggac ctgtgatcgt
     8401 ggcattcgat ctcgagacca cgggcctgga tccgaacgaa aacgacatta tcgaggtggg
     8461 cggcgtgaaa ttcacaccgc agggcgacgt gctgggccgt tttgagcagt tcgcaaaccc
     8521 ggggtatgcg atcccggcgg agagtacggc ggtctcgggg atcacgaccg agatggtggc
     8581 cagtgcggac ccgtcgtttg acgtgctcaa ggcgtttatc gagtgggcgg ggccggatgc
     8641 cgtgtatgcc gcgcacaacg ccgcctttga cgcgggattc atcgaagcca cctatgaaaa
     8701 ggccgggagg aagtgcccgc cgctgaaggt cgcggacacg cttgagtggg cgcgctcgct
     8761 gaagctggag acgcccaatt gcaagctggg caccctgctg gctcattacc ataaga
//
LOCUS       DAOPBQ010000071         8727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7555_length_8727_cov_4.356204, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000071 DAOPBQ010000000
VERSION     DAOPBQ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7555_length_8727_cov_4.356204"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..2837)
                     /locus_tag="PKW60_03700"
     CDS             complement(153..2837)
                     /locus_tag="PKW60_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPJ98565.1"
                     /translation="MCARLFAALLVLILSASPVFSEEGAGRAVYMRWEELPDLPEPMG
                     GQFAGMHCSGSGESALLVAGGTRWEGSFLDGGTKHWLDRIYVYTLPAGTAKPGGVRGA
                     LHELFRFKAAQPVGELVSAGAWREAGRLPEALSYGGSVSTPKGVVCIGGLHGTDYSRN
                     VLLLQWNGREIIQSELPPLPEPMALGCAASLGTVIYAGAGQTGPGHDSKSFWRLDLDR
                     AHAGWEALEPFPGPARILPVAAAQDGSLFVFSGAALRDDGSGRSYLTDAYRYTPRQGW
                     SSLAPSPRPIAAAPALAWGDTHILVFSGDDGLLVDQIAELGDAHPGYPLDVLGYHTVT
                     GTWAYLGAIPDAPLTSPAVPFQGGIVVAGGEPRPGQRVSRVLWGIPERTQRAFGWLDY
                     AVVGVYFVILAAMGLYFSGRENSTEDYFLGGRRVPWWAAGISIYGTLLSAITFLATPA
                     TSFKGDWAYSVGNVLMVAVAPIVIYFYLPFFRRLDVMSAYEYLEKRFNLGVRMFGSTA
                     FTIFQLGRMGIVLLLPALALSAVTGIDLRLSILAMGVLCTFYTVMGGIEAVIWTDVVQ
                     VVILLGGACLSFVLVSAAVDGGFVGMLETAYADGKFHMVDWGWDITQKVFIVVVIGRF
                     LEALIPYTTDQTVIQRYLTTSSEKQAARSIWTGALFSLPSFVIFFGIGTALYVFYKEH
                     PGQLDPNLTTDQIFPLFIVQQMPAGVGGLVVAAVFAAAMSSLDSSMNSVTAVLVTDFY
                     QRLSRGGNQKTAMLLARTLTLALGAFATVVALYMAVSGITGLWEKYMSIIGLFGGSLA
                     GVFALGIFTRRAHGAGALAGAVAGACVLYCVQRYTSVSFLLYSTTGILASFGIGYAVS
                     LVLPASPKDITGLTWATLASRPPEDGGRTAPVRNGNRS"
     gene            3198..6101
                     /locus_tag="PKW60_03705"
     CDS             3198..6101
                     /locus_tag="PKW60_03705"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPJ98566.1"
                     /translation="MAIWIAVAAAIIAAYFLTSRKEPPPKPSTISYSERPSDRLNQSR
                     LENRSRYGSPRTIDPPRRSGENSEPEDPLALIGVISEASTNQPLEGAFVRSMMLPPQD
                     ESSLSETDGQHEAETARMTPAFLDKAQGARRSTKTNDKGQYALPIPMPGRYLIQVARA
                     GFVSRREVVEFVEGGEKQVRLDLALSKGASIAGRVLEAGTNAGIPLILITAHNEEGTS
                     SAETETAPDGTYELTGLVPGTYQVALDLTRQPYKASGRIPVQNVVIQHPDEKRTDINF
                     TLSPAGTVWGYITDHEKQPVKGTQVYLCTSESLISQVLNAAVHQMPPLNSRSEKDGYY
                     ELVGVPLNQEWRVYATTDEYAPQLTDPFIVTESARNVRVDIHVLRGSSVYGRVLDQVG
                     TPIPNADTVCAPSYSKLFQRLDEARALRHTETEADGSYVIANLPPGDYQILAQKEGYK
                     IALMGDPLYADGFNDIRGFDLVLMAVDSGRYVIYGKVVDAGGRPIPGVNLTLSGLGTE
                     SMSATEMETRSDERGEFGFYGIESGFLVILAEKEGYTSQQVSEVRLDEPTTIVMQAAG
                     LVRGRVLVRETGQPPDRYQVRAVPSDSLQSGIGLALMSSGDPRDLRSFNNADGSFELA
                     LAPGDYTIEATAHGLTPGREAVSVLEGQSIDGVTVYVSQSGGAIQGQVQTMEGRVPAG
                     ATVWLGGAAEAQFGRLVDMVAQTQRHGVQVGADGRFEFRNLADGTYTVLAQAEGYAQG
                     SSGPVNVAQSRTVTGVVVMLGGGGRLQGYVARNGVSLPGAMVTVIGNGISEMTSADQN
                     GQYAIDGLAAGTYLATAVSFEGRGTDLFAPMHAQVEIREGEVTVHNFGEEGGASVQGL
                     CTPPPASGVFGFALLRAPGSAAALTGLNLTNPADWYSGMGAGGAPGILGMSPIRNDGY
                     FKIDNCPEGDYQLDILYVNLGEAMSGTGRPRTSHAVSIRGDEVIELDIHVPGS"
     gene            6109..7023
                     /locus_tag="PKW60_03710"
     CDS             6109..7023
                     /locus_tag="PKW60_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98567.1"
                     /translation="MSVVELNNVEVSYGATKALRGVTCTVQGGAVGLLGPNGAGKSTL
                     LKTLLGFVRADAGEVSLFGFRLPGQALEARQRLGYMPERDVVGRKVSAVSFLTYCGCL
                     FGMKRTDAMERAHEVLNYVGIGDSRYRKMQTYSTGMSQRIKFAQALVHDPKLLLLDEP
                     TNGLDPEGRIEMLELIKELVTKRGVTVLLSSHLLPDVEHVCDHVLIIHEGRIVREGSI
                     GDLTSARENLFEIRVREPQDPYRIALEAAGCAPKILPNGNLLVEKPAQMDLRAFFELA
                     EVQNTHVRHFTPVRHRLDEVFMQAIGRH"
     gene            7024..7899
                     /locus_tag="PKW60_03715"
     CDS             7024..7899
                     /locus_tag="PKW60_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPJ98568.1"
                     /translation="MPVYTQTYRAYDGKPLQGFRWWVMVKQELRVLFGSRAFVYLLVM
                     AVIHTSLRVLQITVYDMMSTSSQTPALMALRNIAMMQVDERTFFDYLRIQSPLVFIIM
                     LFAGSGMICNDFRDNLIEIYFSKPLTRLDYIAGKVITLVLVGALLTAIPDTLLVLLHN
                     LLSPGWETLRETYWIPFSIAGFSACIVLPCALGVLASSALFSSERYAGIAVFMILFAN
                     SALGTVLPALVRNPNFSIVSLPMAVNRVGETLFAQPRKSFSLHWGWPALVIVLLCAFC
                     SWAIASRIRRAERAT"
     gene            7896..>8727
                     /locus_tag="PKW60_03720"
     CDS             7896..>8727
                     /locus_tag="PKW60_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98569.1"
                     /translation="MSAIIEAQGLSKWFGEVVAVNNLNVSIAPGVTGLLGPNGAGKST
                     FIKLALGLYSPSRGSITIFGEPPRNNLEVLRRIAYCPEFDRFPENTTGFEFVYWLNRF
                     YGMTDPQATKRAEEACERVKMTDRMDDLIETYSRGMRQRIKIAQALAPSPDLLFLDEP
                     MAGLDPEGREEMFSLIRELGEDGRTVLVSSHILYEIERVTSHVVLLHNGCILARGKVN
                     DVRELLDEHPLAVTVECAAPRDLARHFAEDSATLSIEFADGRMTVRTRNANGFFEKLN
                     R"
BASE COUNT         1813 a         2768 c         2640 g         1506 t
ORIGIN      
        1 atcggccgtg tcgaagtaat tgacccccgc atcaaaggcg agccgcacca tctcgcgcga
       61 catggcctcg tcgcagcggt cgccgagatt catgtcggta cccatgccca gggcacttat
      121 gcgcaccccc catttgccca gttgtctata cttcatgagc ggtttccatt gcgcacgggc
      181 gcggtacggc ctccgtcttc cggcgggcgg cttgccaggg tagcccaggt gaggccggtg
      241 atgtcttttg gagaagcggg caataccagg ctgacggcat acccgatgcc gaagctcgcg
      301 aggatccccg tggtcgaata caacagaaaa ctcacgctgg tataccgctg aacgcaatac
      361 aggacacagg cgccggccac tgcgcccgcg agcgcgcctg ctccgtgggc ccggcgcgtg
      421 aagatgccca gcgcgaatac gcccgccagg cttccgccga acaacccgat gatgctcatg
      481 tatttttccc agagccccgt gatcccgctc acagccatat aaagcgcgac gacggtggca
      541 aacgcaccca gcgccagcgt caatgtccgg gcgagaagca tggccgtctt ttgattgcct
      601 ccgcgactca agcgctggta aaagtccgtc accagtacgg cggttacgct gttcatgctg
      661 ctgtccagcg acgacatggc cgccgcgaac acggccgcga cgacgaggcc gccaaccccc
      721 gccggcatct gctgcacaat gaaaagcggg aaaatctggt ctgtggtcag gttgggatcc
      781 agctggccgg gatgctcctt ataaaacaca tagagcgcgg taccaatgcc gaaaaagata
      841 acgaagctcg gcagactgaa cagggccccg gtccagatgg agcgggccgc ctgtttctcc
      901 gacgacgtgg tcaggtagcg ctgaatcacg gtctggtcgg tggtgtacgg gatcaaggcc
      961 tcgagaaagc gtccaataac caccacgatg aacaccttct gcgtgatatc ccagccccag
     1021 tccaccatat ggaacttccc gtcggcatag gcggtctcga gcatgcctac gaatccgccg
     1081 tccaccgcgg cgctgaccag cacaaaagac aggcaggctc ccccgagcag gatcaccacc
     1141 tgcacgacat cggtccagat aaccgcttcg ataccgccca tcacggtata aaacgtacac
     1201 agcacgccca tggccagaat ggacagccgg agatcgatcc ccgtcacggc cgacagggcc
     1261 agtgcgggca gcagcagcac gatccccatc cggccgagct gaaagatggt gaaggccgtg
     1321 ctgccgaaca ttcgcacccc cagattgaac cgtttctcga gatattcgta ggccgacatc
     1381 acatcgaggc gccggaaaaa cggaagatag aagtaaatca cgatcggagc gaccgccacc
     1441 atcagcacat tgccgaccga atacgcccaa tcgcctttaa atgacgtggc tggcgtcgcc
     1501 aggaacgtaa tggcgctcaa cagcgtgcca tagatgctga tccccgccgc ccaccagggc
     1561 acccggcggc cgccaagaaa gtagtcttcc gtgctgttct cgcggcctga aaaatacagg
     1621 cccatggccg cgagaatcac gaaatagact ccaacgacag cgtagtcaag ccacccgaac
     1681 gcccgctgcg tgcgctcggg aatgccccac aggacgcgcg acacacgctg ccccggcctg
     1741 ggttcgccgc cggcgaccac gattccaccc tgaaacggca ccgcgggaga cgtgagcggg
     1801 gcatcgggga tcgcgccgag atacgcccag gtaccggtca ccgtgtggta gccgagcaca
     1861 tccagcggat accccggatg cgcgtcgccc agctcagcga tctggtcgac cagcagcccg
     1921 tcatcaccgc tgaacaccag gatatgcgtg tcgccccagg ccagcgcggg cgccgctgca
     1981 atcgggcgcg gggacggggc cagggaactc catccctgcc tcggtgtata gcggtaagca
     2041 tctgtaaggt agctgcggcc ggagccgtca tcgcgcagtg ccgctcctga aaacacgaat
     2101 aaggacccat cctgtgctgc ggccacgggc agaatgcgcg ccggcccggg aaacggctcc
     2161 agcgcttccc atccggcatg cgcccggtcc aggtcgagcc gccagaatga tttcgaatcg
     2221 tgtcccggtc cagtctgccc cgctccggcg tagatcaccg tgccgagtga cgcggcacat
     2281 cccagcgcca taggttcagg cagcggcggc aattccgatt ggatgatctc gcggccgttc
     2341 cactgcaaca agaggacgtt ccgggaatag tctgtgccgt gcaggccgcc gatgcagacg
     2401 actcccttgg gggtggagac cgaaccgcca taggagagcg cttcggggag ccgcccggcc
     2461 tcgcgccagg cgcctgccga tacaagttcg ccaactggct gagccgcctt gaaccggaac
     2521 aactcgtgca gagcgcctct aacacctccc ggtttggcgg ttcccgcggg cagtgtgtat
     2581 acgtagatgc ggtcgagcca gtgtttcgtg cccccgtcga gaaacgagcc ttcccagcgg
     2641 gtgccgccgg ccacaagcaa tgccgattcg cctgagccgg agcaatgcat ccccgcgaac
     2701 tggcctccca tgggctcggg gagatcagga agctcttccc agcgcatgta gacagcgcgc
     2761 cctgcccctt cttcggaaaa aaccggggaa gcggagagaa tcaggaccag caatgccgca
     2821 aatagccgag cacacacgaa caaaacatcc cccaacagat ccgcacgtga atgaagacgc
     2881 agcctaacag acacgcagaa accagatcaa acactggatg caatttagac tgcgctgaag
     2941 cccggacagt gatcttgcgt tttataggcg gctctactaa gggttcaggc gcggaagttt
     3001 gtgaatatcc ccggctattg gtatcatctg tagcttgaaa gcgggggtaa aacgcccacg
     3061 cggcgcagaa catccaacgt gacggatgcg gcagtttttt gagcccgtgg aaccagagaa
     3121 ccgtcgctgt tgacgcacag gaagccgtgg cccgaagtca taactcacca gagcgattca
     3181 gcccgtttca gaaaatcgtg gccatttgga ttgccgtcgc ggcggcaatt atcgccgcct
     3241 attttctgac ctcgcgtaaa gagccgccgc cgaaaccgtc gaccatttcc tacagcgaac
     3301 ggcccagcga tcgcctcaat cagtcccgtc tggagaaccg atcccgctac ggatcgccac
     3361 gaaccattga cccgccccgg cgcagcggcg agaactccga accggaagat ccgctggctc
     3421 ttatcggcgt tatcagcgaa gcaagtacga accagcctct cgaaggcgca tttgtgcgct
     3481 cgatgatgct gcccccgcag gacgaaagct cgttatccga aacggatgga caacacgaag
     3541 cagagacggc ccggatgacg ccggcctttc tcgacaaagc ccagggagcc cgccgaagca
     3601 caaaaacgaa cgacaagggc caatatgcac tccccatccc aatgcccggc cgttacctca
     3661 ttcaagtggc tcgggccggt tttgtcagcc ggcgggaagt cgtggagttc gtggagggcg
     3721 gcgagaaaca ggtccggctc gatctcgcgc tttcgaaagg cgcgtcgatc gctggccggg
     3781 tgctcgaagc cggaaccaac gcgggcatcc ccttgatcct gatcaccgcc cacaatgagg
     3841 aaggcacgtc cagcgcggag acggagacag cgccagatgg cacctacgag ctcacgggac
     3901 tggtgccggg gacctaccag gtggcgctgg atctgacgcg ccagccgtac aaagcctccg
     3961 ggcggatccc cgtacaaaat gtggtgatcc agcatccgga tgaaaaacgg accgacatca
     4021 atttcaccct ttctcccgcg ggtaccgtct ggggatacat cacggaccac gaaaaacagc
     4081 ccgtgaaggg cacccaggtg tatttgtgca cctccgaaag cctcatctcc caggttctca
     4141 acgccgccgt acaccagatg ccgcctctga acagccgttc cgaaaaagat gggtactatg
     4201 agctggtcgg cgtgccgctc aatcaagagt ggcgtgtcta tgccacaacc gacgagtacg
     4261 cgccgcaact cacggacccg ttcatcgtga cagaatcggc acgaaatgtc cgggtcgaca
     4321 ttcatgtcct gcgcggctca tccgtatacg ggcgcgtgct cgaccaggtc ggcacgccca
     4381 tccccaatgc cgacaccgtc tgcgctccat cgtattcaaa attgttccag cggctggacg
     4441 aggcgcgcgc gctccggcac accgaaacag aagccgatgg cagctatgta attgcgaacc
     4501 ttcctccggg agactaccag atcctggcgc aaaaggaggg ctataagatc gcgctcatgg
     4561 gcgacccgct gtacgcggac ggcttcaacg acatccgagg ctttgacctg gtgctgatgg
     4621 ccgtggacag cggccgctac gtcatatatg ggaaggtcgt ggatgccgga ggacgcccca
     4681 tcccgggcgt caatctgacc ttgtccggat tgggaaccga atccatgagc gccaccgaga
     4741 tggaaacccg atccgatgag cgcggagagt tcggattcta tggcatcgaa tcggggttcc
     4801 tggtcattct tgccgaaaag gaaggctaca cctcgcagca agtcagcgag gtacgcctcg
     4861 acgaaccgac gacgatcgtg atgcaggccg cgggattggt gcgcggcagg gtgctggtgc
     4921 gggagaccgg gcagcccccc gaccggtacc aggtgcgggc ggttccatcg gattcgctgc
     4981 aaagcggcat cgggctggcg ctgatgtcca gcggcgatcc acgcgatttg cgcagcttca
     5041 ataatgccga cggcagtttc gagctggccc tggcaccggg ggattacacc attgaagcca
     5101 ccgcacacgg attgacaccc gggcgcgagg ccgtctcggt cctggaagga cagagcatcg
     5161 acggcgtgac cgtgtatgtc agccaatccg gcggcgccat tcagggacag gtgcagacca
     5221 tggaaggcag ggtcccggct ggcgccaccg tatggctcgg aggcgcggcg gaggcccagt
     5281 tcgggcggct cgtcgacatg gtcgcccaga cgcaacgcca cggcgtgcag gtcggcgccg
     5341 acggacgttt cgagttccgc aatctcgccg atgggaccta tacggtactc gcccaggcgg
     5401 aaggctacgc acagggcagc agcggccccg tgaatgttgc acagagccgg accgttacgg
     5461 gagtcgtggt catgttgggc ggcggcggac gcttgcaggg ctatgtcgcc cggaatggcg
     5521 tctccctgcc cggagccatg gtcacggtga tcggcaacgg catcagcgag atgacatccg
     5581 cggaccagaa cggccaatat gctatcgacg gcctggcggc cggcacgtat ctcgcgacgg
     5641 cggtctcttt cgaagggcgc ggcacggatc tgttcgcgcc catgcacgcc caggtggaga
     5701 tccgggaagg cgaggtcacc gtacacaact tcggggagga aggcggcgcc tcggtgcagg
     5761 gattgtgcac accgccgccc gccagcggag tcttcgggtt tgcgctcttg cgcgcacccg
     5821 gcagcgcggc cgcgttgacc ggcctgaacc tcacaaatcc ggccgactgg tacagcggga
     5881 tgggtgcggg cggcgcgccc ggaattctcg gcatgtcgcc catacgcaac gacggctact
     5941 ttaaaattga caactgtccc gagggggact atcagctcga catcctgtac gtgaacctcg
     6001 gagaggcgat gtcgggcacg gggaggcccc gcaccagcca cgcggtgtcg atacgcggcg
     6061 atgaggtcat cgaattagac atccacgtcc cgggatcgta aggaaatcat gagcgttgtt
     6121 gaacttaata acgtcgaagt ctcgtacggc gcgaccaaag cgctccgcgg cgtgacctgc
     6181 accgtgcagg gcggcgcggt cggcctcctc ggcccgaacg gcgccggcaa gagcaccttg
     6241 ttgaagaccc tgctcggctt cgttcgcgcc gatgcgggcg aggtgtcgct gttcgggttc
     6301 cgtctcccgg ggcaggcgct cgaagcccgg caacggctgg gctacatgcc ggaacgggat
     6361 gtggtgggcc gaaaagtgag cgccgtgtcg ttcctgacct actgcggatg ccttttcggc
     6421 atgaaacgta ccgacgccat ggagcgcgcc catgaggtgc tcaactacgt cggcataggc
     6481 gacagccggt accgaaagat gcagacctac tcgacgggga tgtcccagcg gatcaaattc
     6541 gcccaggccc tcgtccacga tccgaaactc ctgctcctcg atgagcccac caacggcctc
     6601 gatcccgagg gccgcatcga aatgctcgag ctgatcaaag aactggtgac gaaacgcggt
     6661 gtgacggtgc tgctctcctc gcaccttcta ccggacgtcg agcatgtatg cgaccacgtg
     6721 ctgatcattc acgaggggcg gatcgtgcgg gaaggctcca tcggcgacct gacctccgcg
     6781 cgcgaaaatc tgtttgagat tcgggtgcgc gaaccgcagg atccctaccg gattgccctg
     6841 gaagctgccg gttgcgcgcc taaaatcttg cccaatggca atctcctggt cgagaaaccg
     6901 gcacagatgg acctccgggc cttttttgag ctggccgagg tccagaacac acacgtgcgc
     6961 cattttactc cggtccggca tcgtctggac gaggtcttca tgcaggcgat agggaggcat
     7021 tgagtgccag tctatacaca gacataccgg gcgtacgacg gcaaaccgct gcaggggttc
     7081 cggtggtggg tcatggtaaa acaggaactg cgcgtcctgt tcgggtcgcg tgcgttcgtc
     7141 tatctgctcg tcatggcagt catccacacg agtctccgcg ttcttcagat aaccgtgtat
     7201 gacatgatga gcacatccag ccagactccg gccctgatgg ccctgcgaaa catcgccatg
     7261 atgcaggttg atgagcggac gttctttgac tacctgcgga ttcaaagccc gctcgtgttc
     7321 atcatcatgc ttttcgcggg ctcgggcatg atctgcaacg atttccgcga caacctcatc
     7381 gagatctact tttccaaacc gctcacccgc ctggactaca tcgcgggcaa agtcatcacg
     7441 ctggtcttag tgggcgccct gctgaccgcg ataccggaca cgctgctcgt gctgcttcac
     7501 aacctcctga gccccggctg ggagactctg cgcgaaacgt attggattcc gttttcgatc
     7561 gccggattca gcgcgtgcat tgtgctgccg tgcgcgctgg gcgtgctcgc cagctccgct
     7621 ctcttctcca gcgaacgcta cgcgggcatc gcggtgttca tgatcctgtt cgccaactcc
     7681 gccctgggca ccgtgctgcc ggcactcgtg cgcaacccca acttttccat cgtctcgctt
     7741 cccatggcgg tcaaccgcgt gggagaaacg ctttttgcgc agccccgaaa gagtttctcg
     7801 ctgcactggg gatggccggc actggttatc gtgttgctct gcgccttctg cagctgggcg
     7861 attgcatcgc gcatccgtcg cgcggagcgt gcgacatgag cgcgatcatc gaagcccagg
     7921 gcctgtccaa atggttcggc gaggtggtgg ccgtcaacaa cctcaatgtg tccatcgcgc
     7981 cgggagtcac cggcctgctt ggacccaatg gcgcgggaaa aagcaccttc atcaaactgg
     8041 cgctgggcct ctacagtccc agccggggct caataacgat cttcggcgag ccgccgcgca
     8101 acaacctcga ggtgctgcgg cgcatcgcct attgccccga atttgaccgc ttcccggaga
     8161 atacaaccgg cttcgagttt gtctactggc tgaaccgctt ctacggcatg accgacccgc
     8221 aggccaccaa acgggccgag gaagcctgtg aacgcgtcaa gatgaccgat cgcatggacg
     8281 acctgatcga gacctacagc cgcggcatgc gtcagcgcat caagatcgcc caggccctgg
     8341 cgccatcccc cgacctcttg tttctggatg agccgatggc gggtctggat cccgaaggcc
     8401 gcgaggaaat gttctcatta atccgggaac tcggagaaga cgggcggacc gtgctggtat
     8461 ccagccacat tctctacgag atcgaacggg tcaccagtca cgtggttctc ctgcacaacg
     8521 gatgcattct cgcgcgcggg aaggtgaacg acgtgcggga gctgcttgat gagcaccccc
     8581 tggccgtcac ggtggaatgt gctgcgccac gggatctggc acgccatttc gcggaagaca
     8641 gcgcgacgtt gagcatcgaa ttcgcagacg ggcgcatgac cgtgcgaacc cgaaatgcca
     8701 acggcttttt cgagaaactg aaccggc
//
LOCUS       DAOPBQ010000072         8669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7605_length_8669_cov_3.493035, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000072 DAOPBQ010000000
VERSION     DAOPBQ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8669
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7605_length_8669_cov_3.493035"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKW60_03725"
     CDS             complement(<1..548)
                     /locus_tag="PKW60_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPJ98570.1"
                     /translation="MAQALCETQLPGIEPVARGKVRDIYDAGDALLIVATDRLSAFDW
                     VNPVGIPDKGKILTQISLYWFDATRDLCRNHLISSDIRDFPREFQGYPDQLEGRSMLV
                     HKCTMFPVEFVVRGYLSGSGWKEYREHGTVCGIPLPKGLKESEKLDAPLFTPATKASS
                     GHDINISPQEAAEIIGADWARAA"
     assembly_gap    709..718
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            965..1759
                     /locus_tag="PKW60_03730"
     CDS             965..1759
                     /locus_tag="PKW60_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98571.1"
                     /translation="MSLLEQLGIPWILSGLLLIPYLCWGVYILRERFHHQAELSVACE
                     AITFLGLVVFFVIEFAMLSMWLSRSPVLFLLAVFGLLASLLALYGPMLMSLGSHMLVG
                     AVMPSGAPLSHTPKYGAAEGFEQKGDYEAAAREYMAVARMFPKEAKASLRAGDNLIKL
                     DRVEEGVVWLKRGLHYLTEPEESLRVVNRIAEIYHRRLDRPDEAKEAFRDYLSRFPDA
                     EYADSVKRRLARLGGPETPNNAEKTSPRPGDPPILVSEGPSYLPDD"
     gene            complement(1772..2212)
                     /locus_tag="PKW60_03735"
     CDS             complement(1772..2212)
                     /locus_tag="PKW60_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPJ98572.1"
                     /translation="MSSAFGGRSRRRPTINITSLIDVMFLLLIFFMVSSTFREHLGID
                     ITLPQAESAEEQRLDIHEVTVTAKGDLYFGEQRVDEIGLKESIAALLTAEPEAVLVLR
                     ADEGADFGRVLRAIDITRSAGGARMIIPTRYRDVAQPEPGASSP"
     gene            2540..3469
                     /locus_tag="PKW60_03740"
     CDS             2540..3469
                     /locus_tag="PKW60_03740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPJ98573.1"
                     /translation="MHSCCICVADSDEASARLLADGLKLNEYEVRVATTPEDVCRECS
                     QGDVQVLLLDAALGDMGGLALARQLKTDHETSGVILLLALDREAQKRFSIEDALGVDD
                     YITKPYNLPMLMIRIETLLRKRRKVECLKTEEEPLGDSSYTDELTGLRNRRYLLERLQ
                     EEVEKAHRYNFPVSCVVFDVSEVEALDDELGTVSLDDLLVELAMAMRNYSRTFDILAR
                     YDGTMFAAVLPHVDRDQAVQYAQKILNEVEDTTFADPCYPSKTRLSAGIVACKNGSAR
                     SAEAVLGEAMQSLLAAQSAKDKRVGLRDLSDGP"
     gene            complement(3499..4779)
                     /gene="purD"
                     /locus_tag="PKW60_03745"
     CDS             complement(3499..4779)
                     /gene="purD"
                     /locus_tag="PKW60_03745"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPJ98574.1"
                     /translation="MNVLIVGSGGREHALAWKIAQSPQVERLFCAPGNPGMAALGRCV
                     DIGVNDFEKLGALARDAGVGLAVVGPEDPLSRGLVDYLTANGIKTFGPVAAAARLEAS
                     KSFAKNVMNRCGIPTARSAEFMCPDEAIGYVRSTGAPVVIKADGLAAGKGVTVARDLD
                     TAVSAIRAAMEDRAFGDAGNRVLVEEYLEGEEASILAFSDGKTVIPMASSQDHKPAFD
                     NDEGPNTGGMGAYSPAPVVTDALQHEIMNTILNPCVQAMAEAGTPYTGVLYAGLMITP
                     DGPKVIEFNCRFGDPEVQVVLPRMKNDLIPILVACCEGTLEHVSLEWEEGACVTVVMA
                     SGGYPGAYQKGKAISGIPEAEQEAGAVVFHAGTAMEGSTLVTNGGRVLNVTATGSDVP
                     AAIERAYAAVRRIHFENAHYRTDIGMKALKRLGG"
     gene            complement(4808..5419)
                     /locus_tag="PKW60_03750"
     CDS             complement(4808..5419)
                     /locus_tag="PKW60_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HPJ98575.1"
                     /translation="MTPIRRAIISCHDKTGLVELAQLLAEFGVELISTAGTLRVLEEA
                     GIPALNMADYTGVEEMMDGRVKSLHAKIHAGLLGIRDNKLHAEQLQAYGYEWIDLVVA
                     NLHPVEALIAQPGAMPEEVIEQIDIGGLSMIRSAAKNFRYVGAVVNPERYSLVMHEMR
                     AHGGALTFEIRHRLAQEAFASAARYDHAIAEFLKNTQLAWNGG"
     gene            complement(5559..6224)
                     /locus_tag="PKW60_03755"
     CDS             complement(5559..6224)
                     /locus_tag="PKW60_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPJ98576.1"
                     /translation="MPTVKFDSVTKVYRGTPVFQGISATLAPNEGLCLSGPAASGKST
                     LLRLVAGLELPSAGSVTVDDVPAAAGELHTRAIGMVFQELALWPHMSVEKHLLFVLHA
                     QEISREKRIERVEHMLALSGLAGKRRERPGSLSLPHQQRLALARALIVNPRLLLLDEP
                     FTYLERAAVQQFTEEILRRRSAEHLTVIIATQGCREAWPLVDRLLMLNEGRCHYGAIQ
                     PPL"
     gene            complement(6211..7725)
                     /locus_tag="PKW60_03760"
     CDS             complement(6211..7725)
                     /locus_tag="PKW60_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98577.1"
                     /translation="MAVFAAVSVAPLAALGWRSLFEGSVWRFETWRAVFAEPRQATIL
                     ANTLAVSLGTCLTSLLAGGGIALSLQYCPRILRAFFTFLLCVPVLIPPCVFSAAWLDA
                     LTRMGILAPQFTAEPGVPFPFRLLSVILVFTLSYYPFVFLCTVAALQRFDRRMLESAR
                     LIADPVQTFFVVRFPLCAPWVLTGVGLVFLLTLTEPAVPALFQVNTCSVEIFTELSAF
                     HETGRAVAQALPLLAAGSAILWAWGRWVRPRQQWLASTPKPAGRVPVGWKRALLASGA
                     CLFLAALAVGVPLSLLAGRIESWAQLMLAWSDARREILTSLLQSATAATVLTALGFAM
                     AWFANDSPRLGLLFRLSLIPFLLTGPLLGTGLRALWNHPGPTGFVSRSFLILVLANTA
                     KYLYFAYLSERIALLALSRPRLPAARIHRAAPVHVAVWGIACLLSLQELGAVVMVASP
                     DVMPLPVRVHSLMHLGPSAAVASLCLAMASLLFATGLCTAVFCFACGRVLHAYR"
     gene            complement(7971..>8669)
                     /locus_tag="PKW60_03765"
     CDS             complement(7971..>8669)
                     /locus_tag="PKW60_03765"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPJ98578.1"
                     /translation="LELIAAEGDTLQISPTTITRRVGQYVVKACSREPVLGVLKRTLR
                     RERYRRGWVAGLYLEQLGIGAPRVDGLVEQGRWGIIRASYLIMEYLEGANNVERCMAE
                     MVQDGAPEERLRTFLHDLAQTVNALRDAGAYHRDLSGKNIFTRNGEKFYFIDLDSIVP
                     VKRYSRGMLYANHVQLYDSFCDFLGDDLLDSFIALMLPDEEDYATWVKQVRRGQAERR
                     SRQLAIWRKQGRSL"
BASE COUNT         1857 a         2628 c         2492 g         1682 t
ORIGIN      
        1 gccgctcttg cccagtccgc accgatgatc tctgcggctt cctgcggtga gatattgatg
       61 tcgtgcccgc tgctggcctt ggtggccggt gtgaaaagag gggcgtccaa tttctcggac
      121 tccttcaggc ctttcgggag tgggattccg cacacggttc catgttcccg gtactctttc
      181 cagccgcttc ccgacagata cccccggaca acgaattcga ccgggaacat cgtgcattta
      241 tgcaccaaca tggatcggcc ttcaagttgg tcaggatagc cttgaaattc gcgcggaaaa
      301 tctcgtatgt cactggatat aaggtggtta cggcaaagat cccgagtcgc gtcaaaccaa
      361 tacagcgaga tctgggtcaa gatcttgcct ttgtcgggaa tgcccacagg attgacccag
      421 tcaaacgccg ataaacggtc tgtagccacg atcaacaatg cgtcaccggc gtcgtaaatg
      481 tcgcgaacct tgccgcgcgc tacaggttcg atgccgggaa gctgtgtttc acaaagcgcc
      541 tgtgccataa agtgttgttc ctcaggtgct gtctggccgc caacgggcag tgggttctgg
      601 gaaaaccgtg ccctccctca tgcgaccgga ataatacgtg cgctatgggc agttgtcaat
      661 gcagatcctg gcgcagcatt aaattctttt cgagtttttc cacaccccnn nnnnnnnngc
      721 gcgagtttca ggactcgaat tgcacaccac cacggcgcag aatacgcacg catgagtata
      781 aagcccccgt ctggggtaat ttgttgacaa aatcgctgag ataaaggaaa tgttagacaa
      841 ctcagaggta gacgtggtat aaaagagagg aagtcctgcg ggttatgcgg ggccaaggca
      901 ttaattgagc aaccagtagc catctcgcca aaaacgggct gcgggcagtg agggaacacg
      961 gcacatgtca cttcttgagc agttgggaat tccgtggata ctgtccggcc ttttactgat
     1021 cccctattta tgctgggggg tgtatatcct gagagagcgt ttccatcacc aggcggaact
     1081 cagcgtcgcg tgtgaagcca tcacgttttt aggtctggtg gtttttttcg taatagaatt
     1141 cgcaatgctg agcatgtggc tttcacgctc tccggtattg ttcctgttgg cggtatttgg
     1201 cctgctggca tcccttctgg ccctgtacgg ccccatgttg atgtcgctgg gttcgcacat
     1261 gctggtgggc gcggtaatgc catcgggtgc tccgttaagc cacacgccga aatacggggc
     1321 agccgagggc tttgagcaaa agggggacta cgaggcggct gcgcgtgaat acatggccgt
     1381 cgcgcgaatg tttcccaaag aagcgaaggc ttcacttcgt gccggcgaca atctgatcaa
     1441 actggatcgc gtggaagagg gggttgtgtg gttgaaacgc ggactgcact atctcacgga
     1501 gcccgaggaa tcgctacgtg tggtcaatcg aattgctgaa atttaccatc ggaggctcga
     1561 ccggccggac gaagcgaaag aagcgtttcg cgattatctc agccgtttcc cagacgcgga
     1621 gtatgccgat tccgtaaagc gccgcctcgc gcgcctcggt gggccggaaa cccccaataa
     1681 cgcagaaaaa acctcgccgc gtccaggcga cccgcccata ctggtgtccg aaggcccttc
     1741 gtacctcccc gacgattaag ggtgcctgcg tttacggact cgaggcgcct ggttcgggtt
     1801 gagccacatc ccgatagcga gtgggaatga tcattctggc tccgcccgcg ctgcgcgtga
     1861 tgtcgatcgc gcgcaaaacg cgcccgaaat cggcgccttc gtcggctcgt agaaccagca
     1921 ccgcctcagg ctctgctgtc aagagggctg cgattgattc tttgagccca atctcatcca
     1981 cacgttgctc gccgaaatac agatcgcctt ttgccgtcac cgtgacctca tggatatcca
     2041 ggcgctgttc ttccgcgctc tccgcctggg gcagcgtgat atcaatcccg agatgttcgc
     2101 gaaaggtcga tgagaccatg aaaaaaataa ggagcaaaaa catcacgtca atcagggagg
     2161 tgatgttgat ggttgggcga cgtctggaac gtccgccaaa tgctgaactc atgactgcct
     2221 ttctcaataa cccccatatc cagacggcaa aatggagtcg caccgggaat ggtgcgtgaa
     2281 atcggcccag aacatccagc ctcttttccc ggctggggct gtgtggcccg tagtgcactg
     2341 cccggcatga ttttaggaac gctccgccgg aaatacaagt ccgggatttt tgaaggccgc
     2401 tccttcgctg tgctcgaacc aaagggatgt gacccaggat catctgccgc cggcatttgc
     2461 cggatccgca cctgttcagg tacactctat gttgtgagag gggacgaccc gcagaagttg
     2521 gaaagccgag gaaaaccaag tgcatagctg ttgtatttgc gtggccgaca gcgatgaagc
     2581 cagtgctcga ttgctcgccg atggcctgaa gctgaatgaa tatgaggtgc gtgtagccac
     2641 cacacccgag gacgtctgcc gggaatgcag tcagggggat gttcaggtct tgttgctgga
     2701 tgccgccctg ggggatatgg gcgggcttgc tctagcccgg caattgaaaa cggaccacga
     2761 aacctccggc gtgatcctgc tgctggctct tgatcgggag gcccagaagc gcttttcgat
     2821 cgaagatgcc ctgggcgtgg atgattacat cacaaaaccg tacaacctgc cgatgctcat
     2881 gatccgaatt gagaccctcc tccgcaaacg ccggaaggtg gaatgcctta aaactgaaga
     2941 agaaccactc ggcgacagct cgtacaccga cgagctcaca ggactgcgca accgccgtta
     3001 tctcctggag cggctccagg aagaagtgga aaaagctcac cggtacaatt ttcccgtgtc
     3061 ctgcgtggtg tttgacgtga gcgaagtgga agcgctcgat gatgagctgg gcacggtctc
     3121 tctggatgat ctcctggtgg agctggccat ggcgatgcgc aactacagcc gcacctttga
     3181 catcctcgcg cggtatgacg gtacgatgtt cgcggcggtg ctcccccacg tggatcgcga
     3241 ccaggctgtg caatatgccc agaaaattct aaacgaagtg gaagacacca ccttcgccga
     3301 cccctgctat ccctcgaaaa cccgtttgag cgcgggtatc gtcgcgtgca aaaatggaag
     3361 tgcacgcagc gcagaggcgg ttttgggcga agccatgcag agcctcctgg ccgcccagag
     3421 cgccaaagat aagcgggtcg gtcttcgcga tctttccgac ggcccctgag cgaccgtcac
     3481 cagtctctcc ggcttaattc acccgcccag gcgcttgagc gccttcatcc caatgtctgt
     3541 ccggtaatgg gcgttttcga aatgaatccg gcggacggcc gcataggcgc gctcgattgc
     3601 cgccggcaca tccgaacccg tcgcggtcac gttaagcact ctgccgccgt tggtaaccag
     3661 ggtgctccct tccatcgcgg tgcccgcatg gaacaccacc gcaccagcct cttgttctgc
     3721 ctccgggatg ccggaaatcg ctttgccctt ctgatacgcg cccgggtatc cgccggacgc
     3781 catcacaacc gtgacacacg cgccttcttc ccactccagg gacacatgtt ccagcgtacc
     3841 ctcacagcag gcgaccaaga tcgggatcag gtcgttcttc attcgaggca acacgacctg
     3901 cacttccgga tcgccaaaac ggcaattgaa ttcgatcact ttggggccat ccggcgtaat
     3961 catgaggccg gcataaagga cgccggtgta gggtgtgccc gcttccgcca tcgcctgaac
     4021 gcatgggttc aaaatcgtgt tcattatctc gtgctgcaac gcatcggtca cgacgggtgc
     4081 cggcgagtag gcccccatcc cgccagtgtt cggcccttcg tcgttgtcaa acgccggctt
     4141 gtgatcctgg ctcgacgcca tgggaataac cgtcttccca tcggagaagg cgagtattga
     4201 cgcctcttcc ccttcgagat attcctcgac cagcacgcga tttccggcgt cgccaaaggc
     4261 ccggtcttcc atggcggccc ggatcgctga gaccgcagtg tccagatccc gggcaaccgt
     4321 cactcctttg cccgcggcca gcccatcagc cttgatcacc accggtgcgc cggtgctacg
     4381 cacatagccg atggcctcgt ccgggcacat gaattccgcg gagcgggcgg tgggaatccc
     4441 acagcggttc atgacattct tggcgaagct cttgctggct tcgaggcggg cggcggcggc
     4501 aaccggacca aatgtcttga taccattcgc ggtgaggtaa tccaccaacc cccgtgacag
     4561 tggatcttcc ggtccgacaa cagccagccc cacgccggca tcccgggcca gggcgcccag
     4621 cttttcgaaa tcatttacgc cgatatcgac acagcgcccg agcgcagcca ttccgggatt
     4681 gccgggcgcg cagaagaggc gctctacctg cgggctttgc gcaattttcc acgccagggc
     4741 gtgctcacgt cccccgcttc cgacgatcaa tacattcacc tgtgcaacct cccagcgtca
     4801 gcgcgtatca cccgccattc caggccaatt gtgtgttctt gaggaattcc gcaatggcgt
     4861 gatcgtagcg cgcggcactc gcaaacgctt cctgtgcgag acggtggcgt atttcaaacg
     4921 tcagcgctcc accgtgcgcg cgcatttcgt gcatgaccag gctgtagcgc tccggattta
     4981 ccacagcacc cacgtagcgg aaattctttg cggccgaacg gatcatcgac aggccgccga
     5041 tatcgatctg ctcaatcacc tcttccggca tcgctccggg ctgtgcgatc agggcctcaa
     5101 ccgggtgaag gttggctacc acgaggtcga tccattcata accgtacgcc tggagctgct
     5161 cggcatgcag tttgttatcg cggatgccca gcagaccggc gtgaatcttc gcatgtagag
     5221 acttgacccg cccgtccatc atttcttcca caccggtgta atcggccatg ttcaaggcag
     5281 ggatcccggc ttcctcgagg acacgcagag tgcccgccgt gctgatcagc tccacgccga
     5341 attcggccaa caattgagcc agctcaacca gcccggtttt gtcgtggcaa ctgatgatgg
     5401 ctcgtctgat cggtgtcatc gctctggctc cttcccctgg ctccagcggt ctgcaggccg
     5461 cgcgcccgta tttgaacggg cgcatatagt acttcaggtt cgccggaaat gaaaaacctg
     5521 tgcccggcca acacaaactt gattcgctcc gggccgcttc acaggggcgg ctggatggct
     5581 ccatagtggc atcgcccttc attcaacatc aggagcctgt cgacaagcgg ccatgcttca
     5641 cggcacccct gtgtggcaat gatgactgtg agatgctcag ctgagcggcg cctcagaatc
     5701 tcttccgtaa actgctgcac agcggcacgc tcgagatagg tgaacggttc gtcgagaagc
     5761 aggaggcgcg gattcacgat cagggcgcgc gcaagcgcca gccgctgctg atgcggcaga
     5821 gaaagcgacc ccggccgctc gcggcgcttg ccggccagtc cgctcaaggc gagcatgtgc
     5881 tccacccgtt cgatccgctt ttcccgcgag atctcctgcg catgcaggac aaagaggagg
     5941 tgcttctcga ccgacatatg cggccagaga gcaagttcct gaaataccat gccgattgca
     6001 cgcgtgtgaa gctcgcccgc tgccgcaggg acatcgtcca cggtgacgct gccggcgctg
     6061 gggagttcga gtccggctac cagccgcaac agcgtgctct tgcccgaagc ggccgggccg
     6121 gacaggcaca gcccttcgtt tggagccagt gtagcggaga tgccctgaaa caccggcgtg
     6181 ccgcgataga cctttgtgac gctgtcgaat ttaacggtag gcatggagca cccgcccgca
     6241 cgcgaagcag aacaccgccg tgcacaagcc cgttgcgaac agcaggcttg ccattgcgag
     6301 gcacagcgac gccacggcag cactgggccc caaatgcatc agagaatgaa cacggaccgg
     6361 gagtggcatc acgtcgggcg aagcgaccat taccaccgcc ccgagttcct gaagggaaag
     6421 gaggcatgcg atgccccaga cggccacgtg aactggcgct gccctgtgaa ttcgtgccgc
     6481 cgggagacgt ggacgggaca gtgccagtaa ggcgatgcgt tcactcaggt atgcgaaata
     6541 aaggtatttg gcggtgttgg ccaggaccag gattaaaaac gatcgggata caaacccggt
     6601 ggggccggga tgattccaaa gggctctcaa acctgtgccc agcaacggcc ccgtgagcag
     6661 aaacgggatg aggctcaatc ggaacagcag ccccaaacgt ggactgtcat tggcaaacca
     6721 ggccatggcg aaccctaatg cggtaagcac ggttgctgcg gtggcggatt gcagaagact
     6781 cgtcaggatc tcacgccgtg catccgacca cgcgagcatc agctgcgccc agctctcgat
     6841 ccgccccgcc aggagggaca gaggcacgcc gactgccaga gccgccagaa acaaacaggc
     6901 gccagacgcg agcagtgccc gtttccagcc caccggcacc cggccggccg gtttgggtgt
     6961 ggatgccagc cactgctggc gagggcggac ccagcggccc caggcccaaa ggatggcact
     7021 tcctgccgcg agcaatggca atgcctgcgc gaccgcccgg cctgtttcat gaaaggcgct
     7081 caattcagta aaaatctcca ccgaacaggt attcacctga aacaaagccg gaacggcagg
     7141 ttccgtgagc gttaacaaaa acacaagtcc cacccctgtc aagacccacg gcgcgcacaa
     7201 tggaaaacgc accacgaaaa aagtctgaac cggatcggca atcagccggg ccgattccag
     7261 catccgccgg tcgaagcgct gcaatgccgc aacggtacag aggaacacga acgggtagta
     7321 cgacaacgta aaaaccagga tgacgctcag gaggcggaat ggaaatggca cgcccggttc
     7381 tgctgtaaac tgcggcgcca ggatccccat gcgcgtcagg gcatctagcc aggccgccga
     7441 aaagacgcag ggcgggatca gcaccggcac gcacagtaga aacgtgaaaa atgcccgcaa
     7501 aatacgcgga caatactgca gggaaagcgc aatgcctccc cccgccaaca gtgacgtgag
     7561 gcacgtaccc aggctcaccg ccagggtgtt ggcgagtatg gtcgcctgcc tgggttctgc
     7621 gaagacagca cgccaggttt cgaagcgcca gacactgcct tcaaacagag aacgccaacc
     7681 gagcgcggcc agaggcgcca cactgacagc ggcaaaaacc gccaaggtca atcccctgaa
     7741 aaaattatga ggcatacggg gcttgggcat ttcttcagaa gtcccctgag aataaacgca
     7801 gccttcgatt ccagccaccc ggtcgcagcg ctgaactgga cactcattgt atgaattcca
     7861 ggcagccggt gcacattctt cagatgcgtc acctcgagat tcaagcggtg ccgtggattg
     7921 tccgggaagt cgtggcctgc tcggacacgc cggccgatcc atgcggtggt ctacagggag
     7981 cggccttgtt ttcgccagat cgcgagctgg cggctgcggc gttccgcctg gccacgccga
     8041 acctgtttca cccaggttgc gtagtcttct tcgtcaggaa gcatcaacgc tatgaaggaa
     8101 tccaagaggt catcccccag aaagtcgcag aatgaatcat agagctggac atggttcgcg
     8161 tacagcatgc cgcgagagta tcgcttcact gggacaatgc tgtccaggtc gataaaatag
     8221 aacttttcgc cgtttcgcgt gaagatgttc tttcctgaca aatcccggtg atacgcgccc
     8281 gcgtcccgga gtgcatttac ggtttgcgcc aggtcatgca gaaaagtgcg gagccgttcc
     8341 tccggcgcgc cgtcctgaac catttccgcc atgcaccgct ccacattgtt cgcaccctcg
     8401 agatactcca tgatgaggta actggcgcgg atgatgcccc agcgtccttg ttccaccagc
     8461 ccgtccacac gcggcgcccc aatgcccagc tgttccaggt acagcccggc cacccagccc
     8521 cgccggtagc gctcacggcg cagagtgcgt ttcagcactc ccagcaccgg ctcgcgcgaa
     8581 caggccttca ccacatactg accgacccgg cgcgttatgg tcgttgggga gatctgcaga
     8641 gtatccccct cggctgcgat caattccag
//
LOCUS       DAOPBQ010000073         8659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7611_length_8659_cov_4.599024, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000073 DAOPBQ010000000
VERSION     DAOPBQ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7611_length_8659_cov_4.599024"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2418)
                     /locus_tag="PKW60_03770"
     CDS             complement(<1..2418)
                     /locus_tag="PKW60_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPJ98579.1"
                     /translation="MRIRRLFLDGYGRFTGREIEFAGGLQVVLGANEQGKTTMRSFIT
                     DMLYGQKAGSTLRRYDENHQLRRPWADTRAYGGRMTYLLDNGQEFEIHRVFERGNESV
                     HVLNLSDGRDATGDLVRTRNQELEFAARQLGLSKAIFANVAVIGHHSLESLGDEDALH
                     QIGEKIAALADTADEFSSAATAVGHLDAYTARIGRLVPHSKRPLPLARARLGQVETEL
                     TEALRVRDEVAGLEKRLRAARAEFDALRRSQMELELQLRRLEHTERITRLQKAENIQY
                     QIETLTQRLFSLAKAKDFPLEQEDVFNRALNAMETAREQVARTEARMTELLRRHREVS
                     HNLENDAVEIQDLPEQFEDRLKELDTQIARLRDFIEDFEKAQQTVRDAIARAGRELED
                     LPDFAAVSAEPLAWINQQATSFSLAVQERDHAVEAFERLEAECARREAELAQPEKVFG
                     QFEDFPAQARDHAVEAKLSEERCAQQRKRIEEAQRDLEVHEEDLPNFRAMTLFGALLS
                     IILLISAALTGNKGVYFAEVFALGVLVYNLAHWVHTRLTLRQLHHAAEAAGEEIARLE
                     SATAEREQAIDELIREAGCESFRELEALYDQYVKGRNELEARREELEQHRHRARVEES
                     HVAGKLERLQAAFADLHETVETEDAVEAAAACAIAKYQEYRELSRRREEQMEQLETLT
                     LECDRASEKLAVKEGEEKRLSLEVRKLLREAGYREEANHTSALGALRAYGIRIAQLRP
                     RRARADALKQQADEFAQQLEQDRRELEQAQEDLSQLLDNVGVDAVDEYRALAAQAREY
                     REARILRT"
     gene            complement(2421..3569)
                     /locus_tag="PKW60_03775"
     CDS             complement(2421..3569)
                     /locus_tag="PKW60_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ98580.1"
                     /translation="MRLIHTADVHLDRCFASAHTPPGFGNRRRQSLRDVFHAIVRRAG
                     EWPADALLIAGDLFDLERVTRDTVRFLQAEFESIRHVPVCIAPGNTDPFVPGSPYAEE
                     EWPGNVMIFDQPQWRSFVLDGNGTTVHGFGFDGPDVPASPFGTFPPIQLGATHVAVAH
                     ASALGHQPPGKAACAPFEPATAAVDGLRYLALGHFHAYTPISGPCPTVMAYSGAPEGL
                     GFDETGMRHYLEVKIDGDRVHVTPVASARSVYLAESIDCSALSCPQELIESIRALPRE
                     EGVGVIAHITLTGSISAELRAEMDAIHDAVASRFEQVFLNDRTELSEDMAALARHQTS
                     LGGFVARLNAALADAASDRERRLLARARELGLAAYRNRPIAFQGLERD"
     gene            complement(3762..4712)
                     /locus_tag="PKW60_03780"
     CDS             complement(3762..4712)
                     /locus_tag="PKW60_03780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPJ98581.1"
                     /translation="METTDLTVVTLTRGQPQVLSMCLNHLELQTYPAARFEVIVVAFP
                     GATGTTAMLERFCSGGPVRMRCVHAPEENVAAARNLATMQAQGQWLLFLDETLLAGSH
                     LVENHVKCQQRNGNAAAAVGRISYHPQFGARTASKAWRMTQQAFQPGQPLSFLDWRAR
                     NLSLPRDAVLEAGGFNEDLPFEGLEAMELGYRLERSTGIRGCYCDQAQAFVWKPESLG
                     REVERSYAEGYWLHHLFETTESEQLRERYRGMVSAWRGAADPIMLPLAHRLCPALAYN
                     SRTFNALRFRALRSAFRRGYRDANRGRPAVLPVRYMPDES"
     gene            5229..7595
                     /locus_tag="PKW60_03785"
     CDS             5229..7595
                     /locus_tag="PKW60_03785"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPJ98582.1"
                     /translation="METTIPQSSRYGSIALFLLAVTFVIAAPFYWQQVELTDVPASES
                     YENSDLYQFVYPAMHYAYGRMRSGHLPQWNSRQLCGVPLLADHRLGLFQPLNAVFLLS
                     DTGHAMALHSFICLALMGFGCALFARSLGLNYLAALVGGAVYAFAGASAAAMSRPHLA
                     SALAWMPFLFWAGREFAHSGRRSWALLAGLAGALFILSGAYAIVAVTLPVLVLYVILH
                     GFTRRPGEFGLRIALSGIVLGGTLAVCLSAIQWIPTAVWAMALDSPSAALWYLRSGGL
                     LPGSFREAVVQTFSSRPGGLPRLCYVGMVPLILVPAACFQRKNWREVLLFLFVGAGAW
                     TLGIAGPQRVAGGLPRETFLYLVVFCVAVLTALGADRIFKPRKDYRSVQVWPVAIVVL
                     LTLCGVFYVAGSQGRGYVIAFAILLTPFLTLRQTWVSILVSCAVALLIFIDLTLANLN
                     AYGHPYQDYPEHARRYSDSVEIARERALGGRVAVLSRPLDYGLPANLGMLAPIDVAGG
                     CNLFVTREQRSWWSRLIRQGADAEPAVSWDVSPDAAQPSLLNFMAVRAILASPDTRIP
                     VEAWNAAGLRLREIQTPARGDIVLNESALPRAFWVPAWRFADGVEAAMAALTAPGFDP
                     SQECVVDAAYGPDLERFQRRGPGQPETPRGEVVAAALRSTPPAVIPAPAALPAGQLPA
                     TPRLMPSIGTLEEQSPERLTVHIEVPQSGIVVLCDSFARGWRASLNGEPCEILRVNGV
                     FRGVAVPAGVHDITFTYRPASLYLGTALTLFGAALLLGSLFTFSRQRP"
     gene            complement(7848..>8659)
                     /locus_tag="PKW60_03790"
     CDS             complement(7848..>8659)
                     /locus_tag="PKW60_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98583.1"
                     /translation="FRVACELVRNGVAGEISRVECSFGPPGVPCDLPEEEMEPGLDWD
                     LWLGPAPVRPYNSILSPRGVHDHFPDWRNYREYGGGMVTDWGAHHLDIAQWGLDMDES
                     GPVEVRPSEDPAAVDGGVLQYANGVQVIHMKGFGAHFYGSDGEVKVNRGKIELVLHGE
                     KVAGFVDRDSGGSLESTLQQLENEYLSDAKVRLYQSYDHIQDFLDCVKSRRKPITSEV
                     VGGRSAICCHLLNQAYYNHATIKWQPERMQFAPGGGDPAWLTRDYRAPWSV"
BASE COUNT         1428 a         2807 c         2657 g         1767 t
ORIGIN      
        1 ggtgcgcaag atgcgggctt cacgatactc ccgtgcctgc gcggccagcg cccggtattc
       61 gtccaccgca tctacgccaa cgttgtccag caattgcgac agatcttcct gagcctgttc
      121 gagttcgcgg cggtcctgtt caagctgctg cgcgaactcg tcggcctgct gtttcagagc
      181 gtcagccctg gcccgcctgg ggcgcaactg ggcaatgcgg atgccgtagg cgcgcaacgc
      241 gcccagtgcg ctggtgtggt tggcttcttc tctgtacccc gcctcccgca gcagcttgcg
      301 cacctcgagc gacaggcgtt tttcctctcc ttccttaacg gcgagttttt cactggcgcg
      361 gtcgcactcg agcgtgagcg tctccaattg ctccatttgt tcttcacgcc ggcggctgag
      421 ctcgcggtac tcctggtatt tggcaattgc gcatgccgcc gcagcctcta cggcgtcctc
      481 cgtctcaacg gtctcgtgaa gatccgcgaa cgcggcctgc aggcgttcca gcttgccggc
      541 tacatgggat tcttccacgc gcgcccggtg gcgatgctgt tcgagttcct cacgccgtgc
      601 ttccagctcg ttgcgcccct tgacgtactg gtcgtagagc gcttcgagct cccggaagga
      661 ctcgcatccc gcttcgcgga tgagttcgtc gatggcctgc tcgcgctcgg ccgtggcgct
      721 ttcgagccgg gcgatttcct cgccggcggc ctcggcggcg tgatgcagct ggcgcagggt
      781 caggcgcgta tgcacccaat gggccaggtt atacaccagc acgcccagcg cgaagacttc
      841 agcgaaatac acgcccttgt ttccggtgag cgcggcactg atcaacaaaa taatcgacaa
      901 cagcgcgccg aacagggtca tggcgcggaa attgggcagg tcctcttcat ggacttcaag
      961 gtcgcgctgg gcctcctcga tgcgcttgcg ctgttgtgcg cagcgttcct cggacagttt
     1021 ggcttcgacc gcatgatccc gcgcctgcgc ggggaaatcc tcgaactgtc caaagacttt
     1081 ttcgggctgg gccagctcgg cttccctgcg ggcacactca gcttccagcc gttcgaaggc
     1141 ttccaccgcg tggtcccgct cctgcacagc caggctaaag gaggttgcct gctggttgat
     1201 ccacgccagg ggctcggcgc tcaccgcggc gaaatccggg aggtcttcaa gttcccgccc
     1261 ggcccgggcg atcgcgtcgc gaacggtttg ctgcgccttt tcaaaatcct caataaaatc
     1321 gcgcagacgc gcgatctgcg tgtcgagttc cttgagccga tcttcaaact gctccggcag
     1381 atcctggatc tccacggcat cgttctcgag gttgtgtgaa acctcccggt gccggcgcag
     1441 caattccgtc atgcgtgcct cagttcgcgc cacctgttcg cgagcagttt ccatggcgtt
     1501 caacgcgcgg ttgaatacgt cttcctgttc gagcggaaag tctttggctt tggcgagcga
     1561 aaacagccgt tgcgtcaggg tttcaatttg gtactggatg ttctcggcct tctgcagccg
     1621 cgtgatgcgc tccgtatgtt ccagcctgcg gagctgcagc tccagttcca tctggctgcg
     1681 gcggagggcg tcaaattcgg cgcgggcggc gcgcaggcgt ttttccagtc cggccacttc
     1741 atcgcggacg cggagcgcct cggtcagttc ggtttcgacc tggccgaggc gcgccctggc
     1801 cagcggcagc ggccgctttg aatgcggcac caggcgtccg atgcgggcgg tatacgcatc
     1861 gagatggccg accgccgtcg cggctgaact gaattcgtcg gctgtgtcgg ccagtgccgc
     1921 gattttctct ccgatctggt gcagggcatc ttcatcgcca agcgactcca ggctgtggtg
     1981 gccgatcacc gcgacgttgg cgaagatggc cttgctcagc ccgagttggc gcgcggcgaa
     2041 ttccagttcc tggtttcggg tccggactaa atcgccggtg gcatcccggc cgtcgctgag
     2101 gttcagcacg tggacggatt catttccgcg ctcaaacacg cggtgtatct cgaattcctg
     2161 cccattatcg agcagatacg tcatgcgccc gccgtaggcc ctggtgtccg cccaggggcg
     2221 ccgcagctgg tggttctcgt cataccggcg cagcgtgctg ccggccttct ggccatagag
     2281 catgtcggtg ataaaactgc gcatggtggt cttgccctgc tcgttggcgc ccagcaccac
     2341 ctgcaatccg ccggcaaact cgatttcccg gcccgtgaac cgcccgtatc cgtccagaaa
     2401 cagcctccgg attctcaccg ctagtccctt tccagaccct ggaacgcaat gggccggttc
     2461 cggtaggcag ccaggccgag ctcccgcgcc cgagccagca gtctgcgttc ccggtcgctg
     2521 gctgcgtcag ccagcgccgc attgagccgc gcaacgaatc cccccagact ggtctggtgg
     2581 cgtgccagcg cggccatgtc ttcggacagc tccgtgcggt cgttgaggaa cacctgttca
     2641 aaccgggagg ccaccgcatc gtgaatggca tccatttccg cccgcaattc ggctgagatc
     2701 gagcccgtaa gcgttatgtg cgcgatgacg cccacgccct cctcgcgcgg aagcgcacga
     2761 atggattcga tcagctcctg cgggcacgac aacgcgctgc aatcgattga ttcggccagg
     2821 tacaccgaac gtgcggaggc caccggcgtg acgtggacgc gatctccgtc gatcttcact
     2881 tcgaggtaat ggcgcatgcc ggtctcgtca aaaccgagtc cttccggcgc gcccgaatac
     2941 gccattacgg ttggacatgg cccggaaatg ggcgtatagg catgaaaatg acccagcgca
     3001 agatagcgca ggccgtctac cgcggccgtg gccggctcga agggcgcaca tgcggctttc
     3061 ccgggcggct ggtggcccag cgctgaggcg tgcgccaccg cgacatgcgt cgcgccaagt
     3121 tgaatcggtg gaaacgtgcc gaaagggctg gccggcacgt ccgggccgtc aaacccaaac
     3181 ccgtgcaccg tcgttccgtt cccgtcaagc acgaaagagc gccattgggg ctggtcgaaa
     3241 atcatgacgt tgccgggcca ctcttcttcg gcatacggtg agcccggcac aaaaggatcg
     3301 gtattgcccg gcgcgataca tacgggcaca tgccggatcg attcgaattc tgcctgcaga
     3361 aaccgcaccg tgtcgcgggt tacgcgctcc aggtcgaaga ggtcgcccgc aatcaacagc
     3421 gcatcggcgg gccactcgcc cgcacgccga acaatggcgt gaaacacatc gcgcaggctc
     3481 tggcgcctgc ggttcccaaa ccccgggggc gtgtgcgccg aagcaaagca cctgtccaga
     3541 tgaacgtccg ccgtatgaat caaacgcata gtcgtctacc cggtgaatcg tgttcagagg
     3601 ctcgataccc gccggagccg gcacgcccct ttcgtgcccg ccgctgcgcc ccctgggcgc
     3661 ccgcttcacg gtgcagcaca agcccgtctc catcataata cgttttcggg ggcggttgaa
     3721 agagtgcgaa cggccggccg gagaaattcg cccagctcga gctaggattc atccggcatg
     3781 taccggacgg gcaggacggc gggccggccg cggttcgcgt cgcgataccc ccgccggaac
     3841 gcactgcgaa gcgcccgaaa gcgcaaagcg ttaaacgttc gagagttgta cgccagcgcg
     3901 ggacacagcc ggtgggccag agggagcatt atcgggtctg ccgcaccgcg ccaggccgat
     3961 accatgcccc ggtaccgctc gcgcaattgc tccgattccg tggtttcgaa gagatgatgc
     4021 agccaatatc cctccgcata ggagcgctcg acttcccggc ccagagattc cggcttccag
     4081 acgaatgcct gagcctggtc acagtaacag cctcgaattc cggtgctgcg ttccaggcga
     4141 taacccagtt ccatggcctc caaaccctcg aaaggcaggt cttcgttgaa tccccctgct
     4201 tccagcacgg cgtcccgcgg cagactcaaa ttccgggcgc gccaatccag aaaactcagc
     4261 ggctggccag gttgaaatgc ctgctgtgtc attcgccagg ccttcgacgc agtgcgtgcg
     4321 ccgaactgcg ggtggtagct gatccggccc accgccgcgg cggcattgcc gttgcgctgc
     4381 tggcatttca cgtgattttc cacaaggtgg cttcccgcaa gcagcgtttc gtcgaggaac
     4441 agcagccact gcccctgggc ctgcatggtg gccaggttgc gtgccgcggc cacattttcc
     4501 tccggcgcat gaacacagcg cattcgcacc ggcccgcccg aacagaaccg ttcaagcatg
     4561 gctgtggtac ccgtggctcc ggggaaggcg actacaatga cctcaaacct ggccgccgga
     4621 taggtctgca gctccaggtg attgaggcac atggataaca cctggggctg gcctcgcgtc
     4681 aatgtgacga cagtcaggtc agtggtttcc ataccgtact caccctcgca gatgttcaga
     4741 gcactcctac ctcatcctac ggataaagac agcgcagatt caaggaaacc acggccacag
     4801 cccggaggat tcacaccctg aggtgcagcc gcctgaacga caagctgtcg cacccattga
     4861 tttctcacca ggcatggagg ttgatcgtgg gattcaggtt ctgtcatctc tccggaaggg
     4921 aagaagtctt ccgtttgagc tgtgggctgg gttctatacg ccggcgagga ggcagcttga
     4981 ggcggcctca gggcatctga catcgccgtg tcttccattt tccggtaaga tcgcaaatgg
     5041 caagacgata ccatccccgg taagatctta gatggcgcga ttcggtcaat ggacactctg
     5101 gcgcgtgcaa tgatagactc tcgtttggcc cgtgcggcgg ctgggccctc ctgcccgttc
     5161 tgtgaagaac acggcgcgtt ataaccggat gaactgttga aatggcagcc tgagaaaggg
     5221 ccaggcgcgt ggagactacg atacctcaaa gctcccgcta cggaagcatc gcgctgtttc
     5281 tgctggccgt cacattcgtt atcgccgcgc cgttttactg gcagcaggtg gagctgacgg
     5341 atgtgcccgc cagcgaatca tacgaaaaca gcgacttgta ccagttcgtc taccccgcga
     5401 tgcattacgc ctacggccgc atgcgaagcg gacacctgcc tcagtggaat tcgcgtcagc
     5461 tgtgcggcgt accattactt gccgaccatc ggctcggcct ctttcagccg ctcaacgccg
     5521 tgttcctgct ttcggacacc ggccacgcca tggcgctcca ttcgttcatc tgcctggcgc
     5581 tgatgggctt cggctgcgcc ctgtttgcgc gctcgctcgg cctgaattat ctggcggcac
     5641 tcgtgggcgg tgctgtctat gcgttcgcgg gcgcatccgc cgcggccatg tcgcggccac
     5701 atctcgcctc cgcgctcgcc tggatgccgt ttctgttctg ggcaggccgc gaattcgcgc
     5761 attccggccg gcgcagctgg gctttgctgg ccgggctggc gggcgcgctg ttcattctgt
     5821 ccggcgccta cgcgattgtg gcggtgaccc tccccgtgct ggttctgtat gtcattttgc
     5881 acggatttac acggcgcccg ggcgagttcg gattgcgcat tgccttgagc ggcatcgtcc
     5941 tgggtggaac gcttgccgtc tgcctgtcgg ccattcagtg gattcccaca gctgtctggg
     6001 cgatggccct cgattcgccg tcggcggcgc tgtggtacct ccggtcgggg ggattgctcc
     6061 ccggctcgtt ccgggaagcc gtagttcaga cgttcagctc gcggcccggc ggtttgcccc
     6121 ggctgtgtta tgtgggcatg gtgccgttga tcctggtccc ggccgcctgt tttcaacgca
     6181 agaactggcg ggaagtgctg ctgtttctgt ttgtgggcgc gggtgcctgg acgctgggca
     6241 tcgcgggccc gcaacgggtc gccggaggcc ttccccggga gacctttctg tacctcgtcg
     6301 tattctgcgt cgccgtcctt accgctctgg gcgccgaccg catcttcaag ccgaggaaag
     6361 actaccgttc cgtgcaggta tggcccgtgg ccattgtggt gctcctgacc ctctgtggcg
     6421 tattttacgt ggccggaagc caggggcggg gttacgtcat cgcgttcgcc atcctcctga
     6481 cgccattcct gaccctgcga cagacctggg tatcgatcct ggtgtcctgc gccgttgcac
     6541 tgctcatttt catcgatctc acactcgcca acctgaatgc gtacggtcat ccgtaccagg
     6601 attatcccga gcacgcgcgc agatacagtg attccgtcga gattgcccgg gaacgggctc
     6661 tgggcgggcg ggtcgcggtg ctgtcacggc ctcttgacta cggcctgccc gcgaatctcg
     6721 gcatgctcgc gccgatcgac gtcgcaggcg gctgcaacct ctttgttacg cgggaacagc
     6781 gcagctggtg gtcccggctc atccggcagg gcgccgatgc cgagccggcg gtatcctggg
     6841 acgtatctcc tgacgctgcc caaccctcgc tgctcaattt catggcagtc cgtgcgatcc
     6901 tcgcgtcgcc cgatacgcgc attccggttg aggcctggaa cgcagcgggc ttgcgcctgc
     6961 gggaaatcca gacgcccgcc cgcggggaca tcgtcctcaa cgaatccgcc ctgccccgcg
     7021 cgttctgggt gccggcctgg cgctttgctg atggcgtgga ggccgccatg gccgcgttga
     7081 ctgcgcccgg ctttgatccg tcgcaggaat gtgtggttga tgcggcgtac ggcccggatc
     7141 tggagcggtt tcagcggcgg ggacccgggc aacccgagac cccgcgcggt gaggtggttg
     7201 cggcagcctt gagatcgacg ccacccgccg tgatcccggc acctgcggct ttgcctgcag
     7261 gccagttgcc ggcgacgccg cgcctcatgc cgtccatagg gacgctggaa gagcaatccc
     7321 ctgaacggct caccgtccat attgaagtgc ctcagtccgg catcgtcgtg ctctgtgatt
     7381 cgttcgcacg gggctggcgc gcatcactga atggcgaacc gtgtgaaatc ctgcgcgtca
     7441 atggcgtatt tcgcggcgtc gccgttcccg cgggcgtgca cgatatcacg ttcacctacc
     7501 gcccggcctc gctgtatctc gggacggctc tcaccttgtt cggcgcagcc ctgctgctcg
     7561 gctccctgtt tactttctcc cgccagcgtc cctgatgccg gatgggctcg cccttcatct
     7621 ctccagcagt atcgtaacga aaccggcatg agacgcccca ccggtgagtc tcgtggcatt
     7681 ccgtacgaat cgttcggagt caggaaaaat cgagagcact ttgcagctgt ccgaaaagcg
     7741 cgtggccatg atttgaaggc ccgccctggc gcgatacaaa aacacggcgc caacaggatg
     7801 tcagcgctgt gcctgtataa atcgacaaga cgatcggctg ttttcgtcta gacgctccac
     7861 ggtgcgcggt agtcgcgcgt gagccacgca ggatcgccgc cgccgggcgc gaactgcatg
     7921 cgctcgggct gccatttgat cgttgcgtga ttgtagtacg cctgattcag gagatggcag
     7981 cagatcgcgc tgcggccgcc caccacttcg ctggtgatgg gtttgcggcg cgatttgacg
     8041 cagtccagaa agtcctggat gtggtcatag ctctggtaca gccgcacttt cgcatcactc
     8101 agatactcgt tttcgagctg ctgaagggtt gattcgagag agccgccgct gtcgcggtcg
     8161 acaaatcccg cgaccttttc accatggagc accagctcga tcttgccgcg attgaccttg
     8221 acctcgccgt cgctgccgta gaaatgcgcg ccaaaacctt tcatgtggat gacctgcacg
     8281 ccgtttgcgt attgcagcac gccgccatcc acagcggccg ggtcttcgga tgggcggact
     8341 tcaaccgggc cgctttcatc catatcgaga ccccattgag caatgtcgag atgatgcgcg
     8401 ccccagtcgg tgaccatgcc gccgccgtat tcgcggtaat tccgccagtc ggggaagtgg
     8461 tcgtgcacgc cgcgcgggct caggatcgag ttgtagggcc gcaccggcgc ggggcccagc
     8521 cacagatccc agtccagccc gggttccatc tcttcttccg ggaggtcgca ggggactccg
     8581 ggcgggccga aactgcattc tacgcggctg atctcgccag cgacgccgtt gcgcacgagt
     8641 tcgcaggcca cgcgaaact
//
LOCUS       DAOPBQ010000074         8654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7616_length_8654_cov_4.234562, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000074 DAOPBQ010000000
VERSION     DAOPBQ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7616_length_8654_cov_4.234562"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PKW60_03795"
     CDS             <1..188
                     /locus_tag="PKW60_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98584.1"
                     /translation="GDELDEIIRKHGGKDLLPEKETPPSGPQPSRSAVPEQPHDEEEP
                     DSGGLQPGPEAPVPHPA"
     gene            242..1087
                     /gene="folP"
                     /locus_tag="PKW60_03800"
     CDS             242..1087
                     /gene="folP"
                     /locus_tag="PKW60_03800"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPJ98585.1"
                     /translation="MTKGATEYLLAERLGSGTLVMGIVNVTPDSFFDRGRHATCECAV
                     AHVHEQAEAGAGIIDIGGESTRPGAEPLTPAAELQRVLPVVRAAAPLQVPLSIDTYHA
                     STAAACIAAGAAMVNDITALRGDPEMAGVIASSGVDCVLMHMQGEPRTMQANPSYTDV
                     VDDIRAFFDERMAYAVAQGIPEQRIWLDPGFGFGKPVAHNLELLRRLKELNALGRPLL
                     VGTSNKSMIGAVLNAPMEERDAGTAATVAVAIMNGARAVRVHNVRMMVRVARMTDAIC
                     GKYDP"
     gene            1084..2427
                     /gene="glmM"
                     /locus_tag="PKW60_03805"
     CDS             1084..2427
                     /gene="glmM"
                     /locus_tag="PKW60_03805"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPJ98586.1"
                     /translation="MSERLFGTDGIRGIANRYPMSAELALQVGRAVGHIFQQEERQHT
                     ILIGKDTRRSGYMLENALTAGLCSMGVHVLLVGPLPTPGVSYILRSLRCDAGIMISAS
                     HNAFQYNGIKLFGPDGYKLPDSVEDDITRLIQNGEVEDIRPVAERVGTARRIDDAVGR
                     YIEFAKSCFPKGMRLDGLKIVCDCAHGASYKVGPSTFSELGAEVIALGNHPNGTNING
                     GCGSTHPEDMRATVIRERANVGIAFDGDADRIVMADEEGRLIDGNGLLAILACDMLET
                     KRLPKNCLATTIMANGGLLKAIEPLGGQVVWTKVGDRYVVEAMREHELALGGESSGHV
                     VMLEYNTTGDALIAALQVLATMVRRDAPLSVLASAYQQMPEAHAHVPHDNGAGPSEEK
                     LAEIVREAEAKLKGRGRVVIRASGTEPIIRVMVQHELRREAESLARELAKQVASL"
     gene            2443..3171
                     /locus_tag="PKW60_03810"
     CDS             2443..3171
                     /locus_tag="PKW60_03810"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPJ98587.1"
                     /translation="MIELGVNIDHVATLREARKGAAPDVITAAKLCELAGAHQITVHL
                     RQDRRHIQDRDVELLKQVLQVRLNLEMAAADEIIEIAQRIRPEFVCLVPERREEVTTE
                     GGLDVAGQIKRLTEVTAGFHGVGVRVSMFIDPEYEQVDASAEIGADCVELHTGAYASA
                     RGEEALVELDRIVEATGRALDAGLGIHAGHGLSIRNLHAVAAIPGISEVNIGHSIVAR
                     AMFIGLEEAVREILDILDAYPEED"
     gene            complement(3276..4073)
                     /locus_tag="PKW60_03815"
     CDS             complement(3276..4073)
                     /locus_tag="PKW60_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98588.1"
                     /translation="MDTRIRAGETPLAARGPLGHTTRGQERPVGGDRLLRPEEAAIRL
                     SILERRRTEVLQTARETTSLQHGIHAVRMTLGGLQKQQESLARMRELAGQASDGERTE
                     VQRYALHETTRQLLGQFDETAGQTAVHWRHLVSSDSGGISWQDVNGELPALPESSAAA
                     LGIHDLDLSTPEHAAESVQTLDKAAAQLSEIQADLRAKEEEFVAAIEQRQTAAQPAAL
                     AGSRIRDRAAAQQALEQMRNRLLQPGNTAAQMQPNLRGETAARLLNS"
     gene            complement(4204..5514)
                     /locus_tag="PKW60_03820"
     CDS             complement(4204..5514)
                     /locus_tag="PKW60_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98589.1"
                     /translation="MKAAAAAGTGLLILPSGTRIGAAAPSNKLNVALIGAHGRAMAHY
                     DPIAEENVVALCDVNEDHIALAAERFPKAKRYIDWRKCLEQKNLDAIICCTTDHTHAF
                     VSTWAMNRGLHVYCEKPLANSVAEARLVRETYLKNKDKVATQCGMQRHAHENFQRVSE
                     LIRDGIIGELEEVHAWGNRQIPKPGYLPAEGKPPKTLHYDLWLGPSPFHPYNPGYFQG
                     APGANCLCWNMYWDFGSGQTGDMGSHTMDIVWCAIDAEQPTSAEATGDPFNPEVTPVE
                     LTATFEHPANAWRPPIRVTWHQGGSMPTSPGGYLDLSKIGHGAMFKGSKGVLVSSFSN
                     RIIIPGVDEADLTYYKPRTRETLIPPIAHFQHEWTNACKGNLQTTCNFDYAGTMIEQL
                     LLGLVAYRAGTRLEYDGASGRITNNAEANALLSRSYRDGWVLNG"
     gene            5850..6458
                     /locus_tag="PKW60_03825"
     CDS             5850..6458
                     /locus_tag="PKW60_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HPJ98590.1"
                     /translation="MRSNQESARRRIFGCIAAVFLVGCAWFAAHTAVLIWRGLAVDAR
                     NADAIVVLGNKVEQDGTPSPRLQRRLDCAARLHQEGLAPVIIVSGGLGREGHPEAEVM
                     REALIASGVPANAILVDNDGTDTFCTARNAKHMLTERGLESVIIVSSFYHLLRSRMIF
                     ARFGFDEISSAHAPLAWEWRDAYSVVREFPAFYYYRLRSIEK"
     gene            6728..7951
                     /locus_tag="PKW60_03830"
     CDS             6728..7951
                     /locus_tag="PKW60_03830"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(p)ppGpp synthetase"
                     /protein_id="HPJ98591.1"
                     /translation="MRIPQRQKLETEYRNQAEIYGAAARRLLSRLQRLIAAQDINASI
                     KFRVKTFDSYFNKLLRLHRDGASSLIVTDVLGFRIVCPFLSDLDVVQRLLEEHCRVVE
                     AEAKGAEHSFREFGYSSIHLLIDLGNDIKFRPLYATRRVAEIQLRTILQDAWAEVEHE
                     LVYKSQYTLLDEPIKRKLASLNATLTLSDIIFQEIREYQREVQLRENRRKASIERGLP
                     PYRSVPALDEEELTLIQQRDDRPMGLVQHRNELEQLVFEALTAHSSDQFARAIELYSR
                     VLRMKTSRPVRSIIYNHRGMAYLAIAQYPRAEKDFTRAIHFNPDNFRALNNRALACRI
                     RRAYTQALDDLDRSLAINEIQTEAYYIRALTYCDLQDYSKALHDCEYVLNINPGFEIA
                     QHLKELILSQTAKGK"
     gene            8232..>8654
                     /locus_tag="PKW60_03835"
     CDS             8232..>8654
                     /locus_tag="PKW60_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98592.1"
                     /translation="MKAGFASRCITPLLGKEIPGLFERRFAQGVHDDLFVRAAVVDDG
                     EKCIAMVQCDTIKISAGMTARARKAAESLCGIPGRHCFLSATHTHSGGPVFGSFLSEA
                     DAAYQEFTADQAAHAIAEAHRVRQPVVAGTGASLAEGVA"
BASE COUNT         1622 a         2561 c         2796 g         1675 t
ORIGIN      
        1 aaggcgatga gctggatgag atcatccgga aacacggcgg aaaggatctc ctgcccgaaa
       61 aagagactcc gccttcgggg cctcaaccct cccgttctgc tgtgccggag cagccgcatg
      121 acgaagagga gccggacagc ggcggtctgc aaccaggtcc cgaggcgcct gtgccgcacc
      181 ccgcataggc aatttttgca atcagggtgg cggtgccggt tgcaccggca aggcggagaa
      241 catgaccaag ggcgcgacgg aatatctgct ggcggagcgg ctgggttccg gcacgctcgt
      301 catgggtatc gtgaatgtga cccccgattc cttctttgac cggggacgcc atgccacctg
      361 cgaatgcgct gtggcgcatg tccatgaaca ggccgaggcg ggcgccggca tcatcgacat
      421 tggcggtgaa tccacccgcc ccggcgcgga accgctgacg ccggccgctg aattgcagcg
      481 cgtgctgccg gtcgtccgcg cggcggcgcc actgcaggtg ccgctgtcca tcgacacgta
      541 ccacgcctcc acggctgcgg cgtgcattgc cgccggggcc gccatggtga acgacattac
      601 ggcgctccgg ggagatccgg agatggccgg cgtgatcgca tcctctggag tggactgcgt
      661 gctcatgcac atgcagggcg agccgaggac gatgcaagcc aacccgtcgt atacggacgt
      721 ggtggacgat attcgggcat ttttcgatga acggatggcg tatgccgtgg cgcagggcat
      781 tcccgagcag cgcatctggc tggatcccgg atttggattc ggcaagccag tggcgcataa
      841 tctggaattg ctgagacgtc tgaaagaatt gaatgcgctg gggcgcccgc ttctagtcgg
      901 cacgtccaac aagtcgatga ttggcgccgt gctcaacgct ccaatggagg aacgtgacgc
      961 cgggacggcg gcgacagtag ccgtcgccat tatgaatggg gctcgagcgg tccgtgtgca
     1021 taacgtgcgc atgatggtcc gcgttgcgcg catgaccgac gcaatttgtg ggaagtacga
     1081 tccatgagcg aacgattgtt cggaaccgat ggcattcgcg gcattgccaa ccgttatccc
     1141 atgtccgcgg aactggcgct gcaggtcggc cgcgcggtgg gccacatttt ccagcaggag
     1201 gagcgccagc acaccattct gatcggcaag gacacacggc ggtcggggta catgctcgaa
     1261 aatgccctga cggcaggcct gtgttcgatg ggcgtgcatg tcctgctggt cggtcctctt
     1321 cccacgccgg gagtctccta tatcctgcgg agcctgcgct gcgacgcagg catcatgatc
     1381 tccgcgtctc acaatgcgtt ccaatacaac ggcatcaaac tcttcgggcc cgacggatac
     1441 aagcttcctg actccgtgga agacgacatc acccggctga tacaaaacgg cgaggtggaa
     1501 gacattcgcc ccgtcgccga gcgcgtgggt accgcgcgcc gtattgacga cgccgtgggc
     1561 cgctacatcg aattcgccaa atcctgtttc ccgaaaggca tgcgcctgga cgggctgaag
     1621 atcgtgtgcg attgcgcgca cggcgcgtcg tacaaggtgg ggccatctac attttccgaa
     1681 ctgggggccg aggttatcgc gctgggcaat cacccgaacg gaaccaacat caacggcggc
     1741 tgcgggtcga ctcaccccga agacatgcgc gccacggtga tccgtgaacg tgccaacgtg
     1801 gggattgcat ttgacggaga cgccgaccgc atcgtcatgg ccgatgaaga aggccgcctg
     1861 atcgatggca acggcctgct ggccattctc gcctgcgaca tgctcgaaac caaacgtctg
     1921 cccaaaaact gcctcgccac gacgatcatg gccaatggcg gactgctcaa agccatcgag
     1981 ccgctcggag gccaggtggt gtggacgaaa gtgggggacc gctatgtggt cgaggccatg
     2041 cgggagcacg aactggccct gggcggcgaa tcgtcgggcc acgtcgtcat gctggaatac
     2101 aacacgaccg gcgatgcgct gatcgcggcg cttcaggtgc tggccacgat ggttcggcgc
     2161 gatgcgcctc tgtccgtgct ggcgagcgca taccagcaga tgcccgaggc gcatgcccat
     2221 gtgccgcacg acaacggcgc gggaccgtcg gaagaaaaac tggcggagat tgtgcgtgaa
     2281 gcggaagcga aactgaaagg ccggggacgc gtcgtgatcc gggcgtcggg tacggaaccc
     2341 attatccgcg tgatggtgca gcacgagctg cggcgtgaag cagaatcgct tgcgcgggaa
     2401 ctcgcaaaac aagttgcctc gctgtaggta gagggaggtc tcatgattga gctgggagtg
     2461 aatatcgacc acgtggcgac cttgcgtgag gcccggaaag gcgcagcgcc cgacgtgatt
     2521 accgctgcga aactgtgcga actggccgga gcccatcaga tcaccgtgca tctccggcag
     2581 gatcgccggc atatccagga tcgcgacgtg gaattgttga aacaggtgtt gcaggtgcgc
     2641 ctcaatcttg agatggccgc cgccgatgag atcatcgaaa tcgcgcagcg catccgcccg
     2701 gaatttgtct gcctggtgcc cgagaggcgc gaggaagtca ccaccgaagg cggtctggac
     2761 gtggccggcc agattaagcg cctcaccgag gtcaccgcgg gattccatgg cgtcggcgtt
     2821 cgggtcagca tgttcattga ccccgagtat gagcaggtgg atgccagcgc cgagatcggc
     2881 gccgattgcg tggaactgca taccggcgct tacgccagcg cgcgcggtga agaagccctc
     2941 gtggaactgg atcgcattgt cgaggccacc ggccgcgcac tggatgccgg gcttggaatc
     3001 catgcaggtc atggcctttc catccgcaat cttcacgcgg tcgctgcgat tcccggcatc
     3061 agcgaggtca acatcggaca ctccatcgtt gcccgggcca tgttcatcgg gctcgaagaa
     3121 gccgtccgcg agatcctcga catcctcgac gcctatccgg aagaggacta ggccaaaata
     3181 ttcagtatgc cgaaaagcgg tgcggcgccg tcccgagacg tacttgctgc gtggaacaac
     3241 ccggccagga gaacgttgcg gacaaacccc ggtactcagg aattgagcaa tcgggcggcg
     3301 gtttctccgc ggaggttcgg ctgcatttga gctgcggtat ttcccggctg taggaggcgg
     3361 tttcgcattt gctcaagggc ctgctgcgcc gcggcgcggt cgcggatgcg ggagccggcc
     3421 agggcggcag gctgcgcggc cgtctggcgc tgttcgatcg cggccacgaa ttcctcttct
     3481 tttgcgcgga gatccgcctg gatctcgctg agctgcgcgg ccgctttgtc gagtgtctgt
     3541 accgactccg ccgcgtgttc gggagtggaa agatccaggt cgtgaatgcc gagggccgcc
     3601 gcggaggatt caggcagggc gggcagctcg ccgttcacgt cctgccagga gatgccgccg
     3661 gagtcgctgc tgacaagatg ccgccagtgg acggcagtct gcccggccgt ttcatcaaac
     3721 tgcccgagaa gctgccgagt ggtttcgtga agcgcgtagc gctgaacctc cgttcgctca
     3781 ccatcggatg cctgcccggc cagctcccgc atccgcgcga ggctctcctg ctgcttctgc
     3841 aggccgccca gggtcattcg caccgcatgg atcccgtgct gaagactggt tgtctcccgg
     3901 gcggtttgga gcacttcggt tcggcggcgc tcgaggatgc tcagccggat cgcggcctcc
     3961 tcggggcgga ggaggcggtc tccaccgacc gggcgctcct ggccgcgggt ggtatgtccg
     4021 agcgggccac gtgcagcgag gggcgtctca ccggcgcgga ttcgcgtgtc cacagattat
     4081 cccctcctgc gaagcggtat acatcgttct cgcatgggca gcattgtagc atggcggccg
     4141 cgcggcaggc cagccggaac tagctttaat gcgctgcaag ccacgagtct gttcgccggg
     4201 ccctcagccg ttgagcaccc agccgtcccg gtagctgcgg ctgagcaggg cgttggcctc
     4261 ggcgttgttg gtgatgcgcc cgctggcgcc atcgtattcc agccgggttc cggcgcggta
     4321 ggcgacaagc cccagcaaca gctgttcgat catcgtgccg gcataatcaa agttgcaggt
     4381 cgtctgcagg ttgcctttgc aggcgttggt ccactcgtgc tggaagtggg cgatcggcgg
     4441 tatgagcgtc tcccgggtcc gtggcttgta ataggtcaaa tcggcttcat cgacacccgg
     4501 gatgatgatg cggttgctga aactcgagac caggacgcct ttgctgcctt tgaacatggc
     4561 gccgtggccg atcttgctga gatcgaggta gccgccgggt gaggtaggca tcgagccgcc
     4621 ctggtgccag gtgacgcgga tcggcggccg ccaggcattg gccgggtgtt caaatgtggc
     4681 ggtgagctcg accggggtca cctcggggtt gaagggatcg ccggtggctt cggcggaagt
     4741 cggctgttcc gcgtcaatgg cacaccagac gatgtccatc gtatggctgc ccatatcgcc
     4801 ggtctgtcca ctgccgaaat cccagtacat gttccagcag aggcagttgg cccccggggc
     4861 gccttggaaa taaccgggat tgtagggatg gaacggcgac gggccgagcc agaggtcgta
     4921 atgcagggtt ttgggtggtt tgccctccgc gggcaggtag cccggcttgg ggatctgccg
     4981 gttgccccag gcatggactt cttcgagctc accgatgatg ccgtcgcgga tcaattcgct
     5041 tacccgctgg aagttttcgt gggcgtgccg ttgcatgccg cactgcgtgg cgactttgtc
     5101 cttgttcttg aggtacgttt cgcgcacgag ccgggcttcg gccacgctgt tggccagcgg
     5161 cttctcacaa tacacgtgca atccgcggtt catggcccag gtggaaacaa atgcgtgcgt
     5221 gtggtccgtc gtgcagcaga tgatcgcgtc gaggtttttc tgttcgagac atttgcgcca
     5281 gtcgatgtag cgtttggctt tggggaacct ctcggccgcc agggcgatat gatcttcgtt
     5341 gacgtcgcac agcgcgacga cgttttcctc cgcgatgggg tcgtagtgcg ccatcgcgcg
     5401 cccatgcgct ccaattaatg ccacgttgag cttgttgctc ggtgcggccg cacctatacg
     5461 cgttccggag ggtaaaatga ggagcccggt gccggcggct gcggcggctt tcatgaatgt
     5521 gcgcctcgat gctgaagggg cggttgcagt gcggttcttc atggaaggcg gtcctcctct
     5581 gttttgtctt caggcacacg tccgggcggc agaccatatt ttaaagcaca gaggcgctgc
     5641 cgtccatccc ggaagaaaca acgaggccgt ccgttggcgc gggctttggt gctgggaaag
     5701 aaatcggcct tttccgcggc gcttcccctt aaaaatgccc ggaaaccggg tcttcgtacg
     5761 ccctgccgcg tgaggaggcg aatccggcgg aagggaacga gtttcggccc ggggggatac
     5821 acatgatcgg tggagaattt ggaggaattg tgcggtctaa ccaagaatca gcccgtcgac
     5881 ggatattcgg atgcattgcc gcagtttttc tagtgggatg cgcctggttt gccgcccata
     5941 cggcggtact tatctggcgg ggactcgccg tagatgcccg gaacgcggac gccatagtcg
     6001 tgctcggcaa taaggtcgag caggacggca ccccctcgcc aaggctccag cggcggcttg
     6061 actgtgcggc gcggttacat caagaagggc tggcgccggt cattatcgtg agcggcggac
     6121 tcggcagaga gggacatcct gaggccgaag tcatgcggga ggcgcttatt gcgtccgggg
     6181 tgccggcaaa cgcgatcctc gtcgacaacg atggaacaga cacgttttgt acggcccgaa
     6241 acgcaaagca tatgttgacg gagcgcggtc ttgaatccgt gatcatcgtt tcgtcgttct
     6301 atcatttgct gcggtcacgc atgattttcg cccgtttcgg atttgatgag atctcgtcag
     6361 cccatgcccc gctggcctgg gaatggcgcg acgcctattc ggtggtgcgc gagtttccgg
     6421 cgttctatta ttataggttg cggtccattg agaaatgagg acgagcccgg cgaatcgggc
     6481 gggcccaggc tggttcggcg ggctatcatt gcgccggcaa tggcgtttcc ggcacactgc
     6541 atgtatcgta gtagcgccac cgggcggatc ggccggtggc ttttgagcga tggatgccag
     6601 ggagcgggca acagaagcca tgataaaatg ggttctattg tgcgggagca ggggagtgtc
     6661 tgcccgcgga tttccagcgc cagctttgcg aaccgtttcc ccctgaaaag agtgtggagg
     6721 catgccgatg cgcattcccc aacgacagaa actcgaaacc gaataccgca atcaagccga
     6781 gatctatggc gcggcggcgc gccggctgct cagccgtctg caacggctga tcgctgcgca
     6841 ggacatcaat gcctcgatca aattccgggt gaaaacgttc gacagctatt tcaacaaact
     6901 gctacgtctg cacagagacg gcgcatcgag cctcatcgtg acggatgtcc tcgggtttcg
     6961 catcgtgtgc ccgtttctgt ctgatcttga cgtagtgcag cgcttgctcg aggagcactg
     7021 ccgggtggtc gaagccgaag ccaaaggagc ggagcactcc ttccgcgaat ttggctattc
     7081 atcgattcat ctcctgatcg acctgggaaa tgacataaaa ttccggccgc tctacgcgac
     7141 acggcgtgtc gcggaaattc agctccggac cattctgcag gatgcctggg cggaggtgga
     7201 acacgaactg gtgtataagt ctcagtacac cttgctcgat gaacccatca agcgcaaact
     7261 cgcgtcgctc aatgccacgt tgacgctgtc ggacatcatt tttcaggaga tccgcgaata
     7321 ccagcgagaa gtgcagctgc gcgaaaaccg ccggaaagcc agcatcgaac gcggattgcc
     7381 gccgtatcgg agtgtaccgg cgctggacga agaagaactg acgctcatcc agcaacgcga
     7441 tgaccgcccg atgggcctgg ttcaacatcg caatgaactg gaacagctcg tctttgaggc
     7501 cctgaccgct cacagcagcg accagtttgc ccgggcgatc gagctctact cacgcgtgct
     7561 gcgcatgaag accagcaggc cggtgcgttc gatcatctac aatcatcgcg gtatggcgta
     7621 tcttgcgata gcccagtatc cccgggccga gaaagacttc acccgcgcca tccatttcaa
     7681 tcccgacaac ttccgtgcgc tcaacaaccg tgcgctggcc tgccgcatcc ggcgggccta
     7741 tacgcaagct ctcgacgacc tggaccgctc gctcgccatc aatgagatcc agaccgaagc
     7801 ctactacatc cgcgctctga cctattgcga ccttcaggac tactcaaaag ctctgcacga
     7861 ctgcgaatac gtgctcaata tcaaccccgg cttcgaaatc gcccagcatc tcaaggaatt
     7921 gatcctctca cagaccgcga aggggaaata aatcaaccgc aatccggatc ccgccggagc
     7981 aggcggcagg cgggccttcg cccggaagcc tcattgagaa ccccttcctg ccggcggagg
     8041 gcgatacttc ggccagggac tgcctggtga ttgctatggc agccaccggc cttgccgggc
     8101 cattttcagc gcctgtgagt gagccgggcg cttctggcgt cccgcaggag gccgcgttgt
     8161 aattcacccc cgcgtatggt ttactgctcg ccgtacacac aaccctgatc atgttgatgg
     8221 aaggatcttc gatgaaagcc ggttttgctt cgcggtgtat cacccccctt ctgggaaagg
     8281 aaattccggg gcttttcgag cggcgctttg cgcagggggt tcacgacgat ttgtttgttc
     8341 gcgccgccgt ggttgacgat ggcgaaaaat gtattgccat ggtgcaatgc gacaccatca
     8401 agatctcggc cgggatgaca gcccgggccc gaaaggcagc cgaatccctg tgcggcattc
     8461 ccggcaggca ctgtttcctc tcggccacgc acacccattc aggcgggccg gtctttggat
     8521 cgtttctgtc cgaggcggac gccgcctacc aggagttcac cgcggaccag gccgcccacg
     8581 cgattgccga agcccaccgg gtgcggcaac cggtagtcgc ggggaccggc gcaagtctgg
     8641 ccgaaggggt cgcc
//
LOCUS       DAOPBQ010000075         8649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7623_length_8649_cov_4.080987, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000075 DAOPBQ010000000
VERSION     DAOPBQ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7623_length_8649_cov_4.080987"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PKW60_03840"
     CDS             complement(<1..1059)
                     /locus_tag="PKW60_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98593.1"
                     /translation="MKTHQVGIIMNGVTGRMGTNQHLIRSILAIRDQGGVRLSENETI
                     MPAPMLLGRNAAKLERLSEAHGGLPWSTDLDAALSDSQYSIYFETQTTALHYECVKRA
                     IAAGKHVYCEKPIATSSGDACDLYRRAREAGVKHGVVQDKLWLPGLLKMKYLLDTGFF
                     GEVLAVRGDFGYWVFTGEDQPTQRPSWNYRQADGGGIIVDMLCHWRYLVDNLFGNITS
                     VSCLGATHIGRRWDEQGQPYDCDTDDAAYATFITDRNIICQFNSSWCTRVRRDDLLTV
                     HVDGTRGSAVAGLRDCFVQPQSATPKPVWNPDVPSPIDYRSNWMRMPDNQAYNNAFKA
                     EWELFLRHVITDEPFRWDL"
     gene            complement(1114..2478)
                     /locus_tag="PKW60_03845"
     CDS             complement(1114..2478)
                     /locus_tag="PKW60_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPJ98594.1"
                     /translation="MKINFQTTPASLKRALHRFLELSGRKIWALNRAWDPAKGSPVFT
                     RRGKYTARGWTEWTQGFQFGCQLLQFDATGDAAFFAMGRENTVEHMAPHVSHVGVHDH
                     GFNNLSTYGNLRRLAREGRTAETPDALHFYEMALKTSGAVQAARWTTIHDGTGFIHSF
                     NGPHSLFSDTLRSVRSLMLAHTLGHCLMGENDARISLLQRGIEHAANTARYNVYYGEG
                     RDAYDVRGRVVHESIFNTTGGRYRCPSTQQGYSPFTTWTRGLAWVLLGYAEELEFLNT
                     LPDSELDAVGGRAAVRAMMLKAVKAAADFYIENTPTDGVPYWDTGAPGLQQMPRHLQQ
                     ASKPDNPFEPVDSSAAAIAAQGLLRLGHVLGTKNAGKKYYQAGLTVAKTLFAPPYLSE
                     DPKHQGLILHSVYHRPNGWDYVPRGKKTPFGESSMWGDYHAMELAVYLQRESQGGPYL
                     TFFA"
     gene            complement(2654..3667)
                     /locus_tag="PKW60_03850"
     CDS             complement(2654..3667)
                     /locus_tag="PKW60_03850"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPJ98595.1"
                     /translation="MNTTRFVRAATVAAWSICAAVAVNAAVTFDDCFPAQVDPFIGNW
                     TGRWSADEEVEPDIAAQVVALGNNTYQIRLGSRLFMRCPPIGVFEAKRSGDTIPFEHA
                     GIKGEITPDGITGSRGRGKTTFSMTKYIHEPPTLGMTPPENAVVLFDGTNLEQWQEPK
                     GWELLEGGIMMVTPKGESLVSKQSFKSVQLHVEFRLPYLPQDRGQGRGNSGVFVQGVY
                     EVQVLDSFGLEGYYDECGALYKVSAPRVNACLPPLAWQAYDITYTAPKYNENGELTAY
                     PRMTVYHNGILIQDNQEMPQITAWKEVERLAPPPREPGPIMLQAHGNYVQYRNVFLVE
                     VPE"
     gene            3956..6427
                     /locus_tag="PKW60_03855"
     CDS             3956..6427
                     /locus_tag="PKW60_03855"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPJ98596.1"
                     /translation="MPVLFSVRWVCVVLTVLCSATAAFSAEVLLQVEDFEGPWRRQTN
                     ISGYLGTGFCTSNANPQIADTPMTGKAAVPEAGRYAVWVRAYTSENSRRALRAHVNGT
                     PLETTHTSQQRQWSWEKAGEIELQAGEAVVEIWDADDGFESADAVLLTNDLKADPGEA
                     DRRWEVFGGKAPEEADALRFMIEACVAKARLRKDPRDKAVWEERAPGTRQALANALGL
                     DPMPEKTPLNARVTGRAEREDYTIENVVFESRPNFLVTANVFVPRNATFPAPAVVVVP
                     GHAMEEGKNYDLYLRAQIGLVRLGCIVLGYDPIGQGERKLPGFDHTMGYGSLLVGETN
                     EGMITWDTIRAVDYLLTRSDVDPGRLGLTGNSGGGENTFYAMPMEERFAAAASFCFVC
                     SYEDWIRDGGNHCICNHLPGILNHMEEFEILALNAPRPFLAGNGAEDKIFPVDGTRMT
                     CERARRIYRMYGAADRMVNIDVPMGHGWSQPLREAGYGWMGKWLLGVGDGSPISEEAF
                     EGEDPASPDLLCFDGNRIPTDSETVVSLNQKRAETLRAAYTTPPTDSAVWREQAEAMR
                     EAIWEVFGARPADFTPEVRTLDTFDWDGRRIDTLAITTEPGMTVAALLLRSATTEGKA
                     PAVIFLGETDKQEVRGDARAQELLDDGYCVLALDTRGMGETAGKYPQNQIVSNGVHLG
                     RPLFAQRVWDVLQAAHYLATRPDIAPDRLGCIGLGPSGVLALYAAALSDRFEEVEAVR
                     PLASFRYFIENDQPQPLWLCVPNILKVADVAQAAALAAPAAVTIAGAVGYGKEPLEPA
                     AAHQEFSYATSVHGFAGSPARFTLR"
     gene            6467..8206
                     /locus_tag="PKW60_03860"
     CDS             6467..8206
                     /locus_tag="PKW60_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPJ98597.1"
                     /translation="MTEFSNLDIVVIAGYFIALVVLGFAVGRTQERSSEDYFLAGRSL
                     PWYVVGASFIGSNISTEHFIGMIAAAYTYGICVAQWEWGNVFAFSVLVWFFIPFLLSS
                     RVFTAPEFLERRYNSACRLMFAVLTVVANITAFLAAVLYASAIGLKAVFAWPDTVAIA
                     GVNVSTLVLGVVGMGVVAGSYAIYGGLRSVAWTDVFQVVVMVIGGILVTLLGLSYLGQ
                     GEGLLAGWSTMIARNLGDGAAWKAAIDGAAQEMVGRDHYNRLQAFQPFSHKLVPWQGV
                     VLSWLSVSIWYNCINQFMIQRVFAAKNDWHARMGIIFAGFMKIFMPFIVVIPGLLYFA
                     MEPGITDAQQVDQTYAILVTKLLHSGMRGLLLAALFGAIQSTIDSVLNSTSTIITLDI
                     HHKFINPGASEESLVRMGKWIGASVLLLAMVIAPFIGWLGQGVFYYIQNLYAYFAPPF
                     AAVFLVGILWKRATPVAATITIPAGILFGFFLDFVVFRYWLASGTAFTLRSAYNWIFC
                     VVLLVFASLLSTPPPPEKITDEVSINWRSLAAFKDLGPSWYRNVGLWWALFVAGILAC
                     YAAFSGLFLAPRG"
     gene            complement(8314..>8649)
                     /locus_tag="PKW60_03865"
     CDS             complement(8314..>8649)
                     /locus_tag="PKW60_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPJ98598.1"
                     /translation="KWLLEEIGGLDKMYALNQQKAKLLYDAIDASSGFYRGHALPECR
                     SIMNVTFRLPSEELEGKFIKEATANGLAALKGHRSVGGCRASIYNAMTLEGVTALRDF
                     MLEFMASNG"
BASE COUNT         1499 a         2586 c         2817 g         1747 t
ORIGIN      
        1 gagatcccac cggaacggct cgtctgtgat cacatgccgc agaaacagct cccattccgc
       61 cttgaacgca ttgttgtagg cctggttgtc gggcatccgc atccaatttg agcgataatc
      121 gatgggactg gggacatcgg gattccatac gggcttcggc gtcgccgatt gcggttgcac
      181 gaagcagtcg cgcagccccg ccaccgccga tccccgggtg ccgtcgacat gcacggtcag
      241 caggtcgtcg cgccgcaccc gcgtgcacca tgaggagttg aactggcaga tgatgttccg
      301 gtcggtgatg aaggtggcgt aggccgcatc gtccgtatcg cagtcgtacg gctggccctg
      361 ctcgtcccag cgccggccaa tgtgcgtcgc gcccaggcag ctcacggagg tgatgttgcc
      421 gaaaaggtta tcgaccagat agcgccaatg gcagagcatg tcgacgatga tgcctccacc
      481 gtccgcctgg cggtagttcc aggacgggcg ctgtgtgggc tggtcttcgc ctgtgaacac
      541 ccagtaaccg aaatcgccgc gaacagccag cacctcgccg aaaaatccgg tatcgagcag
      601 gtatttcatt ttgagaagcc cgggcagcca caacttgtcc tgcaccaccc cgtgtttcac
      661 gcccgcttcc cgggcccggc ggtagagatc gcaggcgtct ccggaacttg tggctatggg
      721 tttttcgcaa taaacgtgtt ttcccgcggc gatggcccgt ttcacgcatt cgtaatgcag
      781 cgccgtggtc tgcgtttcaa aatagatgga gtactgactg tccgagaggg ccgcgtccag
      841 gtcggtcgac cagggcagtc cgccgtgtgc ctccgacaac cgttcaagct tcgccgcatt
      901 ccgccccagc agcatggggg caggcatgat cgtttcattt tcgctgagcc ggaccccgcc
      961 ctgatcgcgg atagcgagga tcgaccggat caggtgctga ttcgtgccca tgcgtcccgt
     1021 gacgccgttc atgatgatgc ccacctggtg cgttttcatc gcaatatcct ctctgttgcg
     1081 ttccccgcag cggaacggcc gctcccggcc ccgctacgca aaaaatgtca gatacggccc
     1141 gccttggctc tcacgctgaa ggtacaccgc gagttccatg gcgtggtagt cgccccacat
     1201 ggacgactcg ccgaacggcg tcttttttcc ccggggcacg taatcccacc cgttggggcg
     1261 gtgatacacc gaatgcagga tcaacccctg gtgtttcggg tcctcggaaa gatagggcgg
     1321 ggcaaacagc gttttcgcga cggtcaagcc cgcctggtaa tacttctttc ccgcattttt
     1381 ggtgccgagc acgtgcccca gccgcagcag tccctgcgcg gcgatcgctg ccgcggagct
     1441 gtccacgggt tcgaacgggt tgtccggttt cgaggcctgc tggagatgcc ggggcatctg
     1501 ctgcagcccc ggcgccccgg tatcccaata cggcacgccg tccgtggggg tgttctcgat
     1561 gtagaagtcg gcggccgcct tgaccgcctt gagcatcatc gcgcgcaccg cagcgcggcc
     1621 tccaacagcg tccagctcac tatcgggcag ggtattcaga aactcgagct cttccgcata
     1681 gcccaggagc acccaggcca gcccccgcgt ccacgtcgtg aatggcgaat acccctgctg
     1741 cgtgcttggg caccggtacc ggccgccggt tgtgttgaaa atgctttcgt gcacgacccg
     1801 gccccgcacg tcgtacgcgt cgcggccttc gccatagtag acattgtatc gggccgtgtt
     1861 cgcggcatgc tcgatgccgc gctggagcag cgaaatgcgc gcgtcattct cgcccatgag
     1921 gcaatgaccc agcgtatggg ccagcatgag cgaacgcacc gaccggagcg tatcgctgaa
     1981 cagcgagtgc gggccgttaa aggagtggat gaaacccgtg ccgtcgtgga tcgtggtcca
     2041 tcgcgcggcc tgcaccgccc ccgatgtttt caaggccatc tcgtagaagt gcagcgcgtc
     2101 gggtgtttcg gccgtacggc cttcccgggc caggcggcgc aggttcccgt atgtcgacag
     2161 gttgttgaac ccgtgatcgt gcacccccac gtggctcaca tgcggggcca tgtgttctac
     2221 agtgttttca cggcccatgg caaaaaaagc cgcgtcgccc gtcgcgtcga actgcagaag
     2281 ctggcacccg aactggaatc cctgggtcca ttccgtccat ccccgcgcgg tgtattttcc
     2341 acgccgcgtg aacaccggag agcccttcgc cgggtcccag gcccggttga gcgcccatat
     2401 cttccggccc gacagctcca gaaaccggtg cagcgcccgc ttcaggctgg cgggcgtcgt
     2461 ctgaaaattg attttcacgt ttgcgtcccc gttttcctgt gagaacactt cccgtgctct
     2521 gggtttagtt ccggtacgct tgcatgatag cgaatgcgtt ccgcgctcga caattcggcg
     2581 gcgaaacgaa gcccgtgccg gccgccggga accatttccg ctgcgcgcag cgccggctgt
     2641 gcctgggcgg ggcttattcc ggcacttcta cgagaaacac gttgcgatac tggacataat
     2701 tgccgtgggc ctgaagcatg atggggcccg gttcgcgcgg cggcggcgcc agacgctcca
     2761 cttctttcca ggcggtgatc tgcggcatct cctggttgtc ctggatcaga atgccgttgt
     2821 gataaacggt catgcgcgga taggccgtca attcgccgtt ttcgttgtat ttcggggcgg
     2881 tgtaggtgat gtcgtacgct tgccaggcga gcggcggcag gcacgcgttc acgcgcggcg
     2941 cggacacctt gtacagcgcg ccgcattcgt cgtaatagcc ttcgaggccg aaactgtcga
     3001 gtacctgcac ttcgtacacg ccctgcacaa ataccccgct gtttccgcgg ccctggcccc
     3061 ggtcttgcgg caaataggga aggcggaact cgacgtgaag ctgcacgctc ttaaacgact
     3121 gtttggagac gagcgactcg cccttgggcg tcaccatcat gatgccgcct tccagcagct
     3181 cccatccttt gggttcctgc cattgttcga ggttcgtgcc gtcaaacagc accacggcgt
     3241 tttccggcgg cgtcatgccg agcgtcggcg gctcatggat gtatttcgtc atggaaaacg
     3301 tggtcttgcc acgccctcgc gaccccgtaa tgccgtccgg agtaatctcg cctttgatac
     3361 ccgcatgctc aaagggaatc gtgtcaccgg agcgtttcgc ttcgaatacg cctatcggcg
     3421 gacagcgcat gaacagcctc gagcccagcc gaatctgata ggtgttattg cccagagcga
     3481 ccacctgggc tgcgatatcc ggttcaactt cctcgtccgc cgaccagcgg ccggtccaat
     3541 tgccgataaa agggtctacc tgagccggaa agcagtcatc aaaggtgacg gctgcattca
     3601 cggcgacagc cgcacagatg ctccatgccg ctacggtcgc cgcgcgcaca aatcttgtcg
     3661 tgttcatggg atgcgccctt ccataatccg ttagcctgcc ggcgccccgg cgccgttgct
     3721 gcgccagtct ctccggtgtg atcgatgcgg gtgattttat ggtagcagac cccggcgcat
     3781 cttgtcattg cggaaaaaac tcagaatcgc gcacgttggc ggcatgtttg gcgccgcctc
     3841 tctgctggac gcaagcagcc aatgcaaaca gcgggcggtt tactcgttcg cgcgaaaaag
     3901 gctaccatgg gtttttccag agccggggtg cgttcggaaa gggggaaatg aagggatgcc
     3961 ggtgcttttt tctgtgcgat gggtctgtgt ggtcttgacg gtgctgtgct cggctactgc
     4021 ggcattcagt gcggaggtgt tgctgcaggt ggaggacttt gaagggccgt ggcggcggca
     4081 gaccaatatt tcgggctatc tgggcacggg gttctgcacg tcgaatgcca atccacagat
     4141 cgcggatacc ccgatgacgg gcaaggcggc tgtgccggag gcaggccggt acgcggtgtg
     4201 ggtgcgggcc tatacgtcgg agaattccag gcgggctctc cgggcgcacg tgaacggtac
     4261 gccgctggaa acgacgcaca cgagccagca acggcaatgg agctgggaaa aagccggaga
     4321 aatcgagctg caggcaggag aggcagtcgt ggaaatctgg gatgcggacg acgggttcga
     4381 atcggcggat gcggtgctgc tcacgaacga cttgaaggcg gatcccggtg aagcggatcg
     4441 ccgctgggaa gtgttcggcg gcaaggcccc ggaagaggcc gatgcgctgc ggttcatgat
     4501 tgaggcctgc gtcgcgaagg cgcggctgcg caaagacccg agagacaagg cggtgtggga
     4561 agaacgcgcg ccgggcacga gacaggcgct ggcgaatgcg ctcgggctgg atccgatgcc
     4621 ggagaaaaca ccgttgaacg cccgggtgac gggccgggcg gagcgcgaag actatacgat
     4681 tgaaaacgtg gtgttcgaga gccggccgaa ctttctcgtg acggccaacg tgttcgttcc
     4741 ccgcaacgcc acgtttcccg cgccggccgt ggtcgtcgtg ccggggcatg ccatggagga
     4801 aggcaagaat tacgacctct acctgcgcgc acagatcggg ctggtccggt tgggctgcat
     4861 tgtgctgggc tacgacccga ttggccaggg cgagcgcaaa ttgccgggtt tcgaccacac
     4921 gatgggctac ggctcgctcc tcgtgggtga gaccaacgag ggaatgatca cctgggacac
     4981 gatccgggcg gtggactacc tgttgacgcg ctcggacgtc gatcccggcc ggctgggcct
     5041 gacaggcaat tccggcggag gcgaaaacac gttctacgcg atgccgatgg aggaacggtt
     5101 tgccgcggcc gcctcgttct gtttcgtctg ctcatacgag gactggattc gggacggagg
     5161 caatcactgc atctgcaacc acctgccggg catcctgaac cacatggagg agtttgagat
     5221 cctcgcgttg aacgcgccgc gccccttcct ggcgggcaat ggcgctgaag acaagatatt
     5281 ccctgttgac ggaacgcgga tgacctgtga gcgtgcccgc cgcatttacc ggatgtacgg
     5341 ggcggcggac cggatggtca atatcgacgt gccaatgggc cacggctggt cgcaaccgct
     5401 ccgggaagcg ggatacggct ggatgggcaa gtggctgctc ggcgtgggcg acggcagccc
     5461 catcagcgag gaggctttcg agggcgaaga tccggcctcg ccggatctgc tgtgcttcga
     5521 cggcaaccgg atccccacag attcagagac agtggtttcg ttgaaccaga agcgcgcgga
     5581 gaccttgcgg gccgcgtaca cgaccccgcc cacggacagc gcggtctgga gggaacaggc
     5641 ggaagccatg cgcgaggcta tctgggaggt ttttggcgcg cggcccgcgg atttcacccc
     5701 ggaggtccgc acgctggaca cgtttgactg ggacgggcgc cgcatcgaca cgctcgcgat
     5761 cactacggag ccgggcatga cggtggccgc gttgctgctg cggtccgcca cgacagaagg
     5821 caaagcgccg gccgtgattt tcctgggcga aaccgataaa caggaggtgc ggggagatgc
     5881 gcgtgcgcaa gagctgctgg acgacggcta ctgtgtgctt gcgctcgaca cgagaggcat
     5941 gggcgaaacc gccggcaagt atccgcagaa ccagatcgtg agcaacgggg tccatctggg
     6001 gcggccgttg tttgcgcagc gggtatggga cgtgctccag gcggcgcatt acctcgcaac
     6061 gcgccccgat atcgctccgg atcggcttgg ctgcatcggg ctgggtccgt ccggtgttct
     6121 tgcgctgtat gcggcggcgt tgagcgacag gtttgaggag gtcgaggcgg tgcgaccgct
     6181 ggcgagcttc cgctatttca tcgagaatga tcagccgcaa ccgctgtggc tgtgtgtgcc
     6241 gaacatcctg aaggtggcgg atgtggcgca ggcggccgcg ctggccgccc cggccgccgt
     6301 cacaattgcg ggcgcggttg gatacgggaa agagccgctc gagccggccg ctgcgcatca
     6361 ggaattttcg tatgccacaa gcgtgcacgg gttcgccggc tccccggcgc ggtttacgct
     6421 gcggtaagaa cgtaatggcc cgcgcggcaa acggaaaggt tgtggcatga ccgaattttc
     6481 gaacctcgat atcgtggtga ttgccggtta tttcatcgcg ctggtagtgc tgggatttgc
     6541 cgtcggaaga acgcaagagc gctcttcaga ggattacttt ctggccgggc gcagcctgcc
     6601 gtggtatgtg gtcggggctt cgtttatcgg gtcgaatatt tcaaccgaac atttcatcgg
     6661 catgatcgcg gcggcctaca cctacggcat ctgcgtggcc cagtgggaat ggggcaatgt
     6721 tttcgcgttt tcggtgctgg tgtggttttt catccccttt ctgttgagca gccgggtatt
     6781 caccgcgccg gagttcctgg aacggcgcta caacagcgcg tgccggctga tgttcgccgt
     6841 actgacggtc gtggcgaaca ttaccgcgtt cctggcggcc gtgctgtacg catcggcgat
     6901 cgggttgaag gccgtgtttg catggcccga cacggtggcc attgcgggag tgaatgtttc
     6961 gaccctcgtg ctgggcgtcg tgggtatggg cgtggtggcc ggcagttatg ccatctacgg
     7021 cgggcttcgc tccgtcgcat ggaccgacgt gttccaggtg gtcgtgatgg tcatcggagg
     7081 catcctggtg acgctgctgg ggctatccta cctgggccag ggggaaggcc tgctcgcggg
     7141 ctggagcacc atgatcgccc gcaatctggg cgacggcgcc gcctggaagg ccgccataga
     7201 cggagcggcg caggagatgg tcgggcggga tcactataac cggctccagg cctttcagcc
     7261 gttttcgcac aagctcgtgc cctggcaggg cgtcgtgctg agctggctct cggtgagcat
     7321 ctggtacaac tgcatcaacc agttcatgat ccagcgcgtg ttcgcggcaa agaatgactg
     7381 gcacgcccgc atgggcatca ttttcgccgg tttcatgaag atcttcatgc cgttcatcgt
     7441 ggtgattccg ggcttgctct actttgccat ggagcccggg atcaccgatg cgcagcaggt
     7501 ggaccagacc tatgcgatcc tcgttacgaa actgctgcat tcgggaatgc gcggcctgct
     7561 gctggcggcg cttttcggag cgattcaatc gaccatcgat tcggtgctca attcgacatc
     7621 aaccatcatc acgctcgata tccaccacaa attcatcaac cccggcgcct cggaagaaag
     7681 cctggtacgt atgggcaaat ggattggcgc gtcagtgctc ttgctcgcca tggtgatcgc
     7741 gccgttcatc ggatggctcg ggcagggtgt gttttactac atccagaacc tgtacgcgta
     7801 tttcgcgccg cccttcgccg cggtgtttct ggtcggcatc ctctggaaac gggcgacccc
     7861 ggttgcggcc acgatcacca tccccgcggg gatcctcttc gggttctttc tggactttgt
     7921 ggtcttccgg tactggctcg ccagcgggac cgcgtttacg ctgcgttcgg catacaactg
     7981 gatcttctgt gtggtgctgc tggtgtttgc gagcctgctg tcgacgccac cgccgccgga
     8041 aaagatcacg gacgaggtga gcatcaactg gcgcagcctc gccgcgttca aagacctggg
     8101 gccgtcctgg taccgcaatg tgggcctgtg gtgggcgctg ttcgtggcgg ggattctcgc
     8161 ctgttatgcg gcgttcagcg gcctgttcct ggctccgcgc ggctgagccg ctctcccggg
     8221 cgcacccggt ccgttgaatg ggctggatgc aacgcgcgcg cggagcctga ggctgccgcg
     8281 cgcgtgcagt cgaaaacggg tttgtccagg tgctcagccg ttgctggcca tgaactcaag
     8341 catgaagtcc cgaagcgccg tcacgccttc gagggtcatc gcgttgtaga tggaagccct
     8401 gcaaccgcca accgatcggt ggcccttcaa tgcggcgagc ccgttggccg tggcctcctt
     8461 gatgaacttg ccttcgagtt cttcactggg caggcggaac gtgacgttca tgatggaccg
     8521 gcattcgggg agggcgtggc cccggtagaa cccggagctc gcgtcgatgg cgtcgtagag
     8581 cagcttggct ttctgctggt tgagcgcgta catcttatcg agcccgccga tttcctcgag
     8641 cagccattt
//
LOCUS       DAOPBQ010000076         8641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7629_length_8641_cov_3.527137, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000076 DAOPBQ010000000
VERSION     DAOPBQ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7629_length_8641_cov_3.527137"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="PKW60_03870"
     CDS             <1..1382
                     /locus_tag="PKW60_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPJ98599.1"
                     /translation="AFSALAQQPAMQSAQTDISQQSDISITAYNSGIALVRDVRKLDL
                     PQGEIELKFMDVAQQIRPETVGLRSLAQAGSVAILEQNYEYDLISPEKLMEKYIGKEV
                     KLINYSNEIGFLEKSAELLSINQGPVYRVDGQIFLGHPGNVVVPEIPDNLIAKPSLIW
                     HLNNSRGAQQLEATYITNGVSWKADYVLTLAKTDATLDVAGWVTLENQSGASYENARL
                     KLVAGDVNIAQPQMMGKRMMEAAMVMAAAPPEMAREETFAEYHLYTIPRRTTIKQNQS
                     KQVALLSGSGVQCEKRYEFRGMLDFYSNRFPPMRDQHVDVFLKFQNSEANRLGIPIPA
                     GVMRIYQEDSEGMLQFAGEDNIQHTPKDEEIRLRMGKAFDVVADRVQKNYSKLSDRAH
                     EAEFEITVRNHKALDVVVDIVEPMPADWKIVSKSHDFVKKDAHTAVFSVPVPKDGKVA
                     VTYRVQVQY"
     gene            complement(1433..>2347)
                     /locus_tag="PKW60_03875"
     CDS             complement(1433..>2347)
                     /locus_tag="PKW60_03875"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPJ98600.1"
                     /translation="LWQERKALQKFATVKPDAHGRGSRLLPFLLIWALPMSEEAVPAP
                     LHWFGDGSNPVALFRSAWDDNATYVGIKGGSPSANHGHMDAGSFVLEMDGVRWAVDLG
                     ADGYHGLESKGVDLWNRRQDSQRWTVFRLNNFSHNTLVVDGELQQVKGLAPITAFSDE
                     PDAPYALIDLAPVYEGQLAGARRGVRLVGPSVLVQDEVRALDRTSMVRWGMVTRAEVT
                     LVDDTTAQLKQNERTLWLRALAPGNTRLRLFETENPPRDYDSRNPNTRMIGFESEVGA
                     GAQATLAVFFQSGEAPGEAPALIPLDEW"
     assembly_gap    2348..2357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2358..3349)
                     /locus_tag="PKW60_03880"
     CDS             complement(<2358..3349)
                     /locus_tag="PKW60_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase"
                     /protein_id="HPJ98601.1"
                     /translation="MQSEDGLVWAILAVWAIGALMAPPSLAASVEEELAERIENVAPV
                     HPRLFFTESEETEIRERFESDPLLQAVFTYIRASSDILLEQPPLEREKIGRRLLSVSR
                     YCLQRVEYLAFTYRMTRHEPYLRRAEEEMLAAASFEDWNPSHFLDVAEMTAALAIGYD
                     WLYNDLSPETRSAVKAALLDKGLRPSMAVTRGWVNGNNNWNQVCHGGSVMGALALLED
                     EPELAVQITARAIANVPRAMHEYEPDGVYPEGPSYWEYGTSYNVLLIAALESVFAHDF
                     GLGSAPGFLKTPEFYLRATGPSGLYFNFSDCGERGEISPAMHWLARRRQDPSLL"
     gene            3901..5148
                     /locus_tag="PKW60_03885"
     CDS             3901..5148
                     /locus_tag="PKW60_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98602.1"
                     /translation="MEAKEKNLVKRAGLLCSVWLAVATAVFAASDDSNAVRESIDTLI
                     LLGDYGLIERYDSSFTSRGQGKIPGAGERMVVGAANLSWNNDALLVDDDLEGQILECR
                     FEDMDMKRGTVPQLPEGPGFRGRWHGVTVILPLADGQIYVRDEGYARLPELGKCPPTI
                     HIDLRSGEARVLNTFGIGGLFDIRTAPAGDRFVLLGKGIAVMVDAMTGHVLREVAHLA
                     EYRRSLYQEPEAGKVYLLGYEVDWEQEALDCWWISMAPENAGEVEHVLVSMKTGERLK
                     QTSMSPWPELPDFDRFEFPKTFLGSVLSKRKLVSGRREMTGNRPLLRLVEAHTLARLS
                     ARPALLENLPSEKLVTISISPQGRFLLGEYFTTVEERTWEDPPEHSRRIVILDTETGA
                     IEADFVFKNPVAGVFFLGTHENG"
     gene            5689..6372
                     /locus_tag="PKW60_03890"
     CDS             5689..6372
                     /locus_tag="PKW60_03890"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPJ98603.1"
                     /translation="MPEIPAYQIQRTASQPEFNGAWDGPLWRSVPTVSIAHFHPASSD
                     HRPVTHAKLLYDADALYAIFRVEDQYVRATREDLNSSVCNDACVEFFFEPRAGGGYFN
                     FEMNCLGTLHAGYVEDPTRTPEGLGKATRLLRRQAAMMRVYHSIPSVVFPERADPVRW
                     VVEYSIPFALLEEFTGPLAGPAGQTWRGNFYKCAEDNSHPHWASWAPIGEQLNFHVPE
                     YFAPLHFAG"
     gene            6494..>8641
                     /locus_tag="PKW60_03895"
     CDS             6494..>8641
                     /locus_tag="PKW60_03895"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPJ98604.1"
                     /translation="MLRLALTLAIAAVLPIASAEEAAVQLYVAPQGNDAWSGKLPEPN
                     ADGTDGPLATLTAARDRLREMKGSQRLPGPVSVQLREGHYRITEAIVFEPLDSGLPTA
                     PITYEAYPGEHPVISGGVPVTGWTQEGPAWKAQVPEAGPRPAPFCALWVNGEFRGPAR
                     SPNDGEFVSDGIAPEIKDPATDKPVISQHNAIRYKEGDVQEWHNLEDVLVVGLHSWDI
                     TILPVDRIDRGERLLIMKRPEGGWNFNQWGGTQQRYYIENILEGLDAPGEWYLDRKAK
                     TVLYLPRPGENMNAVEVIAPVARQLIVLQGDAANGEFVCHLHFKGIAFHHTDYSLPPE
                     GMNSQQAASSVHAAIQGRAARYCSIEDCEVAHISNYAIWFGEGCQDNRIVKNHIHDLG
                     AGGVRLGEMGDAPTTNHVCRLNTVDNNWIHDGGTIYHPAVGVWLGRTSYNTVSHNEIH
                     DFFYTGVSVGWSWGYAESSANHNIIEYNHIHHLGKRYLSDMGGIYLLGRAPGTECRYN
                     HIHDVFSFDYGGWGIYPDEGSSYLRIENNVVYNTKTGGFHQHYGQENRVENNIFAYAH
                     EGQIQRTRMEDHLSFTFRRNIVYFNNGWLLSSNWRDNQYDLDFNCYWDETVGSVLFAG
                     HSLERWREKGQDRHSIVADPLFENPHAFDFRLKPESPALALGFEPIDMSGIGLYGDEA
                     WVQAPGQIQHPPSELPAPFKPKFVHDDFEGTPAG"
BASE COUNT         1824 a         2504 c         2585 g         1718 t
ORIGIN      
        1 gggccttcag tgcgctggcc cagcaaccag ccatgcagtc ggcccagacg gatatcagcc
       61 agcaatccga catctcgatc acggcctaca acagcggaat agccctcgtg cgcgacgtcc
      121 gcaaactcga cctccctcag ggtgaaatcg agttgaaatt catggatgtg gcccagcaga
      181 tccggcccga aaccgtaggg cttcgttcgc tggcccaggc gggttcggtg gccattcttg
      241 agcaaaacta cgaatacgac ctgatcagcc ccgaaaaact gatggaaaaa tacatcggca
      301 aggaagtcaa actcatcaat tacagcaatg agatcggttt tctcgaaaaa tccgctgaat
      361 tgttgagcat caaccagggg ccggtatacc gcgtcgacgg gcagatcttt ctggggcacc
      421 ccggcaacgt cgtcgtgccg gaaatccccg acaacctcat cgcgaaacca tcgctcatct
      481 ggcacctcaa caacagccgc ggcgcacagc agctggaggc gacctatatc accaacggcg
      541 tctcctggaa ggccgattac gtcctaaccc tcgccaaaac cgacgccacc cttgatgttg
      601 cgggctgggt caccctggag aatcagtcgg gcgccagcta cgaaaatgcc cggctcaagc
      661 tggtggccgg cgacgtgaat atcgcccagc cgcagatgat gggaaaaagg atgatggaag
      721 cggcgatggt catggcagct gccccgcccg agatggcgcg cgaggagact ttcgctgagt
      781 accacctgta cacgatcccc cggcgcacca ccatcaagca aaatcagtcc aagcaggtcg
      841 ccctgctcag cggttccggc gtgcagtgcg aaaaacgtta tgaattccgc ggaatgctcg
      901 acttctacag caaccgcttc ccgcccatga gagaccagca cgtggatgtc tttctcaagt
      961 ttcagaatag cgaagcgaac cgcctcggca ttccaatccc ggcgggcgtc atgcggatct
     1021 atcaggaaga cagcgaaggc atgcttcagt tcgcgggcga ggacaacatt cagcacacgc
     1081 ccaaggacga ggaaatccgc ttgcgcatgg gcaaggcgtt cgacgtagtt gccgatcgtg
     1141 tccagaagaa ttactcgaag ctgtcggacc gcgcccacga agcagagttc gagatcaccg
     1201 tccgcaatca caaggcgctt gacgtcgtgg tcgacatcgt cgaaccgatg cccgcggact
     1261 ggaagatcgt gagcaagtcc catgatttcg tgaaaaaaga cgcccatacg gccgtcttca
     1321 gcgtgcccgt gcccaaggat ggcaaggtcg ccgtcaccta ccgggtccag gtgcaatact
     1381 agcgattcgg accggcccgc gggagtccct cgagacttcc gcgggctttt ttttaccact
     1441 cgtccaatgg gatcagcgca ggcgcctcgc cgggcgcttc acccgactgg aaaaagaccg
     1501 ccagggtagc ttgcgccccc gctccgacct cggattcgaa tccgatcatg cgggtgttgg
     1561 gattgcggct gtcgtagtcc cgaggcgggt tttccgtctc aaacagcctc agccgggtgt
     1621 tgccgggcgc cagggcgcga agccagaggg tgcgttcatt ttgtttgagt tgggcggtgg
     1681 tatcgtccac aagcgtcacc tcagcccgcg tgaccatgcc ccagcgcacc atactggttc
     1741 gatccagtgc gcggacttca tcctgcacca ggaccgaagg tcccacgagc cgtacgccgc
     1801 gccttgcccc cgcaagctgg ccctcgtata cgggcgccag gtcgatgagc gcatatgggg
     1861 catctggctc atccgagaag gcggtgatgg gcgccaggcc tttgacctgc tgcagttcgc
     1921 catcgaccac gagggtattg tgactgaagt tgttcagccg gaacaccgtc cagcgctggc
     1981 tgtcctgtct tcggttccag aggtcaaccc ctttggattc caggccgtga tagccatccg
     2041 ccccgaggtc aacggcccat cggaccccat ccatttcaag cacaaacgag cccgcatcca
     2101 tgtggccgtg attcgcgctg ggtgacccgc ctttaatgcc tacatacgtc gcgttgtcgt
     2161 cccaggcgct gcggaagagc gccacggggt tggagccatc gccaaaccag tgcagcggcg
     2221 caggaacggc ctcttcgctc atgggaagcg cccaaatcag cagaaacggc agcaagcggc
     2281 tgccccggcc atgggcatct ggtttgactg tggcaaattt ctgcagcgct ttgcgctcct
     2341 gccagagnnn nnnnnnncag agcaggctgg gatcctgccg tcgccgtgca agccagtgca
     2401 tggccggaga gatttccccg cgctccccgc agtccgaaaa attgaagtac agccccgagg
     2461 gcccggtcgc gcggaggtag aattcaggcg ttttcaggaa ccccggcgct gaccccaggc
     2521 caaaatcgtg ggcgaatacg gattccagcg ctgcaatgag caaaacgttg tatgaggtgc
     2581 cgtattccca gtaactcggc ccttcggggt acacgccgtc cggctcgtat tcatgcatcg
     2641 cgcgcggcac gttggcgatc gcgcgggcgg tgatctggac cgcgagttcc ggctcgtcct
     2701 ccagcaaagc caaagcaccc atcacggaac ccccatggca gacctggttc cagttgttgt
     2761 ttccgttgac ccacccccgg gtaaccgcca tggacgggcg caggcctttg tcgagcaggg
     2821 cagcctttac ggccgatcgc gtttccgggg acaagtcgtt gtataaccag tcgtatccga
     2881 tagccagtgc tgcggtcatt tcagccacat caagaaaatg gctgggattc cagtcttcga
     2941 acgatgcggc ggcaagcatt tcctcttcgg cccgccgcag gtaaggttca tgcctggtca
     3001 tgcggtaggt gaacgccaga tattccaccc gctgcaggca ataccgggag acgctcagca
     3061 gccgccggcc gatcttctcc cgttcgagcg ggggctgctc cagcaggatg tccgaactgg
     3121 ctcggatgta tgtgaacacg gcctgaagaa gcggatcgga ttcaaaccgc tcacggattt
     3181 ccgtctcttc ggattccgtg aaaaaaaggc gcggatgcac gggagctacg ttctcaatcc
     3241 gttccgcaag ctcctcctcg accgaggcgg ccaacgacgg cggcgccatg agtgctccga
     3301 tcgcccatac agccaggatt gcccaaacaa gaccgtcttc agattgcaca tgccgtttcc
     3361 tcaactgtgt ttgcccccgc aaccatcgca agcgtccact gccgattgta tcggctggcc
     3421 acatcatggg caacccgggg ctcttccagg acctccctcc ttcccaaatc cgcccgcata
     3481 tgccatactg cattcgtacc agataggggt ctgtgtagat gccggataca accgccatag
     3541 ccgtgaaaga acacgtcagc caggccgtgg gcggcacggc cacaaggcac gggtgagata
     3601 cgggctagac ggtgctgaat gaggagggca agctcagtgc cctcaagcat acgtacacca
     3661 gccgtaaggc atttgatccg ccggataagg cgtatttctg ctggtattct tggcggggcg
     3721 acgcatgcgc cgcccggcac gaatggtatg ctggtgtcaa ggatggcgca ttagtcgagc
     3781 atgatcgcga acctggccgt gtagtgcggc acgtcaatga ctgttgggat tattacgtgg
     3841 agcgcccatg ggagtaggga ctctagtgcg gcgggctggt tgtgaaggga gattgagatc
     3901 atggaggcga aagagaagaa tctcgtgaaa cgagcgggtc tcctgtgcag tgtttggctt
     3961 gcggtggcga cagcggtatt cgcggccagt gacgacagca acgctgtcag agagagcatc
     4021 gacaccctta ttctcctcgg tgattacgga ttgattgagc gatacgattc ctcattcaca
     4081 agtcgaggcc agggaaagat cccgggagca ggtgaaagaa tggtagtcgg cgccgcgaac
     4141 ctctcctgga acaacgacgc attacttgta gatgatgatc tcgagggcca gatattggaa
     4201 tgccgttttg aagatatgga tatgaagcgg ggcacagtgc cgcagttgcc ggaagggccg
     4261 gggttccgtg gcaggtggca tggtgtaacg gttattcttc ctcttgccga cggtcagatt
     4321 tatgtgcgcg atgagggtta cgctcgtctt cctgagctgg gcaaatgccc tcccacgatc
     4381 cacatcgacc ttcgttcagg cgaggccaga gtgttaaata cctttgggat cggcggtctc
     4441 tttgacattc gaactgcgcc tgcgggagac cggttcgtgc tgcttggaaa ggggatagcg
     4501 gtaatggtcg atgcgatgac cggtcatgtt cttcgtgaag tagcgcatct agccgaatac
     4561 cgccgctctc tttaccagga acctgaggct ggcaaggtct atctcctcgg gtacgaggtc
     4621 gattgggagc aggaagcgct tgattgctgg tggatctcga tggcgcctga aaatgcgggt
     4681 gaagtggaac atgtactggt ttcgatgaag actggcgagc ggctgaaaca gacttcgatg
     4741 agtccctggc cggaactgcc tgactttgac cgtttcgagt ttccgaagac ttttttggga
     4801 tctgtgctat ctaaacgcaa gctggtatct ggaaggaggg aaatgacagg aaacagaccg
     4861 ctgttgcggt tggtagaggc tcacactttg gcacgtctgt ccgctcggcc ggcgcttttg
     4921 gaaaatctcc catcagagaa attggtcacg atttctatat ctcctcaagg acgtttcttg
     4981 ctgggcgagt acttcacgac ggtggaagaa cggacgtggg aagatccccc tgagcattcg
     5041 cgaagaatcg tgattctcga caccgagacc ggcgcaatag aagcggactt cgttttcaag
     5101 aaccccgttg caggagtctt tttcctgggg acacatgaaa acggatgagg actatgctgc
     5161 gtgctacgta accggtcaac aaaatatgga cttggcacat tcaagttcca agtgcacgcc
     5221 atgcgatttt tcgtttctgg aggaagactt cattgagtgt tcggtatccg aggcattttt
     5281 ggtttggttg tttagttcat cgactatctc ctggagtttt tctaatgtag gggcaggccc
     5341 ctgtgcctgc ccatccggat agcctccgcg ctgggtaatc tggatggtta ggagcaggca
     5401 cacgcggcag ccctgttcga gaaaaaattt gagtttcgtg cgttccattg aggtaaggcg
     5461 ggcatagggc gtcgggactc atttcgctct tgggtttcgg ttggcgcctc caagaatacc
     5521 gaatggatcc taatgtcttc ttcactggca actctttccg taaaattcac ctccagtaaa
     5581 agggctaagc ccgaagattg tgcacatcgt atttcgaatg cgcgcctggt gctctcgtcg
     5641 tgtcgccggg ctataatgcc cgggcaatcc catccatgag gagcagccat gccggagata
     5701 cccgcgtacc aaatccagcg gacagcgtcg caacctgaat ttaacggggc ctgggatggg
     5761 ccgctgtggc gctccgtgcc caccgtctct attgcgcact tccatcccgc gagcagcgac
     5821 catcgcccgg tgacgcatgc caaattgctc tacgatgcag acgcgctcta tgccattttc
     5881 cgggtggagg atcagtacgt ccgcgcaacg cgcgaggacc tcaactcatc ggtctgcaac
     5941 gacgcctgcg tcgagttttt cttcgagccc agggctggcg gcggctattt caattttgaa
     6001 atgaactgtc tgggcacgct gcatgccggc tatgtggagg atccaacgcg cacacccgaa
     6061 ggtctgggta aggcgacgag actgctccgc cgccaggcgg cgatgatgcg ggtctatcat
     6121 tcgattccca gcgtggtgtt tcccgagcgt gcggatccgg tgcgttgggt ggtggaatat
     6181 tcaatcccgt ttgcgctgct cgaagaattc accggtccgc tcgccggccc ggccggtcag
     6241 acctggcgcg gaaattttta caaatgcgcg gaagataact cacacccgca ctgggcctcc
     6301 tgggcgccta ttggcgagca gctcaatttc cacgtgccgg agtattttgc gccactgcat
     6361 tttgccggat aagtaagacg gctgcgtctt ttggttctcg cgaggcgcct gaggggcact
     6421 ctgtctccag gcgtgccggc tgggatatac tcgggcgagg gatacaacgc ggtatagcat
     6481 caggagtcac accatgttaa gactggccct tacattagcc atcgccgctg tgttaccgat
     6541 cgcgtctgcc gaggaggctg ccgtgcagct ctacgtcgcg ccgcagggca acgacgcctg
     6601 gtcgggaaaa ttgccggaac ccaacgcgga cggcacggac ggcccactgg ccacgcttac
     6661 ggcagcgcga gaccgattgc gggagatgaa gggtagtcag cggctgcccg ggccggtttc
     6721 cgtgcagctg cgggaaggcc actaccgcat taccgaggcg atcgtctttg aaccgctcga
     6781 cagcggcctg ccaacggcgc cgatcacgta tgaggcctat ccgggtgagc accccgttat
     6841 cagcggaggc gtgccggtga ccggatggac gcaggaaggg ccagcctgga aagcacaggt
     6901 gcccgaggcg ggaccccggc ccgcgccgtt ctgtgcgcta tgggtcaacg gggaatttcg
     6961 aggcccggcg cgcagcccca acgacggaga gttcgtcagc gacgggatcg cgcccgaaat
     7021 caaggacccc gccacggaca agccggtaat cagccagcac aacgccatcc ggtacaaaga
     7081 gggcgacgtt caggaatggc acaacctcga ggacgtgctc gtggtgggcc tgcattcatg
     7141 ggacatcacc atactgccgg tggaccggat cgatcgaggt gagcgcctgc tgatcatgaa
     7201 gcggccggaa ggcggatgga atttcaacca gtggggcggg acgcagcagc ggtattacat
     7261 cgagaacatc ctggaaggcc ttgacgcgcc gggcgagtgg tacctcgacc gaaaggcgaa
     7321 aacggtttta tacctcccca ggccgggtga aaacatgaat gccgtggagg tcatagcgcc
     7381 ggtggcgcgg cagctcatcg tgctgcaggg cgatgcggcg aacggggagt ttgtgtgcca
     7441 tctccatttc aaggggattg cgtttcacca caccgattac agccttcctc cggaaggaat
     7501 gaacagccag caggccgcca gctcggtcca cgcggccatc cagggacgcg ctgcgcggta
     7561 ttgctcgatc gaagattgcg aggttgccca catcagcaat tacgcgatct ggttcggcga
     7621 gggctgccag gacaaccgta tcgttaaaaa ccacatccat gatctgggcg ccggtggcgt
     7681 ccggctgggt gagatgggcg acgcgcccac gaccaaccac gtgtgccgtc tcaacacggt
     7741 ggacaacaac tggattcacg acggcggcac catttaccac ccggcggtcg gcgtgtggct
     7801 gggccgaacc tcgtacaaca ccgtatcgca caatgagatc cacgatttct tctacacggg
     7861 agtatcggtg ggctggtcct ggggctacgc agagagctcg gccaaccaca acatcatcga
     7921 atacaaccat attcatcatc tcggaaaacg ctatctgagt gatatgggcg gcatttatct
     7981 cctgggccgc gcgccgggga ccgagtgccg gtacaaccat atccacgacg tgttctcgtt
     8041 tgactacggt ggttggggga tctaccccga cgaaggcagc tcgtatctgc gcattgagaa
     8101 caatgtcgtg tacaacacca agacgggcgg attccaccag cattacggcc aggaaaaccg
     8161 cgtcgaaaac aacattttcg cctatgcgca cgagggccag attcagcgca cacgtatgga
     8221 agaccacctg tcgttcacgt tccggcgcaa catcgtctat ttcaacaacg gctggctgct
     8281 gagcagcaac tggcgcgaca accaatacga cctcgatttc aactgttatt gggacgaaac
     8341 agtcggctcc gtgctgtttg cggggcattc gctggagcgg tggcgcgaga aaggccagga
     8401 tcggcactcc atcgttgctg atccgctgtt cgagaatccg cacgcgtttg atttccgcct
     8461 gaaaccggaa tcccccgccc tggctctcgg gttcgagccc atcgacatga gcgggatcgg
     8521 cctgtacggc gatgaagcct gggtccaggc gcccggacag atacagcacc cgccctcaga
     8581 actgcctgcg ccgttcaagc caaaatttgt gcacgacgat ttcgagggta cgcccgcggg
     8641 c
//
LOCUS       DAOPBQ010000077         8589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7678_length_8589_cov_3.596672, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000077 DAOPBQ010000000
VERSION     DAOPBQ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7678_length_8589_cov_3.596672"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKW60_03900"
     CDS             <1..201
                     /locus_tag="PKW60_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98605.1"
                     /translation="EEDVLAYAAAWDVPYLDITAEAELTPASFIDTSHIWDMDAAKRY
                     TRVLASHLAALIKNTQLDREEW"
     gene            333..1520
                     /locus_tag="PKW60_03905"
     CDS             333..1520
                     /locus_tag="PKW60_03905"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPJ98606.1"
                     /translation="MIQSGAFWLAVLASAVMFWLLPQRFRTGFLALASMGYLATLAPF
                     SVLALVGWTTAFYYLAPRAAGGDGAKHRLLSILILSILGYLAYFKYIPPLVQAFLPGP
                     TEKQLIIPLGISYFTFKFIHYAIETARGNIEHRSLPDFFCYTFLFPTFTAGPIERFDH
                     FIENRETTWQLQSTVEGLTRIIHGLVKKLVIAEMVLRPLLRDVSPALFLARLDVLPTW
                     KIWIYLAVMFLIYYMDFSAYSDIAIGISRLYGIRIMENFNFPIIAPNISNFWKRWHMT
                     LSGWCQTYVYMPVIGLTRNPYLATVASFLVMGLWHSGSLTRVGWGLYQAAGVAAYLTW
                     TRIKRAKRWRFMERAPWRYASIPITMMFVVPSTAFLIIEYQFPVYEALRILARLVFLH
                     LPA"
     gene            1592..3151
                     /locus_tag="PKW60_03910"
     CDS             1592..3151
                     /locus_tag="PKW60_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPJ98607.1"
                     /translation="MPIYTLYDLLEKNLCDRAGQTALVLGGTQVSYGELANRAEALAA
                     WMHERGIRRGDRVAVHLHKSIEEIIVTFAVVRIGAIIVNVSYQRSVPQLEHILHDCQV
                     RLLFTDRRRAMLAEKAGALRAVQEVVVLGRPDGITNATGWDQLPQCGTAPASRPVDAD
                     LASLHYTSGSTGMPKGVMTTHLNLIDGTHRVADYLHNSAEDRILGLVSLSAPWGLLQV
                     TTMFLRGGTLVLQPVAIPSEIVASINANRVTGLAAFPVSWIEIVAYLQQEPVAMPSLR
                     YITSSGGKIPLPTLEALPRVLPGVDIYLTYGLTEAFRSTVLPPERYQDKMGSLGRPCR
                     NVDVFIVDPAKGICGPGEQGELIHRGTLVTKGYWGDPETTARSFRTCEALRPLIGDEI
                     VHYSGDIVRMDDEGFLWFVERADSVIKCSGYRVSPTEVEDIVQRSGLAHHAVAFGVED
                     DSLGQVVHVAVSAEGRDVDLAGLMQYCRQHMPTFMVPQKFHVWEGAMPIAGTGKVDRR
                     AVIEACQRNEQ"
     gene            3226..3468
                     /locus_tag="PKW60_03915"
     CDS             3226..3468
                     /locus_tag="PKW60_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011747614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPJ98608.1"
                     /translation="MALARNALINYICSTFGLDDGELDDDTLLFSDGLLDSFCMVSLI
                     SYVESEAGITFRPTEVNLDNLDSVARILAFVERKTA"
     gene            3531..4802
                     /locus_tag="PKW60_03920"
     CDS             3531..4802
                     /locus_tag="PKW60_03920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HPJ98609.1"
                     /translation="MEEAQFLSEQQGLGRRRVARAATALLAVYALAVVVKIAVQTDRF
                     QVNFQPYYYAALAFSRGLNPYDSEVTRELAGGEFVLTYAYPPLTLNLLRPFALGSFET
                     SYRCFLALKCVALGLCLWLWQRKFLGSGIDNVFLLVCLIGFNYAIYWDIHSGNISFLA
                     QTVLWLGLYAYLRGRPGWFIALTVGASLFKYTPGFFLVLLLFVEGRKKWGWCAVGGAA
                     FVLLHGASYLLYPQLMPEFLENIGEIQGLVPVEAGPVENFDGAPATWPLIKTLFTNLS
                     EKLSVSSLNALPVFAFAALALAICGITWKVLRADPCADPRECRLVHVVFACLVYLMLT
                     PRLMVGEYTVGLLPAYYIVRRMRYAHASLLLIALMAPSPITGRPPGFKFLVETCTNYY
                     PLLTVYALWALGLYVLWRERSHPPRTQDAVS"
     gene            complement(4958..5809)
                     /locus_tag="PKW60_03925"
     CDS             complement(4958..5809)
                     /locus_tag="PKW60_03925"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HPJ98610.1"
                     /translation="MSLAKKPHLVFTIVLVITVGTGCCSFTPKTTAPHTKVKTHEFHG
                     IEFVWIPPGTFEMGSPDAEPGRGADEGPVHAVTIDKGFWISRREISQKEYALVMNEKP
                     SAVQREEMPVTNVSWLDAMAFCAKLSADSGAAYSLPTEAQWEYAARAGGTGAYHFGND
                     PILLDIYDWYAVNAPNKEVQYGGQRAPNRWGVYDVHGNLSEWCYDVYREDAYTQCCAT
                     ACGAQAEGVARVVRGGSVKSKADECRVAARAQAAPEAKSPTLGFRIVRDKLPTWPKYY
                     RAAGAKN"
     gene            6223..>8589
                     /locus_tag="PKW60_03930"
     CDS             6223..>8589
                     /locus_tag="PKW60_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HPJ98611.1"
                     /translation="MDNITRAAVLGSGVMGGAIAAHLANCGIPSLMLDIIPPGLSADD
                     RRDPAKRNAFAAASKAALLKAKPAPLYLPGNADLIEIGNFEDDFPRISEADWVIEVVK
                     EDLSVKKKVLGEAAKHRRPGSIISTNTSGILIQSMVDEMDDDMRRHFLGAHFFNPPRY
                     LPLLELIPGPDTLPGVVGSLKQFAENVLGKGVVVAKDTPNFVANRILTYACQYIMREF
                     HKDGLSVDDVDSLTGPIIGHASSATFRTCDLVGLDTYVHVIGNVYRGCPDDEQRELFQ
                     PAPWLQKLVDENRLGEKTGAGFYKKTDERDEKGKRVILSLNVNTLEYEPPRKTRFDCI
                     GAARATDNLEEKVRIMHTGEDAGSKFVWKLFAHTAIYAANRIPEIADDIVSIDHAVKW
                     GFAWNIGIFETWDALGFEYVCDRMAADGLAVPALAQALRDAGGTSFYRYENGRKLYFD
                     LASKGYKEVEQNPNALFLLDLKRGHRIVQENAACSLIDLGDGILCAEFHTKMNTIDAD
                     LLELLNAGVARVNEGAFDGMVVANQGDHFSAGANLFMVLGYAMQNDWDALSHIISAFQ
                     DTNMALRFCRGPVVAAPHHYTFGGGIELSQHAARVVAAAETYGGLVEAGVGIIPAGGG
                     TKEMLRRALAYVPDSIPQGDPFPYVRRAFETIGLAKVSTSGAELIPLGYLTEHDVVCV
                     NGQHQVKRAKDVCRGLVLAGYTPPQPAKLTVLGEPVRAAFRSAVYQMQLAGWASEHDA
                     LITEYLARTLTGGDRLPGQVMSEQDVLDLEREAMLYLCGTEKTRARMQH"
BASE COUNT         1567 a         2517 c         2689 g         1816 t
ORIGIN      
        1 gaagaggacg tgttggcgta cgcggcggcg tgggatgtgc cgtacctgga catcaccgct
       61 gaagcggagc tcacgcctgc ctcattcatc gacacgagcc atatctggga tatggacgcc
      121 gcgaaacggt atacgcgcgt gctcgcgtct catttggcgg cgcttataaa gaatacacaa
      181 ctcgacaggg aggaatggtg atggggtttc cccgcctgct cggtattaca ttgcaggccc
      241 tggtactagg cacgctgttg tttcttgccg tcgcgcgact cgtgggcttg gagtcgggtg
      301 cgcgaatctt ccggtatcag gcgttttaag cgatgattca atccggcgcc ttctggctgg
      361 ccgttctggc atcggccgtc atgttctggc tgttaccgca gcgctttcgc acgggcttcc
      421 tggcgctggc gtcgatgggc tacctggcga cgctggcgcc gttcagcgtg ctggccctgg
      481 tgggctggac gacggccttc tactatctgg cgccgcgggc cgcgggcgga gatggggcga
      541 agcaccggct gctgtcgatt ctgatcttgt cgatcctcgg atacctggcg tatttcaaat
      601 acattccgcc tcttgtacag gcgtttttgc cgggtcccac ggaaaaacaa ctgatcattc
      661 cgctggggat cagctatttc acgttcaaat tcattcacta tgcgatcgaa acggcccggg
      721 gcaacattga gcaccggtcc ctgccggatt tcttctgtta taccttcctt ttcccgacgt
      781 ttacggcggg ccccatcgag cgtttcgacc atttcatcga gaaccgcgaa accacctggc
      841 agctgcaatc cacggtggaa ggcctcacgc gcatcattca cggcctggtg aaaaaacttg
      901 tgatcgccga aatggtgctc cggccgctgc tccgggacgt gtcgccggcc ttgttcctgg
      961 cgcggctgga cgtgttgccg acctggaaaa tctggatcta cctcgcggtg atgtttctca
     1021 tctattacat ggatttcagc gcgtattccg acattgccat cgggatcagc cgcctctacg
     1081 gcatccggat catggagaac ttcaatttcc ccattatcgc cccgaacatc agcaatttct
     1141 ggaaacggtg gcacatgacg ctgtcgggct ggtgtcaaac gtatgtgtac atgccggtta
     1201 tcggtctgac gcgcaacccg tacctcgcga cggttgcctc atttctcgtg atggggcttt
     1261 ggcattcggg ctcgctcacg cgcgtgggat ggggtttgta tcaggcggcc ggcgtggccg
     1321 cctacctgac gtggacccgg atcaagcggg cgaaacggtg gcgtttcatg gagcgcgctc
     1381 cgtggcgtta tgccagtatc ccgattacaa tgatgtttgt cgtacccagc acggcgtttc
     1441 tgatcattga ataccagttt cctgtgtatg aagcgcttcg aatactggcg cggctcgtct
     1501 ttttgcatct ccctgcatga agggggatga actgagacgt tccggggccg ggcgcgaaag
     1561 gcgtacccgt accggcggag attgacaggt catgccgatc tataccctct acgatctgct
     1621 cgagaagaac ctctgcgacc gtgccggcca gaccgcgctg gtgctcggcg gcacgcaggt
     1681 cagttatggc gagctggcga accgtgcgga agccctggcg gcctggatgc atgagcgggg
     1741 gatccggcgc ggggatcgcg tggccgtgca cctgcacaag agcatcgaag aaatcatcgt
     1801 gacatttgcc gtggtgcgca tcggcgccat catcgttaac gtgagttacc agcgcagcgt
     1861 gcctcaactg gagcacatcc ttcacgactg ccaggtgaga ctgctgttta ccgaccgccg
     1921 gcgggcgatg ctggcggaaa aggctggcgc gctgcgggcg gtgcaggagg tagtcgtact
     1981 gggccggccc gatggcatta caaatgcaac cggctgggat cagcttccgc agtgcggtac
     2041 ggcgcccgcg agccgtccgg tggacgccga cctggcttcg cttcactaca cctccggttc
     2101 gacgggcatg cccaaagggg ttatgacaac ccacctgaac ctgattgatg gaacccaccg
     2161 cgtagcggac tacctgcaca acagtgctga ggaccgcatc ctcgggctgg tgtcgctgag
     2221 cgcgccgtgg ggcctgcttc aggttacaac catgtttctc cggggcggca cgctggtcct
     2281 gcagcccgtg gcgattccgt cagagattgt ggcgtccatt aacgcgaacc gggtgaccgg
     2341 cctggccgcg tttcccgtca gctggataga gatcgtggcc tatcttcagc aggaaccggt
     2401 cgcgatgccg tcattgcgat acatcaccag ttccggcggc aagattccgc tgccaacgct
     2461 cgaggctctg ccccgggttt tgccgggggt ggacatttat ctgacctatg ggcttacgga
     2521 ggcgtttcgc tcgacggtgc tgcccccgga acgctaccag gacaagatgg gctcgctggg
     2581 gcggccttgc cgcaacgtgg atgtcttcat cgtcgatccc gcaaagggga tatgcggtcc
     2641 cggcgaacag ggggaactga ttcaccgcgg aacactggtc accaaaggct actggggcga
     2701 tccggaaacc acggccagga gcttccggac ctgcgaggcg cttcggccgc tgatcggcga
     2761 cgagattgtc cattacagcg gcgatatcgt gcgcatggac gacgaggggt tcctgtggtt
     2821 cgtcgaacgc gcggattccg tcatcaagtg cagtggctac cgtgtgagcc cgaccgaggt
     2881 ggaggacatt gtgcagcgca gcggcctggc acaccatgcc gtggcctttg gagtggagga
     2941 cgattcactg ggccaggtag tgcacgtcgc tgtctctgcg gagggccgcg acgtggattt
     3001 ggccgggctg atgcagtatt gccgccagca catgcccacg ttcatggttc ctcagaagtt
     3061 ccatgtatgg gagggcgcga tgcccatcgc gggcactgga aaggttgacc ggcgcgctgt
     3121 catcgaggcg tgccagcgca atgagcagta atgaagctcc ggggcgtcgc ggcggagaag
     3181 gtctccgatg gcggcccgga gagaacatcg tggaaggaaa gacgaatggc gcttgcccga
     3241 aacgctctta taaactatat ttgctccacg tttggcctgg acgatggaga attggatgac
     3301 gacaccctgc tgttttccga cggacttctg gactccttct gcatggtgtc gctgatctcg
     3361 tacgtggaat cggaagcggg aatcaccttc cgcccgacgg aggttaatct ggacaatctg
     3421 gattcggtgg cgcggatcct ggcctttgtt gaacgaaaga cggcatgacc ttctgagagg
     3481 tttgaggcgc gccgtgcggc tgacggcgaa ccctgcgagc atcggcagac atggaagagg
     3541 cgcaattttt gagcgaacaa caggggctgg gacggcgacg ggttgcgcgg gcggcgacgg
     3601 ctcttcttgc cgtgtatgcg cttgctgttg tggtcaagat cgcagttcag acggatcgat
     3661 ttcaggtcaa ttttcagccg tattactatg cagcgcttgc cttcagccgg gggctgaacc
     3721 cctatgattc cgaggtcacg cgggagctgg ccggcggtga attcgtgctt acgtacgcct
     3781 atccgccgtt gacgctgaat ctgttgcgtc cgtttgcgct gggcagtttt gagacctcat
     3841 accggtgctt tctggcgctg aaatgcgtgg cgcttgggct gtgcctctgg ctgtggcagc
     3901 gcaaatttct ggggagcggc atcgacaacg tgttcctgct tgtgtgcctg atcgggttca
     3961 attacgcgat ttactgggat attcactcgg gtaacatttc ttttctggcc cagacggtgc
     4021 tgtggctcgg cctgtatgcg tatcttcgcg ggcggccggg gtggttcata gcgcttacgg
     4081 tgggcgcctc gctcttcaaa tatacgccgg ggttttttct tgtcctcctg ctgttcgtag
     4141 agggccggaa gaagtgggga tggtgcgcag tgggaggcgc ggcctttgtc ctccttcacg
     4201 gcgcctcgta tctgctgtat ccccagttga tgcccgagtt tttagagaac atcggcgaga
     4261 ttcaggggct ggtccccgtc gaagccgggc cagtggagaa ttttgacggc gcaccggcca
     4321 cctggcctct gatcaaaacc ctcttcacaa acctgagcga aaagctttca gtctccagcc
     4381 tgaacgcact gcccgtgttc gcgtttgctg cgcttgcgct ggcgatctgc gggattacgt
     4441 ggaaggtcct gcgagctgat ccatgtgcgg acccccggga atgcaggctg gtgcatgtcg
     4501 tatttgcctg cctggtctac ctgatgctga cgccccggct catggtcggc gaatatacgg
     4561 tcggcctgct gccggcgtac tacatcgtcc gccggatgcg ctacgctcac gcgtctctcc
     4621 tgctcattgc cctgatggcg ccgtcaccga ttacgggccg cccccccggc ttcaaattcc
     4681 tcgtcgagac ctgcaccaac tactatccgc tcctgaccgt ttacgcgctc tgggcgctgg
     4741 ggctttatgt cctctggcgg gagcgctcac atccaccgcg cacgcaagac gctgtatcct
     4801 gagcacagcg cggagccggg cagcggggca tataatattg gacgtgtgag accccgtggg
     4861 caggctattg agtgggcgca tcgctgatga aaaggcatgc gcctcccacg cgagcagcgc
     4921 gggaggcgcg ttcaatagca atgacggtga ttcgcggcta attttttgcc ccggcggcgc
     4981 gataatattt gggccacgtg ggcagcttgt cgcgaacgat gcggaatccc agggtcgggc
     5041 ttttcgcttc gggtgcggcc tgggctcgcg cggcgacgcg gcattcgtcc gccttggatt
     5101 tcacggagcc accgcgcaca acgcgggcta cgccttcggc ctgggctccg cacgcagtgg
     5161 cgcagcactg cgtgtacgca tcttcccggt acacgtcata acaccattcg ctgaggttgc
     5221 cgtggacgtc gtagaccccc caccggttcg gggcccgctg tccgccatat tggacttctt
     5281 tgttcggcgc gttcacggca taccagtcgt agatatccag caagatcggg tcatttccga
     5341 aatgatatgc cccggttccg ccggcccgcg cggcatattc ccactgggcc tcggtgggca
     5401 gactgtaggc cgctccactg tcggcggaga gtttcgcgca gaaagccatg gcgtcgagcc
     5461 agctcacgtt ggtgaccggc atctcctcgc gctggactgc cgagggtttc tcgttcatga
     5521 cgagcgcgta ctctttctgg ctgatttcgc ggcggctgat ccagaaacct ttgtcgattg
     5581 tcaccgcatg aaccgggcct tcgtcggctc cccggccggg ctcggcgtcc gggctgccca
     5641 tctcaaaggt tccaggcggg atccacacaa attcgatccc gtggaactca tgagttttca
     5701 ccttcgtgtg cggagccgtg gtcttcggcg taaaagaaca gcatccggtc ccgacggtga
     5761 tgaccaggac gatggtgaaa acgaggtgcg gctttttggc gaggctcata aatctccctc
     5821 ctgacgtgtc gctgatacag cactccacta ccgttccctg ggctccccac acagacgaaa
     5881 agatgcagcg cctttggccc gccctgcaca ctccccatcg ggcggccaac acagtaagag
     5941 tgtaccacgt ctatcattag tatagcatgt gagttgacaa aacgcacgtc ttgctttgtt
     6001 ttcgcagttt cgcttgaccc ctcacgcgtt ctaatgtacg atacatgggt cttggggctg
     6061 aagcagatac aaatggtgcg atctcaacac gttgcaggcc ggatgttcca acggttacca
     6121 tgggctggcc ggcgggcatt tttttgcgaa gccggcacgg cgggcccgga tgtcagaaca
     6181 cgttgacgca gttcggttcg taaggtggaa ggagaaagct ctatggacaa catcacgcgt
     6241 gcggccgtgc tcggctcggg ggtgatggga ggcgcgatcg ctgcacatct cgccaattgt
     6301 ggtattccct ccctgatgct cgacatcatc cctcccggtc tcagcgcgga cgacagacgg
     6361 gatcccgcaa agcgcaatgc gtttgcggcg gcgagcaagg cagcgctgct gaaagccaaa
     6421 ccggccccct tgtacttgcc cggcaatgca gatctcattg aaatcgggaa ttttgaggat
     6481 gatttccccc ggatttccga agctgactgg gtgatcgagg tcgtcaaaga ggatctgtcc
     6541 gtcaagaaga aggtgctggg ggaggcggcg aaacaccgcc ggccgggttc catcatcagc
     6601 acgaacacca gcggcatcct catccaatcc atggtagacg agatggatga cgacatgcgc
     6661 cggcactttc tgggcgccca cttcttcaat ccgccccgat accttcccct gcttgagctc
     6721 atcccgggac cggacacgtt gcccggggtg gtcggctcgc tgaaacagtt cgcggagaat
     6781 gtgctgggca aaggcgtcgt ggtagccaaa gacacgccaa atttcgtggc caaccgcatt
     6841 ttgacgtatg cctgtcaata catcatgcgc gaattccaca aagacggtct ttccgtcgat
     6901 gatgtggaca gcctgaccgg cccgataatt ggccacgcct cttccgccac gttccggacc
     6961 tgcgatctcg tgggtctcga cacctacgtg catgtgatcg gcaatgtgta ccgcggttgc
     7021 cccgacgacg agcagcgcga gctgttccag ccggcgccgt ggctgcagaa gctggtcgac
     7081 gagaaccggc ttggcgaaaa gaccggcgcg gggttctaca aaaagacgga cgagcgcgac
     7141 gaaaaaggca agcgcgtgat tctctctctc aacgtaaata cgctcgaata tgagcctccg
     7201 cgcaagaccc gcttcgactg catcggcgcg gcccgtgcca cggacaacct ggaagaaaag
     7261 gtccggatca tgcacaccgg cgaggatgcc ggctcgaaat tcgtgtggaa gcttttcgca
     7321 cacaccgcca tttatgccgc gaaccgcatc cccgagattg ccgacgatat tgtgagcatc
     7381 gaccacgcgg tgaaatgggg ttttgcctgg aacatcggca tatttgagac gtgggatgcg
     7441 ctcgggttcg agtacgtgtg tgaccgcatg gccgcggatg ggcttgcggt gcccgctctt
     7501 gcacaagcgt tgcgcgacgc cggcggcacc tcattctacc gctacgagaa cggccggaaa
     7561 ctctactttg atcttgcgtc aaagggatac aaggaagtcg aacaaaaccc caacgcgctt
     7621 tttctgcttg acctgaaacg cggccacagg atcgtccagg aaaatgcggc gtgcagcctc
     7681 atcgaccttg gcgacggcat tttgtgcgct gaattccaca ccaagatgaa caccatcgat
     7741 gccgacctgc tcgagctgct caacgcggga gtggcgcggg tcaacgaggg cgcgtttgac
     7801 gggatggttg tggcgaatca gggcgatcat ttctcggcgg gcgccaatct gttcatggtg
     7861 ctggggtacg cgatgcaaaa cgactgggac gccttgagcc acatcatttc ggcgtttcag
     7921 gatacgaaca tggccttgcg cttctgccgc gggccggtgg tggccgcgcc gcaccattac
     7981 accttcggcg gcggcatcga gctcagccag catgccgccc gggtcgtggc cgctgcggaa
     8041 acctatggcg ggctggtcga ggccggtgtg ggcatcatcc ccgcgggcgg cggcacgaaa
     8101 gagatgctcc gccgcgcgct ggcctatgtg ccggacagca ttccccaggg cgacccgttc
     8161 ccgtacgtgc gccgggcctt cgagaccatc ggcctggcca aggtgagcac aagcggcgcc
     8221 gagctgatcc cgctcggtta tttgacggaa cacgatgttg tgtgcgtcaa cggccagcac
     8281 caggtgaaac gcgccaaaga cgtctgccgg ggtctggtgc tcgcgggata cacgccgccc
     8341 caaccggcga aattgaccgt tctgggcgag ccggtccggg cggcgttccg gtcggcggtc
     8401 taccagatgc agctggccgg gtgggcatcc gaacacgatg cgctgattac cgaatacctc
     8461 gcccggacgc tgaccggcgg cgaccgcctg ccggggcagg tcatgagcga gcaggacgtg
     8521 ctcgatctcg aacgcgaggc catgctgtat ttgtgcggca ccgaaaagac ccgcgcgcgc
     8581 atgcaacat
//
LOCUS       DAOPBQ010000078         8527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7735_length_8527_cov_3.480642, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000078 DAOPBQ010000000
VERSION     DAOPBQ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8527
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7735_length_8527_cov_3.480642"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..829
                     /locus_tag="PKW60_03935"
     CDS             5..829
                     /locus_tag="PKW60_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPJ98612.1"
                     /translation="MAEQLTDLSRLCIHTITTKPWPLGECLANYRKAGVPGVTVWRQW
                     LEPQGLDESARMLRDSGLRVTSLCRGGFFPALTEDGRSKALDDNRRAIAEAHAIGAPL
                     IVLVCGAVPGIPLSEARKQIAEGIAAVLPDARAAGVKLSIEPLHPMFSDARSAVNSLT
                     DANDMVEELDDDHVGVTVDVYHVWWDARLEYEIARAGRKILSFHVCDWRTPTRDLLND
                     RGLMGEGCIDIRGIRGWVEQAGFTGDIEVEIFSDAWWATDQNSFVEKIKEAYLAHC"
     gene            complement(948..4328)
                     /gene="ileS"
                     /locus_tag="PKW60_03940"
     CDS             complement(948..4328)
                     /gene="ileS"
                     /locus_tag="PKW60_03940"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPJ98613.1"
                     /translation="MSDHAFEPVPSQFNFSEAEKDVIRFWKENRIYEKSLEARIGAPE
                     FVFYEGPPTANGMPHPGHCLTRTIKDIFPRYKTMTGYYCERKAGWDTHGLPVEIEVCK
                     ELGILDGGKAAIEEYGVERFNRTCVESVFRYQREWEELTDRIGFWVDLEEAYVTYHQS
                     YVESVWWALKQLFDKGLLYQGHKVVWWWAQGGTALSAGEVGEGYRDTDDPAITVRLRL
                     PKASRKALGLDNSNTSLLIWTTTPWTLSSNCAVAVGPDIEYAAVSHTHDGGEEILILA
                     LPLVERYFGEHATVIRIFKASELIGLKYEPLFNYGTPTTVDGSREADAYWQIIPGDFV
                     ELETGTGIVHIAPAFGEDDYRVCQEQGIGFLCYVKPDGTFDERVTDVDPYDQTPFAGQ
                     FCKDADKGITRLLKENGAIIKHEQYRHAYPFCPRADDDPLIQYARRSWFIRTSQYRDE
                     FLANNRLIDWYPEHIRDGRFGNFLENNVDWAISRERYWGTPLPVWVCDKTGYMEAVGS
                     YAELMAKPGAEGVEVWERAKADNPNLVEHLKIHKPYIDAITYASPKDPSARMRRVPEV
                     IDVWFDAGCMPFAQWGYPHLPGSEEQFARRFPADFISEAIDQTRGWFNALLVISTIVH
                     GKDKAPYPHPFKSCICLGHIMGEDGLKLSKRLKNYQEPGLLFDQYSADALRWSFFAKN
                     PPTSTGRLSERNVEEVQRELLIRWYNVYSFFVIYANLDGFNPSTAPAAFLERMDLDPA
                     GATGDGGDAPYAPREDRAELDRWILHELDRTTLDVREAMEDYGCNPAARSIQAFVESL
                     SNWYVRRSREQFWASGWSPEKANAYWTLYECLLKLAQLAAPFVPFFAEITWRNLSKPL
                     PGAPESVHLCAYPSASRDGIDIQLLDEMAAAREAVTLGLSARRTANIKVRQPLSRCEL
                     VLSSDDLRRGLEKHIEMVKSELNVKEIAFTDDPDAYVSFELKPNFKLLGPRLGKKVKS
                     LGKALAQGDTAAMCAQLQNGALTVDLDGETVELTADEVEVRLSPKEGFAAAQGRHMVA
                     VISTEITEELRQEGWVREFIRCVQDIRKDQRLAYDARISLAVTLDDGLLATVLTAYKD
                     KIAGEVLAEAFTIGHGASNEGKQVEIDGVAASVLVNAL"
     gene            4508..4599
                     /locus_tag="PKW60_03945"
     tRNA            4508..4599
                     /locus_tag="PKW60_03945"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4542..4544,aa:Ser,seq:cga)
     gene            complement(4697..6508)
                     /gene="pepF"
                     /locus_tag="PKW60_03950"
     CDS             complement(4697..6508)
                     /gene="pepF"
                     /locus_tag="PKW60_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPJ98614.1"
                     /translation="MAGTKTKSIPPRSAVKKEDTWDLTPLYRSDAAWERAFKKFERMT
                     DGFAVFRGRLHRSAKAIRACFDFQTEFEKLAERLGSYAFLKSAEDVANNTYQAMIARF
                     THMATFANEAASFIAPEIQAIPVDRMRRFQKSKDLEPYRFQLKKLLRYRPHILSHPEE
                     RLLALQGEVAGTAGRIFGQLNDADLKFGFITDEKGVKVELTQSSFRTLLESPKRAVRK
                     QAYEQFYKEYEDHANTLAAALSGSVLQDVYHARVRKFSSCRNEALFSDNVPTSVYDSL
                     IQAVHDNLPTVYRYLELRRKALRVKNLHFYDNYVPIVQSKAWKQTFDEAVGMVMDALA
                     PLGAAYCGPLEKGLRSGRWVDRYENRGKRSGAFSAGGYAGPPYILMNYQADVLDSVFT
                     LAHEAGHSMHTYFSARTQPFQDYDYTIFVAEVASTFNEQLLSAHLLAQASDKKARAFL
                     INREIDEIRGTLVRQTMFAEFEKIIHAIAEAGEPLTLDCLRKEYRALLELYFGPDFEL
                     DESLSLESLRIPHFYSAFYVYKYATGIAAAITLARRVAGGGTRERERYLNFLRAGGSK
                     FPLDLLRDAGVDLTSPAPVATAMERFKTLVAELEELV"
     gene            6728..>8527
                     /locus_tag="PKW60_03955"
     CDS             6728..>8527
                     /locus_tag="PKW60_03955"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HPJ98615.1"
                     /translation="MISILVMLCQLAAADEFAPRLDTLRFHVERLGAITVPEAQQAAK
                     AEKLAELEALLARGAANEDEFTALYLAIDDVRMWLWAHAAEQPAKAEGSFAELDDRWQ
                     IETPGLVLRLMKADFAMEVDAGGHVWRFEPSGKSDIRKGGTEFSLLDAAQRTVEPFAP
                     GYAVGMMITLTEFPDAPGFELRLCANLIGSEIVFELAAVEHERDLAMIRWPKAIIPKQ
                     DPRFVSVIPRMQGMLLPGDWPQAVSAGDLCNSRSFYMPWWGQLDGTAGVMTILETSDD
                     AGGEYAHPEGGPTRIAPRWYPSLGQVRYLRTIRYVFDNDSSYVKMAKRYRRYVQEQGD
                     FVSLAEKRVRTPALDEVIGRPVVHLGALYHFVTEASLFHKDRIEHNHSLQTYDQLAEG
                     LRSLKAAGIDTAYVHLDGWGFYGYDNGHPDVLPAGEEQGGWDGLRRFASTCEELGYFF
                     AVHDQYRDFYFNAVSFDDRLAVVRLDGSREETSTWCGGPQTMLNPRFAPGYVRRNHDL
                     FAAHGINVRGAYLDVFAVVPLEESTLPAHPLTRSDCARYRRDCFDLLRARGYVVSSEE
                     PADYLVRSLDLVHHGPYATYPHIGGGDATGIPVP"
BASE COUNT         1541 a         2498 c         2761 g         1727 t
ORIGIN      
        1 ggccatggcg gaacaactga ccgatttgtc gcggctgtgc attcacacca tcacaacgaa
       61 accgtggccg ctgggcgaat gcctcgccaa ttaccgtaaa gccggggtgc ccggggtgac
      121 ggtatggcgc caatggctgg aaccgcaggg cctggacgaa tcggcgcgca tgctccggga
      181 ttcgggcctg cgtgtgacca gcctctgccg cggcgggttt ttccccgctc taacggaaga
      241 cggacggagc aaagcgctcg acgacaaccg ccgcgccatt gctgaggcgc acgccatcgg
      301 tgcgccgctg attgtgctgg tgtgcggggc cgtaccgggc ataccgcttt cagaagcccg
      361 caagcagatc gctgaaggca ttgccgcagt gttgcccgac gcccgcgcgg cgggggtaaa
      421 actctccatt gaaccgctgc accccatgtt ctccgacgcg cgctcggccg tgaattcact
      481 cacggatgca aacgacatgg ttgaggagct cgacgacgac cacgtgggcg tgacggtgga
      541 cgtgtatcac gtctggtggg acgcccggct cgaatacgaa attgcccgcg cgggccgcaa
      601 gatcctttcg tttcatgtct gcgactggcg cacacccacc cgcgatctcc tcaacgatcg
      661 cgggctgatg ggcgagggtt gtatcgatat ccggggcatc cggggctggg tcgaacaggc
      721 gggcttcacg ggcgacatcg aagtcgagat cttctcggat gcgtggtggg ccaccgatca
      781 gaacagtttc gtggagaaga tcaaagaggc ctacctcgcg cattgttgag gctgcggaac
      841 gagaacccgt tcctgctcga acgtgccgct gaaaacggct cgcgagcccg cacaggcagg
      901 cgcatcgcgc catctccggc ccggcggcat gcccccgtgc acccagccta cagggcgttg
      961 accagcacgg acgcagcgac cccgtcgatc tccacctgct tgccttcgtt ggatgcgccg
     1021 tggccgatcg tgaacgcctc agcgaggact tcaccggcga tcttgtcttt gtatgccgtc
     1081 agcaccgtgg ccagcaggcc gtcgtccagg gtgaccgcca gcgagatgcg ggcgtcgtag
     1141 gcgaggcgct gatccttccg gatatcctga acgcaccgga taaactcgcg cacccagcct
     1201 tcctgacgga gttcttcggt gatctcggtg gaaatgacgg caaccatgtg gcgtccctgc
     1261 gcagcggcga aaccttcttt cggggaaagc cggacctcga cctcgtctgc ggtcagctcg
     1321 acggtctcgc cgtcgagatc gacggtgagt gctccgttct ggagctgtgc gcacatggcg
     1381 gcggtatcgc cctgggcgag ggccttgccg agagatttga ccttctttcc gagacggggc
     1441 ccgagcagct tgaagttggg tttgagctcg aacgacacat aggcgtccgg atcgtcggtg
     1501 aacgcgattt ctttgacgtt caattcgctc ttgaccattt caatatgctt ttcgagtccg
     1561 cggcgcaagt cgtcgctgct gagaacgagt tcgcaacggc tcaggggttg cctgaccttg
     1621 atgttggcgg tgcggcgcgc gctgagcccg agggtaacgg cttcgcgcgc ggcggccatc
     1681 tcatcgagaa gctgtatgtc gatgccgtct ctgctggcgc tgggataggc gcagagatgc
     1741 acgctctcgg gcgcgccggg caagggtttg ctcaggttgc gccacgtgat ctctgcgaag
     1801 aagggcacga acggcgcggc cagctgagcc agtttcagaa ggcattcgta gagagtccaa
     1861 taggcattgg ccttttcagg ggaccatccg ctggcccaga actgctcgcg gctgcggcgc
     1921 acgtaccagt tggagaggct ttccacaaag gcctggatgc tgcgcgcggc cgggttgcag
     1981 ccgtagtctt ccatcgcttc ccggacatca agcgtcgtgc ggtcgagctc atggaggatc
     2041 cagcggtcga gttcggcgcg atcctcgcgt ggtgcatacg gcgcgtctcc tccatcgccg
     2101 gtggcccctg cgggatccag gtccatccgt tcgaggaagg cggccggagc cgtcgagggg
     2161 ttgaaaccat cgagattagc gtagattaca aagaagctgt agacgttgta ccagcggatc
     2221 agcagttcgc gctggacctc ttcgacgttg cgttcagaca gacgccccgt gctggtgggg
     2281 gggttcttgg cgaagaagct ccaccggagg gcgtccgcgc tgtattggtc aaagagcagc
     2341 ccgggctcct ggtaattctt gagacgtttg gagagcttca gcccgtcttc gcccataata
     2401 tgccccagac agatgcagct cttgaagggg tgcgggtagg gcgccttgtc cttcccgtga
     2461 acgatggtgc tgatgaccag aagcgcgttg aaccaccctc gggtctggtc gatggcctcg
     2521 ctgataaagt cggccgggaa ccgccgcgca aactgctctt cgctgccggg aaggtgagga
     2581 tagccccact gggcgaacgg catgcagccc gcgtcaaacc agacgtcgat gacttccggc
     2641 acccggcgca tgcgcgcgga ggggtctttc gggctggcat aggtgatcgc gtcgatgtag
     2701 ggcttgtgga tcttgagatg ctcgaccagg ttgggattgt ccgccttggc gcgctcccag
     2761 acctcaacgc cctctgcgcc gggcttggcc atcagttcgg catacgagcc gacggcttcc
     2821 atgtacccgg ttttgtcaca gacccagacg ggcagcgggg tgccccagta gcgctcgcgc
     2881 gagatggccc agtcgacgtt gttttcgagg aaattcccga aacggccgtc gcggatatgc
     2941 tccggatacc agtcgatgag ccggttgttg gcgagaaatt cgtcgcggta ctgtgaggtc
     3001 cggataaacc agcttcggcg cgcgtactgg atcaggggat cgtcgtcggc acgcggacag
     3061 aacgggtagg cgtgccggta ctgctcgtgc ttgataatcg cgccattttc cttgagcagc
     3121 cgggtgatgc ctttgtcggc gtccttgcag aactgcccgg caaagggagt ctgatcatac
     3181 gggtcgacat cagtgacccg ctcatcgaac gtaccgtcgg gcttgacgta gcacaagaac
     3241 ccgatgccct gttcctggca gacacggtag tcgtcctcac cgaacgcggg cgcgatgtgg
     3301 acgatcccgg tgccggtctc cagttccaca aaatcaccgg ggatgatctg ccagtacgca
     3361 tcggcttcgc ggctgccgtc gaccgtggtg ggcgtgccgt aattgaagag aggctcgtat
     3421 ttgagcccga tgagttccga cgccttgaag atcctgatga cggtggcatg ttcgccgaaa
     3481 taccgttcga ccagaggcaa ggccaggatg aggatctctt ccccgccgtc gtgggtatgg
     3541 ctgacggccg cgtattcgat atccggcccg acagcgacgg cgcagttgct cgagagggtc
     3601 cacggggtcg tcgtccagat caagaggctg gtgttggagt tgtcaaggcc gagcgccttg
     3661 cggctggctt tggggaggcg caaccggacc gtgatggcgg gatcatcggt gtcgcggtag
     3721 ccttcgccga cttcgccggc ggaaagcgcg gtgccgcctt gcgcccacca ccacacgacc
     3781 ttgtgcccct ggtacagaag gcctttgtcg aacagctgct tgagcgccca ccagacgctt
     3841 tcgacgtagc tctggtgata ggtgacgtag gcctcttcga ggtcgaccca gaagccgatg
     3901 cggtcggtga gttcctccca ttcgcgctga taccggaaca ccgactcgac acaggtgcgg
     3961 ttgaaccgct cgacgccgta ttcttcgata gcggccttgc cgccgtcaag gatcccaagc
     4021 tccttacaga cttcaatctc cacaggcagg ccgtgggtgt cccatccggc cttacgctcg
     4081 caataatagc cggtcatggt cttatagcgg ggaaagatgt ccttgatggt gcgcgtaagg
     4141 cagtggccgg gatggggcat gccgtttgcc gtgggcggcc cttcgtagaa gacgaattcc
     4201 ggcgctccga tgcgggcctc caggctcttt tcgtaaatgc ggttttcttt ccagaaccgg
     4261 atcacgtctt tttcggcttc tgagaaattg aactgggacg gcaccggctc gaaagcatgg
     4321 tcactcataa caaaaactat ccttgcgtcg cgtgttcggg tctatagaat tgcgctggca
     4381 ggcgcctcaa ggcggtaatc ccgtgattat ccggattttc aggggagttt ttcaagtaga
     4441 aggcgctcag ggatttttgc tagaatggcg gctcacaaca agttcaatcg tttcaacaca
     4501 gccctgcgga gaggtggccg agtggttgaa ggcggcggcc tcgaaagccg ttgtgctcct
     4561 tttggggtac cgtgggttcg aatcccaccc tctccgccac ttctttttcc acgatcgcgg
     4621 ccattttacg cgcgaggaat gatcctcggc actgcccgcg aaatcctcca gcttcgctgc
     4681 ggtgaaccac ccttcgctag acgagttctt ccagctccgc caccagggtt ttgaagcgtt
     4741 ccatggccgt agcgactggc gcagggctgg tgagatcgac cccggcgtcg cgcaggaggt
     4801 cgagcgggaa tttggacccg ccggctcgca gaaaattcag ataacgctcg cgttctctgg
     4861 taccgcctcc ggcaacccgt ctggccaggg tgatcgcggc agcaatgccc gtggcatatt
     4921 tgtagacgta aaacgcgctg taaaaatgcg gaatccgaag gctttccagc gacaaggact
     4981 cgtccagctc gaaatcgggg ccgaaataga gttcgagcag agcccggtat tccttccgga
     5041 ggcagtcaag cgtgaggggt tcgccggcct cggcgatggc gtgaatgatc ttttcaaatt
     5101 ccgcaaacat ggtctggcgg accagggtgc cccggatttc atcgatctcg cggttgatca
     5161 ggaaggcgcg ggcttttttg tcgctcgcct gcgccagaag gtgcgcgctg agcagttgtt
     5221 cgttaaaagt cgacgccacc tcggcgacga agatggtgta gtcgtaatcc tgaaacggct
     5281 gcgtacgggc gctgaaatag gtgtgcatgg agtggccggc ctcgtgcgcc agcgtgaaaa
     5341 cgctgtcgag cacgtctgcc tggtagttca tgaggatgta ggggggcccc gcgtagccgc
     5401 cggcggaaaa tgcgccgctg cgtttgccgc gattttcata gcgatccacc cagcgccctg
     5461 agcgcaggcc tttctccagc ggcccgcagt aagcggcgcc gaggggcgcc agcgcgtcca
     5521 tcaccatgcc cacggcttcg tcgaatgtct gtttccacgc tttgctctga acgatcggca
     5581 cgtaattgtc gtaaaaatgc aggtttttga cgcggagcgc cttgcggcgc agctcaagat
     5641 agcggtatac cgtcggcaga ttgtcatgca ccgcctggat caggctgtcg tagacagagg
     5701 tgggcacgtt atcggaaaac agcgcctcgt tacggcatga actgaatttg cggacgcggg
     5761 cgtggtacac gtcctggagc acggagccgc tcagcgcagc cgccagggtg ttggcgtgat
     5821 cttcgtactc tttgtagaac tgctcgtagg cctgtttgcg cactgcgcgt ttgggggatt
     5881 caagcagggt gcggaacgag ctctgggtga gctcaacttt gactcccttt tcgtctgtta
     5941 tgaacccgaa tttgaggtcg gcatcgttga gctggccgaa aatgcgcccc gcggtcccgg
     6001 cgacctcgcc ctgcagggcc agaaggcgtt cttcggggtg cgagaggatg tgggggcggt
     6061 agcgcaggag ctttttcaac tggaagcggt agggctcgag atccttggat ttctggaacc
     6121 gccgcatgcg atcgacggga atggcctgaa tctcgggtgc gatgaagctc gcggcctcgt
     6181 tcgcgaaggt ggccatgtga gtgaagcgtg ctatcatcgc ctggtacgtg ttgttggcga
     6241 cgtcttcggc cgatttcaga aacgcgtagg aacccaggcg ctcggcaagc ttctcgaatt
     6301 cggtctgaaa atcgaaacag gcacgaatgg ccttggcgga gcggtggagc cggccgcgaa
     6361 atacggcgaa accgtccgtc atgcgttcga acttcttgaa tgcccgctcc caggcggcat
     6421 cgctccggta aaggggcgtc aagtcccagg tgtcttcttt cttcaccgcg cttctcggtg
     6481 gtatcgattt cgtctttgtt cccgccatgc catcttccct gttatgtcgt gatgcgcttc
     6541 aaactgcagc gcagtataca aaaggagagc gcagtgtccc aaacacgccg cccggagaaa
     6601 ggacgccgtc ctctattgag cgctgcgcgc gtctgggtag aatttgcggc ggcatatgcg
     6661 ccatgatgca gcgatacggc cactgggcat ggcggtccgc ggatttctat caccgggagg
     6721 acttacgatg atctcgattc ttgtcatgtt gtgccagctt gcggcggccg acgagtttgc
     6781 cccgcgcctg gacacgcttc gcttccacgt cgagcgtctc ggcgccatca cggtgcccga
     6841 agcgcagcag gcggcgaagg cggagaaact ggctgaactt gaggccctgc tggccagggg
     6901 cgcagcgaac gaagacgaat tcacggccct gtatctggcc atcgacgacg tccgcatgtg
     6961 gctgtgggct catgcggccg aacaacccgc caaagcggaa ggttcgtttg ctgaattgga
     7021 cgaccggtgg cagatcgaga cgcccggact ggtcttacgc ctgatgaaag ccgattttgc
     7081 gatggaggtg gacgccggcg ggcacgtctg gcggttcgaa ccgtcgggaa aaagcgacat
     7141 ccgcaaaggc gggacggaat tcagcctcct ggacgcggcc cagcggacgg tcgaaccctt
     7201 tgcgccgggc tacgccgtgg gcatgatgat cacgctgacg gaattcccgg atgcgccggg
     7261 gtttgagttg cggctctgcg cgaatctgat cggctcggag atcgttttcg aactggcggc
     7321 ggtcgaacac gaacgcgacc tggccatgat ccggtggccg aaagcgatca ttccgaagca
     7381 ggatccgcgt ttcgtgtcgg tgatcccccg catgcagggg atgcttttgc cgggagactg
     7441 gccgcaggcc gtttcggcgg gcgatctgtg caattcgcgg tcgttctaca tgccctggtg
     7501 ggggcagctt gacggcacgg ccggcgtcat gacgatcctc gagacttccg acgatgccgg
     7561 cggcgaatat gcccaccccg agggcggccc gacgcgcatt gcgccgcgct ggtatcccag
     7621 cctggggcag gtacgttacc tgcgcacgat ccgctacgtg ttcgacaacg acagcagcta
     7681 cgtgaagatg gcgaagcgat accgccgcta cgtgcaggaa cagggagatt tcgtgtcgct
     7741 ggccgagaaa cgggtacgta cccccgccct ggacgaggtc attggccggc cggtggtgca
     7801 tctgggcgcc ctctatcatt ttgtgacgga ggcgtcgctg tttcataagg accgaatcga
     7861 gcacaatcac agcctgcaga cgtacgacca gctggcggaa ggattgcgct cgctgaaggc
     7921 ggcgggtatc gacacggcct atgtccacct cgatggctgg gggttttacg gctacgacaa
     7981 cggccacccc gatgtattgc ccgcgggtga ggaacagggc ggttgggacg gcctgcgccg
     8041 gttcgcctca acgtgtgagg aactcggcta cttttttgcc gtgcatgatc aataccgaga
     8101 cttctatttc aacgcggtat cctttgacga ccgccttgcc gtggtgcgcc ttgacggttc
     8161 gcgcgaagag acgtcgacgt ggtgcggcgg cccacagacg atgctgaatc cacgcttcgc
     8221 gcccggctac gtccggcgga accacgattt attcgcggca cacgggatca acgtgcgcgg
     8281 cgcgtatctc gacgtgtttg cggttgtgcc cctcgaggag agcacgctgc cggcccatcc
     8341 gcttacgcgc agcgattgcg cccggtaccg ccgcgactgc tttgacctgc tccgcgcgcg
     8401 gggctatgtc gtgagttcgg aggagcccgc cgactacctc gtgcgttcgc tcgacctggt
     8461 ccatcacggc ccgtatgcga cctatcctca tatcggcgga ggcgatgcca cgggcatacc
     8521 cgtgccg
//
LOCUS       DAOPBQ010000079         8511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7752_length_8511_cov_4.201750, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000079 DAOPBQ010000000
VERSION     DAOPBQ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7752_length_8511_cov_4.201750"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PKW60_03960"
     CDS             complement(<1..967)
                     /locus_tag="PKW60_03960"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPJ98616.1"
                     /translation="MAPHDVSMRNKPSQPRAQVLVASGDIPYANTLASFLEGEGYQVE
                     RCHDVPGVLRLVGRNAFDIVVLDLELDEASDLDLVTFIRRQTPGAQLILLVEIARIEK
                     ALAGIRRGAFFYLPKNVAPSDVALAVNKARRNLQTQSAAHDYEQTLFQEFIGDSQAMQ
                     RVIEVITKVAPTDSTVLILGESGTGKEVIANAIHRLSRRRDMPFIAVNCAALPEQLLE
                     SEMFGHLKGAFTGADGNKRGLFEEADGGTIFLDEVGEMSLATQAKLLRVLQNGEIRPV
                     GSSTSSHVDVRVLAATNRNLEEAVAERRFREDLYFRLNVIQVRIPP"
     gene            complement(1112..1459)
                     /locus_tag="PKW60_03965"
     CDS             complement(1112..1459)
                     /locus_tag="PKW60_03965"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPJ98617.1"
                     /translation="MERRRHIRHFVETGAWLCPPHERQPVPVRSADLSVSGVRLHWVQ
                     PVAPDTPLLVRLQLSEAGPVLECKGRVCWCAPLSNDLYQFGVRFLDVTDDELEQVEGH
                     LRSTRARPVLAAV"
     gene            complement(1745..2767)
                     /gene="ruvB"
                     /locus_tag="PKW60_03970"
     CDS             complement(1745..2767)
                     /gene="ruvB"
                     /locus_tag="PKW60_03970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPJ98618.1"
                     /translation="MHRDEVLDPSPHDGESELDEQIRPHRLDDFPGQEPIKERLRISI
                     EAVKARGEPLDHILMCGPPGLGKTTLARILSHEMGVDIKSTAGPVVERQADLSAILTS
                     LEEFDILFIDEIHRLNHAVEETLYSAMEDFEVDIMLGKGPTARSLKIGLKPFTLIGAT
                     TRAGLLTPPLRARFGDMCRFDFYSPEELKIILMRSARILSTPIDDSGAYEIARRSRGT
                     ARIANRLLRRVREYAEVKAGGAITREVADAALQLHRIDELGLDDMDKTIIATVIDKFS
                     GGPVGVNSLAVAIGEEPQTIEEVHEPYLIQLGFIKRTSQGRVALPAAYRHFGLTAPEG
                     PQGALF"
     gene            complement(2811..3422)
                     /gene="ruvA"
                     /locus_tag="PKW60_03975"
     CDS             complement(2811..3422)
                     /gene="ruvA"
                     /locus_tag="PKW60_03975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPJ98619.1"
                     /translation="MFAFLKGVVAQKSLNRVVLDVGGVGFDVQVPADVHRRLTVHAET
                     TLLTYCHIREDSFQIFGFLREEEKALFELLLGITGIGPKMALALMSALSVQQFGQAVH
                     ENNVAALTKAPGVGKKTAQRIVLEVKTRLGQDTELSALLGETESDVYPQEDDVLEALT
                     ALGCTIGEARRAAAQARNQLGAEAPPEELVRAALQSLARVKLK"
     gene            complement(3552..4595)
                     /locus_tag="PKW60_03980"
     CDS             complement(3552..4595)
                     /locus_tag="PKW60_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HPJ98620.1"
                     /translation="MKTPRRVINAMAPIRICDLGGWTDTWFAETGAVFNIGVYPYAEV
                     QIYVREMPSDAPRIVIHAENYDMRYAIDPHKIAYDRHPLLEASIDIMDLPDHLSFEIN
                     LYSRAPGGCSTGTSAAITTALLGALDLLTPGRRTPHEIARLAHRVETEKLGLQCGIQD
                     QICSAHGGICFIEMYQYPLASVSNIQVSNDIWWELERRLVLVYLGRTHSSSEVHGHVI
                     QRLETENADKSVLEPLRQAAVAGKNAVYAGNFDALGAAMIRNTEAQAALHPKLVSQDA
                     RAVIEAARHYGAVGWKVNGAGGDGGSLTILCDAEGEHKRKFIEAVPGIDPRFQVIPTY
                     LSRVGLRRWECYE"
     gene            4841..6673
                     /locus_tag="PKW60_03985"
     CDS             4841..6673
                     /locus_tag="PKW60_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPJ98621.1"
                     /translation="MESESRTRKRRSTHQVVTVLLVFLMGVALGWVLRGSPEQPGGAT
                     LPVELTAGPEEPPVAETPPVEDAPPPETWIPEVAQVLDAAGDSDEIPISQPPEDEPPA
                     EAPVEEMPPASPPMVLEDVWPARHLFIAISGGTLNNATKALLSDLKPGGVVLLDGNIE
                     NRNQTMKLVSSIKEAVGLGKELYDLPLIAVEQEGGIFNRLNLESAPGAPELGARRDPQ
                     AANAVGLSFGKACRDRGIAVLLAPVLDVVLPGAPDVIKARSFGKDRDVVQALGLAFAD
                     GAMQAGVFPVVKHYPGHGAAKLEPQTTLAVIDLNESHELAKVLYPFAQAVVAGVPGIL
                     VGHIAVPLLDTLEPQRPASLSPVLTRGILRDRWTFKGVILSDDIALAASATGGDAGKA
                     AVLALAAGCDALILADPSPERIRGICDTIEAAVNDGTLSRENLNESKRRLEAMQAWLK
                     EPKGLPGGVPKLAPESSTPVEVAAEAAPEPAKPAKAAPEPPKPAEAAPEAPKPAEPAP
                     KPAEPKPETPKPAEPAPKPAEPKPETPKPAEPAPQAPKPAETTPVKAASSGVSATYHE
                     VQPGDTLTRLSVRYGVSIKELKEWNNLDGDTIQLGKKLRVSPSR"
     gene            complement(6726..7583)
                     /locus_tag="PKW60_03990"
     CDS             complement(6726..7583)
                     /locus_tag="PKW60_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98622.1"
                     /translation="MKSAITGLVALLVGLGAGFWFGMGRVNALQSELEKVRSDGSDLL
                     GRLQALQSDLAERETELGRLREEFNLARSQPAAEIPASTTVPEVEDFDSAELFAQLAQ
                     ALDEAGGPGQRQGRFGDRSGDRFRGTRRPPEEGREQREDRRRSYADDYRTRMSDFLSN
                     EIEASTDPAEKARLASISEYMQSLMEQRQLLRDAQSDEERQAIFETMRQNGDTLRSMV
                     EEQQDQVLRDALARQGVSGRAQQDGIIGAYKEAQRGPFFSGPFTFFGGGWRGRGGPPL
                     MAPPGGEPR"
     gene            7794..>8511
                     /locus_tag="PKW60_03995"
     CDS             7794..>8511
                     /locus_tag="PKW60_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPJ98623.1"
                     /translation="MTALDAALTHVRSHHEESLEQLKTLVAIPSVSTLPEHGQDMIRA
                     AKWLRGALLDVGMSRAELIPTPLHPMVYAEWLGAPGKPTVLVYGHYDVQPADPLDEWN
                     SPPFEPTLRGDCLYARGVSDMKGQIFAQIKAVEALALQGDLPVNLKYLIEGEEEIGSP
                     NLDAFIDANRDLLACDFVLNCDSGILAPELPSLVYSLRGLAYFELEVRGPAKDLHSGL
                     FGGSIHNPAQALCELVAGMHD"
BASE COUNT         1610 a         2610 c         2706 g         1585 t
ORIGIN      
        1 gcggcgggat gcgcacctgg atgacattga gacggaaata aaggtcttcg cgaaaacggc
       61 gttccgcgac cgcctcttcc agattgcggt tggtggccgc cagcacgcgc acatcgacgt
      121 gcgatgacgt cgaggagccg acgggccgga tctcgccgtt ctggagcacc cggagcagct
      181 tggcctgggt ggcgaggctc atttccccga cttcgtcaag aaagatggtg ccgccgtcgg
      241 cttcttcaaa aagaccccgc ttgttgccat cggcgccggt gaatgccccc ttgagatggc
      301 cgaacatctc gctttcgagg agttgttcgg gcagcgccgc gcagttgacc gcaatgaaag
      361 gcatgtcccg ccgccggctc agccggtgaa tggcattggc gatgacttcc ttgccggtgc
      421 cgctctcgcc gaggatcagg accgtgctgt ccgtaggcgc gaccttggta atgacctcga
      481 tcacgcgctg cattgcctgg ctgtcgccga taaattcctg aaacagggtc tgttcgtaat
      541 cgtgcgcggc gctttgtgtc tggaggtttc gccgcgcctt atttacagcc agggccacgt
      601 cggagggtgc cacgtttttc gggagataga agaaagcgcc ccgccggatg cccgcaagtg
      661 ccttttcaat gcgcgcaatc tcgaccagaa ggatcagttg tgcgccgggg gtctgccgcc
      721 ggatgaacgt gaccagatcc aggtcgcttg cctcgtccag ttccagatcc aggacgacga
      781 tatcgaaggc gttccggccc accaggcgca gcacccccgg gacatcatgg cagcgctcaa
      841 cctggtatcc ttcgccttcg agaaagctcg cgagcgtatt ggcgtacggt atatctccgc
      901 tcgccacaag cacttgtgcc cgtggttgcg aaggcttgtt gcgcatcgaa acatcgtgtg
      961 gtgccatgaa tactattgta ccaaaacgaa gcgatgctga caatcaccgc tcaagttgcg
     1021 gcgcagcttg agcaaccgcg gagagataag aggcggccag gcccgcggga tcggattcgg
     1081 ggagttggca aatgcggtgt cgtacgggag gttacacagc tgccaatacg ggcctggccc
     1141 tggtagaacg gagatgcccc tccacttgtt cgagttcatc atcagtcacg tcgaggaaac
     1201 ggacgccaaa ctggtacaaa tcgtttgaga gcggggcgca ccagcacacg cggcccttgc
     1261 attcgaggac ggggccggcc tcgctcagct gcagtcggac cagcaacggg gtgtccggag
     1321 cgacaggctg cacccagtga agccgcacgc cggacacgga cagatcggcg gagcgaacgg
     1381 ggacaggctg ccgctcgtgc ggcgggcaaa gccaggcgcc cgtctcaacg aaatgccgta
     1441 tgtgccgtct gcgttccatt tgtaaaccat gtccttgtat ttgcgtggta tcggatgcgc
     1501 caattgctgc cgttcagtgc ggtttcatgc ccgcggagta ttctcgtgtt tactgacgat
     1561 gcctcgcgcc attcattcaa ttacgctcaa gccgtttgtc gaccgctgga cacgcgcttt
     1621 ccggctcgaa cgcagaaagc atccttctaa aactattgat caccatgccg cgtaaatagt
     1681 tccgttagat tggcaaacac ataaacgggg tggaaagtgc gggatttgcg gagaaggcgg
     1741 cgggttagaa cagcgcgccc tgcggtcctt ccggggccgt gaggccaaaa tgccggtagg
     1801 ccgcgggcag agccacgcgc ccctgtgagg tgcgcttgat gaatccgagc tggataagat
     1861 agggctcgtg gacttcctca atggtctggg gctcttcgcc gatggcgacc gcgagggaat
     1921 tgacgcccac aggaccgccg ctgaacttgt cgatcacggt ggcgatgatg gtcttgtcca
     1981 tatcatccag gccgagctcg tcgatgcggt gcagctgcag ggcggcatcg gcgacctcgc
     2041 gggtgatggc accgccggct ttgacttcgg cgtattcacg cacgcggcgc agcaggcggt
     2101 tggcgattcg ggccgtaccg cgcgaccggc gcgcgatctc gtacgcgccg ctgtcgtcga
     2161 tgggggtcga gaggatccgc gcggagcgca tgagaatgat cttcaattcc tcgggagaat
     2221 agaaatcgaa gcggcacatg tcgccgaacc gcgcacgaag cggtggggtc agcagcccgg
     2281 cccgggtcgt cgcgccgatg agggtgaacg gtttcagtcc gatcttgagt gaacgggcgg
     2341 tgggcccttt gcccagcatg atgtcgacct cgaaatcctc catggccgaa tagagcgtct
     2401 cttcgacggc atgattgagc cggtggatct cgtcgatgaa caggatgtcg aactcttcca
     2461 ggctggtcag gatggccgaa agatcggcct ggcgctcgac gaccggcccc gcggtggatt
     2521 tgatgtcgac gcccatctcg tgtgagagaa tgcgtgcaag cgtcgtcttg cccagcccgg
     2581 gcggcccgca catgaggatg tggtcgagcg gttctccgcg ggccttcacg gcttcgatgg
     2641 agatgcgcaa ccgttccttg atcggctcct gtcccggaaa gtcgtccagg cggtgcgggc
     2701 ggatctgttc atccagttcg ctctcgccgt catgagggct cgggtccagg acttcgtctc
     2761 tatgcatcat atccgtaggc cgttggcgtg ccgtgcgcag cgcggctcac tcatttcagt
     2821 tttacccggg cgagcgactg gagcgccgcg cggaccagct cttcgggggg cgcttcggcc
     2881 ccgagttggt ttcgcgcctg tgcagcggcc cggcgcgcct cgccgatggt gcagcccagc
     2941 gcggtcaacg cttcgagcac atcgtcttcc tgcgggtata catcggactc ggtctcgccg
     3001 agcagagcgc tcagctcggt atcctgtccc agccgggttt tcacctccag cacaatgcgc
     3061 tgggccgtct tcttgcctac gcccggcgct tttgtcagcg ccgccacgtt gttttcgtgc
     3121 acggcctggc caaactgctg aacggacagg gccgacatca aagcgagggc cattttgggg
     3181 ccgatccccg tgatgcccag caacaattcg aacaatgcct tttcttcttc gcggagaaaa
     3241 ccgaagatct gaaaactatc ctcgcggatg tgacagtacg tcagcagcgt cgtttccgca
     3301 tgcacggtca ggcgccggtg cacgtccgcc ggcacctgca cgtcaaaccc cacgccgccc
     3361 acatccagca cgacgcggtt gaggctcttc tgtgcgacca cccctttgag gaaagcaaac
     3421 atagcgccat ggtatccgcg catctccccg cgatcaacct gggccgcgcg ggtacaccct
     3481 ccattctcct caggcgaaaa gaaaggcgcg agggatacgg cctcggaata ggccagtgcc
     3541 ccccaaaaat cctactcgta gcactcccag cggcgcaggc cgacccggga aaggtaggtc
     3601 gggatcacct ggaatcgcgg gtcaattccg ggcacggcct cgatgaactt gcgcttatgt
     3661 tcgccttccg cgtcgcacag gatggtgagc gacccgccgt cgccgccggc cccgttgact
     3721 ttccagccca cggcaccata gtggcgcgcc gcttcgatga ccgcccgggc gtcctgcgac
     3781 acgagcttgg ggtgcagtgc tgcctgggcc tcggtattac ggatcatcgc ggcgcccagc
     3841 gcatcaaagt tgcccgcata caccgcgttt ttgccggcga ccgcggcctg ccgcaagggc
     3901 tcgagaaccg atttatcggc gttttcggtt tccaggcgct ggatgacgtg gccgtgcacc
     3961 tcgctggacg aatgcgttcg gccgagatac acgaggacga ggcgccgctc caattcccac
     4021 cagatgtcgt tggaaacctg gatattggag acgctggcca gcggatattg atacatctcg
     4081 atgaagcaga tcccgccgtg ggcggagcaa atttgatcct ggataccgca ctgaaggccg
     4141 agtttttccg tttcgacgcg atgggcgagc cgggcgattt catgcggggt ccggcggccc
     4201 ggcgtgagaa ggtccagggc gccgagcaga gcggtggtga tggcggcgga cgtgccggtg
     4261 gaacaaccgc cgggcgcgcg cgaatacaga ttgatctcaa aactgaggtg gtcgggcaga
     4321 tccatgatgt cgatggatgc ctcaagcagg ggatggcgat cgtaggcaat cttgtgcggg
     4381 tcgatggcat agcgcatgtc gtagttttcg gcatggatga cgatgcgcgg ggcatcggac
     4441 ggcatctcac gcacatagat ctggacctcg gcgtacggat acacaccgat gttgaatacc
     4501 gcgccggtct cggcgaacca ggtatcggtc cagcccccca gatcacagat gcggatgggg
     4561 gccatcgcgt tgatgacacg tcgtggagtt ttcatgaagt ccattcctgc cgtgaaggtt
     4621 ttcaggttcc ccggagaagc ttaccgcatc ggggcatacc ggacaaacag cgcgccggcc
     4681 cggcaatctg acccgaatca atggacgcgg cgccggattc ggggtattat acagcgcgta
     4741 gccctacggg gagttcaacg ggctgcagag gccgcggccc gcgggcgcat tctgccccgg
     4801 cggccaaacc aaccacgtgc cgaacaggct ggagggaacc gtggaatctg aaagcagaac
     4861 gcgcaagcgc cgctccactc atcaagtggt caccgtgttg ctggtgtttc tcatgggcgt
     4921 ggcgctcggc tgggtgcttc ggggcagtcc cgaacagccg ggcggcgcaa ccctgcctgt
     4981 cgaattaacg gctgggcccg aagagccgcc ggttgcggaa acgccgccgg tcgaggatgc
     5041 gccgccaccc gaaacctgga ttccggaggt ggcgcaggtg ctggatgcgg cgggcgactc
     5101 ggacgaaatt cccatttcac agcccccgga agacgagccg ccggcggaag cgcccgtgga
     5161 ggaaatgccg cctgcttctc cgccgatggt ccttgaagat gtgtggcctg cccggcatct
     5221 cttcatcgcc atcagcggag gaaccctcaa caacgccacc aaagcgctgt tatcggatct
     5281 taagccgggc ggcgtggtat tgcttgacgg caacatcgaa aaccgtaacc agacgatgaa
     5341 gctggtgtcg agcattaaag aggccgtagg actgggtaaa gaactctacg atttgcccct
     5401 gatcgccgtc gagcaggagg gcggcatatt caaccggctg aacctggaat ctgcgccggg
     5461 cgcgcccgag cttggcgccc ggcgtgaccc ccaggcggca aatgcggtgg gtctgagttt
     5521 cggtaaggcc tgcagagatc ggggcatcgc ggtgctgctg gcgcccgtgc tcgacgtggt
     5581 gctgcccggc gcgccggacg tgatcaaggc gcgcagtttc ggcaaggacc gggacgtggt
     5641 gcaggccctc ggcctcgcat ttgccgacgg tgcgatgcag gctggtgtgt ttcctgtcgt
     5701 aaagcactat cccggccacg gggccgcgaa actcgaaccg cagaccacgc tggcggtgat
     5761 cgacttgaac gagagccacg aactggcaaa agtgctgtac cccttcgctc aggccgtggt
     5821 ggcgggtgtg ccggggatac tcgtggggca tatcgcggta ccgctccttg acacgcttga
     5881 gccgcagcgc ccggcatctc tgtcgccggt attgacacgc gggatcttgc gtgaccggtg
     5941 gacgttcaaa ggcgtgattc taagtgacga catcgcgctg gccgcgtccg ctaccggcgg
     6001 agatgcaggg aaagccgcgg tactggccct ggccgcggga tgtgatgcgc tgattctcgc
     6061 ggacccttcg ccggagcgca tccgtggaat atgcgatacc attgaggcgg cggtcaatga
     6121 cggaacgctg tcgcgggaaa atctcaatga aagtaagcgc cgtctggaag ccatgcaggc
     6181 ctggttgaag gagcccaagg ggttgccggg cggcgtgcct aagctcgccc cggagagctc
     6241 gactccggtc gaagtggcgg ccgaagctgc gcccgaaccc gcaaagcccg ccaaggctgc
     6301 cccagagccg ccgaaaccgg ccgaagcagc gccggaagcg cccaaaccgg ccgagcctgc
     6361 gccaaaaccg gccgaaccaa agccggaaac gcccaaaccg gctgagcccg caccaaaacc
     6421 ggccgaacca aagccagaaa cgcctaaacc ggccgaacct gcgcctcaag cgccgaaacc
     6481 cgccgaaacg acgcctgtta aggctgcgtc gagtggggtt tccgccacgt accacgaggt
     6541 ccagccgggc gacacgttga cccggctttc agtgcggtac ggcgtctcca tcaaagaact
     6601 gaaggaatgg aacaatctgg acggggacac gatccagctc gggaagaaac tgcgcgtcag
     6661 cccgtcccga tgagcgcacg gcgggcgtgc cctctaatgc cctgttgaag aagcaggcgg
     6721 gatcctcacc gcggttctcc gccgggaggc gccatcaggg gcggtcctcc gcgcccgcgc
     6781 cagccgcccc caaaaaacgt gaacgggccg ctgaaaaaag ggccgcgctg tgcttcttta
     6841 taggcgccga tgatgccatc ctgctgtgct ctgcctgaga cgccctggcg tgcaagggcg
     6901 tcgcgcagga cctgatcctg ctgttcctcg accatggacc gcaaggtatc accgttttgc
     6961 cgcatggtct cgaagatggc ctgacgttcc tcgtcggact gggcatcgcg caggagctgg
     7021 cgctgctcca tgaggctctg catatactcg ctgatggacg cgagccgtgc tttctcagcg
     7081 ggatcggtgc tggcctcgat ctcgttcgag agaaaatcgc tcatacgtgt gcggtaatca
     7141 tccgcatagc tgcgccggcg gtcttcgcgc tgttcacggc cctcttcggg aggccgccgc
     7201 gtgcctctga accggtcgcc agatcggtcg ccaaaccggc cctgccgctg cccgggcccg
     7261 ccagcctcat cgagtgcctg ggccagttgc gcgaacaatt cggcgctgtc gaagtcttcc
     7321 acttccggta ctgttgtgct cgccgggatt tcggcagcgg gctgggaacg cgccaggttg
     7381 aattcttcgc gtaaacggcc aagctcggtc tcgcgttcag ccaggtcgct ctgcagagcc
     7441 tgaagcctgc ccagcagatc cgagccatcg ctccggactt tttccagttc gctctggagg
     7501 gcgtttacgc gccccattcc gaaccagaaa cccgcaccca atcccacgag cagagccacc
     7561 aggcccgtta ttgctgattt cattgtgcgc ctccttcgaa gtgacgagtt cgaagatttc
     7621 tcattatcgt tgcatggagt cgaaagagag tcaagcatgg atcacacaaa gaaagggcgc
     7681 catttccggc cggccgacac ttagcattag aatggacggc gtggcagtga attcaaaagc
     7741 ctgtggtatg atcatacgcc gtgcaagcaa cccaagccgc cgaaggagga caaatgaccg
     7801 cattggacgc cgcattgact cacgtgcgct cccaccacga ggaatccctc gaacaattga
     7861 aaactctggt ggccatcccc agcgtctcga ccttgccgga gcacgggcag gacatgatcc
     7921 gcgcggccaa atggctgcgc ggcgcgctcc ttgacgtggg catgtcgcgc gcggaactga
     7981 tacccacccc gctccatccg atggtttatg cggaatggct gggggcgccg ggcaaaccca
     8041 ccgtgctggt gtatggccat tacgatgttc agccggccga cccgcttgac gaatggaatt
     8101 cgccgccgtt cgagcccacg cttcgaggag actgcctgta tgcccgcggc gtgtcggaca
     8161 tgaaaggcca gattttcgcg cagatcaagg ccgtcgaggc gctcgccctc cagggcgatc
     8221 tcccggtcaa cctcaaatac ctgatcgaag gcgaagaaga gatcggctcg ccgaatcttg
     8281 atgcgttcat cgatgcgaac cgcgatttgc tcgcctgcga ttttgtgttg aactgcgatt
     8341 cgggcatcct cgccccggaa ctgccgtcgc tggtgtattc gctgcgcggt ctggcctatt
     8401 tcgagctgga ggtccgcgga ccggccaaag atctgcattc cggtttgttt ggcggctcga
     8461 tccacaatcc agcgcaggcc ttgtgcgaac tcgtggccgg catgcacgac g
//
LOCUS       DAOPBQ010000080         8496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7764_length_8496_cov_3.253406, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000080 DAOPBQ010000000
VERSION     DAOPBQ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7764_length_8496_cov_3.253406"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PKW60_04000"
     CDS             <1..1125
                     /locus_tag="PKW60_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98624.1"
                     /translation="ALTALAVYMLTVRSGSFRWLLLAVPLTVLATLAAPENAGLALVC
                     ALFETVCASDSARKRIMRLAPVLLAVLGASLTHGKAMFAHDGNVAAMFGPLHLILYPI
                     GFLPITARYIQEIPVLGWLMAVPVVTLIALIYWKARRPMILFGLLAMPAVRIFGGGRP
                     FEPVHLIGGGQLLLANAFFAIALTALFHRIMDHPKWRRLTVSGTSLLCLIFMVVQMHA
                     LVQWRSAGNLLRDFHTQLRMMKNETLEPVFEEALLISPDIHYVEGAPVCLSASLMIGD
                     QNVPYRPDVPTFSGGVFGGPKINIFRRKGVYVQSSIEDDRVIAVVEGATHFDVVPLED
                     CWKWNVERGGRDPIIWAELPEGNPHLLVFPGQFPWPSPEP"
     gene            1525..1668
                     /locus_tag="PKW60_04005"
     CDS             1525..1668
                     /locus_tag="PKW60_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98625.1"
                     /translation="MPALHKITFTVAVTALTTLLVILCYIVFSYIWSSLAAIREAGRA
                     LWP"
     gene            complement(1693..2475)
                     /locus_tag="PKW60_04010"
     CDS             complement(1693..2475)
                     /locus_tag="PKW60_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPJ98626.1"
                     /translation="MTDKISRRTAIKSAAVLTVAVAAGESAQSQTAGNPAPAVADELA
                     RVAGFLEGNQAIKWVFAGDSITHGALHTYGWRDYPQLFEERVRYELGQERNLVIRTAT
                     SGWTLGRLAEDLDWRVLQFAPQVASFHFGMNDCQAGPEALPRFREQYLDVVERVRAGC
                     GAAIVIHTPNPVLPCTEAQRASNLESYVVVMREVAEQSGAILVDHFAAFNEYARKSNL
                     HYLMNDLIHPNEFGHRFKANLLFNALGIFAERSNVCQLSIPL"
     gene            complement(2518..4596)
                     /locus_tag="PKW60_04015"
     CDS             complement(2518..4596)
                     /locus_tag="PKW60_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPJ98627.1"
                     /translation="MSAVYVLTLGLSSAVAGAFVHSLLARLGFAPTFELSTRLAFGGA
                     AAYITVQLAWVALVKALKPTRARKFLLSECLSHLAAVVLIPYLLHIPIRWPDPALAKA
                     EPLLFLGGYAALHAALKLFSFYTALYSEPSRRLPVLAWLGAAGVAAGATIIALHQWHA
                     GIEAARPEAASMPGIYRAGGAYAEARPIPEGAMARYDLPIYENRGITLRWANLPDATP
                     DEAVRRAYVTVVIEGEEKDKYEAAVSLSESAWATLRVPADAIPAGATRCAVFWTQSPS
                     PTWMRLLGFLPVVHSSRQLLMSGPLLHRTHAEKTPPNLVLIGIDGLSSDRMSAWGYRR
                     RTTPQLDRLVHSSVAFRYGYSPAPDASAAYMTCFTGVNPLCHGYFGGAQGPLPEKART
                     LAALLRENHYATAAFTEGEYRGDLEFGSGFENGFECFDAKYAEDEEGSQRTVEQACAW
                     MDEHQDVAFMLFVRAGDLADAKSRERLGARPSNQPGPAKDTELYDAALESMDALIGGL
                     IQHIRDSAYRDYTSILLFSPYGVSFGADGGQLAPELTEDDMRVPILYYAPEMVREKRE
                     YLVGLEDIAPALSRVAGLSREAALEGRSFLFGPVGKDPVSMMAKPFRLSVRSGNWRYV
                     WEPGPSAFGELPAPGTGESALYRVGASTRRATIASEQKALVAELQKRLTDYVQSSYQW
                     RGVAASAPAK"
     gene            4723..7134
                     /gene="secA"
                     /locus_tag="PKW60_04020"
                     /pseudo
     CDS             4723..7134
                     /gene="secA"
                     /locus_tag="PKW60_04020"
                     /inference="COORDINATES: protein motif:HMM:NF006630.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
     gene            7369..7476
                     /locus_tag="PKW60_04025"
                     /pseudo
     CDS             7369..7476
                     /locus_tag="PKW60_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
     gene            7449..>8496
                     /locus_tag="PKW60_04030"
     CDS             7449..>8496
                     /locus_tag="PKW60_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98628.1"
                     /translation="MLRRHRKKLIGAAIVLFWLLMMGLLGRREILPLLRGPSLSTNYP
                     TSPTDSWMMVLSPGEQPLGFINTQTAPETRDGLPGTLLTLNAILKMQFLGQPMRMHLG
                     GSAWFARTGDAGRFDFAVRSEEHSARFEGVLGNGVLNARIHTAGEVYPLSLPVNRELA
                     IAGIAGTSALQLDRLKPGEEAVIEAFDPMTLSMGTARVKCLGFDTLGEGDEARKVRVV
                     SITSHNIESKAWLDEEGQTLRAETPLGLILQQTSSSEALSAVSREVPQDLIQAVAIHP
                     SGKRPVRGANLMRIRISGIPDAVTIPEDANQRRTADGDLLIAPRSQAEDSASTELPEN
                     LEPFLAAHPFVQSDH"
BASE COUNT         1739 a         2627 c         2472 g         1658 t
ORIGIN      
        1 gccctcaccg ccctggccgt atacatgctc accgtccgtt caggctcgtt ccggtggctg
       61 ctgctggcag tcccgttgac agtccttgcc acgctggccg cgccggaaaa cgccggactg
      121 gcccttgtgt gcgctttatt tgagaccgtg tgtgcgtcag attctgcccg caaacggata
      181 atgcgcctgg cgccagttct tctggcggtc ctgggagcgt cactgacgca tggcaaggct
      241 atgttcgcac atgatggtaa tgttgcggcc atgtttggac cgctgcattt gatcttgtat
      301 cccatcggct ttcttccaat taccgccagg tatattcagg aaattccagt gctggggtgg
      361 cttatggcag tgcctgtggt cacgttgatc gccctcatct attggaaagc ccggcggccg
      421 atgattctgt tcggccttct ggcgatgcct gcggtcagga tctttggggg aggacgcccc
      481 ttcgagccgg tccatttgat tggcggcggc cagctgctgc ttgcgaatgc atttttcgcc
      541 atcgctctga ccgcgctgtt tcaccgcatc atggaccacc ccaaatggcg gcgcctgacc
      601 gtcagtggca cctctttgct atgcctgatt ttcatggttg tgcagatgca tgccctcgtg
      661 cagtggcgca gtgcgggaaa cctgctgcgc gattttcaca cgcagctccg gatgatgaag
      721 aatgagactt tggagcccgt cttcgaagaa gcgctgctga tttctcccga tattcattac
      781 gttgaagggg cgccggtgtg tttgtctgcg tccctcatga ttggcgacca aaacgtaccc
      841 taccggcctg atgtgcccac cttttctgga ggcgtgtttg gtgggccaaa aataaacatc
      901 ttcaggcgaa aaggcgtcta tgtgcagagc agtatcgaag atgatcgtgt gattgctgtg
      961 gttgaagggg caacccactt tgacgtcgtc cctctcgagg actgctggaa atggaatgtg
     1021 gaacggggcg gaagagatcc tataatttgg gcagaactcc ccgaaggaaa ccctcatctg
     1081 ctggtgtttc ccgggcagtt tccctggcct tcccctgaac cgtagtgcct gcacgcagat
     1141 ggaataaaac ctgcactgcg caagcctgat tgccctggat catgcccggg gccaagccga
     1201 tgtgaacacg cttctgaggg catcgctgcg atgagcgagc gttgatcgat cgctgctgct
     1261 gccgtaaaat gctgcgagat aacttgcacc tgaagaggaa cttccgtgga gtcaaactcc
     1321 agcaacccgg ctcatcgctc ggacacgccc ggaacctgtc ccgcctgtgg aggcagaaca
     1381 gcggcggagg actttttctg ccgccgatgc ggacggagcc tgtcgctcgg ctcgaaatgg
     1441 tattacgatc cgctgtggat ctgggttctc gggctatttg ttcttggccc gctcgcgctt
     1501 cctctggcat ggcggtcacc gaaaatgccc gcgctgcaca agatcacgtt caccgtggcc
     1561 gtgaccgccc tgaccaccct tcttgtgatc ctctgctaca tcgtcttctc gtacatttgg
     1621 agcagccttg ctgccatccg cgaagcgggc cgtgcactgt ggccataatc gccccgcggg
     1681 cgcatctgct ggtcagaggg gaatcgacag ctggcagacg ttgcttcttt ccgcaaagat
     1741 ccccagggcg ttgaacaaca aattggcctt gaagcggtgc ccgaactcgt tcggatgaat
     1801 caggtcattc atgaggtagt gcagattgct tttccgggcg tattcgttga aggccgcaaa
     1861 atgatccacg agaattgcgc cgctttgttc ggcaacttcg cgcatgacca cgacgtaaga
     1921 ttcgagattc gaggcacgct gagcctccgt gcagggcagg acgggattgg gcgtgtgaat
     1981 cacgatggcg gcgccgcatc ccgctcgcac ccgctcgaca acgtccagat attgctcgcg
     2041 gaagcggggg agggcttcgg ggccggcctg gcagtcgttc atcccgaaat gaaacgaggc
     2101 cacctggggc gcgaactgca ggacccgcca atccaggtct tccgcgagcc gcccgagggt
     2161 ccacccgctg gtggccgtgc gaattaccag gttgcgctcc tggcccagct cataacggac
     2221 acgttcttcg aacaattgcg ggtaatcccg ccagccatac gtgtgcaggg cgccgtgtgt
     2281 gatgctgtct cccgcaaaca cccatttgat cgcctggttt ccttcaagaa acccggcgac
     2341 ccgggccaat tcatccgcca cagcgggtgc ggggttgcct gctgtctggg actgagcgct
     2401 ttccccggcc gcaactgcca ccgtcaacac cgcagcgctt ttgatggccg ttctgcgtga
     2461 gattttgtct gtcatgttgc ctccttcagc acacgtaaat cgggtttcaa cgtgggacta
     2521 tttcgctgga gcagacgcgg ccacgccacg ccactggtaa ctggattgga cgtagtcggt
     2581 caggcgtttt tgaagttcag ccacgagtgc tttctgttcg gatgcaatgg ttgcccggcg
     2641 cgttgaggcg cctacgcggt acagggccga ttcgccggtt ccgggcgccg gcaattcgcc
     2701 aaacgcagac gggcccggtt cccacacgta tcgccagttc ccggatcgca ccgacaatcg
     2761 aaacggtttg gccatcatgg ataccgggtc tttcccgacc gggccaaaaa gaaagctccg
     2821 cccttccaaa gcggcctcgc gggaaagtcc ggcaacgcgg gaaagagcgg gcgcgatatc
     2881 ttccagaccg accaggtact cccgcttctc cctgaccatt tccggcgcgt agtagaggat
     2941 cggcacccgc atgtcgtctt cggtaagctc cggcgccagt tgcccgccgt cggcgccgaa
     3001 cgagaccccg tacggcgaaa aaagaaggat gctggtgtag tcgcggtagg ccgaatcccg
     3061 gatatgctgg atcagccctc caatcagggc atccatcgac tcgagcgctg cgtcgtacaa
     3121 ctccgtgtct tttgccggcc cgggctgatt cgacgggcgg gcgccaaggc gttcacggct
     3181 tttcgcgtcg gccaggtcgc cggcgcgcac aaacagcata aacgccacgt cctggtgctc
     3241 gtccatccac gcgcatgcct gctcgacggt acgctggctg ccttcctcat cctctgcata
     3301 tttcgcgtcg aaacactcaa atccattttc aaatccgctg ccgaactcca ggtcgccccg
     3361 gtattcgccc tctgtaaacg ccgccgtagc gtaatggttt tcgcgaagga gcgcggcaag
     3421 cgtcctggcc ttttcaggca gaggcccctg tgctccgccg aaatatccgt ggcaaagggg
     3481 attcactccc gtaaaacacg tcatgtacgc ggcgctggca tccggagcgg ggctgtaccc
     3541 gtatcgaaag gcgacactgc tgtgcacgag gcgatccagc tgaggcgtag tgcggcgccg
     3601 gtatccccag gccgacatgc ggtctgagct caaaccatca atgccgatca aaacgaggtt
     3661 gggcggcgtt ttttcggcgt gcgtgcggtg cagcagaggt ccggacatca gcaactggcg
     3721 gctggagtgc accaccggca aaaatcccag caaacgcatc caggtgggcg aggggctttg
     3781 tgtccagaac accgcgcagc gggttgctcc agcgggaatg gcatccgccg gcacacgcag
     3841 cgtggcccag gcgctctctg agaggctcac agcggcctca tacttgtctt tttcttcgcc
     3901 ttcaatcacc acggtcacat aggcgcgccg cacggcttca tcgggggtgg catccgggag
     3961 atttgcccag cgcagcgtaa tcccccggtt ttcatagatc ggcaggtcat agcgagccat
     4021 tgccccttcg ggaatcgggc gggcctctgc atacgcccct cccgcccggt aaatgcccgg
     4081 catcgaggcg gcctccggcc gggcagcctc gattcccgca tgccattggt gcaacgcgat
     4141 gatcgtggcg ccggccgcca cacccgccgc ccccagccac gccaggacgg gtaatcgcct
     4201 cgagggttcg ctgtacagtg ccgtatagaa ggagaacagt ttcagcgcgg cgtgcaacgc
     4261 ggcatatccc cctagaaaca gcaggggttc tgccttggcc agcgccggat cgggccagcg
     4321 aatcgggatg tgcaggaggt acgggatcaa gacgaccgcc gcaaggtgag aaaggcattc
     4381 cgaaagcaga aactttcgtg cccgcgtggg tttaagcgct ttgaccagcg caacccaggc
     4441 cagctgtacc gtaatgtacg ccgcggcccc tccaaaagcc agccgggtgc tcaattcgaa
     4501 cgtcggcgca aaccccagtc gcgccaacag gctgtggaca aacgcgcctg ccactgccga
     4561 agacaggccc aacgtcagta catataccgc gctcacgtcg cccggaccgt gctcctatgt
     4621 tgtgacgtcc ccaagcctct ggtatgatcg atgccgcttg cagaaaatca tggttacggg
     4681 tcggcatagt accactcgtg accgtgcgga ggccaatcag gcatgttgaa ttcagttttg
     4741 cgctcactct ttggaacaag cagtgagcgg ctcctcaaaa aagtcgcccc tttgctcgac
     4801 gaagtccgtg cacacgaagc gcggatgctg gccctgacca acgaccggtt gcgggcaaag
     4861 accggagaat tcaaggagcg cctggctcag ggcgccagcc tcgatgacat ccttccggag
     4921 gcctttgcgg ccgtctgcga agccgcaaaa cgcgtcgtgg gcatgcgccc cttcgacgtc
     4981 cagatcctcg gcgggatcgt cctccacagc ggctccatag cggaaatggt caccggggaa
     5041 ggcaaaaccc ttgttgccac catgcccgtg tatctcaacg ccctgcccgg cgacggcgtg
     5101 cacgtcgtca cggtcaacga cttcctggcg cggcgcgatt cggaatggat ggggcccatc
     5161 taccagttta tgggcctcac gaccgggctc atccagcacg atatgggcac cgccgaacgc
     5221 caggtcgggt accgcgcgga cctgacctat ggcaccaata acgaattcgg ctttgactac
     5281 cttcgcgaca acatggccat ccgccctgaa atgcgggtgc agcgcaaact caactatgca
     5341 attgtggacg aaattgactc cattcttatc gacgaggcgc gtacccccct catcatctcc
     5401 ggaaggccgg aaaaaagcac cgacatctat tacaaagtgg acgatgtggt gcgccgcctc
     5461 cgcaaggaca cacattacga aattgaagag aagggccacc acgtactgat caccgaagag
     5521 ggcatgaaca ccctcgagac gctcctcggc gtggaagacc tgttcacaga acacctgggc
     5581 ctcgtccata tggtcgaaca ggcgctccgg gcgcacaatt tctacaaacg ggacgacgaa
     5641 tacgtggtgt acaatggcga agtcctgatc gtcgacgagt ttaccgggcg catgatggag
     5701 ggtcggcgct actccgacgg actgcatcag gcactcgaag ccaaggaacg cgtcgcggtg
     5761 cagtttgagt ctcagacagt cgccacgatc acctaccaga actattttaa gctgtacaac
     5821 aaacttgccg gcatgaccgg taccgccgcc accgaagcct ccgaattcgc ccagatctac
     5881 gaactcgaag tcgttcagat cccgaccaac ctcccgctga tacgcgaaga ccttcccgac
     5941 ctcgttttcg ccaccgaaga gggcaaattc cgccatgtgg tgcgcgagat tgccgaaatc
     6001 cacaagaccg gacgtccagt gctcgttggc accgtttcca tcgaaaaatc cgaaagagtc
     6061 tcgtccatgc tcgccgaaat gggcgtcacg gacttccagg tcctcaacgc caaatatcac
     6121 gaaaaagaag ccaccatcat cggaaacgcg ggcaagcgcg gcgccattac cattgccacc
     6181 aacatggccg gccgcggcac cgacatcaaa ctgggcgacg gcgtgcgcga accaggcggc
     6241 ctggccattg tcggtaccga acgccatgaa tcacgccgca tcgacaacca gctccggggc
     6301 cgttgcgggc gccaggggga tcccggaaat acccggttct tcctcagcct cgaagacgag
     6361 gtagcccgcc tgttcggcgg cgataaagtc aagcgcctgc tcgattggtt cggcagcaac
     6421 cagatggatg acgaacccct cagccagaaa atggtctcgc gctcgatcga gcgggcccag
     6481 cgccaggtcg aggaatacaa cttcgagatc cgcaaacacc tcaaagaata cgacgacgtg
     6541 atgaacaagc aacgcatgat catctacgac atgcgccgca atgtgctcga agaccgtgat
     6601 gtcaccgaac agctgcatga gatgttcgaa aacatcgtgg gccggctcgt caacgaattc
     6661 gcgtcagacg acgttctccc cgatgaatgg gacctcgaag gcctttccac ggggttccgg
     6721 gccatttttg gattcgagtt ttcggaggac atccaaccgt ccgggagcgg cggagcggag
     6781 ccccggccgc tgtttgaaga actcgttgcc caggtcgtga aggaatacga aggccgggaa
     6841 cacgtgcttg aggacgaaat tcgggcgcat taccgaagcg agatcggcgg cgacgaatct
     6901 caggtcgatt ttgcccgtat cgcccggaaa cgcacgcacg acctcgaaat gatggcgttg
     6961 ttgcgcgccg tcgatgaaaa atggattgac catctgtatt ccatggatta tttgcgagat
     7021 tcagtgcgcc tgcgcgcgta tgggcaaaaa gacccgctcg tggagtacaa aaccgaggca
     7081 ttcgagatgt ttcagaccat gatggcctcg attgaagaag aagtcgtgca gaccctcttc
     7141 cgcctctcgg atcccgaggt gcgccgccgc cgccgcagtc aggcggagcg cggcgtcctt
     7201 acccccgcgg aagacccatt cgccaagctg tcgcagtact cgtatgtgca ggccgacaaa
     7261 cagcaggacc gcagctttgc atcgtttgac acaagccgtt ttgccctggc cgggcaaccg
     7321 gacagtgaac agcagccgcc tggagagagc ccccgtccac gcgtaagaca acagcccatc
     7381 cgcagagcag ggactgagat caaacccaac gacccctgcc cctgcggcag cggcaagaaa
     7441 ttcaagaagt gttgcggcgc catcgcaaga aactgatcgg agcggccatt gtcctctttt
     7501 ggctgctgat gatgggcctg ctgggccgcc gcgaaattct gcctttgctg cgcgggccct
     7561 ctttgagcac caattatccc accagtccga cggacagctg gatgatggtc ctgtctccag
     7621 gcgagcagcc tcttggattc atcaacacgc agaccgcacc cgaaacccgc gacggactgc
     7681 ccggaaccct gctcaccctc aatgccatcc tgaaaatgca gtttctgggc cagcccatgc
     7741 ggatgcacct cggagggtcg gcctggtttg cacggacggg tgatgccggc cgcttcgatt
     7801 tcgcggttcg ttcggaagag cacagcgcgc gttttgaggg cgtgcttgga aacggcgtcc
     7861 ttaatgccag gatccacacg gcgggcgagg tgtatcccct ttcgctgcct gtgaaccgcg
     7921 aactcgccat cgcgggcatc gcaggcactt ccgccctcca gctggatcgt ctcaaacccg
     7981 gggaagaagc cgtaatcgag gccttcgatc ccatgaccct ctccatgggt acggcgcgtg
     8041 tgaaatgcct gggattcgac accctgggag agggagacga agcccgaaaa gtccgtgtgg
     8101 tctccatcac ctcgcacaat atcgagtcaa aagcctggct cgacgaagag ggacagaccc
     8161 tgcgcgcgga gacgccgctg ggcctcatct tgcagcagac ttcgtccagc gaagccctct
     8221 cggcggtatc ccgtgaagtc cctcaggatc tcatccaggc cgtcgcgatt catccatcgg
     8281 gcaaacggcc ggtgcgaggg gcaaacctga tgcggatacg aatctcgggg atccccgacg
     8341 ccgtgaccat tcctgaagat gccaaccagc gccggacagc agatggggat ctcctcattg
     8401 cgccgagatc gcaggctgag gatagcgcat cgaccgagct tcccgagaat ctcgagccgt
     8461 ttctcgcggc acatcccttc gttcagtctg atcatc
//
LOCUS       DAOPBQ010000081         8475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7788_length_8475_cov_3.969359, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000081 DAOPBQ010000000
VERSION     DAOPBQ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7788_length_8475_cov_3.969359"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PKW60_04035"
     CDS             complement(<1..993)
                     /locus_tag="PKW60_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98629.1"
                     /translation="MSTRDNRSVTARPSLNGKRSPSEAVRRIFPVSGIALLLGVYLLY
                     DAISYTRPGPATTLAYAHSALMLAVAAVNLRWGVYFFVISVVSADEISRYLWEFTGDT
                     GVTSFLTVSVGGVALSNLVALGLIAIAAVLSIVRFAQHPRGRRLLPADWALLTIGLVY
                     LIASLHGVRNLFNEPRLAINHLNLPLMLLGLYFVVRFAFREPLQVFHFWRVALLAMAV
                     KVIGWTILAFTGEGSLFGTTLRVGFGAGWNLFVFVLLLGVIFVIHGDAFRLLDRFIAA
                     VLAILAGGLLLVNAGRMLWVFSALGLVLLLIFDKARAKLYALGFSALCGIAIVFL"
     gene            complement(1042..2313)
                     /locus_tag="PKW60_04040"
     CDS             complement(1042..2313)
                     /locus_tag="PKW60_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPJ98630.1"
                     /translation="MFELLQTRTVRNLSLLTAVNFISGILRFLCTILLAKILAPGDWG
                     NVAIFISILDVVFILADAGLNATLVRFVAAHPDKRAASVVGRCLGLRILLSAGLFLGL
                     WAFQGPILAKQHFPPELHWVYLFSVFSAIALAFHAMSLSIFQAKEEYGRYSISFLSVN
                     VIRASGLALFVLLGKGTLNALILLYFSAPFIGLLLVFPFAVASMQRTASLPPSPVKMK
                     EIVLFMLPLAAMSAITIGHMRVSSFMLKMLATPEAVANYELAYQIGFALPLVTRAMIT
                     VLLPRVSTMETGDELLRYRSRVLRLYFIILPLTLAGVLLAPLFISLVFGEKYVESLNI
                     IRLLIAAFGISVLMPPLGLVFYAMKRPIYLTGVQLLQFVITIALNYYFIPRWEGIGAA
                     VVMLIGTLVSAFAIITLSSRVIRRSPEPDPA"
     gene            2686..5154
                     /locus_tag="PKW60_04045"
     CDS             2686..5154
                     /locus_tag="PKW60_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPJ98631.1"
                     /translation="MLSGGKQGRIASIILCAGKGRRVQSPRTPKVCFPVAGKPAICHL
                     LEALESRGSAPNILVVGHLAGTVVDEVGPKFPAALFAYQANLLGTGHAARQGASVLSG
                     LEYTGPVLVLAGDKLVEPRTLDKLIATFERDRPDVALIVAPKNRWPNAGRIVTDSNGR
                     LLQIIEKADLVKASQEGSTFQVAGQTRTAEDIENSVEWVNQAVYLFRAPVLYKALAEL
                     RRDNVQKEEYLTDTIDYAVSRGLTVTPVPVDDPDDVLGFNSPPELLEIEEHFRKKLGL
                     DVAEQVALDPAVFKPAETWAQVLSNPDGAVSEMLRSIYGDNASLCEEKRGQLLRTVEN
                     FIERYGADGPVTVIRAPGRINLMGRHVDHRGGNVNLTAIDREHILVARPRRDSLVRAH
                     NLDENAFEDLEFSVDDLLRQVRLDQWRDFVDSETVLKMVSDLQGNWGNYLKAPMLRLQ
                     ERFKDRRIYGVDCVVSGDIPMAAGLSSSSALVVAMGEALVLSNGLDVTPNDLVYLCGE
                     GEWFVGTRGGSADHAAVKLSQFGQVVTVGFFPFVIRGYVPFPEGYSLIIANSRIQARK
                     AAGAREAFNERVASYELAVHWVRKLFPNYAPLIAHLRDISPESLGIRSADVYRILLDV
                     PETASADTLRRTIGGDAFDQITLMHSPRESYPLRARLLFGIAECERSKMLHRLLLEGD
                     IKRLGWMMNISHDGDRVSTADGTDYLPPLDDAYLNACISNLQSEDPDRVFAGQLYAQA
                     GSYACSIPQIDRMVDIALETPGVLGAQISGAGLGGCMMILTESQGAEAVVERLTRHYY
                     EPNGLEPGASAFVPIAGCSHLRLS"
     gene            5249..7132
                     /locus_tag="PKW60_04050"
     CDS             5249..7132
                     /locus_tag="PKW60_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPJ98632.1"
                     /translation="MYKDWGQLGDIARLFVQLKPNFKWITLVSLVVALLAGAYVALTH
                     QPTYLSEAIAIVRPQQVPLSRSRKDEAQPREEKGKSLLPQPLNVTDYVLFLQSDGMLA
                     SIAQAYNAYHAKESEPLLTVAQLRRMMMPNARLEIKTPYAVEYYPTIELRVSAQAPDK
                     AYELARLWVETARDWSKTVTFSAKEDMADYLKNETEEQRSSLRDLSGELNRTKDESGE
                     LLEALRAERLRAEEEYESETIRLLQETGDTWDANIAAAEAEFALPLMETELQARESIL
                     AGLRLAQARKTLELERARAAITELTTQTGAADALDSIATSWDIALPSEKEAIAPVAPP
                     MEKTEALREEELEKRAEKRLLVDSERRENIRTLLKDYTKSPHRRLQDEHEKRFEDILE
                     MLDKQLPPPKERPEPEKSPEIPELDLETPVQLDAFRVETEGGNPVGILLQKQLAEARL
                     TLASAPVELQHIEREIASLRSDLDALQADYHAKRKALVMLDRAKQSDLDRIDKERLYG
                     LMREQRVTEMKVDELSREPTALEEALERDFATGLDMFTSLAASHLEAELAVANTIDEF
                     QVVSPPTFPESPPRRQYIVYGGGAFVACFSVLFVLAAFVVILRTLIRNIQTSEAPDTA
                     TPD"
     gene            7197..>8475
                     /locus_tag="PKW60_04055"
     CDS             7197..>8475
                     /locus_tag="PKW60_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPJ98633.1"
                     /translation="MHDIILTTLNARYSHTAFGLRYLWANLGPLQPRAAIREFVIGQE
                     PAEIAEALLLERPRIIGIGVYIWNIEPIEKLVQILTSVSPETVIVLGGPEISYEYEDT
                     ALYRMAGYLVPGEGELAFRELAEAVLSGEPPRQKVFAGGRPELESLALPYTAYSDHDI
                     AHRTLYVETSRGCPFKCEFCLSSLEPGVREFPLPPIFEALNALFARGARQFKFVDRTF
                     NLREDRVLAMLDFLQARLCDEMQVHFEIVPDRLPVRVLERMTEFPPGVLRLEVGVQTF
                     NLQTQDAISRVQDLDATLATLQFLRERTGALLHADLVLGMPHETWDSIRDGFDRLLAV
                     RPQEIQVGILKRLKGAPIARHTKPHEMIYSTSPPYEILQTATLDFLQMQRLKRFARYF
                     ELYYNSGNFPASLPALWADQSSPFEAFMAFSDFL"
BASE COUNT         1887 a         2515 c         2556 g         1517 t
ORIGIN      
        1 tagaaagacg attgctatgc cgcacagcgc ggaaaagccg agggcgtaga gctttgcccg
       61 cgctttatcg aaaatcagca gaagcaccag gcctaatgcg gaaaagaccc acaacatgcg
      121 cccggcattg accagcagca gcccaccggc gagtatggcc agcaccgccg cgatgaaccg
      181 gtcgagcagg cgaaacgcat ccccatggat cacgaaaatc accccgagga gaagcacgaa
      241 aacaaagaga ttccagcccg caccgaaccc cacgcgcagg gtggtcccaa acagggagcc
      301 ttcgcccgtg aacgcgagta tcgtccagcc gatgaccttg acggccatgg caaggagcgc
      361 cacccgccag aaatggaata cctgcagggg ttctctgaag gcaaagcgca caacgaaata
      421 gaggccgagc aacatcaggg gcagattaag atggttgatc gcgagccggg gctcgttgaa
      481 caaattacga acgccatgga gtgaggcgat cagatacacc aggcctatcg tgagcagagc
      541 ccagtcggcg ggcaaaagtc gccggcctct cggatgttgc gcgaaacgga cgatgctcag
      601 gaccgcggcg atggctatga ggcccagagc cacgagattg gagagcgcaa caccccccac
      661 ggagaccgta agaaagctcg ttacgccggt atcgccggtg aattcccaca gataacgaga
      721 aatttcgtcc gcggacacca cggagatcac aaaaaaatag acgccccatc gcagattcac
      781 cgccgcgact gccagcatca aagcactatg agcataggca agcgtagtcg ccgggcccgg
      841 cctggtatag ctgatggcgt cgtatagcaa atacacgccc agtaacagcg cgatacccga
      901 caccggaaaa atacggcgaa ccgcttcgct cggcgaacgt ttgccgttca gagatgggcg
      961 cgcggtcacg ctcctgttgt ccctcgtgct cactcgaaaa ccattctcat gtctgcctgt
     1021 cgacagggcc cggacatcgc gtcatgcggg gtccggttcg gggcttcttc gaatcaccct
     1081 cgatgaaagc gtgataatag caaatgcgga gaccagggtg ccgatgagca tgaccacggc
     1141 ggcgccgatg ccctcccagc gagggatgaa gtaataattc agggcgatgg ttatcacaaa
     1201 ctggagcagt tgaaccccgg tcagataaat gggccgtttc atcgcgtaaa acacgaggcc
     1261 caggggcggc atgaggacgc tgatcccgaa tgcggcaatg agcaaccgga taatgttgag
     1321 cgattcgaca tatttttccc caaacaccag ggaaatgaag agtggcgcga gcaagacgcc
     1381 cgcaagcgtc aggggcagga tgatgaaata gagccgcagc acgcggctcc ggtagcggag
     1441 cagctcgtcg cctgtttcca tggttgaaac gcgcggcagg agaacagtga tcatcgcgcg
     1501 ggtgaccaga ggcaaggcaa acccgatctg gtacgccaat tcgtaattgg ccacggcctc
     1561 cggcgtggcc agcatcttca gcatgaagct gctcacccgc atgtgcccga tggtgatcgc
     1621 gctcatggcc gcaaggggca gcatgaacag aacgatttct ttcattttca cgggcgatgg
     1681 cggaagcgat gcggtgcgct gcatgcttgc gaccgcgaac gggaagacga gcagtaagcc
     1741 gatgaacggg gcggagaaat acaacaatat cagcgcgttg agggtcccct tgccgagcag
     1801 aacaaacaac gcgagaccgg atgcgcgaat gacgtttacg gatagaaaag aaatcgagta
     1861 gcggccatac tcctccttcg cctgaaaaat agagagagac atcgcatgga atgccagggc
     1921 gattgcagaa aagaccgaga agagatagac ccagtgcagt tccggcggaa aatgctgctt
     1981 ggcgagaatc ggcccctgaa atgcccataa acctagaaat aaaccggccg acagaagaat
     2041 tctaagcccc aggcagcgcc cgacaactga ggccgcccgt ttgtcaggat gcgcggcgac
     2101 aaaacgaacc agggtcgcat tgaggcccgc gtcggcgagg atgaacacca catccagaat
     2161 ggagatgaat atggcgacat ttccccagtc cccgggcgca aggattttgg ccaaaaggat
     2221 ggtgcacaga aagcgcagga ttccagagat gaaattgact gctgtcagga gggacaggtt
     2281 gcgtacagtg cgcgtttgca gcagctcaaa cactgcgcgc actctctctt taaatgcatc
     2341 cagaatgctt gttcctcact ccccggtacg actccgggcc gcacgattca tctctcgagg
     2401 gccatcggag ggcctgaaaa gacacccggc acggcgcgcc gggtacgcca cttcccgcgc
     2461 ggcgaatgca cggcgcaacc tcccccggcc tgaatacgtt gtacccgtgc aaatactatc
     2521 cccacggcat cgacaaatca agaaacggtc acgacttacg aggccgggcg gcctgccgtc
     2581 aagcgcggtg cattcgcagt gcgccagaag attgtgtata ctctgacagc agtgaagaac
     2641 agcacccaca ggcttccgca gcgggagaag tgagggaggt attccgtgct ttcagggggc
     2701 aaacaggggc gtatagcatc aatcattctg tgtgcgggga aggggcggcg ggtgcaatcg
     2761 ccaaggacac cgaaggtgtg cttccccgtg gcgggaaaac ccgccatctg ccatcttctc
     2821 gaagcactgg aatcgcgggg cagcgccccc aacatcctgg tggtgggcca tctggcgggc
     2881 acggtggttg atgaagtcgg gccgaaattt ccggccgccc tgtttgccta ccaggcgaac
     2941 ctgctgggga cgggtcacgc tgcgcgtcag ggagccagcg tgctgtccgg actggaatac
     3001 accgggccag tcctcgtgct ggccggcgac aaactggtcg agcctcgcac actcgacaag
     3061 ctgatcgcga cctttgagcg ggaccggccg gatgtcgccc ttatcgtggc gccgaagaac
     3121 cgctggccca acgcaggccg catcgtgacc gattcaaacg gcaggctgct ccagatcatc
     3181 gaaaaagccg atctggtcaa ggccagccag gaaggaagca catttcaggt cgcgggccag
     3241 acgcgaaccg ccgaagacat cgaaaattcc gtcgaatggg tcaatcaggc cgtctatctg
     3301 ttccgcgcgc cggtgctcta caaggcgttg gccgagctcc gccgtgacaa tgtgcagaaa
     3361 gaggaatacc ttaccgacac gattgactac gcggtctcgc gcggactgac ggtcacgccg
     3421 gttcccgttg acgaccccga cgacgtcctg ggattcaaca gtcctcccga actgctggag
     3481 atcgaggagc atttccgaaa gaagctgggg ctggatgtcg cggagcaggt cgctctggat
     3541 ccagccgtat tcaagccggc cgagacctgg gcgcaggttc tctcaaaccc cgatggggcc
     3601 gtgtcggaga tgctgcgctc catttatggc gacaatgcaa gcctgtgcga agagaagcga
     3661 ggccagctgc tgcgcacggt agagaatttc atcgagcggt acggtgcgga tgggcccgta
     3721 acggtcattc gcgctcccgg ccgcatcaat ctgatggggc gccatgtgga ccaccggggc
     3781 ggcaatgtca atctgacggc catcgatcgc gagcatatcc tggtggcacg cccgcggcgt
     3841 gattcactgg tgcgcgcaca caatctcgac gagaatgcct tcgaagattt ggaattctcc
     3901 gtggacgacc tgcttcgcca ggtccggctc gaccagtggc gcgattttgt tgacagcgaa
     3961 acggttctga agatggtgag cgacctccag gggaattggg gcaattatct caaggccccc
     4021 atgctccggc tgcaggagcg cttcaaggat cgccgcatat acggggtgga ttgtgtggtg
     4081 agtggcgaca tcccgatggc ggccggtctg agctcctcct cagccctggt cgtggcgatg
     4141 ggcgaggcgc tggtgctttc caacggtctg gacgttaccc cgaacgatct ggtctacctg
     4201 tgcggagaag gcgaatggtt tgtcggcacg cggggaggca gcgccgacca cgccgcggtc
     4261 aagctcagcc agttcggtca ggtggtcacc gtgggcttct tcccgtttgt gatccgcggc
     4321 tacgtacctt ttcccgaagg gtattcgctc attattgcga attcacgaat tcaggcgcga
     4381 aaagccgcag gcgcacgcga ggcgttcaat gaacgcgtgg cttcctacga gctggcggtg
     4441 cactgggtgc gcaaactgtt cccgaactac gccccattaa tcgcccacct gcgcgatatc
     4501 tccccggaat ctctggggat acgctcagcc gatgtctacc gcatcctgct cgatgtgcca
     4561 gagaccgcca gcgcggacac gctccggcgg accattggcg gcgacgcctt cgaccagatc
     4621 acgctgatgc attcgccccg cgagagctat cccctgcgcg cacggcttct gtttggaatt
     4681 gccgagtgcg agcgcagcaa gatgcttcac cgattgctgc tcgaaggcga catcaaacgc
     4741 ctgggatgga tgatgaacat ctcgcatgat ggcgaccggg tctcgacagc agacggcacc
     4801 gactatctcc cgccgctcga cgacgcctat ctgaacgcgt gcatcagcaa tctgcaaagc
     4861 gaagatccgg acagagtgtt cgcgggacag ctttatgcgc aggcgggctc atacgcctgc
     4921 agcatccctc agatcgatcg catggtggac atcgcactgg agacacccgg cgtacttggc
     4981 gcgcagattt ccggggccgg tctgggcggt tgtatgatga tattgacgga gtcgcagggc
     5041 gctgaggcgg tggtcgagcg gttaacccgc cattactacg agccgaacgg cctggaaccc
     5101 ggcgccagtg cgtttgtccc gattgcgggc tgttcgcatt tgcgcctttc ttgaagaaca
     5161 ggctcgcgag tcctaaacta ctgctaatac agggcttgcc tggacaggcg cggtaatcgc
     5221 gcctggcagg acgatgccgg aggtgattgt gtacaaagat tggggccagc ttggcgatat
     5281 tgcacgtctg tttgttcagt tgaaacccaa cttcaaatgg ataaccctgg tgtctctggt
     5341 cgtggctctt ctggcgggcg cctacgtcgc gctcacccac cagccgacct acctcagcga
     5401 agccatcgcg attgtacggc cgcagcaagt accgctaagc cgttcacgaa aagatgaggc
     5461 gcagccccgc gaggaaaaag gcaaatccct cctcccccag cccctcaacg tgacggatta
     5521 cgtgctgttc ctgcaatcag acgggatgct ggcgtcgatc gcgcaggcgt ataacgccta
     5581 ccatgcgaag gaatcggaac cactgctcac ggtggcgcaa ctgcggcgca tgatgatgcc
     5641 caatgcccgc ctggagatta agaccccgta cgcggtcgag tattatccca cgatcgaact
     5701 gcgcgtctcg gcgcaggccc cggataaggc ctatgaactg gcccggctat gggtggagac
     5761 ggcgagagac tggtcgaaaa ccgttacgtt ctcggcaaaa gaggatatgg ccgactatct
     5821 caagaacgag accgaggagc agcgttcctc actccgggat ctgagcgggg aacttaaccg
     5881 caccaaggac gagagcggcg agcttctcga agccttgcgg gccgagcgtc ttcgcgccga
     5941 agaggagtac gagagcgaaa ccatccggct tctccaggaa acaggagaca cctgggatgc
     6001 gaacatcgcg gcggcagaag cggaatttgc cctgccgctg atggaaacgg aactccaggc
     6061 ccgggaaagc atcctcgccg gcctccgcct ggcccaggcg agaaagaccc tggaactgga
     6121 acgggcccgc gctgccataa ccgagctgac cacgcagacc ggggcggcag atgcgcttga
     6181 ttccatcgcg acgtcatggg acatcgcgct ccccagtgaa aaagaggcga ttgcgccggt
     6241 ggcgccgccg atggagaaaa cggaggcttt gcgcgaagaa gagctcgaaa aacgcgcgga
     6301 aaagcgcctg cttgttgatt cggagcgccg ggagaacatc cggacgcttc tgaaggacta
     6361 caccaagagc ccgcatcgca gactccagga tgaacatgaa aaacgctttg aagacatcct
     6421 ggaaatgctg gataagcagc tgccaccgcc caaagagcgc cctgaacccg aaaaaagtcc
     6481 agaaattccc gaactggacc tcgaaacgcc cgtgcagctc gatgcctttc gtgtggagac
     6541 ggaagggggc aatcccgtgg gcatccttct tcaaaagcag cttgctgaag cgcgtttgac
     6601 gcttgcatcc gctccggtcg aacttcagca tatagagcgc gaaattgcca gcctccgctc
     6661 cgacctcgac gctctgcagg ccgattacca cgccaaacgc aaggcgttgg tgatgctgga
     6721 ccgtgcgaag caatccgatc tcgaccgcat cgacaaagag cgcctctacg gcctgatgcg
     6781 cgagcagcgc gtcacggaaa tgaaagtgga cgagttgtcg cgtgaaccca cggcactgga
     6841 agaggcgttg gagcgcgatt tcgcgaccgg tctggatatg ttcacgagcc tggccgcaag
     6901 ccatctcgag gcggaattgg cggtggccaa caccattgac gagtttcagg tcgtttcgcc
     6961 ccccacgttc cctgaaagtc cgccgcggcg gcagtacatc gtttacggag gaggcgcgtt
     7021 tgtggcctgc ttctccgtct tgttcgtgct ggccgcattc gtcgtgatcc tgcgcacgct
     7081 catccggaat attcagacca gtgaggcccc tgatacagcc acaccggact gatccgtcgc
     7141 agcggcggcg taacgcagca aacggaagcc cgggtccgca aaatttcacc gcaaacatgc
     7201 acgacatcat cctgaccacg ctgaatgccc ggtacagcca tacggctttt ggcttgaggt
     7261 atctctgggc caatctcggg ccgcttcagc cccgcgccgc catacgcgag ttcgtcattg
     7321 gccaggaacc ggcagaaatt gcagaggcgc tgctgctcga gcgcccgcgg atcatcggca
     7381 tcggcgtcta catctggaat atcgagccga tcgaaaagct cgtgcagata ctcacctcgg
     7441 tgtcgcccga gaccgtcatt gtgctgggcg gcccggaaat cagctacgaa tacgaagaca
     7501 cggcactcta ccggatggcc ggctatctcg tccccggaga aggggaactg gcgtttcgcg
     7561 agctggcaga ggcggttttg agcggggagc ccccgcgcca gaaggtattc gctgggggac
     7621 gccccgaact ggagtcgctg gctctcccct acacggcgta ttcagaccat gacatcgccc
     7681 accggacgct ttatgtggag acatcgcgcg ggtgcccgtt caaatgcgag ttttgcctgt
     7741 cgtcgctgga gcctggtgtc cgtgagtttc ccctcccccc cattttcgag gcgctgaatg
     7801 ccctgttcgc ccgtggggcg cggcaattta agttcgtcga tcggacgttt aacttgcgtg
     7861 aagatcgcgt gcttgccatg ctggactttt tgcaggcccg tctttgcgac gagatgcagg
     7921 tgcattttga aattgtgccg gatcgcctgc ctgtgcgcgt cctcgagcgc atgacggaat
     7981 ttccacccgg cgtgttgcgc ctggaagtgg gtgtccagac tttcaatctt cagacccagg
     8041 acgccatatc gcgggtgcag gacctcgatg cgactctggc cacgctgcag ttcttacgag
     8101 agcgcacggg cgcgcttctt catgccgacc tcgtgctcgg catgccgcac gaaacctggg
     8161 acagcattcg cgacgggttt gaccggctgc tggccgtacg gccacaggaa atccaggtgg
     8221 gcatcctgaa acgcctgaaa ggcgcgccca tcgcccggca tacaaaacca catgagatga
     8281 tctactccac ctcgcctccc tacgaaatcc ttcagaccgc cacgctcgat ttcctgcaga
     8341 tgcaacggct gaaacgcttc gcgcgctatt tcgagctcta ttacaattcc ggcaattttc
     8401 ctgcgtccct gccggctctc tgggcggacc aatcctcccc gttcgaggcg ttcatggcct
     8461 tctccgactt cctgt
//
LOCUS       DAOPBQ010000082         8464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7798_length_8464_cov_4.655250, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000082 DAOPBQ010000000
VERSION     DAOPBQ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7798_length_8464_cov_4.655250"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PKW60_04060"
     CDS             complement(<1..279)
                     /locus_tag="PKW60_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98634.1"
                     /translation="MKPVCAMLLLFAAGALLPACDRGKGPAGEPTRSVPDAPDNSAAP
                     PPAPGARLTEFFFYRASAGGVEQRPSFVMRTPAVSITAQGEALLEEVEA"
     gene            complement(276..1052)
                     /locus_tag="PKW60_04065"
     CDS             complement(276..1052)
                     /locus_tag="PKW60_04065"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006038116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPJ98635.1"
                     /translation="MLLVMDVGNTITAIGLYEGPELRAHWRSVTGNWRTSDELRVFLN
                     MLLQDEGLEPEMVNGCCVSSVVPSLNAALTDLCANHFEAELVFVEPGVKTGLVIRVDN
                     PREVGADRIVNAVGALESHEGPLIVIDFGTATTFDAVTARGEYQGGIIVPGIKIAADA
                     LFEKCAKLPSVEITKPAAVIGKNTIDHIRSGLTYGYADLVDGLILRMANEMDEKPLAL
                     ATGGFAELVAGIATRIDIVDPYLPLKGLRAVFLKNRKGAA"
     gene            complement(1040..1795)
                     /locus_tag="PKW60_04070"
     CDS             complement(1040..1795)
                     /locus_tag="PKW60_04070"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPJ98636.1"
                     /translation="MSDPPEKEAFFSRPLHTRVVGSRILVFDTVDSTSSRAFNSGLDG
                     TVVVAEHQTQGRGRLGRIWHSPPGLGLWFSVAFTGGVDGLEFAAPLAVRDGLRPYCVL
                     TLKWPNDLLADSRKICGILVERRAGITVAGIGINVRHRPEDMPPELRATAGSIFMATS
                     QTPERSEVLERVLTALDHRVCALRAGQGELIRREWAAACGVEGRRVRYRGIEGFVTAI
                     DGLGGLILDTPAGVSRVLFGEPLEWMEDELCCS"
     gene            complement(1788..2621)
                     /gene="nadC"
                     /locus_tag="PKW60_04075"
     CDS             complement(1788..2621)
                     /gene="nadC"
                     /locus_tag="PKW60_04075"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPJ98637.1"
                     /translation="MLEFRRLMREALAEDVGQGDITTRSMVSKDARCRVRLVAKQAGV
                     LSGIEVFRTVFDLLKARVRRWEALEDGARFVAGDRVAGFEANTAAVLTGERLALNFVQ
                     HLSGIASITRQYVDALNGLEVRICDTRKTTPLMRALEKQAVMHGGGFNHRFALFDGVL
                     IKENHIVAAGGVRAAVEKAIRGTHHLMKVGVEVTSLDELQEALAAGADSILLDNMSLE
                     DMAKAVKMTANHQVILEASGNVTLERVRAIAETGVHYISIGALTHSAPSVDLTLLIEN
                     V"
     gene            2793..3842
                     /locus_tag="PKW60_04080"
     CDS             2793..3842
                     /locus_tag="PKW60_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HPJ98638.1"
                     /translation="MQVALYILGMGLGVLLLAKSADWFTDAAVSIACRLNVPEILIGA
                     TLVSLATTLPEFAVSVAAALKQQTDVAVGNAVGSTIANIGLVLGLCIVLSPMGVARRG
                     FLRSSLGLVFVCLIFACIGYLFPEGSRWTGVVMLLCLAAYLAANIRSAMPDPKPGTSR
                     IPTAGESSRDALLGELASETSVDGAQRVELATGLTAGRVAAYFLLGAVGVIAGSKLVV
                     YCAEHLALAAGISRHVIALTLVALGTSTPELVISLAAIIKKQRGLSIGNIIGANILNL
                     VWVLGASSLITVIPLDAQSLYFDIPVMLFLTGLLFVFGFTGERLSRWEGFVLLSVYAA
                     YLVVLFTVYRVPAVA"
     gene            complement(3924..4907)
                     /gene="galE"
                     /locus_tag="PKW60_04085"
     CDS             complement(3924..4907)
                     /gene="galE"
                     /locus_tag="PKW60_04085"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPJ98639.1"
                     /translation="MALLVTGGAGYIGSVATELLLSAGERVVVLDNLSRGHRGAVAPE
                     ATFIEGDIRDRALLDSLFNQHEIDTIMHFAAFALVGESVEKPALYFENNVNGSHRLLN
                     AAVDAGVKRFIFSSTCATYGFPDRVPLTEDLPTRPINPYGLSKRMVEEILEWYATAYD
                     FQYVALRYFNACGATESHGEDHAPETHLIPNIFRAITGEGGPLQVFGDDYNTPDGTCI
                     RDYVHVTDLIDAHIRSVNYLREGGASDYFNLGTETGNSVLEVIRAVERVTGVTVPYTI
                     APRRAGDADRLVASSAKAREVLRWQPAKRDIELVVSDAWRWHQAHPRGYGA"
     gene            complement(5028..6137)
                     /gene="ugpC"
                     /locus_tag="PKW60_04090"
     CDS             complement(5028..6137)
                     /gene="ugpC"
                     /locus_tag="PKW60_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPJ98640.1"
                     /translation="MARVELKSVRKVYPGNVEAVKDANLSIQDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEVSDGEIYIGDTLVNDVPPKNRDIAMVFQNYALYPHMTVYKNMAFGLM
                     LRKYPKAEIEQRVREAAQILGIEHLLDRKPKALSGGERQRVAVGRAIVRQPKVFLFDE
                     PLSNLDAKLRVQMRAEISKLHTRLQSTMIYVTHDQVEAMTMGDRIVVMLDGVIQQIAP
                     PLELYHHPVNRFVAGFIGSPPMNFLEGELSAENGTLRFHDKSRTLSLDVHETHRAALE
                     NYVGGKIILGIRPEHFLENLQQQAIPGRSMTANVEVVEPMGHEVYLYLDVGGQSLTAR
                     IPVGETEPAVNKPHVLDIDTQGIHFFDAQTESAIT"
     gene            complement(6285..7037)
                     /locus_tag="PKW60_04095"
     CDS             complement(6285..7037)
                     /locus_tag="PKW60_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98641.1"
                     /translation="MVSIGIVLILIAILLLLAATQLKTVARSGIERTLSFAFLTDVSV
                     ESVDLVLQEGHVELHNLTVNNPASFRSGPAMELGTIVADLDLKTLFSATPTLRSLVIR
                     DVRVNLRHELAEGANLVMLARHASRFSKPDGAAGETADKSTSKRPARAAREFIIQELR
                     CEGATVLMSSNLIPLSRVQVEVAPFTITEFSNNRPVSAADLSAIFIRSLIRQTLSTKG
                     LLRPIADLLRGEEDAGDSSTPLIEEPEPQLRE"
     gene            complement(7096..7848)
                     /locus_tag="PKW60_04100"
     CDS             complement(7096..7848)
                     /locus_tag="PKW60_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98642.1"
                     /translation="MRLGRLWILCALLCVVGAACQTTRSAPPDAEAPLLSAPRGYQPM
                     SYVSTIQMNEGEYPDLFSAESSAIWVDNDVTEMKYERERETGQTPPAGLVAAARAVNR
                     DFFVFECHIESVFRDTSIAYDVVGFRGIDVHLETPDGKQIEPIQIIIGTPVEEEQRGT
                     LKQFRRINIAVFPRTDLWIGGDTVAVGSGAVRLVLSGHGSTFCFEWPELPQASTDTGR
                     SWNPSENEMVQAVKVSFTDLYGRLRRLAHTFD"
     gene            8015..>8464
                     /locus_tag="PKW60_04105"
     CDS             8015..>8464
                     /locus_tag="PKW60_04105"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPJ98643.1"
                     /translation="MTDTDRLDSLDAPADDSPPPPDSTNAFTGSDYALATLGALLALA
                     VYLKTLALTVTGEDSGELIAAAATLGIPHPTGYPLWCLLGFAFTKIIPFGSVALRVNC
                     MSAFFGAATVFLVILIVIRLTRSHLAGLGGGLSLAFSREFWEQSVIAE"
BASE COUNT         1502 a         2668 c         2607 g         1687 t
ORIGIN      
        1 cgcctcgacc tcttccaaca aggcttctcc ctgcgccgtg atcgacacag caggcgtacg
       61 catcacaaag gagggccgct gctctacgcc gcccgcgctc gcccggtaga agaaaaactc
      121 ggtcagccgc gccccgggtg cgggcggagg ggctgctgag ttgtccggcg cgtccggaac
      181 gctgcgcgtg ggctccccgg ctggaccttt tccccggtcg caggcgggca gcagcgcccc
      241 cgcagcaaac agcaggagca tggcgcagac cggcttcatg cggccccttt ccggtttttc
      301 agaaacacgg ctcgaagtcc cttcagcggc aggtacggat ccacgatgtc gatgcgtgtg
      361 gcgatgcccg ccacgagttc cgcgaatcct ccggtggcca gggcgagcgg tttctcgtcc
      421 atttcattcg ccatgcgaag gatgaggccg tctaccaggt cggcgtagcc gtaagtgagc
      481 cctgagcgga tgtggtcgat cgtattcttc ccgataacgg ccgccggctt cgtaatctcg
      541 acgctgggga gtttcgcgca tttctcgaac agggcgtcgg cggcgatctt gatgccgggc
      601 acgatgatgc cgccctggta ttcccctcgc gcggtgaccg cgtcgaaggt ggtcgcggtg
      661 ccgaaatcga tcacgatcag gggaccttcg tgcgattcaa gcgcgccgac ggcattcaca
      721 atgcggtcgg cgccgacttc cctggggttg tcgacccgta tgacgaggcc ggtcttgacg
      781 cccggctcga caaaaaccag ttctgcctcg aagtggttcg cgcacaggtc cgtcaaagcc
      841 gcattgaggg atggaaccac gctggacacg cagcacccgt tcaccatctc cggctcgagg
      901 ccttcgtcct gcaacagcat attcaaaaag acccgcaatt cgtcgctcgt acgccaattt
      961 cccgtgacgc tgcgccagtg ggcccgcagt tcgggccctt cgtacagccc gatggcggta
     1021 atggtgttgc ccacatccat cacgagcagc atagttcgtc ctccatccat tcgagcggtt
     1081 cgccaaacaa gacccggctc acgcccgcgg gcgtatcgag gatcagcccg cccagcccgt
     1141 cgatcgcagt cacgaagcct tcgattccac ggtaccgtac gcgccggccc tctactccac
     1201 aggccgccgc ccattcccgc cggatcaatt cgccctgccc cgcccgcaac gcgcagaccc
     1261 ggtgatccag cgcggtcaac acgcgctcaa gcacctcgct ccgctccggc gtctggctcg
     1321 tcgccatgaa gatggaaccc gcggtggcgc gcagttcggg cggcatgtct tcgggccggt
     1381 gccgtacgtt gatcccgatt cctgcgaccg tgatgccggc ccggcgctca accaaaatac
     1441 cacaaatctt gcgggaatcg gccaggagat cgtttggcca tttgagggtg agcacacaat
     1501 aggggcgcaa gccgtcgcgc acggccagcg gcgcggcgaa ttccagcccg tccacgccgc
     1561 ccgtaaacgc cacgctgaac cacaagccca ggcccggggg gctgtgccag atccgcccaa
     1621 ggcggccgcg gccctgcgtc tggtgttctg ccacgacaac cgtcccgtcg aggccgctgt
     1681 tgaacgcccg cgaactggtg gagtcaacgg tgtcgaaaac gagaatgcgc gagcccacca
     1741 cccgggtgtg gagcgggcgt gaaaaaaacg cctccttttc aggagggtca gacattttcg
     1801 atgagcaacg tcaaatccac cgagggcgcc gaatgcgtca atgccccgat ggaaatgtag
     1861 tgcacgcccg tctcggcgat ggcgcgcacg cgctcgaggg ttacgttgcc gctggcctcg
     1921 agaatcacct ggtgattcgc cgtcatcttg accgctttgg ccatgtcctc cagactcatg
     1981 ttatccagca ggatcgagtc cgcgcccgcc gccagcgctt cctgcagctc atccaggctc
     2041 gtgacttcaa cgcccacctt catcaggtgg tgcgtgccgc ggatggcttt ctccaccgcc
     2101 gcacggaccc cgcccgcggc gacaatgtgg ttttccttga tcaacacccc gtcaaacagg
     2161 gcaaagcggt gattgaaccc tccgccatgc ataaccgcct gtttttccag cgcgcgcatc
     2221 agcggcgtgg tcttacgcgt atcgcagatt cgtacctcga gcccgttcag cgcatcaacg
     2281 tattgccggg taatgcttgc gatgcccgac aggtgctgca caaaattgag cgcgagccgc
     2341 tcgccggtca gcaccgcggc cgtgtttgct tcgaacccgg ccacccggtc tccggccaca
     2401 aaacgggccc cgtcttccag ggcctcccag cgccggacgc gagccttcaa caggtcgaac
     2461 accgtgcgaa acacctcaat accactcaat acgcccgcct gcttagccac caggcgcacc
     2521 cggcagcgcg catccttgga taccatcgat cgtgtcgtga tatccccctg gccgacgtct
     2581 tccgcaagcg cctcgcgcat taatctgcga aattcaagca tcttgtgtcc tttctggtgg
     2641 attagccggg gatcttaaca cgaagtctgt tccaaaagcg acgtgaccag tgtctggggt
     2701 ttgtgccgat gaggttttcg gtggcgcggc cgccgccggt tcctgtctgc gggcagatcc
     2761 cttgttccgg ccgtgctata ctcccgccca caatgcaagt agcgctgtac atccttggaa
     2821 tgggcctcgg ggtgctcctg ctggccaaat cagcggactg gtttacggat gctgcggtca
     2881 gcattgcgtg ccgcctcaat gtgccggaaa tcctcatcgg ggccacgctg gtaagtctgg
     2941 ccacgacgct ccctgaattc gccgtttctg tcgccgcggc gctgaagcag cagacggatg
     3001 tcgcggtagg caacgccgtg ggctcgacca ttgccaacat cggcctggtt ctgggcctgt
     3061 gtattgtgct gtcgcctatg ggggtggcac ggcggggatt cctgcgcagc agtctgggcc
     3121 tggttttcgt gtgcctgatc ttcgcgtgca taggttactt gtttcccgaa ggttcgcgct
     3181 ggaccggggt ggtgatgctc ctttgtctcg cggcgtatct cgcagcgaat atccgttcgg
     3241 ccatgccgga cccgaaaccc ggcacttcac ggattccgac cgccggagag tcgtcccgtg
     3301 acgctctgct gggggaactg gcctcggaga cgtccgtgga cggcgcgcag cgcgtggaac
     3361 tcgccacggg tctcacggcg ggccgggtcg ccgcttattt tctcctgggc gccgttggcg
     3421 tcatcgcagg cagcaaactg gtcgtctatt gcgccgaaca cctggcgctc gcggcgggga
     3481 tctcacgaca cgtgattgca ctgaccctgg ttgcgctggg cacgtccacg cccgaactgg
     3541 tgatcagcct cgcggcgatc atcaaaaaac agcggggatt gtcgattggc aacatcatcg
     3601 gggcgaatat actgaacctg gtgtgggtgc tcggcgccag ctcactgatc accgtgattc
     3661 ccctcgatgc gcagagtctg tatttcgata ttccggtcat gctgttcctg accggactgc
     3721 tgttcgtgtt cgggttcacg ggcgagaggc tgagccgctg ggaagggttc gtgctgctga
     3781 gtgtgtatgc cgcctatctg gtggtcctgt tcaccgtata ccgcgtcccc gccgtagcct
     3841 gacatccgct cgggttcgca ggcggcgcgt gcttggagag cggctgagta tgcctgcggc
     3901 gcctgtactg gaacgccggg gcttcacgct ccgtatccgc gaggatgcgc ctgatgccag
     3961 cgccaggcgt cgcttaccac gagttcgatg tcccgtttcg cgggctgcca gcgaagcact
     4021 tcacgggcct tggcggatga ggccaccagc cggtccgcgt cgcccgcccg ccgcggcgcg
     4081 atcgtgtacg gcaccgtgac gcccgtgacg cgctcaaccg cccgaatgac ctccagcact
     4141 gaattgcctg tctcggtgcc gaggttgaaa taatcggatg ccccgccttc gcgcaggtag
     4201 ttgacggaac ggatgtgggc gtcaatcaga tcagtcacgt ggacataatc gcggatgcag
     4261 gtgccgtccg gggtgttgta atcatcgccg aacacctgga gcggtccgcc ctcgccggtg
     4321 atggcgcgga aaatgttggg gatcaggtgc gtctccggcg cgtggtcttc gccgtggctc
     4381 tccgtggcgc cgcaggcatt gaaataccgc agggctacgt actggaaatc gtacgctgtt
     4441 gcataccact cgaggatttc ttcaaccatc cgcttcgata aaccgtaggg attgatcggg
     4501 cgcgtgggca gatcttccgt cagggggact cgatcgggaa acccgtaggt cgcgcaggtg
     4561 gagctgaaaa tgaagcgttt taccccggcg tccacggcgg cgttcaacag cctgtgggag
     4621 ccgttgacgt tgttttcgaa atagagcgcc ggtttttcaa cggattcgcc caccagcgca
     4681 aacgccgcga aatgcatgat cgtgtcgatc tcgtgctgat tgaaaagcga gtcgagcagc
     4741 gcgcgatccc ggatatcgcc ttcgatgaat gtcgcctccg gggcgactgc cccgcggtgc
     4801 cctcgcgaca gattgtccag cacgaccacg cgctcgcccg cggaaagcag cagctccgtg
     4861 gccacgctgc cgatataccc tgcgccgccc gtcacaagaa gcgccatggc attccttcct
     4921 ttcgctcaag cggttcctgt ccgccgttct ggaatgacag ccccgtatgg ggctccgttc
     4981 gcctggtgcg aaagcgcagg tgagctgtgg tcaacgcgcc cgggcgctca ggtgatggcc
     5041 gattcggtct gggcatcgaa gaagtggatt ccctgggtgt cgatgtccaa gacatgcggc
     5101 ttgttcaccg cgggctcggt ctcgcccacc gggatccggg ccgtaaggga ctggcctccc
     5161 acgtcaaggt agaggtagac ctcatggccc atgggctcca cgacttcgac gttcgcggtc
     5221 atgctgcggc ccggtatggc ctgttgctgc aagttctcaa gaaaatgttc ggggcggatg
     5281 cccaggatga tcttgccgcc cacataattc tccagcgcgg cgcgatgcgt ctcgtgaaca
     5341 tcgaggctga gcgtacggct cttgtcgtga aaccggagcg tcccgttctc cgccgacagc
     5401 tcgccttcaa ggaaattcat gggcgggctg ccgataaatc ccgcgacaaa gcggttgacg
     5461 ggatggtggt acagctccag tggcggcgca atctgctgga ttaccccgtc cagcatcacc
     5521 acgatacggt cgcccatcgt catggcctcg acctggtcgt gcgtcacgta gatcatggtg
     5581 gattgcagcc gcgtgtgaag cttgctgatc tcggcgcgca tctggacccg gagcttggcg
     5641 tcgaggttcg agagcggctc gtcgaacaag aatactttcg gttgccgcac gatggcgcgg
     5701 cctacggcca cgcgctggcg ttcgccgccg gacagcgcct tgggtttgcg gtccagcaga
     5761 tgctcgatgc ccaggatctg ggccgcctcg cgcacgcgct gctcaatctc ggccttggga
     5821 tacttccgca gcatcagccc gaacgccatg ttcttgtata ccgtcatgtg cggatacagg
     5881 gcgtaattct gaaacaccat ggcgatgtcg cggttcttgg gcggcacgtc gttcacgagg
     5941 gtgtcgccga tgtagatctc gccgtcggag acctcttcaa ggccggcgat catgcgtagc
     6001 gttgttgatt ttccacagcc cgaagggccg accagcacaa tgaattcttt atcctgaatg
     6061 ctgaggttgg cgtcttttac tgcctccaca ttgccgggat acacttttcg gacgcttttg
     6121 agttctaccc tcgccatggt gtagaggcct cccaactgag tgtggataaa gtcatatcgc
     6181 caaggcagat cgcgacattc ctcacggtgc aattttggat aataccccac cgtccgggag
     6241 cagtcaagga cagccgcggg ccctgaaaac gctgacgagc gagcctattc acgcagttgc
     6301 ggctcgggtt cttcgatgag cggagtgctg gaatcgccgg cgtcttcctc gccccgcagc
     6361 aggtcggcga tggggcggag cagccctttt gtcgacagcg tctgccggat gagcgaccgg
     6421 atgaaaatgg cgctgaggtc cgccgcagag acgggccggt tgttgctgaa ctctgtgatg
     6481 gtgaacgggg caacttcgac ctgcactctc gagagcggga tcagattgct gctcatgagc
     6541 accgtcgcgc cctcgcagcg gagttcctgg atgatgaatt cacgggccgc gcgggcgggc
     6601 cgtttcgagg tcgatttgtc cgccgtctct cctgcggcgc catccggttt cgagaaccgt
     6661 gaggcatgcc gcgccagcat caccagattg gcgccctcgg ccagttcgtg gcgcagattc
     6721 acgcgaacat cccggatcac cagcgaacgg agcgtgggtg tcgcggaaaa aagggttttc
     6781 aggtcaagat cggccacaat cgtgcccagt tccatggccg ggcctgaacg aaagctcgcg
     6841 gggttgttga ccgtcaggtt atggagctca acgtgtcctt cctgaagcac gaggtccacg
     6901 ctttcgaccg aaacatcggt cagaaaggcg aaactcaacg tgcgttcgat accgctccgg
     6961 gctacggttt tcagctgtgt ggctgccagg agcagcagga tggcgatcag gattaagaca
     7021 atcccgatgc tgaccatcaa tgttttcaga caggatcgca ttgcacgctc agtgtccgcg
     7081 tcgcgcgaac cgggctcagt cgaaggtatg cgccaggcgc cggagacggc cgtacaaatc
     7141 agtaaacgag actttcaccg cctgcaccat ttcgttctca gacggattcc aggagcggcc
     7201 ggtatctgtg gaagcctggg gcagctcggg ccactcaaag cagaacgtcg agccgtggcc
     7261 ggaaagaacc agccgcacgg cgccgctgcc tacagcaacc gtgtcgcctc cgatccagag
     7321 gtccgtccgg ggaaatacgg cgatgttgat ccggcggaac tgcttcagcg tgccgcgctg
     7381 ctcctcctcg accggcgttc cgatgatgat ctgaatgggc tcgatctgtt ttccgtcggg
     7441 cgtctccagg tgcacgtcaa tgccccggaa tccaaccaca tcataggcaa tgctcgtatc
     7501 gcgaaatacc gattcaatgt ggcattcgaa cacgaaaaag tcgcggttta ccgcgcgcgc
     7561 cgccgctacc aagcccgcag gcggcgtctg gcccgtttcg cgttcgcgct cgtatttcat
     7621 ttccgtgacg tcgttatcaa cccagatggc ggaagattcc gcgctgaaca ggtccgggta
     7681 ctcgccctca ttcatctgga tcgtggacac atacgacatc ggttgatagc cccgcggggc
     7741 ggaaagaaga ggcgcttccg cgtccggcgg cgcgctccgg gtggtctggc aggctgcgcc
     7801 cacgacgcac agcaacgcac acaggatcca caagcggccc agtctcatga cagatcctcc
     7861 ctttccaacc ttcggcgtat cctatcacag gcgcagggaa ccggtaaaag atcatcgcgt
     7921 ggcgggcagc caaccggcgc cgcagttttg gcctcggctg acgtgcccgt gcgctccgtt
     7981 ttatgcaacg cgaatgaatg ggtatcatac acgcatgaca gacaccgaca ggctcgattc
     8041 actggatgcg ccggcagacg attccccgcc gcctccggac tccacaaacg cgtttacggg
     8101 gtcggattat gcgctggcca cgctgggggc gctgcttgct ctggcggtct atctcaaaac
     8161 gctggcgctg accgtgacgg gcgaagacag cggggaactg atagccgcag ccgccacgct
     8221 gggcatcccg cacccgacgg gctatccgct gtggtgtctg ctcggctttg cgttcacgaa
     8281 gatcatcccg ttcggcagtg tggcgctccg cgtgaactgc atgtcggcat ttttcggcgc
     8341 ggcgacggtg ttcttggtga ttctgatcgt gatccggctg acgcggagcc acctggcggg
     8401 gcttggaggg ggcttgtctc tggcgttttc acgggagttc tgggaacaga gcgtgatcgc
     8461 cgag
//
LOCUS       DAOPBQ010000083         8422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7840_length_8422_cov_3.789530, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000083 DAOPBQ010000000
VERSION     DAOPBQ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7840_length_8422_cov_3.789530"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1234)
                     /locus_tag="PKW60_04110"
     CDS             complement(179..1234)
                     /locus_tag="PKW60_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPJ98644.1"
                     /translation="MPKESMTPRERWLAVLSRREPDRVPMDIWLTPEACDLLCSHLGC
                     DVETALERLHIDTPLTVAGQYVGPTPPEGQDIWGLVRRHIDYGTGAYDEVVNAPLAQF
                     TSVEEIEANYVWPSPDWWDYAQVPALLRGNEHRPIRGGGSEPFLLYKQLRGEEQAFLD
                     LLMYPDIVHYCLDKLFELAYQDTLRLFETIPGQIMITYIAEDLGGQTGLLMAREHMHE
                     FLFPRMKRMMDLTRQHGSFVFTHSDGAVRDIIPDFIALGTQVLNPIQWVCPGMDRAGL
                     KRDFGAALVFHGGVDNQHVLPFGSVDDVREEVRENYRTLGAGGGYILCPCHNIQAVSP
                     PENVVALYETGYEEGWL"
     gene            complement(1274..1996)
                     /locus_tag="PKW60_04115"
     CDS             complement(1274..1996)
                     /locus_tag="PKW60_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98645.1"
                     /translation="MAKRLDFENALLNDIRKKRRKRPTVKLRWVLALFAVGIALAASA
                     SALLTLFGGNSHAPLAETAAGFAARIATEQMDAAVEYCAEGAMGSQLVASERARLYLP
                     GAAAAFLPDPNARANRMALLTRIHDDLARAGLDWQNAQMLAFGGVLAKVSDPARMQSA
                     ASVAVGNVYLDDGHGVYTIEMSFMNCLGSYVITDIWQWGALEVVPEALKAHSRALFET
                     FRKEPATDSAVVKAPEHVFIKR"
     gene            2323..3702
                     /locus_tag="PKW60_04120"
     CDS             2323..3702
                     /locus_tag="PKW60_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HPJ98646.1"
                     /translation="MAVHRIKKGLNLPITGEPRQEITPAAQPVRVALVAADYPGMRPT
                     MFVQEGDAVKRGQLLFEDKKTPGVRYTAPGAGQIAGIHRGERRALQSVVIELNEHERS
                     GALTDDDHVVFAAYQGKAPATLKREELVELLVESGLWTVLRTRPFGKVPAVDAVPHSI
                     FVTAIDTNPLAPSVEKVLQGREQDFGTGLEVVTRLTEGVTYLCQRPGDTIPVPANAEL
                     QIEEFEGPHPAGLPGVHIHLLDPVHRGKTVWYIDYQSVVSIGRLFTTGKLDVERVIAL
                     AGPMAKNPRLLQTRVGAATDGLVANELREGEARVISGSVLSGRQAEGDIHGYLGLRHH
                     QISVVEEGRHRPFLGWMMPGSDTFSMLNLFLSRLTPGKRFAFTTNTNGAERAMVPMGM
                     YERVMPMDIHPTYLLRSLIVHDLEEAEKLGCLELDEEDLALCTFVCPSKYEYGPILRE
                     NLDMIEKEG"
     gene            3702..4943
                     /locus_tag="PKW60_04125"
     CDS             3702..4943
                     /locus_tag="PKW60_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HPJ98647.1"
                     /translation="MKALRNILDKQARHFEKGGKLEALYPLWEAGDTFLYSLGKVTRT
                     ASHVRDGADLKRIMITVVVALLPCMFMAMFNTGHQAQLAVRQGAALWDGWQTTVFTAL
                     GFDLVHGGFIACMVYGALFFIPVYIVTLAAGGTAEVIFCIVRKHEVNEGFLVTSALFP
                     LILPATIPLWQVAMGILFGVVIGKEVFGGVGMNIFNPALTARAFLYFAYPMQISGDSV
                     WIPAQTSADGYSGATWLAVMKDAGMEGLRHGISDVRGGITWWESFIGLEPGSMGETSA
                     LFCLIGALILITTRVASWRIMLGGLVGSAGAVLLFNAFAQDSSSVFAVPVHWHWVLGG
                     FAFAIVFMATDPVSAPFTDTGRYLYGFLIGFLGILIREVNPAYAGSWMLSILFMNMFS
                     STIDHYVVRANIKRRLARNAA"
     gene            4933..5745
                     /locus_tag="PKW60_04130"
     CDS             4933..5745
                     /locus_tag="PKW60_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009022446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HPJ98648.1"
                     /translation="MQRSGSLYTLGFAGAVCVMCAVVVSSSAYFLRAPQERNKLLDRQ
                     RQILSVAGLLESGTVLPPGEVRKLFDDSVEKAFVNLKTGKALTSDEFRSETGLDPATY
                     DAREASTKSEFGRDAPEGNLARIPRLANYEIVYYIREEGQISEIILPINGKGLWSTLY
                     GFLALDKDTRTVRGITFYEHGETAGLGGEIENPKWKALWKGRLVYDENWIPDITVIKG
                     QAGPPEADPHEVDGLSGATITSRGVSNLVRFWLGEDGFKLFLEQFRNQGSAA"
     gene            5745..6380
                     /locus_tag="PKW60_04135"
     CDS             5745..6380
                     /locus_tag="PKW60_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HPJ98649.1"
                     /translation="MGIGLNKADQKVLLDPLFNNNPIAFQVLGICSALAVTTKLDTAI
                     TMSIALTAVTAFSNLSISLIRNHVPHTIRMIVQMTIIASLVIVVDQVLRAYMFDISKR
                     LSVFVGLIITNCIVMGRAEGYAMQHGPKASFLDGVGNGLGYSLILLILGFFRELFGNG
                     SLLGRQVLALKRDGGWYEPNGLFLVAPSAFFIIGLLIWALRTWKPEQREEE"
     gene            6393..7007
                     /gene="nqrE"
                     /locus_tag="PKW60_04140"
     CDS             6393..7007
                     /gene="nqrE"
                     /locus_tag="PKW60_04140"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HPJ98650.1"
                     /translation="MEHYLSLFAKSVFIENMALAFFLGMCSFLACSKKVETAVGLGIA
                     VVIVQSITVPMNQLVYNFLLRRGALTWLSAGLSEVDLSFLGFLTYIGTIAAAVQLLEM
                     TLDRYVPALYVALGVFLPLITVNCVIMAGSLFMVERDYTFAESAVYGLGSGTGWALAI
                     IALASIRERMKYSNVPLGLRGLGIAFILTGLMAIGFMTFSGIQL"
     gene            7054..8283
                     /gene="nqrF"
                     /locus_tag="PKW60_04145"
     CDS             7054..8283
                     /gene="nqrF"
                     /locus_tag="PKW60_04145"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HPJ98651.1"
                     /translation="MLIILLGMLMFNFVILSLVIILMIARSKLVSTGEVHIEINEDKD
                     KTLTVPAGASLLSTLASRQIFLPSACGGKGTCGVCKVKVIEGGGAMLPTESGHISRGE
                     AREGIRLSCQVKVKSDLKIEVPPEIFEIRKWECTVRSNRSVATFIKELVLELPEGEAV
                     PFRAGGYIQIDCPPHTVHYKDFDIEERFRDDWDKYGLWNLVSRCDEEVTRAYSMANYP
                     EEKGLIMLNVRIAPPPPRASQDIPPGFMSSYIFNLKPGDKVTISGPFGEFFARDTDNE
                     MVFIGGGAGMAPMRSHIFDLFKRLHTHRKVSFWYGARSLREAFYVEDFNAIQAENENF
                     KWTLALSEPMPEDQWDGPVGFIHQVLLDNYLGTHPAPEDNEYYICGPPMMNTAVINML
                     QDLGVEEGNILFDDFGL"
BASE COUNT         1591 a         2460 c         2671 g         1700 t
ORIGIN      
        1 atcttcctcc agaaacgaaa aatcgcgttg cgtgcactca gcacttgaat gtgagttcgt
       61 cttgagaatt tgcatttact tttcgcaaga aggcggggat ttgtgccggc cgccgttttc
      121 agcacggcag cgctgtcgcg ttggagtgtg gaaatcctgc tgtcgccctg cgtgagtctt
      181 acagccaacc ttcctcatag ccggtttcgt agagagcgac aacattctca gggggcgaca
      241 cggcctggat gttgtggcac gggcacagga tgtagccgcc gcccgcgccc agcgtacggt
      301 aattctcacg gacctcttcg cggacatcgt ccacggagcc gaaggggagc acatgctggt
      361 tatcgacgcc gccatggaac accagggcgg cgccgaaatc ccgcttgaga ccggcccggt
      421 ccataccggg acagacccat tgaatggggt tgagcacctg agtgcccagg gcgatgaaat
      481 cggggatgat gtcgcgcacg gcgccatcgc tgtgggtaaa cacgaacgag ccgtgctggc
      541 gcgtcaggtc catcatgcgt ttcatgcggg gaaacagaaa ttcgtgcatg tgttccctgg
      601 ccatgagaag gcccgtttgg ccgccgaggt cttcggcgat gtaggtgatc atgatctgcc
      661 ccgggatggt ctcgaaaagc cgcagggtgt cctggtacgc cagctcgaaa agcttgtcga
      721 ggcaataatg aacgatgtcc gggtacatga gcagatcgag gaaggcctgt tcttctccac
      781 ggagctgttt gtagagcagg aacggttccg aaccgccgcc gcggatgggg cggtgctcat
      841 ttccgcgcag gagcgcgggc acctgggcgt agtcccacca gtcggggctg ggccagacat
      901 aatttgcctc gatttcctcc acggaggtga attgtgcgag cggcgcgttg acgacttcat
      961 cataggctcc cgtgccataa tcgatgtggc gccgcactaa accccagatg tcctgccctt
     1021 cgggcggggt gggccccaca tactggcctg ccaccgtgag cggcgtatcg atatgaagcc
     1081 gttccagggc cgtctcaaca tcgcagccca gatggctgca tagcaagtcg caggcttctg
     1141 gagtgagcca gatgtccatc ggcacacggt cgggctcacg gcgcgagagg acggccagcc
     1201 agcgctcacg gggcgtcatg ctctcttttg gcacgaaagg ctcctcaacc gggatgcgcc
     1261 cataaagccc ccgttagcgt ttaatgaaca cgtgttcggg cgccttgacg acggcgctgt
     1321 cggtggccgg ctccttccgg aaggtctcaa acagcgcgcg ggaatgagcc ttgagggctt
     1381 cgggcacgac ctcgagggcg ccccactgcc agatgtcggt gatcacatac gagccgaggc
     1441 aattcatgaa cgacatttcg atggtataga ccccgtgtcc gtcgtcgagg tagacgttac
     1501 cgaccgctac ggaggctgcg ctctgcatgc gggccggatc ggacaccttc gcgaggacgc
     1561 cgccgaacgc gagcatctgg gcgttctgcc agtccagtcc cgcgcgcgcc aggtcgtcgt
     1621 ggatccgggt gagcagagcc atccggtttg cccgcgcgtt ggggtcaggc aggaaggcgg
     1681 ccgccgcgcc ggggagataa aggcgggccc gctccgaggc gaccagctgg ctgcccatgg
     1741 cgccttccgc gcagtattct acggccgcat ccatctgttc ggtggcgatg cgtgccgcaa
     1801 atcccgcagc ggtctcggcc agcggcgcgt ggctgttgcc gccgaacagg gtgagcaggg
     1861 cggaggcgct ggcggccagc gcaatgccta ccgcgaacag ggccaacacc cagcgcaact
     1921 tgacggtggg ccgtttccgt cgttttttgc ggatatcatt caacagggcg ttttcaaaat
     1981 cgagtcgttt ggccaaagga atcaggctcc ccgcgacgtt ctcttctgcg tattcctctg
     2041 ctggcggctt cggacaggcc aacagtcact acccgcaatt ccgggttggg tatagggtag
     2101 cgttatcgcg cgtaaatcgc aaccggcacc aggacgccgg tgcgcgattc ggcctattgt
     2161 aatccgggtg ccagctgtgg caaaatcgcg gggcttaatg tgtcgtaaag cctgattcca
     2221 gcctgatgcc gtagaggtgc ggtttgtacg gcgtttgtat tcgtgacgca tttcacgaca
     2281 gcgtcccggc gcaggcatga ctcacaagga atggtcgaac acatggcggt acacagaatc
     2341 aaaaagggcc tgaaccttcc cattaccggc gaacccaggc aagaaatcac tcctgcggcc
     2401 cagcccgtcc gcgtcgccct tgtcgccgcg gattatccgg ggatgcggcc caccatgttc
     2461 gtgcaggagg gtgatgcggt caagcgcggg caattgcttt tcgaagacaa gaagacgccc
     2521 ggcgtacggt atacggcgcc cggagcgggc caaatcgccg gcatccaccg gggcgagcgc
     2581 cgcgcccttc aatccgtcgt catcgagctg aatgagcacg aacgcagcgg cgcgttgacc
     2641 gatgacgatc acgtggtttt cgccgcatac cagggcaaag ccccggccac gctgaaacgc
     2701 gaggaactcg tggagctgct ggtggaatcg gggttgtgga cggtgcttcg cacgcggcct
     2761 tttggcaaag tccccgcggt ggacgcagtg cctcactcga tttttgtcac cgcgatcgat
     2821 acgaatccgc tggccccctc ggtcgaaaaa gtgctgcagg ggcgcgaaca ggactttgga
     2881 accgggctgg aggttgtaac gcgcctcacc gaaggcgtca cgtacctgtg ccagcggccc
     2941 ggcgacacga tcccggttcc cgcgaacgcg gagctgcaaa tcgaggaatt tgaggggcct
     3001 catcccgccg gactgccggg cgtgcacatt catctgctgg accccgttca ccgggggaag
     3061 acggtgtggt acatcgacta tcagagcgtg gtctcgatag ggcgcttgtt cacgaccggc
     3121 aagctggatg tggaacgggt gatcgcgctg gccggcccca tggcaaagaa cccgcgcctg
     3181 ttgcagacgc gcgtgggcgc cgcgacggac ggcctggtcg cgaatgaatt gcgcgaaggc
     3241 gaggcgcggg tcatctcggg ctcggtgctt tcgggccgcc aggccgaggg cgacatacac
     3301 ggctatctgg gcctgcgcca ccaccagatc agcgtggtgg aagaaggccg ccaccgcccg
     3361 ttcctgggtt ggatgatgcc gggttcggat acgttttcga tgctcaacct gtttctttcc
     3421 cggttgacgc cgggcaagcg gttcgccttc acgaccaata caaacggagc ggagcgcgcc
     3481 atggtgccca tgggcatgta tgaacgggta atgccgatgg acattcatcc cacgtatctg
     3541 ttgcgatcgc ttatcgtgca cgatctcgag gaagccgaaa aactcggctg cctcgaactg
     3601 gacgaggaag atctggcgct gtgcaccttt gtgtgtccct cgaaatatga atacggcccg
     3661 attctccgtg agaacctcga catgattgag aaggaagggt aatgaaagcc ttacggaaca
     3721 tcctggacaa acaggccagg cactttgaga agggcggcaa actcgaagcg ttgtaccctt
     3781 tgtgggaagc gggcgacacc tttctgtatt cgctgggcaa ggtgacccgg accgcttcgc
     3841 atgtgcggga cggcgccgac ctcaagcgca tcatgatcac ggtggtcgtg gcgctgctgc
     3901 cctgtatgtt tatggccatg ttcaacacgg ggcaccaggc ccagctcgcg gtgcggcagg
     3961 gcgccgccct gtgggacggc tggcagacga ccgtgttcac cgcgctcggg tttgacctgg
     4021 tgcacggcgg ctttatcgcc tgcatggtct acggcgcgct gttcttcatt cccgtctata
     4081 ttgtcacgct ggccgcgggc ggaacggcgg aagtcatctt ctgtatcgtg cgcaaacacg
     4141 aggtgaacga agggttcctg gtaacgagcg ccctgttccc cctgatcctt cccgcgacga
     4201 ttccgctgtg gcaggtggcg atgggaattc tgttcggcgt cgtcatcggc aaggaagtct
     4261 tcggcggggt cggcatgaac attttcaatc cggccttaac ggcgcgcgcg ttcctgtatt
     4321 tcgcctatcc catgcagatc tcgggcgaca gcgtgtggat cccggcgcaa accagtgccg
     4381 acggctacag cggggcgacc tggctggctg tcatgaagga tgcaggcatg gaaggcctgc
     4441 gccacggcat cagcgacgtg cgcggcggca ttacgtggtg ggaatcgttt attggcctcg
     4501 aacccggctc catgggcgaa acctccgcgc tgttctgcct tatcggcgcc ctgattctca
     4561 tcactacgcg cgtggcttcc tggcggatca tgctgggcgg cctcgtgggc agtgccgggg
     4621 ccgttctgct tttcaatgcc tttgcgcaag actcgtcgtc cgtgtttgcg gtgccggtgc
     4681 actggcactg ggtgctgggc gggttcgcct tcgccattgt gttcatggcc acggacccgg
     4741 tgtccgcgcc tttcaccgac acgggccggt atctctacgg gtttctcatc gggttcctgg
     4801 gcatcctgat ccgcgaggtc aacccggcct atgcgggcag ctggatgttg tctattctgt
     4861 ttatgaacat gttctcctcg accatcgatc actacgtggt gcgagcgaat atcaagagga
     4921 ggttggcgcg gaatgcagcg tagcggctcg ttatataccc tcgggttcgc tggcgccgtg
     4981 tgcgtgatgt gcgcggtggt agtgtcctcg tccgcgtatt tcctgcgcgc tccgcaggag
     5041 cgcaacaaac tgctggatcg ccagcgccag atcctgagcg tggcgggact cctggagagc
     5101 gggactgttc tgccgccggg agaggtgcgg aaattgtttg atgactcggt tgagaaggcc
     5161 tttgtcaacc tcaagacagg caaggccctg acttccgacg agtttcgaag cgagacgggg
     5221 ctcgatccgg caacgtatga tgcgcgcgag gccagcacga aatctgaatt cggacgggat
     5281 gcgccggagg gcaatctggc gcgcatcccc cgcctcgcca attacgagat cgtctattac
     5341 atcagggaag aaggtcagat ctcggagatc atcctgccca tcaacggcaa aggactctgg
     5401 tccaccctgt acggatttct ggcactggat aaagacacgc gaacggtccg cggcatcacg
     5461 ttctatgaac acggcgagac cgcggggctg ggcggggaga tcgaaaatcc gaaatggaag
     5521 gccttatgga agggccgcct cgtctacgat gagaactgga tccccgacat caccgtgatc
     5581 aaagggcagg ccggcccccc tgaggccgat ccgcacgaag tcgatggcct gtcgggggca
     5641 accatcacga gccgcggcgt cagcaatctc gtgcggttct ggctggggga agacggattc
     5701 aagctgttcc tggagcaatt ccgcaaccaa gggagcgcag cctgatggga attggactta
     5761 acaaagccga tcaaaaggtt ctgctcgatc cgctgttcaa caacaatcca atcgcatttc
     5821 aggtgctggg catctgttcc gccctggcgg tgactaccaa gctggacacg gccatcacga
     5881 tgtctatcgc gctgacggcc gtgaccgcct tttccaatct gtcgataagc ctcatccgaa
     5941 accacgtgcc gcacaccatc cgcatgatcg tgcagatgac gatcatcgcc tcgctggtta
     6001 tcgtggtgga tcaggtgctt cgggcgtaca tgttcgacat cagcaagcgg ttgtcggtct
     6061 ttgtgggact gatcatcacc aactgcatcg tcatggggcg ggcggaaggg tatgccatgc
     6121 agcatggccc aaaagccagc tttctggacg gcgtgggcaa tgggttaggc tacagcctga
     6181 tcctcctgat actggggttt ttccgtgagc tgttcgggaa cggcagcctg ctggggcgcc
     6241 aggtgcttgc gctcaagcgc gacggcggct ggtatgagcc caacgggctc ttcctggtgg
     6301 cgccgagtgc gttctttatc atcggtttgc tcatctgggc gctgcgcacc tggaagcccg
     6361 aacagcgcga ggaggaataa cggatgactg gcctggaaca ttacctgagc ctcttcgcca
     6421 agtcggtgtt tatcgaaaac atggccctgg cgtttttcct gggcatgtgc agctttctgg
     6481 cctgttccaa gaaggtggag acggcagtcg gcctcggcat cgcggtagtc atcgtccaga
     6541 gcatcaccgt gccgatgaac cagctggtct acaatttcct gctgcgccgg ggcgccctga
     6601 cgtggctgtc ggccggtctg agcgaagttg acctgtcgtt tcttgggttt ctgacctaca
     6661 taggcacaat cgcggcggcg gtgcagctcc tcgagatgac tctggaccgc tatgtgccgg
     6721 cgctctatgt cgcgctgggc gtgtttcttc cgctgatcac ggtgaactgc gtcatcatgg
     6781 cggggtccct gttcatggtc gagcgggact ataccttcgc ggagagtgcg gtgtatgggc
     6841 tggggtcggg cactggctgg gcgctggcga tcatcgcgct ggcatcgatt cgcgaacgga
     6901 tgaaatacag caatgtgccg ctgggccttc gcggtctggg catcgccttt attcttaccg
     6961 gactgatggc catcgggttc atgaccttct ccggcatcca gttgtaagac aggccgcccg
     7021 ggcggctcga gcaaacgcta ggaacttgtg actatgctga tcattctcct cggcatgctg
     7081 atgttcaatt tcgttattct cagcctggtg atcatcctca tgatcgcccg gtcaaagctg
     7141 gtgagcacgg gcgaagttca catcgagatc aacgaagata aggataagac gctcacggtt
     7201 cccgcaggcg cgtcattgtt gagcaccctg gccagccggc agatcttcct tccgtccgca
     7261 tgcggcggca aaggcacctg cggcgtgtgc aaggtgaagg tgatcgaggg cggcggggcc
     7321 atgctgccca ccgaatcggg ccacatttcg cgcggcgaag cccgcgaagg catccggctt
     7381 tcgtgccagg tcaaggtcaa aagcgacctc aagatcgaag tgccgcccga gatcttcgaa
     7441 atccgtaaat gggagtgcac ggtacgttcc aaccgcagcg tggccacctt catcaaggag
     7501 ctggtgctcg aactgcccga aggcgaggcc gtgccgttcc gtgccggcgg ctacatccag
     7561 atcgattgcc cgccgcacac cgtgcactac aaggattttg atatcgagga gcgattccgt
     7621 gacgactggg acaagtacgg cctctggaat ctggtctccc gttgcgatga agaggtgacg
     7681 cgcgcgtatt cgatggccaa ctaccccgag gaaaaggggc tcatcatgct gaatgtgcgc
     7741 attgcaccgc cgccgccgcg tgcgtcgcag gatatacctc ccggcttcat gtcgtcgtat
     7801 atcttcaacc tcaaaccggg cgacaaagtc acgatttccg gacctttcgg cgagtttttc
     7861 gcccgcgata ccgacaacga gatggtgttc atcgggggcg gcgcgggtat ggcccccatg
     7921 cgctcgcata ttttcgacct gttcaaacgg ctccacacgc atcgcaaggt cagcttctgg
     7981 tatggcgcgc gcagcctccg ggaagcgttc tatgttgaag atttcaacgc gatccaggcg
     8041 gaaaacgaga atttcaagtg gaccctggca ctctcagagc cgatgcccga agatcagtgg
     8101 gacggcccgg tcgggttcat ccaccaggtc ctgctcgaca actacctcgg cacacatccc
     8161 gcgcccgaag acaacgaata ctacatctgc gggccgccca tgatgaacac ggcggtcatc
     8221 aacatgctgc aggatctcgg agtcgaggaa ggaaacatcc tgttcgacga ctttggcctg
     8281 taatggcgcc gcacgcgttg gcgcgttctg aagcgcggcg cgatcgtaag aaagggcaac
     8341 ggtgagtccc gcagtacgca gaaggccccc tggatggcgc gccgtcatgg cgctggccgc
     8401 cgtcctggcc tgcatgggct gc
//
LOCUS       DAOPBQ010000084         8374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7892_length_8374_cov_3.329727, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000084 DAOPBQ010000000
VERSION     DAOPBQ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7892_length_8374_cov_3.329727"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PKW60_04150"
     CDS             <1..476
                     /locus_tag="PKW60_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPJ98652.1"
                     /translation="STAVPFTVSAHASEAELAADALAHSYAGRFGQVLEPITQYAGFD
                     WRTNIALVGGFMAKEVVVGTLGTVMAMGDVDPGQAQALSQRLAADPAWNPLKGFALMV
                     FVMVYAPCFVTMAMIRRESGSWKWALFSTGYSTMIALVLAVLIYQGGLALGLGIS"
     gene            828..1115
                     /locus_tag="PKW60_04155"
     CDS             828..1115
                     /locus_tag="PKW60_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HPJ98653.1"
                     /translation="MNYQGVILGAVAFLTIGLFHPVVIKGEYYFGARIWPVFLVLGLL
                     CVALSLLVDNTYGSVILGVVGFSSLWTIHEIKEQEERVARGWFPKNPNRSK"
     gene            1291..2247
                     /locus_tag="PKW60_04160"
     CDS             1291..2247
                     /locus_tag="PKW60_04160"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HPJ98654.1"
                     /translation="MRGAVFFQIAFFVAMGLCFGAWAAEDTPVNPFTLPGSWYKANLH
                     THTTVSDGDVDLPVRVEQYKSHGYDILAVSDHEKTSDVAPFCTEDFLLISGMETHPKS
                     PNPSMAYHLLCINVPLGLSFEKETPMEERLKVLREAGVFVVAAHPYWCGFTLDELLPL
                     REHGVAAMEVYNSTCRFNGRADSSVHWDHLLSRGWRIPAIAVDDVHSDNSLRMGWTMI
                     KAESLTLDAIMAALRAGAFYASTGPEFTDMRVEGDSVVVECTPVQKIQFYAPAPNGSQ
                     ILASTEPLTKGVFTMKRKLAYVRATITDANGKEAWSPVLYFD"
     gene            complement(2277..2750)
                     /locus_tag="PKW60_04165"
     CDS             complement(2277..2750)
                     /locus_tag="PKW60_04165"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPJ98655.1"
                     /translation="MPNSKPCFICGEENAAGVQTRFYVDADIVKATFTGKKHHCGFAD
                     VMHGGIVAALLDECMGWAVARKIHRMCFTGELTVRYLKPVPANRPLIACAECVKANRR
                     VSTARSWIMDDEGTEYARAEGRFIPLSAEDTLEVDDNLLYRGGEERVFDCLRETP"
     gene            complement(2764..3189)
                     /locus_tag="PKW60_04170"
     CDS             complement(2764..3189)
                     /locus_tag="PKW60_04170"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HPJ98656.1"
                     /translation="MSQATLIYDGDCPLCCRAAAWAGKHARRGALELLPCQHEDRARR
                     FPAVTREQCLAAIQLVTADGRVYSGEQALPQLLDLVAGWHWMARILRFPGIRHASPAA
                     YRWLAGHRYALAALAGHGRGTACGRNGGAPGNERNGCGQ"
     gene            3311..4018
                     /locus_tag="PKW60_04175"
     CDS             3311..4018
                     /locus_tag="PKW60_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98657.1"
                     /translation="MQLEFPWYSTCQLRETLEQRSGLRLELTLTDNVHNLLSVRPSGA
                     RGQVKVRLHHLFLAADDSVLDALATWISRPEAKRAGTLLDDFIQENHHKIRQAPDQVR
                     IIRIRTRGQCFNLKKLFDEVNQRHFSGTIKAAITWGRMPTVRPQRSIRFGSYSSEFKL
                     IRIHPFLDRPFVPDFFVRYIVFHEMLHAHLGVVETEDGRHLAHTPEFRALERAYPDYD
                     RAVAWQKNRSTMRRLFR"
     gene            4394..5035
                     /locus_tag="PKW60_04180"
     CDS             4394..5035
                     /locus_tag="PKW60_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPJ98658.1"
                     /translation="MSSAAFRGADLPANRAHCVDEVQKTDEELMVALREGDREALAEL
                     VRRYQRDIFRFCQHYLRDFELARELTQETFVRVYVARQRFDEARKFRPWVLCIARNLS
                     LNELKRKKPVRMELLEEYASVARGPDGELHRSADGIPDQELMEKERRDALARLLDSLD
                     AESREIVMLRFFQKMAAKDIAEVVGSTEGAVRTRIHRILKSLREKSLATKDHL"
     gene            5032..6630
                     /locus_tag="PKW60_04185"
     CDS             5032..6630
                     /locus_tag="PKW60_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98659.1"
                     /translation="MRDREYDISLDVLADGLEGRLSEAQLAELREARAASAEVRANYL
                     WLQRAAHDLELIGEESVKEAPEIDVVEAVLRAVDKAAASEKTARLAAREPQRPSFVFW
                     GRAALAAAVLAMVFGMVLELGGFMPPEPAAPAVSQVPELPVTPVEQMATALPGEVSGS
                     ERLDDAVERLAQEITEKLDTSSKHAGRGDFLATAAPVLSSVTQNDVVALRQAAISEPG
                     AWVRLKRMATLDAATAAEVLENPDLPPDAMVGVAASLPDEMARQWLVTAVGRMDQQKP
                     YARLALATTYVTHAAPTADLPAPALFDGMSESDLRAFLAEMAALQEADPENALLDALQ
                     AGALLRLGDTEGALAALANLQGKGVATAYGLDAARSRQLALAAAGMPQESARMLSAVL
                     AGSDQYNMLCDLARDLVQSGQAYEAQGDPDTAQRIYEATQRLGEQVTGNTRLSEESLA
                     GIDIQRMAMEGLEALYANRGLASEMERLTSSALALVSQINNLGGMLESLDRLFLGGGD
                     SSFWSQLSAQILELGDLAFFESAP"
     gene            6803..6973
                     /locus_tag="PKW60_04190"
     CDS             6803..6973
                     /locus_tag="PKW60_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98660.1"
                     /translation="MKHVTIVSKPAKAEQTAWVDLKNVFGFDLGFIDLSSPLTTGQAH
                     WILDEINNALTK"
     gene            7185..7331
                     /locus_tag="PKW60_04195"
     CDS             7185..7331
                     /locus_tag="PKW60_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98661.1"
                     /translation="MKHLKKVTKPSQAAIWQWPGEVLAVKQGWINAVSDPAPGMNPTK
                     PTEV"
     gene            7431..7616
                     /locus_tag="PKW60_04200"
     CDS             7431..7616
                     /locus_tag="PKW60_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98662.1"
                     /translation="MKHIARVSKATPAKAQTIDLKSLLFEPGILGFNEIGDFATWSAN
                     TVAGWFGFSVAPAKSGA"
     gene            7876..>8374
                     /locus_tag="PKW60_04205"
     CDS             7876..>8374
                     /locus_tag="PKW60_04205"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015210041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPJ98663.1"
                     /translation="MENFDYFNPTRIVFGKGTIARVAALAPKDTPILMTYGGGSIKNN
                     GVYAQTKTALQGFDVKEFGGIEPNPRYETLMQAVELARRENIGFLLSVGGGSVLDGTK
                     FIAAAIRFKGSDPWDILAKRAKVDDAIPLGAILTLPATGSEANGTAVVSRESIREKRA
                     FFSEKV"
BASE COUNT         1635 a         2405 c         2717 g         1617 t
ORIGIN      
        1 cgagcacagc agttccgttc actgtatcgg cccacgcgag cgaagcggaa ctggccgcgg
       61 atgcgctggc ccacagctac gccgggcgct ttgggcaggt gcttgagccg atcacgcagt
      121 atgcggggtt tgactggcgc accaacatcg cgctggtcgg cggtttcatg gcgaaagaag
      181 tggttgtggg caccctcggg accgttatgg ccatgggcga cgtagatccc ggccaggccc
      241 aggcgctttc ccagcgtctg gccgccgatc ccgcgtggaa tcccctcaaa ggatttgccc
      301 tgatggtctt tgtgatggtg tatgcgccgt gtttcgtgac catggccatg atccggcggg
      361 agagcggctc gtggaaatgg gcgctcttct ccacgggtta ttctacaatg atcgcattag
      421 tgttggcagt gctgatttac cagggaggcc ttgcgttagg cctcgggata tcctgagaca
      481 acgaacggcg gaccggtaac gctgcggcat gcgagcccgg gtccctgaaa cgggtacgag
      541 ctactgtcca gtggccggtt ttccgaagga gatagcgcaa tgctggaatt gcttgtggtt
      601 ggcttgatcg tgtgcgccgc ggcgtttttc gctgcacggt ggcttcgcag aagtacacag
      661 gggcgtaatt gccacgaaat ctgcggtata gacgcgcgtt cctgccctct gtcgagcgaa
      721 tatgtgccaa ctgaggcagg cgcccgcctt ccaaaggcct gctgcgggat acaggtgtca
      781 gaacagcgcg gccgcacggt ggcgcggccc ggcccgggag gagcagtgtg aattatcagg
      841 gcgtgatact cggcgcagtg gcgtttttga cgatcggcct ctttcacccg gtggtgatca
      901 agggcgagta ctacttcgga gcgcgcatct ggcccgtttt tctggtgctg ggccttttat
      961 gcgtggctct ctcgctcctg gtggataaca cgtacggttc cgtgattctc ggagtcgtgg
     1021 gcttctcttc gctgtggacc atccacgaga tcaaggagca ggaggagcgc gtcgcccggg
     1081 ggtggtttcc caagaacccg aatcggtcaa aataatccgc cacgaatcca aacgtgaacg
     1141 cgcctgcgcg cctggcggcg ctgtcgcggt caacaccaag cgcataatgc actgcgcagc
     1201 ctgccgtcat ccttcagttg ccttgcgaag ctgccgcaag acaggtatcc tgcctctggc
     1261 aacacaacca gcagggaaag gatgggtgca atgcgaggtg ctgtattttt tcagattgct
     1321 ttcttcgtgg cgatggggct gtgcttcggg gcatgggcgg cggaagacac gccggtgaac
     1381 ccgtttacat tgccgggttc gtggtataag gccaaccttc acacacacac cacggtgtcg
     1441 gatggcgacg tggatctccc cgtgcgcgtc gagcaataca aatcgcatgg gtacgacatc
     1501 ctcgcggtga gcgaccacga gaagaccagt gatgtcgcgc cattctgtac cgaagatttc
     1561 ctcctcatca gcggcatgga aacgcatccg aaatcgccga atccctcgat ggcctaccat
     1621 ctcttgtgca tcaacgtccc cctgggcctg tcgtttgaga aagagacgcc catggaggag
     1681 cgtttgaagg tattgcgtga agccggagtc ttcgttgtgg ccgcacatcc gtactggtgc
     1741 gggttcactc tcgatgaact ccttccactg agagaacatg gcgtcgccgc gatggaggtg
     1801 tacaactcaa cgtgccgatt caacgggcgg gctgacagtt cggtgcactg ggaccacctg
     1861 ctgtcccgtg gctggcggat ccccgccatc gccgtggatg atgtgcacag cgacaattcc
     1921 ctgcgcatgg gatggactat gatcaaggcc gaatctctga cgctcgatgc gatcatggcg
     1981 gcgttgcgcg cgggcgcgtt ctatgcgtcg acggggcctg agttcacaga tatgcgagtc
     2041 gaaggcgaca gcgtggtcgt ggagtgcacc cccgtgcaga agatccaatt ttacgcgcct
     2101 gcgcccaacg gctcacagat cctcgcgagc acggagccgc ttacgaaagg cgtgttcacc
     2161 atgaaacgta agctggccta tgtgcgcgct acgatcaccg atgccaacgg caaagaggcc
     2221 tggtcgccgg tcctgtattt cgattagcaa gccgccggcg ctgaactggg ccggcgttat
     2281 ggcgtttcgc gcaggcagtc gaagacacgc tcttcgccgc cgcggtacag caggttgtcg
     2341 tccacttcga gggtatcctc agcggacagg gggatgaaac ggccctcggc ccgggcgtat
     2401 tccgttcctt catcgtccat gatccagctg cgcgcggtgc tgacccgccg gttggctttg
     2461 acgcactccg cgcaggcgat cagcgggcga tttgcgggga cgggtttgag gtaccgcacg
     2521 gtaagctcgc ccgtgaaaca catgcggtgg atcttgcggg cgacggccca gcccatgcac
     2581 tcgtccagca gcgcggcgac gatcccgccg tgcatcacgt cggcgaatcc acaatggtgt
     2641 tttttgcccg tgaacgttgc cttgacgatg tcggcgtcga cataaaagcg cgtctggacg
     2701 cctgcggcgt tctcttctcc acagatgaaa cagggctttg aattcggcaa ataggttatt
     2761 ccgtcactgc ccacatccat tcctttcatt gcccggcgcg ccgccgttgc ggccgcaggc
     2821 ggtgccgcgc ccgtgtccgg caagggccgc aagcgcgtag cggtggccgg ccagccaccg
     2881 gtaggctgca ggcgacgcgt ggcggatgcc cgggaaccgc aaaatacggg ccatccagtg
     2941 ccagccggcc accaagtcca gcaactgggg cagcgcctgc tcaccagagt aaacgcgacc
     3001 gtccgccgtg accagctgta tggcggcaag acactgttca cgggtgaccg ccgggaaacg
     3061 ccgcgcccgg tcttcgtgct ggcacgggag cagctccagt gcgccgcgcc gggcatgctt
     3121 cccggcccac gcggcggcac ggcagcacag cggacaatcc ccgtcgtaaa tgagcgttgc
     3181 ctggctcatt gtgccgctat cctacgtggt gagcccgcca gggctcaact tttcggctgt
     3241 ttgcagaatt gcaataccgg ctccggatgg ttaccctttt gtggcaacac tgctggagaa
     3301 gggccgactc atgcaacttg aatttccatg gtacagcaca tgccagcttc gagaaacgct
     3361 ggaacagcgc tcggggttac ggctcgagct gaccctgacc gacaatgtcc acaacctgct
     3421 ctcggtccgg ccgagcggcg cccgcgggca ggtcaaggtg cgtctccacc acctcttcct
     3481 cgccgcggat gattccgtgc tggacgcatt ggcgacgtgg atcagccgcc cggaagccaa
     3541 gcgggccggg accctgctgg atgactttat tcaggaaaac caccacaaaa tccggcaggc
     3601 gccagaccaa gtccggatca tccgcatccg gacgcgggga cagtgtttca acctcaagaa
     3661 gctgtttgac gaggtaaatc agcgccattt cagcgggacc atcaaggcag ccatcacctg
     3721 gggccggatg cccacggtgc ggccgcagcg ctcgatacgg ttcggcagtt acagctctga
     3781 attcaagctg atacgcatcc acccctttct ggacaggccc tttgtacccg actttttcgt
     3841 ccggtacatc gtttttcacg aaatgctgca tgcccatctc ggcgttgttg aaaccgaaga
     3901 tggccgacac ctggcccaca cgcctgagtt ccgggcgctt gaacgggcct atcccgacta
     3961 cgatcgggcc gtggcgtggc agaaaaatcg aagtacgatg cgccggcttt tccggtgagc
     4021 ccaatatcct gcgccgccga atctgccgaa cccgatagag tgtataatgg gccgcgacat
     4081 tgtcccatat tttgtggttg cggccgctcg gggtatacta tagatggagt tcggaggagc
     4141 acctggcttt tacgcccgcc cgcgggaggc ccggaaaccg gctcgagcgg atgcgccaca
     4201 tgagcctgag cgatgccccc cgcgggagaa gcgcgggtcc cgtgagcctc tcataatggg
     4261 gatgcctgac aacaagtaca gggactgtcc ggcgtgaccg gtgaattgat aagaggcagc
     4321 gcggcaggtt cgaggcaaag cgagagcgtt gtgacgttta gcatggcgga ttgtaacatg
     4381 aatgggtggc gagatgagta gcgctgcgtt caggggcgct gacttgcccg ccaaccgggc
     4441 acactgcgtg gacgaggtcc agaagacgga cgaagaactg atggttgccc tccgcgaggg
     4501 cgaccgggag gccctggccg agctggtacg gaggtatcaa cgggatattt tccggttttg
     4561 ccagcattat cttcgggact tcgagctggc ccgcgagctg acgcaggaga cttttgttcg
     4621 ggtctacgtg gcgcgccagc gcttcgatga ggcccgcaag ttccggccgt gggtgctgtg
     4681 tatcgcccgt aatctcagcc tgaacgagtt gaaacgcaag aagcccgtgc gcatggagtt
     4741 gcttgaggaa tatgccagcg tggcccgcgg cccggacggc gaactgcacc ggtcagcgga
     4801 tggcataccc gaccaggaac tcatggaaaa ggaacgccgg gatgccctgg cgcggctgct
     4861 ggattcactc gacgccgagt cccgggagat cgtgatgctg cgctttttcc agaaaatggc
     4921 cgcaaaagac attgcggagg tggtcggcag caccgaaggc gccgtgcgta cgcggattca
     4981 tcgtatttta aaatcgcttc gtgagaagag tctggcgacg aaggatcatt tgtgagagac
     5041 cgtgagtatg acataagcct cgacgtcctg gccgacggtc tggaggggcg gctttccgaa
     5101 gcgcagctgg cggagctgcg ggaagcgcgg gcagcgtcgg ccgaggttcg ggccaactac
     5161 ctgtggttgc agcgggcagc ccacgatctc gaactcatcg gtgaagaatc cgtcaaggag
     5221 gccccggaaa ttgatgtcgt ggaggcagtc ctgcgcgcgg tagacaaggc ggccgcctcg
     5281 gagaagacgg cccgcctggc ggcgcgggag ccgcagcgcc cgtcctttgt attctggggc
     5341 agggcagcgc tggcggcagc cgttctggcg atggtctttg ggatggtgct cgaattgggc
     5401 ggtttcatgc cgcctgagcc tgcggcgccg gcagtttctc aagtgcctga gctcccggtc
     5461 accccggtcg aacaaatggc gacggcgctt ccgggcgagg tatcaggttc tgaacgcctg
     5521 gacgatgccg tggagcggct ggcgcaagag attactgaaa agctggacac ctcctcgaag
     5581 cacgccggcc ggggcgattt tctcgccacg gccgcaccgg tgctgagcag tgtgacgcag
     5641 aacgacgtgg tggccctgcg ccaggcggcg atctccgaac cgggcgcgtg ggtgcgcctg
     5701 aaacgcatgg cgacgctgga tgccgcaacc gcagcagagg tgctcgagaa tcccgatctg
     5761 ccgccggacg ccatggtcgg cgttgcggcc tcgctgccgg acgaaatggc ccgccaatgg
     5821 ctggtgaccg cggtggggcg gatggatcag caaaaaccgt acgcgaggct ggcccttgcg
     5881 accacctatg tgacacacgc cgcgcctacg gcggacctgc cggcgccggc gctgtttgat
     5941 gggatgagtg aaagtgattt gcgggcgttt ctggctgaaa tggcagcact ccaggaagct
     6001 gaccccgaaa atgccctgct ggatgcattg caggcgggcg ccctgctgcg cctgggtgat
     6061 accgaagggg cgctcgccgc gctcgcgaac ctgcagggca aaggagtggc caccgcctat
     6121 ggactcgacg ccgcacggtc gcggcaactg gcgctggccg cagccggcat gccccaggaa
     6181 tccgcgcgca tgttgagtgc ggtgctcgca ggctcggatc aatataatat gctttgcgat
     6241 ctcgcacgcg atctggtaca atccggtcaa gcctatgagg ctcaaggtga tccggatacc
     6301 gcgcagcgga tttatgaagc cacccagcgc ttgggggagc aagtgacggg aaacacccgg
     6361 ctcagcgagg agtctctcgc ggggattgac atccagcgga tggccatgga aggcttggaa
     6421 gccttgtacg ccaaccgcgg cctcgcgagc gaaatggagc gattgacgtc ctctgctctc
     6481 gcgctcgtca gccagattaa caacctgggg ggaatgctgg aatcattgga tcggttattc
     6541 cttggcggag gcgatagcag cttctggagc cagctctccg cgcagattct tgagctcgga
     6601 gacttggctt tttttgaaag tgctccatga tgacgggagg gccatacggg aacgaatgta
     6661 aaaaggggtt cccttatttc gaaaaaacct gtagaatagc gccgacggtt ccaccgaagc
     6721 ggtggatttt gttggggata agcccggaaa ttctgggtgg acgccaaagt accgaggaag
     6781 caggaaggag gtgaaacgcc acatgaagca cgtcacgata gtgtcgaagc ctgccaaggc
     6841 cgagcagacc gcatgggtcg acctgaagaa cgtttttggg tttgatctgg ggtttatcga
     6901 cttgtcgagc cccctgacga cgggtcaagc ccactggatt ctggacgaga tcaacaacgc
     6961 tctgacgaag taagtgcacg agcgtgaagc ccaatcacgg ggtcgtgtgc gaaacagcgg
     7021 aaggagagtg ggcgtacgtg gttttcacct tggtcagtcc actccaagag tttccgtgaa
     7081 gcggcggccc gctgtgggcg tctgatgcag ggtatgtgag ctttcgagcc cgcgtccggc
     7141 acggctgcgt acggcgggga aacgctcaag aaaggtatgg agaaatgaaa catctgaaaa
     7201 aggtcaccaa gccgtctcag gcggcgatct ggcagtggcc gggagaggtc ctggcggtga
     7261 agcagggctg gatcaatgcg gtcagcgatc ctgcgccggg aatgaacccg acgaaaccca
     7321 ccgaagtgta ataaactcat gtcgtggcgg ggcgccaggc gccccgcagc tgtgagcctg
     7381 gtttggtttg cgccacgaag cgcgcagact gtggagggag gttaaacccc atgaagcata
     7441 tcgctcgcgt ttcgaaagcg acgccggcaa aagcgcagac gattgatctc aaatcgctgc
     7501 tgttcgagcc gggtatcctt ggtttcaacg aaattggtga tttcgccacg tggagtgcga
     7561 acaccgttgc cggctggttc ggcttctcgg ttgccccggc caagtccggt gcgtgagtct
     7621 cgaagcgctt ttttaagctg acttcgaaaa gcccagcttg tcatacggct ttgcgtccgc
     7681 gagccggaac cgaggtatag tgttccggct cgtgggcgtt ttttgtctct accagccaca
     7741 ggacgcgccc accgcagccc ggatttttcc cgcgggtccc attgtaccca ttcgttcgtg
     7801 ttaggattaa gagaacatga gcaaacggga acgaggcggc tggatactgc tgcctgacta
     7861 ttcggaggtg aggccgtgga gaattttgac tatttcaacc ctacgcgcat tgtctttggc
     7921 aaaggcacga tcgcgcgcgt ggccgcgctg gccccgaagg acacgccgat tctgatgacc
     7981 tacggcggcg ggtcgatcaa gaacaacggg gtgtacgcac agacgaagac ggcactccag
     8041 gggtttgatg tgaaggaatt cggagggatt gagcccaatc cccgctacga aacgctgatg
     8101 caggccgtgg aactggcccg ccgcgagaac atcggctttc tactgtcggt cggcggcggc
     8161 tcggtgctgg acggcacgaa attcattgcg gcagcgatac gctttaaggg cagcgacccc
     8221 tgggacatcc tcgccaaacg agcgaaggtt gacgacgcga tcccgcttgg cgccatcctc
     8281 acgctcccgg caaccggatc tgaagcgaac ggcaccgcgg tcgtgtcgcg cgagtcgatc
     8341 cgcgaaaaac gggccttctt cagcgaaaag gtct
//
LOCUS       DAOPBQ010000085         8326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7947_length_8326_cov_4.423044, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000085 DAOPBQ010000000
VERSION     DAOPBQ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7947_length_8326_cov_4.423044"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..249
                     /locus_tag="PKW60_04210"
     CDS             16..249
                     /locus_tag="PKW60_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98664.1"
                     /translation="MFAFLGSYNSLMWPLVVTDRDEMRMVMVGLTLFSSDEGVRLHLL
                     MCAATVVILPTLGLYFVAQRYFLESAISAGIKG"
     gene            246..1535
                     /locus_tag="PKW60_04215"
     CDS             246..1535
                     /locus_tag="PKW60_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPJ98665.1"
                     /translation="MNIRSAVSVAVLAMGMAAFLPACGGSGEFRPIDPNTALFWDRQT
                     DENRELLENIISEFNAGHGGIPVKADYVGGYSEIFRKLVTSIQARTVPAMAVGYQSMT
                     TVYAQAGAIVPLDPLLADPEKGVSEQELADFFPVVLETNRYPTLENGMYSFPFCKSVL
                     MLYFNKRVLAAAGIGEPPHTWDEFLSQCRQIKERTGKKAYAVAVDASTFDGMVYSMGG
                     EVYANGASCFDSPETLAVLKLHETLGQEELAYQILAHRYDDRADFAQDKTAFFFRSSS
                     HRTYTAMLIEPDAWGMTVIPQADPENPKTVLYGPNINIFRTTPEQEQVAWEFAKYFTS
                     PETTVRWALGTGYLPIRKSAAQDARMQAFWEEWPYNKAPFDCLSFARSEPNVPGWQEV
                     REHIENALAGVLTGVKTAEQAAAELKRNADSVLAESR"
     gene            complement(1613..1750)
                     /locus_tag="PKW60_04220"
     CDS             complement(1613..1750)
                     /locus_tag="PKW60_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98666.1"
                     /translation="MSFDHPDRRRSSRRQADLELAEREHKEMIEEALEEFAAEHDPDH
                     P"
     gene            complement(1979..2776)
                     /locus_tag="PKW60_04225"
     CDS             complement(1979..2776)
                     /locus_tag="PKW60_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPJ98667.1"
                     /translation="MREHIIQRLKSLAIACLALCLAACGPRGLRSNADAPLPLTLTTE
                     DGRTLPAVFYPAGTKPACGVVLVHDKGGAGSQWAAFATRLQQSGIASLAFDMRGHGAG
                     TEESLPYRDFSPSDWLGAGFDIQAAVHALITHGVDPDNIGIVGAGMGATLATHYAAQD
                     ETIQALVLISPALEYDGLSIRRAFEAYIRRPSLLVAAKGDTYASSSARALKKTASTFC
                     ELREYDGGAHGIDALDSLSGAREEVAYWLESILKTSRHPVERKHRAE"
     gene            complement(2822..3148)
                     /locus_tag="PKW60_04230"
     CDS             complement(2822..3148)
                     /locus_tag="PKW60_04230"
                     /inference="COORDINATES: protein motif:HMM:NF026214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HPJ98668.1"
                     /translation="MADTVAGFFSELKAMVDPEKATGMNATYQFDITGDGGGAWHVTF
                     AGGSVVVTEGKAAAPNITLTSDATTWMDIVNGRTSGQTAFLLGKLKIQGDMSLAMKLA
                     SVFGFG"
     gene            3969..4250
                     /locus_tag="PKW60_04235"
     CDS             3969..4250
                     /locus_tag="PKW60_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98669.1"
                     /translation="MAPAKKTAAKKKAVAKKKPAAKKKAVAKKKAVAKKKAVAKKKVA
                     AKKPAAKKKVAAKKKPAAKKKVAAKKPAAKKKAVAKKKPAAKKKAAAKK"
     gene            4505..6691
                     /locus_tag="PKW60_04240"
     CDS             4505..6691
                     /locus_tag="PKW60_04240"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPJ98670.1"
                     /translation="MEEIFRQALDAAVRTSGSPGAVAYVGNRDSTLFVEAIGNAQLEP
                     VPAPAKLNTIYDLASLTKVVATTTALLILRGQKAVDFERPVSNYLPMPAFRAITLRHL
                     LTHTGGLVAGLPLYRTCSSMDAMLEEYGKEGIQNAPGISWRYSDAGFMLLGRIVEQVA
                     GKPLDRFCTEQIFAPLKMTDTFFNPPAKLRPRCAATERCTWRNRLIQGEVHDENSYAV
                     GGVSGHAGLFSTAEDLARFCRAFLGGELVSQAVLDEVAAVGQVPSYPWQGLGWWLDPW
                     DTATTGFLPSRQALGHTGWTGTSLWMDRQTGIFAILLANTCHPVRDSRNNGVLRNTFY
                     RAVARALYPRRTNTHAGLDRLLRENFQVMRASKRAALLTNHAAVDQMGRHILDVFNLG
                     ASSRLHVLYSPEHGIRGTHEAGASVASERAAVPVISLYGKQTRPSAAELAEVDYFLID
                     LQDIGSRYYTYMATMRECLAACAQAKKPVLILDRPNPLGGVVLEGLIAERTGSPVCCA
                     PIPVRHGMTMGELAIFFLREEFSRQKLNLSVELLDSWRRDALFDECAYPWVPPSPNIP
                     TPLTALLYVGTCLFEGTNLNEGRGTATPFHVFGAPWLDAESVIGRIEPHEMPGCRLTA
                     CRYAPRAIPGKASTPRYLDEECNGIRIQVNRPLEVRAFTLVLALLRAIRKQHPRHFEW
                     KAFFDVLAGGEKLRTWLEQDRPAADIAAQYASQLDAFQASRPRRYE"
     gene            complement(6737..6934)
                     /locus_tag="PKW60_04245"
     CDS             complement(6737..6934)
                     /locus_tag="PKW60_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98671.1"
                     /translation="MIFVRTFKGYEDKTASLDAEVNEWIERHALDIVDIKTVLGHENG
                     SRAGSGDVLYTVLYRADAPLD"
     gene            complement(7062..7895)
                     /locus_tag="PKW60_04250"
     CDS             complement(7062..7895)
                     /locus_tag="PKW60_04250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPJ98672.1"
                     /translation="MRPRILVVHTGGIGDFLLCCPSIQRMARDAAVELLGRPERLALA
                     LDAGLVERIHDLEAAGFDSLFATPSAAIRALLSRFDAAVVWMRDDDGALKRAIQSCGL
                     TDVRTFPGLPPQSWARHAAEYYLHCLGFETSALFRLTLPSVDAPLDVVIHPGSGGKAK
                     NWPFERFLRLSEALRARGREAHWCVGPAEEGISLPKDAKVVCEASLAALARRLAAARD
                     YVGNDSGITHLAAACGTRTTAVFGVTDPRVWAPRGEEVQVVSGTPWPGVDAVLSVLLR
                     E"
     gene            complement(7955..>8326)
                     /locus_tag="PKW60_04255"
     CDS             complement(7955..>8326)
                     /locus_tag="PKW60_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPJ98673.1"
                     /translation="LDTFDALFDAIPDDHFGLNYDPSHLYHMQCDHLAPIAMFSDRIF
                     HTHAKDTLVDKIARSYVGVYGSGWWRYVIPGFGNINWGEYISHLRHAGYDGVLSIEHE
                     DETQSVEEGFMRGAAFLEQFC"
BASE COUNT         1724 a         2531 c         2505 g         1566 t
ORIGIN      
        1 gccgtcacgg tgtgattgtt cgcgtttctg ggcagctaca actccctgat gtggccgctg
       61 gtggtaacgg accgggacga aatgcggatg gtgatggtcg gcctgaccct gttttcctcg
      121 gatgagggcg tgcggctgca cctcctgatg tgtgcggcga ccgttgtgat ccttccaacc
      181 ctgggactat acttcgtagc acaacggtat ttcctcgaaa gcgcgatcag cgcgggaatc
      241 aagggatgaa catcaggtca gcggtgtcag tggccgtgct ggccatggga atggccgcgt
      301 tcctgccggc ctgcggcggc agcggcgaat tccggcctat cgatccgaac accgccttgt
      361 tctgggatcg tcagaccgac gaaaaccggg aattgctgga aaacatcatc tcggaattca
      421 acgcgggcca cggcggcatc cccgtcaaag ccgattacgt aggcggttac agcgagattt
      481 tccgcaaact tgtgaccagc atccaggccc gcaccgttcc cgccatggcg gtagggtacc
      541 agagcatgac cacggtatat gctcaggccg gggccatcgt gccgctcgat ccactgctcg
      601 ccgaccccga gaagggagtg agcgaacagg agctggcgga tttctttccg gtggtgctcg
      661 aaaccaaccg gtatcccacg ctggagaacg gcatgtattc gttcccgttc tgcaaatcgg
      721 tcctcatgct ctatttcaac aagcgcgtgc tggccgcggc gggcatcggt gaacctccgc
      781 acacctggga cgagttcttg tcacagtgcc gccaaatcaa agaacgaacc ggcaagaagg
      841 cgtatgccgt cgcagtagat gcctccacct tcgacgggat ggtgtacagc atggggggcg
      901 aggtgtacgc caacggcgca agctgcttcg attcgccgga gacactggcg gtgctgaagc
      961 tgcacgaaac gctcggccag gaagagctgg cctatcagat tctggcccac cgctacgatg
     1021 accgcgcgga ctttgcacag gacaagaccg cctttttctt ccgttcaagc tctcaccgca
     1081 cgtacacggc gatgctgatt gaaccggacg cctggggaat gacggtcatc ccacaggccg
     1141 atccggagaa tccgaagacc gtgttgtatg gtcccaacat caacatcttc cggaccacac
     1201 ccgagcagga gcaggtcgcc tgggaattcg cgaaatattt cacgtcgccc gagaccaccg
     1261 tgcgctgggc acttggaacc ggctatttgc cgatccgcaa gtcggcggcc caggacgctc
     1321 gcatgcaggc gttctgggaa gaatggccct acaacaaagc ccccttcgac tgcctctcgt
     1381 ttgctcgttc cgagcccaat gtacccggat ggcaggaggt gcgggagcac attgaaaacg
     1441 cgctcgcggg cgtattgacg ggcgtcaaaa cggccgagca ggccgcggcg gaactgaaac
     1501 gaaacgccga cagtgtgctc gccgagtcgc gttaagcgaa atctagcggc cccgggcggc
     1561 cttctccccc agcgcctttg cggcacgccg gccggtgttt gatctccacg ggctacgggt
     1621 ggtccgggtc gtgttcggcc gcaaattctt cgagtgcttc ctcgatcatc tctttgtgct
     1681 cacgttccgc gagctccaga tcggcctggc ggcgtgagct gcggcgccgg tccggatgat
     1741 caaaggacat gttgcgtttt cggcggtcat ggcttgcacg acgctctttc ttccactccc
     1801 ccgaatggtc ctggaaaaag cctggatggt gccctgtgtc tgacacgtca ctaaccccgt
     1861 gattgcaact ttccattcca tggcacatat gccgggacgc agtataggag gcgtctccgc
     1921 cagcgtcaag caggttttgt gaaccgagct gcgcttcact gccctgcaat atgcgggcct
     1981 attcggcgcg gtgtttccgt tcaacgggat ggcgcgatgt tttcaggatg ctctcgagcc
     2041 agtacgccac ctcttcccgc gctccagaca gagaatccag cgcatcgatc ccgtgtgccc
     2101 cgccgtcata ttcgcgcaat tcgcaaaacg ttgatgcggt cttcttgagg gcgcgcgcgg
     2161 aggaggatgc gtatgtatct cctttggcag cgaccaggag cgaagggcgc cgtatatagg
     2221 cttcaaacgc cctgcggatg gaaaggccgt cgtattcaag cgcgggagag atcaagacaa
     2281 gggcttgaat ggtttcgtcc tgcgcggcgt aatgggtggc cagggtggcg cccattccgg
     2341 cgccaacgat cccgatgttg tcgggatcga caccatgtgt gataagtgcg tggaccgccg
     2401 cctgaatgtc gaaaccggcg cccagccagt cggacggtga gaagtcgcgg tagggcaggc
     2461 tctcctccgt gccggcgcca tgtccccgca tgtcgaaggc aagggatgcg atgccgcttt
     2521 gctgcaggcg tgtcgcgaat gcggcccatt gcgaaccggc tccgcctttg tcgtgaacca
     2581 gcacaacgcc gcaggcgggt ttcgtgccgg cggggtaaaa cacggccggc aaggtacgtc
     2641 catcttccgt tgtcagcgtc aggggaagcg gagcatcggc attggaccgg agcccgcgcg
     2701 ggccgcaggc tgcaaggcag agtgcgaggc atgcaatggc cagagacttc agccgttgga
     2761 taatgtgttc acgcacggga tgcaccggaa atgcgccgaa gcgggaacgg cgccctgtac
     2821 ttcatccaaa accaaacacg ctggcgagct tcatcgcgag actcatgtcg ccctggattt
     2881 tcagcttgcc gaggagaaaa gcagtctgcc cgcttgtgcg tccgttgaca atatccatcc
     2941 atgtcgtggc atccgaggtc aacgtaatgt ttggcgcggc ggccttccct tcggtgacca
     3001 caacactgcc gccggcaaac gtcacatgcc acgcaccgcc cccgtcaccg gtgatatcga
     3061 attgataagt agcgttcatg cccgttgctt tttcggggtc caccatcgct ttgagctccg
     3121 aaaaaaaacc tgcgactgtg tcggccatgt ctctaatccc tttcgtgaat agttgcgttt
     3181 tataccacca ttacaggctg aaatggctgt cgtcgggcgc gttattcact gcattcgcat
     3241 gcgcgttcac gctcatgaac gcatcggcca ccgggcgttg gttactctaa cacaggcctg
     3301 cgacaaatca aagagaagga aaaaatgatc gtttctcgat aaaaacactt gctatttttt
     3361 gaactgtctg ctacacattt atatggatag catgagtaca cgctaaagag aagacagttg
     3421 aagccagaga caagacgacg cgcggcacga agcgcgatgc gttttcgtcc cgataaaata
     3481 aggccaaact cgcttccaca gcacgcgggg cctattgcgg gcgagatgga aaatcgaggc
     3541 tactcgcgaa caatttgaac acgctgattg cgcagatcgc tcgactgaac gagcactcat
     3601 ccgtctcagg gggacataaa gcagtagacg gatcctcagg ggaagggaat cgacgacact
     3661 tgagttgggg gtcagacaca ccgcgactgc actgcgactc acaagcacag tcaccagcgt
     3721 attgcgtgca tgcaaggccg tctttcgcag cacgtgcccg acgaacgaaa cccaagaatg
     3781 tccgcgcgcg actccgggga cattgcgggc aggcgacaag gccacaccga gcctccggct
     3841 cgtttggcat agggctacat gaccacgtca tgagccgtgt tgacggaatc tgcgccatgg
     3901 cattgctggc atggccggct actgtcaaca cacgtttcgg ggtttagttg cgaaaaagga
     3961 ggaacaccat ggccccagcg aagaagaccg cagccaagaa gaaggccgtc gccaagaaga
     4021 agccggccgc caagaagaag gccgtcgcca agaagaaggc cgtcgccaag aagaaggccg
     4081 tcgccaagaa gaaggtcgcc gccaagaagc cggccgccaa gaagaaggtc gccgccaaga
     4141 aaaagccggc cgccaagaag aaggtcgccg ctaagaaacc ggccgcaaag aagaaggccg
     4201 tcgccaagaa gaagccggcc gccaagaaga aggcagcggc gaaaaagtaa ccgtagccgt
     4261 ctcttcggca tttggccgga ggcatctgct ctttgaaacg gaaccaccgt tttcaagggc
     4321 agaggcctcc ggtctctttt ttttggcgct ccacccgcga cctcaacgca ttctggtggc
     4381 cgattcggcc gtgtcacttg cgccctgcat tctgaatcac tagactgcag gctcagcatt
     4441 gcttcaagag tatctgtcac tatctggccg gcgctttgtc agcctggtgt gagggagata
     4501 tcgcttggaa gagatctttc gccaagctct cgatgcagcc gtccgcactt ccggaagtcc
     4561 gggggcggtg gcgtatgtcg gcaaccggga ttcaacgtta ttcgttgagg ccattgggaa
     4621 cgcccagctg gaacctgtcc cggcgcccgc gaaactcaac accatctatg atctggcgtc
     4681 actgaccaaa gtcgtcgcaa ccaccaccgc actgctgata ctgcgcggtc agaaagcagt
     4741 tgatttcgag cgtcctgtgt ccaactacct gcccatgccc gcatttcgcg caatcacgct
     4801 gcgccatttg ctcacccata ccggaggatt ggtcgccggg ctgccgctct accgcacctg
     4861 ctccagcatg gacgccatgc tcgaggagta cggaaaagaa ggcatccaga acgcgcccgg
     4921 catttcctgg cgctattccg atgcgggctt catgctgctg ggccgcatag tggagcaggt
     4981 ggccggcaaa ccgctggatc gcttctgcac ggaacagata ttcgcccccc tgaagatgac
     5041 ggacaccttc ttcaatccgc ccgcgaaact gcggccgcgc tgtgcggcca ccgaacggtg
     5101 cacgtggcgc aaccgcctca tacagggcga ggtgcacgac gaaaacagct atgcggtggg
     5161 tggcgtgtcg ggacatgcgg gcctcttctc gacggcagaa gatctggccc gcttctgccg
     5221 ggcgtttctg ggaggcgagc tggtatctca agccgttctg gacgaagtgg ctgcggtggg
     5281 gcaagtcccc tcgtacccat ggcagggact gggctggtgg ctcgacccct gggacaccgc
     5341 gacgacgggg tttcttccgt cacggcaggc gttagggcat accggctgga ccggtaccag
     5401 cctctggatg gatcgccaga ccgggatctt tgcgatcctg ctggccaaca cctgtcatcc
     5461 cgttcgggac tcgcgcaaca acggcgtgtt acgcaacaca ttctaccggg cagtagcccg
     5521 cgccctctat cctcgccgca ccaatactca tgcggggctt gaccgcttgc tgcgagaaaa
     5581 ttttcaagtg atgcgcgcct cgaagcgggc tgcgctcctt accaatcacg ccgcggtcga
     5641 ccaaatgggc cggcacattc tggacgtctt caatctgggc gcatcgtcca ggctgcatgt
     5701 gctctacagt ccggagcacg gcatccgggg cacccacgag gcgggagcca gtgtggcgtc
     5761 tgagcgcgcc gcggtgcccg tgatcagcct gtatggcaaa cagacgcgcc cgtccgccgc
     5821 ggagcttgcc gaagtcgact attttctgat tgatctgcaa gacatcgggt cgcggtatta
     5881 cacctacatg gcgacaatgc gggaatgcct ggcggcatgc gcgcaggcaa agaaacccgt
     5941 gctcatcctg gaccgtccga atccactggg gggcgtggtg cttgaaggac tcatcgccga
     6001 acgcacgggc tcgcccgtgt gctgtgcgcc gatccccgtt cgacacggga tgacgatggg
     6061 tgaactggcg atatttttcc tgcgcgaaga attcagcaga caaaaactga acctctccgt
     6121 ggaattgctc gacagctggc ggcgggatgc gctgttcgac gagtgcgcgt atccctgggt
     6181 gccgccatcg ccgaacatcc caacgccgtt gacggccctt ctgtacgtgg gcacgtgcct
     6241 gttcgagggg accaacctga atgaagggcg cggcaccgcg accccgtttc atgtgttcgg
     6301 cgcgccgtgg ctcgacgcag agtccgtcat cggtcggata gagccgcacg agatgcccgg
     6361 atgccgcctg acggcgtgcc ggtatgctcc gcgcgcgatt ccaggcaagg cgtccacccc
     6421 ccgctacctg gacgaggaat gcaacgggat tcgcatccag gtgaaccggc ctctcgaagt
     6481 ccgggcattc accctggttc tggcgctgct gcgcgccatc cgaaaacaac atccccggca
     6541 cttcgagtgg aaggccttct ttgacgtgct ggccggcgga gagaaattac gtacctggct
     6601 cgagcaggat cggcccgcag ccgacatcgc cgcccagtac gcgtcacaat tggatgcttt
     6661 ccaagcctcc cggcccaggc gctatgagta ggcgtgaaga cgcgcagcga ttgaaacaag
     6721 atccgtagtc tgctgcctag tccagcggcg catcggcgcg atacagcacc gtgtagagga
     6781 catcgccgga acccgcccgc gacccatttt catggccgag aacggtcttg atatcgacga
     6841 tatcgagcgc gtggcgctcg atccactcat tgacctccgc gtcgagagag gccgtcttat
     6901 cttcgtatcc cttaaatgtc ctgacaaaaa tcatggcgcg acctcctgcg cgttctgggc
     6961 ggttttccgc acccagtgta tcacgaaatt ctggattcgt tgaggcatcg gcgttcaggc
     7021 actctcagaa gccgccagca tggccgctgt gatgcccata gtcactcccg caagagaacc
     7081 gacagcaccg cgtcgacccc gggccatggc gtgccgctca ccacctgcac ctcttcgccg
     7141 cgcggggccc agacccgagg atccgtcact ccaaatacgg ccgtcgtacg tgtcccgcat
     7201 gccgccgcta gatgcgtaat gccgctgtcg ttgccgacat aatcgcgcgc cgccgccaga
     7261 cggcgtgcca gagcggccag ggaagcctca caaacgactt ttgcgtcttt gggcagggag
     7321 atcccttcct cggcgggtcc cacgcaccag tgggcttccc tgccccttgc gcgaagcgct
     7381 tccgacagcc gaaggaagcg ttcaaacggc cagttcttcg ccttgccacc gcttccagga
     7441 tgaatcacca catccagggg cgcgtcgacg gagggcaggg taaggcgaaa tagcgcagat
     7501 gtttcgaaac caaggcaatg gaggtaatat tcagccgcat ggcgcgccca gctctgaggc
     7561 ggcagcccgg ggaaggtgcg cacgtcagta aggccgcagg attgaatggc ccgtttcaac
     7621 gcgccatcgt cgtcgcgcat ccagacaacc gccgcgtcaa atcgcgaaag gagcgcgcgg
     7681 atggctgcgg acggcgtggc gaaaagcgag tcaaaaccgg ccgcttccag gtcatggatg
     7741 cgctccacga gcccagcgtc gagcgccagc gccagccgtt cgggacgtcc gagcagctca
     7801 acggcggcgt cgcgcgccat gcgctggatc gatggacagc agagcaggaa atctccgatt
     7861 ccacccgtat gcacaaccag gatcctcggc cgcaccggcc tgatctcctg ccgggatgcc
     7921 tgtgagcagc cagcaaacac ggggaaaggc ctccctagca gaattgctcg agaaaggccg
     7981 cgccgcgcat gaacccctct tcgacggact gagtttcgtc ctcatgttcg atgctcagca
     8041 cgccatcgta cccggcatgg cggaggtggc tgatgtattc accccagttg atgtttccaa
     8101 aaccgggaat gacgtaacgc caccagcccg aaccatagac gccgacgtag gagcgggcaa
     8161 ttttgtccac gagcgtgtct ttggcgtggg tatggaaaat acggtccgaa aacatcgcga
     8221 taggcgccaa atggtcgcac tgcatgtggt agagatggct ggggtcgtaa ttcaacccga
     8281 aatgatcatc gggaatggcg tcaaacaggg cgtcgaaggt gtccag
//
LOCUS       DAOPBQ010000086         8316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7958_length_8316_cov_3.726546, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000086 DAOPBQ010000000
VERSION     DAOPBQ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7958_length_8316_cov_3.726546"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3885
                     /locus_tag="PKW60_04260"
     CDS             <1..3885
                     /locus_tag="PKW60_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007332850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPJ98674.1"
                     /translation="DWDTGALPRSPVPVDAIPDLGENQQIVFTDWPGRSPQDVEDQIT
                     YPLTAALLGVPEVKTIRSMSAFGFSTIYVIFEEAADFYWSRSRLLEKLNSLPPDTLPD
                     GVQPALGPDATALGQVFWYTLEGRDPQGAPAGGWDLAELRSIQDWLVRYALQSAHGVS
                     EVASAGGFVQEYQIDVDPDAMRAAGVTLSDVFEAVRTSNVDVGARTIEVNKVEYVIRG
                     LGFIKTLEDIETTAIKVVDNTPVRIRDVARVSLGPALRAGALDKGGAEAVGGVVVVRY
                     GANPLEVIKNVKARIAEIAPGLPTKAFIDLSKVSQSAVEAFAAEQGFEAYRGADLNQP
                     AWLAWLRAHDPGEWPEWAVLSQVAIVPFYDRTGLIYETLGTLNAAIVLEILVTIIVVI
                     VMVRHLRSSLLISMLLPLAVLMCFIAMRLFRVDANIVALSGIAIAVGTMVDMGIIICE
                     NILNKLKEAGPDGNRLLAVFEASSEVGSAVLTAVATTVVSFLPVFTMTAAEGKLFRPL
                     AFTKTFALLASVIIAVVLIPPMAHVLMGGRPLSLRARRVLGLLAVAAGIAVLVSASGW
                     AGVALIALGVYSLASGALPRWAAGATSVAANVFAVLLVALLLTVAWNPLGPERGFVRN
                     AAFVVAIIGGLLALFFVFQRSYARLLRWFLDHKAVYLVAALAVVLAGATAWLGFDRVF
                     GFVPAVLGRTGVPQERIRTNRLWSTAIHAFPGFGKEFMPPLDEGSYLYMPTTMPHASL
                     GEALDVLQYQDQAISAIPEIDMVVGKIGRVESPLDPAPVSMIETVIQYKPEYISDASG
                     RRMRFRYDRKRGAFDRDGNGQLIPDPGGRPFRQWRDEIRSPDDIWDEIVRAAEMPGTT
                     TAPKLQPIAARQVMLQSGMRAPMGVKIKGPDLETIETAGLALERLLREVPSVEPDAVI
                     ADRIVGKPYLEIDINREAIARYGISIRQVQDVIEVAIGGQRITTTVEGRERYPVRVRY
                     LRELRDQIETLERILVPSPSGAQIPLVQLAKIRYVRGPDAIKSEDTFLVGYVLFDRKP
                     EYAEVDVVEQCRSFLEEKLASGELHLPPGVSYQFAGTYENQVRSQKTLALVLPLALFI
                     IFMILYFQFRSVPTALLVFSGIALAWSGGFLLMWLYGQPWFLDFSVFGANLRELFQVR
                     EINLSVAIWVGFLALFGIASDDGVVIATFLDQSFAKHRPESIREIRDATVAGAQRRIR
                     PCLMTTATTILALIPVLTSTGRGADIMVPMAIPSFGGMVVELMTIFLVPILYCGVQEF
                     RLRRGRPVLPAALESERAAVAHATPADKEYPS"
     gene            3882..5372
                     /locus_tag="PKW60_04265"
     CDS             3882..5372
                     /locus_tag="PKW60_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPJ98675.1"
                     /translation="MMNLLFVAALAAAGVEAPAFYAGNDELRGLLLEAAENNPTLLAR
                     YETWRAALERIPQVTALDDPSLSYGQFIQSESSRARFAVSQSFPWFGTLRARGDKAAA
                     EADAALQVFFAERNRVFAEVKKAYFEYAFLADAVLVTEAQSEVLEYMEEVVESKLALG
                     LANEDELLRVSMEKAQVRDRRDSYLQNKPARMARLNEVLGRDVLEEIPWPQSTPLPPE
                     LPPAETVVARITEANPDLKALDRLLESREHDIELARKEGYPEFTAGLEYLAISKPRQV
                     GPDRITADNLSTGYRLLNTFTGRMPFEPVNTAIDAYGLATSREPMNYSDGGDDEIMLS
                     FTLSVPVWRKRVKAGIEEARLLRRAAEHDKRGRELALQASAQMAVFEAEDALRRYRLY
                     RDTLVPQAEQTYESLQDQYSAATGGADFLDLLESVQTLLAFELEQVRASRDAQLAASD
                     LEFLMGGPWLLNNGGSEPASEGVSGQQAPYRITVRKSEHNESEEDP"
     gene            5369..6022
                     /locus_tag="PKW60_04270"
     CDS             5369..6022
                     /locus_tag="PKW60_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98676.1"
                     /translation="MTRMIMIMSAAAMALTGAAAHAGEASADAPGSIYTLATCPVTGA
                     ALGSMGAPIVKKYDGREVRFCCAGCVSSFEADKEAYFKKIDAAVINEQKKSYPLDTCL
                     VSGQKLGSMGESVDLVYRNRLVRLCCEGCRAAFQKEPAKYLKKLDAAIVEKQKGAYPV
                     DTCVVSGEKLGAKGDPVDYIAGGRLVRFCCSGCVKEFEKDPRTYLDKLDAAAKPSNG"
     gene            6261..>8316
                     /locus_tag="PKW60_04275"
     CDS             6261..>8316
                     /locus_tag="PKW60_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HPJ98677.1"
                     /translation="MRPVSRLLAALLVCAGCAHVPVNENLTLFQGDPIENVQGVSFDG
                     EVFEVPGAGAISRDEVTELLFQPAEAEGGKAAAPAGVAGLTGLAQEMLPKGRALAEQF
                     PGTGGVILIDDGLFVYNDDGTNRYRYHFAGLVLKEEMKSWAQFAAGFTEGRSRVRVLE
                     AKSVAPDGTIYTLPAEALRVGSPSEEMQFFNPSRKVVGGVIPGVEIGSIVEYIYEYEN
                     YNPEDSRLFLPGYYFQSTEPVALSRVTIEVPDRLPFHHYTRHFPDPALAEPQVERHGR
                     RVHYVWALHDMPPLVPEPMMPPQTDLTPRMEASIFASYEEVFALQRDLQRSRMKLTPQ
                     IEAEVARITEGAASIDEKLARIYHWVQTNTRYISIKGSLGSGLSGHTAMETYENRYGD
                     CTDKAILFATMCEAIGVTSYPIILSTNDNGVGVTEIPTIDGNHAISEVDLPDGRRFYL
                     DSTAQNFRYPYFRADDHGCVAVNAIRGDFNIIPVPPPEDNARHSELEAALAANGDMTV
                     RARNEYTGTIEAGIRAFWKTVRDDERADRMTEYVNSLSPGAILDDFSLSELTELSEPV
                     RMGIDYRLPGHAIRAKKLMYLQMPSLERTFPEVALESRRFPIQYMTTEERTLTIHLAL
                     PAGFRLKWAPPPLRIRTPYLEYDAAYEETGDGVTLKEVFRRLDRIIPVEAYPEYRDGL
                     RAI"
BASE COUNT         1516 a         2667 c         2587 g         1546 t
ORIGIN      
        1 gactgggaca ccggcgcgct tccccgcagc cccgtgcccg tcgacgccat cccggatctt
       61 ggcgaaaacc agcaaattgt gtttaccgac tggccgggac ggtcacctca ggatgttgag
      121 gaccagatca cctacccgct gaccgcggcg ctgctgggtg tgccggaagt caagaccatc
      181 cgcagcatgt cggcgttcgg gttttcgacc atctacgtaa ttttcgagga ggctgccgat
      241 ttttactggt cgcgcagccg cttgcttgag aagctcaaca gccttccccc ggacaccctg
      301 cctgacgggg tccaacctgc gctcggtccc gacgcgacgg cgctcggcca ggtcttctgg
      361 tacacgctcg aagggcgcga cccccagggc gcccctgcgg gcggctggga tcttgccgaa
      421 ctgcgcagca tccaggactg gctggtgcgt tatgcgctgc aatccgctca cggggtcagc
      481 gaggtggcct ctgcgggcgg ttttgtgcag gagtaccaga tcgacgtcga cccggatgcc
      541 atgcgcgccg ctggtgtcac actcagcgat gtatttgagg cggtccgcac atccaacgtg
      601 gatgtggggg cgcgcaccat cgaagtcaac aaagttgaat acgtgatccg cggactcggg
      661 ttcatcaaga cgctcgaaga tattgaaaca acggccatca aagtcgtgga caacaccccc
      721 gtgcgcatca gggacgtcgc gcgggtgtcg ctgggtcctg cgctgcgggc cggcgcgctc
      781 gacaaaggcg gtgcggaggc cgtcggtggc gtggtggtgg tccgctacgg cgccaatccg
      841 cttgaggtga tcaaaaacgt caaggccagg atcgcggaga tcgcgcccgg cctgcccacc
      901 aaagccttta tcgatctgtc gaaagtcagc caaagcgccg tggaagcctt tgcggcggag
      961 caggggtttg aggcgtaccg cggggcagac cttaatcagc ccgcgtggct ggcctggctg
     1021 cgcgcgcatg accccggcga gtggccggaa tgggcggttc tcagccaggt ggcgattgtg
     1081 ccgttctatg accgcaccgg gctgatttat gaaacgctcg gcacgcttaa tgccgcgatt
     1141 gtgctcgaga tcctggtgac gatcatcgtc gtgatcgtta tggtgcgcca tctgcgaagc
     1201 tcgctgctca tcagcatgct tctgccgctg gccgtgctca tgtgcttcat cgctatgcgc
     1261 ctgttccggg tcgacgcgaa cattgtcgcg ctctcgggca tcgccatcgc cgtgggcacc
     1321 atggtcgaca tggggatcat catctgtgag aacattctga acaagttgaa ggaggccggt
     1381 cctgacggca accgcctcct ggccgtgttc gaagcctcga gtgaagtggg cagtgctgtc
     1441 ctgacagcgg ttgccaccac tgtggtcagt tttctgcctg tgttcaccat gacggcggcc
     1501 gaaggcaaac tgttccggcc gctggcattt accaagacgt ttgccttgct cgcatccgtc
     1561 atcatcgcgg tcgtgttgat cccgccgatg gcgcatgtgc tgatgggcgg ccggccgttg
     1621 agcctccgtg cccgccgcgt cctgggattg ttggcggtgg cggcgggcat cgccgtgctc
     1681 gtatctgcgt ccgggtgggc cggcgtcgcg ctcatcgccc tgggtgtata ttcgctcgcg
     1741 agcggcgcgc tgccccggtg ggccgccggc gccacgtcgg ttgccgccaa cgtgtttgca
     1801 gtgctgctcg tggcgctgct gcttacggtc gcctggaatc ccctgggccc cgagcgcggt
     1861 ttcgtgcgaa acgccgcgtt tgttgtggct atcattggcg ggctgctggc gctgttcttt
     1921 gtgttccagc ggtcttatgc ccggctcctt cggtggtttc tcgaccacaa agcggtatat
     1981 ctggtcgcgg cgctggccgt agtgctggca ggcgctacgg cctggctcgg atttgaccgc
     2041 gtattcgggt ttgtcccggc cgtgctgggg cgtacaggcg ttccgcagga acgcatccgc
     2101 accaaccgcc tctggagcac cgcaatccat gcgttccccg gcttcggcaa ggagttcatg
     2161 cctcccctgg acgaaggttc gtacctgtac atgcccacca cgatgccgca cgcatcgctc
     2221 ggtgaggcgc tggacgtcct tcaataccag gatcaggcca tctccgcaat cccggaaatt
     2281 gatatggtcg tcggcaagat cgggcgcgtc gagagcccgc ttgatccggc gcctgtttcc
     2341 atgatcgaga ccgtcatcca atacaaaccc gaatacatca gcgacgcatc aggacgccgc
     2401 atgcgtttcc ggtatgaccg caagcgcggc gcatttgacc gggatggcaa cggccagttg
     2461 attcccgatc cgggcggacg gcccttccgt cagtggcgcg atgaaatccg ctcgcccgac
     2521 gacatctggg acgagatcgt gcgcgcggcc gagatgcccg gcaccaccac ggcgcccaag
     2581 ctccagccca ttgccgcgcg ccaggtgatg ttgcagagcg gcatgcgcgc gcccatgggt
     2641 gtgaaaatca aggggcccga cctcgagacc atcgagaccg ccggcctggc cctggaacgc
     2701 ctgctccgtg aagtccccag cgtggagccg gacgcggtca tcgccgatcg catcgtcggg
     2761 aaaccctatc tcgagattga catcaaccgc gaagccatcg cgcggtacgg catcagcatc
     2821 cgccaggtac aggacgtcat cgaggtcgcc attggcggcc agcgcatcac cactaccgtc
     2881 gaagggcgcg aacgctaccc cgtccgtgtg cggtatctgc gcgaactacg cgaccagatc
     2941 gaaacactgg aacgaatcct tgtgccttcg ccctccggcg cccagatccc cctggttcag
     3001 ctggccaaaa tccgttacgt gcgcgggccc gacgccatca aaagcgaaga cacctttctc
     3061 gtcggctatg tgctcttcga tcgaaaaccg gaatacgccg aggtcgacgt ggtcgaacag
     3121 tgccggagct tcctcgaaga gaagctcgcc tccggtgagc ttcacctgcc cccgggcgtc
     3181 agttaccagt ttgcgggaac ctatgaaaac caggttcgct cgcagaaaac tctggcgctg
     3241 gtgctccccc tggcgctgtt tatcattttc atgatcctct atttccagtt ccgatccgtg
     3301 ccgaccgcgc tgctggtgtt ttcgggcatt gcgctcgcgt ggtcgggcgg atttctactg
     3361 atgtggctgt acggccagcc gtggtttctc gatttcagcg tgtttggcgc gaacctccgg
     3421 gagctgtttc aggtacgcga gatcaacctg agcgtggcga tctgggtcgg attcctcgcc
     3481 ttgttcggca tcgcatcaga cgacggcgtg gtcatcgcca cgtttctgga tcagagcttc
     3541 gcaaaacacc gccccgaatc catccgcgag atccgtgacg cgaccgtggc cggcgcccag
     3601 cgccgcatcc ggccctgcct gatgaccacg gccaccacga ttctcgccct catccccgtg
     3661 ctcacctcga cgggacgcgg cgcggacatc atggtcccca tggccattcc gtcgttcggg
     3721 ggcatggtgg tggagctgat gacgatcttt ctcgtgccca tcctctattg cggggtacag
     3781 gagtttcgcc tccgcagggg ccgtcccgtc cttcccgccg ccctcgaatc tgaacgtgcg
     3841 gccgtagcgc acgcaacgcc cgcggataag gagtacccat catgatgaat ctgctgtttg
     3901 tggcagcgct cgccgctgct ggcgtcgagg cgcccgcctt ctacgctggt aatgacgaat
     3961 tgcgcggact tctgctcgaa gccgccgaaa acaatcccac ccttctggcg cggtacgaga
     4021 cctggcgtgc ggccctggaa cggattcccc aggtcacggc gctcgacgac cccagcctgt
     4081 cctatggaca attcatccag tcggaatcca gccgggcacg gtttgccgtg agccagagct
     4141 tcccctggtt cggcacgctg cgcgcgcgcg gcgacaaagc cgccgcggaa gccgacgccg
     4201 ccctccaggt ctttttcgca gagcgaaacc gcgtgtttgc cgaggtcaaa aaggcctatt
     4261 tcgagtacgc cttcctggcc gacgcggtcc tggttacgga agcgcagtcc gaagtccttg
     4321 aatatatgga ggaggtggtg gaatcgaagc tcgccctcgg cctggccaat gaggacgagc
     4381 tgctccgcgt gtccatggag aaggcccagg tgcgcgaccg tcgcgacagc tacctccaga
     4441 acaaacccgc gcgcatggcc cgtctgaacg aggtcctggg ccgtgatgtg ctcgaggaaa
     4501 tcccgtggcc ccaatctaca ccgctgccgc ccgaactgcc cccggccgaa accgtcgtcg
     4561 cgcgcatcac agaggccaac ccggacctca aggccctcga ccgcctgctt gaaagccggg
     4621 agcacgacat cgaactcgcg cgcaaggaag gttatccgga attcactgcg ggcctcgaat
     4681 acctggcgat cagcaagccc cgtcaggttg ggcctgatcg cataactgcc gataaccttt
     4741 ccaccggata ccgcctcctg aacaccttca cgggaaggat gccgttcgag cccgtcaata
     4801 ccgccatcga cgcctatggc ctggccacca gccgggaacc tatgaactat tcggacggcg
     4861 gagacgacga aatcatgctc tcgttcacgc tcagcgtgcc ggtctggcgg aaacgggtca
     4921 aggcgggtat tgaggaggcg cgcctgctgc gccgcgctgc tgaacacgac aaacgcggcc
     4981 gcgagcttgc gttgcaggcc tcggcgcaaa tggccgtgtt tgaagccgag gacgcgctgc
     5041 gccgttaccg gttgtaccgc gacacgctgg tgccccaggc cgagcagaca tacgaaagcc
     5101 tgcaggacca gtactcggct gccaccggcg gcgccgactt tctcgacctc cttgaaagcg
     5161 tgcaaaccct gctcgctttc gaactggaac aggttcgggc gtcgcgcgac gcccagcttg
     5221 ccgcgtctga cctggaattc ctgatgggcg gtccgtggtt gctaaacaac ggcggcagcg
     5281 agcccgcgag tgagggcgtt tcagggcagc aggcgccgta ccgaatcacc gtcagaaaaa
     5341 gtgaacataa cgaatcggag gaagacccat gacacgcatg attatgatta tgtcggcggc
     5401 cgcgatggca ctgaccggtg cggccgcaca cgcgggggaa gccagcgcgg acgccccggg
     5461 cagcatctac acgctcgcga cctgtccggt tacgggcgca gccctgggct ccatgggcgc
     5521 gccaattgtg aaaaagtacg atgggcggga ggttcgcttc tgttgcgccg ggtgcgtctc
     5581 atcttttgag gcggataagg aagcctattt caaaaaaatc gacgcagcag tgatcaatga
     5641 gcagaagaaa agctatccgc tcgacacctg tctcgtgagc ggccaaaagc tggggtccat
     5701 gggcgaatcc gtcgacctgg tgtatcgaaa ccggctggta cggctgtgct gcgaagggtg
     5761 ccgcgcggcg ttccagaaag agcccgcgaa atacctcaaa aaactcgacg ccgccattgt
     5821 cgaaaaacag aaaggcgcct acccggtcga cacctgcgtg gtatccggag agaagctcgg
     5881 tgcgaaaggc gaccccgtcg actacatcgc gggcggccgg ctggtgcgat tctgctgcag
     5941 cggctgcgtc aaggagttcg aaaaggaccc ccggacatat ctcgacaaac tcgacgccgc
     6001 agccaagcca tcgaacggat gacctgaaca acaggctggc tcctgcacag cggaaaagtg
     6061 ctgcgccgga gccggcccgt ctcgaaagat gcacttgtcc aacacggcgt cttttcccgc
     6121 atgaatttcg cgcgggcgcc gtcctgtgcc gtggccggcc gccgtgacga caggcccccg
     6181 gtatgtctgc tatgctgtcg ccagacatgc gtatccgtat cggtgttctg gctgcggcct
     6241 tcctcaagga ggattccaag gtgagacctg tatcacggct cctggcggcg ttgctggtgt
     6301 gcgcggggtg cgcccacgtg ccggtcaacg aaaacctcac gctgtttcag ggcgacccca
     6361 tcgaaaatgt gcagggagtg agcttcgacg gcgaggtatt tgaggtgcct ggcgcgggtg
     6421 cgatttcccg cgacgaggtc accgagctcc tgttccagcc tgcggaagcg gaaggcggga
     6481 aggcagccgc gccggccggg gtggccggcc tcaccggtct ggcgcaggag atgctcccga
     6541 aaggccgggc gctggccgag cagttcccgg gcacgggcgg ggtgattctg atcgatgacg
     6601 gcctcttcgt gtacaacgac gacggcacca accggtaccg ctaccatttc gcggggctgg
     6661 tgctcaagga agagatgaaa tcatgggcgc agttcgcggc ggggttcact gagggccgga
     6721 gccgggtccg ggtgctggaa gcgaagtccg tcgcacccga tggcacgatt tatactcttc
     6781 cggcggaggc actgcgcgta ggaagcccct ccgaagagat gcagttcttc aaccccagcc
     6841 gcaaggtcgt gggcggggtc attccgggcg tcgagatcgg ctcgatcgtc gaatacatct
     6901 acgaatacga gaattataac cccgaagact cgcgcctgtt tctgccgggg tactattttc
     6961 aatccaccga gcccgtggcc ctgtcgcggg tgaccatcga ggtcccggat cggctgccgt
     7021 tccatcatta cacgcggcat tttcccgacc cggcgctggc cgagccgcag gtggagcgcc
     7081 acggcaggcg ggtgcactac gtctgggccc tgcacgacat gccgccgctg gtgccggaac
     7141 cgatgatgcc gccgcagacc gatctcacgc cgcgcatgga ggcctcgatc tttgcctcgt
     7201 acgaggaggt gtttgcgctg cagcgcgacc tccagcgctc ccgcatgaaa ctgacgccgc
     7261 aaatcgaggc ggaagtggcc cggatcaccg aaggggccgc atccatcgac gaaaaactcg
     7321 cccgcattta ccactgggtt cagaccaata cccggtacat ctcgatcaag ggaagcctcg
     7381 gctccggact ctccggccat acggccatgg agacttacga gaaccggtac ggcgactgca
     7441 ccgacaaggc catcctgttc gccaccatgt gcgaggccat cggcgtcacc agctatccca
     7501 tcatcctctc gaccaacgac aacggcgtcg gggtgacgga aatccccacc attgacggca
     7561 atcacgccat atccgaggtc gatcttcccg atggccgccg tttctatctc gattcgacgg
     7621 cgcagaattt ccggtatcct tatttccgcg ccgatgacca cggttgcgtc gcggtcaacg
     7681 caatccgggg cgatttcaac atcattcccg tacccccgcc cgaagacaac gcccgccatt
     7741 cggagctcga ggccgccctg gcggccaacg gcgatatgac cgtgcgcgca cggaatgagt
     7801 ataccggcac catcgaagcg ggtatccgcg ccttttggaa gactgtgcgc gacgacgagc
     7861 gcgccgaccg catgaccgaa tatgtgaact ccttgagtcc aggcgccatc ctcgacgact
     7921 ttagcctgtc ggaattgacg gaactcagcg aaccggtgcg catgggcatc gactaccgtc
     7981 tgccgggcca tgcgatccgc gcgaaaaagc tgatgtatct gcagatgccg tcgctggagc
     8041 ggacttttcc tgaggtggcc ctggaatccc gccgcttccc catccaatac atgacgaccg
     8101 aagagcggac cctcaccatc cacctcgcat tgccggccgg gttccggctg aagtgggcgc
     8161 cgccgcccct gcgcatccgc acgccgtacc tcgaatacga cgccgcctat gaggagaccg
     8221 gcgacggcgt cacgttgaag gaggtgttcc ggcggctgga caggatcatc ccggtcgagg
     8281 cctaccccga ataccgcgat ggactgcggg ccattg
//
LOCUS       DAOPBQ010000087         8309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7962_length_8309_cov_4.494912, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000087 DAOPBQ010000000
VERSION     DAOPBQ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7962_length_8309_cov_4.494912"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKW60_04280"
     CDS             <1..313
                     /locus_tag="PKW60_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HPJ98678.1"
                     /translation="LRATFPTGTVSGAPKIRAMEIIDELEPVRRGPYAGGCGYISFSG
                     DMDTCIIIRTMIVKDGTAYVQAGAGIVSDSVPEREYEETVNKAKALFRAVEFAERGLE
                     S"
     gene            639..1232
                     /locus_tag="PKW60_04285"
     CDS             639..1232
                     /locus_tag="PKW60_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98679.1"
                     /translation="MTQPPKNLGKKQQRALAHLDRNLQLSREFLNDWLLFNQILSAFP
                     AENSNRPELENQFLKIKSKLAREHQILRESLGQDYRIDGNTMNIVAGATSLENIFSQS
                     EIAVKKLQGEWHRAFISINETLGGLEDKRSRVLGGEKVFVGGLDSPTTGGGGISDKTK
                     KTVIIIVVLLIIVGIMVGVPQIRDMYMDAFKDLFGGK"
     gene            1340..1903
                     /locus_tag="PKW60_04290"
     CDS             1340..1903
                     /locus_tag="PKW60_04290"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPJ98680.1"
                     /translation="MGRKAAGPGKRRGVRTARGGLNRADPAAGHAVHTQPADRGGGAR
                     TAVAVRQGKENNVTKRELVIEIAEQLGYTQNEVADVVQMTLDTITECLAEGHRLEIRN
                     FGVFEVKQRDARMGRNPRTGQEVPITHKRVASFKPGKALKERVQATVGKAEPAPETTP
                     AWPPAEEAPEDNSNVAFPGSGEQGRLF"
     gene            2077..2970
                     /locus_tag="PKW60_04295"
     CDS             2077..2970
                     /locus_tag="PKW60_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPJ98681.1"
                     /translation="MSEQTNNSDYEARMTFTEHLAELRIRIIRAGIAAILGMFVAYAV
                     GAYLEDALMWPIIGFMAEKEHPAGQGEALAPSTPADGESATPPADGAATRGSQPATPR
                     VRQQWTNFLAPVLLRLKIAAYAGVLLVSPYIIYQICAFIFPGLKPNEKKAVRILLTGS
                     GVLAIVGVCVAYFGILPVVIPYLLNTFISPEIELNLRRDETIPIIVKLLTGFAIAFQF
                     PMVVLVLVYLDLLSPQALKKYRKIAVVILAVASAVLTPPDVFSMIVMLLPLLILYETS
                     IVLSYLVVRSKKKKKAARASA"
     gene            3002..3466
                     /locus_tag="PKW60_04300"
     CDS             3002..3466
                     /locus_tag="PKW60_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPJ98682.1"
                     /translation="MLGMGMGEMILVAGIALIIIGPERFPEFAKICMRTIRDLRSYVE
                     EAKEDLRREIAPLKKEIDQIKSYDPESYIDALTDSRDDSGHYRASSTQKTGESAGTNA
                     STTTSTKPHADEKKPETGSAVAKADIAAAAPASPETTSTEEENAHIPEPMDG"
     gene            3578..4630
                     /locus_tag="PKW60_04305"
     CDS             3578..4630
                     /locus_tag="PKW60_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98683.1"
                     /translation="MTSPIGIGVLSFAHGHVSLYADVMAGFPEVRLLAAYDDNAERAR
                     PVCERTGMRYENRPEGVLSDPAVQAVMIGSETSRHAALCIAAAEAGKHILLQKPMAMT
                     LEECDRMNAAAAAHGVTLAMAWQMRHDPANIKMRQLVQSGALGKIGVVRRRHCIPVLF
                     QPEFINGPTNWHIRAETNIGMFADDAAHPIDWLCWTFGKPVSVMAEIDNVLTDVAPDD
                     NGIALFRLANGAMATIFNSSTVDAGECTTEIYGDQGVLIQNYGDGPSCSIPGCPEGHA
                     LKLYRYDTHSWTPYDIPKPASQGERIAAVPRPWVDSLVSGAAPAATGIDGRLALEVVL
                     AAYQSAREGRRIVLGP"
     gene            complement(4627..5832)
                     /gene="coaBC"
                     /locus_tag="PKW60_04310"
     CDS             complement(4627..5832)
                     /gene="coaBC"
                     /locus_tag="PKW60_04310"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPJ98684.1"
                     /translation="MKRITSNKEVVLGVTGSIAAYKACELASRLIEAGYTVTPVLTAS
                     AQKLVGPATFEALTGRRAITGMFEPFASPEIEHIAVAQRAALFLIAPATANIIAKAAH
                     GIADDWLSTTLLATRAPILIAPAMNTHMYNHPATQENIAILKARGCAFAGPGSGRLAC
                     GTVGPGRLIHIPRILEAVEVLLHQTKDLAGLSVVLTAGGTREPIDPVRYIGNQSSGRM
                     GRALAMEALKRGARVTVIAGHMDVAPPEGAEVIVAETAKDMAEVALSLAQDADIFIGA
                     AAVGDFRVENPSGDKQKRSGKGLQINLVENPDVISQVGALRRDGQLVVGFAAETTDLI
                     KNAKAKIKQKQLDLIVANQVGVEDSGFGTETLRASFIDSKGSVDELELLTKEEVATLL
                     FDRIASLLG"
     gene            complement(5810..6034)
                     /gene="rpoZ"
                     /locus_tag="PKW60_04315"
     CDS             complement(5810..6034)
                     /gene="rpoZ"
                     /locus_tag="PKW60_04315"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPJ98685.1"
                     /translation="MGKMYSPEDFDDKADSVYRLVLAAARRASQLAKPESRPLVPVRS
                     KKPTMVALEEILEGKVAVRETKTDEEDYFE"
     gene            complement(6125..6721)
                     /gene="gmk"
                     /locus_tag="PKW60_04320"
     CDS             complement(6125..6721)
                     /gene="gmk"
                     /locus_tag="PKW60_04320"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018001097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPJ98686.1"
                     /translation="MCAVKTGEVFVVSSPSGAGKSAILHRVREADPTLLFSVSATTRL
                     PRAGECDGRDYYFVPQEEFRRRVDAGEFLEWAEVHGNLYGTLRAEMERLAASGQDAVV
                     EIDVQGAASLRALGFRMVSVFIIPPSLAELERRLRARGTEAEDVLALRVKNARGEIDQ
                     YRQYDFVIVNDDLDTAVADFQAIVRAQRCRAERFEDRP"
     gene            complement(6725..7591)
                     /locus_tag="PKW60_04325"
     CDS             complement(6725..7591)
                     /locus_tag="PKW60_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPJ98687.1"
                     /translation="MTGFGKAACTCQEQEISLELSAVNHRYLDCSFRLGNSWASLEPA
                     LKGVVRNRISRGKVQVTVNRRRAKTCTQTVHFDAGLAQQYLGAFHELAAMIGSDGPVG
                     LDTLARMDGVFYQEEPDEDLAEVERALTGAIEEALQHLNAMRAQEGAALAEDIKARIQ
                     LMRDSLAEIEARLPELNAKHEERLRARIQELAADIALTEERVAIEVALLADKGDVTEE
                     AVRLKTHLAHMEELLDSDAAVGRRLDFLSQEIQREVNTLGVKTRDCDVAKYVLDMKSE
                     LEKIREQVQNVE"
     gene            complement(7794..>8309)
                     /locus_tag="PKW60_04330"
     CDS             complement(7794..>8309)
                     /locus_tag="PKW60_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98688.1"
                     /translation="ATPQQVRAARERLRTHTLDPRDIETIYAHCLLRVENMRKARARE
                     RLEIMGVRIADACFWGVPGEYFQEFAMNVRKTSEFPHTCCVELANGYFGYICTEEAFA
                     GGGYEIRTAPSSYLVEDTGRRIQKTGERVVSELCRAAQTELARLPQMRFWDSHDPDDA
                     LGAIHKIKEKR"
BASE COUNT         1673 a         2576 c         2412 g         1648 t
ORIGIN      
        1 gctgcgagcc actttcccga cgggaacggt aagcggcgca ccgaagatcc gcgccatgga
       61 aatcatcgat gaacttgagc ctgtgcggcg ggggccgtac gcgggcgggt gcggctatat
      121 cagtttctcc ggcgatatgg acacctgcat catcatccgc acgatgattg tcaaagatgg
      181 aacagcctac gtgcaggcag gcgcgggaat tgtcagcgac agtgtccccg aacgcgagta
      241 cgaggaaacc gtaaataagg cgaaggccct cttcagagcg gtagaattcg cggagagggg
      301 gcttgaatct taatcgccgt gcaccggtca ttttacctgc tcccgccgca gcctcgtccg
      361 gcatctgggc ggggcgggaa accgcgcccg ggcgttccag aacaatgcca ctgaggtggc
      421 cgggattcgc tccacaagag aagaccagcc gtcttgcggc acggccgggc ccgcaggtgt
      481 caaaccattt tctttgccgc agaggagaaa aaacagaggg gcaaatctgc gctaagccat
      541 gaaaattgca tgacttatac ctttgacatt gggcagatcg tgtgctaaag taccgttgaa
      601 atgggcagtt tcacttccgg atagaaaggc gactcgaaat gacgcagcct ccgaagaatc
      661 tggggaaaaa gcagcaacgg gcactggcgc accttgaccg caatctgcag ctgagccggg
      721 aattcctgaa cgactggttg ctcttcaacc aaatcctctc cgcgtttcca gcagagaatt
      781 ccaaccgacc ggaattggag aatcaattcc tcaagatcaa gagcaagctc gcccgggaac
      841 atcagattct tcgcgaaagc cttggtcagg attaccgtat tgacggtaac accatgaata
      901 tcgtcgcggg agcgaccagc ctcgagaaca tcttcagtca atcggagatc gccgtcaaaa
      961 agctgcaagg cgaatggcac cgcgccttta tctccattaa cgagacgctg ggcgggcttg
     1021 aagataagcg cagccgtgtg ctcggtggtg aaaaggtctt tgtgggcggc ttggattcgc
     1081 cgactacggg cggcggcggc atatcggaca agaccaagaa gaccgtgatc atcatcgtgg
     1141 tgctcctgat tattgtcggg atcatggtcg gtgtgccgca gatccgggac atgtacatgg
     1201 atgcgttcaa ggatcttttc ggcggcaagt agcgcactgt tgcggatgac cgcgtcccca
     1261 ccgaatgaac atcagaccag gcgccctgga cgcagaggat aggtgacgct ggcgttggcg
     1321 ccccggcggc tgggttgcga tgggccggaa agccgctggc cccgggaaaa gaagaggcgt
     1381 acggacggcg cggggcggcc tgaaccgggc ggatcctgcg gctggccatg cagtgcacac
     1441 gcagcccgcc gaccggggag gcggcgcccg tacagcggtt gccgtgcggc aaggaaagga
     1501 gaacaacgtg accaaacgcg agttggtgat cgagattgcg gaacagctcg gctacaccca
     1561 gaacgaagtc gcggatgtcg tccagatgac cctggatacc attacggaat gcctggccga
     1621 aggccaccgc ctcgaaattc gaaattttgg cgtttttgaa gtgaaacagc gcgatgcacg
     1681 catgggccgg aatccccgga cgggccagga agtgcccatc acccataagc gggtggcgtc
     1741 gttcaagccg ggtaaagccc tgaaagagcg cgtgcaggca acggttggca aggccgagcc
     1801 tgccccggag accacacccg cctggccccc tgccgaagaa gcccccgaag acaacagcaa
     1861 cgttgccttc ccgggcagcg gcgagcaggg ccgcctgttt tagctgccgt cctgcggcgg
     1921 gatccttccc tcccgggctg atttggacgc aggggctcgc ggagtatata ctaccgattc
     1981 ctgggttgcg tcctttgcac gattgcttcc cgcctgcaga tcttctgagg ccggtgacgg
     2041 ccacgtaaac cgcggccaca ttgggtttcc attcagatgt cagaacagac caataacagc
     2101 gactacgaag cgcgcatgac cttcacggag catctggcag agcttcgcat acgcatcatc
     2161 agagcgggaa tcgcggctat ccttgggatg tttgttgcgt acgcggtggg cgcctatctg
     2221 gaagatgccc tgatgtggcc gatcatcggc ttcatggccg agaaagaaca cccggcgggt
     2281 cagggagagg cattggcacc gtccacgccc gcggatggtg aaagcgcgac gccgcccgca
     2341 gacggcgccg ccacacgagg cagccagccg gcgacccctc gagtccggca gcaatggaca
     2401 aatttcctcg ctccggtgct tctccggctg aagatcgcgg cctatgccgg ggtcttgctg
     2461 gtttcaccct atatcatcta tcagatttgt gcgttcattt ttccgggact caaaccaaac
     2521 gaaaagaaag ccgtacgcat tctgcttacg ggcagcggcg ttctggcgat tgtgggtgta
     2581 tgtgtggcct atttcggcat actgccggtg gtcatcccct acctcctcaa caccttcatc
     2641 tcgccggaaa ttgagctgaa tttgcgccgc gatgagacca tccccatcat cgtgaagctg
     2701 ttgacgggct tcgccatcgc gttccagttt cccatggtgg ttctggttct ggtctatctg
     2761 gatctgcttt ctccccaggc gttgaagaag tatcgcaaga ttgccgtggt gattctggcg
     2821 gtggcgtctg ccgtgttgac tccgcccgat gtgttttcga tgatagtcat gctgctgcct
     2881 ctgctgattc tgtatgagac aagcattgtg ttatcgtatc tggtggttcg cagcaagaag
     2941 aagaaaaaag ccgcgcgcgc cagcgcatag acccgcgcgc ataacttgag aagccgacag
     3001 catgcttgga atgggtatgg gagagatgat cctcgtggcc gggatcgcgt tgatcatcat
     3061 tggcccggaa cgatttcccg agttcgcgaa aatctgtatg cgaacgatcc gtgacctgcg
     3121 ctcgtacgtc gaagaagcca aagaagacct ccggagggag atcgcacccc tcaaaaagga
     3181 aatcgaccag atcaagagct acgaccctga aagctacatc gatgccctga cggattcccg
     3241 cgacgattcg ggccattacc gtgcctcatc cacacagaaa accggcgaat ccgccgggac
     3301 gaacgccagt accaccacca gcacgaagcc gcacgcggat gaaaagaagc ccgagacagg
     3361 ctccgctgtg gcaaaagcag atatcgccgc tgccgctccg gcttcccccg agacaacatc
     3421 cacggaggaa gagaacgctc acatcccgga accgatggac ggctgacctt ccttccctcg
     3481 cgaaattgcc cgactgcgct ctgagcgagt gtccctttac ttggaagctc cgcgtgtttt
     3541 acattacgct catccaccac agatggaagg aaaagccatg acctctccca ttggaatcgg
     3601 cgtgctgagc tttgcgcatg gccatgtcag cctgtacgcg gacgtgatgg cgggatttcc
     3661 ggaggttcgc ctgctcgccg cctatgacga caacgcggaa cgggcccggc cggtctgcga
     3721 gcgcacggga atgcgttacg agaaccgccc cgagggggtg ctgtctgacc ccgcggtcca
     3781 ggctgtcatg atcgggagcg agaccagccg ccacgccgcg ttgtgcatcg cagccgccga
     3841 agcgggaaag catattctgc tccagaaacc aatggcgatg acgctggagg aatgcgaccg
     3901 gatgaatgcc gcggccgcag ctcatggcgt cacactggca atggcctggc agatgcgcca
     3961 tgatcccgcg aacatcaaga tgcggcaact ggtccagtca ggcgcgctgg gaaaaatcgg
     4021 cgtggtgcgg cgccggcact gcattcccgt cttgttccag cccgagttca tcaacggccc
     4081 caccaactgg catattcgcg cggaaaccaa tatcggcatg ttcgccgatg acgccgccca
     4141 ccccatcgac tggctgtgct ggaccttcgg caaacccgtg agcgtcatgg ccgagatcga
     4201 caatgtactg actgacgtgg ccccggatga caacggaatc gcgcttttcc gtctcgccaa
     4261 cggcgctatg gcaacaatct tcaacagctc aactgtcgac gcgggcgaat gcaccacgga
     4321 aatttacggc gatcaaggcg tgcttattca gaactacggt gacggcccgt cgtgcagcat
     4381 acccggctgc ccggaagggc acgcgttgaa actgtaccgg tacgatacgc actcctggac
     4441 cccatacgac atcccgaaac cggcgagtca gggcgagcgc attgctgccg tgccgcggcc
     4501 ctgggtggac agtctggtga gcggcgcggc gcctgccgcg accgggatcg atgggcgcct
     4561 cgcgctggaa gtcgtgctgg cggcctatca atccgccaga gaaggccggc gaatcgtatt
     4621 ggggccctaa cccagcaggc tggcgatacg atcaaacagg agcgtggcca cctcttcctt
     4681 cgtcaggagt tccagctcgt ccacgctgcc tttggaatca atgaaactcg cgcgcagcgt
     4741 ctcggtacca aatcctgaat cctcgacgcc gacctggttg gccacgatca gatccagctg
     4801 tttttgtttg attttggcct tcgcgttctt gatgagatcc gtcgtttcgg ccgcaaatcc
     4861 cacgaccagc tgcccatcgc gccgcaacgc gcccacctgg ctgataacgt ccggattttc
     4921 gacgagattg atctgcaggc cttttccgct gcgtttctgc ttgtcgcccg atggattttc
     4981 aacccggaaa tcgccgactg ccgccgcacc gatgaagata tcggcgtcct gggccaggct
     5041 caaggccact tcggccatgt ccttcgcggt ttccgccaca atcacctccg cgccttccgg
     5101 cggcgctacg tccatatggc ccgcaataac cgtcacgcgc gcgcctcgtt tcaaggcttc
     5161 catggccagg gcccggccca ttctgccgga cgactggttc ccgatatacc gtacggggtc
     5221 aatgggttcg cgggtaccgc ctgcagtcag cacgacagac aggcctgcca gatccttggt
     5281 ttggtgcaac aggacctcga ccgcttccag tatccgcggg atatggatca ggcgcccggg
     5341 cccgactgtg ccgcaggcca gcctgcccga acccggcccg gcgaacgcgc acccccgcgc
     5401 tttcagaatc gcgatattct cttgcgtcgc cggatggttg tacatgtggg tattcatggc
     5461 cggcgcaatg agaatcggcg cccgggtggc caggagggtc gtactcagcc agtcatccgc
     5521 gatgccgtgc gctgctttgg cgatgatgtt ggcggtagcg ggggcgatca aaaacagggc
     5581 cgcacgctgc gccacggcga tatgttcgat ctccgggctt gcgaaaggct caaacatgcc
     5641 ggtaatcgcc cggcggccgg tcagggcctc gaatgtggcc ggtccgacca gcttctgcgc
     5701 cgacgccgtc agcaccggcg tgaccgtgta accggcctcg atcaggcggg atgcgagctc
     5761 gcacgccttg taggcggcga tcgagcccgt cacgcccagg acaacctctt tattcgaagt
     5821 aatcctcttc atccgttttg gtctctctca ccgcgacctt gccttccagg atttcctcca
     5881 gggcgaccat ggtcggcttt ttcgagcgca ccggaaccag cgggcgcgac tcaggttttg
     5941 cgagttggga ggcacggcgc gctgccgcga gtaccagccg gtacacgctg tcggccttgt
     6001 cgtcaaaatc ttcgggggag tacatttttc ccatgctgat ccttccttct tacgtccagc
     6061 ctcgagcttt ttatcgccgt gggcgttcag tccaagccgg ccgatcgcgg ccgcgcccgg
     6121 ctcctcaagg tctgtcttca aaacgttcgg cgcggcatcg ctgcgcacgc acaattgcct
     6181 gaaaatcggc cacggccgta tccaaatcgt cattcacaat gacaaaatcg tattggcggt
     6241 actggtctat ctcgccgcgg gcgttcttca cccgcagcgc gagcacgtcc tctgcctcgg
     6301 tgccgcgggc ccggagccgg cgctccaatt ccgccagtga cggcggtata atgaacaccg
     6361 acaccatcct gaaccccagt gcgcgcaggc tggctgcgcc ctgcacgtca atctccacta
     6421 cggcatcctg cccggaggcc gccaggcgct ccatttccgc gcgcaaggtg ccgtacagat
     6481 taccgtgcac ctcagcccat tcaagaaatt ccccggcatc cacgcgccgc cggaactctt
     6541 cctgcggcac aaaataatag tcacggccgt cgcattcgcc tgcacggggc aagcgcgtgg
     6601 tcgccgatac cgaaaacaag agagtcggat cggcctcccg gacacggtga agaatggcgc
     6661 tcttgcccgc gcccgaaggc gaagacacca caaacacctc gcctgttttc actgcgcaca
     6721 tcatctattc gacgttctga acctgttccc gaatcttttc cagttcactt ttcatatcca
     6781 gaacgtattt cgccacgtcg cagtcacggg ttttaacacc aagcgtgttg acctcgcgct
     6841 ggatctcctg cgacagaaaa tccagccgcc ggcctaccgc ggcatccgaa tccagcagct
     6901 cttccatgtg cgccagatgg gtcttcagcc gtacggcctc ctcggtcaca tcgcccttat
     6961 cggccaggag cgccacctca atcgcgaccc gttcctccgt caacgcaata tccgccgcga
     7021 gttcctggat tcgcgctcga agccgttcct catgcttcgc gttcagctcg ggcagccgcg
     7081 cctcaatttc agccaggctg tcgcgcatca gctgaattcg ggctttgata tcttccgcca
     7141 aggctgcgcc ttcctgggcg cgcatggcgt tcagatgctg caacgcttcc tcaatcgccc
     7201 cagtcaaagc ccgctcaact tccgcaagat cttcgtctgg ctcttcctga taaaacacgc
     7261 catccatccg cgccagagta tccagcccga caggcccatc cgacccgatc atcgcagcca
     7321 gctcgtgaaa tgcgcccagg tactgctgtg ccagcccggc atcaaaatgg actgtctgtg
     7381 tgcaggtctt cgcccggcgg cggttcacgg tcacctgaac tttgccgcgc gatatacggt
     7441 tgcgcactac gcccttcaac gcgggttcaa gggatgccca tgaattgccc aatcggaatg
     7501 aacaatccag ataccggtga ttgacagcgc tcagctccag cgaaatttcc tgttcctggc
     7561 aagtgcatgc ggccttgcca aaaccagtca tacttcgtat catctgcgct cccttatgaa
     7621 atgacggtgt ttgcacctgc cggacaaagt cacctaagta taccgatttc aagcacgtat
     7681 ggcaagtttt gcgcctgctg tttcccggcg ggagctcgca cgtgccggca ggcactcgct
     7741 ccgtagagac gtgtttttct ggatttttac cgtacgtggc ctgctgccgc cgctcaacgt
     7801 ttttctttga ttttatggat ggctccgagc gcgtcgtcag gatcgtggct gtcccaaaag
     7861 cgcatctgcg gcaagcgggc cagttcggtc tgcgctgccc ggcacaactc cgaaacgacg
     7921 cgttcgcccg ttttttggat gcggcggccg gtatcttcga ccagatagct gctcggggcg
     7981 gtgcggatct cgtacccgcc gccggcgaac gcctcttccg tgcagatgta gccgaaatag
     8041 ccgttggcca gctcgacaca gcacgtatgg ggaaattccg atgttttgcg gacgttcatt
     8101 gcgaattcct gaaaatattc gcccggcacg ccccagaaac aggcatcggc gatgcgcacc
     8161 cccatgatct ccagccgttc tctggcgcgc gcctttcgca tgttttcgac gcgcagcagg
     8221 cagtgtgcat agatggtttc gatgtcccgt gggtcaaggg tgtgcgtgcg aagccgttca
     8281 cgggcggcgc ggacctgttg cggcgtggc
//
LOCUS       DAOPBQ010000088         8292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_7979_length_8292_cov_3.797985, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000088 DAOPBQ010000000
VERSION     DAOPBQ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7979_length_8292_cov_3.797985"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKW60_04335"
     CDS             <1..437
                     /locus_tag="PKW60_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HPJ98689.1"
                     /translation="AWLINFFARLHDGEAAYENLRLLLSKSTLNNLFDNHPPFQIDGN
                     FGGTAGIAEMLIQSHTDEIVLLPALPTAWKDGAVSGLVARGGFEIGMKWRDGRLESAA
                     VHSRLGNPGTLRYGPYTAALAGEKGERALFDGTLQPAAVRAR"
     gene            520..1539
                     /locus_tag="PKW60_04340"
     CDS             520..1539
                     /locus_tag="PKW60_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPJ98690.1"
                     /translation="MAATSRERVYQALQFEDPDRAPRHLWTLPWAELHFPGELSQLRE
                     DYPDDLVSAPGFCQETARTDGDEYAVGRYTDAWGCVFDNLQPGVIGEVKQPLIRDWIA
                     DAAKVHIPREWLTIDIDRVNEFCEATQQFVTGGCCPRPFERLQFLRGTASLYTDLLEP
                     PPGMAGFVREMHAFYCQWMEVWAATDVDALNVMDDWGAQRALLIDPVIWRDVFKPLYR
                     DYVHIAHGAGKKVFLHSDGHILAIYPDLVELGFDAVNSQLFCMGVENLAPYAGKTTFW
                     GEIDRQHLLPEATPDEIDRAVSLVHKTLWEKGGCIAQCEFGPGAKPENVRQVFASWDR
                     LTLGG"
     gene            complement(1651..2526)
                     /locus_tag="PKW60_04345"
     CDS             complement(1651..2526)
                     /locus_tag="PKW60_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPJ98691.1"
                     /translation="MTSATLKRTTPMHEFLKALLHPDIPVLRHAVLVGLLSSVAFGIV
                     GSFVVTRRVTFIAAAIAHSVLGGIGAVLYLRAHTGLTWLEPVHGALPAALFSALIIGL
                     VSLRAKEREDTVIGAVWAVGMALGLLLLAKTPGYNKDTMSYLFGDILLITKRDVWMVA
                     GLDILVTAVAVFFYNKLLAVCFDEEFARTRGLAVDYYYLLLLCLIAVTIVLLVYLVGI
                     VLVIALLTLPAAVAGQFSRRMARIMIMAVILCMVFSTAGIALSYPYDLPSGPMIIVTG
                     AAAYLAVLAVKRLRA"
     gene            complement(2486..3283)
                     /locus_tag="PKW60_04350"
     CDS             complement(2486..3283)
                     /locus_tag="PKW60_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98692.1"
                     /translation="MTILTSAEDAASREASPPAVCLHNVSFAYNAQPVLRDVNLDIPS
                     GMFSSVIGPNGGGKSTLLKLILGLLTPDEGQVRVFGQAPATVRGRVGYTPQFAQYDPL
                     FPVSVMDVTLMGLAERHWGGRYTLEEKDAARRVLDEMGIEELWHRPFSDLSGGQRQRV
                     LIARALISSPDLLLLDEPTASVDLAAAKRLMQTLREVAKRRTIVMVSHDPTFIYDLVS
                     RVICVNQRAHIHPTSELTTARLRELYGEDARLIRHDQRDAETDHAHA"
     gene            complement(3280..4221)
                     /locus_tag="PKW60_04355"
     CDS             complement(3280..4221)
                     /locus_tag="PKW60_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPJ98693.1"
                     /translation="MNAKRLMPSGTPLVLFAAVTFVCGCSPAPEPARHGGPGGGPLEV
                     FVSIPPLAALAEAIGGDHVHVTTLLAPGEGPHTFSTTPKQVMALGSASAYFHVGGFPF
                     ELELAKRIEANFPSLVIQDIAEDLARIEDSPQDHEEGAAGHAHADDMDPHVWMSPVIL
                     KAMAATIAETFQRIDPAHADDYARNLTAIEGVLDALDHHLREQLAPFRGRTFYIYHPA
                     LGYFAEAYGLRQKSVEVDGKKPTPRQLQELIEQAQADGVHTIFVQPQFDQRSAKSVAE
                     AIQGTVVPLDPLRADVLDNLRAIGDAFAHALQAERMP"
     gene            complement(4211..4504)
                     /locus_tag="PKW60_04360"
     CDS             complement(4211..4504)
                     /locus_tag="PKW60_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HPJ98694.1"
                     /translation="MSSAKKQEIITFKVDEGLSQAMQGIGNRSEFIRRAILAALENVC
                     PLCLGAGTLSPEQRKHWDHFATTHNVEQCEDCHALRIVCMAGEAPDATGRHER"
     gene            4764..5675
                     /locus_tag="PKW60_04365"
     CDS             4764..5675
                     /locus_tag="PKW60_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPJ98695.1"
                     /translation="MTSFRMISVALLGVALSFSILAAEPEPAVEHVVVIGVDGMTAKS
                     LEVANTPNMHALMALGSYTRAAHSVKPTVSGPNWGSMLMGSKPETHGIVNNSWTLDAD
                     DPFPTVFTALRAADPNAVLVAAYEWDTFGKLFVDEVVTQRIGVDKEAIENDTSENAAS
                     RYIATQAAQCIKEKKPKLTFIHLDMVDHAGHSYVYDSEKYNVAVGEADELVGIVVKGV
                     DDAGIRKNTAIFVVSDHGGIDKGHGGDTPEETIVPWIVAGAGIVKGAAIPDGISVAQT
                     GPTIAALLGAELPARWTMKPVSQVLKK"
     gene            complement(6022..6100)
                     /locus_tag="PKW60_04370"
     tRNA            complement(6022..6100)
                     /locus_tag="PKW60_04370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6062..6064),aa:Pro,seq:tgg)
     gene            6316..6915
                     /locus_tag="PKW60_04375"
     CDS             6316..6915
                     /locus_tag="PKW60_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98696.1"
                     /translation="MRKVLTFIGVVVVVGGVASFNHWYRLSNVINLHTEMAKNVRQSL
                     VDRGMRPISWELLKKTQGEFESIPVYPPELKELDGKQVTVVGYGMPLGGDLPSHDDDH
                     GHAHASMLPRILPLLELFKGHVHTQKYNGVPEVMLTPLPLECYWKRLPPLNQALYVRP
                     ADPIEFADGAVLGFRGTLELREKEGPKFFFVLKNAELLK"
     gene            6992..>8292
                     /locus_tag="PKW60_04380"
     CDS             6992..>8292
                     /locus_tag="PKW60_04380"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPJ98697.1"
                     /translation="MTAKALTDPNQPFEPGTSPLEQSVAALPGIGGKRAELLGNLDIA
                     TIRDLLFYFPRSYEDRRQPRPIAQVNEGDTATIRAVVVRSTTRRLRRKLSATEALLRD
                     DSGEMKAIWFGLGFLSRTLVPGKEGFFTGTAGKWNGLCLRNPDYELLSHDDEDRLHTG
                     RIVPVYRLTEKLSQRMLRRWVRIALDEVRDHVEDRLPPDIAGRWPHTDVRRAIEAVHF
                     PATMEEARKARERFAYEELLGIQLGILQGRSRRAHEEVGYRHVTSGPRLNALRRSLPF
                     PLTQGQERAVEDILADMASARPMVRLLQGDVGCGKTVVALHAIAAAVDGGFQAALMAP
                     TEILAEQHALSLRESLEPLGMTVDVLTGSTGNARAVRQLTTGGNVDVLVGTQALIQEK
                     TGFRNLGLVIIDEQHRFGVVQRDALVQKGVRPDILHMTATP"
BASE COUNT         1644 a         2529 c         2564 g         1555 t
ORIGIN      
        1 gggcgtggct catcaatttc tttgcgcggc ttcacgacgg cgaggcggct tacgaaaatc
       61 tgcgcttgct tctgagcaaa tcgacgctga ataacctgtt cgacaaccac ccgccgttcc
      121 agatcgacgg caatttcggc ggcacggccg gtatcgcgga gatgcttatt cagagccaca
      181 cggatgagat tgtgttgctg cccgccctcc caacagcctg gaaagacgga gccgtatcgg
      241 gtctggtcgc gcgtggggga ttcgaaatcg gcatgaagtg gcgtgatggc cggctcgaga
      301 gcgccgctgt gcactcgcga ctgggcaatc ccgggaccct tcggtacgga ccgtatacgg
      361 ccgcgctggc aggcgaaaaa ggcgaacgtg ccttgttcga cgggacactc cagccggctg
      421 ctgtccgagc gcgttgaatc ctcccggagc ctgccgtacc atttcccgtt gaaggccaat
      481 gagcagaaaa gtggcgcgca aacgcgcaag gatcgggcca tggcagcgac ttcacgggaa
      541 cgggtttatc aggccctgca gttcgaggat ccggatcgtg cgccacgcca cctgtggacc
      601 ctgccttggg cagagctgca tttccccggg gaattgagcc agctccggga ggactatccc
      661 gacgatctgg tcagtgcccc cgggttttgc caggagaccg ccagaactga cggagacgag
      721 tatgcggttg gacgctatac ggatgcgtgg ggttgtgtct tcgacaatct gcagcccggc
      781 gtcatcggtg aagtaaaaca acctctcatc agggactgga ttgccgatgc agcgaaggtg
      841 cacatcccgc gcgaatggct gaccatcgac atcgaccgcg tgaacgagtt ctgcgaagca
      901 acgcaacagt tcgtaaccgg cggctgctgt ccgcggccgt tcgaacggct gcaattcctg
      961 cgcgggaccg ccagcctata tacggacctc ttggaacctc cgccggggat ggccgggttc
     1021 gtgcgtgaaa tgcacgcgtt ctattgccaa tggatggaag tctgggcggc caccgatgtc
     1081 gatgcgctga atgtcatgga tgactggggg gcgcagcgcg cactcctaat cgatcccgtg
     1141 atctggcgcg acgtgttcaa acccctctat cgcgactacg tccatatcgc ccacggagcg
     1201 ggaaagaagg tcttcctgca cagtgacggg cacatcctcg ccatctaccc ggacctcgtg
     1261 gagcttggat ttgacgccgt caactcgcag ctcttctgca tgggagtgga gaatctggct
     1321 ccgtacgccg gcaaaaccac attctggggg gagatcgatc gccagcatct tctgcccgag
     1381 gcaaccccgg atgaaatcga ccgcgccgtc agcctggttc acaaaaccct ctgggaaaag
     1441 ggtgggtgta tcgcacagtg cgagttcggc cccggcgcaa aaccggaaaa cgtgcgacag
     1501 gttttcgctt cgtgggaccg cctcaccctt ggcggatgaa cacttcgcaa aacttgcggc
     1561 gggggagggg cttggtttac ggatttcccg ttgtgcatcc cagcatccgc ggagccctgc
     1621 ggccggccgc ctcttggaga gacaggcggc tcaggcgcgg agccgtttca ccgcgagaac
     1681 agccagatat gcggctgcgc cggtcacaat gatcatgggt ccgctcggga gatcataggg
     1741 atagcttaag gcgatacccg cggtcgagaa caccatacac aagatgaccg ccatgatcat
     1801 gatccgcgcc atgcgccgcg aaaactgccc cgccaccgca gcgggcaggg tcagcagggc
     1861 gatcacgagg acaatgccca cgagataaac caggaggacg attgtcacgg cgatcaggca
     1921 caggagtagg aggtaatagt aatcgacggc cagaccccgc gtacgcgcaa attcctcgtc
     1981 gaaacaaacc gccagcagtt tgttgtagaa aaacaccgcg acggccgtca ccaggatgtc
     2041 caggcccgcg accatccata cgtctcgctt cgtgatcagg aggatgtcgc cgaaaaggta
     2101 gctcatggtg tctttgttgt agccgggggt tttggcgagc aacaagagcc ccagggccat
     2161 gcccacagcc caaacggcgc caatgacggt gtcctcgcgc tctttggcgc gcaggctgac
     2221 gagcccgatg atgagcgccg agaataaggc cgccggcagg gcgccgtgga caggctctag
     2281 ccaggtcaat ccggtgtggg cgcgcaggta cagcacggcc ccgatgcccc ccagcacgga
     2341 atgcgcgatc gccgcagcga taaacgtgac ccggcgggtg accacaaaag agcccacaat
     2401 gccaaacgct acgctcgaca gcaggcccac cagcacggca tggcgcaaga ccggaatgtc
     2461 tggatgcagc aacgctttca gaaactcatg catgggcgtg gtccgtttca gcgtcgcgct
     2521 ggtcatgccg gatcagccgg gcatcttcac cgtacaattc gcgcaggcgc gccgtggtca
     2581 gttcgctcgt gggatgaatg tgcgcgcgct gattaacgca gatcacccgc gagaccaggt
     2641 cgtaaatgaa ggttgggtcg tgcgaaacca tgacgatggt cctgcgcttc gcgacctctc
     2701 gaagggtctg catcaggcgt ttcgctgcag caagatccac gctggccgtg ggctcgtcca
     2761 gaagcagcag gtcgggagag gaaatcaggg cgcgggcaat aagcacgcgc tggcgctggc
     2821 ctccagacag gtctgaaaat gggcggtgcc acaactcttc aatgcccatt tcgtcgagca
     2881 cgcgccgggc cgcatctttt tcctcgagag tatagcggcc gccccagtgg cgttccgcca
     2941 ggcccatcag cgtcacgtcc atcaccgaca cggggaacag ggggtcgtac tgggcgaatt
     3001 gtggcgtgta tccgacgcgc ccgcggacgg tcgcgggcgc ttgaccaaaa acccgcacct
     3061 ggccttcgtc tggagtcagg agtccgagga tgagtttgag caacgtcgac ttgccgccgc
     3121 cgttcgggcc aatcacactc gaaaacattc cggatggaat atcgagattg acatcccgca
     3181 gcacaggctg ggcgttgtac gcaaacgata cgttatggag gcataccgcc ggcgggctgg
     3241 cctcacggct ggctgcgtcc tccgcggagg taagtatcgt catggcatgc gctccgcctg
     3301 caaggcgtgg gcgaacgcgt cgccgatcgc gcgcaggtta tcgagcacgt ctgcccgcag
     3361 tggatcgagc gggacgacag tgccctgaat tgcttcggct accgatttgg cgctgcgctg
     3421 gtcgaattgc ggctgcacga agatggtatg cacgccgtct gcctgagcct gctcaatgag
     3481 ttcctgaagc tgccggggcg ttggcttttt accgtccact tccacggatt tctggcgcag
     3541 cccgtatgcc tccgcaaaat aaccgagcgc cgggtggtag atgtagaacg tgcgtcccct
     3601 gaagggtgcg agctgttctc tcaaatgatg atccaaggcg tccagaacac cctcgatcgc
     3661 cgtgagattc ctggcgtaat cgtctgcgtg cgcgggatcg atccgctgga aagtctcggc
     3721 gatcgtggcc gccatggctt tcagtatgac gggcgacatc cacacgtgcg gatccatatc
     3781 gtcggcatgg gcgtgcccgg ccgcgccctc ctcgtggtcc tgcgggctgt cctcaatacg
     3841 cgccaagtcc tcggcaatat cttggatcac cagcgacgga aagttggctt cgatccgttt
     3901 ggccagctcc agctcaaacg gaaacccgcc cacatggaaa taggccgatg cgcttccaag
     3961 cgccatcacc tgcttcggcg tggtgctgaa ggtatgcgga ccctctccag gggcgagaag
     4021 agtcgtgacg tgcacgtgat cgccaccgat cgcctccgcg agcgcggcca gcggtgggat
     4081 gctgacaaat acctcaagcg gcccgccacc tggaccaccg tgcctggccg gctcaggcgc
     4141 agggctgcat ccgcagacga atgtcaccgc tgcgaacaac acgagcggcg ttccactggg
     4201 cattaatcgt ttagcgttca tgacgccccg tggcgtccgg cgcctccccg gccatgcaca
     4261 caatgcgaag cgcatggcag tcctcacact gttccacgtt gtgcgttgtc gcaaaatggt
     4321 cccagtgttt ccgttgttcc ggcgaaagag tgcccgcacc caggcaaagc gggcacacgt
     4381 tctcaagcgc cgccaggatc gcccggcgga tgaattccga acggtttccg atgccctgca
     4441 tcgcctgcga caggccctca tcgaccttga aggtgatgat ctcctgcttc tttgctgaag
     4501 acatgcgtgc tctccgcgtt gttttcccga actataatac tttttattac caaacgcaac
     4561 gccgggttca tctattccag attgcacccg aaatccagcg cccacctccg catttttgaa
     4621 aattattcgc taatttgcgt tcacggcgct ttttgctgtt taatcaaacc aaacacgagc
     4681 aaactcagct gaagaaagca aaagtgcgcc gggcgaagct gaaatatcgt gctccctgcg
     4741 cacaaacccc gggagaacgc accatgactt cttttcgaat gatttcagtt gccttgctgg
     4801 gagttgcctt gtccttttcg atcctggctg cagagcctga gccggctgta gagcatgtgg
     4861 tcgtcattgg cgtggacggc atgacggcca aatcgctcga ggttgcaaat acccccaaca
     4921 tgcacgcgct gatggcattg ggctcataca cgcgcgcggc gcactcggtc aaaccgaccg
     4981 tgagcggccc gaactggggg tccatgctca tgggtagcaa acccgaaacc cacggcatcg
     5041 tgaataacag ctggacgctc gacgcggatg acccgtttcc aacggtcttt actgcgttgc
     5101 gcgcggcaga ccccaacgcg gtcctcgtcg cggcctacga atgggacacg tttggcaagc
     5161 tgtttgtcga tgaagtcgtg acccagcgta tcggcgtgga taaggaggct attgagaacg
     5221 atacctcaga aaacgcggcc tcccgctaca ttgccacgca ggcggcccag tgcatcaaag
     5281 agaaaaagcc caagctgacg tttatccatc tggacatggt cgaccatgcg ggccattcct
     5341 acgtctacga ttcggaaaag tataacgtgg cggttggcga agcggacgag cttgtgggca
     5401 tcgttgtaaa aggcgtcgac gacgcgggca tccggaagaa cacggccatc ttcgtcgtct
     5461 ccgaccacgg cggaatcgac aaaggtcacg gcggcgatac ccccgaagaa accatcgtgc
     5521 cgtggatcgt ggccggcgcg ggtatagtca agggcgctgc catccccgac ggcatcagtg
     5581 tcgcccagac cgggcctacc atcgcagccc tgttgggcgc cgaactgccc gcccgctgga
     5641 ccatgaagcc cgtgagccag gttttgaaaa agtaagacag ctccgggcca ccgcccgtat
     5701 gacattgccc gcgggaaatt tcctccgggc attaccacag caccctacgc ccgggacggc
     5761 tccgcacgga gcctcccggg tcgcaaataa atgcccgcag accattccag cccttgatgg
     5821 cgcgcagcca gtccaccacg cggagccctc tgttctgcca cccagccagc cacttgcccc
     5881 ccagccatta ccgcactgaa actcgccaga gccgctcggc gcccctggcc cggctgcgcg
     5941 ttctgcctgg cgtttgcata cctgtggaga tcggcgcgct ggccgatgga gacgggggag
     6001 aggtcatttc aggggggaaa atggtcgggg cggcgggatt cgaacccgcg acctcctgct
     6061 cccaaagcag gcgcgctaaa ccgggctgcg ctacgccccg actggattga ggtggccggt
     6121 agcataacat acggggcagt cgtgaagcac gcgttttgaa ccgggcgttt tgaaccgggc
     6181 gggttgcgtt cctgcgggtg gatcggccaa aatgtgtcgt ccggttggtt ttttgttttc
     6241 atggttcgcc ctgcaggacg gcgcgggatc ttgtggatgc agtcccgtgc cagcctggct
     6301 gaaggagacg ggtgcatgag gaaggtgttg acgtttatcg gggtggtggt ggtggtcggc
     6361 ggcgtggcct cgtttaatca ctggtaccgg ttgagcaatg tcatcaatct ccacaccgag
     6421 atggcgaaaa acgttcggca atcactggtc gaccggggta tgcgcccgat cagctgggaa
     6481 ttgctgaaga agacccaggg cgaatttgag tcgatccccg tgtatccgcc cgaattaaag
     6541 gaactggacg ggaagcaggt tacggtcgtg ggatacggca tgccgctggg cggcgatctg
     6601 ccgtcgcatg acgatgatca cggccacgcc cacgcgtcga tgctgccgcg cattctgccg
     6661 ctcctcgagc ttttcaaagg gcatgtccac acgcagaaat acaatggcgt gccggaggtg
     6721 atgctgacgc ctctgccgct tgaatgctat tggaaacgct tgcccccgct gaaccaggcc
     6781 ctctatgtgc gaccggccga ccccatcgaa tttgcggacg gcgcggtgct cggatttcga
     6841 ggcacgctgg agctgcggga aaaggaaggc ccgaagttct ttttcgtcct caagaacgcc
     6901 gaattgctga aataatgccg cgcgggggag gggcgtttcg ccctcggcag tatggattgg
     6961 atgctccggc accgaaggat ggggagtctc gttgaccgcc aaggcgctca ctgatccgaa
     7021 ccagccgttt gagcccggaa cttcgccgct ggaacagtcg gtggccgctt tgcccggcat
     7081 tggcgggaag cgcgctgaac tgctcggtaa tcttgatatc gcgacaatcc gcgacctgct
     7141 gttttatttc ccccgaagct atgaagatcg acgccagcct cgcccgatcg cccaagtgaa
     7201 cgaaggggat accgccacga tccgggcggt ggttgtgcgc tctaccacgc gtcgcctgcg
     7261 ccggaagctt tctgctaccg aggctctgct gcgcgatgat tccggcgaaa tgaaggccat
     7321 ctggttcggg ttgggatttc tttcgcgcac cctggtcccc ggtaaggaag gattttttac
     7381 gggtacggcg gggaagtgga acggcctgtg cctgcgaaat cccgattacg agttgctgtc
     7441 ccatgacgac gaagaccggc ttcacaccgg ccggatcgtc cccgtttacc gcctcaccga
     7501 aaaactttct cagcggatgc tgcgccggtg ggtgcgcatc gccctcgacg aggtccggga
     7561 ccacgtcgaa gaccgcctgc ctcccgacat tgccgggcgc tggcctcata ccgacgtgcg
     7621 ccgcgccatt gaggcggtcc actttcccgc caccatggag gaggcccgga aagcacgcga
     7681 acggttcgcc tacgaagaac tcctgggcat tcaactcggc atactgcagg gccggtcgcg
     7741 ccgcgcacac gaggaagtcg gctaccgcca cgttaccagc ggaccgcgcc tcaacgcgct
     7801 gcggagatcg ctcccgtttc cgttgacgca gggccaggaa cgtgccgtcg aggatatttt
     7861 ggccgatatg gcctcggccc gcccgatggt gcggctgctt cagggcgacg tgggctgcgg
     7921 aaagaccgtc gtcgcgctgc atgcgatcgc ggcggctgtg gacggcggtt tccaggcggc
     7981 cttgatggcc ccgaccgaga tcctggccga acagcatgcc ttgagcctgc gcgaatcgct
     8041 ggagccgctg ggcatgacgg tcgacgtcct gactggctca acgggcaacg cgcgtgccgt
     8101 gcgccagctc acaaccggcg gcaacgtcga cgttcttgtg ggcacccagg cgctgatcca
     8161 ggaaaaaacc ggatttcgca acctggggct ggtcattatc gacgaacagc accgctttgg
     8221 cgtggtccag cgcgatgcgc tcgtgcagaa gggcgtgcgc cccgatatcc tccacatgac
     8281 cgccacgccc at
//
LOCUS       DAOPBQ010000089         8263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8003_length_8263_cov_3.557870, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000089 DAOPBQ010000000
VERSION     DAOPBQ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8003_length_8263_cov_3.557870"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PKW60_04385"
     CDS             <1..353
                     /locus_tag="PKW60_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPJ98698.1"
                     /translation="RAASDAALALYTRARDMAASKGLILCDTKFEFGLYEGALMLADE
                     VLTPDSSRYWPASDYQPGRGQPSYDKQYVRDWLETTGWDKNSPPPALPADVVAKTREK
                     YVEAYRRLTGRDDF"
     gene            421..1884
                     /gene="purF"
                     /locus_tag="PKW60_04390"
     CDS             421..1884
                     /gene="purF"
                     /locus_tag="PKW60_04390"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPJ98699.1"
                     /translation="MSESPRHECGVFGVFQNPEATTLTYLGLYALQHRGQEGAGIVSC
                     DGNTLHAHRGVGLVADVFKAHRLASLPGDIAIGHVRYSTFGSCELKNVQPLIVDYSRG
                     SMAVCHSGNLVNAARLREELEDQGAIFHSTTDTGVILHLIARSKKKRFVDSVIDALRQ
                     VTGAYSLLATNGEELVVARDPLGFRPLWLGRLGEAVVLASETCALDIIDAEWVREVEP
                     GEVLAITRNGMESFKPFEPQQSRACIFEFIYVARPDSTLFGRSVDEVRKQLGRNLARI
                     LPVEADLVMAVPDSSNQAALGYSHESGIPFDMGFIRNHYVGRTFIEPDQRIRDFGVKI
                     KLNPARSAIQGKRIVLVDDSIVRGTTARKIIALLWKCGVKEVHFRISCPPIINSCYYG
                     IDTPRRERLLAASMTVDQMRDFIGVTSLAFNSIPSMVEATGLPTESFCLACFNNDYPT
                     PTPADFESRRNAPRHVDRSADLMGLDAGLRGAILPPV"
     gene            complement(1933..3852)
                     /gene="dxs"
                     /locus_tag="PKW60_04395"
     CDS             complement(1933..3852)
                     /gene="dxs"
                     /locus_tag="PKW60_04395"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPJ98700.1"
                     /translation="MSDTETRAGESLLKRIDAPGDLKALTVEQLKVLAKEIRDLILAN
                     ASEHGGHLAPHLGVVELTLAIHYVFDTSQDRLIWDVGHQSYTHKLVTGRRDQFHTIRC
                     KGGLSGYPRRDESPYDAFGTGHSSTSISAALGMAVARDRQKQKHKVIAVIGDGAMTGG
                     MAFEGLAHAGHLGSDLLVILNDNKMSIAPNVGALSSYLSRLITAGVYNRAREDIGSFV
                     KRMLGPQLTKAAHKLEHSVKGLITPGSFFQEFGFRYIGPADGHDLETLVGLLSNIKDF
                     RGPVLFHCVTQKGKGYPFAEEDPLKYHGVKAFDIQTGQFRGSEDSPDKTRVRSFTEAF
                     VETLIEEAHKDSRIVGITAAMPTGTGLSEFEKVFPDRVFDVGICEQHAVTFAAGLAAD
                     GQKPVCAIYSTFFQRSYDQFVHDVCLQKLPVVFALDRSGAVGEDSPTQQGAFDLSFLR
                     CIPETAVLVPRDDLDLAAMLRWSLKQPFPVVLRYARGLAPAIGAPHERDITQGEILRE
                     GREATFLAVGPILSACLEAADRLAAAGVPVGVADARSVKPLDTALLDRLADTPIITVE
                     ENSIEGGFGSAVMEYFERQGRAHDVRLTRLGFPDHFMEHATRKEQLEEVGLSADGLYR
                     AACAFLGVQVSGPVK"
     gene            complement(3959..5518)
                     /locus_tag="PKW60_04400"
     CDS             complement(3959..5518)
                     /locus_tag="PKW60_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPJ98701.1"
                     /translation="MSLNLAHFLYLTAREHPDKTAVIMDDYRFSYQDVLTNARRVASL
                     LQAKGIRRGDKVALMIPNSPHFPIIYYGILLAGAVAVPVNCLLKSHEIQYYLEDSDVR
                     LFFTWIDFLEESIKAVDATLTCQHLVVVSLPDDRKAPVAGESFNRLLEEASPDFDMVE
                     TMPDDTAVILYTSGTTGNPKGAELTHFNMFFNAYYTMHRVLYATANDVALGVLPLFHS
                     FGQTCVQNALFMAGGSMSLVPRFEAQRVLDVIQRDRVSIVAMVPTMYFWLLHEKRNGT
                     YDLSSVRMAVSGGSALPVDTMVQFEREFGVRILEGYGLSETSPVATFNIIERESKPGS
                     IGLPIWGCEMRICRNDGTFAEVGEVGEIVMRGHNVMKGYYNKQAATEEAFEGGWFHTG
                     DLARMDDEGYFYIVDRKKDLIIRSGMNVYPREVEEILYGHPAVLEAAVVGVPDPARGE
                     EVKAFVCLKSGSGVSADELQMYCRERMAKFKCPKTFEFLPELPKGPTGKILKRHLRDG
                     VISANSLSQRA"
     gene            5858..7213
                     /gene="mgtE"
                     /locus_tag="PKW60_04405"
     CDS             5858..7213
                     /gene="mgtE"
                     /locus_tag="PKW60_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPJ98702.1"
                     /translation="MPDEFTREPWEEIESIVAAQDADCLDRFLQQLNPADIARAISRL
                     DDETQAGVLTLLEPEDAADLIQELPDVQGADLLEDLPVEKAALIFDEMQSDERADLLQ
                     ELDKENAEAILAQMDPHEAANARKLLAYDADTAGGIMVTEFLAYDMNRSVADVLEDLH
                     KHVEAYSDHIIQYIYAVNEKHNLVGVIPLRNLILSRPDTGLLNVMIPNPLYVLVDSSL
                     EELAQIFDRYTLVALPVTDNNGRLVGMALRSDVEEALGEIAATALSSFSGIVGGDEFR
                     SMPVFPRALRRMSVLGFNLVLSALAASVILHFESTIGQLTALAFFIPIIGNMSGCSGN
                     QAVGVSIRELALGLIKPEDWKRVLGKEIQVGLINGALLGVFLSLVALLLHKGPLIGLV
                     AGAALALNSLIALCLGGLAPLVLRKFEIDPALAAPPIVTSLSDMCGFLIFLTVATWLL
                     M"
     gene            7323..>8263
                     /gene="trpD"
                     /locus_tag="PKW60_04410"
     CDS             7323..>8263
                     /gene="trpD"
                     /locus_tag="PKW60_04410"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPJ98703.1"
                     /translation="MLDEAIALISQRKDLTREQAASAMRILMSGLASPAQVSALLMGL
                     RVKGETIEEITGFAQVMREMAIPVAPKRRPLIDIVGTGGDHCGSFNISTSSAFVVAGA
                     GMAVAKHGNRSATSKCGSADVLEALGVNIDAAPKQAARCIDEAGIGFLFARSVHTAMR
                     HVAPIRSELRATRTVFNILGPLTNPAQPEGLSIGVFEPGLAEPLAHVLGNLGTRRAFA
                     VSGKDGLDEISLSGPTWIAELRNGHVSTYEITPEQVGLKTVPRGALAGGSPAENARIL
                     RNVLSGEHGPHRDVVLLNAASGLLTGDDSLDWFSALDP"
BASE COUNT         1669 a         2443 c         2469 g         1682 t
ORIGIN      
        1 caagagcggc atccgacgct gccctggcgc tctatacccg cgcccgggac atggctgcct
       61 ccaaagggct tattctgtgt gacacgaaat ttgagttcgg cctgtacgaa ggcgcattga
      121 tgctggccga cgaggtgctg actcccgact catcacgcta ctggcccgcc agcgactatc
      181 aaccgggccg ggggcagccc agttacgaca aacagtatgt gcgcgattgg ctcgaaacga
      241 cgggctggga caaaaattca ccgccgccgg cattgcccgc agacgtggtc gcgaagaccc
      301 gtgaaaagta tgtggaagcc taccggcggc tgacgggcag ggacgatttc tgagaccatg
      361 cagcatggcg gccaagaaga acccctcggc gcatcctggg acagggggga ggacacggag
      421 ttgtcggaat cgccgcggca cgagtgcggc gtttttgggg tatttcaaaa ccctgaagcc
      481 accacgctca cgtatctggg gttgtatgca ctgcagcacc gcggccagga aggcgcggga
      541 attgtcagtt gcgatggcaa caccctgcac gctcatcgcg gcgtgggact tgtcgcggat
      601 gtgttcaagg cgcaccgcct ggccagcctg cccggagaca tcgccatcgg ccacgtgcgc
      661 tattcgacct ttggcagctg cgaactcaag aacgtccaac cgctgatcgt tgattattcc
      721 cgggggtcaa tggctgtctg ccacagcggg aacctcgtga atgccgcccg gctccgcgaa
      781 gagctggaag accagggcgc cattttccac tccaccacgg acacgggcgt tattctgcac
      841 ctgatcgccc gctctaaaaa gaagcgtttc gtggatagcg tcatcgatgc attgagacag
      901 gtgaccggcg cctattcgct gttggctacc aacggcgagg agctggtggt ggcgcgggat
      961 ccgctgggat ttcgcccgct ctggctgggc cgtctgggtg aggccgtcgt gctggcgagt
     1021 gagacctgcg cgctggatat catcgacgcg gaatgggtgc gcgaggtaga accgggtgag
     1081 gtgcttgcca ttacgcgaaa tggcatggag tcgttcaaac ccttcgagcc acagcagtcc
     1141 cgggcctgca tcttcgagtt tatttatgtg gcccggccgg acagcacgct ttttggccgg
     1201 agcgtggatg aagtgcgaaa gcagctgggg cgtaatctgg cgagaatcct cccggttgag
     1261 gccgatctcg tcatggcggt gccggactcc tccaaccagg ctgccctggg ctattcacac
     1321 gaatccggga tccccttcga tatgggattc atccgaaacc attatgttgg ccgcacgttc
     1381 atcgagccgg atcagcgcat ccgcgatttt ggcgtgaaga tcaagcttaa tcccgcgcga
     1441 agtgccatcc agggaaagcg cattgtgctc gttgacgact cgattgtgcg cggtaccacg
     1501 gcgcgcaaaa tcattgcact cctctggaaa tgcggtgtga aagaggtgca tttccggatc
     1561 agttgcccgc ccatcatcaa ttcatgctac tacggaatcg ataccccccg gcgggaaagg
     1621 ctgctcgccg cctccatgac ggtcgaccag atgcgcgact tcatcggagt cacttctctg
     1681 gctttcaaca gcatccccag catggtggag gccactggac tccccacgga atccttctgc
     1741 ctggcctgct tcaacaacga ctatccgacg ccgacacccg cagattttga atcaagacga
     1801 aatgcgccgc gccacgtgga tcgcagcgcc gatctgatgg gtctggatgc cggactgcgg
     1861 ggggcgatcc tgcctcctgt ctgaccggcg gaaaggcacg cgcgggcggc gggagctctg
     1921 cagccgccgc acctatttaa cgggtccgga tacctgtacg ccgagaaatg cgcaggcagc
     1981 tcgatagagt ccgtccgcgc tcaagcccac ttcttccagc tgttccttgc gcgttgcgtg
     2041 ttccatgaaa tgatcaggaa aaccaaggcg ggtcaagcgg acgtcatggg cgcggccctg
     2101 acgttcgaag tactccataa cggcggagcc aaacccgcct tcaatggagt tctcttctac
     2161 cgtgatgatg ggagtatcgg ccagtctgtc gagcagagcg gtatccagcg gcttgaccga
     2221 acgagcgtcg gcgactccaa ccggcacacc ggcggcagcc agcctgtcgg cagcctcgag
     2281 acaggcgctc agaatgggtc cgaccgccag gaaagtggct tctctgccct cccggaggat
     2341 ttcgccctga gtgatgtccc gttcgtgcgg cgcgccaatg gcgggcgcca gcccgcgcgc
     2401 atagcgtaac accacgggaa acggctgttt gagcgaccag cgcagcatcg cggcgagatc
     2461 caggtcgtca cgcggcacca ggacggccgt ttcgggaatg caccgcagaa acgataaatc
     2521 gaaggcgccc tgctgcgtgg ggctgtcttc tcccaccgca ccggagcgat cgagcgcgaa
     2581 cacaacgggg agcttctgca ggcatacatc gtgcacaaac tggtcgtaag aacgttggaa
     2641 aaaggtcgaa tagatggcac agacgggttt ttgtccgtcg gcggccagtc ccgcggcaaa
     2701 cgtcaccgca tgctgttcac agattcccac atcgaacacg cggtcgggaa aaactttttc
     2761 gaattcactc aagccggttc ccgtgggcat ggcggccgta atgccgacga ttcggctgtc
     2821 cttgtgcgcc tcttcaataa gggtctcaac aaacgcctca gtaaaagagc ggaccctggt
     2881 cttgtcgggg ctgtcttcgg aaccgcggaa ctggccggtc tggatatcga aggccttcac
     2941 gccatgatat ttcagcgggt cttcttcggc aaaaggatac ccttttcctt tctgggttac
     3001 gcaatgaaag aggacggggc cgcggaagtc tttgatgttc gacaacagcc ccaccaaagt
     3061 ctcaaggtcg tggccgtcgg ccgggccgat gtagcgaaac ccgaattcct gaaagaaaga
     3121 ccccggcgtg atcaggcctt tcacggaatg ctcgagcttg tgcgcggctt tcgtgagctg
     3181 aggccccagc atacgcttca caaacgagcc gatatcttcg cgggcgcggt tgtacacccc
     3241 cgcggtaatc aagcggctca ggtacgacga gagggcgccg acattcgggg cgatcgacat
     3301 cttgttgtcg ttgaggatca cgagcaggtc agagcccaga tgtccggcgt gtgccaatcc
     3361 ttcgaaagcc atgccgccgg tcatggcgcc gtcgccgatt accgcgatca ccttgtgttt
     3421 ttgtttttgc cggtcgcgcg ccacggccat gcccagcgcc gccgagatcg acgttgagct
     3481 gtggccggtg ccgaacgcat cgtacgggct ttcatcccgg cgcggatatc cgctgagccc
     3541 gcccttgcag cgaatggtgt gaaactggtc gcggcgcccg gtcaccagct tgtgtgtgta
     3601 cgattggtgc cctacgtccc aaatcaggcg atcctggctg gtgtcaaaaa cataatgaat
     3661 ggccagcgtg agttcgacca cccccaggtg gggcgcaaga tgacctccgt gttctgaggc
     3721 attggccaga ataaggtccc ggatttcctt cgccagaacc ttcaactgct ccacagtgag
     3781 ggctttcaaa tccccgggag catcgattcg tttcaacagg ctctcgccag cgcgagtctc
     3841 ggtgtcactc attctgaaat cccgcaagtt aacagcttta gacagcgtcc tcgagtatag
     3901 cagaggaaag gagcgggccg catccccgga cgcttgaaaa ccctgcaatc acgccccgtc
     3961 acgcgcgctg agacagggaa tttgccgaaa taacgccgtc gcgcagatgg cgtttgagta
     4021 ttttgcccgt aggtcctttg ggaagttcgg gaagaaattc aaacgttttg gggcatttga
     4081 acttagccat tcgttcccgg caatacatct gcagttcgtc cgcggatacc ccagagccgc
     4141 ttttgaggca gacgaatgct ttgacctctt cgccgcgcgc cgggtcaggc actcccacga
     4201 cggccgcttc cagcaccgcc ggatggccgt agagaatttc ctccacttcc cgtggataga
     4261 cgttcatgcc gctgcggata atgagatctt tcttgcgatc gactatatag aaataaccct
     4321 cgtcgtccat gcgggccagg tcgccggtat gaaaccagcc gccttcaaag gcttcttcgg
     4381 tggcggcctg cttgttgtaa taccctttca tcacattgtg gccccgcatg acaatttcgc
     4441 cgacttctcc cacctcagca aaggtgccgt cgtttcggca gatacgcatt tcgcagcccc
     4501 agatgggcag gccaatcgat cccggtttgc tctcgcgttc gatgatatta aacgtcgcca
     4561 ccgggctggt ttctgacagg ccatagccct caaggatgcg gacgccgaac tcgcgttcga
     4621 actggaccat ggtgtcaacg ggcagcgcag agcccccaga gaccgccatc cgaaccgagg
     4681 agagatcata cgtaccgttg cgtttttcgt gcagaagcca gaaatacatg gtcggcacca
     4741 tcgcaacgat gctgacgcgg tcgcgctgaa tcacgtcgag cacgcgttga gcctcaaagc
     4801 ggggaaccag gctcatcgag cctccggcca tgaagagggc gttttgcacg caggtttgcc
     4861 caaacgaatg aaacagcggg agcaccccca gcgcgacgtc gtttgccgtc gcatacagaa
     4921 cccggtgcat ggtgtaatag gcgttaaaaa acatattgaa atgcgtaagt tccgcgcctt
     4981 tcggattgcc cgtggtgccc gaggtgtaga gaatgacggc tgtgtcatcg ggcatggtct
     5041 ccaccatgtc aaaatcgggc gaagcttcct caagcagccg gttgaacgat tcgcctgcga
     5101 ccggggcctt gcggtcatcg ggaagactga ccaccacaag atgctggcag gtcagggtcg
     5161 catcaacggc cttgatcgat tcttcaagaa agtcgatcca cgtgaagaac agccgcacat
     5221 ccgagtcttc gagatagtac tgaatttcat gcgacttgag gaggcagttc acgggaacag
     5281 ctacggcgcc ggccagcaga atgccgtaat aaatgatggg gaaatggggt gaattgggaa
     5341 tcatcagagc gactttgtcg ccacgccgaa tgcctttggc ttgaagcagg ctggcaacgc
     5401 gccgggcatt tgtgagcaca tcctgataag aaaaacggta gtcgtccata atgaccgcgg
     5461 ttttgtcggg gtgttcccgg gcggtcagat acagaaaatg ggcaagattc aaactcatca
     5521 ggaacccccg ttgttggtgg ttgtgtgatt tccggatagc cggtgtgtgc ccttgtattg
     5581 cacgatactc cgtcgcacca cgccgctatg cccggttgcc gttttcccgc cgccgatcct
     5641 gggtcggctt aacggggtcg acagctcccc ggggcacggt ttcccggcgt caggcccttt
     5701 ttggtgccga cagctaagtt gtgataatat cacgttttaa atgtgtcgcc aaattgacgg
     5761 ccgccaagat tccacgcggg tgcggcaggc cggtttttgc tcgcctttgt ggcgtttccg
     5821 taccctggtc catgcctgtc acgagaggag tccggttatg cccgacgagt tcacacgcga
     5881 accgtgggaa gaaattgaga gcatcgtagc tgcacaggac gccgactgtc tcgatcgttt
     5941 tctccagcag ctgaatccgg cagatatcgc ccgagccatt tcccgtctcg atgatgagac
     6001 ccaagcgggg gttctcaccc tgctcgagcc ggaagatgcc gccgacctca ttcaagagtt
     6061 gcccgatgtg cagggcgcgg atctcctgga agacctcccg gtcgaaaaag cggcgctcat
     6121 tttcgatgaa atgcagagcg atgagcgcgc cgaccttttg caggaactgg acaaggaaaa
     6181 cgcggaggcc atcctggcgc aaatggatcc gcacgaagcc gccaacgcgc ggaagttgct
     6241 cgcctatgat gccgacaccg cgggcggcat catggtcacg gaattccttg cctacgacat
     6301 gaacaggagc gtcgccgacg ttctggaaga cctgcacaag catgtggagg cgtattccga
     6361 ccacatcata cagtacattt acgcggttaa tgagaaacac aacctcgtgg gagtgatccc
     6421 tctgaggaac ctgatcctgt ccaggccgga cacaggcctg ctcaacgtga tgatccccaa
     6481 tccgctgtac gtgctggtcg attcatccct cgaagagctc gcccagatct tcgatcggta
     6541 caccctcgtg gcgttgcccg ttactgacaa caacgggcgc ctggtgggaa tggcgcttcg
     6601 ctcggatgtc gaggaagcgc ttggcgagat tgccgcgacg gccctcagca gcttcagcgg
     6661 tattgtgggc ggcgacgagt tccggtccat gccggtgttc ccccgagcct tgcggcgaat
     6721 gtccgtgctc ggttttaatc tcgtgctgag cgccctggca gccagtgtga ttctccattt
     6781 tgaatcgacc atcggccagc tgacggcctt ggcgttcttc atccccatta tcggcaacat
     6841 gagcggctgc tccggaaatc aggcggtcgg agtcagcatc cgggagttgg ccctgggtct
     6901 gatcaaaccc gaagactgga aacgggtgtt ggggaaagaa attcaggtgg ggctgattaa
     6961 tggagcgctg ctgggcgtgt ttctctcgtt ggtagcgctg ctgctccata aaggcccgct
     7021 gatcggcctt gtagccggcg cagccctggc cttgaacagc ctgatcgcac tgtgtctcgg
     7081 aggacttgcc ccgctggtcc tgcgcaaatt tgaaattgac ccggcgctgg cggcgccgcc
     7141 cattgtgacc agcttgtcgg atatgtgtgg gttcctgatt ttcctcacgg tggccacgtg
     7201 gctgctcatg tagcgcctgc ggggcattgt gttgacttta ttttcagccc gttgttactg
     7261 ttgctccgac ggcggcggca ctgcggagca tacacccagg gagaaggtag cgggttccag
     7321 ccatgcttga cgaggctatc gctttaatca gccagcgcaa agaccttacg agagaacagg
     7381 ccgcgagcgc catgcgtatc ctcatgtctg gcctggccag tccggcccag gtttccgccc
     7441 tgctcatggg gctccgggta aagggcgaga ccatcgagga aattacagga ttcgcccagg
     7501 tcatgcggga aatggcgatt cctgtcgccc cgaaacggag accgttaatc gatatcgtcg
     7561 gcacgggcgg cgaccattgc ggcagcttca atatttcaac ctcctctgcc tttgtagtgg
     7621 ctggcgctgg aatggccgta gctaaacacg gaaaccgcag tgccaccagc aaatgcggca
     7681 gtgcagacgt cctggaagcc ctgggcgtca atatcgacgc cgctccgaag caggcagccc
     7741 gctgtattga cgaggccggc atcggattcc tctttgcccg ttcggtgcac acggccatgc
     7801 gccacgtggc gcctatccgc tcggaattgc gggccacacg gaccgtattc aacattcttg
     7861 gcccgctcac caacccggcg cagccggaag ggctttcgat cggcgtgttt gaaccgggtc
     7921 tcgccgaacc cctggcccat gtgctgggca acctcggcac ccggcgcgcc tttgccgttt
     7981 ctggcaagga cggcctggac gagatctcgt tgtctgggcc tacctggatt gccgaacttc
     8041 gaaacgggca cgtgagcaca tacgaaatca cacccgagca ggtgggtctg aaaaccgtgc
     8101 cgcgcggcgc cctcgcaggc ggctctcccg ctgaaaatgc caggatcctg cgcaacgtcc
     8161 tgagcggcga gcacggaccc cacagggatg tggtgcttct caacgccgca tcaggcctgc
     8221 tgactggcga cgactcgctt gactggtttt ccgcactgga ccc
//
LOCUS       DAOPBQ010000090         8218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8046_length_8218_cov_4.077790, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000090 DAOPBQ010000000
VERSION     DAOPBQ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8046_length_8218_cov_4.077790"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PKW60_04415"
     CDS             complement(<1..726)
                     /locus_tag="PKW60_04415"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HPJ98704.1"
                     /translation="MAEVLKGKVLVAQGGGPTAVINQSMVGAVLESRKFPQVTRVYGA
                     VHGVRGIIDEDFLDLTEATTHNLEAVAQTPSSGLSSTRDKPDIEYCRKIFQVCQAHEV
                     RYFFYIGGNDSADTCRIVNEESDKAGYELRVIHIPKTIDNDLKVTDHCPGYGSAAKFV
                     AQAFAGANLDNRAIPGVYIGIVMGRHAGFLTAASILGKKYPDDGPHLIYLPERPFNKK
                     QFVQDVQNVYSKYGRCIIAASEGI"
     gene            982..2280
                     /locus_tag="PKW60_04420"
     CDS             982..2280
                     /locus_tag="PKW60_04420"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98705.1"
                     /translation="MSVNLSRRKFLSRAAAAIAAPYIIPSSALGLDGAVPPSERIVVG
                     CLGIRGRGVHDLRWMIGNPDVQVVAICDLQKTQREGVKALIDQRYGNTDCAMYRDLRE
                     FLPSRPDIDAILIATGDRWHAQAAVLAMREGKDVFSEKPSCMTITEGRAVVETAERYG
                     RVYQTGTQRLSEANHVFAIEMARTGRLGKVDVAYAHIAPWDAAKMPREWMEAQPEPPK
                     EEVDWEIWLGPCPWRPYNEEYARGGWRGDYDFHTSCIGEWGAHTFAQAQAGLDLGESS
                     PIKYNYVDNDSGDGMVCEFATGQKLIFSRGDKYWYGSCGERFDGERGWAAAADGYSEP
                     DVSSPELLREFDEVVGEYVARTGRALDHMRNFLDCVKSREEPVANARVMHHSMCTVHA
                     ANICMWLERDLEFDPATETFVNDDEANRLRSRAYREPWVI"
     gene            2313..5021
                     /locus_tag="PKW60_04425"
     CDS             2313..5021
                     /locus_tag="PKW60_04425"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPJ98706.1"
                     /translation="MRNAVLSFTLAGMLVLSAALPALAQDANPLAVLQSDADLKAKQD
                     ACIALSIYGGPEAIPVLEGLLTNAELSHMARYALEPMPYPEAGAALRNALAATSGDLK
                     AGIMNSLANRRDTECVPALIAALSDANATVAKEAARALGALATPECAAALVNALPQAD
                     AASGRLHAVCDGMLRCAEAFLASGNRAEAVALNDRLRETPNVAHEVRTGALRGAVLAR
                     GPQEGLPILLEAIRSEDKASFNGALRTAREMDGGDAVTAALVESLPALPNERKIALMQ
                     TLGHRGGSVAGPALLAEAQAGPENVRIEAVNALTRLAYTPALQLIAELATADEDVPLT
                     QAARNALAYFPAPDADAVLVAMLDNPEPAARRVAVELIGRGGLPKPADVLMNAVEKDA
                     NTAVRLSALTALEKSAGIPEIPRLLAVLRKAQAPEEIQGAEDTLRAVVNRERKPTSAG
                     IVIQEAVYGDLPGGASANVTDKVAHLVASGAMAIGASNGNFGDPAPSIVKQLRIDYEV
                     NGVSHSKTVTEGQSLNLTAFSIPPAIVDAFREAFEETRRSSPQTALALVRLLGATGSP
                     DALETVRLAAAQGEGEIKQTSERTLCDWPTSDALPVVMDLVRTTPDATLKVVALRGAV
                     RMLKESDSTPAERLEQLTELMSLAATPDEKKQVLSGLAATPSLDALEMAFAQFADDAI
                     RPEAVQAAIAIAQHLGTSAREDAAIFNGRDLTGWIPTGNFWRVEDGAIVGQSTEQVPD
                     TCYLWSELEAGDFYLSAEVLLEPPTANSGIQFRSKKIDDAGHALGYQGDIGKDVWGRL
                     YHQGGRGMLDWNGRAEEAVKPGEWNRYEILAVGPAIWTAINGKLGVACLDLTATDERT
                     GLFAFQMHAGAPQTHRYRNLKLVRDPKIELGELTADVLIPELTIPE"
     gene            5228..7543
                     /locus_tag="PKW60_04430"
     CDS             5228..7543
                     /locus_tag="PKW60_04430"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HPJ98707.1"
                     /translation="MSRTGIVALCFLLAVAGAEAIELDQSPTATGEWGYRPTPESVPV
                     TNPPGFTWRPCKDAAAYHLQVAPDEAFGTLAYEKPDIRWSAHCPPSTFSPGVYHWRYA
                     AIDATGQRTEWSTVRSFTVSPEAVAFPLPSTAELIQRMPGDHPRLFFRPEELPRLREL
                     AEGPLQEPFDALVRAADELLANPPDTSEPPLYPEGTVRLSNEWRKIWWGNRVHTQKLT
                     DGAATLAFVYRMTGDERYGRAARDLLLAFAQWDPKGSTNYRYNDEAAMPGLYYPSRTF
                     SWVYPLLTDAERSTIADVMKIRGQDCFDSLQHSPHLWRPYNSHHNRAWHWLGEVAIAF
                     YGEFPEAEPWLEYAMTILCTTYPVWNDADGGWHEGLAYWSSYLMRFTYWADVVRAAFD
                     IDVYDVPFFSNVGNYGMYLMPPGTVHGGFGDQTTTMKSSSIASLMAVFAVGAQNPYWQ
                     WYADRHQADVGGGYIGFLRRARGTKLEATPPAELPSSIQFPGVGLAVLNSNLLGAADN
                     VQIHFKSSPFFGRQSHGYNANNAFLLNVGGQPVFVRSGRRDVHGSAHHKEWMWHSRSD
                     NAILVNGESQIKHSADARGELIAFHTSPALDVIAGEASASYEHLDLWKRRIIFFKPHG
                     FLIHDILRAPEPSQFQWTLHTKGAMNIEGNRVWWEGAPGRVDVDFAYPAGLVITQTDQ
                     YEPPPAEWTGWNLGEWHLSAETPDRASQQEFVTFVAVQGAAAAIDAAHSALPRNISVA
                     LPGGTATVALSDAAFTVTLPGAEPQTFAESR"
     gene            complement(7574..>8218)
                     /locus_tag="PKW60_04435"
     CDS             complement(7574..>8218)
                     /locus_tag="PKW60_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPJ98708.1"
                     /translation="GMVAGLTTSYADTLRPALQIIGARPDVSRVAGMTMLAIRDHVYC
                     FADVSVTIEPTDEELADIAILCADTVRDFDLEPRIAMVSFSNFGSAQAPEAEKMRRAV
                     ERVRERAPGLLIDGEMQADIAISPELLRESFPFSNLPGGANILIFPCLNSSNAGFKLA
                     QKFSGAEVIGPILLGMNKPVHIMQYGGFNEADVVNMTALAVVEAQQALGGLLRK"
BASE COUNT         1565 a         2618 c         2565 g         1470 t
ORIGIN      
        1 gatgccttcc gacgcggcaa tgatgcagcg gccgtatttc gagtagacgt tttgcacatc
       61 ctgtacgaac tgcttcttat tgaagggacg ttcggggagg tagatcaggt gcggaccatc
      121 gtccggatat tttttaccga gaatcgacgc ggccgtgagg aaacccgcat ggcgtcccat
      181 gacgatgccg atgtagacgc ccgggatcgc gcggttgtcg aggttggcgc ccgcgaatgc
      241 ctgcgccaca aacttcgccg ccgaaccgta gccggggcag tggtccgtca ctttgagatc
      301 gttgtcgatg gtcttgggaa tgtggatgac gcgcaattcg tagccggcct tgtcggattc
      361 ttcattgacg atgcggcagg tgtcggcgct gtcgttgccg ccgatataga aaaaataccg
      421 gacttcgtgg gcctggcaga cctggaagat cttgcggcag tattcaatgt cgggtttgtc
      481 gcgggttgac gacaggccgg acgacggcgt ctgtgcgacc gcttcgagat tgtgggtggt
      541 cgcctcggtg agatcgagaa aatcttcgtc gatgataccg cggacgccgt ggacggcgcc
      601 gtacacacgc gtgacctgcg gaaacttccg ggattcgagc accgcgccga ccatgctctg
      661 attaatcacg gctgtcgggc cgccgccctg cgctaccaat accttgcctt tcagcacttc
      721 cgccatggca cttctcctcc agcatgaacg atggttgtct gatttccgaa ttcgcgcggc
      781 actgttggcg atgcgaggga cggagtgtct gcatcctcgc gcgtgaacgc aaagtataca
      841 aaatgtgggg cgcgtgtatc catttttcaa agggaccgcg ttcttgacgc gggcggggaa
      901 gaatcgtcac aatcacacga tatgtgtccc ggcgcgcgaa ctcgcacaag gaacacaacg
      961 cagcatcagg agggcttacg aatgagtgtc aatctgtccc gccgaaaatt tctgtcccgg
     1021 gcggccgcgg ccatcgcggc accatacatc ataccctctt cagccttggg tctggatggg
     1081 gcggtacctc cgtcagagcg cattgttgtg gggtgcctgg ggatccgtgg ccgtggcgtg
     1141 catgatctgc gctggatgat tggcaacccc gacgtgcagg tggttgcgat ctgcgatttg
     1201 cagaagaccc agcgcgaagg ggtcaaggca ttgatcgatc agcgttacgg taataccgac
     1261 tgcgcgatgt accgcgatct ccgcgaattc ctgccgtccc ggccggatat cgacgcaatc
     1321 ctgatcgcca cgggcgaccg gtggcacgcg caggcggccg tcctggcgat gcgcgaagga
     1381 aaagacgttt tctccgaaaa accttcgtgc atgaccatca ccgaagggcg ggccgtcgtg
     1441 gaaaccgcgg agcgttatgg ccgggtctac cagaccggca cccagcggct cagcgaggcc
     1501 aaccacgtgt tcgcgatcga aatggcccgt accgggcgtc tgggtaaagt ggatgttgcg
     1561 tacgcgcaca tcgccccctg ggacgccgcc aagatgcccc gcgaatggat ggaagcccaa
     1621 ccggaacctc cgaaagagga ggtggactgg gaaatctggc tcggcccctg cccgtggcgc
     1681 ccgtataacg aagaatacgc ccgcggcgga tggcgcggcg attacgattt ccataccagc
     1741 tgcatcggcg aatggggcgc gcacacgttt gcgcaggccc aggccggtct cgatctgggc
     1801 gaatcgtcgc ctatcaaata caactacgtg gacaacgatt ccggcgacgg catggtctgc
     1861 gagttcgcca cgggccagaa gctgatcttc tcgcgcggcg acaaatactg gtacggttcc
     1921 tgcggcgaac gcttcgacgg ggagcgcggc tgggccgcgg ccgccgacgg ctattcggag
     1981 cccgacgtct cgtcgcccga gctgctgcgc gaattcgatg aggtggtagg cgaatacgtc
     2041 gcccgcacgg gccgcgccct ggaccacatg cgcaatttcc tcgattgcgt caagtcgcgc
     2101 gaggaaccgg tggccaacgc ccgggtcatg caccattcca tgtgtacagt acacgctgcg
     2161 aacatctgca tgtggctcga acgcgatctt gagttcgacc cggccaccga gacgttcgtg
     2221 aacgatgacg aagcgaaccg tttgcgttcg cgcgcctacc gggagccgtg ggtgatatga
     2281 ggcataggat gcaaaaagga gcacacgcaa acatgcggaa cgccgtactc agtttcacac
     2341 tggcggggat gcttgtcctc agcgccgccc tgcccgcgct ggcgcaggat gcgaatcctc
     2401 tggccgtttt acagtctgac gccgatctca aagccaaaca agacgcctgc attgcactca
     2461 gcatctacgg cgggcccgag gccatccccg tgcttgaagg cctgctgacc aatgcggagc
     2521 tgtcgcacat ggcgcgctac gcgctcgaac ccatgccgta tcctgaagcc ggagcggcgt
     2581 tgcgcaatgc attggctgcc accagtggcg acctcaaggc aggcatcatg aattcgctgg
     2641 caaaccgccg cgataccgaa tgcgtgccag cgttgatcgc cgcattgtcc gatgccaacg
     2701 ccaccgttgc gaaggaagcc gcgcgcgctc tgggtgctct cgctacgccc gaatgtgcgg
     2761 ccgccctggt caacgcgctt cctcaggccg atgccgcatc cggccgtctc catgccgtct
     2821 gcgacggcat gctgcgctgc gccgaggcgt ttctcgccag cggcaaccgt gcggaagccg
     2881 ttgcgttgaa tgaccgcctg cgcgagacgc ccaacgtcgc ccacgaagtg cgcacgggtg
     2941 cgctgcgtgg cgcggtgctc gcccgcgggc ctcaggaagg cctgcccata ctgctcgaag
     3001 cgatacgcag cgaggacaag gcatctttca acggcgcgct ccgcaccgca cgcgagatgg
     3061 acggaggcga cgccgtgacc gccgccctgg tggagtcttt gccggccctg cccaatgaac
     3121 gcaaaatcgc gcttatgcag acgcttggcc accgcggcgg aagcgtggcc ggtcccgcgc
     3181 tgctggccga agcccaggcg ggccccgaga acgtccgtat cgaggcggtc aatgccctga
     3241 cccggctggc ctataccccg gcactgcagc tcatcgctga gcttgccacg gccgatgaag
     3301 acgtcccgtt gacccaggcc gcacgcaacg ccctggctta tttcccggcc ccggacgccg
     3361 atgcggtgct cgtggccatg cttgacaatc ccgagcccgc cgcgcggcgt gtagcggtcg
     3421 aattgatcgg ccggggcggt ctgccgaagc ctgcggacgt gctgatgaat gccgtcgaaa
     3481 aagacgcgaa taccgccgtg cgcctgagcg ccctgacggc tctcgagaag tctgccggca
     3541 tccccgagat cccgcggctg ctcgccgtgc tccgtaaagc ccaggctccc gaggaaatcc
     3601 agggggcgga ggacacgctg cgggccgttg tgaaccggga acgtaaaccc accagcgccg
     3661 gcatcgtgat tcaagaggcg gtttacggcg acctgcccgg cggcgcgtcc gcgaatgtaa
     3721 ccgacaaggt ggcccacctg gtcgcctcgg gcgcaatggc cattggcgcg tccaatggca
     3781 atttcggcga tccggcaccg agcattgtga agcagttgcg cattgattac gaggtcaatg
     3841 gtgtgtctca cagcaagacg gtcaccgagg ggcagtcgct caacctgacg gccttctcga
     3901 tacctcccgc catcgtggat gcgttccgtg aagcgttcga agagacccgc cgcagttctc
     3961 cgcagaccgc cctggcactg gtgcgcctgc tgggcgccac gggcagccct gacgccctcg
     4021 agacggtccg gctcgctgcc gcgcaagggg aaggcgagat caagcagacc agtgaacgca
     4081 ccctgtgtga ctggcccact tccgatgcgc ttccagtcgt catggatctg gtccgaacca
     4141 caccggatgc caccttgaag gtcgtggctc tgcgcggcgc cgtgcggatg ctcaaagaaa
     4201 gcgacagcac gccggcggaa cggcttgaac agctcaccga gctcatgagc ctcgcagcca
     4261 cgcccgacga gaagaaacag gtgctgagcg gtctcgcagc cacgcccagc ctcgacgcgc
     4321 ttgaaatggc gttcgcacaa tttgccgacg acgccatccg gccggaagcg gtgcaggccg
     4381 ccatcgcgat cgcgcagcac ctcggcacgt ccgcgcggga agacgcggcc attttcaatg
     4441 gccgggatct caccggctgg atccccacgg gcaatttctg gcgtgttgaa gacggtgcga
     4501 tcgtgggcca gagcaccgaa caggtccccg acacctgcta cctctggtca gagctcgagg
     4561 ccggcgactt ctacctcagc gcagaagtcc tgctcgaacc gcccacggcc aactccggca
     4621 ttcagttccg ttcgaaaaag atcgacgacg cggggcacgc gctcggatat cagggcgaca
     4681 tcggcaagga cgtctggggc cgcctctacc atcagggcgg ccgcggcatg ctcgactgga
     4741 acggccgcgc ggaagaggcc gttaaacccg gcgagtggaa ccgctatgaa atcctggccg
     4801 tcgggcccgc catctggacg gccatcaacg gcaaactggg cgtggcctgc cttgatctga
     4861 ctgcaacgga cgaacggacc ggcttgtttg cgttccagat gcacgcgggc gcgcctcaga
     4921 cgcaccgcta tcgcaatctc aagctcgtcc gcgacccgaa aatcgagctg ggcgaactga
     4981 cggcagacgt tctgatccct gaactgacca ttcccgaatg agtcgacccc ttccggcgcc
     5041 gccagcctgt acggctcgcg gcgccttttt tttgagccca ggtatggctt caccgctgcc
     5101 tcatgctcaa aagccgtatt ctcgcgatgg atccgctcaa gaactgcgaa cggtaaatct
     5161 ggtatggtga gaaaaattcg caggcacaac cgccgaggcg gtttgtgcag gaatgtggag
     5221 gaacaccatg tctcgtaccg gcatcgttgc gttgtgtttt ctgctggctg tcgcgggcgc
     5281 agaggccatt gaactcgatc agagccccac ggctacggga gaatggggct atcggcccac
     5341 gccggagagc gtgcccgtga ccaatcctcc cgggtttacc tggcgcccct gcaaagacgc
     5401 cgccgcctat cacctgcagg tggcgcccga tgaggctttc ggaacccttg cgtacgaaaa
     5461 gcccgatatc cggtggtcag cacactgccc cccgagcacc ttctcgccgg gcgtctacca
     5521 ttggcgttat gcggcgatcg acgcaacggg tcaacgcacg gaatggagca ccgtgcgctc
     5581 gttcaccgtg agccccgaag ccgtcgcatt tccactgccg tccaccgctg aactgattca
     5641 gcgcatgccc ggggaccatc cccgtctgtt ttttcgccct gaggagcttc cccggcttcg
     5701 cgaactcgca gagggcccgc tgcaagagcc tttcgacgcg ctggtgcgcg ctgcagacga
     5761 acttctggcc aatccgcccg acaccagcga gccgccactg tatccggaag gcacggtacg
     5821 cctcagcaac gaatggcgga aaatctggtg gggcaaccgg gtgcacactc agaagctgac
     5881 cgacggcgcg gccaccctgg cctttgtgta ccgcatgacc ggcgatgagc gttatggacg
     5941 ggccgcccgc gacctcctgc tggccttcgc gcaatgggat cccaagggct cgaccaacta
     6001 ccgctacaac gacgaagcgg ccatgccggg actctactac ccctcgcgca ccttttcctg
     6061 ggtatacccc cttttgaccg atgcagagcg cagcaccatc gccgatgtca tgaaaattcg
     6121 cggacaggac tgttttgaca gccttcaaca cagtccgcat ttgtggcggc catacaacag
     6181 ccaccacaac cgggcctggc actggctggg cgaggtggcc atcgcgtttt acggtgaatt
     6241 tcccgaagcg gagccctggc tcgaatacgc catgaccatc ctgtgcacaa cgtacccggt
     6301 gtggaacgac gccgacggcg gctggcatga aggcctggcc tactggtcga gctacctgat
     6361 gcggtttacg tactgggccg atgttgtccg tgccgctttc gacatagacg tgtacgacgt
     6421 gccgtttttc agcaatgtcg gcaattacgg gatgtatctg atgcctcccg gcacggtgca
     6481 cggcggcttc ggcgatcaga ccaccaccat gaaatccagc agcatcgcct ccctgatggc
     6541 ggttttcgcc gtgggcgcac agaatcctta ctggcaatgg tatgcggaca ggcaccaggc
     6601 ggatgttggg ggcggctata tcggcttcct gcgccgggct cggggcacga aactcgaggc
     6661 aacgccgccc gcggagcttc cctcatccat ccagtttccc ggggtggggc tggccgtgct
     6721 caacagcaac ctgcttggcg ccgcggacaa cgtgcagatc cacttcaaga gcagcccgtt
     6781 ctttggccgg cagagccacg gatacaacgc aaacaacgcg tttctgctca atgttggcgg
     6841 gcagccggta tttgtacgca gtggacggcg cgacgtgcac ggcagcgctc atcacaagga
     6901 gtggatgtgg cattcgcgct cagataacgc gatcctggtc aacggcgaaa gccagatcaa
     6961 gcactcggcg gacgcgcgcg gcgaactcat cgccttccac acctccccgg cactggacgt
     7021 catagcaggc gaggccagcg cctcctacga gcacctcgat ctctggaaac gccgcattat
     7081 cttcttcaaa ccccacgggt tcctcatcca cgatatcctg cgagcacccg aaccgagcca
     7141 gtttcaatgg acgctccaca ccaaaggcgc gatgaacatc gagggcaacc gggtttggtg
     7201 ggaaggcgca cccgggcggg ttgatgtcga tttcgcgtat ccggccggcc tcgtgattac
     7261 gcagaccgat cagtacgagc cgccgcccgc ggaatggacg ggctggaatc tcggcgaatg
     7321 gcatctcagc gcggagacgc cggaccgcgc atcgcagcag gaattcgtca cgtttgttgc
     7381 ggttcagggt gcggccgcgg ccattgacgc cgcccattcg gccctgccgc ggaacatcag
     7441 cgtggcgctg cccggcggta cggccacagt cgcgctgagt gatgcggcgt tcacggtgac
     7501 tcttcccggc gctgagccac agaccttcgc tgagagccgt tagacggccc ccgcgcagcg
     7561 aaaggaacgg cgactatttc cgcagcaggc ctcccagggc ctgttgcgcc tcgacgaccg
     7621 ccagcgcggt catgttgacg acatccgctt cgttgaagcc gccatactgc atgatatgga
     7681 ccggtttgtt catgccaagc aggatggggc cgatgacttc ggcgccgctg aacttctggg
     7741 ccagcttgaa cccggcatta ctcgagttca ggcacgggaa aatgaggatg ttcgccccgc
     7801 ccggcaaatt cgagaacgga aagctttccc ggagcagttc gggggaaatg gcgatatcgg
     7861 cctgcatctc gccgtcgatc agcaggcccg gagcgcgttc ccgcacccgt tccacggcac
     7921 ggcgcatttt ctcggcctcg ggcgcctgag cactgccgaa attactgaac gagaccatgg
     7981 cgatgcgcgg ttcgagatcg aagtcgcgca cggtgtctgc acagaggatc gcaatatcgg
     8041 ccaattcctc gtccgtgggt tcgatggtga cggagacgtc ggcaaaacag tacacatgat
     8101 cccggatggc gagcatggtc atgccggcga cccggctgac gtcgggacgt gcgccgataa
     8161 tctgcagggc agggcgcagg gtgtccgcat agctcgtggt caggccggct accatgcc
//
LOCUS       DAOPBQ010000091         8151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8110_length_8151_cov_4.269516, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000091 DAOPBQ010000000
VERSION     DAOPBQ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8110_length_8151_cov_4.269516"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PKW60_04440"
     CDS             complement(<1..602)
                     /locus_tag="PKW60_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98709.1"
                     /translation="MMTVLLLTTGCAFLLSAAASDRVATQDFAREIERALPPEQSYDY
                     HKRLSEAPVHVPVRNPEAVAAAGETAFPDEGWRLIWDRRSGTVLQTAIEDFQDYMKVS
                     MGVQVAVEGLDSLENWHDSARCIVVGTRDQLPGCGAALTGPKDYELTVTPERVTVCGF
                     DERGAMFGLYNLEARMNLREGPFLPADLSVVRHSLYDVRLV"
     gene            933..3092
                     /locus_tag="PKW60_04445"
     CDS             933..3092
                     /locus_tag="PKW60_04445"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPJ98710.1"
                     /translation="MRFGCSAAALLILACALAHSGENGYELPFDVGVFEQQVHRAYTT
                     QDGLPDNDVFRMARDTHGAILAQTARGTALFRAGLWEVEPQLSPEVFSKLELDPEQRA
                     RLREVAGADVDIRAVAEHRGELAVACDSGLYLSEGDEWHLALPCEGLVRWAPVDVRAV
                     AYDSTGQLWFAAPQGVGYRVSPTDWRLFTGADGLPYNDFTCMAAGPKGVWFGTSNGAI
                     QFRDGHWMFRQGRRWLLDNHVRDIAIDSDGNAWFATARGVSQIASESMTFAAKAAFFR
                     DEIEKYHRRTRLGYVNPAQLAAPGDKRSAVPVYTDNDGHFTGLYLGAVSLGYAATRDE
                     TLRRDAIEAFRALAFLSEVTSGGTHPAPRGFIARAVLPTSEPDPNPLFDRAYDLRRRE
                     RDALWKIIQPRWPIDASGEWYWKNDSSSDELDGHFFGFAIYFDRVCQAEAERDEVRQA
                     VRRIIDHLLDHGYNLVDHDGQPTRWGHFSPDDLNRNEAWCAERGLNSFSILTYLTIAH
                     HITGDPKYRKAYLELALEHGYGMNGMTQPGYLSGPGCVGHQPDDNMAFMNYYHLIRYE
                     TDPRLLNMYHFAIMTHWQHEKRERNPFTNFVYAACCLGKTRTSQWGTVDLTPQKHCFD
                     DAIDTLKRYPLDLVDWPMSNAHRIDISPILDETGQPTGRGGRCDGQVFPIDERHETYW
                     DLDPWALSYDGKGTRLREGVPYLLAYYMARAHGFLGD"
     gene            3315..5915
                     /locus_tag="PKW60_04450"
     CDS             3315..5915
                     /locus_tag="PKW60_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPJ98711.1"
                     /translation="MKMTELLRYDIPPEVIRLWQTRESENLLPIQELAIKRHNLFGDG
                     NLLIQAPTSSGKTFVGELAAIQTALRRKKVVYLVPLKALAEEKHLDFEQKYAPYGLQV
                     IISTRDHRQFDAQLESGDFSIAIVVYEKLSQVLVRRPERFTEVELVIADELELLSDPE
                     RGANVELLLTQVLRSGCRIIGMSAVLGEADKLAGWMHAELVQHDRRPVELRYGVLHEG
                     LFRYRTYNELSEGEERLTDSDLECSTWETLMENVATFTALEEPCLIFVKAKHESRRGA
                     ELLARRLSLPAAELTIERLAQLESTHSRDTLMETLASGIAFHNADLSPEERRAVEEGF
                     RQGEIKALVSTSTLATGMNLPAQNVFMTSEKWQYDSRFSLPWKTPILRGEYENMGGRA
                     GRYGSGNAFGRSILIAPTPFDRETYWRRYVEGDRECIQPQLAESALEDHALRLVASHA
                     CRSEEALRTFFESTLTGQWRWSTTLTLDEVEFRIRSAVNLAVDAGMLARVGDQELEAT
                     PFGLAVTAKGTTIATGLQLAAWIRESETRHWSAIDLLLAMALTPDARGPYLSLTTREY
                     DHADYPGRLKRLTLDEDLSADVPLNRLRNCDLMPFFEEVRAIKATLVMLEWMDQASLY
                     DIEEAFRVFSGQIFATADQLGWLIDATAAIATAFGAQEPFIKRLLTFAERVQWGVRDE
                     LLPIARLRLKGVDRNTLLALQAQRLDSPEALHAATEAQVARWAGTANARVLKTWAQRE
                     IQHAAPGSDASPPPPVLIVDERHPGQVTLDGQIVRLQDKQYRLLRILAETPGECVPYD
                     RIYEAVWGDTVVEPNQMHFQKRKILDRVKNVLPARTKLIRTVPKRGFVLELAPEEVML
                     AAGAPSPTAA"
     gene            6506..>8151
                     /locus_tag="PKW60_04455"
     CDS             6506..>8151
                     /locus_tag="PKW60_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPJ98712.1"
                     /translation="MRRRLSRRHFLTAGALTAASGALSCGQQKSGPAEAPARTEPETP
                     APSAAQSPRENGRVIILGFDGVEPTIVESMMDAGRLPHLTQLRDNGAFTRLRSTIPPQ
                     SPAAWNSFATCKNPGGHGIYDFIRRNPATHLPEIGTSKILHPSKRHDGTVTERAKAVG
                     FRKGDPFWVVADRAGVRCKLFNVPYAFPADHLEHGKMLCGLDVPEIRGSQNSFSAFSD
                     AYTPEQLQEDVPGGTRYLLEFVDNKATVDVRGARIPKGGQGARRERSEDTTLPLKFTV
                     NRVARTLTISSQDNTVTLTEGAWSEWLEWTFEPAREYIVRAISRFYLMEAGERVRLYM
                     SAFQFHPHAPYAPMSCPDEFTSELAERYGLFKTLGWAHETHALREGELTEAAFMEDAL
                     RTMDWRAMLTLDELDRDDFDILISAWTATDRIAHMFWRYRDPEHPLYDAEEAELFGDA
                     LERSYEKMDEIVGKVIPKLRENDLFIVMSDHGFETFRKGFNINTWLVRNGYAAVQGQD
                     DPATAVGEKGFLLDFDWSRTRAYAIGLSSMAFREMTLLAA"
BASE COUNT         1652 a         2668 c         2367 g         1464 t
ORIGIN      
        1 accagccgca cgtcgtagag gctgtgccgc accacactca aatccgcggg cagaaacggt
       61 ccttcacgaa ggttcatccg ggcctccagg ttgtacagcc cgaacatggc cccccgctca
      121 tcaaatccgc acaccgtcac gcgttcggga gtcaccgtga gctcgtagtc tttgggaccc
      181 gtcagcgccg cgccgcatcc cggcagctgg tcgcgcgtcc ccacgacaat gcagcgcgcg
      241 gaatcgtgcc agttctccag tgagtccagg ccttcgacgg ccacctgaac gcccatggag
      301 accttcatat agtcctgaaa atcctcgatc gccgtctgca gcaccgtccc gctgcgccgg
      361 tcccagatca gccgccatcc ctcgtccggg aatgccgtct ctccggctgc ggcgacggct
      421 tccgggtttc gcaccggcac atgcaccggg gcttccgaca gccgtttgtg ataatcgtag
      481 ctctgttcag gcgggagagc gcgctcaatc tcccgggcga aatcctgcgt tgccacccgg
      541 tccgacgcgg ccgccgaaag caggaaggca cagccagtgg tcaaaagcaa cacggtcatc
      601 atcctcgcct ttctgaatcg aacccggcac agccaaccgc cgggaacccc gttgacccac
      661 gcccatccag ccccgactcg aatccatccc cgcccccgtg tatccgggca gccggcccag
      721 ccccacgcac gaaccaacgc catttgagca gaatccgcgc atcatcacaa gtccggagcg
      781 gccatttctc ttttgcgggg acaacagctc cggaggcgat ttttccgctg cacaaagtaa
      841 ggtatcatgc ggaccgcgct tctgcaggga gagaaacacc tgtgaacgcg cggtacacgg
      901 ctccgtgccc aaccgcgccg agggaggatc ccatgcgctt tggatgttcc gctgctgcac
      961 tgctcattct cgcctgtgcc ctggcccaca gcggggagaa cggatacgaa ttgccgttcg
     1021 acgttggcgt attcgagcag caggtccacc gggcctacac aacgcaggac gggttgccgg
     1081 ataacgacgt gttccggatg gcgcgggaca cccacggcgc aattctcgcc caaaccgcgc
     1141 ggggaacggc gctttttcgc gcggggctct gggaggtgga accgcagctt tccccggaag
     1201 ttttttcaaa gctcgaactc gaccccgaac agcgcgcgcg cctgcgggag gttgccggcg
     1261 ccgacgtgga cattcgcgcc gttgccgaac accggggcga gctcgccgtg gcctgcgact
     1321 ccgggttgta tctgagtgag ggcgatgagt ggcatctggc tcttccctgc gaaggactgg
     1381 tgcgctgggc gccggtcgac gtgcgcgcgg tcgcctatga ctccacggga cagctgtggt
     1441 tcgcagcgcc ccagggcgtc ggataccgcg tctcccccac ggactggagg ctgtttacgg
     1501 gcgcggatgg cttgccttac aacgatttca cctgcatggc cgcaggcccc aaaggcgtct
     1561 ggttcggtac cagcaacggg gcgatccagt tccgggacgg acactggatg ttccggcagg
     1621 ggcggcgctg gcttctcgat aaccacgtgc gtgacattgc catcgactcc gacggcaacg
     1681 cctggttcgc caccgcgcgg ggcgtgtcgc agatcgcatc tgaatccatg actttcgccg
     1741 ccaaagcggc ctttttcagg gacgaaattg agaaatacca ccgccgcacc cgtctcggtt
     1801 acgtaaaccc cgcccaactc gctgccccgg gtgacaaacg cagcgccgtg cctgtatata
     1861 ctgacaacga cgggcatttc accgggctgt atcttggagc ggtaagcctc gggtatgccg
     1921 ccacccgcga cgagacgctg cgccgcgatg ccatcgaggc gtttcgcgcg ctggcttttc
     1981 tcagcgaagt cacgagcggc ggaacccacc ccgcaccgcg cgggttcatc gcacgggccg
     2041 tgctgcccac gtcggagccg gaccccaacc ctctgttcga ccgggcctac gacctccgcc
     2101 gcagagagcg cgacgccctc tggaagatca ttcagccacg ctggcccatc gacgcgtcgg
     2161 gcgagtggta ctggaaaaat gattcgagtt ctgacgagct tgacggccat ttcttcgggt
     2221 ttgccatcta tttcgaccgc gtctgtcagg ccgaagcgga acgggatgag gtgcgccagg
     2281 cggtccgccg catcatcgac cacctcctgg accacggtta caatctcgtg gaccacgacg
     2341 gccagcccac ccgctggggc cacttttccc ccgatgatct gaacagaaac gaggcctggt
     2401 gcgcggaacg cggcctgaat tcgttcagca tcctgaccta cctcaccatc gcccaccaca
     2461 tcacaggcga ccccaagtac cggaaggcgt atcttgaact cgctcttgaa cacggctacg
     2521 gcatgaacgg gatgacccaa cccggatatt tgtccgggcc aggctgtgtg ggccatcagc
     2581 ccgacgacaa catggccttc atgaactact accacttgat tcggtacgag acggatccca
     2641 ggctgctcaa tatgtaccac ttcgccatca tgacccactg gcagcatgag aagcgcgaac
     2701 gcaacccgtt tacgaacttc gtctatgccg catgctgtct cggcaaaacc cggaccagcc
     2761 agtggggtac cgtggatcta acgccgcaaa agcattgctt cgacgatgcc atcgatacgc
     2821 tcaaacgcta ccccctggat ctcgtggact ggcccatgtc caacgcccac cgcatcgata
     2881 tctcgccgat cctcgatgaa accggacagc ctaccggcag gggcgggcgc tgtgacggcc
     2941 aggttttccc catcgacgaa cggcatgaaa cctattggga cctcgatccc tgggcgctct
     3001 cgtacgacgg taagggtacg cgtttgcgcg aaggcgtccc ctatttgctc gcctactaca
     3061 tggcccgggc acacggcttt cttggcgact agtccaacac cgaaaacgag caattgacca
     3121 ggccgggatg gcgccgcccg cccatgccga tctgctgtgc aaccagcatg gttccatcct
     3181 ccagcacagc gcgaaatttc ccgacaggag actattttag tctggtattc atgaaattgc
     3241 gccgaaggct tgacatctga tgatattttc agtattttaa acgcggtgca gaaatggcgg
     3301 tggaggatga tgggatgaaa atgacggaat tgctccggta cgacattccg ccggaggtga
     3361 ttcgcttgtg gcaaacacgg gaaagcgaaa accttctgcc catccaggag ctggccatca
     3421 agcgccataa cttgttcggc gatggcaatt tgctcatcca ggcccccacg agttccggca
     3481 agacttttgt gggcgaactc gcggcaattc aaacggccct gcgccgaaaa aaagtggtct
     3541 atctcgtacc gctcaaagcc ctggcggagg aaaaacacct cgattttgag cagaaatacg
     3601 ccccttacgg cctccaggtc attatctcga cacgagacca ccgccagttt gacgcgcagc
     3661 tggaatcggg agatttttcc atcgcgatcg tggtctacga aaagctcagc caggtcctgg
     3721 tgcggcggcc ggaacggttc acggaagttg agctggtcat cgcggacgaa ttggagctgc
     3781 tgtccgatcc cgagcgcggg gcgaatgtgg agcttctcct gacccaggtt ctacggagcg
     3841 ggtgccggat catcggcatg tcggcggttc ttggagaagc cgacaagctg gccggctgga
     3901 tgcacgcgga acttgtgcag catgaccgcc gcccggtgga gctgcggtac ggcgtgcttc
     3961 acgaaggtct gttccgctac cgcacctaca acgaactgtc ggaaggcgaa gaacggctga
     4021 cagattcgga tctcgagtgc tcgacctggg agactctgat ggaaaacgtc gcgacattca
     4081 cggcgctgga ggaaccctgc ctgatcttcg tcaaggcgaa acacgaatcg cgccggggcg
     4141 cggaactgct cgcccggcgc ctctcgctgc cggccgccga actcaccatc gaacgcctcg
     4201 cgcagctcga aagcacccac tcgcgcgata ccctgatgga aaccctggcg agcggcatcg
     4261 ccttccacaa cgccgacctc tcgccggagg agcgccgcgc cgtcgaggaa ggattccgcc
     4321 agggcgagat caaagcactg gtatcgacca gcacgctggc gacaggcatg aacctcccgg
     4381 cacaaaacgt cttcatgacc tcagaaaaat ggcaatacga cagtcggttc agcctgccct
     4441 ggaaaacacc catcctccgg ggcgaatacg aaaacatggg cggccgcgcg ggacgttacg
     4501 gctcgggcaa cgcgttcggg cgctctatcc tcatcgcccc aacgccgttt gaccgcgaga
     4561 cctactggcg gcgttacgtc gagggcgatc gggaatgtat ccagccgcaa ctggcggaga
     4621 gcgcgctcga agatcacgcg ttgcgcctgg tcgcgtccca tgcctgccgc agcgaagaag
     4681 cgttgcggac cttttttgag agtaccctga cggggcaatg gcgatggagc acaaccctga
     4741 ccctcgacga ggtggagttc cggatccgct ccgccgtgaa cctggctgtc gatgcgggta
     4801 tgctggcccg cgtgggagac caggagctcg aagccacgcc cttcgggctc gctgtaacgg
     4861 caaaagggac caccatcgcc actggactgc aactggccgc ctggattcgg gaatcggaaa
     4921 cccgccactg gagcgcgatc gatctcctgc tcgcaatggc gctcaccccc gacgcccgcg
     4981 gcccctatct ctccctcacc acccgggaat acgaccacgc ggactatccc ggccgcctca
     5041 aacgtctcac cctggacgaa gatctctcgg cggacgtgcc gctcaaccgc ctgcgcaatt
     5101 gcgacctcat gccgtttttc gaggaagtcc gggctatcaa ggcgacgctc gtcatgctgg
     5161 agtggatgga ccaggccagc ctctacgaca tcgaagaggc gttccgcgtg ttctcagggc
     5221 agattttcgc cacggcggat cagctcggct ggctcatcga cgccacggcg gcgatcgcca
     5281 cggcgttcgg cgcgcaggag ccgttcatca aacgcctgct caccttcgca gagcgcgtgc
     5341 aatggggcgt ccgcgacgag ctgctgccca tcgcccggct ccggttgaaa ggcgtcgatc
     5401 gcaacacctt gctggcattg caggcgcagc gcctcgattc cccagaggcg ctccacgccg
     5461 cgaccgaagc ccaggtggcg cgctgggccg gcactgcaaa cgcccgcgtc ctcaaaacct
     5521 gggcgcaacg ggaaattcag cacgcggcac ccggctctga tgccagcccg cctccgcccg
     5581 tgctcattgt tgatgagcgc caccccggac aggtcacgct cgacgggcag atcgtccggc
     5641 tccaggacaa acaataccgg ctccttcgca tcttggcgga aaccccgggg gaatgtgtcc
     5701 cctatgaccg gatttacgag gcggtctggg gagatactgt cgtcgaaccc aaccagatgc
     5761 actttcagaa gcgcaaaatc ctcgatcggg tcaaaaacgt gctccccgca cgcacgaaac
     5821 tgatccgcac cgttccaaaa cgggggtttg tgctcgaact ggcgcccgaa gaggtgatgc
     5881 tggctgcagg cgccccctct ccgaccgctg cctgagcgat cggaggcgac ctggatcggt
     5941 catcccgctc taaatacaat aaaatcaatg gcgcccgagc cattgcccga tcaagcaaaa
     6001 agcccccgca atatgttaca attttggtgc agggataagg cggttcccag catcggcagt
     6061 acctcatttg aagcgcataa atggccagat cagggtaaaa tacccgatat gcggtgtgcg
     6121 aaactcatga aaatgcgctc cagcggtcta agcgcacacg cggcatcgag ttgccaaaag
     6181 caatgaaact tggcatcaca cttgcgtgtc taatgagatg aaagttgagg cttcataccc
     6241 acacgatccc aatcggccta ctgtggcgca tgaccaggtc tgaagttccc cactttggac
     6301 ccttccctcc cccgggaccg ggcgtcctcc ccagcgtccg gtcccggacg ctgtacagcg
     6361 acttgcgaag gcgccaccgc atcgatatag tctcctgagt attacctgcg cgcaacgcga
     6421 gagctggaat aagctctctc gggcctctgt tccgtctact caggataact aatgcataat
     6481 cagtcacttg caggggagcc acgcaatgcg aaggagactt tcccggcgcc atttcctcac
     6541 tgcgggagcc ctgaccgccg cgagcggggc gctctcgtgc ggccagcaga aatccggccc
     6601 ggccgaggcg ccggcccgca ccgaaccgga aaccccggct ccatctgccg cccagtctcc
     6661 ccgggagaac ggcagggtga tcattctcgg attcgacggt gtggaaccca cgatcgtcga
     6721 atcgatgatg gatgcgggcc gtctgcccca tctcacgcag ttgcgtgaca acggcgcatt
     6781 cacacgcctg cgctcaacca ttcccccgca gtctccggcg gcctggaact cattcgcgac
     6841 ctgcaaaaac cccggcggcc acgggatcta cgatttcatc cgcagaaatc cggccacgca
     6901 tctgcccgag atcggcacct ccaagatcct ccacccgtcg aagcgccacg atggcacggt
     6961 cactgagcgg gcgaaagccg tggggttccg caaaggcgat ccgttctggg tggtggccga
     7021 ccgcgccggt gtccgctgca agctgttcaa tgtgccgtat gcctttcccg cagaccacct
     7081 tgagcatggc aaaatgctgt gcgggctcga tgtgcccgaa atccggggct cgcagaactc
     7141 gttctcggcg ttttccgatg cgtacacccc tgaacaactc caggaggatg tccccggcgg
     7201 cacgcgatac ctccttgaat ttgtcgataa caaggccaca gtggacgtac gcggcgcccg
     7261 cattcccaaa ggcggccagg gcgcgcgccg cgaacgctcc gaagacacca ccctgccgct
     7321 caaattcacc gtgaaccgcg tcgcccgtac cctgaccatt tcgtctcaag acaacacggt
     7381 cactctgacg gagggggcgt ggtccgaatg gctggagtgg acattcgaac ccgctcgaga
     7441 atacatcgtg cgggccataa gccgcttcta tctcatggaa gcaggcgagc gggtgcggct
     7501 gtatatgtcg gccttccagt ttcatcccca cgccccctac gcgcccatga gctgtcccga
     7561 tgaattcacc agcgagctgg cggagcggta cggcctgttc aagaccctgg gctgggcgca
     7621 cgaaacccac gccctgcgcg aaggtgagct gaccgaagcg gctttcatgg aagacgccct
     7681 gcgcaccatg gactggcgcg ccatgctcac gctcgatgaa ctggaccgcg acgatttcga
     7741 tattctgatc tcagcatgga ccgcgaccga tcgcatcgcc cacatgttct ggcgctaccg
     7801 cgaccctgaa catccgctgt atgacgcgga agaggcggaa cttttcggag acgcccttga
     7861 acgatcctac gagaaaatgg atgaaatcgt ggggaaagtg attccgaagc ttcgcgaaaa
     7921 cgatttgttt attgtcatgt ccgaccacgg atttgagacg ttccgcaagg gatttaatat
     7981 caacacctgg ctcgtacgca acggttatgc cgccgtacag ggccaggacg accccgcgac
     8041 ggcagtcggc gaaaaaggct ttctgctgga tttcgactgg tcacgcacgc gtgcatacgc
     8101 catcgggctc agctcgatgg cgtttcggga gatgacgttg ttggcggcgg a
//
LOCUS       DAOPBQ010000092         8107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8167_length_8107_cov_3.091282, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000092 DAOPBQ010000000
VERSION     DAOPBQ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8167_length_8107_cov_3.091282"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1394)
                     /locus_tag="PKW60_04460"
     CDS             complement(15..1394)
                     /locus_tag="PKW60_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPJ98713.1"
                     /translation="MAATGAGLAACGMPRRARACPRDPRDRPNFVVIFVDDCGYRDVG
                     CFGAEGYETPHLDRMAAEGVRFTDFYAAAALCTPSRASLMTGCYPSRVGLPNVLNHTA
                     NIGLHEDETTIAEVLKSRGYATACYGKWHLGHHPKFLPTRHGFDEYFGLPYSNDMWPR
                     HPTYDAFPSLPLIEGEETIQYNPDQRQLTTWYTRRAVDFITAHKDEPFLVYLAHTMAH
                     VPLHVSDKFAGRAKSGPYGDVLMELDWSVGQILSTVRALGLDNNTLVVFISDNGPWLS
                     YGDHAGSALPLREGKNTTFDGGQRVPFIAWWPGQIAADSVCREVAVTFDMLPTLAGLA
                     GATLDPERTIDGKDIWPLLHGAPGAESPHEAFFYHLGGQVHAVRNGKWKLHAPHKYSS
                     LAKAGGGGWPGPYETKEIGGALFDLEADISETTDVADQHPDVVERLKGLMVAFEKDLA
                     DNSRPPGRI"
     gene            complement(1507..2892)
                     /locus_tag="PKW60_04465"
     CDS             complement(1507..2892)
                     /locus_tag="PKW60_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPJ98714.1"
                     /translation="MTHRVNRRSFIVAAGASMAAAAAAGNVAAQPPGPGLRNVLLYVV
                     DDQGRGDAGCYGNPVIKTPGLDRLAATGMLFTHGFCTTASCSPSRSVILSGLHNHANG
                     MYGLEHSCHHFTSLSNVESLPVRLSKAGYRTARMGKYHVGPEAVYHFDTNIKAPNIAP
                     ADMADACRDFIAEESGKPFFLYFCTVQPHRPFPREGADPVAPEEVVVPPFLPDTPECR
                     EELAQYYGSVQTADAGLLRLLDILEDTGRSKDTLVIYVSDNGIAFPGAKTTLYEPGIN
                     IPCVVRDPFAGTTGISTDAMITWADIAPTILDFAGVDAGEKQMHGRSFLPVLRGEPAS
                     GWDEIYASHTFHEVTMYYPMRAVRERRYKLIWNIAHGLAYPFASDLYQSATWQAVLRR
                     GDAYYGKRKVSAYVHRPAFELYDLETDPDEVNNLAGDPAHAETLERLKRKLRAFQERT
                     DDPWILKWERE"
     gene            complement(2938..3756)
                     /locus_tag="PKW60_04470"
     CDS             complement(2938..3756)
                     /locus_tag="PKW60_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPJ98715.1"
                     /translation="MTGREFGEKLRRGERVYGTAIVSPSPHWPSVVQQAGLDFVFLDT
                     EHITQDRVALSWMCRTYRALGIVPVVRIPRADPFLACQMYDGGACGLVAAYIETPEEV
                     RALVGATKYRPLKGKRLERVLQDPASLEPEMEAYVRERCADNILIVNIESRPAMDNLD
                     AILAVPGLDGVLVGPHDMTCNFGVPEQYHHPAFEEAMSELIAKARAANVGVGAHFFWD
                     TIEQEVAWAKAGANLLLHSGDISLFSAALNKDIGHLKKAMGDSVVKAGGEEVII"
     gene            complement(3872..4180)
                     /locus_tag="PKW60_04475"
     CDS             complement(3872..4180)
                     /locus_tag="PKW60_04475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HPJ98716.1"
                     /translation="MIHVIATIDIQPGKRAEFLELFKANVPNVLAEEGCVSYEPTVDV
                     EADTGAQPPLRDNVVVVVEQWANLACLHAHLHKPHMATYREQVKDLVRHVELRVLRSA
                     "
     gene            4344..5297
                     /locus_tag="PKW60_04480"
     CDS             4344..5297
                     /locus_tag="PKW60_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPJ98717.1"
                     /translation="MPDTDPPELRGNIFEIQRFSIHDGPGIRTTVFMKGCPMRCVWCH
                     NPESITPGPSLAFDPRLCIGCGYCFRVCPQGAHRMEGTAHILDRTRCAVCGTCTEECY
                     SQALELVGREMSVDDVMNVVVRDMPFYETSGGGMTLSGGEPAFQPDFSMGLLRAARDH
                     GIHTVVQTCGYAEARVFEAMMPLVDLFLFDIKETDPGRHIAFTGVSFRRIHENLRALH
                     DAGAAVLLRSPIIPGCNEREEFLAGIQALAASLPNLAGVELMPCNPLSESKVQRFGVD
                     PASRASTAPSNAGLVEHWCARLRAAGLRVLNEAPAIPNPLD"
     assembly_gap    5526..5535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5536..6282
                     /locus_tag="PKW60_04485"
     CDS             <5536..6282
                     /locus_tag="PKW60_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98718.1"
                     /translation="RTREENERRFTEQLSSLVCLTSELSTAPTVDDICCRAVEWGRAT
                     LSFDRIGIWLRADDPELIRGTWGVDENGLLQDERHLQAHISPESPEGRILLAKEAFVL
                     VERATFPSSDGTSEVTAPQLFAALWDGEKVIGHVSADNLLRDVAMTRNQCELLRMFGT
                     IVGYLCSKKRSELEREELIAELKAALKRIKTLQGLIPICAECRRIRDDEGSWTGLETY
                     VREHSEAEFSHGLCPECLKKVYAAERGEKA"
     gene            complement(6360..7316)
                     /locus_tag="PKW60_04490"
     CDS             complement(6360..7316)
                     /locus_tag="PKW60_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPJ98719.1"
                     /translation="MNEEPPCFSDEIVGVALAAGKGSRMMGLQTQLPKPVLPVLDKPI
                     VYHQLEAMVRLGIRKAIIVVGHRGYEVVRQIERQPPLGIEIDYVPQESNLGIAHCVGC
                     LEGAITGPFLLFLGDIFFHAPELGGMITVFREHQADAVLGAIEEQDVAAISRNYCIVT
                     DERGLARQVIEKPRYPKSRLKGVGIYLFTPVVFDAIRRTSRTAMRDEYEITDSIQILI
                     DLGYDVRPCTDIVHDLNVTYPRDLLDLNLWALEHEGLDKYVSDTARVGRGAAIEHAVV
                     GAGATVGAGAVLKNTVVFAGADVPPDARLEEVIVTECGVCRP"
     gene            7531..>8107
                     /locus_tag="PKW60_04495"
     CDS             7531..>8107
                     /locus_tag="PKW60_04495"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPJ98720.1"
                     /translation="MTRGACVNPKTQWLDVSMPLFEGMVCWPGDPEFETRAVSSLSKG
                     DGSNVTWLGLTTHTGTHVDAPRHIVPDGARLDELDPSVFFGPARVLDLTDAGPRIHPS
                     DFGEAPLPARLLLKTANGMLADRREFEPAFTALTTGAAARLVDEQVQLVGIDYISIAP
                     EGEDCEAVHRILLEAGVLIVEGLDLRGVQAGP"
BASE COUNT         1522 a         2506 c         2501 g         1568 t
ORIGIN      
        1 cgtgtgacgt tccgtcagat gcgtcccggg ggccgcgagt tgtccgccag atccttttcg
       61 aacgccacca tcagtccttt gaggcgttct acgacatccg ggtgctggtc cgcgacgtcg
      121 gtggtttcac tgatatcggc ttcgaggtcg aagagggccc ctccgatctc tttggtctca
      181 taaggcccgg gccagccgcc gccgcccgcc ttcgcaaggc tgctgtactt gtgcggcgca
      241 tggagtttcc acttgccgtt gcgcacggca tgcacctgcc cgccgagatg gtaaaagaac
      301 gcttcgtgcg gcgattcagc gccaggcgct ccatgcagca ggggccagat gtccttgccg
      361 tcgatagtgc gctccgggtc gagcgtggcg cccgccaggc cggccagcgt cggcagcata
      421 tcgaaggtaa ccgccacttc gcggcagacc gagtccgcgg cgatctggcc cggccaccag
      481 gcgatgaacg gcacgcgctg gccgccatcg aaggtggtgt ttttcccttc gcgcaacggc
      541 aaggcgctgc ccgcgtgatc tccataactc agccacgggc cattgtcgga aatgaacacg
      601 acgagggtgt tgttgtccag cccaagcgca cgcaccgtgg acaggatctg cccgaccgac
      661 cagtcaagtt ccataagcac atcgccatat ggccctgact tcgctcgacc ggcaaattta
      721 tccgacacat gcagcggcac atgagccatg gtatgggcga gatagaccag gaacggctcg
      781 tctttgtggg cggtgataaa atcgaccgcg cggcgggtgt accacgtcgt aagctgacgc
      841 tggtcggggt tgtattgaat cgtctcctcg ccttcgatga gcggcagcga cggaaacgcg
      901 tcgtacgtgg gatgccgcgg ccacatatcg ttcgagtacg gaagcccgaa atactcgtca
      961 aatccatgcc gggtcggcag gaatttcgga tggtggccga ggtgccactt gccgtagcag
     1021 gccgtagcat atccgcgcga tttcaggact tcggcgatgg tcgtctcgtc ttcgtgcagg
     1081 ccgatatttg ccgtgtgatt gagcacgttc gggagtccga cacgcgaagg ataacagccg
     1141 gtcatgagcg acgcccgcga aggggtgcac agcgccgctg ctgcgtaaaa atcggtgaac
     1201 cggaccccct cggccgccat gcggtcgaga tggggcgttt catagccttc tgctccgaaa
     1261 cagccgacat cgcggtagcc gcagtcatcg acaaagatga cgacgaaatt cggccggtca
     1321 cgcgggtctc ttggacaggc ccgggcgcgg cgtggcatgc cacaggcagc cagcccggct
     1381 cccgttgccg ccatgaactg cctgcgactc agtcttgaac ggaacatggg acattcccct
     1441 tttgaaaaag ggccgcggaa gcctatacag ctcccttccg gcccgcctct cagacaaatc
     1501 cgcttcttac tcgcgttccc atttcagaat ccatggatca tcggtgcgct cctgaaatgc
     1561 gcgcagctta cgctttaacc gttcgagggt ctccgcatga gcgggatcac cggcgaggtt
     1621 gttcacctcg tcgggatcgg tttccaggtc atacagctca aaggcggggc gatgcacgta
     1681 cgcggagacc ttgcgcttgc cgtaatacgc gtcgccgcgc ctcaggacgg cctgccaggt
     1741 cgcggactgg tacaaatccg atgcgaatgg gtaggccagc ccgtgcgcga tattccagat
     1801 caatttgtag cgccgctcgc gtacggcccg catggggtag tacatggtga cttcatgaaa
     1861 ggtgtgcgag gcgtaaatct catcccagcc ggaggccggt tcgccccgca gcaccggcag
     1921 aaacgagcgc ccgtgcattt gcttctcgcc ggcatccact cccgcgaagt ccaggatggt
     1981 gggggcaata tcagcccacg tgatcatggc atcggtcgag atgccggtcg ttcctgcaaa
     2041 aggatcccgc acgacgcacg ggatgtttat gcccggctcg tagagcgtcg ttttcgcccc
     2101 tggaaatgcg atcccgttgt ccgatacgta gatcacgagc gtgtccttcg agcggccggt
     2161 gtcctcgagg atgtcgagga gacgcagcaa tcccgcgtct gcggtctgca cgctgccgta
     2221 gtactgcgca agttcctcgc ggcactccgg cgtatcgggc agaaacggcg gcaccacgac
     2281 ttcttcgggc gccaccgggt ccgcgccttc gcgtggaaaa ggacgatggg gctggacggt
     2341 gcagaaatac aggaagaacg gcttgccgct ttcctccgcg atgaaatccc ggcacgcatc
     2401 cgccatatcg gcgggcgcga tgttcggggc tttgatgttc gtatcaaagt ggtacacagc
     2461 ttccggcccg acgtggtact tgcccattcg cgccgtgcgg taccctgcct tgctcaggcg
     2521 cacaggcagg ctttccacgt tcgaaagcga cgtaaaatgg tggcaggagt gttcaaggcc
     2581 gtacatgccg ttcgcgtggt tgtgaagccc ggagaggatc acagagcggc tcgggctgca
     2641 gctggcggtg gtacagaacc cgtgagtaaa cagcatcccc gttgcggcca ggcgatccag
     2701 gcccggcgtt ttgatcacgg ggttgccgta acacccggcg tcaccccggc cctggtcgtc
     2761 cacaacataa agcagcacat tgcgcaggcc tggccccggc ggctgggctg cgacgttgcc
     2821 cgccgccgcc gcggccgcca tgctcgcgcc tgccgcgacg atgaagcttc gccggttgac
     2881 tcgatgtgtc atggctatcc cccctggccc cggcacgaag tcctctgccc gggccggtta
     2941 gatgatgacc tcttctccgc ccgccttcac gacagagtct cccattgctt tcttgaggtg
     3001 gccgatatct ttattcaacg ctgcgctgaa caaggaaata tcaccgctgt gcagcagaag
     3061 atttgccccg gccttcgccc aggccacctc ctgctcgatg gtgtcccaga aaaagtgcgc
     3121 tccgacccca acgtttgcag cccgcgcctt ggcgatcaat tcgctcatgg cttcttcaaa
     3181 agcgggatgg tggtattgct ccggcacgcc gaagttgcag gtcatgtcgt gcggaccgac
     3241 cagcacgccg tcaagccccg gcacggcgag gattgcgtcg agattgtcca tcgccggccg
     3301 gctctcaata ttcacgatca ggatgttatc tgcgcaccgt tcgcgcacgt acgcttccat
     3361 ttccggctca agcgaggcgg ggtcctgcaa cacgcgctcg agccgcttgc ctttcagcgg
     3421 gcggtatttc gttgccccga ccagcgcgcg cacttcctcg ggcgtttcga tatacgcggc
     3481 cacgaggccg caggcgccgc catcatacat ctgacaggcc agaaagggat cagcccgtgg
     3541 aatgcgaacg accggcacga tgccgagcgc gcggtacgtg cggcacatcc acgacaaagc
     3601 cacccggtcc tgcgtgatgt gctcggtatc cagaaataca aagtccagtc ccgcctgctg
     3661 caccaccgag ggccagtggg gcgagggcga aacgatcgcc gtgccgtaga cccgctcgcc
     3721 acgccgaagt ttttcaccaa attctctgcc cgtcatgtgt tgttcctttt cgtttttctg
     3781 gagcggagcc gggatgctct ccaaacccag gggcgcgctg cggccccagg cggcctgtca
     3841 atgcgcatgg cgagggccgc gccggatttc atcatgccga gcgcagcacg cgcagctcga
     3901 catgccggac cagatctttg acctgctccc ggtaggttgc catgtgcggc ttgtgcagat
     3961 gggcatggag gcatgcgaga ttggcccact gttcgacgac cacgacgacg ttgtcgcgca
     4021 gcgggggctg cgcgccggta tcagcctcca cgtcaacggt gggttcgtag gagacgcacc
     4081 cctcctccgc cagcacgttg gggacgttgg ctttgaacag ctcgagaaac tccgcccgct
     4141 ttccgggctg aatgtcaatg gtcgcgatga cgtgaatcat gatacctcct gctgcgtagt
     4201 ctacttgggt ttgcacaaag aataccgaaa aagagcgcct gatgcacctc tctgctgaat
     4261 aaagttgatt tcagcacggc catgcgggaa agtaacggcg gccgcgacat gtatcgcggg
     4321 gaaaatgctc aggaaacgcc gatatgcccg atacagaccc gccggagctc cgggggaaca
     4381 tcttcgagat acagcgattc tccattcatg acggccccgg catccgcacc acagtcttca
     4441 tgaagggatg cccgatgcgg tgcgtctggt gccataatcc cgaatccatc accccgggac
     4501 ccagcctggc ctttgatccc cggctctgca tcggctgtgg ctactgtttt cgcgtctgcc
     4561 cgcaaggcgc tcaccgcatg gaagggacgg cgcacatcct ggaccgcacc cgatgtgcgg
     4621 tctgcggaac atgtacggag gagtgctact cccaggcgct ggaactcgtc gggcgcgaga
     4681 tgtccgtgga cgatgtgatg aacgttgtcg tgcgtgatat gccgttttat gagacgtccg
     4741 gcgggggaat gacgttatcg ggcggggagc cggcatttca gcctgatttc tccatgggtc
     4801 tgttgcgcgc tgcccgggac catggcattc ataccgtggt gcagacctgc ggctatgccg
     4861 aggcgcgcgt attcgaagcc atgatgccac tggtggattt attcctgttc gacatcaagg
     4921 aaactgatcc cggccggcat atcgcgttta ccggggtgtc gttcagacgc attcacgaaa
     4981 accttcgtgc gttgcatgat gccggcgccg ccgtgctcct ccgctcgccc atcattccgg
     5041 gctgcaacga gcgcgaggag tttctggcgg gcattcaggc cctggcggcc tcgctgccca
     5101 acctggccgg ggtcgagctc atgccctgca acccgcttag cgaaagcaag gtgcagcgtt
     5161 ttggcgtgga tcccgcttcc cgcgcctcca ccgcgccatc caacgccggc ctggttgagc
     5221 attggtgtgc gcgcctgcgc gctgccggcc tgcgggtcct caacgaggct ccagccatcc
     5281 caaacccgtt ggattgagcg cggttctcca agttctcggc acagcgagtg tctttatcgc
     5341 gatattcgca caattagtta ttcatgccgg gatctgccgc aatgtggtag aattctgtgg
     5401 gggattgcag cgcccaaaaa cggaggtgtg ttggaccatg gacgagaccg acattgcctc
     5461 gccggaaccg cccgagctgg aagaactgcg gcaacaggca cgcgacctgg taaaggcccg
     5521 gcgcgnnnnn nnnnncgcac acgcgaggaa aacgaacgcc ggttcacgga gcagttgtcg
     5581 tcgctcgtgt gtctcacgag cgaactgtcc acggcgccga cggtggacga tatctgttgc
     5641 cgcgccgtgg aatggggccg cgcgaccctc agctttgacc ggataggcat ttggttgcgg
     5701 gctgatgatc ccgagcttat ccgcggcacc tggggcgtcg atgagaacgg gctgcttcag
     5761 gacgaacgcc atctccaggc ccatatctcc cctgaaagcc cggaagggcg catccttctg
     5821 gccaaagagg ccttcgtgct ggtagagcgc gccacattcc cctcctccga cggcacatcg
     5881 gaagtcaccg cgccccaact gttcgccgcg ctgtgggatg gtgaaaaagt gatcggccac
     5941 gtgagcgctg acaacctgct gcgcgacgtg gccatgaccc gaaaccagtg cgaattgctg
     6001 cggatgttcg gcacgatcgt aggctatctg tgttcaaaga aacgttctga actcgagcgc
     6061 gaggaactga tcgccgaatt gaaggcggcc ctcaagcgca tcaagacgct ccaggggctc
     6121 atccccatct gcgcggagtg caggcgcatt cgcgacgacg aaggttcctg gaccgggctc
     6181 gagacctatg tgcgggagca ttccgaggcg gagttcagcc acggcctgtg ccctgaatgt
     6241 ctgaagaagg tctacgccgc agagcgcggc gaaaaagcgt aggccccgtt cgcgtccgtt
     6301 gccgcgcggc aggtctcaag cggcgcctgg atacctggtc cattcgcgca cacactcact
     6361 cacgggcggc agacgccgca ttcggtgaca atgacctctt caagccgggc gtccgggggc
     6421 acgtccgcgc ccgcgaaaac cacggtattc ttgagaaccg ccccggcgcc aacggtggct
     6481 ccggcgccca cgaccgcatg ttcgatggca gccccccgcc cgacgcgggc ggtatccgag
     6541 acgtatttat cgagcccttc gtgttccaaa gcccagagat tcagatcgag caggtcgcgg
     6601 gggtaggtca cgttcagatc gtgaacaata tcggtgcagg gacggacatc gtagccgaga
     6661 tcgatcaaga tctggatact gtccgtaatt tcgtactcat cgcgcatggc cgtgcgcgat
     6721 gtccggcgga tggcgtcgaa caccacggga gtgaacaggt agatgccaac acccttcagc
     6781 cggctcttgg gatagcgggg tttttcgatc acctggcggg ccaggccccg ctcatcggtt
     6841 acaatgcagt aattccggga gatggccgcg acatcctgct cttcaatggc gcccagcacc
     6901 gcatcggcct gatgttcgcg gaatacggtg atcatgccgc ccagttccgg ggcatgaaag
     6961 aagatgtcgc ccagaaacag cagaaacggc cccgtaatgg caccttccag gcatccgaca
     7021 caatgcgcga tgccgaggtt gctttcctgc ggcacataat cgatctcgat gcccagaggc
     7081 ggctggcgct cgatctggcg gacgacctcg tatccgcggt gccctaccac gatgatggcc
     7141 ttgcggatac ccagccgtac catggcctcc agctggtggt agacgatggg tttgtccagc
     7201 acggggagga ccggtttggg aagctgcgtc tgcaagccca tcatgcgtga gcctttgccg
     7261 gccgcaagcg caacaccgac tatctcatcc gaaaaacacg ggggctcttc attcacgggg
     7321 cctgtcctcc gcctgatgac cacgccgggg cgcggacact gttgaaccgg gaaccgccgg
     7381 gcagcatagc gcgctccgat cctgccatca actgcgcagc cccggcgccc tattcaggct
     7441 ccacgcacca ggtttacctt ttagcccgtg cggctggaat acggtataat ataacaggtt
     7501 gcagatatct gaacaccagg agaaggatgt ttgacaaggg gagcctgtgt caatccgaaa
     7561 acgcagtggc tggatgtctc catgcccttg tttgagggta tggtctgctg gcccggagac
     7621 ccggaatttg agactcgggc ggtatcgtcg ctttcgaaag gcgacgggag taatgtgacg
     7681 tggctcggcc tgaccaccca taccggaact catgtggacg caccccgtca catcgtgccg
     7741 gacggggcac ggctcgatga gctggatcct tcggtttttt tcgggccggc ccgcgtgctc
     7801 gacctgacgg atgcgggacc gcggatccat ccatcggatt tcggggaagc tcctctaccc
     7861 gcgcggttgt tgttgaagac agcaaacggt atgctggcgg acaggcggga gtttgagccg
     7921 gccttcacgg ctttgacgac cggcgccgcg gcacggctgg tcgacgaaca ggtacaattg
     7981 gtagggatag attatatttc cattgccccg gaaggggagg actgcgaagc ggttcatcgc
     8041 atccttcttg aagccggcgt actcatcgta gaaggcctgg atctgcgcgg cgtccaggcg
     8101 ggaccct
//
LOCUS       DAOPBQ010000093         8104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8171_length_8104_cov_3.967201, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000093 DAOPBQ010000000
VERSION     DAOPBQ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8171_length_8104_cov_3.967201"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKW60_04500"
     CDS             complement(<1..238)
                     /locus_tag="PKW60_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPJ98721.1"
                     /translation="MKRRGFTLIELLVVIAIIGILAAILLPALARAREAARRSSCQNN
                     LKQWGLVCKMFANESRGQVWPGRFKDIRKNYDPNG"
     gene            complement(543..1514)
                     /locus_tag="PKW60_04505"
     CDS             complement(543..1514)
                     /locus_tag="PKW60_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPJ98722.1"
                     /translation="MYKKKGFTLIELLVVIAIIGILAAILLPALARAREAARRASCAN
                     NLKQLGLMFKMYANEAKGEKWPTLSMYGENKNTPGLCDRPWSGHIFFEGMQVYPEYLT
                     DANVLICPSDSSGKEAIERKWRIGDDPSKELLPCKFNSTSYVYMGWAFNENMMNWPGG
                     DVNSGIFSNVTAFLDDEGSVDAGKVIPAMLGANLDPSGLAALYAVWNLVDTNLAYSHL
                     ADEDIDVQSVLTAAGMGAAFKPGSPSTVYRLREGIERFFVTDINNPSGSAQAQSDIWV
                     MWDITSVEVDQFNHIPGGSNVLYMDGHVEFVRFPDKAPVCRVLALIM"
     gene            1867..2934
                     /locus_tag="PKW60_04510"
     CDS             1867..2934
                     /locus_tag="PKW60_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPJ98723.1"
                     /translation="MTRGKHPTIYDIARQAGVSAKTVSKVLNDKDGVADETRARILAI
                     MDEVGYHPHIGARSLRGGGTACIGVTLPVSLEQVPISEEVLLWIFARLLRLFGSRGEY
                     ICFDINPSGHAPDRDYARGLWQQLYRGCVVAGPLPLNDTTARRIHDWGAPYLVLSRLD
                     GFPELSCATVDFELGAYESTRFLIKRGHKRIAMLKAFSGFQPGAERRRGYLRALEEAG
                     IEPDESLIRAVAFDAQDMTRCVHRLLLDREVTALIDCSVTEDGAALRSGARRAGRTPG
                     KDIEVLTWSYCEDEAVLPEACAQLWLPQLDAVGEGFDELAAWFHGDSEGPIRIVYRPT
                     LLEKVPDNEIASPRRLFTSQP"
     gene            3054..3974
                     /locus_tag="PKW60_04515"
     CDS             3054..3974
                     /locus_tag="PKW60_04515"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPJ98724.1"
                     /translation="MRIDVAAQTDIGRRKRKNEDFYGVFRDDSPGLQLFREGALLCVA
                     DGLGGHVAGEIASKLAVSILKDILKEPAPVSAEGLDSPGYLSLIKQYIVRANENVHKT
                     NQDLVKSGRPMGTTLLAALVTPRKAYIANVGDSRCYLFRGEELVTRTEDHSWVDEQVK
                     AGLMSKAEAESDSRKYILTRSVGTHPQVDIDDYVWDIQPGDMLLLCTDGLVNMVKDAE
                     ITEEFKRNASPAEIAHRLVNIANDNGGKDNITVIVADIMPSLGTVVARRLKAFSKKRG
                     AKLLYTLLFLLFGGLCLLAGYWLGQASVLR"
     gene            complement(3903..4703)
                     /locus_tag="PKW60_04520"
     CDS             complement(3903..4703)
                     /locus_tag="PKW60_04520"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPJ98725.1"
                     /translation="MKTIYLSDAHLSVSPDGRETMDAFVGFLRQLEPQDVARVVVLGD
                     LFDFWFEYRHAVFSGYFDVLRAFADLREAGVDLHFVCGNHDFWAGPFLEERIGFTVHR
                     DPVTLDIDGRRVHLAHGDGINPEDWRYHAYKRFARWGGAVRLFRLLHPDWAMAFAQRV
                     SRGSRRLFSPSGPGKSTEIGPLRAYAERLLAAGEAEAVVCAHSHHPERLEFATPDGTG
                     LYLNTGDWMWHRSYAVSEGGDFRLESAQNACLTEPIAGQETQAAEQQE"
     gene            complement(4700..5371)
                     /gene="lepB"
                     /locus_tag="PKW60_04525"
     CDS             complement(4700..5371)
                     /gene="lepB"
                     /locus_tag="PKW60_04525"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPJ98726.1"
                     /translation="MNSTEDNVFASQGAQPEPGPEPRERTRQEAKREMVEFVKMVVWF
                     LLLFLVLRGFVIEGYEVQGPSMEPTLYEQERILVLKLGQHWPFSGFLGPRPGDIVVFD
                     SPDDRGKRYVKRVIAMGPEVRSGNMVRADRNGGPEPGVRLRFRDTSIYVNDRKIDQSY
                     LAEDALSMEEDHEVLIGPDEYFVMGDNRNISKDSRNFGTVPENLLIGRALLRFWPLDR
                     FGFVK"
     gene            complement(5465..6577)
                     /locus_tag="PKW60_04530"
     CDS             complement(5465..6577)
                     /locus_tag="PKW60_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98727.1"
                     /translation="MLGRKRQAAAADKQVDMELAAKAVAKAACDGDIVNLRFLFGPFS
                     PARTASSERFETQKYSYLLPDDEQEEERSFQDALELVRSRGVWAHIENEWRMSRPPQL
                     PFELVLRLGDHAVRQGKYTSAAQAYEMLRIRARMQALFLEAADESLDHGDIARAVRGY
                     VVASGLEYDYAAFPEPLPKTPDYQMRALVLHGDYPERPEDCLALQETGQFVMTALSYL
                     LMSDETAARLEKRPESVRLAVLKELIECGDPAWDAFARRYREASELKREFAQRIQRAI
                     EARTAAQGASHKNLAGEIDDTLGADPGRIPIALLGREIEDGEWWQYLKELAFLHPAGI
                     LFVSRQFIGDREIIVPLYREDSPVARTLGLLPEAAS"
     gene            complement(6583..7167)
                     /locus_tag="PKW60_04535"
     CDS             complement(6583..7167)
                     /locus_tag="PKW60_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPJ98728.1"
                     /translation="MAGAGDGYINFYEILGLDEDAKPGEVRKVYRRRMKELVGEIARV
                     EITQQRRARYLLEMAQLNAALLVLRESDTREAYWSERQALIDLEQKWRDAVAEASDGA
                     DALRRQFDGRLQTFLSKYCEEWMLAAGQDKECVENSHWDAAHTRHASRILRFYRNDLY
                     QKILERLPYAEVTPPAVDWEERARFVRSALDERN"
     gene            7455..>8104
                     /locus_tag="PKW60_04540"
     CDS             7455..>8104
                     /locus_tag="PKW60_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98729.1"
                     /translation="MAKTKTTRGLDRRGFMKTAAAGTLILGARSALGAAANETLEIGI
                     IGCGGRGPWLGDLFQKHSATRVVAVHDPFEDRAAHAGDMLGVPENRRFTGLDGYHDLI
                     ASGVDAVAIISPPYFHPEQSVAALEAGKHVYLAKPVAVDVPGCNTIVGAADTHAATLC
                     TWVDFQTRQHPFYINAIQRLHDGLIGRPVMGQAYYYARRLNIKMEPGTEEARLRNWV"
BASE COUNT         1631 a         2466 c         2402 g         1605 t
ORIGIN      
        1 taccattcgg gtcgtagttc tttctgatgt ctttgaagcg gccgggccag acctgccctc
       61 ggctttcgtt cgcaaacatc ttgcacacca gaccccactg cttcaggttg ttttggcaac
      121 ttgacctgcg cgccgcttcc cgggctctgg cgagcgcggg aagaaggata gcagccaaaa
      181 taccaatgat ggcgatgacg acgagcaatt cgatcagcgt gaatcctctt ctcttcatag
      241 aatgctctcc ttacaggcga aactactgca gcttcttcct ctgggcttcc ggcaacacac
      301 cggccagagg tttcacgcta acagcaaagg caggatatgt caagacaata aaacagtaga
      361 tatggtagta tttcaggttt taataaaaaa gacggaattt ctgctttttg acaacaggag
      421 ctttcaaaaa taacaaaaaa tgagccgccg cagtgaagcg gcggctcaca tcgcctccgt
      481 ggcctgcttg ggggctgctg tggtgcgacc tggaggccgg cgatacgctg agtggacagc
      541 ccttacataa tcagggccaa aacgcggcag accggcgctt tatccgggaa gcgcacgaac
      601 tctacgtgtc cgtccatgta gagtacgttg cttccgccgg gaatatgatt gaactgatcg
      661 acttcgacgc tggtgatatc ccacatcacc cagatgtcgc tctgggcctg ggcactgccg
      721 gacgggttat tgatgtccgt gacgaagaag cgctcgatgc cttcccgcag ccgatagacc
      781 gtcgacgggc tccccggttt gaacgcggcg cccatgccgg cggcggtcag cacgctctgt
      841 acatcgatgt cctcgtcagc caaatgactg taggcgagat tcgtatcgac gaggttccac
      901 accgcataca aggccgcgag accgctcgga tccaggttag cgcccagcat ggccgggatg
      961 accttcccag catccacgct gccttcgtca tcaagaaacg cggtaacgtt tgagaagata
     1021 ccgctattta cgtcgccacc gggccagttc atcatatttt cgttgaacgc ccagcccatg
     1081 tagacgtagg acgtcgaatt gaatttgcag gggagcagtt ctttcgatgg atcgtcgccg
     1141 atgcgccatt ttctctcaat ggcttctttg ccgctcgaat ccgacgggca gatcagcacg
     1201 ttggcgtcgg tgaggtattc cggatagacc tgcattccct cgaagaaaat gtgcccgctc
     1261 cagggtctgt cgcataatcc cggggtgttt ttattttccc cgtacatcga gagggtgggc
     1321 catttctctc ctttggcttc gttggcatac atcttgaaca tgaggcccag ttgcttgagg
     1381 ttgttcgcac agctcgcgcg gcgcgcggct tcgcgggctc gggccagtgc cggaagcagg
     1441 atggcggcga gaatgccgat gatggcaata accacgagca attcgatgag tgtgaatcct
     1501 ttctttttgt acactactgc actccttctg aaaagcattg cgtcaatgcg ttgagaaacc
     1561 ccgcgtaacc ggcctgaaca gtaaccggtt accttctcga tggtatcaat catatttcga
     1621 attgtcaaga catttattaa taaaacctgt cctacaacag ctggctttgc tgctctgctg
     1681 gtattgccaa gtaacctatt accattcaaa aaccggccgg actccggtcc gaagccgtct
     1741 gaaactgagg cgtctgaagg ccggcagccg ggccacgttt tgaatgcgcg gtcgtcttca
     1801 ggtatgctgc ggctcggaga ggcatcaggg gaaacgggag ctgcgggaag aggattttca
     1861 ccagcagtga cacgcggaaa gcatccaacc atctacgata tcgccagaca ggcaggcgtt
     1921 tcagcgaaga ccgtgagcaa ggtgctgaac gacaaggacg gcgtggccga tgagacgcgc
     1981 gcgcgcattc tggcgatcat ggacgaagtc gggtatcacc cgcacatcgg ggcacggtcg
     2041 ctgcgcggtg ggggcaccgc ctgcattggg gtgacgctgc ccgtatcgct ggagcaggtg
     2101 cccatcagcg aagaggtgtt gctctggatt tttgcgcgcc tgttgcgttt gtttggctca
     2161 cgcggagaat acatctgttt tgacatcaat ccctcgggcc atgcacccga cagggattat
     2221 gcccgggggc tgtggcagca actgtaccgg ggctgcgttg tcgcggggcc tctgccgttg
     2281 aacgacacga ccgcccggcg cattcatgat tggggcgcgc cgtacctggt gctgagccgc
     2341 ctggatggct ttcccgagtt gagctgcgcc accgtggatt tcgagctggg cgcttacgaa
     2401 agcacccggt tcctgatcaa acggggccac aaacgcattg ccatgctcaa agcgttcagc
     2461 gggtttcaac cgggcgctga acgccggcgc ggctatctgc gcgctctgga agaagcgggc
     2521 attgagccgg atgaatctct cattcgcgcg gtggcgtttg atgcgcagga catgacgcgc
     2581 tgcgtccacc ggctcctgct ggatcgcgaa gtcaccgccc tcattgattg cagcgtgact
     2641 gaagacggcg ccgcgctgcg cagtggcgcc cgcagagccg gccggactcc cggcaaagat
     2701 atcgaggtgc tgacctggag ttattgcgaa gatgaggcgg tgctgccgga agcctgtgcc
     2761 cagttgtggc tgcctcagct cgatgcggtg ggcgaaggat tcgatgagct ggcggcgtgg
     2821 tttcacggcg acagcgaagg ccccatccgc atcgtgtacc gcccgacgct gctggagaag
     2881 gtcccggaca acgagatcgc gagccctcgc cgccttttta ccagccagcc ctgattccac
     2941 accgacgccc gcgacggcgg ggcgcacctg agcacagact gcgccgtcca gcccccggct
     3001 tgcgtaaaca gggcgaaatc tggtactttc cgtttgatca gggagcgaac cttatgcgaa
     3061 tcgatgttgc ggcgcagaca gatatcggac gccgtaaacg taaaaatgaa gatttctatg
     3121 gtgtgttccg cgatgactct ccgggattgc agttgttccg cgagggagcg ctgctgtgtg
     3181 tggcggacgg tctgggcggg catgtggcgg gtgaaatagc cagcaaactc gccgtatcca
     3241 ttctgaaaga cattctcaaa gagcccgcgc cggtgtccgc cgaaggactg gacagtccgg
     3301 gatacctgtc gctgatcaaa cagtatattg tgcgggccaa cgagaatgtg cacaaaacaa
     3361 atcaagacct cgttaaaagc ggccggccca tgggcaccac gctgctggca gccctggtca
     3421 cccccagaaa agcctacatc gccaatgtcg gcgattcgcg ctgttatctg ttccgcggcg
     3481 aggagctggt aacccgcacg gaagaccatt cctgggtgga cgaacaggtc aaggccgggt
     3541 tgatgtcgaa agccgaggcc gagtcggact cgcgcaagta tatcctgacc cggagcgtgg
     3601 gcactcatcc ccaggtggat atcgacgatt atgtctggga tatccagccc ggcgacatgc
     3661 tgctcctgtg caccgacggg ctcgtcaata tggtcaagga tgcggagatc acggaagagt
     3721 tcaagcggaa cgcgagcccg gccgaaatcg cccaccgtct ggtcaacatc gccaacgaca
     3781 acggcggcaa agacaacatc acggtcattg tggccgacat catgccgagc ctgggaacgg
     3841 ttgtggcgcg ccggctcaaa gcattctcaa agaagcgcgg cgcgaaactc ctctacacgc
     3901 tgctattcct gttgttcggc ggcctgtgtc tcctggccgg ctattggctc ggtcaggcaa
     3961 gcgttctgcg ctgattccag ccggaaatcc ccgccctcgc tgactgcgta ggaccgatgc
     4021 cacatccagt ccccggtatt caggtagagt cccgttccgt ccggtgtggc gaattccagg
     4081 cgttcgggat gatggctgtg cgcgcagacc acggcctcag cctctcccgc ggccagcagg
     4141 cgctcggcat acgcgcgcaa agggccgatc tccgtgctct tgcccggccc cgacgggctg
     4201 aagagccggc ggctcccccg cgacacacgt tgcgcgaagg ccatggccca atccggatgc
     4261 agcagccgga acaaacgcac cgcaccgccc cagcgggcga accgcttgta cgcgtgatac
     4321 cgccagtcct ccgggttgat gccatcgccg tgggccaggt gcacgcggcg gccgtcaatg
     4381 tccaaagtca ccgggtcgcg gtgtaccgta aatccaatcc gctcctcgag aaagggaccc
     4441 gcccagaaat cgtggttgcc gcatacgaaa tgaaggtcga ctcctgcctc acgcaggtcc
     4501 gcgaaggcgc gcaacacatc aaaatagccg gaaaatacgg cgtgcctgta ttcgaaccaa
     4561 aaatcaaaca ggtcgccgag caccacgacg cgcgccacgt cctgcggctc cagctggcgt
     4621 aaaaacccca cgaacgcatc catggtttcg cggccgtcgg gagacacgct gaggtgggcg
     4681 tccgagagat agatggtctt catttgacga aaccgaaacg atcgagcggc cagaaacgga
     4741 ggagtgcccg gccgatgagc agattttcgg gcacggtgcc gaaattgcgg ctgtctttgc
     4801 tgatgtttcg gttgtcgccc atcacgaaat attcgtcggg cccaatcagg acttcgtggt
     4861 cttcttccat ggaaagggca tcttcagcga ggtacgactg atcgatcttg cgatcgttga
     4921 cgtagatgga cgtgtctcga aaacgcagcc gcacgcctgg ctcgggtccg ccgttccggt
     4981 ccgcgcggac catgtttcca gaccgcactt cgggccccat ggcgatcaca cgcttgacat
     5041 accgcttgcc ccggtcgtcg gggctgtcaa agaccacgat atcgcctgga cgggggccga
     5101 gaaagccgga aaacggccag tgctggccca gcttaagcac gaggatgcgc tcctgctcgt
     5161 acagggtagg ctccatcgaa ggcccctgga cttcatagcc ctcgatgaca aacccgcgca
     5221 gcaccagaaa aaggagcaga aaccacacca ccattttgac gaactcgacc atttcgcgct
     5281 tggcttcctg gcgggttcgt tcgcgcggct caggcccggg ctcgggctgc gcgccctggc
     5341 ttgcgaacac gttgtcttcc gttgagttca ctgtgtttcc acttccatat ccaaaacgct
     5401 actcactgtt cgaaatctat catcccgccc tgttttctgc aaccccggca cgccggactg
     5461 gcactcagga cgccgcttcg ggcaacagcc ccagagtgcg cgctacgggc gaatcttcgc
     5521 ggtaaagcgg cacgatgatc tctctatctc caataaactg ccgcgatacg aagaggattc
     5581 cggccgggtg caggaaggcc agctctttca ggtattgcca ccattctcca tcctcgattt
     5641 cgcggccgag cagcgcgatg ggaatccgcc ccggatccgc cccgagggta tcatcgattt
     5701 cgcccgccag gtttttgtgc gacgcgccct gtgccgcagt acgggcctct atggcccgct
     5761 gaatgcgctg cgcgaattcg cgtttcaatt cggacgcttc acggtagcgg cgagcgaagg
     5821 cgtcccaggc cgggtccccg cattcgatca gctctttcag gaccgccaga cgcacgcttt
     5881 ccgggcgctt ttcgaggcgc gcggcagtct cgtcgctcat cagcaggtag ctcagggcgg
     5941 tcatgacgaa ctgacccgtc tcctgaagtg ccaggcagtc ttcgggacgc tccggatagt
     6001 ctccatggag caccagggcg cgcatctgat aatcgggcgt tttgggtaga ggttcgggaa
     6061 acgccgcgta gtcgtattcc agaccggacg ccacgacata accccgcacc gctctcgcga
     6121 tatccccgtg atcgaggctt tcatcggccg cttccaggaa cagtgcctgc atgcgggccc
     6181 ggatacgcag catctcatag gcctgcgcgg cgctcgtgta cttgccctgg cgtacggcgt
     6241 gatcgcccag cctcagaacc agctcaaacg gcaactgggg cgggcgcgac atgcgccatt
     6301 cgttttcgat atgcgcccag acgccccgcg aacgcaccaa ttcaagtgcg tcttgaaatg
     6361 accgctcttc ttcctgctcg tcatccggca gcaggtagct gtatttctga gtttcaaacc
     6421 gctcagacga cgcggtccgg gccggcgaga acgggccgaa cagaaaccgc agatttacaa
     6481 tatcgccgtc gcacgccgct ttcgcgacgg ccttggccgc gagttccatg tccacctgct
     6541 tgtcagcggc ggccgcttgg cgtttgcgtc ccagcacggt tgttaattcc tttcgtccag
     6601 cgcactgcgt acgaatcggg cccgttcctc ccagtcgacg gcaggcgggg tgacttccgc
     6661 gtagggcaga cgttccagga tcttctgata cagatcattc cggtagaaac gcaaaatacg
     6721 cgaggcgtgc cgcgtatggg cggcgtccca atggctgttc tccacgcact ccttgtcctg
     6781 gccggctgcg agcatccact cttcgcaata cttgctgagg aaagtctgga gccgtccgtc
     6841 aaactgcctg cgaagggcat cagccccgtc gctggcctcg gcgacggcgt cgcgccactt
     6901 ttgctcgagg tcgatcaggg cttgccgctc cgaccagtag gcttcacggg tatcgctctc
     6961 gcgcagaacc agcagggcag cgttaagctg cgccatttca agaagataac gcgcccggcg
     7021 ctgctgcgta atctcaaccc gcgcaatctc gccgaccaat tctttcatgc gacgccggta
     7081 gactttgcgc acttcacccg gcttggcatc ttcatcaagg ccaaggattt cgtaaaaatt
     7141 gatgtatccg tcccctgctc cggccatcgt cgcgcaactc ccgcgggttc attccacaaa
     7201 gtcccgattt tagctggaat gcccacacgg agacaaataa tcgccgggcg cagtgtctcg
     7261 ctgcgactgc tgcagcgggc agcacgatga gggtgaacga tcgcgcattc cgtgcgttca
     7321 ggcccgctct cgacagaggc ggtggtgcgt gcagggccgc acggctcacg aagcgtttat
     7381 ggcgcccgtg cagtccatgg tatgctgagc gctgatatct ggacaggctc aatgggcaaa
     7441 aggggacgcg catcatggcc aagacgaaga caaccagggg acttgatcgg cggggattca
     7501 tgaagaccgc cgcggcggga acactcattc tcggggcgcg ttcggcgctc ggagccgctg
     7561 cgaacgagac gctcgaaatc ggcatcatcg gatgcggcgg gcgaggtccg tggctcggcg
     7621 acctcttcca gaaacactcc gcgaccaggg tcgtcgcggt ccacgacccg ttcgaagacc
     7681 gtgctgcgca cgcaggggac atgctgggtg tgcccgaaaa ccgccgcttc accggactgg
     7741 atggctatca tgatctcatt gcgagcggag tcgacgccgt ggccatcatc agcccgccct
     7801 attttcaccc ggaacagagc gtagccgcgc tggaagccgg aaaacacgtc tacctggcca
     7861 aacccgtcgc ggtggatgtg ccgggctgca acacgatcgt tggtgcggcc gacacgcacg
     7921 cagccaccct gtgtacgtgg gtcgatttcc agacccggca acacccgttc tacatcaacg
     7981 cgatccagcg cctgcacgac gggctcatcg gccgccccgt catgggccag gcgtattatt
     8041 atgcgcgccg gctcaacatc aagatggagc ccggcaccga ggaagcccgt ctgcggaact
     8101 gggt
//
LOCUS       DAOPBQ010000094         8024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8249_length_8024_cov_4.226377, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000094 DAOPBQ010000000
VERSION     DAOPBQ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8249_length_8024_cov_4.226377"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKW60_04545"
     CDS             <1..451
                     /locus_tag="PKW60_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPJ98730.1"
                     /translation="TCNILMIGLGTGIAPFRAFVRHIYDERGGWKGKVRLFFGAKTGL
                     EMLYMNDERDDFANYYDEGTFKAFEAVSPRPAVDAPVALDKAIEQNAQEVWDMVRDPD
                     TYVFVSGHEKVGELLNQAMAKIAGSDEKWQRRKAEMKAGGRWMELMY"
     gene            complement(624..1220)
                     /gene="leuD"
                     /locus_tag="PKW60_04550"
     CDS             complement(624..1220)
                     /gene="leuD"
                     /locus_tag="PKW60_04550"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPJ98731.1"
                     /translation="MKPFTTLTGLVAPLEALNVDTDQIIPKQFLKRIARTGYEDALFY
                     DWRYLDDGKTLNPDFEMNAPRYQGASILLTKDNFGCGSSREHAPWALDNYGFRCLLAP
                     SFADIFYNNCFNNGLLPIRLPEAVIELLFQEVRAQEGYTLTVDLPAQTITRPNGETIA
                     FELDPFLKERLLNGWDQIALTLRHEEKIRAYEKSRRLV"
     gene            complement(1380..2792)
                     /gene="leuC"
                     /locus_tag="PKW60_04555"
     CDS             complement(1380..2792)
                     /gene="leuC"
                     /locus_tag="PKW60_04555"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013917705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPJ98732.1"
                     /translation="MARNIYDKIWDAHVVHEEPGQDTVLFIDRHFVHEVTSPQAFEGL
                     RLAGRAVRHPELTFATMDHNIPTTGRGKPIADLLSKTQVETLLGNCREFGITCFDMDS
                     EFNGIVHVIGPELGLTQPGFTIVCGDSHTATHGALGALAFGIGTSEVEHVLATQTLLQ
                     RKSRTMEVRVNGKLGPGITPKDVILAIIGKIGTAGGTGYVIEYTGDVIRDMSMEGRLT
                     VCNMSIEAGARAGLVSPDATTFDYLKGRRHLPKDKSHEELIREWSSWASDPGCSYDTV
                     VELDGARIEPQVTWGTSPGMVTGVNGAVPRLNEIEDANTRLAAKKALEYMDLPEGQPI
                     RDIHVNKIFLGSCTNGRIEDLREGAKVARGYKVNSSIEQALVVPGSRLVKVQAEKEGL
                     DTIFKEAGFEWREPGCSMCLAMNADVLNPGDRCAATSNRNFEGRQGKGGRTHLVSPAM
                     AVAAAIAGHFVDIRTWDYKE"
     gene            complement(3012..5846)
                     /locus_tag="PKW60_04560"
     CDS             complement(3012..5846)
                     /locus_tag="PKW60_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98733.1"
                     /translation="MKLRQRSLWWGMAGLLCVALGAYAQAAPGTGEAVVLENESFRYE
                     IAPDGRNVAFIDKASGTNYLKNDEPSHAASVTRASVVTECTRATFNDGLLILGFGDSG
                     VEAAVRVTMHARHALLEVEAVTGEGIDQLTFLNVPLSLEGTLEEPFACCALALNLQTH
                     VMEIPGPNNRLRAMCYPRFGLEGAEVAMIGCPQAHLRDVMKTVVSAAEAIPRSDIGGP
                     WALDGPINRGSYLFDFGQCTEETVGEWVALVKRLGLNQVDFHTGRSLRFGDYRPDPSL
                     FPDGRASVKAVIDALHEAGIAAGLHTYAFFIAKDTPYVTPVPDSRLGKDAAFTLADDL
                     TAGAAEVPVEESTEQMSTTTGFFVRNSVTLQIDDELIVYAGLSKEAPYAFTLCTRGAH
                     GTQAAPHGKGAKVHHLKECFGLFTPDADSTLLAEIAANTADTFNECGFDMIYLDALDG
                     EDILGGGENAWHYGSKFVFEIANRLNKPALFEMSTFHHHLWYVRARMGAWDHPNRSHK
                     KFIDIHAGVNLAGAKMFLPMNLGWWAVKTWTSPRNEPTFPDDIEYLLAKCIGTDAGLS
                     LMGVNPDNIDTTPAYQRLAPIFQRYEALRHAGAFSQAVKDQLKQPGMEFTLREDERGT
                     PVFQRVNYAKHTIRDTQGLTNRWTSTNPFGEQPAGLRIEALISAAPYDAPEAVVLEDF
                     SEPAETFANLRAQEDVEGALSRTTEHVKEGEASGLFTALSTRAVPVGSWVCCAKTYAP
                     DLDIAQQQGLGVWVHGDGQGELLNIQLKSPSHIAGGIGDHYVLVDFTGWRYFELIEHE
                     GSSIDDWVWPYGGAYAIYREDVDHAHIESLSLWYNNLPEGRSVTCYLSPIKALPLVDI
                     TLKNPSITVGGHTITFPAEIPTGSYLEFHGMADCKLYGANGEFLAEITPEGTALVLAA
                     GANPIEFTCEAPAVPNPRARVTVITRGDSI"
     gene            6148..7344
                     /locus_tag="PKW60_04565"
     CDS             6148..7344
                     /locus_tag="PKW60_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPJ98734.1"
                     /translation="MSHSLLICLPLALCAADLPETKSDMGNFYPMLTNYAAQSTFPLS
                     YLTQDWPDPEAWRQQGRARMHELLSYDPQPAPLNPELLETVKKDGYTRHLVRYAISAG
                     RTTEAYLLIPDGLEGPAPAIVALHCHSGFYYSGKEKITETENPLPCLQDLITNTYGGR
                     PFADELARRGFVVLVPDAFYFGVQRLDVTQLPPHFSEPLEGLEPGSNDYVRAFNPIAS
                     RHEHLLAKTMFAAGTTWPGILFQGDRASVDFLLTRPEVDSSRLGCVGLSIGGYRSAHL
                     FALDPRIKCAVVAGWMTTYSALLYDHLHSHTWMVYVPRQHNFLDLPDAVTLNAPNPLM
                     VINCLKDTLFTHEGMQAAETKIAAVYEKMGAADRFACKYYDEPHSFKVPAQEDAIAWL
                     QQWLVE"
     gene            complement(7381..>8024)
                     /locus_tag="PKW60_04570"
     CDS             complement(7381..>8024)
                     /locus_tag="PKW60_04570"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HPJ98735.1"
                     /translation="FHRFLRDERLADRDPSEGMDSPRLIRALPHVLSALEVERLLDAP
                     DLTRKQGLRDAAILELFYACGLRISELANLPLREVELEESMVRVRGKGSKMRIVPLGR
                     RAIERIQAYLPVRAEGKVLDDTLFLSTRGRRMSRTSVWQVVKRAAQAANIQQNVTPHM
                     LRHSFATHLLDHGADLRAVQEMLGHADVTTTQIYTHVSVDRLSRAHKDFHPRS"
BASE COUNT         1476 a         2635 c         2272 g         1641 t
ORIGIN      
        1 cacctgcaac atcctgatga tcggcttggg cacgggcatc gccccgttcc gcgcatttgt
       61 tcgccacatt tacgacgagc gcggcggctg gaaaggcaaa gtccggctgt tcttcggcgc
      121 caagaccggt ctcgaaatgc tctacatgaa tgatgaacgc gacgatttcg ccaattacta
      181 cgatgaaggg accttcaagg cgttcgaggc ggtgagtccc cggcctgccg tcgacgcgcc
      241 cgtggctctt gacaaggcca tagagcagaa tgcccaggaa gtgtgggaca tggttcgcga
      301 ccccgatacc tacgtcttcg tctccggaca tgagaaggtg ggcgaacttc tgaatcaggc
      361 catggccaag atcgccggtt ccgacgaaaa atggcaacgg cgcaaggccg agatgaaggc
      421 cggaggccgc tggatggagc ttatgtatta gatccgcagc cggtagaata cctactcacg
      481 gcgtctcgcc tccgcggcga ggcgccgttt tgtttgagct ggtcccattg aacgaagcgc
      541 acgcccgttt cggtaaaaat gtcacgtcgt cacgaagccc ggcatcacgg cgcccaccag
      601 acggattgcc cggaaagagg cgctcagacg agcctccggc tcttctcgta ggcgcggatc
      661 ttctcttcgt ggcgcagagt cagggcgatc tggtcccagc cattcaagag gcgttcctta
      721 agaaacggat cgagctcgaa tgcgatggtt tcgccgttgg gccgtgtaat cgtctgcgcg
      781 ggcagatcca cggtcagcgt gtagccttcc tgcgcccgga cctcctggaa cagcagctcg
      841 atgaccgctt ccggcagccg gatcggcagc aggccattat tgaagcagtt gttgtagaaa
      901 atgtccgcaa acgacggagc caggaggcac cggaacccgt aattgtcgag ggcccagggc
      961 gcgtgctcac gcgaggagcc gcagccaaag ttgtctttgg tcaacaggat gctcgccccc
     1021 tgataccggg gcgcgttcat ctcgaaatcg gggttcaggg tcttgccgtc gtcgaggtag
     1081 cgccaatcgt aaaacagggc atcttcgtac cctgtgcggg ctatccgttt gaggaattgc
     1141 ttcgggatga tctggtccgt gtcgacattg agcgcctcga gcggggcgac cagtcccgtc
     1201 aatgtggtaa atggcttcat gtcagttctc cttcctgaat tgccgctgcg ttcgcggcac
     1261 aggcttcgtt gcagcgcagc gggcggaccc gcaggccaaa ccagtgcccc gaagatgcgc
     1321 cgccgcactc gcagccgtgg ttcagatgcc tgccggcgat ccgcgccgcg aggccggtct
     1381 tactccttgt agtcccaggt gcggatgtcg acaaaatggc ccgcgatcgc tgccgcaacc
     1441 gccatggccg ggctgacgag atgtgtgcgg ccgcctttgc cctggcgtcc ctcgaagttg
     1501 cgattggagg tggctgcgca ccggtcgccc ggattcagca cgtcggcgtt catggccagg
     1561 cacatcgaac agcccggttc acgccactca aaccccgcct ccttgaaaat cgtgtcgagg
     1621 ccttccttct cggcctgcac tttgaccagc cgcgacccag gtaccaccag cgcctgttca
     1681 atgctcgaat tcaccttgta gccccgggca acctttgctc cttcgcgcag gtcctcgatc
     1741 cggccattcg tgcacgaacc caagaagatc ttgttgacgt ggatgtcgcg gatcggctgg
     1801 ccttcgggca ggtccatgta ttccagggcc ttcttcgccg ccagccgcgt gttggcgtct
     1861 tcgatctcgt tcaaccgggg cacggctccg ttgacgcccg tgaccatccc cggcgacgtg
     1921 ccccaggtca cctgcggttc gatgcgcgca ccgtcgagct ccaccaccgt gtcgtatgaa
     1981 cagcccggat cggaagccca cgatgaccat tcgcggatca gctcctcgtg gctcttgtct
     2041 ttcggcagat ggcgccggcc tttcaaatag tcaaacgtgg tcgcgtccgg cgacaccagg
     2101 cccgcgcggg ccccggcttc aatgctcatg ttgcacacgg tcagccggcc ttccatgctc
     2161 atgtcgcgaa tcacgtcgcc ggtgtattcg atcacatagc ccgtaccgcc cgccgtgccg
     2221 atcttgccga tgatcgcgag gatgacatcc ttgggggtga tgcccgggcc gagcttgccg
     2281 ttgacccgca cctccatcgt cctggattta cgctgcaaca gggtctgcgt cgccagtacg
     2341 tgctcgactt cgctggttcc gatgccgaac gccagcgcgc caagcgcccc atgcgtcgcc
     2401 gtgtgcgaat ccccgcatac gatggtgaat cccggctgcg tcaatcccag ttccgggcca
     2461 atcacatgca caattccgtt gaactcgcta tccatgtcga aacacgtgat cccgaattca
     2521 cggcaattgc caaggagggt ctccacctgg gttttcgaca gcagatctgc gatcggtttg
     2581 ccgcgtcccg tggtcgggat gttgtggtcc atggtggcga aagtgagttc cggatgccgc
     2641 actgcccggc ccgcgagccg cagcccttca aaggcctggg gcgaggtcac ttcatgaaca
     2701 aaatgccgat caatgaacaa gaccgtgtcc tgccccggct cttcatgcac cacatgcgca
     2761 tcccagattt tgtcgtaaat gtttcttgcc attcgtctta cctcaatgct gccagatatg
     2821 gttgcgtccc gggtcaactg ctccgcgtca gaccatcaag cgtgcgcctc aaccaggccc
     2881 tttacttaac gaacattatg cacacggcat agcttaccgg aattcgcacc cgttacgcca
     2941 cattacgctg cctcagaggg ttaagcgggc gagcccgaag gcgccgccgc gccatcccgg
     3001 aggaagtctt ctcagatgga atcgccccgc gtgatcaccg tcacccgggc ccgtggattc
     3061 ggcaccgcgg gcgcctcgca cgtgaactcg atcgggttcg caccggccgc gaggactaga
     3121 gccgtccctt cgggcgtgat ctccgcgaga aactcgccat tcgccccata cagcttgcaa
     3181 tcggccattc cgtggaactc gagataggaa cccgtcggga tctcagcggg aaatgtgatg
     3241 gtatggccgc ccacggtgat cgacgggttt ttcagcgtga tgtcgacgag tggcagggcc
     3301 ttgatcgggc tcagataaca cgtgaccgac cggccctccg gaagattgtt gtaccacagc
     3361 gacagggatt cgatgtgggc atgatccacg tcttcccgat agatggcata ggccccaccg
     3421 taaggccaga cccagtcgtc gatgctgctt ccttcatgct cgatcagctc aaaataccgc
     3481 cagccggtga agtccaccaa cacataatgg tctccaatgc cgcccgcgat gtgggacggg
     3541 cttttcagct ggatgttgag caattctccc tggccgtcgc cgtgaaccca caccccgagc
     3601 ccctgctgct gcgcgatgtc caggtcgggc gcataggttt tggcacagca aacccacgac
     3661 cccacgggga cagctcgtgt gctcagcgcc gtaaacaggc cgctggcctc accctctttc
     3721 acgtgttccg tggtgcggct gagcgctcct tccacgtcct cctgcgcccg caggttcgca
     3781 aacgtttctg cgggctcact gaaatcttcc aataccaccg cctccggggc atcgtagggc
     3841 gccgcagata tcaacgcctc gattcgcaga cctgccggct gctccccgaa cggattggtg
     3901 ctggtccacc ggttcgtaag cccctgggta tcacggatcg tgtgctttgc ataattcact
     3961 cgctgaaaca caggtgtccc gcgctcatcc tcgcgaagtg tgaattccat gccgggctgc
     4021 ttgagctgat ctttcaccgc ctgggaaaag gcgccggcat ggcgcagtgc ctcgtagcgc
     4081 tgaaagatcg gcgccagccg ctgatacgcg ggggtcgtgt cgatgttgtc cgggttcacc
     4141 cccatcaggc tcaggccggc gtcagttccg atgcatttcg cgaggagata ctcgatatca
     4201 tcgggaaagg tcggttcgtt ccgggggctg gtccaggtct tcacagccca ccagcccaga
     4261 ttcatcggca gaaacatttt cgcacccgcc agattcaccc cggcatggat gtcgatgaat
     4321 ttcttatgcg accggttcgg gtgatcccag gcgcccatcc gcgcccgcac ataccacaga
     4381 tgatgatgaa aggtgctcat ctcgaacagc gccggtttgt tgaggcggtt ggcgatctca
     4441 aacacgaatt tcgagccgta atgccacgcg ttctcgccgc ctcccagtat gtcttcaccg
     4501 tcgagggcgt caaggtagat catgtcgaac ccgcactcat tgaacgtatc tgccgtgttc
     4561 gccgcaatct ccgccagcaa cgtcgaatcg gcatcgggag taaacagccc gaaacattct
     4621 ttgagatggt gcactttggc gcccttgccg tgcggggccg cctgcgtgcc gtgtgccccg
     4681 cgcgtacaca gcgtaaacgc atacggcgct tcttttgaga ggcccgcgta tacaatcagc
     4741 tcgtcgtcga tctgcagtgt cacgctgttc cgcacaaaaa agccggtcgt ggttgacatc
     4801 tgctccgttg actcttcgac cggcacctcg gccgcgccgg ccgtcaggtc gtcggccagc
     4861 gtaaaggccg catccttccc caggcggctg tccggaaccg gcgtcacata cggcgtgtct
     4921 tttgcgataa agaacgcata ggtgtgcagc ccggccgcga tccctgcttc gtgcagcgca
     4981 tcgatgaccg ctttcacgct ggcgcggccg tcgggaaaca gagacgggtc gggccggtaa
     5041 tcgccaaagc gcagcgagcg gccggtgtgg aaatcgacct ggttcaatcc gagccgtttc
     5101 accagcgcca cccattcacc aaccgtctct tccgtgcact ggccgaaatc aaacaggtag
     5161 gacccccggt tgatcggccc atccagcgcc cacgggccgc caatgtcgga gcgcggtatg
     5221 gcctccgccg cgctcaccac ggtcttcatc acatcacgca aatgggcctg cgggcaccca
     5281 atcatcgcga cctccgctcc ttccaggccg aagcgggggt aacacatcgc gcgcaggcgg
     5341 ttgtttgggc ctggaatctc catcacatga gtctgaaggt tcagcgccag cgcgcaacac
     5401 gcgaacggct cctcgagtgt tccctcaagc gaaagcggca cgttcaaaaa cgtgagctga
     5461 tcaattccct cgccggtcac ggcttccact tccaggagcg catgccgcgc atgcatcgtc
     5521 acgcgcacgg cggcctccac gccgctgtcc ccgaagccca ggatcaagag gccgtcattg
     5581 aacgtggcac gggtgcattc cgtcacgacg ctggcgcgcg tcacgctcgc tgcgtggctc
     5641 ggctcatcgt ttttcagata attcgtcccc gatgctttat cgataaacgc cacatttcgg
     5701 ccatccggcg cgatttcgta ccgaaaactc tcattttcca gcaccaccgc ctctccggtc
     5761 cccggcgccg cctgcgcgta cgctccgagc gccacgcaaa ggagtccagc cattccccac
     5821 cacaacgacc gctgcctcag tttcatgtgc gttctccatt gcgctctcgc tgcattcgtt
     5881 tcttatccat ccccaccggc taaaccgcga gtacgataca ttccggcaag tccgcggccc
     5941 gtgcagccta ttttgccatg ccggggcgca gcatctcgac cacgacagtc gtgccggagc
     6001 gcgttcgtgc tgcttttggc caatcttcaa atctcgaaga ggcgtggatg ctctttttgc
     6061 acacatcttg cgcacggtct cagccaccac cactcatttt ttgatatagt ctgtatctat
     6121 tcctcacccc aacgctggag ggttgccatg agtcactcgc tgctgatttg cctgcccctg
     6181 gccctgtgcg ccgccgatct tcctgaaacc aagagcgaca tgggtaattt ttatcccatg
     6241 ttaaccaact atgccgcgca gtctaccttc ccgctgtcct atttgaccca ggactggccc
     6301 gatccggaag cctggcgcca gcaggggcgt gcccgcatgc atgaactgct ctcctatgac
     6361 ccgcagcctg cccccttgaa ccccgaactg ctcgagacag tgaagaaaga cggatatacg
     6421 cgccacctgg tccggtatgc catttcagcc gggcgcacga ccgaggccta tttgcttatc
     6481 cccgacgggc ttgaaggtcc cgcacccgcc atcgtcgctc tacattgcca cagcggtttt
     6541 tattacagcg gcaaagagaa aatcaccgag accgagaatc cgttgccctg cctccaggat
     6601 ttgatcacca atacctacgg tggccggccg tttgccgatg agcttgcccg ccgcggattt
     6661 gtcgtgcttg tgcccgacgc cttctatttt ggcgtgcagc gcctcgacgt cacgcaactg
     6721 cccccccact tctccgagcc tctggagggg cttgaacccg gaagcaatga ttacgtgcgc
     6781 gcttttaatc ctatcgcgag ccgccacgaa catctcctgg ccaagaccat gtttgccgcc
     6841 gggaccacct ggccgggtat cctgttccag ggcgaccgcg ccagcgtcga ttttctgctc
     6901 acccgtcctg aagtcgactc ctcgcggctc ggctgtgtcg gactttccat cggcggttac
     6961 cgcagcgccc atctgttcgc ccttgatccc cgcatcaagt gcgccgtggt ggccggctgg
     7021 atgacaacct acagcgcgct cctttacgac cacctccatt cccatacgtg gatggtctac
     7081 gtgccgcgcc agcacaattt cctggatctg cccgatgccg tgacgctcaa cgcccccaac
     7141 cccctcatgg tcatcaactg ccttaaagat acgctcttca cgcatgaagg gatgcaggcc
     7201 gctgagacca aaatcgctgc ggtctacgaa aaaatgggcg ccgccgaccg tttcgcctgc
     7261 aaatattacg atgaaccgca ctcgttcaag gtgcccgcgc aggaggatgc catcgcctgg
     7321 cttcagcaat ggcttgtgga ataggcgggc gggtccagtg actgttcttc ggtccgtgtt
     7381 tcaggaacgc ggatgaaaat ccttgtgggc ccggctcaag cggtccacgc tgacatgcgt
     7441 gtagatctgc gtcgtggtca cgtcagcatg gcccagcatt tcctgaaccg cgcgcaggtc
     7501 cgcgccgtga tccagcagat gcgtggcgaa ggagtgcctc agcatgtggg gcgtgacgtt
     7561 ctgctggatg ttcgcggcct gagcggcgcg cttgaccacc tgccacacgc tcgtccggct
     7621 catccgccgg ccgcgcgtgc tcaggaagag cgtgtcgtcg agcaccttgc cctccgcacg
     7681 caccggcagg taggcctgga ttcgttcgat cgcccgtctt cccagcggta cgatccgcat
     7741 cttggagccc ttgccccgca cccgtaccat cgactcctcc agctccactt cgcgcagcgg
     7801 gagattggcc agctctgaga tgcgcagccc gcaggcgtaa aacagctcca ggatcgccgc
     7861 gtcgcgaagg ccttgtttgc gcgtgagatc gggggcgtcc aacagccgtt ccacttccag
     7921 cgcggagagc acgtgcggca atgcccggat cagccggggc gaatccatgc cttcggaggg
     7981 atcccggtcc gccaggcgtt catctcgcag gaagcggtga aacc
//
LOCUS       DAOPBQ010000095         7984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8291_length_7984_cov_3.848152, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000095 DAOPBQ010000000
VERSION     DAOPBQ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8291_length_7984_cov_3.848152"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..891)
                     /locus_tag="PKW60_04575"
     CDS             complement(49..891)
                     /locus_tag="PKW60_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98736.1"
                     /translation="MSDRFNWLEFSAERENPEVQPPQAAPHDAPSFYQAARSMREAAH
                     LRAACDYYRKAAGLNDQHYEAWSELIDTLVRRGQLDEADRVSSEILQNYRQVRLFYAS
                     RALVLAHRHRTQEAFPLISVALEGDADAWYPRCVMGELLVRFDRGKRCEAQRFFDQAI
                     ERAKDPWEPYFLAGWAFLHTGLPALAAGHLAEAAHFRPRAALCWLYLGDSFRALRLYD
                     QAQFYYQCAQELEPDSELSIARQKDCGKLTFGLMRLFSRNKLRARWKTALEDHTREKE
                     WEEP"
     gene            complement(884..2422)
                     /locus_tag="PKW60_04580"
     CDS             complement(884..2422)
                     /locus_tag="PKW60_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPJ98737.1"
                     /translation="MHNGFSEELDRLIRARYTLLYVVTWEEDRALRILMRLAEAQRKP
                     LFEWSITNGLRCIHGAGSQGGGARTRDAMQMLNEVLQKDGPAIYVLKDFHSYLEAPEM
                     IRQIRDLGSALGPTRKTLVFLSPVLTLPRELEKDMTILDLPLPTYEELSQLLDDSIGR
                     PSSARKFQVQLKPQERDALVKAAQGLTLKEAENAFAQAIVRDNVLDAQDIQAVISEKR
                     QVIRKSGLLEYYGVDADLADVGGMDVLKDWLRKRVRAFSDEARAYGLPQPRGILLMGV
                     QGCGKSLVAKTIARQWQLPLLRMDMSMIFQGYIGSSEQNMRNATKTAESLAPVVLWID
                     EIEKAFSGVEGSGSSDAGTTARVVGTFLTWIQEKTAPVFVVATANEVRELPPELLRKG
                     RLDEIFFVDLPRSRERAEIFAIHLHKVRREPMKYDLPELVGASRGFSGAEIEQAIIAG
                     LHESFFDGRELETRDILKAIRDTVPLSRTMREKVEELRVWARDRARPVAKPPEKRPEK
                     DSDE"
     gene            complement(2422..3072)
                     /locus_tag="PKW60_04585"
     CDS             complement(2422..3072)
                     /locus_tag="PKW60_04585"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HPJ98738.1"
                     /translation="MYVGRIVSIAMTQDSRLCGVYRVSSRSFPNRTAVVRENRVSIVP
                     KPGHEADVHKNPYIAYNCVRVVCGGDVAVITNGSHTDPIAEKIISGMNPRDALLLSLM
                     TLDYEKDDYKTPRIAGVVDKRNGGSGYLGIVREDGLHVRRMPLEAGMFYYVATYEEGT
                     LSEAQSGMFPAKTAEEACQFLLGGGVFAERTHPVTAVAAVRSNSDFELAAMDAPHT"
     gene            3190..4482
                     /locus_tag="PKW60_04590"
     CDS             3190..4482
                     /locus_tag="PKW60_04590"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPJ98739.1"
                     /translation="MPITQREHARRLITQFKGDAYIYGVHCFEKLGEHARPLGSRAAV
                     VASGFGKPWGPPLHEAVRKSLAGAGITAGDIIPGARPNAPREDVFRIVEALEEQAPDL
                     VVAVGGGSTIDAAKAAVARLALAPDHPGLDEYFGSNQVTRLLADAGKRMLPVLAVQLA
                     SGSAAHLTKYSNITDEASAQKMLIVDDAIVPPRCLFDYAQTTTMSPEFTMDGALDGVS
                     HCLEVFYGAPGEALNTIRPVALLGIELLIRHVKQACAEPEDLEAREALGLGTDLGGYA
                     IMLGGTNGAHLTSFSLVDVLPHGRACALMNPYYTVFFAPAIEARLREVGEIYRAAGYT
                     RRNLAGLHGRDLGIAVAEAMLNLSRDIGFPVTLDEVPGFSPEHIERALSAARDPRLAM
                     KLQNMPVPLASDQVDDYMGPVLEAARTGDFELIRILTE"
     gene            4494..5663
                     /locus_tag="PKW60_04595"
     CDS             4494..5663
                     /locus_tag="PKW60_04595"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPJ98740.1"
                     /translation="MKIHFILGMVIIAALSATAWAASGAEGGASTVNVQHVKIYYEKG
                     RFGGWPANYGMWNWGNEILVGFSRGYYKDLGPTRHHIDREKPEEHWQARSLDGGETWS
                     LEYPAEQGRLIAQGTALHGIETPGLPIPEMRECPGGIDFSHPDFAMTLRMTDIDARSP
                     SKFFYSYDRGRTWEGPFILPNVGVNGIAARTDYVVDGKHECMLFQTAGKTDGTEGRPF
                     CMRTVDGGKTWEFLSWIMPEPEGFGIMPSTVRLSDDTLLTTIRRREISNRWIAAYISR
                     DNGRSWDFLNDPVDTLGEGNPPSLIKLSDGRLCLTYGVRMQPFRIAAKLSKDNGQTWS
                     DEIVLRDDGADRDIGYVRSLQRPDGKVISTYYIDEPTTGPERYIAATIWDPAEVE"
     gene            complement(5741..7192)
                     /gene="gnd"
                     /locus_tag="PKW60_04600"
     CDS             complement(5741..7192)
                     /gene="gnd"
                     /locus_tag="PKW60_04600"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HPJ98741.1"
                     /translation="MAQQDIGLIGLAVMGENLVLNMANKGFSVAVYNRTRDRVDNFIQ
                     GRAKGKSIVGCHSIEELVQNLQKPRRVMLLVKAGDPVDSLIEQLIPHLDPGDIIIDGG
                     NSHFPDTIRRTKYVESKGLLYIGTGVSGGEEGALNGPSMMPGGSLAAWPHVKEIFQTI
                     CAHTDEGEPCCDWVGENGAGHFVKMVHNGIEYGDMQMICETYQMMKEGLGMSNDEMHE
                     VFAEWNRGELNSYLIEITRDILAYRDEDGNAVLDLILDTAGQKGTGKWTAIAALDEGQ
                     PLTLIGEAVFARCLSALKDERVEASKQLRGETQPFSGDKKAFVDDLRKALYAAKIVSY
                     AQGYQLMRSAAKAYGWNLNYGGIALMWRGGCIIRSVFLGRIKEAFDKDPGLVNLLLDP
                     FFRDAVASAQSSWRNVVIEAAKMGIPVPAISTALAYFDGYRHERLPANLLQAQRDYFG
                     AHTYERIDRPRGEYFHTNWTGRGGDTAATTYTV"
     gene            complement(7398..>7984)
                     /locus_tag="PKW60_04605"
     CDS             complement(7398..>7984)
                     /locus_tag="PKW60_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98742.1"
                     /translation="LRRRGIPGWGKFHIKRESAGGPLIDIGVHVLDLIVWLMGCPKPV
                     SATGQVSRKFGDRKDLCNGEWGVPYPVEEFDVEDHAAAFIRFENGIALNLEVSWAANI
                     PKDVQGLRLLGDKAGVSTEPLIVTSAADGALTSLQYDWLPEQEGHRMEILHFAECVRD
                     GLPVRVRPQESLRIQQILDAIYESSRKGKEVAIK"
BASE COUNT         1508 a         2565 c         2285 g         1626 t
ORIGIN      
        1 tgtctttcgt ggccacccgt tcgaccaggg gcgacgcata cgtgtcggtc atggctcctc
       61 ccactccttt tcgcgcgtgt gatcttccag ggcagtcttc caacgtgccc gcaacttgtt
      121 tctgctgaac agccgcatca acccgaacgt gagcttgccg cagtctttct gacgcgcgat
      181 cgacaattca ctgtccggtt caagttcctg cgcgcactga taatagaact gggcctggtc
      241 atacagccgc agtgcgcgaa aactgtctcc cagatacaac cagcataacg ccgcacgagg
      301 gcggaaatgc gccgcctctg ccaggtggcc agcggccagc gcgggcagtc cagtatgcaa
      361 aaatgcccac cccgccagaa agtacggttc ccagggatcc tttgctcttt cgatggcctg
      421 atcgaaaaag cgctgggcct cacatcgctt cccgcggtcg aaccgcacca gcagttcccc
      481 cattacacag cggggatacc acgcatcagc gtcgccctcc agggccacgc tgattagcgg
      541 aaacgcctcc tgtgtccgat gccggtgcgc cagcaccagt gcccgggagg cataaaacag
      601 gcgcacctga cggtaatttt gcaaaatctc cgacgacacc cgatccgcct cgtcaagctg
      661 gccgcggcgc accagggtgt cgattaattc gctccacgcc tcataatgct ggtcgttcaa
      721 ccccgcggcc ttgcgatagt agtcgcacgc tgcccgcaaa tgagcggcct cgcgcatgct
      781 gcgcgcggcc tgatagaacg acggcgcgtc atgcggtgct gcctgagggg gctgtacttc
      841 cgggttctct ctctctgcgc tgaactccag ccagttgaac cggtcactca tcggagtcct
      901 tttccggccg cttttccggc ggcttcgcca caggacgcgc ccggtcgcgc gcccacacgc
      961 gcagctcctc gactttctcg cgcatcgtgc gggacaaggg aactgtatct cgaattgctt
     1021 tgagaatatc gcgggtctcg agttcacggc catcgaaaaa actctcgtgc aatcccgcga
     1081 taatcgcctg ctcgatctcc gcgccgctga atcctcgaga cgcccctaca agctcgggca
     1141 ggtcgtattt catgggttcc cggcgcactt tgtgcagatg aatggcgaaa atctccgccc
     1201 gttcgcggct gcggggcaaa tccacgaaga aaatttcgtc caggcggcct tttcgcagca
     1261 gttccggtgg caattcgcgc acttcgttgg ccgtggccac tacgaacacc ggcgcggtct
     1321 tttcctgaat ccaggtgaga aacgtgccga ctacacgggc ggtcgtgcct gcgtcggagc
     1381 tcccggagcc ttccacaccg gaaaaggcct tttcgatctc gtcaatccac aggaccaccg
     1441 gggcgaggct ttcggcggtc ttcgtcgcgt tacgcatgtt ctgctccgaa ctgccgatat
     1501 agccctggaa gatcatgctc atatccatgc ggagcaacgg aagctgccac tgccgcgcga
     1561 tggtcttggc taccaggctc ttcccgcagc cctggacgcc catcagcagg atgcctcgcg
     1621 gctgcgggag tccgtatgcg cgcgcctcgt cgctgaacgc acgcacgcgc ttccggagcc
     1681 agtctttcag cacatccatg cccccgacgt ccgccagatc cgcgtcgaca ccgtaatact
     1741 ccagcaggcc cgattttcga ataacctgcc gtttttccga tatcacggcc tgaatgtcct
     1801 gtgcatccag cacattgtcg cgcacgatag cctgcgcaaa cgcattctcg gcttccttca
     1861 gcgtaagccc ttgagccgct ttaaccaggg cgtcgcgctc ctgtggcttc agctgaacct
     1921 gaaacttgcg cgccgaactc gggcgcccaa tggaatcgtc caggagctgg ctcaattcct
     1981 cgtaggtggg taagggaaga tcgaggatgg tcatgtcttt ttccagctca cgtggaagcg
     2041 tcagcacggg ggacagaaaa acgagggttt tccgggtcgg cccgagcgcg ctgcccagat
     2101 cgcggatctg gcggatcatc tccggtgctt cgaggtagga gtgaaaatct ttcaggacat
     2161 agatggcagg gccatccttc tgcaacacct cgttcagcat ctgcatcgca tcgcgcgtac
     2221 gcgccccgcc gccctggctg cccgccccgt gaatgcagcg caggccattt gtgatggacc
     2281 attcaaacag cggttttcgc tgggcctcag ccaggcgcat gaggatgcgc agcgcgcggt
     2341 cttcttccca ggtcactacg taaagcagtg tgtagcgcgc ccggatcaac cgatccagtt
     2401 cttcgctgaa tccattgtgc atcacgtgtg gggtgcatcc atcgcggcca gctcgaaatc
     2461 cgaatttgag cgcacagccg ccacggccgt cacgggatga gtccgctccg caaaaacgcc
     2521 gccgcccagg agaaactggc aggcctcctc ggccgtcttt gcggggaaca ttccactctg
     2581 ggcctcggag agtgtccctt cctcgtaggt ggccacgtag taaaacatcc ctgcctccag
     2641 gggcatgcgc cgcacatgca ggccgtcttc gcgcacgatt ccgagatacc ctgagccgcc
     2701 gttgcgctta tcaaccacac ccgcaatgcg cggcgtcttg tagtcgtcct tttcataatc
     2761 cagtgtcatc agcgagagca gcagcgcgtc gcggggattc atgcccgata tgatcttttc
     2821 ggcgattggg tcggtgtggc tcccgttggt gattacggcc acatcgcctc cgcaaaccac
     2881 gcgcacgcag ttgtaggcga tgtacgggtt cttgtgcacg tcggcctcat ggccgggttt
     2941 cggcacaata ctcacccggt tttcccgcac taccgccgtc cggtttggaa atgagcggct
     3001 tgatacccgg tatacaccgc acaaacgact gtcctgggtc atggcaatgc ttacaatgcg
     3061 tcctacgtac atattcaccc ccaaagtaac tcgattccca tccacactac ttccattata
     3121 accaatccgg atgtgcgtgc gtattctcag cccaattccg tataatatcg ccggaaggag
     3181 ggatcgaccg tgcccatcac ccagcgggaa catgcccgca ggctcattac tcagttcaaa
     3241 ggggacgcct acatctacgg agtgcactgt ttcgaaaaac tcggggagca cgcccggcct
     3301 ctcggttctc gtgcggccgt ggtggcttcc gggtttggca aaccatgggg gccgcccctg
     3361 cacgaagcgg tgcgcaaatc actcgccggt gcgggtatta ccgcgggcga tatcattccg
     3421 ggtgcacggc ccaatgctcc ccgtgaagac gtcttccgca ttgtagaagc cctggaagaa
     3481 caggccccgg acctcgtggt cgctgtgggc ggcggcagca cgatcgacgc tgcgaaggcc
     3541 gccgtggccc ggctggcact cgcgcccgat caccccggtc tcgacgagta ttttgggtca
     3601 aatcaggtca cgcgcctgct cgcggatgcc gggaaacgca tgctcccagt gctggccgtg
     3661 cagctcgcct cgggttcggc cgcccatctc accaaatatt ccaacattac cgacgaggcc
     3721 agcgcgcaga agatgctcat cgtcgacgat gccatcgtcc cgccgcgctg cctctttgat
     3781 tatgcccaga ccacgaccat gtcgccggaa tttaccatgg acggcgccct ggacggcgta
     3841 tcgcattgcc tggaagtgtt ttacggcgcc cccggcgaag cgctcaatac cattcgcccc
     3901 gtggcgctgc tgggcatcga attgctcata cgccatgtga agcaggcctg tgcggaacca
     3961 gaggacctgg aggcgcgcga ggcgctcggg ctgggcaccg acctgggagg atacgccatt
     4021 atgctcggcg gcaccaatgg cgcgcacctg accagctttt cattggtgga tgtgctgcct
     4081 cacgggcggg cctgtgcgct catgaacccc tactacacgg tcttttttgc tccggcgatc
     4141 gaagcgcggc tgcgcgaagt gggggagatc taccgtgcag cggggtatac ccgccgtaac
     4201 cttgccggat tgcacgggcg cgacctcggc atcgccgtcg ctgaggccat gctcaacctc
     4261 tcccgcgaca tcggattccc ggttaccctc gacgaggtgc cgggattcag cccggagcat
     4321 attgagcgcg ccttgagcgc ggcccgggat ccgcggctcg caatgaaact gcagaacatg
     4381 cccgtgcctc tggccagtga ccaagtcgat gattacatgg gtcctgtgct cgaagccgcc
     4441 cggaccggag actttgagct tatacgaatc ttaaccgaat aaggagaccg ctgatgaaaa
     4501 ttcatttcat tctgggcatg gtgatcatag ctgcgctgtc cgcgaccgca tgggccgcca
     4561 gcggagcaga gggaggggcc agcacagtga acgtacagca cgtgaaaatc tattatgaaa
     4621 aaggccgttt cggcggatgg ccggccaatt acggcatgtg gaattggggc aacgaaatcc
     4681 tcgtgggctt ttcccgcggg tattacaagg atttggggcc gacccggcat catatcgacc
     4741 gcgagaaacc cgaggaacac tggcaggcac gcagcctgga cgggggcgaa acgtggtcgc
     4801 tcgaatatcc cgccgaacaa ggaagactca ttgcccaggg gacggcactg catggcatcg
     4861 aaacacccgg attgcctatc ccggaaatgc gcgagtgccc cggcgggatt gatttcagcc
     4921 atcccgattt tgccatgacc ctgcgcatga ccgacatcga tgcccgaagc ccgtcgaagt
     4981 ttttttacag ttacgaccgc ggccgcacct gggaagggcc gttcattctg cccaacgtcg
     5041 gagtcaatgg aattgccgca cggacggact atgtcgtcga cggcaaacac gagtgcatgc
     5101 tcttccagac cgcgggaaaa accgacggca ccgaagggcg cccgttctgt atgcgcaccg
     5161 tcgatggcgg gaaaacctgg gagtttctgt cgtggatcat gcccgagccc gagggattcg
     5221 gcattatgcc gtccacggtc cgcttgtcgg atgacaccct gctgactact atccgccgaa
     5281 gagagatctc aaaccgttgg atcgccgcct acatctcccg tgataatggc cgttcctggg
     5341 actttctcaa cgaccccgtg gacactctcg gcgaaggcaa tcctcccagc ctgattaaac
     5401 tctccgacgg ccgtctctgc ctgacctatg gcgttcgtat gcagccgttc cgcatcgccg
     5461 cgaaactcag caaggacaac ggtcagacct ggagcgatga gatcgttctt cgcgatgacg
     5521 gcgcggatcg cgacatcgga tatgtccgca gcctgcagcg gcctgacggc aaggtgatca
     5581 gcacgtacta catcgacgaa cccaccaccg gcccggagcg gtacatcgcc gccaccatct
     5641 gggatccggc ggaagtggag taaccgcacc cgcctgagac aaaacggccc ggaaagtccc
     5701 attggagctt tccgggccga tgctttttcg ccgggggccc ctacaccgtg taggtcgtcg
     5761 ctgccgtgtc gccgccgcgc ccggtccagt tggtgtggaa atactctccg cgaggcctgt
     5821 cgatccgctc gtaggtatgc gcgccgaaat agtcgcgctg cgcctggagc aggtttgcgg
     5881 gcagccgttc gtgccggtag ccatcgaagt aggccagtgc agtgctgatc gcaggcactg
     5941 gaatacccat cttcgccgcc tcgatgacca cgttgcgcca cgacgactgc gccgaggcga
     6001 cggcatcgcg gaagaacgga tccagcagca ggttgaccag gccggggtct ttgtcgaagg
     6061 cctctttgat ccgcccgagg aatacggagc ggatgatgca gccgccgcgc cacatcagcg
     6121 cgatgccgcc gtaattcagg ttccagccat aggcctttgc cgctgagcgc atcagctggt
     6181 atccctgcgc gtacgagaca atctttgccg catacagcgc cttgcgcaag tcgtctacaa
     6241 aggctttttt gtcgcccgaa aacggctggg tttcgccccg gagctgtttc gaagcctcaa
     6301 cacgctcgtc cttcagcgcc gacaggcagc gtgcgaacac cgcttcaccg atcaaggtga
     6361 gcggctggcc ttcatccagc gcggcgatgg ccgtccattt gcccgtgccc ttctggccgg
     6421 cggtgtcgag gatgaggtcc aggaccgcgt tgccgtcttc gtcgcggtag gcgaggatgt
     6481 cgcgggtgat ctctatgagg taggaattga gttcgccgcg gttccattcc gcgaacactt
     6541 cgtgcatttc gtcgttgctc atgccgagac cttccttcat catctgatag gtctcacaaa
     6601 tcatctgcat gtcgccgtat tcgatcccgt tgtggaccat cttcacgaaa tggccggcgc
     6661 cgttttcgcc gacccaatcg cagcacggct ctccttcatc cgtatgtgcg catatggtct
     6721 ggaagatctc cttcacgtgg ggccacgccg cgagcgaccc gcccggcatc atcgacggcc
     6781 cgttgagtgc gccctcttct ccacccgaca cccctgtgcc gatgtacagc aagcccttgc
     6841 tttccacata tttcgtgcga cggatcgtgt cggggaagtg gctgttgccg ccgtcaatga
     6901 tgatatcgcc cggatctaaa tgggggatga gctgctcgat cagcgagtct accgggtctc
     6961 cggctttaac cagcaacatg acccggcgcg gcttctgcag gttttgtacc agctcttcaa
     7021 tgctgtggca cccaacaatg ctcttgcctt ttgcgcgtcc ctggataaag ttgtccaccc
     7081 ggtcgcgggt gcggttgtac accgccacgc tgaatccctt gttggccatg ttgagcacga
     7141 gattttcgcc catcaccgcc agaccgatca aaccaatatc ttgctgtgcc atcgctcgcg
     7201 ttctcctcag acttctgtta accccatgca ctgttgaatc gggggtgttt tccagtgatt
     7261 ttgtgacatc gctacaatac cacaggccaa agagcgaatt cctctcgccc gcagccttcg
     7321 ggcgcggttc agtgccgccg gcagccgcac ccgtggatgg tgcattttgt gcaagcgcgg
     7381 aaacccgccg cgccgcccta tttgatcgcc acctctttgc ccttccggga agactcgtag
     7441 atggcatcga gaatctgctg aatacgcaag gattcctgcg gtctcacgcg cacaggcagg
     7501 ccgtcgcgca cgcattcggc aaaatgcagg atctccatcc ggtggccttc ctgttcagga
     7561 agccagtcgt attgcaggct tgtcagcgcg ccgtccgcag cgctggtgac aatcaacggt
     7621 tcagtgctga cgcccgcctt gtcgcccagc agccgcaacc cctggacatc ttttgggatg
     7681 ttcgcggccc agctcacctc cagattgagc gcgataccgt tttcgaaacg gatgaacgcg
     7741 gcggcatggt cttcgacgtc gaattcctcc acgggatacg gcacgcccca ctctccgttg
     7801 cacaggtctt tccggtcgcc gaacttgcgg ctcacctggc ccgtcgcgga cacgggtttc
     7861 gggcaaccca tcagccagac gatcagatcc agcacgtgca cgccgatgtc gatcagtggc
     7921 ccgccggcgg attcgcgctt gatgtgaaac ttgccccagc cgggaatgcc ccggcggcgc
     7981 aggt
//
LOCUS       DAOPBQ010000096         7958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8318_length_7958_cov_4.475009, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000096 DAOPBQ010000000
VERSION     DAOPBQ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8318_length_7958_cov_4.475009"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PKW60_04610"
     CDS             complement(<1..1087)
                     /locus_tag="PKW60_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98743.1"
                     /translation="MNPLGDKLRAGLLVLFAAAAPWDVFLFIPGLQIRLTSLLALAIL
                     VLEFIDYARTRRLGIRFELLWPACVLLLLSLPPARLVPVNGAAAILLGLAVARSAGTA
                     LARTIVAALALSVGLLALYSVVYFLMRFTTSAFMPPPSAYSLDTGLTAPFGHTVVECV
                     LLLFMGFAAAWSRFLRNDSVTGFLRRSLLAFAAPLLLALVILASLSLGAIRFWRPPDH
                     FASAGVTLAALTGGWLLARILAKSLLQWRDMRHTIAESTTLILSMLALFAVLFPLELR
                     LFWGFLAGTAAGAVQSERPLAPPPSNWALGFVPLALLVAVNINGVHPADRHNPRNYEI
                     AARQDFADADFTRLETRMDYVESAWPNE"
     gene            complement(1084..1236)
                     /locus_tag="PKW60_04615"
     CDS             complement(1084..1236)
                     /locus_tag="PKW60_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98744.1"
                     /translation="MMDAIQRMRRERRVALLLFAIFAFAALCVHGFSASAVRHAPRPV
                     PTETLE"
     gene            complement(1342..3888)
                     /locus_tag="PKW60_04620"
     CDS             complement(1342..3888)
                     /locus_tag="PKW60_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPJ98745.1"
                     /translation="MTKKLMVIPEEVRKKAVIDLGSIQVNAYTRTVTEEIASDSGVTP
                     AACLRIYRDMSIIREFENMLDSIKKLGSYQGIEYRHMGPAHLSIGQEGAAVGEAYHLN
                     VADHIYGSHRSHGEIIAKSLRAILELKGRGLREVMENYFGGAILSLIETHEPNWECLR
                     IDGKRAGKAKGGFASEEEEEIGIDFLLYGLLAEVFGRANGFNRGMGGSMHAFFIPFGV
                     FPNNAIVGGSADIAAGAALFKRVRRQPGIVVANIGDASIGCGPVWEGLQFASMAQFKN
                     LWEGEHRGGLPIIFNFMNNFYGMGGQPIGETMGFEYLSRAGAALNPDNMHAETVDGNN
                     PLAMADAYARKKAILEAGDGPVLLDVQCYRQTGHSPSDQSSYREREEIEMWRAVDPIT
                     EFGAKLIEAGVASQADLDTVQAYVTRKVTKACRLATDDSLSPRMTLTPHSGLTDIMFT
                     NVDEEELPGLERADDVLMPLEENPRVKQIAKKSRVGLDENGSIRKESKAVSLRDAIFE
                     AIAHHVYNDSRVVAYGEENRDWGGAFAVYRGLTEALPYHRLFNSPISEGAIVGTAVGF
                     ALEGGRPIVELMYCDFMGRAGDEVFNQLAKWTAMSGGYCRMPVVLRVSVGNKYGAQHS
                     QDWTALCAHMPGLKVCFPATPYSAKGLMAAALRGNDPVVYFESQRIYDITEIFHPGGV
                     PEEYYTVPLGVPEILKEGSDLTILTFGATLYRAYEAAQQLERDYGISVELIDGQTLVP
                     FDYEILYHSVKKTGKLILGSDACERGSYLHTIAANISQLAFDYLDAPPAIVGAKNWIV
                     PPAELEDEYYPLASSFLDVYHTQIKPLPGYEAREPRHPAELMRLSKEGML"
     gene            complement(4078..5202)
                     /locus_tag="PKW60_04625"
     CDS             complement(4078..5202)
                     /locus_tag="PKW60_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPJ98746.1"
                     /translation="MLPDTRSAILVVDDENGPRQALRMLLKEDHDVLLACNVPEAQEI
                     LAVQPVDVVITDLRMPKFSGVDLLKWIREFQPEIEVIILTGFGELETAMKAVAYRAFA
                     YLEKPFDNDTLLDHVNRALAQRRQDQERRRLQELALDANRFESVGRFVSGILHDLGTP
                     LSVIASQVDLIVPEEGLAALERRLNLIRSQSEHCNMLVRKAMGFLRHQTNAFSTLSLN
                     DVVESCLDVARPVLLRQGIQVEKSLAADIPACYGDLVLMRQALLNLIINASQAMESQQ
                     TPREIYVRTWATNGSVCLSIRDTGPGIPEKDRERIFEAFFSTKGNKGSGIGLAAVSDI
                     MSRHRGEAVLEDVDGPGASFRLTFPVTTPASQLHESSASA"
     gene            5886..6350
                     /locus_tag="PKW60_04630"
     CDS             5886..6350
                     /locus_tag="PKW60_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPJ98747.1"
                     /translation="MRRTRKVQSRESVIMSGFNAGFWEVPTSSRYLENIPTERNLWFE
                     TEQDRERRYALQDFFRSVLPAINTLIDTRLTERQRAILRLYYFKGMTQVEIAEQLHVT
                     QSTVSRHLFGTTRDGKKVGGAIAKLRKTLEKNGSQDVSLALKTLQGRMSQAS"
     gene            complement(6362..7258)
                     /locus_tag="PKW60_04635"
     CDS             complement(6362..7258)
                     /locus_tag="PKW60_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPJ98748.1"
                     /translation="MHWSRRQFFGSGLAAAAACALPGKVQAGEDSPAPRIGSKIGLSL
                     AAYSFRDYLPQGGKPGKITLHELCEMAAGWDLDAIELTSYYFSSEDSAYIHSLKAKAF
                     RLGLDISGMPIRNNFCLPPGEQRDAELQHVRAWVDHAVELGAPCIRIFAGQKNDPEKD
                     FAWMVEGMKASCDYAGSRGVFLAIENHGYLTDSAEALLRIVDTVDHEWLGINLDTGNF
                     REQPYENMAAVAHLALTVQIKIELRTHDGSGVENADYARIAAILRNAGYRGYAALEYE
                     GKEDPMTAVPPILATLRQTLAS"
     gene            complement(7541..>7958)
                     /locus_tag="PKW60_04640"
     CDS             complement(7541..>7958)
                     /locus_tag="PKW60_04640"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98749.1"
                     /translation="LDLYCGSGNFSLGWAHEGVEVLGIDRDSAAISCAAALGAGAYEV
                     GGEEAFAQALGRREWDTILLDPPRSGAKAIMPRLAQARAEAIVYVSCDAATLARDAAV
                     LCKAGWRIARTVIVDMFPNTWHVETVCRFEREAGSA"
BASE COUNT         1555 a         2456 c         2482 g         1465 t
ORIGIN      
        1 gttcgttggg ccaggcgctc tccacatagt ccatgcgcgt ttcgagccgg gtgaaatcgg
       61 catccgcgaa atcctgccgg gcggcgattt catagtttcg ggggttgtgc cggtcagcgg
      121 ggtgcacacc gttgatattg acggccacaa gcagggccag aggcacgaat cccagcgccc
      181 agttcgaggg cggcggcgcc agcgggcgtt ccgactgcac agcacccgcc gcggttcccg
      241 ccaggaatcc ccagaacaaa cgcagttcga ggggaaacag caccgcgaag agggccagca
      301 tcgagaggat cagggtggtg ctttccgcaa tggtgtggcg catatcccgc cattggagca
      361 gcgacttggc gaggatgcgc gcgagcagcc agccgccggt cagcgcggcc agcgtgacgc
      421 cggccgacgc aaagtggtcc ggcggccgcc agaagcggat ggcgcccagc gacagcgagg
      481 cgagtatgac cagtgccagc agcaggggcg cggcgaaggc cagcaggctt ctacgcaaaa
      541 accccgtgac gctgtcgttg cgcagaaacc gcgaccacgc agcggcaaat cccataaaca
      601 gcagcagcac gcattccacc acggtgtgcc cgaagggggc cgtcaggccg gtgtccagcg
      661 agtaggcgga aggcggcggc ataaacgcgg acgtggtgaa ccgcatcaag aaatagacga
      721 ccgaatacag agcgagcagc ccgaccgata aggccagtgc ggccacaatg gtgcgggcga
      781 gcgcggttcc ggctgagcgc gctacggcca gcccaagaag gatcgccgcg gcgccattca
      841 ccgggacaag tctcgctggc ggcagggaga gcaggagcag gacgcaggcg ggccacaaca
      901 attcgaaacg aatcccgagc cggcgggttc gcgcgtaatc gataaattcc agcacgagga
      961 ttgccagcgc aagcagcgag gtcaggcgga tctgcaggcc ggggatgaaa agaaacacgt
     1021 cccacggagc cgcggccgcg aacaagacca gcagtcccgc gcgcaatttg tctccaagcg
     1081 ggttcattcc agcgtttccg tgggaacggg ccggggcgca tgccggaccg cagacgcgct
     1141 gaatccgtgt acgcacagcg cggcgaaggc gaagatcgca aaaagcagca gcgccacccg
     1201 ccgttcacgg cgcatgcgtt ggatggcatc catcatggca gacttcccac gcgcatccgc
     1261 tgcgcgccct ggctctttcc cgtggcctgc gaagtatagc acgggcgcga aagggcccgg
     1321 cgcctcgtcc gcatcaccgg tttacagcat cccttccttc gagaggcgca taagctcggc
     1381 tgggtggcgg ggctcacgcg cttcgtagcc cggcaatggt ttgatctgcg tatggtagac
     1441 gtccaggaag ctggacgcga gcgggtagta ctcgtcttcg agttccgcgg gcggcacaat
     1501 ccagtttttc gcgcccacaa tggccggcgg agcatccagg tagtcgaagg ccagctgcga
     1561 gatgttcgcg gcgatcgtgt gcagatagct cccgcgctca caggcatcac tgccgaggat
     1621 gagcttgccg gtcttcttga ccgaatggta gagaatctcg tagtcgaacg ggaccagtgt
     1681 ctggccgtcg atcagctcta cggagatccc gtagtcacgc tcaagctgct gcgcggcctc
     1741 gtaggcccgg tacagcgtgg cgccaaaggt gagaatggtc agatccgagc cctctttcaa
     1801 gatctcgggt acgcccagcg gcaccgtgta gtactcctcc ggcacgccgc ccggatggaa
     1861 gatctcggtg atgtcgtaga tgcgctgact ttcgaaatac acgacggggt cgttgccgcg
     1921 caatgccgcc gccatcagcc ctttcgcgct gtacggggtc gcggggaagc acaccttaag
     1981 ccccggcata tgggcgcaga gggcggtcca gtcctgcgaa tgctgcgcgc cgtatttgtt
     2041 gccgacagat acccgcagca ccaccggcat gcggcagtag ccgcccgaca tcgcagtcca
     2101 tttggcgagc tgattgaaca cttcgtcgcc ggcgcggccc atgaagtcgc agtacatcag
     2161 ctcgacgatt gggcggccgc cttcgagggc gaaaccgacg gccgtgccca cgatggcgcc
     2221 ttccgagatc ggcgaattga acaggcggtg gtaggggagc gcctcggtca ggccgcggta
     2281 cacggcgaac gcgccgcccc agtcccggtt ttcttccccg taggctacca cgcgcgaatc
     2341 gttgtagacg tgatgcgcga tagcctcaaa aatcgcgtcg cgcaggctca cggccttgct
     2401 ttccttgcgg atactgccgt tctcgtccag ccccacgcgg gattttttgg cgatctgctt
     2461 gacgcgcgga ttttcctcga gcggcatgag aacgtcgtcc gcgcgctcga gcccgggcag
     2521 ctcttcctca tccacgttgg tgaacatgat gtcagtcagg ccgctgtgcg gcgtgagcgt
     2581 catgcgcggg gagaggctgt cgtcggtagc cagtctgcag gctttcgtga ctttccgggt
     2641 gacgtatgcc tggacggtgt cgaggtccgc ctgcgaagcc acaccggcct cgatgagttt
     2701 ggcgccgaac tcggtgatgg gatcgaccgc gcgccacatt tcgatctctt cccgctcgcg
     2761 gtacgatgac tggtcgctgg gcgaatgccc ggtctggcgg tagcactgca catccagcag
     2821 cacgggcccg tcgcccgcct cgaggatggc cttcttgcgc gcgtaggcgt cggccatcgc
     2881 gagcgggttg ttcccgtcca cggtctcggc gtgcatgttg tcgggattga gggccgcgcc
     2941 ggcgcgagag aggtattcga agcccatggt ctcgccgatg ggctggccgc ccatgccgta
     3001 aaaattgttc atgaaattga agatgatggg caagccgccc cggtgttccc cttcccacag
     3061 gtttttgaac tgcgccatgg aggcgaactg gaggccttcc cagaccggcc cgcagccgat
     3121 cgacgcgtcg ccgatgttgg ccacgacaat accgggctga cggcgtacgc gtttgaacag
     3181 ggccgcaccg gctgcgatgt cggcgctgcc cccgacgatg gcgttgttcg gaaacacgcc
     3241 gaaggggatg aagaacgcgt gcatcgagcc gcccatgccc cggttgaacc cgttggcacg
     3301 gccgaacacc tccgccagca gcccgtacaa caggaaatcg atcccgattt cttcttcttc
     3361 ctcgctcgcg aacccgccct tggcctttcc tgcgcgcttg ccgtcgatgc gcaggcattc
     3421 ccaattcggt tcgtgcgttt cgatcaggct gaggatcgcc ccaccgaaat aattttccat
     3481 gacctcgcgc aggcctctgc ctttaagttc gagaatggcg cgcaggctct tggcgatgat
     3541 ctcgccgtgg cttcggtggc tgccatagat gtggtcggcg acgttcagat ggtaggcctc
     3601 gcccaccgcg gcgccttcct ggccaatgga aaggtgcgcg gggcccatat gcctgtactc
     3661 gatgccttgg taggagccga gtttcttgat gctgtcgagc atattctcga attcgcggat
     3721 gatgctcata tcgcggtaga tgcgcaggca ggccgcgggc gtgacgcctg aatcactggc
     3781 aatttcttcc gtcactgttc gggtatacgc attcacctgg atcgagccca ggtcgatcac
     3841 ggcctttttg cgtacttctt cgggaatgac catcagcttc ttcgtcatgg ctctacacct
     3901 ttgactggta ggggtaccgt acgggagcag tcagtacaac aaatcggcgg ccaggcggcc
     3961 ggcttgaaac acgtccagcc aagtataccc caaactattg ggcgatttca tgcagagcgt
     4021 gcgcactcgc ccggcgcaaa acccgccggg gggcaaggtc tcgctgagga ggcaggctca
     4081 ggccgaggca gagctttcgt gaagctgaga tgccggggtg gtgacgggaa aggtgagcct
     4141 gaaactggcg cccgggccgt ccacgtcttc aagcaccgcc tccccgcgat gccggctcat
     4201 aatatcgctg accgccgcca ggccgatgcc ggaacccttg ttgcccttgg tgctgaaaaa
     4261 cgcctcgaaa atgcgttcac gatccttctc ggggatgccc gggccggtgt cgcgaatgct
     4321 caggcacacc gagccgttgg tggcccaggt gcgcacgtag atctcgcgcg gcgtctgctg
     4381 cgattccatg gcctgggacg cgttgatgat cagattgagc agagcctgcc gcatgagcac
     4441 gagatcgccg taacacgcgg gaatgtctgc ggcaagagat ttttcgacct gaatgccctg
     4501 acgcaacaga acgggccggg ccacgtcgag gcacgattcc accacgtcgt tgagggaaag
     4561 ggtggagaag gcgttggtct gatggcgcag aaagcccatg gctttccgga ccagcatgtt
     4621 gcagtgctcg gattgtgatc ggatgagatt gagtcgccgc tccagtgccg ccagcccctc
     4681 ttccggcacg atgagatcga cctggctggc gataacggac aggggcgtgc ccaggtcgtg
     4741 cagaattccc gagacaaaac gccccaccga ctcaaaacgg ttggcgtcca gggccagttc
     4801 ctgcagccgg cggcgctcct gatcctgccg ccgttgcgcc agcgcccggt tcacgtgatc
     4861 caggagcgta tcgttatcga agggtttttc aagataggcg aaagccctgt acgccacggc
     4921 tttcatggcg gtctccagct cgccgaatcc ggtcaggata atgacctcga tctcgggctg
     4981 gaactccctg atccatttca gcaggtcgac cccggaaaac ttgggcatgc gcaggtcggt
     5041 tatgaccacg tcgacgggtt gaaccgccaa aatttcctgc gcctcgggca cgttgcatgc
     5101 cagcaggaca tcgtgatcct ctttgagcag catgcgtaat gcctggcggg gcccgttttc
     5161 gtcatcgact acgagaattg cacttcgtgt gtccggtagc actgcagcac ctctgtacgt
     5221 ttccccgatg cgacccttcc actccctcgg gacctgggta tcgattatcc cccattgccc
     5281 tcgaaatgcg ctgtggtagc gctgcaaaaa cgatgcgtcg accgctttta aagtgcaacc
     5341 actgtgccaa cgtcaacaat ttaccttaat ccgctgaaaa tgtaaaggtt gtgaaaataa
     5401 ttttcggcgg cgcagtgtac aaaaatgaac atcgcggcct tcttcgccgg tttctgttca
     5461 aaattgaacg ccactctcga cgggatttac ccaaaaagta ggcataaggg cgggtcacat
     5521 aagcacagcc gccgttgtta cctgaaggtg gcggtgttgt ttttgcatgg cccgtatggt
     5581 attacatgca cgtacgaggc gaaaacggga aaggaaacgg ctttttccgg aaccgtttcc
     5641 agtaagaggt caaaacgaac aatgcgcaag cgtaacgcga taaccacgcc ctggccggcg
     5701 gcgggcagcc ctgccgctcg actcgcgtgg cgtattattc gcgctggggt tcgtcctcgg
     5761 aggagtctcg tatagcatag acggacaaac agagaagtca gagacgccgc aggcgaaccc
     5821 gaaagggcct gcggcgtttt tctttttccg accgtgcctg tgtacgcggg cccccgcgtt
     5881 cacggctgcg ccgcacaaga aaagttcaat ccagggagtc tgtaatcatg tccgggttta
     5941 acgcgggctt ctgggaagtt cccacaagct ccaggtacct tgaaaatatc cccacagagc
     6001 gcaatctgtg gtttgagacc gagcaggacc gcgagcggcg ctatgcgctg caggactttt
     6061 tccgcagcgt cctgccggcg atcaacacgc tgattgatac ccgccttacg gagcgccagc
     6121 gggccatcct gcgcctgtat tacttcaagg gaatgacgca ggtggagatc gccgagcagc
     6181 tccacgtgac gcagtccacc gtcagccgcc acctgttcgg cacgactcgg gacggcaaga
     6241 aagtgggcgg cgcgatagca aagctgcgca agacgctcga aaaaaacggc agccaggacg
     6301 tgtcgctcgc gctgaagacc cttcagggcc ggatgtctca agcgtcctga gctgttcccg
     6361 ctcaggaggc cagggtctgc cgtaacgtcg cgagaatcgg cgggaccgcg gtcatcgggt
     6421 cttctttgcc ttcgtattcc agggcggcat agccccggta tccggcgttt cgcaggatgg
     6481 cggcgatgcg tgcataatcg gcgttttcca cgcccgagcc atcgtgcgta cgaagctcaa
     6541 tcttgatctg cacggtcaac gccagatgcg ccacggcggc catgttttca tacggctgct
     6601 cgcgaaagtt ccccgtgtcg agattgatgc cgagccattc gtggtcgacc gtgtccacga
     6661 tgcgcagcag ggcctctgcg gaatccgtca ggtagccgtg attttcgatg gccagaaata
     6721 ccccgcgcga gcccgcataa tcgcaactgg ccttcatgcc ctccaccatc caggcaaaat
     6781 ctttctccgg gtcgtttttc tgcccggcaa agatacggat gcagggcgcg cccagctcca
     6841 ccgcatgatc gacccaggcc cggacatgct gcagctccgc gtcgcgctgc tcacccgggg
     6901 gaaggcaaaa attgttgcgg atgggcatcc ccgagatgtc cagtccgaga cgaaacgcct
     6961 tggccttcaa cgaatggatg tacgcggagt cctccgacga aaaatagtac gaggtcagct
     7021 cgatggcgtc cagatcccag cccgcggcca tttcgcagag ctcatgcagc gtgatcttgc
     7081 ccggtttgcc gccctggggc aggtaatcgc ggaatgaata cgccgccagg ctcaacccga
     7141 ttttcgagcc gatccgcgga gccggggagt cttctcccgc ctgtaccttg ccgggcagcg
     7201 cacaggctgc cgctgctgcc agaccagaac caaaaaactg ccgccgtgac cagtgcatgg
     7261 gagactcctt tgcgaattca gcttgcctta gtttttgcgc cagcagcgca ggcggtgcag
     7321 cacttcgcgc gcggctggca tcacgaaaac cgcctgcacg cacgcaaaac acgcacgatc
     7381 ggccaaacac cgcctttccg gcatagtata cgcacatttt gacggtttta aagggccgat
     7441 cgcagaaaaa ttgcgcgaaa agccgccgcg ccgtccagat cagcgccggt tgagacctcg
     7501 agcggcaaca gcggccgccc gtacgagatg cattcggtgc tcaggctgac ccggcctcgc
     7561 gttcgaagcg gcagacggtc tcgacgtgcc acgtgttggg gaacatgtcg acgatgaccg
     7621 tgcgcgcaat gcgccacccg gctttgcaca gcaccgcggc gtcacgggca agcgtggccg
     7681 cgtcgcagga gacatacacg atggcctccg cgcgggcttg agccaggcgc ggcatgatgg
     7741 ccttcgcgcc ggagcgcggg ggatcaagaa ggatcgtgtc ccattcccgg cggcccaggg
     7801 cctgcgcaaa ggcctcttca ccgcccacct cgtaggcgcc ggccccgagg gcggccgcgc
     7861 agcttattgc ggcggaatcg cgatcgatgc ccagcacctc gacgccttcg tgcgcccagc
     7921 cgagcgaaaa gttgccgctt ccgcaataga gatcaagc
//
LOCUS       DAOPBQ010000097         7843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8450_length_7843_cov_3.546225, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000097 DAOPBQ010000000
VERSION     DAOPBQ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8450_length_7843_cov_3.546225"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKW60_04645"
     CDS             <1..408
                     /locus_tag="PKW60_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98750.1"
                     /translation="VGGLYRNTKQKSLETVPWLTQAEDVAVGVAENFIPGNFSGAPLS
                     TTFGHRETDEGRRELVFFIKSELWRAPFVVADEHGFVDLREETPATKISPTGLLTDIL
                     EGVSSIPQNLSEAITGGSSSTGINSELKMEDNK"
     gene            408..1487
                     /locus_tag="PKW60_04650"
     CDS             408..1487
                     /locus_tag="PKW60_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HPJ98751.1"
                     /translation="MGIQRLVPALALLGIVSGLALAGEETPPPPPIEPPPAPQLVEAI
                     RQVQIQVWISETTEDGLRDIGTNLDYTRFVRGNENSGSVERISTRVFDPNSFQVTLPA
                     ADTNPYPDNVRLTPDFPARLADGSVLQGEAITTQSGAGITFSIIDSGTGTIDGIIRGI
                     EQKADADLISKPELLVVENGTAEIHAGEEIPYQSLIYQKGRAQLQVTWRQVGVNLSIQ
                     PVILDDDMIEINLAKLNVVDRLPSSPIRGIDLPVFSTREQTGQVLVPNGQTLVIGGLS
                     TRNVTKTERRIPLVGKLPVLGAPFRGRRVEARNSHLLIFLSPTIVDLRNLRPEAISAL
                     NFWREEKWKNLDRIEQEIEIMEDEL"
     gene            1663..3201
                     /gene="lnt"
                     /locus_tag="PKW60_04655"
     CDS             1663..3201
                     /gene="lnt"
                     /locus_tag="PKW60_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPJ98752.1"
                     /translation="MLSGVLLALCFPTWQLFFLAWVALTPLFYVCRNTTPRDTALRFF
                     VAGWAFHSVVLQWLLTNIYWAGGWAFWGQQAVCLVMAAYWAVTGATWAWLRNQLRWMP
                     QSLLLAVLWASMEYAQNHLLSGFGWSNLAYSQGRDLLLLQLAAIGGTPLIAGLLVACN
                     VLFSELLHTRRLRFARAALALALLVGSHGVGMALLQPADYASPPFQAGVFQSNFPLEM
                     KWDPEYSLEMVRNAAEKSRLLADHAPVHLFVWPETLVMDDLTAPGILAPLEELTRSTG
                     AALYTGSQRTDAETGRARNSSFLISPSGEVEGYYDKIHLAPFGEYVPFGSYFPFIQKI
                     VPIISDVEPGRETRVFSLEERTFGPLICFEVLFAEMSEALRRQGADFLVVITNLGWFG
                     ASNALEQELEQARLRAIETRLAVVHCANTGLSGVFDPWGRFAATNAVVMPSGAFHWLR
                     EDQSARDMKQYRCLGALPVAEPAARPVPWAPQRVPQASVAASAVLVVLALAARHPSSA
                     GQAP"
     gene            3230..4837
                     /gene="murJ"
                     /locus_tag="PKW60_04660"
     CDS             3230..4837
                     /gene="murJ"
                     /locus_tag="PKW60_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPJ98753.1"
                     /translation="MNQKAHSSARVSARLASIFAGGTMLSRVLGLLRDMVVGALVAAG
                     SRDAFILAFKLPNMLRDLIGEGALNAAFVPVYTKTLEQDGDQEFKKLVSSAMASMLVV
                     LLVLTLLGTALVPVLLGGLNALKPITGGKTLSPEDLGLITVLARWAFPYLFFIGMAVF
                     AMGPLYTLREYATPSWSPALLNIALIVCCALFRHSFPDPSYALILGVWLGGLAQLAVL
                     YVSLGRKTGIWLPDFRLRHAGVWKIYALMLPVLIGQAAGEVNKLVDALFARSLDEGIV
                     SAFYYANRLIQLPLSMFGFAVAVAILPLLSQAAARNRVKEIRETLMTGFRQSYFLVCP
                     SLLALLFFGKPLVRLLFERGDWTAAETDLTVGALSIYSLGLLSFAWIKIGVTGFFCVG
                     DTKTPVVAASASMLLNILLNIAMVKPLGYRGLALATVISYTVNFGGLYYLLVMRHGPL
                     WNSAFFTALLRMTVAGAVMTAAGYAAYTRVLALWPADSGIARLASVSALLAASAAGYL
                     GACHVLRVKELNDFTSMLRLKTRGERA"
     gene            5249..6244
                     /locus_tag="PKW60_04665"
     CDS             5249..6244
                     /locus_tag="PKW60_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98754.1"
                     /translation="MAKYNYKAIDGDGNETSGVIEAETENDALSLIGRQGLYVTSIHK
                     THIGTMLRQRWVEQKELRKKYIEQKRTEARRKHPRQRLVVRFKDGTTNYGVCFALNIQ
                     DAGFYLDTMTREGAATGETVQIRFNQLKAVFQVKSFDGHFDPQQRYPEYHPEGGEYIC
                     EFEDGEVIRGVTLLPYEPEAERFYLIPVDPASNNISILVERSALVHVYTPETYEQKQA
                     RESEERRKEAAVAGMTHEESMGDFYFETRNYPAALEQYKAAVKAQPRSPRLRKKILAT
                     EYNIAVQFIKRRDYETALAGMLKILEMDPSNPHARKKVSQLRRIIEKDKSRQSSE"
     gene            6290..7021
                     /locus_tag="PKW60_04670"
     CDS             6290..7021
                     /locus_tag="PKW60_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPJ98755.1"
                     /translation="MLIAIISDLHANLEATLAVFKRIDERKPDRIICLGDLTGYNANP
                     NEVVDIIREREIPTIMGNHDAAVCGLEDPWFFRAAAKKAIEWQYEQIRDDNRRWLASC
                     HEQIVFNSDCLGVHGSPSNRDDYIIDWLDAVRQLEYLNGRDINICFFGHSHRASFFSE
                     KGNTGTTPAEGVCQLSPMNRYFINPGATGQPRDRDPRAAFGLFDTDKRTFEFCRAEYD
                     IDACALKIMQAGLPIELARRLFKGK"
     gene            7032..>7843
                     /locus_tag="PKW60_04675"
     CDS             7032..>7843
                     /locus_tag="PKW60_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HPJ98756.1"
                     /translation="MTVREQIEQRERQTLSPHAMLVANTRGRDVYEPEHPFRTAYQRD
                     RDRIIHSKAFRRLKQKTQVFLAPERDHYRTRLTHTLEVAQIARTAARALFLNEDLAEA
                     IALAHDLGHAPFGHAGEYVLNELYSEGFNHTAQSVRIVEKLECTRRGPGLNLTFEVRD
                     GIANHSKDKGILWGDTTRTSATREGQIVAICDAVAYINHDIDDAMRGGILRPSDLPAT
                     ALKHLGETSSERIDGMVRGIIEGSIDGEIGLVDAVLESTIQLRDFLYREVYP"
BASE COUNT         1549 a         2229 c         2463 g         1602 t
ORIGIN      
        1 gtcggcggtc tctaccgcaa caccaaacag aagagtctgg agacggttcc gtggctgacc
       61 caggcagaag atgtggcggt cggcgttgcc gagaacttca ttcccggcaa tttcagcggc
      121 gccccgctgt cgacgacgtt tggccaccgg gagacggacg aaggccgccg cgagctggtt
      181 ttctttatca agtcggaact gtggcgggcg ccttttgtag tggccgacga gcatggtttt
      241 gttgatctga gagaagagac cccggcaacg aagatatcac ccacgggttt gctgacggac
      301 atcctggaag gggtgtcatc catcccgcag aacctcagcg aagcgataac aggcggttcg
      361 tcgagcacgg ggatcaattc cgagttgaaa atggaggata acaagtaatg ggtatccaac
      421 gccttgttcc agcgcttgcg ctgctcggaa tagtgtcagg cctcgccctg gccggcgagg
      481 agacccctcc cccgccgcca attgaaccac cgccggcccc gcaactggtc gaggccatcc
      541 gccaggttca gatccaggta tggatcagcg agaccacgga agatgggttg cgggatattg
      601 gaaccaatct ggactatacc cggtttgtcc ggggcaacga gaacagcgga agcgtagagc
      661 ggatttccac gcgggtgttc gacccgaatt cttttcaagt gactctgccg gccgcagaca
      721 ccaacccgta cccggacaac gtgcgcctga caccggattt tcccgcccgg cttgccgacg
      781 gcagcgtcct gcaaggggag gccatcacaa cacagagcgg cgcgggaatc acgttcagca
      841 tcatcgacag cggcacggga acgattgacg gcatcatccg cggcattgag caaaaagcgg
      901 acgccgacct gatttcgaaa ccggaattgc tggtggtgga gaacgggacc gcggagatcc
      961 acgcgggcga agaaatcccc taccagtcgt tgatttatca gaaagggcgc gcgcagctcc
     1021 aggtgacgtg gcgccaggtc ggtgtgaacc tgagcattca accggtgatc ctcgatgacg
     1081 acatgatcga gatcaacctg gcgaaactga atgtggtcga ccggctgccg tcgagcccca
     1141 tccgcggcat cgatcttccc gtgttctcca cgcgtgagca gacgggccag gtgctggtgc
     1201 ccaacggtca gacgctggtg ataggcggcc tgtcgacgcg caatgtcacc aagaccgagc
     1261 gccgcattcc cctggtgggc aagctgccgg tgcttggcgc ccccttccgc gggcggcggg
     1321 tggaagcgcg caattcgcac ttgctgatct ttctctcccc gaccattgtt gatcttcgca
     1381 acctgaggcc ggaagccatc agcgctctca acttctggcg tgaagagaaa tggaagaatc
     1441 tggaccgcat cgagcaggag attgaaatca tggaggacga gctgtagctt gttctcagtg
     1501 gatgaggcaa ctcagcaagc tcccggcagg ccagatgtct ggcctgccgt tttttatgcg
     1561 agccgcaccg gagcgggtcg gattttggag ttgaattgag cgcccggcca tgcgcatgat
     1621 cccgccacga tgaagacgat attccgacaa gggggagccc cagtgctgag cggtgtgctg
     1681 ctcgcgctgt gtttccccac gtggcagctg ttttttcttg cgtgggtcgc gctcacacct
     1741 ttgttttacg tgtgccgcaa caccactcca agggacaccg cgttgcgctt ctttgtggcc
     1801 ggctgggcgt ttcacagcgt ggtgcttcag tggctgctca cgaatatata ttgggcaggc
     1861 ggctgggcgt tctggggcca gcaggcggtt tgtcttgtaa tggcggcgta ctgggcggtc
     1921 acaggcgcca cctgggcctg gctgcgaaac cagctccgct ggatgccgca atcccttctg
     1981 ctcgccgtgc tgtgggcgtc catggagtat gcgcagaatc atctgctcag cggttttggc
     2041 tggagcaacc tggcctattc gcagggcagg gatctcctgc tgctgcaact cgccgctatc
     2101 ggagggacac ccctcattgc cgggctgctt gtcgcgtgta acgtgctgtt ctcggagctg
     2161 cttcacacgc ggcggctccg gttcgcgcgc gcagcgctgg cgctggcact cctggttgga
     2221 agccatggcg tgggcatggc cctgctgcaa ccggccgact atgcctctcc cccattccag
     2281 gcgggcgtgt tccagtctaa ttttccgtta gagatgaaat gggaccccga gtacagcctg
     2341 gagatggtcc gcaacgccgc ggaaaagtcg cgcctgctgg ccgatcacgc gcccgtgcac
     2401 ctgtttgtct ggccggagac cctggtgatg gatgatctga ccgccccggg gatcctggct
     2461 ccgcttgagg agctgacgcg gagcaccggt gccgcactct acaccggctc gcagcgcacg
     2521 gatgcggaaa ccgggcgggc ccggaactcc agcttcctga tttcgccatc cggcgaagtc
     2581 gaggggtatt acgacaagat ccatctggcg ccgtttggag aatacgtgcc gttcgggtct
     2641 tatttcccct ttatccagaa gatcgttccc atcatcagcg atgtggaacc cggccgggag
     2701 acgcgggtct tttcgctcga agaacggaca ttcggcccgc tgatctgttt tgaggtgctc
     2761 tttgcagaaa tgagcgaggc attgcgccgg cagggcgccg atttccttgt ggttatcacg
     2821 aatctgggct ggttcggggc aagcaatgcg ctcgaacaag agcttgagca ggcgcggctt
     2881 cgcgcgatcg agacccgcct ggcggtggtt cactgcgcaa acaccggatt atccggcgtg
     2941 tttgatccct ggggccgctt tgcagccacg aatgcggtgg tgatgccgtc tggagcgttt
     3001 cactggcttc gagaagacca gtcagcgcgg gatatgaagc aataccgttg cctgggcgcg
     3061 ctgcccgttg ctgaaccggc ggctcgcccc gtaccgtggg cgccccagcg cgttcctcag
     3121 gcgtccgttg cggcgtcagc cgtcctggtg gtgctggcgc ttgccgcccg ccacccgtct
     3181 tcggccggcc aggcgccatg aagagatgcg gggccacaga cagaaaacca tgaatcaaaa
     3241 agcacactct tcggcgcgcg tgagtgcgcg cctggcgtct atcttcgctg gaggcacgat
     3301 gctgagccgc gtcctgggcc tgctccggga tatggtggtg ggggcgctgg tggcggccgg
     3361 ctcgcgggat gccttcattc tcgcattcaa actgcccaat atgctgcggg acctcatcgg
     3421 cgagggggct ctgaatgcgg cgtttgtacc ggtgtacacg aaaacactgg agcaggatgg
     3481 agaccaggaa ttcaagaagc tggtgtcctc agccatggcg tccatgctgg tagtactcct
     3541 ggtgctgacc ctgttgggca cggcgttggt gccggtgctc ctgggcggac ttaatgcgct
     3601 gaagcccatc acgggaggga aaacgctgtc cccggaggat ctgggactga tcacggtgct
     3661 tgcccggtgg gcgtttccgt acttgttttt catcgggatg gctgtattcg cgatggggcc
     3721 gttgtacacg ctgcgcgaat acgccacgcc ctcctggtcg cccgccctgc tgaacatcgc
     3781 gttgattgtc tgttgcgccc tgtttcgcca tagttttcca gacccgtcct atgcgctgat
     3841 tctgggcgtc tggctgggtg gcctggcgca actggcggtg ctgtatgtgt ccctgggccg
     3901 aaagacaggc atctggcttc ccgatttccg gctgcgtcat gccggggtct ggaagatcta
     3961 tgccctcatg ctgccggtac tgatcggaca ggccgcaggt gaagtcaata agctggtgga
     4021 tgcgcttttc gcccgcagtc tggatgaggg cattgtaagc gcgttctatt acgcaaaccg
     4081 cctcattcag ctccctcttt cgatgtttgg tttcgccgta gccgtggcga ttctgccgct
     4141 gctttctcaa gcggcagccc ggaaccgggt gaaggagatc cgggaaacgc tgatgaccgg
     4201 gttccggcag agttattttc tggtctgccc ctccctgctg gccctgttat ttttcgggaa
     4261 accgctagtt cgcctgctct ttgagcgagg agactggacc gccgctgaga cggacttgac
     4321 ggtgggggcg ttaagcattt attcgctcgg ccttctgtcg tttgcgtgga tcaagattgg
     4381 tgtgacggga tttttttgcg tcggcgatac caaaacgccc gtggttgcag ccagcgcgag
     4441 catgctgttg aatatccttc tgaatatcgc aatggtcaag cccttggggt accgtggtct
     4501 ggccctggcc acggtgatct cgtataccgt gaacttcggc ggcctgtact acttactcgt
     4561 gatgcgtcat ggtccgctgt ggaacagcgc gtttttcacg gcgctgctcc gcatgactgt
     4621 cgccggcgcc gtaatgaccg cagcgggata tgccgcgtat acccgtgttc tggcgctgtg
     4681 gccggcggac tcgggcatcg cacgtttggc gtcagtgagc gcgctgctgg cagccagtgc
     4741 cgcgggatac ctgggagcct gccacgtctt gcgggtgaaa gaactgaacg acttcacttc
     4801 catgctgcgg cttaaaacac gcggagaacg ggcgtaaggg ccgcgcggcg cgaccaaacg
     4861 tcggcgcttg agcggaccag acgttggcgc attcgtacag cggcccttcc ggcacgcccg
     4921 gaggcgcctc ccgcctggaa cccggtgtga atgccccggc agagcctgct tgtaatcgct
     4981 acggggatgg cataaaatgg aactcgaaaa gctcgcgaga gcgcctttgg gcggtccagg
     5041 agtctgccga agaatcgctt ggggtgagag tgcggcagaa gcttagcact atcaacgggt
     5101 taaatacttt taaccctttg acaggcccgc gatttcgtgt taaggtttcg aattgaagag
     5161 tcgtggatgt ctgcttttga gccacgcggt ttcgattcga atcgaacgca acggcggtgc
     5221 acgcagctac gcctgaggag acggggcagt ggcaaagtac aattacaaag cgatcgacgg
     5281 cgatggcaac gagacttccg gggtgattga agccgagact gaaaacgacg ccttgagcct
     5341 gattggccgc caggggctgt atgtgacgtc aattcacaaa acgcacatcg gcaccatgct
     5401 gcgccagcgc tgggtcgagc agaaggaact gcgcaagaaa tatatcgagc agaagcggac
     5461 tgaggcgcgg cgcaagcatc cgcgccagcg ccttgtggtg cgttttaaag atggcacgac
     5521 caattacggg gtttgttttg cgctgaacat ccaggatgcc gggttttatc tggatacgat
     5581 gacgcgcgaa ggcgccgcca cgggggagac ggtgcagatc cggtttaacc agctgaaggc
     5641 tgtatttcag gtcaagagct ttgatgggca ttttgacccg cagcagcgat atcctgagta
     5701 ccatccggag ggtggcgaat acatctgtga atttgaagac ggcgaggtga tccggggcgt
     5761 tacgctcctg ccgtacgagc cggaggcgga acgtttttac ctgatccccg tggatccggc
     5821 aagcaacaat atttcgattc tggtggagcg ttcggcgctg gtccatgtgt acacaccgga
     5881 gacctacgag caaaaacagg ctcgggagtc cgaagaacgg cgaaaggaag ccgcggttgc
     5941 ggggatgacg catgaggaat ccatggggga tttctacttc gaaacgcgca actatcccgc
     6001 ggcgctcgaa caatacaaag ccgctgtgaa agcccaaccc cgttcgcccc gccttcgaaa
     6061 gaaaatcctg gcaacggaat acaacatcgc ggtgcagttc attaaacgcc gcgattacga
     6121 gacagcgctg gctggcatgc tgaaaatact tgagatggat ccgtccaatc cgcatgcccg
     6181 caagaaagtg tctcagttgc gccggatcat tgaaaaagac aaatcccggc aatcatcgga
     6241 gtaggcactg ccgcaaccaa gtagagcgat ttccctcaag gacgagtaga tgttaattgc
     6301 gatcatatcg gatcttcatg ccaatctgga ggcaaccctg gcggtattca agcgtatcga
     6361 tgagcggaaa ccggaccgta tcatctgcct tggcgacttg acggggtaca acgccaatcc
     6421 caacgaagtg gtcgacatta tacgggagcg ggagatcccc accatcatgg ggaatcacga
     6481 tgcggcggtg tgcggactgg aagacccttg gtttttccgg gccgcggcca agaaggcaat
     6541 tgaatggcag tatgagcaga ttcgcgacga taaccggcgc tggctcgcca gttgccatga
     6601 acagattgtg ttcaactcgg actgtctggg ggtgcacggt tccccgagca accgcgacga
     6661 ttacatcatt gactggctgg atgccgttcg gcaactggag tatctgaacg gccgcgatat
     6721 caatatctgc tttttcggtc acagccatcg cgcctcgttt ttcagcgaga aaggcaacac
     6781 agggaccacg cccgccgaag gcgtatgcca actcagcccg atgaaccgct atttcatcaa
     6841 cccgggtgcg accgggcagc cgcgcgaccg cgacccccga gctgccttcg gcctgtttga
     6901 cacggacaag cggacctttg agttctgtcg cgctgaatat gacatcgacg cctgcgcgtt
     6961 gaagatcatg caggcgggtt tgcccatcga actggcgcgc cgtcttttca aagggaagtg
     7021 agcgccagca cgtgactgtc cgtgagcaaa ttgagcagcg ggaacggcaa accctgagcc
     7081 cgcacgccat gcttgtagca aacacgcgcg ggcgggatgt ctacgaaccg gaacatccgt
     7141 ttcgcacggc ctaccagcgt gatcgcgacc ggatcatcca ctcaaaggct tttcggcggc
     7201 tcaagcagaa gacacaggtg tttcttgcgc cggaacggga tcactaccgc acgcgcctga
     7261 ctcacacact cgaggtggcg cagattgcgc ggacggcagc ccgtgccttg tttctgaacg
     7321 aagacctcgc cgaagccatt gcgctggcgc atgacctggg ccacgcaccg ttcgggcatg
     7381 cgggcgaata tgtgctgaat gagttgtatt cggaaggttt caaccatacg gcacagagcg
     7441 tgcgcatcgt cgaaaagctg gagtgcaccc ggcgagggcc cggattgaat ctgacattcg
     7501 aggtgcgcga cggcatcgcg aaccattcga aagacaaggg cattctgtgg ggcgacacaa
     7561 cgcgcacctc ggcgacccgg gaaggccaga tcgtggccat ctgcgatgcg gtcgcgtaca
     7621 ttaaccacga cattgacgac gcaatgcgag gcggcatcct ccggccatcg gatctccccg
     7681 cgactgccct gaaacatctc ggagagacgt cgtctgagcg cattgacggc atggtcaggg
     7741 gcatcatcga aggatcgata gacggcgaga tcggcctggt tgacgcagtg ctggagagca
     7801 cgattcagct gcgggacttt ctgtatcgcg aagtgtaccc ctg
//
LOCUS       DAOPBQ010000098         7838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8458_length_7838_cov_4.373378, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000098 DAOPBQ010000000
VERSION     DAOPBQ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8458_length_7838_cov_4.373378"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3009
                     /gene="drmD"
                     /locus_tag="PKW60_04680"
     CDS             <1..3009
                     /gene="drmD"
                     /locus_tag="PKW60_04680"
                     /inference="COORDINATES: protein motif:HMM:NF038317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DISARM system SNF2-like helicase DrmD"
                     /protein_id="HPJ98757.1"
                     /translation="ALVRAARWTAITPYMDPDDQGPIDRLPVCSPFHGSIQVEDYQLV
                     PLLKALRMPRVNLLIADDVGLGKTIEAGLILSELLIRRRINRILILTPASLRIQWRDE
                     LWSKFSLPFDVIDHDFTLNLKRSLGVDANPWRCSSRAIASYHYLRQPDIREQFESACR
                     TSEGSPHLAWDLLVVDEVHNLMPSPFGEDSLLCQMLRRIAPQFEHRLFLTATPHNGHT
                     RAFSGLLELLDPVRFSRTDELRPAERERIKQIVIRRLKREVNERTSPPRFCTRRPPEA
                     LVLKFSKEEQGLIDAFEAFREKVRSLIAASSRKRRLAGRFAIEILGKRMLSGPVTFLE
                     SWRRCKIGLQLEDPANDDDLRAAGEAANEETADDREAEQRGATAAAVIGAWMQPLAKD
                     LEAEIARIDAAAKHMGISLDNEVVSQNPAGDARFEVLRWFIDQRLRQESNWRDDERLV
                     LFTEYKTTLDYLLRRLRESFPGQEDRFLSLFGGMDDINREAIKDAFNDPDHRVRILIA
                     TDAASEGLNLQSTARYVLHFDCPWNPARLEQRNGRLDRHGQARDVHVFHFASEQTADL
                     KFLAYLIHKVHQIREDLGATGELFDEATHKCLVLGEEPAAVQRTLDIQIEHTKGATQM
                     DADDTVSEDSSHGESGLSDKLNALADALDLDPVSGHATLEAAMAADGGRPQLTVPDDT
                     LRFSLINPHLLGWKDTIDETIRKRTGQGLLGPVPRLTFSVKPFMVPVGERTVFRPRVD
                     TLMLHLAHPLMQQATGRLTRHRFPGPNAVSRWTVKLGDVPADADALILLHVEELAVNQ
                     LRETFHHWVRTVAIPVADGELRKPLAPMPAITLRGAQPCRAPELRAQAASLLSDLEPE
                     LQEFVKKQRRELTANLQKQLEIDREKALEEEERRFQSRQGEVSTLIAENTLAKLQRQI
                     DRLKRMSQQGMLFEQQAYCDEIDRSIELKQEELLRRKQHYEEVRQQLAQERERIVRRL
                     LPKRFTLQGEAQVFPVAVEVRFPREQGGAK"
     gene            complement(3420..4001)
                     /locus_tag="PKW60_04685"
     CDS             complement(3420..4001)
                     /locus_tag="PKW60_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98758.1"
                     /translation="MIGTLIDEAEKGQDARPGAAIGHMPGLAAAIKQLAQPLKAVGGV
                     KDRVVPRQKPFAALREEDNDHAHDDTHGGPVNVLRIELVATEVERGRVAVYEQFSGLS
                     NAFAQDFGQIGLAFAAVVDRRQQGTGLAFFWRHPQRGGYQRAKGIEFVRKPAFCLPLF
                     EFPFSPGVVVEAGKDYAPLMAVGDQSELFIALS"
     gene            complement(4062..4307)
                     /locus_tag="PKW60_04690"
     CDS             complement(4062..4307)
                     /locus_tag="PKW60_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98759.1"
                     /translation="MTVHAAAEFKIQKTFVENADVLAVETGEVDRRHHPRTGPALADR
                     NSRSCEHVEQLPHQRIIHDVLLETGIFKNINRPFHTV"
     gene            4987..5307
                     /locus_tag="PKW60_04695"
     CDS             4987..5307
                     /locus_tag="PKW60_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015161441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPJ98760.1"
                     /translation="MDLVTLVYKLTASFPKAEIYALTNQLRRAAVSVPSNIAEGQGRR
                     STKEFLNHLSMAKGSLMEVETQTAIALRLKYLKQEKQEQVKALTLVTGRLLNGLIRAL
                     RHKL"
     gene            complement(5469..6023)
                     /locus_tag="PKW60_04700"
     CDS             complement(5469..6023)
                     /locus_tag="PKW60_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98761.1"
                     /translation="MDFAQGLCGRVMLGAHGIQIDREEQCTAVDVILKLGFDHVHLFL
                     DGFGPGFLPKVDSIGVVFVAALTVPGHGRIMEELVEPCADERIAAFDLVIEERKRQVG
                     VEGFDPEAEPAELDGERIEVYTVDTAFDHIAAQDGFGAQLEVVVLQTAGQLFVRKQFS
                     AILVINEFQDRLIPAVLNTIVDDQ"
     gene            complement(6453..6896)
                     /locus_tag="PKW60_04705"
     CDS             complement(6453..6896)
                     /locus_tag="PKW60_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98762.1"
                     /translation="MNLEDPFSILEPEDPFPRTVIQKQAAQRPCAVVAVEAGGHDEAK
                     AAAGAQQRMGLFKEHLVCVSFPSTLVLEDMFGIGIAGNFLALLFPAGMALPGRFEAAA
                     PPVPKIQIVPLSVIAASGKPVAKRVSSIGRPICHGLKECVVFQPG"
     gene            7369..7740
                     /locus_tag="PKW60_04710"
     CDS             7369..7740
                     /locus_tag="PKW60_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002784626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPJ98763.1"
                     /translation="MTKIASYRDLKVWQESMTLAEEVYQATAAFPKEELYGLTSQMRR
                     AAVSIPSNIAEGWGRGSTPDYVRFLRIARGSLKELETQATLSARLGLMTREPEQAIGV
                     RVESIGRMLVALERSLQRRTS"
BASE COUNT         1691 a         2437 c         2236 g         1474 t
ORIGIN      
        1 gccctggttc gggcagcccg ttggacagcc atcaccccat atatggaccc ggatgaccag
       61 ggaccgatag accggctacc cgtctgttcg ccattccacg gctccatcca ggttgaggat
      121 tatcagctcg tcccccttct gaaagccttg cgcatgccac gcgtaaattt gcttatagct
      181 gacgatgttg gcctgggaaa gaccattgaa gccgggctca ttttgagcga attgctcatc
      241 cgccggcgca tcaaccgcat actcatattg actcccgcct cgcttcggat tcagtggcgc
      301 gacgaactct ggtcaaaatt ctctctgccg ttcgacgtca ttgatcatga cttcactctc
      361 aatctcaagc gctcactggg ggttgatgcg aatccgtggc gttgttcctc acgcgccata
      421 gcttcttacc attacctgcg ccagcctgat atccgtgaac agttcgagag cgcttgccgg
      481 acatctgaag gctctccgca cttagcctgg gatctgctgg tcgttgacga ggtgcacaac
      541 ttgatgccgt caccgttcgg cgaagacagc ctgctttgcc agatgctgcg ccggatcgcc
      601 ccccaattcg aacatcgcct gtttctcaca gccactccgc ataatggcca cacccgcgca
      661 ttttcgggac tcctcgaact gcttgacccc gttcgtttca gccgcaccga tgagttgcgc
      721 cctgccgagc gtgagcgtat caaacagatc gtcattcgta gactgaaacg agaggtaaac
      781 gaacgcacaa gtccgccgcg cttctgcaca cgcagaccgc cggaagcgct tgtcctcaag
      841 ttttcgaaag aggaacaagg ccttattgat gcgttcgaag cattccgtga gaaggttcgc
      901 agccttattg ctgccagttc gagaaaacgc cgacttgcgg ggagatttgc catcgagatt
      961 ctcggcaagc gcatgctgag cggaccggtt accttcctcg aatcgtggcg ccggtgcaag
     1021 ataggcctgc aactcgaaga tcccgcaaat gacgacgacc tgcgagccgc cggagaagcc
     1081 gccaacgaag aaaccgccga cgaccgtgaa gccgaacagc gcggagcgac ggccgcggcc
     1141 gttatcggcg cctggatgca gcctctcgcc aaagatctgg aggcggagat tgccagaatt
     1201 gacgccgcgg cgaaacacat gggcatttct cttgataacg aggtcgtgtc ccagaatccg
     1261 gccggtgatg cccgtttcga ggtactccgt tggttcatcg accaacggct ccgacaagag
     1321 agcaactggc gtgacgacga acgattggtc cttttcaccg aatacaaaac tacgctcgac
     1381 tacctcttgc ggcgtctgcg cgagagcttc ccggggcagg aagatcgttt cctcagcctg
     1441 tttggcggga tggacgacat aaaccgagag gcgataaagg acgcgttcaa cgacccggac
     1501 cacagagtcc gcatcctgat cgccaccgat gcggcttctg aaggcctcaa cctccagagc
     1561 accgcgcgct acgtccttca tttcgattgt ccctggaacc cggcacgtct ggagcaacgc
     1621 aacggccgcc tggaccgcca cggtcaggcg cgtgatgtcc acgttttcca tttcgcaagc
     1681 gaacagaccg ccgacctgaa gttcctcgcc tacctcattc acaaggtcca tcaaatacgc
     1741 gaagacctcg gggccacagg cgagcttttt gatgaagcga cccacaagtg cctggttctc
     1801 ggtgaagagc ctgcagccgt gcagagaacc ctcgatatcc agattgaaca cacgaagggc
     1861 gccacccaga tggacgccga cgatacggtc agcgaagact ccagtcacgg cgaaagtggg
     1921 ctgtctgaca agttaaatgc gctggcggac gcgctggacc ttgaccccgt atccggacac
     1981 gccacgctcg aggcggccat ggccgcggac ggcggacgcc ctcaattgac cgtgccagac
     2041 gacacgctgc gtttttctct gatcaatccc catctcctgg gttggaaaga cacgattgac
     2101 gagaccattc gtaaacgcac cggccaaggg ctgctcggcc cggtcccaag gctcacgttc
     2161 agcgtcaagc ccttcatggt gccggtgggc gagcggaccg tgttccggcc tcgtgtcgac
     2221 accctgatgc ttcatctggc ccatcccctg atgcaacagg caacgggacg cctcacgcgc
     2281 caccggttcc ccggaccaaa cgccgtctcg cgatggacgg tcaagctggg agacgtgccc
     2341 gcggatgcgg atgcgctcat tctccttcat gtcgaggaac tggccgtgaa ccagctccgc
     2401 gagaccttcc atcactgggt acgaaccgtt gcgattccag tcgcggacgg tgagttgagg
     2461 aaaccactcg ctcccatgcc agccatcacc ctgcgcggcg cccaaccgtg ccgcgcgccg
     2521 gaattgcggg cgcaggccgc ttcactcctg agtgaccttg agccggagtt gcaggaattc
     2581 gttaaaaagc aacgccggga gttgactgcg aacctgcaga aacaactgga aatagaccgc
     2641 gaaaaggcgc ttgaagaaga ggaacgccgc ttccagagcc gtcagggcga ggtctccact
     2701 ttgattgccg agaatactct ggcgaagctg caacggcaga ttgaccggct caaacggatg
     2761 agtcagcagg gaatgctctt tgaacagcag gcctactgtg acgaaatcga ccggagcatc
     2821 gagctgaaac aggaggagtt gctgaggcgt aaacagcatt acgaagaggt ccgccagcaa
     2881 ctcgcccagg agcgtgaacg cattgtaagg cgcctgctgc caaaacgatt cacgctgcag
     2941 ggcgaagctc aggtctttcc tgtggcggtt gaggtccggt ttccgcgtga gcaaggaggt
     3001 gcgaagtgaa agccgattac ctccactggt ggaacaatct ccggcacggc ggccttcttc
     3061 tcgatcttca gcggctgagc aaactgctgt cctcgatgcc gtcagcgatg ccggaatggc
     3121 agcaagatcg ccttcgccgg gaagtcatcg cgtttcagaa tacccctgac gagaaacgca
     3181 gcgcgttcgt cgcctttgtt ctggcagacg tctgcggatt ttccggcgaa cacggtgaat
     3241 ggcatcgggg cagcaacatt tcatctgtct ggacgcgcca cggcctgacc ggcgaaagcc
     3301 tccgcccgaa ccatctctgg ctgagccggc atggctccgt cctgcccgtt ttcctggact
     3361 ccgagaagcg catcggtatc ggcaggggaa agcgcattac tgcccgttgc ctccagtggt
     3421 tacgacaggg cgatgaacaa ctcgctctgg tcaccaacgg ccatcaatgg cgcataatct
     3481 ttgccggcct cgactacgac gcctgggctg aatgggaact cgaacaatgg caggcagaag
     3541 gccggttttc ggacgaattc gatgcctttc gcgcgctgat atccccctcg ctgtggatgg
     3601 cgccagaaaa aggccagacc tgtcccctgt tgacggcgat caacgacagc cgcaaaggcc
     3661 agtccgatct gtccaaaatc ctgggcgaac gcgttcgaca ggccgctgaa ttgctcatag
     3721 acggccacgc gcccgcgctc aacctctgtc gcgaccaact cgatccgcaa gacatttacc
     3781 gggccgccgt gcgtgtcatc atgcgcatgg tcgttatcct cttcgcggag agccgcgaag
     3841 ggcttctgcc gcgggacaac ccggtcttta actccgccta cagccttcag gggctgcgcg
     3901 agctgcttga tcgcggcggc caatcccggc atgtggccaa ttgcagcgcc tggccgcgca
     3961 tcctggccct tttccgcctc atctatcagg gttccgatca cgaagccatg cccgtgccga
     4021 aatatggcgg cgaactcttt gcccccggct gcgcaaaagg ctcagacggt atgaaaaggg
     4081 cgatttatgt ttttgaaaat gcctgtttcg agcaggacgt catgaatgat gcgctggtgc
     4141 ggcaactgct cgacatgctc acacgaacga gagttacgat ccgccagggc cggtccagtt
     4201 cgtggatgat ggcgccggtc gacttctcca gtctcgacag cgagtacatc ggcattctct
     4261 acgaaggtct tctggatttt gaactccgct gcggcgtgga cggtcaaccc attgtgttcc
     4321 tcgccgtggg caaccagcct gcgttgcccc tggccacgct tgaagccatg aacgacaaag
     4381 cgatcaggaa cctctttgaa aaactgaagg acacgtcctc caaagacgac gacgaggaag
     4441 aggagatgga cgatggccag tggtcagtgg acgacgaaga aagtgaggat gaagacgtcg
     4501 aagaagaaga tggtgattct tggatggtag acggtgaaga atcggatggc tccgaaggcc
     4561 cctcttcacc ggacaccgac caccagccac cggccaccga agatgatccc cgctataccc
     4621 tgcgtgcccg ggccgaacaa tgggcccagc atgctgtaga agtcgccaag ctggtcaaga
     4681 aacctcgcgg caagctgacc cccgaaaagc ggatggcctg ggaacgcgaa cttgcttcca
     4741 aagcgcgcca actcattacc aaggtcgttc tccccggcga atggtacctc gtgcgctggg
     4801 gcggcacccg caaaggttcc ggcaccttct acacccggcc gcagctcgcc atccccaccg
     4861 tctaccgcac cctcgaaccg ctgtgctatg aggtgctcag tggccagggg cctgtggccg
     4921 gtgaagaaaa ggagatgaaa ggtgactgtc cagagtcacg aggacctgga agtgtggcag
     4981 aagggaatgg acctcgtgac gctcgtgtac aagctgacag cgtcatttcc gaaagcagag
     5041 atctacgcgc tgacgaatca acttcgacga gcagcggtat cagtgccttc gaacatcgcg
     5101 gagggccagg gacgcaggtc aacaaaggag tttctcaacc acctctcgat ggcgaaggga
     5161 tccctcatgg aagtggagac ccagacagcc atcgcgttga gactgaaata tctgaaacag
     5221 gaaaagcaag agcaagtgaa ggcgctgaca ctggtcacgg ggcgcctgct caacggttta
     5281 attcgagctc tgcgacacaa gctgtagcct cttcttcctc ctctccggcc actgaccacc
     5341 ggcaaccggc cacccgtctt ccaaagctcc ctgaacagat cctggcattg aaaatctgcg
     5401 accccgcatg tggctccggc tcattcccgc tggcagcact gcgttatctg acaaacgccc
     5461 tctacgactc actgatcgtc cacaatcgta ttcaagaccg cggggatcag gcggtcctgg
     5521 aactcattta taacgaggat ggcagagaac tgcttgcgga cgaaaagttg ccctgccgtc
     5581 tggaggacga cgacttcgag ctgcgcacca aagccgtcct gcgccgctat gtggtcgaac
     5641 gctgtatcta cggtgtagac ctcgatccgc tcgccgtcga gctctgccgg ctcagcctct
     5701 ggatcgaaac cctcgacccc aacctgccgc ttacgttcct caatcacaag atcaaatgcg
     5761 gcaattcgct cgtcggcgca tggttcgacc agttcctcca ttatccggcc atggcctggg
     5821 accgtgaggg cggcgacaaa aaccactcca atggagtcca ctttcggaag gaagcctgga
     5881 ccaaagccat caagaaaaag atgaacgtgg tcaaagccga gcttaagaat tacatcgacg
     5941 gcggtacact gttcttccct gtcaatctgg ataccgtgcg caccgagcat gacgcggccg
     6001 cagaggcctt gcgcgaaatc cacgcctatg gaatcaccca ggttcaggaa cgggcagaac
     6061 gctacgaggc gctgatttca gacccggact tcatccgcct caaagaggcg tttgacctct
     6121 ggtgcgcgct ctggttctgg ccgcccgatc atctcgacct ggcccccttg cccctggcct
     6181 ttgccgcggg ggagctatcg gatgaggccc gagccatagc ccgccgtgtt gccgcggaac
     6241 gccgcttctt ccactgggaa ctcgagtttc ccgatgtgtt ccacgcggcc gggcagggct
     6301 ttgatgccat cctcggcaat ccgccctggg agaccctaca accagtttcg aaggagttct
     6361 tctctgccat cgatcccatg taccgcagtt acggtaagca ggaagctctt ggaaggcaga
     6421 aagagctttt cctagaggat gaaagtgccg agctatcctg gctggaatac aacgcattct
     6481 ttaaggccat ggcaaattgg tctgcctatg ctggacaccc ttttggcgac aggcttaccc
     6541 gaggcagcaa tgaccgagag aggcacgatc tgaatcttgg ggaccggggg cgcagcagct
     6601 tcgaaacgtc cgggcaacgc catgcccgct ggaaacaaaa gcgcgaggaa attaccggct
     6661 ataccgatcc cgaacatgtc ttccagcacc aaggtactgg gaaagcttac acatacaaga
     6721 tgttccttga acaggcccat gcgttgctgc gctccggcgg ccgccttggc ctcatcgtgc
     6781 cctccggcct ctacagcgac cacggcacac gggcgctgcg ccgcttgttt ctggatgact
     6841 gttcgtggga atggatcttc gggttcgaga atcgagaagg gatcttcgag attcaccgtt
     6901 cgttcaaatt caaccccgtc atcgtcgcga aaggcgggag cacccaggcc atccgcaccg
     6961 ctttcatgcg gcgcgacctc gcggactggg aacacggcga agacttcgcc acactgtatc
     7021 cccgcgagcg catcgtccag ttcagcccca acagcctcgc catcctcgaa atccagtctc
     7081 cccgcgacct cgaagttctc acgaaaatct acgccaacag cgtcctcctt ggcgaccagg
     7141 gacctgacgg ctggggtatc aagtacactc agggcgactt caacatgacc agtgattcta
     7201 agctgttccc gccgcgcacg aaatgggaag aatggggcta ccggcccgat gagtacagcc
     7261 gctggatcaa ggggccgtgg aaacccgtgt ccgagctgtg gaaagaactg ggagtggcga
     7321 gtggcgagtc gccagtgggg aatgggcagg cgaaaggaga ttcgggtgat gacaaagatc
     7381 gcgtcatacc gggatctgaa ggtttggcag gagtcgatga ctttggcgga ggaggtttat
     7441 caggcaacgg cggcattccc gaaggaggag ctgtacgggc tgacgagcca gatgcggcgg
     7501 gcggcagtgt ccattccctc caacatcgcg gaggggtggg ggcgaggctc gacgccggac
     7561 tacgttcgct ttctccggat cgcgcggggc agtctcaagg aactggagac ccaggcgact
     7621 ctgtccgcgc ggctggggtt gatgaccagg gaaccggagc aggccatcgg ggtgcgggtg
     7681 gagagcattg gccggatgct ggttgccctg gaacgcagcc tccaacgcag gaccagctag
     7741 cgacgccccc actccccact ggcccctccc cacccccccc ccgccccccc cccccccccc
     7801 cccgcccccc cccccccccc cccccccccc cccccccc
//
LOCUS       DAOPBQ010000099         7796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8503_length_7796_cov_4.401111, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000099 DAOPBQ010000000
VERSION     DAOPBQ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8503_length_7796_cov_4.401111"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="PKW60_04715"
     CDS             complement(<1..1515)
                     /locus_tag="PKW60_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98764.1"
                     /translation="MIVTTLTCTMLFAALLGGTEVSPGTEMILRLPPPTPQSAPPEAG
                     LAYGPVEGFVPPRTIVFSVGPDELEADAEAWVAHGVTAFFLDFIAREWSSDIWARDGK
                     PWTIGESDETFQAAKRANARCAALGAETFLKVSFDHYFEWFNDLMWQQAYHNFRQFAI
                     FAREAGCTGLALDIEYVGEQYRFDWEGYTYDGYTKADLANMIRERMTRVVAILYDEFP
                     DMIFLTFPEQGLGLGSIIHTAWIEEAARRNAPGGVHYCTEHTYRDTNIREVFAYTAAI
                     NDLFHRVLTPQAWRYWQDHGSIAVGLWPFGFDYQNVFEPGLTVEQYRQGYAASLMVSQ
                     RYNWIYSHNCRDLLINRNADKYAGAEPLQAYLDVVQKKEMVITAPYPAVAADLRAKTL
                     RDYAPELERTHFVGFIGPAEAPQLQVAPAGLWSHDELERCWELALQYPQGRELNLRQA
                     FGTVTDWMVIGPFPNGEGFAGHRAVYPPEERLDFGAEYDGVEGAVRWRPVHSEGN"
     gene            complement(1572..3068)
                     /locus_tag="PKW60_04720"
     CDS             complement(1572..3068)
                     /locus_tag="PKW60_04720"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPJ98765.1"
                     /translation="MTRRFACLILMIALGSAAATAAEPLGIGNMESLTRILPGRSMRS
                     STCDPDWRNGNADARPIAPGETLVLADLEGPGVITHLWNTIAAEERGYSRLLVLRMYW
                     DGEEHPSVEAPIGDFFGIGHGLDRPYVSLPFKISSEGRARNCYWPMPFRKSARITVTN
                     EGRKRINAFYSYVDWRQLPELPEDTVYFHAMYRQEYPAVMGQNYLITEITGRGHYVGT
                     VLNCEQLSQGWFGEGDDFFFIDGEEEPSLRGTGTEDYFCDAWGFREQDGPFYGTPLFE
                     GYDTMNRTSAYRWHITDPVLFEQSLRVEIEHKGSAHDDTGRIISGFTERPDNFSSVAY
                     WYQTEPHKPFPALAPAYDRLSIDYANMIEAEQWLDSAITDGVAPGLQEGGAWSAGGQV
                     FWTCTEPDKSLTLFFDVTETGTYDLLFVLTFSYDYGIFEFQLDGQPLGRVLDLYHATV
                     MVDERYMGTRELSAGPHTITVKNMGKSAESKGYYFGFDGIILNRKKDQ"
     gene            complement(3256..5202)
                     /locus_tag="PKW60_04725"
     CDS             complement(3256..5202)
                     /locus_tag="PKW60_04725"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPJ98766.1"
                     /translation="MIDPALILLVIGFLVLVSILASRVSDRAGVPTLIVFLCIGMLAG
                     SDGPGGIVFNNTQAANLIGTIALAFILFAGGLDTHWGAVRPVLGRGILLATAGVVITA
                     GLVGLFLWKVLGFEPLTGLLVGSIVSSTDAAAVFAIMRRRGVSLKGHLKPLLELESGS
                     NDPMAIFLTAGVTRLLMEPDFEWTSLLPALAWNMLGGMALGLLAGKLAALLFNRIRLG
                     YEGLYPVLSISIVLLTFGMTQFIKCNGYLAVYLCGIMLNASRFNYHLYVAKFHDGLAW
                     LMQIGLFLVLGLLVNPSQLPRIALTAAPIVLFLMAVARPVAVALGLVASSFSWRERAL
                     AAWTGLRGAVPIVLATFPLMAGFSQSDMVFNIVFFIVLTSVLTQGTLLMPVARWLKVD
                     KPLPPLPRYTLQIQREGQTQGETREVEILPSMAAVGRTVADLDIPPGVLILLIGRGDG
                     FVVPRGQTRIEPYDTLLMMGDPNALRAAGDAILSPRVKVRQREPQDDPLEMLPENTDE
                     KFLSKHVVVAGFGRLGRRIYDKLKARNVPLVVLDQDRELIQQLRQDHCAAVAGDASTA
                     MALAQAHVARAAMLVVATPDAMKATELAALARQLNPNIEIVICAKTEMEAKVLEHEKA
                     GTVFLSEEALAGSVVQFVFKRLRA"
     gene            complement(5388..6923)
                     /locus_tag="PKW60_04730"
     CDS             complement(5388..6923)
                     /locus_tag="PKW60_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPJ98767.1"
                     /translation="MLARVRSCGVLGIDGYPLEVEVDNHPGPRRFTMVGLPDAAVKES
                     QERVQSALRSAGFRNLRGVVVVNLAPADVRKEGSALDLPIALGMAAAGEQLPASRFLE
                     YAIVGELALDGSVRPVAGALCMAMAARDQGLRGILLPRANAEEAGVVPEIDVIPVATL
                     SEAAEFVTGRSDIVPFRTNLQGALSQARAHSLDLTDVKGQAHVKRALTVAAAGSHNLL
                     MIGPPGTGKTMLASRLPGILPEMTFEEALETTRVYSVAGHLSDKGNLVCRRPFRAPHH
                     TATTVSISGGGHGLQPMPGEVSLAHNGVLFLDELPEFNRAALEVLRQPLEEGLVHIRR
                     AAYAVTFPSRFMLVVAMNPCPCGCRTDPRRECRCSVGEVQRYMSRLSGPLLDRIDLHV
                     DVPALSFEEMADDRPSGTTTAEVRAQVQAARERQHARHNGRFRGNAHLDATATRKHCV
                     ISTGGKALLEAALERMGLSARAYDKILRVARTLADLDDQEQIREQHLAEAIQYRSLDL
                     ELR"
     gene            7149..7757
                     /gene="cysC"
                     /locus_tag="PKW60_04735"
     CDS             7149..7757
                     /gene="cysC"
                     /locus_tag="PKW60_04735"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HPJ98768.1"
                     /translation="MTDIKSKNITWHESNITKEDREKLNGHKAVVLWFTGLSGSGKST
                     LAHAVEQALYDKGCRTIVLDGDNIRHGLNKNLGFSPEDREENIRRIGEVAKLFTQAGV
                     IALTAFISPYRADRDKVRALLNDGEFIEVYLQCAIEVCESRDVKGLYKKARAGEIKEF
                     TGISAPYEEPLQAELVIDTAAITLEDSARAVLAFLRDRGIVA"
BASE COUNT         1532 a         2488 c         2348 g         1428 t
ORIGIN      
        1 attcccttca gagtgcacgg gccgccatcg aaccgcgcct tccacgccat cgtattccgc
       61 gccgaagtcg agtctttcct caggcgggta caccgcgcga tgccccgcga agccttcccc
      121 attgggaaac ggcccgatga ccatccaatc ggtgaccgtg ccgaacgcct ggcgcaaatt
      181 gagttcccgg ccctgcggat attgcagggc aagctcccag cagcgttcga gctcgtcgtg
      241 gctccacaga cccgccggcg ccacttgaag ttgaggcgcc tcggcgggcc ctataaaccc
      301 gacaaagtgc gtgcgctcga gttcgggcgc gtaatcgcgg agtgttttgg cccgcaagtc
      361 cgccgccacg gcgggatacg gcgccgtgat gaccatctct ttcttctgaa ccacgtcaag
      421 ataggcctgc aggggttctg cgccggcgta cttgtcggcg ttccggttga tgaggagatc
      481 gcggcagttg tggctgtaga tccagttgta gcgctgcgag accatgagcg acgccgcata
      541 tccctgtcgg tattgctcga cggtgaggcc gggttcaaac acgttttgat aatcaaaacc
      601 gaacggccat aagccaacgg cgatagagcc gtgatcctgc caataccgcc aggcctgcgg
      661 cgtcagcacc cggtggaaga ggtcgttaat cgccgcggtg tacgcgaaca cttcgcggat
      721 gttggtgtcg cggtacgtat gttcagtgca gtaatgcacg ccgccggggg cgttgcggcg
      781 cgcggcctct tcgatccagg cggtgtggat gatggagccc agccccagcc cctgttcggg
      841 aaacgtgagg aagatcatgt ctggaaattc gtcgtagagg atagcgacaa cccgggtcat
      901 gcgctcgcgg atcatgtttg cgaggtcggc cttggtgtat ccatcatacg tgtagccttc
      961 ccagtcaaag cggtattgtt cgcccacgta ctcgatgtcc agggccaggc ccgtgcaccc
     1021 ggcttctcgc gcaaagatcg cgaactgccg gaaattatga tacgcctgct gccacatcaa
     1081 gtcgttgaac cactcgaagt aatggtcaaa cgacactttc agaaacgtct ccgcaccgag
     1141 tgctgcgcat cgtgcgttgg cgcgctttgc cgcctgaaac gtctcgtcgg attcgccgat
     1201 ggtccagggc ttgccgtccc gcgcccagat gtcgctcgac cattcgcgcg cgatgaaatc
     1261 gaggaaaaaa gccgtcaccc cgtgcgccac ccaggcctcc gcatcggcct ccagttcgtc
     1321 aggacccacg gaaaacacga tggttcgagg gggaacgaag ccttccacag gtccgtacgc
     1381 aagacccgct tcgggtggag ccgactgagg cgtgggcggt ggcaggcgga ggatcatttc
     1441 agtacccggc gatacctcgg ttccgcccag tagcgcggcg aagagcatcg tgcaggtcaa
     1501 cgtcgtgacg atcatcttag gttcctttct gtgacgcagg gcggctgcga acctggacct
     1561 gccggggccg cttactggtc tttcttccgg ttgaggatta tcccgtcgaa accgaagtaa
     1621 tatcctttgg attctgccga tttgcccata tttttcacgg tgatcgtgtg cgggcccgca
     1681 ctcagctcac gggtgcccat gtaccgttca tcgaccatga cggtcgcatg atagagatcc
     1741 aaaacccggc ccagcggctg gccgtcgagc tggaattcaa agatgccgta atcgtacgag
     1801 aaggtgagca caaacaagag atcatacgtg ccggtttccg tcacatcgaa gaacagcgta
     1861 agcgatttgt ccggttcggt gcaggtccag aacacctggc ccccggcgct ccacgcaccg
     1921 ccttcctgaa gtcctggtgc gacgccatcc gtgatcgccg aatccagcca ttgctccgct
     1981 tcgatcatgt tggcgtaatc aatgctcagc cggtcgtatg cgggcgccag cgcggggaat
     2041 ggcttgtgcg gttcggtctg ataccagtac gccaccgacg agaaattgtc ggggcgttcc
     2101 gtaaacccgg aaatgatccg gcccgtatcg tcatgggccg aacctttatg ctcaatctcg
     2161 acccgcagcg actgttcaaa cagcaccggg tccgtgatgt gccaccggta cgcgctcgtg
     2221 cgattcatcg tatcgtatcc ttcgaacagc ggcgtcccgt aaaacgggcc gtcctgctcg
     2281 cggaatcccc aggcgtcgca gaaatagtcc tcggtgcccg taccgcgcag actcggttcc
     2341 tcctcaccgt cgatgaagaa gaagtcgtct ccttctccga accagccctg cgacagctgt
     2401 tcgcagttga gcacggtgcc tacataatgg ccgcgcccgg tgatctcggt gatcaggtaa
     2461 ttctgcccca tgaccgcggg gtactcctgc cggtacatcg cgtggaaata cactgtgtcc
     2521 tcaggcagtt cggggagttg ccgccagtcc acgtacgaat aaaaggcgtt gatccgcttc
     2581 cggccctcgt tggtgacggt gatgcgcgcg gatttgcgga agggcatggg ccagtaacag
     2641 tttcgcgcgc ggccttccga cgagatcttg aacggcagcg acacatacgg gcggtccagg
     2701 ccgtggccga tcccgaagaa atcgcctatc ggcgcctcga ccgacggatg ttcttcgcca
     2761 tcccagtaca tccgcagcac caggaggcgc gaataccccc gctcctcagc cgcgatggtg
     2821 ttccacagat gggttatcac gcctggccct tccaggtctg ccagcaccag cgtttcgccc
     2881 ggcgcgatgg ggcgcgcatc ggcattgccg ttgcgccagt cggggtcgca ggtcgacgat
     2941 cgcatggacc gtcccgggag aatccgggtc agcgattcca tattcccaat ccccaacggt
     3001 tcggcggccg tggccgccgc gcttccgagg gcgatcatca gaatcaggca ggcaaaacga
     3061 cgtgtcatgg ctgtgtcctc cgcgtgagac cagtctggtg caccaaacga aaaacctagg
     3121 atacaccgga acgcatccgg gaagaaatgc cggtgcgcgg gcgatttctg gccgcttcag
     3181 agatttccgt gttcaagcgg ggacatgttg ccgcgccggg ggcagcatat gcgatacacc
     3241 gccagatgca cgagctcagg cgcgcaatcg cttgaataca aactgaacga cgctgcccgc
     3301 cagggcttct tcgctcagaa agaccgtgcc ggccttctcg tgttcaagca cttttgcttc
     3361 catttcggtt ttggcacaaa tcacgatctc gatattcgga ttcagctgcc tggccagcgc
     3421 cgccagctcg gtcgccttca tggcatcggg agtggctacg accagcatgg cggcccgggc
     3481 cacgtgggct tgcgccagcg ccatggcggt cgatgcatcg cccgccaccg ccgcacagtg
     3541 gtcctggcgg agctgttgaa tgagttcgcg gtcctgatca agcactacca gcggcacatt
     3601 gcgcgccttc aacttgtcat agatgcgccg cccgagacga ccgaacccgg ccaccacgac
     3661 gtgttttgac aggaattttt catcggtgtt ctcgggcagc atctcgagcg gatcatcctg
     3721 tggttcccgt tgccggacct tgacccgcgg ggagagaatc gcgtcgcctg ctgcgcgtaa
     3781 tgcattcggg tcgcccatca tcaggagcgt gtcgtagggc tcgatgcggg tctgcccgcg
     3841 gggcacaacg aagccgtcgc cgcgcccgat gagcaggatc agaacgccgg gcgggatgtc
     3901 gaggtcggct acggtccgcc cgacggccgc catggaggga aggatctcga cctcgcgcgt
     3961 ttcgccctgg gtctgacctt cccgttggat ctggagcgtg taccggggca gtggcggcaa
     4021 gggcttgtcc accttgagcc agcgggccac cggcatcagc agcgtgcctt gggtcagcac
     4081 cgaggtcagg acaatgaaaa acacgatgtt gaacaccatg tccgattgcg aaaacccggc
     4141 catcaacgga aacgtggcca ggacgatcgg cacggctccg cgaaggccgg tccaggcggc
     4201 cagggcccgt tcccgccacg aaaaactgct cgctaccagg ccgagcgcca ccgcaaccgg
     4261 ccgcgctacg gccatcaaaa acaagacaat cggggccgcg gtcagggcga tccggggcag
     4321 ctgcgacgga ttgaccagca ggccgagaac caggaagagg ccgatctgca tgagccaggc
     4381 gagaccgtcg tgaaattttg ccacatagag gtggtagttg aagcgcgacg cattcaacat
     4441 gatgccgcac aggtacacgg caagatatcc gttgcacttg atgaactgcg tcatcccgaa
     4501 cgtcaacagc acaatgctga tgctcaacac agggtataac ccttcgtaac cgaggcggat
     4561 ccggttgaac agcagcgccg ccagttttcc cgcgagcaaa cccagcgcca tgccgcccag
     4621 catgttccag gcaagcgccg ggaggagcga cgtccattca aaatccggtt ccatcaggag
     4681 ccgcgtgacg cctgccgtca gaaagatggc catcggatcg ttgcttcccg actcgagttc
     4741 gagcagcggc ttcaaatgac ccttcaggct cacgccccgg cggcgcatga tcgcgaacac
     4801 cgccgccgca tctgtcgacg agacgatcga acccaccagc agcccggtca ggggttcaaa
     4861 accgaggact ttccataaga aaagcccgac caggcccgcg gtgatcacga ccccggcggt
     4921 tgccagcaga atgccgcgcc ccagcacggg acgcaccgcg ccccagtggg tgtccagacc
     4981 tccggcaaac aggatgaacg ccagcgcaat ggtgccgatc agattggccg cctgcgtgtt
     5041 gttgaacacg atcccgcctg gaccgtcaga tcccgcaagc atcccgatgc acagaaacac
     5101 gatcagcgtg ggcacgccgg cccgatccga cacccggctc gccaggatgc tgaccagtac
     5161 caaaaacccg atgacgagaa gaattaatgc gggatcaatc atgaacaagc catcttcagc
     5221 tgagtttcgt ggcccacctt ccggttcgtg gaggcgaacc gtgcgcttcg cggcaaaaca
     5281 ttcccatcgg ttatgatagc agacaccggg ccaaagcgtc acgattgcag tgcagaaggc
     5341 gttgaaaggc gccgttttcg gggcgggggc cgggtggccg cacgccctta ccgcaactcc
     5401 aggtccagac tccggtactg gatggcttca gccaggtgct gctcgcggat ttgctcctgg
     5461 tcgtcgagat cggccagtgt gcgggcaacg cgcaggatct tgtcgtaggc tcgggcgctc
     5521 aggcccatcc gttccagggc ggcctccagc agtgctttcc cgcccgtgct gatcacgcaa
     5581 tgtttgcgcg tggcggtggc gtccagatgg gcattgcccc ggaagcgtcc attgtgccgg
     5641 gcatgctgac gttcgcgtgc cgcctgcacc tgggcgcgca cttcggctgt ggtcgttccg
     5701 ctgggccggt catcggccat ctcttcgaac gacagggcag gcacatccac atgcagatcg
     5761 atacgatcca gcagcggccc cgataaacgg ctcatgtagc gctgcacctc gcccacgctg
     5821 caccggcatt cccggcgggg atcggtccgg catccgcacg ggcagggatt catcgcgacc
     5881 accagcatga agcggctggg gaacgtcacg gcatacgcgg cacggcgaat gtgtaccagc
     5941 ccttcttcga gcggctgccg cagcacttcg agggcggccc ggttgaattc gggcaattca
     6001 tcgagaaaca gcacgccgtt gtgcgccagg ctcacttccc cgggcatcgg ctgcaggccg
     6061 tgcccgccgc cgctgatcga caccgtggtc gcggtatggt gcggcgcccg gaaaggacgc
     6121 cggcagacca ggtttccctt gtcgctcaag tggcccgcca cgctgtagac ccgcgtggtc
     6181 tccagcgcct cttcaaatgt catttcggga agaatccccg gcaggcgcga cgcgagcatc
     6241 gtcttgccgg tgccgggcgg gccaatcatg agcaggttgt gcgaacctgc cgcggccacc
     6301 gtcaaggcgc gttttacatg cgcctggccc ttcacgtccg taagatccag gctgtgcgcc
     6361 cgggcttgcg aaagcgcccc ctgtaaattg gttcgaaacg gcacgatgtc gctgcgcccg
     6421 gtaacaaatt ccgccgcctc gctcagcgtc gcgacgggga tcacgtcgat ttcaggtacc
     6481 acccccgctt cttcggcgtt ggcccggggc agaaggatgc cgcggagccc ctggtcccgc
     6541 gcggccatcg ccatgcataa cgcccccgct acgggacgaa ccgacccgtc aagcgccaac
     6601 tcgcccacga tcgcgtattc gagaaaccgc gacgcgggga gctgctctcc tgccgcggcc
     6661 attcccaggg cgatggggag gtcgagcgcg ctgccttcct tccgcacgtc cgccggagcc
     6721 agattgacca ccaccacgcc ccgcaggttc cggaacccgg cgctccgcaa cgccgactgc
     6781 acgcgctcct ggctttcctt caccgccgcg tcgggaagcc ccaccatcgt gaagcgccgc
     6841 gggccggggt ggttgtccac ctccacttcc agcggatacc catcgatccc cagcacgccg
     6901 cacgatcgca ctcgcgccaa cataaaacga catggcctct gttgcgttaa tcacattaac
     6961 aaaacagtgc atcataatac ggtgattgcc cccgcgaatc aaccggccgg gatgagattt
     7021 ccggccgcac gtgatagact ggcggctctt atgtatctgg acgcgcgggt atctgcgcgt
     7081 gcgccgggat tccacgccgt ccggcgccgc ctgggtcccc ggaaggccgc gatttatgga
     7141 gtgaatcaat gacggacatc aagagcaaga acatcacctg gcatgaatcc aatatcacga
     7201 aagaagaccg cgaaaaactg aacggacaca aggccgttgt cctgtggttc acggggcttt
     7261 cgggttcggg caagtccacg ctcgcacatg cggtggagca ggccctgtac gacaagggct
     7321 gccgcaccat cgtgctggac ggcgacaaca tccgccacgg gttgaacaaa aacctcggtt
     7381 tctcgccaga agatcgcgaa gaaaacatcc gccgcatcgg cgaagtggcc aaactcttca
     7441 ctcaggccgg agtgattgcc ctcacggcat tcatctcccc gtaccgggcc gaccgcgata
     7501 aggtgcgcgc actcctcaat gatggcgaat tcatagaggt ctatctgcaa tgcgccatcg
     7561 aggtctgcga atcacgggat gtcaaagggc tgtataaaaa ggcccgcgcc ggggaaatta
     7621 aggaatttac gggcatttcc gccccgtatg aagagccgtt gcaggccgaa ctcgtgatcg
     7681 acaccgcggc cataaccctt gaagattcgg cccgggcggt tctggcattt ctccgcgatc
     7741 gaggaattgt cgcctgaaag gccggatttt cggggacatg ccgcctgtgc ctgctc
//
LOCUS       DAOPBQ010000100         7796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8504_length_7796_cov_3.973776, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000100 DAOPBQ010000000
VERSION     DAOPBQ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8504_length_7796_cov_3.973776"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PKW60_04740"
     CDS             complement(<1..757)
                     /locus_tag="PKW60_04740"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HPJ98769.1"
                     /translation="MTGLLLVILTAHVTTAAAPSAPELDEARRWAAARFEGKAEEVPL
                     HPGIRVLANNDPVQMNARDGHPLRLVDVEYTRGLYCHANSRVVVQIAQPGQRFDAVVG
                     VDSNEQTSGGRGSVVFGVELGGTQVWHSEIIREGMPGVPVSIDLGGATEFVLTVDDAG
                     DGIACDQSDWADARVVLDDGTEVWLGDLAQLGPQRSPYAVEPFFSFTCGGKRSSDFLD
                     AWMLERRTERIAGRVTSHTLTYTDPETGLVVRCQ"
     gene            complement(900..1364)
                     /locus_tag="PKW60_04745"
     CDS             complement(900..1364)
                     /locus_tag="PKW60_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPJ98770.1"
                     /translation="MVLTELLKANTILADVGQPDKWTLIERLVDLLIDSGKAVDRAAL
                     LNAILSREKLGSTGLGNGIAIPHARTDGVREVAVALGISPSGMDFDAADGKPCHLIFL
                     IVSPPEASIRYLEALAAVASLGEEPGLVSQLKSAKTANEVLALFRLYREQSE"
     gene            complement(1537..2028)
                     /locus_tag="PKW60_04750"
     CDS             complement(1537..2028)
                     /locus_tag="PKW60_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPJ98771.1"
                     /translation="MPPLPDFGKILVYIETNEHGLQAIRYAVAFARQFGGELHAIAVI
                     NERLLEELTRAHVFLKEEEIALKSDLEDDGRRYLALMERFAQEKELEVTTELLHGVVH
                     RQVLDKAREISANLIILGEIEESLSRKDTTFNESERIICGARCPVLVVRGRRVIQDFF
                     DSA"
     gene            complement(2032..2661)
                     /locus_tag="PKW60_04755"
     CDS             complement(2032..2661)
                     /locus_tag="PKW60_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPJ98772.1"
                     /translation="MAGKHIAPTRTNLLRLKGELAFARSGYELLEQKREILILELKRF
                     TARAIEAEQKTSQAMATAFEALQEAQFVTGVSGVVSASRAVNIESSLLLRERRVMGVP
                     LPAVELQMSGNPPYYGPSGTSVWTDEAVRRFKEALASLAALAECRISVLRLAKEIQKT
                     IRRVNALERIFIPDYEDNLHHVEAALEEADREAFFVLKLVKDRLEAEEE"
     gene            complement(2663..4078)
                     /locus_tag="PKW60_04760"
     CDS             complement(2663..4078)
                     /locus_tag="PKW60_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPJ98773.1"
                     /translation="MNLQASTAEISQRQLLDGREYVGLDEVSGPIVGVSGIHNIGYNE
                     LAEIVDRQGNTRLGMTLEVSEGAAVIQVFEGTSGLKLGDTRVRFRGRPLTLAVSEEML
                     GRAFDGLGRPFDGGPELIGEERDVNGLPVNPTARAYPRKFIQTGISAIDGMNTLVRGQ
                     KLPVFSGSGLPHRQLVAQITRQARIIGEDTPFAVVFAGMGIKHDEARFYLNSFEESGA
                     MERVVVFLSLADAPSVERLMTPRSALTAAEYLAFDLGMHVLVILTDMTNYCEALREIS
                     TQRQEVPSRKGYPGYLYSDLASIYERAGMLRDKPGSITQIPVVTMPNDDISHPIPDLS
                     GFITEGQIVLERELDQRSIYPPIAGLPSLSRLMKDGIGDGMTRADHANLASQLFASYA
                     HVKDIRSLASVIGEEELTDLDRKYMAFGETFERQFLSQGLEEDRSIEQTLDLGWKILQ
                     SLPSEELHRLTREEIGKYYGR"
     gene            complement(4053..5822)
                     /locus_tag="PKW60_04765"
     CDS             complement(4053..5822)
                     /locus_tag="PKW60_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPJ98774.1"
                     /translation="MAERIIGTVRRVVGPVVEAGHVSGVEMLELVYVGEERLVGEIVR
                     IRGEAAWIQVYEDTTGLRPGASIIGQGEPLSVELGPGLLGSIYDGVQRPLDVLRAQMG
                     DFVSRGVHAPALSREKRWHFTPCATPPCPISGGSVLGVVQETEVIEHRILVPPWVEGT
                     LTHLAPEGEYSIEDAVATVKTPDGDVELCLFHKWPVRKGRPYAKRLACAGPLITGQRV
                     LDTLFPVARGGVVVVPGGFGTGKTMVQHAIAKWCDADLTVYIGCGERGNEMTGVLTEF
                     PELIDPRTGKSLMERTLLIANTSNMPVAAREASIYTGITIAEYYRDMGYNVAIMADST
                     SRWAEALRELSGRMEEMPADEGFPAYLPTRLAEFYERAGLVTALSGREGSITTIGAVS
                     PPGGDFSEPVTQHTKRFVRAFWALDKQLANARHFPAISWLDSYSEYIDDVAPWWDEAT
                     NGRWRELRAAIMAILQKEARLEHVVKLVGADVLPDSQRLILEAANMFKNAFLQQNAFD
                     KIDAFCVAGKQYRMLNIIVTFHDLAAQAIQKGVTLASLKRMDAVGDIMQMKYECPNDE
                     LDRLDALLKELEESIHELTSIYG"
     gene            complement(5815..6465)
                     /locus_tag="PKW60_04770"
     CDS             complement(5815..6465)
                     /locus_tag="PKW60_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HPJ98775.1"
                     /translation="MSDEALESGGLQIVESILADADAQASRIIESAESAAADARKQAQ
                     MEGERGRDEVLEATRERCAKLRTQELATARIEARRLLVAARESVAERVLAQIEKKLEA
                     VRRSPEMCAKSLRNLAAEAAAAIALPEVVVRIYKDDAAFLSDAFPGEVAADPRAAAAG
                     VSRVILQLDSHDSGGGCIAQSTDGRIVYDNTYKTRMQRKRRELRAMIVEDVINSHG"
     gene            complement(6462..6767)
                     /locus_tag="PKW60_04775"
     CDS             complement(6462..6767)
                     /locus_tag="PKW60_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPJ98776.1"
                     /translation="MIPFVIGDDQTVLGFRLVGVEGRVASGRDQMLAALNEALGRREI
                     GLIVVSERLAVEIRDEIDARLSDAGFPLILEVPDASGPLPDRPGLAEIVRKAVGVNL"
     gene            complement(6769..7125)
                     /locus_tag="PKW60_04780"
     CDS             complement(6769..7125)
                     /locus_tag="PKW60_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HPJ98777.1"
                     /translation="MYKGIQYSKRAVRALLLASVVMLAALLWCSGAWAQPETAQAAPS
                     PDSGNAGWAYLAAALSVGLGCVGAGIAVAYVGSAAVGAIAEKPQLAARTLIFVGLAEG
                     IAIYGLIIGIMVLGKV"
     gene            complement(7138..>7796)
                     /locus_tag="PKW60_04785"
     CDS             complement(7138..>7796)
                     /locus_tag="PKW60_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPJ98778.1"
                     /translation="ELFAFAIGFGVLFVSLGIVLNILNAIRARAFWNALFDGAGPLAG
                     IIYWSGIGLVVKSFLNSSGMPHSAWLVVPMAIALLGFLMKGPLLKWSGKQERAFPEGV
                     ITYVMESAVELMEVVMGYLANTVSFIRVAAFGLAHAGLFVAVFSLADIVHGAPGGRFL
                     SLAVIVAGNVLIIVLEGVVVTIQALRLEYYEFFGKFFNRVGQKYAPVGAVPPPSDATN
                     "
BASE COUNT         1533 a         2492 c         2237 g         1534 t
ORIGIN      
        1 cttggcaccg gaccaccagc ccggtctcgg ggtcggtata ggtaagcgtg tggcttgtca
       61 cgcgtccggc gattcgctct gtccggcgct ccagcatcca cgcgtccaga aagtcgctgg
      121 agcgttttcc gccgcaggtg aaggaaaaga agggttcgac agcatacggc gagcgttgcg
      181 gccccagctg cgccaggtcg cccagccaaa cttccgttcc atcgtcaagc actacccgcg
      241 cgtcggccca atcggactga tcgcatgcga ttccgtcgcc cgcatcgtct acagtaagca
      301 cgaattccgt ggccccgccc aggtcgatcg ataccggcac gcccggcatt ccctcgcgga
      361 tgatttctga gtgccaaacc tgagtcccgc cgagctccac gccgaacacc acgctccccc
      421 gcccgccgct ggtctgttcg ttcgagtcaa cccccacgac cgcgtcaaag cgctgccccg
      481 gctgcgcgat ttgcaccaca acgcggctgt tggcgtggca atagagaccg cgggtgtatt
      541 ccacatccac cagccgcagc gggtgcccgt cacgggcgtt catctgaacc ggatcgttgt
      601 tcgccaacac ccggattcct gggtgcagcg ggacttcttc cgccttccct tcgaaccgcg
      661 ctgctgccca ccggcgcgcc tcatccaatt ccggagcgct gggtgcagct gctgtagtca
      721 cgtgagccgt gagtatgacc aagagcaatc ctgtcatggc accctcctgg actgcgcgcg
      781 ccgaaatatt ttccgtcagc ccaatacact aagcacggta gaactcgaac atcgacggcg
      841 tcaggcaaaa gcgcatcaaa aggaacccag gcgccctgtt ttacgcccta gtgcgcctgc
      901 tattcactct gttcgcgata cagcctgaac aatgcgagga cttcattggc ggttttagcc
      961 gatttcaatt ggctgacgag gcctggctcc tcaccaagcg acgccacagc ggccaacgcc
     1021 tcgagataac ggatggatgc ttcgggtggc gagacgataa gaaagatgag atggcagggc
     1081 ttgccgtcgg ctgcatcaaa atccatgccg gatggtgaaa tgcccagcgc taccgcgacc
     1141 tcgcgaactc cgtcagttcg ggcgtgcggg atggcaatac cgttcccaag tccggtgctt
     1201 cccagctttt cccggctcag gatcgcgttg agcagagcag cgcggtcaac ggctttgccg
     1261 ctgtcaataa gcaggtcgac aaggcgttcg atcagcgtcc atttgtccgg ctggccaaca
     1321 tcggccagga tcgtgttcgc cttgagcaat tctgtcaata ccataatttc acctccccac
     1381 ggtctgccgc gcggtcaatc ggcgctccgt atacatccag cgcccgcgcc attccttaaa
     1441 aagaccatgt attggactcc gtatgctata cgtatgtttc gggttcgggc gttcgcaaaa
     1501 aagtcgcgga tgcggcccgg cgtcaccgtg cgtcaatcag gcggagtcga aaaagtcctg
     1561 tatcacccgc cggccccgga ctaccagaac cggacatcgc gcaccacaga taatgcgttc
     1621 ggattcgttg aaggtcgtat cttttcggct taacgactcc tcaatctccc caaggatgat
     1681 caggttggcg ctgatttccc gggccttgtc gagcacctgg cgatgaacaa cgccgtgcag
     1741 aagctcagtg gtgacctcca gctctttttc ctgagcgaac cgctccatga gcgcaagata
     1801 ccgcctgcca tcgtcttcga gatcactttt gagagcgatc tcctcttctt tcagaaagac
     1861 atgggctctc gtaagttctt ccagaagccg ctcgtttatg acggcgatgg catggagttc
     1921 gcccccaaat tgtctggcaa aggcaaccgc atagcgaatg gcctgcaacc cgtgctcgtt
     1981 cgtttcaatg tagacgagaa tttttccaaa gtctgggagc gggggcatag gtcattcctc
     2041 ctcagcttcc aggcggtctt tcacgagctt gaggacaaag aaggcttccc ggtctgcctc
     2101 ttcaagcgcg gcttccacgt ggtgcaggtt gtcttcatag tcggggatga agatacgttc
     2161 cagggcgttg acgcggcgga tggtcttctg aatctctttc gcgaggcgca acacggagat
     2221 gcggcactcc gcgagcgcag ccagcgacgc cagcgcctct ttgaagcggc gaacggcctc
     2281 atcggtccac acggaggtgc cgctcgggcc gtaatacggc ggattcccgc tcatctggag
     2341 ttccacggcg ggcaggggga cgcccatcac ccggcgttcc cgaagcaaga ggcttgattc
     2401 gatgttcacg gcgcggctgg ccgagacgac cccggacacg ccggttacga attgagcctc
     2461 ctgcaatgcc tcgaaggccg ttgccattgc ctggctggtt ttttgttcgg cctcgatggc
     2521 gcgggcggtg aagcgcttga gttcgaggat caggatctcg cgtttctgct ccagcaattc
     2581 ataccccgag cgtgcaaagg caagctcgcc tttcaggcgc agcagattgg ttcgcgtcgg
     2641 agcaatgtgt ttcccggcca tattaccttc cgtaatactt cccgatttcc tctctcgtga
     2701 gacgatggag ttcttcactc ggcagcgact ggagaatttt ccagccgaga tccagcgtct
     2761 gctcgatcga gcgatcttcc tccagtccct gggacagaaa ctgccgctca aatgtctcac
     2821 caaacgccat atacttccgg tccaaatccg tcagttcttc ttcgccaatg acagacgcaa
     2881 gtgaacggat gtcctttaca tgtgcatacg aggcgaaaag ctggctggcg agattggcgt
     2941 gatcggcacg cgtcattccg tcgccgatgc cgtctttcat caggcgggac agcgagggaa
     3001 ggcctgcaat gggcggatag atgctccgtt gatcgagttc gcgctccagc acgatttggc
     3061 cttcggtgat aaacccggaa aggtcgggaa tcggatgtga aatgtcgtcg ttgggcatcg
     3121 tcaccacagg gatttgcgta atcgacccgg gtttgtcccg gagcatgccc gcccgctcgt
     3181 aaatgctggc cagatcgctg tacaaatagc ccggatagcc tttgcggctg ggaacttcct
     3241 gccgttgcgt ggagatctcg cgcagtgctt cgcagtagtt ggtcatatcg gtgaggataa
     3301 ccagcacgtg catccccagg tcaaatgcga ggtattcggc agcggtaagc gccgaccgcg
     3361 gggtcatgag ccgttccacg gacggggcat ccgccaggct gaggaacacg accacgcgct
     3421 ccatcgcgcc cgattcctca aagctgttga ggtagaaccg ggcctcgtca tgcttgatcc
     3481 ccatgccggc aaagaccacg gcaaacgggg tgtcttctcc gataatccgc gcctgccggg
     3541 taatctgagc gacaagctgg cggtgcggca ggccgctgcc ggaaaacacc ggcagcttct
     3601 gcccgcgcac cagtgtattc atcccatcaa tcgcggagat gccggtttgg atgaatttgc
     3661 gtggataagc gcgcgcggtg gggttgaccg gaagaccgtt gacgtcccgt tcctctccga
     3721 taagctccgg cccgccgtca aacggacggc ccaatccgtc gaaggcccgc cccaacattt
     3781 cctcggagac tgccagcgtg agtgggcggc cacgaaaacg cacgcgcgta tcgccaagtt
     3841 tgagccccga ggtaccctcg aaaacctgga tcacggcggc gccttccgaa acttcgagcg
     3901 tcattcccag gcgggtgttt ccttggcgat ccacaatttc ggccagttca ttgtagccga
     3961 tgttatgaat gccgctcacg cccacgatcg gtccggagac ttcgtcaagc cccacgtatt
     4021 cacggccatc caggagctgg cgttgggaga tttcagccgt agatgcttgt aagttcatga
     4081 atcgattcct cgagctcttt caacagtgca tcgagccggt ccaactcgtc gttgggacat
     4141 tcgtatttca tctgcataat atcgccaacg gcgtccatcc gcttcaggga cgccagtgta
     4201 acgcctttct gaatggcctg cgccgccaga tcgtgaaagg tcacgataat gttgagcatc
     4261 cggtattgtt ttccggcgac acagaacgcg tcgattttgt cgaaggcatt ctgttgcagg
     4321 aaggcgttct tgaacatgtt ggcggcctca aggatcagcc gctggctgtc gggaagcaca
     4381 tcggcgccga ccagtttgac gacatgctcc agtcgggctt ccttctgcag gatcgccatg
     4441 atggccgccc gcagctcccg ccatctgccg tttgttgcct catcccacca gggagccaca
     4501 tcgtcgatgt attcgctgta gctgtcgagc cagctgatcg cgggaaagtg ccgggcgttg
     4561 gccagttgct tgtccagcgc ccagaaggcg cgcacaaacc gcttcgtgtg ctgcgtaacc
     4621 ggttcggaaa aatcaccacc gggcggcgac accgcgccga tcgtcgtgat actcccttca
     4681 cgcccgctca gggcggtgac caggccggcg cgctcgtaga actcagcgag gcgcgttggc
     4741 agataggcgg gaaatccctc gtcggcgggc atttcttcca tccggccgga cagctcccgc
     4801 agggcttccg cccatcgcga cgttgaatcg gccatgatgg cgacgttgta tcccatgtct
     4861 cggtaatact cggcgatggt gatgcccgtg tagatgctgg cttcgcgcgc cgccacgggc
     4921 atgttcgagg tgttggcgat gagcagagtc cgttccatca acgacttgcc cgtgcgggga
     4981 tcgatcagtt cgggaaactc ggtgagcacg ccggtcattt cattgccacg ctcgccgcag
     5041 ccgatgtaca cggtgagatc ggcgtcacac cacttcgcga tcgcgtgctg caccatcgtc
     5101 ttgccggtgc cgaatccgcc gggcacgacc acgaccccgc cccgcgcgac agggaacagg
     5161 gtatcgagca cccgctgccc ggtgatcagc ggaccggcgc aggccaggcg tttggcatac
     5221 ggccggcctt tccggacggg ccatttgtgg aaaaggcaga gttcaacgtc tccgtcgggg
     5281 gtttttacgg tcgccacagc atcttcaatg ctatattcgc cttccggggc aagatgggtc
     5341 agcgtgcctt ccacccaggg aggaaccagg atccggtgct cgatgacttc tgtctcctgc
     5401 accactccca gaacggagcc gcccgagatc gggcacggag gcgtggcgca gggcgtgaaa
     5461 tgccagcgtt tctcccgcga cagcgcgggc gcatgaaccc ctcgcgacac gaaatcgccc
     5521 atctgagccc gcagcacatc gagagggcgc tggacgccgt cgtagatgct gcccagcagg
     5581 cccgggccca attccaccga cagcggttcc ccctggccaa tgatggaggc cccgggacgc
     5641 aggcccgtgg tgtcttcgta cacctggatc cacgccgctt cgccgcgaat ccgcacgatc
     5701 tcgcccacga gacgctcctc gcccacatag accagctcga gcatctccac gccggagaca
     5761 tggccggcct cgactaccgg gccaactacc cgtctcactg ttccgataat gcgctcagcc
     5821 atggctgttt attacatcct cgacgatcat cgcacgaagc tcccgtcgtt tacgctgcat
     5881 ccgtgttttg tacgtattgt catagacaat gcgcccgtct gttgactggg cgatacagcc
     5941 gccgcccgaa tcatgagaat ccagctgcaa tatcacacga gaaacgcccg cagctgcggc
     6001 gcgcggatcc gcagcgacct cgccggggaa cgcatcgctc aaaaacgcgg cgtcgtcctt
     6061 gtaaatccgc acgacgacct cgggcaatgc gatggcagcc gccgcttccg cggccaggtt
     6121 ccgcagcgac ttcgcgcaca tctccgggga ccgtctcacg gcctccaatt ttttctcgat
     6181 ctgggcgagc acgcgttccg ccacgctttc tcgagccgcc acaagaagcc gccgggcttc
     6241 gatccgggct gtggcgagtt cctgcgtgcg gagctttgca catcgctcac gggttgcttc
     6301 cagaacctcg tcgcgcccgc gttcgccttc catctgggcc tgtttccgcg cgtcagccgc
     6361 ggcgctttca gcgctttcga taatacgaga cgcctgggca tcggcatccg ccaggatgct
     6421 ctcgacaatc tgaaggcctc cggattccag ggcttcatcg ctcatagatt gactcccacg
     6481 gccttgcgga cgatttccgc caggccgggc ctgtcgggca gcgggcccga ggcatcgggc
     6541 acttcaagga tcaggggaaa cccggcgtcg gaaaggcgcg catcgatttc atcgcggatc
     6601 tccactgcaa ggcgttcaga taccacaata agcccaattt cacgccggcc gagcgcctcg
     6661 ttcagtgcgg ccagcatctg atcccgtccg ctggcaacgc ggccctcaac gccaaccagg
     6721 cgaaatccga gaacggtctg gtcgtctcct atgacaaagg ggatcatgtc acaccttgcc
     6781 cagcaccata atgccgatga tcagcccgta gatcgcgata ccttccgcca acccgacaaa
     6841 aatgagcgtg cgggcggcaa gttgcggttt ttcggcaatg gctccaacgg cggcgctgcc
     6901 cacataggca accgcgatgc ccgcgcccac acagcccagg ccgacactca acgccgcggc
     6961 gagataggcc cagccagcgt tcccggaatc gggggaaggc gcggcctgcg cggtctccgg
     7021 ctgtgcccag gccccggagc accagagcaa cgccgcaagc atcacaacgc tcgccagcag
     7081 caaggcccgg acagcccgtt tcgaatactg gattcccttg tacatcggtt ctgcctttca
     7141 gtttgtcgcg tctgacggcg gcggcaccgc cccgacgggc gcgtatttct gcccaacccg
     7201 gttgaaaaac ttgccgaaaa attcatagta ctcgaggcgc agcgcctgga tggtgaccac
     7261 gacgccttcg agcacgatga tgagcacgtt gcctgcgacg atcacggcca gcgataagaa
     7321 acggccgccc ggcgccccgt ggacgatatc cgccaggctg aacacggcga caaacagtcc
     7381 ggcatgtgct agaccgaacg ccgccacacg aatgaaggac accgtattcg ccagatatcc
     7441 catgaccact tccatgagct ccacggcgga ttccatgaca taggtgatga cgccttccgg
     7501 aaacgcccgc tcctgttttc cggaccattt cagaagaggg cctttcatga gaaatccgag
     7561 cagcgctatc gccatcggaa ccaccagcca tgccgaatgg ggcatgcccg aggagttcag
     7621 gaacgatttc acgaccagcc cgatgccgga ccagtagatg atcccggcga gagggcccgc
     7681 gccatcaaac agggcgttcc agaacgcgcg tgcgcggatc gcgttcaaga tattcaacac
     7741 gatgcccaga ctgacgaaca ggaccccgaa tccgatagcg aaggcaaaca gttcgt
//
LOCUS       DAOPBQ010000101         7782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8513_length_7782_cov_4.275657, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000101 DAOPBQ010000000
VERSION     DAOPBQ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8513_length_7782_cov_4.275657"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKW60_04790"
     CDS             complement(<1..209)
                     /locus_tag="PKW60_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98779.1"
                     /translation="MVRKFTAWALLLGFFLSAGCGERGAPVIPPGMVPVGEHPLEINT
                     PPLKVVARITEPFPEETASAADELDA"
     gene            537..2891
                     /locus_tag="PKW60_04795"
     CDS             537..2891
                     /locus_tag="PKW60_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPJ98780.1"
                     /translation="MKGWRGISAILAAGVLLFSISYYPGYFLGNELWGDFFEFIGTGL
                     LVFGACAAARGLTRKSAMVYFLYIGAVCLAFSRFIDFTFEISAIASWPVVGSEGFGHK
                     LAPRITENAGYISILLAFLALLRDVTRLHDHLEKDHLRLKDLHDQGVFLARVADMSAE
                     AVFGCDRDGVIRTWNRAARSLFGYAAEEAIGMPVARLFAGDAAPLSLMSREQVEEGAP
                     LRDLELAAQTKSGQKFWASASISTVSEDEGAPLGFSVIVRDIEARKEAERKLRHSHDM
                     LASALHNAEVGMLIIDRNLELMAFNPRLEEIVGGKVTDFQTLRDACGRALDPQVPFLE
                     RLTKDVLEQGRPIEFRNLAIHRRDGADRICNIAFSPMMEDEGGVNGAVCIVVDVTERE
                     ALQARLIESQKMESLGRLAGGIAHDFNNILGGILGYASLLRETSPAGSDFSRYARAIE
                     GAASRAAELTQQLLAFARGKKTEAQETNLNAVIDETLSLLSLAVDPSIELRFERARDL
                     YSAKADLSQINQMLMNLLINARDAIEDRGRICITTANVDVDAEMRERLNLDRLGPYVH
                     VTVEDSGAGMPPEVVRHIFEPFFTTKTNTNGYGLGLSVVYGVVEAHGGAIRVDSELGR
                     GTRIDVYLPAFESVDTGPREQPRKPAARNGGEHRHETILIVDDELFMQNLLRDLLEAE
                     GHTVLAADTGEQSIEVFDEHGAIISLVVMDLLMPGMGGAAAIKCLRERAPELPCIISS
                     GYGTDTVEPALQQDERIRFVPKPYRAMDMVSAVRDLLDSCAQIS"
     gene            3187..3879
                     /gene="pspA"
                     /locus_tag="PKW60_04800"
     CDS             3187..3879
                     /gene="pspA"
                     /locus_tag="PKW60_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HPJ98781.1"
                     /translation="MGIFSRVADIISSNINAMLDKAEDPEKLVRLMIREMEDTLIEIK
                     ASCAGAMAQGKKIGRQLEEARAGEMRWTERAQLAVDKGRDDLAREALLEKRRFTHRAE
                     ALEQELVQFESLVEQYQTDIIQLEEKLATARNKERMLAQRHVHAQHKKRAQQNIRKAD
                     YTNAVARFENLESRIDRMEAGAELVNFGRKPALGEEIDRLATDEALEAELNALKSPET
                     GPKKAEIAGELE"
     gene            3910..4263
                     /locus_tag="PKW60_04805"
     CDS             3910..4263
                     /locus_tag="PKW60_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98782.1"
                     /translation="MKALICTGVAIALLLVLLTKVVSGVVMAFSLLPHVLTGLGILLA
                     GFVLGLLVARRHLPGGQRPRGDAARRSEIMANYKIHAGLDCMEGRIEALEARLFDGAP
                     PGRIGCAGSGIGRIA"
     gene            4263..4529
                     /locus_tag="PKW60_04810"
     CDS             4263..4529
                     /locus_tag="PKW60_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98783.1"
                     /translation="MHSFSLTLVALAVVLIPLLALAVLVLLVLGLVRIFTGQPIRTSP
                     ALEAQEAALMQELHRNMARLEERLENIETLVLEREGHKSDSLRK"
     gene            4567..4695
                     /locus_tag="PKW60_04815"
     CDS             4567..4695
                     /locus_tag="PKW60_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98784.1"
                     /translation="MDTVWTAAMALAGHSHSLGLAVVLPFLGPAAAVAGLRPAAGD"
     gene            4698..5081
                     /gene="pspC"
                     /locus_tag="PKW60_04820"
     CDS             4698..5081
                     /gene="pspC"
                     /locus_tag="PKW60_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HPJ98785.1"
                     /translation="MNPHYEPQTKGLYRSRQGLFFGVCRGVSDYLDVSVFWARVIGVL
                     LLFFTGIWPVLGLYILAALLMKPAPVVPFQSIEDAEFYNSYTSSRSMALYRLKRTFDN
                     LDRRIQRIESIVTARDYNWERRLRE"
     gene            complement(5112..6533)
                     /locus_tag="PKW60_04825"
     CDS             complement(5112..6533)
                     /locus_tag="PKW60_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98786.1"
                     /translation="MRTVLCVTIFMLGVAGVFAAESTPIQVDGVFPNMTVFAPGAGSR
                     SETGIGALIPWADRLWAVGYVAHIAGDGIGLYEIRDDMTMHLHPASVTGTFANRLVHW
                     ETLQAVIGPHVIDAEGRVRTIDALAKHRLTATARHLVHPKTMVYFLTMEGLLFETDLL
                     TLETRELADVTAELGIEGQPHFKGAHTAQGRLVVANNTYEEPEFLGTRASGCLGEWDG
                     DRWRVLERNPFVEVSGKQNPSPGERYGNTLFATGWCKSAAILRVLQNGTWSRYLLPKA
                     SHAFDHTWNTEWMRIREAQTERYLMDLHGMFYELPALIYDGRIWGIRPISTHLRIVPD
                     FCHWRGLFVMASDQTDNAVGQPQSGLWFGNIDSLWSMGKPSGWGGPWWDTPVTPDAAS
                     DPFLMTGFDKKCVHITHQAREAVSFTLEVDFLGNGSWQTYDSLAVAPGSYFHHEFPDA
                     FSAHWIRIRVDRPCTATVYFTYS"
     gene            7211..>7782
                     /locus_tag="PKW60_04830"
     CDS             7211..>7782
                     /locus_tag="PKW60_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98787.1"
                     /translation="MRFSGAFFFVVAVAVSASGQMVFVEAEGFEELGGWVVDQQAMDQ
                     MGSPYLLAHGLGEPVKDAATDVLFPEPGAYRVWVRTRDWVASFGAPGAPGRFQVQVDG
                     VPLEPVFGTEGAAWHWQDGGLIEIAGPRVRLSLHDLTGFEGRCDALLFTKDTAFVPPD
                     DGEALLSFRRAALGLPERPQPTLADLDDLVG"
BASE COUNT         1522 a         2344 c         2483 g         1433 t
ORIGIN      
        1 gcgtccagct catccgcggc gctggcggtc tcctcaggga atggctccgt gatccgcgcg
       61 accaccttca aaggcggcgt gttgatctcc aacggatgct ccccaacggg caccatgccg
      121 ggaggaatga cgggcgcccc gcgctctccg catcccgcgg acaggaagaa gcccaagagc
      181 agcgcccatg ccgtaaattt ccgcaccacc agaactccag atttggggga cacaaggcgc
      241 aattatactc atggcagggg cgatttcgca ccttgatccc tgcccgggct gcctccgtta
      301 gtcgcaacag caacaaaatg cggtgtagta cagaagtaca aaaatctgag cggtgcgcgg
      361 cgcattaaac cgtaaacggc gcatatgatg ctataattcc attcaaatgc cctgatacgt
      421 gctctgacga agttggcacg aacgcggttt tcagactata ctctaagggg gctgggaccg
      481 ggcgggcgaa tccgaacaga gcgcaggggg catctgtttt cagacggaga gggcgaatga
      541 aaggctggcg gggcataagc gccatcttgg cggccggagt gctgctcttc agcatctcgt
      601 attatcccgg ctatttcctg ggtaacgagt tgtggggaga cttcttcgaa tttatcggaa
      661 ccgggttgct cgtattcggg gcgtgcgcgg ctgcccgcgg attgacgcga aaatcggcca
      721 tggtgtactt cctgtacatt ggcgcagtat gtctggcttt ctcacgattc atagatttta
      781 cattcgaaat ttccgcgata gcctcctggc ccgtagtggg cagcgaagga ttcggacata
      841 aactggcgcc ccggatcacc gaaaacgccg gttatatctc catcctcctg gcgtttctgg
      901 ccctgctgcg tgatgtgacc cgccttcatg accatctgga aaaagaccac ttgcgactca
      961 aagacctgca tgatcagggc gtgtttctgg cgcgcgtggc cgacatgtcg gcggaggccg
     1021 tgttcgggtg cgaccgggat ggagtcattc gaacctggaa ccgcgcagcg cgctcgttgt
     1081 tcgggtacgc agcggaagag gccatcggga tgccggtagc ccggctcttc gccggagacg
     1141 cggcgcccct gtcgttgatg tcgcgcgaac aggttgaaga gggcgcgccg ctgcgggatc
     1201 tcgagctggc cgcacaaacg aaatccggcc agaagttctg ggccagcgcc tcgatttcca
     1261 cggtgtcgga agacgaaggc gcgccgctgg gtttcagtgt cattgtgcgc gatatcgaag
     1321 cacgtaaaga ggcggagcga aagctccggc actcgcacga catgctcgcc agcgcgctgc
     1381 acaatgccga agtgggcatg ctgatcatcg accgcaatct tgagttgatg gcattcaacc
     1441 cccgcctgga agagattgtc ggcggcaagg tgaccgactt tcagacgctt cgcgacgcat
     1501 gcggccgggc cctggatccg caggtgccgt ttctggaacg cctgaccaaa gatgtgctgg
     1561 aacaaggccg gcccatcgaa ttccggaacc tggccattca ccggcgcgac ggcgccgacc
     1621 ggatctgcaa catcgcgttc tcgccgatga tggaggatga aggcggagtc aatggcgcgg
     1681 tgtgcatcgt tgtcgatgtc accgaacgtg aagcgctgca ggcgcggctg atcgaatcgc
     1741 agaaaatgga gagcctgggc cgtctggcgg gcggtattgc ccatgatttc aacaacatac
     1801 tcggaggaat cctgggctat gcgtcgctgt tgcgcgagac gtcgcccgcc ggctcggatt
     1861 tctcccgtta cgcccgcgcg atcgagggag ccgcctcgcg cgcggccgag ctcacccagc
     1921 aactgctcgc cttcgcccgc ggcaagaaga cagaggccca ggagaccaat ctcaacgcgg
     1981 tcatcgacga gacgctctcc ctgctgtcgc tggccgtcga ccccagcatc gagctccgtt
     2041 tcgagcgcgc ccgtgacctg tattcggcga aagccgacct cagccagatc aaccagatgc
     2101 tgatgaacct gctcataaac gcgcgcgacg ccatcgagga ccgggggcgg atttgcatca
     2161 caaccgccaa cgtggatgtg gatgctgaga tgcgagagcg cttgaacctt gaccgcctcg
     2221 gaccgtatgt gcatgttacg gttgaagata gcggtgccgg catgcccccc gaagtggtgc
     2281 gccacatttt cgagcccttt ttcacgacca agaccaatac caacggatac ggcctgggcc
     2341 tgtccgtggt ctatggcgtg gttgaagcac acggcggcgc catccgggtc gattctgagc
     2401 tcgggcgcgg tacccgcatc gacgtgtacc ttcccgcctt cgaaagcgtc gacacgggcc
     2461 cacgcgaaca gccgcgcaag ccggcggcgc gaaacggcgg agagcacaga cacgagacca
     2521 tcctcatcgt ggacgatgaa ctgttcatgc agaacctcct gcgcgacctg ctcgaggcag
     2581 agggccacac cgtgctggcc gcggataccg gcgagcaaag catcgaggtc tttgatgaac
     2641 acggggcgat catctctctc gtggtcatgg acctgttgat gccaggcatg ggcggcgccg
     2701 ccgccattaa atgcctgcgt gaacgcgccc ccgaactgcc gtgcatcatt tccagcggct
     2761 atggcaccga caccgtggaa ccggccctgc agcaggacga gcgcatccgc ttcgtgccca
     2821 aaccataccg cgccatggac atggtgtcgg ccgtgcgcga cctcctggat tcctgcgccc
     2881 agatctcctg agcacccgcc aggcgccagg ccacggcggg ctttcaccgc cctcctcgca
     2941 aacacacggc aagctcgcgg catgtggtaa aattcgccac acatgggatg aattaacctc
     3001 aaaactgtta catttgccgc gaaggatgtc tagactttcg cctcaattcc aaattaaccc
     3061 attgatttac tgtgctttac agcttttgac ggtcttggca cagaggttgc gctttcctcg
     3121 gcatgttttg aaccgggagc catttcaacg gggttccccg ctggtttcag ccggaggtga
     3181 cacgatatgg gtatcttttc gcgcgttgcc gacattatca gctcaaacat caacgcgatg
     3241 cttgacaaag ccgaggaccc ggagaagctc gtgcggctga tgatccggga gatggaggac
     3301 accctgatcg agatcaaggc ctcgtgcgcg ggtgcgatgg ctcagggaaa gaagatcgga
     3361 cggcagctcg aggaggctcg cgcgggcgag atgcgctgga cggaacgggc gcagctggcc
     3421 gtggacaagg gccgggacga cctcgcccgg gaggcgctgc tggagaagcg ccggtttacg
     3481 caccgcgcgg aggcgctcga gcaggagctg gtgcagttcg aatcgttggt ggagcagtac
     3541 cagactgata taatccagct cgaagagaaa ctggccacgg cgcgcaacaa ggagcgaatg
     3601 ctggcgcagc gccacgtcca cgcgcagcat aagaaacgcg cccagcagaa cattcgcaag
     3661 gccgattaca cgaacgcagt ggcccgcttc gaaaatctcg agtccagaat cgatcgaatg
     3721 gaggccggcg cggaactggt caattttggg cgcaaacccg ctctcggcga ggaaatcgac
     3781 cgtctggcca ccgacgaagc gctcgaagcc gaattgaacg cgttgaagtc gccggaaacc
     3841 ggcccgaaga aggccgaaat cgccggcgag cttgagtaac ccggcatcgc tcagcggaga
     3901 agccgaacca tgaaagcgct tatctgcaca ggcgtggcca tcgcactgct gctggtgctg
     3961 ctgacgaagg ttgtgtctgg cgtggtgatg gcgttttcgc tgctccccca cgtgcttacc
     4021 ggcctgggga tcttgctggc gggttttgta ctgggcctgc tcgtggccag gcggcacctg
     4081 ccgggcgggc aaaggccccg gggcgacgcg gcacgacgga gcgaaatcat ggcaaattac
     4141 aagatccacg ccgggctgga ctgcatggaa ggacgcattg aagccctgga ggcgcgcttg
     4201 ttcgacgggg caccgcccgg ccgaataggc tgcgcaggct ccggaattgg gagaatcgcc
     4261 tgatgcacag cttctcgctg accctggttg cgctggcagt cgtgttgatc ccgctgttgg
     4321 cgctggcagt actggtgctc ctggtattgg ggctggtgcg tatctttacg gggcagccga
     4381 ttcgcacgag ccccgcgctg gaagcccagg aagcggcgct tatgcaggag ctgcaccgca
     4441 acatggcccg gctggaggag cggctcgaaa acattgagac cctcgtgctc gaacgggaag
     4501 gacacaaatc ggattcgctc cgtaaataat cctgcccgtg ctggacacag cggaacggag
     4561 tggcgtatgg acaccgtatg gacggcggcg atggccctgg cagggcattc gcacagcctg
     4621 gggctggcgg tggttttgcc ctttctgggg cctgcagctg ccgttgccgg gctgcggcct
     4681 gccgcaggag actgactatg aacccgcatt acgagcctca gactaaaggc ctgtaccggt
     4741 cgcgccaggg cctctttttc ggcgtgtgcc ggggtgtctc cgactacctc gacgtatccg
     4801 tattctgggc gcgggtgatt ggggtgctgc tccttttctt tacgggcatc tggccggttc
     4861 tcgggttgta tattctcgcg gcgctgttga tgaaaccggc gcccgtggtg cccttccaga
     4921 gcatcgagga tgcggagttc tacaactcgt acacatcgtc gcgttcgatg gcgctgtacc
     4981 gcctcaaacg cacattcgac aacctcgatc ggcgaatcca gcgcatcgag agcatcgtga
     5041 ccgcgcgcga ttacaactgg gagcgccgcc tgcgcgagta acgccctgcg cggccgcggg
     5101 ccgcgccccg ctcacgaata cgtgaaatac acggtagcgg tgcagggacg atccacacgg
     5161 atccggatcc agtgagccga gaacgcgtct ggaaattcgt ggtggaaata ggaaccgggc
     5221 gcgacggcca gggaatcgta cgtctgccat gagccgttgc ccagaaaatc cacttccagt
     5281 gtaaaactga ccgcctccct ggcctggtgc gtgatgtgca cgcatttctt gtcgaatccg
     5341 gtcatcaaaa aggggtcgga cgctgcgtcc ggcgtcacgg gcgtatccca ccaggggccg
     5401 ccccagccgg aaggtttccc catggaccag agggaatcga tgttgccgaa ccacaggccg
     5461 gactgcggct ggccgacggc gttgtcggtc tggtcgcttg ccatgacgaa caggccacgc
     5521 caatggcaga aatcaggcac gatgcgcagg tgcgtcgaga tgggccgaat gccccagata
     5581 cgcccgtcgt agatgagcgc gggcagttca tagaacatcc cgtgcagatc catcagatag
     5641 cgttcggtct gtgcctcgcg gatgcgcatc cactcggtat tccacgtgtg atcgaaggcg
     5701 tggctggctt tggggagcag gtagcgggac cacgtgccgt tctgcagcac gcgtaaaatg
     5761 gccgccgact tgcaccagcc cgtggcaaac agcgtgttgc cataccgctc gccgggagaa
     5821 gggttctgtt tgcccgacac ctcaacaaag ggattccgct cgagaacacg ccaccggtcg
     5881 ccgtcccatt cgcccaggca tccggacgcc cgcgtgccca gaaattcggg ctcctcgtag
     5941 gtgttattgg ccaccaccag gcggccctgg gccgtgtgcg ctcccttgaa atgaggctgg
     6001 ccttcaatgc ccagctccgc ggtcacatcg gccagttccc gcgtttccag ggtcagcaga
     6061 tcggtctcaa agagcaaacc ctccatggtc aggaaataga ccatggtctt gggatgtacg
     6121 agatggcggg cggtagcggt gaggcggtgt ttcgccaggg cgtcaatcgt gcgcacccgg
     6181 ccttcagcat cgatgacatg cgggccaatc acggcctgga gcgtctccca gtgcaccaga
     6241 cggttcgcga aggtgcctgt gacggaggcc gggtgcaggt gcatggtcat gtcgtcgcgg
     6301 atttcataca ggccgatgcc gtcacccgca atgtgtgcga catagcccac tgcccacagg
     6361 cgatccgccc acgggatcag cgcgccgatg cccgtctcgc tgcggcttcc cgcgccgggc
     6421 gcaaacaccg tcatattcgg aaacacgccg tccacctgaa tgggagtgct ctcagctgca
     6481 aaaacgcctg caacccccag cataaatatc gtcacacaaa gcacggtgcg catggtcaac
     6541 ctccgggtac atcaacacaa acggctgttc acgcccggta tattaggcat gtggaggaag
     6601 tgcatcaagc cttcgaaatc cgtcatttgc gatctcgccg gtgcatggaa cgggtgcatg
     6661 gaacacagga cgacgccggg gaagcaattt ttggatggtt caggcaagta gcggatgcgc
     6721 caagcaacgc ggcattgcgg gccattccgc gcctgcggat gagttctgtg ctctgacctg
     6781 gccaacccag gtttgcgcag cgcgcagggc caggcccagg tgtctgccct tcttcaaggc
     6841 agtcgcaggg gctatccgga tgggcggcca aagggggcca cccctgcaat ccggagggga
     6901 accgcgccac gtccgccagc gccctgcggg gaacacagaa atccgctgcc cgagaaatat
     6961 ttggatgcaa cgcacctgtc ccccgctggc gggggtgaat cggcgatagc cgattcgggg
     7021 gtggacagcg gcctcaaccc cacggcaaac ctcccatgct tgacagaaaa accatggacg
     7081 cagcaataca ccccagccca attcgcaaat cccaccacgc ccgaggcggc ggctcgtttg
     7141 cgaaggctgc gcgggcccgc taccatgcgc tccaaggtag tacatttctc gtaactccgg
     7201 ggagcatgct gtgcgatttt caggggcatt cttttttgtt gtggcggttg cggtatccgc
     7261 gtcgggccag atggttttcg tcgaggcgga gggttttgag gagctgggcg gctgggtcgt
     7321 tgatcagcag gcgatggacc agatgggttc accgtacctg ctcgcgcacg gcctgggcga
     7381 gccggtcaag gatgccgcca cagacgtgct gttccccgaa ccgggcgcct accgggtatg
     7441 ggtgcggacg cgcgactggg tggcgtcgtt tggcgctcct ggcgcacccg ggcggtttca
     7501 ggtgcaggtc gacggcgtgc ctctggagcc cgtcttcggg acggaagggg ccgcctggca
     7561 ttggcaggac ggcggcctca tcgagatcgc cggtccgcgc gtccggctgt cgctgcatga
     7621 tctcacgggg tttgaagggc gctgcgacgc gctgttgttc acaaaagaca cggcattcgt
     7681 cccgccggat gacggggagg cgctgctgtc gtttcggcgc gcggcgcttg gcctgccgga
     7741 acggccgcaa ccgacgctcg ccgacctgga cgacctggtc gg
//
LOCUS       DAOPBQ010000102         7779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8518_length_7779_cov_4.363801, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000102 DAOPBQ010000000
VERSION     DAOPBQ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8518_length_7779_cov_4.363801"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKW60_04835"
     CDS             <1..614
                     /locus_tag="PKW60_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polyvinylalcohol dehydrogenase"
                     /protein_id="HPJ98788.1"
                     /translation="CAVGGIVGVAADDPDAGQILWELPWKANVVAPSPVPLGDGRLFC
                     TAGYGNGSLMVQLHKEGGAFRAEAVFERGPSEWLACEQQSPIYYDGLLHAIMPKDAGA
                     LRGQFVCYDPDGSLVWSSGQANRFGLGPFILADGKFYVLSDDGILTVLQQSREHYRAL
                     AQVRVLDGQDAWGPIAVAGTRMLLRDSTRMVCINASAATGEAM"
     gene            617..1630
                     /locus_tag="PKW60_04840"
     CDS             617..1630
                     /locus_tag="PKW60_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98789.1"
                     /translation="MSAENHRVIPLWVWVAIAFVSVGAAVWLASSGRRPDPPESFRYD
                     VAEFEQVDPGRLIATELAPIAPGIPGLRAIAATPDGRILIAGDKTLLTCGLDGSEQTR
                     SSLTATPVCVAAAPNGQVFLGMVDRIDVLDAAGKSDGSWPLPGDRGHVTSIAADEDSV
                     YVADAGNRAVLRFDYGGQLLARIGEKDAARDIPGLIVPSPYFDVAFDNAGGLWAVNPG
                     RHGFEQYRATGELVSSWYRPSMEPDGFCGCCNPAHIAFRSDGTMVTVEKGLVRVKLYS
                     VDQKLLGFVAGPDAFRSTPGGPFSAELDMPLLDVAVDANDRILIVDGRSNTVRVFKIE
                     EPA"
     gene            1632..1835
                     /locus_tag="PKW60_04845"
     CDS             1632..1835
                     /locus_tag="PKW60_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98790.1"
                     /translation="MERPRVERREFFAGLARACLLGGLGWTGGLLAWRRWKAGACADL
                     RLCRACPIHTACPDARRRIEGKP"
     gene            1832..2413
                     /locus_tag="PKW60_04850"
     CDS             1832..2413
                     /locus_tag="PKW60_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPJ98791.1"
                     /translation="MSTDKAIRRRDFIRAAAALGVGGALWKAAAPASGATVWQIDPQK
                     CVQCGRCATECVLNPSAVKCVHSYQICGYCDLCGGYLQPSAKARTTGAENELCPMSAI
                     SRTFIEDPFFQYIIDESLCVGCGKCVGCGKCVKGCALFGNSSLYLQVKQDLCVHCNEC
                     AIAKACPGDAYRRIPAEEAYLLKTRTEGGNAQE"
     gene            2410..3696
                     /locus_tag="PKW60_04855"
     CDS             2410..3696
                     /locus_tag="PKW60_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPJ98792.1"
                     /translation="MKLLKTLTPVLAAAALFGGVAWAEFRFPMPEFQSGYTYPEMHTP
                     MPGRTNDLWDTVLLAAALGLAAWLVLKRRSRPAVFGLTLCSLAYFGFWRQGCICPVGS
                     MQNVAEGILGVEVVSIAVLSFFLLPLIFALFFGRVFCAAVCPLGAIQEVVAVRPVMLP
                     RAVDRVLGFVPYIYLALTILAVSTGSGYLICRYDPFVGFFRLGASLNMFLAGGVLLLT
                     GLFIGRPYCRFLCPYGVLLGWMSRFSKWHASITPRECIQCRLCENACPYGAINIPTPD
                     KTPLTAKEGARKLGVLLALTPILIAIGALTGLFSYPMLARIHPTVQLASRIAGEEAGR
                     YQGMTIESEAFRAGVKTQDELYAEAHAIRADFKWKTALCGAFLGLVIAVTLASLSMAG
                     CRDDYETDRRACVSCARCFPYCPVEKDEVAVAEQKT"
     gene            3677..5440
                     /locus_tag="PKW60_04860"
     CDS             3677..5440
                     /locus_tag="PKW60_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPJ98793.1"
                     /translation="MQNKRPEFVPSQAKWRIASAVAAVAGLFTLVLASLLIINYLQIQ
                     ATDPLDNPELLKLREQLAASSGNNEALVEQIRTLDLLVRKAFFTSQAQLQLGGRLLLA
                     GAIVFLVAFRLAARWNPVLPSPVGTPDTMKYWRILAQSRELITGVAVVLVLVTLAAAY
                     VTPFDIPAPDLEKQAPEGAAEAETVSDAEAVDFPTWEEMLAQWPSFRGPGGYGVAHFT
                     TAPVNWNLEAGEGIRWAVDAPQGGFNSPVVWDNRLFLSGASKELREVYCYDTETGELV
                     WRQAVPAFPGTPETLPRVSQDTGYAAPTMALLGDRACVIYATGDIACFDFDGGLIWGR
                     NLGVPENHYGHASSLIAYAGRVYVQLDDSKQPRLLALDAATGEDVWIAERKKISWASP
                     ACAPTPFGFQLLLASEYDVDGYDPATGSLLWTQKGLDGEVAPSPTISGDVVFVGNEYA
                     MATAVRLTSSNEGVQSEIIWQWEDSLPEVASPTSCDECFYIATSLGEIICLDKDTGEE
                     AWLHEFDQGFYSSPIRVGDRIYAADLKGTMQIFRAGREFEPIAALDLGEPVYATPAYL
                     DGRIYVRTESRLLCIEDNAAH"
     gene            5427..>7779
                     /locus_tag="PKW60_04865"
     CDS             5427..>7779
                     /locus_tag="PKW60_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPJ98794.1"
                     /translation="MLHTEFCIDDVDRLVAEIGHGREAVIPILHALQDRYRYLPDAAL
                     ARVCEITEITPATIEGVATFFTRFRRRPMGEHVIRVCDGTACHVKGAPAVFDAFAKAL
                     DLSPGEDTDKQGQFTVQRVACLGCCTLAPAVQIDHVTYGHVESEKVSYLLEDFLANEA
                     NGKAAPGRRQAESAPAAGEIRVGLGSCCIAGGSEKVRVALEDALQTIAGSVRIKRVSC
                     VGMCHQTPLLEVHLPGRPPSLYAKVQAEDVGEIVRRHFAPRKHSVRIRTDIGHWLRRL
                     YTGERRTVLERHGIDVRDRPVAAFLSKQQRLATEYCGEIDPVDLSEYLRLRGFEALAR
                     CLPQPAPAGDDSIAHLTPDAIIAELRESGLRGRGGAGFPTAQKWEIVRAAGDMPKYVI
                     CNGDEGDPGAFMDRMILESYPFRVIEGMIIASVAVGAHEGFLYIRAEYPLALARIEEA
                     LHIAEQAGYLGDNILGSGHAFRMQIIEGAGAFVCGEETALLAAMEGRRATPHIRPPYP
                     AVAGLHGHPTLVNNTETFALVPWIIRHGAAAFAAFGTAKSRGTKVFSLAGKVNRGGLI
                     EVPMGTTIREIVEDIGGGVQGGKQFKAVQVGGPSGGCIPRALADTPVDFEALATAGAM
                     MGSGGLVVLDETDCMVDMTRYFLSFTQLESCGKCTPCRVGTKRMLEIVENLCEDKAEP
                     KDLAELETLARVVKDNSLCGLGKTAPNPVLTAMQYFRDEFEAHAQGRCPAGKCKSLIT
                     YSITENCIGCTKCAVNCPSGAIEFRPYQQHEIDPALCTRCNGCY"
BASE COUNT         1353 a         2313 c         2578 g         1535 t
ORIGIN      
        1 actgcgctgt cggcgggatc gtgggggtcg ccgcggatga tcccgatgcg gggcagattc
       61 tctgggagct gccgtggaaa gccaacgtgg tggcgccatc gcccgtgccg ttgggggatg
      121 gacgtttgtt ctgcactgcg ggctacggca acggcagcct gatggtccaa ttgcataaag
      181 aaggcggggc gttccgggcg gaggccgtgt ttgagcgggg cccttcggaa tggctggcct
      241 gcgaacagca gtcccccatc tattacgatg gtttgcttca tgctatcatg ccgaaggatg
      301 cgggcgcgtt gcgcggccaa ttcgtctgtt acgacccgga tgggtctcta gtatggtcca
      361 gcggccaggc caatcgcttt ggtctggggc cattcattct cgcggacggg aagttttacg
      421 tcctcagcga tgacggcatc ttgaccgtgc tgcagcagag ccgcgaacac taccgtgccc
      481 tggcccaggt gcgcgtgttg gatggacagg acgcctgggg gccgattgcg gtcgccggta
      541 cccggatgct cttgcgggat tccacgcgga tggtgtgcat caacgccagc gcggccacag
      601 gggaggccat gtagccatga gtgctgaaaa tcatcgtgtg attcccctgt gggtctgggt
      661 ggccatcgcc tttgtgtcgg ttggcgcggc cgtttggctt gcttcgagcg gcaggcgtcc
      721 tgacccgccg gaatcgtttc gatatgatgt cgccgaattt gagcaggttg accctggccg
      781 cttgatcgcc accgagctcg cgccgatcgc ccccggcatc cccggactgc gcgccatcgc
      841 ggccactccg gatggccgca tcctcatcgc gggcgataag acgctgctga cctgcggtct
      901 agacggctcc gaacagacgc gcagttccct cacggccaca ccggtatgcg tagccgccgc
      961 gcccaacggc caggtgtttc tggggatggt ggatcgcatt gacgtacttg atgccgcagg
     1021 taaatcggac ggctcctggc cgctcccggg ggatcgcggt catgtcacgt cgatcgcagc
     1081 cgacgaagac agcgtgtatg tcgcggatgc gggaaaccgc gccgtgctgc gctttgacta
     1141 tggcggccag ctcctggctc gcattggaga aaaagacgcc gcccgggata tccccggtct
     1201 gatcgtgccc agcccgtatt tcgatgttgc gttcgacaac gcgggagggt tgtgggcggt
     1261 caatcccggc cggcacggct tcgagcagta tcgcgcaacc ggcgagctgg tctcctcctg
     1321 gtaccggcct tccatggaac ccgatggctt ttgcgggtgc tgcaatcccg ctcacatcgc
     1381 attccgttcg gacggcacga tggtcaccgt tgaaaagggt ctggtccggg taaaactgta
     1441 ttcagtcgac cagaaactgc tgggatttgt tgccggcccc gatgcatttc gaagcacgcc
     1501 gggcgggccg ttctctgcgg agctggatat gccgctgctc gacgtggccg ttgatgcaaa
     1561 cgaccggata ctgattgttg atgggcggtc gaacaccgtg cgcgtattta aaatagagga
     1621 gccggcctga aatggaacga ccccgtgtag aacgccgtga gttctttgcc ggactcgccc
     1681 gggcctgtct cctgggtggc ctcggctgga ccggcggcct gctggcctgg cgccgctgga
     1741 aagcaggcgc ctgtgcggac ctgcgcctct gccgcgcatg cccgatccat acggcgtgcc
     1801 cggatgcgcg ccgccgtatt gagggcaagc catgagcacg gacaaagcaa tccggcgccg
     1861 ggatttcata cgggctgcgg cggcccttgg cgtgggcggt gcgctctgga aggccgcggc
     1921 gccggcgtcc ggcgcgacgg tctggcagat cgatccccag aaatgcgtgc agtgcgggcg
     1981 ctgtgcgacc gagtgcgtgc tcaatccgtc ggcggtcaaa tgcgtgcact cgtatcaaat
     2041 ctgcggctat tgcgacctgt gcgggggcta tcttcaaccc agcgccaaag cccggaccac
     2101 gggagcggaa aacgagctct gcccgatgtc cgccatcagc cgcaccttca tcgaggatcc
     2161 gttctttcaa tacatcatcg acgaatcctt gtgcgttggc tgcgggaagt gcgttggctg
     2221 cgggaagtgc gtgaagggct gtgcgctgtt cggtaacagt tcgctctatc tccaggtcaa
     2281 gcaggatctc tgcgtgcact gcaacgagtg cgcgatcgcc aaggcctgtc cgggcgacgc
     2341 ctaccggcgc atcccggccg aagaggccta tctcctgaaa acacggaccg agggagggaa
     2401 cgcacaggaa tgaagctcct gaagaccctc actccggttc tcgccgccgc agcgctcttt
     2461 ggcggggttg cgtgggcgga attccggttt cccatgccgg aattccagtc cggatacacg
     2521 tatcccgaaa tgcacacgcc catgccgggc cgcaccaatg atctgtggga taccgtcctg
     2581 ctggcggccg cactcgggct ggccgcgtgg ctcgtgttga aacggcgatc ccggccggcg
     2641 gtgtttgggc tcacgctttg ttcgctggcc tatttcggat tctggcggca gggctgtatc
     2701 tgccctgtcg ggtcgatgca aaacgtcgcg gagggcatcc tgggcgtgga agtggtttcc
     2761 atcgccgtgc tgagtttctt cctgctgccg ctgatattcg ccctgttttt cggccgggta
     2821 ttctgcgcgg cggtgtgtcc gctgggggcg atacaggaag tagtagctgt gcgtccggtc
     2881 atgctgcccc gcgcggtgga ccgggttctg ggcttcgtac cttatatcta tctcgccctc
     2941 acgattctgg ccgtttcgac cgggtcgggg tatctgatct gccggtacga ccccttcgtg
     3001 ggatttttcc gattgggggc gagtctgaac atgttcctgg cgggcggggt gctgttgctg
     3061 accgggctgt tcatcggccg gccgtattgc cggtttttgt gcccgtacgg cgtgcttctg
     3121 ggctggatga gccggttctc aaaatggcac gcgagcatta cgccgcggga gtgcatccag
     3181 tgccgcttat gcgaaaacgc gtgcccttac ggcgcgatca atatccccac gccggataag
     3241 acgcccctca ccgcaaagga aggcgcgcgc aaactgggcg tgctgcttgc cctgaccccg
     3301 attctcatcg caatcggcgc gctgaccggg ctcttctcgt atccgatgct cgcgcgaatc
     3361 cacccgacgg ttcagcttgc gtcgcgtatc gccggcgagg aggcgggccg gtatcaaggc
     3421 atgaccattg agagcgaggc gtttcgagcc ggagttaaga cgcaggatga actctatgcg
     3481 gaggcgcacg cgattcgcgc ggatttcaag tggaaaaccg cactgtgtgg cgcatttctg
     3541 ggtctggtga tcgctgtgac gctggcgagc ctgtcgatgg cgggctgccg cgatgattat
     3601 gaaaccgacc ggcgggcctg cgtcagttgt gcgcgatgtt tcccgtactg ccccgtggag
     3661 aaggatgaag ttgctgttgc agaacaaaag acctgaattt gtgccgagcc aggcgaaatg
     3721 gcgcatcgcg tcggccgttg cggcggttgc ggggcttttc accctcgttc tggcctcgtt
     3781 gctcatcatt aattaccttc agattcaggc gacggatccg ctggacaacc cagagctgtt
     3841 gaaattacgc gaacaactgg ccgcttcttc cgggaacaac gaagcgctgg ttgaacagat
     3901 tcgcacgctg gatctcctcg tacgcaaagc gtttttcacc agtcaggcgc agttgcagct
     3961 gggcggccgt ctcttgctgg cgggggccat cgtgtttctg gtggcgttcc gcctggccgc
     4021 acggtggaac cccgtgctgc catcccccgt gggcactccc gataccatga agtattggcg
     4081 gattcttgcg cagtcccggg aattgatcac cggagtggcc gtcgtgctcg tgctggtgac
     4141 gctggccgcg gcctatgtga ccccctttga catccctgcg ccagacttgg aaaaacaagc
     4201 cccggaaggc gcggccgaag cggagacggt gtctgatgct gaggcagtag attttcctac
     4261 ctgggaggag atgctcgccc aatggccatc gttccgcggg ccgggtggct atggcgtggc
     4321 ccatttcacc acggcgcccg tgaactggaa ccttgaggcg ggcgagggca tccggtgggc
     4381 tgtggatgcg ccgcagggcg gcttcaactc gccggtggtg tgggataacc gcctctttct
     4441 cagcggagca agcaaagaac tgcgcgaagt ctactgttac gacacggaga cgggagagct
     4501 ggtgtggcgt caggccgtgc ccgcatttcc cggcacgccc gagacgctgc cccgggtctc
     4561 ccaggatacc ggctatgccg cgccgaccat ggccctgctc ggagaccgcg cctgcgtgat
     4621 ctacgccacg ggcgatatcg cgtgttttga ttttgacggc ggcctgatct ggggccgcaa
     4681 cctcggcgtg ccggagaatc actacggcca cgcatcgtcg ctgattgcat atgccggcag
     4741 ggtgtatgtc cagctcgacg acagcaaaca gccccgcctg ctcgccctcg atgcagcgac
     4801 gggcgaagac gtttggattg cagagcgaaa gaagatctcc tgggcatcgc cggcgtgcgc
     4861 gccgactccg ttcgggtttc aactgctcct ggcttccgaa tacgatgtgg atggctatga
     4921 cccggcgacg ggctcgttat tgtggacgca gaaggggctg gacggcgagg ttgcgccttc
     4981 ccccacgatc tccggcgatg tagtgttcgt ggggaacgaa tatgccatgg cgacagccgt
     5041 ccgtttgacg agctcaaacg agggcgtgca atccgaaatc atctggcagt gggaggacag
     5101 tctgccggaa gtggcaagtc ccacgtcctg tgacgagtgt ttctacatcg cgacatcgct
     5161 gggtgaaata atctgcctgg acaaggacac gggcgaggaa gcctggcttc acgaattcga
     5221 tcaggggttt tattcgtctc cgatccgggt gggtgaccgc atctacgccg ccgatctgaa
     5281 aggaacgatg cagatattcc gcgcgggccg ggaatttgaa ccgatcgccg cgctggattt
     5341 gggcgaaccc gtatacgcga caccggcata cctcgacggg cggatatatg tccgtactga
     5401 gagccgcctc ctgtgtattg aagacaatgc tgcacactga attttgcata gacgacgtcg
     5461 atcgcctcgt agcggaaatc ggacatggca gggaggccgt gatcccgatc cttcatgcgc
     5521 tgcaggaccg gtaccgctac cttcccgacg ccgccctcgc ccgggtctgt gagattaccg
     5581 agatcacgcc cgccactatc gagggcgtcg cgacgttttt cacccggttc cggcggcggc
     5641 cgatgggcga acacgtcatt cgagtctgcg acggcacggc gtgccatgtg aaaggggcgc
     5701 ccgcggtatt cgacgcgttc gccaaggcgc ttgacctgag cccgggtgaa gacacggaca
     5761 aacagggcca gttcaccgtg cagcgcgtgg cctgcctcgg gtgctgcacg ctcgcgcccg
     5821 cggtgcagat cgaccacgtt acctacggcc acgtcgagtc ggaaaaggtc tcttatctgc
     5881 tggaagactt tctcgcaaac gaagccaacg gaaaagcggc tccgggcagg cgccaggccg
     5941 aatccgcgcc cgcggccggc gagatccggg tcggcctggg ttcctgttgc attgcgggcg
     6001 gcagcgagaa ggtccgggtc gcgctggaag acgcgctgca gaccatcgca gggtcggttc
     6061 gcatcaaacg ggtgagttgc gtgggcatgt gccaccagac tcctttgctc gaagtacatt
     6121 tgccgggccg tccgcccagc ctgtatgcga aggtgcaggc cgaggatgtg ggcgagatcg
     6181 tgcgccggca cttcgcgccg cgtaaacaca gcgttcgcat ccgcacagac atcggccact
     6241 ggctgagacg tctctatacg ggcgagcggc ggactgtgct cgagcggcac gggatcgatg
     6301 tgcgagaccg tcccgttgcg gcttttttga gcaagcaaca gcggctggcc acggagtatt
     6361 gcggcgaaat cgatccggtt gacctcagtg aatacctgcg gttgcgggga tttgaagcgc
     6421 ttgcccgctg cctgccgcag cctgcacctg ccggagacga ctcgattgcg catctgactc
     6481 cggacgccat catcgcggaa ctccgtgaga gcggtcttcg cggcagaggc ggagcggggt
     6541 ttccaaccgc gcagaaatgg gagatcgtgc gcgcggcggg ggatatgccc aaatacgtga
     6601 tctgcaacgg cgatgaaggg gaccccggcg cgttcatgga tcgcatgatc ctggagtcgt
     6661 atccgttccg ggtgattgag ggaatgatca ttgcgtcggt tgcggtcggc gcgcacgagg
     6721 gatttctgta catccgcgcg gagtatccgc tggcgctggc ccgtatcgag gaagcgctgc
     6781 atatcgctga gcaggcggga tatcttggcg acaatatcct gggcagcggc cacgccttcc
     6841 ggatgcaaat catcgaaggt gcgggcgcgt ttgtctgcgg cgaggaaacg gccttgctcg
     6901 cagccatgga agggcgccgt gcgacgccgc acatacggcc gccctacccg gcggtggccg
     6961 gcttgcacgg gcacccaacc ctggtgaaca acacggagac tttcgcgctg gtgccatgga
     7021 tcatccgcca cggggcggcc gcattcgcgg ccttcggcac cgcgaagagc cggggcacca
     7081 aagtcttctc cctggcgggc aaagtgaacc ggggcggcct catcgaagtg ccgatgggca
     7141 ccacaattcg cgagatcgtg gaagacatcg gcggcggggt tcagggcgga aaacagttta
     7201 aggccgtgca ggtgggtggc ccgtctggcg gatgcatacc ccgcgcgctg gccgataccc
     7261 ccgtggattt tgaggctctg gcgacggccg gcgccatgat gggatccggc gggctggttg
     7321 tccttgacga gaccgattgc atggtcgaca tgacgcggta ttttctgtcg ttcacccagc
     7381 tggaatcctg cggaaaatgc acgccgtgcc gggtgggcac gaagcgcatg ctcgagatcg
     7441 tcgagaatct ctgcgaagac aaggccgaac cgaaggatct ggcggaactg gagacgctcg
     7501 cgcgcgtcgt caaagacaac agtctgtgcg ggttgggcaa gaccgccccg aaccccgtcc
     7561 tgaccgcgat gcagtatttt cgggacgaat tcgaggccca cgcgcaaggg cggtgcccag
     7621 ccggcaaatg caaaagcctg atcacctatt ccattaccga aaattgcatc ggatgcacca
     7681 aatgcgccgt gaactgtcct tcaggcgcca tcgagttccg gccctaccag cagcatgaaa
     7741 tcgacccggc cctgtgcacc cgctgcaacg gctgttatg
//
LOCUS       DAOPBQ010000103         7771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8528_length_7771_cov_4.519051, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000103 DAOPBQ010000000
VERSION     DAOPBQ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7771
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8528_length_7771_cov_4.519051"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="PKW60_04870"
     CDS             complement(<1..1422)
                     /locus_tag="PKW60_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPJ98795.1"
                     /translation="MARKKRENTDRLPLLPLKDVVVFPQMVLPLLVGRPASIAAVEES
                     LSSGRPLFLCSQVDPDVESPGIKDLHTVGVAGNILQTLRMPDASMKVVIEGMARGRVL
                     KLARKDGYVEVEVERVEIDQIEAGEETLGRMRMALRQFEDYAHASQRVSPEIVGSLHG
                     IAEPAALADLICAYLPLSATERQELLEELDPALRLEHISSLLMRENDLMDIERRVRDR
                     VRDQMERSQREYYLHEQLKAIQQELGTREEGADEFADLRRQIDKAHMPKEVRQKALRE
                     YSRFERMPSMSPESGVIRTYLEWLVDVPWSRRSRDIIDIAEARRVLDEDHYGLTKVKE
                     RILEFLAVRKLNKKSKGPILCLVGPPGVGKTSLGKSIARAMKRKFVRVSLGGIRDEAE
                     IRGHRRTYVGALPGRIIQGLVRGGVKNPVFMLDEIDKMSMDFRGDPSSALLEVLDPEQ
                     NHAFSDHYLEVDVDLQEVFFVTTA"
     gene            complement(1623..2900)
                     /gene="clpX"
                     /locus_tag="PKW60_04875"
     CDS             complement(1623..2900)
                     /gene="clpX"
                     /locus_tag="PKW60_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPJ98796.1"
                     /translation="MAPHRTRGDVPSCSFCGKRHDEVSKLIAGPEVNICDECVQLCSE
                     IVAEDRARKGVSRGLKVPRPDEIFSYLDQYAIGQERAKRILSVAVHNHYKRILSGGEI
                     DGVELHKSNVLLIGPSGCGKTLLAQTLAKMLDVPFAVVDATTLTEAGYVGDDVENVIL
                     KLLQAADFDSARAETGIVYIDEIDKIARKGDSPSITRDVSGEGVQQALLKLLEGSVAS
                     VPPQGGRKHPQQECIQVDTTNILFICGGAFNALEKIIERRTRQNVMGFNANVQSRRER
                     SVGELLELVEPEDLIKYGMIPEFSGRIPVIATLHELNKEDLRRILVEPKNAIVRQYQK
                     LFEMENVKLRFSEETLDAVADYAIEKDTGARGLRSILEQAMLDVMFEIPSRKDVRECI
                     ITPGVILNKEEPLLVYEHEAEGRPKKGTSSASA"
     gene            complement(3130..3750)
                     /gene="clpP"
                     /locus_tag="PKW60_04880"
     CDS             complement(3130..3750)
                     /gene="clpP"
                     /locus_tag="PKW60_04880"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPJ98797.1"
                     /translation="MNEYLRNPLDLRAVTIYEDTSRGERPYDIYSKMLKERIVFLGFP
                     IDDHVANYVIAQLLFLESEDPDKDIQLYINTPGGSVTAGLAIYDTMQYIKPDVSCICL
                     GQAASMGALLMAAGTQGKRFALPYSRFLLHQPMGGARGQASDIDIQAREIVRVSEMID
                     EILANHTGQPIETIRRDSDRDFFMDAQEAKDYGLVDEILRRGAVKS"
     gene            complement(3743..5218)
                     /gene="tig"
                     /locus_tag="PKW60_04885"
     CDS             complement(3743..5218)
                     /gene="tig"
                     /locus_tag="PKW60_04885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPJ98798.1"
                     /translation="MAEETKKHDDEKQSELDESTDAAAEETAVVPAAQDDAEDEEDFV
                     EDPQFEVDYKGDCAYEVKVSIPPANRSHKSAEMFDELKKEAEVPGFRPGRAPRKLIER
                     KFGKIVKGEVDAKLVSAAFHRLIKVEELHPLGIPEIDGLEEKKERADDEPLDFTFKFE
                     VAPRVELGDYKGIAVERPVVKVADKDVQEAVDGLRSRYAVFETLQEGEAAEGDQVIID
                     FKGTVDGAEFAGGSANGYPYILGTKRFFPEFEAALLGSSPGAELSCTVTLPENTPNEE
                     LRGKKADFTIVVKELKRRELPELNDDFAKQAGYESLDDMKAKIAERMQEGASEQSNRI
                     AENRALESVINASTFELPKSLLEEMADSIYREQVSRLRHSGHPMADIEAREDELRKRA
                     REEAEADVKASTVLNKIGDVENIVISDDDYEQEVASIARNAGVQSDAVASFLEEGDRR
                     SRTEGRLYRAKALAIIMANAKVQDKEVKEDELKAQENANDE"
     gene            complement(5281..5365)
                     /locus_tag="PKW60_04890"
     tRNA            complement(5281..5365)
                     /locus_tag="PKW60_04890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5329..5331),aa:Leu,seq:tag)
     gene            5743..7035
                     /locus_tag="PKW60_04895"
     CDS             5743..7035
                     /locus_tag="PKW60_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPJ98799.1"
                     /translation="MKDSELNIGLMQLVFPMMKTVDMMSPALASHHLDVAYLALRISE
                     QAGLPADDIQTIVLAGLMHDIGAFSLDERLDILEFEYHQPERHCNAGYELLRQFEPFA
                     RAAGIIRFHHLRWNNGEGSQRDGVAVPLGSHILHLADRIAVLVPKNIHALSKVAEICK
                     KIQELRGSWFHPDMTDAALALARHDHIWLDVVSHAVERPLMECLEGSHRLATLDELLS
                     FGELVCRLIDFRSPFTATHTSGLAAAASALAERLEFEEPQQKLLKLAAYFHDLGKLAV
                     PTEVLEKAGQFTDDEWHTMRAHVYYTFRALSSLPALDKLTSWSALHQERLDGSGYPFG
                     YTADRLPLGARVLAVADVFVAITEDRPYRNGMPEHEARTVLADMAARRQLDPDIVRAL
                     FDDFGAINTIRAEAQAKARAAFQRFRENLSGCTKDDSV"
     gene            complement(7093..>7771)
                     /locus_tag="PKW60_04900"
     CDS             complement(7093..>7771)
                     /locus_tag="PKW60_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98800.1"
                     /translation="NEIYRVVQESDDQGGADMWGDATFRGNVYHYNYWHHIGNQTNPA
                     DAPDCGQAGIRLDDAISGTRICGNVFHRTSSGKAGFGGVQIHGGKDNLIDNNVFVECM
                     AAVSFSAWGDARWRDFVGNAMESPEIDSELYVQRYPELARLLEDHDINILARNLALNC
                     GEFIRRDSKRGILLDNVDTSTEIALTGDGRLDLQALTALMREHGLAPIPFEEIGLYES
                     DTRSQTP"
BASE COUNT         1472 a         2478 c         2131 g         1690 t
ORIGIN      
        1 ggcggtcgtg acaaagaaga cttcctgcaa atccacgtct acttcgagat agtggtcgct
       61 gaaggcatgg ttctgctcgg gatccagcac ttcgagcagc gcggacgacg gatcgccgcg
      121 aaaatccatg ctcattttgt cgatttcgtc gagcatgaac accgggttct tgacgccgcc
      181 gcgaaccagg ccctggatga tgcggcccgg cagcgcgccc acgtaggtgc ggcggtgtcc
      241 ccggatctcc gcttcatcgc gaatgccgcc cagcgacacg cggacgaatt ttcgtttcat
      301 ggcgcgcgcg atcgatttgc cgagcgacgt cttccctacg cccggaggcc ccacgagaca
      361 gaggatcggc cccttggact tcttgttcag cttccgtacc gccagaaact ccaggatgcg
      421 ttccttgacc ttcgtcagac cgtaatggtc ttcgtcgagc acgcggcggg cttcggcgat
      481 gtcgataata tcgcggctcc gccggctcca tggcacgtcc accagccatt ccaggtaggt
      541 gcgaataacc ccgctttcgg ggctcatgga cggcatccgc tcaaagcggc tgtactcccg
      601 cagcgccttc tgacgcactt ccttgggcat atgcgcttta tcaatctgcc tgcggagatc
      661 ggcgaattca tcggcgcctt cctcccgggt gcccagttcc tgctggatcg ccttcagctg
      721 ctcgtgcaga tagtattccc gctggctgcg ctccatctgg tcccgtacac ggtcgcgcac
      781 ccggcgctca atatccatca agtcgttttc acgcatcaac aggcttgaaa tgtgttccag
      841 gcgcagcgcg ggatccagtt cttcgagcaa ttcctgccgt tccgtcgcgc tcagcggcag
      901 gtaggcgcag atcaggtcgg ccagggcggc gggttcggcg atgccatgaa gtgaacccac
      961 aatttccggc gacacacgct ggctggcatg cgcgtagtct tcaaactgcc gcaacgccat
     1021 gcgcatgcgc cccagggtct cttcgcccgc ttcgatctgg tcgatttcaa cccgctcgac
     1081 ctccacctcc acatagccgt cctttcgggc gagtttcagc acgcggccgc gcgccatgcc
     1141 ttcgatgacc actttcatgg aggcgtcggg cattcggagc gtctggagga tatttccggc
     1201 cacgcccacc gtgtgcagat ctttgatccc aggcgattcc acatccggat ccacctgcga
     1261 gcacaagaat agcggccggc ccgaggacag gctctcttcg accgccgcga tggacgcggg
     1321 gcgcccaacc agcagcggaa gaaccatttg cggaaatacc accacatctt tcagcggcaa
     1381 caacggcaag cggtccgtgt tttctcgttt cttgcgtgcc atacgtgctt ctttcgtact
     1441 cctgtgcggc gaggcgccct cacgatttca acgcaatgcc ggagggaatg tcaattcggc
     1501 cgccgcgggt ccgtccatgc gcgcacaaag accggccgga gggacagggc gcccctccgg
     1561 cctccaaagg tgatcctctc gcgcgaaaac tatgatgagg tccggcgtct ggcgggcggg
     1621 cctcaggccg aagccgatga cgtccctttc ttgggacgcc cctcggcttc gtgttcatac
     1681 accaaaaggg gttcctcttt gttgagaata acacccggcg tgatgatgca ttcgcgaaca
     1741 tccttccggg acgggatttc aaacatcaca tcgagcatcg cctgctcgag aatactccgc
     1801 aatccgcgcg cgccggtgtc tttttctatc gcgtaatccg ccacggcgtc cagcgtctct
     1861 tcgctgaaac ggagcttcac attttccatc tcaaacagct tctgatactg tctgacgatc
     1921 gcatttttcg gctcgaccag gatgcggcgc agatcttctt tgttcaattc atgcagggtc
     1981 gcaatcacgg gaatgcggcc cgaaaactcg gggatcatgc cgtatttgat caggtcctcg
     2041 ggctcgacca gttcaagcag ctcaccgacg ctccgttcgc gccgcgactg cacattggca
     2101 ttgaacccca tgacattctg acgtgtgcgc cgttcgatga tcttctcgag cgcattgaag
     2161 gccccgccgc agataaacag gatgttcgtt gtatcgacct gaatacactc ctgttgcggg
     2221 tgtttccgtc cgccctgggg aggaacgctt gccaccgagc cttccaggag cttcagcagc
     2281 gcctgctgca ccccttcgcc ggagacatcc cgcgtgatcg aggggctgtc tcctttgcgg
     2341 gcgattttgt cgatctcgtc gatatagaca atgccggtct ctgcgcgggc cgaatcaaaa
     2401 tccgccgcct gcaacagctt caaaatgaca ttctcgacgt catcccccac gtacccggcc
     2461 tcggtcaggg tcgtggcgtc caccacggca aacgggacat ccagcatctt cgcaagtgtc
     2521 tgcgccagaa gcgttttccc gcaacccgac gggccaatca acagcacatt cgacttgtga
     2581 agctcgaccc cgtcaatctc gccacccgac agaatccgct tataatggtt gtggaccgcg
     2641 accgacagaa tccgttttgc acgctcctgc ccaatcgcat attggtccag gtaactgaaa
     2701 atttcgtcgg ggcgggggac tttcagtccg cgggagacgc ctttgcgggc ccggtcctcg
     2761 gcgacgattt cggagcagag ctggacacac tcatcgcaga tgttaacttc aggtccggca
     2821 atgagtttgc ttacttcgtc gtggcgcttg ccacaaaagg agcagctggg gacatcacct
     2881 cgcgtgcgat gtggagccat aaatacctcc cttggagatg agactgtcga cgatggttat
     2941 agcattctcg cgccgttttt gtcaacagtt ttctcagcca aggtcttcga tactgcgcct
     3001 cgcctcttca attatgcgga aaatttgttt tttccgcaac tttcccccgg tatacctcgc
     3061 atactcaccg cagcccatac ggccggggga ggcacagtct atgttttctg cgcatactgc
     3121 gcatgcaact caggacttga cagcgccgcg gcggaggatt tcgtcgacaa ggccgtagtc
     3181 ttttgcctcc tgggcgtcca tgaagaaatc gcggtcgctg tcgcgccgga tagtctcaat
     3241 gggctggccg gtatggttgg ccaggatctc atcaatcatc tcgctcacgc ggacgatttc
     3301 acgggcctgg atgtcgatgt cggacgcttg cccgcgcgcg ccgcccatcg gctgatgcag
     3361 gaggaatcgg gaatacggca gggcgaaacg ctttccctgc gtgccggccg ccataagcag
     3421 ggcacccatg ctggccgcct ggcccaggca gatgcatgac acatcgggct taatgtattg
     3481 catggtgtcg tagattgcca gaccggcggt tacactgccg ccgggcgtat tgatgtagag
     3541 ctgaatgtct ttgtccgggt cttccgactc cagaaacaac aattgagcga tgacgtaatt
     3601 ggcgacgtgg tcgtcgatgg gaaacccgag gaaaacgatg cgttctttga gcatctttga
     3661 atagatgtcg tagggccgct ctccgcggct cgtatcttca tagatcgtaa ccgcacgcaa
     3721 atccagaggg tttcggaggt attcattcat cgttcgcgtt ttcctgggcc ttcagctcgt
     3781 cttccttgac ttctttgtcc tgcactttgg cgttggccat gatgatcgcc aaggcctttg
     3841 cacggtacag gcggccttcc gtgcggctgc ggcggtcgcc ttcttccagg aaactggcaa
     3901 ccgcatcgga ttggacaccc gcgttccggg caatggatgc gacctcctgt tcgtaatcgt
     3961 cgtcggaaat cacaatattt tcgacatcgc cgatcttgtt caggactgtg ctcgccttga
     4021 catccgcttc ggcctcttcg cgcgcccgtt tgcgcagttc gtcttcgcgg gcttcgatat
     4081 ccgccatcgg gtggccactg tggcgcaggc gagaaacctg ctcgcggtag atactgtcgg
     4141 ccatctcttc gagcaggctc ttgggcaatt caaatgtact ggcgttgatc acgctttcga
     4201 gcgcacggtt ttcagcaatc cggttactct gctcggacgc gccttcctgc atccgctccg
     4261 cgatctttgc cttcatatca tcgaggcttt catagcccgc ctgcttcgcg aagtcgtcgt
     4321 taagctcggg cagttcgcgg cgcttgagct ccttcaccac aatagtaaaa tccgctttct
     4381 tgccgcgaag ttcctcgttt ggcgtatttt caggcaacgt cactgtgcag gaaagttccg
     4441 cacccgggga actgccgagc aacgccgctt cgaattccgg gaagaaccgt ttggtgccca
     4501 gaatataggg atatccattc gctgatccgc ccgcaaattc cgcgccgtcg accgtgcctt
     4561 tgaaatcgat aatgacctgg tcgccctctg ccgcctcgcc ctcctggagc gtctcaaaca
     4621 cggcgtagcg cgaacgcagc ccatccacgg cttcctgcac atctttatcg gcgaccttga
     4681 ccacgggccg ttctaccgcg atgcccttat agtcgcccag ctcgacacgc ggcgccacct
     4741 caaatttgaa cgtgaagtcc agaggctcgt catctgcgcg ctctttcttc tcttccaggc
     4801 cgtctatctc agggatgccc agaggatgca gctcttccac tttgatcagg cgatggaacg
     4861 ccgcactgac gagtttcgca tcgacctcgc ctttcacgat cttgccgaac tttcgttcga
     4921 tgagtttccg gggcgcccgg ccaggacgga accccggcac ttcggcctcc ttcttcaact
     4981 catcaaacat ctcggccgat ttgtggctcc ggttcgcggg aggaatggag accttgactt
     5041 cataggcaca atcgcctttg taatcgacct cgaactgcgg gtcttcaacg aagtcttcct
     5101 cgtcctccgc atcatcctgc gcagcgggca ctacagcggt ttcttccgcg gcggcgtcgg
     5161 tgctctcatc cagttcagac tgtttttcgt cgtcgtgttt ttttgtttct tctgccatgg
     5221 ggtcttctct acctctgtcg acattgccac ttggcctaca cggcgtttac ttacggcgat
     5281 tggtgggcga ggcgggactc gaacccgcac accttgcggc actggatcct aagtccagcg
     5341 cgtctgccaa ttccgccact cgccccggag aacaccctct ccggagatgc cattccatgc
     5401 ccgggagaca ttctaacacg gcattccgcc tgagatatgg cgcccgaccc gggatagaaa
     5461 aagatgggcg gtgcggccaa ggcctcggcg attcccgaag cggcgtccgt gcccacgcat
     5521 caaatccaaa cgcccatgct accacaataa aatcaagaat ttcaacacgt tgtcgcatca
     5581 atcccacgcc gctcaggccg ccggagcatc gtcagcccgg ccgggtgcgg catctcccgg
     5641 ccacggcgaa ggctcgcccc agaaaagtgc attgcgggcc agacctgtag taaggtaatc
     5701 gttagaaacc ggagcggcac ggccaacgca gaggactatc gcatgaaaga ctctgagctc
     5761 aacatcggtt tgatgcagct ggtcttcccg atgatgaaga cggtcgacat gatgagtccc
     5821 gccctggcaa gccatcatct ggatgtggcc tacctcgcct tgcgcatcag tgagcaggcg
     5881 ggcctgcccg cggacgacat ccagaccatc gtcctcgccg gactgatgca cgatattggc
     5941 gcgttttccc ttgacgagcg cctggacatc cttgagtttg aatatcacca gccggaacgc
     6001 cattgcaacg cgggctatga actgctccgg cagttcgagc cgttcgcccg ggccgccggc
     6061 atcatccggt ttcatcacct ccgctggaac aacggagaag gcagccagcg cgacggcgta
     6121 gccgtgcccc tgggcagcca catcctccat ctggccgacc gcatcgcggt gctggtgcct
     6181 aaaaatatcc acgccttgag caaagtcgcc gagatctgca agaaaattca ggaacttcgc
     6241 gggtcgtggt ttcaccccga tatgaccgat gcggcgctcg cactggccag gcacgaccac
     6301 atctggctcg acgtcgtctc ccacgcggtc gaacggccgc tgatggaatg ccttgaagga
     6361 tcgcaccgtt tggccaccct ggatgaactc ctctcatttg gagaactcgt gtgccggctc
     6421 atcgacttcc gcagcccgtt taccgcaacc cataccagcg gattggcagc cgccgcgtcg
     6481 gcactcgcgg agcgcctgga atttgaagag ccgcaacaga agctcttgaa gctcgccgct
     6541 tacttccacg acctcggcaa actggccgtc ccgaccgagg ttctcgaaaa agcgggccag
     6601 ttcaccgacg acgaatggca cacgatgcga gcgcacgtgt actacacgtt tcgcgcgttg
     6661 tcatcccttc ccgccctgga caagctcacg tcctggagcg ccctccacca ggaacgcctc
     6721 gacgggagcg gctatccgtt cgggtatacc gccgaccggc tccctctcgg cgcgcgggtg
     6781 ctggctgtcg cagacgtctt tgtcgccatc accgaagacc gcccgtaccg caacggcatg
     6841 cccgaacatg aagcgcgaac cgtgctcgct gacatggcag cacggcgcca gctcgatcct
     6901 gacatcgtgc gcgccttatt cgacgacttc ggcgctatca atactatccg cgccgaagcc
     6961 caggccaaag cacgcgccgc atttcagcgg tttcgggaaa atctgtcggg ctgcactaaa
     7021 gacgacagtg tgtagacccg ctggatacgc gcgcccggtc cgcgccatca aagcccgcgc
     7081 tcgtgacctg cgttacggag tctgcgaccg ggtatcgctc tcgtagagcc cgatttcctc
     7141 gaatggtatc ggcgcgaggc catgttcccg catgagcgcc gtgagagcct gcagatccag
     7201 acgcccgtcg ccggtcagag cgatctcggt tgacgtatcg acattatcga gcaaaatccc
     7261 gcgtttgctg tcgcggcgaa tgaattcgcc gcagttcagc gcgagattac gcgcaagaat
     7321 attgatatcg tggtcttcta gaaggcgcgc gagttcagga taccgctgca cgtagagttc
     7381 cgaatctatt tccggcgatt ccatggcatt gccgacgaag tcccgccagc gcgcatcccc
     7441 ccacgccgaa aaactgacgg ccgccatgca ttccacaaac acgttgttgt cgataagatt
     7501 gtctttgcct ccgtggatct gcacgccgcc gaacccggcc ttccccgacg aagtccggtg
     7561 aaacacgttg ccgcaaatgc gcgtgcccga tatcgcgtca tcgaggcgga tgcccgcctg
     7621 cccgcaatcg ggggcatccg ccgggttggt ctggtttcca atatggtgcc agtagttgta
     7681 atggtagaca ttgccgcgga acgttgcatc gccccacata tcggcgccgc cttgatcgtc
     7741 ggactcctga accacacgat agatctcgtt c
//
LOCUS       DAOPBQ010000104         7734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8575_length_7734_cov_4.365021, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000104 DAOPBQ010000000
VERSION     DAOPBQ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8575_length_7734_cov_4.365021"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..2581)
                     /gene="gyrA"
                     /locus_tag="PKW60_04905"
     CDS             complement(44..2581)
                     /gene="gyrA"
                     /locus_tag="PKW60_04905"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPJ98801.1"
                     /translation="MYTRNEKIVPIAIENEMKNSFMDYSMSVIVSRALPDVRDGLKPV
                     HRRILFTMHELGLVHNRGFRKCAAVVGDTMGKYHPHGDSAIYDALVRLAQPWNMRYAL
                     IQGQGNFGSVDGDSPAAMRYTEARMAAICAEMLGDIEKETVNTTPNYDGRLQEPQVLP
                     SAIPNLLVNGSYGIAVGMATSCPPHNLTEVCDAVVHLIDHPEATTKDLLQFVKGPDFP
                     TGGIICGREGIKKAYLSGRGQVRVRAHVNVETNEKTGKESLVVTEIPYQVNKARLIEN
                     IANLVRSKKVEGISDLRDESDKDGMRVVIEIRKGDEPKVILNQLFQFTQLQDTASIHL
                     LALVNGSPRVLSLREMVHYFIEHRAEIVERRTRFDLAEAEKRAHILEGLLKAIDHIDE
                     VIAIIRASADSESASRALIERFTFSEVQANAILAMRLRRLTGLEREELEKEYADLLKE
                     IERFRNILSSERTIKAEVRKETLKVKEKYGDARRTAIVGEVEEIGLEDMIADESMVVT
                     VSNAGYIKRLPVSTYRKQRRGGKGVTGMGTKEEDSVRDLFIATTHQYMLFFSNAGRVY
                     WRKVHELPKAGRAARGRAIVNLLNLSGGETITACLPVGDLKEEGKNIFMVTRNGTVKK
                     TLLKAFSNPRATGIIAIGLDPGDQLIDVQITSGEDNILLATAHGKAIRFPEKDVRTMG
                     RTARGVVGIRLEEGDFVIGTSIAEDESTVLTVTEHGYGKRTKVGEYRLQHRGGQGIIN
                     IKTSERNGRVVGMLTVDDYDEIVTVSTDGIVMRCGVKDIRTIGRNTQGVKVMSPSPGA
                     LVSAVAKAVADSKEERITGDPASDLDIPEDDIGENGSEDLEPGGEEE"
     gene            complement(2665..5070)
                     /gene="gyrB"
                     /locus_tag="PKW60_04910"
     CDS             complement(2665..5070)
                     /gene="gyrB"
                     /locus_tag="PKW60_04910"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPJ98802.1"
                     /translation="MAKESYGAGTIQVLEGLQAVRKRPAMYIGDTNVRGLHHLVYEVV
                     DNSIDEALNGSCTQISVTIHIDNSVSVVDDGRGIPVDNHPKYRNKSALEVVMTTLHAG
                     GKFDNSNYKVSGGLHGVGVSCVNALSEWCEVEVKRDGFSHVMSFRQGVPQGKLEKRAR
                     TKSTGTRVTFRPDPEIFEVAEYNADTLLGRLRELAFLNKGLKITFEDERTDDEALKMQ
                     YKGGIIEYVEYLNRNRETLHRKPIYFEAERDRLQCEIALQYTTAYTETVSSFANNINT
                     HEGGTHLSGFRAALTKALNDYARKNNSNKKADMNISGDDAREGLTAIVSVRVPNPQFE
                     GQTKMKLGNSEVQGIVNSMVYEGLQTFFEENPTIANRIIGKTIEAARAREAARKAREL
                     TRRKGALDNSALPGKLADCSEKDPALCELFIVEGDSAVGSAKQGRDRRYQAILPLRGK
                     ILNVEKARADRMLSNAEIRALITAIGVGFGKDEFKTENLRYHKVIIMTDADVDGAHIR
                     TLLLTFFFRQMPELIKRGHLYIAQPPLYQVKRGKKVQYINTEDQMEQFLLENILDEVS
                     VLAANGSPRSSKVTLKTLRKALRAALERENLLSRLNRVYGASREAVEKAMLLPREKWM
                     NPATLSPTEKNDLFGDLEIIDTGQVQGELPQNGDENGKTKPKRQKGQVDIAFMRSHEF
                     SALLSGKEPLSEIGNPPFRVEPIKEGEPFETNDLLSLRDHLLELAKKGLTIQRYKGLG
                     EMNPDQLMETTMNPDSRTVLRVEAEDETAADDMFVTLMGDLVEPRKAFIEKYAAEVRN
                     VDT"
     gene            complement(5190..5552)
                     /locus_tag="PKW60_04915"
     CDS             complement(5190..5552)
                     /locus_tag="PKW60_04915"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPJ98803.1"
                     /translation="MLHSVEARLVTRKKDPSSIQDVLAQLKRETELGRQLELAQIWSR
                     WPEIAGPDLMEHGRPHGIRDNTLYIEADSPVWMHTFAFQKWRLIGRINRMAGHELISD
                     IFLVLSEGEEPADSQDGV"
     gene            complement(5560..6651)
                     /gene="recF"
                     /locus_tag="PKW60_04920"
     CDS             complement(5560..6651)
                     /gene="recF"
                     /locus_tag="PKW60_04920"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPJ98804.1"
                     /translation="MSSRCAMVLRELHAEGFRCLPLLTFAPDPGLNVIFGDNAQGKTS
                     LLEAVLYAATSKSHRTSQETDLVQHGCHGFRIRAAAQRSDRNVVVEATWWRGEKRFKV
                     NGVSQSRVSDILGRLPVVFFSPEDAELVRGTASVRRRFLDMALSQLDLPYLRALQQYR
                     QALRQRNELLKQDQVTGDLLDAWDEQLVRHGTVMVRSRLLFGQQLANFAAPAYDAIAT
                     RERLELVYLPDIRSDEDYAEVLRENRDRDRRYGLTSRGPHRDDFELKVEGRPARQFAS
                     QGQQRSVALALKLAELKLVRERISEYPVLMLDDVLSELDAQRSSRLFNAIPEEAQCLI
                     TTTGMATATVPSGLACTLFQIRNGALTRA"
     gene            complement(6648..7499)
                     /locus_tag="PKW60_04925"
     CDS             complement(6648..7499)
                     /locus_tag="PKW60_04925"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPJ98805.1"
                     /translation="MNTSRDIEFLLEEIITLPSLPGALARIVQLLNDPESSLSDVGKA
                     ITQDPAVALKTLRLANSAAYGLREHVTSVEHAVALLGIKVIHNLVLTATVFDTLSRSA
                     GLLYQHSIVAGLAARAVLRTGRVALALQPDEAFVYGLLHDIGKIILEDYLSDDLMEAE
                     TLSRDRKVPAYQVEREIIGADHAEVGGRLAMNWRLPESIASAILAHHDLSRCENEDSR
                     RLAALLNVADAICIEAGFPSYEGAVAGDTSQSWALLGVESAKIPAIMDDFFASIPEVE
                     ELIEAMS"
     gene            complement(7496..>7734)
                     /locus_tag="PKW60_04930"
     CDS             complement(7496..>7734)
                     /locus_tag="PKW60_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98806.1"
                     /translation="VVGPLTLCDGARGQVVLVGPRNAPGGALVVFQDEEQGPVESLPL
                     ATILERLAPADVVAILCAQERQFGECGLPALDAS"
BASE COUNT         1385 a         2313 c         2314 g         1722 t
ORIGIN      
        1 ttcgcggcac ttggaattcc ggcggctcaa tcaagccgga cgtttactct tcttcgccgc
       61 cgggctcgag gtcttcgctc ccgttttccc cgatatcatc ttctgggatg tccagatcgc
      121 tggccgggtc gccggtgatg cgttcttcct tgctgtcggc gacagccttg gccaccgcgc
      181 tgaccagggc gccggggctc ggcgacatca ccttgacgcc ctgcgtattg cgcccgatgg
      241 tgcggatgtc tttcacgccg cagcgcatga caatcccgtc ggtactcacg gtaacaattt
      301 catcataatc atcgaccgtc aacatgccaa ccacccggcc attgcgttcg gaggttttga
      361 tattgatgat gccctggcca ccgcggtgtt gaagccggta ttcgccgact ttggtgcgtt
      421 taccgtatcc gtgctcggtt acggtgagca ccgtgctttc atcctccgca atcgacgtcc
      481 cgatcacgaa atcgccttcc tccagccgga tcccgacgac gccccgcgcg gtgcgtccca
      541 tggtgcgcac atctttttcg gggaaacgga tggctttccc atgggcggtg gcgagcagga
      601 tattgtcttc accggacgtg atctgcacgt cgatcagctg atcgcccgga tcgaggccaa
      661 tcgcgataat gccggtggcc cgcggattgc tgaacgcttt cagaagggtc ttcttcaccg
      721 tgccgtttcg agtcaccatg aagatgttct tgccttcttc cttgaggtcg cccacgggca
      781 ggcaggcggt gatagtttct ccgccggaca ggttgagcaa attgacgatc gcgcgtccgc
      841 gcgctgcgcg gccggctttc ggcaactcgt gcaccttgcg ccaatagacg cggccggcgt
      901 tactgaaaaa gagcatgtac tggtgcgtcg tggcaatgaa cagatcccgg acggagtcct
      961 cttctttcgt gcccatgcca gtgacgccct tgccgccgcg ccgctgttta cggtaggtgc
     1021 tcacgggcag gcgcttgatg tagcccgcat tcgacaccgt cacgaccatc gattcgtcgg
     1081 cgatcatgtc ttcgaggccg atctcttcca cttcgccgac aattgccgta cgccgcgcat
     1141 cgccgtattt ttctttgact ttcagggtct ctttgcgaac ctcggccttg atggtgcgct
     1201 cgctcgagag gatgtttctg aaccgctcga tttctttcag gagatcagcg tattcctttt
     1261 caagctcttc acgctccagg cccgtgaggc ggcgcaggcg catggcgagg atggcgttgg
     1321 cctggacttc gctgaacgtg aagcgttcga tcaacgcccg gctcgcgctt tcgctgtctg
     1381 ccgaggcccg gatgatggcg atgacttcgt cgatatgatc gatggctttc agcaaacctt
     1441 cgagaatgtg tgcgcgtttt tcggcttcgg ccaggtcaaa ccgcgtgcgc cgttcgacaa
     1501 tttcagcacg gtgctcgatg aagtaatgca ccatttcgcg cagagacagc acgcgcggcg
     1561 aaccattcac cagcgccagc aggtggatgc tggcggtgtc ctgcagctgg gtaaactgaa
     1621 acagttgatt gagaatcact ttgggctcat cgcccttgcg gatctcgatc accacgcgca
     1681 tgccgtcctt atcggactcg tcacgcaagt cggaaatgcc ttcgactttt ttcgagcgca
     1741 ccagattggc gatgttctct atcaggcgcg ccttattgac ctggtaggga atctcggtaa
     1801 caacgagact ctctttaccg gtcttttcgt tggtttcgac attgacgtgc gcccggacgc
     1861 gcacctggcc gcggccggag agataggctt tcttgatgcc ctcgcggccg cagatgatgc
     1921 cgccggtagg gaaatccggc cctttcacaa actggagcag atctttcgta gtggcctctg
     1981 gatggtcaat caaatgcacc acagcgtcgc agacttcggt gagattgtgc ggggggcaac
     2041 tcgtggccat ccccaccgca ataccgtagg agccattcac cagcaggttc ggtatcgccg
     2101 acggcaaaac ctgcggttcc tggaggcgtc cgtcgtagtt gggcgtggta ttgacggtct
     2161 ctttttcgat atcgcccagc atttcggcgc agatggccgc catgcgcgcc tcggtatagc
     2221 gcatggccgc ggggctgtct ccgtcgacgg aaccgaaatt gccttgcccc tggatcagcg
     2281 catagcgcat gttccagggt tgcgccagac gtaccagggc atcgtaaatg gcggagtcgc
     2341 cgtgggggtg gtattttccc atcgtgtcgc cgaccacggc ggcgcatttc cggaagcccc
     2401 ggttgtgcac cagtcccagt tcgtgcatgg tgaacaagat gcggcgatgg accggcttca
     2461 gaccgtcgcg gacgtcgggc agggcgcggc tgacgatgac gctcatggaa taatccatga
     2521 acgagttctt catttcgttt tcgatggcaa tggggacaat tttttcgttg cgtgtataca
     2581 tcgttttcct caactatccg ccaggaacgc cggggtttct cccgccaggc acgtcacggg
     2641 gcaggcagcg ccgggcgcag gcaattacgt atccacattg cgcacttcgg cagcatattt
     2701 ctcgataaag gccttgcgcg gctcaaccag atcccccatc agggtgacga acatgtcatc
     2761 ggccgcggtt tcatcttccg cttcgacccg aagcacggtc cggctgtcgg gattcatggt
     2821 ggtttccatg agctggtcgg gattcatttc gcccaggccc ttgtaccgtt ggattgtcag
     2881 gccttttttg gcgagttcga gcaagtggtc ccggaggctc agcaggtcgt tggtttcgaa
     2941 gggttcgccc tccttgattg gctcaacccg gaacggtgga ttgccgattt cgctcagtgg
     3001 ttccttcccc gacaggagag cggagaactc gtggctgcgc ataaacgcga tgtcgacctg
     3061 ccctttctgg cgtttcggct tcgtcttgcc gttttcatcg ccattctggg gcaactcgcc
     3121 ctgaacctgg cccgtatcga tgatttccag gtcgccaaac aaatcgttct tttccgtggg
     3181 agacagcgtg gccggattca tccatttctc ccggggaagc agcatcgcct tctcgacggc
     3241 ttcgcggctg gcgccataga cgcggttgag gcgcgagagc aggttttcgc gctcgagcgc
     3301 agcacgcagc gctttccgga gcgttttcaa ggtcaccttg ctcgaccggg gactcccgtt
     3361 cgccgctaaa acgctcactt cgtcgagaat gttctcgagc aggaattgtt ccatctggtc
     3421 ctcggtattg atgtactgga ccttcttgcc gcgtttgacc tggtacaaag gcggctgcgc
     3481 aatatagagg tggccgcgct tgatcagttc gggcatctgc cggaagaaga acgtgagcag
     3541 cagcgtccgg atgtgcgcgc cgtcgacgtc cgcgtcggtc atgataatga ccttgtgata
     3601 gcgcaggttt tcggttttga attcgtcctt gccgaatccc acgccgatag cggtaatcag
     3661 cgcccggatt tccgcgttgc tgagcattcg gtcggcccgt gccttctcga cattcaggat
     3721 cttgccgcgc aggggcagga tggcctggta acggcgatca cggccttgtt tcgcagatcc
     3781 tacggcgctg tcgccctcga caatgaacaa ctcgcacaag gcggggtcct tctcggagca
     3841 gtccgccagt tttcccggaa gggcgctatt atcgagcgcg cccttgcggc gggtgagctc
     3901 gcgggccttt cgcgcggcct cgcgtgcccg ggcggcctcg atcgtcttgc cgatgatgcg
     3961 gttggcgatg gtggggtttt cctcgaaaaa ggtctgcaac ccttcataga ccatggagtt
     4021 cacgatgccc tggacttcgc tgttgcccag tttcattttg gtctggcctt caaactgcgg
     4081 attgggcacg cgcacggaga cgatggccgt caagccttcc cgggcatcat cgcccgagat
     4141 gttcatgtcg gcctttttgt tcgaattgtt tttgcgcgca taatcgttga gggccttggt
     4201 cagcgcggcg cggaatccgc tcaggtgggt gccgccttcg tgggtattga tgttgttcgc
     4261 gaagctggac acggtttcgg tataggccgt ggtgtactgc agggcaattt cgcactgcag
     4321 ccggtctcgt tcggcttcga aatagatggg cttgcggtgg agcgtctcgc gattccggtt
     4381 caggtactcc acgtattcaa tgatgccgcc cttgtactgc atcttgaggg cttcatcatc
     4441 ggtgcgctcg tcttcaaagg tgattttcaa gcctttgttc agaaaggcca gctcgcgcag
     4501 gcggcccagg agcgtgtcgg cattgtattc cgccacctcg aagatttccg gatcgggccg
     4561 gaaggtcacc cgcgttcccg tagacttggt tcgcgcccgc ttctccagct tcccctgggg
     4621 cacgccctgc cggaagctca tcacatgtga aaacccgtcg cgcttgacct ccacttcgca
     4681 ccattccgac agcgcgttta cacacgaaac acccacgccg tggagcccgc cggatacctt
     4741 gtagttcgag ttgtcgaatt ttcccccggc gtgcagagtc gtcattacga cttccagagc
     4801 ggatttattg cggtacttgg ggtgattgtc taccggaatg ccgcgtccgt catcgacaac
     4861 gctcacgctg ttgtctatgt ggatcgtcac actgatctgg gtacaactgc cgttcagcgc
     4921 ctcatcgatg ctgttgtcga ccacctcata aaccaggtga tgcaacccgc ggacgttggt
     4981 atctccgata tacatcgcgg gccgttttcg gaccgcttgc agtccttcca gcacctgaat
     5041 ggttccagcg ccgtaactct ctttcgccat tgaacttacc tcacaaattg ggaacatggc
     5101 cggaggccgg aatttcgtcg cggtattcta tcaaaacagg accgaaaaat caaagaaata
     5161 cgcaacattt tgcgtttgaa ggccggcgtc tacacgccat cttgtgagtc cgccggttcc
     5221 tcgccttcgg agagcacaag aaagatgtcg gagatgagct catggccggc cattcggttg
     5281 atccggccga tgaggcgcca cttctggaag gcgaaggtgt gcatccacac ggggctgtcg
     5341 gcttcgatgt agagcgtgtt gtcgcgaatg ccgtggggcc ggccgtgctc catgagatcg
     5401 gggccggcga tttcgggcca gcgcgaccag atctgggcca gttcgagctg gcgtccgagc
     5461 tcggtttcgc gcttgagctg ggccaggacg tcctggatgc tggagggatc tttcttgcgc
     5521 gtcaccagcc tggcctcgac ggaatgaagc acgtccgggt cacgcccgcg tcaaggcccc
     5581 gttccgtatc tggaacagcg tgcacgcgag cccggaaggc acggtggccg tggccatgcc
     5641 ggtcgtggta atcagacact gcgcttcctc cggaatcgcg ttgaacaaac ggcttgaccg
     5701 ctgagcatcc agttcagaca gcacatcatc aagcatcaat acaggatact cggaaattcg
     5761 ctcgcgtacc aatttcaatt cagcgagctt gagggcgagc gcgacgctgc gctgctggcc
     5821 ctgcgacgca aactggcgtg cgggccggcc ctcgaccttg agctcgaaat catcgcgatg
     5881 ggggccgcgg ctggtcaacc cgtaccggcg atcgcggtcg cggttttcgc gcagcacctc
     5941 ggcgtagtcc tcgtcgcttc ggatgtcagg gagatagacc agttcgagcc tctcccgtgt
     6001 tgcgatggcg tcgtaggccg gcgcggcaaa gttcgccagt tgctggccga aaagcagccg
     6061 gcttcgcacc atgaccgtac catggcggac gagctgttcg tcccaggcgt cgagcaaatc
     6121 cccggtgacc tggtcttgtt tcagcagctc gttgcgctgc ctcagcgcct ggcgatactg
     6181 ctggagggcg cgcaaatagg gcaagtcgag ctgggagagg gccatgtcaa gaaagcggcg
     6241 gcgcaccgat gcggttcccc gcaccagctc ggcgtcttcc ggtgaaaaaa acacgaccgg
     6301 cagcctgccc aggatgtcgc tgacccggga ttggctgacg ccgttgactt tgaaacgttt
     6361 ttcgccgcgc caccaggttg cctcaacaac cacgttccga tccgagcgct gtgcagccgc
     6421 gcggatgcga aagccgtggc atccatgctg tacgagatcg gtttcctggc tggtgcgatg
     6481 gctcttggag gtggcagcgt aaagcactgc ttcgagcagg gaagtctttc cctgcgcgtt
     6541 atcgccgaag atgacgttca gaccggggtc cggcgcgaaa gtcaggagcg gcaggcatcg
     6601 aaagccctcg gcgtgcagtt cgcgcaggac catggcgcac cgggaactca cgacatggct
     6661 tcgatgagtt cttccacttc cggtatcgaa gcgaagaaat cgtccataat cgcggggatt
     6721 ttcgcgcttt cgacccccag gagggcccag gattggctgg tatcaccggc cacggcgccc
     6781 tcgtacgacg gaaagcccgc ctcgatacag atggcgtcgg caacgttcag cagggccgcc
     6841 agacggcgcg aatcctcatt ctcgcagcgg gacaggtcat gatgggccag gatcgctgat
     6901 gcgattgact ccggcaggcg ccagttcatg gccagacggc cgcctacttc cgcatgatcg
     6961 gcaccgatga tttcacgttc cacctggtag gccggaacct ttcggtcccg gctcaacgtt
     7021 tccgcctcca taagatcatc agacaggtag tcttcgagga tgattttgcc gatgtcgtgc
     7081 agcaatccat agacgaacgc ttcatcgggc tgcaacgcca gcgcgacacg gcccgtacgc
     7141 agcacggccc gcgcggccaa tccggcaacg atgctgtgct gatacaacag gccggcactg
     7201 cggctgagcg tatcaaaaac cgtggccgta agcaccaggt tgtggatgac tttgattcca
     7261 agaagggcta cggcatgctc aaccgacgtc acgtgctccc gcaagccgta cgccgcggaa
     7321 ttggccagac gcagggtttt cagtgcgact gcggggtcct gggtgatggc tttgcccacg
     7381 tctgacagag acgattcggg atcattcagc agctgcacga tccgggccag ggcgcccgga
     7441 aggctgggca gcgtgatgat ctcttcaagc aggaattcaa tgtctcgaga cgtgttcacg
     7501 atgcatccag agctggcagg ccgcactcgc cgaactggcg ctcctgggcg cataaaatcg
     7561 ccacgacgtc ggcgggcgcc agcctttcca ggattgtggc aaggggaagt gactcgaccg
     7621 gtccctgttc ctcatcctgg aacacgacca aggctccgcc gggcgcattt cgcggcccga
     7681 ccagcacgac ttgcccccgc gcgccatcgc aaagagtgag cggaccgacc acgc
//
LOCUS       DAOPBQ010000105         7708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8606_length_7708_cov_3.568405, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000105 DAOPBQ010000000
VERSION     DAOPBQ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8606_length_7708_cov_3.568405"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKW60_04935"
     CDS             complement(<1..365)
                     /locus_tag="PKW60_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98807.1"
                     /translation="MLKLVLENPWVQAVGMLLALVGAGLLIYLLSPVLIPLLGAFMFA
                     YLLDPVVDYFENAEGKNRLFRWRLPRGVTIFALGVVALLLLLSIPVVVIPQLILESRA
                     LLAVATVEEAQAAVDAASAA"
     gene            458..1564
                     /locus_tag="PKW60_04940"
     CDS             458..1564
                     /locus_tag="PKW60_04940"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HPJ98808.1"
                     /translation="MRTNRFILAGVASLAAVLTAASGDVLSLTRGDLVNGTLRDLSDG
                     IVVFDTGLAGQIIVPVTQVRSLSTDSPFFVHLGDGRRLEGRFAGSGVITRIVPENGGE
                     AAVLSLAAVKNIEPAPDKHAEAASPDTLAPAQFSLETGYLHRWANEDYDAAFARLTLR
                     KQAGTFGLFSENLFELSDDERFPRLFRSSVDWRFPADSSLLPELGIEAERDLDAGLAF
                     RGSLHAGLARAFIDEPGRLFELTGGLNAAFEYYDASELAQSSGSRLRQFVRRLYYADE
                     WQREQNQDINLRLRLRFHQIIRESLLTQTISLHPSLTDFGELRARSESSFRMPLTSQL
                     NFRFDLLFDYDNEPHYEWTDSFRTFIGAGFEWDF"
     gene            1945..2892
                     /locus_tag="PKW60_04945"
     CDS             1945..2892
                     /locus_tag="PKW60_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98809.1"
                     /translation="MSAVIETQDLTKVYKGMSKAHDLVALNKLSIQVNEGEIFGYLGP
                     NGSGKTTTIKLLLSLIFPTEGSMKIMGNANITSPEVRKNIGYLPEGAYYPDFLQGEEI
                     LNYYGNLYGMKGPGLKKRIDEVLEIVGMTRARKRLIRGYSKGMRQRIGLAQALLSNPS
                     ILILDEPTTGLDPVARKEIRDLLIELRDQGKSLLISSHELLEVELISDRVGILFEGVL
                     QTSGTIDELLTSREFSITAEDASAEAIQNVREAGLLAEDAAAGKLALRVPENMSLYEA
                     LDICKSQHMKIVSVSPRRETLEEVFVRVVGSAVSSKEAQ"
     gene            2905..3804
                     /locus_tag="PKW60_04950"
     CDS             2905..3804
                     /locus_tag="PKW60_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPJ98810.1"
                     /translation="MSIWLASNPWLTSLYGVIVLAILASVAYFTPWSIALFTFREGLR
                     KKTLIGFLILSILVIFGSMFMTAMMYDTGGTVATDVDVKLIKDICITAISIFGVLIAI
                     FISASVVPTEIENKVVYTVLSKPIRRFQYLLGKFVGVQIIVLVNLLLMGVLFSIALYV
                     KEPIFPRMLLYAIFLTYFEFLIVSAFTFAVAAASTSAVLPTIAGLFIYITGNLTQYLE
                     SVVNRSEVSENAADKALGGLAWVLRNTLPDLQYFSLKTQILGLQINDTPKDIQIPNLA
                     VYGILYALAGYIIAYWVFRRKEL"
     gene            3811..5607
                     /locus_tag="PKW60_04955"
     CDS             3811..5607
                     /locus_tag="PKW60_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98811.1"
                     /translation="MKRFPNLVLAILVAIGLALGALQGYRVRNLREADAFYRWLVTAS
                     TQLRVFGSITPELEGEEAEKYMDRDLFDRAVAKADEVLPDIPVMDGDIDDATGTPYPK
                     LVRYTRTLSTGSHTVEGESGSAAVAANPEQVKRDAEVWRFARSASLAPERADFTRFLR
                     DKRLATAGAQIGVADIYRNQETPVSIGQMFLGFRKLAAGLIWIEVDEAFHKGEMQRMV
                     PLMRTTVLLDPNFVDAYLIGAWHLAYNLTAQMPDTPGYLKEYVPEFGAWVGNKERAYF
                     AAANFLKDGIRNNPRDYRLYFDLGYAVYAEKLNDHMNAVTYLTEATKQRHESWVRRML
                     YHSLQATGQFEEARAGWEDYLRANPGHPVAQRMIPTNTALMKERDAEALDAQAQKLLT
                     QAAQARADGQEAQTAQLERQAGELEKQREALLDEARAIYTDLNANTAEGDEFAQARLW
                     RMQAIELHKQGRTLEALALLRQARMEVSRFFHEASDLIIRMCIEADLPLNLSERKRLL
                     EVERMKEAWAEKPVEVAGRRFDFSLAEDTWHQAGYADQETITLTPATRDLAALRKNRP
                     VIDQFIGLGDNVVFELDGQWYEFHKQNLFGNI"
     gene            5710..6387
                     /locus_tag="PKW60_04960"
     CDS             5710..6387
                     /locus_tag="PKW60_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPJ98812.1"
                     /translation="MPVPQAIIERNLAGLRQRIAAASQRAGRSAGAIRLVAVTKSVGA
                     EEVQALLELGVTDFGESRPLQAREKLERFRQMASWHMIGTVQRRKCPEIVRWFDCVDA
                     VDRIEVAEALHQRCEESGRGVPLPILIEVNVSGEASKHGFAPDQLPAVLDRLRSLSSL
                     QTAGLMTMAPFSDRPEDSRPFFARLRELADAHRLAEVSMGMSGDFEVAIEEGATQVRI
                     GSALFKS"
     gene            6409..7425
                     /gene="pheS"
                     /locus_tag="PKW60_04965"
     CDS             6409..7425
                     /gene="pheS"
                     /locus_tag="PKW60_04965"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPJ98813.1"
                     /translation="MKHKLEEIRASALETIHAAADLAALEQLRVKYLGRKGELTEILR
                     GLAQCPPEDRPLAGKAANEVKLAVADALEARKEAISKSLEAASATAERLDLSLPGRRP
                     LRGHKHPVMRVAEEICDIFIQLGFQIASGPDVETEYYNFDSLNTPADHPARDLHDTFF
                     VKPGVVLRTHTSPVQMRVMEQTQPPVAVIVPGRVYRVDQDASHSPMFYQIEGLLVDKG
                     ISFADLKGVLQLFIQRYFGPTTRTRFRPHYFPFTEPSAEVDISCTVCSGQGCRVCKQS
                     GWLEILGCGMVDPEVFKYARYDSETYTGFAFGMGVERIAMLTHAINSIHYLYENDLRF
                     LEQF"
     gene            7427..>7708
                     /locus_tag="PKW60_04970"
     CDS             7427..>7708
                     /locus_tag="PKW60_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPJ98814.1"
                     /translation="MRISLNWLREWVDIGSDVDTLAHELTMLGLEIEAIEHLGAGLSN
                     VLVGQIKTIEPHPNADKLVVCTTDIGQNQPLQIVCGATNMRVGDKVPTAV"
BASE COUNT         1591 a         2451 c         2115 g         1551 t
ORIGIN      
        1 gcggcggacg ctgcgtccac tgcggcctgc gcctcttcga cagtcgcgac cgcaagaagc
       61 gctctggact caagaatgag ctgcggaatt accaccacgg ggatgctcaa cagcaacaga
      121 agcgccacta cccccagcgc gaatatggtc actccccgcg gcagccgcca tcggaaaagc
      181 cggttcttcc cctcagcgtt ctcgaagtag tccaccaccg gatccagaag atacgcgaac
      241 atgaaggcgc ccaataacgg aatcaagacc gggctgagca ggtatatcaa aagccccgcc
      301 ccaaccagtg ccagaagcat tccaaccgcc tggacccacg gattttccag aacgagcttc
      361 agcatgcccg aaaacctccg ctgcagcctt cacaaaaacc gcccgccgac ttgccaaaaa
      421 cggcgcggca ttctaccatt tatagtgagg acacatcatg cgtacgaatc gattcatcct
      481 ggcaggggtc gcctcgctgg ctgcggtctt aacggccgca agcggggatg tcttgtctct
      541 gacccggggc gacctggtca acggcacgct tcgcgacctg tccgacggga tcgtcgtctt
      601 cgacaccggc ctggctggcc agatcattgt gcccgtcaca caagtgcgct cgctgtcaac
      661 cgacagcccg ttttttgtgc atctcgggga tgggcgccgg ctggagggcc gctttgcggg
      721 aagcggcgtc atcacgcgca tcgtgcctga aaacggggga gaggccgccg tactttccct
      781 ggccgcggta aaaaacatcg aacccgctcc ggataaacac gcggaagccg catcacccga
      841 taccttggct ccggcccaat tctccctgga aaccggatat ctccaccgct gggcaaacga
      901 ggactatgat gcggccttcg cccggcttac cctgcgcaaa caggccggca cattcgggct
      961 gttttcggaa aatcttttcg aactttcaga cgatgaacgt tttccccgtc tgttccgctc
     1021 gtctgtggat tggcgtttcc cggccgactc gagcctgttg cccgagctgg gcattgaggc
     1081 cgagcgcgac ctcgatgcag ggctcgcgtt tcgcggcagc ctccatgccg gattggcccg
     1141 ggcctttatc gatgagcccg gacgcctctt cgagctcacc ggaggcctca atgccgcctt
     1201 tgaatactat gatgcgagcg agctcgcgca aagctcggga agccggctcc gccaattcgt
     1261 gcgccgcctg tattacgcgg atgaatggca gcgtgaacaa aaccaggaca tcaatctgcg
     1321 cctgagactc cgcttccacc agatcatccg ggaaagcttg ctcactcaga cgatttctct
     1381 ccatcccagc ctgactgatt tcggcgaact ccgggcacgc tccgagtctt cgttccggat
     1441 gcccctcaca tcccaactga atttccgatt tgacttgctt ttcgattatg acaatgagcc
     1501 ccattatgaa tggacagaca gtttccgcac tttcatcggt gcaggttttg aatgggattt
     1561 ctagattgcc cggcatgcgc gttgcgccct gtgacgctgc tccctgaccg cctcggccag
     1621 cgacaccgcc tcgactccgc cgccctgact atcgccaaca ttcgtgtgcc ggcagattcc
     1681 cagttccttc tccaaacgcc cggattcgag cagaatcgcg tccgcgtcaa tggcctcggc
     1741 cggatgcatt tcttgcgcga ggcaacgatg ccgtttctgc tgctttcatt gaaagcaatc
     1801 cgctcgattg acatgcccgg tgccctctgt taccattcgt gcctgatcct gtgtttcatc
     1861 ttgcttttct gacacagtgg cgccccgtaa cgttggcttg ttccggcagt catttctgcc
     1921 gggcgcagaa tggagtgagt ttccgtgtct gcagtcattg aaacccaaga cctgaccaaa
     1981 gtgtacaagg gcatgtcaaa ggcccacgat ctcgtcgccc tgaataaact ttccattcag
     2041 gtgaatgaag gcgagatttt cggctatctc ggacccaatg gttccgggaa aaccaccacg
     2101 atcaagctgc tcttgagcct catcttcccc accgagggga gcatgaaaat catggggaat
     2161 gccaacatca cctccccgga agtgcgcaaa aacatcggct acctgcccga aggtgcgtac
     2221 tatcccgact ttctgcaggg tgaggagatc ctcaactact acggcaatct ctatggcatg
     2281 aaaggccccg gcctcaagaa acgcatcgat gaggtgcttg aaatcgtcgg catgacccgg
     2341 gcgcgaaaac gtctcatccg aggctattca aaaggcatgc gccagcgcat cggcctcgcc
     2401 caggctctgt tgagcaatcc cagcatcctc atcctcgacg agcccaccac cgggctcgac
     2461 ccggtcgcgc gaaaggagat ccgcgacctc ctcatcgaac tccgcgacca gggcaaaagc
     2521 ctgctcatct ccagccacga actcctcgag gtcgaactca tcagcgaccg tgtcggcatc
     2581 ctctttgaag gcgtcctgca gacttccggc accatcgatg aactgctcac cagccgcgag
     2641 ttctccatca cggcggaaga tgcctccgct gaagccatcc agaatgtccg cgaggcgggc
     2701 ctccttgccg aagatgccgc ggccgggaaa ctcgccctcc gcgtgcccga aaacatgtcc
     2761 ctgtatgaag ccctggatat ctgcaagtcg caacatatga aaattgtcag cgtgtccccg
     2821 cgacgagaga ccctcgaaga agtcttcgtc cgggtcgtgg gttccgccgt gagcagcaaa
     2881 gaagcacagt gaggagagcg ttagatgagt atttggttag ctagcaaccc gtggctgacc
     2941 agcctgtacg gggtcattgt cttagccatt ctggcctccg tggcctattt cactccctgg
     3001 agcatcgccc tcttcacttt tcgtgaaggg ttgcgcaaga agaccctcat cggattcctg
     3061 atcttgagta tcctggtcat cttcggttcc atgttcatga ccgccatgat gtacgacacc
     3121 ggcggcaccg tcgctacgga tgtcgatgtc aagctcatta aagacatctg catcacggcg
     3181 atctccattt tcggcgtatt gatcgcgatc ttcatttccg cctccgtcgt tcccacggaa
     3241 atcgagaaca aggtcgtcta caccgtgctg tccaaaccga tccgccgctt tcagtacctc
     3301 cttggaaaat tcgtgggcgt gcagattatt gtcctggtca atttgctgct gatgggcgtg
     3361 ctcttctcca ttgccttata cgtgaaagag cccattttcc cgcggatgct tctgtatgcc
     3421 atttttctga cttatttcga atttctgatc gtgtcggcat tcacctttgc cgtggcggcc
     3481 gcctccacct ccgcggttct gccgaccatt gccgggctct tcatctacat caccggcaac
     3541 ttgacccagt accttgaaag cgtcgtcaac cgctccgagg tctccgagaa cgccgccgac
     3601 aaggccctcg gcgggctggc ctgggtgctc cgcaacacgc tgcccgatct gcagtatttc
     3661 agcctcaaaa cccagatcct cggcctccag atcaacgata ccccgaaaga catccagatc
     3721 cccaacctcg ctgtttatgg cattttatac gccctggccg gttacatcat tgcctattgg
     3781 gtcttccgtc gtaaggagct gtaatccatt atgaaacggt tcccgaatct cgttctggcc
     3841 atattggtcg ctatcgggct cgccctgggc gcattgcagg gataccgcgt gcgcaacctg
     3901 cgcgaagccg atgcctttta ccgctggctt gttaccgcgt caacccagct tcgtgtcttt
     3961 ggctccatca cgcctgagct cgaaggcgaa gaagccgaaa aatatatgga ccgcgacctc
     4021 ttcgaccggg ccgtggccaa ggccgacgag gtcctgcccg acatccctgt catggacggc
     4081 gatatcgatg acgccaccgg cacgccctat cccaaactcg tgcggtatac ccgcaccttg
     4141 agcacgggca gccacacggt ggaaggggag agcggcagcg cggccgtggc agccaatccc
     4201 gaacaggtca aacgggacgc cgaagtctgg cgcttcgccc gcagcgccag cctcgcgccg
     4261 gaacgggctg atttcacccg cttcctccgc gacaaacgcc tcgcgactgc cggcgcccag
     4321 attggtgtcg ccgacatcta caggaatcag gaaacccctg tcagcatcgg ccagatgttc
     4381 ctgggcttcc gaaaactcgc cgccggtctc atctggattg aggtggacga ggcgtttcat
     4441 aaaggcgaaa tgcagcggat ggtgcccctc atgcgcacca cagtcctgct cgaccccaac
     4501 ttcgttgacg cctatctcat tggcgcctgg catctcgcgt ataatctcac ggcacaaatg
     4561 ccggacacgc ccggttatct gaaagagtac gtccccgaat tcggcgcgtg ggtcggtaac
     4621 aaggagcgag cctacttcgc cgccgccaat ttcctcaaag acggcattcg caacaaccct
     4681 cgcgattacc ggctctattt cgacctgggc tatgccgtat acgccgaaaa gctcaacgac
     4741 cacatgaatg cggtcacgta tctcaccgaa gcgaccaaac agcggcatga gagctgggtg
     4801 cgccgcatgc tgtatcacag cctgcaggcc accgggcaat tcgaggaagc ccgcgccgga
     4861 tgggaggact acctgcgcgc gaacccgggc caccccgtgg ctcaacgcat gatccccacc
     4921 aataccgcgc ttatgaagga acgggacgcc gaggcccttg atgcccaggc gcagaagctg
     4981 cttactcagg ccgctcaggc acgggcggat gggcaggagg cccagaccgc ccagctggaa
     5041 cgtcaggccg gggagctcga aaagcagcgc gaggcgcttc tggacgaagc gcgggccatc
     5101 tacaccgacc tcaatgcgaa caccgctgaa ggcgatgaat tcgcgcaagc ccgtctgtgg
     5161 cggatgcagg cgattgaact gcacaaacag ggacgcaccc tcgaggccct ggccctgttg
     5221 cggcaggcgc gcatggaagt ctcgagattc ttccatgaag cctctgacct tatcattcgg
     5281 atgtgcatcg aggcagacct gcccctcaac ctctctgagc ggaaacgcct cctcgaggtc
     5341 gaacgcatga aagaggcgtg ggccgaaaaa cccgtcgagg tcgcgggccg ccgcttcgac
     5401 ttcagcctcg cagaggacac ctggcatcag gccgggtacg ccgatcagga gacgattacg
     5461 ctcaccccgg cgaccaggga tctcgcggca ctgcgtaaaa accgccctgt catcgaccag
     5521 tttatcggac tgggcgataa cgtcgttttc gagctggacg gccagtggta cgaattccac
     5581 aaacaaaacc tgttcggaaa catctaaata gctccacctt gcgggcaagc ccaggttgat
     5641 ccgctgttcg cgcggcacta tcataagggc ttattcattt cgggttgaat cgctggggag
     5701 tcgtcgcgcg tgccagtacc gcaagccatc atagagcgca atctggccgg gctgcgccag
     5761 cgcatcgcgg ctgcttccca aagggccggg cgcagcgccg gcgccattcg gctcgtggcc
     5821 gtcacgaaat cggttggagc ggaggaagtc caggccctgc tcgaactcgg cgtgaccgac
     5881 ttcggagaaa gccggccgct gcaagctcgc gagaagctcg aacggtttcg tcagatggcc
     5941 tcctggcata tgattggcac cgtgcagcgg cgcaagtgcc cggagatcgt gcgttggttt
     6001 gactgcgtgg atgccgtcga ccgaattgag gtcgctgagg cgttgcatca gcgttgcgaa
     6061 gagagtggga ggggagtccc cctgcctatc ctcatcgaag tcaacgtatc cggcgaggcc
     6121 agcaaacacg gtttcgcccc agaccagctc ccggcggtgc tggaccggct ccggagcctg
     6181 agcagcctgc aaacggctgg cttgatgacg atggcgccct tctcggaccg cccggaggat
     6241 tcacgcccgt tcttcgcccg gctgcgggag ctggccgatg cccatcgtct agccgaggtt
     6301 tcgatgggca tgtccggcga ttttgaagtt gcaatcgagg aaggcgctac ccaggtgcgc
     6361 ataggctcgg cgttattcaa aagttaacca catgaggatt gccggtgaat gaagcacaaa
     6421 cttgaggaga tccgggccag cgccctggaa accatccacg ccgcggccga tttggccgcc
     6481 ctcgaacagc tccgggttaa atatctcggg cgaaaaggcg aactcacgga aatcctgcgc
     6541 ggactggccc aatgcccccc cgaagaccgg ccccttgccg gaaaagccgc gaatgaggtc
     6601 aaattggcgg ttgcggatgc cctggaggcg cgaaaggaag ccatctccaa atctctggag
     6661 gctgcgtcgg ccacggcgga gcgcctcgac ctgtctcttc ccgggcgccg gccacttcga
     6721 ggccataaac acccggtgat gcgcgttgcc gaggaaattt gcgacatttt catccagctg
     6781 gggtttcaaa ttgcctcagg accggatgtt gagacggaat attacaattt cgacagcctc
     6841 aacaccccgg cggatcaccc cgcgcgcgac cttcacgaca cctttttcgt caagccgggg
     6901 gtggttttgc gcacccatac gtcgccggtg cagatgcgag tcatggagca aacccagccg
     6961 cccgtagccg tcatcgtgcc cggacgcgtc taccgggttg atcaggacgc cagccacagc
     7021 cccatgttct accagatcga agggcttctg gtcgataaag gcatcagctt cgccgacctc
     7081 aaaggggttc tgcaactctt tattcagcgc tactttggac ccactacccg gacgcgcttc
     7141 cggccgcatt attttccttt taccgaacct tccgccgagg tggacatctc ctgcacggtc
     7201 tgttcgggcc agggatgccg tgtctgcaaa cagtccggct ggcttgaaat cctcggctgc
     7261 ggtatggttg atcccgaggt tttcaaatac gccaggtacg attctgaaac ctacaccggt
     7321 tttgccttcg gaatgggcgt tgaacgcatc gccatgctga cccacgcgat taacagcatc
     7381 cattacctgt acgagaacga tttacggttc ctggagcagt tttaacatgc gcatttcact
     7441 caattggtta cgcgaatggg tcgatatcgg ctccgacgtg gacacgcttg cccatgagct
     7501 cactatgctt gggctggaaa tcgaggccat cgaacacctt ggcgccggcc tgagcaatgt
     7561 cctcgtcggc cagatcaaga ctatcgagcc gcaccccaat gccgacaaac tcgtcgtctg
     7621 caccaccgac atcggccaga atcagccgct ccaaatcgtt tgcggggcga ccaacatgcg
     7681 cgtcggcgac aaagtcccca cggccgtc
//
LOCUS       DAOPBQ010000106         7664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8665_length_7664_cov_4.003548, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000106 DAOPBQ010000000
VERSION     DAOPBQ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8665_length_7664_cov_4.003548"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKW60_04975"
     CDS             complement(<1..345)
                     /locus_tag="PKW60_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98815.1"
                     /translation="MFRRRKAEKLMAQALYESLPEHDAAFLEAYFQAHPAAREEMEAL
                     RRFVADMPTEKPQLNADLIPAVRARIEEQSGRLEKKPRLRPAAAIAALVFVAAGFFLL
                     KLPDAAPPGHLAD"
     gene            complement(399..995)
                     /locus_tag="PKW60_04980"
     CDS             complement(399..995)
                     /locus_tag="PKW60_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPJ98816.1"
                     /translation="MNQDADRNWAAQDDDALAMAVKAGELAAYDELIRRHQARVYAVA
                     YRFAGNREDALDIAQEALFKAYRKLGGWEPRTGFVPWLMRVTTNHAIDHLRRSKRRRT
                     ARLTDEYPGREADTLTGHSAAQPEAAARAGEIGRHVHQALERLSNAQRNVFVLRHYEG
                     LQLAEIAEVLECSIGSVKVHLFRAVRKLRLELKDIGHF"
     gene            complement(1040..2080)
                     /locus_tag="PKW60_04985"
     CDS             complement(1040..2080)
                     /locus_tag="PKW60_04985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPJ98817.1"
                     /translation="MNNPSEAAPREKKLSTPAVSVVMPCFNAAGTLETAIESIQNQTF
                     QDWELIIVDDGSLDDSLSVMKSAAASDERLRLVGQAHSGIVRALQAGCALARGSFIAR
                     MDADDIAQPERLARQYTYMQEHPDVALCGTHVSMFGGRIGVGRARYEAWINGLRSHGD
                     IARELFIECPVAHPTFFLRRAPFERAGGYEEHGWAEDYDSCMRLFLAGARFGVVPDPL
                     LRWRESPGRLSMTAERYSPARFRALKRFYLARSYLRGRKRFHQWGAGEVGKVWLREWE
                     DLRPEAVVDINPRKIGRKIHGVRVIAPDELPEPGLTFTLIAVGAPGARDEIRAWLKPR
                     GYIELQDFLFVA"
     gene            complement(2144..2845)
                     /locus_tag="PKW60_04990"
     CDS             complement(2144..2845)
                     /locus_tag="PKW60_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98818.1"
                     /translation="MQAEDRPIHFSAELIHWPTQHKIPVLQKLYFDLSQIREAAYDST
                     DFSVPGRFRFFSKRGNKTLSTFLFLPDRFMLAEEWVDLPLSDFLTKLERAAGLAMDAL
                     SIPFFPAHTVTIRTTFALTHHNEARRFLLDHVCNQKDRLKPFFDRPIAVGGIRLVFPE
                     TPLHLHTYNVAIESFRHSEFEVYVEVKGVFTRERVMRDNLGQIADNFRMTRDFISDRI
                     FPFLNQYDTPEEDQA"
     gene            complement(2956..5358)
                     /locus_tag="PKW60_04995"
     CDS             complement(2956..5358)
                     /locus_tag="PKW60_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98819.1"
                     /translation="MRMHCRLTWTGMLLAAMLTGLPTLQGHAAETPGRLDLSGAVIVV
                     RPGERPSAEHMASTVLIEEVGKRTGLQWTETASWPEDTAPVIAITVAGATPELTRMAP
                     VRSGEGLPEQKPEGFRLYVEKRDGAAPTVWLIGADARGALYAAAHFLRTMTWSEGSVS
                     FPAEYDLCTAPAYPIRGHQLGYRHTANAYDAWDVATYDQHIRELVLFGANCVENIPSQ
                     DEPGPHAQLSRAEMNVAMSKICQRYGIDYWAWIPAEFDLNDTARRDHSLEELNDIFQS
                     TPLLNAVFIPGGDPGDNPPDLLMPYAADMAARLIQHHPDAKIWISLQGFGPDRVDSFY
                     AWLDAHQPDWLGGVVHGPSSPPIAETRRRLPKQYGLRHYPDITHSLVCQYPVYWWDQA
                     FALTLGREGPNPQPAWYTHVHNWYAPWTDGFLSYSDGINDDVNKAVWSRVSWDPSIEP
                     RQTCIEYANFFFGSAVAERAADGILALEKNWEGVLVDNGAVDATLMQWQALEAVLPRL
                     GNNWRWQLCLLRAYYDAYTRHRLLYETQLEADVNALLAKAPALGAAAAMEQAEAILKL
                     AETGPVKKEWRQRIEELCVALFDSIGYQTSVPRFQAKNGERGAILDYVDRPLNNRWWL
                     EDEFKKIREFATEEEKLARLEVIRTWESPGLGSYYDDIGNVAQMEHVALPAGPWVEPD
                     LVEMRNPHFPWTENGFSRARLSWLCLMRWPEKLTYRGLNPEAEYFLRATGRGGLRPIV
                     DGVALVPSAYPREQGAFKEFPIPPELYADGEIAVTFGDIDEYHLNWRLWSYLAEIWLI
                     KK"
     gene            complement(5651..7447)
                     /locus_tag="PKW60_05000"
     CDS             complement(5651..7447)
                     /locus_tag="PKW60_05000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPJ98820.1"
                     /translation="MKRVRSLLILSIVILLGCAHADAAPTVRESVASIIDRLRTAVSP
                     EELVSLTPEQALPYLTPEEKTILGSDYITFTVNVPVVVSVFRDVTQGEVPFWLLEREF
                     QLGSEQVQVDKRTFEIWRKNFPAGKIGLGVNSISGAGRHYFVGIAPAHPGAHLNVTNI
                     HPEQHTLGEMKVGERVYAGSGDEIAALPASLEGQILLRGDSLRRRDAQITGIFQVTEF
                     PATIQPDQILLTWSADPKTTQTIQWRTNTEVASGSVRYIESTRAAGAEAADWKEVAAV
                     TAPLENSILVNDPICHRHTAVLTGLQPGTTYRYAVSDASTQGWSAAAEFTTAPPKPIS
                     FSFIYQGDVQAGFDGWGKLMDTAREKHSYAAFHLLAGDLVNRGNERNFWDSFFHYSGG
                     IFASRPLVPCLGNHEYQYDQGPWMYLQILTLLENGPKTVPPECAYTFEYSNVLFVVLD
                     GNLPASDQAPWLEEQLAKSNATWKIALFHQPVYSAGPRRDNPEIRMLWGSLFDKYHLD
                     LALQGHDHSYLRTYPMFGGERVATPAEGTIYIVSFSGMKMYELGEHDYTEFGMEHVPT
                     YQVLDIDVETNTLTYKAYDVEGTVRDEFVIQK"
BASE COUNT         1527 a         2329 c         2233 g         1575 t
ORIGIN      
        1 gtctgcaaga tggccgggcg gcgcggcatc cggcagttta aggaggaaaa aaccagcggc
       61 aacaaacacc agcgcggcga tggcagcggc ggggcgaaga cgcggtttct tttcgagcct
      121 gccgctctgc tcctcaatac gcgcccgaac cgccgggatc aagtcggcat tcagttgagg
      181 cttctccgtg ggcatatccg ctacaaaccg gcgcaaggct tccatctcct cgcgcgcggc
      241 gggatgcgcc tgaaaatagg cctccaggaa agcggcatcg tgctcaggca aggattcgta
      301 gagtgcctgt gccatcagtt tttccgcttt gcgtcgtcta aacatgaatt caatatcctc
      361 aaaccgcgtt cagggcaata cagcccacgg cagctttctc agaagtgccc gatatccttg
      421 agttcgagtc tcaactttcg cacagcgcgg aacaaatgga cttttacgct gccaatgctg
      481 cattcaagta cctccgcgat ttccgcgagc tggagccctt catagtgcct caacacgaac
      541 acgttgcgtt gtgcgttcga gagcctctcg agcgcctgat gcacatggcg tccaatctcc
      601 cctgcgcggg ccgcggcctc aggttgcgcg gcgctgtgcc ccgtgagtgt atccgcctcc
      661 ctgccgggat actcgtccgt caggcgcgcc gttcttcgcc gcttactgcg ccgcaggtgg
      721 tcgatggcgt gattggtcgt cacgcgcatc aaccacggca caaaccccgt ccggggctcc
      781 cagccgccaa gcttccggta cgccttgaac aaggcctcct gggcgatgtc cagggcgtct
      841 tcccggttac cggcaaaccg gtaggccacc gcatagacgc gcgcctgatg tcgccggatc
      901 agttcgtcat aagccgctag ctcaccggct tttacggcca tcgccagcgc gtcgtcgtcc
      961 tgggcggccc agttcctgtc agcgtcctga ttcacggcta taacgatttc ccggccaata
     1021 gagttaacac accctgacct tacgccacaa agaggaaatc ctgcaactcg atatagccgc
     1081 ggggtttcag ccaggcgcga atttcgtcgc gcgcgcccgg tgcgccgacg gcaatgaggg
     1141 taaatgtcag gccgggctcc gggagctcat ccggagcaat tacccgcacg ccatgaattt
     1201 tccggccgat cttgcgcgga ttaatgtcca caaccgcttc gggtcggaga tcctcccact
     1261 cacgcagcca gaccttgcct acctcaccgg cgccccattg atgaaaacgt ttgcgtccac
     1321 gaaggtacga acgtgcgaga taaaagcgct tcaatgcccg gaatcgagcc ggtgaataac
     1381 gttccgcggt cattgacagg cgcccgggcg attccctcca gcgcagcagg gggtcaggca
     1441 ccacgccgaa ccgcgctccg gccaaaaaca gccgcataca tgaatcatag tcttccgccc
     1501 agccgtgttc ttcgtagccg ccggcgcgtt caaagggtgc gcggcggagg aagaacgtgg
     1561 gatgcgcgac tggacattcg atgaacagct cgcgtgcgat gtcgccatgg ctccgaagcc
     1621 cattaatcca cgcttcatac cgcgcccggc ccacgccgat gcgcccgccg aacatgctga
     1681 cgtgggtgcc gcacagggcg acgtccggat gctcctgcat gtacgtgtat tgacgggcaa
     1741 gccgttcggg ctgggcgatg tcatccgcgt ccatgcgcgc gataaacgac ccccgggcaa
     1801 gagcacaccc cgcctgcagc gcccgcacaa tgccgctgtg cgcctgcccc accagccgga
     1861 ggcgctcatc gctggccgct gcggacttca ttacgctcag agaatcgtcc agggagccat
     1921 catccacgat gatgagttcc caatcctgaa acgtctggtt ttggatggat tcgatggcgg
     1981 tttcgagggt tcccgcggcg ttaaagcaag gcatcaccac ggaaaccgcc ggcgtggaca
     2041 gttttttttc gcgtggcgct gcttcgctcg gattgttcat gtctgcaaac atacggcggg
     2101 ctgtatgtgg ctgcctcagc ttgacttggc cacgccagcg cggctatgcc tggtcttctt
     2161 cgggcgtgtc gtactgattc agaaatggaa agatgcggtc cgaaataaag tcccgggtca
     2221 tgcgaaaatt gtccgcaatc tggcccaggt tgtcccgcat cacgcgctcc cgggtaaaaa
     2281 cccccttcac ctctacatat acctcgaatt cgctgtgccg gaaagactcg atggctacgt
     2341 tatacgtatg aagatgtaac ggggtttcag gaaacaccag ccggatgcct ccgacggcga
     2401 tcggacggtc aaaaaagggt ttgaggcgat ccttttggtt acagacgtga tccagcaaaa
     2461 agcggcgcgc ctcgttgtga tgagtcagcg cgaacgtggt gcgtatggtc acagtgtgtg
     2521 cgggaaaaaa cggaatggac aaggcgtcca tggccaggcc ggcagcccgc tccagcttcg
     2581 tcagaaaatc cgatagcggg aggtcaaccc attcctcggc cagcatgaag cgatccggca
     2641 gaaacaaaaa agtcgagagc gtcttattgc cacgcttgga aaaaaaacga aaccggcccg
     2701 gcacactgaa atctgtgctg tcgtaggctg cttcccgtat ctgcgacaga tcgaaataga
     2761 gcttctgcag cacaggaatt ttgtgctggg ttggccaatg gatcaattcc gcggaaaaat
     2821 ggatcggtct gtcttcagct tgcatcaata ctcccctgtc tcaggtcagt ataggcctct
     2881 ggacctggca gcgcaacctg gcgtgaacaa aggactttca cgccagttgc cgctgcaggg
     2941 agcctggtgc tgctgctatt tttttataag ccagatttcc gcgagatacg accacagccg
     3001 ccagttgaga tggtattcat cgatgtcccc gaacgtgacg gcaatctcgc cgtcggcata
     3061 cagttcgggc ggaatcggga attctttgaa tgcgccctgt tcccgggggt aggccgaagg
     3121 caccagggcg actccgtcca cgatcggccg caatccgccc ctgcccgtag cgcgcagaaa
     3181 atattccgcc tcaggattca gcccgcgata cgtgagtttt tcgggccatc gcatcaggca
     3241 aagccaggac aaacgcgccc ggctgaatcc gttttcggtc caggggaagt gcgggttgcg
     3301 catttccacg aggtctggct ctacccaggg acccgccggc agcgccacgt gctccatctg
     3361 tgccacgttt ccaatgtcgt cgtaatagct tccgagccca ggcgattccc aggtgcggat
     3421 gacttccagc cgcgcgagct tctcttcttc cgtagcgaac tcgcggatct tcttgaattc
     3481 gtcctccagc caccagcggt tattgagcgg gcgatcaacg taatccagaa tggcgccccg
     3541 ctcgccgttt ttggcctgga accggggcac actggtctga tacccgatcg aatcaaacag
     3601 ggcgacacac aattcttcga tccgctgccg ccactctttt ttaacgggcc cggtttccgc
     3661 cagtttcaag atggcttccg cctgttccat ggcggccgcc gcgccaagtg ccggggcctt
     3721 cgcgaggagg gcattgacgt ctgcctcgag ctgggtttca tagagaagcc ggtgccgggt
     3781 atacgcatcg tagtacgcac gcagcaggca caactgccag cgccagttgt tgccgagtct
     3841 gggcagtacc gcctccaggg cctgccattg catcagggtg gcgtcaactg ccccgttatc
     3901 gacaagcacg ccctcccagt tcttttcaag cgccaggatg ccatcggcgg cacgttcggc
     3961 gacggccgat ccgaaaaaga aattggcgta ttcgatgcag gtctgccggg gttcgatgga
     4021 tgggtcccag ctcacgcggc tccagacggc cttgttcacg tcgtcgttaa tgccatcgga
     4081 gtagctgaga aagccgtccg tccagggcgc gtaccagttg tggacgtgtg tgtaccacgc
     4141 gggctgcgga ttgggacctt cccggcccag tgtcagcgca aacgcctgat cccaccaata
     4201 gaccgggtac tggcagacga gactgtgagt gatgtccgga tagtggcgca ggccgtattg
     4261 ctttggcagg cgccggcgcg tctcggcgat cggcggactg gagggcccgt gcaccacgcc
     4321 gcccagccag tccggctggt gtgcgtcgag ccaggcataa aaactgtcca ccctgtccgg
     4381 gccaaaaccc tgcagtgaaa tccagatctt cgcgtcggga tgatgttgga ttaaacgtgc
     4441 cgccatgtcc gccgcatacg gcatcaacag gtcaggcggg ttgtcgcccg gatcgccgcc
     4501 ggggatgaag acggcgttca gcaacggggt gctttggaag atatcgttga gttcctcgag
     4561 ggaatggtca cgcctggccg tatcattcag atcaaattcc gccgggatcc acgcccagta
     4621 gtcaatccca tagcgttggc agattttcga catggccacg ttcatttccg cccgcgacag
     4681 ctgggcatgc gggccgggct cgtcctggct ggggatgttt tctacgcagt tggccccaaa
     4741 cagaaccagt tcacggatgt gctgatcgta ggtcgccaca tcccaggcat cataggcatt
     4801 tgcggtgtgc cggtagccga gctggtggcc gcggatggga taggctggag ccgtgcacag
     4861 gtcgtattca gccgggaagg atacggaccc ttcgctccac gtcatggtgc gcaggaaatg
     4921 ggcggcggcg tacagcgccc cacgggcatc ggcgccgata agccagaccg ttggcgccgc
     4981 gccatcacgc ttctcgacat acagccggaa tccttccggt ttctgctccg gcaggccttc
     5041 gccggaccgg actggcgcca tccgggtgag ttcaggcgtg gccccggcaa cggtaatcgc
     5101 gatcacgggc gctgtgtctt ccggccatga cgcagtctcc gtccattgga gcccggtccg
     5161 tttcccaacc tcttcgatca gaaccgtcga agccatgtgc tccgctgacg ggcgttcacc
     5221 gggcctgacc acaatcaccg cgccagataa atcgagccgt cctggtgttt cggcggcatg
     5281 cccctgtagg gtgggcagtc ctgtgagcat ggcggcaagc aacatgccgg tccaggtaag
     5341 ccgacaatgc atacgcatga ttgttcccct ttcgtgtgct tcgtgtgatg caaacagcct
     5401 accaaaggcg ggcaaaaaaa acaccttcag ccggatgtca cgcgttgcac gaggcacgtg
     5461 cggcctttgc gtcacgcaag gagcgcttga catgcactcc aggcagactc gtctccgtga
     5521 gggcagagca cgcagcgcac gtcacctcgt gggtgcccgg cagaggtcgt ttgttgcccg
     5581 gggttcccgc atttcagcgc cggccacggc atggcgtcag aaagcggtct cgccgccggt
     5641 tgtatccggc ttatttctgg atgacaaact cgtcccggac cgttccttcg acgtcgtagg
     5701 ccttgtatgt aagcgtgttc gtttccacgt cgatgtcgag cacctgatac gtcgggacat
     5761 gttccattcc aaattccgtg taatcatgct cgcccagctc gtacatcttc atgcccgaga
     5821 aagacacgat gtagattgtc ccttcagcgg gcgtagcgac ccgctcgcct ccaaacatgg
     5881 gatatgtccg caagtaggag tgatcgtggc cctgcagggc gagatcgaga tgatacttgt
     5941 cgaacaagga cccccacagc atgcgaatct ccgggttatc gcggcgcggt cccgcggaat
     6001 acaccggctg gtggaacaag gcgattttcc acgtggcgtt gctcttcgcc agctgctctt
     6061 ccagccacgg cgcctggtcc gaggccggca gattgccgtc aagcacgaca aacagcacgt
     6121 tgctgtactc aaacgtgtac gcacattccg gaggaacggt cttcgggccg ttttccagca
     6181 gcgtcagtat ttgaagatac atccaggggc cttgatcata ttggtattca tgatttccaa
     6241 gacagggtac gagcggccgg ctcgcgaaga tcccgccaga ataatggaag aagctgtccc
     6301 agaagtttcg ctcgttgccg cgattgacca ggtcgcctgc gagcaggtgg aaagctgcgt
     6361 aggaatgctt ttcgcgcgcg gtgtccatca gttttcccca gccgtcgaag cctgcctgca
     6421 cgtccccctg gtaaatgaac gaaaaggaga tcggcttcgg gggcgcggtg gtgaattccg
     6481 cggcggcact ccagccctgg gtgcttgcat cgctcacggc ataacggtag gtggtgcccg
     6541 gttgcagtcc tgtgagcacg gccgtgtggc gatggcagat gggatcgttt accaggatgc
     6601 tgttttccaa tggggccgtt acggcagcga cttctttcca gtccgccgcc tcagccccgg
     6661 ccgcacgggt cgattcgatg taacggacac tgccagacgc tacctccgta ttcgtgcgcc
     6721 actggatggt ctgagtcgtc ttcgggtcgg cgctccaggt gagaagaatc tgatccggct
     6781 gaatcgtcgc gggaaattca gtaacctgga aaattccggt tatttgggca tcgcggcgcc
     6841 gtaaggagtc cccgcgcaac aggatctgac cttccaacga cgccgggaga gcggcaattt
     6901 cgtcgccgga accagcatat acgcgttccc ccactttcat ttcacccagc gtgtgctgtt
     6961 ccgggtgtat attcgttacg ttgaggtgtg cgccaggatg cgccggtgcg atgccgacaa
     7021 aataatgcct gcctgcaccg ctgatcgagt ttactccaag ccctatcttc cccgcaggga
     7081 aattcttgcg ccaaatctca aaagtccgtt tgtctacctg cacctgctcg ctgcccagct
     7141 ggaactcacg ctccagcagc cagaacggga cctcgccttg tgtgacatcg cgaaataccg
     7201 atacgacgac cggaacgttt acggtaaagg taatgtagtc tgaacccaga atggtcttct
     7261 cttccggggt cagataaggc agggcttgct ccggtgtcaa cgagacaagc tcttccggag
     7321 acacggccgt tcgcagacgg tcgattatgg acgcgacgct ttcacgaacg gtcggcgccg
     7381 catcagcgtg agcacacccc agcaaaatga cgatcgaaag gataagcaag ctccggacac
     7441 gtttcatgac tcgatttctc cctagatgta ctgtgctcag tgatgtctca gtctgccata
     7501 ttccgttgcc agtataaaag cgggcccgcc gcgttcacgc ggcgggccac aggaattccc
     7561 catccctgga gaaacccgac gaggttcttt taaccccggc tgaaaattcc ctgaatcgtc
     7621 gtcatcggga atttctcttg cgggtatctc acgaactcaa cgtg
//
LOCUS       DAOPBQ010000107         7647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8691_length_7647_cov_3.869995, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000107 DAOPBQ010000000
VERSION     DAOPBQ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8691_length_7647_cov_3.869995"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2191)
                     /locus_tag="PKW60_05005"
     CDS             complement(<1..2191)
                     /locus_tag="PKW60_05005"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPJ98821.1"
                     /translation="MGKSELRHLIAGIDHLPATMKVVGRALSVLFDASPDLEAAGALF
                     AIDPALASCALRMANVPGANRASSETILLRDAVQAVGYEGLRRAVLGVTLTSLPVQSC
                     QQHLERLQRHALACATCAAKTAAHTRVVSPGEAYVAGLLHDVGRLALIAAMPDEYCAF
                     LENNEPAELDRLERAAFDVDHALVGKWMAERWTLPRHLSQAIWFHHLPPGTLLGDRPI
                     VHLVAIVGFANAMSAWIVDGGDKPSPAHLHDLRAHAARLGLEHADIESLHEQTRDAMS
                     QRLVLLESGAAGCAAAFKDLQKAASNVTLPGRTESAESLSLKKEIKWLQALNRLNTSL
                     RPSQSLSEVLTLIVATVREGLDAAPGMCCAFDEGNRHLVGMTWDKPDAPPQQFRIPLS
                     PSTQQELDEVELLALEALDELGIGMSENGWRGAERTEPVLHDGILMLPMVADGTTRGQ
                     IMFETESQKIENHSWDAFKLMAFAGACGAVLARHHAYGELRELSEDLADAIARNAGAL
                     SAGDEDSLAEFAIGASHALQRPLNIVASQAQQLLRRMRSPEETSAVESILEQNRALTK
                     LVNDLMAFARPGLSVPVPTVVNYVLHRLVTTVSDRLEAKGITIAEEYEEGLPRVGVDK
                     HQLEQALLNLIVNGEQAMSQTGGQLLLKTSANSDRSRVHIMVQDSGPGVSAKHAESIF
                     EPFVSLREGLSGTGLGLAVCRAVVRKHGGEIRLLNPDMAGAAFEITLP"
     gene            complement(2302..2721)
                     /locus_tag="PKW60_05010"
     CDS             complement(2302..2721)
                     /locus_tag="PKW60_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98822.1"
                     /translation="MVDLLIVVVPLVAVALLSGAALRYLLRAWVDYRVRIAVLKSMDR
                     DADALTEPGGAGGDARPPSTPSSDGRVDFLITGIALGVIGACCALIGWNLRVGQLAVG
                     MYLGGIFSLCLGFLVGLAGLVARRLRSPRSRVNPPRT"
     gene            2899..3513
                     /locus_tag="PKW60_05015"
     CDS             2899..3513
                     /locus_tag="PKW60_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98823.1"
                     /translation="MKAFIKSVLLAGGIALALLLFTTRVLAPNFPRVVGRPAIDQVRS
                     FTWPGPERFSLENRDGTVHIWSHSAGEISVKARVRVYVTEQAQAAEAAAYAESLCAAS
                     SGAREIKMVTEPGERPDLLELRVDYEVFVPQGTDIEVLSVNGNVYVASGCGRVAIQGR
                     NADILVSKPSGEVTAKTTNGRINVADAPEGARLATVNGNIYAHV"
     gene            3601..3816
                     /locus_tag="PKW60_05020"
     CDS             3601..3816
                     /locus_tag="PKW60_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98824.1"
                     /translation="MNGGITVVLDEQFSGTVDARTTHGGIRSDIPLDTSSGIQRRHHL
                     RGNLGLGHAPLNLQTLNGNIWLARGRQ"
     gene            3813..4427
                     /locus_tag="PKW60_05025"
     CDS             3813..4427
                     /locus_tag="PKW60_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPJ98825.1"
                     /translation="MTTAITAAAGYWPGMDAMRATEQSDETLVKRAQAGDTEAFSELA
                     RRHELTVYNLSLRFMRNPSLAEDMAQEAFLKAYQKIGKFRRDSQFSTWLYRIACNVCL
                     SELQKRKRRGELSLAYTPELSGPGAAAPLPQAEEAELIRKCVARLPRRYAEAITLFYL
                     QECSYEEVAEIMEIPVGTLKTWLHRARGQLRRIVQHELRYEDEE"
     gene            4417..4869
                     /locus_tag="PKW60_05030"
     CDS             4417..4869
                     /locus_tag="PKW60_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98826.1"
                     /translation="MKSEDGPASDPLSRRIEAALRHETPAPLPGGFHSTLQRRLRVAA
                     MLERERQIFLHRLLHVAVPGVLGIAVAAGVILHGDVSQTFLYEFPGFLGHVDYLTALA
                     SRMWWLSDNPALLVSFGAIGMTLLIAGAFAGIFRSPAGSRQPAQGPIP"
     gene            5006..5470
                     /locus_tag="PKW60_05035"
     CDS             5006..5470
                     /locus_tag="PKW60_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98827.1"
                     /translation="MPVKYECPKCGRRFTEWGAEKVGFKCPKDQWCPKDHPDDVELVR
                     VGPSEEGASRRPTLKRTPRKLAQAKAPVMDLEEDEGMVPDMDELEGDDEFEDEDDETG
                     VIDDAEEEEATVEADAPDTVAVIDDDDAPDDAGADEDVDENVEDIIDDEELP"
     gene            complement(5636..6484)
                     /locus_tag="PKW60_05040"
     CDS             complement(5636..6484)
                     /locus_tag="PKW60_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98828.1"
                     /translation="MMRYAVLFVSMMMLVLPAVFAQESASPAGNQAETGAAPSSAVSP
                     ETPAAGVTPESGAASSAPAAVYEEPAQEPKGDTVILQDGRRFERVQVLRRTATDVEIE
                     IVEGVAPLSLPREVVKEILWDDYEPRTQFGAGKTPGASASAPRRTENALPEGTARDDQ
                     GNVEILPRVKVPAQLAGKLRTDITQLVRGLNGKDLFEALNLIKQATQLQIAADQPLRD
                     LASKSGGLKVNVTLNDDVKVNVAEFLENYLLKQKEMQSVGILYKPDRIIVTTREAAAK
                     LRQQEE"
     gene            6770..7147
                     /locus_tag="PKW60_05045"
     CDS             6770..7147
                     /locus_tag="PKW60_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPJ98829.1"
                     /translation="MALILIADDDSASFDVLAVALTAEGHKVLYAANGQEALEAAVEA
                     QPDLIFLDVMMPVFDGYETCERLRNDPGIPSKLPIIFLTATDEDPRKIQEVGGSDYLT
                     KRHMIAELQDMLVKHLGPKAFGD"
     gene            complement(7188..>7647)
                     /locus_tag="PKW60_05050"
     CDS             complement(7188..>7647)
                     /locus_tag="PKW60_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPJ98830.1"
                     /translation="SVQFPSFCADAHPVEARLSPWLCWGHELDGLFGIPLNVWPDSWR
                     LLNSARPEIWPASGGAGDSFLLYPAPGTLVPSLRLYRLRDGLEDIEYLRAAAEAGVSS
                     VVVRPESMAVCRWEQYAGNLPPKVLKATRDELLGLRTALGRAITETARSR"
BASE COUNT         1543 a         2257 c         2413 g         1434 t
ORIGIN      
        1 cgggcagcgt aatttcaaag gcagctccag ccatgtcggg gttaagcagc cggatttccc
       61 ccccgtgttt gcgcaccacg gcccggcaga ccgccagccc gaggccggtg ccggacaagc
      121 cttcccgaag gctgacgaaa ggctcgaaaa tgctctccgc gtgcttcgcg gagacgcccg
      181 gacccgaatc ctggaccatg atatgaacgc gagaacgatc ggaattggcc gaggtcttga
      241 ggagcagttg ccccccggtc tgactcatgg cctgttcgcc attcacgatg aggttcagca
      301 gagcctgttc aagctggtgt ttatcgacac ccacccgggg caggccctcc tcatactcct
      361 cggcgatggt gatgcctttt gcttcgagac ggtccgaaac cgtggtcacc agccggtgta
      421 agacataatt gaccaccgtg ggaacgggta cggagagccc gggacgcgca aatgccatca
      481 gatcattcac cagtttggtg agcgcgcggt tttgctccag gatggactca accgcactgg
      541 tctcttccgg gctgcgcatg cggcggagga gttgttgcgc ctggctggca acgatgttca
      601 gcgggcgttg cagggcatgg gaagcgccga tcgcaaattc ggccaggctg tcttcatctc
      661 ccgcgctcaa tgcgccggca ttgcgggcga tggcgtcggc gagatcctcg ctcagctcac
      721 gaagctcgcc gtacgcatga tgccgggcaa gcacggcgcc gcaggcgccc gcaaaggcca
      781 tcagtttaaa cgcatcccac gagtgatttt cgatcttctg cgactcggtt tcgaacataa
      841 tctggccgcg cgtggtgccg tcggccacca tggggagcat gagaatcccg tcgtgaagca
      901 cgggttcagt ccgttcagcg ccgcgccacc cgttttcgct cattcctata ccgagctcgt
      961 ccagggcttc cagcgcgagg agctcgacct catcaagctc ctgctgggtg gaaggagaca
     1021 gtggaatgcg aaactgctgc ggaggcgcgt cgggcttgtc ccaggtcatg ccgaccagat
     1081 ggcggttccc ttcgtcaaac gcgcaacaca tgccaggggc tgcatcgaga ccttcccgga
     1141 cggttgcgac gatgagcgtc aggacttcgc tgagggattg ggaaggccgg aggctggtgt
     1201 tcaaacggtt caaggcctgg agccacttga tctctttttt caaggaaagg gattccgcac
     1261 tttcggtgcg gccggggaga gtcacattgg atgcggcttt ctggagatcc ttaaatgcag
     1321 cggcgcagcc cgcagcgccg gattccagca ggacgaggcg ttgactcatc gcgtctcgag
     1381 tctgttcatg cagcgattcg atatccgcgt gctccaatcc gaggcgggcc gcatgcgcac
     1441 ggagatcgtg caggtgcgcc ggagaaggct tgtccccgcc gtccacgatc caggcactca
     1501 tggcattggc aaatccaacg atggcgacga gatggacgat gggccggtct ccgagcaggg
     1561 tgcctggggg caggtggtga aaccagatgg cctggctcaa atgccggggg agcgtccagc
     1621 gttccgccat ccatttgccc acgagcgcat gatcgacgtc aaacgcggcg cgttcgaggc
     1681 ggtccagttc agcgggctcg ttgttttcca gaaacgcgca atattcgtcc ggcatggccg
     1741 caatgagcgc caggcggcca acgtcatgga ggagcccggc cacataggct tctccggggg
     1801 agaccacgcg ggtgtgcgca gccgttttcg ctgcgcaggt tgcgcaggcc agagcgtgcc
     1861 gctgaaggcg ttcgaggtgc tgctggcagg attggacggg caggctggtc agggtaaccc
     1921 cgagcaccgc gcggcggaga ccttcgtaac ccacagcttg cacagcgtcc cgcagcagga
     1981 tggtttcaga agaggcgcgg ttcgcgccgg gtacgttggc cattcgcagc gcgcagctgg
     2041 ccagggccgg atcaatagcg aaaagggcgc cggcggcttc cagatccgga gaggcatcaa
     2101 acaagacgga caacgcccgc cccacaacct tcatggtggc cggcaggtgg tcaatgcctg
     2161 caatgaggtg tcttaactcg gattttccca tgctacaagc gccccgaagc agatgatagc
     2221 atgaaggccg ggcagaggcc agtccatgtc attggcgggg tcttgcatta agaccgtgga
     2281 accggaaggc cgcggaaatc atcacgtacg gggcgggttc acccgcgaac gggggctgcg
     2341 taaacggcgg gccaccagcc ctgcgaggcc cacgagaaat cccaggcaga gactgaagat
     2401 gccgcccaga tacatgccaa ccgccagttg gcccacgcgc agattccagc cgatcagggc
     2461 gcagcaggcg ccgatgacgc cgagggcgat gccggtaatc agaaaatcaa cgcggccgtc
     2521 actggaagga gtggatggcg gccgggcatc gcccccggca ccgccgggtt cggtaagcgc
     2581 gtcagcatcg cgatccatcg acttcaaaac cgcgatccgg actcggtagt ccacccaggc
     2641 gcgcagcaga tagcgcaagg cggcccctga cagcagggcc accgcaacca gaggaactac
     2701 cacgatgaga aggtctacca ttcgttgtcc tttctcgctt ctttgacgcc gtggcgagag
     2761 tccgggtttc aacattccgt gaaaagagtg cagactaccg ggtcgagcag gtatccttcc
     2821 aatacatcga gaagtcgagc gttcgcgccg cggccaaagc gaaacctgcc cgcggcccgt
     2881 gatgtctaac agggcgagat gaaagcgttt atcaagagtg tgcttctggc gggcggcatc
     2941 gcgctagcgc tcctgctctt caccacgcgc gtcctggcgc caaactttcc cagagtggtg
     3001 gggcgacccg ctatcgacca ggtgcgctct ttcacatggc cggggccgga acgcttttcc
     3061 ctggagaacc gcgacggaac cgtgcacata tggtctcaca gcgccgggga aatttccgta
     3121 aaagcgcgtg tccgggttta tgtgacggaa caggcccagg ctgcagaggc ggcagcttac
     3181 gcggaatccc tgtgcgccgc gagctctggc gcccgtgaaa tcaagatggt gacggaaccg
     3241 ggcgaacggc ctgatctgct cgaactccgg gtagattacg aagtcttcgt tccgcagggc
     3301 accgacatcg aggtcctgag cgtcaacggc aacgtgtatg tcgcctccgg ttgcggccgg
     3361 gtggcaatcc agggacgaaa cgcggatatt ttggtgagca aaccgagcgg agaagtgacc
     3421 gcgaagacaa ccaacgggcg gatcaacgtc gcggacgctc cggaaggcgc tcgtctggcg
     3481 accgtaaatg gcaatattta cgcgcacgtg tagggtggcg caattgacgc cgacacaaca
     3541 aacggggtca ttgtcgcgcg cattttgaat ggcggacagg ccggagcaga attgaagagc
     3601 atgaacgggg gcattacagt ggttctggac gagcagtttt ccgggacggt ggatgcgcgc
     3661 acgacccacg gcggaatccg ctctgatatt ccactggata cctcctcggg gatacagcgc
     3721 cggcatcatc tgcgcggaaa tcttggcttg gggcatgccc cgctcaacct gcagacgctt
     3781 aacggcaaca tctggctggc gaggggcagg caatgacgac agccatcact gcggcagcgg
     3841 ggtattggcc tggcatggat gcgatgcgcg ccacagagca atccgacgag acgctggtca
     3901 aacgtgccca ggcaggcgac accgaggcct tttcggagtt ggcccgccgc catgaactga
     3961 cggtgtataa cctgtcgctg cggtttatgc gcaacccttc cctggctgaa gatatggcgc
     4021 aggaagcctt cctcaaggca tatcagaaga tcgggaaatt ccggcgcgat tctcagtttt
     4081 cgacctggct ttaccgcatt gcgtgcaatg tatgcctgtc cgaattgcag aaacgtaaac
     4141 ggcgcggtga actgtctctg gcctatactc ccgagctctc cgggccgggc gctgcagcgc
     4201 cgcttcccca ggccgaagag gcagagctta tccgaaaatg tgttgccagg ctgcctcgcc
     4261 ggtatgcgga ggcgatcacc ttgttctacc tgcaggaatg ctcgtacgag gaggtcgctg
     4321 agatcatgga gattcccgta ggcacgctca aaacgtggct gcaccgcgcg cgcggccaat
     4381 tacgccgcat tgtacaacat gaattgaggt acgaagatga agagtgaaga tgggcccgcg
     4441 tcagacccct tgagccggcg catcgaggcg gcactgcgtc atgaaacgcc ggcgccgctg
     4501 cctggcgggt ttcacagtac ccttcaacgg cgccttcgcg tcgcggccat gctggagcgt
     4561 gaacggcaga ttttcctgca tcgcctcttg cacgtagccg tccccggcgt tctcggaatc
     4621 gcagtagcgg ctggcgtgat tctgcacggg gacgtttcac agaccttcct ctacgagttc
     4681 ccgggcttcc tgggccatgt tgactatctc accgcgctgg catcccggat gtggtggctt
     4741 tcagacaacc ccgcgctgct ggtctctttc ggggcgatag gcatgacgct tctgatagcg
     4801 ggcgcgtttg caggtatttt ccgctcaccg gcgggatcgc gccaacccgc gcagggaccg
     4861 attccctaag tagcgcaagg gcaaggggtt gctgccaaac gcaggttccg ggccggaaac
     4921 ctttgcatct gaggaatcat ctgttataat tccgctccgc tggaaaggga tttcccttgg
     4981 cgtcaaatca acgaggtctt ttgcaatgcc ggtcaaatac gaatgtccga aatgtggcag
     5041 gcgctttacg gagtggggcg ccgagaaagt cgggttcaag tgccccaaag atcaatggtg
     5101 ccccaaagac catccagacg atgtcgaact ggtgcgtgtc gggccatcgg aagaaggcgc
     5161 ttcgcggcgt ccaaccctga agcgtacgcc ccggaagctt gctcaggcaa aagcgccggt
     5221 catggacctc gaggaagacg aaggaatggt ccctgacatg gacgagcttg agggagacga
     5281 cgaattcgaa gacgaagacg atgagaccgg cgtgatcgac gatgccgagg aagaggaggc
     5341 aaccgttgaa gccgatgccc ccgacacagt cgccgtgatc gacgatgacg acgctccgga
     5401 cgatgccggg gcagacgaag atgtcgacga gaacgtggaa gatattatcg acgacgaaga
     5461 gctgccctga tccaggccag actgattgaa gatcaaacag gtgttataca tccgccccgg
     5521 tgccgggtta cggcggccgg ggcggattca gatcgggtga taccaaggtt ccctggagag
     5581 gcggacgcga cggggatcgc cgcgcggggc tgcactggcc gcgtttctcc ctgcgctact
     5641 cttcctgttg ccgcagtttc gccgctgctt ccctcgtggt gacgatgatg cggtccggtt
     5701 tgtacaggat gcctacgctc tgcatttcct tttgtttcag aaggtaattc tccaggaatt
     5761 ccgcgacgtt caccttcaca tcgtcattca gggtcacgtt gaccttgagt ccgcccgact
     5821 tactggcgag atcgcgcaga ggctggtcag cggcgatctg cagttgtgtg gcctgcttga
     5881 tgagattaag ggcttcgaac agatccttgc cattgagtcc gcgcacaagc tgtgtaatgt
     5941 ctgtgcgaag cttccccgcc aactgcgccg gcaccttcac gcgcgggagt atttcgacat
     6001 tcccctggtc atcgcgtgcc gtgccttccg ggagggcgtt ttccgtgcgc cgtggcgcac
     6061 ttgcggaagc gccgggagtt tttcctgcgc cgaattgtgt gcggggctca taatcatccc
     6121 acagaatctc tttcacgact tcgcgcggga gcgacaaggg cgccacccct tcgacgattt
     6181 caatctcgac atcggtggct gtccggcgca gcacctgcac gcgttcaaag cggcggccgt
     6241 cctggagaat cacggtatcg cccttgggtt cttgcgcagg ttcctcatac acggccgccg
     6301 gcgcactgga cgcggcgccg gattccgggg tgacgccagc ggccggggtt tccggagaaa
     6361 ccgcggatga cggggcggcg cctgtttcgg cctggtttcc agccgggctg gcggattcct
     6421 gggcaaagac cgcgggcaga accagcatca tcatagaaac gaacaacact gcatatcgca
     6481 tcatttgcga tcctttctgc agggcagccc ccgagtttaa cgttcctgat gctccctgtc
     6541 gcatgcggcc attctaggtt tgctcccgcc gttaagtcaa ggcgtacgat tacactttgt
     6601 gcggttacgc tgcctcgagc tgattgcgcg cgcctgcaat gccccggcat gcaggaagac
     6661 gatctgaagc gcgcatacca gcgaggcagc gggggctcat gccggcaaag gctggattgg
     6721 aggcaaaatg ctataatgcc gttttcaatg cggacaggag gacagcagga tggcactcat
     6781 tcttattgcc gacgatgatt cagcttcgtt tgatgtgctt gctgtagcac tgacggcaga
     6841 ggggcacaag gtcctttatg ccgccaacgg tcaggaggcg ctggaagcgg ccgtagaagc
     6901 ccagcctgac ctcatcttcc tcgatgtcat gatgccggtt tttgatgggt acgagacctg
     6961 tgaacgattg cgcaacgatc cgggcatccc gagcaaattg ccgatcattt tcctgaccgc
     7021 tacggatgag gatccgcgca aaattcagga agtcggcggc tccgattacc tcaccaaacg
     7081 tcacatgata gcggaacttc aggacatgct ggtgaaacat ctggggccca aagccttcgg
     7141 cgactgagcg ccctcagatg tctgcggggc gcgaggcccc cacggagtca gcgcgaccgg
     7201 gctgtttccg tgatagcacg gcccagggca gtacggagcc ccaacaactc atcccgggtg
     7261 gccttgagca ctttgggagg cagattcccg gcgtactgct cccagcggca cacagccata
     7321 ctttcaggcc gcacaacaac gctgctcaca ccggcctctg ccgcggcgcg gaggtattcg
     7381 atgtcctcca ggccgtcgcg cagccggtac agccgcagcg atggtacgag cgttcccggg
     7441 gcagggtaga gcagaaaaga gtcgccggcg ccgcccgaag ctggccagat ctccggcctc
     7501 gccgaattca gcagcctcca cgaatcgggc catacgttca aaggaatgcc gaaaaggcca
     7561 tcgagttcgt gcccccaaca gagccacggg ctcagccggg cttcaacggg atgggcatcg
     7621 gcgcaaaacg atggaaattg gactgaa
//
LOCUS       DAOPBQ010000108         7639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8706_length_7639_cov_4.168381, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000108 DAOPBQ010000000
VERSION     DAOPBQ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8706_length_7639_cov_4.168381"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /gene="pyrH"
                     /locus_tag="PKW60_05055"
     CDS             <1..552
                     /gene="pyrH"
                     /locus_tag="PKW60_05055"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015784746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPJ98831.1"
                     /translation="IYRGAEGVVQGPDRPTGDYMGMLATVINALALQAVLENKGVTTR
                     VMTAIEMRGVAEPYIRRRALRHLEKGRVVIFGAGTGNPFFTTDTAAALRANEIGAEIL
                     FKATKVDGVYDADPVKNPSATLYRELSYTEALSRKLRVMDATAISLCRENKLPILVFN
                     LTTPGSILKALQGEPIGTLVKGD"
     gene            556..1113
                     /gene="frr"
                     /locus_tag="PKW60_05060"
     CDS             556..1113
                     /gene="frr"
                     /locus_tag="PKW60_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPJ98832.1"
                     /translation="MPNELEKDAHNKMVKSVEAFARELSTIRTGRASTALLDSIEVEA
                     YGSRMKLNQMANVAAPEPRLLLITPWDKSQLGAIEKAILASPLDLTPGNDGHVIRIPI
                     PQLTEERRKDLVRLVGKLAEEARVAIRNVRRSAIEEIKKKQKDGDIPEDDAHKLTEQI
                     QKITDEHIEKVEETLKAKETEIMEV"
     gene            1237..1956
                     /gene="uppS"
                     /locus_tag="PKW60_05065"
     CDS             1237..1956
                     /gene="uppS"
                     /locus_tag="PKW60_05065"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPJ98833.1"
                     /translation="MDELDLTQLPRHIAIIMDGNGRWAARHGLSRAEGHESGAKSVRA
                     AIEACRELEGVDVLSLYAFSTENWRRSQAEIGALFSLLSKYIHRELENIHREGIRVRI
                     MGRREGLPDQVLKDLEYCEARTRDNRQMTLNVAINYGGRAEITDAARRIAREAAAGAL
                     RPGEVDEAAVAQRLYVPEIPDVDLLIRTSGEQRLSNFMLWQVSYAEIVTLGVLWPDFR
                     KRHLREAIARYQKRQRRFGGR"
     gene            1960..2790
                     /locus_tag="PKW60_05070"
     CDS             1960..2790
                     /locus_tag="PKW60_05070"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPJ98834.1"
                     /translation="MRKDGKLAVRSVTALIALAVFMTLIWTPGLRFLFTVFVGALSAV
                     GLYEYYAIVRARAISPETIGGILAGTCVTMSGHLGGAIVTNFALFGGCVLAASLHIVR
                     GQHSVAGLSSTVFGVFYIGWFAAHVPLMQSVPGVGPGLVMTLFVTVILTDTAAYFVGS
                     ALGKHKLAPKVSPNKTWEGAFAGLLAAMAGAAVLWYVKERSGLPSLPDWPVWRYVHAG
                     ALVSIVAQIGDLTESCLKRDAGVKDSGNFFPGHGGVLDRTDGFLFAAPVLYYLVTPLF
                     "
     gene            2837..3949
                     /gene="prfB"
                     /locus_tag="PKW60_05075"
     CDS             2837..3949
                     /gene="prfB"
                     /locus_tag="PKW60_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPJ98835.1"
                     /translation="MYEEEAELLAGYAERLRELRKCLNVDGTRAKISQFETEMAQPGF
                     WDDPEKAQKTVQQLKHHKQMIAAPDALQNELDDALVLVELGQSEADSSIGAEIAQLAQ
                     ALSKKLDDLELTSLFMDPRDSKPALVNIHPGAGGTESCDWASMLYRMIVRYCERREFN
                     VELADYIPGDEAGLKSATLRVEGPFAYGRLKSEEGVHRLVRISPYDANSRRHTSFAAI
                     EIVPELEEETDIEIKEEELKMDVFRSSGPGGQKVNKTSSAVRLTHLPTGIVVACQIER
                     SQHRNRETALQMLKAKLYDIELKKREEEAMAHREGQQDVAWGSQIRSYVLHPYQMVKD
                     HRTEVQIGNIDAVLDGDLDELVEAFLRWNLKRHKNN"
     gene            3972..5540
                     /gene="lysS"
                     /locus_tag="PKW60_05080"
     CDS             3972..5540
                     /gene="lysS"
                     /locus_tag="PKW60_05080"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPJ98836.1"
                     /translation="MTEEHHSTEQQLRENRIEKMARFRERGDEPFKYSFARTHSIAEA
                     RAAFLEAEAASDEPEQVSVETAVAGRMVALRGQGKVTFADLRDESDRIQIFFRGAELG
                     EAAYEQLKDLDIGDFLGIEGAVFRTRRGEVTVRATKYTLLSKSLRPLPEKWHGLTDVE
                     TRYRQRYLDMVANPEVLETFRRRSAIIATLRQWLTARGFLEVETPMLHYIPGGATARP
                     FITHHNTFDRDFYLRIAPELHLKRLLVGGFEKVFEINRSFRNEGVDTLHNPEFTMMEL
                     YEAYQDYLGLMDETEEMLAHVAREITGSTTFDYQGQTVDMTRPWKRISLFDAIREYAG
                     IDLEPHRNDRNAAAELAKQAGCVVEDSMGFGKIVDEVMSEKVKPHLRQPTFLYDYPVE
                     LSPLAKKKRGAPHLTERFQLFVAGMEMTNAFSELNDPIDQRERFMEQVRLRERGDEEA
                     QHLDEDYLVALEVGMPPAAGLGIGIDRLTMLLTNQASIREVILFPQLRVIEATAKLEL
                     PHPSVDAKGTVETS"
     gene            5596..6867
                     /locus_tag="PKW60_05085"
     CDS             5596..6867
                     /locus_tag="PKW60_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HPJ98837.1"
                     /translation="MRFEAFIASRYLRGKRKSRFVSLITLISVAGVSVGVMALIVVMS
                     VMTGFDNELRATIIGNRAHLTVMERYRDEIADPDAAEARIRALCPEVQGASPFIEVLS
                     LLKTLRGPDGSQLVEFAYVVGVDPEKASGVTQLAENLTDANGRTHGAGTLPEYKEIVL
                     GYVLASKLGAYLETPLIIVTPQMKSGTYMTHPTEMLVKVSGLSQAQMHEFDTVYGFIT
                     LETAKKLTGKDGVDGIQLRLDDPFKAKEISNRIETEMPEFTAITWYESQQAFFDALKE
                     EKVVMFIILLFIILVAAFNITSTLIMVVMEKRRDIGILRTIGVSSGSVLRLFVLEGLY
                     IGLSGTFVGLILGIVLSHYLNAVASFIAWMMGVDLFNSQIYYFDRIPVAIQWMDVGWV
                     TVCAVLLTFLSTIYPAWSASRLDPVDALRYE"
     gene            6860..7555
                     /locus_tag="PKW60_05090"
     CDS             6860..7555
                     /locus_tag="PKW60_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ98838.1"
                     /translation="MSNITSIPVLECRHLEKTYNDGSRELRILQGIDLEVHDGDLMVI
                     TGPSGVGKSTLLHIIGTLDRPSSGEVLFRGTPLQKTDRAGINRIRNEEIGFVFQFYHL
                     LPEFTALENVMMPALSRGKRRSACQPRAEELLRTVGLAERMTHKPGQLSGGEQQRVAI
                     ARALFNEPSVVLADEPTGNLDENTGRGILELLYSLNAERRVTLVMVTHDEGIAERAVR
                     WIHLHEGRAIVKK"
BASE COUNT         1607 a         2114 c         2560 g         1358 t
ORIGIN      
        1 atataccgcg gcgccgaagg cgtggtgcag ggtcctgacc ggccgacggg tgattacatg
       61 gggatgctgg cgacggtcat caacgcgctg gcgctccagg cggtgctcga aaacaaaggg
      121 gtgacgacgc gcgtcatgac ggccatcgag atgcgcgggg tggcggaacc gtatatccgg
      181 cgccgggccc tgcgtcacct ggagaagggc cgggtggtca ttttcggcgc ggggacaggc
      241 aacccgtttt tcacgacgga tacggcggcg gcgctgcgcg cgaacgagat tggcgcggag
      301 atcctcttca aggccaccaa agtggacggg gtgtacgacg ccgatccggt gaagaacccc
      361 tcggccacgc tgtaccgtga gctctcgtac acggaggcgc tctcgcggaa gcttcgtgtg
      421 atggatgcaa cagcgatctc gctgtgccgt gaaaacaagc ttccgatcct ggttttcaat
      481 ttgaccacgc cgggcagtat tctgaaagcc ctgcaaggag agcccatagg caccctggta
      541 aaaggagact gagatatgcc aaacgagctc gagaaagacg cccacaacaa aatggtcaag
      601 agcgtggagg ccttcgcgcg cgagttatcg acgattcgaa ccggccgggc ctccaccgcg
      661 ttgctggatt caatcgaggt cgaggcatac gggtcccgga tgaagttgaa ccagatggcg
      721 aacgtggcgg cgccggagcc gcggctcttg ctgattaccc cctgggataa atcgcagctg
      781 ggggccattg aaaaagcgat tctggcgtct ccgctggact tgacgcccgg caatgacggg
      841 cacgtcatcc gcattcccat cccgcagctc acggaggagc ggcgcaagga cctcgtgcgg
      901 ctggtgggca aactcgccga agaagcgcgg gtggcgattc gcaacgtgcg ccgctcggcc
      961 atcgaagaaa tcaagaagaa gcagaaagac ggcgatatcc ccgaggacga cgcgcacaaa
     1021 ctcacggaac agatccagaa gatcacggac gagcatatcg agaaggtcga agagacgctg
     1081 aaggccaaag aaaccgagat catggaagtg taatcgcatg cgccggcacc acaactgaat
     1141 ggccgagttc cgcggagaat tggggcgccc catctctccg cggttttgtt ttggacggtg
     1201 tgcgggcacg atgaacgggg agcgcctgga tgagcgatgg atgaactgga cctgacacag
     1261 ctgccacggc atatcgccat catcatggat ggcaatggcc gctgggccgc cagacacggc
     1321 ctgtcccgcg cggaagggca cgaatcgggc gcaaagagtg tgcgtgccgc gattgaggcg
     1381 tgccgcgaac tcgagggcgt ggacgtgctg tcgctgtatg ccttctcgac ggagaactgg
     1441 cgccgctccc aggcggagat cggtgcgctg ttcagcctgt tgagcaaata catccaccgg
     1501 gagctggaaa acatccaccg ggagggcatc cgggtgcgga tcatgggccg tcgcgaagga
     1561 ttgccggacc aggtgctgaa ggatttggag tattgcgagg cgcggacgcg cgacaaccgc
     1621 cagatgacgc tcaacgtggc catcaactac ggggggcggg cggagattac ggatgcggcg
     1681 cgccggattg cccgggaagc agcggcgggc gccttgcgcc ccggcgaggt ggatgaggcg
     1741 gcggttgcgc agcgcttgta cgtgcccgag atccccgacg tggacctgct gatccggacg
     1801 agcggggaac agcgcctgag caacttcatg ctgtggcagg tgtcgtatgc ggagatcgtg
     1861 accctgggcg tgttgtggcc ggattttcgg aaacggcatc ttcgcgaggc gatcgcccgc
     1921 taccagaagc gccagcgccg ctttggggga cggtaggcca tgcgcaaaga cggcaagctg
     1981 gcagttcgca gcgtgacggc gctcatcgcg ctggccgtgt tcatgacctt gatctggaca
     2041 ccggggctgc gttttctgtt cacggtgttt gtgggcgcct tgagcgccgt cgggttgtac
     2101 gaatattacg cgatcgtgcg ggcacgggcg atctcgcccg agacgatcgg cgggatcctg
     2161 gcgggcacct gcgtgaccat gagcggacac ctgggcggcg cgattgtgac gaattttgcg
     2221 ctgtttggcg gctgtgttct ggcggcctcg ctgcacatcg tccgggggca gcactcggtg
     2281 gcgggccttt cgagcacggt attcggcgta ttctatatcg gctggttcgc cgcgcatgtg
     2341 ccgttgatgc aaagcgtccc gggagtgggc ccgggcctgg tgatgacgct gtttgtgacc
     2401 gtgatcctga cggatacggc ggcgtatttc gtggggtcgg cgctcgggaa gcacaaactg
     2461 gccccgaagg tcagcccgaa caagacctgg gaaggcgcgt tcgcggggtt gctcgcggcg
     2521 atggcgggtg cggcggtgct gtggtatgtg aaagagcgct ccgggctgcc gtcgctgccg
     2581 gattggccgg tctggcgtta tgtgcatgcg ggcgcgctgg tatctatcgt ggcccagata
     2641 ggcgatttga cggaatcgtg cctgaaacgc gacgcaggcg tgaaagactc gggcaacttt
     2701 tttccgggcc acggcggtgt tctggaccgt accgacgggt ttctgttcgc cgcaccggtc
     2761 ttatattatc tggtcacgcc attattctga cgcggaagcg ccagatgagc tcggcagcgc
     2821 ataaagggtt cgggaaatgt acgaagaaga ggcagaactt ctggcgggat atgcggagcg
     2881 tttgcgcgaa cttcgcaaat gcctgaatgt agacggcacg cgggcgaaaa tttcgcaatt
     2941 cgaaacggag atggcacagc ccggtttctg ggacgatcct gagaaggcgc agaagacggt
     3001 gcagcagctc aagcaccaca aacagatgat cgccgcaccc gacgccctgc aaaacgagct
     3061 ggacgatgcg ctggtgctgg tggaattggg ccagtcggaa gcggattctt caatcggggc
     3121 ggagatcgcg cagctggcac aggcgctttc gaaaaaactg gatgacctgg agctgaccag
     3181 cctgttcatg gacccgcggg actccaaacc cgcgctggtg aacatacatc ccggggcggg
     3241 cggcacggag tcgtgcgact gggcctcgat gctgtaccgg atgatcgtgc ggtattgcga
     3301 gcggcgggaa ttcaacgtgg aactggccga ttacatcccg ggcgacgagg ccgggctcaa
     3361 gagcgccacg ctgcgcgtgg aagggccgtt tgcctacggc cgcctcaagt cggaggaagg
     3421 cgtacaccgg ctggtgcgga tctcgccgta cgacgcgaac agccggcggc acacttcctt
     3481 tgcggcgatt gaaattgtcc cggaactgga agaagaaacg gacatcgaga tcaaagaaga
     3541 agagttgaaa atggacgtct tccggtcgag cggtccaggc ggccagaagg tcaacaagac
     3601 gagcagtgcg gtgcgcctga ctcatcttcc caccgggatc gtggtagcct gccaaattga
     3661 acgttcccag caccgcaacc gggaaaccgc cctgcagatg ctgaaagcga agctgtacga
     3721 catcgaattg aagaaaagag aagaagaggc gatggcccat cgcgaaggcc agcaggacgt
     3781 cgcctgggga agccagattc gcagctacgt gctgcatcca tatcagatgg tgaaagacca
     3841 ccggacggaa gtccagatcg gcaatattga cgccgtgctg gacggcgatc tcgacgaact
     3901 ggtggaggcg tttctgagat ggaacctgaa acgccacaaa aacaactaac cgatgagccg
     3961 gggaaagaac catgaccgag gagcaccatt caaccgaaca gcaactgcgg gaaaaccgca
     4021 tcgaaaagat ggcccgattc cgcgaacgcg gcgacgagcc gttcaaatac agctttgccc
     4081 ggacccattc catcgcggag gcccgggcgg cgttcctgga agcggaggcc gcttcggatg
     4141 agccagagca ggtcagcgtt gaaacggctg tggcagggcg catggtggcg ctgcggggcc
     4201 agggcaaagt gacgtttgcc gatctgcggg acgaatcgga ccggatccag atttttttcc
     4261 gcggcgcgga gctgggcgag gctgcgtatg agcaattgaa agatctggac atcggcgatt
     4321 ttctgggcat tgaaggggcg gtgttccgca cgcggcgggg cgaagtgacg gtccgcgcga
     4381 cgaaatacac gctgctgtcg aaatcgctgc gcccgctccc ggaaaaatgg cacggtttga
     4441 cggatgtgga gacgcgctac cgccagcggt atttggatat ggtggcaaat ccggaggtgc
     4501 ttgagacctt tcgccgccgg agcgcaatca tcgccacatt gcggcagtgg ttgacggcgc
     4561 ggggctttct ggaagtcgag acccccatgc tgcactacat tcccgggggc gcgaccgccc
     4621 ggccgttcat cacgcaccac aacacctttg atcgggactt ttatcttcgg atcgcgccgg
     4681 aactgcatct caagcgcctg ctggtcggcg gttttgagaa agtcttcgag atcaaccgga
     4741 gtttccgcaa tgagggcgtg gacacgcttc acaatcccga gttcaccatg atggaactct
     4801 acgaggccta ccaggattac cttggattga tggacgagac tgaggaaatg ctggcgcacg
     4861 tggcccgcga gatcacggga agcacgacct tcgactacca ggggcagacc gtcgacatga
     4921 cgcgtccctg gaagcgcatc tccttatttg atgccattcg cgaatatgcg gggatcgacc
     4981 tggaaccgca ccggaacgac cggaatgccg cggcagaact ggcgaaacaa gccgggtgcg
     5041 tggtcgagga ttccatggga ttcggaaaga tcgtggacga ggtcatgtcg gaaaaggtga
     5101 agccgcacct gcgtcaaccc acctttctgt atgattaccc ggtggagctt tcgccgctgg
     5161 ccaagaagaa acggggcgcc ccgcacctga cggaacggtt ccaactcttc gtcgcgggca
     5221 tggaaatgac caacgcattc agcgagctca acgaccccat cgatcagcgt gagcgattca
     5281 tggagcaggt ccggctgcgc gaacggggcg acgaggaagc ccagcatctc gacgaagact
     5341 acctcgtggc gctcgaagtc gggatgccgc cggcggcggg tctgggcatc gggatcgacc
     5401 gcctgaccat gctgctgacg aatcaggcgt cgatccgtga ggtaattcta ttcccgcagc
     5461 tgcgggtcat tgaggctaca gccaaactgg aactgccgca tccgtctgta gacgccaagg
     5521 gtacggtaga aacgagttga agatgcggca gccaatgcgg ctgctgcgcg cagaatatgc
     5581 agaaagacgt cctacgtgcg gtttgaagcg ttcatagcga gccggtacct gcggggcaaa
     5641 cgcaagagcc ggtttgtgag cctgatcacg ctgatctcgg tggcgggcgt gagcgtgggc
     5701 gtcatggcgc tgattgtcgt gatgagcgtg atgaccggat tcgataacga actccgcgcg
     5761 accatcatcg gcaaccgcgc ccacctcacc gtgatggaaa ggtatcggga cgagattgcc
     5821 gatccggacg cggccgaggc ccggatccgg gcgctctgcc ccgaagtgca gggggcgagc
     5881 ccgttcatcg aggtgctgtc gctcctgaag acactgcgcg gcccggacgg ctcccagctc
     5941 gtcgaatttg cctacgtggt cggggtggac ccggaaaaag cgagcggcgt aacgcagctg
     6001 gcggagaatc tcacggatgc gaacggccgc actcacggtg cggggaccct gcccgaatac
     6061 aaggagatcg tgctgggcta tgtgctcgcg agcaagctgg gcgcctacct ggagaccccg
     6121 ctgatcatcg tcaccccgca gatgaaaagc ggcacataca tgacccatcc gaccgaaatg
     6181 ctggtcaaag tgagcgggct gtcgcaggca cagatgcacg aattcgacac cgtgtacggc
     6241 ttcattacgc tggaaacggc gaaaaagctg accggcaaag atggcgtaga cgggatccag
     6301 ctgcgcctcg acgatccatt caaggcaaag gagatttcga accggatcga gacagagatg
     6361 ccggaattca cggcgatcac gtggtatgaa agccagcagg cctttttcga cgcgctgaag
     6421 gaagagaaag tggtgatgtt cattatcctt ctgttcatca tcctggtcgc ggcatttaac
     6481 atcacaagca cgctcatcat ggtggtgatg gagaaacggc gggatatcgg catcctgcgc
     6541 acgataggcg tgagcagcgg gtccgtgctc cggctctttg tcctcgaagg gctgtatatc
     6601 gggctgtcgg gtacgttcgt cggcctgatc cttggaatcg tgctctcgca ctacctcaac
     6661 gccgtggcgt cgttcatagc gtggatgatg ggcgtggacc tgtttaactc gcagatctac
     6721 tattttgacc gtattccggt ggccatccaa tggatggatg tgggctgggt gaccgtctgc
     6781 gcggtgctat tgacgttttt gtcgacgatt tatccggcgt ggagtgcatc gcgccttgac
     6841 cccgtggatg ccttgcggta tgagtaacat cacgtccata ccggtccttg aatgccgcca
     6901 cctcgaaaag acctataacg acggcagccg cgagctgcgc atcctgcagg gcatagacct
     6961 ggaagtgcac gacggcgacc tgatggtcat tacggggccg tcgggagtag gcaagagcac
     7021 gctgcttcac atcattggca cgctggaccg gccctcgtcg ggcgaggtgc tgttccgggg
     7081 aacacccttg cagaagacgg accgggccgg catcaaccgc atccgcaacg aagagatcgg
     7141 gtttgtgttc cagttctacc acctcctgcc ggaattcacc gcgcttgaaa acgtgatgat
     7201 gccggcgctg agccgtggaa aacggcgctc ggcatgccag ccgcgcgccg aagaactgct
     7261 caggacggtc gggttggcgg aacgcatgac gcacaaaccg gggcagctca gcggaggcga
     7321 gcagcagcgt gtggccatcg cccgggccct gtttaacgaa cccagcgtgg tgctggctga
     7381 cgaacccacc ggaaacctcg acgaaaacac cggcaggggc atcctggagc tgttgtactc
     7441 gctgaatgcg gagcgccggg tcacattggt gatggtgaca catgacgaag ggattgctga
     7501 acgcgcggtg cggtggatcc atctgcatga agggcgggcc atcgtaaaga aatgacttcc
     7561 gaggagggtc cggccatgga ttgcactcgg cggcagttcc tctcatcggc agcggggact
     7621 gccgcgggcc tgacgctgc
//
LOCUS       DAOPBQ010000109         7592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8765_length_7592_cov_4.578612, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000109 DAOPBQ010000000
VERSION     DAOPBQ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8765_length_7592_cov_4.578612"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="PKW60_05095"
     CDS             complement(<1..1148)
                     /locus_tag="PKW60_05095"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HPJ98839.1"
                     /translation="MATINQNYGKLEAGYLFPEIGRRTREFLAAHPGASVMRLGIGNT
                     TEPLPPSIIDGLRRGVDNLAKAETYTGYGDEQGNSELREALAARYAAYGVTLEPDEFF
                     VSDGAKPDSANIQSIFGTDNVVAVQDPAYPVYVDSNVIAGRTGRAVNGQYDGFLYMPC
                     TRENGFFPEVPSQKTDLIYICSPNNPTGTVATREQLAGFVSYALAHKAVLIFDAAYAA
                     FIADPALPRTIYEIDGARDCAIEINSFSKEAGFTGVRLGWSVVPKTLVCENGGPGALH
                     RLWFRRQTTMFNGASNIVQEGGLAVLSEAGQRECRAIIDYYMANAKVIREGLESIGLE
                     VFGGVNAPYIWMATPGGMPSWEFFDKLLHEAHVVGTPGSGFGPSGEGY"
     gene            1551..2552
                     /locus_tag="PKW60_05100"
     CDS             1551..2552
                     /locus_tag="PKW60_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPJ98840.1"
                     /translation="MNSYERYMGMVKGEKVDFVPRVPILMHFAARYAGVTYGDFARDA
                     ATLVAVNRKLIEDFGFEQLDIMSDPWRETVDFGGTIEYLDDAVPKCTRHPLADSRDLG
                     MLLQPDPQSSERMSNALRALALYKDFGYKQYSITGWVEGPAAEAATLRGVANFLMDLM
                     TEEEFAGNLMDRCVVNAVAFAQAQLEKGADTIGIGDAIASQLSPELYGRFVFPREKQL
                     IDAIHAAGGLVRLHICGDINHLLSQIALLNVDILDCDWMVDMAAARKILGSRVVLTGN
                     LDPVQAVMEATPQAIRDTLRAIYDTVGNPYFVNAGCEIPAATPLENLRALCEPIPAA"
     gene            2606..3637
                     /locus_tag="PKW60_05105"
     CDS             2606..3637
                     /locus_tag="PKW60_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98841.1"
                     /translation="MITALWITCAWMLAAEPAGQLAFVAGTEQEDRRVELVDLSTGVL
                     TSVGPGARDGAPAWSPDGQWLAFPTARPGGTGIRVVRADGAEARELPHAHPWNDMPRW
                     SPDSKLLAYTGSRDTSGIRPESLAAMASVMVYDLEANTEEQWGEETMSLFRPVWMNAG
                     TVIAIGVIPGEKRLTTDIFIVTPSTVSPLPAAAMPSAGQYVEWAAEVNLRENLLAYES
                     NDGGDREVFVLSFKRGSTDVSNHRAADWNPVWQPNGRWLAFESFRSGRRGVYRVLPGT
                     INVIPVAVAEDSDNWHPAWSPDGAFLAYVSTREGMPQLFVTELESGKVRQVTTGARFA
                     LAPAWRPVP"
     gene            3634..4644
                     /locus_tag="PKW60_05110"
     CDS             3634..4644
                     /locus_tag="PKW60_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ98842.1"
                     /translation="MRRLSWLWPMMLLCATAGARNTDGVLDLIITPNNGVPAIIAPGE
                     SFEATLTRRADLRLAGMEGELLALEPDWTALPGGMHRAICTTPEMLATGTYALEAVAD
                     GRTDTNIRAVFVTRPMQDYPVIHLTDVHIGKANRDPAPETVFRNLVEHINGIHAAFVL
                     ITGDLTEQGRPEEFRTFLDVLDTCVLPTFVCAGNHDRRALNYEETFGPLVYSFRYGPD
                     GYLVFDTKDIIIADPLDTQMGELQRLRRAIKPSRWSIGASHRYDPSMSMRSQLVLFVD
                     DPLDYLLFGHWHRESQEKRVPWGTTPVVVTPAALDGAWRLILVAQTGIFPQSFQQISA
                     GK"
     gene            4747..5853
                     /locus_tag="PKW60_05115"
     CDS             4747..5853
                     /locus_tag="PKW60_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HPJ98843.1"
                     /translation="MPAPRQFQLLIKPASADCNLRCDYCFYLRAEELYPGPARHVMPD
                     DVQEQMILNLLEYRFPQTVFAWQGGEPTLCGLDFFRRTVELQQKYGAPGQSVGNALQT
                     NGILLDAEWCRFFREYQFLIGLSLDGPQDVHDRFRVNMSGRGSWDQVMRAAELMNRHR
                     VEFNILCVVNGQNVEMGADLLRWYIDQGFSYLQFIPCVEAGYGHSVPPEKFGKFLCDI
                     FDFWAKDGFGRVSIRDFDAMLAQRMGQGQPMCTFGRVCNHYVVVEHNGDVYPCDFFVY
                     DHWKLGNLMDAPLHTFLESETYRKFAYQKSKVPACAGCAWRPWCHGGCQKDRLLQGGD
                     FRQPTVLCEAYKMFFAHAAPRLNALAKKMARSRG"
     gene            complement(5881..7149)
                     /locus_tag="PKW60_05120"
     CDS             complement(5881..7149)
                     /locus_tag="PKW60_05120"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPJ98844.1"
                     /translation="MNAREYLASLEFHGVKLGLENIRHLLACAGNPQTRYPCVHVAGT
                     NGKGSVLAMVDAMCRAAGMRTGRYTSPHIIDVRERFLIDARPVDDAALDASLAYFRRI
                     AEQMTPPPTYFELVTAAAFHLFQQHEVDLALIEVGLGGRFDATNVITPVAAAITNIDL
                     EHTQYLGDTLEKIAFEKAGILKESVPAVIGETRPGPRDVILARAGELGCPVRLMERDF
                     RFEMQGHSANPAFTYRSERMTLGPVTLGLPGAFQTFNAALAAALAECLAQTFPQLDSR
                     AMLAGLRTARWPCRLERVLDTPPVIIDIAHNPAGIRQLVRQVPQCAAILAVSSDKNAA
                     EMVRYLATTAEPLILTQFAGHRALPVEQLCAAAGNAAYQRVDCIDEAICAGMRAATAD
                     RPLLITGSVYTAGQARALLVKEYNARPLEF"
     gene            complement(7146..>7592)
                     /locus_tag="PKW60_05125"
     CDS             complement(7146..>7592)
                     /locus_tag="PKW60_05125"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HPJ98845.1"
                     /translation="SMGSVVGEKFCRAADDAIRERVPLVSFATSGGARMQEGTLGLMQ
                     MAKTADAVRALNEAAIPYISVLTNPTTGGVYASFASLGDVVLAEPGAEIGFAGKRLIE
                     GALKVHIPEGFQTSEYQFANGFVDAIVPRTEMRPMLARLLLYLKPQ"
BASE COUNT         1376 a         2347 c         2443 g         1426 t
ORIGIN      
        1 aaatagcctt caccgctggg cccgaaaccc gaccccggag tgccgaccac atgggcctcg
       61 tggagcagtt tgtcgaaaaa ttcccaggaa ggcatgcctc cgggcgtagc catccagata
      121 tagggcgcgt tcacgccgcc gaacacttcg agcccgatgc tttccaggcc ttcgcggatc
      181 acttttgcat tggccatata gtagtcgatg atcgcgcggc attcgcgctg gccagcctcc
      241 gaaagcactg ccaatccgcc ttcctgcacg atattcgacg cgccgttgaa catcgtggtc
      301 tggcggcgga accacagacg gtgcagggcg cccgggccgc cgttttcgca caccagtgtc
      361 ttgggcacca cggaccatcc caggcgcacc cctgtgaatc cggcctcttt cgaaaagctg
      421 ttgatctcga tcgcgcagtc tcgggcgccg tcgatctcgt agatggtccg cggcagagcc
      481 gggtcggcga tgaacgccgc atacgccgcg tcgaagatga ggaccgcctt gtgtgccaag
      541 gcataggaga cgaaaccggc cagttgttcc cgcgttgcga ccgtgccggt aggattattg
      601 gggctgcaga tgtagatgag gtccgttttt tgcgaaggca cctccgggaa aaatccgttt
      661 tcccgggtgc agggcatgta gagaaatccg tcgtactggc cgttgaccgc ccggcccgtg
      721 cggcctgcga tgacattgct gtcgacatac accggatagg cgggatcctg caccgcgacg
      781 acgttgtcag tgccgaagat ggactggatg ttcgcggaat cgggtttcgc gccgtcgctg
      841 acgaagaatt cgtccggttc cagagtcacg ccgtaggcgg catagcgggc ggcgagcgcc
      901 tcgcggagtt cgctgttgcc ctgttcatcg ccgtagccgg tataggtctc ggctttcgcc
      961 agattgtcga cgccccgccg caatccgtcg atgatcgacg gtgggagagg ttcggtcgtg
     1021 ttgccgatgc ccagacgcat gaccgaggcg cccggatggg cggccaggaa ttcgcgtgtg
     1081 cgccggccta tttccggaaa gaggtagcct gcctcgagtt tgccgtaatt ctgattgatg
     1141 gttgccattc ggttacattt ctccgcgtag tgaattttat gcctgtctgg aatcggtttc
     1201 gctgtccgtt aacgccactc ccctcgggcg cattccggca cggcgatctc tggcgcgccg
     1261 gggcgcggaa cgcaatgccc gcgccggaca gcctccattt caagtgtaat atattaccgc
     1321 ccggaaatgc gagaccgggc gaaccgccgg tttcttacag ggttgcgcgg attggatggg
     1381 ctcgagggcg ttaaagaccg gtttgccatg cagttagctg ggtttccggc agtaaaatcc
     1441 ggtcttattc gcttcaattc gcagcccggg agcaggtgaa aacggttgcg cggtgatgag
     1501 aacggcgtgt atggttcacg cacaggcaag gcgcgagaaa gggcactcgg atgaacagtt
     1561 acgaacgcta tatggggatg gtgaaaggcg aaaaggtgga ttttgtgccg cgtgtgccca
     1621 tcctgatgca ttttgccgcg cggtatgcgg gagtgacgta cggcgatttc gcgcgcgacg
     1681 ccgcgacgct cgtggcggtc aacagaaaac tgatcgaaga tttcgggttc gagcagcttg
     1741 atatcatgtc ggatccctgg cgtgaaaccg ttgatttcgg tgggacgata gaatatctgg
     1801 atgatgccgt gcccaaatgc acccgccatc cgctggcgga ttcccgcgat ctgggcatgc
     1861 tcctgcagcc tgatccgcaa agctccgagc ggatgagcaa cgccctgcgc gcgctcgccc
     1921 tctacaagga tttcggatac aaacagtatt cgatcacggg ctgggtcgaa gggccggccg
     1981 ctgaagccgc cacgcttcgc ggcgtggcca actttctcat ggacctgatg accgaggaag
     2041 aattcgccgg caacctgatg gatcgatgcg ttgtcaacgc ggttgccttc gcacaggcgc
     2101 agctcgaaaa aggcgccgac accatcggca tcggcgacgc catcgcgagc cagctgtcgc
     2161 ctgaactcta cgggcggttc gtgtttcccc gcgaaaagca gctcatcgat gccatccacg
     2221 ccgccggcgg gctggtgcgc ctgcacatct gcggcgacat caatcatctg ctctctcaga
     2281 tcgccctgct gaacgtcgac atcctcgatt gcgactggat ggtggacatg gccgccgccc
     2341 gcaagatcct cggcagcagg gttgtactta ccggcaacct ggacccggtt caggcggtga
     2401 tggaagccac gcctcaggcc attcgcgaca ccctgcgcgc catctacgac actgtgggca
     2461 acccgtactt cgtgaatgcg ggctgtgaaa tacccgcggc caccccgctc gaaaaccttc
     2521 gcgccctctg tgaacccatt cccgcggcgt gaacaggagg cagccgtaac gcgcgcatct
     2581 cagccgatgt gttacaattc gcgccgtgat taccgcactc tggataacct gcgcgtggat
     2641 gctggccgcg gagcccgccg gccagctcgc gtttgtagcc ggcaccgagc aggaagaccg
     2701 gcgcgtcgaa ctcgtggacc tcagcacggg cgtgctgacg tccgtgggcc cgggcgcccg
     2761 cgacggggcc ccggcctggt cgcccgacgg ccagtggctg gcctttccta ccgcccggcc
     2821 cggaggcacg ggcatacggg tggtccgcgc ggacggcgcc gaagcgcgcg agcttcccca
     2881 cgcgcacccc tggaacgaca tgccccgctg gtcgccagac agcaagttgc tcgcgtacac
     2941 cggctccagg gatacgagcg gcatccgccc tgaatcgctc gccgcaatgg cctccgtcat
     3001 ggtctacgac ctggaggcga acacggaaga gcaatggggc gaggaaacca tgagcctgtt
     3061 ccgtcccgta tggatgaacg ccgggaccgt aattgccatc ggcgtgattc ccggagagaa
     3121 acgcttgacg acggacattt tcattgtgac gccaagcacc gtgagcccgc ttccagccgc
     3181 cgccatgccg tccgcaggcc agtatgtcga atgggccgcg gaggtcaatc tccgggaaaa
     3241 tctgctcgcg tacgaatcca acgatggcgg cgaccgcgag gtgtttgttc tgtcgtttaa
     3301 acgcggctcg acggacgtat caaaccaccg ggccgcggat tggaatccgg tatggcagcc
     3361 caacgggcgg tggctggcgt ttgagtcgtt tcggtcgggg cgccggggcg tgtaccgcgt
     3421 gcttcccgga accatcaacg tcatccctgt cgcggtcgca gaggacagcg acaactggca
     3481 tcccgcctgg tcgcccgacg gcgcgttcct cgcctatgtc tccacgcgcg aaggcatgcc
     3541 ccagctgttc gtgacggagc ttgagagcgg caaggtccgc caggttacca caggcgcccg
     3601 tttcgccctg gcgcccgcct ggaggcccgt gccatgagac gcctgagctg gctctggccg
     3661 atgatgctgc tgtgtgcgac agcgggcgcg cgcaatacgg acggcgtgct ggacctcatc
     3721 atcaccccca ataacggagt ccccgcaatc atcgctcccg gcgaatcctt tgaagccacg
     3781 ctcacgcgcc gcgccgacct gcgcctcgcc ggcatggaag gtgaacttct ggccctcgag
     3841 ccggactgga cggcgttgcc gggggggatg caccgggcga tctgcacgac gcccgagatg
     3901 cttgccaccg ggacgtatgc cctggaggcg gttgcagacg ggcgcaccga caccaacatc
     3961 cgggcggtgt ttgtcacgcg ccccatgcag gactatccgg taatccatct caccgatgta
     4021 cacatcggca aagcaaaccg cgaccccgcc cccgaaaccg tgttccgcaa tcttgtagag
     4081 cacatcaacg ggatccatgc cgcatttgtg ctgattaccg gcgatctcac cgagcagggg
     4141 cgcccggagg agttccgcac gtttctcgac gtgcttgata cctgcgttct ccccacattc
     4201 gtctgcgcgg gcaaccacga tcgccgcgcc ctgaactatg aggaaacctt cgggccgctg
     4261 gtctacagct ttcgttacgg acccgatggg tatctggttt tcgacacaaa agatatcatt
     4321 attgcggatc ccctcgacac acagatgggc gagctccagc ggctgcggcg cgccatcaag
     4381 ccgtcgcgct ggtcgattgg cgcgagccac cgttacgatc cgtccatgag catgcgcagc
     4441 cagttggtgc tctttgtcga cgaccccctc gactacctcc tgttcggcca ctggcaccga
     4501 gagagtcagg aaaagcgtgt gccgtggggt acgaccccgg tcgtggtcac accggccgct
     4561 cttgacgggg cgtggcgtct cattctggtc gcgcagaccg gcatcttccc ccaaagtttt
     4621 cagcagatca gcgccgggaa atgagtcccg ccgggacgct ccggcacgcc ctgtcaccgg
     4681 ctctgcagaa ccagattcca catcgaattt tggcgcggga gatcgccaat ttgatacact
     4741 cgcggcatgc ctgcgccacg acagtttcaa ttgctgatca agcccgcgag cgccgactgc
     4801 aacttgcgct gcgactactg cttctacctg cgcgcagagg agctgtatcc cgggccggcg
     4861 cgccatgtca tgccggatga tgtgcaggag cagatgatcc tcaatctgct cgagtaccgc
     4921 tttccgcaga cggtatttgc ctggcagggc ggcgagccga cgttgtgcgg gctcgatttc
     4981 ttccggcgga cagtcgagct tcagcagaag tacggcgcgc cgggccagag tgtgggaaac
     5041 gccctgcaga ccaacgggat actgctcgac gcggagtggt gccgtttctt tcgcgaatac
     5101 cagtttctta tcggcctgag cctggacggt ccgcaagatg tgcacgaccg tttccgggtg
     5161 aacatgtcgg ggcggggctc ctgggaccag gtgatgcggg cggccgagct gatgaaccgc
     5221 caccgggtcg agttcaacat cctgtgcgtc gtcaacggcc agaacgttga gatgggcgcc
     5281 gatctgctgc gctggtatat cgatcaaggc ttttcctacc tgcagttcat cccctgcgtc
     5341 gaggcagggt acggccacag cgtgccgccg gagaaattcg gcaaattcct gtgcgatatc
     5401 tttgatttct gggcgaaaga cggatttggg cgggtatcca tccgcgattt tgacgccatg
     5461 ctggcgcagc ggatgggaca gggccagccg atgtgcactt ttggccgcgt ctgcaaccat
     5521 tacgtggtgg tcgagcacaa cggcgatgtc tatccgtgcg attttttcgt ctacgaccac
     5581 tggaaactcg gcaacctgat ggatgcgccg cttcacacct tcctcgaatc ggaaacctac
     5641 cgtaaattcg cgtatcagaa gagcaaggtg ccggcctgcg cgggctgcgc ctggcgcccg
     5701 tggtgccacg gcggctgcca gaaagaccgg ttgctccagg gcggcgattt ccggcagccg
     5761 acggtgctgt gcgaggccta caaaatgttc tttgcacacg cggcgccacg cctgaacgcg
     5821 ctcgcgaaga agatggcccg gtcgcgcggg tgaggaaacc ggcctccaga aatccccgcc
     5881 tcagaattcc agggggcgcg cgttgtattc ttttacaagc aacgcccgcg cctggccggc
     5941 ggtatagacg gacccggtga tgagcagagg gcggtcagcc gttgcggcgc gcattcccgc
     6001 gcagatcgcc tcgtcgatac agtccacccg ttgataggcg gcgtttcccg cggcagcgca
     6061 gagctgttcg acggggagcg cgcggtgccc ggcgaactga gtcaggataa gcggctcggc
     6121 ggtcgttgcc agatagcgca ccatctccgc ggcgtttttg tcagacgaca ccgccaggat
     6181 cgccgcgcat tgcggtacct gccgaacgag ctgccggata ccggccggat tgtgcgcaat
     6241 gtcgatgatg acggggggcg tatccagtac gcgttccagc cggcatggcc agcgggccgt
     6301 gcgaagccct gcgagcatgg cccgggagtc gagctgcggg aaggtctggg cgaggcactc
     6361 ggcgagggct gcggccaggg ctgcattgaa cgtttgaaag gcgcccggaa ggccgagcgt
     6421 aacgggcccc agagtcatcc tctcgctccg gtatgtgaag gccgggttgg cagaatgccc
     6481 ttgcatctca aagcggaaat cgcgctccat caggcggacg ggacagccca gttcgccggc
     6541 gcgcgcgagg atgacatccc gcggtccagg ccgggtttcg ccgatgacgg ccggtacgga
     6601 ttccttgaga atgccggcct tctcgaaggc gatcttctcg agggtatcgc cgaggtactg
     6661 ggtgtgctca agatcgatgt tggttatcgc ggccgccacg ggcgtaatga cgttggtggc
     6721 gtcgaagcgc ccgcccagcc ccacttcgat cagggccaga tcgacttcgt gttgctggaa
     6781 caggtgaaaa gcagccgcag tgaccagctc gaaataggtc ggcggcggcg tcatctgctc
     6841 ggcgatgcgc cgaaaatacg ccagggaggc atccagagct gcgtcgtcaa ccggacgggc
     6901 gtcgatgagg aaccgctcgc gaacatcgat gatgtggggg ctggtgtacc ggcccgtgcg
     6961 cataccggcc gcccggcaca tggcgtcgac catggccagg acgctgcctt ttccgttggt
     7021 gccggcgaca tgcacacagg ggtaccgtgt ctgtgggtta ccggcacagg cgaggagatg
     7081 gcggatgttc tcgaggccca gcttgacgcc gtggaactcc agactggcga ggtattcgcg
     7141 cgcgttcact gcggtttcag gtacagaagg agccgggcca gcatcggccg catttccgtg
     7201 cgcgggacga tggcatcgac gaacccgttg gcgaactggt attcgctggt ctgaaagccc
     7261 tccgggatgt ggactttgag cgcgccttcg ataagccgtt tccccgcgaa accgatttcg
     7321 gcgccgggct cggccaggac gacgtcgccc aggctggcga agctggcgta gacaccgccg
     7381 gtggtggggt tggtgagcac cgagatgtag ggaatcgcgg cttcgttgag tgcgcgaacc
     7441 gcgtcggcgg ttttcgccat ctgcatgagg cccagggtgc cttcctgcat ccgggcgccg
     7501 cccgaggtcg cgaacgagac aagggggacc cgctcccgga tggcgtcgtc ggcggcacgg
     7561 cagaattttt cacccaccac ggagcccatg ct
//
LOCUS       DAOPBQ010000110         7583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8778_length_7583_cov_3.572662, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000110 DAOPBQ010000000
VERSION     DAOPBQ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8778_length_7583_cov_3.572662"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1940)
                     /locus_tag="PKW60_05130"
     CDS             complement(99..1940)
                     /locus_tag="PKW60_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPJ98846.1"
                     /translation="METSAFMGTRLDLIVVFVYFAGIVAFGSYFARYTRTTKDFFFGG
                     QRFAWWLIAFSCIATTVGSYSFQKYSEQGFTYGISSSQTYLNDWYWMSILLLIWLPII
                     YYQRLGSIPEYFERRFGPVSRGFATVIILLYLIAYIGINVYTIGVVLHSLLGWPVFTG
                     AVVTTVLVTLYVMIGGQTSVIMTDLAQGIILLAAGLAVFIAGVVHLGGFVDFWALLPQ
                     GHRFAFSEFNAPDKFSFIGVFVQDGLSNSGAFILMNQGIVMRFLAIRSVQDARKMSVC
                     WVLVLAPLAAVATSGAGWVGRALVENGELEMGATDAFVHTAWFLCAPGVFGFVLAALT
                     AALMSTADTLINAVSAIFVNDIWKRRVRPDADDAHYLKVARIVSLAAGALGLALVPIY
                     YYQYESIYYAHAVVTGSIPVPMVMAILFGILWKRYTPAAAVATLAGGLFLVGLSFVSP
                     LDKWLIGPFAFGMGSGSYGFMRALYGFIVCGILGVTVSLVTKPKPLSELTGLVTGTQL
                     EAMRLYKGGEVNRNPGKKARVRVQVNASLPDPETVIVPKAAFAVMAARPGDILYVSDP
                     RWWFGGLRSVHVRAGEPGDGEALQIHPDALADAHLRDGQAADVEVLL"
     gene            complement(1940..2164)
                     /locus_tag="PKW60_05135"
     CDS             complement(1940..2164)
                     /locus_tag="PKW60_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98847.1"
                     /translation="MDMARVWPIVCQYGIGGILLALGIWCGISSGYFDLSLRRDRNLL
                     WVLVGGYLFLLALVSLFTFWLPYVPKEAGA"
     gene            2581..3408
                     /locus_tag="PKW60_05140"
     CDS             2581..3408
                     /locus_tag="PKW60_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ98848.1"
                     /translation="MKFTRRQFLGFASAFGLASCAKAKRAVPDLNLTRFVPSFGGDEF
                     TFATLNDLHVLDAKSVGIVNQAVNSINADRRVQFTIVLGDMATDGRYEQLKLAKVALD
                     RLEKPCFAVPGNHDVDMKAKDIFSSYTSTYGPAHWDQGKEGWMFIGLNSCVGSESDVT
                     IPQEELDWLEKTLKKVNRSRPIALFAHHPFNPNTKAYRVLNADDVLGLFAEHRLKLVA
                     AGHFHGNQVEEQDGVLFTTTACCSSTRNNFDKTTEKGYRLYHVTKESLETEFVVVRS"
     gene            complement(3448..3798)
                     /locus_tag="PKW60_05145"
     CDS             complement(3448..3798)
                     /locus_tag="PKW60_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPJ98849.1"
                     /translation="MYVPEPEEDDLFDEYDDADAAEDTSGMAVLYVCAGGGCQYKNSQ
                     KIRARLKELVKERGLEDRIRVKKSECMGMCGRGPIVKLTLGNLIFMRVKPKKAEAILD
                     AAEQALCARLVSGE"
     gene            complement(3963..>7583)
                     /locus_tag="PKW60_05150"
     CDS             complement(3963..>7583)
                     /locus_tag="PKW60_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPJ98850.1"
                     /translation="MDFLEALNDRILVLDGAMGTEIQSRGLTDADFGGGPYRMLSDLI
                     TFSRPEVLRDIHRGYYAAGAHAVETNTFGASPFRLSEYDFSSLDLSSFAPLPMEGDIR
                     TLPVEDLAYHLNLRAAQLACEARAGYAGEPGYDGRPLFVVGSIGPSNRMVSSTEANLK
                     RATFDEISANFEVQVRGLIDGGADVVLFETQQDILETKAAVTGALRAMSERGKRLPIM
                     CQVTVDAFSKMQIFNTDIHAALVTMAGVGIDTFGINCSIGPDLMGKTIEKIARYSPLP
                     VSVLPNAGLPISENGRTVFKFPPDEMAGYLLRYVQEFGVNIIGGCCGTTRAHTEAMAA
                     AVAGLKPTRREPERTVFVSGPQQAIALDSSSGLVMIGERLNVRGSRAVREAVEHGGPI
                     EHHALEEVIREQTVDLGVQIIDVCMDSNVVNTTEVLKEVVHTHTTDFPGAMSLDSFAI
                     DALLEAVKVYPGRPIINSISMESVGDGRTRADVVLEATAAHHPVYIALATGPQGPGAT
                     AEQKVALAKQVVDCAARYGVTPDQLMIDMNVFPIGSEAQEGTNFALESLNAIAGIKAI
                     DSRLLTTCGVGNLTNGLAAKPYMRKVLTSVWLDEARKRGLDAAIINPNHYVFVQDLDP
                     EDYALGLRVVLEHDMDAFERLEDIADSKKGIAVSRRSSYEGMALEDAICAKIKDGHKD
                     RTEGAVTVGGVEYPYQDRIVIQAAEVIQHCDPLEFINTHLMKAMNELGDEFARGSVSL
                     PHLLKSADVMKQVMGFLEAYMKRSAGIDVHEQVAYKGTIVLGTVHQDVHSIGKDLAKT
                     LFENYGYRVVDLGVMTPLQAYIDAAREHGATAIGMSALLVQTSNHMITVSRMLAEQGM
                     EALPLLLGGAPVNWRHAAYVAMAGQDDPSKIRSNVFYCPTAMDGVNVMNRWAGTQERD
                     AILMPNRDKLLANLERAEKKRDEEARLMQELAPRVVHFENHQVPDSPWFRSDTLRYSL
                     RQLAPYLDTRTLFGLNWKFGGTQSREKRGETAEKLMALFEEWIDKADKGRWIQPQGVF
                     GVYPCQSVGDEVIVYDPDDPGVELCRFGFTRVLGAMHKDTVCAAQYFYPRASGKMDAI
                     GVQLTTSGPQVEAQIAKFRAEGNSEAVLYLQGLSDRVAEDMADRVHALLRDRMGAAPG
                     QGIRWSPGYPAMAETRYNRVILDLLNATARVGVRITDAGEFAPTGSTAAVVCFHPDAR
                     YT"
BASE COUNT         1567 a         2431 c         2165 g         1420 t
ORIGIN      
        1 ttgctctcct gttggcggct tcatctgtgg tgagtatagc aggccctgta catcatgcgc
       61 ccggccggga agcgcccgaa ttcaatgagt tttcgggatt agagaagcac ttccacatcc
      121 gcggcctgtc catcgcgaag atgggcatcc gccagcgcgt ccggatggat ctgtaatgcc
      181 tcgccatccc cgggttcgcc cgcgcgaaca tgcacggaac gcaagccgcc gaaccaccag
      241 cggggatcgg agacatagag aatatcgccc ggcctggcgg ccatcacggc gaaggccgcc
      301 ttcggcacaa tcaccgtctc gggatccggc agagaggcat tcacctgcac gcgcacgcgg
      361 gcctttttgc cgggattccg gttgacttcg ccgcctttgt acagccgcat ggcctcaagc
      421 tgggtgccgg taacaaggcc cgtgagttcg ctcagaggct tgggttttgt gaccaggctg
      481 acggttacgc cgaggatgcc gcagacaatg aacccgtaca aggcgcgcat aaaaccgtaa
      541 ctgccgctgc ccatgccgaa ggcaaacggc ccgataagcc atttgtcgag cggggatacg
      601 aacgaaagcc ccacgagaaa aaggccgccg gccagcgtcg ccaccgccgc cgcgggggta
      661 taccgtttcc acaaaatccc gaacagaatg gccatgacca tgggcaccgg gatggagccc
      721 gttacgacgg catgcgcgta atagatgctc tcgtactggt aatagtagat cggaaccagt
      781 gcaagtccca gcgcgccggc ggcgagactc acgatgcggg ccactttcag gtaatgcgca
      841 tcatcggcat ccggccgcac gcgccgtttc cagatgtcgt tgacgaatat ggcgctcacg
      901 gcgttgatca gggtatccgc ggtgctcatc agcgccgcgg tcagggccgc cagcacaaat
      961 ccaaaaacgc ccggagcgca aagaaaccag gccgtatgga caaaggcatc ggtggcgccc
     1021 atctcgagtt cgccgttctc gacgagcgcg cggccaaccc atccagcgcc gctggtagcc
     1081 acggccgcca gaggcgccag caccagcacc cagcagaccg acatcttccg cgcgtcctgc
     1141 acgctccgaa tggccaggaa tcgcatgacg atcccctggt tcatcaagat gaacgcgccg
     1201 ctgttggaca ggccgtcctg gacgaatacg ccaatgaaac tgaacttgtc gggcgcattg
     1261 aattccgaga acgcaaaccg gtgcccctgg ggcagcagtg cccaaaaatc cacaaatccg
     1321 cccaaatgca ccaccccggc gataaatacg gccaggccgg ctgcaagaag gatgatcccc
     1381 tgcgccagat cggtcatgat gacggaagtc tgcccgccaa tcatgacgta gagcgtaacc
     1441 agaacggtcg tcaccactgc gcccgtaaag acgggccagc ccagcagcga atggagcacc
     1501 acgccgatgg tatagacgtt aatgccgata taggcgataa ggtagagcag gatgatgact
     1561 gtcgcaaatc cccgtgacac aggcccaaag cgccgttcga agtactcggg aatcgagccc
     1621 agccgctggt agtagatgat gggcagccag atcagcagca ggatactcat ccagtaccag
     1681 tcattgagat acgtctggct gctgctgatg ccgtaggtga acccctgttc gctgtatttc
     1741 tgaaagctgt aactaccgac cgtggtcgca atacacgaaa acgcgatcaa ccaccacgca
     1801 aagcgctgac ctccaaaaaa gaaatccttg gtcgtccgcg tgtaccgggc gaagtacgac
     1861 ccgaacgcca cgatacctgc aaaatagacg aacaccacaa tgaggtcgag gcgcgtcccc
     1921 ataaatgcgc tggtttccat caggcgccgg cctcctttgg tacgtagggc agccaaaacg
     1981 tgaacaggga caccagagcc agcaggaaga ggtacccgcc cacgagaacc cagagcaggt
     2041 tcctgtcgcg ccggagtgag aggtcaaagt atccgctgga aatcccgcac cagatgccaa
     2101 gcgccagcaa gatgccgcct ataccatact gacagacaat gggccagact cgagccatat
     2161 ccatagtgag actccccctg taccagtgag actcgcgcat gatagacaca taccgccgcc
     2221 ggtgcaagta agcgggccag cctcacgccg cagcgatcca gaatgccccg gccttgccgc
     2281 ccgactcacg tccgccagag tttttcgtaa ttcggaagga gccgatgtac tcaagccggc
     2341 tcgtgcggtg gaaaacaggc cttggcacgc gaaaacgccg cttttaatga agaaaaaccc
     2401 acgagcggct gcctgactct tcgtataccg ggccagaccg ccggactcag ccgggcagcc
     2461 gtcttggttg acttcgcggt gtttctgagg aaacaatatc aaaaaacagg gtggtcaaag
     2521 acgatttcgg cttcggcagc caaaacgggg tgggatgacc cacgccgggg gacactgttc
     2581 atgaaattca cacgcaggca atttctgggt ttcgccagcg cattcgggct ggcctcatgc
     2641 gcgaaggcca aacgcgccgt accggacctg aatctcaccc gcttcgtacc cagtttcgga
     2701 ggggacgagt tcacattcgc cacactcaac gacctccatg tcctcgatgc gaaaagcgtg
     2761 gggatcgtca accaggccgt caactccatc aatgccgacc gccgcgtcca gttcacgatc
     2821 gttctgggcg atatggctac cgatggccgc tacgaacagc tcaaactcgc caaagtggcg
     2881 ctcgaccgcc tcgaaaaacc ctgtttcgct gttccgggaa accacgacgt cgatatgaag
     2941 gccaaagaca tcttcagcag ctacaccagc acctacggtc ccgctcattg ggatcagggc
     3001 aaggaaggct ggatgttcat cggattgaat tcatgcgtgg gctccgaaag cgatgtgacc
     3061 attccacaag aagagcttga ctggcttgag aaaacactga aaaaggtgaa ccggagccgc
     3121 cccatcgccc tcttcgctca ccatccgttc aatcccaata ccaaagccta ccgcgtgctt
     3181 aatgccgacg acgtgctcgg cctgttcgcg gaacaccgcc tgaaactcgt ggccgccggg
     3241 cacttccacg gcaatcaggt cgaggaacag gatggcgtgc tgttcaccac caccgcatgt
     3301 tgctccagca cccgcaataa ttttgacaag accaccgaga agggctaccg cctgtatcac
     3361 gtgaccaaag agagcctcga aaccgagttc gtcgtcgtgc ggagttagcc gcgcgtcaac
     3421 gctgccggtg gccggcgcac tacctcgtca ttcgcccgag accagccggg cgcacagcgc
     3481 ctgttcggcg gcatcgagta tcgcttccgc ctttttgggc ttcacgcgca tgaaaatcag
     3541 gttgcccagc gttaatttca cgatggggcc gcgcccgcac atccccatgc attccgactt
     3601 tttcacgcgg atccgatcct cgagaccccg ttctttcacc aattccttca accgcgcccg
     3661 gattttctga gaatttttgt attgacagcc gccgccggca cacacgtaca agacagccat
     3721 cccgctggtg tcctccgcag catcggcatc gtcatactcg tcgaacagat cgtcttcttc
     3781 aggttcaggt acatacacgg ctgctctcca tattcttgca ggggaaccgg gaccggcacg
     3841 cggccgtctc gtggaaatgg gtgtcgagct gggttcaaca gagccgttcc gccctcaaat
     3901 ggcctgcaaa tcattcggaa acggggctct gagctaatct acatgtcgtt ttccattgga
     3961 aatcaggtat atcgcgcgtc gggatgaaaa cacactacgg cggccgtact ccccgtgggc
     4021 gcaaactcgc ccgcatcggt gatgcgcacg cccactcggg ccgtcgcgtt cagcaaatcc
     4081 agtatgaccc tattataccg cgtttcggcc atcgcggggt aaccgggcga ccagcggatg
     4141 ccctggccgg gcgccgcgcc catccgatcg cgcaacaggg catgcacccg atcggccata
     4201 tcttcggcga cgcggtccga aagtccctgc agatacagca cggcttcgga gttgccttca
     4261 gcccggaatt tggcgatttg ggcctccacc tgcgggccgc tggtcgtgag ctgaacgccg
     4321 atcgcatcca tcttccctga tgcccgcgga taaaaatact gagccgcaca gacggtgtct
     4381 ttgtgcattg ctccgagcac gcgggtgaac ccgaaccggc acagctccac cccgggatcg
     4441 tccgggtcat atacgatcac ctcgtcgccc accgattggc aggggtagac tccgaacacg
     4501 ccctggggct gaatccaccg gcccttgtcg gccttgtcga tccattcttc aaacagggcc
     4561 atcagttttt cggccgtctc gccccgtttt tcccggctct gcgtgccgcc aaatttccag
     4621 ttcaggccga acagagtcct ggtatccaaa tagggggcga gttgccgcaa cgaatagcgc
     4681 agcgtgtcgc ttcgaaacca gggcgaatcg ggcacctgat ggttctcgaa atggaccacg
     4741 cgcggggcga gctcctgcat gagccgcgcc tcttcgtcac gcttcttttc tgcgcgttcc
     4801 aggttcgcca gcagtttgtc gcggttcggc atcaggatgg catcccgttc ctgcgtgccc
     4861 gcccagcggt tcatgacatt caccccatcc atcgccgtgg ggcagtaaaa cacgttggag
     4921 cgaatcttcg acggatcgtc ctggccggcc atggccacat aggccgcatg ccgccagttc
     4981 acgggtgcgc cgcccagcag gagggggagc gcctccatgc cctgctcagc cagcatgcgc
     5041 gagaccgtga tcatgtggtt cgacgtctgc accagcagcg cggacatgcc gatcgctgtg
     5101 gcgccgtgtt cgcgggcggc gtcgatgtag gcctgcagcg gcgtcatcac gcccagatca
     5161 acgacccggt acccgtaatt ctcgaacagc gttttcgcga ggtcttttcc gatcgaatgc
     5221 acatcctggt gaacggttcc gagcacgatt gtgcctttgt aggcgacctg ttcatgcaca
     5281 tcgatgcctg ccgaacgctt catgtaggcc tcaagaaagc ccatcacctg tttcatcacg
     5341 tcggcggatt tgagcaggtg cggcagtgaa accgaaccgc gggcaaattc atcgcccagc
     5401 tcattcatgg ctttcatgag atgcgtattg atgaactcca gcggatcgca gtgctggatt
     5461 acctccgccg cctgaatgac aatccggtcc tggtagggat actccacccc gccgacggtc
     5521 acggcgcctt ccgtccggtc cttgtgcccg tctttgatct tcgcgcaaat ggcatcctcg
     5581 agcgccatgc cttcgtacga cgagcgccgg gaaaccgcga tccccttctt gctgtccgcg
     5641 atatcttcca gacgctcaaa ggcatccatg tcatgctcga gcaccacccg aagtcccagc
     5701 gcgtagtctt ccggatccag gtcctgcacg aacacatagt ggttgggatt gatgatggcc
     5761 gcgtcgagtc cgcgttttcg ggcctcatcc agccagacgg aggtcagcac cttgcgcatg
     5821 tacggtttgg cggccaggcc gttcgtcagg ttgcccaccc cgcaggttgt cagcaggcgg
     5881 ctgtcgatgg ccttgatgcc tgcaatcgca ttgagcgatt ccagcgcgaa attggtgcct
     5941 tcctgcgctt ccgacccgat gggaaagaca ttcatgtcga tcatgagctg atccggggta
     6001 accccgtatc gcgccgcgca gtccaccacc tgttttgcca gtgcgacctt ctgctccgcc
     6061 gtggcgccgg gcccttgcgg ccccgtggcg agggcgatgt atacgggatg gtgcgcggcg
     6121 gtggcctcca gaaccacgtc cgcccgggtc cggccgtctc cgacgctttc catggagatc
     6181 gaattgatga tgggccgccc cggatacacc ttgaccgcct ccagcagcgc gtcgatggca
     6241 aatgaatcca ggctcatcgc gccgggaaaa tcggtcgtgt gcgtatggac tacttcctta
     6301 agtacctcgg tggtattcac aacgttggag tccatgcaca cgtcgatgat ctggacgccg
     6361 aggtccaccg tttgctcacg aatgacctct tcgagcgcgt gatgctcgat ggggcctccg
     6421 tgctcgaccg cttcccggac tgcccgggag ccgcgcacat tcaggcgttc gccgatcatc
     6481 accagcccgc tggagctgtc cagcgcgatc gcctgctgag gtcccgacac aaacacagtc
     6541 cgctcgggct cgcgccgggt gggtttcaga cccgccaccg cagccgccat cgcctcggtg
     6601 tgcgcccggg tggtgccgca gcatccgccg atgatattga cgccgaattc ctgcacgtac
     6661 cgcagaagat agccggccat ttcgtccggc ggaaacttga acacggtgcg cccgttctcg
     6721 gagatcggca gaccggcatt cgggagaacc gataccggca ggggcgaata ccgcgcgatc
     6781 ttttcgatcg tcttgcccat caaatccggg ccaatgctgc aattgatgcc gaacgtgtcg
     6841 atgccgaccc ccgccatcgt caccagggcg gcgtggatgt ccgtgttgaa gatttgcatc
     6901 ttcgagaacg catccaccgt cacctggcac atgatgggca ggcgtttgcc ccgctcgctc
     6961 atggcgcgca aggcgcccgt caccgccgcc ttcgtttcca gaatgtcctg ctgcgtctcg
     7021 aagagcacca catcggcgcc cccatcgatc aggccccgca cctgcacttc gaaatttgcg
     7081 ctgatctcgt cgaaagtggc ccgtttgagg tttgcctccg tgctcgacac catccggttg
     7141 gaagggccga tcgagcctac gacaaacagg ggccggccgt cgtagccagg ttccccggcg
     7201 tagcccgccc gcgcttcgca cgcaagctgt gcggcgcgca ggttcaggtg ataggccaaa
     7261 tcctcgacgg gcagggttcg gatatccccc tccatcggaa gcggtgcgaa gctgctcaag
     7321 tccaggctgg agaaatcgta ctcgctcaga cggaagggcg acgcgccaaa ggtgttcgtc
     7381 tccaccgcgt gcgccccggc tgcgtagtac ccccggtgga tgtcgcgcag gacctccggg
     7441 cgggagaagg tgatgaggtc ggacagcatg cggtacgggc cgccgccaaa atcggcgtcc
     7501 gtaagtccgc gcgactggat ctcggtgccc atggccccgt ccagaaccag gatgcggtcg
     7561 ttcagtgctt caagaaagtc cat
//
LOCUS       DAOPBQ010000111         7578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8790_length_7578_cov_4.119101, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000111 DAOPBQ010000000
VERSION     DAOPBQ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8790_length_7578_cov_4.119101"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /gene="pyrR"
                     /locus_tag="PKW60_05155"
     CDS             complement(<1..420)
                     /gene="pyrR"
                     /locus_tag="PKW60_05155"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPJ98851.1"
                     /translation="MTVVMDHDAIDAALRRLAQEIVASHKTLSDVALLGILSRGRPLA
                     DRLAACIEAMTGTRLPVGSLATTLYRDDLRSGRVPATIGNETHFDFDVDGMTVILVDD
                     VIAAGRTVRAALDEVMDYGRPQRIQLACLIDRGHRELP"
     gene            complement(699..992)
                     /locus_tag="PKW60_05160"
     CDS             complement(699..992)
                     /locus_tag="PKW60_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPJ98852.1"
                     /translation="MSTKKPMTKAQIVTALADKTGLTKKDVAAVLEEMKEIAYKEAKI
                     GFTIPGLGKLVVVNRKARTGRNPATGESIKIPAKKVLKFRIAKAAKDAIAPKK"
     gene            1394..2056
                     /locus_tag="PKW60_05165"
     CDS             1394..2056
                     /locus_tag="PKW60_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPJ98853.1"
                     /translation="MYLAVFCAIRGNFPALQAILEAVDERGIEMVFNAGNCVGRYPWP
                     NEVIQLLRAHKLPTVQGADDRAVARFLRSSDRLMRKIPENARRGLEWTHAHTASANLE
                     WLGTLPREQRCTLENIDILLCPGAPTGTKERLDGDSAPEVFERQRESARARLILCGGA
                     ETPFARQVQDTLFVCPGAADGVDGARYAIVDTESEPWSAAFHVAPYDAALVARAAGDA
                     GL"
     gene            complement(2125..2823)
                     /locus_tag="PKW60_05170"
     CDS             complement(2125..2823)
                     /locus_tag="PKW60_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPJ98854.1"
                     /translation="MKHLAITALILCLGGMAFAQDAVPAEEAKPAHPDTSTWKNLFKP
                     DLSNADKPEGVWSFENGELTATEDQAIWSKKDYANFIVDLEFKTAEGSNSGVIVYCSN
                     PKDWIPNSVEIQIADDFAEKWADSPGTWQCGAIFGHVAPAKSTVKKPGEWNRMTVTCV
                     GPRIDVVLNGEHVTSMDMTQWTSAKTNPDGSEIPSWLSRPKAELPTKGRIGLQGKHAG
                     APIWFRNIKVKAVK"
     gene            complement(2965..3594)
                     /locus_tag="PKW60_05175"
     CDS             complement(2965..3594)
                     /locus_tag="PKW60_05175"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HPJ98855.1"
                     /translation="MDAGILLTGILVFVARIIDVAMGILRTMMAIQGRMLLAFLLGFV
                     EVIIWVLVVSTVVSQIRQSPALILFYALGFACGNVVGILVEGKLALGNIALNVISTQG
                     GNALAEALRAAGQPVTVFAGMGMSGPVAQLFMVCRRRDIKRMLAIIQEVDAGALIITD
                     MVRGINKSPHRFHQRGVTPFLTTEAPQAKDAPAAPLPQSDGECGREQSA"
     gene            complement(3694..4113)
                     /locus_tag="PKW60_05180"
     CDS             complement(3694..4113)
                     /locus_tag="PKW60_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HPJ98856.1"
                     /translation="MSKKQSVISKFDQARLQEVIDTMKAQSGKREWTRGDELQRKLTE
                     AAAMEPAEIPDDVITMNSIARVRDKESKQEGVYNLVFPSDSKPLEGRVSVLSSLGLAL
                     FGSRIGDVVEWETPKGLRRLEVIGLIYQPESAQHWTL"
     gene            complement(4290..5351)
                     /locus_tag="PKW60_05185"
     CDS             complement(4290..5351)
                     /locus_tag="PKW60_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPJ98857.1"
                     /translation="MTGNRSILGARKRRQPVDSAILDAAELVFGRKGYQQTTMEMLAR
                     EAGISVGTLYNLIEGKEDLYGRVAQRIGEFVMRRLEPLLRASDPEEAVLDLIRLRLCN
                     YANDRLFFQPFCFPAYLGVQPEPERLGPEVNHLHEKYIDLVERIFARCFAKLGQKGTP
                     GIQMSVCLEGMLTAFTSYWSEPLQSDNLAKVARHMRTVLLRGIIPPESPEDGAMAESR
                     AIYISRYDLERLRELLEVVRAFGTHENQKYADMLDEELKQARIANPREVPPDVVTMNS
                     KVRVKNPNTGADRVHTLVFPRNADLAPENVSILNPFGMALLGRRLSDVFTVGPGPDAP
                     MYEIAQILYQPEASGDYHL"
     gene            5962..6381
                     /locus_tag="PKW60_05190"
     CDS             5962..6381
                     /locus_tag="PKW60_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98858.1"
                     /translation="MENSRDVSLVQIGPVVVATFETASLKNGVWQEAFQQMQGLLKDS
                     RPTHLLLDWFRVDDLAADTLTDLAKLVNAADAVGGSLRLCGLPEKLRGQFEAARLDGL
                     VLGEDVPDALPRYVCLLHGPAETGVDGSVSRILQNSI"
     gene            6460..7080
                     /locus_tag="PKW60_05195"
     CDS             6460..7080
                     /locus_tag="PKW60_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HPJ98859.1"
                     /translation="MARKTKEESEETRKAILLAALDVLSEKGYSRTTFVDIARRIGMS
                     KGAVYWHFKNKQALLAALMEEMRGREEALLAGEAGELTSLDGLKAYLVARMRIATRDD
                     LCRKFAFFMLLQMEWSVDVIEAVRRELMNLRKEPFDEFTSILNEARCRGELRPDIDIA
                     TAKDMLKGMWIGPLTVYFQGLSATDPVQTAEIALGALIDSLRAQHR"
     gene            7092..>7578
                     /locus_tag="PKW60_05200"
     CDS             7092..>7578
                     /locus_tag="PKW60_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPJ98860.1"
                     /translation="MTNDSHEQGGAAQPRRPNRLLGVAQAVFVLVLLGAGWFLRGLMP
                     SGPAGGPAGMPAGAMGGPQGPPAVVVAAVTEGPAEAPEEFIGRVESIESVDVTPQIAG
                     YLQTVHFEEGSRIAAGDLLFTIEKAPFEARVALAEAAVHQAEANVPAAQADLDAARAN
                     LV"
BASE COUNT         1366 a         2316 c         2332 g         1564 t
ORIGIN      
        1 gggcagttcg cggtggccgc ggtcgatcag gcaggccagc tggatgcgct ggggccgccc
       61 gtaatccatc acttcgtcga gcgccgcccg gacggtccgc cccgccgcga tcacgtcatc
      121 caccaggatg accgtcatgc cgtccacatc gaaatcaaaa tgggtttcgt ttccaatcgt
      181 cgcgggcacg cggccgctgc gcaggtcgtc gcggtagagg gtcgtggcca gcgagccgac
      241 cggcagccgt gtgccggtca tggcttcaat acaggcggcg aggcgatccg ccaggggccg
      301 gccgcggctg agaatgccga gcagggccac gtcggacagg gtcttgtggc tggcgacgat
      361 ctcttgcgcc aggcggcgga gtgccgcgtc aatggcgtcg tggtccatca cgaccgtcat
      421 gtccggggag gagtcagctt cgctcatgag ccatcctgcg ttttcgtttc gcccgggtac
      481 gccgaaccgg gccgataaaa aaaaggcctt ctcatgtggt gagaaggtgc tgtgcgctcg
      541 tcacggtttt cactgggata cgcccctccg ggtcggttgg acggatctta gcaaccggcg
      601 cgaacgctgt caactcatgc gtccgcagct gctgcgtaca cgaaaaccgg tccggaacgg
      661 ttccgggccg gttttcgtga attcgggcgg gtctccgctt atttcttcgg ggcaatcgcg
      721 tctttcgcgg ccttggcgat gcggaacttc aagacctttt tcgcggggat cttgatggat
      781 tcgccggtag ccggattgcg cccggtgcgg gctttgcgat tgacgaccac cagtttgccg
      841 agtcccggaa tcgtgaagcc gatcttggcc tctttgtacg caatctcttt catttcttcg
      901 aggacggccg cgacgtcctt tttggtcagg ccggttttgt cggccagcgc ggtcacgatc
      961 tgcgctttcg tcatgggctt tttcgttgac atttggattc tcctctcggc atcgcgaaag
     1021 ccgctcaggc tttccgaaaa caccgccgcg gaattggaag gcgcaacagg cgcctactcc
     1081 tcacggcaat ttgactcccc aaagggcctc ttgcttcgtt ggcccgcacg tttgattaca
     1141 aataaaataa cgttttgtca agccttattt ttcaggggca ttcagatttt cggctcccaa
     1201 aacccttgcc agcatggacg aatcgcgatc cagcccactt tgacgtcttt gtggacattc
     1261 tgtaacacat tgctttattt gcggttacaa aaccttgcga gacgtccgat cgggccgcta
     1321 tagtatgccg cagggtttga aaagagcgcc gggggagcgt ttcgcaacag acagggagtg
     1381 tggttttttc gtcatgtatc ttgccgtttt ctgcgcgata cggggcaact ttcccgcatt
     1441 acaagccatt ctggaggccg tagacgaacg cggcatcgag atggtgttca acgccggcaa
     1501 ctgcgtcggc cggtatccct ggccgaacga agtcatccaa ttgcttcgcg cacacaagct
     1561 ccccaccgtg cagggggccg atgatcgtgc ggtcgcacgt tttctgcgca gcagtgaccg
     1621 gctgatgcgc aaaattccgg agaacgcgcg ccgcggcctg gaatggactc atgcccacac
     1681 ggccagcgcc aatctcgaat ggctgggcac gctcccccgc gaacagcgct gcactctgga
     1741 gaacatcgac atcctcctgt gccccggcgc tcccaccggc acgaaggaac ggctggatgg
     1801 cgattcagcc cccgaggtct tcgaacgcca gcgtgaatcg gcccgggccc gactcatcct
     1861 ctgcggcgga gctgaaacgc cattcgcgcg gcaggttcag gacaccctct tcgtctgtcc
     1921 cggtgctgcg gatggagtag atggcgcgcg gtatgcaatc gtggacacgg agtccgagcc
     1981 ctggagcgcc gcgttccacg tggcgccgta cgatgccgcc cttgtggcgc gcgcggcagg
     2041 cgacgcaggg ctgtaagcaa gcttcgcggg gcgggcccgg gcgtccaggc cgaagcgcca
     2101 tgggcagcgt cggccgcgcg tgtcctactt caccgccttt accttgatgt tgcggaacca
     2161 gatgggcgca ccggcatgtt tgccctgcag tccgatgcgg cctttggtgg gcagctcggc
     2221 cttggggcgg ctcagccacg acggaatctc cgaaccgtcg ggattggtct ttgccgacgt
     2281 ccactgggtc atgtccatcg acgtgacatg ttctccgttg agcaccacat caatgcgcgg
     2341 acccacgcag gtcacggtca ttcggttcca ttcgcccggc ttcttcaccg tgctttttgc
     2401 gggcgcgacg tggccgaata tcgcgccgca ctgccaggtc ccgggcgaat cggcccattt
     2461 ctcggcaaaa tcgtcggcaa tctggatctc gacggaattg gggatccagt ctttcggatt
     2521 gctgcagtag acaatgactc cgctgttgga gccttcggct gtcttgaact ccagatcgac
     2581 gatgaaattg gcgtagtctt ttttcgacca gatcgcctgg tcttcggtcg cggtcagttc
     2641 gccgttttcg aaagaccata cgccttccgg cttgtccgca ttggagagat cgggtttgaa
     2701 cagattcttc caggtactcg tgtcggggtg ggccggtttt gcctcttccg ccggcacggc
     2761 gtcctgggcg aacgccatcc cgcccaggca cagaatcaat gcggtaatcg ccaggtgctt
     2821 catcatgtct ccattctccc ggacagtcag ccccgctccg gtgcgccgta ccccggcacc
     2881 accggataca gggcgtttat gcgcgccatg ttgacggcgt ccaagccgcc ggatgcctga
     2941 ttgtaaaacc gggtccctcc actatcaagc gctctgttcg cgcccgcatt ctccgtcgga
     3001 ttgaggcagg ggcgccgctg gggcatcttt cgcctgcgga gcttctgtgg tgagaaaagg
     3061 tgtgacaccg cgctgatgga accggtgcgg gcttttgtta atgccccgga ccatgtcggt
     3121 aatgatcaag gcgccggcat cgacctcctg aataattgca agcatccgtt tgatgtcccg
     3181 gcgccggcac accatgaaca attgcgcgac agggccggac atccccatgc ctgcgaagac
     3241 ggtcacgggc tgccctgccg cccgcagcgc ctcggccagc gcgttgccgc cctgggtgga
     3301 gatgacattg agcgcgatgt tccccagcgc gagctttcct tcaaccagga tccccaccac
     3361 gttgccgcac gcaaaaccaa gcgcataaaa caaaatcagc gccggcgact gccgaatctg
     3421 actcacgacc gtgctgacca cgaggaccca gatgatcact tccacaaaac ccagcaaaaa
     3481 cgcgagcagc atccggccct gaatggccat catggtccgg agaatgccca tggccacatc
     3541 gatgatgcgc gcaacaaaga cgagtatgcc cgtcagcaga attcccgcgt ccatctccat
     3601 ctctcctggc gagccgcccc ggcgcggcgc ggcggacgag gccgcccgcc tccgctggcg
     3661 ggaagaagca tccgggaatc gggcggcggc cggctacagg gtccagtgct gtgcggattc
     3721 cggttggtag atcagcccga tgacctcgag gcgccgaaga cctttcgggg tctcccattc
     3781 gaccacgtcg ccgatacgcg acccaaacag cgccaggccg agagacgaca ggaccgagac
     3841 ccgcccttcc agcggtttgc tgtcggaagg aaacaccagg ttgtacacgc cttcctgctt
     3901 ggattccttg tcgcgaacgc gcgcaatgga gttcatggtg atgacatcgt cggggatctc
     3961 cgccggctcc atggcggccg cttcggtgag ctttcgttgc agttcgtcac cgcgggtcca
     4021 ttcccgttta ccgctctggg ctttcatcgt atcaattact tcctgcagac gagcctggtc
     4081 gaatttgctg atgaccgatt gttttttgct catcgcgtac actcctttcg ctgcgaggtc
     4141 tcgaccggcc gacggccgtt atcataccgc tgcaactgac ccctcacatt cgccggcgcc
     4201 gcgtccatct gccccgtgag cgcccttcgg ggggatatcg tggaatagtc aggaacggtc
     4261 tcgacgcctg tcgcgcattt attcacggct tacaggtgat aatccccgga agcctccggc
     4321 tgatacagaa tctgggcgat ctcatacatc ggggcatccg gccccgggcc tacggtgaac
     4381 acgtcgctca agcgccggcc cagaagcgcc atcccgaacg gattcagaat ggacacgttt
     4441 tcgggggcca ggtcggcgtt tcgcggaaac accagcgtat ggacccggtc cgcgccggtg
     4501 ttggggttct tcacccgcac ctttgaattc atggtcacca catccggcgg cacttcgcgc
     4561 gggttcgcga tgcgcgcctg tttcaactct tcgtcgagca tgtccgcata tttctggttt
     4621 tcatgcgtcc caaacgcgcg caccacttcc aacagctccc tcagacgctc gagatcatag
     4681 cggctgatgt agatggcgcg cgattccgcc atcgcgccgt cctccgggct ttccggagga
     4741 atgatccccc ggagcaggac ggtacgcata tgccgggcca ctttggccag gttatccgac
     4801 tgcaacggct ccgaccaata gctggtgaac gccgtcaaca tgccctccag acagaccgac
     4861 atctggatcc ccggcgtgcc cttctgcccc agtttcgcga agcatcgggc gaagatccgc
     4921 tcaacgaggt cgatgtactt ctcgtgaaga tgattgacct cggggcccag gcgttcaggc
     4981 tccggctgca cgcccaggta ggccgggaag cagaacggct gaaaaaacag ccggtcgttc
     5041 gcatagttgc acaaacgcag ccgaatgaga tcgagcaccg cctcttccgg atcgctcgcc
     5101 cgcagcaacg gttccagtcg ccgcatgacg aactcgccga tacgctgggc cacccggcca
     5161 tacagatctt ccttgccttc aatgaggtta tagagggtcc ccacggagat gcccgcctca
     5221 cgcgccagca tttccatggt cgtctgctgg taacctttgc gtccgaacac cagctcagcc
     5281 gcatcaagaa tggcgctgtc tacaggctga cgccgtttcc tggctcccag gatagaacgg
     5341 ttaccggtca tcgctgccgt ccttctccgc cggatgaatt gccattctaa tctgaatacc
     5401 agttcattct actgccggca tccctatcag tcaaccccgc tcaagccgga tcgccgcata
     5461 agcacgtcat gacaggccag atcgccggga agcgcgttgc gttcaggaaa gcgttcgcag
     5521 taccgtgggc gcagcaggta cctgcgcggc gcttgacata ccttctcgca ggtatgtata
     5581 atggctgcga tgctgaatga ggccgcgttg gattagatgt ggtgggtttt cgtggacgtg
     5641 tatgcgggag atacgggaaa tgcaggagga gagcggtgtt gcggagggtt ttttcggggc
     5701 gcagtccgcg caccggaaca gaaacgcatg tcgctcctgc cccgcgccag gtggagccgg
     5761 tttgggcttt ctttctggaa tcttccgcgc ttcatccgcg ttgcgttctg cggcctgtta
     5821 tgttgatagc acttatcttg cccctagacc cccgtccccg ccgggcgtgt ccccctcatg
     5881 caggcccggc ggggaccgcc ccctttccca gggcgggcgc gtggagtgcc gcggggctgc
     5941 actaccagaa ggaatccaga aatggagaat tccagagacg tgagtctggt tcagataggt
     6001 ccggtggttg tagcgacatt tgaaacggct tctcttaaga atggggtctg gcaggaggcc
     6061 ttccagcaga tgcaggggct tttgaaggac tccaggccaa cccatctcct gttggattgg
     6121 ttccgggtgg acgatctggc ggcagacacg ctgaccgacc tggcgaagct tgtgaacgcc
     6181 gctgatgcgg ttggcggctc gctacgctta tgcggcctgc cggaaaagct acgcggccag
     6241 ttcgaggcgg cccggctcga cggcctggtt ctgggcgagg atgttccgga tgcacttccc
     6301 cgctacgtat gtttgctcca cggcccggcg gaaaccgggg tggacggcag cgtcagcaga
     6361 attctccaaa acagcatttg acttacattc ctggaggtat gtatactgac gcgtctctcc
     6421 gcgaacacgc cgtgactttc cttcatgcag tgagttctta tggcacgaaa aacaaaagaa
     6481 gagtcggaag aaacgcgcaa ggccattctg ctggctgccc tggacgtctt gagcgaaaag
     6541 ggctattcgc ggacgacgtt tgtggacatc gcgcggcgca ttggcatgag caaaggcgcg
     6601 gtgtactggc atttcaagaa caagcaagcg ctgcttgcgg cgttgatgga ggaaatgcgc
     6661 ggccgtgagg aggcgttgct cgccggggaa gctggagagc tcacgtcgct ggacggtctg
     6721 aaggcgtatc tggtagcgcg gatgcgcatt gcgacccgcg acgatctgtg ccgcaaattc
     6781 gcttttttca tgctgcttca gatggaatgg tccgtggatg tgattgaggc cgtgcggcgc
     6841 gaactgatga atctccgcaa ggagcctttt gacgagttca cgtccatttt gaacgaggcc
     6901 cgctgccggg gcgagcttcg cccggatatc gacatcgcca cggcaaaaga catgctgaag
     6961 ggcatgtgga tcggcccctt gacggtttac tttcagggtc tttccgcgac ggacccggtc
     7021 cagaccgcgg agatcgccct tggagccttg attgacagtc tcagagcaca gcacaggtaa
     7081 ggagacttcc cttgacgaat gacagccatg aacagggggg tgcggcgcag ccgcgccgtc
     7141 ccaatcgtct actcggagtg gcccaggcgg tgtttgtcct cgtgctgctt ggggcgggct
     7201 ggtttttgcg gggcctgatg ccgtccggtc cggcgggcgg accggcgggg atgccggcgg
     7261 gcgccatggg cgggccccag gggccgcccg cggtagttgt ggccgcggtc accgaagggc
     7321 cggccgaggc gcccgaggag ttcattgggc gtgtcgagtc gattgaatcg gtagacgtga
     7381 cgccccagat cgcgggatac ctgcagacgg tccattttga agaaggcagc cggattgccg
     7441 cgggcgattt gctgtttacg atcgagaagg cccccttcga ggcgcgggtg gcgcttgcgg
     7501 aggcggccgt gcatcaggcg gaagccaacg ttccggccgc gcaggccgac ctggacgccg
     7561 ccagggcgaa tctggttc
//
LOCUS       DAOPBQ010000112         7541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8837_length_7541_cov_3.984237, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000112 DAOPBQ010000000
VERSION     DAOPBQ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8837_length_7541_cov_3.984237"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKW60_05205"
     CDS             complement(<1..401)
                     /locus_tag="PKW60_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98861.1"
                     /translation="MVGIREWHNVTTLHPVGLTVVIVMGVAVLLLPRRFAVYPMIITA
                     SFISSAQRLVIMGLDLDFLRILVLLAWTRMLLRRELEGFAWNRLDTLMVAWMVSGTLI
                     NTLAEGASSALINRCGWMFVGLGMYFYFRCM"
     gene            complement(621..1754)
                     /locus_tag="PKW60_05210"
     CDS             complement(621..1754)
                     /locus_tag="PKW60_05210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPJ98862.1"
                     /translation="MNIGERASAQEVPADVLNRWRKLLSEEERPTPNAEPDRFLATVS
                     ALGSRPVIMEIEDGHGRPGMIIARREPMRVSTRIGYFRVPTPKLDSLVVVYGGILGRI
                     SSESVIDFLTKRLLGTERCDHIMINSLVADSPVMLPLRRLKNAVVHPPILHHRLRLGD
                     TFESIMSRHSGKHRRRLSWERRNLESVFDGKLEWVTLTSEEEVDRGLRDAARIGRRTY
                     HAKVGWLVEENDLWRAQLTLAAQAGELRAHFLVGSAQPIAFVLGWNVHNSYHVVAMAY
                     LPEYARYSPGKHLLLHAIERACVDGMEWVDYGFGDAEYKRVYGTDSWQESTVHIYGRS
                     LRAGAAHVLDITAIRVEQWLRTIAGSSAAARIKKKWRQHVGGP"
     gene            complement(1820..3286)
                     /locus_tag="PKW60_05215"
     CDS             complement(1820..3286)
                     /locus_tag="PKW60_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPJ98863.1"
                     /translation="MSLRRKVVQGSGVIMGGQVLGQGMSFVRNIIVARLISTDDFGIA
                     STFFISISLFEMISNLSVDRLLVQAEDGDDPQFQATAQAFQVLRGAANALLMFLLAWP
                     FSRLFGIPQALWAFQCIALVPLIRGFVHLDPKRLHREMRFGRDVLSELLSQTFALALA
                     WPLAKWFGNYAAVLWLLVGQSLMMVFVSHVTAVRRYAWAWDRAFLLRMAKFGWPLLLN
                     GVLMFLIWQGDRILVGSAYDMTQLALYSVAMTVTATPTDLLSRASSVVLLPPLARAQD
                     YAERFAELYGFLAQALSLGGIIVAVFFIILGPGLVELLYGARYVGVGPFIGWFAVMQM
                     LRLIRTVPTTAALAKGDTVACFTGNVARAACIPVLVILATSTRPLVWLAVAGCLGEVV
                     ALGTILIRMWVRHGLASNIAIIPAIFSVCAAMFAGAATLVTKQGFGLGRLFALCGVLV
                     TSLLGALLFFPHLRFHLFGLLRKPTLLSARTDSKQQGL"
     gene            complement(3283..4566)
                     /locus_tag="PKW60_05220"
     CDS             complement(3283..4566)
                     /locus_tag="PKW60_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HPJ98864.1"
                     /translation="MGAALDTANLGVSALSLATLLGVLARVPSARVTVLDYGRGFRAA
                     AITSEGEEYPFFRYGSANSRRLYRRESIYNLALSSYLGGLGHPLLRLWGESHAVLDVT
                     GGDSFTDLYGNRFFKSITLRKRILLREKASLVLLPQTYGPFASPRNRRIAQDIIRRSA
                     MAWARDERSFEVLRDLLGDAFDPVRHRSGVDVAFVLPSVAPAEDALGDAADWLADRST
                     PLAGLNVSGLLMNQPDAAARQYGFKADYSRAAISLLRRLLRESDARILLIPHVVTPPG
                     HYESDIEACNRVAAAVPEEAGGRVAVLPALRDPREVKWVISQLDLFCGTRMHSTIAAL
                     STGVPAAAIAYSPKTLGVFETCAQGAHVADPRHCDTDEVVERLWRSWSARDDARTSLQ
                     AALPGVLRRAEEQMDVIVRDTLAGPASAAEGGAPP"
     gene            complement(4620..6044)
                     /locus_tag="PKW60_05225"
     CDS             complement(4620..6044)
                     /locus_tag="PKW60_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HPJ98865.1"
                     /translation="MISRWEEEARKQAKIHFTGPQLWGRSPGVYYHEAESAAKAWEID
                     PARLAAKAGTGLDVLAVAEEAAMGSQLANRTVFKSVKRLLPGERIISSASGDAIETCA
                     FPEASSNIIDSLSEQVCEAFRKGAALELSGGLDSSLVLAFGLHRNVKPRISFTIGDEC
                     SADVINATAIAKEFDLPHLRIPVVINREQMREDIVSFIRRSGYSVNAANYAWLPGTLA
                     QLNGMRTAQVSGNGGEICGGFYYTPVDSALEKFSLERIWIASRLLSPRARWSFLWRPD
                     IARDLEAQIYAEVHAALHRVNGTWRERTDAFYRDERIRRWVIPVFDACEALYEVISPF
                     LSPEYIGWATSLDPAVRTRDRRPQRELLWQACPALATFSGVSRQTERAPVFSTIRKTL
                     RRLAGQRQRSALGARESAVALAGDPKIREWIGVMCADSNLEFSTEGVERVLDNPAEYP
                     ELLGVLVNASVAWRDVHQSASEEV"
     gene            complement(6103..>7541)
                     /locus_tag="PKW60_05230"
     CDS             complement(6103..>7541)
                     /locus_tag="PKW60_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98866.1"
                     /translation="PAIGSGPGADAKAIARWDVVPYQTYDGLFEIGVVAFHINGIDRV
                     EFSLDGGPWLAVKEMTLNPRTNVVEYWTVLDATRSPDGPVEIRAIAYPKSGVPRVLQG
                     SLDEKLNVTRGMHSMPLWTNAGGALQELILHVDAVHGDDDTGDGSLSRPFATIARAGN
                     AAGGQVSAFDSVILRLAEGDYILHKGKGGFSHVEPWLTIEPEPGAATSSVRLVGSETG
                     ALSSMKRVRFHRVAIRGVFRIPVLTLWLDGVDYDGMDLSNGDKSIAGAWSPGGIWITG
                     GTATGCRNVLRGADFYRNHHVIECSGCPFGDGPCTLYSRCDTYVLTEFHGDVWHWLVG
                     TTDAASDAADANIIAYNVTAENFATQGIFASVYPNELIGTPIHFNNVALVDINISHRM
                     ENDGDYRGGWWEVGTDHLIMRGVNAPTQVMRWNPEKYNTTVRNTSITDCSFYAFTFTP
                     LPEGTIFYNNHFTNPRLYGAWVPPDYRP"
BASE COUNT         1712 a         2242 c         2084 g         1503 t
ORIGIN      
        1 aacatgcagc ggaaatagaa gtacatccca agtccgacaa acatccaacc gcaccgattg
       61 attagtgcac tggacgctcc ctctgccagc gtgttaatga gtgttccact taccatccag
      121 gctaccatca gcgtatccag gcggttccag gcgaatcctt cgagttcgcg gcgcaacagc
      181 atccgtgtcc aagctaaaag taccaagatc cgaaggaagt caaggtcaag acccataatc
      241 actaatcgct gagccgagga gatgaagctc gccgtgataa tcatgggata tacggcgaag
      301 cggcgaggca agagtaacac ggcaacgccc ataacaatca ccacggtcaa gcctaccgga
      361 tgcaaagttg tcacattgtg ccattctcga atcccgacca ttgcttgtgt gctgtcctat
      421 atgtcgccct ggagtagggt ctcaacactg ctgcggttct tgaagtcagt catccatgcg
      481 tttatgtgcc acagaatctg gagatctctg gagaagcgtc acgagagcct cgatacgatc
      541 caaatcccgc gcgacgaaag aacctgcaat acaacaagtg caaacagacg atttcaatga
      601 agttcatctt gggtgctata ttaagggccg cccacatgtt gcctccattt cttcttgatt
      661 cgagctgccg cagaagatcc ggcaatagtc ctgagccatt gctctacgcg aatagcggtg
      721 atatccagta catgagcagc gcctgcgcgc aaggaacgcc catatatgtg cacggtcgat
      781 tcctgccacg agtccgtacc ataaacacgt ttgtactcgg cgtctccgaa gccgtagtca
      841 acccattcca taccgtccac gcatgcccgt tctatcgcgt gcagcaaaag gtgctttcca
      901 ggagagtacc gtgcatattc cggtagatag gccatggcga ctacgtggta gctgttgtgg
      961 acgttccacc cgaggacgaa agcaatcggt tgggcactcc cgacaagaaa gtgagcccga
     1021 agctcacctg cctgcgctgc cagggtgagt tgggcgcgcc acaagtcgtt ttcttcgaca
     1081 agccagccga ctttggcatg gtaagtgcgc ctaccgattc tagcagcgtc tctcaagcct
     1141 ctatctacct cttcttcgct ggtaagggtt acccactcca gcttgccgtc aaagacagat
     1201 tcaaggtttc ggcgctccca gcttagcctt cgccgatgct taccagaatg ccgcgacatg
     1261 atgctttcga aggtgtcgcc cagtcgaagg cggtggtgta ggatgggggg atgaacgacg
     1321 gcattcttta accgccgcag cgggagcata accgggctgt ccgctaccag actgttgatc
     1381 atgatgtggt cgcatctttc cgttcccaac aatctcttgg tcaggaagtc aattacagac
     1441 tcggatgaaa tcctacccag aattcccccg taaacaacaa ccaacgaatc aagcttgggt
     1501 gtggggaccc ggaaatatcc aatacgcgtg gagactcgca tgggttcccg cctcgcaatg
     1561 atcatcccag gccggccgtg gccgtcctcg atttccataa tcacggggcg ggagccaagc
     1621 gcggacactg tggccaaaaa gcggtcaggc tcagcattcg gtgtcggtcg ctcttcctca
     1681 gataagagct ttctccagcg gttcaggaca tccgctggaa cttcctgagc agaagccctt
     1741 tcgcctatat tcatcttagt tgctcccacg gaaaatcgcc cgttttgtca cgtttgggaa
     1801 tgcttctcca gttctgtgtt tacagacctt gctgctttga gtccgtacga gcactcaaaa
     1861 gtgtcggttt tcgcagcaat ccgaatagat gaaaccgaag gtgcggaaag aacaacaagg
     1921 cgcccaaaag cgaagtgact agcacaccgc acagcgcgaa caacctcccc aagccaaagc
     1981 cttgcttggt caccagagtt gcggcaccag cgaacatcgc tgcgcataca gagaagatgg
     2041 caggtattat agcgatgttg ctcgctagtc catgccgcac ccacatccgt atcaatattg
     2101 taccaagggc taccacctcg ccaaggcatc cggcgacagc cagccacacc aggggtcgtg
     2161 tagaagttgc aaggatgacc aagacaggga tgcaggccgc gcgggccacg ttgcctgtga
     2221 agcaggcaac agtatcgcct tttgcgagcg cggcggtggt ggggactgtg cggatgagac
     2281 gcaacatctg catcaccgca aaccagccga taaaagggcc cactccaacg tagcgcgcgc
     2341 cataaagcaa ttcaactagc cccgggccca gaatgatgaa gaatacggcg acgataattc
     2401 cgcccagact caacgcttgc gcaaggaacc cgtaaagttc ggcaaaacgt tctgcatagt
     2461 cttgcgcccg tgcaagcggc gggagaagca ccaccgagct ggcccggctc agcaaatccg
     2521 tcggagtggc cgtaactgtc atggcaacgg aatacaaggc caactgggtc atgtcgtagg
     2581 cgcttccgac caagatccgg tccccctgcc aaatcaaaaa catgagcacg ccattgagca
     2641 gcagcggcca tccaaatttc gccattctca gaaggaatgc ccggtcccag gcccaggcat
     2701 aacggcggac tgccgtgaca tgcgacacaa agaccatcat gagggactgt ccaaccagca
     2761 gccagagcac ggcagcgtaa ttgccgaacc atttggcgag cggccaggcc agggccaagg
     2821 caaaggtctg agacaggagt tcggagagca catcacgccc gaagcgcatc tcccggtgca
     2881 ggcgcttcgg gtccaggtga acgaagccgc ggatcaacgg tacaagagcg atgcattgga
     2941 aagcccacag cgcctgggga atgccgaaaa ggcgcgagaa gggccaggcc agcagaaaca
     3001 tgagcagagc gttcgcggcg ccgcgcaaga cctgaaacgc ctgcgccgtc gcctgaaatt
     3061 gcggatcgtc gccatcctcc gcctggacca gcaggcggtc gaccgagagg ttgctgatca
     3121 tctcaaacag gctgatggag atgaagaagg tggaggcgat gccgaagtcg tcggtgctga
     3181 tcagccgggc cacgatgata ttgcggacga aggacatgcc ctggcccagc acttggccgc
     3241 ccattattac accgctgccc tgcactactt tgcggcgaag actcacggcg gcgccccgcc
     3301 ttccgccgct gacgccggcc cggcaagggt gtcccggaca atgacgtcca tctgctcctc
     3361 cgcgcgacgc aatacgccgg gcagagcggc ctgcagagat gtacgggcgt cgtcgcgcgc
     3421 ggaccacgat cgccagagcc gctccaccac ctcgtccgtg tcacagtgcc ggggatcggc
     3481 gacatgagcg ccctgggcgc aggtctcgaa gacgccgagc gtcttggggc tgtaggcgat
     3541 ggcggcagcg ggcacgccgg tcgacagcgc ggcgatggtg gaatgcatcc gcgtgccgca
     3601 aaacaggtcc agttgtgaaa tgacccattt gacctcgcgg ggatcacgaa gcgcaggcag
     3661 gacggccacg cggccgcctg cctcctccgg cacggcggcg gcaacgcgat tgcaggcctc
     3721 gatgtcggat tcgtagtggc ccggcggagt cacaacatga ggaatgagga gaatacgcgc
     3781 gtcggattcc ctcaacagcc ggcgaagcag ggatatcgcg gcccggctgt aatcggcctt
     3841 aaaaccgtat tgccgggcgg cggcatcggg ctggttcatc agcagaccgc tcacattcag
     3901 cccggccagc ggcgtgctcc tgtcggcgag ccagtccgct gcatcgccca gggcatcctc
     3961 cgcgggcgct acggagggca ggacgaacgc cacatccacg ccggaacggt gccggaccgg
     4021 atcgaacgcg tcgcccagca agtccctgag cacctcgaag ctgcgttcat cccgcgccca
     4081 cgccatggcc gaccgccgga tgatgtcctg cgcgatacgc cggttgcgcg gcgatgcaaa
     4141 cggaccgtag gtctgcggaa gaaggacaag cgaagctttt tctctcaaga gaatccgttt
     4201 tcgcagagta attgatttaa aaaatctgtt gccgtacaag tccgtgaaac tatccccacc
     4261 ggtgacatcc agcacggcat gactctctcc ccacagcctg aggagcgggt ggcccagtcc
     4321 cccaagatac gaactcaatg ccaggttgta gatgctctca cgtcggtaaa gtcgccggga
     4381 attggcgctg ccataccgaa agaacgggta ctcttctccc tcagacgtga ttgcagccgc
     4441 ccgaaatcct cgcccgtaat ccaatacggt tacccttgca ctcgggactc gggcgaggac
     4501 gcccaaaaga gttgccagcg aaagtgcaga cacgcctaaa tttgccgtgt cgagtgcggc
     4561 gcccatgacg acaaacgaag gtccgtcttt tgtcccagat cgcatatccg cctttactgt
     4621 tatacttctt cggacgctga ctggtgtaca tcacgccacg ctacggatgc atttaccaaa
     4681 accccaagca attccgggta ttccgctgga ttgtccagca cccgttcaac cccttccgtg
     4741 gagaattcca gattactatc cgcacacatc acaccgatcc attctctaat ctttggatca
     4801 ccggccaggg caacagcgct ctcccgagca cccaaagcac ttctctgcct ttgcccagca
     4861 agacggcgca gggtctttcg gatggtcgag aatacgggcg cccgttcagt ttggcgcgac
     4921 acaccggaaa acgttgccag tgccgggcaa gcctgccaaa gcaactcccg ctgcggtcgg
     4981 cgatctcgcg ttcgcacagc ggggtccagc gacgttgccc aaccgatgta ttccggcgaa
     5041 agaaaaggag agatcacctc atataatgcc tcgcatgcat caaaaacggg tataacccac
     5101 cgccgaatgc gttcgtctcg gtagaacgca tcagtccgct cacgccaggt tccatttact
     5161 ctgtgaagcg cggcatgcac ttcggcatag atttgggctt ccagatcccg cgcaatgtca
     5221 gggcgccaca gaaagctcca tctggctcgg ggggacagca ggcgcgaggc aatccaaata
     5281 cgctctaacg agaacttttc aagagcagaa tcgactggtg tatagtaaaa gccaccgcat
     5341 atttcccctc cattgcccga cacctgggct gttctcatgc cattcaattg agcgagcgtc
     5401 cccggcagcc aggcatagtt ggcggcgttc acggaataac ccgagcgccg gatgaaggag
     5461 acgatatcct ctctcatttg ctctcggttg ataaccactg gaattcggag atgaggcaga
     5521 tcgaattctt tggcaatggc tgttgcgttt atgacatccg cggagcactc atcaccaatc
     5581 gtaaaggaga tcctgggctt cacatttcta tgaagcccaa acgcgagcac gagcgaactg
     5641 tccaagccgc cggataattc cagagctgca ccttttcgaa acgcttcgca gacttgctct
     5701 gacaggctgt ctattatgtt ggatgaagct tcgggaaatg cgcaggtctc tatcgcatct
     5761 ccgctggctg acgagatgat tcgttcacct gggagcaagc gcttgactga tttgaacacg
     5821 gttcgatttg ctaactgcga gcccatcgca gcttcttctg caaccgccag tacatccagg
     5881 ccagtccccg cctttgctgc aagtctcgcg gggtcaatct cccatgcctt tgctgcactt
     5941 tccgcctcgt gataatagac tccggggctc ctcccccaga gctgcgggcc tgtgaaatgg
     6001 attttagctt gcttcctggc ctcttcttcc caccgcgaaa tcacaacaga tcatctcctt
     6061 acgcccagcc gcgaattgag aggatgctgg cttgtattgc gttcatggtc gatagtctgg
     6121 cggcacccag gcaccgtata gcctcggatt ggtgaaatga ttgttgtaga atatggtccc
     6181 ctctgggagt ggggtgaacg taaatgcgta aaaactgcag tctgttatgg aggtgttccg
     6241 caccgtggtg ttgtattttt ctggattcca tcgcattacc tgcgtcgggg cattaactcc
     6301 gcgcatgatc aagtggtcag tgcccacttc ccaccacccg cctctgtagt ccccgtcgtt
     6361 ttccattctg tgactgatgt ttatgtcaac gagcgcaacg ttgttgaaat gaatgggagt
     6421 gccgatcaac tcgtttggat atacggacgc aaagatcccc tgcgttgcaa aattctccgc
     6481 cgtcacgttg tatgcaatta tattggcatc cgcagcatcg gaggccgcgt ccgtggttcc
     6541 caccagccaa tgccatacat caccatggaa ttcggtaagg acataggtgt cacaacggga
     6601 gtacaaggtg catggcccat ctccgaaggg gcaaccggaa cactcaatga cgtggtgatt
     6661 gcggtagaaa tctgctcccc gcaatacgtt ccggcatcca gtcgcagtcc ctccggtaat
     6721 ccagattccg cctggcgacc atgccccggc gattgacttg tccccgttgc tcaagtccat
     6781 tccgtcataa tcgacgccat ccaaccacag agtgagtacc gggatccgaa acaccccgcg
     6841 tatggccacc cggtggaatc tgacgcgttt catggagctc agagcgccgg tttccgaacc
     6901 aaccagccgg acagaacttg tggccgcgcc aggttcaggt tcaattgtca gccacggttc
     6961 aacatgggaa aagccaccct ttcctttgtg gaggatgtag tctccttcgg ccaagcgaag
     7021 tattaccgag tcaaaagcag agacttgccc accagccgcg ttacctgccc gggcgatcgt
     7081 ggcaaacggg cgtgaaaggc ttccatcccc cgtatcatcg tctccatgga cggcgtccac
     7141 atgcagtata agctcctgca gcgcaccgcc ggcgtttgtc caaagcggca ttgaatgcat
     7201 ccctcgggtg acattcagct tttcatccaa gcttccttgc agcacccttg gcacgccgga
     7261 ttttggatag gcgatggcgc ggatttcgac ggggccgtcg ggggagcgtg tggcatcgag
     7321 gacggtccag tattcgacga cgttggtgcg ggggttgagg gtcatttcct tgacggcgag
     7381 ccaggggccg ccgtcgagcg agaattccac gcggtcgatg ccgttgatgt ggaacgcgac
     7441 cacgccgatc tcaaagaggc cgtcgtaggt ttgataggga acgacgtccc agcgggcgat
     7501 ggccttggcg tcggcgccgg ggccggaccc gatggcgggg g
//
LOCUS       DAOPBQ010000113         7526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8856_length_7526_cov_4.250435, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000113 DAOPBQ010000000
VERSION     DAOPBQ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8856_length_7526_cov_4.250435"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PKW60_05235"
     CDS             complement(<1..643)
                     /locus_tag="PKW60_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98867.1"
                     /translation="MNRYIIALVFLCTCSITALAQAGQPIPYLEALERSGIALPALGQ
                     PINDALILGNGDLNALLFADGDDLVIRITKNDVWDARVDAALNPPLPTLERLKQLGQG
                     EWPDRNWILPEGYEYTGQDAYHAHPYPCPRACGVVRIPNAAAGLVSAALNLRFAEASV
                     ITDDGVTRVLAPAQANVTLVRSEKMPAAMLEAIASADIPAAETGRQDHLSWLLQ"
     gene            961..1536
                     /locus_tag="PKW60_05240"
     CDS             961..1536
                     /locus_tag="PKW60_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPJ98868.1"
                     /translation="MAEMKSKVFTSGEVAAICGVSPDTVARWFDLGQIEGYRLGPGGD
                     RRIPYSSLRKFMISHGVPLERLEEDEYRILVVDDDPYYLDIIPSALARSGDYRVFVAS
                     TGFDAGAMVVEHTPHVLILDIHLSDMDGRTVCHRVKERQDTRNTRVLAISGYLDDEEA
                     RQLTKQGFDDYLSKPFEIATLVERVNKLLES"
     gene            1603..2196
                     /locus_tag="PKW60_05245"
     CDS             1603..2196
                     /locus_tag="PKW60_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019504792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPJ98869.1"
                     /translation="MKHQVPLVTEAFCLAESLHREQVRKSTNIPYLYHLLSVAALVGE
                     YGGDDRQVAAALLHDAAEDQGGHAALEAIEARLGVEVAAYVRACSDSLTMPKQAWRAR
                     KEAYIDRIAGLAEEVKLIVAADKLHNVRSLLRDYRQAGDALWQRFNGGRAGTLWYYQT
                     AAAALEESWRHPILDELREVVDRLAAVAGGPELQPDA"
     gene            complement(2204..3634)
                     /gene="rpoN"
                     /locus_tag="PKW60_05250"
     CDS             complement(2204..3634)
                     /gene="rpoN"
                     /locus_tag="PKW60_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPJ98870.1"
                     /translation="MMRMEHRIDQRQTLRLMLTQRMQQAIQILQLSGLELDQYVQQEL
                     EANPVLEQKPQTSDPPEGEQREPSDSDEVVDDVAFDLDEFATRLGDYRPTGPDLGRNR
                     DLDSRREFFQNSITKGESFTAMLLRQLRMAAPQERDYRIGERIIGDIDDKGYFTGSEE
                     EIAEELGVPIEDIHHMLGVIQQFEPTGVGARDVVECLLLQIDMEYPEEDELKVLVAEH
                     LEKLERRQIPAIAKAMGIKPERVEELKEMLSKLYPWPGLEYSSDPPQYVTPDVIVEKT
                     DEGYEVYLSNESSPELRISKQYRAWMRKENMSKDEKKYLRDKIESAKWLIRNIEQRQN
                     TILRIAKAIVDVQQDFLEHGVEAIKPLTLQEIADVVGVHEATVSRATRGKYMQTPQGL
                     FEMKYFFSPGLKCDTGDAQSSKSVQSLIKKMIDEEDKTRPLSDQKIANQLKEQGINIA
                     RRTVTKYRENMDILPTTMRRIYQDSK"
     gene            3778..4551
                     /locus_tag="PKW60_05255"
     CDS             3778..4551
                     /locus_tag="PKW60_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPJ98871.1"
                     /translation="MIWFSLLGSGSSGNAILVMTPVAKVLIDCGFSMKQLAFRAAQLG
                     ERLENLDAIFVTHEHGDHVKGLGVLARKLRIPVFMTPGTQARLPVSVAEVPHVECFTA
                     GDSICLDGLSINSFSVSHDAGDPVGFCVEYGGAKLGIAADLGHAPNLVRLRLAGSQAL
                     VLESNYCPELLRQGPYPPQLQQRIRGKEGHLSNEEMSALLSSLRHERLRLVVLSHLSE
                     ENNDPALVFEMARRAVRPCGAEVVVSQQHAPTRLFEVLP"
     gene            4548..5795
                     /locus_tag="PKW60_05260"
     CDS             4548..5795
                     /locus_tag="PKW60_05260"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPJ98872.1"
                     /translation="MTPLTFLRQPEVRELLERASTAAATPVAIHYLLGGDEGPCIVSV
                     GQCAVCRHVHDLPGGSMACRGSRVKASLEALRDGIATPFLCHMGFACVAAPVFPGTSQ
                     GFIITFGPYGPAEAPDALLEDARRGLVAFGEEDPDGLDDLLRSIRLVHSDVVPAIVDW
                     TVRDLNALWEAQQKAAFSEEETPEPPPPTRRARASGVPENDLLHAAAIAAGLAGGNQR
                     EVRSTILTVLSESSAGTRAALAARRARALALTGAVIEAAERARFDVRDCWERLPEFIG
                     MVCSARTNAELATAVTGVLRVIRHNVKTAATDASIEALNKALQLRLDGAVTLNEIAAE
                     LGVPPSTITRRLQRKFGMSFSEYAAKQRVNRAKRLLRTTTLGIGEVALRVGINDPGNF
                     SKMFRKCEGCTPQKYRERFGKKP"
     gene            5792..5950
                     /locus_tag="PKW60_05265"
     CDS             5792..5950
                     /locus_tag="PKW60_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98873.1"
                     /translation="MTTLEKLRHWWLEKRCPTEFLCDYCKYDHPSACLRPERPNAVKC
                     PDYRPKGS"
     gene            complement(6019..6261)
                     /locus_tag="PKW60_05270"
     CDS             complement(6019..6261)
                     /locus_tag="PKW60_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98874.1"
                     /translation="MKYSATSVILPELDVPETCALLQELGYDGCDLAESILDIPQFIS
                     DLNAVGYSGFISIEDFRAMEPRERLKRQLDYLKSLE"
     gene            complement(6687..7199)
                     /locus_tag="PKW60_05275"
     CDS             complement(6687..7199)
                     /locus_tag="PKW60_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HPJ98875.1"
                     /translation="MKEQLVEFFTSTFSLPVATIFLATLPILELRGALPVAILWKEPL
                     PLWQAYALAVFGNMLPIPFIIFLLQPITDFVRRWKHGDAFVEWLFARTRRKGKNIEKY
                     EFWGLTIFVAIPLPMTGAWTGAMAGHLFGLKWYKTLCACFCGVCIAGVIMSALAIFAR
                     GMFIRFFGLG"
     gene            complement(7196..>7526)
                     /locus_tag="PKW60_05280"
     CDS             complement(7196..>7526)
                     /locus_tag="PKW60_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPJ98876.1"
                     /translation="VRIARYCLEHGLPPETVLNVNVPDVPFEALRGSAVTRMGRRDYQ
                     DEIIRRTDPRGGEYYWIGGAEPSHVAAPGTDFEAIQEGRVSITPLHRDVTNRDAMAAL
                     AEWDLSL"
BASE COUNT         1443 a         2182 c         2376 g         1525 t
ORIGIN      
        1 cctgcaggag ccagctcagg tgatcctgcc ggccggtctc cgcggcggga atgtcggccg
       61 aggctatcgc ctcgagcatc gcagccggca tcttttccga gcggaccagg gtgacgttgg
      121 cctgcgcggg agccaaaaca cgggtgacgc catcgtccgt gatcaccgac gcctcagcga
      181 aacgaagatt cagggcagcc gacacgaggc cggctgccgc gttcgggatg cggacgaccc
      241 cgcaggccct cggacaggga taggggtgcg cgtggtacgc gtcttggccc gtgtactcat
      301 acccttcggg aaggatccag ttgcggtcgg gccattcgcc ctgaccgagc tgcttgaggc
      361 gttcgagcgt ggggagcggc gggttcaatg cggcatcgac ccgcgcatcc cacacgtcgt
      421 ttttggtgat gcgaatgacg aggtcatcgc cgtccgcaaa cagcagggca ttcaggtcgc
      481 cgttgccgag aatcaaggcg tcgttgatcg gctgcccgag agcaggcaat gcgatgcccg
      541 aacgctccag ggcttcgaga tacgggatgg gctggccggc ctgggccagg gctgtgatgc
      601 tgcacgtgca cagaaatacc agggctatga tgtagcggtt catgatcgtc cctccagatg
      661 ccgcttagaa taccacgctt tggacgggcc cggagaaccg gctacgcgtg tcgcgcatcc
      721 ggcagcgtac atccacccgt atggcctttt tgtgcaccca tgcgtgcgca tccagaacca
      781 ttaaaaaaac agcccgacac gggattcaac acacggaaat tacagcagtt atgccggttc
      841 ccccggcaca gttcgccgga caggcacgag catttcgctt cattgactga aattgccctt
      901 tatgcaataa tgctccgcaa acaccgaaaa gcactaattt ctccaagtga ggggcggtcc
      961 atggcggaga tgaagagcaa ggtcttcacg agcggcgagg tggcggcgat ctgcggcgtt
     1021 tcgccggata ccgtggcacg atggttcgat ctgggccaga ttgaaggcta tcgcctgggc
     1081 cccggcggag accggcgcat cccctacagc agcctgcgca aattcatgat cagccacggc
     1141 gtgccgctcg aacgcctgga ggaagatgaa taccgcatcc tggtcgttga cgacgatccc
     1201 tattatctcg acatcatacc gtcggcactg gcccgctcag gcgactaccg ggtgtttgtg
     1261 gcgtcgacag gattcgatgc gggcgccatg gtcgtcgagc acacccccca cgtgttgata
     1321 ctggacattc atctttcgga catggatgga cgaacggtgt gccaccgggt aaaagagcgc
     1381 caggatacgc ggaacacgcg tgttctggcg atttcggggt atctggacga cgaagaagcg
     1441 cggcaattga cgaagcaggg ctttgacgac tatctatcaa agccgttcga gatcgccaca
     1501 ctggtcgagc gcgtcaacaa gctgcttgaa agctgacaca ggccgtggcg ggcgtcgcgg
     1561 gcaggcgtgc cggcgatttc atcactgcgc gccggagcag gcatgaaaca ccaagttcca
     1621 ctggtcactg aggccttctg ccttgccgag tcgttgcatc gggaacaggt tcggaagagc
     1681 acgaacatcc cgtatctcta tcatctgctt tcggtagcgg cgctggtagg cgagtacggc
     1741 ggggacgaca ggcaagtggc cgccgcgtta ctgcacgatg cggccgaaga ccagggcggg
     1801 cacgcggccc tggaggccat tgaagcgcgc ctgggcgttg aggtggccgc gtatgtgcgg
     1861 gcgtgcagcg acagccttac gatgccaaaa caagcctggc gcgcgcgcaa ggaggcctac
     1921 attgaccgga tcgcgggcct ggcggaagaa gtgaagctca tcgtcgccgc ggacaaactg
     1981 cataatgtcc gctcgcttct tcgcgattac cggcaggctg gagacgcact ctggcagcga
     2041 ttcaacgggg gccgggccgg aaccttgtgg tactaccaga cagcggccgc ggcgctggaa
     2101 gaaagctggc gccatcccat tctggacgag ctgcgcgagg tggtggaccg attggcggcg
     2161 gtggcgggcg gtccggaact gcaaccggac gcgtgatcga aaattacttg gagtcctgat
     2221 aaattcttcg catggtcgtg ggcaggatat ccatgttttc gcggtatttg gtgaccgtgc
     2281 gccgggcgat gttaataccc tgttccttaa gctgattcgc gattttctga tcgctgagcg
     2341 ggcgggtctt gtcctcttcg tcgatcatct tcttgatgag cgactgaaca ctctttgagg
     2401 attgtgcgtc gccggtatcg catttcaggc ccggcgagaa aaaatacttc atttcgaaaa
     2461 ggccctgggg ggtctgcatg tatttcccgc gcgtggcacg gctgaccgtc gcttcgtgca
     2521 cgccgaccac atccgcgatt tcctgcagcg tcagtggttt gatggcttcg acgccgtgct
     2581 cgaggaaatc ctgctgcaca tcgacgatcg ccttcgcgat gcgcagaatg gtgttttgcc
     2641 gttgttcgat gttgcggata agccacttgg cggattcgat cttgtcgcgc agatatttct
     2701 tctcgtcctt gctcatgttc tctttgcgca tccaggcccg gtactgcttg ctgatccgca
     2761 attcggggga cgattcattg gacaggtaga cctcgtaacc ctcatcagtt ttttcgacga
     2821 tgacgtcggg cgtcacatac tggggggggt cggaggaata ttcgagcccg ggccaggggt
     2881 agagtttgga gagcatctct ttgagctctt caacccgttc gggtttgatg cccatggctt
     2941 tggcgatagc ggggatctgg cggcgctcga gtttctcgag atgctcagcg accagcactt
     3001 tcagctcgtc ttcttccgga tactccatgt cgatctgcaa caggaggcat tcgaccacgt
     3061 cccgggcgcc gacaccggtg ggctcgaact gctggatcac accgagcatg tggtggatgt
     3121 cttcgatggg cacgccaagc tcttctgcga tctcttcctc ggaaccggtg aagtaacctt
     3181 tgtcgtcgat gtcgccgata attcgttcgc cgatgcggta gtcgcgttcc tggggggccg
     3241 ccatgcggag ctggcgcagc aacatcgcgg taaacgactc ccctttggtg atggagttct
     3301 ggaaaaattc gcggcggctg tccaggtccc ggttacggcc gaggtcgggg ccggtggggc
     3361 ggtagtcgcc caggcgcgtg gcgaattcgt caagatcgaa cgcgacgtcg tcgacgacct
     3421 cgtcgctgtc cgacggttcc cgctgctccc cttcgggcgg atcggaggtc tgcggttttt
     3481 gctcgagcac gggatttgct tcgagttcct gctggacata ttgatccagc tccagaccgg
     3541 acagctgcag aatctgaatg gcctgctgca tccgttgcgt cagcatcagg cgcagagtct
     3601 gcctctgatc gatccggtgt tccattcgca tcatgagcac acaatctcct cgaagtgcgt
     3661 gatctgtatt atatcgctga aatgaaaaaa ctgtcaattg gagaggcctg cggaagcctt
     3721 gatataatgc gttcaaatcg gttttttcga cctgtgcatc tcttggggaa caagacggtg
     3781 atctggtttt cactattggg aagcggcagc agcggcaacg ccattctggt catgacgccc
     3841 gtcgctaaag tgctcatcga ttgcgggttc agtatgaaac aactcgcgtt tcgagctgca
     3901 cagctggggg aacgcctcga gaatctcgac gctatctttg tgacacacga gcacggtgat
     3961 catgtgaagg ggcttggcgt gttagcacgc aagttgcgga taccggtgtt catgacgccg
     4021 gggactcaag cccggcttcc cgtgagcgtg gcggaggtcc cgcatgtcga gtgtttcact
     4081 gccggcgatt ccatctgcct tgacggtctg tcgatcaaca gcttcagcgt ctcgcacgac
     4141 gcgggggatc cggtggggtt ctgtgtggaa tacggtggag ccaagctggg catagccgca
     4201 gatttagggc atgcgccgaa tctggtgcgg cttcgtctgg ccggctctca ggcactggtt
     4261 ctcgaatcga actactgccc cgaactgctt cgccagggcc cgtatccgcc acagctccaa
     4321 cagcgcatac gagggaaaga ggggcacctt tccaacgaag agatgtcggc gctgttatcc
     4381 agcctccggc acgaacgcct gcgcctggtg gttctgagcc atctcagcga agaaaacaac
     4441 gatcccgcgc tggtgttcga gatggcgcgc cgggccgtac ggccgtgcgg cgcagaggtg
     4501 gttgtgtctc agcagcatgc gcccacgcgc ctgttcgagg tgctgccatg acgccgctga
     4561 ccttcctccg ccagccggag gtgcgtgaat tgcttgaacg ggcctcgacg gctgccgcga
     4621 ctccggttgc catccactac ctgctggggg gagacgaagg gccctgcatt gtaagcgtcg
     4681 gccagtgcgc tgtgtgccgg cacgtacatg acctgccggg cgggagcatg gcgtgccgcg
     4741 gctcacgcgt caaggcgtcg ctggaggcgt tgcgcgatgg catcgcaacg ccatttctgt
     4801 gccatatggg gtttgcctgc gtggccgcgc cggtgtttcc gggtacgtcc cagggattta
     4861 tcatcacatt tggcccctac ggccccgcgg aagcgcccga tgcactgctc gaagacgccc
     4921 ggcggggtct tgttgcattc ggggaggaag atccggacgg cctggatgat ttgctgcgca
     4981 gcattcgact ggtgcacagc gatgttgtcc cggccatcgt ggattggacg gtccgcgacc
     5041 tgaatgcctt atgggaggct cagcaaaaag cagcgttctc ggaagaagaa accccggaac
     5101 caccgccacc aacgcgccgg gcgagggcga gcggggtgcc ggaaaatgac ctgctgcacg
     5161 ctgcagccat tgccgccggt ttggcggggg gtaaccagcg cgaagtgcgc tcgaccattt
     5221 tgacggtatt gtcggaaagc tccgcgggaa cccgggcggc cctggcggcg cggcgtgcgc
     5281 gggcattggc gctgacggga gccgtcattg aggccgccga gcgggcgcgc tttgacgtgc
     5341 gggactgctg ggagcgtctg ccggagttca tcgggatggt ctgctcggcc cggacgaatg
     5401 cggagctggc gactgcggtc acgggcgtgc tgcgcgtcat ccggcacaac gtaaaaacgg
     5461 cggcaacaga cgcatccatt gaggcgctga acaaagccct gcagctgcgc ttggacggcg
     5521 cggtcacatt gaacgagatc gccgcggaat tgggcgtacc gccaagcacc atcacacggc
     5581 gccttcaacg aaaattcggc atgtcgttct ccgaatatgc cgcgaaacag cgtgtgaacc
     5641 gggcaaaacg gctgctccga accacaacac tcggaatagg cgaggtcgcg ctgcgcgttg
     5701 gcattaacga cccaggcaat ttttccaaga tgtttcgcaa atgtgaaggg tgtactccac
     5761 agaaataccg cgaacggttc ggaaaaaagc catgaccacc ctcgaaaagc tgcggcactg
     5821 gtggctcgaa aaacgctgcc ctacggaatt tctttgcgat tactgcaagt atgatcatcc
     5881 gtcagcgtgc ctgcgccccg aacgcccaaa cgccgtaaaa tgcccggatt accgcccgaa
     5941 aggcagctag attcacgccg caccggcttt ttcgcatctg agacgccgga gttcgcgctt
     6001 aatttgcgcg tccagacctc attccagtga tttcaggtag tcgagctggc gtttgagcct
     6061 ttcgcggggt tccatggccc gaaaatcctc gatcgagatg aatccgctat atcccacggc
     6121 gttgaggtcg ctgatgaact ggggaatgtc gagaatgctc tcggcgaggt cgcatccgtc
     6181 gtagccgagt tcctggagca gcgcacatgt ctccggcacg tcgagttcgg ggagtatgac
     6241 gctggtggcg ctgtatttca tcgctgagca tacctctttt cgtgcatcct gacccgggac
     6301 tcttcccgac gcccagtatc cggcgcaagg cgctgcgttt caagcagccg acgcgaggtc
     6361 tgctgtatca tttcgcgggc ctttccccag aaaatttcct gttttgcagg ttcaccgttt
     6421 tgtgtgttcg ggtaaaagag ccgtttggcc gcatccggat tgcaggggcg cccgcgccac
     6481 gtccgccggc tctcccgcgg gtgacacaag acccggaacc aacgaacatg gcgcttggcc
     6541 tggtctttga tccatcctct ctggcgggaa cacacgagcc cgaagtgcag agatcaatgt
     6601 gcggcgcact tgtccctggc gggggtgaat cggcgttagt cgatttgggg gtggactctc
     6661 cgcgcgggtt ttgggcggcc tgcgctctag ccgaggccga agaagcgaat gaacatcccc
     6721 cgtgcgaaaa tggccagcgc tgacatgatc acgccagcaa tacagacacc gcagaagcag
     6781 gcgcacaggg tcttgtacca cttcaatcca aacaggtggc cggccatggc gccggtccac
     6841 gcgccggtca tcggaagcgg gatcgccacg aagatggtca agccccaaaa ctcatacttt
     6901 tcgatgttct tgcctttacg gcgcgtgcgg gcgaacagcc actccacgaa agcgtcgccg
     6961 tgtttccagc gccgcacaaa atccgtgatg ggctgcaaga gaaagataat gaacgggatg
     7021 ggcagcatgt tgccgaatac cgccagggcg tatgcctgcc acaacggcaa gggctccttc
     7081 catagaatcg ctacgggtaa tgcgccgcga agctccagga tgggcagcgt ggccagaaag
     7141 atggtggcta cgggcagcga gaaggtgctc gtgaaaaatt cgaccagttg ttccttcaca
     7201 gcgagagatc ccattcggcc agagcggcca tcgcgtcccg gttggttacg tcccgatgca
     7261 gcggcgtaat gctgacccgg ccttcctgga tggcctcgaa atcggtgccc ggcgctgcga
     7321 cgtggctcgg ctcggcgccg ccaatccagt aatactcccc gccgcgggga tccgtccgcc
     7381 ggatgatctc gtcctggtaa tcgcgccggc ccattcgggt tacggcgcta cctcgcaatg
     7441 cctcaaacgg cacatcgggg acgttcacat tcagcaccgt ctccgggggc agaccgtgct
     7501 ccaggcagta ccgggcaatt cggacg
//
LOCUS       DAOPBQ010000114         7470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_8935_length_7470_cov_3.941470, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000114 DAOPBQ010000000
VERSION     DAOPBQ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8935_length_7470_cov_3.941470"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..1340
                     /gene="sat"
                     /locus_tag="PKW60_05285"
     CDS             183..1340
                     /gene="sat"
                     /locus_tag="PKW60_05285"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HPJ98877.1"
                     /translation="MAITPHGGTLVNRLLTGNALAAARKKAETLPCITVDAYTAFDID
                     GIAKGLFSPLTGFMGEAETRSVLDTMQLRPGIPWTIPILLDASREQADQLDTGAEVAL
                     RDDVGEVVAILHLTEKFSLDRAELCEKVYLTTDESHPGVKYTLSLGDVFLAGALDVMK
                     AREIEFQDYNLTPVETRAAFESRGWKRIVAFQTRNPIHRAHEYLTKCALEICDGLLIH
                     PLMGTTKSDDIPGDVRMKCYTVLIDHYYPKDHVMLSLMPVNMRYAGPKEAIMHSIIRK
                     NYGCTHFIVGRDHAGVGNFYGTYDAQRIFDQFDPAEIGITPLFFEHAFYSKRTQSMAS
                     PKTCPGTNEDHVFLSGTKVREMLKRGEMPPPEFTRPEVARILIDWAQENGE"
     gene            1337..2728
                     /locus_tag="PKW60_05290"
     CDS             1337..2728
                     /locus_tag="PKW60_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPJ98878.1"
                     /translation="MNRRLLVIGLDCATPQFLFGPDAFDLPNLRALMRRGVWGRLESC
                     HPPITVPAWAAMLSGKDPGTLGLYGFRNRRDHSYSPMVTADGAAVREPRVWDILSRHG
                     KKCVVLGVPQTYPVRPLNGWLVAGFLTPSEEAPYTYPRPLKEELNRELGPYIIDVKDF
                     RTDDRAALLERLYALMENRFAYARYLMTSKSWDFFMMVDMAIDRLHHAFWRFCDPSHP
                     AFEAGNPFERVFRDFYQAVDARVGELIELAGSDTAVMVVSDHGAKGMAGGVCVNQWLI
                     REGLLTLERAPEGRARIEDCAIDWARTKAWSSGGYYARVFFNVEGREPMGQIAAADYD
                     AFRDGMIARIESMRGPDGRLLGNRALKPQDLYARVEGVAPDLLVYWGDLRWRSVGSVG
                     YDDIFTFENDIGPDDANHDYHGVFVLDGRSGAGDRELQGLTLASIAPTMLRLMDVPAP
                     ADMPGPPIVEELA"
     gene            2725..3069
                     /locus_tag="PKW60_05295"
     CDS             2725..3069
                     /locus_tag="PKW60_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98879.1"
                     /translation="MNRPYAVRLTGESAGDAAHVLVRRLVELGCPVCLPDQTPPADFN
                     GVIVLLGNAPSTAPVGETVELSDHDTPDFAAEKVLDLLHDKGVISLEPEGYTPEEEEA
                     IRKRLADLGYID"
     gene            3123..4175
                     /locus_tag="PKW60_05300"
     CDS             3123..4175
                     /locus_tag="PKW60_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPJ98880.1"
                     /translation="MRIGINALSLEPGYCGETVYLRSVFTRIRELQPETRAIFVTSER
                     NHDFFEGFDRAPFNGAVPPPQIGLPAAEREFARTLEAAGVDFLFSPLQTAPAKCRVPL
                     VLYVMDLQFIVEPLARPRWRNKAAVKRVMQSCETATAILASSEFIRRQLLELLDVPLD
                     KVIVAPLGVSDVFAEPQACWVQQPFFLHVGATRASKNIESLLSAFEMFEDRTPHSLVM
                     VGLPGDREQADWGPRIMRIHQCPESALAGLYQHCDALVCASLYEGAGVTVLEGMRAGA
                     QIVAGRVGAIPEFAGNAPIYCNAQNASTIAAAMQRALELDPDKRERRAMYMTHAAAEY
                     TWDKCAWKTLQAFRAR"
     gene            4291..5451
                     /locus_tag="PKW60_05305"
     CDS             4291..5451
                     /locus_tag="PKW60_05305"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPJ98881.1"
                     /translation="MTVSHRTRSALWIVLLLAAWSGGSAVSQENSPAAVPLDPATATT
                     EFGEGLLWYDAANLPIDGKGWNDVAACYDRLPARAEGVVRPPVWNLSRDSAGLAIRFQ
                     TDSPAIAARWVLRDSNLAMPHMPATGVSGLDLYVKLADRWMWVANGRPSQVTMQASLV
                     TGIPEGMREYLLYLPLYNGVTSVHIGINPDSVLAAPPERSETTRSPVLFWGTSITQGG
                     CASRPGMAYPSIVGRMMDRPTINLGFSGNGKLEPEMAAFIAELDVAVFVLDCCPNLKP
                     EEIAERTEPVVQVLREAHPDTPIVLMENIIYQSGYFLPGTRRAYEAKNAELKAAYERL
                     TAAGVTNLHYIPCDNLLGDDAEATVDGTHATDLGFQRMAEAVTPILKSLAGE"
     gene            5489..6691
                     /locus_tag="PKW60_05310"
     CDS             5489..6691
                     /locus_tag="PKW60_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98882.1"
                     /translation="MLNELELFRVRIGLLQVFLKERQYDGVLLSRTDNFAMATAGKRN
                     YVSLCSDLGASSLYVTQAGEAYFVGNNIEETRVVAEELRASGCEIRKFMWFEDSAPAL
                     VQREFSGAIVSDDGSLGKNVNGEMAYIRSLLTPNELEKYRHLGKLAAETITTAVEQTE
                     AGMAEADIAAILIGEGAKRRCQVPIALIAADRRATKYRHPLPTVAPLLPERMREIQVR
                     GYVMIILSLLKEGLVVSATRFKRVGDLSERIPSAYDRICGVDALMYEATEPGKTLGQV
                     FADCQQAYNAMDFAPTEWHNHHQGGTTGYAGRTCKASPSEPFLIFDSSWEDKVRDITG
                     IDVAFGQAFAWNPSAPGVKSEDTFLLMPDGTKEIITRTPALPRVDLTAVLGRKTEAVK
                     SGIAEPGA"
     gene            complement(6710..>7470)
                     /locus_tag="PKW60_05315"
     CDS             complement(6710..>7470)
                     /locus_tag="PKW60_05315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPJ98883.1"
                     /translation="DRMLDMGFLPDIRSIIQKLPQARQTLMCSATFPPEIARLSREFL
                     NDPERIEVGLVAQPVDTVRQVVYTVNPERKIALLAKVLREQQVDSAMIFLRTKPRTER
                     VTRALRRNGFKVQEIHGDRSQRQRDEALAGFRSGKYRYLVATDVAARGLDIENVSHVI
                     NFDIPENPDDYIHRIGRTARANSDGDAITFVSPTDHIALEAIERALGKNLPRADWEEA
                     VPVISLYQAPGEATGRGRRGISYRRRRSGLMKSP"
BASE COUNT         1405 a         2475 c         2166 g         1424 t
ORIGIN      
        1 ggcaggacga taccatctcc ggtaagatct tagatggcgc gattcgccgg tttgagtcct
       61 gccgggtctc ttgctacact tttgcctcct gtattcaggc ggagtcacgc gcctccacgg
      121 cgcggttttg tcttacccgg acgtacgcag tcgcgccggt catcaccaaa gggagatcca
      181 gcatggccat cacaccacac ggcggaacac tggttaaccg gttactcacg gggaacgcac
      241 tcgccgcggc ccgaaaaaaa gccgaaaccc tcccttgtat tacggtcgac gcctataccg
      301 ctttcgacat tgacggcatt gcaaagggct tgttcagccc gctcacgggc tttatgggag
      361 aggccgagac tcgcagcgtc ctggacacca tgcagctccg ccccggcatt ccctggacta
      421 tccccatcct gctggatgcg tcacgcgagc aggcagacca gctcgatacc ggcgccgaag
      481 tcgccctgcg cgacgatgtg ggcgaagtgg tggcgatcct ccatctgacc gaaaaattct
      541 cgctcgaccg cgcggaatta tgcgaaaaag tctatctgac aaccgacgaa tcgcatcccg
      601 gcgtgaaata cacgctgtcg cttggagatg tctttctggc gggggcgctc gacgtcatga
      661 aagcccgtga gatcgagttc caggactaca atctcacgcc tgttgaaacc cgggccgcat
      721 tcgagtcccg gggctggaaa cgtatcgtcg ctttccagac tcgaaacccg atccaccgcg
      781 cacacgaata cctgaccaag tgcgcccttg agatctgcga tggtcttctg atccatccgc
      841 ttatgggcac caccaagagc gacgacatcc ccggtgatgt ccgcatgaag tgctacaccg
      901 tgctcatcga ccactattac cccaaggacc acgtcatgct gtcgctcatg cccgtgaaca
      961 tgcgctacgc gggccccaaa gaggcgatca tgcactccat tatccgcaag aactacggct
     1021 gcacccattt catcgtcggc cgcgaccacg ctggcgtggg caatttctat gggacttacg
     1081 acgcgcagcg cattttcgac cagttcgacc ctgcagagat tggcatcacg ccgctgttct
     1141 ttgaacatgc gttctattcg aagcggaccc agtcgatggc ttcgcccaag acctgtcccg
     1201 ggaccaacga ggaccacgtc tttctgagcg gcaccaaagt gcgtgaaatg ctcaagcgcg
     1261 gcgagatgcc tccgccagaa ttcacgcgcc cggaggttgc ccggatcctg atcgactggg
     1321 cccaagagaa cggagaatga accgtcgcct cctggtgatc gggctggact gcgcgacgcc
     1381 ccagttcctt ttcgggccgg atgcgttcga cctgccgaac ctccgcgcgc ttatgcgccg
     1441 gggcgtctgg ggcaggctgg aaagctgcca cccgcccatc accgttccgg cctgggccgc
     1501 catgctctcg gggaaagacc ctggaactct cggcctctac ggattccgga atcgccggga
     1561 tcattcctac agccctatgg ttacggcgga tggcgcggcg gtacgcgagc cgcgcgtctg
     1621 ggacattctc tcccggcacg ggaaaaaatg cgtcgtactg ggcgttccgc agacctatcc
     1681 cgtccgcccg ctcaacgggt ggctggtcgc gggctttctt acgcccagcg aggaggcgcc
     1741 gtacacgtac ccccggccgc tgaaggaaga gctcaaccgc gagctcggtc catacatcat
     1801 tgacgtcaag gattttcgga ccgatgaccg ggccgccctc ctggaacgcc tttacgcgct
     1861 catggagaac cggttcgcct atgcccgcta tctgatgacc tcaaagtcct gggatttctt
     1921 catgatggtc gatatggcca tcgaccgcct ccatcacgcg ttctggcggt tctgcgatcc
     1981 cagccatccc gcgtttgagg ccggcaatcc ttttgagcgc gtgttccgcg acttctacca
     2041 ggcggtcgac gcccgagtgg gcgagcttat tgagctggcc ggcagcgaca cggccgtcat
     2101 ggtcgtctcg gatcatggcg ccaagggcat ggccggcggc gtctgcgtca accagtggct
     2161 catccgcgag ggcttgctga ccctcgagcg cgcccctgaa ggccgcgccc gcatcgagga
     2221 ctgcgcgatc gattgggccc gcacaaaggc ctggagcagc ggcggctatt acgcacgggt
     2281 cttttttaac gtggaaggcc gcgagcccat gggccagatc gccgctgcgg actatgacgc
     2341 cttccgtgac ggcatgattg cgcgcatcga gtccatgcgg ggtcccgacg gccgccttct
     2401 cggaaatcgc gccctgaagc ctcaagacct gtacgcccgc gttgaaggcg ttgcgccgga
     2461 tctcctcgta tactggggag atttgcgctg gcggtccgtc ggctccgtcg gctacgacga
     2521 tatcttcaca ttcgaaaacg acatcggccc cgacgacgcc aaccacgatt accatggcgt
     2581 tttcgtcctc gacggccgct ccggtgcggg agaccgcgaa ctccagggcc ttacgctggc
     2641 ctccatcgct ccaacgatgc ttcgccttat ggatgtgccc gcgcccgccg atatgccggg
     2701 gccgcccatc gtggaggaat tggcatgaac cgtccgtacg ctgtccggct gacaggggaa
     2761 agcgccggcg atgccgccca cgtgctcgtc cgccgcctgg tggagctggg ctgccctgtc
     2821 tgcctgccgg accagacgcc tccggctgat ttcaacggtg tgatcgtcct gctcggcaac
     2881 gcaccgtcca ccgcgccggt cggagaaacc gtcgagctca gcgaccacga taccccggat
     2941 ttcgccgccg agaaggttct cgatctcctc cacgacaagg gcgtcatatc gctggagccc
     3001 gaggggtata cccccgagga agaggaagcg atccggaaac gattggccga cttggggtat
     3061 attgattagc gatatgcaac cgcactgcat tacaagaatc caggaagctc tcagcgcacg
     3121 ccatgcgaat cgggattaac gcactcagcc tggaaccggg ctattgcgga gaaaccgtgt
     3181 atctccgcag cgtatttacg cgaatacgcg aacttcaacc ggaaacccgg gccatttttg
     3241 tcaccagcga gcgaaaccac gacttttttg aagggttcga ccgcgctccc tttaacgggg
     3301 cggtgccgcc gccacagatc gggttgcccg ctgccgaacg cgaattcgca cgaacactcg
     3361 aagccgccgg ggtggatttc ctcttttcac cgcttcagac cgctcctgcc aaatgccgtg
     3421 tcccgctggt tctctacgtc atggatctgc agtttatcgt cgagccactg gcccggccgc
     3481 gctggcgcaa caaggctgcc gtcaaacgcg tcatgcagag ctgcgaaacc gcaactgcta
     3541 tcctcgcgtc ttccgaattc attcgcaggc agctcctcga actcctcgat gtgcccctcg
     3601 acaaagtgat cgtcgcgccg ctgggcgtct ccgatgtctt cgcggaaccg caggcctgct
     3661 gggtgcagca accctttttc ctgcatgtgg gcgcgacgcg cgcgtcgaaa aatatcgaga
     3721 gcctcctgag tgccttcgag atgtttgaag atcgtacccc ccacagcctc gtgatggtcg
     3781 gcctgccggg agatcgcgag caggcggatt ggggtccccg gatcatgcgt attcaccaat
     3841 gccccgagtc cgctctggcc ggcctctacc agcactgcga cgccctcgtc tgcgcctcgc
     3901 tttatgaggg tgcgggcgtc accgtcctgg aaggcatgcg ggcgggcgcg cagatcgtcg
     3961 cggggcgcgt cggagccatc ccggaattcg ccggaaatgc ccccatctac tgcaatgccc
     4021 agaacgcgtc caccatcgcc gccgccatgc agcgcgccct cgagctcgac cccgacaaac
     4081 gagagcgccg cgccatgtac atgacccatg ccgccgccga atatacctgg gacaagtgcg
     4141 cctggaaaac gcttcaggct ttccgggcgc gttgacggga ctctcccggc aatttggagc
     4201 ggagtccctc aatttcccag cgtgtgccgg gtcagctata atgcagttcc ttcagcttct
     4261 ttgggaatct gactcacttg gagtgatgcc atgactgttt cacatcgaac gcgttccgcc
     4321 ctgtggattg ttctcttgct ggctgcgtgg agtggcggca gcgccgtttc ccaggaaaat
     4381 tccccggcgg ccgtccctct ggaccctgcg accgctacca cggagttcgg cgaaggcctg
     4441 ctctggtacg acgcggcaaa ccttcccatc gacggcaagg gatggaacga cgtcgccgcc
     4501 tgctatgatc ggctcccggc ccgcgccgaa ggcgtggtac gcccgcctgt ctggaacctc
     4561 agccgcgact ccgcgggcct ggccatccgg tttcagaccg attcgcccgc aatcgccgcg
     4621 cgctgggtcc tccgcgactc gaacctggcc atgccgcata tgcctgccac gggcgtgagc
     4681 ggcctggacc tgtacgtgaa actcgccgat cgctggatgt gggtggctaa cggccgcccg
     4741 tcgcaggtga cgatgcaggc cagcctggtc acgggcatcc cggaaggcat gcgcgaatac
     4801 ctcctgtacc tgccgctgta caacggcgtg accagcgtcc atatcggcat caatccagat
     4861 tcagttctgg ccgcgccacc cgagcggtcc gaaaccacgc gcagccctgt tttgttctgg
     4921 ggcacctcca tcacgcaggg cggctgcgcc tcccgtcccg gcatggccta tccgtccatc
     4981 gtcggccgca tgatggatcg cccaaccatc aatctcggat tctcgggcaa tggaaagctg
     5041 gagcccgaga tggccgcttt tattgccgaa cttgacgtgg cggtgtttgt tctcgattgc
     5101 tgccccaatc tgaaacccga ggaaattgcc gagcgcacgg aaccggtcgt ccaggtgctt
     5161 cgtgaagccc atccggacac gcccatcgtt ctcatggaga acatcatcta ccagagtggc
     5221 tattttctgc cgggcacgcg cagggcgtat gaggcaaaga atgccgaact gaaggcggcg
     5281 tacgagcggc tgactgctgc aggcgtcacg aatctgcact atatcccgtg cgacaatctg
     5341 ctcggcgacg atgcggaagc cacggtcgat ggcactcatg cgacggatct gggattccag
     5401 cgcatggcgg aggcggtcac accgatcctc aagtctctgg caggcgaata agctcgccgg
     5461 agcgccggcc gagaaagggc tttgcaccat gttgaacgaa ctcgagcttt tccgggtccg
     5521 catcgggctc ctgcaggtgt tcctgaaaga gcgccaatat gacggcgttc tcctgtcgcg
     5581 cactgacaat tttgcgatgg ccaccgcggg caaacgcaac tatgtgtccc tgtgctccga
     5641 tctgggcgcc agttctctgt atgttacgca ggccggcgaa gcgtacttcg tgggaaacaa
     5701 catcgaggaa acccgtgtgg ttgccgaaga actgcgtgcc agcggatgcg aaatccgaaa
     5761 attcatgtgg ttcgaagact ctgcaccggc cctcgttcag cgtgaattct ccggcgccat
     5821 cgtctcagac gacggcagcc tcggcaaaaa cgtcaacggc gaaatggcgt acattcgctc
     5881 gctgctgacg cccaatgagc tggaaaaata ccgccatctc ggcaaactgg ccgcggaaac
     5941 catcaccacc gccgtagagc agaccgaagc cgggatggcc gaagccgata tcgccgcgat
     6001 cctcattggg gaaggcgcca aacgccgttg ccaggtcccc attgccctga tcgccgctga
     6061 ccgccgcgcc accaaatacc gccatccgct tcccaccgtc gcacctctgc tcccggagag
     6121 aatgcgcgag atccaggtgc gcgggtacgt gatgatcatt ctgtccctgc tgaaagaggg
     6181 gctcgtggtg tccgccaccc ggtttaaacg cgtgggcgac ctgtccgaac gcatcccctc
     6241 ggcctacgac cgcatctgcg gcgttgatgc gctgatgtac gaagccaccg agccgggcaa
     6301 aaccctcggc caggtctttg cggattgcca gcaagcctat aacgccatgg acttcgcgcc
     6361 caccgaatgg cacaaccatc accagggcgg caccaccggc tatgcaggcc gcacgtgcaa
     6421 agcttcgccc agcgagccgt tcctcatctt cgactcatcg tgggaagaca aggtgcgcga
     6481 catcacgggc atcgacgtcg cattcggtca ggcgttcgcc tggaatccct ccgctcccgg
     6541 cgtcaaatct gaggacacct tcctcctcat gcctgacggc accaaggaga tcatcacgcg
     6601 cacgccggcg cttccccgcg tcgatttgac cgccgtgctt ggccggaaaa ccgaggccgt
     6661 caagagcggt atcgccgaac cgggagcatg accgcacaga ccggcttcat cacggtgatt
     6721 tcatcagacc actgcgccgc cggcggtagg agatcccgcg acgcccgcgg cccgtggcct
     6781 cacccggcgc ctggtacagg gagatgaccg gcacggcttc ctcccaatcc gcgcgcggca
     6841 gattctttcc aagcgcgcgt tcgatcgcct ccagcgcaat atggtccgtg ggggacacaa
     6901 acgtgatggc atccccatcg ctgttggccc gggccgtgcg cccaatccgg tgaatgtaat
     6961 cgtccggatt ctccggaata tcaaaattga tcacatgact gacgttttcg atgtccagcc
     7021 cccgggccgc gacgtccgtt gccaccagat accggtattt gcccgagcgg aaccccgcca
     7081 acgcctcatc gcgctgacgc tgcgaccggt ctccatggat ctcctgcacc ttgaacccgt
     7141 tccggcgcaa ggctctggtg acccgttccg tgcgcggctt cgtgcgcagg aagatcatcg
     7201 cgctgtccac ctgctgttca cgcagcacct tcgccagcag tgcgatcttg cgttcgggat
     7261 tgaccgtata cactacctgc cgcactgtat cgaccggctg cgccaccagg cccacttcga
     7321 tccgttccgg atcattcaaa aattcccggg acaggcgcgc gatctccgga ggaaatgtcg
     7381 ccgaacacat cagcgtctgc cgcgcttgcg gaagtttctg gatgatgctc cggatgtccg
     7441 gcagaaaccc catgtcgagc atccggtccg
//
LOCUS       DAOPBQ010000115         7417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9018_length_7417_cov_3.333198, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000115 DAOPBQ010000000
VERSION     DAOPBQ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9018_length_7417_cov_3.333198"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1911
                     /locus_tag="PKW60_05320"
     CDS             <1..1911
                     /locus_tag="PKW60_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPJ98884.1"
                     /translation="ALFEMYYKAGFTAVWWQMMMLIAMVLVSISGWVQYRYRQTRSMT
                     MAQFFEVRYSRSFRVFAGLLAFVSGSINFAIFPAVGGRFFQYFCGFPPYVVSVGILEI
                     DLVFAGIMLFLLTIALAFTLMGGQVVVMVTDFIQGTFVNIMFVVLGLFLLLHFNWATI
                     EEALSMAPESASMIHPLKTADTENFNLTYFLIAVFGIVFTFMAWQGNQGYYCAAKSPH
                     EAKMGRLIANLRQITQSLTMILLPISAYVLLHHPSFSGYAENVNRVLDTVPSEQLRSQ
                     LTVTVALAHFFPVGLAGAFAAVMFAAFVTTHDTYLHTWGSIFIQDVVLPVRNTLRGEI
                     SPLGPKQHIRWLRVSILGVAVFIFLFSLLFNQQQDILMYFALTGTIYLGWAGAAIIGG
                     LYWKRGNTAGAWAAAVTGMTLAVAGWYMTYFWPNTQALVSNLLPGLWERALGWWPKLG
                     GEKCPITSQVLWFWTMVASAVVYAAVSLATGRGRVSDMDRILHRGKYTREGVANSATG
                     LRALAMGSEYSFTDRCLVIGSYGYSFLFLIIFIAGTLYSLSTPVKDSTWMAAWRVYCY
                     FMFALSVIITVWLAIGGIHNLKELLAALRVAERDDRDDGTVVGNRNLDDLDDEEFARM
                     NIAEPELAEDSK"
     gene            1908..2657
                     /locus_tag="PKW60_05325"
     CDS             1908..2657
                     /locus_tag="PKW60_05325"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPJ98885.1"
                     /translation="MRFGRITVAVLALAAVAALAGMQSDTAPVMVAKFTGTPVVVDGR
                     LDDAVWETATAYPLSLGRDSEAKGETLHEAGEVRLAWDNEFLYVGVKYTDSDIVAEGK
                     NDQEHHYLTGDLAEVFLKPRASTWYWELYVTPHEKKTHLWFPGSGRMGLKSNEEFSME
                     LHVAAQCQGTLNDWRDRDTSWTGEMAIPIRELTRFGDKFGPHADWTILVARYNYSRYL
                     PLRGPELSMAPQLPVTSYHYHDGYASLRLER"
     gene            complement(2754..3383)
                     /locus_tag="PKW60_05330"
     CDS             complement(2754..3383)
                     /locus_tag="PKW60_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPJ98886.1"
                     /translation="MRLAVNPTRMELLRLRKRLTVARRGHKLLKDKLDGLMKDFGKYA
                     NAYKERRLAVDKQLPEVLKHFVLAEATSSRAVIEDALDLTRQELYVDAQLRRVMGVAI
                     PRIDVTFGEAQGGYSLMHTSPELDSAIDSLKKFIPELLHMSELEQTVRLLSQEIEKTR
                     RRVNALEHTLIPNMAETSKYIKSKLDEIERSTTSQLMKIKADRMTDGAM"
     gene            complement(3426..4802)
                     /locus_tag="PKW60_05335"
     CDS             complement(3426..4802)
                     /locus_tag="PKW60_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPJ98887.1"
                     /translation="MVTREYRTAKNIIGPLILVEGVEGISYGELADIKTAGEDGLRRG
                     KVLEVNGDKAVVQVFEGTSGLAPQALSVKFLGKSQEIGVSADMLGRVFNGAGRPIDDG
                     PAIIPEKWLNVNGSPMNPYARDYPNEFIQTGISTIDLLNTLVRGQKLPIFSASGLPHS
                     RMAAQIARQARVLKTGERFAVIFAAMGITFEESEFFIADFRRTGAIERAVLFMNLADD
                     PAIERISIPRMALTTAEFLAYEKGMHVLVILTDLTNYCEALREISSARKEVPGRRGYP
                     GYLYTDLSSIYERAGRIKGKPGSITQFPVLTMPEDDKTHPIPDLTGYITEGQIILSRP
                     IHAQGIYPPVDVLPSLSRLKDKGIGKGKTREDHADVMNQLYSAYARGKSAKELAVVLG
                     ESALSEEDLLFVKFADAFEGKFIRQGEDENRTIEESLAIGWDLLSLLPRESLKRVRDE
                     FIDKYYPK"
     gene            complement(4852..6609)
                     /locus_tag="PKW60_05340"
     CDS             complement(4852..6609)
                     /locus_tag="PKW60_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPJ98888.1"
                     /translation="MNNNQGEVVKVSGPLVVARGLAGARMYEMVRVGEMRLFGEVIEI
                     RGDDCSIQVYEETEGIGPGQPVFRTGEALSVELGPGLIRSIYDGVQRPLNDLNVKFGD
                     FIVRGAESPALSRETQWEFEPAVKTGEKVAPGDVLGTVQESKLVIHRIMVPAGVEGKI
                     TRIGPVTGNVDTRVAVIETESGPVDVTMVQRWPVRMGRPVKRKLPPTVPLTTGQRVID
                     MFFPMTQGGTACVPGPFGSGKTVVQHQLAKWANAQIIVYVGCGERGNEMTDVLMEFPE
                     LKDPQSGEPLMERTVLIANTSNMPVAAREASVFTGITIAEYFRDMGYSVALMADSTSR
                     WAEAMREMSGRLEEMPGEEGYPAYLGSRIASFYERAGNVICLGNDDRAGSLSLIGAVS
                     PPGGDFSEPVVQATLRVVKVFWGLDDKLAFARHFPAINWLTSYSLYQEAVDVYAGEHL
                     GAGWQDARQRAMGILQREADLDELVRLVGMDALPVDDRLLMQAAKMIREDFLHQNAFD
                     DRDTYTSLPKQFQLLSLILHFYDVAQPALSQGVPLNALQGLEELEQVARAKLIPEDNL
                     DEFDRLKERITQAVAALPR"
     gene            complement(6666..6980)
                     /locus_tag="PKW60_05345"
     CDS             complement(6666..6980)
                     /locus_tag="PKW60_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPJ98889.1"
                     /translation="MSKAVVVGEESLVLGFKGAGFEIVDVPDGEQLLTELNRLARDPA
                     VALVLVTESVAEQAPGALQQFREVSQAILTTIPTHLGSKHLGFKDMRRLVERSIGVDL
                     LG"
     gene            complement(6973..>7417)
                     /locus_tag="PKW60_05350"
     CDS             complement(6973..>7417)
                     /locus_tag="PKW60_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98890.1"
                     /translation="VLAWATERVLSKSVSALWRALRAGMNRKEFEQRFLPIDGITGIL
                     RDILEAGEPQTWGGVIPGPLGDAFRESLESPVEEQIARFEHLASNHLTELARQSKLQT
                     MGPERVSGFLWGLWVEAFNMKLVISGKLNHIDSEALRVRVRETYV"
BASE COUNT         1386 a         2190 c         2267 g         1574 t
ORIGIN      
        1 gccttgttcg agatgtacta caaggcggga ttcacggcgg tgtggtggca gatgatgatg
       61 ctgatcgcca tggtgctcgt ctccatttcc ggctgggtgc agtaccggta ccggcagacc
      121 cgcagcatga ccatggccca gtttttcgag gtgcggtaca gccggagctt ccgggtcttt
      181 gcggggctgc ttgcgtttgt gtcgggcagc atcaattttg caatattccc ggcggtggga
      241 ggccggtttt ttcaatattt ctgcggtttt cctccgtacg tagtcagcgt gggaatcctg
      301 gagatcgacc tggtgttcgc gggcattatg ctctttttgc tgacgatcgc gctggcgttc
      361 acgctgatgg gcggccaggt tgtggtcatg gtgacggact tcatccaggg cacattcgtc
      421 aacatcatgt tcgttgtgct gggccttttc ctgctgctcc actttaactg ggcaacgatc
      481 gaggaagccc tgtcgatggc gccggagagc gcctcgatga ttcacccgct caagaccgcc
      541 gataccgaga attttaacct cacctacttt ctcatcgcgg tgtttgggat cgtctttacc
      601 ttcatggcct ggcagggcaa ccaaggttac tattgcgcgg caaaatcgcc gcacgaagcc
      661 aagatgggcc gcctgatcgc caatctgcgg cagatcacgc aatcgctgac catgattctg
      721 ttaccgatat ccgcgtacgt gcttttgcac cacccctcgt tttcaggcta cgccgaaaac
      781 gtcaaccgcg tacttgatac tgtgccaagc gagcagctgc gctcccagct tacggtgacg
      841 gtcgcgctgg cgcacttttt cccggtaggg ctcgcgggcg ccttcgcagc cgtgatgttt
      901 gcggcgttcg tgaccacgca cgatacctac cttcacacct ggggcagcat tttcattcag
      961 gatgttgtgc tgccggttcg aaacacgctg cgcggggaga tctcgcccct gggacccaaa
     1021 cagcacatcc gctggcttcg cgtatcgatc ctcggggtgg cggtattcat ttttctcttc
     1081 agcctgctgt tcaatcaaca gcaggacatc ctcatgtatt ttgcgttgac gggcacgatt
     1141 tatctgggtt gggccggcgc cgccatcatt ggtggcctgt actggaaacg gggcaacacg
     1201 gccggcgcct gggcagccgc ggtaacgggc atgacgctgg cggtcgccgg ctggtacatg
     1261 acatatttct ggccgaacac gcaggctctt gtgtcgaacc tgctgcccgg attgtgggag
     1321 cgcgcgctcg gctggtggcc aaaactgggc ggagagaaat gcccgatcac gtcgcaggtg
     1381 ctgtggtttt ggacgatggt cgcctcggcc gtggtttatg ccgccgtgtc gctggcaacg
     1441 ggccggggcc gcgtatccga catggaccgg atcctgcacc ggggcaagta tacgcgcgaa
     1501 ggcgtggcca attctgcgac gggcctgcgg gccctggcaa tgggttccga gtattccttc
     1561 acggatcgct gtcttgtcat cggttcgtac gggtattcgt ttctgttttt gatcatcttt
     1621 atcgcaggga cgctgtactc gctgagcaca ccggtgaaag acagcacctg gatggccgcc
     1681 tggcgcgtgt attgctattt catgtttgcg ctatcggtga tcattacggt ctggctggca
     1741 atcggcggca tccacaactt gaaggagctt ctcgcggccc tgcgcgtagc cgagcgcgat
     1801 gatcgcgatg acggcacagt ggtggggaac cgcaatctgg atgatttgga cgatgaagaa
     1861 ttcgcgcgga tgaacattgc cgaaccggaa ttggcggagg attcgaaatg agattcggtc
     1921 gcattaccgt agcggtgttg gctctcgccg ccgtggcggc gctggcaggg atgcaatctg
     1981 acacggctcc cgttatggtg gcgaaattca ccgggacgcc ggtcgtggtt gacggcaggc
     2041 tcgatgatgc cgtctgggaa accgccacgg cgtacccgct ttccctcgga cgtgacagcg
     2101 aggccaaagg agagacgctg cacgaggcgg gtgaagtccg gctcgcctgg gataacgagt
     2161 ttctgtatgt cggggtgaag tataccgatt cagacatcgt ggccgaggga aagaacgacc
     2221 aggagcatca ctacctgacg ggcgacctgg ccgaggtctt cttgaagccc agggcaagca
     2281 cctggtattg ggaattgtac gtaacgccgc acgaaaagaa gacgcatctg tggttccccg
     2341 gcagcggacg catgggcctc aagagcaacg aagagttttc gatggaattg cacgtggctg
     2401 cacaatgcca gggcacgttg aacgactggc gggatcggga taccagctgg acgggcgaaa
     2461 tggccatacc gatccgcgaa ttgacccgtt tcggggataa atttggcccg catgcggact
     2521 ggaccattct cgtggctcga tacaactact cgcgatacct gcccttgcgc ggccctgaac
     2581 tgagcatggc cccgcaactc ccggtcacga gctaccatta ccacgacggg tacgcatcgc
     2641 tccggcttga acgctgagcg gaggagccgc cgcctccgga ggatgaatac cgctccgctc
     2701 gaacgaggaa gcgcgccgca gcacgtctgg agacagccgt cccgggggta cggctacatg
     2761 gcgccatcag tcatccggtc ggccttgatc ttcatgagct ggctggtggt ggaacgctcg
     2821 atttcatcca atttgctttt gatgtacttg ctggtctcgg ccatgttcgg gatgagggta
     2881 tgttcgaggg cgttcacgcg gcgccgcgtt ttttcaatct cctggctcaa gagacgcacg
     2941 gtttgctcaa gttcgctcat gtgaagcagt tccgggatga atttcttgag actgtcgatg
     3001 gcgctgtcga gttcaggcga ggtgtgcatc agcgaatatc cgccctgagc ctctccaaac
     3061 gtgacatcaa tgcggggaat ggccaccccc atgacgcgcc gcaattgggc atcgacatag
     3121 agttcctgac gggtcagatc cagggcatct tcgatcacgg cccgcgacga agtggcttcg
     3181 gcgaggacga aatgcttgag cacctcaggc agctgcttgt caacggccag ccggcgctcc
     3241 ttataggcat tggcgtattt cccaaagtcc ttcatgaggc cgtcgagctt gtctttcagg
     3301 agcttgtggc cgcgcctcgc gacagtgagc cgcttgcgga gacgcaggag ttccatgcgc
     3361 gttgggttta cggcgagacg cacggctgag ggatcctcct tttccaaacc atccaggccc
     3421 ggggcctatt tgggataata tttgtcaatg aactcatcgc gaacccgctt caatgattcg
     3481 cgcggcaaga gggagagcag atcccatccg atggccaggc tctcctcgat ggtgcggttt
     3541 tcatcctcgc cctgccggat aaacttgccc tcaaacgcat cggcaaactt gacgaacagg
     3601 aggtcttcct cgctcaaggc cgattcgccc agcaccaccg caagctcttt ggcgctcttg
     3661 ccacgggcat aggcggaata gagttgattc atcacatccg catgatcctc gcgcgtcttc
     3721 cctttaccga tacccttgtc tttcaggcgc gacagggacg gcaggacgtc gacgggcgga
     3781 tagatcccct gtgcatggat gggccggctg agaatgatct gcccctccgt gatgtagccg
     3841 gtcaaatccg ggatgggatg cgtcttgtcg tcttcgggca tcgtcaatac cggaaactgg
     3901 gtaatcgagc cgggcttgcc tttaatacgg ccggcgcgct cgtagattga cgagaggtcg
     3961 gtgtagaggt agccgggata gccgcggcgt ccgggcactt ctttgcgggc ggaggagatc
     4021 tcacgcaggg cttcgcagta gttcgtcagg tctgtcagga tgacgaggac gtgcatgcct
     4081 ttttcatacg cgagaaactc cgcggtggtc aaggccatcc gggggatgga gatgcgctcg
     4141 atcgcgggat catcggccag gttcatgaac agcacggcgc gctcgatcgc cccagtccgg
     4201 cggaagtcgg cgatgaaaaa ctcggattct tcaaaggtga tgcccatggc cgcgaaaatg
     4261 accgcaaacc gctcgccggt cttcaaaacc cgggcctggc gtgcgatctg ggcggccatg
     4321 cgggaatgcg gcagtccgga ggcagaaaag atgggcagtt tctggccacg aaccagcgta
     4381 ttcagcaggt cgatcgtgga gatcccggtt tgaatgaact cattgggata gtcgcgggca
     4441 tacgggttca tcgggctccc gttgacgttg agccatttct cggggatgat ggccggcccg
     4501 tcgtcgatgg gacgcccggc gccgttgaac acccggccga gcatgtctgc cgagacgccg
     4561 atctcctggc tttttccgag gaacttgacg gatagagcct gcggggcgag tccgctggta
     4621 ccctcgaaaa cctgaacgac ggctttgtcg ccgttaactt caagcacctt gccgcggcgc
     4681 aggccatcct ctccggcggt tttgatgtcg gccagttcgc cgtacgagat gccttcgacg
     4741 ccctcaacca ggatgagcgg cccgatgatg ttttttgctg ttctgtactc acgcgtgacc
     4801 atggtcgttc cgtgtaccct ttctttcgcc cttgcgcctt tcgcgccgcg gttaccgcgg
     4861 caacgccgct acagcctgtg taatgcgttc tttcaggcgg tcgaattcgt ccaggttgtc
     4921 ttcggggatc aattttgccc gggccacctg ctccagttcc tccagcccct gcagggcgtt
     4981 gagtggcacg ccctgcgaca aggccggctg cgccacgtcg tagaaatgca ggatgaggct
     5041 cagcagctgg aactgctttg gaagagacgt ataggtatcg cgatcgtcaa aggcgttctg
     5101 gtggaggaaa tcctcgcgaa tcatcttggc cgcctgcatg agcagccggt catccacggg
     5161 cagggcatcc atgccgacca ggcggaccag ttcgtccagg tcggcttccc gctgcagaat
     5221 gcccatagca cgctggcggg cgtcctgcca gcctgctccc aggtgttcgc ctgcatagac
     5281 atcgacggcc tcctggtaaa gggagtacga ggtcagccag ttgatggctg gaaaatgccg
     5341 ggcgaacgcg agtttgtcgt caagtcccca gaacactttc accacccgga gggtggcctg
     5401 cacgaccggt tccgagaaat cgccgccggg gggcgacacg gcgccgataa gcgagagcga
     5461 acccgcccgg tcgtcgttgc ccaggcagat gacattgccg gcccgttcat agaaactggc
     5521 gatgcgggag ccaaggtagg ccggatagcc ttcttcaccc ggcatttcct ccagccggcc
     5581 ggacatttcg cgcatggcct ccgcccagcg gctggtcgaa tcggccataa gggccaccga
     5641 ataccccatg tcccggaaat actcggcgat ggtgatgccg gtaaacacgc tggcttcacg
     5701 ggcggcgaca ggcatgttcg aggtgtttgc gatcagcacg gtgcgttcca tgaggggctc
     5761 accggactgg ggatctttca gctcggggaa ttccatcagc acgtcggtca tttcgttccc
     5821 gcgctcgccg catccgacgt acacgatgat ctgggcgttg gcccatttgg caagctggtg
     5881 ctgcaccacg gtcttgccgc tgccgaacgg gccggggacg caggcggtgc ccccttgcgt
     5941 catcgggaaa aacatgtcga tcacacgctg gccggtggtc aacggcacgg tgggaggcag
     6001 cttgcgtttg acgggccgtc ccatgcgtac cggccaccgc tgaaccatgg tgacgtcaac
     6061 cgggccgctt tcagtctcga tcacggcaac ccgcgtgtcg acattgccgg tgaccggccc
     6121 gatgcgcgtg atcttgccct cgacgcccgc agggaccatg attctgtgga taacgagctt
     6181 actttcctgt acggtgccga ggacatcgcc gggggcgact ttttcgccgg tttttacggc
     6241 cggttcgaat tcccactgcg tttcccggct caacgcgggg ctttcggccc cacggacgat
     6301 aaaatcgccg aatttaacgt tgagatcgtt caacgggcgc tggacaccgt cgtagatgga
     6361 gcggatcaag ccggggccca gttccacgct gagggcctcg cccgtgcgaa acaccggctg
     6421 ccccggcccg atgccttcgg tctcttcata gacctggatc gagcaatcgt cgccgcgaat
     6481 ttcgataacc tcgccaaaca ggcgcatctc gcccacgcgg accatttcgt acattcgggc
     6541 gccagccaat ccgcgagcga cgacaagagg cccggatact ttgaccactt caccctggtt
     6601 attgttcata atctccatcc tcgtaatacc ggattcgcta tgcgcagagc acgaatgcct
     6661 tatcttcagc ccaacaggtc caccccgatg gaacgctcga cgagccggcg catgtcttta
     6721 aagcccagat gtttactgcc gagatgcgtc gggatggttg tgagaatggc ttgactcact
     6781 tcccgaaact gctgcaacgc gccaggcgcc tgttccgcca cggattcggt cactagaacg
     6841 agcgccacgg ccggatcgcg ggcgagccgg ttcaactcgg tcagaagctg ctcaccgtcg
     6901 ggcacatcca cgatttcaaa tcccgcgccc ttgaagccga gcaccaggga ttcctcacct
     6961 accacaaccg ctttagacat aggtttccct cacgcggacg cggagcgcct cggaatcgat
     7021 gtggttcagc ttgccgctga tcaccagctt catattgaac gcctctaccc agagtcccca
     7081 caggaaacca gagacgcgtt ccggccccat cgtctgcagc ttgctctgac gggccagctc
     7141 agtcaggtgg ttggaggcga gatgttcgaa acgggcgatc tgctcctcca caggactttc
     7201 gagcgactcg cgaaacgcgt caccgagggg gccgggaata acgcctcccc aggtctgcgg
     7261 ctcgccggct tccagaatgt ccctcaagat gcccgtgatg ccatcgatcg gcagaaaacg
     7321 ctgctcgaat tccttccggt tcatgccggc tcgaagcgcg cgccacaggg cggaaacgga
     7381 cttgctgagt acgcgttccg tggcccacgc cagaacg
//
LOCUS       DAOPBQ010000116         7405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9041_length_7405_cov_4.259592, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000116 DAOPBQ010000000
VERSION     DAOPBQ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9041_length_7405_cov_4.259592"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1721)
                     /locus_tag="PKW60_05355"
     CDS             complement(96..1721)
                     /locus_tag="PKW60_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPJ98891.1"
                     /translation="MDALDAVIAYHEATKHHPGRFARSPGHLDWANQPEPFRRFEGAP
                     LHPLPLVTGDPTPPYDALFMGDIPAQPLTRDTVGSFFEHALGLSAWKEYRGERWALRC
                     NPSSGNLHPTEGYALLPPISGIHDGPAVYHYAPREHALERRAELTPDAWSALAAALPE
                     NAFLVGLSSIHWREAWKYGERAFRYCQHDLGHAFAALAISAAALGWSMRRLDTPADTE
                     LASLLGLARLDDFPPGEHEHPGLVAAVFPGATTATAFDVPPEVFADAAAAWHGRAKPL
                     STGHVPWAAIDDVARATAKPRTAPQPPQLPPRPVPTTHPVTSSARTIIRRRRSAIAMD
                     GHTACPCDVFFRILARLLPDRAGPIWSAFAATPQAALIFFVHRVKNLQPGLYALVRNP
                     EHEPLLRDAMRSDFLWSKPAECPEDLPFYLLTPGDCRRLAGQLCCGQAIASDGAFAAA
                     MLAAFDHPLRHVGPWAYRVLFQETGMLGQLLYLEAEAAGLRGTGLGCFFDDLVHEVLG
                     LRDHQFQSLYHFTVGGFVDDPRLSTLPPYPERE"
     gene            complement(1805..2566)
                     /locus_tag="PKW60_05360"
     CDS             complement(1805..2566)
                     /locus_tag="PKW60_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98892.1"
                     /translation="MPMRRLFAAAAISAFAAPLFAGESPENVLRTAENQVVTAWKSVQ
                     AARGVLHATVQVDLQGLTLPIEASGPFAFKNHEGKRLFRVDLEGDISAPAVSPTGALP
                     VQGVGVFDGSIAHFMTNMLFQKTVMRYRPEEQDLAIGGDALFKALHENCELRLLPERT
                     LDKVPCHVFEAVVREPGDDMLEPVKWTLFFAKDTGLPLYVQALDRRNVKVFSCYMTGV
                     VLNPELNLSRFNFVPPPGARVVEGDDFSQLIPIPR"
     gene            complement(2667..5507)
                     /locus_tag="PKW60_05365"
     CDS             complement(2667..5507)
                     /locus_tag="PKW60_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPJ98893.1"
                     /translation="MMSQSQTGTLRSAQCEIRTDDLTRALYATDASIYQIRPEAVAFP
                     KSAQEIVSALQAAAEAGLSVTPRGAGTGLAGGAIGDGLIIDFARYNRRISDFDRDKQT
                     VRVQTGVILDQLNAFLKPQGFCFGPDVATSSRATLGGMIANNSSGARAPIHGTTAEHI
                     VALDVILADGRIETVGADSPALAAENARIRQLVEQHEALIERWFPNILVKRWPGYGLD
                     RYLRAPGDLCKIISGSEGTLAGIFAAELRVVPLPTDITGIAIFFFDSVTEALQASVEF
                     ADLEPAAIEHADRLLFDQTRGQLAFKAARDLLRLDEEPCEAFLIVEFQRDAEARMAEA
                     SRRNVGSRRMTVTDPAQMERIWNMRKAGLSLLTGRKGPAKPIPGIEDVAVEPARLPAY
                     VKELQAIMKDLGLVGSFYGHAASGLLHVRPLLDLHRSDDIARLRLLAEQTSALARRYN
                     ASFTGEHGVGIARTEFMAEQTGAELLAVMGEIKSLFDPQGLLNPGKIIAGNHDFRIDT
                     RLRLGGGYELSLPFPPRLAFADKDASFVGNLEQCNGCGGCIKNAPTMCPTYIATGEEL
                     MSTRGRANTIRAVLEHRFDDVDLLACTQLDEALSNCLSCKACKKECPSNVDMALLKAE
                     LLHALQQKSGIGLRERMVSSIETMNILGCLAPRIANAAMQWKWLRKLMELTLGFAAQR
                     PLPKFALERFDVWFAKHKRDAKYPATRGRVVLWDDTFVRYNEPHIGIAAVAVLEAAGF
                     EVVLPRGRRDCGRPAFSVGRLDMAKRLGTHNVELLRDRYPELPVVFLEPSSFAMFKQD
                     YRELCVPNAADLVPRCSLFEQFLFDVLEDDPGALAWNGGIGHVVIHAHCHAKALTDPS
                     VQQKLAAKIPGAQVTLLDSGCCGMAGQFGALSTKYELSLQVAEPLVRMINERPEGALV
                     VASGTSCRQQITHLTDVQPLHMAELIARAIAK"
     gene            5708..6703
                     /locus_tag="PKW60_05370"
     CDS             5708..6703
                     /locus_tag="PKW60_05370"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase"
                     /protein_id="HPJ98894.1"
                     /translation="MINHSNPEIQFAMEAVRAGALLAREVEREMVEPALTKEDRSPVT
                     VADFAVQGLIACLLEKTFPTAALVAEEDSAQLREPEAESTCRQVAAYVGRRLAYATPE
                     TVCAWIDHGNAAPRGLFWTLDPIDGTKGFLRGGQYAVGMALVDEGEIVLGAMACPNLS
                     DAHRPEPGGPGTLVIAARGQGAWWQPLETGGELFPLRVSERDDMSQMRILRSFETGHT
                     NVGRIERMAAAVGVTVEPVRMDSQAKYAVLASGHAELLLRLLTAKQPDYRERIWDQAA
                     GALITEEAGGRITDLDGKRLDFSAGRMLVNNRGVCASNGRVHDEVLDTLRAVCGE"
     gene            complement(6746..>7405)
                     /locus_tag="PKW60_05375"
     CDS             complement(6746..>7405)
                     /locus_tag="PKW60_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPJ98895.1"
                     /translation="TKTVSGKYWQLYYEIQDSNGERNEAISVADILENYKSAALEKGG
                     NIEYENLHNGFLTFTLPREEGGLTWCYIEASPGHYGLWIIDEKDFEKKLTFSTAEQMK
                     NTLDAEGHVAVYGILFDIDKAFLKLESIPPLQEIVKLLQQNASLKVEIQGHTDDQGSD
                     EHNLALSDKRAKTVLQYLTLFGIAPERLTAKGYGETMPVASNDTPEGRAKNRRVELVK
                     R"
BASE COUNT         1347 a         2074 c         2634 g         1350 t
ORIGIN      
        1 catccggcga ggcggggtga gggggcaatc gcccggcgcg ctgaaaatcc tgagggaatg
       61 gaagcgaagt cagagcaccg taaaattggc ccggctcact ctcgttcggg gtagggcggc
      121 agggtgctga gacggggatc gtcgacgaac ccgcctacgg tgaagtggta aagcgactgg
      181 aactggtggt cgcggagacc caggacctcg tggacgagat cgtcgaagaa gcatccgagc
      241 ccggtgccgc gaaggccggc ggcctcggct tcgaggtaga ggagttgacc gagcatgccg
      301 gtttcctgga agaggacgcg gtaggcccac gggcccacgt gccggagcgg gtggtcgaac
      361 gcggcgagca tggccgccgc aaacgcgccg tcagaggcga tggcctgccc gcaacagagt
      421 tgccccgcca ggcggcggca atcgcccggg gtcaagagat aaaacggcag atcttcggga
      481 cattcagcgg gtttgctcca gagaaaatcc gatcgcatgg catcgcggag gagcggttcg
      541 tgttcggggt tgcgtaccag ggcatagagt cccggctgca gatttttgac gcgatgcacg
      601 aagaaaatga gagcggcctg tggcgttgcg gcgaaggcgc tccagatcgg cccggcgcga
      661 tcgggcagga ggcgtgccag aatccggaaa aacacgtcgc agggacaggc cgtgtgtcca
      721 tccatggcaa tggcgcttct tcggcgccgg atgatggttc gtgccgaact ggtgacggga
      781 tgcgtggtgg gaaccggccg cggcgggagc tgcggtggct ggggcgcggt gcgaggcttg
      841 gcggtggccc gggccacgtc gtcgatggca gcccagggca catggcccgt gctcaacggc
      901 tttgcgcggc catgccaggc agcagcggcg tctgcgaaga cttcgggcgg gacgtcgaag
      961 gctgttgcgg tggttgcacc gggaaaaacg gccgcaacca ggccgggatg ctcgtgttct
     1021 ccgggcggaa agtcgtcgag gcgcgccaaa ccaagcaggc tggcgagttc cgtatcggcg
     1081 ggggtatcga gccggcgcat ggaccatccg agcgcggcgg cggatatggc cagagccgcg
     1141 aaggcatggc cgagatcgtg ctggcaatag cgaaacgccc gttcgccgta tttccaggct
     1201 tcgcgccagt gaatggagct cagcccgacc agaaacgcgt tttcgggcag cgccgcggcc
     1261 agggcgctcc aggcatcggg cgtgagctcg gcgcggcgtt cgagggcgtg ctcgcgcggt
     1321 gcgtaatgat aaacggccgg tccatcatga ataccggaga tcggcgggag cagcgcgtac
     1381 ccttctgtgg gatggagatt gccgcttgag ggattgcagc gcagggccca gcgctcacca
     1441 cggtactcct tccaggcgga aaggccgagg gcgtgttcga agaacgagcc caccgtgtcg
     1501 cgcgtgaggg gttgtgcggg gatatcaccc ataaaaaggg catcgtaggg cggcgtgggg
     1561 tcgcccgtca ccaatggaag cggatgcagc ggggcgcctt caaagcggcg gaacggttcg
     1621 ggctggttgg cccagtcgag gtgtcccgga gatcgggcga aacggccggg atggtgcttc
     1681 gtggcctcgt gatacgcgat gacggcgtca agggcgtcca tagtgcttgt cattgttgcg
     1741 gagagaggcg gacgcgtgca aggccggcca gcacaggcgg gcaaacgtgg cggctgccgt
     1801 cggctcagcg cggaatgggg atgagctgcg agaaatcgtc gccttcgacc acccgggcgc
     1861 ccggaggcgg cacgaaattg aagcgcgaca aattaagctc ggggttcaag acaacgccgg
     1921 tcatatagca gctgaagacc ttgacgttgc ggcgatcaag cgcctggacg tagagaggca
     1981 gtccggtgtc tttggcaaag aacagggtcc atttcaccgg ttcgagcatg tcgtcgccgg
     2041 gttcgcggac cacggcttcg aacacatggc agggcacctt gtcgagcgtg cgttccggga
     2101 ggagccggag ctcgcagttt tcgtgcaggg ccttgaacag ggcgtcgccg ccgatggcga
     2161 ggtcctgctc ttcgggccgg tagcgcatga cggtcttttg gaacagcatg ttggtcatga
     2221 aatgggcgat gctgccgtcg aatacaccga ccccctgcac gggcagggcg ccagtggggc
     2281 tgacggccgg tgcgctgatg tcgccttcga gatcgacgcg aaacaggcgt ttgccctcgt
     2341 ggtttttgaa ggcaaagggg ccggacgcct cgatggggag cgtcaatccc tggagatcaa
     2401 cctgcacggt ggcatggagc acgccacggg cggcctgcac ggatttccac gcggtaacga
     2461 cctgattttc agcggttctc agcacatttt caggcgattc cccggcaaac aagggggctg
     2521 caaacgcgct gatggcggcc gctgcgaaca agcgtctcat gggcatgaaa ttgtcctcaa
     2581 agcctgcatt ctccggctgg tagtgtgcca gatcggcgcg cgatgtgcca atgcgaaaag
     2641 cgtcattcgc ggaacgcggc cacgggtcat ttcgcgatgg cacgggcgat gagctcggcc
     2701 atatggaggg gttggacgtc ggtaaggtgg gtgatctgct gccggcaact ggtgccggag
     2761 gccaccacaa gcgcgccctc cggccgctcg ttgatcatgc gcacgagcgg ctcggcaacc
     2821 tggagagaca gctcgtattt cgtgctgagc gcgccgaatt gcccggccat gccgcaacag
     2881 ccggagtcga gcagcgtgac ctgggcgccc gggatttttg cggcgagctt ctgctgcacg
     2941 gacgggtcgg tgagcgcttt ggcgtggcaa tgggcatgga tgaccacatg gccgatgccg
     3001 ccgttccagg ccagagcgcc gggatcgtcc tcgagcacgt cgaagagaaa ctgctcgaag
     3061 agggagcagc gcggcaccag gtctgccgcg ttgggcacgc agagttcacg gtaatcctgt
     3121 ttgaacatgg caaacgatga cggttcaagg aaaacgacgg ggagctcagg ataacggtcg
     3181 cgaagcagct cgacattatg tgtgcccagc cgttttgcca tgtcgaggcg cccgacgctg
     3241 aacgcgggcc ggccgcagtc tctgcggccc cgcgggagga cgacctcgaa tccggccgcc
     3301 tcgagcacgg cgacggcggc gatcccgata tgcggctcgt tgtagcggac gaacgtgtcg
     3361 tcccagagaa caacgcgtcc gcgggtggcg ggatacttcg cgtcgcgctt gtgcttcgca
     3421 aaccacacgt caaagcgttc gagggcaaat ttcggaaggg gccgctgcgc cgcgaaaccg
     3481 agggtgagtt ccatgagttt gcggagccat ttccattgca ttgcggcatt cgcgatgcgt
     3541 ggggcaaggc aaccgagaat gttcatggtt tcgatcgaac tgaccatgcg ctcccgcagg
     3601 ccgatgcccg atttctgctg cagcgcgtgt agaagttccg ctttgagcag ggccatgtcg
     3661 acgttcgagg gacattcctt cttgcaggcc ttgcacgaga ggcaattgct gagcgcttcg
     3721 tcgagttgag tgcatgcgag aaggtcgacg tcgtcaaaac ggtgttcgag cacggcgcgg
     3781 atggtgttgg cgcggccgcg ggtggacatg agttcctcgc ccgtggcgat gtaggtcggg
     3841 cacatcgtgg gtgcattttt gatgcagccg ccgcacccgt tgcactgttc gagattgccg
     3901 acgaaagagg cgtctttgtc ggcgaatgca agcctgggcg ggaacggcag ggagagttcg
     3961 tagccgccac ccaggcgaag gcgcgtatca attcggaaat catggttacc ggcgataatc
     4021 ttgccggggt tcaagagccc ctgcgggtcg aagagcgact tgatctcgcc catgacggcc
     4081 agaagctcgg ctccggtctg ttcggccatg aattcggtgc gggcgatgcc gacgccgtgc
     4141 tcgccggtga aggaggcgtt gtatcgccgg gccagggcgg atgtctgttc ggcgagcagg
     4201 cggagtctgg cgatgtcgtc cgaccggtgc agatcgagca gggggcgcac gtgaagcagc
     4261 ccggatgcgg catggccgta gaatgatccc acgagaccga ggtccttcat gatcgcctgc
     4321 aactccttga cgtaggcggg gagacgtgcg ggctctaccg cgacatcttc gatgcccggg
     4381 atgggtttgg cgggaccttt ccggccggtg agcagggaaa ggccggcttt gcgcatgttc
     4441 cagatccgtt ccatctgggc cggatcggtg acggtcatgc ggcggctgcc gacattacgc
     4501 cgggaagctt cggccatacg cgcctctgca tcgcgctgaa attcgacgat cagaaaggct
     4561 tcacacggtt cctcatcgag acggagaagg tcgcgcgcgg ccttgaatgc gagctggccg
     4621 cgcgtctgat cgaagagaag gcggtcggcg tgctcgatgg ccgccggttc gagatcggca
     4681 aactcaaccg acgcctgcag ggcctccgtt acggaatcaa agaagaaaat cgcgatcccc
     4741 gtgatgtcgg ttggcagggg cacgacgcgc aattccgcgg cgaatatgcc ggcgagcgtg
     4801 ccttcgctgc cggaaatgat cttacagagg tcgccggggg cgcggagata acggtcaagt
     4861 ccgtaaccgg gccaccgttt caccagaatg ttgggaaacc accgttcaat gagggcttcg
     4921 tgctgctcga caagctggcg gatgcgggcg ttttccgcgg caagcgccgg agagtcggcg
     4981 ccaacggtct cgatgcggcc atcggcgaga atcacatcga gcgcgacgat atgttcggcg
     5041 gtggtgccgt ggattggtgc acgcgcgccg gaggaattgt tggcgatcat gccgccgagg
     5101 gtagctcgcg aactcgtggc gacgtcgggc ccgaagcaga atccctgggg tttaaggaac
     5161 gcgttgagct ggtcgaggat cacgccggtt tggacgcgca ccgtctgttt gtcgcgatca
     5221 aagtccgaga tacggcggtt atagcgggcg aaatcgataa tcaacccgtc cccgatcgca
     5281 ccgccggcca gcccggtgcc tgcgccgcgg ggcgtgacgc tgagccccgc ctcagcggcg
     5341 gcctggagcg ccgagacaat ttcctgggcg gatttcggaa aggcaacggc ttccgggcgt
     5401 atctgataga tcgacgcgtc ggttgcgtag agtgcgcggg tgagatcgtc ggtgcggatc
     5461 tcgcactgtg cggaacggag cgtgccggtt tgtgactgtg acatcatgag gtttttctat
     5521 gccgtttgtg taaccggggt ttgcatgctg tcgcgcggtt aatccgtccc ggtgcccgag
     5581 ccggcgcgcc tggcctgtgc cgacggaaca acaggcggaa gttttccgct gcttcatgcg
     5641 ttggcgagct tacgcgtggt atcatctcgc gtcaccaacg ccagggcatg aagggacagg
     5701 cggccttatg atcaatcatt cgaatccaga aatccagttt gcgatggaag ccgtgcgcgc
     5761 gggcgccctg ctggcgcggg aagtggagcg cgagatggtg gaaccggcgc tcacgaaaga
     5821 agatcggtcg ccggtgaccg tggccgactt cgcggttcag ggactcatcg cgtgcctgct
     5881 ggagaagacc ttccccacgg ccgcgctggt ggccgaggag gattcggcgc agttgcgcga
     5941 accggaggcg gaatccacat gccgtcaggt ggccgcgtac gtgggccggc ggctggcgta
     6001 tgcgaccccg gagacggtct gcgcctggat cgaccacgga aacgccgccc cgcgcgggct
     6061 gttctggacg ctggatccca tcgacggcac aaaagggttc ctgcgcggtg gccagtatgc
     6121 cgtgggcatg gctttggtgg atgaaggcga gatcgtgctt ggcgcaatgg cttgtcccaa
     6181 tttgagcgac gcccaccgcc cggagccggg gggacccggg acgctggtga ttgcggcgcg
     6241 tggccagggg gcgtggtggc aaccgctcga aaccgggggc gaattgtttc cgttgcgggt
     6301 gtccgagaga gatgatatgt cgcagatgcg catcctgcgc tcgttcgaaa cgggccacac
     6361 caacgtgggc cgtatcgagc ggatggcggc agccgttggc gtgacggtgg aacccgtgcg
     6421 catggacagc caggcgaagt atgcggtgct ggcgtccggc cacgcggagc ttcttctgcg
     6481 gctcctgacc gcgaaacagc ccgattaccg ggaacggatc tgggatcagg ctgcaggcgc
     6541 gctgattacc gaggaggccg ggggacgcat cacggacctg gacggcaagc gcctggactt
     6601 ttcggcaggg cggatgctcg tcaacaaccg gggggtctgc gcctcgaacg gccgcgttca
     6661 cgacgaggtg ctggacacgc tgcgcgcggt gtgcggagag taggctcctg cttacgcgca
     6721 cccgcggaat tcgtcgttca caaaatcagc gcttgaccaa ttcgacgcgg cggttctttg
     6781 cccttccctc gggcgtgtcg tttgaggcca ctggcattgt ctcgccgtac cccttggcgg
     6841 tcaggcgctc gggggcgatg ccgaacagcg tcagatactg cagcacggtc ttggcgcgct
     6901 tgtcggacag ggccagattg tgttcgtcgg agccctggtc gtcggtatgc ccttgaatct
     6961 cgaccttgag cgacgcgttc tgctgcagga gtttgacgat ttcctggagg ggcggaatgg
     7021 attcgagctt gaggaatgcc ttgtcgatgt cgaagagaat cccgtagacg gccacgtgcc
     7081 cctcggcatc gagagtgttc ttcatttgtt cagcggtgga gaaggtcagc tttttctcaa
     7141 aatctttttc gtcgataatc cagaggccgt aatgtcccgg ggaggcttcg atatagcacc
     7201 aggtcaggcc tccctcctct cgcggaagcg tgaaggtgag aaagccgttg tgcagattct
     7261 cgtactcgat gttcccgccc ttttcgagcg cggcactctt gtagttttcc aggatgtccg
     7321 cgaccgatat ggcttcgtta cgctcgccgt tggaatcctg aatctcgtaa tacaactgcc
     7381 agtatttgcc actgacggtc ttggt
//
LOCUS       DAOPBQ010000117         7397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9050_length_7397_cov_4.524925, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000117 DAOPBQ010000000
VERSION     DAOPBQ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9050_length_7397_cov_4.524925"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..883
                     /locus_tag="PKW60_05380"
     CDS             35..883
                     /locus_tag="PKW60_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPJ98896.1"
                     /translation="MTRRDGFTLVELLVVIAIIGILAAIVVPNVANWIGRARMARAVE
                     EIRGADLAMTKMLTDANVKDFRQFFTPASRGVYENLALAEQVYVCTYTSYLLLRQGRG
                     AMSNWDLKDPDPNRNIELSPEIVGRLGLNYMEGIGQDPWGKQYKFWFGPWRGARVLGA
                     AVTTSIPFRSWRMGDGDINDVSGYIPYVYDADMKLKAEAEVPGAPPADTRPGYPAPTK
                     MPIYIFSTGSNQNCDQDQLSLTGADKPLAFNDFTAGYTPDHEYDGGGDDINNWDSEAG
                     WAGFYS"
     gene            943..1842
                     /locus_tag="PKW60_05385"
     CDS             943..1842
                     /locus_tag="PKW60_05385"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPJ98897.1"
                     /translation="MKTLGTRMRGFTLVELLVVIAIITILAAVTIPTLVKSGAFQKEY
                     VEQSGRTVYNMLKAAEFYASTHNVDTCLAYAAETLPDSRDPSSFAKVVVARSVGVFRR
                     ATDEELAAWQSHYAGTPDEVLSEEFGTTIYTPVFVPVAEYELTKLGDGACVLGDWDTS
                     VDPPVHAPFWENVLGYPVKGLRPVRIRNDEGALVRLLPPHVPYLYEFEPFPGVLHSVD
                     DCWMAHVFSPAGYVDAPVTQERLMVTVGPTPDSAEDDRFINLAETPLDPSQLPPGYTS
                     NKFPVAPVKVQISKSSARVKIVE"
     gene            1853..2269
                     /locus_tag="PKW60_05390"
     CDS             1853..2269
                     /locus_tag="PKW60_05390"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPJ98898.1"
                     /translation="MKRQSGYSLLEVLIALAILMIGIIAVYRLFPMSMRAARLADEKI
                     VSSELASSRLGRLRSSGAQGPVVNFYTAPGAEIVDEHYTRVLSAAGDAYALYDGYTTI
                     LQRLRGASDIYYQRALFTVRMPDGRDETFVTYIVEQ"
     gene            2316..3575
                     /locus_tag="PKW60_05395"
     CDS             2316..3575
                     /locus_tag="PKW60_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPJ98899.1"
                     /translation="MMALQQLRSSKQRETMRGFTLPELLISIAIMSILTLALFMLFSS
                     TLKAFKSTKRSMEASEETHTAFRVMERDLAQAYSARHYGDSANFFGCPYGFSMIATLD
                     AAGQARTARISYVVHRFAGRKAVETLSDSADDALLPTFGIVRYIEPGVDKLSEYPVQW
                     PDYTSATPPVEQPQQHLYQPLREIYEQFGPNSDPSTMLFIAPDEYRPVSAAQLEAMLQ
                     AKKHELWLAMLGDRDEPYGELPRLWDDPAAPGTTWGGGPLFAGLWDGKYPEDYLIAEG
                     VVQADPEWRFPADYAITVNPGSQDKVEVQNFAEVHWFRYGYTPRDVDIIDDLGLVETV
                     PYWNAVDKANLGLAPDPVTGDPGEINFPFINLAGSPLYPFPPSLVEVNMAFAFPAPNL
                     GERHLVRKLAHTFQIPVGYRRPTLKQP"
     gene            3648..>7397
                     /locus_tag="PKW60_05400"
     CDS             3648..>7397
                     /locus_tag="PKW60_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98900.1"
                     /translation="MMRRSDRRGQALVVAIFVLAFLTAIAIAFYTSTTMQLRRAVNVT
                     NRVQAAQAAEAGIAMAQAFLRRDAMIHNTYTSYDFAFSTYFNGAAFAAKSWAFPPDPT
                     VPLTPPSAVPKIPGDIADALGDRLYIPRAQGNDPAVDAFDFTGVFAVNQAFGALTPAE
                     QIDAWADVDNNEDGLRDSVWLPVPLDVFFTDDGIDNNLDGLVDESPWMGEDGIDNTGD
                     GNIDDQDEIDLAEKVEPERAIFVYFGGNDGLDNNGNGLIDDPSEQKLFLTTRLPDGQG
                     GWLGDGPNFIINVRVNPDAPDFPEALLSQVQGVPPNTTIPVSNLNDVDVLDNDYDLVV
                     NNTGVYQFVDPSTDPSNPWTHIRGWDVLTREANMDHVLGAFNEIHTSYLTALGFPADA
                     HITILGEPVCRVVGRVAVLVEDATAKANLNIVNGYAPNYGQRGGGFNQDRMNSGGTAT
                     GPTIARNLGQGLDPSEWDARILPLSGYVKTGRLAQMRTGAPEGVGLRVDDAALPPSPM
                     AYDAGFPGYGRVDDNGNALWASISGIDWNGSGWPYDGVIPFDYIGAGSFQFPNLQGVD
                     EPQEFSPYRPRRNLVAENDGINNNEILNPGTKNELTDEFGELGDWYYRTKRQLELPSD
                     IGPATAVLWRPLTIAHSFSRNDRHQFFDSEGELLEEPEVSGLRLDYNYAFAAQIDAML
                     SRDWGYNVGTRLAPLGLPAEAAAFSLGLLRENVGVVPVGMSGNADPMPQGILGNLIGD
                     NGRFPADLGLRRLQLAANLVDRRDTDHAQTELTISVPDDWWNDRFSPPPPDEKRIEYT
                     AAGIESIRINELMVRPVRRVEAEMTVDPANPYYQALFKFNPADPPGYTTYLDPNGFSY
                     PDPRATDFLMLAENFETHLDYRNKIANYEAANGVSFAATTSGWNAGGNTTPLFLGKRA
                     YVYTPSRYLTNAPDTAYELGLTEPPPDVVQFLFAPSVQLPPGRYYLLISTLDGAGAPT
                     APEAWHLEYAVKYCVRNFTPPATPVYPNDYPVFGSGKRADWDDIIYDVFDQFYPANVA
                     APNPPVPWQQLDSYAMGDFIQRPDEAQLDGKIFLPTRLARNPAPVIPGYQQDEAYTVE
                     IPPLPLSGDPADQIFLCVAFKMGAAPPPGGAIALNFFEFSQEPDHEWIELVNIAEAPD
                     YADLGNPALMGATLSPAERERWAEMASVDVSGWQLEIGYEGNIGHTTMRIPKGTFLAP
                     GGSLLVAVNKYDYLESPDVVAPDPTDPPGLSPLIFQNGIGLAGGSV"
BASE COUNT         1445 a         2242 c         2341 g         1369 t
ORIGIN      
        1 cggagggcga tgcaacaacc aaggagtgtg agcgatgaca cgaagagacg ggtttacgct
       61 ggtcgagttg ctggtggtga ttgccatcat cggtatcctg gcagcgattg tggtgcccaa
      121 cgtggccaac tggatcggcc gcgcccgcat ggcccgcgcg gttgaggaaa tccgcggcgc
      181 cgacctggcc atgaccaaaa tgctgacgga cgcgaatgtg aaggacttcc ggcagttttt
      241 cactccggcg agccgcggtg tctatgagaa tctcgcgctc gcggagcagg tttatgtctg
      301 cacttatacc agctacctgc tgctgcgtca agggcggggc gcaatgagca actgggatct
      361 gaaggatccg gacccgaata ggaacattga gcttagcccc gaaattgtgg gccgcctggg
      421 cctcaactac atggaaggga tcggccagga tccctggggc aaacaataca aattctggtt
      481 cggcccctgg cgaggcgcgc gtgtgcttgg cgcagccgtt accacttcta tcccgtttcg
      541 ctcgtggcgg atgggcgacg gcgacatcaa tgatgtgagc ggctacatcc cgtacgtgta
      601 tgacgcggac atgaagctta aggccgaggc cgaagtgccc ggtgcgccgc ccgcggacac
      661 ccggcctggc tatcccgcgc cgacgaaaat gccgatctac attttctcaa ccgggagcaa
      721 ccagaactgc gatcaggatc agctcagcct gactggggcc gacaagccgc tggcgtttaa
      781 cgatttcacc gcggggtata cgcccgacca tgaatatgac ggcggtggcg acgacatcaa
      841 caactgggat tccgaggctg gctgggcggg cttctacagt taatttaacc gaggaccggg
      901 ggcggcgcgg cacgccgtcc ccggcgggaa cggattggaa cgatgaaaac gctgggcaca
      961 cggatgcgag ggttcacgct ggtcgaattg ctggtggtga tcgccatcat cacgatcctg
     1021 gcggcggtga ccatcccaac gctggttaaa tcgggagcgt ttcaaaaaga atacgtcgag
     1081 cagagcgggc ggacagtcta caacatgctg aaagccgctg aattctacgc gtcgacccac
     1141 aacgtcgaca cctgcctggc ctacgcagcg gagaccctgc ccgacagccg tgatccgagc
     1201 agctttgcca aggtggtggt ggcccgcagc gtgggcgtgt tccgccgagc gacagacgaa
     1261 gaactggcgg cctggcagag ccattacgcg ggtaccccgg acgaagtgct gagtgaagag
     1321 ttcggcacga ccatctacac gcccgtgttt gtgccggtgg cggaatacga gctgacgaag
     1381 ctgggggatg gcgcctgcgt gctcggcgac tgggatacga gcgtggatcc gccggtccat
     1441 gcgccgttct gggagaacgt gctgggctat cccgtcaaag gactgcggcc cgtgcggatt
     1501 cgcaacgacg aaggggcgct ggtgcgactc ctcccgccgc atgttccgta cctgtatgag
     1561 ttcgagccgt tcccgggagt ccttcactcg gtggacgatt gctggatggc gcacgtgttt
     1621 tccccggcgg gctatgtcga cgcgccggtg acgcaggagc gcctgatggt taccgtgggc
     1681 ccgacgcccg acagtgcgga agacgaccgg tttatcaatc tggctgagac gcccctggat
     1741 cccagccagc ttcctccggg atatacctcg aataaatttc cggtcgcccc ggtgaaggtg
     1801 cagatttcaa aatcgtcggc gcgtgtaaag attgtggaat gaggaggcag gcgtgaagag
     1861 gcaaagcggt tattcgttgc tcgaagtgct catcgcgttg gccatattga tgattggcat
     1921 cattgccgtg taccgcctgt tccccatgtc gatgcgtgct gcacgtttgg ccgacgaaaa
     1981 gatcgtgtcg tcggagctgg cgagttcccg cctgggccgg cttcgttcgt cgggcgcgca
     2041 gggtcctgtg gtgaatttct acacggcacc cggcgccgag atcgtagacg agcattacac
     2101 gagagtgttg tcggccgcgg gcgacgccta tgcgttgtac gatggttaca cgaccattct
     2161 gcaacggtta cggggcgcga gtgatattta ctatcagcgg gccttgttta ccgttcggat
     2221 gcctgacggc cgcgacgaaa ccttcgtgac gtacatcgtg gagcaatagg gagcgcgtct
     2281 ataccacacg cctcccgagg tggagagaac aggctatgat ggcccttcaa caattgaggt
     2341 ccagtaagca gcgcgagacc atgcggggat ttaccctgcc cgagctgctc atcagcatag
     2401 ccatcatgag catcctgacg ctggcgctgt tcatgctgtt cagctcgacg ctgaaggcgt
     2461 tcaaaagcac caagcgctcg atggaggcca gtgaggagac ccatacggcg ttccgggtga
     2521 tggagcgcga cctcgcgcag gcctattcgg ccaggcatta cggcgattcc gccaactttt
     2581 ttggctgccc ctacgggttc agcatgatcg ccaccctcga cgccgcgggc caggcccgca
     2641 ccgcccggat ctcgtacgtg gtccatcgct ttgccggacg caaagccgtg gagaccctga
     2701 gcgacagcgc tgacgatgcg ctacttccca ccttcggcat cgtccgctac atcgagccgg
     2761 gcgtcgacaa gctgagcgaa tatccggtgc aatggcccga ttacacgagc gcgacgccgc
     2821 ccgtcgagca gccgcaacag catctgtacc agccgttacg cgagatctac gaacagttcg
     2881 gccccaactc cgacccaagc accatgcttt ttatagcacc cgacgaatac cggccggtct
     2941 ccgcggcaca gctggaggcg atgcttcagg ccaaaaaaca cgaactgtgg ctggcgatgc
     3001 tgggcgaccg cgacgagccg tacggggagc tgccgcgttt gtgggacgat ccggccgcgc
     3061 cgggcaccac ctggggcggc ggcccgcttt tcgccgggct ctgggacgga aaatacccgg
     3121 aagactacct cattgccgaa ggagtcgtgc aggcggaccc ggaatggcgt tttcctgcgg
     3181 attatgcgat cacggtcaac ccgggcagtc aagacaaggt cgaggtccag aactttgccg
     3241 aagtccactg gttccggtac ggctacacgc cgcgcgatgt ggacataatc gatgacctgg
     3301 gcctggtgga gacggtgccg tactggaatg cggtcgacaa agcgaatctg gggcttgccc
     3361 cagatcctgt cacgggcgac cccggggaga tcaatttccc cttcatcaat ctggccggtt
     3421 ctccgctgta tccgtttccg ccgagcctgg tggaagtgaa catggccttt gcgttcccgg
     3481 cgccaaatct gggcgaacgt catctcgtcc ggaaactggc gcacacgttt cagataccgg
     3541 tgggataccg ccgtcccacg ttgaagcagc catagcaagc aagcgccgcg gccggtgccc
     3601 gtaagtcggg cgcggcgcgc ggcacaaggg aattgagaaa gggagttgtg atgcgcagaa
     3661 gcgacaggcg gggtcaggcc ctggtcgttg ccatctttgt gctggcgttt ctgacggcga
     3721 ttgccatcgc cttttataca agcacgacca tgcagctccg ccgcgccgtc aatgtgacca
     3781 accgggtgca ggcggcccag gcggccgagg cgggcatcgc catggcccag gcatttctgc
     3841 gccgcgatgc gatgattcac aacacgtata cgtcatacga cttcgcgttc agcacctatt
     3901 tcaacggagc ggcgtttgcc gcgaagtcct gggcgttccc gccggatccg accgtgcctt
     3961 tgactccgcc gtcggcggtg ccgaagatcc cgggcgatat cgcggacgct ctcggcgacc
     4021 gtctgtatat cccgagggcg cagggcaatg acccggcggt cgacgcgttc gattttaccg
     4081 gggtttttgc tgtgaaccag gcctttggcg cgctcactcc ggccgagcag atcgatgcgt
     4141 gggccgatgt cgacaacaat gaggacggcc tgcgcgattc ggtctggctg ccggttcccc
     4201 tggatgtatt ttttactgac gacggcatcg acaacaacct cgacggcctg gtcgatgaaa
     4261 gcccgtggat gggcgaagat gggatcgaca acaccggcga cgggaacatc gacgaccagg
     4321 acgaaattga tcttgcggag aaagtcgagc ccgagcgcgc gatcttcgtg tatttcggcg
     4381 gaaacgacgg cctggacaac aacggaaacg gcctgattga cgatcccagc gaacagaaac
     4441 tctttctcac cacccggctg cccgacggcc agggcggctg gctgggcgac ggccccaact
     4501 tcattatcaa tgtgcgggtg aatcccgatg cgcccgattt cccggaagcc ctcctatctc
     4561 aggttcaggg cgtgccgccg aacacgacca taccggtgag caacctgaac gacgtcgatg
     4621 tgcttgacaa cgattatgac ctcgtggtca acaacaccgg ggtctatcaa ttcgtcgacc
     4681 cgtccactga tcctagcaac ccttggacgc acatccgggg ctgggacgtg ctcacgcgtg
     4741 aagccaacat ggatcacgtc ctgggcgcct tcaatgaaat ccacacgtcc taccttacgg
     4801 cgctgggatt tccggctgat gcgcatatca cgatcctcgg cgagccggtc tgccgggtcg
     4861 tggggcgtgt ggcggtgctg gtggaagacg ccaccgccaa ggccaacctc aacatcgtca
     4921 atgggtatgc acccaattac ggtcaacggg gcgggggctt caaccaggat cgaatgaatt
     4981 ccggcgggac cgcgacggga ccgaccattg cgcgcaacct gggccaaggc ctcgatccga
     5041 gtgaatggga tgcgcggatc ctgccgctga gtgggtacgt caaaaccgga cggctggcac
     5101 agatgcggac cggcgcgccc gagggcgtcg gcctgagagt cgacgatgcc gcattgccgc
     5161 ccagtcctat ggcctacgat gcgggattcc cgggctatgg ccgggtagac gacaacggga
     5221 acgcgttgtg ggcatcgatc tcgggcatcg actggaacgg gagcggctgg ccttacgacg
     5281 gcgttatccc gtttgactac atcggcgcgg gcagcttcca gtttcccaat ctccagggtg
     5341 tggacgaacc gcaggaattc tcgccgtacc ggccgcgccg gaatctcgtg gccgaaaacg
     5401 acgggatcaa caacaatgaa atactcaatc ccggtacgaa aaacgagttg acagacgaat
     5461 ttggcgaact gggagactgg tactaccgga ccaagcgtca gctggagctg ccctcagaca
     5521 tcggcccggc caccgccgtg ttgtggcgcc cgttgacgat cgcgcacagt ttcagccgaa
     5581 acgaccgtca ccagtttttt gattcagagg gcgaactgct cgaagaaccg gaggtatccg
     5641 gccttcgcct cgattacaac tacgcgttcg ccgcacagat cgatgcgatg ctgtcccggg
     5701 actggggcta caatgtcggc acccggctgg cgccgctggg gcttcccgcg gaagcggcgg
     5761 ctttcagcct gggcttgctg cgcgagaacg tgggcgtagt gccggtaggg atgagcggga
     5821 atgccgatcc catgccgcag ggcattctcg gtaacctgat tggcgacaac gggcgtttcc
     5881 cggccgattt gggcttgcgc cggcttcaac tggccgcgaa cctcgtcgat cggcgcgata
     5941 ccgaccacgc gcaaacggag ctcaccatca gcgtgccgga tgactggtgg aatgaccgtt
     6001 tcagtccgcc gcctccagac gagaagcgca tcgaatacac ggcggcgggt atcgagagca
     6061 ttcgcatcaa cgagctgatg gtgcggccgg tgcgccgtgt tgaggcggaa atgacggtgg
     6121 acccggcaaa tccgtactac caggccctgt ttaaatttaa tcccgcggac ccgcccgggt
     6181 acaccacata ccttgatccg aacgggtttt cctatcccga tcccagggcg acggatttcc
     6241 tgatgctcgc ggagaatttt gaaacgcatc tggactatcg gaacaagatt gccaattacg
     6301 aggcggccaa cggggtttcg tttgcggcga ctacctccgg gtggaacgcc gggggaaaca
     6361 ccacgccgct cttcctgggc aagcgggcct atgtgtatac cccgtcccgc tatctcacga
     6421 atgcccccga cacggcatat gaactcggac ttaccgagcc gccgccggac gtggtgcagt
     6481 ttctcttcgc gcccagcgtg cagttgccgc cgggccgcta ctacctcctg atcagcacac
     6541 tggacggggc tggcgcgccc accgcgcccg aggcctggca tctcgaatac gcggtgaaat
     6601 actgcgttcg taacttcacg ccgcccgcta cgccggtcta tccgaatgac taccccgtgt
     6661 tcggctcggg caagcgcgcg gactgggacg acatcatcta cgacgtgttt gaccagttct
     6721 atcccgccaa cgtggccgcc ccgaacccgc ccgtgccgtg gcagcagctc gattcgtatg
     6781 ccatgggcga tttcatccag agaccggacg aggcgcagct cgacggcaag atcttcctgc
     6841 cgacccggct cgcgcggaac cccgcgcccg tgatcccggg ataccagcag gacgaggcct
     6901 atactgtcga aataccgccc ctgcccctgt cgggcgaccc ggcggaccag attttcctgt
     6961 gcgtggcgtt caagatgggg gccgcgccgc ctcctggtgg ggcgatcgcg ctcaatttct
     7021 tcgaattcag ccaggaaccc gatcacgagt ggatcgaact ggtgaacatc gccgaggcgc
     7081 cggattatgc ggatctcgga aaccccgccc tgatgggcgc gacgctgagc cctgcggagc
     7141 gcgaacgctg ggctgaaatg gcctcggtgg acgtctcggg ctggcagctc gagatcggct
     7201 acgaggggaa catcggccac acgacgatgc gcatccccaa gggcacgttt ctcgcgccgg
     7261 gcgggtcgct gctggttgca gtgaacaagt acgactatct ggagagcccg gatgtcgtgg
     7321 cgccggatcc gaccgatcct ccggggctca gcccgctgat ctttcagaac ggtatcggcc
     7381 tggccggagg atccgtc
//
LOCUS       DAOPBQ010000118         7383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9073_length_7383_cov_3.848663, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000118 DAOPBQ010000000
VERSION     DAOPBQ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9073_length_7383_cov_3.848663"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="PKW60_05405"
     CDS             complement(<1..1195)
                     /locus_tag="PKW60_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98901.1"
                     /translation="MNMWICCTLGGWLIATGMSMQGAVALPEPPPSPPQSEWAFVEAL
                     AAPMTWKPHWSRTQALRGEADLGRGVTIEAGFPDPEGLLDTAYQDVRAFFSGTQLPVD
                     GPYRIHTEQIHTSRGETFRLVVQETECRIQAGDTEGIRRGLFFLEDLLLGAEGPFLPL
                     GELERAPFIHTRISRCFFGPIKRPPKNKDELLDDVDYYPDGYLNRIAHDGVNALWLTI
                     EFRNICKTSLTPVVDPDRDRRLEKLRKTVAKCRRYGIQVFVFCIEPRVMDPDNPLLLA
                     HPELGSAPFSGTGRLFCPFSEAAQTYLYEAVYDIFSQVPHLGGLINISYGERSTTCLS
                     AADENWRVACPICAEKAPGEILHASLSAMERGMHAASPSAKLISWLYVPGNGTGVAHS
                     TAPFPD"
     gene            1589..3154
                     /locus_tag="PKW60_05410"
     CDS             1589..3154
                     /locus_tag="PKW60_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPJ98902.1"
                     /translation="MPAGPQLWGLSVFDVLIIIAYIVGVVIIGTYYGKYVKSAKDFFL
                     AGRALPFWAIGMSIVVSDIGASDFVFVGGATYKYGVSAANFDWIGSMPAMAIAAFIFM
                     PYYWRAGVYTIPEFLGRRYNMAVQIIHAAIWGIFMVTMLGVIFQATGNFMASILGWPI
                     WVGVAITIVTVFIYTFSGGLAAVVMTDVVQLVVMFVGGLALLALAMWEVGGWGALQDQ
                     VIAIKGEGHLTMLQPNDDPTTPYPWAGIVFGLGLVMSTAYFSGHQGVVQRVFGARSEW
                     DAKGGMLFGGFLKSFIPLMVALPGLAAIVLLPEGSITKGDDAVPAMIRHLMPAGVRGL
                     TFSALVASFLSSADGYLNSASTIWTTDLYGRVHEFFTGRLPGERHGLVMGRLFTVVFV
                     IIGASLVPVLDRVESMYNFIQTSLSMFQGPVFAILLLGIMWRRANRWGGLIGMLSGVA
                     ISTVLNFTEGLFPSADAFLFVSWWSFVFTIIITVVVSLLTKPEPEEKIRGLVFGQVMK
                     DGQIQRVLAERVE"
     gene            3206..3445
                     /locus_tag="PKW60_05415"
     CDS             3206..3445
                     /locus_tag="PKW60_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98903.1"
                     /translation="MLQPFFDPINNFLGRYYMPWATICALGLFIGAMVWVFTLRKEYV
                     NVDAPRKGFLYDLRVWTIISMLPHVLVYLYFSKWS"
     gene            3448..3672
                     /locus_tag="PKW60_05420"
     CDS             3448..3672
                     /locus_tag="PKW60_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98904.1"
                     /translation="MSDQDPLSHIKRDAEAFFILGAFLIFLAAIVLVATVFQAPGHAR
                     VVNGGAGLMLLLIGGGMAAWSRMLRKRLSK"
     gene            4107..5711
                     /locus_tag="PKW60_05425"
     CDS             4107..5711
                     /locus_tag="PKW60_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPJ98905.1"
                     /translation="MDFTLSAMDITVILGSLLLVVGVGLWASRKQGRTARDYFLASGK
                     LPWWIIGAAFVSTSVSSEQIVGTIGQAYQHGMGIANWEWWLLPVYSVLIIIFIPIYLR
                     NKISTVPELLSRRFNSACGDIYSYIMLFAYIFVFLVPVLYGGSLTFASLTGWNFHYIL
                     WGMIALVAAYTVKGGLMSVVWTDALQCLMLIGGGLLLFFVSLHQVPGGWHAMAEANPE
                     RFHLYCPPDHEVAPFLGVLAGTIGLFVFYSAANQVMVQRVLGARSRWDGIMGIIFAGF
                     INFVRPLVTCFLGFIVYHWIHEMHQAEPLETVDQAFAFSLRYLAPEWGLRGIVLAGFI
                     AAVMSTVSALANSTATIFSLDVYKKLIRPDASERAVVSVGRMTSLSALLIAGAVAPQV
                     ARFGGIFMYFQQGITFIATPIITVILAGIFSRRVNASGALAGLIAGIITTVVLLGLLI
                     AGVKMPIHWLYVGFFQQVLVLAVIVGATLMTPAPAAEVAEPFFWRPAILTEHVRAEPP
                     RPWYARALLWYALFAAIWFYLYWKFW"
     gene            5763..6485
                     /locus_tag="PKW60_05430"
     CDS             5763..6485
                     /locus_tag="PKW60_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPJ98906.1"
                     /translation="MRIMAFGAHPDDIEIFCSGTLAKYAAQGHTIGMAVMTRGDVGSP
                     TLPREEIAAIREREARAAAAIINAEFFWLGYDDEFLYDTPEVRRHVIDVIRQFRPDVI
                     LCPDKDHDYHPDHTRTGQIVWDTHVMATVPNIPTQTPPAGHIHEIWFYDTMAGIDFQP
                     EFYVDVSEHWDTKARMIDCHVSQAEWLIAQYGKTPTHYAGIQTRFRGYQAGCEFAEAF
                     RASRSFPAIATRDILPAKPRVK"
     gene            complement(6507..>7383)
                     /locus_tag="PKW60_05435"
     CDS             complement(6507..>7383)
                     /locus_tag="PKW60_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98907.1"
                     /translation="GFNRRFRMKDGTIRTHPGKMNPGIVAPAGPIDPDVAVIAAEDAE
                     GRLLGCIVNFALHGTTMGGSQLSADWPLYLRETIRGGLGSDIGVVFLNGACGDVTQVD
                     NRSPRTGEFGEGSARRAGMSVGAAALGVLAKMEFTPDAPLGVTSETLALPIRDLGDSD
                     EELVRREAPGIGLGTGDAIYLMEAAMVRAMKEKSPTVPVEMQAMRIGDVGIASNPAEF
                     FCDLGLAVKRSSPWELTMVVELANGCCGYVGTAESYLGGGYEVRTSRCSYLSAGSGEQ
                     MANASARLLCQLAEE"
BASE COUNT         1293 a         2306 c         2171 g         1613 t
ORIGIN      
        1 tgtcgggaaa aggcgccgtg ctgtgcgcga cgcccgttcc gttgcccggc acgtacagcc
       61 acgagatcag cttcgcgctg gggctggcgg cgtgcatgcc tcgctccatc gccgacagcg
      121 aggcgtgcag gatttcgccc ggcgctttct ccgcacagat cgggcaggcg acccgccagt
      181 tctcgtccgc agcgctgagg caggtcgtcg aacgctcccc ataactgata ttgatgaggc
      241 ctcccagatg cgggacctgc gaaaagatgt cgtacacagc ttcgtacaga taggtctgag
      301 ccgcttccga aaaggggcag aacagacgcc ccgtgcctga aaacggcgcg ctccccagtt
      361 cggggtgggc gagcagcagc ggattgtcgg ggtccattac ccggggctca atacagaaaa
      421 caaacacctg aatgccgtat cggcggcatt tcgcgacggt tttgcgcagt ttttccaggc
      481 ggcggtcgcg gtccgggtca actacgggtg tcagtgaggt cttgcagatg ttcctgaact
      541 caatggtgag ccacagcgca ttgacaccgt catgcgcgat gcggttgagg tacccgtccg
      601 gatagtagtc gacgtcgtcg aggagttcat ccttattctt tggcggccgc ttgattggcc
      661 cgaaaaagca cctggaaatg cgcgtgtgta tgaacggggc gcgttcaagt tcgcccagcg
      721 ggagaaaagg cccctccgcg cccaggagca agtcttccag aaagaagagg ccgcgccgga
      781 tcccttccgt gtcgcccgct tgaatccggc attcggtttc ctgaaccaca agccgaaacg
      841 tctcgccccg gctcgtgtgt atctgttccg tatggatgcg atacgggccg tccaccggaa
      901 gctgggtgcc agaaaaaaag gctcgcacat cctgatacgc cgtgtcgagc agaccctccg
      961 gatcaggaaa gccggcttcg atggtgacgc ctcttcccag atctgcttcg ccacgaaggg
     1021 cctgcgtccg gctccaatgc ggcttccacg tcatcggcgc cgccagggcc tccacgaatg
     1081 cccattcact ctgcggagga ctcgggggcg gttcgggcag ggccacggca ccctgcatgc
     1141 tcatgcctgt cgcgataagc catccgccca gcgtgcaaca aatccacata ttcaagaacc
     1201 ctcctcgatg tctgacccgg cagatgggag tggggcacag gatagcaggt acgcggggcg
     1261 atgcgaacat tctcttcaaa cgaacagccc cggccttatc tcaacagcct cagcgcgacg
     1321 aaagcgatcg cggaagacaa catgagtccg cttccgcccg ttttgcgcac ggcgcgcacc
     1381 tggaacgaac acgcttctgg cgtggggcaa cgatgctgtt tcggatactc tgatgatgag
     1441 acaattcgtt cacttgatct gtccgggact ctctgacata ctttgcgagt tgggttcgcc
     1501 gttatcccct tgggccgatg gttcgccggc gcgacacttg aggggtgcga agcggcgaat
     1561 gggcttacat gagcgggaga gcgtgtttat gccagcggga ccccaactct gggggctgtc
     1621 ggttttcgac gtcctcatca tcatcgccta tattgttggc gttgtcatca tcggcacgta
     1681 ttacgggaaa tacgtcaaat cagccaaaga cttcttcctg gcgggccggg cgttgccctt
     1741 ctgggccatc ggcatgtcca tcgtggtgag cgatattggc gccagtgact tcgtgttcgt
     1801 ggggggagcc acgtataagt acggcgtgtc cgccgcgaac ttcgactgga taggttccat
     1861 gcccgcgatg gcgatcgccg ccttcatctt catgccctat tactggcgtg ccggcgtcta
     1921 tacgatcccc gagtttctcg gccgccggta caatatggcg gtccagatca ttcatgccgc
     1981 catctggggc attttcatgg tgacgatgct gggcgttatc ttccaggcca ccggcaactt
     2041 catggcgagc attctaggct ggccgatctg ggtcggcgtc gccatcacca ttgtgaccgt
     2101 gttcatctac accttctccg gcggtctggc ggcagtcgtc atgactgacg tggtccagct
     2161 tgtggtcatg ttcgtcgggg gcctggcgct tctcgcattg gccatgtggg aagtcggcgg
     2221 atggggcgcc ctgcaggacc aggtgatcgc catcaagggc gaggggcatc tgacgatgct
     2281 ccagcccaat gacgacccca ctacgccgta cccctgggcc gggatcgtct tcggactcgg
     2341 gctggtcatg tccaccgcct atttcagcgg gcatcagggc gttgttcagc gggtctttgg
     2401 cgcgcgctcg gaatgggacg caaaaggcgg catgttgttc ggcggattcc tgaagtcgtt
     2461 tattccgctg atggttgcgc ttcccggcct ggccgccatc gtcctccttc ccgagggctc
     2521 gatcaccaag ggcgatgatg ccgtgcccgc catgatccgc catctgatgc cggcgggcgt
     2581 gcgtggcctc accttctcgg cgctcgtagc ttcattttta tccagcgccg acggctacct
     2641 caattccgcc tcgaccatct ggaccaccga cctctatggg cgcgttcatg agtttttcac
     2701 tggacgcctg cccggcgagc ggcatgggct tgtcatgggg cgtctcttca cagtggtttt
     2761 tgtgatcatt ggagcgtcgc tggtgcccgt gctggatcga gtcgagagca tgtacaattt
     2821 catccagacc tcgctgtcga tgtttcaggg gcccgtgttt gccattcttc tcctgggcat
     2881 catgtggcgc cgcgcgaatc gctggggcgg ccttatcggc atgctgagcg gcgtcgcaat
     2941 cagtaccgtg ctcaacttca cggaaggcct gttcccgtcg gctgacgcat ttctcttcgt
     3001 atcatggtgg tcttttgtgt ttactatcat cattacggtt gtcgtaagcc tccttacgaa
     3061 gcctgagccg gaagaaaaga ttcgagggct cgttttcggc caggtgatga aggatggcca
     3121 gattcaacgc gtgctggcgg aaagggttga gtaaatgaac gttcctgaaa ccgccccaaa
     3181 tctgatcacg aggatattca agcccgtgct gcagcccttt ttcgacccta ttaacaactt
     3241 cctgggccgg tactatatgc cgtgggcgac tatctgcgcc ctcggcctgt tcatcggtgc
     3301 gatggtctgg gtgtttaccc tccgcaagga atatgtgaac gtcgacgccc ccaggaaagg
     3361 cttcttatac gatctgcggg tctggaccat catctccatg ctgccccatg tgctggtgta
     3421 tctctacttc tcgaaatgga gctagccgtg agcgaccaag atcctctcag ccatattaaa
     3481 cgtgacgccg aggcgttctt tatcctgggc gcctttctga tctttctggc cgcaattgtc
     3541 cttgtggcaa ccgtcttcca ggcgcccggg cacgcccggg tggtgaatgg cggcgcgggc
     3601 ctgatgttgc tgcttatcgg aggcggcatg gccgcctgga gccgtatgct tcggaagcgc
     3661 ctttccaaat agcctctcac cagcaacggc gccttgattc ccgctccggc tgcccgccgg
     3721 acgccgcgcc tgcgcatccc cgctctacaa cgcctcgcct ggagtgctct cgcgtaccgc
     3781 cctgcaaggc cgcatccggg tggcgattca catgtctgct tcaggagcac cacgaaacgt
     3841 gcgtccgggg cttccgatgt tacataatgg cgccgcgtgc gatttgtggc cagggaagag
     3901 ccgtcatcat gaagtcagcc gcgccgtgct gaaccggagc ccgattcaaa tcccggcggg
     3961 gtgcgcttcc tgcatgctgg aggtttcggc tgcgaacgca cagagtaggg gaaatggggg
     4021 agtatcgcgc gggaaactgg ccggaaatcc cgcagtcttg atgtcaggaa gaggaacggc
     4081 ctctgaacac atctggagca ggaccgatgg attttacact gagcgcgatg gacatcacgg
     4141 ttatcctggg ctcgctattg cttgtcgtcg gcgtaggact ctgggcctcc cgaaaacagg
     4201 gccgcacggc acgcgattac ttcctcgctt ccggaaagtt gccttggtgg atcatcgggg
     4261 ccgcgtttgt atcgaccagc gtgtcgagcg aacaaatcgt cgggacgatt ggccaggcat
     4321 accagcacgg aatgggcatc gccaattggg aatggtggct gctccccgta tacagcgtgc
     4381 tcatcatcat ttttattccg atctatctcc gcaacaagat ctcgacagtc ccggagctgc
     4441 tttccaggcg cttcaactcg gcctgcggcg atatctacag ctacatcatg ctgttcgcct
     4501 acatcttcgt ctttcttgtg cccgtgctct acggcggcag tctcaccttt gccagtctga
     4561 ccggatggaa cttccactac attctctggg gcatgatcgc actggtggcc gcatacaccg
     4621 tcaagggcgg cctgatgtcc gtcgtgtgga ctgacgccct gcagtgtctc atgctcattg
     4681 ggggcgggct gctcctcttt tttgtatcgc ttcatcaggt gcccgggggc tggcatgcca
     4741 tggccgaagc caatcccgag cgctttcatc tgtactgccc tcccgaccac gaagtcgcgc
     4801 cgtttctagg cgtattggcc gggaccatcg gcttgtttgt gttctattcc gccgccaacc
     4861 aggtgatggt gcagcgcgtt cttggagcac gttcgcggtg ggacggcatc atgggcatca
     4921 tttttgccgg gttcatcaat tttgtccgtc ccctggtcac ctgctttctc ggattcattg
     4981 tgtaccactg gattcatgag atgcaccagg ccgaaccgct cgaaaccgtc gatcaagcct
     5041 ttgccttctc cctgcgctac ctcgccccgg agtggggact gcgaggcatc gtcctcgccg
     5101 ggtttatcgc cgccgttatg tccacggtca gtgccctggc caactccacc gcaacgatct
     5161 tctccctcga tgtttacaaa aagctcatcc ggcctgacgc cagcgagcgt gcggtggtct
     5221 cggttggacg gatgacctct ttgtcggccc tcctgatcgc cggcgccgtg gcgcctcagg
     5281 tcgcgcgttt cggcggcatc ttcatgtatt ttcagcaggg cattaccttt atcgccactc
     5341 ccatcatcac ggtcatcctc gccggaatct tctctcgacg ggtcaacgct tccggcgcgc
     5401 tggccggcct gattgcgggt attatcacca cggtcgtgct cctcggcctt ctcatcgccg
     5461 gtgtgaagat gccgatacac tggctctatg tggggttctt ccagcaagtg ctcgtgctgg
     5521 ccgtcatcgt gggggcaacc ctcatgacac ccgccccggc tgcggaagtc gcggagccgt
     5581 tcttctggcg acctgccatc ctgaccgaac atgtgcgagc cgaaccgcca agaccctggt
     5641 atgcgcgggc gctgctttgg tacgctcttt ttgccgcgat ctggttctat ctgtactgga
     5701 aattctggta agcgccgcac gcggacagcc gcgcgggctt caacctcagg gagaaatgag
     5761 cgatgcgcat tatggctttc ggggcacatc ctgacgatat cgagatcttc tgctccggca
     5821 ctctcgcgaa atacgccgcg caggggcata ccatcggcat ggccgtcatg acccggggcg
     5881 acgtcggctc acctaccctg ccccgcgaag aaatcgccgc tatccgggag cgtgaggcgc
     5941 gtgctgccgc cgccatcatc aacgcggagt tcttctggct ggggtacgac gatgaatttc
     6001 tctacgatac gcccgaagtg cggcggcatg tcatcgacgt gatccgccag ttccgccccg
     6061 acgtcatcct ctgcccggat aaagaccatg actaccaccc cgaccacacc cgcaccgggc
     6121 agattgtctg ggacacccac gtgatggcca ccgtccccaa tatcccgacc cagaccccgc
     6181 cggccggcca tattcatgag atctggttct acgacaccat ggccggcatc gattttcagc
     6241 ccgagttcta cgtcgacgtc tccgaacact gggacaccaa ggcgcgtatg atcgactgtc
     6301 atgtcagcca ggcggagtgg ctcatcgccc agtatgggaa gaccccgaca cattacgctg
     6361 gaattcagac ccggtttcgg gggtaccagg ccgggtgcga gttcgctgag gcattccgcg
     6421 catcgcgatc cttcccggcc atcgcaacac gagacatcct gccagcgaaa ccgcgcgtca
     6481 aatgaggcgc ctcaggtgac gggcgctcac tcctctgcaa gctggcagag gagacgcgcc
     6541 gacgcattgg ccatttgttc tccgctgccc gcggagaggt agctgcagcg ggaggtgcgg
     6601 acttcgtagc ctccgccgag gtacgattcg gcggtgccca catatccgca gcagccgttg
     6661 gccagttcga cgaccatcgt gagctcccag ggggagctgc gttttacggc aagcccgaga
     6721 tcacagaaaa actccgccgg attgctcgcg atgcctacgt cgccgatgcg catggcctgc
     6781 atttccacgg gcacggtcgg cgatttctct ttcatcgccc gtaccatggc cgcttccatc
     6841 agatagatgg catcgcccgt gcccagcccg atgcccggcg cttcccggcg cacgagttcc
     6901 tcgtccgagt cgccaagatc gcgaatgggg agggccagtg tctctgatgt cacgccgagt
     6961 ggcgcgtccg gtgtgaattc catcttggca agcacgccca gggcagccgc gcccacgctc
     7021 atgccggccc ggcgcgcaga cccttccccg aactctcccg tgcgcggact gcggttgtct
     7081 acctgcgtca cgtcgccgca ggctccgttc aaaaacacca cgccgatatc actgcccagt
     7141 ccgccccgga tggtttcccg cagataaagc ggccagtcgg cggagagctg gcttccgccc
     7201 atcgtggtcc cgtgcagcgc gaaattcacg atacatccca gaagccggcc ttcggcatct
     7261 tccgctgcaa ttaccgccac gtccgggtcg atcggtccgg ccggcgccac aatgccagga
     7321 ttcatcttgc cggggtgggt ccgtatcgtg ccgtctttca tgcgaaaccg gcgattgaac
     7381 ccc
//
LOCUS       DAOPBQ010000119         7358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9113_length_7358_cov_4.216897, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000119 DAOPBQ010000000
VERSION     DAOPBQ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9113_length_7358_cov_4.216897"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PKW60_05440"
     CDS             complement(<1..922)
                     /locus_tag="PKW60_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98908.1"
                     /translation="MKPLLFTMITLSVAAVLGGCATIHGAHTEANIAQTREPDPCMGD
                     WEGKWGSANLCAQVIPLSDKTYQMNLLPAFDTREPALAVLEGKAVDDGVDFSGTGVNG
                     DMKDAKITITFRRGRMNGRVEGPVSGAIDLHHVVRLSPTLGARPPRSAIVLFDGDNLD
                     LWEARSVDPWGINLKKIVGGENCAAYLRTWIFAPAPGKAQFACGIEDGGKVWLNGNEI
                     HAALEERRVTPCYYKILVDLQEGWNTFLVKVVQNDKDWAFTACLRGLDASPASGFFAG
                     TPGLDVYPLESFDGNMSLWEVCGPYAADGKS"
     gene            1154..2584
                     /locus_tag="PKW60_05445"
     CDS             1154..2584
                     /locus_tag="PKW60_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPJ98909.1"
                     /translation="MTDTMELRYQERLDRYVTALRNKQPDRVPIRPFVAEFAGKYAGY
                     TCQEVTHDYRKAFDAVCRCALDFDWDAMVGNMVYVWTGLTQAIGLRYYGTPGIDVPAD
                     TGFQYLEPLQGEEFMRADEYDALIADPTAFLFDVWLPRVSRDIVPPGAPSTFRSNLAF
                     LKGGMAMWDYFGGFGAQAGRMRAECGMPGAIAGILKAPLDILGDKLRGYVGLSMDLFE
                     QPDKVKAACEALAPHLLSTALATADPAKDVPITIWMHRGCVPFISMDQFKALYWPTLK
                     PIIEAIWANGHQVLFYAEGDWNAHLESFAELPEGSIIYHVDKADIFEAHKVLGNRFCI
                     SGGIPNFLLTYGTVDEVKDRCKKVIDEVGADGGFVMDASAIIQNDAKIENMRAMTEFT
                     REYGVYRGGVSEPAENAEWKKAAAPETPPNVGRSVNRPPGVCIPWEEKKAELPPIQGD
                     AGIVKRIWEEIDTFGFTFIMHCLVSF"
     gene            2825..6388
                     /locus_tag="PKW60_05450"
     CDS             2825..6388
                     /locus_tag="PKW60_05450"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPJ98910.1"
                     /translation="MTIQKKTLIIIAVTLVCLLAGLYLGVRFILLRGFDDYEMDRARS
                     ETALIRRSYLARLNELDVHLQQLSAWDAMASYVQHPTPEFEESNFVNNAFESLELNAV
                     VVLDRTGRVVFARGYDLLERRTQPISPGLMEVIEAIPELQDHDTARSARAGLISLPED
                     PMLVASRPIVSSNFDGPIRGTLILGRYADGTLATRCGDLLKRSVTALRWQDVPPEVQA
                     GLADAAGIYVRPMSRRLLEAYARVNDIIERPALALRTELPREAYAAKVILARTLMASL
                     LAAGLVFGTVVMLLLRYSVLLRVASLESEVRGIADSGDARRRVALSGRDELARLAESV
                     NVMLAALAASREALRSSEETSRALMDATLDSAFLATRDGTLVAVNEAGARRLGMNREE
                     ITGAKLRDLFLPALAEARMARLEEAFETGRAVRFEDRRGDIRFDVTYYPVANAEGRID
                     RLAIFAHDITDMRKAEEELRESRARYRDLVQSVNSIVLRWDLNGVVTFVNEYGQQFFG
                     WTWQELVGKPVVGTIVPEVDSSGQDLRGFIDALLREPEKYVSNENENIRKNGERVWIA
                     WSNRPVFDDTGALVEILSIGNDITRRRLVEQSLVHRVQMEELAASVSARFLTAGTGDL
                     SGTMQEVLRSVGEFAGADRAYFYLAREDGKTLDLVHEWCAPGVVSPDRPETGLSAERY
                     TWFAEKLRNDGVVYIRDAAELPEEAAAERALLDSWGMRSLLDVPLLWRGQVVGILGFS
                     GISQKADWDNDDIRLLKLVAGVFVAAFQRARAEEAVLVQARRLEALINLQDMGQSGPR
                     EMMGFVLQKAVDLTRSDLGFVSMLNGRDEAFVPDNWARADEVDDALPVETVAAALETP
                     EMWDRLKDARKPLVLDRASLPGQAAKYITRLLAVPILDHDRLEAMAVVVNKPFEYDEA
                     DANQLQLLMSGAWNQIKRNEAVAWIQREVDEIANIQRALLPRSMPTVQGMRVRAFSST
                     FDRAGGDYYDVLPVGAPPGTDLKQHPRWLILIADVSGHGPSSAVIVAILSTLLRVGSV
                     EGSSPARVLEYLNGQLMGHTAGQVFVTAFLALIDLDARTLVYSNAGHNPPVVRRPDGT
                     VTELERSGDIPLGIMTSWTYSERTIPIERDAVLWLYTDGVVETRSADGEAFGEQRLRD
                     AIQSLEGESSRAVAELVGMLRAHEAGRRPSDDQVIMLVEFT"
     gene            6427..>7358
                     /locus_tag="PKW60_05455"
     CDS             6427..>7358
                     /locus_tag="PKW60_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HPJ98911.1"
                     /translation="MHGVRYTVSVVWAASWVLIMPHVAAQESQPLSAAQNGEQVDVLV
                     DGALFTSYKFSPEQKYPYFWPVIGPVSGKTVTTETSEPYPHHHSLFFGCDRVNGGNYW
                     QEGNERGQILSQGPKIVEAEGKRVVFEDECLWKLPGQEPVLRDTRTITVTAPNRATRL
                     IDFTIVLHPLVQIQIEKTNHSLFSARMVPELSVASGGTLINAEGARAETGTFGVASPW
                     CDYYGTRDGVTEGLAILQHPLNPWYPAKWFTRDYGFFSPTPMFWPSGGRTVLPAHEPV
                     TLAYRVVVHAGDAKEAGITDLFEAFQAATPLQNR"
BASE COUNT         1369 a         2169 c         2443 g         1377 t
ORIGIN      
        1 cggatttccc gtccgcggcg taggggccgc agacctccca gagcgacata ttgccatcaa
       61 acgactcgag aggatagacg tccagcccgg gcgtaccggc aaagaatccg gaggcgggcg
      121 aggcatccag acctcgtaaa caggcggtga aggcccagtc cttgtcattc tggaccactt
      181 tgaccaggaa ggtgttccat ccctcctgga ggtcgacgag gattttgtaa tagcaggggg
      241 tgacccttcg ctcttcgagg gcggcgtgga tttcgttgcc gttgagccag accttgccgc
      301 catcttcgat gccgcaggcg aattgtgcct tgccgggagc aggcgcaaag atccaggtcc
      361 gaaggtaggc ggcacagttt tcaccgccca cgattttctt gaggttgatg ccccaggggt
      421 cgacgctgcg tgcctcccag aggtcgaggt tgtcgccgtc gaacagcacg atggcgctcc
      481 tgggcgggcg ggcgccgagc gtgggggaga ggcgcaccac atgatggagg tcaatggcgc
      541 cggagaccgg gccttcaacg cggccgttca tacgtcctct ccgaaacgtg atggtgattt
      601 tggcgtcttt catgtcgccg ttaacgccgg tgccggaaaa atcgacgccg tcatcgaccg
      661 ccttgccttc gagcactgcc agggcgggtt cccgggtgtc gaatgccggg agcaggttca
      721 tctgataggt tttgtcggac agaggaatga cctgcgcgca taaattggca ctgccccact
      781 tgccttccca atcgcccatg cagggatccg gctcgcgggt ctgggcaatg ttggcttccg
      841 tgtgggcgcc gtgaatcgtg gcgcaaccgc ccagaacggc ggcgacagac agtgtgatca
      901 tcgtaaacaa gagcggtttc atgattcggg catctccttg taccgcgata gacgctcggc
      961 gtgggcacag gcgtcaaaaa cagagagtag ggttttggag gacatgattt caaggcctcc
     1021 tttgatttcg atgaacagac tgccgggaag tggacaggcg ttgtcttgaa tgcctactat
     1081 ggagtcaggg acagcgtgtg agggaggatg ggatgcgccg cgcgccggtg tacacagtcc
     1141 agggagacta gagatgacgg ataccatgga actgcgttat caggaacggc tggaccgcta
     1201 tgtgacggcg ctgcgcaaca aacaacccga tcgggtgccg atccgtccct ttgtagctga
     1261 atttgcgggg aaatacgcgg gctacacgtg ccaggaggtg acgcatgatt accgcaaggc
     1321 cttcgacgcg gtctgccggt gcgcgctcga tttcgattgg gacgccatgg tcggcaacat
     1381 ggtgtatgtc tggaccgggc tgactcaggc catcgggctg cgttattacg gcacgccggg
     1441 tattgatgtg cccgcagaca ccggattcca gtatctcgag ccgctccagg gcgaggaatt
     1501 catgcgtgcc gatgaatacg acgcgcttat cgccgatccg accgcgtttc tattcgatgt
     1561 ttggctgcct cgcgtgtcga gagacattgt cccgccagga gcgccatcga cgttccggag
     1621 caatttggcg tttctgaagg gcggcatggc catgtgggac tattttggcg gatttggcgc
     1681 gcaggcggga cgtatgcgcg ctgaatgcgg catgcccggg gccattgcag gaatcctcaa
     1741 ggcgcctctg gacatcctgg gcgacaagct gcgcggctac gtcggactca gcatggactt
     1801 gttcgagcag ccggataaag tgaaggcagc ctgtgaggcg ctcgcaccgc atctccttag
     1861 cacggccctc gcgaccgccg acccggcgaa ggatgtgccc atcaccatct ggatgcaccg
     1921 cggctgcgtg ccgtttatct ccatggacca gttcaaggcg ctgtactggc ccacgctgaa
     1981 acccattatc gaggccatat gggccaacgg ccaccaggtc ctgttttacg ccgagggcga
     2041 ctggaatgcc cacctcgaat cgtttgccga gcttccggag ggaagcatca tctaccacgt
     2101 tgacaaggcg gatatcttcg aagcgcacaa agtcctcggg aaccggttct gcatcagcgg
     2161 cgggatcccg aatttcctct tgacctatgg caccgtggat gaggtcaaag accgctgcaa
     2221 gaaggtgatc gacgaggtgg gcgcggacgg tggttttgtc atggatgcga gcgcgattat
     2281 ccaaaacgac gccaaaatcg aaaacatgcg cgccatgacc gaattcaccc gcgaatacgg
     2341 tgtgtatcgc ggcggcgttt ccgagcctgc tgaaaacgcc gaatggaaga aggccgccgc
     2401 acccgagacc ccgccaaacg tgggccgttc cgtcaatcgc ccgccgggag tatgcatccc
     2461 ctgggaagag aaaaaggctg aattgccgcc cattcaggga gatgccggca tcgtaaagcg
     2521 gatctgggaa gaaatcgata catttgggtt cacgtttatc atgcactgcc tggtctcgtt
     2581 ttgacgcggc ggtattgcgg gcgcagcggg cggacgtgtc ccgggttgcc gggcgggggc
     2641 gcgcggcaat gaaccatctg aatgcggtgt taccccggag caggcgctgc gctccgcggc
     2701 gtttacttgt cctccgcggc gttgctccat gccccgaggc cggaaagcta ccgcttgccg
     2761 cgaccgtttt gtcatactgc cgccgggcgg atacggggaa tccgggggcg ggtgaattgc
     2821 cgttatgacc atacagaaga aaacactcat tatcatcgcg gtaaccctgg tttgcctgtt
     2881 ggcggggctg tacctgggcg tccggttcat tctgttgcgc ggattcgacg attatgaaat
     2941 ggaccgggcg cggtccgaaa ccgccttgat ccggcgctcc tacctggcgc gcctgaacga
     3001 actcgatgtg catttgcagc aattgtcggc atgggacgcc atggcgtcct atgtgcagca
     3061 tcccaccccg gagtttgaag agtccaactt cgtcaacaat gccttcgaat cgctggaact
     3121 gaacgcggtg gtcgtcctgg atcggacggg gcgggtggtg ttcgcgcgcg gatacgacct
     3181 ccttgagcgg cgaacccaac ccatttcgcc agggctcatg gaggtgatcg aggcgatccc
     3241 ggagctgcag gaccacgata cggcccgctc agcgcgggca ggcctgatct ccctgccgga
     3301 agaccccatg ctggtggcat cgcgtccgat cgtgtcgagc aatttcgacg gacccatccg
     3361 ggggacgctc attctgggcc gttacgcgga cgggaccctg gccacgcgct gcggcgacct
     3421 gctcaaacgc tccgtcacgg cgctgcggtg gcaggatgtg cccccggagg tccaggcggg
     3481 actggcggat gccgcgggga tctatgtgcg cccgatgagc cgcaggctgc tggaggcgta
     3541 cgcacgggtc aatgacatta tcgagcgccc ggcgctggcg ttgcgcacgg agttgccgcg
     3601 agaggcgtac gcggccaaag tcatcctggc gcggaccttg atggcgtcgc tgctcgctgc
     3661 cggtctggtg ttcggcacgg tggtcatgct cctcctccgg tattcggtgc ttttgcgcgt
     3721 ggcgtcgctc gagagcgaag tgcggggcat agcggattcg ggcgatgcgc gccggcgggt
     3781 ggcgctcagc gggcgcgatg aactggcgcg gctggcggaa tcggtcaacg tgatgctcgc
     3841 agccctggcg gcgtcccgcg aggcgttgcg ctccagcgaa gagacctcgc gggccttgat
     3901 ggacgccacg ctggattcgg cgtttctggc gacccgcgac ggcaccctcg tggccgtaaa
     3961 cgaagccggc gcccgccgcc tgggcatgaa ccgtgaggag atcaccggag cgaagctgcg
     4021 cgaccttttt ctgcccgccc tggcggaggc gcgcatggcc cggctggagg aagcgttcga
     4081 aaccgggcgg gccgtgcgtt tcgaggaccg gcgcggcgac atccgtttcg acgtgaccta
     4141 ttatcccgtc gcgaatgcgg aagggcggat cgaccgcctc gccatctttg cccacgacat
     4201 cacggacatg aggaaagccg aggaggagct tcgcgaaagc cgggcgcgtt accgggacct
     4261 cgtgcagagc gtcaacagta tcgtgctgcg ctgggatctg aacggcgtcg tcacgttcgt
     4321 gaatgaatat gggcagcagt tcttcggatg gacctggcag gagctcgtgg gcaagcccgt
     4381 cgtggggacc attgtgccgg aggtcgacag ctccggacag gatcttcggg gcttcatcga
     4441 cgcgctcttg cgggagccgg aaaagtatgt gtctaacgag aacgaaaaca tccgcaagaa
     4501 cggagagcgg gtgtggatcg cctggagcaa ccgccccgtt tttgatgaca caggcgccct
     4561 ggtcgagatc ctttcgattg gcaatgacat cacccgccgg cggctcgtgg agcaatcgct
     4621 cgtgcaccgc gtgcaaatgg aagagctggc cgcgtcggta tccgcccgtt tcctgaccgc
     4681 gggaaccggc gacctctccg gcacgatgca ggaagtgctg cgcagtgtag gtgaattcgc
     4741 cggcgccgac cgggcgtatt tctacctggc gcgggaagac ggcaagacgc tcgacctcgt
     4801 gcacgagtgg tgcgcgcccg gcgtcgtctc tccggaccgg cccgaaaccg ggttgtcggc
     4861 ggaacgctac acctggttcg ccgaaaaatt gcggaacgat ggggtggtgt acatccgtga
     4921 cgctgccgag ttgccggaag aagcggcggc ggagcgggcc ctcctcgatt cctgggggat
     4981 gcgcagcctg ttggacgtgc ccctcctctg gcgtggccag gtggtgggca tcctgggatt
     5041 cagcggcata tcgcagaagg cagactggga caatgacgac atccgcctgc tgaagctggt
     5101 ggcgggagtc tttgtcgcgg cgtttcaacg ggcccgcgcc gaagaagccg tgctcgtgca
     5161 ggcgcgccgg cttgaggcgt tgatcaacct gcaggacatg ggtcaatccg ggccgagaga
     5221 gatgatgggc tttgtcctgc agaaggccgt cgacctgacc cggagcgatc tggggtttgt
     5281 cagcatgctc aacgggcgcg atgaagcgtt tgtgccggac aactgggcgc gcgcagacga
     5341 ggtggacgat gcgctgccgg tcgagaccgt ggcggccgcc ctggaaactc cggagatgtg
     5401 ggacaggctc aaggatgcgc gcaaacccct ggtgctggac cgtgcttcgc tgcccggcca
     5461 ggccgcgaag tacatcacgc ggctgctcgc ggtgcccatc ctcgaccacg accggctcga
     5521 agccatggcc gtggtcgtga acaaaccctt cgaatacgac gaagccgacg ccaaccagct
     5581 gcaactgctc atgagcggcg catggaacca gatcaaacgg aacgaggcag tggcctggat
     5641 tcagcgcgaa gtcgacgaga tcgccaacat ccagcgggca ctgctgccgc gctcgatgcc
     5701 cacggtgcag gggatgcgcg tccgggcctt ttcatcgacc tttgaccgcg ccggaggcga
     5761 ttactacgac gtgctaccgg tgggggcgcc gccgggcacc gacctgaaac agcatccgcg
     5821 ctggctgatt ctgatcgccg atgtctcggg gcatggcccg tcgtcggcag tcatcgtggc
     5881 catcctgtcg accctgttgc gggtgggatc ggtcgagggg tcgtcgcccg cccgggtact
     5941 ggagtatctg aacgggcaat tgatgggcca cacggctggc caggttttcg tgaccgcgtt
     6001 tctggctctg atcgatctgg acgcacgcac cctggtctat tccaacgcgg gacacaaccc
     6061 ccccgttgtg cggcggccgg atggcaccgt gactgaactc gaacgctcgg gtgacatccc
     6121 ccttggcata atgaccagtt ggacctattc ggaacggacc atccctatcg agcgggacgc
     6181 ggtcctgtgg ctgtatacgg acggagtggt cgaaacgcga tccgctgacg gcgaggcatt
     6241 cggcgagcag cgcctgcgcg atgccattca atcattggaa ggggagtcgt cgagggcggt
     6301 tgccgagctg gtcgggatgc ttcgcgccca cgaagcggga cggcgcccca gcgacgacca
     6361 ggtcatcatg ctggtcgagt ttacgtagtt ccggaaacgc ggtctgaacg gagcggagat
     6421 tacgcgatgc acggcgttcg atacaccgtc agtgtggtct gggcggcatc ctgggtgctc
     6481 atcatgcccc atgtggccgc gcaggagagc cagccgcttt ccgcggcaca gaacggcgaa
     6541 caggtggatg tcctggtcga tggcgcgctc ttcacaagct acaaattctc gccggaacag
     6601 aaatacccct acttttggcc ggtcatcgga ccggtgtccg gcaaaaccgt caccacggag
     6661 acctccgaac cgtatccgca ccatcattcg ctgttttttg gatgcgaccg ggtgaacgga
     6721 ggcaattact ggcaggaagg caacgagcgc gggcagatcc tctcacaagg gccgaaaatt
     6781 gtcgaagctg agggcaaacg ggtcgttttc gaagacgagt gcctgtggaa actgccgggc
     6841 caggagcccg tgctgcgcga cacgcgcacg atcacggtga ccgccccgaa tcgggcgacg
     6901 cgcctgattg atttcacgat cgtccttcat cccctcgtcc aaatacagat tgaaaaaaca
     6961 aaccattctc tgttttcggc gcgaatggtc cccgaactca gcgtggcgtc gggcggcacg
     7021 ctgatcaacg ccgaaggagc ccgggccgaa acgggcacgt tcggggtggc ctcgccgtgg
     7081 tgcgattact acggcacgcg cgatggtgta accgaagggc tcgcgatcct ccagcatccg
     7141 ctcaatccat ggtatccggc aaaatggttc acgcgcgact acggtttctt ctcgccgacg
     7201 cccatgttct ggccgtccgg cggccgcacg gtgctgccgg cgcacgagcc cgttacgctg
     7261 gcataccggg tcgtcgttca tgctggtgac gcgaaagaag ccgggatcac ggacctcttt
     7321 gaggcatttc aggccgccac acctctccag aatcgcta
//
LOCUS       DAOPBQ010000120         7352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9119_length_7352_cov_4.485268, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000120 DAOPBQ010000000
VERSION     DAOPBQ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9119_length_7352_cov_4.485268"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1489
                     /locus_tag="PKW60_05460"
     CDS             191..1489
                     /locus_tag="PKW60_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98912.1"
                     /translation="MPAFMLPFAVLLSALTAATPTVIVDIDVNDEVHGRPEPMTEADV
                     DALVGSLHESGAEVLLVRMGFLGVLPFRTSLSYPLEFDEDHARLHQCAAVGDIEAYIA
                     VRKEAMARYRSFIESCNPPEAFIAAAHKRGMKALIWIDIYDNFYPGYRSKFLEEHPHC
                     VWTARDGTLRFPGLISYAFPEARAFCLAQAKELLALGADGIHCSTSAHCRHMPNSHKT
                     DFYGYEQPVVEAFQARYGVNIRTAGDFDKEAWHDIKGEFVVQLYRELAELCHAQGKEL
                     WLGLQLGRYTQFTVDPHFSTHNVARYSNHWKTLVDEGIVDAFVLGDYEIASNPGHACW
                     TVKPDIQLAEGQDLFQWAAAHYREYCAGRTELYLFSEWLPAGPEALESRMNDWASRVL
                     ENGFDGIDVHEAWNFESPPESMAVLKQFAERLAGQSPSRE"
     gene            1656..2159
                     /locus_tag="PKW60_05465"
     CDS             1656..2159
                     /locus_tag="PKW60_05465"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HPJ98913.1"
                     /translation="MNSRRIATSGLLVLAVVALAYYATREMAAKAPPAANTGVSVSPE
                     EPDTEASPSGAGRQVFVYYFHRTMRCDTCRAIETGSFHALSGAFEKELASGALVWTAI
                     NLDEPENGHFIEDFNLSSNGVVVAVVKDGAVTEWKNLDQVWELYTDEAALGRYVTEEV
                     AARLKRD"
     gene            2414..3325
                     /locus_tag="PKW60_05470"
     CDS             2414..3325
                     /locus_tag="PKW60_05470"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HPJ98914.1"
                     /translation="MAYALEASEAAAGGLKRVALEQLDQALASARNPGTQRDEAVHDV
                     RKCMKKLRAILRMAREELGEGVFKRENACFRDAAQRLSGLREAAVRMATAEKLQTHFG
                     GQDSGLFIPLISNLQALAEQARREHLDESDALCEVAAMLDGARARVDGWPLSGRGFSA
                     VANGVRRIYQQGRKGLRLARKKATDEQFHEWRKAVKYLWYHGRLLQSIWPAMMSVLVE
                     ELDVLGDTLGQDHDLADLRVALIQTPALAGAPPAFETVLHLGAQRQDELRRAALSLGA
                     RLYAEKPRAFVDRLGVYWKVSHAPPAG"
     gene            complement(3389..5536)
                     /gene="glgP"
                     /locus_tag="PKW60_05475"
     CDS             complement(3389..5536)
                     /gene="glgP"
                     /locus_tag="PKW60_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPJ98915.1"
                     /translation="MEISQRFRELARNLYWTWHPRVIAIFRDIDPELWREVNHNPVAF
                     LKQVPAETLRKRGTELAIDTRITHAFHQLNGYLAEQSTWGSRTGGPTRLNPVAYFSAE
                     FGLHESLPIYSGGLGVLAGDHLQAASDLGVPLVGIGLYYAYGYFNQRLDAEGWQREEY
                     LEAEVEDLPIDVATDSQGRPLRVLVENGSDQTWARVWRAEVGRCSLILLDTNVEENSE
                     EDRGLTGQLYGGDARTRLRQELILGIGGMRALEALGIHPSVIHLNEGHSAFATLEAAR
                     QVLERENRSFEDVRERVSNRTVFTTHTPVEAGHDRFPADMLLGMLEPLRAQLRISGNE
                     LLALGRVDPHDDGETFCMTVLGLKMARYRNAVSAIHERVSRRMWRGLWPSRPVEEVPI
                     YHITNGVHTPTWLAMSMVRLYSRYHGEDWADRAHLAQTWAHVEEIDYVEFWEQHQILK
                     TSLVDYVRRCVAHQIAQRGGPGVSCLSPEDCLKHDVLTVGFARRFATYKRATLLFGDL
                     PRLDRLVNHKERPVQFILAGKAHPNDSNGKSLIKQVYDVTQMPEFRGKIVFIENHNIN
                     VSRHLVQGIDVWLNNPRRPQEACGTSGQKVVLNGGLNLSVLDGWWAEAYDGHNGFAIG
                     AGEEHSDPEQQDASDREALFACLENEVAPLYYARDSDGIPRDWVAMQKHALRTLPWRF
                     SASRMVMDYVHSCYLPAAGTLTSTYPGINSHRA"
     gene            5989..6918
                     /locus_tag="PKW60_05480"
     CDS             5989..6918
                     /locus_tag="PKW60_05480"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HPJ98916.1"
                     /translation="MKTAACLLLCMVAGSPLLLHAANADTPPGPASDELAKMREAAPG
                     KATAAPAQPRKVLVCSLVTADYVHSAIPYGEAALQIIAEKTQAFSVECSNDIAMFEPE
                     NLNRFDAVFFNNAYGELFLPAGFDALPDAQKEALRARAATLRQSLADFVRNGKGIVAL
                     HAGLAIFEEWEEWGEIIGGRFDNHPWNKVVTIRVEEPAHPLLQAFKEPEFQVHDEIYQ
                     FTGPYSRNALRVLLSLDLEKTGEPNAAEVHRTDGDFALAWIKPYGKGRVFYCGLGHEH
                     DLFWNPAILRFLLDGTQFAAGDLQADMTPSANR"
BASE COUNT         1414 a         2242 c         2228 g         1468 t
ORIGIN      
        1 ccgttacgtc gggaaggaaa cgaaacgaca ccgccccccg tccgtcgttg gtgaagatct
       61 cggcagagga gcgtatgcca gcgggcaggt cttgtttttg tcggttgcgt tctgtagaac
      121 ggacagcgcc cgggatgagg ccctgtggtg aggcgtgaat tcatcatctc cgggtagaag
      181 gagaggtcca atgcccgcgt ttatgttgcc gtttgccgtc ctgctctctg cgttgactgc
      241 cgccactccg accgtgattg tggatattga cgtcaacgat gaggtccacg gacgtccgga
      301 accaatgacc gaagccgacg tggacgcgct cgtgggcagc ctgcacgaaa gcggcgccga
      361 ggtgctgctt gtgcgcatgg gatttctggg ggtgttgcct ttccgtacct cgctgagcta
      421 tcccctggaa ttcgacgaag atcatgcccg gcttcatcaa tgcgccgcgg tgggcgatat
      481 agaagcgtac atcgccgtcc gtaaagaggc tatggcgcga taccgttcct ttatcgaatc
      541 gtgcaatccg ccggaggcct ttatcgcggc cgcccacaaa cgcgggatga aagctctgat
      601 ctggatcgat atctacgaca acttctatcc gggctaccgg tccaagtttc tggaagagca
      661 cccccactgc gtctggaccg cgcgcgacgg caccctgcgg ttcccggggc tgatcagcta
      721 tgcgtttccg gaagcgcgcg cgttctgttt ggctcaggca aaagaattgc tcgccctggg
      781 cgccgacggc attcactgct cgaccagcgc ccattgccgc cacatgccca attcgcacaa
      841 gaccgacttc tacggctacg agcaacccgt tgtggaggcg tttcaggcgc gctacggcgt
      901 caatatccgg actgccggcg atttcgataa agaagcgtgg cacgacatca aaggcgagtt
      961 cgtcgtccag ctctaccggg aactcgccga gctttgccac gcccagggca aagaactctg
     1021 gctcggactt cagctcggac ggtataccca gttcacggtg gacccccatt tcagtacaca
     1081 caacgtggcg cgctacagca atcactggaa gacgctggtc gatgagggta tcgtagacgc
     1141 gtttgtgctt ggcgactacg agatcgcgtc caatcccggc catgcctgct ggacggtgaa
     1201 gcccgacatc cagctcgccg aaggacagga cctctttcaa tgggctgccg cacactaccg
     1261 ggaatactgt gccggcagga cagaactcta ccttttctcg gaatggctcc cggccggccc
     1321 cgaggcactc gagtcgcgca tgaacgactg ggcgtcgcgc gtacttgaaa acggattcga
     1381 cgggattgac gtacacgaag cctggaattt cgaaagcccg cccgaaagta tggccgtgtt
     1441 gaaacagttt gccgagcggc ttgccgggca aagcccgtca cgtgaatgag gaatgcgcta
     1501 gtagccattc tgtgcgatcg ggcccgcgcc gaaagttggc gattcccggc tgccgcgggc
     1561 ataattccag agccgccgag tgccgaactc gcgggaatgg cgtcactata agccctggtt
     1621 tttcgtaata gccgcgcacg ttggcaggag gttccatgaa ttcacgaagg atcgcaacaa
     1681 gtgggctgct tgtgttggcc gtggtggccc tggcctatta tgcaacccgt gagatggcgg
     1741 ccaaggcgcc gccagcggcg aataccggcg tgtccgtatc cccggaggag ccggataccg
     1801 aagcctcccc ctccggggcc ggccgccagg tgtttgtcta ctactttcat cggacgatgc
     1861 gatgcgacac ctgccgtgcc atcgaaacgg gttcgtttca cgcgctgtcc ggcgcattcg
     1921 agaaggagct ggcgagcgga gcgctggtct ggacggccat caacctcgac gaaccggaga
     1981 atgggcattt cattgaagat ttcaacttgt ccagcaacgg cgttgtggtg gcggtcgtca
     2041 aggacggcgc cgtaaccgaa tggaagaatc tcgaccaggt gtgggagctc tatacagacg
     2101 aggcagctct gggccgctac gtgaccgaag aggtcgctgc caggctgaag cgagattgac
     2161 gccagaaaac gcgttcgcgg tggtatgcgc ccttttggcc ggggatcctc atctcactca
     2221 atccccctct tgcggtgctc cttgtggcgg gatttggcgt agcacggaaa tgcctgacca
     2281 gctgtccgcg cgtgtagccg ttggattgtg cgcgtcatag atgccagaat ggacgccagc
     2341 gcggtgtggg ctgcaccggc tgatatgacg aagcggcgcg aatggtccac ttttccggcg
     2401 ataggaggtg tgcatggcgt atgccctgga ggcgagcgag gcggccgctg gcggactgaa
     2461 acgtgtcgcc ctggaacagc tcgaccaggc gctcgccagt gcccgcaacc ccggcacgca
     2521 acgcgacgag gcggtccacg atgtccgcaa gtgcatgaag aaactccggg cgatccttcg
     2581 catggcgcgt gaagagctgg gggagggggt attcaaacgg gagaacgcct gtttccgcga
     2641 cgcagcgcaa cgcctgtccg gtttacggga ggcagcagtg cggatggcga cggcggagaa
     2701 gctccagacg catttcggcg ggcaggatag cggcctgttc ataccgttga tcagcaacct
     2761 gcaggcgttg gccgagcagg cccggcgaga acacctggat gagagcgatg ccctgtgcga
     2821 agtcgcggcg atgctggacg gcgcacgcgc ccgcgtggac ggctggccgc tctcgggccg
     2881 gggattctcc gctgtcgcca atggcgtgcg ccgaatctat cagcagggcc gtaaggggct
     2941 ccgcctggcc cggaaaaaag cgaccgacga gcaatttcac gaatggcgca aggcggtgaa
     3001 atacctgtgg taccatggcc gcctgctgca atcgatctgg cccgccatga tgagcgtgct
     3061 cgtcgaggag cttgacgtgc tgggcgacac gctgggacaa gaccacgacc tggccgattt
     3121 gcgggtggcg ctcatccaga caccggcact ggcaggcgcc ccgcctgcgt ttgagacagt
     3181 cctgcatctg ggcgcccagc gccaggatga gctgcgccgg gcggcacttt ccctgggtgc
     3241 gcggctctac gcggagaaac cgcgcgcatt cgtggaccgg ctgggcgtgt actggaaagt
     3301 ctcacacgcg ccgcctgccg gttgatcggc ctttcaaagg cttcaggcct tgcccgggca
     3361 aagtgaaagg gtatcgcgcc tcatgccatt atgcccggtg gctgtttatt ccgggatagg
     3421 tagacgtgag agtgcccgcc gccggcagat aacagctgtg cacgtaatcc atcaccattc
     3481 gcgacgcact gaaccgccac ggcagggtgc gcagcgcatg tttctgcatg gcgacccagt
     3541 ctcggggaat accgtccgag tcacgcgcgt aatacagggg cgccacctca ttctccagac
     3601 aggcgaaaag tgcctcccgg tccgacgcgt cctgctgttc cgggtcgctg tgttcctcac
     3661 cagcgccgat ggcgaatccg ttgtgcccgt cgtacgcttc cgcccaccag ccgtcgagaa
     3721 cagacagatt aaggccgccg ttcaggacca ccttctgccc gcttgtgccg caggcctcct
     3781 ggggacgccg cggattgttc agccagacgt cgatcccctg taccaggtgc cggctcacgt
     3841 tgatgttgtg gttctcaata aacacaattt tgccccggaa ttccggcatc tgcgtaacat
     3901 cgtagacctg ttttatcagc gacttcccgt ttgaatcatt aggatgcgcc ttgcccgcca
     3961 gaatgaattg gaccggacgt tctttgtgat tgacgagccg gtcgaggcgc ggcagatcgc
     4021 cgaacagcaa cgtggcccgc ttgtacgtcg caaaccgccg cgcaaagccc acggtcagga
     4081 cgtcgtgttt caggcagtct tccggcgaca ggcagctcac acccgggccg ccgcgctgtg
     4141 caatctgatg ggccacgcag cggcggacat aatccaccag gctcgtcttg aggatctgat
     4201 gctgttccca gaattcgacg taatcgatct cttcgacatg ggcccaggtt tgagccaggt
     4261 gtgcgcggtc ggcccagtct tccccatggt accggctgta cagccgcacc atcgacatcg
     4321 ccagccacgt cggtgtatgc accccgttcg taatgtgata aatgggcact tcctccaccg
     4381 gccgcgacgg ccacaggccc cgccacatgc gccggctgac gcgctcgtga atcgccgaga
     4441 ctgcgttgcg gtagcgcgcc attttcagac cgagcacggt catgcagaac gtctcaccgt
     4501 catcgtgcgg gtcgacgcgc cccagcgcca gcagctcgtt ccctgaaatc cggagttgcg
     4561 cccgcagagg ctccagcatg cccaggagca tatcggccgg aaaccggtcg tgccctgctt
     4621 ccaccggtgt gtgcgtggta aacaccgtcc ggtttgacac gcgctcccgg acatcttcga
     4681 aggaccggtt ttcgcgttcc agcacttgtc tggcggcttc aagcgtggcg aacgcgctgt
     4741 gtccttcgtt caaatggatc acactcgggt gaatgcccag ggcttcaagg gcgcgcattc
     4801 ctccgatgcc cagaatcagc tcctgccgca gccgggtgcg ggcatcgccc ccatacaatt
     4861 gacccgtgag gccccggtcc tcctccgaat tttcctcgac gtttgtgtcg agcagaatca
     4921 atgaacagcg gcccacctca gcgcgccaga cgcgggccca ggtctggtcg ctcccgtttt
     4981 ccacaagtac ccgcaacggg cgcccctggc tgtccgttgc cacgtcaatg ggaagatcct
     5041 ccacctcggc ctcaagatat tcctcgcgtt gccagccttc ggcgtccagg cgttgattga
     5101 aatagccgta ggcgtagtac aggccgatgc ccaccagcgg gactcccaga tcggaggccg
     5161 cctgaagatg atcgcccgcg agcacgccca gcccgccgga gtagatcggc aacgactcgt
     5221 gaagcccgaa ttcagccgaa aaataggcca cggggttgag gcgcgtcggg ccaccggtcc
     5281 gcgagcccca ggtcgactgt tcggcaagat atccgttgag ttgatggaat gcgtgggtga
     5341 tccgtgtgtc aatcgcgagt tcggtgccgc gcttccgcag ggtttcagcg ggtacctgtt
     5401 tcaggaacgc gaccggattg tgattgacct cgcgccacag ctcaggatcg atgtcccgga
     5461 aaatcgcgat gacccgtggg tgccaggtcc agtaaaggtt gcgggccagc tctcgaaacc
     5521 gttgagagat ctccataatc ccgcctttct gctgcccgcg taaccggcaa tggcagcgtt
     5581 gttttgaggt tgcccggatc acatgcgggc agatacagaa atcagcagct cttgtatggt
     5641 caccttacat ggccttctcc tggacgtcaa tacgccgcag attatgaaac atcttgccct
     5701 gtcttcgccc aaacgcaaaa ccgccggcgc cgccctcgat tttgcattat ctgccgcccg
     5761 gccgtaggct atgcgtaata caaaggcggg ttttcaggtt caggcgggtt tcatgcccat
     5821 ggttccatgc cagaatgcag ggatcttcgt cacgctcctg ctgagcgcca tgccgcttgt
     5881 atcctcgcca ttctggttct gctctacatc gctttgggtc cgcccgtgcg gagaacgtgc
     5941 catcaacggg atgtcccgga cgtttccggg aacggaaagg agtctgctat gaagacagcg
     6001 gcatgtctgt tgttgtgtat ggtcgccggg agcccattgc tgctgcacgc ggcgaacgcg
     6061 gacactccgc ctggcccggc cagcgacgaa ctggcgaaga tgcgcgaggc cgcacccggc
     6121 aaggctactg ctgctcccgc gcaaccgcgc aaggtgctgg tctgctcgct ggtgacggcg
     6181 gactatgttc acagcgcgat cccgtacggc gaggcggccc tccagatcat cgcggagaag
     6241 acccaggcat tcagcgtcga atgctcaaac gacatcgcca tgtttgaacc cgaaaacctc
     6301 aaccgtttcg atgcggtctt tttcaacaac gcgtacggcg aactcttcct gcccgccggc
     6361 ttcgatgcgc tgcccgatgc gcagaaagag gcccttcgcg cgcgggccgc aacgctcaga
     6421 caaagtcttg ccgactttgt ccggaacggc aagggcattg ttgcgctgca cgccgggctc
     6481 gccatcttcg aggagtggga ggaatggggc gagatcatcg gcggccgctt tgataaccat
     6541 ccctggaaca aggtggtcac gattcgcgtc gaagagcccg cacatcccct ccttcaggcg
     6601 tttaaagaac cggagttcca ggtgcacgac gaaatctacc agttcacggg accgtactcg
     6661 cgcaatgcgc tccgtgtgct gctgtcactc gacctcgaaa agaccggcga accgaatgca
     6721 gcggaagtcc accgtaccga tggcgatttc gccctggcct ggatcaagcc ctacggcaaa
     6781 ggccgcgtgt tttattgtgg gcttggccac gaacacgacc tgttctggaa cccggccatt
     6841 ctgcgcttcc ttctcgatgg cacccagttc gccgccggcg acctgcaggc cgacatgacc
     6901 cccagcgcga accggtaaac aatggcgaac cgcaatttcc ggccgaaaca gccgcattcc
     6961 acctggatga ggctgcagaa ggcctgttcg cagccattcc gttcgcgatg accgcaatta
     7021 ccgcgttcga ttcaataaat atcccgggga attccatttt tctctctttg tatggcatag
     7081 ttcgtgtggc atagtctcca aaaatcgcga atacggggaa aacatcaacc tgtagggggg
     7141 cttccgggta aatacgccat attttgttgc caagacgggc ttcatttggt acactacgac
     7201 ctgtcaagtg attatcttgg ggaattgcca tgggttcgtc accgagactc tccgtcgatc
     7261 gcatcaggga aattgttggc gtcgttttta ttgctttcac tattttcctc tttctggctc
     7321 tggtcagtga tggatacgat ggcgatatcc gg
//
LOCUS       DAOPBQ010000121         7342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9131_length_7342_cov_3.299575, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000121 DAOPBQ010000000
VERSION     DAOPBQ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9131_length_7342_cov_3.299575"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..1275)
                     /locus_tag="PKW60_05485"
     CDS             complement(229..1275)
                     /locus_tag="PKW60_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98917.1"
                     /translation="MSNTQLIVGLPAGSLADPNRGGSLIELLKRAGFHVQGYDKGGPS
                     CFPLAGFLVGWDGRPQEFGSQLAIGEIDVAIGGDDWIRERTLEFKYEYGQEIALKKVL
                     ALDRGKVRIVIIGARETAAAGDDWLKNLLSTKPLVSMVSEMPYLALEWFRGKAEALGF
                     KESHWEFSVQKFKTPPRIERGIVIYETWGKTEAKVKHGSVDFGLEITQTGRALENYGL
                     CIFDELMTSEAGIWISPSLRDDPEKYDIARMFLLNLYGSIYAEDKVLVTFNTHKEDAG
                     KILEYLRDNRLFGEEPTINEGVNYTEYSIEVKTGSAGLPLPKVRYELAKLGATSIETI
                     PLESSIPELGVIDL"
     gene            1492..2145
                     /locus_tag="PKW60_05490"
     CDS             1492..2145
                     /locus_tag="PKW60_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPJ98918.1"
                     /translation="MIDAYIFDMDGTLLDSEILWVEAIEKCLVDFGFPVSHEEAVEIV
                     YGVSWRDIYEDLRRRFPGFSLGYQQFVSVVDPIFGTLRHTRDIRIHSSVELLQTLARD
                     YPVCIVSGSNTAGVNDGIDMMGIRQDIRFFLASEDYSPGKPDPACFLMAAERLEVDPA
                     RCLVFEDSQVGVQAAKAAGMHCVALSRPGRPRQDVSLADWVLDDLAAFSVRDYCPAS"
     gene            2203..5001
                     /locus_tag="PKW60_05495"
     CDS             2203..5001
                     /locus_tag="PKW60_05495"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPJ98919.1"
                     /translation="MKALPKIILVAGVTALAHLATDSGVWAGEQPWNLVQARLENGLT
                     VVTHEDFSNPLVSVQVWYHVGSKDEPETRQGMAHLVEHLMFRGSAAVGDYQHGDLIQA
                     AGGNTNAYTYFDHTAYVNTVPVAEFDLALWLEAERMAFLDVRDDAFETERNVVEEERR
                     TANLNAPYGTLPERVMAALFEQHPYRWLPIGTLAHLRATTPTDVRRFWDIYYVPNNAT
                     LVIAGAVTHESALAAARRYFGWIPRGAEPPRVRGKEPPQTSERDLCLPERRGPVPLAG
                     YVYRGVPASHPDAIPLRMVLWLLAHGEDSRMYRDLVRERGLCVVLLTQDFCLEQDGVA
                     GFVGALHPVRYYLGKLNPLGRPGDAIFRAFDRHIAEFQRNGPTPGELEKVKQQFRRAA
                     ASGCLTVEQRAWKFGEAAILHGDPDWDNREAAAVEAVTAEDLRRVASTCLTAERRTRV
                     RVLPDRSFNYEPGAGVDLAPPPFAEAPWHKAAWTRPVGFPATPPVSGTPPEIPTIPAQ
                     KHELANGLNVVVIPDRESPLFLAAMGFREGPRAEPRPGATALALKALSKGTENRSADE
                     LAQFIEQRALMVQEVVVMDASLLAVSGLSETGPDCLDLLAELVISPTFPRSELRQLRR
                     QERVMLRYAHSDPSYRADIELRRRLFGADPYARAATGGPGDVRRLNRRDVTHWWQAIA
                     RPDDAVLYLGGDLDPETAVRWAEQAFSAWTAPTISRETVSAALPPSPEPARVYLTSIP
                     GAVQSQIRIGQLAFAPDHPDYFPAQLFSEIYGGSFDSRLNKALRGAQGRTYGAYGGFT
                     FFKRGGYFASSVSTQTEQTSQALASMLDVLEGMRAAPPSPDELQRAVSHLTGRTILDL
                     ETFAEVISWLWYLELMELPSDYLARSYAAFAAMTPEDILRVAQEQVDPSHLTIAVTGD
                     AEAIEAGLQAFGTVTRSR"
     gene            5560..6108
                     /locus_tag="PKW60_05500"
     CDS             5560..6108
                     /locus_tag="PKW60_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HPJ98920.1"
                     /translation="MAVTILRIVLGVALGAGLGAVMGSTRSCETGGCPLTANPRRGAI
                     YGGLMGLLIAFAFAPASKPVIEADSGIVEVTSSAQFNELVQASDKPVFAYFHAPWCGA
                     CRQSGPIVNELARNHAGAVLFLGIDTDKNSELAREHAVQYLPTSLVFRGGREAARIVG
                     TFDKEQVLRELLTPDSPQAGPS"
     gene            6199..7167
                     /locus_tag="PKW60_05505"
     CDS             6199..7167
                     /locus_tag="PKW60_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98921.1"
                     /translation="MRKLVHLACIACLLSCVAATAQDSAPVPPQPAFANATWTLITRE
                     AAFSPRDTAEGVVYDNKMWLSNGYYHDNVLSRDLWTSTDGVTWTLVNEATPYDGYSEM
                     TVYDGKMWAIKGSVWTSTDGVDWTRVAETTPFGVRGYGEVVVHDGKMWHLGSGADVWW
                     TTDGVNWTCAVENAPFGHRTASAVAVYEGKLWLMGGYIQQTSDPPEKLYTQYTTYNDV
                     WTSTDGVTWECVLEHAPWSPRMWFIAQPYAGELWLIGGFSNQHSANLGDVWRTRDGKQ
                     WTRLEPSTTVAPRHEPTCYVYENSLWVVAGNTWPVVNDVWRLTFDK"
BASE COUNT         1376 a         2318 c         2227 g         1421 t
ORIGIN      
        1 catcgcgcca tggcgcggca gatggcggtg tttcgtggaa gctggcaccg gaagaactgg
       61 gtcaggcctg tgccgatatt ctgcgcacgg ctccaggcac cctgatcagc gagctggaga
      121 tccgccccct gagccgtccg ccgaaaccgt agccgggcgc atgcgacgaa cagaggaatc
      181 ctgtcgctcc gattccgggg tctcgtgcgg tcaggcggct ggctcgtgct acaagtcgat
      241 gacacccaac tcggggatgg acgactcgag cgggatggtc tcaatgctgg ttgcgcccag
      301 tttggccagt tcgtagcgca ctttcggcag cggcaggccg gcgctgccgg tcttcacctc
      361 aatgctgtac tcggtgtaat tcacgccttc attgatcgtg ggctcttccc cgaacaggcg
      421 attatcgcgc aggtattcaa ggattttccc ggcgtcttct ttgtgggtat tgaaggtcac
      481 cagcaccttg tcttcggcgt agatggagcc gtatagattc agaagaaaca tccgcgcgat
      541 atcgtacttt tcgggatcat cgcgcaacga cgggctgatc cagatccccg cctccgaggt
      601 catcagttca tcgaagatac acagtccgta gttttcgagt gcgcggcctg tctgggtgat
      661 ctcgagacca aaatcgacgg agccgtgttt caccttcgcc tccgttttgc cccaggtctc
      721 gtaaataaca atgccgcgct caattcgggg aggcgtcttg aacttctgca cggaaaattc
      781 ccaatgcgac tccttgaaac cgagcgcctc cgccttgccg cggaaccatt cgagcgccag
      841 atacggcatc tccgacacca tgctgaccag cggcttagtc gacaggaggt ttttgagcca
      901 gtcgtccccg gcagcagccg tttcccgggc gccgatgatg acaatgcgca ctttgccccg
      961 gtcgagtgcc agcacttttt tcagcgcaat ctcctggccg tattcgtatt tgaattcgag
     1021 cgtccgttcg cggatccagt cgtcgccccc gatcgccacg tcgatctcgc cgatcgccag
     1081 ttgcgacccg aattcctggg ggcgcccatc ccagccgacc agaaatcccg ccagcgggaa
     1141 acagcttgga ccgcccttgt catagccctg gacatggaat ccggcacgct taagcaactc
     1201 gatcaggctg ccgccgcggt tgggatccgc cagggaaccg gcgggcagac caacgattag
     1261 ttgcgtgtta ctcatgatgc gcctctggtc tgttgatggt ttcggtattg ttcgtcagga
     1321 aaatcatctt atacgcattt gcccccgtcg agatcaagaa cggggtgggc aggacggcat
     1381 ttcaagccat ttgctcatct tcccggagcg cacaaattct tgcgccaccc ctgcccgatt
     1441 tactatgatc tgccgggagt aacctcgcat tagcaaaggc catttcaccc gatgatagac
     1501 gcgtacattt ttgatatgga tgggacactg ctggactcgg agattctctg ggtcgaggcc
     1561 atcgaaaagt gtctggtgga tttcggattt ccggtgtctc atgaagaggc tgtcgaaatc
     1621 gtgtatggcg tttcctggcg cgatatctat gaggacctgc gccggcgctt tccggggttc
     1681 agcctgggtt atcagcagtt tgtgtcggtg gtcgacccca tcttcggtac gttgcgccat
     1741 acgcgtgata ttcgcatcca cagctcggtt gagctcctcc agacgctcgc ccgggattat
     1801 ccggtgtgta ttgtatcggg ctccaacacc gcgggcgtga atgacggcat cgatatgatg
     1861 ggcattcgcc aggatatccg gttctttctg gcctcggaag actattcgcc cgggaagccc
     1921 gacccggcgt gcttccttat ggcagctgaa cgcctcgagg tcgaccccgc ccggtgcctg
     1981 gtgttcgagg attcccaggt tggcgttcag gcggccaagg ccgccggcat gcattgtgtc
     2041 gcgctgtcac ggcccggacg gccccgccag gacgtatcac tggccgattg ggtgcttgac
     2101 gatctagccg cattttccgt gcgcgattac tgccccgcgt cgtagcgcaa cagaggccgg
     2161 acggccagtc aggcagcgcg gcgcatggct tcggcaatca caatgaaagc actgccaaag
     2221 atcattctcg tggcgggcgt gaccgcgctg gcgcatctgg ccacagattc cggtgtctgg
     2281 gccggggaac aaccctggaa cctcgtccag gcgcggctgg agaacggtct caccgtcgtc
     2341 acgcacgagg atttctcgaa cccgctggta tccgttcagg tgtggtacca cgtggggtcg
     2401 aaagacgagc cggaaacccg ccagggcatg gcccacctgg tcgaacatct catgttccgg
     2461 ggcagcgcgg cggttgggga ttaccagcac ggcgatctca tccaggccgc gggcggcaac
     2521 accaacgcct acacctattt cgaccacacc gcgtacgtta atacggttcc cgtggctgag
     2581 ttcgatctgg ccctctggct cgaagccgag cgcatggcgt ttctggatgt ccgcgacgac
     2641 gcgttcgaga ccgagcggaa cgtcgtcgaa gaagagcgcc gcaccgccaa cctcaacgcg
     2701 ccgtatggaa cccttcccga gcgcgtcatg gccgcgctgt tcgaacagca cccgtaccgc
     2761 tggctgccca ttggcacact cgcccatctg cgcgccacga cgcccaccga cgtccgccgg
     2821 ttctgggaca tctattacgt gcccaacaat gccaccctgg ttatcgccgg agccgtgaca
     2881 catgaatccg cgctggcggc cgcccgccgg tatttcggct ggatcccgcg cggagcagag
     2941 ccgccccggg tccgcggcaa ggaaccgcca caaaccagcg aacgggatct atgccttccc
     3001 gaacgccgtg gcccggtgcc gctggccggt tatgtctatc gcggcgtccc cgccagccac
     3061 cccgacgcga ttcccctgcg tatggtgctg tggctcctgg cccacgggga agacagccgg
     3121 atgtaccggg accttgtgcg cgagcgcggt ctatgcgttg tgctcctgac ccaggatttc
     3181 tgcctggagc aggacggcgt cgcgggattc gtgggggcat tacaccccgt gcggtactac
     3241 ctcggcaagc tcaaccctct gggcaggccg ggtgacgcca tattccgggc ctttgaccgc
     3301 catattgccg aatttcagcg caatggcccg actcccggcg agttggaaaa agtgaagcag
     3361 cagttccggc gggcggctgc ctcgggatgc ctgacggtgg aacaacgcgc gtggaaattc
     3421 ggcgaggcgg ccatcctgca cggcgatccc gactgggaca atcgagaagc ggcggccgtc
     3481 gaagccgtca ccgccgaaga ccttcgccgg gttgcctcca cctgcctgac agcggaacgg
     3541 cgcacccggg tgcgggtgct gcccgaccgg agcttcaatt acgaaccggg tgcgggcgtc
     3601 gacctggcgc caccgccctt tgccgaggcg ccctggcaca aagctgcctg gacgcgcccc
     3661 gtcggatttc ccgcgactcc gcccgtcagc ggcacgccgc cggaaatccc cacgataccc
     3721 gcgcagaaac acgagcttgc caatggcctg aacgtggtcg tgattcccga ccgcgaatcg
     3781 ccgctcttcc tcgccgcgat gggtttcagg gaagggcccc gggcggaacc caggccgggg
     3841 gcgaccgccc tcgctctgaa ggcgctgagt aagggcaccg aaaaccgctc cgccgatgag
     3901 ctcgctcaat tcatcgaaca gcgcgcgctg atggtgcagg aagtcgttgt gatggacgcc
     3961 tccctgctcg ccgtcagcgg gctcagcgag accggcccgg actgcctgga cctgctggcc
     4021 gaattggtaa tctcccccac attcccccgc agcgaattgc gtcagctccg gcgccaggag
     4081 cgcgtgatgc tccgctacgc gcacagcgat ccgtcgtatc gcgccgacat cgagttgcgc
     4141 cgccggcttt tcggagccga cccctacgcc cgcgctgcca cgggcggccc gggcgacgtt
     4201 cggcgtctca atcgccgcga tgtgactcac tggtggcagg cgatcgcgcg gccggacgat
     4261 gccgtcctgt accttggcgg cgatcttgac ccggaaaccg cggttcgatg ggcggaacag
     4321 gctttcagcg cgtggaccgc gcccaccata agccgggaaa ccgtctccgc agccctcccg
     4381 ccctctcccg aaccggcccg ggtgtacctc acctcgattc cgggcgccgt gcagagccag
     4441 atccgaatcg ggcaactcgc cttcgcgccg gaccatcccg attacttccc ggcgcagctg
     4501 ttcagcgaga tctacggcgg atcattcgac agccggctca ataaggccct gcgcggggca
     4561 cagggccgga cctacggcgc ctatggcggg ttcacctttt tcaagcgcgg aggttatttc
     4621 gcaagcagtg tctcgacgca aacggaacag acctcgcagg ccctggcgtc catgctggac
     4681 gtgctggaag gcatgcgcgc cgcaccaccc tccccggacg agcttcagcg ggcagtatcc
     4741 catctcacgg ggcggacgat cctcgacttg gagacattcg ccgaggtcat tagctggctg
     4801 tggtatctcg agttgatgga actgccatcc gactacctgg cccgctcgta tgcagcgttt
     4861 gcggccatga ccccggaaga catcctgcgg gtcgcccagg aacaggtgga tccatcccat
     4921 ctcaccatcg cagtcaccgg cgatgcggag gccatcgaag caggccttca ggccttcggg
     4981 accgttactc gttccaggta gactgttacg caacggcacg caacgctccg caacgttgcg
     5041 tttccggaag tcgccattta cacgcgaaaa cttacgccgt ctgccaaatt cacataaact
     5101 tttatgtact aataatttaa tgatgagtgc ccaacaataa gcccgacttg gccccggaat
     5161 tgcttctctt tattttgata ccataaacta cgaccagcag gagtggaaac atgccgattt
     5221 tctccatcaa gtgtaacgaa tgtaacgcca tcgatgaagt actggtgccc agtgcatccg
     5281 tccagacgcc cccgtgcagt gtgtgtggca gctccgacac cgagcggatg gtatcccgtc
     5341 cggcggtctg cacggggagc agcagcggcg gcagctgccc caccggcacg tgcccattcg
     5401 caaactagcg catattgaca acaaattgaa gtcaatatat gcaaaaatat atgcaaaaat
     5461 tgacctgcag gaaataaatc cgcgttctcg agcactccaa tatggtttgg atagccggca
     5521 agcgaggaaa acggaacgct ccggatagga gtcgagcaca tggcagtaac gatacttcga
     5581 attgtattgg gcgtggccct gggcgcgggg ctgggcgccg tcatgggcag cacgcgctcg
     5641 tgcgagaccg gcgggtgtcc gctgaccgcc aacccgcggc ggggagcgat ctacggcggg
     5701 ctgatgggat tgctgattgc ttttgccttc gcgcccgcat cgaagcccgt tattgaagcg
     5761 gattcgggca ttgtcgaggt gacgtcgtct gcacagttca atgaactggt acaggcctcg
     5821 gacaagccgg tatttgccta ttttcacgcg ccctggtgcg gcgcatgccg ccagtcgggc
     5881 ccgatcgtaa atgaactggc ccgcaaccac gctggcgctg tgctcttttt aggtatagac
     5941 acggacaaga attctgaatt ggcgcgggag cacgcggttc aatatctgcc cacgtcgctt
     6001 gtgtttcgcg ggggccgtga ggcggcccgg attgtgggca cgtttgacaa ggaacaggtg
     6061 ctgcgtgaac tgttgacccc cgattcaccg caagccggac cctcttgacc ccctcgcacg
     6121 cctctccccg gctttgtggt agagtgaagg cgggatcctg cccccggatc ccgccttttt
     6181 cacgtctgga ggaagctcat gcgaaaactc gttcatctgg cctgcatcgc gtgtttgttg
     6241 tcgtgcgtgg cagcaacggc gcaggactca gcacccgttc ccccgcaacc ggcgtttgca
     6301 aacgccacgt ggaccctcat aaccagggag gccgcgttca gtccgcgcga caccgcagaa
     6361 ggggtcgtgt atgacaacaa gatgtggctg agcaacggtt attaccacga caatgtcctc
     6421 tcgcgcgacc tgtggacctc tacggatggc gtgacctgga ccctggtgaa tgaggcaacg
     6481 ccctacgacg gctacagcga gatgacggtg tacgacggga aaatgtgggc catcaaaggg
     6541 tcggtatgga cctcgaccga tggcgtcgac tggacccggg tggcggagac cacgcctttc
     6601 ggcgtgcgcg ggtacgggga agtggtggtg cacgacggca agatgtggca tctcggcagc
     6661 ggcgctgacg tgtggtggac caccgacgga gtaaactgga cctgcgcagt ggaaaacgcc
     6721 cctttcggac accgcaccgc cagcgccgtc gcggtgtatg aaggaaaatt gtggctgatg
     6781 ggcggctata tccagcaaac gagtgatccg cccgaaaagc tgtacaccca atacacgacc
     6841 tacaacgacg tatggacctc gaccgacggc gtgacatggg agtgcgtgct ggagcatgcc
     6901 ccctggtctc cgcgcatgtg gttcatcgcg cagccgtatg cgggcgaact ctggctcatc
     6961 ggggggttca gtaaccagca tagcgcaaac ctgggcgacg tctggcgcac ccgcgacgga
     7021 aagcaatgga cccggctgga gccttcgacc accgtcgcgc cgcgccacga acccacttgc
     7081 tacgtgtacg aaaacagcct gtgggtcgtg gcaggcaaca cctggcccgt ggtcaacgat
     7141 gtgtggcggc tgactttcga caagtaaggc cgccgcttct tccccgcgcg ctcgctcgtc
     7201 cggcgggaaa ccaccccccg gattgcggta ttcttgtggg atggaacgac caccctttgc
     7261 atttgccgaa ccggactatc cactggcgcc ggacccccga cgcggtatcg cgtcagcggc
     7321 gcccgtggat agtccggttc gg
//
LOCUS       DAOPBQ010000122         7306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9183_length_7306_cov_4.399945, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000122 DAOPBQ010000000
VERSION     DAOPBQ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9183_length_7306_cov_4.399945"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PKW60_05510"
     CDS             <1..743
                     /locus_tag="PKW60_05510"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPJ98922.1"
                     /translation="KTNCAGYSRSFRTNARSRHHAVGGAGVLLLVLLLPGTVPAQDAA
                     PVALSTITSPGTATVETPPDRADFWLYFRLTGNSVQEAAEEVKTTVVEILRLLKDREI
                     APLETEVTGPSIPDANTPVVTFAFRLRFTVGRLVAGVEEPVQRLAAICDALRAAARET
                     GAVLTGPLLKVSNPNAIERTAVMRAMENALPNAEAAAHIMATRIDTIFDVRILEIAWN
                     QDPEHGADQPDTERVTCSARVEVVYVLN"
     gene            complement(757..1515)
                     /locus_tag="PKW60_05515"
     CDS             complement(757..1515)
                     /locus_tag="PKW60_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPJ98923.1"
                     /translation="MPDPADIFSDSGMRERFARFFRYAQVGRCVNSVTHDVNNYLGAI
                     LAYAELVALEENQTNESQRMLAEIKVAVQRASRLVSSLTDIARRERPDVRVVAPRDIL
                     ERVLDLRRYDLRVAHIELEVTGGEELPQISVELPRLQQCLIYLLANAIEAVEGKEDRR
                     IRLSVSAGEGTVEFRFWNAGGAIPEDVRARLFEPFFTTRGGVHLGLGLWAARHNLRAL
                     QGDVSYDPVLGFTVAVPQETGGSALRPQHPEPGA"
     gene            complement(1637..2866)
                     /gene="icd"
                     /locus_tag="PKW60_05520"
     CDS             complement(1637..2866)
                     /gene="icd"
                     /locus_tag="PKW60_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPJ98924.1"
                     /translation="MFKYIQVPPGEPIAVSRDGVLQTPDKPIIAFIEGDGTGPDIWHA
                     SKMVFDAAVRHCYGDQRAIAWMEIYAGEKANQLCGSYLPDETLEAIEKYHVAIKGPLT
                     TPVGGGFRSLNVTLRQRLDLFACVRPVRYFPGVPSPVKEPEKVDMTIFRENTEDVYSG
                     LEVQAGSGEARKLIAFCEEAFGWKIRPDSGIGLKPVSVEGSKRLIRAAIRYALKHGRK
                     SVTLVHKGNIMKFTEGAFRQWGYDLVRDEFADVAVSWDDCGGKPGGKLLVKDAIADIF
                     LQQILTRPTEFDVIATLNLNGDYMSDALAAQVGGIGIAPGANINYETGMAIFEATHGT
                     APKYAGLDQVNPSSVILSGSMMFEHLGWTEVSNAIVRSMMRTFSNKTVTYDFERLMTG
                     ATLLKCSEFGKAIIDNL"
     gene            complement(2925..4502)
                     /gene="serA"
                     /locus_tag="PKW60_05525"
     CDS             complement(2925..4502)
                     /gene="serA"
                     /locus_tag="PKW60_05525"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPJ98925.1"
                     /translation="MSKILALDNLCESGLEVFQKEGFEVDVKPPQKPEELAAIVGEYD
                     GLVIRSATKVTAEALAHSGKLKVIGRAGVGTDNVDKVEATKRGIVVMNVPGGNTISTC
                     EHTLALLLALCRNIPKAHQSMREGRWDRKLFMGTEVRDKTLGIIGVGRIGGEVAKRAQ
                     AFEMKVLAFDPILTRLKAEALGVELVSLDEMIERADFISIHAPKSDKTLNMIGYEQLK
                     RMKPTCRIVNCARGGIVNEADLARALKEGVIAGAALDVYTSEPFENNPFAGLDNVIMT
                     PHLAASTDEAQLTVAVDVAKQMVEYLKSGTITNAVNVPSLDGETRKKLSPLLYLAERL
                     GTFLSGYMKGTPKAIRIDYSGALPVTDTYPLTVAVLIGFLKPLVESVNEVSAPSLLED
                     HGIDFSETRRADVPDYNFLVGVTVETDEEKHHVAGTLFGNDDPRICVIDGTRVNAVPE
                     GHMVVCRNEDKPLIIGRVATIIGDAGVNIANLVLGRDERGGRAATILNIDDPLSDDVM
                     EKIRAVPYVKEARQLKL"
     gene            complement(4924..5061)
                     /locus_tag="PKW60_05530"
     CDS             complement(4924..5061)
                     /locus_tag="PKW60_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98926.1"
                     /translation="MHRCALCNEHIEEVDIQFNEVAILDDEYWHLDCYAEYFGEVFEE
                     A"
     gene            5502..>7306
                     /gene="alaS"
                     /locus_tag="PKW60_05535"
     CDS             5502..>7306
                     /gene="alaS"
                     /locus_tag="PKW60_05535"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPJ98927.1"
                     /translation="MKADEIRSSYLEFFRERGHVVERSDKIVPSNDPTLLFSSAGMVQ
                     FKPYYTGEVPVPYRRAATAQKCLRAGGKANDLDEVGKTARHMTFFEMMGNFSFGDYFK
                     REAIQWAWEYSTQVVKIAPEQIWISVYEDDDEAANIWETEIGIPSGKIVRLGVKDNFW
                     GPAGDTGACGPCSELYLDRGEAFGPGPLGADGDSRFMEYWNLVFPQFDQQPDGSRPPL
                     KNRGIDTGMGLERMAAILQGKETVFDVDHIFPIIQNAESLSGATYTANPTPFRVIADH
                     IRALSFMIADGVLPGNEGRGYVERRLLRRAARFGREIGLERPFLFELSRTVVDLMAHQ
                     YPELKERQLQIEKIIQTEEERFASTLARGMDLLEDVFAELAKTGSSAVPGAELFRLHD
                     TFGFPLDLATDIAEDRGFTVDREGFEAAMRKQRELGRSAWAGTNEHEISPIHHKLREE
                     LDETKFVGYDTLSSEATIQALLRDGARVDALADGEEGDVVLDVSPFYAESGGQIGDTG
                     TLESTAGSANVITTQVPLEPLRLHRVKVTQGSLSVGDKVTAAVDGAPRMATACHHTAT
                     HLLQAALQESLGAHVRQAGSQVSPERLRFDFTHFEG"
BASE COUNT         1441 a         2214 c         2183 g         1468 t
ORIGIN      
        1 cgaagacgaa ttgcgccggt tattcccgtt ctttccggac aaacgcacgt agccgccacc
       61 atgccgtggg gggcgccggc gttttgctcc tggtgttatt gctgcctggg acagtgccgg
      121 cccaggatgc agcgcctgtt gccctgtcta ccatcacatc gcccggcacg gcgactgtcg
      181 aaaccccacc cgatcgcgct gatttctggc tctatttccg attaacaggc aacagcgtcc
      241 aggaggcggc cgaagaagtc aagactacgg tggttgagat cctgcgcctg ttgaaagacc
      301 gggagatcgc gcccctcgaa acggaggtca ctggaccgag tatccccgat gccaacacgc
      361 cggtggtcac gttcgcgttc cggctccgct tcactgtggg caggctggtg gcgggcgtcg
      421 aggaacccgt gcagcgcctg gcggccattt gcgacgcgct gcgcgccgct gcccgggaga
      481 caggcgccgt gctgaccggg ccgttgctca aggtctccaa ccccaatgcg atagaacgca
      541 ccgccgtcat gcgtgcaatg gagaacgccc tgcccaacgc ggaagccgcc gcgcacatca
      601 tggccacgcg catcgacacg atctttgacg tccggatcct ggagatcgca tggaatcagg
      661 acccggaaca cggagcagat cagccggata ccgagcgcgt gacctgctcg gccagggtgg
      721 aagtcgtcta cgtgctgaat tgatggcgcg gcgcggtcac gcgcccggct caggatgttg
      781 tgggcgcaaa gcgctgccgc cggtttcctg cggcactgcc acggtaaatc ccagcacggg
      841 atcatagctt acgtcgccct gaagcgcgcg gaggttgtgg cgggcagccc acaagcccag
      901 cccaagatgg acgccgcccc gggtcgtgaa aaagggttca aacaaccggg cgcgcacatc
      961 ctccgggatg gcgccgcccg cattccagaa tcggaattca acagtccctt ccccagcgga
     1021 gaccgacagc ctgatccgcc ggtcttcctt gccttcgacg gcttctatgg cgttggccag
     1081 caggtagatt aaacactgct gaagtcttgg caactcgacc gaaatctgcg gcagctcttc
     1141 cccgcctgtg acttcgagct cgatgtgggc cactctgaga tcatagcgcc gaagatcgag
     1201 caccctttcg agaatgtcgc gcggcgccac gacgcggaca tccggccgtt cccggcgggc
     1261 aatatcggtc agactgctga ccagacggct ggcgcgctgc accgcaacct tgatctccgc
     1321 gagcatgcgc tgagattcat tcgtctgatt ctcttcgagg gccaccagtt cggcataagc
     1381 caggatcgcg ccgagatagt tgttcacatc gtgggtcacg ctgttgacac agcggcccac
     1441 ctgggcatag cggaagaatc gcgcaaaccg ctcgcgcata ccgctgtcag aaaaaatgtc
     1501 ggccgggtcc ggcactccag acgttccttt atcatcctgt tgccccgcgc cactcgcgca
     1561 gggctgtcgc cgggctgagt ccggccggcg cgccgatggg ttcaggtgcc ggccgggctt
     1621 tggcgcgatc tactgcttac agattatcga taattgcctt gccgaattca ctgcatttca
     1681 gcaacgtcgc gcccgtcatc agccgttcga aatcataggt gaccgtctta ttggaaaagg
     1741 tgcgcatcat ggagcgaaca atcgcattgg atacttcggt ccagcccaga tgctcgaaca
     1801 tcatgctgcc ggacaggatc acgctgctgg gattgacctg gtcgaggccg gcgtatttcg
     1861 gcgcggtgcc gtgggtggct tcgaaaatgg ccatgccggt ttcgtagttg atattcgccc
     1921 cgggcgcaat gccgatgccg cccacctggg cagccagcgc gtcactcata tagtcgccgt
     1981 tcaggttgag ggtggcgata acgtcaaatt cggtggggcg cgtcaggatc tgctgcagaa
     2041 agatgtcggc gatggcgtct ttgacgagca gtttgccgcc cggtttgccg ccgcaatcgt
     2101 cccagctcac ggccacatcg gcgaattcat cccggacgag gtcgtatccc cattgccgga
     2161 acgccccttc cgtgaatttc atgatgttgc ctttgtggac gagcgtgacc gatttgcgcc
     2221 cgtgtttcag cgcataccgg atcgcggccc gaatgagccg cttcgaaccc tccacgctta
     2281 ccggcttgag gccaatgccc gaatcggggc ggattttcca gccgaaagcc tcttcgcaga
     2341 acgcgatcag ttttctggcc tcgccgcttc ccgcctgcac ttccagtccg gagtacacgt
     2401 cttccgtgtt ttcgcggaaa atggtcatat cgaccttttc cggctccttg acggggctgg
     2461 gcaccccggg gaaatagcgt acaggccgga cacaggcaaa caggtcgagg cgctgccgca
     2521 gggtgacgtt caggcttcga aatccgccgc ccacgggcgt ggtcagcggc cccttaatcg
     2581 ccacgtgata cttctcaatg gcttccaggg tctcgtccgg cagatagctc ccgcagagct
     2641 ggttcgcctt ttcgcccgcg tagatctcca tccaggcgat cgcgcgttgg tctccatagc
     2701 aatgccggac ggcggcatcg aagaccattt tcgaggcgtg ccagatatcg gggccggtac
     2761 cgtcgccttc gatgaacgca ataatgggtt tgtccggcgt ttgaaggacg ccgtcacgcg
     2821 agacggcgat tggctcgccg ggcggaacct gaatgtactt aaacatgctc tctctatgct
     2881 cccgctgagt ataacgcggg catgctggcg gcgcagggcg ccgcctacag tttcaactgc
     2941 cgcgcttctt tcacgtacgg caccgctcga attttctcca tcacgtcgtc cgacagcggg
     3001 tcgtcgatat tcagaatggt cgctgcccgg ccgccgcgct catcgcgccc caggaccagg
     3061 ttggcgatgt tgacgccggc gtcaccgata atggtggcca cacggcctat gatgagcggc
     3121 ttgtcttcgt tgcggcacac caccatgtgc ccttccggaa ccgcatttac gcgggttccg
     3181 tcaataacgc agatgcgggg atcgtcattc ccgaacaggg tgcccgcgac atggtgtttt
     3241 tcctcgtcgg tctcgaccgt gacgcccaca aggaaattgt agtccggcac atcagcccgg
     3301 cgggtttcgc tgaagtcgat gccgtggtct tccagaagac tgggagcgct cacttcgttc
     3361 acgctctcca ccagcggttt caggaaaccg atcaggacgg ccacagtcag cggataggtg
     3421 tcggtgacgg gcaacgcgcc gctgtagtca atgcgaatgg ctttgggagt ccctttcatg
     3481 tatcccgaca ggaacgtgcc cagacgttca gccaggtaca gcagcgggga gagtttcttg
     3541 cgggtctcac catcgaggct cggcacgttg acggcgttgg tgatcgtacc ggacttcaag
     3601 tactcgacca tttgcttcgc cacatcaact gccactgtca gctgcgcttc gtcggtcgat
     3661 gcggcaaggt gaggcgtcat aatgacgttg tccagcccgg cgaacggatt gttttcaaag
     3721 ggttcggagg tatagacgtc gagcgcggcg ccggcgatca cgccttcctt gagcgcccgg
     3781 gccaggtccg cttcgttgac gatgcccccg cgggcacaat tgacaatccg gcatgtgggc
     3841 ttcatccgtt tgagctgctc atacccgatc atgttgagcg tcttgtcgct cttgggcgcg
     3901 tggatcgaga tgaaatcggc ccgctcgatc atctcgtcga gggaaaccag ctcgacgccg
     3961 agtgcttccg ctttgagccg ggtgaggatg gggtcgaacg ccagaacctt catctcgaaa
     4021 gcctgtgcgc gtttggcgac ctcgccgcca atccgcccca cgccgatgat gccaagggtt
     4081 ttgtcgcgca cctccgtgcc catgaagagc ttacgatccc agcgcccctc acgcatggac
     4141 tggtgcgcct tgggaatatt gcggcaaagg gccaggagca gtgcaagcgt gtgttcgcag
     4201 gtcgagatgg tattcccgcc ggggacgttc atcacgacaa taccgcgctt cgtcgcttcc
     4261 actttgtcga cattatccgt gccgactccg gcgcggccga tgactttcag cttgccggaa
     4321 tgggcgaggg cttcggctgt caccttggtc gcgcttcgaa tgaccaggcc gtcgtattcg
     4381 ccgacaatag cggccagctc ctcaggtttc tggggaggct ttacgtccac ctcgaagcct
     4441 tccttctgga atacctcaag acccgattcg caaaggttat caagagcgag gattttagac
     4501 atggtttcct ccgtgccgga atgaattccg cgcagctaca gcccgccccc tacgcattga
     4561 cccacagttt gactcacggt aaatcagcag ccttgaacac catttccgca gccgccgatg
     4621 tacctgtgcc gattgaagcg ctgaatttac caaagcgcag aactgatgtc aatcagcgaa
     4681 gcgcgccccg gcctgatacg ccagtagcac gagaaaccgg acccaggcgc ccggaaacgg
     4741 ggtctggcgg gaaagatgcg cgccggcggg gtggcagccc gaaaaaagcc gctccgaaac
     4801 aaaaaccccg aagcggccgc taatggaatt cagatgtgag agcaaaaaaa atacccggga
     4861 gacctgactc ggcctgcctt agggtaatta agcaaaaaat gtgcttagtt gggggatacg
     4921 tgttcaggcc tcttcaaaga cctcgccgaa atactcggcg tagcaatcca gatgccagta
     4981 ctcgtcatcg agaatggcta cttcattgaa ttggatatcc acctcttcaa tgtgttcatt
     5041 gcacagtgcg cagcgatgca tggcgtaact cacctccctt ctgaagtgtt tctggtatgt
     5101 ccgtgcgatg ctgccatctt gggcctgagc attcggatgt ttcttcatcg cccggacgcg
     5161 tgacgcaatc gcgccgtgta tgccggactc gatacagagg aggatgttcg gccactcgct
     5221 atcggaactc cggcacaatc gggagatatg taccacatcc ggttgccggt gtcaagcttt
     5281 tttttaaata atctgatgtg ccccattcag gtagatccgg cgcaaatgca ggctgcaacc
     5341 tgcaatcctt tgatccgcaa aatgttagag ggtttcaatc ccggtgcatg gcgccggcgg
     5401 gatgcgggca tgccacattg aaaagatacc cccgtttccg atattttatg taatctttgc
     5461 ggtgccgccc gtgcgggcag agtcattaat ggagaataag cgtgaaagcc gacgagatcc
     5521 gttccagtta tcttgaattc ttccgcgaac gcggccatgt agtggagcgc agcgacaaaa
     5581 tcgtgcccag caatgatccg acgctcttgt tcagcagcgc gggtatggtg cagttcaagc
     5641 cgtattacac gggcgaggtg ccggtaccgt accggcgcgc cgccaccgcg cagaaatgcc
     5701 tccgggccgg cggcaaggcc aacgacctcg atgaagtcgg caaaaccgcg cgccacatga
     5761 cgttctttga gatgatgggc aacttctcgt ttggcgacta tttcaaacgg gaagcgattc
     5821 agtgggcctg ggaatactcc acccaggtgg tcaagatcgc cccggaacag atctggatct
     5881 cggtatacga ggacgacgac gaggccgcga acatctggga gacggaaatc ggcattccct
     5941 caggcaaaat tgtccggctg ggcgtcaaag acaatttctg gggcccggca ggcgataccg
     6001 gcgcctgtgg tccctgttca gagctctacc tggatcgcgg cgaggccttc ggtccgggcc
     6061 ccctgggcgc ggacggcgac tcccgcttca tggaatactg gaacctggtg ttcccgcaat
     6121 ttgaccagca gcccgacggc tcgcgcccgc cgctcaaaaa ccggggaatc gataccggaa
     6181 tgggcctcga acgtatggcc gcgattctcc agggcaagga aacggtcttc gatgtcgacc
     6241 acattttccc gatcattcaa aatgcagaat ccctgtccgg cgcgacgtat acggcaaacc
     6301 cgacaccttt ccgcgtcatc gcggatcata tccgcgcgct ttcgttcatg attgccgacg
     6361 gggtgctccc gggtaatgaa ggccggggct atgtcgagcg gcgcctcctg cggcgggcgg
     6421 cgcgcttcgg acgtgaaatc gggctggaaa ggccgtttct tttcgaatta tcgcgcacag
     6481 tggtcgatct catggcgcac cagtatcccg agctgaaaga gcgacagctc cagatcgaaa
     6541 aaatcatcca aaccgaggaa gaacggtttg cgagcactct ggcccgtggc atggaccttc
     6601 tcgaggacgt gtttgcggaa ctcgcaaaga cagggagcag cgcagttccc ggcgcggagc
     6661 tgttccgtct gcacgacacc ttcggctttc ccctggatct cgcgacggac atcgctgagg
     6721 atcgcggctt taccgttgac agggaaggct ttgaggcagc catgcgcaag cagcgcgaac
     6781 tggggcgcag cgcgtgggcg ggaacgaacg agcacgagat ctctccgatc caccacaaat
     6841 tgcgcgaaga actggacgaa acgaagtttg tgggctacga cacgctgtcg tctgaagcca
     6901 ccatccaggc gctccttcgg gacggcgcca gagtggatgc gctggcggac ggcgaggaag
     6961 gcgacgttgt gctcgacgtg agcccttttt acgcggaatc gggcggccag atcggcgaca
     7021 caggcaccct ggaatccacc gcgggaagcg ccaacgtgat aacgacccag gtgcccctcg
     7081 aacctctgcg tcttcaccgt gtcaaggtga cgcaagggag cctgagcgtt ggcgacaagg
     7141 tgacggccgc tgtagacggc gcaccccgca tggcgaccgc atgccaccat acggccacac
     7201 atttgctgca ggcggcgttg caggaatccc tgggcgcaca tgtgcgccag gccgggtcgc
     7261 aggtgtctcc ggaacggctc cgctttgatt tcacgcattt tgaagg
//
LOCUS       DAOPBQ010000123         7290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9203_length_7290_cov_4.004008, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000123 DAOPBQ010000000
VERSION     DAOPBQ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9203_length_7290_cov_4.004008"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="PKW60_05540"
     CDS             complement(<1..944)
                     /locus_tag="PKW60_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPJ98928.1"
                     /translation="MKRAAKCIHKKLNEIIEDTLQQVDAEGCERELRALVEGVALALA
                     GETLPAKLSLRAKYERDDAFVRTLCAVQTAIIEALRPELNEDKLREAVLGVRNIFREI
                     TPGLPVSETCRPLRALLEGGPGEYRDDKCVLMWRLLEGVLTNTNDMIYAHDTNGVLFF
                     INASGLEALKYTPEDMRPGLSIYQFVVPDYIDLVEARLESPRAAIRSPYSIEIYTRDG
                     FRIPFEIDTHPLFDDHGDMIAVVGVARSLVLERRLQEAISRANMNLENLFESLPIGVL
                     LANPDGCITNANPMAATLLGARDVNALLGDSAVNLWGG"
     gene            1170..2177
                     /locus_tag="PKW60_05545"
     CDS             1170..2177
                     /locus_tag="PKW60_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ98929.1"
                     /translation="MALNVAVVGMGNIGNVHAECYRKDPLAKLVAVCDIIEERADAAA
                     AKYGCPPFYSVKSMLESGIRIDCASMCTAGKENGGDHYQATMDLLNGGIPVLGEKPIS
                     NEITKARRMVALAKKKRLRYGINLNHRFTPAARLAREWIQRGRLGELNLINMRMWINN
                     PNETSPHFHIRALHPHSIDVMRYFCGDVKRVQAFFKRGKTLNGKQRVCWSNLQLNLLF
                     ANGVLGHLCGSYDAGGGFGLESCEVTGSKGRFVLLEACEDLTFYSRQSTETEHYHHLG
                     GMTGFGETFPDRIHVWLEQNARKTAPAKIDGSNEEALKAQLIIEAAIESWEKGTIVNV
                     R"
     gene            2242..3585
                     /locus_tag="PKW60_05550"
     CDS             2242..3585
                     /locus_tag="PKW60_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPJ98930.1"
                     /translation="MPKRPPNLLIFAIDSIRRDHMSCYGYSRLTTPHMDRLAARGTLF
                     ENAFSAYIPTTPAYVTMLTGRDIVANQQVALSPLGPMADDQPTLPELLKPAGYRSVMV
                     GHGEWYRGFDKYLQYEGWGGPWEERPSRKAENLNAVVLPELERLHASQKPWLLYLRHM
                     DPHSPYYPPPPYDSLFYSKDPCSRKLPDTMKAVRAFKPFADYLLSWMPPGIRDAEFVI
                     AQYDGALAYMDACIQRLVTRTEELGIADNTLIVITGDHGETLYDHECWFDHHGLYEPT
                     LVVPLIFYWPGVINAGVRSQAMTLQEDVVPTLLALMGQKRLARGVRFDGISLKPYFTD
                     TGSSPRSEFYITECTWMRKHGWRTPVWKFWEALEPDFHGKPPVELYNLIEDPQELHNL
                     ADREPELVATLRGRMNNWVRKRCRATGKTNPIELHSIGLDRRIGSIETAQKLQKR"
     gene            3682..4482
                     /locus_tag="PKW60_05555"
     CDS             3682..4482
                     /locus_tag="PKW60_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPJ98931.1"
                     /translation="MITLGVNTVLFQGSDLRTALQHIKWAGFDAAEISAIQGMCEHLN
                     LDNWKSQAGELKALTEDLQLPLTAMEEAALDENRLMQAFEAGAEIGIPVVNVGPGGSK
                     DQPEDLRHCIDLLSKMAEKAEPFGVRLCVKAHVGAAIWNTPTTLDAMRQIDSPAFGID
                     MDPSHIHRGGETPRDALKQVVSRVGHVHIRDCAGRGPSPGPPELQACGRGDIDLMGYC
                     QVLVEAGYDGPMNLEVIGAGDYSLTRCAMIAAESYGYLNACLKACGGR"
     gene            complement(4501..4992)
                     /locus_tag="PKW60_05560"
     CDS             complement(4501..4992)
                     /locus_tag="PKW60_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98932.1"
                     /translation="MPTKYQRQDFREHVAQEHPYLRVARAPGPEVARRLRSPGFMFLF
                     TLSAIIVVLLLGNALSKMAEFRGQPEINGQGVIVSKRLENAGTPSESYVLYVDILREN
                     APAIRREVLADKASFEQFSEGQEVPLVYQMNRTGEDARILTLFMPVSQQEAEPEGMLK
                     RDE"
     gene            5222..6217
                     /locus_tag="PKW60_05565"
     CDS             5222..6217
                     /locus_tag="PKW60_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HPJ98933.1"
                     /translation="MACEAVAYARAGLIGNPSDGYFGKTISFTFRNFAAKVSLYESPQ
                     LEIIPNLRDRSTYNSIQELAEDVRLYGYYGGIRLMKAAISRFAKYCAEHAIDLPARNF
                     AVRYRSNIPRHVGMAGSSALVTATLRCLMEYYEVAIPDTILPNLILSVETDELGIAAG
                     LQDRVIQVYQQCVFMDFDKALMDRQGYGLYERMDPALLPKLFIAYRTDLAEGSEIFHN
                     TIRQRWLRGEPEIVNAMKRFADLAQEAHDLIAAGRGNEIGPLLDANFDLRRSIYTISP
                     ANIDMVERARSAGAHCKFTGSGGAIVGVYEDEEMYAAIEHAFSDTGIVVLKPEIV"
     gene            complement(6259..>7290)
                     /locus_tag="PKW60_05570"
     CDS             complement(6259..>7290)
                     /locus_tag="PKW60_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ98934.1"
                     /translation="SLIHMVGTALVLLQSMSAGAVENAPAGSGALWEARTMAAQARQA
                     LQAAADAGRAGDAETYAAQEAKAEERFAEARRLFEDAGGMQLDDAEALADYARVLSAQ
                     YDADLAADALRKATRLAPENAELWLALGRGLLSPLYPRPEEAKRALTQAVKLGEGGAV
                     EGASRAVLGQYYWNRRLFDFAEEQWVQALDVAPGEFLAQAGLAAVLARRGAVQEASER
                     VAALNQASPDVLASLVQMLGAALNDFAHSGLWFDDTADAHFAYASLLFRAGKYVDCVA
                     PLERTVTLDPKNPVAWNMLGSISMQLGHMERARDAFEASLKLNPEQERTREALKALRA
                     GEQSGDSGP"
BASE COUNT         1517 a         2226 c         2201 g         1346 t
ORIGIN      
        1 tctcctcccc acaggttgac agcggaatcg ccgaggaggg cgttgacatc acgcgcgcca
       61 agcagagtgg ccgccatcgg gttcgcgttc gtgatgcagc catccgggtt ggccagaagc
      121 acgccgatgg gcaggctctc gaaaaggttc tcgagattca tgtttgcccg tgaaatggcc
      181 tcctgaaggc ggcgttccag caccaggctg cgggcgaccc cgaccacggc gatcatgtcg
      241 ccgtgatcgt cgaacagagg gtgggtgtca atctcaaacg ggatacggaa gccgtcgcgc
      301 gtgtaaatct caatggaata gggagagcgg atagctgcgc ggggggattc cagccgcgct
      361 tcaaccagat cgatgtaatc gggcacgacg aattgataga tcgacaggcc gggccgcata
      421 tcttcagggg tatatttcag tgcttccagc ccggatgcgt taatgaaaaa cagcaccccg
      481 ttggtgtcgt gcgcgtagat catgtcgttc gtgttggtga gaacgccttc gagcaggcgc
      541 cacatcagca cgcatttgtc gtcccggtat tcacccgggc ctccttccag gagcgcgcgc
      601 agaggccggc acgtctccga aacaggcagg ccgggggtga tttcacgaaa gatgttgcga
      661 acccccagga ctgcttcgcg caacttgtct tcattcaatt caggccgaag cgcttcaatg
      721 atagccgttt gaaccgcaca caaggtccgc acgaaagcat cgtcccgctc gtacttagcg
      781 cgcagcgaca atttggcggg caaagtctcg ccggccaggg caagggcgac gccttccacc
      841 agcgcgcgga gctcccgttc gcagccctcc gcatcgactt gttgcagggt gtcttcaatg
      901 atctcgttga gcttcttgtg aatgcacttg gcggctcgtt tcatgttccc tccgcccgtg
      961 taaaccccgg tccgcacctc ctgtcgtcaa gcatctccgg gtctctcaag gacttagtat
     1021 aacagagttt gcgggatgtt caaggcttgt ttcaaaagag gattaccgct gctgtttcat
     1081 tcgaactcaa taatttgaca acgaccggtg gagatgcttg aattgccgcc acaaggttct
     1141 ggaaacgcca acagggggag acacttgcaa tggcactgaa tgtggcggtt gtggggatgg
     1201 gcaacatcgg caacgtacat gccgaatgct acagaaaaga cccgctggcc aagctcgtgg
     1261 cggtctgcga catcatcgag gaacgggccg acgccgcagc cgcaaagtat gggtgtccgc
     1321 cgttctacag cgtgaaatcc atgttggaaa gcggcattcg gatcgactgc gccagcatgt
     1381 gcaccgccgg aaaggaaaat ggcggtgacc actaccaggc cacgatggac cttctgaatg
     1441 ggggcatacc cgtcctgggg gaaaaaccca tttcgaatga aatcaccaaa gcgcgccgga
     1501 tggttgcgct ggcgaagaaa aagcgtctgc ggtacgggat taacctcaac caccggttta
     1561 caccggccgc gcgcctggcc cgggaatgga tccagcgggg ccgtctcggc gaactgaacc
     1621 tgatcaatat gcggatgtgg atcaacaacc cgaacgagac gtcgccgcat ttccatatcc
     1681 gggccctgca ccctcactcc atcgatgtga tgcgctattt ttgcggagac gtcaagcgcg
     1741 tgcaggcgtt tttcaaacgg ggcaagacgc ttaacggcaa acagcgggtc tgctggtcga
     1801 acctgcagct gaacctgctg ttcgcaaacg gcgtgttggg ccatctctgc ggcagttacg
     1861 acgccggagg aggattcggt ctggaaagct gcgaggtcac ggggagcaaa ggccggtttg
     1921 tgctcctcga agcctgcgaa gatctgacgt tctacagccg gcaatccacc gaaaccgagc
     1981 actaccatca tctgggcggg atgaccgggt ttggcgagac gtttcctgac cggatccatg
     2041 tgtggctgga gcagaacgcc cggaaaacgg cgccggcaaa aatcgacggc tccaacgaag
     2101 aagccctgaa agcccaactc atcatcgagg cggcaatcga atcgtgggaa aaaggcacca
     2161 ttgtcaacgt ccggtaaaga ccggacggaa cggcaatggc ctcgcctttg ttaaccttca
     2221 acctgcgcaa gaggagctgg catgccaaaa cgaccgccca acctgctgat attcgcgatt
     2281 gacagcatcc gccgcgacca catgagctgc tatgggtatt cacggctcac aacgccgcat
     2341 atggatcgtc tcgcggcccg aggcacgttg tttgaaaacg ccttcagcgc ctacatcccg
     2401 acgacgccgg cgtacgtgac catgctcacc ggccgtgaca tcgtcgccaa ccagcaggtg
     2461 gcgctgtcgc ctctgggccc gatggcggac gaccagccga cgctgccgga attgttgaag
     2521 ccggcggggt accgctcggt catggtcgga catggcgaat ggtaccgcgg atttgataag
     2581 tacctgcagt acgaaggctg gggcgggccc tgggaagagc gcccttcgcg caaggcggag
     2641 aatctgaatg cggtggtcct gccggagctg gaacggctgc acgcctccca aaagccctgg
     2701 ttgctgtatc tccggcacat ggacccccac tcaccctact atcccccgcc gccgtatgat
     2761 tcattgttct actcaaagga tccctgcagc cggaagctgc cggacaccat gaaggccgtc
     2821 cgggctttca agcccttcgc ggactacctg ctgtcgtgga tgccgccggg catccgggat
     2881 gccgagttcg tcatcgccca gtatgacggg gcattggcgt acatggacgc ctgcatccag
     2941 cggctcgtga cgcgcacgga agaactgggc atcgccgaca acaccctgat cgtcatcacg
     3001 ggagaccacg gggagacgtt gtacgaccac gaatgctggt ttgatcacca tggactctat
     3061 gagccgacgc tggtggtgcc cctgatcttc tattggccgg gcgtaatcaa cgcgggtgtc
     3121 cgcagccagg caatgacgct ccaggaagac gtggtgccga cattgctggc cctcatggga
     3181 cagaaacgcc tggcccgcgg ggtcaggttc gacggaatat cgctcaaacc ttattttacg
     3241 gacaccggca gttcaccacg ctcggaattc tacatcaccg agtgcacgtg gatgcgtaaa
     3301 cacgggtggc ggacgcccgt ctggaagttc tgggaagcgc tggagccgga tttccacggc
     3361 aaaccgccgg tggagctcta caatctgatc gaggacccgc aggaactgca taacctcgcg
     3421 gatcgcgagc cggaactcgt ggcaacctta cgcggccgta tgaacaactg ggtccggaaa
     3481 cggtgccgcg ccacgggcaa gaccaacccc attgaattgc acagcatcgg cctcgaccgg
     3541 cggatcgggt ccatcgaaac cgcccagaaa ctgcagaagc ggtagtggcg ccggttcatc
     3601 caggcagcgg ccggcccggg ccggcatgaa acgggcgttc ggcacggtaa acgccggccc
     3661 caaagacgag aggatgcaag catgatcaca ctgggcgtga acacggtctt gtttcagggg
     3721 agcgatctgc gtacggccct gcagcacatc aaatgggccg gattcgacgc agcagagatc
     3781 tcggcgattc aagggatgtg cgaacatctg aacctggaca actggaagag ccaggcaggc
     3841 gaactcaagg cgctcacgga agatttgcag cttcccctca ccgccatgga agaagctgcc
     3901 ctcgacgaaa accggctcat gcaggccttt gaagcaggtg cggagatcgg gatcccggtc
     3961 gtgaacgtcg ggcccggcgg ttcaaaggat cagccggaag acctccgcca ctgcatcgac
     4021 ttgctgtcga aaatggcgga aaaggcggag cccttcgggg tgcgtctgtg cgtgaaagcc
     4081 cacgtagggg cggcgatctg gaacacaccc accacgctgg acgccatgcg gcagatcgac
     4141 tctccggcct tcggcatcga tatggatccg agccacatcc accggggagg cgagacgccc
     4201 cgggatgcgt tgaaacaagt cgtgtcgcgg gtagggcatg tgcatatccg tgactgcgcg
     4261 gggcggggcc cctcccccgg gccgccggaa ctgcaggcct gcgggcgcgg cgatattgac
     4321 ctcatgggct actgccaggt cctcgttgaa gcggggtatg acggccccat gaacctcgaa
     4381 gtgatcggcg cgggcgatta cagtctcacc cgctgcgcga tgattgcggc ggaatcgtat
     4441 ggatacctga acgcatgtct gaaggcctgc ggcggccgtt aaaggcccgg cccgacggca
     4501 ctattcgtcc cgtttcaaca tgccctcggg ttcggcttcc tgttgggaga ccggcatgaa
     4561 aagcgtaagg atccgggcgt cttcaccggt tcggttcatc tgataaacca ggggcacttc
     4621 ctggccctcg gaaaactgtt caaaactggc tttatccgcc aggacttcgc ggcgaatcgc
     4681 gggggcgttt tcacggagga tatccacata gagcacgtag ctctctgacg gagtgccggc
     4741 attttcgagc cgtttcgata cgatgacgcc ctggccgttg atctcgggct gcccgcggaa
     4801 ctccgccatt tttgacaggg cattcccgag cagcaacaca acgatgatgg cgctgagcgt
     4861 aaacagaaac atgaatccag gcgagcgcag ccggcgcgct acctccgggc cgggcgctct
     4921 cgcaacgcgc aggtacggat gctcctgagc cacatgctcc cggaaatcct gccgctggta
     4981 ttttgtaggc attctgtgca ctctccagga ctctacagcc aagctccgca ttcggcgtcc
     5041 tgaccaaccc tgcgcctatt gtagcactct ttgcagtggc aggatcccct ggagacgggg
     5101 ttcggcacgc ccctgggacc catgacgctg aaccaggaca tagcgcggat aacgaggcgc
     5161 cgcattgaac cagggcgcgt gcttgcgtat cctcctggat attccagatg aggaggcctg
     5221 tatggcgtgt gaagcagttg cgtatgcccg ggcaggcttg atcggaaatc cgtcggacgg
     5281 gtatttcgga aagaccatca gtttcacgtt ccgcaatttc gcggccaagg tgagcctgta
     5341 cgaaagcccg caactcgaga tcattccgaa cctgcgcgac cggtccacct acaattcgat
     5401 ccaggagctc gccgaagacg tacgcctgta cggctactac ggcgggattc gcctcatgaa
     5461 ggcggccatc agccgctttg ccaaatattg cgccgaacac gcgatcgacc tgccggcacg
     5521 gaacttcgcg gtgcggtacc gctcgaacat cccgcgacac gtggggatgg caggttccag
     5581 cgccttggtc acggcgaccc tgcgctgcct gatggaatat tacgaggtgg cgatccccga
     5641 tacgattctg cccaatctta ttttgagcgt cgaaaccgat gagctgggga tcgcggccgg
     5701 gctgcaggat cgcgtcatcc aggtctatca gcaatgcgtt ttcatggatt tcgacaaggc
     5761 cctgatggac cgtcagggat acgggctgta cgaacgaatg gatccggccc tgttaccgaa
     5821 actcttcatc gcataccgca ccgatctggc ggaaggctcg gagatttttc ataacaccat
     5881 ccgccagcgc tggctgcgcg gtgagcctga aattgtaaat gccatgaagc gtttcgcgga
     5941 tttggcccag gaagcgcacg acctgatcgc cgcaggacgc ggaaatgaga tcggccccct
     6001 gctggacgcc aactttgatc tgcggcgttc catctacacg atcagccccg cgaatattga
     6061 catggtggag cgggcgcgtt ccgccggggc gcactgtaag ttcacgggtt ccggcggggc
     6121 cattgtgggg gtgtacgaag atgaggagat gtacgccgcc atcgaacacg cattcagtga
     6181 caccggcatc gtggtgctta aacccgagat cgtgtagctg ccaggcagat gccccggaat
     6241 gggcaggggc aattgggatc agggcccgct gtccccgctt tgctcaccgg ctctcagggc
     6301 cttcagtgcc tcccgcgtgc gttcctgctc gggattcagc ttcagcgacg cctcaaaggc
     6361 gtcccgggca cgctccatat gccccagctg catgctgatc gagccaagca tgttccacgc
     6421 cacgggattt ttcggatcga gcgtgaccgt gcgctcaagc ggcgccacgc agtccacata
     6481 cttgcccgct cgaaacagca gcgacgcgta cgcgaaatgt gcgtctgctg tgtcgtcaaa
     6541 ccagaggccg gaatgcgcaa aatcattcag agccgcgccc agcatctgca caagtgaagc
     6601 gagcacgtcc ggagaggcct ggttcaacgc ggcgacgcgc tccgatgcct cctgcacggc
     6661 gccacgccgt gctaaaacag cagcaaggcc tgcctgggcg aggaattctc caggcgcaac
     6721 atccagagct tgaacccact gctcctccgc gaaatcgaac agtctcctgt tccagtaata
     6781 ctggcccagg accgcacgcg acgcaccctc gacagctcct ccttcgccca gcttcaccgc
     6841 ctgggtcaag gcgcgttttg cttcctcggg gcgcgggtac aacggcgaca aaagcccccg
     6901 cccgagtgcc agccacaatt ccgcgttttc cggcgccagg cgcgtggctt tgcgaagcgc
     6961 atccgccgcg agatccgcat cgtattgcgc agacagcacc cgtgcgtaat cggccagcgc
     7021 ttccgcatcg tcgagctgca taccgcccgc gtcttcaaac aggcggcgcg cctccgcgaa
     7081 cctctcctcc gccttggcct cttgcgctgc ataggtttca gcatcaccgg cgcggcctgc
     7141 gtcggccgcc gcctgaagcg cctgacgggc ctgcgccgcc atcgtgcgcg cttcccagag
     7201 ggcgcccgaa cctgccggcg cattttccac ggccccggca ctcatagact ggagcagcac
     7261 caaagctgtc cccaccatgt gaataagaga
//
LOCUS       DAOPBQ010000124         7281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9210_length_7281_cov_4.019374, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000124 DAOPBQ010000000
VERSION     DAOPBQ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9210_length_7281_cov_4.019374"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKW60_05575"
     CDS             complement(<1..331)
                     /locus_tag="PKW60_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPJ98935.1"
                     /translation="MAEKVNKIVLAYSGGLDTSVILKWLQEIYKADVIAFIADIGQGE
                     ETAPAVAKAKATGASGVYCEDLREEFVRDFVFPAMRANALYEGCYLLGTSVARPQPSY
                     RALARMAG"
     gene            complement(366..1325)
                     /gene="argF"
                     /locus_tag="PKW60_05580"
     CDS             complement(366..1325)
                     /gene="argF"
                     /locus_tag="PKW60_05580"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPJ98936.1"
                     /translation="MYTDFLSLADLSADEILELLELADRLKAELKAGKREAPLAGQTL
                     AMIFEKPSLRTRVTFEAGMVQLGGHALLLEMLTTRLGERESVPDVARNLARWVDGIMA
                     RTYSHDTVLQLAEYAQIPVINALTDKLHPCQVLADAQALREHKGGFQDLKLAFVGDGN
                     NVCASWFNFAARLPLNVTLVCPPGYELEPAVVEFAQREARGEVKITYDLEEGLAGADA
                     VYTDVWASMGQESESASRAKIFAPYQVNARIMKLANPGAVFMHCLPAHRGQEVTDDVI
                     DSPQSIVLDQAENRLHAQKAVMLTLMRKDAAGLNRKQRQPARG"
     gene            complement(1325..2512)
                     /locus_tag="PKW60_05585"
     CDS             complement(1325..2512)
                     /locus_tag="PKW60_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPJ98937.1"
                     /translation="MNTQEIKTLNDKYIINTYGERKLAFVRGLGSRLWDADGNEYLDF
                     FAGIAVVSLGHCHPAVTEAICNQARTLVHTSNLYYIEPQVQLAERLAAHSFADRWFFA
                     NCGATANETAIKLARRYWAQQGTPKPHIITAVNSFHGRTLATITATGQEKYQAGFEPM
                     MPGFSYAPYDDVAALEAAITPETGAILLEPVQGEGGVRVPSDGYLPAVRRLCDERGIL
                     LLFDEVQTGMGRTGKLFCYEHSGITPDIVTLAKALGNGVPISAMGCTEKVASGFEPGM
                     HATTFGGNPLCCAAALATLNVLLANGFLQAVRETGAYLMNALSGLASKHAIIKDVRGK
                     GLMVGVELDQPAGPVVGRMINAGIVCGPAGPNVLRFLPPLIVTRNDVDRVVSTLDAVL
                     GEA"
     gene            complement(2695..3021)
                     /locus_tag="PKW60_05590"
     CDS             complement(2695..3021)
                     /locus_tag="PKW60_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPJ98938.1"
                     /translation="MPTYTYECPKCQHTLDLFHAMTKSPRVKCPACGHAQMKRLLGSG
                     AGIIFKGSGFYETDYKNNRSKGKAADDMAAKSESPAKDSSPAKTKDSGKSEPSGKKKS
                     GGGKAA"
     gene            complement(3225..4322)
                     /locus_tag="PKW60_05595"
     CDS             complement(3225..4322)
                     /locus_tag="PKW60_05595"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPJ98939.1"
                     /translation="MTESTRPPAAASSAGCGQENYHSHVLDALASGVLALDLDGRVIA
                     ANEAACAHLGIDPSVLAPGAQLQTVPRLEIMAPFFEQVLKSKELLERQELLLDTGEED
                     RKEIGLSAAPLMIDDNIRGVVFLFTDMTVRRKFERAAEINRQLAALGELTAGVVHELR
                     NPMSVISAMAEILMRKLAESDERYAAAETIFREAGSVEKAISQFLGFARPYDVHPVHC
                     TPGEIADRAIQLCSRRAEKYGVTLQCEILANLPSMQADVGRAAQALANIINNGIDAAG
                     ENGRVSLTVRREGNEIVFEIIDTGEGIQLKSGEDVFKPFFTLKEGGTGLGLAIVHRII
                     TAHNGSVRHGNRPEGGACFEVRLPVERGALR"
     gene            complement(4345..5211)
                     /gene="rsmA"
                     /locus_tag="PKW60_05600"
     CDS             complement(4345..5211)
                     /gene="rsmA"
                     /locus_tag="PKW60_05600"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPJ98940.1"
                     /translation="MHLQDLCSRYNIRFKRQLGQNILVDDNINRIMVDAAGLTRDDDV
                     IEVGAGLGALTQHLHPVARRVLAIEIDFSFMPCLQDRFGEIDNVLLFRGDVLNHGVDK
                     LAGEFLPGGERYHLLSNLPYYITTPVLFHFWESPLPMRQMVVMVQEEVALRLTATVDT
                     PDYGMLAMAARYYGGVDLIRRVPRACFRPIPKVDSCVVRLRAHETGLYPDVERSFLMA
                     LMRAAFGQRRKTLRNSLTKSGSFGAPREAVLEAMEAADIEPGRRPQTVTLDEFVRLAR
                     EIRARIHQGLSS"
     gene            5340..6980
                     /locus_tag="PKW60_05605"
     CDS             5340..6980
                     /locus_tag="PKW60_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPJ98941.1"
                     /translation="MAELYIEQPGLTSFTVPLEGQALRLGRAEDNDVVLQASEVSRHH
                     AVLEYRAGVFLLTDLKSMNGTYVNRQRIVERALSDQDDIWLGSKCHIIFHDPDGRAEF
                     DLREPRGETSSIIQDLDVIRAEMDRIGDSMTLIGKRGLSPTDRTPAMKEDGEANLIGI
                     GRAYRRLAALYEASKLMASEFDLDKRLTDMLDKVMDVMEAERGFVMMHDPRAGRLAVR
                     VARQMGYELEAGSPSMGIAEKAARDGEPVLMSSREKDLQFGGRASVIQQAITSAMCAP
                     LKTGGRVLGSIYLDTSLPGAVFSSEDLELFASLAAQSAMAIENVRLYREAVEAEKTRA
                     NLGRFLSPAIVEQVMKHDTALQLGGDKRVVTVMFCDIRSFTAIAEQTAPIDLVTLLNQ
                     HFTAMAEIIFQHAGTLDKFIGDEVMAVFGAPLAAEDDAERCVRAAMAMQSKNEELNAL
                     RRTAGQPEFEVGIGINTGETIAGYVGSPDRMEFTVVGDSVNVARRFCALAAPGQVVIG
                     EATFEMVEHAFETHPIGSVLLKGKSSTKHGYEVLRPKS"
     gene            complement(7095..>7281)
                     /locus_tag="PKW60_05610"
     CDS             complement(7095..>7281)
                     /locus_tag="PKW60_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98942.1"
                     /translation="QPDGTPIPGFTLDDCDELIGDQIDRVVTWKGSGDVSALVGTPIR
                     LRFVMKDADLYALRFRP"
BASE COUNT         1330 a         2215 c         2299 g         1437 t
ORIGIN      
        1 tccccgccat gcgcgccaac gccctgtatg aaggttgcgg cctcgcgacg ctggtgccga
       61 ggaggtagca accttcatac agggcgttgg cgcgcatggc ggggaacaca aaatcgcgca
      121 cgaattcctc gcgcagatcc tcgcaataga cgcccgaggc gccggtggct ttggctttcg
      181 cgacggccgg ggcggtctct tccccctggc cgatgtcggc gatgaaggcg atcacgtccg
      241 ccttgtagat ttcctggagc catttcagga tgaccgaggt atcgagtccg cctgaataag
      301 cgagcacgat tttgtttacc ttctccgcca tgcgatgctc tccttgctgt cgcgcgccgc
      361 ggtgcttatc cgcgggcggg ttgtctctgc ttacgattga ggccggccgc atccttacgc
      421 atgagggtca gcattaccgc tttctgggcg tggaggcggt tttcggcctg atcgaggaca
      481 atggactgcg ggctgtctat cacgtcatcc gtaacttcct gaccacggtg tgcgggcagg
      541 caatgcatga aaacggcgcc ggggttggcg agtttcatga tgcgcgcatt gacttgatac
      601 ggcgcaaaga ttttggcccg gctggcgctt tcactctctt gccccatgct tgcccagacg
      661 tcggtataga cggcatccgc gccggcgagg ccttcctcga ggtcgtaggt gatcttcacc
      721 tcgccgcggg cctcacgctg ggcaaattca accaccgcgg gctcaagctc gtatccgggc
      781 gggcacacca gcgtcacgtt cagggggagc cgggcggcga aattgaacca ggaggcacag
      841 acgttgttgc cgtcgcccac aaacgcgagc ttgaggtcct ggaagccgcc cttgtgttcg
      901 cgcagggcct gtgcgtcggc caggacctgg cagggatgca gtttatcggt cagggcattg
      961 atcacgggga tctgcgcgta ttcagcgagt tggagcaccg tatcgtggct gtaggttcgc
     1021 gccatgatgc cgtcgaccca gcgcgcgaga ttacgcgcaa cgtccggcac cgactcacgc
     1081 tcgccgagcc gcgtggtgag catttcgagc aggagggcat ggccgcccag ctgaaccatg
     1141 cccgcctcaa acgtgacgcg cgtgcgaagg ctcggtttct cgaaaatcat ggcgagggtc
     1201 tgtcccgcaa gcggggcctc gcgttttccg gctttgagct cggctttcag ccgatcggcc
     1261 agttcgagga gctcgagaat ctcgtccgcc gacaggtcgg ccagagacag aaagtcggtg
     1321 tacatcaggc ttctcccagt acggcatcca gggtggagac gacacgatcg acatcattcc
     1381 gggtgacgat gagcggcggc aggaagcgca gaacgttggg tcccgcgggc ccgcacacga
     1441 tccccgcgtt gatcatgcgc cccacaacgg ggccagcggg ctggtccaat tcgacgccga
     1501 ccatgaggcc tttgccgcgc acatctttga tgatggcgtg tttggaagcc agcccggaga
     1561 gcgcgttcat gagataggcg ccggtctctc gcacggcctg cagaaaaccg ttggcgagca
     1621 ggacattcag ggtggcgagg gccgccgcgc aacacagggg atttccgccg aaggtcgtgg
     1681 catgcatccc cggctcaaac ccggaagcca ccttctccgt gcatcccatg gcgctgatgg
     1741 ggaccccatt gcccagggct ttggccagag tcacgatatc cggcgtgatg cccgaatgct
     1801 cgtagcagaa gagctttccg gtacgtccca tgccggtctg gacttcgtca aagagcaaca
     1861 ggatgccccg ttcgtcgcag aggcggcgca cggcgggcag gtagccgtca ctggggacgc
     1921 gcacaccgcc ctcgccctgg acgggttcga gcaggatggc gccggtttca ggcgtgatgg
     1981 cggcttcgag ggcggcgacg tcgtcgtagg gggcgtagct gaatcccggc atcataggct
     2041 cgaatccggc ctggtatttt tcctggccgg tggcggtgat ggttgcgagc gtgcgcccat
     2101 ggaacgaatt tacggcggtg atgatgtgag gtttcggcgt tccctgttgc gcccagtagc
     2161 gccgcgccag tttgatggcc gtttcattgg ccgtggcgcc gcaattcgca aagaaccagc
     2221 ggtcagcaaa cgagtgtgcg gccaggcgct ccgccagctg gacctgcggc tcgatgtagt
     2281 agaggttgga ggtgtggacc agcgtgcgcg cctggttaca gatcgcctct gtcacggcgg
     2341 gatggcagtg ccccaggctg accacggcga tcccggcgaa gaaatcgagg tattcgttgc
     2401 cgtcggcgtc ccagagacgg ctgcccagcc ctcgaacgaa ggccagcttc cgttccccgt
     2461 acgtgttgat gatgtatttg tcgttcagcg ttttgatttc ctgggtattc actggttcaa
     2521 cctttcttcg ctggcgtttc gagggcgccc gcggcaaaaa cagaaaagcc ctcccacgga
     2581 ggcgcgtatt cacccacgct gctctggcgt gacggagtcc cgcggctcac aggcgccggc
     2641 ggctggtgca tgaccgtatg caatatcacc gtgtatgcag agtttcaatg gtgctcaggc
     2701 ggctttcccg ccccccgatt ttttcttgcc ggaaggctcc gatttcccgc tgtctttggt
     2761 tttggcgggg ctgctgtctt tggccgggct ttcgctcttc gcagccatgt cgtcggccgc
     2821 tttgcccttg ctgcggttat ttttgtaatc ggtttcgtag aaaccggagc ccttgaagat
     2881 gatccccgcg ccggatccca ggagccgttt catctgagca tgaccacaag cgggacattt
     2941 cacgcgcggc gatttcgtca tcgcgtgaaa caggtccagg gtatgttgac atttcgggca
     3001 ttcgtacgtg taggtgggca tggataccga tactcctgtg cgtctgaccg tcgttggggc
     3061 tgtccatgca gtctccgatt ttgggacatg cacggtgaac cctctgcgag aaaccggtac
     3121 tttagcacgg acagaacgtc aatgcaacgt tacgacggca ataaacccaa gcaaatgcaa
     3181 cgagttagag gtgcttgagc ccgggaggcc gctggcgccg ggattcagcg cagggcgcct
     3241 cgttccacag gcagccggac ctcaaaacac gcgccaccct cgggccggtt gccatggcgt
     3301 accgagccat tgtgcgcggt aatgatccgg tgcacgatgg ccagccccag gccggtcccc
     3361 ccctccttga gcgtgaagaa cggtttaaaa acgtcttcgc ccgatttgag ctgtatgcct
     3421 tcgcctgtgt cgatgatctc gaacacgatc tcatttcctt cgcgcctcac ggtcagggag
     3481 acgcggccgt tttcgccggc ggcatcgatt ccgttgttga tgatgttggc cagggcctgg
     3541 gcggctctgc ctacgtccgc ctgcatggaa ggcaggttcg ccaggatctc gcattggagt
     3601 gtgacgccgt acttttcagc gcgccgggaa cacagctgga ttgcccggtc agcgatctct
     3661 ccaggggtgc aatgcacggg atgcacatcg taggggcggg cgaagcccag gaattgcgag
     3721 attgcctttt cgacgctgcc cgcctcccga aaaatggtct cagcggcggc ataccgttcg
     3781 tcgctctcgg ccagtttgcg catgaggatc tccgccatgg cgctgatcac gctcatggga
     3841 ttgcgcagct catgcacaac acccgcggtg agctcgccca gagcggcgag ctggcggttg
     3901 atctcggccg cgcgttcaaa cttgcgccgc accgtcatgt ccgtgaacaa aaacacgaca
     3961 ccgcgaatgt tatcgtcaat catcagcggc gccgcggaga gtccgatttc tttgcggtct
     4021 tcttccccgg tatcgaggag aagttcctgg cgttccagga gttctttcga tttcagcact
     4081 tgttcaaaga agggcgccat gatttcgagt ctgggcacgg tttgcagctg ggcgcccggc
     4141 gcgagcacgc tgggatcgat gccgaggtgc gcgcaggccg cttcgtttgc cgcgataacc
     4201 cgtccgtcga gatcgagcgc caagaccccg ctggccagag catccaggac atgagaatgg
     4261 taattttcct gcccgcaccc ggcgctggat gcagccgccg gtggacgcgt tgactccgtc
     4321 atggacgcca tactccttta ggtttcagct tgaaagcccc tggtggatcc gggctcgaat
     4381 ctccctggcc aggcgcacga attcgtccag cgtcacggtc tgtggcctgc ggccgggctc
     4441 gatgtctgcg gcctccatcg cttccagcac tgcttcgcgg ggcgctccaa aactcccgga
     4501 tttcgtcagt gaattgcgca gtgttttccg gcgctgcccg aaagcggccc gcatcagggc
     4561 catcaggaag gagcgctcca cgtcaggata caggcccgtt tcgtgtgcgc gcaaacggac
     4621 tacacaactg tctaccttgg gtatgggccg gaaacacgcg cggggcacgc gccggatcag
     4681 gtccacgccg ccgtaatacc gggcggccat ggcgagcatg ccgtaatcgg gtgtgtcgac
     4741 ggttgcggtt aagcgcaagg ccacttcttc ctgcaccatg accaccatct ggcgcatggg
     4801 caggggcgat tcccagaaat gaaagagcac cggcgttgtg atgtaatagg gcaagttcga
     4861 cagcaaatgg taccgctcgc cgcccggaag aaactcgccg gccagtttgt ctaccccatg
     4921 gttcagcaca tcgccccgga acagcagcac gttgtcaatc tctccgaaac ggtcctgaag
     4981 gcagggcatg aacgagaaat cgatctcaat ggccagcacg cgccgtgcaa ccgggtgcag
     5041 gtgctgcgtg agagcgccca ggcccgcccc cacctcgatg acgtcgtcgt cgcgggtcag
     5101 gcccgcggca tcgaccatga tgcggttgat gttgtcgtca acgaggatat tctggcccag
     5161 ttggcgctta aaacggatat tgtacctgct gcacaggtcc tgtaaatgca taccacccct
     5221 ccttttgccg ggcgccctgc cggcatgaag ccagccactg cgccgcccgg ggcgcgtgcc
     5281 gcccgttgac cctccgcgac gggattgcta tcatcaaagc ctagcacggg atatgggcca
     5341 tggcggaatt gtacatcgaa caacccggcc tcacatcttt cactgtgcct ttggaaggcc
     5401 aggcccttcg ccttggccgc gccgaagaca acgatgttgt cctgcaggcg agtgaagttt
     5461 cacgccatca tgccgtactc gaataccgtg cgggcgtatt ccttctgact gacctcaaaa
     5521 gcatgaacgg cacctacgtg aaccgccagc gcattgtcga acgggcgctg tcggatcagg
     5581 acgacatctg gctcggcagc aaatgccaca tcattttcca cgacccggac ggccgcgccg
     5641 aattcgacct gcgtgagccg cgcggtgaaa cctcctcgat catacaggat ctcgacgtaa
     5701 ttcgcgccga aatggatcgc atcggcgaca gcatgacctt gataggcaag cgcggcctgt
     5761 cgccgaccga tcggacgccg gccatgaagg aggatggcga ggcgaatctc attggcatcg
     5821 ggcgcgcgta ccggcgtctg gccgcattgt acgaagccag caagctgatg gcctcagaat
     5881 tcgacctgga caaacgcttg acggacatgc tcgacaaggt catggatgtc atggaggccg
     5941 agcggggctt cgtcatgatg cacgaccccc gggccggccg cctggcggtc cgggtggccc
     6001 gccagatggg ttacgagctg gaagccggtt cacccagcat gggcattgcc gagaaagccg
     6061 ctcgcgacgg agaacccgtg ctgatgtcca gccgtgagaa ggaccttcag ttcggaggcc
     6121 gcgccagcgt catccagcag gcgatcacca gcgccatgtg cgcaccgctc aagacggggg
     6181 gccgtgtact cgggtccatt tatctcgaca ccagccttcc aggggcggtg ttctccagtg
     6241 aagaccttga actgttcgcc tcgctcgcgg cgcagtcggc catggccatc gaaaatgtcc
     6301 ggctataccg cgaggcggtg gaggcggaga agacccgcgc caatctgggc cgtttcctgt
     6361 cgccggcgat tgtggagcag gtcatgaaac acgacaccgc gctgcagctt ggcggggaca
     6421 agcgtgttgt gacggtgatg ttttgcgaca tccgctcctt cacggcgatc gcggaacaga
     6481 cggcgcccat tgatttggtc acgctgctga accagcattt caccgcgatg gccgagatca
     6541 ttttccagca cgcgggcaca ctcgacaaat tcatcggcga tgaagtgatg gcggtgttcg
     6601 gcgctccgct cgccgcggag gacgatgccg aacgctgcgt gcgcgcggcg atggcgatgc
     6661 agtcgaagaa tgaagagctt aacgcgctcc ggcggacggc aggccagccc gaatttgaag
     6721 tgggaatcgg catcaatacg ggagagacca ttgcgggcta tgtcggctct cccgaccgaa
     6781 tggaattcac ggtcgtcggc gacagcgtca acgtggcgcg ccggttctgc gctcttgccg
     6841 ccccaggcca ggttgtaatc ggtgaggcca cgtttgagat ggtcgaacac gcattcgaga
     6901 cccatcccat cggctcggtg ctgctgaaag ggaaatcttc gacgaagcac ggctacgagg
     6961 tgctgcgccc gaaatcgtga ccggtcgcgg cccgcagcac cttccacgtc accgctgacc
     7021 ggtaccgggc ggcctatccg ccgtatggcc gccggcattc cgctgttatt tcaattagag
     7081 tggcgcatac aggatcacgg ccggaatcga agcgcgtaca ggtcggcatc cttcatcaca
     7141 aagcggagac ggatgggcgt gccgacgagg gcgctgacgt cgccgctgcc tttccaggtc
     7201 accacgcggt cgatctggtc gccgatgagc tcgtcgcagt catccagcgt gaagcccggg
     7261 atcggcgtgc cgtcgggttg c
//
LOCUS       DAOPBQ010000125         7255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9253_length_7255_cov_3.947500, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000125 DAOPBQ010000000
VERSION     DAOPBQ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9253_length_7255_cov_3.947500"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /gene="cobA"
                     /locus_tag="PKW60_05615"
     CDS             complement(<1..472)
                     /gene="cobA"
                     /locus_tag="PKW60_05615"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HPJ98943.1"
                     /translation="MTRQFGTVYLVGAGPGDPGLITVRGRECIARADVIIYDALANPQ
                     LLVHARPGAECIFAGKERGRHTLIQEEINRLLVEWAGKADHVVRLKGGDPFVFGRGGE
                     EAQLLARHGIPFEIVPGITAGIAAPAYAGIPVTHRGVAASATLLAGHSPGNEPGK"
     gene            complement(469..1389)
                     /gene="hemC"
                     /locus_tag="PKW60_05620"
     CDS             complement(469..1389)
                     /gene="hemC"
                     /locus_tag="PKW60_05620"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPJ98944.1"
                     /translation="MPERIVIGSRGSELALCQSELIASGLRALEPGTRVDIQVIRTSG
                     DKITDVPLAQIGGKGLFTKEIEEALLAGDIDLAVHSMKVLPTELPEGLCIGAVPKRES
                     PCDVLVSAGNLTLEQLPPGARVGTSSLRRTAQLRAYRSDLEILPLRGNVTTRLRKVSE
                     GSVDAAILAAAGLSRLGLSDRVSQEIPVTLMLPAPAQGALAIEARVDDTKILRLLAPL
                     DDPETRSAVAAERIFLAAMEGGCQVPLGALASRSGEQLVLDACVCSLDGARVLRCRTA
                     GRHDRPEEAGNQAAVELRNLGASALLESLR"
     gene            complement(1413..2660)
                     /gene="hemA"
                     /locus_tag="PKW60_05625"
                     /pseudo
     CDS             complement(1413..2660)
                     /gene="hemA"
                     /locus_tag="PKW60_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
     gene            complement(2648..3502)
                     /gene="ccsA"
                     /locus_tag="PKW60_05630"
     CDS             complement(2648..3502)
                     /gene="ccsA"
                     /locus_tag="PKW60_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPJ98945.1"
                     /translation="MTTLTLAVLFAGTLAFAGATAAAVLYLANKREAALNLTRRLALL
                     GAGLFLLALLLRGAAWHILPMTTAADALIVFVIFATIVMFVSARDSTRAVASFYTPAL
                     FVLAVLGASLAPATFGHAPKELRGLFLAVHVGLAFLAYAFFLGASVASVAYLFQSRHL
                     KKHRTVGLFQALPPLERLDKSLYRLIYHGYVMFAATLLTGAIWARVDRALLGPHWFLA
                     PKVLQSVVMFLFYAFVFHGRQCGLLRGRKLAFAVLIGFSAMLGIYLARTVLNLSYYYF
                     WEPSTWAS"
     gene            complement(3499..4137)
                     /locus_tag="PKW60_05635"
     CDS             complement(3499..4137)
                     /locus_tag="PKW60_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HPJ98946.1"
                     /translation="MSLYLISLKIAERPCLVTGGGHIALPKARQLVEAGARVTVVSPQ
                     FAPGFAELSATLVQREFEPGDLDGMFLAIAATDDPEANRSVGEICQARGILCNVVDDP
                     EVCGFYLNSVLRRGDLQIAVSTGGAAPALAKRLREDLEPLFPADYGEYLDFLRQARGQ
                     ARSRVAVPALRARIAYELASEAGYQRFRQLDAAQRSGWVEQVIAQNQPETTA"
     gene            4463..5350
                     /locus_tag="PKW60_05640"
     CDS             4463..5350
                     /locus_tag="PKW60_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPJ98947.1"
                     /translation="MYLSIRDAMVSGDEFPSPVAGLRHLAVENVEVRMTPDYQVCAMD
                     SKEMITLASDEDAKAYKKHLDGLGIRACCFMTAKDFSTGDNESNAAWVARAIELADIM
                     EMAVVRIDSAMSKERELDFEKRVQLFAEGLGGALERTAGSKVALGIENHGFQGNNLAF
                     QLNVYQSVGSDRLGATMDTGNFYWRGYPLSEVYGILRILAPYTKHTHAKNINYPEDQR
                     EITREPGWEYARYCCPLDEGDINHAKVLRMLADAGYDGDVCIENESVGRYKTPGERIE
                     VLERDVAHCRAIIQDLKLR"
     gene            5368..5976
                     /locus_tag="PKW60_05645"
     CDS             5368..5976
                     /locus_tag="PKW60_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98948.1"
                     /translation="MGDEQPKTAKPPWRGIAWLVSGLILALGASFLAQRFDLGRRFAL
                     DFKGKHVIGIAERMGDDYNIRYEHPDGTIFRRLYKGTLSGSPSSDGKFEIAIVFDPGE
                     PSQFQPAGLSYLPGVAALTLFILGMASVFKGRKVMYAHYLAATGKPEVGKAGKRGAGP
                     GRDPAQARRKQLRIALTLALAFALIVLIYGCPQFNIPGLFKN"
     gene            complement(6000..>7255)
                     /locus_tag="PKW60_05650"
     CDS             complement(6000..>7255)
                     /locus_tag="PKW60_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPJ98949.1"
                     /translation="ALAGAGAIGGYPDLASCRQGLMALSQQDPVVDRYLSEAESLRAR
                     AAELVGSRRFPEALETAGQIQRAVLQGYCAAQKPLPGEHRAYWCHSAYGVDGVTWDEA
                     IRRLADNGFTAIMPNMVWGGVAFYPSDVLPAAAELAQKGDQVAQCLAACRKYGIECHV
                     WKVCWNMGTRAPKSFMDRMKSEGRTQVSFAGVAQPQWLCPSHPANQQLEIDSLVELAR
                     KYDVDGIHFDYIRYPGSEYCFCAGCRARFEQQLGRPVAHWPKDVGSGGRDLDAWLEFR
                     RQNITKVVAGVHDGARKARPGIQVSAAVFRSWPHDRDKVGQDWKLWCDRGYLDFVCPM
                     DYTPHNDSFRNMAAQQLAWTGNVPCYPGIGQSTWPDRLDAVKLIEQISITRELGTGGF
                     TVFNYGTAEYADLLPLCGMGITRKP"
BASE COUNT         1356 a         2225 c         2396 g         1278 t
ORIGIN      
        1 gttttcccgg ctcgtttccg gggctgtgcc cggcgagcag ggtggcgctg gcggccacgc
       61 cacggtgggt gaccgggatc cctgcatacg cgggggcggc aatccctgcg gtgatgccgg
      121 gcacaatttc gaaggggatc ccgtgccggg ccaaaagctg ggcttcttcg ccgccgcggc
      181 caaagacaaa agggtcgccg cctttgaggc gcacgacatg gtcggctttc ccggcccatt
      241 cgaccagaag ccggttgatc tcttcctgaa tgagcgtgtg gcggccgcgt tccttgccgg
      301 cgaagatgca ctccgcgccg ggacgggcgt gcacgagcag ctgggggttg gcgagagcat
      361 cgtagatgat gacgtcggcg cgcgcgatac attcacgccc gcggaccgtg atgagtccag
      421 gatccccggg ccccgcgcca acaagataga ccgtgccgaa ttgccgggtc atcgcagcga
      481 ctccagcagg gcgctggcac ccaggttgcg cagctccaca gcggcctggt tgccggcttc
      541 ttcggggcga tcatgcctgc cggccgttct gcagcgcaag acccgggcgc catcgaggct
      601 gcacacgcag gcgtcaagca cgagctgctc gccggaacgg gacgccaggg cgccgagagg
      661 aacctggcac ccgccctcca tcgccgcaag aaagatgcgc tcggcggcaa cggcgctgcg
      721 ggtctccggg tcgtcgagcg gcgcaagcag tcgcaggatc ttggtgtcat ccacgcgtgc
      781 ttcaatggcg agcgcgccct gagcgggcgc gggcaacatg agcgtcacgg ggatttcctg
      841 ggaaacccgg tcactgaggc ccagccgcga caggccggcg gcggccagga tggccgcgtc
      901 cacgcttccc tcgctcactt tgcgcaggcg ggtcgtgaca ttgccccgaa gcggcaggat
      961 ctcaaggtcg ctgcgataag cgcgcagctg cgcggtacgt cgaaggcttg aggtgcccac
     1021 gcgtgcgccg ggaggcagtt gctcgagcgt gaggttgccg gcgcttacga ggacatcgca
     1081 ggggctttca cgctttggaa ccgccccaat gcacagtcct tcgggaagct cggtgggcag
     1141 gactttcatg ctgtggacgg ccaggtcgat gtctccggca aggagggcct cttcgatttc
     1201 cttcgtgaac agccccttgc ctccaatctg cgcgaggggc acatccgtga ttttgtcgcc
     1261 ggatgtccga atgacctgga tatcgacgcg cgtgccaggc tccagagcgc ggagcccgct
     1321 ggcgatgagt tcggactggc acagggccag ttcgctcccc cgggagccga tgacgatgcg
     1381 ttctggcatt ttgtttcctt caacccaaag attcgccgga cgagctggag gacgaaactc
     1441 gggtcctcgg tggcgacttc acgcttaagc tggctcatgg gccgctggag gatggcgttg
     1501 acaatgcgcc gggtcatttg gatcacggct tcgcgctggt cgccggtcag gtcgggatgg
     1561 gcatccagca gcttctggag ttcccgctcg cggatcccgt ggagttcctc cgagatggac
     1621 aggatggccg gttccgcaat gaggctctgc atccactgca cgaattttgt gacgccatcg
     1681 tcgatcatgc gcaggcattg accgatggcc tcatgccgcg ccttcaggtt ttcgtccgcg
     1741 gcctgcttca gggcgtcaac gtcgtagacg tacacgttat cgagctcgtt gaccgccggt
     1801 tcgacgttgc ggggcacggc gatatcgatg acaaacatgg gagcgttggc gcgtttgcgc
     1861 aaggcgcgct ggaaatggcc gggattgagg agcggcgcgg tggctgctgt ggatgtcacg
     1921 acgatatccg cccgggggag ggcctcgtcg agattctcga agggcacggc ttccccgccc
     1981 agttcttcag ccaggacgag ggccttctca tggctccggt tgacgaggag aatgtggccg
     2041 actccgtgcg cgagcaggct tttcagggta ctttcgccca tcttgcccga accgataatc
     2101 atgaccgtct tgtcggccag gtcgataaag atcgacacgg ccagatcgac ggcgatcgac
     2161 gcgacggaaa cctttcccgc tccgattcgc gtctcggtac ggacctgttt cgcgacggaa
     2221 aaggcgcgct ggaacagggc ggacaggacc ttgtcgacgg attcctgttc ctgagcgagt
     2281 gtgaaggctt cgtggacctg tccgaggatc tgggcttcac cgacgacgag gctgtcgagg
     2341 ctcgccgtca cgcgaaagag gtggctgacg gcctcggggc ctttgtgttc gtagagcgac
     2401 ggctcgaatt cccgctcgga gatgttgtgc cagcctgcga tgaaagcgcg gatatcgtcg
     2461 cagagggctg ccgcgtcttt ttcgtggtgg gcataaattt cgacgcggtt gcaggtgctg
     2521 aggatcaccg cggcgccgcc gtgaagccgg ttttggagct cattcagggc cgccgggatg
     2581 gcctctgccg ggaaatgaag ccgttcccgc agagccaacg ggctcgtatg atggctgaga
     2641 ccgaccgcta cgacgcccat gtcgagggct cccagaaata ataatacgac aggttcagca
     2701 cggtgcgcgc caggtagata cccagcatgg ccgaaaagcc gatcaagacg gcaaacgcca
     2761 gtttacggcc gcggagcagg ccgcattggc gcccgtgaaa cacgaaggcg tagaacagaa
     2821 acatcacgac ggactgcagg accttgggag cgaggaacca gtggggcccc agcagcgccc
     2881 ggtccacccg cgcccagatt gcgccggtca gaagcgtggc ggcaaacatc acatacccgt
     2941 ggtatatgag gcgatacagg cttttatcga gccgttcgag cggcggcagg gcttgaaaca
     3001 ggccgacggt acggtgcttt ttgagatggc gcgactggaa gagataggcg acgctggcca
     3061 cgctggcgcc gagaaagaag gcgtaggcta gaaacgccag gccgacgtgc actgcgagaa
     3121 acaggccgcg cagctctttc ggagcatggc cgaaggtcgc gggtgcaagg ctggcgccga
     3181 gaacggccag cacgaaaaga gcgggcgtgt agaatgacgc gaccgcgcgt gtgctgtccc
     3241 gcgcggacac gaacatgacg atggtggcga aaatgacaaa tacgatgagg gcatcggcgg
     3301 cggtggtcat ggggagaatg tgccaggctg ccccgcgcag caggagggcc agcaggaaca
     3361 ggccggcgcc cagtaaggcc agccgccggg tcaggttgag cgcggcctcc cgcttgttgg
     3421 ccaggtacag gactgcagcc gccgtggcgc ccgcgaacgc cagggttccg gcaaacaata
     3481 ccgccagtgt cagggtcgtc atgccgttgt ctccggctgg ttttgtgcga tgacctgctc
     3541 gacccagccg cttcgctggg ccgcatcaag ctggcgaaat cgctgatagc ctgcctcgga
     3601 ggcgagctcg taggcgatcc gcgcgcgaag cgcgggaact gcgacgcggg agcgcgcctg
     3661 cccgcgggcc tggcgcagaa agtccagata ctccccgtag tccgccggga acagcggctc
     3721 caggtcctca cgaaggcgtt ttgcgagggc cggcgcagcg ccgccggtgg atacggcgat
     3781 ctgaaggtcg ccccgccgca gcacggagtt gagatagaac ccgcacactt ccggatcatc
     3841 gactacattg cagagaatac cgcgggcctg gcagatctcc ccgacgctgc ggttggcctc
     3901 ggggtcatcg gtggcggcga tggcgagaaa catgccatcc aggtcgccgg gttcaaactc
     3961 gcgctgcacg agagtggcgc tgagctccgc aaagcccgga gcaaattgcg ggctgaccac
     4021 ggtgacccgt gcgccggctt ctacgagctg gcgcgctttg ggcagggcga tatggccgcc
     4081 gcccgtcaca agacagggcc gttccgcgat cttcaggctg ataagataga gcgacatgcg
     4141 tatgtactga ctgtctgttt attcctgcgc tgcacccggt ttgccggcgg gccggcccgt
     4201 tccggcgccg catcctagaa ggatagcttc cggacggtcc cgctgcaacc gtaaggctgc
     4261 tgcccaccgg gatattccgc agggtgggct ggctgtaact ggtggaggcg ctcgcgtgcg
     4321 agccttcagt cgatgcagcc cggcatttcg ccgtcttgaa ccgccgccgg ctcacccgta
     4381 cactgagcaa cacgatgaaa ccaggcgacg ggcaggatcc agcatggggt ccgccgaaac
     4441 gtaccaggga gacgcacgag caatgtacct ttccatacgc gatgcaatgg tatcaggcga
     4501 cgaattcccg agcccggtcg ccgggctgcg gcatctggct gtcgagaacg ttgaagtacg
     4561 aatgactccg gattatcagg tctgcgctat ggactcgaag gagatgatca cgctggcatc
     4621 cgacgaggac gcgaaggcct acaagaagca tttggacggt ctgggcatcc gggcctgctg
     4681 tttcatgacc gccaaagact tcagcacggg agacaatgag tccaacgcgg cctgggtcgc
     4741 ccgcgccatc gagctggccg acatcatgga aatggcggtg gtccgcatcg acagcgcgat
     4801 gtcgaaagag cgtgagcttg atttcgagaa gcgggtgcag ctgttcgcgg aagggcttgg
     4861 cggcgcactg gaacgcaccg cagggtcgaa agttgccctg ggcatcgaaa accacggttt
     4921 tcagggcaat aatctcgcgt ttcaactcaa tgtctaccaa tcggtgggct cggatcgtct
     4981 gggcgcaacg atggataccg gcaacttcta ctggcgcgga tatcccctct ccgaagtcta
     5041 tgggatcctg cgcattcttg cgccgtatac gaagcacacc catgcgaaaa acatcaacta
     5101 tcccgaagac cagcgcgaaa tcacccggga accgggctgg gaatacgccc ggtattgctg
     5161 cccgctcgac gaaggcgaca tcaatcacgc caaagtcctg cgcatgctgg cggatgccgg
     5221 ctacgacggc gatgtctgca tcgaaaacga gtcggtgggg cgctacaaga cgcccggaga
     5281 acgcatcgag gttcttgagc gcgacgtggc gcattgccgc gccatcatcc aggacctgaa
     5341 gctccggtaa ccgttcggga aatctctgtg ggcgacgaac agccgaagac tgcgaaaccg
     5401 ccgtggcggg gcattgcctg gctcgtttcg ggcctgattc tggcgctggg tgcaagtttt
     5461 ctggcgcagc gcttcgacct tggcaggcgt tttgccctgg atttcaaagg aaagcacgtc
     5521 atcggcattg cggagcggat gggcgacgat tacaacatcc gctacgagca ccccgacggc
     5581 accattttca ggcgtttata caagggcacg ctgagcggat cgccgtcgtc cgatggcaaa
     5641 tttgagatcg ccatcgtttt tgaccccggc gagcccagcc agtttcagcc cgcgggcctg
     5701 tcgtacctgc ccggcgttgc cgcgctgacg ctgttcattc tcggcatggc aagcgtgttc
     5761 aaggggcgca aggtgatgta tgcgcattac ctcgctgcca ccggaaaacc ggaagtcgga
     5821 aaggctggga aacgaggagc agggccgggc agggatcccg cccaggcacg ccgcaaacag
     5881 ctccgcatcg cgctgaccct cgccctcgcg ttcgcgctga tcgtgctcat ctacggctgc
     5941 cctcagttca atatccccgg cttgttcaag aactgagcgc aggtgagtcc ctgcgggatt
     6001 cagggtttcc gggtgatccc catgccgcag agcggcagga gatcggcgta ttcggccgtg
     6061 ccgtagttga acaccgtaaa cccgccggtc ccgagctcac gcgtgatcga gatctgctcg
     6121 atcaacttca cggcatcgag gcgatccggc caggtgcttt gccctatccc cggataacag
     6181 ggcacgttcc ccgtccacgc caactgctga gcggccatgt tacgaaaact gtcgttgtgg
     6241 ggcgtgtagt ccatcggaca cacgaaatcc agatagcctc tatcgcacca cagcttccag
     6301 tcctgcccaa ccttgtcgcg gtcatggggc cagctgcgga aaaccgccgc ggatacttgg
     6361 atgccgggac gggccttccg cgcgccgtcg tgcactccgg ccaccacctt cgtgatgttc
     6421 tgccggcgga attcgagcca cgcgtccagg tcgcgcccgc ctgagccgac atccttcggc
     6481 cagtgggcta cgggccggcc caattgctgc tcaaagcgcg cccggcagcc ggcacagaag
     6541 cagtactcgg aaccggggta gcggatgtaa tcgaaatgga tgccgtcaac atcgtacttg
     6601 cgggcgagct cgaccagcga atcgatctcc agctgctgat tggcgggatg cgacgggcac
     6661 agccattgcg gttgcgccac acccgcgaag ctgacctggg tccggccctc cgatttcatg
     6721 cgatccatga acgatttggg agcgcgtgtg cccatgttcc agcacacctt ccacacgtgg
     6781 cattcgatgc catatttccg gcaggcggcg aggcactgtg cgacctgatc gcctttctgc
     6841 gcaagctcgg ccgccgcggg gagcacatcg ctcggataaa acgccacgcc gccccacacc
     6901 atgttgggca taatggcggt aaagccattg tcggccaggc gacggatcgc ctcgtcccag
     6961 gtcacgccat cgacgccata tgcgctgtgg caccagtagg cccggtgttc accgggaaga
     7021 ggtttctgag cggcgcaata gccttgcagg accgcgcgct ggatctgccc ggctgtctca
     7081 agcgcctcgg ggaaccggcg gctcccgacc agttcggccg cgcgcgcacg gagggattcg
     7141 gcttccgaca ggtagcggtc caccacggga tcctgctgcg agagtgccat gagcccttgc
     7201 cggcacgacg ccagatccgg gtatccgccg atcgcgcccg cgccggcgag tgcgt
//
LOCUS       DAOPBQ010000126         7200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9321_length_7200_cov_3.865080, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000126 DAOPBQ010000000
VERSION     DAOPBQ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7200
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9321_length_7200_cov_3.865080"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PKW60_05655"
     CDS             complement(<1..820)
                     /locus_tag="PKW60_05655"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HPJ98950.1"
                     /translation="MSPEFIDLRSDTVTRPTAGMREAMARAEVGDDVLGEDPSVNALQ
                     LRVADLVGKEAALFVPSGTMANLLAAVSQTRPGDTLVLDESAHPFHFESGGVGMVAGL
                     MTKTLPGTYGVLSPEQVERSLVLQTDHHFSPTTLVSIENTTNRGGGAVYPVETVAAIG
                     DVVHKHGLRLHCDGARIFNACAAIHADVKGYARHCDTLSFCFSKGLGAPVGSILCGDA
                     GAIDRAHRYRKMLGGGMRQAGILATAALYALDHHRDRLSEDHERAKRFRSALDGV"
     gene            complement(949..1323)
                     /locus_tag="PKW60_05660"
     CDS             complement(949..1323)
                     /locus_tag="PKW60_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HPJ98951.1"
                     /translation="MRIRINWPAGALDAILNDSPASHLLAAALPVTSTARTWGHEVYF
                     SVPVEAELDDHPQQIVAPGTVCFWVQGASVAIPFGPTPISVGNECRLVTAVNVIGKID
                     GESTALQSVQDGDSITISALTP"
     assembly_gap    1449..1458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2041..2988)
                     /locus_tag="PKW60_05665"
     CDS             complement(2041..2988)
                     /locus_tag="PKW60_05665"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPJ98952.1"
                     /translation="MRRICFVLFSISSLCAVAHAAPLAAGDSAPPLRIGEWVRPSPVN
                     LFDWTGQNPKPFFLIEFWATWCEPCRAMMPHLAELQNRYAEAGLVIIGVSNEHPSVVH
                     HFAQSRGGDVGYALVTDTAQREVYNAFMRPVGSPGIPHAFLIDRDGSLAWHGVPSAKE
                     IDGRIERLAGQSYTLKSEILAERAERLMARYFAAATRSTNPDETAALGDRIIEWGSAR
                     PDLLREFAWRLLTEPGLESRDVKTALRAATRAHEARPQDFRILETLALAHYAAGDVSQ
                     AATCQAAAVDACPEQTLRLRMKETLDKFRAESSPAATSP"
     gene            3279..6764
                     /locus_tag="PKW60_05670"
     CDS             3279..6764
                     /locus_tag="PKW60_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPJ98953.1"
                     /translation="MRYSNADTILRKESCLIALMACCALGAHAQSLSVVEQDYVGSYV
                     TSGNVWALLELRDASGKPITGLDLSHVTVRESVVDASTGAESTLPQVIDLEAQAATSW
                     KRAGFWEKTIGEVKLDIVFTVDGTSSNRPATAQLRPALHAFIDALLAHHVDFRIALAC
                     FGETPDGLDPSPFFGPMDVPELRKEIDRYPNMGGDWWDPTVAYDSIMNTVWAGFREDA
                     YQVVVAITDIVPQTIYGTFWYANGSTAATLSSMVYFLNSHNIHLFFSHDADTSNDPDY
                     RYYCDPSINPRAGIYESGFEALAQRTDRLGRPLAVDLGWPFDLGNLKAAMRNRLELPS
                     LFLTPCPITESVYLVSWRSNFNWYDNFARMARNPDTLAYRADFTLTLPGTGKTVETFS
                     LYPSRKAVTPSTVYVTDEEGSPQDDSWTDVYAMIGDRTSSYSWQLGPTNGLIALQNTP
                     AGRYRVTVQDWGCFPYHYHSLRGMARMDVDIPPAGAMIDITMTSIDKDSDLARTRGLL
                     QDLADWRSPGDPFQDFVDDANAWLDTLEADGISWWDLIQIKRFEIALSGYAAITEYAH
                     LQTEGAIEDFYQILMDFIAIIQETKDLHDSVDSNWQEASIEILATLVDILFSRGEFTA
                     NRELLEAALDALLEYAEGELITDLKRLVVERIPAGPYKPLIAAMMYTCLDADFENWTP
                     VMEAAQQLALHLAKKEVIGLLAENIVETLFEQIKPANAMEEALEGHVRNLLNGLVSED
                     GFANFGTALNTFAHNAGAHVLQGGINRRVEVVAAIDGLFEKIKAQLPAGMFRDFLIGM
                     TRDLALEAIPTVEKGALKYRIDSSAVVKILVRHALYNVVLKDYYAEEIKAGLYETLYR
                     AQAYVPVGTGRYQYQSSMFQDFVAFRERFSWYDSIHDLAWNALRVQKDIKGWGNALTA
                     LNSCLDAVSVPLEFFAGWYPPLQDTADAVSDFVDVLDGAQIVARSIEFGLRLDCLHTL
                     AGFSEPFYETIFPTPATDVYLMGVSPVEHDYGKTKVTRSAMICPVTIWNAGNKALKVS
                     TMVLSDTKNFSFAGPASLSPVIAPGHSHTVHVKFDPSSTGEFTTALTVYCGMTTPPQK
                     TITLRGVGTYLDRGVPGDTNSDTLLNAIDVQLVINAALGLFNYAHTDLNSDGKVNAVD
                     VQLIINAVLGLPVPF"
     gene            7048..>7200
                     /locus_tag="PKW60_05675"
     CDS             7048..>7200
                     /locus_tag="PKW60_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPJ98954.1"
                     /translation="MAEGILQGKTGVILGVANERSIAWACAQACSAAGARLIFNYLGE
                     PLEKRVK"
BASE COUNT         1537 a         2024 c         2126 g         1503 t
ORIGIN      
        1 ggacgccgtc cagtgccgag cgaaaacgtt tcgcgcgctc atggtcttcg gatagccgat
       61 cgcggtgatg atccagcgca tagagcgccg ccgtggccag gatgcccgcc tgccgcatgc
      121 cgccaccgag catcttgcgg tagcggtggg ctcgatcgat ggcgccggca tcaccgcaca
      181 ggatggaccc cacgggcgcg ccgaggcctt ttgagaagca gaaggacagg gtatcgcaat
      241 ggcgtgcata gcctttaacg tccgcgtgga tggctgcgca ggcgttgaag atgcgcgcac
      301 catcgcaatg caggcggagc ccgtgcttat gcaccacgtc tccgatggcg gcgacggtct
      361 caaccggata cacggcgcct cccccccggt tcgtcgtgtt ctcgatcgac accagcgtgg
      421 taggcgaaaa gtggtggtcc gtctgcagca cgaggctgcg ctcgacctgt tccggggaca
      481 ggacgccgta cgtgccgggg agggttttcg tcatcaatcc agcgaccatg ccgacaccac
      541 cggattcgaa atggaacgga tgcgcggatt catcgagcac cagggtatca ccggggcgcg
      601 tctgagaaac ggccgcgagc agattggcca tagtacccga aggaacaaac aaggcagctt
      661 ccttgcccac caggtccgct actcgcagct gaagggcatt gacgctcgga tcttcgccga
      721 gcacgtcgtc gccgacttcg gcccgcgcca tggcctcgcg catgcccgct gtgggccgtg
      781 taaccgtgtc gcttcgtaaa tcaatgaatt cgggtgacat gtccccacct cagcaccatt
      841 catgcgcatg ggcttgcact ggaacgcttt catccaccgg gacagcatac ggcctttcgc
      901 tgcatggatg catatttttt ggatgggaac gggaggaatc gcgtgccgtc agggcgtgag
      961 agcactgatc gtgatggaat cgccgtcctg aacggattgc agcgcggtgg attcgccgtc
     1021 gatttttcca atcacattga cggccgtcac caggcggcac tcattgccca ctgagatggg
     1081 agttggaccg aaaggaatcg cgaccgaagc gccttgcacc cagaaacaga cagtacccgg
     1141 agcgacaatc tgctggggat gatcgtccaa ttcggcttcg accggaacgg aaaaatacac
     1201 ttcatgaccc caggtgcggg cggtagaggt caccggcaat gcagcagcca gcaggtggct
     1261 ggcaggcgag tcattcaaaa tggcgtccag cgcgccggcg ggccagttaa tccgtatacg
     1321 catagtccat ggacatgccc ctcagccagt cactgggcga agagccgacg ttgcggatgg
     1381 tatggaccac atgggcggga accaggatct cttctccggg cttgagctcg aagatctctc
     1441 ctttcattnn nnnnnnnncg attgccataa tgagcttgtc ggcgtcgtgc gcgtaatccg
     1501 tccaggcctg ccccggtgga tcgcttcggc gttcacacgt gaaccctcgc gcatgccagt
     1561 cggcgtcgac ttgagcctca ttgacgagcg atttgttcag gtcttcgatt acttcgccca
     1621 taagaaaacc tttcccaagt ctcagtttcg tgcttttcga gatctcaatt gccaagttta
     1681 cgggattttt aggttcaatc cagcttcatt gtacgacata agacgagatt tagcaagaga
     1741 tattcatgtt ttgtacattt tggcatcgct tttgtccgca agattcgaaa agttggaaga
     1801 aatgcccggt tttggcatat aaatccctgc aattcagcaa gttgataccg caaacagcag
     1861 agcttccagc acatgcaatg gatacatttt tcattaaaac atggattttt gtcaacaaac
     1921 taccttatac aaatatcgcg aatatcaatc aaatccggca ttcagggaaa acgccatgga
     1981 tccgaagtca cagttcatgt gcaagtggcg aattttcaaa accttacttg gactgtgcct
     2041 tcatggggac gttgcagctg gggaggattc cgcacgaaac ttgtcaagtg tttctttcat
     2101 gcgcaggcga agagtctgct cggggcaggc gtcgacagcg gccgcctggc aggtggcggc
     2161 ctggctcacg tcgcccgcag cgtagtgggc gagggcaagc gtttcgagga tgcggaagtc
     2221 ctgtgggcgg gcttcgtgcg cgcgggttgc ggcgcgcagc gcggtcttca cgtcgcggga
     2281 ttcgaggcct ggttcggtaa gaaggcgcca cgcaaattcg cgtagcaggt cgggacgtgc
     2341 gctcccccac tcgataatcc gatcgcccag ggctgccgtt tcgtcaggat tggtgctacg
     2401 ggtcgcggca gcaaagtaac gcgccatgag ccgctcggcg cgttcggcga ggatttcgga
     2461 tttcagggta tagctttggc cggccaggcg ctcgatgcgg ccgtcaatct ccttagccga
     2521 ggggacgccg tgccaggcga gactgccatc ccgatcaatc aaaaaggcgt gcgggatgcc
     2581 cggcgagcca acaggcctca tgaacgcatt gtatacttcg cgctgagcgg tgtcggtaac
     2641 gagcgcatac cccacatcgc ccccccggga ctgggcgaaa tgatggacaa cggacggatg
     2701 ctcattactc acgccgatga tcaccagccc tgcttcggcg tatcgattct ggagctcggc
     2761 gagatgcggc atcatcgccc ggcagggctc gcaccacgtc gcccaaaact cgatcagaaa
     2821 aaatggtttc ggattttgcc ccgtccagtc gaagagattc accgggctcg gacggaccca
     2881 ttcgccgatc cgcaacggcg gagcggaatc gcccgccgcc agcggtgcgg catgtgccac
     2941 cgcgcacagg ctggaaattg aaaacagcac aaaacatatc ctgcgcatga cagtctccct
     3001 gcttgccggc ctactctatc cccgcgaagc gccccagtgc aaggccatca ggaagtcctg
     3061 cactagcaat atggccatct atacggcgtc agggcgggtt cccaggagcg tttcggccca
     3121 gaactcaagc ctcgatttta ttgtttttag ggtattgccc gcttttagcc ggcgtactat
     3181 actgactctg ataaatcgcg ccatgagttg ggcgactcat gatgacatgg ccttgaagcg
     3241 cgacaggagc tgcatcggcg ttgagggagg ggtctgcgat gcggtattca aacgctgaca
     3301 cgatcttgag aaaagagagc tgtttgattg ccttgatggc atgctgcgcg ctcggggcgc
     3361 atgcacaaag cttgtccgtg gttgagcaag actatgtggg ttcgtatgtc acgtctggaa
     3421 atgtgtgggc cctgcttgaa cttcgggatg cctcgggaaa acccatcacg ggactggatc
     3481 tatcccatgt gactgtgcgg gaatccgtcg tggacgcatc cacgggcgcg gagagcacgc
     3541 tgccgcaagt gatcgatttg gaggcccagg ccgcaacgtc gtggaagcgc gcgggattct
     3601 gggaaaagac gattggggag gtgaagctcg acattgtatt cacagtcgat ggcacctcaa
     3661 gcaatcgccc ggcgaccgcg cagcttcggc cggccttgca tgcatttatc gacgcgctgc
     3721 tcgctcatca tgtggatttc cgcatcgctc tcgcctgctt tggcgaaact cccgacggtc
     3781 tcgatccttc cccattcttc ggcccaatgg atgttccaga gttgagaaag gagatcgacc
     3841 gatacccaaa catgggcggc gactggtggg atcccacagt ggcctatgat tcaatcatga
     3901 acacggtatg ggcaggtttc cgtgaagatg cctatcaggt ggttgtggcg atcacggaca
     3961 tcgtgccgca gacgatttac gggacgttct ggtatgccaa cggttccacg gcggcgacgc
     4021 tttcgagcat ggtctatttt ctcaactcgc acaatataca cctgtttttc agccacgatg
     4081 ccgacaccag caacgacccc gactaccgtt attactgcga cccgagcatc aatccgcggg
     4141 caggcattta tgagagcgga ttcgaagccc tggcacagcg aacggacagg ctgggcaggc
     4201 ctctggccgt ggatctcggc tggcccttcg acttaggcaa cctcaaagcg gcgatgcgaa
     4261 accgccttga actcccctcg ctctttttga ctccctgtcc catcaccgaa tcggtgtacc
     4321 tggtttcatg gagaagcaat ttcaattggt atgacaactt cgcgcgcatg gcacgcaatc
     4381 cggacacgct cgcataccgc gccgacttca cgctcacact gcccgggaca ggaaagacgg
     4441 tcgagacgtt cagtctctat ccttcaagaa aagcggtgac tccatccacc gtctacgtga
     4501 cggatgaaga gggcagcccg caagatgatt cctggaccga cgtctatgcc atgataggtg
     4561 accgaacgtc ctcgtattcg tggcagctgg gcccaacgaa cggtttgatc gctctccaga
     4621 acacacccgc aggccgctat cgggtgaccg ttcaggactg gggctgcttc ccgtaccact
     4681 atcattccct tcgcggaatg gcgcgaatgg atgtcgatat tccgcccgca ggcgccatga
     4741 tcgacatcac aatgaccagt atcgacaaag actccgattt ggcgcggacc cgcggattgc
     4801 ttcaggatct cgccgattgg cgctcgccgg gagacccttt ccaggatttc gtcgatgacg
     4861 ccaatgcctg gctggatacg ctggaagccg acggtatttc ctggtgggat ctgattcaga
     4921 tcaagcgatt tgagatagcg ctgtcgggtt acgcggcgat taccgaatac gcccatctgc
     4981 aaacagaggg cgccattgaa gacttctatc agatcctgat ggatttcatt gcgattattc
     5041 aggagacgaa ggatcttcat gattcggttg acagcaactg gcaggaggct tcaatcgaga
     5101 ttctggccac gcttgtggac attctgtttt ctcgcgggga gtttacggcc aaccgtgaat
     5161 tactggaggc ggcattagac gcattgcttg agtacgcgga gggcgaactc atcacggacc
     5221 tgaagaggct ggtggtcgaa cggattccgg ctggaccgta taagccgctg atcgccgcca
     5281 tgatgtacac ctgcctggac gccgattttg aaaactggac tccggtaatg gaggcagcgc
     5341 aacagctggc gcttcatctg gccaaaaagg aagtcatcgg cctgctcgcc gagaacattg
     5401 tggaaacact gtttgagcag attaagccgg cgaacgccat ggaggaagcg ctcgaaggac
     5461 acgtgcgcaa tctgctgaac gggctggtaa gcgaggatgg attcgcgaat ttcggcactg
     5521 cgctgaacac gtttgcccat aatgcagggg cccatgtgct tcagggaggc atcaaccgcc
     5581 gagtggaagt ggtcgcagca atcgacggtc tgtttgaaaa aatcaaggcg caattgcccg
     5641 cgggcatgtt tcgggatttc ctgatcggca tgaccagaga tttggcgctg gaagccatcc
     5701 ccaccgtaga aaaaggcgcg ctcaagtacc gcattgacag cagcgcggtt gtcaaaatac
     5761 tggtccggca cgcgctctac aacgtggtgt tgaaagacta ttacgcggaa gagataaaag
     5821 cagggctgta tgagacgctg taccgcgcgc aggcctatgt gccggtcggg acgggacgtt
     5881 accaatacca gtcctccatg ttccaagatt tcgttgcgtt ccgcgagcgg ttttcctggt
     5941 acgactcgat ccacgatctg gcctggaacg ccttgagagt ccaaaaggac atcaagggct
     6001 ggggaaatgc gctgacggca ttgaactcct gtctggacgc cgtaagcgtt ccgctcgagt
     6061 ttttcgcagg gtggtatccc ccgctgcagg acacggcaga tgctgtttcg gattttgtag
     6121 atgtgctgga cggggcgcag attgtcgccc gctcgatcga gttcgggtta cggctcgact
     6181 gcctccatac gcttgcgggc ttctctgagc cgttttatga gacgatcttt cctacgccgg
     6241 cgacagatgt ctatctgatg ggtgtgtcgc cggtggagca cgattacggg aagaccaagg
     6301 tcacgcgcag cgcaatgatt tgcccggtga ccatctggaa tgcgggcaac aaggcgctca
     6361 aggtcagcac tatggtgctg tcggatacga agaatttttc gttcgccggg cccgcctcgc
     6421 tttccccggt gattgcgcca gggcacagcc ataccgtaca cgtaaaattt gacccgtcca
     6481 gcacgggcga gtttacgaca gcgctcaccg tctattgcgg catgacaact ccccctcaga
     6541 aaaccatcac cctcagggga gtgggcacgt acctggaccg tggagtgccg ggagacacca
     6601 acagcgacac gctgctgaat gccattgacg tgcaactggt catcaacgcg gccctgggtc
     6661 tcttcaatta tgcccacacc gacctcaaca gcgatgggaa ggtcaatgcc gtagatgtac
     6721 agctgatcat caatgcagtg ctcggcttgc ctgtaccctt ctaaaacgtg ggcaggccgc
     6781 acacagcggc tccagcgcca gtcatgcgaa cagagtcgcg gagccaggcc gggcaggtgc
     6841 cctgggcgtg ccccctgttc ttcctccggc ttcgcaggca ccgtaacacg aacagggcgg
     6901 gcgccatccg tgccccgcgc gctaaaaaaa ccggagtatg tgaattttcc atcacgaagc
     6961 cagatttgat aggctttctc agggatccgc aaaactgttc tgttcgccag gaattctggg
     7021 gggcttgatt atcaaggagg atggcatatg gcggaaggaa tattgcaggg caagaccggg
     7081 gtgatcctgg gcgtcgcgaa cgagcgttcg atcgcctggg cctgcgcgca ggcctgctcg
     7141 gccgctggcg cgcggctgat cttcaattat ctcggggagc ctctggaaaa gcgagtcaaa
//
LOCUS       DAOPBQ010000127         7197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9325_length_7197_cov_4.120835, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000127 DAOPBQ010000000
VERSION     DAOPBQ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9325_length_7197_cov_4.120835"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1485
                     /locus_tag="PKW60_05680"
     CDS             208..1485
                     /locus_tag="PKW60_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPJ98955.1"
                     /translation="MENPGIEQEAPPKTPQQALSLSEIQPRLKELLETGDTRATLAVD
                     LALTQAVHDGASDVHFEPWTDSLAVRFRIDGILHDIAHIPDSHKARITARIKVMAQMV
                     VYQKDQPQDGRIPPNQTSSGYSMRVATFPTINGEKIVVRILGTSKQMLEVDDLGFEAS
                     VAETLRSLVRRPQGVILLTGPSSSGKTTTIFALLRELLRTQIATTHIVTIEDPVEYRL
                     DRVTQIQVNPRTGVTFQNALRSILRQDPEVIMVGEIRDAETAKMAVQAGLTGHLVIST
                     IHSGTASGVFARLLDMGIEPYLLASSVTGVLAQRLARVNCPNCLETYTPTPDLLTRFG
                     KPPKNQPYKRGKGCAQCQHIGYRGRTALGELLLVNQDIANLILQRPTTNAMQEAAAAH
                     HMRSLFRVGLEAAYKGITTPEELSRVLPPPEVV"
     gene            complement(1615..1875)
                     /locus_tag="PKW60_05685"
     CDS             complement(1615..1875)
                     /locus_tag="PKW60_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98956.1"
                     /translation="MDKHIIYGVHVTNRMRKATGIQQLFSEYGCNIKTRIGLHDVGPD
                     YCSPSGVILLEMFGDEAACDEFRKKLSTIDGIEVQQMIFDHD"
     gene            complement(1954..3354)
                     /gene="hydG"
                     /locus_tag="PKW60_05690"
     CDS             complement(1954..3354)
                     /gene="hydG"
                     /locus_tag="PKW60_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPJ98957.1"
                     /translation="MLKHTDPVMFIDEEHIEATLTRESRSDAARIRDILARARELGGL
                     ELDEIAALTGVSDPELLGEMFEAARYVKEEIYGRRLVLFAPLYISNLCANECLYCAFR
                     KTNKEVKRRALTQEEIAHEVRILIDQGHKRILLVAGESYPVEGFDYVLDSIATIYNTR
                     HGNGEIRRVNVNVAPLTVDEFRRLKDANIGTYQLFQETYHRETYARMHVDGLKRDYNW
                     RITAMDRAMEAGIDDVGIGILFGLFDYRFEVLALMQHIRHLEAQFGVGPHTISVPRLE
                     PALNAEIASHPPHPVSDIDFRKIVAILRLAVPYTGIIMSTRETPNIRRETFALGVSQI
                     SAGSRTNPGGYAEDEEYDCSQFSLGDHRQLDEVIRDVASLGYVPSFCTGCYRLGRTGA
                     DFMDLAKPGKIKDHCDVNAVSTFMEYLCDYATDETFAIGEQCIATALSNMTPERRATA
                     QVLVDRVKAGKRDCYV"
     gene            complement(3426..4730)
                     /locus_tag="PKW60_05695"
     CDS             complement(3426..4730)
                     /locus_tag="PKW60_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HPJ98958.1"
                     /translation="MSSIDEIVRFIDLARVTVPLHEFTAQERAALDNVNHRVASGESL
                     ADVMDFVFESTRDLFPCDRLSISFVQEDDRVASHWTRALYAPLLLQPGYSEDLRQSAL
                     ARVRDTGCVRIIDDLNAYLEVKPRSASTQLLVREGVRSSMTCPLSVNGRNAGFLFRSS
                     RNPGAYAMHEVLLHSALAERLSQAVEKAYRIEQLTAANQAYAEMLGFVSHELKSPLGA
                     IVMDASVLEQGFLGRLEPPQIERLQKITGKARHLLTLIQDYLDLARVEGGGLSYRPRP
                     GVDFLGDVVQPVLDGLQTQLEVQRTRFAQDFPEAPSPVECDPGLLNIVMANLLGNAVK
                     YGNEEGLIRLRVTQPEGQLAVSVWNAGPGFPKSERSKLFKKFSRLQSRELMARKGSGV
                     GLYTCWRIIQAHGGRIRANSEPGAWAEFSFEIPQPLPSNVRG"
     gene            5579..7021
                     /locus_tag="PKW60_05700"
     CDS             5579..7021
                     /locus_tag="PKW60_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HPJ98959.1"
                     /translation="MKRLILMVMLSLFPRLLAAQELDISSLVGQTWYGVYMSGQKSGY
                     AVNNVAKEEDGSVTVSEDAQFRITMEGVKQDMRIFSERTYDASGALSAVKTSVETLGA
                     ESQFDAVVRGDALFMRSVVGGEALEVELPRPKESLRDAFKQVELIQNGGAVGNELHYT
                     IFEPMYRQEIAGVSKIIAIEERVFDGVPTKVYKIKNSLVDMGIDSISYVTQDGTTLED
                     EVSRLIVTRLEPEEIAKDVNYSNDVIVSNAALIDTAIEKPRDRQFLKLRITGPLMEAH
                     LFNDERQYLAPKDGAFEFSAHLISLDDFQPATLPITDEAVQPWLKPSLFVQSEDPRLI
                     EKAREIVGDETDAFAVSKRLCNWVYNNVRTTFSAQLSNALEVLDHMEGDCTEHSVLFI
                     GLARAAGIPAREVAGLIYVESARPGFYFHQWATVWVGKWIDVDPTFNQPLADVTHIKL
                     AEGDLFHQAKLIPIIGQIQVQVVEGAPSSE"
BASE COUNT         1395 a         2172 c         2233 g         1397 t
ORIGIN      
        1 cgggccagcg agtctccccc cgtgcttcgc ggcagtgact tcctctgcgg aagtgccttt
       61 tgagtcgaat ctgtgcgata atcagaccaa tcaacccggg aatcccgggg tcacaagcat
      121 aataacctga ataacgggct gcatttccgt ctggggaatg gcgacgtttc acggcatctc
      181 ctgtgcggca ggcccgtaag gagtggtatg gaaaacccgg gcatcgaaca agaagccccg
      241 ccgaagacgc ctcaacaagc cttatccctc agcgagattc agccgcgcct caaagaattg
      301 ctggaaacag gcgatacccg ggccacgctg gcggtcgatt tggccctgac gcaggcggtc
      361 cacgacggcg caagcgacgt ccacttcgaa ccgtggaccg acagtctggc cgtacggttc
      421 cggatcgacg gtattctcca cgatatcgcc catatccccg attcccataa ggctcgcatc
      481 accgcccgaa tcaaagtgat ggcccagatg gtcgtctatc agaaagacca gccccaggac
      541 gggcgcatcc cgcccaacca gacatcgagt ggttattcca tgcgcgtggc cacgtttcct
      601 accatcaatg gggaaaagat cgtggtgcgc atcctcggga cctcgaagca aatgctggag
      661 gtcgatgatc tcgggtttga agcttcggta gcggagaccc tgcgcagcct ggtgcggcgt
      721 ccccagggcg taattctgct cacggggccc agttccagcg ggaaaaccac gaccattttc
      781 gcgctgctgc gcgaattgct gcgcacgcag atcgcaacga cccacatcgt caccattgaa
      841 gatcccgtcg aataccgcct cgaccgggta acgcagatcc aggtgaatcc acgaacgggg
      901 gtgacgttcc agaacgcgct gcgctccatt ctgcggcagg accccgaggt gatcatggtt
      961 ggtgaaatcc gcgatgccga aacggccaag atggcggttc aggccgggct gacggggcat
     1021 ctggtgatca gcaccatcca cagcggcacg gccagcggcg tcttcgcgcg cctgctcgac
     1081 atgggcatcg aaccctacct gctggcctcg agcgtcacgg gcgtactggc ccagcggctg
     1141 gcgcgggtca actgccccaa ttgcctggaa acctacacgc cgacgcccga cctgctgacc
     1201 cgttttggca aacccccgaa aaatcagccc tataagcgcg ggaaaggctg cgcacaatgc
     1261 cagcacatcg gttatcgcgg ccggaccgcc ctgggcgagc tgctgctcgt caaccaggac
     1321 atcgccaacc tgattctcca gcgtccgacg accaatgcga tgcaggaggc cgccgcggcc
     1381 catcatatgc gctcactgtt tcgcgtcggc cttgaagccg cctacaaagg catcaccacg
     1441 ccggaggagc tgtcgcgggt gctgccgccg cccgaggtcg tgtgaccgag gcgctcgaag
     1501 ccggaaccgg ccccgggaaa acaggtgtgc cgccggctcc actgccgagg gcgttttgaa
     1561 ctccccaaat cctcaggaac gccgggattg gagcgtgccg gtgcggcttg cccgtcagtc
     1621 gtggtcgaag atcatctgct ggacttcgat gccgtcgatc gtggagagtt ttttgcggaa
     1681 ctcgtcacag gccgcttcat cgccgaacat ctcgaggaga atcacgccac tgggcgagca
     1741 gtaatccggg ccgacgtcat gcagcccgat gcgggtcttg atgttgcacc cgtactccga
     1801 aaagagctgc tggatgccgg tagctttgcg catgcggttg gtcacatgca cgccgtaaat
     1861 gatgtgttta tccatggcgg ttttctccct ccgtttcccc gaatgcccac gcccccagtg
     1921 tgcacgggag cgcgtcgtga atcaagcagc gcgctagacg tagcagtcgc gttttccggc
     1981 cttgacccgg tcgaccagga cctgtgcggt ggcgcggcgc tccggggtca tgttcgagag
     2041 cgcggtcgcg atgcactgtt cgccgatggc gaaggtttcg tccgtggcgt aatcgcagag
     2101 atattccatg aaggtgctca cggcattgac gtcgcaatgg tctttgatct tgccgggttt
     2161 ggcgagatcc atgaaatctg cgcccgtgcg cccgaggcgg taacagccgg tgcagaacga
     2221 ggggacatag ccgagcgagg cgacgtcgcg gatgacttca tcaagctgac ggtggtcgcc
     2281 gagagaaaac tggctgcagt cgtattcttc gtcctcggcg taaccgccgg gattggtgcg
     2341 gctgccggca gaaatctgag aaacaccgag ggcgaaggtt tcgcggcgga tgttcggcgt
     2401 ttcgcgggtc gacatgatga tgccggtgta gggaaccgcg aggcgcagga tcgccacgat
     2461 cttgcgaaaa tcgatgtccg agacagggtg cggcggatgg gacgcgatct ccgcgttcag
     2521 ggccggttcg agccggggca cgctgatggt gtgcgggccg acgccgaatt gcgcttcgag
     2581 atggcggatg tgctgcatga gcgccagcac ctcgaaacgg taatcgaaca atccgaagag
     2641 gatgccgatg ccgacatcat cgatcccggc ttccatggcg cggtccatgg cggtgatccg
     2701 ccagttgtag tcgcgtttga ggccgtcgac gtgcatccgg gcgtaggtct cccggtggta
     2761 ggtctcctgg aagagctggt aggtgccgat gttggcgtcc ttgagccggc ggaattcgtc
     2821 gaccgtaagc ggggcgacgt tgacattcac ccggcggatt tcgccgttcc cgtgccgggt
     2881 gttgtagatg gtggcaatgg aatccagcac gtagtcgaag ccttcgacag gataggactc
     2941 gccggcgaca agcaggatgc gtttgtggcc ctggtcgatg aggatccgga cttcgtgggc
     3001 gatttcctcc tgcgtcaggg cgcggcgctt gacctccttg ttggttttgc ggaaggcgca
     3061 atacaggcat tcgttggcgc agaggttgga gatgtaaagc ggcgcaaaca ggaccagccg
     3121 ccgcccgtag atctcttcct tgacgtaccg ggcagcctca aacatctcgc cgagcagctc
     3181 gggatcgctc acgccggtca gggcggcgat ttcgtccaac tccagaccgc cgagctcgcg
     3241 ggcgcgggcg aggatgtcgc ggatgcgcgc ggcgtcactg cgggattcgc gggtgagggt
     3301 ggcttcaatg tgttcttcgt caatgaacat aacgggatcg gtatgcttca gcatggtcca
     3361 gactctctct tgtgtttatg gcgcgcttgg cggccagccg gtacgggcgc caggtggcat
     3421 cgggtttatc cgcgaacgtt cgacgggagc ggctgcggaa tctcaaagct gaattcagcc
     3481 caggcgccgg gttccgagtt cgcccggata cggccgccat gcgcctggat gatccgccag
     3541 catgtgtaca accctacgcc ggagcctttc cgggccatca actcccggga ctgcaatcgc
     3601 gagaacttct tgaagagctt cgagcgttcg cttttgggga agccagggcc ggcattccag
     3661 accgagactg ccagctggcc ttctggctgc gtcacgcgca agcggatcag accttcttcg
     3721 ttgccgtatt ttaccgcatt tcccaacaga tttgccatca caatgttcaa cagccccgga
     3781 tcacactcca ccggcgaagg agcttcgggg aaatcctgtg cgaaacgcgt gcgttgcacc
     3841 tcgagctgcg tttgaaggcc gtccagcacg ggctgaacga catcgcccag gaaatcgacg
     3901 cccgggcggg gccggtaact caacccgccg ccttcgacgc gggccagatc caggtagtcc
     3961 tgtataagcg tcaggaggtg cctcgcttta ccggtgatct tctgaaggcg ctcgatctgc
     4021 ggaggctcga ggcgccccag gaagccctgt tccagcacgc tggcgtccat gacaatggcg
     4081 ccgagcgggc ttttcagctc atggcttaca aaccccagca tttcggcata cgcctggttg
     4141 gccgcggtga gctgttcgat gcgataggct ttctcaacgg cctgactgag ccgctcggcc
     4201 agcgcgctgt gcaggaggac ttcgtgcatg gcataggcgc cggggttgcg cgagctccgg
     4261 aagagaaagc ccgcgttgcg cccgttgacc gagagcgggc aggtcatgct ggaacgtacg
     4321 ccttcgcgca ccaggagctg cgtcgaggcg ctgcgcggct taacctcgag gtaggcgttc
     4381 agatcgtcga taatgcggac gcaacccgta tcgcgcacgc gggcgagggc gctctgccgc
     4441 aggtcttccg agtatccggg ctgaaggagc agcggagcat acagggcgcg ggtccagtgc
     4501 gacgccacgc ggtcatcttc ctgaacgaat gagatgctga gccggtcgca gggaaacagg
     4561 tcgcgagtcg actcaaacac aaaatccatg acgtcggcaa gtgattcgcc cgacgcaacg
     4621 cggtggttga cgttgtcgag agcagcccgt tcctgggcgg taaattcatg caggggcacg
     4681 gtgacccggg ccaggtcaat aaagcggacg atttcgtcga tactgctcat gtttcagccg
     4741 tatttccttg tttcctgtgc ggcgggcctg ctgcaggaca gcgctctctg tgcctgtgca
     4801 tgcctctcgg agccgtctgc cccgtgccgt cgccctgccc cgccgggttg ccatctatag
     4861 tacggccttc gatgacgcac attccatgcc gcgcattcga atgccttgaa accaccgaca
     4921 gcctgcgaca gtccaccccc ggccacgcgg gacgcatcgt catgccctaa accatccctg
     4981 ttttcgcagc gtgcatgagc aggcccctgt gcctgccgtg gcttcgggtc aacgcgtgcc
     5041 gatgggatgg gcggccacag gaggccgccc ctgcaatccg gatcgcgaac gcctggggct
     5101 gccgtgtcaa cctcagtttt cggagcgcgt aggggcaggc atcttgcctg ggttcttctc
     5161 ttgcgtagca cggaccaaga actttatgta cggtgcagct gccactcgcc cgcgggagac
     5221 acaagactcc ggatcctgag agaatgagat gcggcgcgcc tgtccccctg ctggcgaagg
     5281 taagtctgca ggcccgagcg ttcaataaaa tgcggctcac tgtccattgc tggcggaggt
     5341 gaatcggcgg cagccggttt gggggtggag ttttggagat ggattggggc ccccggagcc
     5401 cacttgcagc gctgttgtac cgagccattt caagactgga taagccgccc attcccgcga
     5461 gatttcataa tatatggaaa ttcggtacaa tgtccggtgg tctgtgcctg ggtgctgtgg
     5521 cgcggccacc tgtcctggtg gcagcaacga agaacgcgct gttccggaga acatgcgaat
     5581 gaaacggttg attctcatgg taatgttaag cctctttccg cgcttgctcg cggcgcagga
     5641 actcgatatt tcctcgctcg tgggtcaaac ctggtacggg gtatacatgt ccggccagaa
     5701 gtccggttat gccgtcaaca atgtcgcgaa agaagaagat ggcagcgtta cggtcagtga
     5761 agacgcccag tttcgcatta ctatggaagg cgtcaagcag gacatgcgca tcttcagtga
     5821 gcgtacctac gatgcgagcg gcgcgctgag cgcagtcaag accagcgtcg aaaccctggg
     5881 cgccgaatcg caatttgacg ccgttgtccg aggagatgcg ctgttcatgc gcagtgtggt
     5941 cggcggcgaa gctctggaag tggaattgcc ccgccccaag gaatccctgc gcgacgcttt
     6001 caagcaagtt gagctgatcc agaatggcgg agcggtgggc aatgaactgc attacaccat
     6061 cttcgagccc atgtaccggc aggagatcgc cggcgtcagc aagatcatcg ccatcgagga
     6121 gcgcgtcttt gatggcgtgc ccaccaaggt ctacaagatc aagaattctc tcgtcgatat
     6181 gggcattgac tccatttcgt acgtcaccca ggacggcacg accctcgaag acgaggtgtc
     6241 gaggctcatc gtcacgcgcc tcgaacccga ggagatcgcc aaagacgtca attacagcaa
     6301 cgacgtcatt gtgtccaatg cggccctgat cgatacggct atcgaaaagc cccgcgatcg
     6361 ccaattcctg aagctgcgca tcaccggccc tctcatggaa gcccatttgt tcaatgacga
     6421 gcgccagtat cttgcgccta aggacggagc ctttgagttt tccgcccacc tcatatccct
     6481 ggacgatttt cagcccgcga ccctgccgat caccgacgag gcggttcagc cctggctcaa
     6541 accatctctc tttgttcaga gtgaagaccc caggctcatc gaaaaagccc gggaaatcgt
     6601 gggcgatgaa accgacgcct tcgccgtctc caaaagactc tgcaactggg tgtacaacaa
     6661 cgttcgcacc acgttctccg cccaattgag caacgcgctt gaggtattgg accacatgga
     6721 gggtgattgc acggagcaca gcgtgttgtt catcggcctg gcccgggcgg cgggcattcc
     6781 ggcgcgcgaa gtggccgggc tgatttatgt cgaaagtgcg cgccccggtt tctacttcca
     6841 ccagtgggcc acggtctggg tcggcaagtg gatcgatgtt gatcccacgt tcaatcaacc
     6901 cctcgctgac gtcacccaca tcaagctggc cgagggcgac ctgtttcatc aggccaagct
     6961 catccccatc atcggccaga tccaggtgca ggttgtcgag ggtgcgcctt caagcgaata
     7021 actgcgccta tggaaggcac ggcccccaaa tccggaacca ggccagccgc cgcccgtaaa
     7081 cggcgatggc cgagccgggt cctgagcgtg atcctgctcg gtctggggct tgtgctgctg
     7141 gcgctcgcca ccgggtaccc gcaactccac ctgctgcaat acgcccttcg ccgtacg
//
LOCUS       DAOPBQ010000128         7185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9344_length_7185_cov_4.667321, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000128 DAOPBQ010000000
VERSION     DAOPBQ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9344_length_7185_cov_4.667321"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PKW60_05705"
     CDS             complement(<1..770)
                     /locus_tag="PKW60_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPJ98960.1"
                     /translation="MPESVTGPSSNPFDITRDVTAIILGGGRGSRLRPLTSERAKPAV
                     PLCGRYRLVDIPISNCIHSKINHVFLLTQFNSASLHRHISHTYKFDAFSQGFVEILAA
                     EQTYQSGDWYQGTADAVRKQLRHFCNLNAKYFLILSGDQLYSMDYGAILRTHIDKGAE
                     ITVAALPVARDAARGFGVMQVDPNGRLVNFVEKPSDEAQCQELVTPKAVFDDFGLDSE
                     GRNYLGSMGIYVFNADLLPGLLLEHVDWNDFGHHVLPRS"
     gene            1048..2049
                     /locus_tag="PKW60_05710"
     CDS             1048..2049
                     /locus_tag="PKW60_05710"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPJ98961.1"
                     /translation="MADFLLRMLDRLEALGIRTQKLRWKLYQWEKKREAARERGTALP
                     TRLQWMTYPHKFCFNCNALNDRDARRCDKCGARLPSFLGYKLFRLFGIITPQGGAPTV
                     MIFMAVMIGLFGLSLLLGGLQTLVGPDFKTLLRFGGWMPLMMRDPLQYWRALAFGLYH
                     AGLIHIVFNLFALSQIGPIMENQVGRSRMLVVITVCQLTAAAGSFIWYGLAQGMPLVP
                     TLGASGWIFGLIGFGIAYFHTGGPGLRIYRDALVRWVVYMLIFGFIVPRVNNAAHLGG
                     LAGGLLLGWFPEAHPRRSALVRHFWSAAFWISLFLWLVTAVFLAYSIAANPFKELFQ"
     gene            complement(2172..5369)
                     /locus_tag="PKW60_05715"
     CDS             complement(2172..5369)
                     /locus_tag="PKW60_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPJ98962.1"
                     /translation="MKRGIHMAVALGVTALMAEASAQHLLVRTTARTASRQETRQTLH
                     VVDMAQGDIFPWRATLPGMECASRLTICRDNVCAFVATRKLWPHPEGWPARLTAVSAV
                     SLNPLEVLEAGGAGVLLEPDQEFGCVMPQLGASSIPVLMFDPPDSLPDTGRVTLRGFD
                     PSAGFEPAPRASWRVPGVPVQALPLLSSQRAAVLCHGPHGASVVVVDLRTQGLAAEAS
                     LPLPGGEPVHGAESPLEMAISADGSRLLVLTSGFSLESPEGTRRSWLHQLDAATLQLV
                     DEPLELPGVPQSPARSLWPLGNGACWIATRRPGEGFAFLHRLPLTASAPRAVEQPFTN
                     VPEALLAAPAPKGDALAVGVRNRLEIWPAGEPAGMPLQFEDELTFATWLESGLFVGEG
                     RRLHQVNPGTGVVMATIAFTTGIPAEVLPLESGAASMELPLPDGGASAIWPAVVAFRG
                     EAVGHERRVIRMRAEGQPEIPWRLEYDAAAMPWLHVHPRSGGPGQAEFAVMGVDASVS
                     TAQPPDALLRGFIRIILGDEVSLAGGPGAREHAMVVLVSPRPSGPRRILWLLGHSEGA
                     EELLISGGGHPLQAATALLAGPPHWFSHQAASSPLSEPLTEHTLVVLTARAASEGIVT
                     RQALLSCVAAGGGVLFLGSHLEESEGRALTDWLAPIGVRLDTTKAVSGEFDVSGAPLL
                     CRNWDRVAIQQGCRIEVNPPGTTLVRDPARGSAIFAVAPYGLGRFAFLASATPLENAA
                     LSGTAQRRFVGDLIRWLDSAAREVSDVDGDGIPDAIEDANQNGALDPGETDRFNPDTD
                     GDGIPDGIEDRNRNGRVDEGETSAINPDSDGDGIPDGADLSPLPAAGAPVIDAVRPAA
                     WPAEGGTWVQLSGRNFAPGMRVRFGGLPAEQVRVLGPQGLEALLPAWPQEEGGVVDVA
                     VEGLAPGPGSTIPGGFRYLPRSAVTLELGPVDITGNGEFQLALTASAGRMAQVDRLTF
                     RVNTEPPRALEWLDVLPGADALRSGRQVTSRPDPEGGLWLDISPARAGYPLGVLVRIH
                     GRPTAQPSETAPEFTITRARAFTPGGVLLEVVTGN"
     gene            complement(5491..5703)
                     /locus_tag="PKW60_05720"
     CDS             complement(5491..5703)
                     /locus_tag="PKW60_05720"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPJ98963.1"
                     /translation="MTIEYRPRGSGLYRSPNGILFGVCRGIAEYFDLSLFWLRMIVLA
                     AFILTGFFPVALAYILAALLMKRKPV"
     gene            6006..7082
                     /gene="pspF"
                     /locus_tag="PKW60_05725"
     CDS             6006..7082
                     /gene="pspF"
                     /locus_tag="PKW60_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HPJ98964.1"
                     /translation="MIEPHEGRRREDVEAAIPELVEALGQSEAFLAFQEHLSRAARVD
                     RPVLILGERGTGKELAATRLHFLSLRWQHSFVALNCAALAPSLIESELFGHEAGAFTG
                     ATARRAGRFELAHQGTLFLDEIGLIPLETQEKILRAVEYGVFERVGGSRPVHVDVRIV
                     GATNADLRTLVNAGRFRQDLLDRLSFEVLHLPPLRARQDDALLLANHFAARMAHELGW
                     DDVPEFSDEALLALENYAWPGNVRELKNVVERSVYRAGSAYVESIVFDPFAGVFAPPA
                     CPPAEPCRVPTAETSAPVPAPPAWDVDDARPYAEVLREVEIRLLRRALHRCQFNQRKA
                     AESLALTYHQFRGLYRKYQDALQE"
BASE COUNT         1282 a         2308 c         2296 g         1299 t
ORIGIN      
        1 gaccttggca gcacatggtg gccgaaatcg ttccagtcaa cgtgttcgag cagcagtccc
       61 ggcagcaggt cggcgttgaa cacgtaaatc cccatcgacc cgagatagtt gcggccttcc
      121 gagtcaaggc cgaaatcgtc gaataccgcc ttgggggtaa cgagttcctg acactgtgct
      181 tcgtccgacg gtttctcgac aaaattcacc agccgcccgt tggggtcaac ctgcatcacg
      241 ccaaacccgc gcgcggcgtc tctggcaacc ggcagcgccg cgaccgtaat ctccgcgcct
      301 ttgtcaatgt gcgtccgcag aatggcgcca tagtccatgc tgtacagctg gtcgcccgac
      361 agtatcaaaa agtatttggc gttgagattg cagaaatggc gcagctgttt acggacggca
      421 tcggccgtgc cctgatacca gtccccgctc tggtaggtct gctcggccgc caggatctcg
      481 acaaatccct gggaaaaggc atcgaatttg tacgtgtggg agatgtgccg gtgcagcgac
      541 gcgctgttga actgagtcag cagaaaaaca tggttgatct tcgaatgaat gcagttgctg
      601 atcgggatgt ccaccagccg gtaacgcccg cacagaggca ccgcgggttt ggcccgctcg
      661 gaggtcagcg gccgtaaacg gctgccgcgc ccgcccccga gaatgatggc tgtcacatcg
      721 cgtgtgatat cgaaagggtt cgaagacggg ccggtgaccg attcaggcat gttcagacct
      781 cattgtgcgc gcttgccggt gcccgcggca aacccggtat ccatgccacc cgcaagctcg
      841 cgggggtggt gcgctccggt gcgcacccgg ggggctggaa ccggcggact tcctctgttg
      901 actctatcat gacccaagcg cctgttcggg tacaagcaat ttagcgatcg gctcccgaac
      961 tcccgcagtc cgaggctttt ttcacgaatg aaacgccccg cacgcgcagc attgcgtatg
     1021 gtgtgctcga aattctacac ttcacgcatg gctgattttc tactgcgaat gctggaccgg
     1081 ctggaagcgc tcgggatccg cacacagaag ctccgctgga aactgtatca gtgggagaag
     1141 aaacgtgaag cggcgcgcga gcgaggcaca gccctgccta cccggctgca atggatgacg
     1201 tatccccaca agttctgctt caactgcaat gccctgaacg atcgggatgc gcggcgctgc
     1261 gacaaatgcg gggcgcggct gccgtcgttc ctgggataca agctgttccg cctcttcggg
     1321 atcatcacgc cccagggcgg ggcgccgacg gtgatgattt tcatggccgt catgattggc
     1381 ctgttcgggc tgagcctcct tctgggcggc ctgcagaccc tcgtggggcc cgattttaag
     1441 accctgctcc gattcggcgg ctggatgcct ttgatgatgc gggatccctt gcagtactgg
     1501 cgggctctgg cattcggcct gtatcacgcg ggcctgattc atatcgtgtt caatctgttc
     1561 gcactcagcc agattggacc tatcatggaa aatcaggtcg gccgttcgcg catgctcgtc
     1621 gtgatcaccg tgtgccagct gacggccgcg gcggggtcct ttatctggta cggcctcgcc
     1681 caaggcatgc cgctggtgcc cactctgggc gcctcgggct ggattttcgg gctgattggt
     1741 tttgggatcg cgtactttca cacgggcggc cccggcctgc gcatctaccg cgatgccctg
     1801 gtccgctggg tggtctatat gctgatcttc gggttcattg tcccgcgggt gaacaacgcg
     1861 gctcatctcg gaggcctggc gggcggcttg ctgctgggct ggttccccga ggcgcatccg
     1921 cgccggagcg ccctggtgcg ccatttctgg tcggcggctt tctggatcag cctgtttttg
     1981 tggcttgtca ccgccgtctt cctcgcatac tccatcgccg ccaatccgtt caaagagctc
     2041 tttcaatagc gccgggttat gctgcacgaa gtccgtgcgg ccaaccctga acggctctcc
     2101 cggaggcagg caggccccgg ggttcgagtg cacggaggcc gcgaaagctg cttttcagcc
     2161 cggccgtcct gctaatttcc cgttacaact tcgaggagca ccccgccggg cgtgaaagcg
     2221 cgggcgcggg tgatggtgaa ttcaggggct gtctccgatg gttgcgcggt ggggcgcccg
     2281 tgtatccgca cgagcacgcc cagcgggtag ccggcgcgtg cgggcgaaat gtcgagccag
     2341 aggccgcctt cgggatcagg gcgggaggtg acctgccgcc cggagcggag cgcgtccgcg
     2401 ccggggagaa catcgagcca ttccagcgcg cggggcggtt ccgtgttcac gcgaaacgtc
     2461 agccggtcca cctgagccat ccgtccggcg gaggccgtaa gtgcgagctg aaactctccg
     2521 ttgccggtga tgtccactgg gcccagttcg agcgtgacgg cactgcgggg gaggtagcgg
     2581 aacccgccgg gaatcgtgct gcccggcccc ggtgcaagcc cctcaacagc gacatcgacc
     2641 acgccgcctt cctcttgcgg ccacgcgggc aggagcgcct ccagaccctg gggtccaagc
     2701 acgcgcacct gctccgcagg gagcccgcca aaacgcacgc gcatgcccgg cgcaaaatta
     2761 cgtccgctga gctgcaccca ggtcccgccc tcggcaggcc atgcggcagg ccgcacggca
     2821 tcgattacgg gcgcgcccgc ggcggggagt ggcgacaagt ctgccccatc ggggatgccg
     2881 tcgccgtcgg aatccgggtt gatggcgctg gtttcaccct catccacgcg accgttccgg
     2941 ttgcgatctt caatgccgtc ggggatgccg tcgccatcgg tatcggggtt aaaccggtcg
     3001 gtttcaccgg gatccagagc gccattctgg ttcgcgtctt ctatagcatc gggaattcca
     3061 tcgccatcca cgtcggatac ctcgcgcgca gcgctgtcga gccagcggat caggtcgccc
     3121 acgaaacgcc gctgtgccgt gccagacagc gccgcatttt cgagcggcgt ggccgacgcg
     3181 agaaatgcga aacggccgag gccgtagggc gccacggcga agatcgcgct gcctcgcgca
     3241 gggtcgcgta cgagagtcgt gcccggcggg ttcacctcaa tccggcagcc ctgctgaatg
     3301 gctacccgat cccagttacg gcacagcaga ggcgctccgg acacgtcaaa ttcaccagag
     3361 acggctttcg tggtgtccag ccgcactcca atgggggcga gccaatctgt gagcgcccgg
     3421 ccctcggatt cctcaagatg acttcccaga aaaaggacgc cacccccggc ggccacacag
     3481 gacagcagcg cctggcgcgt cacgatgcct tccgaggcgg cccgggccgt cagcaccacc
     3541 agggtatgtt ccgtcagagg ctcgctcaat ggcgacgaag ctgcctgatg actgaaccag
     3601 tgcggcggac cggccagcaa cgccgttgca gcctgcaggg gatggcctcc cccggatatg
     3661 agcaattcct ccgcgccttc agaatggcct aaaagccaca aaatacggcg cggacccgac
     3721 ggccgtggcg ataccagcac caccatcgca tgctcgcggg cgcccgggcc gcctgcgagt
     3781 gatacctcgt cgcccagtat gatccggatg aatcctctca ggagtgcgtc gggcggctga
     3841 gccgtgctta ccgacgcatc aacgcccatg accgcgaatt ccgcctggcc gggaccaccg
     3901 gagcgcgggt gaacgtgcag ccagggcatg gcggctgcgt cgtattcgag ccgccagggg
     3961 atttcgggct ggccttcggc gcgcatgcgt atcacgcgcc gctcgtgacc cacggcctcc
     4021 ccgcgaaacg ccaccaccgc gggccagatg gctgacgctc cgccgtccgg gagcgggagc
     4081 tccatcgagg ccgccccgct ttcgagggga agcacttccg cggggatacc ggtcgtaaat
     4141 gcaatcgtgg ccatgaccac gcccgtgccg ggatttacct gatgcagccg gcgcccttca
     4201 cccacgaaga ggcctgactc gagccacgtg gcgaacgtca gctcgtcctc aaactgcagg
     4261 ggcatgccgg ccggctctcc cgcgggccag atctcgagcc ggttgcggac gccgaccgcg
     4321 agggcgtctc ctttcggagc tggcgccgcc aggagggctt ccggcacgtt cgtgaatggc
     4381 tgctccaccg cgcgcggggc agaggcggtc agcggaagcc ggtgtagaaa tgcaaacccc
     4441 tcgcccgggc gccgtgtagc gatccagcac gccccgttac cgagcggcca caggctccgt
     4501 gcgggcgact gcggaacgcc ggggagttcc agcggttcat ctacgagttg cagcgtggcg
     4561 gcatccaact ggtgcagcca ggagcgccgg gtgccttccg gggattccag agagaatccg
     4621 gacgtaagca ccagcaaacg cgaaccatcg gcggaaatcg ccatttccag gggtgattcg
     4681 gcgccgtgca ccggttcccc ccccggcaac ggcagggacg cttccgcggc aaggccttgt
     4741 gtccgcagat caacgactac cacagatgcg ccatgcggcc catggcaaag cacggcggcg
     4801 cgctggctcg ataacagcgg caaggcctga accggcacgc ccggcacccg ccatgaggcc
     4861 cgtggcgcag gttcgaagcc cgcggacgga tcaaatccgc gaagcgttac tcgacccgta
     4921 tccggcaacg agtccggggg gtcaaacatc agaacgggga tcgacgaagc gcccagctga
     4981 ggcatcacgc agccgaattc ctgatccggc tccagcagca cccccgcccc gccggcctcg
     5041 agcacctcca gcggattcag cgacacggcg ctgaccgcag tcagccgggc aggccatcct
     5101 tcgggatgag gccagagctt ccgcgtggcg acgaacgcac agacgttgtc gcggcagatg
     5161 gtcagccggg aggcgcattc catgcccgga agcgtggcgc gccacggaaa aatatcgccc
     5221 tgggccatgt ccaccacgtg cagggtctgc cgcgtttcct ggcgcgatgc agtccgggcc
     5281 gttgtccgca cgagcagatg ctgcgccgac gcctcggcca tcagggctgt aaccccgagg
     5341 gcgactgcca tgtggatacc gcgtttcact ggattccctg cgttcgtgcc atccggcacg
     5401 ccagggccat tgtatcaggt gccacgccgc cggcgcgccc gggacagtgg ctttttgaga
     5461 ccggcgcaag ggtgcgcgag ccccgcgggt ttataccggc ttccgtttca tcagcaaagc
     5521 ggcgaggatg taggcaagag ccaccggaaa aaaaccggtc aggatgaacg ctgccagcac
     5581 gatcatccgg agccagaaca gcgacagatc gaaatattcg gcaatgcccc ggcacacgcc
     5641 aaacaggatg ccgttgggcg atcggtacag gccagaacct cgagggcggt attcaatcgt
     5701 cattgccgtt ttctctctgt gcggttgcat gcagccggcc cgggcgagcc ggcggatgtt
     5761 ttgaatattt cgacttcatg tctgggcccg ttatgccgcc cgtgccggtg acgacacaca
     5821 gtataagcct ccctggaggc ctggattggt ttcaggattg caaatagctt gccaaaagac
     5881 gccctgcgcc ataactgata cgattttaac ggtttatgtt ggttgtttca acacttccgc
     5941 ttgacggatt tggcgaaaat cgctattatt tggcgaaaat agtcaaaaat gaagggtttt
     6001 aagccatgat agagcctcat gaaggccggc ggcgagaaga tgtcgaggca gccatccccg
     6061 agcttgtgga ggcgctgggg cagtcggagg cgtttctagc gtttcaggaa cacttgtcgc
     6121 gcgcggcccg ggtggatcga ccggtactca tcctcggtga gcgcggcacg ggaaaggaac
     6181 tcgccgcgac acggcttcat ttcctgtccc tgcgctggca acactccttt gtggccctga
     6241 attgcgccgc actcgccccc tcgcttatcg aatccgagtt attcgggcat gaagccggcg
     6301 cgtttacggg cgccacggcg cgccgggcgg ggcgcttcga actggcccat cagggcaccc
     6361 tgtttctgga cgaaatcggc ctcattccgc ttgaaacgca ggaaaaaatc ctgcgcgccg
     6421 tcgagtatgg cgtatttgaa cgggtcggcg gctcgcggcc ggtccacgtg gatgtgcgga
     6481 ttgtcggcgc caccaacgcc gatctgcgca cgctggttaa cgcggggcgg ttccggcagg
     6541 acctgctcga tcgtttgtcg tttgaagtgc tccatttgcc gccgctgcgg gcgcgccagg
     6601 acgacgccct gctgctggcg aaccattttg ccgcgcggat ggcccatgaa ctgggctggg
     6661 acgatgtgcc cgaattcagc gacgaagccc ttctggcgct cgaaaattac gcatggccgg
     6721 gaaacgtgcg cgaactcaaa aacgtggtcg agcgcagcgt ctatcgcgcg ggctccgcgt
     6781 atgtagaatc cattgtgttc gatccgtttg cgggcgtgtt tgcgcccccc gcctgtcctc
     6841 cggcggagcc atgccgtgtg ccgactgccg agacatccgc cccggttccc gcacccccag
     6901 cctgggacgt ggacgacgcg cggccctacg cggaggtgct ccgtgaggtc gagatccgcc
     6961 tgttgcgccg cgcgctccac cgttgccagt tcaaccagag gaaagccgcg gagagcctcg
     7021 cgctcacgta tcaccagttt cgcggcctgt accgcaaata ccaggatgcg ctccaggagt
     7081 gaccggcccg gtcctccgga ggcggcatgc aggcggaaca ggtccttttc agaatattcg
     7141 acagcagcat ttgtgccgtg ggcagtgtat aatacggcct ttgcg
//
LOCUS       DAOPBQ010000129         7183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9346_length_7183_cov_4.158530, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000129 DAOPBQ010000000
VERSION     DAOPBQ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9346_length_7183_cov_4.158530"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="PKW60_05730"
     CDS             <1..994
                     /locus_tag="PKW60_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPJ98965.1"
                     /translation="AFRSLLPSGAKQPRFLNGAAQDLDGDGIDEWLIPTNGGYLIRNA
                     TEQRCLIPCEAESDIRDNGTIQIKHQFPIVHAFPLENQTLKGLAFLNKKRADFAYGDN
                     WTQRQRYSVPLDSEEKWDVSVQMQDIDGNGLPDLIVSLSKGTVNVTAMTHVYIASAPF
                     TYPETPSAVFNADGAIAAPYLIDVDGDGKLDVLFIKVPFGVKTVLNLFLRKKIAVEVD
                     VFLFNGTGYNAKPDFGTTITIDAPEQREVPVYTMADFSGDGRMDVMFGSGNSDIVIHR
                     GETSRFISHKPWVTLPFAPFGEARPCDLNANTAKDLVLFHPNGKDSKRIEVAVF"
     gene            1201..3600
                     /locus_tag="PKW60_05735"
     CDS             1201..3600
                     /locus_tag="PKW60_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPJ98966.1"
                     /translation="MRQIHWYAILARACFVTALVVIAAPAAEAQGPRPARHPLLEIEP
                     VPSGVGVNIHFYAGNEHDLRMLEECGPGIIRMDISRAGAEKSPGAYDFSHYDRLVADM
                     EARGIRILFIIDYGSPLYDDGLAPRSEGGRAAYARFCSALAAHFAGKPIIWELWNEPN
                     LDFWKPEPNVEDYLAWCKVVVPAIREADPGACIVGPATSGFPWEFLQTCFENRYLDLV
                     DGVSVHPYRGSFEGPETARRDYDRLAAMIDRYKTAQSNTRDIPILSGEWGYTTTNIPR
                     DLQGVFLPRQWLSNLAHGIPISIWYDWHDDGQDPDEREHNFGTVTWDYQPKPAYTAMK
                     TLIAELKGYTPAGTLPMGRGSDFVAVFRKDSSYKLAIWTTEESSELTLPEGLNVIRAI
                     DHHGQPVDVPPDGVWRVSGAPVYLTLSPEAPPWLGILPLQPGPIHPAKAGTQLDVSIP
                     FSATCSAPDGLELSGEVTSIPGIEGHWLPNSASLVPGTPAQLIWKGTITRRDAAEVSV
                     PVAITCRGREAGTHTFRMNVRVPVANPIKMTLGWRHDGLCVELHSQSDLPLTGQLLVK
                     AGVQPLPAVPVELKGNEIRQIPFPNLTLSDGPQTVSAQMLHENAGIIAEVTPLTYVLV
                     DAIDEPAGLDAREAYRLWDEGEENREITLAGEVIESPGDAPPFPKSVRVGYAVEPGWC
                     FWQWGPQKNLELPASRPSKMMLWVHGPAFTDHMLCRIVDAAGQSFKTAPVSKARDGWE
                     LIELSLDKPVAHWGGAGDGVMHPPFRWTSYYLQESREARSGTTCLTGVVLAWAPEPAE
                     E"
     gene            3654..7055
                     /locus_tag="PKW60_05740"
     CDS             3654..7055
                     /locus_tag="PKW60_05740"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HPJ98967.1"
                     /translation="MRNALFWTFLAVCCCIAVAQPSTVELPPGETALGDLGLYQVAWQ
                     SYGHDAVLMPPSWTGHFHRVSGISYVPHELMLGRDAILLHSPWHVPPGCAWVDYPLRL
                     PEMSPITLSFGIAMSPLAAEPDKSDGVTFSAIILQDGQEETLLREHYAMAEWKDFSFD
                     LTPYAGQALTLRLQADPGPANSPAWDYSYFGDAKIVCGSRSAGEKDLLARLVSAKAFQ
                     AARGRDISPLCNDPGNGITPSNLLEHENTVVKTDRGYRFEYTGADGHLSYEYAPQTGT
                     LDDFTVQFDEGPPFQPAMGGGIMIQAPDADAPPFRLGQGRLLSASLDETARRLTVNWA
                     YPAGGATFEASWTFGIAGKALTIAVDAEMPIVTDFSLGYDGGVPFRQAFYVPYLAGTV
                     SYLRGQQVFTCRYLDWTRSHASRCPQGEAEYDLMTNQTRNPLHEEGYIAVSANIQEVL
                     PNIPHPPSPYRDLLGPRIMLDIWGHHRGSFAGDGENLRDLKDNGVDHLAIISHVWQRY
                     GYDVKLPDHLPPNPKYGGREGMELFAKAANECGYVWSLHENYIDLYPDAPSHDPADRV
                     LLTDGSPSPAWLNPATKVQSFGIKCNRALKFAKENAPLIHEWFQTNAAYLDVHTCVPP
                     WHQLDHEAGQPMAGMILAKVKYDSALFQYMRDTHEGPLLGEGANHFYWAGLCDGVEAQ
                     VSGGEDHRPFLDFDLLKIHPQMVNHGMGYYERWFRRGYGHRWGHDTGTVEQIDKYRAQ
                     EVAYGHAGFIGNAQTNNVQWVAKEHHMMHPIQRLYGAANPVEVAYAFDDDFYPASIAL
                     VMGRTDRQRIRYDSGLTVWVNWADDDWTVEGHTLPQWGFLALGPETRVATYRINGHFA
                     DYAECPEFVFVDARTEFSMPYWKRPLKIEPRLKSLDYQGEGRIRVAYEWIVGEDVNQD
                     YRCFVHFTSDSSDHIERTAFQQDHTLPKPAHEWRRGDVITDGPYEVVIPDSPDESFDI
                     RIGLTNESRVPLEGFDDGARRYRIGAMHIQRENGAIVGVSLDDLERERAGYLSSKADF
                     DAHMNPAGTWIDFGKLATDGSVKINKEENRLIVFPYPRDRAFGLEIDLASFGAKAGAD
                     NVRVMALKAGSQESMGSVPFELQGSRIRFQTGLIGCGRYIVEWGS"
BASE COUNT         1488 a         2156 c         2147 g         1392 t
ORIGIN      
        1 cgcgtttcgt tccctgctgc ccagcggcgc aaagcagcca cgtttcctga atggggcggc
       61 tcaggatctt gacggtgacg gcatcgatga atggctgatt cctaccaacg ggggttacct
      121 cattcgcaac gccacggagc agcgctgcct gattccctgc gaggcagaaa gcgacatccg
      181 cgacaacggg acaattcaga ttaagcacca gtttcccatc gtgcacgcat tcccgctcga
      241 aaaccagacc cttaagggac tcgcattctt aaacaaaaaa cgcgccgact tcgcctacgg
      301 cgacaactgg acccaacggc agcgctatag tgtcccgctg gactccgaag agaaatggga
      361 cgtctccgtt cagatgcagg acattgatgg gaacgggttg cccgacctca tagtcagcct
      421 gtccaagggc accgtgaatg tgaccgcgat gacgcatgta tatatcgcat ctgcgccatt
      481 cacgtatcca gaaacgccca gcgcggtgtt caacgcagat ggcgccattg ccgcaccgta
      541 tctgattgac gttgacggcg acggaaagct cgacgtcctc tttatcaagg tgccatttgg
      601 cgttaaaacg gtcctgaatc tcttcttaag aaagaagatc gccgtagagg tcgatgtctt
      661 tctgtttaac ggcaccggct acaacgccaa acccgacttc ggcacgacca tcacgatcga
      721 cgcccccgag cagcgcgaag tgcccgtcta taccatggcc gatttcagtg gcgacgggcg
      781 gatggacgtg atgttcggtt ctggaaactc cgacatcgtc atccatcggg gcgaaacctc
      841 gcggttcatt tcccacaagc cgtgggtcac tctcccgttt gcgcccttcg gcgaggcgcg
      901 cccctgcgac ctcaacgcca ataccgccaa agatcttgtc ctgtttcacc cgaacggcaa
      961 agacagcaaa agaatcgaag tcgcagtatt ctgaacggcc cgtgcgggca cgtactcgta
     1021 aaccgaagta catgtatcgt attctggctg ttgtttcagc cgcgcctggc gcgctcctcc
     1081 gggatgggcc gtccccgttc gtgcgcgcgg ccggggctct catctgaatc ggacgcaggc
     1141 acgaatactc tgtttgcgtg ccgtgatgaa gccggggtgt aatcggagaa atgggaagtc
     1201 atgcgacaaa tccactggta tgccattctc gccagggcgt gcttcgtaac agcgctcgtt
     1261 gttatcgcgg cgccggccgc cgaagcgcag ggtccccgac ccgcgcgcca tcctcttctt
     1321 gagatcgaac ctgtaccctc gggcgtcggc gtcaatatcc atttctacgc gggcaacgag
     1381 cacgacctgc gcatgcttga ggaatgcggg ccaggcatca tccgcatgga catttcccgg
     1441 gcgggggcgg agaaatcgcc gggggcatac gatttcagcc actatgaccg gcttgttgcc
     1501 gacatggaag cgcggggaat ccggatcctg ttcatcatcg actacggcag cccgttgtac
     1561 gacgatggac tggcgccccg cagcgaggga ggccgggcgg cgtatgcgcg gttctgctca
     1621 gcgctggccg cacacttcgc cggcaaaccc atcatctggg agttgtggaa cgaaccgaac
     1681 ctcgatttct ggaagcctga gcccaacgtc gaggattacc tggcctggtg caaggtggtg
     1741 gtgccggcga tacgagaggc cgatcccggc gcgtgcattg tcgggccggc cacctcgggt
     1801 ttcccctggg aatttctgca gacctgtttt gagaaccggt atctcgactt ggtggacggc
     1861 gtcagtgtgc acccctaccg aggttcattc gagggccccg aaaccgcacg gcgagactat
     1921 gatcggcttg ccgcgatgat cgatcgatac aagactgcgc agtcaaacac gcgtgacatc
     1981 cccattctca gcggcgaatg gggatacacg acaacgaaca tcccgcgcga cctccagggc
     2041 gttttccttc cgcgccaatg gctcagcaat ctcgcacacg gcatccccat cagcatatgg
     2101 tacgactggc acgacgacgg acaggatccc gacgagcgcg agcacaattt cggaaccgtc
     2161 acctgggatt accagcccaa accggcctac accgccatga agacactgat cgccgagttg
     2221 aagggctata cccccgcggg cacgttgcca atggggcgtg gcagcgattt tgtggcggtg
     2281 tttcgaaagg attcctcgta caagctcgcg atctggacga ctgaagagtc cagtgaactc
     2341 accttgccgg agggcttgaa cgttatccgg gcgatcgatc accacggtca gcctgttgac
     2401 gtgcccccgg acggagtctg gcgggtgagc ggcgcgcctg tctacctcac cctttctccg
     2461 gaggcgccgc cgtggctggg catccttccg ctgcagccgg ggcctataca cccggctaaa
     2521 gcgggcacac agctggatgt ctccatcccc ttttctgcca catgctccgc gccggacggg
     2581 ttggagttgt ctggcgaggt gacttctatt ccgggcatag aggggcactg gctccccaac
     2641 agcgcctcgc tcgtgccggg aacacctgcg caattgatct ggaagggaac catcacgcgc
     2701 cgcgatgcgg ccgaagtcag cgtgcctgtc gcgattacct gccgtggccg cgaggccggt
     2761 acacacacct ttcgcatgaa cgtcagggtg cctgtcgcca atcccataaa aatgacactt
     2821 ggatggcggc acgacggcct gtgcgttgaa ctgcattcgc agtctgatct gcctctgacc
     2881 ggccagctgc tggtgaaagc cggcgtgcaa ccactgccgg ctgtcccggt agaactcaaa
     2941 ggaaacgaga tccgccagat tccttttcca aacctcacct tgtccgatgg gcctcagacg
     3001 gtctctgcgc aaatgctgca tgaaaatgcg gggatcatcg ctgaggtcac gccactgact
     3061 tatgtgctgg tcgatgccat agacgagccc gcaggattgg atgcacgaga agcctaccgc
     3121 ctttgggatg aaggcgagga aaaccgcgaa ataaccctcg cgggcgaggt catcgaatcg
     3181 ccaggcgacg cgccgccgtt tcccaaatcg gtcagggtcg gctacgctgt ggaacccggc
     3241 tggtgttttt ggcagtgggg acctcaaaaa aatctcgagc ttcccgcatc ccgtccgagc
     3301 aagatgatgc tctgggttca cgggcccgca ttcaccgacc atatgctttg ccggatcgtc
     3361 gatgccgcag gacaaagctt caagacggcg cccgtgtcaa aggccaggga cggatgggag
     3421 ctgatcgaac tgagtctgga caaaccggtt gctcattggg gcggtgcggg tgatggcgtg
     3481 atgcatccgc cgttccgatg gacttcgtat tatcttcagg aatcgcgaga ggcgagatcg
     3541 ggcacgacct gcctcacggg cgtggtgctg gcctgggcgc cggaaccggc ggaggaatga
     3601 ttgcgcctgc gcgccgcctg taacgcatgt caactcttca ggagaaagat gtcgtgagaa
     3661 acgcattatt ctggacattt cttgcggtat gttgctgcat cgccgttgcc cagccctcca
     3721 ctgttgaact gccgcccgga gagactgccc tgggggatct cgggctctat caggtggcct
     3781 ggcaatccta cgggcacgat gccgtgctca tgcccccatc ctggacggga catttccatc
     3841 gggtgagcgg catctcctac gtgccgcacg aattgatgct gggacgcgat gcgatcctcc
     3901 tgcattcccc gtggcatgtg ccgcccggct gcgcctgggt agactatccc cttcgtctcc
     3961 ctgaaatgtc gcccatcacg ctgtctttcg gcatcgcgat gagccccctg gctgcggaac
     4021 cggacaagag cgacggcgtt acgttcagcg ccattatcct gcaggatggc caggaagaga
     4081 ccctgctgcg cgagcattat gcgatggcgg aatggaagga tttcagcttc gatctgacgc
     4141 cgtatgctgg acaggccctc accttgcgtc tccaggccga cccgggaccc gccaacagcc
     4201 cggcgtggga ttattcgtat tttggcgacg caaaaatagt ctgcgggagc cggagcgccg
     4261 gtgagaaaga cctgctggcc cggctggttt cagcaaaagc cttccaggcg gcgcgcggcc
     4321 gcgacatctc tcccctgtgc aatgatcccg gcaacggcat cacgccttca aacctgctcg
     4381 aacatgagaa tacagtcgta aagacagaca ggggctaccg gttcgaatac acaggcgccg
     4441 acgggcatct ttcgtacgaa tatgctccgc aaaccggtac gctggatgat ttcacggtcc
     4501 aattcgacga aggacctccg tttcaacccg cgatgggcgg aggcatcatg attcaggcgc
     4561 cggacgctga cgcgccgccg tttcgcctgg gacaagggcg cctgctcagt gcatcactcg
     4621 acgagacagc ccgcagactc accgtaaact gggcctatcc cgcgggaggc gccacctttg
     4681 aggcaagctg gacgttcggc atcgcaggaa aggccctgac cattgctgtg gatgcggaaa
     4741 tgcccatcgt gacggacttc tcgctcggat acgacggcgg cgtgccgttc cggcaagcgt
     4801 tctatgttcc gtaccttgcc ggcaccgtgt cgtacctgag aggccagcag gtgttcacgt
     4861 gccgctacct tgactggacg cgctcccacg cgtcgcgatg cccgcaaggc gaggccgaat
     4921 acgacctcat gacgaatcag acccgcaatc ctctgcacga agagggctac atcgctgtat
     4981 ctgcgaacat ccaggaagtt ctgcccaata tcccgcatcc accgtctccg taccgggacc
     5041 tcctgggccc ccggatcatg ctggacatct gggggcatca ccggggatca tttgcgggag
     5101 acggcgaaaa tctgcgcgat ttgaaagaca atggcgtgga tcatctggcc atcatttccc
     5161 acgtgtggca gcggtatggc tacgacgtga agctgccgga ccatctgccc ccaaacccga
     5221 aatatggcgg acgcgaaggc atggaactgt tcgccaaggc agccaacgaa tgcgggtatg
     5281 tgtggtcgct gcacgaaaac tatattgatc tctatcccga cgcgccctcc cacgacccgg
     5341 cggatcgcgt gcttttgacg gacggatcgc catcgcccgc ctggctcaat ccagcgacaa
     5401 aagtgcagag ttttggaatc aagtgcaacc gggcgctcaa atttgctaaa gaaaatgccc
     5461 cgttgattca cgaatggttc cagaccaacg ccgcctacct ggatgtccac acctgcgtac
     5521 cgccctggca tcagctggat cacgaagccg gccagccgat ggcgggcatg atactcgcca
     5581 aggtcaagta cgactcagca ttgttccagt acatgcgcga cacacacgaa gggccgttgt
     5641 tgggcgaagg cgcgaaccac ttctactggg ccgggctctg cgacggcgtt gaggcccagg
     5701 tcagcggcgg cgaggaccac cggcctttcc tcgattttga tttgctgaag atccatcccc
     5761 agatggtcaa tcacggcatg ggatactacg aacggtggtt tcgccgtgga tatgggcatc
     5821 gctggggcca tgatacaggg acagtcgagc agattgacaa ataccgcgcc caggaagtgg
     5881 cgtacggcca tgccgggttc attggcaacg cgcaaaccaa taacgtccaa tgggtggcca
     5941 aagaacacca tatgatgcat ccgatccagc gtttgtacgg cgccgcgaac ccggttgagg
     6001 tcgcctacgc ctttgatgac gatttttatc ccgcaagcat cgccctggtg atgggccgta
     6061 cggaccgcca gcgcatccgg tacgacagcg gcctcaccgt ctgggtcaac tgggctgacg
     6121 atgactggac cgtggaaggc catacgctgc cgcagtgggg atttctggcg cttggtcctg
     6181 agacacgcgt ggcaacatac cggatcaacg gccattttgc cgactatgcg gagtgccccg
     6241 aattcgtgtt cgtggatgcg cgcaccgaat tctcaatgcc ctactggaag aggccactga
     6301 aaatcgagcc gcgcctgaaa tcgctggatt accagggtga aggcaggata cgagtggcct
     6361 acgaatggat cgtgggcgaa gacgtgaatc aagactaccg gtgctttgtg catttcacga
     6421 gcgacagcag cgatcacatc gagcgaaccg cgttccaaca ggaccatacc ctgccgaaac
     6481 ccgcccacga gtggcggcgg ggcgatgtca tcacggacgg cccgtatgaa gtggtgatac
     6541 cagacagtcc ggacgagagc ttcgacattc ggatcggtct gaccaacgag agccgggttc
     6601 cactggaggg attcgatgac ggtgcccgcc gctaccgcat cggcgccatg catattcagc
     6661 gggagaacgg cgcgatcgtt ggagtgtccc tggatgacct cgagcgggag cgggcgggtt
     6721 acctgtccag caaggccgat ttcgatgcgc acatgaaccc cgcgggaacc tggatcgatt
     6781 ttggcaaact ggcgacagat ggctcggtca aaatcaacaa agaagagaac cggctcatcg
     6841 tatttccgta tccgcgcgac agagcattcg gactcgaaat cgaccttgcc tcgttcggcg
     6901 ccaaggccgg cgccgacaac gtccgtgtga tggcgctgaa agccggctca caagaaagca
     6961 tggggtcggt gccctttgag ctgcaaggca gccgtatcag attccagacc ggactgatcg
     7021 ggtgcgggcg gtatattgtg gagtggggat catgacatgt gcccggtttt gcgatacgcg
     7081 ggaaggagct ctgtgatgcg aagtattctc gtgcggatgg ggcccatcgc cgggatgtgc
     7141 atgttcctgg cggcaattgc cggtgcggaa acaccggtgt ttt
//
LOCUS       DAOPBQ010000130         7163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9385_length_7163_cov_3.647440, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000130 DAOPBQ010000000
VERSION     DAOPBQ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9385_length_7163_cov_3.647440"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..2113)
                     /locus_tag="PKW60_05745"
     CDS             complement(302..2113)
                     /locus_tag="PKW60_05745"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HPJ98968.1"
                     /translation="MSKKDVSAGNNRWIGDFPKIGIRPIIDGRRKGVRESLENQTMSL
                     AKAAARLLTRQLRYPNGAPVECVIADTCIGGVAEAAQAAAKFAKEGVGVSLSVTPCWC
                     YGSETMDMDPLMPKAVWGFNGTERPGAVYLAAVLAAHNQKGLPAFSIYGRDVQDSGDT
                     AIPADVQEKMLRFARAGLAAALMRGKSYVSMGNVSMGIAGSVVDQSFFESYLGMRVET
                     IDMSEFVRRIDENIYDKAEFEKGLKWVKKHCREGKDGNPKHLRLSPAQKAKVWETCVK
                     MYLIGRDIMCGNPKLARMGWGEEALGRNALAAGFQGQRQWTDHFPNGDFMEALLNSSF
                     DWNGIRAPYLMATENDCLNATAMIFGYLLTNTAQVFADVRTYWSPDAVKRVTGHVLSG
                     DAAGGLIHLINSGAAALDGSGRQTHKGKPVMKPFWEVDAKEVKACLEATTWYPGNREY
                     FRGGGFSSCFLTEGGMPVTLIRVNLVKGLGPVLQLAEGVTCSLPYEVHAQLNDRTDPG
                     WPTTWFAPRVTGSGPFRDVYSVMNNWGANHGSFAYGHIGADLLALASILRIPVCMHNV
                     EEADVFRPSAWSAFGAQDPMGADYRACANFGPLYGRY"
     gene            complement(2286..3296)
                     /locus_tag="PKW60_05750"
     CDS             complement(2286..3296)
                     /locus_tag="PKW60_05750"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPJ98969.1"
                     /translation="MEGQHTFLTWSDDHGASWSAPTAPFDPPVLNGRTGRFRALFCTP
                     LGGRRVLATLYWVDASNPERSFFNEATEGLLDSHIFHALSEDAGATWASPVKMDTTPY
                     DQPTPITGPALLMPDGRWVCQFELNKPYTDPTPWRHAPVLMFSRDAGLTWPESVEVCP
                     DPANLVFYWDQRPSVLPDGSLFDVFWTYDRAAGAYRNIHATRSVDGGRSWQPAWDTGV
                     PGQPAPVIALENGVLALVYMDRTGTPALKLRLSRDGGHTWPQCSERTLDDFSALRPGG
                     HATMQDAWAEMGAFSSGLPAACRTLDGDLLVVYYAGPHTDHTDIHWMRLDGAALAAEG
                     VV"
     gene            complement(3440..4558)
                     /locus_tag="PKW60_05755"
     CDS             complement(3440..4558)
                     /locus_tag="PKW60_05755"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPJ98970.1"
                     /translation="MIWYALVPVVLALSAPGVAADGPIELLGSMERWAQSGGSEGSFV
                     LDAGTLSLKKAEGLPGELLTAADYENFELRFEFNVASWTATGLYLHAPRNRQYPAGLE
                     LELSGHPTEHDTPYTTGAIFRRIPPRVNARKKPGEWNTCEIHMDWPHLHVTMNGQIVQ
                     DVDLEAAGLGYTLRRGAIGFQNSGQDALQVRNMTIIPLPDSEHGIVLPTDEALTGWTV
                     ANGEATWAAADGVLRGADGNGHLVHEAVCTDFDFRCYFRSSAATTNGGIHFRWPEDNS
                     HSGQEVQIYDVPAAVMPTGSIYGIARCDDALIRPGEWMLVQIIARGSHVTTYIDGRKA
                     AESFYFVTVRNPGRMALQMHSAGWIEFKDLVLVPKDTR"
     gene            4974..5114
                     /locus_tag="PKW60_05760"
     CDS             4974..5114
                     /locus_tag="PKW60_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98971.1"
                     /translation="MKHLKTLTRRPVEAADIPTLINIVALIQGLLTAILSFTQAKEGA
                     GT"
     gene            5527..6063
                     /locus_tag="PKW60_05765"
     CDS             5527..6063
                     /locus_tag="PKW60_05765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPJ98972.1"
                     /translation="MIFGEHCCIRAAEPDDAPALKRVYQRAKPRAAALDRRREPIIPT
                     TDELRELLSSKDVKLLGEYYAVEDATGEIRGFCCLRAGRQDVFYGEMLLLFIEDADLD
                     TGMAGETFAFLKNKAFVERRLNKIVTQCLDTETALRSFLLRQGFTSDGIAREVLYTLG
                     RWFNNETLSLRASQHEDG"
     gene            6067..6696
                     /locus_tag="PKW60_05770"
     CDS             6067..6696
                     /locus_tag="PKW60_05770"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPJ98973.1"
                     /translation="MALRSNIFIRRAEREDLDTVVEWMEDPDFQLFLYGDVTRSQRQI
                     REQIVGLLGRAPGHTMPAAFQLILDSEADGPVGLVLLQNVSWRNRSCSLDVYVGQKNL
                     RKGIVIGLAFYRIMEYCFDELNLHRVSAYIYSFNTPSWRIMERSNAKRELTLKDHVVR
                     NGELHDMYGYGLLRREFDELRESLKGRMEGKSLLEMIEALHAKEQGAGA"
     gene            6693..>7163
                     /locus_tag="PKW60_05775"
     CDS             6693..>7163
                     /locus_tag="PKW60_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98974.1"
                     /translation="MRIALLAFLGLHVAHALWLNGRQDRVSSNAENRASLYLVQVPLF
                     VLGCIIAWQNGDVGRGLVNPLTIAAGLLLGHVIFVLSLLLVHRSARDAREVLLGLRTI
                     WEFVIESPLILSRYVGVAVTEEVIYRAAAQRILCTYLRSSWAGMVIVAVLFSVVH"
BASE COUNT         1362 a         2075 c         2317 g         1409 t
ORIGIN      
        1 gggattcgct gctcagataa tcgaggatcg acatgcccgc ccagttgggc agccaggggc
       61 tgtcgatgat gaaccccgcg ggcggcgggc cagcatgggt gccgtccacc acggccagca
      121 gggtgtgcca ttgctcctgg gtcatgggat gaaactcact ccagcaatcc tcggcatttt
      181 gtgtgcttct ccggcccggg ccaggcagat cccgccacaa gggaacgata cgccctcctc
      241 agaagaaaca acgcggggga atctcccagg cgggagactc ccccagcggc tacaccggtt
      301 tttagtagcg gccgtacaga ggcccaaaat tggcgcaggc gcggtagtcg gcgcccatcg
      361 ggtcctgtgc accaaacgcc gaccaggccg agggacggaa cacgtccgcc tcttcgacgt
      421 tgtgcatgca gacgggaatg cgcaggatcg acgccagcgc gagcaggtcg gcgccaatgt
      481 ggccgtaggc gaatgacccg tggttggcgc cccagttgtt catgaccgaa tagacatcgc
      541 ggaacggtcc cgaccccgtg acgcgcggcg cgaaccacgt ggtgggccag ccgggatcgg
      601 tccggtcgtt gagttgggcg tgcacctcgt acgggaggct gcaggtgacg ccttcggcga
      661 gctggagcac cggcccgagg cctttgacca ggttgacgcg aatgagcgtc acgggcatgc
      721 cgccctcggt caggaagcac gaggagaaac cgccaccgcg gaagtattcg cggttgccgg
      781 ggtaccacgt ggtggcttcc aggcaggctt tcacttcctt ggcgtcgacc tcccagaagg
      841 gtttcatgac gggtttgccc ttgtgggtct gtcggccgct gccgtcgagg gcggccgcgc
      901 ccgaattgat gaggtggatc aggccgccgg cagcgtcgcc cgacagcaca tggccggtga
      961 cgcgcttgac cgcatcgggg ctccaataag tccgcacgtc ggcgaagacc tgcgcggtat
     1021 tcgtgagcag gtagccgaag atcatggccg tcgcgttgag gcaatcgttt tcggtggcca
     1081 tgagataagg cgcgcggatg ccgttccagt caaacgagga gttcagcagg gcttccatga
     1141 aatcgccgtt gggaaaatgg tcggtccact ggcgctggcc ctggaatccc gcggccaggg
     1201 cattgcgtcc gagggcttct tcgccccagc ccatgcgcgc aagcttgggg ttgccgcaca
     1261 tgatgtcgcg cccgatgaga tacatcttca cgcaggtttc ccagactttg gccttctggg
     1321 cggggctcag gcgcaggtgc ttcgggttgc cgtcttttcc ttccctgcag tgcttcttca
     1381 cccacttcag gcctttttcg aactcggctt tgtcgtagat attttcgtcg atgcggcgca
     1441 caaactccga catatcgatg gtttcgacgc gcatgccgag atagctttcg aagaagctct
     1501 ggtcgaccac cgagcctgca atgcccatgg agacgttgcc catcgacaca taggattttc
     1561 cgcgcatgag ggcggcagcg aggccggcgc gcgcgaagcg cagcatcttc tcctgcacgt
     1621 cggcgggaat ggcggtatcg cccgaatcct gcacgtcgcg gccatagatg ctgaacgcgg
     1681 gcaggccctt ctggttgtgc gccgccagca ccgcggccag atagacggcg ccggggcgtt
     1741 cggtgccatt gaagccccag acggctttcg gcatgagggg atccatgtcc atggtctcgg
     1801 aaccgtaaca ccagcagggc gtgacggaga gcgagacgcc cacgccttct ttggcgaact
     1861 tggcggcagc ctgagcggct tccgcaacgc cgccgatgca ggtgtccgcg atgacacatt
     1921 cgacgggggc gccgttcgga tagcgcagtt gcctggtcag gagtctggcc gcggctttgg
     1981 ccaggctcat ggtctggttt tcgagggact ctcggacccc tttacggcgc ccgtcgataa
     2041 tcgggcggat accgattttc gggaaatcgc caatccaacg attgttgcct gctgagacat
     2101 ccttcttact catgatttcc tccacttaat tccgattggc gttgtacgcg cagtatcttc
     2161 cccagtatat aaacggaaac gcgccagaac catggcgatc attcttcctt cacgttacct
     2221 gttggacagc gggaaggcaa gttccgcgcc gcggtgcgcc aaggtgaaac gcccgtgtcc
     2281 gtggctcaga caaccccttc cgctgcaagc gccgccccgt ccagccgcat ccagtggatg
     2341 tcggtgtgat cggtgtgcgg cccggcataa taaaccacca gcagatcgcc gtccagcgtg
     2401 cggcaggccg caggcaggcc ggatgaaaag gcgcccatct cagcccaggc atcctgcatg
     2461 gtggcgtggc cgcccgggcg caaggctgaa aaatcatcaa gcgtccgctc gctgcattgc
     2521 ggccaggtgt gccccccgtc ccggctcagg cgcagtttta gcgccggcgt tccggtccga
     2581 tccatataca ccagcgccag gacgccgttt tccagcgcga tgaccggtgc gggctggccg
     2641 gggactcctg tgtcccatgc cggctgccag gaacggcctc cgtcgacgga ccgggtcgca
     2701 tggatgtttc ggtaggcgcc tgcggcgcgg tcataggtcc agaaaacgtc aaacaacgaa
     2761 ccatccggca gcacagaggg ccgttgatcc cagtaaaaca ccaggttggc gggatcaggg
     2821 cagacctcga cactctccgg ccaggtcagc cccgcatcgc gggagaacat caggaccggc
     2881 gcatggcgcc agggcgtggg atccgtgtac ggcttgttca attcaaactg gcagacccat
     2941 cgcccgtcgg gcatgagcag ggctggcccc gtgattggcg tgggctggtc atagggtgtg
     3001 gtgtccatct tcacgggcga ggcccacgtg gccccggcat cttcagagag ggcatggaaa
     3061 atatgactgt ccaacagccc ctcggtggcc tcattgaaga agctgcgttc cggattggat
     3121 gcatccaccc agtacagcgt tgccaggacg cggcgcccgc cgagcggagt acagaagagc
     3181 gcgcggaacc gcccagtccg tccattgagg acaggagggt cgaacggagc ggtgggagcg
     3241 ctccatgacg cgccgtggtc gtccgaccag gtcaggaagg tatgctggcc ttccacactg
     3301 tctttcgtta cagcgccgcg gaatccgcac aaccatcgtc cggaaggaag cacgcagatc
     3361 cccggaaagg cgcggctcgg gtatccggcc ggcccgcctg atgtgacccg gccctgcgct
     3421 ttgattggag cgccgccgtt catcgggtgt ctttcggcac gaggaccaga tccttgaatt
     3481 cgatccagcc cgcactgtgc atttgaaggg ccatacggcc gggatttcga accgtcacaa
     3541 agtagaacga ttccgcggcc tttctcccgt cgatatacgt ggtcacgtga gacccccggg
     3601 cgatgatctg caccagcatc cattctcccg gacggatgag cgcgtcgtcg caccgggcaa
     3661 tgccgtaaat gctgcctgtc ggcatgacgg ccgcgggcac gtcgtagatc tggacttcct
     3721 ggccggaatg gctgttgtct tcgggccagc gaaaatgaat gcccccattc gtggtggcgg
     3781 ccgacgagcg gaaataacag cggaaatcga agtccgtgca gacggcttca tggaccaggt
     3841 ggccgttgcc gtcggcgccg cgcaggacgc cgtccgcagc cgcccaggtt gcctcaccgt
     3901 tcgccacggt ccatccggtg agggcctcat ccgtgggcag cacgattccg tgctccgagt
     3961 cgggcaaggg gatgatcgtc atgttgcgta cctggagggc gtcctggccg ctgttctgga
     4021 acccgatggc tccgcggcgg agggtatacc ccagtccggc cgcttccaga tctacgtcct
     4081 ggacgatctg gccattcatg gtcacgtgca aatggggcca gtccatgtgg atctcgcagg
     4141 tattccactc gccgggtttc ttacgcgcat tgactcgagg cgggatccgt ctgaagatgg
     4201 cgcccgtggt atagggcgtg tcgtgctctg tcgggtggcc ggacaactcc agttcgagcc
     4261 cggcggggta ctgccggtta cgcggcgcgt gcaggtacag accggtagcg gtccaggatg
     4321 cgacgttgaa ttcaaagcga agctcgaagt tttcatagtc ggccgcagtc agaagctcgc
     4381 cgggaaggcc ttccgctttt ttgaggctca gcgtacccgc atcgagaaca aacgagccct
     4441 cggaaccgcc ggactgggcc caacgctcca tcgagcccag gagctcgatc gggccatccg
     4501 cggcgactcc gggggcactc agggccaaaa caactggaac aagggcatac cagatcatga
     4561 gaggggacct ccttcccact tcttgggact gtttttggct gcaaaccacc atagtaccgg
     4621 tccaacggcc gagattccat atacttagcc tgaaaaccct catgcggtgg ggcggcctgg
     4681 gcataagccg gtggttctgg ccgcgtggac gtgaaatcag gcccggacgc cacaggcagg
     4741 gatggcatga acctgtaaca atacggcctc cgaccggtat catgctggtg agcctaaaga
     4801 tgatgtgaaa caggcggtta ggggctcacc agctgtcaat ttttaacctg tttggaactt
     4861 gacaagcccc tgaggggata gtagaatgtg gccgccgatg tgttccagca caggaacggg
     4921 cggatagtgt ccaaattcag ggaacagatt ggcagagaaa ggggactact cagatgaaac
     4981 acctcaagac attgaccagg cgaccggtcg aagcggcgga cattccgacg ttgatcaata
     5041 ttgtcgccct gattcaaggt ttgttgacgg cgatcctttc gtttactcag gcgaaagagg
     5101 gcgctgggac gtaaggcctg tattgcgcag tgcttacgta tgcggcggca cggcgcaacc
     5161 agtcggctct cagcgtgtac caaaaccgtc agtgctgttg agagggagca ttttaagtcc
     5221 gcacgggata tgcagcgaaa accgagggag agccaggatg aaacatattc gtacaatcac
     5281 ttcgaagcgg gcggatgcat tcagcgattt ctggaatgat ctctcgcggg catggtcgaa
     5341 tttcgtttac gccaagaaaa acgaagttgc cgtgtaggtt gacgaacgga ttctggaatg
     5401 ccggggcgct cagacaagtg aggtcccggc attcgttttt ttagcgggca tcctgtagac
     5461 tacgtgattt gagcgcggtg cggtggtgca ctgcgcccct ccgccgtatc gtaagaggga
     5521 aaaccggtga tttttggaga gcattgctgc attcgagcag cagagcccga cgacgccccc
     5581 gcgctgaaac gggtatatca gcgggcgaaa ccccgtgccg cggcgctgga tcgccgccgc
     5641 gagcccatta tccctaccac ggatgaactg cgggagctgc tgagcagcaa ggacgtcaag
     5701 ctgctgggcg aatattacgc ggtggaagac gccacgggcg agattcgagg attctgctgc
     5761 ttgcgcgccg gccgtcagga cgtattttac ggggaaatgc ttttgctgtt catcgaggac
     5821 gccgatctgg ataccggcat ggccggggag acctttgcgt tcctgaagaa caaggctttt
     5881 gtggaacgcc gcttgaacaa aatcgtcacc cagtgcctgg acaccgaaac ggctttgcgt
     5941 tcgtttctcc ttcggcaggg gtttaccagt gatgggatag cgcgcgaggt tctctatacc
     6001 ctcgggcgct ggtttaataa cgagacgctc tccttgcgtg cgtcgcagca cgaggatgga
     6061 tgatccatgg cccttcgcag caacattttt atccggcgag cagaacgtga agatctcgac
     6121 acggtggtcg aatggatgga agacccggat tttcagctgt ttctgtatgg cgacgtgacg
     6181 cgttcgcagc gccagatacg cgaacagatc gtgggcctgc tgggccgggc gccggggcat
     6241 accatgcccg cggcgttcca gctgatcctg gattcggagg cggatgggcc ggtgggtctg
     6301 gtcttgctgc agaacgtgag ctggcgaaac cgctcctgca gtctcgacgt gtatgtgggg
     6361 cagaagaatc tgcgcaaggg tatcgtgatc ggcctggcct tctaccgcat tatggaatac
     6421 tgttttgacg agttgaacct gcatcgcgtc agtgcgtata tctactcgtt taataccccc
     6481 tcgtggcgca tcatggaacg gagcaacgcc aagcgggagc tgacgctcaa ggaccacgtc
     6541 gtgcggaacg gcgagctgca cgacatgtac ggttatggcc tgttgcggcg ggaattcgat
     6601 gagttgcgtg agagtctcaa gggccgcatg gagggcaaat ctctcctgga gatgattgaa
     6661 gcgctgcacg ccaaagaaca gggggccggc gcgtgaggat tgctctgctg gccttcctcg
     6721 ggctgcatgt agcccatgca ctgtggctga atgggcgcca ggaccgtgtt tcgtcgaacg
     6781 cggagaaccg ggccagcctg tatctggttc aggtgccgct gttcgttttg ggctgcatca
     6841 ttgcctggca gaacggcgat gtggggcgag gcctggtgaa cccacttact atcgccgcag
     6901 gattgctgct ggggcatgtg atattcgtgc tctctctgct gctggtccac cgcagtgcgc
     6961 gcgacgcgcg ggaagttttg ctgggcctca ggacgatttg ggagttcgtc atcgagagcc
     7021 ccctgatcct gagccgctat gtgggggtgg cggtgacgga agaggtgatc taccgcgccg
     7081 cggcccagcg gattctgtgc acgtacttga gatcatcgtg ggcgggtatg gtgattgtgg
     7141 cggtgctgtt ttcggtggtc cat
//
LOCUS       DAOPBQ010000131         7162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9387_length_7162_cov_4.002673, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000131 DAOPBQ010000000
VERSION     DAOPBQ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9387_length_7162_cov_4.002673"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKW60_05780"
     CDS             complement(<1..702)
                     /locus_tag="PKW60_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98975.1"
                     /translation="MVTFEVNGTSYEGSAKKNLQVPGGMPIKNLKALFPDQFGLVGWS
                     APGSLIDGSLEEEIDSVSLFSETTAFPLVLTGWLTVPKSSEACSVTVIPPPGSKMGTS
                     EENSFYQYVTARPDELVMLRAVSKLKDSVVSAWTTKTNLQHTRSPQVVVPCSGEYNPV
                     LSTRHQYETLKLNFVDVLLEQTQGGRALASNSYWHGGRYASSLRVAWGEPTSFTACVS
                     PESEFVRWEFYRRDES"
     gene            complement(717..1700)
                     /locus_tag="PKW60_05785"
     CDS             complement(717..1700)
                     /locus_tag="PKW60_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98976.1"
                     /translation="MRCVLMLTTFLAATLAMPSALAGAESQAGTGNGDVPKEFRLVVN
                     ADQYAEIEQFLELVKHPGIYTLRPGLGGPQEGPEQSEQRQSRFSINSKSGWDYASLTY
                     DNRRQLVIGYHREATPEDSCDDCDIRVKTADDAYAIGKVMLPYYGLTPSIDEYTITLE
                     TFDPGVPGMKESKYWLIHKEFTLNGLPCRGSVFRCTVSSCRAIIDFVMYEEVVEPAAA
                     DLTVSRERARELAVGYLNRSLDIRYYAEKFVVRPSDEAREVIAKPRFRWHSFDEDGST
                     AKQVDFTKAYFCWEIPFDTWSNVTGLKSTNVLWIRRDTGKPIGGGIDRWSE"
     gene            2811..3395
                     /locus_tag="PKW60_05790"
     CDS             2811..3395
                     /locus_tag="PKW60_05790"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPJ98977.1"
                     /translation="MSDLDLVAQCRRGDTGAFDVLAGRYGQRLFHVAVRFLGNREDAL
                     DICQEAFVRAYQSLDRYRGDAQFYTWLYSIAANLARNRLRDRSRKGRDKGTSLDALLE
                     NAPAVAQAAAAAGRNPGTEAMEREMEEMLQRCLEELPEHYRLPFVLRTFDDLSYDEIA
                     EVMDCPAGTVKSRLNQARKLLRERLRDLAILEAE"
     gene            3410..4519
                     /locus_tag="PKW60_05795"
     CDS             3410..4519
                     /locus_tag="PKW60_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98978.1"
                     /translation="MSEKLSALLDGELSEGERQELEALIASNPAVAREWHALNRMDRL
                     FREMPRHDAPHDLTARLRAPRAVRRTVSFGRPRSSRRSAWPLLAAAAAFVFMFGLFLL
                     RIPKPDTITLTRLDSVPAESRSGHVSGQEEKACSAAAENRGPSPAAADDHWGEFGVVP
                     RQEIAAPMAAPAPAAGMRKREDAPQMQAGEAPAPTEAPEFLGGLGLEKGLAGGAASGA
                     RSPSPRGAADANEAHVEQKMGAVAGMRPQSSESDQAGALTRGAAPPPPPAAQAAPAIE
                     GVNAEITDGSETQHTGDRVFQLRDSIWVQDGYSEQAITAIERDSDEWAALIDADPTLA
                     WIMRLQEAVVFESAGAWYRIPPAMAAEQSDTPAQP"
     gene            5409..5756
                     /locus_tag="PKW60_05800"
     CDS             5409..5756
                     /locus_tag="PKW60_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98979.1"
                     /translation="MWFGKKDRPGGVPPHLRGGDQPAGPKPSFYRIEFDMLKKGAVFQ
                     RRGKPIRQFAVTVNMSTRVVTSGEVVDRETYEALVAAGAVDLPAETTFNADRGVAMHA
                     PAPPPGPPGSIEE"
     gene            5759..7159
                     /locus_tag="PKW60_05805"
     CDS             5759..7159
                     /locus_tag="PKW60_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ98980.1"
                     /translation="MGSSISLAMIVKDEGQVLEECLQSVRDLVEEIIIVDTGSHDETL
                     EIARGHGARIGGFLWCDDFGAARNESLRLCTKDWVLVLDADERIAPEDLPAIKRLVKG
                     PPAFAYEFTTRNYTNATNIANFQPCAANDPHARGFAGWHPSTKVRLFPAGAGVRFEGK
                     VHELVEPSLSRARLTIESCPVPIHHYPHLKEPNRTREKQEMYLRLGHDKVAQNPTDPK
                     GYIELGNQYAEVHDYEKAAGAYREALRLDPSNAVALRDLGGVLHLVNRSEEGKKALLL
                     AVRLDPRLAGAWRNLGVIYLDQKQWPAAVESFQNALKADPAWADGYRYLSIALESNGN
                     FSDAAEASRKALLSRPDCAECLRLYIHQMLRLERRKEARETLQRLIDKRASVAHVHNA
                     LGELYFYDDQLETAKSHFTRAGRLGSSAGYNNLGVVLMKERRYSEAAAAFEQCLQQDP
                     SHHGALSNLQKVRNRI"
BASE COUNT         1447 a         2180 c         2134 g         1401 t
ORIGIN      
        1 cgactcgtcc ctacgataaa attcccagcg aacaaactcg ctttccggcg aaacacaggc
       61 ggtaaaacta gttggttctc cccaagccac tcgcagcgaa gaggcataac ggccgccatg
      121 ccagtacgaa ttcgacgcca acgcgcggcc cccctgcgtt tgctccagca acacgtcgac
      181 aaaattgagt ttcagtgtct catactggtg gcgcgtgctc aggacagggt tgtattcacc
      241 cgagcacgga accaccacct gaggactcct tgtgtgctgg agattagtct tagtcgtcca
      301 cgcagacacc acagagtctt tcagcttaga aactgcccgc aacatcacaa gctcatcagg
      361 ccgcgccgtg acatactggt agaagctgtt ctcctcggac gtgcccattt tcgaaccggg
      421 aggaggaatc acggtaaccg aacacgcctc ggacgatttg gggaccgtaa gccatcccgt
      481 cagaacgagg ggaaaagccg tcgtttccga aaaaagcgag accgaatcga tctcctcttc
      541 caggctcccg tcgataagcg aaccgggggc tgaccagccc accaggccga actggtccgg
      601 aaaaagcgct ttgagattct taatgggcat accgccaggc acctgaaggt ttttcttggc
      661 actgccctcg tacgatgttc cattcacctc aaacgtcacc atgtggcctg ccgtccttat
      721 tcactccatc tgtcgatgcc gccaccgatg ggctttccag tatctctccg gatccaaagg
      781 acgtttgttg acttcaaacc ggtaacgttg ctccacgtgt caaacggaat ttcccaacag
      841 aaatacgcct tcgtgaaatc cacctgcttg gcggtgcttc catcctcatc aaaggagtgc
      901 cacctgaatc tcggtttcgc aataacttct cgtgcctcgt cactgggtct tacaacgaat
      961 ttttctgcat agtaacggat gtccagggac cgattgagat accccaccgc cagctctcgt
     1021 gctcgttctc ttgaaactgt caaatcagca gcggctggct caacaacctc ttcatacatg
     1081 acgaaatcaa tgatcgcacg gcaagaagaa accgtgcatc ggaatacact gccccgacaa
     1141 ggcagtccgt tcaaagtaaa ctctttgtga ataagccagt acttgctctc cttcattccg
     1201 gggacgccag gatcaaaggt ctcaagggtg atcgtatatt catcgatgga cggtgtcagc
     1261 ccgtagtagg gcagcataac cttccctata gcataggcgt cgtccgccgt cttgacgcgg
     1321 atatcgcaat cgtcgcaaga gtcttcaggc gtggcttcgc ggtgataacc gatcacaagc
     1381 tggcgtcggt tgtcgtaagt aagtgatgca taatcccagc cggacttgct attgatgcta
     1441 aaacggcttt gcctctgctc agactgctcg ggaccctcct gcggaccgcc gaggcccggg
     1501 cggagtgtgt aaatgcctgg atgcttcaca agttcaagga attgctctat ttccgcatat
     1561 tgatctgcgt tgacaacgag ccggaattcc tttggaacgt caccgttacc tgttcctgct
     1621 tgactctcag ctcccgccaa cgccgaaggc atcgcaagag ttgctgcaag gaaagtggtc
     1681 agcataagca cacagcgcat tgtaatgttc ctttctctac cattcagcat aaatctcggc
     1741 tgtctcaatc cgccgccccc gcatacctga ctcagcccat caatgcagca gaaacagcgg
     1801 cagcagaaac agcggaagca gaatcaacag gctggccttc tttcttctca tcacgcatca
     1861 ctcctgaact gtaccgcctc gcgagtgcac aggcgggggc aatattaacc cgagggcgaa
     1921 ccactgcgga tcgcccggcc cagtgcgcga gacttcgctg gattgaggcg cgcgggcgga
     1981 tctctggcga gaaagaaagt gttattcgcc tgggaggatg gcgtttcccg tgatatgccc
     2041 cgcagataag cccatcgttg cgcccctgtt gatgaaactt ctgccagttc tcgttttctt
     2101 atagctccca ttcggagtta tgtcaaggga ctgtcatatt tggtccatat tgtaagtgcg
     2161 ctagttttat ttttgaggaa aaggctgagg cttttgcgcc gggcgcccct ggctcgcgtg
     2221 ggcgtgagtt caacgctata tcgctccgac tgggctctgg aaaagggaat ccaaccccga
     2281 actccacccc caaatcggct atcgccgatt cacccccgcc agcgggggac agtgcgccgc
     2341 acagcaggct ctcaaattcg ggttcttgtg tctgctgcca gagacaatgg agcaaaaagc
     2401 agtccagcca ccgtgttcgc cggttccgtg ttttgtgtcc cccgctggcg ggggtggaca
     2461 cgcgtttcgc gtggccgggg gtggagaata ggcggccggc gagggccatg catccacgcc
     2521 ttcggtctat aggggcggac ccctgtggcc gcctagcccg ataactccac acgtccgtcc
     2581 atcctcacgc gtttatcttg atacagcgcc gtgcgccgcc agatcttctt tgtataccgc
     2641 gtgagcgggg cttcctggtc gagtatcgat ccggtgcatg ataaaatggc gtcaatcggc
     2701 aggggaaaac gaaccggcgg accggtttgg tgtcagaagg agagagcggc gaagcgctgg
     2761 ctccgcagct cgcaatttgc acggtggcag gttacagaaa cgccggcgcg atgtccgacc
     2821 ttgacctggt cgctcagtgc cgccggggcg atacgggcgc tttcgacgtg ttggccgggc
     2881 gctacgggca gcggctgttt catgtcgccg ttcgttttct gggcaatcgc gaggatgccc
     2941 tggacatctg ccaggaggcg ttcgttcgcg cgtatcagtc actcgaccgg taccggggcg
     3001 atgcgcagtt ttacacgtgg ttgtacagca ttgcggcgaa tctggcgcgc aaccggttac
     3061 gggaccgctc gcgcaaaggg cgcgacaagg gcacgtcgct cgacgctttg ctcgagaacg
     3121 cgcctgccgt cgcccaggcg gctgctgcgg caggccgaaa cccgggtacg gaggccatgg
     3181 aacgcgaaat ggaagagatg ttgcagcgct gcctggagga attgccggag cattaccgcc
     3241 ttccttttgt gttgcgcacc tttgatgatc tgagctacga cgagatcgct gaggtgatgg
     3301 attgccctgc gggcacggtg aaatcgcgcc tgaaccaggc gcgcaaactg ctgcgggaac
     3361 gccttcgcga cctcgccatt cttgaggctg aatgatggat acggagttct tgagcgaaaa
     3421 gttgtcggcc ctgctcgacg gcgaactctc cgaaggcgag cgccaggaac tggaggcgct
     3481 gatcgcgtcg aacccggcgg tcgcgcgcga atggcacgcg ctcaaccgga tggatcgcct
     3541 gtttcgcgag atgccgcggc atgacgcccc gcacgacttg accgcccggt tgcgcgcgcc
     3601 ccgcgcagtc cgccgcacgg tgtcgttcgg ccggccgcgt tcgtcacggc gctccgcctg
     3661 gcctttgctt gcagccgcag ccgcgtttgt gttcatgttc gggctgtttc tgctgcgaat
     3721 tccgaaaccg gacacgatta ccctgacccg cctggattcc gtgcctgccg aatccagaag
     3781 cggacacgtt tcagggcaag aagagaaggc ctgcagtgcg gcagccgaaa acagggggcc
     3841 ttcgccggca gcggcggacg atcactgggg cgaatttggg gtcgttcccc ggcaggagat
     3901 cgctgctccg atggcagcgc ccgcacctgc ggcaggcatg cgcaaacgtg aggatgcccc
     3961 gcagatgcag gccggggagg ctccagcgcc gaccgaggct cctgaattcc tcgggggcct
     4021 gggtctggaa aaaggccttg ctggaggcgc ggcctctggc gccagatcac cgtcgcccag
     4081 aggcgccgcc gacgcgaacg aggcgcatgt ggagcaaaaa atgggcgcag tagcagggat
     4141 gcggccgcaa tcgagcgaaa gtgaccaggc gggcgcactc acgcggggag ctgctccgcc
     4201 gccaccgccc gccgcccagg ccgcacctgc gattgaaggt gtcaacgccg agattaccga
     4261 tggcagcgaa acccagcaca ccggcgaccg agtctttcag ctgcgcgaca gcatctgggt
     4321 tcaggatggg tacagcgagc aggcgattac ggcgatcgag cgggattcgg acgaatgggc
     4381 cgccctgatt gacgcagatc caacccttgc gtggatcatg cggctccagg aagccgtggt
     4441 cttcgagagc gcaggcgcct ggtaccgaat ccctcccgca atggcggctg aacaatccga
     4501 cacgcctgct cagccctgag gcgcgcagcg cccgcgcgtt ttttctttcc gagtggaagg
     4561 aattgcggca gaactgaaga ctgcaagcca ttccagaaaa ctttccttgg aaccacttcg
     4621 aaatccaccc ccgaatcggc tgccgccgat tcacccccgc cagcggggga cagggcccag
     4681 cacgcgaatc tctgcggttc aggctcgtat agccgcgctg gcatggacgt cgacgtgtgc
     4741 cgcgtgggca cagcacgcgc agaggcacgt gctgcgcaag agaagaacgc aggcaagatg
     4801 cctgcgccac gcgctgcgaa gacttggatg taccagggaa atcccaacat tgccatcgag
     4861 ctctatacgt gcgccgattc ccacgtatcc atgcatccgg aatgcagggg cggccccctg
     4921 tggccgccca tccggatagc ccccgtggct gccatcctca cgcgctgact cggatgcaga
     4981 gcagccaccg ccggctccgc gtcttgtgtc ccccgctggc gggggtggcc acgcgcttcg
     5041 cgtggccggg ggtggactgc gtggacccgc gcatccgtct gaagaagggc aggcacgggg
     5101 gtctgcccct acgcgctgcg aaaacttgga tgtaccaagg aaatcccaac cttgccattc
     5161 atgtctatgc gtgtgccaag ccccacgtat ccatgcgtcc ggaatgttgg ggcggctcca
     5221 tggacgaggg tggacaatat cggtccacta tgcatgtttt tggacatatc gccgaatgcg
     5281 cagcgatacc gtcggcggca agatctcgga tgccgcgatc cgcgtcattg ttcgccgttt
     5341 ccgcgtcgtg gtacaatacg gctattattt gcggtgcgcc tttcatggtg cgacaggagt
     5401 caacgctggt gtggttcggg aaaaaagaca ggcctggcgg tgtgccgccg cacttgcgcg
     5461 gcggcgatca acctgccggg cccaagccgt cgttctaccg catcgagttc gacatgctga
     5521 agaagggcgc agttttccag cgtcggggaa agcccattcg gcagttcgcc gttacggtta
     5581 atatgtcgac tcgcgtagtg accagcgggg aagtcgtcga tcgggaaacc tatgaagcgc
     5641 ttgtcgccgc cggtgccgtc gacctcccgg ccgagacgac ttttaacgca gaccggggcg
     5701 ttgccatgca tgctcccgcg cctccaccag ggccccccgg cagcatcgag gaataggagt
     5761 gggaagttcg atctccctgg ccatgatcgt caaagatgag ggccaggtct tagaagaatg
     5821 tctgcagtcc gttcgggatc tggtcgagga gatcatcatt gtcgacacgg gttcgcacga
     5881 tgagaccctg gaaatcgccc ggggccatgg cgcccgcatc ggcggttttc tctggtgcga
     5941 tgatttcggg gcggcccgca acgaatcgct gcgtctgtgc accaaagact gggtcctggt
     6001 gctggatgct gacgagcgga ttgcgcccga agatctgccg gcgatcaagc ggctggtcaa
     6061 gggtccgccc gcatttgcct acgagttcac cacccgcaac tataccaatg ccaccaatat
     6121 cgccaatttt cagccctgtg cggctaacga tccgcatgcc cggggatttg ccggctggca
     6181 tcccagcacc aaggtccgcc tgtttcccgc gggtgcgggg gtgcgattcg agggcaaagt
     6241 tcacgaactg gttgagccgt cgctgagccg cgcgcggttg acgattgagt cgtgtcccgt
     6301 gcccattcac cattatccgc atctcaaaga gcccaaccgc acccgggaga agcaggagat
     6361 gtatctgcgc ctgggccacg acaaagtcgc ccagaacccc acggacccga agggttacat
     6421 cgaattaggg aatcaatacg ccgaagtcca tgactacgag aaggcggcgg gcgcgtaccg
     6481 ggaggcgctt cgcctcgatc cgtccaacgc ggtggccctc cgtgatctgg gcggggttct
     6541 tcatctcgtg aaccgctccg aggaaggtaa aaaggcgctc ctgcttgccg tccgcctgga
     6601 tccccggctc gccggcgcct ggcgcaacct gggcgtgatc tacctcgatc agaaacaatg
     6661 gcctgcagcg gtggaaagct ttcagaatgc cctcaaagcc gacccggcct gggccgatgg
     6721 ctaccgctat ctgagcatcg ctctggaaag caacggcaat ttttccgatg cggcagaggc
     6781 ttcccggaaa gccctgttga gccgcccgga ctgcgcggaa tgcctgcgtc tctatatcca
     6841 ccagatgctt cgcctggagc gccgaaaaga agcgcgcgaa accctgcagc gcctgatcga
     6901 caagcgtgcg tccgtcgcgc atgtccataa cgccctgggt gaactgtatt tttacgatga
     6961 tcagctggaa accgcgaaat cgcacttcac ccgcgccgga cgcctcggga gctccgcggg
     7021 ctacaacaat ctgggcgtgg tgctgatgaa agaacggcgt tacagcgagg cagcggcggc
     7081 gttcgagcag tgcctccagc aggacccctc ccaccatggc gcgctgtcca atctgcagaa
     7141 agtgcgaaac cgcatctagc cg
//
LOCUS       DAOPBQ010000132         7129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9443_length_7129_cov_3.982188, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000132 DAOPBQ010000000
VERSION     DAOPBQ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9443_length_7129_cov_3.982188"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2267)
                     /locus_tag="PKW60_05810"
     CDS             complement(<1..2267)
                     /locus_tag="PKW60_05810"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPJ98981.1"
                     /translation="MTAFLLTVFICASEVKHPVYPPDYTHEQTDALKKAVAPAMALSV
                     QELYDLVPEQSGIFFCGCPNCDGGAQEHALQWHLGMGDTVQCRYCGMQFPNEHFTSNR
                     STTLATPSGGIHEYRWYENEEGRQYYFEARAWYEQWQWTRDMALKLANLYALTGDFEY
                     GSRAAAIVGRYAQVFPDYAIRFDYPFRPVRFWPADQKWPYEPGFEPFRGAKFSWWGYG
                     DIPSRLALAYDLLASGDVFERMSDLLGTDIRTRIERDLIRLGYEFVVANPDDYTNMSP
                     GMYCDMIIAGRVLGAPEMVREALERAHTLVETQFFADGWWRECAPSYHWQTLGTLEAI
                     VRALRGYSDPALGPEPPAGEALLAEFIPMLARARQAGEEALLPDGRIMPLNDTWASDR
                     RAPLSESVSRLWPHMGHAILGAGTGETQFQVHINWNASYGHTHLDTGAILLFAHGKEM
                     LSDIGYTHTRYRNWVINSASHNMVVVDQRSQPLKYNQPAMGGNLLFFDDADPHVQLVD
                     LDASPAYPECSIYRRRLLHVHLREGFDYVVDCFDVAGGATHDFFLHGSADEPGTLQTA
                     IELHEPVASLVPDWGGTEPYTGEDCTDTSGERHHPYAFLRNIRAGACEGPLRLTWRYG
                     DTGLTTCLFPEPLSTACQFQSPAIRPAGEVDQHLDDHLMEGFLIRHSGGTSRFLAVHI
                     PWESTEWVTEVTQQAHELTITHETGRETIRLDENRITVTSSEGWEYATGTPAAGKIEA
                     IHTENGEAVIVCDCDV"
     gene            complement(2413..3405)
                     /locus_tag="PKW60_05815"
     CDS             complement(2413..3405)
                     /locus_tag="PKW60_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPJ98982.1"
                     /translation="MPMSSYEVVRRAIEFDRPDRLPLRFERLGLTDVRSVSWNQIGSG
                     NKSEKFSIDEWGCGWERSDVQNMGQVKEHPLQDWSALDSFSFPDPDTPAFYEGMERFF
                     EIDEDIYRCTDIFMLLFERMHALRGFENTLVDLYAEPERIGWLADRIIDFDLGIIRNM
                     YERFGDRIHGFGFTDDWGTERDLFISPELWVEFFKPRYKRIFDACHANGWHVWMHSCG
                     KVNDIIEHLIDIGLNVINLQQPRALGIEEIGERFRGRICFESLCDIQHTLPFKGAAEI
                     RAEAQLLLRHWGTPDGGFILSDYGDAQAIGVALDTKEIMLAAFLDADPWCAAKD"
     gene            3528..5444
                     /locus_tag="PKW60_05820"
     CDS             3528..5444
                     /locus_tag="PKW60_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPJ98983.1"
                     /translation="MRRRDFLCTAGGALAAAPLWARRSHAGETPPPNILWLTCEDISP
                     QLGCFGQPGATTLNLDRLASEGARYVNAFSVSGVSAPSRSCLITGMYPNTLGTQHMRC
                     KTPLPPQIRCFSEYLREAGYYCTNNAKTDYQFDPPAQAWDESSKDAHWRNRPDPAQPF
                     FAVFNQTITHESEIGTLFENEEVLRKRLEPQAPHALDALDLPPYFPDTPVIRRYWAHY
                     FDLITIMDHWVGAMLNQLEEDALAENTLVFFFSDNGAGLPWCKRWLYDRGIRVPLIVR
                     WPGKLPPGAERDELVSFVDFAPTILSCAGLPIPAYMQGRAFLGSATSPTRNYVFAARD
                     RMDERYDIIRAIRDKRFKYIRNYEPYRPYDQHLVYPERFPVMQELRRVEKEGALEGAQ
                     KLFFAGTKPLEELYDMHADPNEIENIVDQPEFAHELGRLREALDVFLDDSADTGFIPE
                     VDLSAWLAGRWAGPPETLPEYELASSETIFGKPVKTYVDLLNGSNRLRRLRAIKTLGL
                     VPEALPLLRHALEDYDPAVVYWAAVALGHLQDDSPEIREKLLKALGRKELSARLGAAR
                     GLVLCGYINDAMPGLLQVLADEDEYARLSAVETLEIAGLEHPGVRDALEKVMQDKETY
                     PRRIAATALGKSIR"
     gene            5712..6002
                     /locus_tag="PKW60_05825"
     CDS             5712..6002
                     /locus_tag="PKW60_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPJ98984.1"
                     /translation="MNIYVGNLSYDTDNASLRALFETHGAVDDARVIEDRQTGRSRGF
                     GFVEMPNSSEAQAAIDALNGHEIQGRTLTVNQAREREARRPRGGGGGYGSRY"
     gene            complement(6068..>7129)
                     /locus_tag="PKW60_05830"
     CDS             complement(6068..>7129)
                     /locus_tag="PKW60_05830"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPJ98985.1"
                     /translation="AAHFDERLHMWRETTKHERNRRIWEEELEDFVPARVLDFHVHLF
                     DEATIPSGCEAYSCAGHPIRSYDLAALSQDLDELYPKRETSAVVFGLPHVEYDLQANN
                     AYVSEVCDGKRFFPLRLFDPETDTPEQLEADLASGRFLGLKPYLNYVRKPDLNEVQIS
                     EMLPNWAMEIVNARGLLVMLHIPRKDRLADPLNQIQLRDLCRSYPAARIVLAHVGRAY
                     FLKNVRGTLDNLTDLPNLYYDLAMVSNPDVLEYVFRTAPRERVLYATDLPIAVAPGKS
                     VEINNQYTYITPVPWPLSLSDDRGKLVFTSFLYEELRGIRTAVERCELGSDFVDALFY
                     DNGQRLLDAVQKPGGVRTI"
BASE COUNT         1439 a         2130 c         2107 g         1453 t
ORIGIN      
        1 acgtcgcagt cgcagacgat cacggcctcg ccattttcag tgtggatggc ttcgatctta
       61 cccgcggccg gcgttcccgt tgcatattcc cacccctcag atgaggtcac cgtaatacgg
      121 ttctcatcca gccgaattgt ctcgcggccg gtttcgtgcg taattgtcaa ttcgtgtgcc
      181 tgttgcgtga cttctgtaac ccactctgtg ctttcccagg ggatgtgaac cgccaggaaa
      241 cggctggtcc ctccagaatg acggatcaga aacccctcca taagatgatc gtccagatgc
      301 tggtcaactt cgccggcagg gcggatggcg ggggactgaa actggcaggc cgtggagagg
      361 ggttccggga agaggcaggt ggtcaggcca gtgtcgccat aacgccacgt cagacgcagc
      421 gggccttcgc acgcgcccgc ccgaatgtta cgcaggaagg cgtagggatg gtgccgttct
      481 ccactggtgt cggtgcaatc ctcccccgtg tacggctcgg ttccgcccca atccgggacc
      541 agcgaggcca ccggctcatg cagttcgatg gccgtctgaa gcgtcccggg ttcgtctgcg
      601 cttccgtgca gaaaaaagtc gtgtgtggcg ccccctgcaa cgtcgaagca atccacgacg
      661 taatcaaatc cctcccgaag atgcacgtgc agcaggcgtc tgcggtagat agagcattcc
      721 ggatatgccg gactggcatc aaggtcgacc agctggacat gaggatctgc atcgtcaaaa
      781 aacagcagat ttcctcccat ggcgggctgg ttatacttca aaggctgcga tcgctgatca
      841 acgactacca tgttgtgaga tgccgagttg ataacccagt ttcggtaccg ggtatgcgtg
      901 tagccgatgt cggagagcat ttccttgccg tgcgcgaaca gcaggatcgc gcccgtgtcc
      961 aggtgggtat gtccatacga cgcgttccag ttgatgtgca cttgaaactg ggtttcgccg
     1021 gtccccgcgc ccagaatcgc gtgtcccata tgcggccaga gccgcgagac cgactcgctg
     1081 agcggcgccc gccggtccga agcccacgtg tcgttgagcg gcataatgcg cccatccggg
     1141 agcaaggcct cttcgccggc ctggcgcgcc cgggccagca tggggatgaa ttccgccagg
     1201 agcgcctctc cggcgggcgg ttccggaccc agggcagggt cgctgtaacc gcgcaacgcg
     1261 cgcacaatgg cctcgagcgt cccgagtgtc tgccagtgat aacttggggc gcactcccgc
     1321 caccagccgt ccgcgaagaa ttgcgtttcg accagggtgt gtgcacgttc cagcgcctcg
     1381 cggaccatct ccggcgcgcc aaggacccgc ccggcaatga tcatgtcgca atacatgccc
     1441 ggcgacatgt tggtgtagtc gtccgggttg gcaaccacga attcatagcc caggcggatc
     1501 agatcccgtt cgatgcgcgt ccgaatgtcc gtgcccagca agtcgctcat gcgctcgaac
     1561 acatctccgg atgccagcag atcatacgcc agcgcgagcc tgctggggat gtccccgtaa
     1621 ccccaccagg agaattttgc tccgcgaaat ggctcgaaac ccggttcgta cggccatttc
     1681 tggtcagcgg gccagaaacg caccggccga aacgggtaat caaagcgaat tgcatagtcg
     1741 ggaaacacct gtgcgtagcg gcccacgatg gcggcggcgc ggctcccgta ttcgaagtcg
     1801 cccgtgaggg catacaggtt ggcgagcttc agcgccatat cacgggtcca ctgccactgc
     1861 tcgtaccagg cgcgggcttc aaaataatac tggcggcctt cctcgttttc gtaccaccgg
     1921 tattcgtgta tcccgccgct cggcgttgcg agggttgttg aacggttcga tgtgaagtgc
     1981 tcgttgggaa actgcatgcc gcagtaccgg cattggaccg tgtcgcccat gcccagatgc
     2041 cattgcagag cgtgctcctg cgcgccaccg tcgcaattcg ggcagccaca aaagaaaatg
     2101 cccgactgct ccgggaccag gtcatataat tcctgcaccg acagcgccat cgcgggggca
     2161 acggcttttt tcagcgcatc tgtttgttcg tgggtgtagt ccggtggata gaccgggtgc
     2221 ttcacctcag atgcgcagat aaaaacagtc aagagaaagg ctgtcatcgg aaagagtcct
     2281 ctgttcctga gagctgggtc cgtatggtaa aggtccgtaa gggaattgtg caaaacgggt
     2341 cgaagcctct acatttaccc tcttcgaact ccgtagttca tagcaaaaac gcgcggggga
     2401 gaggctttcc gttcaatcct tcgccgcgca ccatgggtcg gcgtccagga atgccgcgag
     2461 catgatctcc ttggtgtcaa gcgcgacccc gatagcctgt gcatcgccat aatcagacag
     2521 gatgaacccg ccgtccgggg tgccccaatg ccgcagtaga agctgcgctt ccgcgcggat
     2581 ttcagccgcg cctttgaatg gcagcgtatg ctggatgtcg cacaaggatt cgaagcagat
     2641 gcggccgcgg aaacgctcgc cgatttcttc gatccccagg gcgcgcggct gctgcaggtt
     2701 gatgacattc aacccgatgt cgatgaggtg ctcgatgatg tcgttcactt taccgcagga
     2761 atgcatccag acgtgccatc cattggcatg gcaagcatcg aagatgcgct tgtaccgggg
     2821 tttgaaaaat tcgacccaca gctccgggct gatgaacagg tcgcgttcgg taccccagtc
     2881 atctgtgaat ccgaagccgt ggatcctgtc gccgaagcgc tcgtacatgt tccggataat
     2941 gcccaggtca aagtcgatga tacggtcggc gagccagcca atgcgttccg gctcggcgta
     3001 gagatctacg agcgtattct caaagccgcg cagcgcgtgc atccgttcga ataacagcat
     3061 gaaaatgtca gtgcagcggt aaatgtcctc atcgatctca aaaaatcgtt ccatgccctc
     3121 ataaaatgcg ggcgtatccg ggtcggggaa ggaaaacgag tcaagcgcgg accagtcctg
     3181 cagtggatgc tccttgactt gccccatgtt ctgcacgtcc gagcgttccc atccgcagcc
     3241 ccattcgtcg atcgagaatt tttcgctctt gttgccggac ccgatttgat tccaggagac
     3301 cgaacgcacg tcggtgaggc cgagcctctc aaatcgaagc ggcaggcgat caggccggtc
     3361 aaattcaatg gcgcggcgga caacttcata cgatgacatg ggcatggcat atccctcggc
     3421 ttgggcggcc tttacagtta tcaggcgcgc catgatacca tgtgtctggc ttggcgctgc
     3481 gctccgccaa gcgatcatca tggtaaggat caggtgggga aggagtcatg cgccgtcgtg
     3541 attttttgtg cactgccggt ggcgcgctgg ccgctgcacc attatgggca cggcgcagcc
     3601 atgccggcga aacccctccg ccgaatattc tgtggctgac ctgcgaggac atcagcccgc
     3661 agctcggctg cttcggtcag cccggcgcca ccacgctcaa tctcgatcgc ctggcctcgg
     3721 aaggcgcccg gtacgtcaac gccttttcgg tgagcggcgt ctctgcgccc agccgctcct
     3781 gcctgatcac gggcatgtat cccaacacat tgggcaccca gcatatgcgc tgcaaaacgc
     3841 cgcttccgcc gcaaatccgg tgcttttcgg aatatttgcg ggaggccggc tattactgca
     3901 ccaataatgc gaagaccgac taccagtttg acccgcccgc gcaggcctgg gacgaatcca
     3961 gcaaggacgc ccactggcgc aaccgccccg acccggccca gcctttcttt gcggttttca
     4021 atcaaaccat cacgcatgag tctgaaatcg gaacgctttt cgagaacgag gaggtcctgc
     4081 gaaaacgtct ggaaccgcag gccccgcatg cgctcgacgc gctcgatttg ccgccgtact
     4141 tccccgacac acctgtcatt cggcggtatt gggcccacta tttcgacctg atcacgatta
     4201 tggaccactg ggtgggcgcc atgttgaacc aacttgaaga agacgccctc gcggaaaaca
     4261 cgctggtctt ctttttcagc gataatggcg ccggactgcc ctggtgcaaa cggtggctgt
     4321 acgacagagg gattcgcgtg cctttgatcg ttcgatggcc gggcaagctt ccgccgggcg
     4381 ccgaacggga cgaattggta agcttcgtgg attttgcccc caccatcctg tcgtgcgcgg
     4441 gccttcctat ccccgcatac atgcagggca gagcctttct cggcagcgcg acaagtccca
     4501 cccgcaacta cgtctttgcg gcgcgcgacc gcatggatga acgctacgac atcatccggg
     4561 ccatccgcga caaacgcttc aaatatatcc ggaattacga gccctaccgc ccctacgacc
     4621 agcatctggt ttatccggag cgcttcccgg tcatgcagga gttgcggcgc gtcgaaaagg
     4681 agggtgcgct ggaaggagca cagaaactct ttttcgcggg gaccaaaccg ctagaagaac
     4741 tatacgatat gcacgcggat ccaaatgaaa tagaaaatat cgtcgaccag ccggagttcg
     4801 cgcatgaact cggccgcctg cgtgaagcgc ttgacgtgtt tcttgacgac tccgccgaca
     4861 cggggttcat cccggaggtc gacctgagcg cctggcttgc tggaaggtgg gcaggccctc
     4921 cggagaccct tcccgagtat gagcttgcca gcagcgagac gatcttcggg aaaccagtaa
     4981 agacttacgt ggatctgctg aacggctcga accggctacg ccggcttcga gctatcaaaa
     5041 cccttggcct cgtacccgag gcattaccac tgctgcggca cgccctggaa gattatgacc
     5101 cggcggtggt ttattgggca gctgtcgcgc tgggccacct ccaggatgac tcgcccgaga
     5161 tccgcgagaa gttgctcaaa gcattgggaa gaaaagagtt gagcgccaga ttgggcgcgg
     5221 ctcgaggcct ggttttgtgt ggatacatca atgatgccat gcccggctta ttgcaggtgc
     5281 tggcggacga ggacgaatat gcacgcctgt ctgcggtcga aaccctggag atcgccggcc
     5341 ttgaacaccc gggcgttcgt gacgccctcg aaaaggtcat gcaagacaaa gagacctacc
     5401 ctcgccggat tgctgcgact gccctgggga agtcgatacg ttgacgcacc cggcagaagt
     5461 ctcaggccgt ctcgctgagc tgccggggcc gccgttttcc gtctgtgtgc aatgtattgc
     5521 ttttttgggg gctctcggct acactactac tcgtgcaatg gtgcacatga actggagtta
     5581 gtgagcttct gcctccgttt tggacctgat cctgcgcgtg atttccgaaa agatgtcatg
     5641 ctcgctccgg actggttgcc gaccgggttg agggccaccc tccacaagac cgctctggaa
     5701 aggaaagagt aatgaacatc tacgtgggaa atctgtcgta tgacacggac aacgcatcgc
     5761 tgcgcgcgct ttttgagacg catggcgcag tggacgacgc gcgggtcatc gaagaccggc
     5821 agacgggccg ctcgcgcggg ttcggtttcg ttgagatgcc gaacagctct gaagcgcaag
     5881 ccgctatcga cgccctcaac gggcatgaaa ttcagggccg caccctgacc gtcaatcagg
     5941 cacgtgagcg cgaagctcgc cgtccgcgtg gcggcggcgg tggttacggc tcccgctact
     6001 agtccttacg gcaacaagcg aaggcggtac ttcctcgtta cgcggggttg taccgccttt
     6061 ctttgtgtca gattgtgcga actcccccgg gcttctgaac ggcgtccagc agcctctggc
     6121 cgttatcgta aaacagagcg tccacgaagt cgcttcccag ctcgcagcgc tccaccgcgg
     6181 tacgaatgcc ccgcagttct tcgtagagaa acgaagtgaa caccagcttt ccgcggtcat
     6241 ccgacagcga cagcggccac ggtacagggg tgatgtatgt gtattggttg ttgatctcga
     6301 cagatttgcc cggcgcgacc gcaatcggca gatccgttgc gtacaggact cgttcccggg
     6361 gcgctgtccg aaacacgtat tccagcacat ccggattact caccatggcc aggtcgtaat
     6421 agagattggg caggtccgtc aggttgtcga gggttccccg cacgtttttg aggaaatacg
     6481 cgcggcccac gtgcgccagt acgatgcgcg ccgccggata ggaccggcag agatcccgga
     6541 gctgtatctg attgagcgga tcggccaggc gatctttgcg gggaatgtgc agcatgacga
     6601 gcaggccccg cgcgttcaca atttccatgg cccagttcgg gagcatctct gaaatctgta
     6661 cctcgttgag gtccggtttc cgcacgtagt tcagatacgg tttaagcccg agaaaacggc
     6721 cggatgcgag gtccgcctcc agctgctccg gtgtatccgt ttcggggtcg aacaggcgca
     6781 gcggaaagaa gcgctttccg tcgcatacct cgctcacgta ggcgttgttg gcctggaggt
     6841 cgtattcgac gtggggaaga ccgaagacca ccgcactggt ctcgcgtttc ggatacagct
     6901 cgtccagatc ctgggaaagt gcagcaagat catacgagcg aatcgggtgt cctgcacacg
     6961 aatacgcttc gcatcccgat gggatagtgg cctcgtcgaa gaggtgcaca tgaaaatcga
     7021 gaactcgcgc cggtacgaaa tcttcgagct cctcttccca aatacgacgg tttcgctcgt
     7081 gtttggtcgt ttcccgccac atatgcagcc tttcgtcaaa atgcgcggc
//
LOCUS       DAOPBQ010000133         7115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9470_length_7115_cov_3.768555, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000133 DAOPBQ010000000
VERSION     DAOPBQ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9470_length_7115_cov_3.768555"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKW60_05835"
     CDS             <1..275
                     /locus_tag="PKW60_05835"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPJ98986.1"
                     /translation="MHVAPHARLDSRAFDVYVIGDVGKAEAVVNLPKLYRGRLLRKPD
                     KVRYFRASRVLADSSQEVLLNLDGEQPGRLPVTIQLLPSVLKVVVG"
     gene            420..1688
                     /locus_tag="PKW60_05840"
     CDS             420..1688
                     /locus_tag="PKW60_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98987.1"
                     /translation="MRMLCITLALSGLAATTACAAFQAGVAVRDVTPDPLLPVSGGAG
                     PSSPASQQTGNLTVRALVFDRDGQRVAIVGADFLGFPGALCDRVRAKVPGIPAENILI
                     GSTHTHSAPDMYGFPDGQGGHGANLEYIALVVDRMADAINAAVASLAPASLKINTGEA
                     QGKIAYNYYAPKLYDPRCHVIQAIGQDGKAIATLINYAIHPEVIGPDQGILSPDLCGP
                     LYDRVAEKGGGTAVFMNSAQGGMVTADCRGPDDKDIQTWDECVRIGTLLADEALRIVS
                     EAPLQSDPSLYCTARNVTFDVDSEEIRMLATASPVGMMLNPDNSVTTRINLVNIGNAQ
                     ILTIPGEALPNIGYYLKRHMNGDHHLLFGLTNDAYGYIMAKVDWNSFKRYDYISSLNL
                     GEFTGEHLMEECLALVKASPKPDKLAKAGN"
     gene            complement(1747..3183)
                     /locus_tag="PKW60_05845"
     CDS             complement(1747..3183)
                     /locus_tag="PKW60_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HPJ98988.1"
                     /translation="MNMLTESSARERLEAHGQEQVLRFWGQLNDSERPHLLAQVESID
                     FKLLNRLVAQWVTSEPPEERFGCIEPVPVIPKGGGQDAREAWQAGEEALKEGRVGLLL
                     VAGGQGTRLGFDGPKGAYPIGPITGKSLFGYHAEKIRNLQRRYQCILPWYIMVSNTNH
                     AQTRAFFEKNHYFGLQPENVKFFEQRMVPCVDQGGKLMLDQPGCLAMNPNGHGGVIPA
                     VVDNGIARDARNRGIDTLSYFQVDNWAVKVADPLFIGCHVMRSAMMSSKTHRKEQPRE
                     AVGVHCLCDGVYRVIEYTVLDLYPQLLETDSSGHLLHYAGNPAIHILNVGFIESLNAN
                     YDNFPWWRAHKKIPCLNEQGERIEPRKPNGYKFETFIFDALRFVTHDPVVLEIPRLGE
                     YTPIKSFEGDNSALAARETMSALWGGWLERAGFPVRRDAADKVAVDIEISPDFASSEQ
                     EFIERARERQYDALSDVAIGPDGRLENR"
     gene            complement(3255..4385)
                     /locus_tag="PKW60_05850"
     CDS             complement(3255..4385)
                     /locus_tag="PKW60_05850"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPJ98989.1"
                     /translation="MRIRPAAIVIIVAAAIGAFVLYAALSSRETPGTQFSIPSGESSG
                     GAMLPPVDENQDLVPILDIGMDRFDAGLVPNDKATVKTIPVKNTGRKKLVIRQITTTC
                     ACTTGKITPMEIMPGGTATMEITIEPDRVAGFYSAKQLTILSNAPATPMKELLVVASI
                     DPEFEVDPEMIEFGEVEKGARVQRQVVLRQIGDEPIELLGLESMDKKDKTLSPSFVKL
                     PESEWRTPGKPEYLITVALEPFAPLGKHTKSLVIETTCKRVPRLRLLCEADITGFYEM
                     EQQEMAFTWNRAKIHEEPAGTLIVAADRPIEIVDARVSDEMFVVKTVPGPRPNTMAIE
                     LYIQGVPEVGRVDDELTFAVKAEGTGLLPNKVPIRGTIVDAK"
     gene            complement(4389..5399)
                     /locus_tag="PKW60_05855"
     CDS             complement(4389..5399)
                     /locus_tag="PKW60_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPJ98990.1"
                     /translation="MKYYMGLDVGATKTFCLIADETAAIRGFGRAGNGSYEVQGIAAA
                     ARENQQAVRNALDAAGLTLADIAWVGMGVAGADLPEDFVMLEREIYTPLFGSTPRVFR
                     NDSMAAMRGSVRTSYGIVIACGTGVIAAGINPQGQEARAGGLNEEFGDLWTGTIIGRQ
                     GLYAVYHARDGVRPPTLLTDLFVEKAGCSDVDEFFYKMYRREMSMDALQPMARLVFDA
                     AFQGDSAALEILRAAGGYLGELVIAVARKLEMSRQPFDLVTAGSVFKGSSPVLKDCMM
                     ARVHEVCPLAKAVTPLFEPVVGALFMALEKDVDMTDSIYENLADSLLEAGKQSNVEFT
                     AK"
     gene            complement(5624..6538)
                     /gene="ybgF"
                     /locus_tag="PKW60_05860"
     CDS             complement(5624..6538)
                     /gene="ybgF"
                     /locus_tag="PKW60_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HPJ98991.1"
                     /translation="MRVSFMIAAPLLGMLLLAGCATTGGPNDPVANTIYDMHRKVSRL
                     EKELGGTVEHLNVTATDLGVRVNETNTQSRELQGLVEENQQKLAQLEKKLDSLTVNVY
                     KALGITVPSSSQSSTGWGAGGQSDVVPGRVQVEPPSAAVAAPPAYGLATEPDPLADMG
                     AAVQPAAATAAAPAPAGALDPVPAYNKAMEAYQRDQYDEALNLFSDFMARFPKSEIRD
                     NAQFWKGECLYNLGRYDECIAEFEALRAQHPESAKVPYGMFNQAAAHLKLGQQERAFA
                     LLEDLVENYPMTPAAARARSKLEEVKGN"
     gene            complement(6551..7087)
                     /gene="pal"
                     /locus_tag="PKW60_05865"
     CDS             complement(6551..7087)
                     /gene="pal"
                     /locus_tag="PKW60_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HPJ98992.1"
                     /translation="MSYKTSMKLFVLALVIVAVAVGGCGRKKTQITPDLTGDTTSRQS
                     AGPESTSGEGLPSLEQEKLFFQQLAEAGTVYFAYDSSQLDPTAMGTLKRNAEIIKQMP
                     NSVIQIEGHCDERGTQQYNLALGERRALAVRDYLIRLGISGDRLVTISYGEEDPVDPG
                     HTESAWAKNRRAEFNRAN"
BASE COUNT         1386 a         2131 c         2115 g         1483 t
ORIGIN      
        1 ggatgcacgt cgcgccacat gcccgtctcg acagccgcgc tttcgatgtc tacgtaatcg
       61 gcgatgtggg caaggcggag gccgtcgtta accttcctaa actctaccgg ggccgactct
      121 tacgcaagcc cgacaaggtt cgctattttc gcgcatcccg cgtgcttgcg gattcgtcgc
      181 aggaggtgtt gctgaacctc gatggggaac agcctggccg tttgcctgtg accattcaat
      241 tgctgccgtc ggtgctgaaa gtagtggtcg ggtgagagga ctggaaggcc tccccagggg
      301 gaatcgcgcc gcggatctct ggcgttttat tttctcagag cataaggtgg tgtattctgt
      361 gggaagacgc ttgttcagcg aattgcggtg gaaacaaacg aggagattct gggatgaaca
      421 tgcgaatgct gtgtatcacg ctggccctgt cgggattggc cgcaaccacg gcctgcgcgg
      481 catttcaggc gggagtggcg gtacgcgacg tgactccgga ccctctgctg ccggtgagcg
      541 gcggcgcggg tccgagcagc ccggcctcgc aacagaccgg caacctgacc gtgcgtgccc
      601 tggtgtttga ccgggacgga caacgggtgg ccattgtagg ggcggatttt ctgggatttc
      661 cgggggcctt atgcgatcgc gttcgggcga aggttccggg catcccggcg gagaacatcc
      721 tgatcgggtc aacccacacc cacagtgcgc ccgacatgta tgggttcccc gacggccagg
      781 gtgggcacgg agcgaacctg gagtacattg ccctggtggt cgatcggatg gccgacgcga
      841 tcaacgcggc cgtggcgtcg cttgcaccgg cctcgttgaa gatcaacaca ggcgaggccc
      901 agggcaaaat cgcatacaac tattatgcgc caaagctcta tgacccgcgc tgccatgtga
      961 tccaggccat tggacaggat ggaaaagcca ttgccacgct gatcaactat gcgatccacc
     1021 ccgaagtgat cggaccggat cagggcattt tgagtcctga cctgtgcggc ccgctgtatg
     1081 accgcgtggc cgagaaaggc ggtggcacgg ccgtgttcat gaacagtgcc cagggcggga
     1141 tggtcaccgc ggactgccgg gggcccgacg acaaggacat tcagacctgg gacgaatgcg
     1201 tgcgcatcgg gaccctgctg gcggatgagg cgctgcggat tgtgtccgag gcccctctgc
     1261 aatcggatcc ttcgctatac tgcacggccc gtaacgtcac cttcgatgtg gattcggaag
     1321 agattcgcat gctcgccacc gcgtcgccag tcggcatgat gctgaacccc gacaacagcg
     1381 tgactacacg gataaacctc gtcaatatcg gcaacgcgca gattctgacc atcccgggcg
     1441 aggccctgcc caacatcggg tactacctga aacgccacat gaatggcgat caccacttgt
     1501 tgttcgggct gaccaatgat gcctacggtt acattatggc caaggtggac tggaacagtt
     1561 tcaaacgcta cgactacatt tccagcttga atctcggcga gttcacgggg gagcatctta
     1621 tggaagagtg cctggcgctc gtaaaagcga gcccgaaacc cgataagctt gccaaagccg
     1681 gtaattaaac acgagccgcc tccacgcggc cggggctgac atcgaggcac gcagccgcca
     1741 accggctcag cggttctcga gacgtccgtc ggggcctatc gcgacatcgg acaacgcgtc
     1801 gtactgccgc tccctggcgc gttcgatgaa ttcttgttcg ctgctcgcaa aatcagggct
     1861 gatctcgatg tcgacagcga ccttgtccgc ggcatcccgg cggacaggaa acccggcgcg
     1921 ctcaagccat ccgccccaca gtgcggacat ggtttcgcgc gcggcaagag cgctgttatc
     1981 tccctcaaag gatttgatgg gggtgtattc tccgaggcgt ggaatctcca gaacgacagg
     2041 gtcgtgggtg acaaaacgca gcgcgtcaaa gatgaaggtc tcaaacttgt atccattggg
     2101 tttgcggggt tcgatgcgct caccctgttc gttgaggcag gggatctttt tgtgcgcgcg
     2161 ccaccagggg aagttatcgt aattggcgtt cagggattcg ataaacccaa cattcaggat
     2221 atggatggcg gggttgcccg cataatgaag caggtggccc gaggaatcgg tctccagcaa
     2281 ctggggatag agatcaagga ccgtatattc aatgacgcgg taaaccccgt cacaaaggca
     2341 gtgcactccg accgcttcgc gaggctgttc cttgcggtgg gtttttgacg acatcatcgc
     2401 ggaacgcatg acatggcagc cgatgaaaag cggatcggcg accttgaccg cccagttgtc
     2461 gacctggaag tagctcaggg tatcgattcc gcggttccgg gcgtcccggg cgattccgtt
     2521 atccacaaca gccggaatga cgccgccgtg gccattcggg ttcatggcaa ggcagccggg
     2581 ctggtccagc atgagtttgc cgccctggtc gacgcagggg accatgcgtt gctcgaaaaa
     2641 tttcacattt tcgggttgaa ggccgaaata atgatttttt tcaaaaaaag cccgggtctg
     2701 cgcatggttg gtgttgctga ccatgatgta ccagggcagg atgcattggt aacggcgctg
     2761 aagattgcgg atcttctccg cgtggtaacc gaagaggctc ttcccggtaa tggggccgat
     2821 cgggtatgcg cctttgggcc catcgaaccc gaggcgcgtg ccctggccac ccgcgacaag
     2881 caggagcccg acccggccct ctttgagcgc ctcctcgccg gcctgccatg cctcccgggc
     2941 atcctgcccc ccgcctttcg gtatcacggg cacgggctcg atacagccaa atcgttcttc
     3001 gggcggctca gacgtgaccc attgcgcgac cagtcggttc aggagtttga aatcgataga
     3061 ttcgacctgc gcgagcaggt gcggacgctc cgaatcattg agctggcccc agaaacgaag
     3121 cacctgctcc tggccgtgtg cttccagacg ttcgcgcgca ctggattccg tcaacatgtt
     3181 catttactct ccacaaaact ggatattctt cagccggtgg tacagtgcca ggccatccta
     3241 ttcgcagccg gtattcattt ggcatcgaca atggtgccgc gtatcggaac cttgttcgga
     3301 agcagcccgg tgccctccgc cttgaccgcg aacgtcagct catcatccac ccgccctacc
     3361 tccgggactc cttgaatgta caactcgatg gccatcgtgt tggggcgggg tcccgggacg
     3421 gtcttcacga caaacatctc gtccgagacc cgggcatcga cgatttcaat cgggcggtcg
     3481 gccgcgacga tcagggttcc ggcgggttct tcgtgaatct tcgcccggtt ccaggtgaag
     3541 gccatctcct gctgctccat ctcgtaaaag cctgtgatgt cagcctcgca cagaagacgt
     3601 aagcggggca cacgcttgca ggtcgtctcg atgacgagcg attttgtgtg cttgcccagg
     3661 ggtgcaaaag gctcgagagc aacggtaatg aggtactccg gcttgccggg ggtccgccat
     3721 tcgctttccg gcagttttac gaacgaaggc gacagcgttt tgtccttttt gtccatggat
     3781 tcaagaccga gcagttcgat gggctcatca cctatctggc ggagcacaac ctgccgctga
     3841 acccgggctc ctttttcgac ttcgccaaat tcgatcattt cgggatcgac ctcgaattca
     3901 ggatcgatac tggcaaccac caggagttct ttcataggcg tcgcgggcgc attcgacaga
     3961 atcgtcagct gctttgctga ataaaagccg gcaacccggt cgggctcgat cgtgatctcc
     4021 atcgtggccg ttcccccggg catgatctcc atgggagtaa tcttcccggt ggtgcaggcg
     4081 caggtggtcg tgatctggcg aatgacgagc tttttgcggc ccgtattctt cacggggatc
     4141 gtctttacgg tcgccttgtc attgggcacg aggcccgcat caaagcggtc catgccgata
     4201 tcaaggatcg gcaccagatc ctgattctca tctaccggag gcagcatcgc ccctccggaa
     4261 gattcgccag acggtatgct gaactgtgtt ccgggagtct cccggctgct cagagcagca
     4321 tacagcacaa aggcgcctat cgcggcggca acgatgataa caatggcagc aggtcgtatg
     4381 cgcatggctt attttgcagt aaactccaca ttggattgtt ttccggcttc caacaggcta
     4441 tccgcaagat tttcgtaaat ggaatccgtc atgtcaacgt ctttttcgag cgccatgaac
     4501 agcgcgccga ccacgggctc gaacaggggg gtcacggcct tcgccagagg gcagacttca
     4561 tgtacgcgcg ccatcatgca atctttgaga accggactgc tccccttaaa tacactgccg
     4621 gcggtgacca ggtcaaacgg ctgtctggac atctccagtt tgcgcgccac ggcgatcacg
     4681 agttcgccca gatagccgcc cgccgcccga agaatctcca atgccgcgga atcaccctga
     4741 aacgctgcat caaagacgag cctggccatg ggttgaaggg cgtccatcga catttcccgc
     4801 cggtacatct tgtagaaaaa ctcgtcgaca tccgagcagc ccgccttctc gacaaacagg
     4861 tctgtgagca gggtcggcgg gcgaacgccg tcccgggcgt ggtagacggc atacagtccc
     4921 tgacggccaa tgatggtgcc ggtccataaa tcgccgaatt cctcgttgag tcctccagca
     4981 cgcgcctcct ggccttgtgg gtttatccct gccgcgatca cgccggtgcc gcaggcgatt
     5041 acgatgccgt atgaggtccg cacgctgccg cgcatggcgg ccatggaatc gttgcggaag
     5101 acccggggcg tgcttccgaa cagcggcgtg tagatttccc gctcaagcat gacgaaatct
     5161 tccggcaggt ccgcccctgc gacgcccatg ccgacccagg cgatatcggc gagcgtgagc
     5221 ccagccgcgt ccagagcatt gcggacggcc tgctggtttt cacgcgccgc ggcagcgatc
     5281 ccctgtacct cgtagctgcc gttgcccgcg cgcccgaaac cgcggatggc agcggtctca
     5341 tccgcaatga ggcagaacgt cttggttgct cccacgtcca ggcccatgta gtatttcatc
     5401 gttgacagtc actctcgttc gattttgcgc cggtcagcgg gagaagcact cccccgcctt
     5461 cgcacatcac gccacgcagg gcgccgtaac agcgcccgga tatctgcctt gaaccattcc
     5521 atactttggg acgcggcgcg cctcacacac aaaagaaaaa gcgcgggatg gaatcccgcg
     5581 cttacacaga actgcaggca acattctgaa ccccagccat gcgctagttg cccttgacct
     5641 cctcgagctt ggagcgagcg cgcgcagccg cgggagtcat cggataattt tcaaccaggt
     5701 cttctaacag ggcgaaagcg cgctcctgct gacccagttt gaggtgtgcc gccgcctggt
     5761 tgaacatgcc atagggaacc ttggcgcttt cggggtgctg cgcgcgcagt gcctcaaact
     5821 ctgcaataca ttcatcgtat cgcccgaggt tgtaaaggca ttcgcctttc cagaactgcg
     5881 cattgtcgcg aatctcactc ttcggaaagc gcgccataaa atcgctgaaa aggttcagcg
     5941 cctcgtcgta ctggtcccgc tggtatgctt ccatggcttt attgtatgcc ggcacggggt
     6001 cgagggcgcc tgccggtgca ggggcagcag cagtggccgc cgcgggctga accgctgctc
     6061 ccatgtccgc cagcgggtcc ggctccgtcg ccagcccgta cgcgggtggc gcggcgacgg
     6121 cggcgctggg cggttcaacc tgaactcgcc cgggcaccac atccgactgg ccaccggcac
     6181 cccatccggt gctggactga ctactggatg gaaccgtgat cccaagcgcc ttatagacgt
     6241 taacggtcag cgaatccagt tttttctcga gttgcgcgag tttctgctgg ttctcttcga
     6301 ccaggccctg gagttcgcgt gactgggtat tggtttcatt gactcgaacg cccaggtcgg
     6361 ttgcggtcac gttcagatgc tcaacggtcc cccccaactc cttctcgaga cgcgacacct
     6421 tgcgatgcat gtcgtagatg gtgtttgcca cagggtcgtt cgggccgccc gtggtagcgc
     6481 acccggcgag cagcagcatt cccagaaggg gggcggcaat catgaaagaa acgcgcatgg
     6541 atgacctctc ttagttcgct ctgttgaact cagcgcgacg gttcttggcc caggcggact
     6601 cagtatggcc cggatccacc ggatcttctt caccatagct gatcgtcacc aggcggtcgc
     6661 ccgaaatgcc gagccggatc aggtagtcgc gcacagccag cgcacgccgt tcgccgagcg
     6721 ccaggttgta ctgctgcgtg ccgcgttcgt cgcagtggcc ttcaatctga atcaccgagt
     6781 tcggcatctg tttgatgatc tctgcattac gtttcagcgt tcccatcgcg gtgggatcca
     6841 attgcgaact atcataggcg aaatagacgg taccggcttc ggccagctgc tggaagaaca
     6901 acttttcctg ctcgaggctg gggagtccct ctccagatgt ggactcggga ccggcgctct
     6961 ggcgcgaagt ggtatcgccg gttagatcgg gggtaatttg ggtcttcttt cttccgcaac
     7021 cgccgacggc gaccgcgacg atgaccaggg ccaacacaaa cagtttcatt gatgttttgt
     7081 acgacatgtc ttcttgttcc tcgctccacc tgacg
//
LOCUS       DAOPBQ010000134         7093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9505_length_7093_cov_4.208440, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000134 DAOPBQ010000000
VERSION     DAOPBQ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9505_length_7093_cov_4.208440"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PKW60_05870"
     CDS             complement(<1..541)
                     /locus_tag="PKW60_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98993.1"
                     /translation="MSTEFSTELALPSPSGSGGGYGERTLSLARLIRLRAPLMLLIFL
                     VLAVPTLTVAYLLVPEEYTASKTIRFAANPVTVLGNESGQGGTVNYDRWVRTQAFEIT
                     DATVMQRVLDDPDIRSLEEIRQAENPLLYLQDLVQARVLGGSENVEVVCTAPNQDLAE
                     RVLAATVKNYMALAVTVAVS"
     gene            complement(551..1864)
                     /locus_tag="PKW60_05875"
     CDS             complement(551..1864)
                     /locus_tag="PKW60_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPJ98994.1"
                     /translation="MTVRAPETIKRGIVMRAKSSWRHDGRLWVFIPAAVACFSIVMLG
                     CASNSKSVVTPRDPKGEGSKIIDESLDIGTAELFGTPGATDSPEPKPRLTAKGQPVPT
                     FEDVDELPYLIEAGDQIEFRCFDDPALSQVAQVRYDGCISLPMIPDIKVQLLTREEAT
                     QLVTEAYSEVFREPQLSLAVITASSKTYHVMGDVMNPQEFPYIKPISVLDAINQAGGP
                     RVNQRGGDSFVGSRSALTQALIIRRSGGTRDVIECDLSKYREPEINPADTLVLPNDIV
                     YVPEGVNLVYVLGETRAPDVYQLMEGTTLLQLLTRAGGFSEPTARMRQVVLIREIEED
                     VNRVFLINVREMLKTGQDMRMKPGDILYIPQKPLVRLQQFVSRFVGSINPLMSLYREA
                     YDTYYTDRRYDELFKRGYSTGTSDLLRTLESIRSFGTLATSIPVP"
     gene            complement(1861..2970)
                     /locus_tag="PKW60_05880"
     CDS             complement(1861..2970)
                     /locus_tag="PKW60_05880"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPJ98995.1"
                     /translation="MNTSLEKVQTRANSIRTPLHEHLIGESKALVETRDVLARLAQSK
                     LTVLITGESGTGKDIAARMLHNLSPRFGRPFIKVNCPAIPESILESELFGYERGAFTG
                     AGNSKPGRFELANNGTIFLDEIAETPYTVQAKLLQVLDGEPFIRIGGVAPIQTDVRIV
                     AATNIKLEDAVRRGDMREDIYYRLNEVHVRMPALRERPEDVPLLAEHFNYHFCAKLGR
                     EYREIDPQHIAEMQAQPWPGNVRELAARVRKYVATGNPELLLREGGPDVEPEVPPARM
                     GRVFEREREPARDLAPAPPRDTRAAEEAPRKFMPLKEAVRKAVEATERTLIEDALRYT
                     LWNRRKAAKLLKISYSSLLRRIEEYHIGKSGSEAE"
     gene            complement(3301..4551)
                     /gene="rho"
                     /locus_tag="PKW60_05885"
     CDS             complement(3301..4551)
                     /gene="rho"
                     /locus_tag="PKW60_05885"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPJ98996.1"
                     /translation="MSIRELKQMTMTELTETARKLNVEGYSGLKKQDLIFKILQQGIE
                     AAGSIYGEGTLEILPDGFGFLRSSDYSYLPGPDDIYVSPSQIRKFNLRTGDTIQGHVR
                     PPKEGERYFALLKVEAVNFESPEVARDRILFDNLTPLHPDERFRLETTQQEVSTRIMD
                     LISPIGKGQRGLIVAAPFTGKTVLLQKIANAVTQNHPEVYVIVLLVDERPEEVTDMQR
                     SVKGEVVASTFDEPHERHVQVAEMVLNKARRLVEHKRDVIILLDSITRLARAYNTIVP
                     ASGKVLSGGVDANALQRPKRFFGAARNVEEGGSLTILATALIETGSRMDDVIFEEFKG
                     TGNMEIHLDRRLMEKRIFPALDVQKSRTRKEELLLEAEELQRIWLLLKILSPLSTIEA
                     TELLIEKLKKTPTNAEFLAMLQKM"
     gene            complement(4848..>7093)
                     /gene="polA"
                     /locus_tag="PKW60_05890"
     CDS             complement(4848..>7093)
                     /gene="polA"
                     /locus_tag="PKW60_05890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPJ98997.1"
                     /translation="AWSGIEQVRERFGVAPERVIDALALIGDASDNVPGVTKIGPVAA
                     KKLLSQYESLEGIYDHIDELKGKQREYLENEREQAFLSRRLVTIDTAVPLDVTLEDLR
                     RRPADHDRLIAVFSELEFQSLVEEFLPQPAAEESSEDYRVIEDDTALRAVVDELRAAG
                     AFAVDTETTSTDPLRAELVGISLCSAPGRACYIPVGHRAPGDGNELEGVAAVSLPHQM
                     DREAALAILRPILEDPAIAKIGHNIKFDLEVLARAGIKLAGITLDTMVASYLTDPSRI
                     RHNLSEVSLHYLKRKMVPITDLIGKGPKQITFDRVPIDIACRYACEDADVTWRLGGRL
                     TTLLRERDLEALFKEVELPLIGVLARMEQAGIAIDADLFRSLSGEVRGRLEALEREIF
                     ELAGEAFVLNSPRQLQHILFTKLGLKPLRRTKTGYSTDVDVLEQLAKEHELPRKMLEY
                     RVLEKLRGTYIDALPKLVNPETGRIHTSFNQTVAATGRLSSSDPNLQNIPVRTGLGKR
                     IRQGFVAESTGMKLISADYSQVELRILAHLSQDPALREAFAQDADIHSDTAARIFGVM
                     PGLVTPDMRYQAKAVNFGVVYGISAFGLARNLGISRAEAARFIDHYFAQYPKVRDWID
                     RTIEEAREHGYVTTLLHRRRPVPDLKSRDVSLRRAAERVAINTPVQGSAADIIKLAMI
                     RLDAALQGSRARLLLQVHDELLVECPASEADGTAATMKRIMEEAFPLAAPLKVDIGIG
                     DNWAEIQ"
BASE COUNT         1336 a         2121 c         2087 g         1549 t
ORIGIN      
        1 cgctgactgc caccgtgacg gccagggcca tatagttctt taccgtcgcc gcaagcacgc
       61 gctccgcgag gtcctgattc ggggccgtgc agacgacctc gacgttttcg ctgccgccca
      121 gcacgcgcgc ctgcaccagg tcttgaaggt acaggagagg attctccgcc tgccggatct
      181 cttccagact ccggatatcg gggtcatcaa gcacccgctg catgacggtg gcatccgtaa
      241 tctcaaaggc ttgcgtgcga acccagcggt cgtaattcac ggtgccgccc tggccgctct
      301 cattgcccag cacggtgacg ggattcgcgg caaagcggat ggtcttgctc gccgtgtatt
      361 cctccggcac cagtagatag gcaaccgtga gcgtcggcac ggccagcacc agaaatatca
      421 gcaacatgag cggggcacga agccgtatca gacgcgccag gctgagtgtg cgctctccgt
      481 accctccgcc ggagcccgac ggtgacggca gagccagctc ggttgaaaat tccgtactca
      541 ttcgagactc ctacgggacc ggaatggaag ttgccagcgt accgaaactg cggatgcttt
      601 cgagcgttct caacaaatcg ctggtgccgg tgctgtaacc gcgtttgaac agttcgtcgt
      661 agcggcgatc cgtgtaatag gtatcgtaag cttcacgata cagcgacatc agtgggttga
      721 tggaaccaac aaaccgcgag acaaattgct gcagacgcac cagcggcttc tgtgggatat
      781 agagtatgtc tccgggcttc atgcgcatgt cttgcccagt cttgagcatt tcacgcacgt
      841 tgatcagaaa aacccgattc acgtcttctt cgatttcgcg gatgagcacc acctgcctca
      901 tgcgggcggt aggctcggaa aatccgccgg cccgggtcaa cagctgcagc agggtggtac
      961 cttccatgag ctggtacaca tcgggcgccc gcgtttcgcc cagcacgtat accaggttga
     1021 ctccctcggg gacatacacg atatcgttcg gcaatacgag agtgtcggcc gggttgatct
     1081 cgggttcgcg atacttgctc agatcgcatt cgatgacgtc gcgcgtgccg ccagaccggc
     1141 ggatgataag ggcctgcgtg agcgcgctgc gcgaacccac gaatgaatcg ccgccgcgct
     1201 gattgacccg ggggccgccc gcctgattaa tggcgtcgag cacggaaatc ggcttgatat
     1261 atggaaattc ctgaggattc atgacgtcgc ccatcacatg ataggtctta cttgacgcag
     1321 tgataacggc cagcgacagc tggggctcgc gaaatacttc cgaatacgct tcggtgacca
     1381 gttgagtagc ctcttcgcgg gtcaggagct gtactttgat atccggaatc atgggaaggg
     1441 agatgcaacc gtcgtagcgg acctgcgcca cctggctgag ggcgggatcg tcgaagcagc
     1501 ggaattcgat ctgatcgccc gcctcgatca ggtacggcag ctcatcgaca tcctcaaagg
     1561 tgggcacggg ctgccctttt gcggtgagcc tcggcttggg ttccggggaa tccgttgcgc
     1621 cgggggttcc gaaaagctca gcggttccga tatccaggct ctcatcaatg atcttgcttc
     1681 cctcgccctt gggatcgcgg ggagtgacaa cggatttgga gttgctggcg catcccagca
     1741 tcacgatgct gaagcatgcc acagccgccg ggatgaacac ccaaagccgc ccgtcgtgtc
     1801 tccaactgct cttggcccgc atgacaatac ctcttttgat ggtttccgga gcgcgtacag
     1861 tcactccgcc tcgctcccac tcttgccgat atggtactct tcaattcgcc gcaacaaaga
     1921 actataacta atcttcaaaa gcttcgccgc cttccgccga ttccacaagg tatagcgcaa
     1981 agcatcctca atcagcgttc tctctgtagc ttccaccgct ttccgtaccg cttccttaag
     2041 cggcatgaac tttcgggggg cttcttcggc cgcgcgcgta tcgcgaggtg gcgctggcgc
     2101 cagatcccgc gccggttcgc gctcgcgctc gaagacccgg cccatgcgag cgggcggaac
     2161 ctcaggctct acgtcgggcc cgccttcacg caacagcaat tcggggttac ccgtggccac
     2221 gtacttgcgt acccgcgccg ccagttcgcg cacattgccc ggccagggct gcgcctgcat
     2281 ttccgctata tgctgcgggt cgatctcgcg atactcccgg cccagtttgg cgcagaaatg
     2341 gtaattaaaa tgctccgcca gcagggggac gtcttccgga cgttccctca aggccggcat
     2401 acgaacatgc acctcattca aacggtaata aatatcttcg cgcatgtccc cgcgccgcac
     2461 cgcgtcctcc agcttgatgt tggtcgcggc gacgatgcgc acgtccgttt gaataggcgc
     2521 tacacctcca atccggataa acggctctcc gtcaagtact tggagtagct tcgcctggac
     2581 agtataagga gtttccgcaa tttcgtcaag aaatattgtg ccgttgtttg ccaattcgaa
     2641 ccggccaggc ttcgaattgc cggcacccgt gaaagccccg cgctcatatc cgaacaattc
     2701 actctccaga atactttccg gaatcgccgg acaattcact ttgatgaacg gccttccaaa
     2761 acgcggggag aggttgtgca gcatgcgggc ggcgatgtct ttgcctgtgc cgctttcgcc
     2821 ggtgatgaga acggtcagtt tggactgagc caggcgcgcc agcacgtccc gtgtttcaac
     2881 cagggcttta ctctcgccaa tcaggtgctc atggagcggc gttcggatac tattcgcccg
     2941 ggtctggacc ttttccaagg aagtgttcac tcgtcgccgc tttccagcag tttgggttaa
     3001 gcgccctcgc catctctgcc gcccaccccg ccgcagtcag agcagaacac gagatatgcg
     3061 caactgctta actcttgaca atattattca ccaaagcaca cgcttttagc aagtctgaaa
     3121 aattcccgac ctcatgcgct ttcttcaagg ccttcaataa cagggattta agagtttttt
     3181 gcctgccagt tcggggctgc tgaatttgat ggatcttgtg taacagccgc caccccgaag
     3241 tcgaatgagt gtcgcttcgg ggtgttggtg cagtgaaagc ctggccgtgc gtggacggct
     3301 ttacatcttt tggagcatcg ccaggaactc ggcgttggtc ggggttttct tgagtttttc
     3361 gatcaacaat tcggtggctt cgatggtact cagcgggctc aggatcttga gcaggagcca
     3421 gatgcgctgc aattcctcgg cctcgagcag gagctcttcc ttgcgggtgc gggatttctg
     3481 cacgtcgagt gcggggaaaa tgcgcttttc catcaagcgg cggtcaagat ggatttccat
     3541 gttgcctgtg cccttgaatt cctcaaagat gacgtcatcc atgcggctgc cggtttcgat
     3601 aagggcggtc gcgaggatgg tcaggctgcc gccttcctcc acattgcgag ctgcgccaaa
     3661 aaagcgtttg ggccgctgga gggcgttggc atcgacgccg cccgacagca ccttgccgct
     3721 ggcgggcaca atggtgttgt acgcgcgcgc gagccgcgtg atcgagtcga ggaggatgat
     3781 cacgtcgcgt ttgtgctcga cgaggcgccg cgccttgttc aggaccattt cggcgacctg
     3841 gacgtggcgc tcgtggggtt cgtcaaacgt ggacgccacg acctcgccct tgaccgagcg
     3901 ctgcatgtcg gtgacttcct caggccgttc gtcgaccagg aggacgatca cgtacacttc
     3961 gggatggttc tgcgtgacgg cattggcgat tttctgaagc agtacggttt tgccggtaaa
     4021 gggcgcggcc acgatcaacc cgcgctgtcc tttgcctatg ggggagatca gatccatgat
     4081 ccgtgtgctg acctcctgct gcgtcgtttc caggcgaaaa cgctcgtcgg ggtgcagggg
     4141 cgtcaggttg tcgaagagga tgcggtcgcg cgccacttcc gggctctcga aattgacggc
     4201 ctcgactttg agcagggcaa aatagcgctc gccctccttg ggaggacgca catggccctg
     4261 gatcgtgtcg cccgtgcgca gattgaactt gcggatctgc gacggagaaa cgtagatgtc
     4321 gtcgggtccg ggcagatagg aataatcgct tgagcggaga aagccgaacc cgtcgggaag
     4381 gatctcgagc gtgccttccc catagatcga accggcggcc tcaatgccct gttgcaggat
     4441 cttgaagatc aggtcttgtt tcttgagccc gctgtatccc tcgacgttga gcttgcgcgc
     4501 ggtctccgtg agctcggtca tggtcatttg tttgagctca cgaatggata tctcgcggtc
     4561 ggagtggtgg atttcctcct cgtggacatc gttttcggcg tcatcgtgca tcgcggcacc
     4621 atttctggct tcgatcgccc gttgagattc gttgtcgctc ttttttacat tacttcgcgg
     4681 ccggcccgcg ggccggacag cgcttttgcg cggggcgcgg cttttggtct tcgaacgatc
     4741 cttcggcgta tcggtttttt ctgccatgcg tcttcctttt gctgcctcgc catgcggctc
     4801 tctccccgag agccagtgag actgcgctga tacgtgctga gtacgggcta ctggatctcc
     4861 gcccagttgt cacctatgcc aatatccact ttgaggggcg ctgccaacgg gaacgcctct
     4921 tccatgatcc gtttcatcgt ggccgccgtg ccgtcggcct cgctggccgg gcattccaca
     4981 agcaattcat cgtgcacctg cagaagcaga cgcgcgcgcg acccctggag ggccgcatcc
     5041 agccggatca tcgccagctt gatgatatcc gccgcgctgc cctgcacggg ggtgttgatg
     5101 gccacacgtt ctgctgcccg ccgcaggctg acgtcccggc ttttcagatc gggcacgggc
     5161 cgccgtcggt gaagcagggt ggtcacatac ccatgctcgc gggcctcttc aatcgtgcgg
     5221 tcaatccagt cgcgcacctt ggggtattgg gcgaaatagt ggtcgatgaa gcgcgcagct
     5281 tccgcgcggg aaatgccaag gtttcgggcc agcccgaacg cgctgatgcc atataccacg
     5341 ccgaaattga cggccttcgc ctggtagcgc atgtccgggg tgaccaatcc gggcatcacg
     5401 ccaaagatac gcgccgccgt atcgctgtga atgtcggcgt cctgcgcgaa cgcttcccgg
     5461 agggcagggt cctgcgacag gtgtgcgaga atgcgtaatt caacttgtga ataatcggcg
     5521 gaaatcagct tcatcccggt ggattccgca acgaacccct gccgaatgcg cttgcccagg
     5581 ccagtccgaa cggggatatt ttgaagattc gggtcgctgc tcgacagccg cccggtggcg
     5641 gccacggtct ggttgaacga cgtatgaatc ctccccgttt cgggattcac caatttgggc
     5701 agcgcatcta tataggtacc ccgaagtttt tcgagtacgc gatactcgag cattttccgg
     5761 gggagttcgt gttctttcgc caactgttca agcacatcga catccgtcga atagcctgtt
     5821 ttcgtacgcc gcaggggctt cagtccaagc ttggtgaaca ggatatgctg gagctgcctg
     5881 ggcgagttga gtacgaacgc ctcccctgcc agctcaaata tttcgcgttc cagcgcttcc
     5941 agccggcccc gcacctcgcc gctcagcgag cggaaaaggt ctgcatcaat cgcgattccg
     6001 gcttgctcca tgcgcgccag aacaccaatg agcggaagct ctacctcctt gaacagtgcc
     6061 tcgagatcac gctcacgcaa cagcgtggtc aagcgccccc cgagccgcca cgtcacatcc
     6121 gcatcttcgc atgcgtaccg gcacgcgata tctatgggga ctctgtcgaa ggtgatctgc
     6181 ttgggccctt taccgatcaa gtccgtaatg ggaaccatct tgcgcttgag gtagtgaaga
     6241 cttacttcac tcagattatg ccttatgcgc gaagggtctg tcaaatagga agcgaccatc
     6301 gtgtccaggg taataccggc cagcttaatt cccgcccggg ccagcacttc caggtcaaat
     6361 ttgatgttgt ggccaatctt ggcaatggcc gggtcttcaa gaatcgggcg tagaatggcc
     6421 agggcggctt cccggtccat ctgatgcgga agggaaactg ctgccacgcc ctccagctcg
     6481 ttgccgtcgc ctggggcacg atgcccaacg ggaatgtagc aggccctgcc gggagccgaa
     6541 cacagtgaaa tgcccacgag ttcggctcgc agaggatcgg tggacgtagt ctcggtgtcc
     6601 acggcaaagg cgcccgcagc gcgcagctca tccacaaccg cgcggagcgc ggtgtcgtcc
     6661 tcaatcacgc ggtaatcttc gctgctttcc tctgccgcag gctgggggag aaattcttcc
     6721 accagggact ggaactcgag ctcggaaaac acggcgatca ggcggtcgtg gtctgccggc
     6781 cgccggcgca ggtcctcgag ggtgacgtcg agcggcacgg cggtatcgat ggtgacgagc
     6841 cggcggctga gaaaggcctg ttcgcgttca ttctccaggt attcgcgctg tttacccttg
     6901 agttcatcaa tgtggtcgta gatgccttcg agcgactcgt actggctcag gagctttttc
     6961 gcggcgacgg ggccgatctt ggtgacgccg ggcacattgt ccgaggcatc gccaatcagg
     7021 gcgagcgcgt cgatcacacg ttcgggagcc acgccaaacc gctcccggac ctgctcaatg
     7081 ccggaccagg cct
//
LOCUS       DAOPBQ010000135         7082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9520_length_7082_cov_4.003131, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000135 DAOPBQ010000000
VERSION     DAOPBQ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9520_length_7082_cov_4.003131"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="PKW60_05895"
     CDS             <1..1133
                     /locus_tag="PKW60_05895"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPJ98998.1"
                     /translation="ELLGRVRTDLDVRRPINECSVAQQQVVEIAKALSFRSKVIVMDE
                     PSATLTDRELTALFDLIRGLKKDGIGMIYISHRLEEIFEIGDRVTVMRDGQYIGTNDV
                     AEVRRENIIHMMVGRELKDEFPKEQFEQGEEVLRVEGLGIAGAFQDVSFSLHKGEIIG
                     LTGLVGAGRTEVARAIFGADRLQSGQIFLDKNPITVSSPQDAIRQGIGLLTEDRKTQG
                     LVLGMTVRENTTLANLNALVKFLFVDRGRERQVTLKYVDDLRIKTPTIEQAAQNLSGG
                     NQQKVVLAKWLFTGCRVLIFDEPTRGIDVGAKVEIYKLMNELVRNGVCILMISSELPE
                     VLGMCDRILVMHEGRLAGELPRAEADQERIMRLATGETLAVR"
     gene            complement(1200..2054)
                     /locus_tag="PKW60_05900"
     CDS             complement(1200..2054)
                     /locus_tag="PKW60_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ98999.1"
                     /translation="MPLRADITGPAQSGPPDPPGWIGAFAGRIFEAWTAGTPMPQFSA
                     AHPEATLADGYAVQRQVVARFAPAHGIGGYKAAGISSAAQAATGIDHPLIGVVPQSGV
                     LSAGQNVVIDLAEYPNRHIETEVGFRFNAPISEPVPDIATLRSLIAHIVAVVELPGGA
                     VEKKHPHTAADTAAWNANGKELITGLETLPPDAVDVDAITVTLTLDGATVSTGRGAEA
                     AGGQMLTLLKCVNSIVEQGYPLEPGHVHTNGAVGTIFPAAPGDYLADFGPLGKIAFAV
                     TDSRIQSE"
     gene            complement(2157..3782)
                     /gene="gpmI"
                     /locus_tag="PKW60_05905"
     CDS             complement(2157..3782)
                     /gene="gpmI"
                     /locus_tag="PKW60_05905"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPJ99000.1"
                     /translation="MSKLSLKKLANYTPFPGPVVLVIMDGVGIGRGDDSDGVHLAYTP
                     VLDELLQEPLYTQLKAHGVAVGLPTDEDMGNSEVGHNALGAGRVFSQGARLVNEAIRT
                     GKIFEGAAWNVIKQRAAAGGTVHFIGLLSDGNVHSHIEQLYALLERCAQDGMTHVRVH
                     PLLDGRDVGEKSAMEYIEPLEARLATLSAGGKDYRIASGGGRMVTTMDRYYADWKVVE
                     RGWKAHVLGEGRQFKSAGEAIQTYYAEDPGITDQYLDSFVIAEDGKPVGTIEDGDAVV
                     FFNFRGDRAIEISMAFEQADFNGFDRKRVPDVFYAGMMEYDGDAHIPRNFLVEPPAID
                     RTIGQYLCAAGVTSYAISETQKFGHVTYFWNGNNSGYIDEALEKYEEIPSDKVTFDQR
                     PWMKAAEITDAVVAQIRQGDRKFIRLNFANGDMVGHTGIPSAIRIAVESVDLGLRRIV
                     PAVRAAKGILIATADHGNADCMWTEKKGKREPMVAHTLNPVPFILKDFSNANTIAMSG
                     VANPGLSNVAATLMNLLGFEKPDDYDPSLITLG"
     gene            complement(3944..4939)
                     /locus_tag="PKW60_05910"
     CDS             complement(3944..4939)
                     /locus_tag="PKW60_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HPJ99001.1"
                     /translation="MRSGCFFLVLAMIAAAAGADGAVPILYTTDLLHPHMDPDDHFDI
                     ATLFGMPEFDIRGVVFDLGPIGKDRPGIVALRQMLHLTGKEVPFATGLLECLSSPDDT
                     GRDQPEETQRGVELILRTLEASSKPMVVFTTGSLRDVAAALNRNGDLCRAKISRLYIN
                     AGHAGGDYEYNVDLDPHAYVRVLNSGLPIYLVPCFGPDGYESYWKFTHREVLDKAPVA
                     IQNFFVYALIQADPTQRDPLKALAEPLSETLREEIWGQERNMWCTGALLHAAGRTSET
                     FSFKEMEVRVEASGRTTLTSSGIGRPMKVFHVDKPGEYRAEMLAVLKEALATFNR"
     gene            5228..>7082
                     /locus_tag="PKW60_05915"
     CDS             5228..>7082
                     /locus_tag="PKW60_05915"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPJ99002.1"
                     /translation="MRTEFAKLLKTIRKYNPDADTELVRSAYRLADQAHKGQMRLSGD
                     PYITHCLAVAANLAQLRLDTTTIVAGLLHDVLEDTRVTNEDLAERFGNEIASLVEGVS
                     NIRALNLPAPLPAELSQQQKQAENLRKMLVATARDVRVILIKLADRLHNMRTIEFLPE
                     DRIRRICQETLDIYAPLANRLGMAHWKWELEDHAFHHLNPAMYREMAARVAMKRRERE
                     AWLKDTVEFLEQRLTEAEVEARVVGRPKHLYSIYQKIIQQGKDFDQVMDILAVRIITQ
                     TVAGCYNALGVVHQLWPPVPGRFKDYIAVPKINMYQSIHTTIMRENGLPLEIQIRTEE
                     MDRTAREGIASHWRYKEGLGKIDPKLDSRLRWLRQMYEWLNEAHVPEELLDSVRRDVG
                     ATEVYVFTPKGEVKELPGGATPLDFAYMVHSDVGHHCIGARVNSAMVPLRYHLQTGDV
                     VEILTSKNQTPHLDWLNIVVTGKARTRIRQRLREMGELEPLEGQEPAAKAPLRAPRKP
                     ASTIRPVDDETRHKLVRVQGNKAIAVHFAKCCNPMPGHPIIGYITKGQGITVHRAECS
                     HFSVTKRDPDRTVEAHWDGVLGGVTREVGMRVTIGPRPNVLADITNAIRPMN"
BASE COUNT         1414 a         2221 c         2107 g         1340 t
ORIGIN      
        1 gcgagctgct cgggcgcgtc cgcaccgacc tcgacgtccg ccgccccatt aacgagtgca
       61 gcgtggccca gcagcaggtg gtcgagattg ccaaggccct ctcgttccgc tcaaaggtca
      121 tcgtgatgga cgagccctcc gcgacgctga ccgaccgcga actcacggcc ctgttcgatc
      181 tgatccgcgg cttgaaaaaa gacggcattg gcatgatcta catctcccac cggctggagg
      241 agatcttcga gatcggcgac cgggttaccg tcatgcgcga cggacagtat attgggacca
      301 acgatgtcgc cgaggtgcgc cgggaaaaca tcattcacat gatggtcggt cgtgaactga
      361 aagacgaatt cccgaaagag cagttcgaac agggtgagga agtcttgcgc gtcgaaggcc
      421 tgggtatcgc cggcgcgttt caggatgtga gtttcagcct gcacaaaggc gagatcattg
      481 ggcttaccgg acttgtgggc gccgggcgca ccgaggtcgc gcgcgccatc tttggcgctg
      541 accggctgca gagcggtcaa atcttcctcg acaagaaccc gatcaccgtg tcgtcgcccc
      601 aggacgccat ccgccaggga atcggattgc tcacggagga tcgcaagacg cagggcctcg
      661 ttctgggcat gaccgtccgc gaaaatacta ccctcgccaa cctcaacgcg ctggtcaaat
      721 ttctgttcgt ggaccgcggg cgcgagcgcc aggtgacgct gaagtatgtc gacgacctac
      781 gcatcaaaac gcccaccatc gagcaagccg cgcaaaatct cagcggcggc aaccagcaga
      841 aggtcgtgct ggccaaatgg ctgtttaccg gatgccgcgt gctcatcttt gacgaaccca
      901 cccgcggcat tgacgtcggc gcgaaggtgg aaatctacaa gctgatgaac gaactggttc
      961 gcaacggcgt gtgtatcctc atgatctcaa gcgaactgcc tgaggtcctg ggcatgtgcg
     1021 atcgcatcct cgtcatgcac gagggacggc tggccggtga actgccccgg gccgaagcag
     1081 accaggagcg catcatgcgc ctcgcgacag gagaaacgct cgccgtgcgg taacccgcgg
     1141 ccgtgccttc tgcggcaggc agcgccggaa aaaagcgggc aggcagactc ccgtgcgcct
     1201 tactccgatt gaatccgcga atccgtgacg gcaaaggcaa tcttgccgag cggaccgaag
     1261 tcggccagat aatcgccagg cgccgcgggg aaaatcgtcc cgaccgcgcc gttggtgtgc
     1321 acgtggccgg gctcaagcgg gtatccctgc tccacgatgc tgttgacgca tttgaggagc
     1381 gtcagcatct ggccgccggc agcctcggcg cctctgccgg tgctgacggt cgcgccatcc
     1441 agggtcagcg tgaccgtgat ggcatcgacg tcaaccgcgt ccggcgggag cgtttccaat
     1501 cccgtgatga gttctttgcc gttggcgttc caggcggcag tatcggcggc ggtatgggga
     1561 tgtttctttt ccaccgcgcc gccgggcagc tcgactacgg ccacgatatg tgcgatgagg
     1621 ctgcgcaggg ttgcaatatc cggcacgggc tcagaaatgg gcgcgttgaa gcggaaacct
     1681 acctccgtct cgatgtggcg gttgggatac tcggcgagat cgatcaccac gttctgcccg
     1741 gcgctaagca cgccggactg cggcaccacg ccaatcagcg ggtggtctat gcctgtcgct
     1801 gcctgcgccg cactgctgat ccccgcggcc ttgtatccgc cgattccatg cgccggggcg
     1861 aagcgcgcca ccacctgcct ttgaaccgca tacccgtcgg ccagcgtagc ctcggggtgc
     1921 gccgcgctga attgcggcat gggcgtaccg gcagtccaag cctcaaatat gcgcccggcg
     1981 aacgcgccga tccatccggg cggatccggt ggaccggact gggccgggcc cgtaatatcc
     2041 gcacgcagcg gcaaacgggc aatcctcttt cggattggac gccgggacgc gtcctggtga
     2101 tctctggaac tggacatagg cctgcctccc aggacccgtg ccgcgcaggc gcttgctcag
     2161 cccagcgtga tcagcgacgg atcgtaatcg tccggtttct cgaagcccaa caggttcatc
     2221 agggttgccg ccacgttgct caacccggga ttggccacgc cgctcatggc gatcgtgttt
     2281 gcgttcgaga agtctttgag aataaacggc accggattca gcgtgtgcgc caccatgggc
     2341 tcacgtttgc ccttcttctc ggtccacatg cagtcggcgt ttccgtggtc ggcggtggcg
     2401 atcaggatgc cctttgccgc acgcaccgcc gggacaatac ggcgcaggcc cagatcgacc
     2461 gattccaccg cgatccgtat cgcgctggga atgccggtgt ggccgaccat gtcgccgttg
     2521 gcgaaattga gccggatgaa cttgcggtct ccctgccgga tctgagccac gaccgcatcg
     2581 gtgatctccg ctgccttcat ccagggccgc tgatcaaacg tgaccttgtc ggacgggatt
     2641 tcctcatatt tttcgagcgc ttcgtcgatg tatccggaat tgttgccgtt ccagaaatag
     2701 gtcacatggc caaatttctg agtctcggag atggcatagg aggtgacgcc cgcggcgcaa
     2761 aggtattgcc cgatggtccg atcaatcgcg ggcggttcca caagaaaatt ccgcgggatg
     2821 tgggcgtcgc cgtcgtattc catcatcccc gcataaaaca cgtcgggcac gcgcttgcgg
     2881 tcaaatccgt tgaaatccgc ttgttcgaag gccatggaga tctctatggc ccggtcgccc
     2941 cggaaattga aaaacaccac ggcgtcgccg tcttcaatgg tcccgacggg cttcccatct
     3001 tccgcgatga cgaagctgtc gagatactgg tcggtgatgc cgggatcttc ggcgtagtag
     3061 gtttggatcg cttcgcccgc agacttgaac tggcgccctt cgccaaggac gtgcgctttc
     3121 cagccgcgtt ctacaacctt ccagtcggca taatagcggt ccatggtggt gaccatgcgg
     3181 cccccgcccg aggcgatgcg gtaatctttg ccgcctgcgc tcagggtggc caagcgcgct
     3241 tcgagcggct cgatgtactc catcgcgctc ttttcgccca cgtcgcggcc gtccagcagc
     3301 ggatgcaccc gcacgtgcgt catgccatcc tgcgcacaac gctccagcag ggcgtacagc
     3361 tgttcgatat ggctgtgaac attgccgtca gacagcagtc cgatgaaatg gaccgtgccg
     3421 ccggcggcag cacgttgctt gataacattc caggccgcgc cttcgaagat ctttcccgtg
     3481 cggatggcct cattcaccag ccgcgcaccc tgcgagaaca cccggcccgc gcccagggcg
     3541 ttgtgtccga cttcggaatt gcccatgtct tcatcggtgg gaagcccgac cgccacgcca
     3601 tgggccttca gctgcgtgta gagtggctcc tggagcaatt catcgagaac gggcgtgtag
     3661 gccaggtgaa cgccgtcaga gtcgtcgccc cggccaatgc ccacgccatc catgatgacc
     3721 agcaccaccg gtccgggaaa aggggtgtaa ttcgccagtt tcttcaggct cagtttgctc
     3781 ataagtcgtc cctgtcgtga tggtttcgtc cggaaggcgt aagtgtaccg ggcgcgcggg
     3841 ttccgttcaa atctgcggcg aaaaccgcgg tgttttcaga cagtagaaag gcctgcgggc
     3901 gccgggcgtc tacaagccgc attcgccggc gattccctgg ccctcagcga ttgaatgtcg
     3961 ccagcgcctc tttcaagacg gcaagcatct ctgcgcggta ctcgcccggc ttatccacat
     4021 ggaacacctt catgggccgg cctatgcccg aagaggtcag cgtggtgcgt cccgacgctt
     4081 cgacccgcac ctccatttcc ttaaaagaaa acgtctcgct cgtgcggcct gcggcatgca
     4141 gcagcgcccc cgtgcaccac atgttccgtt cctggcccca aatctcctcg cggagggttt
     4201 cggacagggg ttccgccagg gctttcagcg gatcgcgttg cgttgggtcg gcctggatca
     4261 gggcatagac gaaaaagttc tggatcgcca cgggtgcttt atccagaact tctctatgcg
     4321 tgaatttcca gtacgactca tatccatccg ggccaaagca cggcacgagg tatattggaa
     4381 gcccgctgtt caacacgcgg acataggcat gcgggtcgag atcgacgtta tattcgtagt
     4441 cgccgcccgc atggcccgca ttgatgtaca ggcggctgat cttcgcccgg cacaaatcgc
     4501 cattgcggtt cagggcggct gccacatcgc gcagactgcc tgtcgtgaac accaccatgg
     4561 gcttggaact ggcctccaga gtgcgcagga tcaactctac cccgcgctgt gtctcctccg
     4621 gctggtcacg gcccgtgtcg tctggcgagc tgaggcattc aagcagtccg gtcgcgaatg
     4681 ggacctcttt tccagtcaga tggagcatct gccggagcgc cacgatgccc ggacgatcct
     4741 tgccgatggg cccgagatcg aatactacgc cgcggatatc aaactccggc atcccgaata
     4801 aggtcgcgat atcgaaatga tcatccggat ccatatgcgg atgcaggagg tccgtggtat
     4861 aaagaatcgg cacggcccca tcagcgccgg cagcggcggc aatcatagcc aggacaagaa
     4921 aaaaacaccc acttcgcatc gatttcctcc ttcatgttcc cgcatgacta tgcccaacgg
     4981 gcagggattg ttaaagtacc ccgaaccatg ccctgaaacg cgtgccccgc acggcttaac
     5041 acggccaaag tgcatctgag caggtcagat cagccctcct tgcgccatcc cgccttccat
     5101 gccccggtgc ggttttgagt ggcgcgattc ggcgaattat gaacgatttg tggcaaaatg
     5161 gtactatatg cgtctgacga gcctgcgccc gaaaccgccg gacgcctgaa tccgcagttg
     5221 aggccacatg cgtaccgaat tcgctaaatt gctgaaaacc atccggaaat acaatccgga
     5281 tgccgacacg gagctcgtcc gtagcgccta tcgccttgcc gaccaggcgc ataagggaca
     5341 gatgcgcctc tccggcgacc cgtatatcac gcactgcctg gccgtggccg ccaatctggc
     5401 ccagctccgg ctcgacacga ccacaatcgt cgccggcctg cttcatgatg ttcttgaaga
     5461 cacacgggtt acaaatgaag acctggcgga gcgcttcggc aacgagattg cctccctcgt
     5521 ggagggtgtg agcaatattc gcgccctcaa tctccccgca ccactccccg cggaactctc
     5581 ccagcagcag aaacaggccg aaaacctccg taagatgctg gtcgctacgg cccgtgatgt
     5641 gcgggtgatc ctgatcaagc tggcggaccg cctgcataac atgcgcacca ttgaattctt
     5701 gccggaagat cgaatccggc gcatttgcca ggagaccctg gacatttatg ccccgctggc
     5761 gaatcggctg ggcatggcgc attggaaatg ggaactcgaa gaccacgcat ttcaccacct
     5821 gaacccggcc atgtaccgcg aaatggcggc gcgcgtggcg atgaagcgcc gggaacgcga
     5881 ggcgtggctg aaagacaccg tagaattcct cgaacagcgg ctcacggaag ccgaagtgga
     5941 ggcccgggtc gtcggccgtc ccaaacacct ctacagcatc taccagaaga tcatccagca
     6001 gggcaaggat ttcgatcagg ttatggacat cctggcggtg cgcatcatca cgcaaaccgt
     6061 tgcgggatgt tataacgcgc tgggtgtggt gcatcaattg tggccgcctg tgccggggcg
     6121 cttcaaagac tacatcgccg tgcccaaaat caacatgtac cagtccatcc acacgacgat
     6181 catgcgggaa aacggactgc ccctcgaaat tcagatccgg accgaggaga tggatcgtac
     6241 cgcccgcgaa ggcatcgcgt cgcactggcg gtataaagaa ggactcggga aaattgatcc
     6301 caagctggac agccggttgc gctggctccg gcagatgtac gaatggctca atgaagcgca
     6361 tgtcccggaa gagcttctcg acagcgtccg gcgcgatgtc ggtgcgacgg aagtatacgt
     6421 cttcactccg aaaggcgagg tcaaggagtt gccagggggc gcgaccccgc tcgattttgc
     6481 ctacatggtc cactccgatg tgggccacca ctgcatcggg gcgcgcgtca acagcgccat
     6541 ggtgccgttg cggtaccatc tccagacagg tgatgtcgtt gaaattctca cctcgaaaaa
     6601 ccaaacgccg catctggatt ggctgaacat cgtcgtgacg ggcaaggcgc gcacgcgtat
     6661 ccggcagcgt ctgcgggaaa tgggcgagct cgagccgctg gaaggtcagg aacccgcggc
     6721 caaagcgcct ctccgggcgc cccgcaagcc cgcaagcaca atccgcccgg tcgacgatga
     6781 aacccgccat aaactggtgc gcgtccaggg caacaaagcc attgcggtgc attttgccaa
     6841 gtgttgtaat cccatgcccg gccatcccat tatcggctac atcacgaaag gccagggcat
     6901 caccgtgcat cgggccgaat gctctcattt cagcgtcacc aagcgcgacc ccgatcggac
     6961 cgtcgaagcg cactgggatg gcgtcctggg cggcgtgacc cgcgaagtgg gcatgcgcgt
     7021 taccattggc cctcgtccca acgtcctggc cgacatcacc aacgctatcc gtcccatgaa
     7081 ca
//
LOCUS       DAOPBQ010000136         7077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9533_length_7077_cov_3.545144, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000136 DAOPBQ010000000
VERSION     DAOPBQ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9533_length_7077_cov_3.545144"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..1014)
                     /locus_tag="PKW60_05920"
     CDS             complement(310..1014)
                     /locus_tag="PKW60_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPJ99003.1"
                     /translation="MHARMGIAALTIVLAAALPAGAINIPNPTPHAAELSVYEVYNSL
                     YGTSFTSNVDLDVQRVVDLQTFLLPFGSDLYVEARARYASTVSEFGYYTPSGLPAVQT
                     PLFSVTAVGDVSGAGFNAVINTSDPFGFYMDPAGPAFWYSEQALNPFGEDHMVAYSVA
                     GDPSLLLLAWEDLTLPSTQGQLPRPVAGPAPDGDYNDLIVELRLREIVPEPASLLLFG
                     MGIAGMGVVRIRHFFS"
     gene            complement(1293..2705)
                     /locus_tag="PKW60_05925"
     CDS             complement(1293..2705)
                     /locus_tag="PKW60_05925"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPJ99004.1"
                     /translation="MTYQGLSTTEIGPERGGAVVVLDTDPGVRWALEKGLKRSGYDVR
                     TASTSGEALRILYAESVRAVIMEILPEADLTPDVLTTVLESPSKPKVICVSMESEPKA
                     IIECVRRGAADFVTKPFNLNDVRAVLRRALASQSEQRTLRRPGSGRAGMETSYLIGVS
                     HALQELRTIIHQVAHTDLNCLIRGESGTGKDLVAREIHRLSARRDRPFVKVNCSALPE
                     QLLESELFGYEKGAFTGALSSKPGRFELANNGIIFLDEIGEMYPNLQAKILQVIEHKE
                     FTKLGGRKHIRVDVQIIAATNADLEVKTKEGSFRHDLYFRLNEVCIWVPPLSARKEDI
                     PLLVRHFVQKHGGFGVGEAVDISGDELARLIDQPWPGNVRELESTVKRWIALGDSAIT
                     FSRRSLEREPDVGTEQAQPPVEPPPPPIPVQTQRSEVERILDALEKHKWNRRKAAEEL
                     GISYQTLRRRIDKLQLDRRR"
     gene            complement(2702..4387)
                     /locus_tag="PKW60_05930"
     CDS             complement(2702..4387)
                     /locus_tag="PKW60_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPJ99005.1"
                     /translation="MAGRNQVQVNGVQKPGILIIEDEPTQLAFLEEILKEWDYECVTG
                     GSIAEGRTLFEKDRFACALIDLGLPDGNGLSLISEFAQADPCLVPIVLTGARQTETVI
                     DTMREGAFDYLVKPVDAATLKAALTRALSHHSVVRERGELFALLLEEREQLRARVEEA
                     TEDIRQYASACERSNTRLQALLRLSRFASGYFSEEALLRGLFAELGEHIPLRAVVICD
                     ISSERLIGLYQSGQEESAYIETSTSLELEESGALLIEAEPELLLQRWLDRNTDLVGIA
                     LRSFVFSQQQQNTMRYIVGFFTEPAFGQLQGDREFLDTCARFLVFEWEQAKLLLHVAH
                     QASLGNIGIEVARNFIQPLTAIRIAADIVQEAAISSDAIEGVHVILENVSKLHRQTQE
                     FRKLALLREGSVETVRLDEFIEHALDVLAVAIQNRRVTVNRQFETDSECVLLNGTTLA
                     RTFLDLILDAVRSVDAGAEIVLALRRADTEHLVFEMRYESTSTNFQASLATLNSTLAP
                     REKGNPGLQLAQRTVHSCGGNLSFDLEDDRRARLRIVLPLNATRVVTHREVLQ"
     gene            4681..5256
                     /locus_tag="PKW60_05935"
     CDS             4681..5256
                     /locus_tag="PKW60_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPJ99006.1"
                     /translation="MVVEDMKELKAAIDDLQTRLDDEARMRSELERRCRVLEKLAYRD
                     PGTGLRTEPYLHERVREEIERSIRYPSTATLLTFCTPRANPDMMTRLGLRLTDDLRMT
                     DQVFTLSEGGLAVLLVETPEEGAHKVLHRLKAELEQVLVSFGYSLTSFPVDANLAEDF
                     LNLAMERHNTVARQMYMSNDSSTPVAPTAVH"
     gene            5835..6506
                     /locus_tag="PKW60_05940"
     CDS             5835..6506
                     /locus_tag="PKW60_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99007.1"
                     /translation="MKYVMFTLLCAAMLFGVMAVPAYADQEKAPLKIELPEKSFMGTP
                     LDYWSENLEFTYKARDPFMAPVGTELVSKGKPVTSSDKSPSMGKLPMITDGDKGFQEK
                     SLVELDKGLQWVQIDLQKKHKIYAILVWHFHTSERVYHDFVVRVANDPDFTQDVVTLY
                     NNDHDNSAGLGAGTDKEYVESNEGRLVDGKGAEARYVRLYSKGNTSNERNNYVEVEVW
                     GIPSE"
     gene            6690..>7077
                     /locus_tag="PKW60_05945"
     CDS             6690..>7077
                     /locus_tag="PKW60_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99008.1"
                     /translation="MEDLGAHDQKRLAWWFVAGYAAVMLLAAAYRVPHLGERVFHGDE
                     AVQAFKAGQELYEHGDYAYDPEEYHGPTLYYLTVPALWLAGIERFADSAEVHYRIVPV
                     LFGLGIVACVWWARDALGRRAALIAGL"
BASE COUNT         1405 a         2004 c         2213 g         1455 t
ORIGIN      
        1 ccttggcata tgcagtgtaa gaccaatatt tgtaacagct tacgttgtat ttggcggggc
       61 ctggagagca aaccagcggg caagctgtgt tcgcttgttg ttcaacattg agtaactatt
      121 aattcaaaag accttaaaaa ttctgtctgt ttacaattaa atagatgcac agaacaatag
      181 aatgctgatc catgatttga aagtctcaca aacgctctac actgcagaaa aattcatctg
      241 gcccgaaacg gccatccatt tcgcgccaga gattcgatgt ttggctggtt gcctcagctt
      301 cgcgtgggct cagctgaaga agtgacgtat gcggactact cccatgccgg cgatccccat
      361 gccgaagagg agaagcgagg ccggttcggg gacgatctca cggagccgga gctctacgat
      421 gaggtcgttg tagtcgccgt cgggggccgg accagcgacg ggccgcggaa gttgaccctg
      481 cgtggagggc agagtcagat cttcccacgc cagcaacaga aggctggggt ctcccgcgac
      541 agagtaggcg accatgtgat cttcaccgaa cggattcagt gcctgttcgg agtaccagaa
      601 tgccgggccg gcgggatcca tgtagaaccc gaatggatcg ctggtgttga tcacggcgtt
      661 aaaaccggcg ccggagacgt cgcccacggc tgtcaccgaa aacagcggag tctggacagc
      721 cggcagacca cttggcgtat agtacccgaa ttccgatacg gtcgacgcgt agcgggcccg
      781 cgcctccacg tacagatcgc ttccaaaagg aagcaggaag gtctgtagat ccacgacgcg
      841 ctggacatcc aggtcgacgt tactggtgaa tgacgtgcca tagaggctgt tgtagacctc
      901 gtacacgctc agttccgccg cgtgtggcgt cggattcgga atgttgatgg cgcctgccgg
      961 aagcgccgca gccagcacta tggtgagtgc cgcaataccc atcctcgcat gcataaccgt
     1021 tctcccttgt cgcggtcgca cagcgccgct gtttgctggc taatcactca gtcttcccta
     1081 cccacgtgtg cgccccgata cttcccgcat aatggatgaa aggaggtttt tagccacgcc
     1141 ctctctcgca gactaacgac agggcaaaca tggcatttgg cggctctatt ccgggaagat
     1201 ccgcttaatt tggcgtgttg cgctgcgccg ggttatctcc gcaaccgcgg caggaaaacc
     1261 ccggatttga tgcggaatgt acggggcggc gcctagcggc gccgatcaag ctggagtttg
     1321 tcgatacgcc tgcgaagggt ctgatagctg atgccaagtt cttccgctgc tttccggcgg
     1381 ttccacttgt gcttttcgag ggcgtccagg atacgttcaa cctcggaccg ctgcgtctgc
     1441 acggggatcg gggggggagg cggctcgacg ggcggctggg cctgctcggt cccgacgtcc
     1501 ggttcgcgtt ccagcgaacg cctgctgaag gtgatcgcac tgtcgccgag ggcgatccag
     1561 cgcttgacag tgctttcgag ttcgcgcacg ttgccgggcc agggttgatc gataaggcgg
     1621 gcgagttcgt cgccggagat gtccacggct tcgcccaccc cgaaaccgcc gtgtttctga
     1681 acaaaatgac ggacgagcag agggatgtct tctttgcggg cagacagcgg aggcacccag
     1741 atgcagactt cattgagccg gaaatagagg tcgtggcgaa agctgccttc tttggttttg
     1801 acctcgagat ccgcgttggt ggcggcaatg atctgcacgt cgacgcgaat gtgttttctg
     1861 ccgcccagct tggtgaattc cttgtgctcg atgacctgca ggatcttggc ctgaaggttg
     1921 ggatacattt cgccgatctc gtcgagaaag atgatgccgt tgttggcgag ttcaaagcgg
     1981 cccggtttgg acgacaaggc tcctgtgaag gcgccttttt cgtagccgaa caattcgctt
     2041 tcgagaagct gttccgggag tgccgagcag ttcaccttga cgaacggccg atcgcgccgg
     2101 gcggacagcc ggtggatttc gcgcgcgacc aggtctttgc cggtgccgct ttcgcctcga
     2161 atgaggcagt tgagatcggt atgcgcaacc tgatggatga tggtgcgcag ttcctggagt
     2221 gcgtggctta cgccgatcag atacgacgtc tccataccgg ccctgccgga gccggggcgg
     2281 cgcaaggtgc gctgctcaga ctggctggcc agggcgcggc gaagcacggc ccgaacgtcg
     2341 ttcaagttga agggtttggt cacgaaatcc gccgcaccgc ggcgcacgca ctcgatgatg
     2401 gctttgggct cggattccat cgagacgcag atcaccttgg gttttgacgg tgattcgagg
     2461 acggtggtca acacatccgg cgtgaggtcg gcctcgggga ggatctccat gataacggcg
     2521 cgtaccgact cggcgtagag aatacgcagg gcttcgcccg aggtactggc ggtgcggacg
     2581 tcatagcccg agcgtttcaa gcctttttcg agcgcccagc gaacaccagg gtcggtatcg
     2641 aggacgacga ccgcgcctcc gcgctccggt ccgatctcgg ttgtggacag gccctggtat
     2701 gtcactggag cacctcgcgg tgcgtgacca cgcgggttgc attgagcgga aggacgatac
     2761 gcaggcgcgc acgcctgtca tcttcgaggt cgaacgagag atttccgccg cagctatgga
     2821 cggtgcgctg ggccagctgg agccccgggt tgcctttttc cctcggcgcc agcgtcgaat
     2881 tcagcgtcgc cagagaggcc tgaaagttgg tcgaggtgct ctcgtaacgc atttcgaata
     2941 cgagatgctc ggtgtcggca cggcgcaggg ccaggacgat ctccgcgccc gcatcgaccg
     3001 accgcacggc atccagaatc aagtcgagaa aggtgcgggc gagggtcgtt ccgttgagga
     3061 ggacgcattc gctgtcggtt tcgaactggc ggttcacagt gacccgccgg ttctgaatgg
     3121 cgacggccag cacgtcgagg gcgtgctcga tgaattcgtc gagtcgaacg gtttcaaccg
     3181 agccttcgcg cagcagggcg agcttacgga attcctgagt ttggcggtgc agcttgctca
     3241 cgttttccag gatgacgtgc acgccttcga tggcatccga cgagatcgcg gcctcctgca
     3301 cgatgtcggc ggcaatccgg atggcggtga gcggctgaat gaaattgcgg gccacttcga
     3361 tgccgatatt gccgaggctt gcttgatggg ctacgtgcag caggagtttg gcctgttccc
     3421 attcgaagac cagaaaccgg gcacaggtgt ccagaaattc gcggtcgccc tgaagttgcc
     3481 cgaacgccgg ttcggtaaag aacccgacga tgtaccgcat ggtgttctgc tgctgctggg
     3541 aaaacacgaa ggaacgcaag gcgatcccga cgaggtcggt gttccggtct agccaccgct
     3601 gcaggaggag ctcgggttcg gcttcaatca gcagggcgcc gctttcttcc agctcaagcg
     3661 atgtactggt ttcaatatac gccgattcct cctgaccgct ctggtacagg ccgatcaggc
     3721 gttccgagga aatgtcgcaa atcaccaccg cgcgcagcgg aatgtgctcg cccagctcgg
     3781 caaacaaccc gcgcagcagg gcttcctcgc tgaagtatcc cgacgcgaac cggctcagcc
     3841 ggagcagggc ctgcaaccgg gtgttgctgc gttcgcatgc ggacgcgtat tgccggatgt
     3901 cctcggtggc ctcttccacg cgggcgcgca gttgttcgcg ttcttcgagc aacagggcga
     3961 aaagctcccc gcgttcgcgt acgacggagt ggtgcgacag cgcgcgcgtc agcgcggcct
     4021 tcaacgtagc ggcatcgacc ggtttgacga ggtaatcaaa agcgccttca cgcatggtgt
     4081 caatcacggt ctcggtctgc cgcgcccccg tgagaacgat cggcacaaga caggggtcgg
     4141 cctgggcaaa ctcgctgatg agcgacagac cattgccgtc gggcaggcca agatcaatga
     4201 gtgcgcaggc aaaccggtct ttctcgaaaa gcgtccggcc ctcagcgatc gagccccccg
     4261 tgacgcactc gtaatcccat tccttcagga tttcttcgag aaacgccagc tgggtaggct
     4321 catcttcgat gatgaggatc cccggtttct gtaccccatt gacctgcacc tgattccttc
     4381 ccgccataca ccacgggcag cccgcccccg gccagcaacc ttcctgcttt gccactacat
     4441 gctacctgac ggcgtgattt ctgtcaagaa ttaaatgcct attacagtca ggataccttt
     4501 aactcataat gaaaaagtgg tcaaaactga caaccttcag cgcattctgg caaatacttt
     4561 gatccgctcc agcctttctt aaaagtcgca aaaggtaacg cactatacga cttagcgcgt
     4621 tgcggccttg aatttgcgca tttggcacat attatgctct gcctcatgtg gagaggagtt
     4681 gtggtagtgg aagatatgaa agaattgaaa gcggccatcg acgaccttca aacacgtctc
     4741 gatgacgagg cgcgtatgcg gtccgaactc gagcgccgat gtcgtgttct ggagaaactg
     4801 gcctaccgcg acccgggaac gggcttgcga accgaaccct acctgcacga gcgggtccgg
     4861 gaagagatcg aacgatcgat ccgctatccc tcaacggcca cgctgctgac cttctgcaca
     4921 ccgcgcgcca atcctgacat gatgacccgc cttgggctcc gcttgacgga cgatcttcgc
     4981 atgacggacc aggttttcac gttgagcgag ggcggtcttg cggtgcttct tgttgaaaca
     5041 cccgaggagg gagcgcataa ggtgctccac cggctcaaag cggaactcga gcaggtgctc
     5101 gtgagcttcg gttattctct tacgagtttc cccgtggatg ccaatctggc ggaagacttt
     5161 ctgaatctgg ctatggagcg gcacaatacg gttgcgcgcc agatgtacat gagtaatgac
     5221 agctcgaccc cggtcgcacc caccgcggtt cactgactcc tgcgcaatcc gtgcgtgccg
     5281 cgggggagcg gcaggtgccg caagtctctg tttttccgcc gtcttctcgc aggggatggg
     5341 cgcctctgcc gcattgcagg gcgccaggcg agacctggct gatttcgctg tacgcaggcg
     5401 cacggacgcc ccctgcgtgg ccgcccatcc agatagcccc acgcggtgga cctgaaggag
     5461 ggcaggcaca ggggcctgcc cctgcgcgct gcgaaaaccg ggattggcca gggcaggcgc
     5521 gacaagtttc gcgtcttgtg ccccccggcc tcttttttcg gttgcgtgct caaggctttc
     5581 caaaaatcgc tcttgtgaca ccgccctggc tttcatgttc gggcacgatc ccagaaggca
     5641 cgacgatacc gtccccgaca aaagcccaca tggcgcggta cgttgatttg actttgcggg
     5701 cctgaaaaaa ggatactctt cgggtgtgca atgtgaaggc tttgcggaac tttgtgtgaa
     5761 tacacgggtc cgttctgcgt cttttatccg ggaacaccat tgggctgtac gccttgaatt
     5821 cgaggaggtt acgaatgaaa tacgtgatgt ttacccttct gtgcgcggcg atgctgttcg
     5881 gagtgatggc ggtgccggct tatgcggacc aggagaaggc gcctctgaag attgaattac
     5941 ccgagaagtc gtttatgggc acgccgctgg attactggtc ggaaaacctg gaattcacgt
     6001 acaaggcgcg tgatccgttt atggcgccgg tgggcaccga actggtgtcg aaaggcaagc
     6061 cggtgaccag cagcgacaag agcccgtcga tgggcaagct gcccatgatc accgacggcg
     6121 acaagggctt tcaggaaaag agcctggtcg aactcgacaa aggccttcag tgggtgcaga
     6181 tcgacctgca gaaaaagcac aagatttatg caatcctggt atggcacttc cacacctccg
     6241 agcgggtgta ccacgatttt gtggtgcgcg tggccaatga ccccgatttc acgcaggacg
     6301 tggtgaccct ctacaacaat gaccacgaca attccgccgg cctcggcgcg ggcacggaca
     6361 aggaatacgt ggagagcaac gaaggccgcc tggttgacgg caagggcgcg gaagcgcggt
     6421 atgtgcgcct gtacagcaag ggcaacacct cgaacgagcg caacaattac gtcgaagtgg
     6481 aagtctgggg gattccgtcc gaataaggcc ccggctttta atcaaccgga gcaatagcgc
     6541 atactcgtcc cgccgttgcg ccagtgcgcg tgacggcggg acttttgttt cagggggagt
     6601 acagcacgcc cggactgttt tgtacggtac gtaattgccc tgactccggc ggccggccgc
     6661 cgggagcgcg ggcgggtgac agcaggatta tggaagatct tggggcacac gatcagaagc
     6721 ggcttgcgtg gtggtttgtg gcgggttatg cagcggtcat gctgctggcg gcggcgtacc
     6781 gggtgccgca cctgggcgaa cgcgtgttcc acggcgacga ggcggtacag gcattcaagg
     6841 ccggtcagga gctctacgag cacggcgact acgcgtatga tccggaggag tatcacgggc
     6901 ccacactgta ttacctgacc gtgcccgcct tgtggctcgc cggcattgaa cggtttgctg
     6961 attcggccga ggtgcattac cgcattgtgc cggtgctgtt cggtttgggg attgtggcgt
     7021 gcgtctggtg ggcccgcgac gcgctgggac ggcgcgcggc gctgattgca ggcctgt
//
LOCUS       DAOPBQ010000137         7076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9535_length_7076_cov_4.248398, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000137 DAOPBQ010000000
VERSION     DAOPBQ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9535_length_7076_cov_4.248398"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PKW60_05950"
     CDS             complement(<1..920)
                     /locus_tag="PKW60_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99009.1"
                     /translation="MKPILALFLTLAALAIPLAWGETEVLQGTSKGDLESNLSIFNLF
                     ETRPEPVQRFYAAAYGILAADPRAAFLEMTEDPEIQRLCGELCLTHLGGPMLGAVTAD
                     GVRVWLRTLKPAAVEVRVTTGDGERVFGPVHSTPDSDLTAVVTVTGLKPATRYPYRVL
                     VDGQPITMPDHAAIVTAPDPAETGTMRIAFGSCFHRWGIGNAAQAQLIRSRNPVAMLL
                     LGDIAAQDRNNHLGLHRADYLMRDLQPPWQGLAAAIPLFAIWDDHDYFDNDLAGIPEG
                     FAEEDRQGVRQVFQQAWNNPAYGFGDTRGGL"
     gene            1110..1850
                     /locus_tag="PKW60_05955"
     CDS             1110..1850
                     /locus_tag="PKW60_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPJ99010.1"
                     /translation="MFLDDLARKIRAQREKRGLKQQDIANALNVSPQAVSKWERGENA
                     PDIAVLAPLARILGVSTDWLLSIHDPGTDEFEATVLVSSVRGAYKKSLDMAPRDFALW
                     ANGIFYQLTEVTLRHEGVPLKYLGDQYLCFFAGVRHQERALMTALEAKRLVSEDVKFA
                     LSVGPVYLGAVGHPDYVRPDIMGEAVNVAFLANEWADTHGKTGIAATASVLDGTEVSH
                     ARSKPHLVSFKGIQHKVRVYEVSEKSRK"
     gene            1982..3424
                     /gene="ahcY"
                     /locus_tag="PKW60_05960"
     CDS             1982..3424
                     /gene="ahcY"
                     /locus_tag="PKW60_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPJ99011.1"
                     /translation="MATVSETAAYPYKIADISLADWGRKEIDMAEKEMPGLMAIREKY
                     APQQPLKGYRVSGSLHMTIQTAVLIETLQALGADVRWASCNIFSTQDHAAAAVAERGT
                     PVFAWKGETLEEYWWCTEQALTWPDGKGPNLVVDDGGDATLLIHRGYEFEEAFAQTGK
                     LPEISRQNKEITIVDQLLHRIHGETPRKWHEYVEDWIGVSEETTTGVHRLYKMMKEGK
                     LLTRAMNVNDSVTKSKFDNLYGCRESLADGIKRATDVMVAGKVVVVAGYGDVGKGCAQ
                     AMRGLGARVLVTEIDPICALQACMEGYQVVTMEEAAPIGDIFVTTTGCCDVVRGEHFD
                     VMKDEAICCNIGHFDSEIQVSYLEDREDITELNIKPQVDKFIYPDGKAIILLARGRLV
                     NLGCATGHPSFVMSNSFSNQTLAIVAFATETASYEKGQVYTLPKTLDEEVARLHLGKL
                     GARLERLTPEQAEYLGVPVEGPYKPEHYRY"
     gene            3667..5211
                     /gene="sufB"
                     /locus_tag="PKW60_05965"
     CDS             3667..5211
                     /gene="sufB"
                     /locus_tag="PKW60_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPJ99012.1"
                     /translation="MPQEKKPITANANAAPENPGDSALTREYTGTRVERSNREIAELA
                     NRTYEHGWATDIEMELAPHGLDEDVIRLISGKKEEPEWLLEWRLKAYRHWKAMTEPRW
                     ANVRYPEIDYQAQRYYAAPKRKAQPLNSLDEVDQKLLQTFEKLGIPLEEQKRLSGVAV
                     DAVFDSVSVATTHQEILGRKGIIFSSFSEAVKNHPDLVRRYLGSVVPYTDNFFAALNS
                     AVFSDGSFCYIPKGVRCPMDLSTYFRINAIDTGQFERTLIIAEEGANVNYLEGCTAPR
                     RDENQLHAAVVELIALDNATINYSTVQNWFSGDEQGRGGIFNFVTKRGKCLGRNSRIS
                     WTQVETGSAITWKYPSCILQGDNSVGEFYSVALSTNHQQADTGTKMIHLGKNTSSTII
                     SKTISAAHGNNSYRGMVKVMPRAENARNYTQCDSLLIGDQCGAHTFPLIEVRNKTASV
                     EHEASTSKISEDQLFYCKQRGIGPEDAISMVINGFCKDVFDHLPMEFAVEATQLLSVS
                     LEGSVG"
     gene            5212..5979
                     /gene="sufC"
                     /locus_tag="PKW60_05970"
     CDS             5212..5979
                     /gene="sufC"
                     /locus_tag="PKW60_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPJ99013.1"
                     /translation="MLEISNLHAKVEDIQILKGVDLTVNPGEIHAIMGPNGSGKSTLA
                     QVLAGHEQYDVTQGEVTFLGQDLLEMPAEERARLGLFLAFQYPVEIPGVTNAYFLHAA
                     LNEIRKSHGLEELDAIDFRQRLEEKMEFVAIDPAFADRPVNEGFSGGEKKRNEILQMA
                     VMEPRLAILDETDSGLDIDALRIVADGVNKMRSPERSMILVTHYQRLLDYIVPDFVHV
                     MYQGRIVRSGGRELALELEEKGYEWVRDGVRTAQAAG"
     gene            6009..>7076
                     /locus_tag="PKW60_05975"
     CDS             6009..>7076
                     /locus_tag="PKW60_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPJ99014.1"
                     /translation="MVEVVEQLSDEEKLLEILARRDGSHASGWLRQFRDAGLRRVEAR
                     SFPTRKDEEWRFTDIRPIVRGAFEPAMRPPAEAPDPGLAGPHLFNAPGWTELVFVDGF
                     FAPRASSLANTSGLRILSLAESARAGDTFAQQHLDTLAGRSNNIFNAVNSACLTDGAV
                     VHVPRGYASAEPLHILYITTDKLEGKAAHPRNLIVLDETAELTLIESYVGLTGVPYFN
                     NAVTEIVLGGASVLKRYKVLHEGPQGYHLASTLVEQHADSVFRSFSAFLNGKIARNEL
                     KTRLAGEGANVSLAGLYLTETGQLIDNATSIEHVSPRCGSRIRYKGVLNGTSQAAFSG
                     AVYVHREAQKTDSNQLNSNLLL"
BASE COUNT         1517 a         2165 c         2167 g         1227 t
ORIGIN      
        1 agcccgccgc gcgtgtcgcc aaacccgtag gcggggttgt tccaggcctg ttgaaacacc
       61 tggcggacgc cttgtcggtc ttcctcggcg aatccttcgg gaatgcccgc cagatcattg
      121 tcgaaatagt catgatcatc ccaaatggca aacagaggga tcgcggcggc aagcccctgc
      181 cacggtggct gcaggtcacg catgaggtag tcggcacggt gcaggcccag atgattgttt
      241 cgatcctggg cagcgatatc gccgagcagg agcatggcaa cgggatttcg ggaccggatg
      301 agctgggcct gggcggcatt gccgatgccc catcggtgaa agcagcttcc aaaggcaatg
      361 cgcatcgtcc ccgtctccgc tgggtccggg gcagtgacga tcgccgcgtg atccggcatg
      421 gtgatgggct ggccgtccac cagcacgcgg taggggtagc gtgtggccgg tttcaggccc
      481 gtgacagtca ccacagcggt gagatccgaa tctggcgtgc tgtgaaccgg gccaaagact
      541 cgttccccgt caccggttgt cacccgcacc tcgaccgcgg ccggtttcag ggtgcgcaac
      601 cagacccgca cgccgtcagc cgtcacggcg cccagcatgg ggccgcccag atgggtgagg
      661 caaagctcgc cgcagaggcg ctggatttcc gggtcctcag tcatctccag aaacgccgcc
      721 cggggatctg ccgccagaat gccgtaggca gccgcataga accgctgcac gggttcgggg
      781 cgggtttcga acagattaaa tatggacagg ttggattcga ggtcgccttt cgatgtgccc
      841 tgaagcacct ccgtttcgcc ccaggccagg ggaatcgcca gcgccgcaag ggtcaggaac
      901 agagcgagta tcggtttcat ggaacttcct cgtcagttgc ggtgtcccgg gtgcctgcat
      961 cccagtctag cggatgagcg tcatatgaga aaagatgggg acgggcccgc ccgtgccaac
     1021 cggacgggga atgaggtcaa ccgatgcgtc attgccggcc ggcacatcaa ttaggtacgc
     1081 tatacggtgt ggctcgtgag gatggagcga tgtttttaga cgacctggcg cgcaagatcc
     1141 gtgcgcaacg cgagaaacgc ggcctgaagc aacaggatat cgcgaacgcg ttgaacgtga
     1201 gtccgcaggc cgtgtcgaaa tgggaacggg gcgaaaatgc cccggacatc gcggtgctgg
     1261 ctccgctggc ccggatcctg ggcgtgtcga ccgactggct cttgtcgatt cacgacccgg
     1321 gtacagacga attcgaggcg accgtgcttg tgtcaagcgt gcgcggcgcc tacaagaaat
     1381 cgctggacat ggcgccccgc gatttcgcgc tgtgggccaa cgggatcttc taccagctca
     1441 cggaggtgac cttgcggcat gaaggcgttc cgctcaagta cctcggcgac caatacctgt
     1501 gtttcttcgc cggggtgcgg caccaggagc gcgccttgat gactgcgctc gaagcgaaac
     1561 gcctggtctc cgaagatgtg aaattcgccc tgagcgtcgg gccggtctac ctcggcgcgg
     1621 tggggcatcc ggactacgtc cggcccgaca tcatgggcga agcggtcaac gtcgcctttc
     1681 tggccaacga gtgggccgac acccacggca agactggaat cgccgccacg gcctccgtgt
     1741 tagacggaac ggaagtcagc catgcccgga gcaaaccgca tctggtcagc ttcaagggca
     1801 tacagcacaa agtccgggta tatgaggtga gtgaaaagag ccggaaatag gcgcggcctc
     1861 ggcctggctt cttcgaaaat ctcaaaaaca ccagaaccag cgcgtatgcg cgttatttca
     1921 tcgacaacca gcgcggaccg tgcgcgacac gccgttccgc ccagggagga gcatcactat
     1981 catggcgacg gtatcggaga cggcggcata tccttacaaa atcgcggaca tctcgctggc
     2041 ggactgggga cgcaaagaga tcgacatggc cgaaaaggaa atgcccggct tgatggcgat
     2101 ccgcgagaaa tatgcgccgc aacagcccct taaagggtat cgcgtctcgg gttcgctgca
     2161 catgaccatc cagaccgcgg tgctgatcga gacgctgcag gcgctcggag ccgatgtccg
     2221 ctgggcaagc tgcaacatct tttcgacaca ggaccacgcg gccgcggctg tcgcagagcg
     2281 cggcacgcct gtatttgcgt ggaaaggcga gaccctcgaa gaatactggt ggtgcacgga
     2341 gcaggccctg acatggccgg acggcaaagg cccgaatctc gtcgtggatg atggcggcga
     2401 cgcgaccctg ctgatccatc gaggctacga attcgaggaa gccttcgccc agaccggcaa
     2461 actcccggag atctcgcggc agaacaagga aatcaccatt gttgaccagc tgctccaccg
     2521 gattcacggg gaaacccccc gcaaatggca tgagtacgtg gaagactgga tcggcgtctc
     2581 cgaggaaacc accaccgggg tgcaccgcct ctacaaaatg atgaaggagg gtaagctgct
     2641 cacccgggcg atgaacgtca acgattcggt cacgaaatcg aagtttgaca acctctacgg
     2701 ctgccgggaa tcgctcgcgg acggcatcaa gcgcgccacg gacgtcatgg tggcgggcaa
     2761 ggtcgtggtc gtggccggct acggcgacgt gggcaagggc tgcgcgcagg ccatgcgggg
     2821 tctgggcgcc cgggtgctgg tcacggagat cgatcccatc tgcgcgctcc aggcctgcat
     2881 ggaaggctac caggtcgtca ccatggaaga ggccgcgccc atcggcgaca tcttcgtcac
     2941 gaccacaggc tgctgcgacg tggtccgcgg cgagcatttc gatgtgatga aggacgaggc
     3001 catctgctgc aacatcggcc acttcgactc ggagatccag gtgtcgtatc tcgaggaccg
     3061 cgaagacatc acggaactca atatcaaacc ccaggtcgac aaattcatct accccgacgg
     3121 caaggcgatc attctcctgg cgcgggggcg cctggtaaat ctgggttgcg ccaccggaca
     3181 cccgtccttc gtgatgtcca actcattctc aaatcagacc ctcgcgatcg tggcgtttgc
     3241 caccgagacc gccagctacg agaagggcca ggtctatacc ctccccaaaa ccctcgacga
     3301 agaagtcgcc cgcctgcatc tcggcaagct tggcgcccgg ctcgagcggc taacgcccga
     3361 acaggcggag tatctcggcg tgcccgtcga agggccgtac aaacccgagc actaccggta
     3421 ttagcgccac cgcaaacggg cgcctggcgg cagcgggagt gcgcgccgcg tcccggtgaa
     3481 agatcgctgc gccaccgcgg atgaatgccg cacatttccg tgttcactta aacgagtcgc
     3541 ggctgccggg gcggtgcctc ccgcaaccgg cctcctgtca ggaataccgc cccggccggg
     3601 aatgtttgcc tcagggtatg ggggcaacgc tatccgcgca accgccccgg agtggaataa
     3661 acccgtatgc cacaagaaaa gaaacccata acggcgaacg cgaacgcggc gcctgaaaac
     3721 cccggcgact cggctctgac ccgcgaatat accggcacgc gcgtggaacg cagcaaccgc
     3781 gagatcgctg aactggccaa ccggacttac gagcacggat gggcgaccga catcgagatg
     3841 gaattggcgc cccacggcct cgacgaggat gtcatccgcc tgatctccgg caaaaaagag
     3901 gagcctgagt ggctcctgga atggcgcctg aaagcgtacc gccactggaa agcgatgacg
     3961 gaaccccggt gggccaacgt gcgctacccc gaaattgact accaggccca gcgctactac
     4021 gcggcgccga aacgcaaggc ccagccgctg aacagcctcg atgaggtcga tcaaaaactc
     4081 ctgcagacct ttgaaaaact gggcatcccg ctcgaagaac aaaagcggtt atccggcgtg
     4141 gccgtggacg cggtattcga cagcgtatcg gtggctacga cccaccagga gattctgggc
     4201 agaaagggca tcattttcag ctcgttttcc gaggccgtga aaaatcatcc cgacctcgtt
     4261 cgacgctacc tgggaagcgt ggtgccgtat accgacaact tcttcgcggc gctgaattct
     4321 gcggttttca gcgatggatc gttctgctat atcccgaagg gcgtccgctg cccgatggac
     4381 ctgtcgacgt atttccgcat caacgcgatc gacacgggcc agttcgagcg cacgctgatc
     4441 atcgccgaag agggcgccaa cgtgaattac ctcgaaggat gcaccgcccc ccggcgcgac
     4501 gaaaaccagc tccatgcggc agtcgtggaa ctgatcgccc tcgacaacgc caccatcaat
     4561 tattcaacgg ttcagaattg gttcagcggc gacgaacagg gccgtggcgg cattttcaac
     4621 ttcgtgacca agcggggcaa atgcctcggc cgcaattccc gcatctcgtg gacccaggtc
     4681 gagacgggtt ccgccatcac ctggaaatac cccagctgca ttcttcaggg cgacaattca
     4741 gtgggcgaat tctattctgt cgccctgagc accaaccacc agcaggcgga tacgggcacc
     4801 aagatgatcc atctgggcaa aaacacctcc agcacgatca tctcgaaaac catctcggcc
     4861 gcccacggca acaacagtta ccgcggcatg gtgaaggtga tgccccgggc tgagaatgcg
     4921 cggaattata cccagtgcga ttcgcttctc atcggggatc agtgcggtgc gcataccttc
     4981 ccgcttatcg aagtgcgcaa caaaacagcc agcgtggagc acgaggcgtc cacatccaaa
     5041 atcagcgaag atcagttgtt ttactgcaaa cagcgcggga tcggtcccga agacgccatt
     5101 tcgatggtca tcaacggatt ctgtaaagac gtgttcgacc atctgccgat ggaattcgcg
     5161 gtagaggcca ctcagttgtt gagcgtgagc ctcgaaggga gtgtaggtta aatgctcgag
     5221 atcagtaatt tgcacgcgaa agtcgaggac atccagatcc tcaagggcgt cgatctgacg
     5281 gtcaacccgg gcgagatcca tgccattatg ggccccaacg gctcaggcaa gagcaccctg
     5341 gcccaggtcc tagcgggcca cgagcaatac gacgtcacgc agggcgaagt cactttcctg
     5401 gggcaggatc tgctcgagat gcccgcggag gaacgtgcgc gcctgggcct ttttctggcg
     5461 tttcagtatc ccgtcgagat ccccggcgtg accaacgcgt acttcctgca cgccgcgctc
     5521 aacgaaatcc gcaagagcca cggcttggaa gaactcgacg ccatcgattt caggcaacgg
     5581 cttgaagaaa agatggagtt cgtggcgatc gaccccgcgt ttgcggaccg ccccgtgaac
     5641 gaaggatttt cgggtggcga aaagaagcgc aacgaaatcc tccagatggc cgtcatggaa
     5701 ccccggctgg ccattctcga cgaaaccgat tcgggcctcg acattgacgc gctgcgcatc
     5761 gtcgccgacg gcgtcaacaa aatgcgcagc cccgaacgct cgatgatcct cgtaacgcat
     5821 taccagcgcc tgttggacta tatcgtcccg gatttcgtcc acgtgatgta ccagggccgg
     5881 atcgtccgct cgggcggcag ggaactggct ttggaactcg aggaaaaagg ttacgaatgg
     5941 gtgcgggacg gcgtgcgcac cgctcaggcc gccggttgaa ccaaaccgca cgacgctgga
     6001 gtatggcgat ggttgaagtc gtagaacagc tttccgacga agaaaaactc ctcgagatcc
     6061 ttgcccgaag ggacgggagc cacgcctccg gctggttgcg ccagttccgc gatgcaggcc
     6121 tccggcgcgt tgaagcccgg tcgtttccga cgcgcaagga cgaggaatgg cggttcacgg
     6181 atatccggcc gattgtgcgg ggggcctttg aacccgcgat gcgcccccct gctgaagcgc
     6241 ccgaccccgg tctggcgggg ccgcatctgt tcaatgcgcc cggctggacc gaactggtct
     6301 ttgtagacgg gtttttcgct cccagagcct catctctcgc gaacacgagc gggttgcgta
     6361 tcctcagcct ggccgagtcc gcccgagccg gcgatacgtt tgctcaacag cacctggata
     6421 ccctcgcggg gcgctcgaac aacatcttta acgcggtgaa ctcggcctgc ctcacagacg
     6481 gcgccgtggt gcatgtgccg cgcgggtacg catccgccga gccgctgcac atcctctaca
     6541 tcacgacgga caaactggag ggcaaggcgg cgcatccgcg aaacctgatt gtcctcgatg
     6601 aaaccgccga gctgacactt attgaaagct acgtgggctt gacgggggtg ccgtatttta
     6661 acaatgccgt caccgagatc gtcctgggcg gcgcgtcggt attgaagcgg tacaaagtgt
     6721 tgcatgaagg cccgcaaggc tatcatctcg cgtccacgct tgttgaacag catgcggaca
     6781 gcgtgtttcg ctcgttctcg gcctttctga acggcaagat cgcccggaac gagctgaaaa
     6841 cccggctggc gggcgaaggc gcaaacgtgt ctcttgccgg cctctatctg accgaaaccg
     6901 gccagctgat cgacaatgcc accagcatcg aacacgtcag cccccgttgc gggagccgca
     6961 tccggtacaa gggagtcctc aacggcacga gccaggcggc attcagtggc gcggtctatg
     7021 tccatcgcga agcgcagaaa accgactcca atcagctcaa cagcaacttg ctcctg
//
LOCUS       DAOPBQ010000138         7055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9565_length_7055_cov_4.775857, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000138 DAOPBQ010000000
VERSION     DAOPBQ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9565_length_7055_cov_4.775857"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="PKW60_05980"
     CDS             complement(<1..1337)
                     /locus_tag="PKW60_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99015.1"
                     /translation="MRKYAVGLSVLVLLWPSVPASAWGPQSQIAIVTTAGRMLSKEGA
                     VPLSKMEKEIQQGASLTSGELALLMPNADLQPARSIFTEMLLLQTIRESGVDPYFAFR
                     LGALGRVVAGVTAPMQTADPVHRKMYYDDVDQRISDTGLTLQARQMVDSMEYFTRINR
                     ELEERQNMLLTDYRSGLGFDGVAKATYGQDLSRSINAVGDVWYTVLQRRGVSAGVSLD
                     QKRNYALRAMAFYVDRANIGEINAAHKRLEALGVYTSDVLKEIGDMMYEAGMRERAVE
                     EYLAVLKKEPERKDVVRRIADYYIDLADEELERDRLETARDYYARALEVDPLHPLAES
                     KRLTAEKLIRERDARLAQARDAIAKARELEENAEVQASTKRYAEAIAQLRQALDFYRG
                     VTNEFVVEKRAAQAGVNNVTSRMRDLRIRLQESASGLSGAGSSVDTVNLAEREA"
     gene            complement(1356..2939)
                     /locus_tag="PKW60_05985"
     CDS             complement(1356..2939)
                     /locus_tag="PKW60_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ99016.1"
                     /translation="MASSMMNCPYCGKLTDPRLDNCVHCGGFLRKKAAPGQRRSSGEK
                     ETCPTCGALVQAGDIICVACGTNLLTGQRITEEQPAPAKAKKRAFTMPSVNLKWVGFA
                     TLGFLVVVILLFLLALFLRRDRIGAAIQEYNKGNATQAVAALNDYIQSVPDSVEARQA
                     LAWIYWENKEYAFAAEQLASVVRLDPGNREAHRGLALGWARAGGEDGRRRARAALEAM
                     VRQFPGDSEAWYLLGLIRGVDGDVEGQVAAMREASAGSGASGLAAKRLTAVGIAMQGH
                     TESARQTIQKVLEEAPNDGEVLAAAGILASEESDWIAATEYLKAAVNEGTALEGGALT
                     ELGILLISRREFAEARGFLERALARDDNNHRARLFLAVCQLEQGLPEAALQQLEPLLK
                     TTGPLAAEAAVEAARAHLALNDPTRATQSLQQAIQLGGSDAALYTLQGRAYARTGEAV
                     AAREAFRKALLADSAYAPAHLEFGLFCLERGESTEAVRMLERYLELIDPACKDARIEE
                     VRTLVAQLKGALDAQGNAT"
     gene            complement(3304..6681)
                     /locus_tag="PKW60_05990"
     CDS             complement(3304..6681)
                     /locus_tag="PKW60_05990"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HPJ99017.1"
                     /translation="MRFACALVLSLLLPGAYAVEMPDYLLLVVESPPPDGIIARDLEW
                     AGFLKDAPVSVEPGALLEQEDGLRPVPLQYDAHDNGTGRLLLLLPGDLPSGARIRLYP
                     AGGPEHTPPSGARVSATSEDGVITVNNGIIEVVHNPAVMAGLPSKVTFLETGRVFEDY
                     MLNDRIYDPELGTFALRFDTEPEIRLLSSGPIEAVVEVRARYVQDATPAPGDARAVYR
                     FRYRAGSPAIAIDAVCTQDELHSWPELHLAEWHFPSRPLPQWAAGAPFQQGTFQEDAQ
                     TFQGVSWGGLTDGTHLFGLFGGPIILYSGATNHGSYVHGPWVTWDSLETELRTTAWLD
                     AGAEAVAALSSVATAPARPVRTRISTAPLEELREALAERLDAADPDDRRAQHAAWSLS
                     WYSRPAVWEASLEETEERLQSLSRIMEGPGSREIGEQQTQQLCLYDEALGVIAFDPAD
                     GRALPSLFSLAGRREFLTDDATPLWTMAVEDSKGRESVWNSLDTFFSCEAADPDDPAA
                     RHAQVRLIWTGHGEAEGFTATVDMALETLGLTMALKIDNDTDTITVNRVDFPVLSLGP
                     VGDSADDDCFLAPTVSGTLWPAPLINGLSVRAEYPGGWGCMQFGAHYDPDAGLYIAVH
                     DPVASAKRFRAFRHGTRNALDVRIEWPVPDASLPGNDFEMPGPAVLAAFQGDWFDAAG
                     RYRAWAEREAAWWPAREHWGRPDIPAWLLDICVWGRPSGAASSAVPQTLAFAEYLGVP
                     TAVHWYVWHEIPFDNDYPHYFPAKEGFAEGVAALHDAGVRVMPYINGRLWDSDTDDFQ
                     TIALPAATKKRNGEPYIEHYGEDPEDLVPMCPTQKVWQDKVQEIVLRLLGPEFNVDGV
                     YIDQVAAAPPAECYDRSHGHPLGGGAWWTTLGYWPMLTELNAAIDREFPDKMLTSECT
                     AEPFTHLFNGYLTWHFQYNNAVPAFAAVYGGKIVLFGRSDGGEDLQAHWARTGQALVW
                     GEQLGWYGTDYVERLPRAAEYLRRCARIRSRLLPYLARGRMLRPPAPGGELPQVTANW
                     AWGGGDWPVTTPALQTGAWRAEDGFVAVIFANTIERPLSFSWVIPSAEYGLSEESRPC
                     SRITEDGVVAAEPVTPGTELTLTLEPLAVTALVIPGG"
     gene            complement(6750..>7055)
                     /locus_tag="PKW60_05995"
     CDS             complement(6750..>7055)
                     /locus_tag="PKW60_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPJ99018.1"
                     /translation="LNECRALVGADSDIPAAMLTRATEITDSNAAFFDAANAFAGCIA
                     GIHEVLVRQGLMASARCLNPNETLSPGQRDEIDRVYRAYPHLNDDAFVEQHLDEWLA"
BASE COUNT         1264 a         2301 c         2207 g         1283 t
ORIGIN      
        1 gcctctcttt cggccaggtt gaccgtgtcg acgctgctgc ccgcgccact caacccggag
       61 gcgctctcct ggagccggat gcgcaaatcg cgcattcgtg aggtcacgtt gttaacgccg
      121 gcctgcgcgg cccgtttctc gacgacgaat tcgttggtga cgccgcggta aaaatcgagg
      181 gcctggcgca actgcgcgat ggcttccgca taccgtttgg tggaggcctg cacttcggcg
      241 ttttcttcga gttcccgcgc cttggcaatg gcgtcgcggg cctgggccag gcgggcgtcg
      301 cgctcccgga tgagtttttc ggcggtcagg cgcttcgatt cggcgagcgg atggagggga
      361 tccacctcga gcgcgcgcgc ataataatcc cgcgctgttt cgaggcgatc gcgctccagt
      421 tcctcgtcgg ccaggtcaat gtaataatcc gctatgcgcc gcacgacatc tttgcgttcc
      481 ggctctttct tcaggacggc cagatattct tcaacggcgc gttctcgcat accggcctcg
      541 tacatcatat cgccgatttc tttcaggacg tcgctggtat agacgccgag cgcctcgagc
      601 cgtttgtgcg cggcgttgat ctcaccaatg ttggcgcgat cgacgtagaa ggccatggcg
      661 cggagggcgt aattacgctt ctggtcaagc gatacgcccg cggaaacgcc gcggcgctgc
      721 aggacggtat accagacgtc gcccaccgca ttgatggaac ggctgaggtc ctgcccgtag
      781 gtggctttag ccacgccatc gaagcccagc ccggatcggt agtcggtgag gagcatgttc
      841 tggcgttctt cgagttcacg gttgatccgg gtaaagtact ccatgctgtc gaccatctgg
      901 cgcgcctgga gagtcaaacc ggtatcggag atgcgctggt cgacgtcgtc gtaatacatt
      961 tttcgatgca cggggtcggc cgtctgcatg ggggcggtca cgccggcaac cacgcgcccc
     1021 agcgcgccca gccggaacgc aaaatagggg tccacgcccg attcgcgtat cgtctgaagc
     1081 agcagcatct cggtgaaaat ggagcgggcg ggttgcaaat ccgcattggg catcagcagg
     1141 gccagttcgc cagaggtaag cgacgcgccc tgctggatct ccttctccat ttttgacaga
     1201 ggcaccgcgc cttctttgct cagcatgcgc ccggccgtgg tgacaatggc gatttgcgat
     1261 tgcggccccc acgcgctcgc ggggacactg ggccacagca gcaccagcac cgacaaacct
     1321 accgcatatt tccgcatcgg cagtttcctc gcggtttatg tggcgttgcc ctgggcgtca
     1381 agcgctccct tgagctgcgc cacgagcgtc cgcacttctt caatacgagc atctttacag
     1441 gccgggtcta tgagttccag atagcgttcc agcatgcgaa ccgcttctgt cgattcgccg
     1501 cgttcaagac aaaacagccc gaattcgagg tgggcggggg catacgccga atcggccaga
     1561 agcgctttcc ggaaggcttc ccgcgcggcc acggcttcac cggtgcgcgc gtatgcgcgc
     1621 ccttgcagcg tatacagcgc cgcgtccgag ccgcccagct ggatggcctg ctgcaggctc
     1681 tgggtggccc gcgtgggatc gttcaaggcc agatgcgccc gggcggcttc gaccgctgcc
     1741 tcggcggcca acggtcccgt cgtcttcaac agaggctcca actgctgcaa tgccgcctca
     1801 ggcagccctt gttccagctg gcagaccgcg aggaacagcc gcgcccggtg gttgttgtcg
     1861 tcacgggcaa gcgcgcgctc gagaaagcct cgggcttcgg cgaactcccg gcgggaaatg
     1921 agcaggatac ccagctcggt cagggcgccg ccttccagtg ccgtgccctc gttgacagcg
     1981 gctttcaaat attccgtcgc ggcaatccag tcgctctctt ccgatgccag aatgccggcc
     2041 gccgcgagga cttcgccatc attgggcgcc tcctcgagta ctttctgaat ggtttgtctg
     2101 gccgattccg tatggccttg catggcgatg cccacggcgg tcaggcgctt cgcggcaagt
     2161 cccgaggcgc cagaaccggc cgacgcttcg cgcatcgccg cgacctgccc ttccacatcg
     2221 ccgtccaccc cgcgaatcaa tccaagcagg taccaggctt ccgagtcgcc ggggaactgc
     2281 cgaaccatcg cttcgagtgc ggcgcgggca cggcggcggc cgtcttcacc gccggctcgg
     2341 gcccagccca gggccagacc ccgatgggcc tcgcggtttc ccgggtccag acgcaccacg
     2401 ctggccaatt gctccgctgc gaatgcgtat tccttgttct cccagtaaat ccaggccagt
     2461 gcctggcgcg cctccaccga gtcgggcacg gattgaatgt agtcgttcag ggcggccacg
     2521 gcctgggtcg cattgccttt gttgtattcc tggatggcgg ctccgatgcg atcgcgccgg
     2581 agaaacagcg ccagcagaaa cagcagaatg acaaccacca aaaatcccag cgtggcaaat
     2641 cccacccact tcaggttgac ggacggcatg gtgaacgccc gctttttggc ctttgccggt
     2701 gcgggctgtt cctccgtgat gcgctgcccg gtcagcaggt tcgtgccgca tgcgacgcaa
     2761 atgatgtcgc ccgcctgcac cagcgcgccg caggtggggc aggtctcctt ttccccgctg
     2821 ctgcgccgct gccccggcgc cgccttcttg cgcaggaatc cgccgcaatg cacgcagttg
     2881 tccagccggg gatccgtcag cttgccgcag tacgggcaat tcatcattga cgatgccacg
     2941 ttcacttctc ctcgtgcccg ccggccagcc ggcttgtaga acaccgcctg ttacgcgccg
     3001 tcgtacggca acggcgctgc acaagattta cagttccggg cctcaataaa attccgcgtg
     3061 ccgcagtgga cgcacttctt gaaaaacaac ggcggcacac tcaccagcgt acagaccgca
     3121 atcaccgcca gcatgatcgc cacataggct ccagcggcca tctgtccggc aacctccctg
     3181 ccgcgtagtg tcctgccccg tctgacagcg tcctccctgt tgcatcacgc tcattctaga
     3241 cgccgggtta tctcttgtca acggaagtcc ttacagcaaa cgtccttact tttggcctgg
     3301 gactcatcca cccgggatga ccagggcggt taccgccagc ggttccaggg tcagggtgag
     3361 ttcggttccg ggggtcacgg gctcagccgc cacgaccccg tcttcggtaa tacgggagca
     3421 gggccgcgat tcttcggaca agccgtattc ggcgctgggg ataacccacg agaacgacag
     3481 cggacgctca atggtattgg cgaagatcac agccacgaag ccgtcttcgg cgcgccaggc
     3541 gccggtttgc agggccggcg tggtcaccgg ccagtcgccg ccgccccagg cccagttggc
     3601 cgttacctgg ggaagctcgc cgccgggggc gggcggccgc agcatacggc cccgcgcaag
     3661 atacggcagg aggcggctgc ggatcctcgc acaccgccgg aggtattcgg cggcgcgagg
     3721 caggcgctcg acgtaatcgg tgccgtacca tcccagctgt tcaccccaga ccagagcctg
     3781 accagtccgc gcccagtgcg cctggagatc ctcgcctcca tccgaacggc caaacaagac
     3841 aattttgccg ccatatacgg ccgcaaacgc gggcacggcg ttgttgtact gaaaatgcca
     3901 ggtcagatac ccgttgaaga gatgggtgaa cggttccgcg gtgcattcac tggtaagcat
     3961 tttgtcggga aattcccggt cgatggcggc attgagttcg gtgagcatcg gccagtatcc
     4021 gagcgtggtc caccaggcgc ctcctccgag tggatggccg tggctgcggt cgtaacattc
     4081 ggcggggggc gccgcggcca cctgatcgat gtagacgccg tccacattga actcgggtcc
     4141 gaggaggcgc aagacgatct cctggacctt gtcctgccag accttctggg tcgggcacat
     4201 gggaacgaga tcttccggat cctcgccgta atgctcaatg tatggctcgc cgttgcgttt
     4261 ttttgtggca gcgggcaggg ctatggtctg gaaatcgtcg gtgtcggaat cccagagccg
     4321 gccgttgatg tagggcatca cccgtacacc ggcatcgtgc agcgcggcaa cgccttccgc
     4381 gaacccctct ttcgcgggga aatagtgggg ataatcgttg tcgaagggaa tttcatgcca
     4441 tacataccag tgcactgccg tcggcacgcc caggtattcg gcaaaagcga gcgtttgagg
     4501 gaccgcggag ctggcggcgc cgctgggccg gccccagacg cagatgtcca ggagccacgc
     4561 gggaatgtcg gggcggcccc agtgctcccg cgccggccac caggccgcct cgcgctcggc
     4621 ccaggcgcgg tagcgccctg ccgcatcaaa ccagtcgccc tggaacgccg cgagcacggc
     4681 cggccccggc atctcaaaat cattgcccgg caacgacgcg tccggcaccg gccattcaat
     4741 gcggacatcg agggcgtttc gggtcccgtg gcgaaatgcg cggaagcgct tggccgaggc
     4801 gaccgggtcg tgcacggcaa tgtaaagccc ggcgtccgga tcgtaatgcg cgccaaactg
     4861 catgcagccc cagccgcccg ggtactcggc cctcacggag agcccgttga tcagcggcgc
     4921 aggccacaac gtccccgaga ccgtcggcgc gagaaaacaa tcgtcatccg ccgagtctcc
     4981 aacaggaccc agcgagagca ccggaaaatc gacgcgattc accgtgatcg tgtccgtatc
     5041 gttgtcgatc ttgagggcca tggtgaggcc cagcgtttcc agggccatgt cgaccgtcgc
     5101 ggtaaagcct tcagcctctc catgccccgt ccaaatcagg cggacctgtg cgtgacgggc
     5161 tgcgggatcg tccggatcgg ccgcttcgca cgagaaaaag gtatccagac tgttccagac
     5221 gctttcccgg ccttttgaat cctccacagc catggtccac aggggcgttg cgtcgtccgt
     5281 caggaactcg cgccggcctg ccaggctgaa gaggctcggc aaggcgcgcc catccgccgg
     5341 atcaaatgcg atgaccccca gcgcctcgtc gtacaggcac agctgctggg tctgctgctc
     5401 gccaatctcg cgcgagccgg gcccctccat gatgcgcgag agcgattgaa gccgttcttc
     5461 agtttcttca agcgaggcct cccagaccgc agggcgggag taccacgaga ggctccacgc
     5521 cgcatgctgc gcgcgccgat catccggatc agccgcatcg agccgttcag cgagtgcctc
     5581 gcggagctct tccagcggag ccgtgctgat ccgtgttcga acaggccggg cgggcgcggt
     5641 cgctaccgat gaaagcgccg ccacggcctc agcgcccgca tcgagccagg ccgtcgtgcg
     5701 cagctctgtc tcgaggctgt cccaggtgac ccagggcccg tggacgtagc tgccgtgatt
     5761 tgtcgctcct gaatacagaa tgatggggcc gccaaacagt ccgaacaggt gcgttccatc
     5821 ggtcaagccg ccccacgaga cgccctgaaa ggtctgggca tcttcttgaa aggtcccctg
     5881 ttggaatggt gctccggcag cccattgcgg cagcggccgc gacgggaaat gccattcggc
     5941 caaatgcagt tccggccagg aatgcaattc atcctgagta cagacggcat cgatcgcgat
     6001 cgccggcgat ccggcgcggt accggaaccg gtacaccgcg cgcgcatcgc cgggggcagg
     6061 ggtggcatcc tggacatacc tggcccggac ctcgacgacg gcctcgatgg ggccggagga
     6121 aaggaggcgg atttccggtt cagtgtcgaa gcggagcgcg aacgtcccca gttccggatc
     6181 gtagatccgg tcgttgagca tatagtcctc aaacacccgc cctgtttcga ggaacgtgac
     6241 ttttgaggga agtccagcca taaccgcagg gttgtggacg acctcaataa tgccgttatt
     6301 gaccgtgatg acgccgtctt cgctggtggc ggaaacccga gccccggacg gcggcgtgtg
     6361 ttccgggcca ccggcgggat acaggcggat acgtgcaccg gaagggagat ctcccggcag
     6421 caggagcagc aggcggccgg ttccattgtc gtgtgcatcg tactgaagcg gtaccggccg
     6481 cagtccgtcc tcttgttcga gcaacgcacc cggttcaaca gaaacgggcg catccttcag
     6541 gaaacccgcc cactccaggt cgcgcgcgat gatcccgtcg ggcggcggcg attcaacgac
     6601 cagaagaagg taatccggca tttccacggc gtaggcgccc ggcagcagga gagagaggac
     6661 aagagcgcag gcgaatctca tggcgcgaac tccttccata aacgcccgta cgggcgcggc
     6721 gccaacggat aaacgcgcgg cgccggcgat tacgccagcc attcgtcgag atgctgctcg
     6781 acaaaggcat cgtcgttcag gtgaggatag gcacggtaga cacggtcgat ctcgtcgcgt
     6841 tgccctggcg aaagggtctc gttcgggttg aggcaccggg cggaagccat gagtccctgc
     6901 cgcaccagca cttcatgaat gcccgcgata catcccgcaa acgcattggc cgcatcaaaa
     6961 aacgccgcgt tgctgtcggt gatttcggtt gcgcgggtca gcatcgctgc cggaatgtcc
     7021 gaatccgccc cgacaagcgc ccggcactcg ttcag
//
LOCUS       DAOPBQ010000139         7033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9601_length_7033_cov_3.678131, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000139 DAOPBQ010000000
VERSION     DAOPBQ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9601_length_7033_cov_3.678131"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PKW60_06000"
     CDS             <1..731
                     /locus_tag="PKW60_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPJ99019.1"
                     /translation="GAKVVMSDRQPEKAEMAAQRIMAAGGTAMGVQMDAFERSTIEAC
                     RDMACDAYGSVDILVNAVGGNMAAATTSAEQSFFQLMPDALSQVFNLNMLAGTIVPCQ
                     VFGERMKDNPEGGSIINISSMSALTPLTRIPGYSAAKAAVNNFTQWLAVHFAQEYTAK
                     LRVNAVAPGFFLTEQNRFLMTDEKTGELTPRGQKVIDHTPMGEFGRPDDLIGAAIWLA
                     SSASRFVTGIVVPVDGGFSAYSGV"
     gene            781..1674
                     /locus_tag="PKW60_06005"
     CDS             781..1674
                     /locus_tag="PKW60_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPJ99020.1"
                     /translation="MIDPVASLKAHQPNHKFLIAIDSDGCAFDTMEIKHKECFIPNIV
                     KYWDLQPVSKYARAAAEFVNLYSKWRGINRFPALLMVFDLLDNWDKVQERDFRCPNVA
                     SLREWVVKESKLGNPVLEAWCKAHPNNPVMERALEWSKAVNRTVADIVQGGLPPFPYV
                     RECLEKAHARADMLVCSQTPTEALTREWQEQGVDAYVFAIAGQEMGKKSEHIGFACQG
                     RYEQTKILMIGDAPSDMQAARANNALFFPINPGDEDKSWKRLYEEGLDRFFAGEFAGE
                     YEASLIAEFDTYLPEVPPWKK"
     gene            1778..2917
                     /gene="pdxB"
                     /locus_tag="PKW60_06010"
     CDS             1778..2917
                     /gene="pdxB"
                     /locus_tag="PKW60_06010"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF001309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HPJ99021.1"
                     /translation="MRIVVDENMPYGFEAFSTLGDVISMPGRSIDPAAAANADILAIR
                     SITTVNAALLEGSRVRFVGTATIGEDHVDKAYLEARGIGFSSAPGCNANSVGQYVTAA
                     LLELAEAHAFRLRGTTLGIVGVGNVGSRVLKKASAMGLSCVLNDPPLERKTGDSAYRP
                     LDEILDCDIVTMHVPLTREGPDATYHLVDEAFVRRMKPGSILINTARGAVADGAALKR
                     ALADGHLKACVLDVWENEPTVDLELLEQTFIGTPHIAGYSFDGKVNGTRQIYEAACAF
                     LGVPPAWDPSPLLPAPECPEVHVNGAAEEPEGVLRDAVRRVYDIRNDDKAMRGLLHAP
                     ETEHGAFFDRLRKEYPRRREFFNTRAIITPENSDLAAVFSGVGFQ"
     gene            2930..4207
                     /locus_tag="PKW60_06015"
     CDS             2930..4207
                     /locus_tag="PKW60_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPJ99022.1"
                     /translation="MIELIKEHEALTNGEIQAFVSEAMASLDADGKRVLFIIPDQTRS
                     MPMPVVFRALHTALSRRVKKMDFLVALGTHPPLTETMLNTLLGVTPEERAGVFAGVGV
                     FNHAWDQPSQLARIGTISEEEIYAISGGLMRQHVGVTLNKMILDYDLLCVVGPVFPHE
                     VVGFSGGNKYFFPGIAGPDIINLFHWLGALISNPVINGTMHTPVREVVNRAAACIPVE
                     KWCLALNVMGKDCHGLFFGTPEAAWEAAAQMGTRTHIVYKDKPFHSVLALAPEMYDEL
                     WVAGKCMYKLEPVVADGGELIIYGKHIKEVSVTHGHYIRKIGYHTRDYFLSQMEKFTD
                     IPGGILAHSTHVRGIGEMLDGVEKPRIQVTLATSIPKEECEAINLGYRDPGSIDPDDW
                     RGREDEGVLLVPQAGEVLYRLREGNPRPKAFTP"
     gene            complement(4218..5438)
                     /locus_tag="PKW60_06020"
     CDS             complement(4218..5438)
                     /locus_tag="PKW60_06020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HPJ99023.1"
                     /translation="MLLAVLVVVYALAVAAKIAREPQHFQLTFRTCYAAAQAFSQGLD
                     PYDVPNLQQQDPRVQGAYGYPPITLYFFLPFAALPFQAAYMAFLAIKILCLAGLVWLW
                     HRYFLKRPLDGLFLLICLLGFNFALYWDLASGNVGLIEQFLLWMGLYAYLRGRLKTFA
                     VLVALSASFKLALILFLALLFIRRGRRRHYALFLGIGVYAAMLAFSSVFYSEMTIIFL
                     QNLQRSGLGLYGGGPLRAAPSSGAWRLLNELSATACGRLGTMMAAEPIAVAGYLVLVA
                     FVILMTWAAVRRYLHVFREDGYLIVIMLFCVAYLLVLPRLVPCSYILALPAAYCIALK
                     IRQVPALLALTGAAAMSTSAVQPPGFNLLKEVLPPYYPLLLTFILWAVGICFCMRAEA
                     RPKPRAEHAQPSEG"
     gene            5697..6983
                     /locus_tag="PKW60_06025"
     CDS             5697..6983
                     /locus_tag="PKW60_06025"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HPJ99024.1"
                     /translation="MPKNVIIAQSGGPSPVINSSLRGVIETCKMFPGTFGTLYGGWHG
                     IEGVLKEELLDLSAQDEEEVALLRYTPAAGSIGTCRYKLKSNQNEDFERVIDVMKAHD
                     IGFFFYNGGNDSMDTAHKIAQLAHERGLDLVAVGVPKTIDNDVGDSEFQLIDHTPGYG
                     SVARYWMSVVQNANEENCGSCPADPVLVLQAMGRKIGFIPAAARLADPKRQMPLQIYM
                     AESKVTVEELCDNVNDQLKRDGRCIIVVSEGFEVGELGETKDSFGHTQFSASKTTVAQ
                     QLVTILNEHGLPVPGKARGQVMGTDQRMTALYASIPDLDEAYKVGQKAALIAESGENG
                     WMSTILREPGPIYNVRYDKVPLEKVANSERAFPKAWLAPSKYDVTDEFIRYAQPLVGE
                     DWASVPVVNGRQRFTRFKPVFAEKMLPEYTLQALRK"
BASE COUNT         1561 a         2048 c         2159 g         1265 t
ORIGIN      
        1 ccggagcgaa agtagtcatg agcgaccggc agcctgagaa ggccgaaatg gccgcgcagc
       61 gcattatggc agccgggggt acggctatgg gcgtccagat ggacgcgttt gaacgctcca
      121 cgatcgaggc atgccgcgat atggcctgcg atgcctacgg atccgtggat attctggtga
      181 atgccgtggg cggcaacatg gcggcagcca cgacgtcggc ggaacagtcg tttttccagc
      241 ttatgccgga cgccttgtca caggtgttca atctgaatat gctcgcaggc acgatcgtgc
      301 cgtgccaggt gttcggcgag cgcatgaaag acaatcctga gggcggaagc atcatcaaca
      361 tttcttcgat gagcgcgctg acgccgctga cgcgcattcc cgggtacagc gcggcgaaag
      421 cggcggtgaa caacttcacg caatggcttg cagtgcattt cgcccaggag tatacggcca
      481 aactgcgggt gaacgcggtg gcgccgggtt ttttcctcac cgaacagaat cgctttctca
      541 tgaccgacga aaagaccggg gaattgacgc cgcgcggtca gaaagtgatc gaccacacgc
      601 ccatgggaga atttggcagg cccgacgacc tgattggggc ggcgatctgg ctggcctcga
      661 gtgcatcgcg atttgtaacg ggcattgtgg tgcccgtgga tggtggattt tcggcctatt
      721 cgggggtgta aatcgggccg ttggacggtg ttgaataaca acagcaagga gtttgaacac
      781 atgatcgatc cggtggcctc cctgaaagcg catcagccga atcataaatt tctgattgcc
      841 attgatagcg atggctgcgc atttgacacg atggagatca agcacaagga gtgcttcatc
      901 ccgaacatcg tcaagtactg ggaccttcag ccggtgtcaa aatacgcccg cgcagcggcg
      961 gaattcgtca acctgtattc caaatggcgc ggaatcaacc ggttcccggc gctgttgatg
     1021 gtatttgacc tgctcgacaa ctgggacaag gtccaggaac gcgatttccg gtgccccaat
     1081 gtggcgtcgt tgcgcgaatg ggtcgtcaag gagtccaagc ttggcaatcc ggtgctggag
     1141 gcctggtgca aagcgcaccc gaacaacccg gtgatggaac gtgcgctgga gtggagcaaa
     1201 gccgtcaacc gaaccgtggc ggatattgtg cagggcggcc tgccgccgtt cccctacgtg
     1261 cgggaatgcc tggaaaaggc ccacgcccgg gcagacatgc tggtctgttc gcagacgccg
     1321 accgaagcct tgacccggga atggcaggag cagggcgtgg atgcctacgt cttcgcaatt
     1381 gccggccagg aaatgggcaa gaagtccgag cacatcggat tcgcctgcca gggccgctat
     1441 gaacagacga aaatcctcat gattggcgat gccccgagcg acatgcaggc cgcgcgcgcc
     1501 aacaacgccc tctttttccc catcaacccg ggtgacgaag acaagtcgtg gaaacggctc
     1561 tacgaagaag ggctcgaccg gttttttgcg ggcgaatttg ccggggagta tgaggcttcg
     1621 ctgattgccg agtttgatac gtatcttccg gaagttccgc cctggaaaaa atagcggcga
     1681 cagcgcagcg aaacacccga ggcccggaat ctccgcggcg cctgaaggcc gggtcggcgc
     1741 ggcagctgcc gtgcccccaa acaggaagca gcacgccatg agaatcgtcg tcgatgaaaa
     1801 catgccttac gggttcgaag cattctcgac cctgggcgat gttatttcaa tgccggggcg
     1861 cagcatcgat ccggcggcgg ctgcaaatgc ggatatcctg gccattcgat ccatcaccac
     1921 agtgaacgcc gccctgcttg aaggttcgcg agtccgcttt gtggggacgg ccaccattgg
     1981 cgaggatcat gtcgacaagg cctatctcga agcgcgcggc atcggatttt ccagcgcacc
     2041 gggctgcaac gcgaacagcg tgggccagta tgtaaccgcc gccctgctcg agctggctga
     2101 ggcacacgcg ttccggctgc gaggcaccac actcggcatc gtgggcgtcg gaaatgtcgg
     2161 atcgcgcgtg ctgaaaaagg catccgccat ggggctgagc tgcgtcctca acgatccgcc
     2221 gctggaacgg aaaaccggcg attcggcata ccggccattg gatgaaatcc tggattgcga
     2281 catcgtcacg atgcacgtcc cgctgactcg cgaagggccg gatgcgacct atcatttggt
     2341 tgatgaggcc ttcgtgcgcc gcatgaaacc gggcagcatt ttgatcaaca ccgcgcgcgg
     2401 ggccgtggcg gacggagcgg ccctgaaacg ggcgcttgcg gacggccatc tgaaagcgtg
     2461 cgtgctggat gtatgggaaa atgagccgac tgttgatctt gaattgctgg aacagacctt
     2521 cataggcacg ccccacattg cgggttactc gttcgacggt aaagtgaatg gcacgcggca
     2581 gatttacgag gcggcatgcg cttttcttgg agtccctccc gcgtgggacc cctcccccct
     2641 gctccccgcg ccggaatgcc cggaggtcca tgtgaacggg gctgctgaag agcccgaagg
     2701 agtcctgcgc gacgctgtgc ggcgtgtata cgacattcgc aatgatgaca aggccatgcg
     2761 cgggctgttg cacgcgcccg agactgaaca cggtgctttt ttcgataggc tgcgcaagga
     2821 atatccgcgc cgtcgcgaat ttttcaacac gcgcgcgatc atcaccccgg agaattccga
     2881 tttggcggcc gtgttttccg gcgtgggatt tcaatgagag gctcagacag tgattgaact
     2941 gattaaagaa cacgaagcgc tgaccaatgg cgagatccag gccttcgttt ccgaggcgat
     3001 ggcatcgctt gatgccgatg gcaaacgggt gctgttcatc atccccgatc agacgcgcag
     3061 catgccgatg ccggtggtgt tccgcgctct tcacacggcc ttgagccggc gcgtgaagaa
     3121 gatggacttc cttgtcgcac ttggcacgca tccacctttg acggagacca tgctgaacac
     3181 gctgctcggt gtcacaccgg aagaacgggc aggcgtgttc gccggcgttg gcgtgttcaa
     3241 ccatgcctgg gaccagccgt cccagctggc gcgcatcgga accatttccg aggaggagat
     3301 ctatgcgatt tccgggggat tgatgcgcca gcacgttggc gtgaccctga ataaaatgat
     3361 cctggattac gacttgctgt gcgtggtcgg gccagtgttt cctcacgaag tggtgggttt
     3421 cagcggcggc aacaagtatt ttttcccggg cattgcgggc cccgacatca ttaacctgtt
     3481 ccactggctg ggggccctga tcagcaaccc cgtcatcaac ggaaccatgc acacgcccgt
     3541 gcgtgaagtg gtcaaccgcg cagcagcctg catcccggta gagaaatggt gcctggccct
     3601 gaacgttatg gggaaagact gccacggcct gttctttggc acccccgaag ccgcctggga
     3661 agccgcggct cagatgggta cccgcacgca catcgtgtac aaagataaac cgtttcacag
     3721 cgtgctggct ctggccccgg agatgtatga cgagctctgg gtagcgggca agtgcatgta
     3781 caagcttgaa cccgtggttg ccgacggcgg cgagctgatc atctacggga agcacatcaa
     3841 ggaagtcagc gtcactcacg gccactacat ccggaaaatc ggctaccaca cgcgcgatta
     3901 tttcctgtcg cagatggaga aatttaccga catccccggc ggcatcctcg cgcattcgac
     3961 gcatgtccgt ggaattggcg agatgctcga cggagtcgag aagccgcgga tccaggtcac
     4021 gcttgcaaca agcatcccga aagaagagtg cgaggccatc aatctcggct accgcgatcc
     4081 cggtagcatc gaccccgacg actggcgcgg ccgggaggat gaaggcgtgc tgctcgtgcc
     4141 gcaggcgggc gaagtgctgt atcgtctgcg cgaaggaaat ccccgcccca aagccttcac
     4201 cccctgaccc ggtgcgctca accctcagac ggctgcgcgt gctcggcacg gggtttcggc
     4261 cgggcctccg cccgcataca gaaacagatc ccgacagccc agaggatgaa ggtcagcagc
     4321 aacggataat agggcggcag gacctctttc aacaggttga atcccggggg ttgcacggcc
     4381 gatgtcgaca tagccgcagc gcctgtcagc gcgagcagcg caggcacctg ccgtattttc
     4441 aacgcaatac aataggccgc aggaagggcc agaatgtatg aacaggggac gagccgcggc
     4501 agtaccagca gatacgccac gcaaaacaac atgatgacga tcaggtagcc atcctcgcgg
     4561 aatacgtgaa gatacctgcg cactgctgcc cacgtcatca aaatcacgaa cgccacaagt
     4621 acaagatatc ccgccacggc gatcggctcg gccgccatca ttgtccccag ccttccgcac
     4681 gcagttgccg acagttcgtt gaggaggcgc caggctccgg aggatggtgc cgcacggagc
     4741 gggccaccgc catagaggcc caaaccggat ctctggaggt tctgcagaaa aatgatggtc
     4801 atttcggagt aaaacacgga cgaaaaggcc agcatagccg catacacgcc gatccccagg
     4861 aagagcgcat aatgcctccg acggccccgc cggatgaaca gcagcgcgag gaacaggatc
     4921 agggcaagtt tgaagctcgc cgacagggcc accaataccg caaaggtttt gagacggccg
     4981 cggagatacg catagagccc catccaaaga aggaactgtt caatcagacc gacgttaccg
     5041 ctggccagat cccagtacag ggcaaagttg aatccgagca ggcatatcag caaaaaaaga
     5101 ccgtcgagcg ggcgcttgag aaagtaccgg tgccacagcc agaccagtcc agcgaggcac
     5161 aggattttaa tggccagaaa cgccatatac gcggcctgaa atggaagcgc tgcaaatgga
     5221 agaaagaaat acagcgtgat cggcgggtac ccataggcac cctgcacacg cgggtcctgt
     5281 tgctggagat tcgggacgtc gtaggggtca agcccctggc tgaaagcctg cgccgcggcg
     5341 tagcaggtcc ggaacgtgag ctgaaaatgc tgtggctccc gcgcaatctt cgccgcaacc
     5401 gccaacgcat acaccacgac gagaaccgcc aggagcacgg aaacaatccc gcacggccgc
     5461 gccgcatgcg cagtgtcgtc tgacccctcc atagcagcct ttgcccccgc atttggccca
     5521 catactgtat atacactagc gcaaagcgtg attctgcgca atgggcggca tcgcctgccg
     5581 ccggcaacat gaattcgagt gcgcggctat ggtaaggtct cgtcattccg gttccttttg
     5641 tgggcccatg tggcacgttt acgcgcttca gggcgcgata tccaggggag ttgagcatgc
     5701 cgaagaatgt cattattgcg cagtccggcg gtccgagccc ggtgatcaac agttcgcttc
     5761 ggggggtcat cgaaacctgc aaaatgttcc ccggcacgtt tggcaccctc tacggtggct
     5821 ggcacggcat cgaaggcgtg ctgaaggaag aattgctcga cctttcggca caggacgaag
     5881 aagaagtcgc gctgttgcgg tacacgcccg cggcgggcag catcggcacc tgccgctaca
     5941 agctcaagtc caatcagaac gaagatttcg agcgtgtgat cgatgtcatg aaagcccacg
     6001 acatcggctt tttcttttac aacggcggca atgactcgat ggacaccgcg cataagatcg
     6061 cgcagttggc gcacgaacgc ggactggacc tcgtggccgt cggggtgccc aagacgatcg
     6121 ataacgacgt gggcgattct gaatttcagc ttatcgacca cacgcccggc tacggcagcg
     6181 tggcccgtta ctggatgagc gtagtgcaaa acgccaacga agagaactgc ggctcgtgtc
     6241 cggccgaccc cgtgctggtc cttcaggcca tgggccgaaa aatcggcttc atcccggcgg
     6301 ctgcgcggct ggcggatcca aaacgacaaa tgccgctgca gatatacatg gccgaatcga
     6361 aggtaacggt cgaggagctg tgcgataacg tcaacgacca gctcaaacgg gacgggcgct
     6421 gcatcatcgt ggtcagcgaa ggctttgaag tcggcgaact gggcgagacg aaggatagtt
     6481 tcggccacac acaattcagc gcgtcgaaga cgaccgtggc ccagcagctg gtgacgattc
     6541 tcaatgagca cggcctgccc gtgccgggca aggcgcgagg tcaggtcatg ggcaccgatc
     6601 agcgcatgac cgcgctctac gcctccatcc ccgacctgga tgaagcatat aaggtcggcc
     6661 agaaggcggc gctgatcgcc gaatcgggcg agaacggatg gatgtcgacg atactgcgcg
     6721 agcccggccc catctacaac gtgcgctacg acaaggtgcc gcttgaaaag gtcgccaatt
     6781 ccgaacgcgc gttccccaaa gcctggctgg ctccaagcaa atacgatgtc acggacgaat
     6841 tcatccggta cgcccagcca ctggtcggcg aagactgggc cagcgtgccc gtcgtgaacg
     6901 gccgccagcg attcacccgt ttcaaaccgg ttttcgccga aaagatgctg cccgaataca
     6961 ccctgcaagc cctgcgaaaa taggcgaatt gcctccggtc tctatgcgcc gaggagtttg
     7021 ccgtgcaggg cga
//
LOCUS       DAOPBQ010000140         7022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9619_length_7022_cov_4.139658, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000140 DAOPBQ010000000
VERSION     DAOPBQ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9619_length_7022_cov_4.139658"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKW60_06030"
     CDS             <1..552
                     /locus_tag="PKW60_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPJ99025.1"
                     /translation="SGSGKTTLLLILGGMLTPSSGIVRCNGVDLYAGLGAARNRFRRS
                     KIGFIFQQFHLMPYLSAYDNIRVPITLRRARPRGQIHALAERLGILDRLSHRPGQLSV
                     GEQQRVAMARTLICEPDIILADEPTGNLDAANRNIIAECLAEEHRRGRTIVLASHDER
                     LLELGSRTLELNNGRITETPLRS"
     gene            complement(591..1118)
                     /gene="hslV"
                     /locus_tag="PKW60_06035"
     CDS             complement(591..1118)
                     /gene="hslV"
                     /locus_tag="PKW60_06035"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPJ99026.1"
                     /translation="MFHATTVCAVRKGDAVALGADGQVTLGNTVMKSTATKIRRLADG
                     RVLAGFAGSVSDAISLFERFEGKLSQFNSNLSRAAVELGKEWRTDKYLRNLDALLAVC
                     DKEQSLLIAGSGEVIEPDDGILAIGSGGPFALAAARALMLHTALSAEEIVRESLLIAA
                     GICIYTNDHIRVETL"
     gene            complement(1154..2086)
                     /locus_tag="PKW60_06040"
     CDS             complement(1154..2086)
                     /locus_tag="PKW60_06040"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPJ99027.1"
                     /translation="MPDPEVQRFSEYLSVERGFSDHTARAYLNDVAQFCCYLEQGPAA
                     LASEKIDERAQPRFEILRRASRNDIRAFLGHLRTLGASPRTAARKLSSIRALYRFFVR
                     AGAMEENPAQAVHSPRLPRDLPVALSIPEVTALMEAPDAATPLGKRDRAILETLYSSG
                     VRCAELAGLTLDSIDLIGGTMVVLGKRKKERIAHLGNWALDALQAYLTVRGTLGHPGH
                     RRLFVNARGGPLTTRSVQRVMEKYVRAVLPGRRDVSPHTLRHTFATHMLNGGADLRVV
                     QEMLGHESLSTTQIYTHVSVDRLKAVYRQAHPHA"
     gene            complement(2113..4398)
                     /gene="topA"
                     /locus_tag="PKW60_06045"
     CDS             complement(2113..4398)
                     /gene="topA"
                     /locus_tag="PKW60_06045"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPJ99028.1"
                     /translation="MAKYLVVVESPAKARTINKFLGPNYVVKASYGHVRDLPKNNLGV
                     DIEKDFEPKYVALKDSRKAVKELKEAAKKVDRILLATDPDREGEAIGWHVAELLKQTD
                     KPADRVVFNEITKRCVREATRNPRPIDENLVDAQQARRILDRLVGYKISPLLQFGMQR
                     GLSAGRVQSVAVRIVCEREEAIRNFVPREYWTLDAAFLTPRGEGLTARLNRIRGEKAE
                     LENKAAVQAVLSALEGAEYHVASVERKQVKRRPYPPFITSSLQQEASRKLGFSPRRTM
                     QLAQQLYEGLAIGEEGRVGLITYMRTDSTRLAMEALDDVRQYIRENFEAAMLPGKPNF
                     YSNKKSAQDAHEAIRPTFTGHTPEAMARFLEPDQLKLYTLIWKRFVACQMTPAVFDQT
                     AIDIEARSHLFRATGSVLKFKGFLAMYEESQEDPCDEGLSGGLLPEVHEGENTRLQEF
                     KPEQHFTQPPPRFTEATLIRTLEENGIGRPSTYAQIMNTIMERGYVVREKKRLAPTPL
                     GEDVNRMLVANFPDILDIHFTAELENELDEVEEGQREWHDLLRSFYTQFERDLLAAQH
                     KIVLETVGEGTKCPECGGELEFQTGRFGPYLRCSRHPECEGRVSVTRKKVEELSDVLC
                     PECGAPMVYRRSRFGKFLGCSRYPECTHTLKKDGKEKTPAEPVGLKCPQPGCGGDLVW
                     RLGRGKRFVGCSRYPKCRYNAFGEQDLGTPCRSCGHPWTLLSKRGKQATRKCVNCGDE
                     HKETAAEAPVEESHTTEQSEV"
     gene            complement(4475..4933)
                     /locus_tag="PKW60_06050"
     CDS             complement(4475..4933)
                     /locus_tag="PKW60_06050"
                     /inference="COORDINATES: protein motif:HMM:NF016264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HPJ99029.1"
                     /translation="MKESLIELVDVILKRIQEQPERAKSESGLRKWLAGQGYKKRDID
                     AAMKLVGARIIPRSLVCQSQAVPVRTLSEAEFHKMASEARDALSRLEYYGLISPQERE
                     TILENVVQFDGRVGLDELDYLLSWIVCGNRDFESQQTIYSVMEGQADSFH"
     gene            complement(5083..6243)
                     /locus_tag="PKW60_06055"
     CDS             complement(5083..6243)
                     /locus_tag="PKW60_06055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPJ99030.1"
                     /translation="MEHPTIKKLYPVFCRNAGATHACYSLLKTMRNDSLPVEYWSSQF
                     APQLTDDFLRPGIPRTVYRGLCSLRLYEQPAVASWSARRLERRYLRSLHDRDIAYLWP
                     SVSLDLYRAIKKRGNTIVAERINCHRKTSMRVLREAYERLGWPPEHGIPAQDLEAENE
                     KMALSDYIFAPSSNVAESLLDAGIPERKLLRSSYGWEPRRFRFEDRVPSVPDCPTFLF
                     VGRGCVRKGVPWLLEMWDRAGIRGRLLLFLLGTMEKTVLQHYGHYFEREDIVLPADTS
                     NINEAYRHADVFIFPTHEEGSPLVSYEALGWGLPCLVSPMGGGEIIRDGVEGFVMDPY
                     DIERWAERIRQLAQDQALRETMARAARERAASFTWDRVGIHRRELLSSALVS"
     gene            6389..>7022
                     /locus_tag="PKW60_06060"
     CDS             6389..>7022
                     /locus_tag="PKW60_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPJ99031.1"
                     /translation="MKTVILCGGKGTRIRDVSSEIPKPMIPIGEYPVVKHIMDTYSLY
                     GFQEFILCLGFKGWKIKEYFLNFRAGTADITIDMAQNGAVTYNDAAALPPWKVTLAET
                     GLEAMTGCRIKRIQRHVGDNPFLLTYGDGVGDINVAALVEFHRSHGKLITITSVRPPA
                     RFGELVVEGNQVTSFQEKPQAAGGYINGGFFVCEPGVCDYVSDDEACTFER"
BASE COUNT         1338 a         2125 c         2110 g         1449 t
ORIGIN      
        1 agcgggagcg gcaagactac gcttctgctc atcctgggcg gtatgttgac gccttccagc
       61 ggcattgtcc ggtgtaatgg tgtcgatctg tacgccggtc tgggcgccgc ccgcaaccga
      121 ttccggcgct ccaagatcgg gttcatcttt cagcaattcc atctgatgcc gtatttgagc
      181 gcctacgaca acatccgcgt acccatcacg ctgcggcgcg cgcgtcctcg cggccaaatc
      241 catgcccttg ccgaacggct cggcatactg gaccgcttat cccatcgtcc tgggcagtta
      301 agcgtgggtg aacaacagcg cgtcgccatg gcgcgcaccc tcatctgcga gccggacatc
      361 atcctcgccg atgaacccac cggaaacctc gatgccgcga accgcaacat catcgccgaa
      421 tgccttgccg aagagcatcg gcgaggccgg accatcgtgc tcgcctcaca cgacgagcgc
      481 ctccttgaac tggggtcccg gacccttgag ctgaacaacg gacgtatcac ggagacgccg
      541 cttcgttcgt gaaccgccgc gcactgagga gtacgcagcc tgccctggaa ttagagcgtc
      601 tcgacgcgga tgtggtcgtt ggtgtagatg cagatgccgg ctgcgatgag cagggattcc
      661 cggacgattt cttcggcaga caatgccgtg tgaagcatga gggcacgggc ggcggccagt
      721 gcgaagggac ctcccgaacc gatggcaagg atgccgtcgt cgggttcgat gacttctccg
      781 ctgccggcga tgagcagcga ttgctccttg tcacacacgg ccagaagggc gtccaggttg
      841 cggaggtact tgtcggtgcg ccattcctta cccagttcca cggcagcacg ggacagattg
      901 ctgttgaatt ggctcagctt tccctcgaag cgttcgaaca gcgagatggc atcggacacc
      961 gagcccgcaa aaccagccag cacgcgcccg tcggccaggc gccggatctt ggtggcggtt
     1021 gatttcatga ctgtattgcc gagggtcacc tgcccgtcgg cgccgagcgc gacagcatct
     1081 cctttgcgaa cggcgcacac cgtggtggca tgaaacatat ccattacgac ctcctctaag
     1141 ggaaacgctt ccttcatgcg tgaggatgcg cctgccggta gactgctttc aagcgatcaa
     1201 cgctcacatg agtgtagatc tgcgtggtcg aaagactctc atgccccagc atctcctgca
     1261 ctacacggag gtcggcgccg ccgttcagca tatgtgtcgc gaaggtgtgg cgcaaggtgt
     1321 gcggagatac atcgcgccgg cccggcagca cggcccgcac gtatttttcc attacgcgct
     1381 gcacgcttcg cgtcgtgagc gggcccccgc gtgcattgac gaagagacgg cgatggccag
     1441 gatgccccaa ggttcctctg acggtgagat acgcctgcag cgcatcgagt gcccaattcc
     1501 ccagatgagc gatccgttct ttctttcgtt tgcccagcac gaccatcgtc ccgccgatga
     1561 ggtcgatgga atccagagtc aggcccgcca gttccgcgca gcgcacgccg ctggaataca
     1621 gggtttcaag aatggcgcga tcgcgtttgc ccagcggggt tgcggcatcg ggcgcttcca
     1681 tcagggcggt gacttcgggg atgctgagcg ctacgggaag gtcgcgtggc agcctgggcg
     1741 aatgaaccgc ctgagccgga ttttcttcca tggcgccggc ccgaacgaaa aaccggtaca
     1801 gcgcccgtat ggaagacagc ttccgtgcgg cggtgcgtgg agatgcgccc agggtgcgaa
     1861 gatggccaag aaacgcgcgg atatcattgc ggctggcgcg ccgcagaatt tcgaaccgcg
     1921 gttgggcgcg ttcatcgatc ttctcggaag ccaaggcagc agggccctgc tcaagatagc
     1981 aacagaactg tgcgacatcg ttgagatacg cacgcgcggt atggtctgaa aaccctcgtt
     2041 cgaccgagag gtactctgaa aaccgctgaa cctccggatc cggcatccca tccgtccttc
     2101 cggcagcccg aatcagacct cggactgttc tgtcgtgtgt gattcctcga caggcgcttc
     2161 tgcggccgtt tctttgtgct cgtcaccgca gttcacacat tttcgggtcg cctgcttgcc
     2221 ccgtttcgac agcagggtcc agggatggcc gcacgagcgg cagggcgtcc ccagatcctg
     2281 ctcgccgaac gcattgtacc ggcacttggg gtagcgcgaa cacccgacga aacgtttccc
     2341 gcggcccaaa cgccagacga gatcgcctcc gcagccgggt tgagggcatt tcagtccgac
     2401 gggctccgcc ggcgtctttt ccttcccgtc ttttttcagg gtatgtgtgc attcgggata
     2461 acgcgagcaa ccgaggaatt tcccgaaacg gcttctgcgg tacaccatgg gggcgccgca
     2521 ttcgggacag aggacatccg aaagctcttc gacttttttg cgcgtcacgc tgacgcgccc
     2581 ttcgcactcc ggatgcctgg agcagcgaag gtacgggccg aaacgcccgg tctgaaattc
     2641 gagttcgccg ccgcattcgg ggcacttcgt tccctcgcca acggtctcga gcacaatttt
     2701 atgctgcgcc gccagcaaat ctctctcaaa ctgtgtgtaa aagctccgca gcaggtcgtg
     2761 ccactcacgc tgcccttctt ccacttcgtc cagctcgttc tcgagttcag ccgtgaaatg
     2821 aatgtcaaga atgtccggga aattggccac gagcatccgg ttgacgtctt ccccgagcgg
     2881 tgtgggcgcg aggcgcttct tctcccgcac cacatagccg cgttccataa tggtgttcat
     2941 gatctgcgcg tatgtgctgg ggcgcccaat cccgttctct tcgagcgtcc ggatgagcgt
     3001 cgcctccgta aaacgcgggg gcggctgggt gaaatgctgt tccggcttga attcctgcag
     3061 acgggtgttc tcgccctcat gcacttctgg caggaggcct ccggaaagcc cctcgtcgca
     3121 cgggtcttcc tgactttcct cgtacatcgc aaggaaccct ttgaatttca gcacggaccc
     3181 cgtagcccgg aacagatggc ttcgcgcttc gatatcgatc gcggtctgat cgaacacggc
     3241 cggcgtcatc tggcaggcca caaagcgttt ccagattagg gtgtacagct tgagctggtc
     3301 gggttccagg aaacgggcca tggcctccgg ggtgtgaccc gtaaacgtcg gccgaatggc
     3361 ttcatgcgcg tcctgcgcgc tctttttgtt gctgtaaaag ttgggcttgc cgggcagcat
     3421 ggcggcctcg aaattttccc ggatatactg ccggacgtcg tcgagcgcct ccatcgccag
     3481 ccgggtcgaa tcggttcgca tgtaggtgat caaacccacg cgtccttcct cgccgatggc
     3541 aagaccttca tagagttgct gggccagctg catggtgcgg cgcggggaaa atccaagttt
     3601 gcgcgacgcc tcctgctgaa gtgaactggt gataaagggc ggatacggcc ggcgtttgac
     3661 ttgctttcgc tcgacgctcg ccacgtgata ctccgcgccc tcgagcgcgc tcagcacggc
     3721 ctgcactgcc gccttgttct cgagctcggc tttttcgccg cggatgcggt tcagccgggc
     3781 cgtcagccct tccccgcgag gcgtcaaaaa tgccgcatcc agggtccaat actcgcgcgg
     3841 aacaaaattg cggatcgcct cctcgcgttc gcaaacgatg cgcacggcta cggattgcac
     3901 gcgccccgca ctcaggccgc gctgcatacc gaattgaagc agcgggctga ttttgtagcc
     3961 taccagacga tcgagaatgc ggcgtgcctg ctgcgcatcg accaggtttt cgtcaatcgg
     4021 ccgcggatta cgggttgctt cgcggacaca acgtttggtg atttcattga acacgacccg
     4081 gtccgcgggt ttgtctgttt gtttcagcag ttcagcgaca tgccagccga tggcctcccc
     4141 ttcccggtcg gggtcggtgg ccagcaggat gcggtcaact tttttcgcgg cttccttcag
     4201 ttctttgacc gctttgcgcg aatctttcag cgcaacgtat ttgggctcaa agtccttctc
     4261 gatatccacg cccaggttgt ttttgggcag atcgcgaacg tggccatagc tggctttcac
     4321 cacatagttg ggccccagaa acttattgat ggtcctggct ttcgcaggag attcgactac
     4381 aaccagatac tttgccatgc atgactcccg atcaaagaac aacgccactt cccgacgctg
     4441 ggaagggcgt caagcaaagc ctatcgccca ccggtcaatg gaaggagtcc gcctgccctt
     4501 ccatgacact gtaaatcgtc tgttgggatt cgaaatcgcg attgccgcac acgatccagg
     4561 agagcaggta gtccagctcg tccaggccga cgcggccatc aaattgcacc acattttcga
     4621 ggatggtctc gcgttcctgc ggagaaatca gcccatagta ttcgaggcgg ctgagggcat
     4681 cgcgcgcttc ggaggccatt ttatggaact cggcctcgga cagcgtgcgc accggcaccg
     4741 cctgcgattg gcacacgagg ctgcgcggga tgatccgcgc ccccacgagt ttcatggccg
     4801 catcgatgtc gcgcttctta tatccttgtc cggcaagcca cttacgaagt cctgattctg
     4861 attttgcccg ttcgggttgt tcctggatac gtttcaggat gacatccacc aattcgatta
     4921 gggattcttt cattcaccgc tcctgatgtc ctggccggac atctcaattt gacgctctta
     4981 ttgtaggggc gattatgcct actgtcaact gaagcagagc gtcgagctcg gggaaggctg
     5041 tgcccgggca gtcgatcaac gggcataact agcgtgaaat cattaactta caagtgcgga
     5101 gcttaagagt tcccggcgat ggatgcccac gcggtcccag gtgaacgacg cagcacgctc
     5161 gcgcgcggcc cgggccatgg tttcgcgcag cgcctggtct tgggccagtt gccggatgcg
     5221 ttccgcccag cgctcaatgt cataagggtc catcacgaag ccttccacgc cgtcccggat
     5281 gatttcgccg ccacccatgg ggctcaccag gcacggcaac ccccagccca gggcttcgta
     5341 actgaccagc gggctgcctt cctcatgggt cggaaaaatg aagacgtcgg catggcggta
     5401 ggcttcgttg atgttgctgg tgtcggccgg gagcacgatg tcctcccgtt cgaagtagtg
     5461 gccatagtgc tgaagcacgg ttttttccat ggttcccaat agaaaaagga gcagacgccc
     5521 acggatgccg gcccggtccc acatttccag cagccagggc actccttttc ggacgcaacc
     5581 ccggcctacg aagagaaagg tcgggcaatc gggcacggag gggacccggt cttcgaagcg
     5641 gaaccggcgg ggctcccagc cgtagctgga acggagcaat ttccgttccg ggatgcccgc
     5701 atccagcagg gattcggcca cattgctgct gggcgcgaag atgtaatcgg acaacgccat
     5761 cttttcgttt tcggcttcga gatcctgtgc aggaataccg tgttcaggag gccagcccag
     5821 tctttcatag gcctcgcgca aaacccgcat ggaggtcttc cggtggcagt tgatccgctc
     5881 ggcgacgatc gtgtttcctc gttttttgat cgcccggtag aggtcgagcg ataccgacgg
     5941 ccagagatag gcgatatccc ggtcgtgcag gctgcgaagg tagcgccgct cgaggcgccg
     6001 cgcactccac gaagccacgg ccggttgctc atacaggcgg agggagcaga ggccgcgata
     6061 caccgtgcgc ggaatcccgg ggcggagaaa gtcgtccgtg agctgcggcg cgaactggga
     6121 agaccagtat tcgacgggca gagagtcgtt gcgcatggtt ttcagcagcg agtaacaggc
     6181 atgcgtcgcg cccgcattgc ggcagaaaac cggataaagc tttttgatcg ttggatgctc
     6241 catgacctct gtccgtatcg tgaccgcccc gccgggcgga tgaggcttcc ttgaacgtgt
     6301 tccgaccggc gtatagtatg gaagccgtcc gttgatcgca acttaccggg gcgccgggcg
     6361 gcgcccgttt ctggccaggg ggctacacat gaagaccgtg atcttgtgcg gcggaaaagg
     6421 cacacgcatc cgcgatgtgt cgagtgagat tccgaaaccg atgattccca tcggcgaata
     6481 tcccgtggtg aagcatatca tggacaccta cagcctctac ggatttcagg agttcatctt
     6541 gtgcctcgga ttcaaaggct ggaaaatcaa ggagtatttt ctgaattttc gcgcgggaac
     6601 ggcagacatc accattgata tggcgcaaaa cggcgcggtc acgtacaacg acgcagccgc
     6661 cctgcccccc tggaaagtga cgctggcgga aacaggcctc gaagcgatga ccggatgccg
     6721 tatcaagcgg attcagaggc atgtgggcga caatccgttc ctgttgacct acggcgacgg
     6781 cgtcggcgac atcaacgtgg ccgcactggt agagtttcac cggtcgcacg ggaaactcat
     6841 cacgatcaca tcggtgcggc cgccggcccg cttcggcgag ctggtggttg aggggaacca
     6901 ggtcacgtcg tttcaggaga agccccaggc cgccggaggg tatatcaacg gcggcttctt
     6961 cgtgtgcgaa ccgggggtct gcgactacgt gagcgatgac gaagcctgca cctttgagcg
     7021 gg
//
LOCUS       DAOPBQ010000141         7015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9636_length_7015_cov_3.902155, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000141 DAOPBQ010000000
VERSION     DAOPBQ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9636_length_7015_cov_3.902155"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PKW60_06065"
     CDS             complement(<1..784)
                     /locus_tag="PKW60_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPJ99032.1"
                     /translation="MKAQTTDQIGVCSWSLQATGPEDLAQKVNALGVKRVQLGLTPHR
                     DDPGAWDGVEEVLGASGIRIVTGMYSTIGEDYTTPETIRLTGGIVPDQHWEENLSLAK
                     VTAAKAAELGLPYIMAHAGFLPHDPSDPNFDKLAGRVATLARLFADAGAGLLMETGQE
                     SADTLLEFLDEMKRRGADNVAVNFDPANMILYDMDEPIEALTKLAPHVRQVHVKDAKR
                     TKVKGEWGEEVVVGTGEVDWVAFVRILANAGFDGGYIFEREAG"
     gene            1022..3775
                     /locus_tag="PKW60_06070"
     CDS             1022..3775
                     /locus_tag="PKW60_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPJ99033.1"
                     /translation="MSIRSTASRSGTALAGLGLLLASFFSGAQELSGTAVFYVAPNGN
                     DAWSGALEAPNADGTDGPFATMAGARDALRTRDAAVRAQDPVHIRIRGGNYHVTEPIT
                     FGPEDSGAACAPITYEAYPGENPVIHGGRVLSGWKTDGPLWTLDLPEVREGRWVFTGL
                     YANGEWRQPARTPNPAHPVGDYPEETDYFYTTGQVTVINPDTGKEEPSKDTIRFQAGD
                     VQQWESLPDAWVVMFHSWNTSMHFVKTLDLENHTMEFSCGSGWPMGYWQPAGQRFFIE
                     NLFEALDQPGEWYLNRKTGTLYYYPMPGETPETTVVVAPVAQQLLLLAGNPEEKSYVE
                     HLVFKGLRFNYTEYTLPRDGYPGGQAAVAVNAAVEARGARHCLIEACEIAHAGNYGVW
                     FRAGCQDNLLVRSEITDLGAGGVRIGEISSAPTAALVCMRNTVDNCFIHDGGKRYRGA
                     IGAWIGRSSYNTLSHNEICDFRYTGISVGWSWGFQPSSAHHNRIEYNRVHHTAKGQLS
                     DTGSIYTLGVSPGTVIRNNIFHDSLSYAKVSGGWGIYFDEGSTYIVAENNLVYNTLSG
                     NLHQHYGKENHVINNIFAYSHGPQLRRSREEDHVSFILERNIVYANNDSMLGVHWANG
                     NYRLESNCYWNSDGDDLDFAGRDFAEWQAEGNDAHSIVADPRFIDPEHGDFRLHPDSP
                     ALELGFQPFDLTEPGLYGDPEWVSKPGQIARDPFTPPPIPEPVAIADDFEETPENETA
                     RGAYTTEGEGAALIRVTSEAAASGTHSLKFTDIPGQKHAFLPYMHYAPRLRTGTAVGA
                     FSGRFEPGVVFFHEWRDNHSPYRVGPSLWVRGGRLIANGQTLMDLPPGVWVRFEIECA
                     LGRRATGTYGLTVTVDGQEPRHFEDLRQTGEGITRLDWFGFVSNADAETTFYIDDIAI
                     EGR"
     gene            complement(3863..5326)
                     /gene="dnaB"
                     /locus_tag="PKW60_06075"
     CDS             complement(3863..5326)
                     /gene="dnaB"
                     /locus_tag="PKW60_06075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPJ99034.1"
                     /translation="MATRPKADPVFDRTPPHDIDAERAVLGAMLLDSDAIGAGIEILR
                     ENPAEVFYAPAHQHLYGAILSLYRNNTAVDSTTLVYQLNRDGALDEAGGASYVAELTS
                     AVPTSANIEHYARIVLDAAVLRRTITICTRIIGKAYEAREDVNELLDNAEADIFAIAE
                     KRQLNPILHVGQLLQPAVEKIESIIKSHKGITGIATGFARLDELLSGFQASDMIVLAA
                     RPSVGKTALALNMARHVAVHNGQSVLVFSLEMAKEQLVQRMICMEGQIDSGKLRTGFL
                     AKQVFSKLQLAAGKLNTAPIFIDDTPNISILELRSKARRHASQHALDLIIIDYLQLMN
                     VGGRRLENRQQEISEISRAVKGLARELKVPVLALSQLSREAEKDDSGVPKLSHLRESG
                     AIEQDADVVLMLSRMTNEETEHMHQNKIRLNVAKQRNGPTGEVHLLFDKPMQRFGDLI
                     EGGGARHTEEPPPGVGEYEPDAYDTRPVDDGEDEVPF"
     gene            complement(5333..5884)
                     /gene="rplI"
                     /locus_tag="PKW60_06080"
     CDS             complement(5333..5884)
                     /gene="rplI"
                     /locus_tag="PKW60_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPJ99035.1"
                     /translation="MKVILSENVPHLGDMGATISVKGGYARNFLFPRKLAVPADSGSA
                     KELEHQRRQIARREEKVRAELAQTAKALEGLTLEIKARAGENEKIFGSVTTGVIAEKL
                     HELGHGFDRKQIELEEPIKSLGIYSVPLRLMKGITANLKVWVSAAETESAAETQSVEK
                     AAEPAEPAADTRQETADEASTEE"
     gene            complement(5961..>7015)
                     /locus_tag="PKW60_06085"
     CDS             complement(5961..>7015)
                     /locus_tag="PKW60_06085"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HPJ99036.1"
                     /translation="APSSWRARSRCCRTWRTDRGGRVAANSLVTAFRHSMALAGAGLF
                     CAVLSWAGANPVAPLPFVLLVAVYWATGRHWLGGVAVACAILIGAGATLRLQAGLFYG
                     LFVMVGALLGSGIRRQWALGTLVATVSGWMTGTFAAFLVLHLTWSGWARQASVSYEML
                     VAQAHDASGASASQQAAQEALRWLLVEHWADVSVGLMFGSFLVCAALVAAVASAWVRL
                     RYGLPGPRTTFGAMRPSEWFVWPAILCAVLWYVEQRAPNSVLRALTWNTAIGLAAIYW
                     LNGLSIAVYAMRRLQPPVFVVVAVVIFLAYVGGWMLSFAGLFDTWWEFRGKVDRLVAM
                     RDKLREGPGGGEPPGA"
BASE COUNT         1299 a         2355 c         2082 g         1279 t
ORIGIN      
        1 cgccggcctc tcgttcgaaa atgtagccgc cgtcaaagcc cgcattggcg agaatgcgca
       61 caaaggccac ccagtccact tcaccggtgc cgaccacgac ttcctcgccc cattcgcctt
      121 tgaccttcgt gcgttttgcg tctttcacat gcacctgccg cacatgcggg gccagtttgg
      181 tcagcgcttc aatgggctcg tccatgtcgt agaggatcat attggccgga tcaaagttca
      241 cggcgacatt gtccgcgccg cgccgcttca tttcatccaa aaattcgagc agggtgtccg
      301 cgctctcctg gccggtttcc atgagcaggc ccgccccggc gtctgcgaag agcctggcaa
      361 gcgtggcgac ccggccggcc agcttgtcga agttcggatc gctggggtcg tgcggcaaaa
      421 acccggcgtg ggccatgatg tagggcagcc ccagctccgc cgccttcgcc gccgtgactt
      481 tggccaggct caggttctct tcccagtgct gatccggcac gatgccgccc gtcagccgga
      541 tcgtctccgg cgtggtgtag tcctcgccaa tggtggagta catccccgtc acaatgcgga
      601 tacccgaggc gcccaggacc tcctccacgc catcccaggc gccggggtcg tcgcggtgcg
      661 gcgtcagtcc cagctgcacc cgcttgacgc caagggcgtt caccttctgc gccagatcct
      721 ccggacccgt cgcctgaagc gaccagctgc acaccccaat ctgatctgtt gtctgagctt
      781 tcactgaatc gtgcctcctt ccatcgtact tccgccgccg cgccgctgcg tcgcagtgta
      841 cccgccatgc agtgccggca acatcgaaat cagcccgcca ctcatcctag cccgctcacc
      901 gtccgaacgc aagaagtacg cattgaatat tgtctccccg tgcgtaatga tgtcgaaaac
      961 agccccacgg cgtgtcccgg ctgggtaagt gcggcgcacg tcattcagag gaggttcttt
     1021 cgtgtccata cgaagcacag catcacgttc tggaaccgcg ctggcgggcc ttggtctcct
     1081 gctggcctct ttcttcagtg gggcgcagga gctttccggc accgcagtct tctacgtcgc
     1141 acccaacggc aacgacgcct ggtccggcgc tctagaggcg cccaatgccg atgggacgga
     1201 cgggcctttt gccaccatgg ccggcgcgcg cgacgccctt cggacacgtg atgccgccgt
     1261 gcgcgcgcag gacccggttc acatccggat ccggggcggc aattaccatg tcacggagcc
     1321 gatcaccttt ggaccggaag attcgggcgc cgcgtgcgcg cccatcactt acgaggcgta
     1381 tcccggggaa aacccggtca ttcacggcgg acgcgtcctc tcggggtgga agactgacgg
     1441 cccattgtgg accctggatc tgcccgaggt gcgcgaaggc cggtgggtct tcacgggcct
     1501 ctatgccaac ggagaatggc ggcagcccgc gcgcacgccc aatcccgcgc accccgtggg
     1561 ggattacccc gaagaaacgg actatttcta caccaccggc caggtcaccg tgataaatcc
     1621 cgatacaggc aaagaagagc ccagtaaaga tacaatccgc ttccaggcgg gcgatgtgca
     1681 gcaatgggag tctctcccgg atgcctgggt cgtcatgttt cactcgtgga acacgtccat
     1741 gcatttcgtc aagacgctgg acctcgaaaa ccatacgatg gagttttcgt gcggctcggg
     1801 ctggccgatg gggtactggc agccggccgg gcagcgcttt ttcatcgaaa atctcttcga
     1861 agccctcgat cagccgggcg agtggtacct gaatcgcaag accggcacgc tgtattacta
     1921 cccaatgccg ggcgaaacgc ccgaaaccac cgtcgtggtc gcccccgtgg cccagcaatt
     1981 gctcctgctc gcgggcaacc ccgaagaaaa gagctatgtc gagcatctgg tgtttaaggg
     2041 gctgcggttc aattacaccg aatacaccct gcccagggac gggtatcccg gcggacaggc
     2101 ggctgtggcg gtcaacgccg ccgtggaagc ccgcggcgcc cgccattgcc tcatcgaagc
     2161 atgtgaaatc gcccacgccg gcaattatgg cgtctggttc cgcgccggat gccaggacaa
     2221 cctcctcgtc cgctccgaga tcacggatct gggtgcgggc ggcgtgcgga tcggcgagat
     2281 cagcagcgca cccactgccg cactcgtatg catgcgcaac acggtcgaca actgcttcat
     2341 ccacgacggc gggaagcggt accgcggcgc gattggcgcc tggatcggcc gcagctccta
     2401 caatacgcta tcccacaatg agatctgcga tttccggtac acaggcattt cggtggggtg
     2461 gtcgtgggga ttccagccga gcagcgccca ccacaaccgc atcgagtaca accgcgttca
     2521 ccacaccgcc aagggccaat tgagcgatac gggaagcatc tataccctcg gggtttcgcc
     2581 cggaaccgtg atccgcaaca acattttcca cgactcgctc tcgtatgcga aggtatcggg
     2641 aggctggggg atctatttcg acgagggctc cacgtacatc gtcgctgaga ataacctcgt
     2701 ctacaacacc ctgagcggca acctgcacca gcattacggc aaggaaaacc acgtcattaa
     2761 caacatcttc gcgtactccc acgggcccca gctccgccgg tcgcgcgaag aggatcacgt
     2821 ctcgttcata ctcgagcgca acattgtcta cgccaacaac gactccatgc tcggcgtcca
     2881 ctgggcgaac ggcaattacc ggctcgaatc caactgctac tggaattcgg acggcgacga
     2941 tctcgatttt gccgggcgcg attttgcaga gtggcaggcc gagggtaacg atgcgcactc
     3001 gattgtggct gatccgcgtt tcatcgaccc cgaacacggt gatttccggc ttcacccgga
     3061 ctctcctgcc ctggaattgg ggtttcaacc ctttgacttg acggaaccgg gactctacgg
     3121 cgacccggaa tgggtgagca aacccgggca gatcgcgcgc gaccccttca cgccgccgcc
     3181 cataccggaa cccgtcgcga tcgcggatga ttttgaagag accccggaga acgagaccgc
     3241 ccgcggcgcg tatacgacag agggtgaagg cgccgcattg atccgcgtca ccagtgaagc
     3301 tgccgcatcg ggcacgcaca gcctcaagtt cacggacatc cccggccaga aacacgcctt
     3361 cctgccgtac atgcactacg cgccgcgcct gcggaccggc acagccgttg gcgccttttc
     3421 agggcgcttt gagccgggcg tggtgttttt ccacgaatgg cgcgacaacc attcaccgta
     3481 ccgggtaggg ccaagcctct gggtgcgcgg gggccgcctc attgcaaacg ggcaaacgct
     3541 gatggacctg ccgccggggg tctgggtgcg gtttgagatt gagtgcgcgc tcggacgccg
     3601 tgccacggga acctatggcc tgaccgttac cgtggacggc caggaaccca gacactttga
     3661 ggacctgcgg cagaccgggg aaggcatcac gcgcctcgac tggttcgggt tcgtctcgaa
     3721 tgcggacgct gaaaccacgt tctatatcga cgatattgcc atcgaaggca ggtgacctcg
     3781 gggagccgcc gtacaccgcg cgacacggac acgccaccgg cgctgattct gcgcggtaac
     3841 cggcctcatc tcatgccgga actcagaagg gaacctcgtc ctcgccgtcg tctacagggc
     3901 gcgtgtcgta ggcatcgggc tcgtattctc cgaccccggg aggcggctct tccgtgtgcc
     3961 gcgctccccc gccttcgatg aggtcgccga aacgctgcat gggcttgtca aacagcagat
     4021 gcacctcgcc ggtggggccg ttgcgctgtt tggccacatt caggcggatc ttgttctgat
     4081 gcatgtgctc ggtttcttcg tttgtcatgc gcgacaacat cagcacgaca tcggcgtcct
     4141 gctcgatagc gcccgattcg cgcaggtggg agagcttggg cacgccggaa tcgtcttttt
     4201 cggcctcgcg gctcaactgc gacagggcca gcacggggac cttgagttcg cgggcgagcc
     4261 ctttgactgc gcgcgagatc tccgagattt cctgctgccg gttttcgagg cggcggcccc
     4321 cgacgttcat gagctgaagg tagtcgatga tgatcagatc gagcgcatgc tgcgatgcgt
     4381 ggcggcgcgc cttggaccgc aattccagga tgctgatgtt cggcgtgtcg tcgatgaaga
     4441 tgggagccgt gttaagtttc cccgcggcga gctggagttt tgagaagacc tgtttcgcga
     4501 ggaaacccgt gcgcaacttg cccgaatcga tctgcccttc catgcaaatc atgcgctgca
     4561 ccagctgttc cttcgccatt tccaggctga acaccagcac cgactggcca ttgtgcacag
     4621 cgacgtgccg cgccatgttc agcgcgagag ccgttttgcc caccgacggg cgcgccgcca
     4681 ggacgatcat gtccgacgcc tgaaatcccg aaagcagctc gtccagccgg gcaaaacccg
     4741 tcgcgatgcc ggtgatgccc ttgtgcgatt tgatgatgct ttcgatcttc tcgacagcgg
     4801 gctgaagcag ctgccccaca tgcaggatgg gattgagctg ccgtttttcg gcaatggcga
     4861 aaatgtcggc ttcggcgttg tcgagaagct cgttgacgtc ttcccgcgct tcgtaggctt
     4921 tgccgatgat ccgcgtgcag atggtgatcg tgcgccgaag cacggcggcg tcgagcacga
     4981 tgcgggcata gtgctcgatg ttggccgagg tcggcaccgc gctggtcagt tcggcgacgt
     5041 agctggctcc acccgcctcg tcaagagcgc cgtcccggtt gagctggtag accagcgtcg
     5101 tggaatccac ggccgtgttg ttacggtaca acgacaatat cgcgccgtag agatgctggt
     5161 gcgcgggcgc atagaagact tccgcgggat tttcgcgcaa gatctcaatc ccggcgccaa
     5221 tggcgtcgga atcgagcagc atcgccccaa gtaccgcccg ctccgcgtcg atatcgtgcg
     5281 ggggcgtacg gtcaaagacc gggtcggctt tggggcgggt cgccaccgct gtttactcct
     5341 cagtggaggc ttcgtcggct gtttcctgcc gggtatccgc ggccggctcg gccggctccg
     5401 cagccttctc gaccgattgg gtctcggccg ccgattcggt ctctgcggcg ctcacccaga
     5461 ctttgaggtt ggccgtgatg cctttcatga ggcgcagcgg taccgagtaa atgccgagac
     5521 tcttgatggg ctcttcgagc tcaatctgct tccgatcgaa cccgtgcccc agctcgtgca
     5581 atttctccgc gatcaccccg gtggtaaccg aaccgaagat cttctcgttc tcgcccgcgc
     5641 gcgccttgat ctccagggtc agcccttcga gagccttggc ggtctgcgcc agttccgcac
     5701 gcaccttctc ttcgcgccgc gctatctgcc ggcgctggtg ctccagctcc ttcgcgctcc
     5761 ccgaatcggc cggcacggcc agtttccggg ggaacagaaa attgcgggcg tagccgcctt
     5821 tgacactaat cgtggcgccc atatcgccca gatgcggcac attttcactc aggattacct
     5881 tcacggcatc cacctccctt aaagtctgca catccgccgt ccggcgggcc gccggtccgg
     5941 ctcctcacgt gtgtctgaat tcacgctccg ggcggctcgc cgccgccggg accttcgcgg
     6001 agcttatcgc gcatcgcaac gaggcgatcc actttgccgc gaaactccca ccaggtgtcg
     6061 aacaacccgg caaagctcaa catccaaccc cctacgtacg ccagaaagat gaccacagcc
     6121 accacgacga acaccggagg ttgcaggcgg cgcatcgcgt acaccgctat actcagcccg
     6181 ttgagccagt atatcgcagc cagcccgatc gcggtattcc acgtcaaggc tcgaagcaca
     6241 ctgttcggcg cccgctgttc cacataccac agcaccgcgc acaaaatcgc aggccacaca
     6301 aaccactcgg acggccgcat cgcgccaaag gtcgtgcggg ggcccggcag gccgtaccgc
     6361 aaacgcaccc acgcggacgc gactgccgcc accagggccg cgcacaccag gaacgaaccg
     6421 aacatcagtc ccacgctgac gtcagcccag tgctccacca gcaaccaccg gagcgcttcc
     6481 tgtgccgcct gctggctggc cgaagcgccg gaggcgtcgt gcgcctgcgc cacaagcatc
     6541 tcgtacgaga ccgatgcctg ccgcgcccat ccgctccagg tgagatgcag cacgagaaac
     6601 gccgcgaacg taccggtcat ccatcccgac accgtggcga ccagcgtgcc caaagcccat
     6661 tggcggcgga ttcccgaccc aagcagggcg cccaccatca caaacaggcc gtaaaacagg
     6721 cctgcctgca gccgcagggt cgcaccggcg ccaatcagta tcgcgcaggc gacggccaca
     6781 ccacccagcc aatggcgccc cgttgcccag tacacggcaa ccagcagaac aaacggcagc
     6841 ggcgcaaccg ggttggctcc tgcccaggac agcacggcgc aaaacagccc cgcgcccgcg
     6901 agcgccatgg agtggcgaaa cgccgttacg aggctgttgg ccgccacgcg cccgccccgg
     6961 tcagtccgcc acgtacggca acagcgcgat ctggcgcgcc agcttgatgg cgcgg
//
LOCUS       DAOPBQ010000142         6946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9726_length_6946_cov_3.821942, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000142 DAOPBQ010000000
VERSION     DAOPBQ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9726_length_6946_cov_3.821942"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKW60_06090"
     CDS             complement(<1..403)
                     /locus_tag="PKW60_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HPJ99037.1"
                     /translation="MMEHKARQTSAQPTRRAFLKTTGAAAAAGIAMAGQRRASGAVET
                     LAMNGGPKAVTAEFGDATKWPQYGETEEQAILELIRHPNYDPNKKLEEEWCAKFEMPY
                     SRAHFNGTSALASMFFALNLPPGSEIMVPSYT"
     gene            659..2062
                     /locus_tag="PKW60_06095"
     CDS             659..2062
                     /locus_tag="PKW60_06095"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HPJ99038.1"
                     /translation="MLFKRIPAIPFLLATILCIGAFTWGAFAAEPPAGAGWRAGAARV
                     EITPQEPIWLAGYAGRDHAAEGTLHPIWVKALALEDASEQRAVLVTTDLVGLPLAMTR
                     AVLAHAESVHGLPNSHVILCSSHTHTGPVTDNMLYEMYPLDDEQKARIERYSEELVNR
                     LIGALDEALANLGPATLESGVGVTRFAVNRRNNNEALITETHDFSGPVDHSVPVLRVA
                     RLDGTVMAVVFGYACHATTLAFYQWSGDYPGFAQIELEKAHPGATAMFFAGCGADQNP
                     LPRRTVELAHQYGQELAVAVDRVLAEPMKPLEPAFSAYYNEVELTMAPAPSREELAAL
                     AENAPDYRLRCLNQLIETLDAGQPLPSSYPYPIQLWRLGDQTLVALAGEVVVDYSVFL
                     KRMLGNDTFVMAYAHHIPSYIPSERVLEEGGYEGADAQLYYGFAAPWASGIEERIMLG
                     ATEGAAALGLPVAPWKD"
     gene            complement(2113..3378)
                     /locus_tag="PKW60_06100"
     CDS             complement(2113..3378)
                     /locus_tag="PKW60_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99039.1"
                     /translation="MATFACDVTLPLGTPIYSSYKPLETIEHPLMAKGVVIEQGNDRY
                     VLCAVDYCELRNSTYDLFREAVAESAQTSISHVAVQTVHQHTAPMGDADARPFIDAVN
                     PPPAFPANEVWDTLAATLGGAVREAMGNFTPVDAVGMSQARVDRVASNRRVPLGDGKV
                     GFRASSCRDEKIRDMPEGLIDPMLQTITFAQNGRPVARLHYYATHPQSFYGDPRASYD
                     FVGMAREHLEQEEGIFQVYFTGCAGNVAAGKYNDGSPEARQGLYERLYAAMKASCAAP
                     TFVPITPITWTTAEFPLTERDDPGYTEEDCRAQLADVSRPDSVRTGGAMGLAYRLRSN
                     RPIELNALHMGNLHIAHLPGEPMIEYQLFAKELRAGEFVAVSGYGDCAPGYICLASGY
                     GEGGYEPTASLLRPESEGPFRESIRQLLA"
     gene            complement(3669..5063)
                     /locus_tag="PKW60_06105"
     CDS             complement(3669..5063)
                     /locus_tag="PKW60_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99040.1"
                     /translation="MRLFYRISGQIVMALVVLLAPGLSFAQDDNVLQIATFRADVTPP
                     IGAVLCNGGVEPAQEIVDPLTARGVVLWAADGPIVLCAVDWVGIGNEAHDAWRDALAK
                     AAGTTRERVAVHTLHQHDTPGADYTAERFLAGNGIGGAMFDVEAAVQALNHVAAAVAE
                     AASRPERVTHLGIGMGRVEKVASNRRILGPDGQCGPARFSSCRSEELRNAPEGLIDPN
                     VRMLSFWNEDKPLVCLSYYATHPQSHYGKGGVSWDFVGIARALRDAALPGTLHVHFNG
                     AGGDIAAGKYNDGSPENRPVLAARLAEGMRLAWESTEKTPITASDVHWRTFPVSLPVS
                     EAYDMLEEGKILADEAENTPRRVRAARALAWFYRMSSGHRIDLAALDVGPATVLHMPG
                     ELCIGYQLAAQEMGPGRFVCLAAYGDYAPGYICLSEAYPQGGYEPSASRVSPEVEGVL
                     MQGMHALLQETAGE"
     gene            5634..6743
                     /locus_tag="PKW60_06110"
     CDS             5634..6743
                     /locus_tag="PKW60_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPJ99041.1"
                     /translation="MVLGWPRVAKSQTIDLAQLYQLKNAETRSISPENFTGEKGKGGM
                     ATLEEGSASRAARELGQGWKVNPYVRIEPGATFTLGEINGSGVINHIWMTPVGNYRLM
                     ILRFYWDGETEPSVEVPVGDFFAAGWGMGKEPRITSLAICINPRSGFNSYWQMPFRKS
                     CRVTMENLSEKNATVYYQIDYSLEDVSADTPYFHAQFRRVNPLPDKEVYTIVDGIRGH
                     GHYVGTYLAHGANSPGWWGEGEIKFFIDGDGEFPTICGTGEEDYFCGSYGYNERDADG
                     VYSYESFTSPYTGFHHIPNDGPQRRFGQYRWHITDPVRFKQDLRVTIQSLGWQSEGRY
                     LPLQDDLASVAYWYQLEPHNPFPKLPANEQLVIEE"
BASE COUNT         1359 a         2159 c         1992 g         1436 t
ORIGIN      
        1 acgtgtagct gggcaccatg atctcgctgc cgggcgggag gttcagcgcg aagaacatcg
       61 aggccagcgc actcgtgccg ttgaaatgtg cccgggaata gggcatctcg aacttggcgc
      121 accattcctc ttccagcttc ttattgggat cgtaattcgg atggcggatg agttcgagaa
      181 tggcctgctc ttctgtctcg ccgtattgag gccattttgt tgcgtcgcca aattccgccg
      241 tgaccgcttt cggccccccg ttcatggcaa gcgtctcgac ggctccgctg gcgcgccgtt
      301 gtccggccat cgcgatcccc gcggcagcgg cagcgcccgt ggtcttcaaa aacgcacgcc
      361 gggtcggctg cgcactcgtc tgtctcgctt tgtgctccat catccattcc tccgttttca
      421 acaaccactt ccgagttgtc ctcattgcaa gtccttgtac tctatgatat gcgcaatcac
      481 aaaacaagtt ccagcgcgcg ccggttttca ttatacatcc aatcgtgcta ctctcttttt
      541 cacgccgggc gttggtctga ttccggcaca gttcggaatg ggcgcgttgc gcgcggcgtt
      601 gagtctcgac agagattctt caccagacga ggcttctcaa caagaaggac gtatcgacat
      661 gttgtttaag cgtatacctg cgattccttt cttattggcc accattttgt gtattggcgc
      721 gttcacctgg ggcgcattcg ccgcggagcc tccggcgggc gccggctggc gcgcaggcgc
      781 cgcacgggtt gagatcaccc cacaggagcc catatggctt gcgggctatg cggggcgtga
      841 ccacgccgcc gaaggcactc ttcatccgat ctgggtgaaa gccctggcgc ttgaagatgc
      901 gtctgagcag cgcgcggtcc tcgtcacgac agacctcgtc ggcctgcctc tcgccatgac
      961 ccgggcggtg cttgcccatg cggaatccgt gcatggcctg cccaacagcc atgtcatctt
     1021 gtgcagttcc cacacccaca ccggtccggt gacggataat atgctgtatg agatgtatcc
     1081 gctggacgat gagcaaaagg cacgtattga acgctactcc gaggagcttg tgaaccggct
     1141 catcggtgcg ctggatgaag cgcttgccaa cctgggcccc gcaacactgg aatccggcgt
     1201 tggtgtcacg cgttttgccg taaaccggcg caacaataac gaagccctca ttacggagac
     1261 ccatgatttc agcggcccgg tggaccattc ggtgccggtg ctgcgggtgg ccaggctcga
     1321 cggcacggtg atggcggttg tattcggtta cgcgtgccac gcgaccaccc tggcctttta
     1381 ccagtggtcg ggagattatc caggtttcgc ccagatcgag cttgaaaaag cccatccggg
     1441 tgcgacagcc atgttttttg ccggatgcgg cgcggatcag aatcctctgc cccgccggac
     1501 tgttgagctg gcccaccaat atgggcaaga actggctgtt gccgtggacc gggtgctcgc
     1561 tgaacccatg aagcccctcg aaccggcgtt cagcgcctac tataacgagg tagaactcac
     1621 gatggcgccc gcccccagcc gggaagagct cgctgcgctg gccgaaaacg cccctgatta
     1681 ccggctgcgt tgcctgaacc agctgatcga aacactcgac gcgggccagc ctctcccctc
     1741 gtcctatccc tatccgatcc agttgtggcg cctgggcgat cagaccctgg tcgcgcttgc
     1801 aggcgaggtg gtggtcgatt actccgtctt cctgaagcgc atgctgggca acgacacgtt
     1861 cgtcatggcc tatgcccacc acatccccag ttacatcccg tcggagcggg tacttgagga
     1921 aggcggttat gaaggcgcgg acgcccagct ttactatggc ttcgctgcgc cgtgggcaag
     1981 cggtatcgaa gaacgcatca tgctgggcgc cacagaaggt gccgcggcgc tgggcctgcc
     2041 cgtagcgccc tggaaagact gaatccggct gtcaatgagt tgcgagccgt caacccgcct
     2101 gcctgcagcc ggctacgcaa gcagctgccg gatggactca cggaacggac cttccgactc
     2161 cggacggagc agcgacgccg tgggctcgta gccgccttcg ccgtatcccg aagccaggca
     2221 gatgtagccg ggcgcgcagt cgccgtaacc ggacaccgcc acgaattccc cggcacgcag
     2281 ctctttggcg aaaagctggt attcgatcat cggttcaccc ggaaggtgcg cgatgtggag
     2341 gttgcccatg tgcagcgcgt tcaattcaat gggccgattg ctccggagcc ggtatgccag
     2401 gcccatcgcg ccgcccgtgc gcacgctgtc cggcctgctc acatcagcca attgtgcccg
     2461 gcagtcttct tccgtgtagc cggggtcatc gcgctccgtc aacggaaatt ccgcggtggt
     2521 ccaggtgatg ggcgtgatcg gcacgaacgt gggcgcggcg caggaggctt tcatcgcggc
     2581 atagaggcgt tcgtaaaggc cctgacgcgc ctcgggtgag ccgtcgttgt atttccccgc
     2641 ggcgacgttt cccgcgcatc cggtgaagta cacctggaaa atgccctctt cctgctcaag
     2701 gtgttcgcgg gccatcccga caaaatcgta gctggcacgg ggatcaccgt aaaagctctg
     2761 cgggtgcgtg gcatagtaat ggagccgcgc cacggggcgg ccattctgcg caaacgtgat
     2821 ggtctgcagc atcggatcga tcagcccttc gggcatgtcc cgtatctttt cgtcgcggca
     2881 cgaactggcc cgaaaaccga ctttgccatc ccccaggggg acccggcggt ttgaggccac
     2941 ccggtctact cgcgcctggc tcatgccgac ggcatcaacc ggcgtaaagt tgcccatggc
     3001 ctctcggacc gccccgccca gtgttgcggc cagggtatcc cacacctcgt tggctggaaa
     3061 tgccggcggc ggattcacgg catcaataaa cgggcgcgca tctgcatcgc ccatgggcgc
     3121 ggtgtgctgg tgaacggttt gtacggccac atgcgagatg ctcgtctgtg cgctttcggc
     3181 cacggcctcg cggaagagat cgtaggttga attgcgcagc tcgcaataat ccacggcgca
     3241 caacacatac cggtcattgc cctgttcaat gaccacaccc tttgccataa gcgggtgctc
     3301 gattgtctcc agtggtttat agctcgagta aatgggcgtt cccagaggca gagtgacatc
     3361 gcacgcgaag gtggccaaac gggtttgtct tcccaaggct cgcggcgtgc cggttgtgga
     3421 acggcagttt gaaaccaaag cccccgcagc cagtgcggat gccccgcgca ggaatccgcg
     3481 ccgcccaatt cgtttcgact gtgcttcctt catcgtgtgc gtatcctttc cctttgctct
     3541 agccaccaat gcacagggca tttacgaaaa tcagtgccgc aatcccgaga tttccggggt
     3601 aagctgcggc atcctccatc cttcgaggaa cccggaaaaa gtcccggcca ttccctgcag
     3661 gaattatgtt actcccctgc cgtttcttgc aacaaggcgt gcatcccctg catcaagaca
     3721 ccctcgacct caggggatac ccgcgatgcc gatggttcat acccgccctg ggggtatgcc
     3781 tcactcaggc agatgtatcc gggcgcgtaa tcgccgtatg cagcaaggca gacaaagcgc
     3841 ccggggccca tttcctgtgc ggcaagctgg taaccaatac acaattcccc cggcatatga
     3901 agcacggtgg ccgggccaac atccagcgcg gccaaatcaa tccgatgccc cgaactcatg
     3961 cgataaaacc aggccaatgc ccgtgctgca cgaacacggc gcggcgtgtt ctcagcctcg
     4021 tcagccagga tttttccttc ctccaacatg tcgtatgctt cggataccgg caaggagacc
     4081 gggaacgtgc gccagtgcac gtctgatgct gttatgggtg tcttttcagt ggactcccac
     4141 gcgaggcgca tgccctccgc gagccgggcc gccagaaccg gccggttctc cggcgatcca
     4201 tcgttgtatt tgcccgcggc gatatcgcca cccgctccgt tgaaatgcac atgaagcgtt
     4261 cccggcagcg ccgcatcacg caacgcacgg gctatcccca caaaatccca ggagacgccc
     4321 ccttttccat aatggctttg cgggtgcgtg gcgtaataac tcaggcagac cagtggtttg
     4381 tcttcgttcc agaacgaaag catccgaaca ttcggatcaa tcagaccttc aggggcattc
     4441 cgaagttctt cactgcggca actgctgaag cgcgccggcc cacactgtcc gtccgggcca
     4501 aggattcgcc ggttggaggc cactttttca acgcgcccca tgccaatgcc cagatgggtt
     4561 actcgctcag gacggcttgc tgcttctgcg accgccgcag caacgtggtt cagcgcctgg
     4621 actgccgcct caacatcgaa catcgccccg ccgatgccgt ttcccgcgag aaaacgttcc
     4681 gctgtataat ccgcacccgg ggtatcgtgc tgatgcaatg tgtgtaccgc tacacgttcg
     4741 cgcgtggtgc ccgctgcttt ggccagggca tcgcgccacg cgtcgtgcgc ctcatttcca
     4801 atccctaccc agtcgaccgc acacaggacg atcggtccat ccgccgccca cagcacaacg
     4861 cctcgcgcag tcagaggatc cacaatctcc tgagccggtt cgacgccgcc attgcacaag
     4921 acggctccga tcggcggcgt tacgtccgcg cggaacgtgg caatctgcaa tacattgtcg
     4981 tcttgtgcaa aagacagccc aggcgccagc aggaccacaa gggccatcac aatttggccg
     5041 gaaatccgat agaaaagacg cattgcacct gctccttcga ttcaatttcc aggattcgaa
     5101 ccattgtcag cgctcgcggc gtcacaagcc cggatatccg ttcaggcttc gttctttccg
     5161 ccgtaaacgc cggctgtttt tcatctaaac ggatggtaac gggttgaccg gcgagattca
     5221 aaagagaaaa cagcctgttt ttcgcccgat ttcaattcag gaacgcacgc ccttgcttct
     5281 cgggacgaat caagcttttc cccagaattt cgtttgcttt gcgtaagttg gtgttttatc
     5341 agggtgtgca gcgcgtagcg caggcatctt gcctgcgttc ctctcttgtg tagctcaggc
     5401 ccctgcgcct gcccttcttc aggcggatgc gcaggcctgg gcggccaaat ggctctttca
     5461 cctggatctg caaaacagga aatcttcttg gagagtcctg tggacaaacg cattgacctc
     5521 tggtgcggca tcacgctaag gtggagtacg tacgaaacga cccatagaaa caaacaaagg
     5581 gagcggtgtc cattatgttc aagagcctct ttatcggagt gagcgtcctc tgtatggtcc
     5641 tgggttggcc gcgcgtcgcg aagtcacaga cgatcgacct ggcccagctg tatcaactca
     5701 agaatgcgga gacgcgctcc atcagccccg aaaatttcac gggtgaaaaa ggaaagggcg
     5761 gcatggccac cctggaagaa ggcagcgcca gcagggccgc ccgggagctg ggtcagggat
     5821 ggaaggtcaa cccgtatgtc agaattgagc cgggcgcgac attcactctg ggcgagatta
     5881 acggctccgg agtcatcaac cacatctgga tgacgcccgt gggcaattac cggctcatga
     5941 tcctgcggtt ctactgggac ggtgagaccg agccgtcggt ggaggtcccg gtaggcgatt
     6001 tcttcgcggc gggctggggc atgggaaaag agccccggat cacctcgctt gcgatctgca
     6061 tcaatccccg aagcggtttt aattcatatt ggcagatgcc gttccggaag agctgccggg
     6121 tcaccatgga aaatctgagc gagaaaaacg cgaccgtcta ttaccagatc gattactcac
     6181 tggaagatgt ttcggccgac acaccgtatt ttcacgccca gttccgccgg gtaaatccac
     6241 tcccggataa agaggtgtac accattgtcg acggcatccg cggccatggc cactatgtcg
     6301 gaacctacct ggcccacggc gccaacagcc cgggatggtg gggcgaaggc gagatcaaat
     6361 tcttcatcga cggcgacggc gaattcccga ccatctgcgg caccggcgag gaagattatt
     6421 tctgcggctc ctacggctac aacgagcgtg acgcggatgg cgtctactcc tacgagagct
     6481 ttaccagtcc ctataccggt ttccaccaca tccctaacga tggtccgcag cgccgctttg
     6541 gccagtatcg ctggcacatc accgaccccg tccggttcaa acaggatctg cgcgtgacca
     6601 ttcagagtct gggctggcaa agcgaagggc gttatctgcc gctccaggac gaccttgcct
     6661 ccgtcgcgta ctggtaccag ctggaaccgc ataacccatt tccgaaattg cccgcgaatg
     6721 agcagctcgt gatcgaagaa taagggtcat ttacggagat cgttgtcccg ccccggcggc
     6781 gtgcagtgaa cttcaaggtc ctggtgaagc acatcggacc cattgaacct ccgggccgca
     6841 ggggctatga tttttcgacc tcggatcgca acatcgatga accaggagac gcatttcatg
     6901 gacgctcgtt ttgcagcgat tgtgctcgta gccctgatga ttctgg
//
LOCUS       DAOPBQ010000143         6888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9812_length_6888_cov_4.740963, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000143 DAOPBQ010000000
VERSION     DAOPBQ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9812_length_6888_cov_4.740963"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="PKW60_06115"
     CDS             complement(<1..1142)
                     /locus_tag="PKW60_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HPJ99042.1"
                     /translation="MHHPSLDDFRQIARQGAIVPVYKEILADVETPVTAYIKLAKNQS
                     YSFLLESVEHADRLGRYSFLGANPSTVFRSKGTTVTIIRDGRATSFESKDPLSELRKH
                     MKEYRPIHVAGLPEFHGGAVGYISYDEVRHFAKLPDANPDPLDAPDLYFVITDTILIF
                     DHVNNRLKIVSNAHIQGDPLAAYNEAIRKIDEIEQTLRKPLILTTERIHAGTVSEEPR
                     SSFTREGFCDAVRRAKEYIKAGDALQIVLSQRFERELHASPVNVYRALRCINPSPYMT
                     LVQFPELTLVGSSPEVMTQVRGNRCKVRPIAGTRPRGVTPEEDALLEKELLADEKEIA
                     EHVMLVDLGRDDVGRVSVPGTVAPTENRLMYIERYSHVMHIVTEIEG"
     gene            complement(1157..1552)
                     /gene="hisI"
                     /locus_tag="PKW60_06120"
     CDS             complement(1157..1552)
                     /gene="hisI"
                     /locus_tag="PKW60_06120"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HPJ99043.1"
                     /translation="MATGASGKKIGNIQDLIKLDSQGLVCAVIQHHVTGQVLMVGYMN
                     REALDYTLRERKACFWSRSRQKLWLKGETSGNFLRVKELRTDCDADALLVQCEPSGPT
                     CHTNETSCFYRKVDAGGDVYLAGDGIAEN"
     gene            complement(1807..2589)
                     /locus_tag="PKW60_06125"
     CDS             complement(1807..2589)
                     /locus_tag="PKW60_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99044.1"
                     /translation="MRMIQAILWRLLCVGLLLAGATAFYLDLSTAYALLTSRLPDNTA
                     LRWALGVALIIAGLIGLIPWPKRARDSRKIAFKGAHGNVSIQLDSVEQTLNRAARKIS
                     EAKRINARITPSDNARNVHIQADVTLRKPANVSARDVEARVSAFIVDQTKRILGEETA
                     VTADLNIVDIVIEDSVAPPADAPEKKEAAPKSWLHENETPADAARETPEEESPEPPEL
                     SLPGAALLEPEYDESDDDGEDAEPASEEDEDLEAGDDKSTLW"
     gene            complement(2643..3986)
                     /gene="accC"
                     /locus_tag="PKW60_06130"
     CDS             complement(2643..3986)
                     /gene="accC"
                     /locus_tag="PKW60_06130"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPJ99045.1"
                     /translation="MFRRILIANRGEIAVRVIRACRELGIASIAVYSSVDRDCLHASL
                     ADESICIGPASATESYLHIPSIIAAAEICDAHAIHPGYGFLAENPKFARICQDCDIGF
                     IGPTPETIALSGDKAACRAKVKAAGVPVVPGSDGLIEDPAKALALAREIGFPVLIKAS
                     AGGGGKGMRIAHNDVALKKAISMASAEAETAFGDGGLYLEKFIEGARHVEFQILGDNN
                     GKIISLGERECSIQRRHQKLIEETPAPGLSSSLRARMAKAAIRAAKAIAYTNAGTAEF
                     LLTPDNQFYFIEFNARIQVEHPITEEVTGVDLVKEQIRIASGLPISNLRVRPSGYAIE
                     ARIYAEDPENGFSASPGAVPRCHLPGGPGIRVDSHLFAGYTVPRFYDSLLAKVIARGA
                     QREEAIDRLSAALQEFAADGIKTTARLCARIVSGDRFRRGDIGPDIIAQYTNGPM"
     gene            complement(4019..4489)
                     /gene="accB"
                     /locus_tag="PKW60_06135"
     CDS             complement(4019..4489)
                     /gene="accB"
                     /locus_tag="PKW60_06135"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HPJ99046.1"
                     /translation="MDVEKLQELLNIFEASGLSEIEIEEGGTRIRLKKSAPEQPGVVP
                     VYAAPAAPRLEIPVPAGAAAAAAHEPAPPEEDFVTIDSPMVGVFYAAPAPDKAPFVKP
                     GDVVQEQQTVCIVEAMKLMNEVTAQFPARIVRVLVENGEPVEFGQPLFAVEPLE"
     gene            complement(4541..5104)
                     /gene="efp"
                     /locus_tag="PKW60_06140"
     CDS             complement(4541..5104)
                     /gene="efp"
                     /locus_tag="PKW60_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPJ99047.1"
                     /translation="MISTADFRNGIVIIENGELMELVEFLHVKPGKGGAFVRTKLRNV
                     VTGRVLERTYRSGEKFEEARLVETPHQYLYSDGDLFYFMHHESFEQIPVGPDIVGDKS
                     DWLKENEEVSLLLFDGKVIALRLPITVTLKITQCDPGVQGDRASGGTKPATLETGVTI
                     QVPLFVSEGDSVKVDTRTGAYIERVST"
     gene            complement(5101..6174)
                     /locus_tag="PKW60_06145"
     CDS             complement(5101..6174)
                     /locus_tag="PKW60_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPJ99048.1"
                     /translation="MTERIEKLRAQLEAAGGDAFFSLSAPANQYLSGFTGSTSALVIT
                     PAEALFLCDFRYTEQAAGQVAGFEIDEVTGSLPTRTGERLARLNARTAVYDPGVLSVA
                     DLHEIETAFGGTLHADPGIVSSLRRVKSADEIAAIRRASALAEGVLTDLLDELTAGLP
                     ERELAARFEYEFKRRGASGASFSPIALFGPRSSLPHGAPGGTPLQPGDVVLLDFGCRL
                     DGYCSDLTRTYAFGTIPPLWFEGTYELVLTAQRIALEAAGPGMPARELDAIARSLIED
                     AGHGEHFGHGLGHGVGVEIHEAPRLNRESDTILEPGMVITIEPGVYLPGKGGIRIEDL
                     AVVTEDGCEVLSTAPKELRVIEA"
     gene            complement(6171..6605)
                     /gene="aroQ"
                     /locus_tag="PKW60_06150"
     CDS             complement(6171..6605)
                     /gene="aroQ"
                     /locus_tag="PKW60_06150"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPJ99049.1"
                     /translation="MNILVVHGPNLNLLGTREREVYGQATLEDINAMLAEEAQRHGIE
                     LRTVQSNSEGALVDAIQGAKGWADAIVMNPAAYTHTSVALRDAVSAVNIPTIEVHLSN
                     IHAREEFRQRSLIAPVAAGQIAGFGVESYRLGLLAAKSVAAR"
     gene            complement(6617..>6888)
                     /locus_tag="PKW60_06155"
     CDS             complement(6617..>6888)
                     /locus_tag="PKW60_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99050.1"
                     /translation="RYQQEPGKRRDDLINSLEQYAQSTVLFPSSPVVWENYAQALRDT
                     GANLRQAGETDQSDALQKQADEAAARAQEIRVAEYHANRRRAGLE"
BASE COUNT         1168 a         2134 c         2118 g         1468 t
ORIGIN      
        1 ccctcgattt cggtgacgat gtgcatcacg tgagaatacc gctcgatata catcaggcgg
       61 ttttccgtag gcgcgaccgt gcccggtacg ctgacgcggc ctacgtcgtc ccgacccagg
      121 tcgaccagca tcacgtgttc cgcgatctcc ttctcgtcgg ccagcagctc cttttccagc
      181 agtgcgtctt cttcgggcgt gacgccccgg ggacgcgtgc cggcgatggg ccgcactttg
      241 caccggtttc cccgaacctg ggtcatcacc tccgggctcg aacccaccag cgtcaattcg
      301 gggaattgca cgagggtcat atacggcgat gggttgatgc atcgcaacgc acggtagaca
      361 ttgaccgggc ttgcatggag ttcccgctcg aaccgttgcg acaacacgat ttggagcgcg
      421 tctccggcct tgatgtactc tttcgcccgg cgcacggcgt cgcagaatcc ttcgcgcgtg
      481 aagcttgacc gtggctcctc gctgaccgta cccgcgtgga tccgctcagt ggtcaggatc
      541 agaggtttcc gcagcgtctg ttcgatctcg tcaattttgc gtatcgcctc gttgtaggcg
      601 gccaggggat cgccctgaat gtgcgcgttc gatacgattt tcaggcgatt attcacgtgg
      661 tcgaaaatca ggatggtgtc cgtgatgaca aaatacagat cgggcgcatc cagggggtcc
      721 gggttggcgt cggggagttt cgcgaagtgc cgcacttcat catacgagat gtaccccacg
      781 gcgcctccgt ggaactcggg taaacccgcc acatggatag gccggtattc tttcatgtgt
      841 ttgcgcagtt cgctcagcgg atctttggat tcaaacgacg tggcccggcc gtcgcgtatt
      901 atcgtgaccg tcgtgccctt cgaccggaac accgtggaag ggttcgcccc caggaaggag
      961 tagcgcccga gccgatccgc atgctccacg ctttccagga gaaacgaata actctggttt
     1021 ttagcgagct tgatgtaggc ggtgaccggc gtctctacgt cggccagaat ctctttgtat
     1081 accggcacaa tggcgccctg gcgggcaatc tgccggaagt cgtccagtga cggatggtgc
     1141 atgacggttt cctcgctcag ttctcggcga tgccgtcgcc ggccaggtat acgtcgccgc
     1201 cggcatccac cttgcggtaa aaacaactcg tttcattcgt gtggcaggtg gggcctgaag
     1261 gttcgcactg caccagcagt gcgtccgcgt cgcagtcggt acgcagttcc ttcacccgaa
     1321 ggaagttgcc cgaggtttcc cccttgagcc agagtttctg ccgtgagcgt gaccagaaac
     1381 aggccttacg ttcgcgcagg gtgtagtcga gcgcttcgcg gttcatgtag ccgaccatca
     1441 ggacctggcc cgtgacgtgg tgttggataa ccgcacagac cagcccctgg ctgtccagtt
     1501 tgataaggtc ctggatgttg ccgatttttt tgccggatgc acctgtagcc atgctgttct
     1561 ctccctggat ggaaaaatca ccggaaacgg atacgctgaa cggatgaaaa cggaaagatg
     1621 cccggggagg tcaggaatag gcgcaccagc gccatcgcca gaggtggggg ctgtgcgatg
     1681 gcatcgtgct ttctcccggg gtttgcggga aacctctccc cgctgtgttc tgccgcgggc
     1741 gtagtattgc acacggtatg cccttttgca agggttttcc tgcgcgtttc cccggatgcc
     1801 ggggtgctac cagagcgtgc tcttgtcatc cccggcctcg aggtcttcgt cttcttcgct
     1861 ggccggttcg gcatcttcgc cgtcgtcatc gctctcgtcg tattccggtt cgagaagcgc
     1921 tgcgcccgga agagaaagct cgggcggctc aggcgattcc tcctcaggcg tttcccgggc
     1981 ggcgtccgca ggagtctcgt tttcgtgcag ccaggatttc ggtgcggctt cctttttctc
     2041 cggggcatcg gcgggcggcg ccacgctgtc ttcaataaca atatcgacga tgttcaggtc
     2101 ggcggtgacg gcggtctctt cgcccaggat gcgcttggtc tgatcgacga tgaacgcgct
     2161 gaccctcgct tcgacgtcgc gcgcgcttac gttggcgggt ttccggaggg tgacgtcggc
     2221 ctggatgtgg acgttccggg cgttgtccga tggtgtaatt cgcgcattga tgcgttttgc
     2281 ctcgctgatc ttgcgtgcgg cgcggttcag ggtctgttcg acggaatcca gctggatgct
     2341 cacattgcca tgggcgcctt tgaacgcgat cttgcggctg tcccgggccc gcttcggcca
     2401 ggggatcagg ccgatcaggc ccgcgatgat caacgcaacg cccagggccc accgcagcgc
     2461 cgtgttgtcg gggagccggg aggtcagcaa ggcgtaggcc gtcgacagat cgaggtagaa
     2521 ggcagtggcg ccggccaaca gcaaccccac acagagtaga cgccacagaa tagcctgaat
     2581 catacgcatg gtcgtgttcc ttcctctgtt cgcagccggt ctgccgccgc ccggtcgaag
     2641 cgttacatcg ggccgttcgt atattgagcg attatatcag ggccgatgtc tccgcggcga
     2701 aaccggtctc ccgacacgat ccgcgcgcac agccgtgccg tcgtcttgat gccgtccgcg
     2761 gcgaattcct gcaatgcggc gctgaggcgg tcaatggctt cttcgcgctg cgcgccgcgt
     2821 gcgatgactt tggccagcag cgaatcatag aaccggggca cggtgtaccc cgcgaacagg
     2881 tggctgtcga cgcggatgcc ggggccgccc ggcagatggc agcggggcac ggcgcccggc
     2941 gatgccgaaa aaccgttttc ggggtcctcg gcataaatgc gggcctcgat ggcatagccg
     3001 gacggccgga cgcgcaggtt gctgataggc aacccggaag cgatgcggat ttgctctttt
     3061 accaggtcga cgccagtgac ttcctcggtg atcgggtgtt ccacctggat ccgggcattg
     3121 aattcgatga aatagaattg attgtccggc gtgagcaaga actccgccgt gccggcattg
     3181 gtgtaggcga tggccttcgc ggcccggatc gccgcctttg ccatgcgggc gcgcagggag
     3241 ctggacagcc cgggagcagg ggtttcctcg atgagttttt gatgccggcg ctggatcgag
     3301 cattcccgtt caccgagcga gatgatttta ccgttgttat cgcccaggat ctggaactcg
     3361 acatggcgcg cgccctcgat aaatttttcg agatagaggc cgccgtctcc gaatgccgtc
     3421 tcggcttcgg cggaggccat ggaaatggct tttttcaggg cgacgtcgtt gtgcgcgatg
     3481 cgcatgccct tgccgcctcc tccggcggac gccttgatca gcacggggaa cccgatctcc
     3541 cgcgcgagcg ccagtgcttt ggccgggtcc tcgatcagac cgtcgctgcc gggaaccacc
     3601 ggcacgccgg ccgctttcac cttggcgcgg cacgccgcct tgtcgccgct cagggcgatc
     3661 gtctctggcg tggggccaat aaatccgata tcgcagtcct ggcaaatccg ggcgaatttg
     3721 gggttttctg ccagaaatcc atagccgggg tgaatggcgt gtgcgtcgca gatctctgcc
     3781 gcggcaatga tgctcgggat gtgcagatag ctttctgtgg ccgatgcggg gccgatacag
     3841 atgctttcgt cggcgagcga cgcatgcaga cagtcgcgat cgacggacga atataccgcg
     3901 atgctggcga tccccagttc ccggcaggcc cggatgacgc gtacagcgat ttcaccgcga
     3961 ttggctatca gaatgcgtcg aaacattcgt cttctcttcg gggattatgc gtcacgcctt
     4021 attcgagagg ctcgaccgcg aacagcggtt gcccgaactc gactggctcg ccgttttcta
     4081 cgagtactct cacgatccgg gccgggaatt gcgcggtcac ttcattcatg agcttcatcg
     4141 cttccacaat gcagacggtc tgctgttcct gaacgacgtc gcccggtttc acaaacggcg
     4201 ctttatccgg cgcgggcgcc gcgtaaaaca ccccgaccat cggggaatca atggtcacga
     4261 aatcttcttc cgggggggcg ggttcatgcg cggcagcggc agccgcgccc gccggtacgg
     4321 ggatttcgag gcgcggagcc gcgggcgccg catagacggg gacgacgccg ggttgttccg
     4381 gcgcactctt tttcagacgg atccgtgttc cgccttcctc gatctcgatt tcactcaacc
     4441 cagaagcttc aaaaatgttc aacagctctt gcagcttttc gacatccatc tttgcgcctc
     4501 cccgacaggg gcgtgtgccg cacgccggtt cgctctggac ttatgtgctg acccgttcga
     4561 tgtaggctcc ggtgcgtgtg tcgactttca ccgaatcccc ttcactgaca aacaacggca
     4621 cctggatggt cacgcccgtc tccagcgtgg ccggtttggt cccgccagac gcgcgatcgc
     4681 cctggacgcc ggggtcgcat tgggtgattt tcaacgtaac cgtgatgggc aggcggagcg
     4741 caatcacctt gccgtcaaac agcaacaggc tgacctcttc gttctctttc agccagtcgc
     4801 ttttgtcgcc cacgatgtct gggcccacgg ggatttgttc aaacgactca tgatgcatga
     4861 agtaaaacaa gtcgccgtcg ctgtacaggt actggtgcgg agtctcaacg aggcgcgcct
     4921 cttcgaattt ctctcctgac cggtacgttc gttccagaac ccgccccgtg accacgtttc
     4981 tcagcttggt gcgcacaaac gcgccgccct tgcctggttt cacatggagg aattccacca
     5041 gctccatgag ctcgccattt tcgataatga caatgccgtt tctgaagtcc gccgtcgata
     5101 tcatgcttcg atcaccctca actccttggg ggcggtggag agcacctcgc agccgtcttc
     5161 ggtcaccact gcaagatcct cgattcggat gccgcccttg ccaggtaagt aaactccggg
     5221 ttcgatggta atgaccatgc ccggttccag tatagtgtcg gattcgcgat tcaggcgggg
     5281 cgcttcgtgg atctccacgc ctacgccatg acccaggccg tggccaaaat gctccccgtg
     5341 tcccgcatct tcaatcaggc tgcgagcgat tgcgtccagt tcgcgggccg gcattcccgg
     5401 gcccgcggcc tcgagcgcaa tgcgctgagc agtcaatacc agctcgtacg tcccctcaaa
     5461 ccacagtggc ggaatcgtac cgaaagcata ggtccgagtc aagtcggagc agtatccatc
     5521 gaggcggcaa ccgaaatcca ggagcacgac atctcccggc tggagtggcg tgccgcccgg
     5581 cgccccatgg ggcagggaac ttcgcgggcc gaacagggca atcggggaaa acgaggctcc
     5641 cgacgcgccg cgccgtttga attcatactc gaaacgggcg gccagttccc gttccggcag
     5701 ccctgccgtc agttcgtcta gaagatccgt cagcaccccc tccgccaagg ccgaggcccg
     5761 gcgaatggcg gcaatctcgt ctgccgactt gacccggcgc aacgacgaca cgataccggg
     5821 gtccgcgtgc agcgttcccc cgaaggcggt ctcgatttcg tggagatcgg cgacgctcag
     5881 cacgcccggg tcatacaccg ctgtcctggc gttcaggcgg gccagccgtt cgcccgtgcg
     5941 cgtgggcagg cttcccgtta cctcgtctat ctcgaagccc gcgacctggc cggccgcctg
     6001 ctccgtatac cgaaaatcac agagaaatag agcttccgcc ggtgtgatca cgagggccga
     6061 cgtcgaaccc gtgaatcccg acaggtactg gttggcgggg gcggacaggc tgaaaaaggc
     6121 gtcaccaccc gccgcctcga gctgcgcgcg cagtttttcg atgcgttcgg tcatcgcgcg
     6181 gccacgcttt tggccgccag caggcccagg cggtaactct cgacgccaaa tccggcgatc
     6241 tggcccgcgg cgaccggtgc aatcagcgat cgttgccgga actcttcccg cgcgtggata
     6301 ttcgacaagt ggacctcgat cgtggggatg ttcacggcgc tgaccgcgtc gcgaagcgcc
     6361 acgctggtgt gcgtataggc ggctggattc atgacgatcg cgtccgccca gcctttcgct
     6421 ccctggatgg catcgaccag ggcgccttct gaattggact ggacggtgcg caactcgatc
     6481 ccgtgccgct gagcttcctc cgcgagcatc gcattgatgt cctcgagggt ggcctggcca
     6541 tagacctcgc gctcccgcgt cccgagcaga ttcagattcg gcccgtgtac gaccagaatg
     6601 ttcatcttgc ctcctcttat tcgagcccag cccgccggcg gttcgcatgg tactcggcga
     6661 cgcgtatctc ctgggcccgt gcggctgctt cgtccgcctg tttctgcagg gcgtcgcttt
     6721 ggtccgtctc gcccgcttgt cgaagatttg cgcccgtgtc gcgcaacgcc tgggcgtaat
     6781 tctcccagac caccgggctt gagggaaaga gtaccgtgct ttgcgcatat tgctcaaggc
     6841 tgttgatgag gtcgtcgcgc cgtttcccgg gttcctgttg atagcggg
//
LOCUS       DAOPBQ010000144         6886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9816_length_6886_cov_4.276973, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000144 DAOPBQ010000000
VERSION     DAOPBQ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9816_length_6886_cov_4.276973"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKW60_06160"
     CDS             complement(<1..249)
                     /locus_tag="PKW60_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99051.1"
                     /translation="MFFKIIFSQARYRWVFTLLLFLAMTVLVTLYVYLLNTNRFTNRA
                     MQLVMKNMGHNMFILPETANPLDTFLCTEHQPLFEDETT"
     gene            complement(322..1236)
                     /locus_tag="PKW60_06165"
     CDS             complement(322..1236)
                     /locus_tag="PKW60_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HPJ99052.1"
                     /translation="MERCIARHKGFTLIELLVVIAIIGILAAILLPALARAREAARRA
                     ACANNLKQIGLAFKMYANESKGEKWPPRMIRNIKGELSDTMIFNGPSMYPEYITDLTV
                     VWCPSMASSSPMERYDRGARGSGNKDGIIQPEELVKSPFNYTGWLYMDALNFMGFDKV
                     GIPGSGVGGRYEGTDYLNTPLGELGAANVATNGAASDEDYDYAATFPGTQAGGGSVLY
                     RLREGVERFMVTDINNPAASANSQSVVPVMWDHLTPQVMGSTHVPSGVNVLYMDGHVT
                     FSKYPGDSPWMITVDGPRIMGRYDREFQ"
     gene            complement(1442..2407)
                     /locus_tag="PKW60_06170"
     CDS             complement(1442..2407)
                     /locus_tag="PKW60_06170"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HPJ99053.1"
                     /translation="MLDQLRGYAIFGMILVNYLGHFDSMPWAFRHHKTGFSYADTIAP
                     LFVFVVGMGFRLSLLRRIQKAGAWAARWQTARRYLVLFVVAIAFYGPDARIDWWDALT
                     HIALAGLISLPFIDKNAGVRIGAAVAYLALFMLLYMGTSYGPSFMMRSMNGGPLGPLS
                     WAFVLLFGTLAYDLLSEGDVKKALLWSILGGIGLVLAGWVAWYLIPKDWAGYGSHYGA
                     FWPLSKRWCIAPAMLLSTGLCFLTFAFFYYICQVRDFQFPTLTILGHNALFIYLLQYS
                     LMEIHGAYIPDSSGVLPALLGFAGMYAFCYLAARKLYNEGIIIKL"
     gene            2406..2612
                     /locus_tag="PKW60_06175"
     CDS             2406..2612
                     /locus_tag="PKW60_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99054.1"
                     /translation="MGSRLADERAPDGFGGVCGAVAGTGTVFNGVAVMRWPQEAHAVA
                     VPIDETGMRVLWPHVQSSMRYSIN"
     gene            complement(2729..5371)
                     /locus_tag="PKW60_06180"
     CDS             complement(2729..5371)
                     /locus_tag="PKW60_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ99055.1"
                     /translation="MRRWHLCAVFMLFLAACHGPEPGGETALEQAPAARDYAQLQIKY
                     PLEGNLFPCDITAPRMHWTDPAAEASAWQVRVQFRGRDGLIEDAVDAPEWLPTAEQWE
                     AIKQHSLDTPAELIVRGIRHDDPDTPLSEGRVTFATSPDPVEAPLFYREVNLPFLEAV
                     KDPSDIRWRFGDVSSPERPPILLTNLPVCGNCHSFSADGTTLGMDVDYANDKGSYALV
                     TLSQDVVFADEDVITWSDFDRDSGEMTFGLLSQVSPCGRYAISTVKDRSVFVPRPDLY
                     FSQLFFPLKGVLAVYDRETQEYTALPGADDPQYVQSNPEWSPDGEYIVFARSTAYPLR
                     RLRDTERVLLTADECKEFLEEGKQFKFDLYRVPFNGGKGGEPVPLEGASNNGMSNFFP
                     KYSPDGKWIIFCRAESFMLLQPDSELYIIPAEGGEARRLRCNTDRMNSWHTWSPNGKW
                     LAFTSKTNGPYTQLFLTHVDENGETTPPVVLEHFSEPDRAVNIPEFVNTRPGAIRSMR
                     EQFVDDVSFERAGTEFRKAKDYLGAERQYRRALELNPNNANAHLNLGFVLDATDRPEE
                     ALESYMEALRLNPSMPEAHFNYAGTMLALGRLDEAIQEYRETIRLDPYFANAYANLAV
                     ALEKKGEPDEALALCKEAVRVDPDSSELRSNLGLTLARMGQLDEALSHLEAAVARDPD
                     SVPVQMNLGLVLSQQGRFSQAAEHYASALALEPANAEVHGHMGNLLLAQGQREEACSH
                     LLEALNLGFDNVELRRNLGLAMAQLGKSEDAITHLRKAVELAPDYAPTRVNLGAMLLS
                     QGRAEEAAEQLAAALQLDSDNLNAAVNLAEAYALAGSFDTAIPLLEKALGLARSGENA
                     SVIPEIERRIKAYKQGQPGQALPK"
     gene            complement(5613..>6886)
                     /locus_tag="PKW60_06185"
     CDS             complement(5613..>6886)
                     /locus_tag="PKW60_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPJ99056.1"
                     /translation="LKPFIYALAMDSDCLYPSESLYDGTLDYGRYAPENFDDRYHGLV
                     SAGKALQLSLNVPAVTILERVGVDRFHAFLPQAGITTLIKPAEHYGLGLTLGNCETRL
                     DEMAAAYCMIANLGLYRPLKLHEPQPQTPGTPLLTRGTCLKLFEMLEQTPPDEFAREL
                     VPVGAANTRVAWKTGTSGGYRDAWTFMFNRHYVVGVWMGNNDASPSKWLVGATVALPL
                     ATKIFRSLDVPNTPAWPETSNDLREFTVCAVSGLPATPWCPSTRRETLPDTQFLHRAC
                     DMHYPEGSGGERIVERWPGDARRWDLAAIRNPRIVDRTSPEAPQARRDALAIQHPPDG
                     AVYVLTGEPAGDRIRLRASIERTGALHWYLDDRYLGGSGPEAPLYLDLRAGNHKLTCL
                     APDGVLDSVQFEVVTPEDARRMDPKRPLIAG"
BASE COUNT         1387 a         1959 c         2183 g         1357 t
ORIGIN      
        1 cgtggtttca tcctcgaaca gcggttgatg ttcggtgcac aggaaggtgt cgagcggatt
       61 ggccgtttca ggcagaatga acatgttgtg ccccatgttc ttcatcacca gctgcatggc
      121 gcggtttgtg aagcggttgg tgttgagcag atacacgtag agcgtcacga gcaccgtcat
      181 ggcgagaaag agcagcaggg tgaacaccca acggtatcgc gcctggctga agattatttt
      241 aaagaacacg gtatctcatc cccgggcttg cccggccgct gccactcggc gaggggatac
      301 gcggcgcggc ttcgcgggcg tttattggaa ttcgcgatcg tagcggccca tgatccgcgg
      361 cccgtcaacc gtgatcatcc agggcgagtc tcccggatat tttgagaacg taacatggcc
      421 gtccatgtac agcacattga caccagacgg cacgtgggtc gagcccatca cctgcggggt
      481 gagatggtcc cacatgaccg gcacgacgct ctgtgaattt gcggacgccg cgggattatt
      541 gatgtcggtg accatgaagc gttcgacgcc ttcgcgcagc cggtagagca cgctgccgcc
      601 gccggcctgg gtgccgggaa aggtggccgc ataatcatag tcctcgtcgg atgcggcgcc
      661 attggtggcc acattggcag cccccagttc gccaagcggc gtattgaggt agtcggtacc
      721 ttcgtagcgg ccgcctacgc cgctgccggg aatgcctacc ttgtcgaatc ccatgaagtt
      781 cagagcgtcc atgtagagcc agccggtgta attgaagggg gatttgacca gctcttccgg
      841 ctgaatgatg ccgtccttat tgccggaacc gcgggcgccc ctgtcgtagc gctccatggg
      901 agagctgctc gccatggagg ggcaccagac caccgtaagg tcagtgatgt attcggggta
      961 catggacggg ccattaaaga tcatcgtgtc agaaagctcg ccctttatgt tgcgaatcat
     1021 ccgcggaggc catttttccc ccttggactc gttcgcatac attttgaagg ccagccctat
     1081 ttgtttgagg ttgttcgcac acgcggcgcg gcgggcggct tcgcgggcgc gcgcgagggc
     1141 aggcagcaga attgccgcga ggataccgat gattgcgata accacgagca gttcaatgag
     1201 tgtaaatcct ttgtgtctag cgatacatct ctccattgtc cggaacctcc gcgcgaaatc
     1261 tatgtcgacg ggtcggtgcg ccgtgggtat cccgcgccat tgaactcaag aaactttctg
     1321 aattatacgg acacggaggg agaatgacaa atgaaagtca caataaaatc agagtgacct
     1381 tcagcgcgtc actgtcgtgg cgctgatgaa ctcaggggtc gaagaagtcc cgcgggactg
     1441 gctacagttt gatgataatg ccctcattgt agagctttcg ggcagccagg tagcaaaagg
     1501 cgtacatgcc ggcaaacccg agaagggcgg gcagcacacc gctgctgtcg gggatgtatg
     1561 ccccgtgaat ctccatcagt gaatactgaa gcaggtagat gaacagcgcg ttgtggccga
     1621 gtatggtcag tgtgggaaat tgaaagtcgc gaacctggca gatgtagtaa aaaaaggcaa
     1681 aggtcaggaa gcacagccca gtcgatagaa gcatggcggg ggcgatgcac cagcgttttg
     1741 aaagcggcca gaatgcgccg taatggctgc cgtagccagc ccaatccttc gggatcagat
     1801 accaggccac ccagccggcc aggacgagtc caatgcctcc gaggatggac cagagcagcg
     1861 ctttctttac gtcgccttct gagaggaggt cataggcgag cgtgccaaag aggagtacaa
     1921 aggcccagct caatggaccc agcggcccgc cgttcatgct gcgcatcata aaggaaggcc
     1981 cgtagctggt gcccatgtag agcagcatga acaacgcgag ataggcgaca gctgcgccta
     2041 tccggacacc ggcgttcttg tcgataaacg gcagggaaat gagccccgcc agggcgatgt
     2101 gagtcagtgc gtcccaccag tctatgcggg cgtccggtcc gtaaaaggcg atcgccacga
     2161 cgaagaggac gaggtagcgg cgggccgttt gccatcgcgc ggcccaggca ccggctttct
     2221 gaatccggcg caggagcgaa aggcggaatc ccatgccgac gacgaagacg aacagcggcg
     2281 cgatggtgtc ggcataactg aaaccggttt tatgatgccg gaacgcccag ggcatggagt
     2341 caaaatgccc gagataattg acgaggatca tgccgaatat ggcataaccc cggagctgat
     2401 ccagcatggg gagccgtttg gcggatgagc gggcgccgga cggattcggc ggcgtctgcg
     2461 gtgctgtggc gggaaccggc acagtgttta acggtgtggc cgtgatgcgc tggccgcagg
     2521 aggcgcatgc cgttgccgtg cccattgacg agacggggat gcgtgtctta tggccgcatg
     2581 tgcaaagtag catgaggtat tccatcaatt aacctcccgg aaaatggtgt gttgctcctt
     2641 gacggacttg gattctgtct atgtctgcac aactacatgt acagagtcag cagctcccct
     2701 gatagtaaga gccatgtact ccccgcattt acttcggcag ggcctggcct ggctggccct
     2761 gtttatatgc cttgatcctt cgctcgatct ccggaatcac gcttgcattt tcgccagatc
     2821 gggccaaacc cagggccttt tccagcagag ggatggccgt gtcgaaacta ccggccagcg
     2881 cgtaagcctc cgccagattt acggcggcgt tcaggttatc cgagtcaagc tgcagcgccg
     2941 cggcgagctg ctccgcagcc tcttcggccc ggccctgtga caacagcatc gcacccaggt
     3001 ttacccgggt cggggcataa tcaggcgcca gttcaaccgc tttccgaaga tgcgtgatgg
     3061 cgtcctcaga cttgcccagc tgggccatgg ccaatccgag gtttcgccgc aattccacgt
     3121 tatcgaaccc aaggttcagg gcttcgagga ggtggctgca ggcttcttcg cgctgcccct
     3181 gcgcgagcag gaggttgccc atgtgcccat ggacttcggc attcgcgggc tcgagtgcaa
     3241 gcgcgctcgc atagtgttcc gctgcctgtg aaaaacggcc ctgctgggac aaaaccaggc
     3301 ccagattcat ctggacgggt acactatccg gatctcttgc gacggctgcc tccagatggg
     3361 acagggcttc atcgagttgg cccatgcgcg ccagcgtgag cccgagattg gagcggagtt
     3421 cgctgctgtc cggatcgacg cgcacggctt ccttgcacag tgcgagcgct tcgtccggtt
     3481 cgcctttctt ttccagagcc acggcgaggt tggcataggc gttcgcaaaa tagggatcca
     3541 ggcggatggt ctcacgatac tcctggatgg cctcatcgag ccggccgagc gcgagcatgg
     3601 tgccggcgta gttgaaatgc gcttccggca tcgagggatt gaggcgcaag gcttccatat
     3661 aactttcgag ggcctcttcg ggccggtccg tcgcgtccag cacaaagccc aggttcaggt
     3721 gagcattggc attgtttgga ttgagttcga gggctcggcg gtactggcgc tcggcgccca
     3781 ggtagtcttt ggcttttcga aactcggtgc cggcccgttc aaacgacaca tcatccacga
     3841 actgttcccg catgctgcgg atggcgccgg ggcgcgtgtt cacaaattcg gggatattga
     3901 ccgcgcgatc cggctccgaa aaatgttcga ggaccacggg cggggtggtc tcgccatttt
     3961 catccacatg cgtgaggaag agctgggtat acggcccatt ggtcttggac gtgaatgcca
     4021 gccacttgcc gttgggagac caggtatgcc aggaattcat ccgatcggta ttgcatcgga
     4081 gccggcgggc ctcgccgcct tccgcgggga tgatgtacag ttcgctgtcg ggttgaagca
     4141 gcatgaagct ttcggcccgg cagaagatga tccatttccc gtcaggcgag tatttgggga
     4201 agaaattgct catgccgtta ttggaggccc cttcaagcgg gacaggttcg cctccttttc
     4261 cgccgttaaa cggcacgcgg tacaagtcaa acttgaattg tttgccttcc tcgaggaact
     4321 ccttgcattc gtcggcggtg agcaatacgc gctctgtgtc gcgaaggcgc cggagcgggt
     4381 aggcggtgct tcttgcgaag acgatatatt cgccgtcagg gctccactca ggattgctct
     4441 gaacgtattg cggatcatcg gcgcccggaa gcgcggtata ctcctgcgtc tcccgatcgt
     4501 atacggcgag gactcctttg agcgggaaaa acaattgcga gaaatagaga tccggacggg
     4561 gcacaaatac agagcggtct tttacggtgc tgatggcgta acgcccgcac ggagagacct
     4621 gtgagagcag gccaaacgtc atttccccgc tgtcgcggtc gaaatcgctc caggtgatga
     4681 catcttcatc cgcgaacacc acatcctgag agagggtcac gagagcgtag gaccccttgt
     4741 cgtttgcgta gtcgacatcc ataccgagcg tggtaccgtc ggcggaaaat gaatggcaat
     4801 tgccgcacac gggcagattg gtcaagagga tgggcggccg ctcgggcgag gacacgtcgc
     4861 cgaaacgcca ccggatgtcc gacgggtctt tgacggcttc gagaaacggc aggttgacct
     4921 cgcggtaaaa cagcggggct tcgacgggat ccggcgaggt ggcgaaggtc acacgccctt
     4981 ccgagagggg cgtgtccgga tcatcgtgcc tgatgcctcg tacaatcaac tcggcgggcg
     5041 tatctaatga atgctgtttg atggcctccc actgttcggc ggttgggagc cattcagggg
     5101 catcgaccgc atcttcgatc aggccgtccc gtccgcgaaa ctgaacgcgg acttgccagg
     5161 cgctggcctc tgcagcggga tccgtccagt gcatgcgcgg ggccgtgata tcgcacggga
     5221 atagattgcc ttccagcggg tacttaatct gcagttgcgc gtaatcgcgc gccgctgggg
     5281 cctgttccag ggcagtctct ccgcctggtt cgggtccatg gcaggccgcg aggaagagca
     5341 tgaataccgc acaaagatgc caacgccgca tagcaagacc tcctcaggat cgcgcggaca
     5401 tgagaccccc gcgaatggcc gatctccctc cgcctgcgag aacagcttgc tgaccgctcc
     5461 gtgggcgtga gtataacaag gcggaaccag ctgtctccaa tgaaaaaacg agtgcaggct
     5521 gcctggaata caaagagtaa ttgaaccagg cacgtgacag gcgcctttcc ttgcgagaag
     5581 tccagggggt acccgcgacg cgaatgggtc catcacccgg caattaaagg ccgcttcgga
     5641 tccatacggc gggcatcctc gggggtgacg acctcgaact gcacgctgtc cagcacgccg
     5701 tccggcgcga gacaggtcag tttgtgattt ccagcgcgaa gatcgaggta gagcggcgcc
     5761 tccggcccgg agccgcccag atagcggtca tccaggtacc agtgcagcgc cccggtgcgc
     5821 tcgatagacg cgcgaagccg gatacgatcc ccggccggtt ctccggtcag tacgtatacg
     5881 gccccgtccg gtggatgctg gatggccagg gcgtcgcgcc gcgcctgagg cgcttccggg
     5941 gaggtccgat cgactatgcg cgggttgcgg atcgctgcca ggtcccagcg ccgggcatct
     6001 ccgggccacc gttccacaat gcgctccccg ccggaacctt cggggtaatg catgtcacac
     6061 gcgcgatgga ggaattgcgt gtccggaagc gtctcacgcc gcgtgctggg acaccacgga
     6121 gtcgccggca gtccactgac ggcgcacacc gtgaattcgc gcagatcgtt gctggtctcg
     6181 ggccaggcgg gtgtgttcgg cacgtccagc gagcgaaaga tcttggttgc cagcggcagg
     6241 gccaccgtgg cgccgaccag ccatttcgac ggcgaggcat cgttgttgcc catccagacc
     6301 cccacgacgt aatgccgatt gaacatgaag gtccaggcgt cgcgatagcc tcccgaagtg
     6361 ccggttttcc atgcgactcg cgtattggct gcgcctaccg gcaccagttc gcgcgcaaac
     6421 tcgtccggcg gggtctgttc gagcatctcg aacagcttga ggcatgtgcc gcgcgtcagg
     6481 aggggagttc ctggcgtttg cggctgcggt tcgtgcagct tgagcgggcg gtagaggccc
     6541 aggttggcaa tcatgcaata cgccgccgcc atctcatcca ggcgggtctc gcaattgccg
     6601 agcgtcaggc ccagcccgta atgttccgcg ggcttgatga gcgttgtgat tcccgcctgt
     6661 ggtaaaaatg cgtggaaccg gtcaactccg acgcgttcga ggatggtgac ggccggcacg
     6721 ttcagggaca actgaagcgc ctttccagcc gaaacgaggc catggtagcg gtcatcgaaa
     6781 ttctccggcg cgtaccgtcc gtaatcgagc gtgccgtcat acaacgactc ggaagggtag
     6841 aggcaatcgc tgtccatggc cagggcatag atgaacggtt tcaggg
//
LOCUS       DAOPBQ010000145         6885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9819_length_6885_cov_4.258126, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000145 DAOPBQ010000000
VERSION     DAOPBQ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9819_length_6885_cov_4.258126"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKW60_06190"
     CDS             <1..315
                     /locus_tag="PKW60_06190"
                     /inference="COORDINATES: protein motif:HMM:NF025255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HPJ99057.1"
                     /translation="EQPWIPWAVFAAGGVGGLLTLFLRRTIVIFASAAIGSWFVVSGI
                     LFFFAGPGALQQPSQTLLAVREGWVAVACWAVLAFAGALAQFATRAPAPQPRPRDNPP
                     RA"
     gene            411..3944
                     /locus_tag="PKW60_06195"
     CDS             411..3944
                     /locus_tag="PKW60_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99058.1"
                     /translation="MRRRANLAAYVLCWAVLAVLAAGCGTLAMLRPPVAPAGVPGVER
                     PTTVRWGSPLYGGALRTLLVLPRLGVRDAGELALRVDMTYDVVPITAGAGVDGNKASY
                     EKLHRALEAPLDLIVVANLDLTDFPVETFNGIQEKVHEGAGLLLANFGRGIPAWFQEF
                     LEGLAPVESALPITRGLAAPLTPEWQGGLDFVQAGTCGNGRVVLLDYPGHGPQYHALL
                     PELAHPESAEWAHYETYWSLIAKAALWAAHREPPLWIDRIQEETARSTGFGELPPGMT
                     EGDLREYQTAMDSRLLRAYRLYVNSPAKDTCEVMAQLRQPGRQWRTELLQKEELLKPG
                     DESYALGVPAVPGDFYLDAWIKRRGKVIDWYSIPTTIAARPFFANLRTEHEVVLPNDS
                     VRLSFELLPSQRTEDGLRRCVVVLRGTDGYGRRVAYATQDAPGARRPFAASLEVADLI
                     SPRLAVECLVVAQPAGPGSVPEFRLAGAGGARLPFGVRLSGPARPFGLAISNHGRQEY
                     GLRILNEQLAGAGVTHVVTEAFKDAARFLPETGQTPVFLLARHWTPRNIEALAGEGDM
                     PAGAPLSPVQEALARDVREVELGVADAFVLGGADGLTETYEAVRTAPPVLQEFRDRLW
                     AEFEEPAALGEAWASRFSAWDDLTLDSIQKAADAGFFEPWMRFSRHVDTVFLRLFAQA
                     REAVEESEPDTPVGFSLSTPGGGVSPLWFDSVQAMGFLAVPPERTALARVAAYRAPEL
                     ATALHVPGAASMSEARWWPWHALLNGQGGLWWNLAQGPEHVIEDTAQILPAFAAMAAH
                     ARDVRAEFADLFLRAEPLHSPVAVYDSRASEHLAAAGKTRSRSLDAALELLHATGFPA
                     RLVSPSEPGTSVFDGVQCLILPGVTVLSDAEARQIRAFVVSGGAVLADTPPGLYDEWG
                     RPRANPPLGDLFADWIAPEDLPESGEEAENGAQLENRPQALLLNRPFDSEPASDIHSA
                     AKELLERAGSAPSFRISPEQAFDGERFQFRFGEAQIFAALASPAQPGRTKYHIALPDR
                     AVVYDLRTGQELRRPGRIAWRAAPGEAALFSALPYRVTEVQAMVPATVRPGTRLNVAV
                     EVRAEERMAGTHLVQVQLRPLGRTPLPYYNKTVVCESGRSATYIPLALDEIPGFYKVV
                     VRDMLTGIVSEWPVEVASTARSVSMRIEPSS"
     gene            4040..4546
                     /locus_tag="PKW60_06200"
     CDS             4040..4546
                     /locus_tag="PKW60_06200"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006135045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPJ99059.1"
                     /translation="MKTAADQTAEFRIDHAFGPIYGQFSPRGLCSLVLPGGRHPVPPH
                     DAPRPRVNDERVRVLLAALKRYFAGEPESFDDIPLDFGPCTAFQERVWHALRDIPWGK
                     TETYGSLSQLLGYGTRASRAVGTAMGRNPIPLVVPCHRVLASQGLGGFGAGLAWKREL
                     LRIEGVEV"
     gene            4725..6662
                     /locus_tag="PKW60_06205"
     CDS             4725..6662
                     /locus_tag="PKW60_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPJ99060.1"
                     /translation="MAEEQKQPTDAQEVQEAPGMDARAADTPEEETFSIEQMEAFYDQ
                     SMASFKEGEVVRGKIVETTDDFVLVDIGYKSEGAIPIYEFPNPAAIAIGDEFDVYIEH
                     PEDEDGMPVLSKTKADKIKNWAHIQEVYEKDGVIEGRIVRRVKGGLKVDIGVDAFMPA
                     SQLTWRPTSDLDRFIGSTMEVKIIKLTKRRRNVVVSRRKLLEEQRAGEKAKLLATIEI
                     GQIIPGEVKNITDFGAFVDVGGIDGLLHVTDMSWGRVKHPSQVVSVGDKIQVMVLNFD
                     PDSERISLGLKQKTENPWKTATDRYPIGGVVRGRVVSMTDYGAFVQLEDGIEGMVHVS
                     EMSWTRRVRHPNEVMNLDEEVDVMVLNVDPEAEKIALGLKQTLPNPWREIEARYPINS
                     TVTGVVRNLTDYGAFVEIEEGIDALLHVSDISWTKKVNHPSELLERGKEIQVKILSID
                     PNNEKISVGLKQLESDPWQSVVERTPIGAHVEVEIAKLVSFGAFAKLENGVEGLIHVS
                     ELADERVQKPEEVLSVGDTVQAKVISIDPVDRKIGLSIREYKHDLEQGITSEYAASGS
                     ASVSIGEVLGDFVPKSLLRGALDPSQAANELLQEDSAAPDTAAPETSSGAEENAAETS
                     GDTPTEASGGNDPAESGPQED"
BASE COUNT         1297 a         2118 c         2251 g         1219 t
ORIGIN      
        1 gagcagccct ggataccctg ggccgtgttt gccgccgggg gtgtcggcgg cctccttacc
       61 ctgttccttc gccgaaccat cgtcattttc gcgtcggccg ccatcggttc ctggttcgtc
      121 gtctcgggca ttctcttctt ctttgccggc cccggcgccc tccagcagcc gagccagact
      181 cttctcgccg tccgcgaagg atgggtcgcc gttgcctgct gggccgtact cgcgttcgcg
      241 ggtgcgctgg cgcaattcgc cacgcgtgcc cctgcgcctc aaccccggcc ccgcgacaac
      301 cccccccggg cctgacccgg gtctgtaatg ctcccatctc cgggacgcgc ccctgcaggc
      361 agcatccggc cgatgcctcc gttcgataag gtctgataca ctatgcggcc atgagaaggc
      421 gagcaaactt agccgcgtat gtgctctgct gggcggtcct ggccgttctg gcggccggct
      481 gcgggactct ggcgatgctc cggccgcccg tggctccggc cggagtcccg ggggtggaac
      541 ggcccacaac ggtacggtgg ggcagcccgc tttatggcgg agcgctgcgc acgcttctgg
      601 tgctgccacg tcttggggtg cgggatgccg gggagctcgc gctgcgcgtg gatatgacct
      661 atgacgtggt cccaatcacc gcgggagccg gcgtggacgg aaacaaggcc tcctatgaaa
      721 aactgcaccg ggcgctggaa gcgccactcg acctgattgt agtcgcaaac ctcgatctca
      781 cggattttcc agtggagacc ttcaacggga ttcaggagaa ggtccacgag ggggctgggc
      841 tgcttctcgc gaatttcggg cgcggaatcc cggcctggtt tcaggaattt ctggaggggc
      901 tggcgccggt cgaatcggcg ctgccgatca cgcgcgggct cgctgcgccg ctgacacccg
      961 aatggcaggg cgggctcgac tttgtacagg cggggacctg tggaaatggg cgggtggtgc
     1021 tgctggatta tcctggccac ggccctcagt atcatgcatt gctgcccgaa ctcgcgcatc
     1081 cagagtccgc ggaatgggcc cattacgaaa cctactggtc cttgatcgcg aaagctgcct
     1141 tgtgggcggc acaccgtgaa ccgccactct ggattgaccg gatccaggag gaaacggcgc
     1201 gaagcacagg ttttggggag ctgcctccgg gaatgaccga aggcgacctg cgcgaatatc
     1261 agacggccat ggattcgcgg ctcctgcgcg cataccgcct gtacgtcaac agccctgcaa
     1321 aagacacctg tgaggtgatg gcacagctgc gccagcccgg ccggcagtgg cgcaccgagc
     1381 ttctgcaaaa ggaggagctc ctgaaaccag gcgacgagag ttatgccctg ggcgttccag
     1441 ccgtgcccgg ggatttctac ctcgatgcct ggatcaagcg gcggggaaaa gtcatcgact
     1501 ggtattccat tccgacaacg atcgccgctc gccctttttt cgccaatctg cggacggaac
     1561 atgaagtggt gctgcccaat gactcggtgc ggctctcgtt cgaattgctg ccatcccagc
     1621 gcaccgaaga cggcctgaga cgctgtgtcg tggtactgcg cgggacggac ggttacggca
     1681 ggcgtgtcgc ctatgcaacc caggatgcgc ccggcgcccg gcgtcccttt gcggccagtc
     1741 tggaagtagc cgacctgatc agcccccggc tcgccgtaga atgcctcgtg gttgcccagc
     1801 cggcaggacc ggggagcgtg ccggaattcc gcctggccgg tgcgggcggg gccaggcttc
     1861 cattcggcgt acggctgtca gggccggcac gcccctttgg cctggccatc agcaaccacg
     1921 gccgccagga atatggtctc cgaatactca acgagcagct ggcgggcgcg ggcgtcacgc
     1981 atgtggtaac tgaagctttc aaagacgccg cgcggtttct gcctgagacg ggccagacgc
     2041 ccgtgtttct gctggcacgc cactggaccc cgcgcaacat cgaagcgctg gcaggcgaag
     2101 gagacatgcc cgccggcgcg ccgctgtctc ccgtacagga agcgctggcc cgggatgttc
     2161 gcgaggtcga gctgggcgtg gccgatgcct ttgtgctggg tggagccgat gggttaacgg
     2221 agacctatga ggcggttcgt actgcgccgc cggtgttaca ggaattccgc gaccggctct
     2281 gggccgaatt cgaggaaccc gcggcgttgg gagaggcgtg ggcatcgcgt ttttccgcat
     2341 gggacgacct gacgctcgac agcatccaga aagcggccga tgcgggcttt ttcgagccct
     2401 ggatgcgttt cagccgccat gtggacaccg tgtttctgcg gctttttgcc caagcccgcg
     2461 aggcggtcga ggagagcgaa cccgatacgc cggtaggatt cagcctttca acgcccggtg
     2521 gcggagtatc ccctctctgg ttcgattccg tccaggcgat gggctttctg gcggttccac
     2581 cggagcggac ggccctggcg cgggttgcgg cgtaccgcgc gccggaattg gcaaccgcac
     2641 tccacgtgcc cggcgcggca tcaatgagtg aggctcgctg gtggccctgg cacgcccttc
     2701 tcaacggcca gggcgggctg tggtggaacc tcgcgcaggg accggagcat gtgattgaag
     2761 ataccgcaca gattctgccc gcgttcgccg caatggccgc gcatgcccgc gacgtgagag
     2821 cagaattcgc agacctcttc ctgcgggccg aaccgctgca cagcccggtt gccgtgtacg
     2881 acagccgcgc cagcgaacat cttgccgctg cagggaagac gcgctcccgg tcactggatg
     2941 cggcgctgga gctgctccat gccacgggct ttccagcccg gcttgtttcc ccatccgaac
     3001 cgggcacaag tgtttttgac ggcgtccagt gcctcattct cccgggcgta accgttctaa
     3061 gcgatgccga ggctcggcag attcgcgcgt ttgtggtatc cggcggcgcg gtgcttgccg
     3121 acactccccc cggtctgtat gacgaatggg gacggccgcg cgcgaacccg ccgctgggcg
     3181 acttgtttgc ggactggatc gcccccgagg acctgcccga gtccggcgaa gaagcagaaa
     3241 acggcgccca gctcgaaaac aggccgcagg cgctgctttt gaaccggcct tttgactcag
     3301 agccggcgtc agatatccat tcggcggcaa aagagctgct ggaacgggcg ggatctgccc
     3361 catcgttccg aatcagtccc gagcaggcct ttgacggcga gcgtttccag ttccgtttcg
     3421 gcgaagcaca gattttcgct gcgctggcgt cgccggctca gccgggccgt accaaatacc
     3481 atattgctct gccggaccgt gccgttgtgt acgatctgcg caccggccag gagctgcggc
     3541 ggcccgggcg cattgcatgg cgggcggccc cgggtgaggc ggctttattc tcagcccttc
     3601 cctaccgtgt aaccgaggtc caggcaatgg tgccggcgac cgtgcggccc gggacgcggc
     3661 tgaacgtggc ggtggaggtt cgcgcggaag aacgaatggc cgggacccac ctcgtccagg
     3721 ttcaattacg gccgctgggc cggacgcccc tgccgtatta caacaaaacc gtcgtgtgcg
     3781 aatcaggccg aagcgccacc tacatccccc tggccctgga cgagatcccg gggttttaca
     3841 aggttgtggt gcgcgatatg ctcacgggaa ttgtgtccga atggccggtg gaggtggcct
     3901 caactgcccg cagcgtgtcg atgcgcatcg agcccagctc ctgacgccga gcggcctttg
     3961 aattggcggg cattcgcggc gagggatttc tataataggc cgaaaaagac agcaccccgg
     4021 ctccggagga cgtgagacca tgaaaacagc agccgatcaa accgcagaat tccgtattga
     4081 ccacgctttt gggcccatct atgggcagtt ttctccacgc gggctctgtt cgctggtgct
     4141 gccgggtgga aggcacccgg tgcccccgca cgacgcgccg cgcccgagag tgaatgacga
     4201 acgcgtacga gtgctgctcg ccgcactcaa acggtatttt gcgggcgaac ccgaatcctt
     4261 tgacgacatc ccgctggatt tcggcccgtg taccgcgttt caagagcggg tttggcacgc
     4321 gctgagagat attccgtggg ggaagacaga gacctacggc agcctgtcgc aactgctcgg
     4381 ctacggcacc cgggcctccc gcgctgtcgg cacggcgatg gggcgcaatc ccattccgct
     4441 ggtcgtcccg tgccaccgtg tactggccag ccagggcctc ggcggatttg gcgcgggcct
     4501 cgcctggaaa cgcgaattac tccgcatcga aggggtcgaa gtctgacggc ctgccgcggc
     4561 ccgttacaaa gattcaaacg aggaggaggc gtcgcgccgg aagcggctat cccggccctc
     4621 ctgcgggcag atcgtatttg atcgttctcg cctgttcttg ctacactcgt ggatctgtcc
     4681 gtttgcagcg ggcaaattca cgacgaaagg atgttgtagc aaccatggca gaagaacaga
     4741 aacaaccaac agacgcacag gaggtgcagg aagcgccggg tatggacgcc cgcgccgccg
     4801 acacaccgga ggaggagacg ttttccattg agcaaatgga ggccttctac gatcagtcga
     4861 tggcgagctt caaagaaggg gaagttgtac ggggaaaaat cgtcgagacg acggacgatt
     4921 ttgtgctcgt agacatcggc tacaagagcg aaggcgccat accgatctat gagttcccga
     4981 accccgcggc catcgcgatt ggcgacgagt tcgacgtgta catcgagcat cctgaggacg
     5041 aggacgggat gccggtgctg tcgaagacca aagccgacaa gatcaagaac tgggcccaca
     5101 tccaggaggt gtacgagaag gacggagtga ttgaaggccg gatcgtgcgc cgggtgaaag
     5161 gcggcctgaa ggtcgatatt ggagtcgatg cgttcatgcc ggccagccag ttgacgtggc
     5221 ggcccacgag cgacctcgac cggttcatcg gttccaccat ggaagtgaag atcatcaagc
     5281 tcacaaaacg gcgccgcaac gtcgtggtaa gccgccgcaa actgctcgaa gaacagcgcg
     5341 cgggcgagaa agccaaactg ctggcaacca tcgagatcgg ccagatcatc cccggcgagg
     5401 tcaagaacat caccgacttc ggcgcgttcg tcgatgtcgg gggtattgat ggccttctgc
     5461 atgtgaccga catgtcgtgg ggccgggtga aacatccgtc gcaggtggtt tcggtcggcg
     5521 acaagatcca ggtgatggtg ctgaatttcg atcccgattc ggagcgcatc agcctggggc
     5581 tcaagcaaaa aaccgagaac ccctggaaaa ccgcaaccga ccgctacccg atcggcggtg
     5641 tggtgcgggg ccgcgtggtc agcatgaccg actacggcgc gtttgtgcag cttgaagacg
     5701 gcatcgaagg catggtgcac gtaagcgaga tgagctggac ccgccgcgtg cggcatccca
     5761 acgaagtcat gaatcttgac gaagaagtcg atgtgatggt gctgaacgtg gatcccgaag
     5821 cggagaagat cgcgctgggt ctcaaacaga cgctgcccaa tccgtggcgc gaaatcgaag
     5881 cgcgctaccc gatcaactcg accgtgaccg gcgtggtgcg gaaccttacc gattacggcg
     5941 cgttcgtcga gatcgaggaa gggatcgacg cgctcttgca cgtaagcgat atctcgtgga
     6001 cgaagaaggt caatcatccc tccgaactcc tcgagcgcgg gaaagagatt caggtcaaga
     6061 tcctgagcat cgacccgaac aacgaaaaaa tcagcgtcgg cctgaagcaa ctggaatccg
     6121 atccgtggca gtcggtggtt gagcgcacgc ccatcggcgc ccacgtggaa gtcgagatcg
     6181 ccaagctcgt cagcttcggc gcgtttgcca agctcgaaaa tggcgtcgaa ggcctgatcc
     6241 acgtgagcga actcgccgac gaacgcgtgc agaagccgga agaagtgctc agtgtgggcg
     6301 acaccgtcca ggcgaaggtc atcagcattg acccggtcga tcgcaaaatc ggcttgagca
     6361 tccgcgagta caagcacgac ctcgaacagg ggatcacctc cgaatacgcc gcgagcggat
     6421 ccgcgtcggt cagcatcggt gaagtcctgg gcgatttcgt gccgaaatcg ctgttgcgcg
     6481 gtgcgctgga tcccagccag gcagccaatg aacttctgca ggaagacagc gccgccccgg
     6541 ataccgcggc gccagagact tcttccggag cagaggaaaa cgccgcggaa acctccggtg
     6601 acacgcccac agaggcatcc ggtggcaacg acccggccga gtcaggtccg caggaagact
     6661 gagccatgcc cgggatgttt tggtagacag ccgttggaga gagcgttgtg ccacggcacg
     6721 acgctctctt ttgcattcct cgcgcggagt cccggcacaa cgcggagcgg cgggcggccg
     6781 cccgtgaact cagcggaact ccggcgagtc agacaattcc gaatcggggc gattcgtccc
     6841 gatccagaga gaaaccgtgc gcccgggcct ggagtcccgg cgcaa
//
LOCUS       DAOPBQ010000146         6878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9833_length_6878_cov_4.386194, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000146 DAOPBQ010000000
VERSION     DAOPBQ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9833_length_6878_cov_4.386194"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKW60_06210"
     CDS             complement(<1..530)
                     /locus_tag="PKW60_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPJ99061.1"
                     /translation="MRLDSRVPAGPIDQKWTQHKFEMRLVNPSNRRKYTVLVVGTGLA
                     GASAAATLGELGYHVKAFCIQDSARRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTVK
                     GGDYRSREASVYRLAELSVNIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARG
                     QTGQQLLLGAYSALMK"
     gene            complement(533..1237)
                     /locus_tag="PKW60_06215"
     CDS             complement(533..1237)
                     /locus_tag="PKW60_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HPJ99062.1"
                     /translation="MASNLTALVKRSIVKKQIVAVTGIILVTFIFGHLAGNFLIFAGP
                     DALNSYAEKIQGLGAFLWVVRLGLLAAFLTHISLTIWVTLENRIARGRRYEVHNTLGN
                     TTWAKRTMIFTGALVLCYVFIHLNDFTIPEKTGPSTVIPGVNNDESLGLFGLVWNSFL
                     NPLRAGFYILAVSFLGMHLSHGIESVFQSLGTPLGQQRPTLLKISRIIGLLLALSFSS
                     IPIYVIVRHYTIGAGV"
     gene            complement(1509..2420)
                     /locus_tag="PKW60_06220"
     CDS             complement(1509..2420)
                     /locus_tag="PKW60_06220"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPJ99063.1"
                     /translation="MNATTPPCGIITVLNTPFTQDDAIDVYGLARNVENAVAAGVAGF
                     LAPAMASEVDKLTENERDVMVRTVLEAAAGRVPVFGGASAASQAERVHWTRAMLRLGC
                     DGVLVSVPFEDEAQYEREVREIAECGPPMLMLQDWDPRGYGVPVAVIARLFDELECFR
                     SLKIEVVPAGVKYSDVLHATDGRLHVAGGWAVMQMIEALDRGVHAFMPTGMHRLYTRI
                     YALHATGRRAEARRLFEQILPVLAFSNQHLDISIHFFKRLLHAQGVYETARVREPILP
                     FDAAHERIAAELIERALALEDGLCAAG"
     gene            2632..3567
                     /locus_tag="PKW60_06225"
     CDS             2632..3567
                     /locus_tag="PKW60_06225"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPJ99064.1"
                     /translation="MSNDSYRYIFGPVVSRRLGRSLGVDVLPPKTCTYDCVYCQLGRT
                     THQTSERGAFVPLEDLLSELRRKLQEGCAVDFITVVGSGEPTLYSRLGEFIAAAKNLA
                     GVPVAVITNGSLLWRPEVRAELLQADLVIPSLDAGDAAMFHEINRPCAGIAFEQMVEG
                     LVGFRREYSGQLWLEVLLLDGVTDGQVERIKALAERIQPDRIQLNTAVRPRAFSAVRP
                     LSAEALRRIVAALGDRAELVAAVEAADDSATRAATPGEVLNMLRRHPASVRETALHLS
                     ITEQNARECIDALLNSGDIEAHRLETGICYRARSA"
     gene            3599..5464
                     /locus_tag="PKW60_06230"
     CDS             3599..5464
                     /locus_tag="PKW60_06230"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HPJ99065.1"
                     /translation="MHENTVRVIFQPYGRIVRVPAGSTVREAATLAGIPLEYPCGGQG
                     TCGKCRVKMPGAQSPHTAAELSLLSGDERGAGTRLACQTRVHENVTVEIPESSMIQGK
                     NQILTGRGASASPVLDPPVRSIPVKMPVPTLTDDRPDAERLLESVPADSIAPALLYEL
                     PGLLRDHGFQGTAIVASGCVIDFAGGAAEPPCLAAAFDIGTTTLVGELLDLRTGAICG
                     SASRMNPQTIFGDDVLSRISHASRGPEALQEVHNTVVSALNDMLAEMSAQAEASVENI
                     YEISVSGNTTMEHFFAGLNPGALGMVPFVPVARRGLTLDPGQAGVAMNPRGRVFVLPV
                     IGGFVGGDTVAGIAATRLEESERPALLIDIGTNGELALIHNGGLSATSCAAGPAFEGA
                     RISCGMRAAAGAIEAVELGDDVQSKTIGGVPPIGLCGSGLIDLVAELLRKGILQSQGL
                     FTPRDALPDDLPPALRDRVVDHAEGAAFVLARADETRMQRPLLFTQRDVRELQLAAGA
                     IRAGISILLKRADLEPAELGHVYVAGGFGNYIRLVNAQRIGLLPGALREEQFSFAGNT
                     SLEGARAAALSLGARARAEAISSSTRHFDLSCDPEFQTEFAMAMFFPDSSSVETV"
     gene            5607..>6878
                     /locus_tag="PKW60_06235"
     CDS             5607..>6878
                     /locus_tag="PKW60_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPJ99066.1"
                     /translation="MDTQFTALMKNSSDAVLVIDASSGNVVECNEAALRLLKTPADRI
                     LGRHHTALYPPEMREDCAQRLKQIAAGTPANAVESQLQQGDGGTAWVEMSGSACEAHG
                     RILVLNAFRDMTGLRRAKEENRRLKRILLAINACDEAIMEASSESQLLARVTESLVET
                     GVCDIAALVCFHSDGPAIAAQHSRDGLAVLAADLPSLCGVLGWPSRGKAAQPDMKVAR
                     FETEGSSPAFSWAVASGESPLCLVSFPFDCAGQEQGALFLSSFAHGAFDPQELEMLGR
                     VPDHLRNGIETIRTRSERDQALEDLQFRLGLEAILSQAIREFFNLAEQGVDASIQAAW
                     GRISEFLGLDHSFCVIFEKGDSPGASFEWHAPVLDSLPPEVSALVLATLSRHSSPELN
                     RGEPLMITAGIPEFADRRVEQDLCEVLEARCV"
BASE COUNT         1260 a         2115 c         2182 g         1321 t
ORIGIN      
        1 tctttcatca gcgcgctgta ggcgccgagg agcagctgtt gtcccgtctg gccgcgcgcg
       61 tagaaggttc gtgacacctg agcgccgcca aacgagcggt tgtcgaggag accgccgtat
      121 tcacgcgcga acggcacgcc ctgcgcaacg cattgatcga tgatgttgac gctcagctcc
      181 gccaggcggt acacgctggc ttcccgggat cggtagtccc cgcctttgac cgtgtcgtag
      241 aagagccggt agacgctgtc gccgtcgttc tgatagttct tcgccgcgtt gataccgccc
      301 tgggcggcga tgctgtgggc gcgccgcgcc gagtcctgga tgcagaaggc cttgacgtgg
      361 tagccgagct cgccgagggt ggccgctgcc gaggcgcccg ccaggcccgt gcccaccacg
      421 agcaccgtat attttcggcg gttcgaggga ttcaccagcc tcatctcgaa cttgtgctgc
      481 gtccatttct gatcgatggg ccccgcgggg actctggaat ccaaacgcat gattacactc
      541 ccgcgccaat ggtgtaatga cggacaatca cgtagatggg aatcgaactg aagctcaggg
      601 ccaggagaag cccgataatc cggctgatct tcaagagcgt ggggcgctgc tgccccaggg
      661 gagtgcccag cgactggaac acgctttcga tgccgtggct caggtgcatc ccgaggaacg
      721 acacagccag gatgtagaaa cccgcacgga gcgggttcag gaacgagttc cagaccagtc
      781 cgaacaggcc caggctttcg tcgttgttga cccccgggat gaccgtcgaa ggccctgtct
      841 tttcaggaat agtgaaatcg ttgaggtgga tgaaaacgta acagagcacc agcgccccag
      901 tgaagatcat cgtccgcttg gcccaggtgg tattgcccag cgtattgtgg acttcgtagc
      961 gtctgccccg cgcgatacga ttttcaagcg tgacccagat ggtgagcgag atgtgcgtca
     1021 ggaaggcggc cagcagcccc aggcggacca cccacagaaa cgcgcccagc ccctggatct
     1081 tctcggcgta gctgttcagg gcatccggcc ccgcgaaaat caggaaattg cctgccagat
     1141 ggccgaagat gaacgtcacc aggatgatgc ccgtcaccgc gacaatctgc ttcttgacga
     1201 tcgatcgttt caccaatgca gtcaaattcg aagccatgag catatcctgg tttatacgcc
     1261 tcggagctga atcgaatctc cggggcttcg ccttgatctc caccgctcat ttgactatag
     1321 cataccgcca ggtctgcaca caccgggtca ggcgcgcact tgctggcgca tggagcctct
     1381 ggattgggcg aattctactt tatttggcgc ctgggatggc accaaacagg cgggaaaagc
     1441 gagtccagcg gcggatccgg cgctgccttt ggcgcgcatg gcatggcggg cgcacgcgtg
     1501 gcgccccgtc accccgctgc gcacaagccg tcctccaggg ccagggcacg ttcgatcagc
     1561 tcggctgcga ttcgctcgtg tgcggcatcg aacggcagaa tgggctcacg aacacgcgcg
     1621 gtctcgtaca ccccctgggc atgcagcagg cgtttgaaaa aatggatcga gatgtccaga
     1681 tgctgattgg aaaatgcgag caccggcaag atctgttcga agagccgccg tgcttccgct
     1741 cgcctgcccg ttgcatgcag cgcgtaaatg cgtgtataga gccgatgcat ccccgttggc
     1801 atgaacgcgt gtacgcctcg atccagcgct tcaatcatct gcatcactgc ccatcctccc
     1861 gccacgtgca gccgcccgtc cgtggcatgc aggacgtcac tgtatttcac tcccgccgga
     1921 acgacctcga tcttcaatga gcggaagcac tcaagttcat cgaacaggcg cgctatcacc
     1981 gcgaccggca cgccataacc gcgcggatcc cagtcctgaa gcatgagcat gggcggcccg
     2041 cattctgcaa tttcgcgtac ctcacgttca tattgcgcct catcctcaaa cggcacgctc
     2101 accagcacac cgtcgcagcc caggcggagc attgcccggg tccaatggac gcgttccgcc
     2161 tgggaggccg ccgatgctcc cccgaacacc ggcacgcggc ccgctgcggc ctcaagcacg
     2221 gtgcgcacca tcacgtcgcg ttcgttttcg gtcagcttat ccacctcgct cgccatggcg
     2281 ggcgccagaa accccgccac ccccgcagcg acagcgttct cgacatttcg agccagtccg
     2341 taaacatcga tcgcatcatc ctgagtaaat ggcgtgttca aaacggtgat gattccgcag
     2401 ggaggagttg tggcgttcat ccgtgttcct ttcgtcaggc ccgtccgggg cgcttgaaag
     2461 gctaagcata ccctatgtgc gcctcagcgc gggttcgaac ggggacgagc gcccgtgctg
     2521 caagcggctg tggcggctat ggctctctgt ctccggcccc ggtctccgtg ataccatagg
     2581 gccccacccg tatgtgccgg atacggatgg ccaagctcgg aaatctgaac gatgagcaac
     2641 gacagctacc gttacatttt tgggcccgtt gtgtcgcgcc gtctcgggcg cagcctgggc
     2701 gtggatgtgc tgccgccgaa gacatgcacg tatgactgcg tgtattgcca actgggccgc
     2761 accacgcacc agacgtccga acgtggggcg tttgtgccgc tcgaggacct gttgtccgag
     2821 ttacggcgaa aactccagga aggctgcgcc gtcgatttca tcacggtcgt gggttcgggc
     2881 gaacccactc tgtacagccg gctcggcgaa tttatcgccg ctgcgaagaa cctggccggc
     2941 gttccggtgg cggttattac gaacggctcg ctgctctggc ggcctgaggt tcgggcggag
     3001 cttctgcagg ccgatctggt gattccgtcg cttgatgcgg gcgatgcggc catgtttcat
     3061 gagattaacc gcccctgcgc cggcattgct tttgaacaga tggtcgaagg actggtgggg
     3121 ttccggcgcg aatattccgg gcaactctgg ctggaagtgt tgctcctgga cggggtgacg
     3181 gacgggcaag tcgagcgcat caaggcgctt gcggagcgca tccagcccga ccgcattcag
     3241 ctgaacacgg cggtgcgtcc gcgcgctttt tcggccgtgc ggcccctgtc cgccgaagca
     3301 ttgaggcgga ttgtcgcagc cctgggagat cgcgccgagc tggtagcggc ggtagaggct
     3361 gcggacgatt ccgcaacccg tgcggcaacg cccggggaag tattgaacat gctgcgccgg
     3421 catcccgcat ccgtgcggga aaccgctctg catctctcca ttacggagca gaatgcccgg
     3481 gaatgcatcg acgctctcct gaattccgga gatatcgagg cgcaccggct cgaaactgga
     3541 atctgttacc gcgcgcgaag cgcctgaacc aatggacggg tgaattgaag ggttgcctat
     3601 gcacgaaaat acagtgcgag tcatttttca accgtatggc cggatcgtgc gagtacccgc
     3661 cggcagcacg gtgcgcgaag cggctacgct ggccggtatt ccgctggaat atccctgcgg
     3721 ggggcaaggc acctgcggca aatgccgggt gaagatgccg ggcgcccaaa gcccccacac
     3781 tgccgccgag ctgagcctgc tgtctggcga cgaacggggc gcggggaccc ggctggcctg
     3841 ccagactcgc gttcatgaaa acgtcacggt tgagattccg gaaagcagca tgatccaggg
     3901 aaaaaaccag atcctcacgg gacgcggcgc ttcagcgtcg ccggtgctcg atccgccggt
     3961 ccgatccatc cccgtgaaga tgcccgttcc aaccctgacc gatgatcgcc ccgacgcgga
     4021 acgccttctc gaatccgttc cggcggactc catcgctccg gcgctcctgt acgagcttcc
     4081 cgggttattg cgcgatcatg gttttcaagg cacagccatt gtggcttcgg gctgcgtcat
     4141 tgattttgca ggcggcgcgg ctgaaccgcc gtgcctggcg gccgcgttcg acatcggcac
     4201 aaccacgctg gtgggggaac tcctcgattt gcgcacgggc gcgatatgcg ggtccgcctc
     4261 gcgcatgaat cctcagacca ttttcggcga cgacgtgctt tcgcggatca gccatgcgtc
     4321 acgcggcccg gaagcgttgc aggaagtcca taatacggtg gtatcggcgc tcaatgacat
     4381 gctcgctgag atgtcggcgc aggctgaggc atcggtggag aacatctacg agatctccgt
     4441 ttccggcaat accaccatgg agcatttttt tgcgggcttg aatccgggcg cgctgggcat
     4501 ggtgccgttc gtgcccgtgg cacgccgggg tttgaccctg gaccccgggc aggccggcgt
     4561 cgccatgaat cccagagggc gggtattcgt gctgccggtg atcggaggtt ttgttggcgg
     4621 cgacaccgtc gcgggcattg cggccacgcg ccttgaagaa tctgagcgcc cggcattact
     4681 catcgatatc gggacgaacg gcgagctggc gctcatccac aacggcggtt tgtcggctac
     4741 gtcgtgcgcg gccggtccgg ccttcgaagg ggcgcggatc tcctgcggca tgcgagcggc
     4801 cgccggagcg atcgaggccg ttgagctcgg cgacgatgtc cagagcaaaa ccatcggcgg
     4861 cgttcccccc atcggcttat gcgggtccgg cctgatcgat ctggtagcgg aactgctgcg
     4921 aaaaggcatt cttcagagcc agggcctctt taccccgcgc gatgcgctgc cggacgacct
     4981 gccacccgcc ttgcgcgacc gggtcgtgga ccatgcggaa ggggctgcct tcgtgttggc
     5041 gcgcgctgat gagacccgga tgcaacggcc gctcttgttc acgcaacggg atgtgcgtga
     5101 attgcaactg gccgcggggg ctatccgcgc ggggatttcc atcctcctca aacgggcgga
     5161 cctcgagccg gccgagctgg gtcatgtcta cgtggctggg ggattcggca actacatccg
     5221 gctggtcaac gcgcagcgca tcgggttgct gcctggagca ttgcgcgagg agcagttcag
     5281 ttttgcaggc aatacctcgc tcgaaggcgc ccgtgccgcc gcgctttctc tgggagcgag
     5341 agccagggcg gaagcgatct cttcgtccac ccggcacttt gatctctcgt gcgaccctga
     5401 attccagacc gagttcgcca tggccatgtt tttccccgat tcttcatcgg tcgagaccgt
     5461 ctgacggtcg ggatccgggc cggccgcgaa ttcgccgttg tgagtacgag gctgtatagt
     5521 ggtagcaggc atgcggatgt gcgatcataa gccagaagat gccgaaccca ggttcgtgtc
     5581 cgggaaaatg ttgaagggcc tgacttgtgg acacgcaatt cacagcgttg atgaagaatt
     5641 cctccgacgc ggtcttggtg atcgatgcga gttcgggcaa tgtggtggag tgcaacgaag
     5701 cagcgcttcg cctcctgaag accccggccg accgcatcct cggcaggcac cacacggccc
     5761 tgtatcctcc ggagatgcgc gaagattgcg cgcagcgctt gaagcagatc gccgcgggca
     5821 cgcccgctaa cgccgtagaa agtcaattgc agcagggaga cggcggaact gcctgggttg
     5881 aaatgagcgg cagcgcctgt gaggcgcatg gccgcatcct cgtgctgaat gccttccggg
     5941 atatgaccgg cttgcgccgc gcgaaagaag agaaccggcg cctgaaacgc attctgctgg
     6001 caatcaacgc atgcgacgaa gccatcatgg aggcgagcag cgagagccag cttctggccc
     6061 gggtcaccga atcgcttgtg gagaccgggg tatgcgatat tgccgccctg gtttgttttc
     6121 acagtgacgg tccggcaatc gccgcccaac acagccgcga cggcctggca gtgctggcag
     6181 ctgacctgcc gtcgctgtgc ggtgtgctgg gctggcccag ccgggggaag gcggcgcagc
     6241 ctgacatgaa ggttgcgcgc tttgagacag agggctcatc gccggctttt tcctgggccg
     6301 tggcatcggg cgaaagcccg ctgtgcctgg tgtcgttccc ctttgattgc gcgggacagg
     6361 agcaaggggc gctttttctg agttcgtttg cacacggggc attcgatcct caggaactcg
     6421 agatgctcgg cagagtgccg gatcacctgc ggaacgggat cgaaaccatc cgcacccgct
     6481 ccgagcgcga ccaggcactc gaagatctgc agtttcgcct gggcttggag gcgatcctgt
     6541 cccaggccat tcgcgaattc ttcaacctgg cagaacaagg ggttgacgcc tcaattcaag
     6601 cggcctgggg acgcatcagc gagtttctgg ggctcgacca cagcttctgc gtcatatttg
     6661 agaagggcga ttcgcccggg gcctcgtttg aatggcacgc cccggtactg gacagcctgc
     6721 caccggaggt ttcagcgctc gtactggcaa ccctctcccg gcacagttca cccgaattga
     6781 accgcggcga gcccctcatg atcaccgccg ggataccgga atttgccgat cggcgcgtgg
     6841 agcaggattt gtgcgaggtc ctggaagccc gctgcgtg
//
LOCUS       DAOPBQ010000147         6856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9870_length_6856_cov_3.792972, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000147 DAOPBQ010000000
VERSION     DAOPBQ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9870_length_6856_cov_3.792972"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PKW60_06240"
     CDS             <1..699
                     /locus_tag="PKW60_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99067.1"
                     /translation="DGHIRVLTGRAGNLPVGWLGEQLLLRQSGAAETGFAVSPPPYTT
                     TVPLALPEPPESGAFACEPGGGLCAFVSAAGGKVWVGKFPGEAFECVLEEGGATGRPC
                     ITSQGAVLVFTRRGAVHEFSGASGAWSRKTLLDLGHAVEKQRFSPDGTTVAWQEEARG
                     RLTASPLDSFAPAVVRLGESCVDFDWQDASTLVCLTRANEWQLPGRFHAVYTLQRIDM
                     STGSASAAARFPSS"
     gene            complement(714..1811)
                     /locus_tag="PKW60_06245"
     CDS             complement(714..1811)
                     /locus_tag="PKW60_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPJ99068.1"
                     /translation="MPSLQKILTYAVKHGASDVHLTVGSPPAIRLDGTIRFIGDDPIT
                     NDDNLQFIEEIMTPQDREEFMRTGDSDQALSFPGLGRFRVNILKQRNSVGIVMRHVKA
                     KIPSFEDLNLPPAIEKLTLMQRGLALVTGTTGSGKSTTLAAIIDKINADRRLHIVTIE
                     DPIEFLHSNKKSIVTQRELHIDTRDFHAALRAVMREDPDVILIGEMRDADTFQAAMAA
                     AETGHLVFSTLHTTNAMMTVDRIVDLFPSNQHTQIRSQLSLQLTACIAQRLIPSADGV
                     GRVPAVEVMFNNPAIAQLIKENNVRQIPNVIAGGREDGMQTFNMSLYDLIKKGLISED
                     QAMAASDNPEELSMNLQGITLSQGRGGIIRR"
     gene            complement(1997..2179)
                     /locus_tag="PKW60_06250"
     CDS             complement(1997..2179)
                     /locus_tag="PKW60_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99069.1"
                     /translation="MAGNASASPKKTGDRPPGQTATCAHCRYEYPVTEQRCVICGYPW
                     PWLETTQTPEAGPERR"
     gene            complement(2252..3469)
                     /locus_tag="PKW60_06255"
     CDS             complement(2252..3469)
                     /locus_tag="PKW60_06255"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPJ99070.1"
                     /translation="MATANSSSLALFGGTPVRDAGKPWPAWPIFDDQERQALMEVLNS
                     GKWWYGDRVAQFEREYAAFQDAGHCITVTTGTAAGEVLLQAIGLQPGDEVIVPPYTFY
                     ATASMVIRRGGIPIFVDIDDSWCMNPDLVEAAISPRTRAIMPVHLGGRICDMDRINEI
                     AAKHGILALEDACHSWGGKWKGKGTGALGKAGIFSFQASKNINAAEGGAIVTDDEELA
                     DLCRSITNCGRQKGGAWYSHPNLGTNARLTEFQGALLSVQLTRLEEHNRIREKNAAIL
                     DENLSKIEGLSTQLGDERITRRAYHLYCLRIDPEVFGCSREKVIEAAKAEGLPGISEG
                     YAVPLYEQPSIANAPNAPDYSKQECPVARDICTRSGIWIGHTTLLGSEEDMHDIIAIF
                     EKIKKNAEELDRA"
     gene            3769..4086
                     /gene="sugE"
                     /locus_tag="PKW60_06260"
     CDS             3769..4086
                     /gene="sugE"
                     /locus_tag="PKW60_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HPJ99071.1"
                     /translation="MPWICLLVAGVLEVVWAYAMKESHGFARLIPSIITISAMIASLW
                     LLAVAMRTIPLGTAYTIWTGIGAVGAFLVGIVFLSEPVNAARIIAAILIVSGLVLMKL
                     SSA"
     gene            4318..4449
                     /locus_tag="PKW60_06265"
     CDS             4318..4449
                     /locus_tag="PKW60_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99072.1"
                     /translation="MIFSTFFCWLESIIGGIFGFLNNILGGIFGIELPMPDFGCDRD"
     gene            4865..5803
                     /locus_tag="PKW60_06270"
     CDS             4865..5803
                     /locus_tag="PKW60_06270"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HPJ99073.1"
                     /translation="MGKGKLMEPGPIVYFEGRYVPVEEAKVSIMTHAFNYGTGLFEGI
                     RGYYSASEDNILVFRLAEHVERLVRNFNILCMEIPETPEDIATICVELIKRSGFREGV
                     YIRPLCYKSELSLGPRLSGVESRLCCYIIKLGEYCDIESGLDVVVSSWRRLSDNAIPS
                     RAKSTGSYINSSLAATEASLAGFDEAIFLREDGSVAEGSAMNLFMVMNGQLITTPPTA
                     DILVGITRNTVIELARNELGMDVVERPMSRTELYVCDEVFFCGTGAQVAPVRSVDRRV
                     IGGGTPGPISRALQDLYFQVVMGKVDKYRSWCTPVY"
     gene            5839..6504
                     /locus_tag="PKW60_06275"
     CDS             5839..6504
                     /locus_tag="PKW60_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPJ99074.1"
                     /translation="MHSEEALREAICETGRRLWQKGFVAASDGNISVRLSADTFLCTP
                     SQVSKGFMSPADLVTADASGRRLSGNGAVTSEFFTHLAAYEERPDVHAVVHAHPPKAV
                     AASLAGLSLAEPVLPELLLTIGGIPTVPYATPATGAGGEAIRGIIRSCDALIIARHGT
                     LTVGRDVFEAYHKLEKLEHAAEIILWASLLGPPAALPEAEVERLYRLQETYNPGTRLF
                     RPD"
     gene            6610..>6856
                     /locus_tag="PKW60_06280"
     CDS             6610..>6856
                     /locus_tag="PKW60_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99075.1"
                     /translation="MAGRASSNEDKTRVCPFCRREISIFATKCFHCGERVDPPRADQR
                     HFSMEDLGGRKSTNYAPSESVMAALETFRAELTGELQH"
BASE COUNT         1265 a         1984 c         2124 g         1483 t
ORIGIN      
        1 gatggacaca tccgcgtttt gacgggccgc gccgggaacc ttccagtcgg ctggctggga
       61 gagcaactgc tcctgcgcca gagcggcgcc gcggaaacag gatttgcggt atcgcctcca
      121 ccgtacacca cgaccgttcc gctggcgctg ccagaacctc ccgaatccgg cgcttttgcc
      181 tgtgaacctg gcggcggcct ctgcgcgttt gtctcggcag caggggggaa ggtctgggtt
      241 ggcaaattcc ctggagaggc gtttgaatgc gtgcttgaag aaggcggcgc aaccggcagg
      301 ccgtgcatta ccagtcaggg ggccgtgctt gtgttcacgc gccggggcgc tgtacatgaa
      361 ttttcaggag ccagtggcgc gtggagccgc aagaccctgt tggatctggg gcacgccgtt
      421 gaaaagcagc gattttcacc ggatggtaca accgtggcct ggcaggagga ggcacgcggc
      481 cgcctgacgg cgagcccgct ggattctttt gcgccggccg tggtccgtct gggcgaatcc
      541 tgtgtcgatt ttgactggca ggacgcaagc acgctggttt gcctcacccg ggcgaacgaa
      601 tggcagcttc cggggcgttt tcacgcggtc tacacgcttc agcgcatcga catgtctacg
      661 gggagcgcgt cggcagcagc ccgttttcct tcctcttgac ggaggcggga atctcagcgg
      721 cggataatgc caccccggcc ctggctgagg gtaatgccct gtagattcat ggacagttct
      781 tcggggttgt ccgaagcggc catcgcctga tcctcgctga taagcccctt cttgataaga
      841 tcataaaggc tcatgttgaa ggtctgcata ccatcttccc ggccgccggc gatcacgttg
      901 ggaatctggc gcacgttgtt ctctttgatg agctgggcaa tggccgggtt attgaacatg
      961 acctcaaccg ccggaacacg ccccaccccg tcggcactgg gaatcaggcg ctgggctatg
     1021 cacgccgtca gttgaagcga caactgggac cggatctgcg tgtgttgatt cgacgggaac
     1081 aaatccacga tgcggtcaac ggtcatcatg gcattcgttg tgtgcagggt gctgaagacc
     1141 aagtggccgg tttccgcggc ggccattgcc gcctggaagg tgtcggcgtc acgcatttca
     1201 ccgatcagaa tcacgtcagg atcttcacgc atgacggccc gaagggcagc atggaagtcg
     1261 cgcgtatcga tatggagttc ccgctgcgtc acaatgcttt tcttgtttga atgcagaaac
     1321 tcgatgggat cctcgatggt gacgatgtgg agccggcgat cggcgttgat cttatcgatg
     1381 atggcggcga gagtggttga tttaccgctt ccggtggtgc ccgtcaccag cgcgaggcca
     1441 cgctgcatca gggtgagctt ttcgatcgcg ggcggcagat tgaggtcttc aaagctcggg
     1501 attttggctt tgacgtgacg catgacgatg cccacggagt tgcgctgctt gaggatattg
     1561 acgcgaaaac ggcccaggcc tggaaaactg agcgcctggt cggaatcgcc ggtccgcatg
     1621 aactcctctc gatcctgggg cgtcatgatc tcttcaatga actgcagatt gtcgtcgttg
     1681 gtgatggggt catcgccgat gaagcgaatg gtgccgtcca ggcgtattgc ggggggactt
     1741 ccgaccgtca agtggacatc cgaagcgccg tgttttaccg catacgtcag gattttttga
     1801 agggaaggca ttcggttttc ctctctgcac ccgccgcccc ggcggggttg tctgcgctgg
     1861 cggccgggct ttcgaggccc gttgtccggc acgccgcaca cacgctctca cggaacctgg
     1921 ttcaagacgc aagccaagcc cggtgaatgt caaaactgta acagaaacca cgatttcagg
     1981 gcaaattccg ggcacgttat ctacgctcgg gccctgcctc gggcgtctgc gttgtctcaa
     2041 gccagggcca ggggtatccg cagatcacgc accgctgttc cgtcaccggg tattcataac
     2101 ggcaatgggc acaggtggcg gtctgtccgg gcggccggtc gccggtcttt ttgggggaag
     2161 cgctggcgtt gcctgccatg acactcctcc cgtccgcacc ctgttgtgca tcattccggc
     2221 ggcctgtttc ctggcgagcc attcgtccgg gtcaggcccg gtcgagctct tccgcattct
     2281 ttttgatctt ctcaaaaatg gcgatgatgt cgtgcatatc ttcttcggag ccgagcaggg
     2341 ttgtgtgccc gatccagatg ccgctccggg tgcagatgtc acgcgccacg gggcattcct
     2401 gcttcgaata gtccggcgca ttgggcgcat tggcgatcga aggctgctca tagagcggca
     2461 ccgcataccc ttcgctgatg ccgggcaacc cttccgcctt ggcggcttcg atgaccttct
     2521 cgcgggagca gccgaagacc tcggggtcga tgcgcaggca gtagaggtga taggcccgac
     2581 gcgtgattcg ctcgtctccg agctgagttg acaacccttc aatcttcgag aggttttcgt
     2641 cgagaatggc ggcatttttc tcgcggatcc ggttatgttc ttcaagccgc gtgagttgaa
     2701 cgctcaagag agcgccctga aactcggtga gccgggcgtt ggtaccgaga ttgggatgcg
     2761 aataccatgc accgcccttc tggcggccgc agttggtgat ggagcggcag agatccgcca
     2821 gctcctcatc gtctgtcacg atagcgccgc cttccgccgc gttgatgttc ttcgaggcct
     2881 gaaaactgaa gatgccggct ttgcccagcg ctccagtgcc ttttcccttc catttgccgc
     2941 cccaggaatg gcaggcgtct tcgagcgcaa ggatgccgtg tttcgctgcg atttcattga
     3001 tccggtccat gtcgcagatg cggccgccca gatggaccgg catgatcgcg cgggtccgtg
     3061 gcgaaatagc cgcctcgacc aggtcgggat tcatgcacca cgagtcatct atgtccacga
     3121 aaatggggat gccgccccgg cggatcacca tgctcgccgt cgcgtaaaac gtgtacggcg
     3181 gaacaatgac ctcatcaccg ggctgcaagc cgatggcctg cagcagaacc tcgcccgccg
     3241 cggtgccggt cgtcacggtg atgcagtgtc cggcatcctg aaatgcggca tattcacgct
     3301 cgaactgggc gacccggtcg ccataccacc atttgccgct attcagcacc tccatgagag
     3361 cctggcgctc ctggtcgtcg aagatgggcc aggcgggcca gggtttcccg gcatcgcgta
     3421 cgggagtgcc gccgaacagg gcaagtgatg atgaatttgc ggtcgccatg gtattcctcc
     3481 tacacaggtt ggctcgtcgt ggtatcacgt tgattttcct caatacctgc gactaagtca
     3541 aatgcccgcg ttcgacttcc cggcagctca gacggcagag cgctccaaca gaatagccag
     3601 ttgacctctt ctttccaggt gagccgtaat tccgtctctt catcggaaaa cgggattgat
     3661 cgattttcct tcgcgggtta gcatagcgcc gcctgaagga ggacgacctc ccccatccag
     3721 ggacgtaacg accaggacgg cctggccccc tgtattttgg ggagctcaat gccttggatc
     3781 tgtctgctcg tggccggtgt ccttgaagtg gtgtgggctt acgcaatgaa ggagtctcat
     3841 ggctttgcgc ggctcatacc gtcaatcatc acgatctccg caatgatagc cagtctctgg
     3901 ctcctggcag tcgccatgcg aacgattcca ctcgggacgg cctacacaat ctggactgga
     3961 ataggagcgg taggcgcttt tcttgttgga attgtgtttt tgtcggaacc ggtgaacgcc
     4021 gcaagaatca ttgcggcgat cctcatagtc tcagggctgg tgctgatgaa gctgtcaagc
     4081 gcctgacaca gtggcttcta ctgatggaac ttgcccctgg ccgtctcagc taatccgatt
     4141 gggaggcttg gaggctccac gccggccgga ggcgcaatgc gctttcatgc cggcacgctc
     4201 aatcccatcg tgacaccatc tggccgaaag cgccgggcgt gtaacatttg aggttgccac
     4261 ttgtaaggtt gagcgttccg gtaagtagtg tctaaccgtt cgaagaagga gagtaccatg
     4321 attttcagta cgtttttctg ctggctcgag agtatcatcg gcgggatttt cggattcctg
     4381 aacaacatcc tcggcgggat tttcgggatc gagctgccaa tgccggattt cggctgcgac
     4441 agggactagt ctcccggcgc attgaaggat acaccgggtt tttctttccg gcataacacg
     4501 gtagttgggc tcggtgagac gggcttatgg ctaagcttct ccaaaataaa ggagcttagc
     4561 catttttgtg tcaggccggt ttcaacgcct gggaatctgc cgctttgttc agtctggggc
     4621 aagttcatcc gcagtgcgtg ccggttgaga gctgcatttg catcgcgctg ctccaaagcg
     4681 cgatactgga ggccttcagg cgggcgccgg cccacgggcg atgcccgggg gcagttccgt
     4741 ttcgctgggc ttgcgtgcgt ctgaggcccg ggtgcagcag acagcggttt ttccaggctg
     4801 gccagccacg atccgggctg cggatttctg catgtatctc agaacgattt tcgggagaaa
     4861 ttcaatgggt aaaggtaagc tgatggaacc ggggccgatt gtctacttcg aaggccgcta
     4921 tgtaccggtc gaggaagcca aagtcagcat tatgacgcac gccttcaact acggcacagg
     4981 gctcttcgaa ggcattcgcg ggtattattc cgcaagcgaa gacaacatcc tggtttttcg
     5041 tttggcggag cacgtggagc gtctggtgcg gaacttcaac atcctgtgca tggaaatccc
     5101 ggaaacaccc gaagacattg ccactatctg cgtcgaactg attaaacgga gcggtttccg
     5161 tgagggcgtg tatatccgcc ccctgtgtta caagagcgaa ttatccctcg gtccgcgcct
     5221 gagcggcgtc gagagccggc tctgctgcta catcatcaaa ctcggcgaat attgcgacat
     5281 cgagtcgggt ctggatgttg tcgtctcatc gtggcggcgc ctgtccgaca acgccattcc
     5341 gagccgcgcc aagagcaccg gcagctacat caattcctcg cttgcggcca cggaggccag
     5401 cctggccggt tttgacgaag ccatttttct ccgggaagac gggagcgttg cggaaggcag
     5461 cgccatgaac ctgttcatgg tgatgaacgg ccagctcatt accacgccgc ccactgccga
     5521 tattctggtt gggatcacgc gcaacaccgt catcgaactg gcccggaacg aactgggaat
     5581 ggacgtggtg gaacggccca tgtcccgcac cgaactgtat gtatgcgatg aggtgttctt
     5641 ctgcggcacc ggcgcccaag ttgcgcccgt gcggtccgtc gatcgccgcg tgatcggagg
     5701 aggtacgccg gggccgatct cccgcgcact tcaggatctc tatttccagg ttgtgatggg
     5761 caaggtcgac aaataccgtt cctggtgcac gcccgtctac tgagcggacg gatccattcc
     5821 ggcgattaga gggcaggtat gcacagcgaa gaggcattgc gagaggccat ctgtgaaaca
     5881 ggccgccgcc tgtggcagaa gggctttgtt gccgcgagcg acgggaatat cagcgtccgc
     5941 ctctcagccg atactttcct gtgcactccg agccaggttt ctaaaggatt catgtcgccc
     6001 gcggatctgg tcacagccga tgcctccggc cgtagactat cggggaatgg cgcagtcaca
     6061 agtgagtttt ttacacacct ggccgcatac gaagagcgcc cggatgttca cgccgttgtg
     6121 cacgcccatc ctcccaaagc ggtggcggca tcgctcgcgg gtctgtcgct ggccgaacct
     6181 gtgctcccgg aactgctcct caccatcggg ggcatcccca ccgtgcccta cgccaccccc
     6241 gcaaccggag ccgggggaga ggcgattcgc ggcatcatcc gctcctgcga cgcgctgatc
     6301 atcgcgcggc acgggaccct cacggtgggc agggatgtgt tcgaggcgta tcacaagctc
     6361 gagaagctgg aacatgcggc ggagatcatt ctctgggcca gcctgctggg ccctcccgcc
     6421 gcgcttccgg aagcggaggt cgagagactg taccgcctgc aggaaacgta taatccgggc
     6481 acgcgcctgt tccgcccgga ttagcgagat gcaggcagct ggggcccgta gaaagcggct
     6541 tgtgtgtccc gcacccttgg gctatgctga gcgtgttgtg gacgcgtctt ctggcgatgg
     6601 agagacagcg tggctggacg agcttcgagc aatgaggaca agacccgtgt ttgccccttt
     6661 tgccgcaggg aaatcagcat cttcgcaacg aagtgtttcc actgtggcga acgcgtggac
     6721 ccgccccggg cggatcagcg ccacttttcc atggaggacc tgggcggccg caagtcgacg
     6781 aattatgcgc cctccgaaag cgtcatggcg gcgttggaaa cgttccgcgc cgagctgacc
     6841 ggcgagcttc agcacg
//
LOCUS       DAOPBQ010000148         6810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_9948_length_6810_cov_4.259660, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000148 DAOPBQ010000000
VERSION     DAOPBQ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9948_length_6810_cov_4.259660"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1077
                     /locus_tag="PKW60_06285"
     CDS             <1..>1077
                     /locus_tag="PKW60_06285"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003249779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPJ99076.1"
                     /translation="GSCSGMFTANSMNCLCEALGMALPGNGSLLATTAERRELVRAAG
                     RQIVELVKADIKPRDIITPKSIDNAFALDMAMGGSTNTVLHTLAIASEGDIEYPLQRL
                     NDVSARVPNICKVSPSNNVHMEDVDRAGGISAILAELAKKDGVLHLDCLTVTQKTLGE
                     NIAGAASKDPEIIRSLDNPYSDTGGLAVLFGNIAPDGAVIKAAGVAPEILKHTGTAIC
                     FDSEEDAMAGILEGKVKEGHVVVIRYEGPQGGPGMKEMLSPTSAIMGMGLGTKVSLIT
                     DGRFSGGTRGNCIGHVSPEAAAGGPIGLIQDGDTIEINIPGRTLNVKLDAAELEQRRK
                     ALGPPPDRKLSGWLQRYQRMVTSAN"
     assembly_gap    1078..1087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1088..2553
                     /locus_tag="PKW60_06290"
     CDS             <1088..2553
                     /locus_tag="PKW60_06290"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HPJ99077.1"
                     /translation="RIAVTFARTERPAAPTGFAGLMLQKNRGIVVMHTLTLRLLAVYA
                     VAASTLPNAASGDDGDSYGVKEIVDVAPVWSGHPVGFCLLTRGDQQYVAFYDASRRMT
                     VAQRMLGHAEWIQVHLPEQVHWDSHNSVTMAFDAAGCIHLSGNMHCAPLIYFKTREPG
                     DITTFERVTALVGSMEDKCTYPKFLTGPNGEFLFEYRHGSSGNGINLYTVYDTETQSW
                     RRLLDEPLLDGQGKMNAYANGPRLGPDGWYHLVWVWRNTPDCATNHDLSYARSRDLIH
                     WETSQGLALTLPITVDNGEIVDPVPPGGGIINGNTHVGFDGQNRVILSYHKYDAAGNT
                     QIYNARLEDGVWKFYQASDWTRRWEFGGKGAIPFDVHVSGVSVVDGTLMQSFSFSENG
                     GLWVLDPATLKPVGKAQHKPWGSVVAPPDARVPESDFPGMHVQWCPDSGETPGGEDWY
                     MLRWETLGANRDRPRDEPWPAPSFLRVYRMGRIPPTG"
     gene            2559..3539
                     /locus_tag="PKW60_06295"
     CDS             2559..3539
                     /locus_tag="PKW60_06295"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPJ99078.1"
                     /translation="MYVQVMGSGSWGLALARRLAMNGHHVRLWAREQDGPDMLRDERR
                     SPYFLPGILLPREIEVSREADPEVEAVVLAVPSHGMRAVLEAHPFSPATIRINAAKGI
                     ENTTLLRMDEVIHEVSGPCPVVTLSGPSHAEEVARDLPASLVAAGVDLGVCQTVQQVF
                     MADAFRVYTSTDIAGVGLGGALKNVIAIAAGVCDGFQLGDNAKAALITRGLAEITRLG
                     VAMGADPLTFAGLSGMGDLIVTCESRHSRNRGLGERIARGEAPEDFEKTSHMVAEGVR
                     TTQSAVALARKYGVDMPICGQVHRVLFEGGDPRQSIAALMQREPKPELRG"
     gene            complement(3580..4578)
                     /locus_tag="PKW60_06300"
     CDS             complement(3580..4578)
                     /locus_tag="PKW60_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPJ99079.1"
                     /translation="MEYRPLGNSELSVSAYAFGAWQLADTAYWGERDDADAEAVVQAA
                     IDAGVTLFDTAESYAGGRSEEVLGKALGARRSEVLIASKVSPDHCLRPETLRAACEAS
                     LKRLGTGVIDLYQVHWPCDPARFEDAAAELVRLKGEGKIRAIGVSNFGPRHLDAWMRS
                     GPCDSNQLGYNLLFRAIEYETAPACREHGVGILAYMPLMQGILAGRWKYVDEIPEMRR
                     RTRHFSSERPGTRHGEPGCETLLMRALEELEALCGELGEPMARVAIAWLLRQPGVASV
                     IVGGRTPEQLKRNLAAADLVLDDAIIQRLNKITGPLKESLGSNPDMWCGEAERRIH"
     gene            complement(4980..5498)
                     /locus_tag="PKW60_06305"
     CDS             complement(4980..5498)
                     /locus_tag="PKW60_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HPJ99080.1"
                     /translation="MSNVGPYLLDSDVFIQAKNQYYAFSICPGFWDCLLGHFQLGNIF
                     SLQEIREELLRGNDEEDLVRWVKNKLPRDFFLPASEQNVTRHFQDIILWVQENSQFKD
                     QAKSKFAAGADGWLAAFAKAHNYVVVTGEQFHPDAKNRVPLPNVCKKFDVQYGDVFKM
                     LCDLGVQFEWKR"
     gene            complement(5495..6652)
                     /locus_tag="PKW60_06310"
     CDS             complement(5495..6652)
                     /locus_tag="PKW60_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HPJ99081.1"
                     /translation="MNRVAVKPRLLNWALERSRKTPDELQKRQPCRRINKWLTGEVHP
                     THRQLQDFATATSTPFGYFFLPDPPEEALSIPDFRTLPRGAVRRPSANLIETLHTMQR
                     RRDWLRDVLIEESAEPLDFVGSASLSDPPENVGQEMRRAVGLDEGWAGEVRTWTEAVH
                     ELRRAIEKLGVMAVINGVVGNNTHRKLDVEEFRGFALSDAYAPLIFVNGADYRSAQMF
                     TLAHELAHLWLDSEGLSGFEGVIAGGSDVERFCDAAAAEFLVPGRELRAFWPSAGRDA
                     EPFQAIARQYKVSPIVAARRAWDLHLIGRSDFFGFYDDYRAEEHRKKVTGAGGGDFYN
                     NQNTRVGELFAAEVVRAAKEGRIQFREAYSLTGLYGAAFKNYAKHLGFDML"
BASE COUNT         1349 a         2200 c         1960 g         1291 t
ORIGIN      
        1 ggctcgtgct cgggcatgtt caccgccaat tcaatgaact gcctctgcga agccctgggc
       61 atggcgctgc ctggaaacgg ctcccttctc gccacgactg cggagcgccg cgaactcgtg
      121 cgggcggccg gccgccagat cgtcgaactc gtgaaggccg acatcaaacc gcgcgacatc
      181 attaccccga aatccatcga caacgccttc gcgctcgaca tggccatggg cggctcgacc
      241 aacaccgtgc tgcacacgct cgccatcgcg agtgagggcg atatcgagta cccgctgcaa
      301 cgcctcaacg acgtctcggc ccgtgtcccg aatatctgca aggtttcccc ttcgaacaat
      361 gtccacatgg aagacgtgga ccgcgcgggc ggcatctccg ccattctcgc agaacttgcg
      421 aaaaaagacg gcgttttgca cctggattgc cttacggtca cgcaaaagac gctgggcgag
      481 aatatcgcgg gcgcggcatc gaaagatccc gaaatcatcc gcagtctgga caatccctat
      541 tcggataccg gcggtctggc ggtactgttt ggcaacatcg cgccggacgg cgcggtgatc
      601 aaggcggccg gcgtggctcc cgagatcctc aagcacaccg gcaccgccat ctgcttcgac
      661 agcgaggaag atgcgatggc gggcatcctt gaaggcaagg tgaaggaagg ccatgtggtg
      721 gtcatccgct atgaaggccc ccagggcggc cccggcatga aggagatgct ctctccgaca
      781 tccgccatca tgggcatggg tctgggcacc aaagtgagcc tgattaccga cggccggttc
      841 tccggaggca cgcgcggcaa ctgcatcggc cacgtcagtc cggaagctgc cgccggcggg
      901 cccatcggcc tgatccagga cggcgacacc atcgaaatca acatccccgg ccgcactctc
      961 aatgtgaagc tcgacgccgc agaactggag cagcgccgca aggccctcgg gccacccccg
     1021 gaccgcaagc tcagcggctg gctgcagcgg taccagcgca tggttacaag cgccaacnnn
     1081 nnnnnnngcc gtattgcagt aacgttcgcg aggacggaac gcccggcggc gccaaccggt
     1141 ttcgccgggc tcatgttgca gaaaaaccgg gggatagtcg tcatgcacac cttaacgctt
     1201 cgactgctgg cggtgtacgc cgtggccgcc agcaccctcc cgaatgccgc ttccggagac
     1261 gacggcgatt cgtatggcgt gaaagagatc gtggacgtgg cgcccgtgtg gtcgggccat
     1321 cctgtcggtt tctgcctgct gacccgcggc gaccagcagt atgtggcgtt ttacgatgcc
     1381 agccgccgga tgactgtcgc ccagcggatg cttggccatg ccgaatggat ccaggtccat
     1441 cttcccgagc aggtgcactg ggacagccat aattcggtca ccatggcgtt cgacgccgca
     1501 ggctgcatcc acctctccgg caacatgcac tgcgcgcccc tgatctactt caaaacccgt
     1561 gagccgggcg acatcaccac ctttgagcgc gtcaccgcgc tcgtgggcag catggaggac
     1621 aaatgcacct accccaaatt tctgaccgga cccaacggcg agtttctctt tgaataccgg
     1681 cacggctcga gcggaaatgg aataaacctc tacacagtct acgatactga gacgcaatcc
     1741 tggcggcggc tgctcgacga acccctgctc gatggccagg gcaaaatgaa cgcctacgcc
     1801 aatggcccca ggctcggccc ggacgggtgg taccacctgg tctgggtgtg gcgcaacact
     1861 ccggattgcg ccaccaatca cgacttgtcg tacgcccgga gcagggacct catccactgg
     1921 gaaacgagcc agggcctggc gcttaccctt cccatcaccg tcgacaacgg cgaaatcgtt
     1981 gatccggttc ccccgggcgg cggcatcatc aacggcaaca cccacgtcgg attcgacggc
     2041 cagaaccgcg tcattctcag ttaccacaag tacgacgcgg cgggaaacac tcagatctac
     2101 aacgcccgtc ttgaagatgg cgtctggaaa ttctatcaag cctcagactg gacccggcgc
     2161 tgggaattcg gcggtaaggg cgccattccg tttgatgtac atgtcagcgg ggtatcggtc
     2221 gtcgatggca cgctgatgca atcgttcagt ttttccgaaa acggcggcct ctgggtgctc
     2281 gacccggcaa ccctcaaacc agtgggaaaa gcccagcaca agccctgggg ttccgtggtc
     2341 gcgccgccgg acgctcgcgt ccccgaaagt gactttccag ggatgcacgt ccagtggtgc
     2401 cccgattccg gcgagacgcc cggcggcgag gactggtata tgctgcggtg ggagacgctg
     2461 ggggccaatc gcgaccggcc gcgcgacgaa ccctggccgg ccccgagctt tttacgggtc
     2521 tatcgaatgg gccgcattcc accgacagga tgactgccat gtacgttcaa gtcatgggct
     2581 ctggaagctg gggactggca ttggcgcggc gcctggccat gaatggccat cacgtgcgcc
     2641 tctgggcccg cgaacaggac ggcccggaca tgctccggga cgagcggcgc agcccgtatt
     2701 ttctccctgg aatcctcctt ccccgggaaa ttgaggtgtc tcgggaggcc gatcccgaag
     2761 tcgaggccgt ggtgctcgcc gtaccctcgc atggcatgcg ggcggtgctc gaggcgcacc
     2821 cgttttcccc cgccacgatc cgcatcaacg ccgccaaagg cattgaaaat actaccctcc
     2881 tccgaatgga tgaggtcatc cacgaggtca gcggcccctg ccctgttgtg acgctgtccg
     2941 gtcccagcca cgccgaagaa gttgcacgcg atctgccggc ttcgctcgtc gccgccggcg
     3001 tcgatctcgg cgtgtgccag accgtgcagc aggttttcat ggcggacgcg ttccgcgtct
     3061 ataccagcac cgatattgcc ggtgtgggat tgggtggtgc gctcaaaaac gtgatcgcca
     3121 tcgctgcggg cgtgtgtgac gggttccagc tgggcgataa cgccaaagcg gcgctgatca
     3181 ctcgcgggct ggccgagatc acgcgcctgg gggtcgccat gggcgccgac ccgctcacgt
     3241 ttgcggggct cagcggcatg ggcgacctca tcgtgacctg cgaaagccgc cattcgcgca
     3301 accgcggcct gggcgaacgc atcgcccggg gcgaagctcc cgaagacttc gaaaaaactt
     3361 cgcacatggt tgccgaaggc gtgcgcacaa cccagtctgc cgtcgcgctt gcgcggaaat
     3421 acggcgtcga tatgcctatc tgcgggcagg tccatcgcgt gcttttcgaa ggcggcgacc
     3481 cgcggcagtc catcgcggcg ctcatgcagc gcgaacccaa acccgagttg cgcggctagg
     3541 ccaatcctcg gacagactca aaattgcccc cggtccggct cagtggatgc gccgctctgc
     3601 ctcgccgcac cacatgtccg gattggatcc gaggctttcc ttgagagggc cggtgatctt
     3661 gtttaaccgc tggatgattg catcatccag cacgagatcg gccgcggcga ggttccgttt
     3721 cagttgctca ggcgtgcggc cgccgacgat caccgaggcc acgcccggct ggcgcagcag
     3781 ccacgcgatg gccacgcgcg ccatgggttc gcccaactcc ccgcacagcg cctcgagctc
     3841 ctccagcgcc cgcatcagca gggtttcgca accgggctcc ccgtgccgcg tgccggggcg
     3901 ttccgaggag aaatggcgcg tgcggcggcg catttccggg atctcatcca catacttcca
     3961 gcggcccgcc aggatgccct gcatcagcgg catgtaggcc aggatcccga cgccgtgctc
     4021 ccggcatgcg ggcgccgtct cgtattcaat ggcccggaac aacaggttgt agccaagttg
     4081 gtttgaatcg cagggacccg atctcatcca tgcatccagg tggcgcggtc cgaaattcga
     4141 gaccccaatg gcgcggatct tgccttcgcc cttcagacgc actaattccg ccgccgcatc
     4201 ctcgaaacgt gcgggatcgc acggccagtg aacctgatac aagtcgataa cgccggtccc
     4261 cagccgtttc aggctcgcct cgcaggctgc acgcagggtt tcgggccgga gacaatgatc
     4321 cggcgagacc ttgctggcaa tgagcacctc gctgcgccgc gcgcccaatg ccttgccaag
     4381 cacctcctcc gacctgccac cggcgtagct ctccgcagta tcaaacagcg tgacgcccgc
     4441 gtctatggcc gcctgcacca ccgcctcggc gtccgcgtca tcgcgttcac cccagtatgc
     4501 cgtatcggcc agctgccacg cgccaaaggc ataggccgac acactcaatt cgctatttcc
     4561 aagcggacga tattccatcg gcagattctc cgtggtccgt aaattcacat attgacgaag
     4621 cttcagacgt catttcgggt aacaaacagt ggaaaagccc atatttcacg ccaatgagca
     4681 ggcaatccaa gcaagtcaaa gtgtatcgcg cctcgtttag gattccaaaa actgagccgg
     4741 cgcgcacttc ttacaacaaa cgtctagatc cacgccgggg cgcgcgaaag ggaaagcagg
     4801 aagccggtgg gactatctct gtgcacacgt agaccctggg ttcctgtcct gttgcctcat
     4861 tttggctgcc gcgcccggtt cgtcgcggca tttgggtcaa acgcccgccg ttttcccaca
     4921 aatatgactg attgtggtct taaagccgct ctcggcgggt tgctcgggca tgaccggcac
     4981 taccgcttcc actcaaattg cacgcccaaa tcacataaca ttttgaaaac atccccgtat
     5041 tggacatcga attttttgca cacatttggc agtgggactc tgtttttggc atcaggatga
     5101 aattgttcgc cagtaacgac aacataattg tgtgccttgg cgaatgcggc cagccagccg
     5161 tcggctcccg cggcaaactt gctctttgcc tgatctttaa actgtgagtt ctcctgcacc
     5221 cacaagatga tatcctgaaa gtggcgagtc acgttttgct cacttgccgg gagaaagaaa
     5281 tcacggggca atttgttctt cacccatcgc accaggtctt cctcgtcatt tccgcggagc
     5341 aactcttcgc gaatttcttg aaggctgaag atattcccca actgaaaatg cccgagaagg
     5401 cagtcccaga agcccggaca aatcgaaaag gcgtagtatt gattcttcgc ttgtatgaaa
     5461 acgtcggaat ccaggagata tggcccaacg ttgctcatag catgtcgaat cccaggtgct
     5521 tggcgtagtt tttaaacgca gccccgtaaa gaccagtcag gctgtacgcc tcccggaact
     5581 gtatccggcc ttcttttgcc gcccggacaa cctccgcggc aaacagctca ccgacgcgcg
     5641 tattctgatt gttgtagaaa tctccaccgc ccgcaccggt gacctttttc cgatgttcct
     5701 cagcccggta gtcgtcgtag aacccgaaga aatcgctccg accgatcagg tgcagatccc
     5761 aggctcggcg cgcagcgact atcgggctca ctttgtattg ccgggctatg gcctggaacg
     5821 gttcggcatc gcgccccgcg ctcggccaaa acgcccgtaa ctcgcgtccg ggaaccagga
     5881 actcagctgc tgcagcgtcg caaaaccgct ctacgtcgct gccgcccgca ataacaccct
     5941 caaaacccga gagtccctcg ctgtccagcc acaggtgggc cagttcgtgg gccagcgtaa
     6001 acatctgcgc cgatcggtaa tcggcgccgt ttacaaagat caatggagcg taggcgtcgc
     6061 tgagtgcaaa gccccggaat tcttctacat ccagcttgcg gtgcgtgtta tttcccacca
     6121 caccgttgat taccgccatc acacccagct tctcgatggc acggcgcagc tcgtgcacgg
     6181 cttcggtcca cgtgcgcact tcacccgccc agccctcgtc gaggcccact gcgcgacgca
     6241 tctcctgccc aacgttttcc ggaggatcac tgagactggc gctgcccacg aaatccaggg
     6301 gctcggcact ctcctcaatc aggacgtcgc gaagccaatc ccggcgccgc tgcatcgtat
     6361 gaagcgtttc aatcaggttt gcgctgggcc gccgcacagc ccctcgaggc aacgtccgga
     6421 aatccggaat ggaaagggcc tcttcggggg ggtcgggcaa gaaaaaatag ccgaagggtg
     6481 tcgacgtggc cgtggcgaaa tcctggagct gccgatgtgt cggatgcacc tctccagtaa
     6541 gccatttgtt gatacgccgg cagggttggc gcttctggag ttcgtcagga gtctttcgcg
     6601 atctttcgag cgcccagttg agcagtcttg gctttacggc tacgcgattc atcgactttg
     6661 ctgctctcca cctctcatta atagtatttt ctcacagaaa gatgcagcga tgcaaagaaa
     6721 gtgccggaat tcatgttccc tgcgccatgc ctttgcacga cagagtgagg ctgaactcct
     6781 tttcgccgtc gcgcgcacga ggcacaggca
//
LOCUS       DAOPBQ010000149         6730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10095_length_6730_cov_3.808390, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000149 DAOPBQ010000000
VERSION     DAOPBQ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10095_length_6730_cov_3.808390"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKW60_06315"
     CDS             <1..231
                     /locus_tag="PKW60_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPJ99082.1"
                     /translation="DPNHWTREELEPFFAHVFECFGFERTMFASDWPVMLIATTYPRW
                     VETVQWAIRGCSAAERRLLFRDTAEKYYRLGA"
     gene            complement(241..1071)
                     /locus_tag="PKW60_06320"
     CDS             complement(241..1071)
                     /locus_tag="PKW60_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPJ99083.1"
                     /translation="MHWKLLKQGLVYAAMLLVTALFVFPFYWLLISAFKTRAHIFVLP
                     PQFFPNPVVVQNFADVFRTTPIARAFVNSCIIAGGHTLLALFLCSLGGYAFAKFPGAP
                     GRDPLFAFVLGTMMIPAAVTLIPVFIVLTRLHLVNTYWAMILPGAANAFGIFWMRQYI
                     AANVHDELIAAARIDGCGEFGIYWRVALPIAKPALAALGILVLIGSWNNLMWAFIVLR
                     EKSMYTMPLLIYLLHGELRTPYGMVMAGGLLATLPLIAAFLVFQRSFIQGITAGALKA
                     "
     gene            complement(1076..1999)
                     /locus_tag="PKW60_06325"
     CDS             complement(1076..1999)
                     /locus_tag="PKW60_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPJ99084.1"
                     /translation="MAGAADSRGRFWGSLWRFRHFYLFISPFFFLFAVFGLYPLLFSL
                     YLSFVKWDGLTAPVSVGIENFITLFSDEMFYTALWNTLVIGLIYVPPMFVLAFLFASI
                     LNSKQLRLRGIFRAGVFMPCITPMVVVAIVFSLLYSAESGLFNYLLRGVTRLLPGGPV
                     DPIPWLESEAWSKLSVSILLVWRWTGYNMILMLAGLQGISGEYYEAAIIDGATRWQRM
                     RHITLPLMRPTLVFCGIMSLIGTVYMFDEVFVLTQGGPGTSSTNFGLYLFNLAFMDFR
                     FGFASSAAYTVAIAVFVVSLAVLKWRKPVVD"
     gene            complement(2001..3362)
                     /locus_tag="PKW60_06330"
     CDS             complement(2001..3362)
                     /locus_tag="PKW60_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPJ99085.1"
                     /translation="MKTERERGHELPAKRARGIRYRVPIALLFLSLAASDCLQGAVSP
                     APAGQRAAVEIWSWNIAAGSLMALAPEFERRHPGLEIRIDMTGAQMQSRFLLSLIAGV
                     GAPDISQLQLVDAPQFAPSGALMDLTDMAAPYASQFSPSFWENCLHEGRVYAIPWDMG
                     PCAVFYKRDLFARHGIDPESIETWADYVAAGKQMIKASGGQTRMLALSVSGLFPFFEI
                     LMQQTNGGIFDREGRIIIRSAENVAALEVLRAILEAGIAMPINTAGPEYYASFQNDTI
                     ATYPLAVWLGGSIKDYAAPTAGNWGVFRLPAVVPGGLRTSNLGGSVLVIPNQCQKKEA
                     AWKYVEYVLCTKEAQLAQYRNFDLFPCLMTTFDDPLFDEPDPFYGGQKVRRLFAQDIE
                     LIPTLTRTKDWNEAMRYLTQALSAWETNKLDHDLFLSQMAVKLKRRLGREYAPGHEPV
                     EIG"
     gene            complement(3382..4398)
                     /locus_tag="PKW60_06335"
     CDS             complement(3382..4398)
                     /locus_tag="PKW60_06335"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016537407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPJ99086.1"
                     /translation="MMRGRGFEKRHVNTVALLAALCIMVLGTHAAFSADTGAPLQGEA
                     GRQSDKIVIAHRGASGYLPEHTLAAYAMAYAQGADYIEQDLVLTKDAHFICLHDTDLG
                     GVTNVAEVFPDRKRENGKYYAADFTLAEIRQLRAESRDTRRFAPRLEGFRVATFVEAI
                     ELIQALNASTGRNVGIYPELKRPAWHREQGLPLEAAFMELVTRYGYTGPDARIYVQCF
                     EAPSLQLMRTEFGSQLPQILLTGGDADQPELQAEGLKQVATYAQGIGPSRGQIEKRPE
                     IVALAHAAGLVVHPYTFATDAVASQYPSFEAEVRHFYFEYDIDGMFTDFPDAARKALL
                     AGKP"
     gene            4505..6619
                     /locus_tag="PKW60_06340"
     CDS             4505..6619
                     /locus_tag="PKW60_06340"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPJ99087.1"
                     /translation="MHSTKMAPCILAACLTLFFHYTAAAAALPEGTALVEALGSEAAL
                     DHIEAKEASCRLGTASDASDAMLELAFEASTRPRIALRGPDGGWDWASVAGLAVRVTN
                     PGDSALTLEITAFGETADGHSRTMDGKATFEAGQTGWLRVYFSNNGRGPYWGMHGIPV
                     IGPVSRTMPGTASEARLTTASSITVAVREPEAAGRLLLGDLITFQEDSPVAQLVPMPF
                     VDKFGQFIHEDWPGKIHSEAELRAQLDREHAELEAAPALSGQDALGGWADGPQLAATG
                     WFRTEKLDGKWWLVTPEGRLFLSWGVNCLYPGDATFVTGREAWFEWLPEKDGPFAEFM
                     QSSQGAMMLADAIGNEGRTLRFYSMNLKRKFGETWEQEFRALALKRLRAWGFNTVANW
                     SLREVLEESTLPFTVNTGSHGGRMLEASSGYWGKMMDVFDPAFPVRVDDNIRRAAAPY
                     AANPLVVGYFVDNEMSWVSIAASTLSCPPDQPARVVFIDRLKAQYETLDALNAAWGTD
                     AESWEALRLPGRQTEAGKADAEAFEYAFAHHYFSTIAGAIRKYAPNQLYLGCRFTPFY
                     CPKPVLQACADVVDVVSINFYLPSVPPAVMSGIDKPVVIGEFHFGALDRGMFHTGLCA
                     AASQDERGELYAQYVKSVAEHPNFVGCHWFQYVDQPLTGRNMDGENYNIGLVSGVDVP
                     YEPFLSYVREAHRAMYAHRQAH"
BASE COUNT         1355 a         2085 c         2072 g         1218 t
ORIGIN      
        1 gacccgaatc actggacccg ggaggagctt gagccgtttt ttgcccacgt gttcgaatgt
       61 ttcggctttg aacgcactat gtttgcgagc gactggccgg taatgctgat tgccacgacc
      121 tatccgcgct gggtcgagac cgttcaatgg gccatccggg gctgctccgc cgcggagcgc
      181 cggctgctgt tccgtgatac cgcggagaaa tattaccggc tgggcgcctg accgggggac
      241 tcaggctttc agagcgcccg cggtaatgcc ctggatgaat gatcgctgaa acacgagaaa
      301 ggcggcgatc agcgggagcg tcgcaagcag accgcccgcc atgaccatgc cgtacggtgt
      361 gcggagttca ccatgcagca gatagatgag caggggcatg gtgtacatgc tcttttcccg
      421 caacacgatg aacgcccaca tcaggttgtt ccacgagccg atcagcacga gaatgcccag
      481 tgccgccagc gcgggtttgg caatggggag ggccacccgc cagtagatgc cgaattcacc
      541 gcagccgtct atgcgcgccg cggcgatcag ctcgtcatga acgtttgctg cgatgtactg
      601 ccgcatccag aaaatgccga aggcgttggc cgcgccgggg aggatcatcg cccagtatgt
      661 gttcacgaga tgcagccgcg tgagcacgat gaagaccggg atgagggtga ccgccgcggg
      721 gatcatcatc gtgcccagca cgaatgcaaa aagtgggtct cgcccgggcg cgccgggaaa
      781 tttcgcgaat gcatatccgc ccagggagca tagaaacagc gccagaagcg tgtggccgcc
      841 cgcgatgatg cacgagttga cgaaagcccg ggcgatgggc gtggtccgga aaacgtccgc
      901 gaaattctgc acgaccaccg ggttcgggaa aaattgcgga ggcagcacga agatgtgcgc
      961 ccgggtcttg aacgccgaga tgagaagcca gtagaaaggg aacacgaaga gcgccgtgac
     1021 cagtagcatc gccgcataga cgagcccctg tttcagcagc ttccagtgca tcgcgttaat
     1081 ccaccacagg tttgcgccac ttcaacaccg ccagggacac cacaaaaact gcaatcgcta
     1141 ccgtgtaggc cgcgctggac gcgaaaccga aacggaaatc catgaaggca agattgaaca
     1201 ggtagagccc gaaattggtt gatgacgtcc ccggaccgcc ctgcgtcagg acgaatacct
     1261 cgtcgaacat gtagaccgtg ccgatgagcg acatgatccc gcagaagacc agcgtggggc
     1321 gcatcagcgg cagggtgata tggcgcatgc gctgccaccg ggtcgctccg tcgatgatcg
     1381 ccgcttcgta gtactcaccg gaaatgccct gcagcccggc gagcatcagg atcatgttgt
     1441 agccggtcca gcgccatacg aggaggatcg ataccgagag tttcgaccag gcttcgcttt
     1501 cgagccaggg gatcggatcc accgggccgc cggggagcaa gcgggttacc ccgcgcaaca
     1561 ggtaattgaa caggcccgat tcagcgctgt acagcaggct gaagacgatc gcgaccacta
     1621 ccatcggcgt gatgcacggc atgaacacac cggcgcggaa tattccccgc aaacgaagct
     1681 gcttcgagtt gagaatgctc gcgaaaagaa atgccagcac aaacatgggc ggcacgtaga
     1741 tcagcccgat gaccagcgtg ttccacaggg ccgtgtagaa catttcgtca gaaaacagcg
     1801 taatgaaatt ctcgatcccg acggataccg gcgccgtgag cccgtcccat ttcacgaagc
     1861 tcaaatacag cgaaaacagg aggggataga ggccaaacac cgcaaaaaga aagaagaacg
     1921 gcgagatgaa caggtaaaaa tggcggaaac gccagagcga gccccagaac ctcccccggc
     1981 tatccgctgc ccccgccatg tcatccgatc tccacgggtt catggccagg agcatactca
     2041 cggcccagcc ggcgtttgag tttcacggcc atctgcgaca gaaacaggtc gtggtcgagc
     2101 ttgtttgtct cccacgcgct caacgcttgc gtgaggtatc gcatggcttc gttccagtct
     2161 ttcgtgcgcg tgagcgtcgg gatcagctca atgtcctgag cgaacaggcg ccgcactttc
     2221 tgccctccgt agaacggatc aggttcgtca aagaggggat cgtcaaaggt ggtcatcagg
     2281 cacggaaaca ggtcaaaatt gcggtactgc gcgagctggg cttccttcgt gcacaggacg
     2341 tattccacgt atttccacgc cgcctccttc ttctggcact gattgggaat caccagcacc
     2401 gatccgccca ggttgctggt gcgcaggccg ccgggcacga cggccggcag ccggaacacg
     2461 ccccagttcc ccgcggttgg cgcagcgtaa tctttgatcg agccgcccag ccagactgcc
     2521 agggggtagg tggcaatcgt gtcattctga aacgaggcat aatattccgg accggccgtg
     2581 ttaatcggca tcgcgatgcc cgcttcgaga atcgcccgca aaacctccag cgcggccacg
     2641 ttctccgcgg aacggatgat gatccgccct tcccggtcga aaataccgcc attcgtctgc
     2701 tgcatcagga tttcgaaaaa cgggaacagc cccgataccg agagggccag catacgggtt
     2761 tgtccacccg aggcttttat catctgcttg ccggcggcga cataatcggc ccaggtttcg
     2821 atggattccg ggtcgatgcc gtgccgtgca aacagatcgc gcttgtaaaa caccgcgcag
     2881 ggccccatgt cccacggaat ggcatagacg cggccttcgt gcagacagtt ttcccagaaa
     2941 gagggcgaaa attggcttgc atacggcgcc gccatgtccg tcaggtccat gagcgccccg
     3001 ctgggggcga attgaggcgc gtcgaccagt tgcagctggg agatatccgg tgcgcccacc
     3061 cccgcgatca acgacagcaa aaaacgggac tgcatctggg cgccggtcat gtcgatgcgg
     3121 atctcgagcc cgggatgccg ccgctcaaat tcgggcgcga gagccatcag gctgcctgcg
     3181 gcgatgttcc agctccagat ctcaacggcc gcgcgctggc cggccggtgc cggggaaacc
     3241 gcgccctgaa ggcagtcact cgccgccagc gacaggaaaa gcagcgcaat ggggacacga
     3301 tacctgatgc cacgcgcccg cttggctgga agctcgtgtc ccctctcgcg ctcagttttc
     3361 actcagtgca gggtctccga atcagggttt cccggcgagt agcgctttgc gcgctgcgtc
     3421 cggaaaatcg gtaaacatgc cgtcaatatc atactcgaaa tagaaatgcc gcacttcggc
     3481 ttcaaacgac ggatattgcg acgcgacggc atcagtggcg aatgtgtagg gatgcaccac
     3541 caacccggcg gcatgggcga gcgcaacgat ttccgggcgc ttttcgatct ggccgcggga
     3601 cggcccgatc ccctgggcat acgtcgcgac ctgcttcaac ccttccgcct gcagctcagg
     3661 ctgatctgca tcccctccgg tgagcaggat ctgcggcagc tgcgagccga attcggtacg
     3721 catgagctgc agcgatggcg cttcgaagca ttgcacgtag atgcgcgcat ccgggccggt
     3781 gtaaccgtag cgcgtcacca gttccatgaa cgccgcctcc aggggcaatc cctgctcccg
     3841 gtgccaggcc ggccgcttca gctcggggta gatacccaca ttgcgcccgg tcgacgcatt
     3901 gagcgcctga atgagctcaa tggcttcgac gaacgtggcc acgcggaatc cctccagccg
     3961 aggtgcgaag cgccgggtgt ctctggattc ggcgcgcaac tggcggatct ccgccagggt
     4021 aaaatccgct gcgtaatact tgccgttctc gcgctttcga tccgggaaca cctccgccac
     4081 gttggtgacg cctcccaggt cggtgtcgtg caggcaaatg aaatgggcgt ctttcgtgag
     4141 caccaggtcc tgctcgatat aatctgcccc ctgcgcatac gccatggcgt aggcggcgag
     4201 ggtatgttcg ggcaggtagc cactggcgcc gcggtgggcg atcacgattt tatccgattg
     4261 ccggccggcc tctccctgca gcggcgcgcc agtatccgcc gaaaacgctg cgtgcgttcc
     4321 caggaccatg atgcagagcg ctgccaggag cgccaccgtg tttacatgcc gtttctcgaa
     4381 tcctcgtcct ctcatcatac tctccttgtt gaactgcaaa cttgtcttgg caatactacg
     4441 gcgaatggca tcgtttgggc aattcccgca acgcacagcc tgcaatttcc ggatggaggc
     4501 gcatatgcac agcacgaaaa tggcgccatg tatcctggcg gcatgcctga ccctgttctt
     4561 ccactacaca gctgcagcag cggcgcttcc cgagggtacg gcccttgtcg aggcgctcgg
     4621 ttccgaggcg gcgctcgacc acatcgaagc caaagaagca tcctgccggc tgggtaccgc
     4681 gtccgatgct tcggacgcca tgcttgagct ggccttcgaa gcttcgacgc gcccgcgcat
     4741 cgcgttgcgg ggaccggacg gaggctggga ctgggcgtcc gtggcgggcc ttgcggtccg
     4801 ggtcaccaac ccgggagatt cggcgctgac gctggagatc acggccttcg gagagactgc
     4861 tgacggccat tcgcgcacca tggacggcaa agccactttt gaagccggcc aaaccggctg
     4921 gctccgcgtg tatttcagca acaacggccg cggtccgtat tggggcatgc acggcatccc
     4981 ggtgattggc ccggtttcac gcacgatgcc cggcacagcg agcgaggccc ggctgacgac
     5041 ggcatccagc atcaccgtgg ccgtgcggga gcctgaggcg gccgggcggc tgcttctggg
     5101 cgacctgatc accttccagg aggacagtcc cgtcgcgcag cttgtgccta tgccgtttgt
     5161 ggataaattc gggcaattta ttcatgagga ctggccgggc aagatacatt ccgaggcgga
     5221 gctgcgggcg cagcttgacc gggaacacgc cgagctggag gcggcgccgg cactgtccgg
     5281 ccaggacgcg ctgggcggct gggccgacgg gccgcagctg gccgccaccg gctggttccg
     5341 caccgaaaaa ctcgacggca agtggtggct ggtcacgccc gaggggcgcc tgtttctctc
     5401 ctggggcgtg aattgcctct atccgggcga cgccaccttt gtgacgggcc gggaggcctg
     5461 gttcgaatgg cttcctgaaa aagacggccc cttcgcggaa ttcatgcaaa gcagccaggg
     5521 cgccatgatg ctggccgacg ccatcggcaa cgaaggccgg acgctgcgct tctattccat
     5581 gaatctgaag cgcaaatttg gcgagacctg ggaacaggaa ttccgcgcgc tcgccctgaa
     5641 acggctccgg gcctgggggt tcaacaccgt cgcaaactgg tccctgcggg aggtcctcga
     5701 agagagtact ctgcccttta ccgtgaacac aggctcgcac ggcggccgca tgctcgaggc
     5761 aagctccggg tactggggca aaatgatgga cgtgttcgat ccagcattcc cggtgcgcgt
     5821 ggatgacaac atccgccgcg ccgccgcacc ctatgccgcc aatccgctgg tggtgggata
     5881 cttcgtggac aatgagatgt cctgggtgtc catcgccgcc tcgaccctga gctgtccgcc
     5941 cgaccagccc gcgcgggtcg tcttcattga ccggctgaaa gcgcaatatg agacgctcga
     6001 cgctctcaat gccgcctggg gtaccgacgc cgagtcgtgg gaagccctgc gcctgcccgg
     6061 ccggcagacg gaggcgggca aggctgatgc cgaggccttc gaatatgcct tcgcgcacca
     6121 ctatttctcg accattgccg gggccatccg caaatatgcg ccgaatcagc tttacctggg
     6181 ctgccgtttc accccgttct actgcccgaa acccgtcctg caggcctgcg ccgacgtggt
     6241 cgatgtcgtc agtatcaact tctatctccc gtcggtgccg cccgccgtga tgagcggcat
     6301 cgataaaccg gtggtgatcg gagaattcca ttttggcgcg ctcgatcggg gcatgtttca
     6361 taccggcctc tgtgccgcgg ccagccagga tgagcgcggc gaactctacg cccaatacgt
     6421 caaaagcgtc gctgaacacc cgaactttgt cggatgccat tggtttcagt acgtcgacca
     6481 gcctttgacc gggcgcaata tggacggcga gaactacaac atcggcctcg tgagcggggt
     6541 cgacgtgccc tacgagccgt tcctcagcta tgtccgggag gcccaccgcg ccatgtacgc
     6601 ccacaggcag gcgcattgat ctttgcggaa gcccgaattt ccgccgggct cggcccggtc
     6661 agcgacggtc agcctgcgtt acatgattgc atcgcacctt cgcggaggtt atcataaatt
     6721 ggcttatccg
//
LOCUS       DAOPBQ010000150         6726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10102_length_6726_cov_4.647129, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000150 DAOPBQ010000000
VERSION     DAOPBQ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10102_length_6726_cov_4.647129"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PKW60_06345"
     CDS             complement(<1..864)
                     /locus_tag="PKW60_06345"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HPJ99088.1"
                     /translation="MDPLMIVWVAVAVVIGILLSAIGSRIMGRRYMSTARREAAQAIS
                     DAEREAATILKEAQTELKEQRIELRAEVEREAREQRRELVALEKRILAKEESVDKRVE
                     LLETKANEVSTKDRELAQREQALENRQREIDQIIQEQRTKLENVSGMSAEQAKRELFT
                     QLENEVRRDTALRLKRIEDELVENSEKRAQWVISQAIQRCAADHVAESTVSVVDLPND
                     EMKGRIIGREGRNIRALESVTGINIIIDDTPEAVILSGFDPIRREVARIALERLIQDG
                     RIHPARIEEVVA"
     gene            1341..2915
                     /locus_tag="PKW60_06350"
     CDS             1341..2915
                     /locus_tag="PKW60_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPJ99089.1"
                     /translation="MVSRREFLGRASCCALAALTAARPRPARAAADVQRPNIVWIYSD
                     DHAQNAVSAYGSRLASAAPTPNIDRIAREGVIFRNSFVTNSLCGPCRAVVLTGKHSHI
                     NGFCTNSDEFDGSQQTFPRLLQNAGYQTALFGKWHLVSEPTGFDAWEVLPGQGVYYNP
                     DFITAQGRHRRDGYVTDIITDLALDWLQNERNAEKPFMLMIQNKAPHREWEPAPEHLT
                     TYDDVTIPEPDNLFDDYSGRGTAAREQDMTIAETMRLGPNLKVWETVPDDDRDKQRTF
                     GRMAGEQRERWQAAYGPKNKAFLEANLQGDDLVRWKYQRYMKDYLRCIASIDDNVGRV
                     LDYLEASGLDKNTVVFYASDQSFYLGEHGWFDKRFIYEESLRTPFVARWPGVTPPGSE
                     VEHMVQNLDCAQTFLDIAGVPIPGDMQGRSLRPFLLGQPPQDWRTSIYYHYYEGEGKV
                     HNVYKHYGVRTARYKLVHFYTLGEWEFYDLENDPHEMRSEYANPDYAPQIAELKAELA
                     RLRELYRVPEEPAAQT"
     gene            complement(2918..4306)
                     /locus_tag="PKW60_06355"
     CDS             complement(2918..4306)
                     /locus_tag="PKW60_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HPJ99090.1"
                     /translation="MSTVTERWVCTVCGYVHEGPEPPEACPVCGAGPDMFEAEEEPKT
                     SAPAAPDTTASEEYLAKWARASDGFETKFARIKALASGAPSEISPMRTQQPFPSWESV
                     LFRGAQLARLPRNEDEPVNTRTVLGPAAEKPLTIAMPFYVSHMSFGALSREAKIALAR
                     GSRLAGTLICSGEGGLLPEERAEASLYIYELGTATFSHRDDAIRQADAVEIKIGQAAK
                     PGLGGQLPAEKVTEEIARVRGIPAGHDSISPGRFSGINEKGDLKKVVERVRGLLDGKP
                     LGIKLTASHIEDDLAEALEASPDFITIDCRGGATGAAPTYIKDNVCLPPVFAVYRARK
                     FLDSVGSRVTLCVTGGFRDSADIAKALALGADAVALATASLIAIGCQQYRICHTGRCP
                     VGITTQDPELRKRLDVEQSAQRLANFYATTNLEVANFARINGRDDVHALDLSDLVTIS
                     NEISQNTGIEHA"
     gene            complement(4388..4888)
                     /locus_tag="PKW60_06360"
     CDS             complement(4388..4888)
                     /locus_tag="PKW60_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPJ99091.1"
                     /translation="MSTADNLQEAFAGESQANRKYLAFATKAEKDGFPQVAKLFRAAA
                     EAETIHAHAHLRVMGGIGTTAENLKAGIAGEGHEFKTMYPEFVAAAQEEGNKPAEITF
                     RNAMAVEEIHHGLYQEALAAVEAGNDLAAAAIYVCPVCGNTVIGGVPDKCEICGVPGS
                     RFFEIH"
     gene            5511..6323
                     /locus_tag="PKW60_06365"
     CDS             5511..6323
                     /locus_tag="PKW60_06365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPJ99092.1"
                     /translation="MHDLKRYGTWALITGASAGIGKAFAEAIAAQGLNCVLVARRLDR
                     LQDLAADLQSRHGVECRCVNEDLAQPGSAYRVAEAVADLEVGLLINNAGVGYAWPFED
                     SDPERLADLITLNCVTPALLTRLLAPEMIKRRRGGIILVGSVLGVIPSPFHTVYGASK
                     AFAVALAEGLYGEYEQAGIDVLALCPSTTRTEFFVAEGMSEARANRLMAGADRPEAIA
                     ELGLRYLGRKPRVGPWAYTGPALLARLLPRKWSIRLVRKFMRPYLQKDEKSG"
     gene            complement(6351..>6726)
                     /locus_tag="PKW60_06370"
     CDS             complement(6351..>6726)
                     /locus_tag="PKW60_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPJ99093.1"
                     /translation="EITANPINNSSIAFRLWDTAKSVLFTGDLGVEGGDKLLNSDQAR
                     HLPADYIQMAHHGQRGVSEAFYTAVNPSFCLWPTPKWLWDNDNGGGPDSGPWKTLEVR
                     AWMNKLTIKRHYVMHEGLHEIR"
BASE COUNT         1221 a         2099 c         2090 g         1316 t
ORIGIN      
        1 ggcgaccacc tcttcgatgc gggccgggtg gatgcgtcca tcctgaatca ggcgttcgag
       61 cgcgatacgc gccacctcgc gccggatcgg gtcaaacccc gacaggatga cggcctcggg
      121 cgtatcgtcg atgatgatgt tgatgcccgt gacgctttcc agggcgcgga tattacggcc
      181 ctcgcgcccg ataatgcgcc ccttcatctc gtcgttgggc agatcaacca ccgacaccgt
      241 gctttcggca acgtggtctg cagcgcagcg ctgaatcgcc tggctgatga cccattgggc
      301 gcgtttttcg gaattttcga cgagttcgtc ctcgatccgt ttgagccgca gggcggtgtc
      361 gcgccggact tcattctcca gctgagtgaa cagctcacgc ttcgcctgtt ccgcactcat
      421 ccccgataca ttttcgagtt tcgtgcgttg ctcctggata atttgatcga tctcgcgctg
      481 acggttctcg agcgcctgct cgcgctgcgc cagttcgcga tctttggtgc tgacttcatt
      541 ggctttggtc tcgagcagtt cgacgcgctt gtcaacgctt tcctctttgg cgaggatgcg
      601 cttttccagc gcgacgagct cgcggcgctg ttcgcgggcc tcgcgttcga cttcggcccg
      661 cagttcgatt cgctgttctt tgagttcggt ctgtgcttct ttgaggatcg tcgcggcttc
      721 acgttccgca tcggagatgg cctgggcggc ctcgcggcgc gccgtgctca tgtaacggcg
      781 gcccataata cggctgccga ttgcactgag gaggatcccg atgacgacgg ccactgcgac
      841 ccacacgatc atgagtggat ccatgtgtga acctccgccc cctgggggac agttaaatcc
      901 gacgggagcg taaggcgaaa gaaggcatgc ggggttgggt cagcccaact ccagaaggtt
      961 gcaccattta gctttgagaa aactcacatc tgcgatcaaa ttagcccacc catcatatct
     1021 gaacaccgtc acgacagtag gccgcggccc cccctctgct gaagccgccg agcctcgcgc
     1081 tgtctcgcaa cgtgggcaaa atgcaaccaa tatgctctac cgccccactt ccaatcggac
     1141 aagctaaggt caaaacgtta cacaatactg actaaagacg gtttaagtat atttccagca
     1201 cccgcctaag tcaaacaaac attctcacgg cgccccgcga gaccgcgggt tcagaattcc
     1261 agcgcgggat ttgtcaatca ctttccgtct gtgccaagat ggcaattgct gccgtaagcc
     1321 gactcatgga gaggaagatc atggtttcaa gacgcgaatt tctcgggcgc gcgagctgct
     1381 gcgcactggc tgccctgaca gcggcccgac cccgtccggc gcgggcggct gcggatgtcc
     1441 aacggcccaa tattgtatgg atttacagcg atgaccacgc ccagaacgcc gtcagcgcat
     1501 acggcagccg actagcttcg gcagccccta ctccgaacat cgaccggatc gcgcgcgagg
     1561 gcgtcatttt ccgcaatagc ttcgtaacca actcgctgtg cggaccgtgc cgggcggtcg
     1621 tccttactgg taaacacagt catatcaacg gattctgcac aaacagcgac gaatttgacg
     1681 gttcccagca gacattcccg agactcctcc agaacgcggg gtaccagacc gccctctttg
     1741 gaaaatggca tctcgtcagc gagcccacgg gctttgacgc ctgggaagtg ctgccgggtc
     1801 agggcgtcta ctacaatccc gatttcatca cagctcaggg gcgccatcgc cgggacggat
     1861 acgtgaccga catcatcacc gatctggctc tggactggct ccaaaatgaa cgaaatgcag
     1921 aaaaaccatt tatgctcatg atccagaaca aagccccgca ccgggaatgg gagccggcgc
     1981 cggaacacct taccacctac gacgacgtca ccattcccga accggacaac ctcttcgacg
     2041 attactccgg gcgaggcacg gcagcccggg aacaggacat gaccatcgcg gagaccatgc
     2101 ggctgggacc gaacctcaag gtctgggaaa cggtcccgga cgatgaccgc gacaagcaac
     2161 ggaccttcgg ccgcatggcc ggcgaacagc gcgagcgctg gcaggcggcc tacggcccga
     2221 aaaacaaggc gtttctggaa gcaaatctgc aaggtgacga cctggtgcga tggaaatacc
     2281 agcggtatat gaaggattac ctccggtgca tcgcatctat cgacgacaat gtgggccgcg
     2341 tgctcgacta tctggaagcc tccgggctcg acaaaaacac tgtggtcttc tatgcgtccg
     2401 accagagttt ttatctgggc gagcacggct ggttcgacaa gcggttcatc tacgaggaat
     2461 ccctgcgcac cccgtttgtt gcccgctggc ccggggttac cccgcccggc agtgaggtcg
     2521 agcatatggt gcagaacctc gattgtgcgc agaccttcct ggacatcgcc ggagtcccca
     2581 tacccggcga tatgcaaggc cgcagccttc ggcccttctt gcttggccag ccgccccagg
     2641 actggcgcac atccatctac taccactact atgaaggcga ggggaaggtc cataatgtgt
     2701 acaaacatta tggcgtgcgg accgcgaggt acaagctggt ccacttctac acgctgggcg
     2761 agtgggagtt ctacgacctc gaaaacgatc cgcacgagat gcgcagcgaa tacgcgaatc
     2821 ccgattacgc gccccagatc gcggaattga aggcggaact cgcccgcctg cgcgaactct
     2881 accgcgtccc cgaagaaccg gcggcacaga cgtaggctca ggcgtgctcg atccccgtat
     2941 tctgggaaat ctcgttgctg atggtcacca ggtcggacag atcgagggcg tgaacgtcgt
     3001 cgcggccgtt gatgcgggcg aaattggcca cttccaggtt ggtggtggca tagaaattgg
     3061 ccagccgctg ggccgattgc tcgacgtcaa gccgtttccg cagctcggga tcctgtgtgg
     3121 tgatgccgac ggggcagcgc ccggtatggc agatgcggta ttgctggcag ccgatcgcaa
     3181 tgagtgacgc ggtggcgagc gccacggcat cggcgcccag ggcaagggct ttggcgatgt
     3241 cggcgctgtc gcggaatcct cccgttacac agagggtgac acggctgccc acgctatcga
     3301 ggaacttgcg ggcgcgatac acggcgaaca cgggcggcag gcacacattg tccttgatat
     3361 acgtgggcgc ggcgccggtc gcgccgccgc ggcagtcgat ggtgatgaaa tcggggcttg
     3421 cctccagggc ctcggcgaga tcgtcttcaa tatggctggc agtcagcttg atacccagcg
     3481 gcttgccgtc gagcagtccg cgcactcgtt ccaccacctt tttcagatcg cccttctcgt
     3541 tgatccccga aaaacggccg ggagaaatcg agtcgtgccc cgccgggatg ccacggacgc
     3601 gcgcgatctc ctcggtcacc ttctccgcag gcagctgtcc gcccagtccg ggtttggcgg
     3661 cctgcccgat cttgatctca acggcgtcgg cctggcggat ggcgtcgtcg cggtgcgaaa
     3721 aagtcgccgt gccgagctcg tagatgtaga gcgaagcctc ggcgcgctcc tccggcagga
     3781 gaccgccttc gccggagcag atgagggtgc cggccaggcg cgagccgcgg gccagggcga
     3841 tcttcgcttc gcgtgacagc gccccgaaag acatgtgcga cacatagaac ggcatggcga
     3901 tggttaacgg cttttcggcg gcgggtccga gcaccgtgcg ggtgttgacc ggctcgtctt
     3961 cgttgcgggg caggcgcgcc agctgcgcac cgcgaaacag cacggactcc cacgacggaa
     4021 acggttgctg ggtccgcatg ggcgagatct cgctgggagc gcccgacgcc aaggccttga
     4081 ttcgggcaaa tttcgtctcg aaaccgtcgc tggcacgggc ccacttggcg aggtattcct
     4141 ctgatgcggt cgtgtccggc gcggcaggcg cagaggtttt gggctcttct tcagcctcga
     4201 acatgtcggg acccgcgcca cagacgggac atgcctccgg tggttccggc ccctcgtgga
     4261 cgtacccgca gaccgtgcag acccagcgct ctgttaccgt actcatgcgg cactcctggt
     4321 tgactgagcg aggcgggacg ccgcccgcgg cccgcctctg cgctcaaaac cgctacttgt
     4381 tcccgggtta gtgtatctcg aagaagcgtg aacccggcac gccgcagatc tcgcatttgt
     4441 cgggcacgcc gccgatcacc gtgttgccgc agacggggca cacgtagatc gccgcggcgg
     4501 caaggtcgtt gccggcttca acggccgcca gcgcctcctg gtacagacca tgatgaatct
     4561 cttccacagc catggcattg cggaacgtga tctccgccgg tttgttgccc tcttcctgcg
     4621 ccgctgccac aaattccgga tacatggtct tgaattcatg accttccccg gcaatgccgg
     4681 ccttcaggtt ttcggccgtg gttccaatgc cgcccatgac gcgcagatgg gcgtgcgcat
     4741 gaatcgtctc cgcctcggcc gccgcgcgaa acagtttcgc gacctgcgga aacccatcct
     4801 tctcggcctt ggtggcgaag gcgaggtatt tgcgattggc ctgactctcc ccggcgaaag
     4861 cttcctgaag gttgtccgct gtgctcatga cgtgctccct ttgtgttgcg gctggtgcac
     4921 ccggttcgcg tgcagcgccg catcatgcct gtgccttttg cgctccccgt tgacctctgg
     4981 cttgcgcggg atcccatatg accgcccccc ggcaggctcg agttaagaat tattcttaca
     5041 tgaccccgcc tgcggattca aggccccgtt cttatgccct gtggccactg gcggcgtgtc
     5101 accgtccacc cccggccgct ccgcggccac ccccgctggc gcaggacaca aggcgcggaa
     5161 ccggcagagc tgttggctca cagtctagcc tgcccaatgc atgatgcgtg tgcacgggca
     5221 gacccttgtg cttgcccagt atttgggcca acgcgtgagg atagacggtt gcttaaggct
     5281 tccctggcct attttggttt tcgcagcgcg taggggcaga cccccgtgtc tgcccatctt
     5341 caggctgccg cgtgggttgt ccggatgggc ggccacgggg ggccgcccct acaaccggat
     5401 gcaggcgcgg catggactgc gttgagggac ggcgggcggg ccgttatcat aggttgccgc
     5461 ggcgctgcgc atggcccatc gccgcacagg ccagaggagg cgtaccgcgc atgcacgacc
     5521 tgaaacgtta tggaacctgg gccttgatta ccggagcctc ggcgggcatt gggaaggcct
     5581 tcgcggaggc catcgccgcg caggggttga attgcgtcct ggtggcacga cgcctggacc
     5641 ggctccagga tctcgccgcc gacctgcaat ccaggcacgg ggtggagtgc cggtgtgtga
     5701 atgaggacct ggctcagccc ggctcggcgt atcgcgtggc ggaggccgtc gcagacctcg
     5761 aggtggggct gctgatcaac aatgccggcg tgggttacgc atggccgttt gaagacagcg
     5821 atccggagcg cctggcggac ctgatcaccc tgaactgcgt tacccccgcg ctcctcaccc
     5881 gtttgctggc gccggagatg ataaaacgcc ggcgcggcgg catcatcctg gtggggtcgg
     5941 tgctgggggt tattccatct ccgttccaca cggtatacgg agcgagcaag gcgttcgctg
     6001 ttgcgctcgc ggaaggcctg tacggcgagt atgagcaggc cgggatcgat gtgctcgcgc
     6061 tctgcccctc caccacgcgc acggagttct ttgtggcgga aggcatgtca gaagcgcgcg
     6121 caaaccgtct gatggcgggg gcggaccggc cggaggcgat tgccgaactg gggctgcgct
     6181 acctggggcg caaaccgcgg gtcggcccct gggcgtacac gggcccggcg cttctggcgc
     6241 gtctgctgcc tcgcaaatgg agcatacgcc tcgtccgcaa gttcatgcgg ccgtatctgc
     6301 agaaagacga gaagtccggc tgaaaatcga cagcgcgtca tcacaccgcg tcagcggatt
     6361 tcatgaagcc cctcgtgcat gacgtaatgg cgcttgatgg tgagtttatt catccaggcg
     6421 cggacttcga gggttttcca ggggccggaa tcgggaccgc cgccgttatc gttatcccag
     6481 agccatttcg gcgtaggcca gaggcagaag gacggattta cagcggtgta gaaggcctcg
     6541 gacacgcctc tctggccgtg atgggccatt tgtatgtagt ccgcggggag atgccgggcc
     6601 tggtcgctgt tcaggagctt gtcccctcct tccacgccga gatcgccggt aaacagtacc
     6661 gatttagccg tgtcccagag ccggaacgcg atgcttgaat tgttgatggg attggcggtg
     6721 atctcg
//
LOCUS       DAOPBQ010000151         6692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10169_length_6692_cov_4.025765, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000151 DAOPBQ010000000
VERSION     DAOPBQ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10169_length_6692_cov_4.025765"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="PKW60_06375"
     CDS             <1..155
                     /locus_tag="PKW60_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HPJ99094.1"
                     /translation="GYSTKDIAAQLHLSRKTIETYREKIKEKLNIISGSQLVQRAVQW
                     MLEEGR"
     gene            393..2174
                     /gene="aspS"
                     /locus_tag="PKW60_06380"
     CDS             393..2174
                     /gene="aspS"
                     /locus_tag="PKW60_06380"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPJ99095.1"
                     /translation="MHPFRTHTCAELRKSDVGKEVKLSGWVHRKRDHGGVIFIDLRDH
                     YGLTQVIVNPDAPFFGEAEQVRYESVVTVLGDVVARTPETINANLPTGEIEVVATSFL
                     IESQADPLPFPVNQEVEYPEETRLTYRFLDLRRDRIHKNVLLRSKTAQLVRQHLTERG
                     FIEYHTPILTSSSPEGARDYLVPSRVYPGQFYALPQAPQQFKQLLMASGFDKYFQIAP
                     CFRDEDSRADRSPGEFYQVDIEMSFITQDDLFAELEVLMVRLFRELSSKTITADIFPR
                     IPYRECMESYGTDKPDIRYDLKLRDVSELFAQSEFKVFRSAVDQGGAVKVINAKGAAA
                     QPRKFFDDAEAFAKQEGARGLAWIALRDGEMKGPVAKFLSETEKQALIDKTGAENGDA
                     LFFGAGARAETNALLGKVRIYLANALGLIDTSVVSFCWIVDFPMFEWNEDEKKIDFSH
                     NPFSMPQGGLEALERLDPLDVLAYQYDIVCNGVELSSGAIRNHQPEIMMKAFEIAGYP
                     REVVETKFPALWRAFHYGAPPHGGIAPGFDRIVMLLADEPNIREVIAFPLNQRAQDLM
                     MNAPSEVTQKQLDELHLKIIYPPEEKK"
     gene            2647..4482
                     /locus_tag="PKW60_06385"
     CDS             2647..4482
                     /locus_tag="PKW60_06385"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HPJ99096.1"
                     /translation="MRHRASLVVDMEGMSRPAFHIARELSQNSRSGLTTRFLAKKLDQ
                     PIEEIEYLVDIYDRVFFTDITKVKLVTEAPGAIKRICAGLENHGDVPSLFHGIKSLDS
                     HGFRRLEELLGIERPAGKKSAGEQLLTRCYQHPDSLVEYVATRGFSPVARELFDIIWQ
                     SSDGIMPVAQLRMAHGGSEYDIEQGLWELFRGFALFELFRFDAEGRLLRAVSLLKELR
                     QWRAESERRRTNVGRIKAVRGEIDAVDARGVELSDRLSHLVAAIAAKPARLRGDGDLF
                     REDRRRLLEMVPEDGEPSLNICLWAAQGAGWIARVDNELHAVDVEGLAATERAERHLR
                     LCDTLLTRSSESASRRLIVLLLTDLTVGAWYPVMRFVEYALHRSEESGAPVLRSAGGF
                     WQYLSPGASGSSSRALARSLEETMWWLGLVDRGECEGESVFRLTELGALVLGDGSATL
                     AKALFPSRRSELVVQPNFEIVVDMQAADPLITVPLDQFCERVSTSQVVVYRLTKATFT
                     RAVQQGRNSGAFIEFLLAHNKRAALPRNVLTTLEDWRGGMKRVRLRTIQVLEADDPLV
                     IADLRHRKRVAKYLNPAGSEHMAVLKRTRKSELKKLLEKEGFVVE"
     gene            complement(4591..>6692)
                     /locus_tag="PKW60_06390"
     CDS             complement(4591..>6692)
                     /locus_tag="PKW60_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HPJ99097.1"
                     /translation="YYFAFRLEDKAGNFSPEPSGIGLTSTRADGTPPVITLTGSPSMT
                     IAQNTPFTDPGATATDAVDGNLTPYIVVTGQVDTARPATYTLRYNVSDRAGNAAPEVT
                     RTVTVQPPVAYMLSIPTNLNGPAGATVACPIHISETQGLTSFRITLLFSGSLLQVATV
                     ASGTATSGWGSPVVTAGSGSVSISASGAALSPGSGTVAVLNLAVNQSAAPGQTSTLRF
                     VSASLNGGATPVSIRNGEFTVTPAATYLYGDANGDGRVNHADAVHILKRRTGLIRKPN
                     EPGGEFAEAASDVSGDDPVYAGTVDASLIMRFSEGLISQFPIDTDGNGIGPEATNEKA
                     AVKAAVAQEYGDPAGATRTLNIPGTVTLEPGASYEIPVSVDTGSRTRGYFFELLYDAR
                     ALEYVTIRNGSLTQEWIAPIVNPRAGKIAVANAHVSATSGQGSLAILTFRALPSVTSG
                     ATTHLKFETADLNDGLLYCKECSGAGEPVITALAPQMGAETGGTVVRITGASLRDVSR
                     VLFGNTASPWLRCDAAGNSILAVTPSGGGAVNVTVESPAGTSTLARGFTYFLPQVHLT
                     LAPAGSVVSGRPLEVPVWIVDLSGGQVSTVAFDLCFDPAAFAAKQTDGTFATAEESAL
                     AAGKTVAASLAGPGILRVTVDGAAGKRIGSGLLATCHLLAISAGDDPSSLLYISGTTA
                     ASADAKSLPASASMVND"
BASE COUNT         1299 a         2060 c         2073 g         1260 t
ORIGIN      
        1 agggctacag caccaaagac atcgccgcgc aattgcacct cagcaggaag actatcgaaa
       61 cctaccgaga gaagatcaaa gaaaaactca acattatcag cgggagtcaa ctggtgcagc
      121 gcgcggtcca gtggatgctc gaagaggggc ggtaaaccct tccgctggag cgctgatccg
      181 cccttccaca ggggccggct gtctttcgga cgggcacatc gccattaacc ctggcttttg
      241 tgcgaaggcc ccgcgcagcc cgcgcccgtt cggcggcctt acccgtgcca atttgactta
      301 ggactagcct ctctttacca taccgcaatt catcgcaggc gagttgcgcg agcagtgtac
      361 atggccaaaa accgggcaga tcaggagacg ggatgcaccc attcagaacc catacgtgcg
      421 cggaactccg caaatcggac gtcggcaaag aggtcaagct ttcaggatgg gtccaccgca
      481 agcgcgacca cggcggcgtg atcttcatcg atctgcgcga ccactacggc ctgacccagg
      541 tcatcgtgaa ccccgacgcc ccattcttcg gcgaagccga gcaggtgcgt tatgagagcg
      601 tggtgactgt cctgggcgac gtggtggccc gcacgcccga aaccatcaat gccaaccttc
      661 ccaccggcga gatcgaggtt gttgccacca gtttcttgat tgagtcccag gcggatccgc
      721 tcccattccc ggtaaaccaa gaggtggaat accccgagga aacccgcctg acctaccggt
      781 tcctcgacct ccgccgcgat cgcatccaca agaacgtcct gctccgctca aaaaccgccc
      841 agctggtccg ccagcatctc actgaacgcg gatttattga gtatcacacg ccgatcctga
      901 ccagttcgtc acccgagggc gcgcgcgatt acctcgtccc cagccgcgtg tatccggggc
      961 agttctatgc cttgccccag gccccccaac agttcaagca actgttgatg gcatccggat
     1021 ttgataaata tttccagatc gccccgtgtt tccgggacga ggattcccgt gcggaccgaa
     1081 gccccggcga gttctaccag gtcgacatcg agatgtcgtt catcacccag gacgatctct
     1141 tcgccgagct cgaagtcctc atggtgcgcc ttttccgcga attgtcgagc aaaaccatca
     1201 ctgccgacat cttcccgcgc attccctacc gcgagtgcat ggaatcgtac ggcaccgaca
     1261 agcccgacat ccggtacgac ctcaagctgc gcgacgtctc cgagcttttt gcccagagcg
     1321 aattcaaagt gttccggagc gcggtggacc agggcggcgc cgtcaaggtc atcaacgcca
     1381 aaggcgccgc cgctcagccc cgcaagtttt tcgacgatgc cgaagccttc gccaaacagg
     1441 agggcgcccg cggcctggcc tggatcgcgc tgcgcgatgg cgaaatgaaa ggccccgtgg
     1501 ccaaatttct cagcgagacc gagaagcagg cgctcatcga caaaaccggc gcggaaaacg
     1561 gcgacgccct ctttttcggg gcgggagcgc gcgcggaaac caacgcgctc ctgggtaagg
     1621 tgcgcatcta cctggccaac gccctcggcc tcatcgatac cagcgtcgtt tcattctgct
     1681 ggatcgttga ttttccgatg ttcgaatgga atgaagacga aaagaaaatc gacttctcgc
     1741 acaatccatt ctcgatgccc cagggcggat tggaggcgct ggaaaggctc gacccgctcg
     1801 acgttctcgc ctaccagtac gacatcgtct gcaacggcgt cgaactttcc tcgggcgcca
     1861 ttcgaaacca ccagcccgag atcatgatga aagccttcga aatcgccggc tacccccgcg
     1921 aagtggtcga aaccaaattc cctgcgctgt ggcgcgcttt ccattacggc gccccgccac
     1981 acggggggat cgcgccggga tttgaccgca tcgtcatgct tctggccgac gagcccaaca
     2041 ttcgcgaggt catcgcgttc ccgctgaacc agcgcgccca ggacctcatg atgaacgccc
     2101 ccagcgaagt cacccagaaa cagctggacg agctccatct caagatcatc tatccgcccg
     2161 aagagaaaaa atagcccgcg accggctgcc tgcccgcatt ccgcctgctg gatcctgagc
     2221 ggctgtgcct gtcgcgccat gtactctcta accaggcatg gaaggaagcg caggggcttg
     2281 cccctacaca tgcggcacgc gtggccgggg gtggactttc atctttcatt tccgatacgt
     2341 gcctgaacag cttctatggc ggccgtagct ggccagagcc caaaacgcgc ccaacagcac
     2401 cgagcggcac cctcagcctt tcctggcata acgcctccaa tgcatgtctg aatcgcccaa
     2461 aaatcggatt cccggcacta tccagcctcc gcggttgcgg cacgatcgaa aatgccgcaa
     2521 caaagccatc atcggccaga ccctggccag atgcgcttca ttcgttctct tgtcaacccg
     2581 gccgttgtgt actatactga ttcttcgtat tctttttttg aaaaataccc ataaacaggg
     2641 ggttttatga ggcaccgggc cagtctcgta gtcgatatgg aagggatgag ccgtcccgcg
     2701 tttcacatcg ctcgtgaact gtctcaaaac agccgttccg gtctgacaac ccggtttctg
     2761 gccaaaaaac tcgaccagcc gatcgaggag atcgaatacc tggtcgacat ctacgatcgg
     2821 gtgtttttta ccgacattac caaggtgaag ctcgtgacgg aggcgcccgg cgccatcaag
     2881 cggatctgcg ctggactcga aaaccacggc gacgtgccgt cgctgtttca cggcatcaaa
     2941 tcgctggatt cgcacggatt tcggcggctg gaagagctcc tgggcattga gcggcctgcc
     3001 ggcaagaaga gtgccgggga acagctgctc acccggtgct accagcatcc cgactcgctc
     3061 gtcgaatatg tggcgacccg cggattttcc cctgtcgcgc gcgaactgtt cgatatcatt
     3121 tggcaaagca gcgacggcat catgccggtg gcgcagctgc gcatggcgca tggcggttcg
     3181 gagtatgaca tcgagcaggg gctctgggag cttttccgcg gtttcgccct gtttgaattg
     3241 tttcgtttcg atgccgaagg ccgcctgctg cgtgccgtga gcctgcttaa ggaattgcgc
     3301 cagtggcggg ccgaatctga gaggcgccgc accaatgtcg ggcgaatcaa agcggttcgc
     3361 ggcgagatcg acgccgtgga tgcgcgtggc gtggagttga gcgatcgtct gtcacacctg
     3421 gtggcggcca ttgcagcgaa accggcccgg ttgcgcggag acggcgacct cttccgcgaa
     3481 gaccgccgcc ggctattgga aatggtccct gaagacggcg aaccttccct caacatctgc
     3541 ctctgggcgg ctcagggcgc ggggtggatt gcgcgtgtag acaacgaatt gcacgcggtc
     3601 gacgtggaag ggcttgcggc cactgaacgg gctgaacggc acctgcgcct gtgcgacacg
     3661 ctcctgactc gcagcagcga atcggcgtcc cggcgcctca tcgtgctgct gctgaccgac
     3721 ctgacggtgg gcgcctggta tccggtcatg cgctttgtgg agtatgccct tcaccggagc
     3781 gaggaaagcg gcgcgccggt gcttcgttct gccggaggtt tctggcagta cctgtcgccc
     3841 ggcgcttccg gctcatcgag ccgcgcgctg gcgcgttcgc tggaagagac gatgtggtgg
     3901 ctcggcctgg tggatcgggg ggaatgcgag ggcgagagcg tgttccgcct cacggaactg
     3961 ggcgctctgg tcctgggcga tgggagcgcc accctggcga aggcgctctt tccttcccgg
     4021 cgctccgaac tggtggttca gcccaatttt gagatcgtcg tggatatgca ggcggcggat
     4081 cccctcatca cggtgccgct cgaccagttc tgcgaacggg tgagcaccag ccaggtggtt
     4141 gtgtaccgtc tgaccaaagc gaccttcacc cgggctgtgc agcagggccg aaacagcggc
     4201 gcgttcatcg agtttctcct ggcccacaac aagcgcgccg ctctgccgcg caacgttctg
     4261 accacgctgg aagactggcg cgggggaatg aaacgcgtcc ggttgcgcac cattcaagtg
     4321 ctcgaggccg acgatccgct cgtgattgcc gatttgagac accggaaacg ggtggccaag
     4381 tatctgaatc ccgccggatc tgaacatatg gccgtgctca aaagaacccg gaagtctgaa
     4441 ttgaaaaaat tgctggaaaa agaagggttt gtcgtcgagt gatggcgccc gcaacctctc
     4501 attcggcctg gctgagataa ggcgacggcg ggcgcgccct cccgggagga acacgcccac
     4561 cgtcttctcc ctgcggtggg tcgcaagagc tcagtcgttt accatgctcg cagacgcggg
     4621 aagcgatttg gcgtctgcgc tggcagcggt ggttcccgag atgtaaagca ggctggacgg
     4681 atcatcgccg gcggagatcg ccaacaaatg gcaggtcgcg agaagaccgc tgccgatgcg
     4741 ttttccggct gcgccgtcta cggtcacgcg caggatgccg gggcctgcca ggctcgcggc
     4801 cacggtttta ccggccgcaa gcgcggattc ctcggctgtc gcaaaagtcc catccgtctg
     4861 ctttgcagca aaggccgccg gatcaaagca cagatcgaag gcgacggtgc ttacctggcc
     4921 gccactgaga tccactatcc acaccggcac ctcgagaggc cgtccggaga ccacggagcc
     4981 tgcaggagcc agggtcaagt gaacctgcgg gagaaaatag gtgaagccgc gcgccagcgt
     5041 ggatgtgccc gccgggcttt ccaccgtgac attcactgcg ccgccgccgg acggcgtcac
     5101 agccagaatc gaattgcccg ccgcatcgca gcggagccag ggagaggccg tgttcccgaa
     5161 caaaacgcgc gagacgtctc tgaggctggc gccggtaatg cgcacgacgg tcccgcccgt
     5221 ctctgcgccc atctgagggg ccagagcggt gatgaccggt tccccggccc ctgaacactc
     5281 tttgcagtag agcagcccgt cgttcaaatc agcggtttca aatttgaggt gggtcgtcgc
     5341 acctgaggtc acggatggca gggctcgaaa cgtgaggatg gccaggctgc cttgtccgga
     5401 ggtcgcgctg acatgggcat ttgcaacggc aattttgcct gcgcgcggat tcacgatggg
     5461 cgcgatccat tcctgggtga gggaaccgtt gcggatggtc acgtactcca gggcgcgcgc
     5521 gtcgtaaagc aattcgaaga agtagccgcg ggtacgcgag ccggtgtcaa cgctgacggg
     5581 gatttcgtag cttgcacccg gttccagcgt cactgtgccg gggatgttga gagtccgcgt
     5641 ggcccccgcc ggatcgccat attcctgggc gaccgcggct ttgaccgcag ccttttcgtt
     5701 ggtggcttca ggtccgatgc cattcccatc ggtatcgatg ggaaattggc tgatcagtcc
     5761 ctcgctgaac cgcatgataa gggacgcgtc aaccgtcccg gcgtagaccg ggtcgtcccc
     5821 gctgacatcg ctcgcggcct cggcaaactc tcccccgggt tcattgggtt ttctgatcaa
     5881 cccggtgcgg cgcttcagga tgtgcacagc gtcggcatgg ttgacgcggc cgtcgccgtt
     5941 tgcgtcgcca taaaggtagg tggcggctgg agtgaccgtg aactcgccgt tccggataga
     6001 tacgggggtg gcgccgccat tcagcgacgc ggagacaaag cgcagcgtgg aggtctgacc
     6061 gggggccgcg gattgattga cggccaggtt caatacggcc acggtcccgg aaccggggct
     6121 cagggcggca ccagacgctg agatggacac cgagcccgaa ccggccgtaa cgaccgggct
     6181 gccccagccg ctggtggcgg tccccgatgc cactgtggcg acctgaagca gactgccgct
     6241 aaatagcagg gtgatgcgga acgatgtaag gccctgcgtc tcgctgatat gtatcgggca
     6301 cgccacggtg gcgccggcgg ggccgttgag gttggtgggt atggagagca tgtaggcgac
     6361 tgggggctgg accgtcaccg tgcgggtaac ttccggggcg gcgttgccgg cgcggtcgga
     6421 cacgttgtat cgcagggtgt atgtggccgg cctggcggta tcgacctggc ctgtgaccac
     6481 gatgtagggg gtcaggttac cgtccacggc gtcggtggct gttgcgcccg gatcggtgaa
     6541 gggggtgttt tgtgcgatcg tcatactcgg cgagccggtc agagtaatca ccggaggagt
     6601 gccgtcagcg cgcgtgctgg tcaaaccgat tcctgaaggt tcgggactga aattgcctgc
     6661 cttgtcttcg aggcggaacg caaaatagta ca
//
LOCUS       DAOPBQ010000152         6682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10187_length_6682_cov_4.904783, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000152 DAOPBQ010000000
VERSION     DAOPBQ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10187_length_6682_cov_4.904783"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PKW60_06395"
     CDS             complement(<1..943)
                     /locus_tag="PKW60_06395"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99098.1"
                     /translation="MAGISGRHDFGDELVRQEVLTREELDAIMEQEAVSGVPWYRQLL
                     QKEIISIGALEDLFKFEYHSRAKRKLDESIGATLVKMEEISKDDLKAALAEQKRTGRL
                     LGKILLDSGVVKPEALAKALAKQHKLEYAVLSKTASTAEALDAIPESLARKYRFIPVR
                     IDGDRLSVLINDPRVRDSLESAEVFLGKRIHPILTHVDDIEEESKKRYRSRKTAPLKE
                     PQTEPPKPAKDAAPKPAPKKETPSKTATQGTEQKDDVVMAEEIAVERSTMDFAEIARQ
                     ASGASVMQLVTKMIEGAVNSGATDIHLDPQEPEMRVRY"
     gene            complement(1060..1914)
                     /locus_tag="PKW60_06400"
     CDS             complement(1060..1914)
                     /locus_tag="PKW60_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ99099.1"
                     /translation="MRSHKAIWTITAAIVALAVFVAVMRKPAVPHSAPYLVDLSPAQS
                     RLLIDTMLVCLDEDPQARRIRTRYLVEAVAALAEEGGVQTAETCFAQGVLRYGQRWKN
                     APLAKSETAFRQAIELRPDWALPYNGLGLTLMHLERYEEAEAAYRHAIQLDPKWSRPH
                     NDLAVLLRHTKRLDEAEAEALIALSLDPDSVANNNNYGNVLLKRGRVEEAEAQYRKAI
                     TLDPQEPAPYYNLACTAARRGDLDGMLAYLNVAIVLGEVFREEASEDEEFAGYRNHPD
                     FRKLVYRK"
     gene            complement(2078..2935)
                     /locus_tag="PKW60_06405"
     CDS             complement(2078..2935)
                     /locus_tag="PKW60_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPJ99100.1"
                     /translation="MIRVGPAGWSYPDWKGIVYPPEMPRQLHPLEYLSRYFDTIEVNA
                     SFYRPFPPGNAERWVNLLRGNPGFKFTAKLYRAFTHERESWPQQPDIDAVRAGFDVLM
                     GAQRLGAVLVQFPWSFKRTVENRQWLARVLGTFSEYPLALEVRHASWNRPEVFDELAR
                     REIAFCNVDQPLFSGSLGPTARVTARVGYVRLHGRNAGDWFREDAGRNERYDYLYSLE
                     ELNPWLQKIERLRERAAELYVITNNHYRGQAVVNAFELQTALGIQKGARLPESLRDAY
                     PRLSGASSG"
     gene            3330..3938
                     /gene="rpsD"
                     /locus_tag="PKW60_06410"
     CDS             3330..3938
                     /gene="rpsD"
                     /locus_tag="PKW60_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPJ99101.1"
                     /translation="MGRYTGPKHKLCRRLGSCVWGDPKCPAIKRPYAPGQHGPTLRRK
                     ATVYGQQLLDKQRIQTHYGLLEKQMRRTFARAQRMGGVTGSNLLLILESRLDAVVYRL
                     GFARTMQQSRQIVTHGHILVDGQKCNMPGFQVKPGMTVSIKEKSRKVPVLAEGAANPP
                     AVIPEYLDRATDSFEGKMIAAPNIETIPFKPDTAGIIGFYSR"
     gene            4039..>6682
                     /locus_tag="PKW60_06415"
     CDS             4039..>6682
                     /locus_tag="PKW60_06415"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPJ99102.1"
                     /translation="MALVFPTLQAVGEAARVLGGQPGTHVEIVGPWGSGKSLLAVQLA
                     QARGASLLIAASSRADADAIYEDLCSFLPGEQCLQFPAWEVLPTDSMEPADDIVAERM
                     NALMRLSEALERSTPVACVAPVQSLVQRVAPHSRIQEQSLTLRVGDEQEIDALLLKLT
                     DMGYERELMVERRGQMSLRGGIFDIFPIASELPLRIEFFGDEIESMRRFEPETQRSVG
                     RAGQVRILPRSEKQLLQEGAGGPGLGTLLEYLPDSALLVLDEPARLAEEAEALSRLHA
                     DSRYFIPWSELQQAFEAFSCVSLAQVAPGGPGQLPRIAAPMRSITGWERDRQAFWDQL
                     TRWDADGYRVRIFCANAGERRRLHELLEEQGHRLGQDAFDLRLELGRLRGGFASPADK
                     LTVLSEREMFGRHYVRRQRRRFEAGAAITSFDDFRAGDYIVHARHGIGRYLGLRRFEG
                     KAGDFLALQYLGGDRLYLPVTHIDEVQKYQASEGAAPRIDKLGGSTWARKKARVKKAV
                     RDMTEELLKLYAAREARDGHAFSKDTHWQGEFEDAFEYEETPDQARAILEVKRDMEFP
                     RPMERLLCGDVGFGKTEVAIRAAFKAVMDGKQVAVLAPTTVLAEQHYQTFLQRMADYP
                     VTVEMLSRFRSPGQQKITIERLQSGEVDIVIGTHRLTSKDIKFKDLGLVVIDEEQRFG
                     VAQKEKLKHLRNTVDVLTMSATPIPRTLNLALLGARDMSVINTAPNDRLPVHTCIEQF
                     DEQLIREAIERELAREGQVFFLHNRVQTIMSYATLVRKLVPLARVAVAHGQMHENELE
                     RIMSAFVRREIDVLVCTTIIGSGIDIPNANTIIITNATNFGLAELYQLRGRVGRYKHR
                     AFAYLLVPGDRALSEVAQQRLKALE"
BASE COUNT         1231 a         2024 c         2085 g         1342 t
ORIGIN      
        1 ggtaccgcac gcgcatctcc ggctcctgcg gatcaaggtg gatgtcggtt gcgcccgaat
       61 tgaccgcgcc ttcgatcatc ttggtcacca gctgcataac ggacgcgcct gatgcctggc
      121 gcgcgatctc cgcgaagtcc atcgttgagc gttccactgc gatttcttca gccatcacga
      181 cgtcatcctt ctgttccgtg ccttgggttg ctgttttgga aggggtctct tttttcgggg
      241 cgggtttcgg ggcggcgtcc tttgccggtt tgggaggttc ggtttgtggt tccttcaacg
      301 gtgcggtctt gcggcttcga tagcgtttct tgctttcttc ctcaatgtcg tcaacatggg
      361 tgaggatggg gtgaatgcgt ttgcccagga aaacttcagc cgattccagc gaatcccgga
      421 cccgcgggtc attgatcagc acggacaggc gatcgccgtc gattcggacc ggaataaacc
      481 ggtattttct ggccaggctt tcggggatgg catccagggc ttcggccgtg ctcgcggttt
      541 tgctcagcac ggcatattcc agtttatgtt gtttggccag agctttcgcc agcgcctcgg
      601 gtttcacgac gccgctgtcg agcaggattt ttcccaggag ccggcccgtt cgtttctgct
      661 ccgccagggc tgctttcagg tcgtcctttg agatttcctc catcttcacc agcgtcgcgc
      721 cgatcgattc gtcgagcttt cgttttgccc gggagtgata ttcaaacttg aacaagtcct
      781 ccagtgcgcc gatgctgatg atttcctttt gaaggagctg ccggtaccag gggacgccac
      841 tgaccgcttc ctgctccata atggcgtcga gttcttcgcg agtcagtact tcctggcgta
      901 cgagttcgtc gccaaaatca tgccgtccag atatacctgc catgattacc cccttctaca
      961 agcttgggcg ccggtaaaat tgcgatacgc cattatattc aggcaacgcg ggaacgtaaa
     1021 gggatatcgc tgtgttcaca agcgcgccgg tggcgtttcc tacttgcgat aaacgagctt
     1081 acggaagtcc ggatggttgc ggtagccggc aaattcctcg tcttcgctgg cttcttcgcg
     1141 aaacacctcc ccgagcacga ttgccacgtt gagatacgcc agcatgccgt ccagatctcc
     1201 gcgccgcgcg gcggtgcacg ccagattgta gtagggggca ggttcctgcg gatccagcgt
     1261 gatggctttg cggtattgcg cctcggcctc ctcgacgcgc ccgcgtttca acaggacgtt
     1321 cccgtagttg ttgttattgg ctacgctgtc ggggtcgagg gagagcgcga tcagcgcttc
     1381 ggcttccgct tcatccagcc gtttcgtgtg gcggagcagc accgccagat cgttgtgcgg
     1441 gcggctccat ttggggtcga gctgaattgc atggcggtat gcggcctcgg cttcctcgta
     1501 ccgttccaga tgcatcagtg tgaggccgag cccgttgtac gggagcgccc agtccgggcg
     1561 cagttcgatg gcctgtcgaa aggccgtttc cgatttggcg agcggggcgt ttttccagcg
     1621 ctgaccgtag cggagcacgc cctgggcaaa gcaggtttcc gccgtttgca cgcccccttc
     1681 ttctgccagt gcggcaactg cttccacaag ataccgcgtc cgtatccgcc gcgcctgggg
     1741 atcttcgtcc aggcacacca gcatcgtgtc gatcaggagg cgggattgcg ccggcgacag
     1801 gtctaccagg tacggcgcgc tatgaggcac agccggttta cgcatgacgg ccacaaacac
     1861 tgccagagcg acaatggccg cggtaatggt ccaaatagcc ttatgtgacc gcatgaattc
     1921 ttgccagacc ccatttaccc cgggtctacc gtacatcgaa tttgaaaaaa tccttcggaa
     1981 accgcccttt caactcggcc gtgcttcctg ttcggaccga agcataatat catgctgctc
     2041 aacccgttgc gagcttaatt ccgcagaaaa acgcagtcta tcctgatgag gcgccgctca
     2101 gacgcggata ggcgtcacgc agcgactcag gcagacgggc gcccttctga atgcccagcg
     2161 cggtttgaag ctcgaacgcg ttgaccaccg cctggccgcg atagtggttg tttgtgatga
     2221 cgtacagctc agcagccctt tcccggagac gttcgatttt ctgcaaccac gggttcagtt
     2281 cttccagact atacaagtag tcgtagcgtt cattgcgccc cgcatcctcg cggaaccagt
     2341 cgccggcgtt ccggccgtga aggcgaacat aacccacccg ggcagtcacc cgtgcagtcg
     2401 ggcccaggga accggaaaat agcggctgat cgacattgca gaacgctatt tcgcgccggg
     2461 cgagttcgtc aaagacctca gggcggttcc aggacgcgtg gcgcacttcc agtgccaggg
     2521 gatactctga aaatgtcccc aaaacccgag ccagccactg gcggttctcg accgtccgct
     2581 tgaaactcca gggaaactgc accagcaccg cacccagacg ttgtgcaccc atcaacacgt
     2641 cgaatccggc gcgcacggcg tcgatgtccg gctgctgcgg ccaggattcc cgttcgtggg
     2701 tgaaggctct gtataatttc gccgtgaatt tgaagccagg gtttccgcgc agaagattga
     2761 cccagcgttc cgcgttgccg ggtgggaacg ggcggtaaaa cgacgcattc acctcgatcg
     2821 tgtcgaaata gcggctcagg tactcgagag ggtgcagctg gcgcggcatc tccggcgggt
     2881 agacgattcc cttccagtcc gggtaggacc atcctgccgg gccgactcgt atcactgcgc
     2941 tgcctgctgt catcgcgtgc tgctccgggc gccagagtac cacgccccgg aaagccggcg
     3001 ttaacgcggt tctgcggacc ggtgtgccgg gtttgcccct ggcaaaagca gttggcaagt
     3061 tttccagggt gcatggccgc gtcatttgcc aaatcttgcc ggggtgcgcg actcgtacca
     3121 tccccggtaa gatcttagat ggcgcgattc gtgcgcttga cttgggtcgc gaaaatacgc
     3181 taagctactg tgcttgccgt gtgatagcgt gaaaaagagg gtcacattca acggcggaat
     3241 atcgcttgtt ccatgcgtat acccggcacg gaagcaggtg tgtttttcgg aaaagcgggt
     3301 aatccaggga aaaactgaaa aggaccatca tgggcaggta cacgggacca aaacacaagc
     3361 tgtgtcgtcg actgggctct tgcgtgtggg gcgatccgaa gtgccccgcg atcaaacgcc
     3421 cgtatgcgcc gggccaacac ggcccgacct tgcggcgcaa ggctacggta tacggccagc
     3481 aattgttgga caaacagcgg atccagacgc actacgggct gcttgaaaag cagatgcggc
     3541 gcacgttcgc ccgggcgcaa cggatgggcg gcgtgacggg cagcaacctg ctgttgattc
     3601 ttgaatcgcg tttggacgcg gtggtgtacc gccttgggtt cgcaagaacc atgcagcagt
     3661 cgcgccagat tgtcacccac ggccatattc tcgtggacgg ccagaagtgc aatatgcccg
     3721 ggtttcaggt gaagcccggc atgaccgtca gcatcaagga aaagagccgc aaggttcccg
     3781 tgctcgcaga aggggcagca aatccgcccg cggtcattcc agagtacctc gaccgggcca
     3841 ccgattcgtt tgagggcaag atgatcgctg cgccgaacat tgaaaccatc ccgttcaagc
     3901 cggacaccgc cgggatcatt ggtttctact cgcgctgagc cgcagtggcg tgttttccgg
     3961 gcgggccgtg ccagcggccc gcccgtcgtg tctatctcac ccgcgtaccg cccggctggt
     4021 cgcggcatcg cgtgaaccat ggcgctcgta ttccctacat tacaggcggt cggagaggcg
     4081 gcgcgcgtgt tgggcggcca gcccggcacg cacgtcgaga tcgtggggcc ctggggttcg
     4141 ggtaaatctc tccttgccgt gcaattggcc caggcacgcg gggcgtcgct cctgatcgca
     4201 gcgtcgagcc gcgccgacgc cgacgccatc tacgaagatc tgtgcagttt cctgcccggg
     4261 gagcagtgtc tccagttccc ggcctgggaa gtgttgccga cggactccat ggagcccgcc
     4321 gacgacattg tggccgagcg catgaatgca ctgatgcgcc tgtcagaggc gctggagcgc
     4381 agcactccgg tggcctgcgt ggcgccggta cagtcactgg tgcagcgggt agcgccgcac
     4441 agccgcatcc aggagcagag tctgaccctg cgcgtgggcg acgagcagga gatcgacgcc
     4501 ctgctcctga agctcacgga catgggctat gaacgggagc tgatggtcga gcgccggggt
     4561 cagatgagtc tccggggcgg cattttcgat atctttccca ttgcgagcga gcttccgctg
     4621 cgtattgaat ttttcggcga tgaaatcgag tcaatgcgcc gttttgagcc cgagacgcag
     4681 cgcagtgtcg gtcgcgccgg gcaggtccgg atcctgcccc gctccgaaaa gcagcttctc
     4741 caggaaggcg ctggaggccc ggggctgggc acgcttttgg aatacctgcc ggattcggcc
     4801 ctgctcgtcc tcgatgagcc ggccaggctc gccgaggagg ccgaggccct gtcgcggctg
     4861 catgcggaca gccgctattt catcccgtgg agtgagctcc agcaggcgtt cgaggcgttc
     4921 tcgtgcgtga gtctggccca ggtagccccg ggcggcccgg ggcagctgcc gcgcatcgcc
     4981 gcgccgatgc gctcgatcac cggctgggag cgggaccggc aggcgttctg ggaccagctc
     5041 acacgatggg atgctgacgg ctaccgggtc cgcatcttct gcgcgaatgc gggcgagcgg
     5101 cggcggctcc acgaactcct cgaagaacag ggccaccggc tcgggcagga tgcgttcgat
     5161 ctgagactcg agctcgggcg tctgcgcggc ggatttgcgt ctcccgccga caagctgacg
     5221 gtgctgagcg agcgggagat gttcgggcgg cattacgtcc gccggcagcg gcgccgcttc
     5281 gaggcgggcg ccgcgatcac ctcgttcgac gatttcaggg ccggggacta tatcgtgcat
     5341 gcgcgccacg gcattgggcg ctatctgggg ctgcgccgtt ttgaaggcaa ggccggcgat
     5401 ttccttgcgc tgcagtattt gggcggcgac cggctttacc tgcccgtcac acacatcgac
     5461 gaagtgcaga aataccaggc gagcgaaggc gcggccccgc gcattgacaa gctgggcggc
     5521 tcgacctggg cgcgcaaaaa ggcccgggta aaaaaagcgg tgcgcgacat gaccgaggaa
     5581 ctcctcaagc tctatgccgc gcgcgaggcc cgcgacgggc acgccttctc gaaagacacc
     5641 cactggcagg gggaatttga agatgcgttc gaatacgagg aaacccctga ccaggcgcgc
     5701 gccattctcg aagtaaaacg cgatatggag tttcccaggc ccatggagcg gcttctctgc
     5761 ggcgatgtgg ggttcggcaa aaccgaggtg gcgatccgcg cggcattcaa agcggtaatg
     5821 gacggtaaac aggtggccgt gctcgcaccg acgacggttc tcgcggaaca gcactaccag
     5881 acgtttttgc aacgcatggc cgactatccc gtcacggtgg agatgctgag ccggttccgt
     5941 tcgccggggc agcagaaaat caccatcgag cgcctgcaat cgggcgaagt cgacatcgtg
     6001 atcggcacgc accgcctgac atccaaagac atcaagttca aggatctcgg gctggtggtg
     6061 atcgacgagg agcagcgctt cggcgtcgct cagaaagaga agcttaaaca cctgcggaac
     6121 acggtcgacg tgctgaccat gagcgcgacg cccattcccc ggacactcaa tcttgccctg
     6181 ctcggcgcac gcgacatgag tgtcatcaac accgcgccca acgatcgcct gccggtgcac
     6241 acctgcatcg agcaattcga cgaacagctt atccgcgagg ccatcgaacg cgaactggct
     6301 cgcgaagggc aggtgttttt cctccacaac cgcgtccaga ccattatgtc gtatgcgacg
     6361 ctggtgcgga agctcgtccc gcttgcccgg gtggcggttg cccacggcca gatgcacgaa
     6421 aacgagctgg aacgcatcat gtccgccttt gtccggcgcg agatcgatgt gctggtctgt
     6481 acgaccatca tcgggtcagg catcgatatt cccaatgcca acacgatcat catcaccaac
     6541 gccacgaatt tcggcctggc tgagctgtat cagctgcgcg gccgcgtggg ccgttacaag
     6601 caccgggcct tcgcgtacct cctcgtgccc ggcgaccgcg ccctgagcga ggtggctcag
     6661 cagcgtctca aggcgctgga gg
//
LOCUS       DAOPBQ010000153         6678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10190_length_6678_cov_3.632644, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000153 DAOPBQ010000000
VERSION     DAOPBQ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10190_length_6678_cov_3.632644"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKW60_06420"
     CDS             <1..368
                     /locus_tag="PKW60_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HPJ99103.1"
                     /translation="TLDFSFSGLKTAVLYRLREGGVSLEDIAASFQAAAVDTLLLKTR
                     QAVEQTGAQRLVLAGGVAANRLLRTRLAGELGIPVTMPPFDLCVDNGAMIAAAGFSRY
                     QQGFLGDTRTRPHPGLRLA"
     gene            465..1532
                     /locus_tag="PKW60_06425"
     CDS             465..1532
                     /locus_tag="PKW60_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPJ99104.1"
                     /translation="MNLHDLMREAADSNASDIHLKTNNPPLLRIDGDLIRLEAEALDA
                     DDIMTMLNEIVLPHEVEKFKKVKELDTSYMLENIARFRVNACFDDGNPRLVLRTIPLD
                     IKEIDELGLPHVLHRICQLNNGLVLFTGVTGSGKSTSLAAMVDEINRTRPVHTITIED
                     PMEFLHYDKIGIVTQREVGQDTLSFANALRGALRQDPDVILIGEMRDAETVRTAISSA
                     ETGHLVFSTLHTVNAVETLNRIMDFFEPHQQLQIRKQLASVLRAVCSQRLLPSIEGHG
                     RCVAAEILVGNRTVREYIEQGKSFNDLTKLIEDGFEQYGMQTFDQALFVLWKEKRIDE
                     QTALDNSTSPRDLKLRMEGFR"
     gene            1779..2642
                     /locus_tag="PKW60_06430"
     CDS             1779..2642
                     /locus_tag="PKW60_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99105.1"
                     /translation="MLLLVLSATGGNLLLNPGFESVRDNAPLRWDLYVMPRAGCQGEL
                     DSSIAHEGQYSVKLHNETPYGKEPANNWSQSITTNLRNRELRVSGWLKTQGATEAALW
                     LQCFQRNPLRILALESTTHAQLITGDTDWTEVEMRVTVPNETDFVMFRCVLLGEGTAW
                     FDDVSVEDAPEHISTPPEAAVAEAVRKEAPPQAPPASKDSAQLEELRISRQALADANR
                     TLRELNTSLLTQITELQVEIEQLRQEIQDLRLQEEAERTEAPPTPSVPQPEMPVPPLV
                     PHVPPIEKEQP"
     gene            2639..2788
                     /locus_tag="PKW60_06435"
     CDS             2639..2788
                     /locus_tag="PKW60_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99106.1"
                     /translation="MNLVLVISILAGLSAAGLVWFGWSLLAAIAPRSSPVSSNPGGDE
                     PGDDE"
     gene            2785..2997
                     /locus_tag="PKW60_06440"
     CDS             2785..2997
                     /locus_tag="PKW60_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99107.1"
                     /translation="MRDSAEGERKPPAKRNRPFLGVLFTCCHVYTRIYLNPAGTAFTG
                     FCPKCGGKLTVHARKGGSKSRFWTAG"
     gene            complement(3062..3943)
                     /locus_tag="PKW60_06445"
     CDS             complement(3062..3943)
                     /locus_tag="PKW60_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPJ99108.1"
                     /translation="MALLMESKTFYASHSEHSEPGEYAELLQRLPDEVGALCEVIQGL
                     LIHVFHAAKYGVEIAPERMQEMHLYGMEEMLERLFELDPAPLTVARAPERRLVATCRD
                     YSLFLCAILRAHGRAARLRTGFESYFHRKYNRFGDHTLCEYWDESRERWICADAQLDA
                     VQRQAFKIDFDALDLPAEEYAVSGRVWLECRNGARDPRKCGMLHRWGLDYVRGNVAKD
                     IMTLNKVELFPWDGSVLGDKPRGELTEADLALLDELAGLTWPEVNSQAVRAHYAQNKD
                     LHGINMLLKLRGLDPAV"
     gene            4065..5828
                     /locus_tag="PKW60_06450"
     CDS             4065..5828
                     /locus_tag="PKW60_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99109.1"
                     /translation="MLFAVNLLCLAAAQLNLLTDPSFEEALTHWEHGALHARMDSLQV
                     DGRSVARITVPGDAPVGWPSIAQRIPVTPGDLLEGAAGVLAQGIQDGAGAYLAIEFHD
                     AAGERLSYAQSRGLLCEPEWQTLTVRAVAPGGATAARLCLILNGRGEALFDDARLTPL
                     GNLTPEPLEGGVQITVTDEVVCPSLIGFGAEDDGWFYAPINLERGITADDIALREKRI
                     EWLDPDWIRMFFWYRDWNPSGDWTSFTFDSPNMQSHYRTLELYQRIGAAVNVTGVEWQ
                     LNDPFGHPTEMSHAIGTLMEHLIKTRGFTCIRQWTLTNEPNSHFIQLGYPFERYVELH
                     RLVKAEFARRGLDVQIVGSDDTAGFEWFQACVNNDAYFDAADLFASHRYFPYADRVLA
                     PFFFEDRQAALAKRSPAKPFVVAEFGFQDHRAGTLANPLMEDYSYALWTYAFIVDGLN
                     RGVAGFSIWCMHEVYYPGNGFMNYGLWNYRDRGWGIRPVYHALGCFTRMTHAGDAVRK
                     CVSSHPAYVSAAVAGTRVFWVNQADNEVSVACSAPLTGPATAYTRDSVATDDSPGIDI
                     TVEGNRFVAPPQSFGYLCLED"
     gene            complement(5972..6196)
                     /locus_tag="PKW60_06455"
                     /pseudo
     CDS             complement(5972..6196)
                     /locus_tag="PKW60_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153206.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
     gene            6608..>6678
                     /locus_tag="PKW60_06460"
     CDS             6608..>6678
                     /locus_tag="PKW60_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPJ99110.1"
                     /translation="MRILVTGGAGFIGSHLCEALVSQG"
BASE COUNT         1297 a         2184 c         1916 g         1281 t
ORIGIN      
        1 acactctcga tttcagcttc agcggcctga aaaccgcggt gctgtatcgc ttgcgcgaag
       61 gcggcgtctc ccttgaggac attgccgcca gctttcaggc agccgccgta gacaccctgc
      121 ttctcaaaac ccgccaggcc gtggaacaga ccggcgccca gcgcctggtc ctcgcgggcg
      181 gggtcgcggc caacagactc ctgcgcaccc ggctcgccgg agagctgggc attccggtga
      241 ccatgccgcc gtttgatctc tgcgtcgaca atggcgcaat gattgccgcc gcgggatttt
      301 cgcgctacca gcagggcttt ctgggggata cacgcacccg gccccacccc ggattgcggc
      361 tggcataagg cacattctcc caaaaacatg gtatgcttta ggcggctcac cagggtttgg
      421 gggtgttccc ggaaaacgcg tttccatgtg aggagtgctg aagcatgaat ctgcacgatc
      481 taatgcgcga ggccgccgac tccaatgcct ccgacatcca tctcaagacc aacaacccgc
      541 ccctgctccg catcgacggt gacttgatcc gcctggaagc cgaggcgctg gacgccgacg
      601 atatcatgac catgctgaat gaaatcgtgc tgccgcacga agtcgagaaa ttcaagaaag
      661 tcaaagaact cgacacgtcc tacatgctgg agaacatcgc ccggtttcgc gtgaatgcct
      721 gtttcgacga tggcaacccc cggcttgtcc tccggaccat tccgctcgac atcaaggaga
      781 tcgatgagct ggggctgcca catgtgctgc accggatctg ccagttgaac aacggcctgg
      841 tgttgttcac cggggtcacc ggcagtggaa aatccacctc cctcgccgcg atggtggatg
      901 aaatcaaccg gacccggccc gtgcatacca tcaccatcga ggacccgatg gagtttttgc
      961 actacgacaa gatcggcatc gtgacccagc gcgaggtggg gcaggacacc ctgtcgtttg
     1021 ccaacgcgtt gcgtggcgcg ctgcgccagg atcccgatgt catcctcatt ggtgagatgc
     1081 gtgacgccga aaccgtccgt acggccatct cctcggccga gactggccac ctcgttttct
     1141 ccaccctgca cacggtcaac gcggtggaaa ctttgaaccg gatcatggac ttttttgaac
     1201 cgcaccagca gctccagatc cgaaaacagc ttgcgagcgt gctgcgggcc gtatgttccc
     1261 agcgcctgct cccgtccatc gaaggccacg gccgctgtgt cgccgcggaa atcctggtcg
     1321 gcaaccgcac ggtccgtgag tacattgaac agggcaagag cttcaacgac ctcactaaac
     1381 tgatcgaaga cggtttcgaa cagtacggca tgcagacctt cgatcaggcg ctgttcgtcc
     1441 tctggaaaga gaagcggatc gatgagcaga ccgcccttga taactccacc agcccacgtg
     1501 acctgaaact ccgcatggag gggttccgct gatcccctgg gcggaaacag cctgaaatcc
     1561 gccacagaac agccttttca aaggccaggt ccgcggacag ggtcaacggc acccggcaca
     1621 gactcgggcg gccggccaac gtatcgatgg cgaactttgc cgaccgtacg gttggcgggg
     1681 agccgctcga attgatcctg gccttcatgc tggagggcat gaattcgttg actctgcagc
     1741 gcccgccaac ctagactgct tccatggaaa cggtgagtat gctgctgctg gttttgtcgg
     1801 ccacgggggg caatcttctg ctgaacccgg gttttgaatc cgtgcgcgac aatgcccccc
     1861 tccgctggga tctgtacgtg atgcctcgcg ccggctgcca gggcgagctg gacagcagta
     1921 tcgcgcacga aggacagtac tcggtcaagc tgcacaacga gactccctac ggcaaagagc
     1981 ccgccaacaa ctggagtcaa agcatcacca ccaacctgcg caaccgcgaa ctccgcgtct
     2041 cgggatggct caaaacccag ggcgccaccg aggccgcgct ctggctgcag tgtttccaac
     2101 gcaacccgct gcgcatcctg gcgctggagt ccacgaccca cgctcagctc atcacgggcg
     2161 acaccgactg gaccgaggtc gagatgcggg tgaccgtgcc gaatgaaacc gatttcgtca
     2221 tgttccgctg cgtacttctg ggggaaggca ccgcatggtt tgacgacgtg tctgtcgagg
     2281 acgcccccga gcacatcagc accccacccg aagcagccgt tgccgaagcg gtgagaaagg
     2341 aagcgccgcc ccaggcgccg cccgcaagca aagactccgc ccagcttgaa gagctgcgga
     2401 tttcacgcca ggcgcttgcc gacgcaaacc ggacgctgcg cgagctgaat acgtcgcttt
     2461 tgacgcagat caccgagttg caggttgaaa tcgagcagct gcggcaggaa attcaagacc
     2521 tgcggctgca ggaagaggca gagcgcacag aggcgccgcc cactccctcc gtgccccaac
     2581 ccgaaatgcc tgtgccgcct ctcgtgccac acgttccacc cattgaaaag gagcagccat
     2641 gaacctggtg cttgtcatat ccattctggc cggtttgtcg gccgcgggac tggtctggtt
     2701 cggctggagc ctgctggcgg ccatcgcccc tcgttccagc ccggtttcga gcaacccggg
     2761 cggggatgaa cccggagacg atgaatgagg gactccgcag agggcgaacg caagccgccg
     2821 gcgaaaagga accgcccttt tcttggcgtg ctcttcacct gctgccatgt atatacccgc
     2881 atctacctca acccggccgg cacggcgttt accggtttct gccccaagtg cgggggcaaa
     2941 ctcaccgttc acgcccgcaa gggcggcagc aaatcgcgct tctggaccgc gggctgaacg
     3001 agggcacggt cttcaaccat agcgcagctg cccggccacg gagcagtggt ttccttcgta
     3061 cctacactgc cggatccaaa ccgcgcagtt tcaacagcat gttgatgccg tgaaggtctt
     3121 tattctgcgc atagtgcgcc cgtacggcct gcgaattgac ctcaggccag gttaatcccg
     3181 ccagttcatc cagcagggcc agatcggctt ccgtaagctc cccgcgcggc ttgtcgccca
     3241 gcaccgaacc atcccacggg aacagttcca ccttattcag cgtcatgatg tctttcgcca
     3301 cattgccgcg cacataatcc agcccccagc ggtgaagcat gccgcatttt ctgggatccc
     3361 gtgcaccatt ccggcattcc agccacacgc gtcccgatac ggcgtattcc tccgcgggca
     3421 ggtccagcgc atcgaaatcg atcttaaacg cctgccgctg caccgcgtcg agctgcgcat
     3481 ccgcgcagat ccagcgctcc cgggactcgt cccagtactc gcagagggtg tgatcgccga
     3541 aacggttgta ttttcggtgg aaatacgatt cgaatcccgt gcggagccgt gcggcccggc
     3601 cgtgagcgcg cagtatcgcg cacagaaaca gcgaataatc ccggcaggtg gccaccagcc
     3661 ggcgttccgg cgctcgtgcc accgtcagcg gcgcgggatc cagttcgaag aggcgctcca
     3721 gcatctcttc catcccgtag agatgcattt cctgcatgcg ctccggtgcg atttccacgc
     3781 catacttggc cgcgtgaaat acatggatta gcaatccttg aatgacctcg cacaacgcac
     3841 ccacctcatc cgggaggcgt tgcagcagct ccgcgtattc gcccggttct gaatgttcac
     3901 tgtgcgacgc gtagaacgtc ttcgattcca tgagcagggc catgtgtttc cttccggtgg
     3961 atttgtgcgc cggtttcctg gcggacgaga cgagttgcct gaattccgcc cagtttacta
     4021 aagtacgagc cggggtgcga ccctcatttt tgaaaggaca ggatatgctg ttcgctgtga
     4081 acctgctgtg ccttgccgcc gcgcaactga atctcctgac cgatccatcc tttgaggaag
     4141 cgctgaccca ttgggagcac ggcgctctcc acgcccgcat ggattccctg caggtggacg
     4201 gccgctccgt agcccggatt accgtccccg gcgacgcgcc cgtgggatgg ccctccattg
     4261 cccagcggat ccccgtgacg cccggcgacc tgctcgaagg cgccgccggc gttctggcgc
     4321 agggaatcca agacggcgcc ggggcatacc tcgccatcga attccatgat gccgccgggg
     4381 aacgcctcag ctatgcccag tctcgaggcc tgttgtgcga acccgaatgg caaaccctga
     4441 ccgtccgggc cgtcgccccc gggggcgcca ccgccgcccg cctctgcctg attctaaacg
     4501 gccggggaga ggccctgttc gacgacgccc gcctgacgcc cctcggcaat ctcacgcccg
     4561 aacctctcga aggcggagtc caaatcacgg ttacggacga ggtcgtctgc cccagcctga
     4621 ttgggtttgg cgcggaagac gacggctggt tctacgctcc cataaatctg gagcgcggca
     4681 tcacggcgga tgacatcgcg ctgcgggaaa aacggattga atggctcgac cccgactgga
     4741 tccgcatgtt cttctggtac agggattgga atcccagcgg cgactggaca tcattcacct
     4801 tcgacagccc caacatgcag agtcattacc gcaccctcga gctgtatcag cggatcggcg
     4861 ctgccgtcaa tgtgaccggc gtcgaatggc aattaaacga ccctttcggc catcccacgg
     4921 aaatgtctca cgccatcggg acgctgatgg agcatttgat caaaacccgg ggcttcacct
     4981 gtatacggca atggacgctc accaacgagc ccaactccca tttcattcag ctggggtacc
     5041 ccttcgaacg gtatgtcgag cttcaccgcc tcgtcaaagc tgaatttgcc cgccgggggc
     5101 tcgatgtcca aatcgtcgga agcgatgata ccgccggatt cgaatggttc caggcctgcg
     5161 tcaacaacga tgcgtatttt gacgcggccg acctgttcgc ttcacaccgc tatttcccgt
     5221 acgcggaccg ggtgctcgct cccttctttt tcgaagaccg ccaggcggcc ctggcgaagc
     5281 gcagcccggc gaaacctttc gtggtcgctg aattcgggtt tcaggatcac cgcgccggca
     5341 ccctcgccaa tccgctcatg gaagattatt cctatgccct ctggacgtat gcgttcatcg
     5401 tcgacggatt gaaccggggc gtcgcgggat tctccatctg gtgcatgcac gaggtttatt
     5461 atccggggaa cggcttcatg aactatgggt tgtggaacta ccgcgatcgc ggatggggca
     5521 tccggccggt gtatcacgcc ctgggctgtt ttacgcgcat gacccacgcg ggggatgccg
     5581 tgcgcaaatg cgtgtcctcg cacccggcgt atgtatcagc cgccgtggcg ggaaccaggg
     5641 tattctgggt gaatcaggct gacaacgaag tgtcggtggc ttgttcggcg ccactcacgg
     5701 gcccggccac ggcctacacc cgggactccg tcgccacgga cgactccccg gggatcgaca
     5761 taacggttga aggaaaccgg ttcgttgctc cgccccagag cttcggctat ctgtgtctcg
     5821 aagactagcc ggccgagcgc cggcgcagtt cggtcacaaa ttcttcgtca tcctcttccg
     5881 cacgacccat cggatgggct tccacgatcg gggcggccgc cggaaccggc acacccggca
     5941 caacgggttc agccggtcca gcgggtgcag ccgctgcggc cgctgccgcg ttggagccgg
     6001 ccttgctcac attgatcgct tgaagccctt tcgtgctgtg cagcaactca aagttgacaa
     6061 tatcgccttc tttcagcgtg cgaaaccctt ccatcttgat ggctgagtaa tgaacgaaaa
     6121 tatccggcgt gccgtcaatg acaataaacc cgtagccttt cgtgtcgttg aaccatttta
     6181 cccgcccttc gtgtatgccc gagagcgatt cattcgccat aacgccgacc tcccattcgg
     6241 gaatccccgg tgttatcccg cgcataatca gccccgcagg aaaaacctcc aggctaacgc
     6301 cacgtcgtcc acgcaccttc gcgcgcggcg attccctagt gctaccctat gtaacatgag
     6361 aaccttccca attgtttcgc aaccggccac gaatctgcgc ccgctggcag gcctgcggtt
     6421 gatcctggcg ccgcccggcg gcttttttgc cgtatcttca aacggggtca tcgaggcgcc
     6481 tggcccggcc agcttcggtt gatccggatg cgtatggctc gtatactttg gcccaacttg
     6541 gatttaggcg ggacccgccc ccgcggcgac ggggacgcac cgcgcaagat gacccacgga
     6601 gcggaccatg cgcattctcg tcaccggcgg cgcaggattt atcggctcgc acctgtgcga
     6661 agccctcgtg agccaagg
//
LOCUS       DAOPBQ010000154         6671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10203_length_6671_cov_3.806832, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000154 DAOPBQ010000000
VERSION     DAOPBQ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10203_length_6671_cov_3.806832"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..1803
                     /locus_tag="PKW60_06465"
     CDS             295..1803
                     /locus_tag="PKW60_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99111.1"
                     /translation="MRLLTSMLMSRWMPAVLVIACTALLAVVGLALPTNNDEGVWLYI
                     GKMWAQEGLRPYVDTFENKTPGIYYIFALASFFPGVGYFAGRVIALAVSAGSALLLVM
                     GLNRFHSRWAGIVGATCFSLANCWQFMQGALLNVTETYLIFFVFLAWYCLLQAWEKYR
                     QASAAMPWVLCAGVAMAAAINFKQIALTSWAAMALALLLYGAARKDWRGLFSALCAYG
                     AGTFLGFAVFTLPVLLAGVSLKAYWEGAWFILALPGTASDASMTQRLYVALVKWTGPE
                     MRLFLPFIGYLLCLFARDRHTRLLAAVLLIWLAFDFVGVNASGRYFGHHFKQILPPLS
                     VLVGYTSGEFIRRQFRDDDERRLAVGIAFLLVVALFFPANALVNGVSHLAFRKPDPAQ
                     EMGLWVRQHSTEGDYVQLAGGPISQVLPFTGRRAPGPYFHHCVLDLPGARDGFLRDAE
                     LHPPRYVLFQYESDLKPHPWLEDWLRQHYQPVYTDTGFRVYERSAGEASLTP"
     gene            complement(1831..>2654)
                     /locus_tag="PKW60_06470"
     CDS             complement(1831..>2654)
                     /locus_tag="PKW60_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99112.1"
                     /translation="FGPGQMWEIVADEIRGCGGEVLLRHKAVAITTEEGAVKAVTVRN
                     EDTGQEITHPADYVFSGMPIRELVEILDADTPASVRDVASGLMYRDFFTVGLLVEKMK
                     LEDPQTGGRIRDNWIYIQEPDVLLGRLQIFNNWSPYLVADPSKTWLGLEYFCYESDEI
                     WSRPDPELIELGTRELGQIGIIDPRDALDGCVIRVKKTYPAYFGTYTRFNEVRDYLDR
                     FENLFCVGRNGQHRYNNQDHSMLTAMLAVDNILAGVTSKKDIWEVNTEEEYHETK"
     assembly_gap    2655..2664
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2665..3399)
                     /locus_tag="PKW60_06475"
     CDS             complement(<2665..3399)
                     /locus_tag="PKW60_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPJ99113.1"
                     /translation="MQRKTAVIIGGGPAGLTAAYELVTRSDIQPLVIEQSSLVGGISR
                     TERYRGNHIDIGGHRFFSKSDRVMNWWLGIMPMQQSSEPAFAIQYQGQSRDVGLPHEG
                     LDPETTDRVMLIRPRKSRIYWRRKLFDYPLSFSVDTIRKMGLLRLGRAGLSYLHAAAF
                     PVAPEQNLEDFIINRFGRELYDTFFKSYTEKVWGVPCNEIPADWGAQRIKGLSILKAL
                     KHALMHRKGADVSQKGTETSLIERFLY"
     gene            3637..4143
                     /locus_tag="PKW60_06480"
     CDS             3637..4143
                     /locus_tag="PKW60_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99114.1"
                     /translation="MRDPLGSLTPSELLDNTTVDRENLIPNIHYLHDRGLVELMMGFS
                     APMFDGARITANGIDLVENTAEFGLRFPRQLDDEEALSAEVPILMERLVEEAELSALD
                     GEARRALLRDIQYLRDELARPLHRWRLPVIAAVLDWIDGHFTSSDECLPSFERFRTLI
                     RIHRTREG"
     gene            complement(4165..5022)
                     /locus_tag="PKW60_06485"
     CDS             complement(4165..5022)
                     /locus_tag="PKW60_06485"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPJ99115.1"
                     /translation="MNDPSPSITVEQIQALPTLPSVLTRILSTVADPESSALDLASHI
                     AADQALTATLLRIVNSAYYGFKREIAGIADAIVILGFIEVRNLALAATTFDVFPNSGS
                     GYDRVQLWRHSLAVAIASDRCARAARLVNPDTYFVAGLLHDIGKVVFDVLYPGRYPAA
                     VAHAQKQGLRVIEMERQCFGCDHTTLGAALSMHWNLPRLISASIREHHAAETDLTVPD
                     CAKIVALANCAASEAGLGQTRSGTVPLLSMDSTQALGISPEKLREIGVELRQAAPRID
                     ALLGVFPKE"
     gene            5318..5785
                     /gene="bcp"
                     /locus_tag="PKW60_06490"
     CDS             5318..5785
                     /gene="bcp"
                     /locus_tag="PKW60_06490"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HPJ99116.1"
                     /translation="MSKPEVGRNAPAFTLPASSGRHVKLSDFAGSPVVVYFYPKDDTP
                     GCTMEAREFQALAADFEKAGVSVLGISPDSVDSHCKFAEKHGLAFTLLADEKHDVAEK
                     YGVWVEKNMYGKKYWGVQRSTFLIDRNGAIAKMWPRVKPQGHAEEVLKAAREL"
     gene            complement(5892..6443)
                     /locus_tag="PKW60_06495"
     CDS             complement(5892..6443)
                     /locus_tag="PKW60_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99117.1"
                     /translation="MKRPALFLLIGCVSLAGCQTFIPAFTRLKPSYGELPTEALKESA
                     LKIERAIQDGATPELIDTPALTYNAAPIQQAIQTRVSRAALVNELLDKGHAWERRNGH
                     LYIIRNREYKRATTRRERDRNALIVTGEWDDRWMLYEQLLKINGFPQKSLSAIQSIFH
                     EARLEVMKTGQLYETAGGDPAPK"
     gene            complement(6440..>6671)
                     /locus_tag="PKW60_06500"
     CDS             complement(6440..>6671)
                     /locus_tag="PKW60_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPJ99118.1"
                     /translation="GARTQAQRLLEALRNHPYPGLPPNARVTVSIGVAVLDLDTMDNC
                     DALVAQADVALYEAKRLGKNRVVVAHTSGDKT"
BASE COUNT         1292 a         1936 c         2050 g         1383 t
ORIGIN      
        1 accaaatggc gcgacgatat acgccccggt aagattagca aatggcttga caggtacctt
       61 acaaatcgtg tcatccagca acggccggga atgacgctgc cttgtccatt gtaatcttga
      121 cggaacagag cgacggggca atgtgaggcc tcacgatcca ccgcgttaat ccaaacctta
      181 aaacgcgccg aattacagga aatacctgtg atttgaggcg actctctgca tgcccgtata
      241 ctttacgaca gaactcaggc ggttgcagcg gcacgttcca cccaggggaa acccatgaga
      301 ctcttgacat cgatgctgat gagccggtgg atgccggcgg tcctggtgat cgcatgtacg
      361 gccctgttgg cggtcgtggg gctcgcattg cccaccaaca acgacgaagg cgtctggctg
      421 tatattggaa agatgtgggc acaagagggg ctgcgcccct atgtagacac cttcgagaat
      481 aaaacccccg ggatttatta tatcttcgcg ctggcttcct ttttcccggg tgtgggctat
      541 tttgccgggc gggtgatcgc tctggccgtg agcgcaggct ctgcactgct gcttgtgatg
      601 ggactgaacc gctttcacag ccggtgggcg ggcatcgtgg gcgccacatg tttttcgctg
      661 gcgaactgct ggcagttcat gcagggcgcg ctgctcaacg tgacggagac gtatctcatc
      721 ttttttgtgt tcctggcatg gtattgcctg ctgcaggcct gggaaaaata ccggcaggcc
      781 agcgcggcca tgccctgggt actctgtgcc ggagttgcca tggcggccgc catcaacttc
      841 aaacagatcg ccctgacctc gtgggcggcc atggcactgg cgctgctgct ctacggggcc
      901 gccagaaaag actggcgcgg gttattttcc gcgctctgcg cctacggtgc gggcaccttc
      961 ctgggttttg cggtgttcac attgccggtg ctgctggctg gtgtctctct caaagcctac
     1021 tgggaagggg cctggttcat tctggcactg ccgggcacgg cgagcgatgc ctcgatgaca
     1081 cagcggcttt atgtcgcact ggtaaaatgg accgggccgg agatgcgcct gttcctgccg
     1141 ttcattgggt atctgctctg tctattcgcg cgcgatcgcc atacgcggtt gctggctgcc
     1201 gtgcttctga tttggctggc gtttgacttt gtgggagtca acgcctccgg ccgatatttc
     1261 gggcatcatt tcaagcagat tctccccccg ctcagcgttc tcgtgggcta cacctccggg
     1321 gagtttatcc ggcggcagtt tcgagatgac gatgagcgcc gcctggcggt tggaatcgca
     1381 tttctgctgg tagttgccct gttctttccc gccaacgcgc tcgtgaacgg tgtgagccat
     1441 ctcgcattca gaaaacccga tcccgcgcag gaaatggggc tgtgggtgcg gcaacactcc
     1501 accgagggcg attatgtgca gctggccgga ggcccgatct cccaggtcct gccgtttacg
     1561 gggcgccggg cacccggccc gtattttcac cactgcgttc ttgaccttcc aggcgcccgc
     1621 gacgggttcc tgcgcgatgc ggaactccat ccgccgcgct acgtcctgtt tcaatacgaa
     1681 tcggacctga aaccgcaccc ctggctcgaa gattggctgc gacagcacta ccagccggtc
     1741 tatacagaca ccggcttccg ggtctatgaa cggagtgccg gagaagccag tctcacgccc
     1801 tgacgaaacc ggttccatgc tgcccggagg ctacttcgtc tcgtggtatt cttcttccgt
     1861 gttgacttcc cagatgtcct tcttgctggt gacacccgcc aggatgttgt caaccgccag
     1921 catggcggtg agcatggaat ggtcctggtt gttgtagcgg tgctggccgt tgcgccccac
     1981 gcaaaagagg ttctcgaagc gatcgaggta atcacggacc tcgttgaagc gggtataggt
     2041 gccaaaatag gccgggtacg ttttcttcac gcgaataacg cagccgtcca gggcgtcgcg
     2101 aggatcgatg atcccgatct gtccgagttc gcgggtaccc agctcgatga gctcgggatc
     2161 cgggcgggac cagatctcgt cggattcgta gcagaaatat tcgagtccaa gccaggtttt
     2221 tgacggatcc gcaacaagat aggggctcca attgttaaag atctgcagac gcccgagaag
     2281 cacgtccggt tcctggatat agatccagtt gtcgcgaatg cggcctccgg tctgcggatc
     2341 ttcgagcttc attttctcga ccaggaggcc tacagtgaag aaatcgcggt acatgagccc
     2401 gctcgcgacg tcgcgcacgg atgcgggcgt atcggcgtcg aggatctcca ccagttcacg
     2461 tatgggcatg ccgctgaaaa cgtaatccgc gggatgcgtg atttcctggc cggtgtcctc
     2521 attgcgcacc gtgacggcct tcaccgcgcc ctcttcggtg gtgatggcaa cggccttgtg
     2581 gcgcaacagc acctctcccc cacacccgcg gatttcgtcg gcgacgatct cccacatctg
     2641 cccggggccg aattnnnnnn nnnnatagag aaaacgctcg atgaggctgg tttccgtgcc
     2701 tttctgggat acatcggcgc cctttcggtg catgagcgca tgtttgagcg ccttgagaat
     2761 ggacagtcct ttgatccgct gggcgcccca atccgcagga atctcattac acggcactcc
     2821 ccacactttt tcagtatagg atttgaagaa tgtatcgtac agttcacggc caaaccggtt
     2881 gatgatgaaa tcttcgaggt tttgctcggg ggcaaccggg aatgcggccg catggagata
     2941 gctcaatccc gcacgaccca ggcgcagcaa ccccatcttt cgaatcgtgt cgactgaaaa
     3001 gctgagagga taatcaaaaa gcttgcggcg ccagtagatg cgggacttac gggggcggat
     3061 gagcataacc cggtcggtgg tctcgggatc caggccctca tgggggagtc cgacatcgcg
     3121 ggactggccc tggtactgaa tagcaaaagc cggctcactg gattgttgca tgggcatgat
     3181 gcccagccac cagttcatca cacgatcgga cttgctgaaa aaacggtggc cgccaatatc
     3241 gatgtgattc ccacggtacc gttccgtgcg cgagatgccg cccaccaggc tggattgttc
     3301 aatgacaagc ggctggatat cggagcgcgt gaccaattca tatgccgccg tgaggccggc
     3361 cgggcctcct ccaataatca ccgcggtctt tcgctgcatt ccgcatcccc cgcgccggat
     3421 ggcgactcgg tttccttaca ttccgtgaca gcggcctccg acggcgaaga ttatgtccgt
     3481 ccggtgcata cacttcaagg acacccgccc tggaaagcac catccgggcg gccagactgc
     3541 catccggagg gttcgccctg ctataatccc ggcatggaca caccgcatcc caaacatgtg
     3601 cgacgcctgc ttttagaacg tttgtacgaa cgctatatgc gcgaccctct gggttccctg
     3661 acgccgtcgg agctcctgga caacacgacc gtggaccggg agaacctgat tccgaacatt
     3721 cactaccttc acgaccgggg cctggtcgaa ctgatgatgg gcttcagcgc gcccatgttt
     3781 gatggagcgc gtatcaccgc aaacggcatc gatctggtcg aaaatacagc cgagttcggg
     3841 ctgcgctttc cccggcagct ggacgatgaa gaggcgctct cggctgaagt gcccatcctg
     3901 atggaacgtc tcgtcgaaga agccgaattg agcgccctgg atggcgaagc acggcgcgcg
     3961 ctgctccgcg acatccagta cctgcgcgac gaactcgccc gcccgctgca ccggtggcgc
     4021 ttgccggtca ttgccgcggt ccttgactgg atcgatgggc attttacttc cagcgacgaa
     4081 tgcctgcctt cgtttgaacg gttccgcacc ttgatccgga tacaccggac gcgtgaaggc
     4141 tgaggagcgc ggcggtaacc gcccctactc tttcgggaag acaccgagga gcgcgtcgat
     4201 gcgcggagca gcctgacgca attcgacccc gatctcacgg agtttctccg gggaaatccc
     4261 cagagcttga gtcgaatcca tcgaaagcaa gggcaccgtg ccgcttcggg tttgacccag
     4321 tcccgcctcg ctggcggcgc aattggccag agccacgatt ttcgcgcaat cggggaccgt
     4381 taggtctgtt tccgctgcgt gatgctcgcg aatgctggcg ctgatcaggc gggggaggtt
     4441 ccaatgcatg ctgagcgcgg cgccgagcgt ggtgtggtcg cagccgaaac actggcgctc
     4501 catttcaatg acccgcagcc cctgcttttg agcatgcgcc acggcggcgg gatagcgccc
     4561 ggggtacagc acatcaaaga cgaccttgcc gatatcatgc aggagcccgg caacaaaata
     4621 ggtatcgggg ttgaccaggc gggccgcgcg cgcgcaacgg tcagatgcga tggccaccgc
     4681 gagcgaatga cgccagagct ggacgcgatc atagccggat ccggagttcg gaaagacgtc
     4741 aaacgtggtt gcagcgagtg cgagattacg cacttcaatg aaacccagaa tcacaatagc
     4801 atcagcgatg cccgcgatct cacgtttgaa cccgtagtac gccgaattga cgatgcgcag
     4861 cagtgtggcg gtcaacgcct gatcggcggc gatgtggctg gccaggtcca gcgcggacga
     4921 ttcggggtcg gccacggtac tcagaatgcg cgtcaagacc gacggaagcg tcggcaacgc
     4981 ctgaatctgc tcgacagtga tcgaagggga tggatcattc acaacgatgc ggcccctacc
     5041 cgtaaaccca cgcagctgac aggaagcata ccggtttggg cgcgcgaggc gcaacgcgcc
     5101 cgccgcgtgg agcaagcctt ccgcacactg ttttcttata cccgcacggg ccgcgtacag
     5161 gcgggcgtgc actgcccgta ttctctgtct gttgtacggc tggacctctc cgattcatga
     5221 cgcgtcattt cgggggatgt ttcgatgggc ttttcgtgtt agcctacagg gaagacaatg
     5281 gttgcggagc cgccccaggc cttcaaggag aagccgtatg tcgaagcctg aagtcgggag
     5341 aaacgcgcca gctttcacgc tgccggcgag cagcgggcgc cacgtgaaat tatcagattt
     5401 tgcaggaagc ccggtggtcg tctatttcta tccgaaggac gacacgcccg gatgtacaat
     5461 ggaagccagg gagtttcagg ccttggcggc ggatttcgaa aaggccgggg tgtctgtact
     5521 gggaatcagc cccgacagcg tcgattcaca ctgcaagttc gccgaaaaac acgggctcgc
     5581 gttcacattg ctggcggatg agaaacacga cgtcgcggag aaatacggcg tctgggttga
     5641 aaagaacatg tatggcaaga agtactgggg cgttcaacgc agcacatttc ttatcgacag
     5701 gaacggcgcc atcgcgaaaa tgtggcccag ggtgaagccg cagggccacg ccgaagaggt
     5761 gctgaaagcg gcccgggaac tgtaggccgc atgtcctgcc aggcaggggt gtcgaggcct
     5821 ccctgctggc aggggcaacc tcgcccgggg tggaagcccg tcaagcgccc ttccgcgtca
     5881 gcgaatggtc atcatttcgg ggcggggtcg ccgccggccg tctcgtacag ctggcctgtc
     5941 ttcatgacct cgagccgggc ttcatggaag atgctttgaa tggccgagag agacttctga
     6001 gggaagccat tgatcttcag cagttgctcg taaagcatcc agcggtcgtc ccattcgccg
     6061 gtgacaatca gggcgtttcg gtcgcgttcc cggcgcgtgg tggcccgttt atattcgcgg
     6121 ttgcgaatga tgtagaggtg gccgtttcgc cgttcccagg catgcccctt gtcgaggagt
     6181 tcattgacga gcgccgctcg ggacacacga gtttgaatag cctgctggat gggagctgca
     6241 ttataggtca acgcgggggt gtcgatgagt tcgggggtgg cgccgtcctg gatggctcgc
     6301 tcgattttga gagcgctttc cttgagcgcc tccgtaggca gttccccata ggacggcttc
     6361 aggcgcgtga aggcggggat gaacgtctgg cagcctgcaa gcgagacgca cccaatcaac
     6421 aaaaacaggg cgggacgttt cacgttttat ctcccgaagt gtgtgcgacg accacccggt
     6481 tttttccgag gcgtttggcc tcgtacaggg cgacatcggc ctgcgccacc agggcgtcgc
     6541 agttgtccat cgtgtccaag tcgaggacgg ccacgccaat acttacggtg actctggcgt
     6601 tgggaggcag tccgggatac gggtggtttc ggagggcctc gagcagacgc tgtgcctggg
     6661 tgcgcgcgcc c
//
LOCUS       DAOPBQ010000155         6669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10208_length_6669_cov_4.640157, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000155 DAOPBQ010000000
VERSION     DAOPBQ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10208_length_6669_cov_4.640157"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /gene="nrdD"
                     /locus_tag="PKW60_06505"
     CDS             <1..1289
                     /gene="nrdD"
                     /locus_tag="PKW60_06505"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPJ99119.1"
                     /translation="APPQDLESLLVQLGRFRTQVDAHFAGPVSWDMPHVYLSPFLGNT
                     TAQERQRAAQILLFVLGTDATQRAAKPELTLSWDVPARLARRKAAGPAQVVEGRVYAD
                     FEPLAQQLMDDILAIYLQANATNLPLLRFVLDRSTFARRGCRNILVRLAETALASGLL
                     EIEFDRPCDDAACPPFRDGSPVAHVVNLNLARMAFRSTNETALMENLDALVDLAAQAH
                     QEKCAFLERLAALRALGPLVFLAESPAHPPLLDISKAVYAIAVDGLSECIEALCGAAP
                     HQSGDALALAVRVLTRLAEGCRLAGDRTGLRIVPAQARYESSVRRFAALDLRPFPEEA
                     RRVAKSDPITQDIHYTAGARVARGAACTPSERALLEGEFQALVSEGALARIELPDMET
                     SPEAVASFIEMAWRRTKIRHLLLERRGAPTGFLPHD"
     gene            complement(1386..2336)
                     /locus_tag="PKW60_06510"
     CDS             complement(1386..2336)
                     /locus_tag="PKW60_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HPJ99120.1"
                     /translation="MANETVFRRCEHNPIVTPAAVPTANSIFNSAVVPFGTGFAGVFR
                     VDSQRMEAGLHAGFSDDGLAWQIEASPILFERNELDGPDVNYDGRFQYDPRITRIDDV
                     YCVTWCHYPDAPQGGQAPAIGLAQTQDFHRFELIDANVAYLNRNGVLFPRKIGGMYAM
                     LHRPSDLGHTPYGDIFYSTSPDLVHWGRHRFVFGPRGGWQSTKVGAGPVPIETDEGWL
                     LIYHGVRTSCSGFVYSVGAALLDLEKPWQVRYRTRTYLLAPAEPYERVGDVPSVVFPC
                     AALVDSQRQVSLYYGAADTVLAVAYANLDDLIAFTKENSF"
     gene            complement(2429..3613)
                     /locus_tag="PKW60_06515"
     CDS             complement(2429..3613)
                     /locus_tag="PKW60_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPJ99121.1"
                     /translation="METLKLLVCDDEMGMRMGVARALRDFSVDIPDMDESVRFQVELA
                     ETGEEALEKITAEPPQILLLDLKLPGISGLDVLEELHKRDVETLTVMITAYASIETAV
                     RATKQGAYDFLAKPFTPTELKSTVTKAAMHLMVARQARRLAQEKRQVRFQFISVLAHE
                     LKAPINAIEGYLNVVRDGTLGDNPQVINQIIFRCLDRIQGMRKMIGDLLDLTRIESGQ
                     KRREMERVDAGSMALRAIETFLPEAQSRNITIDLHRTGDLVLWADTGELEIILNNLVS
                     NAVKYNRDDGRVDVTLSRHEDFLHIAVKDTGIGMTRDEAAKLFNDFVRIKNSSTSGIL
                     GSGLGLSIVKKLALLYEGKVKVDSEPGEGSTFTVTLKDVTPADTPAGAQAAEPQPAAD
                     AQ"
     gene            complement(3731..4123)
                     /locus_tag="PKW60_06520"
     CDS             complement(3731..4123)
                     /locus_tag="PKW60_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPJ99122.1"
                     /translation="MSDEKKMILVVDDDEDFLFQQKVQLEAAGYKVLTAAGLSAARSL
                     LRSTRPDMAVIDLMMEEMDAGFTLCREIKKTYPGCPVVICTGVANETGMAFDAATEEE
                     RAWVKADALLDKPVRFEQLQREIERLIR"
     gene            complement(4260..6326)
                     /locus_tag="PKW60_06525"
     CDS             complement(4260..6326)
                     /locus_tag="PKW60_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPJ99123.1"
                     /translation="MKSASNIPLVTTIKERCRVCFTCVRECPAKAIRIVDGQADVICN
                     RCIGCGNCVRVCSQGAKRVLDSKAEVEALLAGEARVAALLAPSFPVEFDDCEYERVVG
                     MLRALGFDSVHEVAFGADLVADRYRRLVETTQDKRFVATTCPSIVAYVQRYQPDLVTS
                     LAPIVSPMVAMARVLHQRHGPGLKIVFIGPCIAKKGEAASDELPDDVHSVLTFAELQA
                     LLTARNITPESVEPSDFDPPHARYGGLFPVSRGLLQAANIVEDLVDGNVAVADGRVDF
                     PEAISEFAAGFFEVRLLETLACKGCIMGPGTTNQDPQYVRRCRVSSYVRRRMKNTVEA
                     EWADAMQEFSTLDLGRSYRPDDQRIAVPSENQIREIMERMGKFSPGDELNCGACGYET
                     CREHAIAIYKGLAETAMCLPHTIELLATTIQDLNQSNEQLAEARSALVQSEKLASMGQ
                     LAAGIAHELNNPLGIVLMYAHILKDEFGENVQMLEDLKMISEHADRCKKIVAGLLHFA
                     RQNKVLLESVDVGDMLEHVLRANTVPENVRVRFENEMSDPRADIDRDQMTQVLTNLVG
                     NALAAMPDGGVLTVRLTDEADRIMLQILDTGTGIKKENLSRIFDPFFTTKQLGVGTGL
                     GLAVTYGIVKMHRGDIRVESNSDPAKGQTGTTFKISLPRHSQAGRPESAEMSIGLGGE
                     EELLGA"
     gene            complement(6335..>6669)
                     /locus_tag="PKW60_06530"
     CDS             complement(6335..>6669)
                     /locus_tag="PKW60_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HPJ99124.1"
                     /translation="EIGVAVVSGLQNARLLLEEIRNGRDDIHFIEVMTCPGGCIAGGG
                     QPLNADPDAIRARMQALYTIDRDEPVRTSHGNPAIQRLYAEYLGEPLGEKSHQLLHTH
                     YAKREVMI"
BASE COUNT         1159 a         2226 c         1945 g         1339 t
ORIGIN      
        1 ccgctccgcc tcaagacctg gaaagcctgc tggttcagct gggccggttc cgcacgcagg
       61 tcgacgccca ttttgccgga ccggtctcct gggatatgcc ccacgtatac ctcagtccgt
      121 ttctgggcaa caccaccgcc caggaacgcc agcgggcggc tcaaatcctg ctctttgtgc
      181 tcgggacgga cgccacgcaa cgcgcggcca agcccgaact cacgctgagc tgggatgtcc
      241 ccgcgcggct ggcgcgccga aaagccgcgg ggccggccca ggtcgtcgaa ggccgggtct
      301 acgcggattt cgaaccgctc gcccaacaac tcatggacga cattttggcc atctacctgc
      361 aggccaacgc cacgaacctg cccctgctga ggttcgtcct ggatcgttcc acgtttgcac
      421 ggcgcggctg ccgcaacatc cttgtacgcc tggccgaaac cgccctggcc tccggactcc
      481 tcgagatcga gttcgaccgc ccgtgcgatg atgccgcgtg cccgcctttc cgtgacggca
      541 gccctgtcgc tcacgtggtc aatctcaatc tggcgcgcat ggcgtttcgt tccacaaacg
      601 aaaccgccct gatggaaaac ctcgacgccc tggttgatct ggccgctcag gcgcaccagg
      661 agaagtgcgc gtttcttgaa cgcctcgccg cgctgcgcgc gctcggcccc ctggtgtttc
      721 tggcggagtc gcccgctcat ccgcccctgc tcgatatctc caaggcggtg tatgccattg
      781 ccgtggacgg actcagcgag tgcatcgaag cgctctgtgg agcagcgccg caccagtccg
      841 gcgatgccct ggcgctggcg gttcgagtgc tgacccggct cgcggaggga tgccggctcg
      901 ctggcgaccg caccggcctg cgcatcgtgc ccgcccaggc ccggtatgag tcctcagtcc
      961 ggcgtttcgc ggcgctcgat ctccggccct tccccgagga ggcccggcgc gttgcgaaga
     1021 gcgatcccat cacccaggac atccactata cggcgggggc ccgtgtcgcc cggggtgccg
     1081 cttgcactcc gagcgagcgc gccctgctgg aaggggaatt ccaggcgctt gtgtctgaag
     1141 gcgcattggc gcgcatcgaa ttgcccgaca tggagaccag tcctgaggcc gtcgccagtt
     1201 ttattgagat ggcctggcgc cgcacgaaaa tacgccacct gctcctggaa cgccgcggcg
     1261 cgcccaccgg cttcctgcca cacgactgac ctgagccggg gccgttcgcg tcacagcagc
     1321 cccgccaatg cctgctccgc cacggctggc cgccggcaga ggagctgctc gcgcggaacg
     1381 cccggttaga agctgttttc ttttgtaaag gcgatgaggt cgtccagatt cgcgtaggcc
     1441 accgccagca cggtgtccgc cgcgccgtaa tacaggctga cctgccgctg gctgtccaca
     1501 agggccgcgc acggaaatac cacgctcggc acgtcgccca cgcgctcgta gggttctgca
     1561 ggcgccagga ggtaggtgcg ggtgcggtag cggacctgcc acggtttttc caggtcgagc
     1621 agtgctgcgc ccacactgta gacaaacccg gagcagctcg tgcgcacgcc gtgataaatc
     1681 agcagccagc cttcgtccgt ctcgataggc acggggccgg cccctacctt tgtgctctgc
     1741 catccgccac gcggaccaaa cacgaagcgg tgacggcccc aatgcacgag atccgggctc
     1801 gtcgagtaaa agatgtcgcc gtacggtgta tgcccgagat cgctgggacg gtgcagcatg
     1861 gcgtacatcc cgccgatctt ccgtggaaaa aggactccgt tgcggttcaa ataggccaca
     1921 tttgcatcga ttagctcgaa ccggtgaaaa tcctgggttt gggccaggcc gatagcgggc
     1981 gcctgcccgc cctggggcgc gtcggggtaa tggcaccagg taacgcagta gacatcgtca
     2041 atccgtgtaa tccgggggtc gtactgaaac cggccgtcgt agttcacgtc cggcccgtcc
     2101 agttcattgc gttcaaacag aatgggcgat gcctcgatct gccaggccag tccgtcatcg
     2161 ctgaaccccg cgtggagccc ggcctccatc cgctgggaat ccacccggaa cacgccggca
     2221 aacccggttc caaacggaac caccgcgctg ttgaaaatag agttggcggt cggcacggcc
     2281 gccggggtga cgatcggatt gtgctcgcag cgccggaaca cggtctcgtt ggccatgaaa
     2341 ttctccttga cgccccagca cccggatgca ggtcagcccc accgtccctc acagcctgat
     2401 gcgcgaaccc cccggatgcc tgcccgggct attgcgcatc agccgccggt tgcggttctg
     2461 ccgcctgcgc cccggcgggc gtgtctgccg gtgtgacgtc tttcagcgtc accgtaaagg
     2521 tggaaccttc gccgggttca ctgtcaacct tgaccttgcc ctcgtacagc agcgccagct
     2581 ttttcacgat cgacaagccg aggccgctgc ccaggatgcc cgaggtgctt gagtttttga
     2641 tccgcacgaa atcattgaac agtttcgccg cttcgtcccg ggtcattccg atgccggtgt
     2701 ctttcacggc gatgtggaga aagtcttcgt ggcgggagag ggtaacgtcg acccgtccat
     2761 cgtctctatt gtatttaacg gcattcgaaa ccaggttgtt caggatgatc tcgagttcgc
     2821 ccgtgtccgc ccacaacacc agatcgccgg ttcgatgcag gtcgatagtg atgttgcggg
     2881 actgggcttc ggggagaaag gtctcgatcg cgcgcagcgc catgcttccg gcgtccacac
     2941 gttccatctc acgccgtttc tggccggatt caatgcgcgt caggtccagc agatcgccaa
     3001 tcatcttccg catcccctgg atccggtcca ggcagcggaa aatgatctgg ttaatgacct
     3061 gtgggttatc gccgagggtg ccgtcgcgga cgacgttcaa ataaccttcg atggcgttaa
     3121 tcggcgcttt gagttcatgt gcaagtacgg aaatgaactg aaaacgcacc tggcgttttt
     3181 cctgggccag ccggcgcgcc tggcgcgcca ccatcagatg catggccgct ttcgtaaccg
     3241 tgcttttcag ttctgtcggg gtgaagggct ttgccagaaa atcgtaggcc ccctgttttg
     3301 tggcgcgcac cgcggtttcg atcgaagcgt aggccgtgat catcacggtc aaggtttcga
     3361 cgtcccgttt gtgcagttcc tccagcacgt ccaggccgct gatgccgggg agtttcaggt
     3421 cgagcagcag gatctggggc ggctccgctg taattttctc aagcgcttct tcgccggtct
     3481 ccgccagttc cacctggaac cgcaccgact cgtccatgtc agggatgtcc acgctgaaat
     3541 cgcgcagggc acgcgcaacc cccatgcgca tgcccatttc gtcgtcacaa accagcagct
     3601 tcagcgtttc catagtactc ccttcggccg gtggcgtcga ttgtccgtcc aggccgcgtg
     3661 gcgcatggac ggccgccacc caacacccgc tctgctctgg tacgcgctca gtcggcgcca
     3721 ggcggcccaa tcagcgaatc agccgttcga tttcgcgttg aagctgttca aagcggaccg
     3781 gcttgtccag cagcgcatcg gctttgaccc aggcgcgctc ctcttccgtc gccgcgtcga
     3841 aggccatacc tgtctcgttg gccactcccg tgcagattac cacagggcat ccgggatagg
     3901 tttttttgat ctcccggcac agggtaaagc ccgcatccat ttcttccatc atcaggtcga
     3961 tcacggccat gtccggcctg gtggaacgca agagactgcg tgccgcgctc aaccccgcag
     4021 cggtgagcac tttgtatccc gccgcttcaa gctgcacttt ctgctgaaac aggaagtctt
     4081 cgtcgtcatc tactaccagg atcatctttt tctcgtcgct cattacatca tcccatctat
     4141 gggaactgcc ggcctcggcg ccagcggttt gcccgtcatt cgcattaacg caccaaacac
     4201 cttacgatac cgcaatctgg gttgggaatg aaactttcgt tccttttccg catcggcggc
     4261 taggcgccca gcagctcttc ttcgcccccc aggccgatgc tcatctccgc tgattccggc
     4321 cggccggcct ggctatgccg gggcaacgag atcttgaatg tcgtgcctgt ctgccccttg
     4381 gccgggtccg aattcgattc cacgcggatg tctccgcggt gcattttaac gatgccgtac
     4441 gtcaccgcca ggcccagtcc cgtgccaaca ccgagttgtt tcgtcgtgaa aaacggatcg
     4501 aaaatccgcg agaggttctc ttttttgatg cccgtgcccg tatcgaggat ttgcagcata
     4561 atccggtcgg cttcgtccgt cagccgcacc gtcaacacgc cgccgtcggg catggccgcg
     4621 agcgcattgc caaccagatt ggtcagcacc tgcgtcatct gatcgcgatc gatatccgcc
     4681 ctgggatcgc tcatttcgtt ctcaaagcgg acccgcacat tttcgggcac cgtgttcgcc
     4741 cggagcacgt gttcgagcat gtctccaacg tcgaccgact cgagcaagac tttgttctgc
     4801 cgcgcgaagt gcaagagccc cgccacgatc ttcttgcagc ggtcggcatg ctccgagatc
     4861 atcttgagat cttccagcat ttgcacgttt tcgccgaact catccttgag gatatgggcg
     4921 tacatcagca cgatgcccag cgggttattc aattcgtgtg cgatacccgc cgccaactgg
     4981 cccatgctgg ccagtttttc cgactgcacg agtgccgagc gcgcttccgc gagctgttcg
     5041 ttcgactgat tgagatcctg aatggtcgtt gccagcagct caatcgtgtg cggaaggcac
     5101 atcgcagtct ccgccagccc tttgtagatc gcaatggcgt gttcgcggca cgtctcgtag
     5161 ccacaggcgc cgcaattgag ctcgtcgccc ggtgaaaact tgcccatccg ctccatgatc
     5221 tcgcggatct gattctccga cggcaccgcg atccgctggt cgtccggtct gtacgaccgg
     5281 cccaggtcca gcgtgctgaa ttcctgcatc gcgtccgccc actccgcctc tacagtgttt
     5341 ttcatgcggc ggcgcacata cgagctcacc cggcaacgcc ggacgtattg cgggtcctgg
     5401 ttcgtcgtcc ccggccccat gatgcagcct ttgcaggcca gagtctccag cagacgcacc
     5461 tcaaaaaatc ccgccgcaaa ttcgctgatc gcctcaggaa aatcgacgcg gccgtccgcc
     5521 accgccacat tgccatcaac gaggtcctcg acgatattcg ccgcctgcag gaggccccgg
     5581 ctgaccggaa acaggccgcc gtagcgcgcg tgtggcgggt caaaatcgct cggttcgacc
     5641 gattcaggcg tgatgttccg tgccgtcagc agcgcctgca attctgcgaa agtcaggacg
     5701 ctgtgtacgt catccggcag ttcgtcgctc gccgcctcgc cttttttcgc gatacacggg
     5761 ccgatgaaca cgattttgag cccaggccca tgccgctgat gcagcacgcg cgccatggcc
     5821 accattggcg aaacgatggg cgccagcgag gtaaccaggt ccggctggta ccgctgcacg
     5881 tacgccacaa tcgacgggca cgtcgtcgcc acaaaccgct tgtcctgcgt cgtttcgacc
     5941 agtctgcggt accggtccgc aaccaggtcg gcgccaaagg caacctcgtg gacagagtcg
     6001 aagcccagcg cccgcaacat gcccacgacg cgctcgtatt cgcagtcgtc gaactccacg
     6061 ggaaaactcg gcgcgagcag cgccgccacc cgggcctctc cagccagcag cgcctcgacc
     6121 tccgccttgg aatccagcac gcgcttggcg ccctggctgc acacgcgaac acagttgccg
     6181 caaccaatgc agcggttgca gatcacatcc gcctggccgt caacgatgcg gatggccttc
     6241 gcgggacatt cccgcacgca ggtgaaacag acgcggcacc gttccttgat ggtggtgacc
     6301 agcggtatgt tggatgcgct cttcatggcc ggcctcagat cataacctcg cgcttggcgt
     6361 agtgcgtgtg gagcagttga tggctttttt cgccgagcgg ctcgcccaga tactctgcat
     6421 acagccgctg aatggcgggg ttgccgtgcg aggtgcgcac gggctcgtcc cggtcaatcg
     6481 tatacagggc ctgcattcgc gcccggatgg cgtccgggtc cgcgttaagg ggttgtccgc
     6541 cgcctgcgat acagccgccg gggcaggtca tcacttcaat gaagtgaatg tcgtcgcgcc
     6601 cgttgcggat ttcctcaagc aacagccgtg cgttttgcag gccgctgacc accgccacgc
     6661 caatctcgg
//
LOCUS       DAOPBQ010000156         6647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10244_length_6647_cov_3.731341, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000156 DAOPBQ010000000
VERSION     DAOPBQ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10244_length_6647_cov_3.731341"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..960)
                     /gene="truB"
                     /locus_tag="PKW60_06535"
     CDS             complement(67..960)
                     /gene="truB"
                     /locus_tag="PKW60_06535"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPJ99125.1"
                     /translation="MNGLLLVDKPAGVTSHDVVRHVRRTAASRKVGHTGTLDPAATGL
                     LVLCLGQATRISDFLIGMDKTYEGVLRLGLVTSSHDLDGEILEERPVPSLTSDDLEQA
                     FAAFTGEIQQVPPMVSAVKIGGERLYKRAWRGENIDRPARPVTVHEFALRSFDGRDAA
                     FRLRCSTGTYARVLCFDAGTRLGCGGVLASLRRLSVGSYSVADAATLDSLDSRDAVAK
                     RLLPLETALSLPRVEVQPMGLNLVRHGRPLDRDALTGDCPVHEGWIQILAPSGELLAL
                     GEVSADALGVRILPRRVLCHE"
     gene            complement(957..1937)
                     /locus_tag="PKW60_06540"
     CDS             complement(957..1937)
                     /locus_tag="PKW60_06540"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPJ99126.1"
                     /translation="MPQSKLAEILQRIRQAKRILVTSHVSPDGDAVGAVLAVYHIIRT
                     LHDPEVVCSLAHPVPKRYAWLPGAERICPPEVCAPPFDLAVIVDVAQLDRIGEVSDLI
                     DENTEVIVLDHHLEDDPIGDLAVVDPRYAATGELIIDLMDCAGVSLTREIAECAYVAL
                     ATDTGSFKYSNTTERTHRIAARLVATGLPVAEIGRRVFDEMSPARFRLLNRFVNSVTL
                     LDGGRIAIGTVTAADMAESEALEEDTEGLINFARNIEGVEVALLFREVDSHTVKLSLR
                     SREGVNSAEIVRPLGGGGHAAAAGVTLELPLRQATAVALRHVREALQDTL"
     gene            complement(1924..2295)
                     /gene="rbfA"
                     /locus_tag="PKW60_06545"
     CDS             complement(1924..2295)
                     /gene="rbfA"
                     /locus_tag="PKW60_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPJ99127.1"
                     /translation="MSHGREKRVGELVRQEIAHLLTHGLKDPRIGFVSVMHVRMSPDL
                     RYANAYVSLMGESKQRKASLAALRRSATWLRGEVGRRLRMRHAPEIRFFEDTSVDDAF
                     RLEEIFKEIHAEEQDDRDAAE"
     gene            complement(2299..2580)
                     /locus_tag="PKW60_06550"
     CDS             complement(2299..2580)
                     /locus_tag="PKW60_06550"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HPJ99128.1"
                     /translation="MTIGLLTLDLLIHGARSLKDKRRVVKSLKDRIRNRYNASVAEVD
                     FLDEWRRARLAVCVVARESRFANTQLNEIGRYAATCSEAEVTNIAIEML"
     gene            complement(2618..5422)
                     /gene="infB"
                     /locus_tag="PKW60_06555"
     CDS             complement(2618..5422)
                     /gene="infB"
                     /locus_tag="PKW60_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPJ99129.1"
                     /translation="MQTVASLAKRLDMTAEEALEKLRFMLMEVESVDSELTDDQCDLL
                     IDVDDDPSVAERVRQKKLDEEEKARKRTERLQAAAKKAAAKRKLAAKKKPAAKKTPTK
                     AKAASTKAAAKKKKSEDEPEESPLVEILRPEAEEEAPAAAAEAAGKAETKKAAPKKAA
                     AKKPARTTEPDILIGRAVDHEDVVQIIRADGTRVKELDAVLEETPVFEDDEAELESAI
                     LAEAERHQEEEEARKAREAARAMVKPDPAVVAEVKRRAEERMARKRQQRAELAATVTT
                     AATAAPATKRTVTGKTARKRQKRVERQRAEEAMRRDAAAKVREFQAAGPTGQIKKRRK
                     KRDKDDELGSAVDEIFEKHIIEVDETMTVEQLAEALDMATSDVILELMEHNILANKNQ
                     SLSFDLIRQIGEAHGYEVRSAIPGEEEIMAEEPDAPEDLVLRPPVVTVMGHVDHGKTT
                     LLDYLRRAHVAEGEVGGITQHIAAYDVEIRGGRVVFLDTPGHAAFTQMRARGAQATDV
                     VVLVIAADDGIMPQTVEAIDHARAAEVPIVVAINKCDKPEAQPDRIRQELVKYDLVDE
                     QWGGKTIIRNISAKTGEGVDTLMEMLVLEAEMLELRANPRKKARGAVVESELSKGQGP
                     VAWVLVQTGTLHVGDVFLAGETYGRVRAMINSRGEQVENVPPATPVLVLGFNDVATAG
                     DQFVVVDDERIARAIAEKRIDRAKLKQGATARRITLEDFHKHLKEGERHVLNVVIKAD
                     VQGSVDVLESNLLKLGNEDVKVELVHSGVGGINESDVILASASDAVIIGFHVTANPRV
                     RKLAEQEGVDIRTYRIIYEVIDEVKKALEGLLTPETKEVVTGHVEIREIFRSSAIGNI
                     AGCYVQDGEISRASLIRVVRNDVVLYEGRIASLRREKDDVRSVATGFECGIVLESFQD
                     VKVGDIIEAYRLEQVAQKLE"
     gene            complement(5480..>6647)
                     /gene="nusA"
                     /locus_tag="PKW60_06560"
     CDS             complement(5480..>6647)
                     /gene="nusA"
                     /locus_tag="PKW60_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003231912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPJ99130.1"
                     /translation="QQLEAEKSIDRDKLLEAIRTALESAARKSFHPKAEIVVEVDPAS
                     LVFKVYEIREVVESVTDDTREMTVEEAQGMNPEAGVGDRLKVPAEPKDFGRIAAQTAK
                     QVIIQKLKDAERENVYDEFKQREGEMVTGVVKRISHGNIIVSIGRADAVLPLREQSPR
                     ENFKPGDRIRAFLVEVEKSPRGPQVVLSRACPDLVRALFELEVPEIYDGTVEIKGIAR
                     EAGNRTKIAVHSNDLNVDPVGACVGMKGSRVRAVVEELCGEKIDIVRWSEDPVEMSTN
                     ALNPADILDISVDETNRVILVVVPHDQLSLAIGKRGQNARLASKLMGWNIDIKSDVEL
                     TGGAAPDAGSTGAPPEDAPPLNTDETADVDEDEPLKEEADADGPASNEPTRGDL"
BASE COUNT         1146 a         2120 c         1932 g         1449 t
ORIGIN      
        1 aggacgacgc cgggataaac ggcatccgca gttcgaacat cttcgataat cttcatttgc
       61 tcgtagtcac tcatggcaca acaccctgcg cgggagtatc cgaacgccga gcgcgtcggc
      121 agagacttct cccagggcca gcagctcccc ggacggcgcg agaatctgga tccacccttc
      181 atgtacaggg caatcgccag tgagcgcatc gcggtcgaga gggcggccat gtctcaccag
      241 gttcaacccc attggctgga cctctacccg gggcaaggac agcgccgtct ccagggggag
      301 caatctcttt gccaccgcat cacgggagtc gagggaatcc agggttgcgg catcggccac
      361 cgaatagctt cccaccgaaa ggcgccgcag actggccagg acgccgccac aaccgaggcg
      421 cgtacccgca tcgaaacaca agacacgggc ataggtcccg gtcgaacagc gcagccgaaa
      481 tgccgcatcg cgtccatcga acgagcgcag ggcaaattca tgcacagtga cgggccgggc
      541 cggccgatcg atgttttcgc cgcgccatgc acgcttgtag agccgctctc cgccaatctt
      601 cacagcgctc accatgggcg gaacctgttg aatttcgccc gtgaacgccg caaaggcctg
      661 ttccagatca tcggatgtca acgaaggaac cggccgctct tccagtattt caccatcgag
      721 gtcgtgcgac gacgtcacga gcccgagccg cagcacccct tcatacgtct tgtccatccc
      781 gatcagaaaa tcggaaatcc gggtggcctg cccgagacag agaaccagga ggccggtagc
      841 ggccggatca agcgtacccg tatggcctac ctttcgcgag gccgcggtgc gccggacgtg
      901 ccggactacg tcatgcgaag tgacccccgc aggcttgtcc acgagcagca agccgttcat
      961 agcgtatcct gcagggcttc tcgaacgtgg cgcagggcga cggcagtggc ctggcgcaag
     1021 ggcaattcga gggtgacgcc ggccgccgcg gcgtggccgc caccaccgag aggacggact
     1081 atttccgccg agttcacccc ttcccggctc cgcagactga gtttgacggt atgcgagtcg
     1141 acttcgcgga acaacaaggc tacttccacg ccctcgatat tccgcgcgaa gttgatcagg
     1201 ccctcggtat cctcttccag ggcctcggat tcggccatgt ctgcggcggt taccgtgcca
     1261 atggcaatgc ggccgccatc cagcagggta acgctgttca caaaacgatt caggaggcgg
     1321 aaccgcgccg gagacatctc atcgaagacg cgccgcccga tttccgcaac cggcaggccg
     1381 gtggcgacca ggcgggccgc gatgcgatgg gtccgttcgg tagtgtttga gtatttgaag
     1441 cttccggtat cggttgccag cgcgacgtag gcgcattcgg cgatctcgcg cgtcaggctg
     1501 actccggcac aatccatgag gtcgatgatc aattcgccgg tggcggcgta tcggggatca
     1561 accacggcca ggtcgccgat gggatcatct tcgaggtggt ggtcgagcac gatgacttcg
     1621 gtgttctcat cgatcaaatc actcacctcg ccgatgcgat ccagctgagc cacgtcgaca
     1681 attacggcca ggtcaaaagg cggagcgcag acttcgggcg ggcagatacg ctccgctccc
     1741 ggcagccagg cgtagcgctt gggaacggga tgggctagag agcagacgac ctcggggtca
     1801 tgcagggtgc ggatgatgtg gtacacggcc agcactgctc caacggcgtc gccgtccggt
     1861 gagacatggc tggtcacgag aatgcgtttc gcctggcgaa ttctctgcag aatttccgcc
     1921 agcttactct gcggcatccc tgtcgtcctg ttcttccgca tggatttcct tgaagatctc
     1981 ctccagacgg aacgcatcgt ccacggaggt atcttcaaaa aagcggattt cgggcgcatg
     2041 gcgcatgcgc agacggcggc cgacctcgcc gcgcagccag gttgcggagc gccgcagggc
     2101 ggccagggag gctttgcgct gtttggattc gcccataagg ctgacatacg cattggcata
     2161 acgcaggtcg ggcgacatgc gcacatgcat caccgataca aaaccgatgc ggggatcttt
     2221 cagtccatgc gtgagcaggt gggcgatttc ctgccgaacc agttctccga cgcgcttttc
     2281 tctgccgtgg ctcatagtct aaagcatttc gatagcgatg tttgtgactt cagcttcgct
     2341 gcatgtcgcg gcgtagcgtc cgatctcgtt aagctgggta ttcgcaaagc ggctttcgcg
     2401 ggcaaccaca cagacggcca gccgggcccg ccgccattca tccaggaagt ctacctccgc
     2461 gaccgacgca ttgtaccggt ttcggatgcg gtccttcagg ctttttacca cccggcgttt
     2521 gtctttgaga gaacgcgcgc cgtgaataag cagatccaat gtcagcaacc cgatggtcaa
     2581 tggaatgcct ttgttctatg aggcgcaggc cgtcgggcta ctccagcttc tgcgctacct
     2641 gctcgaggcg gtacgcttcg atgatgtcgc ccaccttgac gtcctggaag ctctccagca
     2701 cgatgccaca ctcgaaccct gtagcaacgc tgcgcacatc gtccttttcc cggcgcagcg
     2761 aggcaatcct gccttcgtac agaaccacat cattccggac gacacggata agtgaggcgc
     2821 ggctgatctc accgtcctgc acatagcaac cggcaatgtt cccgatggcg gacgatcgaa
     2881 agatctcgcg aatctccaca tggccggtaa cgacttcctt cgtttcggga gtcaacaggc
     2941 cttccagcgc cttcttcacc tcgtcgatca cttcataaat gatgcggtag gttcggatat
     3001 cgacgccttc ctgttccgcg agctttcgca cccgcgggtt ggcggtcaca tggaagccga
     3061 taatgacggc gtcgcttgcg ctggccagga tgacatcgga ctcattgatg cctcccacac
     3121 cgctgtgaac cagctccact ttgacgtctt cgttgccaag cttcagcagg ttgctttcca
     3181 gtacatcgac cgagccctgc acatcggcct tgatgacgac gttcaaaacg tggcgttcac
     3241 cttccttcag gtgcttatgg aagtcttcca gggtgatgcg gcgcgcggtc gctccctgct
     3301 tcagcttcgc ccggtcaatc cgtttctccg caatcgcccg ggcaatgcgt tcgtcgtcta
     3361 caacgacaaa ctggtcgccc gcggtggcca cgtcattaaa cccgagcaca agcaccgggg
     3421 tcgcaggagg cacgttctcc acttgctctc ctcgcgaatt gatcatcgcg cgtacgcggc
     3481 cgtaggtctc gcccgcgaga aacacatcgc ccacgtggag cgtgccggtt tgcaccagga
     3541 cccaggccac ggggccctgc cctttggaaa gttcgctttc aaccacggca ccgcgggctt
     3601 tctttcgagg attggccctg agctcgagca tctccgcttc caggacgagc atttccatca
     3661 gggtatccac gccctcgccc gttttcgcgg agatgttccg gatgatcgtc ttgccgcccc
     3721 attgttcgtc tacgaggtcg tatttcacca gctcctggcg gatgcggtcg ggctgggcct
     3781 ccggtttgtc gcatttgttg atggcgacca caatgggaac ctcggcggcc cgggcgtggt
     3841 caatagcctc gacggtctga ggcatgatgc cgtcgtccgc ggcgatgacc agaacaacca
     3901 cgtcggtggc ctgtgcgccg cgggcgcgca tctgcgtaaa ggcggcgtga ccgggcgtgt
     3961 ccaggaagac aacccgcccg cctcgaatct ccacgtcata ggccgcgata tgctgggtaa
     4021 tgccgccgac ctcgccctcc gcgacatggg cccgccgcag gtagtcgagc agggtcgttt
     4081 tgccgtggtc cacgtgtccc ataaccgtga cgacgggcgg tctgagcacc agatcctcgg
     4141 gcgcatcggg ctcttcggcc atgatttctt cttcgcctgg gatggcactg cggacttcgt
     4201 atccgtgggc ttccccgatc tggcggataa ggtcgaacga gaggctctgg tttttattgg
     4261 ccaggatgtt gtgctccatg agctccagaa tgacgtcgct cgtggccata tccagggctt
     4321 cggccaactg ctccaccgtc atggtctcat cgacttcgat gatgtgcttt tcaaagatct
     4381 catcaacggc actgcccagt tcgtcatctt tgtcgcgctt tttacggcgc tttttaatct
     4441 gcccggttgg tccggcggcc tggaactcgc gtaccttcgc ggccgcgtcc cggcgcatgg
     4501 cttcttccgc acgctggcgt tcgactctct tctgacgctt gcgcgcggtc tttccggtga
     4561 cggtgcgctt ggtcgcaggg gcagccgtgg cggccgtcgt gaccgttgcg gccagttctg
     4621 cccgttgttg ccgcttgcgc gccatgcgtt cttcagcgcg ccgcttcacc tcggccacca
     4681 ccgcggggtc gggcttgacc atcgcccggg ccgcctcgcg cgctttgcgc gcttcttctt
     4741 cttcctgatg gcgctccgct tcagcgagaa tggcgctctc aagttccgcc tcgtcgtctt
     4801 caaatacggg cgtttcttcc aggacggcat ccaactcttt cacacgcgtt ccgtcggcac
     4861 ggataatctg gacgacgtcc tcatggtcga cggcccgccc gatcaggatg tcgggctcgg
     4921 tcgttcgcgc gggcttttta gctgccgctt tcttgggggc agcttttttc gtttcagcct
     4981 tcccggcggc ctcggccgcc gccgccggag cctcctcctc ggcttcaggg cgcaggattt
     5041 ccaccagggg cgattcttcg ggttcgtcct cgctcttctt tttcttcgcg gcggctttcg
     5101 tggaagcggc tttcgctttg gtcggcgtct tcttggctgc gggcttcttt ttcgcggcca
     5161 gcttgcgttt ggccgccgcc ttcttcgcgg cggcttgcag gcgttccgta cgtttccggg
     5221 ccttctcttc ttcatcgagc ttcttttggc gcacccgttc ggcaacggaa gggtcgtcgt
     5281 caacgtcgat gagcaagtcg cactgatcgt cggtcaactc gctgtcaacg ctctcgacct
     5341 ccatcagcat gaagcggagt ttttcaaggg cttcctccgc tgtcatgtcc agccgtttcg
     5401 ccaagctcgc tactgtctgc attaagtcta actcctgaag aaccttactc ttcgcattgc
     5461 atcagaaacg cctattacat tacagatctc cccgtgtcgg ttcgtttgag gcagggccat
     5521 cggcatccgc ctcctccttc aacggttcgt cttcatccac atctgccgtc tcatctgtat
     5581 tcagaggggg ggcgtcttcc ggcggggcgc ccgtcgagcc ggcgtccggg gcggcgccac
     5641 ccgtcagttc aacatcgctc ttgatatcga tgttccagcc cataagtttt gaggccaggc
     5701 gcgcgttctg cccccgtttt ccgatcgcca gcgacaactg gtcatgaggc acgaccacca
     5761 gaattacccg gttggtttca tccacggaaa tgtccaggat atccgccgga ttcagtgcgt
     5821 tggtgctcat ttccaccgga tcttcgctcc agcggacaat atcgatcttc tcgccgcaca
     5881 gctcctcaac aaccgcgcga acccgcgacc ctttcatgcc tacgcacgcg ccgaccgggt
     5941 ctacgttaag atcgttggag tggacggcga tcttggtgcg gttgccggct tcccgggcga
     6001 tccctttgat ctcgacggta ccatcgtaaa tttcggggac ttccagctca aaaagcgcgc
     6061 gcaccaggtc gggacaggcg cgcgacaaga ccacctgagg gccgcgggga gacttctcga
     6121 cttcgacaag aaacgcgcgg atgcggtctc cgggtttgaa gttctcccgg ggggactgtt
     6181 cccggagcgg cagaacggcg tcggcccggc caatggagac gataatattg ccgtggctga
     6241 tgcgtttcac cacgccggtg accatctcgc cttcccgctg cttgaactcg tcgtacacat
     6301 tttcacgctc agcgtctttg agcttctgaa tgatgacctg tttggcggtc tgggctgcga
     6361 tacgcccgaa atccttgggc tccgccggta ctttgaggcg gtcaccgaca cccgcctcgg
     6421 gattcatgcc ctgggcctct tcgacggtca tttcccgggt gtcgtcggtc acgctttcca
     6481 cgacttctcg aatctcgtat accttgaaga cgagcgaggc gggatccact tcaacgacga
     6541 tttcggcctt gggatggaag ctctttcgcg cggcactttc gagtgccgtg cgaatggctt
     6601 ccagcagctt gtcgcggtcg atgctctttt ccgcttcgag ctgctgt
//
LOCUS       DAOPBQ010000157         6614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10294_length_6614_cov_3.505565, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000157 DAOPBQ010000000
VERSION     DAOPBQ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10294_length_6614_cov_3.505565"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            423..1310
                     /locus_tag="PKW60_06565"
     CDS             423..1310
                     /locus_tag="PKW60_06565"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPJ99131.1"
                     /translation="MKKTAVLMAVALLASAPGWAAFITDLGQNITIPDGVTGSGAWYT
                     GVGQAGEDNEVEPGCTTGQLWDLEAFYLNQNNFLTMVGGYDFENGASGSGHTWKYGDL
                     FIDVNNNAVFGQAAAAISTGSQGNNPDSIANTFGYDYVLTFRGDTGVDGNPLYRAYKL
                     DPNDSTLLVYYNQNAAANPWRYDSGGTELFNNGSFSYFTGLGDDLDGDGVADVLGGTH
                     NAVRVDLTWLWDEVRKDNPQWNGLITTHFTMECGNDNLMGQGVLPYDPGTPVPEPASL
                     TLMGLGIAAVAVSRLRKKA"
     gene            complement(1401..3671)
                     /locus_tag="PKW60_06570"
     CDS             complement(1401..3671)
                     /locus_tag="PKW60_06570"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPJ99132.1"
                     /translation="MLFDFDMIQEVYAGLPGRVNAARELLARPLTLSEKVLYSHLFGS
                     PPAEPFVRGASYVDFRPDRVAMQDATAQMALLQFMQAGKKRTAAPTTVHCDHLIQARS
                     GAGPDLAAALSVNKEVFDFLASVSNKYGIGFWKPGAGIIHQVVLENYAFPGGLMIGTD
                     SHTPNAGGLGMAAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGRLNGWTAPKDVILK
                     VAGILTVKGGTGAIVEYFGPGSESMSCTGKGSICNMGAEIGATTSLFAFDDSMVRYLE
                     ATGRGAVAEAARAIRDYLRPDPEVYVNPEQFFDQVLEIDLSTLQPHLNGPFTPDLATP
                     VPAMREAVKKNGWPEKVEVGLIGSCTNSSYEDMSRAASIARQARAKKLRAKSEFTITP
                     GSETVRYTMERDGLLGEFEAIGGVVLANACGPCIGQWARHRPGPEDEANTIVTSFNRN
                     FAKRNDGNPKTHAFVASPDMVTALVLAGDLGFNPMTDTLINEDGEEVRLEPPCGEELP
                     PKGFEVDDPGYQEPAADGSALEVVIRPDSERLQRLEPFAPWEGADLKGLRLLLKARGK
                     CTTDHISMAGPWLRYRGHLDNISNNCFIGAINAFNGEANRVKNQLTGVYDEVPRVQRA
                     YKAEGIGTVVVGDENYGEGSSREHAAMEPRHLGVRAILVRSFARIHETNLKKQGMLAL
                     TFANPADYDAIQEDDVIDIVGLTGFAPGVPLTVVLHHTDGTTYEFSVNHSYNKAQIAW
                     FKAGSALNLIKAQAGR"
     gene            4371..5105
                     /locus_tag="PKW60_06575"
     CDS             4371..5105
                     /locus_tag="PKW60_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPJ99133.1"
                     /translation="MPTLVVWTSIMLGLCLLCRCLAKLRSREAELRVRSGFVRTWARI
                     SMRLAGFRIHLVGPPPPRHAFTVSNHLSSIDSVLIAAVTGGVFVARHDMAQWPGIGFL
                     TRHMDIIFVNRSRRTEVGGINEQIASALHDGFAVHVFAESRIGDGKTVQAFKPALLEA
                     AVRADIPVHYAILDYRTRPGDPPPSQAVHWGPGVSFIEHILGILRLKGGEAFVTFGDE
                     PVAGTDRKVLANALWEKVNSRFIPLE"
     gene            5382..6392
                     /locus_tag="PKW60_06580"
     CDS             5382..6392
                     /locus_tag="PKW60_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPJ99134.1"
                     /translation="MRHKQGFTLIELLVVIAIIGILAAILLPALARAREAARRASCAN
                     NLKQMGLVFKMYANESPGEKWPMVQIGLFRNDFSGEEGAALDFCPMIPAIYPEYLTVP
                     WVLVCPSSPKMDFEDRIYVNDTGNTGKAPDAVFCFGDYDTHGGSCMRAADESYTYYGW
                     LFDAWDVPLVPLAPLATVVNPLLKPNEQLKPTDQGPGQFVFWAAALISGCKPYLDAND
                     PVGLARHADSDINLAALPGGIGAGYGTSGGNVIYRLREGIERFLVTDINDPASTARAQ
                     SDVFVMWDMLSTNPKDFNHVPGGSNVLYMDGHVEFVRFPGKQPVNSAVALLAGALTGA
                     GN"
BASE COUNT         1288 a         2067 c         1944 g         1315 t
ORIGIN      
        1 cctggccggg gagttcctcc ccggcctttt tgtgtctgcc cgccccgccg gctgccctcc
       61 atcggcctgc gccccgctcg aattgagggc gtaacccgcg tggaaccctg cgcttgactc
      121 ccgcccctcg gccagactga aaaactgccg tgtcacgctt gaccactctt tatcggacag
      181 caaattgttt gtcacggatg accaactcca gtcgcaatac atcggtcttt gcctgaccac
      241 tgcctcagcg cgaacagcat aacgccgtgc tattcttcat attacaccga cccgcccaag
      301 ttggcgcgaa tcttgctttt tcgtgcgcga gggtgtttgc ggcaaatctc gtgggccctg
      361 gcataaacgg caatcggatt ggaaaaacga ggcaactcac aaagcgaatg ggagaatacg
      421 cagtgaaaaa gacagcggtt ttaatggcag tggcgctcct tgcgagcgcg cccggatggg
      481 cggcattcat taccgacctc ggccagaaca tcaccatccc cgacggtgtt acgggttctg
      541 gggcctggta caccggagtg ggacaagccg gtgaggataa tgaggtcgag cccggctgca
      601 caacgggcca gctatgggat ctggaagcct tctatctgaa ccagaacaat ttcctcacca
      661 tggtcggcgg ctacgatttc gaaaacggcg cttccggtag cggtcatacg tggaagtacg
      721 gcgacctgtt tatcgacgtc aataacaacg ctgtcttcgg acaggctgcc gccgctatca
      781 gcaccgggag tcaaggaaac aacccggact ccatcgctaa cacgttcgga tacgactacg
      841 tcctgacctt ccgcggcgac acgggtgtgg atgggaaccc actgtaccgc gcttataagc
      901 tggacccgaa tgactcgacc ctgcttgtgt actacaacca gaacgctgca gcgaatccgt
      961 ggcgttatga ttccggcggc acagaactgt tcaacaacgg aagtttcagc tacttcacgg
     1021 gcctggggga cgacctggac ggcgatggcg tggctgatgt gcttggcggt acgcataacg
     1081 cggtacgagt cgacctgacg tggctgtggg acgaagtgag gaaagacaac ccccaatgga
     1141 atgggctgat caccacccat ttcacgatgg aatgcggcaa cgacaacctg atgggccagg
     1201 gcgtcctgcc gtatgacccc ggtacgccgg ttcccgagcc ggcatccctc accttgatgg
     1261 ggctggggat tgcggccgtt gcggtctcgc gcctgcgcaa aaaggcataa acgaaccgaa
     1321 cctgaattct gcagtaacaa cttggccggg aaggatctcc tccccggcca tgtctttttt
     1381 tcccgcccgg cgccgacttt ttatctgccg gcctgcgctt tgatcaggtt gagcgccgag
     1441 cccgccttga accaggcgat ctgggctttg ttgtagctgt ggttgaccga aaactcgtac
     1501 gtcgtcccgt cggtgtgatg cagcaccacg gtcaagggga ccccgggcgc aaacccagtc
     1561 agccccacga tatcgatgac gtcgtcttcc tgaatcgcgt cgtaatcggc gggattggca
     1621 aaggtcaggg cgagcatgcc ctgctttttc aggttggtct cgtggatgcg cgcaaacgac
     1681 cggaccagga tggcccgaac gccgaggtgc cggggttcca tggccgcgtg ctcacgagac
     1741 gacccctcgc cgtagttctc gtcgcccaca acgacggtgc cgataccctc cgccttgtag
     1801 gcgcgctgaa cccgaggcac ttcatcgtag acgccggtca actggttttt gacccggttg
     1861 gcctcaccgt tgaacgcgtt gatcgcgcca atgaagcaat tgttggagat gttgtcgaga
     1921 tgtccgcggt agcgcagcca cgggccggcc atcgagatgt ggtcggtcgt gcacttgccc
     1981 ctggccttga gcagcagccg caaccccttc agatccgccc cctcccaggg ggcaaagggt
     2041 tcgagccgct gcaggcgctc cgaatcgggc cggatcacca cctcgagcgc gctgccgtcc
     2101 gcggcgggtt cctggtaacc cggatcgtca acctcgaacc ccttcggcgg cagctcctct
     2161 ccgcaaggcg gttcgagccg gacctcctcc ccgtcctcat tgatgagggt atcggtcatg
     2221 gggttgaatc cgaggtcgcc ggccagcacc agggccgtca ccatgtcggg cgacgccaca
     2281 aacgcatggg tcttcgggtt gccgtcgttg cgtttggcaa aattgcggtt gaacgaggtc
     2341 acgatggtgt tcgcttcgtc ctcgggaccc ggccggtgcc gcgcccactg cccgatgcag
     2401 ggcccgcacg cgttggccaa caccacccct ccgatggcct cgaattcgcc cagcaacccg
     2461 tcccgttcca tggtgtagcg cacggtttcc gagccgggcg tgatggtaaa ctccgatttg
     2521 gcccgcagtt tcttcgctcg ggcctgccgc gcgatggatg cggcccggct catgtcttcg
     2581 tatgaagaat tcgtgcatga accaatcagc ccgacctcca ccttttccgg ccagccgttc
     2641 tttttcacgg cctcccgcat cgcgggaacc ggcgtggcca aatccggcgt gaacgggccg
     2701 ttcagatgcg gttgcagggt cgacaggtcg atctcgagca cctggtcgaa aaactgctcc
     2761 ggattcacat agacctcggg atccgggcgc aggtagtcgc ggatggcccg ggccgcctcg
     2821 gcgaccgcgc cgcgtccggt tgcctccagg taccggacca tggagtcatc aaacgcaaac
     2881 agcgacgtcg tggcgccgat ctccgcgccc atgttgcaga tcgaaccctt gccggtgcac
     2941 gacatgctct cggaacccgg tccgaaatac tccacaatgg cccccgtgcc gcctttaacc
     3001 gtcaggatgc ccgccacctt gaggatgacg tccttgggag cggtccatcc attcaggcgc
     3061 ccggtgagtt tcacaccaat gagcttcggg aacttcagct cccatgccat gccggccatc
     3121 acatcgaccg cgtccgcccc gccgacgcca atggcagcca tgccgaggcc gcctgcgttg
     3181 ggcgtgtgcg aatcggtacc aatcatcagg cctccgggga acgcataatt ctccagcacg
     3241 acctggtgga tgatgcccgc gccgggcttc cagaagccga tgccgtattt gttcgatacc
     3301 gacgccagaa aatcaaaaac ctctttgttc acgctgaggg cggctgcgag atcggggccc
     3361 gcgcccgatc gagcctggat cagatggtcg cagtgcaccg tggtgggcgc cgcggtccgc
     3421 ttcttgcccg cctgcatgaa ctgcagcagg gccatctggg cggtcgcgtc ctgcattgcc
     3481 acccggtcgg ggcggaaatc cacgtaggag gcgccccgta cgaaaggctc cgccggagga
     3541 ctgccgaaaa gatggctgta gagcaccttt tcactgagcg tgaggggccg cgcgagaagc
     3601 tcccgcgccg cgttcacccg ccccgggagc cctgcgtaca cttcctgaat catgtcgaaa
     3661 tcaaacagca tgagacttcc tttccgtgcc actccaggct gcccctgagc cggacggcgc
     3721 cacccactcc agcagacact ccccgcgctt aaaccgaacg gcggcagctc aaatcataga
     3781 tggccttcaa tcatacaacg cccgcgcgga ccctgcaatt tccagcgccg ccggcacaat
     3841 tcacgttcta agcgcacggg agacgattct tcgtttctgc aggaagatgt cattgagtgt
     3901 tcggcatccg aggcattttt tgggccggtt gtttagttca tcggctatat cctgtagttt
     3961 ttctaatgta ggggcagacc cccggcgcgc atttcgcggt gttcccgccg aaagttttcc
     4021 ctcccgttgt aggggcagac ccctgtgtct gcccatccgg atcgccccca tggctgcgtt
     4081 aaagaagggc agacacgggg gtctgcccct acgcgctacg gaaaacagtc cgcacatgac
     4141 ggctctgacg aaaggcaggt ccagggacct gcctccggca ccctgcccgg agcactccca
     4201 gcgcggcgat acccgcggac ctgagcgagc ccccagctgg cgagaagaat gagtagtggg
     4261 cgcgggttgc tgccggaaaa gccgtatgcc atgatgctgg ccgaggattc cggttgaggg
     4321 caaggtgcaa tatgaacagc ttgtggggca gcattcgcgc cggatggcgc gtgccaacgc
     4381 ttgtggtgtg gacaagcatc atgctcggac tttgcctgct ttgccgctgt ttggcgaagt
     4441 tgcggtcccg tgaggctgag ctgcgtgtgc gcagcggctt cgtgcgcacg tgggcccgca
     4501 tatcgatgcg cctggccggg ttccgcatcc acctcgtcgg ccctccaccg ccgcggcacg
     4561 ctttcaccgt cagcaatcat ctgagctcga tcgacagcgt gcttatcgcg gcagtgaccg
     4621 gcggcgtctt tgtggcccgg cacgacatgg cccagtggcc gggcatcggc tttctgaccc
     4681 gccatatgga catcatcttc gtcaaccggt cgcgccgcac ggaggtcggc ggcatcaacg
     4741 agcagattgc cagcgccctg cacgatggat tcgcggtgca tgtcttcgcg gaaagccgga
     4801 tcggggacgg aaagacggtc caggcgttca aaccagccct gctcgaagcc gcggtacgtg
     4861 cggacatacc ggtgcattac gccatactcg attaccgcac ccgtcccgga gaccctcccc
     4921 cctctcaagc cgtacactgg ggacccggcg tctcattcat agaacacatc ctgggcattt
     4981 tacggctgaa gggcggcgag gctttcgtga ccttcggaga cgagcccgtt gcaggtacag
     5041 accggaaagt gcttgccaac gccctctggg aaaaggtgaa cagccggttc atccctctgg
     5101 agtaaagcgc cgccggcatc ggtctcgcgc ggcacatcag gtctgccgtt caggcctgcc
     5161 actgactttc atcctctgca aagccctgta gaaagcacgc acgcgccgtg cacctcgcca
     5221 tttcgaagaa ttcagctcag ctgtctcaga aagagtgcct atatagtaca gataaagata
     5281 ttgacatgat agagaatatc tagcatactg gtaattaagg atgattcctt tgagtaagaa
     5341 ggaatcgaac tgcagtccag tggaaaacgg aggatatatt catgagacac aaacaaggat
     5401 tcacgctgat cgaactgttg gtggtcattg caatcattgg cattcttgcg gccatcctgc
     5461 tccctgcact ggcgcgcgcc cgggaagcgg cccgccgggc aagctgcgcc aacaatctca
     5521 aacaaatggg actcgtgttc aagatgtacg ccaacgaaag cccgggtgaa aaatggccta
     5581 tggtgcagat cgggcttttc aggaacgatt tcagcggcga ggagggcgcc gctctcgatt
     5641 tctgcccgat gattccggcg atttatcccg aatacctgac ggttccctgg gtgctggtgt
     5701 gcccctcaag ccccaaaatg gatttcgagg atcgaatcta tgtgaatgac accggcaaca
     5761 cgggtaaggc ccctgacgcg gtcttctgct tcggcgatta tgacacgcat ggcggctcct
     5821 gcatgcgcgc ggccgacgaa agctatacct actacggctg gctcttcgac gcatgggatg
     5881 tgcccctggt gcccctggcg cctctggcta ccgtggtaaa ccctcttctg aaaccaaatg
     5941 agcagcttaa gccaacggat cagggaccgg gccaatttgt cttttgggct gccgctctca
     6001 ttagcggctg caagccttac ttggacgcga acgatcccgt aggcctggcc cggcacgcgg
     6061 acagcgacat aaaccttgcc gctcttcctg gcgggatagg ggcaggttat ggaaccagcg
     6121 gaggtaatgt catttaccgt cttcgcgagg gcattgagcg atttcttgtc accgacatca
     6181 acgatccggc aagcactgcg cgcgcccaaa gcgacgtgtt cgtgatgtgg gatatgctct
     6241 ccacgaaccc gaaggacttc aatcatgtcc ctggcggcag caatgtgctc tatatggacg
     6301 gccatgtcga gttcgtgcgg tttccgggga aacaaccggt gaacagcgcc gtagccttgc
     6361 tggcgggcgc gcttaccggg gcgggcaact gaaaacatag cgtatgcgag cgccccggtt
     6421 gtaaacacag ccggggcgct ctcgtcctcc taatgcaacg ctgcgatccg gagccgcgtt
     6481 cggcagcagg ctggcttctc aggcgctatt ccagaattga tgcactgcgt cccgctttca
     6541 tgtgctgtga acgaattcgc gggcacatgc cacaaaaccg gccgcaccga aattttcctc
     6601 ccttgattca cttc
//
LOCUS       DAOPBQ010000158         6611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10298_length_6611_cov_4.295607, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000158 DAOPBQ010000000
VERSION     DAOPBQ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10298_length_6611_cov_4.295607"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2431
                     /locus_tag="PKW60_06585"
     CDS             <1..2431
                     /locus_tag="PKW60_06585"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPJ99135.1"
                     /translation="EIQDCLGRITARGESPVTADGHAAIDINFLRPVVVPHRLTARLR
                     GETELLATAHLPFTATVPYPYDDFTVLMWSYAEGDLTLRNENRLCYELGSDMMDLCHM
                     RGYNDAGAAREYAVAAESGQRIVPYVTRIAGEATEDHLLKPSLFDQGWIQRERASMEI
                     SCRQAAPYQPPAYTLGDENYLARSGTEVDLSPENIAAFKAWLQQRYPNIEALNAAWKT
                     SHASFDNLGEPMLLEEAQDETASFAPWFDFHCFMDDAFADLHETLADFVRAEDPGAKV
                     GWDGFLAYHWQAGYDFYKLSRNLELNQVYTTYPIQGELVRSFKQPGALTGEWGNAVAD
                     KEDGFSAILWHTLFRGHNSGWWWTSWGCDYIPFNPDMSVSHMGKWFFDSAAEIKAGPG
                     RLLLHAQRDDSGIAVLYNQADLFAARLLSAMQHDTPLPDWQRNLIGVMHALKDAGFQY
                     TFVAAAEIEAAPARLDGYRALILPLATCLSESAVQAIHTFAHRGGVVIADGRTAQLTG
                     NGEIREQRPLDELFGVQSPAGLDAFKAPPETGAFELVGEPLEASVLEPGITPANGRAE
                     ATGKAPCFITRAVGDGHAVLLNVPFTTATALRTEGRERTLLDPLTRYLALADIKPFAQ
                     LTVEGQPARCIEQILFEDGALRYLALQQDILVRNLEPQELTVTIDAPAFVYDVRAGRA
                     VADTPITSWNTTISRGNPRLFALMPYAVAGLEVDVAPECALGGTLEMAAAVSVEGVQP
                     QFHVVHVDVFAPGSDHPHREYSQNLPCALGRGKGTIPFAQNDPAGAWRLVFRDTATGT
                     STEATVYLSDR"
     gene            2783..4582
                     /locus_tag="PKW60_06590"
     CDS             2783..4582
                     /locus_tag="PKW60_06590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPJ99136.1"
                     /translation="MPSKSHFLRKAALVAGLSFICLSVAVQAEPAPEVATVMDGIVER
                     MYQSLNIDELAALDNESIWSFITPEERTVLATKYWTFDVNVPVVVSVMRHNEQAVMPF
                     WLTEAGFQKTALTVRNDHYTYEVWQKRFDAGRVELGINGFDKHRPHYFVAVGPQRTGT
                     PLRVTNLMPAEYSKSLMGVGALTYHDWTELVLEEVPPELTGHVLLTTVRGRAREAHLI
                     GAFRQTAHPSSEQPDLVALTWSDDPTTTQTIQWRNSTAVERGAVQYRPQQASDSTPWQ
                     TASAERTVIKDRLLANDPVCHRFTATLTGLQPGTAYTYRAGDPARGTWSGEAVFTTAP
                     ASPDPFTFVFIGDSHCSPHYGEMLGQAFTRHPETAFYLLVGDLINTGLYRDDWDKFFA
                     LSTEVFRQRPVMPCIGNHDDQDGLGAGMYLNMFGLPKNGPDGVQPERMYSFEYGNALF
                     LILDIATPLETQAAWMESRLANSSATWKFAMVHFPPYAPTEDYPRMRAIWGPVFDAHH
                     VDVVFAGHVHHYLRSKPLYAGKPVASPAEGTIYLVSVGIPSRDWHGEEAPYADVIFCG
                     PPLYQTLRIDGNTLELHTYSADGQEQDQLTITK"
     gene            complement(4597..5559)
                     /locus_tag="PKW60_06595"
     CDS             complement(4597..5559)
                     /locus_tag="PKW60_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPJ99137.1"
                     /translation="MGIARKTGVIAVIIALAAGTFQCGQGGPRPSGEGAAGADGKLVI
                     GVSLLNLSNEFIVLLEEAMKEEAAKQGVRLIVNDGNRSAERQVQQIENFLLQKVDAII
                     LNPCEHEASSPAVDKALAAGVPIVNVNSQTRSTPTAFVGSRDEESAQIAMQYIAERLG
                     GSGNIVMMQGFMGQTAQIDRERGAQEVLTQYPQLNLLAAQTAEWDRAKAVTLMENWLQ
                     SYGDQINAVFAQNDEMGMGALLALEQAGVKERVVVASVDAIADALEAIQAGRLDATVF
                     QDARSQGSMAVQTACAIVSGQPFDNEVFIPFRLVTQDNVAAYLR"
     gene            complement(5597..6571)
                     /locus_tag="PKW60_06600"
     CDS             complement(5597..6571)
                     /locus_tag="PKW60_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ99138.1"
                     /translation="MSLDDSKTQDELLRQRPGQGAWRRFGLVLALVAVCVVLAAARPR
                     AMSIDYVLTLLRQVAINGILAVGVTHVLLTGGVDLSLGSVVALAGVVAATFAHPDQYP
                     LALPLLLGLCTGAVCGVVNGLVVTWGRVAPFIATLGMMTAARGLAYIFSGGRPVSRLR
                     PEFNAIGSGSLLGIPIPVVLLAVVALLSMVFLRYTRAGRHLYATGGNEVAAYASGINT
                     RRVKVLAYILCGTLAGLAGIVQAARSTTGQPNAGMMYELDAIASVVIGGTSLAGGVGG
                     VGGTLLGALLMGVISSGLDMLNVPSYYQQVAKGLIIIGAVMLDRRGSK"
BASE COUNT         1338 a         2124 c         1898 g         1251 t
ORIGIN      
        1 cgagatccag gactgtctgg gcaggatcac cgcgcgtgga gaaagcccag tgacggccga
       61 tggacatgcc gcgattgaca tcaacttcct tcgcccggtc gtcgtccctc atcgccttac
      121 cgcgcgtctc cggggcgaaa cagagctgct ggcgacggct catctgccgt tcacggccac
      181 agtgccctac ccgtacgacg attttacggt gctgatgtgg tcctatgcgg aaggggatct
      241 caccctgcgc aacgaaaacc gcctctgcta tgaactcggc tcggacatga tggatttgtg
      301 ccacatgcgc ggctacaacg atgcgggcgc cgcccgcgaa tacgccgtgg cggccgaatc
      361 gggccagcgt atcgttccct atgtgacgcg cattgcgggg gaagccaccg aggatcatct
      421 cctgaaaccc agtctgttcg atcaagggtg gattcagcgt gagcgggctt caatggaaat
      481 cagctgccgc caggcggcgc cgtaccagcc gccagcgtac accctgggcg acgagaatta
      541 cctggcccgc agcggtaccg aggtcgactt gtcgccggag aacatcgcgg cgttcaaggc
      601 gtggctgcaa caacgctatc ccaacatcga ggccctgaac gcggcctgga agaccagcca
      661 cgccagcttc gataacctcg gcgagccgat gctgctcgaa gaagcccagg acgagaccgc
      721 ctcttttgca ccgtggttcg acttccactg tttcatggat gacgcgtttg cggacctgca
      781 cgaaaccctg gcggattttg tgcgcgcgga agatccgggg gcaaaagtcg gctgggacgg
      841 attcctggcc taccactggc aggccggata cgatttctac aaactttcgc gcaacctgga
      901 actcaaccag gtgtacacga cctacccgat ccagggcgaa ctcgtgcgtt cgttcaaaca
      961 acccggcgca ctgaccggcg aatggggcaa tgcggtcgcg gacaaggaag acggtttctc
     1021 cgcgatcctc tggcacactc tgtttcgcgg gcataattcc ggctggtggt ggacctcctg
     1081 gggctgcgat tacatcccct tcaacccgga catgtccgtc tcacatatgg ggaaatggtt
     1141 tttcgacagt gcggcggaga tcaaggcagg gccgggccgt ttgctgctcc acgcccagcg
     1201 ggatgattcg gggatcgcgg tcctctacaa ccaggccgat ctcttcgcag cgagactcct
     1261 gagcgccatg cagcacgaca cgccgcttcc cgattggcag aggaatctga ttggcgtcat
     1321 gcacgcgctc aaagacgctg gattccagta taccttcgtc gctgccgcgg agatcgaggc
     1381 ggctcccgcc cgcctggatg ggtaccgggc gctgatcctg ccgcttgcga cgtgcctgtc
     1441 cgagagcgca gtacaagcca tccatacgtt tgcacatcgg ggcggcgtgg ttatcgcaga
     1501 cggccgcact gcgcagctta cgggcaacgg cgaaatccga gagcagcggc cgctggacga
     1561 gctgtttgga gtgcagtctc cggcaggcct ggacgccttc aaagcgcccc cagagacagg
     1621 cgcctttgaa ctggtgggcg agccgctgga agcaagcgtg ctggagccag gcatcacgcc
     1681 ggcgaatggc cgggcagagg caaccggaaa agcgccctgc ttcatcacgc gtgccgttgg
     1741 cgacgggcat gccgtgctgc tcaatgtccc cttcacgacc gccacggccc tgcggaccga
     1801 ggggcgcgaa cgcacgctgc tggacccgtt aacgcgttat ctggcgctgg cagatatcaa
     1861 gccttttgcg cagctgactg tagaaggcca gccggcccgt tgcatcgaac aaatcctctt
     1921 cgaggacggc gcgctgcgtt acctggccct gcagcaggac atcctggttc gcaacctcga
     1981 gccacaggaa ctgacggtga ccattgacgc accggcattt gtgtacgatg tgcgtgcggg
     2041 acgcgccgta gcggacacac caatcacgag ctggaacaca acgatctcac ggggaaatcc
     2101 gcgtctattt gcgctcatgc cgtatgcggt ggccggtctc gaagttgacg ttgcgccgga
     2161 atgcgctctt ggaggcacgc tcgaaatggc cgcggccgtt tccgtagaag gcgtacagcc
     2221 tcaatttcac gtggtgcacg tcgatgtctt cgcgcccgga agcgaccacc cgcaccgaga
     2281 atacagccag aacctcccct gtgcgctcgg ccgcgggaaa ggcacgatcc cgtttgcgca
     2341 aaatgatcct gcgggagcct ggcgcctggt tttccgcgac accgccacgg gaacatccac
     2401 ggaggcgacg gtgtatctgt cagaccggta gtaatacgcg ttttatacgc tcaaaaagca
     2461 gcgccttttg aattatgtgc gcaaagcgcc tcgatcacac cataaaattc agtatactga
     2521 ggagcgttcc gttactggtg tgcaatcaag gagaacagtg atgccttcga cattagattt
     2581 tctgcgaaga cccgttttca tcgctggcct tcttttcatc gcactgacat tagtgtatct
     2641 gtcagaccgg tcgtaatacg cgttttatgc gctcaagaag cagcgccttt tcaattcgca
     2701 gcataaagcg cgtcgatctc gtcataaaat tcagtatact gagaagcgtt ccgttactgg
     2761 cgtgcaatca aggagaacag tgatgccttc gaaatcacat tttctgcgaa aggctgccct
     2821 cgttgccggc ctttctttca tatgcctgtc agttgctgtg caggccgagc cggcccccga
     2881 agtggccacg gtcatggacg ggattgtcga gcgcatgtat caatcgctaa atatcgatga
     2941 actggccgcg cttgacaacg aatccatctg gtccttcatc accccggaag agcgaacggt
     3001 acttgccacg aaatactgga ccttcgatgt gaatgtccct gtggtggtgt cggtgatgcg
     3061 ccacaatgaa caggcagtga tgccgttctg gctgactgag gcagggttcc aaaaaactgc
     3121 gcttaccgtc cgaaacgatc actatacata cgaggtctgg cagaagcgtt tcgatgcggg
     3181 ccgcgtcgag ctgggcatca atggattcga caagcaccgc ccgcactatt tcgtcgctgt
     3241 gggaccgcaa cgcacgggca cgccgctccg cgtgaccaac cttatgcccg ccgaatattc
     3301 gaaaagcctc atgggcgtag gcgcgttgac ctatcacgac tggaccgagc tcgtcctcga
     3361 agaggtcccc cccgaactca cgggacatgt ccttctcact acggtgcggg ggcgcgcccg
     3421 tgaagcgcac cttatcggcg catttcgtca gaccgcgcat ccctccagcg aacagcctga
     3481 cctcgtagcg ctgacctgga gtgacgatcc caccaccaca cagacgatcc agtggcgaaa
     3541 cagcacggcc gtcgaacgcg gcgcagtcca gtatcgcccg caacaggctt cggacagcac
     3601 gccctggcaa acggccagcg ctgaacgaac ggttatcaag gaccgcttac tcgccaatga
     3661 ccccgtgtgc caccggttca ccgccacctt gaccggactt cagccgggaa ccgcttacac
     3721 ctaccgggcc ggggaccctg cgaggggcac ctggtctggc gaagcggtgt ttaccaccgc
     3781 acccgcatct ccggacccat tcacgtttgt tttcatcggc gactcacact gttcaccaca
     3841 ctatggtgaa atgcttggtc aggctttcac tcgccatccc gagaccgcgt tctatctgct
     3901 cgttggcgac ctgatcaaca ctggtctcta ccgggatgac tgggacaagt ttttcgcgct
     3961 gtctactgaa gtgtttcggc agcgtcctgt catgccgtgc atcggcaacc acgacgatca
     4021 ggacggttta ggggcgggga tgtatctcaa catgttcggc ctgcccaaaa atgggcccga
     4081 cggggtccag ccggaacgca tgtacagttt tgaatacggc aacgccctgt tcctcatact
     4141 ggacatcgcc accccgctgg aaacccaggc agcctggatg gaatcacgtc tggcgaacag
     4201 ctccgccacg tggaaattcg ccatggtgca cttcccgccg tacgcgccca ccgaagacta
     4261 cccgcgcatg cgcgccatct ggggacccgt attcgatgcc caccacgtcg acgtggtctt
     4321 cgctggacac gtacaccatt acctgcgcag caaaccgctg tacgctggca agccggtcgc
     4381 gtctccagcg gagggcacca tctacctggt ttccgtgggc attccctcac gcgactggca
     4441 cggcgaagaa gccccttacg ccgatgtcat tttctgcggg cctccgcttt accagaccct
     4501 gcggatcgac gggaatacgc tcgaactgca cacatacagc gcggatggcc aagaacagga
     4561 ccagctgacc atcacgaaat aaggcccgcc ggacggttag cgcagatagg cggcgacgtt
     4621 gtcctgagtg accaggcgaa atggaatgaa gacctcgttg tcgaagggtt gacccgacac
     4681 aatggcgcag gcggtctgca ccgccatgct tccctggcta cgcgcgtctt gaaacaccgt
     4741 tgcatcgaga cggccggcct ggatcgcctc cagcgcgtcg gcgatcgcgt cgacactcgc
     4801 gaccacgacg cgctctttca cgccggcctg ctcgagcgcc agaagagcgc ccatgcccat
     4861 ctcatcgttc tgtgcaaaga ccgcgttaat ctgatccccg taagattgaa gccagttctc
     4921 catcaaggtg acggctttcg cgcggtccca ctcagcggtc tgagccgcca gcaggttgag
     4981 ctgtggatac tgagtgagta cctcttgcgc gccgcgttcg cggtcaatct gagctgtttg
     5041 acccataaat ccctgcatca tgacgatatt gcctgagccc cccaggcgct ccgcgatgta
     5101 ttgcatggcg atctgggcgg attcttcgtc gcgcgaacct acaaacgccg taggtgtgga
     5161 acgggtctgg gaattcacat tgacgatagg aacgccggcc gcgagagcct tgtcgacggc
     5221 gggcgagctg gcctcgtgtt cacacgggtt gaggatgatg gcatccacct tctggagcag
     5281 gaaattttcg atctgctgca cctgacgctc agcgctgcgg ttgccgtcgt ttacgatcag
     5341 gcgcacgccc tgcttcgccg cttcctcctt catcgcctct tcaagcaata cgatgaactc
     5401 gttcgacaga ttcagaagcg agacgccgat gacaagcttg ccgtccgcac cagcggcccc
     5461 ttcgccgctc ggcctggggc cgccctggcc gcattgaaat gtcccggccg ctaacgcgat
     5521 gatgactgca ataaccccgg tttttctcgc aattcccatc gtcactcttc ccctgtagtg
     5581 cccgctgcgg gcggtatcat tttgatccgc gccgatccag cataaccgcg ccaataatga
     5641 taagtccctt cgccacctgt tggtaatacg aagggacgtt cagcatatcc agcccactgc
     5701 tgatcacccc catcagcagc gcccccagaa gggttccgcc aacaccgccc accccgcctg
     5761 cgaggctggt tccgccgatg accactgatg caatagcgtc gagctcatac atcatcccgg
     5821 cattgggctg gcccgtggtg ctgcgggcgg cctgcacgat gccggccagg ccagccaggg
     5881 tgccgcacag gatgtacgca agcactttga cccggcgcgt attgatgcct gacgcatagg
     5941 ccgcgacctc gtttccgccc gtcgcgtaca gatgccgtcc cgcgcgggta taccgcagga
     6001 agaccatcga cagcaacgcc acaaccgcaa gaaggacaac aggaatcggg atccccagca
     6061 ggctgccgct gccgatggca ttgaattccg gcctcagacg cgacaccggg cggccgccac
     6121 tgaaaatgta ggcaagtccg cgcgccgccg tcatcatacc cagggtggca atgaacgggg
     6181 caactcgacc ccaggtcacg accaggccat tcacgacacc gcagaccgct ccggtgcaca
     6241 gacccagcag caggggcagg gccagcgggt attggtccgg gtgcgcaaac gtggccgcga
     6301 ccacacccgc cagcgccacc accgagccca gcgataagtc gacgcctccc gtcagcagca
     6361 catgggtgac tcccaccgcc aggatcccgt tgatcgccac ctgccgaagc agagtcaaga
     6421 cgtaatcgat ggacattgcc cgcggccggg ccgcggcgag aaccacgcag acggccacca
     6481 gcgcgagcac caggccaaag cgccgccacg cgccttggcc cggtcgttgt ctgagaagct
     6541 catcctgagt cttgctgtca tccaaactca tcgtgcattc gtgtccacgc gccgttttac
     6601 cccactcccc g
//
LOCUS       DAOPBQ010000159         6610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10299_length_6610_cov_4.466056, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000159 DAOPBQ010000000
VERSION     DAOPBQ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10299_length_6610_cov_4.466056"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1525
                     /locus_tag="PKW60_06605"
     CDS             242..1525
                     /locus_tag="PKW60_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99139.1"
                     /translation="MSRAKKSQQLGRRAFLQRAAAGMGGAIAMPYLVPGTALGADGRT
                     AASERITVGVIGMGGRGRQVTNAFMAQPDVQVVAVCDVFRDRREAAKQQVDQYYGSSD
                     CAVYGDMLELLDRSDIDAVFIATGDNWHSEAAILAARAGKDMYCEKPLSVAITESRAV
                     ADTMRRLGRVFQCGTQRRSIGHFRFAVDLARSGKLGRLKVMEAEESTGFQQLYDSVLP
                     GEPEPPREEFDWNRWLGPAPWRPYNAQYATRGFWSAHTDFSGGSITEWGSHTVDLCQW
                     ANDADGTGPVEFDREGDRWVGKYANGVKLVIRTGLRFGSCPVRFEGDEGWVETGDSGE
                     IEVHPASLLAERQFQGGYPSNNHVRAFLDCVKTRQETISNAEVAHRSISACHVANICK
                     RLGRPVKWDPIKEEFIGDEEANRLRSRAYREPWYL"
     gene            1593..3557
                     /locus_tag="PKW60_06610"
     CDS             1593..3557
                     /locus_tag="PKW60_06610"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPJ99140.1"
                     /translation="MSLTRVFWSVVAALVFSVALSAVVSHAGEAAENAAPPAPLTEPE
                     LIAVLQSDADWAQKQEACRRLREIGTQASISALAALLPDESLSHLARFALESMPYPEI
                     DQVFRDALGTTSGLCRTGVIISIGARRDAAAVPLLLPLLTEPDVETARAAAGALGRIA
                     TPEAIETLLAYRASAPAAVSAAVNEGVLAAAEHCAAEGKASRAAEIYAGLLASDAPLA
                     VRLGAFRGLATARPATAQAQILEALRSDDAVFRDLAAQLVAETEGREETEAYARALPG
                     LSAGGQIALLRGLATRGDLAARPAAIELTKSTDTQVQVAAIKALGALGSSGDDVATLV
                     AFLGSTDEAAAEAAHGALVSMRGDAVDPALASSIAGATPAVHATLLELLANRRAEQAV
                     PSALDGLSASEADVRTAALDALGLLGGPAEAPVVIGVLTKTADLSERASAENALNAIG
                     ARKGEQILPAILEAMDGTSPDVHLTLLHAAAGIGTTRALEAVLAAMKNANPEIAGEAT
                     RILSNWTALDAAPHLEALASGKELTPYVLGLRGYVRLAQERAQGAQQQEMLGKALQLA
                     RRPEEVKLVLGAWGALPDPGALTTLMPYLDQADVRNEAALALIGIAGQVNKDNPEHKA
                     LALDALNAVLAKCDDTGIRDNAQKVIDGLQ"
     gene            3629..>6610
                     /locus_tag="PKW60_06615"
     CDS             3629..>6610
                     /locus_tag="PKW60_06615"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPJ99141.1"
                     /translation="MGCMITSFVMALTSMATGASAGCSVTDLRCEYLRDPLGVDRLHP
                     RLSWRLESAERGALQSAYQILVSDSPAVLDAGRGNLWDSGRVESSQSALVAYAGVALT
                     SRQRCYWKVRVWDASGAASEWSPAAMWTMGLLNPEDWTGKWIGLDSEEGGDASPLSES
                     KWIWFPEGAPDQSAPSEARYFRKVFEVPAGKTIAKATAFFAADNKFVLVLNEQRAASG
                     SSFQAAPPVDVTSLLRPGRNVLAVEAINEGEAANPAGLIGALIVEFEDGETQAVVTDT
                     TWRVTRDSGADWTYPELDVTAWERALELGAFGMAPWGRIASDDSPRLSARHLRREFQL
                     NKPVERATVRLCGLGLFELEVNGTKIGEDVLVPALTEYPKRAFYLTYDVTDALKPGAN
                     ALGVILGNGRYFAPRRKSPTNTLTYGYPKLMLNLRIEHPDGTVTDLDSDESWKLATDG
                     PIRENNEYDGEMYDARMELAGWSSAGYDDGGWQSANVVSGPEGALCAQMTPPIRVIET
                     RSPISVNEVSPGVFIYDMGQNMVGWCRLRVQGPAGTAVRLKHAETLQDDGTLYMANLR
                     GAKCVDTYILKGGGQETYEPRFTYHGFRFVELTGYPGVPGLDAIEGCVVHDDVERVGY
                     FKTSNALLNQLHENIVWGIRGNYRSFPTDCPQRDERQAWLGDRSEECRGESYIYNIAP
                     LYTKWLQDIDDTQKESGSLSDVSPPYWPLYNDNVTWPSTFILAPGMMLEQYGDSRPIE
                     QHYAAMTRWIDHMSGYLENDLMPRDNYGDWCVPPESPELIHSKDPSRKTSGTLLGTSY
                     FYYDLKLMVRYARMLGKEDDAARFDALASRLEKAFNEAYFNKEVNLYDNGTQTSCVLT
                     LAFGLVPEGHREAVFANLVQNIEQKTHSHVGTGLIGGQWLMRTLTDHGRVDLAYTLST
                     QKTYPSWGYMIEKGATTIWELWNGDTADPAMNSGNHVMLVGDLLTWFYGYLGGIRPTP
                     DAPGFQSASIRPYLAGDLTTVD"
BASE COUNT         1266 a         1871 c         2308 g         1165 t
ORIGIN      
        1 tcgcaaatgg caggacgata ccatctccgg taagatctta gatggcgcga ttcggaacct
       61 tgatacaggt agcgcgcggg ggtatgcttt tcgttggact gtttgaatcg cggaattgcc
      121 tgccattcca ataggacctc agcatgggta cggctggatc cgtgtcagtg cgttccgggc
      181 gttactgcgc tgagataacg tgttcgcgca gaaattccag ccattttcag gagaacacag
      241 catgagtcga gcaaaaaaaa gtcagcaatt gggtcggcgc gcttttttgc agcgggccgc
      301 ggctggaatg ggcggcgcca tcgcgatgcc gtatctggtt ccaggcacgg cgctgggtgc
      361 ggacgggcgc acggcagcca gtgaacgaat cacggtgggt gttatcggca tgggcggccg
      421 tggaaggcag gtgaccaacg cattcatggc gcaaccggat gtgcaggtgg tcgcggtatg
      481 cgatgtgttt cgagaccggc gcgaggccgc aaaacagcag gtggatcagt actacggcag
      541 ctcggactgc gcagtgtacg gggatatgct ggagctgctg gaccggagcg acattgatgc
      601 ggtgtttatc gccaccggag ataattggca ttcggaagcg gcgattctgg cggcgcgggc
      661 gggaaaagac atgtactgcg agaagccgtt gagcgtagcg atcacggaat cgcgcgcggt
      721 tgcggatacg atgcgccgcc tgggacgcgt atttcaatgc gggacgcaac ggcgcagcat
      781 cggccacttc cgttttgccg tggacctggc gcgcagcggc aaactgggcc gtttgaaggt
      841 gatggaggcg gaggagtcga ccggctttca gcagctctat gattcggtgc tgccaggaga
      901 gccggagccg ccccgcgagg aatttgactg gaaccggtgg ctgggtccgg cgccgtggcg
      961 cccgtacaac gcgcaatacg caacccgggg gttctggagc gcgcataccg atttcagcgg
     1021 cggttcaatc acggagtggg ggagccacac ggtcgacctt tgtcaatggg caaacgatgc
     1081 ggacggcacc ggccccgtgg agtttgatcg agaaggcgat cgctgggtgg gcaagtacgc
     1141 gaacggggtg aaactggtga tccggaccgg actgcggttc ggttcgtgcc cggtgcgctt
     1201 tgaaggcgac gaaggctggg tggagacggg cgattccggc gagatcgagg ttcatcccgc
     1261 gtccctgctc gccgaacgcc agttccaggg cggatatcct tccaacaacc acgtgcgagc
     1321 cttcctcgac tgcgtcaaga cgcgccagga gaccatatcc aatgcagagg tcgcccaccg
     1381 gtcgatttca gcgtgtcacg tggcaaatat ctgcaaacgg ctcgggcgtc cggtgaaatg
     1441 ggacccgatc aaggaagaat ttatcggcga cgaggaagcg aaccgtctgc gttcgcgggc
     1501 ctaccgtgag ccgtggtacc tgtaaacgaa cgtgggtcaa agctcgagtg cgagttcatt
     1561 tgcacgggct ttcagggaag aggcatcgaa tcatgagtct aaccagagtg ttttggagtg
     1621 ttgttgccgc gctcgtcttt tcagtggcac tgtccgcggt ggtgagccat gccggcgagg
     1681 cggcagagaa cgcggcgcct cccgcgccgt taacagaacc cgagctgatt gcagtcctgc
     1741 aaagcgacgc ggactgggcc cagaaacagg aggcctgccg gcgcttgcgt gaaatcggga
     1801 cgcaggcgtc gatttctgcg ctggcggccc tgctgccgga cgaatcgctg tcgcatctcg
     1861 cgcggtttgc cctggaatcc atgccgtacc cggagatcga tcaggtcttc cgtgatgcgc
     1921 tcggcacgac gtccggtctg tgccggacgg gcgtgatcat ctcgattggc gcacggcgcg
     1981 atgcagccgc ggtgccgctc ctgctcccgt tgctgactga gccggatgtg gagacggccc
     2041 gcgcggcagc cggcgccctg gggcgcatcg cgacgccgga ggccatcgag acgctgctcg
     2101 cgtaccgggc cagcgccccg gccgcggtca gtgctgcggt gaatgagggt gtgcttgccg
     2161 ctgccgagca ttgcgccgcc gagggcaaag ccagtcgcgc agcggaaatt tacgcgggat
     2221 tgctggcatc ggatgctccg ctggcggtgc gcctgggggc gtttcgtggc ctggccaccg
     2281 cgcgccccgc gactgcacag gctcagattc tcgaggcgct gcggagcgat gatgcggtat
     2341 ttcgcgatct ggctgcccag ctcgttgcgg agacggaagg ccgtgaggag acggaagcct
     2401 atgcccgcgc cttgccgggc ctttccgcgg gcggacagat tgccctgctg cgcggcctcg
     2461 cgacccgggg cgatcttgcg gcccgcccag cggcaattga attgacaaag agcacggaca
     2521 cgcaggtgca ggtggcggcc atcaaagcgc tgggcgccct gggcagcagc ggggacgatg
     2581 tggccacgct ggtcgcattt ctgggttcga ctgacgaggc ggccgccgag gccgcgcatg
     2641 gcgcgctggt ctcgatgcgt ggggatgcag tggatccggc gctggcatcg agtatcgcag
     2701 gcgccacgcc ggcggttcat gcaaccttgc tggaactgct ggcaaaccgc cgcgcggagc
     2761 aggcggtccc gtcggcgctg gacgggctgt cggccagcga agctgatgtt cgcaccgcag
     2821 cgctggatgc gctggggctc ctgggcggtc cggccgaggc tccggtggtc atcggagtgc
     2881 tcacgaagac ggcggatttg tcggaacgcg ccagtgcgga gaatgcctta aacgcgatcg
     2941 gcgcgcgcaa aggcgaacag atattgccgg cgatactcga ggcaatggat gggaccagcc
     3001 ccgacgtcca cctgacgctg ctccatgcgg cggcggggat cgggacgacc cgggcgcttg
     3061 aggccgtttt ggcggcaatg aagaacgcca atccggaaat agcgggcgag gcgacgcgca
     3121 ttctctccaa ctggacagcg ctggacgcgg cgcctcatct cgaagcactt gccagcggca
     3181 aggaattgac gccgtacgtg ctggggttgc gaggctacgt ccggctcgcc caggaacggg
     3241 cccagggcgc ccagcaacag gagatgctcg gaaaggcatt gcagctggcg cggcggccgg
     3301 aagaagtgaa gctcgtactg ggcgcctggg gcgccctgcc cgacccgggc gcgctgacca
     3361 cgctgatgcc gtacctggat caggcggatg tgcgcaacga ggccgccctg gcgctgattg
     3421 gaatcgcggg ccaggtcaac aaagacaatc cggaacacaa ggcattggcc ctcgatgcgc
     3481 tgaatgccgt gctggcgaaa tgcgacgaca cgggtatccg cgacaatgcg cagaaagtga
     3541 ttgacgggct gcaatgaatc gagacctggg ttttcaggtt tgaagcaggc ggctgtttgc
     3601 atcgcgattg cggtgaagga ggagtcccat gggctgcatg ataacgtcat ttgtgatggc
     3661 gcttacttca atggcgaccg gtgcttcggc gggttgttcg gtcacggatc tgcgctgcga
     3721 gtacctgcgc gatcccctcg gcgtcgaccg gcttcatccg cgcctgagct ggcggctgga
     3781 atcagcggaa cggggcgcac tgcaaagcgc ctaccagatt ctcgtatcgg attcgccagc
     3841 agtgctcgat gccggccggg gcaacctgtg ggactcgggc agggtggaga gcagccagtc
     3901 ggcgcttgtc gcgtatgcgg gcgtcgcgct cacgtcgcgg cagcggtgtt actggaaggt
     3961 gcgggtatgg gacgcgtcag gcgcggcgtc ggaatggagc cccgctgcaa tgtggaccat
     4021 gggcctgctg aatccggaag actggacggg gaaatggatt ggactggaca gcgaagaagg
     4081 cggggatgcg tcgccgctgt ccgagtcaaa atggatctgg ttccctgaag gtgcgccgga
     4141 ccagagcgca ccgtcagaag cacggtattt tcggaaagtg tttgaggtgc cggctggaaa
     4201 gacgatcgca aaagcgacgg catttttcgc agcggacaac aagttcgtat tggtgctgaa
     4261 cgagcagcgg gccgcatcgg ggagcagttt ccaggcggcg ccgcccgtgg acgtgaccag
     4321 cctgctgcgt ccgggcagga acgtgctggc cgttgaagcc ataaacgagg gcgaggccgc
     4381 gaatcctgcc ggcttgatcg gggccctgat cgtcgaattt gaggacggag agacgcaggc
     4441 ggtggtcacc gataccacat ggcgtgtgac acgagactcg ggagccgact ggacgtatcc
     4501 ggagctggac gtgacggcgt gggaacgcgc cctggagctg ggggccttcg gtatggcgcc
     4561 gtgggggcgg atcgcgagcg atgactcgcc gcgcctcagt gcccgtcacc tgcggcgcga
     4621 atttcagctg aataaaccgg tcgaacgggc aacggtgcgg ctttgcggac tgggactctt
     4681 cgagctggaa gtgaacggca cgaagatcgg agaggatgtc ctcgtcccgg cgcttaccga
     4741 atatcccaag cgcgcgttct atctcaccta cgatgttacg gacgcgttga agccgggcgc
     4801 caacgcgctg ggcgtgatcc tgggcaacgg ccggtatttt gcgccgcgcc ggaaatcccc
     4861 cacaaatacg ctcacttacg gctacccgaa acttatgctg aatctgagaa ttgagcatcc
     4921 ggacgggacg gtgaccgatc tggactcgga tgagtcctgg aagctcgcca cggacggccc
     4981 gatccgcgag aacaacgaat atgacggcga aatgtacgat gcgcggatgg agctggccgg
     5041 atggtcgagt gcgggttacg acgacggcgg ctggcaatcc gcaaacgtgg tttcggggcc
     5101 ggaaggtgcg ctttgcgcgc agatgacgcc gcccatccgg gtgatcgaga cgcgttcgcc
     5161 catcagcgtc aatgaggtct cgccgggcgt ctttatctac gacatgggcc agaacatggt
     5221 gggatggtgc cgtctgcggg tgcaggggcc ggcgggcacc gcggtccgcc tgaaacatgc
     5281 ggagacgctg caggacgacg gtacgctcta catggcgaat cttcggggcg ccaaatgcgt
     5341 ggatacgtac atcctcaaag ggggcggaca ggaaacttac gagccgcgct ttacgtacca
     5401 cgggttccgc ttcgtcgagc tgacggggta tcccggcgtg cccggactgg acgcgattga
     5461 aggctgcgtc gtgcatgatg acgtggaacg cgtaggctac ttcaagacct caaacgcgct
     5521 cctgaaccag ctgcacgaaa acatcgtctg gggaattcgc ggcaactacc gcagtttccc
     5581 gacagactgc ccgcagcggg atgagcgcca ggcctggctg ggcgaccggt cggaggaatg
     5641 ccggggcgag agttatatct ataacatcgc gccgctgtat acgaaatggc tgcaggacat
     5701 cgacgacacc cagaaggaat cgggaagtct ttcggacgtt tcgccgcctt attggccgct
     5761 gtacaacgac aatgtgacgt ggccgagcac gttcatcctc gcgccgggga tgatgctgga
     5821 acaatatgga gattcgcgcc caatcgagca gcattacgcg gcgatgaccc gctggatcga
     5881 ccatatgagc ggctatctcg agaacgatct gatgccgcgc gacaattatg gcgactggtg
     5941 cgtgccgccg gaatcgccgg aattgatcca ctcgaaggat ccttcacgaa agaccagcgg
     6001 aacgctcctg gggaccagct atttctacta tgaccttaaa ttgatggtgc ggtatgcccg
     6061 gatgctgggc aaggaagacg atgcggcccg tttcgatgca ctggcctcgc ggctggagaa
     6121 agcgttcaac gaggcctatt tcaacaagga ggtgaacctc tacgacaatg gaacgcagac
     6181 atcgtgcgtg ctgaccctgg ccttcggcct cgtgccggaa ggccatcgcg aggccgtctt
     6241 cgcgaacctg gtccagaaca tcgaacagaa gacgcacagc catgtcggta ccgggctcat
     6301 cggcgggcaa tggctgatgc ggacgctgac cgaccacggc cgggtcgatc tggcctatac
     6361 gctgtcgacg cagaagacgt acccgagctg ggggtacatg attgagaagg gcgccacgac
     6421 catctgggag ctgtggaacg gcgacacggc ggatcccgcc atgaattcgg gcaaccatgt
     6481 catgctcgta ggcgacctcc tgacgtggtt ttacgggtac ctcgggggca ttcggcccac
     6541 gccggacgcg ccgggcttcc agtcggcaag catacgcccg tatctcgccg gcgacctcac
     6601 tacggtggac
//
LOCUS       DAOPBQ010000160         6603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10313_length_6603_cov_3.581552, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000160 DAOPBQ010000000
VERSION     DAOPBQ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10313_length_6603_cov_3.581552"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /gene="proS"
                     /locus_tag="PKW60_06620"
     CDS             complement(<1..1312)
                     /gene="proS"
                     /locus_tag="PKW60_06620"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPJ99142.1"
                     /translation="MRMSHLVGARYKERPAEATLESHAFLLRGGYARQVANGIYSLLP
                     PGFRVCHKIEQIIREEMDRAGGQEVLMPVVLPRELWEESGRYSGVGSELLRLQDRTGH
                     DMLLAMTHEEAVVHLSRNEVHSHTQFPFMLYQIQTKFRDEPRSRGGLIRVREFTMKDA
                     YSFHTSQDDLEACYARCYRAYQRIFARAGIPEVAVVESDSGMMGGSVAHEFMLLTPVG
                     EDTIVTSEDGSYVANQEVATGIIEGHLEDPLPLEKVHTPDCRSIDEVAGFLGVETRKT
                     AKAVFYDRDSEGKLVFVVVRGDREVNESKLARLIQAVPVPADDVRIRSIGAEPGFASP
                     LGIDLEKVRLIIDHTVSTSNNLVTGANERDYHYKNFNLDRDMPGIETVDVVDIEAGDL
                     APGGKSRLLLQRGIEVGNIFQLGRKYTESMGMTYDDEQGKAQVPI"
     gene            complement(1452..1853)
                     /locus_tag="PKW60_06625"
     CDS             complement(1452..1853)
                     /locus_tag="PKW60_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPJ99143.1"
                     /translation="MVTGIAHVCFRVADLEASLAFYTEQLGLTRAFDFRNDAGERTGV
                     YLHVGGRNFIELFEGVPAARDDAQSYRHLCLEVDDMKETLAALRAWGVESTEMELGGD
                     GAWQAWITDPDGNPIELHQYTDTSLQTARLS"
     gene            complement(1912..2703)
                     /locus_tag="PKW60_06630"
     CDS             complement(1912..2703)
                     /locus_tag="PKW60_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPJ99144.1"
                     /translation="MRKATQFPSRAEHVAIPVLSPKQCEIREREGRLLEVARELIIAR
                     GYHGLTMARIATRLGVAKATVYQHFSCKEEVIMALASRSVDLQRELVERAAAFQGNPR
                     ERMFAVGEATQLFAALHPDDARIFQLMNAEVITQKAAEVSLWRLRASARQTASAINGI
                     IRDAIAQGDLQLKESGAIESLTYMFWQMGESAKAAMWSWMPPGELGVANPFDAMFHSG
                     QIIGDAYGWRPLSSDWDYLSTRRRVRREVFPVECRKAYGANYLEL"
     gene            complement(3123..3974)
                     /locus_tag="PKW60_06635"
     CDS             complement(3123..3974)
                     /locus_tag="PKW60_06635"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HPJ99145.1"
                     /translation="MTQESQRRKPFHAKGMTSFIVTLGFLLLGVSGIVLYVAPRGRVA
                     HWTGWSVMGLDKETWGALHTAGAFLFLVAGALHLYYNWTTFFSYFKMRFEKGFNLKRE
                     FALALIICGLVFAGAIWNVPPVAYLAAWNEDIKDYWETVSAQPPAAHAEEWTLRQMAE
                     NRGMDLEALLDRLKEKGCEVTDSDKTIKSIAEQHGMSPNALFVLMGAPQHGGGGMGRG
                     DGMGRGGGMGRRTLEDFCSQHGIPVQDAVDRLSTAGIAADAGQTLRAIADAAGVTPAD
                     IADIAER"
     gene            4286..>6603
                     /locus_tag="PKW60_06640"
     CDS             4286..>6603
                     /locus_tag="PKW60_06640"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ99146.1"
                     /translation="MGEPSQHSRKGNTAHSPAFLSNLPFLSNPAFAIRRNLLERLRES
                     LLQKKRILLTAPPLRLSAQCQTEFAVETAYLCREDFDRIWWVPGGCPAALPGFFAEIA
                     AALRLPEAESREQARMIAAVRKHLAEEPRSLIIFDQVSDLHAVNSLLPGKISASVLIT
                     SAENLADEAYTAHEIPDFLPAESREFLTTRLGTSLTAEDDETAFHLRGVPMLLHLYAG
                     CVFMEPAKRAALRGAVERVSEDHAHRDQARTTALLEYLLRETLTSLGEHEYPAFNLFA
                     LMAFMGPQPLYANFLIDGATHLPGVLQTTLAAPGALARIVQVLSRAGVARIGPRAFSI
                     PALMQERFVLSLAGDGKAAGCEAALRFTANSFPFKEEHDGYNPQCAHLLGHASAVTTW
                     AEVMGISLEDTGRLQNQLGLYLRACDQPEDAVKYYLHAVACGEAVLGENHPKVAVRVN
                     NLGVVYRETGRLDEARQAFRRALHIIRHAYGPADPMLAMAMRNLVAVAEDSKNEFEME
                     RTYRRALRVYADSLGQAHPYVHQCLSSLSRILRKRGDAAGAARCLDEALRCAQQCEPP
                     DEEAVAVYARRLGRLLLRSGDAGRALEPLEIAVSLSRKLHGPADVSSAESLCELGRAQ
                     LQEKQLASARASFEEALQISEALRAREELQMRILGQLARVQRALDDAPGAAVSYQHIC
                     RILEATRGPQHPELAANLMRLGEVLEQTGRFQDAETCYTRVLQLETDSTSGDAGSGTL
                     HCRLGAVKRALGDYPQAMEHLKQAISADMRTSG"
BASE COUNT         1310 a         2044 c         1949 g         1300 t
ORIGIN      
        1 tgatgggcac ctgggccttg ccctgttcgt cgtcgtaggt catgcccatg gactcggtat
       61 atttgcggcc cagctggaaa atgtttccga cctcaatgcc gcgctggagc agaagccggc
      121 tctttccgcc gggggccagg tcgcccgcct cgatatcgac cacatcgacg gtctcgatac
      181 ccggcatgtc gcggtccaga ttgaaatttt tgtagtggta atctcgttcg ttcgcgccag
      241 tcaccaggtt attggaagtg ctgacggtgt gatcgatgat aaggcgcact ttctccaggt
      301 cgatgcccag aggcgaggcg aacccgggtt ccgcgccgat actccggatg cgtacatcat
      361 cggccggcac aggcacagcc tggataagcc gcgccagttt ggactcattg acctcccggt
      421 cgccgcgaac gaccacaaaa accagtttgc cctcgctgtc ccgatcgtaa aagacggcct
      481 tggccgtctt ccgcgtctcc accccgagaa atccggccac ctcgtcgatg gatcggcaat
      541 caggcgtatg gactttttcg agcggcaacg gatcttccaa atgcccttca atgattcccg
      601 tggcgacctc ttgattggcc acataggaac cgtcctcgct ggtgacgatg gtatcttcgc
      661 ccacgggcgt caggagcatg aattcatgcg ccacggagcc gcccatcata ccggagtccg
      721 actcgaccac ggccacttcc ggaatgcccg cgcgagcaaa aatgcgctga taggcgcggt
      781 aacaacgtgc atagcaggcc tccagatcgt cctgcgacgt gtggaacgag taggcgtctt
      841 tcatggtgaa ttcgcgaacg cgaatcaagc cgccccggga acgaggttca tcgcggaatt
      901 tggtctgtat ctggtacagc atgaagggaa attgagtatg gctgtgcacc tcattccggg
      961 acaaatgcac cacggcctcc tcgtgggtca tggcgaggag catgtcgtgg ccagtccgat
     1021 cctgaagacg caggagttca gaaccgacgc cgctatacct ccccgactcc tcccagagct
     1081 cgcgaggcag aacgacaggc atcagcacct cctggccgcc ggcccgatcc atttcctcgc
     1141 ggattatctg ttcgattttg tgacagacgc gaaaacccgg ggggagcagc gagtagatgc
     1201 cattggccac ttgcctcgcg taaccgccgc gcaacagaaa cgcgtgactc tcgagtgtcg
     1261 cttccgcggg ccgttctttg taccgcgcac cgacaaggtg gctcattcgc atggtgtcaa
     1321 aatctcctgt gtacgcatgt cattgtcctg ggagtacgcg ctttcgcggt ccccaaaggt
     1381 tgaaaacggg atcctacctg ccggagtgct ttccgtgcaa ggcagcaggc acggcaagcg
     1441 ctccagccgg gtcaggaaag gcgggcagtc tgaaggctcg tgtctgtata ctgatggagc
     1501 tcgatcggat tgccgtccgg atccgtgatc caggcctgcc acgcaccatc gccgcccagc
     1561 tccatctcag ttgactccac gccccacgcg cggagcgcag ccagagtctc tttcatatca
     1621 tccacttcca ggcagagatg ccggtacgat tgggcgtcat cccgcgctgc cggaaccccc
     1681 tcaaacagtt caatgaaatt gcgccccccc acatgaagat aaactcccgt gcgctctccg
     1741 gcgtcattgc gaaaatcaaa cgcgcgggtg aggccgagct gctccgtgta aaaagccagg
     1801 gaggcttcga gatctgccac acggaaacag acgtgggcaa tgccggtgac cactgcggat
     1861 tttcctcctc tgtgcggcgc gagattggcc aggacgctgc cgacggacgg cttaaagctc
     1921 taaatagttc gcaccatacg ccttacggca ctcgacggga aacacttccc tgcgcacccg
     1981 gcgccgggtg ctcaggtagt cccagtcgct cgataagggg cgccagccat aggcatcgcc
     2041 aataatctgg ccgctgtgaa acatggcgtc aaacgggttg gcaactccca actcccccgg
     2101 gggcatccag ctccacatgg cggccttggc gctttcaccc atctgccaga acatgtaggt
     2161 caggctttca atggcgccac tttctttcag ttgcaggtct ccctgggcga tcgcgtcccg
     2221 gatgatgccg ttgattgcgg aggcggtctg ccgggcagat gcacgaagcc gccataacga
     2281 cacctcagcg gccttctggg tgatcacctc ggcgttcatg agctggaaga tccgggcatc
     2341 gtccgggtga agtgcggcaa acagctgcgt cgcctctccg acggcgaaca tgcgctcacg
     2401 ggggttcccc tggaacgcgg cggcccgctc tacgagctcg cgctgcaggt caacgctgcg
     2461 gctcgcgagg gccatgatga cctcttcctt gcaactgaaa tgctgatata ccgtggcttt
     2521 cgccacgcca agacgggtag cgatacgggc catggtcaag ccatgatagc cgcgcgcgat
     2581 gatcaattcc cgggcaacct cgagaaggcg cccttcgcgt tcacgaattt cacactgttt
     2641 cggagataac acgggtatcg ccacatgctc ggctctggat gggaactgtg tcgcttttct
     2701 catcaacgtc tcgtcgtcgg aggtttagag tgagatcaac aggtccgggc acctgaggcg
     2761 cctgcggaga aaaaaatgaa actggccccc ggccattcct ggccacctct ttggcctttc
     2821 tcaacaaaaa gcgattctcg tgctgtcctg aggatgcccg agatggaacc tgccgccata
     2881 gcgcgatttt acgacgtcag gtcgactttg tcaaacctgc aagactccaa gcgcatccat
     2941 cagttaatct gttgaacaca gataatttac acactgcgtt ctctttgccg gactgcagcc
     3001 gctcgcaact acaatcaccg cacagctagt acgcgaacgg tgtgtttgat ggttgggtca
     3061 catggcgctt atgccctgcg tgcgccggtc ggagtgcgtc gagctggtcc tggctccatg
     3121 cctcaacgct cggcgatatc ggcaatatca gccggcgtca cgccggcagc gtccgcaatt
     3181 gcgcgcaagg tttgtcccgc atcagccgca atgccagccg tggaaagcct gtcgacggca
     3241 tcctgcactg ggatgccgtg ctggctgcaa aaatcctcaa gcgtccggcg gcccataccg
     3301 ccaccccggc ccatgccgtc tccccggccc atcccgccac cgccgtgctg cggtgcgccc
     3361 atgagcacaa acagggcatt gggcgacatg ccgtgttgtt ccgcgatgct tttgatggtt
     3421 ttgtcggaat ccgtgacttc gcagcctttt tccttgagcc tgtcgagtaa ggcctccaag
     3481 tccatccctc ggttttcggc catttggcga agggtccatt cttcagcgtg cgcggccggc
     3541 ggttgcgcac tcaccgtttc ccagtaatcc ttgatgtcct cgttccaggc ggcgagatac
     3601 gctacgggcg gaacgttcca gatggcgccg gcaaatacca gtccgcagat tatcagcgcc
     3661 aaggcaaact cgcgcttcag attgaatcct ttctcaaagc gcatcttgaa atagctgaag
     3721 aaggtcgtcc aattgtaata cagatgcagg gcacccgcca cgagaaaaag gaacgccccc
     3781 gcggtgtgca gcgcgcccca ggtttccttg tcaagcccca tcacggacca gccggtccaa
     3841 tgagcgaccc gtcctcgcgg cgccacatac aacacgatgc cactgacacc caggagaaga
     3901 aacccgagag tcacgataaa ggaggtcatg ccttttgcat ggaagggctt acgtcgctgc
     3961 gattcttgtg tcatgtcgat tctccttcaa acggctccat tcgtgcgcat acctctttac
     4021 gctccctcga gggggggcag cgcgtggcct tgctttgggc ctggcctgca agaactgcgc
     4081 agtccgtgaa ttctttgcag tcgatatggg ccattgtgct accgtaccca ttcatacatg
     4141 aagagtttgc aaatttcata ccgattggcg acaatctgta tgaacagagc ggccctatcg
     4201 aacgcgcggg ggcttttcga aacggcggat cctctggttg tacacgatgt gcgctgagac
     4261 cattggggaa gaacgaagcc ggaatatggg tgagccgagt caacattccc gaaaggggaa
     4321 caccgcgcac tcgcccgcgt tcttgtcgaa cctccctttt ctgtccaatc ccgcgtttgc
     4381 cattcggcgt aacctgctgg aacgcctgcg ggaatccctt ctccagaaga aacgcatcct
     4441 gctcaccgcg ccaccgctcc gcctgagcgc acaatgccag accgagttcg cggtggagac
     4501 ggcctatctg tgccgggaag actttgaccg gatctggtgg gttcccgggg ggtgtccagc
     4561 tgcactgccg ggcttctttg cggagatcgc ggcagcgttg agacttccgg aggcagaatc
     4621 ccgggaacag gcccgcatga tagcggcggt tcgaaaacat ctggccgaag agcctcgcag
     4681 cctgatcatt ttcgaccagg tgagcgacct gcatgccgtg aattcgctgt tgcccgggaa
     4741 gatttcagct tccgtattga ttacctcagc cgaaaacctc gcagacgagg cgtacaccgc
     4801 gcacgaaatc cccgatttcc ttccggcaga atcacgggag ttcctcacaa ctcggctggg
     4861 aacctcgctg acggccgaag acgacgagac ggccttccat cttcgtggcg taccgatgct
     4921 gcttcatctg tacgcgggct gcgttttcat ggaaccggcg aagcgagccg ccttgcgcgg
     4981 agcggtggag cgcgtgtccg aagaccacgc gcaccgtgac caggccagga caacggccct
     5041 tctggaatac ctgctccgag aaacccttac ttcgcttggc gagcacgaat atccggcgtt
     5101 caacttgttt gccctgatgg cattcatggg tccgcagcca ctgtacgcga acttcctgat
     5161 cgacggagcc acccatctgc ccggggtcct gcagacaacc ctggctgctc cgggcgccct
     5221 ggcgcggata gtgcaggttc tttcacgggc gggagtggcg cgtatcggcc cgcgggcttt
     5281 ctccataccc gcgctgatgc aggagcggtt tgtgctgtct ctggccggcg acggcaaagc
     5341 ggcggggtgt gaagctgccc tgcggtttac ggcgaacagc ttcccgttca aagaagagca
     5401 cgacggctat aacccgcagt gcgcccacct gctgggccac gcgtccgcag tgacaacctg
     5461 ggccgaagtc atgggtattt cgctggagga tacgggccgg ctccaaaatc agctgggatt
     5521 gtatctgcgc gcctgcgacc agcctgagga cgcggtgaaa tactatctgc acgccgtcgc
     5581 gtgcggcgag gccgtgcttg gcgagaatca tccgaaagtg gccgtgcgtg tcaacaacct
     5641 gggcgtcgtg taccgtgaga cgggccgtct cgacgaagcc cggcaggcgt tcaggcgcgc
     5701 actccacatc atccggcacg cctacggccc ggcggacccc atgctggcca tggctatgcg
     5761 aaatctggtc gcggtggcag aagacagcaa aaatgagttc gaaatggaac ggacctaccg
     5821 ccgggccctg cgcgtgtacg ccgattccct tggccaggcc cacccgtacg tgcaccagtg
     5881 cctgtcgagc ctcagccgta ttttgcgaaa gcggggcgat gcagcaggtg cggcgcgatg
     5941 cctggatgag gcgttgcgct gcgcccagca gtgcgaaccg ccggatgaag aagccgtagc
     6001 cgtgtacgca cggcgactcg gacgcctgct gctgcgctcc ggcgacgccg ggcgcgcgct
     6061 tgaacccctg gagatcgcgg tgagcttaag ccgcaagctg catggtcccg cagacgtttc
     6121 gtcggcggag agtctgtgcg agctcgggcg cgcacagctc caggaaaagc agctggcgag
     6181 tgcccgggct tcttttgaag aggcactgca gatcagcgaa gcgcttcggg cacgtgaaga
     6241 gttacagatg cgcattctgg ggcaattggc gcgggttcag cgggccttgg acgatgcacc
     6301 gggggcagca gtcagctatc aacacatctg ccggatcctc gaggccacgc gcggaccaca
     6361 acaccccgag ctggctgcca atctgatgcg tctgggcgaa gtcctggaac agaccggaag
     6421 gtttcaggat gccgagacct gctacacgcg ggttctgcaa cttgaaacgg actccacgag
     6481 cggcgacgcc ggttctggca cgctgcattg caggctgggc gccgtaaaac gggcgctcgg
     6541 agactatcca caggccatgg agcacctgaa gcaggccatc agcgcggata tgcgaaccag
     6601 cgg
//
LOCUS       DAOPBQ010000161         6601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10319_length_6601_cov_4.785060, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000161 DAOPBQ010000000
VERSION     DAOPBQ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10319_length_6601_cov_4.785060"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PKW60_06645"
     CDS             complement(<1..467)
                     /locus_tag="PKW60_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPJ99147.1"
                     /translation="MKLDFVMRLVDAHCHLEDESLYSRLDSVLRDARAAGIVRILTCA
                     VTPEQWPRSAEIARQYPEVAFALGVHPWFATEESLQQLDGLRRARELGASAIGEIGLD
                     SKVESPSMELQTAVFEAQLAIAKELALPVIIHCRGAFNELHASLKRIGAPTPAG"
     gene            complement(573..1868)
                     /locus_tag="PKW60_06650"
     CDS             complement(573..1868)
                     /locus_tag="PKW60_06650"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPJ99148.1"
                     /translation="MTTSAGRDCIMPEQLALLGGPKAAPTDPEPLIAWPLITREDEEA
                     VLEVLRSGDMSGTGISKQFEREFGAWLGVPHCLAHCNGTASLQAAMFACELGVGDELL
                     CPSMTYWGSALQTFALGATPVFVDILPDTLCIDPAGIERWISPRTKAIMAVHYAGYPC
                     DMDAILPIAEKHNLKVIEDVSHAHGTLYKGRKVGTFGHVAAMSCMSAKSFAASELGML
                     ATSDRYLYERAIAYGHYARHDDLTDPRLTPFAGYPQGGVKFRANQLASALGRSQLRHY
                     DARVAEIQKAMNYFWDLLEGVPGILAHRPPKDSGSTMGGWYAAKGLYRAEALGGLSID
                     RFCEAVRAEGADTTPGANKPMHIHPVLNECDIYGHGKPTRIANADRDLRQPPESLPVS
                     LNSYNLCFSIPWFKRYEPAIIEEQAAAFRKVAENADQLL"
     gene            complement(2042..2620)
                     /locus_tag="PKW60_06655"
     CDS             complement(2042..2620)
                     /locus_tag="PKW60_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99149.1"
                     /translation="MMMGMGVELNARDYTANSCQASLSVCVFGPEPDIRKIPELGARA
                     QNRYDFCLSRDLRSLTMQITSDEEPALFSLRDTGVAVSAWQSEGIARYAGIDRTAYFL
                     LCGALGLLQYRALALNPLLQPDDFLHGADCGCLFSTCHAKHEYALLLEQPRICGGCVQ
                     FFDCLGCEPEVGALLDVIGFIHNSQPSAVSSN"
     gene            complement(2818..3486)
                     /locus_tag="PKW60_06660"
     CDS             complement(2818..3486)
                     /locus_tag="PKW60_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPJ99150.1"
                     /translation="MNGADQPYQVLVVDDHPIVRRGFAQLIKQEPDITVCAEAEDIPE
                     ALAALEACRPDLVIVDIVLKQANGLDLLKQLPVVAPETKALVVSMHDEQIYAERVLRA
                     GARGYVMKQEADEVIVTAIRRVLQGGIYVSDQVNARILMNFAQNQARFEASPVEFLTD
                     RELEVFELIGQGNSTRQIGERLNISVKTVETYRARIKEKLRLETGAELLQTSVEWAIK
                     KSLT"
     gene            complement(3483..5906)
                     /locus_tag="PKW60_06665"
     CDS             complement(3483..5906)
                     /locus_tag="PKW60_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPJ99151.1"
                     /translation="MPDSAYSAMALGDMRPEAKGRLAESERALTADRALEAQLAMLSQ
                     AAHCASEGLCLVSEEGIIQFANLPFAKMHGAKTDSVCGRHVSDFAFPESADWPSCIAL
                     AREQGALTSHTRHRRSDGTTFSGMMRVFFAGPERRNEPFLILRIQEAPEGKRLAETLD
                     TTKVSYHDLVETAPDAIIFMDLAGKILLTNAIAAERLGFDSIGELCAERESILDLIAP
                     YDRERAVQEMAACVAQGNHYGIEYDCLAKSGEILPVETSTNVVLDHAGRPVGFISIAR
                     DIRRRRIAEQRLQRHLHVEQLLTGIATRFISLQPEQVENAIAEAIGQVGEAIGVDHIT
                     VGLLSAGQKRPLRRSDWGLAGSVPSLTDMLQRSLECFPWIMRQLRSRECIRFSRLRDL
                     PVDAQEERAFLGIGPPRSLLIAPMMSRGDLAGYVSFEVMREGHEWDEAVSTLILILAE
                     VFASAISRKQTEEALRESEERFRQLVGSSHAMFWLAAADFSRALYVSPAFAEIWKMEI
                     DRLYADPLCFLAAVHPDDRERVAKSLTDEFQGGGIEFRVVRPDGSMRWLVHKGFPIRN
                     SDGEVFRFAGFAEDVTAHKILEKEILEISSREQRRIGLDLHDGLGQHLAGILCLSRGL
                     AKTLAGKGLSEATDAAEIADLIRESIAHARALARGACPVDLEADGLANALRKLAEHTS
                     QITGIECVFESDESVLISSPDQAEHLFRMAQEAVTNAIKHAEATQIRILLMETGVGKL
                     LQIEDNGKGIAQAPAAGAGMGLRLMEHRAKLANGTFVVKRRFGAGTEVSCLFRTTPAG
                     AGTDEGSKK"
     gene            6217..>6601
                     /locus_tag="PKW60_06670"
     CDS             6217..>6601
                     /locus_tag="PKW60_06670"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99152.1"
                     /translation="MEHPTRIAGIAILLTLPIMLFGCPGAPNIGGNETVALIVEPTVL
                     EFGATATELTFQVKRNYGPSSPALFRAIAMENWIAVNPPNGATSGPQDSVAITVTVDR
                     SLLTAGTNSGTVRIVSEGASDKFVRV"
BASE COUNT         1302 a         2101 c         1896 g         1302 t
ORIGIN      
        1 cccgccgggg ttggcgcccc gatgcgcttc agcgaggcgt gcagctcatt gaacgcgccc
       61 cggcaatgaa tgatcaccgg caacgcaagt tctttcgcaa tggccagttg cgcctcgaaa
      121 accgccgtct gcagttccat ggacggcgat tccaccttgc tgtccagtcc gatctcgcca
      181 atagccgagg cgcccagctc ccgcgcccgg cggagtccat ccagctgctg gaggctctcc
      241 tccgtcgcaa accaggggtg gacgcccaag gcaaaggcta cctccggata ctgccgtgca
      301 atttcagccg acctgggcca ctgctccggc gtcacggcac aagtcagtat ccggacgatc
      361 ccggcggccc gggcatcgcg gagcacggag tccaaccggc tgtacagcga ttcatcttcc
      421 agatggcaat gggcgtccac aaggcgcatc acaaaatcca gtttcattgc aaatgagctg
      481 gtcacggccg gagcacaatt acttgagtca tcccggccac cgaatacctg gcttcggccc
      541 gggcggacag catcacaggc gctgcccgcc ggttacagga gctggtcggc gttttcggcg
      601 acttttcgga aagccgcggc ctgttcctcg atgatggccg gctcgtaccg cttgaaccac
      661 gggatcgaaa agcacaggtt gtagctgttc aacgataccg gcagcgattc gggcggctgc
      721 cggaggtccc ggtctgcatt ggcgatgcgc gtcggtttgc cgtggccgta gatgtcgcat
      781 tcgttcagca ccgggtggat gtgcatgggc ttgtttgccc ccggcgtggt atcggcgcct
      841 tcagcgcgca cggcttcaca gaaccggtcg atactcagcc cgcccagggc ttccgcgcgg
      901 tacaatccct tggccgcgta ccagcctccc atcgtggaac cgctgtcttt gggcgggcgg
      961 tgggcaagaa tgcccggcac gccttcgagc aggtcccaga aatagttcat ggccttctga
     1021 atctcggcca cgcgcgcatc gtagtggcgc aactgcgatc gccccagcgc actggcaagc
     1081 tggttcgcgc ggaattttac ccctccctgt ggataacccg cgaagggggt taaacgcggg
     1141 tcggtcaggt cgtcgtgccg cgcgtaatgg ccgtacgcga tggcccgctc gtacaggtac
     1201 cgatcgctgg tcgccagcat gcccagctcg ctggcggcaa acgatttggc cgacatgcac
     1261 gacatggccg ccacatgccc gaaagtcccc actttgcggc ctttgtaaag cgtcccgtgg
     1321 gcgtgcgaca cgtcttcaat tactttcagg ttgtgcttct ccgcgatggg caggatggca
     1381 tccatgtcgc acgggtagcc cgcgtaatgc accgccatga tggccttggt gcgcggcgag
     1441 atccaccgtt cgatcccggc ggggtcgatg cacagggtgt cgggaaggat gtcgacgaaa
     1501 accggtgtgg cgcccagcgc aaacgtctgc agcgccgaac cccagtacgt catgctcggg
     1561 cacagcaatt catcccccac gcccagttca caggcgaaca tggccgcctg gagcgatgcc
     1621 gtgccgttgc agtgcgccag gcaatgcggc acgcccagcc aggcgccgaa ttcacgttca
     1681 aactgctttg agatgcccgt gcccgacatg tcgcccgagc gcagcacctc aagaaccgcc
     1741 tcctcatctt cgcgagtgat gagcggccat gcgatcagcg gctccggatc cgtcggggcg
     1801 gctttagggc ctccaagtaa agcgagctgt tccggcatga tgcagtccct ccctgcggac
     1861 gtggtcattg gcggattctc cggtagcgaa ctggcgcttc acgcccgcga taacacgacc
     1921 agcgtaacac acggatccgg gcaaaccaaa aaccgggcat ttgaacgcgc tgactgaacg
     1981 gctgggcgtg ataacgcgca cctgcggcga cagaacaggc gggcgtaatg cagacgtgtg
     2041 gctaattgct ggatactgcg ctcggctggc tgttgtggat gaagccaatc acatcgagca
     2101 aggcgcccac ttcgggttcg catccgaggc agtcaaaaaa ctgcacgcac ccgccgcaaa
     2161 tgcggggttg ttcaagcagg agggcatatt cgtgctttgc atggcaggtg gaaaagaggc
     2221 atccgcaatc ggcgccatgc agaaaatcgt caggctgaag caacgggttt aacgccagcg
     2281 cccggtattg aagcaggccc agcgcgccac atagcaaaaa atacgcagta cgatcgatcc
     2341 ctgcataacg cgcgattccc tcgctctgcc aggccgatac cgccaccccg gtatcgcgaa
     2401 gcgagaagag agcaggctct tcatcgctcg tgatctgcat ggtgagggag cgcaggtcgc
     2461 gcgacaggca aaaatcgtag cggttctgcg cccgggcgcc cagttccggg atcttgcgga
     2521 tgtccggctc agggccaaac acgcacacgg acaaggaggc ctggcacgag ttcgccgtat
     2581 agtccctcgc gttcaattca acgcccatcc ccatcatcac cccgctgttc agttcccctg
     2641 gttctccaca tcctgcacac tatttaatat tttccccctt taatcgacaa tcgggcttcg
     2701 gcggcacttt gcgtagggag ttgtcttagg gttttgtagg ggattgactg acaggagccg
     2761 cgcacggata ggttgccgca aaagcgcgaa aaatgcgaat atagaaacct gtcgccgcta
     2821 ggtcagtgac ttcttaatag cccattccac actggtttgc agcaattccg cgccggtttc
     2881 caggcggagt ttttctttaa tgcgggcccg gtacgtctcg acagtcttca cgctgatgtt
     2941 caggcgctca ccgatttgcc gcgtggagtt tccctgaccg atcagttcga aaacttccaa
     3001 ctcacggtcg gtcaggaact ccaccgggct ggcttcaaag cgggcttgat tctgcgcaaa
     3061 attcatgaga atccgtgcgt tgacctggtc gctgacataa attccgccct ggaggacgcg
     3121 ccggatggcc gtgacgatga cctcgtcggc ctcctgtttc atcacgtaac ctcgcgcccc
     3181 ggcgcgcagc acacgttcgg cgtagatctg ttcatcgtgc atcgacacca ccagcgcctt
     3241 ggtttccggg gccacaaccg gcagctgttt gagcagatcg aggccgttcg cctgcttcaa
     3301 gacaatgtcc acgatgacca gatctgggcg gcaggcttcc agggcggcca acgcttcggg
     3361 gatatcctcg gcttccgcgc acacggtgat gtccggctcc tgcttgatca actgagcgaa
     3421 tccccggcgc acaatcgggt gatcgtctac caccaaaacc tggtatggct ggtccgcgcc
     3481 attcattttt tactgccctc gtccgtcccc gcacccgctg gggtggtgcg gaaaagacag
     3541 gagacttccg tccctgcgcc aaaccgccgc ttaacgacaa aggtcccatt cgccagcttg
     3601 gcccggtgtt ccatcaggcg cagccccatc cccgcaccgg ccgcgggagc ttgcgcaatg
     3661 cctttgccat tgtcctcaat ttgtaacagt tttcccacgc ccgtttccat aagcaggatg
     3721 cggatctggg tcgcttcggc gtgcttgatg gcattggtga ccgcttcctg cgccattcga
     3781 aacaggtgct ccgcctgatc gggactgctg atcagcacac tctcatcgct ctcgaaaaca
     3841 cattcaatac cggtgatctg gcttgtatgc tcggccagct tccgtaaggc gttcgccagt
     3901 ccgtccgctt ccaaatcaac cgggcaggcg ccgcgcgcaa gggcgcgtgc atgggcgatc
     3961 gactcccgga ttaggtcggc gatctcggcg gcgtcggtcg cttcggacag tccttttcct
     4021 gccagagttt tcgccaggcc ccggctcaag cagagaatac cggccagatg ttgacctaat
     4081 ccgtcgtgca ggtccaggcc tatccgccgt tgttcccggg aactgatctc gaggatttct
     4141 ttttcgagaa ttttgtgcgc agtcacatct tccgcaaacc ccgcgaaccg aaacacttcg
     4201 ccgtcgctgt tccggatggg aaagccctta tgcaccagcc agcgcatcga accatccggc
     4261 cgaaccacgc gaaattcgat tccgcccccc tggaattcat ctgtgagcga cttggccaca
     4321 cgttcccgat cgtcggggtg aaccgccgcc agaaaacaca gcggatccgc ataaagccgg
     4381 tcgatctcca tcttccatat ttcggcaaac gccgggctca catacagcgc ccggctgaaa
     4441 tccgcggccg ccagccagaa catggcatga ctgctgccca ccagttgccg aaaccgctcc
     4501 tcgctttcac gcagggcttc ttccgtctgc ttgcggctga tagcgcttgc aaacacttct
     4561 gcgaggataa gaatcagtgt ggatacggct tcgtcccatt catggccttc ccgcatcact
     4621 tcgaacgaga cgtatcccgc cagatcgccc cggctcatca tcggagcgat cagcagggag
     4681 cggggcgggc cgatgccaag gaaggcgcgc tcctcctgcg cgtccaccgg aagatcccgt
     4741 agacgcgaga aacgaatgca ctcccggctg cggagctggc gcatgatcca ggggaagcac
     4801 tcgagcgacc gctgcagcat gtccgtcagg gagggtacgg aaccggccag gccccagtca
     4861 ctccggcgca gcggccgttt ctggcccgcg ctcagcaggc cgacggtgat gtggtcgacg
     4921 ccgatcgcct ccccgacctg cccgatggcc tctgcaatcg cgttctccac ctgctccggt
     4981 tgcaggctga tgaatcgcgt agcaatgccg gtgagcagct gctccacatg caaatgccgc
     5041 tgaaggcgct gttcggcgat tcgacgccgt cttatatcgc gggcgatgct gataaaaccg
     5101 acggggcggc cagcgtggtc cagcacgaca ttggtactgg tttcaaccgg cagaatctcg
     5161 cccgatttcg cgaggcaatc gtattcgatg ccgtagtggt tgccctgggc gacacaggcc
     5221 gccatttcct gtacggcacg ctcgcgatcg tagggggcaa tgagatcaag tatggattca
     5281 cgctccgcgc acaactcacc gatgctgtca aatccaagcc gttccgccgc aatcgcattg
     5341 gtcaaaagga tcttgcccgc aagatccatg aaaatgatgg catccggcgc tgtctcgacg
     5401 agatcgtggt agctgacctt cgtggtgtcc agcgtttccg caagacgctt gccttcgggt
     5461 gcctcctgta tgcgcaggat cagaaacggc tcattgcgcc tctcaggccc tgcgaaaaag
     5521 acccgcatca tgcccgaaaa tgtggtcccg tcgctccgcc ggtgccgcgt gtgggacgtc
     5581 agtgccccct gttcgcgcgc cagtgcaata caggagggcc agtctgcgga ttcgggaaaa
     5641 gcgaagtcgg acacgtgccg gccacacacc gaatcggtct tggcgccatg cattttcgca
     5701 aaaggcaggt tggcaaactg aatgatgccc tcttccgaga ccaggcacaa accctcgctg
     5761 gcgcaatggg ctgcctgtga aagcatggcc agctgggcct ccagagcccg gtccgctgtg
     5821 agcgcccgct ccgattcggc aagccttccc ttcgcctcgg gccgcatatc ccccagcgcc
     5881 atagcgctgt acgcagaatc aggcatcggt ggtttcctgt tcgtcccaat ggcccagtac
     5941 gattggccgg cgatgagcgc ctggtcacag tatacgccgg catttgcgcc atccgactat
     6001 aaaagagcgg atcataaaaa ggcaaattgc tattgcagac acctttgtat tccgtgctgg
     6061 atttccttca cggcggcgca cacgaaaccc atgcagacgc aactcatatt gacgtttata
     6121 cagccacatg gcatcatgga aaaaatgcag actgttcgaa cggtgcgcga atatggaggg
     6181 ggtcccgttt tccccataac gtaaggatct gctgccgtgg aacacccaac tcgaatcgct
     6241 ggcattgcta tcctgctcac tcttccgatc atgctcttcg gctgcccggg agcgcccaac
     6301 atcggcggca acgaaaccgt ggcccttatc gtagagccga ctgtacttga attcggcgcg
     6361 acggccacgg aattgacctt tcaggtcaag agaaactatg gccccagctc ccccgcgctg
     6421 ttccgtgcca tcgccatgga aaactggatc gcggtaaatc cgcccaacgg cgccacctcc
     6481 ggtccccagg acagcgtcgc cattaccgta accgtggacc gctcgcttct tacggcgggg
     6541 actaacagcg gcaccgtgcg gatagtctcg gaaggcgcca gtgataaatt tgtgcgagta
     6601 a
//
LOCUS       DAOPBQ010000162         6596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10327_length_6596_cov_3.961780, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000162 DAOPBQ010000000
VERSION     DAOPBQ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10327_length_6596_cov_3.961780"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..399)
                     /gene="rpsO"
                     /locus_tag="PKW60_06675"
     CDS             complement(130..399)
                     /gene="rpsO"
                     /locus_tag="PKW60_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPJ99153.1"
                     /translation="MAIAKERKTQIINDHAKHEGDTGSPEVQIALLTERINHLSEHFQ
                     THKKDFSGRRGLLKLVGQRRNLLNYVRNRDVERYRAIVKALGLRK"
     gene            complement(530..1762)
                     /locus_tag="PKW60_06680"
     CDS             complement(530..1762)
                     /locus_tag="PKW60_06680"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010631931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPJ99154.1"
                     /translation="MAKLMIENLDVKDKRVLMRVDFNVPLNDDGTVRDDTRIRAALKS
                     IQYVRDHGGMLILMSHLGRPKNPDKAESEEEKAKIIKANQKLRMNSVADRLRELVGGN
                     VTKVDAIVGPEVTQAVGAMKPGDIILLENTRFEPGETKNDETLSKNLAALGDVYVSDA
                     FGTVHRAHASTEGVTRFISRSAAGYLVAKEMEYFAKVLQSPDRPLTAVLGGAKVSDKI
                     TVIDNLLNLVDTLIIGGGMAYTFMKAQGYEIGDSLLDEGGLDVARQAMQKAKERGVEL
                     LLPVDTVVADAFDANANTKVAELGQIEPGWQGLDIGPRTLENFMAAIKKSKTVVWNGP
                     VGVFEMEPFAKGTRAIAELLANSPGITSIIGGGDTAAAVTQYGLAGKMSHVSTGGGAS
                     LEMLEGKELPGLAALSDA"
     gene            complement(1834..2829)
                     /gene="gap"
                     /locus_tag="PKW60_06685"
     CDS             complement(1834..2829)
                     /gene="gap"
                     /locus_tag="PKW60_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPJ99155.1"
                     /translation="MAIKVGINGFGRIGRNVFKLLMDDKAFEVVAINDLTDAKTLAHL
                     LKYDSVNGVYSREVKSTDNSIIVDGQEVKVTAIKNPAELPWKANGVSLVLESTGVFRK
                     KEQCLLHVEAGAKKVLLTVPPKDAIDAIIVMGVNEKTLKASDAVVSNASCTTNCLAPV
                     AKVLHERFGIKRGLMTTVHAYTNDQRVLDMPHSDLRRARAAAESIIPTTTGAARAVGK
                     VLPDLNGKLDGMAMRVPVKDGSVVDLVVELEKSATAADINAAMKAAAEGELKGILQYC
                     EDPIVSVDVIGNSYSSVFDSLCTSVLGDNFVKVVSWYDNEWGYSCRCVDLLKLMA"
     gene            complement(3197..4162)
                     /locus_tag="PKW60_06690"
     CDS             complement(3197..4162)
                     /locus_tag="PKW60_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPJ99156.1"
                     /translation="MNKGFVFWVGFGLVAAVSLLLIAKARWDADYFRGYDPALPLDAR
                     EELPESAMEYQRTEFSFAGIAGEDRVPAVLVTPRSWGGPYPCVIFLHGIGQKKEFIDE
                     IAPQFIRGGFAIVTFDQYTRGERHLPEDASKLDEALALRRRGALTVLETRRLVDYLET
                     REDIAAHRLYLVGASFGAITGSTAVAFEPRLKAGVLVYGGGNLRLLLDSPQTREFLGV
                     LAQPAVCLGACFFGPADPIRYVAQTSPRPLLFQNGTRDTLVPAASGEALFQAARDPRE
                     IIWYDSDHIGLDEAHVGVVLQDAIEWLRKQDLQVRKGVEGVSHAT"
     gene            4476..5669
                     /locus_tag="PKW60_06695"
     CDS             4476..5669
                     /locus_tag="PKW60_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99157.1"
                     /translation="MRSILTGCGLVVLAAAASVAETAYNVTVFPRESRVVVDAQTGAE
                     LRFLTTGASTDTNLYFHDRSWLSDGALILFYSDRDPGGLMGYLAETGELVTFTTADGA
                     LSGATAALTGPRFYAMRGNRVLEITVAITPSANPSEQASEVAVTERLICMLPSVKANT
                     ALNESCDGARLSVGVRGADTGDKPAILTIDVANGAIHELHRFDDPDTYHFHVQWSRTV
                     PTLLSYAGYPQRLMLIDTRDGEPRNPYQAWENELVTHESWWVKDQLLFCGGIHPKPGE
                     DSHVKVLDIHTGEVRIIGEGAWWPDGGDAEIAKRNWWHASGSEDGRWVAADNWHGDIM
                     VFEGKTSRPRLLTARHRTYGSGEHPHVGWDRAGRQVVFTSHLLGSPDVCVATIPQAWQ
                     DALPE"
     gene            5701..6078
                     /locus_tag="PKW60_06700"
     CDS             5701..6078
                     /locus_tag="PKW60_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HPJ99158.1"
                     /translation="MDINVRRVYEAPGKDDGCRILVDRVWPRGVSKEAAHVDEWHKNV
                     APTTPLRQWFGHDPEKWEEFRKRYWKELNANRDEVAPLLEKARSGRLTLVFSAKDEKH
                     NNAVVLREYLLASPKKRAAADQE"
     gene            6082..>6596
                     /locus_tag="PKW60_06705"
     CDS             6082..>6596
                     /locus_tag="PKW60_06705"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPJ99159.1"
                     /translation="MSWLVVAMAGAAFFSAPPVGEGFLLNGVTAHRGNSGEYPENTMP
                     AFESALALGADWIELDIYRTKDGKLAVLHDADTGRVGDRTVHIAEATYEELAQVDVAH
                     AFRTKHGLSVEACPPQRAPLLEEVLARVMAQRHTRVSIQPKQHIVDDAIALVKQMKAE
                     AWVGFNDGDRN"
BASE COUNT         1252 a         2000 c         2008 g         1336 t
ORIGIN      
        1 ttcaatcatg tcgctgaact ccattgggtc aaggaacgtc tctcaccccg ttccgagagg
       61 tccatctatt gattcgtttg tcttcgtacg tttttgagta aatactgagt ccgcggcgcg
      121 gcggcgcgct tacttgcgca aaccaagcgc cttgacaatc gcccggtacc gctcgacgtc
      181 cctgttacga acgtaattga gcaggttacg gcgctggccc accagtttga gcaggccgcg
      241 gcgaccggag aaatctttct tatgggtctg gaaatgctcc gacaaatggt tgatgcgttc
      301 ggtgaggagc gcgatctgca cctccggaga gcccgtgtcg ccctcatgtt tcgcgtggtc
      361 gttgataatc tgcgttttgc gttcttttgc gatagccatg gcctgtcacc tttctgtttg
      421 attcgcccgc ttccaagccc gccgccggtg ttgcgcgcgg ccgagaaccg ggtaatgtat
      481 tcaacactgc agacgggcca cccggcccgc cgcgttcatt ttgctgcctc tacgcgtcgc
      541 tcagcgccgc caggccgggc aattccttcc cttcgagcat ttcaagggaa gcgccgccgc
      601 ctgtggacac gtgcgacatc tttccggcca gcccgtactg ggtcacggcc gcggcggtgt
      661 cgccaccccc gataatgctg gtgatgccgg gagagttcgc gagcaattcg gcgatcgccc
      721 tggtgccttt tgcgaacggc tccatctcga acactccaac ggggccattc catacgaccg
      781 tcttcgattt tttgatggcc gccatgaagt tttcgagcgt cctggggccg atatccaatc
      841 cctgccaccc gggctcgatc tggccaagtt cggccacttt cgtgttcgcg ttcgcgtcga
      901 acgcatcggc cacgacggtg tccacgggaa gcagcagctc gacgccccgc tcttttgcct
      961 tctgcatcgc ctgcctggcc acgtcgaggc cgccctcatc cagaagcgag tcgccgattt
     1021 cgtagccttg cgctttcata aacgtgtagg ccatgccgcc gccgatgatc agcgtgtcga
     1081 ccaggttcag gagattgtcg atcaccgtga ttttgtcgga gactttcgcg ccgcccagca
     1141 cggccgtgag cggccgatcc gggctttgaa gcactttcgc gaagtattcc atctctttcg
     1201 ccacgagata acccgccgcg ctccggctga tgaagcgggt caccccttcg gtcgaggcgt
     1261 gtgcccggtg gacggtgccg aacgcgtcgc ttacgtagac atcgcccagt gcggccaggt
     1321 tttttgaaag cgtctcgtcg tttttcgtct cgccgggctc aaaccgggtg ttctcgagca
     1381 gaatgatgtc tcccggcttc atggcgccca ccgcctgcgt cacttccggg cctacgatgg
     1441 cgtcgacctt ggtcacgttg cctccgacca gctcacggag cctgtcggcc acggaattca
     1501 tgcgcagttt ctgattggcc ttgatgatct tggccttttc ttcctccgat tcggctttgt
     1561 cggggttttt aggccgcccg aggtgcgaca taagaatcag catcccgccg tggtcgcgga
     1621 catactggat gctcttgagc gccgcgcgga tgcgcgtgtc gtcgcgcacg gtgccgtcgt
     1681 cattcagggg cacgttgaaa tccacacgca tgaggacgcg cttgtctttc acgtcgagat
     1741 tttcaatcat cagcttggcc atggtcacac tccacgaaat tggtgcgacg ggcccgcgca
     1801 ccggacacgc gggcccgtga tgttgcttcg ttgttatgcc atcagtttca ggaggtcgac
     1861 gcaacggcag gagtaacccc actcattgtc gtaccaggaa acgaccttca cgaaattgtc
     1921 gcccagaacc gacgtgcaga gcgagtcgaa gacgctggaa tacgaattgc cgatgacatc
     1981 gaccgacacg atcgggtcct cgcagtactg caggatgccc ttgagctcgc cttccgcggc
     2041 ggctttcatg gcggcgttga tatccgcagc cgtggcgctc ttctcgagct cgaccaccag
     2101 gtctactacc gagccgtcct tcacggggac gcgcatggcc atgccgtcca gcttgccgtt
     2161 caggtccggc aggactttgc ccaccgcgcg ggcggcgccg gtcgtcgtcg ggatgatcga
     2221 ctctgcggca gcgcgggcgc ggcgcaggtc gctgtgcggc atgtcgagca cgcgctggtc
     2281 gttggtgtag gcgtggacgg tagtcatgag gccgcgcttg atgccgaacc gctcatgcag
     2341 cactttggca accggcgcca gacagttcgt tgtgcagctc gcattcgaca ccacggcgtc
     2401 gctggctttc agcgtctttt cattgacgcc catcacgatg atcgcgtcga tcgcatcctt
     2461 cgggggcacg gtcagcagca ccttcttggc gcctgcttcc acatgcagga ggcactgctc
     2521 tttcttgcgg aatacgccgg tagattccag caccagcgac acgccattgg ctttccaggg
     2581 cagctcggcc ggatttttga tcgccgtgac tttgacttcc tgcccgtcga caatgatgga
     2641 attgtccgta ctcttcactt cccgggaata tacgccgttg acggagtcgt acttgagcaa
     2701 atgggccagg gtcttcgcgt cggtcaaatc gttgatcgcc acgacctcga acgctttatc
     2761 gtccatgagg agcttgaaca cattgcggcc gatccgcccg aatccgttga tgcctacctt
     2821 aatcgccatg ggtgtcttcc tttcgtttca tttcactcgt tgcgaaactc gcgataatcc
     2881 ggctgcatgg ggccgtcccc agatcttcac ttccggccat gaccgatacc ggtaccggcc
     2941 aaagactttc gtgtatgcag atgggcgcgc tgctggaagg ttggtttcca gatgatactg
     3001 tgcacgcgca gccccgcggg gctaccgccc agccctgggg catgtccttc agacatctca
     3061 cctgatagtc aagcgcatac tatgccacag cctgaaaatc taagtcaaat ggcctcatgg
     3121 gaatgaggct tgctcttcca tgtggccgcc cgccattttc cgggactggc ggcaagcccc
     3181 cgcgccggcc cgaatttcac gtcgcatggg ataccccttc gacgcctttt ctgacctgca
     3241 ggtcctgctt gcgaagccat tcgatggcgt cctgcaggac cacgccaacg tgcgcctcgt
     3301 ccaaaccgat gtggtcgctg tcgtaccaga tgatctcccg ggggtctcga gcggcctgga
     3361 agagagcctc gccgctcgca gcgggaacca gtgtgtcgcg tgtgccattt tgaaacaaca
     3421 acggccgcgg ggatgtctgg gccacatagc gaatcgggtc ggccggtcca aaaaagcagg
     3481 ctcccagaca cacggccggt tgcgccagga cgcccagaaa ttcgcgtgtc tgcgggctgt
     3541 cgagcagcag gcgcaggttg ccgccgccgt ataccaggac gccggctttc agtcgaggtt
     3601 cgaatgcgac ggccgtggac cccgtgatcg cgccgaaact tgcgccgacc agatagagcc
     3661 ggtgcgcggc gatgtcttcg cgagtctcga gataatccac cagccggcgg gtttcgagga
     3721 ccgtcagtgc gccgcggcgc cgtaaggcca gggcctcgtc gagcttactg gcatcctccg
     3781 gaagatgccg ttccccgcgc gtgtactggt caaacgtcac tatcgcgaac ccgccccgga
     3841 tgaattgcgg ggcgatctca tcgataaatt cctttttctg gccaatgccg tgcaggaaga
     3901 tgacgcaggg gtatggcccg ccccacgacc ggggcgttac cagcactgcg ggcacccgat
     3961 cttcgccggc aatccccgca aacgagaatt ccgtgcgctg gtactccata gccgattcag
     4021 ggagttcttc ccgcgcgtcc aatggaagcg cggggtcata tccccggaaa tagtcggcat
     4081 cccagcgggc cttcgcaatg agcagcagcg acacggccgc caccagcccg aaccccaccc
     4141 aaaacacaaa acccttattc acttcacgat cctcggataa cgatcggcct cggacaggcc
     4201 gccgcgaaag ggcactatag catgagaggg gccctggatc gcaaaagacc cgcattatcg
     4261 gataaatatg attgtttcag cgggtatttt gcgtcgggcc cccgtttggc gctgcaaaaa
     4321 accggcggaa agccgacagg gcccaactcc tgcgcctgtc ttgaatttga cccttgcgga
     4381 aacaccgaag gcgaggcgcg tgttaattag gaatcggagg acgtttttac gtattatcgc
     4441 tcgcgtcaaa cgcgcggaag aaaggaaacg tcctgatgag aagtatattg acaggctgcg
     4501 ggctcgtggt attggccgcg gcagcatctg tggccgagac ggcctataac gtgactgtgt
     4561 tcccccgcga gagccgggtc gtcgtggatg cgcagacagg cgccgagctt aggtttctga
     4621 ctacaggagc gagcacggac accaacctgt acttccatga tcgctcgtgg ctgtcggacg
     4681 gggcgctgat tctgttttat tcagaccgtg atccgggcgg cctgatgggg tacctggccg
     4741 aaacgggcga gctggtaacc ttcaccaccg cggacggcgc tttgagcggc gccaccgccg
     4801 cattgaccgg cccccggttt tatgccatgc ggggcaatcg ggtactggaa ataaccgtgg
     4861 ccatcacgcc gtccgccaac ccgtcggagc aggcgtcgga agtggcggtc acggagcgcc
     4921 tgatttgcat gctaccgtcg gtgaaggcca ataccgcgct gaatgagagc tgcgacggcg
     4981 cgcgattgag cgtcggagtg cgcggagcgg acacaggcga caagcccgcc atcctgacaa
     5041 tcgatgtggc gaatggggcc atacacgaac tgcatcgctt cgacgatccc gacacatacc
     5101 actttcacgt tcaatggagc cggaccgttc cgactctgct cagctacgcg ggatatccgc
     5161 agcggttgat gctgatcgac acccgcgacg gcgagcccag aaacccgtat caggcctggg
     5221 agaacgagct ggtcacccac gaatcgtggt gggtgaagga tcagctgctg ttttgcggcg
     5281 gcatccaccc gaaaccgggc gaagattccc atgtgaaggt gctggatatc cacacgggcg
     5341 aagtccgcat catcggagag ggcgcgtggt ggccggatgg cggcgatgca gagattgcga
     5401 aacgcaactg gtggcatgcc tcgggaagtg aggacggccg ctgggtggct gctgacaatt
     5461 ggcatggcga tatcatggtg tttgagggca aaacaagccg gcccagacta ttgactgcca
     5521 gacaccggac ctacggctcg ggtgaacatc cgcacgtggg atgggaccgc gccggcaggc
     5581 aagtggtgtt cacgtcgcat ttgctgggat cgccggacgt gtgtgtggcg acgatccccc
     5641 aggcatggca ggacgcatta ccggagtgat tggggccagg cacatggaag gaggcgtgcc
     5701 gtggatatta acgttcgccg ggtgtatgag gcgccgggca aagacgacgg ctgtcggata
     5761 ctggtggatc gcgtctggcc gcggggagtt tcaaaagaag ccgctcacgt ggatgaatgg
     5821 cacaaaaatg tcgctcccac taccccgctt cggcaatggt tcggccatga tcccgaaaaa
     5881 tgggaagaat tcaggaaacg ctactggaag gaattgaacg cgaaccgcga cgaagtcgca
     5941 ccgctgctcg aaaaggcgcg ttcggggcgc ctgacccttg tattctccgc gaaagatgaa
     6001 aaacacaaca atgcagttgt gttgcgcgaa tatctcctgg caagcccgaa aaagcgcgcg
     6061 gcggctgacc aggagtaacg catgagctgg ctcgtagtgg cgatggcggg cgcggcattt
     6121 ttcagcgccc cgcccgttgg ggaaggtttt ttgcttaacg gcgtgacggc ccaccggggc
     6181 aattcaggcg agtatcccga aaacacgatg ccggccttcg agagcgccct cgctttgggc
     6241 gcggactgga ttgaactgga catctaccgc acgaaggatg ggaaactcgc ggtgctccac
     6301 gatgcggaca ccgggcgggt cggcgaccgt acagtgcaca tagccgaggc gacgtatgag
     6361 gagctggccc aggtggacgt ggcccacgcg tttcggacga aacacggcct ttccgttgag
     6421 gcctgcccgc cccagcgcgc accgctgctc gaggaggtgc tggcgcgcgt gatggcccaa
     6481 cgccataccc gggtcagcat tcagcccaag caacacatcg ttgacgacgc gatcgcgctg
     6541 gtgaagcaaa tgaaagcgga ggcctgggtg ggcttcaacg acggcgaccg caataa
//
LOCUS       DAOPBQ010000163         6582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10357_length_6582_cov_3.705378, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000163 DAOPBQ010000000
VERSION     DAOPBQ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10357_length_6582_cov_3.705378"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PKW60_06710"
     CDS             complement(<1..428)
                     /locus_tag="PKW60_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ99160.1"
                     /translation="MASNKRSNLFLLDYVGLILVLLLLIVVFSLYTENFFTAINFRTI
                     VNQTPDAVVIAVGMTFVLIIAGIDLSVGSVLGLSAAVLAVGLHRFGWPMPLAIAASLG
                     MGLLCGLLNGLMVITWRLPAFIVTLGMLEIARGGAYLVTST"
     gene            complement(476..1477)
                     /locus_tag="PKW60_06715"
     CDS             complement(476..1477)
                     /locus_tag="PKW60_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPJ99161.1"
                     /translation="MRGIIGSLKTMPVCLLAAALIALVAGCGNPQTASSPDRGGAPAP
                     AGKPRVALIMKSLANEFFLTMEEGAKAHQAAHAASYDLIAQGIKDERDVNRQVQLVEQ
                     MVAQGVDAIVIAPADSKLLVAACQKAMDAGVVVVNIDNKFDTEVLTERGIAIPFVGPD
                     NRKGARLVGDYVAARLSEGDPVAIVEGIPTAFNAIQRKLGFEDAINAAGLKIAAAQSG
                     SWETSKANQVVSAMITENPGIKAFFCANDSMALGAVAALRAAGKLDSVMVAGFDNISA
                     AQELLRKGEIVATADQHADQLAVFGIEYALKMLAGDAAPEDFETPVDLITPKSLADS"
     gene            1688..3277
                     /locus_tag="PKW60_06720"
     CDS             1688..3277
                     /locus_tag="PKW60_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPJ99162.1"
                     /translation="MSRFPFSENGQAATDCVLRVAIHGAAAAFFSTLCCLAALIATSC
                     AQKDGAGMPDNRGVATRGVLRVDLNIYNPGTIPAGIGEPNKVSAELAESWQAGRKGPR
                     IRYQPLIQTGASEGEWLKTQLIGGVAPEIINLNAEIAWPDVGKGWYVALDPFLEQPNP
                     YAEGNEHWIELFSNQDLVNAKRAPDGLLYCIPLDIVETGIFYNKDLMAQAGITEFPDT
                     WHDMLDVLDRLQAMKLVPMTSVANLGSDWGQDIIFEMLYHDILPEMDLAPSRPDAEGY
                     LGHYLEPREAGFLFTKGFFSTRDPRWREMNRLLLEWRGYWARELKNTDPMRLFLTGRL
                     AMLWDGSWSIRRMVTDPYVDFEWGIAYIPAITRETSPYASGTPATVIGGAAIQLHVTH
                     SAVLNDNLDDCIDFLMYLTAPQNIERLASEALVFIPNIRGAQMDPRLEPFNEIFQRRY
                     CAIKWLESFDGEYKKYWRRMLDYYLNGGVDLDEFLVMLEDNFAKWVEAHRGEPGWDFE
                     PLEAVWQQREANLIRELEPAR"
     gene            3303..4220
                     /locus_tag="PKW60_06725"
     CDS             3303..4220
                     /locus_tag="PKW60_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPJ99163.1"
                     /translation="MQRAVRRRRKADQPPLALIYGLLLAPAAFLVTFNYIPAFSALYH
                     AFTEWDIGGESTWVGLANFRALTGDPVFHKSIVNVAKLGIFAFATHLTVPFVVAEMIF
                     HLKSERWRYFCRVAVVLPMIVPGVVTFMLWSYIYSDAGILTELLVRLHLHDWVRGWLS
                     DPKTALWAVAFVGFPFAGGFNVLIYYAGLTNIPGSVLEAAELDGLSAPGRILRMHIPL
                     ILQQVKLLVVMTMIGVVNGFESIYILTMDGGPGYETTVPGLYMYLNGFSYQRMGLACA
                     IGLLMLLFLLAFTLSLNRFIRTEDYEPEV"
     gene            4224..5066
                     /locus_tag="PKW60_06730"
     CDS             4224..5066
                     /locus_tag="PKW60_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPJ99164.1"
                     /translation="MRAASTEKRTRQLVGVLWRAALALILLLTFFPFGFMVMTSFKDT
                     HQFYHTFWLPAWPVHLENYTGALADMQAYIANSIFVTAVSISGILVFGAIAGFLFARY
                     RFLGREYIYYGMIAMMMIPGVLLLVPSFLWVKQLRLLDSYAVMILPYISGGQILGIYL
                     LRGFFSQIDNDLFEAAQVDGAGLLRQLWHVGLPLAKPVLGVVAIVSALSVWNQFMWPL
                     VTTSSEDVMVLTVGMLRYNTRVGGQYGRMFAGYTVSAIPLGILFLLTTRVFMKGITAG
                     ALKA"
     gene            5348..6106
                     /locus_tag="PKW60_06735"
     CDS             5348..6106
                     /locus_tag="PKW60_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPJ99165.1"
                     /translation="MARVIAVANQKGGVGKTTTAINLSACLAAAGKRVLLIDLDPQGN
                     ATSGLGINKNELANTLYDALVNGAPLGGIRQKTLMDRLHLVPATRELVGAEVELVNEE
                     RREFRLHDALEPLRERYDYVFIDCPPSLSLLTVNGLSAADSILITLQCEYYALEGLSE
                     LLQTVKLIRDNLNPRLKLHGVLLTMYQHTNLANQVVADVRAHLGERVYQTIIPRNVTL
                     SEAPSFGKPVIFYDLKSVGARAYLALAREVVSRG"
     gene            6099..>6582
                     /locus_tag="PKW60_06740"
     CDS             6099..>6582
                     /locus_tag="PKW60_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPJ99166.1"
                     /translation="MAKKKKGLGRGLGALISSSVIEEDRSDDLAIQPDEPAGPRLLQL
                     NPRDLKPNPKQPRMTFNEETLEELSESIRRDGVQEPVIVRKTDDGYELVSGERRVRAA
                     VMADLETIPAVCREVSDADMLKLGLIENIQREDLNPIELARGYRQLVEQYEWTQEELA
                     D"
BASE COUNT         1270 a         1916 c         2034 g         1362 t
ORIGIN      
        1 gtgctggtga cgaggtatgc cccgccgcgg gcaatttcaa gcatgcccag ggtcacgatg
       61 aacgccggca gccgccacgt gatgaccatg aggccgttca acaggccgca gagcagcccc
      121 atgcccaggc tggcggcaat ggccagaggc atgggccatc cgaaacgatg caggcccacg
      181 gcaagcacgg ccgcgctgag ccccagcaca gaaccgacgg acaaatcgat gcctgcgatg
      241 atcaggacga aagtcatgcc gacggcgatc acgacggcgt cgggcgtctg gtttacgata
      301 gtccggaaat taatcgccgt aaagaagttt tcggtataca ggctgaacac cacgatcagc
      361 aggagcaaca cgaggatcag ccccacgtaa tcgagcagga agagatttga tcgcttgttg
      421 ctggccatcc cgcgctgttt tcccagatgt tgagccgtat ccggctgtcc agtgctcagg
      481 agtccgccag actcttgggc gtgatgagat cgaccggggt ctcgaaatct tcgggcgcgg
      541 catcccccgc gagcatcttc aaggcatact cgatgccaaa gaccgcgagt tggtcggcgt
      601 gctggtcggc agtcgcgacg atctctcctt ttcgcagaag ctcctgggcg gcggagatgt
      661 tatcgaagcc ggcaaccatc acggaatcca gtttccctgc cgcacggagc gcggcaaccg
      721 cgccgagcgc catgctgtcg ttcgcgcaga aaaacgcctt gatcccggga ttttcggtga
      781 tcatggccga tacgacctgg ttggccttgg aggtctccca gctgccggat tgcgcggcgg
      841 cgattttcag gcccgcggca ttgatcgcat cctcaaaacc cagcttgcgc tgaatggcgt
      901 tgaatgcggt cgggatgcct tccacgatgg cgacgggatc gccttcagag agcctggccg
      961 ccacatagtc accgaccagc cgcgcgcctt tgcggttgtc gggacccaca aagggaatgg
     1021 caatgccgcg ctcggtcaat acctcggtat caaacttgtt gtcgatattg acgacgacga
     1081 ccccggcatc catggctttc tggcatgcgg cgacgagcag tttggaatcg gccggcgcga
     1141 tgacgatggc atcgacgccc tgggcaacca tctgctcgac cagctgcact tggcggttaa
     1201 cgtcgcgctc gtctttgatg ccctgcgcga tgaggtcata gcttgcggca tgggcggcct
     1261 ggtgcgcttt cgcaccctct tccatggtca ggaagaactc gttggcgagg gatttcatga
     1321 tcagggccac ccgcggtttt cccgccgggg ccggggcgcc gccccggtcg ggagaagagg
     1381 cggtctgtgg gttgccgcaa cccgcgacta aggcgataag tgcagcggcc agcaagcaga
     1441 ctggcatggt tttgaggctg ccgattattc ctcgcatgat cgttccctcc aaggttgtcc
     1501 ccgacggtcg cggtcttgag cgggcgtatg ataacgcaat ttccgggccg gttaaactga
     1561 aacgccctga agcgggttca ccgccgccag cgtctctgga atgccggcgg cacttccgtt
     1621 agaatcgggt ttcccggaag cgcctgtcgc tgaatgcgcc gggagcagac ccgtaagggg
     1681 gatcattgtg agccgattcc cgttttcgga aaacggtcaa gctgctaccg actgcgtgct
     1741 gcgcgtcgcc atccatggcg ccgcggccgc ctttttctct accttgtgct gtcttgctgc
     1801 cctgatcgcc acaagttgcg ctcaaaaaga cggggctggt atgcccgata atcgcggcgt
     1861 ggcaacgcgt ggcgtgcttc gagtcgatct caacatatac aacccgggga cgatccctgc
     1921 ggggataggc gagcccaaca aggtgtcggc cgagcttgct gagtcctggc aggcagggcg
     1981 gaaagggccc cggatccgtt atcagccgct catacaaacc ggcgccagcg aaggcgaatg
     2041 gctgaagacg caattgatcg gcggcgttgc gccggagatt attaacctga acgcggagat
     2101 cgcgtggccg gatgtcggca agggctggta cgttgcgctg gatcctttcc tcgagcaacc
     2161 caacccgtat gccgaaggca atgaacactg gatcgagctt ttcagcaacc aggacctggt
     2221 gaacgccaag cgcgcgcccg acggcctgct ctactgcatc ccgctcgata ttgttgaaac
     2281 gggcatcttt tacaacaaag acctcatggc ccaggccgga atcaccgaat ttcccgacac
     2341 ttggcatgac atgctggacg tgctggaccg cttgcaggcc atgaagctgg tccccatgac
     2401 ttcggttgcc aacctgggct cggattgggg acaggacatc attttcgaga tgctgtatca
     2461 cgatatcttg cccgaaatgg acctggcgcc atcgcgtccg gatgctgaag ggtacctggg
     2521 gcattatctt gagccccgcg aagcaggttt tctgttcacc aaagggtttt tctcgacgcg
     2581 cgacccccgg tggcgggaaa tgaaccggct cctgctggaa tggcggggat actgggccag
     2641 agaattaaaa aacaccgatc cgatgcgcct gtttctgacg gggcgcctgg ccatgttgtg
     2701 ggacgggtcg tggtccatcc ggcggatggt cacggacccg tatgtcgatt tcgaatgggg
     2761 cattgcctac atccccgcca tcacccggga gaccagcccc tacgcttcgg gcacaccggc
     2821 caccgtcatc ggcggcgcgg cgattcagct tcatgtgacc cacagcgccg tgctcaatga
     2881 caatcttgac gattgcatcg attttttgat gtacctgact gcaccgcaaa atattgagcg
     2941 gctcgcttcg gaggcgctgg tatttatccc gaacattcgg ggcgcgcaga tggacccgcg
     3001 tcttgaaccg tttaacgaga tattccagcg ccgctactgc gcgatcaagt ggctggagtc
     3061 gtttgacggc gaatacaaaa aatactggcg ccgcatgctt gactattacc tcaacggcgg
     3121 ggtggatttg gatgagttcc tggtgatgct ggaagacaat ttcgccaaat gggtcgaggc
     3181 gcaccgcggc gaacccggct gggatttcga gccgctggag gccgtgtggc aacagaggga
     3241 ggcgaatctc attcgtgagc ttgagccggc acgataaggc attggcatcc ggagacggag
     3301 cgatgcagcg cgccgtccgc aggcggcgca aggcggatca gccgcccctg gcgctgattt
     3361 acggtctgct gctggcgccc gcggcgttcc tggttacttt caactacatc ccggcctttt
     3421 ccgcgctgta ccatgcgttc acggaatggg acatcggcgg cgagagcacg tgggtggggc
     3481 tggccaattt ccgtgcgctg acaggtgatc cggtctttca caaaagcatc gtcaatgtgg
     3541 ccaaactggg catctttgcg tttgcaacgc atctcacggt gccgtttgtg gtggcggaga
     3601 tgatttttca tctgaaatcg gagcgctggc ggtatttctg ccgcgtggcg gtcgttctgc
     3661 cgatgatcgt gcccggggtc gtcacgttca tgttgtggag ctacatctat tccgatgcgg
     3721 gcatcctgac ggagcttctg gtgcgacttc atcttcacga ctgggtgcgc ggctggctgt
     3781 cggatcccaa aacggcgttg tgggccgtgg ctttcgtggg atttccattc gcgggaggct
     3841 ttaacgtgct catctattac gccggactca ccaacattcc gggcagcgtg cttgaggccg
     3901 ccgaactcga cgggttgagc gcgccgggcc gcatcctgcg catgcacatc ccgctgatac
     3961 tgcagcaagt gaagctgctg gtggtgatga cgatgatcgg cgtggtgaac ggctttgaat
     4021 ccatttacat tctcacgatg gatggcgggc cgggctatga gaccacagtg ccgggtctgt
     4081 atatgtattt gaacggattc agctatcagc gcatgggact ggcctgcgcc attggcctgc
     4141 tcatgctgct gtttctgctg gcgttcacgc tgagtttaaa ccgcttcata cgaactgaag
     4201 actacgagcc ggaggtctga gccatgagag cggcatcgac ggagaaacgg acgcgccagc
     4261 ttgtgggcgt gctgtggaga gcggctctcg cactcattct gcttttgacg ttctttccct
     4321 tcgggttcat ggtcatgacg tcgttcaagg atacccatca gttttatcat acgttctggc
     4381 tgcccgcatg gccggtccat cttgagaact atacgggtgc cctggccgac atgcaggcct
     4441 acatcgccaa cagcattttc gtgacggccg tgagcatctc gggcatcctg gtcttcggcg
     4501 cgatcgccgg cttcctgttt gcgcggtacc ggtttttggg gcgcgaatac atttattacg
     4561 gcatgatcgc catgatgatg atcccgggcg tgctgctgct cgtgccgtcg tttctctggg
     4621 tcaaacagct gcggctgctg gattcatacg cggtgatgat actcccgtac atttccggcg
     4681 gacagatcct cgggatctac ctcttgcgcg gctttttcag ccagatcgac aacgatttgt
     4741 tcgaggcggc ccaggtcgac ggcgccgggc tgctgcgcca gctctggcac gtcggtctcc
     4801 ccctcgccaa acccgtgctc ggcgtcgtcg ccatcgtcag cgccctcagc gtgtggaacc
     4861 agttcatgtg gccgcttgtc acgacatcga gcgaagacgt catggtcctg accgtcggca
     4921 tgctgcgtta caacactcgg gtcggtggcc agtacgggcg catgttcgcc ggctataccg
     4981 tctccgccat ccccctgggc atccttttcc tgctcaccac gcgggtcttc atgaagggga
     5041 tcaccgcggg cgcactgaag gcatagtggt cactccactg tctcgttcaa acccgcccat
     5101 tgcagctcgt tgaggagcct tttcggcgcc gcccgcacgc cgccaaattg acttaagcag
     5161 tgcccgcccg tatgatacgg gttctttggg ggtgcgctcc ggcatagctt cgaaggccat
     5221 aagttgctct gacaaaaaaa gatagggcag gcgcccggcg cagggccggc ccgaagcgga
     5281 atctcccgcg gctcccctga gcaggcatgg tacgtcgagt tgcgttacag gtttggagaa
     5341 gggccgcatg gctcgagtta ttgccgttgc aaaccaaaaa ggcggagtgg gcaagaccac
     5401 tacggccatc aatctgtcgg cctgtctggc cgctgccggc aagagggtgc tccttatcga
     5461 cctggaccct caaggcaacg ccacgagcgg gctcggcatc aacaagaatg agctcgcgaa
     5521 cacgctctac gatgccctgg tcaacggcgc accgttgggt ggcattcggc agaagacgct
     5581 gatggaccgc ctgcacctgg ttcccgcgac gcgggaactg gtcggcgcag aagtggagct
     5641 ggtgaacgag gagcggcgcg aattccggct ccatgacgcc ctggaaccgc ttcgcgagcg
     5701 ctatgattat gtgttcatcg actgtccccc gtcgttgagc ctgcttacag tcaacggcct
     5761 gagtgccgcg gacagcatcc tgatcaccct ccagtgcgag tattacgcgc tggagggcct
     5821 gagcgaattg ctgcagacgg tgaaactcat ccgggataat ctcaacccgc gattgaagct
     5881 gcatggcgtg ttgcttacca tgtatcagca cacgaacctc gcaaatcaag tggttgcaga
     5941 cgtgcgggca cacctgggcg agcgcgtata ccagaccatc attccgcgca atgtgacgct
     6001 gagcgaagcg cccagttttg gtaaacccgt cattttttat gatttgaaat cggtgggggc
     6061 acgggcctat ctcgcccttg ctcgagaggt ggtatcgcgt ggctaagaaa aagaaaggcc
     6121 tgggcagagg cctgggcgcc ctcatttcga gcagtgtcat cgaagaagac cggtccgacg
     6181 atctggcgat acagccggat gagccggccg ggccgcgtct gctgcagctg aacccgcgcg
     6241 atctgaaacc caaccccaaa cagccccgca tgaccttcaa tgaagaaacg ctcgaggagt
     6301 tgagtgaatc gatccggcgt gacggtgttc aggaaccggt catcgtgcgc aagacggacg
     6361 atggatacga actggtcagc ggggagcgcc gggtgcgcgc cgccgtcatg gctgatctcg
     6421 agaccattcc ggcggtatgc cgcgaggtct cggatgcgga catgctcaag ctgggcctta
     6481 tagaaaacat ccagcgcgag gacctcaatc caatcgagct ggcgcgggga taccggcaac
     6541 ttgtcgagca atatgagtgg acccaggaag aactggcgga tc
//
LOCUS       DAOPBQ010000164         6536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10427_length_6536_cov_4.704830, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000164 DAOPBQ010000000
VERSION     DAOPBQ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10427_length_6536_cov_4.704830"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..495)
                     /gene="cas2"
                     /locus_tag="PKW60_06745"
     CDS             complement(151..495)
                     /gene="cas2"
                     /locus_tag="PKW60_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPJ99167.1"
                     /translation="MKQQHLQLSGYKGMWLFALFDLPTDTTEDIRNYTRFRKTLLQLG
                     FDMLQYSVYARYCADEDASKVHRKRVRQALPPKGHVRVLAVTDRQFGKMEVYMGKKRE
                     QTEQPPDQMLLL"
     gene            complement(499..1389)
                     /gene="cas1"
                     /locus_tag="PKW60_06750"
     CDS             complement(499..1389)
                     /gene="cas1"
                     /locus_tag="PKW60_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HPJ99168.1"
                     /translation="MLERIIDISQAGARLSVSDKRLVVEQGGEQKASIPLGEMGALVI
                     SHPAVVLTQPVLAGLAEAGAPVIVCDAKCLPTGMMLPMVGHHLQAERLAHQTQASAPV
                     CKQAWRQIVQAKIRAQARLLQDLHGDDRGLAALAERVKSGDPDNVEGQASRRYWPALF
                     GNPHFRRDRDLPDQNKNLNYGYAVLRAIVARAVCAAGLHPSLGIHHRNRYDAFALADD
                     LMEPFRPVVDRAVAAWLRANDPLAELSPQAKAALIQPLMACYPVQGEERSLFDIAARA
                     AVSLTRIFAGAAKNLVLPEL"
     gene            complement(1389..4598)
                     /gene="cas9"
                     /locus_tag="PKW60_06755"
     CDS             complement(1389..4598)
                     /gene="cas9"
                     /locus_tag="PKW60_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HPJ99169.1"
                     /translation="MDSVAKYTVLGLDLGSNSVGWALIEYRDGQPSALTGCGVRVFDA
                     GTEGDVESGRDSARGVERRDARQRRRQCERRGRRLENLAGLLSRNGLLPKGAMRKPAE
                     RHRLLQALDQEIDARYRNAAPPDEKTAAALDQLPYFLRSRALDHPLEPHEFGRALYHL
                     AQRRGYLSNRKSGKKDEDKGTVELNISELRERMSAAGVRTLGEFLAQVDPVNERRLRQ
                     RWTARDMYLDEFEAIWAAQQPHHPRLLSDALKKQIAEAIFYQRPLKSQKGLIGVCDLV
                     SQKKRAPKALLISQRFRMLQKVNDLEVIFADESHRRLTEDERHAVLQAMEKKDEITFP
                     QIRTAAKLPKGVEFNFQRGGEKDLPGNKTATALRAVFGEQWDRFSRETQDAIVGEAMG
                     IERAETLERRGRDAWGLDPEAAAAFGKVHLESGYMALSREAMRAILPLMEQGIQFATA
                     RDQAFEGNWARNVLDQLPGVAEACKTIRNPVVMRTLTETRRVVNAIIRTYGKPDYIRI
                     ELAREMKKSRDERKKITAEIQKRRKANDALRQKIIDTTGDPRVTGRDLLKARLWEECG
                     GLWKECGGRCPYTGKSIPFHELFSQDSPWDIEHIIPFSRCLDDSFFNKTLCYHEENRN
                     VKQNRTPREAYEHSGHWDDILDRVARFQGDAAREKLRRFQMSTEDLLEFEEFSARQLN
                     DTKFASREARNYLAWLYGPEAQSRIQASRGQITSYLRSAWRLNSILGDGGEKVRGDHR
                     HHAVDAIAIALTDRATVKALSDAAASQFRERGRSRGFEKRMTEPWAGFLEDVQRAIDG
                     VVVSHRPSRKVAGSFHEDTNYSPPRTDREGKPYVLVRKPLKKDFKAADIQNIADPTVR
                     DIVWQHFRDHDEDSDAAFSNSANHPRIRIKDGPRAGQAIPIHKVRLKYTRDVTPIGHG
                     PTERHVWTRGNSHIEIFEVLGKDGNVTKWQGRVVTRLDACQRRKQGQPIVNRHWDESR
                     RFLFSLTQRDVVEIDVETTPGGLRRELYIVRGVSMEKDGRLELMHIHDARRKADVPRS
                     ATDDEKVRIRIAPFVSSLKKRNCRKAMVTPLGEVYPAND"
     gene            5106..>6536
                     /locus_tag="PKW60_06760"
     CDS             5106..>6536
                     /locus_tag="PKW60_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPJ99170.1"
                     /translation="MIEHYFGEPDGPRGPETARERISELRSEKAQRKRAERVLRKNAE
                     FLRGILDTMALSSVFVADYNGVIIFANPRTEEITGMPLDQFVGRSCLVDDWSLCDTSG
                     RELRPEEYPFMRARDTGQRVTNEMTLLIRDDGSSRHLVVNAAPMRDEAGQITHVVIGM
                     QDITELKHTEEAYRVLVEDSAQGFSLFQDSRVVYANSALAEMADCRVDDLIGLSIEQA
                     AERYIHPEDRNATLASFQAVSGGNGAAQSGIYRLIRADGGIRHLQSYVGLTRYQGRPA
                     VQVVNVDISERMAAAKALEDSRRNFQELFNRLEDFVVVLSLEGRIVQVNPATIRRLGY
                     PEEQLLGSPVAMLYPPDRRQEAEELLTEQVAGRRLCCEMPFQAADGTVIPVDTHVSSG
                     RWNGEQALYAVSRDISERLKTEAKLRESERRFTETLQASRHVLYRFNMRTNRYDYLSP
                     HVAEFSGFSYDEAINRGLAEILQGFHP"
BASE COUNT         1105 a         1855 c         2191 g         1385 t
ORIGIN      
        1 tgtggtttgg ggagaatctc cgctcaggta tactcgcgtc ggtttcgaac tcctcatcca
       61 tctcgctgtg gtttggggag aatctccgct caggtatact attctctgta taagcccccg
      121 aaaagcgggg gcttataccc tttctggggc ttataaaaga agcatctggt cgggcggctg
      181 ctccgtctgc tcgcgttttt tacccatgta gacctccatt tttccgaact gacgatcggt
      241 gacagcgagc acgcgaacgt gtcctttggg ggggagggcc tgacggaccc gcttccggtg
      301 cactttgctg gcatcttcat cggcgcagta ccgggcatag acggagtatt ggagcatgtc
      361 aaacccgagc tggaggagcg tcttccggaa tcgggtgtaa tttcggatat cttcggtggt
      421 gtcggtgggc agatcgaaca gtgcgaagag ccacatgcct ttgtatcccg agagttggag
      481 gtgttgttgc ttcatgactc agagttcggg gaggacgagg tttttcgcgg cgcccgcgaa
      541 gatccgggtg agcgacaccg cggcgcgtgc ggcgatgtcg aacagggagc gttcttctcc
      601 ctggacggga tagcaggcca tgaggggctg aatgagcgcg gccttggcct ggggagacag
      661 ctcggccagg gggtcgttgg cccgaagcca ggcggcgacg gcgcggtcga cgacgggccg
      721 gaagggctcc atgaggtcgt cggcgagggc aaacgcgtcg tagcggttgc ggtgatggat
      781 cccgagcgag ggatggagtc cggccgcgca aacggcccgg gcgacgatgg cgcgcaggac
      841 cgcgtagccg tagttgaggt ttttgttctg gtcgggcagg tcgcggtcgc gccggaaatg
      901 cgggttgccg aacagagcgg gccagtagcg gcgggaggcc tggccttcga cgttatcggg
      961 gtcgccggac ttgacgcgtt cggccagggc cgcgaggccg cggtcgtcgc catggaggtc
     1021 ctgcaggagg cgggcctgag cgcggatttt ggcctggacg atctgtctcc aggcctgctt
     1081 gcacacgggt gcggaggcct gcgtctggtg ggcgagccgc tcggcctgga ggtgatggcc
     1141 caccatgggg agcatcatgc ccgtgggcag gcatttggcg tcgcacacga tgaccggcgc
     1201 gcccgcctcc gccagcccgg ccagcacggg ctgcgtcaac accaccgccg gatgggagat
     1261 gacgagggcg cccatctccc cgagcgggat ggaggccttc tgttcgccgc cctgttcgac
     1321 gaccaggcgc ttgtcgctca cggagagccg cgcacccgct tgcgaaatgt cgatgatccg
     1381 ctccagcatc agtcgttcgc cggatagact tcgcccagcg gcgtcaccat cgccttgcgg
     1441 cagttccgct tctttagaga cgaaacaaaa ggcgcgattc taatgcgaac cttttcatca
     1501 tctgttgcag acctgggaac gtccgccttc ctccgggcat catgaatgtg catcaattca
     1561 agtcgtccat ccttttccat ggagacacct ctcacgatgt acaattcgcg tctcaaccct
     1621 cccggcgtgg tttcgacgtc aatttccacg acgtcacgtt gcgttaatga gaagaggaag
     1681 cggcggctct cgtcccagtg gcggttgacg atgggctggc cctgtttccg gcgctggcag
     1741 gcatcgaggc gggtgaccac gcgcccttgc cattttgtga cgttgccgtc cttgcccaaa
     1801 acttcgaaga tctcgatgtg gctgttgccg cgggtccaga catggcgttc ggtcgggcca
     1861 tgaccgatcg gggtgacgtc gcgggtgtat ttgagccgga ctttgtggat tgggatggcc
     1921 tgcccggcgc gggggccgtc tttgatgcga atgcgcgggt ggttcgcgga attggagaag
     1981 gcggcatcag aatcctcgtc atggtcccga aaatgctgcc agacgatatc gcgcacggtg
     2041 ggatcggcga tattctgaat gtccgcggct ttgaagtctt ttttcagcgg cttgcgcacg
     2101 aggacgtaag gtttgccttc tctatcggta cggggcgggc tgtaattggt gtcttcgtgg
     2161 aaagagccgg cgaccttgcg cgacggccgg tgcgacacca ccacgccgtc aatggcccgc
     2221 tgcacgtctt ccagaaagcc cgcccagggc tcggtcatgc gtttttcgaa tccgcgggaa
     2281 cggccgcgtt cgcgaaactg ggatgcggcc gcatcgctga gcgccttcac ggtcgcccgg
     2341 tcggtgaggg cgatggcgat cgcgtcgacg gcatgatggc ggtggtcgcc gcgtactttt
     2401 tcgccgccgt cgcccaggat ggaattgagc cgccaggccg agcgcaggta gctggtgatc
     2461 tggccgcggc tggcctggat gcggctctgg gcttccggac cgtaaagcca cgcgaggtaa
     2521 tttcgggctt cgcgggaggc gaacttggtg tcgttgagct ggcgcgcgct gaactcttcg
     2581 aattcgagca ggtcctcggt gctcatctgg aagcggcgga gtttctcgcg cgcggcatcg
     2641 ccttgaaatc tcgcgaccct gtcgaggatg tcatcccagt ggcctgaatg ttcgtaggcc
     2701 tcgcggggcg tgcggttttg tttcacgtta cggttttctt cgtgatagca gagggtcttg
     2761 ttgaagaagg aatcgtcgag gcagcggctg aacgggatga tgtgctcgat gtcccagggg
     2821 gaatcctggc tgaaaagctc atggaagggg atggacttgc ccgtgtacgg gcatctcccg
     2881 ccgcactcct tccagagccc gccgcactcc tcccagagcc gtgccttgag gaggtcgcgc
     2941 ccggtcacgc gggggtcgcc ggtggtgtcg atgattttct gccgcagggc atcgttggct
     3001 tttcggcgct tctggatctc cgcggtgatc tttttgcgct catcacggct ctttttcatc
     3061 tcgcgggcaa gctcgatgcg gatgtagtcg ggttttccgt aagtgcggat gatcgcgttg
     3121 accacgcggc gggtctcggt gagcgtgcgc atcacgaccg ggttgcgaat ggtcttgcag
     3181 gcctcggcca cgccgggaag ctggtcgagc acgtttcggg cccagtttcc ttcaaacgcc
     3241 tggtcgcgcg cggtggcaaa ctggattccc tgctccatca ggggcaggat cgcgcgcatc
     3301 gcttcgcggg aaagggccat atagccggat tcaagatgga ccttgccgaa ggcggcggcc
     3361 gcttcggggt cgaggcccca ggcgtcgcgc ccgcgccgct ccagggtttc agcccgctca
     3421 atgcccatgg cttcgccgac aatcgcatcc tgcgtttcac gggagaaacg gtcccactgt
     3481 tcgccaaaga cggcgcgcag cgcggtggcg gtcttgttgc cgggcaggtc cttttccccg
     3541 ccgcgctgga agttgaactc tacgcctttc gggagcttgg ccgccgttcg aatttgggga
     3601 aaggtgatct cgtctttctt ctccatggcc tgaaggaccg cgtggcgctc atcctcggta
     3661 aggcggcggt gggattcgtc ggcaaagatc acctcaaggt cattgacctt ctgaagcatg
     3721 cggaagcgct gggagatgag cagcgctttc ggtgcgcgtt tcttctggga gaccaggtcg
     3781 catacgccga tcaggccttt ctggcttttg agagggcgtt gatagaaaat ggcctcggcg
     3841 atctgctttt tcagcgcatc ggagagcagt ctggggtgat gcggctgctg cgccgcccag
     3901 atcgcctcaa attcatcgag atacatgtcg cgcgcggtcc agcgttgccg gaggcggcgc
     3961 tcattgacgg gatccacctg cgcgaggaat tcgccgaggg ttcggacgcc ggcggcgctc
     4021 atgcgttcgc gcaactcgct aatgtttagc tcgacggtgc ctttgtcttc atctttcttg
     4081 cccgacttac ggttgctgag atagccgcgc cgctgcgcga ggtggtagag ggctcgcccg
     4141 aactcatgag gttcgagagg atggtccagt gcgcggctgc ggagaaaata gggaagctgg
     4201 tcgagagcag ccgcggtttt ttcgtccgga ggcgcggcat tgcggtaccg ggcgtcgatc
     4261 tcctggtcga gggcctggag gaggcggtgg cgctccgcag gcttccgcat ggcgcctttg
     4321 ggcaggagcc cgttgcggct gaggagcccg gcgaggtttt caagacggcg gccgcggcgt
     4381 tcgcactgac gccgccgttg gcgggcgtcg cggcgctcga ccccgcgcgc gctgtctctg
     4441 ccggattcga cgtcgccttc ggtgcccgca tcgaaaacac gcacgccgca gccggtcaaa
     4501 gccgacggct gcccgtctcg atattcgatt aacgcccagc cgacggaatt ggagcccaag
     4561 tcaaggccaa gtaccgtata ctttgcgacg ctgtccatag taacccccga aaactgcttt
     4621 tttgctattg acaatcagcc cctcacagtc taatataatc tatagtatac ctgagcggtg
     4681 attccatcaa atcacagtaa ggatgtttcc ttaccggaat catcacggtg ggctccgctg
     4741 cttgcagcgc ccctagcgct ccctgccgaa aggtttgggg gcttttttat ttctggccgg
     4801 attcctgggc gcttgtgagc catcacagac aggatcgccc cgctgaagtg aggatacggc
     4861 ccttgggcgg ggtgggtctt cgtatttccc tccatttggc gccagattgg ggtgcgggaa
     4921 gccgatctgt ggctgtggat cgtggtttac gtgcttttca cgccggattg tcacgatttt
     4981 ccgattggcg tgcctagtat tgatgttgta ttatcaggta ccgaaatcta gcgagattcg
     5041 ggcagggcgc gggctgggca attcggcaat tttggtgcgt ccgccgcgaa tccgaaaggt
     5101 accacgtgat cgagcattat tttggagagc ctgacgggcc tcgcggcccg gagactgcgc
     5161 gggagcgtat ctcggaactc aggtcggaaa aggcgcaacg gaaacgcgca gagcgcgtat
     5221 tgcggaaaaa cgcggaattc ctgcggggaa ttcttgatac gatggcgctt tccagcgtat
     5281 ttgtcgcaga ttacaacggc gtgatcatat tcgcgaatcc ccggacggaa gagatcacgg
     5341 gcatgcctct cgaccagttc gtgggccgct cgtgcctggt ggacgactgg agcctctgcg
     5401 acacctcggg gcgggaactg cgcccggagg agtacccgtt tatgcgggcg cgggacacgg
     5461 ggcagcgcgt aaccaatgag atgaccctgc tgatccgcga tgatggatcg tctcgccatc
     5521 ttgttgtgaa tgcggcgccg atgcgcgacg aggcggggca gattacccat gttgtgatcg
     5581 gcatgcagga catcacggaa ctcaagcaca cggaagaggc ctatcgggtg cttgtggagg
     5641 actcggcgca gggattttct ctattccagg acagccgggt ggtatatgcg aattcggccc
     5701 ttgccgagat ggcggattgc cgggtcgatg acctgatcgg cctgagcatc gagcaggctg
     5761 cggagcggta tattcatccc gaggatcgga acgccacgct ggcttcgttt caagcggtga
     5821 gcggcggtaa cggcgccgct cagtccggga tttaccggct cattcgggcc gacggcggca
     5881 tccggcatct ccagagctac gtgggtctga cgcgttacca ggggcggccc gcggtgcagg
     5941 tggtcaatgt cgatatctcg gaacgcatgg cggcggcaaa ggcgctcgaa gactcccgcc
     6001 ggaatttcca ggaactgttc aaccgcctcg aagatttcgt tgtggtgctt tcgctggagg
     6061 gccgaatcgt tcaggtgaac ccggcgacga ttcgccgtct gggctacccg gaagagcagt
     6121 tgctcggttc gcccgtcgcg atgctctatc cgccggatcg gcgtcaggaa gccgaggaac
     6181 tgctcaccga acaggtggcg ggccggcggc tctgctgcga gatgccgttt caggcggcgg
     6241 acggcacggt gatcccggtc gatacgcatg tctcgtccgg gcggtggaac ggcgaacaag
     6301 cgctttacgc ggtgtcgcgc gatatttccg agcgtctcaa gacggaagcg aagctccgcg
     6361 aaagcgaacg ccgtttcacc gagacgctgc aggcgtcccg ccatgtgctc taccggttca
     6421 acatgcgcac caaccgctac gactacctga gcccccatgt cgccgaattt tcaggttttt
     6481 catacgatga ggcaatcaac agaggcctgg cggagattct ccaggggttc cacccg
//
LOCUS       DAOPBQ010000165         6512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10472_length_6512_cov_3.297507, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000165 DAOPBQ010000000
VERSION     DAOPBQ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10472_length_6512_cov_3.297507"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..88)
                     /locus_tag="PKW60_06765"
     CDS             complement(<1..88)
                     /locus_tag="PKW60_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPJ99171.1"
                     /translation="MSKTKPLPLVAICGRPNVGKSTLFNRLVG"
     gene            complement(88..639)
                     /locus_tag="PKW60_06770"
     CDS             complement(88..639)
                     /locus_tag="PKW60_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPJ99172.1"
                     /translation="MTVANWITIARLLAIPAIAGSIAAYSAERPQWGAVALGGFIAAA
                     LSDAVDGFVARVFRQKSRLGALLDPLADKLLINVTLVFLAVNETYAHPVPRWFPVLVL
                     GRDVVISMGAFVLSDFFQSLRVRPRLTGKVTTALQLAYIIAVLLNLDLSVGLLYAATL
                     MTLVSLLDYYVEGLRQAGRKGIA"
     gene            complement(954..1346)
                     /locus_tag="PKW60_06775"
     CDS             complement(954..1346)
                     /locus_tag="PKW60_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPJ99173.1"
                     /translation="MAKPKILIVDDEPDVVTLIERTLESEGFESVAAYDGISALDIAE
                     TEEPAVILLDIMMPMMSGYEVCEQLKANPQTQGIPIICVTSAHSAEAFVRCRQAGANA
                     LLTKPFSPAELIAQIQRQLARLEEREAT"
     gene            complement(1351..1812)
                     /locus_tag="PKW60_06780"
     CDS             complement(1351..1812)
                     /locus_tag="PKW60_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99174.1"
                     /translation="MADEMFFSEDSKSLRRGKRIAPRTETSRPCLIWPEDTPEMLLRG
                     LILDMNVYGMLIRMQESLPPGLRVQVQLMRDGEFRESLTKPVAALVVRSILQDDGFVE
                     LGIQVVQETVRPETGKPFHKALQKPRLRQRGRTRMHTRHLRSPGIKRREGE"
     gene            complement(2196..2459)
                     /locus_tag="PKW60_06785"
     CDS             complement(2196..2459)
                     /locus_tag="PKW60_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPJ99175.1"
                     /translation="MNIYVGNLSYSTTENDLRQAFEAYGTVSAARVIMDRVENRSRGF
                     GFVEMPNNSEGQAAIQAMDGADLQGRTLKVNEAQPREDRRPRY"
     gene            2737..4245
                     /locus_tag="PKW60_06790"
     CDS             2737..4245
                     /locus_tag="PKW60_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99176.1"
                     /translation="MSTYPTSARTAVLCLFALSAFGAVSPFDVGTRSQLFVDQVLVDA
                     ADGVAFTLQPARLHPENPILKADQPWEGWRVELYGNVIYDEQEKLFKMWYYAEEHEEF
                     PRHGMYYATSQDGVHWDKPLLGEVKSSKYARHNVIADGILLPSVIKDVHEPDPERRYK
                     MIGHCQDAQDYGYHTWTSPDGIHWTRFSENHIVRGADVITGYYDEQRGCYVAFPKINH
                     PVRGFDRRVFWLTTSTDFSVWSEPQLVFVPDLRDDAGSLARIEEVRPILDVPDDPALI
                     RTEFYGIGAYPAESCTLAFIWVFTINNEARYGNQEGPGELQLAASRDLIHWERPFRQP
                     CVPRGKPGEWDCGFFGTQARALRVGDEIWLYYGGSNYTHGTPCLYREEGTGRGTRYTG
                     SLGLAIWDLDRFVSVDAPAEGGSLTTVPLTFGGERLEVNARVAGSLTVALCDAAGAPY
                     PGFEASDEVTGDELRHSITWGGQADVSSLQGKPVVIRFVMRDARLFAFAFRD"
     gene            4382..5365
                     /locus_tag="PKW60_06795"
     CDS             4382..5365
                     /locus_tag="PKW60_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HPJ99177.1"
                     /translation="MKSWRFHQFGDIQNLRLEEIPVPKPKPDEALVRLEYAALNPADA
                     YLIKGQYPRPGTPPFAVGRDGCGVIEEPGNSGRFQAGDVVVILRSEIGVTRDGTLAEY
                     VAAPEASLAPLPPGWTREEGAAAPLVFLTAWQALVEVAGLEPGQTVLITGASGGVGTA
                     AVMLAKSFGATVVALSRHEDKRTRLKALGADIVLDAQIDGLEMRVKKALGGGRVDAVV
                     ENLAGPYLQASVNLCRERGRIALVGLLAGISSQITVGSLLFKRVRVEGVAVGAYTPAE
                     AQEAWRQIVARLDAAEAKPPIHAIFPLESVPEAFASLERGPFGKVLVRMGA"
     gene            5367..5915
                     /locus_tag="PKW60_06800"
     CDS             5367..5915
                     /locus_tag="PKW60_06800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPJ99178.1"
                     /translation="MAVPAQTPAQAVLVRPSAGLRLSFLDAAREYRDVGEELDPAFPA
                     YWNASFEEYVSQLDDLAHGRHLPFTMVKSDTFWLLNDEDRLVGMSRLRYRLSRSLRIE
                     GGHIGYTIRPSERRKGYGTRLCALTIDEARKTGKFSRLLITCDTRNTGSAKIIEKNGG
                     LLENYVISPRSGRQISRYWVNL"
     gene            5933..>6512
                     /locus_tag="PKW60_06805"
     CDS             5933..>6512
                     /locus_tag="PKW60_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPJ99179.1"
                     /translation="MKVIVTGASKGIGRGIATVLAREGFQVGALARSSELLDELRVEI
                     EAANGVCHSAVCDLRDPQATEHAIGTLQRQLGGLDALINNAGLVVRKNAFDLSMEEWH
                     ALIDTNIHGLFYATRAVLPGMREQRRGHIINISSISGKMPLPGGSAYAATKFAVTGFS
                     LSLLQEVREYGIKVTVVYPGSVDSPSRHGAADG"
BASE COUNT         1241 a         2017 c         2021 g         1233 t
ORIGIN      
        1 tgcccacgag gcggttaaac agcgtagact tgccgacgtt ggggcgcccg catatggcta
       61 ccagaggcaa tggtttcgtt ttagacatta cgcaattccc ttgcgccccg cctgccgcag
      121 gccttcaacg tagtaatcga gcagagagac gagcgtcatg agggtggcgg cgtacagcag
      181 gcccacggaa aggtccagat tcaacagcac cgcaataatg taggccagtt gcagcgctgt
      241 cgtcactttg cccgtgaggc gcggccgaac gcgcaggctt tggaagaagt cgctcagaac
      301 aaacgctccc atactgataa cgacgtcgcg tccgagcacc agcaccggaa accagcgcgg
      361 cacagggtgg gcatacgtct cgttcactgc aagaaacacc agggtcacat tgatgagcag
      421 tttgtccgca agcggatcca gaagcgcgcc cagtcgcgat ttctggcgaa atacgcgcgc
      481 cacgaacccg tctaccgcat cggacagggc agcagcgatg aatcctccca gcgccaccgc
      541 gccccactgg ggccgttcag ctgaataggc ggcaatactc cctgcgatag cgggtatcgc
      601 cagcaatcga gcgatagtaa tccagtttgc gacggtcacg catacgcctt tgccggatga
      661 ttgccacggc gcgggccgct tccctgcatc gagcgccgcg cccgcccagc cgccggcaga
      721 tttctggttt gctgacccct gagcttgagt gcaggaagcc gtgtatgcga ccgggcttca
      781 gcccacagtt tccgatgccg gctcgcgcgc cttgacaacc gcaacccctt gatttgcgaa
      841 tgcttgcaaa acacgaagct ctgagcgtga ctatagcatc gacacggtgc atggtgcaat
      901 cagacagcgc ccggccggct cgcacggggc tggactccgg gggctgcgcg ggattatgtg
      961 gcttcgcgtt cttccaggcg ggcgagctgc cgttggatct gggcaatcag ttccgctggt
     1021 gaaaatggtt tcgtaagcag ggcgttggcg ccggcctggc ggcatcgcac aaaggcttcc
     1081 gcggaatgtg ccgacgtcac gcagatgatg ggaatgccct gagtctgggg attcgccttg
     1141 agctgctcgc agacctcata gccgctcatc atgggcatca tgatgtcgag cagaatcacg
     1201 gcgggttcct cggtctccgc gatgtcgagc gcgctgatac cgtcgtacgc cgccacgctt
     1261 tcgaaccctt ccgattcgag cgtccgctca atgagcgtca ccacatcggg ttcatcgtca
     1321 acgatgagga ttttcggctt ggccaagttc ctactcccct tcgcgccgct ttatgccggg
     1381 cgagcgcagg tggcgcgtat gcatccgcgt cctcccacgc tgtcgcaatc gcggtttctg
     1441 cagcgctttg tggaacggtt tgcccgtctc cgggcgaacc gtttcctgga cgacctgaat
     1501 accgagctcg acgaaaccat catcctgaag gatgctccgc accaccaggg cggctacagg
     1561 cttggtcagt gattcgcgaa attcgccatc ccgcatgagc tggacctgaa cgcgcaggcc
     1621 gggcggcagt gattcctgca tccggataag catcccgtat acgttcatat cgagtatgag
     1681 gccgcgcagc agcatttccg gggtatcctc aggccagatg aggcagggac ggctcgtctc
     1741 ggttcgcggt gcaatccgtt tgccgcgccg cagcgacttg ctgtcctccg agaagaacat
     1801 ctcgtccgcc atacgactct ccctggggcc ggaggcggga gggaaaagag gccggtggcc
     1861 gccctccccc ccggaacatt ccgggcgttt cctgatgcac gttaccccgt taagatagcg
     1921 caacttggca tgcttttcca gtggtccgcc ccgctccagc attttttcgg aggaaggcag
     1981 agcctgcgga ctccggcaga aaactcagtt ttcgcgatta cccgggcaaa tgcgatacga
     2041 ggcagcgccg taaacgcagc aggtcatggc ggatcgccag cgcagcaaac ggccggcagc
     2101 cgccaaaggc tgccggttgg cctcaaaaaa gacaaaccga gctccctatc ttcaggaacc
     2161 cggcgtcaag aaggttgata ctgatttagg accggctaat accgggggcg gcggtcttcc
     2221 cgcggctgcg cctcgttgac cttcagcgta cgcccctgca aatcggcgcc gtccatggcc
     2281 tggatggccg cctggccttc gctgttgttc ggcatttcaa caaacccgaa cccgcgcgaa
     2341 cggttttcga cccgatccat gatgacgcgc gcagcgctca cggttccgta cgcctcaaac
     2401 gcctggcgca gatcgttttc ggtggtcgag tacgacagat tccccacata gatgttcatc
     2461 aaggcttcct ttctcacctc atgactcgtc cggacggtcg tagcagagac agaaggcagt
     2521 tcagccctga tgcatgcagt tggaaaacgc caacgcgcgg cgggatatga aatcttgccc
     2581 cactcggtct actatccacg aactccgaaa cccacacaat tcccagactt aggatagcat
     2641 ataacgaccc gtttgccaag aggtttcatg ggtttgtata gtgccgtgcg agttctggcg
     2701 gtaaaacgat gtatccgaac tgaagggggg ccgtaaatga gcacgtatcc gacgtcagcg
     2761 cgcacggccg ttttgtgcct ttttgcgctc tccgcctttg gggcagtttc accatttgat
     2821 gtggggactc gttcgcagct ttttgtggat caagtgctgg tggatgcggc ggacggcgtg
     2881 gcgtttacac tgcaacccgc gcgccttcac cccgaaaacc ccattttgaa ggcggaccag
     2941 ccctgggaag gctggcgggt cgagctttac ggcaatgtga tttacgatga acaggaaaag
     3001 ctcttcaaga tgtggtacta cgccgaagag cacgaagagt tcccccgcca cggcatgtac
     3061 tacgctacta gccaggatgg cgtgcactgg gacaagccgc ttctcggcga ggtgaagtcg
     3121 tccaaatacg cccggcacaa cgtcatcgcc gacggcatcc tgttgccttc tgtcatcaaa
     3181 gatgtccacg agcccgaccc tgagcgccgt tacaagatga ttggacactg ccaggacgct
     3241 caggattatg gatatcacac gtggacctca cctgacggca tccattggac ccgcttcagt
     3301 gagaaccaca ttgtccgcgg cgcggacgta atcaccggct actacgacga gcagcgcggg
     3361 tgttatgtcg ccttcccgaa aatcaaccat cccgtacgcg gtttcgaccg ccgcgtgttc
     3421 tggctcacga ccagcacgga cttcagcgtc tggtcagagc ctcagctggt ttttgtgccg
     3481 gatctccggg atgatgcagg ttccctggcg cgtattgagg aagtgcgccc aattctggat
     3541 gttccagacg atccggcgct catacgcacg gaattttacg gcatcggcgc gtacccggct
     3601 gaatcgtgca ccctggcgtt catctgggtg tttaccatca ataacgaggc ccgttacggc
     3661 aaccaggagg gtccgggcga gctgcagctg gcggcctccc gtgatctcat ccattgggaa
     3721 cggcccttcc gccagccctg cgttccgcgc ggaaagcccg gcgaatggga ttgcggtttc
     3781 tttggcaccc aggcgcgggc cctgcgggtt ggcgacgaga tctggttgta ttacggcggc
     3841 tcgaactaca cccacggcac gccctgcctt taccgcgagg aaggcacggg gcgcggcaca
     3901 cgctacacgg gctcgctcgg cctcgccatc tgggacctgg accggtttgt gtcggtggat
     3961 gcccccgccg aaggcggcag cctgaccacg gtgccgctca cgtttggcgg ggaacgtctg
     4021 gaggtcaacg cgcgcgttgc gggttcgctg accgtagcgc tgtgcgacgc cgccggggcg
     4081 ccgtatcccg gcttcgaggc gtcggacgag gtgacgggcg acgagctacg ccattcgatt
     4141 acgtggggcg gccaggcgga tgtgtcgtct ctgcagggca aaccggttgt gatacggttc
     4201 gtgatgcggg acgcccggct cttcgcgttc gcgttccgcg attaacccgg cgcggacgga
     4261 cgcctggact gcccgcgtct gaggaatagg cgggcggggc ccgtccgttg actgaagttg
     4321 tccgggttga ccggcaccgg gccccggcgc aaaatgcagc atggagggag gaatagcgca
     4381 catgaagtcg tggcggtttc atcagtttgg cgacattcaa aacctccgac tggaagaaat
     4441 tcccgtcccg aagcccaagc cggacgaggc cctggtccgc ctggagtacg ctgcgctgaa
     4501 tccggcggat gcatacctca tcaaaggcca gtatccccgc cccggcacgc ctcctttcgc
     4561 cgtcggcaga gatggctgcg gcgtgatcga ggaacccggg aacagcggcc ggttccaggc
     4621 gggcgacgtg gtggtgatat tgcgcagcga gatcggggtc acgcgcgacg gtaccctggc
     4681 cgaatacgtg gcagcgcccg aggcgtcgct ggcgcccctg cctccgggct ggacgcgcga
     4741 agaaggcgcc gcagccccgc tggtatttct gaccgcgtgg caggcgcttg tcgaagtggc
     4801 tggtctggaa cccggccaga ccgtgctgat caccggcgcc tcaggcgggg tgggcacggc
     4861 ggcggtaatg cttgcgaaat cctttggcgc gacggtcgtt gcgctgtcgc gccatgaaga
     4921 caagcgcacc cggctcaaag cgctgggagc ggatatcgtc ctcgacgcgc aaatcgacgg
     4981 cctcgagatg cgggtgaaaa aggcgctcgg aggcgggcga gtggacgcgg tcgtcgaaaa
     5041 cctcgccggc ccctacctgc aggcgagtgt caacctgtgc cgtgagcgcg gccggatcgc
     5101 gctggtgggc ctgttggctg gaatttcatc ccagatcacg gtggggagcc ttttgttcaa
     5161 gcgggtacgc gtcgaaggcg tcgcggtggg cgcatacacc cccgcggaag cgcaggaagc
     5221 ctggcggcag atcgtggcgc gcctcgacgc ggcagaggca aaacctccga tccatgccat
     5281 atttcccctg gaatctgtgc cggaagcgtt cgccagcctg gagcggggac ctttcgggaa
     5341 agtcctggta cggatgggcg cctgacgtgg cggtgccggc ccagactccc gcacaggcgg
     5401 tgctggttcg ccccagcgcc ggccttcgcc tgtcgtttct cgatgcggca cgcgagtatc
     5461 gcgacgtggg cgaagaactg gatcccgctt tccccgcata ctggaatgcc tcttttgaag
     5521 agtacgtcag tcagctcgac gatctggccc atggaaggca ccttcccttt accatggtga
     5581 aatcggacac cttctggctg ctgaatgacg aggaccggct ggtgggaatg agccgtttgc
     5641 gctaccgtct gtcacggtcg ctgcgtatcg agggcggcca tatcggatac accattcggc
     5701 cctccgaacg gcgcaaaggc tacggaaccc ggctctgtgc gctcaccatc gacgaagcgc
     5761 gtaaaacggg caaattctcg cggcttctca tcacctgcga tacccgcaat accggctcgg
     5821 ccaaaatcat cgaaaagaac ggtggactcc tcgaaaatta tgtcatttct ccccggtcgg
     5881 gccggcagat ctcccggtac tgggtgaacc tgtaaccatc aaggagcaac ccatgaaggt
     5941 catcgtaacc ggcgccagca agggcatcgg acgcggcatc gccacggtgc tggccaggga
     6001 aggtttccaa gtcggcgcgc tcgcccggtc cagcgagctg ctggacgaac tgcgggtgga
     6061 gattgaggcc gccaacggcg tctgccacag cgccgtatgc gatctccgcg atccccaggc
     6121 cacggaacac gcgatcggga ccctgcagag acagctcggc ggcctggatg ccctcatcaa
     6181 caacgcgggg ctcgtcgtcc ggaagaacgc attcgacctt tcgatggagg aatggcacgc
     6241 gctgatcgac accaatatcc acggcctgtt ttatgccacc cgggccgtat tgcccggtat
     6301 gcgcgagcaa cggcggggcc acatcatcaa catctcctcg atctcgggca agatgccgct
     6361 gcccggcggc agcgcctacg ccgccaccaa gtttgccgtc accggattct cattatcgct
     6421 cctgcaggaa gtccgtgaat acgggatcaa agtcacggtg gtctaccccg gttcggtgga
     6481 ctcgccatcg cgccatggcg cggcagatgg cg
//
LOCUS       DAOPBQ010000166         6507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10484_length_6507_cov_3.872133, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000166 DAOPBQ010000000
VERSION     DAOPBQ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10484_length_6507_cov_3.872133"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PKW60_06810"
     CDS             <1..812
                     /locus_tag="PKW60_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPJ99180.1"
                     /translation="PREVIPIWDGAPPGSTGSGEADIPTLGLYPLDTDRAAPAVLVCP
                     GGGYGGHAMDHEGEQIAQWFNANGLGAFVLKYRLSPYRHPVPLEDAKRAMRLVRANAA
                     AWNVDLARLGVMGFSAGGHLASTLSTHYEPGNATAADAIDRQSSRPDFSILCYPVITF
                     EPPYTHLGSRKNLLGEDPPEELIKNLSNHTQVTAQTPPAFLFHTADDPVVPVQNSLLY
                     FGALCEHEVPAELHVYEHGAHGVGLAPDLPDLNTWPGLCISWLRVRGILQK"
     gene            complement(842..1327)
                     /gene="ispF"
                     /locus_tag="PKW60_06815"
     CDS             complement(842..1327)
                     /gene="ispF"
                     /locus_tag="PKW60_06815"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPJ99181.1"
                     /translation="MRIGQGYDLHRLEPGRPLILGGVHVPHDKGLTGHSDADALAHAI
                     TDALLGAVSLGNIGEHFPDTDPRYKGADSLQLLAKAYFMVKELGYCLGNIDATIIAQK
                     PKLNPHIPAMRNALASCLRVPVENISVKAKTNESLGAEGREEAVSVHAVALLLRSEHS
                     R"
     gene            complement(1327..2016)
                     /gene="ispD"
                     /locus_tag="PKW60_06820"
     CDS             complement(1327..2016)
                     /gene="ispD"
                     /locus_tag="PKW60_06820"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPJ99182.1"
                     /translation="MNVRLVVAAAGMGLRLGLEQPKALALCGGKPLLIRTLARFDAAG
                     LLSRAVITVPPGAESAFEAILHNSFAGNDFVLVTGGKERQESVERALDQLDQSDEIVV
                     IHDAARPFVSHESITDSIEAAAEYGAATVAIPCADTILAGGADDMLEDTPDRRRLWAC
                     QTPQTFRVEVIKQAHRYARSQGFAGTDDATLVRRMGAAVKLVRGTPLNFKVTTPDDLA
                     LAEAVVTKGLA"
     gene            complement(2013..3083)
                     /locus_tag="PKW60_06825"
     CDS             complement(2013..3083)
                     /locus_tag="PKW60_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99183.1"
                     /translation="MIKAVRIIFVIACIIMGTLWAVLLVDNWNDYAMEQNTRLAQDVI
                     QLQDEVPWGIFGGLMGGVISVGVILALRFVTQEVFEKIAPAALCIVLGMVVGYGLVKY
                     ITHFAKPTDPNVTLFMYTSVILIFGFVGISLGLTMASNWESLKKAMHRRQFGVGQAKV
                     VDTSVIIDGRIADICKTGFIEGPLLIPRFVLKELQNIADSPDVLRRARGRRGLDIIKD
                     MQENNHLVEIQVLEDDPVEITEVDSKLVRVARDYGSKILTNDLNLNKVAQIEGVQVLN
                     INDLANALKPAVLPDEQMQVRIVKEGKEAFQGVGYLDDGTMIVVDGGKEHVGKDVSVV
                     VTSVLQTTAGRMIFTKLQSVLS"
     gene            complement(3204..4031)
                     /locus_tag="PKW60_06830"
     CDS             complement(3204..4031)
                     /locus_tag="PKW60_06830"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPJ99184.1"
                     /translation="MSELRQRIDEAVSSIRAECALEPGIGIILGTGLSKLVERIKIDA
                     RLSYDRIPHFAVSTVESHDGELLFGRLSGKSVVAMSGRFHRYEGYSLNQVTFPVRVMR
                     ALGARVLVAFNAAGGLNPLFRAGDLMVIDDHINLMGDNPLIGANDDSLGPRFPDMSEP
                     YSRDLVSLAETVALEKGIKLQKGVYLACSGPCLETRAEYRMMRGMGADVVGMSTVPEV
                     IVAVHAGMDVLAFSVITDECFPDALKALQIEKIIATASGAEPRLTELVSGCIERMSV"
     gene            complement(4064..4369)
                     /locus_tag="PKW60_06835"
     CDS             complement(4064..4369)
                     /locus_tag="PKW60_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPJ99185.1"
                     /translation="MAADAITEKDGRVYLAVRVQPKASREGVRFEADGRIRIVLTAPP
                     VDGEANDALKTFLSKRLGIPRRAIEIASGEKSREKRIVLEGITRKAVEACLRGTSKT"
     gene            complement(4372..5340)
                     /locus_tag="PKW60_06840"
     CDS             complement(4372..5340)
                     /locus_tag="PKW60_06840"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HPJ99186.1"
                     /translation="MKRGFIELVDNARSTVDPWLPFEAHSVELETRLRAAATEEERAA
                     LQRELDAELKRVFSELTPWQRVQVARHPLRPRMLDYTSRVFDDFIELHGDRAVGDDPA
                     LVGGLGRFEGRTVVVVGNQKGVSTDERLRRNFGMAHPEGYRKALRLFHLAERLQYPVI
                     TFVDTPAAHPGIEAEQRGQGPAIAENLRAMLALKTPVFSVVLAEGGSGGALGIALGDV
                     VAMFEWSIYVICPPERCAEILWRDAGRKELAASALRITADSLRNLGVIDEILPEPPGG
                     AHRDPDGAANTLRTHIRAFLEGCDANRWSPRKRQEKFRALGQWLEG"
     gene            5627..>6507
                     /locus_tag="PKW60_06845"
     CDS             5627..>6507
                     /locus_tag="PKW60_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99187.1"
                     /translation="MAMKESSTDPKARCPHFGSCGGCEFQDIAYPEQLARKSSALSAL
                     FDFCWPHPIPVEASPVVWHYRNKVDPAFGGKHYDEPPPKGFQRETVLGFKPRGRWFGP
                     IEIRECHIGPEGLTPLLGSVQAWQRAAGYRYYDTRSGSGFLRVLLVRDAKRTGQRMVV
                     LVTAEGDLDAASFVDCVQSAFPSDSIQHAVFRGRAQIAAADELRVLYGASDITEILHV
                     SDGDAVPARRLSLRVSPFSFLQTNTLAAERLYAMIRAWTKQAAPSTLHDLYGGMGSIV
                     FACADLAGEVHSVENVP"
BASE COUNT         1278 a         2075 c         1807 g         1347 t
ORIGIN      
        1 aaccccggga ggtcattccg atctgggatg gcgccccgcc gggatccacg ggctcgggcg
       61 aggccgatat cccgacgctg ggcctgtatc cgctggacac tgaccgtgcc gcgcctgcgg
      121 tgctggtctg ccccggcggc ggctatggcg gccatgcgat ggaccacgag ggtgagcaga
      181 tcgcgcaatg gttcaacgca aacgggctgg gggctttcgt gctgaagtac cgcctctccc
      241 cttatcgcca ccccgtgccg cttgaagacg cgaaacgcgc catgcggctc gtccgcgcga
      301 acgctgcggc ctggaatgtc gacctcgcgc gcttgggcgt catgggcttt tctgcgggcg
      361 ggcatctggc ctcgaccctg tccacccact acgagccggg caatgccacg gcggccgatg
      421 ccattgaccg gcagagctcg cgtcctgatt tttcgatcct gtgctacccg gttattacgt
      481 tcgaaccgcc gtatacgcat ttgggatctc gcaagaacct gctcggcgaa gacccacccg
      541 aggaattgat caaaaacctg tccaaccaca cccaggtgac agcccagacc cctcccgcgt
      601 tcttgtttca caccgccgac gatccggtgg tgccggttca gaacagtctc ctctattttg
      661 gcgccctgtg cgagcatgag gttcccgccg agctgcatgt ctacgaacac ggcgcgcacg
      721 gcgtgggcct ggcgcccgac ctgccggacc tcaatacgtg gcccggcctc tgcatctcgt
      781 ggttgcgcgt acggggaatt ttgcagaaat aggctgccgg ttggacgtct cggcgatggt
      841 ctcagcgtga atgttcgctg cgcagcagca gtgccaccgc atggacactg accgcttcct
      901 cacggccttc tgcgcccagg ctttcgttgg tcttggcttt gacggaaatg ttctcgacgg
      961 gaactcgcag acagctggct aacgcgttgc gcatcgcagg gatatgtgga ttcagcttcg
     1021 gcttctgggc gatgatcgtg gcatcgatat tacccaggca gtagccaagt tccttcacca
     1081 taaaatacgc ttttgcaagg agttgcaggc tgtcagcgcc tttatatcgc gggtcggtgt
     1141 ccgggaagtg ttccccgata ttgccgagcg aaacggcccc aagcagcgca tcggtgatgg
     1201 catgtgccag cgcatcggca tcggaatgtc ccgtaagtcc tttatcgtgc ggcacgtgca
     1261 ccccgccaag aatcaaggga cggcccggtt cgaggcggtg cagatcgtag ccttggccga
     1321 ttcgcattat gcaagtcctt tcgttacaac cgcttccgcg agcgccagat catccggagt
     1381 cgtgactttg aagttcagag gcgtgcctcg caccagtttc accgccgcac ccattctccg
     1441 caccagcgtc gcatcgtccg tcccggcgaa gccttgagag cgagcatacc gatgggcctg
     1501 cttaatcact tccactcgaa aggtttgtgg agtctggcag gcccagagac ggcgccgatc
     1561 gggcgtgtct tcgagcatat cgtctgcccc gcccgccagg atggtgtcag cgcaagggat
     1621 cgccacggtc gccgcaccgt attccgcggc tgcttctatc gaatccgtta tggattcatg
     1681 gcttacaaag ggccgggccg catcgtgaat aacgacgatc tcgtcgcttt gatccagctg
     1741 gtccagggcc cgttcgacgg actcctgccg ttcttttcct ccggtgacaa ggacaaaatc
     1801 attgccagca aatgagttgt gcaaaatggc ctcaaaggcc gactcagccc ccgggggaac
     1861 cgtaattaca gcgcgagaga gcagtcccgc cgcgtcaaat ctagctaacg tacgtattaa
     1921 taaaggtttg ccgccgcaca acgcgagcgc cttcggctgc tcgaggccca gccgaaggcc
     1981 catgcccgcg gcagcaacaa cgagacgtac attcacgaca agacgctttg gagcttggtg
     2041 aagatcatgc gcccggcggt ggtctggaga acgctcgtga caacgactga cacgtctttc
     2101 ccgacgtgct ctttcccacc gtcaacaacg atcatggtgc cgtcgtcaag gtagccgacc
     2161 ccctggaagg cctccttgcc ctctttgaca atacgcacct gcatttgttc atcggggagg
     2221 acggccggtt tcagggcgtt cgccaggtca ttgatattca gaacctgcac gccttcgatc
     2281 tgggcgacct tgttgagatt gaggtcgttg gtgaggattt tgctgccgta atcgcgggcc
     2341 actcgcacca gcttgctgtc aacttcggtt atctcgaccg gatcgtcctc aagtacctgg
     2401 atctcgacca gatggttgtt ttcctgcatg tctttgatga tgtcgagacc acgccgtccg
     2461 cgcgcgcggc gcaagacatc cggcgagtcg gcgatgtttt ggagttcctt aaggacgaaa
     2521 cggggaatca gcagcggccc ctcgatgaac cccgtcttgc agatatccgc gatgcgcccg
     2581 tcgataatga cgctggtatc cacgactttg gcctgcccca cgccgaactg acggcggtgc
     2641 atggcttttt tgagggattc ccagttcgat gccatggtca gcccgaggga gatgcccaca
     2701 aaaccgaaaa tgaggatcac cgacgtatac atgaataacg tgacgttggg atccgtcggt
     2761 ttggcaaagt gggtaatgta tttcacgagc ccgtacccaa ccaccatgcc gagcacgatg
     2821 cacagggcgg ccggggcgat tttttcaaag acttcctgcg tgacaaaccg caatgccagg
     2881 atcacgccca ccgagatcac cccgcccatc agcccgccga agatgcccca cggcacctcg
     2941 tcctgtaact gaatgacatc ttgtgcgagg cgcgtattct gctccatggc gtagtcgttc
     3001 cagttatcaa caagcagaac cgcccacaag gtccccatga taatacaagc tattacaaag
     3061 atgatacgaa ccgctttgat cataatctaa ctaccttcag ctctttgccg cacgatggcc
     3121 cgaggggcgc gcgcgaaaac cagccgccgc acccttcaac gacccgcagc tgccctccac
     3181 taaaaatctc tatatctccc gcttcacact gacatgcgct caatacaccc gctaaccagt
     3241 tccgtcaacc tgggctccgc gcccgacgca gtcgcaataa ttttctcgat ctgcagggct
     3301 ttcaatgcgt cgggaaaaca ctcatcggtg atcacggaaa acgccagcac gtccatacct
     3361 gcgtgtaccg ccacaatcac ctcgggcacc gtgctcatac ccactacatc ggcccccatg
     3421 ccgcgcatca tgcggtattc ggcccgtgtc tcgagacagg gccccgagca ggccaagtat
     3481 actcctttct ggagcttaat acccttttcc agggctacgg tttccgccaa actgaccagg
     3541 tcgcgggaat agggctccga catatcggga aagcgtggcc ccagcgaatc atcgttcgcc
     3601 ccaatcaacg gattgtcgcc catcaggttg atgtgatcgt cgataaccat caggtcgccg
     3661 gcgcggaaca gtggattcaa gccgcccgct gcgttaaatg caacgagaac acgcgcgccc
     3721 agcgcgcgca taacgcgcac cggaaacgtc acctggttca gggaatatcc ctcgtaacga
     3781 tggaacctgc ccgacatcgc gactacggat ttgccgctga gacgcccgaa cagcagttct
     3841 ccgtcatgcg attccacggt ggacacggca aagtgcggaa tgcggtcgta actcaagcga
     3901 gcatcaatct ttatgcgttc cacgagcttg ctcagccccg tcccgaggat tattcctatc
     3961 ccaggttcca gagcgcactc tgcccgaatg ctggagacgg cttcatcaat ccgttggcgt
     4021 aattcagaca cggcatgctc ccacgaactc agcccgggct ctttcacgtc ttagacgtgc
     4081 cccgcaggca tgcttcgact gctttccgag tgattccttc gagtacgatc cgtttctcac
     4141 gcgatttttc cccgctggcg atctcgatcg cgcgtctggg gatccccagt cttttcgata
     4201 gaaaggtttt cagagcgtcg ttggcttcgc catcaacggg tggcgctgtc aacacaatgc
     4261 ggatgcggcc atcggcttcg aaccgaactc cttccctgga agctttgggc tgaacccgca
     4321 ccgccaggta gactcttcca tccttttccg ttatggcatc cgccgccact gtcacccttc
     4381 cagccattgc cccagcgccc ggaacttctc ctgccgtttc cgtggcgacc accggtttgc
     4441 atcgcaccct tcgagaaagg cccggatatg cgtgcgcagg gtattggccg ctccatcggg
     4501 gtcgcggtgc gcccctcccg gaggttccgg caagatctcg tcaatgacgc ccaggttgcg
     4561 aagcgaatcc gcagtgatcc gaagagccga cgccgccagt tcttttcgtc cggcgtcgcg
     4621 ccagagtatc tcggcgcatc gttccggcgg acaaatcacg tagatcgacc attcaaacat
     4681 ggccacgaca tcgcccagcg cgatgcccag cgcaccgcca ctgccgcctt ccgccagcac
     4741 cacagagaag accggcgttt tcaatgccag catggcgcgg agattttccg caatcgcagg
     4801 gccctggccg cgctgttccg cctcgatgcc gggatgagcc gccggtgtgt ccacgaaggt
     4861 gatgaccgga tactgcagcc gttcggccag atggaacagg cgcaatgcct tgcgatagcc
     4921 ttccggatgg gccatgccga aattccgccg gaggcgctca tcggtcgaca cccctttttg
     4981 attgccgacc acgaccaccg tgcgcccttc gaaacggccg agcccgccca cgagcgccgg
     5041 gtcatccccc accgcccggt cgccatgcag ctcgatgaaa tcgtcaaaca cgcgcgaggt
     5101 atagtcaagc atgcggggcc gcagcggatg ccgggccacc tgcactcgct gccatggcgt
     5161 caactccgaa aacacgcgtt tcagctcggc gtccagttcg cgctggagcg ccgcgcgctc
     5221 ttcttcggtg gcggctgcac gcaaccgggt ttccagctcg acgctgtgcg cctcaaaggg
     5281 aagccacgga tccacggtgg atcgcgcatt atcaaccagt tcgatgaaac ctcgtttcat
     5341 atcctgcctc ggaagttact atctggtatt gcgtattcaa tgggcgacat tctagcacga
     5401 caacccaaga tggcgcatgc attttgtgcc gggcatctcg attgcctgta cccgtggcgt
     5461 tcctgtgaga aggaacaggc tctgcgcggc acagaacgga attgagcgaa caggtgctgc
     5521 accgtcgctg cattttcggt tcccttgatt ggtcgcggat tttttcatag ggtatgcgta
     5581 cacattcaac ttcaaaatca tggcaggcgc gtcgcagatt tcataaatgg caatgaagga
     5641 atcatccaca gatcccaagg cacggtgccc tcacttcggt tcctgtggcg gctgtgaatt
     5701 tcaggatatc gcctatccgg agcaacttgc cagaaaaagc agtgcgctct ccgcgctgtt
     5761 tgatttctgc tggccgcatc cgattccagt cgaagcgtcg cccgtcgtct ggcattaccg
     5821 caacaaagtg gatccggctt tcggcggaaa acactacgac gagccgcccc cgaaaggatt
     5881 ccagcgtgaa accgtgctcg ggttcaaacc ccggggccgc tggtttggtc ccatcgaaat
     5941 tcgggaatgc cacatcgggc cggagggcct tactccgctg ctgggcagcg tacaggcatg
     6001 gcagcgcgcc gccgggtacc ggtattacga tacgcgctca ggatccggat ttctccgcgt
     6061 actccttgtg cgcgacgcga aacgaaccgg ccagcgcatg gtcgtgctcg taaccgccga
     6121 gggcgacctg gacgccgcca gtttcgtcga ttgtgttcaa agcgcctttc catccgacag
     6181 cattcaacat gctgtctttc gcggccgggc tcagatcgcc gccgccgatg aattgcgtgt
     6241 tctttacggc gcctccgata tcaccgaaat tctccacgtg tccgatggcg atgccgtacc
     6301 tgcccgcaga ctgtcgctga gggtctcgcc gttcagtttt ctccagacca acacgctcgc
     6361 cgcagagagg ctctacgcga tgattcgcgc gtggacgaaa caggccgcgc catccacatt
     6421 gcacgatctc tacggcggta tggggtcgat cgtcttcgcc tgtgcggacc tcgccggcga
     6481 agtgcactcc gtggagaacg tccccga
//
LOCUS       DAOPBQ010000167         6501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10497_length_6501_cov_3.674061, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000167 DAOPBQ010000000
VERSION     DAOPBQ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10497_length_6501_cov_3.674061"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /gene="trpC"
                     /locus_tag="PKW60_06850"
     CDS             <1..687
                     /gene="trpC"
                     /locus_tag="PKW60_06850"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPJ99188.1"
                     /translation="LGAPRDFRAALRGKGISLIAEVKKASPSKGVLRADFNPAELAGQ
                     YERAGADAISVLTDEKYFQGALADLEAVRRQVAVPCLRKDFIVDEYQLYEARAAQADA
                     VLLIVRALSDDQLRDYLNVAKSLQMAALVETHDVRDIERAINAGAHILGINNRDLATF
                     QVSLNTTLELRRHVPGGHVLVSESGIHTREHVQLLEDGGIDAILVGESLVTSDNIGAK
                     IRELLPRDQG"
     gene            674..1303
                     /locus_tag="PKW60_06855"
     CDS             674..1303
                     /locus_tag="PKW60_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPJ99189.1"
                     /translation="MTRVKICGITNLDDALAACDAGADALGFVFAEEARRRNRHIAPE
                     EARAIIAQLPPFVTTVAVCVNAPLAELRSFLAFTDLVQLCGEEDPEMYRPVADRALKV
                     IHVGQESKLGDWRNHPARAIVLDTLAPGTHGGSGVPFDWEMARAATELGRPIMLAGGL
                     TPENVAEAVKTVQPYAVDTSGGVEAAPGKKDHDRIRQFIRNARSNLSVS"
     gene            1248..2489
                     /gene="trpB"
                     /locus_tag="PKW60_06860"
     CDS             1248..2489
                     /gene="trpB"
                     /locus_tag="PKW60_06860"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPJ99190.1"
                     /translation="MTESANLSAMPAQTYPYPDARGRYGPFGGKYVPETLMYALEELE
                     DAFRAAFQDQAFLQALEGYRRDYIGRPTPLYFAKRFTGHLGGAKVYFKREDLAHTGAH
                     KINNALGQVLLAQRMGKTRIIAETGAGQHGVATATAAALLGLECEIYMGEEDIERQKL
                     NVFRMRLLGSKVNPVASGSKTLKDAINEALRDWVTNVRHTHYVLGTVHGPHPFPLVCR
                     EFQKVIGQEARRQILEAEGRLPDVLLACVGGGSNALGLFFDFISEPGIRMIGVEAGGL
                     GVETGKHAARFAGGKLGTLQGAMSYVLQDDNGQILGTHSVSAGLDYASVGPEHAFLRS
                     EGRVEYVNVTDDEAITAFKLTSELEGIIPALESAHALAHAQKLVLQLPADQIVIINMS
                     GRGDKDVQQAARYLLPGEALL"
     gene            2486..3286
                     /gene="trpA"
                     /locus_tag="PKW60_06865"
     CDS             2486..3286
                     /gene="trpA"
                     /locus_tag="PKW60_06865"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPJ99191.1"
                     /translation="MNRIEARFDELRRQGRKAFIPYITAGDPTLAGTAEIVLALEQAG
                     ADVVELGIPFSDPLADGAVNQEAALRALRHGTSLRDVLQMVKALRQRSQVPIVLFTYF
                     NPVHSYGLDQFAPDCRDAGVDGVLCVDLPPEEAGEYKASMDALDIATVFLLAPTSTEE
                     RIEIVARHSTGFIYYVSRTGVTGEQARLSEDVRSMVERIKQHTDKPVAVGFGISKPEH
                     AAEIARYADGVIVGSALVRLIGQLGDSPEMPSGVAAFAKTLADAAKSL"
     gene            3319..4635
                     /locus_tag="PKW60_06870"
     CDS             3319..4635
                     /locus_tag="PKW60_06870"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPJ99192.1"
                     /translation="MADAVEAALRIGAVEAEALETTSRSFEVEVSRGEIETLASSSAR
                     GLGLRLFTSDKRMGFAYTASSDEPLETTVRNAWNNAISNDPEPYGNICRDAGVFDADW
                     SEQAPADVPVEAKISFAKDLEKATLAADSRIVMVERAAYGDSLVDFALVNSAGVCRRY
                     QNAYFSCSAGAVAAREGVDSESGWEFDVARTFDGLRLDWVAQRCAQDATRKLGGKPCA
                     TGAMPVVLDNAVATRFLAMFGSALRGDNVLKGKSLFAGSVGEAIASDCLTFVDQNDCP
                     DAVGRAPFDAEGTLAKRTVLVENGRLQGYLHNQQTASEMGEKPTANAVRGFRSPPAVG
                     PSSLYIVPGGVSQEGLFGKAPRGLFVTQALGMHSANLITGDFSFGASGLLFESGELRA
                     PVRGVTIAGNFRPLLKAVEAVGNDLRFLGSCGAPSLLISELVVSGE"
     gene            4636..5538
                     /locus_tag="PKW60_06875"
     CDS             4636..5538
                     /locus_tag="PKW60_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPJ99193.1"
                     /translation="MDGNLQALNALLAVFSFVLGSMIGSFLNVCVYRLPRGLSVVKPR
                     SKCPGCDNPIAWYDNIPIVSWLVLGAKCRQCGQPISWQYPLVEAATGALFLLVFWRFG
                     LVLATPIYMALAAALVLVTFVDLTDWTIPDEVTLPGIPAGIAASVLFMLYPESGLSVM
                     GPFEMPVFNSLLGVLVGGGILYALDKGALLLLKKPGMGFGDVKLNAMLGAFFGLYGAI
                     LIIIIAAFIGSFVGILVVLAGKRKSAQDGQPQSADTENAGPPAPGHYLPFGPYLALAG
                     VVVMLAGPAIIHFYFNTLLASPSL"
     gene            5566..>6501
                     /gene="aroC"
                     /locus_tag="PKW60_06880"
     CDS             5566..>6501
                     /gene="aroC"
                     /locus_tag="PKW60_06880"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPJ99194.1"
                     /translation="MLRYYSAGESHGRGIFAFLEGFPAGLQVQPDDINFWLAERQKGY
                     GRGGRQRIEKDEVDVLSGIRGGVTLGSPILLAIWNRDFKNWESAMDPWKPSTGERAVK
                     VVRPRPSHADLVGALKYEHDDCRNILERASARETAARVAAGALCRKLLAEFGVDATGH
                     VVQIGEAVADTDSIPTEELRERSMASDVRCADPEASQHMHDAIQQAKRDKDTLGGVIE
                     VRAWGLPAGLGSHTQWYRKLDAAIAQALMSIQAVKGVEVGIGFRGAHLRGRKYHDEIV
                     WSDVPQGGSHYVRSSNHLGGTEGGMSSAGELVVRVV"
BASE COUNT         1239 a         1953 c         2038 g         1271 t
ORIGIN      
        1 ctgggcgcac cgcgtgattt ccgtgctgca ctccggggaa agggcatcag ccttatcgcg
       61 gaggtcaaga aagcctcgcc cagcaagggc gtgttgcggg ccgacttcaa cccggcggag
      121 ctcgcgggac agtacgagcg tgccggcgca gacgcgatct cggtcctcac ggatgagaag
      181 tactttcaag gcgccctggc cgacctggaa gcggtgcgcc gccaggtggc tgtgccgtgc
      241 ctgcgcaagg atttcatcgt agatgaatac caattgtacg aagcccgtgc cgcgcaggcg
      301 gatgcggtgc tgctgatcgt ccgcgctttg tctgatgacc aattgcgcga ttatctgaat
      361 gtggcgaaaa gcctccagat ggctgcgctt gttgaaacgc atgatgtccg cgacatagaa
      421 cgcgcgatca acgccggtgc gcatatcctg gggatcaaca acagggatct ggccacattt
      481 caggtctcct tgaacaccac gttggaattg aggcgccatg tgcccggggg gcatgtgctg
      541 gtcagcgaaa gcggcattca cacccgggag cacgtacaac tgctggaaga tggaggaatc
      601 gacgccattc tggtcggcga atcccttgtg accagtgaca acattggggc gaagatccgg
      661 gagcttctgc cacgtgacca gggttaaaat ctgcggtatc acgaatcttg acgacgccct
      721 cgcggcgtgt gacgcgggtg ccgacgcgct cggtttcgtg ttcgccgaag aggcccgccg
      781 gcgaaaccgc cacatcgcac cggaggaggc ccgggcgatc attgcgcagc tgccgccgtt
      841 tgtgacgacc gtggcggtct gtgtgaatgc cccgctggcc gaactgcgtt cgtttctcgc
      901 gttcacagat ttggttcaat tgtgcggcga agaagatccc gaaatgtatc gtcccgtggc
      961 ggatcgcgct ctgaaggtga ttcacgtcgg tcaggagtcc aagctcggcg attggaggaa
     1021 tcatcccgcg cgtgccattg tattggatac gttggcgcct ggaacccatg gcggctctgg
     1081 cgtccctttc gattgggaaa tggcccgtgc cgcaacggaa cttggcagac ccattatgct
     1141 ggctggcggc ctgacgccgg agaacgtggc tgaagccgtt aaaaccgttc aaccgtatgc
     1201 cgtggatacc tcgggcggcg tggaagccgc acccggaaag aaagatcatg accgaatccg
     1261 ccaatttatc cgcaatgccc gctcaaacct atccgtatcc tgacgccaga gggcgctacg
     1321 gcccgtttgg cgggaagtac gtgcctgaaa cgctgatgta tgcgctggag gagctggaag
     1381 acgcttttcg cgcggctttt caggatcagg cctttctgca ggcgcttgaa gggtaccggc
     1441 gcgactacat cggccggccc acgcccctgt attttgcaaa acggtttacc gggcatctgg
     1501 gcggggcgaa agtctatttc aaacgcgagg acctggcaca taccggcgcg cacaagatca
     1561 acaacgccct cggccaggtg cttctcgccc agcgaatggg gaaaacgcgc atcatcgccg
     1621 aaaccggcgc cggccagcat ggtgtcgcta cggcaacagc cgccgcgctc ctcggcctgg
     1681 aatgcgaaat ctacatgggc gaggaagaca ttgagcgcca gaaactgaac gtcttccgca
     1741 tgcgcctcct cggctccaaa gtgaatccag tcgcttccgg ctcaaaaacc ctcaaagatg
     1801 cgatcaacga ggcgttgcgc gactgggtca ccaacgttcg ccacacgcac tatgttctgg
     1861 gcacggttca cgggccgcac ccgtttcctc tggtctgccg ggagtttcag aaggtcatcg
     1921 gccaggaggc ccggcggcag attctggagg cggaaggccg ccttcccgac gtgttgctgg
     1981 cctgtgtagg aggaggcagc aatgcgctcg gcctcttctt cgacttcatc tcggagcccg
     2041 gtatccgcat gatcggggtg gaagccggcg gcctgggcgt cgaaaccggt aaacatgcgg
     2101 cccgatttgc aggcggcaaa ctgggcacct tgcagggggc gatgagctat gtgctccagg
     2161 acgataacgg acagatcctg ggcactcaca gcgtttcggc cgggctggac tatgccagtg
     2221 tcgggcccga gcatgcgttt ctacgcagtg agggccgtgt cgagtacgtg aacgtcaccg
     2281 acgatgaagc gataaccgca tttaagctga cgagcgagct cgaaggcatc atcccggcac
     2341 tcgaaagcgc ccatgctctc gctcatgcac agaaactcgt cctacaactc cccgcagacc
     2401 agatcgtgat catcaacatg tccggccggg gagacaagga cgtacagcag gcagcccgat
     2461 atctgctgcc gggggaagcg ttgttgtgaa ccgaatcgaa gcacgttttg atgaattgcg
     2521 gcgccaaggc cgcaaagcct ttatcccgta catcacggcg ggcgacccca ctctggccgg
     2581 caccgccgag atcgtcctgg cgctggagca ggccggcgcc gatgtggtcg agctcgggat
     2641 tccattctcc gacccgctcg cggatggcgc cgtgaaccag gaagccgctt tgcgtgccct
     2701 gcgccacggc acctcgctcc gggatgtcct ccagatggtg aaggcactgc ggcaacgcag
     2761 ccaggtgccc atcgttctgt tcacgtattt caatcccgtg cattcctacg gcctcgatca
     2821 attcgctccg gattgccgtg acgcgggagt ggatggcgtg ctctgcgtcg acctgcctcc
     2881 cgaagaagcg ggggagtata aggcgtccat ggacgcgctg gatatagcca ccgttttcct
     2941 cctggctcca acaagtacgg aggagcgaat cgagatagtc gcgcggcaca gcaccggttt
     3001 catctattac gtatcccgga cgggcgtcac cggtgaacag gcacggctca gcgaagacgt
     3061 gcgcagcatg gtcgaaagaa ttaaacagca cacggacaag cctgtcgccg tcggcttcgg
     3121 catatcaaaa cctgaacatg ccgccgagat tgcccggtat gccgacggcg tgatcgtggg
     3181 cagcgccctg gtccgtctga taggacaatt gggcgattca cccgaaatgc cttctggcgt
     3241 ggcggcgttc gcaaaaacgc tcgccgacgc ggcaaaaagt ctctaaagac cgggggtttc
     3301 tcagtggaaa accagcgatt ggccgacgcc gttgaggccg ctctgcgcat cggggcagtc
     3361 gaagccgaag ccctcgaaac caccagtcgg agctttgaag tcgaagtatc ccggggtgag
     3421 atcgaaaccc tggcctcctc ctccgcgcgt ggtctcggtc tccggctgtt tacctccgac
     3481 aagcgcatgg ggtttgccta cacggcctct tccgacgaac cactcgaaac cacggtccgt
     3541 aatgcgtgga acaatgccat cagcaacgac ccggaaccgt atggaaatat ttgccgtgac
     3601 gccggcgtct tcgatgcgga ctggtcagag caggcgcctg cagatgtccc ggtcgaggct
     3661 aaaatatctt tcgctaaaga cctggaaaag gccacgctgg ctgccgactc ccgcattgtg
     3721 atggtcgagc gggcggcgta cggcgacagc ctggttgatt ttgcgctggt gaactctgcg
     3781 ggggtatgcc gccggtatca aaacgcgtat ttcagttgct ccgcgggagc tgtggccgcc
     3841 cgggaaggag tcgattcgga aagcggttgg gaatttgacg tcgcccgcac ctttgacggg
     3901 ctccgcctgg attgggtcgc acaacgttgc gcccaggacg ccacccgcaa gctcggcggc
     3961 aaaccctgcg ccaccggcgc catgcctgtg gtgcttgata acgccgtggc tacccgcttc
     4021 ctcgccatgt tcgggtcggc gctccgtgga gacaacgtcc tcaagggcaa atctctgttt
     4081 gcgggatctg tcggcgaggc cattgcatca gattgcctca cgttcgtcga ccagaacgat
     4141 tgcccggatg cggtcggccg tgcgccattt gacgccgaag gcacgctcgc caagcgcacg
     4201 gtgctggtgg agaacggccg gctgcaggga tatctccaca accagcagac ggcatccgaa
     4261 atgggggaga aacccaccgc taacgctgta cgaggcttca gaagcccgcc cgccgtgggt
     4321 ccgtccagcc tctacatcgt ccccggcggc gtctctcagg aaggtttatt tggcaaggcg
     4381 ccccgcggcc tgttcgtgac ccaggccctg gggatgcatt ccgcgaacct gatcacgggc
     4441 gatttcagct tcggcgcctc aggcctgctg tttgaatcgg gcgagttgcg cgctcccgtg
     4501 cgtggcgtga ccatcgcggg caactttcgc ccgctcttga aagcggtaga ggcggtgggc
     4561 aatgatctgc gttttctggg gtcgtgtggc gccccgtcgc tgttaatctc ggaactggta
     4621 gtgagtgggg aataaatgga cggcaatctg caggcattga atgcgcttct ggcggtcttt
     4681 tcgtttgtac ttggaagcat gattggaagc tttctcaacg tatgcgtgta ccgcttgccg
     4741 cgtggcctgt cggtcgtcaa acctcgctcg aagtgtccgg gatgcgacaa tcccatcgcg
     4801 tggtacgaca acatccccat cgtcagctgg ctggtgctgg gcgccaaatg ccgccagtgc
     4861 ggccagccca tcagttggca gtatcccctg gtggaggccg cgaccggcgc cttgtttctg
     4921 ctggtcttct ggcgtttcgg cctggtgctc gccacgccca tctatatggc gctcgcagcc
     4981 gcgctcgtgc tcgtcacctt tgtcgacctg accgactgga ccatccccga tgaggtgacc
     5041 ctgcccggca ttcccgcggg tatcgcggcg tcggttctgt ttatgctcta cccggagagc
     5101 ggcctgtccg tcatgggacc gttcgagatg cctgtgttca actccctgct cggggttctt
     5161 gtgggcggcg gtattctgta cgccctggac aagggcgccc tgctgctcct caaaaagccg
     5221 gggatggggt tcggtgatgt caagctcaat gccatgctcg gagcattttt cgggctgtat
     5281 ggcgcgattc tgatcatcat catcgccgcg ttcatcggga gttttgtcgg catcctcgtt
     5341 gttctggctg gaaaacggaa atctgcacaa gacggccagc cccaaagcgc agacacagag
     5401 aatgccggtc cgcccgcgcc agggcactac ctcccgttcg gaccctatct ggcgcttgca
     5461 ggcgttgtgg tcatgctggc cgggccggcg atcattcatt tttacttcaa cacgctgctt
     5521 gcgtccccgt cgctgtgacg caggcccaaa ctgaggatac gtatcgtgct tcgctactat
     5581 tcggcaggtg aatctcacgg gcgcggcatc tttgcgtttc ttgaaggttt ccccgcgggc
     5641 cttcaggttc aaccggacga catcaacttc tggctggcgg agcgccagaa gggatacggc
     5701 cggggcggac gccagcgtat cgagaaagat gaagtggacg tactctccgg cattcgcggc
     5761 ggcgtcaccc tgggcagccc gatcctgctc gcgatctgga accgcgattt caagaactgg
     5821 gaaagcgcga tggatccctg gaagccatcc accggcgagc gtgccgtgaa agtcgtgcgg
     5881 ccacgtccca gccatgccga tctcgtgggg gcgctcaaat acgaacacga cgactgccgc
     5941 aacatcctcg aacgggccag cgcgcgcgaa accgcggcgc gtgttgccgc cggggccctc
     6001 tgccgcaagc tgcttgcgga attcggcgta gatgcaaccg ggcacgtcgt ccaaatcggt
     6061 gaggccgtag ccgacacaga ctctatcccc acggaggagc tgcgggaacg ttccatggcg
     6121 tctgatgtgc gctgtgccga ccctgaagcc agccaacata tgcatgatgc aattcaacag
     6181 gccaaacgcg acaaagacac cctgggcggc gttatcgaag tgcgcgcgtg gggactcccc
     6241 gccggtctcg gcagccacac ccagtggtac cgtaagctcg atgccgccat cgctcaggct
     6301 ctgatgagca ttcaggcggt caaaggagtt gaggtgggca tcggtttcag aggggcccat
     6361 ctgcgcggac ggaaatacca cgacgagatt gtctggtcgg acgtccctca aggcggcagc
     6421 cattacgtgc gcagctcgaa ccatctgggt ggaacggaag gggggatgtc atccgccggt
     6481 gaactggtgg tgcgcgtggt c
//
LOCUS       DAOPBQ010000168         6498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10504_length_6498_cov_3.708055, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000168 DAOPBQ010000000
VERSION     DAOPBQ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10504_length_6498_cov_3.708055"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..1886
                     /locus_tag="PKW60_06885"
     CDS             438..1886
                     /locus_tag="PKW60_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99195.1"
                     /translation="MRHHGAWIVCCMFGFLAAIAVPPAQGAVLQVGASTVSITPDQPV
                     ALSGQMSTRIAREVQSVLLANALALESQNEGAALEQVIFVACDVVAIPEHIRTRAIEL
                     LAPRVPDFDTNKLIISATHTHTAPVMIEGVYVIPDEDVMRPAEFSEFFSARIADVAEA
                     AWKNRKPARAGWGMGDAVVAYNRRSFFEDGHAQMYGNISIDEFRGIEGPEDHAVEVLF
                     FWDEDDSLIATAVNIACTAQEVEGLSVVNADFWHPVREGLKAKHGEGLVVLGWIGAAG
                     DQSPHIRFRQAAEDRMRELRGLSRLDEIARRIIRAWEEAYEGGQKEKFSDVPLVHTVK
                     SIELPQRLVTEQEYKDTQAEIAAASTDPRDYRRVEWLQNVLKRYEQQQQGDIGPYTMD
                     LHVLRLGDVAIAVNPFELFTQYGIQMKAKSKALHTFVIELAGPGTYVPTPFAAAGGGY
                     SAIAASNQVGPEGGQKLVDETVAAVNALWSEQ"
     gene            complement(1945..4758)
                     /locus_tag="PKW60_06890"
     CDS             complement(1945..4758)
                     /locus_tag="PKW60_06890"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPJ99196.1"
                     /translation="MNSVHGGQNSAPVYCQTIDEVLETLSTDARRGLSQSEASARLEK
                     YGPNELLSEEPVPAWKRFLAQFKDVLVILLLIATAISVLLWLVERQSALPYEGIVIFA
                     IVLLNAFLGFIQEARAESAVTALRRMVSEMASVLRDGERRRIPTAELVPGDIILIEEG
                     DEIPADARLIHCTALQTAEAALTGESLPVSKETAPMDSEAPLGDRVNMLFSGTAATYG
                     RGSAVVVATGMQTELGRIAGMLRQAPRELTPLQVELDRVGKLLGIIVVIIAIVMIATL
                     IFVENLRGLAALFEALILGVALAVAAVPEGLPAVVTTVLALGVQRMARRNAIVRHLAA
                     VETLGSATIIASDKTGTLTKNEMTVRTVVTASGQVELEGTGYEPEGRVHVNGNAVSDE
                     ALRTEVEHALMAADRANNAVLQHKNGRWTVLGDPTEGALLVAARKTGLSGESLDVRFE
                     RVGEVPFSSERKLMSTVHSDAEAPERLLVFTKGAPDVLLSRCTLEQTGGATRPLTEQR
                     RREINASNNELARQALRTLGVAAREIPTDTLVQEQPDERLERDLVFLGLIGMIDPPRA
                     EAKPAVERAQRAGIRPMLITGDHPATAEVIAEELGIAPRGRTVTGAELAKMPEDALTE
                     TVQEVFVYARVDPEHKLDIVRALQESGATVAMTGDGVNDAPALKAADIGIAMGITGTD
                     VSREAADVVLTDDNFATIVAAVEEGRAIFDNIRKFLRYLLSSNIGEVMTMFFGVLLAK
                     SMGLMAQGHGVVMPLLATQILWVNLVTDGAPALALGVDPPAPDVMLRKPRPRQEGVIT
                     PGMWVTIALTGVVIAAGTLFVLDASLPGGLVEGQGDMRYAQTMAFTTLVVFQMFNVFN
                     ARSDEHSAFQGFFQNNWLWGALILSMGLQVAVVYVPFLQDAFSTCALSLKDWGVCTAV
                     ASSVVWVRELSKIVARMAKRTRA"
     gene            complement(5002..>6498)
                     /locus_tag="PKW60_06895"
     CDS             complement(5002..>6498)
                     /locus_tag="PKW60_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99197.1"
                     /translation="LGAKLQACNSFEASTVPYIPVPGSLYQKYAAMRRLGVTSVMQCW
                     YMGNMPSVMHRAAASVLPFAPDDLDEETFLLELARRDWGPVHAARVVRAWRLFGEAYD
                     NYPISNAFQYYGPINDGIVWPLHLKPAHKNLSPVWKLEFPPSGDRIGECFSGNHTMEE
                     VLELCGRMSAIWRIGVDELLELAPFCAGDPERQRDLTVAQALGIQFQTGYNILRFYDM
                     REKLLYDASDSRAELLAAMRDIVEEEIENATAMAALCEINPFLGFQAEAEGYTYFPAE
                     LRWRVERLQEVLETEFVEAENAIAAEKPVFPEESGLLDGPLTYRCQRAEEDFASHWRE
                     ASAWTGLPQTALPVEGLAWAWQAAHDDAFLFVNVECPSSEQWRPVAVAVAVEPTHIYP
                     RRTFRGDVNGKQVARLGWLVPDTPWDLVVDSLEGRQAFRLRIPMEAFRGEMAPSTPMR
                     INVEVAGRSSDNTGTMLASWAPTPEVTVMPRLGYGADNPARMGWLRLE"
BASE COUNT         1256 a         2064 c         1952 g         1226 t
ORIGIN      
        1 acaagcctgc gattccatcg gccaccccga gcaggtactt gccagcgcaa cgattccccg
       61 cgaggaagtc cgaaacactc cgcgtatact tgctggtcac atagcccgac cagcaaatga
      121 acgcaaccat tgcccccaag accatccagt ccagcgcttg catattcata gtgagcctga
      181 taccccctga cgcgattcag tcagaccatg tcatattttg gcgatgcacc agggcattct
      241 accagatgaa gcgcccagca ggaactgtct tcacgatgaa aatcatggta agcgaagcga
      301 tgtgccttct gtctccaccg cccaggtgcg cgcaaacccg gcgctgcgca ccggatgatt
      361 tatgattttg cgctgattgg ggcagatcag atagcctatc cgggcgtgga atccgcagac
      421 agaagggaga ggccggtatg agacatcatg gtgcctggat cgtatgctgc atgtttgggt
      481 ttctggctgc tattgccgtg cctcctgcgc agggtgcggt tttgcaggtg ggcgcatcaa
      541 ccgtaagcat tacgcctgac cagcctgtag ctctgagcgg ccagatgtcg acgcgtatcg
      601 cgcgtgaggt gcagagcgtg cttctggcca atgcactcgc gttggaatca caaaatgagg
      661 gagcggctct cgaacaggtg attttcgtgg cctgcgacgt cgtggccata cccgagcaca
      721 tccgaacgcg cgcaatcgaa ctcctggcgc cgcgtgttcc ggatttcgac acgaacaaac
      781 tcatcatcag cgcgacccac acgcataccg cgcccgtaat gattgaaggc gtttatgtca
      841 tcccggacga ggatgttatg cggcccgcgg agttttccga attcttctcg gcacgcattg
      901 ctgatgtcgc tgaagcggcc tggaagaacc gcaaacccgc ccgggcggga tggggcatgg
      961 gggatgccgt tgtcgcatac aaccgccggt catttttcga agacggccat gcccagatgt
     1021 acggcaacat cagcatcgat gaattccggg gtatcgaagg cccggaggac catgccgtgg
     1081 aagtgctctt cttctgggat gaggacgaca gccttatcgc gaccgccgtg aatatcgcct
     1141 gcacggcgca ggaagtggaa ggcctcagtg tggtcaatgc cgatttctgg cacccggtcc
     1201 gcgagggcct gaaagcgaag catggcgagg gactggtggt tctgggctgg atcggcgcgg
     1261 caggcgacca atcgccccac atccggttcc gccaggccgc cgaagaccgc atgcgcgagc
     1321 tgcgcggcct gagccggctc gacgaaattg cccggcgcat catccgcgcc tgggaggaag
     1381 catatgaggg cggacagaag gagaagtttt cggatgtgcc tctggtgcat accgtgaaat
     1441 cgatcgaatt gccccagcgc ctggttacgg agcaggagta caaggacacg caggccgaga
     1501 tcgctgccgc ctccactgat cccagggatt accggcgcgt ggaatggctc cagaatgtgc
     1561 tgaaacgcta tgagcaacag cagcaaggcg acattgggcc ctacaccatg gacctgcatg
     1621 tccttcgcct tggcgatgtg gccattgccg tgaatccttt cgagctcttc actcaatacg
     1681 gcatccagat gaaagcgaag agcaaggcct tgcacacatt cgtcattgag ctcgccgggc
     1741 cgggcaccta tgtgcccacc ccatttgcgg ctgccggtgg aggctacagc gccatcgcgg
     1801 cgagcaacca ggtgggtccg gaaggtggtc agaagctggt cgacgaaacg gtcgccgcgg
     1861 tcaacgctct ctggtcggaa caatagctgc cgggggaatt tgccaaagcc ggcgcggatt
     1921 gccggttcga tgcagttttc gtgatcaggc gcgcgtgcgt tttgccatgc gcgccacgat
     1981 cttgctcagt tctcgaaccc agaccacgct gctggcaacg gcggtgcaca cgccccaatc
     2041 cttcaggctc agggcgcagg tggagaaggc gtcctgcaga aacgggacgt aaaccacggc
     2101 cacctgcagg cccatcgaga gaatcagcgc gccccacagc cagttgttct ggaaaaatcc
     2161 ttgaaacgcg ctgtgctcgt cggaacgcgc gttgaacacg ttaaacatct gaaatacgac
     2221 cagtgtggtg aacgccatgg tttgcgcata ccgcatgtcg ccctgacctt ccaccagacc
     2281 gcccggcaag gacgcatcga gcacaaacag tgtcccggcg gcgatcacta cgcccgtgag
     2341 cgcaatcgtg acccacatcc ccggcgtgat cacgccttcc tggcgaggcc gcggtttgcg
     2401 gagcatgacg tccggagccg gcggatcaac gcccagtgcc agcgcgggcg cgccgtccgt
     2461 caccaggttg acccagagga tctgcgtggc cagcagaggc atcaccacgc cgtgcccctg
     2521 cgccatcaac cccatggatt tcgccaggag cactccgaaa aacatcgtca tgacttcacc
     2581 gatgttcgac gagagcaggt accgcaggaa tttgcggatg ttgtcgaaga tggcccggcc
     2641 ttcttcaacc gccgcgacga tggtcgcgaa attatcgtcc gtcagcacga cgtcggctgc
     2701 ttcacgggag acgtcggtgc cggtaatgcc catggcgatc ccaatgtccg cggctttgag
     2761 cgccggggcg tcgttcacgc cgtcgccggt catggctacc gtcgcgccgc tctcctgcag
     2821 cgctctcacg atatccagct tgtgctccgg gtccactcgc gcatagacaa atacctcctg
     2881 gaccgtttcc gtcagagcat cctccggcat cttcgccagt tccgcgccgg tgaccgtccg
     2941 gcctctgggc gcaatgccaa gctcttcggc gatgacctcg gcggtcgcgg ggtgatcgcc
     3001 cgtgatcagc atcggacgga tacccgcgcg ttgcgcacgc tccacagctg gcttcgcctc
     3061 ggcgcgtggc ggatcgatca tcccaatcag gcccagaaaa acgaggtcgc gttccagcct
     3121 ttcgtcgggt tgttcctgca cgagcgtatc cgtcgggatc tctcgcgcgg caacgcccag
     3181 cgtgcgcagg gcctgccggg ccagctcgtt attcgaggcg ttgatctccc ggcggcgctg
     3241 ttcggtcaag ggcctggtgg cgccgcccgt ctgttccaga gtgcagcggg acaacaggac
     3301 gtccggcgct cctttcgtga acaccagcag gcgttcggga gcttcggcgt cgctatgaac
     3361 ggtcgacatc aatttgcgct cggatgagaa ggggacttcc ccgacgcgct caaaccgcac
     3421 atccagcgat tcgccagaca gaccggtttt ccgtgccgcc acgagcagcg cgccttccgt
     3481 cgggtcgccc agcactgtcc agcggccgtt tttgtgctga agcacggcgt tgttggcgcg
     3541 atcggcggcc atcagggcat gctccacctc tgtgcggagc gcctcatccg aaactgcgtt
     3601 gccgttcacg tgtacccggc cttcgggctc gtatcccgtg ccttcaagct ccacctggcc
     3661 gctcgcggtg actacggtcc gcaccgtcat ttcatttttg gtcagggtgc cggtcttgtc
     3721 ggaggcaatg atggtggcgg agccgagcgt ttcaacagcg gccagatggc gcacaatggc
     3781 gttgcgcctg gccatgcgtt gcaccccgag tgcgagcacg gtggtcacca ccgcgggaag
     3841 gccttccggc accgccgcaa ccgccagggc cacacccagg atgagcgctt caaacagcgc
     3901 cgccaggcca cggaggttct cgacaaaaat caaggtggcg atcatcacga tggcgattat
     3961 gacgacaatg atcccgagta atttgcccac ccggtccagt tcgacctgaa gcggcgtgag
     4021 ttcccgggga gcctgccgca acatgccggc aatccgcccg agttcagtct gcatgccggt
     4081 ggccaccacc actgcgctgc cccgcccgta ggttgcggcc gtcccgctga aaagcatatt
     4141 gacgcggtcg cccaggggcg cctcgctatc catgggggcc gtttctttag agaccggcag
     4201 gctctcaccg gtcagcgccg cctcggccgt ctgcaacgcc gtgcagtgaa tcagccgtgc
     4261 gtcggccgga atctcatcgc cttcctcaat cagaatgatg tcgccgggca ccagttccgc
     4321 tgtggggatc cgccgccgct cgccatcacg caggacggag gccatttccg agaccatacg
     4381 ccgcaacgcg gtgacggccg attcggcccg ggcttcttga atgaagccga ggaacgcgtt
     4441 cagcagcacg atcgcgaaga tgacaatgcc ttcgtatgga agcgccgatt gccgttccac
     4501 aagccaaagc aacaccgaga tcgcggtcgc gatcagcagc aaaatgacca gaacgtcctt
     4561 gaattgcgcg agaaaccgtt tccacgccgg cacgggctct tccgaaagca gctcgttggg
     4621 tccgtatttt tcgagcctcg cgctggcctc ggactggctt aatccccgcc gtgcatccgt
     4681 gctcaaagtc tcaaggactt catcaatcgt ctggcagtat acgggcgcag agttctggcc
     4741 accgtgtacg ctattcacag tgcaatcctt ccaggctcgc gggcccggct gcgccgtgct
     4801 ggagaaaggc aggccggcgc acgagccgtt accgcctcaa aagcgaaaac ggtccccgtg
     4861 cctgacctga ggtttccgtt tcgatcggtg gccgccggga cacaggctgc ggttattgtg
     4921 gacgctctat tctatcacga agaacgccct gggcgtctca ttctgtgcag cgcgttccgc
     4981 gtgtccagcc ccgggcgtga gctactccag ccgtagccac cccattcggg ccgggttgtc
     5041 ggcgccatac cccaggcgcg gcatcacagt cacttcgggg gtaggcgccc agctggcgag
     5101 catcgtgccg gtgttatcgc ttgagcgccc ggcaacctcc acgttaatcc gcatgggcgt
     5161 ggacggcgcc atttcgccac ggaatgcctc cataggaatg cgaagccgga atgcctggcg
     5221 gccttcgagg ctgtccacga caaggtccca cggcgtgtcg ggaaccagcc agcccagccg
     5281 ggcgacctgc ttcccgttca cgtcaccgcg gaaggtgcgg cgcgggtaaa tgtgcgttgg
     5341 ttctaccgcc accgcgacgg cgaccggacg ccactgttct gacgaggggc attccacgtt
     5401 cacaaagaga aaagcgtcat cgtgcgcggc ctgccacgcc cacgccagcc cttcaacggg
     5461 caaggccgtt tgaggcagcc ctgtccaggc ggacgcttcc cgccagtgtg acgcaaaatc
     5521 ctcttccgcc cgttggcagc ggtacgtcag cgggccgtcc aggagcccgg attcttcggg
     5581 aaacacgggt ttttcggcag cgatggcatt ttcagcctcc acaaattcgg tttccagcac
     5641 ttcctggagc cgctccaccc gccagcgcag ttcggcaggg aaataggtgt agccctcggc
     5701 ttcggcctga aatccaagga aagggttgat ctcacacagc gccgccatgg cggtagcgtt
     5761 ttcgatctcc tcctcgacga tgtcccgcat tgccgcgagc aactcggccc ttgaatcact
     5821 tgcatcgtag agaagctttt cgcgcatgtc gtaaaagcgg aggatgttgt atccggtctg
     5881 gaactgaatg cccaaggctt gggctacggt gagatcgcgc tgccgttccg ggtctccggc
     5941 gcagaagggc gcaagctcca gaagctcatc cactccgatc cgccagatgg ccgacatccg
     6001 cccgcagagt tcgagcactt cttccatcgt atgattgccg gagaaacact ccccgatccg
     6061 gtcgccgctc ggaggaaatt ccagcttcca gaccggcgag aggtttttgt gggcgggttt
     6121 caaatggagc ggccacacaa tgccgtcgtt gatggggccg taatactgga atgcattgga
     6181 gatcggataa ttgtcgtatg cctctccaaa cagccgccac gcccgtacaa cgcgagccgc
     6241 atgaactgga ccccagtccc gccgcgccag ctccaggaga aacgtttcct cgtcgaggtc
     6301 atctggtgca aatggtaata cgctcgcagc cgcccggtgc atgaccgacg gcatattgcc
     6361 catataccag cactgcatga cgctcgtgac gcccagccgg cgcatcgcag catatttttg
     6421 atagagactg cccgggaccg gaatataagg gaccgtcgac gcctcaaagg agttgcaggc
     6481 ctggagcttt gcgcccag
//
LOCUS       DAOPBQ010000169         6479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10554_length_6479_cov_3.405978, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000169 DAOPBQ010000000
VERSION     DAOPBQ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10554_length_6479_cov_3.405978"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>6479)
                     /locus_tag="PKW60_06900"
     CDS             complement(<1..>6479)
                     /locus_tag="PKW60_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPJ99198.1"
                     /translation="EAAKANWRITPAGEWMLDNFYLIEEQIRTAKRHLPKGYSRGLPR
                     LQKGPSAGLPRVYDIATEIVSHGDGRVDRENLTSFVTAYQTVTVLQLGELWAIPIMLR
                     LALIENLRRVATRIAVDRINRNHADYWADRITQTAEKDPKGLILVIADMARSNPPMVS
                     SFVAELTRRLQGQGPALALPLTWIEQQLSGSFLTIEQLVQTENQQQAADRLSVSNSIG
                     GLRFLSAIDWREFIEAMSAVEQTLRKDPCSVYGKMDFFTRDRYRHAVEEIAKISRLSE
                     SEVAREAIRLAQEGVGRKPGDDREAHVGFYLIDNGRAQLDRTAGVRYSAWESLQKACR
                     RYPFSLYLGTILFMTAILSGGLLAIAYFNGVYGFPLWCLGMLLVLSVSRLASGLVNWL
                     ATRLATPSPLPRMDFSKGIPPGELTLVVVPTMLTSTGSIEHLMDALEVRFLANRDENL
                     RFGLLTDFRDAAEGNLPGDETLSSLARRRIEALNQKYGGARGGTFFLFHRPRQWNPED
                     RIWMGYERKRGKLAELNSLLRGGSKANFSLIVGDVRALAKVKYVITLDTDTQLPRDAA
                     WQLVGAMAHPLNRPQYDETKGRISAGYGILQPRVAVSLPGTNRSRYARMWGSDPGIDP
                     YTQAVSDVYQDLFGEGSFIGKGIYEVDAFERVLKGRFPENRILSHDLIEGCFVRSGLI
                     SDVQLYEEYPSCYSADVSRRHRWTRGDWQILRWLLPGVPGRDARFQKNPLSPLSRWKI
                     FDNLRRSLTPPALTLLLLLGWTVLPLPWLWTLSVIGIIGVPALMSALVDLFQKPDDVL
                     LGPHLAGAARSIGRCLGQTVFTLLCLPYEAFFSMDAILRTAWRMLISDKLLLEWNPSG
                     DADRNRRTDLAGEFRIMWIAPVIAIAAGFRIVLSEPAVLPLALPILILWFASPMIAWW
                     ISRPLGQRAARLTPGQSILLRKISRKTWAFFETFVGPGDHWLPPDNYQEEPVAVIAHR
                     TSPTNMGLALLANLSARDFGYISTGQLMERTESAFRAMETLERHRGHFYNWYDTESLK
                     PLLPMYVSTVDSGNLAAHLLTLRVGLLALPDEKIAGAKVFDGLQDTLEALAEAAGETP
                     PGELAHLRRALAAACPGQADTLQVVREGLESVMAPALEAVRSFDSVPGSQAAWWAHAF
                     AGQCRAALDELAFLAPWTAQPPAQWPALTPLPEALIGFPGLGKIPTLRRLANLEEELL
                     PLIDDRYGDRRRPGKAPGEREELDALQALIVQASSRARARITAINELAVQCGEFARME
                     YEFLYDETRRLLAIGYNISEGRRDESYYDLLASEARLSCFVAIAQGKLPQESWFSLGR
                     LLTTVGGKPTLLSWDGSMFEYLMPLLVMPAYENTLLDQTCKAAVARQIEYGKNRGVPW
                     GISESGYHSTDAQLNYRYGAFGVPGLGLKRGLAGDLVVAPYATVLALMVAPEEACLNL
                     EELGRRGVEGRYGFYEAIDYTPTHLPRGQSRAVVRSFMAHHQGMSLLSLACLLLDRPM
                     QRRFESDLRFQATLLLLQERIPKATAFYSHATELSGMHTAVHGVSEMPVRVHTSPDTP
                     TPEIQLLSNGRYHVMITNAGGGYSRWKDMAVTRWREDGTRDNWGTFCYIRDRESGVFW
                     STAHQPALKVSNQYEAVFSEGRAEFRRRDHGFDTHTEIAVSPEDDIELRRITITNHGR
                     TRRSIDVTSYAEVVLASPAADALHPAFSNLFVQTEIIHRRHAVFCSRRPRSADEQTPW
                     MLHLMAVHGADIGEISYETDRLRFIGRGNNLDDPAAMRDMSALSGSDGPVLDPIVAIR
                     YQITLEAEQSATINVVTGIGETRDAALSLVEKYQDRRLADRVFDLAWTHSQVLLRQIN
                     AVEADAQLYGRLAASVLYANASLRAAPGVLLKNTRRQSGLWGYAISGDLPIVLLQIED
                     QANINLVRQLVQAHAYWRLKGLAVDLVIWNEDRAGYRQPLQEQITRLIAAGAGVNVTD
                     RSGGIFVRPADQISEDDRLLFQTVARVIIMDRRGTLTEQMNRRIPGESTVPALAPVRT
                     PRPEPPVVAALPRPDLMFFNGLGGFTPDGREYVISTARGQVTLAPWANVLANKHFGTV
                     VSENGLGYTWSENAHEFRLTPWYNDPVSDASGEAFYIRDEESGYFWSPMPLPSRGPTP
                     YVTRHGFGYSVFEHAERGISTEVWVYA"
BASE COUNT         1272 a         1987 c         1989 g         1231 t
ORIGIN      
        1 ggcgtaaacc cacacctccg tgctgattcc ccgctccgcg tgctcgaaca cgctgtagcc
       61 gaacccatgg cgcgtcacgt agggtgtcgg cccacggctg ggcagcggca tgggcgacca
      121 gaaatacccg ctctcctcgt cgcggatgta aaaggcctcc ccgctcgcgt cgctgacagg
      181 atcattgtac cagggcgtca ggcggaactc atgggcgttc tcactccaag tatagccgag
      241 gccgttctcc gagacaacgg ttccgaagtg cttgttggcc agcacattcg cccagggtgc
      301 cagcgtgacc tgcccgcggg cggttgagat gacgtactcg cggccgtccg gcgtgaatcc
      361 tcccaatcca ttgaaaaaca tcaggtccgg gcgaggcagc gccgctacga caggcggctc
      421 gggtcgagga gttctgacgg gtgcaagggc cgggaccgta ctttccccgg ggatgcggcg
      481 gttcatctgt tctgtcagcg tccctcggcg gtccatgatg atgacgcggg ctaccgtctg
      541 gaagagaagg cgatcgtcct cggagatctg gtcggcaggc cttacgaaga tgccgcccga
      601 cctgtccgtc acgttgaccc cggcgcccgc ggcaatgagc ctcgtgatct gctcttgcag
      661 aggttgccgg taaccggcgc gatcctcgtt ccagatcacc aggtccacgg ccagtccctt
      721 gagacgccag tacgcgtggg cctgaacgag ctggcgcacc agattgatat tggcctgatc
      781 ctcgatctgc agcagcacaa tggggaggtc tccggaaatg gcgtagcccc acagcccgga
      841 ctgccggcgg gtattcttga gaaggacgcc cggggccgca cgcagagagg cattggcata
      901 caggaccgag gcggcaagac gcccgtagag ttgcgcatcg gcttccacag cattgatctg
      961 ccggagaagt acctggctgt gcgtccacgc cagatcaaaa acgcggtcgg cgagccgccg
     1021 gtcctggtat ttctccacca ggctcaaggc cgcatcccgc gtttcgccga tgccggtaac
     1081 gacgttgatt gttgcggatt gctccgcctc cagggttatc tgatagcgga tggccacaat
     1141 gggatcgagt accggaccat cgctgcccga gagcgcggac atgtcccgca ttgcagccgg
     1201 gtcgtccaga ttgttgccgc ggccgataaa ccgcaggcgg tcggtctcat aggagatttc
     1261 cccgatgtcg gccccatgca cggccatcaa gtgaagcatc catggcgtct gctcgtccgc
     1321 agaacggggc cggcggctgc aaaagacggc atgcctgcgg tggatgatct cggtctgcac
     1381 aaagaggttg ctgaaggcgg gatggagcgc gtccgccgcg ggcgatgcaa gaacgacttc
     1441 ggcgtaactc gtgacgtcga tgctcctgcg cgtccggccg tggtttgtga tggtgatccg
     1501 gcgcagctcg atgtcatcct cgggggaaac ggcgatttcg gtatgcgtgt caaaaccatg
     1561 atcccggcgg cgaaactccg cccgcccttc cgagaatacc gcttcgtact gattcgacac
     1621 cttgagcgcc ggctgatgtg ccgttgacca gaagacgccg ctttctctgt cgcggatata
     1681 acagaatgtg ccccaattgt cgcgggtgcc gtcttcgcgc cagcgcgtca ccgccatgtc
     1741 cttccagcgg ctgtagccgc ctcctgcgtt tgtgatcatc acatgatatc tgccgttgga
     1801 cagcagctgt atttccggtg tgggggtgtc ggggctcgta tggacgcgca ccggcatttc
     1861 cgatacccca tgtacggctg tatgcatccc ggagagttcg gtcgcatgcg aatagaaggc
     1921 cgttgccttt ggtatccgct cctggagcag cagcagggtc gcctgaaacc ggaggtccga
     1981 ctcgaatcgc ctctgcatgg gacggtcgag cagcagacag gccagggaga ggaggctcat
     2041 accctggtgg tgggccataa aggatcgaac gacggcgcgc gattgccccc gtggcagatg
     2101 ggtcggagta tagtcgatgg cttcatagaa accgtatctc ccttcaaccc cgcggcggcc
     2161 gagttcctcg aggttcagac aggcctcttc aggcgctacc atgagcgcca gtacggtagc
     2221 atagggcgcg acaaccaggt ccccggcaag cccgcgtttg agccccaggc ccggcacgcc
     2281 gaacgcgccg tatcggtagt tgagttgcgc gtcggtcgag tgatagcccg attccgaaat
     2341 accccacggc acgccgcgat ttttcccgta ctcgatctgt cgcgccaccg cggccttgca
     2401 ggtctggtcc agcagcgtgt tttcataggc cggcatcacc aggagcggca tcaggtattc
     2461 gaacatcgag ccgtcccacg aaagaagggt tggtttcccc cctacggtgg tgagcaggcg
     2521 tcccagggaa aaccagctct cctggggcag ctttccctgg gcaatggcga cgaaacagga
     2581 gagtctcgcc tcggaggcca ataaatcata gtagctttca tcccgccggc cttcgctgat
     2641 attgtaaccg atggcaagca gacggcgggt ctcgtcatag aggaattcat actccatccg
     2701 ggcaaactcg ccgcattgca ctgcaagctc gtttatagca gtgattcttg cccgggcgcg
     2761 gctgctcgcc tgcacgatca gcgcttgcag cgcatcaagc tcttcgcgct ccccgggcgc
     2821 ctttccaggc ctgcgtctat ccccgtatct atcatcaatg agcggcagca actcttcctc
     2881 aagattggcc agccggcgca gtgttggaat tttgccgagg ccggggaaac cgatgagcgc
     2941 ctctggaaga ggcgtcagcg caggccattg tgcgggcggc tgtgccgtcc aaggcgcaag
     3001 aaaagccagt tcatcaaggg cggcccggca ctgcccggca aaggcatgcg cccaccatgc
     3061 tgcctggctc ccggggacac tgtcaaagct gcggaccgct tccagggcag gcgccatgac
     3121 cgactccagc ccttcgcgca caacttgcag agtatccgct tggccgggac aggcggcggc
     3181 cagcgctctc cgcaaatggg cgagttcccc gggaggggtt tctcccgcag cctccgcgag
     3241 agcctccagc gtgtcctgga ggccgtcaaa tacctttgcg cccgcgatct tctcgtcggg
     3301 gagcgccagg agccctaccc gcagggtcag caaatgggcc gcaagatttc cgctgtccac
     3361 cgtggaaaca tacatgggga gcagcggttt caaggattcg gtgtcatacc agttgtagaa
     3421 atggccccgg tgccgctcca gggtttccat ggcgcgaaag gcgctttccg tgcgttccat
     3481 gagttgcccc gtggaaatat aaccgaagtc ccgggcggac agatttgcca ggagcgcaag
     3541 ccccatgttt gtgggcgacg tgcggtgcgc aatgacggca acgggctctt cctgatagtt
     3601 gtcgggcgga agccaatggt ccccgggtcc cacgaaggtt tcgaagaagg cccaagtctt
     3661 gcgggaaatc ttcctcagta aaatgctctg gccgggggtg agcctcgctg cgcgctgacc
     3721 gaggggccga ctgatccacc aggcgatcat tggggaggca aaccagagga ttaagatcgg
     3781 caaggccagg ggaagaacag ccggctccga aagcacgatc cggaaccctg cggcaatcgc
     3841 gatgaccgga gcaatccaca tgatccgaaa ttccccggcg aggtccgtcc ggcgattccg
     3901 gtcagcgtcc ccggaggggt tccattcgag aagaagcttg tccgagatca gcatccgcca
     3961 cgcggtacgc agaatggcat ccatactgaa aaatgcctcg tacggcaggc aaaggagcgt
     4021 aaatacggtt tggccgaggc acctgccgat ggaacgcgcg gcgccagcga ggtgcggacc
     4081 caggagcaca tcgtcgggct tttgaaaaag gtcgaccagg gcgctcatca acgcggggac
     4141 cccgattatc ccgatcaccg acaaggtcca taaccacggc aacggcagca ccgtccagcc
     4201 cagaagtaaa aggagcgtta acgccggggg cgtaaggctg cgccggaggt tgtcaaaaat
     4261 tttccaccgg gacaacgggg agagcgggtt cttctgaaag cgcgcatccc ggccggggac
     4321 acccggcagc agccagcgca gaatctgcca atccccgcgg gtccagcggt gccggcggct
     4381 cacatcggcg ctgtagcagg aggggtattc ctcgtacagt tgcacgtcgc tgatgagccc
     4441 tgagcgcaca aagcaccctt cgatcagatc gtggctcagg atgcggttct ccgggaaacg
     4501 ccccttgagc acccgctcga acgcgtcgac ctcatagatt cccttgccga taaacgagcc
     4561 ctcgccgaaa aggtcctggt aaacatcgga gacagcctga gtgtagggat cgatgccggg
     4621 atcgcttccc cacatgcgcg cataccgcga ccggttcgtg ccgggcaggc tcacggccac
     4681 ccgaggctgc aggatgccgt atccggccga gatgcgccct ttggtttcgt cgtattgcgg
     4741 acggttcagc gggtgcgcca tcgcccccac cagttgccac gccgcatcgc ggggcaattg
     4801 tgtatccgtg tcgagggtga tgacgtattt taccttcgcc aatgccctga catcccccac
     4861 gatgagcgaa aagttggcct tcgagccgcc acggagaaga gaattcaact ccgccagctt
     4921 tccccgcttc cgctcgtacc ccatccagat ccggtcctcc ggattccatt ggcgcgggcg
     4981 gtggaaaagg aagaaggtac cgcctcttgc gccgccgtac ttttgattca acgcttcgat
     5041 tctccgccgg gccagcgatg acagcgtttc gtcgccggga aggtttccct cggcggcatc
     5101 gcgaaaatca gtgaggaggc cgaatcgcag gttttcatcc cgatttgcca ggaaacggac
     5161 ttccagggca tccatcaggt gttcgatgct cccggtgctg gtaagcatcg tcgggacaac
     5221 gacgagcgtc agttcgcccg gcgggatgcc tttggagaaa tccattcgtg gtaacgggga
     5281 aggcgtggcc agacgcgtgg cgagccagtt caccaggccc gaggctaacc ggcttacgga
     5341 cagaaccagg agcatgccga ggcaccacag gggaaaacca tataccccat tgaaataggc
     5401 tattgccagc aaccccccgc ttagaatcgc agtcataaac aggatggtgc caagatagag
     5461 ggagaacggg tatcggcggc aggctttttg aagggactcc catgcggagt atcttacgcc
     5521 cgcggtccga tcgagctgcg cacgcccgtt gtctatcagg tagaagccca catgtgcctc
     5581 ccgatcatcg ccgggtttcc gacccactcc ctcttgcgcg agccggatcg cctcgcgggc
     5641 cacctcactt tcggacagtc ggctgatttt cgctatctcc tccacagcat gacggtatcg
     5701 atcccgggtg aaaaagtcca tcttgccgta cacactgcaa ggatctttgc ggagggtctg
     5761 ctctactgcg ctcatggcct cgatgaattc gcgccagtcg atggcgctca ggaaacgcag
     5821 gcctccgatg ctgttgctga cggaaagccg atcggcggct tgctgctggt tttccgtctg
     5881 taccaactgt tcaatcgtca gaaatgatcc ggagagctgc tgctcgatcc aggtgagcgg
     5941 caaggcgagg gcgggtccct gcccctgtag gcgacgggtc agttccgcga caaaggaact
     6001 caccatgggc gggttcgacc gcgccatgtc cgcgatcacg aggatcaggc ccttcggatc
     6061 tttttcggcg gtctgagtga tccggtccgc ccaataatcg gcatggttcc gattgatccg
     6121 gtcaacggca atccgcgttg caacgcgacg gagattctca atcaacgcca gacgcagcat
     6181 gatggggata gcccataatt ctcccaactg caatacggtg accgtctgat aggctgtcac
     6241 gaaactggtc aggttttccc gatccacccg gccgtcgccg tgggaaacaa tctccgttgc
     6301 aatgtcatat acgcgcggaa gtccggccga cggtcccttt tgcaggcggg gcagtcctct
     6361 gctgtagccc ttcggcaaat gtctcttggc ggtacgaatc tgttcttcaa taagatagaa
     6421 gttgtcgagc atccattcgc cagctggcgt aatccgccaa ttggccttgg ctgcctcgg
//
LOCUS       DAOPBQ010000170         6439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10611_length_6439_cov_4.063753, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000170 DAOPBQ010000000
VERSION     DAOPBQ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10611_length_6439_cov_4.063753"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PKW60_06905"
     CDS             <1..620
                     /locus_tag="PKW60_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPJ99199.1"
                     /translation="AERGFIVYAPQNPYIGGDAFRVLQRKANPLKLSLFSFIVRQHER
                     TLEWLAEQPFVEPDRIGFYGISYGGKTAMRVPALLPQYALSICSADYNEWIWKNVSIE
                     FPFTYLYTGEYEMPEFDLGDTFNYAEMSWLICPRPFMVERGHHDGVSIDAWVAFEYAR
                     TQYLYSVLEHGGNTRIEYFNGPHAIHGVGTFEFLHDKLHWPEPGE"
     gene            674..2689
                     /locus_tag="PKW60_06910"
     CDS             674..2689
                     /locus_tag="PKW60_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99200.1"
                     /translation="MKRWLACISSILLAAALASAGEAPNLATNPGFEEVEAGSSLPAQ
                     WRGSDQVYMRDTAVARSGNASLKFQNADEGRYVLCTQPIELEAGVKYELSAWVKTENI
                     EGNESGATLCIEFYNAKGKYIGGAYPSGVTGTRDWTLVHGLTPRIPEDQAVSCSLACY
                     VRKGMTGTAWWDDVSVRRFREAPLSAVLKSPNYRDEITRDGPRDVIVFLELNLRDYEL
                     EPEDMAVHYAVYPENGDSPVLSGQMTSLTAPSFDISLRARKFKPGANRVEIALLEKSS
                     GETLATETLHVTRNDERVTRAVNIDEHNRLLVDGRPFFPLGMYWSEIDQEQLDVYADS
                     AFNCLMPYGMPTQEQMDMAQAKGLKVIYSVKDIYYGSSYCPKAITSREDESAFIRGKV
                     EAFQDHPALLAWYINDELPSSMLDRLTERRELLEQLDPGHPTWVVLYQVEELRKYLPS
                     FHAIGTDPYPIPSLPVALAGQWTRKTVDAVHGARPVWMVPQVFNWANYKKTADEKALY
                     RAPALEEMRSMAWQCIAEGANGLVFYSWFDLRKDPTTPFDEQWARVKKVAAGIAPLAP
                     VLLSVEDVPAIETSEELWLNWTAKKHEGVVYLIAVNNEPEKHVATFNLSKKIRKAIDV
                     PTREEWPLPDRRALELQFEPYEVHIVALHGMERAWNFGFSLNLDLTL"
     gene            2783..4057
                     /gene="aroA"
                     /locus_tag="PKW60_06915"
     CDS             2783..4057
                     /gene="aroA"
                     /locus_tag="PKW60_06915"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPJ99201.1"
                     /translation="MHLICEKSALRGAVTIPGSKSHTIRAVAVAALAEGESLIRRPLE
                     SRDAEACLAAYRAFGAELELTPELWRVQGLHGELCAPENVIDVGNSGTSLRIAMGSAA
                     LLRSGLAVLTGDQQIRQRPGGPLADALTQLGAQVRSTHGNGCAPFVVEGRLRGGQIAI
                     EAVTSQYLSSLLLAAPLADGSTEIEVTLLNEAPYVAMTLDWLERTGIVLEHDGLRWFH
                     IPGGQQYTAFDRLIPGDFSSATFFLAAGALEGNDIVSRGLDLADVQGDKAVLDYLRAM
                     GAEVTEEADGIRVRAKALKGVELDLNATPDALPMMAVLGCFAQGTTRLVNVPQARLKE
                     TDRIAVMRQELTRLGAQVEELDDGLVIHQSRLHGGDVDGQDDHRVVMALAVGATGIAE
                     PVRIRGYEAVSVTYPGFAEELRRLGGTARLAE"
     gene            4129..5460
                     /locus_tag="PKW60_06920"
     CDS             4129..5460
                     /locus_tag="PKW60_06920"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPJ99202.1"
                     /translation="MLGCTYGNMFKTTVAGGSYQEGLTVHLQGVPTGLRITEELIYRD
                     LLLRKPGQGELTSPRREPDVPVIYSGLNAADTMPGFHNQGKTNGTPLVVLIPNLDRHF
                     EHIEQYQVTNRTPRPGHASYASYQKYGEDDDSIGAGFFSGRYTSTIVAAGAVAKRVLK
                     DHGIDVFGYVKEAAGVRAADLPRDEARARVEAFRTVCRRHDVIYDWIFGAKRFRDDMR
                     FLEKMAVLAELEKNLIEIRNSAEPIDMEAVCREAGVHPLLNCPDVDAARAMYKRIVEI
                     RDEGDSSGGVCEVVATGVPVGVGEPVFSKLDGELGRMMSIGTIKAVEIGAGAKVKDMR
                     GSECNDQMRIRNGAAVFDSNNAGGITGGLTTGQDIVVRLTVKPTPTIAKDQATIDKVS
                     MEDATLSAVTRRDPTIVARVWPVAEAFMAIILLDQLAMHYGYRALQEKVTG"
     gene            complement(5517..5993)
                     /locus_tag="PKW60_06925"
     CDS             complement(5517..5993)
                     /locus_tag="PKW60_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPJ99203.1"
                     /translation="MTKLLPVHWRSSVDELRDRVVQTFDRWLPEDWRREPRIDMRDWA
                     LQLMARGGPTIDLEETDDEIVVTAELPGLDKDDFKVEVEGQRLILRGERKSAREKERR
                     QYHCCECSYGSFCRAIALPCEIDPAQSTANYRKGVLTVTMPKTPAAKARCVRVTID"
     gene            complement(6063..>6439)
                     /locus_tag="PKW60_06930"
     CDS             complement(6063..>6439)
                     /locus_tag="PKW60_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPJ99204.1"
                     /translation="PEKTQEQAAVPDTREEARTLAPPVDIFENRDGLVVVADLPGVGK
                     DDAEVRVENGILTLKATAKSELPGEPLRHEYELQNFFRQFQLSEEVDQDRIKAEMKHG
                     VLIINLPKREETKPRQITVQVS"
BASE COUNT         1267 a         2025 c         1978 g         1169 t
ORIGIN      
        1 tcgccgagcg cggttttatc gtttacgccc cgcagaatcc ctacatcggc ggcgacgcat
       61 tccgcgtgct ccagcgcaaa gccaatccgc tgaaactttc gctcttctcg ttcattgtgc
      121 gccagcacga gcgcacgctc gaatggcttg ccgaacaacc cttcgttgag ccggatcgca
      181 tcgggtttta cggtatctcc tacggcggca agacggccat gcgcgtccca gcgctgcttc
      241 cgcagtacgc gctgtccatc tgctccgcgg attacaacga gtggatctgg aagaacgtct
      301 cgatcgaatt tccgttcacg tatctgtata ccggggaata cgagatgcct gaattcgacc
      361 tgggcgacac attcaattac gctgagatga gctggctgat ttgtccgcgc ccgttcatgg
      421 tcgagcgcgg ccaccacgac ggcgtctcca ttgatgcgtg ggtcgccttt gaatacgcgc
      481 gtacgcaata cctgtactcc gttttggaac acggcggcaa caccagaatc gagtatttca
      541 acgggccgca tgccatccat ggcgtgggca cgttcgagtt tctgcacgac aaacttcact
      601 ggcccgaacc gggagagtaa gaagtggaac aagccggcca gcgcatggcg gctggaaatg
      661 aaaggggcat gaaatgaaac ggtggttggc gtgcatcagc agcatcctat tggcggcggc
      721 ccttgcttcg gccggcgaag cgccgaacct cgcaacaaat ccgggcttcg aagaggtgga
      781 agccggcagc agcctcccgg cccagtggcg gggcagcgat caggtataca tgcgcgacac
      841 ggcggtggcc cgttccggca acgcctcgtt aaaattccag aatgctgacg agggccgtta
      901 tgtgctgtgc acccagccga ttgagctgga ggccggggtc aagtacgaac tctccgcctg
      961 ggtcaaaacc gaaaacatcg aaggcaacga aagcggcgcc accctctgca tcgagtttta
     1021 caacgcgaag ggcaaataca ttggaggcgc ttacccttcc ggcgttaccg gcacgcgcga
     1081 ctggaccctg gtgcacggcc tcaccccgcg cattcccgag gaccaggccg tctcgtgctc
     1141 actggcatgt tatgtgcgca aaggcatgac cggcaccgct tggtgggatg acgtctctgt
     1201 ccggcgcttt cgggaggccc ccctttccgc cgtcctgaaa tcgcccaact accgcgatga
     1261 aattacccgc gacggccccc gcgatgtgat cgtctttctg gaactcaatc tccgggatta
     1321 tgagttggaa cccgaagaca tggccgtgca ctatgccgtc tatcccgaaa atggggactc
     1381 gccggtgctg agcgggcaga tgacgtcctt gaccgcgccc tccttcgata tttctctccg
     1441 tgcgcggaag ttcaaaccgg gggccaatcg cgtggagatc gcgctgctcg agaagagcag
     1501 cggcgaaacc ctcgccaccg agacccttca cgtaacgcgg aatgatgaac gcgtaacccg
     1561 tgcggtgaat atcgacgaac acaaccgcct gctggtcgac ggccgcccgt tcttcccact
     1621 gggcatgtac tggtcagaaa tagaccagga acagctggat gtgtacgccg acagcgcgtt
     1681 taactgcctg atgccgtacg gcatgcccac gcaggagcag atggacatgg cgcaggccaa
     1741 aggcctcaag gtgatctatt cggtcaagga catctattac ggcagttctt attgcccgaa
     1801 agccatcacg agccgtgaag acgagtctgc gtttatccgg ggaaaagttg aggcgttcca
     1861 ggaccatccc gccctactcg cgtggtacat caacgacgag ctgccgtcga gcatgctcga
     1921 ccgccttact gagcggagag aactgctcga acagctcgat cccggccatc ccacctgggt
     1981 ggtcctgtat caggtggaag aactgcgaaa atatctgccc agtttccacg ccatcggaac
     2041 cgacccctac cccattccca gcttgccggt ggcactggcg ggacaatgga cccgaaaaac
     2101 ggtggacgcc gtccacggcg cacgcccggt gtggatggtg ccgcaggtgt tcaactgggc
     2161 aaattacaag aaaacagccg atgaaaaagc cctgtaccgc gctccggcgc tcgaggagat
     2221 gcgatccatg gcgtggcagt gcatcgccga aggtgcaaac ggcctcgtct tttacagctg
     2281 gttcgacctt cgcaaggatc ccaccacgcc ctttgacgag caatgggccc gggtcaagaa
     2341 ggttgccgcg ggtatcgccc ccctggcgcc ggtgctgctg tccgttgaag acgtccccgc
     2401 gatcgaaacc agtgaggaac tctggctcaa ctggaccgcg aaaaaacacg aaggggtcgt
     2461 ctaccttatc gccgttaaca acgaacctga aaaacacgtg gcaacgttca atttgtccaa
     2521 aaaaattcgc aaagcgatcg atgtccccac tcgcgaagag tggcccctgc ccgaccggcg
     2581 cgcactcgag ctccagtttg agccgtacga agtccatatc gtcgcgttgc atggcatgga
     2641 acgcgcgtgg aacttcggct tctcgctcaa ccttgattta acactgtaaa cgcgttgacg
     2701 gtcattcgcg gacgcgttat tatcgcgact tgcagccgcc ggaggtggcg gcggttgtga
     2761 cggcggaggg gtggatgttc ccatgcatct gatctgtgag aaaagtgcgc tgcggggcgc
     2821 ggtgaccatt cccggttcga agtcgcatac cattcgcgcg gtagcggtgg ccgcgctggc
     2881 tgaaggcgaa agcctgatcc ggcgcccgct tgaatcgcgc gatgcagagg cctgcctggc
     2941 ggcataccgc gcctttggcg cggagctgga gctcaccccg gagctgtggc gcgtgcaggg
     3001 gctccacggg gagctctgcg cgccggaaaa cgttatcgac gtcggcaatt ccggcacgag
     3061 cctgcgcatc gccatgggtt cagcggccct gctgcgctcg ggactggccg tgctcacggg
     3121 cgatcaacag atccggcagc ggcccggcgg cccgctggcg gatgccctca cccagctcgg
     3181 tgctcaggtg cgctcgacac acggcaacgg ctgcgcgccc tttgtcgtcg aaggccgatt
     3241 gcgcggcgga cagattgcca tcgaggccgt gaccagccag tacctgtcca gcctcctcct
     3301 cgcggcgccg ctggccgacg gcagcacgga aattgaggtc accctgttga atgaggcccc
     3361 ttacgtggcg atgaccctcg attggctcga gcgcacgggc attgtcctcg agcacgacgg
     3421 gctccgctgg ttccacatac ccggcggaca gcaatatacg gcctttgacc ggctcatccc
     3481 cggcgatttc tcgtcggcaa ccttctttct ggccgcggga gcgctcgaag gcaacgacat
     3541 cgtgtcgcgg ggtctcgatc tggccgacgt gcagggcgac aaagccgtgc tcgactatct
     3601 ccgcgcgatg ggagcagaag tcacggaaga agccgacggg atacgcgtgc gcgcgaaggc
     3661 gttgaaaggc gtcgagctgg acctgaacgc cacgccggat gcgctgccca tgatggccgt
     3721 gctcggctgt tttgcccaag gcacgacgcg cctggtcaac gtcccgcagg cgcgtctcaa
     3781 agaaaccgac cgcatcgccg tgatgcgcca ggaactcacg agactcgggg cgcaagtcga
     3841 ggaactcgat gacggcctgg tgattcatca gagccgcctg cacggcggcg atgtggacgg
     3901 gcaggacgat caccgcgtgg tgatggcgct ggccgtgggc gcgaccggca tcgcggaacc
     3961 ggtgcggata cggggctacg aagcagtctc cgtcacctac cccgggtttg cagaagaact
     4021 gaggcggttg ggcggaacgg cgcgccttgc ggaatagcag gatcgccgcc gatctgccgg
     4081 agcgcgtgac aatagccaga aacacaagaa catgaggaga gagaacccat gctcggctgc
     4141 acctacggca atatgttcaa aaccacggtg gcgggcggct cgtaccagga aggactgacg
     4201 gtacacctgc agggcgtgcc caccggcctc cgcatcaccg aagagctgat ctaccgcgac
     4261 ctgctgctgc gcaaacccgg ccagggcgaa ttgacatcgc cccggcgcga acccgatgtg
     4321 ccggtgatct acagcggcct gaatgcggcg gacaccatgc cgggattcca caaccagggc
     4381 aagaccaacg gcacgcccct ggtcgtgctg atccccaatc tcgaccgtca cttcgagcac
     4441 atcgaacagt accaggtcac caaccgcacg ccgcggcccg gccacgcctc gtatgcctca
     4501 tatcaaaaat acggcgagga cgacgactcc attggcgccg ggttcttcag cggccgatac
     4561 acctccacga tcgtcgccgc gggcgcggtg gcgaaacgtg tgctcaaaga ccacggcatc
     4621 gacgtgttcg gctacgtaaa ggaagccgcg ggcgtccggg ccgcagattt accccgcgac
     4681 gaggctcgcg ccagggtcga agcatttcgg accgtgtgcc gccggcacga tgtcatctac
     4741 gactggatct tcggcgcgaa acggtttcgc gacgacatgc ggtttcttga aaagatggcc
     4801 gtgctcgcag aactcgaaaa aaacctcatc gagatccgca acagcgctga acccatcgat
     4861 atggaggccg tctgccgcga ggcgggcgtc catcccctgc tgaactgccc ggatgtcgac
     4921 gccgcgcggg ccatgtacaa acggatcgtc gaaatccggg atgaaggcga ttccagcggc
     4981 ggggtgtgcg aagtggtggc gaccggcgtg cccgtgggtg tgggtgaacc cgtcttctcc
     5041 aagctcgatg gcgaactggg ccgcatgatg agcatcggca ccatcaaagc ggtcgaaatc
     5101 ggcgctggag cgaaggtaaa agacatgcgc ggctcggagt gcaacgacca gatgcgtata
     5161 cgcaacggag cggcagtctt cgacagcaat aacgcgggcg gcatcaccgg aggcctgact
     5221 acggggcagg acatcgtcgt gcggctgaca gtcaaaccca cgccgaccat tgcgaaggac
     5281 caggccacga tcgacaaggt ctcgatggaa gacgccacgc tgtccgccgt gacgcgccgc
     5341 gatcccacga tcgtcgctcg cgtatggccg gtggccgagg cattcatggc catcattctg
     5401 ctcgaccagc tggccatgca ctacggctac cgcgcgctgc aggaaaaagt caccggctga
     5461 gccgttgcgc cattaccctg gcgttgcgcg agtaatctgt ccggttgttt gcccgctcag
     5521 tctattgtta cacggacgca gcgggccttg gccgccggcg tcttgggcat cgtcaccgtg
     5581 agcacgcctt tgcggtagtt ggctgtgctc tgggccggat cgatctcgca ggggagcgct
     5641 atggcccggc aaaacgagcc gtagctgcat tcgcagcaat gatactggcg ccgttctttc
     5701 tcgcgcgcgg atttccgctc gccgcggaga atcaggcgct gtccctcaac ctccaccttg
     5761 aaatcgtctt tatcgaggcc cggcagctcc gccgtcacca cgatctcgtc gtcagtctcc
     5821 tccagatcga tggtcgggcc gccgcgggcc atgagctgga gcgcccaatc gcgcatatca
     5881 atgcgcggtt cacgccgcca gtcttcgggc agccaccggt cgaaggtctg caccacgcga
     5941 tcgcggagtt catcgactga gctgcgccag tgtaccggga gcagttttgt catggcgcgt
     6001 cacctccgtc aaacacgggc gccggccggg cggcgagctc tggccgcccg gcgcacccgt
     6061 cctcacgaaa cctgtacggt aatctgtctg ggcttggtct cctcacgctt cggcaggttg
     6121 atgatcagca cgccgtgctt catttcggcc ttgatccgat cctggtcgac ttcttcgctg
     6181 agctggaatt ggcggaagaa gttctgcagc tcgtactcgt gccggagcgg ctctccgggc
     6241 agttcgctct tggccgtggc cttgagcgtg agaatgccgt tctcaactcg cacctcggcg
     6301 tcatccttgc ccacgccggg aaggtcggcc actacgacca gcccgtcccg gttctcaaaa
     6361 atatccaccg gaggcgccag cgtccgcgcc tcttcccgtg tgtcaggcac tgcggcctgt
     6421 tcctgggtct tttccggta
//
LOCUS       DAOPBQ010000171         6430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10626_length_6430_cov_5.593255, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000171 DAOPBQ010000000
VERSION     DAOPBQ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10626_length_6430_cov_5.593255"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PKW60_06935"
     CDS             complement(<1..452)
                     /locus_tag="PKW60_06935"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPJ99205.1"
                     /translation="MWEAIRANKRKSVFLAAAMFMLMVGLGFFIGVSLFPASMDIDVD
                     GQTYSVPLFGGVLGALAACGVWALLTVTALFSGGAILMAASGARKIEKEDHPQLFNVV
                     EEMTIASGLGSMPAVYIIDDPALNAFATGRDPQHAAVAITSGLLSRLSR"
     gene            complement(523..1086)
                     /locus_tag="PKW60_06940"
     CDS             complement(523..1086)
                     /locus_tag="PKW60_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPJ99206.1"
                     /translation="MSILVVLIVLAVIVFGALAWLTGIYNGLVRLRNEVKNAWAQVDV
                     QLKRRYDLIPNLIETVKGYAKHERETFEKITQARSAAMSAQGVAEQAKAETQLSQALG
                     GLRLVVEAYPDLKANTNFLALQEELTSTENKIGFARQFYNDQVMQLNNKVQMFPSNIV
                     AGMFGFKLEDFFELEEPEAREAPKVQF"
     gene            complement(1154..1573)
                     /locus_tag="PKW60_06945"
     CDS             complement(1154..1573)
                     /locus_tag="PKW60_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HPJ99207.1"
                     /translation="MNCPACNEPMIGLEYDKVEVDYCLECAGVWLDEGELELLLGMDA
                     PEVTSMISNGEDIAAGEAKRSCPACDRPMVKMVFGAENRVVYDWCPHNHGLFLDRGEL
                     VAILEHAPGFERGREISEFLRGIFTEQEEKPRQVPEA"
     gene            complement(1749..2744)
                     /locus_tag="PKW60_06950"
     CDS             complement(1749..2744)
                     /locus_tag="PKW60_06950"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HPJ99208.1"
                     /translation="MTDSAGQRPLLEVDGLVKYFPVRGGVLSRVLGTVRAVDGVSFAI
                     PKGKTLSLVGESGSGKTTAGRSILRLIEPSAGRVLFNGVDLTKVDRRAMRRYRKAMQI
                     IFQDPYGSLNPRMTVYNMLSEILSTHKLVPRAQRRQRIDELLELVGLPPESADRYPHE
                     FSGGQRQRIGIARALAVEPELIVADEPISALDVSIQAQILNLLRDLQKRLGLTYLFIA
                     HDLSVVRHISDYVAVMYLGRIVERGPVDEIFAHPVHPYTKALLAAAPVPNPRRARRRQ
                     PLAGDVPSPINPPPGCHFHPRCPDAVAACQVSEQRLVEVTPGHWVACSVHAPYVE"
     gene            complement(2741..3769)
                     /locus_tag="PKW60_06955"
     CDS             complement(2741..3769)
                     /locus_tag="PKW60_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ99209.1"
                     /translation="MDTALSIRNLKTSFFTDAGIAKAVDGVSLDIPKGKTVALVGESG
                     CGKSVTALSVLRLVPDPPGRIVDGEIWFGGRNLVELPEREMRRIRGNGISMIFQEPMT
                     ALNPVFRVGRQIAAAVRVHRDVTKREAMDQAVDLLGQVGIPDPEQRVREYPHQMSGGM
                     RQRAMIAMALACNPELLIADEPTTALDVTIQAQILELLRRLQAERHMAILLITHDLGI
                     VAETADDVAIMYAGKIVEQAPVNDLFDNPRHPYTLGLFKSLPRLDEPRGRLRTIEGRV
                     PAATHFPPGCRFHPRCDYAMALCREQEPLLTEGAPGHHVACWLYDAEATGREGRPVGI
                     PRNEEAAP"
     gene            complement(3776..4162)
                     /locus_tag="PKW60_06960"
     CDS             complement(3776..4162)
                     /locus_tag="PKW60_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPJ99210.1"
                     /translation="MRIEHLALNAANPAELTGWLCDHLGMRVLRQFGEPPNAFFVADS
                     EGHTVLEIYNNPDGPIPDYASMHPATLHLAFVTDDVAGTLERLLAAGAAPETDVTTTP
                     GGDELAMVRAPGGLAIQLMKRAAPFI"
     gene            complement(4182..5213)
                     /locus_tag="PKW60_06965"
     CDS             complement(4182..5213)
                     /locus_tag="PKW60_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ99211.1"
                     /translation="MNGAAGKRQSYWRLVWRQYRKNRLAMAGLAVILCLFVVALLAPF
                     LAHSRPVYLVKDGHTYWFANLINYPQLVEVDFVNWTPGDGERALRPPVPFGPLDDNVL
                     QRLEPPALAGAGGTAGSSRHWLGTDDRGRDVLSRLIWGTRISMSIGFVAVGISALIGV
                     VFGALAGYYGGKVDLVILRLIEIVMCFPSFFLILTLMAFLPPSIWNIMVAIGLLGWTG
                     IARLVRGEFLRLRESDFATAARATGLHDGWIIFRHLLPNALAPVWVSATFGVAGAILT
                     ESGLSFLGFGVPPPAASWGEVLKQSQTYITYAWWLVTFPGAAIFITVTAFNLVGEGLR
                     DAMDPRLRQ"
     gene            complement(5223..6227)
                     /locus_tag="PKW60_06970"
     CDS             complement(5223..6227)
                     /locus_tag="PKW60_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ99212.1"
                     /translation="MKAYLIRRLFLVIPTFLGISMISFAVIQLAPGSPIYMKLRTAEQ
                     GISTDANTQEILRQTRELYGLDRPLPVQYIKWLGRLMTFDFGESFKDHRPVRDKILEA
                     LPITLQLNIISIFLVYLISIPIGVYSSTHQRTWPDTFITIGLFVLYSMPSFWVAMLLI
                     YFFGGGEWFNWFPVSGMNSIGAENFSWMQWLVDRTWHLVLPVFCLTYGGLAGLSRYAR
                     SGMIEVIRQDYIRTARAYGFSEKVVVFKYAMRNSLIPIITLLGTLLPALLGGSVIIES
                     IFSVPGMGFRGFDALLSRDYPMIMGLLAVSALLTLIGLLLSDILYAVADPRIKFEQSD
                     "
     gene            complement(6243..>6430)
                     /locus_tag="PKW60_06975"
     CDS             complement(6243..>6430)
                     /locus_tag="PKW60_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HPJ99213.1"
                     /translation="EERIRMYHRFHEILHEEQPYTFLFCSKSLLAVDKRFHGIVMYPL
                     GPDTREWWVPKALQRYP"
BASE COUNT         1295 a         2038 c         1944 g         1153 t
ORIGIN      
        1 cggctcagcc gcgagagcaa acctgacgta atagccaccg cggcgtgctg ggggtcgcgg
       61 cccgtggcga acgcgttcag ggcgggatcg tcgatgatgt acacggccgg catcgaaccc
      121 agaccggagg ctatcgtcat ctcctccacg acgttgaaca gctggggatg atcctccttt
      181 tcgatcttcc gtgcaccgct cgccgccatc aggatggcgc caccgctgaa aagggcggtg
      241 acggtcagga gtgcccagac accgcacgcc gccagcgccc ccaatacacc accgaataac
      301 ggtacggaat aggtctggcc gtcgacgtct atatccattg aagccgggaa cagggacacg
      361 ccgataaaga aacccagccc caccatcagc atgaacatgg ctgcggcgag aaagaccgat
      421 tttcgcttgt tcgcccgaat ggcttcccac attggcgccg ctatcctctt gcatacagcc
      481 cggaggcgct tccgggccgg ggttcacgaa tcggccgtgc tcttaaaact gcactttggg
      541 ggcttcacgc gcttcgggtt cttccagttc gaagaagtcc tccagcttga atccaaacat
      601 ccccgcaacg atattgctgg ggaacatctg caccttgttg ttcagttgca tcacctggtc
      661 gttgtaaaac tggcgcgcaa acccaatctt gttctcggtt gacgtcagct cctcctgcag
      721 ggcgaggaag ttcgtgttgg ccttcaggtc gggataggcc tcgacaacga gccgcagccc
      781 gccgagggcc tggctgagct gagtctccgc tttggcctgt tcggccacgc cctgcgcgct
      841 catggccgcg ctgcgcgcct gtgtaatctt ctcaaaggtc tcgcgttcgt gtttcgcgta
      901 gcccttgacg gtttcgatca ggtttgggat caggtcgtag cgccgtttga gctgcacatc
      961 cacctgggcc caggcgttct tcacctcgtt gcgcagccga accaggccgt tgtagatgcc
     1021 ggtcagccag gccagcgcgc cgaatacgat gaccgccagc actatcagaa cgacgaggat
     1081 actcatgggt gactcactcc ctcgttgtgc cacgcccgtt tcatccggac gcggctgccc
     1141 tcatccgcct catctatgcc tcgggaacct gcctcggttt ctcttcctgt tctgtaaaga
     1201 tgccgcgcag aaactccgat atttcgcggc cacgctcaaa tccgggcgca tgttcgagaa
     1261 tcgccacgag ttcgccccgg tcaagaaaca ggccgtggtt gtgcgggcac caatcgtata
     1321 ccacccggtt ttccgcgcca aacaccatct tgaccatggg ccggtcgcag gcgggacagg
     1381 aacgtttcgc ttcgcccgcc gcgatgtctt caccgttcga gatcatggac gtcacctccg
     1441 gcgcgtccat gcccagcaag agttccagct cgccctcgtc cagccacacg cccgcgcatt
     1501 cgaggcagta gtccacctcc accttatcgt attcaagccc gatcatcggc tcgttgcatg
     1561 cggggcaatt catacgtttt ctcctcaata gtcgcggccg cattctatac tcagtcgctc
     1621 ctcaattgcg aaaagaaacg ctggaaggca gcggaccggt ttgactctcg tccagagact
     1681 gcttggtggc ctccggcacg aggttgcccc tgtcggcacc ggatttgtgg ttaacaggcc
     1741 gggcgccgct attccacgta cggggcatgg acgctgcagg cgacccagtg gccgggcgtc
     1801 acctcgacca ggcgctgctc actcacctgg cacgccgcaa cggcatccgg gcagcgcgga
     1861 tggaaatggc agcccggggg cggattgatc ggcgacggaa cgtcaccggc gaggggctgg
     1921 cgccgccgcg cacgcctggg gttgggcacg ggcgccgcgg caagcagcgc tttcgtatag
     1981 ggatgcacgg gatgcgcgaa gatttcgtcg acgggtccgc gctcaacgat gcgccccagg
     2041 tacatcacgg cgacataatc ggagatgtgg cgcaccacgc tcaggtcgtg cgcgatgaac
     2101 aggtaggtca ggccgagccg cttctgcaga tcacgcagca ggttgaggat ctgcgcctgg
     2161 atcgagacat cgagcgcgga aatgggctca tcggccacga tcagctcggg ttcgaccgca
     2221 agcgcgcggg caatgccgat gcgctggcgc tggccgccgc tgaactcatg cgggtaccga
     2281 tcggcgcttt ccggcggcag cccgaccagt tcgagaagct cgtcgatgcg ctggcggcgt
     2341 tgcgcccgcg gcacgagctt gtgcgtggac aggatttcgg acagcatgtt gtagacggtc
     2401 atgcgcggat tcagcgaacc gtaagggtcc tgaaaaatga tctgcatggc cttgcgatag
     2461 cggcgcatcg cgcgccgatc gaccttcgtg agatcgacgc cgttgaacaa tacgcgcccc
     2521 gcggacggct caatgagccg cagaatgctg cgtccggcgg tggtcttgcc gctgccgctc
     2581 tcgccgacaa ggctcagggt cttgcctttc gggatcgcga agctgacccc gtcgaccgcg
     2641 cgaaccgtcc ccagcacacg ggacaacacg ccgccccgca cggggaagta tttgaccagg
     2701 ccatccactt caagaagagg ccgctgtcca gcggagtcgg tcatggagca gcctcctcat
     2761 tccggggaat acccacggga cggccctcgc ggcccgtggc ttccgcatca taaagccagc
     2821 aggcaacgtg atgcccgggc gcgccctccg tgagcagcgg ttcctgttcg cgacacaggg
     2881 ccatggcata gtcgcatcgc ggatgaaaac ggcagcccgg cgggaaatgc gtggcggcag
     2941 gcacgcgccc ttcgatcgtg cgcaggcgcc cgcggggttc gtcgaggcgg ggaaggctct
     3001 tgaacagccc cagcgtgtac ggatgccgcg ggttgtcgaa caagtcgttg accggcgcct
     3061 gttcgacgat ttttcccgca tacatgatcg ccacgtcatc ggccgtttcg gcgacgatgc
     3121 cgaggtcgtg cgtgatgagc aaaatggcca tatgccgctc ggcctggagc cggcggagca
     3181 gctccagaat ttgtgcctga atggtcacgt ccagggcggt tgtgggctcg tccgcgatga
     3241 gcagttccgg attgcacgcg agagccatgg cgatcatggc ccgctgccgc atgccgcccg
     3301 acatctgatg tggatattcc cggacccgct gttccgggtc cgggatgccg acctggccaa
     3361 gcagatcgac ggcttgatcc atcgcctctc tcttcgtcac atcgcgatgg acccgcaccg
     3421 ccgcggcaat ctgtctgccg acgcgaaata ccggattcag cgccgtcatg ggctcctgga
     3481 agatcatcga gatgccgtta ccgcgaatgc ggcgcatctc gcgctccggc agctccacca
     3541 ggttgcggcc gccaaaccag atctccccat ccacgatgcg gccaggaggg tcgggcacaa
     3601 gccgcaacac ggacagcgcg gtcacgctct tcccacaacc gctctctccg accagtgcga
     3661 ctgtcttgcc ctttggaatg tcgagagaca ccccatccac cgccttcgcg atgcccgcgt
     3721 cggtgaaaaa actggttttc aggttgcgga tgctgagtgc ggtgtccatg gtcttctaaa
     3781 tgaacggagc agcgcgtttc ataagctgga tcgcgaggcc gcccggagcg cgtaccatgg
     3841 ccagttcatc gcctcccggc gtcgtggtga cgtccgtttc gggcgccgcc ccggcggcga
     3901 gcagtcgttc cagggtcccc gccacgtcgt cggtcacgaa cgccaggtgc agtgtggccg
     3961 gatgcatcga cgcgtaatcc gggatggggc cgtccgggtt attgtagatc tcgagcacgg
     4021 tgtgtccttc ggaatcggcc acaaagaagg cgttcggcgg ctcgccaaac tgccgcaata
     4081 cgcgcatgcc gaggtggtca cacaaccacc cggtcaattc cgccggattg gctgcattca
     4141 gggccagatg ttcgattcgc atcgctcgct ccttcgtttc gttattgccg gagccgcgga
     4201 tccatggcat cgcgcagccc ctcccccacc aaattgaacg cggtcacggt aataaagatg
     4261 gccgcgcccg gaaaggtcac cagccaccac gcgtacgtga tgtaagtttg cgattgtttc
     4321 agcacttcgc cccagctggc cgcgggcggc ggcacgccaa accccagaaa actgaggccc
     4381 gattccgtca aaatagcacc cgcaacgccg aaggtcgcgg acacccagac gggcgcgagc
     4441 gcgttcggca acaagtggcg aaagatgatc cagccgtcgt gaagtcccgt agcccgcgcg
     4501 gccgtcgcga aatcactctc acgcaggcgg agaaactcgc cccgaacgag ccgggcgatc
     4561 cccgtccagc ccagcagccc gatcgccacc atgatgttcc agatgctggg cggcaggaag
     4621 gccatcaagg tcaggatcag aaagaagctg ggaaaacaca tcacaatctc gatcagccgc
     4681 agaataacca aatcgacctt accgccgtag taccccgcca gagccccgaa gaccacgccg
     4741 atgagcgccg aaatgcccac ggccacaaac ccgatgctca tcgaaatgcg cgtgccccag
     4801 atgagacggc tgagcacatc gcggccgcgg tcgtcggtgc ccagccaatg ccgcgaggaa
     4861 cccgcggtcc ccccggcgcc cgccagggcg ggcggttcga ggcgctgaag cacgttgtca
     4921 tcgagcgggc caaacggcac gggcggccgc agggcgcgct cgccatcacc gggggtccag
     4981 ttcacaaaat cgacctcaac cagctgggga tagttgatca ggttggcgaa ccagtacgtg
     5041 tgcccgtctt tgacaaggta tacgggacgc gaatgcgcca gaaacggcgc cagcagcgcc
     5101 accacgaaga ggcagagaat taccgccagc ccggccatgg cgagccggtt tttccggtac
     5161 tgccgccaga ccagccgcca gtaactctgt ctctttcccg ctgcgccgtt cataccgtcc
     5221 tgtcagtccg attgttcaaa tttgatgcgc ggatccgcca ccgcatacaa aatatcggac
     5281 agaagcagcc cgatgagggt gagcaacgca gataccgcca acagacccat gatcatcgga
     5341 taatcccgcg acaaaagcgc gtcaaacccc cggaaaccca tcccgggaac ggagaaaatg
     5401 ctctcgatga tcacgctgcc gcccaacaag gcgggaagca gcgtaccgag cagcgtgatg
     5461 atcgggatca gcgagtttcg catcgcgtat ttgaagacca cgaccttttc cgaaaatccg
     5521 taggcgcggg cggtgcggat atagtcctgg cggatgacct caatcatccc cgagcgggca
     5581 tagcgcgata atccagcaag cccgccgtag gtcagacaaa agaccggcag caccaggtgc
     5641 caggtgcggt ccacgagcca ttgcatccac gaaaaattct ctgcgccaat cgagttcatg
     5701 ccggagaccg ggaaccagtt aaaccattcc ccgccgccga agaagtaaat cagtagcatc
     5761 gccacccaga agctcggcat gctgtagagc acgaagagcc cgatcgtgat aaacgtatcc
     5821 ggccaggtgc gttggtgggt ggaggaatac actccgattg gaatggagat gagatagacg
     5881 agaaaaatcg aaatgatgtt gagctggagc gtgatcggca gcgcttcgag gattttgtcg
     5941 cgcacggggc ggtggtcttt gaacgattcg ccgaagtcga aggtcatgag gcggcccagc
     6001 catttgatgt attgcacggg aagcggccgg tccagcccgt agagctcccg ggtctgccgg
     6061 agaatttcct gggtgttggc gtcggtggaa atgccctgct cggccgtgcg cagtttcatg
     6121 tagatcgggc tgccgggcgc caattgaatg accgcgaacg aaatcatgct gatcccgaga
     6181 aaggtcggaa tcactagaaa caagcgtcgt atgagatagg ctttcacaaa ccacccatta
     6241 cgttacggat accgctgcaa tgccttgggt acccaccact cccgcgtgtc ggggccgagc
     6301 ggatacatca cgataccgtg gaaacgcttg tccacggcca gaagcgattt gctgcagaaa
     6361 aggaacgtgt agggctgttc ctcgtggaga atctcgtgga accggtggta catccggatg
     6421 cgctcctccc
//
LOCUS       DAOPBQ010000172         6425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10645_length_6425_cov_3.859969, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000172 DAOPBQ010000000
VERSION     DAOPBQ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10645_length_6425_cov_3.859969"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="PKW60_06980"
     CDS             complement(<1..1515)
                     /locus_tag="PKW60_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPJ99214.1"
                     /translation="MELTRLQIANYKGLRDIDVPMSRFGCLIGENNSGKSSVLQALSL
                     FFSGSAILQTHFFDPSLPIRIAVTFSEISEDDLAKLAEEHRAKIEPLITNETLTLVRF
                     YSTDGKSTMRYQTLVPRAERFQADKIAALVKGQKPGKNFMEAVTTIFPELKETMTIHN
                     SQGEVKVLIKQLADSLPAEQKQLADIDLPTGIDKSVTPFLPEPIYIPAVKDLSDDVKT
                     KEGTPFGKILRILLKAIEPDLAEAQEVFEELNKRLNRIVLPDGKTEDNRLEQVRAIER
                     TIEKYVQESFSNVFVEIDIPPPDIKTVLSSARIYADDGIRSEIDSKGDGLRRAAAFAI
                     LRSYVDLNRLDDLTKPPIESARPNEHILLFEEPELYLHPRAQQILFVALGLFSKRHSV
                     LVTTHSPLFFGPDATATFIKLVKRRDLALSTKPFACVHPIDLSDMNAKDQFQLICYEN
                     NNAAFFAQTVVLIEGDSDYLVFPHIMRLIRPQKDFTNAGVFFVRIGGKGSIRRYR"
     gene            complement(1641..1910)
                     /locus_tag="PKW60_06985"
     CDS             complement(1641..1910)
                     /locus_tag="PKW60_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99215.1"
                     /translation="MEREIKELDKEIRETKKLSVLAASLRDKLEAQKQVKALESARNK
                     KRCELFYAQDQIDDQRNKLIAGLERQLKQIVASKPLFAVRWTIEG"
     gene            2786..4834
                     /locus_tag="PKW60_06990"
     CDS             2786..4834
                     /locus_tag="PKW60_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPJ99216.1"
                     /translation="MNAFLAGLNPAQREAVTHTEGPVLILAGAGSGKTRVITQRIAHI
                     LARGIAQPGEILAVTFTNKAAGEMRERVAALTGAQAARDLVISTFHSFCLRVLRAEIT
                     HLGYRKDFTISSEGDARTMVRRLLDNLDGVRETFDPDSFLEQIGWVKNSGLFEVPENQ
                     PEGDTDTARKYATWLPAVFEKYQSALRAANTLDFDDLLLLTLRLWREHPRILARYQKR
                     LRYVMVDEYQDTNPLQYELLRLLASAHRNLCVVGDDDQSIYGWRGADIQNILSFEKDY
                     PDARIVTLEQNYRSTQTILSAANAVIANNRARREKRLWSELGAGRPLDWFVTADEEHE
                     AKMIVSWIQHIRDRTRAKYTDFAVLYRSNLQSRPIEIAMRQAKIPYVVVGGQDFFERT
                     EVKDIIAYLRAVVNPRDEAAFLRIANMPRRGIGDATLHQVHALCLAENLPFAKGLSEA
                     MRRGLIQGRAEAGIRELSGLLRGFGERFRQEGVSLTQQVQELVDRIGYRAELVRTSKS
                     DLQLEQKWSNVEAVIDAVAQYEQQSPVPPTLNGFLDESALNKTEDHREDGRQQRSGVT
                     LMTIHSAKGLEFPFVLIAGIEEGLLPHEKCLQGAALEEERRLFYVALTRAKRHATLFE
                     SCVRSRRGRERLSTTSRFIKEIPETLLCRHVRAAREMIAERTAPAQASQSPKRRRRSK
                     "
     gene            complement(4792..5823)
                     /locus_tag="PKW60_06995"
     CDS             complement(4792..5823)
                     /locus_tag="PKW60_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPJ99217.1"
                     /translation="MKRCRGSLITAGLMVVSVAVVLASIGVGSESIHFGAAWRDWRAG
                     RPLADAPELAILLQHRLPRTLAALLAGGGLALAGCVFQALLRNPLATPYTLGVASAGA
                     FGAYTATVVMRSAGLVLTLGGFSAVQAFAFVFALLDVSLIYLIAVRRHHVSPPVLLLA
                     GITLGMLSNAGMMLVRFLSDPGMLAMMERWLMGGVDIIGYEPVATLAMGVLPCSMVLL
                     LQAAQFDQLGFGAEIAQSRGVNVRRLQTTTFLLGSFLTAVVVSEVGPIGFVGLIVPHA
                     VRSLTGSRHRLLMPLSFLAGGAFLCLCDIFARIVLPGETPIGIITAFVGGPFFLYLLL
                     RRRFGDWEA"
     gene            complement(5820..>6425)
                     /locus_tag="PKW60_07000"
     CDS             complement(5820..>6425)
                     /locus_tag="PKW60_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ99218.1"
                     /translation="PLSALHNRERARILAFLPQTVAPAFTLTAAEVVILGRYPHLGPL
                     GTPSGRDREIVLRCMRETETEHLRHRDFGTLSGGERQRVLLASILAQEPDLLLLDEPT
                     AALDIHHQAGIFSLLHGLARNGYGVAVVTHDLNVAGQFCDRLVLLSSRARIVAQGTPP
                     EVLTPDLLSEAYESSIVVTEHPITGTPLVWPEPASVREGAP"
BASE COUNT         1393 a         1842 c         1915 g         1275 t
ORIGIN      
        1 tcgataacgg cgaatgcttc ccttgcctcc aatgcgaaca aaaaaaacac ctgcgttcgt
       61 gaagtctttt tgaggccgga taagcctcat tatatgtggg aacaccagat agtcgctgtc
      121 accctcgatg aggaccacgg tttgggcaaa gaacgcggca ttattgtttt cataacagat
      181 gagctgaaat tggtcttttg cgttcatgtc gcttaagtca atggggtgaa cacaggcaaa
      241 tggctttgtt gagagggcta aatcacgcct tttcacaagt ttgatgaatg ttgccgttgc
      301 gtccgggccg aagaagagag gtgagtgggt ggtgacaaga acggaatgac gttttgaaaa
      361 gaggccgagt gcgacaaaga ggatctgctg cgccctagga tgtaaataga gttcgggctc
      421 ctcaaaaagc aggatgtgtt cattcgggcg ggcggattcg attggaggct ttgtcaaatc
      481 atcgagtcgg ttcaaatcaa cgtaggaacg aaggatagcg aatgctgcgg cacgacgcaa
      541 cccatcgccc ttactgtcaa tctcgcttcg aatgccgtcg tcggcataaa tgcgggcaga
      601 ggaaagcact gtcttaatgt cgggtggtgg aatatctatt tcgacgaaaa catttgaaaa
      661 actctcctgg acatatttct caatagtccg ttcaattgcg cgcacttgct cgagacgatt
      721 atcctccgtt ttcccatcgg gcaaaacaat cctgtttagc cgcttgttaa gttcctcgaa
      781 aacttcttgc gcctcagcca aatctggttc gattgccttg agtagaatgc gcaggatttt
      841 gccaaacggt gtaccttctt ttgtcttaac gtcatctgac aagtctttga ccgctggaat
      901 gtagatgggc tcaggaagaa atggtgtgac acttttatca atgcctgtag gaaggtctat
      961 atcggccaac tgtttttgtt cggcggggag actgtcagca agttgtttga taagcacctt
     1021 cacttcccct tgactgttgt ggatggtcat tgtctctttg agttcgggga agatggttgt
     1081 gacagcttcc atgaaattct tgcccggttt ttggcctttc acaagcgcgg cgatcttgtc
     1141 agcctgaaac ctttccgctc taggaacgag agtctgatat ctcatcgtgc tcttgccatc
     1201 agtgctgtag aatcgcacca atgtaagcgt ttcatttgtt ataagaggtt cgatcttggc
     1261 ccgatgctcc tccgcaagct tggctaaatc atcctcactt atttcagaaa aagtcacggc
     1321 aatccgtatt ggtaacgagg gatcaaaaaa atgagtctgt aaaatagctg agcctgaaaa
     1381 aaatagggag agagcctgca gaacggatga ttttcctgaa ttattttcgc cgatgagaca
     1441 cccgaaacga gacataggca cgtctatatc gcgcagccct ttgtagttag cgatttgcag
     1501 gcgagtcaat tccatctcga ctcctttctg tgcgctgatt catcgcaagg aaatcccagg
     1561 cacgctgtta cgccaacatt ccatgctcaa gcgcgagcaa aggcaagcct tgaagttttg
     1621 agggagtctg ccccgccata tcagccctcg atggtccacc gcacagcaaa gagcggcttt
     1681 gatgccacta tttgcttgag ctggcgttcg agcccggcga tcagcttgtt gcgctgatcg
     1741 tcgatctggt cctgcgcgta gaagagttcg cagcgttttt tgtttcgcgc cgattcgagg
     1801 gctttcacct gtttttgagc ctccagcttg tcgcgcaggg aagcggccag cacggacagt
     1861 tttttggtct cccggatctc cttgtcgagt tccttgattt cccgctccaa gccctgtttc
     1921 aggtatttgg ttcctcgatc ccgcttgagt actaagtagg cattttgttc ttaggcgtgt
     1981 gctaaggcgg ccttgatgcg cgcgtcgcgg accggagtca gatcctgtag cggaatagtg
     2041 gccgcatgca ctgtagcaga aatgaggaga agttttcgac acaccgaatc gcgccattta
     2101 agatcttacc ggggatggta tcgtcctgcc atttgcgatc ttaccggaac atggaaggca
     2161 agacgatgtc aggagaacgg attttggatg cattgaacac gtatcggaag cgttatgcca
     2221 gagcggattg gccgggcagt tcggtgctgc tggacgaatt ttgagcccgg gccagctgcc
     2281 accgcaggag aagcggttca gctcacgcac cgggaatgaa aggcgatagt ccacccccgt
     2341 ccactcggag cgcgtagcgc aggcatcctg cctgcgctct tcagatgacg atccaaccaa
     2401 gttttcgtag cgcgtagcgc aggcatcttg cctgcgttct tctcttgcgg agcacaggcc
     2461 tctgtacctg ccctgtatcg gggtcaacgc gtggagctgg gatgggcggc cacagggggc
     2521 cgcccctaca atccggagcg cgaacgcctg gggctgccct ggtccattct ggttttcgta
     2581 gcgcgtaggg gcagacccct gtgtctgccc ttcttcagac gggtacacga gggctatccg
     2641 gatgggcggc cacagggggc cgcccctgca atccggatgg gcaaccgcgc aattcgactt
     2701 catgaccggt gcttgctaga ctacattctc ttcctgccca tgataacttc ctgaatacga
     2761 tgaacatgct cagccgaggg gattcatgaa tgcattcctg gccggattga atccggcaca
     2821 acgggaggcg gttacgcata ccgaagggcc ggtcctgatc ctggcgggcg ccggcagcgg
     2881 aaagacccgg gtcatcacgc agcgcatcgc gcatattctc gcgcggggca tcgcccaacc
     2941 cggagaaatc ctcgcggtga ccttcaccaa caaggccgcg ggcgaaatgc gggagcgggt
     3001 cgccgcgctg acgggggcac aagccgcgcg ggacctggtg atctcgacgt ttcattcctt
     3061 ttgccttcgc gtattgcggg cggaaatcac gcacctgggc taccggaagg atttcaccat
     3121 ttcgtcggag ggagacgccc gtaccatggt gcggcgcctg ctcgataacc tggacggcgt
     3181 gagggagaca tttgacccgg acagttttct cgagcagatc ggctgggtga aaaacagcgg
     3241 cttgtttgag gtgcctgaaa atcagccgga gggggatacg gacacggcca gaaaatacgc
     3301 cacgtggctg ccggcggtgt ttgaaaagta ccagtccgca ctccgcgccg ccaataccct
     3361 ggatttcgac gacctgctgc tgttgacgct gcgtctgtgg cgcgaacatc cccggatcct
     3421 cgcgcggtac cagaagcgcc tgcgttacgt gatggtggac gaataccaag acacaaatcc
     3481 gctccagtac gaattgcttc ggctgttggc gtcggctcac cgaaacctct gcgtggtggg
     3541 tgatgacgat cagtcgatct acggctggcg cggcgccgac atccagaaca tcctcagttt
     3601 cgagaaggac tatccggacg cgcggattgt cacgctcgaa cagaattacc ggtccacgca
     3661 gaccatcctg agcgcggcca acgccgtcat cgccaacaac cgggcgcgcc gcgaaaagcg
     3721 cctctggagc gagctgggag ccggccgccc cctcgactgg tttgtcaccg ccgacgaaga
     3781 acacgaagcg aagatgatcg tgtcgtggat acagcacatc cgagaccgga cccgcgcaaa
     3841 atacaccgat tttgcggtgc tctaccgctc aaatctgcag tcccggccga tcgagatcgc
     3901 catgcgccag gcgaagatcc cgtatgtcgt cgtgggcggg caggattttt tcgagcggac
     3961 cgaggtgaag gatatcatcg cctatttgcg cgctgtagtg aatccgcgcg acgaggcggc
     4021 ctttctgcgg attgccaaca tgccgcggcg cggcatcggc gacgccaccc tgcaccaggt
     4081 gcacgccctc tgtctcgccg agaatctccc tttcgcaaaa ggcctgtcgg aggccatgcg
     4141 ccggggtctg atccaaggac gggcggaggc aggcatccgc gaactgtcag ggctgctgcg
     4201 cggattcggg gagcgtttcc gccaggaagg cgtttcgttg acgcagcagg tgcaggaact
     4261 ggttgatcgc atcggttacc gggcggagct ggtgcgcacg agcaagagcg atctgcagct
     4321 cgagcagaaa tggtcaaacg tcgaagcagt cattgacgcg gtagcccaat acgagcagca
     4381 aagtccggtc cctcccacgc tgaacggttt tttggatgaa agcgccctga acaagaccga
     4441 agaccatcga gaggacggcc ggcagcaacg ctccggcgta accctgatga ccatccatag
     4501 cgccaaaggc ctcgaatttc cctttgtgct gattgcgggc attgaagaag ggctgctgcc
     4561 gcatgaaaaa tgcctccagg gtgctgcatt ggaagaggag cggcggctgt tctacgtggc
     4621 cctgacccgc gccaaacgcc atgccacgct gtttgaatcg tgcgtacgca gccggcgggg
     4681 acgcgaacgc ctgtccacga cgagccggtt catcaaggaa atccccgaaa cgctgctttg
     4741 ccgccacgtt cgagccgccc gcgagatgat cgctgagcgc acggcgccgg ctcaggcttc
     4801 ccagtcgcca aaacggcgcc gcagaagcaa gtagaggaaa aacggtccgc ctacaaacgc
     4861 cgtgatgatg cctatcggcg tttcaccggg aagcacgatg cgggcaaaga tgtcgcacag
     4921 acacagaaag gcaccgcccg ccagaaaaga caacggcatc agcaggcgat gacgcgaccc
     4981 cgtcagactg cgcaccgcat gggggacgat cagcccgaca aagccgatgg gtccgacttc
     5041 cgataccacc acggcggtga ggaacgaacc cagcagaaac gtggtagtct gaaggcggcg
     5101 cacgttcacc ccgcggctct gggcaatctc ggcgccgaaa cccaattgat cgaactgagc
     5161 cgcctgcagc agaagcacca tgctgcaggg cagcacgccc atggccagcg tggcgaccgg
     5221 ctcgtagccg ataatgtcca cgccgcccat cagccagcgt tccatcatgg ccagcatgcc
     5281 cggatccgac aaaaaccgca cgagcatcat ccccgcgttg gagagcatgc cgagcgtgat
     5341 gccggccagc aagagtaccg gcggcgagac gtgatgccga cgcacggcaa taaggtagat
     5401 caacgagaca tccagcagcg caaacacaaa ggcaaaggcc tgcacggccg aaaagccgcc
     5461 gagcgtgagg acgagcccgg cggagcgcat gacgaccgtc gcggtgtatg ctccaaacgc
     5521 tcccgcgctg gccacgccga gggtgtaggg cgtcgccagg ggattgcgca gaagggcttg
     5581 aaacacgcat ccggccagtg cgagtccgcc accggccaga agcgcggcca gggtgcgtgg
     5641 aaggcggtgt tgaagcagta tggccagttc cggcgcatcg gcgagcggcc gccccgcgcg
     5701 ccagtcgcgc caggccgcac cgaaatgaat cgattcgctg cccacgccga tgctggccag
     5761 cacgacggcc actgagacca ccatgaggcc cgccgtaatg aggctgccgc ggcaccgctt
     5821 catggcgccc cctcgcgcac agacgcgggc tcgggccaca ccagcggcgt tcccgtgatg
     5881 ggatgttccg tgaccacgat ggatgattcg taggcctcgc tcaggagatc gggcgtcaga
     5941 acctccggcg gcgtgccttg cgcgactatg cgggcccgcg acgacagcag cacgagccgg
     6001 tcgcagaatt gccccgcaac attgagatcg tgggttacca ccgcgacacc gtagccattt
     6061 cgggccaatc cgtgcagcaa cgagaagatt ccggcctggt gatggatgtc gagggccgcg
     6121 gttggctcat ccaggagcag caaatcgggt tcctgggcca gaatactcgc cagcagcacg
     6181 cgctgccgct cccctccgga caaggtgccg aaatcccggt ggcgcagatg ctccgtttcc
     6241 gtttcccgca tgcaacgcag cacgatttca cggtcacggc ccgatggcgt ccccaggggc
     6301 cccaaatgcg ggtaccgccc cagaatcacc acttccgccg ccgtcagcgt aaaggccgga
     6361 gcgacagtct gcgggagaaa cgcgaggata cgggcccgct cgcggttgtg cagggcgctc
     6421 agcgg
//
LOCUS       DAOPBQ010000173         6409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10664_length_6409_cov_3.894712, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000173 DAOPBQ010000000
VERSION     DAOPBQ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10664_length_6409_cov_3.894712"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKW60_07005"
     CDS             <1..238
                     /locus_tag="PKW60_07005"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99219.1"
                     /translation="YAGKLAYFMGISNARFRRTVVPGDQLVIESEMLQLRRNACRVRA
                     VARIEDDIAVEAEMSFGLTDAPSQDGQIHPRKSV"
     gene            complement(369..1502)
                     /locus_tag="PKW60_07010"
     CDS             complement(369..1502)
                     /locus_tag="PKW60_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99220.1"
                     /translation="MPDTKFVIVIAMIIFTIASIVTTYISLSISVLPEPSVTIPITEN
                     VTWDCAYLALMISVGIGLMLFALKVAIIDEQKRLNLTGVLGLLIIAFISVSFNMDVFY
                     RAANRDFYINFTSAQMKRVYADYLADVDAKLSARKTELLKQVAKQEGELESEIRGIRE
                     APEGYGPKAKSEDYKLTLLQKEAEVELRALDEVQSSRQHANELLVKSPQDLAGVEALQ
                     GELQAALKDLGAKAGIPLPEPYRAQNQLFVVFAKLFDFRNSSFMEICMSLLIMALALL
                     LDLGDIIGYSMVPDEPGGKRTRKHVDYDEYLGPEIVPAPLSANPMLARRETAVLDYQP
                     GDPGDDKEAFFARPRENYPLPEEEVEAAGQSRPRRPFRTRHRR"
     gene            complement(1705..2937)
                     /gene="ispG"
                     /locus_tag="PKW60_07015"
                     /gene_synonym="gcpE"
     CDS             complement(1705..2937)
                     /gene="ispG"
                     /locus_tag="PKW60_07015"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPJ99221.1"
                     /translation="MAVAKRRETVGVMVGNVQVGGGAPVVVQSMTNTDTADVDSTATQ
                     IMELADAGSELVRMTVNVPKAAEAAGEIVQRIRDAGYTTPIVGDFHYNGHKLLAQHPQ
                     CAQALDKYRINPGNTGKGSSRDANFETICKIAADHGKPVRIGVNIGSLNPELVLRKMQ
                     ENSDKSLGRSSDEVMNACMIISALESTELALEAGLRRDQIIISCKTSTPVHLIDLYRE
                     LSSKTEQPLHLGLTEAGMGSKGLLWSAAAVAPLLCEGIGDTLRVSLTPRPGSDRREEV
                     YACCDILQALELRAFAPSITSCPGCGRTTSTTFQELAQLTEAHIKERMPEWRKRYKGV
                     ETLKLAVMGCVVNGPGESKAANVGISLPGSGESPVCPVYVDGEKVASLKGTPEELASE
                     FRDIIDRYVETRFGPGTT"
     gene            complement(3070..3525)
                     /locus_tag="PKW60_07020"
     CDS             complement(3070..3525)
                     /locus_tag="PKW60_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99222.1"
                     /translation="MPCTDVTERIELVLDADDRLISYTLHKRSCGRTTGAEGLLEAVL
                     AGTAAAKIIRMTPDELLERFHPSGGSESSVALRHLFALQAACAVLSGAAPGGPRDLCA
                     AASIAWEEGMTRLTGLIQVNGAHEENRKCGCCQGCISKETALPCTPGHC"
     gene            complement(3543..3896)
                     /locus_tag="PKW60_07025"
     CDS             complement(3543..3896)
                     /locus_tag="PKW60_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HPJ99223.1"
                     /translation="MPGKLEDLALQDCLPCDGGVPALEGPELAVLMRKLDNRWRLVNE
                     HHLEREYTFNDYPDAVDFTNAVARLAQKQNHHPDLRLTYGKVVVTVWTHKANGLTLND
                     FVFAAKTEKLSQDSA"
     gene            complement(4122..>6409)
                     /locus_tag="PKW60_07030"
     CDS             complement(4122..>6409)
                     /locus_tag="PKW60_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPJ99224.1"
                     /translation="GENTGKGLLMWVDLGVPYHPDPRNAATFAGNRARSGRPAGAAAY
                     AALPAPALSGGGQARAALASDPVLLTGTNTFRFDVSNPERRRQALMMEMVFPDGSVHR
                     CVRHVFGPKERVAIAFDTRTTGTYEFKTRLVDADARTASAWEVTRLQFDGLPSDTRYL
                     NDVVFAETGRALDSWQASNPRGADTLRREVLANRGLLAQLADVEAERRPDVLATLRHD
                     AERIRALAAASAALAPSGSFHAWEFCPWAYFDPRESLPTAASQAPELTASLCIGEYES
                     LALNLTNLCERTLDIRVLLSNEQVPEDCQPLPLETCIELRRAVSVPAYERGMVSDALP
                     LLDQASAVSIASLESQQLWITVNAKGLAAGKYAAVLRLKSIEPDPTEIHVPLELTVHD
                     LALPRPRPLRFCMWSYDGGPMGTDRPEVLAELVEHGTTVFFGTCPGAECDADGNLTAP
                     VDFTAHDESVARLAPHGFLLFTSPQGYVKGAPFLSEPWRKAFVAYIRAWSAHMTELGV
                     DPALWALYPYDEPSAPYSDTTINLVEVAKLVREADPTVLIYTDPTSGTTAESLDMYKE
                     LIDIWCPAYELLTRFGPEFLSVAREYATELWYYDAPGRAKTLSTLSHYRRWIWYAWNQ
                     GFTGAGWWVFAMHGNADRWDGPNPTGNFYPTAYDGPHGVVTSRRWEVTREAIEDYEYL
                     CMLREAIREAEARGVDDSMLAGPRKLLSEMPPAMEETLRATGRRLILSPDGVPAYEEA
                     TRTLEDARKQIVEACLKLKSQ"
BASE COUNT         1145 a         1998 c         2011 g         1255 t
ORIGIN      
        1 atatgcgggc aagctggcct acttcatggg gatcagcaac gcgcgtttcc ggcgcacggt
       61 cgtgcccggc gaccagctgg tcattgaatc ggaaatgctt cagctccgcc gcaatgcctg
      121 ccgcgtgcgc gccgtggccc ggatcgaaga cgatatcgcg gtagaggccg aaatgtcgtt
      181 tggcctgact gatgccccct cccaggacgg gcagatccac ccgcgcaaat ccgtctgaag
      241 actccgccgg gctgactggc gagcgcaccg ctctgcacgg cgagtgcgct ccctttcaga
      301 atgcacccaa ccgcagacgc atttggggac tcgtgctgtg cggccgtcgg tcggggctcc
      361 cccctcttct aacggcggtg gcgcgtccga aaaggcctcc gggggcggct ctggccagcc
      421 gcttcgactt cttcctcggg aagcgggtaa ttctcccggg gccgggcgaa gaaagcctct
      481 ttgtcatcgc ctgggtcgcc gggctggtag tcgagcacgg cggtttcccg tctggccagc
      541 atggggttgg cgctgagggg cgccggcacg atctccgggc ccaagtactc gtcataatcc
      601 acatgcttcc gtgttcgctt accacccggc tcgtcgggca ccatgctgta gccgatgatg
      661 tcacccaagt cgagcaggag tgccagcgcc atgatcagca gcgacatgca gatttccatg
      721 aaactggaat tgcggaaatc gaagagcttc gcgaacacca cgaagagctg gttctgcgcc
      781 cggtacggct ccgggagggg gatgccggcc ttggcgccca gatctttcag ggccgcctgc
      841 agttcgccct gcaacgcctc cactcccgcc agatcctggg gcgatttcac gagcaattcg
      901 tttgcatgtt gccttgagga ctgcacttcg tccagtgccc gcagctcgac ttccgcttcc
      961 ttctgaagca gcgtcaattt gtagtcttcg cttttcgcct tggggccgta gccttcaggg
     1021 gcctcccgga ttccgcgaat ctcggattcg agttcaccct cttgtttggc cacctgtttg
     1081 aggagttcgg tctttcgcgc cgaaagtttg gcgtctacat ccgccaggta gtcggcatag
     1141 acccgtttca tctgcgcgct cgtgaagttg atgtagaagt cgcggttggc ggcgcggtaa
     1201 aacacatcca tattaaatga gacggagatg aacgcgatga tcaaaagccc caggacgccc
     1261 gtcaggttga gccgcttctg ctcgtcaata atcgccactt tcagcgcaaa cagcataaga
     1321 cctatgccca ccgagatcat aagcgcaaga taggcgcaat cccaggtcac gttttccgtg
     1381 atcgggatgg tgacgctcgg ttccggaagc acggaaatgc tcaagctgat ataggtcgtc
     1441 acgatggatg caatcgtaaa gatgatcatc gcgattacaa tgacgaattt ggtgtctggc
     1501 atgggtttct cctcgccgca tcttccgggc aggcactcat ctggccgcgg gacacaaccc
     1561 gcccgcctat gatccccgga cgcggtgcgc ggttccgttg tcgtgagctg cggcccctgt
     1621 ccacgtgcgc ccgccgcgct cacaaggaaa catcatatca gaaatgtcag aaaatcggga
     1681 attccgtgcc ggcgtccggc cgcatcaggt cgtgcccggc ccaaaccggg tctccacgta
     1741 ccggtcaatg atgtcgcgaa actcgctggc cagctcctcg ggcgtgcctt tcaggctggc
     1801 caccttctcc ccgtccacgt acaccgggca gaccgggctt tcgcctgagc cgggcaggct
     1861 gatcccgacg ttagcagctt tggattcgcc ggggccattg acgacgcagc ccatgacggc
     1921 gagtttgagg gtctccacgc ctttgtagcg tttacgccat tcgggcattc gctctttgat
     1981 gtgcgcctcg gtcagttgag ccaattcctg aaatgtggtg ctggtggtcc ggccgcagcc
     2041 cggacaggag gtgatgctcg gggcgaatgc ccgcagttcc aacgcctgca agatgtcgca
     2101 gcaggcatag acctcttcac ggcggtcgct gcccgggcgc ggagtcaggg agacgcggag
     2161 ggtgtcgccg atgccttcgc acaggagagg ggccacagcg gctgccgacc agagcagccc
     2221 cttggacccc atgcccgcct cagtgagccc gagatgcagc ggctgctccg ttttcgatga
     2281 caattcccgg tacaggtcaa taaggtgcac gggcgtggat gttttgcacg agatgatgat
     2341 ctggtcccgg cgcaggccgg cctccagggc gagctcggtg gactccaggg ccgagataat
     2401 catgcaggcg ttcatgacct catccgagga gcggcccagg ctcttgtcgc tgttttcctg
     2461 cattttgcgc agaacgagtt cgggattcag cgaaccgatg ttgacgccga tccggaccgg
     2521 tttgccgtgg tcagcggcga tcttgcagat ggtctcgaaa ttggcgtcgc ggcttgaccc
     2581 tttgcctgtg ttgccggggt tgatccggta cttatccagg gcctgggcgc attgcggatg
     2641 ctgggccagg agtttgtggc cattgtaatg gaaatcgccc acaatgggcg tcgtatagcc
     2701 ggcatcccgg atgcgctgta cgatttctcc ggccgcctcc gccgctttgg ggacattgac
     2761 cgtcatccgg accagttccg agcccgcatc ggccaattcc atgatttgtg tcgcggtgga
     2821 gtccacatcc gccgtgtcgg tattggtcat ggattgaacc acgaccggtg cgccgccccc
     2881 cacctggacg ttgccgacca tgaccccgac ggtctcgcgc cgttttgcca ctgccattgg
     2941 tgattgtcct cttgaaaatg ccagtaccgg ccctgggaaa gtgcctatcg ctttcaaccg
     3001 gatggaatgc gcaggttttc cgtgatcata gcgcatcgcc gtgccggatg cgaacgcgga
     3061 gatggccggt caacagtgac ccggggtaca gggcagggcg gtttccttcg aaatgcagcc
     3121 ctggcagcag ccgcatttcc ggttctcctc gtgcgcccca ttgacttgga tcagcccggt
     3181 cagacgggtc atcccttcct cccaggcaat actggccgcg gcgcagaggt cgcgaggacc
     3241 gccaggagca gccccactga gaacggcgca agccgcctga agcgcaaaaa ggtgtctcag
     3301 cgcgacggag ctttcgctcc cgccggacgg atgaaatcgc tccagcagct cgtccggggt
     3361 catgcggatg atcttcgccg cggccgtccc ggccagcacg gcctccagca gcccttctgc
     3421 gcctgttgtc cggccgcagc tgcgcttgtg aagggtatac gagatcagtc tgtcatcggc
     3481 atcgaggacg agttcgatac gttcggtcac atctgtacag ggcatggctc gcttcgcgcc
     3541 tgtcaggcgc tgtcttgaga cagtttttca gttttggcgg cgaacacgaa gtcgttgagt
     3601 gtcaggccgt tcgccttgtg ggtccacacc gtgaccacga ccttgccata ggtcagccgc
     3661 aggtccggat gatggttctg tttctgggcc agacgggcca cggcgttggt gaaatcgacc
     3721 gcgtctggat agtcattaaa cgtatattcc cgctcgagat ggtgctcatt gaccaggcgc
     3781 caccggttat cgagctttcg catcagcacc gccagctccg gtccttcgag ggccggcacg
     3841 ccgccgtcgc acggcagaca gtcctgcagc gccaagtcct ccaatttacc aggcatggat
     3901 gcctccttca ctctgtccgg aacagacagt ttctttcgta gacattcacc agggcgcgcg
     3961 gtattccccg gaacccgggc agggctgcaa cgataccacg gtttggagat cctgccggag
     4021 gcgaatgtgt ccaacgcgct tccgaatgaa tgcggaccga catctcaacg cctctctcga
     4081 aaatcctttt cactggacga gcctgccgaa tggcccgggg ctcactgact cttgagcttc
     4141 agacacgcct ccacgatttg ttttcgggcg tcctccagag tgcgggtggc ttcttcgtat
     4201 gcgggtacgc cgtccgggct caggatcaga cggcgccccg tggcccggag cgtttcctcc
     4261 atcgccggcg gcatttcgga caggagcttc cgcggcccgg cgagcatgct gtcgtcaacg
     4321 ccgcgggctt ctgcctcgcg gatcgcctcg cgcagcatgc aaaggtattc gtagtcctca
     4381 atggcctcgc gggtcacctc ccaccggcgg cttgtcacaa ccccgtgagg gccgtcatag
     4441 gccgtcggat aaaagttgcc ggtggggttg ggtccatccc agcggtctgc gttgccgtgc
     4501 atcgcgaaaa cccaccagcc ggcgccggtg aagccctggt tccaggcgta ccagatccac
     4561 cggcggtaat ggctcagcgt ggacaaggtt ttcgcgcggc cgggcgcatc gtaataccac
     4621 aactccgtgg cgtattcgcg ggccacggat aaaaactccg gcccgaaccg ggtcaacagc
     4681 tcataggccg ggcaccagat atcgatgagt tccttataca tgtccaggct ttccgccgta
     4741 gtgccgctgg tggggtcggt gtaaatcaga acggttggat cggcctcccg gacgagcttc
     4801 gcgacctcaa cgaggttgat cgtggtatcg gaatacgggg cggacggctc atcgtagggg
     4861 taaagggccc agagtgccgg atcgactccg agttcggtca tatgggccga ccaggcccgg
     4921 atataggcca cgaacgcctt gcgccagggt tccgacagaa acggcgcgcc tttgacatag
     4981 ccctgcggcg acgtaaacaa gaggaatcca tggggcgcca ggcgggccac ggattcgtcg
     5041 tgcgcagtaa aatcaacggg cgcggtcaga ttgccgtcgg cgtcgcactc ggcgcccggg
     5101 caggtcccga agaacacggt ggtgccatgt tcgaccagct ccgcaagcac ctccggccga
     5161 tcggtgccca tcgggccgcc gtcataggac cacatgcaga agcgcagcgg ccgcgggcgg
     5221 ggaagagcca ggtcatgtac ggtgagttcc aggggtacgt gaatttcggt cggatcgggc
     5281 tcgatgctct tgagccgcag taccgcggcg tacttgccgg ccgccagtcc tttggcgttg
     5341 accgtaatcc acagctgctg cgactcgagc gaggcgatgc tgacggcgga tgcctgatcc
     5401 agcagaggca gggcgtccga caccatacct cgctcgtatg ccggcaccga aaccgcgcgc
     5461 cggagttcaa tgcaggtctc cagcggcagc ggttggcagt cttcgggaac ctgctcattt
     5521 gacaggagca cccggatgtc gagcgtacgc tcgcagaggt tggtcagatt gagcgccagc
     5581 gactcgtact cgccgatgca cagggaagcc gtgagttccg gcgcctggga tgcggctgtg
     5641 ggaagcgact cgcgcggatc gaagtacgcc caggggcaga actcccacgc gtggaagctg
     5701 cccgaaggcg ccagcgcggc agaggcagct gcgagtgcgc ggatgcgttc ggcatcgtgg
     5761 cgcagcgtcg caagcacgtc gggtctgcgc tcggcttcta catcggccag ctgggcaagg
     5821 aggccccggt tcgccaggac ttcccggcgc aaggtgtccg caccccgcgg atttgaagcc
     5881 tgccaggaat caagagcgcg cccggtctct gcgaacacca cgtcgttaag atagcgcgta
     5941 tcggacggga gcccgtcaaa ctggagacga gtcacttccc aagcggacgc agtccgggca
     6001 tcggcatcca caaggcgggt cttaaactcg taggttcctg ttgtgcgtgt atcaaacgct
     6061 atggcgacgc gttccttcgg gccgaacaca tggcgtacac agcggtgaac ggagccgtcc
     6121 ggaaacacca tttccatcat gagcgcctgc cgccgccgct ccggattgct gacatcgaac
     6181 cgaaacgtgt ttgtcccggt cagcaggacc ggatcgctcg ccagcgcggc gcgggcctgt
     6241 cctccgcccg agagcgccgg cgcggggagc gcagcatacg cagccgctcc ggcagggcgc
     6301 ccggaacgcg cgcggtttcc ggcaaatgtg gctgcgtttc gtggatcggg atggtacgga
     6361 accccgaggt cgacccacat gagcaggcct ttgcccgtgt tctcgcccg
//
LOCUS       DAOPBQ010000174         6407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10665_length_6407_cov_3.501417, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000174 DAOPBQ010000000
VERSION     DAOPBQ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10665_length_6407_cov_3.501417"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="PKW60_07035"
     CDS             complement(<1..1534)
                     /locus_tag="PKW60_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99225.1"
                     /translation="MKERFGQYPGGAPWVQRFLDARMARAAASQRIIAQTLGIEIHPD
                     TGRLQGHGTLDIECSGAGLDVVSFILNPGLNSFSALCDGQKLPVSRWGGLVRAALPKR
                     LAPGECTRIAVSYSGDISATALTDAEIHPEEVWLPWTACWHPVDFGSFAEFDCAVTIR
                     GDLVLAGDAQETFPSVEGFRSYRIQPACKALGIPLLAGGYSSRAGTHGDLQCTVYFEP
                     GDAAIAEPLAASLPETEDALRALLGGHELPTYAILSRRYRTPRYLGLNIIALPRTPQQ
                     DESTALAATAAALAHKWWGQTVSPAWFPLFPDGGAWLLEGLAVHSGWSALRELKGKSA
                     LQEHLENQFPQPLEKPFRALGALELARSEPNAASRIGSQGAFLARWLESGAGKDAYWE
                     ACRNVLHTHRGNAITLDAFRHALEVASGRDLGELFRAWFDHAGLFDYAVDDVSAGDGA
                     IRVTISNHGDMPSLQPLTIGVVTARGVDLHAIDAGGNGGVFSFAVDSPVSRVTLDPYF
                     ECP"
     gene            complement(1652..2920)
                     /locus_tag="PKW60_07040"
     CDS             complement(1652..2920)
                     /locus_tag="PKW60_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99226.1"
                     /translation="MANVKAFDVTIVGGGMITCDLLLPSIYHLQRLGVVGEIHICALN
                     NTPLRALKENPQFAQAFPGQDFVAHPSLNEPADASFPELYKEVLAAMEPYNAVVVAMP
                     DQLHYPVVMEALNNNQHVLCVKPLVLKYAQAVEIEEVARQKGLFVGVEYHKRFDRRAL
                     IARGRYEQGHFGDFVMGEAKLIEPYLYRHSNFQNWFTCENTDPFVYIGCHYVDLTYFI
                     TGLKPAGVSVHGVKGTFPNGNTGYLWSQGRVVFENDAVLSVINGLGYPDEGAGPNEQG
                     LVLYCEGPGKTGLIQHNDQFRGVSHSYLSGLGPGGTAFNFVNPDFFQLGSWEGVGYKP
                     MGYGYDSVAAILGCIGRLRRETAGMPESSALTKRQEIISEIDEKRLIATPANSSINEL
                     VTEAARMSILSDGDFVKIVYDGFPRIEPKS"
     gene            complement(3009..4178)
                     /locus_tag="PKW60_07045"
     CDS             complement(3009..4178)
                     /locus_tag="PKW60_07045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPJ99227.1"
                     /translation="MRAWTEAKSWTRVPVLGAMLCAALAAPTVFAGEMDAPVLSAPSL
                     ESQPQPIPEIVAKIEDEVITGLELQMAMNTAMRAKAARDASNPNRDPNAPPEPFDRDD
                     AERVLESLIRGKAIYVLAKKAKTSVTDEEVDEGLAEAKKRLPEGEFEHLMEDRGITAQ
                     ELDAKMREQLTCQKFFDEKTKDVEVTDEDVEQVYQKLKEAGRMQKPETADVAHILILA
                     PREGTDEAIAEAKERIDAARDRVVNGKEDFGEVAKDVSEDRSSAPLGGLYENVPPNMM
                     VPEFDKLMFELPIGEVSEPFQTRYGWHIMKVSERDPARTLEFDEVKDGIRRNLEAQAK
                     QRAFGEFLETAIGDVKIEILLPRKDAAAPSPDSSAGIPAEGSKAGEEPELPDEPS"
     gene            complement(4287..5330)
                     /locus_tag="PKW60_07050"
     CDS             complement(4287..5330)
                     /locus_tag="PKW60_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99228.1"
                     /translation="MAEIGYGIIGCGNIAPMHAEAIQKVEGARLVAVSDVVEQNAKQL
                     AERFGATPYSDYRELLARDDIQVVNLCIPSGMRAEVGTECARAGKHIISEKPLEVNTE
                     RIDALLQAVDEAGVKLACIFQNRFADGAQRVKEAVEAGRFGKLVLGDAYIKWYRSDEY
                     YKSGSWRGTKSLDGGGCLMNQGIHQIDLLLWFFGPVKSVFARTSLVGHEGLEVEDLAC
                     AILEFENGSMGVIEGSTAIWPGHPARVEIHGDQGGAALEDGILRFWKFKEEGPDDESI
                     RAQMPVAAPGAGAADPMANVTCEGHRRQIEDFTDAIRHNRAPKIDGKEGRRSVALIEA
                     IYESSRTGRPVSL"
     gene            complement(5848..>6407)
                     /locus_tag="PKW60_07055"
     CDS             complement(5848..>6407)
                     /locus_tag="PKW60_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPJ99229.1"
                     /translation="GTTAPKTLRYLAEQPQAAHDVLEKLARMTTDYLMLQITAGADAV
                     QLFESAAPVLDAVAYREFALPYQQLIFDAIRGAAPTIAFARDIEAVELLDASGADILS
                     LPASISIAEARNLTSADRVMQGNLDNKLLVEGPLDAITRAAHAIIQAGDSRGHIFNLN
                     HGLLRQTPFEHVCHLVDTVRRWGDA"
BASE COUNT         1216 a         2021 c         1840 g         1330 t
ORIGIN      
        1 cggggcattc aaaataggga tcgagagtca cgcgggagac ggggctgtcc actgcaaacg
       61 aaaacactcc tccattcccc cccgcgtcaa ttgcatgcag atccacgccc cgggcggtca
      121 caactcctat ggtcagcggt tgcagggaag gcatgtcgcc gtggttggaa atagtgaccc
      181 ggattgctcc atcgcccgcg ctgacatcat ctactgcgta atcaaacagc ccggcatggt
      241 cgaaccaggc acgaaacagc tcccccagat cgcgcccgct tgcgacctcg agtgcgtgcc
      301 ggaatgcgtc cagggtgata gcgttgccgc ggtgcgtgtg caagacattt ctgcaggctt
      361 cccaatacgc gtcttttcct gcgccggact ccagccaccg ggcgagaaat gcgccctggc
      421 ttcctatgcg tgatgccgca ttgggctccg aacgggccaa ttccagtgcg cccagcgctc
      481 ggaagggctt ctccaggggt tgagggaact ggttttcgag atgctcctgc agggcgctct
      541 tcccctttaa ttcgcggagg gctgaccagc ccgagtgaac cgcaagacct tcaagaagcc
      601 acgcgccacc atcgggaaac agaggaaacc aggccggaga aaccgtctgg ccccaccatt
      661 tgtgtgcgag tgcggccgcc gtggcggcca gtgcggtgct ttcatcctgt tgcggcgtcc
      721 gagggagagc aatgatattg agcccaagat atcgcggcgt acggtagcgg cggctcagaa
      781 tggcgtacgt cggcagttcg tggccgccca gcagcgcgcg aagcgcatcc tctgtctccg
      841 gcaggctggc tgcgagcggt tccgcgatgg cggcgtcgcc gggttcgaaa tagaccgtgc
      901 actggaggtc cccatgggtg ccggctcgac tggaatatcc tccggccagc agtggaattc
      961 ccagcgcttt gcatgccggc tgaatgcggt agctgcggaa gccttcgacg gaaggaaatg
     1021 tctcctgtgc gtcgccggcc agtacgagat cgcccctgat cgtgacggcg cagtcaaatt
     1081 cagcgaaact gccgaagtcc acgggatgcc aacacgctgt ccagggaagc cagacttcct
     1141 ccggatggat ttctgcgtca gtcagcgcag tcgccgagat gtctcctgaa tacgagactg
     1201 cgattcgggt gcactccccc ggcgccagcc gcttcggaag ggcggcgcgg accaggcctc
     1261 cccagcggga gaccggaagc ttttggccgt cgcacagtgc agagaacgag ttcaggccgg
     1321 ggttgaggat aaatgagacc acgtcgagcc cggcgccgct gcattcgatg tcgagcgtgc
     1381 catggccttg caggcgcccc gtgtcgggat ggatctcaat ccccagggtc tgggcaatga
     1441 tgcgctggga tgcggcggcc cgggccatgc gcgcatccag aaagcgctgc acccaggggg
     1501 cgccgccggg atactggccg aaccgttctt tcacggcacg atacgagaga agcgcccccg
     1561 ccaacaaaac caacagcccc gcaagaaagg ctgtcctcgc cgttcgccgc ctctttattg
     1621 agtcacgacc gctcaatgtg ctccgttcga ttcagctttt cggctcaata cgggggaacc
     1681 catcgtagac gatcttcacg aagtctccgt cgctgagaat agacattcgg gctgcttccg
     1741 taacgagctc attaatgctg ctgttcgcgg gggtggcgat gagccgtttt tcatcaattt
     1801 cggaaatgat ttcctgacgt ttcgtcaggg cgctgctctc cggcattcca gccgtttccc
     1861 ggcgcaggcg gcctatgcac cccagaatcg ccgctacaga atcgtatccg tagcccatgg
     1921 gcttatatcc cacaccttcc cacgagccca gctggaaaaa gtctggattg acgaagttga
     1981 aggccgttcc gcccggcccc aggccactca ggtaggaatg gctcacgccg cgaaactggt
     2041 cgttgtgctg aatcagcccc gtcttccccg ggccttcgca gtacaggacc agtccctgct
     2101 cattcggtcc cgcgccttcg tcggggtaac ccaatccgtt gatgacggac agcaccgcat
     2161 cgttttcgaa tacgacccgg ccctgagacc agagataccc cgtattgccg ttggggaatg
     2221 tccccttgac gccatgaaca gataccccgg ccggtttcag tcccgtgatg aaataggtca
     2281 ggtcaacgta atggcatcca atgtacacga aggggtctgt gttttcgcag gtgaaccagt
     2341 tttggaaatt ggagtgccgg tagaggtacg gctcgatcag tttggcctcg cccataacga
     2401 aatcgccgaa atggccctgt tcatagcgtc cacgggcaat gagcgcgcgc cggtcgaagc
     2461 gcttgtggta ttccactccg acgaaaaggc ctttctgccg cgccacttcc tcgatctcaa
     2521 ccgcctgggc gtacttgagc accagcggct tgacacacag cacgtgctgg ttgttgttca
     2581 acgcctccat caccaccggg taatgcagct ggtcgggcat ggccaccact accgcattat
     2641 acggctccat cgcggccagc acctctttgt agagctcagg gaaactggcg tccgcaggct
     2701 cgttcaggga ggggtgtgca acaaaatcct gcccgggaaa ggcctgggca aactgcggat
     2761 tctctttcaa cgcgcgcaag ggtgtgttgt ttagcgcgca aatgtggatc tcgcccacca
     2821 cccccagccg ctgcagatgg tagatggatg gaagaagcaa atcgcaggtg atcatgcctc
     2881 cgccaacaat ggtcacatca aaagccttca cattcgccat gtatatccct ttccatgatc
     2941 gtgtgcaaag cacttttgcg cgcgccgtgg cgtactgcgc gcttcatgtc cagcccggcg
     3001 cgaagccgtc acgacggctc atcgggaagc tcgggttcct cgcctgcttt gctcccctca
     3061 gctggaattc cggcagaact gtcaggtgac ggtgctgcgg cgtctttcct ggggagcaag
     3121 atctcgatct tgacgtcacc gatggccgtt tcgagaaatt cgccaaaagc gcgctgcttt
     3181 gcctgtgctt ccaggttcct ccggatgccg tctttcactt catcaaattc cagggtccgg
     3241 gccggatcgc gctcactgac tttcatgata tgccacccgt aacgggtctg aaacggctca
     3301 ctgacctcgc ctatgggaag ctcgaacatg agtttgtcga attccggcac catcatgttg
     3361 ggcggtacat tctcgtacag accgccaaga ggcgcggacg atcgatcttc ggacacatct
     3421 ttggcgactt cgccaaagtc ttccttgcca ttaacgaccc ggtcccgcgc cgcatcgata
     3481 cgctcctttg cctccgcgat agcttcatcc gtaccctcgc gaggcgccag gatcaaaatg
     3541 tgagccacat cggcggtttc aggtttctgc atgcgccccg cttctttcag tttctgatac
     3601 acctgctcta cgtcttcgtc cgtaacctca acgtctttgg tcttctcatc gaagaatttc
     3661 tggcaggtga gctgctcccg catctttgca tccagctctt gcgccgtaat gccccggtct
     3721 tccatcaaat gttcaaactc gccttccggc agcctctttt tggcttccgc aagcccctcg
     3781 tccacttctt catccgtgac ggaggtcttg gctttctttg ccagcacata aatggccttg
     3841 ccgcggataa gggactccag cacccgttct gcgtcatcgc gatcaaaggg ctctgggggc
     3901 gcgttcggat cccggttggg gttggacgcg tcccgggcgg ctttcgcgcg catggcggtg
     3961 ttcatcgcca tctgaagctc cagccctgta atcacttcat cttcaatttt cgccacaatt
     4021 tccgggatgg gctggggttg gctctccaga ctgggcgcgc tcaacaccgg tgcgtccatc
     4081 tcacccgcga ataccgtcgg cgccgccagc gccgcgcaca gcatcgcgcc cagtaccggc
     4141 accctggtcc agctcttcgc ttcagtccat gcacgcattg ctctgtctcc tggttcctgt
     4201 ggaaccctgg cgataccgaa agcggttcga tgggcggccg gttgtcgata tctggccgtt
     4261 ttctaatgac ggatacggac tcttggttac aaactgacgg ggcggccggt acgcgacgat
     4321 tcgtaaatgg cttcgataag cgccacggat cgccggcctt ccttcccgtc gattttcggc
     4381 gcacgattat gcctgatcgc gtccgtaaaa tcctcaatct gccggcggtg accctcacac
     4441 gtcacgttgg ccatcggatc tgcagctcct gcgccggggg cagccacggg catctgagcg
     4501 cggatggact catcgtccgg gccttcctcc ttgaatttcc agaagcgcag gatgccgtct
     4561 tccagcgcgg cgccgccctg gtcgccgtgt atttccacac gcgcaggatg gcccggccat
     4621 atggcggtgc ttccttcgat gacccccatg gaaccgttct cgaactccag gatggcacaa
     4681 gccaggtctt ccacctccag cccttcgtgg cccaccaggc tggtgcgggc gaaaacgctc
     4741 ttgaccgggc caaaaaacca caacaacaga tcgatctgat ggatgccctg attcatcagg
     4801 cagccgccgc catccaggct cttcgtgccc cgccaggagc cgcttttgta gtattcatcc
     4861 gaacggtacc atttgatata ggcatcgccc agcacgagct tgccaaagcg tccggcctcg
     4921 accgcctctt tgacccgctg cgcgccatcc gcaaagcggt tctggaagat gcacgcgagt
     4981 tttacgccag cctcatcgac ggcctgaagc agagcgtcaa tccgttcggt attcacctcg
     5041 agaggctttt cggagatgat gtgtttcccg gcgcgggcac attcggtgcc gacctcggcc
     5101 cgcatgccgc tgggaatgca gagatttacc acctggatgt catcccgtgc caaaagctcg
     5161 cggtagtcgc tgtacggggt ggcgccgaag cgctcggcca actgcttcgc attctgctcg
     5221 accacatccg ataccgcgac cagccgcgcc ccttcaactt tctgaatggc ctccgcgtgc
     5281 atgggtgcaa tgtttccaca accgataatg ccgtacccga tttccgccat ggttttcatc
     5341 cctgtttcgc tgttggtttt cgtcagctcc cacaaccgta atccggatcg cgccgtgttg
     5401 tgccgccggc tcaggtgcgg cattggcgcg cgcgcacggc gtgacgccgt ggtgctggtc
     5461 gcgctcgaat cgccggcatc cgcaacccgg gagacgccat cataacagat gcggagcccc
     5521 ggatgcatgg aggcatagcg cgacgaccgc cagcgccagc gcgggacaca aaaatccgga
     5581 gcccgagaaa ttttagatgc ggcgcgcgtg tctcccgctg gcgggggtga atcggcggca
     5641 gccggttcgg gggtggattt tgagggtaaa tttagagcct ggttcgcgct tcccacagcc
     5701 cagcagcagc aatgccgcac tggcccactt aaacacagaa taaaccctcg gcagaaggat
     5761 cagcgccacc tcctcaagcc tcgacttcac gcgcgcatca accgcgacgc cttgcccaaa
     5821 cagcaccctt cccttttaac tcagatctca ggcgtctccc cagcgcctga cggtgtccac
     5881 gagatggcac acgtgctcaa agggtgtctg ccgcaggagt ccgtgattca ggttaaaaat
     5941 gtggccacgg ctgtccccgg cttgaatgat ggcgtgcgcg gcgcgggtga tggcatcgag
     6001 agggccctca acgagaagct tattgtcgag attgccttgc attacccggt cggcgcttgt
     6061 caggttccgg gcctctgcga tcgaaattga ggcgggcaga ctcaaaatat ctgctccaga
     6121 agcatccagc agttctaccg cctcgatgtc gcgcgcaaac gcgatggtgg gtgcggcgcc
     6181 gcggatggca tcgaagatca actgctggta gggcaatgcg aactcacggt aagccacggc
     6241 atcaaggacg ggggcagccg attcaaagag ctgcacggca tccgcaccgg ccgtaatctg
     6301 cagcatcagg tagtcagtcg tcattcgggc cagcttttcg agcacgtcgt gggctgcctg
     6361 aggctgttcc gccaggtaac gcagggtctt cggcgcagta gtcccga
//
LOCUS       DAOPBQ010000175         6395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10694_length_6395_cov_3.209306, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000175 DAOPBQ010000000
VERSION     DAOPBQ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10694_length_6395_cov_3.209306"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PKW60_07060"
     CDS             complement(<1..244)
                     /locus_tag="PKW60_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPJ99230.1"
                     /translation="MALTVVALGCSGEDDSGSAGGALSSSRRDGTTSTSSTTVAPPAP
                     RSTVVIAHRGASAYAPEHTFASYDLALEQGADYIEQD"
     gene            complement(273..1364)
                     /locus_tag="PKW60_07065"
     CDS             complement(273..1364)
                     /locus_tag="PKW60_07065"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPJ99231.1"
                     /translation="MPRWFLPIAAIALAMHFSALAVEEKGDDPALAAPPIIMNPGPEY
                     ADSTRLFQGIPGIERAPNGRLWATWYAGGDNEGPLNYCALATSGDDGKTWSSVQIAID
                     PPGDVRAFDPCLWHDPSGRLWWFWAQGFSLWDGRAGVWAIATDNSGDASPVWSEPRRL
                     CDGIMMNKPTVTRSGEWLLPVAIWEFPARVMKEEYAHDITAGTGSNVWISRDQGATFE
                     LLGRPDVEGRACDEHMIIERNDGSLWVLVRTRTGLGEAISTDGGRTWTEHIVAETVTQ
                     INRSLDIDDFAGAEPLVAAFRSMLSAVGLTVNDEVAAVADEAADLCRQRDDARAAKEW
                     GRADALRGELEAMGYVVEDTATGTQVRRA"
     gene            complement(1425..2681)
                     /locus_tag="PKW60_07070"
     CDS             complement(1425..2681)
                     /locus_tag="PKW60_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPJ99232.1"
                     /translation="MTMRSGEKLAVHGGNPVRKEPMPPRHLFGEEEKQAALALFDKSI
                     ETGEAFGYNGPEEEAYCNRFAAFLGGGYADAVNSGTSAIYVALRALEIEPFTEVIVPS
                     VSDMGGVMPVPLMNCVPVPADCAPGTYNMGPEQVEQAISERTSAIVVAHIAGIPVDME
                     PIMQLARARGIKVVEDCAQAHGARYQGEYVGTFGDVAAFSTMSGKHHATAAQGGVVYT
                     RSQSLYWKSRQCSDRGKPFGLEGIESNVTCSLNLNLNDLSAAVGLVQLEKLPGMLDRI
                     RRVARQVIERCAPLQAVSIDQGPPDTENAFWFMVCRLDPAKVTCGLDEFVGALRAEGL
                     PFSAHYTTPFTQHAWFKNRAVFGSSGYPWMCPLYTGDPNRRYELPNWKANNDSLFLIK
                     IHEGMAAREAEDIANALAKVEAAFLK"
     gene            complement(2728..3714)
                     /locus_tag="PKW60_07075"
     CDS             complement(2728..3714)
                     /locus_tag="PKW60_07075"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HPJ99233.1"
                     /translation="MAGVEPQAIHRNAIVIDGANASKFDETLFHQMLMGGVTAANVMV
                     SVFDNFKETILKTCAWKRLYTQLDALIMPIHRADDFAAAKEYGKLGIILGLQNTTAIE
                     EDLELIPVLKGMGIRIMQLTYNERNYVADGCFERVDAGLSRFGLEVLKRLNEHHILVD
                     LSHAGPRSTVEATERTEAPVAVMHSSPKMLCDYPRNKTDEQIKLLAQKGGVFGCSFWP
                     YGLKKGVESTVSDYVDTIDHVVDLVGINYTAIASDFCMNRTKAELQRFQSGRTKPWRT
                     VDLDWPVVMPEGLREATDYLNVTSELARRGYSERDITKILGGNFIRLFEQVW"
     gene            3990..>6395
                     /locus_tag="PKW60_07080"
     CDS             3990..>6395
                     /locus_tag="PKW60_07080"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HPJ99234.1"
                     /translation="MVSVVLVLLLAGAQLQLTPVPQEVVFDGKNVFRLSPADPIVMSD
                     AQTAQRDTAVALIRSATGFELPLVTADLQPRGARGIYLGEPGAREPQATRKPGSIMSI
                     PSREPSTPPQGYSLRVTKDWIAVTGADPAGTFHGVQTLCQLINERGEIPCVTINDAPE
                     LAWRGAWVREALSTDQLDAFAALKCNLIAFESDDFLSGSPERLARWVDTFAEARRRHI
                     EPVPVIRTLKNIAPLLQRHPQAAIARTVTEKVRLEEDNWALLSHPNVLETPETPITVS
                     QGAVTYRPVRDFAIDRGDFAFPYKPASAPWLIRRNIGGAIPDGRIVTVTYTYVAPDTR
                     DGLPLSEAAERAWLDMLEPVMRDLRPRFILANHNWPGEIARDPRAAAFSGNAGAMLAD
                     SVRNLDQLIRRMAPDAQLLVVSDGFQGTAETMRAVAAALPRSAACVIQAPETPTALAP
                     LLGPWLGAGLDVFGMPQPGVQGAYAWSGLLSGKERRVRGLLVPLEAETVPTAAFKVAM
                     QSSWSTGTPHSPWPETLNAYFNADLWEPDYAEVLSAVADHIDRNTLAGVTPEETHRAY
                     LAFSKNMGAQLPASDPQVKLVSSLMASMTEYLALERDFTRDQQGGSIRKLVPLIERQT
                     ALDPRADEERAQRILGTVRGKGLFVPSSILFGVYLLPYREMKLLAGHRPLEIVAQPEY
                     TDTEHTAQATYDFLAEPGPICRVDFDTVGAAAVTLDRSRDGKSFEPVQQWTSRERGGF
                     RAPAILDTPVHTRYLRVTVDAPAEQAVLRNPRVFALKGPVVGVCGRSDVAPVLDGAFK
                     EKCW"
BASE COUNT         1128 a         2066 c         1963 g         1238 t
ORIGIN      
        1 ggtcctgctc gatgtagtcg gcgccctgct cgagggccag gtcgtaggac gcgaacgtgt
       61 gctcgggcgc gtaggccgac gcgccccggt gggcgatcac caccgtggac cgtggcgccg
      121 gtggtgccac ggtcgtgctc gatgtcgacg tcgtgccgtc gcgtcgactc gacgacagtg
      181 cgccgccggc actccccgag tcgtcctcgc ccgagcaacc gagcgcaacg acggtgagcg
      241 ccatgaccac cgccgtcaga cgccgcatcg gatcaggccc gccggacctg ggtgccggtt
      301 gcggtgtcct cgacgacgta ccccatggcc tcgagttcac cacggagcgc gtcggcgcga
      361 ccccactcct tggcagcgcg ggcgtcgtca cgctggcggc acaggtcggc ggcctcgtcg
      421 gcgacggccg caacctcgtc gttcaccgtg aggccgacgg cgctcaacat cgatcggaac
      481 gccgcgacca gcggctccgc accggcgaag tcgtcgatgt cgagggaccg gttgatctgg
      541 gtgacggtct ccgccacgat gtgctccgtc catgtcctgc cgccgtccgt ggagatagcc
      601 tctccgaggc cggtccgggt gcgcacaagc acccagagcg agccgtcgtt ccgctcgata
      661 atcatgtgtt cgtcgcaggc gcggccttcc acgtccggcc ggcccaggag ttcgaaggta
      721 gcgccctgat cgcgcgagat ccacacgttt gagcccgtgc ccgcggtgat atcgtgtgca
      781 tactcctctt tcatcaccct ggcggggaat tcccagatgg ccacgggcag aagccattcg
      841 cccgagcggg tgaccgtcgg tttattcatc atgatgccgt cacagagccg gcggggctcc
      901 gaccagacag gcgaggcgtc tcccgaattg tccgtggcga tggcccaaac gccggcgcgg
      961 ccgtcccaga ggctgaatcc ctgagcccag aaccaccaca acctcccgga cgggtcgtgc
     1021 cagaggcagg ggtcaaacgc gcggacgtct cccgggggat cgatggcgat ctgtacgctg
     1081 gaccatgttt tcccatcatc gccgctggtt gcgagtgcgc agtaattgag cgggccttcg
     1141 ttatcgcctc ctgcgtacca ggtggcccag agtcggccgt ttggggcgcg ttcgataccg
     1201 gggattccct gaaacagccg tgtactgtcg gcatattcag ggccgggatt catgatgatg
     1261 ggcggtgcgg ccagggctgg atcgtcccct ttttcctcca ccgcgagggc tgaaaaatgc
     1321 attgccaggg cgattgctgc aattggaaga aaccatcggg gcattgaatt catctcctca
     1381 aaaagagaac gcccgcgagc atgctgcggg cgttgtgccg ggccttattt caagaacgcc
     1441 gcttccacct ttgccagcgc attggcgata tcttccgcct cacgggctgc catgccttca
     1501 tggatcttga tgaggaacag cgaatcgttg ttggctttcc aattgggcaa ctcgtacctc
     1561 cggtttggat cgcccgtgta gagcgggcac atccacggat agccgctgct cccgaagacg
     1621 gcccggtttt taaaccacgc atgctgcgtg aagggtgtcg tgtaatgggc gctgaacggc
     1681 aggccttctg cccgcaacgc gccaacgaac tcatccaggc cgcaggtcac tttggcgggg
     1741 tcgaggcggc aaaccatgaa ccagaaggcg ttttccgtgt ccggggggcc ctggtcgatc
     1801 gagacggctt gcagcggcgc gcagcgttcg atcacctgcc gcgccacccg gcggatccgg
     1861 tcaagcatgc ccggcagctt ctcgagctgc accaggccca cggccgcgct cagatcgttg
     1921 aggttcaggt tcagtgaaca ggtgacgttg ctctcaatcc cttcaaggcc gaacggtttg
     1981 ccgcgatctg agcattgccg cgacttccag taaaggctct ggcttcgggt gtagaccacg
     2041 cctccctgcg ccgccgtggc atggtgttta cccgacatcg tcgaaaaggc cgcgacgtct
     2101 ccgaaagtcc ccacatactc cccctggtag cgtgccccgt gggcctgtgc gcaatcctcg
     2161 accactttga tgccccgggc gcgtgcaagc tgcatgatcg gttccatgtc gaccgggatc
     2221 cccgcgatat gggccacgac gatcgcgctt gtccgctcgc tgatcgcctg ctccacctgc
     2281 tccgggccca tgttataggt tccgggcgcg cagtccgcag gcacggggac acagttcatc
     2341 agagggaccg gcatgacgcc gcccatatcg gaaaccgacg ggacaatcac ctcggtgaac
     2401 ggctcaattt ccagcgcgcg aagggccaca tagattgcgg aggtgcccga attcacggcg
     2461 tcggcataac caccgcccag gaacgcggca aagcgattgc aatacgcttc ttcctcgggc
     2521 ccgttgtagc cgaacgcttc cccggtctcg atgctcttgt caaacagcgc gagagccgcc
     2581 tgcttttctt cttccccgaa cagatggcgc ggcggcatcg gttctttgcg gacggggttc
     2641 cccccgtgga cagccaattt ctctcccgat cgcatggtca tgctcgatag cctccccgga
     2701 ttgcagtttt gtctgatgat agcagcctta ccagacttgc tcaaagaggc ggatgaagtt
     2761 gccgcccaga atctttgtga tgtcccgctc gctgtatccg cgccgggcca gctccgaggt
     2821 gacgttgaga taatccgtgg cctcgcgcag gccttcgggc atgacgacgg gccagtccaa
     2881 atcaaccgtc cgccagggtt ttgtacgccc gctctggaat ctctgtagct ccgcttttgt
     2941 ccggttcata cagaaatcgc ttgcaatggc cgtataattg atgcccacca ggtcgaccac
     3001 gtggtcgatc gtgtccacat agtcggaaac ggtcgattcc acgccctttt tcaagccata
     3061 gggccagaac gagcagccga acacgccgcc cttctgagcg agcagcttaa tctgttcgtc
     3121 ggttttgttg cgtggatagt cgcacagcat tttcgggctc gaatgcatga cagccacggg
     3181 cgcctccgtg cgttccgtgg cctcgacggt gcttcgggga ccggcgtgcg agaggtcgac
     3241 caggatgtga tgctcgttca ggcgtttgag cacttcaagt ccgaatcgcg acagccccgc
     3301 atccacgcgt tcaaaacagc cgtcagcaac gtaattgcgt tcgttgtagg tcaactgcat
     3361 gatccggatg cccatccctt tcagcacggg gatcagctcc aaatcttctt caatggccgt
     3421 tgtattttga agcccgagaa tgattcccag ttttccatac tctttcgcgg ccgcgaagtc
     3481 atccgcccgg tggatgggca tgatcaacgc atcgagctgg gtatacagcc gcttccatgc
     3541 gcaggttttg aggatggttt ccttgaaatt gtcgaacacc gagaccatca cgttggccgc
     3601 ggtcactccg cccatcagca tctggtgaaa cagggtctca tcgaatttgc tcgcgttggc
     3661 cccgtcaatg acgatggcat ttcgatggat cgcctgaggt tccacacccg ccatggcatt
     3721 tttcctccaa cgcgcgttga gccctgattt catggcgtgg ccgcatctcg cggccgcgtc
     3781 tgccaccggt gtttccgtca cgcaaggata tcacatgttc agaaccggct gagaatcccg
     3841 cggaaggggc gcggcgcgtg aggatgcggc tccgggagca gagagccggt gccggccgcc
     3901 ggcgctccgc gtcacaatta cacccaccct gccatccttg gtatactggc agcggtgtag
     3961 acaaatcccg ctcttccccg gagggaacca tggtgtccgt agtgcttgtt cttctgctcg
     4021 ccggcgcgca actgcaattg actccggtgc cgcaggaggt ggtttttgat ggcaagaacg
     4081 tgttccggct ttcaccagcc gaccccatcg tgatgtccga tgcccagacg gcccagcgcg
     4141 atacggcagt cgcactgatt cgcagcgcga ccggatttga gctgccgctg gtgaccgccg
     4201 acctccagcc gcggggcgct cgaggcattt acttggggga accaggcgcg cgcgagccgc
     4261 aggccacccg caaacccggc agcatcatga gcattccaag ccgagaacct tccactccgc
     4321 cccagggcta ttctctgcgg gttacaaaag attggatcgc cgtcaccggc gccgacccgg
     4381 cgggcacatt ccacggcgta cagacgctgt gccagctgat caacgaacgg ggggagattc
     4441 cctgtgtcac catcaatgac gcgcccgagc tggcgtggcg tggcgcatgg gtgcgcgaag
     4501 cgctttccac ggaccagctc gatgcgtttg ccgcattgaa atgcaatctc attgcattcg
     4561 agagcgatga ttttttgagc gggtcacctg aacggctcgc gaggtgggtt gatacctttg
     4621 ctgaggcgcg ccggcgtcat atcgagcccg ttccggtgat tcgcacactc aaaaacatcg
     4681 cgccgctgct gcagcgccat ccccaggcag ccattgcgcg gacggtgacg gagaaagtcc
     4741 ggctcgagga agacaactgg gcgctcttgt cgcaccccaa tgtgctcgag acgccggaaa
     4801 cgcccatcac ggtctcgcag ggagctgtga cgtaccggcc ggttcgggat ttcgccatcg
     4861 accggggcga tttcgcattt ccgtacaagc cggccagcgc gccgtggctg attcgccgca
     4921 acatcggcgg agccattccc gacggcagga tagtgaccgt gacctacacg tatgtcgctc
     4981 ccgacacccg tgacggcctg ccgctgtcag aggctgccga acgggcctgg ctcgatatgc
     5041 tggaacctgt gatgcgggac ttgcggcccc ggttcatcct ggcaaatcac aactggccgg
     5101 gagagatcgc ccgcgatccc cgtgccgccg cgttttccgg gaatgccgga gccatgctgg
     5161 ccgattccgt gcgcaatcta gaccagctga tacgccgaat ggctccggat gcacaattgc
     5221 tcgtggtatc cgacgggttt cagggcacgg cggaaaccat gcgcgcagtt gccgcggcgc
     5281 tgccacgctc cgcggcctgt gttatccagg cgcccgaaac gcccacggcc ctggcgccgc
     5341 tcctcgggcc gtggctggga gccggcctgg acgtgtttgg gatgcctcaa ccgggagttc
     5401 agggcgccta cgcctggagc ggcttgctct ccggcaaaga acggcgcgtc cgcgggctgt
     5461 tggtcccact cgaagcggag accgtgccca cggccgcgtt caaagtcgcc atgcagagca
     5521 gctggtccac cggcacaccg cactctccct ggcccgaaac cctgaatgcc tacttcaacg
     5581 cggacctctg ggagccggac tacgctgaag tcctgtcggc cgtggccgat catatcgatc
     5641 gcaacaccct cgccggcgtg acgcccgagg aaacacaccg cgcgtatctc gcgttcagca
     5701 aaaatatggg ggcgcagctt ccagccagcg atccgcaggt taagctggtt tcgagcctca
     5761 tggcctccat gactgagtat cttgcgctgg agcgcgattt tacccgggat caacagggcg
     5821 ggagcatccg gaagctcgtg ccgttgattg aacgccagac cgcccttgat ccacgcgccg
     5881 acgaggaacg tgcgcagcgg attctcggca cggtccgggg gaaaggcctg ttcgtgccgt
     5941 cgtcgatcct gttcggcgtc tatctgctgc cttatcgtga gatgaagctg ctcgcagggc
     6001 atcggcctct cgagatcgtg gcacagccgg aatataccga taccgagcac accgcgcagg
     6061 ccacctacga tttcctggcc gaacccggcc ccatctgccg tgtcgatttt gacaccgtag
     6121 gcgccgctgc ggtcaccctg gatcgcagcc gcgacggcaa atcgtttgaa ccggtgcagc
     6181 aatggacctc gcgcgaacgg ggcgggttcc gtgcgccggc catcctggac acgccggtcc
     6241 acacccggta tctgcgtgtc acggtggacg caccggccga acaggccgta ttgcgcaatc
     6301 cgcgcgtatt cgccctgaaa ggtccggtcg tcggagtctg cgggcgctcg gacgtcgcgc
     6361 cggtgcttga tggagccttc aaggagaaat gctgg
//
LOCUS       DAOPBQ010000176         6339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10814_length_6339_cov_3.925843, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000176 DAOPBQ010000000
VERSION     DAOPBQ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10814_length_6339_cov_3.925843"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PKW60_07085"
     CDS             <1..906
                     /locus_tag="PKW60_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPJ99235.1"
                     /translation="YLSSESRWFEANLQAVERFPDALFLPGFWAEYGMCTEPSAFGAK
                     CIFPDNEFPFAERACESIEQAATLPKPDPRTDGLAPFVLKRLQHLEPRIVNAGHAIRF
                     AVARGPLNIAGFLLGNTELLLALKMDPDASHSLLNTLTSYLEDWLKLQAAAFPSIDGV
                     LMLDDLVGFLGPDDFQEFAQPYLKRAFEAIDARVRFFHNDAEGLICAPFLAGTGINLF
                     NFSHNHPLQAMREAAGEQVALLGNIPPRDVLAQGTPDQVRQSVRELLESITDTRGLIL
                     SCGGGMPPGVPAENIDAFLEAAADG"
     gene            992..1351
                     /locus_tag="PKW60_07090"
     CDS             992..1351
                     /locus_tag="PKW60_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPJ99236.1"
                     /translation="MYQSMPGSCLRFAPGDGIRYPQGMHELSFAQQILETIQRETAGY
                     EGARVTRVCLRADEYLALEPASLRFCLESIAVDTIMEGAVIEIEEEPLPPGAERPLFG
                     AGLVIEEIELDEQNDQT"
     gene            1329..1985
                     /gene="hypB"
                     /locus_tag="PKW60_07095"
     CDS             1329..1985
                     /gene="hypB"
                     /locus_tag="PKW60_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HPJ99237.1"
                     /translation="MSKTIKLEQKVQAKGHEIAAELRAMFRKKGIFVVNLIGSPGAGK
                     TSLLEALAPQLKGRAAVIEGDLATDNDKRRIEQAGLRAYQINTVGACHLDAAMVRDAL
                     NEFSLDGINLLFIENVGNLVCPASFEIGEDIKVAIVSTTEGEDKPAKYPKAMKVASAM
                     VITKVDLLPYVSCDVDRMERDVRELVPDMPVFRTAATTGEGTGEFLQWIEAQAAARKA
                     "
     gene            2003..2725
                     /locus_tag="PKW60_07100"
     CDS             2003..2725
                     /locus_tag="PKW60_07100"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HPJ99238.1"
                     /translation="MRYTGVDGCKTGWCVVTLPEIGPGAVRIEPDFRSVWLNHRGSST
                     ILVDMPIGLLEQREDGDPRRRCDALARQLLGKDATSRVFAVPCRQAIYAKDKAEAQVL
                     NQRHLARKISAQTIAIAPKIRQVDELLLSDPKARNKIFEVHPEILFYFLAGGNTLPRK
                     RSREGEKARIETLKRLEPRTDDILNQAAMLSKQYVAKDDVLDALACAVTALKAKGNFQ
                     TLPSDVPPDPKGLPMQMVYWMP"
     gene            complement(2815..3342)
                     /locus_tag="PKW60_07105"
     CDS             complement(2815..3342)
                     /locus_tag="PKW60_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99239.1"
                     /translation="MAKYRYRLDERHYLEAARKHNRRRFSWYFGPILVTLIALMFLVA
                     TLHIAVTEGECVPAVMTTVVILYILCVRRVAWWRWKNKLRKWILYKEESEITLTPGGV
                     HVITPKSDSTFDWQFWVKARRVRSGFLVYEEYDKLDSLVWLSDDSLVEGTVEDVDALF
                     RENIADYKPMRKGEQ"
     gene            complement(3387..4388)
                     /gene="hypE"
                     /locus_tag="PKW60_07110"
     CDS             complement(3387..4388)
                     /gene="hypE"
                     /locus_tag="PKW60_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HPJ99240.1"
                     /translation="MENSKRIVLAHGGGGALTRELIAEVLLTSLGRTADGLPDAAAVP
                     GADHLAFTTDSFVVKPLFFRGGDIGSLSVHGTCNDLAVSGAEPLALSLGLIIEEGFDI
                     ETLGRVVRSAASAAAASGVQIITGDTKVVARGEADQLFVNTAGVGIRRTHSDPKSLQP
                     GDCVLINGPIAEHGVAVMSEREGIAFGTQVRSDAGPVWPFVKALVDAGIEPRVMRDPT
                     RGGLAACCVELADDSGVTVELDEQAIPVRPETRAACDMLGLDPLTVANEGKIVVFVVE
                     SEAARALEVLRSTPGGEQATPIGRIVPRKRVSCVMRTRFGGERIVDMPYGEDLPRIC"
     gene            complement(4461..5513)
                     /gene="hypD"
                     /locus_tag="PKW60_07115"
     CDS             complement(4461..5513)
                     /gene="hypD"
                     /locus_tag="PKW60_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HPJ99241.1"
                     /translation="MILACPEPLLGRIRQAADALGRHVRIMEVCGTHTHAIGRGGLRS
                     LLPQTVELVSGPGCPVCVTSQRDIERLLLLARQPGVTLCSFGDMLRVPGLKTTLVEER
                     SRGAEVRVVYSPLDALELATREPERAVVFAAVGFETTAPGVASVVLQARERGLTNFTI
                     YASHKLIVPAMKAVLEGASRIEGFLTPGHVSVIIGPEAYEEVAREYKAPCVATGFEPS
                     DVLEGIAMVLECIAEKRAGSFVQYTRAGNARAWNLLLRVFDVADAEWRGLGVIPGSGL
                     VLNEAFAEFDAARRYALPDFPPVQLPRCRCGDVLKGVIHPPECPFFGNECTPRTPLGP
                     CMVSSEGSCAARYKYG"
     gene            complement(5494..5730)
                     /locus_tag="PKW60_07120"
     CDS             complement(5494..5730)
                     /locus_tag="PKW60_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HPJ99242.1"
                     /translation="MCLAIPAQITEIDGARGKVTLAGNVRDADFSLIDEPALGDWVLV
                     HAGFAIEKLEPEDARETLALFSELQEGMGDFGLP"
     gene            complement(5693..>6339)
                     /locus_tag="PKW60_07125"
     CDS             complement(5693..>6339)
                     /locus_tag="PKW60_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99243.1"
                     /translation="LHAAFGGEAVALCRAWFPEHAEQFPALAQMIARGVNCPQTTSMG
                     RLFDAAAALLGICQYNTFHAQAPMELEAQACLAPDEHKAYNAGIHCGEDGIFIIQSSD
                     IIRALVGDRRRRTSIPACAARFHNAIAHATLAVCERLRARSGLSTVALSGGVFANRFL
                     TERLVSLLESQGFTPLLNAAVPPGDGGISLGQAAAAAWSCIHHVFGHSSTDHRD"
BASE COUNT         1276 a         2000 c         1883 g         1180 t
ORIGIN      
        1 tatctgagca gcgaatcccg ctggtttgag gccaatctcc aggccgtcga gcgttttccc
       61 gacgccctct tccttcccgg tttctgggcg gaatacggca tgtgtaccga gccctcggca
      121 ttcggggcaa agtgcatttt tccagacaac gagttcccgt tcgctgagag ggcctgcgaa
      181 agcatcgaac aggccgccac gctccccaaa cccgatccca ggaccgacgg cctcgccccg
      241 ttcgtgctca aacggctgca gcatctcgaa ccacgcatcg tgaacgccgg ccacgccatc
      301 cgtttcgccg tcgcccgggg gccgctcaac attgcggggt ttctcctcgg caataccgaa
      361 ttattgctcg cgttgaagat ggatcccgat gcatctcaca gccttctgaa cacgttaacc
      421 tcctacctcg aagactggct caagcttcag gccgctgcct ttcccagtat cgacggcgtg
      481 ttgatgctcg acgacctggt gggattcctc ggaccggacg attttcagga attcgcccaa
      541 ccctatctca aacgcgcttt tgaggcgatt gacgcgcgcg tgcgtttctt tcataacgac
      601 gccgaagggc tgatctgtgc gccgtttcta gccggtaccg gcatcaacct cttcaatttc
      661 tcgcacaacc atccgctgca ggccatgcgc gaggctgcgg gcgaacaggt tgccctgctc
      721 ggcaacatcc cgccgaggga tgtgctggcc cagggcactc cggaccaagt gcggcagtca
      781 gtgcgcgaac tcctcgaatc cataaccgat acgcgcgggc tgatcctgtc ctgcggcggc
      841 ggcatgcctc ccggcgtgcc ggccgagaac atcgacgcct tcctggaagc cgcagcggac
      901 ggttgacggc cggcgcgtcc ggatcacccc cgtggctgcg ttaaagaagg gcagacacag
      961 gggtctgccc ctacgcgctg cgcagccaga tatgtatcag agcatgcccg gctcgtgttt
     1021 gcggtttgcg ccgggcgacg ggattcgcta tcctcagggg atgcacgaac tgtcatttgc
     1081 acaacagatt ttggaaacga ttcaacgcga aacggccggc tatgagggcg cacgcgtgac
     1141 ccgggtgtgc ctgcgcgccg acgaatatct cgcactcgaa cccgccagcc tccgattctg
     1201 tctggagagc attgccgtgg acaccatcat ggagggcgcg gtcatcgaga tcgaagaaga
     1261 accgctgccg ccgggcgctg agcggccgct gtttggcgct ggattggtta ttgaggagat
     1321 cgagctggat gagcaaaacg atcaaacttg agcaaaaagt gcaggccaaa ggccatgaaa
     1381 ttgccgcgga attgcgcgca atgttccgca aaaaaggcat attcgtcgtc aacctgatcg
     1441 gttcgcccgg cgcgggcaaa accagtttgc ttgaagccct ggcgccgcaa ctcaagggcc
     1501 gggcggccgt gatcgagggc gacctcgcca ccgacaacga caaacgccgc atcgaacagg
     1561 ctggcttgcg cgcctaccag atcaacaccg tgggcgcctg ccacctcgat gccgccatgg
     1621 tgcgcgacgc cctgaacgag ttctcgctcg atggaatcaa ccttttgttc atcgagaacg
     1681 tcggcaatct cgtgtgcccc gccagttttg aaatcggcga ggacatcaaa gtggccatcg
     1741 tgtcgactac cgagggcgaa gacaaacccg cgaaatatcc caaagccatg aaggtcgcct
     1801 cagccatggt catcaccaag gtggacctcc tgccgtatgt ctcctgcgac gtcgatcgca
     1861 tggagcgtga tgtgcgcgag ctggtgcccg atatgccggt tttccgcacc gccgctacca
     1921 ccggcgaagg caccggcgaa ttcctccaat ggattgaggc ccaggccgca gcgcgaaagg
     1981 cctagatcct gaaaggctta tcatgcgcta caccggtgtc gacggatgca aaaccgggtg
     2041 gtgcgtggtg actttgcccg aaatcggccc cggcgccgtg cgcatcgaac cggatttccg
     2101 ctccgtctgg ctgaaccacc gcggttcgtc cacgattttg gtcgacatgc ccatcggtct
     2161 tttggagcag cgcgaggatg gcgatccccg acgccgatgt gatgcgcttg cacgacaact
     2221 tcttggaaaa gacgcgacct cacgggtctt tgctgttccg tgcagacaag cgatttacgc
     2281 caaagataag gctgaagcgc aggttctgaa tcagcggcat ctagcgcgca agatttccgc
     2341 ccagacgatt gcaatcgccc ccaagatacg ccaggtagac gagctgctcc tcagcgatcc
     2401 gaaagcgcgg aacaagatat tcgaggtcca cccggaaatc ctcttctact tcctcgccgg
     2461 tggtaacacc ctgccgcgga agcggtcgcg cgagggcgaa aaggctagaa tagaaacctt
     2521 gaaacgcctg gagccacgca cagacgatat cctcaatcaa gcggcgatgc ttagtaagca
     2581 gtacgtcgca aaagacgatg tgctcgacgc cttggcgtgt gcggtaaccg cactaaaagc
     2641 gaaaggaaat tttcagactc ttccctccga tgtcccgccc gatccgaagg gactgccgat
     2701 gcagatggtc tactggatgc cgtgaagaag actgagccgc gggtcgatat cctgcgttca
     2761 gagtatcctc aggacgcaag ataactcagg gggagcttcc caagaggccc tctcctactg
     2821 ctcgcccttc cgcatgggct tgtaatcggc aatgttttct cgaaaaagcg catccacgtc
     2881 ttcaacggtc ccctcgacca gcgaatcgtc agagagccag acgagactat ctagtttatc
     2941 gtattcttcg tacaccagga aaccacttcg tacccgccgg gccttgaccc aaaattgcca
     3001 gtcgaaggtg gagtcgcttt tcggtgtaat gacatgaacc ccaccggggg tcagcgtgat
     3061 ttcgctctct tctttgtaca gaatccactt gcgaagcttg tttttccaac gccaccaggc
     3121 aacgcggcgt acgcacaata tgtacaggat aaccacagta gtcattactg ccgggacgca
     3181 ttcgccctca gtcacggcga tgtgaagcgt cgcgacgaga aacatcagcg caataagcgt
     3241 gacaaggatt gggccaaaat accaggagaa tcgccgtctg ttgtgtttcc gcgcggcctc
     3301 caggtagtgg cgttcatcta agcggtatcg gtacttcgcc atggagccag ccttattact
     3361 gcttcgtgat gtggctgttt actatactag caaatccgcg gcaaatcctc accatagggc
     3421 atgtcgacga tgcgttcccc gccgaagcgg gtgcgcataa cgcacgagac ccgtttgcgg
     3481 ggcacgatcc ggccaatcgg ggtggcctgt tcgccgccgg gtgtgctcct gaggacctcc
     3541 agcgcgcgcg cggcttccga ttcgaccaca aacacgacga tcttcccttc gttggcgacg
     3601 gtgagtggat ccagacccag catgtcgcac gcagcgcggg tttccgggcg caccgggatt
     3661 gcctgctcgt ccaactccac cgtcacgccc gaatcgtcgg ccagctccac acagcatgcg
     3721 gccaggccgc cccgggtggg gtcgcgcatg acgcggggtt caataccggc atcgacgagc
     3781 gctttgacga agggccagac cggccctgcg tcggaccgga cctgcgtgcc gaacgcgatc
     3841 ccttcgcgct cgctcatgac cgcgacgccg tgctccgcaa tggggccgtt gatgagcacg
     3901 caatcccccg gctggaggct tttagggtct gagtgggtgc ggcggatgcc cacccccgcc
     3961 gtattcacaa agagctggtc ggcttctccc cgcgcaacga ccttggtgtc gcccgtgatg
     4021 atctgcactc cggaggccgc tgccgcagat gctgctgagc gcacgacccg gcccagagtc
     4081 tcgatatcaa atccttcctc gatgatgagc cccagagaca aggccagtgg ttccgcgccc
     4141 gacacggcca gatcgttaca cgtgccgtga acactcaaac tgccgatgtc gccgccgcga
     4201 aaaaacagcg gtttcaccac aaacgagtcc gtggtgaacg cgagatgatc ggcgcccggt
     4261 accgcggccg cgtcaggcag gccatccgcc gtgcggccaa gcgatgtgag cagtacctcg
     4321 gcgatcagct cgcgtgtgag cgcgcccccg ccgccatgag ccaatacgat gcgcttcgag
     4381 ttttccatga tccgtacaac cgagtcctga atctgactac aaattcgctt ttctttggag
     4441 gaggaaaggc ttccccttct ttatccatac ttgtaccggg cggcgcacga accttccgag
     4501 gacaccatgc acggcccgag cggtgtgcgt ggagtgcatt cgtttccaaa gaaggggcat
     4561 tcgggcgggt ggataacgcc tttgaggacg tctccacaac ggcagcgggg cagttgcacc
     4621 ggcgggaaat ccgggagtgc atacctgcgc gctgcgtcga attccgcaaa cgcctcgttc
     4681 aagaccaggc cgctgccggg gatgacgccc agaccgcgcc attccgcgtc ggcaacgtca
     4741 aatacgcgca ggagcaggtt ccaggcgcgg gcattgccgg cccgcgtata ttgcacgaag
     4801 gagcccgcgc gcttttccgc gatgcattcc agcaccatgg caatgccttc aagaacgtcc
     4861 gacggttcga atcccgtagc cacgcatggc gccttatact cccgggcgac ttcttcgtag
     4921 gcttccggtc cgataatcac ggagacgtgg cccggcgtga ggaacccttc gatgcgcgac
     4981 gcgccttcga gcacggcctt catcgccggg acaatcagct tgtgtgaggc gtaaatggtg
     5041 aaattcgtca ggccgcgttc ccgggcctgc agaacgaccg atgcgacgcc cggcgccgtg
     5101 gtttcaaagc ctaccgccgc gaacaccacg gcgcgttccg gttcacgggt ggcaagctcg
     5161 agggcgtcca gcggcgaata gacgacacgc acctcggcgc cgcgggagcg ctcctcgacc
     5221 agcgtcgttt tgagccccgg cacgcggagc atgtcgccaa atgaacacag cgtaaccccc
     5281 ggctggcggg cgagcaggag aaggcgctcg atgtcgcgct gggaggtcac gcagaccggg
     5341 cacccggggc ccgataccaa ttcgacggtt tgcggcagca gcgaccgaag cccgcctctc
     5401 ccgatggcat gggtatgcgt gccgcagact tccatgatgc ggacgtgccg gcccagtgcg
     5461 tccgccgcct gcctgattcg gccgagtaat ggctcagggc aagccaaaat cacccatccc
     5521 ttcctgcaat tccgagaaaa gcgccagcgt ttcccgggca tcctcgggtt cgagtttctc
     5581 aatggcaaag ccggcgtgta cgagaaccca gtcgccgagg gccggctcgt cgatgaggct
     5641 gaaatcggcg tcgcgcacat tgcccgcgag cgtcactttg ccgcgggcgc cgtcaatctc
     5701 ggtgatctgt gctggaatgg ccaaacacat gatggatgca gctccatgca gcggcggccg
     5761 cctgccccag gctgattccg ccgtcaccgg gcgggaccgc cgcgttcagc aatggcgtga
     5821 atccctgact ttcgagcagg gaaacgagcc gttcggtcaa aaacctgttg gcgaagacgc
     5881 ctcccgacaa ggcgacggtc gacagccctg accgcgcccg gaggcgttcg cagaccgcca
     5941 gtgtagcatg cgcgatggcg ttatggaaac gtgcggcgca ggcggggatg gatgtgcgcc
     6001 ggcgccgatc ccccaccagt gcgcggatga tatcgctgct ctggatgatg aaaatcccat
     6061 cctcgccaca gtggatgccc gcgttatagg ccttatgctc gtcgggcgcc agacacgcct
     6121 gggcttcgag ttccataggc gcctgagcgt ggaatgtgtt gtactggcag atacccagca
     6181 acgccgcggc ggcatcgaaa agccggccca tgctcgtggt ttgagggcag ttgacgcctc
     6241 gcgcgatcat ttgagccagc gccgggaatt gttcggcatg ttcggggaac catgcgcggc
     6301 acaacgcgac ggcttcgcca ccaaacgcag cgtgaagat
//
LOCUS       DAOPBQ010000177         6266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10955_length_6266_cov_3.527451, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000177 DAOPBQ010000000
VERSION     DAOPBQ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10955_length_6266_cov_3.527451"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1476
                     /locus_tag="PKW60_07130"
     CDS             70..1476
                     /locus_tag="PKW60_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPJ99244.1"
                     /translation="MKKSLQCMPAKLAPAVRVMLVLTVMILCACRGGQYGGALIIETA
                     PEEGAVVVINGEPRGHTPLTIEGLPLGRVLIQVSKTGYRDAMTNAVVPERGREQRVHI
                     EMHPRTGWLTINSQPERARVVLSDGTELGTTPILRRSVPAGQISYTLELEDYEPFSGT
                     ITVHEDLLASTSHKLEPKPAQLEVTSVPERAQIIVNKKLQDVVTPATLSLRPGTYVVG
                     LKVKGFVEAERSIELGPNEQETMHFELNVGEAPPGMVLVAGGEFICGVNNAAPDERPQ
                     RKVHLEAFFIDKYEVTNEQYQAVVPAHTFPQGREAYPVTGITFQLATAYASAIGKRLP
                     TELEWEKAARGTDGRTYPWGNDFDRKNCNSLSSAVLELREVGQYPAGNSPYGCADMAG
                     NVLEWTSSWYEAYPGNPDVTKDYGQIYKVLRGGSFTSEPFDVRCARRHFDRMDVARKD
                     YGFRCVMDIEAWDARGRR"
     gene            1700..3970
                     /gene="pilM"
                     /locus_tag="PKW60_07135"
     CDS             1700..3970
                     /gene="pilM"
                     /locus_tag="PKW60_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPJ99245.1"
                     /translation="MASLKSPRLQRRLVLDIGSSALRVCELTKTKTGFQLTKYLQREL
                     NLPPSTPEPERAARVSEVLNDMLKEAKIRARKTVFGVPGQSVFTRTRALPPVPEHKVP
                     QIVRYEIQQQIPFSLDQIALGYQVLARTEAGGYDVLMAAIKVDVVDKRLDAIKSAKRA
                     VGIVDVCPVAAYNWLKTAGEFGAHGECVALIDLGASTTDIVIEREGQFRFTRSLNLGG
                     NDITNAIGNTFNMDFEEAERLKRQRGFAPTGDAQRDGKGGEVIGQLLNRLVTEINRSF
                     AYFRSQPGGGPVSRVVITGGGACLRNIVPYFQRQLNMEVRIAQPLAGLAIAPAAQAVN
                     EHPEQAAVVLGLALRNCGEVPIGINLIPPRIIEGARRKQQYVYWAASLVTVLAIFATI
                     IPDMQNKIVEVERHTNMARDVIGKYDPRLVDNPTSACQYDGDLNIAKNYIKGIQDSIA
                     SIDSFYLRRRPWLEYLNALNDARQRDPVWFTQVFSTTLSVYDPHRTSRAGALYDDDDD
                     DDYDRPQRPPAASAIQRRPVGGPPVPDETELVRVLDSSGFPGITPDQAGSRFRSGGRL
                     GGGGRYDDDDEDGRGGAFSQPVSSMGAYAQPGRPGAMGPGGTGAAERLDPMPLPNGLE
                     IHGYAADEGVVLNYVERLKARGEFVEVQLDGRSVNPVPNTDLGQSFTVGLPAAVAGSP
                     TVVTFVILVQVAGEPLKATEVGNTGIMSGSGPAGGAGGRGIGLRGGAGTDTQLRRSTP
                     GTRTRGGGQGARRRDN"
     gene            3970..4914
                     /locus_tag="PKW60_07140"
     CDS             3970..4914
                     /locus_tag="PKW60_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99246.1"
                     /translation="MKKEYVIFIGAAIVMLLVIGGGYQFYLKPTAERLDQKKQEYRSY
                     DTKLMDLEERYGGYVPEDVVSAWMLQLTPWYNTIDSRGDLFGLGRVEFEEVPTNTLPK
                     FHYAGQYEAMMEALRVEALEKGLYLPMLDMDVPSTQIENMTMTKDEVEEYLRQIAIGC
                     NLVRLLLAHDPLEIQAVYLWPDRVPAPRIKAVTVGYAFTARMSSVAKLLDDICSQQRY
                     ASVDSFTIVNENLLMGADPPLMVQLVLTQATYTPVEQLSPEAILAQAKDPTSEQSSLL
                     KMLQGQAGSFGDEDDDLEYARRKPLTKWQVFRKMYLPF"
     gene            4974..5711
                     /locus_tag="PKW60_07145"
     CDS             4974..5711
                     /locus_tag="PKW60_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99247.1"
                     /translation="MDILRKLGDWGWMNKERLVLAILVIFLIYRIYVVMNPTTIEEQT
                     AQAAAAQRSTAAQAAMPGPPAIPGMPSPSAWDDDDEDEDEDYPGGRPGVVQSSQRPQA
                     QRAASGDQPLVFTQHMPPKRIPDPTNLPAEWTEAQGKPPVPPANPLPPSPVAYNALVK
                     ENPFTAATVERSTPDGPGGRPNLRLLAIREWGDTYRAQIQTRTRKWYAAGESFETYQL
                     VSIDPDAGTVVIFSEQDGQNYTFALNR"
     gene            5769..>6266
                     /locus_tag="PKW60_07150"
     CDS             5769..>6266
                     /locus_tag="PKW60_07150"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ99248.1"
                     /translation="MDTQKQRRKAVRMSAATVLCVCLTAVWWCPGAFAQAGGGTKADG
                     DGVTAEVQAGQVEVLEAPSAALGELASAEAETSVPPEQLHYRQGVSLYNQGLYREALE
                     SFERALAMSGDYTLARQMAEKCEAKLNLEAAGADPNAVPKFETLVPAELETVSAGGGE
                     MLLSVE"
BASE COUNT         1305 a         1739 c         2067 g         1155 t
ORIGIN      
        1 gggcggctct cccggggggt gttggatgcg tgagccgcgg agcgtatggg tgggcttaag
       61 gacacgcgga tgaagaagtc gctgcagtgc atgccggcaa agctggcgcc agcggtgcga
      121 gtaatgctcg tgctgacggt gatgattctg tgcgcttgcc ggggagggca gtacggcggt
      181 gcgctgatca ttgaaaccgc tccggaagag ggtgcggttg tcgtcatcaa cggcgagccg
      241 cgcggccata cgcccctgac catcgagggc ctgcccctgg gccgcgtcct tattcaggtc
      301 tcaaagacgg gctaccggga tgccatgacc aatgccgtcg tgccggaacg gggccgcgaa
      361 cagcgcgtgc acattgaaat gcacccgcgc accggatggc tcaccatcaa ttcgcaaccc
      421 gagcgtgccc gggtcgttct cagcgacggc accgagctgg gcaccacacc gatcctccgg
      481 cgcagtgttc ccgcggggca gatcagctat acgctggaac tggaggacta tgagccgttt
      541 tcgggaacga tcacggtgca tgaggacctg ctcgcctcaa ccagccacaa gctggagcca
      601 aaacccgccc agctggaggt gacctcggtg cccgagcgtg cgcaaatcat tgtaaataag
      661 aaacttcagg atgtggtgac ccccgcaact ctgtctctgc gcccgggtac ctatgtggtg
      721 gggctgaagg tcaagggctt tgtggaggct gaacgctcca ttgaattggg ccccaacgag
      781 caggagacga tgcatttcga gctgaatgtg ggggaggcgc ctccgggcat ggtgctggtg
      841 gccggcggag agtttatctg cggggtgaac aatgcggcac cggacgaacg cccccagcga
      901 aaggtccatc tggaggcctt ctttattgat aagtatgagg taaccaacga gcagtatcag
      961 gccgtcgtgc ctgcccatac cttcccgcaa ggccgcgaag cctatcccgt tacaggcatt
     1021 accttccaac tggcaacggc ttatgcatcg gccatcggca aacggctccc caccgagctc
     1081 gaatgggaaa aggctgcccg gggcacggat ggacgcacct acccgtgggg aaacgatttt
     1141 gaccggaaaa actgcaactc cctctcctcg gccgtccttg aattgcgtga agtggggcag
     1201 tatcctgccg gaaactcccc gtacggctgc gcggatatgg cgggaaatgt cctggagtgg
     1261 acgtcgagct ggtatgaagc ctatcccggt aatccggacg tgaccaagga ttatggccag
     1321 atttataagg ttctccgggg cggctcgttt accagcgaac catttgatgt gcgctgcgcc
     1381 cgccgccatt ttgaccgcat ggatgtagcg cgtaaggatt acggattccg atgtgtgatg
     1441 gatattgagg catgggatgc gagaggccgg cgctaagcaa aacagggcag agggatcaat
     1501 tgtctggtgc gatttaactc ttgacacaca tggctttctt tattacaatc atttgtaagg
     1561 gtttatgact tggggccgat catgaggggg tgcagatttc gcgccgcctc gcacggcaaa
     1621 cgaggttggc tttgtgctgg aatcgggttt gcggctggtt acccggcact gctgccaaaa
     1681 gtacggcgga aagtaacgaa tggcgtcttt aaaaagtcct cggctccaac ggcggctcgt
     1741 actggacatt ggttccagtg cgctgcgggt gtgtgagctc actaagacaa agacgggctt
     1801 ccagctgacc aagtacctgc agcgggagct taatttgccg ccttccacgc ctgaacccga
     1861 gcgggcggcc cgtgtctcgg aagtgctcaa cgacatgctc aaagaggcga aaatccgagc
     1921 acgcaaaacc gtgttcggtg tgccggggca gtcggtgttt actcgcacgc gcgcactgcc
     1981 tcccgtgcca gagcacaagg ttccccagat cgtccgctac gaaattcaac agcagattcc
     2041 attctctctc gatcagatcg cgctgggcta tcaggtgctt gcccgcacgg aagcgggcgg
     2101 ttatgacgta ttgatggccg cgatcaaggt ggatgtcgta gacaaacgcc tggatgcgat
     2161 caagtccgcg aagcgcgcgg tcggtattgt ggatgtctgc cccgttgccg catataactg
     2221 gctgaaaacg gcgggagaat ttggcgccca cggcgagtgc gtagcgctca tcgatctggg
     2281 ggcgagcacg acggacatcg tcatcgagcg cgaggggcaa ttccggttca cgagaagcct
     2341 gaacctcggc ggcaatgata ttaccaacgc gatcggcaat acgttcaaca tggacttcga
     2401 agaagccgag cgcctgaaac ggcagcgcgg ctttgcgccg acgggcgacg cgcagcgtga
     2461 cggcaagggc ggcgaggtca ttggacaact gctgaaccgg ctggttaccg agatcaaccg
     2521 gtcgttcgcg tatttccgct cacagccggg cggcggaccg gtgtcccggg tggtcattac
     2581 gggtggcggc gcctgcctgc gcaacatcgt gccgtacttc cagcgccagc tgaacatgga
     2641 ggtccggatc gcacagccgc tggccggact ggcgatcgcg ccggcggcgc aggcggtgaa
     2701 tgaacacccc gaacaggcgg ctgtggtgct ggggctggcc ttgcgcaatt gcggtgaagt
     2761 gccgatcggc atcaatttga tcccgccgcg gattatcgaa ggggcgcgcc gtaagcaaca
     2821 gtatgtctac tgggctgctt cgctggtgac ggtcttggcc atctttgcca cgatcatccc
     2881 ggacatgcag aacaagatcg tcgaagtcga acggcatacc aacatggccc gcgatgtcat
     2941 cggaaaatac gacccgaggc tcgtcgacaa cccgacctcg gcctgccagt atgacggcga
     3001 tctcaacatc gccaaaaact acatcaaagg catccaggac agtatcgcca gcattgactc
     3061 gttttacttg cgccggcggc cctggctgga atatctcaac gcattgaatg acgcacgcca
     3121 gcgggatccg gtgtggttta cccaggtatt cagcacgacg ctcagtgtgt acgatcccca
     3181 ccggacgagc cgggcgggcg ccctgtatga tgatgatgac gatgacgact atgacaggcc
     3241 gcagcggccc ccggcagcca gcgcgattca gcgccggccg gtgggcgggc ctcccgtacc
     3301 cgacgagacg gaattggtgc gggttctcga tagttcggga ttccccggca tcaccccgga
     3361 ccaggctggc agcaggttcc ggtcgggcgg ccgcctgggg ggcggcggaa gatatgacga
     3421 cgacgatgag gacggcaggg ggggcgcatt cagccagcca gtttcttcaa tgggtgcgta
     3481 tgcccagccg ggccgcccag gcgccatggg tcctggaggt acaggtgctg cggaacggct
     3541 cgaccccatg cccctgccca atggcctgga aatccacggg tacgcggccg atgaaggcgt
     3601 ggtgctgaac tatgtcgagc gcttgaaggc tcgaggcgag tttgtcgaag tgcagcttga
     3661 cggccgcagc gtgaatccgg tgccgaacac agatttgggc caatcgttca cggtggggct
     3721 gccggccgct gtcgcgggca gcccgacggt ggtcactttt gtgattctgg tgcaagttgc
     3781 gggcgagccc ttgaaggcta cggaagtcgg caataccggg atcatgagcg gcagcgggcc
     3841 ggcgggtggc gccggcggac gggggatcgg tttgcggggc ggcgctggca cagataccca
     3901 gcttcgtcgc agcactccgg gcacgcggac cagaggcggc ggccagggtg ccaggagaag
     3961 ggacaactga tgaagaaaga atacgtcata ttcatcggtg ccgcgatcgt gatgctgctt
     4021 gtgatcggcg gaggctatca attttatctg aagccgacgg ccgagagact ggatcagaaa
     4081 aagcaggaat accgcagcta cgatacgaag ctgatggatt tggaggaacg gtacgggggc
     4141 tatgttccag aagatgtcgt atctgcctgg atgctgcaac tgaccccctg gtataacacc
     4201 atcgattcgc gaggcgatct tttcggcctc gggcgggtgg agtttgagga agtgcctacc
     4261 aatacgctgc cgaaatttca ttatgccggg cagtatgagg ccatgatgga ggcattgcgc
     4321 gtcgaagcgc ttgaaaaagg gctgtacctg cccatgctgg atatggacgt gccctcaaca
     4381 cagatcgaaa acatgacgat gacgaaggat gaggtggaag agtatctgcg ccaaatcgcc
     4441 attgggtgca atctggtgcg gttgctgctt gcccatgatc cgctggagat tcaggccgtc
     4501 tacctctggc cggatcgggt gccggcgcca cggatcaagg ccgttaccgt gggctacgcc
     4561 ttcacggctc gcatgagctc agtggcgaaa ctgctggatg atatctgcag ccagcagcgc
     4621 tatgccagtg tggattcttt caccatcgta aatgagaatc tcctgatggg ggcggatccg
     4681 ccgttaatgg tgcaactggt gttgactcag gccacctata ccccggtgga gcaattgagc
     4741 ccggaagcca ttctggcgca ggccaaagat ccgacgagtg aacaaagcag cctgctcaaa
     4801 atgctgcagg gccaggcggg cagttttggc gatgaggacg acgaccttga gtatgcgcgg
     4861 cggaaacctc tgacaaaatg gcaggtgttc cggaaaatgt acttgccctt ctagacgttg
     4921 caggcgtcag gggggtgcgt gcaaggatga cggacagacg gatagggtga agcgtggaca
     4981 tacttcgaaa actcggtgac tgggggtgga tgaacaagga acggctggtg ctggccatcc
     5041 ttgtgatatt cctcatctac cggatctacg ttgtcatgaa cccgaccacc attgaggagc
     5101 agaccgccca ggcggcggcc gcgcaacgca gcaccgccgc gcaggcagcc atgccgggcc
     5161 cgcctgccat ccccggcatg ccgagcccca gcgcgtggga cgacgacgat gaggatgagg
     5221 acgaagacta tccgggaggg cgcccgggag ttgtccaaag cagtcagcgt ccgcaggcac
     5281 aacgtgcggc ctcgggggat cagcccctgg tgttcacgca acacatgccg cccaagcgga
     5341 tcccggatcc gaccaacttg cctgcagagt ggaccgaggc gcagggaaaa ccgccggtac
     5401 cgccggcaaa ccccctgcca ccgtcgccgg tggcgtataa tgcgcttgtg aaagagaacc
     5461 cgtttacggc agcaaccgtt gaaaggagca caccggacgg gccgggtggc cggccgaacc
     5521 tgcgtcttct tgccatcaga gaatgggggg acacgtaccg cgcccagatc cagacacgga
     5581 cacggaagtg gtatgcagct ggggaatctt ttgagacgta tcaactggtc tcgatcgatc
     5641 cggatgccgg aacggtggtg atattttcag agcaggatgg ccaaaactat accttcgctc
     5701 tgaatcgttg agactccgac agtgttgttc tataatctag cgaactctag cgggaggaag
     5761 cgagcgcaat ggatacgcaa aagcagcggc gtaaagccgt gcgaatgagt gccgccacgg
     5821 tattgtgtgt gtgtttgacc gcggtgtggt ggtgcccagg agcattcgca caggccggtg
     5881 gtggtaccaa ggctgacggc gatggggtaa cagccgaggt gcaggccggg caggtcgagg
     5941 tgctcgaagc gccatcggca gctttgggcg agctggccag tgcggaggcc gaaaccagcg
     6001 ttcctccgga gcagctccat taccgccagg gggtatccct gtacaatcag gggctttacc
     6061 gggaggcgct ggaaagcttc gagcgcgccc tggcgatgag tggggattat acgctcgcgc
     6121 gccagatggc cgaaaagtgc gaagcgaagc tcaacctgga ggccgcgggc gccgacccca
     6181 atgcggtccc caagttcgag accctcgtgc cggccgagct cgagactgtc tcggcggggg
     6241 gcggcgaaat gctgctcagc gtggag
//
LOCUS       DAOPBQ010000178         6264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10963_length_6264_cov_3.956193, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000178 DAOPBQ010000000
VERSION     DAOPBQ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10963_length_6264_cov_3.956193"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PKW60_07155"
     CDS             <1..728
                     /locus_tag="PKW60_07155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPJ99249.1"
                     /translation="SGFLYAAAGGVAAAILYARLKVPGVHYRRSFFVIRRKFRLYYVL
                     EFLFGARKQIFITFGPWVLIHVYGLSPGNIAFLLMLASIIGLVFKPLAGWVMDAWGER
                     LVMVGDGLVLTLVCLGYGFAGSFPGGPKVALCIAGSCFILDNLLFALSTGRTVYVSRL
                     SSSPDELTGTLSLGVTMNHVASMVIPFWAGLMWKELGFQVVFASAGVLALLLACFSWF
                     VPGKRARAPIMGGDLYTTEGQAP"
     gene            845..1522
                     /locus_tag="PKW60_07160"
     CDS             845..1522
                     /locus_tag="PKW60_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPJ99250.1"
                     /translation="MEKGQANRATDPANGAEARLLEGALSIFSKKGYEGASVREIIEE
                     AGVTRPVLYYYFKNKEDLFRRLVRNCLAEMIADADAALDQGETCQSCLEMLICKAFER
                     AERNLDEVRLLVHVIFTPPGQAPGILDEPLLDEWFTRLVKVMDDGLLRGELSGGEPEA
                     LALAFNALMDFHMMVKSRYPSVTLTPDLGKGLVALFLRGAGPGGVERLPLLNTFQVQP
                     REGGAWS"
     gene            1532..2626
                     /locus_tag="PKW60_07165"
     CDS             1532..2626
                     /locus_tag="PKW60_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPJ99251.1"
                     /translation="MKQDKSSMGRTLWSILICLGITAMVTAVVAVTAHVAPSEAKAGH
                     AGAAERLPQVSVRVLKSQTVEDQLVLTGSVQPWEEVILSSEVRGKIEKKGIELGDTVT
                     SGQELYRVDTESIRARLGQAQAQHKLALQELERIERLARSGAGTAQSLDSAVANRDVM
                     DSSLRMLQIELDKSVVRASFQGVVDAVLVEQDEFVDVGTPLVRLVQVHKVKMMFGIPE
                     RDIACFKMGDPVRVRMDAIPDRTFNGSIYRIATSADRSTHTFKAEVEVDNADGLLKPG
                     MIARGLFVRKTYPDSLMIPLFTAILLDDQRVAFVEKDGIAELRPIQVGLVQGSSVQVT
                     EGLAPGERLIVKGQYEARPGEKVDVQEVLE"
     gene            2623..5805
                     /locus_tag="PKW60_07170"
     CDS             2623..5805
                     /locus_tag="PKW60_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPJ99252.1"
                     /translation="MIISNVAINRSTTVFVLLVLILVAGVYSYLSLPRESNPEVVIPY
                     ITVSVPYEGVAPEDIESLITIPIERRLAGLSGVKKMRSTSVEGLSYIFLEFEPDVDID
                     DALQKVRDKVDLAEPDIPKEADDAVISEINTAEFPIMIMSLSGKLPLSMLDQIAGDLE
                     DQIETIEGVLDVEVIGGVEREIQIEVDPERVAQYGISFGDLISVVAQENVNTPGGTMD
                     LGEAKFVMRTPGEFKNPDDINDLVVKQSPEGTVFLRDVAKVFDGFKDIETISRLNGEP
                     SVTLTVIKRSGANIIEIADQVQKEIDRMLAVLPKGLSVTVTWNEANYIRDIVSELENG
                     ILSGMILVLLVIFVFLGFSNAVFVALAIPVSMLITFVVLQMSGVTLNMVVLFSLILAL
                     GMLVDNGIVVVENIYRHLSMGMSRVEAAKAGTAEVAWPITTSTLTTVAAFVPFFFWPG
                     IFGEFMYYVPYTVIVALLASLFMGLVVNPALAAVFMRAHDGKTMRRITGAGFVMRLYA
                     ATLRTALRWRAVTVLSAVALLIMITTLFFSTAETIFLPVTEPPQAYIDIECAEGTNLA
                     TSDQYARQVEELIAPYRPYVDSVVTSVGSLGVTPQAGHSTGSSKATHISRISLDFPKL
                     DDCTKLPSEVLRELRARLTGLSGADLRIDQMTMGPPTGPPINVEISGDNFETLARLAQ
                     EVRQEIKDVPGLVDLRDDYNKGKPEVQVVVDRQEAWRMGLSTQLIGLTVQAAINGRKA
                     ADFREGDEEYDVTVRFPKAFREDLGNINTMNLIAIDGRRVPFSSVAHIEQGAGLGSIK
                     RIDRKRTVTVSADVHPDFNQQRLLQAVQAKLEDFPLPPDYSFAYTGENEDMEETQFFL
                     FKAFVVAIFLIALVLVTEFNSLVQPFIILTSVVLSLAGVFLGLYIFNMPFGILMSGVG
                     CVSLAGVVVNNAIVLIDYTNQLRARGLELNEAIVTAGVTRFRPVMLTAVTTVLGLVPM
                     AIGVSFDFRAMRLIVGGEMSQWWGGMAVAVIFGLTFATMMTLIVVPTLYSAMAGLSET
                     IGTVKSRPQTSQGQFPEPDAAKEKLAEEQA"
     gene            5807..>6264
                     /locus_tag="PKW60_07175"
     CDS             5807..>6264
                     /locus_tag="PKW60_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99253.1"
                     /translation="MPRYTGLALGAILLLVGAAFFLSIHSGETGSPQFFGEMWLPLDA
                     ASRLVLRPVSPGTENPEPRPLDIVPLAGQEVMTLFPEAPPRLTNLQQAYETAQLPFSV
                     TLKAVEFETGQVIPAHLKLSDSDGERMLPFQSGESVDLSGQTFVMREIRP"
BASE COUNT         1227 a         1656 c         1995 g         1386 t
ORIGIN      
        1 tgtccggatt cctgtacgct gctgccgggg gcgtcgcagc ggccatcctg tacgctcgat
       61 taaaagtccc gggagtgcat taccggcgtt cttttttcgt gattcggcgg aagttccggc
      121 tctattacgt gctcgaattt ttgtttgggg cacgaaaaca gatcttcatc acgtttggcc
      181 cctgggtgtt gattcacgtc tatgggcttt ccccggggaa cattgcgttc ctcctgatgc
      241 tggcctccat catcggcctc gttttcaagc cgcttgccgg ttgggtgatg gacgcctggg
      301 gagaacgcct cgtgatggtg ggtgatggcc tcgtgctgac gctggtctgc ctgggctacg
      361 ggttcgcggg cagttttccc ggcggcccga aggtggccct gtgtattgcc ggttcgtgct
      421 tcattctcga taacctcctg ttcgcgttga gcacgggacg cacggtgtat gtctcccgtt
      481 tgtcatccag cccggatgag ctgaccggca ccctgtcgct gggcgttaca atgaaccatg
      541 tggcctccat ggtgattccg ttctgggcgg gcctgatgtg gaaggagctg gggtttcagg
      601 tcgtcttcgc ttctgcgggc gtactggcgc ttctgctcgc gtgtttctcg tggttcgtgc
      661 ccggaaaacg ggcccgggcg ccgatcatgg gcggtgatct gtacaccacc gaaggccagg
      721 cgccgtgagc ggcattgcgc cggcagaaaa gtgccgggat ttccaagaga caacctgctg
      781 gactttcgtt cgtttgggtt gtataatccc ttcggttacc taacgaacgg taggtcagat
      841 gcagatggaa aaagggcagg ccaatcgcgc gactgatccg gcaaacggag cggaggctcg
      901 tttgctcgaa ggcgccctaa gtatattttc taaaaaaggt tacgagggcg cgagcgtgcg
      961 ggagattatt gaggaggcgg gagtcacccg gcctgttctt tattactatt tcaagaataa
     1021 ggaagattta ttccggcggc tggtgcgaaa ttgccttgcg gagatgatcg cagacgcgga
     1081 tgccgctctc gaccagggcg agacgtgcca atcttgcctt gaaatgctga tctgcaaggc
     1141 gttcgagcgg gcggaacgca acctcgacga agttcgcctt ctggtgcatg taattttcac
     1201 tccgccggga caagcgccgg gcatcttgga cgaacccctg cttgatgagt ggtttacccg
     1261 gctcgtgaag gttatggatg atggtctgct gcggggcgag ttgtctgggg gcgagcccga
     1321 agctctggcg ctcgcattta atgcgttgat ggatttccat atgatggtga aatcacgcta
     1381 tccatccgtc acactgacgc ccgatttggg aaaaggcctg gtagcccttt ttttgcgtgg
     1441 agcaggccct ggaggagtgg aacggcttcc gcttctgaac accttccagg ttcaaccccg
     1501 cgagggcggt gcgtggagct gaggacgatt tatgaaacag gacaagagca gtatggggcg
     1561 gacgctgtgg agcattctta tttgcctggg aatcacggcc atggtaacgg ctgtggtcgc
     1621 cgtcacggcg catgtggcgc cttccgaagc gaaggcgggg catgcgggcg cggccgagcg
     1681 cctgccgcag gtcagtgtgc gcgtcctgaa aagccagacg gttgaggacc agttggtgct
     1741 gacgggcagc gtgcaaccct gggaagaagt gatcctcagc tcggaagtgc gcgggaagat
     1801 cgaaaagaag ggcattgaac tgggcgacac ggtgacgagt ggacaggagc tgtaccgggt
     1861 ggatacggag tccattcgcg cccgcctggg gcaggcacag gcgcaacaca aactggcgtt
     1921 gcaggaactc gaacggattg agcggcttgc ccggagcggc gccggaacgg cccaaagcct
     1981 cgacagcgcg gtagcgaatc gcgacgtgat ggattcgtcg ctgcgcatgc tgcagatcga
     2041 actggataaa agcgtcgtca gagcctcgtt ccagggcgtg gtggatgccg tgctggtgga
     2101 acaggacgaa ttcgtcgacg tcggcacgcc gctggtgcgg ctggttcagg tgcacaaggt
     2161 caaaatgatg ttcggcattc cagagcgcga tatcgcctgc ttcaagatgg gcgacccggt
     2221 acgggtgcgc atggatgcca tacccgaccg cacgttcaac ggaagcatct accggattgc
     2281 gacgagcgct gacaggagca cacacacatt caaggcggag gtggaggtgg ataacgccga
     2341 cggcctcttg aagcccggca tgatcgcccg cggcctgttt gtacggaaaa cgtatccgga
     2401 ttccctgatg atcccgctgt ttacggcgat actgcttgac gatcagcgcg tggccttcgt
     2461 ggaaaaagac ggcatagccg aattgcggcc cattcaggtg gggctggttc agggcagctc
     2521 cgtgcaggtc acggaaggcc tggcgccggg ggaacggctg attgtgaagg ggcagtacga
     2581 agcgcggccc ggggaaaagg tggacgttca ggaagtcctt gaatgatcat cagcaatgtt
     2641 gccataaacc gcagcacgac ggttttcgtc ttgctggtcc tgattctggt cgcgggcgtc
     2701 tacagttacc tgagcctgcc ccgtgaatcc aatcctgaag ttgttatccc ctacatcacc
     2761 gtgagcgtgc cgtacgaggg agtcgcaccc gaggatatcg agtcgctcat tacgatcccc
     2821 atcgagcggc gcctggccgg cctgtccggc gtcaaaaaga tgagatccac gagcgtggag
     2881 ggcctgtcct acatctttct cgaatttgag ccggacgtcg atatcgacga cgcactccag
     2941 aaagtccgcg ataaggtcga cctggcggag ccggacattc cgaaagaggc ggatgacgcc
     3001 gtgatcagtg agatcaacac ggctgaattt ccgatcatga ttatgtcgct gtcgggaaag
     3061 cttccacttt ccatgctgga ccagattgcg ggagatctgg aagatcagat tgagacgatc
     3121 gagggcgttc ttgatgtgga agtcatcggg ggcgtcgaac gggaaatcca gatcgaggtg
     3181 gatccggagc gcgtcgccca gtatgggatc tcgttcgggg atttgatcag cgtggtggcc
     3241 caggagaacg ttaatacgcc cgggggcacg atggatcttg gggaagcgaa gtttgtgatg
     3301 cgcacgccgg gtgaattcaa aaacccggac gacatcaatg acctcgtggt gaagcaaagc
     3361 cccgaaggca cggtattcct gcgggacgtg gcgaaagtct tcgacggatt caaagatatc
     3421 gagacgattt cgcgtctgaa cggcgagccg tcggtgacct tgacggtcat caagcgttca
     3481 ggtgcgaaca ttattgaaat cgccgatcaa gtgcagaaag agattgaccg gatgctggcc
     3541 gtgctcccca aagggctgag cgtcacggtg acgtggaacg aggcaaacta catccgagac
     3601 atcgtatcgg agcttgagaa cggcatcctg agcggcatga ttctggtgct gctggtcatt
     3661 tttgtgtttt tgggtttctc caatgcggtt ttcgtggcgc ttgccattcc cgtatcgatg
     3721 cttatcacat ttgtcgtcct tcagatgagc ggcgtcacgc tcaacatggt ggtgctgttc
     3781 agtctgattc ttgcgctggg catgctggtc gacaacggga tcgtggtggt ggagaacatc
     3841 taccgccatc tgtcgatggg gatgagcaga gtcgaggccg ccaaggcggg caccgccgag
     3901 gtggcctggc ccatcaccac gtcaaccctg actaccgtgg ccgcgtttgt gccgtttttc
     3961 ttctggccgg gtattttcgg cgaattcatg tattacgtcc cgtatacggt tatcgtagcg
     4021 ctgctggcct cgttgttcat ggggctggtc gtcaatccgg ccctcgccgc cgtgttcatg
     4081 agggcgcacg acgggaaaac gatgcgccgc atcaccggcg ctggattcgt gatgcggttg
     4141 tatgccgcca ccctgcgcac ggcgttacgc tggcgtgcag tgactgtgct ctcggctgta
     4201 gctctgctga taatgatcac cactttgttc ttctcgacgg ccgagacgat ttttctgccg
     4261 gtaacggagc cgccgcaggc gtacattgac attgagtgcg cggaaggcac aaatctcgct
     4321 acgtcagacc aatatgcgcg gcaggtcgaa gagctgattg ctccgtatag gccttacgtg
     4381 gacagtgtgg tcacaagcgt gggttcgctg ggcgtcacac cgcaggccgg ccattccacg
     4441 ggaagttcaa aggccaccca catcagccgg atctcgctgg atttccccaa gctcgatgac
     4501 tgcaccaagc ttccgtcaga agtccttcgc gaacttcgag cccggttaac gggattgagc
     4561 ggcgcggatc tccgaattga ccagatgacg atgggaccgc cgaccggccc gcccatcaac
     4621 gtggaaatca gcggagacaa tttcgagacg ctggcgcgtc tggcgcaaga ggtccggcag
     4681 gaaatcaagg atgtgccggg gctggtcgac ctgcgcgacg attacaataa gggaaagccg
     4741 gaggttcagg tggttgtaga ccgccaggaa gcatggcgca tggggctcag cacgcagctc
     4801 atcgggttga ccgtgcaggc cgcaatcaac ggccggaaag cggccgattt ccgcgaaggc
     4861 gacgaagaat atgatgttac ggtgcggttt cccaaggcat ttcgtgaaga tctgggaaac
     4921 atcaatacca tgaatctcat cgcgatcgac gggcggcgag tgccgttttc ctctgtggcg
     4981 catatcgagc agggggcagg cctgggcagc atcaagcgga ttgaccgcaa acggacggtc
     5041 acggtgtccg cggatgtgca tccggatttc aaccagcagc gtttactgca ggcggtccag
     5101 gccaagctgg aagactttcc gttgccgccc gattactcgt tcgcgtatac gggcgaaaac
     5161 gaagacatgg aagagacgca gtttttcctg ttcaaggctt ttgtggtggc gatttttctt
     5221 attgcattgg tgctggttac agaattcaat tctctggtgc aaccctttat tattctgacc
     5281 tccgtggtat tgtctctggc gggcgtattt ctggggctgt atatttttaa catgcccttt
     5341 ggcattctga tgtccggggt aggttgcgtg agcctcgcgg gggtggtggt caataacgcg
     5401 atcgttttaa ttgattacac aaatcagctg cgggcccgcg gcctggagct gaatgaggcg
     5461 atcgtgaccg ctggtgtgac acggttccgc ccggtgatgc tgaccgcggt aaccacggtg
     5521 cttggcctgg tgccgatggc catcggagtc agctttgatt tccgcgcgat gcgcctgatt
     5581 gtcggcggcg agatgagcca atggtggggc ggcatggccg tagcagttat tttcggcctg
     5641 actttcgcca ccatgatgac gctcattgtg gtgcccacgc tgtacagtgc catggccgga
     5701 ttgtccgaaa ccataggcac ggtcaaatcg cggccccaaa cctctcaggg gcaattcccc
     5761 gagccggacg ccgccaaaga gaaattggcg gaagagcagg cttgatatgc cccgctacac
     5821 agggttagcg ctcggcgcca tacttcttct cgtgggcgcg gcgtttttcc tctccatcca
     5881 ctcaggcgag accggctcac cgcagttttt tggtgagatg tggttgccat tggatgctgc
     5941 atcgcgactt gttctgcggc cagtctctcc gggaaccgaa aacccggagc cgcgcccgct
     6001 cgatattgtg ccgctggccg ggcaggaggt gatgacgctc tttccggagg cgcctccacg
     6061 ccttacgaat ctccagcagg cctatgaaac ggcgcaattg cctttttccg tgaccttgaa
     6121 agcggtcgaa tttgaaacag gccaggttat tccggcccac ctgaagcttt ctgatagtga
     6181 tggcgaacgc atgctcccgt tccaatcggg tgaatccgtg gacctgtccg gacagacgtt
     6241 cgtcatgcgg gaaatccgcc cctg
//
LOCUS       DAOPBQ010000179         6261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_10970_length_6261_cov_4.101354, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000179 DAOPBQ010000000
VERSION     DAOPBQ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10970_length_6261_cov_4.101354"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKW60_07180"
     CDS             <1..201
                     /locus_tag="PKW60_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPJ99254.1"
                     /translation="EFELTMAITNQKLNPSLETVCLMPNEPHLFLSSSIVREIARFDG
                     DLSGLVPPQIEEAIRKRCSNSV"
     gene            218..1027
                     /locus_tag="PKW60_07185"
     CDS             218..1027
                     /locus_tag="PKW60_07185"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPJ99255.1"
                     /translation="MNSTGLRIIDFHAHAFPDPVAEKALPKLEGASNIKAALDGKVSS
                     LLASMERAGITHSVVASIATKPGQFDAILKWSLGIASDRLIPFPSVHPEDADVPGRVR
                     AIADARFLGIKMHPYYQDFRVDEQRLWPFYKAVRDEGLVLLLHTGFDIAYPHDRIADP
                     VRVARVIGAFPDLKLVTSHLAAWLDWDEAEKHLWGKPVYMDFSYSLHMIDPARARTAL
                     LAHPADYVVFGTDSPWEDQAHAVEAARALDLGPERERLMFYDNAARLLGLN"
     gene            1659..2279
                     /gene="infC"
                     /locus_tag="PKW60_07190"
     CDS             1659..2279
                     /gene="infC"
                     /locus_tag="PKW60_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006693987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPJ99256.1"
                     /translation="MRINERIRARQVRVIDDEGEQLGIMSPEDALREAEERGLDLVEV
                     APNARPPVCRILDFGKYKYQQSKRAKESRKHQHVVTIKEVKYRPKIDKHDFNYKTSHV
                     REFLEDGNKVKLTIMFRGREMTHPEFGRDILARVVEATADLLDELPDASRARLEGRNM
                     ILMLTPGKNAQKKPRQEPRRPVEEKGPEQVVQQAGTDIEADIEPEE"
     gene            2488..2685
                     /gene="rpmI"
                     /locus_tag="PKW60_07195"
     CDS             2488..2685
                     /gene="rpmI"
                     /locus_tag="PKW60_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPJ99257.1"
                     /translation="MPKMKTNKSASKRFKLTKTGKVKRHKAFASHLKTHKSAKRRRSL
                     RKAVILGSEDADGVKRMLPYG"
     gene            2749..3105
                     /gene="rplT"
                     /locus_tag="PKW60_07200"
     CDS             2749..3105
                     /gene="rplT"
                     /locus_tag="PKW60_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPJ99258.1"
                     /translation="MPRSTNSPASRQRRKKFIKLASGYRGSRHRLIKTARQAVEHAGV
                     YAYRDRKVRKRDFRRLWIARINAATRANGIPYSRFILGLRKANIDVNRKMLAEMAVSD
                     EAGFTKLVELAKAELD"
     gene            3117..3845
                     /gene="hisA"
                     /locus_tag="PKW60_07205"
     CDS             3117..3845
                     /gene="hisA"
                     /locus_tag="PKW60_07205"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPJ99259.1"
                     /translation="MRIVPAVDIRDGRCVNLVQGDYGQETVFAEDPVAQARQWFEAVR
                     SLVHIVDLDGAREGRLCTLDALQALNRAGIPYEIGGGIRGLADVEAAVAAGAERVILG
                     TAAYRDRDFLRQAVAAFPEKIAAGIDARDGRVSLSAWRDDTTTSALDFAREMEQAGVV
                     RIIYTDINSDGMMSGPNVEATQAIAQAVAVPVTASGGVASIQDIQRLKEIEPDGVDEV
                     IIGRALYLGAIDIGEAIAVAEGRM"
     gene            3842..4699
                     /locus_tag="PKW60_07210"
     CDS             3842..4699
                     /locus_tag="PKW60_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPJ99260.1"
                     /translation="MTPRYGAVTIIGVGLLGGSLALAMKARNLADTVRGVGRNPKTLD
                     QAIALGIIDTAFQDPAEACQGADLVAVCTPAAGVPAILDAIRPTCDPSTLVTDVASTK
                     AVICAHAARTWPAPSRFVGSHPMAGSEKSGPEFATPSLYEGCVTIVDASPGVADARAQ
                     VTALWEAVGARVVPLEPEHHDALVARTSHIPHIVAGCIARLAAAAGDVRPAVGSGFRD
                     VTRIADGRPELWRDICLTNTAAIAEGLDALMRDLGDVRRMVEEGQAGALEAFFQEARE
                     ARAKALGEP"
     gene            4696..5322
                     /gene="tmk"
                     /locus_tag="PKW60_07215"
     CDS             4696..5322
                     /gene="tmk"
                     /locus_tag="PKW60_07215"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014820890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPJ99261.1"
                     /translation="MKGAFITLEGVEGSGKTTQMALLRDRLEADGHRVLLTREPGGTP
                     IAEAIRGILLDPANTALAPVTELLLYEAARAQHVVERIRPALDEGAIVICDRFADSTT
                     AYQGAGRVLPREAVRELHHLATGALWPDLTIVLDLPVEEGLKRAGAAHTRDRIEREPV
                     EFHERVRQEFLRIAESEPNRVKVVNAAQPVEAVAAAIHVLVRDVVGQP"
     gene            5355..>6261
                     /gene="holB"
                     /locus_tag="PKW60_07220"
     CDS             5355..>6261
                     /gene="holB"
                     /locus_tag="PKW60_07220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HPJ99262.1"
                     /translation="MRLIRRILSRGRIPNALLFWGPGGVGKALTALELAKALNCTELD
                     DDACGACLPCRKVMNGNHPDVKIVTPKAKSRTIPVEDISDVNEICSLRPFESEWRTVI
                     FHDAERMNPPAQNHFLKMLEEPPGRTLFILISEFPRDLLPTIRSRCQLVRFRSLRPET
                     VLDLLRRERDLSPDRAEAIAALSLGRMDRALDLVDTDKRELALQIVAQLRDGGDPVEL
                     AEEFAKNLETQRKQIEDAIDAELKIDRDNAADAQDKQALKELRLAHTAAAIRRAILDY
                     LFLWETWYRDEMIFAATADTSKILNR"
BASE COUNT         1219 a         2025 c         1990 g         1027 t
ORIGIN      
        1 gaattcgagc tgaccatggc gatcacgaac cagaagctga atccgagcct ggagacggtc
       61 tgcctgatgc ccaatgagcc gcatctgttt ttgagttcga gcatcgtgcg cgaaatcgcg
      121 cgtttcgacg gcgatttgag cggcctggtg ccgccacaga tcgaggaggc cattcggaaa
      181 cggtgcagca actctgtgta gggaggcggg acgaagcatg aacagcacgg gactgcgtat
      241 catcgacttc cacgcgcacg catttccaga tccggttgcg gagaaagcgc tgccgaagct
      301 ggaaggagcg tcgaacatta aggcggccct ggacggaaag gtcagttcgc tgctggcgtc
      361 gatggaacgg gcggggatta cacactcggt ggtggcctcg atcgcgacca agccgggcca
      421 gttcgatgcc attttgaagt ggtctctggg gatcgcgtcc gatcggctga taccgtttcc
      481 gtcggtccat ccggaagacg ccgacgtgcc gggccgggtg cgggcgattg ccgatgcgag
      541 attcctgggg atcaaaatgc atccctatta ccaggatttc cgcgtggacg agcagcggtt
      601 gtggccgttt tacaaagccg tgcgcgatga ggggctggtg ttgctgctgc acaccggatt
      661 cgatatcgcg tatccgcacg accggatcgc ggaccctgtg cgtgtagcgc gggtgatcgg
      721 cgcgttcccg gatctgaagc tggtgacctc tcatctggca gcctggctgg actgggacga
      781 ggcggaaaag catctctggg gcaagccggt gtatatggat ttttcataca gcctgcacat
      841 gatcgacccg gcccgcgcac gtaccgcgct gctggctcac cccgcggact atgttgtgtt
      901 cgggacggat tcgccgtggg aagaccaggc gcacgcggtt gaggcggccc gcgcgcttga
      961 cttggggccg gagcgtgaac ggctgatgtt ttacgacaac gcggcgcggc tgttggggct
     1021 gaactgaacc atccaaattg tcgcatccgg gcgttatgtc tcaacaggca gcgtcgcggg
     1081 cgcaggaact gcgtgggctg tgtggaccgc gtggactgcc tcgataccac cgccgattcc
     1141 gcatcttgtg tctctcgctg gctggggggg acgcacgccc agcgtgaccg tcatccggat
     1201 tgcaggggcg gccccctgtg gccgcctgtc tggatagccc cgcgcatccg cctgaaggag
     1261 ggcaggcact ggggcctgcc cctgcaaacg cgatacgcat ggccggaggt gggttgtgcg
     1321 ggcagcgccg ccaggtacgt gctcctgtat ctcccgggca gcaacggaca cgctcgcggc
     1381 gaaattgaca ggcggcaggt gcgtttgata gactcccgct gcattttaaa gtaaagaaga
     1441 agcgccggct tctcaccctg cggcggcttg acgccagcca gcacggttca tgaaccttgg
     1501 gttcctgcgt gatgtgcatc atgccgcggc agagagctgg cgctttctgc cgcgtttttt
     1561 atgggcgcgg atgagccaat ggggcgcctg tccccgggaa caggatgggg tttccccagg
     1621 gaggatgtac tatcgccaag acagatggaa ccggccggat acgcatcaac gagcgtatac
     1681 gcgcccggca ggtgcgcgtg attgatgacg aaggcgagca gctgggcatc atgagtccgg
     1741 aagacgcgct gcgcgaggcg gaagagcggg ggctggacct cgtcgaggtt gctccaaacg
     1801 cccggccgcc tgtgtgccgc atccttgatt tcggcaaata caagtaccag cagagcaagc
     1861 gcgctaagga atccaggaag catcaacatg tcgtgaccat caaagaggtc aagtaccgtc
     1921 cgaagatcga caaacacgac ttcaactaca agacctccca tgtccgcgaa ttcctggagg
     1981 atggaaacaa ggtcaagctc acgatcatgt tccgcggccg ggagatgacg caccctgaat
     2041 tcgggcgcga catcctggcc cgtgtcgttg aagcaacggc cgatctgctg gacgagctgc
     2101 ccgacgccag ccgcgcccgc ctcgaggggc gcaatatgat actgatgttg acgccgggga
     2161 agaacgcaca gaaaaaaccc cgccaggagc cgcgccggcc tgttgaagag aaaggcccgg
     2221 agcaagtggt tcaacaggcg ggtacggata tagaagctga tatcgaaccg gaagaatgag
     2281 cgcggcaatt ggccgcgcgc tggtggtgca actgtctgcc ggtatacaag gtgcgcgtta
     2341 agaccgcacg gcgagaaggg caggccccgc agctcgccac gccgcgtcaa gacgtgcttc
     2401 gtttcggacg gccccggata aggccatttc acaacgaagc ccggtttggc acgacggccc
     2461 gtagaccgga gatgaaggag gaaacgaatg ccaaaaatga aaaccaacaa gtccgcttcc
     2521 aagcggttca agctcaccaa gaccggaaaa gtcaaacggc ataaggcctt tgcgagtcat
     2581 ttgaaaactc acaagtccgc caagcgccgc cgcagcctcc gcaaggccgt cattctgggc
     2641 tctgaagacg ctgacggcgt caagcggatg ctcccgtatg ggtgacgagc ccggagaata
     2701 gaaaacgccc ccgtcctcgg gacggtcaac ttcgtgagga tttgaatcat gccacgttca
     2761 acaaattcgc ccgcgtcgcg ccagcgccgg aagaaattta tcaagcttgc gtccggatac
     2821 cggggcagcc gccaccgcct gatcaagacc gcgcgccagg ccgtcgagca tgccggcgta
     2881 tacgcctatc gcgaccgcaa agtgcgcaaa cgcgatttcc ggcggctgtg gatcgcccgt
     2941 atcaacgcgg ccacgcgcgc caacggcatt ccctacagcc gcttcatcct cggcctgcgc
     3001 aaagccaata tcgacgtcaa ccggaaaatg cttgccgaga tggccgtatc cgatgaagcc
     3061 ggtttcacga agctggtgga attagccaaa gccgaactcg actaggccgg gctgccatgc
     3121 gcattgtacc ggccgtcgat attcgcgatg ggcgctgcgt caatctcgtg cagggcgact
     3181 acgggcagga gaccgttttc gccgaggatc ccgtcgccca ggcccgccag tggtttgaag
     3241 cggtccgcag cctcgtgcac atcgtcgatc tggatggcgc gcgcgaaggc cgcctctgca
     3301 ccctcgacgc cctgcaggcc ctgaaccgcg ccggcatacc ctatgagatc ggcgggggta
     3361 tccgcggcct ggccgatgtc gaggccgccg tggccgccgg cgccgagcgc gtcattctgg
     3421 gcaccgcagc ataccgcgac cgcgacttcc tccggcaggc cgtcgctgcc tttccggaga
     3481 agatcgccgc gggcatcgac gcccgcgacg gccgcgtctc cctcagcgcc tggcgcgatg
     3541 acaccaccac cagcgcgctg gacttcgccc gggaaatgga gcaggccggg gtggttcgca
     3601 tcatctacac cgacatcaac agcgacggca tgatgagcgg ccccaatgtg gaggccaccc
     3661 aggcgattgc acaggctgtc gctgtccccg tgactgcctc cggcggcgtc gcctccatac
     3721 aggacatcca gcgcctgaag gaaattgagc cggacggcgt ggacgaagtc atcatcggcc
     3781 gtgccctgta tctgggcgcc atcgacatcg gcgaagccat cgccgtcgcg gagggaagga
     3841 tgtgacgcca cgctacggcg ccgtaaccat catcggtgtg ggcctcctgg gcggttcgct
     3901 cgccctggcc atgaaggccc gaaacctggc ggataccgtg cgcggcgtgg gacgcaaccc
     3961 caagaccctg gatcaggcca tcgcgctcgg catcatcgat acggcctttc aagaccccgc
     4021 cgaggcatgc cagggggccg atctggtggc cgtgtgcacg cccgcggcgg gtgtgccggc
     4081 catcctcgat gcgatccggc ccacctgtga tccatccaca ctcgttaccg atgtcgccag
     4141 caccaaggca gtcatctgcg cccatgccgc gcgcacctgg cccgccccat cgcgttttgt
     4201 cgggagccat cccatggccg gttccgagaa atccggcccg gagttcgcca ccccgtcgct
     4261 gtacgaaggc tgcgtcacca tcgtcgacgc cagtcccggc gttgcggacg cgcgcgcgca
     4321 ggtcacggcc ctctgggaag cggtcggcgc gcgggtcgtg ccgctcgagc cggagcacca
     4381 cgacgcgctc gtcgcgcgca ccagccacat cccacacatc gttgcgggat gcattgcacg
     4441 gctggcggcc gccgcgggcg atgtccggcc cgccgtcggc tccgggttcc gcgacgtcac
     4501 acgcattgct gatggacgcc ccgaactctg gcgcgacatc tgcctgacca acacggcggc
     4561 catcgcggag ggtctggacg ccctgatgcg cgacttgggc gacgtgcggc gcatggtgga
     4621 agaaggccag gccggcgcgc tcgaagcatt tttccaggaa gcccgggagg cgcgcgcaaa
     4681 agcattggga gaaccatgaa gggtgcgttt ataacgctgg aaggcgtcga gggcagtggc
     4741 aaaaccacgc agatggccct gctccgtgat cgccttgaag cagacggcca ccgcgtcctc
     4801 ctgacccgcg aacccggcgg cacgcccatt gccgaagcca ttcgcggcat cctcctcgat
     4861 ccggcaaaca ccgccctggc gcccgtgacc gaactcctcc tgtacgaggc cgcccgcgca
     4921 cagcatgtcg tcgaacgcat ccggcctgcg ctcgacgagg gcgcgatcgt catctgcgac
     4981 cgcttcgccg acagcaccac cgcctaccag ggcgcggggc gcgtcctgcc acgcgaggcg
     5041 gttcgcgaac tacaccacct ggcaacgggc gccctctggc ccgatctcac cattgtgctc
     5101 gaccttcccg tcgaggaagg cctgaaacgc gccggcgccg cacacacccg cgaccgcata
     5161 gagcgcgaac cggtcgaatt tcacgaacgc gtgcgccagg aatttctgcg catcgcggaa
     5221 tccgaaccca accgcgtgaa ggtcgtcaac gccgctcagc ctgtcgaagc ggtcgccgcg
     5281 gccatccacg tgctggttcg agacgtagtg gggcagccgt gagcctctcg gacatcagag
     5341 atcaggaagt ggccgtgcgc ctcatccggc gcatcctcag ccggggccgc atcccgaatg
     5401 ccctgctctt ctgggggccg ggcggggtcg gcaaggcgct cacggcgctc gagctcgcca
     5461 aagccctcaa ttgcacggaa ctcgacgacg acgcctgcgg cgcctgcctg ccctgccgca
     5521 aagtcatgaa cggaaaccac cccgacgtca agatcgtcac gccaaaggcc aaatcgcgca
     5581 ccatcccggt ggaagacatc agcgacgtca atgagatctg ctccctgcgg ccattcgaat
     5641 cggagtggcg caccgtgatt ttccacgatg ccgagcgcat gaatccccct gcgcaaaacc
     5701 atttcctcaa aatgctcgag gaaccgccgg gacgcaccct gttcatcctg ataagcgaat
     5761 ttccccggga tttgctgccc acgatccgct cccggtgcca gttggtgcgg tttcggagcc
     5821 tccgcccgga gaccgtgctc gatctgctcc ggcgcgaacg cgacctttcc ccggaccgag
     5881 ccgaggccat cgccgcgctc tcgctgggcc gcatggaccg cgccctggac ctcgtggaca
     5941 ccgataaacg cgagcttgcc ctgcagatcg tcgcgcagct gcgcgacggc ggcgatccgg
     6001 tcgagctggc ggaggaattt gccaaaaacc tcgaaaccca gcgcaaacag atcgaggacg
     6061 ccatcgacgc ggagctgaaa atcgaccgcg acaacgcggc cgacgcgcag gacaaacagg
     6121 ctctgaaaga gctgcgcctc gcccataccg ccgcggcaat tcgccgtgct atactggact
     6181 atctgttcct gtgggaaacg tggtaccgcg acgagatgat tttcgcggcc acggcagaca
     6241 caagcaagat cctcaaccgg g
//
LOCUS       DAOPBQ010000180         6245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11013_length_6245_cov_4.249596, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000180 DAOPBQ010000000
VERSION     DAOPBQ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11013_length_6245_cov_4.249596"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="PKW60_07225"
     CDS             <1..1414
                     /locus_tag="PKW60_07225"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPJ99263.1"
                     /translation="SCLWEQFPQACIHAYTVIATQRVRDDIIRSLRLEALSVLIGSFD
                     RPNLTYWFVPRTNLYAQLQAIAARHRGQAGIVYCARRADVEQLSAFLKRAGYRSMPYH
                     AGLGAEQRKRSQERFLREDDAIVAATVAFGMGIDKPDVRFVVHAAMPQSLEHYQQESG
                     RAGRDGFPADCYLLFSEADRELWRGIQGSQEPALQEISDAKLAEMDAVCAGSTCRRRA
                     ILAYFGEQYPAKRCGACDVCLSRARDSALESSSGAILTLLHEIEPLAPPPASEEADWD
                     EVLFEKLRRVRRDEARDRKLPAYRIFGDATLHDMVRKKPVDKAAFARVEGVGRQKCRD
                     YWRVFCTAIREHLGDTDSANTGSRPVASVSAGKRPPSDFQPAGVTAHELFEQETSLED
                     ACRQLGRSERWVVAKLEAFLRDTGRSTPYPWVDDETFARVQEAAGQVIGTRIKSIRQF
                     AGDDLSETEIRLCLACLRDT"
     gene            complement(1427..2668)
                     /locus_tag="PKW60_07230"
     CDS             complement(1427..2668)
                     /locus_tag="PKW60_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPJ99264.1"
                     /translation="MERTTAHSTRQSWSTFKRYRNLLASGTGTLSKRPRFEPEEPCYM
                     DHGRGCRLWDIDGNEYIDFRNGLGPISLGYCYPAVNEAVAAQLEKGTVFSHPCVLEGE
                     VAERVCDMVPCAEKVRYLKTGGEACAAAFKIARAATGRDIIAQCGYNGWVNSLASGAN
                     VLPRVREDAPVGVPVEISRLHRALPWNDLAPYEKLFAEEGGKVAAVAVSMDYATADQG
                     PEFLRALRDLTRKNGALLFLDEIVSGFRVALGGYQEYCDVDCDLAVFAKGISNGLPLS
                     VIAGKASVMDALDRAVVSSTYSGEALSLAAARATLDVYRKHDVIGHLYAMGQRFQEGV
                     NELFARYEYPLEARGLAPCIALTECDRGRTVDNPVDTLFREAYTHGLSLYLVCYINFS
                     HQPEDIDEALARLEDTLKALA"
     gene            complement(2818..4104)
                     /locus_tag="PKW60_07235"
     CDS             complement(2818..4104)
                     /locus_tag="PKW60_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99265.1"
                     /translation="MRSKTRRTGARRAILLLTCAVGILLALIVAVRHHRTEPLIRAES
                     PEMLAKRTSPDNAWYALRDLARSLPQPPPPASPGILTTMRGGLGPAFIATRLAIRLPD
                     DDPKLLDYLEATRQTAETMPQILETQPFYWPPLESLQANRENLAAFEALSRILCGHAL
                     HRARENRPEDAVRFVLTALQLAKSIASDGPFENVLTGCSLISEAAVSAMSLPWSTYPP
                     ELGKDFLARVSALSAGNISLSSALDWELRLAEAGLYTIDSPSEPDGLTGNLLDAALRR
                     FLRKYSKALRESDTTRFADLPSWSSRLERARLLEQKALPTRFTSLAAEAVERRTHART
                     QVEGMTAVVALELYRQNHGRYPEALADLTPDILEAVPIDDYAGEPLRYAIQDHSYLKY
                     STGHVAGDDGGDPKRDLLLPPAIASAFASSLTNPGE"
     gene            complement(4107..5426)
                     /locus_tag="PKW60_07240"
     CDS             complement(4107..5426)
                     /locus_tag="PKW60_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99266.1"
                     /translation="MHGARGHKRRNRREIRTVLVLAALVCVLGAAILLVHSGKSRGLR
                     PPPLAPELQARIESPQNAASILAQAIALQPSNLPPAREYPVPGKPDEMRLFETSKDSI
                     AHALSIWAPDDAPEVAEYIEQCQPAIDKGLEALNAPFFLLPSGQLRDLYPARRILELI
                     RIACRHAWEVREDYDRALDLLFDGVRMCRLLHPGGNASLAVQTSREKIKLWTMLSAYL
                     QQGSNAALLNRAFDEMRQLEEIPLQDYAHIIDAEWRTQYDYMMEGSRFHERGKLPILP
                     GFLFFRWRSRELRTFISYMKAWQTCLALPYLSFEDALDAAPEEMREGITRGGYSPGGW
                     LYEAKFTETIGLASYRQVLLLILLELHRLDTGHYPDTFAEVAAGRSEICLEDPFTGTA
                     FQYHPATPERLVVSPGTGKPPRAFTHLGPQARYVEVVNGKMMVVTEE"
     gene            complement(5428..>6245)
                     /locus_tag="PKW60_07245"
     CDS             complement(5428..>6245)
                     /locus_tag="PKW60_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ99267.1"
                     /translation="YLRFLAMSAHHALVTTLRRKRTILAGVAALLPVVIPLVLTVFME
                     GAFGREGNKIFVMLMEDIYVKALLPLLAMFFGIMLIGEDVESQTIPYLLTRPVPRSAL
                     ILGRFVALLAIAAGLIVPGVFLTFAASTALGNFGFSAANLRLMLHYDGALLMGLLGYG
                     AFCMWLGAFFKRPIIIGVMALFFWQRLALYVPGLVDFFTIEKYVVALLPPLAEAREAP
                     VLRTALAEYQKKMFLVSATKSAIALLCITIALIFLTVLVVRWREYSQARAMES"
BASE COUNT         1171 a         1867 c         1999 g         1208 t
ORIGIN      
        1 gagctgcctg tgggaacaat ttccccaggc ttgcatccat gcctacaccg ttatcgccac
       61 ccagcgcgtg cgagacgata ttattcgttc acttcgtctt gaagcgctgt cggtgctcat
      121 cggctcgttt gatcggccca acctgaccta ctggtttgtg ccgcgcacca acctttatgc
      181 gcagcttcag gccattgcag cgaggcaccg tggccaggcc gggatcgtgt attgcgcccg
      241 gcgggcggat gtcgagcagt tgtccgcatt tttgaagcgt gcgggatacc gcagcatgcc
      301 ctatcacgca gggctgggcg ccgagcagcg taaacgcagc caggagcggt ttctacgcga
      361 ggacgacgcg atcgtggccg ccacggttgc cttcgggatg ggcattgaca agcccgatgt
      421 gcgttttgtg gtgcacgccg ccatgccgca gtcgctggag cactaccaac aggaaagcgg
      481 gcgcgccggg cgcgatgggt ttcccgccga ctgctacctg ctgttcagcg aagccgatcg
      541 cgagctgtgg cgcggcatcc agggcagtca agagccagcg ctccaggaga taagcgacgc
      601 gaaactcgct gaaatggacg ctgtgtgtgc cggcagcacc tgccggaggc gggccatcct
      661 cgcttatttc ggggaacagt atcccgccaa gcgctgcggt gcctgcgatg tctgtctgag
      721 ccgcgccagg gacagcgcgc tggaatcaag ctccggcgcc atcctgacgc tgctgcatga
      781 gattgagccg cttgctccgc cgccggcaag cgaggaggcc gattgggacg aagtcctgtt
      841 cgagaaactt cggcgcgttc ggcgggatga agcccgggac cgcaagctgc cggcctaccg
      901 tatctttggt gatgcgacgc tccatgacat ggtccgcaaa aaaccggtcg acaaggccgc
      961 atttgcgcgg gttgaaggcg tggggaggca gaaatgccgc gactactggc gggttttctg
     1021 cacggcgatt cgcgaacatc tcggcgatac ggactcagcg aacaccggta gccgcccggt
     1081 agcttccgtt tcggcaggca agcggccgcc ttcggatttc cagccggcgg gcgtcaccgc
     1141 acacgaattg tttgagcaag agacctctct cgaagacgcc tgccgtcaac tcgggcgcag
     1201 cgaacggtgg gttgttgcaa aacttgaagc ctttttacgc gataccggcc gttcgacgcc
     1261 ctatccctgg gtcgatgacg agacgtttgc acgcgtgcag gaagccgccg ggcaggtcat
     1321 tggcacgcgg atcaagtcga tccggcaatt tgcgggcgat gacctgagcg aaacggagat
     1381 tcgcctgtgc ctggcctgcc tgcgtgatac gtgaatctgt gtatctctac gccagcgcct
     1441 tgagcgtatc ttccagacgg gcaagcgctt cgtcgatgtc ttccggctgg tgagaaaagt
     1501 tgatgtagca caccagatac agcgagagcc cgtgcgtgta ggcctcgcga aacagcgtat
     1561 cgaccggatt gtccacggtc cttccccggt cgcactcggt cagggcgatg cacggagcca
     1621 ggccgcgcgc ttccaggggg tattcgtacc gtgcgaacaa ctcgttgacg ccctcctgga
     1681 accgttggcc catcgcgtac agatggccga tgacatcgtg tttgcggtag acgtccagcg
     1741 tggcccgggc tgcggccagg gagagggcct cacccgaata ggtcgagctg accaccgccc
     1801 gatcgagcgc gtccatgaca ctggctttcc ccgcaatgac cgaaagcggc aggccgtttg
     1861 atatgccttt ggcaaagacg gccagatcgc aatcgacgtc gcaatactcc tggtatcctc
     1921 ccagggcgac acggaatccg gacacaatct cgtcgagaaa gagcagggcc ccgttctttc
     1981 gcgtgagatc ccgcagcgcg cgcaggaact ctgggccctg atctgcggtg gcgtaatcca
     2041 tggacaccgc gaccgccgct accttgccgc cctcttctgc aaagagcttt tcgtaggggg
     2101 cgagatcgtt ccaggggagg gcgcggtgca gtctggagat ttccacgggc acgcccacgg
     2161 gcgcgtcttc ccggacccgg gggaggacgt tcgccccgga cgccaggctg ttgacccaac
     2221 cgttgtagcc gcactgggcg atgatgtcgc ggccggtagc ggcgcgcgcg atcttgaacg
     2281 ccgctgcgca ggcctcgcct cctgttttca ggtagcgcac tttttccgcg cagggaacca
     2341 tgtcgcaaac gcgttccgcc acttcccctt ccagcacaca cgggtgcgag aatacggtgc
     2401 ccttctccaa ttgcgcggca acggcttcat ttacggccgg gtagcagtat ccgagcgaga
     2461 ttggccccag cccgttgcgg aaatcaatgt attcgttgcc atcgatatcc cagagccggc
     2521 agccgcggcc gtggtccatg tagcagggct cttcgggttc aaagcgcggc cgcttcgaca
     2581 gagtgccggt gcctgatgcc aggaggttcc ggtagcgttt gaaggtggac caggattgcc
     2641 tcgtgctgtg ggcggtcgtg cgttccattg gacattcctc cggagcggtt cgggatgagc
     2701 cgggctgttt acgccaaatc tatcacaccc ttggctgtcc ggccctggcg cttctctttg
     2761 gcggccggca agcttcgttg tctgggcgcg gggaaaaatc ggcgcgcgcc gacaggccta
     2821 ttctccggga ttggtgagcg aggatgcaaa tgcagacgcg atggcgggag gcagcagcag
     2881 gtcccgcttg ggatcgccgc cgtcatctcc ggccacgtgc ccggtgctgt acttcagata
     2941 tgaatggtct tgtatggcat aacgaagagg ctccccggca tagtcatcta tggggacggc
     3001 ttccaggatg tccggcgtga gatcggccag cgcttcgggg tatctgccat gattttgacg
     3061 atacagctcg agcgccacca cggcggtcat gccctcgacc tgtgtccttg cgtgcgttcg
     3121 cctttcaacc gcctcggcgg cgaggctcgt gaaccgtgtc ggcagcgcct tctgctcaag
     3181 gaggcgggcg cgctcgaggc ggcttgacca tgagggcagg tcagcaaatc gggtggtatc
     3241 cgattcgcga agcgctttcg agtatttgcg cagaaaacgc cgcaatgcgg catccagcag
     3301 gttgccggta agtccgtccg gctcgctggg gctgtcaatc gtgtagaggc ccgcctcggc
     3361 caggcgcagt tcccagtcca gcgcgctcga caggctgatg ttgcccgcag ataaggcgct
     3421 cacccgggcg agaaagtcct ttcccagctc cggcggatag gtgctccacg gaaggctcat
     3481 ggcagaaacg gcggcttcgg aaatcaggct gcaccccgtt agtacgtttt cgaaagggcc
     3541 gtcacttgct atggatttgg cgagttgcag tgcagtgagc acgaaccgga cggcgtcttc
     3601 cggcctgttt tctctggccc tgtgcaatgc gtggccgcag agaatccggg acagcgcctc
     3661 gaacgcggcg agattctccc ggttggcttg cagcgattcg agggggggcc aatagaacgg
     3721 ctgtgtttcc agtatttgcg gcatggtttc cgcagtctgg cgtgtggctt caaggtagtc
     3781 gagcagcttg ggatcgtcgt ccggaaggcg gatggcgagg cgtgtcgcaa taaacgctgg
     3841 acccagtccg ccgcgcatgg tcgtcaggat gccggggctt gcggggggcg gcggttgagg
     3901 aagacttcgc gccagatccc ggagcgcata ccaggcatta tccggtgaag tccgtttggc
     3961 aagcatttcc ggcgattccg ccctgatcag cggttccgtg cgatggtggc gcacggcgac
     4021 aattaatgcg agcaggatgc ccacggcgca ggtgagcagc agaattgcgc gccgggcgcc
     4081 ggttcgtcgc gttttgctgc gcacggtcat tcctctgtca cgaccatcat tttgccgttc
     4141 actacttcga cataccgcgc ctgcggacca agatgggtaa acgcccgcgg cggttttccc
     4201 gtcccggggc tgaccaccag gcgctcgggg gttgctggat ggtactggaa cgcagttccg
     4261 gtaaacgggt cttctaagca gatttccgat ctgccagcag ccacttcggc aaatgtgtcc
     4321 ggatagtgtc cggtatccag gcgatgcaat tccagcagaa tgagcagcag cacctggcgg
     4381 tatgatgcca gcccgattgt ctcagtaaac ttggcttcat acagccagcc cccagggctg
     4441 tagccaccgc gcgtgatccc ttccctcatt tcttcgggtg cagcgtccaa cgcgtcttca
     4501 aaagaaagat agggaagcgc caggcaggtc tgccaggctt tcatgtagct gatgaaagtc
     4561 ctgagctccc tgcttctcca gcggaaaaat agaaatccgg gcaggatcgg caatttgccc
     4621 cgctcatgga accggctgcc ctccatcata tagtcgtact gggttcgcca ctcggcgtca
     4681 atgatgtgcg cataatcctg caggggtatc tcttccagct gcctcatctc gtcaaaagcc
     4741 cggttcagga gggctgcatt gctgccctgc tgaaggtagg cgctgagcat ggtccacaac
     4801 ttgatctttt cccggctggt ctgcacggcc agggaggcat ttccacccgg gtgcagcaga
     4861 cggcacatgc ggactccatc gaacagcaga tcaagcgcgc gatcgtagtc ttcacggacc
     4921 tcccacgcgt ggcggcaggc aattcgaatg agttcgagga tcctccgcgc cggatacagg
     4981 tccctgagct ggccgctggg aagcaggaaa aaaggcgcgt tcaaggcttc gaggcctttg
     5041 tcgatcgcgg gctggcattg ttcgatgtat tcggccacct cgggggcgtc atccggcgcc
     5101 catattgaga gtgcgtgtgc gatggagtct tttgaggttt caaacagccg catttcatcg
     5161 ggcttgcctg gaacgggata ttctcgtgca ggtggcaaat tggagggttg cagcgctatg
     5221 gcctgggcca ggatggacgc cgcgttttgc ggcgactcga tgcgggcctg aagctcgggc
     5281 gcaagagggg gcggccgcag tccgcgggat ttgccgctgt gcaccagcag tatggctgcg
     5341 ccgagcacac agaccagcgc agccaggacc aggaccgtgc gtatttcacg acggttcctg
     5401 cgcttgtgcc cacgtgcgcc gtgcacatca actctccatc gctctggcct gcgaatactc
     5461 gcgccagcgg acgaccagca cggtcaggaa aatcagcgcg attgtgatgc aaagcagggc
     5521 aatggcggat tttgtggcgc tgaccaggaa cattttcttc tgatattccg cgagcgcggt
     5581 gcggagcacc ggagcctccc gggcttccgc gagcggcggc aacagcgcaa ccacgtattt
     5641 ttcaatggtg aaaaagtcga cgaggcccgg cacgtagagg gccaggcgct gccagaagaa
     5701 cagcgccatg acgccgatga tgatcggccg cttgaagaag gcgcccagcc acatgcagaa
     5761 cgccccgtag cccagcagac ccatcagcaa tgcgccgtcg taatgcagca tgagccgcag
     5821 gttggcggcg gagaatccga aattccccag cgcggtagat gccgcaaatg tcagaaagac
     5881 ccccggcacg atcaaccccg ccgcgatcgc cagcagcgcc acgaagcggc ccaaaatcag
     5941 cgcagagcgg ggcacgggcc gtgtgaggag gtaggggatg gtctgtgatt ccacatcttc
     6001 cccgataagc atgatcccga agaacatggc cagcaggggc aggagggctt tgacgtagat
     6061 gtcttccatc aacatcacga agatcttgtt tccctcgcgc ccgaacgcgc cttccatgaa
     6121 tacggtgagc accagcggaa tcacaaccgg cagcagggcc gccacgccgg ccaggatggt
     6181 ccgtttccgc cgcaatgtgg tgaccagcgc gtggtgggcc gacatcgcca ggaaacgcag
     6241 gtacg
//
LOCUS       DAOPBQ010000181         6205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11102_length_6205_cov_3.743577, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000181 DAOPBQ010000000
VERSION     DAOPBQ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11102_length_6205_cov_3.743577"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /gene="murD"
                     /locus_tag="PKW60_07250"
     CDS             <1..1068
                     /gene="murD"
                     /locus_tag="PKW60_07250"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPJ99268.1"
                     /translation="IPVMSEMELAWPFTRARILAVTGTNGKTTTTELIRALIESCGHT
                     VLLAGNNDTPLSAAVMVEPAPAYIVLEVSSFQLEFAETFRPWLAALLNVTPDHLDWHG
                     GMDGYTAAKRRLFARQSTGDTAVLNDDDAVAASLDVPPEASVWRFSLERPEDRGLWVK
                     GGGIFHGGRQVARTADTRLPGRHNLQNVLAALCMMRAGQFAWDRVLAGLREFKGVEHR
                     IEFVTEEGGIAFYNDSKSTNIDSLKVALESFERPLVLVAGGRGKGADYRVLRPLVAQH
                     VKVLITLGEDAPLLEAAFGDLVPARRATDMGEAVAAARNAAETGDIVLLSPACASFDM
                     FDNFEQRGRVFKACVRELVKG"
     gene            1073..2212
                     /gene="ftsW"
                     /locus_tag="PKW60_07255"
     CDS             1073..2212
                     /gene="ftsW"
                     /locus_tag="PKW60_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HPJ99269.1"
                     /translation="MKRETLLLLVTVMILSSVGLVLVFSASPAYTSVTGKDESKDMFH
                     FLSRQVIFLALGFAGMLALSRIDYHVWGRRWAYTPVVVISLALLVIVLVIGAEINNSR
                     RWIALPIGTLQPSELAKFALIIFLAAKLAENQRDIASFRRGFIPPVFYIALFAGLVVL
                     EEDLSTPMVIACSGMLLVGMAGARWSHLFLSACGGSAMVVFLCLTSPERVRRLMAFRD
                     PWGDRFNDGMQLVQSLSGFARGGVWGQGVGAGEQKLFYLHAANNDFIFSVWGEEMGLL
                     GTLSLVALFALFLIVGTRVALCAPDLLGALLASGVVAMIAMQAGINMGVTTGLLPTTG
                     LPLPFISAGGSALIVNLAMVGLLLNVAQQASEPEPKGHRLAAARA"
     gene            2240..3364
                     /gene="murG"
                     /locus_tag="PKW60_07260"
     CDS             2240..3364
                     /gene="murG"
                     /locus_tag="PKW60_07260"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPJ99270.1"
                     /translation="MRIMITGGGTGGHTSPAVAVIEELQKRDAMLAVQWIGRHGGVEQ
                     RLCERNGIAFRGVPVEGWPRKRSLRRVWVALKLACSVVRCWLYIGTFRPQAVLGVGGY
                     VSLPLGLAAQRRGVPTVIHEQNRLLGMANRMLAPKAARILLSFPNTMGTYPAERARVV
                     GNPVREGFLHPPPRDEACTALDLDPSIPVVLVCGGSQGAQSLNDALAGALPQFLPGSI
                     QFLWMAGQSGITEARRIAAEHTLRIDTFAFIDDMVTACAAADLVVSRSGASTTAELAC
                     LGKPSILVPFPHATDNHQEQNARAFEEAGAARVILDRELTGDGLAAAIRELLDDDSLR
                     SRMGEAARSLARPGAAETIAEEIFALIFEGVVETASGDEV"
     gene            complement(3400..>6205)
                     /locus_tag="PKW60_07265"
     CDS             complement(3400..>6205)
                     /locus_tag="PKW60_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPJ99271.1"
                     /translation="VLSMPLEATARYVRVTARKTPNVERLLLGEIVLEPPVNEGEALP
                     GRRLPPTPMQVKRTLDQTLLSAGVQFLYGCYPTDILRDPDGEPAGIVVANRSGRQAVL
                     ARVIIDATDRGTVARMAGAKFTGYPAGSHPFTRIVVGGERPADTPARVEPLPSPVQNV
                     QAADGAAMHYEAFAYTLDIPMPDASWASFARAESILRDASYARGQVAASDLPFQIPPD
                     RIFGRSSLDSDWPGAEAIDLNVFRPADVNRLYVLGGCADLPRDAAVRLLRPLAYMDAG
                     TRVGRAAAEEALRLGEPDAPSSRVLIPSVYVPGNTDAASERGDVRETLTGLRPFQDGL
                     PAIPAEAVALPVLGEYDVVVIGGGTGGAPAGISAARQGAKTLVVEYLHGLGGVSTLGL
                     IGNYYWGHRQGFTAEIDAAMEALGAKKGSAGWDVEVRMEWYRRELRAAGADIWFGCIG
                     CGAVVEGSTVKGAVVATPLGRGVVLAHVVIDSTGNADIAVAAGAQSVYTDGSHVAVQG
                     TGMPPRNLGAHYTNTDYTLTDDGDLFDTWRTFVVARGKYEAAFDLAQIVDSRERRRVV
                     GDFVISPLDLFNGRTYPDSIGYSRSNFDSHGFTVHPLFTLDPPHKEGVGGYTPYRALL
                     PRGYDGMLVTGLGISAHRDAIPILRMQPDVQNQGYAAGAAAAMAAKSRSPLRDIDIKA
                     LQRHLVEIGCVPENVLTDTDSYPFTEGELRNAVANVVNDFDGLGIILAQPDEARPFLR
                     SAYELAANPDDPLVYARILGMLGDAMGITTLVEAIDSHDWDEGWNFTGMGQYGNSVSY
                     VDSLIIAAGTSGDQRALEPVLRKLGALNAESEFSHHRAVAIALESLKAPEAAEPLARL
                     LQMPEMTGYAATTIERAQSEVLIADPNISRTLSLRELILARALYRCGDHEGLGERILR
                     TYAQDLRGHYARHAHAVLNE"
BASE COUNT         1086 a         1928 c         2032 g         1159 t
ORIGIN      
        1 atcccggtga tgagcgagat ggagctggcc tggccgttca cccgggcccg catactcgca
       61 gtcacgggca ccaatggaaa aaccacgacg accgagctta tccgggcact tatcgagtcc
      121 tgcggccata cggtgctgct ggcggggaac aacgacacac cgctgtccgc agccgtgatg
      181 gttgagcccg ctcccgcgta catcgtgctc gaggtcagca gcttccagct cgaatttgct
      241 gagacgttcc ggccgtggct cgctgccctg ttgaatgtga cgccggatca cctcgactgg
      301 cacggcggca tggacgggta taccgcggca aagcggcggc tcttcgccag gcaaagcacc
      361 ggtgacacgg cggtattaaa cgacgacgac gcagtcgccg catcgctgga tgtcccgccc
      421 gaggcgtcgg tgtggcggtt cagcctcgaa cgcccggaag atcgcgggct ctgggtcaaa
      481 gggggcggga tcttccatgg tgggcggcaa gtcgcacgaa cggccgatac gagactgccc
      541 gggcggcaca atcttcagaa tgtgctggcc gcgctgtgca tgatgcgcgc gggccagttc
      601 gcctgggatc gggttctggc gggtctgcgc gaattcaagg gggtggagca ccggattgaa
      661 ttcgtgacgg aagagggcgg catagccttc tacaacgact cgaaatcgac aaatatcgac
      721 agcctcaaag tcgccctcga gagcttcgaa cggccgctcg tcctggtggc gggcggacgg
      781 ggcaaaggcg ccgattaccg cgtactgcgg cctctcgttg cgcagcatgt caaggtcctg
      841 atcacgctgg gtgaggacgc ccctctgctg gaagcggcat ttggcgacct ggtgccggcg
      901 cgccgggcca ctgacatggg cgaggccgta gcggcggcgc ggaacgccgc cgagacaggc
      961 gacatcgtct tgctgtcgcc cgcctgcgcc agttttgata tgttcgacaa ttttgaacag
     1021 cggggacgcg ttttcaaggc ctgtgtacga gaacttgtga aaggatagtg cggtgaaacg
     1081 ggaaacgctg ctgttgctgg tgacggtgat gattttgagt tccgtgggcc ttgtgctggt
     1141 gttcagtgcc agcccggcct acacgagcgt caccggcaag gacgaatcca aggacatgtt
     1201 tcactttctt tcacgccagg tcattttcct ggccctgggt ttcgcgggca tgctggcgct
     1261 ttcccggatc gattatcacg tgtggggccg gcgctgggcg tatacgcccg tcgtggtgat
     1321 ttctctggcc ttactggtga tcgtgctcgt catcggagcc gagatcaata attcacgccg
     1381 ctggatcgcc ctgccgattg gtacgctcca gccgtccgag ctcgccaaat tcgcgctgat
     1441 catttttctt gcggccaaac tggcggagaa tcagcgtgat attgcgagtt ttcgccgtgg
     1501 atttattcct ccggtgtttt acatagccct gttcgcgggg ctggtggtgc tcgaggagga
     1561 tctgagcacg cccatggtta tcgcctgttc aggcatgctg ctggtgggca tggcgggcgc
     1621 gcggtggtca catcttttct tgagtgcctg tggcgggagc gccatggtgg ttttcctgtg
     1681 cctgacttcg ccggaacgcg tccggcgcct catggccttt cgcgatccct ggggcgatcg
     1741 gttcaatgat ggcatgcagc tcgtgcagtc gttgtcggga tttgcccgtg gcggcgtctg
     1801 ggggcagggc gtcggcgccg gagagcagaa attgttttat cttcatgcgg caaacaacga
     1861 tttcatcttc tcggtctggg gggaagaaat gggcttgctg ggtacgcttt cgctggtggc
     1921 gctctttgcc ctttttctga tcgtgggtac gcgcgtggcc ctgtgcgcgc cggatcttct
     1981 gggcgctctg ctggcatcgg gcgtggtggc catgatcgcg atgcaggcag gcatcaacat
     2041 gggcgtcacc acggggttgc tgcccaccac cggattgccg ctgccgttca tcagcgcggg
     2101 cggcagcgcg cttattgtca atctggccat ggtaggccta ttgttaaacg tcgcgcagca
     2161 ggcatcagaa ccagaaccca aaggccaccg gctggcggcg gcgcgagcgt aaggcgcgag
     2221 gcaaggcagt agcagtgcta tgcggataat gattacaggc ggcggcacgg gcgggcatac
     2281 gtctccggcc gtggccgtca ttgaagaact tcagaaacgg gacgcaatgc tcgccgttca
     2341 atggatcggc cggcacggcg gagtggaaca acgcctctgc gagcgcaacg gcatcgcatt
     2401 tcgcggcgtg ccggtcgaag gatggccccg gaagcgttct ctgcgccgcg tctgggtcgc
     2461 gttgaaactg gcctgcagcg tggtgcgttg ctggctctat atcgggacat ttcgccctca
     2521 ggcggtgctg ggcgtgggcg gatacgtgtc gctgcccctg ggcctggcgg cacagcggcg
     2581 cggcgtgccg accgtcatcc acgaacaaaa ccggcttctg ggcatggcca accggatgct
     2641 cgcgccaaag gcggcacgga tcctcctgag ctttcccaat acgatgggca cgtatccggc
     2701 agagcgcgcc cgcgtggtcg gcaatcccgt gcgcgaaggc tttctgcatc ccccgccgcg
     2761 agacgaagcc tgcacggcgc ttgacctcga tccctccatc ccggtcgtgc tggtctgtgg
     2821 cggaagtcag ggcgcgcaat cgctcaatga cgccctggcc ggcgcgttgc cgcagttttt
     2881 gccgggctcg attcagtttc tgtggatggc cggacaatcc gggatcacgg aggcccggcg
     2941 catcgcggcc gaacacacgt tgcggatcga cacgtttgcc ttcatcgacg acatggtcac
     3001 ggcctgcgca gccgccgacc tggtcgtgag ccgatcgggt gcatcgacca cggcggagct
     3061 ggcctgtctc ggcaaaccgt cgattctggt gccgtttccg cacgccaccg acaaccacca
     3121 ggagcaaaac gcgcgcgcct tcgaagaagc gggtgcggcg cgagtcatac tcgaccggga
     3181 attgaccggc gacggcctcg cggcagccat ccgcgaactt ctggacgacg acagcctccg
     3241 gagccggatg ggcgaagccg cgcgttctct ggcccggccc ggcgcagcgg agaccatcgc
     3301 cgaagagatc ttcgcgctga tctttgaagg cgtggtcgag acggcgtccg gcgacgaagt
     3361 ctagccgctc tcttcccggc gctggcgccg attcacgaat tattcgttca ataccgcatg
     3421 ggcatggcgc gcatagtgtc cacgaagatc ctgcgcatac gtgcgcagga tgcgttcgcc
     3481 caggccctca tgatcgccgc agcgatacag cgcgcgggca agaatcagct cgcgcagaga
     3541 aagcgtgcgc gagatgttcg ggtccgcgat aagaacctcg ctctgagcgc gttcaatggt
     3601 cgtggccgcg taaccggtca tctccggcat ctgaagcagg cgggccagcg gttctgcggc
     3661 ttcgggggct ttcagggatt ccagcgcgat ggccaccgcc cggtggtggg aaaactcgct
     3721 ttccgcattc aacgcgccca gcttgcgcag cacgggttcc agggcgcgct ggtcaccgct
     3781 ggtccctgcc gcgatgatca ggctgtcgac gtagctgaca ctgttgccgt actgccccat
     3841 gccggtaaaa ttccagcctt cgtcccaatc gtggctgtcg atcgcctcca ccagggttgt
     3901 gatgcccatt gcgtcgccga gcatgcccag gatgcgggcg tagacgagcg gatcgtccgg
     3961 attggccgcc aattcgtatg ccgagcgtaa aaagggccga gcttcgtccg gttgggccag
     4021 aataatgccc agtccgtcga aatcgttcac gacgtttgcc accgcgttgc gcagttcacc
     4081 ttctgtgaag gggtaggaat cggtatccgt gagcacattt tcggggacgc agccaatttc
     4141 aacgagatgc cgctgcagcg ccttgatgtc gatatcgcgc aacggcgagc ggcttttcgc
     4201 ggccatggcg gcggctgccc ccgcggcata gccctggttc tgaacgtcgg gctgcatgcg
     4261 caggatagga atggcgtcgc ggtgggcgct gatgcccagg ccggtgacca gcatgccgtc
     4321 gtatccccgg ggcagcagcg cgcggtatgg cgtatacccg cccacgcctt ccttgtgggg
     4381 aggatccaat gtgaataagg gatgcaccgt gaagccatgg ctgtcgaaat tgcttcgcga
     4441 atacccgatg ctgtccgggt aggtccggcc gttgaagagg tccaggggtg atatgacaaa
     4501 atcgccgacg acgcggcggc gttcacgcga atcgacgatc tgcgccaggt cgaacgcggc
     4561 ttcatacttg cctcttgcaa cgacgaaggt gcgccaggtg tcgaaaaggt ctccgtcgtc
     4621 ggtcagcgtg taatcggtgt tggtgtaatg ggcgcccagg ttgcgcggcg gcatgccggt
     4681 gccctgcacg gcgacgtgcg acccgtccgt atagacgctt tgggctcccg cggcgaccgc
     4741 gatatccgca ttgccggtgg aatcgatgac cacatgcgcc agcactacgc cacgacccaa
     4801 cggcgtagcg acgaccgcgc ctttgacggt gctgccttcg accacggccc cgcagccgat
     4861 gcagccaaac cagatgtcgg cgccggccgc acgcagctcc cggcggtacc attccatacg
     4921 gacctcgaca tcccatccgg cggagccttt tttggcgcca agcgcttcca tggccgcgtc
     4981 aatttcggcg gtaaacccct gccggtggcc ccagtagtaa ttgccaatga ggccgagcgt
     5041 gctgacaccg cccaatccgt gcaagtattc gaccaccagg gtcttggcgc cctgccgggc
     5101 cgcggaaatg cctgcgggcg cccctccggt gccgccgccg atgaccacca cgtcgtattc
     5161 acccagaacc ggcaacgcaa cggcctccgc gggaatggcg ggcagaccat cctgaaaagg
     5221 ccggagaccg gtgagtgttt cgcgcacatc gccccgttca gaggccgcat ccgtgtttcc
     5281 aggcacatag accgatggaa tcaagacacg cgatgaaggc gcatccggct cacccagacg
     5341 gagcgcctcc tcggcggcgg ccctgccgac ccgggtcccg gcgtccatat acgccagagg
     5401 gcgaagcaat ctgacagcgg catcacgcgg caggtcggca caaccgccca gcacgtacag
     5461 ccggttaacg tccgcgggcc gaaacacatt caagtcgatg gcttccgcgc cgggccaatc
     5521 gctatcgagc gaggagcggc caaaaatgcg atcgggcggg atctgaaacg gcagatctga
     5581 ggccgcgacc tgtcctcgcg cataggacgc gtcccgcagg atactttcag cacgggcaaa
     5641 cgacgcccac gatgcatcgg gcatggggat atcgagggta tacgcaaagg cttcgtagtg
     5701 catggccgcc ccgtcggccg cctgcacgtt ctggacgggc gaagggagcg gttccacgcg
     5761 cgccggagtg tccgccggtc tctctccccc cacgacgatc cgtgtaaacg gatgagaacc
     5821 cgcagggtac ccggtaaatt tcgcccccgc catgcgcgcg accgtacccc ggtcggtggc
     5881 gtcgatgatc acccgggcca gcaccgcctg gcgccccgag cggttggcca cgacaatgcc
     5941 cgcgggctct ccatcaggat cacgcaaaat atcggtcgga taacagccat acaaaaactg
     6001 gacgcccgcg ctgaggagcg tctgatccag cgtgcgtttg acctgcatgg gcgtcggcgg
     6061 caggcgccga cccggcaggg cttcgccttc gttcaccggc ggctcgagca cgatttcgcc
     6121 cagcaacagg cgctcgacgt tcggcgtttt gcgcgcggtg acgcgcacat aacgcgccgt
     6181 cgcttccagc ggcatgctca gcacg
//
LOCUS       DAOPBQ010000182         6153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11220_length_6153_cov_3.778452, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000182 DAOPBQ010000000
VERSION     DAOPBQ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11220_length_6153_cov_3.778452"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PKW60_07270"
     CDS             <1..481
                     /locus_tag="PKW60_07270"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HPJ99272.1"
                     /translation="PGETAVSKPEELVTLLGFDSVTSYVWIHHVPLPQFPQTPYTYVR
                     DKYVEHWNEAAPRYPVPYYPNVTMGWDSSPRCHLDNEFTYSGYPFMATISENDPKAFE
                     EGLRAIKALADTLPPSQRIVTINCWNEWTEGSYLEPDARNGMAYLEAVRRVFKDSAP"
     gene            complement(543..1616)
                     /locus_tag="PKW60_07275"
     CDS             complement(543..1616)
                     /locus_tag="PKW60_07275"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPJ99273.1"
                     /translation="MRHHFIAEYKPLPAYGLWILLALALTAVPLAAAQPPASGPLVCD
                     APVYDFGERDNFTRVNHQFQLKNAGSEPLNIDRVLTTCGCTVANLKQRTLAPGESVPL
                     EVEVSFEGRTGAFEKHLFVLYGGKTQNRVQLTMKGMAVQHLRITPPTVNLSNSAMDHA
                     SESSTTIESLEEASAFKVREVKTDSPYLELTAETSPDGLKHTIHVRTRPPLPLGVSPA
                     QIHVFTDSDQYPVLDIPVALRAVDKIEVVPKEVVVYDKGTGDADTAIRFLNVIPGSVS
                     QFTVTGVETPGESIVSEVIPRPENRYLIKLQNLPVDPAFEGKDIVILTDIPGRERVPV
                     PLRIKKYPAPRIGLSPKPPASNP"
     gene            1734..3758
                     /gene="ppk1"
                     /locus_tag="PKW60_07280"
     CDS             1734..3758
                     /gene="ppk1"
                     /locus_tag="PKW60_07280"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPJ99274.1"
                     /translation="MQNVHQIREVSTLSFNERVLQEAEDRRNPSMERLKFLGIFSSNM
                     DEFFKVRVASVQRRIELGHSGMQDVLEVLAHKARELDERFRAAYGDIASELASQGIRI
                     LNEHDIEHCAQELGPWLDDYFRENVLPSLVPIILRKSQPFPQLTDGSLYFGVKMWGDP
                     VRYAILEIPPEHPRFVELPNGAIMYVDDLIRHALNDIFYIFDYERIGAYEFKISRDAQ
                     LDIDNDFSEGHVRKMERVLKQRKAGRPTRLVHDATMPMELLQLLQDRMKITKYDTLIA
                     GARYHNMKDLISFPNHRKDVSFEEMEPAPHPILDHGRQPMTKIIQEQDLLITYPYQSF
                     DNVIRLLREAAIDPEVQEIKMTLYRVARHSHVVNALINAARNGKKVFVSIELQARFDE
                     KNNIKISEALTESGATVVYGVPPMKVHCKLLLIKGKKSHLAGFSTGNFNEITARQYVD
                     TVLLTSDKRLVDEADLVFDYLDSASKMRAVTTPKFKHLLVSPFNTRKTLMKLLNREKK
                     KGDQGYVFLKVNHLTDERIIEGLRETAEAGVKMDFVVRTTYAMRPHPNIRAISILDRF
                     LEHQRIYVFGQGDDRAVFMSSADLMERNIDWRVEVAFPIFDPLLQREILDTCELQVND
                     SYKARILDEIQSNPYVNSGTQGLRAQYATHAYYQRLGSQAFSVSTPAEGI"
     gene            3891..4880
                     /locus_tag="PKW60_07285"
     CDS             3891..4880
                     /locus_tag="PKW60_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPJ99275.1"
                     /translation="MLSRDRVIRVLQHERPDRTPIYAWVKFTLGPQIEDLFGSVEAFE
                     DHYEFDFAHIFGGPSPFPESAVVALREANGGRIGLPAALDLPLNDPGAMEDYRGIIDE
                     IRHHKIERGRFVYVQTPGNFEIVSNLVTLETHLRGLLEYPEELRELYRRLTEWSCAFA
                     RHCLELGIDMIFVADDWGAQNTLLFSESIWREAIYPSHRTLVEAVRRAGGYIGLHSDG
                     NVEAVVDGIVELGYDLVHPWQESAGMNLASQKKRYGGQFVVMGGLDVQSTLGFGNLRR
                     VESEIERVLKLFPDGGLLFCTSHFVQDHCSIEELIFAYDVVYRRVRELGRGGR"
     gene            4943..>6153
                     /locus_tag="PKW60_07290"
     CDS             4943..>6153
                     /locus_tag="PKW60_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99276.1"
                     /translation="MILAIWCAIQLGAVELPVGSAPEPVTFDHFPTRLHACVWRNWTL
                     VPMDTLASVCGAETAELLDMGRSMGLEGPPEITPNQQRRSYITVIRRNWHLMPYEQLL
                     SLLGWTAEEMAYTLREDDFLYVKLGNLKPQCEPVRYEERTPQVREAEARLAGWVKEAF
                     PGGAGVPRDPLFAFVARLSEMPAKRTEGSRESQFNPRYAPSYFALYGDPLLEDDPDQG
                     SFPSGYLARLAESGVNGVWMQAVLYKLTPFPWDPSLSEHYEKRLENLKALVARAKGLG
                     IGIYLYLNEPRAMPLAFYDEHPDLKGAVEGDHAALCTSAPEVLEYIRDAVERLAREVP
                     DLAGFFTISASENLTNCWSHFRGNDCVRCSKRAPDEVIAEFHRAIYEGIQRGGASTRL
                     FAWDWGWQDAWV"
BASE COUNT         1273 a         1798 c         1860 g         1222 t
ORIGIN      
        1 gccgggggag accgcggtgt ccaagcccga agagctcgtg acgctgctgg gtttcgacag
       61 tgtcacgtcg tatgtctgga tccaccacgt gccgctgccg caatttcctc agacgccgta
      121 cacgtacgta cgggacaaat acgtcgagca ttggaacgaa gccgccccgc ggtatcccgt
      181 gccctattat cccaacgtga ccatgggctg ggactcgtcg ccccgctgcc atttggacaa
      241 tgagttcacg tactccgggt acccattcat ggcgacgatc tcggagaacg accccaaggc
      301 cttcgaagaa ggactgcgtg cgatcaaagc gctcgcggat acgctgcccc catcgcaacg
      361 catcgtcacc attaattgct ggaacgaatg gaccgaaggc agctatctcg aaccggacgc
      421 ccgcaacggc atggcctatc tggaagcggt gcggcgggta ttcaaagaca gcgccccgta
      481 aaccgtcctg cctcctttcc gtgaatgaat gggctgcttt gggctaaacg caggctggcg
      541 cgtcagggat ttgacgcggg cggcttggga gagagcccga tacgtggagc gggatacttc
      601 ttgatgcgca gcggcacggg aacccgttcc cggccgggaa tatcggtcaa aatcacgata
      661 tctttgccct cgaaggccgg atccacggga agattctgca gcttgatgag atagcggttc
      721 tccgggcggg gaatgacctc cgagacgatg ctttcacccg gtgtttcgac tccagtcacc
      781 gtgaattggg acacgctgcc gggtattacg ttcaagaacc ggattgcggt gtcggcatcg
      841 ccggtccctt tgtcataaac cacgacttct ttgggcacca cctcgatctt gtcgaccgca
      901 cggagcgcta ccgggatatc cagtacggga tactggtcgc tgtccgtaaa cacatgaatt
      961 tgagccggag agacgcccag aggaagtggc ggcctggtgc gcacatgaat ggtgtgtttc
     1021 agtccatccg ggctggtctc ggcagtcagc tcgaggtacg ggctgtctgt cttgacctcg
     1081 cgcaccttga atgcggatgc ctcctccagc gactcgatgg ttgtgctgct ctcgctggcg
     1141 tggtccatcg cggaattgct caaattgact gtcggcgggg tgattcggag gtgttgcacc
     1201 gccattccct tcatggtgag ctggaccctg ttttgggttt tcccgccgta caggacgaaa
     1261 agatgctttt cgaacgcgcc cgtgcgtcct tcaaatgaca cttcgacctc caggggaacg
     1321 gattcccccg gagcgagtgt tctctgctta agatttgcga cggtgcatcc gcaggtggtc
     1381 aggacgcgat cgatattcag cggctcggaa cccgcgtttt tcaattgaaa ctggtgattc
     1441 acccgggtga aattgtcgcg ttcgccaaaa tcatagaccg gcgcatcaca caccagcggg
     1501 ccggacgccg gcggctgcgc agcggccaga ggaactgccg taagggccag ggccagcagt
     1561 atccacagcc catacgccgg taaaggcttg tattcagcaa taaaatggtg tctcataaag
     1621 tcctccgtca accggatcgg gctgccgtgg cgcttatcat agcaacctga gagcggagca
     1681 ttcatccagc cgggacccgg tagctccagt gggcccactg gacaggaaaa ggcatgcaaa
     1741 acgtccatca gattcgcgag gtctcaacgc tctccttcaa cgagcgggtg cttcaggaag
     1801 ccgaggaccg gcgcaatccg tccatggaac gtctgaagtt tctgggcatc ttctcctcca
     1861 atatggacga attcttcaaa gttcgtgttg ccagcgtgca gcgccggatc gagctggggc
     1921 acagcggcat gcaggacgtc ctcgaggtcc tggcgcacaa agcacgggaa ctggacgaac
     1981 ggttccgcgc cgcctacggg gatatcgcgt ccgagcttgc atcgcagggg atccggatcc
     2041 tcaatgaaca cgacattgaa cactgcgccc aggaactcgg tccctggctg gacgactatt
     2101 tcagagaaaa cgtgttgccg agcctcgtgc cgatcattct ccgcaaatcg cagccatttc
     2161 cccagctcac ggacggttcc ctctatttcg gagtgaagat gtggggcgac ccggtgcgct
     2221 atgcgattct cgaaatcccg cccgagcacc cgcgctttgt ggaactgccc aacggcgcga
     2281 tcatgtacgt ggacgacctc atccggcatg cgctgaacga catcttctac atcttcgact
     2341 acgaacgcat cggcgcttat gaattcaaga tctcccgtga cgcccagctg gatatcgaca
     2401 acgatttctc tgaaggccac gtgcgcaaga tggagcgcgt gctcaaacag cggaaggcgg
     2461 gccgccccac ccgcctggtg cacgatgcca ccatgccgat ggagctgctg caattgctgc
     2521 aggacaggat gaaaatcacc aaatacgaca cgctgatcgc cggggcgcgc taccacaaca
     2581 tgaaggatct catcagtttc cccaatcacc gcaaagacgt gtcgtttgaa gagatggaac
     2641 cggccccaca cccgatcctc gaccacggcc ggcaacccat gaccaaaatc attcaggaac
     2701 aggacctgct gataacgtac ccctaccagt cgttcgacaa tgtaattcgc ctgttgcgcg
     2761 aggcggccat cgatccggaa gtccaggaga tcaagatgac cctgtaccgc gtggcgaggc
     2821 attcgcacgt ggtcaatgcg ctgatcaatg cggcacgcaa cggcaagaag gtgttcgtct
     2881 ccattgaact gcaggcgcgg ttcgacgaga agaacaacat caagatctcc gaagctttga
     2941 cggaatcggg ggcaaccgta gtctatggcg tgccgcccat gaaggtgcac tgcaagctgc
     3001 tgctgatcaa aggcaagaaa agccacctgg cgggcttctc cacggggaac ttcaacgaaa
     3061 ttaccgcccg gcaatacgtc gacaccgtcc tcctcacctc agacaagcgg ctcgtggacg
     3121 aggcagacct cgtctttgat tacctcgact ccgcatcgaa gatgcgggcg gtcacgacgc
     3181 cgaaattcaa gcacctgctc gtgtccccgt ttaacacgcg caaaacgctc atgaaactgc
     3241 tgaaccggga gaagaagaaa ggcgaccagg gctacgtgtt tctgaaggtg aaccatctca
     3301 cagacgaaag aatcatagaa gggctgcggg agacggcgga ggcgggagtc aagatggatt
     3361 ttgtggtgcg caccacctac gccatgcggc cacatcccaa tatccgggcc atttccatat
     3421 tggaccgatt cctggagcat cagcggattt acgtgttcgg ccagggggac gatcgggccg
     3481 tgttcatgag ttccgccgac ctgatggagc gcaacatcga ctggcgcgtc gaggtggcct
     3541 ttcccatctt cgacccactc ctccaacgcg agatcctgga cacctgcgaa ctccaggtga
     3601 acgattccta caaagcgcgc attctcgacg aaattcaatc caatccttac gtcaattccg
     3661 gcacccaggg tctgcgcgcc caatacgcga cacacgccta ttaccagcgc ctgggctccc
     3721 aggcattctc cgtctcgaca cccgccgaag ggatatgaag cctcctgccc ccgcgagtac
     3781 cagccgggcg gttgtggaat tttggatgga cggggtagga ttgtcgcggc ggaggcgggg
     3841 tccgccaatt ccgcagcttg cagcgcgctt gagtccaggg gaatttccag atgctctccc
     3901 gcgaccgcgt cattcgggtg cttcagcatg agagacctga tcgaacgccc atctatgcgt
     3961 gggtgaagtt tactttgggt ccgcagatcg aggacctgtt cgggtcggtt gaagcgtttg
     4021 aggatcatta cgagtttgat tttgcgcata ttttcggggg gccgtcgcct tttccggaat
     4081 ctgcggtagt ggcattgcgc gaagcgaacg gcgggcgcat cggcctcccg gcggccctgg
     4141 atttgccgct gaatgatccc ggggccatgg aagattaccg cgggatcatc gatgaaatcc
     4201 ggcatcacaa gatcgaacgc ggacgctttg tctatgtgca gaccccaggc aactttgaga
     4261 tcgtgagcaa tcttgtcact ctggaaactc atctccgcgg cctgcttgaa tatccagagg
     4321 aactgcgcga gctgtaccgg cgtctgacgg aatggagctg tgcattcgcc cggcattgtc
     4381 ttgagctcgg gatagacatg atcttcgttg ccgatgactg gggcgcgcag aatacgctgc
     4441 tgttcagcga atcgatctgg cgggaggcga tctacccgtc ccatcggaca ctggtggagg
     4501 cggtacggcg agccggcggc tatatcggac tccacagcga cggaaatgtg gaggccgtcg
     4561 tggatgggat cgtcgaactg ggctacgacc tggtgcatcc gtggcaggaa tcggcgggga
     4621 tgaatctggc aagccagaaa aagcggtatg gcggccaatt cgtggtgatg ggcgggctcg
     4681 acgtgcaatc cacgctcgga tttggcaatc taaggcgggt ggaatccgag atcgagcggg
     4741 tgttgaagct gttcccggat ggcgggctgc tgttttgcac ctcgcatttc gtgcaggatc
     4801 actgctccat cgaggagctc atttttgcgt atgatgtggt gtaccgccgg gtgcgcgagc
     4861 tcggccgcgg cgggcggtga gtgagttgac agcgtgtgcc gggagcaacc cccggcaggt
     4921 acaaatccgg aaggagcagg caatgatatt ggccatttgg tgcgcgatac agttgggcgc
     4981 cgttgagctt cccgtggggt ccgcgccgga acccgtcaca ttcgaccatt ttccgacgcg
     5041 gctgcacgca tgcgtgtggc gcaactggac gctggttccg atggacacac tggcgagcgt
     5101 gtgcggcgcg gaaaccgccg aattgctgga tatgggccgg tccatgggcc tcgaagggcc
     5161 gccggaaatc acgccaaacc agcagcgccg ctcctacatt accgtcattc gccgaaactg
     5221 gcatctcatg ccctatgaac agctcctcag cctgctcgga tggaccgccg aggagatggc
     5281 ctacacactg cgcgaggatg attttctgta cgtcaagctc ggcaacctga aaccgcaatg
     5341 cgagccagtg cgttatgaag aacgtacgcc acaggtgcgt gaagccgaag cgcggcttgc
     5401 cggctgggtg aaagaggcgt tccccggcgg cgcgggagtt cccagggatc cattgttcgc
     5461 cttcgtcgcg cggctttcgg aaatgcccgc gaaacgcacg gaaggttcgc gggagagcca
     5521 gttcaatccg cgctatgccc cgtcctattt cgccctttac ggcgacccgc tgctggagga
     5581 cgatcccgac cagggctctt tcccgagcgg ttatctggcg cgcctggccg aatccggcgt
     5641 gaatggcgtc tggatgcagg cggtcctgta taaactcacg ccgttcccct gggacccctc
     5701 gctgagcgag cattatgaaa agcgcctgga aaatctgaaa gcccttgtgg cccgggccaa
     5761 ggggctgggg atcggcatct acctctacct gaacgagccg cgcgccatgc ctttggcgtt
     5821 ctatgacgag cacccggatc tgaagggcgc ggtggaaggc gatcatgcgg ccctctgcac
     5881 gagtgcaccg gaggttctcg agtacatccg ggatgcggtg gaacggctcg cccgggaagt
     5941 gccggatctc gcgggatttt tcaccatttc ggcctctgaa aatctgacca actgctggtc
     6001 ccacttccgc ggcaacgact gtgtccgttg cagcaaacgc gcgccggacg aggtcatcgc
     6061 ggaatttcac cgcgccatct acgaaggcat ccagcgcggc ggcgcaagta cccgcctttt
     6121 tgcgtgggac tggggctggc aggatgcctg ggt
//
LOCUS       DAOPBQ010000183         6105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11338_length_6105_cov_4.173719, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000183 DAOPBQ010000000
VERSION     DAOPBQ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11338_length_6105_cov_4.173719"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PKW60_07295"
     CDS             <1..660
                     /locus_tag="PKW60_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99277.1"
                     /translation="WADNADWLRLAFHACAEFPDRPYQHCTPERLAADYDLVAGEIKR
                     FAGKRSLSPSTVIHWGMVHPSAWKVLTERGVKVLSGMFYPNMGSAYTTEGESFPIEET
                     GAPFGYDVNYSLDNARSEYLSRHDALKDFESGLLFSKMDIVCNNVPEDQIVATLEPLA
                     HDANTAEVMDLFTHEQYFWPFYSAHRPDHFQRLETAIRFCTEYGYEPVFFHEGLLGGK
                     E"
     gene            748..4008
                     /locus_tag="PKW60_07300"
     CDS             748..4008
                     /locus_tag="PKW60_07300"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HPJ99278.1"
                     /translation="MSTKPLSSRRLSRVPVWALCAWCCAAAVVAGASRAETVFVEAED
                     FVPTSEGWRAVSNHETRAASRIRTLYGAQGPADATAARTTVLHEAGLYRIWVRYLNVV
                     DRRGPFSVMVSAGAKEIASKTFDLETISEVSNWEYTWQFLEAELPADSVTLTLAKQEP
                     VASSPYVRHVDCLLLTTDPELVPDHVPYGPQTLMRIRLAEGYDRPVYVLLFVDHYRAP
                     WYAHFALGLDGIREGLAVPAEQMLGPGQSTPWCNLSPTIYQDSGAALNLSIRHSYYEL
                     AATFRGTVEFAYTDSASDDKIKPVKTFGIDATPNGLVIVVPPDLTTPEHVALLKRDRD
                     FAEETGRLADAFDWPDYGKPPEAFPFLVTANIGGYGLAVDAAVTAREQKTLDYFGFNG
                     SGERVLHGLWFMKDGSYCRPDLDAMRKRANENAADFRESGRNLEQIAVCMLMDEPTGQ
                     PAAFCAQDEAYRVKFREWLQGQGQTPEQLLVESWEHVRPVEETERADFPALHYFTQRF
                     RTRALGDFMATQRRIIEEAYGRSFPTLVNFSDGAIYYGNFCGQGVDYFELLDSGQQNA
                     IWGEDWANISSTYQCAAFNVALMQAAARKRGQLVGHYLIAHAGRTAWDTKTKAAGETA
                     RGVRIWKNFSYGPAWGTHEGGPPWRTHVWYAKPDVWRANAEITRELGAVEDWLTTAAP
                     APARVALLYSSSSDIWTMWDNLAFGFDRMHTWLALAHAQIPVDILSERDVADGDLAGY
                     TVCYVSGPNLTRAASAQLRAWVESGGTLWLTAGAATRDEYNRPLDELAPILPAQRGTT
                     ETLEAFHYSGRLLHHLNAIDAVTWGSEELEVLSVKEPLTPQSGAEVRATFRDGAAAVV
                     RSGAGKGRVMQTGFLPALCYIKEALNARRAAEKSRDEAVAVAQTRPPEDGQPEATAAS
                     LAVALTTGGAAAPSSVERELLDRSYNPWDFPAAIREALLTPIRDADIRPRLTCDTPLV
                     DAVELPCVQGILVVLANHTLRPLEHLNLEFLMDRSIAYVESVRYGRLTFESPRPGTIR
                     WTMPLDASDYVKLGFEGISGTSAPSEPGSRIAAGRPFSENSGALRGCPQRMRLNVE"
     gene            4117..5352
                     /locus_tag="PKW60_07305"
     CDS             4117..5352
                     /locus_tag="PKW60_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HPJ99279.1"
                     /translation="MRKMLLLILAGIFLGNVALAGDSPQFRGPHRDGRFDEEGLLKTW
                     PEDGPPLLWVAHGLGKGYSSPSVADGKIYVPGMADETTAAIFVLSTEGVIERTILVGP
                     ETEDKQAPGPRSTPTIEGDSLYMISGLGEVYCLDIASGTKRWSVNILERFGGPNIMYT
                     LAESLLVDGDRVICTPGGPDAGLAALNKHTGETIWTTHGFSDPASYCSPIIYPHNGRR
                     LLLTETANFLVGVDADSGQFLWQVEHLTRDGIHAVTPLYLNGLVYYTGGYKSGGGAVR
                     LSDDGSAVEPVWKDETLDCQHHGVVLDQGYLYGTSHMFGNELICTEMATGKVMWTTKE
                     VRQGVVIYADGMLYIYEGPARGIVHLVKATSEGLQRGGMFTVTEGDGKHWAHPAIANG
                     VLYIRHGDALLAWNLRPSP"
     gene            complement(5467..6027)
                     /locus_tag="PKW60_07310"
     CDS             complement(5467..6027)
                     /locus_tag="PKW60_07310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPJ99280.1"
                     /translation="MEKTFDIVAIIGSLSKNSLNRKVAQALAELAPAGFNISIAEIAQ
                     LPMYNQDLDENPPDEWTAFRERIRAADAVLFVTAEHNRSVPASLKNALDIGSRPYGQN
                     VWDAKPGAIVSASPSAVGGFGANHHLRQSLVFLNVPTLAQPEAYIGGADKLFDESGRL
                     VNDSTRKFLQRFVQAYAAWVTMHVKS"
BASE COUNT         1188 a         1890 c         1841 g         1186 t
ORIGIN      
        1 tgggccgaca acgcggactg gcttcggctg gcgttccacg catgtgcgga atttcctgac
       61 cggccgtatc agcattgcac accggagaga ctcgccgccg attacgacct cgttgccgga
      121 gaaatcaagc gattcgcagg aaaacgcagc ctttccccct ccaccgtcat tcattggggc
      181 atggttcatc cttcagcctg gaaagtcctg actgagcggg gcgtgaaagt cctcagcggg
      241 atgttctatc ccaacatggg cagtgcctat accaccgagg gcgaatcgtt cccgatcgag
      301 gaaaccggcg ccccatttgg ctacgacgtc aattacagcc tggacaacgc ccgctcggag
      361 tatctgagcc ggcatgatgc gttgaaagat ttcgaaagcg gcctcctctt ttcgaagatg
      421 gacatcgtgt gcaacaacgt gcctgaggac cagatcgtgg ccacgctgga accgcttgcc
      481 cacgacgcaa atacggctga agtcatggat ctgttcacgc atgagcagta tttctggccg
      541 ttctacagcg cccaccggcc ggaccatttc caacggctcg agactgctat ccgcttttgc
      601 actgaatacg gctatgaacc ggtcttcttc cacgagggcc tgcttggcgg aaaagaataa
      661 aacgaagcct gctgtgccgt gaaagcccgc gccgcggcca tggatacctc gaaacggaaa
      721 gggtttccac cggatatgga tgcatgtgtg tccacaaaac cattgtcgtc aagacgattg
      781 tctcgcgttc cggtgtgggc tctctgcgcc tggtgttgcg ccgcggccgt ggtggccggc
      841 gcctcccggg ccgagacggt atttgttgaa gccgaggatt tcgtcccgac atccgagggg
      901 tggcgggcgg tgtccaacca tgagacccgg gccgcgtctc gtatccggac cctgtacggt
      961 gcgcaggggc ctgcggatgc caccgcggcc aggaccaccg tgttgcatga ggcgggactg
     1021 taccgcatct gggtgcgtta tctgaacgtg gtggatcggc gtggaccatt cagcgtcatg
     1081 gtgtccgcgg gcgcgaagga aattgcgtcg aaaaccttcg atttggaaac aatttccgag
     1141 gtttcgaact gggaatacac ctggcaattc ctggaggcag agcttcctgc ggactccgtc
     1201 acgctgacgc tggcaaaaca ggagccggtc gcctcgtcgc catatgtgcg ccacgtagac
     1261 tgcctcctgc tcacgacgga tccggaactc gtgccggacc acgtcccgta tggcccacaa
     1321 accctgatgc gcatccggct tgccgaggga tacgaccggc cggtctatgt gctcctgttc
     1381 gtcgatcatt atcgcgcacc gtggtacgca catttcgctt tgggattgga cgggatacgc
     1441 gaagggttag cggtgcccgc ggagcagatg cttgggcccg ggcagtcgac gccgtggtgc
     1501 aatctctccc ccaccatcta tcaagacagc ggtgcggcgt tgaatttgtc aatccgtcac
     1561 agctactacg aactagcagc aacttttcgc ggaaccgtgg aattcgcgta cacggactcg
     1621 gcgagcgacg acaagatcaa gccggtgaag acttttggca tcgacgccac gcccaacggc
     1681 ctggtgatcg tcgtaccgcc tgatctcacc accccggaac atgtcgccct gttgaaacgc
     1741 gatcgtgatt ttgccgaaga aaccgggcgg ctggccgatg cgttcgactg gcctgactac
     1801 ggtaaaccgc ccgaggcgtt tccgtttctc gttacggcca atattggcgg atacggcctg
     1861 gccgtggatg ccgcggtgac cgcccgcgaa cagaagacgc tcgactattt cggattcaac
     1921 ggctccggcg aacgcgtcct tcacgggctg tggtttatga aggatggctc gtattgccgc
     1981 cccgatctgg atgccatgcg aaagcgggcg aatgagaatg ccgcagattt tcgcgaatcg
     2041 ggcagaaacc tggagcagat cgcggtatgt atgttgatgg acgagccgac aggtcaaccc
     2101 gcggccttct gcgcacagga tgaggcctat cgcgtcaagt ttcgcgaatg gctccagggg
     2161 caggggcaaa ctccggagca gctcctggtc gagagctggg aacacgtccg cccggtggag
     2221 gaaaccgaac gcgcggactt ccccgccctg cactacttca cgcagcggtt tcgcacgcgc
     2281 gcgctcggcg atttcatggc cacccagcgc cgcatcattg aagaggccta tggccgctcg
     2341 ttcccgaccc ttgtcaattt cagcgatggc gccatctatt acggcaattt ctgtggacag
     2401 ggcgtcgact actttgaatt actcgattcc ggccagcaaa acgcgatctg gggggaagac
     2461 tgggccaata tctcctcgac ctaccagtgc gccgcattca acgtggcgct catgcaggcc
     2521 gcggcccgta agcggggcca gctggtcggc cattatctga tcgcccacgc cggccgcacc
     2581 gcgtgggaca caaaaaccaa agcggccggt gagacggccc gcggcgtccg catctggaag
     2641 aatttcagct atggacccgc ctggggcact catgaaggcg gcccgccgtg gcgcacgcac
     2701 gtctggtacg caaaaccgga cgtgtggagg gccaacgccg aaatcacccg ggagcttggc
     2761 gccgtcgagg actggctgac aacggcagcc cccgctcccg ccagggtggc cctcctctac
     2821 agctcgtcct ccgacatctg gacgatgtgg gacaatctcg cgttcggttt tgaccgcatg
     2881 catacctggc tggcgctcgc ccacgcacaa attccggtag acatcctgtc cgagcgcgac
     2941 gtcgcggatg gcgacctcgc cgggtatacg gtgtgctacg tgtccggacc caatctgacg
     3001 cgcgcggctt ccgcccaact gcgcgcctgg gtcgagtcag gcggcacgct ctggctcacg
     3061 gcgggcgcgg caacccggga tgaatacaat cgtccgctgg acgaactcgc ccccatactc
     3121 ccggctcaac ggggaacgac cgaaacactc gaggcttttc actattcggg gcgcctgttg
     3181 catcacctca atgcgataga cgctgtgacg tggggaagtg aagagctgga ggtgttgtcc
     3241 gtcaaagagc ccctcacacc gcagtccggc gctgaggtac gggcaacgtt ccgtgatggc
     3301 gcagctgctg ttgtacgtag cggcgcaggc aaaggccggg tgatgcagac gggcttcctg
     3361 ccggcgctgt gctacatcaa agaagcgctg aacgcgcgcc gcgccgccga gaagagccgg
     3421 gatgaggcgg ttgccgttgc gcagacccgc ccgcccgagg acgggcagcc cgaagccacg
     3481 gctgcgtctc tggcggtcgc tctaactacc ggcggcgctg cagctccgtc aagcgttgaa
     3541 cgggagctgc tcgatcgctc gtacaacccc tgggattttc ctgcagccat ccgcgaagcg
     3601 cttttgacac cgatccggga cgcagacatc cgcccgcggc tcacatgcga cacaccgctg
     3661 gtcgatgccg tcgaattgcc ctgcgttcag ggaatcctgg tcgtgctggc caaccacaca
     3721 ctgcgcccgc tcgagcacct gaacctggaa tttctgatgg atcgctctat cgcatatgtg
     3781 gaatccgttc gttatggccg gctgacgttt gaatcgcccc gacccggcac gatccggtgg
     3841 accatgccgc tcgacgccag cgattacgtc aagctgggat ttgaaggaat ctcaggaacg
     3901 tcggcgccat ctgaacccgg ttctcgtatt gctgcagggc ggccattttc cgagaattct
     3961 ggagcactcc gtggttgccc tcaacgaatg cgcttaaacg tagaatgaac actgcctgct
     4021 gttgcttcct ccgcctctga acggtattct cgtggacatc gtgccgctgg ctgaggcaac
     4081 ggctcaatgg ccttatggaa tacgaggtaa aaacgaatgc ggaagatgct gctcctgatc
     4141 ctggctggta tttttctggg gaacgtggct ctggcggggg attcgcctca gtttcgcggg
     4201 ccccatcgcg atgggcgctt cgacgaggag gggctgctga aaacctggcc agaggacggc
     4261 ccgccgctcc tctgggtggc acacgggctc ggtaaaggct attcatcccc ctccgtggca
     4321 gatggaaaaa tctatgtgcc gggcatggcc gacgagacga ccgcggccat cttcgtgtta
     4381 tccaccgaag gcgttatcga gcgcacaatt ctggtgggcc ctgaaacgga agacaaacag
     4441 gccccggggc cgcgctcaac accgaccatc gagggcgaca gcctctacat gatttccgga
     4501 ctgggcgaag tctactgtct cgacatcgcc tcgggcacga aacgctggag cgtgaacatt
     4561 ctcgaacgct tcggcggccc caacatcatg tatacgctcg cggagtccct gctggtggat
     4621 ggcgaccgcg ttatctgcac ccccggcggg ccggatgccg gcctggccgc gctcaacaag
     4681 cacaccggcg agacaatctg gaccacccac ggattcagcg atcccgcatc ctattgctcg
     4741 cccatcatct atccgcacaa cggccggcgc ctgctgctga ccgaaacggc caacttcctc
     4801 gttggcgtcg acgcggacag cgggcaattc ctctggcagg tcgaacatct tacccgcgac
     4861 ggcatccacg cggtgactcc cctctacctc aacgggctcg tgtactacac cgggggctac
     4921 aaatccggtg gcggagcggt gcgcctctcc gacgacggct cagcggtgga gcccgtctgg
     4981 aaagacgaga cactcgattg ccagcatcac ggcgtcgttc tcgaccaggg ttacctctac
     5041 gggacgagcc acatgttcgg caatgaatta atctgcactg aaatggccac tggaaaggtc
     5101 atgtggacta cgaaggaagt gcgccagggc gtcgtcatct acgccgatgg catgctctac
     5161 atctacgaag gccccgcgcg cggcatcgtg cacctcgtca aagccacttc cgagggactc
     5221 cagcgtggag gcatgtttac cgtgaccgag ggcgatggca agcactgggc acaccccgcc
     5281 atcgccaacg gcgtcctgta tatccggcat ggcgacgcgc ttctggcctg gaaccttcgt
     5341 cccagtccat aatcgactca tcatacgctc gagcggcagt gctccagctg ctttgcggca
     5401 caaagcgctc cgaagggcca cgccgggctg gcggcagatg taaaagttcg gctcgcgtgc
     5461 cgcccgttac gacttcacgt gcatggtgac ccacgccgcg taggcctgca cgaatctctg
     5521 aagaaatttt cgcgtgctgt cattaacgag cctgccgctc tcatcaaaca gcttgtcagc
     5581 accgccaata tatgcttccg gttgggcgag agtgggcacg ttcaagaaga cgagcgactg
     5641 gcgcaggtgg tgattcgcgc caaatccgcc caccgcgctg ggggatgcgc tgacgatggc
     5701 gccgggcttg gcatcccaga cgttctggcc atacggccgc gacccgatat ccagggcatt
     5761 cttcaaggat gccggtaccg agcggttgtg ctcggctgtg acaaagagga cggcgtcggc
     5821 cgcacggatc cgctcacgaa atgccgtcca ttcgtctggc ggattttcgt ccaggtcctg
     5881 gttgtacatg ggcaactgcg cgatttcggc gatgctgata ttgaatccag caggagcaag
     5941 ctccgcgagc gcctgggcca ctttgcgatt gagtgaattt ttactgagac tgccgatgat
     6001 cgcgacgatg tcgaaggttt tttccatgtg atgtctccct tgcggcccgc ctgtatgcct
     6061 cactgccgtt aaatgctgaa gccccccatt ccatgttagg caagt
//
LOCUS       DAOPBQ010000184         6093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11366_length_6093_cov_3.331567, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000184 DAOPBQ010000000
VERSION     DAOPBQ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11366_length_6093_cov_3.331567"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="PKW60_07315"
     CDS             complement(<1..1626)
                     /locus_tag="PKW60_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPJ99281.1"
                     /translation="MAIDSMLKMVILCPTGDGKRILRTLHGMGIAEMTDVQERSEDIG
                     EHLAPPETSTEEIDQVLQKIHLLLGLIDTLAPEKKSFVAGLAPVPLVIEPGELDAVRQ
                     NFDLESHYIRAHELDAAYRRADRHSGEIQNQLEELRPFESLAIHVGDLAKTRHTRVLF
                     GEILPADLTALQLDANAPGTLTVERLYEGKYYREGAPKLEQGTDPPRKPVQVLAAFVL
                     ADEQKAREALARHGFTEVSLPDVPGTIRDRIRELTLDQASLEEEVAAIRAGARDLARH
                     RREILILKAFWDSCKNLALARLKCARGRWVQVMTGFIREKDAGTLKEALKGYDRAEVI
                     LTKPSPDEVVPISISLPKSVRPVRMLVDLFGLPMYGAFDPSPFVLFGFFLFFGMCFGD
                     VGYGLMLLGISAYIMLRTKAYEGLYNFGKLLLMASIPTILFGFIFGSCFGDLYTAAYL
                     GEGNPVERLLNATTLVNPVENPVVVLLMALLIGVLNQFYAIGLKVYSHVKQGDKATAV
                     YDGVLWLIALPGFILLVCRMFATLPGWLNALGLV"
     gene            complement(1626..1940)
                     /locus_tag="PKW60_07320"
     CDS             complement(1626..1940)
                     /locus_tag="PKW60_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99282.1"
                     /translation="MKTLIDKVLSLENEAERILDSARQEAKAIDENADAQIARIQLED
                     EAALLRRIDEYRNTATQKHQDEAAQARAALVQEIERVKRVPQSLLDEQAAKIVARFCE
                     FE"
     gene            complement(2340..4028)
                     /locus_tag="PKW60_07325"
     CDS             complement(2340..4028)
                     /locus_tag="PKW60_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPJ99283.1"
                     /translation="MAIWPCFVTLAIVLCAWSGFAVAAEPAAQWPLITDGQDISGHER
                     HLENHGVQFGASSPDGASAARFDGRGAWLALPEALAPRFGTGDFTFSLWVNTEEVLDD
                     NLGDLLSKYDAASRTGLNLSILTCSGVSNSQPNFRTVHFGIDQRRTDPQWTDCGRPGN
                     AVLIFGMAVHEGDLYAATCEPETGESGRVYRYAGGTNWIDCGSPDASNAVAALAVCDG
                     QLYAGSSWYDTTGSALAESPNTTPGGKVYRYEGGTQWTYCGTLSNPETGEAGTMGGLG
                     VYRGKLHATTLKQDGFGLYRYEGGTEWTYCGNPGRRVLNPGVFDGALYMVSYDAPGGP
                     FRYDGSAWEYVGGTIDPPIHQDYSFTVYDGHLHVSTWPNANVYRMDSNGVWSLLGRPG
                     DELETMAMMVYNGSLYTGTLPSSKVYRYDGGTLWTPVGQQLDTAEGRYRRAWSMAIYQ
                     GKLFCGTLPSGKVFCMEAGRNVTWDHALQPGWRHVAAVRDAAQLRLYIDGRPVAVSAT
                     FDPDHFDLANGEPLRLGAGSGDFFNGWMRDVRLYENALSEQDVLELHRAGQSAE"
     gene            complement(4418..>6093)
                     /locus_tag="PKW60_07330"
     CDS             complement(4418..>6093)
                     /locus_tag="PKW60_07330"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPJ99284.1"
                     /translation="EWTLYFRNTGEADSPILSDVLAIDTSFERVSPEEYVLHRNKGDN
                     CTADSFEPVDEMLAPGMDRRIVNTGGRPTQSEFPYFNISSGGEGLIFVVSWAGQWSAQ
                     FTRDDGNRLRLRAGQEVTHFRLHPGEEVRTPMVVLQFWNGDRLHAQNVWRAWMLAHNL
                     PRPGGKLPPLPQLAACSSHQFDEMIHANTENQLFFIDKYLERGIALDYWWMDAGWYYN
                     KTGWPHTGTWEVDLNRFPGGLRPISDHAHAKDVNIIVWFEPERVAADTWLTENHPEWI
                     FGGANGGLLNLGNPDARAWLIGHVDRLLTEQGIDLYRQDFNMDPLPFWRANDAEDRQG
                     ITEIRHVEGYLAYWDELIRRHPALLIDSCASGGRRNDLETLRRAVPLLRSDYIMEPIG
                     NQCHTYALASWFPFYGTGTSKTSTYLVRSTLCPHFTACWDQRDNGLDWVNLKRLMDQW
                     KAFAPNYFGDYYPLTPYTLDDSEWIAWQFHRPADGQGMVQAFRRQNSIYLTAQLPLHA
                     LDPDARYVVVDVDQPEALTEFSGSELRSPGIPVTMREKPSAVVYTYEKR"
BASE COUNT         1242 a         1898 c         1765 g         1188 t
ORIGIN      
        1 gaccaggccg agcgcattca gccagccggg cagcgttgcg aacatccggc agacgaggag
       61 aatgaagccc ggcagcgcaa tgagccaaag cacgccgtcg tacacggccg tggccttgtc
      121 tccctgtttc acatggctgt agactttcaa gccaatggca taaaactggt tgagcacgcc
      181 gatcagcagc gccatcagca gcaccaccac ggggttctcg acggggttca ccagcgtagt
      241 cgcgttcagg agccgctcga cggggttgcc ctctcccagg tacgcggccg tgtacaaatc
      301 gccaaaacac gaaccaaaaa tgaacccgaa gaggatagtc ggtatgctcg ccatcagaag
      361 cagtttgccg aagttgtaca ggccttcgta ggctttggtg cgcagcatga tataagcact
      421 gattccgagc agcattaacc cgtagcccac atcgccaaag cacatcccga aaaagagaaa
      481 aaaaccaaag agcacgaacg gcgaaggatc aaacgccccg tacatcggga gcccgaacag
      541 atcgaccagc atacggacag gccgaacgct tttcggcagc gaaatgctga tcggcaccac
      601 ttcatccggc gatggtttcg tcaagatcac ctcagcccgg tcgtatcctt tcagcgcctc
      661 tttcagcgtg ccggcatctt tctcccggat aaaaccggtc atcacctgca cccatcggcc
      721 acgggcgcat ttgagacggg ccagtgcaag attcttgcag ctgtcccaga aggctttgag
      781 aatgaggatt tcccggcggt gccgggccaa atcccgtgct cccgcccgga tggccgcgac
      841 ctcttcttcg agcgacgcct ggtcaagcgt caactcgcgg atccgatcgc gaatggtacc
      901 cggcacatcc ggcagcgaca cttcagtaaa cccatggcgc gcgagcgctt cccgggcctt
      961 ctgctcgtcc gccaggacaa aggccgccag cacctgcacc ggtttcctgg gggggtccgt
     1021 cccctgctcc agtttcggag cgccttcccg gtagtacttc ccttcataga ggcgctcaac
     1081 cgtcagagtg ccaggcgcat tggcgtccag ttgaagggcg gttaagtccg cgggcagtat
     1141 ctccccgaac agaacgcgtg tatggcgtgt tttcgccaga tcgccgacgt ggatcgccag
     1201 cgattcaaaa ggcctcaatt cctcaagctg gttctggatc tctccggagt ggcgatccgc
     1261 gcgccggtaa gcggcgtcaa gttcatgggc gcggatgtag tggctttcca aatcgaaatt
     1321 ctgacggacg gcatccagct cgccgggctc aatgacgagc ggtaccgggg cgaggccagc
     1381 cacaaagctc tttttctcgg gcgccagggt atcgatcagg ccgagaagaa ggtgaatttt
     1441 ctgaagaacc tggtcgattt cctcggtcga cgtctcgggc ggcgccaggt gctcaccgat
     1501 gtcttcactt cgttcctgga catcggtcat ctcggcaatg cccatgccat gcaaggtgcg
     1561 gagaatgcgc ttcccgtcgc cggtagggca caagatgacc atcttcagca tggaatcaat
     1621 tgccattact caaactcgca aaaccgggcg acgattttcg ctgcctgttc atcgagcagg
     1681 ctttgcggaa cccgcttgac gcgctcgatc tcctgcacga gcgcagcccg ggcctgggcc
     1741 gcttcatcct ggtgtttctg tgtggccgta ttccggtatt catcaatgcg gcgcagcagg
     1801 gccgcttcgt cttccagttg aatccgggcg atctgagcat cggcgttttc atctatcgcc
     1861 tttgcttcct ggcgggcgct gtcgagaatg cgttccgcct cattttccag ggaaaggact
     1921 ttatcgatca gagttttcat actggcctct caagcgggaa catgcgctgg ggctccagct
     1981 gtgaagttta ccccgcagga aatgatacac gtccggggaa acggatgcaa acaaaacgta
     2041 gaacttccag tataaagtta gcacatttgc ccggatgttt catctattca ccgagcggac
     2101 atgaaatccc cggtacttgc tttcccttcc tggaagcgcc ggcaaacggc gggagggcgc
     2161 cgtactgggg attgaattat gcctcgtgtt tcacggaatg aacgcaagtt tataccagcg
     2221 gataacaccg atgcaatctt cgcagcacca gaaggccgcg aagacggcac catcggccga
     2281 gcgagcaagg cacggcgcgc cgaatcgcag cgccgcccgg gcgggcccga ttcagcgtct
     2341 tattccgcgc tctggccggc tcggtgaagt tcaaggacgt cctgttccga cagggcgttc
     2401 tcgtaaagcc gcacgtcgcg catccaccca ttgaagaaat cgcccgaacc ggcgccaagc
     2461 cgcagcggct cgccgttggc caggtcaaag tgatccggat caaaggtggc gctcaccgcc
     2521 acagggcgcc catcgatgta cagccgcagc tgcgctgcgt cgcgcacggc ggctacatga
     2581 cgccaaccgg gttgcagcgc gtgatcccac gtaacgttcc ggcccgcttc catgcagaac
     2641 accttacccg acggaagcgt cccgcaaaac agcttgccct ggtagatggc catagaccac
     2701 gcccgccggt acctgccttc cgccgtatcc agctgctgac ccacgggtgt ccacagcgtg
     2761 ccgccgtcgt accggtatac cttcgaagaa ggcagagttc ccgtgtagag cgagccgttg
     2821 tagaccatca tggccatggt ttccagctcg tcgccaggcc gaccgagcaa agaccagacg
     2881 ccattggaat ccatgcggta gacattggcg ttgggccatg tcgatacatg caggtgcccg
     2941 tcgtagaccg tgaacgagta atcttgatgg atgggcgggt caattgttcc gccgacgtac
     3001 tcccatgcgc tgccgtcata ccggaacggt ccgccggggg cgtcgtacga caccatatag
     3061 agcgccccgt cgaagacgcc gggattaagc acgcgccttc ccggatttcc gcaataggtc
     3121 cattccgttc cgccttcgta gcgatacagg ccgaacccat cctgtttcag agtcgtcgca
     3181 tgcagcttgc cgcggtagac gcccaggccg cccatcgtac cggcctcgcc ggtctcggga
     3241 ttggacagag tcccacagta agtccactgt gtaccgccct cgtaacgata gactttgccg
     3301 ccgggggtgg tgttaggcga ctcggctaac gcggagccgg tggtgtcgta ccaggaactg
     3361 cccgcgtaga gttggccgtc acatactgcg agcgcagcga cggcattgct ggcgtcggga
     3421 ctgccgcagt cgatccaatt cgtgccgccc gcataccggt acacgcggcc cgactccccc
     3481 gtctccggct cgcaggtggc cgcatacaga tctccctcgt ggacggccat cccgaagatc
     3541 agcactgcat tgccgggccg tccgcagtct gtccactgag gatccgtacg cctctgatcg
     3601 atcccgaaat gcacggttcg gaagttgggc tgcgaattgg agacgcctga acaggtgagg
     3661 atgctgaggt tgagccctgt gcgcgaagcc gcgtcgtatt tgctgagcaa atcaccgagg
     3721 ttgtcgtcca ggacctcttc cgtgttcacc caaagcgaga aggtgaaatc gccggtaccg
     3781 aaacggggcg cgagtgcctc aggcaacgcc agccaggcgc cccggccgtc aaaccgggcc
     3841 gcggaagctc catccggaga agatgcgcca aattggacgc catgattctc aagatgacgc
     3901 tcatgtccgg agatgtcctg gccgtccgtt atgagcggcc attgggcagc cggttccgca
     3961 gccactgcga aaccgctcca cgcgcagagc acaatcgcca acgtcacaaa acaaggccag
     4021 atcgccattg aaagtctcct tttttgaaca ggccacaacg ccgccggccg cattgatcca
     4081 tctcgccgcg cactggtgca cacccatggt cagtcgaaca caggattccg ccttagcccc
     4141 ggatcattcc ccggctactt tggacaggaa cggaactccc catgcggcac catgcatcca
     4201 gcatggcatc cttcggccat tatagctgac gccgcgctcc accgcacaag accatccgcc
     4261 cggccgccca tcccagcgca tccaccctga agaagggcag gcacaggggt ctgcccctgc
     4321 gcgctgccgt aatttggggg ctgttctcgt aaaaaatata ctcgggaagc cgttgtcccg
     4381 tctgtcccgc ggaccgccgg gtcgatggct gccgggttca gcgtttttcg taggtataaa
     4441 ccaccgctga cggtttctcg cgcatcgtca ccggaatgcc gggcgaccgc agctcgctcc
     4501 cggaaaattc ggttaaggct tcgggctgat cgacatcgac cacaacatac cgggcgtcgg
     4561 gatcaagcgc atgcagcggc agctgcgccg tcaggtagat gctgttctga cgtcgaaacg
     4621 cctggaccat gccctggcca tcggcgggcc gatgaaactg ccaggcgatc cactccgaat
     4681 cgtccagggt atacggagtc aacgggtagt agtcgccgaa ataatttggt gcaaaggctt
     4741 tccactggtc catgagacgt ttgaggttga cccagtccag gccgttatcc cgttgatccc
     4801 agcaggcggt gaaatgaggg cacaaggtgc ttcggacgag ataggtgctg gttttcgagg
     4861 tgcccgtgcc gtagaacgga aaccacgagg ccagggcgta cgtgtgacac tggttgccga
     4921 tgggttccat gatgtagtcg ctgcgcagga ggggaaccgc tctgcgaagg gtttcgagat
     4981 cattgcgacg gccgccgctc gcgcacgaat cgatcaacag ggcggggtga cggcgtataa
     5041 gttcatccca ataggccaga tatccttcca catgccggat ttctgtgatg ccctgcctgt
     5101 cctccgcgtc gtttgcccgc cagaacggca gcgggtccat attgaaatcc tgccggtaca
     5161 agtcaatccc ctgctcggtc agcaggcggt cgacgtgtcc gatcagccac gccctggcgt
     5221 ccgggttgcc cagattcagc agaccgccgt ttgccccgcc gaaaatccat tccgggtggt
     5281 tttccgttaa ccacgtatcg gcggcgacgc gctccggctc gaaccagaca atgatgttca
     5341 cgtccttcgc atgggcatgg tcgctgatgg ggcgcaggcc gccgggaaag cggttcagat
     5401 cgacctccca ggtgccggta tgcggccagc cggtcttgtt gtaataccag ccggcgtcca
     5461 tccaccaata atcgagtgca atgcctcgtt ccagatactt atcgataaag aagagctggt
     5521 tttccgtgtt ggcgtggatc atctcgtcga attggtggga actgcacgcc gccagctgcg
     5581 gcaggggcgg aagtttgccg cccggccgcg gcagattgtg cgcgagcatc cacgcgcgcc
     5641 agacattctg tgcatgcagg cgatcaccgt tccagaactg gagaacgacc atcggcgtgc
     5701 gcacttcttc ccccgggtgc agtctgaaat gcgtcacttc ctggccggcg cgcagacgca
     5761 ggcggttgcc gtcatcccgc gtgaactgcg ccgaccactg gcccgcccag ctgaccacaa
     5821 aaatcaggcc ttcgccgccg gacgagatat tgaaataggg aaactcgctt tgtgtcggcc
     5881 gtccaccggt attcacaata cgccggtcca tccctggagc cagcatttca tcaactggtt
     5941 caaagctgtc ggcagtgcaa ttgtctcctt tgttccggtg cagtacgtat tcttccggcg
     6001 aaacccgttc aaatgatgtg tcgatggcca ggacatcgct cagaattggg gaatcggcct
     6061 cgccggtatt ccgaaagtac agcgtccatt cga
//
LOCUS       DAOPBQ010000185         6075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11406_length_6075_cov_4.162625, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000185 DAOPBQ010000000
VERSION     DAOPBQ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11406_length_6075_cov_4.162625"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="PKW60_07335"
     CDS             complement(<1..1111)
                     /locus_tag="PKW60_07335"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002520942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPJ99285.1"
                     /translation="MSCTVLSDLDIAQTAPLKRIGDVAAACGIQDDELEPYGRYKAKI
                     RLEALERLRGNPDGKYIDVTAITPTPLGEGKTVTTIGLSLGLNKLGKRSFACLRQPSL
                     GPTFGIKGGAAGGGHAQIVPMEEFNLHLTGDIHAVGVAHNLLAAMIDNHLHQGNALGI
                     NPHAVTWRRVVDVSDRALRNIVIGLGGKAHGVPRESGFDIAVASELMALLALAEGLHD
                     LRKRIGRVVLGQDRRGAPLTAEDLQAAGAMTALLREALMPTLMQTIENTPVLVHAGPF
                     ANIAHGNSSILADRLALKLSEYVVTESGFGVDMGAEKFVDIKCRYSGLRPDAMVVVAT
                     IRALKFHGGDFSIRPGKPLDPGLHEENLDAVRRGAA"
     gene            complement(1279..2352)
                     /locus_tag="PKW60_07340"
     CDS             complement(1279..2352)
                     /locus_tag="PKW60_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPJ99286.1"
                     /translation="MIKTVTVLALGLAPLIAAAQSENIETYRVIGPEFPGQYKHPASI
                     TQLDCGDLYLAYYGGSGEYEDDTAVYGLRQKRGETTWSDPVVIADTPDRGEGNPVVWQ
                     GPHGLVWLFYVNRYGDTWSNARVKAKISTDGAVTWSDSFMLTFEEGTMVRGRPELLRN
                     GDFLLPMYLETGEDREEIASTTTSFFLRYNHEAKTWTETNRIRSERGNLQAQVAQLDD
                     QYLVAYIRRGGGYERDEIGYVQRSESRDGGFTWSEAKDTEFPNPNAAVDFLRLKNGHL
                     LLVYNNSMNERSPLTVAISMDNDQSYPCRRDIGGGDNTFAYPYAIQTDDEKIHIIYTT
                     NTRTTIMHAVFEEAAITARNWKP"
     gene            complement(2449..3303)
                     /locus_tag="PKW60_07345"
     CDS             complement(2449..3303)
                     /locus_tag="PKW60_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015781732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPJ99287.1"
                     /translation="MPLTYKTETGYTYFEEGSGSVIILLHGLMGGPENFQSVLTDLRK
                     DYRAVAPVLPLFDLPLRKTGVEELSQFLHEFITHKRFHKVALLGNSLGGHVALRYALT
                     HPENVSGMILTGSSGLKENTMGTTLPRRKDYDYVREKAALTFYDPDMATKELVDEVFE
                     VLNSNVKALKLLRMAKSAVRENLADELRHLTMPVGLIWGRDDVVTPPEVAHEFHDKLA
                     LSRLSFIDKCGHAPMMERPDEFNVILRWYLYWIDQVSRGLRSTASGHPLEASAAPGSK
                     EISAVAWA"
     gene            complement(3400..5208)
                     /locus_tag="PKW60_07350"
     CDS             complement(3400..5208)
                     /locus_tag="PKW60_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99288.1"
                     /translation="MPTRESSAVRIFCLCGQKMRLTETMYGRRARCIACQLKIRLPRF
                     EEIPRGVADIFLRDHPEFIREPPQTADPAVEEQDARKRRRTSISVPIDASDTLRKLCS
                     LEKKVSREIKAADGQSKSELEEYKARLKALRAEFNDELHQRLMEASIELTAAQEKLAE
                     LALEARVGELDYRMYSERAGRIRWRRECLERRQINLRAWRVVRDADLAGGYIDLPLSS
                     MDELSMRVPLIEEAAEPSVLLRWMVDQLRAAFGNRKQAEINLEAASRLQEQTGDAGKE
                     GKRAAKVQKAARARAAATIRFWQFRLERFRNDIQSDQRVIDSQLELARGRHQLGELGR
                     DQFDSLESELRQARTDLEKALQTIDRAIAAPSDRDLPALRGTFVERLGKPRPARTRPV
                     TVFAILGLVFMAAIVAGVIFGVWHGLIPMPWLRPGIFGPRPAHITSDAANETGQHSGS
                     PQITIAAPAQETEAALGNDPLSEDGTAAPGAPAAGTDPSDPESLDDAVLAFEDASGEP
                     AGGASAPKRNTVEVELRGVVTSADKAPRFFIRIHTPDGKMQERTRELGGSVAEGWIIR
                     EYNPLQQNITLFNGEDLLILRRGEKAPLPPDRGGAD"
     gene            5936..>6075
                     /locus_tag="PKW60_07355"
     CDS             5936..>6075
                     /locus_tag="PKW60_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HPJ99289.1"
                     /translation="MTIHPTAIIDPKAELATDVEVLPYSIIGPGVTIGSGTVVGPHCV
                     IDG"
BASE COUNT         1132 a         1794 c         1866 g         1283 t
ORIGIN      
        1 tcgctgcgcc ccggcggacg gcgtccaggt tttcttcgtg caatcccgga tcgaggggtt
       61 ttccggggcg aatcgaaaag tcgccgccgt ggaacttgag cgcgcgaatg gtcgcaacca
      121 caaccatggc atcgggccgg agcccgctgt accggcactt gatatcgacg aacttctcgg
      181 cgcccatgtc gacgccaaac ccgctctcgg tcaccacata ctcgctgagt ttgagcgcga
      241 ggcgatcagc aagaatcgag ctgttgccgt gggcgatatt ggcgaacggt cccgcgtgga
      301 cgaggacggg cgtgttttcg atggtctgca tgagcgtggg catcagcgcc tcgcgcagca
      361 gggccgtcat cgcaccggcg gcctggaggt cttccgcggt cagcggagcg ccccgccggt
      421 cttgcccaag cactaccctg ccgatgcgct tgcgcaggtc gtgcaaccct tcagcaagcg
      481 ccagcagcgc catcaattcg ctggccaccg cgatgtcgaa cccggattcg cgcgggacgc
      541 catgcgcctt tccgccaagg cctatgacga tgttgcgcag cgcgcgatca gagacgtcga
      601 cgacgcgccg ccaggtcacg gcatgtggat tgatgccgag ggcgttcccc tggtgcaggt
      661 ggttgtcgat catcgcggcg agcagattgt gagcgacgcc gaccgcgtgg atgtcgccgg
      721 tgagatggag gttgaattcc tccatgggca cgatctgggc atggccgccg cccgcggcgc
      781 cgcccttgat gccgaacgtg ggcccgagcg aaggctggcg cagacaggcg aaagagcgtt
      841 tcccgagttt attcaggccc aggctgaggc cgatcgtggt gacggttttt ccttcgccga
      901 gcggcgtggg agtgattgcc gtgacatcga tatatttgcc atcagggttt ccccgcaggc
      961 gctcgagggc ttccagccgg atcttggctt tgtagcgccc gtaaggctcc aactcgtcgt
     1021 cttgaatgcc gcaggccgcc gccacgtcgc caatccgttt taacggggct gtctgcgcga
     1081 tgtccagatc gcttaacacg gtacaggata tccttatgca cggtcgtgcg gtcgaatcag
     1141 ggtgaacatg ccgcttgcgg aggacgaata ctcccgccat gaggccgtat catagccgag
     1201 cctctaaaaa gcggtcaaac ccggtgcgat tgagtccggt tcagatcgtt ttgaacgaag
     1261 cagctgctca gtggactgtc agggcttcca gttgcgcgcc gtgatcgccg cttcctcaaa
     1321 caccgcgtgc atgatggtcg tgcgggtgtt tgtggtgtaa atgatgtgga tcttctcgtc
     1381 atccgtctgg atcgcatacg gatacgcaaa ggtgttgtcg ccgccgccaa tatcacgccg
     1441 gcagggatag gactggtcgt tgtccatcga gatggccacg gtcaatggcg agcgctcgtt
     1501 catggaattg ttgtacacga gcaggagatg accgtttttg aggcgcagaa aatcgaccgc
     1561 ggcgttcggg ttggggaatt ccgtgtcttt agcctcactc caggtgaacc cgccgtcgcg
     1621 cgactcgctc cgctgcacat agccgatctc gtcgcgctca tatccgccgc ctcggcggat
     1681 gtacgccacc aggtactggt cgtcgagctg cgccacctga gcctgaagat tgccgcgctc
     1741 tgaccggata cggttggttt cggtccaggt tttggcttcg tggttgtagc gcaggaagaa
     1801 gctcgtggtc gtggaggcga tctcttcccg gtcctcgccc gtctcgaggt acatgggcag
     1861 cagaaaatcg ccattgcgga ggagttcggg ccggccgcgc accatggtgc cttcctcaaa
     1921 ggtaagcatg aacgaatccg accacgtgac ggcgccatcg gtcgagatct tcgctttcac
     1981 acgcgcgttg ctccacgtgt cgccataccg gttgacataa aacagccaga cgagaccatg
     2041 cgggccttgc cagaccaccg ggttgccttc gccacggtcc ggggtgtcgg cgatgactac
     2101 cgggtcggac cacgtggttt cgcctctttt ctggcgtaag ccatatacgg ccgtgtcgtc
     2161 ctcgtattct cccgatccgc cgtagtacgc gagatacagg tcgccgcagt caagctgggt
     2221 gatggatgcg ggatgtttgt actgacccgg gaactccggg ccaatcacgc gatacgtctc
     2281 tatattctcg ctttgggcgg cggcgatgag cggtgccagg ccgagcgcaa gcacggtgac
     2341 ggttttgatc atgatatggg tcctcccagg ctcgtggata tcggtctatt ccgcgctcag
     2401 tgttgcgcgt gaacacgatg cgtgtcaaac gggaaggaag gaatgggctc aggcccacgc
     2461 gaccgcggat atttctttgc tgcccggcgc cgcggacgcc tcgagcgggt ggcctgacgc
     2521 tgtcgaacgc aggccccgcg acacctggtc aatccaatag aggtaccagc gaaggatgac
     2581 gttgaattcg tcgggccgct ccatcatggg ggcatgcccg catttatcga tgaagctcag
     2641 gcgcgacagg gccagtttgt catggaattc gtgggccact tcaggcgggg tgacgacgtc
     2701 gtcgcggccc cagatcagcc ctaccggcat ggtcagatgc cgcagttcgt ccgcaagatt
     2761 ttcgcggacg gcggatttag ccattcgaag cagtttgagg gcctttacgt tactgttgag
     2821 gacttcgaac acctcatcta cgagttcttt ggtggccata tccggatcat agaaggtgag
     2881 agccgctttt tcgcgcacat agtcgtaatc tttgcggcgc ggaagtgtcg tgcccatggt
     2941 gttttctttt aatcccgaac tcccggtgag gatcatgccg gagacatttt cgggatgggt
     3001 gagggcgtac cgcagcgcca catgcccacc gagggaattc cccagcaagg caaccttatg
     3061 gaatctcttg tgcgtgatga attcgtgcag aaactggctc aactcctcga cgcctgtctt
     3121 tcgcagagga aggtcgaaaa gggggagtac gggggccacg gcgcgatagt ctttgcggag
     3181 gtcggtgagc accgactgaa aattctccgg gccgcccatc aagccgtgca ggagaataat
     3241 taccgaaccg gagccttctt caaaataggt ataacctgtt tcagtcttat acgttaacgg
     3301 catgaattct ccgtcgtgaa acacgctggg aagtacgcgt tttggcccct gacccatcta
     3361 ccgctgtatt ctaccgccga gccgggaatt tgttaccgct taatccgcgc cacccctgtc
     3421 cggaggaaga ggcgcctttt ctccccgccg caggatcaaa aggtcttcgc cattgaagag
     3481 ggtaatgttc tgctggaggg gattatactc gcggatgatc catccttccg cgacactgcc
     3541 gcccaattcg cgcgtacgtt cctgcatttt cccatccggg gtgtgaatgc gaataaaaaa
     3601 gcggggggct ttgtcggccg acgtgaccac gcctcgcaac tcaacttcta ccgtatttct
     3661 tttcggggct gaagcgcctc cggcgggttc gccagaggcg tcctcaaacg cgagaacagc
     3721 atcatcgagc gattccggat ccgatggatc ggttcctgcc gcgggggcgc caggcgcggc
     3781 cgtgccgtcc tccgaaagcg gatcgttgcc cagggctgct tccgtttcct gcgctggtgc
     3841 agctattgta atctgcggcg atccggaatg ttgccccgtc tcgttggccg catcagaggt
     3901 gatgtgcgcc ggtcttggtc cgaatatccc cggccggagc cagggcatgg gaatcaagcc
     3961 gtgccacacg ccgaatatta cgcccgccac gatagccgcc atgaacacca ggcccaaaat
     4021 ggcaaacacg gtcaccggcc gggtgcgtgc cggacgcggc tttcccagcc gctcgacgaa
     4081 cgtgccgcgc aatgcgggga ggtcgcggtc ggacggggca gcaatggccc ggtcgatggt
     4141 ttgtaacgct ttctcgaggt cagtccgggc ctgccggagt tcggattcga ggctgtcgaa
     4201 ctggtcgcgg cccagctctc ccagctggtg ccggccacgc gccagctcaa gctgcgagtc
     4261 aatgacgcgc tggtcggatt ggatgtcgtt tcggaaccgc tcaaggcgga attgccagaa
     4321 gcggatcgtt gccgcggccc gggcccgggc ggctttttgc accttggcgg cccgtttgcc
     4381 ttcttttccg gcgtcgccag tctgttcctg gaggcgggac gcggcttcga ggtttatttc
     4441 ggcctgtttg cggtttccga aagctgcccg aagctgatcg accatccagc gaagaagtac
     4501 tgagggttcg gccgcctcct caatcagggg gacccgcatg gagagttcat ccatgctgga
     4561 cagcggcaga tcgatgtagc cacccgccag atcggcatca cgcacgaccc gccacgcccg
     4621 gaggttgatc tgccgccgtt cgaggcattc ccgccgccag cggatgcggc cggcccgctc
     4681 gctgtacatc cggtagtcca gctcgccgac acgcgcctcg agagcaagtt ccgccagctt
     4741 ttcctgcgcc gcggtcagct cgatggaggc ctccatcaaa cgttggtgca attcgtcgtt
     4801 gaattcggcg cgcagcgcct tcaagcgggc tttatactct tccagttcgg atttcgattg
     4861 cccgtccgcg gcctttatct cgcgcgagac cttcttttcg aggctgcata gctttcgcag
     4921 ggtgtcggat gcatcgatag gcaccgaaat gctcgttctc cgccgtttac gagcgtcctg
     4981 ctcctccacc gcgggatctg cggtttgagg cggttcgcgg atgaactccg ggtggtcgcg
     5041 gagaaaaatg tcggcaacgc cccgggggat ttcctcaaaa cgtggaaggc ggatcttaag
     5101 ctggcacgcg atacaccgtg cccggcgtcc atacatggtc tcagtcaggc gcattttctg
     5161 tccacacaga caaaagatgc gtacggcaga cgattcccgg gtgggcatgt cgaagcctcc
     5221 agtgtagctt gcctgtgtta agcgtaccct gctctctaaa tacttgtcaa ttatgggtat
     5281 gagcttatgc cgcccctgta ctcaacgacc cggcggggcg aaagagatgt acgaattgcc
     5341 ggagatctgt caaaacggtg attcagccac ggcgaattga cggatttcag cctcgatacc
     5401 caggcctgtg gcaatcgggc gataagtggt aacttgcatc atgacaaata gttgcgacag
     5461 aatagccgcg gccgggacga agaaaatggg gttggtacgc ggtttgctct cttcaggacg
     5521 gctctcacag acgaggaatt gcacagcctc tgcagccgcc cccccggacc cccagaccgg
     5581 gggggcacct ctttccggcg agtcagatcc ggtcacctgg ggcgcaaccc cgggaaatca
     5641 ttcggcagac aaagtcccac tcgcttgcac gcttctccca gtcagctcct cttagcgttt
     5701 cggtcctgaa attgacaccc gcggcctgga agggtactat ctcgctgtag agcctgtgga
     5761 tttgggggaa gaacagagcg gatttcttaa ggccgccgcc aggagagcgg aatcacgccc
     5821 ctttggaaag aaggctgcga gcggcatccg gtaatcaagc cgtctgaatt cacgtacggg
     5881 ctctcagtgg atgccatatt ccaccagcca atatctgtgc aaggattgac aatgcatgac
     5941 aattcatcct acggccatta ttgatcccaa agctgaactc gccacagatg tagaggtgct
     6001 gccctacagc attatcggtc cgggcgtgac catcggctcc ggcacggtcg tgggccctca
     6061 ttgcgtgatt gacgg
//
LOCUS       DAOPBQ010000186         6065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11430_length_6065_cov_4.390682, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000186 DAOPBQ010000000
VERSION     DAOPBQ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11430_length_6065_cov_4.390682"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1459
                     /locus_tag="PKW60_07360"
     CDS             314..1459
                     /locus_tag="PKW60_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99290.1"
                     /translation="MSGGIFLIKGNKELVRMEEAQHETEAIFQKLLADYPDLLAGEQM
                     NPDSPRKWLLIAREYGVPGEEQGRDLWSLDHLFLDQDGVPTLIEVKRSTDHRIRREVI
                     GQMLDYAANGVAYWPVEKIQARFDALCEEQDVQPEAKLVECFGEEIDQEAFWEQVKTN
                     LTAGKVRLVFVADSIPRELRRIVEFLNEQMDPAEVLAVEIKQYVGQGQKTLVPRVIGL
                     TAEAERKKPSRSGRQWDEERFFKKLTKNCGKDIAETARKILAWAKERFSRIEWGKGRA
                     TGTYTPVLDLAGMSHYLVYVRTNGRILILFDPMKRRPYFDSDENRHELRKRLNAIPGI
                     SIPEDDININPSIDLSVLADPVKLKGVLDALDWALEMIKRECGPQER"
     gene            2031..5153
                     /locus_tag="PKW60_07365"
     CDS             2031..5153
                     /locus_tag="PKW60_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99291.1"
                     /translation="MSLVIAMLVGLAGMGAGEMEASEVEAGEAAAPARILFDGGFVSE
                     GGEAPLRNEGVRLTEGRGGQAAEFQKGAVLTYPVAGGFDPTQGTLSMWVRPNWDSVHS
                     FGERFFWGITNPDGGARIILGFLGGDGQGMVQFGPDGAIEGLAAKVDWRAGEWHHLMV
                     CWDESVPCRALYVDGVLRHRLSGGAGLPRQAEWFHVGSLPCVTRWMGVLEGHEADAAI
                     DEVTLSPTAEAPDFDLVRQAAREDVKAVERNRLSREQAAPAYERAWERFLLSPTLDSV
                     AAEQHEVSWDELGGMAAPMTKRMPIQPRYAAEFVHVQPDLSIALGRARDAFGLGFACG
                     EPFELPDMYAVRRSLHRGYQPIVKSVWNAASCEVHQTALTILPRDEETVTGREPQVAV
                     VRMTVRNTTGEPQRIPLYTLIGRMHGTQNSNYSPFLASASRWLEPSMGLRIDGDAVFA
                     EGRAALVFRAGPQVRVDTLETLETHTANPLFPEVLRNVVAFTFELQSGETATLDLAIA
                     GAPEFFPPEDAALLREITFDAALAWAEAYWDNGLAPGMKLTTPEPRLNNVYRHLILSC
                     LANHRKNPERPWREPFQAPMWEGVWPWESAHMMVPLCALGYHKELEPVLRFFTERQSG
                     IGQYAEPGRKPEGETQSAHGCYTGNFLLRWTSETGSILWALASKFRYSGDTAWLNENK
                     ASMLAAWDWVQGERAHTRQFTETGEKVKYYGMLPRGRVHDWEGWHYFFFSDVYTWKGM
                     VEMAEAFRTAGLPEAERLVAEAAEYRDCLLEAIERAQYVDPDTGLLFVPNLVATREDE
                     QAGLWWADGPSCMFATGLLDARTDERFDAMLAYLEQTWGTMIGLTNRMDRPKELGKRN
                     PFWYVINSERGYFENLLARGEIEKALLILYSNLSYGLSQDCLQTVERIHVSDANYAPF
                     QPNSSGNGRLLDMMRRMVVDDQEPGMLWLLRGCPRRWFEPGESIIVEDAPTYYGKMAV
                     RTHASDDAVVIDVDPPAGKIPVDLRVMVRHPSRSAPKSVTVNGAPGRIEQEAVVLPQA
                     RGHQQIRCQY"
     gene            complement(5304..>6065)
                     /locus_tag="PKW60_07370"
     CDS             complement(5304..>6065)
                     /locus_tag="PKW60_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99292.1"
                     /translation="QAGTESGPKGKRSANRVRELLKKASQEYGLASQVMSLVAVVKRE
                     GDVEGELPKITVIPVGMPQDTDFAGYFAYSAPPASFDTLEWSGTVRLCRMATYSVRDV
                     SSPPDLGIADLRAPASHESHARLASEDRLVELAALLEPDGGMPGDDLEERILASLLAL
                     LAFYGEGHDFNRGPFARHMERLATWLEQADRSMLSDEHRDIITRVLDAIDAKRAVHGF
                     GAEIANKFILEDEMDAPAAWASLRKAFDQSAYGEL"
BASE COUNT         1217 a         1640 c         2032 g         1176 t
ORIGIN      
        1 aggcaaactg cctgtaaatc taaattggac tgttccaaat tgcaacttgc actgggtcat
       61 acattaaccg tttggccaaa ttgcgcagac cgtgtgccct aatgaagatt gggctaaccg
      121 tggtttctcg gaggccggca tgacaacaag gtaccacccc ccgcgccctc cagacggagg
      181 cgttggcagc cttgtctcag acggcggaag atggcatcct gtgacttagt taaacgctgc
      241 caacagccca gtgtacatgc gatcttggcg cgggggatga cgacggcctg caaaggaaaa
      301 ggagtaataa gtaatgtctg gtggaatttt tctcatcaaa ggcaacaagg aactggtacg
      361 gatggaagag gcacagcacg aaacggaggc tatctttcag aagctcctgg ccgactatcc
      421 cgacctgctg gcgggggagc agatgaatcc agactctccg aggaagtggt tgttgatagc
      481 gcgagagtac ggtgtgcccg gggaagagca aggtcgcgac ctctggtctc tcgaccacct
      541 ctttttggac caagacggcg tccccacgct cattgaggtc aagcggagca ccgaccaccg
      601 catccggcgc gaggtgatag gccagatgct ggattatgcc gcaaacggcg tcgcctattg
      661 gcccgtcgag aagattcagg cccgtttcga cgctctttgc gaggaacaag acgtccagcc
      721 tgaggccaaa ctcgtggagt gcttcggcga agagatcgat caggaggcgt tttgggaaca
      781 ggtgaaaacg aatttgactg ccggaaaggt gcggctcgtg tttgtcgcgg acagtattcc
      841 ccgcgaactg cgccgcatcg tggagttttt gaatgaacag atggaccccg cggaagtcct
      901 ggctgtcgag atcaaacagt atgtgggcca ggggcagaaa acccttgtgc ctcgtgtgat
      961 cggtctgacg gcagaggccg agcgcaagaa accctcgcga agcggccggc agtgggatga
     1021 ggagcgtttc ttcaaaaagt taaccaagaa ttgcgggaaa gacatagccg aaactgccag
     1081 gaaaattctc gcgtgggcga aagagaggtt ttcaagaatt gagtggggaa aggggagagc
     1141 gactggcact tatacaccgg tcttggacct ggcggggatg tcacactatc tggtctatgt
     1201 ccgcacaaat gggcgtatcc tcatcctgtt cgaccccatg aaaaggcgcc cctacttcga
     1261 cagcgacgaa aaccgccacg aattgcgtaa acggttgaat gccattcccg gcatttccat
     1321 ccccgaggac gacataaata taaacccttc gatagattta tctgtgctgg cagacccagt
     1381 gaagctaaag ggagtgctcg acgcactgga ctgggcattg gagatgatca agagagagtg
     1441 tggtccccaa gagaggtaag acctgacctc tgtcccgccg gttgagcgct agaaacgcgc
     1501 acgaggcaat gttccagcgc tggagctctt gttgtgccgg tattcgcggc agattgtcgc
     1561 gcaatgccga cccaattcgc gtgcaatcgc tgcgtagctc tatccctgtg cggcaaaaaa
     1621 ttcgatcttc gtgcactcca ttgaggtaag atggatgaag gacatcggga ttccttttgc
     1681 tcttggttga tcgtcacctc caagaatacc gaaggggtcc cgatgtcttt gttcatgaca
     1741 ggtcttcctt taaaactcac ctcctggaaa aaacaaaacc taaaaccgtg cacttagaat
     1801 ttgaatacgg ggacggttgc tgcagtaatg gcttccaaaa tcttttttgg cgcgtccgtt
     1861 gcagatggac atttttgtcg atccacgcca aaattgagcc cggatcgccg ctgcggcgtt
     1921 ctacagtctc gcgggcgtaa gtggaatcca cctcccgctc cccttgcggc ggtggcggtg
     1981 gtgccaggga tataatgcgc tgtggtgtcc ctgtagacag gagcagagcc atgagtctgg
     2041 tcattgcgat gttggtggga ttggcgggga tgggggctgg cgagatggag gcgagcgagg
     2101 tggaagcggg cgaggcggcc gcgccggcgc ggattctgtt tgacggcggc tttgtctcgg
     2161 agggcggtga agcgccgctc cggaatgagg gtgtccgatt gacggagggg cgcggtgggc
     2221 aggcggcgga gtttcaaaaa ggggcggtac tgacgtatcc ggttgcggga ggattcgacc
     2281 cgacccaagg cacgctttcg atgtgggtgc gtccgaactg ggacagcgtg cactcgtttg
     2341 gcgaacgttt tttctggggg attactaatc cggatggcgg ggcgcgcatc atcctgggct
     2401 ttctgggcgg cgacggccag ggcatggtgc agttcggccc ggatggcgcg atcgaggggc
     2461 tcgcggccaa ggtggactgg cgcgcgggtg agtggcatca cctcatggtc tgctgggacg
     2521 aatccgtgcc gtgccgggcg ctgtatgtgg atggagtgct tcgccaccgc ttgtctggcg
     2581 gggccggttt gccgcgccag gcggaatggt tccatgtggg ttcgctcccc tgcgtcacgc
     2641 ggtggatggg cgtgctcgaa gggcatgaag cggatgcggc tatcgatgag gtgacacttt
     2701 cgcccacggc ggaggcgccg gattttgatc tggtgcgtca ggcggcgcgt gaagacgtaa
     2761 aagcggtcga acgaaatcgt ctgtcgcgcg agcaggcggc gccggcctat gaacgtgcgt
     2821 gggagcgttt tctcctctcc cccacgctcg acagcgtggc cgcggagcag cacgaggtgt
     2881 cgtgggacga gttgggcggc atggccgcac cgatgacgaa acggatgccg atccagccgc
     2941 gctacgcggc ggagttcgtg catgtgcagc cagacctttc gatcgccctg gggcgcgcga
     3001 gagacgcgtt cggattgggt tttgcgtgcg gggaaccgtt tgaactgccg gacatgtacg
     3061 cggtcaggcg gagcctgcac cgcggctatc agcccatcgt gaaaagtgtg tggaacgcgg
     3121 cgtcgtgcga ggtacatcag accgcattga cgatcctgcc tcgggatgag gagacggtca
     3181 cggggcgtga gccccaggtg gccgtggtgc ggatgacagt gcgcaataca accggggaac
     3241 cgcagcggat accgctgtat acgctcatcg gccggatgca cggcacgcaa aacagcaatt
     3301 acagcccctt tctggcttcg gcttcccgct ggctggagcc ttcgatgggg ttgcgtatag
     3361 atggggacgc agtgtttgcg gagggccggg cggcgctggt ctttcgggcc gggccgcagg
     3421 tgcgcgtgga tacacttgag accctggaga cgcacacggc gaacccgctg tttccggagg
     3481 tgctgcgcaa cgtagtggca ttcacctttg aactgcagag cggcgagaca gcgacgctgg
     3541 atcttgcgat tgcgggggcg ccggagttct ttccgcccga ggatgccgcg ctgttgcgtg
     3601 aaataacgtt tgacgcggcc ctggcgtggg cggaagctta ctgggacaac gggctggcgc
     3661 cgggcatgaa gctcacaacg ccggaaccgc ggctgaacaa cgtctaccgg cacctgatcc
     3721 tgtcgtgcct ggccaatcat cgcaagaacc ccgagcggcc gtggcgtgaa ccgttccagg
     3781 cgcccatgtg ggaaggggta tggccgtggg aaagcgcaca tatgatggtg ccgctctgtg
     3841 cgttgggata tcacaaggag ctggaacccg ttctgcgctt cttcacggaa cggcagtcgg
     3901 gcatcgggca atatgctgag ccgggccgta aacctgaagg cgagacgcag tccgcgcatg
     3961 gttgttacac cggcaacttt ctgctgcggt ggacgagcga aacgggctca atcctgtggg
     4021 cactggcgtc aaaattccgg tattcgggcg ataccgcgtg gctgaacgag aacaaggcga
     4081 gcatgctggc ggcctgggat tgggtgcagg gtgaacgggc tcacacgcgg cagttcacgg
     4141 agaccggcga aaaggtgaag tattacggga tgctgccgcg gggccgcgtc catgactggg
     4201 aaggatggca ctatttcttc ttctctgacg tctacacctg gaaaggcatg gtggagatgg
     4261 cggaggcctt tcggacggca gggctgccgg aagcggagcg tctcgtagcg gaggccgcgg
     4321 aatatcggga ctgcctgctg gaggcgatag aacgggccca gtatgtggat ccggacacgg
     4381 ggctgctgtt tgtgcccaat ctggtggcga cccgcgagga cgaacaggcc gggctgtggt
     4441 gggcggacgg gccgagctgc atgttcgcca cgggactgct ggacgctcgt acggacgagc
     4501 ggttcgacgc gatgctcgcc tatcttgagc agacgtgggg cacgatgatt ggcctgacca
     4561 accgcatgga cagaccaaag gaactgggca agcgcaaccc gttctggtat gtgatcaact
     4621 ctgagcgtgg atatttcgag aacctgctgg cccgggggga aatcgagaag gccctgctga
     4681 ttctctactc aaacctttcg tatggccttt cgcaggactg cctccagacc gtggagcgaa
     4741 tccatgtgag tgacgcgaat tatgcgccgt ttcagccgaa ctcatccggc aacgggcggc
     4801 tgctcgacat gatgcggcgg atggtcgtcg acgaccagga accgggcatg ttgtggctgc
     4861 ttcgcggttg tccacgccgg tggtttgagc cgggcgagtc gatcatcgtg gaagatgcgc
     4921 cgacgtatta cgggaaaatg gccgtgcgca cgcacgcctc ggacgatgcc gtcgtgatcg
     4981 acgtggatcc gcccgcgggc aagatacccg tggatctgcg ggtgatggtg cgccacccca
     5041 gccggagcgc cccgaaatcg gtgaccgtga acggcgcccc ggggaggatc gagcaggagg
     5101 ccgtcgtgtt gccccaggcg cgcgggcacc agcagattcg ttgccagtat tagcgccggc
     5161 gggcgcccgg cgagccggcg cggaccagtc cgccgggata gtgagcgggc ttcgcctgcc
     5221 ccgatgcgaa aagaaaacgc acggcgggca cggagaccag actccgtgcc cgccgttgta
     5281 aacggattca cgccgcacgc ggattacagc tcaccgtatg cactctggtc gaacgccttc
     5341 cggagggacg cccaggccgc cggggcgtcc atttcatcct ccagaatgaa cttgttcgcg
     5401 atttcggctc caaacccgtg aaccgctcgc ttggcatcga ttgcatcaag cacacgggtg
     5461 atgatatcgc ggtgttcatc gctgagcatg gacctgtcgg cctgctcaag ccatgtggcg
     5521 agccgttcca tgtgccgggc gaaaggcccg cgattgaagt cgtggccttc gccgtaaaac
     5581 gccagcaggg ccagcagaga cgccagtatc cgctcttcca gatcgtcgcc gggcatgccg
     5641 ccgtcgggct caaggagcgc tgccaattcg accagtcgat cctccgaggc aaggcgcgcg
     5701 tgactctcgt gggaggcggg ggcgcgaagg tcggcaatgc cgagatccgg gggcgagcta
     5761 acgtccctga cagaataggt ggccattctg cacaaacgga ctgttccact ccactcgagc
     5821 gtgtcaaagg atgccggcgg agcactgtaa gcaaaataac ccgcaaaatc tgtgtcctga
     5881 ggcatgccga cagggatcac ggtgatcttg gggagttcac cttcgacatc gccttcgcgt
     5941 ttgaccacgg cgacgagcga catgacctga gaggccagtc cgtattcttg actcgccttt
     6001 ttgagcagtt ccctgacacg attggccgaa cgctttcctt tggggccgga ttctgtcccg
     6061 gcctg
//
LOCUS       DAOPBQ010000187         5998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11566_length_5998_cov_4.146222, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000187 DAOPBQ010000000
VERSION     DAOPBQ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11566_length_5998_cov_4.146222"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PKW60_07375"
     CDS             <1..717
                     /locus_tag="PKW60_07375"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPJ99293.1"
                     /translation="LPAEEPEIEDTFLPTAGIVKFVWSAGGQRAVLAAKGGHNGEPHN
                     HNDVGSFVLRFGDTTCLCDPGAGLYSRDYFSPKRYDNVFANSYGHSVPRLGGALQSTG
                     EQFRGTVDVPAPKQAHIDLTGAYQVEGLTKAERVFSVQEDGMLAMDTRYTFDGPGQDV
                     EEVFMTWLEVEIDGPTARVRSKDGVLEIRAAAGAFATESLEDACKANHKSGVLTRLTL
                     LKPAAAEIQNHYTFTFFPAE"
     gene            787..1182
                     /locus_tag="PKW60_07380"
     CDS             787..1182
                     /locus_tag="PKW60_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99294.1"
                     /translation="MLIRLGTATASWLGHLLGKAGQWLGAPPPDRYGHITEYRSNPLV
                     ARMRLTGIVFGNVVLYSVGEPAIRVPRYDRCGYVELREHEWAHVAQYQGWGALFWIKY
                     GLAECVRPFTVSRINRYEMLADDSCRKPS"
     gene            1372..1503
                     /locus_tag="PKW60_07385"
     CDS             1372..1503
                     /locus_tag="PKW60_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99295.1"
                     /translation="MAVLELLDVSKRKFPGVLRYEAALKPVRATDASAGYELGETCM"
     gene            1754..5686
                     /locus_tag="PKW60_07390"
     CDS             1754..5686
                     /locus_tag="PKW60_07390"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HPJ99296.1"
                     /translation="MGMRFFSLTATVVAGVVLAIVLAGCPKAPEASLALLVTPKSLDF
                     GATETALPLKVSKNYTSVPLGQFRASSSVSWLSVSPSSGTSSGPDDPATILVTVHREL
                     MNAGESLGSIILTAQGASQVSVPVKAFRRIGAAFSVSSNDAFVGDELRFIDQSRAVGG
                     APPISSWMWDFGDGTTSNSPNPTHIYSAEGSYTITLTVSNGEETAALTRQHFVTVRAR
                     VAPVADFQADAVEVYPGANVQFSSTSNEGTGAIISYAWNFGDGEAGSGRDPVHSYTNV
                     GTYSVSLTVTTAHGQDTRIRQNYITVLPYAPEAAFSSDVQRAVLGVNGGRVAFSDESE
                     PGSFPITDWFWEFGDGETSGEPEPVHTYHQTGFYTVSLTVTAENGQMNTFTALNYIEV
                     IPNDIAAEFALENHNAEVEQPVQFTDLSAPGPSPITQWRWDFGDGESSYERHPLHVYH
                     NAGTFTVSLTVTNAYGFDEEVKVDCITVWPISTLKRYINDSGPASYTVYDIVPVPYGG
                     KLLTAHILDLHSQTFRPGEVEPAEWHHWMVVITPSTLVTDTALMIISGGSNRATPNFD
                     PNNVTGERAVALEFVARTGAAAILLPTVPNQPLVFHGDGIGRKEDEAIAYTFDQFLNG
                     GDEYWPLLLPMTRSAVAAMDVVQNYGAIRGFNIQDFVITGASKRGWTTWLTGAADDRV
                     RAIAPLVIDVPNMRTQMNYHRQAYNGYSYAVQDYVDFDVFDRFGTERGQELLKIVDPY
                     SYLWALDMPKLIVNSTGDQFFLPDSAQFYVHELPGETRLRYMPNTDHGIEQGSSLTDL
                     GESLIGFFTYVANDYPLPSFTWEVTRDNEIRVHTQRASVPISVRLWSATVSGTMRDFR
                     FASTGAIWSSQTLRDPDGDGTYLATVPTPTAANTWTGFFVALTFRDRGQAQTFCTELR
                     VTPDVMPFGPPVLRVDKDAAGAGDGSSWSNAFTSIQEAIVAATGAGFHEIWVAEGVYE
                     EAIMLESDLALYGRFKGTEHALFDRDINAHPVIIDGRAAAGGLPAKHVVTIEQKSNIR
                     LDGFSITGGDASTVIPGDWSATAGGGIYCSQLDETVVITNCKIYGNRAANSGGGIACS
                     GFRDGAHLVECSPVIENCIVTDNYGALGAGGILCFAGANPTIVNTIVTDNRCTGIANE
                     WGGGGIGCVDASSPAIANCILARNTTTAQTGGLSAGSLIWGGDCDPVCVNTIFFENGD
                     IAVYEGTTDSDVTLKHCLFFGNTGGDVWDENSTLYTGAAQINANIAEANLCVSGDPEF
                     VDADNDDFRVRPGSPAVDAGTADGAPDTAIDGTPRPQGGAIDIGPYEQ"
     gene            5812..>5998
                     /locus_tag="PKW60_07395"
     CDS             5812..>5998
                     /locus_tag="PKW60_07395"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPJ99297.1"
                     /translation="MRAVIQRVSEASVSVDGRVTGRIGPGLLVLLGVGQDDSEEDARL
                     MADKIAGLRCFADAESKF"
BASE COUNT         1203 a         1788 c         1798 g         1209 t
ORIGIN      
        1 ctcccggccg aagagccgga gatcgaggat accttcctgc cgaccgcggg catcgtgaag
       61 ttcgtatggt ccgcgggcgg ccagcgtgcg gtgctggctg ccaaaggagg gcacaacggc
      121 gaaccgcaca accataacga cgtgggcagc ttcgtgctgc gcttcggcga taccacctgc
      181 ctgtgcgatc ccggcgctgg actgtacagc cgcgattatt tcagccccaa acgttacgac
      241 aacgtgttcg cgaactccta tggccacagc gtcccgcggc tcggcggcgc gttgcagtcc
      301 acgggcgagc aatttcgcgg cactgtagat gttccggcgc cgaagcaggc gcatatcgac
      361 ttgaccggcg catatcaggt cgaaggtctc acgaaggcgg agcgcgtttt ttcagttcag
      421 gaagacggca tgctcgccat ggacacccgg tatacgtttg acggcccggg gcaggatgtc
      481 gaagaagtct ttatgacctg gctcgaggtc gaaatcgatg gcccgactgc ccgcgttcgt
      541 tcgaaagacg gcgtgcttga aatccgtgcc gccgccggcg cgtttgcgac ggaatccctc
      601 gaagatgcct gcaaagccaa tcacaaaagt ggcgtgttga cacgacttac gctccttaaa
      661 cccgccgcgg cagaaatcca gaaccactac acgtttacct ttttccctgc cgaataagcc
      721 gggcaggccg gtgtgaaagg ccggggttgc gtctgacacg gccgcggcat tacggagaaa
      781 ccgcttatgc tcatccggct gggtacggcg accgcgtcct ggctggggca cttgctcggc
      841 aaggccggtc aatggctggg cgcgccaccg ccggaccgtt acggacacat caccgagtac
      901 cggagcaacc cccttgtggc gcggatgcgc ctcaccggca tcgtgtttgg caatgtagtg
      961 ctctattccg tgggagaacc ggccatccgg gtgccccgtt acgaccggtg cgggtatgtc
     1021 gagcttcggg agcacgagtg ggcgcatgtc gcgcagtacc agggctgggg ggcgctgttc
     1081 tggatcaaat acggcctggc ggaatgcgtc cgcccgttta cggtaagccg catcaaccgc
     1141 tatgagatgc tggctgacga ttcctgccgc aaaccgtcgt aagctggcag tggtccggcc
     1201 aaccggacaa caggatgtaa tttattgaat gtcaaagaga tacatccata aaaggaagcc
     1261 tctgacggct ccacaccgtc cgcgtggaac aaaattgaaa cacatgcgtt tcagtgaggt
     1321 ttttgagcct tttcgtaata tatcgttgaa agtccgagag ttagcgctga tttggcagtt
     1381 cttgagctgc tcgatgtttc caagcgcaaa ttccccggcg tgctccgtta cgaggcggct
     1441 ttgaaacccg tgcgcgccac cgatgccagt gcaggatacg aactgggcga gacctgcatg
     1501 taatctcctg atctccgtca actctcgcaa atctcctcta caccataatt cgaaaatcac
     1561 ggcaacgtga catttcagac gagttaaagc ctgcggcatt ttgtaatagt cctggcaacg
     1621 gccagtaaaa cgtgttgacc ttttcgcccc tccttggcat catgaagcat cgtggcaact
     1681 gttgtaagtg tattggtgtc atcctaaaag gctgcacaca ataaacagac tctcaattct
     1741 gggggggtaa tccatgggca tgcgcttctt ttccttgacg gcaaccgttg tcgctggcgt
     1801 agtgctcgcc attgtcctgg cgggttgtcc gaaagctccg gaagcttcgc tggcactcct
     1861 cgtcacgccg aagtcgcttg atttcggcgc aaccgaaacg gccctgcccc tgaaagtaag
     1921 caagaattac acgagtgtcc cgctcggtca gttccgggca tccagcagcg tctcctggct
     1981 gtcggtatcc ccgtcgtccg ggacgagttc agggcccgat gatcccgcta ccatcctggt
     2041 aacggtacac cgcgaactta tgaatgcggg ggagagcctt ggaagcatca ttcttacggc
     2101 tcagggggcc tcgcaagtct ccgtgccggt caaagccttc cgccggatag gcgccgcgtt
     2161 ctctgtcagc agcaatgatg cgttcgtggg cgacgagctg cgatttatag atcagtcgcg
     2221 cgccgtgggc ggcgctccgc cgattagcag ctggatgtgg gactttggcg acggcactac
     2281 gagcaattcc ccgaatccga cgcacatcta ctctgcggaa ggctcgtata ccatcaccct
     2341 gacggtctcc aacggcgagg agacggccgc gctgacccgg cagcacttcg tgactgtccg
     2401 cgcgcgcgta gcgccggttg ccgattttca ggcggatgct gtagaggtct atccgggcgc
     2461 caacgtccag ttcagcagca cctccaacga aggcacgggc gccattatca gctacgcctg
     2521 gaatttcggc gacggtgagg ccggctcggg cagggatccg gtccacagct acaccaatgt
     2581 ggggacgtac tcggtctctc tgacggtgac gactgctcac gggcaggaca cgcggatccg
     2641 ccagaactac atcacggtgt tgccctacgc acccgaggca gcgttcagct cggacgttca
     2701 gcgggctgtg ctgggcgtca atggcggacg ggtggcgttt tccgatgagt ctgagcccgg
     2761 ttcgtttccc attaccgatt ggttctggga gtttggagac ggcgaaacca gtggggagcc
     2821 cgagccagta catacctacc atcagaccgg cttctatacg gtttctctta ccgtcacggc
     2881 ggaaaatggc cagatgaaca ccttcacggc gctgaactac atcgaagtca tcccgaacga
     2941 catcgccgct gaattcgctt tggaaaacca caatgccgag gtcgagcagc ccgtgcagtt
     3001 tacagacctg tcggcgcccg gcccatcccc gatcacgcaa tggcggtggg atttcggcga
     3061 cggcgaatcc agttacgaac ggcatccgct tcacgtgtac cacaacgcgg gcaccttcac
     3121 ggtgtcgctt acggttacga atgcctatgg tttcgacgaa gaggtgaagg tggactgtat
     3181 caccgtatgg cccatttcca cactcaaacg ttacattaac gattccggac ccgcgtccta
     3241 caccgtgtac gacatcgtgc cggttccgta tggcgggaaa ctcctgaccg cgcatattct
     3301 ggacctccat tcacagacat tcaggcccgg ggaagttgag ccggccgagt ggcatcactg
     3361 gatggtggtg atcacgccgt cgacgcttgt gaccgacacc gcgctgatga tcatctccgg
     3421 cggcagcaac cgggctacgc cgaacttcga tcccaacaac gtgacgggcg aaagggcggt
     3481 tgcactggaa tttgttgcac gcaccggtgc tgcggcgatt ctgctgccca ccgtgccgaa
     3541 ccagccgctg gtattccatg gagacggcat cggccggaag gaagacgagg ccatcgccta
     3601 tactttcgat caattcctca acggcggcga cgaatactgg cctctgcttc tccccatgac
     3661 ccgaagtgca gtcgccgcga tggatgtggt gcagaattat ggcgcgatcc gcggattcaa
     3721 catccaggac ttcgtgatca ccggcgcatc gaaacgcggc tggaccactt ggctgacagg
     3781 agccgcggac gatcgcgtgc gggccattgc gcccctcgtg atcgatgtgc cgaacatgcg
     3841 gactcagatg aactatcacc gccaggccta caacggctat tcctatgcag tgcaggatta
     3901 cgttgatttc gacgtgtttg accgtttcgg cacggaacgg gggcaggaat tactcaagat
     3961 cgtggatccc tattcctatc tctgggcgct ggatatgccg aagctgatcg tgaactcgac
     4021 cggagaccag ttcttcctgc cggattccgc gcagttttat gtgcatgagc tgccgggcga
     4081 aacccggctc cgctacatgc caaacaccga ccacggcatc gaacagggct cgtcgcttac
     4141 ggacctggga gaaagcctca tcggtttctt cacgtatgtt gcgaacgatt accccttacc
     4201 gtcattcacc tgggaagtga cccgcgacaa cgagatccgt gtgcataccc agcgcgcgtc
     4261 tgtccccatc agcgtgcggc tctggagcgc caccgtcagc gggaccatgc gtgacttccg
     4321 cttcgccagt accggcgcca tctggtcatc gcagactctg cgtgatcctg acggtgacgg
     4381 cacctatctc gccactgtgc ccaccccgac cgcggcgaat acgtggaccg gcttttttgt
     4441 cgcgctcaca ttccgggacc gcggtcaggc gcagacgttc tgtaccgaac ttcgcgtgac
     4501 gcccgatgtg atgccgttcg ggccgcccgt gttgcgagtc gacaaagacg ctgcaggcgc
     4561 gggtgacggc agttcgtgga gcaatgcctt cacaagcatt caagaggcca tcgtggcggc
     4621 gaccggtgcg ggattccacg agatctgggt tgccgagggc gtttatgaag aagcgatcat
     4681 gctcgaaagc gacctcgccc tgtatggcag attcaaaggg actgagcatg ccttgttcga
     4741 tcgggatatc aatgcccatc ccgtgatcat cgacggccgc gccgcggcag gcggcctgcc
     4801 cgcgaagcat gtggtgacga tcgaacagaa gagcaatatc cgcctggatg gattttccat
     4861 cacgggcggc gacgcgtcca ccgtgatccc gggcgactgg tcggccaccg cgggtggcgg
     4921 tatctattgt tcccagctcg acgaaaccgt tgtgatcacc aactgcaaaa tatatggcaa
     4981 ccgggccgcc aacagcggcg gcggcatcgc ctgctctgga tttcgtgacg gcgcgcattt
     5041 ggtggaatgc agccccgtca tcgaaaactg catcgttacc gacaattatg gtgcgctcgg
     5101 ggccggcggt attttgtgct tcgccggcgc caatccgacc atcgtcaaca cgatcgtgac
     5161 ggacaaccgt tgcacgggca tcgccaacga atggggcgga ggcggcatcg gctgcgtgga
     5221 tgcctcgtcg ccggcgatcg ccaactgcat ccttgcgcgt aacacaacga ccgcgcagac
     5281 gggcggcctg agcgcgggtt cgctgatctg gggcggagac tgcgacccgg tgtgcgtcaa
     5341 caccattttc ttcgaaaatg gcgatatcgc cgtatacgaa ggcacaaccg actcggacgt
     5401 cacgctgaaa cactgtctgt tcttcggcaa taccggtggc gatgtgtggg atgaaaacag
     5461 cacgctctac accggcgcgg cacagataaa cgcgaacatt gctgaagcca atctctgtgt
     5521 gtccggcgat cccgaattcg tcgacgcgga caacgatgac ttccgtgtca gacccggctc
     5581 gcctgccgtc gatgccggga ccgctgatgg cgcgcctgat acggcaattg acggtactcc
     5641 acggccacag ggcggtgcca tcgacatcgg gccctacgag caatagagcg gataacatac
     5701 gtgacgctgc cagggctttt gacgccctgg cagcgccttg atagactttc cgcgcgccaa
     5761 gttatctagt cggcctgtgg tttttcaggg gtagcggaaa ggaaagcatc attgcgggcg
     5821 gtcattcagc gtgtatccga ggcgtccgtt tcggtggatg gtcgcgtgac cggccgcatc
     5881 ggcccgggcc tgcttgtatt gctcggcgtg ggccaggatg acagcgagga agacgcgcgg
     5941 ctcatggcgg acaagatcgc cgggctgcgc tgttttgcgg atgccgaaag caagttca
//
LOCUS       DAOPBQ010000188         5997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11568_length_5997_cov_3.735611, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000188 DAOPBQ010000000
VERSION     DAOPBQ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11568_length_5997_cov_3.735611"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="PKW60_07400"
     CDS             <1..1079
                     /locus_tag="PKW60_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPJ99298.1"
                     /translation="AEYLAGRFREFSLDVELDDFHSVDSEWLLYALYFSEFAVVAIVA
                     AWWPLAAFGYGLLVFLCYLAESTGYRLFGRFLPQYDSQNVVCRFVAPKPRRLCIVTAH
                     YDSPRDSPLQAPGVRPWLRPIQLFLVLCMVLVVVTCAVQGFGLFDSLTFPYDIAVRWT
                     AVGCLLGAAAFLMFTELSCDYGRGAWNNASGTAVLLELVRRFSVDGLEQTDLWFVATG
                     SKEAGLNGMRHFMSTHQFDPASTFFLNIDSVGAPRLHYVIAEGMLSTLHSSKVIVSAA
                     EQAAHAFQATPIRCREFQSDALIPLARGFHGLTLTSSYETPPEDRLLSDDTHLDVRDN
                     ALLAASGLAEAILRRLDHPPGAGQ"
     gene            complement(1241..1735)
                     /locus_tag="PKW60_07405"
     CDS             complement(1241..1735)
                     /locus_tag="PKW60_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPJ99299.1"
                     /translation="MVSKKMEKALNKQINAEMYSAYLYAAMGAYFDAQNLDGFATWMR
                     AQAQEEMEHAMKFYAYIVETGGRVIFEAIDKPPAEFGKPEKVFEEVLKHERKVTRLIN
                     ALVELARSEKDYATDNFLQWFVAEQVEEEATADGVLQKLKMIAGHPNGLFMMNAKLGE
                     RKEP"
     gene            complement(1983..3311)
                     /locus_tag="PKW60_07410"
     CDS             complement(1983..3311)
                     /locus_tag="PKW60_07410"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPJ99300.1"
                     /translation="MHRRAFIQSLTLTPMLAGARAESELRAVEPPPLVANSYVKVYES
                     PDATQIYSYSPGLAVLPSGRLVATLDLGGPAFAGKEGPDGYRGRIYTSDDRGQTWTLR
                     ASTPMMHARPFAAGEAVYVLGHHNDLGIVRSNDDGTTWSEASWLTQGQRWHQAPCNVH
                     YTNGRIYLVMERDTDPDYPMWPVSVFAPVLMAANVNDDLTSRAAWIFSNELSYREIIA
                     AVGPPHLLGIPFHTPGNTVADTARVRRPMADPGWLETNVVQFVDPNHVWFDPEGRTFH
                     LWMRAHTGSTNLAAIAKAVESGDGKITVSLENAPSGAPMMYVPCPGGHMKFHILYDEP
                     TKLFWLLSSQTTDSMTRPDRLPDDRFDLPNNERHRLTLHFSKNCVDWCFAARVAQTNA
                     YGQARHYASMVIQDDDLHVLSRSGDARARSAHDGNIITFHTVRDFRNLVY"
     gene            complement(3337..4221)
                     /locus_tag="PKW60_07415"
     CDS             complement(3337..4221)
                     /locus_tag="PKW60_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPJ99301.1"
                     /translation="MLLQIDSECVGRITKPHYVEVGWRESMNFAASIGDANPHYFDDT
                     RADGIIAPPMLATALTWPVFVNRHEYWGMDAWPEAVCARQVHFSEAILMHRPLRPGGR
                     LAIRGHISGVLPQRGGTLTVVRFDAEDSEGQPVFTEYAGAFFRGVRCADAGRIDGTLY
                     TPPPAPENQDNGWEEVIHIDPLAAHVYDGCTDLSFPIHTSEKFARGVGLPGPILQGTA
                     TLAISLRELVNREAGGDPTRVKELSCAFRGMVRPGSDIRLRLLARIDSEEGVRLFYET
                     LNGEEKKAISDGYVLLST"
     gene            complement(4377..5558)
                     /locus_tag="PKW60_07420"
     CDS             complement(4377..5558)
                     /locus_tag="PKW60_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPJ99302.1"
                     /translation="MRARLKQAIQGITGLAIGVLILWWMFQGTSWSELKRSFAEIRWG
                     WLILAILLIGVSFLGRVKRWSYVVRAVEPGASFRTMFSATQIGFLANFTLPARIGELV
                     RAVVLSRLTPLGVTRSFALVALDRVNDLIGLIVTMAIALLAFRPEQTIEIPGELFRSD
                     SPILVPASAYHTGALGASVFLVIVIAVFVLLFVSKDRVLRISDGLFEFASQRLIPFRA
                     GATACRKFGDWVHRLLRQFAEGFEVFRSSSDLAKSVGYNLATWACFILILQCVLFGFG
                     IQAPWYTVFVMQAALAIAISVPGTPGFVGQFQVPLVAGLKLVQPDVSYNDALALAIVA
                     HAINAGTVIITGLVCLQLEGLHLLQLSDEGALKKQTLGAPGELPENSDGCDKPVTRES
                     L"
     gene            complement(5628..>5997)
                     /locus_tag="PKW60_07425"
     CDS             complement(5628..>5997)
                     /locus_tag="PKW60_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99303.1"
                     /translation="SMETLAEASGLTHDEVRQLLRKTPALRRDLARREPRVCTRCRNQ
                     ALEEESGLCLSCRMALLERLRVAYAELEHKADKRRRQRRGWLPPDEERRGGVVETLER
                     KRGKAPLKKLDPSPRGRFRS"
BASE COUNT         1163 a         1882 c         1725 g         1227 t
ORIGIN      
        1 ccgccgagta tctggccgga cgcttcagag agttttctct ggacgttgaa ctcgacgatt
       61 ttcacagcgt tgacagcgaa tggctgctct acgccttata tttttccgaa tttgccgtag
      121 tggccatcgt tgcggcctgg tggcccctgg cggcgttcgg atacggcctg cttgttttcc
      181 tgtgctacct tgccgaatct accggatacc gtcttttcgg ccgcttttta ccccagtacg
      241 actcgcaaaa cgtggtctgc cgcttcgtgg ccccaaaacc ccggcgcctc tgtattgtca
      301 ctgcccatta cgactcgccc cgcgacagcc ccctgcaggc gccgggtgtg cggccgtggc
      361 tgcgccccat acagctcttt ctcgtgttat gcatggtgct ggttgttgtg acctgcgcgg
      421 tacagggctt cggactcttc gattcactca cgtttcccta cgacatcgcc gttcgctgga
      481 cggccgtcgg ctgcctgctc ggcgcggccg cctttctgat gtttactgag ctatcctgtg
      541 attacggccg gggcgcatgg aacaatgcgt ccggaaccgc cgtcttactt gagctcgtgc
      601 ggcggttttc cgtggatggg ctggaacaga ccgacctctg gttcgttgcc acgggaagca
      661 aggaggccgg cctcaacggc atgcgccatt tcatgagcac gcaccaattc gatccggcct
      721 cgaccttctt tctcaatatc gactctgtcg gagcgccccg ccttcattac gtcattgcgg
      781 aaggcatgct gtcgacgttg cacagctcaa aagtcattgt ctccgcggcg gaacaggccg
      841 cgcacgcatt tcaggccact cccatccggt gccgggaatt tcagtcggat gcccttatac
      901 cgctcgctcg cggcttccat gggctcacgc tcacgtcgtc atacgagact ccaccggaag
      961 accggctgct gtccgatgac acacacctgg atgtccgcga caacgcgctg ctcgccgcct
     1021 ccggactggc cgaggccatc cttcgccgcc tggatcaccc tcccggcgcc ggccagtgag
     1081 gctcgccttg cccatttccc cggtcaatta gctgcacgca gccggctgga ggatgctgcg
     1141 gtgaaaaaaa gacccggcgg gctcttgtga acctgccggg ggtgtggttg agtgctcttg
     1201 gggaacgacc agcaggcgct gccggtcatt ccagacgggt tcagggttcc ttgcgttcgc
     1261 cgagcttggc gttcatcatg aaaaggccat tggggtggcc ggcgatcatc ttaagtttct
     1321 gcaggacacc atcggccgtc gcttcttctt ccacctgttc cgcaacaaac cactgcagga
     1381 aattgtccgt ggcatagtct ttttcgctgc gagccagttc taccagcgca ttgatcaggc
     1441 gcgtgacctt gcgctcgtgc ttcagcactt cttcaaaaac cttctctggc ttgccaaact
     1501 ccgccggcgg cttgtcgatg gcctcaaaga tgacccgccc gcctgtctcc acgatgtacg
     1561 cgtagaattt catggcgtgt tccatctctt cctgcgcctg agcgcgcatc caggtggcga
     1621 acccgtcgag gttctgggcg tcaaagtacg cccccatcgc agcataaaga taggccgaat
     1681 acatctcggc gtttatctgt ttgttcaaag ctttttccat ctttttgctg accatggtac
     1741 ttcctttccc gcttggcggg gatgctacgt catcacatcc cggccgtttc tgttgtggag
     1801 attcgctccc cctgaagtcc cgccgcggaa ccccgggttc gtctgcgacg tagaacaggt
     1861 acttcctccg cccagccggt acacacgcgt accgcccggg ctttgtgctc gttgatgctc
     1921 agtgaaacac gtgagttaag gcattcattc cgcagcgctt gcccaaacgc cggctcaacc
     1981 ggctaataca ccagatttcg aaaatcacgc acggtatgga aggtgatgat attcccgtcg
     2041 tgcgcgctcc tggcccgggc atcgcccgat cgcgacagca cgtgcagatc atcatcctga
     2101 atcaccatgg aggcgtaatg ccgggcctgc ccgtaggcgt ttgtctgcgc tacacgcgct
     2161 gcaaagcacc agtccacgca gtttttcgaa aaatgaaggg tcaggcggtg ccgctcattg
     2221 ttcggcaaat cgaagcggtc gtcggggagc cggtccgggc gcgtcatcga atccgtggtc
     2281 tgagaagaca agagccagaa cagcttcgtt ggctcgtcgt atagaatgtg aaatttcatg
     2341 tgtccgcccg ggcacgggac gtacatcatg ggcgcccctg acggcgcatt ctcgagagaa
     2401 actgtgatct tgccatcgcc agattccacc gccttggcaa tggccgccag gttcgtcgac
     2461 cccgtatggg cgcgcatcca gaggtgaaag gtccggccct cagggtcgaa ccagacgtgg
     2521 ttcgggtcca caaactgaac tacattggtt tccagccatc ccggatcggc catcggacgc
     2581 cgcacccggg cggtatccgc caccgtgttg cccggcgtgt gaaatggtat gccgagcagg
     2641 tggggaggcc caaccgccgc gataatttcg cggtacgaca actcgttcga gaagatccac
     2701 gcggcgcggc tggtcagatc gtcgttcacg tttgccgcca tgagcaccgg cgcaaatact
     2761 gaaaccggcc acatcggata gtccggatcg gtgtcgcgct ccatcaccag atagatacgg
     2821 ccattcgtgt aatgcacatt gcagggtgct tgatgccagc gctggccctg ggtgagccag
     2881 gaagcttcac tccacgttgt gccgtcatca ttcgaacgga cgataccgag gtcgttgtga
     2941 tgtcccagca cgtatacagc ttcgcccgcg gcaaaggggc gggcatgcat catgggcgtg
     3001 ctggcccgga gcgtccaggt ctgcccgcga tcgtcgctgg tgtagatgcg cccacggtaa
     3061 ccatccgggc cttccttgcc cgcgaaggcc ggtccgccca ggtcaagtgt tgctacgagg
     3121 cgtccgctgg gcaaaacagc cagaccgggg ctgtatgaat agatctgtgt ggcgtcaggg
     3181 gattcatata cctttacata tgaattggcc accagcggcg gcggctcgac tgcccgcagc
     3241 tcactctcgg cgcgcgcgcc ggccaacatg ggggtcaaag tcaacgattg aataaatgct
     3301 ctgcgatgca tggtcacctt ctccggagta tatgtattac gttgacagca agacgtatcc
     3361 gtccgagata gccttttttt cctcgccgtt taatgtttca taaaacaggc ggacgccttc
     3421 ctctgaatca atgcgcgcga gcaggcgaag ccggatgtcc gagcctggcc ggaccatgcc
     3481 ccggaaggcg cagctcagct ctttcacccg cgttgggtcg ccgcccgctt cgcgattgac
     3541 cagctcacgc aatgaaatgg cgagtgtggc cgtgccttga agaattgggc ccggcaagcc
     3601 tacgccacgg gcaaactttt ccgacgtgtg gataggaaac gacaagtccg tgcagccgtc
     3661 atacacgtgc gcggccagcg gatcgatgtg aatgacctct tcccagccgt tgtcctggtt
     3721 ttccggagca ggtggagggg tatagagcgt gccgtcgatt cgtcccgcat ccgcgcaccg
     3781 cacaccgcga aagaaggcgc ccgcgtactc cgtaaagacc ggctggcctt cactgtcttc
     3841 cgcgtcaaac cggacgaccg tcagggtgcc tccgcgttgc ggcagaactc cggaaatgtg
     3901 cccccggatg gccaggcggc ctcccggccg caaaggccgg tgcatgagga tggcttcgga
     3961 aaaatgtacc tgccgcgcac agacggcctc cggccacgca tccatgcccc agtattcatg
     4021 gcggttgacg aagacaggcc aggtcagcgc cgtggccagc atgggcgggg caatgatgcc
     4081 atccgcgcgg gtatcgtcaa aataatgcgg gttggcatcg ccgatcgacg ccgcgaaatt
     4141 catcgattcg cgccacccca cttcaacata gtgcggcttg gttatgcgtc caacgcattc
     4201 ggaatcaatc tgcaagagca aggtcattct cctccgccgg aaccttaaca cgttaagctc
     4261 agataatgca cttttccgcc acaaccggcc ggccgcatgc gggcggccct gcgacaggcc
     4321 gtacgttgaa aaaacgcctg aaatacgccc cgaaagccgt gccagccgcc tggttcttaa
     4381 agggattcgc gggttacggg cttgtcacat ccgtcgctgt tttcgggcag ttccccgggg
     4441 gcgccgagcg tttgtttttt cagcgcgcct tcgtcagaca gctgcaatag atgcaacccc
     4501 tcgagctgga gacacacgag accggtaatg atgaccgtgc ctgcgttgat ggcgtgggcc
     4561 acaatggcca gggccagggc gtcgttgtat gagacatcgg gctgcaccag tttcaaaccc
     4621 gccaccaacg gcacctgaaa ctggcctacg aagccgggag tgcctgggac gctgatcgcg
     4681 atggccaacg cggcctgcat cacgaatacc gtgtaccacg gcgcctgaat cccaaacccg
     4741 aacagcacgc attgaagtat gaggatgaag cacgcccagg tggccaggtt gtaccccacc
     4801 gatttcgcca gatccgaaga agatcggaac acctcgaacc cttcggcgaa ttgccggagc
     4861 aggcggtgaa cccagtcccc gaacttgcgg cacgccgtgg ctccggcccg gaacgggatg
     4921 agccgctggc ttgcgaattc aaacagcccg tcggaaatac gcaggacccg gtctttactg
     4981 acgaacagca acacgaagac cgcgatgacg atgacaagaa acacggatgc gcccagcgcg
     5041 ccggtatgat acgcgctggc tggcacgaga atgggggagt cgctccggaa caattccccc
     5101 gggatctcga tcgtctgttc cgggcggaag gccaggagcg ctatcgccat ggtcacaatc
     5161 aatccgatca ggtcattgac ccgatcgagc gctaccagtg caaagctgcg cgtaaccccg
     5221 agcggagtca gacgggacag caccaccgcc cgcaccagct ccccgatacg ggccgggagt
     5281 gtgaaatttg ccagaaatcc gatttgcgtg gccgaaaaca tggtccggaa ggaggccccc
     5341 ggttctactg cccgcacaac atagctccat cgcttgacac gtcccaggaa actcaccccg
     5401 atcaacagta tcgccagaat aagccagccc cagcgaattt ccgcgaacga acgcttcagt
     5461 tccgaccaac tcgttccctg aaacatccac cagaggatga gcacaccaat cgccagccct
     5521 gttatgccct gtatggcctg cttgagtctt gcccgcaaca gaagttctcc agtgagtagg
     5581 gtgttatgtt gtgcacagtg cccgcggcat caagtcagga aatggagtca ggagcggaaa
     5641 cggcctcggg gcgacggatc aagctttttc agcggagctt tgccgcgctt ccgctcgagc
     5701 gtttccacca cgccaccacg ccgttcttcg tctggaggca gccagccccg ccgctgcctg
     5761 cgccgcttgt ccgctttatg ctccagttca gcatacgcca cgcgcaatcg ctccaaaagg
     5821 gccatccggc aggaaaggca cagccctgat tcttcctcga gcgcctgatt gcggcagcgt
     5881 gtgcagacgc ggggttctct gcgcgccaga tcacggcgga gggctggagt tttgcgcagc
     5941 agttggcgca cttcatcgtg cgtcaagccc gaggcctctg cgagcgtttc catgctg
//
LOCUS       DAOPBQ010000189         5984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11600_length_5984_cov_3.932704, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000189 DAOPBQ010000000
VERSION     DAOPBQ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11600_length_5984_cov_3.932704"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKW60_07430"
     CDS             complement(<1..662)
                     /locus_tag="PKW60_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPJ99304.1"
                     /translation="MSDHAEPGAVPGIIFGTNAVGEALRAQGRVNRLYLAKDAKVREK
                     QALVDLARTQGIPYDFVPLARLNELAGSRDHQGVLARISPVAYTELDAFLASCPPQAT
                     VLALDGVQHPKNLGMLIRTAVGAGCHALILAARGGALLDESVVRASAGALFHLPVIMT
                     TKFPDTLRQLQEAGFWVYGMDPAGRESVFTVSWAERCVVVMGNETKGMRGPARKCCDA
                     LVR"
     gene            complement(655..981)
                     /locus_tag="PKW60_07435"
     CDS             complement(655..981)
                     /locus_tag="PKW60_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPJ99305.1"
                     /translation="MPFRDDELEISFYKSRGPGGQKKNVTESAVRVRHLPTGLVVTAS
                     ESRSQHRNKAAALEELERRLALRRRRRKPRVATRPSAASRRRRADSKRRLSEKKELRR
                     RPETDE"
     gene            complement(1201..4332)
                     /locus_tag="PKW60_07440"
     CDS             complement(1201..4332)
                     /locus_tag="PKW60_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HPJ99306.1"
                     /translation="MSSDPLLAPGAYARILDEELKDLIAHSPGLQPTLRKLEEEEEPE
                     QFNLLLGQVFLEALRQCKSATRANLLNSLIELLGAQEGLDYLVRKRILHPLKMLTSVH
                     REVPAGQPWPLPQTALNMSFLFTGAAGTPTLEHELTKEMRSADRLDLLVSFIKLSGLR
                     LLMPALQELTKRQVPVRVITTSYMGASDPEAIEWLVQCPNVRLKVSYDTERTRLHAKA
                     YHFYRQSGFSTAYIGSANLTRVAITSGLEWTVKITEQDQPAVMQQFRGEFEGYWSSEA
                     FETCQKADLEKFRRAICGARAKEKEAGIRFFAEITPRPFQERILEKLRAEREVHKSYR
                     NLIVAATGTGKTVISALDYKDFCGTKLPRPKLLFLAHRVEILEQACDCFRTVLRDYNF
                     GDILGGVHEPAATDHLFCTIASANSRSLVRGLPKDYYEYIVVDEAHHGAAPSYQPLYE
                     HFTPQIWLGLTATPERMDGKSILPEYNYRIAAEIRLPEALEERLLCPFHYFGITDPVS
                     LDDDKFWRNGQYRVDALTNIYTGDDIRAWQRVEALFSAFEEYKLFGDRTRAIGFCASV
                     KHAHYMKDVFLERGIQADTLLGETEGNRRSEVVQAFRNGEIQALFTVDVLNEGFDLPE
                     INLVMFLRPTDSLTIFLQQLGRGLRHAPDKDCLTVIDLVGQQHRSCRIDRKFAALLPR
                     QRMRIDEEAEHDFPHLPPGCNIYIQPVARRQILQHIRQTLQDIQRFTLESLATMSAQR
                     GRTPSFEEFIHETQVDPIELLRKDTWSAWKARAGLQPPTKDPDQSDLHKAMVRLATRN
                     SPTLLENVRREAQFALGESATVYQPPKPAEAIMLHYLLRNRPALARSEPEKTLHLLQN
                     NPNMARDIDEIAAWRQHVSDIVPFRPELPFDCPLDLHAAYGYEEIKAALGVATMETAG
                     PTGTGVIHAKHLRAYLHFVTFNKSEKDFSTTNRYQDYLLNRTQLHWESMSTTTQESAT
                     GQNYIHFAERGYTILFFARLNKKDGRIICPFIYLGPASALLRYEGNRPIRMIWELKHP
                     VPAEMYELNGRGG"
     gene            4464..4592
                     /locus_tag="PKW60_07445"
     CDS             4464..4592
                     /locus_tag="PKW60_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99307.1"
                     /translation="MKKRQEVHPTEVILMGSRVQWHQSPDFDLELLEDFAAQRLLR"
     gene            complement(4953..>5984)
                     /locus_tag="PKW60_07450"
     CDS             complement(4953..>5984)
                     /locus_tag="PKW60_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPJ99308.1"
                     /translation="DPDIERSAQSAHNYCRELSALVRHFGNHPSIIMWVPFNEGWGQF
                     QTSEITDWLKQLDPSRLVNQTSGWSDRGGSDVYDKHSYPGPDMFPIEDHRASVLGEFG
                     GLGWPVEGHLWWDKRNWGYRTYQDRETLARHYELLIRRLRYLIHDGLAAAVYTQTTDV
                     EGEVNGLMTYDRELVKLGADWLKRVNDTAYLPPPELITLAATSRDHGQTWRYTTDDPG
                     QGWEQPGFDDAGWKEGPGVLGRKDTPGANVRTDWLTPAIWARREFTIEAVPQGPCFLD
                     ILHDEDAEVYINGVLAIRVEGHIGSYDQFPLTGDARAALKPGANLIAVHCTQTEGGQS
                     IDVGLAAEK"
BASE COUNT         1193 a         1646 c         1813 g         1332 t
ORIGIN      
        1 cgcacgagcg cgtcgcagca tttccgcgcc gggccccgca ttcctttggt ttcattgccc
       61 atcaccacga cacagcgctc ggcccacgaa accgtaaaaa ccgactccct cccggcggga
      121 tccatcccgt acacccagaa tcctgcctcc tgcaactggc gcagggtgtc ggggaatttc
      181 gtggtcatga tgaccggcag atgaaacagt gcgcccgcgc tcgcgcgcac taccgactcg
      241 tccagcagcg ccccgccacg cgcggcgagg atcagcgcgt ggcatccggc tccgacggcc
      301 gtgcgaatca gcatccccag gtttttggga tgctggacgc cgtcgagggc caataccgtc
      361 gcctgcggag gacagctcgc gagaaacgcg tccaattcgg tataggcaac ggggctgatg
      421 cgcgccagca cgccctgatg atcgcgcgag ccggcgagct cgttgagcct ggccagcggc
      481 acaaaatcgt aggggatgcc ctgggtgcgg gccaggtcga ccagggcctg tttttcacgc
      541 actttggcgt ctttggcgag gtagagccgg ttgacgcgcc cctgcgcgcg cagggcctcg
      601 cccacggcat tagtcccgaa gattattccg ggcacggcgc ccggttccgc atggtcactc
      661 atccgtttcc ggcctccgtc gaagctcctt tttctcagac agcctgcgct tggaatcggc
      721 gcggcgcctg cgcgacgcgg cagagggccg ggtggcaacg cgcggcttcc ggcggcgtct
      781 gcgaagggca aggcggcgtt cgagctcctc cagcgcggcc gctttgttgc ggtgctgtga
      841 acgggattcg gaggccgtga cgaccagccc cgtcggaagg tgccgtaccc gaacggccga
      901 ctccgtcaca ttcttcttct gtccgcccgg accgcggctc ttataaaaac tgatctccag
      961 ctcatcatca cgaaaaggca tgcacagttc cttgccgccc gccgcaacca cctcccgccc
     1021 acctcccgcc catggtaccg gcaacaaagc ccgcacaaca agtgaagtgg gggagagtga
     1081 gtggtgagtc gacaaaacaa cggtcactca tctggagctc ggatgtcttc gataaagatg
     1141 ctcataaaag cgccggccct ttgcaataca gagcgggagg cctcataaaa acgcggatgc
     1201 ttaaccgccc cggccattaa gctcgtacat ttcagcgggt accgggtgtt tcaattccca
     1261 aatcatacgg atggggcggt taccctcata acgcagaaga gcgctggcag ggccgagata
     1321 gatgaacggg cagataattc ttccgtcctt tttgttcagc ctggcgaaga agagaattgt
     1381 ataaccacgt tcggcgaaat gaatgtaatt ttggccagtt gcgctttctt gtgtcgtggt
     1441 ggacatgctt tcccaatgca gttgcgtgcg gttcaacaaa tagtcctgat agcggttggt
     1501 cgtgctgaag tctttctcgg atttgttgaa ggtgacgaag tgcagatatg cgcgtaaatg
     1561 tttcgcatga atgacgccgg tgccggttgg tccggcagtt tccattgttg cgactcccag
     1621 cgcggcctta atttcttcgt agccgtacgc tgcatgcaaa tccagcgggc agtcaaacgg
     1681 cagttctggt cggaagggca caatgtccga tacgtgctgg cgccacgcgg ctatttcgtc
     1741 gatgtcgcga gccatgttgg ggttgttctg caacagatgg agggtctttt caggctccga
     1801 ccgtgccaaa gccgggcgat tgcgcaacaa gtagtgaagc ataatcgctt ccgcaggctt
     1861 aggaggttga tagacagtcg ctgattctcc aagagcaaac tgggcctctc gcctgacatt
     1921 ctcgagcaga gtggggctat tgcgggtcgc gagcctcacc atagctttat gaagatccga
     1981 ttgatcggga tcttttgttg ggggttgtaa accagcacgc gctttccaag cgctccacgt
     2041 atctttgcgg agcaattcaa tggggtcgac ttgagtttcg tgtatgaact cttcaaaaga
     2101 aggcgtgcgg ccgcgctggg ctgacatcgt ggcaagagac tcgagggtga atctttggat
     2161 gtcttgcaac gtctggcgaa tatgctgaag aatctgtctt cgtgctacgg gttgaatata
     2221 gatgttgcac ccgggcggca gatgaggaaa gtcgtgttcg gcctcctcat caatacgcat
     2281 gcgttgccgc ggcaatagtg cggcgaattt tcgatcgatt cgacagcttc ggtgttgttg
     2341 gcctacaaga tctatcacgg tcaggcagtc tttgtcgggg gcatggcgaa gcccacgccc
     2401 cagctgctgc aaaaagatcg tgagactgtc ggtgggacgc agaaacatca cgaggttaat
     2461 ttcgggcaaa tcgaaccctt cgttgaggac atcgacagtg aagagcgctt ggatctcgcc
     2521 gtttctgaag gcttggacta cctccgagcg gcggttccct tctgtctccc ccaacaacgt
     2581 atccgcctgt attcccctct caagaaagac atctttcatg tagtgagcat gttttacgga
     2641 tgcacagaat ccaatcgcgc gtgtgcgatc tccaaacaac ttatattcct cgaaggcact
     2701 gaatagcgcc tcgacgcgtt gccacgctcg gatgtcatcg ccggtgtaaa tattggtcaa
     2761 tgcatccacg cggtattggc cattacgcca aaatttgtca tcgtcgagcg atacggggtc
     2821 agtaatgccg aagtagtgaa atgggcacaa gaggcgctcc tcgagtgctt ccggcaggcg
     2881 aatctccgcg gcgatccgat agttgtactc ggggaggatg gattttccgt ccatgcgctc
     2941 tggcgtagcc gtaaggccga gccagatctg cggcgtaaaa tgctcataaa gaggttggta
     3001 cgagggtgcc gcgccgtggt gcgcctcatc aacgacgatg tattcatagt agtctttggg
     3061 gagtccacgg acgagactac gcgaatttgc gctcgcaatt gtgcaaaaga gatgatccgt
     3121 ggccgcgggc tcgtgcacgc cccctaagat gtcgccgaaa ttgtagtctc tcagaacggt
     3181 cctgaaacaa tcgcaggcct gctccagaat ctctacgcga tgggctaaaa acagaagctt
     3241 tgggcgcgga agcttggtgc cgcaaaagtc tttgtaatcc aatgcggaga taacggtctt
     3301 gcccgtgccg gtagcagcaa cgattaaatt tcgataactc ttgtgcacct cacgctctgc
     3361 acggagtttc tccaagatcc gttcttggaa agggcggggg gtgatctcag cgaagaagcg
     3421 aatccccgct tccttttctt tggctctggc accgcaaatt gcgcgtcgaa acttctcgag
     3481 gtccgctttc tggcaggttt caaaagcctc actgctccag tagccctcaa attctccgcg
     3541 gaattgctgc atcactgcag gttgatcctg ctcggtgatc ttcacggtcc attccaatcc
     3601 gctggtaatg gcgacacgcg tgaggttcgc cgagccaatg tatgccgtgg aaaagcccga
     3661 ttgccggtag aaatggtatg ccttagcatg caggcgcgtg cgttctgtgt cgtatgagac
     3721 ctttaaccgc acattggggc attgcaccag ccactcgatg gcttccggat cagaggcgcc
     3781 catataagag gttgttatca cgcgcacggg cacttgacgc ttggtgagtt cctgcaacgc
     3841 aggcatcaag agtcgtaatc ccgaaagttt gatgaaggac acgagcagat ccaatcgatc
     3901 ggcgcttcgc atctccttcg tcagctcgtg ctccaaggtt ggcgttccgg ccgcgccggt
     3961 gaacagaaag ctcatattca atgctgtttg aggcaaaggc cagggttggc ctgctggaac
     4021 ttctcgatgg actgatgtaa gcatcttgag ggggtgcaag attcttttgc gaaccagata
     4081 gtcaaggcct tcctgcgcac ccaggagttc gatgaggcta ttcaagagat tagctcgcgt
     4141 tgcagactta cattgtcgca acgcttccag aaatacctgc ccgaggagca aattgaattg
     4201 ttcaggctct tcctcctctt cgagttttcg cagggttggc tgtaggcctg gtgaatgggc
     4261 gatcagatct ttcagctctt catcaagtat acgtgcgtac gcgcctggtg ccagcagagg
     4321 gtcactactc atgacaggcg cgcctgaatc tggtccacga tcggcaagtc tgcgggcgcc
     4381 caaggtaggg ttccaagagc ctccacccgg agccaccgtg cctccaaatg ctcagatagt
     4441 gtaatggttg gcgcctcgga gcaatgaaaa aacggcaaga ggtgcacccg accgaagtca
     4501 tactgatggg aagtagggtc cagtggcatc aaagtcccga cttcgatttg gagctcctcg
     4561 aggatttcgc ggcgcagcgc ctgctccggt gattcgcctg cctcgatctt gcctccaggg
     4621 aactcccaaa ggccacccag aggtttgtcg atagggcggc gcgtggcgag tatttcgccg
     4681 tgctcattct gtaacacaag acatactacc gagactgtag actgatacat gcgtacgaag
     4741 gatcctaggc gtatattgca ctgctaactg ccacagattg tagcagtcag ctcacttggc
     4801 tatctatccc agtgcaccaa ccaaacgcga gactaagaga gactccgacc cgttgtcaag
     4861 gattcgcacc tgaaggtttt gtgacatccg aggcgtaggc ggcctgctgg gcgttttcag
     4921 cggcgtggtg cgctggtgtg tgccgtggtg gcttattttt cggcggcgag gccgacgtcg
     4981 atgctttgtc ctccttcggt ctgagtgcaa tggacggcga tcaggtttgc accgggtttg
     5041 agtgcggccc gggcgtcgcc ggtgaggggg aactggtcgt agctgccgat atgcccttcg
     5101 acacggatgg cgaggacgcc gttgatgtag acctcggcat cttcgtcgtg gaggatatcc
     5161 aggaagcagg gcccctgggg cacggcttcg atggtgaatt cgcggcgggc ccagatggcg
     5221 ggcgtgagcc agtcggtgcg gacgttcgcg ccgggggtgt ctttcctgcc gaggacgccg
     5281 gggccttctt tccatcccgc gtcatcaaaa ccgggctgtt cccagccctg tccggggtcg
     5341 tcggtagtgt agcgccaggt ctgcccgtga tctctggagg ttgccgcgag ggtgatcagt
     5401 tcgggcggcg gcaggtaggc ggtatcgttg acgcgtttga gccagtcggc gccgagtttg
     5461 acgagttcgc ggtcgtaggt catgaggccg ttgacttcgc cttcgacgtc ggtggtctgg
     5521 gtatatacgg cggcggcgag tccgtcgtgg atgagatagc gaagccttcg gataagcagt
     5581 tcgtagtggc gcgcgagggt ttcacgatcc tggtaggtgc ggtatcccca gttgcgctta
     5641 tcccaccaga gatggccttc caccggccag cccagtccgc cgaattcgcc caggacggac
     5701 gcgcggtggt cctcgatggg gaacatgtca ggcccgggat agctgtgttt gtcgtagaca
     5761 tcgctgccgc cgcgatcgga ccagccactg gtctgattga cgaggcggct gggatcgagc
     5821 tgtttgagcc agtcggtgat ttcgctggtc tggaactggc cccagccttc attgaacggc
     5881 acccacatga tgatggaggg gtggtttccg aagtggcgga cgagtgcgct gagttcgcgg
     5941 cagtagttgt gagccgactg ggcgctgcgc tcgatgtcgg ggtc
//
LOCUS       DAOPBQ010000190         5947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11672_length_5947_cov_3.233707, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000190 DAOPBQ010000000
VERSION     DAOPBQ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11672_length_5947_cov_3.233707"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PKW60_07455"
     CDS             <1..241
                     /locus_tag="PKW60_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99309.1"
                     /translation="VAAKTPSLPRISRFVAQKVAPLAHKFGEEKMRDAFSICMDAEIL
                     LRSTHVDRALAMELLVTRLAAPVARQVRARRTADR"
     gene            308..745
                     /locus_tag="PKW60_07460"
     CDS             308..745
                     /locus_tag="PKW60_07460"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HPJ99310.1"
                     /translation="MRPFDHDYAEEIIERLSGLSPDARPRWGRLTQNQLHAHLADALR
                     YSMGRNGGPVDCSTWFTRRIMGPLVLSGWWRIPQKFQFSNYGELPEGNLETLQAVLED
                     YLNLVQAGELEPLPHSRFGQLGVDGWARFHVAHFEHHLRQFGV"
     gene            complement(790..2529)
                     /locus_tag="PKW60_07465"
     CDS             complement(790..2529)
                     /locus_tag="PKW60_07465"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPJ99311.1"
                     /translation="MPIITESAPEAADSDKPKILVGFGPGIAPETLLPEEYIIRTVDN
                     ALCIVGEQSSRTPLATFYGACDFIDRVLGVRWLWPGEIGTYVPRQATIAVAPLDIRTR
                     PALEKRGLRIHLRRGHEAQNVPPLVDAETLSRMEVEAEQWLQHHRMGERSTLRFGHAF
                     GSWWDRYHEAHPDYFAVPPTGKKQPYPNPGRVKLCVSNPAVAEQILEEWRAAGRPDNW
                     NVCPNDSRGFCTCPNCCALDGIPGQAPETVWAGEEAILTGRYIALWNALLTRMRAENP
                     NVTLSSYAYSNYREPLEGMHVEEGLVLGIVHTYHAYEPWRKWHEAGAKLFLRPNWWHI
                     GALAPCNPLHAMGEYFTFAYEHSMLGFDFDSLMGYWATQGPSYYLIARLSVHPSLSVD
                     TVIDEYCSAFGSAAPHISHYLAYWEDHTTKCGYALPAGGAVQTGAESLYLKTCEEHGF
                     SASPLRSGWLVLPYLYPDSVVTPAFKILDEAARAAEGDEAMVQARVRFLRDGLVHLEQ
                     TRDAIALVYDEHRPARISDADVAARLASLQALRAELTPRHVVWGEVANWVEFHRGVRS
                     AFGRPEWDAFREQ"
     gene            2592..2735
                     /locus_tag="PKW60_07470"
     CDS             2592..2735
                     /locus_tag="PKW60_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99312.1"
                     /translation="MSIGGQHNLGFRFTILDEHHGAGLGHRYAKKRRSQDRRHDTFRE
                     FTH"
     gene            complement(3000..3431)
                     /locus_tag="PKW60_07475"
     CDS             complement(3000..3431)
                     /locus_tag="PKW60_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99313.1"
                     /translation="MSLLVVTDTRRGRREWPVRFRGKGYQVESQEIGNGGLVLDPYAG
                     YAVILFHFTNLPAAQALAAVRMFHTCYAGARIVVIADLAAQCRAFQEAGATVHLAEPV
                     EPSLVFTVVEMLTLWVQAKRAARKPALRLVAEPTAFCATVS"
     gene            complement(3850..>5947)
                     /locus_tag="PKW60_07480"
     CDS             complement(3850..>5947)
                     /locus_tag="PKW60_07480"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99314.1"
                     /translation="NVVAVEAAGYNVNSYYIIDEPAFLQAEILNGDAVIAATGAEPPF
                     EATILDQRIQKVERFSFQRPFIEAWRLDPASYAWRTGADAAFEPCALAVTAEKKLLAR
                     HIPCPTFRARPALRVVKEGRLLPDQEAESPWPGHAGRIRPDYKGYPTEVLEVHPSQDI
                     QKLASVDAVAVDVPLAEGTRVELPENSFRILDLGLNLSGFLGATITCTSPVTVYFAFD
                     EILSQDDVHWARMGCAQFVPYYLAPGTYAVESFEPYTMRYVKVMAAGGACTIENAYLR
                     EYVYPDAYEATFAASNPQLATLFEAGRETFVQNSTDVFMDCPSRERAGWLCDSFFTAR
                     TALVLSGDTRLERNFYENFLLPERFEHLPDGMLPMCYPADHYNGNFIPNWAMWFVVEL
                     EEYAARSNDQAMVDALKPKVLALFDYFKKFENEDGLLEKLEQWVFVEWSEANKFVQDV
                     NYPTNMLYAGTLAAAGRLYAMPELLAKAERIRETIREQSFDGEFFVDNAVYKDGQREV
                     TRNRTEVCQYFAFFFDVASPDTHAALWEKLHREFGPDRSETKAYPEVHPANSFIGNML
                     RFELLSRYGLGSQITGESVGYLMYMAERTGTLWENVQEVASCNHGFASHICFTLYRDV
                     LGVYRFNPIAKTITLRFNDVGLDWCEGAFPVLDGMIHLRWDKSGDTLRYRLDTPAGYT
                     ITVENPTGLVLEPLGE"
BASE COUNT         1143 a         1795 c         1855 g         1154 t
ORIGIN      
        1 ggtcgcggcc aagaccccga gcctcccgcg aatcagccgc tttgtagccc agaaggtcgc
       61 gccgttggcg cacaagttcg gcgaagagaa gatgcgcgat gccttcagca tctgcatgga
      121 cgcggagatt ctgctgcgca gcacccacgt ggatcgggcc ctggcgatgg aactgctggt
      181 gacgaggctc gccgccccgg tcgcgcgtca ggtgcgcgca cggcggacgg cggaccgtta
      241 gccgggggcc tgcttcgcga accttgaggc gccaggctgg aatcctcggc aaggagcaga
      301 atttgggatg cgcccattcg accacgacta cgcggaagag attatcgagc gcctgagcgg
      361 cctttctccg gatgcccggc cgcgctgggg ccgcctgacg cagaatcagc tgcacgctca
      421 tctggcggac gcgctgcgct attcgatggg ccgcaatggc ggcccggtgg actgcagcac
      481 gtggttcacc cgcagaatca tgggtccgct ggtgttgtcc ggctggtggc gtatccccca
      541 aaaattccag ttcagtaact acggcgaact gccggaggga aacctcgaaa cgcttcaggc
      601 ggtgctggaa gactatctga acctggtgca ggccggtgaa ctggagccgt tgcctcacag
      661 caggtttggc caactcggcg tggacggctg ggctcgtttc cacgtggccc attttgaaca
      721 ccatttgcgc cagttcgggg tctgacagcg gttttcggga tgcgcctggc gccggttcgc
      781 agcggttttc tattgttccc ggaaggcgtc ccattccggg cggccgaacg cggagcgcac
      841 gccacggtgg aactcaaccc agtttgcaac ttccccccac accacgtggc ggggggtgag
      901 ttcggcccgc agagcctgca atgaggcaag acgggcggcc acatctgcat cggagatccg
      961 ggcgggccga tgctcatcat agaccagcgc tatggcgtcg cgagtttgtt ccaggtggac
     1021 gagcccgtcg cgcaggaagc gtacacgggc ctgcaccatg gcctcgtcgc cctccgcggc
     1081 acgcgcggcc tcgtccagga tcttgaacgc gggcgtcacc acgctgtcgg ggtataagta
     1141 cggaagtacg agccatccgg aacggagcgg actggcgctg aaaccgtgtt cttcgcaggt
     1201 tttcagatac aggctttcgg cccctgtctg cacggcgccg ccggcgggga gggcgtaacc
     1261 gcatttcgtg gtgtggtctt cccagtaagc gagatagtga ctgatgtgtg gggcggcgct
     1321 gccaaaggcg gagcaatact cgtcgatcac agtgtcgacc gaaaggctgg gatgcaccga
     1381 caggcgggca atcagataat agctcggccc ctgagtggcc cagtatccca tgagcgaatc
     1441 gaagtcgaaa ccgagcatgg aatgctcgta cgcgaacgtg aagtattcgc ccatggcgtg
     1501 caggggattg cagggcgcca gcgcgccgat gtgccaccag ttcgggcgga gaaagagttt
     1561 tgcgccggct tcgtgccatt tccgccaggg ttcgtaggcg tggtaggtgt ggactatccc
     1621 caggacgagc ccttcctcca catgcatgcc ttcaagcggt tcgcggtaat tgctgtaggc
     1681 ataggatgac agggtaacgt tggggttttc ggcgcgcatt cgggtcagga gtgcgttcca
     1741 caacgcgata tagcgccctg taagaatcgc ctcttcaccc gcccagaccg tttcaggggc
     1801 ctggccgggg atcccgtcaa gggcgcagca attggggcag gtgcagaagc cccggctgtc
     1861 gttggggcac acgttccagt tgtcgggccg acccgcggcg cgccattcct ccagaatctg
     1921 ctctgccacg gccgggttgc tcacgcagag tttgacacgg ccgggattgg ggtacggctg
     1981 ttttttcccc gtggggggca cggcaaaata gtcgggatgg gcctcgtggt agcgatccca
     2041 ccagctgccg aacgcatggc cgaaccggag cgtgctgcgt tcgcccatcc tgtggtgctg
     2101 cagccattgc tccgcctcca cctccatgcg gctcagcgtc tcggcgtcca ccagaggcgg
     2161 cacattctgg gcttcgtggc cgcgccggag atggatgcgc aaaccgcgct tttccagggc
     2221 cgggcgcgtg cggatatcga gcggcgctac ggcaatcgtg gcttgtcttg gaacgtaggt
     2281 gccgatttcg cccggccaca gccaacgcac cccgagcacg cggtcgatga agtcgcaggc
     2341 gccgtagaat gtcgccaggg gagtgcggga tgactgctca cccacgatgc acagcgcgtt
     2401 gtcaacggtc cggatgatgt actcttcggg caggagcgtt tcgggggcga taccgggccc
     2461 aaacccgacc aggattttcg gtttgtccga atcggctgcc tcgggagcgg attcggtgat
     2521 aatgggcaat gccgcgccgg aagcgcgctc gatgtggtag cggagttcgt gcgcagcgcg
     2581 ctggacctcg ggtgagcatc ggcgggcagc acaatctcgg ctttcgcttc accattctcg
     2641 acgagcacca cggggccggc ctgggccacc ggtatgccaa aaaacgccgc tcccaagacc
     2701 gccgccacga cacatttcgt gaattcacgc attgaaaacc gctccttgtg aaagtgcgtt
     2761 atcgtgaatg gccccttgcg cgcattatag tggcccgtgg ctgcatggac aaccgtctgg
     2821 ccaggaatgc gtttggggtg gacatggccc gcgctgtgga gggtacgtct gttcatacgc
     2881 aggccgggga tggaatgcat gggggaaaat cgggctctgc ctcgcgggaa tcccgacctt
     2941 tccgcgggta cgaatgctcg agcgcgagga gcggatacgc gtgtgcccga gtgccggcat
     3001 caggagaccg ttgcacagaa ggccgtcggc tccgccacga ggcgcagcgc gggttttctc
     3061 gccgcccgtt ttgcctgaac ccacagcgtg agcatctcca ccactgtaaa taccagggaa
     3121 ggctcaacag gctccgccag atggacggtg gcgcccgcct cctgaaaggc gcggcattgc
     3181 gcggccagat cggcgatcac gacgatgcgc gcacccgcgt aacaggtgtg gaacattcga
     3241 accgcagcca gtgcctgcgc cgcgggcaag ttggtgaagt ggaacaggat tacggcatac
     3301 ccggcataag ggtcaagcac cagcccgccg ttcccgattt cctggctttc aacctggtac
     3361 cctttgcctc gaaatcgtac cggccactct cttcgcccgc gccgtgtgtc tgttacaacc
     3421 agtaagctca tgggcagacc tcccttggcc cgcgccgcca cactccccgt gacgccttcg
     3481 cttctggcaa tacgaccgtc agatctgtac ttggacgcga aatctcccgc agcgtgagtg
     3541 tcactatccc acaagacgcc agatcgcaca ataggtgagt tgggctctgc cggcataagg
     3601 gaattcccta tatggatcgg acggattcct cgataccgct caattcactg gaaaacgctg
     3661 caaaaaaatg gtccatttca ggatgaaatc gctaaaccac gtgagatctg gctcttacgg
     3721 tgcaggtatc tcatcggcac ctcaattgtc ctgaaagatg aggctccccg agaggcgtct
     3781 gcgcagcgct gtaacggcag atagagaaag ggcgcccttt gtgtcgagcg ccctccgttg
     3841 ttacgagatt tactcgccca gcggctccaa aaccaggccg gtaggattct cgacggttat
     3901 ggtgtagcca gccggggtgt cgagccggta gcgtaatgtg tctcccgact tgtcccagcg
     3961 cagatggatc atgccgtcga gcaccgggaa ggctccctca caccagtcca gaccgacatc
     4021 gttgaagcgc agcgtgatgg tttttgcaat ggggttgaag cggtatacgc ccaacacgtc
     4081 ccgatacagg gtgaagcaga tgtgcgaggc aaacccgtgg ttgcagctgg ccacttcctg
     4141 tacgttttcc cagagcgtgc ccgtgcgctc ggccatatac atcaggtaac ccacggattc
     4201 gccggtgatc tgtgagccca gtccatagcg cgacagcagt tcaaaccgca acatattgcc
     4261 gataaatgaa ttcgccggat gcacctcggg ataggccttg gtctcgctcc gatccgggcc
     4321 gaactcccgg tgcagttttt cccacagtgc agcatgggta tccggggatg ccacatcaaa
     4381 gaagaacgcg aaatactggc agacctccgt ccggtttcgt gtcacctcgc gctggccgtc
     4441 cttatagacc gcattgtcta cgaagaactc gccatcaaag gactgctccc ggatggtctc
     4501 gcggatacgt tcggctttgg cgagcagttc cggcatagcg tacaggcggc cagctgccgc
     4561 aagggtgccc gcgtagagca tgttggttgg gtagttcaca tcctgcacga atttgttggc
     4621 ttcagaccat tccacgaaca cccactgctc gagtttctcc agcaggccgt cctcgttctc
     4681 gaattttttg aaatagtcaa acagcgccag gactttgggc ttcagcgcgt cgaccatggc
     4741 ctgatcgttg ctgcgcgccg cgtactcctc cagttccacg acaaaccaca tcgcccagtt
     4801 tggaatgaag ttgccgttgt agtggtctgc gggatagcac atcggcaaca tgccgtccgg
     4861 gaggtgctca aagcgttccg gcaacagaaa attctcgtag aaattgcgtt ccagccgtgt
     4921 gtcgccgctc agcaccagag ccgtgcgcgc cgtaaaaaag ctgtcgcaca accatcccgc
     4981 gcgctcgcgc gacgggcagt ccatgaacac gtcggtgctg ttttgaacaa aggtttcacg
     5041 cccggcttca aacaaggtcg ccagctgcgg gttgctcgcc gcaaacgtgg cctcgtaggc
     5101 gtcagggtag acgtactcgc gcaggtaggc attctcgatg gtgcacgcac cgcctgcggc
     5161 cattaccttg acatagcgca tcgtgtacgg ttcgaacgac tcgaccgcgt acgtgcccgg
     5221 cgccaggtaa tagggtacaa attgcgcgca acccatgcgc gcccagtgca cgtcatcctg
     5281 gctcaggatc tcatcaaacg cgaaatacac cgtgaccggt gacgtgcacg taatggttgc
     5341 gcccagaaat ccgctcagat tcaagccgag atcgagaatg cggaacgaat tttccggcag
     5401 ctccacgcga gttccttccg caagcgggac atcaaccgca acggcatcca cgcttgcgag
     5461 tttttgaatg tcctgcgaag gatgcacttc aagcacttcg gtggggtagc ccttgtagtc
     5521 gggtctgatg cggcccgcat ggcccggcca gggcgactcg gcttcctggt ctggaagaag
     5581 ccggccttcc ttgactacgc gcagtgcggg ccgtgcgcgg aaggtggggc aggggatatg
     5641 ccgcgccagc agctttttct ccgctgtgac ggccaatgca caaggttcga aggccgcgtc
     5701 cgcaccggtg cgccaggcgt aactggccgg atcgagccgc caggcttcga taaacggccg
     5761 ctgaaaactg aaacgttcca ccttctgaat tcgttggtcg aggatggtcg cctcaaaagg
     5821 cggttcggcg cccgtcgctg caatcacagc atcgccgttc aggatctccg cctggagaaa
     5881 cgcgggttca tcgatgatgt agtagctgtt gacgttgtag cccgccgctt cgacagccac
     5941 gacgttc
//
LOCUS       DAOPBQ010000191         5910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11740_length_5910_cov_4.659607, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000191 DAOPBQ010000000
VERSION     DAOPBQ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11740_length_5910_cov_4.659607"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..519)
                     /gene="flgB"
                     /locus_tag="PKW60_07485"
     CDS             complement(124..519)
                     /gene="flgB"
                     /locus_tag="PKW60_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HPJ99315.1"
                     /translation="MEPFAGVDVTRVLHNAMRVAATNHRIIANNVANADTPNYNAVRM
                     DFQATLRAALEGRDRVSLRKTQARHLDSVRYFAQFDNLAISSKNDYNKVDLEEEMARL
                     SANTNDYTTYGSLLVKYFQQTKNMLNTLR"
     gene            complement(823..2199)
                     /locus_tag="PKW60_07490"
     CDS             complement(823..2199)
                     /locus_tag="PKW60_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPJ99316.1"
                     /translation="MARSQILVIDDEPLMGDYVQEALIRAGHDVDVCTSGPAGLELMR
                     KNSYDLLFTDLRMEPMNGIEVLQQARKESPGTHCIIMTAYATVETAVAALKEGAVDYV
                     LKPFEPDALELAVSRVLQREQLARENRYLRAEVTERYDFDRMVGGSAAISRVYESIRR
                     VADSRATVLIRGESGTGKELVARALHYKSSRRDMPFIKVNCAALSAGLLESELFGHEK
                     GAFTGALERKIGRFELADTGTLLLDEVSEISPELQPKLLRALQEREFERVGGNRAIHV
                     DTRIICTTNRDLERAMREGRFREDLFFRLNVVPIHLPSLRERREDIPALMEFFLNRFV
                     RENARPVRGFAPEAARLFMEYDWPGNVRELQNAVERAVVLSTDAVLGPEHFALGAGVP
                     REASSGNLVSVPVGTTVGEMEKILILRTLEQSAQNRTRAAEVLGISVRTLRNKLKEYG
                     QNSADDDE"
     gene            complement(2266..3429)
                     /locus_tag="PKW60_07495"
     CDS             complement(2266..3429)
                     /locus_tag="PKW60_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPJ99317.1"
                     /translation="MRENSDTDKMQALEHAFAEFTRTTAAMGEWYRSLQARVDELDRE
                     LEDKNRQLALANEYLSYILESMSDGVIAVDTAGCITRFNRAASEVLGYAAEDVVGKSF
                     RDVFGREFAAPPGGRHPMELRTRNGRTFPVSERDAPIADRNNQRIGTVKVFEDLSEIE
                     TLRQRVRQKDRLAALGEMAATVAHEIRNPLGGLKGYAALLARDLPGEDPRARLVSKVI
                     DGANQLELVVSDLLEYTRPVLLRLEEISCAETTEAALRYLGEIPAGIQVLNQVGEDHR
                     VVADGMKLRQALLNILQNAVQSIDGPGEVAIASEQDATSTVFTIRDTGRGIQPGLLEK
                     VFSPFYTTREKGTGLGLAVAAKIVEAHGGKIWAESCEGRGSQFHIRLTRAGGS"
     gene            complement(3426..3866)
                     /locus_tag="PKW60_07500"
     CDS             complement(3426..3866)
                     /locus_tag="PKW60_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99318.1"
                     /translation="MTDHSIAARLADCFQRQLALFERMAEAHASLPGDPESREWAELL
                     RIQTEFSRDLAALEQEFQILKREWNRAPDAEPDARSQLRELAEAARHKAGEIARASAA
                     NAEQVAEMMRNAREQLAEIQKGKRILRNLRAGEQDGADYIDRKA"
     gene            complement(3863..>5910)
                     /locus_tag="PKW60_07505"
     CDS             complement(3863..>5910)
                     /locus_tag="PKW60_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ99319.1"
                     /translation="TFLSSYTSRVPELDVPSVTRTRDLESVLEQAKAAFATGSYAAAA
                     RLLEAPLRAAPPSPLRIEAELLRLEAAYRALPPHAPEFEIEIVNSYVDTFLAQAPSHA
                     RAPEVLQWKADLYERAGVPIAACNVYADLLADYPDLPNRPELLLRAGRLALDTGRYAE
                     ASTHLEQLVKTHPGTPEAADGTLLLGEALVHLGKQAEGEALLTRVAQTNAQNRLGAGA
                     CTALGRIALDRGDYQTAITLLKTRLQSGTTSVGNDEVLLMLAQAFRAAHQIQDAQDAL
                     RDIIRFFPGSASSPQAYLELTETLDMAGRRDDALRIASEATQRHPGNPEILLRYAQLR
                     ELTGDTRGAAQVLLAAEQAGASDPQVLLTAGRQFLKAGYADEAETAFERLASEFPGTP
                     QSIAANVALAKLAFKRHESGRAMALLQDMAALAQGTPQETEVMLAQGEMYQELGLLDQ
                     AAGIYRGVAARAPESEVLAQAARALYRTGAWAEGNEVARRVDVEQLAPATAYAFLMEY
                     GHAMRLADSGQALRLMEQAHTDFPGERTLDYTRQLVETCLALDRFEQAMAIAIEFFAT
                     ADGARAENCRELLIACGDYAMEHQDHAQAAACYSRILEKNAPDTRNVQWARFQLANAL
                     QDLGKLDEARPLLTAIAASTSPWAAEAQLKLEYLGLRARMGVDGVSETPKRTGGPAP"
BASE COUNT         1029 a         1840 c         1891 g         1150 t
ORIGIN      
        1 ggcgttggca atattgttcg cgatgacgtt catccgcgtg cgctgggcct tcaggccgcc
       61 caccgcgata tccgtggccg agaccgagcc aaacatcgtg ccgtcaacca tggtgcttgc
      121 gtcctatcgg agcgtattga gcatgttctt ggtctgctgg aaatacttga caaggagact
      181 accatatgtg gtgtagtcgt tggtgttggc actaagcctt gccatttcct cctccaagtc
      241 taccttattg tagtcatttt ttgacgaaat cgcaagatta tcgaattgtg caaaataccg
      301 cacgctgtcg aggtgccgag cctgggtctt gcgaagagag acgcggtccc ggccctcgag
      361 cgccgcccgg agggtggcct gaaaatccat gcgcacggca ttgtaattgg gcgtgtcggc
      421 gttggcgacg ttattggcga tgatgcggtg gttggtagcc gccacacgca tcgcgttgtg
      481 cagcacgcgc gtcacatcga cacccgcaaa cggttccaac gaagtcctcc tttctctgca
      541 agccatttcg tcgcttgacg ttagagcaaa gttcatgcca tggaagacaa acagcctaag
      601 tgatttaaaa ataatgcgtt gcaaaccagg cgcacgaaat gaaaaatgcc tgatcggggg
      661 aaataaatcc ccccgtgcgg aggaatctgc ccagttgtgg tttgaagaag cacccggcgg
      721 gcatggggca cgtatcagcg ctgtgaagtg ggaacgccga cctatcccgg gcagcgcccg
      781 cggcgtggca gcccctgtcc ggtcgaacag ccggcgacgg ggttattcgt cgtcatcggc
      841 gctattctgg ccgtattctt tcagcttatt acgaagcgtc cgcacgctga tgcccaggac
      901 ttccgcggcg cgggtccggt tctgggcaga ttgttccagc gtgcggagga tcagaatttt
      961 ttccatctcg cccacggtcg tgccgaccgg gacactcacc agattgccgc tcgacgcttc
     1021 gcgggggacg cccgcgccca gcgcgaagtg ttccgggccg aggacggcgt ccgtagacag
     1081 cacgaccgcg cgttcgacgg cattctggag ttcacgcaca tttccgggcc agtcgtattc
     1141 catgaacagg cgcgccgcct ccggggcgaa cccgcgaacc ggccgggcgt tttcacggac
     1201 gaaacggttg aggaagaact ccatcaaggc ggggatatcc tcgcgccgtt cgcgcagcga
     1261 tggcagatgg atcgggacga cgttgaggcg aaagaacaag tcttcgcgga aacgcccttc
     1321 gcgcatggcg cgttccaggt cgcggttggt ggtgcagatg atccgtgtat cgacgtgaat
     1381 ggcccggttt ccgccgacac gctcgaattc gcgctcttgc agtgcgcgca ggagcttggg
     1441 ctggagttcg gggctgatct cgctgacttc atcgaggagc agggtgccgg tgtcggccag
     1501 ctcaaagcgg ccgatcttgc gttccagcgc gcccgtaaac gcgccctttt catggccgaa
     1561 gagttcgctc tcaagcagcc ccgcggacag ggcagcgcag ttgaccttga tgaacggcat
     1621 gtcccgccgc gagcttttgt agtggagcgc acgggcgacc agttccttgc cggtaccgct
     1681 ttcgccccgg atgagcacgg tagcgcggct gtcggccacg cggcggatgc tttcgtacac
     1741 acgggagatc gcggcgcttc ctccgaccat tcggtcgaaa tcgtagcgct cggtcacttc
     1801 ggcccggaga taacggtttt cacgcgccag ctgttcgcgc tgcaagaccc gcgataccgc
     1861 gagctcgagc gcgtcgggct cgaacggttt caggacatag tcgacggcgc cctccttgag
     1921 cgcagcgacc gccgtctcga ccgtggcgta ggcggtcatg atgatgcaat gcgtccctgg
     1981 gctttctttt cgggcctgct gcagcacctc aatgccgttc atgggttcca tgcggagatc
     2041 ggtgaagagc aggtcgtatg aattcttccg catcagctcg agcccggcag gtccgctggt
     2101 gcatacgtcg acatcgtgcc ccgcgcggat caacgcctcc tggacgtaat cgcccatgag
     2161 cggttcgtcg tcaattacca aaatctgcga tcgtgccatg ctatgcgctc ccggaaccgg
     2221 tctgtcgtga gacagcattc cgtgtagaaa ccagcgcgcg ggcggtcagg acccgccggc
     2281 gcgcgtcagc cggatatgaa actgggagcc gcggccctcg cagctttcgg cccagatttt
     2341 ccccccatgc gcctccacga tcttggcggc tacggccagg cccagcccgg tgcctttctc
     2401 gcgggtggta taaaacggcg agaacacttt ttccagcaag ccgggctgga taccgcggcc
     2461 cgtgtcgcgg atggtgaaca ccgtggaggt ggcgtcctgc tcggatgcaa tggcgacctc
     2521 gccggggcca tcgatgctct ggactgcatt ctgcaggatg ttgaggagtg cctgccggag
     2581 tttcatgccg tccgcgacca cgcgatggtc ttcgccaacc tggttgagca cctggatgcc
     2641 cgccggaatc tcgccgagat agcggagcgc ggcttccgtt gtttcagcgc agctgatctc
     2701 ctcgagccgg agcaggacag gccgcgtgta ttcgagtaaa tcggagacca cgagttccag
     2761 ctggttcgct ccatcgatga cctttgagac cagccgggcg cgcgggtctt caccggggag
     2821 gtcgcgtgcg agcagggcgg cgtagccctt caggccgccc agcggattgc ggatttcgtg
     2881 cgcaacggtc gccgccatct cgcccagggc agccagccgg tccttctgac ggacccgctg
     2941 acgcagggtc tcgatttccg acagatcttc aaagaccttg accgtcccga tgcgctggtt
     3001 gttgcgatcc gcgatcggcg cgtcacgctc gctcacggga aaggtgcggc cattccgggt
     3061 tcggagctcc atgggatgcc ggccgccggg cggagcggcg aattcccgtc caaacacgtc
     3121 gcggaaagat tttccgacaa catcctcagc ggcatagccc agaacctcgg acgcggcgcg
     3181 gttgaagcgg gtgatgcaac cggcggtatc gacggcgatc acaccgtcgg acatgctctc
     3241 aagaatataa ctcaggtact cattggccag cgcgagctgc cggttcttgt cctcgagctc
     3301 ccggtccagt tcgtccacgc gcgcctgcag cgagcggtac cactctccca tggcggcggt
     3361 ggtccgggta aactcggcga aggcatgttc aagcgcctgc attttatcgg tatcgctgtt
     3421 ctctctcatg ccttcctgtc gatataatcg gcgccgtcct gttcacctgc gcgcaggttg
     3481 cgcaaaatgc gtttgccttt ctggatctcg gccaattgtt cccgtgcatt ccgcatcatc
     3541 tccgcgacct gttccgcatt ggcggcgctt gcccgcgcga tctcgcccgc tttgtgtcgg
     3601 gctgcttcgg ccagttcgcg gagctggctg cgcgcgtccg gctcggcgtc gggcgcgcgg
     3661 ttccattcgc gtttgagaat ctggaactcc tgctctaggg ccgccaaatc gcgcgaaaac
     3721 tcggtttgaa tgcgcaaaag ctccgcccat tcgcgtgact caggatcgcc gggcagcgac
     3781 gcatgggcct cggccatgcg ctcgaacaag gcgagctgac gctggaaaca gtcggcaagg
     3841 cgtgccgcaa tcgaatggtc cgtcatggcg caggccctcc ggtccgcttg ggcgtttcag
     3901 aaaccccgtc cactcccatc cgggcacgca accccaggta ctccagtttc aactgggcct
     3961 cggcagccca gggcgatgtc gatgccgcga tcgcagtgag caaaggccgg gcttcatcga
     4021 gtttgcccag atcctgaagg gcgttggcga gctgaaaccg ggcccattgc acgttccggg
     4081 tatccggcgc atttttttcg agtatccggg agtagcaggc ggccgcttgg gcgtgatcct
     4141 ggtgttccat ggcgtaatca ccgcacgcga tcaagagctc ccggcaattc tctgcacggg
     4201 cgccatcggc agtcgcaaag aattcgattg cgatcgccat ggcctgctcg aagcgatcca
     4261 gggcgaggca ggtctcgacg agctgccggg tgtagtccag cgtacgctcc ccggggaaat
     4321 cggtatgggc ctgttccatc aagcgcaagg cctggcctga atcggccagt cgcatggcgt
     4381 gaccgtattc catgaggaaa gcatacgccg tggcgggggc cagctgctcc acatccaccc
     4441 ggcgcgcgac ctcgttcccc tcagcccacg ccccggttcg gtacagcgcc cgggcggcct
     4501 gggcgaggac ttccgattcg ggtgcccggg cagcgacccc gcggtagatg ccggcggcct
     4561 ggtcaagaag cccgagctcc tggtacattt cgccctgggc cagcatgacc tcggtctcct
     4621 gcggcgtgcc ctgggccaga gcggccatgt cttgcaggag cgccatggcc cgaccagact
     4681 catggcgttt gaacgcgagc ttcgccaaag cgacattggc agcgatcgac tgcggggtcc
     4741 cggggaattc ggaggccagt ctttcgaacg ccgtctccgc ctcgtccgcg taccccgcct
     4801 tgagaaactg gcgtccggcg gtcagcagga cctgcgggtc ggaagcgccg gcctgttcgg
     4861 ccgccaggag cacctgggcc gcgccgcggg tatcgccggt cagctcccgc aattgcgcat
     4921 aacgcaacag gatctcggga ttgccgggat gcctttgagt ggcctcggaa gcgattcgca
     4981 gggcatcgtc gcgccgccct gccatatcca gcgtttcggt cagctccaga tacgcctggg
     5041 gggacgatgc gctgccgggg aaaaaccgaa tgatatcgcg cagggcatcc tgggcgtcct
     5101 gtatctggtg cgcggcccgg aaggcctggg ccagcatgag gaggacttcg tcgttgccga
     5161 cgctcgtggt gcccgactgc aggcgcgtct taagcagcgt gatcgcggtc tgatagtcgc
     5221 cgcggtcaag ggctatgcgc cccagggcgg tacaggcacc cgcccccaga cggttctgcg
     5281 cattcgtctg ggcgacccgg gttagaagcg cctcaccctc ggcttgcttg ccgagatgga
     5341 ccagggcctc tcccaggagc aaggtgccat ccgcggcctc cggcgtgccc ggatgggttt
     5401 tgacgagctg ctcgagatgc gtggaggcct cggcataccg gccggtatcc agggccagac
     5461 ggcccgcgcg caacaagagc tccggcctgt tgggcaggtc aggatagtcg gcaagaagat
     5521 cggcgtacac attgcatgcc gcaatcggaa cgccggcgcg ctcatagaga tccgccttcc
     5581 attgcaggac ttccggagcc ctggcatgcg acggagcctg tgcgaggaac gtgtccacgt
     5641 agctgttgac gatttcaatc tcgaattccg gggcatgagg cggtagagcc cggtaggccg
     5701 cttcgaggcg aagcaattca gcctcgattc gcaatggact gggcggcgcc gcccgcaagg
     5761 gcgcctcaag caggcgtgct gccgcagcgt agctgcctgt ggcaaaagcc gctttggcct
     5821 gttccagcac tgattcgagg tcccgggtcc gcgtgacgct ggggacatcg agttcaggca
     5881 cgcgcgatgt atacgaggac agaaatgtgt
//
LOCUS       DAOPBQ010000192         5892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11786_length_5892_cov_3.855748, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000192 DAOPBQ010000000
VERSION     DAOPBQ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11786_length_5892_cov_3.855748"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2126)
                     /locus_tag="PKW60_07510"
     CDS             complement(<1..2126)
                     /locus_tag="PKW60_07510"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPJ99320.1"
                     /translation="MSRGVRLYPAAVVLVVLFLCGDGLAQEPQKTRSTLYSSEIVAAV
                     RRSTERFDWAAAARDRIVTSAQAWLNMPDDDLWELMFGATLPRSWMVWSNGYCPACKA
                     NVPMYSWKMAALAHSWKVQCPHCSEFFPTNDFEAFYASGLDEHHVFDPSRADRSLLFN
                     AAHPDADDPLHLFGVDDGTGYVEGEQRWRFIAAYLVYGQWKQGVVDGIRTLAAAHLMT
                     GDPVYARKAAILIDRVADLYPSFDFGEQGFVYERKADRGYVSTWHDACEETREIVMAY
                     DMIFEAIQDDPELTAFLSKKAVQYNLDNPKTTIADIQRNIETRILRDAITNRSRITTN
                     YPRTEIALAIIHAVLGMPENEAAFWEIVDPMLTKATAVDGVTGEKGLAGYSSYTISAL
                     GSFIGEFSKSDPSFLHVLLERHPSLRKTFLFFAHTLCLDRYYPQSGDTGAYCIPINRY
                     VGLNFTCYGNGSGGSGWTLIPPSMFTLCWEFYKETRDPLYAQIACRENGNGVSGLPYD
                     FYIDDPAPVVNDIEAVIQEHGEELDLGSVNFEQWHLALMRSGAGDDRRVLWLDYDSGG
                     GHGHTDGMNLGLFAKGLDLLPELGYPPVQFGGWESPRARWYTMSAAHNTVVVDGANSV
                     FGQAGETTLWAEGSIFHAMRMSAPGMIGGERFERTAILIDISPSDFYVVDLFHVKGGH
                     DHAKFLHSHFGELRLSGLSLTPSEDYG"
     gene            2516..3475
                     /locus_tag="PKW60_07515"
     CDS             2516..3475
                     /locus_tag="PKW60_07515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPJ99321.1"
                     /translation="MNVDIVIVNFNTCELLRECLVSISEAREEVDSMRTIVVDNASTD
                     ASVSTIRSEFPWVELVPLPENLGFGAGNNRGVARGQNEFILFLNSDAKLTPQALSRLA
                     SRLQASPLVVAVGPRLIYPDGSFQPSCRRFPTVWRWLWSTTGLASRFPRTFKSFRHWL
                     DEEEHVDGQAVDMVSGACFLMRRDYFESIGGFDENLFLYEEEFDVFYPAARRELEVGY
                     CGEATVIHGAGKSVEANRMAAFARFHLVRSKYYAFRKHYGRLAARLAYWVDLAVFGTT
                     GTLRKIRGKTAGALLTVRICRLAWPASRLTGTEVRSLKWDLPG"
     gene            complement(3491..5365)
                     /locus_tag="PKW60_07520"
     CDS             complement(3491..5365)
                     /locus_tag="PKW60_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HPJ99322.1"
                     /translation="MSNAYFCQLPDPGAMRTLAHSFSALLASGHERVNHYAESNGAIF
                     GLVSPDKFGQGAMPSYLEESGFWGLLVGELHPCAALDRCRHARPDAETDLALLASLAR
                     DKQLLATLPALNGAFFFMLWDPEARTLIAANDRYGLYPMYWAQKDGRYCLSSRTLGPV
                     LAGVVPGGIDTLAVAQILTVDDFVGGRTLVKDVSAFPPGTMMTKTPDRIDWQTYWVWD
                     YPAEQGLSAEEWGRRAGAALVEAVRRQAGAGRSIGVTLSGGLDSRCVAAAAVQTGISP
                     RTFTWGEDGCYDRLYASEIARALGIEHQDCPYEYDSLAERFEEGVAITEGMCNAFDMH
                     FLAHLHIIAGVDVVLNGFAGDAVLGETFLRPQWASPMRPAELAENLFHRYNRFLKEAE
                     LPEAMPVVRDFRQEDFPLSVFKQQFEELRHLGTRDAAHRFLFDNHVRRKTAMGTVLMR
                     QAAESAACFYDYDFNDLVRRIPAELRARHRTYRAMLRTVFPDVAAIRWQTTLLPPLAP
                     EWRILASKVMRRASAKVEAWAGWHGPTHRQAPALFAERLRGELRDWMDSLIRGEYPLP
                     DDILNPEFRRRMWQEHLDGKDHCRLLGALVPILELSRLVQRARNRQFASVPAPMLVQR
                     "
     gene            complement(5362..>5892)
                     /locus_tag="PKW60_07525"
     CDS             complement(5362..>5892)
                     /locus_tag="PKW60_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99323.1"
                     /translation="GAAMYVAGLLREAGAFPTEVAYGEEPLLCWRLRNILGRKIYQIN
                     QTMARHNLDFRGLRDYWRRNVRCGQTYAEIAAICWRTPDPLWRRECLTNAGWALLMFG
                     ALAGLIIGTTGLRVSILAVAGCVLARKFAQTRMKGHAAGVSLMYAVHSYFSKLSIAWG
                     ELRWVLRRDRRKVTGP"
BASE COUNT         1224 a         1788 c         1731 g         1149 t
ORIGIN      
        1 ccgtagtctt ccgagggcgt tagagacaga cccgacaggc gcagttcgcc aaaatggctg
       61 tgaagaaatt ttgcatggtc atggccgcct ttcacatgaa aaagatcgac cacatagaaa
      121 tcggaggggg agatgtcaat gaggatggcc gtgcgctcaa accgttcgcc accgatcatc
      181 cccggcgccg acatgcgcat ggcgtggaat atcgagcctt ccgcccagag cgtggtctcg
      241 cctgcctgcc caaagaccga attggcgcca tcgaccacga ccgtgttgtg ggccgcgctc
      301 atggtgtacc aacgcgcacg gggagattcc catccgccga actgaaccgg gggatatccg
      361 agttccggga gcagatcaag tcctttggca aagaggccca ggttcatgcc gtctgtgtgg
      421 ccatggccgc cgcccgaatc gtaatccagc cacaaaacgc ggcgatcatc gcctgctccg
      481 gagcgcatga gagccagatg ccattgctcg aagttgacgc tgcccagatc gagttcctcg
      541 ccgtgctcct gtattacggc ttcgatatca ttcaccacag gcgcgggatc atcgatgtaa
      601 aagtcgtagg gaagacccga aacgccgttg ccattttcgc ggcaggcgat ctgcgcgtac
      661 agcggatcgc gggtctcttt gtaaaattcc cagcagagcg tgaacattga cggcggaatg
      721 agcgtccagc ccgagcctcc cgagccgttg ccgtaacacg tgaaattcag gcctacgtag
      781 cgattaattg gtatgcagta ggcgcccgtg tctccgctct gaggataata acggtccagg
      841 cacagggtat gtgcaaagaa caggaacgtc tttcgaagac ttgggtgccg ttccaacagg
      901 acatgcagaa aggagggatc ggacttcgaa aactctccaa tgaagctgcc cagcgcggag
      961 atggtgtaac tggaataacc cgcgaggcct ttttcaccgg taacgccgtc gaccgcggtg
     1021 gccttggtca acatgggatc gacgatttcc cagaacgccg cttcattctc gggcattccg
     1081 agcactgcat ggatgatggc cagggcaatc tctgtgcggg gatagttggt cgtgatcctc
     1141 gatcggttgg taatggcgtc gcgcaaaata cgggtttcga tattgcgttg aatgtcggcg
     1201 atagtggtct tcgggttgtc gagattgtat tgcactgctt tttttgagag aaacgccgtc
     1261 agttcgggat cgtcctggat ggcttcaaag atcatgtcat aggccatgac gatttcacgc
     1321 gtctcttcgc aggcgtcatg ccaggttgag acgtaccccc gatccgcttt ccgttcatag
     1381 acaaatccct gctcgccgaa gtcgaaggag gggtagagat ccgccacccg gtctatcagg
     1441 atggctgctt tgcgggcgta gaccggatcg ccggtcatca ggtgcgcggc ggccaatgtg
     1501 cgaatcccat ccacgacacc ctgtttccac tgaccgtata ccaagtacgc cgcaataaaa
     1561 cgccagcgct gctcgccttc aacgtaacct gtcccatcat ctaccccaaa aagatggagc
     1621 gggtcgtctg catccggatg tgcagcgttg aagagaagtg agcggtccgc ccgggagggg
     1681 tcaaacacgt ggtgctcgtc gaggcctgag gcataaaaag cctcgaagtc gttcgtggga
     1741 aaaaattcac tgcaatgcgg acattgaacc ttccatgagt gagccaaagc ggccatcttc
     1801 cagctgtaca tgggcacgtt cgctttgcag gccgggcagt atccattcga ccacaccatc
     1861 caggagcggg ggagggtcgc gccaaacatc agttcccaca aatcgtcatc cggcatgttc
     1921 agccaggctt gagcgcttgt aacgattctg tcacgcgcgg cggcagccca gtcaaaacgc
     1981 tccgtactcc ggcgcaccgc cgcgacaatt tcggagctgt agagggtgct tctggttttc
     2041 tgaggttcct gcgcaagccc atcgccgcac aggaaaagaa ccaccaggac tacagctgcc
     2101 ggatagagac gaactcctcg cgacattgga ttccccctca tgtgttttag agtcacaagc
     2161 aagaggcctt cgaatcttca tatcctacca cagcctggcc ctggccgaat ggcagtccac
     2221 ccccggccac gcgttccgcg tgcccacccc cgtcagggga cacatgacgc ggaaccggcg
     2281 atgcctgccc tgtatttggg tcagcgcggg tcgatgggat gggcggccac agggggccgc
     2341 ccctacaatc cggattgcga acgcctgggg ctcccgtggt caacctcgac tttcggagca
     2401 cgtaggggca ggctcctgtg cctgcccttc ttcaagcgca cgcgcatgca ttgacacctt
     2461 ttgcgccccc ttggcatagt agcgccctgc agcacgctga acgatggggt gaattgtgaa
     2521 cgtcgacatc gtaatcgtaa atttcaatac ctgcgaactc ctccgcgaat gcctggtctc
     2581 gatctctgag gcacgcgaag aagtcgattc gatgcgcacg atcgttgtcg ataatgcgag
     2641 caccgacgcc tcggtctcga cgattcgctc cgaatttccc tgggttgagc tggtgcccct
     2701 gccggaaaat ctgggctttg gggcgggcaa taatcgcggc gtcgcccgag gacaaaacga
     2761 gtttatcctc ttcctgaact ccgatgccaa attgacgccc caggccctct ccaggctggc
     2821 ttccagactt caggcctctc ctctcgtagt agcagttggc ccccggctga tctatccgga
     2881 tggctcgttt cagccctcct gccgccgctt tcccacggtg tggcggtggc tctggtctac
     2941 cacaggcctg gccagtcgat tcccccggac cttcaagtcg ttccgtcact ggctcgatga
     3001 ggaagagcac gttgacgggc aagccgtcga catggtctcc ggtgcctgtt ttctcatgcg
     3061 gcgcgactat tttgagagta ttggcggatt cgacgagaac ctgtttttgt acgaggaaga
     3121 attcgacgta ttctatccgg ccgcgcggcg agaacttgag gtcgggtact gcggcgaagc
     3181 gacggttatc cacggagcgg gtaaatccgt tgaggcgaac cgcatggccg catttgcccg
     3241 gtttcacctt gttcgaagca aatattacgc cttccgaaag cattatgggc gcctggcagc
     3301 gcgtctcgcc tactgggtgg atctggccgt tttcggaacg accggcacgt tgcgcaagat
     3361 tcgtggaaag acggcaggcg cgctgctgac ggttcgcatc tgccggctgg cgtggcccgc
     3421 ctcgcgcctg acgggaacgg aagtgcgttc gctcaagtgg gatctgcccg ggtaacgcct
     3481 ccggcaaccc tcagcgctgc accagcatcg gggcggggac actggcaaac tgccggttgc
     3541 gcgcgcgctg cacgagcctc gacaactcca gaatgggcac gagcgcgccc agcaatcggc
     3601 aatggtcttt tccatcaagg tgttcttgcc acatccgacg ccggaactct ggattgagta
     3661 tgtcgtcggg aagagggtat tcgccgcgga tcagactgtc catccagtcg cgcagttcac
     3721 cgcgaagccg ctctgcgaac agcgccggag cctggcgatg ggtaggtccg tgccagccag
     3781 cccaggcctc gaccttcgca ctggcacggc gcatgacctt tgaggccagg atccgccatt
     3841 cgggggcaag tggaggcaga agggttgttt gccagcgaat ggccgccaca tcgggaaaca
     3901 cagtccgaag catcgcccgg tacgtacggt gccgggcgcg caattccgcg ggaattcggc
     3961 gaaccaggtc gttgaagtcg taatcgtaga agcaggcggc cgattcggca gcctggcgca
     4021 tcagtacggt gcccatagcc gttttgcggc ggacgtgatt atcaaacaga aagcgatggg
     4081 cagcgtcgcg ggttcccagg tggcgcagtt cctcaaactg ctgcttgaaa acggagagcg
     4141 gaaaatcttc ctggcgaaaa tcgcgcacga ccggcatagc ttccggcagc tcggcctcct
     4201 ttaaaaaccg gttataccga tggaagaggt tttcggccaa ttcggcggga cgcatcgggg
     4261 atgcccattg cggacgcaga aaggtctccc caaggaccgc atctcccgcg aagccgttta
     4321 aaaccacatc caccccggca atgatgtgca gatgggccag aaaatgcatg tcgaatgcgt
     4381 tgcacatgcc ttcggttatg gccacccctt cctcgaaccg ttccgccaga gagtcatatt
     4441 cataggggca gtcctgatgc tcgatcccca gggcgcgtgc gatttcggag gcgtagaggc
     4501 gatcgtaaca cccgtcttca ccccaggtga aggttcgggg cgatatccct gtctgaaccg
     4561 cggctgcggc gacacaccgg gaatccagac ccccgctgag cgtcacgccg atgcttcggc
     4621 ctgctcccgc ctgacggcgc acggcctcaa ccaaagccgc gcccgcccgc cggccccact
     4681 cctcggcaga aaggccctgt tccgcggggt aatcccagac ccagtacgtc tgccagtcga
     4741 tgcggtccgg ggttttcgtc atcatcgtgc ccggtggaaa ggccgaaacg tccttcacaa
     4801 gcgtgcgccc gcccacaaaa tcgtccacag tgagtatctg cgctaccgcc agagtgtcaa
     4861 tgccgccggg aaccaccccg gcgaggacgg ggcccagggt gcggctggac aggcaatacc
     4921 gcccatcctt ctgagcccag tacatcgggt acagaccgta ccgatcgttg gctgcgatta
     4981 acgtgcgggc ttcgggatcc cacagcatga agaaaaaggc gccgttcagc gcgggcagcg
     5041 tagcgagcag ctgcttgtcc cgggcgagcg acgcgagcag agccaggtcg gtttccgcgt
     5101 ccggccgcgc gtgccggcac cggtcaagcg ccgcacaggg gtgaagttcg ccgaccagaa
     5161 gcccccagaa cccagattct tcgaggtagc tcggcatggc cccttgaccg aatttatccg
     5221 gactcaccag gccgaaaatg gcgccgttcg attcggcata atggttgacg cgctcatgcc
     5281 cgctggccag cagagcgctg aacgaatgcg ccagtgtgcg catcgcgcca gggtcgggca
     5341 gttggcaaaa atacgcgtta ctcatggtcc agtgaccttt cggcggtcac gtcgcaagac
     5401 ccagcgcagt tcgccccagg caatgctgag tttcgagaaa taggaatgca ctgcatacat
     5461 cagcgaaacg ccggccgcat ggcctttcat tcgggtctgc gcaaattttc ttgcaagaac
     5521 acaccctgcg acagccaaaa tcgacacgcg aaggcccgta gttccgataa tcagcccggc
     5581 aagcgcgcca aacattagca gggcccagcc tgcgtttgta aggcattctc ttcgccacaa
     5641 gggatcgggc gtccgccagc agattgccgc gatctcggca taggtctgtc cgcaacgcac
     5701 attccggcgc caataatcac gcaaaccccg gaaatccagg ttatgccggg ccatggtctg
     5761 gtttatctgg taaatcttcc gcccaaggat gttccgcagg cgccaacaca acagcggttc
     5821 ttcaccatac gccacctccg tgggaaacgc acccgcttcg cgcagcagtc cagcaacata
     5881 catggcagcg cc
//
LOCUS       DAOPBQ010000193         5877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11825_length_5877_cov_4.168155, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000193 DAOPBQ010000000
VERSION     DAOPBQ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11825_length_5877_cov_4.168155"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKW60_07530"
     CDS             <1..204
                     /locus_tag="PKW60_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPJ99324.1"
                     /translation="YLISSADMIQEAWLNGVESILLTSGASAPERLVQGVIEYFKTRG
                     PCDVVESEAELEGVHFKLPAAIA"
     gene            455..1792
                     /gene="ffh"
                     /locus_tag="PKW60_07535"
     CDS             455..1792
                     /gene="ffh"
                     /locus_tag="PKW60_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPJ99325.1"
                     /translation="MFESLTKRLEAAFKNIRGQGKLTEKNMKDSLQEIRRALLEADVN
                     YKVVKQFIADIQEQALGQKVLDSIQPGQQIVKIVHDALVNTMGGANEPLRLADTPPTL
                     ILMVGLQGQGKTTSAGKIALHLKKKGHKPLLVGADVYRPAAIKQLEIVAQQAGVECFS
                     LGEHADPVDTCVMARGEAQMRQCDVIIMDTAGRLHVDEQMMGEVRRIQAQVAPHEILF
                     VANAMTGQDAVNSAKQFHDALPLTGVVLTQLDGDARGGAAISLINVTGCPIKFVGTGE
                     KLDALEPFHPKRMADQILGMGDIVSLVEKAQEVVDREQALQFQEKARKATWDLEDFLQ
                     QMQQLKKMGSLGDLIQKIPGMKKMMGQGMMDVDEDEMKYVEAIIYSMTPEERQKPKII
                     NGSRRRRIADGSGTSVQEVNRLLKDFEQMRKMMKNMMKGGKKGRRGMPMLPGF"
     gene            1907..2158
                     /gene="rpsP"
                     /locus_tag="PKW60_07540"
                     /pseudo
     CDS             1907..2158
                     /gene="rpsP"
                     /locus_tag="PKW60_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012094939.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            2327..2557
                     /locus_tag="PKW60_07545"
     CDS             2327..2557
                     /locus_tag="PKW60_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPJ99326.1"
                     /translation="MKQLIEFVAKKLVDHPEDVQVTVIESEDGQCYELRVHPDDMGKV
                     IGRDGRTAKAIRTILSTAANKQDMRATLQIVE"
     gene            2576..3103
                     /locus_tag="PKW60_07550"
     CDS             2576..3103
                     /locus_tag="PKW60_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99327.1"
                     /translation="MTTRTVVAGKVHSVNPGKRQVRIQAVDGGVPDDAFSGRLHLRLP
                     DGREIRPLVSALRRPEGWVVVEFTPGTPRDSVAGMRGAEVLWEADEDALAFQGRFAAD
                     ALPGMRLLGPGGVELGEVVGVLESPAHAVLEIQGGDGRRLLLPMVREVVERIDWESRA
                     IIVGDIAPFVVDDAH"
     gene            3093..3845
                     /gene="trmD"
                     /locus_tag="PKW60_07555"
     CDS             3093..3845
                     /gene="trmD"
                     /locus_tag="PKW60_07555"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPJ99328.1"
                     /translation="MRIDVLTLFPEFFDAPLRASLLGKALELGTFEVAITNIRDFATD
                     RHRTVDDAPYGGGPGMVMRCEPIAAALESLAGRNSLQRVVLMSPRGRRLDQNLVRELA
                     RVPDLVLICARYEGVDERVSTALCTEEISIGDYVLSGGELPALVLIEALSRMLPGVVG
                     DWESVTTDSFYEGLLGPPQYTRPAMFRGLEVPAVLREGHHAAIQRWRRREALRATRTR
                     RPDLLVNCSEEDRLLLAEIDREEAERKRGEQP"
     gene            3842..4228
                     /gene="rplS"
                     /locus_tag="PKW60_07560"
     CDS             3842..4228
                     /gene="rplS"
                     /locus_tag="PKW60_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPJ99329.1"
                     /translation="MKFTKAVEALSQEQKKAAEKVPQFRVGDTVRVHFRIVEGEKERI
                     QVFEGVVIGRSGGEGANAKFTVRRVAYGEGVERVFPLHSPRVEKVDVVREGKVCRAKL
                     YYLRDRAGKAARVKAKGRSTAVKSEQ"
     gene            4371..5174
                     /locus_tag="PKW60_07565"
     CDS             4371..5174
                     /locus_tag="PKW60_07565"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPJ99330.1"
                     /translation="MPKRTLNEIRAAAQAGTPHAKRPAKDPADAARSGISRRQTARPE
                     ATQGREELQAGLLERQAAEQGFERVAGVDEAGRGPLAGPLVAAAVVLGHPLPELNDSK
                     QLTPATRARLFAMLQNGPHAIGIAIIPPEEIDRLGIQPANYAAMRRAVNQITPPPDYL
                     LVDGFDVPGCPLPQCRVIKGDCRSQSIAAASILAKVTRDRIMEELDGLYPQYGFARHK
                     GYGTREHLEAIARFGPCPHHRRSFAPLARSLETEPLFSKLDKDASSCAT"
     gene            5162..>5877
                     /locus_tag="PKW60_07570"
     CDS             5162..>5877
                     /locus_tag="PKW60_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ99331.1"
                     /translation="MRHVTIALCLMAILGCETLPYAPAEPPSARAQAFNHYLMGDIHR
                     RNGRFEQANSELAAALKYVPDDPNLMKHIIRGYLLVHDLENALVYAKNLVSVYPGDPA
                     AWFILGRIYRQQERYDEATEAFKKALTLTSFNVFIYDELLQSEEESNDLVSVVDIYLK
                     LVQLHPDSALLYERLGLSLARIRKNEEAKVYLQKALELDSGLTSARYLLGLIHLELGE
                     TDSAAAQLQRYLAMSPDDVSA"
BASE COUNT         1183 a         1730 c         1959 g         1005 t
ORIGIN      
        1 tacctcattt catccgccga catgatccag gaggcgtggc tgaacggtgt cgaatcgatc
       61 ctgctcacct ccggcgcgtc tgcgcctgaa cgattggttc agggtgtgat cgagtacttc
      121 aagacgcgcg ggccctgcga cgtggtggag agcgaggccg agctggaggg cgtgcacttc
      181 aagctgcctg ccgccatcgc gtaagcgccg cactcccgct cgcgggaatg cctccaggca
      241 gaggtctcac ccctgctccg gcgcaaaaac gccgcagggt gccgtgagcc gcgttcaggc
      301 gcggatcaat tgcggcaaat cgaatagaat catcaggttg gggcggggcc atcgagattg
      361 cattgctgct gtttcgtatg gtacgataca cgctcgattt caggtgagat ctgtctattt
      421 tgctgggaag ctctgtctct aacgcgagta cggcatgttt gaatcgttga caaagcggtt
      481 ggaagccgcc ttcaagaaca ttcgcggtca gggaaaactg accgagaaga acatgaagga
      541 cagcctccag gagatccggc gggcgctgct cgaggccgac gtcaactaca aggtcgtcaa
      601 gcagtttatc gcggatatcc aggagcaggc gctgggccag aaggttctgg acagcatcca
      661 gccgggtcag cagatcgtca agatcgtgca cgacgcactc gtaaacacca tgggcggggc
      721 gaatgagccc ctgcgcctgg cggacacccc gccgacgctg atcctgatgg tggggctgca
      781 gggccagggc aaaacgacct cggcgggcaa gatcgcgctg catctcaaga agaaggggca
      841 taagccgctc ctggtcggag cggacgtgta ccgcccggcg gccatcaaac aactggagat
      901 cgttgcacag caggcgggcg tggagtgttt cagcctcggc gagcacgccg atcccgtgga
      961 cacctgcgtc atggcacgcg gcgaagccca gatgcgccag tgcgatgtca tcatcatgga
     1021 cacggcgggc cggctgcacg tggacgagca gatgatgggc gaggtgcggc gcattcaggc
     1081 ccaggtagcg ccccacgaaa tcctgtttgt tgccaatgcg atgacgggtc aggacgcggt
     1141 caacagcgca aagcaatttc atgacgcgct gccgctgaca ggtgtcgtgc tgactcagtt
     1201 ggatggcgat gcgcgcggcg gcgcggcgat cagcctgatc aacgtgacag gctgtccgat
     1261 caagtttgtc gggacgggcg agaagctgga cgctctcgaa ccgttccacc ccaagcgcat
     1321 ggccgaccag atcctgggga tgggcgacat tgtgtcgctt gtggaaaagg cccaggaagt
     1381 cgtcgatcgg gaacaggcgc tccagtttca ggaaaaagcg cgcaaggcca cctgggacct
     1441 cgaagacttt ctgcagcaga tgcagcagtt gaaaaaaatg ggctcgctcg gcgatttaat
     1501 ccagaaaatc cccgggatga agaaaatgat gggccagggg atgatggacg tcgacgaaga
     1561 cgagatgaaa tacgtggagg ccatcatcta ctcgatgacg ccggaagaac ggcagaagcc
     1621 caagatcatc aatggcagcc ggcgccggcg catcgcagac ggaagcggta ccagcgtgca
     1681 ggaagtcaac cggttgctca aggacttcga gcagatgcgg aagatgatga agaacatgat
     1741 gaaaggcggg aagaaaggcc ggcggggcat gcccatgctg cccggcttct gaggccgcgg
     1801 ccggtgtggc cgcattttct gccgcatggc attttgagag tcgcagcata ggcacgacgg
     1861 attcaggaag accgcggaat cgccgccgtc aggagagaaa ggaaccatgg caacagtaat
     1921 tcgtatgaag cgaggcggac gcacccacgc gccgtattac cgggtggtgg tgatggatgc
     1981 gcggagccgc accaaaggcc gcgtaatcga agaaatcggc gtatatcatc cgtgcgcccg
     2041 gcccgaaccg cgtgccgaaa tcgatgccag acgcgcgctc caatggctat gcaacggcgc
     2101 gcagccttcc gacaccgtgc ggtctcttct ggcccggcag ggcgtcatgg agatgttcgc
     2161 caagggaacg aaaccggaag agctgccggg agcctctccg gccgaagccg tcgcggccga
     2221 agccgtcgcg gccgaagccg aggcaacggc gccggacgcg taaggcccgg ccgcccgggc
     2281 cggctctgcc ccgcgtgggc acgggccgtg aacgaggatt cgcaccgtga aacaactcat
     2341 tgaatttgtt gcgaagaaac tggtcgacca ccctgaagat gttcaggtta cggtcatcga
     2401 aagcgaagat ggccagtgct atgaattgcg cgtccatccg gacgatatgg gcaaggtgat
     2461 tggacgagac gggcggacgg cgaaggccat tcgcaccatt ctcagtacgg ccgctaacaa
     2521 gcaggacatg cgggccacgc tccagatcgt ggagtgaggc gcgctggaat gccgaatgac
     2581 cacgcgcact gtagtggcgg gcaaggttca ctccgtgaat ccgggcaaac ggcaggtgcg
     2641 catccaggcg gtggacggcg gggttcctga tgacgcgttt tcaggccgct tgcaccttcg
     2701 gctgccggac ggccgggaaa tccgccccct ggtaagtgca ttgcggcgcc cggaaggatg
     2761 ggtcgtcgtg gaattcacgc cgggaacgcc gcgcgacagc gtggccggca tgcgcggcgc
     2821 ggaggtgctc tgggaggcgg acgaggacgc gctcgctttc cagggccgct ttgcggcgga
     2881 cgccctgccg ggtatgcggc tgctcgggcc cggcggggtc gaactgggcg aagtcgtggg
     2941 tgtgctggag tcgccggccc acgccgtgct cgaaatacaa ggcggcgatg ggcgaagact
     3001 gctgcttccg atggttcggg aagtggtaga acggatagac tgggaatcca gggcgatcat
     3061 cgtgggagat attgccccgt tcgtggtgga cgatgcgcat tgacgtcctc accctgttcc
     3121 cggagttttt tgatgccccg ctcagggcga gcctgctggg caaggccctg gagctgggta
     3181 cgtttgaggt cgcgataacc aacattcggg atttcgcgac cgaccggcat cgtacggtgg
     3241 acgatgcgcc gtatggcggc ggtccgggca tggtcatgcg gtgcgagccg attgcggcgg
     3301 ctctggaaag cctggctggc cgcaactcgc ttcagcgtgt ggtgttgatg tcgccgcgcg
     3361 gccggcgcct tgaccagaac ctggtccgcg agctggcccg tgtgccggac ctggttttga
     3421 tttgtgcgcg gtatgaaggc gtggatgagc gcgtgagcac cgcgctttgc acggaagaga
     3481 tttcgattgg cgattacgtg ctgagcggag gtgaattgcc tgcgctggtc ctgattgaag
     3541 ccctgagccg gatgttgccc ggtgtggtag gcgattggga atcggtcacg acggactcgt
     3601 tttacgaggg actgcttggg ccgccccagt acacgcgccc cgcgatgttt cgcggcctcg
     3661 aggtgcccgc ggtcctgcgc gaaggccatc acgccgcgat tcaacgatgg cggcgcaggg
     3721 aagcgctgag ggcaacgcgc acgcgccggc cggatttgtt ggtgaactgt agcgaagaag
     3781 accgtttgct cctggcggag atcgaccggg aagaagcaga acggaagcga ggagaacagc
     3841 cgtgaaattc acgaaggcag tggaagcgct cagccaggag caaaaaaagg ccgcggagaa
     3901 ggtgccgcag ttcagggttg gcgacaccgt ccgtgttcac ttccgcattg tggaaggtga
     3961 aaaagaacgc attcaggtgt ttgaaggagt ggtcatcggc cgctcaggcg gcgagggcgc
     4021 caacgccaag ttcaccgtgc gccgcgtggc ctacggggaa ggcgtcgaac gcgtattccc
     4081 gcttcattcg ccgcgcgtgg aaaaagtcga cgtagtccgc gagggcaagg tgtgccgcgc
     4141 caagctgtac tacctgcgcg accgcgccgg caaagcggca cgcgtgaaag ccaaaggccg
     4201 ttccaccgca gtcaaaagcg aacaataacc gcgacgtttc gcgctcacga agttcccggt
     4261 gttctgacgc ccgcagcggc ccgccttgcg gtgggctcgc atgcgggcta ttctacaggc
     4321 gttgcgccat cgggcgggat gctcgggcaa actggtctgg gatggaatcg atgccgaaac
     4381 gcaccctgaa cgaaatacgc gccgctgcac aagcgggaac gccgcatgcc aagaggcccg
     4441 caaaggaccc cgcagacgcg gcccgctccg gcatctcacg acgccagacg gcccgccccg
     4501 aggcaacgca ggggcgtgag gaactccagg ccggcctcct ggagcgccag gcggcagagc
     4561 aggggtttga gcgcgtggcc ggggtcgatg aagcgggccg cggccctctg gcgggcccgc
     4621 tggttgcggc ggccgtcgtg ctgggccatc ccctgcctga actgaatgat tccaagcaat
     4681 tgacgccggc cacacgggcg cgcctcttcg ctatgctgca gaacggcccc cacgccatcg
     4741 gcatcgccat aatcccgccc gaggaaatcg accgcctcgg cattcaaccg gccaattacg
     4801 ctgccatgcg ccgtgctgtg aatcagataa cgcccccgcc cgactacctt ctggtggacg
     4861 gttttgacgt gcccggctgc ccgctccccc agtgccgggt gatcaagggg gattgccgtt
     4921 cccaatcgat cgccgccgcg agcatcctgg cgaaagtcac gcgagaccgc attatggaag
     4981 agctggacgg cctgtatcct cagtacgggt tcgcgcgcca caagggatac ggcacacgcg
     5041 agcacctcga ggccattgcg cggttcgggc cgtgcccgca tcaccggcgc tcgttcgccc
     5101 cgctggcccg ttccctggaa acggagccgc tgttttcgaa actcgacaag gacgcttctt
     5161 catgcgccac gtgactattg cgctctgcct gatggccatt ctgggatgcg agaccctgcc
     5221 ttacgccccg gccgagcccc ccagtgcccg cgcacaggcg ttcaatcatt acctgatggg
     5281 cgacatccac cggcgcaacg gccgtttcga gcaggcgaac agcgaactcg cagcggcgct
     5341 gaaatacgtc cccgatgacc cgaacctcat gaaacacatc atccggggct atctgctggt
     5401 tcatgatctg gaaaacgccc tggtgtatgc gaagaacctg gtgtccgtct accccggcga
     5461 tccggccgcc tggttcatac tgggccggat ctaccgccag caggagcggt acgatgaggc
     5521 gacggaagca ttcaaaaagg cccttactct gacttccttc aatgtcttca tctacgacga
     5581 actgcttcaa tccgaggaag agtccaacga tttggtgtcc gtggtggaca tttacctgaa
     5641 gctcgtacag cttcatcccg attccgcgct gctctacgaa cggcttgggc tgagcctggc
     5701 gcgcatccgg aaaaatgaag aggcgaaggt ctatttgcag aaggccctcg agctggactc
     5761 cggattgacc agcgcccgct atttgctcgg cctgatccat ctggaactgg gcgagactga
     5821 ctctgcggca gcccagctgc agcgctacct ggccatgtct cccgacgacg tgagcgc
//
LOCUS       DAOPBQ010000194         5865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11852_length_5865_cov_3.172289, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000194 DAOPBQ010000000
VERSION     DAOPBQ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11852_length_5865_cov_3.172289"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..757
                     /locus_tag="PKW60_07575"
     CDS             263..757
                     /locus_tag="PKW60_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ99332.1"
                     /translation="MNRRTAVNVTLALLLVLSTGFVAPCRAQEGGSSHNEKGLGYFKK
                     GFYEYAPHNKHLEANQYYEMAIEEFMKALAENEGDAAAHRNLARVYQAQKKYALSAKE
                     YRRVTELRPGDIDAYVNLAVAYSHLNRYEEAIEQLERAKTKTDDPGAIKRLNGYIEKL
                     EQAR"
     gene            754..2190
                     /locus_tag="PKW60_07580"
     CDS             754..2190
                     /locus_tag="PKW60_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ99333.1"
                     /translation="MTGTRHRDATQGSACLMQAFRRALPMAALAVLLLSAAASPTAAG
                     NPHSAFYSPANDRVFWFIHASDIHLGARGSKDSENLQWLVTTAQNTICPSFIVVSGDL
                     TDSTNGNILGYPNGPHQEEWNEYKSIVDPNVTADTYYDIPGNHDAYSDQYFAYYLANS
                     VQGRATGGTQHSWTRNFWFGEYHFLGVNTADNTGAAFSFLPPYYGDHAGLDSTELAFI
                     SSELQAHPNAALTLVFGHHPLAATGDPKDTYIYYGRDEFVGLMNSNGASLYGYGHTHD
                     SSESFYTAGMSEGVFYFNVSALGKDSPNQYTVTAIDCNGIASVTQNVNTWPVVLVTAP
                     MDRYLGGNVNPYTYSIPNSPANPIRALVFDTAAVNEVQYRIDLSGSWRAMQRTAAGNP
                     AYPHLWEAVWNASGLAPGEHTVEVRATTASGVRTDTVRVNVQGTLAGDFPPPDCDVDG
                     SDLAVLIGNPSLMDISTFAQNYGKNTCP"
     gene            complement(2245..3297)
                     /locus_tag="PKW60_07585"
     CDS             complement(2245..3297)
                     /locus_tag="PKW60_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPJ99334.1"
                     /translation="MSDNFIWTCLSHEYHGLGSHVKAQTGSDLHIDICAAFRYFPVMA
                     RKPRIEIEGALYHVMTRGNQRQRIFRSRKDYARYLEFLCRYKERYGFLLFAYVLMPNH
                     VHLLVEMSHVPLSKVLQGINQSYTMYFNRRYETVGHLFQGRYKAILCDRDEYLLALVK
                     YIHLNPVRAGAARTADDYPWSSHPAYAKKATDSRLVDADFVLRLFSENRAAASRRYQE
                     FIDGGPAVEKAEIYRTADQRVLGDEEFVADVKSRVPFEMNGRGRQRAYSLEDIAAAIE
                     RVTAVSIDRMRTQTKSRTVADGRRLFCRAAKEFSCRNHEIAAFLRKDPAAVTRYLKEY
                     TGIEADVKKVLASLEG"
     gene            3420..4802
                     /locus_tag="PKW60_07590"
     CDS             3420..4802
                     /locus_tag="PKW60_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPJ99335.1"
                     /translation="MRDESNHKSPPGRGQPAEKGWFRTLVERSPMGISVARDGITLYA
                     NPSCARLFGYDHPGELVGTSQLGRVAPESRRQVTDCIERRRRGEPAPLGYEIVGLRKD
                     GTTFPLCVEVNLIDSGGCPLSMAYFTDLTERKRAESALLDSGKRFRNLVESIDEFLAD
                     NDTNGICRYASPRVFDLLGYQPHEVVGRTPFDLMPNDEANRLREIFRRASGLGEPLHS
                     FEYAMLHKSGRLVFLETSATPYFDGAGRLEGYRSITRDVTARREAAEALRRAREELEA
                     RVRERTEELASLNEKLLKDIEERQRIEETLRQRELELKQRNIKLEEMNVTLNTILEQR
                     DRDRKILEQRVSANINELLKPLLEKLAQCRTGTQRQSCLEIIESSLDEVVSPFSQQLT
                     DHSFRLTPTEIQIANCICKGMRSKEIASLLRLSKGTIDFHRNNIRRKLGLNRRKTSLR
                     SHLLSLSMKI"
     gene            5345..>5865
                     /locus_tag="PKW60_07595"
     CDS             5345..>5865
                     /locus_tag="PKW60_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99336.1"
                     /translation="MRIKRKSPCFILAAMVLLLLPTAALAADVDVFAEGAYNAQYLDV
                     YIYANINTGNLCSYGVKLTYDKTRLNTPTATKNVAVWYFGSSSPGNTYMDPVVDSAAS
                     PATITFIGGKLDTGNPTAGVTGMRVLLGSVRFVRTEGSLGLGPTAETYFGITASLGRR
                     SPYDNFVTTGGQV"
BASE COUNT         1285 a         1851 c         1647 g         1082 t
ORIGIN      
        1 gattattctg cgaatcagcc cttgccggat cgccgtcagg ccgctatgat gaatgcagat
       61 gaatccccgc tgcccggccg ataggcctca tgaggtcccg gctcgagcct gcacgctcag
      121 cccttcatga gatggcatcg cgccgtgcag ccggccgggt ttcgggaagc gcacggctcc
      181 cgagcggcaa cccgttcacc cctgcaacaa ccgatcttcc agatcccatc tccggtatga
      241 gaaacgcgcg agggataagg ccatgaaccg aagaacggcc gtcaacgtga ccctggccct
      301 gcttcttgtc ctttcaacgg gcttcgttgc gccgtgccgg gcccaggagg gcggctcgtc
      361 acacaacgaa aaaggcctcg gttatttcaa gaagggcttc tacgagtacg caccccacaa
      421 caagcacctg gaggcaaacc agtactacga gatggccatc gaggaattca tgaaggccct
      481 ggcggagaac gagggcgacg cggccgcgca ccggaacctc gcgcgagtct atcaagccca
      541 gaagaagtac gcgctttccg cgaaggaata caggagggtc accgagttga gacccgggga
      601 catcgacgcc tatgtcaatc ttgccgttgc ctattcccat ctgaaccggt acgaagaggc
      661 catcgagcag ctcgagcggg ccaagacaaa gaccgacgac cccggggcga tcaagaggct
      721 gaacggatac atcgagaagc tggagcaggc acgatgacgg ggacgcgaca ccgcgacgcg
      781 acgcagggct cggcctgcct gatgcaggcc ttcaggcgcg ctttacccat ggccgccctg
      841 gccgtcctcc tgctgtcggc ggccgcttcc ccgaccgctg cgggcaaccc ccactccgcc
      901 ttctacagcc cagcgaacga cagggtcttc tggttcatcc atgcgtcgga catccatctc
      961 ggcgcccggg gcagcaagga cagcgaaaac ctccagtggc tcgtcacgac ggcgcagaac
     1021 acgatctgtc cgagtttcat cgtcgtctcc ggggatctga ccgattcgac aaacgggaac
     1081 atcctggggt atcccaacgg tccgcaccag gaagaatgga acgaatacaa gagcatcgtg
     1141 gaccccaacg tcaccgcgga cacctattac gacattccag gcaaccacga cgcgtacagc
     1201 gatcagtact tcgcctatta cctggccaat tcggtccagg gccgggcaac aggggggacg
     1261 cagcactcct ggacccgaaa tttctggttc ggggagtacc attttctggg ggtcaacacg
     1321 gcggacaaca cgggggccgc gttcagtttc ctgccgccct attatggaga tcatgccgga
     1381 ctcgactcca cggaacttgc cttcatctcg tcggagttgc aggcacaccc gaacgccgcg
     1441 ctgaccctgg tcttcggcca ccaccccctg gccgccaccg gcgaccccaa ggatacgtac
     1501 atctactacg gcagggacga gttcgtcggt ctgatgaaca gcaacggggc gtccctctac
     1561 ggatatggcc acacgcacga ctccagcgag agcttttaca cggcggggat gtcggagggg
     1621 gtgttctatt tcaacgtttc ggcgctgggg aaggacagcc cgaaccaata cacggtgacc
     1681 gccatcgact gcaacggcat cgccagcgtc acacagaacg tcaacacatg gcccgtcgtt
     1741 ctcgtcaccg cccccatgga ccgctatctg ggcgggaatg tcaatcccta cacgtattcc
     1801 attccgaatt ccccagccaa cccgatccgc gccctcgtct tcgacaccgc cgccgtgaac
     1861 gaggtgcagt accgcatcga cctgtcgggg tcgtggcgtg ccatgcagag gaccgccgca
     1921 ggcaatcccg cctacccgca tctctgggag gccgtgtgga atgcctccgg ccttgccccg
     1981 ggtgagcaca ccgttgaagt ccgtgcaacc acggcatcgg gcgtgcggac ggacacggtc
     2041 cgggtgaacg tgcagggcac cctcgccggc gatttcccac cgccagactg cgacgtggac
     2101 ggaagcgacc ttgcggtgct gatcggcaac ccctccctga tggacatctc gacgttcgcc
     2161 cagaactacg gcaagaacac ctgcccgtag cctcaccaaa tcaaagggga ggggtcagac
     2221 ttgcatattt gatatttaag ccattcatcc ttccagcgaa gcgaggactt tcttcacgtc
     2281 ggcctcgatc cccgtgtact ctttcagata ccgggtcacc gcagcgggat ccttcctcag
     2341 aaaagcggcg atttcatggt tccggcagga aaattctttc gcggcccgac agaacagcct
     2401 ccggccgtca gcgaccgttc ttgatttcgt ctgagtgcgc atccggtcaa tcgagaccgc
     2461 tgtaacgcgc tcgatcgccg cggcaatgtc ctccagggaa tacgcgcgct gccttccacg
     2521 cccgttcatc tcaaaaggaa ccctgctttt cacatccgcc acaaactcct cgtccccgag
     2581 cacacgctga tccgccgtac ggtatatttc cgccttttca acggccggcc ccccgtcgat
     2641 gaactcctga taacgcctgc tcgcggcggc tctattctcc gaaaacaacc ggagaacgaa
     2701 gtccgcgtca acgagacggc tgtctgtcgc ctttttcgca tatgcgggat ggctgctcca
     2761 ggggtaatcg tcggcggttc gcgccgcacc ggccctgacc ggattgagat ggatgtattt
     2821 caccagcgcc aggaggtatt catccctgtc gcagagaatc gccttgtagc gaccctggaa
     2881 caagtgtccg accgtttcat acctgcggtt gaaatacatc gtgtagctct ggttaatccc
     2941 ctggaggacc ttcgagaggg ggacgtggga catttcaacg agaagatgga cgtggttggg
     3001 catcagtaca taggcgaaaa gcaggaagcc gtaacgctcc ttgtaccggc agagaaactc
     3061 gagataccgg gcatagtctt tcctcgatcg aaagatcctc tgccgctgat ttccccgggt
     3121 catgacgtgg tagagcgccc cttcaatctc gattcttggt tttcttgcca tgaccgggaa
     3181 atagcggaaa gcagcgcaaa tgtcaatatg caagtctgac cccgtttgtg ccttgacatg
     3241 gctgccgagc ccgtgatact cgtgcgacaa acacgtccag atgaagttgt cgctcatcga
     3301 cctgaactcc tccttcccgg agcggcgttg acccatcgag cggcggaccg gcagtaagcc
     3361 agtaggccgc gcgggactgt cagcgcgccc gcgtcatccg cttgaggcca ggaccacacg
     3421 tgagggacga atcgaatcac aaaagccccc ccggccgtgg ccagcccgct gaaaagggct
     3481 ggttcaggac gcttgtcgag cggtccccca tgggcatctc ggttgcccgc gacggaatca
     3541 ccctctatgc aaacccttcc tgcgcacgcc ttttcggtta cgaccacccc ggagagctcg
     3601 tcggcacgtc acagctcggc cgcgtggccc ccgaaagccg ccgtcaggtg acggactgca
     3661 tcgagcgccg caggagaggc gagccggcgc ccctgggcta cgagatcgtg gggctgcgca
     3721 aggacgggac gaccttcccc ctctgcgtcg aggttaacct catcgactcg ggcggctgcc
     3781 ccctgtcgat ggcctatttc acggatctca cggagaggaa gcgcgcggag tcggcgcttc
     3841 tggacagcgg gaagcgtttc cggaatctcg tcgagtccat cgatgaattc ctcgccgaca
     3901 atgataccaa cgggatatgc cggtacgcca gcccgcgcgt cttcgacctg ctcggctatc
     3961 aaccccatga agtcgtcggc aggacgccct ttgacctcat gccgaacgac gaggcgaatc
     4021 gcctcagaga gatattccgt cgggcctcgg gccttggaga gcccctgcat tcgttcgagt
     4081 atgccatgct gcacaaatcg ggcaggctcg tcttcctgga aacctccgcg accccatact
     4141 tcgacggggc aggacggctg gaggggtacc ggtcgatcac ccgcgacgtc accgcgcgaa
     4201 gggaggcggc ggaggccctg cgcagggcga gggaggagct cgaggcaagg gtacgcgaac
     4261 gcacggagga gctggccagc ctcaacgaga agctgctcaa ggacatcgaa gagcgccagc
     4321 gcatcgagga aaccctccga cagagggaac tggagctgaa acagcgtaac atcaagctcg
     4381 aggaaatgaa cgtcacgctg aacaccatcc tcgagcaacg ggaccgggac cggaaaattc
     4441 tcgagcagag agtctccgca aacatcaacg agcttctcaa gccgctcctg gaaaagctcg
     4501 cgcagtgccg gaccggcaca cagcggcagt cgtgcctgga gatcatcgag tcgagtctcg
     4561 atgaggttgt ctcccctttt tcccagcagc tgacggacca ttcattcagg ctcaccccga
     4621 cggaaatcca gatcgccaac tgcatctgca agggcatgcg ttccaaggag atcgcctccc
     4681 tgctgcggct ctcgaaggga accatcgact tccaccgcaa caacatccgc cgcaagcttg
     4741 gcctcaaccg ccgcaaaacc agtctccgaa gtcacctcct ctcgctgtcc atgaaaatat
     4801 aaggatatcc cctatattta accggtatat ttcctatatc atttctgaca ggccgtcgga
     4861 cgacgtgccg tcccgaatcg ccggactgtc ggattctttt acagatctac gcccgccccg
     4921 ttttcgctgt tattacaagg actacagaaa aaaatcgtct cgattaagac ttccgctgtc
     4981 ggcgcattta acaattctgc aatcacagca tggtggtcaa aataaaataa tctcataata
     5041 cccgacgctt aggtagcagg agcagttttt gctcagatac ggtcgaagca gcgtgttttc
     5101 atgcacgccc gaggcgtgta gtcgtgtcgt tttcaaccta tgtaactaat taaaccaatc
     5161 ttcatcgcat tcaccgcggc ctggttgctt ggcctggtgg ccgtccgccg caggataaaa
     5221 aagcaaatcc tcccgccctc ccccttataa aaggggaaca aggggggatt cggtattacg
     5281 taaaacgtaa cacgtaaaac ctaaaactca cgccctgcag gcgtgcagaa ggaggacgaa
     5341 gagcatgaga ataaaacgga aatcaccctg tttcattctg gcagcgatgg ttctcctgct
     5401 gctgccgaca gcggcactgg ctgcggatgt ggatgtgttc gccgagggcg cgtacaacgc
     5461 gcaatacttg gacgtttaca tctacgccaa catcaacact ggtaatctgt gcagttacgg
     5521 tgtgaagctc acgtacgata aaaccagact gaacacacca actgcaacaa aaaatgtcgc
     5581 cgtatggtac ttcgggagct cgagtccagg caacacgtac atggaccccg ttgtcgacag
     5641 tgccgccagc ccggcaacaa tcaccttcat cggcggcaag ctggacacgg gcaatcccac
     5701 tgcgggcgtc accggcatgc gggtgctcct gggatcggtg aggttcgtgc gtacggaagg
     5761 cagtctcggc ctgggaccaa ccgcggagac ctacttcggc atcacggcat cgctgggaag
     5821 gcggagcccc tatgacaact tcgtcaccac gggcggccag gtcag
//
LOCUS       DAOPBQ010000195         5842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11903_length_5842_cov_4.512355, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000195 DAOPBQ010000000
VERSION     DAOPBQ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11903_length_5842_cov_4.512355"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..5488)
                     /locus_tag="PKW60_07600"
     CDS             complement(320..5488)
                     /locus_tag="PKW60_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPJ99337.1"
                     /translation="MSQVDALKMVEHVRTRLVHLALSENHFRAQNIKDAAHAVWAGPG
                     NEGGLVSELWVEGAYSGQLTEDTLDSLRAEGLLPENLHRHLSDNPHEVFPLNRRLFAH
                     QSEALRAAASGEEERPALVISAGTGLGKTEAFLLPALSELWSAPDRRPGGGMRCLLLY
                     PMNALVTDQVDRVYKWLQGQSAFRVFHFTSETPEDKRKADKQNEENWDACRARTRQEA
                     RGRETRDGRPIEEPLFAPVPDIVITNYSMLEYMLCRPQDQCFFGPDLRCIILDEAHLY
                     TGTLAGEIKMLLRRVRERCGVRARDVLHIATSATLGGSEEQLQDFAAKLFSTELSATK
                     VISGRHARHKFEGEVAPPSVEIDVAQLAAFADADFATLDAEGELITNDSTTVAALAKV
                     SRLVVSEHTVRLAEADSPSTPAQFLFRALQASPVVRNLEDILYNESGNVISLEALARR
                     LFRRSDLPAREATITLLRLTASARHRSGDLPIVPHRLHFLVRAPEGLSLCLNPQCSGP
                     SELKLDAVGCLQPCGELCRHCAHRLFAVHRCDNCGEVALAAFRDMESGALRPVFREQP
                     GQSAPTTYFLLHPDPQEELCVTVVDTATGEEIGRGDGITLWEAQREQPNGPQHCPTCA
                     STWSLGEDGERAVCRSLSEGRQFALSVIAETVLHDLPVYPDDTRHWKPGEGRRLLCFS
                     DSRSSAARLGPLLTIQHETQVVRAAMARLADELVPEEAADYARREVQRLDGELQRAQN
                     AGLRARLQRQLEDARRELRDAEAGTPFIDFVRLLRGREETAQLIERTLAEHHQKDFDQ
                     RAWNANRDAVREHAEALAGREVWRPRKKEALLESTGLIEVVYPGIAAIELPPQLEPHL
                     PRDLRSTLRAAWPHVVALMLDSVRAAGCIDWTSERQGLRWMDESPLQGRWLTRERNGW
                     NASPFVGATMRQTRRWFVAKVLGSAGCSEADAERLSELALGALFDHLCEQARNGPLHW
                     LRFVAAHQVNEITASPALQILMEGLSVRKPSRLFRCETTGTLWAHAALGWIPEKGCLG
                     RATEVDQATLDEDQRWGRARRELRESSIFQMGLWAEEHSAQLNPRENKRLQELFKRGA
                     RNVLSATTTMELGIDIGGLNGVLLSNVPPGPANHRQRAGRAGRRSDGSAVVVTYVRDT
                     NFERQAFLRFDEFLRRPLRVPTVVVNRPPIIRRHLHGMLLSEFLRPHQGDHAGAMHAF
                     GMMGHFCGEQAPDYWRRGSKPAWPASQPSLAAGFEAYLRGHEADAHLRQRLIQMTEGT
                     ALHDLEDDAIWEAFIAGAQKSFQDAAEGWRGQLADLRDAWNEIPGNPQANARRERSKA
                     ASIWYMADALCGTTVIAWLADKGFLPRYGFPINLQSLSVRSTGDEHGASRAEERYRLE
                     RNSLLALREYVPDAQVLAGNRLITSRGLRKDWTDANIDQSLGLSYHALTCPDGHVYVH
                     QNRNQACPVCQAPPAVRDLLVMPRFGFTTASWDSTPFGTRLERVGDPDYLPLAFVATA
                     QDEDHPDFLEVPGLHVWYRDEAEMLVRNRGKSGHGFAICHRCGYATSEERQGNGRVGL
                     PEGFALHARIFSTDRRDRCWRRDEEAPVLRNRVLAARDKTNLLLVRWPNFACTEALYT
                     LGRALVVAGAALLELDERELGLEPHVQLQDGRTGLALYDTAAGGAGHCRELLSRGHAW
                     FGRAREILYVDREHDARCERACLECILDFSSQRQAHLLNRVAALALLRDIGITG"
     gene            complement(5485..>5842)
                     /locus_tag="PKW60_07605"
     CDS             complement(5485..>5842)
                     /locus_tag="PKW60_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99338.1"
                     /translation="SKNRSEIIKLLCAFLCVRLGLDLRVDALEPWPPRGIVARIGRRL
                     RFHEAHVAGIVGMDAESLYDLASRWIDAAHHKEQLDQTDTATLRRVAEYNSVRNYFAC
                     RLCAHWLNATGMGIQL"
BASE COUNT         1157 a         1829 c         1672 g         1184 t
ORIGIN      
        1 tcggcaagcc agtcctgtac ggcctttgcc accagctcgg gaccgttgtc tgagcaggtg
       61 tatcgtggcg caccgtgaat cagaaagagt cactcgggtg cctgagcagg gcgcgagttc
      121 gggttgtccg tgtttggtga tacgtgtgtt cggaagccgt tccagtactc gatgtatccg
      181 gcaagaatcc gtctcggatg gctttcgccg aagacgatga catggtcgag acatttcgcg
      241 ccggatactc acgttaccgg ggcagtctcc ttgcctcatg cacgtcatca actgtggacc
      301 atgaagagtg aagattgctt taaccggtta tgccaatgtc acgcagcagc gcgagcgcgg
      361 cgacgcggtt caagaggtga gcctgacgtt gactcgagaa gtcaagaata cactcaaggc
      421 aggctcgttc gcagcgcgcg tcatgctccc tgtccacgta tagaatttcc cgcgcccgcc
      481 caaaccaggc atggcccctg gacaaaagtt ctctacaatg ccccgccccg ccggcggccg
      541 tgtcgtagag cgccagaccg gttcttccat cctgtagttg cacgtgtggt tccagcccga
      601 gttcacgttc atcgagttcc agcaacgcgg caccagccac gaccaacgcg cgccccaagg
      661 tgtacaaggc ctccgtgcaa gcgaagtttg gccagcgcac aagtagcagg tttgtcttgt
      721 ctcgtgcagc aagaacgcgg ttgcgcagaa ctggagcttc ttcatcccgg cgccagcatc
      781 tgtcgcggcg gtcagtactg aatatccgag catggagtgc aaatccctca ggcaacccga
      841 cgcggccatt gccttggcgt tcttcggagg ttgcatatcc acagcggtgg catatggcga
      901 atccatgacc agacttaccc cggtttctta ctagcatctc ggcctcatcg cgataccaca
      961 catgcaaccc cggtacttcc aggaagtcag ggtggtcttc gtcttgggcc gttgccacaa
     1021 aagccagcgg aaggtagtca ggatcaccaa cgcgctcaag gcgcgttcca aacggcgtgc
     1081 tgtcccaact tgcagtcgta aacccaaagc ggggcatgac cagaaggtct ctcacggccg
     1141 gcggcgcctg gcacaccggg caggcttggt tccggttctg atgaacgtaa acgtggccgt
     1201 ctgggcatgt gagtgcgtgg tagctcaacc ccaaggattg atcgatgttc gcgtccgtcc
     1261 aatccttgcg caatcccctg gatgtgatga ggcggttccc cgccagtacc tgcgcgtcgg
     1321 gaacgtactc tcgtagcgcg agaagtgaat ttcgttccag gcgataacgt tcttccgccc
     1381 gcgatgctcc gtgctcgtcg ccggtgctcc gtacacttag actttgcagg ttgatgggga
     1441 atccataccg ggggagaaat cctttgtcag caagccacgc gatgacagta gtgccgcaca
     1501 gcgcatcggc catgtaccaa atggaagccg ccttacttcg ctcacgacgg gcatttgctt
     1561 gaggattgcc gggtatttca ttccaggcat cccgcaagtc cgccagttga ccacgccatc
     1621 cctctgcagc atcctgaaaa ctcttttgag cgccggctat gaacgcttcc cagattgcgt
     1681 catcctctaa atcatgcagg gcagtgcctt ctgtcatttg tatcaaacgt tgccgcaagt
     1741 gcgcgtccgc ctcgtgccct cgaagataag cttcaaaccc agcggcgagg ctcggctggg
     1801 aagcgggcca tgccggcttg cttccccgcc gccagtagtc aggcgcttgc tcgccacaga
     1861 agtgccccat cattccaaag gcgtgcattg cacccgcgtg gtcgccttgg tgggggcgca
     1921 aaaattccga caatagcatg ccgtgcaagt ggcggcggat gatgggcggc ctgttcacga
     1981 cgaccgtcgg cacccgcagc ggacgtcgca gaaattcatc aaacctgaga aatgcctggc
     2041 gttcaaagtt tgtatcgcgc acataggtca cgacgacggc ggaaccatcc gaccgcctcc
     2101 ccgcccggcc cgcgcgttgc cggtgatttg ccggccctgg cggcacgttg gacagcagta
     2161 cgccgtttag cccaccgatg tcgatgccga gctccatcgt tgtagtggcg gagagcacgt
     2221 ttcgcgcgcc ccgcttgaaa agttcctgaa gtcgtttatt ttcgcgcggg ttcagttgcg
     2281 ccgaatgctc ctcggcccat agccccattt ggaatatcga cgactcccgc agttcgcggc
     2341 gcgcgcgccc ccaccgttgg tcctcgtcca gcgtcgcctg gtccacttcg gtggccctgc
     2401 cgaggcaacc tttctcggga atccaaccga gcgcagcatg ggcccacagg gtgccggtgg
     2461 tttcgcagcg aaacagccgt gatggcttcc ggacgctcag gccctccatc aggatttgga
     2521 gcgcgggaga ggcagttatc tcgttcacct gatgcgcagc cacaaacctc agccagtgca
     2581 gtgggccgtt ccgggcctgc tcacaaagat ggtcgaacaa cgccccgagt gcgagttcgc
     2641 tgagccgctc agcgtctgcc tcgctgcagc ccgctgaacc cagcaccttt gccacgaacc
     2701 atcgccgtgt ctgcctcatc gttgccccca caaaagggct ggcattccaa ccattgcgtt
     2761 ctcgcgtaag ccaccggccc tgcagcggtg attcgtccat ccacctcagc ccctggcgct
     2821 cactcgtcca atcaatacac ccagcggcac ggaccgagtc cagcatgagc gccaccacat
     2881 ggggccaagc ggcgcggagt gtgctccgca aatcgcgggg caggtgcggt tccagttggg
     2941 gaggaagctc gatggcggcg atgccgggat acacaacctc gatcaagcca gtggattcca
     3001 gcagcgcctc cttctttcgt gggcgccata cctcccgtcc agccaatgct tcggcatgct
     3061 cgcgcaccgc gtcccgattc gcattccagg cccgctgatc gaagtccttc tggtggtgct
     3121 ccgcgagcgt acgctcaatt agctgcgccg tctcctcacg accacggagc agtcgcacaa
     3181 agtcgatgaa aggcgtgccc gcctctgcat cgcgcagctc tctgcgggcg tcctccaatt
     3241 gccgttgtag ccgagcccga aggcctgcat tctgcgctct ttgcaattcg ccgtctagtc
     3301 gctgaacctc tctgcgggca taatcagccg cctcttccgg cactaattcg tcggcaagac
     3361 gcgccatggc cgcgcgcacc acctgcgttt cgtgctgaat cgtgagcagg ggcccaagcc
     3421 gcgcggcaga agagcgcgag tcactgaaac acaacaacct gcgaccctca cctggcttcc
     3481 agtggcgggt atcatcaggg taaaccggaa ggtcgtgaag caccgtttcc gcgatgaccg
     3541 acagcgcgaa ttggcggcct tcggacaacg aacggcaaac cgcgcgttcg ccatcttcac
     3601 caagactcca cgttgacgca caagtcgggc agtgttgggg accgtttggt tgctcgcgtt
     3661 gcgcttccca cagcgtgatc ccgtcgccac ggccgatttc ttcgccggtg gccgtgtcaa
     3721 caacagtcac acagagctct tcttgcggat cgggatgaag caggaagtac gttgtcggag
     3781 ccgactgccc aggctgctct cggaagacgg gccgaagtgc gccggattcc atgtcgcgga
     3841 atgcggccag ggcaacttcg ccgcaattgt cgcaacggtg gacagcgaag agacggtgtg
     3901 cgcagtgccg gcagagctct ccgcaaggtt ggaggcagcc cacggcatct agcttcaatt
     3961 cggaggggcc actacattgc ggattaagac acaaggagag tccctccggc gcacgcacaa
     4021 gaaaatgcag ccgatgtggg acaattggca gatcaccgga tcgatgtcta gccgaggcgg
     4081 tcaatcgcag gagtgtgatc gtggcttctc gcgcaggcag gtcgctgcgc ctgaatagcc
     4141 gtcgtgccag cgcctcaagg ctgatgacat tgccgctttc gttatacagg atatcctcaa
     4201 gattccgcac aacaggagag gcctgtagcg cacggaagag gaattgcgcg ggagtggacg
     4261 gagaatccgc ttcggccaac cgcaccgtgt gttccgaaac cacgagcctt gagactttcg
     4321 ccaatgccgc gaccgtggtg ctgtcattgg tgatcaattc accttcggcg tcgagcgtcg
     4381 caaaatccgc atccgcaaaa gcggccaact gcgccacgtc tatctcgacg gaagggggcg
     4441 ctacttctcc ctcaaacttg tgacgagcgt gtcgaccgct aatcactttg gtggccgaaa
     4501 gttctgtgga gaacaacttg gctgcgaaat cctgcagttg ttcctcgctt cccccgagcg
     4561 tggcggaggt ggcgatgtga aggacatctc ttgctcttac accacaacgc tcgcggacac
     4621 ggcgcagcag catcttgatt tcgcccgcga gggtgcctgt gtatagatgc gcttcgtcga
     4681 gaataatgca gcggagatcc ggcccaaaga aacactggtc ttgtgggcgg cacagcatgt
     4741 attcgagcat cgagtaatta gtgatgacga tgtccggcac gggtgcaaac agcggttctt
     4801 ctatgggtct gccgtcgcgc gtctctcgcc cacgggcctc ctgccgcgtg cgcgcgcgac
     4861 atgcatccca gttctcctca ttttgtttat ctgccttcct cttgtcttcg ggggtctcgc
     4921 tggtgaagtg gaagacacgg aatgccgact gcccctgaag ccatttgtag acccgatcaa
     4981 cctggtccgt aactagtgcg ttcatcgggt acaggaggag gcaacgcatc cctccgccgg
     5041 gccgtcgatc cggtgccgac caaagctccg acagcgccgg taacaagaac gcctctgtct
     5101 tacccaggcc ggtccccgcg gagatcacca gggctgggcg ctcctcttcg ccgctcgctg
     5161 ccgctcgcaa cgcttccgac tgatgagcaa acagcctccg gtttagtggg aagacctcat
     5221 gagggttatc agacaagtgc cggtgcagat tctccggcaa cagaccttca gcgcgtaggg
     5281 agtcaagggt gtcctcggtc agttgaccgg agtacgcgcc ctccacccac aactcgctaa
     5341 ccaagcctcc ctcattcccc ggccctgccc acacagcatg cgcggcatct ttaatgttct
     5401 gagccctgaa atggttttcg gacaacgcga ggtgcaccaa tcgcgttcgc acatgctcga
     5461 ccatcttcag tgcatcaact tgactcacag ttggattccc atcccggtgg cgttcagcca
     5521 gtgcgcgcaa agacggcaag cgaagtaatt gcggacgctg ttgtattctg cgactcttct
     5581 cagcgttgcc gtgtcggtct gatcaagttg ctccttatgg tgagctgcat ctatccatct
     5641 gcttgccagg tcgtacaagg actccgcatc catgcccaca atgccagcca catgtgcctc
     5701 gtgaaaacgc aatcgacgac cgattcgcgc cacaatgccc cgagggggcc agggttcaag
     5761 tgcatccaca cgcagatcga gaccaagacg cacacagagg aatgcgcaga gcaacttgat
     5821 tatttcagat cggttctttg ac
//
LOCUS       DAOPBQ010000196         5838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_11912_length_5838_cov_3.599516, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000196 DAOPBQ010000000
VERSION     DAOPBQ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11912_length_5838_cov_3.599516"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="PKW60_07610"
     CDS             <1..1494
                     /locus_tag="PKW60_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPJ99339.1"
                     /translation="RRHIEKDAALARRFQTILVDAPSVDQTIEIIHGLRDKYEAHHRV
                     KFSDEAIVAAARLSNQYISDRFLPDKAVDVIDEAGSRARLQITTRPAELKQVEKDIEV
                     VTQEKEAAIRSQEYERAAKLRDRERQLIGQLEEKKREWEKKRDSAEMVVTEEDIAYIV
                     SKWTGIPLTKLEENESHRLLRMREELRKSVVGQEGAIDSIARAIQRARAGLKNASRPV
                     GSFLFLGPTGVGKTLLAKTLASFLFGNDEALITVDMSEYMEKFAVSRLGGAPPGYVGY
                     NEGGQLTEQVRRRPYSVVLFDEIEKAHPDVFNVLLQVLEEGHMTDSTGRKVDFRNTVV
                     VMTSNIGTRRIGRSTAVGFQQSSADDEFKHMESRVMDEVKKVFNPEFLNRIDEMIVFH
                     RLGHAEMCQIVDIQVAEVIERLREKSLDLSLTESAREFIVRVGSDEQYGARPLRRAVQ
                     HHIEDPLSELLLKGEFGAGTRITVRPSETDDRLVFEPAGTVAEGVIT"
     gene            1494..3797
                     /gene="bamA"
                     /locus_tag="PKW60_07615"
     CDS             1494..3797
                     /gene="bamA"
                     /locus_tag="PKW60_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPJ99340.1"
                     /translation="MRLTIYPVLAVALAVCAGAAAQPADLAGKPLGDVRLQGLERISE
                     QVVRAQIESQPGGTYSPRAVARDLRRLYELGYFATIKADAAVEANKVVLTYVFEEKRV
                     ISELRIVGNDKVRTRHVRAALTWSEGDTFDEQGYAAERQSVLDLYESKGFPNAQVNFI
                     VEEAGPGRVNVTCAIEEGRKARIRKIAFDGNTALSRRKLKKLVKTKRAWWFLGGKYEE
                     ATLESDLANILAEYKNYGRLEADIPEVDFAYDDSGKRVDLTIHIEEGPEYTVSALDTA
                     GNVTFDDDEVMRLLQVQAGDVHNAGQVTADAQLVQKGYEDSGFVTASVEPQVTLDRDA
                     KTTHIIHRVQEGDLKYVGEIKITGNTVTQDDVVRRELMLYPGERFDGTMLRASERRLD
                     NIQYFDSVRFTREPIPGDDVFENLLVDVEEGKTGNFDFGLGYSTEEKFGVYSELRLRN
                     FDLANWPRFSGGGQQLRLRLNVGELRNQYYLAFTDPEIFGYPLAFGFDVFDESREYET
                     GLNYKESNQGLQLRLGKQLSPYVSVRTALRYSDVDINDYGFVLNPEIRRWGGDVTVSS
                     LWGINRNTVDRRFDPSRGSEHDLGFEFAGFGGDNEFYRIDHDSIWYVPIDKAEKWIFS
                     FRTREGWVEDYGDPGYVPIQYRYFAGGSSTVRGFDYREIGPTERRFFWFGDKAYVGGK
                     LRVVQNAEIKYKITENLRVYSFLDAGGVWRDASDFDLGDMKYSIGAGLGFNVPRLGPI
                     RLDYGIPLNPDADQGSGRLHLQTGLKF"
     gene            4039..4614
                     /locus_tag="PKW60_07620"
     CDS             4039..4614
                     /locus_tag="PKW60_07620"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPJ99341.1"
                     /translation="MKYRAMMLGLILGVTITAALLLNGHVLAQDDAGAAWKIAVVDRK
                     AVFTEYKKQQDEMKKLQSQLDGMQKELDEISKRIQGAKDAYTENREKMSEAERDAEKS
                     RIQQEFVKYEAELKSRQAQMDAKTAALIKEVKGDIDAAIAKYGEENRYHLILESDSDP
                     QSRTAVLYYHSKIDITLQIQQILNDAYAKGR"
     gene            4674..5726
                     /gene="lpxD"
                     /locus_tag="PKW60_07625"
     CDS             4674..5726
                     /gene="lpxD"
                     /locus_tag="PKW60_07625"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPJ99342.1"
                     /translation="MNVTVGEIAALVGGCVIGDKSVRLTGVNGIRQAGEGDLSFISSA
                     RYMRYLDESRASALLVPPAIGEARMPLIQVAEPYAAFLSVLDRYLPAREGHPQGIHPS
                     AAIGEHVELGQNVAVDAHVTVAAHAVLGDNVCLHAGVYVGPGARIGPDTVIYPNASIL
                     AGVQLGARCIIHSGAVIGSDGFGYASGAAGHRKIPQVGNVILGDDVEVGANSAIDRAT
                     FGSTVIGNGTKIDNLVQIGHNVEMGSACIVCGNAGISGSAILGHGVTIAAGAGVAGHL
                     EIGDGAVVAGLSGVTKSIEPGKVVSGFPAMDHERDKRILVSMRHLPEALHEIRALRRR
                     VEELEKLSNGTPKNHS"
     gene            5701..>5838
                     /locus_tag="PKW60_07630"
     CDS             5701..>5838
                     /locus_tag="PKW60_07630"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPJ99343.1"
                     /translation="MERQKTIAKPCSFSGIGLHTGNLTTLTFKPAEPGKGVTFYRIDL
                     PE"
BASE COUNT         1273 a         1632 c         1822 g         1111 t
ORIGIN      
        1 cgccgacaca tcgagaagga tgccgcactg gcccggcgct tccagaccat cctggtcgac
       61 gcgccgtccg tcgatcagac catcgagatt atccatggtc ttcgcgacaa gtacgaggcg
      121 caccaccgcg tcaagttttc cgatgaagcc attgttgctg cagcccggct gtccaaccag
      181 tacatcagcg accggttcct gcctgataaa gccgtggacg tgattgacga ggcggggagc
      241 cgcgcccggc tccagataac cacccggccc gccgaactca agcaggtcga gaaagacatc
      301 gaggtagtga cccaggagaa agaagccgcc atccggagcc aggaatacga acgtgctgcc
      361 aagctgcgcg accgcgaacg ccagctgatc ggccaattgg aagagaagaa gcgcgagtgg
      421 gagaaaaagc gcgattcagc cgagatggta gtcacggagg aagatatcgc gtatatcgtc
      481 tccaaatgga ctgggatccc gctgacgaag ctcgaagaaa acgagtcgca ccgcctgttg
      541 cgcatgcggg aagagctgcg caaatcggtc gtgggccagg aaggcgccat tgacagcatt
      601 gcgcgggcca ttcagcgggc gcgggcaggg ctcaaaaatg cgtcgcgccc ggtgggttct
      661 tttctgttcc tggggccgac gggcgtgggc aagacgctgc ttgccaagac cctggcttcg
      721 ttccttttcg gcaacgacga ggccttgatc acggtcgaca tgtccgaata catggaaaaa
      781 tttgccgtgt cgcgcctggg cggcgctccc cccggctacg tcggatacaa cgaaggcggc
      841 cagctgaccg agcaggtgcg gagacggccc tattcggtgg tattgttcga cgagatcgaa
      901 aaggcccacc ccgatgtgtt taatgtgctg cttcaggtcc tcgaagaagg gcacatgacc
      961 gattcgaccg gaagaaaggt cgactttcgc aacacggtcg tagtgatgac gagcaacatt
     1021 ggcacgcgcc ggatcgggcg gagtacggcg gtgggtttcc agcagagctc ggccgatgac
     1081 gagttcaagc atatggaatc ccgggtgatg gacgaggtca agaaagtctt caatcccgag
     1141 ttcctgaacc ggatcgacga gatgatcgtg ttccaccggc tgggtcacgc cgaaatgtgt
     1201 cagattgttg acattcaagt ggcggaggtg atagaacgcc tgcgggaaaa gtcgctcgat
     1261 ctgtccctca ctgaaagtgc gcgagagttc attgtccggg tgggcagcga cgagcagtat
     1321 ggcgcgcggc cgctccggcg agcggtacag catcatatcg aggatccgtt gtccgagttg
     1381 ttgttgaagg gggaattcgg cgctggaacc cgtatcacgg ttcggccgtc ggaaaccgac
     1441 gacaggctgg tgtttgagcc ggccggtaca gtggcggaag gtgtaatcac ttgatgcgtc
     1501 ttaccatcta tccggttctt gccgttgccc tggcggtgtg cgcgggcgcg gcggcccagc
     1561 ccgcggacct ggcggggaaa cccctcggcg acgtccgttt gcaggggctc gagcgcatca
     1621 gcgaacaggt ggtgcgcgcg cagatcgaat cccaacccgg cggcacctac agcccgcgcg
     1681 ccgtggcgcg cgacctgcgc cgcctctatg agcttggcta ttttgccacc atcaaagcgg
     1741 atgccgccgt cgaagcgaac aaggtcgtgc tgacctacgt cttcgaggaa aagcgcgtga
     1801 tcagcgagct gcggattgtc ggcaacgata aggtgcggac ccggcatgtg cgggccgccc
     1861 tgacgtggag cgaaggagac acgtttgacg aacagggcta tgccgccgaa cggcaaagcg
     1921 tgctggacct gtatgaaagc aaagggttcc ccaacgccca ggtgaacttc attgtcgaag
     1981 aggccggccc cggccgcgtc aacgtcacct gtgcgatcga ggaggggcgg aaggcacgca
     2041 tccgcaagat tgcgtttgat gggaacaccg cgctttcccg ccgcaagctg aaaaaactcg
     2101 tcaaaaccaa gcgtgcctgg tggttcctcg gaggaaaata cgaggaggcc acgctggaat
     2161 ccgacctggc gaacatcctg gcggaataca agaactatgg gcggcttgag gcggatatcc
     2221 ccgaagtgga tttcgcgtac gatgactcgg gcaaacgggt ggatctcaca atccacatcg
     2281 aagaaggtcc ggaatatacc gtgtccgcac tggacacggc cggcaatgtc acgttcgacg
     2341 acgacgaggt gatgcgcctg cttcaggtcc aggcgggcga tgtgcataac gcggggcagg
     2401 tcactgcaga tgcccagctt gtgcagaaag gttacgaaga cagcggtttt gtcaccgcat
     2461 ccgtcgagcc gcaagtgacc ttggatcgag acgccaagac cacgcatatc atccaccgtg
     2521 ttcaggaagg cgacctgaag tacgtgggcg agatcaaaat caccggcaac accgtgacgc
     2581 aggacgatgt ggtgcggcgt gagctgatgc tgtatcccgg agaacgtttt gacgggacca
     2641 tgctgcgggc cagtgaacgc cggcttgaca acatccagta cttcgattcc gttcgattca
     2701 cgcgcgaacc gataccgggc gatgacgttt tcgagaacct gctggtggat gtggaagaag
     2761 gaaagaccgg caactttgat ttcggcctgg ggtacagcac agaagagaaa ttcggggtgt
     2821 attcggaatt gcggctgcgc aattttgacc tggcaaactg gccgcgtttc agcggaggag
     2881 gccaacagct tcggctgcgc ctcaacgtcg gcgaacttcg caaccagtac tacctggcat
     2941 tcactgaccc cgagatcttc ggttaccccc tggcgttcgg atttgacgtt ttcgacgaat
     3001 cgcgtgagta tgaaaccggc ctcaactaca aagaatccaa tcagggcctg cagctgcgcc
     3061 tcggaaaaca gctgtccccc tatgtgtcgg ttcgcaccgc tctgcggtac agcgacgtgg
     3121 acatcaacga ttacgggttc gtactgaatc ctgaaatccg ccgctggggc ggggatgtga
     3181 ctgtgagttc cctgtggggc atcaaccgca acaccgtgga ccgccgtttt gatcccagcc
     3241 ggggctctga gcacgacctt ggatttgagt tcgccggatt cggcggtgac aacgagttct
     3301 accggatcga ccacgattcg atatggtatg tccccatcga caaagccgag aaatggatct
     3361 tctccttccg gacccgggaa ggatgggttg aggactacgg cgacccgggc tacgtgccca
     3421 ttcagtaccg gtatttcgcg ggcggttcgt caaccgtacg cgggttcgac taccgcgaga
     3481 tcggccccac cgaacggcgc tttttctggt ttggggacaa ggcgtatgtc ggcggcaaac
     3541 tgcgcgttgt gcaaaacgcc gaaatcaaat acaagattac ggaaaacctt cgtgtctatt
     3601 cgttcctcga cgccggcggc gtgtggcgtg acgccagtga ttttgacctg ggcgacatga
     3661 aatacagcat cggcgccggg ttgggtttca atgtcccgcg gcttggtccc atccgtctcg
     3721 attacgggat tccgcttaat cccgatgcgg accaggggag cggacgcctg catctccaga
     3781 cgggtctgaa attctagttc cgtattcgcg acggtccggc gtcagcctgc cctggcggca
     3841 gagacggcct gggccggagt gaccatcccg ggagggtcat gccgtttttc aaagaggagt
     3901 ttttcccgga tgcgaatggc ggtttgcggc gtttcgagcc gtaatgatat actgccgttc
     3961 ccgtgtgctt agcgcgccgg ccttgacgag cttgaacctg aaacatctaa caaatgatta
     4021 cagaggggat ttggtgccgt gaaatatcgt gccatgatgc tggggttgat tcttggagtc
     4081 accataacgg cggcgttgct cctgaatggc catgtgcttg cgcaagatga cgcgggtgct
     4141 gcctggaaga tcgcggtggt cgaccgtaag gcggtcttca ccgaatacaa aaaacagcag
     4201 gacgaaatga agaaactgca gtctcaactc gatggcatgc agaaagaact ggatgaaatt
     4261 tccaagcgca ttcagggtgc taaagacgcc tataccgaaa accgcgaaaa gatgtccgaa
     4321 gcggagcgcg atgccgaaaa gagccggatc cagcaggaat ttgtaaaata cgaggcggaa
     4381 ctcaaatccc gccaggcgca gatggacgcc aagaccgccg cgcttatcaa agaggtcaaa
     4441 ggcgatattg acgcggccat tgccaagtac ggggaagaga accggtacca tctgattctt
     4501 gaaagtgatt ccgatcccca gagccgcacc gcggtgctgt attaccactc aaaaattgac
     4561 atcacgcttc agattcaaca gattctgaac gacgcctacg cgaagggccg ttaaccgatg
     4621 acgtcctgtg cgcctgtccg ggggcatgtg cggcagggac cacgggtatg tgaatgaacg
     4681 tgacagttgg cgagatcgca gcgctggtcg gcggctgcgt aatcggagac aaatcggttc
     4741 gccttacggg cgtaaacggc attcgccagg ctggtgaagg ggacctttcc ttcatctcca
     4801 gtgcgcgata catgcggtat ctcgatgaat ccagggcctc ggcgcttctg gttccaccgg
     4861 ccattggcga agcccgcatg ccgctgattc aggtggcgga accctacgcg gccttcttga
     4921 gcgttctcga ccgctatctg cctgcccgag aaggacatcc ccagggtatt cacccctccg
     4981 cggccattgg cgaacacgtg gaactcggcc agaacgtcgc cgtggatgcc catgtcaccg
     5041 tcgccgcgca tgctgtgctt ggcgataacg tgtgcctgca tgcgggcgtg tacgtggggc
     5101 ccggggcgcg aatcggaccg gataccgtca tctacccaaa tgccagcatt ttggcggggg
     5161 tccagctggg cgcccgctgc atcattcaca gtggcgcggt catcggctcc gacggatttg
     5221 gttatgcctc cggcgccgcc ggccaccgga agattcccca ggttggaaat gttatactcg
     5281 gggatgatgt ggaagtgggc gccaactcgg ccatcgaccg ggcgactttt ggaagcaccg
     5341 tcatcggaaa tggcacaaaa attgacaacc tggtgcagat cggacacaat gtcgagatgg
     5401 gttctgcatg catcgtctgc ggcaacgcag gcatttccgg cagcgccatt ctcggccatg
     5461 gggtgaccat cgccgccggg gccggagtgg cgggccatct ggaaattgga gacggagccg
     5521 tcgtcgccgg cctgagcggc gtgaccaaat cgattgaacc cggcaaagtg gtctccggct
     5581 tccccgcgat ggaccacgag cgcgacaaac gtattctggt gagcatgcgc catctccccg
     5641 aagccctgca cgagattcgt gccttgaggc ggcgggtgga ggagttggaa aaactgtcga
     5701 atggaacgcc aaaaaaccat agctaaaccg tgttctttct cgggcattgg cctgcacacc
     5761 ggaaacctga ccacactcac gttcaaaccc gccgaaccgg gtaagggcgt caccttctac
     5821 cgcatcgacc tgcccgag
//
LOCUS       DAOPBQ010000197         5798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12015_length_5798_cov_3.897963, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000197 DAOPBQ010000000
VERSION     DAOPBQ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12015_length_5798_cov_3.897963"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="PKW60_07635"
     CDS             <1..1679
                     /locus_tag="PKW60_07635"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HPJ99344.1"
                     /translation="KEAPTDTRYNQVHLPGVNARGTNFVATVDAVYIIQGKETHVLDA
                     VSGATKHIFRLPPIDPEAKKKQYPDWGYIAVSGDLLIGGAHFVAFSDVAPVKSAEYSI
                     WSDYDCTASRELVVMDRHTGEVLWTVSSRHGFLHNGIAIGNGTLYCLDKMPPAIGDRL
                     RRRGIEPETSPRLLALDLQSGEIRWETEENVFGSFLSYSEEHDLLLQATRPSRDRLEG
                     EEGKRMIAYHGQDGQIIWDKEVEYATFPILHNDRIITETTCLQLATGDVLTREHPLTG
                     EVQAWGWTRAYGCNYPIAAENMLTFRSGAAGFYDLLHDGGTGNFGGFKSGCSNSLIIA
                     DGVLNAPDYTRTCSCAYQNQTSLAMIHMPEVEVWTFNSLTDPKGPIVRLGINLGAPGD
                     RRLDDGTLWLDYPDVGGPSPKLTVTAQPEDTHWFRHHSSRIQEGTYPWVAASGALGLQ
                     TLRVRLQPEGSEEKRYQIRLFFAENAGAEPAERVFDIRLQGDVAAEGVDVVALAGGPY
                     REHVLEFPGIAVTDDLVIELTPRSKLETLLCGVEAIAEDSSPEKVARAYE"
     gene            1672..2775
                     /locus_tag="PKW60_07640"
     CDS             1672..2775
                     /locus_tag="PKW60_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99345.1"
                     /translation="MSRRNQKPAWTDARHFARAVRPLFALTLALAPAVVVLACEIPVY
                     QWALLNWEPDVYELLVFHDEPLAPDIARALNELAEKASPAHRALNVVIRDLGPEADAG
                     EDVAQIRQREQAGDSPWMALRYPGMPPNIPSLWTVSASPETLGLLSRSSVRDRITNAL
                     LRGISHVWVVLESGDPAKDAAARERLESALATMAADIDPDTISPNAHITSEIVTLRRD
                     DPEEALLVQTLLRSEPDLFAYEEPMVFPVFGRGRALYALVGAGISADNLRDACAYVAG
                     RCSCLVKDENPGVDLLLSANWDGVAAAVADALPDSPEPTDAAVAEAIAAALPEPRLVP
                     GWYILAALAGALMLVGILSAVFLRRSYSPSASR"
     gene            2831..3304
                     /gene="sixA"
                     /locus_tag="PKW60_07645"
     CDS             2831..3304
                     /gene="sixA"
                     /locus_tag="PKW60_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HPJ99346.1"
                     /translation="MRLYLVQHGEAMEKRENPDRPLSARGRKDVAAVGHLLRQAGVSI
                     PLVEHSGKVRARETAELLARAVGSAAVEERKDLAPNDPVDIVSKSLKNMEHDLMLVGH
                     LPFMNKLASLLVTGDEYANVLTFQQGCVVCLEREVDATWQVAWMVTPELARAEGF"
     gene            complement(3371..4789)
                     /locus_tag="PKW60_07650"
     CDS             complement(3371..4789)
                     /locus_tag="PKW60_07650"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPJ99347.1"
                     /translation="MSEVCDQNKDDVRLSVTVDRLAVLFSGTVDEARQREIIAAIIEK
                     LTALGVTPVPSEAGLWKALSEATPDEDGRLTGVVLVAGTAPQPSKDGWIEWPRDFFSS
                     GFVVNEQTGAVNYRERIGDPTIQKGELIARVWPAEPGQDGQDVFGRCIPAATPKTLYV
                     RCGTNVEVSEDGIEYRATRSGRVRYSRQVLSVDRVYIIDGNVGLASGNIDHTGAVFVE
                     GDVEAEARVRSVGDVEVRGIVEGADIDAGGNLFVRRGITGGGHRHIKAGGRVQARFLV
                     ETNIEAGDDVWVERESINSTVFTKGSFIMPGGRLVGGSVVAQGSIRVRQAGSEGLVRT
                     RLAIELDPALTAAIGEKDAEIKRIRSSLMKIKETLSALKRKNSTLSDSAREALTLLAG
                     NIEDMRSTLATLESEREELLAQLHSRGRPQIIVRTVAYPETIFDIYTHRVRLRETLLG
                     PVRAVVGKKGIEFQAIALRAQS"
     gene            complement(5041..>5798)
                     /locus_tag="PKW60_07655"
     CDS             complement(5041..>5798)
                     /locus_tag="PKW60_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99348.1"
                     /translation="DTLFWQNHAPRNTISALQEYNREVDAAGCDIYPMPFNRGVLHSD
                     LPNRSLTCIGDYTRPMQAAAPGKAVWMVLQGFGWKDLNDPFNPKDDVGGRRPTFEESR
                     FMAYDALVHGAKALIYWGVHVLEPGDQLWLDLLRLARELRALEPGIVGEPPATPPACA
                     GDDTYASVGSEGPVLMLRRAGEDWILIAVNESYAGIGFEVSQLPPELNGKSLYRLYSN
                     ETITVQNNRFRDGIRGYDVQVYATSTRFEMSPE"
BASE COUNT         1106 a         1764 c         1809 g         1119 t
ORIGIN      
        1 acaaggaagc accaaccgac acccgttata accaggtcca ccttcccgga gtgaacgccc
       61 gcggcacgaa tttcgtggct acggtcgatg ccgtgtatat cattcaggga aaagaaactc
      121 acgtactcga tgccgtttcc ggggccacga aacacatttt ccgactcccg cccattgacc
      181 cggaagcgaa gaaaaagcag tacccggatt ggggatatat cgcggtctca ggcgacctgt
      241 taatcggagg cgcgcatttc gtggcgtttt cagacgtggc cccggttaaa tccgcggaat
      301 actcgatttg gtcggactac gactgcaccg ccagccggga actggtagta atggaccggc
      361 acacgggcga agtcctctgg acggtgagtt cgcggcacgg ctttcttcat aacgggatcg
      421 cgattggcaa cgggacgctg tactgcctcg acaagatgcc tccggccatt ggagaccgcc
      481 tccggcggcg cggcattgaa ccagagacct ccccccggct gctggcgctg gacctgcaaa
      541 gcggcgagat ccgatgggaa accgaggaga atgtgttcgg ctcgttcctg agttactccg
      601 aagaacacga cctcctgctc caggccaccc gtccgtcgcg cgatagactg gaaggcgaag
      661 aaggcaagcg gatgatcgcc taccacggac aggatggcca gatcatctgg gacaaagagg
      721 tcgagtacgc gacatttccc attctccata acgatcgcat catcacggaa accacgtgcc
      781 tgcagctggc caccggcgac gtgttgacgc gggagcatcc gctgaccggt gaagtgcaag
      841 cctggggctg gacgcgcgct tacggctgca actaccccat tgcggcggag aatatgctga
      901 cgttccggtc gggcgcagct ggtttctacg acctgctcca tgacggcggg acgggcaact
      961 tcggcgggtt caaatcgggt tgcagcaaca gtctcatcat tgccgacggc gtgctcaatg
     1021 cgccggatta cacccgtacg tgctcctgcg cctatcagaa ccagacctcg ctggccatga
     1081 tacacatgcc tgaggtggaa gtgtggacat tcaattcgct gaccgacccc aagggtccta
     1141 ttgttcgcct gggcattaat ctgggcgccc cgggcgaccg gcggctggac gacggtaccc
     1201 tgtggctgga ctacccggac gtcggcggcc catccccgaa actcacggtg accgcccagc
     1261 cggaagacac gcactggttc cgccatcact catcgcgcat tcaggaaggc acgtatccgt
     1321 gggtggcggc gtccggcgcg ctcgggctgc agacgctccg cgtccggctt caacctgaag
     1381 gctcggaaga gaagcgatac cagattcgac tctttttcgc cgaaaatgcg ggtgcggagc
     1441 ctgcggaacg tgtcttcgac atccgcctgc agggcgacgt cgccgcggaa ggcgtggatg
     1501 ttgtggccct ggcgggcggc ccctaccggg agcacgtgct ggaatttccg ggcattgccg
     1561 tgacggatga tctggtgatc gagctgacgc cgcgctccaa actggaaacc ctgctctgcg
     1621 gcgtagaagc catcgcggag gatagttctc cggagaaagt ggccagagcc tatgagtaga
     1681 cggaatcaaa agccggcctg gacagatgcg cgtcatttcg cacgggccgt gcggccgttg
     1741 ttcgcgctga cactggcgct ggcgccggcc gtcgtggtct tggcctgtga aatccccgtg
     1801 tatcaatggg ctttgttgaa ctgggagccg gacgtgtatg agcttctggt ttttcatgac
     1861 gagcctctgg cacccgacat tgcacgggcc ctgaatgagc tggccgagaa agcatcccct
     1921 gcccatcggg cattgaatgt agtgatacgt gacttggggc cggaggcgga tgcaggggaa
     1981 gatgtcgcgc agatccggca gcgtgagcag gccggggact ccccgtggat ggcgctgcgc
     2041 tatccgggca tgcccccgaa tattcccagc ctgtggacag tgagcgcaag ccccgagacg
     2101 cttgggctgc tctcccgatc cagcgtgcgg gatcggatca cgaacgccct gctccgaggg
     2161 atctcccatg tctgggtcgt gctcgaatcc ggcgatccgg ccaaggacgc cgctgcccgg
     2221 gagcggttgg aaagcgctct ggccactatg gccgccgata tcgatcccga cacgatatcc
     2281 cccaacgcgc atatcacgag cgagatcgtt acgctgcgcc gagacgaccc tgaagaggcg
     2341 ctgctcgtcc agaccctgct gcgcagtgag ccggatcttt tcgcatacga agaaccgatg
     2401 gtctttccag tgtttggcag aggccgcgcg ctgtatgcgc tggtgggtgc cgggatctcc
     2461 gccgataacc tgcgggacgc ttgcgcgtat gtagccggcc gctgttcgtg cctcgtaaaa
     2521 gacgagaacc ccggagtcga tttgctgctg agcgcgaact gggacggcgt cgcggcggcc
     2581 gttgcggacg cactgcctga ttccccggag ccgacggacg ccgcggtggc cgaggcgata
     2641 gcggcggctc tgccggaacc acggctcgtg cccggctggt acatcctggc agcattagcc
     2701 ggtgcgctga tgctggtagg catactctct gccgtgtttc tgcgccgttc ctattcacct
     2761 tctgcgtcgc gatagccctg atctgcagcc aattcacacg gcacggtaac ggccaacgga
     2821 gggttcgtgt atgagactgt atctggtgca acacggcgaa gcgatggaga agcgcgagaa
     2881 tccggatcgc ccgctctccg cacgagggcg caaggatgtg gccgcagtgg gccatctgct
     2941 gcgccaggcc ggcgtgagca tccctcttgt tgaacacagc ggcaaggtcc gcgcgcggga
     3001 aacggccgag ctcctggccc gcgccgtggg cagcgcagcg gtggaggagc gcaaagacct
     3061 cgctcccaac gatccggtgg acatcgtgag caaatcactg aaaaacatgg agcacgacct
     3121 tatgctggtc ggccatctgc cgttcatgaa caaactggcc tcactcctgg ttaccggcga
     3181 tgaatatgcc aatgtgctga cgtttcagca gggctgcgtc gtttgcctcg agcgtgaggt
     3241 ggacgccacg tggcaggtgg cctggatggt gactcctgag ctcgcccgcg cagaaggctt
     3301 ctgacggcag tctgcggagt gatactaaaa agaggcggcg gcgaatcgcg cctggcgcgt
     3361 tcgtggagcg ttacgactgg gcccgcagcg caatcgcctg aaattcgatg cccttcttcc
     3421 ctacaacggc ccgaaccggc ccgagcaggg tttcccgcag gcgcacacgg tgtgtgtaga
     3481 tgtcgaagat ggtttcgggg taagccacag tccggacaat gatttgggga cggccccggc
     3541 tgtgcaactg ggccagcagc tcttcccgtt ccgattcgag tgttgcaaga gtagagcgca
     3601 tatcttcaat gttgcctgcg agcagcgtga gggcctcgcg ggcgctgtcg ctcagcgtgc
     3661 tgttctttcg cttgagagca ctgagcgttt ccttgatctt catgaggctg ctgcggatgc
     3721 gcttgatctc agcgtccttc tcgccaatcg cagcggtcag tgcagggtcc aattcaatgg
     3781 ccagccgcgt gcgtaccagg ccctcgctgc cggcctgcct cacccggatg cttccctggg
     3841 cgacgacgct gccccccacc aggcggccgc ccggcatgat gaaggaaccc ttggtgaaaa
     3901 ccgtggaatt gattgattct cgctcaaccc aaacgtcgtc cccggcttca atgtttgttt
     3961 ccacgagaaa acgggcctgc acccggccgc ccgccttgat gtggcggtga ccgcctcctg
     4021 tgatcccgcg gcgcacgaac aagttgccgc cggcatcaat gtcagcgccc tcaacaattc
     4081 cccgcacctc gacatcgcca accgaacgga cgcgggcctc cgcctccacg tctccctcaa
     4141 cgaacaccgc gcccgtgtgg tcgatgtttc cagacgccag gcccacattg ccgtcgatga
     4201 tataaacccg gtcgacgctc agcacctgcc tggagtatcg gacgcgcccg ctgcgcgtgg
     4261 cacggtactc aatgccgtct tcgctgacct caacattcgt accgcaccgg acgtacaggg
     4321 ttttgggcgt ggctgcagga atgcacctgc caaacacgtc ctgcccgtcc tgtcccggct
     4381 cggccggcca cacgcgggcg atcaattccc ctttctgaat ggtgggatcg ccgatgcgtt
     4441 cgcggtagtt gacggcaccg gtctgttcat tcacgacaaa gccggaactg aaaaaatcgc
     4501 gaggccattc gatccagcca tcttttgatg gctgcggcgc cgtgccagcc acgagcacca
     4561 cgccggtcaa ccggccgtct tcatccgggg ttgcctccga caaggctttc cagagcccgg
     4621 cttcggaagg aacgggtgtg acgcccaggg cggtgagttt ttcaataatg gcagcgataa
     4681 tttcccgttg ccgcgcttca tctaccgtcc cgctgaaaag gaccgccagg cggtccaccg
     4741 tgacggagag gcggacatca tctttgttct ggtcgcagac ctcgctcatg atctgagata
     4801 agtgctgaaa tccacgtaaa ccctaccatt tacaaggcgc atcgtggatt ttcggcgtat
     4861 accagtttca actacaggcc cacttggtta taaacaaaag tgtgcaactt ttgcaaggta
     4921 cttgttgcgg gaggtgccag caccaaaaaa ggcctgtttc atgcaaggca gggcgtgagt
     4981 aatctagcac agtcatccga agctaacagc tgtgggggta gcggagcgtt ctctcttttc
     5041 tcactcgggc gacatctcaa agcgcgtcga tgtggcatag acctggacat catagcctcg
     5101 gatgccatcg cggaagcgat tgttctgcac ggtgatggtt tcgttggagt acaggcggta
     5161 gagggatttg ccgttcagtt cgggcggaag ttgtgacacc tcaaacccga tcccggcgta
     5221 ggattcgttg acggcgatca ggatccagtc ttcgccggcc ctgcggagca tcagcacggg
     5281 tccctcgctt ccaacagagg cataggtgtc gtctccggcg caggcaggcg gcgtggcggg
     5341 cggctccccg acaatgcccg gttccagagc gcgaagctcg cgggccagcc ggagcaggtc
     5401 cagccacaat tgatcgccgg gttcgagcac atggaccccc cagtagatca gagctttggc
     5461 gccatgcacc agggcgtcgt aggccatgaa gcgcgattcc tcaaacgtgg gccgccgccc
     5521 gccgacatcg tccttcgggt taaaggggtc gttgaggtct ttccagccga atccctgcag
     5581 aaccatccaa accgccttgc cgggcgcggc ggcctgcatg ggccgggtat aatcgccgat
     5641 gcaggtcaag ctgcggttgg gcaggtccga atggaggacg ccgcggttga atggcatggg
     5701 atagatgtcg cagcccgcgg catctacctc cctgttgtat tcctgcaacg cggagatggt
     5761 gttgcggggg gcgtggtttt gccagaagag ggtgtctg
//
LOCUS       DAOPBQ010000198         5795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12021_length_5795_cov_4.096167, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000198 DAOPBQ010000000
VERSION     DAOPBQ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12021_length_5795_cov_4.096167"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PKW60_07660"
     CDS             <1..747
                     /locus_tag="PKW60_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99349.1"
                     /translation="GCNDDGMMYDSLDQGKGQLSDGWGYNYVGYLCYDQATGTETYRP
                     QVVRTLRNLMKPLYENYAWEGESIDGYADSIEGALYLLNRVPVAEGFTWVDRETKNNL
                     VDHPSRLENGELWGTMKLQSNGVRTVLMHSLMHTRGVIARPWQQGLELGAAPRGEGLS
                     LVVRSGEAYRGVLEFDLPRYRVYLGFQKDWPRMNTLPEWFTVEPERNYTVTWVDTGAR
                     AIYTGRQLHCGLAIAVEAGETLRLTVDPAP"
     gene            complement(862..1911)
                     /locus_tag="PKW60_07665"
     CDS             complement(862..1911)
                     /locus_tag="PKW60_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPJ99350.1"
                     /translation="MPKIKGKYRFSFGPWNIDEGSDPFGPEVRKPIAMAKKFKAAKTM
                     GFHGIQFHDDDVVPDLDNKSHSQIMKEARALKRALDGEGLKAEFVAPRLWFATQTIDG
                     AYTSNNPRDRKYAIDRTKVAIDIKNAIDAQNMVMWLAREGTYIREAKNPRTQVLQIVE
                     AFNLFLEYDPKLRILIEPKPNEPVDSAIISTIGHAVGLGYMTCDPARMGVLVETAHSI
                     LAGLDPSDDMGYALAHDKLWSVHLNDQGGLKFDQDKSFGSYNLRRAFNQVRVLEEYGY
                     GRNGEWVGLDVKVMRTQKQNVSAKHLSNSLNTFKKLLDKVVSLDRKLEAQLIKERDYE
                     ELDRYILCHLMGVKY"
     gene            complement(2090..3547)
                     /locus_tag="PKW60_07670"
     CDS             complement(2090..3547)
                     /locus_tag="PKW60_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99351.1"
                     /translation="MNYEVNRRTFMISSAALVASGMLSQAHAAGKSALQAAPRSKKPA
                     RVRGAFFYPPKEVVLEGKCEDSWSKHQWFTWPGNQFEPEAQQAAFEQRLSDMTRDLSV
                     ELELERAPVFTDADIQAFIADITANKPDALLLFNFWNSFSAKIVPILDAYDGPIILYH
                     PLGANHQLPPERFRTGKGLHYIHSIEHWDALERGLRAIHTKARMAQSRLLRVSGRLEK
                     EAEDHVEFFDLPVHGVPAGYFNDLYDATAITPEMKRLAARIRRRADNIMDLEQKAFLD
                     AVRAHAAVLALMEKYDADAITIECLFLKHRKPCLSFSINNGALVPCGCENDLNATLTL
                     MLGANLFGRGGFQHNPEFDTEENLYFASHCTCTTRLHGPGAKETSYTLRPFFHQMPKT
                     LALDVNWPAGERITLAKYHRDENRLDAWAGDIIDSPQCPPSGGCATRVLARMDKIDDI
                     CSIYPGPHPVVWCGDFARHAKTFARLYQLDIRTNC"
     gene            4357..>5795
                     /locus_tag="PKW60_07675"
     CDS             4357..>5795
                     /locus_tag="PKW60_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPJ99352.1"
                     /translation="MFAALLFFVSGATIQVEAPALAQPWDKAPAQFDITEFGPEAVAR
                     VLDPASGQEIPAKVQQQLDKRYVYWARTDDGSRPTLYTIETGVIPAAMPVFVGAGDML
                     SYGRYGVMADLGIGLWATAMPIDWDGDGDWDLLYSCQDKPQSGVYLYIQEAPNVFRLA
                     RRFTDGVWYCALADMNGDGEEDLLGGEHWWESVRDPEGWTKHNGPFKKPEGRIRAFLS
                     RQVDWDGDGLLDLIRAAGDWEDYGWDRAFDETGNWTRGPLHGYLWFHKNTGTNANPVY
                     AEGIRLEADDTPIDVYGNPCPCIADWDGDGDLDLLCGEFRDGFTYFENVGTRTHPRLA
                     RPRPAMTRNGLLKIDLCMMNPLPCDWIGDGRPDLIVGQEDGRVSVIINLGMSRGAPLF
                     AEEFFLKEIDPPIKSGGLVTPWLDTGTGVLYCGNTAGYIEQFRVRDGAYLEGQYLREG
                     RAPFRIMAGYNLSIQGPAEEKWGYTVPCLG"
BASE COUNT         1076 a         1711 c         1833 g         1175 t
ORIGIN      
        1 ggctgcaatg acgacggcat gatgtacgat tcgctcgatc agggcaaagg gcaactcagc
       61 gacggctggg gttacaacta tgtcggttac ctctgttatg accaggccac cggtacggag
      121 acctaccgcc cgcaggtcgt gcgtacgctc cgcaacctca tgaagccgtt gtacgagaat
      181 tacgcgtggg aaggcgaatc catcgacggc tatgccgatt ccatcgaagg cgcgctgtat
      241 ctcctgaacc gcgttcctgt ggcggaagga ttcacctggg tcgaccgcga gacaaagaac
      301 aatctggtcg atcatccgtc ccgcctcgag aacggcgaac tctggggcac catgaaactg
      361 cagtccaacg gggtgcgcac ggtgctgatg cactcgctca tgcacacgcg cggggtgatc
      421 gcccggccct ggcaacaggg tctcgaactc ggcgccgcgc cccgtggcga gggcctgtcg
      481 cttgtcgtgc gttctggcga agcctatcgc ggcgtgcttg aatttgacct gccccgttac
      541 cgcgtttatc ttggttttca aaaagactgg ccccgcatga acacccttcc cgaatggttc
      601 acggttgaac cggaacgcaa ttacacggtc acctgggtgg atacgggcgc gcgggcgatt
      661 tacaccggcc gccagttgca ttgcggcctg gcgatcgcgg tggaagccgg agaaaccctg
      721 cgcctcacgg tcgacccggc cccctgacgc gaaaaagccc cacgccggag gaccgttcga
      781 gaccggcgca gtacgcggca taaacaacaa cgcctggcgc aaggccaggc gttgtgcaat
      841 cggtatttcg cggagttcgg ctcagtattt cacgcccatg agatggcaga ggatgtagcg
      901 gtcaagctct tcgtaatccc gctccttgat gagctgcgcc tcgagcttgc ggtcgagtga
      961 gacgactttg tccagcagct tcttgaatgt attgaggctg ttggaaaggt gctttgccga
     1021 aacgttctgt ttctgcgtgc gcatgacctt gacatcgagt ccgacccatt cgccgttacg
     1081 gccgtagccg tactcctcaa gcacgcggac ctgattaaac gcccggcgga gattgtagga
     1141 gccgaacgat ttgtcctggt cgaacttgag tccgccctga tcgttgaggt gtacgctcca
     1201 gagtttgtcg tgcgcgaggg catagcccat gtcgtcggag gggtcgaggc ccgcgagaat
     1261 ggagtgagcc gtctcgacca gcacgcccat gcgcgccggg tcgcaggtca tatagcccag
     1321 acccacggca tggccaatgg tggagataat cgcgctgtct acgggttcgt tgggtttcgg
     1381 ctcaatcaga atccggagtt tggggtcgta ttccaggaag aggttgaagg cctcgacgat
     1441 ctgaagcacc tgcgtgcgcg gatttttcgc ctcgcggatg taggtgcctt cgcgcgcgag
     1501 ccacatcacc atgttctgcg cgtcgatcgc gttcttgatg tcgatggcga ccttcgtgcg
     1561 gtcaatggcg tacttgcgat cccgtggatt attgctggta tacgcaccgt cgatggtctg
     1621 tgttgcgaac cacagacgcg gcgcgacaaa ttcggctttc aacccctcac cgtcgagcgc
     1681 ccgcttgagc gcccgcgcct ccttcatgat ctggctgtgc gatttgttgt ccagatccgg
     1741 cacgacatca tcgtcatgaa actggatgcc gtgaaacccc atcgttttcg cggccttgaa
     1801 ctttttggcc atggcgatgg gcttgcgcac ttccggtccg aacgggtccg aaccttcatc
     1861 aatgttccac gggccaaacg agaaacggta cttgcctttg atcttcggca tcagcatctc
     1921 ctccgtccgc acggcgccgg gataccgcga catcgcccga gaaccgccgg ctccccatgc
     1981 gtgttacaca ccactgcaaa cacttcagca tacggcgcgc ataacgccgt ttgcaagcgc
     2041 cgaggcggcg cgggaatggg cgaatgccat cctttcgtcc tgcgcgaggt cagcagttgg
     2101 tacggatgtc gagctggtag aggcgggcga atgtctttgc gtggcgggcg aaatcaccgc
     2161 accagaccac cggatgcggg ccggggtaga tcgagcagat gtcgtcgatt ttgtccattc
     2221 gggcaagcac gcgggtggcg catccgccgc tgggcgggca ttgcggggaa tcgatgatat
     2281 cgccggccca ggcgtcgagg cggttttcgt cgcgatggta tttggccagc gtgatccgtt
     2341 cgccagcggg ccagttcaca tcgagtgcca gcgttttggg catctggtgg aagaagggcc
     2401 tcagcgtata ggacgtctct tttgcgccgg gcccgtgcag tctggtcgtg caggtgcaat
     2461 gcgaggcaaa gtagaggttt tcttcggtgt cgaattcggg attgtgctgg aaaccgcccc
     2521 ggccgaagag gtttgcgccc agcatcaggg tcagcgtggc gttcagatcg ttttcgcagc
     2581 cgcaggggac cagcgcgccg ttgttgatcg agaagctcag gcagggtttc cggtgtttca
     2641 ggaagaggca ctcgatcgtg atggcgtcgg catcgtattt ttccatgagc gcgagaaccg
     2701 cggcatgggc gcgcacggcg tccagaaatg ctttctgctc caggtccatg atgttgtccg
     2761 cccgcctgcg gatcctggcc gccagacgtt tcatttcggg cgtgatcgcc gtggcgtcgt
     2821 agagatcgtt gaaatagccc gcgggcacgc catgaacggg cagatcgaag aactcgacat
     2881 gatcctctgc ttctttttcg aggcgtcccg aaacccgcag caggcggctt tgagccatcc
     2941 gggccttggt atgaattgcg cgcaggccgc gttcgagagc gtcccagtgt tcgatggagt
     3001 ggatgtagtg caggcctttt cccgtgcgga aacgttcggg cggcagctgg tggttcgcgc
     3061 ccagggggtg gtagaggatg atcgggccgt cgtaggcgtc gagaatgggg acgatctttg
     3121 cgctgaacga gttccagaaa ttgaacagca gaagcgcatc gggtttgttc gcggtaatat
     3181 ccgcgatgaa tgcctggata tccgcgtcgg tgaacacggg ggcgcgctcc agctcgagct
     3241 ccacgctgag atcgcgtgtc atgtccgaga ggcgctgttc aaaggcagcc tgctgggcct
     3301 ccggctcgaa ctggttcccc ggccacgtga accactgatg tttgctccat gaatcctcgc
     3361 attttccttc gagcacgacc tccttcggag gatagaagaa ggcgccccgc acccgcgcgg
     3421 gtttcttgct gcgcggtgcg gcctgcagcg cggatttacc tgccgcgtgc gcctgcgaca
     3481 gcatccctga agccaccagg gcggccgagg agatcataaa cgtccggcga ttcacttcgt
     3541 agttcatgtg gtcaccttca cccggtaagt gcctgtcgct gagattggcg ccggggagtt
     3601 caacgccctg accccctgtc cagccgccag cggcgaatct ttgctcagcg cgtggccgtc
     3661 gttgccattg tggcccattc gcccgatcgg tgcaaccggc gatcctgtgt gggccatgtg
     3721 gaccatgtgg actgtgtggg cggggtgggc aaaggtggat ccccacgatt cctgtttgat
     3781 gcgcgagcta cgcggattct tctgacgtat ccgtgtgcag aaacaggaat gtccttcgca
     3841 cctgggcctc tgcagtttga cggcgggcgc tcacttagga gaggaatgaa tggcgtccga
     3901 cccgacactg ctggagttga agccgtcagc tgcaattgac ttcaaaactc cacccccaaa
     3961 tcggctatcc gattcacccc cgccagcggg ggacagcgcg ttgcatctga atctcttggg
     4021 ttagggcctg tgacgcctcg ccggaatggg agccgatgga gatccacgta agccgttgcc
     4081 tgctcggcat cttgtgtccc ccgctggcgg gggtggccgc gaagcggccg ggggtggatt
     4141 gattcagtgc aagcacgtgg gcctgcgcta cgcaagagaa gaacgcaggc aagatgcctg
     4201 cgctacgcgc tgcaaaaacc gggatgtgcc ggcaagggcg cagataattc ggcggtatcg
     4261 gccttaaaac gctgagagga ccagatccgg gttcttgttg caggcgctgg aaatgggtac
     4321 catgaccgcc ctgcaaggca tggaaggagt tcgcttatgt ttgctgcgct gttgttcttt
     4381 gtatcgggcg ccacgattca ggtggaggcg cccgcactcg cgcaaccctg ggacaaagcg
     4441 cccgcgcaat tcgatatcac ggaattcggc cccgaagccg tggcccgtgt gctggatccc
     4501 gccagcggcc aggaaatccc cgccaaggtg cagcagcagc tcgacaaacg ctatgtctac
     4561 tgggcacgta cggacgacgg gtcgcgtcca accctctaca ccattgaaac cggcgtgata
     4621 ccggccgcca tgcccgtgtt cgtcggagcg ggcgacatgc tgagttatgg ccggtatggg
     4681 gtcatggccg atctcggcat cggtctctgg gcgaccgcca tgcccatcga ttgggacggc
     4741 gacggtgact gggatctcct ctacagttgc caggacaaac ctcagagcgg cgtatatctt
     4801 tacattcagg aggcgccgaa cgtcttccgg ctcgcccggc gttttacgga tggcgtctgg
     4861 tactgcgcgc tggcggacat gaacggcgac ggcgaagaag acctgctcgg cggcgaacac
     4921 tggtgggaaa gtgtgcgcga tcccgagggc tggaccaaac acaacggccc gttcaagaaa
     4981 cccgaagggc gtatccgggc atttctgtcg cgccaggtag actgggacgg cgacggattg
     5041 ctcgatctca tccgtgccgc gggcgactgg gaggactacg gctgggaccg ggcgtttgac
     5101 gagaccggca actggacgcg cggcccgctc cacggctacc tctggtttca caaaaacacc
     5161 ggcacaaacg ccaatcccgt ttatgccgaa gggattcgcc tcgaagccga cgacaccccc
     5221 atcgatgtct acggcaaccc gtgcccatgc attgccgact gggacggcga tggcgacctt
     5281 gatctgcttt gcggcgagtt tcgcgacggg ttcacgtact ttgaaaatgt cggcacacgg
     5341 acccacccgc gcctcgcacg gccccgaccc gcgatgaccc gcaacggcct gctcaaaatt
     5401 gacctgtgca tgatgaatcc gttgccgtgc gactggattg gcgacggccg gcccgatctc
     5461 atcgtcggcc aggaagacgg gcgtgtcagc gtcattatca atctcgggat gagccgcggc
     5521 gcacccctgt ttgccgagga atttttcctg aaagagatcg atccgcccat caaatccggc
     5581 ggtctggtca cgccctggct cgataccggc accggcgtcc tctattgcgg caatacggcg
     5641 ggctacatcg agcaattccg tgtgcgtgac ggcgcctatc tcgaggggca atacctgcgc
     5701 gagggccgcg ccccgttccg catcatggcg ggctacaacc tcagcatcca gggtcccgct
     5761 gaagaaaaat ggggctatac agtgccctgc ctggg
//
LOCUS       DAOPBQ010000199         5758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12081_length_5758_cov_4.119937, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000199 DAOPBQ010000000
VERSION     DAOPBQ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12081_length_5758_cov_4.119937"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKW60_07680"
     CDS             complement(<1..592)
                     /locus_tag="PKW60_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPJ99353.1"
                     /translation="MARRDIRSLDRALFLFCMAVLCLTVAYPALRLFLVAAFNWAHEA
                     VVNNPGRAALINTALMSCATVVTSGLVGSGLAFALSRYAFPGRRVLAGLAYMPFTLPP
                     LVGVLSFYYLVGIDGLLPRFCEQRLGIGGLALRGPGAILLVHTYSFYVYFYAMAGAAL
                     ETVDVLQLEAARTLGAGRCRVFFRVMLPMLKPALLGA"
     gene            complement(632..991)
                     /locus_tag="PKW60_07685"
     CDS             complement(632..991)
                     /locus_tag="PKW60_07685"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPJ99354.1"
                     /translation="MRTNGDERRRYPRSRRAVPQIVPDDGPGILNHVDNISANGVLCH
                     TVQPIPLMTRMSIALDLPKPQNRRIECEGVVVRCEPHEVGDDRFKVAILYTKIDEDDQ
                     RAVEELVAREIEEAGIE"
     gene            1225..2142
                     /gene="metF"
                     /locus_tag="PKW60_07690"
     CDS             1225..2142
                     /gene="metF"
                     /locus_tag="PKW60_07690"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HPJ99355.1"
                     /translation="MNTSSKSAQLRDTYAHGRFGLSYEFFPPKTPEGEADLFENFDEL
                     AKYNASFVTCTYGAGGSTRDKTLAIASRLKREHAFPVASHLTCVGATRDALALFLEEA
                     GAAGIDYIVALRGDPPAGEKKFSPVPGGFRYANELVAFIRERFPGFGVAVGGYPETHP
                     EAPNRSTDLANLKRKVDAGADVVITQLFYDNDDFYRFRDRCTAIGIDVPIVPGILPVT
                     TAPQVKRLVALCGAHVPASLMERLDAHPDHSQGQFEVGVYHAIRQVEDLVGRGACPGV
                     HFYCLNKSRAVATILRTLALPNVAAFRTN"
     gene            2196..2432
                     /locus_tag="PKW60_07695"
     CDS             2196..2432
                     /locus_tag="PKW60_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HPJ99356.1"
                     /translation="MEKPKIAEKGPAVLELEPGTYWWCRCGHSATQPWCDGSHKSTSF
                     TPLEVKIEVKKRYALCQCKHSGNGAMCDGTHKRL"
     gene            complement(2499..2693)
                     /locus_tag="PKW60_07700"
     CDS             complement(2499..2693)
                     /locus_tag="PKW60_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99357.1"
                     /translation="MPTFDYHAIRAEGDEEKSFVGGVVVAKSRHEAKEKLRELGLKAT
                     MLKKARGIMAVLKWFEADIR"
     gene            complement(2796..3503)
                     /locus_tag="PKW60_07705"
     CDS             complement(2796..3503)
                     /locus_tag="PKW60_07705"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPJ99358.1"
                     /translation="MSRTRFQAVLFDFFGTLVDSLSASAHHDAVRAMGRRMNAPEEAF
                     LEQWLRDYDARGSGRWSTVEDCVLHTCEAIGVTPDRARAAEAARIRSSFIQQALVLRP
                     DARGTLEAIKARGLKTGLITDCPPELPELWPALGLEQLIDAPIFSCVVKLRKPDPRIY
                     QMACDRLGVVPGGCLFVGDGGSHELSGAETAGMTAVCIRAASEEGYDPHRVDSETWHG
                     PLVSSLSEILPFLGAGE"
     gene            3925..5211
                     /locus_tag="PKW60_07710"
     CDS             3925..5211
                     /locus_tag="PKW60_07710"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPJ99359.1"
                     /translation="MPYFPDIEALAREIQDWADLRHEQGGATAPVLARLRRALKQARQ
                     DLVRLPGARNLREAEPDELEAIRRLRPAGPRVLAKKLSDAEYRKRLRGAWLGRAAGCV
                     LGAPVENWTIDAMEGLAARNGTPFPPRDYWSDHPNPGEPCYLVSRIRDFLKGQIRAVP
                     ADDDLGYTLLGLLILEACGPEFTTEEVGDAWINYLPLACTAEHVALTNLKRGVSAQRA
                     GIVNNLYLEWIGADIRCDPWAYAAPGWPEKAAELAYRDAYLSHRRNGIYGSMFFAAAI
                     SAAFVLDDPLEALRLGLTEIPAKCRLARDIRWALRQAPALTGWREARALVDKRFQGMN
                     AVHTNNNACLTVFGLALGKQDFTRTIGITVAMGLDNDCTAATAGSLLGAILGIEGIPG
                     HWWKPFRNRVQSYLNGVPEFRNSDIVRRFEKLRIRP"
     gene            5357..>5758
                     /locus_tag="PKW60_07715"
     CDS             5357..>5758
                     /locus_tag="PKW60_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPJ99360.1"
                     /translation="MVLRFASIVCMVFAAFIAGAAETGVLVESDSGGVNALISSAPMQ
                     GTMNAIEAYLSQHEDLVEQPAGGEALSLSVRKCVDLALANNEKIFQAEKDIDAARARI
                     GQARSQMLPQLGASLTFVHTEFNERNPGGLFA"
BASE COUNT         1105 a         1806 c         1780 g         1067 t
ORIGIN      
        1 cagcgccgag cagcgcgggc ttgagcatgg gcagcatcac acggaaaaac acgcggcagc
       61 ggcccgctcc gagcgtgcgc gccgcctcaa gctgcaacac atcgacggtc tcgagcgccg
      121 cgccggccat ggcgtagaaa tacacgtaga acgagtaggt gtgcaccagc aggatggcgc
      181 ccgggccccg cagggccagt ccgccgatgc ccagacgctg ttcgcagaat cgcggcagga
      241 gcccgtcgat tcccaccagg tagtagaagc tcaacactcc gaccagcggc ggcagcgtga
      301 acggcatgta ggccagtcca gccagaacgc ggcgcccggg aaaggcgtag cgcgagagcg
      361 caaaggccag gcccgaaccc accagacccg acgtgacaac cgtagcgcac gacatcaacg
      421 ccgtattgat gagtgcagcc cggccggggt tgttcacaac cgcctcgtgt gcccagttga
      481 acgcggccac caggaagagg cgcagggcgg ggtaggctac ggtcaggcac agcacggcca
      541 tgcagaacaa aaataaggca cggtcaaggc tgcggatatc gcgccgggcc acctcaccct
      601 ccggactggc gccaacctgc cggcggggcc gttattcgat gccggcttcc tcgatttccc
      661 gggcgacgag ttcttcgaca gcgcgctggt cgtcctcgtc gattttggtg tacaggatag
      721 cgactttgaa gcggtcgtcg ccgacttcgt gcggttcaca ccgcacgacg accccttcgc
      781 attcgatgcg ccggttctgc ggcttgggga gatccagggc gatgctcatc ctggtcatca
      841 gcggaatagg ctgcacggtg tgacacagca cgccgttggc gctgatgttg tcgacatggt
      901 tgaggatacc ggggccgtcg tccggcacaa tctgcggcac ggcgcggcgg ctgcgcggat
      961 agcgtctccg ttcatctccg tttgttcgca tgagaccctc ttcagcgttc ctgcccccga
     1021 cgtgcccata gtatcctagc ggcctccgga atgcaacggg gataccgcgc tttttgccca
     1081 cagagctgac gcacggcacg gtctgctgaa atttgaattc aatcggcttg tcaaggcttg
     1141 atgaacccgg taccgggcgc taaaatgagc ccggatgact tcgcggtgcg cgatgtctga
     1201 gtccgggctg tgaaaaggaa cgtcttgaac acgtcatcaa aatcagcaca acttcgcgac
     1261 acctatgcgc atggccggtt tgggctgtcc tacgaattct tccctccgaa gacgcccgaa
     1321 ggggaagccg atcttttcga aaactttgat gaactggcca aatacaatgc atcgtttgtg
     1381 acctgcacct acggcgcggg cggcagcacg cgcgacaaaa cgctcgcgat cgcgagccgg
     1441 ctcaaacgcg aacacgcgtt cccggtcgcc agccacctca cctgcgtggg cgcgacgcgt
     1501 gacgccctgg ccctgtttct cgaagaggcc ggagccgccg gtatcgatta catcgtggcg
     1561 ttgcggggcg atccccctgc cggcgagaag aaattcagcc cggtccccgg cggatttcgc
     1621 tatgccaacg aactggtcgc atttatccgg gagcgttttc ccggtttcgg cgtggccgtt
     1681 gggggctatc cggaaaccca cccggaggcg cccaatcgca gcacggatct cgccaacctc
     1741 aaacgcaagg ttgatgccgg ggctgatgtg gtcattacgc agcttttcta cgacaacgac
     1801 gacttctacc gttttcgcga tcgctgcacc gccatcggca ttgacgttcc gatcgtgccg
     1861 ggcatcctgc ccgtgacgac ggcgccgcag gtcaagcgtc ttgtggccct gtgcggcgct
     1921 cacgtgccgg catcgctgat ggaacggctg gacgcccatc ccgaccactc tcagggccag
     1981 ttcgaggtgg gggtgtatca cgccatacgg caggtcgaag acctggtggg ccgcggcgcc
     2041 tgcccgggcg tgcactttta ttgcctcaac aaatcgcgcg cggtagccac gatcctccgc
     2101 acgcttgcgc tccccaacgt ggcggcgttt cgaaccaatt agcgtttccc tccccggatt
     2161 ctccgtggaa aggcagcaca tacaggagca tttaaatgga aaaaccgaaa atcgccgaaa
     2221 aaggccccgc ggtacttgag ctggagcccg gaacgtactg gtggtgccgg tgcgggcatt
     2281 ccgcgacgca gccctggtgc gacggctcgc acaagagcac gtcatttact ccgctcgagg
     2341 tgaagattga ggtcaagaaa cgctatgccc tctgccagtg caagcacagc ggcaatggcg
     2401 ccatgtgcga cgggacgcat aaacggctgt agatccggga gcttcggcca gccccctgca
     2461 aagactgtcc cagcgcggca gactggagag ggggacggct accggatgtc ggcctcaaac
     2521 catttcagga cggccatgat gccgcgcgcc ttcttgagca tcgtggcctt caggcccagc
     2581 tcacgcagtt tttcttttgc ttcatggcgc gatttggcga ccactacgcc tcccacgaag
     2641 gacttttcct cgtctccctc ggcccgaatg gcgtgatagt cgaacgtggg catgagcttc
     2701 ctccctgatc atcgatagac tcgccccgat tctagcacgt ttcgtccgga atggaaattt
     2761 cgcacgccct ggatggcgat cttcgtaagg ccgattcact cgccagcgcc gagaaagggg
     2821 agaatttcac tcagcgagct gaccagaggc ccgtgccagg tttcggagtc cacgcgatgg
     2881 ggatcgtagc cttcttcact ggcagcgcgg atgcagaccg cggtcattcc cgcggtttcg
     2941 gcgccagaga gctcgtggct gcccccgtcg ccgacaaaca ggcagccgcc gggcactacc
     3001 cccagccggt cgcaggccat ttgataaatg cgcgggtcgg gcttacgcaa tttcacgacg
     3061 caggagaaga tcggggcatc gatcagctgt tccagcccca gggccggcca gagctcggga
     3121 agctcaggcg gacaatcggt gatcaatccg gttttcaggc cgcgggcctt gatcgcctcg
     3181 agggtgccgc gcgcatcggg ccgcaatacg agggcctgct gaatgaacga agaccggatg
     3241 cgtgcggcct cggcggcgcg cgcccggtca ggcgttactc cgatggcttc gcatgtgtgg
     3301 agcacgcagt cttctaccgt ggaccagcgc ccggaaccgc gcgcgtcgta atcacggagc
     3361 cactgctcga ggaaggcctc ttccggtgcg ttcatacggc gccccatcgc cctgaccgcg
     3421 tcgtgatggg ctgaggcgct gaggctgtcc accagggtgc cgaaaaaatc gaacagcacc
     3481 gcctgaaacc gagtccggct catctggaaa gcggtttcgg cgcgcggccg tattgaggtt
     3541 gatcgcggag catgggtgcc ggatccttcg tcttgatttc tcttccccac tataaaacag
     3601 cgagagggac gtttccagca gcgcacattc taatacaaag cgcgcggtct ttgggtttgt
     3661 tgttctgttg atgagttctg agataaaagc tgctcgattc agccagcacg gatgaaaggt
     3721 gagagcccac ccgatgagag tccacccctg aatcggcgtc agccgattcg ggggtggact
     3781 ttttgtgctg aattgcaatc caccgcctca agttgcagca ctattatgct gaaccatttc
     3841 gtgctcccgg aaaattggcc agaagcacac ccctgagtgc atactcagtt tcttcatgca
     3901 acccgtgtct gggaggagta agggatgccg tattttccgg atattgaagc gctcgcgcgc
     3961 gagatacagg attgggccga tctgcggcac gaacaaggcg gagcgaccgc gccggtgctc
     4021 gcccggttgc ggcgcgcgct caagcaggcg cgtcaggacc tggtgcgtct ccccggggca
     4081 cgcaacctcc gggaagccga acccgacgaa ctcgaagcca tccgccggtt acgcccggcg
     4141 ggcccacggg tcctggcgaa gaaattgagc gatgctgagt accgaaagcg actgagaggc
     4201 gcgtggctgg gccgtgcggc gggctgcgtg ctcggtgcgc ccgtcgaaaa ctggacgata
     4261 gacgcgatgg aaggtctggc cgcgcggaat ggaacgccct tcccgccccg ggattactgg
     4321 tccgatcacc ccaaccccgg cgagccgtgt tatctcgtct cccggattcg cgattttctg
     4381 aaggggcaga tccgcgccgt gcccgcagac gacgatctcg gctatacgct tctggggctg
     4441 ttgatcctcg aagcctgcgg ccccgagttt acaacggagg aggtcggcga cgcctggatc
     4501 aactatctcc ccctggcctg cacggccgag cacgtcgcgc tgacgaatct caaacgggga
     4561 gtgtccgcgc aacgcgccgg gatcgtcaac aatctgtatc tcgaatggat cggcgcggat
     4621 atccggtgcg acccctgggc ctacgcagcg cccggctggc ccgagaaagc cgccgaactg
     4681 gcctatcgcg acgcgtacct ctcccaccgc cgcaacggca tctacgggtc gatgtttttc
     4741 gccgcggcca tttccgccgc cttcgtgctc gacgatccgc tcgaagcgct tcgccttgga
     4801 ttgaccgaga ttcctgcgaa atgccgcctc gcccgggaca tccgctgggc cctgcgccag
     4861 gcccccgccc ttacgggatg gcgcgaagca cgcgcactgg tagacaagcg cttccagggc
     4921 atgaacgccg tccataccaa caacaatgcc tgcctgacgg tattcgggct tgctctgggc
     4981 aagcaggatt tcactcgcac gatcggcatc accgtggcca tgggcctcga caacgactgc
     5041 accgcggcca cagcgggttc tctgctgggg gcgatcctgg gcatcgaggg cattcccgga
     5101 cactggtgga aaccatttcg aaaccgggtc caaagttatc tcaatggcgt tccggaattc
     5161 cggaattcgg acattgtccg gcgttttgag aagctccgca ttcggccatg aggttttagc
     5221 gcccggggat caattgaccc ccgctcagcg tgcgcgtatc atgcttcgca ggtacatgga
     5281 gttcatcaat gtgcgcccgg gacgacacgg acagggcgag agccgttcca cataatccaa
     5341 gaggatgagg ccagctatgg tattacgttt cgcttccatt gtgtgcatgg tcttcgccgc
     5401 gttcattgct ggcgcagcag agactggtgt tctggtcgag agcgattcgg gcggcgtgaa
     5461 cgccttgatc agcagcgcgc cgatgcaggg gaccatgaac gccatcgagg cgtatctgtc
     5521 gcaacacgag gaccttgtag agcagcctgc cggcggcgag gcgctctccc tgtccgtccg
     5581 gaaatgtgtt gatctggcac tggcaaacaa cgagaagatc ttccaggccg agaaggacat
     5641 tgatgcggcg cgcgcgcgga tcggccaggc ccgctctcag atgctccccc agctgggcgc
     5701 cagcctcacg tttgtccata ctgagtttaa cgagcgcaac cccgggggac ttttcgcc
//
LOCUS       DAOPBQ010000200         5739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12125_length_5739_cov_4.178395, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000200 DAOPBQ010000000
VERSION     DAOPBQ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12125_length_5739_cov_4.178395"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKW60_07720"
     rRNA            <1..336
                     /locus_tag="PKW60_07720"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            429..545
                     /gene="rrf"
                     /locus_tag="PKW60_07725"
     rRNA            429..545
                     /gene="rrf"
                     /locus_tag="PKW60_07725"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            994..2451
                     /locus_tag="PKW60_07730"
     CDS             994..2451
                     /locus_tag="PKW60_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99361.1"
                     /translation="MGAAEVPALPEGLEAATPTAESAPALPEGLDSGVSSGPALPEGL
                     GGEAPDGPALPDGLGETPSGPALPEGLGEAPDTEFLAGAEETPSKKLRPPWLHGFWDL
                     RGGVRTQNDPAQSKDATLGELRLQLEADKAWRGVVLKYRGDFIGDGVLEEGDFDLREL
                     SLMFSPARSVDVKVGRQVLTWGTGDLLFLNDLFPKDWQAFLSGRDVEYLKAPSDALRA
                     TWFTDWMNVDFVYTPQFDHDRFITGERMSFYNSLYGRTVGADRKVNFNAPSTWFVDDE
                     FALRLYRMVGSAELAFYAYSGYWKSPGGQRFPPMQATFPKLSVYGASLRGNFLKGIGN
                     VELAYFDSRQDRSGSNPFVNNSEFRLLLGYERELAKELTGGVQYYLEHMMDYSAYRNS
                     LISLIMNARDQDRHLFTLRLTKLLMRQDLTLSLFTFFSPSDVDAYFRPLVSYKVDDHW
                     TVECGANVFLGEKPSTFFGQFEDNTNVYGTVRYSF"
     gene            2605..3879
                     /locus_tag="PKW60_07735"
     CDS             2605..3879
                     /locus_tag="PKW60_07735"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPJ99362.1"
                     /translation="MSDKLAIDGGQKAVTNTLVSWPQFNDAAIQAVEAVLRSGKVNYW
                     TGKKGREFEDRFAQWQGSKYAISVNSGTSALHVGLAALGIGPGDEVIVPSYSFIASSF
                     SICQAGAIPRFADVNRDDHCISVESAEKLVNERTKAIMPVHLYGNVADMDAINAFAKK
                     HNLFVIEDNAEAFGGEYKGKKTGTLGDIAACSFCQNKTFTTGGEGGMVTTDNEDLAWE
                     ARSFRDHGYDVKARLSLLELEQKLPYIHNRVGWNYRMTEMQSVIGLAELDRMDNWNMP
                     ARRRNARIVMDALKDLPQVLYPPIDTDERRNGWFVMAFSLDIENMNCDIEQFVAAAGA
                     EGAPCWKVFWPQCHTEKAFTQKRGFGNSGFPFTSKEYATPSSVDFTTVDVPNAVWHQS
                     HTFTCFAFPTFTEDDMHQIANALVKVIKAYSK"
     gene            3988..4752
                     /gene="larB"
                     /locus_tag="PKW60_07740"
     CDS             3988..4752
                     /gene="larB"
                     /locus_tag="PKW60_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HPJ99363.1"
                     /translation="MEQKKLKELLEAVAGGSLTPDDAMERLKNLPFEDIGFAKLDHHR
                     HLRKGYPETVFAQNKTPEQVLAIVTRMREHGSNVLATRCSREVVEAVVAAHPDAVHHE
                     MARAITITVEAPLPRKGYAAVVSAGTSDLPVAEEAMVTCEALGTRVNRVYDVGVAGLH
                     RLLEQRPVLSGADVLVVCAGMEGALPSVVAGLVEQPVIAVPTSVGYGASFGGIAALLG
                     MLNSCATGISVVNIDNGFGAGVLAAMINRIAHGERD"
     gene            4792..>5739
                     /gene="larC"
                     /locus_tag="PKW60_07745"
     CDS             4792..>5739
                     /gene="larC"
                     /locus_tag="PKW60_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HPJ99364.1"
                     /translation="MKTLYFDCVCGAAGDMIAGALIDAGADFDALRAALDSLSIGGFT
                     VSVERVKKKGLEAAQFKVDVDPSAPQPHRHLRHVLEIVEGGKLPSSVVEASAETFRRI
                     AVCEAAVHGTTVERVHFHEVGAIDSIVDVVAAHFCLNLLGIERVESSRLPVGFGTVKA
                     AHGVMPVPAPATAKLLEGVPVWGGDLEGEWVTPTGAALISQLAQAYGPLPPMRIEQIG
                     YGSGQRDAPDRANVLRVLVGRSDGAEPGTETIAILETNIDDMNPELLPVLVEEALKAG
                     ALDAFLTPVVAKKGRAAHMLTVLSPENRVEVLVRTVLQNS"
BASE COUNT         1128 a         1552 c         1961 g         1098 t
ORIGIN      
        1 cggtacgcga gctgggttta gaacgtcgtg agacagttcg gtccctatcc tctgtgggcg
       61 taggagattt gagggaagct gcccctagta cgagaggacc ggggtggacg gacctatggt
      121 gctccggttg tcacgccagt ggcaccgccg ggtagctatg tccggtcggg ataagcgctg
      181 aaagcatcta agcacgaaac ccatcccaag ataagatctc cctggaccgc aaggtccctg
      241 aagggccctg gaagaccacc aggttgatag gctggaggtg tacgcatggc gacatgttaa
      301 gctgaccagt actaatcgcc cgtgaggctt ggcctttaat ctgccgaact caaatggggt
      361 ttggcaggac gcatcggtgg atttgatacc cacgttgcat ctaaccctta cattcctgtc
      421 aggttttcgc cggtgaccac ggcggtgggg ccacacctgt tcccattccg aacacagaag
      481 ttaagcccac ctgcgcctat ggtactgcgc gggcaactgc gtgggagagt cggtcgttgc
      541 cggcactatg attcgccccc gggattcgca agaatcccgg gggcgttctg tttttccccg
      601 ttcgctaggc ggcgcatccc gcgcatccac cgtccggatt ttgtaggggc ggccccctgt
      661 ggccgcccat ctcagcctca cgcgttgacc ttgatacagg gcaggcacag gggcctgccc
      721 ctacaagcgt gctacggggg tgtgcggccc gtgcgcacgg gttggactgc ggcggcaggg
      781 ggatggactc tcgtcatctc cactgagaac ttcgatggcg cgactcagag actggaaacg
      841 cgccccgttt ccgggtactc tgtacacagt ctcatcgttt tctggtgctg agtacgcagg
      901 tggagttcaa tgaccagtaa tttcaggcga cctgggagat ggctgtccag tttgaggata
      961 tgggcgactg ctctgctgct cacgtgggcg gccatggggg cagcagaagt tccagcgctt
     1021 cccgagggtc tggaggccgc cacgccgact gcggaatcgg cccccgcgtt acctgagggt
     1081 ctggacagcg gcgtatcgag cgggcccgcc ctgccggaag ggctgggtgg agaggcgccg
     1141 gatgggcctg cattgccgga tgggttgggc gagacgccga gcggccctgc gctgccggag
     1201 ggcctgggcg aggcgccaga cactgaattc cttgccggtg cggaagagac gccatccaaa
     1261 aaactgcgcc cgccctggct gcacgggttc tgggacctgc gcggcggcgt gcggacacag
     1321 aacgatccgg cccaatcaaa agacgcgacc ctgggcgagc tgcggcttca actggaagcc
     1381 gacaaggcct ggcggggggt ggtgctgaag taccgggggg atttcattgg ggatggcgtg
     1441 ctcgaggaag gcgattttga tctgcgcgag ctgtcgttga tgttttcacc ggcgcgctct
     1501 gtggatgtga aggtgggccg gcaggtgctg acctggggca cgggggatct gctgtttctc
     1561 aacgacctgt tcccgaagga ttggcaggcc tttctcagcg gccgggacgt ggaatatctg
     1621 aaggcgccgt cggacgcgct tcgcgcgacg tggttcacgg attggatgaa cgtggatttt
     1681 gtgtacacgc cgcagttcga tcacgaccga ttcatcaccg gggagcgcat gagtttttac
     1741 aattcgttat acgggcgcac ggtgggcgcg gaccggaaag taaatttcaa tgcgccgagc
     1801 acctggttcg tggacgacga gtttgcattg cgcctgtacc ggatggttgg ttcggcggag
     1861 ctggcatttt atgcctattc cggctactgg aagagcccgg gcggccagcg gtttccccca
     1921 atgcaggcga cctttccgaa actgagtgtg tatggcgcca gcctgcgggg caactttctt
     1981 aaaggcattg gaaacgtaga actggcgtac ttcgattcgc gacaggatcg gagtggttcc
     2041 aatccgttcg tgaacaacag cgaattccgg ctgctgctgg gctatgagcg cgaactggcc
     2101 aaggaactca cgggcggcgt gcagtattac ctcgagcaca tgatggacta cagcgcgtac
     2161 cgcaattcgc tcatctcgtt gatcatgaat gcccgggacc aggatcgcca cctgttcacg
     2221 ctgcggctga ccaaactgct catgcgccag gatctgaccc tctcgctgtt cacctttttc
     2281 agtccaagcg acgtagacgc gtatttccgc ccgctggtga gctacaaagt ggacgaccac
     2341 tggaccgtgg agtgcggggc caacgtcttt cttggcgaga aaccctcgac gttcttcggc
     2401 cagttcgaag acaatacgaa cgtgtacgga acggtgcggt attcgtttta accgggacgc
     2461 gctgttggcc ctgagagact gtttgcggcc gggagcggcc ttgatgcaga atcaatgcac
     2521 tggcgcgtaa cgggcttccc ttttgagcag gggtgttgtt caggcgggtt ggcttaggaa
     2581 taacaggcaa aggattacgt gatcatgagt gacaaattgg ccattgacgg cgggcagaag
     2641 gcggtaacca acacactggt ctcctggccg caattcaacg acgccgccat tcaagcggtg
     2701 gaagcggtgt tgcgctcagg aaaggtcaat tactggactg gcaaaaaagg gcgcgaattc
     2761 gaggaccgct tcgcccaatg gcagggcagc aaatacgcca tcagcgtgaa ctcggggacc
     2821 tcggcgctgc atgtgggact ggcggcactg ggaatcgggc cgggcgatga ggttatcgtg
     2881 cccagctatt cgttcatcgc atcgagcttt tccatctgcc aggctggtgc gatcccgcgg
     2941 tttgcggatg tgaaccggga cgaccactgc atcagcgtcg aatcggccga aaaactggtc
     3001 aatgagcgca ccaaagcaat catgcccgtg catctgtacg gcaatgttgc ggacatggac
     3061 gcgatcaacg cgtttgcgaa aaagcacaac ctctttgtaa tcgaagacaa tgccgaggca
     3121 ttcggcggag agtataaagg caagaaaaca gggacgctgg gcgatatcgc ggcgtgcagt
     3181 ttctgccaga acaaaacgtt cacgacgggc ggcgaaggcg gcatggtcac cacggacaac
     3241 gaggatctcg cctgggaagc acgcagtttc cgcgatcatg gctacgacgt gaaagcacgg
     3301 ttgagcctgc tcgaacttga acagaaactg ccgtatatcc acaaccgggt ggggtggaat
     3361 taccggatga ccgaaatgca atcggtcata gggctggccg agctggaccg gatggacaac
     3421 tggaatatgc cggcacgccg gcgcaacgca cgcattgtca tggacgcctt gaaagatctg
     3481 ccgcaggtgt tatacccccc gatcgacacg gacgagcggc ggaacggctg gttcgtgatg
     3541 gcattttcgc tggacatcga aaacatgaac tgcgacatcg agcagttcgt cgcggccgcg
     3601 ggggccgaag gggcgccctg ctggaaggtg ttctggccgc agtgccacac ggagaaggcg
     3661 ttcacgcaga agaggggctt tggcaattcg gggttcccgt tcacgtcgaa ggaatacgcg
     3721 acgccctcca gcgtcgattt caccacggtg gacgtgccca acgcggtgtg gcatcagagc
     3781 catacgttca cctgctttgc gtttccgact tttaccgagg acgacatgca tcagatcgcg
     3841 aatgcgctcg tgaaggtaat taaggcgtat tcgaaatagc cggcggcgat tttgaggcag
     3901 tggggcctgt gggtgccgag ggacccgcag gccccgcgat ccccttggag gcagcgtgcg
     3961 gcgcagaggc cctggagagc attcccgatg gagcagaaaa aattaaagga actgctggag
     4021 gcagtcgcgg gcggttcgct gacaccggac gacgcgatgg agcgcctgaa aaatctgccg
     4081 tttgaagaca tcgggtttgc aaagctggat catcaccggc atctccgaaa gggttacccg
     4141 gagacggtgt ttgcgcagaa caagacgcct gagcaggtgc tggccatcgt gacgcggatg
     4201 cgggaacacg gaagcaatgt gctggcgaca cgctgcagcc gggaagtggt cgaagcggtg
     4261 gtggcggcgc acccggacgc ggtgcaccac gagatggccc gtgcaatcac gattacggtc
     4321 gaagccccgc tgccgcgtaa ggggtacgcc gcggtcgtga gcgcggggac gtcggacctg
     4381 cctgtcgcgg aggaagcgat ggtaacctgc gaggccctgg gcacgcgcgt gaaccgcgtc
     4441 tatgacgtgg gcgtggccgg ccttcaccgg ctcctggaac aacggcctgt tttgagcggg
     4501 gcggatgtgc tggtggtgtg cgcggggatg gaaggtgcgc tgccgagcgt cgtggcgggt
     4561 ctggtggaac agccggtgat cgccgtgccc acgagcgtgg gatacggcgc gagctttggg
     4621 gggatcgcgg cactgctggg catgctgaat tcgtgcgcca cgggcatctc ggtcgtgaat
     4681 atcgacaacg ggttcggggc gggcgtcctg gctgcgatga ttaaccgcat cgcgcacggg
     4741 gaacgggatt agcgaaccgt cgcggcggcc ggggatctat aaggaacaga tatgaagacc
     4801 ctctattttg attgtgtctg cggcgcggca ggcgatatga tcgcgggtgc gctgattgac
     4861 gccggagcgg atttcgatgc cttgcgggcg gcgctggact cgctgtcgat tggcgggttt
     4921 acggtgtcgg tggagcgtgt gaagaagaaa gggctcgaag cggcgcaatt caaggtcgac
     4981 gtagacccgt cggcgccaca accgcaccgg cacctgcggc acgtgctgga gatcgtcgag
     5041 ggaggaaagc tgccctccag cgttgtggaa gcgagcgcgg agacgtttcg ccgcatcgcg
     5101 gtctgcgaag cggcagtgca cggcacgacc gttgagcggg tacattttca tgaagtgggg
     5161 gccatcgatt ccatcgtgga cgtggttgcg gcgcactttt gcctgaacct tctggggata
     5221 gaacgggtgg aatcgtcgcg gttgcccgtg gggttcggca cggtgaaggc ggcgcacggg
     5281 gtcatgccgg tgcccgcgcc cgcgacggca aaactcctgg aaggcgtgcc ggtctggggc
     5341 ggcgacctgg aaggagaatg ggtgacgccg accggcgcag cgctgatttc gcagctggcg
     5401 caggcctatg ggcccctccc gccgatgcgc atcgagcaga tcgggtacgg cagcggccag
     5461 cgcgacgcgc ccgaccgcgc caacgtgctg cgggtgcttg tgggccggag cgacggtgcg
     5521 gaaccgggca cagagaccat tgcgatcctc gagaccaaca tcgatgatat gaatccggag
     5581 ttgctaccgg tattggttga agaagcgttg aaagcaggcg cgctcgacgc atttctcacg
     5641 cccgttgtgg caaaaaaagg gcgcgcggcg catatgttga cggttttgtc gcctgaaaac
     5701 cgggtggagg ttcttgtgcg gaccgtgttg cagaactcc
//
LOCUS       DAOPBQ010000201         5673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12293_length_5673_cov_3.882164, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000201 DAOPBQ010000000
VERSION     DAOPBQ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12293_length_5673_cov_3.882164"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1434
                     /locus_tag="PKW60_07750"
     CDS             91..1434
                     /locus_tag="PKW60_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPJ99365.1"
                     /translation="MKPTRRDMLKVLAGSAALVEGGYAMRTAQAADEASPAPQGPVPT
                     PEPRIERFERLAYGMFIHWGIYSQLGKGAWVMNQEKIPLDEYKRLSDTFTASEFDGRA
                     IARIARRAGQRYATLTTRHHDGFSLYDTRGLSDFDAVQSPAKRDLVAEFIEGCRAEGI
                     VPMCYHTTLDWYHPDFNANFDAYLDYLHASVELLCTQYGELGGFWFDGNWSKRDVDWK
                     EDRLYALIRKHQPQAMIINNTGIHELGRLGHPEIDCATFEQGRPTPMDRRGMSKYISA
                     EMCQTMNTHWAISTMDYNYMSPADIIVNLCACRKVGANYLLNVGPTAAGAVPGYEQAT
                     LERASQWLALHGEPIYDGKPCGVSGPGVDFGLDVDGTLYLFVHDLRPGHGRQSSEVVG
                     AGPRAFEGVGQPVTSVTWMDNGEELKFEQNAQLLTVNATAFPHGTNTVVRVARVS"
     gene            1708..3456
                     /locus_tag="PKW60_07755"
     CDS             1708..3456
                     /locus_tag="PKW60_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HPJ99366.1"
                     /translation="MAQEDRRKRSELSMNPQIERDRQMIVEARQKGKGALTGVFVRLS
                     GPGWLQSAITLGGGSLSSSMYLGLLGGFAFLWLQPLAMVAGIIMLSAISYVTLSTDET
                     PLKAINRHVNPVLGWGWLLASMTANIVWSIPQYSLGIASLRQNLFEGLLGENGALGGA
                     GLYVAAAIVLCIAMSASMLYSIGGKGAKIFDILVKCLVGTTVACFVAVVVRLLMTGQI
                     QFGEILSGFTFHTQLLTQPVSAFAEQIAHVAPAYQGYWSSMIVGQQRDVMISAGATAV
                     GINMTFLLPYSMLRKGWDREFRGLAIFDLSTGLFFPFLIATSCVVIAAASQFHGVAAP
                     GLLGETDSQGTPIQPAANLVGPYAELLAGRVKAEIGEEACAALTPEELEAQKAALPES
                     DRTIAAMIVRRDAFNLAAALAPITGDKFARYVFGLGVMGMATGAATMLTLINGLCFCA
                     LLDVPARGWQQRVGMFMPAIAIVVPLFWKGAYMWLAVPTSVFCMTLLPFAYISFFLLM
                     NQRKYLGADTPMGAKRIVWNILMVFSCLLACVGAFWSIYSRFGGLKTALIIGGFLVLV
                     AIGHAFRDSRHREVTA"
     gene            complement(3482..4630)
                     /locus_tag="PKW60_07760"
     CDS             complement(3482..4630)
                     /locus_tag="PKW60_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPJ99367.1"
                     /translation="MESDDSDIARDASESLNALDAMRAIWRNDAVGTWQWDLHTGKAL
                     WNTRMFELLGLDPTVDTISGAMLLDTVVPEDRPRIESAIAECIRTAKPFEGEFQIVRP
                     DGEMRWLFARGAGMHGEPGAPSELLGVVIDITERKRDEIEVQRATRNLEVRLGEDSET
                     STQQPRELRRLAAQLTQAELAERKRIAGLLHDTVQQTLVAAILGAEKARRQISNAGAR
                     DTLLKVITAIQEVVARTRLLSIQLDPPVLAQSTLSESLRWLAASKREHYGLQVTFQES
                     AEPAAPSEEVKFFLFEAARELLFNVVKHGGVLAASMSLTAQDGALCLSIDDEGQGCNL
                     DLLGDASGEHGLGLAMIRDRAAMLGGEVSLSSAPDAGFHVRIRVPLDD"
     gene            5077..>5673
                     /locus_tag="PKW60_07765"
     CDS             5077..>5673
                     /locus_tag="PKW60_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPJ99368.1"
                     /translation="MSYTVCLIPGDGIGPEVSEAARRVIDAANAHINWVELSAGAGAV
                     AEHGDVLPDSTVQAIEIHRVALKGPITTPIGKGFRSVNVQLRKRLNLYAAVRPVRSMP
                     GITTRYENVEIVVIRENTEGLYSGLENEITTGVVQSLKIATRTACERIARFAFHYARE
                     RGRRRVTVFHKANIMKLSDGLFLDCAREVHEKEAPDIQY"
BASE COUNT         1065 a         1791 c         1740 g         1077 t
ORIGIN      
        1 atatgatctg cgcgcggtgg tgggtgaggc aatgccgcct gatttcggat tcggcgttat
       61 ggtttggctc aaaaaaagga tgacttcacc atgaaaccca cacgccggga catgctcaag
      121 gttcttgcgg gcagcgctgc gctggtcgag ggcggttacg ccatgcgcac tgcacaagcg
      181 gccgatgagg cctcgccggc accgcagggg cctgtcccca cgcccgaacc gcgcatcgag
      241 cggtttgaac ggctggctta cggcatgttc atccactggg gcatctattc tcagctgggc
      301 aagggcgcct gggtcatgaa ccaggagaaa atccccctcg atgagtacaa gcgtctgagc
      361 gacaccttca cggcctcgga atttgacggc cgggccattg cacgcatcgc ccggcgcgcg
      421 ggacagcgct acgccacgct taccacccgc caccacgatg gattcagcct ttacgacacc
      481 cgggggctct ccgatttcga cgccgtgcag agcccggcga aacgcgacct cgtagcagag
      541 ttcatcgaag gatgccgcgc cgaaggcatc gttcccatgt gctaccacac cacactggac
      601 tggtaccacc ccgatttcaa cgccaatttt gacgcgtacc tcgactacct gcacgcctca
      661 gtcgagctgc tgtgcactca atatggcgaa ctgggcggat tctggtttga tgggaactgg
      721 agcaagcggg atgtcgactg gaaggaagat cggctgtacg cgctgatccg aaaacaccag
      781 ccccaggcca tgatcatcaa caatacgggc atacatgagc tgggccggct cggccatccc
      841 gagattgact gcgccacgtt cgaacagggc cgccccactc ccatggaccg ccgcggcatg
      901 tccaaataca tcagcgccga gatgtgccag accatgaaca cgcactgggc tatttccacg
      961 atggattaca actacatgtc gcccgcggat atcatcgtga acctgtgcgc ctgccgaaaa
     1021 gtcggtgcga actacctgct caatgtcggg cccaccgcgg ccggcgccgt tccaggctat
     1081 gagcaggcca ctctcgaacg cgccagccag tggctggcgc tccatggcga gcccatctat
     1141 gacggcaaac cctgcggcgt ttcaggcccg ggggtggatt tcggcctgga cgtggacggc
     1201 acgctttacc tcttcgtgca cgacctccgg ccgggacatg gccggcagtc gagcgaggtc
     1261 gtgggcgccg gcccgcgcgc attcgaaggt gtcgggcagc cggtgacgtc ggtaacgtgg
     1321 atggacaatg gcgaagagct gaaatttgag cagaacgctc aactattgac cgttaatgcc
     1381 accgcatttc ctcatgggac taacacggtg gttcgcgttg cgcgggtatc ctgaggatca
     1441 ggagttcacc cgaaaccagg cccaatataa agggggaaaa cgccgaaggt ttgaaaagca
     1501 ggctgcattc cccgcatgat gcgttatgcg cacgcgggga acaggtgcat gggacgccat
     1561 cgaagaccag cgttgacgcg caggaaccgc ctgctgtccg caggccacgc gggcgcccac
     1621 gcatacgtat gaggccatac cggccccgca ggagggccag ccgcggcgtt cgcacccgaa
     1681 aagactgaag agaggagact gcattccatg gctcaggaag acagacgtaa acggagcgaa
     1741 ctttccatga atccgcagat cgagcgcgat cggcagatga tcgtggaagc ccgccagaaa
     1801 ggcaaaggcg ctctgacggg cgtatttgtc cggttgtcgg gaccggggtg gcttcagagc
     1861 gcgatcacac tgggcggagg ttccctgtcg tccagcatgt acctgggcct tctgggcgga
     1921 ttcgccttcc tgtggctgca acccctggcc atggtggccg gcatcatcat gctgagcgcc
     1981 atttcctacg taacgctgag caccgacgaa acgccgctga aagccatcaa ccgccacgtc
     2041 aacccggtgc tgggctgggg ctggctcctg gcctccatga ccgccaacat cgtctggtcg
     2101 ataccccagt acagcctggg cattgcctcg ctgcgccaga acctcttcga aggccttctg
     2161 ggtgaaaacg gcgctctggg cggggccggc ctgtacgtgg ccgcggccat cgtcctctgc
     2221 atcgccatgt cggccagcat gctctacagc atcggcggca aaggcgccaa gatttttgac
     2281 atcctggtga aatgcctcgt gggcacgacg gtcgcctgtt tcgtcgccgt ggtcgtcagg
     2341 ctgctgatga ccggccagat ccagttcggc gaaatcctga gcggattcac gttccacacg
     2401 cagctgctca ctcaaccggt ctcagcgttt gcggaacaga ttgcccacgt cgcccccgcc
     2461 tatcagggct actggtcgag catgatcgtc ggccagcagc gcgacgtcat gattagcgcg
     2521 ggcgcgaccg ccgtgggcat caacatgacc ttcctgctcc catattcgat gctgcggaaa
     2581 ggctgggatc gcgagttccg cggactggcc atctttgacc tgtctacggg cctgttcttc
     2641 cccttcctga tcgccacgag ctgcgtagtc atcgcggcgg ccagccagtt tcacggtgtg
     2701 gcggcgccgg gcctgcttgg cgagaccgac agccagggaa cgcccattca acccgcggcc
     2761 aatctggtcg gtccctacgc cgaattgctg gccgggcgcg tgaaggcgga gatcggcgag
     2821 gaagcctgcg cggcgctcac cccggaagaa cttgaggccc aaaaggcggc cctgccggag
     2881 tccgaccgca cgatcgcggc catgattgtg cgccgcgacg cattcaatct tgcggcggcc
     2941 cttgcgccca tcacgggcga caaatttgcc cggtatgttt ttggactggg cgttatgggc
     3001 atggctacgg gcgcggccac gatgctcacg ttgatcaatg gtctgtgctt ctgcgccctg
     3061 ctcgatgtgc ccgcgcgggg ctggcaacag cgcgtgggca tgttcatgcc cgcgatcgcc
     3121 attgtggtgc cgctgttctg gaaaggcgcg tacatgtggc tggcggttcc gacgtcggtg
     3181 ttctgcatga ccttgctgcc gtttgcctac atttcgtttt tcctgctcat gaaccagcgg
     3241 aagtaccttg gcgccgacac tccgatgggc gccaaacgga tcgtctggaa cattctcatg
     3301 gtgttttcgt gcctgctggc ctgtgtgggc gctttctgga gcatctattc gcgctttgga
     3361 gggctcaaga ctgccctgat catcggcggg tttctcgtac tggtcgctat tgggcatgcg
     3421 ttccgcgact cccggcatcg cgaggtcacg gcctgacgct gccgccctta aacgcggccg
     3481 gtcagtcgtc cagaggaacc cggatacgca cgtgaaatcc tgcgtcaggc gcggacgaca
     3541 gcgatacttc gccgcccagc atggcggcac ggtcccgaat catggcaagt ccgaggccat
     3601 gttcaccgga ggcgtctccg agcaggtcga ggttgcagcc ctggccctcg tcgtcgatcg
     3661 agaggcagag cgccccgtcc tgggcggtca ggctcatgga ggccgccagc actccgccgt
     3721 gcttgaccac gttaaagaga agctcccgtg cggcttcaaa caggaaaaac tttacttctt
     3781 cggacggggc ggcgggttcc gccgattcct ggaaggtgac ctgtaatcca tagtgttccc
     3841 gtttgctcgc cgcgagccag cgcagcgatt ccgacagggt gctctgggcc aggaccgggg
     3901 gatcgagctg aatcgacagc aggcgcgtcc gggcaacgac ctcctgaatg gcggtaatga
     3961 ctttcagcag cgtatcccgg gcgccggcgt tgctgatctg gcgccgggcc ttctccgccc
     4021 ccagaatcgc cgcgaccagg gtctgttgca cggtgtcgtg cagcaaaccg gctatacgtt
     4081 tccgttcggc gagttcagcc tgcgtgagct gcgcggcaag ccgcctcagc tcccgcggct
     4141 gttgcgtgct ggtctccgaa tcctccccga gccggacttc gaggttccgg gtggctcgct
     4201 gcacttcaat ttcatcgcgt ttacgctcgg tgatgtcgat gaccacccca agcagttcgg
     4261 atggggcgcc cggttctccg tgcatgcctg cgccgcgagc gaaaagccac cgcatctctc
     4321 cgtctggacg cacaatctga aattccccct cgaaaggctt tgccgtgcgg atgcattccg
     4381 cgatagccga ttcgatacgc gggcgatctt ccggcacgac cgtgtcaaga agcatcgcgc
     4441 cggagatcgt gtctaccgtg ggatccaggc caagcagctc gaacatgcgc gtgttccaca
     4501 acgcttttcc ggtgtggaga tcccattgcc acgtccccac tgcgtcgttc cgccagatcg
     4561 cgcgcatggc gtccagcgca ttcagggatt cgctggcatc gcgtgctatg tcgctatcgt
     4621 cgctttccat gatggcctat tgcccctgcc aagcctgcca gccggcatcc agctcgcgga
     4681 tgaggccgcc gcggcacttg caccggagcc tcgcgacgcg catggccggc atggtgggcc
     4741 gggtgcccca ggatctttcc cacaggtgcc tgcatgccct caccggagcc attttatcgc
     4801 gaaaacagcc gcgaatcgag aaatcccgcg ccaggatcgc tgaaatttgc gaaaaaggcg
     4861 tctgggtctg gtcatttact gattttcccg cgtaatggcg acttttccta tcgggatggc
     4921 ataaaatgtg ccccagagga agccggtcat cccgagcgcc cacggccgca cttgccaggt
     4981 cacgatgcgg gctggggatg gatggcggcg gcttcccgta aagcaccatt cggttcaagc
     5041 cagaagccgg ctgcacatta tatgagagga agctccatgt cgtacacggt atgtctgatc
     5101 cctggggatg gaatcggccc cgaagtatcg gaagccgcgc gccgcgttat cgacgccgcg
     5161 aatgcacaca tcaattgggt ggaattgtcc gccggggcgg gagcggtcgc cgagcacggc
     5221 gatgtgttgc ctgactcgac cgtgcaggcc atcgagatcc atcgcgtggc cttgaaaggc
     5281 cccatcacca ccccgatcgg caaggggttc cggagcgtga acgtccagtt gcgcaaacgg
     5341 ctcaatctgt atgccgccgt gcgtccagtg cgcagcatgc cgggcatcac gacgcgctac
     5401 gaaaacgtcg aaatcgtcgt gatccgcgaa aacaccgagg ggctctattc aggactcgaa
     5461 aacgaaatca cgaccggcgt ggtgcagagc ctcaaaatcg ccacccggac cgcgtgcgaa
     5521 cgcatcgccc ggttcgcgtt ccattacgcc cgcgaacgcg gccggcgccg cgtgaccgtc
     5581 ttccacaagg ccaacatcat gaaattgtcc gacgggctct tcctggattg cgcccgcgag
     5641 gtccacgaaa aagaagcccc ggacattcaa tac
//
LOCUS       DAOPBQ010000202         5672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12296_length_5672_cov_4.243724, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000202 DAOPBQ010000000
VERSION     DAOPBQ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12296_length_5672_cov_4.243724"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PKW60_07770"
     CDS             complement(<1..864)
                     /locus_tag="PKW60_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPJ99369.1"
                     /translation="MTHFLAFDGGGTATRAGLYDLQGRLLAEAVGGASNPVALGEERC
                     LEVLTQVGNEALAHANGGLEAVGAALSGTWSSGMGFWLADRLRERFEAARVIVCDDIR
                     PILFANIGDSPGILATAGTGSSVVAQRSDGRSDCVGGRGALFGDDGSAFQIGQSAMRA
                     AARAQDGLGPDTILVALLPEAAGVESFRMLPLWAGRASMRDVAGLAKAVAQAADSDMV
                     ARSIITDQAHRMAELVRAGAVKAGLPPDTPVLLNGGVFEQCPLYLDAFRAHLMASGMR
                     GEAFLAPVRGHR"
     gene            complement(861..2165)
                     /locus_tag="PKW60_07775"
     CDS             complement(861..2165)
                     /locus_tag="PKW60_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HPJ99370.1"
                     /translation="MKPFPSSFLWGAAVSAHAVEGGHFDSDWWRWEQRSGRIADGTTS
                     QAGCDHRARFREDIALARKLGLNALLISLEWSRIEPREGDFSSEAMAHYAEVLRAMAG
                     EGLEPVVALQETTVPDWFASSGGWLRSDAAKLFSRYAEQAGAALGRLCRYWVPILTPV
                     ATVRMGYLQGLWPPGRRSLLAGWKALQHMAHGHAAAYHAMREHSPEAQIGASVLGELV
                     RPADQTRPWDLRVARWEQALSNEVWPAALISGRWPRPFGEERALAGTVDFVGVSYYGS
                     RRVRFRPGRGAYAQLVNGQGKRVPPHDCEPDAEGLAAVLHAAAGFGKPLMVLGNGLAT
                     DDDRARSHYLLDHVAALHSVVDSGVEVLGYMHRSLLDGFEWDRGYRARYGLVHVSGGT
                     MARTPNTSAYLYRDIASAGEIRKGTLARYCPDWEPPGGLELP"
     gene            2451..3536
                     /locus_tag="PKW60_07780"
     CDS             2451..3536
                     /locus_tag="PKW60_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99371.1"
                     /translation="MQRDFRGALDGGHSLSLLEDRTVSQRIAFVGFRHAHVYGLYHLA
                     RTHADLEVVAVCEEDAETRAQLKELGIAVTHERYTTMLDEVPCDIVACADYFAVHAER
                     VTDALEREKHVLCDKPICVHLEELNRIESLAREKDRRVGCILDLVDGAPFVTLRDIIL
                     RGDIGDVLSITILGQHPLNYGTRPMWFFEEGSHGGTINDIGVHVIDALPWLTGQPVSE
                     IVAARVWNGRIRQHPHFEDGAMLMLRLANGCGVMADLSYFSPDAGGYDLPTYWRFTVT
                     GTKGAAEAGVNLNKVIVWPDAEGGPREIPLLAPRTGGYLEDFLADIAGRPAPGGLDTA
                     RVFRSARISLLAQRAATVGPIRLKVGA"
     gene            complement(3544..4902)
                     /locus_tag="PKW60_07785"
     CDS             complement(3544..4902)
                     /locus_tag="PKW60_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009101016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99372.1"
                     /translation="MISRRAFLKTAAASAPLILSTRVLGMDGPGPNETVNVGLIGLGG
                     RCQDIAGTCLHIPEIRITAVCDCFKPRVDACLNGAGKDRGWTGYTDFREMIEKENLDG
                     VMVETTTHARAWVACHAMAMGMDVYIEKPMCLTIAEGREMVLLARKYQRVTQVGTQQR
                     TIALNNWASDLVKNGAIGRVTEVLAPNFVGPLRWEPKPAEPMPEGGSDNWWDLWLNQA
                     EFRPYHSELHRQWAHWWDYDGGGVSFGVTGWGTHSYDQIQRGLGTDETGPTEICLEEA
                     VQTLPSGAFEERQPSPEETGAAYYSMARFTGPRAKMRMKYANGTVLNLSLDADWGPGL
                     GCIFIGEKGRIEINRDKISANPRELLDAPGNPGHLQVPETKPHIEDWVRCIKTRERCT
                     ADIEYGQRSSSLCYLVNIARDVGRVGDTLYWNPEAERFTNCDEGNAMLSRPRRPGYEL
                     PA"
     gene            5229..5311
                     /locus_tag="PKW60_07790"
     tRNA            5229..5311
                     /locus_tag="PKW60_07790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5261..5263,aa:Ser,seq:tga)
     gene            complement(5503..5578)
                     /locus_tag="PKW60_07795"
     tRNA            complement(5503..5578)
                     /locus_tag="PKW60_07795"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5543..5545),aa:Lys,seq:ctt)
BASE COUNT         1042 a         1959 c         1686 g          985 t
ORIGIN      
        1 ccggtgcccc cgcacgggcg ccaggaacgc ttcccctcgc atgcctgaag ccatgagatg
       61 cgcgcgaaac gcgtccagat agagcgggca ctgctcaaat acccccccat tgagcaaaac
      121 cggggtgtcg ggaggcaggc cggcttttac ggcccctgcc cgaaccagtt ccgccatacg
      181 atgggcctga tcggtgatga tgcttctggc aaccatatct gaatcagcgg cctgcgccac
      241 tgctttcgcc agcccggcaa cgtcccgcat ggacgccctc ccggcccaca gcggcaacat
      301 gcggaacgac tccacaccgg ccgcttccgg cagcaatgcc acgagaattg tgtcgggccc
      361 aaggccatcc tgcgcccggg cagcggcccg catggcgctc tgcccgatct ggaacgcgct
      421 cccatcgtct ccgaacagcg caccgcgccc tccgacacaa tccgaccgtc cgtcggacct
      481 ttgcgccacg acgctcgaac ccgtccccgc ggttgccaga atgccgggcg aatcgccaat
      541 atttgcaaag agaataggcc ggatatcgtc gcacacgatg acccgcgccg cctcaaatcg
      601 ttcgcgcagg cggtccgcca gccagaaacc catgccggag ctccaggttc cagacaaagc
      661 cgcgcctacg gcttccaggc cgccattcgc atgcgccagg gcctcgttcc caacctgtgt
      721 caagacctcc aggcaccgtt cttcgcccag agccacggga tttgatgccc cgccgactgc
      781 ttccgcgaga agccgcccct gcaggtcgta gagtcccgcg cgggtggccg tgccgccgcc
      841 gtcaaaggcc agaaaatgcg tcatggcagc tccaatccgc ccggaggctc ccaatccgga
      901 cagtaccgtg ccagcgttcc tttgcgaatc tcaccggccg atgcgatgtc cctgtacagg
      961 tacgccgagg tattgggcgt gcgtgccata gtgccgccgc tgacgtgcac caggccatag
     1021 cgcgcacggt aacctcgatc ccattcaaag ccgtccagca gagagcggtg catataaccg
     1081 agcacctcga cgcccgaatc cacgacggag tgcagcgccg cgacatggtc cagtagataa
     1141 tggctgcggg cacggtcgtc gtcggtggcc agaccgtttc ccagcaccat cagcggcttg
     1201 ccgaagccag ccgcggcgtg caagacagcc gccaaaccct ccgcatctgg ctcgcaatcg
     1261 tggggcggta cgcgtttgcc ctgcccgttc accagctgtg cataagcgcc ccggcccggg
     1321 cgaaaccgga cccgccgtga accataatac gacactccca caaaatcgac cgtgccggcc
     1381 agcgcccgtt cctctccgaa tggccgcggc cagcggcctg agatcagcgc cgccggccaa
     1441 acctcgttgg acaaggcctg ctcccagcgc gccacgcgga ggtcccaggg gcgcgtttgg
     1501 tcggctgggc gcaccagctc gcccagcaca ctggcgccga tctgtgcctc cggagaatgt
     1561 tcccgcatag cgtggtatgc cgcggcatgt ccgtgagcca tgtgctgcaa cgctttccac
     1621 cccgccagaa gactccggcg ccccggaggc cagaggcctt gaagataccc catccggacc
     1681 gtcgccaccg gcgtcaatat gggcacccag taccggcaca gccttccgag cgccgccccc
     1741 gcctgttccg cgtaccggga gaagagtttc gccgcatcac tgcgcagcca tcctccgctc
     1801 gaggcaaacc agtccggcac ggtcgtctcc tgcagcgcga caacgggttc cagcccttct
     1861 ccggccatcg cacgcaagac ttccgcgtaa tgggccatgg cctcggacga aaaatcgccc
     1921 tcacgcggct caatacggct ccattccagc gatatcaaca acgcgttgag gcccagtttg
     1981 cgcgccagcg cgatgtcctc acgaaaccgg gccctgtgat cgcagccggc ctgagacgtt
     2041 gtcccgtcgg cgatgcggcc cgagcgctgt tcccatcgcc accagtcgct gtcaaaatgg
     2101 cccccttcaa cggcatgcgc cgatacagcc gctccccata aaaaactcga cggaaaaggt
     2161 ttcacaactc cctgctttcc cggtgcaatg cgccacgcag ttccggaggt cgatggcgtc
     2221 gttcgcgcgc tgtccggcgg gtccccatga ccccggcggc tccagtatgc gaatttgtcc
     2281 cgcttacgct caaccctgcc cgcatgacgg cccggccctg aaaaagaagg cgcttctcgc
     2341 ctgctgcgcg cccggcggcg attggggcac atgccctacg ttgcgtttgc gggagccggg
     2401 cgggtaaagt gcatgcacag ggcaaaggcg cctgtgggga gagggggcgc atgcagcggg
     2461 atttccgcgg cgcccttgac ggcggccaca gcttgtcgtt gctggaggat cgtaccgtga
     2521 gtcaacgaat agcttttgtc ggatttcgtc atgcgcatgt ctacggcctg taccatctcg
     2581 cccggaccca cgcagatctt gaggtcgtcg cagtctgtga agaagacgcc gaaacccggg
     2641 cccagctcaa ggagctgggg atcgccgtaa cacacgagcg ctacacaacg atgctcgacg
     2701 aagtgccctg cgacattgtc gcctgcgccg actatttcgc cgttcacgcg gaacgggtga
     2761 ccgatgcgct cgaacgtgag aaacacgtct tgtgcgacaa gccgatctgt gttcatctgg
     2821 aagagctgaa ccgtatcgag tcgctggcgc gtgaaaaaga ccgccgcgtc ggctgcatcc
     2881 tcgacctggt cgacggcgcg ccgtttgtga ccctgcgcga cattatcctt cgtggcgaca
     2941 tcggcgacgt gctcagcatc acgatcctcg ggcagcatcc cctgaactac ggtacgcgcc
     3001 cgatgtggtt ttttgaagaa ggctcgcacg gaggcacgat caacgatatc ggtgtacacg
     3061 tgatcgatgc gttgccgtgg ctgaccggcc agcccgtctc ggagattgtg gcggcccgcg
     3121 tatggaacgg ccgcatcaga cagcatcccc acttcgaaga cggcgcgatg ctcatgctga
     3181 gactggcgaa cggctgcggc gtcatggccg acctctcgta tttcagtccc gacgccggcg
     3241 gctatgacct gcccacgtac tggcgattca ccgtaacagg cacaaaaggc gccgcggaag
     3301 caggcgtcaa tctcaacaaa gtcatcgtat ggccggatgc cgaaggcgga ccgcgcgaga
     3361 tcccgctgct tgccccgcgg accggcggct acctggaaga ttttcttgcg gacatcgcgg
     3421 gccggcccgc accgggcgga ctcgacacgg cacgggtgtt tcgcagcgcc cggatcagcc
     3481 tcctggcaca acgcgcggcc accgtcggac ccattcgcct gaaagtcggc gcgtgaaacg
     3541 cgctcaggcg ggaagctcgt aacccggacg gcgcggacgg ctcagcatcg cgttgccttc
     3601 gtcgcagttg gtgaaacgtt ccgcctccgg attccagtac agcgtgtcgc cgacgcggcc
     3661 cacgtcgcgg gcgatgttga ccaggtagca aagcgacgaa ctgcgctgac cgtattcaat
     3721 atcggccgtg caccgctctc gtgtcttgat gcagcggacc caatcctcaa tgtgcggctt
     3781 cgtctccggc acttgcagat ggccgggatt gccgggtgcg tcgagcagtt ctctcgggtt
     3841 ggcggagatc ttgtcgcggt tgatttcaat gcggcccttc tcgccgatga agatgcaccc
     3901 gaggccgggg ccccagtcgg cgtcgaggct caaattgagc accgtgccgt tggcgtattt
     3961 catgcgcatc tttgcccggg gaccggtaaa ccgcgccatg ctgtaatacg ccgcacccgt
     4021 ttcctccgga gacggctgcc gctcctcaaa tgcgcctgag gggagagtct gaacagcttc
     4081 ctcgaggcag atctcggtcg gaccggtctc gtcagtgccg agtccgcgct ggatctggtc
     4141 gtagctgtgc gtaccccagc ccgtaacgcc gaacgacacc ccgccgccat cgtagtccca
     4201 ccagtgggcc cattgccggt gcaattccga atgatagggg cggaattccg cctggttgag
     4261 ccagagatcc caccagttgt cgctgccgcc ttcgggcatc ggctcggcgg gcttgggttc
     4321 ccagcgcagc ggcccgacga aattcggcgc gagcacttcg gtcacgcgcc caatcgcgcc
     4381 gttcttcacc agatcgctgg cccagttgtt gagggcgatc gtgcgctgct gcgtgccgac
     4441 ctgggtgacc cgctggtatt tgcgcgcgag caggaccatc tcgcgcccct cggcaatggt
     4501 cagacacatc ggcttctcga tgtagacgtc cattcccatg gccatcgcgt ggcaggccac
     4561 ccatgcgcgg gcatgcgtcg tggtttccac catgaccccg tcgaggtttt ccttctcgat
     4621 catttcgcgg aaatcggtgt agcccgtcca gccgcgatct ttgcccgcgc cgttgagaca
     4681 ggcgtccacc cgcggtttaa agcagtcgca gaccgccgta atccggattt cagggatatg
     4741 cagacaggtc cccgcgatgt cctggcagcg gccgcccaat ccgatcaggc ccacgttcac
     4801 cgtctcattc gggccgggcc cgtccatgcc aagcacccgg gtcgaaagaa tgagcggcgc
     4861 cgaggccgcg gcagtcttca aaaatgcgcg tctgctgatc atgggttatc tcctcgaagg
     4921 ccggtacgat gcgcggcacc ggcccgtcgt gcccgtggat gccgcggccg gatgcgcgcc
     4981 gtccgcggaa acatcagcct aacatccatg gctccccgta tcaaccgcag aacacgcccc
     5041 aatcacccgg cacgcgggca ccgcacaaaa aaacgccccc agagcgatgc ccgcgcccaa
     5101 cgcatcctcc ccgcaccgga ccccgtgccc ggcggctgag gtgtgtcgtc ccaccggatg
     5161 acttctccgg cagccatgcg ctacaataca cgttgatgcg cctgatcgcg gcgcatcacg
     5221 ccccgcgcgg agggttagcg taatggtaac gcaccggtct tgaaaaccgg cgccttcggg
     5281 cttgggggtt cgagtccctc accctccgcc acttcacaac ctcgctcacc gccccttttg
     5341 cccgaagacc catccagccc gcgagccgta cgcatgctga aatgcgcttc ttaacgacgg
     5401 tcattctccc ggaaggctct ttgtgtcagg gcaaacctgg gcgcagtcgt cgcacaaaaa
     5461 aagaaggact cacgcgcaat ggcgtgagcc cttaaccttt catggtgggc cgcgagggat
     5521 tcgaaccccc gaccttggga ttaagagtcc cctgctctac cacctgagct agcggccctc
     5581 gggttcgcag ggcggacccg tcagtgggat ttggaataaa aaaatggagc gggagacggg
     5641 atttgaaccc gcgacgtcaa ccttggcaag gt
//
LOCUS       DAOPBQ010000203         5671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12299_length_5671_cov_3.942308, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000203 DAOPBQ010000000
VERSION     DAOPBQ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12299_length_5671_cov_3.942308"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKW60_07800"
     CDS             <1..356
                     /locus_tag="PKW60_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPJ99373.1"
                     /translation="TFLSAVTLGKEAFPFLPVGLLLRHRFETEHKLVRCSAPILVVHS
                     PDDDVVPYHHGKGLFQLAPEPKTFLEISGNHNEGYAETGQRYIQGLCGFLDPLFPVEQ
                     GASDSDAGEGRAKMS"
     gene            complement(412..2232)
                     /locus_tag="PKW60_07805"
     CDS             complement(412..2232)
                     /locus_tag="PKW60_07805"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HPJ99374.1"
                     /translation="MADEGSVGIVTPQQFRFAEPPGQLELDCGRKLGPITIAYETYGT
                     LNADRSNAVLILHALTGDAHAAGFHSPSDPKPGWWDHMIGPGKGFDTNKYFVICSNCL
                     GGCRGTTGPYSINPENGRPFGLSFPMVTIGDMVRAQRQLIGHLGIERLLSVAGGSMGG
                     MQVLEWLTRYPELVRSGICIASGPHNGAQAIAFDAVGRHAVQADPAFKDGNYYGEDGP
                     VQGLSIARMLGHITYLSDEAMRAKFGRTLRGKSKYSYDFASEFSVETYLDHQGEKFVN
                     RFDANSYLYVTKACDYFDLVAAYGSLDEAFARVQSRVMVLSFTSDWLYPPYQSREMVD
                     SLMRNRKEVTYCNIHSDYGHDAFLLEVDAMRDLVAGFLEHTLEPERKTREAGPAVRGL
                     SNSTEPAPLSRHSIYESPRVDYDLIVDLVDPGSRVLDIGCGDGELLHRLVRLKQVDGL
                     GLEVSESNVVRCVNSGIPVVQADIDKGLSALPDASYDYVILSMTLQVIKKPELAIREM
                     LRVGKKCIISFPNFGFWKVRTLLAFRGKAPVTRSLPYSWHSTPNRNVLSVKDFRQFCE
                     QQQVRIEREIPLSSTGGGFMVRRWPNLFAEEAVYVVTAAN"
     gene            complement(2320..3618)
                     /locus_tag="PKW60_07810"
     CDS             complement(2320..3618)
                     /locus_tag="PKW60_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HPJ99375.1"
                     /translation="MTESRQHGLGTRALHAGQDPDPVTGSRAVPIYQTTSYVFNSAEH
                     AARLFGLEEFGNIYTRIMNPTVDVFEKRIAALEGGVGALGLASGSSAITLAALNITKS
                     GQNLVSSSTLYGGTFNLFAHTFKDLGIEARFVDAREPANFANAIDDKTRFLYCETIGN
                     PACDVPDIKAIADIAHKHGIPLIVDNTAASPILFRPIEHGADIVVHSATKVIGGHGTS
                     IGGVIVDGGTFDWNNGKFPELTEPCASYHGLKFYEAMKPLGNISYIIKARVTMLRDMG
                     PCLSPFNAFLLLQGLETIHLRVPRHSENALRVAQHLEAHPAVEWVSYPGLKSHPDHKR
                     AREYMPGGSGAILTFGVKAGYEGAKKFINGVKLASHLANILDAKTLVIHPASTTHQQL
                     SPEEQKEAGVTPNLIRVSVGIEDIEDIIADLDQALEQTAK"
     gene            complement(3663..4589)
                     /gene="cysK"
                     /locus_tag="PKW60_07815"
     CDS             complement(3663..4589)
                     /gene="cysK"
                     /locus_tag="PKW60_07815"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPJ99376.1"
                     /translation="MRVYDDNSFSIGNTPLVHLQRLGQGLPARIFGKVEGRNPGYSVK
                     CRIGASMVWDAEERGLLKPGGTIVEPTSGNTGIALAFVSAARGYKLVLTMPETMSLER
                     RQMLKAFGAKVVLTPGEQGMPGAIQEAEKIVQDNPGFFMPQQFNNPANPGIHFKTTGP
                     EIWNDLDGQADVLVSGVGTGGTITGVARYFKEARQKPLETVAVEPEASAVLSGGKPGP
                     HKIQGIGAGFVPGVLDLALIDKIETVSNEEAFETARRLAREEGLISGISCGAAAAVAL
                     RLGARPEYQGKNIVVILPDSGERYLSTPLFEG"
     gene            5035..>5671
                     /locus_tag="PKW60_07820"
     CDS             5035..>5671
                     /locus_tag="PKW60_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ99377.1"
                     /translation="MVKKSWHSGGLKRLFEQEWVRPLGTLLFVVVLATVFSPQRDGHL
                     VFWQSRNLFNVLRQISEIGILAVGMTLVIITGGIDLSVGSLLALASTLFAFTFVSHEW
                     SLGVSLAIALLAATLFGLVSGVLISRLRMQPFIATLAVMTGSRGFAKWLVENKTIDFP
                     MGEEYTARVADFLARQSVVIPTFACIALLGAILLNHTRFGRYAVAIGGSEEA"
BASE COUNT         1071 a         1668 c         1731 g         1201 t
ORIGIN      
        1 gtacgtttct ctccgcagtc actctgggta aagaggcgtt cccgttcctg cctgtgggcc
       61 tgttgctgcg ccatcggttc gaaacggagc acaagctcgt ccggtgcagc gctccaatac
      121 tcgtagttca cagtccggat gatgatgtgg tgccctatca tcacggaaag gggctgtttc
      181 agctggcccc tgagccgaaa acctttcttg agatctccgg caaccacaac gaaggatatg
      241 cggaaaccgg acaacgttac atccagggtc tatgcggttt cctcgacccg cttttcccgg
      301 tggagcaggg agcatccgat tccgatgctg gcgaagggcg cgctaagatg tcctgaacgt
      361 catccgcggg cgcggtgcac ctgctttttc acaaccgagg aggctaagcg gtcagttcgc
      421 ggcggtaacg acgtacacgg cctcttccgc aaacaggttg ggccagcggc gtaccatgaa
      481 cccgcccccg gtgctggaga gcggaatttc ccgttcgatg cggacctgct gctgttcgca
      541 aaattgccga aagtctttga cggataatac gttgcggttc ggtgtcgaat gccaggaata
      601 ggggaggctc cgggtgaccg gcgcttttcc ccggaacgcg agcagcgtcc ggactttcca
      661 aaatccgaaa ttcgggaagc tgatgatgca tttcttgccc acgcggagca tttcacggat
      721 cgccagctca ggtttcttaa taacctgcag ggtcatgctg aggatgacat agtcgtaact
      781 cgcgtccggg agcgcgctca ggcccttgtc aatgtccgcc tgaaccaccg gaataccgct
      841 gttaacgcag cggactacgt tactttcgga tacttcgagc cccaatccgt ctacttgctt
      901 gaggcggacc aggcgatgga gcagctcacc gtcgccgcat ccgatatcca gcacccggct
      961 gcctggatcg accagatcca caatgagatc gtagtccaca cgagggcttt cgtagatgct
     1021 gtggcgggag agcggcgccg gttccgtgga gttcgagaga ccgcgcactg ccggcccggc
     1081 ctctcgtgtt tttcgctcgg gctccagggt gtgttccagg aagcccgcga ccaggtctct
     1141 catggcatcg acttcgagca ggaacgcatc gtgcccgtaa tcggaatgga tgttgcaata
     1201 cgtcacttct ttgcggtttc gcatgagcga atcgaccatc tcacgcgatt gatacggggg
     1261 atagagccag tccgacgtga acgacagaac catgacacgc gactgaacgc gggcaaatgc
     1321 ctcatccagc gagccatacg ccgcgacgag atcaaaatag tcgcaggcct tggtgacgta
     1381 aaggtaactg ttcgcatcaa aacggttgac gaatttctct ccctggtggt cgagatacgt
     1441 ttcaacggag aactcgctgg caaagtcgta gctgtacttg gatttgcccc gcagagtccg
     1501 cccgaacttg gcgcgcatgg cctcgtcgga aaggtaggtg atgtgcccga gcatgcgggc
     1561 gatcgagagt ccctggacgg ggccgtcttc gccgtagtag ttgccgtctt tgaacgcggg
     1621 atccgcctgg actgcgtgcc ggccgacggc atcgaaggcg atggcctgcg cgccgttatg
     1681 cggtccggac gcgatgcaga tacccgaccg cacgagttca gggtagcggg tgagccattc
     1741 cagaacctgc atgccaccca tcgagccgcc cgccactgaa agtaggcgtt caatgcccag
     1801 gtgtccgatc aattgccgct gcgcgcgaac catgtcgccg atggtgacca tgggaaaaga
     1861 caggccaaac ggcctgccat tctcagggtt aatggagtag gggccggttg tgccgcggca
     1921 gccgccgagg caattggagc aaatcacgaa gtatttgttg gtatcaaaac cttttccggg
     1981 cccgatcata tggtcccacc agcccggttt cggatcggag ggagagtgga atccggcagc
     2041 gtgggcatcg cccgtcaggg catgcaggat gaggacggcg ttggagcggt cggcattcag
     2101 cgtgccgtag gtctcatacg caatcgtgat gggccccagc ttacggccgc aatccagttc
     2161 gagctggccg ggcggctcag caaaccggaa ttgctgcggc gtaactatcc caaccgagcc
     2221 ttcgtctgcc atccctgctc ccttgctgcc cgtcgcatct cgagataaaa aacgggcgcg
     2281 gtcccctgga aaaaggcacc gcgcctgccc agcataccat tatttggcgg tctgttccag
     2341 cgcctggtcc agatcggcga taatgtcctc gatgtcttcg atgccgaccg acacgcggat
     2401 caggttgggc gttacgccgg cttccttctg ttcctcaggc gaaagctgct ggtgcgtcgt
     2461 cgaggccgga tggatgacga gggtttttgc gtccagaatg ttcgccagat gcgaagccag
     2521 tttgaccccg ttgatgaatt tcttggcgcc ctcatacccg gccttcacgc caaaggtcag
     2581 gatcgcgccg ctcccgcctg gcatatattc ccgggcgcgt ttgtgatcgg ggtggctctt
     2641 caggccggga tagctcaccc attccaccgc cggatgcgct tccagatgct gggcgactcg
     2701 taacgcgttt tcggaatgcc tcgggacgcg caggtggatg gtttccaggc cctgcagcag
     2761 caggaaggca ttgaaggggg agaggcaggg gcccatgtcg cgcaacatcg taacccgtgc
     2821 cttgatgatg tacgagatgt tgcccagcgg tttcatggcc tcataaaact tcaggccgtg
     2881 gtaactcgca cagggctctg tcaactccgg gaatttgccg ttgttccaat cgaaggtgcc
     2941 gccatccaca atgaccccgc cgatcgacgt gccgtggccg ccgatcactt tcgtggcaga
     3001 gtgcaccacg atatcggctc cgtgctcgat cggccggaac agaatgggcg aggccgcggt
     3061 attgtcgaca atgagcggga tgccgtgctt gtgggcgata tccgcgatgg ctttgatatc
     3121 gggcacgtcg caagccggat tgccgatggt ctcgcaatac aggaagcggg tcttgtcgtc
     3181 gatggcgttg gcgaaattcg ccggctcgcg cgcgtcgaca aaacgagcct cgatgcccag
     3241 atctttgaag gtatgcgcga agaggttgaa cgtgccgcca tacagcgtcg acgaagacac
     3301 gaggttctgg ccggatttcg tgatgttgag cgcggccagg gtgatggctg agctgccgga
     3361 tgcgaggccc agggcgccca cgccgccttc aagcgctgct atccgctttt cgaacacatc
     3421 gaccgtggga ttcataatcc gcgtgtagat gttgccgaat tcttcgagcc cgaaaagccg
     3481 cgcggcatgt tcggcgctgt tgaagacata cgacgtcgtc tgataaatgg gcaccgcccg
     3541 cgagcccgtt acggggtccg ggtcctgtcc cgcgtgaagt gcgcgggtgc ctaatccgtg
     3601 ctgcctgctt tccgtcatct tcccttcctc tccctgtatc gtagtccttc caaaaccggt
     3661 acctaccctt caaacagcgg ggtagacaga tagcgctcgc ccgaatcggg aaggatgacc
     3721 acaatatttt ttccttgata ctctggacgc gcgccgagcc gcagcgcgac tgctgctgct
     3781 gcgccgcaac tgatgccgct gatgaggcct tcttcccggg cgagccggcg ggcggtttca
     3841 aatgcttcct cattggacac ggtctcaatc ttgtcgatca gggccaggtc cagtacgcca
     3901 gggacaaagc cggccccgat gccctgaatc ttatgcgggc cgggcttgcc gccagacagc
     3961 accgcggagg cttccggctc cacggcgact gtttcgaggg gtttttgccg ggcttcttta
     4021 aagtagcgcg caacgccggt aatcgtgccc cctgtgccga cgcccgacac cagcacatcg
     4081 gcctgtccat ccagatcgtt ccagatctcc gggccggtgg tcttgaagtg aatgcccgga
     4141 ttggccgggt tgttgaactg ctgcggcata aaaaaaccgg ggttgtcctg gacgattttc
     4201 tcggcttcct gaatcgcccc gggcatgcct tgctcccccg gcgtcaggac taccttggcg
     4261 ccgaacgctt tcaacatctg ccgccgttcc aggctcatgg tctcgggcat ggtcaggact
     4321 agcttgtatc cacgggcggc cgacacaaat gccagtgcga tgcctgtgtt gcccgaggtc
     4381 ggctccacaa ttgtcccgcc gggtttcagg aggccgcgtt cttccgcgtc ccagaccata
     4441 ctcgcaccga tgcgacactt gacggagtag ccgggattcc ggccttccac ctttccgaat
     4501 atccgggcag gcaggccctg gccgaggcgt tgcagatgga ctagcggggt gtttccgatg
     4561 ctgaagctgt tgtcgtcata aacacgcatc tcaatgtacc tctccggggc gctggaaccg
     4621 gtacgcttcc ccagtggagt cactgcctgt gccggctccc gttactgctg actgtttacc
     4681 atagcgaaaa aaacaaggct ggggcgcccg atgctatcac cccaaaccgt tatgatcaaa
     4741 cgctatggga aaacccgtgg cgcgcaggtg gaatcgcgcc gtacgaatcc gcgatgtcag
     4801 gaaaagaaac gcgccggtgg ctgtaagtat tccacaccag attggttact acacggaaca
     4861 ggaggcggca ggccggtgta tccgcaccga gggagggttg aaaccgcgca cttaaaacgg
     4921 gaaaataggc ccggcttgca ggggaagcca cggtcccgtt gctccggggc cttgggtggt
     4981 tttctgagca ctttcaagcg gcgcatgttg cccaggcacg gagttcgtac gcgcatggtg
     5041 aagaagagct ggcattcggg tggcctcaaa cggctcttcg aacaggaatg ggtgcggccg
     5101 ctcggtacgc tgctcttcgt tgttgtgctc gccacggtct tttctccgca gcgtgatggc
     5161 cacctggtgt tctggcaatc ccggaatctg ttcaatgtgc tgcggcagat ctcggagatt
     5221 ggcatcctgg cggttggcat gaccctcgtc attattaccg ggggcattga tttgtcggtg
     5281 ggatccctgc tggcgctggc gtccaccctg tttgcgttca cgttcgtgag tcatgaatgg
     5341 agcctgggcg tctctttggc gatagcgcta ctggcggcca cgctgttcgg cctcgtcagc
     5401 ggcgtgctga tctcacggct gcgaatgcag ccatttatcg ccacgctggc cgtcatgacc
     5461 ggcagccggg gctttgccaa atggctggtg gaaaacaaga ccatcgattt ccccatggga
     5521 gaggagtaca cggcccgcgt cgcggatttt ctggccagac agagtgtcgt gattcccacg
     5581 tttgcatgca tcgctctgct gggagccatt ctgctcaacc atacccgctt cggtcgctac
     5641 gcggtggcca tcggcggttc tgaggaggcc g
//
LOCUS       DAOPBQ010000204         5668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12310_length_5668_cov_3.781400, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000204 DAOPBQ010000000
VERSION     DAOPBQ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12310_length_5668_cov_3.781400"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PKW60_07825"
     CDS             complement(<1..962)
                     /locus_tag="PKW60_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99378.1"
                     /translation="MRSARAAVIFSLGFFAIAAQTFLFRQFLTAFEGNELAIGVFFCS
                     WLLWVTAGAWLARLFRRLTPIVTDWFRGAALLYLPAFVVQYGLLGQVRELAGIAPYEQ
                     FPLLIMLAVCIVANSPVSLLTGALFTWACRWTAGTESGSRSAALTPARVYMLEAVGAA
                     VGGVWATLWLAGGVPVETVFLLSALVVMAAGFLPNRLGLLRAAGVLVLAVAVALGAGE
                     SWSARSHQRQWRRLIPAGYEGRVTTAQAEYLYGEHDGQFAVMAWGSVCETPFTLSQGA
                     EDAAAILPQCPHARRVLVLGAGVYPLCTVLCSLPQIEQVAWFHP"
     gene            complement(959..2563)
                     /gene="amrB"
                     /locus_tag="PKW60_07830"
     CDS             complement(959..2563)
                     /gene="amrB"
                     /locus_tag="PKW60_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPJ99379.1"
                     /translation="MAKHPYMAGAAVVLAVAAVAIAVWMARREERSETPRPPLSQAQE
                     AEMPEQAPRPKHVFDSPLAGRWYPANPPALKEMLDLQFAAANQTPMDEVCALILPHAG
                     YQYSGPVAAFGLKAVSGRRPARIVLLGPSHRVPMRGYASVPGATHYRTPLGESELDAA
                     FIESLLQHDMFRRVAQVEELEHSVQILVPLIQHALGAVPLVPIVVGQLTLKESRAMAG
                     ILRDLVDADTLVVVSSDFTHYGPNYGYLPFRDDIPENLERLDMAAWKAIESKDPGAFE
                     TWVETNDATICGTCPIMVLLSMLPEEAGPHLLKYGTSGEITNDYENSVSYLSIAFTGS
                     WEKESPASDIAATALPLSQEDKAGLLRLARETLQTYVTEGRMPAPEELGIDISPAMKQ
                     IMGAFVSLHAHGTLRGCIGEIEPRRPLYEAVMAHAVNAGLKDYRFPSVTAAELPELEF
                     EISALTPPQPVDSPERIIIGRHGMTVEKAGHRAVFLPQIAPQQGWDRDTTLDHLCVKA
                     GLPADAWRQGAAFTVFEAVVFGEVTG"
     gene            complement(2780..3751)
                     /gene="amrS"
                     /locus_tag="PKW60_07835"
     CDS             complement(2780..3751)
                     /gene="amrS"
                     /locus_tag="PKW60_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPJ99380.1"
                     /translation="MTIQCELCPKNCLIAPGQSGECRIRVNIDGRLVAVTYGRPCAVH
                     VDPIEKKPLFHMLPGSRILSLATVGCNLHCKNCQNWEISQENPEKLAAKELDPGDVPA
                     LARQHACQSIAYTYTEPVVFYEYTLDGCRQAHDAGLKTALVTAGYINREPFERLAPHV
                     DAVNIDLKAMSDTFYRDLCEGSLAPVLNTLESAKSAGIWVEVTNLVIPTLNDSEADLQ
                     KLCRWVVSHLGRETPLHFSRFHPAFRMKNLPPTPEETLVRACDIARAEGLHYVYIGNV
                     ARAGAENTYCFQCGALVIERQRFTIIENRLRDGACPSCGTKVHGVWT"
     gene            complement(4058..5062)
                     /locus_tag="PKW60_07840"
     CDS             complement(4058..5062)
                     /locus_tag="PKW60_07840"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPJ99381.1"
                     /translation="MSQKVVAVAGVTGMVGRQMLQVLEERNFPVKSLKPLASERSRGK
                     SVVFRGEEIPVEVMTETSFKGVDIALFSAGGGTSKHFAPFAARDGCVVIDNSSAWRMD
                     PKVPLIVPEVNGADIAWHEGIIANPNCSTTQMVVVLKPLHDAATITRVVVSTYQAVSG
                     AGKAAVDEMYAQTRALLANEEPVCQIFPHPIAFNCIPQIPQSNAFGDNGYTSEEMKMV
                     NETKKIMGDDSIRVTATTVRVPVHTGHSESVNVETVNKLTAAQAREILSRAPGIVVED
                     DPAAQKYPLAIHAAGRGETFVGRIREDISHASALDMWIVSDNLLKGAAYNTVQIAELL
                     "
     gene            complement(5230..5550)
                     /gene="trxA"
                     /locus_tag="PKW60_07845"
     CDS             complement(5230..5550)
                     /gene="trxA"
                     /locus_tag="PKW60_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPJ99382.1"
                     /translation="MSSARELTRGDFDGVIGSGVTLVDFWAEWCGPCRMMGPAIEELA
                     AQYAGKATIAKVNVDNENDLAAKFNVSSIPTLLVFKDGKIAKQFIGVTSKTDLAKAID
                     DALA"
BASE COUNT         1066 a         1801 c         1777 g         1024 t
ORIGIN      
        1 tcggggtgga accacgccac ctgttcgatc tgcggcaggc tgcacagcac cgtgcacagc
       61 ggatacacgc ctgcccccag cacaagcacg cggcgggcat gcgggcattg cggcagaatg
      121 gccgcggcat cttccgcgcc ctgcgacagc gtaaacggcg tctcgcacac gctgccccag
      181 gccatcacgg caaattgtcc gtcatgctcg ccgtagaggt attcggcctg agcggtggta
      241 acccggccct cgtatccagc ggggatcagg cggcgccact ggcgctggtg gctgcgcgca
      301 gaccaggatt ctcccgcgcc cagcgcgacc gccacagcca gaacgagcac gcctgcggca
      361 cgcagcaagc ccaggcggtt gggcagaaac cccgcggcca tcacgacaag ggccgacaac
      421 aaaaacacag tctcgacggg aacgccgccg gcgagccata gcgtagccca gacgccgccc
      481 acggcagccc ccaccgcctc cagcatgtat acccgggccg gagtcagggc agcgctccgt
      541 gaaccgcttt cagtgccggc ggtccagcgg caggcccacg tgaacagcgc gccggtgagc
      601 aggctgaccg ggctgtttgc gacaatacag acggcgagca tgatcagaag cggaaattgc
      661 tcgtagggcg ctatccccgc gagttcgcgc acctgcccga gcaggccata ctgcaccaca
      721 aacgcgggga gataaagcag ggcggcgccc cgaaaccagt cggtcacaat cggggtgagc
      781 cgccggaata accgggccag ccaggcgccg gcggtcaccc agagcagcca ggaacagaaa
      841 aagaccccaa tggcaagctc attgccctca aatgcggtca aaaactggcg aaacagaaac
      901 gtctgtgccg cgatcgcgaa gaatccaaga gaaaatatga ccgcggcacg ggccgatctc
      961 atcccgtaac ctcgccaaac acgacagctt cgaaaaccgt gaaggcggcg ccctgccgcc
     1021 aggcatccgc gggcagtccg gccttcacgc agaggtggtc gagcgtggta tcgcgatccc
     1081 agccctgttg gggcgcaatc tgcggcagga acacggcgcg atggcccgct ttttcaacgg
     1141 tcatcccatg gcggccaatg atgatgcgct cggggctgtc gacgggctgg ggcggtgtca
     1201 gggccgagat ctcgaattcc agctcgggga gttccgcggc cgtgacactg ggaaaccggt
     1261 agtccttgag tccggcgttg acggcatggg ccatgacagc ttcgtacagg ggccggcgcg
     1321 gctcgatctc gccaatgcat ccccggagcg tgccgtgtgc gtgcagactg acgaacgcgc
     1381 ccatgatctg cttcatggcg gggctgatgt cgatgcccag ctcttccggg gccggcattc
     1441 ttccctcggt gacgtaggtt tgcaaggttt cacgagcgag ccgaagcagc ccagccttgt
     1501 cttcctgtga aagcggcagt gcggttgcgg cgatatccga tgcagggctt tccttttccc
     1561 aggagccggt gaacgcgatg ctgagatagc tgacggaatt ttcgtagtcg tttgtgatct
     1621 cgcctgaagt gccgtacttg agcagatggg gtccggcctc ctcagggagc atggagagca
     1681 gcaccatgat ggggcaggtc ccgcagatgg tagcgtcgtt ggtctcgacc caggtttcaa
     1741 aggccccggg atccttcgat tcgatggcct tccacgctgc catgtcgagc cgttcgaggt
     1801 tctcggggat atcatccctg aacggcaggt acccatagtt gggcccgtaa tgagtaaaat
     1861 cgctgctcac gactacgagg gtatcggcat ccaccagatc gcggagaata cccgccatgg
     1921 cgcgggattc cttcaacgtc agctgcccca ccacgatggg cacgagcggc accgcgccga
     1981 gcgcgtgctg aatcagcggc accaggatct gaacgctgtg ttcgagctcc tcgacctgcg
     2041 caacccgccg aaacatgtcg tgctggagca gggattcgat aaacgccgca tcaagctcgc
     2101 tttcgcccag cggcgtgcgg taatgcgtcg cgccgggcac gctggcatac ccgcgcatgg
     2161 gcacgcggtg cgatgggccc aggagcacaa tacgcgccgg ccgccggccg ctcacggcct
     2221 tcaatccaaa ggccgcgacg gggccgctgt actggtagcc cgcatggggc agaatgagtg
     2281 cgcacacctc gtccatcggc gtctggttcg cggccgcgaa ctggagatcg agcatttctt
     2341 taagcgcggg cggattggcc gggtaccagc gtcccgccag gggcgaatca aatacgtgtt
     2401 tcgggcgtgg ggcttgttcg ggcatttcgg cctcctgtgc ctgtgataga ggtgggcgcg
     2461 gcgtttccga gcgttcctcg cggcgcgcca tccagacggc gattgccacc gctgctacgg
     2521 ccaggacaac tgcggccccg gccatgtacg ggtgttttgc catacgtcac ctccccggcg
     2581 tctctatccg agcgcggcta cccggcccat cgggccgggc gcgcggtttc ctccggcgtc
     2641 aacgggacga aaacgccccg gcactcgcca cgctgcggct tttctgcgta ccggacacgg
     2701 atcagcgccc gtggcgcacg gcggcttccc ggccagagca gcgcggccgc cagcagtacc
     2761 ttcacaaatg tgcgccgtgc tacgtccata cgccgtgtac cttcgtgccg cacgaggggc
     2821 aggccccgtc gcgcagccgg ttttcgatga tggtgaaccg ctgccgctca atgaccagcg
     2881 cgccgcactg gaaacagtac gtgttttccg cccccgcacg ggccacattc ccaatataca
     2941 cgtaatgcaa cccttccgcg cgggcaatgt cacacgcgcg gaccaacgtc tcctcggggg
     3001 tcggcggcag gtttttcatg cggaatgcgg gatgaaaccg cgaaaaatgg agcggcgtct
     3061 cgcgacccag atgcgacacg acccaccggc agagtttttg gaggtcggcc tctgaatcgt
     3121 tcagcgtggg gatgaccagg ttggtcacct ctacccagat gccagccgat ttcgcgcttt
     3181 ccaaggtgtt cagcaccggc gccaggctgc cttcgcagag atcccggtaa aacgtgtcgg
     3241 acatcgcctt gagatcgatg ttgacggcgt cgacgtgcgg cgccaggcgt tcgaaaggtt
     3301 cccggttgat gtagcctgcc gtgaccagcg cggtcttgag cccggcatcg tgcgcctgcc
     3361 tgcatccatc cagggtgtat tcataaaaaa cgacaggctc ggtatacgtg taggcgatgg
     3421 actggcacgc gtgttgccgg gccagagcgg gcacgtcacc gggatccagc tcctttgcgg
     3481 ccagtttctc ggggttctcc tgcgaaatct cccagttctg gcagtttttg caatggaggt
     3541 tgcagcccac ggtggccagc gagaggatcc ggctccccgg cagcatatga aacaggggct
     3601 tcttctcgat cgggtccaca tgcacggcgc agggccgccc gtaggtgacc gcgaccagac
     3661 ggccgtcgat gttcacgcga atccggcatt cgccgctctg ccccggcgca atgagacaat
     3721 tcttgggaca taattcgcat tgaatcgtca tggcatcgca ctcaaacgtt ggcctctatt
     3781 gtgcgtcacc gggccagggg ggtcaaggcg gcggctcgcg ccgtttaaga tcggggcctg
     3841 tagtggcatc gtcgtgccat ttgcgatcgc atcgaaacag ggcaggcaca agggcctgcc
     3901 tttcttcaag acgaacgcac cggggtatgc caaggcagca tcaagcgctg acagacagac
     3961 acacaccgcc ggtccaggcg gcaccggatg tgccagttcc cggcacacgg gcagcacgct
     4021 cggcgtctac cgaatctcgc cgggttgggg gagcgctcta gagcagctcg gcgatctgga
     4081 cggtgttgta ggccgcgcct ttcaggagat tgtcggaaac gatccacata tccagcgcgc
     4141 tggcatggga gatgtcttcg cggatgcggc cgacgaaggt ctcgccccgg cccgccgcgt
     4201 ggatggccag gggatacttc tgcgccgctg ggtcgtcttc cacaacgata cccggcgccc
     4261 gggacagaat ctcgcgcgcc tgcgcagccg tcagcttgtt gacggtctcg acgttgacgg
     4321 attcgctgtg gccggtatgg acgggcacgc ggacggtggt cgcggtgacg cggatgctgt
     4381 catcgcccat gatcttcttg gtctcgttga ccattttcat ttcttcggag gtatagccgt
     4441 tgtctccgaa ggcattgctc tgcgggatct gggggatgca gttaaacgcg atcgggtgcg
     4501 ggaagatctg gcacaccggc tcttcgttgg cgagcagggc ccgggtctgg gcgtacattt
     4561 cgtcaacggc cgctttgccc gcgccggaca cggcctgata ggtcgaaacc acgacccgtg
     4621 tgatggtggc tgcatcgtgg agcggcttca acacgactac catctgcgtc gtcgagcagt
     4681 tcgggttggc aatgatgccc tcgtgccacg cgatatctgc gccgttcact tcgggaacga
     4741 tcagcgggac tttcgggtcc atgcgccacg cgctcgaatt gtcgatcacc acgcagccgt
     4801 cccgggctgc aaacggcgcg aaatgcttgc tcgtgcctcc tcccgcgctg aagagcgcga
     4861 tgtccacacc tttgaacgac gtctcggtca tcacctctac ggggatttcc tctccccgga
     4921 acacaacaga tttccccctg gaccgttcgg atgcgagcgg tttgagcgat ttgacgggga
     4981 aattgcgctc ttcgagaacc tgcagcatct gcctgccgac catgccggta acgcctgcta
     5041 cggcgacgac cttctgagac atgtgagtct ggctccttat acagttgcgg tgtggcgaag
     5101 caacaacaca gaaaaaccgc gcgtttcgca tcacgaaaca cacgtgcgcg tgcacatgcg
     5161 cggtacagca gcggcaaccg cccgaaacgc catccggcgc ccggcgcccg gccgccggct
     5221 gcgggcggat caggcgagag cgtcgtcgat ggcttttgcc aagtcggtct ttgacgtcac
     5281 tccaatgaat tgcttggcga ttttgccgtc tttgaacacg agcagcgtgg gaatgctgga
     5341 cacgttgaat ttggcagcca gatcgttttc gttgtcgaca ttcaccttgg cgatcgtcgc
     5401 tttgcccgcg tattgcgcag ccagctcctc gatggccggg cccatcatgc ggcaggggcc
     5461 acaccactca gcccagaaat ctaccagcgt cacgccactg ccaatcactc cgtcgaaatc
     5521 gcccctggta agctcgcgag cactgctcat gcgtaaaacc tcctgaaata acactgccga
     5581 tgaggcagag atcctctaca gcctccctgc cggcgatagt tcaaccctca aggaaaggcc
     5641 ggcgcaccga aaagtattcc cgcaaagg
//
LOCUS       DAOPBQ010000205         5666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12313_length_5666_cov_3.915167, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000205 DAOPBQ010000000
VERSION     DAOPBQ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12313_length_5666_cov_3.915167"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PKW60_07850"
     CDS             complement(<1..617)
                     /locus_tag="PKW60_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPJ99383.1"
                     /translation="MEILTMLKHGGWIMFPIGLCSLAGLAIIIERMVVLRRERILAPS
                     VIDLVEHFSEDTPPETALEICRSARGPFARIVEEIIGARHLPDAQVIETLQATGRTQV
                     STMERGLTLLEIIGNVSPLLGLLGTVLGMVTVFNAISVEGVGHPRVLSEGISKALITT
                     VAGLIVAIPAVAFHSWLSARVDDMATEMHERATAFVSKLHYVRLRA"
     gene            complement(699..1685)
                     /locus_tag="PKW60_07855"
     CDS             complement(699..1685)
                     /locus_tag="PKW60_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HPJ99384.1"
                     /translation="MHTRESDNYGQRMLRASAAAVVIHIALIALFSAVGAVTVVDAIG
                     PHIAREEPIVLNLEPDPQGFRHFVDTAVPAASPVDPDADLISDQASKASGLRESEGER
                     PGPRLEQQAEIDDLAGSATPAPVVAPAPLAPQADATEPAAQPDTPATTPESPAQPQPE
                     ALTALARAGELRPPLPAEAPEPAPQQMAQTEPQPASEPAAMPQPHLGVSKARPGGAAE
                     GKGFIGFEALEHELGPYLKEVRDRVERNWRAALEMRFSGASRTQAVLDCAISPQGKLV
                     RVKIVEPGDSPTFAVLCKQAIEKAAPFSPFPFDVPAIYRSQNLEIRWTFSYL"
     gene            2029..3426
                     /locus_tag="PKW60_07860"
     CDS             2029..3426
                     /locus_tag="PKW60_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPJ99385.1"
                     /translation="MSNAFKFINPKFVPPLDPGFRPAVLANRAFVNEVKESGAGVPLV
                     LGLERADGSLSRFETVVFPEGHPRAEANLQYVERLVKFLLWQRGGWKVYVGGPKSVGA
                     YVAQCYSPDGSRSFDYHFMGEDVYEKTFTVVSCDAADVPPENETTQPLGRHLEGCRIG
                     FDLGASDRKVSAVVDGEAVYSEEVVWEPRQNSDPSYHYEEVMKAIKTAASKMDRVDAI
                     GGSSAGVYINDRPMIASLFRGVPKEKYDQVRSMFHRIRDEMGVPLNVVNDGEVTALAG
                     SMSLEDNGVLGIALGSSEAGGYVTMDGNITGWLNELAFCPVDYSEEAPADEWSGDRGV
                     GALYFSQQCVFRLAPRAGIELLTGVTDAERLKAVQDKLEAGHEGAVNIWRSMGYYMGY
                     AIAHYADFYELKHVLILGRCTSGRGGDLILEGANEVLAAEFPQIAATVNIQLPDEKSR
                     RVGQSIAAASLPVLP"
     gene            3545..4393
                     /locus_tag="PKW60_07865"
     CDS             3545..4393
                     /locus_tag="PKW60_07865"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPJ99386.1"
                     /translation="MKFKLETAELFVPDGLPARDALARTTHLAVGAHQDDLEIMSFAG
                     IMECFQQSDKWYTGVVVTNGSGSPRDDVYKNYTDDEMRVVRFKEQKKAAVVGEYAAQI
                     LLDYPSSAIKSAADKNPVDDLRLILEATKPDVVYTHNLADKHDTHVGVTLKLIEAIRG
                     LPKDQRPNQVLGCEVWRDLDWMADADKVALDASAHENLQAALLGVFDSQICGGKRYDL
                     ATMGRRKAHATYFASHGVDLTTGMNFSMDLTPLITDDSKDPGAYVQEFIQRFADEVKA
                     RMAKLA"
     gene            4474..5466
                     /locus_tag="PKW60_07870"
     CDS             4474..5466
                     /locus_tag="PKW60_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPJ99387.1"
                     /translation="MKRIRQQRRPDARIASVLCAVAIGLCGLCGACGGQGQIPVQNGR
                     GEEVHRMPPPEAVSGHERIITFAPHITETVFALGKGALVVAVSDFCDFPPEIEGLERV
                     GGWHNPNLEKITRLNPDLIIVQGEHERVTQLAAKRGFELLRVNMDSLATIREGIRLIG
                     DRLGCPEEAKALVRGFDADLASIRECVAAHPRMTVLILTGRQLHDMTDFHTVGGTSFV
                     SELVATAGGDNLYQGETQPYLEASKETAVMRAPEAIIEFHAGETFSDDIKAQYLTDWQ
                     QMPSLPAVRNHRIYLITESHALRPGPRVSEIAARLAALLYPDLEGVLRGHGQMP"
     gene            5450..>5666
                     /locus_tag="PKW60_07875"
     CDS             5450..>5666
                     /locus_tag="PKW60_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017600160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPJ99388.1"
                     /translation="MGRCLESQRVSFAYEPEAPVLAEADVSVTGGKLLGVIGPNGSGK
                     STLLRLLCGFLQPVRGAVLLDGRPLSAL"
BASE COUNT         1081 a         1673 c         1871 g         1041 t
ORIGIN      
        1 gcccgcaacc ggacgtaatg cagcttcgag acgaaggcgg tggcgcgttc atgcatttcg
       61 gtggccatgt cgtccacgcg ggcgctgagc cagctgtgaa acgcgacggc agggatggcg
      121 acgatgagcc ccgccaccgt ggtaatcagt gctttggaga tgccttcgga gagaacgcgc
      181 gggtggccga cgccttccac tgagatcgca ttgaacacgg tgaccatacc gagcacggtg
      241 cccagcaggc cgagcagcgg gctgacgttt ccaataattt cgagcaaggt caggccgcgc
      301 tccatcgtgc tgacctgggt gcgcccggtg gcctgcagcg tctcgatcac ctgggcatcg
      361 gggaggtggc gcgcgccgat gatctcttcc acaattcggg cgaacgggcc gcgcgcactc
      421 cggcagattt cgagggccgt ctcgggcggc gtgtcttccg aaaaatgctc gacaaggtca
      481 atgacgctgg gggcgagaat gcgttcgcgg cggagcacca ccatgcgttc aatgatgatc
      541 gccagccccg ccagcgaaca caggccgatg gggaacatga tccatccgcc atgtttaagc
      601 atcgtcagga tttccacagg cagacttcct ttctcgaacg gggatggtgc ctcatggcgg
      661 ccggattttg caacaggcag cgaggcgcag cgcccggttc acaaataact gaacgtccag
      721 cggatttcga ggttttgcga tcggtagatc gcgggaacat cgaacggaaa cggggaaaac
      781 ggcgcggcct tttcaatggc ctgcttgcat agaaccgcga aggtcgggga atcgcccggc
      841 tccacgattt tgacgcgaac aagcttgccc tgagggctga tcgcgcagtc gagaacggcc
      901 tgggtgcgcg atgctcccga gaaacgcatt tcgagcgcgg cgcgccagtt gcgctcgaca
      961 cgatcgcgga cttctttgag ataaggcccg agttcgtgct ccagcgcctc gaaaccgatg
     1021 aagcctttgc cttcggccgc accgccgggc cgcgccttgg acacacccag atggggctga
     1081 ggcattgcgg ctggctcact ggcgggctga ggctcggttt gcgccatctg ctgaggggcg
     1141 ggttcgggcg cttcggcggg gagcgggggt cggagttcac ccgcacgggc aagtgccgtg
     1201 agagcctccg gttgcggctg ggccggtgat tccggcgttg tggcgggcgt atccggttga
     1261 gctgccggct ccgtggcatc ggcttgaggt gcgaggggag ccggcgcaac gaccggcgcg
     1321 ggagtcgcag atcccgccag gtcatcgatc tcggcctgct gttccaggcg tgggcccgga
     1381 cgctcgcctt cggattcgcg cagaccactg gctttggatg cctgatcgga aatcagatcc
     1441 gcatccgggt cgaccggaga cgcagccggc acggccgtat ccacgaaatg gcggaaaccc
     1501 tgcggatcgg gctccaggtt gagcacaatg ggctcttcgc gggcgatgtg cgggccgatg
     1561 gcatcaacta ccgtcaccgc gcccacggcg ctgaacaagg caataagggc aatatggatc
     1621 actacggcgg cggcggacgc gcgaagcatg cgctggccgt aattgtcaga ttctcgcgtg
     1681 tgcatgattc tccgattgta tcagaccgcc tgtcgcgcgg ggaatggaat gaagcgcttc
     1741 aggcgcaagt tggctcgatg agcgccagta gaaagcccgg cgtgatgagt gggttttttc
     1801 ggcgtttctt ttgtactgcg gaggtatggt atagttgccg caccctttat gaaacgctgg
     1861 cagaatcttt gtccggccgt tccgcatggc cggccgggcg cccgcgcgga agactccggt
     1921 ccatggtgac tctacacgcg tctgagcgtc agcgatcatg cggcgggtac atgcccacta
     1981 cggcgcctgc tgcgtgagag aatacagcca aaacaaagga aggtagtcat gtcgaacgcg
     2041 ttcaaattca tcaatccgaa atttgtgcct ccgctggatc cgggatttcg cccggcggtc
     2101 ctggccaacc gcgcctttgt gaacgaggtg aaggaatcag gcgccggcgt gcccctggtg
     2161 ctggggctgg aacgcgcaga cggctcgctg tcgcgcttcg agaccgtggt gtttccggaa
     2221 ggccatccgc gggcggaagc aaatctccaa tacgtcgagc ggctggtgaa atttctcttg
     2281 tggcagcgcg ggggatggaa ggtctatgtc ggtggaccga agagcgtcgg cgcgtatgtc
     2341 gcccagtgct attcgcctga cggcagccgc tccttcgatt accacttcat gggtgaggac
     2401 gtttatgaga agaccttcac cgtcgtgagc tgcgatgcag cggatgtccc gcctgagaat
     2461 gagaccacgc aaccgctggg ccggcacctc gaaggctgcc ggatcgggtt cgacctgggc
     2521 gcctccgatc gcaaggtgtc agccgtggtc gacggcgagg cggtatatag cgaagaagtg
     2581 gtctgggagc cgcgtcaaaa ctccgatccc tcctaccact acgaagaggt catgaaagcc
     2641 atcaagaccg ccgcgtcgaa gatggaccgc gttgacgcca tcggcggcag ctcggcgggc
     2701 gtgtatatca acgaccgccc catgatcgcg tcgctgtttc gcggggtgcc taaagagaaa
     2761 tacgaccagg tccggagcat gttccaccgc atccgcgacg agatgggcgt gcccttgaac
     2821 gtcgtcaacg acggcgaggt cacggcgctt gccggctcga tgtcgcttga ggacaacggc
     2881 gtgcttggca tcgccctggg ctcgagcgag gcgggcggtt acgtcaccat ggacggaaac
     2941 atcacgggct ggctcaacga actggcgttt tgcccggtgg actacagtga agaggcgcct
     3001 gcagatgaat ggtccggcga ccggggcgtg ggcgcgctgt atttctcgca acagtgcgta
     3061 tttcgtctgg cgccgcgggc cggcatcgag ctgctgacag gcgtgaccga tgccgagcgc
     3121 ctgaaagcgg tccaggacaa actcgaagcg ggccacgaag gcgcggttaa catctggcgc
     3181 agcatgggct actacatggg ctatgccatc gcccattacg ccgatttcta cgaattgaaa
     3241 catgtgctga ttctcggccg ctgcacgtcg ggccggggcg gcgacctgat tctcgaaggc
     3301 gcgaacgagg tgctcgctgc cgaattcccg cagattgccg caacggtcaa cattcagttg
     3361 cccgacgaga agagccggcg cgttggtcaa tccatcgccg cggccagtct ccccgtgctt
     3421 ccttgaggcg gccggggccg gaaacgcaaa atattccggc ggccgtgagt gtttcggggt
     3481 gcgccggggt gcgattgggt atcattcaac gaggtcgcgg aatgcgcgat ttcgggagga
     3541 gagtatgaag ttcaaactgg agaccgcgga actttttgtg ccagacgggc tgccggcccg
     3601 ggacgcgctg gcacggacca cccacctggc cgtgggtgcg caccaggatg acctcgaaat
     3661 catgtccttc gcgggcatca tggagtgctt tcaacaatcc gacaaatggt ataccggcgt
     3721 ggtcgtgacc aacggcagtg gttcgccccg cgacgacgtc tacaaaaatt atacggacga
     3781 cgaaatgcgc gtcgtgcggt tcaaggagca gaaaaaggcc gccgtggtcg gcgaatatgc
     3841 cgcgcagatc ctactggact accccagttc ggccatcaag agcgctgcgg acaaaaatcc
     3901 cgtggacgat ctcaggctga tccttgaggc cacgaagccc gacgtggtgt atacacacaa
     3961 tctggccgac aagcacgaca cgcacgtcgg cgtgacgctg aagctcatcg aggcgatccg
     4021 cgggcttccg aaagatcagc gccccaacca ggttctcggc tgcgaagtgt ggcgcgacct
     4081 cgactggatg gcggatgcgg acaaggtggc cctggacgca tcggctcacg aaaacctcca
     4141 ggcagcgctg ctcggcgtgt tcgattctca gatctgcggc ggcaaacgct acgacctggc
     4201 caccatgggc cggcgcaaag ctcacgccac ctactttgcc tcgcacggcg ttgacctgac
     4261 caccggcatg aatttcagca tggatctgac cccgttgatt actgacgaca gcaaagatcc
     4321 cggcgcatac gtgcaggaat tcatccagcg ttttgcggat gaggtcaagg cgcgtatggc
     4381 caaactcgcg taagcatgcc atccggggcg gccgcaccac ttcgcggccg ccctgcttta
     4441 tgtccggcct cgcgacccgg aggcgcgaac caggtgaaac ggatacgaca acagcgccgt
     4501 cccgatgcac gtatcgcgag tgtgctgtgt gcggttgcca ttggcctgtg cgggctctgt
     4561 ggcgcctgcg gcggccaggg ccagattccc gtgcagaacg gccgggggga agaagtccac
     4621 cgaatgccgc cgcccgaagc cgtctccgga cacgagcgca tcatcacatt cgcgccgcac
     4681 atcacggaga cggtgtttgc gctcggaaaa ggcgcgctcg tggtggcggt gtctgatttc
     4741 tgcgatttcc cccccgaaat cgagggtctc gagcgtgtcg gcgggtggca caatccgaat
     4801 ctcgagaaga ttacccggct gaatcccgac ctgatcattg tgcagggcga gcacgaacgc
     4861 gtgactcaac tggccgccaa acgcggcttt gaactgctcc gggtcaatat ggacagcctg
     4921 gcaaccatcc gggaaggcat ccggctcatc ggcgaccgcc tgggatgccc tgaagaagcg
     4981 aaggccctgg tgcgggggtt cgatgcggat ttggcgtcga tccgggaatg cgtggcggcg
     5041 catccgcgca tgaccgtgct cattctcacc gggcgtcaac tgcacgacat gaccgacttt
     5101 cacacggtcg gaggtacgtc gttcgtgtcg gaactggtcg ccacggccgg cggcgataat
     5161 ctctaccagg gcgaaacaca gccatatctc gaagcctcca aggaaacggc cgtcatgcgc
     5221 gcgccggagg ccattatcga atttcatgcg ggagagacct tttcggatga catcaaggcg
     5281 cagtatctca cggactggca gcagatgcct tcgcttccgg ccgtgcgaaa tcaccggatc
     5341 tatctcataa cggagtcgca tgcgttgcgc ccggggcctc gcgtgtcgga gatcgcagcc
     5401 aggctcgccg cattgttgta tcctgacctg gaaggtgtgc tgaggggcca tgggcagatg
     5461 ccttgaatca cagcgcgtat cgtttgcgta tgagcccgag gcgccggttc tggcagaggc
     5521 cgatgtgagt gtgaccggcg gtaagctcct gggcgtcatc ggaccgaacg gttccgggaa
     5581 aagtactctt ctgcgcctcc tctgcgggtt tctccaaccg gtccggggag ccgtgctgct
     5641 ggatggccgg ccgctgagcg ccctgc
//
LOCUS       DAOPBQ010000206         5660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12329_length_5660_cov_3.858341, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000206 DAOPBQ010000000
VERSION     DAOPBQ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12329_length_5660_cov_3.858341"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKW60_07880"
     CDS             <1..493
                     /locus_tag="PKW60_07880"
                     /inference="COORDINATES: protein motif:HMM:NF037386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPJ99389.1"
                     /translation="RTTMIVGLPGETPAQHRRMLEGIRELRFDWLGAFSYSREPGTPA
                     AEAPRQVGARVRLKRWEAVMAAQAEIAEEKSRARIGGVERVLVEGYDPDRGLWFGRSG
                     REAPEVDGTVLLQAGTALEPGHFVSAKIIGAETYDALAQVLEAPATNAQVCTGNAEGK
                     DGA"
     gene            complement(637..1584)
                     /locus_tag="PKW60_07885"
     CDS             complement(637..1584)
                     /locus_tag="PKW60_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPJ99390.1"
                     /translation="MDTNRLTRREALRSGAALALVPGIATFTTAGQSGAPAQWPKIGV
                     STLGFGDCSNRELAEEFASKEVRTIQLFFNQTDNQYWAYNGRSDVSDITPDRAKAIAN
                     EYRSRGIDIHSIGVYTNLIHPEASERAANLDYFEAMMRIGAEMDVHTFITEAGHYQTQ
                     DSVPYHFQTDTWNRTVETVRELAKRAEACNATVLMEPTFLSFFASAKRTRVFLEEVDS
                     PRIRALLDPANILEINDLEEMFAQLSLWIDCIHAKDRKLHVDFGVPAGQGDIDYRKFA
                     ALAAKHTPNAPLILEYVGTNDYLQAREVLLNAIRSLGEA"
     gene            1832..4804
                     /locus_tag="PKW60_07890"
     CDS             1832..4804
                     /locus_tag="PKW60_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99391.1"
                     /translation="MFGLQEWRGSGVFFLAAIGLLSAICCVAAYAEEAGVPDDVYRGE
                     LVAYPGPWGFMPRPHIILVNDEELEAIASDPDKILNLATGYAPQEESLRMICERTQKQ
                     GLRTLIVAFDQFFAQYRPGQDKPRRLMPDMDEYVGHIAKIGAFAAEYGLGLELSLLSP
                     LEIGPAYARETGESGQWMHYRKGLRDVETGRFSVQLWRQTRWTNNKGPIDIADAGVRV
                     FAFRERGIRGTPYRVVKPDEIVEITDVASVDVWDGAKTPLAQRIRVHGEGRTDIGALD
                     RVLVVQVYRTPEMDYFSPNALPFLKELVDQYADAGIQLNGLYADEMHIQQDWSYFGHH
                     DHGEFALRYVSDGLVNAYASTYGEEYRDFAKYLVYFVRGQEDFAPDLSAKAGVMHVFG
                     GTPEEIRRTALFRARYYHLLQDGVVDLFTAAKRHAEARMGRRLESRAHATWAESPTID
                     RWDTGRAPQAPQQYEYTSNFIWSCTVHQAASACYDYFKWGDFLTGNGNDHAEGGWLDR
                     NYFAMALACSTGILNEVPYAYAAHWGMPGPISAWRTSLVNTYGTGGSPLWGMVEDMQH
                     RDVDVLMLYPLDLVAVDERFGSWMTQYGYANYVSAAKLLERGRVSNGAIEMAGRRFST
                     LVALFEPFPAKALLEMAGALVATGGRVVWSGPPPVLTYEEDNALEAWQDLFGVAYAPD
                     FDEGLLAPGRRVEFEGSLDGVEAQEILTDFLVDRIYPVEPQAGTSAAARVAGRVVGTV
                     RAGEGGGMAAFLGYRPRDDQSQSLGYETRNWFGVLTALGAYPPTGAFSGVNDNTEYLS
                     RTTEYLACRFPNGAVAIARHFRTFEEGWPGGFARKEEEDNAYMEQRPLPDDTIRLERF
                     QVNGHEVEYEGKQAMAFRVNERGELIAFAGSQAREVTIDGRQFQFADAPFWQVAWAPV
                     SEERQVDGGALLQIMAHGQGLLRIPKGDLPDELTIVAQGPTPGSRGHEVPSQWDAGAL
                     VFEVTGKESGRWLWVVPK"
     gene            5447..>5660
                     /locus_tag="PKW60_07895"
     CDS             5447..>5660
                     /locus_tag="PKW60_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99392.1"
                     /translation="MWLLAVLLLGVTPWASAVPYFGDEVFTYYQPDGSTFGVRLYGDE
                     FFAYQRTADDAREVVLDEATGFWCYAK"
BASE COUNT         1054 a         1545 c         1911 g         1150 t
ORIGIN      
        1 ccggaccacg atgattgtgg gattgccggg cgaaaccccg gcccagcacc gccggatgct
       61 cgagggcatc cgcgaactcc gctttgactg gctgggggca ttctcctact cgcgcgaacc
      121 cggcacgccc gccgcagagg ccccgcgcca ggtgggagcg cgtgtgcgtc tgaagcgctg
      181 ggaggccgtg atggccgcgc aagccgagat tgccgaggaa aagagccgcg cgcgcatcgg
      241 cggggttgaa cgcgtgctgg tcgaaggata cgacccggac cgcggactct ggtttggccg
      301 gagcggccgt gaagcgcccg aagtcgacgg aacagtgctg cttcaggccg gtacggcgct
      361 tgagccgggg cactttgtct ccgccaaaat catcggcgcc gaaacctatg acgcgctggc
      421 ccaggtgctc gaggcgcccg ccacgaatgc gcaggtatgt accggtaatg ccgaggggaa
      481 ggacggcgca tagcctcgga ccgccattgt tcccgggcta agcgcagcga cgcgccctgc
      541 cctgaacgcc gggatttctc cgccttaccc gcgtgtctta cgcaaggcgg ccagtctatg
      601 aaaccagctt cgattgtgcg gcccgaaccc gcgcgatcac gcttcgccca ggctgcgtat
      661 cgcgttcaga aggacctccc gcgcctggag ataatcgttg gttcccacgt attcgaggat
      721 caacggggcg tttggggtgt gtttggcggc aagggccgcg aatttccggt agtcgatgtc
      781 gccctggcct gccggtaccc caaaatcgac atggagcttg cgatccttgg cgtggatgca
      841 gtcaatccag aggctcagct gggcaaacat ctcttcgaga tcattgatct cgagaatatt
      901 ggcgggatcc agcagagcgc gtatgcgcgg cgaatccact tcttcgagga aaacgcgggt
      961 gcgcttggcg ctggcgaaga agctcaggaa ggttggctcc atgagcacgg tggcattgca
     1021 ggcctctgcc cgttttgcga gttcccggac cgtctcgact gtccggttcc aggtgtcggt
     1081 ctggaagtgg tagggaacgc tgtcctgagt ttggtaatgg ccagcctcgg taataaaggt
     1141 gtggacgtcc atctcggcgc cgatacgcat catggcctcg aaatagtcga ggttcgcggc
     1201 acgctcggat gcttcggggt ggatcaggtt ggtgtacacg ccgatgctgt ggatatcgat
     1261 accccgggac cggtattcgt tcgcgatcgc cttggcgcgg tcgggagtga tgtcggacac
     1321 gtcgcttcgg ccgttgtagg cccaatactg gttgtcggtc tggttgaaaa agagctggat
     1381 ggtgcgcacc tctttcgagg cgaattcttc agcgagttcg cggttgctgc agtcgccaaa
     1441 gccgagggta gatacgccaa tcttgggcca ttgggcgggg gcgccggact gtccggcggt
     1501 tgtgaaggtg gctatcccgg gcaccagggc cagcgcggca cccgagcgca gggcttcgcg
     1561 gcgtgtgagt cggtttgtgt ccatggcgga taccctccct gaatgaacag cattgtcgtg
     1621 gatgcccggt tccatcttgc cacaatgcca gcacggaata cagatcaacc gcggagaccc
     1681 gtgttgaaat cgagatgaga gggcccaggg ggctgtctgt tcggctgcgg tgggtgttgt
     1741 ctgcgtagtg gggcgtttgc gcggcggctg ccgggcagat agtatgggcg gagaatctgt
     1801 tagcgcggaa ttcgatgcaa aggggaatcc tatgttcggg ttgcaggaat ggcggggttc
     1861 aggtgtgttt ttcctggcgg cgattggtct tctttcggcg atctgttgcg tcgctgcgta
     1921 tgcggaggaa gcgggcgtcc cggatgatgt ctaccgggga gaactggtgg cctatcccgg
     1981 gccctgggga ttcatgccac gcccgcatat catcctggtg aatgacgaag agctggaagc
     2041 gatcgcgtcc gatccggata aaatccttaa tctggccacg ggttatgcgc cccaggaaga
     2101 gagtctccgg atgatctgcg aacggacgca aaaacaggga ttgcgaacgc tgattgtggc
     2161 gtttgaccag tttttcgccc aataccggcc cggacaggac aaaccccgcc gattgatgcc
     2221 cgatatggac gaatatgttg ggcatattgc aaaaatcggc gcgttcgcgg cggagtatgg
     2281 cctggggttg gaattgagcc tgttgagccc gctggagatc gggcccgcct atgcgcggga
     2341 gaccggcgag tcgggccagt ggatgcatta ccggaaaggc ttgcgggacg tcgagactgg
     2401 ccggttcagc gtccagctct ggcggcagac ccgctggacc aacaacaagg gccccatcga
     2461 tatcgcggat gcaggggtgc gcgtgtttgc gttccgcgag cggggcatcc gcggcacgcc
     2521 gtaccgcgtg gttaagccgg atgagatcgt cgagattacg gacgttgcct cggtggatgt
     2581 gtgggacgga gcgaagacgc cgctggcgca gcgcatccgg gtgcatggcg aaggccgtac
     2641 ggatatcggt gcgctcgacc gggtgctggt ggtgcaggtc taccgcacgc cggagatgga
     2701 ctatttcagc ccgaacgccc tgcctttcct gaaagagctg gtggaccagt acgcagacgc
     2761 gggaattcag ttgaatggct tgtacgcgga tgaaatgcat attcagcagg actggtccta
     2821 ttttggccac cacgatcacg gggagtttgc gctgcggtat gtcagtgacg gcctggtgaa
     2881 tgcctacgcc agcacgtatg gcgaagagta ccgcgatttt gcgaaatacc tggtctattt
     2941 cgtgcggggg caggaagatt tcgcgcccga cctgagtgcc aaggcaggcg tcatgcatgt
     3001 ctttggaggt acgcccgaag agatccgccg gacagcgctg tttcgcgcac ggtattacca
     3061 tctgctgcag gacggagtag tggatttgtt cacagccgcg aaacgccatg ccgaggcacg
     3121 gatgggccgg cgcctcgaat cgcgggcgca cgctacgtgg gcggaaagcc cgaccattga
     3181 ccggtgggat accgggcggg cgccacaggc gcctcagcag tacgaataca cctcaaactt
     3241 catctggtcg tgcacggtgc atcaggcggc gtcggcgtgc tacgactatt tcaagtgggg
     3301 cgacttcctg accggcaacg gcaacgacca cgccgaaggc ggttggctgg accgcaatta
     3361 ctttgccatg gcgctcgcgt gttcgacggg gattctgaac gaggtgccgt acgcgtatgc
     3421 ggcccactgg ggcatgcccg gccccatcag cgcctggcgg accagcctgg tcaataccta
     3481 cgggaccggg ggctccccgc tgtggggcat ggtggaggat atgcagcacc gggacgtcga
     3541 cgtgctcatg ctgtatcccc tcgatctggt cgccgtggat gaacggtttg gaagctggat
     3601 gacgcaatac gggtacgcca actacgtcag cgcggcgaaa ctcctcgagc gcgggcgcgt
     3661 gtccaatggc gcgatcgaga tggccggccg gcgtttctcg acgctcgtgg cgttgttcga
     3721 accgtttccc gccaaagcac tgctcgaaat ggccggggcc ttggtcgcta ccggggggcg
     3781 cgtggtgtgg tccggaccgc ctccggtgct gacgtatgaa gaggacaacg cgctggaggc
     3841 ctggcaggac ctgttcggcg tcgcgtatgc gcccgacttc gatgaaggct tgcttgcacc
     3901 gggacgccgg gttgagtttg aaggcagtct ggatggcgtc gaagcgcagg aaattctgac
     3961 ggactttctg gtagaccgca tctatccggt tgagccgcaa gcagggacca gcgcggcggc
     4021 gcgggtggca ggccgggttg tgggaacggt acgtgccggc gagggcggag gaatggcagc
     4081 ctttctgggc taccgtcctc gagatgatca gtcgcaaagc ctgggttatg agacacgcaa
     4141 ctggttcggc gtcttgacgg ctctgggcgc ctatccaccc accggagcgt tttcgggcgt
     4201 aaacgacaat acggaatacc tgtcgcgcac cacagagtat cttgcctgcc gttttccaaa
     4261 cggcgccgtg gcgatagcgc ggcatttccg tacttttgaa gaggggtggc cgggcggttt
     4321 tgctcgaaag gaagaggaag acaacgccta catggaacag cgtcccctgc cggacgacac
     4381 gatccgcctg gagaggttcc aggtgaatgg ccatgaagtg gagtacgaag gcaaacaggc
     4441 catggcgttc cgtgtgaatg agcgcggtga gttgatcgcg tttgccggat cgcaggcccg
     4501 ggaagtcacc atcgacggcc gccagttcca gtttgcggat gcccccttct ggcaagttgc
     4561 atgggcgccc gtatctgaag agcgccaggt tgacggcggg gcgttgcttc agatcatggc
     4621 tcatggccag gggctcctgc ggataccaaa aggcgatctg ccggatgagt tgaccatcgt
     4681 ggcgcaagga cccacgccgg gcagccgcgg acacgaggtc ccctcccagt gggacgccgg
     4741 ggcgctcgta tttgaagtga cgggtaaaga atccggacgc tggctctggg ttgtccccaa
     4801 ataaataatc gataccctga aacaggatgc catcggcgca gatccccggg ccattcgcct
     4861 tacgggattt ccgagtctgc tttcgtcgcc tatctaactg aagggcctgt cctgccttcc
     4921 atgtgccggg aaacgacctc ggtttctgcg tttgccgctg cgctgctccc ccgcagaccg
     4981 cccaaaaaac ctggctcgct aatcgctacg tgaaacctcc ttcagccagg ccggaaattc
     5041 acgataaccc cagatttttg tacgctatga catagggaag gctctttgat agtgcgagct
     5101 tccaatgggc tgtgcgagag tggtcctgcg cgagacaatg caccgacttg tcgggtatga
     5161 gggacaaccg gggagtctgt gcaagagttt cgattttttt tgcgggattt tccgggcaat
     5221 agctgtttca ttttgctgtg caaggcacca atctgttgac agaattcagg gcctctgcta
     5281 gtgtgcttaa aatgcgaatg tggctaggta cgaagtgttt ggaagttgca catttttcgg
     5341 ggcaggcaat aatcaggccg taatccaaca gaagggaaca tcggtaatgt gtaagctcgc
     5401 atcgtgcaat actccttacg taagtgcttc gggacggaat cgtgctgtgt ggttgctggc
     5461 ggtcttgttg ttgggcgtga cgccctgggc ctcggcggtg ccctatttcg gcgacgaggt
     5521 gttcacctac tatcagccgg acggctcgac cttcggggta cggctgtatg gcgacgagtt
     5581 cttcgcgtac cagcgtacgg cggatgacgc gcgtgaagtg gttttggatg aggccacggg
     5641 attctggtgc tacgcgaaac
//
LOCUS       DAOPBQ010000207         5659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12331_length_5659_cov_4.231977, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000207 DAOPBQ010000000
VERSION     DAOPBQ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12331_length_5659_cov_4.231977"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..4092
                     /locus_tag="PKW60_07900"
     CDS             376..4092
                     /locus_tag="PKW60_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPJ99393.1"
                     /translation="MNPADDAMATAAPRPEDVRWHPAMFQPLHTSPLRVLFADDEEPL
                     VVSSLELFKREGWEGEGVTSGAAVLEHLERDAFDVVVLDYTLAGSEGEQLHQRLADRH
                     PSLPVIIITGCPSIESAIASMQRKAFDYLTKPLDMEYLLKRAREAAEQYRLRHALQHG
                     KQRYQSLIDDVLEVSQVALLLVDAEHRIAWINRAMERFFGLQRQRVLGRDVRAFIVDE
                     LAPHLAHPAEFQRMMVAAYDNNAYPEHFECRVLESGDRKGRWLEHWSQPIVSGLYAGG
                     RIEQYVDITTRKRAEEDLLANREHQDTAPDGSPDWEIWLGVAGELLYTSPSCQRFTGY
                     EVQEFMDDPSLLTRIIHPDDRPALAQHYREDIVSPECARFDFRIITKDGRKRWLHHVC
                     QPAYGTDGQPLGRHAWFQDITVRHALEEQLRKREAILEGIGFTAGQLLRDESWERSIS
                     KVLERLGAAAHVSRITIFKNHQLESGEFLMRPHASWVDPGVSGTLETPLPEGLPYRAA
                     GLARWETVLSHGECLSGITRQFPPQERQFLMEQDIASILLVPLFAGRQWWGFIGFEES
                     VRERVWTRTEIAALRAAADSISAAIQRELTESALRSKEAFLRGIQEVMLDAVEVIDSE
                     GTIVWRNTLAGEIYGNSVGSKCYENLNRTVRCPDCAHTAIQYDGVPRDYECEVVDTRG
                     RKRIMWVRATPFHGSKGGISAIVETSRDITERKRMEGALLESQRRLELALEGSGLGLW
                     DWDVKRGRVYRDQGWNTLLGYSAETVEEHSFEPYWPAHPEDIAQAAEAWDSHLQGHTA
                     FCEARVRMKRETGAWRWMLVRGQVVERDEDGTPVRATGTIRDITEAVLAEREQKRLSE
                     RMRQAHKLESLGILAGGVAHDFNNLLMGIMGNAHLALTSLPQSSPVRQHLTEIEHIGR
                     RAAQLANQMLAFSGQSRFAMRPLSLSEMIRDMAPLLTACVPRRIHLRYELRDDLPVVL
                     ADDRQLRQMIIALLDNAREAIGNRAGEIRIRTTSIRADSRYLANTYVDDALAPGEYLV
                     LELSDDGPGIPAEIVPKIFDPFFTTKLTGRGLGLAAVLGIIRGHNGAIRVDSQAGTGS
                     KFVVLLPSTQVKPAEERAAAPAPAVEWRGQGLALLVDDEDAVLRVGEGMLKRLGFEVL
                     KAYDGVEALEIYDRHSEAIVCVILDLTMPRMGGEEAFLQLRSRKPDLPIILSSGYSEM
                     DVVGRFTGKRLTAFIHKPYTGDELREILRKTLTE"
     gene            complement(4120..5289)
                     /gene="galK"
                     /locus_tag="PKW60_07905"
     CDS             complement(4120..5289)
                     /gene="galK"
                     /locus_tag="PKW60_07905"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPJ99394.1"
                     /translation="MSISRQEAVSRFKSHFRTLPTLGVRAPGRVNIIGEHTDYNHGFV
                     LPMAIERDTVIVARPLDAPIIRACAANLNRVAEAPLDRFERNPAEPWLDYIVGVAREL
                     ALLGRPLYGAEMMILGDVPVACGLSSSASLEMAALCLFEQLGGFTLEGPEAPRLGQRV
                     ENDFLGLSTGIMDQFISRMGRAGHVLFLDCRSFEYELIPASFDSACFAVANTGVARGL
                     TASKYNERVAQCREAVEGMRQTLGKTGSHLRDFTIEDLEAARASLSGIAYRRARHVIT
                     ENTRTRAACGAMRAGDLDGLGRLMNASDESLRVDYEVTCPELDAMTEIARSIPGCFGS
                     RMTGAGFGGCTVSLVANAEIEPFKARLAREYHARTGLPCDILVTGAAGGAGALPL"
BASE COUNT         1109 a         1704 c         1841 g         1005 t
ORIGIN      
        1 tcttgtacgt ttctctcgca gcccgtacaa tggtctctgg ttgcggatat ccaaaagacg
       61 agaaagatgt tacggaaacc ccgggagcag ggcgtcaatc cgtggaccat acagcccctg
      121 cccggcccgg gcaagcaatg gaaaagaagg aatgcagcag agtaacgcct caacgatgcc
      181 gccttcctca ggagaccggc gcgggcaggc tttgcggcct gcgagccacc cgggcttggg
      241 ccggtctgtt tcgcggggcc tcgcgggcac gatgccgcca gaattcaccg taaacgcttc
      301 tccgacgggc agcacgctgt tcggcctgtt cctgccggtc acaccgccgc taccgcgaat
      361 agagaggacg gcgccatgaa ccctgccgat gacgcaatgg ccacagcggc accgcggccg
      421 gaagacgtcc ggtggcatcc agcgatgttt caaccattgc acaccagccc cctgcgggtc
      481 ttgttcgcgg acgatgagga gccgctggtg gtttcgtcgc tggagctgtt caaacgggag
      541 ggctgggaag gcgagggcgt cacgagcggc gccgctgtcc tcgaacatct tgagcgcgat
      601 gcctttgacg ttgtggtcct ggattacaca ttagcgggca gcgaaggaga gcaattgcac
      661 cagcggctcg cagaccggca tccgtccctg ccggtcatca tcatcacagg atgcccctcg
      721 atagaatccg cgattgccag catgcagcgg aaggcgtttg attaccttac caaaccgctg
      781 gacatggaat acctgctcaa acgggcgcgg gaagcggcgg aacaataccg gctgcggcat
      841 gcgcttcagc atggcaaaca gcggtatcaa tcgttgattg acgacgtgtt ggaggtttct
      901 caggttgcgc tcctgctcgt tgacgcagaa caccgcatag cctggataaa ccgcgcgatg
      961 gagcgttttt tcggtctcca gcggcaacgg gttctgggcc gggacgttcg cgcgtttatc
     1021 gtggacgagc tcgccccgca ccttgcgcac cctgcggaat tccaacgcat gatggtcgcc
     1081 gcctacgaca acaacgcgta tccggagcat tttgagtgcc gcgtgctcga gtcgggcgac
     1141 cgtaaaggcc ggtggctgga acactggagc cagccgattg tgtccgggct gtatgccggg
     1201 ggaagaatcg aacagtatgt cgacatcacg acccgcaaac gcgcggaaga agatctcctg
     1261 gccaacaggg aacaccagga cacggcaccc gacggcagcc ccgactggga gatctggctc
     1321 ggagtcgcgg gagaactgct ctacacctcg ccgtcatgcc aacgtttcac ggggtatgag
     1381 gtgcaggaat tcatggacga tccttcactc ctcactcgga tcattcatcc ggacgaccgt
     1441 cctgcgctgg cgcagcatta ccgggaagac atcgtctctc cggagtgtgc ccgctttgat
     1501 ttccgcatca tcacgaagga cggccgcaaa cgctggctcc atcatgtctg ccagcccgca
     1561 tacggaacgg atggccagcc tctgggccgg catgcctggt tccaggacat caccgtacgg
     1621 cacgcactgg aagagcagtt gaggaagcgg gaggccatcc ttgaaggcat cggatttacg
     1681 gccggacagc tgcttcgcga cgagagctgg gaacgttcca tcagtaaggt gctggagcgg
     1741 ttgggagcgg ccgcccatgt gagccgcatt accatcttca agaaccacca gctcgagagc
     1801 ggcgagttcc tcatgcggcc gcacgcttca tgggtcgatc ccggcgtgag cggaacgctc
     1861 gagacgccgt tgccggaagg actcccctac cgcgctgcgg gcctggcgcg ctgggaaacc
     1921 gttttgtccc acggcgaatg cctttcgggg attacgcgcc agtttccccc gcaggaacgg
     1981 caatttctta tggagcagga catcgcgtcg attctgctgg tgccgctgtt tgccgggcgg
     2041 cagtggtggg ggtttatcgg gtttgaggaa agcgtccggg aacgcgtctg gacccgcacg
     2101 gaaatagctg ccctgcgcgc cgcggcggac agcatcagcg cggcgatcca gcgggagctg
     2161 accgagagcg ccctgcgcag caaggaggcg tttctccggg gcattcagga agtcatgctg
     2221 gatgccgtcg aggtgatcga cagcgaaggg acgatcgtct ggcggaatac gcttgcgggc
     2281 gaaatctatg gaaacagcgt gggcagcaag tgctatgaga acctgaaccg cacggttcgc
     2341 tgcccggatt gcgcccacac ggccatccaa tacgacggcg tcccccggga ttacgaatgc
     2401 gaagttgttg acacgcgagg ccggaaacgc atcatgtggg tccgcgccac gcccttccac
     2461 ggctcgaagg ggggcatatc ggccattgtg gaaacgagcc gtgacatcac ggaacgcaaa
     2521 cgcatggagg gcgccctcct ggagagtcag cgccgcctcg agctggccct cgaggggagc
     2581 ggcctgggtt tgtgggactg ggacgtgaaa cgaggacggg tctaccggga tcaagggtgg
     2641 aacacgctgc tgggatattc cgccgaaacc gtggaggagc acagtttcga gccttattgg
     2701 cccgcgcatc cggaagacat cgcgcaggcc gcagaggcat gggacagcca ccttcagggg
     2761 catacggcgt tttgtgaagc ccgggtccgc atgaaacgtg aaacaggggc atggcgatgg
     2821 atgctcgtcc ggggccaggt ggtcgaacgg gacgaggatg gcacgccggt ccgggccaca
     2881 ggcacgattc gggatattac cgaggccgtg ctcgccgaac gggagcagaa gcgactgtcg
     2941 gaacgcatgc gccaggccca caagctggag agcctgggaa ttctggctgg cggcgtggct
     3001 catgatttta acaatctgct gatggggatc atgggcaacg cacatctggc cctcaccagc
     3061 ctgccccaga gttccccggt gcggcagcat ctgaccgaga tcgagcatat cgggcggcgg
     3121 gccgcccagc tggcgaacca gatgctcgcg ttttccggcc agagccggtt tgcgatgcgg
     3181 ccgttgagtt tgtcggagat gattcgcgat atggcgcctc tgttgacggc gtgcgttccg
     3241 cggcgcatcc accttcggta cgagctgcgc gacgatctgc cggtggtact ggccgacgac
     3301 cgccagctcc gccagatgat cattgcgctt ctggacaatg cccgggaagc tatcgggaac
     3361 cgtgccggcg aaatccgtat ccgcacaact tccatcaggg ccgattcacg ctatctcgcc
     3421 aacacctacg tggatgacgc gctggccccc ggcgaatacc tggttctgga gctatcagac
     3481 gacgggccgg ggatccccgc tgaaattgtt cccaagatct tcgatccatt cttcaccacc
     3541 aaattgacgg ggcgcgggct cggacttgcg gcggtgcttg gtatcatccg cggccataac
     3601 ggcgcgattc gcgtagacag ccaggcgggc accggctcga agtttgtggt tttgctgccc
     3661 agcacccagg tgaaaccggc ggaagagcga gccgcggcgc ccgcaccggc cgtggagtgg
     3721 cgcggccaag gcctggcgtt gctcgtcgac gatgaagatg ccgtcttgcg cgtgggcgaa
     3781 ggcatgctga aacgcctggg tttcgaggtc ttgaaagcct atgacggcgt agaggcgctg
     3841 gaaatctacg atcggcattc tgaggctatc gtgtgcgtca ttcttgacct caccatgccg
     3901 cgcatgggag gtgaagaggc ctttttgcag ctgcgctccc gaaaacccga tctccccatc
     3961 atcctgtcca gcggttacag cgaaatggac gtggtgggcc ggtttacggg caagaggctc
     4021 acggcattca tccacaaacc ctacacgggc gatgaactcc gggaaatcct gcgcaagacg
     4081 ctgactgaat gagccgcagg atctgcgcca cctggcattc tacagcggaa gcgcaccggc
     4141 gcccccggcc gcgccagtca ccagtatgtc gcagggcaat ccggtgcgcg catggtattc
     4201 acgggccagc ctcgccttga acggctcgat ctccgcgttg gccacgaggc tgaccgtaca
     4261 tccgccaaaa ccggccccgg tcatgcggga gccgaaacac ccgggaatgc tgcgggcgat
     4321 ctccgtcatg gcgtcgagtt ccggacaggt gacttcataa tccacccgca ggctttcatc
     4381 ggaggcgttc atgaggcggc cgaggccgtc caggtcgccc gcgcgcatgg ctccgcaggc
     4441 ggcgcgggtt cgcgtgtttt ccgtaatgac gtgccgggcg cgccggtacg cgatgccgga
     4501 gagagaggcg cgcgcggcct ccagatcctc tattgtgaag tcgcggagat ggctgcctgt
     4561 ctttcccagg gtctggcgca tcccttcaac ggcttcgcgg cactgcgcaa cgcgctcatt
     4621 gtacttcgaa gcggtcagtc cgcgggccac gcccgtgttg gcgacggcga agcacgcgct
     4681 gtcaaatgag gcgggaataa gctcatactc gaaactgcgg caatccagaa ataggacgtg
     4741 gccggccctg cccatccgtg agatgaactg gtccatgatg ccggtactca gccccaggaa
     4801 atcattttcc acgcgctgtc ccaggcgcgg ggcttccggc ccctccaggg tgaacccgcc
     4861 cagctgttca aagaggcaca gggcggccat ttcgaggctg gcggaactgc tcagaccgca
     4921 cgcgacgggg acatcgccga ggatcatcat ttcggcgccg taaagcggcc tgcccagcag
     4981 cgccagctcg cgcgccacgc cgacgatata gtcgagccag ggctcggcgg gattgcgctc
     5041 aaaacgatcc agcggagcct ccgccacgcg gttgagattg gcggcacagg cccggatgat
     5101 cggggcatcg aggggccggg cgacaatgac cgtatcacgt tcgatggcca tcggcagcac
     5161 aaaaccgtgg ttgtaatccg tatgctctcc gatgatgttg actcggccgg gcgcgcgcac
     5221 gccgagcgtt gggagcgtcc tgaaatggct tttaaaccgg gagactgctt cctggcggct
     5281 gatactcacg gtcatgtccc ctttgtagtg aatcggccgg gttgcgcggc aaactccagc
     5341 gaatattgcg gtccagcgtc catttcgagt gcggcaggca ccgggtaagc ctatcatacc
     5401 atcggtgtta cgcagcgccc aagcgaccgg tctgaaaatg caattggcgg cggcctgaaa
     5461 tcttgatgcg gcctgtgggt tcgcgtagga tgaggctcac ggcgcaggag ggatggtatg
     5521 aagcatacga agctggcact gttgggcggg aaaccagcgg tgccggaggg cacgatccgt
     5581 acgcggcccc cgatcgattc gagcgatgaa gaaagagtgc tggcatcact ccggggcaaa
     5641 aaccacgcct acggcccca
//
LOCUS       DAOPBQ010000208         5657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12336_length_5657_cov_3.453767, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000208 DAOPBQ010000000
VERSION     DAOPBQ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12336_length_5657_cov_3.453767"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="PKW60_07910"
     CDS             <1..1099
                     /locus_tag="PKW60_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPJ99395.1"
                     /translation="VLPAAGELHACAQHCTLLYQELLNLGLQEEEGESSIDAEILQLG
                     GYRDRMQRLANLLAESTGARLEPNTVRWIEIDARNDRHLRVARCPLHVGQELSEWVYG
                     NLQSVVMTSATLTVDHSFDYLFGRIGLDNVRSRAVVAEALDSPFDYRIQAMLGIPTDI
                     STPDSDGFLPEVVEYVRAILEITRGHAFVLFTSFYSLNYVYGRLEDSLTAQGITPLKQ
                     GSAARTHLLERFRRDASSVLFGTDSFWEGVDVAGDALQCVILPKLPFRVPTEPILEAR
                     AEAIEAAGGSSFMDYTVPQAVVKFRQGFGRLIRRKTDRGAVIILDRRILTKHYGKVFL
                     RSLPEPQRVTGTRQVLLPSLETFFGESRGDT"
     gene            1096..2463
                     /locus_tag="PKW60_07915"
     CDS             1096..2463
                     /locus_tag="PKW60_07915"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="catalyzes the formation of D-fructose 6-phosphate
                     from D-glucose 6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPJ99396.1"
                     /translation="MNLDIRIDVSRARDAAVGRTHGITRAELDDLQPRIADAHEALQR
                     ERAEEKYGFWDLYKQAGVFDKVKAAAARFSQTGYDNLVVLGIGGSSLGTIALSAALLS
                     PYHNLLSRGSRKGIPRLFVMDNIDPGVFREMMRVCPPKKTLYNVISKSGETAETMCQL
                     MIIVEAIEKKLGRDAMKNHVVVTTNPRQSDAPPSLLHPVADEYGLTEFAVPLNVGGRF
                     SVFSPVGMFPAAMLGMDLDAMRDGCASMDLRCGQPSLDANPAYLRAAVQYLADKNKGK
                     SISVMMAYANGLYSIADWYRQLWAESLGKRHNLDGEEIFAGQTPIKALGATDQHSQIQ
                     LYREGPNDKLINTLETQKFARTLRIPPVLTGIAPLEYLRGSSMNALMAAELRGTLDAL
                     KLSQRPVSRIILPRINAHTVAQVLYMLEVETAMAGRLYNVNTFDQPGVEEGKKIARKL
                     MGGEG"
     gene            2460..4064
                     /locus_tag="PKW60_07920"
     CDS             2460..4064
                     /locus_tag="PKW60_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99397.1"
                     /translation="MREHPPKFSAYGDSGDLIGARRTRWAFRYCVIACVMLVAVLWIS
                     EYFIGYERAENLYLSALTKHKESARPLLRQAVLRDKAGSEAPNPRYVQALAERELEDE
                     VLPTYQQAFELDPGNATLALRYGCRLFMAGQFEQARDRFRDAALYAPDNALPRYLEAT
                     TIPWTLHEEPNILRESLKLIAQANSSGKQIVFPRPLWHPKLPPQGRQYAQLRREAIEE
                     CVAPIAQYRDYIMGIAARQIKEKHTQYWDSWLQTLERAGDKLARSALLPDSEGDSGAG
                     SASQAMVGLDLQLRAVRLRQQILEMESGQPDEALKQRESELEGGLAALRDFENARQPV
                     IERDSTAREFACLTAPFFAVFALSVFCALGALAARLLGATRYDWTIPHGRAGLLIMAS
                     YALMLGVFLACAPAVQQATARDAVPGLRLVWMILLAATGVSALIYPSLVLPSPKKSAA
                     EDGFGAMTSSERVSAARMRFHRAYAAMARRFLGVTLGLFLCVFSLWALVFRIATSLYP
                     WQVELLATGMGTQEARMVRQALSLLH"
     gene            4267..4851
                     /locus_tag="PKW60_07925"
     CDS             4267..4851
                     /locus_tag="PKW60_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPJ99398.1"
                     /translation="MSDHEQKAFTTFEAAKICHVTHHSIKNWIKQGLIKASRTPGGHY
                     RILEEDLDSFREKYDMFPRDKGPMKKRVMIVEDEPDTLALMENILMDEGFELIKVSNA
                     TEVGLRAAQLSPDLILLDYLMPEINGFEVSKALRNNELTRSIPIMAVTCLTKEQDIER
                     IFECGADEYLAKPFRFDQLLEKVRDLIGRGRIVE"
     gene            4873..>5657
                     /locus_tag="PKW60_07930"
     CDS             4873..>5657
                     /locus_tag="PKW60_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99399.1"
                     /translation="MSASGPRRGIERKILNTILQVAILPLIIAVIVGYITAREGQGNA
                     VEQSLLTAARKTADGLRIAAAPHLQKLDALSKDPKILAAVQPGSSGKPAALQDSAQHE
                     QLREYLNSQITMVVSTPGVPTILTVYDAEGHLIASTHEELHTLTSEDNGPGKWKDSRI
                     VKPRFLDIDEIGNAYAAESAAPIYQNPEDREGLLGFVAQILDITPLLLFAMGIDPTDT
                     ADDSLHAVYQVVYTSEGYHRTTYIARKSEQGTLNLDVCRTDSAL"
BASE COUNT         1205 a         1731 c         1588 g         1133 t
ORIGIN      
        1 tgtgcttccc gcggccgggg aactccatgc ctgcgcccag cactgcaccc ttctctacca
       61 ggaactgctg aatctgggac ttcaggaaga agagggggaa tcctcaattg acgccgaaat
      121 cctgcaactg ggcggatacc gtgaccgcat gcagcgcctt gccaatctcc tcgcggaaag
      181 caccggcgcc agactcgaac ccaataccgt gcgctggatc gaaatagacg ctcgaaacga
      241 ccggcatctg cgtgtggcgc ggtgcccact gcatgtgggc caggaactct cggagtgggt
      301 atacggcaat ttgcaaagtg tcgtaatgac ttcggcgacc ctcaccgtcg accacagctt
      361 cgattacctg ttcggcagaa tcggcctgga caatgtgcgt tcccgcgcgg ttgtcgcgga
      421 agctctcgac tctcctttcg attaccgcat ccaggcgatg ctgggcatcc ccactgacat
      481 ctccacgccc gattcagacg gttttctgcc cgaagtggtg gagtatgtgc gggccatcct
      541 tgagataacg cgcggccatg cctttgttct gttcacgtcg ttttattcgc tcaattacgt
      601 ctatggccgg ctggaagaca gcctgacggc acagggcatc acccccttga agcaggggag
      661 cgccgcccgc acccatcttc tcgaacgttt tcgccgggat gcctccagcg tcttgttcgg
      721 cacggacagt ttctgggagg gggtggacgt tgcgggcgac gctttacagt gtgttatact
      781 gccaaaattg ccgtttcgag tgcccacgga gcccatcctc gaggcccgcg ctgaagccat
      841 cgaagcggcg ggcggcagtt cgttcatgga ctatacggtg ccccaggccg tcgtgaaatt
      901 ccgccagggc tttggacgcc tcatccggcg gaaaaccgac cggggcgcag tgatcatcct
      961 ggaccgacgc attctcacaa aacattatgg aaaagtcttt ctgcggtccc tgccggagcc
     1021 gcagcgagtc accgggacgc ggcaggttct cttgccgtcg ctcgaaacat tcttcgggga
     1081 aagcaggggt gacacatgaa tctcgatatt cgcatcgatg tctcacgagc ccgggatgcc
     1141 gcggtggggc gcacccatgg gattacccgg gcggagcttg acgatctcca gccgcgcatc
     1201 gcagacgcgc atgaggccct gcaacgggaa cgcgccgaag aaaaatacgg tttctgggac
     1261 ctctataagc aggccggcgt cttcgataaa gtcaaagccg ccgccgcccg attctcacag
     1321 acggggtacg acaatctggt cgtgctgggc attggcggct cttccctggg cacgattgcg
     1381 ctcagcgctg ccctgctctc gccgtaccat aacctgctga gccgggggag ccgcaaaggc
     1441 attccgcgcc tgtttgtcat ggacaacatt gatcccgggg tattccgcga gatgatgcgt
     1501 gtttgccccc cgaaaaagac gctttacaac gtcatttcaa agtcgggcga aaccgccgaa
     1561 accatgtgtc agctcatgat tatcgtcgag gccatcgaaa agaaactcgg ccgcgacgcc
     1621 atgaaaaacc acgtggtggt cacgacgaat ccgcgccagt ccgatgcacc acccagcctc
     1681 ctgcaccccg tggcggacga atacggcctc acggaatttg cggtgcccct caatgtcggt
     1741 gggcgcttct ctgtcttttc tcccgtaggc atgttccccg cggccatgct cggcatggat
     1801 ctcgacgcca tgagagacgg atgcgcctcg atggacctgc gctgcgggca acccagcctc
     1861 gacgccaatc cggcctatct tcgcgcggca gtccagtacc tggccgacaa aaacaagggc
     1921 aagagcatct ctgtcatgat ggcttacgcg aacgggctct acagcatcgc cgactggtac
     1981 cggcagttgt gggctgagag cctgggtaaa cggcacaacc tcgacggaga ggagattttt
     2041 gcgggccaga caccaatcaa ggcgctcgga gccacggatc agcattcgca gatccaattg
     2101 taccgggaag gccccaacga caagcttatc aatacccttg aaacgcaaaa gtttgcccga
     2161 actcttcgaa ttccccccgt cttgaccggg attgcaccct tggaatacct gcgtgggtcc
     2221 agcatgaacg cgctcatggc tgctgagctc cgggggacgc ttgacgcgct gaaactgagc
     2281 cagcgcccgg tttcgcgcat catccttccc cggattaatg cacacacggt cgcccaggtg
     2341 ctttacatgc ttgaagtgga gaccgccatg gctggccggc tctacaacgt caacaccttt
     2401 gatcaacccg gggtcgagga aggcaagaaa atcgcccgga aactcatggg aggagagggg
     2461 tgagagaaca ccctccgaaa ttctccgcct atggcgacag cggcgacctc attggcgcgc
     2521 gccggacgcg ctgggcgttc cggtactgcg ttattgcctg cgtgatgctg gtagctgtcc
     2581 tgtggatttc tgagtatttt atcggttacg aacgcgctga aaatctctat ttgagcgcgc
     2641 tcaccaaaca caaggaatca gcccggccgc ttctccgcca ggctgtcctg cgcgataaag
     2701 cgggcagcga agccccgaat ccccgttatg tgcaagcgtt ggcagagcgc gaacttgagg
     2761 acgaggtgct tccgacgtac cagcaggcct ttgaactgga ccctggaaac gccaccctgg
     2821 cccttcgcta cggctgccgg ctcttcatgg ccgggcaatt tgaacaggcg cgtgaccgtt
     2881 ttcgggacgc cgccctgtat gcccccgaca acgcgctgcc gcgatacctc gaagcgacca
     2941 ccattccctg gactctccat gaagagccca acattctccg tgaatccctc aaactcatcg
     3001 cccaggcaaa cagcagcggg aaacagattg tcttccctcg acccctctgg catcccaaac
     3061 tgccgccaca aggccggcaa tacgcccaat tgcgccgcga agccattgag gagtgtgttg
     3121 cccctatcgc tcaataccgc gattacatca tgggcatcgc cgcccggcag ataaaggaaa
     3181 agcacaccca atactgggat tcctggctgc agaccctgga aagggcaggg gacaagctgg
     3241 ctcgcagtgc gcttctgcct gattctgaag gtgattccgg cgcgggaagt gcctctcaag
     3301 ccatggtagg gctcgactta caactgcgtg cggtgcgcct gcgccagcag atcctcgaaa
     3361 tggaatccgg acagcccgac gaggccttga aacagcggga atccgaattg gagggcggcc
     3421 tcgcggcgct tcgtgatttt gagaacgcgc gtcagcccgt catcgagcga gacagcaccg
     3481 cccgcgagtt tgcctgcctg accgcgccgt ttttcgccgt ttttgccctg agcgtctttt
     3541 gtgctcttgg ggcgctggcc gcgcgcctgc tgggcgcaac ccggtacgat tggaccatcc
     3601 ctcacggccg ggcgggcctt ctcatcatgg catcgtatgc gttgatgctc ggcgtgtttc
     3661 tcgcgtgcgc gccggcggtc cagcaagcca ctgcaagaga cgccgtcccc gggctgcgcc
     3721 tggtctggat gatcctcttg gctgcaacag gtgtttctgc gctgatctat cccagcctgg
     3781 tgctcccgtc tccgaaaaag tccgccgcag aggacggttt tggcgccatg acgtccagcg
     3841 agcgcgtgtc tgccgccaga atgcgctttc accgcgcata cgccgccatg gcacggcgtt
     3901 tcctcggggt tactctgggc cttttcctgt gcgttttctc gctctgggcc ctggtgtttc
     3961 gaatcgcaac atccttgtat ccgtggcaag tggagctgtt ggccactggg atggggaccc
     4021 aggaagcccg gatggtccgc caggctctct cgttgctaca ctgattgagt gacttgagcc
     4081 gtatatcaag aaatctagtg acgaaaacgg aaaaaaggga tgaatccacg agcattaagc
     4141 actccttgaa tttgacaaag ggctttccca gagctatact ttccatatag tgagtatact
     4201 gctaatttcg tacgccttcg tttcgtgccg atgaagattt tctacacacg tggaggttag
     4261 tatctcgtga gcgaccacga acaaaaggct tttaccacct tcgaagcagc aaagatctgt
     4321 cacgtaaccc accacagcat aaagaactgg atcaaacagg ggttgatcaa agcctctcgc
     4381 acgcccggcg ggcattaccg gatcctcgaa gaggatctcg acagtttccg cgaaaaatat
     4441 gacatgtttc cgcgggacaa gggacccatg aagaaacggg tcatgattgt tgaggacgaa
     4501 cccgacacgc tcgccctgat ggagaacatc ctgatggatg aggggttcga actgatcaag
     4561 gtcagcaatg caacagaagt cggcctgcga gccgcccagc tttcacccga cctgattctg
     4621 ctcgattacc tgatgccgga gatcaacgga tttgaggtat ccaaggcatt gcgcaataat
     4681 gagctgacgc gcagcatccc cattatggcg gtgacctgcc ttaccaaaga acaggacatt
     4741 gagcggattt ttgagtgcgg agcggacgaa tatctggcta agccctttag attcgaccaa
     4801 cttctcgaga aagtccgcga tctcataggc cgcgggcgta tcgtcgaata agctccgcaa
     4861 cccggagata tcatgagcgc ttctggcccg cgccggggaa ttgaacgcaa gatcctcaac
     4921 accatcctgc aggttgcgat cctgcccctg atcattgccg tcattgttgg ctacattacg
     4981 gcgcgggaag ggcagggaaa tgccgtcgag cagtctctcc tgaccgctgc gcgtaagacc
     5041 gccgacggac tccggatcgc ggccgctcct catctgcaga agctcgatgc cctctccaaa
     5101 gacccgaaaa tcctggcggc agtgcaaccc ggcagcagcg gtaaacctgc ggccctccag
     5161 gactccgctc aacacgaaca actccgggaa tatctcaata gccaaatcac catggtagtg
     5221 agcactcctg gggtgcccac catcctcact gtgtacgacg ctgaaggcca tctcattgca
     5281 agcacccacg aggaattgca taccctcacc agcgaagaca acgggccggg gaaatggaag
     5341 gacagccgga tcgtcaaacc gcgatttctc gacatcgatg agatcggcaa cgcctatgcg
     5401 gcagaaagcg ctgcccccat ctatcaaaac ccagaggatc gggagggact gctcggcttc
     5461 gttgctcaga ttctggacat taccccgctg ctcctcttcg cgatgggaat cgaccccaca
     5521 gacactgctg acgactcatt gcacgccgtc tatcaggttg tctacaccag cgaaggctac
     5581 catcgaacca cctacatcgc ccggaagagc gaacagggca ctttgaacct cgatgtctgc
     5641 cgtacggatt ccgcgct
//
LOCUS       DAOPBQ010000209         5653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12347_length_5653_cov_4.385852, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000209 DAOPBQ010000000
VERSION     DAOPBQ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12347_length_5653_cov_4.385852"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PKW60_07935"
     CDS             complement(<1..755)
                     /locus_tag="PKW60_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99400.1"
                     /translation="MNVDINELNALRTQLDPVMLERVDAAADHVVAAKERGGRVAVVT
                     GSGPNLHEGVTTLIAELMRLGVIDAVTTSSAVVSHEMGGTLDKVKRFQGHLVGIEAWL
                     LPRSGEFELTVMDDETLEQIARYMPVDMDLIERMRKVEGKTIIKAAGNLGYPVGLWLE
                     QLSREINELAHSCGASFEEVAGLGADERTMIGMGARKGLPVLVTVPQLIGGGCVGLHI
                     GDSISICERATRLARMLDNADVIIESAVALTQEV"
     gene            complement(783..1541)
                     /gene="kdsB"
                     /locus_tag="PKW60_07940"
     CDS             complement(783..1541)
                     /gene="kdsB"
                     /locus_tag="PKW60_07940"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPJ99401.1"
                     /translation="MSGVTIVGIIPSRYGSTRLPGKALKPILGKPMIQRVHEQVSRAR
                     GLSAVYVATDDERIADFVESFGGQAIMTSPDHPSGTDRLAEAAAQLEADIVVNIQGDQ
                     PFVDPHMIEELVQPLLDEPPLQMSTLIHALTREESLTDPSVVKVVVDREGNALYFSRS
                     LIPYPRENVPHPVYEHIGLYAYRKDFLITFTKLAPTTLELVESLEQLRALEHGCRIRT
                     VETKCADNELSGFSIDTPKDIEQAEAMLRERGLE"
     gene            complement(1613..3955)
                     /locus_tag="PKW60_07945"
     CDS             complement(1613..3955)
                     /locus_tag="PKW60_07945"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HPJ99402.1"
                     /translation="MVNAVWALLLVLQGENVATLPDTGETVERIEVANPWCRITFDPA
                     TGALRSIVNAQTGDDCLKGSQSAAMPFRLYADLTREFIIGQNEKFQLVFDDPEAIAET
                     VVQPDTCRLVEVNQEPDLTLRYEGEGLEARLRISLSEESGTSDWSLRISNTSEVTRAF
                     LVGFPYLEGVRLGPNPEHNLATAMDQAGLVVPAWERPGGVLGESNQLSMQWHAVWDPV
                     SDNALGLLFMDADVKPKRLELSEARIELHHFPPLVLAPGESVELPRVRFLVYSGDWRP
                     AARAYGAWYTHAYPHVTPPAWFRTSDGLNGLHVGVGPNRLESLRELPRLHLRTPVDVL
                     EYAFFCQGSVGHDNVHTDGDNIIREDLGGAAAMREGIAGVHRLGLHATLYVEGFIVHK
                     DSHLAATGQAQRWSVMHRDGSSTGPYSESGFLHMCPGCTEWQDHLADVVARLLTETGA
                     DGVRLDSLGFYYLPCYNPAHEHASPFDYNEWVKQLLAKVRAAAIAVKPDVLLLTEGAA
                     DWFGPWFHGALAARCPRDLPPMRLAVNPFRNYVYASGALWGSLAGYPGGGCHGSDTST
                     MDWNWACARTSAGEALVWGDVRDDGPRSPDPEIVARCFEGEGYWAVVAARPACQEAIW
                     PRGTGISDQHHPYTLTLPGLASEAQDAALCDVETLTWTPLPLEREGEDVRFELTTNWA
                     LVVLRRAGGPALAGFGSLPALTRGASVSLDTVVLLPEAADADLVAVVSAPGLHVAVEG
                     GGRVPGRATLTAPVDALPGSYAVTLSGRNLLGMKRFVVVE"
     gene            4477..5058
                     /locus_tag="PKW60_07950"
     CDS             4477..5058
                     /locus_tag="PKW60_07950"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPJ99403.1"
                     /translation="MMKQAGHPDYLVIVSGPEDGARFPIARSPVYVGRDPQCAVNLRF
                     DNTVDAYHARLAAVSSGYRVRSIGGAPVFVNGVRVGNRVSQVLRDGDFLKAGNTLFTL
                     ECAPGGLASQSRTRPGESDVAWFLRHSGDVAARITSVSARFTASALRETTRHWKFLLL
                     LALLIAYIAFSPFREGVNGLGSMALDYVRSFLP"
     gene            5308..>5653
                     /locus_tag="PKW60_07955"
     CDS             5308..>5653
                     /locus_tag="PKW60_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99404.1"
                     /translation="MKHTEARHKALEALDERLAQADSERAHDVIAYYQRAVNQTLDLL
                     ESEHPAAGLRLFQLYSSSVIVQNPETVFAIDLVSGLEEAASDLDREEGSKLRMTSNQI
                     ARFARLIDISFHT"
BASE COUNT         1075 a         1823 c         1647 g         1108 t
ORIGIN      
        1 acttcctggg tcagcgcaac ggcggattcg atgatgacgt ccgcgttgtc cagcatgcgg
       61 gccagccggg tcgcccgctc gcagatgctg atgctgtcgc cgatatggag gccaacgcag
      121 ccgccgccga tcagttgcgg cacggtcacg agcacgggca atcctttccg ggcgcccatc
      181 ccgatcatcg tgcgttcatc cgcgcccagg ccagcgactt cctcaaagga ggccccgcag
      241 gaatgggcca gctcgttgat ctcccgcgac agctgctcca gccagaggcc tacgggatag
      301 cccagattgc ccgcggcttt gatgatcgtc ttgccctcga ctttgcgcat ccgctcaatc
      361 agatccatgt ccacgggcat gtagcgggca atctgttcca gcgtctcgtc gtccatgacc
      421 gtcaattcaa attcaccgct gcgcggcagc agccacgctt cgatgccgac gagatgcccc
      481 tgaaagcgtt tgaccttgtc gagcgtccct cccatttcgt gggacacgac cgctgagctg
      541 gtggtgacag cgtctatcac gcccaggcgc atgagttccg cgatgagcgt ggtcacgcct
      601 tcgtgaaggt tcgggccgct gccggtcacc acggctacac ggccaccccg ctcttttgct
      661 gccacgacgt ggtctgccgc cgcgtccact cgctcaagca tgacgggatc cagctgggtt
      721 ctcagcgcgt tcagttcatt tatgtcaacg ttcatggcat acatcctctt tcaatatcag
      781 ccctattcca aaccacgttc ccgcaacatt gcttccgcct gttcgatgtc ttttggggtg
      841 tcaatactga atccgctcag ctcgttatcg gcgcatttcg tttcgacggt ccgtatccgg
      901 cagccgtgtt ccagggcgcg caattgttcg agcgattcaa ccagctccag cgtcgtcggc
      961 gccagtttcg taaaagtgat caagaaatcc ttgcgatagg catagagtcc gatgtgttca
     1021 tacacggggt gcggaacatt ttcacgcgga tacgggatga gtgaacgcga aaaataaagc
     1081 gcgtttccct cccgatcgac caccaccttc accacgctcg gatcagtgag cgattcttcc
     1141 cgcgtgaggg cgtgtatcag cgtggacatc tgaagcggcg gttcgtccag cagcggctgc
     1201 accagctctt caatcatgtg ggggtcgaca aatggctggt cgccctggat gttcaccacg
     1261 atatccgctt ccagctgagc cgccgcctcg gccaggcgat ccgtgccgct gggatgatcc
     1321 ggcgaggtca tgatcgcctg gccgccaaag gactcgacaa agtccgcaat ccgctcgtcg
     1381 tctgtcgcca catacacggc gctcaagccg cgcgcgcggg aaacctgctc atgcacccgc
     1441 tggatcatcg gtttgccaag aatgggcttg agcgcctttc cgggcaggcg cgtagagccg
     1501 taacgggacg ggataatgcc cacaatagtg acgccactca ttccaggtaa ctcctctttc
     1561 gggttcctgc tggaacccgg atccatcgca catgcctgcc agcggcccga actcattcca
     1621 ccacaacaaa tcgtttcatg ccaagcagat ttctgccgga cagtgtcaca gcgtaactcc
     1681 caggcagcgc gtcgacaggc gccgtgaggg tcgccctgcc cggcacgcgg ccgccgcctt
     1741 cgaccgccac gtgcagaccc ggagcagaca caacagccac caggtccgca tccgccgcct
     1801 ccggaagaag cactaccgtg tccagcgaca cggacgcgcc ccgtgtgagt gccggcaacg
     1861 atccgaatcc cgccagcgcc ggacctcccg cccgccgcag caccacgagc gcccagttcg
     1921 tagtcagctc gaaccggaca tcctcacctt cgcgttcgag cggcagcggc gtccaggtga
     1981 gtgtctccac gtcgcacagc gcggcgtcct gagcttccga ggccagtccg ggcagggtga
     2041 gcgtgtaggg atggtgttga tctgaaatcc cggtcccccg cggccagatc gcctcctgac
     2101 acgccgggcg tgccgccacc accgcccaat agccctcgcc ttcgaaacag cgggcgacaa
     2161 tctccgggtc cgggctgcgc gggccgtcat cgcgaacgtc cccccagacc agcgcctccc
     2221 cggcggaggt tcgtgcgcag gcccagttcc agtccatcgt gctggtgtcg gagccatggc
     2281 aaccgccgcc gggatatccc gccaacgagc cccatagagc accggaggca tagacatagt
     2341 ttctgaatgg attcacggcc agccgcattg ggggcaggtc ccgtgggcag cgcgcagcca
     2401 aggccccgtg aaaccacggc ccgaaccagt ccgccgcgcc ttccgttaac agcagcacgt
     2461 caggcttcac ggcgatcgcc gcggcgcgca ccttcgcgag gagttgtttg acccattcat
     2521 tgtaatcgaa aggcgaggcg tgctcatgcg ccgggttgta gcacggaaga taatagaacc
     2581 cgagcgaatc caggcggacc ccgtccgcgc ccgtctcggt cagcaggcgc gcgacgacat
     2641 ccgccagatg atcctgccac tccgtgcagc ccgggcacat atgcaggaaa ccgctctcgc
     2701 tgtaaggccc ggtggagctg ccgtccctgt gcatgaccga ccagcgctgg gcctggcccg
     2761 tcgccgccag gtggctgtcc ttatgaacaa tgaatccttc cacatacagc gtggcatgga
     2821 gtccaagccg atggacgccc gcgatgcctt cgcgcatggc tgcggcgcca cccaagtctt
     2881 cccggatgat gttgtcgccg tccgtgtgca cgttatcgtg gcccaccgaa ccctggcaga
     2941 aaaaggcgta ttccaggaca tccacgggcg tacgcaggtg gagccggggc agttcgcgca
     3001 ggctctccaa ccggttcggg ccgacgccga catggaggcc gttgaggccg tcagacgtgc
     3061 ggaaccaggc cggaggcgtc acgtgcggat aggcatgcgt ataccaggcc ccataggccc
     3121 gggccgcggg acgccagtcc cccgaataca cgaggaagcg cacgcgcggc agttccacag
     3181 attcgcccgg ggccagcaca agcggcggga aatggtgcag ctcaatccgt gcttcagaaa
     3241 gctccaggcg cttcggcttg acgtctgcgt ccatgaacaa aaggcccagg gcattgtccg
     3301 acacgggatc ccagacggcg tgccattgca tggacaattg attgctttcg cccagcaccc
     3361 cgccggggcg ctcccaggcc ggcaccacca atccggcctg atccatggca gtagccaggt
     3421 tgtgctctgg attcggcccc aggcgaacgc cctcgagata cggaaatccc acgagaaacg
     3481 cccgcgtgac ttcgctcgtg ttgctgatgc gcagagacca gtccgacgtc ccggattcct
     3541 ctgacaggct gatccggagg cgcgcctcca ggccttcccc ttcatagcgc aaggtcaggt
     3601 caggctcctg gttaacctcg accaggcggc acgtatcggg ttgcaccacc gtctccgcta
     3661 tggcctccgg gtcgtcgaag accagttgaa acttctcgtt ctgcccgatg atgaactcgc
     3721 gagtgagatc cgcatacaag cgaaagggca tcgcagcgga ttgggaccct ttgaggcagt
     3781 catcccccgt ctgcgcgttg acgatgctgc gaagcgcgcc ggtggccggg tcaaacgtta
     3841 tccggcacca tggattcgcg acttcgatcc tctcgaccgt ttcccctgtg tccggcagcg
     3901 tcgcgacatt ctccccctgc aggaccagca gcaacgccca taccgcattt accatgaacg
     3961 gtctccgcct ctcaacagaa aacacaaaac ggcggccaat tctcccgccg ttcgccggtg
     4021 atcctacgcg cagcggattt accatagcac aagatatgaa cgttcacaag tgaacgttca
     4081 cattcccgtc catgcacggt tttgggcgtc cgtgaggcga gttactgagc atcgtcaccc
     4141 cccaagaagc gttacgtaaa gcgaagcacg tgcagcatct gggggtagaa cgagtcccag
     4201 gcggattcca ggcgcttttc cgacgtgcgg tgcgcgctcc gtgtgtgtcc gaattgaggc
     4261 tgattgttgg catcccatgc gccatattgg tagagttgcg cgcatacggt tgtgaggctg
     4321 ttttggattt gaacctcagt tgggcaggag ctcacccgta tcctggccga ggcgggtgcc
     4381 gttactgttc gcacgaggca acgcagttcc aacatcgagg atacgtcaga gattttttct
     4441 caaaggcaag tgcaccattt ccataaaggt cccccaatga tgaaacaggc cggccatccc
     4501 gattatctgg tgattgtgag cgggccggaa gatggcgcga ggttccccat cgcccgctcg
     4561 cccgtctatg ttgggcgaga tccgcaatgc gccgttaatt tgcggtttga caataccgtt
     4621 gacgcgtatc acgctcgttt ggcggcagta tccagtgggt atcgtgtccg cagcattggt
     4681 ggcgcgcccg tgtttgtgaa cggagttcgg gtcggtaacc gggtttcaca agtcctgcgc
     4741 gacggcgatt tccttaaagc cgggaacacc ctctttacgc tcgaatgcgc tccgggcggc
     4801 ctggcgagcc agagccgcac caggcccggt gagagcgatg tggcctggtt tctgcgccat
     4861 tcaggtgatg ttgcagcccg gattacgagc gtttcggccc gatttacggc cagcgccttg
     4921 cgcgaaacga cccgtcactg gaaattcctg ctcctcctcg ctcttctcat cgcatatatc
     4981 gccttttcgc ccttccgcga aggtgtgaac ggcctcggca gcatggcttt ggattacgta
     5041 cggtcgttcc tgccgtgagg catttcgagt ggcgcaatct tacgccttat cccatccaca
     5101 ctgtggacag cgcccggggc aagctgctac aacagatttt cagtgtgcaa caggccttta
     5161 tattggctcg cggggcaaca taggaaaccg ggaggtttgt atatggcgag gcgtttgact
     5221 gcggctatgg cgttatgcat gtgttgggga acgtttgcgc aagatctgaa cgctgtttgg
     5281 caggggattc tcgcaaaccc gccggcattg aagcatacgg aagcgcgcca caaagccctg
     5341 gaagccctgg atgagcgcct cgcgcaggcc gattcggagc gtgcgcacga cgtaatcgcg
     5401 tattaccagc gtgcggtaaa ccagacgctc gatctcctcg aatccgaaca tccggccgcg
     5461 gggctccgcc tgtttcaact ctattcaagc tccgttattg tgcagaatcc ggagaccgtg
     5521 ttcgccatcg atctcgtttc tggccttgag gaggccgcat ccgacttgga tcgtgaagaa
     5581 ggcagcaagc tcaggatgac ctccaatcaa attgcgcgat tcgcccggct gatcgacatt
     5641 tcctttcaca ctc
//
LOCUS       DAOPBQ010000210         5647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12361_length_5647_cov_4.522175, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000210 DAOPBQ010000000
VERSION     DAOPBQ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12361_length_5647_cov_4.522175"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PKW60_07960"
     CDS             complement(<1..402)
                     /locus_tag="PKW60_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPJ99405.1"
                     /translation="MLLGEILYPDVVKTTLEAENKYEAIDELVDLLVENHEIPLALRE
                     HIVEVIVEREKSLSTGMEYGIALPHGSTDAVDDIIGALGISRRGLPFESLDGKPAHIV
                     ILLVLPKSKFQAHVRTLGGIAHLMSNASLRQA"
     gene            complement(406..1062)
                     /locus_tag="PKW60_07965"
     CDS             complement(406..1062)
                     /locus_tag="PKW60_07965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPJ99406.1"
                     /translation="MSAEHTAPTRMNVLARRAQIRLASDGVVLLKGKREALLKELLVR
                     ARKLRKLRHELHRRGRAAVAALAVARATSGAPEVRSAAIAGQRRVPLTVKSEKVWGLE
                     LYDIEHEPIVRSPGERGTGVLTTSSQILDAAETAEALLERLVECAPIESNIQVLGDEV
                     RKVSRRISALDDHLLPRLRDDLRRISRALEEREREDVFRLKRIKQKKAAARRNAAGDP
                     "
     gene            complement(1066..2514)
                     /locus_tag="PKW60_07970"
     CDS             complement(1066..2514)
                     /locus_tag="PKW60_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPJ99407.1"
                     /translation="MQTHDEQRHGTTRPDLVHREYWNITQVTGPLVFLGNGDRFPTGA
                     ILDLVLESGERRQGQVLEASSERAVVQVLQGTQGVDVKRTAVSLRQEAARIVASKDVI
                     GRRFNGVGAPIDGLPPLADGVELDIAGAAINPVSRDNPSDFIETGISTIDGFNTLVRG
                     QKLPIFFGAGLPANELASLIVRQAATRNDAGEFVVVFGSMGITEREAAFFLRSFEEGG
                     RGSRVVAFINKASDPAIERLFTPRCALTLAEYLAFEHDFHVLVVLSDMTNYCEALRQI
                     ASAREEIPGRRGYPGYMYTDLSSIYERAGRIRGKKGSVTQIPMLSMPDDDITHPIPDL
                     TGYITEGQIVLSRALHRRGVFPPVDVLPSLSRLMNNGIGDGLTRADHRELANQLYACY
                     AHGRDIRKLMSIVGEDALTELDQKYLRFAEGFEAQMVGQGHSRRAIDETLGLGWQLLN
                     ILPKEELTRINKDLLDRYYSQTMEDGVPSPYI"
     gene            complement(2520..4298)
                     /locus_tag="PKW60_07975"
     CDS             complement(2520..4298)
                     /locus_tag="PKW60_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPJ99408.1"
                     /translation="MALSEHPIVGNLTQISGPMVAADGMLGVAMGEIVRVGKAQLLGE
                     VIRIDGATVYAQVFEDTGGLYVGEPAEAMGVALSVELGPGLLGGTFDGIQRPLESLRQ
                     VSGDFIARGLASHALDRDAKWHFVPRVTPGTEITGGMVLGTVQETASIEHRILVPPET
                     SGRVARVAPEGEYTIVEPVVELDDGATLTLMHRWPVKQPRPVERKLPPDRPFLTGQRV
                     LDCLFPIALGGAAIVPGGFGTGKTVVEQTLAKFCNAEVIVYVGCGERGNEMAGVLSEF
                     PGLKDPRTGESLMNRTVLVVNTSNMPVAARDASVYTGITIAEYYRDQGYDVAVMADST
                     SRWAEALREISSRLEEMPGEEGYPTYLSARIAEFYERSGRVVCSGSADDERVGSLTLV
                     GAVSPPGGDFSEPVTQTSLRVTGGLWALDPTLAYRRHYPAINWSRSYSLYFNELNAWF
                     EEHAPAGWNRQRQAAMHLLQRDAELQEVVQLVGPDALQDSERLVLEISKMIREIFLQQ
                     NAFSDSDAFSSLEKTAGLLDALLAFHDACRKKLENGVTLVRILDLPVREDLARLRDVP
                     GEAFMERQHEVVEAMKTALAELTPKG"
     gene            complement(4356..4625)
                     /locus_tag="PKW60_07980"
     CDS             complement(4356..4625)
                     /locus_tag="PKW60_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99409.1"
                     /translation="MPEAVGIADEHLAGLLALTGMSVEEVRDAAEAQEALDRLLDSGA
                     QCIIVQESFREAFSPWFNDRLLAHRGLPLVMYCPAFDQEDAEIDA"
     gene            complement(4618..>5647)
                     /locus_tag="PKW60_07985"
     CDS             complement(4618..>5647)
                     /locus_tag="PKW60_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPJ99410.1"
                     /translation="VYIATRMRGMKSRLLSRDALEDLLDHKELDALIEVLLATDYRDE
                     MARALAVREGADAIEEAVSRHLMLTFAQLLTISGNWYRPYTALFLTRWDIQTAKQLLR
                     QRLARDRGITATGFPAPGPSLGVALVRHLAELDSLEALLQGLMSWNRPLCACLWKVYL
                     DGGAEPLLRAMEDALDRAYFVEGARRLAQDPDENAQILCQYLRMSIDGINLRVLLHGK
                     AHQDAHADWLLPEGTIPKRLAAAMMAAENVEEAVALLESTIYRGLAEGLYVFLQTGRY
                     SPMQRMFEQLLVRELRKLARTRAMTLAPLMHYAWLKYNEVINLRLIARGELRQLPRGR
                     IREEMIHA"
BASE COUNT          970 a         1728 c         1906 g         1043 t
ORIGIN      
        1 ggcctggcgg aggctggcgt tgctcatgag gtgggcaatg ccgccgaggg tgcgcacgtg
       61 cgcctgaaat ttgcttttgg gcaggacaag caggatgacg atgtgggcgg gtttgccgtc
      121 gaggctttcg aaggggagtc cgcggcgcga gatgcccagg gcgccgatga tgtcatcgac
      181 ggcgtcggtg gagccgtgcg ggagcgcgat gccgtattcc atgccggtgc tgagggattt
      241 ttcccgttcg acgatcacct cgacgatgtg ctcgcgcaag gcgaggggga tttcatggtt
      301 ttcgacgagc agatcgacca gctcgtcgat ggcttcgtat ttgttctcgg cttcgagcgt
      361 ggttttcacg acgtcggggt agaggatttc gcctaacagc atggcttacg gatctccggc
      421 ggcgttgcgt cgtgccgcgg cttttttttg tttgattcgc tttagacgaa acacgtcttc
      481 gcgttcgcgc tcctcgagtg cgcggctgat tcgacgcaga tcgtcgcgca atcggggcag
      541 gaggtggtcg tcaagggcgc tgatgcggcg gctgactttg cgcacttcgt cgcccagcac
      601 ctggatgttg gattcgatgg gcgcacattc cacgaggcgt tcgaggaggg cctcggcggt
      661 ctcggcggcg tcgaggatct gcgacgatgt ggtcagcacg ccggtaccgc gttcgccggg
      721 gctccgcacg atgggctcgt gctcgatgtc gtacagttcg aggccccaga ctttctccga
      781 tttgacagtc agcgggaccc ggcgctgccc ggcgatggcg gcggagcgga cttcgggggc
      841 gccgctggtc gcgcgcgcga cggcgagtgc cgcgacggcg gcgcggccgc ggcggtgcag
      901 ctcatgacgc agcttccgga gcttccgggc gcgcaccagc agctctttga gcagggcctc
      961 gcgctttcct ttgagcagca caaccccgtc ggacgcgagc cggatctggg cgcgtctcgc
     1021 cagtacgttc atccgggttg gtgctgtatg ttcggcgctc atgggctaga tgtacggcga
     1081 cggcacgccg tcctccatgg tctgtgaata gtaccggtcg agcagatctt tgttgatgcg
     1141 cgtcaactcc tctttcggga ggatgttcag gagttgccag cccaggccga gcgtctcgtc
     1201 gatggccctg cgggaatggc cctggcccac catctgggcc tcgaatcctt ccgcgaaacg
     1261 gaggtatttc tggtcgagct cggtcagggc gtcctcgccg acgatcgaca tgagtttgcg
     1321 gatgtcgcgt ccgtgggcgt agcacgcgta caactggtta gccagttcgc ggtggtcggc
     1381 ccgggtcagc ccgtcgccga tcccgttgtt catcagccgg gacaggctgg gcaggacgtc
     1441 gacgggtgga aacacgccgc ggcggtgcag tgcgcggctc agaacgatct ggccctcggt
     1501 gatgtaaccg gtcagatcgg gaatgggatg ggtgatgtcg tcgtcgggca tggagagcat
     1561 ggggatctgg gtgaccgaac cttttttgcc gcggatgcgc ccggcccgtt cgtaaatcga
     1621 ggacaggtcg gtgtacatgt agccgggata accccgccgg ccggggatct cttcacgggc
     1681 gctggcgatc tggcgcaatg cttcgcagta attcgtcatg tcggagagga ccaccaggac
     1741 atggaagtcg tgctcgaacg ccaggtattc cgccagggtg agcgcgcacc gcggcgtaaa
     1801 caggcgctcg atggcggggt cgctggcctt gttgatgaac gcgaccacgc gcgagccgcg
     1861 cccgccctct tcgaacgaac ggaggaaaaa cgccgcttcg cgctcggtga tgcccatgga
     1921 cccgaatacc accacaaatt ccccggcatc gttccgggtt gcggcctggc gcacgatcag
     1981 gctggccagt tcgttggccg gcagaccggc cccgaaaaag atggggagtt tctgtccccg
     2041 gaccaacgtg ttgaaaccat cgatcgtgga gatgccggtt tcgatgaaat cgctgggatt
     2101 atcgcgggaa acggggttga tggccgcgcc ggcaatgtcg agttcgaccc cgtccgccag
     2161 aggcgggagc ccgtcgatcg gagcgcccac cccgttaaac cggcggccga tgacatcttt
     2221 cgacgccaca atgcgggcgg cctcctggcg cagggagacg gcggtgcgtt tgacatcaac
     2281 gccctgcgtt ccctgaagta cctggacgac ggcgcgctcg gaactggcct cgaggacctg
     2341 gccctggcgg cgttcgcccg attcgagcac gaggtcgaga atggcgccgg tgggaaagcg
     2401 gtccccgttg ccgagaaaca cgagcgggcc ggtgacctgc gtgatgttcc agtattcgcg
     2461 gtggacaagg tcggggcgcg tggtcccgtg gcgctgctca tcgtgtgtct gcatggcgct
     2521 tatcctttcg gagtcaattc cgcgagggcg gttttcatgg cctcgaccac ctcgtgctgg
     2581 cgctccataa aggcctcgcc cggcacgtca cgcaggcggg cgaggtcttc gcggacaggc
     2641 agatcgagga tgcggaccag ggtgaccccg ttctcaagct ttttgcggca tgcgtcgtga
     2701 aacgcgagca gcgcatcgag gagtccggcg gttttttcga gtgaggagaa ggcgtcgctg
     2761 tcggaaaagg cgttttgctg cagaaagatc tcgcggatca ttttcgagat ctcgagcacc
     2821 aggcgttcgc tgtcctgcag cgcatcgggt ccgacgagct ggacgacctc ctgcagctcg
     2881 gcgtcgcgtt gcagcaggtg catggcggcc tgccgctggc ggttccaacc ggccggcgca
     2941 tgttcctcga accaggcgtt aagctcgttg aaatagagcg aatagctgcg gctccagttg
     3001 atggcggggt aatggcggcg gtaggccagc gttgggtcga gcgcccagag gccgcccgtc
     3061 acccgcaggg aggtctgcgt gaccggctcg gagaagtcgc cgcccggcgg cgagaccgcg
     3121 ccgaccaggg tcagagagcc cacgcgctcg tcatccgcgc tgccgctgca gacgacacgt
     3181 ccgctgcgtt cgtagaattc ggcgatccgg gctgagaggt aggtgggata gccttcttca
     3241 ccgggcatct cttcgaggcg cgaagagatc tcgcgcaggg cctccgccca gcgtgaggtg
     3301 ctgtccgcca tcaccgccac atcatagccc tggtcgcggt aatactcggc gatcgtgatg
     3361 ccggtataga cggaggcgtc gcgcgcggcc acgggcatgt tcgacgtgtt caccacaagc
     3421 acggtacggt tcatgagcga ctcgccggtg cgcgggtcct tcaatccagg aaattccgac
     3481 agcacgccgg ccatctcatt cccccgctcg ccgcacccga catagacgat gacctcggca
     3541 ttgcagaatt tggcgagggt ctgttcgacc acggttttcc cggtgccgaa gccgcccggg
     3601 acaattgcag cgccgcccag ggcgattgga aagaggcagt cgagcacccg ttgcccggtc
     3661 aggaaaggcc ggtcgggagg aagcttgcgc tccacggggc ggggctgttt cacgggccac
     3721 cggtgcatca gggtcagcgt ggcgccatcg tccagttcaa caacgggctc aacaatcgta
     3781 tactcgccct cgggtgcaac ccgggccacg cgcccggaag tctccggcgg aaccaggatg
     3841 cggtgttcga tgctggccgt ttcctgaacg gttccgagga ccatgccgcc cgtgatctcg
     3901 gtgccaggcg tcacacgcgg cacaaaatgc catttcgcat cgcggtccag ggcatgcgag
     3961 gccagtccgc gcgcgatgaa atcgcccgat acctggcgca acgattcgag aggccgctga
     4021 atgccgtcga aggtgccgcc gaggagtccc gggcccagtt cgaccgacag ggcaaccccc
     4081 atcgcttcag caggttcgcc aacgtagagg ccgccggtgt cttcaaaaac ctgagcatag
     4141 accgtggcgc cgtcgatccg gatcacctcg cccaggagtt gagccttgcc gacgcggacg
     4201 atctcgccca tggccacgcc cagcatgccg tccgcggcca ccatcggccc ggatatctgc
     4261 gtgaggtttc caacaatggg atgttccgac agggccatat gcctctactc cagacggatc
     4321 tcgtacccga ccgccggccg gatcaccgac gtgagttacg catcgatttc cgcgtcctcc
     4381 tggtcgaacg cggggcagta catcacaagc ggcaggccgc ggtgcgccag caggcggtcg
     4441 ttgaaccacg gcgagaaggc ttcccgaaaa ctctcctgca cgatgatgca ctgagcgccg
     4501 ctgtcgagca gccgatcgag ggcctcctgc gcctcggcgg catcgcgcac ttcttcaaca
     4561 gacatccccg tgagggccag caggcccgcg agatgttcat cggcgatccc cacggcctca
     4621 ggcatggatc atctcctcgc gaatccgccc gcggggcaat tggcggagct caccgcgcgc
     4681 gatcaggcgg agattaatga cttcgttgta tttcagccag gcgtagtgca tgagcggtgc
     4741 gagcgtcatg gcgcgcgtgc gcgccagttt acgcagctcc ctcaccagga gctgctcgaa
     4801 catgcgctgc atcggagaat agcggcccgt ctgaaggaag acatacaagc cttcagccag
     4861 gcctcggtag atggtcgatt ccaggagcgc cacggcttct tcaacgttct ctgcggccat
     4921 catggcggcg gcgaggcgtt tcggtatcgt gccctcgggc agcaaccaat ctgcatgggc
     4981 gtcctggtgg gccttgccgt ggagcagaac acgcaggttg atgccatcga tgctcatgcg
     5041 gagatactgg cacaagattt gggcgttttc atcgggatcc tgcgcgagac gccgggcacc
     5101 ctccacgaaa tacgcccgat ccagggcgtc ttccatcgcg cgcagcagcg gctcggctcc
     5161 tccgtcaagg tataccttcc agagacacgc gcaaagcggg cggttccacg acatcaggcc
     5221 ctgcagcagc gcctccaggg aatccagttc cgccagatgc ctgacaagcg cgacgcccag
     5281 ggaagggccc ggggccggaa acccggtcgc ggtaatgccg cggtcccggg ccaggcgctg
     5341 gcggaggagc tgcttggcgg tctggatatc ccaccgggtc agaaacaggg cggtgtacgg
     5401 ccggtaccag ttgcccgaaa tggtcagaag ctgcgcaaag gtcagcatga gatgccggga
     5461 gacggcctct tcaatggcgt ccgcgccttc gcgcacggcg agcgcccgtg ccatctcgtc
     5521 tctgtaatcg gtagccagca agacctcgat caatgcgtcg agttctttgt gatcgagcag
     5581 atcttcaagg gcgtcgcgag acagcagacg gcttttcatg ccgcgcatgc gggtggcgat
     5641 gtagacg
//
LOCUS       DAOPBQ010000211         5615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12437_length_5615_cov_3.072302, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000211 DAOPBQ010000000
VERSION     DAOPBQ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12437_length_5615_cov_3.072302"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            667..1593
                     /locus_tag="PKW60_07990"
     CDS             667..1593
                     /locus_tag="PKW60_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99411.1"
                     /translation="MKKRWVITAAVLLVVIGVIVVVLADRKYCLFPSPKFSHAPYVTP
                     DTRVQIGLWPGNGGEDLLAILRAALPEKQRPSNWLLKRILPHEATVLLAPNLQSQSIA
                     VTAFINDQRFGPVIRDAVNEANPSRSVPVVRWLADGMVVEKRGILTLRGDIPMDAHST
                     QLTQSSWPGAPAGAPLALEGGHAIEIMLDNRDGGAFAVLSSLELLKGLDGENNPDFYV
                     ELLANVSWLRLFADFAGTNDAALKLHIECRPDAEPGVAGSIQFVVDMGLMAAKKALEA
                     KGAALVGASRVEGNAVVGDYTLRGLDKLIPAG"
     gene            complement(1652..2251)
                     /gene="recR"
                     /locus_tag="PKW60_07995"
     CDS             complement(1652..2251)
                     /gene="recR"
                     /locus_tag="PKW60_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPJ99412.1"
                     /translation="MLFNTPAFEQLVDALRRLPGVGKRSAERLALHLLSAPAEEAQTL
                     SEALVEARRCITRCSECCNLTDQDPCAICAHPNRDHSVICVVEHPSGAMAIEKAGAYK
                     GLYHVLHGALSPLEGIGPDELTIDALLRRIPGKVREVIVATNATAEGEATALYLSRVI
                     AQRFDTQVTRIAHGVPMGSGLEYADDATLTHALEGRKQL"
     gene            complement(2300..2806)
                     /gene="def"
                     /locus_tag="PKW60_08000"
     CDS             complement(2300..2806)
                     /gene="def"
                     /locus_tag="PKW60_08000"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPJ99413.1"
                     /translation="MNIVLYPDDPLLSVAAPVERFGSHLEKLAVTMLETMYEYEGVGL
                     AAPQVGISKRFFVCHDLESDPMCLVNPEIVEREGEEIADEGCLSIPRIYAPVPRATHI
                     RVRAQDLQGNDLDFVAEGFLARIIQHETDHLDGRVFLDRVDILTRQSKLVEWEEVRQS
                     LESAAPLG"
     gene            complement(2966..5569)
                     /locus_tag="PKW60_08005"
     CDS             complement(2966..5569)
                     /locus_tag="PKW60_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99414.1"
                     /translation="MPRSRRTFFVYARACLALLLAGAAVTGMAATEPSTGAADVRVWA
                     YPSRYQDGAHVKASFQLDEVTPVEEALTIPCEIQISVALDQPQARLHVYDEQGNALFE
                     GRAELHGARSASQPVFAWPLEGVGDGVYRARLEVLHALNQPLAWREIELEKRSYGALL
                     QRFGHVHALLGPVQESLREARPAEVPLYASIRAAIAEDALDQAEVFLQEGDWPHATAL
                     SEFAAMSCDSARALLTFAPLTPELARPGPVPSATSLRIRDGAFYTPASDPVFLFGFRD
                     PDINPRTLALLDRYGLNFAAIDVSPADLLLEGGTNLDTVARVDTYLENADALGIGVTV
                     GLAANVAPAWLRSGQTAMTPDGSEKTAVPGPGEARALSQRHLDAVAPLLQRHPSVNGV
                     SLASAPAFHWETEEVRQGFQQAMALKYKEDRRAVNRAWRTRLRALDEIQILWDYHQPS
                     YQYDWQTWHQHLVSNHFTWLAGCARRALPNLPLQIVLPGTAAQTGEARTGIDRQALVR
                     LTDVTGCAASYAPGSSIYAVPFAGPELSFRLLRSLAPANPLVVSALTFDLSQIPAGAR
                     ASQHVHAALWDAAIAGVNGFAARPSEFEQNPGGLEGFATACADLNRLGAIVTAFQRSP
                     APIAILWSAPSKIYLDGETFLESVQRVYEGSAFFGFPIRFITEEACISGGLADVSVLA
                     MPEVPALADGTFQAINEYAKAGGAVIRSGRPAPYTPAGVSRHDVITQTSRTIFVRGSD
                     TPTVYLHALDAAYVFGVLPPIPRTINSYGYPLEGVKSRYVELDGQEYLYVVNLRKEPA
                     RVNLYGGPRKGRDLVRGRSIQFPTVLEPLEPVLVHLAPVGSATPIPGDAPVLAENQGE
                     PGPVVLSPVTP"
BASE COUNT         1084 a         1689 c         1814 g         1028 t
ORIGIN      
        1 caacgatgac cgggcctgtc aagccatttg cttggctccg cacggagaat ccacccccaa
       61 atcggctgcc gccgattcac ccccgccagc gggggacagg tgcaccgcgc ataagtctct
      121 ggttttaatg cccttatgtc cttcgaagaa gaggacggat caaaaaccaa accaagcgcc
      181 gcgtatgccg gttcgcattc ttgtgtcccc cgcgggcggg ggtggacacg cgccccgcgt
      241 ggccgggggt ggacccagtg gacccaatgg acagggtgga ccgggccccg acagcggagc
      301 catcgcccgt gccggccctt gtattccccg ctgacggagc aacgatgtat gcttcgccgc
      361 caccgccggt tcgcgttctt gtgtcccccg cgggcggggg tggacacgcg ccccgcgtgg
      421 ccgggggtgg actctcgtct ttcatccccg aaacgtgttc aatccatcca aaatccgttc
      481 tcctgacatc gtcttgcctt ccatatttcg gtaagatcgc aaatgacagg acgataccat
      541 ccccggtaag atctcagatg gcgcgattcg tcaggttgac ttaaccggcc cgggcgcata
      601 cgatctcgag tctattgtgt gtctcgtgca aaaatggggc gctgattcga aagggagtga
      661 tgaattttga agaaacgctg ggtgataacg gcggcggttc tattggttgt tattggcgtg
      721 atcgttgtgg tgctggccga tagaaaatac tgcctgtttc caagccccaa attctcacat
      781 gcgccttacg tgaccccgga tacgcgggtg cagattggac tctggcctgg aaacggcggg
      841 gaagacctgc tggccattct ccgggcggct ttgcctgaaa agcagcgccc gagcaactgg
      901 ctgctgaagc ggatcctgcc ccatgaagcc acggtactcc tcgctcccaa tctccagtcg
      961 caatccatcg cggtgacggc gtttattaac gaccagcgct tcgggccggt aattcgtgat
     1021 gcggtcaatg aggccaaccc ctcccgttca gtgcctgtcg tccggtggtt agcggatggc
     1081 atggtggtgg agaagcgcgg aatcctgact ttgcgcggcg atatccccat ggacgcgcat
     1141 tccacccagc tcacgcaatc cagctggccc ggggcgccgg cgggcgctcc cctggcgctg
     1201 gagggcggcc acgcgatcga aatcatgctc gataaccggg atggcggggc ttttgccgta
     1261 ttgtcgtcgc tggaactgct gaaagggctc gatggagaga acaatccgga tttttatgtg
     1321 gaattgctgg ccaacgtgtc gtggctgcgg ctcttcgccg atttcgcggg cacgaacgac
     1381 gccgcactga aattgcatat cgagtgccgg cccgatgcgg agcccggtgt cgcgggcagc
     1441 atacaatttg ttgttgatat gggccttatg gcggccaaaa aggcactgga agccaaggga
     1501 gctgctcttg ttggggccag ccgcgtcgaa ggcaacgccg tggtcggcga ctatacgctt
     1561 cgcggcctgg acaagctgat tccggcgggc tagagagcgg tctccgcggc attagagcag
     1621 agctgagcca ggttccggcg ggcagccggg gtcagagctg tttgcgccct tccagggcat
     1681 gggtcagggt agcatcatct gcatactcga gcccgctccc catgggcacg ccgtgggcaa
     1741 tacgcgttac ctgtgtgtcg aaccgttgcg cgatgacccg ggagagatac aaggccgttg
     1801 cctccccttc cgcggtggcg ttcgtcgcca caatgacttc ccgaaccttg cccgggatcc
     1861 ggcggagaag cgcatcaatg gtgagctcgt cggggccgat tccctccagt gggctcagtg
     1921 cgccgtggag cacgtggtaa aggcccttgt acgcgccggc cttctcaatg gccatggcgc
     1981 ctgagggatg ttcgactacg cagatgacac tgtgatcccg gttgggatga gcgcagatcg
     2041 cacacggatc ctggtcggtg agattgcagc attcagaaca gcgcgtaatg cagcgccggg
     2101 cctccacgag ggcctcgctc agggtttggg cctcttccgc gggggcggac aggagatgca
     2161 gcgccagtcg ttccgcggac cgttttccta ctcctggcag gcggcgcaga gcatcgacga
     2221 gttgttcaaa agcaggtgtg ttgaacagca tggttccccc gaatgcctcg tgaggcggaa
     2281 aggtgggccg gccgggggct cagcccaacg gggccgcgga ctccagggat tggcgaacct
     2341 cttcccattc gacgagcttc gactgccgtg tcaggatatc gacccggtcc agaaacaccc
     2401 gcccatccag gtgatcggtc tcgtgttgaa tgatacgtgc caggaacccc tccgccacga
     2461 agtcgaggtc attgccctgg agatcctggg cgcgcacacg aatgtgcgtc gctcgcggca
     2521 cgggcgcgta gatgcggggg atgctcaggc atccttcgtc ggcgatttct tcaccttccc
     2581 gttccacgat ctcgggattg accaggcaca tgggatcgga ttcgaggtcg tggcaaacga
     2641 aaaatcgttt gctgataccc acttgcggcg cagccaggcc cacgccttcg tactcgtaca
     2701 tcgtctcgag catcgtgacg gccagttttt cgagatgcga gccgaaacgc tcaaccggcg
     2761 ccgccacgct cagcagcggg tcgtcgggat agagaacgat attcatactg cccaaactcc
     2821 ttcatccccg tgatgttagc acatgcgcac gacgggcaca aacgcccacg cgagcctggg
     2881 aataatcctc aaagacaaca cgccggcgca acgagataat ccggtccggg attcgtaccg
     2941 caagactggt ttccacggca gggtttcaag gggttacggg gctcaggaca accggaccgg
     3001 gttcgccctg gttttcagcc agcaccggcg catcgcccgg gatcggcgtg gcggagccca
     3061 cgggagccag atggacaagg acgggttcca gcggctccag caccgtcgga aattgaatgc
     3121 tgcggccgcg cacgagatcc cgtcctttgc gcgggccgcc atacaggttg acccgcgccg
     3181 gttctttccg aaggttgacc acgtacagat attcctgccc atccagctcg acataccggc
     3241 tcttgacccc ctccaaaggg tagccatagc tgttaatcgt ccgggggata ggcggcagaa
     3301 cgccgaacac ataggctgcg tcgagcgcgt gcaggtagac ggtgggtgta tcggaacccc
     3361 ggacaaaaat ggtgcgcgag gtctgtgtaa tcacatcgtg acgcgaaacg ccggcgggag
     3421 tgtacggtgc ggggcgcccg ctgcgaatga cagcgccgcc ggccttggcg tattcgttga
     3481 tcgcttgaaa cgtcccgtcg gccagtgcgg gcacctccgg catggccagc acggacacat
     3541 cggccagccc gcccgaaata cacgcctctt cagtaataaa acggattgga aagccgaaaa
     3601 atgcgctccc ctcatagaca cgctgaacgg attccagaaa cgtctcgccg tccagataaa
     3661 tttttgaggg cgcgctccac aggatcgcga tcggagccgg cgagcgttga aaggcagtga
     3721 ctatggcgcc caggcggttc aggtccgcac aggccgttgc gaagccttcg aggccgcccg
     3781 gattctgctc aaactcggat ggacgggcgg caaaaccgtt cacacccgca atggctgcgt
     3841 cccagagggc ggcatggaca tgctgggagg cgcgggcgcc ggcgggtatc tggctcagat
     3901 cgaacgtgag agccgagact acaagagggt tggcaggggc cagtgaacga agcaatctga
     3961 acgaaagctc ggggccggca aacggcaccg catatattga actgccggga gcgtaggatg
     4021 cggcgcaccc ggtcacatcg gtcagacgga cgagggcctg gcggtcgata cccgtgcggg
     4081 cctcgcctgt ctgggcggct gtgccgggca ggacaatctg gagggggagg ttagggaggg
     4141 cccggcgggc gcagccggcc agccatgtga aatggttcga gaccagatgt tgatgccagg
     4201 tttgccaatc gtattggtac gagggctggt ggtaatccca gaggatctgg atttcatcca
     4261 gcgcgcgcag acgggtccgc caggcgcggt taacagctcg ccggtcttcc ttatatttaa
     4321 gggccatggc ctgctgaaaa ccctgccgca cttcttccgt ttcccagtgg aatgcggggg
     4381 cagacgccag actgacgccg ttgacggaag gatgccgctg gaggagcggg gctactgcgt
     4441 cgaggtgccg ttggctgagc gctcgcgctt cgccaggccc cgggactgca gtcttctcgg
     4501 aaccatccgg ggtcatggcg gtctgcccgg agcgcagcca cgcgggggcg acatttgcgg
     4561 ccaggccgac cgtcacacca atgccgaggg catccgcgtt ctcgaggtag gtatcgacac
     4621 gcgccacggt atccaggttg gttccgcctt cgagcaggag atccgcggga gatacgtcga
     4681 tggcggcaaa attgagcccg taccggtcga gcagggccag ggtccgcggg ttgatatcgg
     4741 gatcacgaaa tccaaacaag aacacagggt cagatgcggg ggtgtaaaag gccccgtcac
     4801 ggattctcag ggacgtggcg gaaggcaccg gccccggcct ggccagctcg ggagtcaacg
     4861 gcgcgaaggt cagcagggct cgcgcagaat cgcacgacat cgctgcgaac tcactcagcg
     4921 cggtggcgtg cggccagtcg ccttcctgaa gaaatacctc cgcctgatcc agggcatcct
     4981 cggctatcgc cgcacgaata gacgcataaa ggggcacttc cgccgggcgc gcttcccgaa
     5041 ggctctcctg tacggggccg aggagcgcgt gtacgtgccc gaagcgctgc aaaagcgctc
     5101 cataagatcg tttttcgagt tcaatttcgc gccaggccag aggctggttg agcgcatgca
     5161 agacctcgag gcgcgcgcga tagaccccgt cgcccacgcc ctccagaggc cacgcgaaca
     5221 ccggctggga cgcgctccgg gccccgtgca gctctgcccg gccttcgaac agtgcgtttc
     5281 cctgctcgtc gtacacgtgc aggcgcgcct gtggctggtc cagcgcgacg ctgatctgta
     5341 tttcgcaggg aatcgtcaac gcctcttcga cgggtgtgac ctcgtccaat tgaaacgacg
     5401 ctttgacgtg cgcgccatcc tggtaccggg aggggtatgc ccagacccgt acatcagcag
     5461 cgccggtgga cggctctgta gcggccatcc cggtcacggc cgcgccagcg aggagcagtg
     5521 cgaggcaggc ccgggcgtat acaaaaaacg ttcggcggga acgcggcata gattcatcct
     5581 cttcggaacg atacacagaa ccgggttgat tgaat
//
LOCUS       DAOPBQ010000212         5606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12454_length_5606_cov_3.563682, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000212 DAOPBQ010000000
VERSION     DAOPBQ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12454_length_5606_cov_3.563682"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1026
                     /gene="flgA"
                     /locus_tag="PKW60_08010"
     CDS             55..1026
                     /gene="flgA"
                     /locus_tag="PKW60_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HPJ99415.1"
                     /translation="MNREQTPHRLLFAWAAAAVLFLAAVRADALNVVLLRDEAFVKGP
                     QVALGDVADIEGAEAEALAGLEILPAAAPGCVKRIHASMLRSRIMTAGYRPEDFEITG
                     AESVNATTLCVELTREMLVDDLRRFIELEMPWDPANATVDIDPPTSKLVLPEGDIVLE
                     WRPAALFQYLGKGTMRGEALVDGQVRDVIYCQVNVQAYGDVVVASSDIPRGAIISRGD
                     LSVAKRALSAMRNGYYSDPEELAGMVARSTIFPDTVLTSRHVMPRRIIKRNQVVTVEV
                     RAGTLMVRDRAQAMSNAAAGDIIICQRMNSKEEFQGIVREDGIVEVY"
     gene            1044..1682
                     /locus_tag="PKW60_08015"
     CDS             1044..1682
                     /locus_tag="PKW60_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HPJ99416.1"
                     /translation="MKKTYLVAVLLAAVLAVPVRADSLFSKGVAKRGPAVSNKHTRFT
                     EGDIIIVLVRENIDATTRANTNTKKESELIAEAPINSNTFLVAPKPDGLGIVKPERLP
                     VWDINVENEHKGRGDTSRTNKLTTTISCIVTKVHDNGNLEIEGNKRVTVNREDSTILV
                     TGTVRARDVSPENTVPSDRVANAIVELKGKGPLWNNQRRGILTRVLDWFSPF"
     gene            1694..2797
                     /locus_tag="PKW60_08020"
     CDS             1694..2797
                     /locus_tag="PKW60_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HPJ99417.1"
                     /translation="MCRRMFLLLCALALFGGMLLPAAAHASRIKDLCTVQGAQENALS
                     GVGLVVGLAGTGDGNSDALRRQQRLLERLEIDVDQVGGLNSDNIAVVVVDALYPPFAK
                     QGTRIDIRVNALYDCKSLEGGTLLQTMLYGIDGEVYAVAQGPLSIGGFGAAKGGAGVR
                     QNHLTVARIPMGATIEREIPSTITDGERVTLQLKRPDFITANTIQEAINSHLKVEAAS
                     ALGPGAINVRIPEDRQHDLVAFMASIQNITVDPDFPARVVINERTGTLVIGGEVIIRP
                     CQVAHGNLTLEVATTPVVSQPAPFSEGETVVTGVTDMRVTQDAGYLMPVQGTSARDVA
                     MALNALKVTPRDMISIFQALREAGALDADVEIM"
     gene            2797..3165
                     /locus_tag="PKW60_08025"
     CDS             2797..3165
                     /locus_tag="PKW60_08025"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HPJ99418.1"
                     /translation="MLYVNPLEAPHAFEHGPLTTDPARRRMALKEMEQYFARLLLREM
                     RATVPQGGLFPRSAQQAHFEEMLDDALSAEMAESGQLGLARMLEEQLRIGEMQKKLQR
                     AAPHGSHSAFSAAGSDLAVK"
     gene            3210..3719
                     /gene="flgN"
                     /locus_tag="PKW60_08030"
     CDS             3210..3719
                     /gene="flgN"
                     /locus_tag="PKW60_08030"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HPJ99419.1"
                     /translation="MNTLLDRLCDALDDELLRQETLLAICRKKREAIRALDVEPLEAH
                     TAALEVVLRETFEAEDTRHRLLREAVEQMGLPPEKHTLSQLIGVVPEPWKSRLNYFQC
                     QLRKTLETTQAVTRAYSRELRHYLRLTQTQFARLGLESKEQKAGLYGPRAWKPAMSGV
                     SSALVDQRG"
     gene            3726..4640
                     /gene="flgL"
                     /locus_tag="PKW60_08035"
     CDS             3726..4640
                     /gene="flgL"
                     /locus_tag="PKW60_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HPJ99420.1"
                     /translation="MGAMRVTTQLLVDRSIRNINNQSNRLLTLQEQLATGLRVNRPSD
                     DPIDARRAVSIRALLGKNEQYLANISMVSSPVAETNTSIETILSSFNRAWELALRGAN
                     GTNAQNQLDALALEINQLLEGIFSTANHETNGRYLFGGTRTTTPPYEATRNAEGEITA
                     VHFAGNAEAIHVAISDVLDLQYNEIGLDVFSKNQDIFQTLLAVRDNLRAGDTSALGAV
                     RLDEMETVRDQLTSALARVGSIQNRLDRSSEELEQFAFQLQTQLSDAIDADYADVITR
                     LQAQTNAFQAALSAAARAIMPSLLDYVR"
     gene            complement(4659..4997)
                     /locus_tag="PKW60_08040"
     CDS             complement(4659..4997)
                     /locus_tag="PKW60_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HPJ99421.1"
                     /translation="MKLKEDAELLRIFIGESDKYCGKPLYEAIVKLAREKGLTSATVM
                     RGIMGFGADSRLHTAKVLRLSEDLPIVVELIGHPERIEAFLPELDAMIQEGLMTLEKV
                     QVIAYQSNTE"
     gene            complement(5011..5388)
                     /locus_tag="PKW60_08045"
     CDS             complement(5011..5388)
                     /locus_tag="PKW60_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HPJ99422.1"
                     /translation="MGGKLLFIGFAGAGGALARYALAGLVQKVSGGVFPWGTFVVNIA
                     GCFAFGLIWAITEKRLAFAAESRVYLLTGFLGAFTTFSTFAFETGAMMRDGEYLLATA
                     NLGIHNILGICALFIGLALGTLI"
BASE COUNT         1173 a         1803 c         1610 g         1020 t
ORIGIN      
        1 aaatgctgca aacggccaac aatgtcagac ggtaagcagg gaggttgatg ccctatgaac
       61 cgtgaacaga cacctcaccg cctgttgttc gcatgggccg cagcggctgt gcttttcctg
      121 gccgccgtcc gcgcggacgc gctcaatgtg gtcctgctcc gtgacgaggc cttcgtgaag
      181 gggccgcagg ttgccctggg cgatgtggcg gacatcgaag gcgcggaggc agaagccctg
      241 gccgggcttg agatcctgcc tgccgccgcg ccgggctgtg tgaaacggat ccacgcgtcg
      301 atgctgcgct cccggatcat gacggccggg taccgcccgg aagatttcga gatcaccggc
      361 gccgagagcg tcaatgccac gacgctctgc gtagagctta cacgcgagat gctggtcgat
      421 gacctgcgcc ggttcattga actcgagatg ccgtgggatc ctgccaacgc cacggtcgat
      481 atcgatcccc ccacgagcaa gctcgtcctg cccgaaggcg atatcgtgct cgaatggcgg
      541 cccgcagccc tgtttcagta tctcggcaag ggcaccatgc ggggggaagc cctcgtggac
      601 gggcaggtcc gcgatgtcat ctattgccag gtcaatgtgc aggcctacgg cgatgtggtc
      661 gtggcctcga gcgatatccc ccgcggcgcg atcatctccc gcggcgattt gagcgttgcc
      721 aaacgcgccc tgtcggctat gcgcaacggc tattacagtg atcccgaaga gctggccggc
      781 atggtcgctc gaagcaccat ttttccggac acggttctca cctcgcgcca cgtaatgccc
      841 cggcgcatta tcaaacgcaa ccaggtcgtc acggtggagg tgcgcgcggg cacgctgatg
      901 gtacgcgacc gcgcccaggc gatgagcaat gccgctgcgg gcgacatcat catctgccag
      961 cgcatgaact ccaaggaaga attccagggc attgtccgcg aagacggcat tgtcgaagtg
     1021 tactaggggc agggaggttc atcgtgaaaa aaacgtatct ggtggcggtg ctgctcgcgg
     1081 cggtgctggc cgtccccgtc agggccgatt cccttttctc gaaaggagta gccaagcgcg
     1141 gaccggcggt gtccaataag cacacgcgtt tcacggaagg cgacatcatc atcgtgctcg
     1201 tccgcgaaaa catcgatgcc actacccggg ccaacaccaa cacaaagaag gaatccgagc
     1261 ttatcgcgga ggcgcccatc aacagcaaca ccttcctggt ggcgccgaaa cctgatgggc
     1321 tcggtatcgt gaagccggag cgcctgcccg tgtgggacat caatgttgaa aacgagcaca
     1381 aaggccgggg cgacacgtcc cgcaccaata aattgaccac taccatctcg tgcatcgtga
     1441 cgaaagtcca tgacaacggc aatctcgaaa tcgaaggcaa caagcgcgtc acggtgaacc
     1501 gcgaggattc aacgatactc gtcacgggta ccgtccgggc ccgcgacgtc tcgcctgaaa
     1561 acacggtgcc gtcggaccgg gtggccaatg caatcgtgga gcttaagggg aagggcccgc
     1621 tctggaataa ccagcggcgc ggcatcctga ccagagtgct cgactggttc tccccattct
     1681 agaggatgtg gcaatgtgtc gacgaatgtt cctgttgctg tgtgcattgg ctctcttcgg
     1741 aggaatgctg ctccctgccg ccgcccacgc cagccggatt aaagacctgt gcaccgtgca
     1801 gggagcccag gaaaacgccc tgagcggcgt cggcctggtc gttgggctcg cggggacggg
     1861 tgatggaaac agcgacgccc tgcgccgcca gcagcgtctg ctcgaacgcc ttgaaatcga
     1921 cgtggatcag gtcggtggtc tcaattccga caacatcgcc gtcgtggtgg tggatgcgct
     1981 ctatccgccg tttgccaagc aaggcacgcg gatcgacatc cgcgtgaacg ccctctacga
     2041 ctgcaaaagc ctggaaggcg gcacgctcct ccagaccatg ctctacggga tcgatgggga
     2101 agtctatgcc gtggcccagg gacccctttc catcggcggt ttcggcgcgg ccaaaggcgg
     2161 cgccggcgtc agacaaaacc atttgaccgt ggcgcgcatc cccatgggcg cgaccatcga
     2221 gcgcgaaatc ccgtccacca tcaccgacgg cgaacgcgtt accctccagt tgaagcgccc
     2281 cgacttcatc acggccaaca cgattcagga ggcgattaac agtcacctta aagtcgaggc
     2341 tgcctcggct ctcgggccgg gcgccattaa tgtgcgcatt ccggaagatc gccagcatga
     2401 tctggtggcc tttatggcga gcattcagaa catcacggtg gatcccgatt ttcccgcgcg
     2461 cgtggtgatc aatgagcgta ctggaacgct ggtcattggc ggtgaggtga tcatccgccc
     2521 ctgccaggta gcccacggca acctgaccct ggaagtcgcc accaccccgg tcgtcagcca
     2581 gcccgcacca ttttccgaag gagagacggt tgtcacgggc gtaaccgata tgcgcgtcac
     2641 ccaggacgcc ggctacctga tgccggtcca gggtacctcg gccagagacg tcgccatggc
     2701 attgaacgcc ctcaaggtga ctccgcgcga catgatttcg atcttccagg cattgcgcga
     2761 agccggcgcg ctcgatgcgg atgtggagat catgtaatgc tctatgtaaa cccgctcgaa
     2821 gcgcctcatg catttgaaca cggccctttg acgacggacc ctgcccgccg ccgcatggct
     2881 ctgaaggaaa tggaacagta tttcgcccgc ctcctcctgc gcgaaatgcg cgccactgtc
     2941 ccccagggcg ggctcttccc gcgcagcgcc cagcaggccc attttgaaga gatgctcgac
     3001 gatgccctca gcgctgaaat ggccgagagc ggccagctgg gcctcgcccg catgctcgaa
     3061 gagcagctcc gcatcggcga aatgcaaaag aaactccagc gggcggcgcc gcacggttcc
     3121 cattccgcgt tctctgccgc cggctccgat ttagcggtca agtaaacccc ggaccctgcc
     3181 gataatcagg gcatcatgag gtggttacgg tgaatacgct gcttgacaga ctctgcgacg
     3241 ccctcgacga cgaactcctg cgccaggaaa ccctgctggc catctgccgc aagaaacgcg
     3301 aggcgatccg cgcgctggat gtcgagccgc tcgaagcgca caccgccgcc ctcgaagtag
     3361 tcctgcgtga gacgtttgaa gcggaggaca cgcggcaccg cctgctgcgc gaggccgtcg
     3421 aacagatggg gctgccgcct gaaaaacata ccctttccca gcttatcggg gtggtcccag
     3481 agccttggaa gagccgtttg aactattttc aatgccagct gcggaagacc ctcgagacca
     3541 cgcaggccgt cacccgcgcc tattcccgcg aactgcgcca ttatctcagg ctcacccaga
     3601 cccagttcgc ccggcttggg ctggaatcca aagaacagaa agccgggctg tacgggccgc
     3661 gtgcgtggaa acctgcaatg tccggcgtct cttcggcgct cgtcgaccag agaggttaat
     3721 cagacatggg cgcaatgcgt gtcaccaccc agctgctcgt tgatcgctcg atacgcaaca
     3781 tcaacaacca gtcaaaccgg ctcctcaccc tgcaggaaca actggcgacc gggttgcgcg
     3841 tcaatcgccc gtcggatgat cctatcgacg cacggcgcgc tgtcagcatc cgggctttgc
     3901 tcggcaaaaa cgaacaatat ctcgccaata tctccatggt gagttccccg gtggcggaga
     3961 ccaacacttc cattgagacc atcctgagtt cgttcaatcg ggcctgggaa ctggctctgc
     4021 gcggcgccaa cggaaccaac gcacaaaatc agcttgatgc gctggccctc gaaatcaatc
     4081 agctcctcga aggcatcttc agcaccgcta accacgagac caacgggcgg tatctgttcg
     4141 gcggcacccg cacaacaacg ccaccctacg aggcgacgcg caacgccgaa ggcgagatca
     4201 ccgcagtgca ttttgcaggc aacgccgaag cgattcacgt cgccatttcc gatgtcctcg
     4261 acctccagta caacgagatc ggcctcgacg tcttctccaa aaaccaggac attttccaaa
     4321 ccctcctggc tgtgcgcgac aacctgcgcg cgggcgacac cagtgcgctc ggcgccgtgc
     4381 gcctggacga aatggaaacc gtgcgcgacc aattgacctc tgcgctcgcc cgcgtcggca
     4441 gcattcagaa ccgcctggat cgaagcagcg aggagctcga gcagttcgcg ttccagttgc
     4501 agacgcagct gtccgacgcc attgacgccg actatgctga cgtgatcacg cggctccagg
     4561 cccagaccaa tgcgtttcag gcggccctct ccgccgcggc ccgggccatc atgcccagcc
     4621 ttctcgatta cgtccgctag accctgttcg gcgcagatct actccgtatt agactggtat
     4681 gcaataacct gcactttttc cagggtcatc aggccttcct gaatcatcgc gtccagctcg
     4741 ggcaggaatg cctcaatgcg ctccggatgg cctataagtt caaccacaat tggcaggtct
     4801 tccgataagc ggagcacttt tgccgtgtgc aggcggctgt ccgcgccaaa gcccatgatg
     4861 ccccgcatga cggtggcact ggtcagcccc ttttcacggg cgagtttcac gatggcctcg
     4921 tacagaggct tcccacaata cttgtcgctc tctccaatga aaatgcgaag cagctcggcg
     4981 tcttctttca gcttcatgag gcggcccccg ctaaattaac gtgcccagcg ccagaccgat
     5041 aaagagcgca caaatcccca agatgttgtg aatgcccagg ttcgcggtcg cgagaaggta
     5101 ctctccgtcc cgcatcatgg cgcccgtctc aaatgcaaag gtcgaaaacg tagtaaatgc
     5161 tccgagaaac cctgtgagca aatacactcg gctttcggcc gcaaacgcga gccgtttctc
     5221 ggtgatggcc cagatcaagc caaacgcaaa acaccccgcg atgttcacaa caaacgtgcc
     5281 ccacggaaat acgcctcccg acaccttctg gaccagcccc gccagggcat atcgagcgag
     5341 cgcaccccct gcgccggcaa aaccgatgaa cagcaattta cctcccataa ttcctccatt
     5401 ccggcaatct ctcaatgaaa tctcgacgtt ttgggagcgc tgatatggta ctctccggct
     5461 gctggtaact gaaatacctg tcgggcaggc gccgacagaa ttccggcatc cggctcgcgg
     5521 ccgggcgagt caggacgcac agcgagttcg tccctgtcaa gccatttgct aatcttaccg
     5581 gggcgtatat cgtcgcgcca tttggt
//
LOCUS       DAOPBQ010000213         5575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12522_length_5575_cov_3.831884, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000213 DAOPBQ010000000
VERSION     DAOPBQ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12522_length_5575_cov_3.831884"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..835
                     /gene="grpE"
                     /locus_tag="PKW60_08050"
     CDS             161..835
                     /gene="grpE"
                     /locus_tag="PKW60_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPJ99423.1"
                     /translation="MKKKNTKKSFLEEQLEAEARKQAGASDPGDPAADTPGAEAHSEG
                     LPGDGTRSTGGAAAAEPVDVEALLEERDQLKDQLLRARAEFDNFRKRNARDYERLRKT
                     AAEALVRDLLPVMDHLELALKHADDASNAVVEGVQMVARQFADVLGRHGVVPIPALGE
                     AFDPNVHEAMMQRADDDAEPNTVLEEFQRGYCMGDLVLRPSKVVVSSAPSAASADSSD
                     GGSEEG"
     gene            925..2880
                     /gene="dnaK"
                     /locus_tag="PKW60_08055"
     CDS             925..2880
                     /gene="dnaK"
                     /locus_tag="PKW60_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPJ99424.1"
                     /translation="MGKVIGIDLGTTNSCVAVMEGGEPAVIANAEGNRTTPSVVAFTK
                     NGERLVGAVAKRQAITNPQNTVFSIKRFMGRRHNEVASEEKIVPYKVGENPTGDCQVE
                     LMGQTYRPPELSAMILQKMKETAESYLGTTVTQAVVTVPAYFNDSQRQATKDAGRIAG
                     LEVLRIINEPTAAALAYGLDRKKNEKVAVYDLGGGTFDVSILAIGDDSFEVLSTNGDT
                     HLGGDDFDQRIIDWLAEEFLRDQGFDLRKDPMALQRLKEAAEKAKCELSTTMQTDINL
                     PFITADASGPKHLNYTLTRAKLEQLCDDLLQRSKSPCFRALEDAGLSAQDINEVILVG
                     GMTRMPAVAEIVKAIFAREPHRGVNPDEVVAIGAAIQGGVLSGEVKDVLLLDVTPLSL
                     GIETLGGVCTKLIERNTTVPVTKRQIFSTASDSQTTVTIHVLQGEREMADDNRTLGKF
                     DLFGIPPAPRGIPQIEVTFDIDANGIVHVSAKDLATGKEQKIRIESSSGLSESEIDKM
                     VKDADAHRQEDEEKKHRIQVRNNADSMVYQCEKLLRENADKVSAADKKAVEDAVQEVK
                     KALESGDTPSIEAAMERLTQAQYKLSEALYKSSAAQDPAGEHASTGAGQGDGSETPTG
                     GTSEDGPMDADFTVVDEEENENGTKQN"
     gene            2997..4148
                     /gene="dnaJ"
                     /locus_tag="PKW60_08060"
     CDS             2997..4148
                     /gene="dnaJ"
                     /locus_tag="PKW60_08060"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPJ99425.1"
                     /translation="MATKNLYDILGVSREASQEDIRKAYLKLAHKYHPDKTGGDKAAE
                     EKLKEINAAYDTLKNPEKRSQYDRFGQAGPGFGPGFGGNAGFGGFGFGFDNAGGFETP
                     FEDFFDVLFGRQGAGRGRRRPAARPGNDLERRVTISLREAAAGVKRAIRFSRMEKCAD
                     CKGSGAAPGTQPRTCAECQGTGQVRRAQGFFSITQTCRQCGGRGWAIETPCSKCRGSG
                     RVQTQRELAIDIPAGVDTGSRLRIPGEGEPGENDGPRGDLYIVIEVETHDVFVRDGND
                     LICEAPVSFPQAALGATVRVPSLQGEAEVKIPAGTQSGQLFRLRGLGIPDIRGYRTGD
                     QIVRVVVETPTRLNREQKELLQRFQELTREQNYPAHVRFLGKLKEWLGG"
     gene            complement(4168..>5575)
                     /locus_tag="PKW60_08065"
     CDS             complement(4168..>5575)
                     /locus_tag="PKW60_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPJ99426.1"
                     /translation="PHLMARNREAVLTSGAIPTTLFRLAGPMSIGLFAQISFTLVDTL
                     FVARLGTDALAALGFAFPVVFVISNITNGLGTATSAVVSQAIGRGDQHLVRRYTTDSL
                     ILSFLIVVVLSIGGYLTIDPLFTALGARGETLHLVRQYMAITYLGTAFIVIPTISINA
                     IRATGDTVTPSTIMVVAALVNVVLDPIMIYGWFGFPRMEMVGAALATVIGRIMSCIAA
                     LVFLHFRERMISFRRPRMNEVLRSWRSILAIGVPASATNIMVPLSNLFVTAIAARAGK
                     EVVAALGAGMRVTAFTSIPVYALSASLVPFVGQNWGARRYRRARHSKRHSIQFGTVWG
                     GLCFVGLWVTAPLIAGIFSQDAQVRREIVLYLRIVPLGHALQNSFIFMTSILNAIQRA
                     MVSTILMAVRMFVLYVPLSVLGAWWWGGAGLFGGMAAANTLSGIMATVVSGYLVRKPR
                     RHAGQAALLAAQTGIPLE"
BASE COUNT         1218 a         1701 c         1767 g          889 t
ORIGIN      
        1 tgccgtattc gcggctctcg ggcctggttg aatacaccgc agccctcctg ggaagctacc
       61 tgaccggtat cgcggggatg tcgacggatg acgatgtcgg gacgaacccg ctcgtgggtg
      121 gtcccagcga cggaaatcac gactagagac tacgcagggc atgaagaaga aaaacaccaa
      181 aaagtcgttt ctggaagagc agcttgaagc ggaagcccgc aagcaggccg gcgcgagcga
      241 cccgggagat cccgccgcgg acaccccggg cgctgaagcg catagcgaag gcctccccgg
      301 ggatgggacc cgctcgaccg gcggagctgc agctgcggag ccggtggatg tcgaggcctt
      361 gcttgaagag cgcgaccagc tcaaagatca gctcctgcgc gctcgcgccg agttcgacaa
      421 tttccgcaaa cggaatgcac gcgactacga gcgcctccgc aaaacggccg cggaagcgct
      481 ggtgcgcgat ttgctgcccg tcatggacca tctggaattg gcactgaaac atgccgatga
      541 cgcgtcgaac gcggtcgtgg aaggcgtcca gatggtggcc cggcaatttg cggacgtgct
      601 gggccgccac ggcgtggtgc ccattccggc tttgggagag gcctttgatc ccaacgttca
      661 cgaggccatg atgcagcggg ccgacgacga cgccgagccc aatacggtgc ttgaggaatt
      721 tcagcgcgga tactgcatgg gggatctggt gctgcgcccg tcaaaggtcg tggtatcttc
      781 agcgccctct gcggcatctg cggactcatc tgacggggga tcggaagaag gataggcgtg
      841 cccggcgatt cgagctcgcg gccggggccg tggtgcgcgc agaaggcact ggtaattcag
      901 ccaatttacg gaggtttata gacgatgggc aaggttatcg gcattgatct tggaacgacc
      961 aactcatgcg tggcggtcat ggagggcgga gagcctgcag ttattgcgaa tgcggaaggc
     1021 aaccggacga caccgtccgt ggtcgctttt acgaaaaacg gcgaacggct cgtgggcgct
     1081 gtcgccaagc ggcaggccat tacgaatccg cagaataccg tattttcaat caagcgtttt
     1141 atggggcgcc gccacaatga agtcgcgtca gaagagaaga ttgtgccgta caaggtcggg
     1201 gaaaatccca ccggcgactg ccaggtcgaa ctcatgggtc agacctatcg ccctcccgag
     1261 ctgtccgcga tgattttgca gaaaatgaaa gagaccgccg aaagctatct cggcactacg
     1321 gtgacccagg ccgtggtcac tgtgcccgcg tattttaatg attcgcagcg ccaggccacc
     1381 aaggacgccg ggcgaatcgc gggcctggag gtcctgcgca tcatcaacga accaactgcc
     1441 gcggcactgg cttatgggct cgaccgcaag aaaaacgaaa aagtggccgt gtacgacctc
     1501 ggcggcggaa ccttcgacgt ctcgatcctc gcgattggcg atgacagttt cgaagtgctc
     1561 agcaccaacg gcgacacgca tctcggtggc gacgattttg atcagcggat aatcgactgg
     1621 ctggcggagg agttcctccg cgaccagggc tttgacctgc gcaaagatcc catggccctg
     1681 caacggctca aggaagccgc tgaaaaagcc aaatgtgaac tctccacgac gatgcaaacg
     1741 gatatcaatc tgccgttcat caccgccgac gcctccgggc ccaaacacct caactacacc
     1801 ctgacccgcg cgaagctcga acaactgtgc gatgacctcc tgcaacggtc aaaaagcccc
     1861 tgcttccgcg cgcttgaaga tgccggcctg tcggcccaag acatcaacga ggtcatcctg
     1921 gttggcggca tgacgcgcat gcccgccgtg gccgagatcg tcaaggccat ctttgcccgg
     1981 gaaccgcatc gcggcgtgaa tccggacgaa gtggtcgcga tcggcgccgc gatccagggc
     2041 ggcgtgctct ccggcgaagt caaagacgtc ctgctgctcg atgtgacacc gctttctctc
     2101 ggcatcgaga ccctgggcgg cgtgtgcacc aagctcatcg aacgcaatac cacggttccg
     2161 gtgaccaaac gccagatctt ctcgaccgct tccgacagcc agaccaccgt gacgattcat
     2221 gtgctccagg gggagcgtga aatggccgac gacaaccgta cgctcggcaa gtttgacctc
     2281 ttcggcattc cgccggcgcc ccgcggcatc ccccagatcg aagtcacgtt tgacatcgac
     2341 gccaacggga tcgtgcatgt ctccgccaaa gacctcgcta ccggaaaaga acagaagatc
     2401 cgcattgaat cttccagcgg cctgtctgag agcgagatcg ataaaatggt taaggatgcc
     2461 gacgcacacc gccaggaaga tgaggagaaa aagcaccgga tccaggtgcg caacaatgcc
     2521 gattccatgg tgtatcagtg tgaaaaactc ctcagggaaa atgcggacaa agtcagcgcc
     2581 gccgacaaaa aagcggtcga ggatgccgtg caggaagtga agaaggctct ggaaagcggc
     2641 gataccccct cgattgaagc ggcgatggag cgcttgaccc aggcgcagta caaactttct
     2701 gaagcgctgt acaaaagctc ggcagcgcag gacccggccg gcgagcacgc ctcgacgggc
     2761 gcgggacagg gcgacggcag cgaaacgcca acgggcggca cgtctgagga cggccccatg
     2821 gatgcggact tcacggtcgt cgacgaagaa gaaaacgaaa acggaaccaa acagaattga
     2881 ggtaaagcga atccgcgcga accggcccgg cggcatgatg ccgggtggac cgcgggtgcg
     2941 tgtgcagaga cgcgcgccgg ggggccggtg cgggcggatt gtgtggatac ccatccatgg
     3001 ctacgaagaa tctgtatgac atactggggg tttcccggga ggcctcgcaa gaggacattc
     3061 gcaaggccta cctgaagctt gcccataaat accatccaga caaaaccggc ggcgacaagg
     3121 cggctgaaga aaagctcaaa gagatcaacg ccgcgtacga tacgttgaaa aatcccgaaa
     3181 aacgctcgca atatgaccgg ttcggccagg ccggccccgg tttcggcccc ggcttcggcg
     3241 gcaatgcggg tttcggcgga ttcggctttg gctttgacaa cgccggcgga tttgagacgc
     3301 cgttcgaaga cttcttcgat gtcctcttcg gccggcaggg cgccgggcga gggcgccgcc
     3361 gtcctgcggc gcgcccgggc aacgatctcg agcggcgcgt cacgatatcg ctgcgggagg
     3421 cggcggcggg cgtgaagcgc gcgatccggt tctcccgcat ggagaaatgc gcggactgca
     3481 aaggctccgg ggcggctccc ggcacgcaac cgcgaacctg tgccgaatgc cagggcaccg
     3541 gccaggtacg ccgggctcag gggtttttca gcattacgca gacgtgccgc cagtgcggcg
     3601 gccgcggctg ggcgatcgaa acgccctgca gcaaatgccg cggttccggc agagttcaga
     3661 cacagcgcga actggccatc gacatcccgg ccggtgtgga caccggttcg cgcctgcgga
     3721 tcccggggga gggcgagccg ggcgaaaacg acggcccgcg cggcgacctc tacatcgtaa
     3781 ttgaagtgga gacccatgat gtcttcgtcc gcgatggaaa cgacctgatc tgcgaggcac
     3841 cggtcagttt tccccaggcc gcgctcggcg ctacggttcg ggtgccgtcg cttcagggag
     3901 aggcggaggt caagatcccc gcggggaccc agtcagggca gcttttccgc ctgcgcggtc
     3961 tcggcattcc cgatatccgc ggctatcgca ccggtgacca gattgtgcgg gtggtcgtcg
     4021 agacccccac gcgcctcaac cgcgaacaga aagagctgct ccagcgattt caggaactga
     4081 cgcgcgagca gaattacccc gcacacgtcc gcttcctcgg caaactcaag gaatggctcg
     4141 gaggatgacc tggcccgaag cgcccgatca ttccagtggt atgcccgtct gcgcggcgag
     4201 aagggcggcc tgcccggcat ggcggcgcgg tttgcgcacc agataacctg agactacggt
     4261 tgccatgatg cccgaaaggg tattggcggc cgccatccct ccgaacagcc ccgcgccgcc
     4321 ccaccaccag gcacccaaga cagacagcgg cacatacagc acgaacatcc gtacggccat
     4381 caggatcgtc gacaccatgg cgcgttgaat cgcgttcagg atgctggtca tgaagatgaa
     4441 cgagttctgc agcgcgtgac ccaggggaac aatgcgcaga tagagtacaa tttcgcgccg
     4501 gacctgggcg tcctggctga aaatgcccgc gatgagcggt gcagtgaccc acaagccgac
     4561 aaagcacagc cctccccaga ccgtgccgaa ttgaatgctg tgccgcttac tgtggcgcgc
     4621 gcgccggtag cggcgggcgc cccagttctg cccaacgaac ggcaccaggg atgcgctcag
     4681 cgcatacacc gggatggagg tgaacgccgt cacgcgcata ccggcgccga gcgccgccac
     4741 aacttccttt ccggcgcgcg cggcgatcgc ggtcacgaac aggttggaga gcggcaccat
     4801 gatattggtc gcgctggccg gcacgccgat cgccaggatg ctgcgccacg accgcaagac
     4861 ctcgttcata cgcgggcgcc gaaatgagat catgcgctcg cggaaatgaa gaaacaccag
     4921 cgcggcgatg cacgacatga tccggccgat aacggtggcg agggccgcgc cgaccatttc
     4981 catccgggga aacccgaacc agccatagat catgatcgga tccagcacca cgttcaccag
     5041 tgccgccact accataatcg tgctcggcgt gaccgtgtcg ccggtcgcgc gaattgcgtt
     5101 gatgctgatg gtcggaatca cgatgaacgc ggtgcccagg tacgtaatcg ccatgtactg
     5161 ccgcaccaga tgcagggtct ctccccgggc gcccagtgcc gtgaacaacg gatcaatagt
     5221 cagatagccg ccgatggaga gcaccacaac gatcaggaac gagagaatca ggctgtccgt
     5281 cgtatagcgc cgcacgagat gctggtctcc acgcccgatc gcctgcgaaa cgaccgcgct
     5341 ggtcgcggtg cccagcccat tggtgatgtt gctgatgaca aagaccaccg ggaacgcgaa
     5401 ccccagcgca gccagcgcat ctgtccccag cctggcgaca aacagcgtat cgaccagtgt
     5461 gaatgagatc tgcgcaaaca gaccgatgga catggggcca gccagccgga agagcgtcgt
     5521 ggggatggct ccggaagtaa gcaccgcttc tcggtttctg gccatgaggt gtggt
//
LOCUS       DAOPBQ010000214         5546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12582_length_5546_cov_4.075396, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000214 DAOPBQ010000000
VERSION     DAOPBQ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12582_length_5546_cov_4.075396"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1544
                     /locus_tag="PKW60_08070"
     CDS             105..1544
                     /locus_tag="PKW60_08070"
                     /inference="COORDINATES: protein motif:HMM:NF018606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99427.1"
                     /translation="MRRGKWSGVIVAVILAAGLSGGAPVSAGEAGETGYLERVVAYAN
                     AMLEHGQDTYGETSTPLFVEELDRTTLQMLADGALEAAAAIPYEDWGIRSHDRMLGGA
                     NPQHCTNLYQILYALTAITGEARYAEAADASLAYFLEHCQSGATSLFYWGEHAGWDLR
                     LDAPLETSSGNIHEFYRPWPLWERSWELAPEPCLRFARGLWEHQIGDQETGAYSRHAK
                     ISQHGPATTAPYARHGGFYIETWAYAYKKTGDAVFLQAIETVLNGLERARLNDGGYLV
                     AGSLEKGSRRPFNISLAVSLELAAPDVPPEPAATMRAVAAANDEVFAQNYTDPDAGQV
                     QSENLWTNAYGSGPRTGHANLFMLRYRQTGKDLYRRALLVEADLYREEQINLSSPVWP
                     GTVGEAIWLMLDAHELTGNTGYLQTATRFAREGIALFLQDGPLPKASHVHDHYEAVTN
                     GDTLMMALLRLWKVQNPTSAPSTLTYIDR"
     gene            complement(1599..2348)
                     /locus_tag="PKW60_08075"
     CDS             complement(1599..2348)
                     /locus_tag="PKW60_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99428.1"
                     /translation="MTNQRHSWVEVDPVELQEALRQLARLPKPLKRFRRSILWFAEGH
                     VVLSDGLNEVRVEGTGLWKVPALMKFPAFKVLRELAQRANPEQPLLIEGVDSEVRVGN
                     SCFTCNFQKVELNEQGVLQSERERQRVTEDNADIVGTGPVQEYDAAKTLHQIRELYPD
                     SSWYGVIEFNLVTIPAFPYLVIYVRPACVEFRIVTDLAGSPTDWRTPSEIWKRLDHAI
                     VRELGLEALIQWAGEAAAEKAEELRSRNEGR"
     gene            complement(2817..4514)
                     /locus_tag="PKW60_08080"
     CDS             complement(2817..4514)
                     /locus_tag="PKW60_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPJ99429.1"
                     /translation="MSDYYSRYAEILAERYEKVPTDRVHQTWLHLLPRTKANVLDVGA
                     GSGRDAGFFARLGHEIVAVEPCSELRDIAQRLHPEAAITWLNDRLPSLKAVYALNLRF
                     DLILLSAVWMHVPATSRERAFRKLLGLLKPGGILVLTLRQGPDPDERQMYPVSSEELR
                     HFAAQFAAEVVIQAKQRDEMGRPGIDWEVVVCRIPDDGTGALPLLRHIIINDPKSSTY
                     KLALLRILLRIADGAQGVVLERTEDFVTLPFGLVALYWVKIFKSLVLDNEFFQQPAGS
                     GNLGFDRAAFRALKDISSNDLRIGSRFDGEIARNLAMALRDARNTIKKMPAFYTKYPG
                     SSEPVFPCTVAAAKAADTMSLDKESLSAFGTFQVPTHLWDAMTSYACWIEPAIVSEWA
                     KLMVGYDQRAGKERPLDHYYEGLKWLDEQRNTKEIREIVERLRNKGKAVFCTWTGKKL
                     RDRYDIDHCFPFAYWPNNDLWNLLPTDARVNSAKSNRLPTAEMLERAKDRILEWWDIG
                     YAAPGYSERFMTEANASLPVIDRFDLGNDLECVFSRVRHQRVRLKSFQQLADWEVAG"
     gene            complement(5015..5090)
                     /locus_tag="PKW60_08085"
     tRNA            complement(5015..5090)
                     /locus_tag="PKW60_08085"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5055..5057),aa:Thr,seq:cgt)
     gene            complement(5099..5174)
                     /locus_tag="PKW60_08090"
     tRNA            complement(5099..5174)
                     /locus_tag="PKW60_08090"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5139..5141),aa:Phe,seq:gaa)
BASE COUNT         1158 a         1671 c         1590 g         1127 t
ORIGIN      
        1 cctgtttttc atgcctgccc gccaggccag gccggtccaa aagctgaaaa ttcgctgcga
       61 ttcaggcaca ctggggcata tcagtcaatg gaaagggaga aagcatgcgt cgtggcaagt
      121 ggagcggagt catcgttgcg gtcatcctgg cggctggttt gagtggcgga gcgcctgttt
      181 ccgccgggga ggccggcgag accggctacc tcgagcgggt ggtggcctat gccaatgcca
      241 tgcttgaaca cggccaggac acgtacggcg agacctccac accgctgttt gtggaggaac
      301 tcgaccgcac cactctccag atgctcgcgg acggagcgct ggaagcggcc gcagccatcc
      361 cgtacgagga ttgggggata cgctcccacg accgcatgct gggcggcgca aatccgcagc
      421 attgcaccaa tttgtaccag attctgtacg cgttgaccgc tatcacggga gaggcccgct
      481 atgcggaggc cgccgatgcc tcgctggcgt actttttaga acattgccag agcggagcca
      541 ccagcctttt ctattggggt gaacacgcgg gctgggatct ccgcctcgat gcgccgctgg
      601 agacctcgtc cggaaatatc catgaatttt accggccctg gcccctgtgg gaacgcagct
      661 gggagctcgc gccggaaccg tgcctccgat ttgcccgcgg gctgtgggaa catcagatcg
      721 gcgaccagga aaccggcgcc tattcgcgcc acgccaaaat cagccagcac ggccccgcta
      781 cgaccgcgcc ctacgcgcgg cacggcggct tctacatcga aacctgggca tacgcctata
      841 aaaagacggg cgatgcggtc ttccttcagg caattgaaac cgtcctgaac ggcctggaac
      901 gcgcgcgcct caacgacggc ggctacctgg tcgccggtag cctcgaaaaa ggcagccgca
      961 gacccttcaa catctcactg gccgtgtcgc tcgaactcgc agcgcccgac gtaccgcccg
     1021 aacccgccgc gacgatgcgc gctgtcgccg ccgccaacga cgaggttttc gcgcagaact
     1081 ataccgaccc tgacgccggg caggtacaga gcgaaaacct ctggaccaac gcctacggga
     1141 gcggcccccg caccgggcac gccaacctgt tcatgctgcg ctaccggcag accgggaagg
     1201 atctctaccg ccgcgcgctc ctcgtcgagg ccgacctcta ccgcgaagaa cagatcaacc
     1261 tgagcagccc ggtctggccc gggaccgtcg gcgaagcgat atggctcatg ctcgatgccc
     1321 acgaattgac gggcaatacc ggctatctgc agaccgccac ccgctttgcc cgggaaggca
     1381 tcgccctgtt tctccaggac ggccccctgc ccaaagcctc gcacgtccac gaccactacg
     1441 aagccgtcac caacggcgat accctcatga tggccctgct ccgcttgtgg aaggtccaga
     1501 acccgacatc ggcgcccagc accctgacct acatcgaccg ctgaaacccg tatacaacgc
     1561 acacagcagg gatgcccagc gggtcagtgc cagcctggtc agcggccttc gttgcgggag
     1621 cgaagttcct ctgccttttc ggcggcggct tctccggccc actgaatgag ggcttcaagg
     1681 cccaattcgc gcacgatggc atggtccagg cgtttccaga tctcggaggg tgtgcgccag
     1741 tccgtcgggc tgccagcgag gtccgtcacg atacggaact cgacacaggc gggccgcaca
     1801 tagattacca gatagggaaa ggcggggatg gtcaccagat taaactcgat taccccatac
     1861 cacgaagaat cgggataaag ctccctgatc tggtgcagcg tcttcgcggc atcgtactcc
     1921 tgcaccgggc cggtgcccac gatgtcggcg ttgtcctccg tgacccgttg ccgctcgcgc
     1981 tcagactgca ggacgccctg ctcattgagc tctaccttct gaaaattgca tgtaaagcaa
     2041 gaattgccca cccggacctc gctgtcgacg ccctctatga gcagcggctg ctccgggttc
     2101 gcccgctggg ccagttcacg cagcaccttg aaggcgggga atttcatcag agccgggacc
     2161 ttccagagcc ccgtgccctc cacacgaacc tcattgagcc catcactcag caccacgtgc
     2221 ccttccgcaa accagagtat ggacctccga aaacgcttca gcggtttggg aagccttgcc
     2281 aattgccgga gcgcctcctg caattccacc ggatctactt ctacccagga atgtcgctga
     2341 ttggtcatgg ccagcaatcc gattcgaact tcatcccgta ccgtgctcct gtaaggttgt
     2401 cctcccccgg cagcccgtgc ccggggcgcc aatacgaaag catagaccat gccaaaccgg
     2461 aaacgcagca gaagaccgca ctccgcgggg gccatccgag accacgaggc gattagcatc
     2521 gccatccggt cgtcgaaaat tgtgcagcac gctcaaaaat agatcaccat gcccacagcc
     2581 aaattgacgc aaccgcccgg caaacatacc ttgcccgcat tcgctgaacc ccgcacaagg
     2641 tagatcaaac gctcaaccgg gcgggccacc cggggcgcgg acctctgggt tcgtcgtacg
     2701 gccgcggcag cctgcgtaaa tcgtggctga ctgaaaaatg accagataca gtcaaatgaa
     2761 ttgcgcgcat ctttcgcgcg acccgcgatc ttgtgggcag cgaatgcgat cactctctat
     2821 ccagcaactt cccagtctgc gagctgctga aagctcttca ggcgtacacg ctggtgtcta
     2881 acgcgagaaa agacgcattc gaggtcgttg ccaaggtcaa aacggtcaat tacgggtaaa
     2941 gacgcgtttg cctctgtcat gaagcgctca gaatacccag gcgcggcata tccgatatcc
     3001 caccactcca gaattctgtc ttttgctctc tcgagcattt ccgcggtcgg taaacggttg
     3061 cttttggcac tgttgactcg cgcatcggtc ggcagaagat tccacaggtc attatttggc
     3121 cagtaagcga acgggaaaca gtggtcgatg tcgtatcggt cgcgcagctt cttgcctgtc
     3181 cacgtgcaga atactgcttt gcctttgttt ctcagccgtt ctactatttc tctgatttct
     3241 ttcgtgtttc gctgttcatc gagccatttc agcccttcgt agtaatggtc cagtggccgc
     3301 tcttttccgg ctctttgatc ataccccacc atgagtttgg cccattcact gacgatcgct
     3361 ggctcaatcc agcaggcgta ggaagtcatt gcatcccaca ggtgcgtcgg cacctggaac
     3421 gtgccgaagg cactaagaga ttctttgtcc agcgacattg tatctgctgc cttggcagcg
     3481 gcgacggtgc acgggaaaac gggttctgaa ctccccgggt attttgtata gaaggcaggc
     3541 attttcttga tcgtgtttcg cgcatccctg agcgccatcg caagattgcg cgctatttcg
     3601 ccatcaaacc ggctgccaat tcgcagatcg tttgaggaaa tgtctttcaa ggcccgaaaa
     3661 gcagcgcggt cgaaacccag attcccgctt ccagctggtt gttggaaaaa ttcattgtct
     3721 aaaacgaggc ttttaaatat ttttacccag tatagcgcta ccaaaccaaa aggaagcgtg
     3781 acaaaatcct cggttctttc gagaacaacc ccctgggcgc cgtcggcaat ccgaagcaaa
     3841 atcctgagca gagccagctt gtacgtggaa gatttggggt cgtttatgat gatatgccga
     3901 agcaagggca gcgcccctgt cccatcgtca ggaattcgac ataccacgac ttcccaatcg
     3961 atgcccgggc gccccatctc atcacgctgt ttggcctgaa tgacgacttc ggccgcgaac
     4021 tgcgccgcaa agtggcggag ttcttcggaa gagactgggt acatttggcg ttcgtcagga
     4081 tctggcccct gtcgaagtgt aagcacgagt atgccacccg gcttgaggag gcctaacaac
     4141 tttctaaaag ctcgctccct ggatgtggca ggtacgtgca tccatacggc actcaagagg
     4201 atcagatcaa agcgtaaatt gagagcatag acggccttga gagatggaag tctgtcgttt
     4261 aaccatgtta tggctgcctc ggggtgaagc cgctgcgcaa tgtctcgaag ttctgagcat
     4321 ggctctaccg cgacgatttc gtgccccagc cgggcaaaaa agcctgcgtc tcggcccgaa
     4381 ccggcaccga catcgagcac gttggccttc gtacgaggaa gcagatgaag ccacgtctga
     4441 tggacgcggt ctgtcgggac cttttcgtac cgctcggcga gaatttccgc gtatctggag
     4501 taatagtcgc tcattgtggc caatgcacct taacgggtga cactcggtag cctatcacag
     4561 atagtccgca gatctcacca gcccgttgac ctaagtcatt cctgacaaag acatttacat
     4621 ctccatcttg tgatggaatg gcgcctcctg gtgtggtaat tccaactttc ctgagcgctc
     4681 tgaaatttta ctttcgtggg gaagcggaga aggcccagcc gatcagaggt tcgcgagaaa
     4741 tggactgact cgttctggct tcgttctgaa tacgtttttc tgagatcatc cgcccgtcag
     4801 aacgaagttc cggtaccagt gcgccccgcc ccaagttccc cgtgtttcag gcggcttttg
     4861 cggtggcggg cgtgggagta ggagctagcg aaatcggcgc tgaggcggca agagtggttg
     4921 ccgcggctca tgaacagggg ggagtctgga gttagcgtgg ggttggagga aaagcaaagg
     4981 acttacggtg atccgcgtaa gtccttgagg tgcctggagc tggagggggg aatcgaaccc
     5041 ccgacctcat cattacgagt gatgcgctct gccaactgag ctactccagc aaaaaaaatg
     5101 gtgccaaggg gcagaatcga actgccgaca cgcggatttt cagtccgctg ctctaccaac
     5161 tgagctacct cggcacgggc acgataatac aatttggagc gggtgaaagt caacgtgtcc
     5221 ccggtgacat tttgcgcggg gcggttgtgt ttgtcgaaag gcggttcatg cgcggctcac
     5281 gagccgggtg gcggcatcta cccggcgcgt gttttcggtg tgattcaatc gttcacaaac
     5341 ctgcctttcc aggggcgtat gcagtggcgt cctgcgcggt gtgctttaag cgggaggaac
     5401 gcgggcgggt cacggttccg gcggcggggt ggttggccgc tcgggctgtt cgcgaaggcg
     5461 gtcgacgtcg aggcgctgca gcattgcgcg gctgggtttg agcccggttt gaggggtgtc
     5521 ttcgggccag ttcagaaagc gcgccg
//
LOCUS       DAOPBQ010000215         5542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12596_length_5542_cov_3.919446, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000215 DAOPBQ010000000
VERSION     DAOPBQ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12596_length_5542_cov_3.919446"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..122
                     /locus_tag="PKW60_08095"
                     /pseudo
     CDS             1..122
                     /locus_tag="PKW60_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005201008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
     gene            312..1238
                     /gene="rbsK"
                     /locus_tag="PKW60_08100"
     CDS             312..1238
                     /gene="rbsK"
                     /locus_tag="PKW60_08100"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPJ99430.1"
                     /translation="MANILVVGSANIDLVTRVPRVPMPGETLLGRSFDTVPGGKGANQ
                     ATAAARLGAAAWFAGCVGNDAFGEMQRRNLAAEGIDLTYLQVHPSEPTGTAVILVADE
                     GQNSIVVTPAANMGIRPEDLAGMAAVFALVDAVLVQLEIPLDTVESALDMARRHAVLS
                     IVDAGPAQQLPDAIIEKADVLSPNETEAEALTGVSIRSLDDARKAGRELLERGARRVV
                     MKLGAMGALYIDDKEWIHVPAFSITPVDTTAAGDAFTAALALVWQERRPGDALTFANA
                     AGALAATVAGAQPSMPTRAALDAFLKAQGTHS"
     gene            1235..1918
                     /gene="rpe"
                     /locus_tag="PKW60_08105"
     CDS             1235..1918
                     /gene="rpe"
                     /locus_tag="PKW60_08105"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPJ99431.1"
                     /translation="MNKDLLYSASIMCANLLRLEGELAALEAAGCDELHFDVMDGQFV
                     PNITLGFDFIKAVRSYTSLPCSAHLMIERPERYIERFVEAGCHSVTVHVEACTHAHRV
                     LNQIKAAGATSGIAINPATALTKLDYLLDSADRVLVMAVDPGFTGQTILKGTFDRVRI
                     LHENLRYRKLDVRIEVDGNITVRNAAILANLGASILDCGTSSIFDGRPPGEALADFRE
                     KVALERKIV"
     gene            2605..3054
                     /locus_tag="PKW60_08110"
     CDS             2605..3054
                     /locus_tag="PKW60_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99432.1"
                     /translation="MKRLLALVLVGVFMVGMAGCSGGAKDAAPAGSGIGESVAPSSEG
                     ASTELSVEPEESAEETSNDTAAAESAGGTELTGTKWQLGDYEINFKDDGKVLVKGGQI
                     STLMPDGAEGEYKIEDGKFSVSILGQSFNGTWDGSKLVVDGNEAVLQ"
     gene            3472..4437
                     /locus_tag="PKW60_08115"
     CDS             3472..4437
                     /locus_tag="PKW60_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPJ99433.1"
                     /translation="MIKTQNLTMHYGPVRALDRVSFEVKQGEIVGLLGPNGAGKSTTM
                     KILTTYLHPTSGTATVGGMDVLENPLGVRKVIGYLPEVLPLYMDMEVRSYLSFVARSR
                     GLGGAKLRERTAAVLEECGLKPMYRKVIRELSKGYRQRTALAQALIHDPEIIILDEPT
                     SGLDPHQIIEVRELIRRLASGRTVILSTHILQEVEAAADRIVIINRGRIVGDGTLQEL
                     RERAKKHDRAEVSVRGERNEVERLLSGLEGTRRVHFIGETDGFVSFQLLGKPGSQQWR
                     EIGKLARLKGWELRELTERPLSLEETFLALTEPEALAKSEKGELG"
     gene            4434..>5542
                     /locus_tag="PKW60_08120"
     CDS             4434..>5542
                     /locus_tag="PKW60_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HPJ99434.1"
                     /translation="MKNIRTIISRDLGAYFTSPVGYIFIIVFLAMSVGIFMTSFFTFP
                     MADMRNFFANLPIILCVFIPAVTMRVWAEERKENTWELLLTFPMRARELVLGKYLATL
                     AFYLITLAATGTVPLMLAMLGNPDGGMIMGGYLGAALLGAMFLAMGLFFSGFCKDQIV
                     AFVITLLACFAVFLVGTNFIAGYIDGFFPGLGSALSQMAGVIDHYNAFIRGVVEVRDV
                     LYFVGWTALFLVLNALYIEGRSRPGIRVQFGAAIAICLGIGFALNWLTIGTSLYRFDL
                     TEDKIYTVSPAAKRILSQVKRPVQVNLYITPKSGMPTAYTQLEQDISGKLDELRVASN
                     GMIEPHVVYLQAANVMTSAPAFGEEEKEENQADKA"
BASE COUNT         1091 a         1622 c         1758 g         1071 t
ORIGIN      
        1 cggccgtgtg cattgaactg acgaagaaat ttgaagatgt ccggcgcggc gacctggcgg
       61 aattggcccg ggattttgcg cagaccaggg tgaagggcga gatcaccgtc gtcatcgccg
      121 gccaccatcc caaattcctc gctgcagaaa ccagtaccgg cgccgaatga cgggccaggc
      181 agcgctgccc ggatcgtgca atgccggtcc ttccgcaaga cagggcgttg cggcgccggc
      241 cggggacttc ttgctatact ctgcatcgtt tgcatgatgg caatgcatcg atatgccgcg
      301 ggagaacggt tgtggccaac atacttgtag tgggaagcgc caacattgat ctggtaaccc
      361 gggttccacg cgttccaatg ccgggagaaa cgctgctggg ccgctcgttc gatacggttc
      421 cgggcggcaa gggcgcaaac caggccaccg cggcggcccg gctcggggcg gcggcctggt
      481 ttgcgggctg cgttggcaat gacgcctttg gcgagatgca gcggcggaat ctcgcggccg
      541 aagggattga tctgacgtac ctgcaggtcc atccttccga accgacaggc acggccgtga
      601 ttctggtggc cgatgagggg caaaactcaa tcgtggtgac tccggccgcc aatatgggca
      661 tccggccgga ggatctcgcc gggatggcag cggtatttgc cttggttgat gccgtattgg
      721 tgcagctgga gattcctctg gacaccgtcg agtccgcgct ggacatggct cgccgccacg
      781 cggtgctgtc gattgtcgat gcaggccctg cgcagcagtt gccggacgcc atcattgaaa
      841 aggccgacgt actctcgcct aacgagaccg aggcggaagc gctcacgggc gtctcgatcc
      901 gatcgctcga cgatgcgcgg aaagccggcc gggagctgct ggagcgcggg gcgcggcgcg
      961 tggtgatgaa actgggcgcc atgggcgcgc tctacatcga tgacaaagag tggattcacg
     1021 tgcctgcgtt ttcaatcacg ccggttgaca cgacggccgc gggcgacgca ttcacggcgg
     1081 cactcgcgct ggtctggcag gagcgccgcc cgggcgatgc gctcacgttc gccaacgctg
     1141 cgggcgcgct ggcggcgacg gtcgccgggg cccagccgag catgcccacg cgggccgcgc
     1201 ttgacgcatt tctgaaagca caaggaacgc attcgtgaac aaagatctgc tgtattcggc
     1261 gtcgattatg tgcgccaacc tgttgcgttt ggaaggcgag ctggccgcgc ttgaggccgc
     1321 gggttgcgat gagctgcatt ttgatgtgat ggatggccag tttgtgccca atatcacgct
     1381 gggcttcgac ttcatcaaag ccgtccgctc gtatacgagc cttccgtgca gtgcgcacct
     1441 catgatcgag cggcccgagc ggtatattga gcgcttcgtc gaagccggct gccacagtgt
     1501 caccgttcat gtagaggcct gcacccacgc gcaccgcgtg ctgaatcaga tcaaagcggc
     1561 gggcgcgaca tccggcatcg cgatcaatcc ggccacggca cttaccaaac tggactacct
     1621 cctggacagc gccgatcgtg tgctggtgat ggccgtggat ccagggttta caggacagac
     1681 tattctgaaa ggcactttcg accgcgtgcg catcctgcat gagaacctcc ggtaccggaa
     1741 gctggatgtt cgtatcgaag tggacggcaa catcacggtt cgcaatgcgg cgatcctggc
     1801 caatctgggt gcctcgatcc tggattgcgg cacatcaagc atcttcgacg ggcggcctcc
     1861 cggcgaggcc ctggccgact tccgggagaa agtcgcgctg gaacgcaaga tcgtgtgagt
     1921 ggcctcgtgc ccgatgagag cagtatccgg catccgggac gatttttccg caacggaggt
     1981 cttacggcat aaaatgagcc gctggcaggg tcagggcaac gcagaaaaat tcgttttgcg
     2041 cagcggcgat gggtaagagg ctcattcggc ccggccatgg ttgcgtgcag tgggcctgag
     2101 ttaaatgtca cgcgacgtgt tctgtgccgc agccgaatga gcctcgtggg aggctggaat
     2161 ttcatgcggg cattcggcga agtgtcaagc cgtcctggcg tgcgcgagtc tggacagccg
     2221 ctcgaaaccg tgcgggcccg accggaattg tcagcgcgcc tccttccaca gacgggacga
     2281 acttgacacc ccgctcgaat accaaacggt ctatcgaaaa ccgccagaca cggcgttctg
     2341 cgggccgtat ccagggcggg aatgccggct ccgctgcacc ggcgcacctt tcaacgcacg
     2401 aaccgtccat actacaattt gcacgatacc gccgttacag cgggtcctgg tgggcaccag
     2461 gccgctttcg ggtgaattac ttcgaatagc gtgaaacact tggaaaatgt ggatccgcgt
     2521 gctaagattc ctgcattacg tccggagtaa acccctgtct ggaacaagtg gatttcagga
     2581 cgcaaccgag agaggaagac cccaatgaag agactgttgg cgttagtgct ggtgggtgtt
     2641 tttatggtag ggatggccgg ctgcagcggc ggcgcgaaag atgcggcgcc cgcgggcagc
     2701 ggaatcggag aatccgttgc tccatcttcg gagggagcat ccactgaatt gtccgttgag
     2761 ccggaggaat ccgctgagga gacttcgaac gacacagcgg cagccgaatc cgcgggggga
     2821 accgagctga cgggaaccaa gtggcaattg ggcgattatg agatcaattt caaggacgat
     2881 ggcaaagtgc tcgtgaaagg cggtcagatc tccaccctga tgcccgatgg cgccgagggc
     2941 gaatacaaaa tagaagacgg caaattctcc gtcagcattc tcggccagtc cttcaatggc
     3001 acgtgggatg gctcgaagct cgtggtggat ggcaacgagg ccgttctgca ataaccagtc
     3061 cagaaaatca ttaaaccggc gcccaggatt atgggcgccg gtttttgttc ccgccacgca
     3121 tccatttgcc tgtccagtgt ccgtgcccgc ggcgctgctt catatggggc aggatgccgc
     3181 atatgggcat ctttgccgag ccgccggaat gccgtaaacg gcggggcgcc ggttgcaaac
     3241 ccttcaaaat caatgctatc gtagcgtctc ttttgtattt tcacgggcag gcacgatcat
     3301 tgctggcctt gcggcgggga tacctgcatg cgtccggtca gggcagcccg ctctatcagg
     3361 cgagacggaa cgcagcggcg ctgtacagcc ccaacagctg ctgaacaaac ggatcgagaa
     3421 ccgcgcccgg gtctgcggtg tgcgggatcc aggctcagga gtatttgcga catgatcaag
     3481 acacagaacc tcaccatgca ttacgggccc gtgcgggccc tggatcgggt gtcgttcgag
     3541 gtgaagcagg gcgagattgt cggcctgctc ggccccaatg gcgcgggcaa atcgacgacc
     3601 atgaagatcc tgacgaccta tcttcacccc accagcggca ccgccacggt cggaggcatg
     3661 gacgtgctcg aaaacccgct gggcgtgcgc aaggtcatcg gctacctgcc cgaggtgctt
     3721 cccctgtaca tggacatgga agtgcgctcg tacctcagtt ttgtggcccg gtcgcgcggg
     3781 ctgggcggcg caaaactccg tgagcgcacc gcggcggttc tggaggaatg cgggctgaag
     3841 cccatgtacc gcaaagtcat acgcgagcta tccaaagggt accgccaacg gacggccctg
     3901 gcgcaggccc tcatccacga cccggagatc atcattctcg acgaaccgac ctccggtctc
     3961 gatccgcacc agatcatcga ggttcgcgaa ttaatccggc ggcttgccag tggccggacc
     4021 gtgatcctct ccacccacat tctgcaggaa gtagaggcgg ccgcagaccg gatcgtcatc
     4081 atcaaccggg gccgcatcgt gggcgacggc acgctccagg aactgcgtga acgcgccaag
     4141 aaacacgacc gcgccgaggt ttccgttcga ggcgagcgta acgaggttga acgtctgctg
     4201 tcgggcctcg aaggcacgcg ccgcgtacat ttcatcgggg aaaccgacgg tttcgtatcg
     4261 tttcaattgc tgggcaaacc ggggtcgcag cagtggcgcg aaatcggcaa gctggcccgc
     4321 ttaaagggtt gggaactgcg cgaacttacc gagcgcccgc tgagcctgga ggaaaccttt
     4381 ctggcgctca cggagcccga agccctggcg aagagcgaaa agggagagct gggatgaaga
     4441 acatcagaac gataatctcg cgcgacctgg gagcctattt tacgtctccg gtcggctaca
     4501 ttttcatcat tgtgtttctc gcgatgagcg tggggatctt catgacgtcg ttcttcacgt
     4561 tccccatggc ggacatgcgc aatttcttcg ccaacctgcc catcattctg tgcgtgttca
     4621 tcccggcggt gaccatgcgg gtctgggcgg aagagcgcaa agagaatacc tgggagcttc
     4681 tgttgacctt tcccatgcgc gcgcgcgagc tcgtgctggg aaaatacctt gcgaccctcg
     4741 ccttctacct gatcacactg gcggccacgg gaacggtgcc gctcatgctc gcgatgctgg
     4801 gcaatccgga tggcggcatg attatgggcg gctacctggg cgcggcgctt ctcggggcca
     4861 tgtttctggc catgggcctg tttttctcgg gcttctgcaa agaccagatc gtggcgttcg
     4921 ttataaccct gctcgcgtgc tttgccgtgt ttcttgtcgg cacgaacttc attgcgggct
     4981 atatcgacgg gtttttcccc ggcctgggtt ccgccctgag ccagatggcc ggcgtgatcg
     5041 accactacaa tgcctttatt cgcggcgtgg ttgaagtgcg cgatgtgctg tatttcgtgg
     5101 ggtggacagc cctgtttctg gtgctgaacg ccctgtacat cgaagggcgc agccggccgg
     5161 gcatccgcgt gcagttcggc gcggccatcg ccatctgcct cgggattggc tttgccctga
     5221 actggctcac catcggcacc agcctttacc ggttcgacct cacggaagac aaaatctaca
     5281 ccgtgtcgcc cgcggcgaaa cgcatcctgt cgcaggtcaa acgtcctgtg caggtcaatc
     5341 tctacatcac gcccaaaagc ggcatgccca ccgcctacac acagctggaa caagatattt
     5401 cgggcaaact cgatgagctg cgggtcgcgt ccaacggcat gatcgaaccc cacgtcgtgt
     5461 acctgcaggc ggcgaacgtc atgaccagcg ctcccgcgtt tggagaggag gagaaggaag
     5521 agaatcaggc cgataaagcc ga
//
LOCUS       DAOPBQ010000216         5518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12659_length_5518_cov_4.070474, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000216 DAOPBQ010000000
VERSION     DAOPBQ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12659_length_5518_cov_4.070474"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PKW60_08125"
     CDS             <1..843
                     /locus_tag="PKW60_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPJ99435.1"
                     /translation="GVNYFDTADGYGQGRAETVLGACLADYPRHELFVLTKVGANWTE
                     GPNSGGLSAKHIRESCDESLRKLRMDYVDVLMCHRPDPGTPLEETVRAMDDLIHAGKI
                     LYWGVSNWPGGLIAKTNAIARGLGAREAAANEPRYNLVYRYPESQIFPAARAEGMGNF
                     TFSPLGHGLLAGIYKPGEAPPAGSRAAVDGQNPVTRGLYFREDRIAKAQEFTRIADEF
                     GVSAAELAIAWVLHQPDVTSCIVGAWRPEELLLDLKAASLTLPPDMLATLDALFPSPA
                     IPSL"
     gene            1012..1908
                     /locus_tag="PKW60_08130"
     CDS             1012..1908
                     /locus_tag="PKW60_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99436.1"
                     /translation="MIIVLLRNIMGKKCVHSLLIGLLVMLGLSVSAIAQPTVERVSET
                     PVAYVKKPFEVRWEVRWHGASDAYTVLPPEIEDVDWAAVAPGPAEAFVRDGVNVVVQS
                     LRITPNQLGSFEVPEIRVGYLTPGELTPAKQPEEVTGTQEPEAQIPSLRLAGFTVEVR
                     RDLTVLWAALAISGAGALLYLGGMGLLVVQRRRTARSEAVTAADHPGQLADPRAAGVA
                     LHEAKRCRVDGDIYTFYLRLAQAAELLGSEEGELAGVLQARAQKVGFQGYAPTNDEME
                     TDYRAVARALKLSVHEDEIPVS"
     gene            1930..2916
                     /locus_tag="PKW60_08135"
     CDS             1930..2916
                     /locus_tag="PKW60_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPJ99437.1"
                     /translation="MALSVEAIRKEVEAQSRFVSRLRDQMARVIVGQQYMVDRLLVGL
                     LANGHVLIEGVPGLAKTTAVKTLAQAMNCSFSRIQFTPDLLPADLIGTLVYSPKDGNF
                     TTKKGPIFANIILADEINRAPAKVQSALLEAMQERQVTIGDTTYPLEEPFMVMATQNP
                     IEQEGTYPLPEAQVDRFMLKLRVNYPSRVEEREIMDRVDLLHAPEVRPAVTREEILRA
                     QALVNRIYVDEKAKDYIVNIVQATRYPEAFELNINHLIEYGASPRATIYLQQAARALA
                     FLQGEGNVFPNDVKQVAMDILRHRIIVTYEAEAENMTSEDIIRQILDTVPVP"
     gene            2936..3814
                     /locus_tag="PKW60_08140"
     CDS             2936..3814
                     /locus_tag="PKW60_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPJ99438.1"
                     /translation="MIPQEVMQQIRRIQIRTSHMVNDILAGQYESVFKGQGMEFREVR
                     EYVPGDDIRMIDWNVTARTGVPHVKLLAEERELTVMLMVDASGSERFGGVGRLKIELA
                     AELCAVLAFSAIQNNDKVGLIVFTNDVELYVPPSKGRRHVLRVIREVLHFQPRGRRTN
                     IPGALHFLNGVTRRRAVTFLVSDFMAERYEVPLRVANRRHDVIAVHVSDPREEVLPDV
                     GLVAVKDAETGQESLVDTSVRSVREGYARAARERTKTRDDIFRRTGVDVINVRTDQSY
                     VNELYRFFRMRERRYA"
     gene            3839..5128
                     /locus_tag="PKW60_08145"
     CDS             3839..5128
                     /locus_tag="PKW60_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPJ99439.1"
                     /translation="MGENTPERLKAFREEARERSRQGRLWPLINLIVLVLGFAGVLAA
                     VVWLPAHGGAARQTGERGGLSDAALRDYATLLEHKGLKDAALHAYESYLERAFLEPGA
                     RANVCYSIGKLAISMERYEKALEYLYQAEMLNPENPVSDEISSKITLCLERLGRSNDL
                     RRELRKRTALQRTADDLEEGEVVLAEFAGQIITDRDLDREIEKLPPAARDSFAAPDKR
                     IELLRNMVAERLLLDKALRLELDQTPEVQDELARLRDSLVVQTLIDQEIDSKISISPE
                     DIKRFYDAGLERFTEPAVRTGLEGSGESADAARLNLEEAQKNPDGATKVRVSGGRILQ
                     GLGKSELDEAVLDVIQKTAPGSVSAPVELDGVWYVFAVSETPARVHPFEEVKAQAERQ
                     LRAEKQREQFRALVADTLKARNVQLYPERLKKEPSEE"
     gene            5125..>5518
                     /locus_tag="PKW60_08150"
     CDS             5125..>5518
                     /locus_tag="PKW60_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99440.1"
                     /translation="MSLIASIAWLVAAASAPEVNVRVVVEPPVIPFHRQAEYRIEVEA
                     PADANVSIPEMAGKFGGLAVYGPPVREREEIRGGRVKIREIYVLDPIFMGYYPIAPAV
                     VKVDEAQELSVPSPAIRVRDLTPEERMEA"
BASE COUNT         1140 a         1569 c         1785 g         1024 t
ORIGIN      
        1 ggggtcaatt acttcgacac ggccgatgga tacggtcagg gccgcgcgga aaccgtgctc
       61 ggcgcgtgtt tggccgatta cccgcgccac gaattgttcg tgctcaccaa agtgggcgcg
      121 aattggacgg aaggcccgaa cagcggcggc ctgtcggcca agcatatccg tgaaagctgc
      181 gacgagagtc tgcgcaaact ccggatggac tacgtcgatg tgctgatgtg ccaccgcccc
      241 gaccccggca cgccactcga agaaacggtc cgggccatgg acgatctgat tcacgcgggc
      301 aagattttgt actggggcgt gagcaactgg cccggcggcc tgatcgcaaa aaccaatgcc
      361 attgcgcgtg gtctgggtgc gcgggaagcg gccgccaacg aaccacgcta caacctcgtg
      421 taccgctacc ccgaatccca gatatttcca gcggcgcgcg ctgaaggcat gggcaatttt
      481 acgttttcgc ctctgggcca cggcctgctt gcgggcatat acaagccggg tgaagcgcca
      541 cccgccggtt cgcgggccgc ggtggacggg caaaacccgg tcacacgcgg actctatttt
      601 cgtgaggacc gcatcgcaaa agcgcaggaa ttcacccgaa tcgccgacga attcggggtt
      661 tccgccgcgg aactcgccat tgcctgggta ttgcaccagc ccgatgtcac gagttgcatc
      721 gtgggcgcgt ggcggcctga ggaactgctg ctcgacctca aagccgcgtc gctgaccttg
      781 ccgccggata tgctggcgac actcgacgcg ctgtttccat cgcctgcgat tccgtctctt
      841 tagcagtttg agcaggcgga agcagccgga gacctatgct ccgcagctcg attgatatgc
      901 cccagatgag tcacgccccg ctctattgcc tcaaatgagg gaaggcaggc ccgggaatgg
      961 aaggattaac tcatagtcaa caaatagttt acgccggaag gaatctgcgg catgataatt
     1021 gttctcctaa gaaacattat ggggaagaaa tgcgtccata gcctgttgat cgggttgttg
     1081 gtgatgctgg gcctttcggt ctcggccatc gcgcagccaa cggtcgaacg cgtctctgag
     1141 actcccgtgg cctatgtcaa gaaaccgttt gaggtccgct gggaggtccg ctggcacgga
     1201 gcgtccgatg cgtataccgt gcttccgccg gagatagagg atgtggattg ggcggcggtt
     1261 gcgcccggcc cggccgaggc atttgttcgc gatggcgtca atgtggtcgt gcaatccctg
     1321 cggatcacgc ccaatcaact cgggtcgttt gaagttcccg aaatccgggt gggctatctg
     1381 accccgggcg agctcactcc ggcgaagcag cccgaagagg ttaccggaac tcaggagccc
     1441 gaggcgcaaa ttccgagctt gcgcctggcc gggttcacgg ttgaagtccg cagagacctc
     1501 acggtactgt gggcggctct ggccatcagc ggggctggcg cgctgctgta tctgggcggc
     1561 atgggcctgc tcgtggttca acggcgccga actgcccgtt cggaggccgt gacagcagcg
     1621 gaccaccctg gacaattggc ggatccgcgc gccgcgggcg tcgcgttgca tgaggcaaag
     1681 cgctgccgcg tcgatggaga tatttacacc ttctatcttc gcctggcaca ggctgccgag
     1741 ctgctcggaa gtgaagaggg agagcttgcg ggcgtgctcc aggcccgcgc acagaaggtc
     1801 ggctttcaag gatatgcgcc gacgaatgac gagatggaga cggactaccg cgcggtggcg
     1861 cgcgcgttga aactgtcggt tcacgaagac gaaataccgg tttcctaaga ggtgggaggt
     1921 atccaataca tggcactgtc tgtcgaagca attcgaaaag aggtggaggc gcaatcccgg
     1981 ttcgtgtcgc ggttgcgcga tcagatggcc cgcgtgatcg tgggccagca gtacatggtg
     2041 gaccggttgc tggtcggcct gctggccaac gggcacgtgt tgatcgaagg tgtgcccgga
     2101 ctcgccaaga ccaccgccgt gaaaacgctg gcgcaggcca tgaattgttc tttcagccgg
     2161 attcaattca cgcctgatct gctccctgct gatctcatcg gcacgctggt ctatagcccg
     2221 aaagacggga atttcaccac gaaaaagggg ccgatcttcg cgaatatcat ccttgccgac
     2281 gaaattaacc gggcgccggc caaggtgcag agtgcgctcc tggaggcgat gcaggaacgg
     2341 caggtgacca tcggtgacac gacctatccc ctcgaagagc cgtttatggt gatggctacg
     2401 cagaacccca tcgaacagga gggcacctac ccgttgccgg aggctcaggt tgaccggttc
     2461 atgctgaaac tgcgcgtgaa ttatcccagc cgggttgagg agcgcgagat catggatcgc
     2521 gtggatttgc ttcacgcgcc cgaagtgcgc cccgccgtga cgcgcgagga gatcctgcgg
     2581 gcccaggcgc tcgtgaaccg gatctatgtt gatgagaaag ccaaggatta tatcgtcaat
     2641 atcgtgcagg ctacgcggta tcccgaggcg tttgagctga acatcaacca tctcatcgaa
     2701 tacggcgcat cgccccgcgc gacgatatat cttcagcagg ccgcccgggc gctggcgttt
     2761 ctgcagggcg aaggcaacgt ctttcccaac gatgtcaaac aggtggcgat ggacatcctg
     2821 cgccaccgca tcatcgtgac ctacgaagcc gaggccgaaa acatgaccag cgaagacatc
     2881 atccggcaga tcctcgatac cgtgccggtg ccgtaagccg ggaagtaaaa ccgatatgat
     2941 tccgcaagag gtaatgcagc agattcgccg gatccagatc cggacgagcc acatggtgaa
     3001 tgacatcctc gcgggccagt acgaaagcgt attcaagggc caggggatgg agtttcgcga
     3061 agtgcgcgaa tatgtgccgg gcgacgacat ccgcatgatc gactggaacg tgaccgcgcg
     3121 cacgggcgtg ccccatgtga agctgcttgc cgaagagcgt gaattgacag tcatgctgat
     3181 ggtcgatgcg agcggttcag aacgtttcgg cggtgtggga cgcctcaaga tcgagctggc
     3241 ggccgaattg tgcgcggtgc tggcgttttc ggccattcag aacaacgaca aggtcgggct
     3301 catcgtattc acaaacgacg tggagctcta tgtgccgccc agcaaaggca gaaggcacgt
     3361 actgcgtgtg attcgcgaag tgttgcattt tcagccgcgg ggccggcgaa ccaacatccc
     3421 cggggcgctc cattttctca acggcgtgac ccggcgccgg gcggtgacct tcctcgtctc
     3481 cgatttcatg gccgagcgct atgaggtgcc gttgcgtgtg gcgaaccggc ggcatgacgt
     3541 gatcgcggtc cacgtgagcg atccgcgcga ggaagttttg cccgatgtcg ggctggttgc
     3601 cgtgaaagac gcggagaccg ggcaggagtc cctcgtcgac accagcgtcc gcagcgtgcg
     3661 cgaagggtat gcccgcgcgg cgcgggagcg aacgaaaaca cgggatgata tcttccgccg
     3721 gacaggcgtc gatgtcatca acgtgcgcac cgatcagtcg tatgtaaacg agctctaccg
     3781 gtttttccgc atgcgcgagc ggcgctacgc ctgatgaagc aagatgagga taacggacgt
     3841 gggtgagaac acaccggaga gattgaaggc gtttcgagaa gaagcccggg aacgttcgcg
     3901 gcagggccgc ctgtggccgt tgatcaacct catcgtgctc gttctcggct ttgccggtgt
     3961 gcttgccgct gtcgtctggc tgcctgctca tggcggtgca gcgcggcaga ccggcgagag
     4021 aggagggctg tcggatgcgg ctctccggga ctatgcgacc ctgctggagc acaaagggct
     4081 taaagacgcc gccctccacg cctatgaatc gtatctcgaa cgtgctttcc ttgagcccgg
     4141 cgcccgggca aacgtgtgct acagcatcgg caagctggcg atcagcatgg aacgttacga
     4201 aaaagccctc gaatacctgt accaggctga aatgctcaat ccggaaaacc ccgtgagcga
     4261 cgagatcagc agcaagatca ccttgtgcct cgaacgactt ggccgctcca acgacctgcg
     4321 ccgggaactg cgcaaacgca ccgcgcttca gcggacagct gacgatctcg aggaggggga
     4381 agtggtgctg gcggagttcg cgggccagat tatcacggac cgcgatctgg accgcgagat
     4441 cgaaaaactg ccgcccgcgg cgcgtgattc ctttgctgct ccggataaac gaatcgagct
     4501 gctccggaat atggtcgccg aacgattgct gcttgacaag gcgctgcggc tcgaactgga
     4561 ccagacgccc gaagttcaag acgaactggc ccgcctgcgc gacagtctgg ttgtccagac
     4621 cttaatcgat caagagatcg attcaaaaat ctccatcagt cccgaggaca tcaaacggtt
     4681 ttacgatgcc gggcttgagc gttttaccga acccgccgtg cgcacaggtc tggagggctc
     4741 gggagaaagc gccgatgccg cccgcctgaa cctggaagag gcacagaaaa atccggatgg
     4801 cgcgacaaag gtgcgtgtga gcggtgggcg gattttgcag ggacttggca agagcgaact
     4861 cgatgaggcc gtgctcgacg tcattcagaa gactgcgccg ggatcagtgt ctgcacctgt
     4921 cgagctggac ggcgtatggt atgtgtttgc cgtcagcgaa accccggcca gagtccaccc
     4981 cttcgaagag gtgaaagcgc aagccgaacg ccagttgcgg gcggaaaagc agcgggagca
     5041 gttccgggcg ctggtggcgg atacgctcaa ggcgcgcaat gtgcagctct atcccgagcg
     5101 cctgaagaag gagccctcag aggaatgagc ctgattgcct ccatagcgtg gctggtcgcg
     5161 gcagcgtctg cgccggaggt caatgtgcgc gtggtggtcg agccgcccgt gatcccgttc
     5221 catcgccagg cggaataccg gatcgaagtt gaagcgccgg ccgatgcaaa cgtgtcaatt
     5281 ccggagatgg ccggcaagtt tggaggactg gccgtgtacg ggccgcccgt gcgcgaacgt
     5341 gaggagatac ggggcggacg ggtaaaaatc cgcgagattt acgttctgga ccccattttt
     5401 atggggtatt atccgatagc gcccgctgtg gtcaaagtgg atgaagcgca ggagctcagt
     5461 gttccgtcgc cggccattcg tgtgcgcgat ttaacccccg aagagcgaat ggaggcgg
//
LOCUS       DAOPBQ010000217         5479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12762_length_5479_cov_3.939712, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000217 DAOPBQ010000000
VERSION     DAOPBQ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12762_length_5479_cov_3.939712"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKW60_08155"
     CDS             <1..226
                     /locus_tag="PKW60_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99441.1"
                     /translation="NLGDTMLKSPIVESGPQTASQNAGAALPIEITHPVNLGKTTANS
                     SSKCTQGDFPFLKEDFIACSVSEAFLGPVV"
     gene            791..4135
                     /locus_tag="PKW60_08160"
     CDS             791..4135
                     /locus_tag="PKW60_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPJ99442.1"
                     /translation="MRKAMVALSILMAAGGAWASGGVRPALDLDGAWEFRLDPASVGE
                     AEGWNSGSASFPDAIQVPGAWDAQGFGGETPKMHHSHVGKGWYRRTVDIPADWQGRRL
                     FLCFGGVYRSAKVWVNGEFAGAHLGYVSDFEFEITPHAVAGQPCRIVVLVDSEQRWTE
                     DALQGCMDIIDHLFTYWGGIWSHVTLESRSTAWLCDVFVEPRTDPAGCVVQAVVAGDS
                     SLPRRAEVAIFESDGAPIASRRQDIGPDGTIRVDLELPGARLWTPEEPHLCTAQIVLS
                     DEACSPIDETRTRFGLRTIEIRDSDFYVNGVKYYLNGYGDDCVYPDTIAPPADKQLYL
                     DRLRVAKSYGFNFVRHHSHFVAPEYYEACDEVGMFVSPELPIAYPRFYNRAHGAALEL
                     YASEWSAVIKRYRNHPSIFDWCMANELWEGMPRIGPELYRIAKELDPTRPVIDSDGIF
                     AGGFADGTKDRPTLDFYAIMFDILTTPLDNPDKFTTGTPRKPIITHEEGNFVHFPLFD
                     EIELFGDTIKPLWITDCRDRVARAGLLDEAPAWSKSSQQLYLLCHKANIEALRKNPCI
                     SGYTWWLLQPWYCGSNGLLDVHRRPISVTPGQVRRFNAPAVLLQDGLRQTYAGGDVIT
                     VEITLSNYSGKAVEPGRLTCTLMHDDQILDSRVIQSGAVPNGSVEALGEAEFTLPDAA
                     EPQRLSLQAALEDEEEPLCNDWTLWVYPDPPPAAPPRTPVYASPDTMALLAAFQPQAL
                     PDGARLPSRAVYVARQPSEALVAAAERGSCVVLLSPAGVFPTDCTTFKSAWWLGVFDG
                     DSNAGTYVYDNPVTRGLTPDHWCDASWFHLLQGAQTLLLDDLPAQPEVLIRALNTHGA
                     PHPFSQYLDFEYVWRNKAVLAQARVGKGSIIISGLNFDVAARNGGPEAAYVLSRLIEH
                     ACELPRPDAKWSGRFLKSAIQSSAFANAPLVSGFTRLRYHKGVETQAQSYRERSATAL
                     QIRQEEPLHRLEWESAPMPGAPKVTFVFAGGFPFMEPPLTNPGFTLAVNGQPLVDFDT
                     SKTQTQWPGRDGRSALLYVPGHVQPSWSYTAGLFYLTVPRDVLVPGQPCRLEVRSRGS
                     DNRRYFNLNPYTDIFFEPQSQPWRSE"
     gene            complement(4221..>5479)
                     /locus_tag="PKW60_08165"
     CDS             complement(4221..>5479)
                     /locus_tag="PKW60_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99443.1"
                     /translation="AYTDFVQSFLIIVLSVMLLPAGLSRVGGFAGLHESLPPHFFTLY
                     SEVSGLTAFTIAMLTVNGIVGIVAQPHMLSLSATGNTERAGRIGQTYGTFVKRICTIG
                     WALTGLIVAALVVQQGMELPEGEAAFGFACREFLAPGLTGLMIACILAANMSTCSNFM
                     VNTGALFTRNFYREYINPGASDRQLLMMGRFSGLALTLLGILFAVAVERVLDAFLFTE
                     TIAALMGVMFLGGMLWKRANRCGAWAATLGAFLSYYALNYLMSCRAGADSAAFSTLSD
                     AFAELVERTRAGEMAAFLNTGVYKLVYPWKPGPFGWAMVVGFTALIIGSLLTRPEDGE
                     LIEQFFDKQRRTTDEEPDAETGLRPLAAARGEELVLLDLPGWFTAERWQGFFKRYRED
                     LVGFVLAWFTVGFLLALAWGILQIGK"
BASE COUNT         1078 a         1742 c         1609 g         1050 t
ORIGIN      
        1 gaatctcggg gacacaatgc tgaaatctcc catcgtggag agcgggccgc agaccgcttc
       61 gcagaacgct ggagcggcgc ttcctatcga aataacccat cctgtcaatc ttggcaaaac
      121 aacggccaat tcaagttcta agtgcacgca aggcgatttt ccgtttctga aggaagattt
      181 cattgcgtgt tcggtatccg aggcgttttt ggggccggtt gtttaattta acgactatct
      241 ccgggaattt ttctaatgta tgggcaggtc tctgtgcctg cccatccgga tagcgcctgc
      301 attgcgacgg gacgctgtga gctgcaagct acagccaaaa tccaacctgc aaaactccac
      361 ccccaaatcg gctaccgccg attcaccccc gcccgcgggg gacaggtacc ctgggtgcgc
      421 cggcggcctc gtcggcacac ggctcgccct cgcccgcggg ggacaggtac cctgggtgcg
      481 ccgacggctt cgtcggcaca cggctcagcg tcgcccgcgg aaaccagtgc ccgcgcaggc
      541 gcaatatggg tcccgatgtc tttttcttct ccatctcctc gctcaaaact cacccacagg
      601 aaaagggcca aagccaaaaa ccgtgactta gcattagaat gcgcgtccgt tcccgcagcc
      661 atcggctcgc cctccccgcg aatcttgtga attcagggga cgccatacta aattatctcc
      721 atcgcaaaga atcgtcgccg gccagtcaag aaaatatttc agtagagagt cctcgaaata
      781 aggaggtccc atgcgcaagg caatggttgc gttgagtatt ctgatggctg cgggcggcgc
      841 ctgggcctcg ggcggcgtgc gtcctgcgct cgatctcgat ggagcgtggg agttccggct
      901 cgatccggcc agcgtgggtg aggcggaagg ctggaattcc ggctccgcct cgttcccgga
      961 tgcgatccag gtgcccggcg cgtgggatgc tcaggggttt ggcggggaaa cgcccaaaat
     1021 gcaccacagc cacgtgggaa agggctggta ccgccgcacg gtcgatattc ccgcggattg
     1081 gcagggccgg cgcctctttt tgtgttttgg aggcgtgtac cgatccgcaa aagtctgggt
     1141 caacggggag tttgcgggcg cccatctcgg gtatgtgtcc gatttcgagt ttgaaatcac
     1201 cccgcatgcg gtcgcgggcc agccctgtcg catcgtggtc ctggtggact cggaacagcg
     1261 atggactgag gatgcgcttc aggggtgcat ggacatcatc gaccatcttt tcacgtactg
     1321 gggcgggatc tggagccatg tgacgctcga atcccggtcc acggcctggt tgtgcgatgt
     1381 ctttgtcgag ccgcgaacgg atccggccgg ctgtgtggtc caggctgtgg tggcgggtga
     1441 ttccagcctt ccgcgccggg ccgaagtcgc tatctttgag tccgacggcg ccccaattgc
     1501 atccaggcgg caggatatcg gcccggacgg cacaatccgg gtggatttgg aattgcctgg
     1561 ggcgcggcta tggactcccg aagaacctca tctgtgcacc gcgcagatcg tgctgagcga
     1621 cgaggcctgc tcgccgattg atgagacccg gacccggttt gggctccgca ccattgagat
     1681 ccgtgattca gatttctacg tcaacggcgt gaagtactat ctgaacggct atggcgacga
     1741 ttgcgtatac cccgacacga tcgcgccgcc cgccgacaag cagctctacc tcgaccggct
     1801 ccgggtggcg aaatcgtacg gattcaactt tgtcaggcat cacagccatt ttgtcgcgcc
     1861 ggagtactat gaggcctgtg atgaagtggg gatgtttgta tcgccggagc ttccgatcgc
     1921 ctatccccgg ttttataacc gtgctcacgg cgcggccctg gaactctacg catcggaatg
     1981 gagcgcggtc atcaaacgct accgcaacca cccctccatc ttcgactggt gcatggccaa
     2041 tgagttgtgg gaaggcatgc ctcggattgg cccggaactc taccgcatcg caaaagaact
     2101 ggacccgacg cgcccggtga tcgattcaga cggcatcttc gccggaggct tcgccgacgg
     2161 cacgaaagac cgccccacac tcgattttta cgccatcatg ttcgacatcc tgaccacgcc
     2221 gctcgacaac cccgacaaat tcacaaccgg cacgccccgc aagcccatca tcacccacga
     2281 agaaggaaac ttcgttcatt tccccctttt tgacgagatc gagcttttcg gggacacgat
     2341 caaaccgttg tggatcaccg attgccggga tcgcgtcgcg cgcgccggcc tgctcgatga
     2401 agcgccggcc tggtcgaaat cgtcgcagca gctctatctc ctctgccaca aggccaatat
     2461 cgaggccctg cgcaagaatc cctgcatttc cggctacacc tggtggctcc tgcaaccctg
     2521 gtactgcggt tcaaacggcc tgctggacgt tcatcgccgg cccatctcgg tcacccccgg
     2581 gcaggtgcgg cgcttcaatg cgcccgcggt gttgctccag gacggcctcc ggcagaccta
     2641 tgccggcggc gacgtgatca cggtggagat cacactgtcc aattattcgg gaaaggcagt
     2701 cgagccgggc cgtctcacct gcacgctcat gcacgatgac cagatcctgg actcgcgcgt
     2761 gatccaaagc ggcgcggtgc caaacggcag cgtggaagca ctgggtgagg cggagttcac
     2821 tctgcccgat gccgctgaac cacaacgcct gagcctccag gccgccctgg aagacgagga
     2881 agagccttta tgtaatgact ggacgctgtg ggtataccct gatccgccgc ctgcagcgcc
     2941 gccgcggact ccggtgtatg caagcccgga caccatggcc ctcctcgctg cgtttcaacc
     3001 gcaggcgctg cccgatggtg ctcggctccc gtcgcgggct gtctatgtcg cccgccagcc
     3061 gagcgaagcc ctggtggctg ccgctgaaag aggaagctgc gtggtgctat tgtctccggc
     3121 cggcgttttc ccgacggatt gcaccacgtt caaatccgcg tggtggctgg gcgtatttga
     3181 tggcgacagc aacgcgggca cctacgtcta cgacaatcct gtcacccgtg gcctgacacc
     3241 ggaccactgg tgcgacgcct cctggtttca tttgctgcag ggcgcccaga ccttgctgct
     3301 tgacgatctt cccgcccagc cggaggtcct gatccgggcg ttaaacaccc acggcgcccc
     3361 tcatccgttc tcccaatacc tcgatttcga atacgtctgg cgcaacaaag ccgtgctcgc
     3421 ccaggcgcgg gtgggcaaag gttcgatcat catcagcgga ctcaattttg atgtggcggc
     3481 ccgcaacggc ggtcccgagg ccgcctatgt gctctcgcgg ctgatcgagc acgcctgcga
     3541 gcttccgcgg ccggatgcga aatggtcggg ccggttcctg aagagcgcca tccagtcgtc
     3601 cgccttcgcg aacgccccgc tggtgtcggg attcacccgc ctgcgttatc acaaaggagt
     3661 cgaaacgcag gcgcaaagct atcgggaacg gagcgccacc gcgctgcaga ttcgtcagga
     3721 agagccgctc caccgcctgg aatgggaatc agcgccgatg cctggagccc caaaagtgac
     3781 gttcgtgttt gcggggggct tcccgttcat ggaacctccg cttaccaacc ccggcttcac
     3841 gctggccgtc aatgggcagc ccctggtcga tttcgacacc tcaaagacgc agacccagtg
     3901 gccgggccga gatggccgct ccgcgctcct gtatgtgccc gggcatgtcc agccttcgtg
     3961 gagctacacg gcgggactgt tttacctcac agttccccgc gacgtgctcg tgcccggcca
     4021 gccgtgccgg ttggaagttc gctcgcgcgg cagcgataac cggcgctact tcaatctgaa
     4081 cccgtatacc gatattttct ttgaaccgca gtcccagccc tggcggtctg aatagagtgc
     4141 aagggtattt taagagagac tggcgagatt tcatccggtt gtgccaggtt caggacaatt
     4201 tgcaattgcg gcggcagcgt ctatttcccg atctggagga tgccccaggc cagggcgagt
     4261 aagaacccga ccgtgaacca ggcgagcaca aacccgacga ggtcctcgcg gtagcgtttg
     4321 aagaagccct gccagcgttc ggcagtaaac catccgggga gatccagcag gaccagctct
     4381 tcgcctcgag ctgcggccag cgggcgaagg ccggtttcgg cgtcgggctc ttcgtcggtt
     4441 gttcggcgct gcttatcgaa aaactgttca atcagctcgc cgtcttcggg acgcgtcagg
     4501 aggctgccga ttatcagcgc ggtaaatccc accaccatcg cccagccaaa gggtcccggc
     4561 ttccaggggt agaccagctt gtagactccg gtgttcaaaa atgcggccat ctctccggcc
     4621 cgggtgcgct caaccagctc ggcaaatgca tctgacagcg tcgaaaatgc cgcggaatcc
     4681 gctccggccc ggcaggacat caaataattc agcgcgtaat agctcaaaaa tgcgccgagc
     4741 gtggccgccc acgcgccaca gcggttggcg cgtttccata acatgccccc aagaaacatc
     4801 acgcccatca gggcggcgat cgtctcggtg aataaaaacg cgtccaggac ccgctcaacg
     4861 gccacggcaa agaggattcc cagcaacgtc agcgccagac cggaaaaacg gcccatcatc
     4921 agcaattgcc tgtcactcgc gcccggattg atgtattcgc ggtagaaatt ccgcgtgaac
     4981 agcgcgccgg tgttgaccat gaagttcgag cacgtcgaca tgttcgccgc gaggatgcac
     5041 gcgatcatca gcccggtcag ccccggcgcc agaaactcgc ggcaggcaaa cccgaatgcc
     5101 gcttcgcctt ccggaagctc catgccctgc tggacgacca gcgccgccac gatcaatcca
     5161 gtcaacgccc acccaatcgt gcagatccgt ttaacaaagg tgccgtaggt ctggccaatg
     5221 cgccccgctc gctcggtatt gccggtcgcc gacaaggaca gcatgtgcgg ctgggccaca
     5281 attcccacaa tgccgttgac cgtgagcatg gcaatggtga atgccgtcag cccgctgact
     5341 tcgctgtaca gcgtaaaaaa atgcggcggc agactctcgt gcagtcccgc aaacccgcct
     5401 acccgcgaca aaccggccgg cagcagcatc accgaaagca cgatgataag aaacgactgc
     5461 acgaaatccg tgtacgccg
//
LOCUS       DAOPBQ010000218         5476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12772_length_5476_cov_3.722007, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000218 DAOPBQ010000000
VERSION     DAOPBQ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12772_length_5476_cov_3.722007"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PKW60_08170"
     CDS             complement(<1..961)
                     /locus_tag="PKW60_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPJ99444.1"
                     /translation="MRHYARIPRPVKAAALAVFMLLIAAVGTGYAEADEGIPWLTSLD
                     AAKEQAAAQNKLIFMDVFAEWCGPCKMLASETFTAPEVIKALEAYIPLKIDSDKQPQV
                     ASRYGVRGVPTLFVLTAQGTTIHEKSGFMPPAGFLEFLQAAQGRMEAIARLEEEVRQA
                     PDKVDKVLELAHTYLELGRTGDALALLEKAEAHLDAVDSDTTKGDYAFALGLAYLIEG
                     AYDKGVERLEALIAAHPEHAEAERVKEMIPRGKLFGAMARVDQGDYEAARAILTELQE
                     TSTDEQVTAFAGEVLERLEVLGRPAPAWTASWVDSKPASLEKMK"
     gene            complement(1207..2238)
                     /locus_tag="PKW60_08175"
     CDS             complement(1207..2238)
                     /locus_tag="PKW60_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99445.1"
                     /translation="MKNLICTCECGHRFQVSDSAWGMESVCGKCGRPIRVTPGNTRPL
                     DVGIPLEESDSGSSSEHCARCGRPFRGDWDKNTSAEGVICHICANQSGRTQDVRPAMS
                     AGDGPPHAAAPEPAARLSPPADSTLEADGAVLIDHDPDAVDDEEPSLGKRFENFRETP
                     AFRRGLWAAAISVMVLAVVAAFMKTPPPPDDAAVEAVRESTSGLRMFGPPEAWSTSEK
                     AVVRVTMVVLETVFLFVPTFAALFLALAFLNRLPAHGWLASAIHAGVVSTGMTVVLYL
                     GPRVIGIFWGLPGALLAYLLAAFFIRVIYGPGLRTAALFVGLRAGFSLLADLARLLVY
                     GALGSTLLD"
     gene            2443..4179
                     /gene="ilvD"
                     /locus_tag="PKW60_08180"
     CDS             2443..4179
                     /gene="ilvD"
                     /locus_tag="PKW60_08180"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPJ99446.1"
                     /translation="MSKSVSPGRKSRKQHGKLEKKWSSAVTQGVERAASRAMLHAVGF
                     SLADFGKSQVGIASTWSQVTPCNVHIDLLAREAALGVERAGGKPVIFNTITISDGISM
                     GTEGMKYSLVSREVIADSIETVAGCQAFDGLVAIGGCDKNMPGCLMAIARLNRPSVFV
                     YGGTILPGVYKGEDVDIVSVFEAVGRHAKGEMSDRELQEIEAKAIPGPGACGGMYTAN
                     TMASAIEALGMSLPNSSAQAAISKDKAEDCRRAGAAVMAMLETGLRPRDIMTRKAFEN
                     AITVVMALGGSTNAVLHLLAMAHTAGVKLNLDDFTRIGKRTPVLADLKPSGKHVMADL
                     VKIGGLTPLLRELLKAKLLHGDCMTVSGKTLAENLRAIKAYPKKQSVIRPFSDPVKKT
                     GHLVILRGNLAPGGAVAKISGHEGTRFTGTARVFGSEEKALKQILDGTVKKGDVIVIR
                     YEGPKGGPGMREMLSPTSAVMGKGLGNDVALITDGRFSGGSHGFVVGHITPEAQVGGP
                     IAIVENGDRITIDAVKHSITLDVPKKEIAARLKAWKAPKPHYTRGVLAKYASLVGSAS
                     EGAVTDAALTLP"
     gene            complement(4273..5025)
                     /locus_tag="PKW60_08185"
     CDS             complement(4273..5025)
                     /locus_tag="PKW60_08185"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPJ99447.1"
                     /translation="MASPLTKGKQAFVRYREERKEVTILEICDDTVTVTVPNGSLPAK
                     NLGVSLEIPTLEGTYCYHTHVATAPRADDDILLLRRTASVKRFDRRRTWRVPLHARTK
                     ASRLDGRRVFRAVVVDVSAEGALLFTAGAFEPGELITFQLYLPNELPHEICARVVRVK
                     ETVRDGQTVYTLGAAYHELSKPARRALTYYIWKRLLELFPTEVRGLFRRGSQNQIDRR
                     LGLIGTPDEENPADNHGAGVSGDSSEGQAEYS"
     gene            complement(5234..>5476)
                     /locus_tag="PKW60_08190"
     CDS             complement(5234..>5476)
                     /locus_tag="PKW60_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008821254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HPJ99448.1"
                     /translation="PIAVADLQETVAQLKKNGIGVLITDHNVRETLEITDRAYIISEG
                     RISVAGTPREIAENPVARRTYLGERFRMDFDSRPAE"
BASE COUNT         1041 a         1706 c         1716 g         1013 t
ORIGIN      
        1 ctttcatttt ctcgagcgaa gccggtttcg agtcgaccca ggaggccgtc cacgcggggg
       61 ccggccggcc caggacttcc agacgttcca gcacctcacc ggcaaacgcc gtgacttgct
      121 cgtcggttga cgtctcctgc agctccgtca ggatggctct ggcagcctca taatcgccct
      181 gatctacgcg tgccatggcg ccaaacagct tgcctcgcgg tatcatctcc ttgacgcgtt
      241 ccgcctcggc gtgctcggga tgggccgcga tcagcgcttc aagccgttcc acgcccttgt
      301 cgtaggcgcc ctcgatcagg tatgccagcc ccagtgcgaa cgcgtagtca cctttcgttg
      361 tgtcggaatc caccgcgtcc agatgggctt cggctttctc taacagagcc agggcgtcgc
      421 ccgtccggcc aagctcgagg taggtgtgcg cgagttcaag gactttgtcc actttgtcgg
      481 gcgcctggcg cacttcctcc tcgaggcggg caatcgcttc catacggccc tgcgccgcct
      541 gaagaaactc aagaaaaccc gccgggggca taaacccgga cttttcgtga atggtggtgc
      601 cctgcgccgt cagtacaaag agcgtgggca ccccgcgcac gccgtagcgc gacgcaacct
      661 gcggttgctt gtcagaatcg atcttcagcg ggatgtaggc ttcgagcgcc ttgatgacct
      721 cgggtgcagt gaaggtctcg ctcgccagca ttttgcaggg tccgcaccat tccgcgaaca
      781 cgtccatgaa aatgagcttg ttctgggccg cggcctgttc ttttgccgcg tcgaggctcg
      841 tgagccaggg gataccttcg tctgcctcag cgtacccggt gccgaccgcg gcgatcagta
      901 acataaacac agccagcgcg gcagctttca ccgggcgggg gatccgtgca tagtgtcgca
      961 tgtggtaacc tccagtgtgt ggtggaatct cgcaggcctc aacgtattcc atatcagaaa
     1021 ctcaacggtt tcggctgtcg gcgtattcca gctccggccc aaaaactctt gtgcgccaga
     1081 ggcggcagta tgacagaagg cgccggcagg tgcgagatct ttctggtcag cgcccgcggc
     1141 cggcgccgcc cgcctttgtt ccggaaatgt ccgaataccg cgggcattca gagcagggat
     1201 ttctcttcag tccagcaggg tcgaacccag cgcgccgtag accagcaatc gcgcgaggtc
     1261 tgccagcagg ctaaatcccg cgcgcaggcc cacaaagaga gccgccgtcc tcagccctgg
     1321 gccgtaaatg acacggatga aaaatgccgc gagaagatat gcgagcagtg cgcctgggag
     1381 accccagaaa ataccgatga cccgcggacc caggtacagc acgacggtca tgccggtgga
     1441 caccaccccg gcgtgaatcg cgctggcaag ccatccatgc gcaggaagac ggttgagaaa
     1501 agccaacgcg aggaacagcg ccgcgaacgt cggcacgaac aaaaatactg tctccagaac
     1561 aaccatcgtg acccgtacga cggccttttc cgatgtggac cacgcctcgg gcgggccgaa
     1621 catccgaagt ccgctggtgc tctcacgcac ggcttcgacc gcggcgtcat cgggtggcgg
     1681 gggcgttttc atgaaagcgg ccacgaccgc cagcaccatc acgctgatgg cagccgccca
     1741 gagccctcgc cggaacgcgg gcgtttcacg gaaattttcg aaccgtttcc ccaggctcgg
     1801 ctcttcgtcg tccaccgcat ccgggtcatg gtcgatgagg acagcgccgt ccgcctccag
     1861 tgtgctgtct gcggggggtg acagacgggc cgccggctcc ggtgcggccg cgtgcggggg
     1921 gccatctccg gcgctcattg cgggccgtac gtcctgagtc ctgccgctct gattggcgca
     1981 tatgtggcag atgacgccct cggcagaggt gtttttgtcc cagtccccgc gaaatggccg
     2041 gccacaccgg gcgcaatgct cggagctgct gccgctgtca ctttcctcga ggggaattcc
     2101 cacgtccaag ggacgcgtat tgcctggcgt gaccctgatc ggccgtccgc acttgccgca
     2161 gacgctctcc atcccccagg cgctgtcgga tacctgaaac cggtggccgc attcgcaggt
     2221 gcaaatcagg tttttcatag cccgatcata cacgctttcc gtggtctttg tggagcatcg
     2281 agaaaaggcg gcggccacgt gtcacatcgc gaacggggcg ggagctgtcc gaaggcaggc
     2341 ggccgggccg ggttctgcag gcgcaaaacc ggacatagaa tgaccccgtc gaatgttagg
     2401 ctttcgaaag tgaggtaaag tgaatcatcg tggagtggta ccatgtcaaa atcagtaagc
     2461 cccggccgga agagcaggaa gcaacacggt aaattggaaa agaagtggtc gtcggcggta
     2521 acgcaggggg tcgagcgggc cgccagccgg gccatgctgc atgccgtggg cttttcgctg
     2581 gccgatttcg gcaaatcgca ggtgggaatc gcctcgacct ggagccaggt gactccctgt
     2641 aacgtgcaca tcgacctgct ggcgcgtgaa gcagctctcg gcgtagagcg ggccggcggc
     2701 aagcccgtca tcttcaacac catcaccatc tcggacggca tctcgatggg taccgagggc
     2761 atgaaatact cactggtttc tcgcgaagtc atcgcggact cgatcgagac tgttgccgga
     2821 tgccaggcgt tcgacgggct ggtcgccatc ggcggctgcg acaagaatat gcccggctgc
     2881 ctgatggcga tcgcacggct gaaccggccg tcggtgtttg tctacggcgg caccatcctc
     2941 cccggcgtct acaaagggga ggatgtggac atcgtttcgg tatttgaggc cgttggccgg
     3001 cacgccaaag gcgaaatgag cgaccgcgag ttgcaggaga tcgaggcgaa ggcgattccg
     3061 gggccgggtg cctgcggtgg gatgtacacg gccaacacca tggcttccgc catcgaagcc
     3121 ctcggcatga gcctgcccaa cagctcggct caggccgcca tctcgaagga caaggcggag
     3181 gattgccggc gtgccggcgc cgccgtcatg gccatgctgg agactgggct gcggccacgg
     3241 gatatcatga cccggaaggc ctttgagaac gcgatcacgg ttgtgatggc attgggcggc
     3301 tcgaccaatg ccgtgctcca tctcctcgcc atggcgcata ccgccggggt caagctgaat
     3361 ctggacgatt tcacccgcat cggcaaacgg acgcccgtgc tggccgacct caagccgagc
     3421 ggcaagcacg tcatggcgga cctggtcaag atcggcgggc tgacgccgct gctgcgcgag
     3481 ctgttgaagg cgaaactgct gcacggggac tgcatgacag tctcaggcaa gacgctggcc
     3541 gaaaatttgc gcgcaatcaa ggcctacccg aaaaaacagt ccgtcatacg gcctttctcg
     3601 gatccagtca aaaaaacggg ccacctggtg atcttgcgcg gtaatctcgc gcccggcggc
     3661 gcggtcgcga agatctccgg acacgagggg acgcgcttca ccgggaccgc ccgcgttttt
     3721 ggctccgaag agaaggcgct gaagcaaatc ctcgacggta cggttaaaaa aggcgatgtc
     3781 atcgtgatcc gctacgaagg ccccaagggc ggccccggca tgcgcgaaat gctttctccc
     3841 acctcggccg tcatgggcaa agggctgggc aacgatgtcg cgctgatcac cgatggacgt
     3901 ttcagcggcg gatcccatgg gtttgtggtg ggacatatca ccccggaagc ccaggtcggc
     3961 ggccccattg ccatcgtgga gaacggcgac cgcatcacca tcgacgccgt caagcactcc
     4021 atcaccctgg atgtgccgaa gaaggaaatc gcagcccggc tcaaggcctg gaaagccccc
     4081 aaaccgcact atacgcgcgg cgtgctggcc aagtacgcca gtctcgtggg atccgcctcc
     4141 gaaggcgcgg tgaccgacgc agcgctgacg cttccctgaa cctgcgggcc acgcccgctc
     4201 ccggtcaaaa tacgggatag aagcggcgaa acacgcattt ccggctggaa atgcgggaat
     4261 gggcggcgta attcaggaat attcggcctg gccctcagag ctgtcgccgg agacgccggc
     4321 gccgtggtta tcggcgggat tctcctcgtc gggcgtaccg atcagcccca ggcggcgatc
     4381 gatctggttc tggctgcccc ggcgaaacag gccgcgcact tccgttggga acagttcgag
     4441 cagccgtttc cagatgtagt acgtcagcgc ccttcgtgcc ggtttggaga gctcgtgata
     4501 ggccgcgccc agggtatata ccgtctgccc gtcgcgcacg gtctctttca cacgcaccac
     4561 gcgcgcacag atctcgtggg gcagctcgtt cggtagatag agttgaaagg tgatgagttc
     4621 accgggctcg aaggcgccgg cagtgaaaag gagcgcacct tcggcgctca cgtcgaccac
     4681 cacggcccgg aacacgcggc ggccgtccag ccgcgacgct ttcgtgcggg catgcagcgg
     4741 aacgcgccac gtgcgcctcc ggtcaaaacg cttcacgctg gctgtccgtc tcagcagcag
     4801 gatgtcgtcg tcggcacgtg gcgccgtagc tacatgcgtg tgatagcagt acgttccttc
     4861 caacgtaggg atttccagtg acacgcccag atttttcgcc ggtaaggagc cgttcgggac
     4921 ggtgacggtc acggtgtcgt cgcagatttc cagtatggtt acctctttgc gctcttcccg
     4981 gtagcgaaca aacgcctgtt ttcctttggt cagcggtgag gccacgctta tctcctcgag
     5041 atccccatta gaaacactct aaatcgtacc acataccccg catttcccgc taaatccctc
     5101 ccgcggaccc gaattgcgga gctgaaacgc ccctgcgcac ggctgcgaag cgcagcggca
     5161 ccgattttgt cgcaaaatcg acccagagaa gccgatctgg ccgttttcca gcggtttgag
     5221 cggggcacgg tggctattct gcgggtctgc tgtcgaaatc catccggaac cgttcgccga
     5281 ggtaggtgcg acgggccacg ggattttcgg cgatttcacg gggggtgccg gccacgctga
     5341 tgcgtccttc gctgataata taggcgcgat cggtgatttc gagggtttca cggacgttgt
     5401 ggtcggtaat cagcacgccg atgccgttct tcttgagctg cgcgacggtt tcctggagat
     5461 cggccacggc gatcgg
//
LOCUS       DAOPBQ010000219         5474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12779_length_5474_cov_4.252814, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000219 DAOPBQ010000000
VERSION     DAOPBQ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12779_length_5474_cov_4.252814"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKW60_08195"
     CDS             <1..298
                     /locus_tag="PKW60_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99449.1"
                     /translation="SLLDSLIPEDDGVPTSFFDAGVASDTDLDDDDKDLPVVNIADDN
                     TDSEGGMGGFASFSPGEPADAPAGPAMELGPRAADSQRGSQELKSPAPEEDAAS"
     gene            295..1050
                     /locus_tag="PKW60_08200"
     CDS             295..1050
                     /locus_tag="PKW60_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPJ99450.1"
                     /translation="MILLLWALLSAAEPENVDAFLEELSARRAAVRVVQARLDRENVT
                     TDGVFEAKGTVLFVHPRRIVFRYDESAETDVLIDGLRLYRYDRDMEQLQIDDLEDDPA
                     SEAFFLGFAKDFKRLRKAFDVSLFIPEIKEGEVKGLLLRPKKVESEDESGLASPAAAF
                     EEVYLYLREPDYLPTRVIVVNDANSRVEIRLSDYRVNEAVESAETQFWIPEGTVIIEN
                     QETFEEAPAGGLFLPRDPAVVDIPLSAEETPAP"
     gene            1047..1655
                     /locus_tag="PKW60_08205"
     CDS             1047..1655
                     /locus_tag="PKW60_08205"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPJ99451.1"
                     /translation="MKRLVLASASPRRRALLAALGLDFDVITSGAPEIENGLPPEQLV
                     VANACAKRDEVARRLTEPALVIAADTLVFLDGEVLNKPAGLDEARDMLARLSGRTHAV
                     LTGLALADTETGAVAQGFERTEVTFRELSEEEIAYFVNTVRPIDRAGAYTVDGPGCLL
                     VASYSGCYPNVLGLPLVRLDLLMRELGWNLFEWMDPARANFL"
     gene            1767..3134
                     /locus_tag="PKW60_08210"
     CDS             1767..3134
                     /locus_tag="PKW60_08210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPJ99452.1"
                     /translation="MNTVEFTVLGGGTEIGANCYCVSGAGTGIILDCGLHPKKEGREA
                     LPDLSILTRPPDVALVSHGHVDHCGAIPYLLRQFPGLFVYTTRPTVPIMDRMLHNSVS
                     VMEMLERNQGVRGYPLYGHDDVGFALRRTAGLEMEHEFALTHQSPVRAEFRSAGHVLG
                     SACILLQFPGHTVFYTGDICLTHQELMRGHDLLEEEIQVDTLICESTRGAQNSDSERS
                     YEDEIERFAAESTKVLESGGAVLVPAFALGRNQEILNIIARLKRSRMIPRVPVYSSGL
                     GRAIYEIYSDNLDYLGPRADLRPLDEFQRVGNVWEPETVDELLEEPCIIVATSGMMVE
                     NTPSALIAKEMVRSTRHGIFFVGYLDPDTLGYRLLHANTGDKMPFETPGRSVTIRLGN
                     RQRFHFSAHAPREDLRRLIEHLNPKNLVFVHGDAPAVQWMASNTNGRARKFSPMLGQT
                     ITLEA"
     gene            3138..4088
                     /locus_tag="PKW60_08215"
     CDS             3138..4088
                     /locus_tag="PKW60_08215"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPJ99453.1"
                     /translation="MARRRNPLGVWLLTSLCLGFAWLTRVLPLSWARALGALLAAVAY
                     HVLPRIQRVGMANLDLAYGDSLSAAEKIRILKESVRNVGLVAAEFTRTPLLGDDAFFT
                     RWVRIEGAEHLARDRSAILIGPHIGNWEWLAGAFARLGRPAAEIVRPLHDPRLNRFID
                     RIRSSNAVKTIEKSGAGPEVLRLLREGHCVGLLIDQSPHQSAVPVRFFGVETWGTAAP
                     AILSARAKAPVHVAALIREPDGRYVLRISPRVVLSAEGGQRARMLDNAQRCQDAIEAL
                     IRAYPEQWLWIHRRWKPRPRFEREWTQKQAQRDARTGGDV"
     gene            complement(4166..5218)
                     /locus_tag="PKW60_08220"
     CDS             complement(4166..5218)
                     /locus_tag="PKW60_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99454.1"
                     /translation="MPKDLNFAVLGLGIGMTHCEAIVDAKGARLAAACDHDEARLHAA
                     VTKFGCEGYTKYTDLLKNKDIDAVCIATESGLHATHGIQAARAGKHLLVEKPVDVTPA
                     RIRQLRDAVNRAGVKCGCVFQLRTEPVNLRLKKAIDSGKLGRLIGVHAQLPWFRAQSY
                     YEGPHGAWKGTWKLDGGGSLMNQGIHTVDLVQWLAGPVESVCGFIGVFGHAIEAEDQT
                     VAILRFESGALGTLVTTTCAIPDQSQRILVYGEKGSFTKTVYLESFDAGTPKERRQMM
                     AWYGEKKTSRLGRDPLAVGAAGHVRHVEDLVKAIHRDTQPGITIEDATHAVEIASAIF
                     RSARTGKVVKIKDVRK"
BASE COUNT          968 a         1587 c         1823 g         1096 t
ORIGIN      
        1 gagcctgctc gacagcctga tcccagaaga tgacggcgtg ccgacgagct ttttcgacgc
       61 cggtgtggcg agcgatacgg acctggatga tgacgacaag gatctgccgg tcgtcaatat
      121 cgccgatgac aacactgact cagagggcgg gatgggcgga ttcgcgtcgt tttcgcccgg
      181 tgaaccggcg gatgctcccg ccggcccggc gatggaactc gggccccgcg cagcggattc
      241 acagagaggc tcgcaggaac tgaaatcccc tgcgcctgag gaggatgctg catcgtgatc
      301 ctgctgttgt gggccctgct ctcggcggct gagccggaga atgtggatgc gtttctggag
      361 gagctgtccg cccggcgggc cgcagtccgt gtggtgcagg cgcgcctgga tcgcgaaaac
      421 gtgacgacgg atggcgtgtt tgaagccaaa ggcacggtgc tgtttgtcca tccccggcgt
      481 attgtgttcc ggtacgacga gtcggccgaa accgacgtgc tgatcgacgg gctgcgtttg
      541 taccggtacg acagagacat ggagcagctc cagatcgacg atttggagga cgatccggcc
      601 tcggaagcgt tttttttggg ctttgcgaaa gatttcaaac gtctgcggaa agcgttcgat
      661 gtatcgctgt tcatcccgga gatcaaagaa ggggaggtga aaggcctcct gcttcgcccc
      721 aaaaaagtag aatcggagga cgaatccgga ctcgcgagcc ctgcagcggc ctttgaagag
      781 gtttatttgt acctgcgcga acctgactac ctcccgacgc gggtgatcgt agtcaatgac
      841 gcgaattcgc gcgtggagat tcgcctgtcg gactatcgtg tcaacgaggc ggtggaatcg
      901 gccgaaaccc agttctggat ccccgagggc accgtcatca ttgagaatca ggagaccttc
      961 gaagaagcgc ctgcgggcgg cctgtttctg ccccgagatc cggccgtggt ggatatcccc
     1021 ctctccgcag aggagacccc ggccccgtga aacgcctcgt gcttgcctcg gcttcgccgc
     1081 gccggcgcgc cctgctggcg gctttgggcc tcgactttga cgtgatcacc agcggcgctc
     1141 cggaaatcga aaacggtttg ccgcccgaac agctcgttgt ggccaatgcg tgtgcgaaac
     1201 gcgacgaagt ggcgcgccgt ctgacggagc ccgcgctcgt gattgcggcc gatacgctgg
     1261 tctttctcga cggcgaggta ctcaataagc cggccggcct cgacgaggcg cgcgatatgc
     1321 tggcgcggct ttccggccgc acacatgcgg tgctcacggg ccttgcgctg gccgataccg
     1381 aaaccggtgc cgtggcgcag ggtttcgagc gcacggaagt cacattccgg gagttgtcag
     1441 aagaagaaat cgcttatttt gtgaacacgg tacgccccat tgaccgggcg ggagcctata
     1501 cggtcgatgg cccgggatgc cttctggtgg cgtcgtattc gggatgttat cccaatgtgc
     1561 tgggcttgcc gcttgtccgc ctggatttgc tgatgcggga attgggctgg aatctgttcg
     1621 aatggatgga cccagcgcgg gcgaatttcc tgtaagtgcc cccgccttcg cgcgctgccc
     1681 cggccgtccc cggcggagga acccggccgg gcgagttgac ctcgaccgcg ccggggggta
     1741 cactttcctt ttgtgaggat tgctacgtga acactgttga attcaccgtt ctgggcggtg
     1801 gcacagaaat tggcgcaaac tgttactgcg tgagtggagc cggcacgggc atcattctgg
     1861 actgtggcct tcatcccaaa aaagagggcc gtgaagcgct tcctgatctg tccatcctga
     1921 cgcggccgcc tgatgtcgcg ctggtctccc acgggcacgt ggatcactgc ggggcgattc
     1981 cctacctgct ccgccagttt ccggggttgt ttgtatacac gacgcgtccg acggtgccca
     2041 tcatggatcg catgctgcac aacagcgtat cggtcatgga gatgctggag cggaaccagg
     2101 gcgtgcgggg atatcccctc tatggccacg acgacgtcgg ctttgccctt cggcggacgg
     2161 ctgggctgga gatggaacat gagttcgccc tgacccacca gagccccgtc cgggcggaat
     2221 tccgctctgc gggccatgtg ctggggagcg cgtgcattct attgcagttt ccggggcata
     2281 cggtcttcta cacgggcgat atttgtctga cgcaccagga attgatgcgc ggccacgacc
     2341 tgcttgagga agaaatccag gtcgacacgc tgatctgtga aagtacgcgc ggcgcccaga
     2401 attcagacag cgaacgaagc tatgaagacg agatcgagcg atttgccgcc gaaagcacca
     2461 aggtgctgga gtctggcgga gcagtgctcg tgcccgcatt tgcgctgggc cgcaaccagg
     2521 agatcctcaa catcatcgcg cgcttgaagc ggtcgcggat gattccccgc gtgcccgtat
     2581 attcatcggg gctggggcgg gcgatctatg agatctacag cgacaacctg gattatctgg
     2641 gcccgcgcgc cgatctgcgg ccacttgatg aattccagcg cgtgggcaac gtctgggaac
     2701 ccgagaccgt cgacgagctg cttgaggaac catgcatcat cgtggcgacg tccggcatga
     2761 tggtggagaa tacgccgtcg gccttgatcg cgaaggagat ggtgcgctcg acgcggcacg
     2821 gcattttctt cgtgggctac cttgatccgg atacgctggg gtaccgcctg ttgcacgcga
     2881 atacgggcga caagatgccg ttcgagacgc cgggacgctc ggtgaccatc cggctgggca
     2941 accgccagcg ctttcatttc agcgcgcatg cgccgcggga agatctccgg cgcctgatcg
     3001 agcacctcaa cccgaagaat cttgtgtttg tccacggcga tgcgccggcg gtgcaatgga
     3061 tggcctcgaa taccaacggc cgggcgcgta aattctcgcc catgctcggg cagaccatca
     3121 ccctggaagc ctaggccgtg gcgcgccgcc gcaatccgct gggtgtatgg ctcctgactt
     3181 cgctatgcct gggatttgcc tggttgaccc gggtgctgcc gctttcctgg gcgcgcgcgc
     3241 tgggcgcgct gctggcggcc gtggcctatc acgtgctccc tcgcatccag cgggtgggga
     3301 tggccaatct tgacctggcg tacggcgatt ccctgtccgc agcggaaaag atccggatcc
     3361 tgaaagaatc ggtgagaaat gtcggcctgg tcgcggcgga gttcacccgc acgccgctgt
     3421 tgggcgatga tgcgttcttt acccggtggg tgcgcatcga gggcgcggaa cacctcgcgc
     3481 gtgaccgtag cgccatcctg atcggcccgc atattggaaa ctgggaatgg ctcgcgggag
     3541 cctttgcgcg tcttgggcgg cccgcggcgg agatcgtgcg gccactgcac gatccgcgct
     3601 tgaacagatt cattgaccgg attcgcagtt ccaacgccgt caagacaatc gagaaatcgg
     3661 gggccggtcc cgaagtgctg cggctgcttc gggaaggcca ctgcgtgggc cttctcattg
     3721 atcaaagccc tcatcaaagc gcggtgccgg tgcgcttctt cggggtggaa acctggggca
     3781 cggcggctcc ggcgatcttg tctgcgcgcg cgaaggcgcc ggttcatgtg gccgcgttga
     3841 ttcgtgagcc ggacggccgg tacgtgctgc gcatctcccc ccgggtggtt ctgagcgcgg
     3901 aaggcggcca gcgcgcgcga atgctcgaca acgcgcaacg ctgccaggat gccattgagg
     3961 cgctgatccg cgcatacccc gaacagtggc tgtggattca ccggcgttgg aagccgcgtc
     4021 cgcggttcga gcgggaatgg acccaaaaac aggcgcagcg ggatgcccgg accggtgggg
     4081 atgtgtgagc gcgccgaatt ggtgattcgc acgaaggggc gccccatgcg caggccgcaa
     4141 acgggaaatc cggctttttc aggagctatt tccgcacgtc tttgatcttc acgactttac
     4201 cggttcgggc ggatctgaag atggcagagg cgatttcgac cgcgtgggtg gcgtcctcga
     4261 tggtgatgcc gggctgggta tctcgatgga tggcttttac gaggtcttcg acatgccgga
     4321 catggccggc cgcgccgaca gcgagaggat cccggccgag gcggctggtc tttttctccc
     4381 cgtaccatgc catcatctgg cgccgctctt tcggggtgcc ggcgtcgaac gattcgaggt
     4441 agaccgtctt ggtgaaggat cccttctcac catagacgag aatgcgctgg ctctgatcgg
     4501 gaatggcgca ggtggtggtg acgagggtgc cgagggcgcc ggattcgaag cggagaatgg
     4561 ccactgtctg atcctccgcc tcgatcgcat ggccgaacac gccgatgaat ccgcacacgc
     4621 tttcgaccgg tcccgccagc cactggacga gatcaacggt gtggatgccc tggttcatga
     4681 ggctacctcc accgtcgagt ttccaggtgc ctttccaggc tccatgaggg ccttcgtaat
     4741 aactctgcgc gcgaaaccac ggcagctggg cgtgaacgcc gatcagccgg ccgagtttcc
     4801 cgctgtcgat ggccttcttc aggcggaggt ttacgggctc ggttcgcagc tggaacacac
     4861 agccgcattt gacgccggcc cggttaacgg catcgcgcag ctgcctgatg cgggcaggag
     4921 tgacgtcgac gggcttctcc acgaggaggt gctttcctgc gcgggcggct tgaatgccgt
     4981 gggtggcgtg gagcccgctt tcggttgcga tgcaaacggc atcgatgtcc ttgttcttga
     5041 gcagatcggt gtattttgta tagccctcac agccgaattt tgtgacggca gcgtgaaggc
     5101 gggcttcgtc atggtcgcag gcggcggcaa ggcgggcgcc tttcgcatcg acgatggcct
     5161 cgcagtgtgt catccctatt ccgagaccga gcaccgcaaa attcaggtct tttggcatgg
     5221 ttgatcctct ctgtgatgag tggcaggttc acgaacgggc gggcgaaccc ggagtgcggt
     5281 tgcatgccgg cgacagaata ggaaaagcag tgacgggcat tcaagcgtat gcttcaccat
     5341 attgcgacgt gctgcttgaa cctcggggaa gcggcctgtc aggatggggc gcgcatctga
     5401 aagcggatta ggctcatgtt gcagggagaa caggcatgag aggctggttc cgtcaacacc
     5461 gcttcgcgct cggt
//
LOCUS       DAOPBQ010000220         5450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12824_length_5450_cov_3.897868, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000220 DAOPBQ010000000
VERSION     DAOPBQ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12824_length_5450_cov_3.897868"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PKW60_08225"
     CDS             complement(<1..449)
                     /locus_tag="PKW60_08225"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPJ99455.1"
                     /translation="MSNTRRDFLKTAGGVVAAGAAIHGGLAVSAQAAAPAGGRTEHLL
                     PPLPYAYDALEPVISAEIMQLHHDKHHAGYVKGLNAAEAALAEARAAGDYALVQHWSR
                     QAAFNGGGHYLHSMFWKVMAPPDKGGGGEPSGALAAKITEDFGSFAN"
     gene            676..1236
                     /gene="rfbC"
                     /locus_tag="PKW60_08230"
     CDS             676..1236
                     /gene="rfbC"
                     /locus_tag="PKW60_08230"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPJ99456.1"
                     /translation="MRVTIENTRLPGVLLVKAGIARDARGFFSEIYSQQTFAEAGLDA
                     QFVQDNLSLSAKGIFRGLHYQLEPFAMGKLVRAIRGAVFDVVVDLRRDSPTFGQWLGV
                     TLTEENHLALWAPPGFAHGFLTLEDQTLFHYKCTQIHTPEAERSIRYDDPEIGIELPM
                     PPSLLSEKDAKAPLLADAEYNFQYSG"
     gene            complement(2006..3244)
                     /locus_tag="PKW60_08235"
     CDS             complement(2006..3244)
                     /locus_tag="PKW60_08235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPJ99457.1"
                     /translation="MKRWFWLLAATTPIAFIASVTWYGAFTFANAYMIRGLGASNAQW
                     TRATLAMSGGMLVWYLFTVEMSALLGRRQTATLGTSIAALAYISLPFAHEVWMVSVLL
                     ALMGFGTAGYLVAWLPYVTHVGGNRPGRALTMASISYNLISGIAIFALGPALQAAHFH
                     FVFFLTGGACLGCLVLFYFAARHLEAQADTSAAEENQPARPTDPLSLLKLQWHDVREL
                     VRSAFPIVLLLGICAAPFAFHTSNQLYPNLMRDIHHLQEDAIARLVAVGRVPAMLTLF
                     LMSPIIDRLNLVRFYGLFLFTDGLLIAAIALAQSTLMAGTAYLVFYLSHGFVWAFALP
                     AINVCVRPRLRDSAFALTAMFEILGVFFAGMLHNRLLEAGCSLPCVFVVCGVVAGLSG
                     LLLLCYSFSGHSRRNGRPAI"
     gene            complement(3263..>5450)
                     /locus_tag="PKW60_08240"
     CDS             complement(3263..>5450)
                     /locus_tag="PKW60_08240"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPJ99458.1"
                     /translation="ASVALLTPHHVIAAETPNPPEYYTIPVIDLSHDQERQVAVDREP
                     GQYLGHPTTVLLEDNQTMICVYPKGHGRGAVVMKRSHDAGKTWSDRLPVPESWTTSQE
                     VPTLYRVEDPAGVKRLIVFSGLYPIRMACSDDDGLTWSELEPIGDFGGIVAMASLMRL
                     KNGDYLAFFHDDGRFLKGENKNGPFVVYSTRSTDGGLTWGDPEIVVQHAEAHLCEPGA
                     LRSPDGAQIALLLRENSRKFNSFVTYSDDEGVTWSEPVQLPASLTGDRHTARYAPDGR
                     LVVVFRDTTRVSPTYGDFAGWIGTYDDIVLGREGQYRLRLLDNTKGADTSYPGLELLP
                     DGTFVTTTYGHWAEGEEPYIMSVRFTLDEIDAKAAQLPLEKPVFVSGEGGYHTYRIPS
                     LIATPAGTLLAFCEGRKESTSDAGDIDLLLKRSTDNGATWNSQQVVWDDGPNTCGNPC
                     PVVDHSTGVIWLLLTHNLGRDSEKAIVEGQSTGTRTVWVSHSSDDGLTWAAPKEITAT
                     TKKPEWTWYATGPGVGIQLRRGSHPGRLVIPCDHIVAESKTYHSHVIYSDDHGATWLL
                     GGVTDDTVNECQVIERTDGSLLLNSRRWHTNTGNGRAIATSPDAGMTWSDLANDPALI
                     EPRCQASLIRYRPSDAEELPVVLFSNPADAERRYKMVVRLSVDDGRTWPAWKCLYPGP
                     AAYSCLTVLSDGRVGCLYERGTKHPYEMIVFSAFPLNWAGEGVLDR"
BASE COUNT         1150 a         1565 c         1641 g         1094 t
ORIGIN      
        1 aaattcgcaa agctgccgaa atcctcggtg attttagccg caagcgcgcc gctgggctct
       61 ccgccgcctc ctttgtccgg cggggccatt accttccaga acatcgagtg aaggtagtgc
      121 ccgccgccat tgaaggcagc ctgtcgtgac cagtgctgca ccagtgcgta atcgcccgcg
      181 gcgcgggctt ccgcgagcgc cgcttccgcg gcattcaatc cttttacata ccccgcatga
      241 tgcttatcgt ggtggagctg catgatctct gcgctgatca cgggctccag cgcgtcatag
      301 gcgtagggca ggggagggag caggtgttcg gtgcgcccgc ccgctggtgc agccgcctga
      361 gccgataccg cgaggccgcc atgaattgcc gcgcccgctg caaccacgcc gcccgcagtc
      421 ttcagaaaat cacgtcttgt atttgacatc gttcggactc ctcattgatc cgtaaggaat
      481 tgccgccttc ccagtcatca gtacgcaaac aacacatccc gcgagaatat tcgcgcctcg
      541 tggctttgca ggcggtcagg tgcggacctc cgccgggttt tgctaccatg cgttcgctgc
      601 ccctgcttcg cgaaggcgcg aagcgcgagt gttgcgcgac atacactcag gtcatggagg
      661 atttccggaa tgccaatgcg ggtcacgatt gagaacaccc ggctccccgg ggtattgctc
      721 gtgaaagcgg gaattgcgcg ggatgcccgg ggcttctttt ccgagattta ttctcagcag
      781 actttcgcgg aggcggggct cgatgcccag tttgtgcagg ataatctcag tttgtctgct
      841 aagggcattt ttcggggtct gcactatcag ctcgagccct tcgccatggg caaactggtc
      901 cgcgcgatac gcggagcagt gttcgacgtg gtcgtagatt tgcggcggga ttcgcccacc
      961 ttcggccagt ggctgggggt gaccctcacg gaagaaaacc acctggccct ctgggcgccc
     1021 cccggctttg cccatggatt cctcacgctt gaagatcaga ccctgttcca ctacaagtgc
     1081 acgcagattc atactccgga ggcagaacgc tccatccgct acgatgaccc cgaaatcggc
     1141 attgaacttc ccatgccgcc ctcactgctc tcggaaaaag acgcaaaagc accccttctg
     1201 gcggatgccg agtacaattt ccagtattcc ggctgagaga ggagaatgca ggcccagccc
     1261 ttggggaagc gggtattgcg cttgagaatg ggcaaaaaaa aggccctggc ggggagaacc
     1321 agggccacgc tcccaaggag gaagcggaat ggagggctac agatgctcaa tgagagtcat
     1381 acatatagcg gaaaatggct aagttaaact gtcttctcga attcgtttcc ggagaacgtt
     1441 gaggcaaccc gttctcgcca gttccgccag ttataggcgc aaagttcgtg ccacgtattc
     1501 acgtgacctg aaccgctgtt cttgaacacc cgggcggtcc gtctgccaaa acctttgtgt
     1561 ttatttgtaa atatcggaaa atacaaactt tatgcgttcc ataacaccta tttcgaggca
     1621 aagaatgcct ttttgagcag aaatcggtct tttctgggga taattgatgt aaatcaagcg
     1681 aaatcaatga tttaatttga ccgtgctccg gaagaagcag ggactctgtt cttctttctc
     1741 actggacctt cgttcgcatt ccacataccc gcactcgatg aattccggca actcacgatc
     1801 agatttactc aaaactgaat aacatgcgcg cattttcaag gatcgctgat cgaatatttc
     1861 agtttgaagc gggattttcg agacaagtcg accccttatc acgggcatat ctagaattca
     1921 aggaaatgaa ggcgacgaag gaagcggcca ttctgaagtt gtaaagaatt tatttccagt
     1981 cacttacaaa gccagagccg catcttcaga tggctggccg gccgtttctc cgggagtggc
     2041 cggaaaacga gtagcagagc agcagcagtc ctgaaagacc tgccacgaca ccacagacca
     2101 caaaaacgca ggggaggctg caaccggcct cgaggaggcg gttatgcaac atgcctgcaa
     2161 agaaaacgcc gaggatctcg aacatcgcgg tcagcgcgaa tgccgagtcg cggagccgcg
     2221 gccggacaca cacgttgatg gcaggcaatg caaatgccca gacgaaacca tgggacagat
     2281 agaagaccag gtaggctgtg cccgccatca gtgtgctctg cgccagcgcg atggcggcga
     2341 tgagaagccc atcggtaaaa aggaaaagcc cgtaaaatcg tacgaggttc agccggtcga
     2401 tgatgggaga catgagaaac agggtcagca tggcggggac gcgtcccacg gccaccagac
     2461 gtgcaatagc gtcctcctga agatgatgaa tgtcgcgcat gagattgggg tacagctggt
     2521 tggaggtatg aaacgcgaac ggagcggcgc aaatccccag cagcagcacg atggggaagg
     2581 cgctgcgcac cagttcacgc acgtcgtgcc actgcagctt caagaggctc aggggatccg
     2641 tcgggcgtgc cggctggttt tcttcggcag cacttgtatc ggcctgggct tcgagatgcc
     2701 gggcggcaaa ataaaacaga accaggcagc ccaggcaagc gcctcccgtc aagaagaata
     2761 caaaatggaa atgcgccgcc tggagggcgg gcccgagggc aaagatagcg atcccggaga
     2821 tcagattgta tgagatggat gccatcgtca gggcgcggcc cggccgattg ccaccgacgt
     2881 gggtgacata aggcagccag gcgaccaggt agcccgccgt cccaaatccc atgagggcga
     2941 gcaacacaga caccatccaa acctcgtgcg cgaaaggcag ggagatgtat gcgagggcgg
     3001 ctatcgacgt gcccagggtg gcggtctgtc ggcggcccag cagggcggac atctccaccg
     3061 taaacaggta ccagaccagc atgccgccgg acatggcaag ggtggcgcgg gtccattggg
     3121 cattcgaggc gcccagcccg cgtatcatgt aggcattcgc aaacgtgaac gccccatacc
     3181 aggttacgga tgcaatgaag gcaataggtg tagtggctgc caggagccag aaccagcgtt
     3241 tcatacaggc aggctttcca ggttaccggt ctaaaactcc ctcaccggcc cagttcagcg
     3301 ggaatgctga gaatacaatc atttcatacg gatgtttcgt gccccgttca tacaggcaac
     3361 ccacgcggcc gtccgatagc accgtgaggc acgagtacgc tgcggggccc gggtagaggc
     3421 atttccaggc gggccacgtg cggccgtcgt ccacactcag gcggaccacc atcttgtatc
     3481 gtctctcggc gtctgccgga ttcgaaaaca agacgacggg tagctcttct gcgtcgctcg
     3541 gccggtagcg gatcaggctt gcctggcagc gcggctcgat cagcgccgga tcgttcgcca
     3601 agtccgacca ggtcatgccc gcgtcaggac tggtggcgat ggcacggccg tttcctgtgt
     3661 tggtatgcca gcggcggctg ttgagcagga ggctgccgtc tgtgcgctcg ataacctggc
     3721 actcgttcac ggtatcatcc gtcactccgc ccagcagcca tgtagcaccg tggtcgtcgg
     3781 aatagatcac gtgtgagtga taggtcttgc tttccgcgac gatatggtcg caagggataa
     3841 cgaggcggcc cgggtggctg ccccgccgca attgtatccc aacgcccggg cccgtggcat
     3901 accaggtcca ttcgggcttc ttcgtggtcg cggtgatttc tttgggagcc gcccaggtca
     3961 gaccgtcgtc gctgctgtga gaaacccaga ccgtgcgtgt gccggtactc tgaccttcga
     4021 caatggcctt ctcgctgtct cgtccgagat tatgggtcag gaggagccag atcacgccag
     4081 tggagtgatc gacgacgggg caggggttgc cgcaggtatt gggtccgtcg tcccacacga
     4141 cctgttgtga gttccaggtg gcgccgttgt ctgtgcttcg tttaagcaga aggtcgatgt
     4201 cgcccgcgtc gctggtgctc tccttgcgtc cttcgcagaa ggcgagcagc gtgcccgcag
     4261 gtgtggcgat gagcgagggg atacggtagg tgtgataacc gccttctccg ctcacaaaaa
     4321 caggcttttc caggggcaat tgcgccgctt tggcgtcgat ttcatccagc gtgaaacgga
     4381 cgctcatgat gtacggctct tctccctcgg cccaatggcc gtacgtggtc gtgacaaacg
     4441 tgccgtcagg gagcaactcg agcccaggat aggaagtgtc cgcccctttg gtgttatcga
     4501 gcagccgcag gcggtattgc ccttcgcggc cgagcacgat gtcgtcgtat gtgccgatcc
     4561 agcccgcgaa atccccgtag gtcgggctga cgcgcgtcgt gtcgcgaaag acgacgacca
     4621 aacggccatc cggcgcgtac cgggctgtgt ggcggtcgcc cgtcaggctg gcggggagct
     4681 gcacgggctc agaccacgtg actccttcgt cgtcgctgta ggtgacaaac gaattgaatt
     4741 ttcggctgtt ttcacgcagc agcaaggcga tttgagcgcc atctggcgag cggagggcgc
     4801 caggctcaca caaatgcgcc tcggcatgct gcaccacgat ttccgggtcc ccccaagtca
     4861 agcccccatc ggtcgaccgg gtggaataga cgacgaaggg gccgtttttg ttttcacctt
     4921 tgagaaatcg tccatcgtca tgaaaaaatg ccagataatc gccgtttttg agccgcatga
     4981 gcgacgccat cgccacaatg ccgccgaaat caccgatggg ttccagttct gaccaggtca
     5041 gaccgtcgtc gtccgagcat gccatgcgga tgggatacag acccgaaaac acgatcaggc
     5101 gtttgacgcc ggccggatcc tccacgcggt agagcgtggg gacttcctgc gacgttgtcc
     5161 aactctccgg aactggaagc ctgtcgctcc aggttttccc cgcgtcgtgg ctgcgtttca
     5221 tgacgaccgc gcctcggccg tgtcccttcg ggtacacaca aatcatggtc tggttgtctt
     5281 cgagcaggac ggtggtggga tggcccaggt actgtcccgg ctcgcgatcc actgcaacct
     5341 ggcgctcctg gtcgtgcgac agatcgatca cggggattgt gtagtactcg ggtgggttgg
     5401 gtgtttccgc cgcaatcacg tgatgcggtg tcagcagtgc aaccgatgcc
//
LOCUS       DAOPBQ010000221         5404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_12956_length_5404_cov_4.257244, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000221 DAOPBQ010000000
VERSION     DAOPBQ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12956_length_5404_cov_4.257244"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /gene="glgA"
                     /locus_tag="PKW60_08245"
     CDS             <1..1432
                     /gene="glgA"
                     /locus_tag="PKW60_08245"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HPJ99459.1"
                     /translation="ASEIAPFAKTGGLADVANSLPKALTALGHDVRLVMPCYASIPKD
                     LLGKRLGAFMVYLGGNHRSGAVRLSSLPGSRVPVYLIEQGLYFARSGLYQTGGKPYSD
                     NLERFAFFALSALEGVAQTGWLPDVIHSNDWHTALAPAFLKVSQRVNPAWRGIPTVFT
                     IHNLIYQGRFPGMQFYQTGLPERLFTPEFLEYYGDVNVMKAGIAFASRVSTVSPRYAE
                     EIQTLEGGCGLDGFLRTRSADISGILNGIDYSVWNPEDDPHIPAHFSRKNLKGKTRCK
                     SALQAELGLPEKNIPLFGCVSRLVYEKGIDLVIDALEGLLRQELQLAVLGTGDPELER
                     RLAEASQRHPERLKVILRYDEALAHRFYAGCDFFLMPSRSEPCGLSQMYSLAYGAIPI
                     VRNTGGLANSVQDATPENMSGGKATGIVFQHPAVEDFTQGVRRALELYQDCDKRRRLR
                     KAGMAEDFSWERSSREYDKLYREALA"
     gene            1429..1914
                     /gene="tsaE"
                     /locus_tag="PKW60_08250"
     CDS             1429..1914
                     /gene="tsaE"
                     /locus_tag="PKW60_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPJ99460.1"
                     /translation="MSRLVQDCVIESASPEATRRLGAALAALLPRGAVVALRGDLATG
                     KTCLVSGMATHFTEADYVHSPTFTLVNQYGEEPALVHVDLYRLGGPDEVAELGYAELF
                     DSDDVCVVEWAERAENLLPEVRMDIWLEHAGYDLRRLTFHNRDLLDGDWQSRLRSAAG
                     L"
     gene            complement(1935..4223)
                     /locus_tag="PKW60_08255"
     CDS             complement(1935..4223)
                     /locus_tag="PKW60_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPJ99461.1"
                     /translation="MRVIQWLWNLAGWPLEYAGKLMGREFAAAWQLIAKLFVVLIVLY
                     FLVDICSRILKIVQRGRRSRWVDAADIDALASMKNLDVVDRVRSRQQLKEQARQLKAD
                     KEYGKLANLYSEAGYPEEAARWYRKIGNHHKAAMEVAKAGDTLKAARLFLREGDYSSA
                     AQYFMENARFEEAASAYEKAGKPVLAASAYGRAGNTAQALQMFAHYFRSTGDAMEIQI
                     QAANECYSALQNPQLSESISEEQRRELFPLLAERFERMNKHELAAQLFKNGGDLARAG
                     ETYLLAGKLEEAAACMEQAGKKQEAMRIVGRAHEAKGNWREAGTAYAEAGEFQRAAAA
                     FSKAKDAGRAADCYARAGDFYHAGLAFAHAGRFSDAIKILQRVKEEHPSFELSRGLLG
                     RCFYELHDYAHCVAALENHLTGKRVDNALLDYFYMLALAYEQTGRLDESLQILRKIHT
                     VDVDFRDVTNRISSISTRLSMAVGPNTVLQGGVPGATSGQMMKSVENALEGRYDLQKE
                     LGRGGMGVVYLAKDRQLDRPVAIKFLGSLIDDSKEFRERFVREARTAAKISHPNIVSI
                     YDISASEGRAYIAMEYVEGMSLYRFLARKGAIPPREAVNYLVQACSALAALHDAGITH
                     RDIKPDNILIAKGGLVKIMDFGLAKSEDNRLTRTGVVMGTPSYMSPEQARGKEADARS
                     DIYSIGMVLHECLTGQIVFASGNVVMRQMQEMPPPPSATNNAVSEELDGIVMKCIQKK
                     PEDRYQSMHELAAALRALDLNS"
     gene            4525..5250
                     /locus_tag="PKW60_08260"
     CDS             4525..5250
                     /locus_tag="PKW60_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPJ99462.1"
                     /translation="MRYGILSDIHANLEAMEAVLEKLDELDVDQVLCLGDVVGYNASP
                     NECADLVRQWDLPTVCGNHDAVACGREEPWGFNIVALEAAMWTRDHLTPENIHWLEAL
                     PDAVQPDGLIGVHGAPGDRDTYLFTWGDVSPHFDDSHVKGRNACFFGHTHVPGIFEAD
                     GGWTPVNGTTFALKNGRVTFINPGSVGQPRDGDPRASFGVYDTVSREFSLMRVRYDVQ
                     SAMDRIKRAGLPDYLAHRLEFGR"
     gene            5274..>5404
                     /gene="hisB"
                     /locus_tag="PKW60_08265"
     CDS             5274..>5404
                     /gene="hisB"
                     /locus_tag="PKW60_08265"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657484.1"
                     /note="catalyzes the dehydration of
                     D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate to
                     3-(imidazol-4-yl)-2-oxopropyl phosphate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HPJ99463.1"
                     /translation="MREASVQRETKETRIALKLQLDGAGAADIQTGVGFFDHMLTHIA
                     "
BASE COUNT          999 a         1573 c         1663 g         1169 t
ORIGIN      
        1 ggcatcggaa attgcccctt ttgcaaagac gggcggttta gccgatgtcg cgaattcgtt
       61 gcctaaggcg ctcacggccc tggggcatga tgtgcgcctg gtcatgccct gctatgccag
      121 catcccgaaa gatttgctcg gaaagcgctt gggcgcattc atggtgtatc tgggcggaaa
      181 ccaccggtcg ggggccgtgc gtctgtcgtc gctgccggga tcccgggtgc cggtgtattt
      241 gatcgaacaa ggcctgtatt ttgcgcgcag cggtttgtat caaaccgggg gcaaacccta
      301 ctcggacaac ctggagcgat ttgccttctt cgccctgagc gcactggaag gcgtcgcaca
      361 aaccggctgg ctgccggatg tgattcacag caacgactgg cacactgcgc tggccccggc
      421 gttccttaaa gtatcacagc gtgttaatcc cgcctggcgc ggcatcccga ccgtgttcac
      481 cattcacaac ctgatctatc agggccgctt tccaggcatg cagttctatc agacggggct
      541 cccggagcgg cttttcacgc ccgagttcct cgaatactac ggcgacgtga acgtcatgaa
      601 agcaggcatc gccttcgcga gccgagtcag caccgtgagt ccacggtacg cggaagaaat
      661 tcagaccctg gagggcggat gcggtttgga cggttttctg cgcacgcgtt cggcggacat
      721 cagcggcatt ttgaacggga ttgattacag cgtgtggaat cccgaggatg atccgcatat
      781 ccccgcgcac ttttcccgga aaaacctcaa ggggaaaacc cgctgtaaat ctgcgcttca
      841 ggccgaactg ggcctgccgg aaaaaaacat tccgctgttc ggatgcgtgt cgcgcctggt
      901 ctatgaaaag ggtatcgacc tcgtgatcga tgcactggag gggctccttc gccaagagct
      961 acagctcgcc gtcctcggca cgggagatcc cgagctggag cgccgcctgg cagaggcttc
     1021 tcagcggcat cccgaacgct tgaaagtcat cctgcgctac gatgaagcgc tggcccatcg
     1081 attctatgca gggtgcgatt ttttcctcat gccttcgcgc tccgaaccgt gcggtttgtc
     1141 gcagatgtac agtctggcct atggcgccat tccgatcgtt cgcaacaccg gaggtctcgc
     1201 caattcggtc caggatgcca cccccgaaaa catgtccgga ggcaaggcca cgggaattgt
     1261 cttccagcac cctgccgtgg aagattttac gcagggcgtc cggcgggcgt tggagcttta
     1321 ccaggactgc gacaaacggc gccgcctgcg aaaggccggc atggccgagg atttttcgtg
     1381 ggaacgctcc tcgcgcgaat acgataagct ctaccgcgag gctctggcat gagcaggctc
     1441 gttcaggact gcgttatcga gagtgcctca ccggaagcga cccggcgcct gggggcggct
     1501 ctggcggcgc tgctcccgcg aggcgcggtg gtggcgctgc gaggcgacct cgcaacgggc
     1561 aagacctgcc tggtctcggg aatggccact catttcacgg aggccgatta cgtacacagt
     1621 cctactttta ccctcgtgaa ccaatacgga gaagagccgg ctctggtgca tgttgatcta
     1681 taccgcctgg gcgggcccga tgaagtggct gagctgggct acgcagagct gttcgattca
     1741 gatgatgtct gtgtcgtgga gtgggcggaa cgcgcggaga atctgctgcc ggaagtccgg
     1801 atggatatct ggctcgaaca cgcggggtac gacttgcgcc gcctgacttt tcacaaccgc
     1861 gacctgcttg acggcgactg gcagagccgg ctccggagtg cagccggttt gtgagcccgg
     1921 tgggacgggg cgggtcagct gttcaaatcc agggcgcgaa gagcagccgc cagctcgtgc
     1981 atgctttgat accggtcctc gggcttcttc tggatgcact tcatgacgat gccgtcgagt
     2041 tcttcgctga cggcgttgtt ggtggcgctg ggcggaggag gcatttcctg catctgccgc
     2101 ataaccacat tcccacttgc aaacacgatc tggcccgtga ggcactcgtg gagcaccatt
     2161 ccaatggaat aaatatcgct tcgcgcatcc gcttctttgc cgcgggcctg ttccggcgac
     2221 atgtagctgg gggtgcccat gaccaccccg gtccgggtca ggcggttatc ctcagacttc
     2281 gctagtccga aatccatgat tttgacgagg ccccctttcg cgatcaggat attgtccggt
     2341 tttatgtcgc ggtgggtaat tccggcgtca tgaagggccg cgagtgctga acaggcttgc
     2401 acgagatagt tgaccgcctc ccgcggggga atcgcgccct ttctggccag aaatcgatac
     2461 aggctcatcc cttccacgta ttccatggca atgtacgccc ggccctcact ggcgctgata
     2521 tcgtagatgc tcacgatatt ggggtgggag attttggccg cggtccgggc ttcccgcaca
     2581 aaccgctctc gaaactcctt ggaatcgtcg atgagtgagc ccagaaactt gatggccacc
     2641 ggacggtcaa gctgtctgtc tttggccagg tagacgaccc ccattccgcc gcggcccagc
     2701 tccttctgga ggtcatagcg tccttcgagg gcgttctcca ccgatttcat catttgacca
     2761 ctggttgcgc ccggcacgcc accctgcagc acggtgttgg ggccgactgc catggaaagc
     2821 cgtgtggaaa tgctggagat ccggttggtg acatcgcgaa aatcgacgtc caccgtgtgg
     2881 atcttgcgca agatctgcag ggattcgtcc agccgccctg tctgttcgta ggccagcgcg
     2941 agcatgtaga agtaatcgag aagcgcgttg tctacccgct ttcctgtaag gtggttttcg
     3001 agcgcggcga cgcagtgggc gtagtcgtgc agttcataaa aacagcgccc cagcaggcct
     3061 cgggaaagct cgaaggatgg gtgctcttct ttcacacgct gcaggatttt gatggcgtcg
     3121 gaaaaacggc cggcatgggc aaaggccagc ccggcatggt agaaatcacc ggcgcgggca
     3181 taacagtcgg cggcacgccc ggcatctttc gccttggaaa aggccgctgc agcgcgttga
     3241 aattcgcccg cctcggcgta ggctgtcccg gcttcacgcc agttgccttt cgcttcatgt
     3301 gcgcggccga cgatgcgcat cgcctcctgc tttttccccg cctgctccat gcaggccgca
     3361 gcttcttcga gtttccccgc cagaaggtag gtttcgcctg cccgcgcgag gtcgccgccg
     3421 ttcttgaaaa gctgcgcggc aagctcgtgt ttgttcatgc gctcgaaacg ctccgcaagc
     3481 aagggaaaga gctcgcgccg ctgctcctca ctgatgcttt cgctgagctg agggttctgg
     3541 agtgcgctgt agcactcatt ggcagcctga atctgaatct ccatggcgtc gccggtcgat
     3601 ctgaagtagt gggcgaacat ctggagcgcc tgggctgtgt ttccggccct gccgtacgct
     3661 gacgcggcca gcacgggctt gcccgccttc tcgtacgccg acgcggcttc ttcgaatcgc
     3721 gcgttctcca tgaagtactg ggctgcggac gagtaatcgc cttctctcag gaaaagccgg
     3781 gccgctttga gggtatcacc tgccttcgcg acctccatgg cggccttgtg gtggtttccg
     3841 attttgcggt accagcgggc ggcttcctcc gggtagcccg cttcggaata caggttggcc
     3901 agtttgccgt actctttgtc tgctttcagt tgcctggcct gttctttcag ttgctggcgg
     3961 gaacgcacgc ggtccaccac atcgaggttt ttcatggagg ccagggcgtc gatgtccgcg
     4021 gcgtccaccc accggctgcg gcgtccgcgc tggacaattt tgagtatgcg ggagcatata
     4081 tctaccagga agtacaagac aatgaggaca acgaacagtt tcgcgatgag ttgccaggcc
     4141 gccgcgaatt ctctccccat gagctttcct gcgtattcca ggggccagcc cgcaaggttc
     4201 cacagccatt ggataacacg catgcacagt gtctccctgc attgttttgc ttcgaccttg
     4261 aatacatcat aaacatcgca cagggcattc cacaaacccc acctggctcc cctggcgccg
     4321 gcattgcgtc acgttccggc atatgagaca atcggtgtga ttgtttgtgg atgaacgcac
     4381 ggtctgatat tgtggtcagg aggccgccat cggtgggaaa ggcggccggg tacatcgggg
     4441 gcgttgcagt acgggttgga ggtattgcgg agaccaggtt ttctggccgc gcgcgggcag
     4501 cagggtgatt caggagattc ggagttgcga tatggaattc tctccgacat ccacgccaat
     4561 ctcgaagcga tggaagcggt gttggagaaa ctggacgagc tggacgtaga tcaggtcctg
     4621 tgcctcggcg atgtcgtggg ctataacgcc agtcccaatg aatgcgccga cctggtgcgg
     4681 caatgggacc ttcccaccgt gtgtggcaat cacgatgcgg tggcatgcgg gcgggaagag
     4741 ccctggggtt tcaacatcgt cgctctggaa gcggccatgt ggacccgcga ccatctgaca
     4801 ccggaaaaca tccactggct ggaagcgctt ccggatgcgg tccaacccga cggccttatc
     4861 ggagtgcatg gcgccccggg cgatcgcgat acctacctgt ttacgtgggg cgatgtcagc
     4921 cctcattttg atgattccca cgtcaaaggc agaaacgcct gtttcttcgg acatacgcat
     4981 gtcccgggta tttttgaggc ggatggcgga tggacgccgg tcaatggcac aacgtttgcc
     5041 ctgaaaaatg gccgagtcac tttcatcaac ccgggctcgg tcggtcagcc ccgggacggc
     5101 gatccccggg cttcgttcgg cgtgtacgat acggtttcgc gggagttttc gctgatgcgt
     5161 gtccggtatg acgtacaatc ggctatggac cgcatcaaac gggctggtct gccggattac
     5221 ctggcgcacc ggctggaatt tggacgctga tgtccaggca aggaagtggc actatgcgag
     5281 aggcctctgt acagcgtgaa acgaaagaga cacgcattgc tctgaaactc cagctcgatg
     5341 gcgccggggc ggccgatatt cagactgggg tcggtttttt cgatcacatg ctgacacaca
     5401 tcgc
//
LOCUS       DAOPBQ010000222         5356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13089_length_5356_cov_3.372005, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000222 DAOPBQ010000000
VERSION     DAOPBQ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13089_length_5356_cov_3.372005"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PKW60_08270"
     CDS             complement(<1..727)
                     /locus_tag="PKW60_08270"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPJ99464.1"
                     /translation="MLNNYLTRKEQLILLFVAGALVLGGIVWHVRRSDPECETLTTVP
                     VVEAVPTPVAPPELPSQAPPPVSGLSDPAPLIQSQPLRVAVRGAVRVPGLYRFDSGNE
                     PRVQDLLDAAGGLEEYADISDINVAARLLDGSTLTVPGQPPLLDAHGTIRAPRMPACP
                     PPNPPQYTLSGWQPDAPSPVRHSGGPGVESSVSGTSARDGLLDLNSATQAQLESLPGI
                     GPVLASRIIEYRSQTPFRSVEELK"
     gene            complement(921..2162)
                     /locus_tag="PKW60_08275"
     CDS             complement(921..2162)
                     /locus_tag="PKW60_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99465.1"
                     /translation="MKDLRLGFIGAGFIARFQAGALRYVRGVQLAGVYSLDGADALAA
                     QAKEMGLGDCRVYSSVAELCKNVDVVALLAPNYVRVELMEEVAAAVKAGAELKGIICE
                     KPLGRTVAEAKRLVDLVQEAGIPNAYFENQLHMQCIQKALHQLEPQRLSMGELTLARC
                     AEEHSGPHEGWFWDPTRQGGGVLSDMGCHSIAVAWYMLTPVGKPITFMEPVSVTADLA
                     LLKWGQEKYRKQLLERMGVDYAKVPAEDFATGTITFRNPETGQLVRGQFTNSWMYDKQ
                     GLRLLMDGLGPGYTFEINTLRSPLEVFIGDEAAEAVLDAESALEKATASRGLLAVQPN
                     EADLYGYVAELEDAVAAFTEGREPLATWEYGLEITKLCQAAYMAAERRETLDLTDGNM
                     QAKLEGYTSLIAQGKGAEVLM"
     gene            2405..3982
                     /locus_tag="PKW60_08280"
     CDS             2405..3982
                     /locus_tag="PKW60_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007017407.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPJ99466.1"
                     /translation="MRMSWIDVLVLVLYFAAMAAMGPYFARKNKTTENYFLGGRSFPG
                     WLIGLSMFATSISSITFVAYPADAYKTAYLRFLPCLMLPVGIFLASKIFLPFYRRAQI
                     TSAFEYLEVRFGSGTRAYAASAFIIGQVTRLGMILYLVSMLVQEMTGLNAYYSIVLGG
                     VITSFYTITGGIEAVIWTDFFQSFLLWFGGFAILGLIIINVDGGLSTIISVAAADGKF
                     MLGDLNTATGELVRAPRGFSLVEKTALMMLLVGLTNWLTEYSSNQNVIQKYVATKDPK
                     NATQAIWICCLCSVPTWAFFMFLGTALYVYFKLNPDPAAAAMLDGTARADSIVPYFVV
                     QKMPIGLSGLVIAGVLAAAMSSLSSSINAISAVTVVDIYKRHLAKDKSERHYMHAARV
                     VSLIASVLMLGFAFVLLSATDKTLQDTATKLAAILGGGLLGLYFLGFLTRRGDGRAVA
                     VAIVCTLAFSLWLALVEMKVINAVVPFETYYGGLVGNLIMFFVAYILGSVLFPARRDL
                     TNLTVWTQTEEWKSMQK"
     gene            4198..4542
                     /locus_tag="PKW60_08285"
     CDS             4198..4542
                     /locus_tag="PKW60_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPJ99467.1"
                     /translation="MLSEAEDRRYGITEVSALTGVPAYVLRQWEDRFPQLKPGRDRAK
                     RRFYKAADIEIVRRIKQLLWHENMTSKGARVRLAEELRGEGRPRTRKEALDLLDTIEQ
                     QIREMLDLLDNE"
     gene            4555..4632
                     /locus_tag="PKW60_08290"
     tRNA            4555..4632
                     /locus_tag="PKW60_08290"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4590..4592,aa:Pro,seq:ggg)
     gene            4739..>5356
                     /locus_tag="PKW60_08295"
     CDS             4739..>5356
                     /locus_tag="PKW60_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99468.1"
                     /translation="MKIKIPTLQHHRASGRARVSWQGRHIYFGRFGEAETEERYRRWC
                     AELLTGEAQTPERGAGLSVSELAARYMVHARDYYQPDSPMHVMIKNALEPLIRLYGST
                     PAAEFGPKALRTVRGVWLDPERKRVLSRRTVNGYTGCVKRMFRWAASNEFVPVTTYQA
                     LATLDNLRKLRSTARETQSIEPVSQRHLDAVIAVAPRVLRDLIRVH"
BASE COUNT         1032 a         1629 c         1591 g         1104 t
ORIGIN      
        1 ttttaagctc ttcgacactt cgaaagggcg tttggcttcg gtattcgatg atgcgcgagg
       61 ccaataccgg cccgatgccg ggaagtgatt ccaattgagc ctgtgtggct gagttcaggt
      121 cgagcaggcc gtctctcgcc gacgttcctg aaacgctgct ttccacgccg gggccgcctg
      181 agtgtcgcac aggcgaagga gcgtccggct gccatcctga cagcgtgtat tggggcggat
      241 tgggcggcgg acaggcgggc atgcgcggcg cgcggatcgt gccgtgtgca tcaagcaaag
      301 gaggttggcc cggtacagta agggtgctgc cgtcgaggag gcgggccgcc acattgatgt
      361 cggagatgtc cgcgtattct tcgagcccgc ccgcggcatc cagcagatcc tgcacccgcg
      421 gctcgttacc ggaatcaaac cggtacaggc cgggaactcg cacggcgccg cgcaccgcca
      481 cccgcagggg ctgggattgg atcaatggcg cagggtcaga tagcccagag acgggcggcg
      541 gtgcctgaga tggaagctcc ggcggtgcaa caggggtggg tacggcttcc acgacgggca
      601 cagtggtcaa ggtctcgcac tccggatcac ttcgccggac gtgccagacg atgccaccga
      661 gaaccagggc cccggcaaca aagagcagga tcagctgttc ttttcttgtc aggtaattgt
      721 tgagcacggc gccagcccct gctaaggatc tatttaaacg cgtatgcact atgccagaaa
      781 ttactgtagt gcgcaagacg gaccgccctg ggggaggctt cggagaattg cccatcaacg
      841 actttccacg ttgaaaaaag caccgccgcg gatatcggcc cgcggcggtg cgtgcggcat
      901 gctatcgacg ccttgaggtt ctacatcaag acttcggcgc cctttccttg tgcaatgagc
      961 gaggtgtacc cctcgagctt ggcctgcata ttgccgtccg tgagatcgag ggtttctctg
     1021 cgctccgctg ccatgtatgc ggcctggcac agtttggtga tttcgagccc atattcccag
     1081 gttgccagcg gttcccggcc ttcagtgaac gccgccacgg catcttccag ttcggccaca
     1141 taaccgtaca ggtcggcctc gttcggctgc actgccagta gcccacggga ggccgtggct
     1201 ttttccagcg cgctttcggc atcgaggaca gcctcggcgg cttcgtcgcc gataaacacc
     1261 tccagaggcg agcgcagtgt gttgatctcg aaggtatagc caggcccgag cccatccatg
     1321 agcaggcgaa gtccctgttt gtcgtacatc cacgaattgg tgaattgccc ccggaccagc
     1381 tggccggtct cgggatttcg gaaggtgatg gtgcccgtcg cgaaatcttc tgccggcacc
     1441 ttggcataat ccacgcccat gcgctcgagc agctgcttgc ggtacttttc ctggccccac
     1501 ttgagcaatg ccaggtccgc cgtgaccgac acgggttcca tgaaggtgat cggtttgccg
     1561 acaggagtca gcatatacca ggcaacggcg atgctgtggc aacccatgtc ggaaagcacg
     1621 ccgccgccct ggcgggtggg atcccagaac cagccctcgt gcgggccgct gtgctcttcc
     1681 gcgcagcgcg ccagggtcag ttcacccata gacagccgct gcggctcgag ctgatggagc
     1741 gccttctgga tgcactgcat gtgcagctgg ttttcaaagt aggcgttggg aatgccggcc
     1801 tcttgaacga ggtccaccag gcgtttggcc tcggcgaccg tgcggcctag cggcttctcg
     1861 caaatgattc ctttcagttc ggcccccgcc ttgacagccg cagccacttc ttccatcaac
     1921 tcgactcgaa cgtaatttgg ggccagaagc gccactacat ccacgttctt gcacaattcc
     1981 gcgacacttg agtacacccg gcagtcgccg agccccatct ctttcgcctg ggcggcaagc
     2041 gcgtcggcac cgtcaaggga atacacgccg gcaagttgaa ccccccggac ataacgaagc
     2101 gccccggctt gaaaacgcgc aatgaacccc gccccgatga atcccagtct caggtccttc
     2161 atgacacggc ctcccgttta gcacccactt tcgacacagc ccagtataca ggcacaggat
     2221 gcccggtatt caagccatgc cctcagtgtg gcagccctcc tgccgccgtc ctctgcatgc
     2281 ggagggtccg catttgctgt ttcgaggcgt catcagctac catggcgttc cgtgcctgtt
     2341 atcaaactcg tgacagcagt ctcttgcctc caggagagac caacgatctt attggggact
     2401 tcttatgcga atgtcttgga ttgatgtgct cgttcttgtg ctgtacttcg ccgcgatggc
     2461 ggccatgggg ccttacttcg cgcgtaaaaa caagacgaca gaaaactact tcctcggggg
     2521 aaggtcgttc ccgggatggc tcatcggcct gtcgatgttt gccacatcca tcagctcgat
     2581 cacgtttgtg gcgtaccccg ccgacgccta caaaacggcc tacctgcgat ttcttccctg
     2641 tttgatgctc ccggtaggca tctttctggc ctcgaaaatt ttcctgccgt tttaccggcg
     2701 cgcgcaaatc acgtccgcat tcgaatatct cgaggtgcgt ttcggctctg gcacccgggc
     2761 gtatgccgcc agcgccttca tcatcggcca ggtcacgcga ctgggcatga ttctctatct
     2821 cgtgtccatg ctggttcagg aaatgaccgg gctcaatgcg tactacagta tcgtgctcgg
     2881 aggtgtgatc acgtcgttct acacgattac cggcggcatt gaggccgtca tctggaccga
     2941 ctttttccaa tcgtttctac tgtggtttgg cggattcgcc atcctgggcc tgatcatcat
     3001 caatgtagac ggcggcctga gcacgatcat ttccgtggcc gcggcggacg gtaaattcat
     3061 gctgggcgac ctcaacaccg ccacgggaga actcgttcgc gcgcccaggg ggttcagcct
     3121 ggtggagaaa actgcgttga tgatgctgct cgtcggcctg accaactggc tgaccgaata
     3181 cagcagcaac cagaacgtga ttcaaaagta tgtggccaca aaagatccga aaaatgccac
     3241 gcaagccatc tggatctgtt gcctgtgcag cgtgccgacc tgggcctttt tcatgttcct
     3301 gggcaccgca ctctatgtct acttcaaact caatcctgat ccggccgcag cggccatgct
     3361 cgacggcact gcccgggccg attcaatcgt gccgtacttc gttgtgcaaa aaatgcccat
     3421 cggcttgtcc ggcctggtga tcgcgggcgt cttggccgcg gccatgtcgt cactttcctc
     3481 gagcatcaac gctatttctg ccgtgaccgt cgtcgatatc tacaaacgcc atcttgcgaa
     3541 ggataaatcg gagcggcact acatgcacgc cgcgcgcgtg gtctctctga ttgcctccgt
     3601 tctgatgctg gggtttgcct tcgttcttct gagtgcgaca gacaagaccc tgcaggacac
     3661 cgccacgaag ctggcggcca tcctgggcgg cggcctgctc ggcctgtact tcctgggatt
     3721 cctgacccgg cggggtgacg gccgcgcagt agccgtggcg attgtctgca cgttagcgtt
     3781 cagcctctgg ttggcgctgg tcgagatgaa agtcattaac gccgtggtgc cctttgagac
     3841 ctactacggc ggcctcgtgg gcaacctcat catgtttttt gtagcgtata ttctgggcag
     3901 tgtgctcttc ccggcccggc gcgacctcac caacctcacg gtatggacgc agaccgagga
     3961 atggaagagc atgcagaaat aagcgccaaa cgcctgcctt tgaaccccga ttcgggtaag
     4021 gcttgcggca cgccgcacgg agcatgatac tgtgccggcg gccagggcca aaagcggcac
     4081 ggcgcgcccg ggggccgcgt tcgggtgccc ctggcgggcg ggaacagcct tgcatcacac
     4141 ggttccctcg tgagggccgt ttcagctcag agttgcaaaa cgcgtgcagg attcgatatg
     4201 ctctccgagg cagaagaccg gcgatacggg attacggaag tcagcgcgct gactggcgtg
     4261 cccgcctacg tgctgaggca gtgggaagac cggtttccac agttgaaacc aggacgtgat
     4321 cgagccaaac ggcggtttta caaagccgcc gacatcgaga tcgtacgacg catcaaacag
     4381 ctgttgtggc atgaaaacat gacctcaaaa ggcgcgcgcg tacgactggc cgaagagctt
     4441 cgcggcgagg gacgcccccg aacccgcaaa gaagccctcg acctgctcga caccatagag
     4501 caacagattc gagagatgct cgatctgttg gacaacgaat agcctgaatt cggtcggggc
     4561 gtggcgcagc ccggttagcg cgcttgactg ggggtcaaga ggtcgctggt tcaaatccag
     4621 tcgccccgac cattgtaaag gcaaccccct taacgactta cgttaagggg gttgacagtt
     4681 tttgggcgct gtgttagcct gattttaggg taattgtacc ctaaatggag gcgccggcat
     4741 gaaaatcaaa atcccgacgt tgcagcacca ccgagccagt ggccgggccc gtgtctcgtg
     4801 gcaaggccga catatctact tcggccgatt tggcgaggcc gaaacggagg aacgatacag
     4861 gcgctggtgc gctgaactgc tcactggcga ggcgcaaaca ccggagcgag gcgccgggct
     4921 gtccgtttcg gagttggctg cccggtacat ggtgcatgct cgagactact accagcccga
     4981 cagccccatg cacgttatga ttaagaacgc gctcgagcca ctcatacggc tttacggcag
     5041 cacgccggct gccgaattcg ggcccaaggc actgcgcacc gttcggggcg tatggctaga
     5101 ccccgagcgc aaacgggtac tgtcgaggcg caccgtcaac ggctacaccg gctgcgtcaa
     5161 gcgcatgttt cgctgggctg cgtcgaatga gtttgtcccc gtgacaacgt accaggcgct
     5221 tgccactctt gacaatctgc gcaagctgcg tagcacggcg cgcgaaacgc aaagtattga
     5281 gccagtctca cagcgccacc tggacgccgt gatcgccgtt gcgccccgtg tcctgcgcga
     5341 cctgatccgg gtgcat
//
LOCUS       DAOPBQ010000223         5345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13109_length_5345_cov_4.369943, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000223 DAOPBQ010000000
VERSION     DAOPBQ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13109_length_5345_cov_4.369943"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKW60_08300"
     CDS             <1..329
                     /locus_tag="PKW60_08300"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPJ99469.1"
                     /translation="KRIESALRWRDARHRALLEAIPDTLLRIRRDGEILDVHAFDEVP
                     LAGLPPTSPGQHLFDVFPDVIAELAMHHIRRAIDQSRTEAFEFHLDSGDAKGAFEART
                     VPVGPA"
     assembly_gap    350..359
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <360..1034
                     /locus_tag="PKW60_08305"
     CDS             <360..1034
                     /locus_tag="PKW60_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPJ99470.1"
                     /translation="VRLIIRDVSERNRLEREILEVSERERARIGQDLHDGLGQQLVGI
                     GFLVNALAGDLEQGDPGLTENCRHIAAQVSEATEQSRSLARGLHITGLEEDGLARAFE
                     ELAGRTRAVYDVDCRFVCRGAIWLPAEEANQLYRIGQEAITNAIRHARANAITVTLSQ
                     NDRRVRLVIEDDGCGFEPAQDRSSGMGLNIMRYRARVVSANLEVDSEPGHGTRIMCSL
                     PFLRRV"
     gene            1168..1821
                     /locus_tag="PKW60_08310"
     CDS             1168..1821
                     /locus_tag="PKW60_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPJ99471.1"
                     /translation="MKATVLIVDDHPIVRQGLKMLINQADDLEVRGEAENMADALTAM
                     ETLQPDIVIVDISLQGPNGIQLIEAMRQRFPAIPALVLSMHDEWLFAERALQAGARGY
                     VMKQEATDQVVMAIHRVLKGDVYVSAKIAERIVRRVVGGRKPDPESPLEQLTNRELQT
                     LQMLGKGLSTKQIATQLNLSVKTIETYRENLKHKLHLNSSAELIRYAIHRLSEVPLP"
     gene            2712..3251
                     /gene="mraZ"
                     /locus_tag="PKW60_08315"
     CDS             2712..3251
                     /gene="mraZ"
                     /locus_tag="PKW60_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPJ99472.1"
                     /translation="MYFGEYTVKVDDKGRLTVPSRLRQVMEVEGDAVWYLTQGFDGCI
                     SVYPRDEWRRIRTQVNRYSSMNAKALAFRRLFFSSMGEAKIDGQGRMAIPVHLRELGD
                     IGTDVDAILIGVDDHLEIWNRERWRGFQSGHEAVYKEMAALISMGEEMQGVGQAAMMP
                     PHAGGREAYMEKGGKTDAY"
     gene            3241..3585
                     /locus_tag="PKW60_08320"
     CDS             3241..3585
                     /locus_tag="PKW60_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPJ99473.1"
                     /translation="MRIERADLGPVTVLRLAGDLDESGVDALRTAMYECLTQGRFKVV
                     LGLRDVGFISYLGVGVMVERLRKLRDLGGDVKLVGINLYTERLFRMAGVTSLFDVYDS
                     ESQAIGVFQEAA"
     gene            3587..4504
                     /gene="rsmH"
                     /locus_tag="PKW60_08325"
     CDS             3587..4504
                     /gene="rsmH"
                     /locus_tag="PKW60_08325"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPJ99474.1"
                     /translation="MHEPVLAGPAVAWLNVRENGCYVDCTAGAGGHTERIAAELRGGR
                     ILAIDRDPMAVAAARKRLERYSQVEVIHGNYADLSAMLRERGMERVDGVLIDAGVSSM
                     QLDTAARGFSFQETGPLDMRMDTTSDYSAAAFLASETQESLAKRLREYGDVKFPGRIA
                     RTIAAWRAAGQLNSTRDLAGCIREAVPRTALADDEVRRVFQAIRIAVNDELGALERGL
                     RAALEALREGGRLVAISFHSGEDRIVKRLFQEKSRPRQILHADGRVLGQHPPVLRLLT
                     KKPVVPDEAEVRANPRAHSARLRAAERLG"
     gene            4511..4900
                     /locus_tag="PKW60_08330"
     CDS             4511..4900
                     /locus_tag="PKW60_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99475.1"
                     /translation="MASKRRHYRPANVLPAFVKAFPIILIPMGALLCEAWLHLGILTF
                     DYEGRALSMELSRTRAHIEELRARIAELERMERIDAEALELGLVMPEPDQVVTVKEWS
                     AERAWDARQGFDVAHGRIGRSSGQLMD"
     gene            4925..>5345
                     /locus_tag="PKW60_08335"
     CDS             4925..>5345
                     /locus_tag="PKW60_08335"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99476.1"
                     /translation="MSRHADKKPAPNAETSLDRIHRIRIRLVFWALLIVFGVVAGRLV
                     LLQGFPDDIYTREENFHEGTLELNLPRGDIYDRNGRLLATDRRARSLYADPSRIPNPC
                     ELAATLSDLLSEPENEVLARLTRSDSTGRPRKSVPVRR"
BASE COUNT         1065 a         1516 c         1715 g         1039 t
ORIGIN      
        1 gaaagcggat tgaaagcgcc ctgcggtggc gcgatgcccg gcaccgggcc ctgctggagg
       61 cgatcccgga cacgctcctg cggatccgcc gggatggcga aattcttgat gtgcatgcct
      121 ttgacgaggt tcctctggcc ggcctgccgc ccacgtcgcc cgggcaacac ctctttgatg
      181 tgtttcccga tgtcatcgcg gagctcgcca tgcatcatat ccgccgcgcc attgaccagt
      241 cccgaacgga ggcatttgaa ttccatctcg actccggcga cgccaaaggc gcatttgagg
      301 cgcgcaccgt gcccgtcggc ccggcgtagg gcgcgatggc gagccggtcn nnnnnnnnng
      361 tgcggctgat catccgtgac gtgtccgagc gcaaccgcct cgaacgcgag atcctggaag
      421 tcagcgaacg tgaacgtgcg cgcattggcc aggacctgca tgatggcctg ggccagcaac
      481 tcgtgggcat tgggtttctg gtcaacgccc tggcaggcga cttggaacaa ggcgacccgg
      541 ggctgaccga gaattgccgc cacatcgcgg cgcaggtctc cgaggccacc gaacaaagcc
      601 gctcgctggc tcgcggactg catatcacgg ggctggaaga ggatgggctg gccagagctt
      661 ttgaagaact ggcgggccgt acacgagccg tctatgatgt ggattgccgg tttgtctgcc
      721 ggggagccat ctggcttccc gccgaagagg caaatcagct gtaccgtatc gggcaggaag
      781 caattaccaa tgcgatccgg catgcccgcg cgaacgctat cacggtgacc ctgtcgcaga
      841 atgaccgtcg cgtccgcctg gtcattgaag atgacgggtg cggattcgag cccgcacagg
      901 accggagcag cgggatgggg ctgaacatca tgcgctaccg tgcgcgcgtt gtgtccgcaa
      961 atcttgaagt cgattcagag ccgggccatg ggacacggat catgtgttca cttccgtttt
     1021 tgaggcgcgt atgataggaa tcatggacag agcaaggctg gccgtaccgg cggaacgcgg
     1081 gggatatgtt gacgctgccg catacggcgc ctgctcgtgg agcgcaccgg gggctcaagg
     1141 cgtgcacgtg aacggaaaga cgaaatcatg aaagccaccg tgctcattgt cgacgatcac
     1201 cccattgtcc ggcaggggct caaaatgctg atcaaccagg cggacgacct cgaagtccgc
     1261 ggcgaagctg aaaacatggc cgacgcactt accgccatgg agacgttgca gcccgatatt
     1321 gtgatcgtgg acatctcgtt gcagggaccg aacgggattc aattgattga ggccatgcgg
     1381 cagcgattcc cggctatccc ggcactggtg ctctccatgc acgacgaatg gctgttcgcg
     1441 gagcgggcac tgcaagccgg tgcgcgcggc tacgtcatga aacaggaagc aacggatcag
     1501 gtcgtgatgg cgattcaccg tgttctaaag ggtgacgtgt atgtgagcgc caagatcgcc
     1561 gaacggatcg tgcgccgcgt ggtaggcgga cgcaagccgg acccggaatc gcccctggaa
     1621 cagttgacca accgcgaatt gcagaccctg cagatgcttg gcaagggttt gagcaccaaa
     1681 caaattgcaa cgcagctcaa cctgagcgtc aagactatcg aaacctaccg ggaaaacctc
     1741 aaacacaagc tccatctcaa cagcagtgca gaactcatcc gctacgccat ccaccgcttg
     1801 agtgaggtac ctctgccgtg aaaaagcgct gaattgccgg gcagtcaacg gcaaagcgcg
     1861 atttccatac cccggtcaat ggattccctt tcaggcaaaa ccctacagga ttatcaggtt
     1921 ttaacgtata ccaaaaaagg gactgtcctg attacagaca ccatgatgtt gtggttaaat
     1981 caagacggct ggaagatagc ttcgcgggga acaatccccc cgtaagcgta tgaagacgta
     2041 ttcacacagt tgaaatggca gttctgccgc gaggccgtcc ggaggccgat ttccaacgta
     2101 gacagcaggc gcctccgttg acttcgcacc gcgaggaaga tttccccggg tgcaactcca
     2161 gctggccggg tcgggtcttt ttcccggtaa tcagcggcag ccttaacaac gcccccgcag
     2221 cgtggcgtcc ggtgtatgag cagtctgcag gcccctacca ctggacattg tctctgccgg
     2281 acgccacgcc tcccatacat ccctttaccc gcccgggtgt attgtaactt gacctgcccc
     2341 aggattcccc cttttcactg ccgcttcggc atcactcctc ctccgtccac gtaacctgcg
     2401 gcccgtccag agagtgctga atggcgccag gtacagcaag cgcaccgtgc gcagttcatt
     2461 tttcggcact tcttttcagc tgtatcagcg tttggcagct ccgcgtactc catcacctat
     2521 tttgtgcctt atttccgatt tttagtaaat ctataccata tgttgtggtc gcgacctttc
     2581 atttcgccat aaccctttgt gccgcaaagg gaaagtggtt gaaaagggtt gacagtgggg
     2641 caaaatggtg gtagattcgg ggtgctggtg gaaagttgtg gcggaaggtc tctctaccgg
     2701 ggaacgcatt catgtatttt ggggagtaca cagtaaaggt ggatgacaag ggccgcctga
     2761 ctgtgcccag ccggcttcgc caggttatgg aggtggaggg cgacgctgtc tggtacctga
     2821 cgcaggggtt tgacggctgc atttcagtat atccccggga tgaatggcgg cgaatccgca
     2881 cccaagtgaa ccggtattcc tccatgaatg caaaggcgct ggctttccgg cgtcttttct
     2941 tcagcagtat gggggaggca aagattgacg gtcagggccg catggcgatt cccgtacatt
     3001 tgcgtgaact gggggacatc ggcaccgacg tggacgcgat cctgattggt gtcgacgatc
     3061 acctcgaaat ctggaaccgt gaacgctggc gcggattcca gagcggccac gaggcggtgt
     3121 acaaggagat ggcggctctg atttccatgg gcgaggaaat gcagggagtt gggcaggcgg
     3181 caatgatgcc tccgcatgcg ggaggccggg aagcctacat ggagaaaggt gggaagacgg
     3241 atgcgtattg agcgcgcgga tctgggcccg gtgacggtat tgcgccttgc cggcgacctc
     3301 gacgaaagcg gcgtggatgc cctgcgaaca gccatgtatg aatgcctgac gcaggggcgg
     3361 ttcaaagtgg tgctgggatt gcgtgacgtg gggttcatca gctacctggg agttggcgtg
     3421 atggtggaac gcctgcgcaa gctccgggac ctgggcggtg atgtgaaact ggtgggcatc
     3481 aatctctata ccgaacgcct tttccgcatg gccggcgtca cgtcgctgtt tgatgtctat
     3541 gacagcgaat cccaggcgat cggggtgttc caggaagcgg cctagtgtgc atgaacccgt
     3601 tcttgcgggc ccggcggtag cctggctgaa tgtccgggaa aacggctgct acgtggattg
     3661 taccgccggc gcgggcgggc atacggagcg gatagccgct gaactccgcg gcggccggat
     3721 tctggcgatc gaccgggatc ccatggcggt agctgcggca cgcaaacgcc tggaacgcta
     3781 cagtcaggtc gaagtcatcc acggcaacta cgcggacttg tccgcgatgc tccgggaacg
     3841 tggcatggag cgagtggacg gtgtcctgat cgatgcaggg gtgtcaagca tgcaactgga
     3901 cacggcagcg cgcgggtttt catttcagga aacgggcccc ctggatatgc ggatggacac
     3961 cacgtcggat tacagcgcgg ccgcgtttct ggcctcggag acccaggagt ccctggcgaa
     4021 acgcctgcgc gaatacggcg acgtgaagtt tcccgggaga atcgcccgga ccatcgcggc
     4081 ctggcgcgcg gcgggccagt tgaacagcac acgagacctg gcgggttgta tacgcgaagc
     4141 ggtgccgcgg acagctttgg cggacgacga agtgcgccgc gtgtttcagg ccatccggat
     4201 cgccgtgaac gacgagctgg gcgctttgga gcggggcctg cgcgcggccc tggaggcgct
     4261 tcgggaaggc ggccggctcg tggcgatttc gtttcattct ggagaagacc ggatcgtgaa
     4321 acgcctgttt caggaaaagt cgcgcccacg tcagattttg cacgccgatg ggcgcgtcct
     4381 ggggcagcat ccgccggtgc ttcgtttatt gacgaagaag ccggtcgtgc cggatgaggc
     4441 agaggtgcgg gctaaccccc gtgcccacag cgcccgcctg cgggcggcag aacgtttggg
     4501 gtagacagac atggcaagca agcggcgtca ttaccggccg gcgaatgtgc ttcctgcatt
     4561 tgtgaaggct tttcccatca ttcttatccc gatgggggct ctgctgtgcg aggcgtggct
     4621 gcatcttggc attctcacct tcgattacga gggccgggct ctgtcgatgg agcttagccg
     4681 gacccgcgcc catatcgagg agctgcgtgc ccgtatcgcc gagctcgagc gcatggaacg
     4741 catcgatgcc gaggcgttgg agcttggcct ggtcatgccc gaaccggacc aggtggtcac
     4801 agtcaaagag tggtccgcgg aacgcgcctg ggacgcccgg caagggtttg acgtggccca
     4861 cgggcgaatc gggcgaagct caggccagct gatggattga tgcggccgcg taaccgagga
     4921 caagatgagc aggcacgctg acaagaaacc ggctcccaat gcggagactt cccttgaccg
     4981 catacaccgg attcgtattc gcctggtatt ctgggcgcta ttgatcgtct ttggcgttgt
     5041 cgccgggcgt ctcgttctgc tgcagggatt tccagacgat atctacacgc gcgaagagaa
     5101 ttttcacgaa ggcacgctgg agctgaatct tccgcgcggc gacatttacg accggaacgg
     5161 gcgtctgttg gcgacggatc gccgggcccg gtcactgtat gctgatccat cccggatacc
     5221 caacccctgt gagctcgccg ccacgctgtc agacttactg agcgagcctg aaaatgaggt
     5281 gctggcgcgc ctgacccgct ccgactcaac cggccggccg cggaaatcag tccccgtgag
     5341 gcgct
//
LOCUS       DAOPBQ010000224         5336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13135_length_5336_cov_4.179322, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000224 DAOPBQ010000000
VERSION     DAOPBQ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13135_length_5336_cov_4.179322"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PKW60_08340"
     CDS             <1..720
                     /locus_tag="PKW60_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99477.1"
                     /translation="GHLADLQDGTAAPAASPLAAALSQTDKLLETGNYADAYKVLSAA
                     LGRSPDEPDAGDAQMTLAEIAFARLHWYEQAYDDYERLYQKYHQTWLNTPPHVRDRRD
                     LLAEARLVQFRSLQDLDAARANKLDPLDALARVIATYPNQAYVRSQAALEMARRTIES
                     GAVDPQSSDALLQALRHVRNEASDPLVVAQLSYQMGYLYESQYHEFDKAREAFLQASE
                     HPALQQQARSALARLDAESIQ"
     gene            848..1309
                     /locus_tag="PKW60_08345"
     CDS             848..1309
                     /locus_tag="PKW60_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HPJ99478.1"
                     /translation="MRLKRIVSGGQSGVDRAALDVAMRFGFGCGGWCPRGRLAEDGPI
                     PMNYPLKETPSSVYAERTEWNVRDSDGTLVLTWGPPVNGTAFTVDMTRKHGRPVLVID
                     LETRDANLDEVITWLLENGIHTLNVAGPRESKCPGIYEDASAFLEALLSRL"
     gene            complement(1361..2662)
                     /locus_tag="PKW60_08350"
     CDS             complement(1361..2662)
                     /locus_tag="PKW60_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HPJ99479.1"
                     /translation="MARICFLGAGSTVFAKNVLGDCLYEESLRDAQVTLVDIDPDRLH
                     VSEVMMKNICNNLGAKATYTSFLAQDAAKALEGADYVVNAIQVGGYEPATVIDFEIPK
                     KYGLRQTIADTLGIGGIFRGLRTIPVMLEYCRIMERVCPDALMLNYTNPMGILTGALQ
                     RATNIRVVGLCHSVQVCATNLCMMLGLPSDNLKWNIAGINHQGWLLSISRNGEDLYPE
                     IRRRAQLPENRGKDDVRFELMKRFGYYVTESSEHTSEYVPWFIKAKAPELIERFQIPL
                     DEYPRRCVSQIEAWGAMRELLLNEESLHHDRGEEYASYIFGALETGVPYTFGGNVLNK
                     GLITNLPANCCVEVLCVADRSGIHAAHAGELPPQCAALTRTNVNVQELTIEAALTRKK
                     DHIYQAALLDPHTAAELTIDEIVSLCDDLIEAHGASLPSYS"
     gene            2929..3798
                     /locus_tag="PKW60_08355"
     CDS             2929..3798
                     /locus_tag="PKW60_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010276327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPJ99480.1"
                     /translation="MMGVLCSVVVWVTVGAAGSGPESVFFQAHRGGLKEVPENTLAAY
                     RYAWDLGAIPEVDICATKDEVIICLHDDTLKRTTNATVNQDVHVSQLTFEEIRQWDAG
                     SWFDPKFAGEKVPSLEEVLKEMSERKTAQIYLDLKIVDLKALGQLIARYGVANRVIFC
                     HNKVESCRTMREAVPGLRTMLWIGGKPADIQAKFRQAAAEEFEGLNQVQLHLHADPSS
                     ENGTRYLIDEAFLREALTITRDKRIDLEVLPFAFDCASLSGLLDLGIRWYATDEPKRF
                     KACVREHFGVAGE"
     gene            complement(3856..4005)
                     /locus_tag="PKW60_08360"
     CDS             complement(3856..4005)
                     /locus_tag="PKW60_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99481.1"
                     /translation="MPEHWFWFLATAVCVGWYSTITIYVAIRGALDIKNMLRRLAGDS
                     GEASE"
     gene            complement(4008..>5336)
                     /locus_tag="PKW60_08365"
     CDS             complement(4008..>5336)
                     /locus_tag="PKW60_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPJ99482.1"
                     /translation="AATEFGIVTCMYTSQNGYKNGFAGATPGILAMLAMFFVGYTGFC
                     VKPLRDAGVITIPELFEVRYGPRIRWAAGVVIVLGGLLNMGVFLRTGGDFLVAVCGFD
                     PKYLEITMTALLVFVAVYTVLGGMLSVLVTDFLQFVVMSGGLLAVTVLILVNIGWSDL
                     VAAVQKHHGAGGFNPLIKPEMGWKYIADNALLSLAMVLTWQTTIQRLLAAKDTRTGQQ
                     VYTRTSVFFLCRWLIPVLWGIAALAILDPLEVGDNTLLAMPMMLSRITPVGLMGILVA
                     AMLAADMSTDAAYMLTWSSVIYNDILAPFHKRQWSEKRGLIVSRSLVAIIGVFLLLYG
                     LWYPLQGSLWDYLRVTGNIYLSSMSVLLVACCYWKRANSWGAAGAIVCGAVIPTAFLV
                     MEQLPSTQHFAKVVVGPEYSNIATYIIATTAMVVGSLLKPQTKAVAAKGY"
BASE COUNT         1126 a         1561 c         1598 g         1051 t
ORIGIN      
        1 ggccatctgg cagacctgca ggacgggaca gctgctcctg ctgcctcgcc tctggcagcc
       61 gcattgtccc agaccgacaa gcttcttgaa accggcaatt atgcggatgc gtataaggtg
      121 ctttcagcgg cactcggccg cagtcctgac gaaccggatg ccggcgacgc ccagatgacg
      181 ctggccgaga tcgcgtttgc ccgcctccat tggtatgaac aggcctacga cgattacgag
      241 cgtttgtatc aaaaatatca tcagacctgg ctgaatacgc cgcctcatgt tcgggaccgg
      301 cgcgatctgc ttgcggaagc gcgtctggtg cagttccgct cactgcagga cctggatgcc
      361 gcacgcgcca ataaactcga cccgcttgac gcgttggcgc gggtgatcgc cacgtatccc
      421 aaccaggcct acgtgcgctc gcaggccgcc ctggaaatgg cccggcgcac tattgaatca
      481 ggcgctgtcg acccgcaatc ttcagatgcg ctgctccagg cgctgcgaca cgtgcgaaac
      541 gaggcctcag atcctctggt tgtcgctcaa ctgtcgtatc agatggggta cctctacgaa
      601 tcgcaatacc acgaattcga caaagcccgt gaggcttttc ttcaggcgtc agagcatccg
      661 gcgcttcagc agcaggcgcg ctctgccctt gcccgcctcg atgcggaatc catacagtaa
      721 cgctccaggg caatcgcgag gcatccggcc gccccggcag gtacaatagc cagcgcagag
      781 aaaaatctct ggagagcgaa cagtgtcaga gtttccggcg ccagcctggc ggcaagccga
      841 ggatctcatg cgactcaaac gcattgtgtc gggaggacaa agcggcgtgg accgtgccgc
      901 actggatgta gccatgcgct tcggcttcgg ctgcggggga tggtgtcccc ggggccgcct
      961 cgccgaggac ggtccgatcc cgatgaatta tcccctcaaa gagactcctt cgtccgtgta
     1021 tgccgaacgg accgagtgga acgtcaggga ttcggacggc acgcttgtgc tcacgtgggg
     1081 acctccggtc aacggcaccg catttacggt ggatatgacc cgaaagcacg gacgccccgt
     1141 gctcgtgatt gacctcgaaa cgcgagacgc caacctcgat gaggtgatca cgtggctcct
     1201 ggaaaacggc atccacacgc tgaatgtcgc cgggccgcgt gagagtaaat gcccagggat
     1261 ttacgaggac gcgtcggcat ttcttgaagc gttgctctca cgcctgtaag cagcccggaa
     1321 ttctacagcc cacgcctttt tcataagcag cagcggcagc tcacgagtag gaggggagac
     1381 tggcgccatg ggcctcgatc aaatcatcgc agagtgagac gatttcatcg atcgtgagtt
     1441 ccgctgcggt atgcggatcg agtaatgcgg cctggtaaat gtgatccttc ttacgggtaa
     1501 gggccgcctc gatggtcagt tcctggacgt tgacgttggt gcgggtgagt gcggcgcact
     1561 ggggcggcag ttcgccggca tgggccgcat gtatgccact acggtcggca acgcatagca
     1621 cttcaacgca gcaattggcg ggcaggttgg taatcaaccc tttgttcagc acattgcctc
     1681 cgaaggtgta tggcacgccg gtttcgagcg ccccgaagat gtaggaggca tattcttcgc
     1741 cccggtcgtg atggagggat tcctcattga gcagcagttc cctcattgcg ccccaggctt
     1801 cgatctgcga gacgcagcgc cgcggatatt catccagcgg gatctggaac cgttcgatca
     1861 gctccggggc cttcgctttg atgaaccagg gcacgtattc gctggtgtgt tcactcgatt
     1921 ccgtaacgta atacccgaac cgtttcatca attcgaagcg cacgtcgtcc ttgccgcgat
     1981 tttccggcaa ctgagcacgg cgccggattt ccgggtacag gtcctcgccg ttccgtgaga
     2041 tgctcagcaa ccatccctgg tgattgatcc ccgcaatgtt ccatttcagg ttgtcgctgg
     2101 gcaggccgag catcatgcac aggttggtgg cgcagacctg cacgctgtgg cacaggccga
     2161 caacgcggat attcgtggcc cgttgaagcg cgcccgtcag aatgcccatc ggattcgtgt
     2221 aattcagcat cagggcgtcc ggacacacac gttccatgat ccggcaatac tcgagcatga
     2281 ccgggatcgt gcggagccct cgaaagatgc cgccgatgcc gagggtgtcg gcgatggtct
     2341 ggcgcaggcc gtactttttg gggatctcaa aatcgatcac cgtggcgggc tcatacccac
     2401 ccacctggat cgcattgacc acgtagtccg cgccttcgag cgcttttgcg gcgtcttgcg
     2461 ccagaaagga agtgtacgtt gctttcgcac cgagattatt gcagatgttt ttcatcatga
     2521 cctcggagac gtgcagccga tccgggtcga tatccaccag tgtcacctgg gcgtcgcgga
     2581 ggctttcttc atacaggcag tctccgagca catttttcgc aaacacggta cttccagcgc
     2641 ccaaaaaaca aattcttgcc atgacagact cctccattgc cagatgcaaa tgcatgatac
     2701 gttcagcggc gaaatgagat caaagaacaa gtctccagcg ctgctgttgt agactgccgg
     2761 tcgattctta ccgccgcgag cgtcgagtcg ggttttctgt cacgggtttg gtttgtgtag
     2821 aataacttga gcccagcgag gccctgcctg aatggggcag cagactttgt gcggccactg
     2881 acacgcaggc aggcccgcag aggtgtgaga ggcaacagga ggtacgtcat gatgggcgtt
     2941 ttgtgcagcg tggttgtgtg ggtcactgtt ggtgctgcag gttccggacc agagtcagta
     3001 ttttttcagg cgcatcgcgg tggattgaaa gaagtgccgg agaacacgct ggctgcgtac
     3061 cgctacgcct gggatctcgg agcgatcccc gaggtggaca tctgcgcaac caaagacgag
     3121 gtgatcatct gcctgcatga cgacaccctg aagcgcacaa ccaacgccac cgtcaaccag
     3181 gacgtccatg tgtcgcagct gaccttcgag gagatccggc aatgggacgc cggcagctgg
     3241 tttgatccca aattcgcggg agagaaggtc cccagcctgg aagaagtgct gaaggagatg
     3301 tccgagcgca aaaccgcgca aatctacctt gatctgaaaa ttgtcgacct gaaagcactg
     3361 ggccagctga tcgcgaggta cggcgtggcc aatcgagtga ttttctgcca caacaaggtc
     3421 gaatcgtgcc gtacgatgcg tgaagccgtg ccgggactgc gtaccatgct gtggatcggc
     3481 ggcaaaccgg ccgacatcca ggcaaagttc cgccaggcgg ctgcggagga gttcgaggga
     3541 ttgaaccagg tgcaacttca tctacacgct gatccatcgt ccgaaaacgg cacccggtat
     3601 ctgatcgatg aggcatttct ccgggaagcg ctgacgatca cacgggataa gcgcatcgac
     3661 ctggaggtgt tgccgtttgc gttcgattgc gccagtcttt caggcctgct cgacctgggc
     3721 attcgctggt acgcgaccga cgagcccaaa cggttcaagg cctgcgtgcg cgagcatttc
     3781 ggcgttgccg gggaatgacc cggcaacggt acggatcagc tgatatccag attcgttgtg
     3841 attccgctcc ggaccctatt ctgatgcttc gccgctatcg ccagccaggc ggcgaagcat
     3901 atttttgatg tccagcgcac cgcgaatcgc gacgtaaatg gtgatggtcg agtaccagcc
     3961 cacgcacacg gccgtcgcca gaaaccagaa ccaatgttca ggcatggtca gtatcctttc
     4021 gctgcaacgg cttttgtctg gggtttcagc agcgagccga ccaccatggc cgttgtggca
     4081 atgatgtacg ttgcgatgtt ggaatattcg gggccaacga ctactttggc gaaatgctga
     4141 gtgctgggca gctgttccat gaccagaaag gcggtcggga tgacagcgcc acacacgata
     4201 gcgccggccg cgccccagct attggcccgc ttccagtagc agcatgccac cagcagcacc
     4261 gacatgctgg acagatagat gttcccggtg acccgcagat aatcccaaag gctgccctgc
     4321 aaggggtacc acaggccgta cagcagcagg aagacgccga tgatggcgac cagcgagcgg
     4381 ctgacaatca aaccgcgctt ttccgaccat tgccgtttgt gaaatggcgc caggatgtcg
     4441 ttgtagatga cgctggacca cgtgagcata taggcagcat cagtcgacat gtcggcggcc
     4501 agcatggcag ccaccaggat gcccatcaga cccacggggg tgattcggct cagcatcatc
     4561 ggcatggcga gcagggtgtt gtcgccgact tccagcggat ccagtatcgc aagggccgcg
     4621 atgccccaca gcacggggat gagccagcgg cagagaaaga agacactggt gcgggtgtac
     4681 acctgttgcc cggtcctggt gtctttcgca gccagcagcc gctggatggt cgtctgccag
     4741 gtcaggacca tggccagact gagcagagcg ttgtcggcga tgtatttcca tcccatttcc
     4801 ggcttaatga gcgggttgaa ccctccggca ccgtgatgct tttgaactgc ggccaccagg
     4861 tcgctccaac cgatgttgac caggataagg acggtcacgg cgagcaagcc gccgctcatc
     4921 accacaaatt gcagaaagtc tgtgaccagc acggacaaca tgccgcccag gacagtgtaa
     4981 accgccacaa agaccagaag cgcggtcatg gtgatctcga gatatttcgg gtcaaacccg
     5041 cataccgcca cgaggaagtc gccgccggtg cgcaggaaga cgcccatgtt gagcagtccg
     5101 ccgagcacaa tgaccacacc ggccgcccac cggatgcgtg gaccgtatcg cacttcgaac
     5161 agttccggga tggtgatcac gccggcatcg cgcagcggct tgacacaaaa gccggtgtac
     5221 ccgacaaaga acatggccag catggcaagg atgcccggcg tagcgccggc aaacccgttc
     5281 ttatacccgt tctgcgacgt gtacatgcac gtcacgatgc cgaattcggt ggccgc
//
LOCUS       DAOPBQ010000225         5333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13144_length_5333_cov_3.551724, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000225 DAOPBQ010000000
VERSION     DAOPBQ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13144_length_5333_cov_3.551724"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /gene="bshC"
                     /locus_tag="PKW60_08370"
     CDS             complement(<1..331)
                     /gene="bshC"
                     /locus_tag="PKW60_08370"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HPJ99483.1"
                     /translation="MRELYDEYLSESPDLMPFYAAPPSRLEESLPRPVAWDQMLVREV
                     RAYNERIGSGGAFDGTELVVATGQQPGLFSGPLYTVYKAITCIMLARQLEERSGTPCV
                     PLFWVASE"
     gene            complement(336..1091)
                     /locus_tag="PKW60_08375"
     CDS             complement(336..1091)
                     /locus_tag="PKW60_08375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPJ99484.1"
                     /translation="MTDFPEKIAPDWYARAFDSLYPILYAHRTVEAARPEAEFAAAKL
                     GLGGTEQVLDLCCGNGRHMCHLRSRCGALAGLDYSEALLRLARDSVGEQCMLVRADMR
                     AIPFNQTFDAVTNFFTSFGYFFDPEDNLKVVCGVAAALKPRGRFFIDYVNRESAIAQL
                     VPESVRTRGNYEMHDRRWVDFEQNRLNKLTTVRYDGALIREVEESVQLYSPGEFEDLL
                     RSGGLKLEAFYGDYTGAGLDSNAPRMIACGSRE"
     gene            complement(1095..2543)
                     /gene="pyk"
                     /locus_tag="PKW60_08380"
     CDS             complement(1095..2543)
                     /gene="pyk"
                     /locus_tag="PKW60_08380"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015170465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPJ99485.1"
                     /translation="MRRTKIVCTLGPSSNDLPTIERLLSEGMDIARLNFSHGTHDDHR
                     IVFEAIRQAAARLGKPVAVMADLQGPKIRTGRLAGGKPVLLQPGAPICITTREVPGDA
                     QCVSTTYDALPRDVAPGDRLLLADGMLELAVVEVKGPDVHCTVLRGGELGQSKGVNLP
                     GVAVSAAAFTAKDAEDLEFALGLGVDYVALSFVRSADDIRALKARIAQIGHETPVVAK
                     IERPEAVAAIDDIIAVADAVMVARGDLGVEMALDEVPQVQKDVVRKCNRNGVPVITAT
                     QMLESMMASARPTRAEVADIANAIHDGTDALMLSGETAVGRYPVEAVITMAQVAKKTD
                     EVVEQLPRLSPAIPDTISRVDSTFSHALGLAASRAAEDVNAKCITCFTMTGYSARMIS
                     RQRPRKPVVAVTMSEETRRRCALYWGVQTLEGEETESLNAMVKQVDEILSREGLARQG
                     DTVIIVGGSPLLIGGRANFLKLHQLGESETRR"
     gene            complement(2550..3863)
                     /locus_tag="PKW60_08385"
     CDS             complement(2550..3863)
                     /locus_tag="PKW60_08385"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPJ99486.1"
                     /translation="MGLITCKFGGSSLADANCFRKAAAIVRANPDRRFIVPSAPGKRT
                     DDDKKITDLLYAWHSLAQQGLDASQPRDIIATRFSELAAELGISFDVDMHMARIASEG
                     EQRAEPDYMASRGEYLSGLLLAEFLGAAFVDPAECVLFNDEGMLDSRTYGALGARLSG
                     DGLWVVPGFYGAMPDGRIRTFSRGGSDITGAIVARASQSALYENWTDVSGFRMADPRV
                     VPQARRIGEVTYRELRELSYMGAKVLHDEAIFPVRETGIPVNIRNTHNPDSPGTMIVA
                     SRKSSEPVCGIAARKGFSMITIEKALMNHELGFGRRVLMILEEFGVSFEHMPSSIDTI
                     SVIVRDDQLGDRGPAVLRAIERQCEPDRIDLVPGLALIATVGQGMAGHVGIAAKLTGA
                     LAKARVNIRVLDQGSSENNIIVGVEDGDMETSVRAIYEVFASTQE"
     gene            complement(4083..>5333)
                     /locus_tag="PKW60_08390"
     CDS             complement(4083..>5333)
                     /locus_tag="PKW60_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPJ99487.1"
                     /translation="PAASAPLYRVPSTGDSENLSREPDGQGWKTGDKIISIDGKSVHS
                     FSDVSFAAILGAGKTLDVLIERTEQGGPVRYLSRIEPKLLDGGERTQFGVAPFQTALV
                     DRVLPDLPAAEAGLQAGDIIQRVNGQLIDTATFVKFLENLKSGDSVVLDVHRGGENLS
                     YTLQPATLGRLEGVVFYPSLYSDAESDQDTPPTIGYVTDEVAERTGLRRGDVILEVNG
                     EPATAKTMREAERNNAGHSLTLKVQQQAVLAGLRQRAEIKTIEVTPEPAGQIGIIWGQ
                     ILVHYRVPPLRVLPEAVREGYSALSKTVETLVMLFAGTVKPKDLGGPVLIYQATTTAA
                     SMGLTWLFKITAFISVNLCVFNLLPLPVLDGGHLLFLTWEGVRRKPLPVKAQERIQQA
                     GLVLIVSLLLFVTYNDILRWFASL"
BASE COUNT         1020 a         1715 c         1539 g         1059 t
ORIGIN      
        1 cttcgctggc tacccagaac agaggcacac acggcgtgcc ggagcgttcc tcgagctgcc
       61 gggcgagcat aatgcaggtg atcgccttat acaccgtgta caacggcccg gaaaacaggc
      121 ccggctgctg cccggtcgcg acaacgagct cggttccgtc aaacgccccc cccgaaccga
      181 tgcgctcgtt gtacgcgcgc acttcacgta cgagcatttg gtcccaggca acgggacggg
      241 ggagggattc ttccaatctg gacggtggcg cggcgtaaaa cggcattaaa tcaggtgatt
      301 cgctcaaata ttcgtcatac agctcgcgca acctctcact ccctgcttcc gcatgcaatc
      361 atgcgcgggg cgtttgaatc cagcccggcg ccggtgtaat caccgtaaaa tgcctccaat
      421 ttcaggccgc ccgaacgcaa aaggtcttca aattcgcccg ggctgtacaa ttgcaccgat
      481 tcttcgactt cacggatgag cgccccgtca taccgcacgg tggtaagctt gttcaggcgg
      541 ttttgctcga aatcgaccca gcggcggtca tgcatttcgt agttcccccg cgttcggaca
      601 ctttcgggca ccagttgggc gatagcactc tcccggttca cgtagtcgat aaagaaccgc
      661 ccgcgcggct tcagagcggc cgccaccccg cagactacct tgagattgtc ctccggatca
      721 aaaaagtagc cgaacgaggt aaagaaattg gtgaccgcat cgaaagtttg attaaaagga
      781 atcgcccgca tgtcggcccg gaccagcata cactgttcac ccacggaatc acgggccagc
      841 cgaagcagcg cctccgaata gtcgagccct gccaaagcgc cgcacctact ccgcaagtgg
      901 cacatgtgcc ggccgttacc gcagcacaga tcgaggacct gttccgtgcc gcccaggccc
      961 agtttggccg cagcgaactc cgcctccggc cgggccgctt caaccgtgcg gtgggcgtac
     1021 aggatggggt agaggctgtc gaaagcccgg gcataccagt ccggcgcgat tttttccggg
     1081 aaatccgtca tgccttaccg cctggtttct gattccccca gctggtgaag cttgaggaaa
     1141 ttggcacggc ccccgatcaa tagcggcgag cctcccacaa tgatgaccgt atcgccttgc
     1201 ctggccagcc cttcgcggct cagtatttcg tccacctgtt tgaccatggc attgaggctc
     1261 tccgtttctt cgccttccag agtctgcacg ccccagtaca gggcgcagcg gcggcgggtt
     1321 tcctccgaca tcgttaccgc gacgaccggt tttcgagggc gctgccgcga gatcatccgt
     1381 gcactgtaac ctgtcatggt aaaacaagtt atgcacttgg cgttcacatc ttccgcagcc
     1441 cggctcgctg ccagtccgag cgcgtgggag aacgtcgaat cgacacggga gatggtatcc
     1501 ggaatcgcgg gggagagacg cggcagctgt tccacgacct cgtcggtttt tttggccacc
     1561 tgggccatgg tgatcacagc ttcaacgggg tagcgaccga cggcagtctc accgctgagc
     1621 atcagcgcgt cggtgccgtc atggatggca ttggcgatat ccgcgacctc cgcgcgggtc
     1681 ggccgggcgc tggccatcat ggattccagc atttgcgtgg cggtgattac cggcaccccg
     1741 ttccggttgc acttgcgaac gacgtctttt tgaacctgag ggacttcgtc cagggccatt
     1801 tcaaccccca ggtcaccccg ggccaccata accgcatcgg ccacggcgat gatatcatcg
     1861 attgcagcaa cggcttccgg gcgttcgatc ttggccacca cgggagtctc gtgtccgatt
     1921 tgcgcaattc gtgctttcaa tgcgcggata tcgtccgcag agcgcacgaa cgaaagggcc
     1981 acatagtcga cgcccagtcc cagggcaaat tcgaggtctt ccgcgtcctt agccgtgaat
     2041 gcagccgcac tgacggcgac gccgggcaga ttgacgccct tggattggcc cagctcgcca
     2101 ccgcgcagca ccgtgcaatg aacgtccggc cccttcacct cgaccacggc caattcgagc
     2161 atgccatccg caagcaggag ccggtcgccc ggagcgacat cacggggcag cgcgtcatag
     2221 gtggtcgaga cgcactgcgc gtcgcccggg acctcccgcg tcgtgatgca gatcggagcg
     2281 ccgggctgca ggagaacggg tttccctccg gcgagccgcc cggtacggat tttcggcccc
     2341 tgcaggtcgg ccatcacggc tacgggtttg ccgagcctgg ccgctgcctg gcgaatcgct
     2401 tcgaatacga tgcggtggtc atcgtgggtg ccgtgcgaga aattgaggcg cgcgatgtcc
     2461 atcccctcgg ataaaagccg ctcaattgtt ggtaaatcgt tcgagctggg tcccagagtg
     2521 cagacgattt tggtccgtcg catagcggct tactcctggg tgctggcgaa cacctcgtag
     2581 attgcgcgca ccgaggtctc catatcgccg tcctcgacac ccacaataat gttgttttcg
     2641 gaagatccct ggtccagcac gcggatattc acccgggctt tggccagtgc ccctgtaagt
     2701 ttcgcggcaa tccccacatg tccggccatt ccctgaccca ctgtcgcaat aagcgccagc
     2761 cccgggacca gatcgatccg gtcgggttcg cattgacgct cgatggctcg aagcaccgca
     2821 gggccgcgat cgcccagttg gtcgtctctg acaatgacgg agatcgtgtc gatgctcgac
     2881 ggcatgtgct caaaactgac tccaaactcc tccagaatca tcagcacgcg gcggccaaac
     2941 cccagttcat ggttcatgag cgccttttcg atggtgatca tcgagaagcc tttgcgcgcc
     3001 gcgatcccgc agacgggttc gctgctctta cgcgaggcga cgatcatcgt gcccggggag
     3061 tccgggttgt gcgtgttacg gatattgacg gggatgccgg tttcgcgcac ggggaaaatg
     3121 gcttcgtcat gaagcacctt ggcgcccatg tacgacagct cccgcagctc ccggtacgtc
     3181 acttccccga tacggcgcgc ctgggggaca acccggggat cagccatccg gaatcccgat
     3241 acatcggtcc agttttcgta cagggccgac tggctggcgc gagccacgat ggccccggta
     3301 atatcgctgc caccgcgcga aaacgtccgg atccgcccgt ccggcatagc gccgtaaaaa
     3361 cccggcacca cccacagacc gtcgcccgac aaacgggccc ccagtgctcc gtacgtcctg
     3421 gaatcgagca ttccttcgtc attgaacagg acacattcgg ccggatcaac aaaggccgcc
     3481 ccgaggaatt cggccagcag caacccgctg aggtattccc cccgtgacgc catatagtcc
     3541 ggttcagcgc gctgctcgcc ttcggatgca attcgcgcca tgtgcatatc cacgtcgaag
     3601 gagatgccca gctcggcggc cagctcgctg aagcgggtgg cgatgatatc ccgcggctgg
     3661 gacgcatcca ggccctgttg agccaggctg tgccacgcgt acaggaggtc cgtaatcttc
     3721 ttatcgtcgt cagtacgttt tcccggcgcc gacggcacaa taaaacgccg gtccggattg
     3781 gcccggacaa tggcggcggc tttgcggaaa caattcgcat ccgccagcga agatccaccg
     3841 aatttgcagg tgatcagtcc catggtctac cgatttctcc ttccttcgcg catgccgcgc
     3901 ggctggtatc gacgctgtat tcagccgggg acgcatggcg gcgccacgca cagcgcccgg
     3961 gacatgtccc cgttttccgc tttcatttgc gatacgacgc cggatgggcc cgcgcacttc
     4021 gccctgtttt cattgtttac gggcgagcca gcgggcgtgt gcacggttca ctccagccac
     4081 actcaaagcg aagcgaacca gcgtagaatg tcgttgtacg ttacaaacag cagcaggctg
     4141 acaatcagca ccagccccgc ctgctgaatg cgttcctgtg ctttcaccgg aagcggtttc
     4201 ctccgcacac cctcccaggt tagaaacagc aggtgccccc cgtccagaac cggcaggggc
     4261 aacagattga acacacaaag gttcacgctg atgaatgccg taattttgaa cagccacgtc
     4321 agccccatgc tcgctgccgt cgtcgtcgcc tgataaatca agaccgggcc gcccaggtct
     4381 tttggcttaa ccgttcccgc gaagagcatc accagcgttt cgaccgtctt cgacagggcc
     4441 gaatatccct ctcgaacggc ctctggaagc acccggagcg ggggaacacg gtagtgtacc
     4501 aggatttgcc cccagataat cccgatctgc ccggcaggct cgggcgtgac ttctatggtt
     4561 ttaatctccg cccgctggcg caggccggcc agcactgctt gctgctgcac cttgagcgtc
     4621 agggagtggc ccgcgttatt gcgttcggcc tcacgcatcg tcttagccgt tgcgggttcg
     4681 ccgttcactt ccagaatgac atcgccccgc ctcaagcccg tgcgttcagc tacttcatcg
     4741 gtcacgtacc cgatcgtggg cggggtgtcc tggtcacttt ccgcgtcgct gtacagcgaa
     4801 gggtagaaaa cgacgccttc cagccggccg agcgtggccg gttggagcgt gtaggagagg
     4861 ttttcgccgc cccggtggac gtcaagaacc acactgtctc cacttttcag gttttccaga
     4921 aactttacaa aagtcgccgt gtcaatgagc tgcccgttga cgcgctggat aatatcaccc
     4981 gcctgaagtc cggcttccgc ggcgggcaga tccggcagga cgcgatccac cagggcggtc
     5041 tggaacggcg cgacgccaaa ttgcgtgcgt tcgcctccat cgagcagctt tggctcgatc
     5101 cgcgacaaat agcggactgg tccgccctgt tcagttcgtt cgatgagcac atccagggtt
     5161 tttcctgcgc ccaggatggc ggcaaagctg acatctgaaa aactatgcac cgattttcca
     5221 tcaatcgaga tgatcttgtc gcctgttttc catccctggc cgtcgggctc gcgcgagagg
     5281 ttttcggaat ctcccgtaga ggggactcga tagagaggag cgctggccgc cgg
//
LOCUS       DAOPBQ010000226         5331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13147_length_5331_cov_4.030136, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000226 DAOPBQ010000000
VERSION     DAOPBQ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13147_length_5331_cov_4.030136"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /gene="ilvB"
                     /locus_tag="PKW60_08395"
     CDS             <1..1526
                     /gene="ilvB"
                     /locus_tag="PKW60_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HPJ99488.1"
                     /translation="AGFIAQGMARATGTPAVFLATSGPGATNALTALADAKLDSVPII
                     CITGQVPRAMIGTDAFQEVDTYGMSIPLTKHNFLVRSAAALLKVLPEAFRIAASGRPG
                     PVLVDVPKDVQTETVSFESWPEPGCADAPPDFDPAAVSLAAEFINGAERPVLYLGGGV
                     VYGGASEYAIQLAEKASLPTTMTLMGLGAMPPEHPLSLGMLGMHAARFTNLVMEECDL
                     LIAAGARFDDRATGKAAEFCPNAKIVHIDIDHSELDKVKTAHAGIVGDVRAVLERLLP
                     LIQANPRPEWLERVDELRRAFPMDMPGLDDARTPYGLIAETARHLDDSAIVTTDVGQH
                     QMWAAQAYPLRRPRQWLTSGGLGTMGFGLPAAIGAALACPERDVVCISGDGSLLMNIQ
                     ELVTAAEENANVKIIVMNNNSLGLVHQQQDLFYGKRIFASDFVVAVDFCMIARGFGMR
                     TADLGATDDPAGMLAEAMTTPGPCLINAPIDVNEKVYPMVPPGAANRTMVAYETVAG"
     gene            1610..1924
                     /gene="ilvN"
                     /locus_tag="PKW60_08400"
     CDS             1610..1924
                     /gene="ilvN"
                     /locus_tag="PKW60_08400"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPJ99489.1"
                     /translation="MSIATESAAISALPRTVLELTVNNHPGVMSHVCGLFARRAYNVE
                     GILCMPIGDEASSRIWLLVNEDARLDQMIKQAEKLHDVRAVRRHPATREVFEQLEAFF
                     RE"
     gene            2161..3219
                     /gene="ychF"
                     /locus_tag="PKW60_08405"
     CDS             2161..3219
                     /gene="ychF"
                     /locus_tag="PKW60_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPJ99490.1"
                     /translation="MRVGIIGFARSGKTTVFNSLTGARAEVGAFGSREANLAVLKVPD
                     ERVDRLAEIYEPKKVTYAEFQFVDIAPDEAAGEEKALSSAALTALRQVEALVHVVRAF
                     TGETVVHPLGRVDAARDCRALEEEMQLDDLIIIERRLERMEKENRKDRQYELLARCKA
                     HIEAGKPLRTLELSALEEKDIAGFCFLSRKPLLLLGNYGDERIGDADPAKLHALAAET
                     GQTLIELCGAMEMEVSQLPEDERAAFREELGLGEGSRTRFLKSAYAMLGLISFLTAGK
                     PEVRAWTIRKGTRAQDAAGVIHSDLARGFIRAEVTHYDDFVNAGSMAKAKEAGLLRLE
                     GKDYIVQDGDIMLIRFNV"
     gene            3433..4413
                     /locus_tag="PKW60_08410"
     CDS             3433..4413
                     /locus_tag="PKW60_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99491.1"
                     /translation="MNVGVIGCNTMGLMHCEMAVNAGLKVVACGDISRDNARKAARKF
                     GAEAVGDCVELCSRDDVDVVAICTPTPTHTEYIKAAAAAGKHVFCEKPLGRTLEKIDE
                     ALAAVEQAGVKLYVGHVVRFFQEFEAMKARIDSGQIGKVGFVKTYRGGIFPGGKLGWF
                     SDWSMSGGVTLDTIVHDMDWLRYVFGDPERIFAQNLKRVFSERLDYALVTMRMKSGVI
                     CNVVGTWAHPSGFRVKVDICGERGMVTFDSSEAPVSAQLRQGAGSGPSTIVPASPVLV
                     SPYQLEWEDFAGWLAGTHEPRVTPEDGRWAVRMALGALESAETGEPVEFN"
     gene            4449..>5331
                     /locus_tag="PKW60_08415"
     CDS             4449..>5331
                     /locus_tag="PKW60_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99492.1"
                     /translation="MAKIGVMSFAHMHAQSYAACLNELPEAELVAIWDDDAARGQQQA
                     EAFRTDFVPDLDAFLARDIDGVIIASENVKHREMVERAAAAGKWILCEKPLAPTVEDC
                     QAMIEACRKAGVGLGTAFPCRYIPSVMAVKDELDKGVYGEIYAASCTNNGQFPGGWFA
                     QQALSGGGAVMDHTVHVADLLRWMLNREFTRVYCENGRLLRPEIDVDDVGSLHLEMEG
                     GVIVSHIASWNRAASFPTWGDITLELIGEKGVVSVDAFNQKLNVYDDTAMRAAWDYWG
                     GSPELGLVKAFGEAVDAK"
BASE COUNT         1010 a         1466 c         1893 g          962 t
ORIGIN      
        1 gcgcggggtt cattgcgcag ggcatggccc gcgccaccgg gacgccggcg gtgtttctgg
       61 cgacgtccgg gccgggggcg acgaacgccc tgaccgcgct ggccgacgcc aagctggatt
      121 cggtgccgat catctgcatc acgggccagg tgccccgtgc gatgatcggt acggacgcgt
      181 ttcaggaagt cgacacctat gggatgagca tcccgctcac aaaacacaac tttctggtcc
      241 gctcggcggc ggcgctgttg aaagtcctcc ccgaggcgtt ccggatcgcg gcttcgggcc
      301 ggcccggccc ggtgctggtg gatgtgccga aagatgttca aactgaaacg gtgtcgttcg
      361 aatcgtggcc cgaacccggg tgtgctgatg cccccccaga cttcgatccg gcagctgttt
      421 cacttgcggc agaattcatc aacggggcag agagaccggt gctgtacctc ggcggcggcg
      481 tggtgtacgg cggcgcgtcg gaatatgcga ttcaactggc ggaaaaggcc tcgcttccca
      541 cgacgatgac gctgatgggt ctgggcgcga tgccgcccga gcatccgctg tcgctgggca
      601 tgctggggat gcacgcggcg cgattcacca acctggtcat ggaagagtgc gacctgctca
      661 tcgcggcagg cgcgcgcttt gacgaccggg ccacggggaa ggcggctgag ttctgcccca
      721 acgcgaagat cgtccatatc gacattgatc acagcgagct ggacaaggtg aagaccgcgc
      781 atgccgggat tgtgggcgat gtccgcgcgg tgctcgaacg cctgctcccg ctgattcagg
      841 ccaatccgcg cccggaatgg ctggagcgtg tggatgagct gcgccgcgcg tttccgatgg
      901 acatgccggg cctggacgat gcccgcactc cgtatggcct catcgcagag accgcccggc
      961 atcttgacga ctcggccatc gtgaccaccg atgtcggtca gcaccagatg tgggcggccc
     1021 aggcgtatcc gctgcgacgt ccccggcaat ggctcacgtc aggcgggctt ggcaccatgg
     1081 ggtttggcct cccagcagcc attggcgcgg cgctggcatg cccggagcgc gacgtcgtgt
     1141 gcatcagcgg cgacgggagc ctgctgatga acatccagga actcgtgacg gccgccgaag
     1201 aaaatgcgaa cgtcaagatc atcgtcatga acaacaactc gctggggctg gttcatcaac
     1261 agcaggatct gttctacggg aagcgcattt ttgcgtcgga tttcgtcgtg gcggttgatt
     1321 tctgcatgat cgcccggggg tttggcatgc ggacggccga tctgggtgcc actgacgatc
     1381 ccgcgggaat gctcgcagaa gcgatgacaa cgcccgggcc gtgcctcatc aacgcgccta
     1441 tcgacgtgaa cgagaaagtc tatcccatgg ttccaccggg cgcggccaac cgcactatgg
     1501 tggcgtacga gacggttgca ggatgaggtg aggtgcagac gagactcgaa ggactcgctt
     1561 gttacgccgg atgcagtttg cgcaccggcg gcccgtaagg agacaaacca tgagtattgc
     1621 aacagaatcc gctgcaattt ccgcgttgcc acgcacggtt ctcgaactga cggtcaacaa
     1681 ccatccgggc gtgatgtcgc atgtgtgtgg cctgtttgcg cggcgggcct ataacgttga
     1741 aggcattttg tgcatgccga tcggggatga ggcgagcagc cgcatctggc tgctggtgaa
     1801 tgaagatgcg cgcctcgacc agatgatcaa gcaggccgaa aaactgcacg atgttcgtgc
     1861 cgtgcgccgc catccagcaa cgcgcgaggt gttcgaacaa ctggaagcgt ttttccggga
     1921 ataacatccg aggtcgtttg gctgtccggg cgtttccagt ctcaaacgaa ggcctgccgc
     1981 cgtgccgggg ccggtgcgcc cgtgtggcaa gcgcctgcga acggaacatt tgtgggtctg
     2041 gcattgctct tttgccccgt catgcgcggg cttgttattc tacccggagc cggggctcgg
     2101 cggccgggga atgacacggc cggtgcgatg gccccgtttt tttatggaaa ggggcgattc
     2161 atgagggtgg gcatcatcgg ttttgcgcgc tcgggcaaga cgaccgtgtt caattcgttg
     2221 acgggggcgc gtgccgaggt gggcgcgttt gggagccggg aagcgaatct ggccgtgttg
     2281 aaggtgcccg acgagcgcgt ggaccggctg gcggagatct acgagccgaa aaaggtcact
     2341 tacgctgagt tccagtttgt ggatattgcg ccggacgagg cggcgggcga agaaaaggcc
     2401 ttgagcagcg cggcgctgac ggcgctgcgg caggtcgaag cgcttgtgca cgtggtacgg
     2461 gcttttaccg gcgaaaccgt cgtgcatcca ctgggaagag tggacgcggc acgggactgc
     2521 cgtgcgctcg aagaagagat gcagctcgac gatctgatca tcattgagcg gcggctcgag
     2581 cggatggaga aggagaacag gaaagaccgg cagtacgagt tgctggcgcg gtgcaaagcg
     2641 catattgaag cggggaagcc gctgcgaacg ctcgagttga gtgcgctgga agagaaggat
     2701 attgcgggat tctgtttttt gtcgcgcaaa ccgctcttgc tgctcggcaa ctacggggat
     2761 gagcgtattg gcgatgcgga cccggcaaaa ttgcatgccc tggccgcgga aaccggtcag
     2821 accctcattg agctgtgcgg ggcgatggag atggaggtga gccagcttcc tgaggatgaa
     2881 cgggccgcgt tccgggaaga attgggcctg ggcgagggtt cgcggacccg gtttctgaag
     2941 agcgcctatg ccatgctcgg gttgatcagc ttcttgacgg cggggaagcc ggaggtccgc
     3001 gcgtggacca ttcggaaagg gacgcgcgcc caggatgcgg ccggtgtgat ccacagcgac
     3061 ctggcgcggg ggttcatccg cgccgaggtg acgcactacg acgacttcgt gaacgccggt
     3121 tcgatggcaa aggcgaagga agcgggcctc ctgcgccttg aaggcaagga ctacatcgtc
     3181 caggacggcg acatcatgct gatccggttt aatgtgtagc cgcggcccgt ctgcacagtg
     3241 aattgccggc gccggtatcg gagcagctgc gtgagggatg gcgcggccgc gttgtccctg
     3301 aggggagata acggtagaat gggccagggt gcggattgcc ggcacgtgcc tgcctttttc
     3361 gagatacgac ggttgcgggg atacagcacg agaacgcggc ccgcaggaga tggaaggctg
     3421 aggaggagac ccatgaatgt tggagtaatc ggatgcaaca cgatgggcct gatgcactgc
     3481 gagatggcgg tcaatgcagg cctgaaggtc gttgcctgcg gcgacatcag ccgcgacaac
     3541 gcccggaagg cagcccgcaa gttcggcgcg gaggccgtcg gggattgtgt tgagttgtgc
     3601 agccgcgatg atgtggatgt ggtggcgata tgtacgccaa cacccaccca cacggaatat
     3661 atcaaggccg cggccgccgc gggcaagcac gtgttctgcg agaaaccgct ggggcggacg
     3721 ctggagaaga tcgacgaggc gctggccgcg gtcgaacagg caggcgtgaa actctatgtg
     3781 ggccacgtgg tgcgcttttt tcaggaattt gaggccatga aggcccgcat cgactcgggc
     3841 cagattggca aagtggggtt tgtgaaaacc taccggggcg ggatctttcc gggcggcaag
     3901 ctgggctggt tcagcgactg gagcatgagc ggcggcgtca ccctggacac catcgtgcat
     3961 gacatggact ggctgcgcta cgtatttggc gatccggagc gtattttcgc gcagaatctg
     4021 aaacgggtgt tcagcgaacg gcttgactac gccctggtca cgatgcgcat gaaaagcggc
     4081 gtgatctgca acgtcgtggg cacctgggcg cacccgagcg ggttccgggt caaggtggat
     4141 atctgcggcg agcgcggtat ggtgacgttc gacagcagcg aggcgccggt ttccgcgcag
     4201 ttgcgccagg gagcaggttc gggaccgagc acgatcgtgc cggcgagccc ggtgctggtg
     4261 agtccgtatc aactcgaatg ggaagacttc gccggttggc tggccggcac gcacgagccc
     4321 cgggtcaccc ccgaagacgg gcgctgggcg gtgcggatgg cgcttggggc gctggaatcc
     4381 gcagaaaccg gggaaccggt cgagttcaac tgaatgcgga gcgccgtagt gagagaagga
     4441 gacgcctgat ggccaagatc ggagtgatga gtttcgcgca catgcacgcg caaagttacg
     4501 cggcctgcct caacgagctg cccgaggctg agctggttgc gatatgggat gatgatgcgg
     4561 ctcgcgggca gcagcaggcc gaggcgttcc gcacggactt tgtcccggac ctggacgcct
     4621 ttctcgccag ggatatcgac ggcgtcatca tcgcatcgga aaacgtcaaa caccgcgaaa
     4681 tggtcgagcg ggcggcggcg gccggaaaat ggatcctctg cgaaaaaccg ctggcgccaa
     4741 cggttgaaga ctgccaggcc atgatcgagg cctgccggaa ggcgggcgta ggcctcggga
     4801 cggcgtttcc ctgccgctac attccatcgg tgatggccgt gaaagacgag cttgataaag
     4861 gggtctacgg cgaaatctat gccgcatcct gtaccaacaa cggacaattt ccgggaggct
     4921 ggttcgctca acaggccctg tcgggcggcg gggccgtcat ggaccatacg gtgcatgttg
     4981 ccgacctgct gcgctggatg ctgaaccggg aatttacccg ggtgtattgc gagaacggcc
     5041 ggcttctgcg gccggagatc gatgtagacg acgtcggcag cctgcacctg gaaatggagg
     5101 gcggcgtgat cgtgtcgcac atcgcgagct ggaaccgcgc cgcgagcttt ccgacgtggg
     5161 gcgacatcac cctggagctg atcggcgaga agggcgtggt gtccgtggat gcgttcaacc
     5221 agaagctgaa cgtctatgac gacaccgcga tgcgcgccgc gtgggattac tggggcggga
     5281 gccctgagct tgggctggtg aaggcctttg gagaggcggt cgacgcaaag c
//
LOCUS       DAOPBQ010000227         5313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13210_length_5313_cov_5.321795, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000227 DAOPBQ010000000
VERSION     DAOPBQ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13210_length_5313_cov_5.321795"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PKW60_08420"
     CDS             <1..1091
                     /locus_tag="PKW60_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006454234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPJ99493.1"
                     /translation="VIKADAAIQYLEARGHSEAVAYLNRIEKPKVSVSFKGSSTESVG
                     NRSEFGKIVCDILAQMTPEERQQKVLVVDSDLEGSCGLDQIHKNYPEVFVPGGVMERG
                     NVSAAAGFGFDAGQQGIFATFSAFLEMVLSEVTMARLNHANLLCHFSHAGVDEIADNT
                     CHFGINNFLADSALPEGDTTRLYFPADGHQFRKALETIFHDPGIRFIFSNRSKLPYIL
                     REDGSHFFGDGYHFERGKDDLIREGSAGYVVAYGELLYRALDAVERARENGINVGLIN
                     KATLNVIDEAMLEKAGQAPFVLVAEGQNVKTGLGSRYGTWLLQRGLTPKYAHLGAVRP
                     GRGGIAEQMPYQGLASDDILEKIRQLHG"
     gene            1203..1721
                     /locus_tag="PKW60_08425"
     CDS             1203..1721
                     /locus_tag="PKW60_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPJ99494.1"
                     /translation="MSSDIRPIEWNFCPTCGAALTLANDGERPRPHCRACGRFFYSNP
                     VPAACCLVARGDTLLLARRAVEPCIGEWALPGGFVELGETTEEAALRELEEETGLRGA
                     RPRLIGASTQPSRLNGAVVVMGYVIEDWEGEPSARSDVLEAKFFAPGERPPLAFLAHQ
                     ALVAIYDALESR"
     gene            1918..3996
                     /locus_tag="PKW60_08430"
     CDS             1918..3996
                     /locus_tag="PKW60_08430"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPJ99495.1"
                     /translation="MKRLGIAVLCMAVIGCQTIPPEPEVPVEPSVPITDTAPPFRLED
                     VPPPDMSALAGRRICIDPGHGGRWPGAVAPSNRLRESDVNLQVALALRDLLRAAGAEV
                     VLTRETDTALDASRLSQDLSARPGLANRVNAEAFISVHHNAHIIEGSDKNDLEVYYKL
                     GDAGPSLDLAQCMMREMALRVRQDTEPKRLLPGNYKVLREARVPAVLMETSYMTNARN
                     AEFMATRYGVEKEAAGLASGLAAYFAMAPPSVLGQELVEHADGRTQALIYTLGGGLPL
                     ERDTLELRIDGQLVDGIAVESGNTLTWYLDRILGNGPREIAFFARNTRGASLSSETTV
                     PVARPPAFITVHQYPDAPVRASGIEYLFEVYVTDRFGLPVADGTPVTVNENLLKVPTK
                     SGTARCYFLEDTLPAQLTMRAGEITEQVAPRFGSEAFRTVRLHDVRTSQPVAGVTAFS
                     EGRVLGTSNSEGWLPLPAATRGLSVRKNGFDEVTVALNGGHAVVGLIPTAGGTLHGKT
                     IMLDAAYGGSNAGAVGPTGMRASDAALDVVRRVAMRLRQRGATVVLAREDDSDLSPLE
                     RVRLSDAAGASVHVVVAFGADSATAKVVDQRGLLVGKNVQFAAHYPGSSGGERLAQTV
                     ASALGGLPVTTSVTYLLQQTACPAVWVQPQSIEDARAEHLLQDPETRRSFAEALAGAI
                     EKHFRGKNEE"
     gene            4343..5209
                     /locus_tag="PKW60_08435"
     CDS             4343..5209
                     /locus_tag="PKW60_08435"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPJ99496.1"
                     /translation="MYSHRVFPRLIELHDLPALPQVVTQLLAAVEGGHSSTDEMTALL
                     ETDQAISACVLDIANSRYYGAPGGMDSIRRAVAVLGFDAVYQLALSASVFKAFFSQHQ
                     FSLEPRDFWMHAFGTAKAAQLLCHGPVTLPSPEACFTAGLLHDIGRYLLALVLRNEYR
                     AVLSQAGEQKMLLHECEKKRLGVNHPEVGRWLTAKWRFPSLIVEIIGNIHRPATAKGK
                     WATEIAVVQLAATLARRAGFGDAGDWKAPAISGLQLEFLRLTPRQVEDLLKILGEMRT
                     TTRDLLKHLKAG"
BASE COUNT         1003 a         1665 c         1650 g          995 t
ORIGIN      
        1 acgtgatcaa ggctgacgcc gccatccagt atctcgaagc gcgcggccac agcgaagccg
       61 tcgcctatct caaccgcatc gaaaagccca aggtctcggt gagcttcaaa ggcagttcca
      121 ccgaatcggt gggcaaccgg agcgaattcg gcaagatcgt ctgcgatatc ctggcccaaa
      181 tgacgcccga agaacgccag cagaaggtgc tggtggtcga ctccgacctg gaaggttcat
      241 gcggcctgga tcagatccac aagaattacc cggaggtttt tgtgccgggg ggcgtcatgg
      301 agcgcggcaa cgtatccgcc gccgccggtt tcggcttcga tgccggccaa caggggatat
      361 tcgcgacctt ctcggcattc ctcgagatgg tgctctccga agtgaccatg gcccggctca
      421 atcatgccaa cctgctgtgc catttctcgc acgcgggcgt cgacgagatc gccgataaca
      481 cctgccactt cggcatcaac aatttcctgg ccgacagcgc cctgcccgag ggcgacacca
      541 cccgtctcta ttttccggcg gacggacacc agttccgcaa ggcgctcgag accattttcc
      601 atgacccggg catccgcttc atcttctcca accgctcgaa actgccgtat atcctccggg
      661 aagacggctc gcacttcttc ggcgacggct accatttcga gcgcggcaaa gacgacctca
      721 tccgtgaggg cagcgccggt tacgtggtgg cctacgggga actcctctac cgcgccctcg
      781 atgctgtcga gcgcgcgaga gagaacggga tcaacgtcgg cttgatcaac aaggccacgc
      841 tcaacgtgat cgacgaggcc atgctggaga aagcgggcca ggcgccgttt gtgctggtcg
      901 cagaaggcca gaacgtgaag accggcctgg gctcgcgcta tggcacctgg ctgctccaac
      961 gcggccttac tccgaaatac gcgcatctcg gcgcggtacg gcccgggcgc ggcggcattg
     1021 cggaacagat gccgtatcag ggcctggcct ccgacgacat cctcgagaag atccgccagc
     1081 tccacggctg aaaatccatt tgaaatgcgt ttcggggcgg tcgccaacgc ggccgccctg
     1141 ttcattttgg attgcgttgc ggttgaatag acaaacccgc ggcgggttgc tacagtgacg
     1201 ccatgagctc tgacatccgg ccgatcgaat ggaatttctg ccccacctgc ggcgccgcgc
     1261 tgaccctggc caatgacggc gagcggccgc gtccccactg ccgcgcctgc ggccgtttct
     1321 tttacagcaa ccccgtgccg gccgcatgct gtctggtcgc gcgtggggac acccttctgc
     1381 tggcgcggcg ggccgtcgaa ccgtgcatcg gcgaatgggc attgccggga gggtttgtcg
     1441 agctgggcga aaccactgaa gaagccgcct tgcgcgagct tgaggaggaa accgggctcc
     1501 gcggcgcccg gccccggctc atcggcgcca gcacccagcc cagcaggctc aacggcgccg
     1561 tggtggtcat gggctacgtg atcgaggact gggaaggcga gccctccgcc cgatccgacg
     1621 tgctcgaggc gaaatttttc gcgcctggcg agcgtccccc tctcgcgttt ctcgcccacc
     1681 aggcgctcgt cgcgatctac gacgccctgg aatcacgctg agtatttccc gcccgcgagc
     1741 cgcttgcatg tgatacaaag ttcagggtag cgtatgcctc gtgttctggc agcgaagttc
     1801 gcgttcgcat cgaacggtat gcgtgctgta tatgctccac tgccgcgtgg ttgttttctc
     1861 agcggtttgg attcgtcctc tgcgccgatg taatccgtaa gtcacgaagg aaagtcaatg
     1921 aaacgtctcg gtattgctgt cctgtgcatg gccgttatcg gctgccagac gatccccccg
     1981 gagcctgaag tcccggtaga gccttccgta ccgattaccg ataccgcgcc gccgttccgt
     2041 ctggaggatg tgccgccgcc cgacatgtca gcactggcag gccgccggat ctgcatcgac
     2101 ccgggccatg gcggacgctg gccgggggca gtcgcgccat ctaaccgctt gcgcgaaagc
     2161 gacgtcaacc ttcaggtggc gctggcgctg cgcgatttgc ttcgcgccgc aggcgcggag
     2221 gtagtcctaa cgcgtgagac cgataccgcg ctggacgcca gccgcctgtc ccaggacctg
     2281 tccgcacgcc cgggcctggc caaccgggtg aatgcggagg ctttcatcag cgttcatcac
     2341 aacgcccata ttatcgaggg ctccgacaaa aatgacctcg aggtgtacta caagctcgga
     2401 gacgccggcc ccagtctgga tctggcccag tgcatgatgc gcgagatggc cctgcgcgtg
     2461 cgtcaggata ccgagcccaa acgcctgctt ccgggcaact acaaagtcct ccgcgaagcc
     2521 cgcgtgcctg ccgtgctcat ggagacgtcc tacatgacca atgcccgcaa cgcggagttc
     2581 atggccacgc gctacggcgt ggagaaggaa gcggcgggcc tggcttcggg tctggccgcg
     2641 tattttgcca tggcgcctcc gagcgtgctc ggccaggagc ttgtcgaaca cgccgacggc
     2701 cggacccagg ccctcattta tacactgggc ggcggactcc cgctcgaacg ggatacgctc
     2761 gagctgcgca tcgacgggca gctcgtcgac gggatcgccg ttgaaagcgg aaacaccctg
     2821 acctggtacc ttgaccggat tctcggcaac gggccacgcg agatcgcgtt tttcgcccgc
     2881 aacacgaggg gagcgtcgct ttccagcgag accaccgttc cagtagcacg cccgcccgcg
     2941 ttcatcaccg tgcaccaata ccctgacgcg ccggtacgcg ccagcggcat cgaatacctg
     3001 ttcgaagtct acgtgaccga tcgcttcggc ctgccggtgg ccgacggcac tcccgtgacg
     3061 gtgaacgaaa atcttttgaa ggttccgaca aagtcgggca cagcgcgctg ctactttctc
     3121 gaagatacgc tgcccgcgca attgaccatg cgggcgggag agatcacgga acaggttgcg
     3181 ccccggttcg ggtccgaagc ctttcgcacg gtgcgattgc acgatgtccg gacaagccag
     3241 ccggtggccg gtgtcacggc gttttcggaa gggcgcgtcc tgggtacgag caacagcgag
     3301 ggctggctgc ctctgcccgc cgccacgcga ggcctttccg tgcgcaagaa cggcttcgat
     3361 gaagtcaccg tcgctctgaa cggcgggcac gctgtggtgg gtctgatccc caccgccggc
     3421 ggcacacttc atggcaaaac catcatgctc gacgcggcct acggcggcag caacgccggc
     3481 gccgtgggtc ccaccgggat gcgcgccagc gatgccgcgc tcgatgttgt gcggcgcgtg
     3541 gctatgcgcc tgcgccagcg cggggcgacc gtcgttctgg cccgcgaaga cgacagcgat
     3601 ctgtcccccc tggaacgggt tcgcctgtcg gatgctgcgg gcgccagcgt tcacgtggtg
     3661 gtggcgtttg gggcggattc agccacggca aaagtggttg atcagcgcgg cctgctggtc
     3721 ggaaaaaacg tgcagttcgc cgcgcattac cccggcagtt cgggtggcga gcggctggca
     3781 cagaccgtcg caagcgccct cggcggatta cccgtgacga catcggtcac gtacctgttg
     3841 cagcagacag cctgcccggc ggtatgggtg cagccgcaat ccatcgaaga tgcgcgggca
     3901 gaacacctcc ttcaggaccc ggaaacccgc cgcagcttcg cggaggcgct cgccggagct
     3961 atcgagaagc attttcgcgg gaaaaatgag gaataatcgc gaaaaaggcc aacttggccg
     4021 ggtttttctg gcttttccat ttgcgagagg gtaaagtctt atatataaag aatttaatcc
     4081 gcaatgctgg ttttcatgat aagctttaca tataattctg tctgaaccga ggaacgctcg
     4141 cgccgctgag atgttcctct catcaaaaat cagccgtata tctgaccagg atagggcagg
     4201 tttctggatt tttccgatca tgcgggggtg ggatttggta gaataccgtc gtaagaccga
     4261 cgcgaatggg cgccggacag gtgtaatacc ctataatcat gccagccatc cggacaccgc
     4321 tgcggggacg gaggctacgt gaatgtacag ccatcgtgtc ttccccaggc tcattgagct
     4381 ccatgatctg ccagccttgc ctcaggtggt aacccagttg cttgcggctg tcgaaggcgg
     4441 gcattcctcg acggacgaga tgacggcgct gctcgaaaca gatcaggcca tctcggcctg
     4501 cgtgctggat atcgccaact cgaggtatta cggagcaccc ggcggaatgg actccatccg
     4561 ccgggccgtt gcggtgctgg ggttcgatgc cgtgtaccag cttgccctgt cggcctccgt
     4621 gttcaaggca ttcttcagcc agcaccagtt ttcgctggaa ccgcgcgatt tctggatgca
     4681 cgccttcggc accgccaaag cggcccagct cttgtgccac gggcccgtga ctctgccgtc
     4741 gccggaagcg tgtttcacgg caggcctgct gcatgatatt gggcggtacc tgctggcgct
     4801 tgtgcttcgc aacgaatacc gtgccgtgct gagccaggcc ggcgaacaaa agatgctcct
     4861 tcacgaatgt gaaaagaagc gattaggcgt aaatcacccg gaagtggggc gctggcttac
     4921 cgcgaaatgg cgatttccat cgctgatcgt cgagatcatc ggcaatatcc atcgtccagc
     4981 cacagccaaa gggaaatggg ccacagaaat tgcggtggtg cagcttgcgg cgaccctggc
     5041 gcggcgggcc ggtttcggcg acgccgggga ttggaaagca ccggcaataa gcggcctcca
     5101 gcttgaattc ttgaggctga ccccacgcca ggttgaagat ctgctgaaaa ttctggggga
     5161 aatgcgcacc actacccgcg acttactcaa acacctgaaa gcagggtaaa ccgccggggt
     5221 tcatgcgggc cgccgagggt tcgtgatcaa tccggctctg ttcgtaaaaa agggcggggc
     5281 cgtcagggcg cagaccagat caggtcgccg tgt
//
LOCUS       DAOPBQ010000228         5290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13272_length_5290_cov_4.634766, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000228 DAOPBQ010000000
VERSION     DAOPBQ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13272_length_5290_cov_4.634766"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PKW60_08440"
     CDS             <1..1317
                     /locus_tag="PKW60_08440"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HPJ99497.1"
                     /translation="GLGAEAEEIIPQVTPETVQQLEYRLINIIFKLTREKKPVVALVA
                     PKESFNIDPQMRQIMMQMGQAIPEQRDPYDVLQAYLESEKYEVRRVELSKDSPLPEEY
                     DTLAVINPRQLNERQRWEIGRALASGKSVILAVQNYLWDYRTSSRGITMSRQDENPQI
                     NELLAKYGLGVDDDLLMDINSFPLRIRSNDPLQNLLGGGTPITSPMHVEVTSEQMDSE
                     TSITSRLGFITYLWGTALTIDQEELKKHGLEARVIMHTSNKAWTASPNAQLSQELFEP
                     PASGDQYPLMALVTGQFPDAYAGQERPNWPPPQPQPGMPPMPPPDEDEGPAPPLTAAP
                     GKLILLGASQMFRSDFLQQSNADLFLNSIDALTLGDDLVNVRSRKPVDRTISKPSASK
                     VNAWKFVNYVLVSLVIIAAGTIVSVVRRQSRNAYTVSFMNNSPPAE"
     gene            1377..3410
                     /locus_tag="PKW60_08445"
     CDS             1377..3410
                     /locus_tag="PKW60_08445"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPJ99498.1"
                     /translation="MRKWKNVVPLAVILAILAALVVLKQIQNRPVAIEERLQAKVTPL
                     VPETVTKGAVTRLDLYSGAKPDEKVTIERDAADPNVWRVASHFNAPAQKGKIDTFLDK
                     LADLRGEYRAGGVTGDDLKDFGLADDSAFHVLAYTGDPEAAAVHILNGKAPKSGQVFV
                     RTADGNTVYVADVNFRNEAMIWSGEADAAPEPGPWENKDIVKIDPEQITRVAVTMPDK
                     QLVFEKREKPVEKKEGGDAAPEAAEPPAPVYEWALASGGVAGQTFKQAGLDALVRAFN
                     PLSATEIVDPEKLEEWNLASPGFKCVLTLKDNKEVVLEGGLPESSADGYLRVVGTPEP
                     VVYKLSSYNFGKVFPKGSDFFDLQTLALDKETLTRIEMDLPGIRAVLAKEGAGWTVIE
                     PAAELEADTTTISTLSGALAAWKASDYTDSAALGEPVRKITFTTSDGASHVLEIGGPS
                     RSIDGSYARLDGGPVVAMTKGDVGKILVEPRNLYRRALLEKTADDIQSVTITPAGGEP
                     LVLTRNESGAWMLGDAEADPETAGDLAETIADIQAEDILFGKTSADFVPRITVDVTVK
                     TGEAYRLVLGAETDAGTEFLMDGKATVFVMAKEDAAALTPSADALKKPEPAAAAADTP
                     EESAPAADVSAEDAPAEEVSAVEVSEAVAPAGEAPAPDVAVEEAPAADAEAQQ"
     gene            3462..4568
                     /locus_tag="PKW60_08450"
     CDS             3462..4568
                     /locus_tag="PKW60_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPJ99499.1"
                     /translation="MHRALVLCSVMAWIAAAPVSAQDGVDQSRRNALVQAIEKAAPAV
                     VSINVLETQADIPPLFRDFYEFFDLQPPRRRVENRRIDSVGSGFIIDREGHILTNYHV
                     VEDASGIASVTLPDGRDLTASIVGVDPRTDLALLKAEGTDLPFTVLGESDDLYIGEWV
                     IAIGNPFGPLISDPQPTVSVGVVSAKNRRLSPSVGKGARLYQNMIQTDAAINPGNSGG
                     PLVNARGQAIGVNTMIFSQSGGSVGLGFAIPISRARRVVAELQRFGRRRDPWAGFKVE
                     DVAGLRPGVARQHGITSDHGCLVVNILRDCPAYAAGLRPGDIIVGIAGQEVMRSSDID
                     FAIWGHFVGDAISIDIDRQGERQSLTFVLEELSR"
     gene            complement(4585..>5290)
                     /locus_tag="PKW60_08455"
     CDS             complement(4585..>5290)
                     /locus_tag="PKW60_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HPJ99500.1"
                     /translation="ALYMQGHAERDRRTLLCDAPSLAMHESQSRMWENVIGRSLPFWR
                     HYLPVMRDFFPGQLDTVTPEEVYAAVNLAKPSLIRVDADECTYNLHIIIRFELEVALI
                     EGSLDPSSVPEAWNSKVREYLGLEVPDDARGCLQDIHWAHGAFGYFPTYALGNLYAAQ
                     LFNAMLEQVPGLWESVEQGDFRPMLAWLRTNVHVHGRRKLARELLRDATGADLSPEPY
                     IAYLNHKFGALYSLGI"
BASE COUNT         1095 a         1655 c         1652 g          888 t
ORIGIN      
        1 ggcctgggcg ccgaagctga ggagatcatc cctcaagtga cccccgagac ggtacagcag
       61 ctcgagtacc ggctgatcaa catcattttc aagcttacgc gcgagaaaaa gccagtcgtg
      121 gccctggtcg cccccaaaga atcgttcaat atcgatcccc agatgcggca gatcatgatg
      181 cagatgggcc aggcgatccc cgaacagcgc gacccctatg acgtgcttca ggcctatctg
      241 gaatcggaaa aatacgaagt gcgccgcgtc gagttgagca aagacagtcc cctgcccgag
      301 gaatacgaca cgctggccgt catcaacccg cggcaactga acgagcgaca gcgctgggaa
      361 attggccgcg ccctggcctc cggcaaatcc gtcatcctgg ccgtgcagaa ttacctgtgg
      421 gattaccgca ccagcagccg cggcatcacg atgagccgcc aggacgaaaa cccccagatc
      481 aatgagctcc tcgcgaaata tgggctggga gtggatgatg accttttgat ggacatcaac
      541 agcttcccgc ttcgcatccg cagcaacgat ccgctccaga acctcctggg cggcggcacg
      601 ccgatcacca gcccgatgca cgtggaggtc accagcgaac agatggacag cgagacctcg
      661 atcacgagcc ggctcgggtt catcacgtac ctgtggggta cggcgctcac gatcgaccag
      721 gaagagctga aaaagcacgg tctcgaagcg cgtgtcatca tgcacacgag caacaaggcg
      781 tggacggcgt cgcccaacgc ccagttgagc caggaactgt tcgagccgcc ggccagcggc
      841 gatcagtatc cgctcatggc cctggtcacc ggccagtttc ccgatgccta cgcgggccag
      901 gagcggccaa actggccgcc gccccagccg cagccgggca tgccgcccat gccgccgccg
      961 gatgaggacg aagggcccgc gccgccgttg accgcggccc cgggcaagct catcctgctg
     1021 ggagcgtctc agatgtttcg cagcgatttt ctgcagcaat ccaacgcgga cctgttcctc
     1081 aacagcattg acgcgctgac cctgggtgac gacctggtga acgtgcgcag ccggaaaccg
     1141 gtggaccgca cgatctcgaa accgagcgcg agcaaggtca acgcgtggaa attcgtcaac
     1201 tacgttctgg tgagcctggt tatcatcgcg gcaggcacga tcgtaagcgt ggtgcgccgg
     1261 caatcgcgca acgcgtacac cgtatctttc atgaacaaca gcccgccagc cgaatgacgg
     1321 cggcgcaagc cgaagatata ctgggaacgt cccgataagg attgtggaga ggtaccatga
     1381 gaaagtggaa gaatgtggtt cccctggcgg tgatcctggc cattctggcg gccctggttg
     1441 tgctgaagca gatccagaac cgcccggtag cgatcgaaga acggcttcag gccaaagtca
     1501 cgccactcgt acccgaaacc gtgacgaagg gcgcagtaac tcgcctcgac ctctattcgg
     1561 gcgctaaacc ggatgagaaa gtcacgatcg agcgcgatgc cgccgatccg aatgtgtggc
     1621 gtgtcgccag ccacttcaac gcgccagccc aaaaaggcaa gatcgacacg ttcctcgaca
     1681 aattggccga tctgcgggga gaataccgcg ctggcggcgt gaccggcgat gatttgaagg
     1741 atttcggtct ggctgacgat tccgcgttcc acgtgctcgc gtataccggc gatccggaag
     1801 cggccgccgt acatatcctg aacggcaagg cgccgaaatc cggacaggtc tttgtgcgca
     1861 ccgccgatgg aaacaccgtc tacgtggccg atgtcaattt ccgcaacgaa gccatgatct
     1921 ggagcggcga agccgatgcc gcgcctgaac ccggcccctg ggaaaacaag gacatcgtaa
     1981 aaatcgatcc ggaacaaatc acccgggtcg cggtcaccat gccggacaaa cagctcgtct
     2041 ttgaaaagcg tgaaaaaccc gtggagaaga aagaaggcgg ggacgccgcg ccagaggccg
     2101 cggaaccgcc cgcgcctgtg tacgagtggg cgctggcgtc gggcggcgtc gcagggcaga
     2161 cgttcaaaca ggcggggctc gacgcgctgg tccgcgcatt caatcccctg agcgcgaccg
     2221 agatcgtcga tccggagaag ctggaggaat ggaaccttgc gtcgcccgga ttcaaatgcg
     2281 tgctgaccct caaggacaat aaagaagttg tcttggaggg tggtcttccg gagtcgagcg
     2341 ccgatggcta tctgcgtgtc gttgggaccc cggaacccgt tgtctacaag ctgtcgtcgt
     2401 acaatttcgg caaggtattt ccgaaaggtt ccgacttctt cgatctgcaa acccttgcgc
     2461 tcgataagga aaccctgacc cggatcgaga tggatctgcc ggggatccgc gccgtgctcg
     2521 ccaaagaggg cgccggctgg accgttatcg aacctgccgc cgagctcgag gccgacacca
     2581 ccacgatttc aactctgtcc ggcgctctgg ccgcttggaa agcctccgat tacacggact
     2641 cggcggcgct cggcgaaccc gtgcgcaaaa tcaccttcac cacaagtgac ggcgcgtcac
     2701 acgtcctgga gattggcgga ccgtcgagat cgattgatgg cagctacgcc cgcctggacg
     2761 gaggccccgt cgtcgccatg accaaaggcg atgtgggcaa gatcctggtc gaacccagga
     2821 acctctaccg gcgtgcactg ctcgagaaga ccgccgatga catccagtct gtgaccatta
     2881 ccccggccgg cggcgagcct ctcgtcctca cccgcaacga aagcggcgcg tggatgctcg
     2941 gcgatgccga agcggatccg gaaaccgcag gggacctcgc cgagaccatc gcggacattc
     3001 aggccgaaga catcctcttc gggaaaacat ccgccgactt tgtgccccgg atcaccgtcg
     3061 acgtcaccgt caagacggga gaggcgtacc ggctcgtcct gggagccgaa accgacgccg
     3121 gaaccgaatt tctgatggat ggcaaggcaa cggtcttcgt gatggccaaa gaagatgccg
     3181 ccgccctgac gccatcggcg gatgccctga aaaaacctga gcccgcggcc gcggcggcgg
     3241 atactccgga ggaaagcgct ccagcggcag atgtctccgc ggaagatgcg ccggccgaag
     3301 aagtctccgc agtagaagtg tctgaggcag ttgcgcctgc cggggaagcc cctgcaccgg
     3361 atgttgctgt agaagaagcc cccgcggcgg atgccgaagc acaacaatag tcacggtgaa
     3421 tgacagagct gcgccgctgg cgcagtagag gagcggtcgc catgcaccgc gcgctggtct
     3481 tgtgcagtgt gatggcgtgg attgccgccg cgccggtttc tgcacaggat ggcgtggatc
     3541 aatcgcggcg aaacgccctg gttcaggcca tcgagaaagc cgcgcccgcc gtcgtgtcca
     3601 tcaatgtgct ggagacgcaa gcggatatac ctccgctgtt ccgcgacttc tatgagttct
     3661 ttgacttgca gcccccgcgg cggcgtgttg agaaccggcg catcgactcc gtgggcagcg
     3721 gattcatcat tgaccgggaa ggccacatcc tcaccaacta ccacgtggtc gaagatgcca
     3781 gcgggattgc ttccgtgacc ttgccggacg ggcgtgacct cacggcgtcg attgtgggcg
     3841 tggatccgcg caccgatctc gcgcttctca aggccgaagg tacggatctg ccattcacgg
     3901 tcctgggaga atccgacgat ctgtacatcg gggagtgggt catcgcgatc ggcaacccgt
     3961 ttggaccgct catcagcgat ccccagccca ccgtcagcgt gggcgtggtg tcggcgaaga
     4021 accggcgcct gagtcccagt gtagggaagg gcgcccgcct gtatcagaac atgatccaga
     4081 ccgacgccgc gatcaacccc ggcaacagcg gcggaccact ggtgaatgcc agaggccagg
     4141 ccatcggcgt gaacaccatg atcttctccc aatccggggg cagcgtgggc ctgggattcg
     4201 ccattcccat cagccgcgcc cggcgcgtcg tggccgaact ccagcgcttc ggccggcggc
     4261 gtgatccctg ggccggattc aaagtcgagg acgtcgccgg gctccgtccc ggcgtcgcgc
     4321 gccagcacgg tatcaccagt gaccacggct gcctggtggt caacatcctg cgggattgcc
     4381 ccgcctatgc cgccgggtta cgcccgggcg atatcatcgt cggcatcgcg ggccaggagg
     4441 tgatgcgctc gagcgacatc gatttcgcca tctggggcca tttcgtgggc gacgccatct
     4501 cgattgacat cgaccgccag ggcgagcgcc aatccctcac ctttgtcctg gaagagttga
     4561 gccgctgacc ccgccgcgcc cgggtcagat tccgaggctg tacagcgcgc caaatttgtg
     4621 gttcaggtac gcgatgtacg gctcgggaga gagatccgct ccggtggcat cgcggaggag
     4681 ttcgcgtgcc agtttgcggc ggccgtgtac atgcacgttt gtgcgcagcc aggcgagcat
     4741 cggccggaaa tccccctgtt ccacgctttc ccagaggccg ggcacttgtt ccagcatcgc
     4801 attgaacaac tgggccgcat agaggttgcc gagggcatag gtaggaaaat agccgaaggc
     4861 cccatgcgcc caatggatgt cctgaaggca gccgcgggcg tcgtcgggca cttccaggcc
     4921 gagatactcc cgcaccttgc tgttccaggc ctcgggcacg ctgcttgggt ccaggctgcc
     4981 ctcgatgagc gccacttcaa gctcaaaccg gatgataatg tgcaggttgt aggtgcattc
     5041 gtcggcatcg acgcgaatca gcgacggttt tgccaggttc accgcggcat acacctcttc
     5101 cggcgtgacg gtatcgagct gcccggggaa aaaatcccgc atcaccggca aataatggcg
     5161 ccagaagggc aggctgcggc cgatcacgtt ttcccacatg cgggactgcg attcgtgcat
     5221 ggcgagggag ggcgcatcgc acaggagcgt gcggcggtcg cgttcggcat gcccctgcat
     5281 gtaaagcgca
//
LOCUS       DAOPBQ010000229         5289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13276_length_5289_cov_4.366068, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000229 DAOPBQ010000000
VERSION     DAOPBQ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13276_length_5289_cov_4.366068"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="PKW60_08460"
     CDS             <1..1371
                     /locus_tag="PKW60_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPJ99501.1"
                     /translation="LVASCFAEFAGGLYQRPDVTLAVAEGRGYLQSHGRKYDLIQVPL
                     FGSFTAAASGVYALNESYLYTVEAFRLFFQRLTPGGVYVINCWLSSPPREAIKIFATA
                     AEALRKEGIGEPGQHLVMIRSLNSATLVVSKAPLSSANVTDVRAFCRSRSFDPCYFPG
                     IQADETNRYVELSEGSVYYDAAQALVSDHAGDFYRDYLFYVRPATDTRPYFFRFFKWT
                     SLPRLIKGMGVAWAPFVEWGYVALIATVLQAGVASALCILAPLLVLGRSPAARGLKRW
                     VVVYFGLLGLGYMALEIVFMQRFMLFLAYPVYSVAVVLASFLTFSGFGSLAASRFTGS
                     AARNVAVAAAGIAAVAVVYLAALGPVFSALAGLPDAAKIALSITGLGPLAFCMGIPFP
                     LGLQSAANRSDALLPWAWGINGCASVLGATLATVSSIHLGFPAVVLLALACYGLATLA
                     IRPIVA"
     gene            complement(1391..2419)
                     /locus_tag="PKW60_08465"
     CDS             complement(1391..2419)
                     /locus_tag="PKW60_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99502.1"
                     /translation="MGSIRVGFVGLGGICRSRHVPGLRRLDGVEIVAVANRTRESSER
                     AAAQFGIPHVCDSWQELVERDDIDAVFVGTWPYLHKDVSVAALKSGKHVFCQARMARN
                     LSEARAMYEAARHSDRVAALCPVPIGLSVDATMARLIHEGALGDIRLVRVQSFSDAFA
                     RPETPLHWRNDDRLSGLNMHTLGMYAEVIHRWFGWTWTVNAIAQTFTRSRLDAAGQSV
                     RINIPDQVLFTAGMRAGFLVQYTISTAVHHGEEEIQIFGSQGSLVYNVNEDVLYGAQA
                     GEPMAAVEIRPDEARDLREWSVESDFIRAIRDGAEYHPDFFDGLKYMEVVQAVHDSAR
                     EGKTVMLD"
     gene            complement(2541..4637)
                     /locus_tag="PKW60_08470"
     CDS             complement(2541..4637)
                     /locus_tag="PKW60_08470"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPJ99503.1"
                     /translation="MRSARLTIKITPLAMAALALLCWTAQANELDEFLVRIAGPNADA
                     RAGAWKSAGPLGAPAVLPLARLAETGEPEIRVAALRAIDIITAYAGRPGAQEEQRAVA
                     QALADAVQSSEDSQLRCEFLHYLGLVASNPEVPMLARLLEDPAIGEDARIALERIPGD
                     TATRALITALRSASSETVQLRMADALARRQAPEAIPVLTELAQSSGNRRLMFACLKAL
                     GAFGVPPHRVLSRRPSFTPEERVVYVQAALAAAYRLREQGENDEAATIYQSIAAYSNE
                     PAHLSESILGLDAANAETFAAQALGYLLHPGVSTVAFRALVESQQKGLDEKLTLVCEQ
                     CEPPRKVAVLQILQLRNAESLPHLLEVARTDDALEVRVMSYLLSDETPPFEEVLTVAR
                     TGSEWIRPRALDTVFRQIATMTSEGATEAAQTACVDLLDSGLPEKEAVAAFAILEQIP
                     SEATAAYLDSLDLWQPSPGAKGLALTPAALEAAQCAYVACAAAETNPDAARERLFLAA
                     ENSLFTRVTSRALEQLVALGVDRTVLARRQGFLTDWKIIGPFPNPDGTAFERSFLDET
                     ACTGREPVVFEGKTYAWLPVETDSVPAVIGLRARLDPAEFVAAYACAELTSPEVRDVI
                     FQIGSDDGCELWINGERLHGTAGGRTMVVDQDKVDATLAEGVNRVLIKALQNRNDWQF
                     CVRVTSRDGRPLDLSR"
     gene            complement(4649..>5289)
                     /locus_tag="PKW60_08475"
     CDS             complement(4649..>5289)
                     /locus_tag="PKW60_08475"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99504.1"
                     /translation="PARWVPYNPDRVHANFRWFLDFGGGQVRDRGAHVLSLVNWFLDL
                     DEKGPVRVTAVGEPPHKGLYDCPTTMEVTWEFKDPNLTIVWKQPGNVPKGSNARFGAV
                     YYGDKGSLIVGGGDGSGLSSDASAYEPPAGSAPVYQSPGHHEDWFECIKSRKRPIMDI
                     APAHNAAKLCILANLAYRLGRPLDWDHASEQFIGDEQANRMLGNSGRGPWHL"
BASE COUNT          914 a         1656 c         1694 g         1025 t
ORIGIN      
        1 ttggtggcat cgtgcttcgc ggaatttgcg ggaggtctgt accagcggcc ggacgtcacc
       61 ctcgccgtcg cggagggccg gggctacctc cagtctcacg gccgaaaata tgacctgatt
      121 caagtgccgc tcttcgggtc ctttacggcg gccgcctccg gggtctatgc gctcaatgag
      181 agctacctgt acacggtgga ggcgttcagg ctgttttttc agcgtctgac gcccggcgga
      241 gtgtatgtga tcaactgctg gctgtcctct cccccgcgcg aagccatcaa aatctttgca
      301 accgcggccg aggcgctgcg caaagagggc atcggcgaac ccggccagca tctcgtgatg
      361 atccggtcgc tcaacagcgc gacgctggtg gtgtcgaaag cacctttgag cagcgcgaac
      421 gtaacggacg tgcgggcttt ctgccggtcg cggagctttg atccctgtta ttttcccggc
      481 attcaggcgg atgaaaccaa ccgctacgtc gaactatcgg agggaagtgt ctattacgac
      541 gcagcgcagg cgctcgtatc cgaccacgcc ggggattttt accgggacta cttattttat
      601 gtgcgtccgg ccaccgatac gcgcccgtat ttcttccggt tcttcaaatg gacgtcgctg
      661 ccccgcctga taaaaggaat gggcgttgcc tgggcgccgt tcgttgagtg gggctacgtg
      721 gccctcatcg cgaccgtcct tcaggcaggc gttgcgagcg cgctctgtat tctcgcgccg
      781 ctgctcgtgc tcggccggtc accggcggcg cgaggactca aacgctgggt ggtggtctat
      841 tttgggctgc tgggcctggg atacatggcc ctcgagatcg tgttcatgca gcggttcatg
      901 ctatttctcg catatccggt gtattcggtc gcagtcgtgc tggcatcatt tctcacgttt
      961 tcaggatttg gcagccttgc cgcgagccgc tttacggggt ctgcggcgcg aaacgtggcc
     1021 gttgcggcag cgggcatcgc cgcggtagcg gtggtctacc tcgccgcgct tgggccggtg
     1081 ttcagcgcgt tggccgggct tccggatgcc gccaaaatcg ccctgagcat cacggggttg
     1141 gggccactgg cgttctgcat gggaattccg tttcctctgg gccttcaaag tgcggccaac
     1201 cggtcggatg ccttgttgcc gtgggcctgg gggatcaatg gctgcgcgtc agttctgggc
     1261 gccacgctgg ccaccgtaag ctcgatacac ctgggatttc cggccgtggt gctgctggcc
     1321 ctggcgtgct acggactggc cacgctggcg atccggccga ttgtggcgtg agtgtgcgcc
     1381 ggggcggcgg ctaatcgagc atcacggttt tgccttcgcg ggccgaatca tggacggcct
     1441 gcaccacttc catatatttg agaccgtcga agaaatccgg gtgatactcc gcgccatcac
     1501 gaatggcgcg aatgaaatcg gattcgacgg accattcgcg caggtcgcgc gcctcatccg
     1561 gacgaatctc cacggctgcc atcggttcac cggcctgcgc gccatacagg acgtcctcgt
     1621 tgacgttgta tacgagggag ccctgagagc cgaatatctg gatttcttcc tcgccgtgat
     1681 gcacggcggt gctgatggtg tactgcacca gaaaaccggc gcgcatgccc gcggtaaaca
     1741 ggacctgatc ggggatattg atgcgcacgg actggcccgc cgcatcaaga cggctccggg
     1801 taaacgtctg ggcgatcgca ttgacggtcc aggtccagcc gaaccaccgg tggatgacct
     1861 cggcgtacat gccgagggta tgcatgttca gacccgacag gcgatcgtcg ttgcgccagt
     1921 gcaggggagt ctcggggcgg gcaaaggcgt cggagaaact ctgcacgcga accagccgga
     1981 tgtcgcccag ggcgccttcg tgaatcagcc gggccatcgt ggcatcgacc gagagaccga
     2041 tcggtacagg gcacagggcg gccacgcgat cgctgtgccg cgccgcctca tacatggccc
     2101 gggcttcgct gagattccgc gccatacggg cctggcagaa cacgtgtttg cctgatttca
     2161 aggcggcgac gctgacatct ttgtgcaaat acggccacgt accgacaaac accgcgtcga
     2221 tgtcgtcccg ttcaacgagt tcctgccagg aatcgcagac gtgcgggata ccaaactgtg
     2281 cggccgcgcg ttcgctcgat tcccgggtcc gattggccac ggcaacgatc tccaccccgt
     2341 cgaggcggcg caacccaggt acgtgccggc tccggcagat gccgcccagg ccgacaaacc
     2401 caacgcgaat gctccccatt ttgaaccctc ccggctgctg gccgcttgaa aacggcttta
     2461 tccgtatcgc agacccttcg cgaacccacg cctcactgaa aaatgcggcc ccgcgcgggg
     2521 taagtccggg tctctcgcag ttatctggac agatcgagcg gccggccgtc gcgactggtc
     2581 acgcgcacac aaaattgcca gtcgtttcta ttctggaggg ctttgatgag cacgcggttc
     2641 acgccttcgg ccagcgtggc gtcgaccttg tcctggtcga ccaccattgt acggccgccc
     2701 gccgttccgt ggagccgttc cccattaatc cagagttcgc atccatcgtc gctgccaatt
     2761 tgaaaaatga catcgcgcac ctccggggaa gtcagctcgg cgcaggcata ggcggccaca
     2821 aactcggccg gatcgagccg ggcgcgcaac ccgatcacag cgggcacgct atcggtctcc
     2881 acggggagcc acgcataggt tttgccctca aaaacgaccg gttcgcggcc cgtacaggcg
     2941 gtttcgtcca ggaacgaccg ttcaaatgcc gtaccgtcag ggttggggaa cggtccgatg
     3001 atcttccaat cggttaaaaa gccctggcgc cgtgcaagca cggtgcggtc gactcccagt
     3061 gccacgagct gttcgagggc ccggcttgtc acccgggtga acagggagtt ctcggccgcg
     3121 aggaacaggc gttcccgggc tgcgtcggga ttcgtttcag cggcggcaca ggccacatag
     3181 gcgcattggg cggcttcaag cgcggccggg gtcagtgcca ggcccttcgc gcccggcgat
     3241 ggctgccaga gatccagcga atcgagatac gccgccgtcg cttcggaggg gatctgttca
     3301 aggatagcga aggcggcgac ggcttctttt tcaggaagtc cgctgtccag caagtccaca
     3361 caggcggtct gcgcggcctc cgtggcgcct tcagatgtca tggtggcaat ctgcctgaac
     3421 acggtatcga gcgcgcgggg acggatccat tccgaacccg ttctggccac tgtcaggacc
     3481 tcttcgaaag ggggcgtctc gtcagagagc aggtacgaca tcacgcgcac ttcgagggca
     3541 tcatccgtgc gggccacctc cagcaggtgc ggcagggatt ctgcatttcg cagttgcagg
     3601 atctggagca ccgccacctt tcgcggaggt tcgcattgtt cgcagacgag ggtgagcttc
     3661 tcgtcgagac ctttctgctg cgattcgacc agcgcccgga acgccacggt gctgacgccc
     3721 ggatgaagca gataacccag cgcctgagcg gcaaacgtct ccgcattcgc cgcgtccagc
     3781 ccgagaatgg attcgctaag atgggcgggt tcgttcgaat aagccgcaat gctttgatag
     3841 atcgtggccg cttcgtcatt ttcgccctgt tcacgcagcc ggtacgccgc ggccagggcc
     3901 gcctgaacat acacgacccg ctcttcgggt gtgaatgaag gccggcgaga caaaacccgg
     3961 tgcggaggca cgccgaacgc gcccagtgct ttcaggcagg caaacatcag gcggcggttt
     4021 ccgctggact gcgccagttc cgtcagcacc gggatcgcct ccggggcctg ccgccgcgcc
     4081 agggcatcgg ccatgcggag ctggacggtt tccgatgatg cagaccgcaa tgcggtgatc
     4141 agcgcccggg tcgctgtgtc gccggggatg cgttcgagcg cgatgcgtgc gtcctctcca
     4201 atagcgggat cctcaaggag gcgggccagc atgggcactt cagggtttga ggcgaccaat
     4261 ccaagatagt ggaggaattc gcagcggagc tgactgtctt cggagctttg aacggcgtca
     4321 gccagagcct gggcgaccgc gcgctgctct tcctgcgcgc cgggtctccc cgcgtacgcg
     4381 gtgataatgt cgatggcgcg caacgcggcc acgcggatct ccggctctcc cgtctccgcc
     4441 agccgggcca gcggcaaaac ggccggcgca ccgagcggtc cggccgattt ccaggctccg
     4501 gcgcgcgcgt cggcatttgg cccggcaatc cgaacgagga attcgtcgag ctcgttcgcc
     4561 tgagcagtcc agcacagcag ggcgagagcg gccatcgcca acggtgtgat ttttatggtc
     4621 agcctggcgc tacgcatggc gtgattcctt acaggtgcca cgggccgcgg cccgaatttc
     4681 ccagcatgcg gttcgcctgt tcatcgccga tgaactgctc gctggcgtgg tcccagtcga
     4741 gaggacggcc caggcgatag gccagattgg ccaggatgca cagcttggcc gcgttgtgag
     4801 cgggggcaat gtccatgatc ggccgtttac gcgacttaat gcactcaaac cagtcctcat
     4861 ggtggccggg actctggtac accggcgcac tgcccgccgg aggttcgtag gcgctcgcat
     4921 cggagctcag gccgctgccg tcgcctccgc ccacgatcag cgaacccttg tcgccgtaat
     4981 agacagcgcc gaatcgcgcg tttgagcctt tgggcacgtt gccgggctgt ttccagacga
     5041 tggtcagatt ggggtccttg aattcccagg tgacttccat ggtggtcggg cagtcataca
     5101 ggcctttgtg aggcggttcg cccacggccg tcacgcgcac cgggcctttc tcgtccagat
     5161 ccaggaacca gttcaccagg ctgagcacgt gtgcgcctcg gtcccgcacc tggcccccgc
     5221 caaaatcaag gaaccagcgg aaattggcgt gaacgcgatc ggggttatac ggcacccagc
     5281 gcgcgggcc
//
LOCUS       DAOPBQ010000230         5282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13297_length_5282_cov_3.478095, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000230 DAOPBQ010000000
VERSION     DAOPBQ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13297_length_5282_cov_3.478095"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1982)
                     /locus_tag="PKW60_08480"
     CDS             complement(54..1982)
                     /locus_tag="PKW60_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99505.1"
                     /translation="MVYEYSGYLKAILSAPDKTLRDQTEHIAFGPDDLARWTTTDLPA
                     FTEWLRIPVSRSRTDHAVELKGKFEDVRRIDYLATDEPVYWVPLGTLGVRDDRFPIDL
                     NRYPILEITCRCTSQNARLACAWAYPGGLAFDAMHSTPQWRTIARRIQHHGFPAQLDA
                     LILRLYSTVRATESAEIREVRFRAMTPAEADACERDAARLTCSAPPPHAEVLDEFVPL
                     GVFVDAQMAQQLAQTMGISLSEYWQLLFEDVVRHHQNCVAIENAERFTHDDWKEMHAA
                     ALEYGVKFVPMYGAHLMDDPAFLRDTVDELVRANADVPGLLAWGLNTEPREQDLPKLL
                     EVRDLVHDADPRHPFVLTTHNPGKLGLLAPHFAAMGVNHFHSRNSWGVQEVVETHLKL
                     SRGQQFWVVAPGFVWGSGAPEWPTCPELRLMVNSAFATGSRGFFTYLFHNVPVWFGGS
                     CQRTLTGPFLTFSDLWQELDQCMERINAMAPLLLGARPEPVPEGSYTMTLRSEEKAQL
                     PNGMPLTTASRLRGDGYDLFFLVSHDIRGMASINLSINPTFVEGLEIYDLSDFIQDRL
                     WERMPLERHLEMFPGQAHVLLIAPPDVCLHIRDIVARRLMEDDRRQLAINMRLARQYS
                     VDVSPIEKFLEETHEGDT"
     gene            complement(2018..3007)
                     /locus_tag="PKW60_08485"
     CDS             complement(2018..3007)
                     /locus_tag="PKW60_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ99506.1"
                     /translation="MRKVFGLAGLLAGLIVVACVWEYAQNGTVNFLTPRNARSLLPLI
                     GLFGILSLGQAFVVITGGIDLSVGSVVALIATLAAFLLGKGEGYSPWAVMPFLLGLSA
                     LIGLWHGLLITKARIQPFVVTLCGLFFYRGFARVIAQDTTQGFGSASTYPVWNWLANG
                     FVPTERSVLSVPFIIFLLLAGLIAVFFHFMPQGRHIFALGANEESARFSGIRTQRLKI
                     FAYTMCSFICGVGALLFGFNINSIGPATFGNFYELYAVAGVVLGGCSLRGGSGNILGV
                     VVGAALIRVLINLVTILDIPSELEYVVIGGAILVGVCVDEFLSSRRKTRISAV"
     gene            complement(3004..3969)
                     /locus_tag="PKW60_08490"
     CDS             complement(3004..3969)
                     /locus_tag="PKW60_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HPJ99507.1"
                     /translation="MRTLGTALLMAAAAIAGCGGQGGGSAEDAGKLTVAFVTNNASDF
                     WKIAQAGTEKAARELGCRVLFRIPPQGTAQEQKQIVQDLLTRGVSGIAISPKDPENQT
                     ALLNEAAAQANLVTQDSDAPDSNRICYIGTDNYQAGVAAGELLKKTLPDGGKIMLFVG
                     TLDAQNAQDRMKGIEDCLQGSNITILGTRTDETDRVKAKANAQDAITTHPELDCLVGL
                     WSYNGPAILNGVRAAGKLGEVKIVCFDEEAEALQGVQEGYIAGTIVQQPFEFGYQSVK
                     LLVELAQGDRSRVPEDQRIIVPVKTITQDTVMEFWAQLKEQTGKS"
     gene            4162..>5282
                     /locus_tag="PKW60_08495"
     CDS             4162..>5282
                     /locus_tag="PKW60_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPJ99508.1"
                     /translation="MWKSTLLLAGSLILAGAMRAGAAAEPPAPCAPVPTAEQLAWQRD
                     ELSMFVHFTVNAFTDREWGDGTEDPKIFNPVELDCEQWARVAKETGFKLVILTTKHHD
                     GFCLFPSDYTEHDIANAAWKDGRGDLVREFVEACRKYGLKVGFYLSPWDRHEPKYADN
                     AAYDEHFRNQLTELLTRYGPVSEVWFDGAGGEGHVYDWQSYYALIRRLQPRALIAICG
                     PDIRWVGNEDGFARENESSVQMRDDEEVWYPAECDVSIRPGWFWHKQQDDQVKSLEHL
                     LDIYYRSVGRNSGLLLNVPPNDKGLFCELDIRRLKEWRAALDETFATDFAAGQPAVAS
                     NVRGGDEAFGPARALDGDIDTYWATDDDVRTASLEVDLGA"
BASE COUNT         1024 a         1563 c         1714 g          981 t
ORIGIN      
        1 gatgtcgtat ccgtcgattg gcctgggtga gacactttag cgcaaaattg agcctaagta
       61 tcgccctcat gggtttcttc gaggaacttc tcgatggggc tcacatcgac gctgtattgg
      121 cgcgcgaggc gcatgttgat ggcgagctgc cgccggtcgt cttccatgag ccggcgtgcc
      181 acgatgtcac ggatgtgaag gcagacgtcc ggcggtgcga tgagtagcac atgcgcctgg
      241 ccggggaaca tctccagatg ccgttcgagc ggcatgcgtt cccagaggcg gtcctgaatg
      301 aaatccgaga ggtcatagat ttccaggcct tcgacaaaag tgggattgat gctgaggttt
      361 atggaggcca tgccgcggat atcgtggctc acgagaaaga acaggtcgta gccgtcgccg
      421 cgcagacgcg acgccgtcgt caacggcatg ccgttgggca gctgggcctt ttcttcggag
      481 cgcagggtca tggtgtacga cccttcgggc acgggttccg ggcgggcgcc caggaggagc
      541 ggggccatgg cgttgatgcg ttccatgcac tggtccagct cctgccacag gtcgctgaaa
      601 gtcagaaacg gccccgtcag cgtgcgctgg caggagccgc cgaaccagac cggcacgttg
      661 tggaacaggt aggtgaaaaa gccccggctg cccgtcgcaa aggccgaatt gaccatcaac
      721 cggagctctg gacaggtggg ccactcgggg gcgccgctgc cccagacgaa gcccggcgcg
      781 accacccaga actgctggcc ccggctgagt ttcagatggg tctcgactac ttcctgcacg
      841 ccccaggagt ttcgtgaatg gaaatggttg acgcccatcg cggcgaaatg gggtgcaagc
      901 aggccgagct ttccggggtt gtgggtagtc agcacaaagg ggtgccgggg atcggcgtca
      961 tggaccagat cgcgcacctc gagcagcttt ggaaggtcct gttcacgcgg ctctgtgtta
     1021 agcccccagg ccagaagtcc cggcacatcg gcattggctc gaaccagctc gtcgacggta
     1081 tcgcgcagaa aggccggatc gtccatcaga tgtgcgccat acatgggcac gaatttgacg
     1141 ccgtattcca gggccgcggc gtgcatttcc ttccagtcat cgtgggtgaa acgttccgcg
     1201 ttttcgatgg ccacgcaatt ctggtgatgg cgcaccacgt cttcgaagag cagttgccag
     1261 tattcgctta aggagatgcc catggtctgg gcgagctgct gcgccatttg agcgtcgaca
     1321 aagacgccca gcggcacgaa ctcgtcaagc acctccgcgt gcggcggggg cgccgagcag
     1381 gtgagccgcg cggcatcgcg ttcgcaggcg tccgcttctg caggggtcat ggcacggaag
     1441 cgcacttcgc ggatctccgc ggattccgtg gcccggaccg ttgaatacag gcgcaggatg
     1501 agcgcatcga gctgggcagg aaagccgtga tgctgaattc tccgggcgat ggtgcgccac
     1561 tgcggggtgg aatgcatggc gtcgaaggcc agtccgccgg gatacgccca ggcgcaggcc
     1621 agccgtgcgt tctggctggt gcagcggcag gtgatctcca gaatggggta gcgattgagg
     1681 tcgatgggaa accggtcatc gcggacgcca agcgttccca gcggcaccca gtagacgggt
     1741 tcgtcggtgg ccagatagtc gatccggcgg acatcctcga actttccttt caattcaacg
     1801 gcgtggtcgg tgcggctgcg ggaaacgggg atgcggagcc attcggtgaa tgcgggcagg
     1861 tcggtggtgg tccatcgggc caggtcgtcc gggccgaagg cgatgtgctc ggtctggtcg
     1921 cgcagtgtct tgtccggtgc ggacaggatg gctttgaggt agccggaata ctcgtagacc
     1981 atgcagcagc tcctttcgcg ctccgcgcgg gtccgtgcta cacggcgctg atgcgcgttt
     2041 tgcggcggct cgacaggaat tcatcgacgc agacgccgac cagaatcgcc ccgccgatga
     2101 ccacgtattc gagttcgctc gggatgtcca ggatcgtcac caggttgatc aggacccgga
     2161 tgagggccgc gccaaccacc acgccaagaa tattacccga tccgccccgc aggctgcagc
     2221 ctcccaggac gacgccggct accgcgtaca gctcatagaa attgccaaag gtggcggggc
     2281 cgatcgagtt gatgttgaaa ccgaagagaa gggctccgac gccgcagata aacgagcaca
     2341 tggtgtaagc gaagattttg aggcgttgtg tgcggatgcc gctgaaccgc gcgctttctt
     2401 cgttggcgcc cagggcgaag atgtgccggc cctggggcat gaaatggaaa aatacggcga
     2461 tgaggcctgc caacagcaga aagatgatga acggcacgga caacaccgag cgctcggtgg
     2521 gcacgaaccc gttggcaagc cagttccaga cgggataggt ggaggccgac ccgaacccct
     2581 gggtcgtatc ctgtgcaatc acccgggcaa aaccgcgata gaaaaaaagg ccgcacagcg
     2641 tgacaacaaa gggttgaatc cgcgctttgg tgatcagcag gccgtgccac aggccgatca
     2701 gagccgacag gcccagcaga aacggcatca ccgcccacgg gctgtatcct tcgcctttac
     2761 cgagcagaaa ggccgccagg gtggcgatca gggcaaccac gctgcccacc gacaggtcaa
     2821 taccccccgt gatgaccaca aacgcctgtc cgaggctgag gatcccgaac aatccgatca
     2881 ggggcaaaag gctgcgggcg tttcgcgggg tcaggaagtt aacggtcccg ttctgggcat
     2941 attcccagac gcacgcgacg acgatcaggc cggcgagcag cccggcaagc ccgaaaacct
     3001 ttctcacgat ttgcccgtct gctccttgag ctgtgcccag aattccatca cggtgtcctg
     3061 ggtaatggtc ttcaccggga cgatgatgcg ctgatcttcc ggcacgcgcg agcgatcgcc
     3121 ctgggcgagt tcaacgagca gttttaccga ttgatagccg aattcaaacg gctgttgcac
     3181 gatggtgccg gcaatgtagc cttcctgcac accctggaga gcctcggcct cttcatcgaa
     3241 gcacacaatt ttgacctcgc cgagctttcc ggcggcacgc actccgttga ggatggccgg
     3301 gccgttatag ctccacaacc ccaccaggca atcgagctcg ggatgggtcg tgatggcatc
     3361 ctgggcattg gccttggcct tcacacggtc ggtctcgtcg gtgcgggtgc caaggatggt
     3421 gatgttggag ccctgcaggc agtcttcaat gcccttcatg cggtcctggg cattctgggc
     3481 gtccagggtg ccaacgaaga gcatgatttt gccgccgtcc ggcagggtct ttttgagcag
     3541 ctcgcctgcg gccacgcccg cctggtagtt gtcggtgccg atataacaga tacggttgct
     3601 gtccggcgca tccgaatcct gtgtgactag gtttgcctgt gcagccgcct cgttgagcag
     3661 ggcggtctgg ttttcgggat ctttcgggct gatggcgatg cccgacacgc cgcgcgtgag
     3721 caggtcctgc acaatctgct tctgctcttg agcggtaccc tggggcggga ttctgaacaa
     3781 tacgcggcag cccagctctc tggcggcttt ttcggtgcct gcctgggcga ttttccagaa
     3841 gtccgatgcg ttgttggtca cgaaagcgac ggtgagcttt ccggcgtctt cagccgagcc
     3901 cccgccctgg cctccgcatc ccgcaatcgc tgcggccgcc atcagaagtg ctgttcccag
     3961 cgtacgcatt gctcatcctc ccccgggttg agatcgcatg atgttgatac gctagtgtac
     4021 acaggacgcg aaaccggtgc aaagaaaacg gagcttcgct catcggtatc cgggcaggcg
     4081 gcccgtggag ttgtgcgatc ttctgaggcc tgcgataatg ccatgcgtcc ttaccccgca
     4141 acctggacag gagcacgacc gatgtggaag agcacgctac tgctggcagg atctctgata
     4201 ctggcgggcg ccatgcgcgc aggcgcagcg gcggaaccgc cggccccctg tgcgccggtg
     4261 ccgacagccg aacagctcgc ctggcagcgc gacgaattgt ccatgttcgt ccatttcacg
     4321 gtgaatgcat ttacggaccg cgaatggggc gatggcacgg aagatccgaa gatcttcaat
     4381 ccggttgagc tcgattgcga gcagtgggcg cgggtggcga aggaaacggg atttaagctc
     4441 gtcatcctga ccacgaaaca ccacgacgga ttctgcctgt ttccgagcga ctacaccgag
     4501 cacgacattg cgaatgccgc atggaaagac gggcgcggcg atctggtgcg cgaatttgtc
     4561 gaggcctgcc ggaaatatgg tctgaaagtg ggtttttatc tgtcgccgtg ggaccgccac
     4621 gagcccaaat acgcggacaa cgccgcgtat gatgagcatt tccgaaatca gcttaccgaa
     4681 ttgctcacgc gctacgggcc cgtgtcggaa gtgtggtttg acggcgcggg cggcgaaggc
     4741 catgtctacg actggcagag ctactacgcc ctcatccgca ggctccagcc cagggccctc
     4801 atcgccatct gcggacctga catccggtgg gtgggcaacg aagacggctt tgcacgcgaa
     4861 aacgagtcca gcgtgcagat gcgtgacgac gaggaagtct ggtatcccgc cgagtgcgac
     4921 gtttccatcc gtcccggctg gttctggcac aagcagcagg acgatcaggt caagagcctc
     4981 gaacacctcc tcgacatcta ttaccggtcg gtgggacgca acagcgggct gctgctcaat
     5041 gtgccgccca acgacaaagg cctgttctgc gagctcgaca tccggcggct gaaagaatgg
     5101 cgcgcggcgc tcgacgaaac gttcgccacc gatttcgccg cgggccagcc cgccgtggcc
     5161 agcaatgtcc ggggcggcga cgaggcattt ggtcccgccc gggccctcga cggcgacatc
     5221 gacacctact gggcaaccga cgacgacgtc cggacggcgt cgctcgaggt ggatctcggt
     5281 gc
//
LOCUS       DAOPBQ010000231         5270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13334_length_5270_cov_3.537872, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000231 DAOPBQ010000000
VERSION     DAOPBQ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13334_length_5270_cov_3.537872"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3103)
                     /locus_tag="PKW60_08500"
     CDS             complement(<1..3103)
                     /locus_tag="PKW60_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99509.1"
                     /translation="MKTLWGGVMACAVLVSAAILAVPGAAAQEIDFLERFALAEDREE
                     ALRELVPGTRDYFFFHCLHFQNTGKLDEADAILQAWHRMDEHSAGLDEMRNRQALLRY
                     GGDPQLTLDYLRQKLGLHFNHERETAGAESSLPTALDPGLISRETLAQRAWAHHDGRL
                     EGFEDSALDRLADQELSGPALRECLGRLTRPDNEHLPRLVAADLRAEGNAGFGSLPIH
                     QNLLLAQLDELLALQPDLLNNGEFIAAYLARLAPGADENLQTEPAVRQAWLDRLEAFT
                     ARLSPVHNSLKACVLYQRLVHDRAQDVRDKDRFMAYLQLPRNTPYINRDFMKRPENRN
                     FTADLQQTCAGLPPIRDDQELVRDYLEHFFSTEDSYQPYAEFLDEGYLKRVFAETRIL
                     HGIGDLEQWTAWLTPGDFQQLKERVEIKFDPTNREQYGAGDPVHLDVWTKNTGTLIAR
                     VFEVNTDAFYRRYQREINTDIDLAGLVANEEQVYTYKDPPYRKVLRTLEFPGIDHRGV
                     YVIELIGNGKSSRAIIRKGALTFLSRTSTAGQVFTVLDEHGAEVKNASIWMAGHEFQA
                     DEGGYVTLPFSTQPGEQMIVLSDGTFSDLARFTHQGEEYSLSAGFILDRESLRQGGTA
                     VVGVRPVLYLNGVPVTLSVLEEPRLVISSTSNEGITSIREIPDFELFEDRLSVHEFRV
                     PAGLSTMTLTLKAKVKPFTSPEPVDLAAARAVAINQIDETQNIESVFLTRNADGYLLE
                     LFGKSGEAKGGRPLSLELKHRDFRDTVHVSLQTDGQGRARLGALADIDWLGVASPGGE
                     MHQWALANDAAAYPSILQGVSGETLYLPYTGGATAPERSELALFELRGGYMADRFANL
                     RIENRFIAMAGLEPGDYELTLKAIPHQIAVRVTKGETRLGYAMGAFRRLQEINPAPVQ
                     VTAIEPVDDAIRISIANGSSATRVHVAATRFVPAFSEFAHLGIPIERPGMSYVPAPLS
                     AYTSERAIGDEYQYILDRRYARKYPGNMLERPSLLLNPWALTETETGRQDAAEGEPPM
                     AAPA"
     gene            complement(3283..>5270)
                     /locus_tag="PKW60_08505"
     CDS             complement(3283..>5270)
                     /locus_tag="PKW60_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99510.1"
                     /translation="GSPLGIVISLGLGHWLENKPEFLRHDRDGQPYDRPNICCNFPEL
                     TPFAEHVGASVAQTFGAFPAFNAALINTEVRDGTQLCFHDHDRDLYRAKTGKEFPDAA
                     VRKNGVSYANIEGFPASRVIPDDDELLEFYRWFWREGDGWNGFHSAINNGLKSTGRSD
                     LWSFFDPAVRVPALWGSGGNVDFLSHWTYSYPDPIRIGLTADELFAMAKGGPEGQDVM
                     KMTQIIWYRSQTAPLPETGGSEGATSPWEDRDPDANYITIAPVHLREAFWTKVARPIK
                     GIMYHGWQSLVPTEGGSVYRYTHPETRYALQELTADVIQPLGPSLLQVPGARADVAFL
                     ESFTSAMFASRGTYGWGGSWGGDAYHVLLYARLQPEIIYEETIMKNGLDGFRVLVMAD
                     CDVLPEPVAERVLAFQQAGGIIVGDERLCPAIRPDIVLSVYERTKQADVDKQALLERA
                     QLLREQLGDRYRPYVDSTQAEALPYRRAFGSSDYLFAINDRREFGQYVGHHGLVMENG
                     LPISAEFTVNRAAGFVYDLLEHRQVAVESDGSVLRIPVELAPGGGGLWLITENAIDSL
                     RVDVPAEARPGQDLPVSITVADETGAAVNAVVPVEIELRDPDGRRGEFSGYYGALDGR
                     LSVTFTPAANDTPGMWTVEARELASGIAARTYFRLAE"
BASE COUNT          959 a         1589 c         1721 g         1001 t
ORIGIN      
        1 gcgcgggagc ggccatcggc ggctcgccct ccgcggcatc ctggcggcca gtctcggttt
       61 ctgtaagcgc ccacggattc agcagaagac tcggacgttc aagcatgttt cccggatatt
      121 tgcgggcata ccggcggtcg aggatgtact gatattcgtc tccgatggcg cgttccgacg
      181 tgtaagctga caggggagcc ggcacataac tcatgccggg ccgctcgatg ggaatgccca
      241 gatgcgcaaa ttcggaaaag gccgggacaa accgggtcgc ggccacgtga acccgcgtcg
      301 cggatgagcc gttcgcgatc gagattcgaa tggcgtcgtc caccggttca atggccgtga
      361 cctggacagg cgcggggtta atctcctgca gccggcgaaa tgcgcccatg gcgtagccga
      421 gacgcgtctc gcctttcgtc acacgcaccg caatctgatg cggaattgct ttgagggtca
      481 gctcgtaatc gccgggctcc aggcccgcca tggcgataaa tctgttttcg atgcgaagat
      541 tggcgaagcg gtccgccatg tagccgccgc gcaattcgaa gagcgccagt tcggaccgct
      601 caggcgcggt cgcaccgccc gtataaggca gatacagcgt ctcgccggag acgccctgaa
      661 gaatggacgg ataggctgcg gcatcgttcg ccagtgccca ttggtgcatt tccccgccgg
      721 gtgaagcgac cccgagccag tcgatatccg cgagggcgcc aagccgggcg cgcccctggc
      781 cgtccgtctg aagagacacg tggacggtat cccggaaatc acggtgtttc aattcgaggc
      841 tgagggggcg cccgcccttg gcctctccgc tctttccgaa aagctcgagg aggtatccgt
      901 cggcgtttcg ggtcagaaag acactctcga tgttctgcgt ttcgtcgatc tggttgatgg
      961 caacggcccg ggctgcggca aggtcgacag gttcggggct ggtgaatggt tttactttag
     1021 ccttcagggt cagggtcatg gtagacaggc ctgccggcac acggaattcg tgcacggaca
     1081 accggtcctc aaataactcg aaatctggta tctcccggat tgaggtaatg ccttcattgc
     1141 tggtggagga gatgaccagt ctgggttctt cgagcacaga gagcgtcacg ggcaccccgt
     1201 tcaaatagag cacgggacgc acgcctacga cggctgtgcc gccctggcgg agcgattcgc
     1261 gatcgagaat gaagcccgcc gacagcgagt actcttcacc ctgatgggtg aatcgcgcga
     1321 ggtccgagaa cgtgccgtcc gacaatacaa tcatctgctc gccgggctga gtgctgaacg
     1381 ggagcgtgac gtatccccct tcgtcggcct ggaattcgtg gcctgccatc cagatgctgg
     1441 cgtttttcac ttccgcgccg tgttcatcca gcacggtgaa cacctggccg gcggtgctgg
     1501 tgcgcgagag gaaggtgagc gcgcccttcc ggatgatcgc gcggctgctc ttgccgttgc
     1561 cgatgagttc gatgacatag actccgcggt ggtcgatacc cggaaattcc agcgtgcgca
     1621 gaactttccg gtagggcggg tctttgtacg tatacacctg ctcttcattg gcgaccagcc
     1681 cggcgagatc gatgtcggtg ttgatctcgc gctggtaccg gcggtagaag gcgtcggtgt
     1741 tcacttcgaa gacgcgggcg atcagcgtgc cggtgttctt ggtccataca tccaggtgga
     1801 ccgggtcgcc ggcgccatac tgctctctgt tagtggggtc aaatttgatc tccacacgtt
     1861 ctttgagctg ctggaaatcg ccgggcgtca gccaggcagt ccattgctcc agatctccga
     1921 tgccgtggag gatcctggtt tcggcgaaaa cacgcttaag atagccttca tcgagaaatt
     1981 cagcgtaggg ctgataagag tcttccgtgc tgaagaagtg ctcgagataa tcccgcacca
     2041 gttcctggtc gtcccggata gggggcaaac cggcgcaggt ctgctggaga tccgccgtga
     2101 agttccgatt ctcggggcgt ttcatgaaat cgcggttgat gtacggcgta ttacgcggga
     2161 gctgcaggta cgccatgaag cggtccttgt cgcgaacgtc ctgcgcgcga tcatggacca
     2221 ggcgctggta cagcacgcag gccttcagag aattgtggac cgggctcagg cgtgccgtaa
     2281 aagcctccaa ccggtcaagc caggcctgtc gaacggcagg ttcagtctgc aggttttcgt
     2341 cggcacccgg tgcgagcctt gccagatacg ccgcaatgaa ttcaccgttg ttaagaaggt
     2401 caggctggag ggcgagcagc tcatcgagct gggccagaag aaggttctga tggatgggca
     2461 gcgaaccgaa acctgcgttg ccttcggccc gaagatcggc ggccaccagg cgcggcaggt
     2521 gctcgttatc gggccgcgtg agccggccga ggcattcgcg taacgccggg ccgctgagtt
     2581 cctgatctgc gagcctgtcc agggcggaat cctcgaatcc ttccaggcgg ccgtcgtggt
     2641 gggcccatgc ccgttgagcg agcgtctcgc gggagatgag gccgggatcc agcgccgtgg
     2701 ggaggctgga ttctgcgccg gccgtctcac gttcatggtt gaaatgaaga cccagcttct
     2761 ggcgcagata gtccagcgtc agctggggat ctccgccata tcgcaggagt gcctgccggt
     2821 tgcgcatctc atcgagcccg gcggaatgct cgtccatccg gtgccaggcc tggagaatcg
     2881 cgtcggcttc gtcgagtttg cccgtgttct gaaaatgcag gcaatggaag aagaaatagt
     2941 cccgggtgcc cggcacgagc tctctgagcg cttcttcgcg atcttcggca agtgcgaagc
     3001 gttcgagaaa gtcgatctcc tgcgcggccg cccccggtac agcgagtatc gcggcgctca
     3061 ccagcactgc acatgccatc accccgcccc ataacgtttt catcgccatg cctcccgtgg
     3121 tccactctca tcaagccagg gccattctcc gccgaaacgc tgcgggattc cacccggtag
     3181 tgaaaccgtg agaaaccggt atgcagcgtt ccggcgtcca tatgacccct ttgccggccg
     3241 aaagttcgaa cggagcacct gggcgtcagg gtgtgtggcg tattattccg ccaggcggaa
     3301 atacgtgcgg gccgcaatgc cggaagccag ctcacgcgct tcaaccgtcc acatccccgg
     3361 cgtatcgttg gcagcgggcg tgaacgtgac ggataaacgg ccgtccaaag cgccgtaata
     3421 gccgctgaat tccccgcgcc ggccgtccgg gtcgcgcagc tcgatctcga caggcacgac
     3481 ggcgttcacg gccgccccgg tttcgtccgc caccgtgatc gagacgggca gatcctggcc
     3541 gggccgcgcc tcggccggaa catcaacgcg aagcgagtca atggcatttt ccgtaatcag
     3601 ccagaggcct ccgccgcccg gcgccagttc gacggggatg cgcagcacgc tgccgtcgct
     3661 ctccacggcg acctggcgat gctcaaggag gtcgtagaca aacccggccg cccggttcac
     3721 ggtgaactcg gcgctgatgg gcaagccgtt ttccatcacg aggccgtggt ggccgacgta
     3781 ctggccgaat tcgcggcggt cgttgatggc gaagaggtag tccgacgacc cgaacgcgcg
     3841 gcggtagggc agggcttccg cctgcgtgga gtccacatac ggccggtacc ggtcgcccag
     3901 ctgttcgcgc aggagctgtg cccgctccag aagcgcctgc ttgtccacgt cggcctgttt
     3961 ggtgcgttca tagacgctca ggacgatgtc ggggcggatc gccgggcaca ggcgctcgtc
     4021 tccaacgatg attccacccg cctgctggaa ggcgaggacg cgttcggcga ccggctcggg
     4081 caacacgtcg caatccgcca tgacaagaac gcggaacccg tccaggccgt tcttcatgat
     4141 ggtttcttca tagatgatct caggttgcag gcgggcatag agcagcacat ggtaggcatc
     4201 gccgccccag gagccgcccc agccgtaggt gccgcggctg gcgaacatgg ccgaggtaaa
     4261 gctctcgagg aatgcgacgt cagcgcgcgc cccgggcacc tggagcaggc tgggccccag
     4321 cggctggatc acgtcggcag tgagttcctg gagggcatag cgcgtttccg ggtgggtata
     4381 gcgatagacc gagccgccct cggtcggaac cagggactgc cagccgtggt acatgatgcc
     4441 tttaatgggg cgggccactt tggtccagaa ggcttctctc aaatggacgg gagcgatggt
     4501 gatgtagttg gcgtcggggt cgcggtcttc ccagggcgac gtggcgcctt cggaaccacc
     4561 cgtttccggg agcggagccg tctgcgagcg gtaccagatg atctgggtca tcttcatcac
     4621 atcctgccct tccggcccgc ctttggccat ggcgaagagt tcgtccgcgg tcaggccgat
     4681 ccggatcggg tcggggtagc tgtaggtcca atgtgagagg aaatcgacgt taccgccgct
     4741 gccccacagg gccgggacgc gcaccgccgg gtcgaagaag gaccacaggt cgctgcgtcc
     4801 ggtggatttg aggccgttgt tgatggcgga atggaacccg ttccagccgt cgccctcgcg
     4861 ccagaaccag cggtagaatt ccagcagctc atcgtcgtcc gggatcacgc gcgacgcagg
     4921 aaaaccctcg atgttggcat acgagacgcc attcttgcga acggcggcgt ccgggaattc
     4981 cttgccggtc ttcgcccggt acagatcccg gtcgtggtcg tggaaacaca actgcgttcc
     5041 gtcgcgcacc tccgtattga tgagggccgc gttgaatgcg ggaaaggccc cgaaagtctg
     5101 cgcgaccgag gcgcccacat gctccgcgaa cggcgtcagt tcgggaaagt tgcagcagat
     5161 attgggccga tcatagggct ggccgtcgcg gtcgtgacgc aggaactccg gcttgttctc
     5221 gagccagtgg ccgaggccca ggctgatcac gatgccgagc ggcgatccgc
//
LOCUS       DAOPBQ010000232         5258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13374_length_5258_cov_4.154718, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000232 DAOPBQ010000000
VERSION     DAOPBQ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13374_length_5258_cov_4.154718"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PKW60_08510"
     CDS             complement(<1..612)
                     /locus_tag="PKW60_08510"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPJ99511.1"
                     /translation="MRSDVVKKGFERAPHRSLLWATGQIKDREDFNKPFIAICNSFNE
                     IVPGHVHLDELGRLAKEAVREAGGVPFEFNTIGVDDGIAMGHTGMKYSLPSRELIADC
                     VETMVRAHMFDGMVCIPNCDKIVPGMLMASIRCNIPTIFVSGGPMAAGRTRDGRAIDL
                     ITVFEGVGAYKQGNLTDDELFELERNACPGCGSCSGMFTANSMN"
     gene            763..1143
                     /locus_tag="PKW60_08515"
     CDS             763..1143
                     /locus_tag="PKW60_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPJ99512.1"
                     /translation="MSKSIVIVGASTDRTKYGNKAVRAFRDGGWTVYPVNPKGGEVEG
                     LKCYTAIEDVPGPIDRVSMYVPPRHGMRMLEAIAAKKPAEFFLNPGSDDDALIERARE
                     LGLNPVQACSIVNIGLRPDMYPDE"
     gene            complement(1225..2028)
                     /locus_tag="PKW60_08520"
     CDS             complement(1225..2028)
                     /locus_tag="PKW60_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HPJ99513.1"
                     /translation="MLRQTARFCPSLLVMALLAGVPLACVEMPGNAPGKIPSCSYRVV
                     NSFPHDPEAFTQGFAFEDGCFYEGTGLRGRSSLRRVVPETGVVAQLAPLEGDYFGEGI
                     ALVGDRIIQLTWRSQIGFVYDKQTFERIGQFTYPTEGWGLTYDGQRLIMSDGSSRLYF
                     LDPETFERIGQLQVTGPRGPVNRLNELEYIDGEIYANIWRTDRIARIDPSTGEVRAWV
                     ELAGLPLPGDRSGGEDVLNGIAWDPTANRLFITGKLWPRVYEIELVETE"
     gene            complement(2028..2777)
                     /locus_tag="PKW60_08525"
     CDS             complement(2028..2777)
                     /locus_tag="PKW60_08525"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HPJ99514.1"
                     /translation="MRIRPQTRATLTGLQVLFTCSSIGSCAGMLLAYQAGNPELARAL
                     RIIACLSPVLLALVCGAHATWYGRWLFLGLLCCWLGDRLLGTYGFLPGLLAFLLGHMA
                     FAIASLARGISLKPFAVASGVLTLAAGAVLLWLYERVPPERVAPVLAYLFVISLMAAF
                     ASGASWRHRAPLLAIAASVFYVSDLFVARGMFVQYDYLNSVVGLPLYFGSLVLFALSI
                     PGATGKGSRGPLPRVVECCEEHDAIHEPEVP"
     gene            complement(2767..3504)
                     /locus_tag="PKW60_08530"
     CDS             complement(2767..3504)
                     /locus_tag="PKW60_08530"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HPJ99515.1"
                     /translation="MGYQRDAGLWGQLAGALDDWERRRIFCGVFTAFASLSIVGVVLS
                     IASQAVHPYRWGFLMFASGCFLLVAVVGGALRSVFGRLLLSGLVCCALGDYLGWHDFI
                     WGAMAFLVAHIFFVAAFWILGVDWLRALRALPLLLLVDAGVLIWLYPGIYGADRPLAF
                     AYIAVISAMVFFASGVPASRRRHLVLLAAMLFFVSDIFVARWRFTDTGSVNGYFCYPL
                     YYAACMLFAWTACWRLEEHDTAPAHAD"
     gene            complement(3879..4331)
                     /locus_tag="PKW60_08535"
     CDS             complement(3879..4331)
                     /locus_tag="PKW60_08535"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HPJ99516.1"
                     /translation="MSEAVRVIKSLSSESVAGFLLRFALGALFFFAAVGKFVGPGPAG
                     FAQYILQDFEGTYLPRVLLLPYAYVLPYVEFTLGTVLILGLFTRTSLSIAGLTLISLA
                     FGKMVQQDHATVADNLNYVFMAAVALWLAWRDNAISLDRILCRGKQAS"
     gene            complement(4412..>5258)
                     /locus_tag="PKW60_08540"
     CDS             complement(4412..>5258)
                     /locus_tag="PKW60_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPJ99517.1"
                     /translation="AIETHRVALKGPITTPIGKGFRSVNVQLRKRLNLYAAVRPVRSM
                     PGVKTRYEDVELVVIRENTEGLYSGIENEITTGVVQSLKIATRPACERIARFAFQYAR
                     ERGRQRVTVFHKANIMKLTDGLFLECARKIHEEEATDIRYDEFIVDNGCMRLVRDPGE
                     FDILLLENLYGDMISDLCAGLVGGLGVVPGANIGDDCAVFEAVHGSAPDLAGKNLANP
                     LALIMSGVMMLNHLGERDAATRIKTAYDRVLRDANPEEITRDIGGQGTTTSFTEAILR
                     RMESA"
BASE COUNT         1142 a         1609 c         1590 g          917 t
ORIGIN      
        1 gttcattgaa ttggcggtga acatgccgga gcacgagccg cagccgggac aggcattgcg
       61 ttccagctca aacagctcgt cgtcggtgag gttgccctgt ttgtaggcgc cgacgccttc
      121 aaagacggtg atcaggtcga tcgcccggcc gtcgcgagtg cggcccgcgg ccatgggtcc
      181 gcccgacaca aagatggtcg ggatattgca gcggatggat gccatcagca tgcccggcac
      241 gatcttgtcg cagttgggga tacagaccat gccgtcaaac atgtgggcgc gcaccatggt
      301 ctcgacgcag tccgcaatga gttcgcgcga cggcaggctg tatttcatgc cggtatgccc
      361 catggcgatg ccgtcgtcca cgccgatggt gttgaattca aaggggacgc cgcccgcctc
      421 gcgaacggcc tccttggcaa gccggcccag ttcgtcgagg tggacgtggc cggggacgat
      481 ttcgttaaaa ctgttgcaga tggcgataaa cggcttgttg aaatcctcgc gatccttgat
      541 ctggcccgtg gcccacagga gggagcggtg gggggcgcgc tcaaaacctt tcttgacgac
      601 atcgcttctc atggcacaac tgatccttcc gattgttgag tgacgatccg caggataagg
      661 ccttcagtat accccgctcg ctggactggt accaatttcc cggcttcggc gtggtattat
      721 cgtcaaaaag tgacacaaaa caggctgcac caggagagtc agatgagcaa gagcatcgtc
      781 attgtgggcg cgtcaaccga tcgcacaaaa tacggcaata aagccgtgcg cgcattcagg
      841 gatggcgggt ggaccgtcta tcctgtcaat ccgaaaggcg gagaagtgga aggattgaag
      901 tgctacacgg cgatcgaaga cgtgccgggc ccgatcgatc gcgtttcgat gtatgtgcct
      961 ccccggcacg gcatgcgcat gctcgaggcg atcgccgcga aaaaaccggc ggagttcttt
     1021 ctcaacccgg gttccgacga cgacgcactg attgagcgcg cccgggagct tggcctgaat
     1081 cccgtgcagg cgtgcagcat cgtgaatatt ggccttcgcc ccgatatgta tcccgacgaa
     1141 tagaggtttc cagcggtatt cttcaggcag cggataacgg cgcttgctgc ggcctctgat
     1201 gccgtcttct gaggcattca gccgctattc cgtctccacc agctcgatct catagacccg
     1261 gggccagagt tttcccgtaa tgaacaggcg gttcgcggtc gggtcccagg cgatgccatt
     1321 cagcacatct tcgccgccgc tgcggtctcc ggggagaggc aggccggcga gttccaccca
     1381 ggcccgcact tcgccggtcg acgggtcaat gcgggcgatg cgatccgtcc tccagatgtt
     1441 ggcgtagatc tccccatcga tgtattcgag ttcattcaaa cggtttacag gtccgcgcgg
     1501 gccggtaacc tgaagctggc caattcgttc aaaggtctcg ggatcaagaa aatacagcct
     1561 ggagctgccg tcgctcatga tgagccgctg gccgtcgtag gtgagccccc agccctcggt
     1621 ggggtacgtg aactgtccga tgcgctcgaa ggtctgttta tcgtacacga acccgatctg
     1681 gctgcgccac gtgagttgga tgatgcggtc gcccacgagg gcgatgcctt cgccgaagta
     1741 gtctccttcc agcggggcca gctgcgcgac gactccggtt tcggggacga cccggcgcag
     1801 tgaggatctg ccgcgcaatc cggttccttc gtaaaaacag ccatcctcaa acgcgaaccc
     1861 ctgcgtgaac gcctcaggat cgtgaggaaa cgaattgacc acgcggtagg aacaggaagg
     1921 gatctttccc ggagcgttcc cgggcatctc gacgcatgca aggggcactc cggccaggag
     1981 cgccattaca agcagggacg ggcagaatcg tgcggtttgg cggagcatca cggtacctcg
     2041 ggttcatgga ttgcatcgtg ttcttcgcaa cattctacca cgcgcggaag aggcccgcgc
     2101 gagcctttcc cggtagcgcc ggggatactc agggcgaaca ggaccagtga accgaaatac
     2161 aggggcaatc ccactaccga attcaggtag tcgtactgca cgaacatgcc ccgcgcgaca
     2221 aacaagtccg agacataaaa aaccgacgcg gcgatcgcga gcagcggcgc ccggtgccgc
     2281 caggaagccc ccgacgcgaa cgcggccatc agcgagatca cgaagagata ggcaagaacc
     2341 ggcgccacgc gctccggagg cacgcgttca tacagccaga gcaggactgc gcctgccgcc
     2401 agggtgagca ccccactggc aacggcgaac ggctttaagc tgatgcctcg tgccagggat
     2461 gcgattgcga atgccatatg gcccagcaga aaggccagca gccccggcag aaaaccgtac
     2521 gtgcccagga gccggtcgcc gagccagcaa cagagcaggc ccaggaagag ccagcgtcca
     2581 taccaggtgg catgcgctcc gcaaaccagc gccagcagta cgggcgagag gcaggcgata
     2641 atgcgcaggg cccgggccag ttcaggattg cccgcctgat aggccaggag catgccggcg
     2701 cagctcccga tcgacgagca ggtgaacagc acctgcaacc cggtcagcgt agcgcgcgtc
     2761 tgcggcctaa tccgcatggg ccggggccgt atcgtgctct tcgaggcgcc agcacgccgt
     2821 ccaggcgaag agcatacagg cggcataata gaggggataa cagaaatagc cattgaccga
     2881 gccggtatcg gtgaaccgcc accgggccac gaagatgtcg gacacgaaaa aaagcatggc
     2941 agccagcagg acgaggtggc gccgccgtga cgcgggaaca cccgacgcga agaaaaccat
     3001 cgcggagatc accgcaatat atgcgaaggc cagtggccgg tccgcgccgt aaatgcccgg
     3061 gtacagccag atcaaaacgc cggcgtcaac cagcagcagc aggggcaggg cacgcaatgc
     3121 ccgcaaccag tccacgccca ggatccaaaa tgccgcgaca aagaaaatgt gggctaccag
     3181 gaacgccatc gccccccaga tgaagtcatg ccagcccaga taatctccga gagcgcagca
     3241 gacgagtccg ctcagtagaa gccgcccgaa tacggagcgc agcgcccctc ccaccacggc
     3301 caccagcaga aaacatcctg acgcgaacat cagaaagccc cagcggtaag ggtgtaccgc
     3361 ctgcgatgcg atactcagca caacccccac gatgctcagg ctcgcgaacg ccgtgaacac
     3421 tccgcagaag atgcgcctgc gctcccaatc gtccaaagct ccggccagct ggccccagag
     3481 tccggcatca cgttgatatc ccatctgctg tgtttccgtt ctgtgtccca atgcgcccgg
     3541 cccccggcag accactgccg tcagaagaac cacgatagaa catggttcgt acgcacggca
     3601 tccgtgcgcg caccctatga cacgcttccg gcgagtctag cggcgaattc gaccaaaagt
     3661 caacaaaatg acccgccaaa ccgccagcac agatccagat ctgcagcgag gcatgccttt
     3721 gggcatgaac ggcgtgattc cggggtcttc aggccgcgct gcatctatgg agagcatacg
     3781 cgcgacaaag agcctcgcga aagcacgagg ctcccgcgca aggcagccgg ctttgctctt
     3841 aaaactcccc ctgacgcgcg tgcatcaggg acgggatgtc agctggcctg ttttccgcgg
     3901 cacaaaatcc ggtcgagtga gatggcattg tcccgccatg ccagccacaa cgccacggcc
     3961 gccatgaaca cgtaattgag gttgtccgcg acggtggcgt ggtcctgctg gaccattttc
     4021 ccgaaagcca ggctgatgag ggtgagcccg gcgatagata gtgaagtccg agtgaaaagt
     4081 cccagaatca ggacggtccc cagtgtgaat tccacgtacg gtaagacgta tgcataggga
     4141 aggaggagca cccggggcag gtaggtgcct tcaaaatcct gaagtatgta ttgggcgaat
     4201 ccggccggac cgggacccac aaatttcccc accgcggcga agaagaatag cgcacccaga
     4261 gcaaaccgca acaagaaacc ggccacactc tccgaagaaa gggatttgat gacacgcact
     4321 gcttccgaca tcgtccaacc tccattcgtt actgtcgtcc acgttctggg acgtaacacg
     4381 tacgacggaa gattcactcc ccggcgggat gtcaggccga ctccatccgg cggagaatgg
     4441 cctcagtgaa tgatgtggtg gtcccctgtc cgccgatgtc ccgggtgatt tcttccggat
     4501 ttgcatcccg taaaacacgg tcgtatgcgg ttttaatccg tgtggccgcg tcccgctcac
     4561 ccagatggtt cagcatcatc acaccgctca tgatgagcgc cagggggttg gccaggtttt
     4621 tgccggcgag gtccggcgca ctgccgtgta cagcctcaaa tacggcgcag tcgtcgccga
     4681 tgttcgcgcc gggtaccacg ccgagcccgc caacgagtcc cgcgcagagg tcgctgatca
     4741 tgtcgccgta caggttttcg agcaggagga tatcgaactc gcccggatca cgaaccaggc
     4801 gcatgcagcc attgtcgacg atgaattcgt cataacgaat gtccgtagct tcttcttcgt
     4861 ggatcttgcg ggcgcactcc agaaacaggc catccgtcag cttcatgatg ttggccttgt
     4921 gaaagacggt cacgcgttgc cgtccgcgct cgcgggcata ctggaaggcg aaccgcgcga
     4981 tgcgctcgca ggcgggccga gtggcgattt tgaggctctg caccacgcca gtcgtgattt
     5041 cgttctcgat tcccgaatac aggccttcgg tgttttcgcg gatgacgacc agttcgacat
     5101 cctcgtagcg tgttttcacg cccggcatac tgcgcacggg acgtactgcc gcatagagat
     5161 tgagccgttt gcgcagctgg acgttcacgc tccggaaccc tttgccgatc ggcgtggtga
     5221 tcggcccttt cagggccacg cggtgggtct cgatggcc
//
LOCUS       DAOPBQ010000233         5256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13379_length_5256_cov_4.152663, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000233 DAOPBQ010000000
VERSION     DAOPBQ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13379_length_5256_cov_4.152663"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..1336
                     /locus_tag="PKW60_08545"
     CDS             356..1336
                     /locus_tag="PKW60_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPJ99518.1"
                     /translation="MKYVSFGHTGIRVSPVCLGTMTFGKEADEPTSRVLMDRAFDAGI
                     NFFDTANIYNKGLTEEIVGRWLPARRDAIVLASKVHFPTGAGPNERGSSRRHILMEVD
                     KSLQRLQTGWLDILYLHHWDPDTPLEETLAALTTLVDAGKVHYCGVSNFSAWQAMKAI
                     AASRAGGWAPITCIQPMYNLVKRQAEVEILPMAVSERLAVCPYNPLAAGLLTGKYRRG
                     GTGRLDENSMYKDRYADPAYAESASRFVDYAGERGWPPAALAVAWVMTHPAVTSAIIG
                     ARNLEQLDETLGCLDISLDPVQRAEISALSIEPPPATDRTEERQVSKAAP"
     gene            1411..2367
                     /locus_tag="PKW60_08550"
     CDS             1411..2367
                     /locus_tag="PKW60_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPJ99519.1"
                     /translation="MGEFRDVVKSIVATTETVVFGKTDVVKTALAAVLAGGHVLLEDV
                     PGVAKTVLVRSLAIACGCSFRRIQCTPDLLPSDVTGVSIFNPQTREFEFRPGPIFSQM
                     LVADEINRATPRTQSALLEAMAESQVSVDGQTYKMAEPFIVFATQNPVEHEGTFPLPE
                     AQLDRFMLKLSMGYPSLIVEAKMLEETRLKHPLEALRPVADPVTIARMQRGVREVFVH
                     EKVRDYLLRIVAATRQSAHLSLGASPRASMALFRAGQALAALEGRGYVIPDDIKVLAH
                     PVMEHRLILNPESRLRRVTAYTVLHDILNQVPIPAGRAEWKP"
     gene            2481..3422
                     /locus_tag="PKW60_08555"
     CDS             2481..3422
                     /locus_tag="PKW60_08555"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPJ99520.1"
                     /translation="MMRLFARKKDAPQELDEKGEFSDEQPSLSTLELTHVGPYEIIAP
                     IGSGGMGTVYKALDRKRDQQVAIKVLDRRYDLDKKRRKQDYLGREVLIAASLEHPSII
                     RMHKEIVEQEDANGNIRRCLVMELVDGHNLRKHIKDRDLSMNQMVDLTIRLCHGLDFL
                     HQHGIVHRDIKPENFLFSRDMRKVKIVDFGLSKSNASWRTRWMKEGGGTRRYMSPEQL
                     SKEKLDARSDIFSLGITLYELFAGRHPCDGQDSREIMRQIRSSKYKFEPPSKYNPEIP
                     RQMDQIILKALRRRPERRYQSVTEMLLDLSRVSSSRI"
     gene            3496..4722
                     /locus_tag="PKW60_08560"
     CDS             3496..4722
                     /locus_tag="PKW60_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPJ99521.1"
                     /translation="MGLKKRKDDVSLTKIILGICLVVVIAFGVHRVARWFKDEYVGGA
                     LEAAAPSELARAEEAIQAGNLDEARSILLPIAEKTRNDAISPQALLLLARLEERGGDS
                     QKGLAYLKEAVEEFPGSPLNAQAELAYARALEKHGQTEDALAIFTALQENAAPEVRAP
                     ALCGLARTAERNGDLESARALCREAVRDAAWNSPDWAEAAEALGRLNIALIFSPKATS
                     DSKVYVVERGDNLTNMGMKLNTTQGLLMRANELHKDSTLNVGDRLKYTPKDFRILIER
                     SACRLFLLDGDGLFKCYSVGLGRPGHETTLGGYRIGNKQVDPTWFKPNAGPIPPGDPR
                     NELGSRWMPLVPEREELPTDLGIHGTVAPETIGKHSSSGCARMHNADVEELYDLVVRS
                     TPVEIVDTVEPRDLGL"
     gene            4887..>5256
                     /locus_tag="PKW60_08565"
     CDS             4887..>5256
                     /locus_tag="PKW60_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99522.1"
                     /translation="MEAFWDRIDELALDKSGTSKLVPVEDADLFRTLAVEAARFCEVH
                     GASGAIVLADAQTAGQVSQLAAIIQEAALAVCAGPRPDIWKKAGNVVQVKLAGLLRDH
                     RFLIVVTPSFSLALIGVQTGG"
BASE COUNT         1090 a         1625 c         1519 g         1022 t
ORIGIN      
        1 ccgcatgcat gccccgtgcg accataccgc gaagccgttc atattcccgt gaaaattgcc
       61 ggtagcctcc cgggggcttc tggtgctcgc ctccgtgcac ggttttccct ctgtctgggc
      121 aatctttcag ttgatgggca cttatttcca taaatacttt atcgtaaata agatattttt
      181 tctaccagtg caaatctgga ttgggttgtc tgtgtgtttc agatgagtca cggtcatttt
      241 gcctgatcga agcgagcgca ccgttcgact gcctggagca tgcctgccgg ctacctatcc
      301 gtgccgtgaa ttggtactat gtcggccgga ttgttgtgaa ggagacttgt ctgcaatgaa
      361 atacgtttcg tttggccata ccggcattcg ggtatccccc gtgtgcctcg gcaccatgac
      421 ttttggcaaa gaggcggatg aacccacgtc ccgcgtcttg atggatcggg cgtttgatgc
      481 cggtatcaac tttttcgata ccgccaatat ctacaacaag gggctcaccg aagaaatcgt
      541 tggccgatgg cttccggcgc ggcgcgatgc catcgttctc gcctcgaaag tccattttcc
      601 cacgggtgcg ggcccgaacg agcggggcag ctcgcgccgg catattttga tggaggtcga
      661 caaaagcctg cagcgccttc agacgggctg gctcgatatt ctctatctcc accattggga
      721 tccagacacg cctctggagg agaccctggc tgcgctgaca acgctggtcg atgctggcaa
      781 ggtccattat tgcggcgtgt ccaatttttc ggcctggcag gccatgaagg ccatcgcggc
      841 gtcccgggcg ggtggatggg ctcccattac ctgcatccag cccatgtaca acctcgtgaa
      901 acggcaggcc gaagtcgaaa tcctgcccat ggccgtctcc gaacgcctgg ccgtatgccc
      961 ctacaacccc ctcgccgcgg gcctgctgac cgggaagtac cgccgcggcg ggaccggccg
     1021 gctggatgag aattcgatgt ataaggaccg ctatgccgac cccgcctacg cagagagcgc
     1081 cagccgcttt gtggactacg ccggggagcg cgggtggccg ccggcagcac tggctgtggc
     1141 ctgggtcatg acccatccgg cagtcacgtc cgccatcatc ggcgcgcgca acctcgaaca
     1201 acttgacgaa accctcgggt gcctggatat ttcgctcgat ccagtccagc gagccgagat
     1261 ctctgcgctt tccatcgagc cgcctcccgc aaccgatcga accgaagaac gccaggtttc
     1321 gaaagccgcg ccatgagcaa ccggacgctt gcgggccatt cggtcaccgt ggaggcgccc
     1381 ggagcatccg caaaggaatg aggaaccgtc atgggggaat tcagagacgt cgttaaaagt
     1441 attgtagcca ctaccgagac cgtcgttttc ggcaagacgg acgtcgtcaa gactgccctg
     1501 gcggccgtcc ttgccggcgg gcacgtcctg ctcgaagacg tgccgggcgt ggccaagacc
     1561 gtcctcgtcc gcagcctggc cattgcctgc ggctgctcat tccggcggat ccagtgcact
     1621 cccgaccttc tgccgtcaga tgtcacgggt gtctcgatct tcaatcccca gacccgcgaa
     1681 ttcgaattcc ggccgggccc catcttctcc cagatgctcg tggctgacga aatcaaccgt
     1741 gccaccccgc gaacgcaatc ggcattgttg gaggccatgg cggagagcca ggtgtctgtg
     1801 gacggccaaa cctacaagat ggccgagcct tttatcgtat ttgccacaca gaatcccgtc
     1861 gagcatgaag gaacattccc gctcccggaa gcccagctcg accgcttcat gctcaaactc
     1921 tccatgggct accccagcct catcgttgaa gctaaaatgc ttgaagaaac ccgcctcaag
     1981 caccccctgg aggccctgcg gcctgtcgcc gacccggtca cgatcgcccg tatgcagcgc
     2041 ggtgtgcgcg aagtctttgt gcacgaaaaa gtgcgcgatt accttctgcg gatcgttgca
     2101 gccacccggc aatccgctca tctctctctg ggcgccagcc cgcgggcgtc catggccctg
     2161 ttccgggccg gccaggcgct ggccgccctc gaagggcgcg gctacgtcat tcccgacgac
     2221 ataaaagtcc tcgctcatcc tgtcatggaa caccgcctca tcctcaatcc ggaaagccgt
     2281 ttacgccgag tcaccgccta cacggtactg cacgacatct tgaatcaagt tccgatcccc
     2341 gctggccggg cggagtggaa accctaggcc gaatgctgca taatcaaagc aactttttat
     2401 gccacttctg acaccatccg ttgtatactg gaggcggcag tggcaataac tggggcaaaa
     2461 ccgatagaat cagcttgctc atgatgagac tgtttgcacg aaagaaagac gcgccgcagg
     2521 aactggacga aaaaggggag tttagtgatg aacaaccttc cctttccaca ctcgaactga
     2581 cccacgtcgg cccgtacgag attatcgcgc ccatcggcag cggcggcatg gggaccgtgt
     2641 ataaggccct ggaccgaaaa cgcgaccaac aggtcgccat caaggtcctg gacaggcgct
     2701 acgacctgga caaaaaacgg cgcaagcagg actacctcgg ccgcgaagtc ttgattgcag
     2761 cctcgctcga gcacccgtcc atcatccgca tgcacaagga aatcgtcgaa caggaagatg
     2821 ccaacggaaa tatccggcgc tgcctcgtca tggagttggt cgatggccac aaccttcgca
     2881 aacacatcaa ggatcgcgac ctgagcatga atcagatggt cgacctgacc atccgcttgt
     2941 gccacggcct ggatttcctg caccagcacg gtattgtcca ccgcgacatc aaacccgaga
     3001 acttcctctt ctcccgcgac atgcgcaaag tgaagatcgt ggacttcggc ttgtccaaat
     3061 ccaatgcgag ctggcggacc cgctggatga aggaaggcgg cgggacgcgc cggtacatgt
     3121 cgcctgagca gctttcgaaa gagaagctcg atgcccgatc cgatatcttt tctctcggca
     3181 tcacactgta cgagctgttc gcggggaggc atccctgcga cggtcaggat tcgcgcgaaa
     3241 tcatgcggca gattcggagc agcaagtaca agtttgaacc gccctccaaa tacaatcccg
     3301 aaatcccgcg gcaaatggac caaatcatcc tgaaagcctt gcgccgccgc ccggaacggc
     3361 ggtaccagtc ggtgacggaa atgttgctgg atctgtcgcg ggtctcgtcg tcgcgcatct
     3421 gatgcaggtg cgcccgccgc gtgcgggccc cggcaatgca tggcatgaga aaacataagg
     3481 aacacaggga aaagcatggg tcttaagaaa cgtaaagacg atgtcagtct gaccaaaatc
     3541 atcctgggta tctgcctggt tgtggttatt gcattcggcg tgcatcgcgt cgcgcgctgg
     3601 ttcaaggacg aatatgtcgg cggcgccctg gaagcggcgg cgccctccga acttgcgcgc
     3661 gcggaagaag cgattcaggc cggcaacctt gatgaggcgc gcagcatcct ccttccgatt
     3721 gcagaaaaaa cgcgaaacga cgccatttcc ccccaggccc tgcttcttct cgcgcggctc
     3781 gaagagcgcg gaggagacag ccagaagggc cttgcctacc tcaaggaggc cgtggaagag
     3841 ttcccgggga gcccgttaaa tgcccaggcg gaactggcct atgcccgcgc cctcgaaaag
     3901 cacggccaga ctgaagacgc gctcgccatc ttcaccgccc tgcaggaaaa cgcggcgccg
     3961 gaagtgcgcg cacctgcttt gtgcgggctg gcccggacgg cggaacggaa tggcgacctc
     4021 gaaagtgcgc gcgcgctgtg tcgtgaagcc gtgcgcgacg cggcgtggaa ctcgcccgac
     4081 tgggccgagg ccgccgaggc gcttggccgc ttgaatatcg cgctcatctt ttctcccaag
     4141 gcgacgtccg acagcaaagt ctacgtcgtc gaacggggcg acaacctcac gaacatgggg
     4201 atgaagctga ataccaccca ggggcttttg atgcgcgcca acgaactcca taaagacagc
     4261 acgctgaatg tcggcgaccg cctgaaatac acccccaaag acttccgcat tcttatcgaa
     4321 cgctccgctt gccggctctt tctgctggat ggcgacggac tcttcaaatg ctacagcgtc
     4381 ggactgggca ggccaggaca tgagaccacc ctgggggggt atcgcatcgg caacaagcag
     4441 gtggatccca cctggttcaa acccaacgcc gggcccattc caccgggcga cccccgaaat
     4501 gaactgggtt cgcgctggat gccgcttgtg cccgaacggg aggaactgcc caccgacctc
     4561 ggtattcacg gcaccgttgc tcccgaaacc atcggcaagc attcctcgag cggctgtgcc
     4621 cgcatgcaca acgccgacgt tgaggaattg tatgatcttg tcgtacgttc cacccctgtt
     4681 gagatcgttg atacggtaga gccgcgcgat ctggggcttt aagcgtccat gaaccgtttc
     4741 ccggcagtgc cggggttttg tgaacggtat ggattgactt ttgttctgca acatctgaat
     4801 aattgcgcaa acgtggcgga agtactttgg ggagaacatc ttgagactgg actgcgagga
     4861 agggggatcg accgcccggg gacatcatgg aagcgttttg ggatcgtatt gacgaactgg
     4921 ccctggacaa atccgggacc agcaagctcg tgccggtgga agatgccgac ctgtttcgca
     4981 cgctggccgt cgaagccgcc cgtttttgtg aggtccacgg cgcctccggc gcgatcgtgc
     5041 tcgccgatgc ccagaccgcc gggcaggtat ctcaattggc cgccatcatt caagaagccg
     5101 ccctggccgt gtgcgccggg ccccgcccgg atatctggaa gaaagccggc aatgtggttc
     5161 aagtaaagct cgcgggcctc ttgcgcgacc accgctttct cattgttgtg acgccatcat
     5221 tcagtctggc cctcatcggc gtccagaccg gggggg
//
LOCUS       DAOPBQ010000234         5251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13391_length_5251_cov_5.242302, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000234 DAOPBQ010000000
VERSION     DAOPBQ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13391_length_5251_cov_5.242302"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1984
                     /locus_tag="PKW60_08570"
     CDS             <1..1984
                     /locus_tag="PKW60_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99523.1"
                     /translation="PEKAQPCMRVFDQPEVPLDGRYNLPRDTDATFCMTSFASREDWE
                     AYAAALRRTILVSSALWPMPERTPLNVHIEPAAEHDDYLIEKVYFESRPGVLVTGNLY
                     RPKGEGPFPGVACPHGHWNAGRLGHEPLGSVPARCITLARMGMVAFSYDMVGYVDSLQ
                     FPNHGWEDPVHKLYGLHPFAFQLWNSMRVVDFLASLPYVDAGNLACTGASGGGTQTFA
                     LQAVEPRIKVSAPVNMISHTMQGGCMCENAPILRMRASNMEIGALMAPRPMLMVSTAG
                     DWTRLTPRVEFPAIRGIYAFYDAADRVENKHFDYPHNYNEDSRTAMYRFFGKWVLNQG
                     ARYETFTEPPFEKDPDEVLRVFPDGQLPDGFGTLDTFMPSVMEATRAKCAAMLPQNPE
                     AREAFRTGYGAALFDILGVTVPEPAQVSARRLGQGDAGGCTFERLIVWRPDCNQAIPA
                     VLYKPQGAPSGAVLAVHSQGKAALADLLAGGPGALVRAQLAAGNAVLCIDTFLTGEYQ
                     HPRTGSSRKTFKFPDTFLPTDTAFRIHDIVTAAVFLRAETGLPVTVTGLGGAGVWALF
                     AAAVEPAIAGVVADTAGFDPSSDAQWVEKHYLPCIRSVGDLNTVAAMIAPRPVELIHC
                     ADPGLWSGFQRAYANAAGTDGALTMAGGAGSTAASE"
     gene            2001..2888
                     /locus_tag="PKW60_08575"
     CDS             2001..2888
                     /locus_tag="PKW60_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ99524.1"
                     /translation="MSTTRSSIVRDRLVHIADLHFWAVVWNPFALASKRFLGNLNVML
                     RRGRHFRMERAEPFADYVASLGIPALLMTGDFTSTSTDAEFRRAREFVDGLVKRGFDV
                     SLMPGNHDVYTFREAREGRFEKYFKPYLPENGFPALRKLSGGTSLIMVPTVCANRISS
                     AGRISAEEAAATEALLREASDPVIVGAHYPALHETDAYASGINRRLRNAEALRRVVGE
                     CGKRVLYAAGHVHRFSYTRDPQFSNVTHLSSGAFFYHQPRQNCFGEFSEIDISDAGFR
                     VHRHVYRDDAWERQRVEEQ"
     gene            complement(2898..3938)
                     /locus_tag="PKW60_08580"
     CDS             complement(2898..3938)
                     /locus_tag="PKW60_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPJ99525.1"
                     /translation="MSADLMGARPRLLPVAIRFFLGILFIASAGLKIADFAAFAEQVS
                     YYGIVEAETQVRAAASLVIAIEIALGLVLLAGWWPKAVLPAIALLLLVFTGLILYAWL
                     FNDLQDCGCFGSFFAMPPAVSVGKNIVLLGLLPLAWPRTHSAAAATVLYHAPSTTAAR
                     LKAFFAGCAAGVVLALALEIGAFAVYAVFFREHILNNLESKMLAPSPPGTPMPYDWQL
                     RDEAGEPVKMDEFKDLPLFLVFFSASCPKCEAQLPALQRFYTALNDEAVAFVAVVAGN
                     VDRLDDLLARQGCTFPVYRFGETRPPLFEHFQVPSAFVFGKDGTLRYEQFDAARWDDP
                     AFVAFVNGLAEE"
     gene            complement(4106..>5251)
                     /gene="tyrS"
                     /locus_tag="PKW60_08585"
     CDS             complement(4106..>5251)
                     /gene="tyrS"
                     /locus_tag="PKW60_08585"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPJ99526.1"
                     /translation="LHIGNLVPIMGMMHFQRHGHRPIVLVGGGTGLIGDPSGKTEERK
                     LQTRENLERHLAGIRAQLGRLLAFEGDNAAIMVNNADWLGELRLLDFLRDTGKHFSVN
                     AMMAKESVRARLQDRDHGISYTEFTYSLLQAYDFLHLHDTYGCRLQVGGSDQWGNIVS
                     GMDLTRRLRQSETFGLTFPLVTRSDGAKFGKSEQGNVWLDPARTSPYKFYQFWINQAD
                     ADVPRLLRYYTFLPQEEIAALEAELAAAPEKRAAHRRLAEEVTAMIHGKEQLANAVKA
                     SEAMFGGDLSGLDDATLEDIFSEMPSTRFPREALTSGKPVLDALVESGVFPSKGEARR
                     TIKSGGVYLNNERIAEDARLSEASLCSERIAVIRKGKKNYHLLKFED"
BASE COUNT          944 a         1704 c         1650 g          953 t
ORIGIN      
        1 tccggagaaa gcccagccct gcatgcgcgt gttcgaccag ccggaagtcc cattggatgg
       61 ccgctacaac ctgcctcgcg ataccgatgc cacgttctgc atgacgtcct ttgcctcgcg
      121 cgaggactgg gaagcttacg cggcggccct tcgacgcacg atcctggtgt cgtcggcgct
      181 gtggccgatg cccgaacgga cgccgctcaa cgtccacatc gagcctgccg cggaacacga
      241 cgattacctc atcgagaagg tgtatttcga gtcgcggccg ggcgtactgg tcacgggcaa
      301 cctgtaccgg ccgaaaggcg aaggcccgtt ccccggggtg gcctgcccgc acggccactg
      361 gaacgccgga cgcctggggc acgaacccct gggctcggtt ccggcgcgct gcatcaccct
      421 ggcccgcatg ggcatggtcg cgttcagcta cgacatggtg ggctatgtgg acagtctgca
      481 gtttcccaat cacggctggg aggatcccgt ccacaagctg tatggactgc accctttcgc
      541 gtttcagttg tggaattcga tgcgcgtcgt cgattttctg gcttccctgc cctatgtgga
      601 tgccggcaac ctggcctgta ccggcgcgtc cggcggcggc acgcagacct tcgccctgca
      661 ggcggtcgaa ccgcgcatca aggtgagcgc gcccgtaaac atgatctcgc acactatgca
      721 gggcggatgc atgtgcgaga acgcgccgat cctgcggatg cgcgcctcca acatggaaat
      781 cggtgcgctc atggccccgc gccccatgct catggtgtcc accgcgggcg actggacccg
      841 gctgacgccc cgggttgagt ttcccgccat ccgcgggatc tacgccttct acgacgccgc
      901 agaccgcgtc gaaaataagc atttcgacta cccgcacaac tacaacgaag acagccgcac
      961 cgccatgtac cgcttcttcg gcaagtgggt gctcaaccag ggcgcccggt acgagacgtt
     1021 caccgagccg cccttcgaaa aagatcctga cgaggtgttg cgcgtctttc ctgacggaca
     1081 gctgcccgac ggattcggca ccctggatac attcatgcca tccgtgatgg aagccacccg
     1141 ggcgaaatgc gcggccatgc tgccccagaa tcccgaggcc cgcgaggctt tccgtaccgg
     1201 ttacggcgcg gccctgttcg acatcctcgg cgtgacggtg cccgaaccgg cccaggtgag
     1261 cgcgcggcgg ctcggccagg gggatgccgg cggctgcacc ttcgagcggc tgatcgtctg
     1321 gcggccggac tgcaaccagg ccatccccgc cgtgctgtac aaaccgcagg gcgctccaag
     1381 cggggccgtg ctggccgttc attcgcaggg taaagctgcg ctggctgacc tgctcgccgg
     1441 cggccccggc gcgcttgtgc gggcccaact cgccgcgggc aacgccgtcc tctgcattga
     1501 caccttcctc accggcgaat accagcatcc ccgaaccggc tcctcgcgca agacgttcaa
     1561 attcccggat accttcctgc cgacggatac cgcgttccgt atccatgaca tcgtgacagc
     1621 ggccgtgttc ctgcgcgcgg agacgggtct tcccgttacc gtgacggggc tgggcggcgc
     1681 gggcgtgtgg gccctgtttg ccgcggcagt cgaaccggcc attgcgggcg tcgtggcgga
     1741 cacggccgga tttgacccct cgagcgacgc gcaatgggtg gagaaacact acctgccctg
     1801 cattcgttcg gtgggcgacc tcaacacggt ggcggccatg atcgcgccgc ggcccgtcga
     1861 gcttatccat tgcgccgatc ccgggttgtg gagcggtttc cagcgggcct atgccaacgc
     1921 ggcagggacg gacggcgcgc tcacgatggc aggcggggcc ggctccaccg cggcaagcga
     1981 atagccgggg ccagcgtgcc atgagtacaa cccgctccag catcgtccgc gatcggctcg
     2041 ttcatatcgc cgacctgcac ttctgggcgg tggtctggaa cccgtttgcc ctggcgagca
     2101 agcgctttct gggcaatctc aatgtgatgc tgcgccgtgg ccggcatttc cgcatggagc
     2161 gggccgagcc ttttgcggat tacgttgcct cgctcggaat ccctgccctg ttgatgacgg
     2221 gggacttcac atcgacgtct accgacgccg aattccgccg ggcgcgtgaa tttgttgatg
     2281 ggctcgtgaa acgcgggttc gatgtctcgc tcatgcccgg caaccacgat gtgtacacgt
     2341 ttcgtgaggc acgcgagggc cgtttcgaga agtatttcaa gccctacctc cctgaaaatg
     2401 ggtttccggc gcttcggaaa ctgtccggcg gcacctcgtt gatcatggtg ccgacagtct
     2461 gcgcgaaccg gatttcatcg gcggggcgca tctccgcgga agaagcggcc gccacggaag
     2521 ccctgctgcg cgaagcttcg gatcccgtca tcgtgggcgc acattatccc gcgcttcacg
     2581 agaccgacgc gtacgccagc gggatcaacc ggcggctgcg caatgccgaa gccctgcgcc
     2641 gggttgtggg ggagtgcggc aaacgcgtcc tctacgccgc ggggcacgtg caccggttca
     2701 gctacacgcg cgacccgcag ttttccaacg tgacgcacct gtcttcaggg gccttcttct
     2761 accatcagcc ccgccagaat tgtttcgggg agttcagcga gatcgacatc tcagacgcgg
     2821 gcttccgtgt tcaccgccac gtctaccggg acgatgcatg ggaacggcag cgtgtagagg
     2881 aacagtgacc gtcccgccta ttcttccgcc agcccgttga caaacgctac aaaggccggg
     2941 tcatcccagc gggcggcatc aaattgctcg tagcgcaggg tgccgtcttt tccgaacaca
     3001 aaggctgacg gcacctggaa gtgttcgaac agcggcggac gggtttcgcc aaacctgtag
     3061 acggggaagg tgcatccctg ccgtgccagg aggtcatcca gccggtccac atttcccgca
     3121 acgaccgcca caaatgccac cgcctcgtcg ttgagcgcgg tatagaatcg ctgcagggcc
     3181 ggcagttgtg cctcacattt tggacaggat gcggagaaaa agaccagaaa cagcggaagg
     3241 tccttaaact cgtccatctt gaccggctcg cccgcctcgt cgcgcagctg ccagtcatag
     3301 ggcatgggcg tgcccggcgg tgacggcgcg agcatcttcg actcgaggtt gttcagtatg
     3361 tgctcgcgga aaaagacggc atagacggcg aaggcgccga tttcgagcgc cagggcaaga
     3421 acaacacccg ctgcgcaacc cgcgaagaac gctttcaggc gcgcggctgt ggtggaaggc
     3481 gcatggtaca acaccgtcgc ggcggccgcc gagtgtgtcc ggggccaggc cagagggagc
     3541 aggcccagca gcacgatgtt cttgccgacg gacaccgctg gcggcatcgc gaagaacgac
     3601 ccgaaacaac cacagtcctg aaggtcgttg aacagccagg cgtacaggat caggcccgtg
     3661 aacacgagca agagcagcgc gatggccggc agcacggctt tcggccacca tccggctaac
     3721 aggaccaggc cgagcgcgat ttctatcgcg atcacgagac tcgcagccgc ccggacctgt
     3781 gtttccgcct cgacgatgcc gtaataggag acttgttcgg cgaacgccgc aaaatcggcg
     3841 attttcaggc ccgccgatgc aataaacagt atgcccagaa agaatcggat cgccacaggc
     3901 agcagtcgcg ggcgcgctcc cataaggtcg gcgctcatga tggttctccc caaaccggcg
     3961 tttcaggccc ctgactgcgg gccctcgtac tcgatgcccg tcgctgcatg aaagcacaac
     4021 ccggcgaaat tcgcaatatc tggaacagcg gacagcgcag gaacgaaatc ccgcaatgcg
     4081 gccggatgct ccgcgagctg cggcgtcagt cctcgaactt caggaggtga taattctttt
     4141 tgcccttgcg aatcacggcg attcgctcgg aacacaggct ggcttccgac aggcgcgcgt
     4201 cttccgcgat gcgctcgttg ttgaggtaga ccccgccgct tttgatggtt cggcgcgcct
     4261 ctcccttgct cgggaagact ccgctctcga ccagcgcatc gagcacgggc ttgccgctgg
     4321 tcaacgcttc ccgcggaaac cgcgtcgagg gcatttcgct gaagatgtcc tccagggtgg
     4381 catcatccag cccgctcaaa tcgccgccaa acatggcctc cgacgctttg accgcgttgg
     4441 ccagctgttc cttgccgtgg atcatggcgg tcacttcttc ggccagccgg cggtgggcgg
     4501 cgcgtttttc gggcgcggcg gcaagttcgg cttccagcgc cgcgatctcc tcctgcggca
     4561 gaaacgtgta gtagcgcagc aggcgcggca catcggcatc cgcctggttg atccagaact
     4621 ggtagaactt gtagggcgac gtccgggccg ggtcgagcca gacgttgccc tgttcggatt
     4681 tgccgaactt cgcgccgtcc gaccgggtca cgagcggaaa cgtgaggcca aaggtctcgc
     4741 tctggcgcag gcgccgggtc aggtccatgc cggagacgat attgccccac tggtcgctgc
     4801 cgccgacctg caatcggcat ccgtaggtgt cgtgcagatg aaggaaatcg taggcttgca
     4861 gcaggctgta cgtgaactcg gtgtaggaaa tgccgtggtc gcggtcctgg aggcgcgcac
     4921 ggacggattc tttcgccatc atggcgttaa ccgagaaatg tttgccggta tcacgcagaa
     4981 aatcgaggag ccgtaattcg cccagccagt cggcgttgtt gaccatgatc gcggcgttgt
     5041 cgccctcaaa agccagcagc cgtcccaatt gcgcccgaat accggccagg tgccgttcaa
     5101 gattttcccg ggtctgcagt ttgcgttcct cggtttttcc gctcgggtcg ccgatcagcc
     5161 ccgtgccgcc gcccacaagc acgatggggc ggtggccgtg ccgctgaaaa tgcatcatgc
     5221 ccatgatggg gaccagattg ccgatatgca g
//
LOCUS       DAOPBQ010000235         5240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13429_length_5240_cov_4.322083, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000235 DAOPBQ010000000
VERSION     DAOPBQ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13429_length_5240_cov_4.322083"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1721)
                     /locus_tag="PKW60_08590"
     CDS             complement(141..1721)
                     /locus_tag="PKW60_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPJ99527.1"
                     /translation="MARFLLIGLDGAEPSLVERWMDAGRLPNLARLRASGVYMPCAST
                     TPPATFPAWTTCVTGVNPGKHGIFDFTAMREGTYALEFVNSTARGAPALWNLLSAAGK
                     RVGVLGVPATYPPERVNGFMVSGFDSPVCTRIDRSFVDPPNWFAEVRDWRFADFQETA
                     PGPGWHARALSRLLSGVALKEATALRLYRKEPWDFFMVVFGESDTVAHHFWLFHDPDS
                     PRHRSGFENAIASVYERLDAAVGRLVDAAGGDVTIGVVSDHGFGGAGTGVVHLNNWLA
                     AQGYLAFSGRDSSLLKRAGLTLVPESWRGALFRRLRGVAARAESASRFGGIDWAHTTA
                     WSEELNYIPSIRVNLCGREPQGQIKTADYRAFCEHLCAELRAWAPIANAWHRDELYEG
                     PYVKRAPDLILECALEDGYSHSCLRARGGLPFRRIGPDEYLGGKERGMNGNHRPTGVL
                     FLSKPSPAGYARLEDVAPTVLAELEVPAPPMDGASLLAPGQAEAAGTLSAAPERRVYS
                     QGENEAIERRLRDLGYFE"
     gene            1969..3198
                     /locus_tag="PKW60_08595"
     CDS             1969..3198
                     /locus_tag="PKW60_08595"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPJ99528.1"
                     /translation="MSPVTLTAFSGVFALGVCFSVLGSVKLKLAEQLKIDDAKVGQLI
                     SALMFSSLVFVLLIGPLTDKLGFRAIALIGFVAGAICLWLLASATTYKAALVACLLLG
                     LAAMCVNTVGNTLGPLVLFGGKNNAAASNLLNVFFGAGAFITPLIIATLLGKIGYKKT
                     VSIIGAILLIPILFAAPGTFPAPPEGFKITQALGLLARGGVWGGGLALFCYMSLEVTL
                     AGFITTYLKSHELSDEKAGTTLSGFWIALMFARLIAAFTIPAHVSAYVVPALAIVAVI
                     SIGVMVAAKSAGVGIAATLVTGFVLGPMFPTLVGVTFNKTETSGSVFGLIFGIGLLGA
                     ILMPSLIGKYAAELNIRQSLRILVGVAAALIVLSAILSFGIRDAAPALAPAEAEAAIT
                     VPATAPQAAPWQEASIR"
     gene            3411..4256
                     /locus_tag="PKW60_08600"
     CDS             3411..4256
                     /locus_tag="PKW60_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPJ99529.1"
                     /translation="MFYSGISDESGQSIDTQIRAHQELGWTHMELRLVDGQNITAVSD
                     AKFDEIYEKVSAAGMQVSCLGSAIANWARPITCDPQIDIDDLKRAIPRMHRFNCPFIR
                     VMSYPNDPDNPVSDQEWRGEAIRRMSVLAKMAEDGGITLAHENCSGWGGLSAENSNIL
                     LAEVDSPALQVVFDTGNPISYGQDSWEYYQAVYGSIVYVHIKDIRKVNGEDHYVYCGE
                     GDGCVREIVGDLLAKGYDGGLSIEPHLAAIIHTGERTTSEEKLYSTYTEYGRRLMRIV
                     EEVQP"
     gene            complement(4396..>5240)
                     /locus_tag="PKW60_08605"
     CDS             complement(4396..>5240)
                     /locus_tag="PKW60_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99530.1"
                     /translation="IGFTYDDALLTEYGRTIGMLLPHPLAPEGATHYFALLVRVAPKG
                     TSQPGELESAKGDSWIGREGLMRGFIGTVREADGSFQDSLFVVDVPADVDVTTADSGA
                     ADRYPSPPQGVKLRRLTHTWAAGNVRGMLQGDRIAYFANADDGARQVFIIPSDGSDQS
                     DEPAKRPVQATHFPGGADGGIRWHPSGNSIACIVDGGVAVTCVVPGPRFGKSVFLTPH
                     GDTERSDLVWSPDGSLLAYCRDVETLDGEGNPVRNYKGKDFGQIFTVEFPDADGDGIA
                     DTLE"
BASE COUNT         1018 a         1707 c         1594 g          921 t
ORIGIN      
        1 ctcctgggtt ccatgcccgt acaccacgag atggagcgcg tgccccgcct cgcaaagcgc
       61 ctgtgccgta tcacgaatca ggacctgcga accctgcggc accggatacg ggcatgctgc
      121 aaccatgaca atgcggcgcg tcattcgaaa tatcccaggt cgcggagccg ccgttcaatg
      181 gcctcgtttt cgccctgtga gtagaccctg cgctctggtg cggcggacaa ggttcccgcg
      241 gcttcagcct gaccgggcgc caggagactc gcgccatcca tgggcggggc cgggacctcc
      301 agctccgcga gaacggtagg ggcgacatct tcgagccgcg catagccggc cgggctgggt
      361 tttgagagaa acagcacgcc agtgggccga tggttgccgt tcatgccgcg ttccttgcca
      421 cccaggtatt cgtcggggcc aatccgccgg aacggcaggc ccccgcgtgc ccggagacac
      481 gaatgcgagt aaccatcctc cagggcgcat tcgaggatga ggtcgggggc tcgtttgacg
      541 tatggccctt catacagctc gtcgcggtgc catgcgtttg caatgggcgc ccaggcgcgc
      601 aattcagcgc acagatgctc acaaaacgcg cggtaatctg ccgtttttat ctggccctgc
      661 ggctccctgc cgcacagatt cacccggatt gatgggatat agttcaactc ttccgaccag
      721 gccgtcgtat gcgcccagtc gattccaccg aagcgggaag cgctctccgc ccgcgcggcg
      781 acgccccgca gccgccggaa caacgccccg cgccacgatt cgggcaccag agtcagcccg
      841 gcgcgtttga gcagcgagga gtccctacct gagaatgcga gataaccctg agccgccagc
      901 caattgttca gatgcaccac tcccgtgcct gcgccgccaa aaccgtggtc cgacaccacc
      961 ccaatggtca cgtcgccacc cgcggcatcc acgagccgcc ccaccgccgc atccaggcgc
     1021 tcgtagaccg acgcgatggc attttcaaag cccgatcggt gcctgggcga atccggatcg
     1081 tgaaacagcc agaaatggtg agccaccgta tccgattcgc caaagaccac catgaaaaag
     1141 tcccagggct ccttgcgata cagccgcagc gccgttgctt ccttgagcgc cactcctgaa
     1201 agcaggcggg agagtgcgcg ggcatgccag ccgggtcccg gggcggtttc ctggaaatcg
     1261 gcgaagcgcc agtcccgcac ctcggcgaac cagttcgggg gatctacaaa cgatcggtcg
     1321 atacgagtac acacgggcga atcaaagccg gacaccatga acccgttcac acgttcgggc
     1381 ggataggttg ccggaacacc cagcacgccc acgcgcttgc ccgccgctga tagaagattc
     1441 cacagcgccg gggcgccccg cgcggtactg tttacaaact cgagggcata ggtcccctca
     1501 cgcatggccg taaaatcgaa gatgccatgt tttccgggat tcacgccggt gacgcaggtc
     1561 gtccacgcgg gaaacgtcgc gggcggcgtg gtgctggcac agggcatata caccccggac
     1621 gcacggagcc gcgccaggtt cggcaggcgc cccgcgtcca tccaacgttc caccagcgac
     1681 ggctcggcgc cgtccaggcc tatcaacaga aaacgcgcca tatctttatt ctataggaaa
     1741 accatcattg gccgcagata ggcaagcagt gtaaattcgc ggccttgacg cgcaagacgg
     1801 cggaaggtac catcgtactc aacaggggac ccggtctcgg gcgactccga aagccggtgc
     1861 gtcgggagag cggcacgtgt gccccgcgcg ggggcgggtg cggagcccga tattcgccag
     1921 ccgttcccgg agggcgtcct gtatcggaca caggggaagg agaaaatcat gagtcctgtc
     1981 acattgaccg cgttttcagg tgtctttgcc ctcggggtgt gtttctccgt tctgggcagc
     2041 gtcaagctga agctggcgga acagctgaag atcgacgatg ccaaggtcgg ccagttgatc
     2101 tcggcgctga tgttcagcag cctggtgttt gtgttgctca tcggcccgct gaccgacaag
     2161 ctgggattcc gggccatcgc cctgatcggc ttcgtcgccg gcgcgatttg cctgtggctg
     2221 cttgcaagtg caacgaccta caaagccgcg cttgtcgcct gtctgctgct gggtctcgcg
     2281 gccatgtgcg tcaataccgt cggcaacacg ctcgggccac tggtcctttt cggcggaaag
     2341 aacaacgcgg ccgcatcgaa cctgctcaat gtgtttttcg gcgccggcgc gttcattacg
     2401 ccattgatca tcgcgaccct gctcggaaaa atcggctata agaaaaccgt cagcattatc
     2461 ggcgcgatcc tcttgattcc catcctgttt gcggcgcccg gcacctttcc cgctccgccc
     2521 gaagggttca agatcaccca ggcgctcggc ctgcttgctc gcggaggcgt ctgggggggc
     2581 gggctggccc tgttctgcta catgagcctc gaggttaccc tcgccgggtt catcaccacc
     2641 tatctcaaga gccatgagct ttccgatgaa aaggcgggca cgaccctgtc gggattctgg
     2701 atcgcgctga tgttcgcgcg gctcatcgcg gcgttcacca ttcccgcgca tgtgtccgcg
     2761 tatgtagtgc cggccctggc cattgtcgcc gtgatctcga tcggcgtgat ggtcgccgcc
     2821 aaatctgccg gcgtcggcat tgctgctacc ctcgtgaccg gcttcgtgct gggacccatg
     2881 ttcccgaccc tggtgggcgt caccttcaac aaaacagaga cctcgggcag cgtattcggt
     2941 ctgatcttcg gcatcggcct gctgggcgcc atcctgatgc ccagcctgat cggcaaatac
     3001 gccgcggaac tcaatatccg ccagagcctg cgcatcctcg tcggcgtagc agccgcgttg
     3061 atcgtgttga gcgcgatcct gagcttcgga attcgtgacg cagcgcccgc gttggcgccc
     3121 gcagaagccg aggcggcgat aaccgtgccc gcgacggcgc cacaggctgc accctggcag
     3181 gaggcgagca ttcgctgacg cggatccggc attcagccca catccaagca agctttccgg
     3241 gcggcgccgc tctcacgcgg acgtcgcccg gtttctttgc tccgccaccc gaagcacgct
     3301 gccggccatt tgcacgcatc cagcatgctg cggtacgatg gacttcggca atcacacaac
     3361 acagcaagcg aaagactgcc agcgcccggc gtgaccgcaa ggagttgact atgttttatt
     3421 ccggcatctc agacgaatcc ggccagagta tcgacacaca aatcagggcg caccaggaac
     3481 tgggatggac gcacatggag ctgcgcctgg tggacggcca gaatattacc gcggtttccg
     3541 atgccaaatt cgatgaaatc tacgaaaaag tctcggctgc gggcatgcag gtgtcctgcc
     3601 tgggcagcgc aatcgccaac tgggcacggc ctatcacctg tgacccgcaa atcgacatcg
     3661 atgacctgaa acgggcgatc ccgcgcatgc accggttcaa ctgccccttc atacgcgtca
     3721 tgagctaccc caacgacccc gacaaccccg tttcggacca ggaatggcgc ggcgaggcca
     3781 tccgccgcat gtccgtgctc gcgaaaatgg ctgaagacgg cggcatcacc ctcgcccacg
     3841 agaattgcag cggatggggc ggtctgtcgg ctgaaaacag caacatcctc ctcgcggaag
     3901 tcgacagccc cgcgctccag gtcgtgttcg ataccggcaa ccccatcagc tacggacagg
     3961 actcctggga gtactaccag gccgtatacg gcagcatcgt gtacgtgcac atcaaggaca
     4021 tccggaaggt gaacggcgag gaccactatg tctattgcgg cgaaggcgat ggctgcgtgc
     4081 gcgagatcgt cggcgacctc ctggcaaaag gatacgacgg cggactgtcg atcgaacctc
     4141 acctcgcggc gatcatccac accggcgaac gcaccacgag tgaggagaag ctttactcga
     4201 cctataccga atacggccgc cgcctgatgc ggatcgtcga ggaggtccag ccgtaaaacg
     4261 ccgggccgcc gaaacggcgg cgtgggatca taaacgcctc agcacctgaa aatgcgggcc
     4321 acgggtggcc cgcattgcga ttatccggca atgatgggta aaccgtcgct ttatgcccgg
     4381 ccttgcctgc tggagctatt ccagcgtatc ggcaatgccg tcgccatcgg cgtcgggaaa
     4441 ttcaaccgtg aaaatctggc cgaaatcctt gcctttgtaa ttgcggaccg ggttgccctc
     4501 gccatcgagg gtctccacgt cccggcagta agccagaagt gatccgtccg gagaccagac
     4561 gaggtcggag cgctcggtat cgccgtgggg cgtcagaaaa acgctcttgc cgaaacgggg
     4621 gccaggcacg acacaggtga ccgcaacgcc gccgtccacg atgcaggcga tcgagttgcc
     4681 actgggatgc caccggatgc cgccatcggc gccgcccggg aaatgcgtgg cctggacggg
     4741 ccgtttcgcg ggttcgtcgc tttgatctga cccgtccgac gggatgatga acacctgacg
     4801 ggcaccgtcg tccgcattgg caaaataggc gatgcggtcg ccctggagca tcccgcgtac
     4861 attccccgcg gcccaggtat gagtgagccg ccgcagcttc acgccctggg gtggtgaagg
     4921 atagcgatcc gcggcgccac tgtccgccgt ggtgacgtcc acatcggcgg gcacatccac
     4981 gacgaacagc gaatcctgaa acgagccatc cgcctcgcgc acggtcccga tgaagccccg
     5041 catgagtcct tcgcggccga tccacgaatc gcccttggca gactccagtt caccgggctg
     5101 cgacgtgcct ttgggggcga cgcggaccag cagggcaaaa taatgcgtgg cgccttccgg
     5161 cgccaggggg tgcggaagca gcatgccgat ggtgcggcca tattccgtca gcagcgcgtc
     5221 gtcatacgtg aaaccaatgc
//
LOCUS       DAOPBQ010000236         5229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13467_length_5229_cov_4.589099, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000236 DAOPBQ010000000
VERSION     DAOPBQ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13467_length_5229_cov_4.589099"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="PKW60_08610"
     CDS             <1..1576
                     /locus_tag="PKW60_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPJ99531.1"
                     /translation="PPYPALGNLVRELDSGMSGNARPAANYNDGKKLAVATAEPNLAL
                     FLNMHAYRTEMAGDSIAAVLAKDTHTGEEWRFPARWFADCTGDGSIGFLAGADYRMGR
                     ESRAETGEALAPEQPDAMTMGSSVMWYSKETDAPAPFPECPWAELFTEETAQKVTAGE
                     WDWETGLNRNQVTEIEQIRDHALRVIYGNWAFLKNHSADKEAYANRQLDWVAYMAGKR
                     ESRRLLGDVVLQQQHIEFRREFEDACVTTTWSIDLHYPEPTNTQQFPGREFRTIAEHK
                     TIVPYAIPYRCLYSRNIDNLFMAGRDISVTHVALGTIRVMRTGGMMGEVVGMAASLCR
                     KHDTTPRGVYARHLRELQALMQAGIGQAPQEVALQPPPWRDDAGPNLALAASVTVSST
                     LDEAQYPPAHINDGVIDLEDAGSRWCSARKGLPHTVDFRWDTPQTINAARIVSGWRTG
                     PHDIQGYVEAFALQVYDDGAWRDIPGTAIEGNAAHDWHARFDPVTTDRLRLEVRAAEG
                     DLARLFEVELFNLPEE"
     gene            1672..4356
                     /locus_tag="PKW60_08615"
     CDS             1672..4356
                     /locus_tag="PKW60_08615"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HPJ99532.1"
                     /translation="MFAVLLLCSMTLSGVPEAPVAAWEFNSPEEARPWRANSHMEHVS
                     VENGVLSCDTIDWDPFFTYSEAELATSPTQFVVLRIRASKAGEGQLYWTSKTEGQYGG
                     FDPSKVTNFTVEKPNVWQEIILLPFWHTEGTLRQLRLDLYEGAHFEVDWLRVYDRAEG
                     AAPASGTYRWEFDDGGDSWRLRSGSRTLVSPPLHLDISEKGWISLAVKANVATTASLF
                     WANSTGPGSHVLSFEVPASDTFQTCDIELQGESAWRDVIVALGIEAAVESGFAVQSIE
                     ITDKPLGPADIRVLHFGFENGVNRAERDSLVVARFVNDGGAASPPTSARLVLPDGLSL
                     VEGDAVQPLPALAFRERAELTWTLRSAAPGTARLLLEFPEGASVAGQTAALEFLAPMP
                     VQPADYVPEPRPVETGIDVFAYYFPGWNVGVKWNPIRFVDPIRKPLLGYYDEGLPEVV
                     DWQIKWAVENGIKGFLVDWYWSAGRQSLTHWFNAYRQARYRDQLQVAIMWANHNAPNT
                     HSAEDWRQVTREWLDQYFNLPGYYRIDGKPAVFIWAPDNIRRDLGGTDAVAQSFAESQ
                     QMARDAGYEGITYVAMGYNVSRERSDMLAREGYSGFTTYHEWGDAVKQSPVPHRAQFS
                     DVADTSVAAWDAREAIAGPLTYYPVADTGWDSRPWHGPRSLAILGRTPALFERVLRDL
                     KTWAEAHDRGMVILAPVNEWGEGSYIEPNTEFGFDMYEAVRRVFAAGEPASWPVNIGP
                     ADVARGPYEFPPRLARNRWDFSDGVQEWSALMGVSELVAQDGVLRFTTSSNDPAIQNG
                     VLDVQAAQYARMIIVMQATGKPEERMSGQLFWSETGAAITESTSIRFPLFRDGERHTY
                     TLNLSAHPRWRGEITSLRFDPCDKAGVAVAIDEIRLEK"
     gene            4467..>5229
                     /locus_tag="PKW60_08620"
     CDS             4467..>5229
                     /locus_tag="PKW60_08620"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPJ99533.1"
                     /translation="MPAETKFKRTIITGALPYANGHIHLGHIAGAYLPADIYARFKRL
                     CGHPLLYICGSDEYGVPITLTALKEGCSPQEVIDRYHAANDESFAQLGISFDIYGRTS
                     WPIHTETTQAFFLNLYQGGHIEKREMELWYSEQSDRFLPDRYVRGTCPKCGFEEATGD
                     ECENCGAQYSANDLIAPKANIPGDSSTPILKKTVHWFLRLQDFTGELTAWLDSHPEWR
                     ANVKGIAYSWVDDLRARCITRDTDWGVPIPLDDPDT"
BASE COUNT         1001 a         1602 c         1684 g          942 t
ORIGIN      
        1 gccgccgtac ccggccctgg gcaacctggt gcgcgaactg gactcgggga tgtcgggcaa
       61 tgcgcggccg gcggcgaatt acaacgacgg gaaaaagctg gctgtggcca cggcggagcc
      121 gaacctcgcc ctctttctca acatgcatgc gtaccggaca gaaatggccg gcgacagcat
      181 cgcggcggtg ctggcaaaag acacgcacac gggcgaggaa tggcgttttc cggcgcggtg
      241 gttcgccgat tgcacgggcg acggatcgat tggatttctg gcgggggcgg attaccgcat
      301 gggacgcgag agccgtgcgg aaaccgggga ggcgctggcg ccggaacagc ccgacgccat
      361 gaccatgggc tcctcggtga tgtggtattc gaaagaaacg gatgcgccgg cgccgtttcc
      421 cgaatgtccg tgggcggagc tgttcacgga ggaaacggca cagaaggtca cggcgggcga
      481 gtgggactgg gaaaccggcc tgaaccgcaa ccaggtgacc gagatcgaac agatccgcga
      541 ccacgccctg cgggtgatct acggaaactg ggcctttctc aaaaaccaca gcgcagataa
      601 ggaagcgtat gccaaccgtc aactggactg ggtggcgtat atggcgggaa aacgcgaatc
      661 acggcggctg ctgggcgatg tggttcttca acagcagcac atcgagtttc gccgcgagtt
      721 tgaagacgcc tgcgtgacaa ccacgtggag catcgacctg cattatcccg agcccacaaa
      781 cacccagcag tttccggggc gggagttccg gaccattgcc gagcacaaaa cgatcgtgcc
      841 gtacgcgata ccgtaccggt gcctgtattc gcgcaatatc gacaacctgt tcatggcggg
      901 gcgggacatt tcggtcacgc acgtcgcgct gggcacgatc cgcgtcatgc gcaccggcgg
      961 catgatgggc gaggttgtcg gcatggccgc gtcgctgtgc cggaaacacg atacgacgcc
     1021 gcgcggggtg tatgcgcgtc atctgaggga gctccaggcg ttgatgcagg cggggatagg
     1081 ccaggcgccg caggaagtgg cgcttcaacc gcctccgtgg cgcgacgatg ccgggccgaa
     1141 tctggccctg gccgcatcgg tgacggtctc gagcacgctc gatgaagcgc agtatcctcc
     1201 ggcacacatc aatgacggcg ttattgacct tgaggatgcc ggcagccgct ggtgcagcgc
     1261 ccgcaaaggc ctcccccaca ccgtcgattt ccgctgggac accccgcaga cgatcaatgc
     1321 cgcgcggatc gtgtccgggt ggcgcaccgg gccgcatgat atccagggct atgtggaagc
     1381 atttgcgttg caggtgtatg acgatggcgc ctggcgggac atcccgggga ctgcgataga
     1441 aggcaacgcc gcgcacgact ggcacgcccg gtttgatccc gtaacgaccg acaggctgcg
     1501 cctggaggtt cgcgcggccg agggagacct ggcgcgtcta ttcgaagtgg agctgttcaa
     1561 tcttccggaa gaatagaggg aatcacgggt ttcacaggtt gaaattcaat gctgcgccga
     1621 tgcggggcaa cccccaacac ctgccgcatc agccgagaga ggagaagcgc cgtgtttgca
     1681 gtgctgttgc tttgttcgat gaccttgtcg ggtgttccag aggcgccggt ggccgcgtgg
     1741 gagttcaatt cgcccgagga agcgcgtccc tggcgggcca acagtcatat ggaacatgtt
     1801 tccgtcgaga acggggtcct gtcctgcgat accattgact gggacccctt tttcacgtat
     1861 tccgaggcgg agctcgcgac cagcccgacg cagttcgtag tcctccgcat ccgcgccagt
     1921 aaggcgggcg aagggcagtt atactggact tcaaagacgg aaggccagta cggcggcttt
     1981 gatccgtcga aagtgacgaa ttttacggtg gagaagccaa acgtctggca agaaatcatt
     2041 cttctcccgt tctggcatac cgagggcacg ctccggcagc tgcggctcga tctgtatgaa
     2101 ggcgcccatt tcgaggtcga ctggctccgc gtctatgacc gcgccgaagg cgccgccccg
     2161 gccagtggca catatcgctg ggaatttgat gacggcggcg attcctggcg gctgcggtcc
     2221 ggaagccgca cgctcgtgtc tcctcctctt cacctggaca tttcggaaaa agggtggatc
     2281 tcgctcgcgg ttaaagcgaa tgtagccacg acggcgtcgc tgttctgggc caattccacc
     2341 gggcctgggt cgcatgtgct gagctttgag gtgcctgcgt ccgatacgtt tcagacctgc
     2401 gacattgaat tgcagggcga atccgcctgg cgcgatgtca tcgtggcgct cggcatcgag
     2461 gccgcagtgg aatccggttt cgccgtacaa tccatcgaaa taacagataa acccctgggt
     2521 ccggcggata ttcgcgtgct tcatttcggg tttgagaacg gtgtgaaccg cgcagagcgc
     2581 gacagcctgg tcgtcgcccg tttcgtcaat gacggcggtg cggcatctcc gcccaccagc
     2641 gcgcgcctgg ttttgccgga cgggctgtcg ctcgtcgagg gagacgctgt gcagccgctg
     2701 ccggcgcttg cgttccgtga gcgggctgag ctgacgtgga cgctccggtc ggccgctccc
     2761 gggacggccc ggctcctcct cgagttcccg gaaggtgcgt ccgtggccgg ccagaccgcc
     2821 gcgcttgagt ttctcgcgcc catgcccgtg caaccggcgg attatgtgcc cgaacccagg
     2881 ccagtggaga ccggcatcga cgtgttcgcg tattactttc cgggctggaa cgtgggcgtc
     2941 aaatggaacc cgattcgctt tgtcgatccg atccggaagc cgttgctcgg gtattacgat
     3001 gaaggcctgc ccgaggtggt cgactggcag atcaaatggg ctgtcgagaa cggcattaaa
     3061 gggtttctcg tcgactggta ctggtccgca ggacgccagt ctctcacaca ctggttcaac
     3121 gcctaccgcc aggcgcggta ccgcgaccag cttcaagtcg ccatcatgtg ggcgaaccac
     3181 aacgccccca acacccattc ggcggaagac tggcgccagg tgacccggga atggctcgac
     3241 cagtacttca acctgccggg gtattaccgt atcgacggta aacccgcggt attcatctgg
     3301 gcgcccgaca acatccgccg cgatctgggc ggcaccgacg ccgtagctca atctttcgcg
     3361 gaatcgcagc agatggcgcg cgatgccggg tacgagggca tcacctacgt cgccatggga
     3421 tacaacgtct cgcgcgagcg ttcggacatg ctcgccagag agggctacag cggttttacc
     3481 acgtaccacg aatggggcga tgccgtgaag cagtctccgg tgccccatcg cgcgcaattc
     3541 agcgatgtcg ccgacaccag cgtggccgca tgggacgccc gcgaagccat cgccggcccg
     3601 ctgacctatt atcccgtcgc cgataccggg tgggactcgc ggccctggca cggtccccgt
     3661 tcgctggcga ttctcggcag gacgcccgcg cttttcgagc gcgtgctccg cgatctcaag
     3721 acctgggccg aggcccacga ccgcggcatg gtgatcctgg ccccggtcaa cgagtggggg
     3781 gaagggagct atattgagcc caacaccgaa ttcggatttg acatgtatga ggcggtgcgg
     3841 cgggttttcg ctgccggcga gccggcgtcc tggcccgtca acatcggccc ggccgacgtc
     3901 gcacggggcc cctacgaatt tcccccgcgc cttgcgcgca accgctggga cttctccgat
     3961 ggcgtgcagg aatggtcagc gctgatgggt gtttcggagc ttgtggcgca agacggcgtt
     4021 ttgcgcttca cgacaagctc caatgaccct gccatccaga acggcgtgct ggatgtgcag
     4081 gccgcgcagt atgcgcgcat gatcatcgtc atgcaggcca cgggcaagcc ggaagagagg
     4141 atgagcggcc agctcttctg gtcggagacc ggcgccgcga ttaccgagag caccagcatc
     4201 cggttcccgc ttttcaggga tggcgagaga cacacgtaca ccctgaatct cagcgcacat
     4261 ccccggtggc gcggcgaaat cacgtccctg cgcttcgatc cgtgcgacaa ggccggcgtt
     4321 gcggtcgcca tagacgagat ccgcctggaa aaatagccgc tggcccgagc aacagcgcgc
     4381 cgcgccaaag tgtgcattac cgggcgtaaa tgcagtacaa ttacgccttt actgcgaatt
     4441 tgaagccgta acgaatggaa caagtcatgc ctgcggaaac gaagtttaaa cgaaccatca
     4501 tcacgggggc gctgccgtat gccaacggcc acattcatct ggggcatatt gccggcgcct
     4561 atcttccggc ggatatctat gcccggttca agcgcctctg cggccacccg ctgctctaca
     4621 tctgcgggtc ggacgaatac ggggtgccga tcacgctgac ggccctcaag gaaggctgtt
     4681 cgccgcagga ggtcatcgac cggtatcacg cggccaacga cgaatcgttc gcccagctcg
     4741 gcatttcatt cgacatctac gggcggacct cgtggccgat ccacaccgag acgacgcagg
     4801 cgttttttct caatctgtac cagggcggcc acatcgagaa gcgcgaaatg gagctgtggt
     4861 attccgagca gtccgaccgg ttcctgccgg accggtatgt tcgaggcacc tgccccaaat
     4921 gcggcttcga agaggcgaca ggcgacgagt gtgagaattg cggcgcgcag tattcagcga
     4981 acgacctcat tgctccaaaa gcgaatattc ccggcgacag ctcgacaccg atcctcaaga
     5041 agacggtcca ctggtttctg cggcttcagg atttcaccgg agagctcacg gcctggctcg
     5101 acagccaccc ggagtggcgc gccaacgtca agggcattgc gtacagctgg gtggatgatc
     5161 tccgggcccg ctgcatcacc cgcgacaccg actggggcgt gcccatcccg ctcgacgacc
     5221 ccgacacgg
//
LOCUS       DAOPBQ010000237         5217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13504_length_5217_cov_3.497288, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000237 DAOPBQ010000000
VERSION     DAOPBQ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13504_length_5217_cov_3.497288"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1320
                     /locus_tag="PKW60_08625"
     CDS             208..1320
                     /locus_tag="PKW60_08625"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPJ99534.1"
                     /translation="MKRREFLKKGIGAGILAGGSVFTGGCPGPAPFMPTAHAASVAAI
                     RGTNLADMARDALDAFGGAGAIVNPGERVFIKPNFGGVGMVRYNPITEGDSTKPEIVL
                     AVVEECLKAGAREVLVGEAGQVSRWNWGDVYTLDGTQTMREAVDRLNRSYRGQVILSC
                     LNSESPGWDTVPSYNRCGFVRVTELMTRADKVISLPALKTHRWTQITGAMKNFVGVTP
                     LAEYGVAGAGMRVMLHQQGIEQSFLDIVRAVQPDFTIIDCSICCEGNGPHVLPGYWGT
                     TVDMRERLGDWFLLASTDLAAADATAARVISHDPAQVNHLQMAYNQGLGQILEEEIEL
                     VGESLDTLRMYWEPAEQTQGFLEVIVPGFHLLTDSR"
     gene            1479..2660
                     /locus_tag="PKW60_08630"
     CDS             1479..2660
                     /locus_tag="PKW60_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99535.1"
                     /translation="MRFNRRQFIAASSALAVAASTRAASGKKYKACIIGDTAAGGYGH
                     YIQYAFALRDDVATVALADPDPAGRAQQAAAIGAERTYADYREMLEKERPDLVAIGPR
                     WTVKHREYLLACVEAGAHGFIEKPLCDSLASGDDMVAAVEAKNLKWAIAYNFRGMPLV
                     QHAKRLISEQGLIGTLLEARGRGKEDHRAGGEDLIVLGTHVFDLMRFFMGGSPEWCAA
                     DVTVDGRPAGPADVHEATEPLGPVVGDRIHAIYGFQGGAAGYFGSMKNPDGNGGRFGL
                     DLYGSRGIVSIRILPGDHAVSAHWFGDPSWSPGEKAAHWAPLPDAPAADMKDLQRERN
                     RIIADDLIAAIEEDRRPAFSIHDGLASWEMIQAVFESCVQGTRVTLPLTNRTHPLTRW
                     A"
     gene            complement(2752..3528)
                     /locus_tag="PKW60_08635"
     CDS             complement(2752..3528)
                     /locus_tag="PKW60_08635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPJ99536.1"
                     /translation="MCTVEPNRGCSRRSLIQCGVAAATFSALSGAAPRSHASETAPDA
                     PLQQDDVILFQGDSITDAGREKDIAEANSFRAMGQGYANMIASALLGRYASLRLQCYS
                     RGISGNKVPDLQARWQKDTIDLKPAVLSILIGVNDFWHKLGGNYDGTVADYEQGFTQL
                     LADTRAALPKVRLVICEPFVLKCGAVNDEWLPEFTERRAVAKRVAEAAGAVWVPFQTM
                     FDAAVSELMPPDYWAGDGVHPTMAGHALMAKTWLDVTGLG"
     gene            3736..>5217
                     /locus_tag="PKW60_08640"
     CDS             3736..>5217
                     /locus_tag="PKW60_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99537.1"
                     /translation="MFRLFTLTLVMATTSLWAAETKPLRAGEEAQGPWWMRETALTPT
                     GGLRLDSKPWWNDALALEVGESFLVEDGRILVRRETFTDRAGRLQDAIVCVIDDDEDG
                     SLAYGGDRDSDCYIADYNRDGTVDRMVDYIDNDGDNDPDEMDIRYFVDGELRYCWFGM
                     DLDDDDSMWSLSGYEYGGPSFFESDAYGDSMVYMNKLNTDTGTWSPISECPFAFYDTD
                     DDGYSEIVIRCSAVPLDYDPGVDPDYANDYGHFAAPWKPSLARMGIVNIRYSFDIDRG
                     SSAAMPLHFDCGFNLVGAAPYEYPGMEHFTPKRRPPQVTIVTPWEDLRRISDTYLARE
                     TGFTWHEQSDDTIAIGYGEHRELDFRWEGVFWTWERRFMENTGGPTQSWNVRREYSAR
                     PADRRELYYSGVDQRIHLKHAGEGWLRAGHFAGLGVLGEIRMFDTDGNGYFDRWEYDF
                     GDWKRVVTVADERAEDLVWDFEGLIARYTDVILPEAVAANRNFM"
BASE COUNT         1039 a         1612 c         1598 g          968 t
ORIGIN      
        1 ccctgccgaa gaacccgcat aaacccatcg ggacttcgca tcccattgaa cccgtgccgt
       61 gatgaggatt cgggccttcg tatggcgcag cattgaccgc atggccaagt aaaactagaa
      121 tctcagggga tgtgccggac tacccctgtt ttcctcaggg gaaatcctgg cccggattac
      181 gaagaggacg ctaccagcat ggatgacatg aaacgcagag aatttctgaa gaaagggatt
      241 ggcgccggca tcctggctgg cgggtccgtg ttcacgggag gatgtcccgg cccggcgccg
      301 ttcatgccga cggcacacgc ggccagcgta gcggccattc gcggcacgaa cctcgcagac
      361 atggcgcgcg acgccctgga tgcctttggc ggcgccggcg ctatcgtgaa tccgggggaa
      421 cgcgttttca tcaaacccaa ctttggcggg gtgggcatgg tacgctacaa ccccatcacc
      481 gaaggcgatt ccaccaagcc cgagattgtc ctggccgtcg tagaagagtg cctcaaggcc
      541 ggggcccggg aagtgctggt cggcgaagcc gggcaggtct ccagatggaa ctggggcgac
      601 gtctacacgc tggatggcac ccagacgatg cgcgaggccg tcgaccgcct caaccgaagc
      661 taccgcggcc aggtcatctt gtcctgtctg aacagcgaaa gcccggggtg ggacaccgtc
      721 ccgtcgtaca acaggtgtgg cttcgttcga gtgaccgagc tgatgacgcg cgctgacaaa
      781 gtgatctcgc ttcccgctct gaaaacccat cgctggaccc aaattaccgg cgccatgaaa
      841 aactttgttg gagtcacccc gctcgcggaa tacggcgttg cgggcgccgg tatgcgggtc
      901 atgctgcacc agcagggcat tgaacagtct tttctggaca ttgtccgcgc tgtgcagccg
      961 gattttacca ttatcgactg ttccatctgc tgcgaaggca acggacctca tgtgctgccc
     1021 ggatactggg gaacaaccgt cgacatgcgt gagcgtcttg gcgactggtt cctcctggca
     1081 agcacagatc tggctgcggc ggatgcgaca gccgcccgcg ttatcagcca cgatcccgcg
     1141 caggtgaatc acctgcagat ggcctacaac cagggcctgg ggcagatcct cgaggaggaa
     1201 attgagctgg tcggcgaaag cctggacacc cttcgcatgt attgggagcc cgccgagcag
     1261 acccagggtt tcctggaggt cattgttccg ggattccacc tccttaccga cagccgttga
     1321 cgcagaatac gggaatgtcc ggccgcatct gcaccatctt ctgttcgatt tgcgcgcagc
     1381 agccctgcag ccgccttctg aaccaaatgt gcgcttgacg ccgttttgcg atgcacttat
     1441 gctgccctca gcgcacctca aaacacggag acctggcaat gcgtttcaac cggcggcaat
     1501 tcatcgcggc gtcgtctgca ctggccgtgg ctgcgtcaac acgcgcggcg tcggggaaga
     1561 aatacaaagc ctgcatcatc ggcgataccg cggccggcgg ctacggccat tacatccagt
     1621 acgcgtttgc gctgcgggac gatgtcgcca ctgtcgcgct ggcggacccg gacccggccg
     1681 ggcgggcgca gcaggccgcg gcgatcggcg cggaacgcac gtacgccgac taccgcgaga
     1741 tgctcgaaaa ggagcggccg gatctggtcg cgatagggcc gcggtggacg gtaaaacatc
     1801 gcgagtacct tctcgcgtgc gtcgaggccg gagcccacgg tttcattgag aaacctcttt
     1861 gcgacagcct ggccagcgga gacgacatgg tcgcggcggt tgaagccaaa aacctgaaat
     1921 gggccatcgc ctacaatttt cgaggcatgc cgctggtgca gcacgcgaag agattgatca
     1981 gcgaacaggg gcttatcggc acgcttctgg aagcacgcgg acgcggcaag gaagaccacc
     2041 gcgcgggcgg cgaagacctc atcgtgcttg gcacgcacgt gtttgatcta atgcgcttct
     2101 tcatgggcgg ttctccagag tggtgcgccg ccgatgtcac ggttgacggc cgcccggccg
     2161 gtccggccga cgttcacgag gccaccgaac cgctcggacc cgtcgtgggt gaccgcattc
     2221 atgccatcta cgggttccag ggcggcgccg ccggatattt cggttccatg aaaaaccccg
     2281 acggaaacgg cggccgcttc ggtctcgatc tgtatggaag ccgcggcatc gtctccattc
     2341 gtattctccc cggcgaccat gccgtgagcg cccactggtt tggcgatccc tcctggtcgc
     2401 ccggggaaaa ggccgcccac tgggcgccgc ttcccgacgc ccccgccgct gacatgaagg
     2461 atctccagcg cgagcggaac aggatcatcg cggacgacct cattgccgcc atagaagagg
     2521 accgccggcc ggcattcagc attcatgacg gcctcgcctc atgggagatg attcaggcgg
     2581 tgtttgaatc gtgcgtccag ggcactcgcg tgacattgcc cctgaccaac cgtacgcacc
     2641 ccctcacgcg ctgggcctga tccgcccgtc gcgcctgatg tgcgagattc agcgcccgga
     2701 gcctggagcg ccgctcagag aatcaacagg aggagtacgg cctcgcgagg ttcaccccag
     2761 cccggtcaca tcaagccagg tcttcgccat cagcgcatgg ccggccatgg tgggatggac
     2821 tccgtcgccc gcccagtagt ctgggggcat gagctcgctc acggccgcat caaacatcgt
     2881 ctggaacggc acccatacgg caccggccgc ctccgcgacc cgtttggcca cggcccggcg
     2941 ttccgtgaat tccgggagcc attcgtcatt caccgcaccg catttcaata cgaaaggctc
     3001 gcaaatgacc aggcggactt tcgggagggc cgcgcgcgtg tccgccagga gttgtgtgaa
     3061 gccctgctca taatcggcca cggtgccgtc gtaatttccc ccgagtttat gccagaaatc
     3121 gttgacccca ataagaatgc tcaataccgc gggtttgaga tcgatggtgt ccttctgcca
     3181 gcgtgcctgc aggtcaggca ccttgtttcc actgatgccg cggctgtaac actggagccg
     3241 caggctcgca tacctgccca ggagagccga cgcgatcatg ttcgcatagc cctggcccat
     3301 cgcgcgaaag ctgttggcct ctgcgatgtc cttctcccgc ccggcatccg tgatcgagtc
     3361 gccctgaaac aaaatgacgt cgtcttgctg caggggggca tccggcgcag tttccgacgc
     3421 gtgcgagcgc ggcgcggcgc ccgagagagc ggaaaaggtc gcggcggcaa ctccgcattg
     3481 aatcagactg cgacgcgaac atcctcgatt cggctcaacg gtacacattg cacgtactcc
     3541 ttcaatcctc gcggccagtt aaggcgcgtt aacatccccc aaacatgcgc tgtaatctcc
     3601 acataataca caatgagagg tgacgccgca caaacagaac gattggcctg cgaagatccg
     3661 cggttattgg ccggtgcacc ggaggcggcc ggctgcggat agacattcac gaggcaactg
     3721 actatgattc cctccatgtt cagattattc acattgaccc tcgttatggc cacaacttcg
     3781 ctgtgggccg cggaaaccaa gccgttacgc gccggtgaag aagcgcaagg accgtggtgg
     3841 atgagagaga cggccttgac ccccacgggc gggctgcggc tggacagcaa gccctggtgg
     3901 aacgatgcgc tcgctctgga agtgggcgaa tcctttcttg tcgaggacgg acgaattctt
     3961 gtgcgccgcg agacgtttac ggaccgcgcc ggccgcctcc aggacgccat cgtgtgcgtg
     4021 atcgacgacg acgaagacgg cagtctcgcc tatggcggcg accgggattc cgattgctac
     4081 atcgctgatt acaaccgcga tggcacggtt gaccgcatgg tcgactacat cgacaacgac
     4141 ggcgacaacg acccggacga gatggacatc cgctattttg tcgacggcga acttcgctat
     4201 tgctggttcg gcatggacct ggacgacgac gattccatgt ggagcctgag cggctatgaa
     4261 tatggcggcc ccagcttttt cgaaagcgac gcgtacggcg acagcatggt ctacatgaac
     4321 aaattgaaca ccgatacggg cacctggtcg cccatatcgg agtgtccgtt tgccttctac
     4381 gacaccgatg acgatggcta cagcgagatc gtcatccggt gcagcgcggt accgctcgac
     4441 tacgatcccg gcgtcgaccc cgactatgcc aacgactacg gccattttgc tgccccctgg
     4501 aaaccgtctc ttgcccgcat gggcatcgtc aatatccgct acagttttga tattgaccga
     4561 ggcagttcgg ccgccatgcc gctgcacttt gattgcggct tcaacctggt cggagcggcg
     4621 ccatacgagt atccgggcat ggagcacttc actccgaagc gccggccgcc gcaggtcacg
     4681 atcgtcacgc catgggaaga tctgcgccgc atctccgata cgtatctcgc gcgcgaaacc
     4741 gggttcacct ggcacgaaca atccgacgac accatcgcga ttggctatgg agaacaccgg
     4801 gaactggact tccgctggga gggggtcttc tggacctggg aacggcggtt catggaaaac
     4861 acgggcgggc cgacacagag ctggaatgtc cgccgtgaat acagcgcccg gccggcggac
     4921 agacgcgaac tgtattacag cggcgtagat cagcgcatcc atctgaaaca tgctggagaa
     4981 ggctggctgc gggccggcca tttcgcgggc cttggcgttt tgggtgagat ccggatgttt
     5041 gacaccgacg gcaacggcta ttttgaccgg tgggagtacg atttcggcga ctggaagcgg
     5101 gtggtgaccg tggccgacga acgtgccgaa gatctggttt gggactttga agggctcatt
     5161 gcacgatata ccgatgtcat cctgcctgaa gccgtggcgg ccaaccgaaa tttcatg
//
LOCUS       DAOPBQ010000238         5207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13531_length_5207_cov_4.050660, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000238 DAOPBQ010000000
VERSION     DAOPBQ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13531_length_5207_cov_4.050660"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKW60_08645"
     CDS             <1..180
                     /locus_tag="PKW60_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99538.1"
                     /translation="APRYVLQQFRPDKEIALRDPRLAEPPHTAQWFDARVAELNPQFP
                     DVLTRGVRQRAVSTG"
     gene            complement(199..480)
                     /locus_tag="PKW60_08650"
     CDS             complement(199..480)
                     /locus_tag="PKW60_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HPJ99539.1"
                     /translation="MVHAFVLASVKRDQINETAQELLKIDGVAEVYSIAGDWDLIIII
                     RARENDQLAAIVTEHMLKLDGLVNTTTLIGFRAYSNYDLDRMFSIGFQD"
     gene            866..1606
                     /locus_tag="PKW60_08655"
     CDS             866..1606
                     /locus_tag="PKW60_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPJ99540.1"
                     /translation="MKDMSRSRKSPRPRGTHGVYRKPNPRVQTTTLWDFPSQQYGTGG
                     QGDQEYRGATPAYVIWNLLQRYTKPGDMVVDPMCGSGTTLDVATELGRRALGFDVAPY
                     REDIQPADARKLPIRNDQADFVFMDPPYSTHIDYSDDPRCIGKLDAAGPEYYAAMAVV
                     IKEAARILKPDRYLALYCSDSYRKGKPFCPIGFELFGLLCERLEPVDIVAVTRRNRTL
                     MRRHYHTEAEKGNYFLRGFNYLFIMHKP"
     gene            1884..2726
                     /locus_tag="PKW60_08660"
     CDS             1884..2726
                     /locus_tag="PKW60_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPJ99541.1"
                     /translation="MTRLAETKNMAIFCDFENIALGVRDAEYAQFDIEKVLERLLLKG
                     NIVVKKAYCDWARYKDHKTAMHEASFELIEIPHVRQSGKNSADIRMVVDALDLCYTKE
                     HVDTFVIISGDSDFSPLVSKLRENNKTVIGVGVRNSTSDLLIANCDEFISYDDLVSAP
                     RSRIKAGSSPASRKQPRAAKGKAAPPLDEKKQEAWNLIVSTYEALLEERGEEERIWGS
                     MVKQALKRRNPGFSETHYGFRSFGQLLEEAEAHGILRLERDEKSGGFIFKATHDPVRR
                     KGDS"
     gene            3271..4083
                     /locus_tag="PKW60_08665"
     CDS             3271..4083
                     /locus_tag="PKW60_08665"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPJ99542.1"
                     /translation="MTRFAGLAKTMALVTLLFGVHAAHATMIDLTTPGASGILNGAIF
                     QQTSLQPTGTGVIHSFAQIGKANKSVVHGYNTTVNKVFDNRSSDNFNHSITVGMIPVV
                     SLDGIDYYQFLLDINESKGGLNRYLSLDEVQIFVGGPANPSSTAFDSNGILQGVGTLV
                     YDMENNAGNWVALNYALNSGSGSGDMFLYVPTRNFGGAADDAVVTLYSRFGGQGMNPV
                     EYGAGNFGNSAGFEEWALVQGEKQSIVPEPATLSLIGLGLAGFAVRQVARRR"
     gene            complement(4159..>5207)
                     /locus_tag="PKW60_08670"
     CDS             complement(4159..>5207)
                     /locus_tag="PKW60_08670"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99543.1"
                     /translation="WTCRDFRELNARDDIDAVIIGTPDHWHALTSIDAARHGKDIYCE
                     KPLTWSLGEGQAVVRAVRENSRILQVGSMQRSDARFKRACEIVRNGYLGKVTHINVSL
                     PDYGNHVWVDEFPKAPDFLDWEFYVGPATWTPFHPKRYDWDWRWWMAFGGGQMMDWIG
                     HHADIAHMAMDWDNTGPRVITGVRWDMLNERNNLYDAPARYRFECEYKGGVTMTVANA
                     SDMPEEFKQAAGDRLGTQFIARDGAWIYVDRGEFRASSGELRKLTMRGNDFRFRPEGN
                     HMRDFLDCVKSRQEPSAPVNAGHRSASVGHLGKIACQLGATLHWDPKTETFPKEPSLN
                     ALLTRQYRGEWTLA"
BASE COUNT         1052 a         1459 c         1645 g         1051 t
ORIGIN      
        1 gccccacgct atgtgctcca gcagtttcgc ccggacaagg agattgcgct gcgtgacccc
       61 cggcttgcgg aaccgcctca tacggcccaa tggtttgacg cgcgtgtcgc ggagctcaat
      121 ccacagttcc ccgacgtgct cacccggggc gtcaggcaac gcgcggtgag caccggctga
      181 cgcgctgcgc ggcgccggct aatcctggaa tccgatgctg aacatgcgat cgaggtcgta
      241 attgctgtag gcgcggaacc cgatgagcgt ggtggtgttc accagaccgt cgagcttcag
      301 catgtgttcg gtcacgatgg ccgcgagctg gtcgttttcg cgcgcgcgaa tgatgataat
      361 caggtcccag tcaccggcga tggaatacac ttcggcgacg ccatcgattt tgagcagttc
      421 ctgggcggtc tcgttgatct ggtcccgctt gacactcgcc agcacgaacg catgtaccat
      481 gaaaatcgcc tcctgttgcg tgaacggcgc ggtctcacgg catgccgcac cggcagggct
      541 taccactgcc cgctatggta agcgcccggc aagggtgagt caacgcggct gcggtgactg
      601 atgtatggca gcaagtccct gccgcagaat ccgggaggag aggccgcgaa gagccgctcg
      661 agttgacacg ttggtccggg cggtggcagg ctcgaaaggc tgcagacttg gagaggatcg
      721 cgattttgtc cggcaggatc cgcggacggg cgcccagccg tcacggctgc gggacgtgtt
      781 gcgaatgagt ctgccggggg aacacaatca ggccgaaggt taaggatggg aatacgccgg
      841 ccaccaggtc cggggatggg ttcagatgaa agacatgtcg cgcagccgca aatctcctcg
      901 cccacgagga acgcacggcg tataccgaaa acccaacccc agggtgcaga ccaccacgct
      961 gtgggacttt ccgtcgcagc aatatggcac gggcgggcag ggagaccagg agtatcgtgg
     1021 cgcgacaccg gcctacgtga tctggaatct gctgcaacgg tatacgaaac cgggcgatat
     1081 ggtggtggat cccatgtgcg gcagcggcac aaccctcgac gtggccacgg aactcggccg
     1141 gcgcgcgctg gggtttgacg tggcgccgta ccgcgaagac atccagccgg ccgatgcccg
     1201 aaagctgccc attcggaacg atcaggccga ttttgtcttt atggatcccc cgtattccac
     1261 gcacattgat tacagcgacg atccgcgctg catcgggaag ctggacgcag ccggacccga
     1321 gtattacgcc gccatggctg tcgtgatcaa ggaagcggcg cgcatcctca aacccgaccg
     1381 ctacctggcg ctgtattgtt cggattcgta ccggaaaggc aagccattct gcccgattgg
     1441 gtttgagctg ttcggtctct tgtgcgagcg cctggagccg gtggacatcg ttgccgtcac
     1501 gcggagaaac cggaccttga tgcggcggca ctaccatacc gaagccgaaa agggaaacta
     1561 ctttctccgg ggcttcaact acctgttcat catgcacaag ccataagcgg cggtgtgcat
     1621 tgccgggatg ccggcgcttt ggcgccgctg cgaaaccggg ccaacgcacc tccgtgcgat
     1681 gcgtcggcga atgcaggcgc tggacctttc gaagatctga gtcagggcag tttaaacacg
     1741 cgtgcgggtc ctgcccgctc aggctgcaat gcgccctctc gcacgggttt tggttgacga
     1801 atcgccgggc gagcgccaca atgcagagca gcaggtccgg gccccgcgta tgagggaacc
     1861 cgcctgcaaa aggagttgta attatgacgc ggcttgccga gaccaagaac atggccatct
     1921 tctgcgactt tgagaacatc gccctgggcg tccgggacgc ggagtacgcc cagttcgata
     1981 ttgaaaaagt gctggagcgg ctgctgctca aagggaacat cgtagtgaag aaggcgtatt
     2041 gcgactgggc gcgctataaa gaccacaaaa cggcgatgca cgaagcctcc tttgaattga
     2101 tcgaaatccc gcatgtgcgc cagtccggca agaactccgc ggacattcgc atggtggtcg
     2161 acgcgctgga tctttgctat acgaaagagc atgtggacac ctttgtgatc atcagcgggg
     2221 attcggactt ttccccgctc gtgagtaagc tgcgggaaaa caacaagacc gtgatcggcg
     2281 tgggcgttcg gaattcgact tccgacctgc ttattgccaa ttgcgatgag ttcatcagct
     2341 atgacgacct ggtttcggcg ccccgctcgc gcatcaaggc aggcagcagc ccggccagcc
     2401 ggaaacagcc gcgggcggcg aaaggaaaag ccgctccgcc cctcgatgag aaaaaacagg
     2461 aagcatggaa cctgattgtc agcacctacg aggccttgct ggaggaacgt ggcgaggaag
     2521 agcggatctg gggttccatg gtgaaacaag ccctcaaacg ccgtaacccc gggttcagcg
     2581 agactcatta tgggttccgc tccttcggcc agctgctcga agaagccgag gcgcatggca
     2641 tcctgcgact ggaacgcgac gaaaaatcgg gcggctttat cttcaaagcc acccatgatc
     2701 cagtccggcg aaaaggcgat tcctgaacat tcgaaacgtg agggcggccg gcctgcccca
     2761 tcctgcaaat cccccggcga ccccgtctga tctttgcatg cgtgccgcac gggctcatca
     2821 aataattcga acaggacgga gccgatacac ggccacgtta ccgcagtctt cacatgtgcc
     2881 tgcaatgcgt agcggcggtg caatcctgaa cggaaagatg aagacggcga gacggcccgt
     2941 taattatcaa cagttagtca caattgaaca atatattgac tatcaggggc cgtttgctta
     3001 gattttgact tcttcacgag actcttcagg ccgtttggat gggatatccg gggctgtaaa
     3061 tcaagaattc ttgcatccaa gtgttcggga taaatccttt gttcattgga ccatatgcgg
     3121 cttttccagt ggcaggtcag agaattttgc tgcgcttccg cccgaaatca aggaattggc
     3181 aagatacttg ctaatccttc actgaagttt acggtgtgag ttggctgcgg tcaaggcaag
     3241 gccaggtttt tcaccagggg gttttgaatc atgacgcggt ttgctggttt ggcgaagacg
     3301 atggcgctcg tgacgctgct gtttggcgtg cacgcggcgc atgccacgat gattgatctt
     3361 acgacgccgg gcgcgagtgg gatattgaac ggcgccattt tccagcagac cagtctgcag
     3421 ccgaccggca cgggtgtgat tcactcgttc gcacagattg gcaaagccaa taaatccgtg
     3481 gtgcacggtt acaataccac cgtgaacaag gtgttcgaca accgttcttc cgacaatttc
     3541 aaccattcga tcaccgtcgg aatgatcccc gtggtctcgc tggatggcat tgactactat
     3601 caatttctgc tggacatcaa cgagagcaag ggcgggttga accggtacct ttcgcttgat
     3661 gaggtccaga tttttgtcgg cggtccggcg aatcccagct cgacggcctt tgacagtaac
     3721 ggcattctgc agggcgtggg cacgctggtg tatgacatgg agaacaacgc cggcaactgg
     3781 gtcgctctga actatgctct gaattcaggg agcggcagcg gcgacatgtt cctctatgtg
     3841 ccgaccagaa attttggcgg cgcggccgat gatgccgttg tgacgctgta ttcacgtttc
     3901 ggcgggcagg gcatgaatcc ggtggaatac ggtgcgggta atttcggcaa ctcggccggt
     3961 tttgaggagt gggcgctggt gcagggtgaa aagcagagca tcgttccgga gccggctaca
     4021 ctgtcgctga ttgggctcgg tctggcgggg tttgcggtcc gccaggtcgc gcgcaggcgc
     4081 tgagcagcag atttcgccgg agcagtatga tccggggacg tgacgcacgc atcacgtccc
     4141 cggttttttg tgtgcgggtc aggcgagggt ccattctcca cgatattggc gtgtcagcag
     4201 ggcgttgagc gagggctctt tggggaaggt ctcggtcttg ggatcccagt ggagggtggc
     4261 gccgagctgg caggcgattt tgccaagatg cccgactgag gcggaacggt ggcccgcatt
     4321 gacgggcgcg ctcggttcct ggcgcgattt gacgcaatcg agaaagtcgc gcatgtggtt
     4381 gccttccggg cgaaaccgga aatcgttgcc ccgcatggtg agtttccgaa gctcgccgct
     4441 gctggcacga aactcgcccc gatcgacata gatccaggca ccgtctctcg cgatgaactg
     4501 cgtgccgagg cgatcgcccg cggcctgttt gaattcctcc ggcatatcgc tggcgttggc
     4561 gacagtcatg gtgacgccgc ccttgtactc gcattcgaag cggtaacggg cgggggcatc
     4621 gtagaggttg ttacgctcgt tgagcatatc ccagcgcacg cccgtgatga cccgcggacc
     4681 cgtgttatcc caatccatgg ccatgtgagc gatgtcggcg tggtggccga tccagtccat
     4741 catctggccg ccgccgaagg ccatccacca gcgccaatcc cagtcgtagc gcttggggtg
     4801 gaagggcgtc caggtggccg ggccaacgta gaattcccaa tcgagaaaat ccggggcctt
     4861 cgggaattca tcaacccaga cgtgattgcc gtaatcgggc aggctgacgt tgatgtgcgt
     4921 gactttgccc agatacccgt ttcggacgat ttcacaggcg cgtttgaaac gcgcatccga
     4981 gcgttgcatg ctgccgacct gcagtatgcg gctgttttcg cggacggccc tgacgacagc
     5041 ctggccttcg cccagcgacc aggtcagggg cttttcacag tagatgtctt tgccgtgccg
     5101 ggcggcgtcg atggaggtga gggcgtgcca gtggtcgggt gtgccgatga tcacggcgtc
     5161 gatatcgtcg cgggcgttga gttcgcggaa atcgcggcag gtccagc
//
LOCUS       DAOPBQ010000239         5189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13587_length_5189_cov_3.609077, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000239 DAOPBQ010000000
VERSION     DAOPBQ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13587_length_5189_cov_3.609077"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3535)
                     /locus_tag="PKW60_08675"
     CDS             complement(<1..3535)
                     /locus_tag="PKW60_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99544.1"
                     /translation="MAVFLMVALQALDAPAQDVLHRHAAHTMLRDIAIDGSSGAVFVA
                     AYAREEIWKLSATGEPIATAKTGSGPVALALSLDGRLVACVNKLSASVMVFDAESLTL
                     RKTFACGEGAAAVAAAGNSAFAIANSLADTVTVVELESGKTALLEDVGRVPNAVAFSD
                     SVLAVAARQPAAITLLDLSTGLEAGPRRVTKLPAAPLAVAAETGGRFVVATETALLRV
                     DGHTGEVLASTALAASDVAVSGERIVALSGDFLVMLDPALDSLERIPIGAHATRVDSS
                     GGLVGLVAPAEAAWLLKGAPEDWQKELPVQQELTPLEPATSESVQVLPAEPAPGEPAA
                     ATPEPVSPVPVRSPGPLPEESPGPALPEVVEAEPARESKAGKSPSAPSEAASAVAAQH
                     EGQPAALQDATEQDEKAPHESKASPEPEEQSSGTPEAQAADETAEDLRQPGISPYAQR
                     PRTIATFGTGAPQFTREAPEGTPLAGAWSGSLSEAFTRGWDFANFKGAFELQDWNQPL
                     EFRGVKADRFKGTPGEDGKLQRMSFQGNVSWDWAESAFRAQLLEADQVTREILVQDAH
                     VERELSSLDADRLYYRYPSQYDEAIMPLIESQSLTTQEQARRLYTLGYGEADNIELVE
                     PFRELRAKHAQYNFAEKTGAATEVRGRFDVLHFGIDELEMLGPGHIQAHDAWITTCPG
                     DPPIFRLRLKEMEILEDNAVVGRKARLQIGKVITPIYWPKWSFHPGRDKLIDIDFESG
                     RAAELGYYLNYGQRFALSRNADLGFRFIPTEKEGVGLGLEGQYDYMLKPSSPLFRGKG
                     EFRSLATTEERGYFEAYHRHELTEDTVLLLQAEYWNDENIIKDFYYNEYRNRTEPRNF
                     ANVTHTRPTYLATGTFRYNENEVFAETERLPEATYHLLERQLAKRLYLSFDTIAGFNE
                     REPSGTHAWRMANVARASMDLDVADTFSVVPFYEADLSWYSRTVDDGDSDLRFANTVG
                     VTAQSRFHRAYPGALGFSGFKHVIVPSLTYSYRPEPTMDVDETPRFDAYDNVYGRSRI
                     EGKVENILFARDALTGAVWQAARLTLYHGNDFWNEIRGSTDYELELDLRPRPWWGILT
                     VTEHHSIGNDFDLDEPYLFERLALQFWEDMMGKPYDPELAFQYNAVYGDYDRVLSYLY
                     YDDLAQGGRFGARLGFAYTETQSRVFNREVLY"
     gene            complement(3957..4892)
                     /locus_tag="PKW60_08680"
     CDS             complement(3957..4892)
                     /locus_tag="PKW60_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPJ99545.1"
                     /translation="MQFELITLGCACADVIARPVDQFPEFGHLQLLPELEIHLGGLAA
                     VTATVFSQLGGKSALIGKLGADGFGDYIIGALNQAGVNTTHVRREKSEHTSATIVLVT
                     SQGERTFLHHLGTNGTTTPDDIDMGLARQAKAFHWGGPGVTPALTGKGMGKVFRELRE
                     AGVMTSMDTCFDGSGTWFPLIEEALPHLDVVMSSLHEARYYTGQQEPRDIVRFFRSFG
                     AKYVMLKLGGDGMIVEDEEASHHLEAHKVNVVDTTGAGDASCAGFLYGLLNGWGLRRS
                     GQLANAVGGLTVQHMGGAEAITSLEETIAFMERGA"
BASE COUNT          973 a         1574 c         1574 g         1068 t
ORIGIN      
        1 catagaggac ttcgcggttg aacactctgc tctgagtctc ggtgtacgcg aaacccaggc
       61 gggcgccaaa acgtccgccc tgggccaggt cgtcgtaata gagataggag aggacccgat
      121 cataatcgcc gtacaccgcg ttgtattgaa aggccagttc ggggtcgtag ggtttgccca
      181 tcatgtcctc ccagaactgg agggcaagcc gttcaaacag gtacggttca tcgaggtcga
      241 aatcgttgcc gatgctgtga tgctcggtga cggtcaagat gccccaccac gggcgcggcc
      301 tgagatccag ttccagttca tagtcggtgg aacctcggat ctcgttccaa aagtcgttgc
      361 cgtggtacaa ggtcagacgc gccgcctgcc agaccgcgcc ggtgagcgcg tcacgtgcga
      421 acaggatatt ctcgaccttc ccctcaatac ggctccggcc gtacacgttg tcgtaggcat
      481 cgaaccgggg tgtctcgtcg acgtccatgg taggttccgg gcggtaggaa tacgtcaggg
      541 acggcacgat tacgtgcttg aatcccgaga aaccgagcgc tcccggatac gcgcggtgga
      601 agcgtgattg cgccgtcaca ccgacggtgt tggcgaatcg caggtcggaa tccccgtcat
      661 ccaccgtgcg cgaataccac gacaggtcgg cctcgtaaaa cggcacgacg ctgaatgtat
      721 ccgcgacgtc gagatccata ctggcacggg caacattagc catgcgccag gcatgagtgc
      781 ccgacggttc gcgttcgttg aatccggcaa ttgtgtcaaa gctgaggtac agccgtttgg
      841 cgagctgccg ttcaagcaag tggtacgtcg cttccggcag gcgttcggtc tctgcaaaga
      901 cttcattctc gttgtagcgg aaggtgcccg tggccagata cgtgggccgg gtatgggtta
      961 cattggcgaa attccggggt tccgtccggt tccggtattc gttgtaatag aaatccttga
     1021 tgatgttctc atcgttccag tattcagcct gcagcagcag caccgtatct tcggtgagtt
     1081 cgtgccggtg gtacgcctcg aaatagcccc gttcttccgt ggtcgcgagg gaacggaatt
     1141 cgcccttccc acgaaacaga ggcgaactgg gcttgagcat gtaatcgtac tggccttcca
     1201 gcccgagccc caccccttct ttttccgttg ggataaagcg gaatcccagg tcggcattcc
     1261 ggctcaatgc aaagcgctgc ccgtagttca ggtagtagcc gagttcagcg gcccggccgc
     1321 tttcgaaatc gatatcgatc agcttgtccc gtccggggtg gaagctccat ttcggccagt
     1381 agatgggtgt tatgaccttg ccgatctgga gccgcgcttt tcggccgacg acagcgttgt
     1441 cttccagaat ttccatttct ttcaggcgga ggcggaagat gggcgggtcg ccggggcagg
     1501 tggttatcca ggcgtcatgc gcttgaatgt gtcccggccc cagcatttcc agctcgtcaa
     1561 ttccgaaatg gagcacatcg aagcgcccac gcacttcggt ggcggctccg gttttttccg
     1621 cgaaattgta ctgggcatgt tttgcgcgca gttcccggaa aggctcgacg agttcaatat
     1681 tgtcggcctc gccgtacccc agcgtgtaca ggcggcgggc ctgttcctgc gtggtaaggc
     1741 tctgggactc gatcaaaggc ataatggctt cgtcgtactg cgatgggtag cgataataga
     1801 gccggtcggc gtcaagcgag gagagttccc gctccacatg ggcgtcctgg acgagaatct
     1861 ctcgcgtgac ctggtcggcc tcgagtaatt gggcccgaaa tgccgattcc gcccagtccc
     1921 acgacacatt gccctgaaac gacatccgct gaagtttgcc gtcttccccg ggcgtgcctt
     1981 tgaagcggtc ggccttgacg ccgcggaatt cgaggggttg gttccaatcc tgcagttcaa
     2041 acgcgccctt gaaattggcg aaatcccagc cgcgcgtaaa cgcctcgctg aggcttccgg
     2101 accatgcgcc cgccagcgga gtgccttcgg gggcctcgcg tgtgaactgc ggcgcgcctg
     2161 tcccgaatgt ggcaatggtg cgggggcgtt gagcgtaggg gctgattccg ggctggcgaa
     2221 ggtcttcagc ggtttcgtcc gctgcctggg cttcaggtgt gcccgaagac tgttcctccg
     2281 gctccggcga cgctttcgac tcgtgcggag ctttctcgtc ctgttcggtt gcgtcctgga
     2341 gtgcggcggg ctggccttcg tgctgggccg ccacggccga tgctgcttca gagggcgccg
     2401 acggggattt cccggctttg ctttcccggg ccggctccgc ctccaccacc tctggaagcg
     2461 ccggccccgg gctctcctca ggcagtggtc cgggtgagcg cacgggcacc ggcgagacgg
     2521 gctcaggcgt ggcggccgcc ggttcgcccg gcgccggttc tgccggcaga acctggaccg
     2581 attcggaggt ggcgggttcc aggggagtga gctcctgttg aacgggcagc tctttctgcc
     2641 aatcttccgg cgcacccttc aacagccagg cggcttcggc tggggcgacc agacctacaa
     2701 gcccgcctga cgaatccacg cgtgtcgcgt gcgcgcctat ggggatgcgc tccaggctgt
     2761 cgagcgcagg atccagcatc accaggaagt cgccgctcaa ggcaacgata cgttcccccg
     2821 atacggcgac atctgaggcg gcgagggcgg ttgaagcgag cacttcgcct gtatggccgt
     2881 ccacccgcag cagtgcggtt tcggtggcca cgacaaatct gccgccggtt tccgctgcga
     2941 cggcgagggg cgccgcaggg agctttgtaa cgcgccgcgg cccggcctct aaccccgttg
     3001 acaggtccag cagggttatt gcggcgggct ggcgagcggc aacggccagc acggagtcgg
     3061 aaaaagccac cgcgttggga acgcggccca catcttcaag aagagccgtc ttgccacttt
     3121 ccagttccac aacggtaacc gtatcggcaa ggctgttggc aatcgcaaat gcgctgtttc
     3181 cagccgcggc gactgccgcg gcgccttcac cgcacgcaaa tgtcttgcgg agcgtcaggg
     3241 actctgcatc gaagaccatc acgctggcag acagtttgtt gacgcacgcg acgaggcggc
     3301 catcaaggct caatgccaga gcaaccggtc cgctcccggt tttggccgtc gctatgggtt
     3361 cccctgtggc gctcagcttc cagatttctt cccgcgcata agcagcgacg aatacggccc
     3421 cgctggaacc gtctatcgcg atatcgcgga gcatggtgtg ggccgcgtgg cggtgaagga
     3481 catcttgcgc aggggcgtcc aaagcctgca gcgcaaccat cagaaaaaca gccattctcc
     3541 cgagtctggc gcatgatgct gtgaacactg gctcaagtct ccggaaggat aaaccagaaa
     3601 gcggcgacgg cccggtgagc gcgggcgtct tcgggaatca gcggacgcgg cgctgcggat
     3661 tgctcttccg agggggcgct tttttctgag ggcgttgcct cccgggtctc ccatgtttcc
     3721 cgaggcggga caacgggggc gctctcctga ggcggaggcg cggcggtcct cggtggttct
     3781 tcccggcggg gctgtccctc gcggcctgac atagaactgc tcggagtcaa atccgtactg
     3841 gtaacaatcc cgtcatcgag ttgccccagc acaatcgccg ccacggtcgc aatcagaaat
     3901 gcttttgtca tcgcttccgc tcccagccct catattgaac ggaaatgtgt ctcgaatcaa
     3961 gcgccacgtt ccataaaggc gatggtctct tccagggagg ttatggcttc agcccctccc
     4021 atatgctgca cggtgaggcc gcccacggca ttggcaagtt gaccgctgcg gcgcaggccc
     4081 catccattga gcaaaccgta gaggaatccg gcacaggagg catccccggc gcctgtggtg
     4141 tctaccacgt tgactttgtg cgcttccagg tgatgcgatg cttcctcatc ttcgacgatc
     4201 attccgtcgc cacccagctt caacatcacg tatttggcgc caaacgagcg gaaaaaccgg
     4261 acaatgtcgc gcggctcctg ttggccggtg taataccggg cttcatgcag gctcgacatg
     4321 accacgtcga ggtggggcag cgcctcttca atcaacggga accaagtgcc tgacccgtca
     4381 aaacaggtgt ccattgacgt catgacgccg gcctcgcgca actcccgaaa cactttcccc
     4441 atccctttgc cggtgagggc gggcgtgacg cccgggccgc cccagtgaaa ggcttttgcc
     4501 tgtctggcca gtcccatatc gatgtcgtcc ggcgtggtgg ttccgttcgt tcccaggtgg
     4561 tgcaggaacg ttcgctcccc ctgggaggtc accaggacaa tcgtggcgga ggtgtgttct
     4621 gatttttccc gccgcacgtg ggttgtattg acaccggcct gatttaaggc cccaatgatg
     4681 taatcgccaa acccatccgc gcccagtttt ccaatcaggg ccgatttccc gccgagctgg
     4741 gaaaacacgg tggccgtgac agccgccaga ccgcccaggt gaatttccag ttccggcaac
     4801 aattgcagat gaccgaattc cggaaactga tccacgggac gggcaatgac gtccgcacat
     4861 gcacaaccga gagtgatcaa ttcgaattgc atgataaccc cacttctccc ccatgcttgg
     4921 ctgattgtct cgataccacg acgatacgaa aaatgaacag caaagctccc atcgcgttca
     4981 cagcgttcca gttagcaccc tgacgaatga aattgagtat agcacggcct tttcaacggc
     5041 acaaaatacc aacgtcaatt gttgccgaaa ctccgttttt cgatgccttt ttgatcttcg
     5101 agagccctgc ggtccagcgt gcaacgggaa gctggtgttc agcaggcctc tttggcgcat
     5161 cgacacaccc aaatgaaaca acaactgac
//
LOCUS       DAOPBQ010000240         5132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13760_length_5132_cov_4.853851, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000240 DAOPBQ010000000
VERSION     DAOPBQ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13760_length_5132_cov_4.853851"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..969)
                     /locus_tag="PKW60_08685"
     CDS             complement(190..969)
                     /locus_tag="PKW60_08685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPJ99546.1"
                     /translation="MPASRWIVFPLLLCAVFLCTRAVCADSSALPAADSASAPPPGVK
                     TVYLTFDDGPSELTPALLDVLKQHEAAATFFVCGNVTEFGNRMYNRILDEGHALGNHT
                     FSHQYRTVYKSADAFEKNVARLDDLIFRFTGTRPRLLRFPAGSNNRITRGPDGKSITH
                     ELVDRMAKLGYRHVDWNVDSLDHGVNASSSEAIAKTVIEGVLKRDQAIVLMHDSYTHA
                     ATVEAVPVIIRELRSKGYIFSPLSETSYTVQFLKSQPANGP"
     gene            complement(1068..2237)
                     /gene="metK"
                     /locus_tag="PKW60_08690"
     CDS             complement(1068..2237)
                     /gene="metK"
                     /locus_tag="PKW60_08690"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPJ99547.1"
                     /translation="MSQQTKGVLFTSESVTEGHPDKLADFISDSILDAVIEQDPMSRV
                     ACETMVTTGFCVIAGEITTKAVADYPKVVREAIAAIGYAGGDSGFDGRSCGVLVSLDK
                     QSPDIAQGVDAATNKEQGAGDQGMMFGYACDETPEYMPLPIALAHKLGLKLSELRHNG
                     SLPWLQPDGKSQVTVEYRNGKPHRIHTIVISNQHSPDISQQDIRDAVIRQVCEPVVPK
                     ELVNGDIIYHVNPTGRFVVGGPAGDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVD
                     RSGAYMARYMAKNVVAAGLASRCEVQLAYAIGVARPVSIHVTTFGTGALSDDALSTLL
                     AEEFDCRPAAILELLDLRTPIFRKTTNYGHFGRENAGLRWENTGHVEALRQRAGI"
     gene            complement(2392..3102)
                     /gene="rplA"
                     /locus_tag="PKW60_08695"
     CDS             complement(2392..3102)
                     /gene="rplA"
                     /locus_tag="PKW60_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPJ99548.1"
                     /translation="MSSQSKRRKALNEQNLKQIACTLDEAATGVKACATAKFDETVEL
                     AFTLGVDPRHADQMVRGTVSLPHGTGKQVRVVVFCKETEQINAAREAGAIDAGAEELL
                     QKVQGGWTDFDVAVSTPDMMRDVGKLGKVLGPRGLMPSPKAGTVTAKVGEAVTEILKG
                     KIDFRVDKNANIHVPIGKASFAVEQIRENAAAVVEAVVKARPAACKGTYLKSCCMSST
                     MGPGFRLDAPSLVSQFRA"
     gene            complement(3190..3630)
                     /gene="rplK"
                     /locus_tag="PKW60_08700"
     CDS             complement(3190..3630)
                     /gene="rplK"
                     /locus_tag="PKW60_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPJ99549.1"
                     /translation="MAKGKKGKNVAALIKLQCPAGQANPAPPVGPALGQHGVNIMDFC
                     KQFNERTKDQQGLVIPAVITVYEDRSFSFITKSPPAAVLIKRAAKLAKASGEPNRNKV
                     GSVRKAELVEIAEIKKDDLNAADTDAAVRMLAGTARSMGILVEN"
     gene            complement(3661..4614)
                     /gene="nusG"
                     /locus_tag="PKW60_08705"
     CDS             complement(3661..4614)
                     /gene="nusG"
                     /locus_tag="PKW60_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPJ99550.1"
                     /translation="MTDEQNDKKPQKLDEELRERQSERAAKDAAEREFAASFFGDVKK
                     ESSQEEDAAEAEARKALTEDLRREQEAQEAQEAEAELQARRFLAGDGGGTPAKSVEVE
                     VRDLGTGADSNDEGDDEAPKEDPILATLKPDPDDGVRRRWYALHAYSGQEGNVRKNLL
                     MQAELDGLDDLIGGVLVPMEQVAEIKGGEKRISKRKFFPGYVLVRLPEHPERRPELWH
                     LINNTPGVSGFIGSRHTPVPLEDAEVNAIIDEIRGERTRPKPKVKFDVGERVKIIDGP
                     FSNFLGNIDSIDADRGTLKVMVEIFERLTSVEVEFWQVEKI"
     gene            complement(4627..4851)
                     /gene="secE"
                     /locus_tag="PKW60_08710"
     CDS             complement(4627..4851)
                     /gene="secE"
                     /locus_tag="PKW60_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPJ99551.1"
                     /translation="MAKESAVEKPGISARAREFFHEVRVEMRKVTWPAKDEVRSSTSV
                     VLFMLVLMAFIIGVFDKLSDVFVWLLLKLG"
     gene            complement(4902..4978)
                     /locus_tag="PKW60_08715"
     tRNA            complement(4902..4978)
                     /locus_tag="PKW60_08715"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4942..4944),aa:Trp,seq:cca)
BASE COUNT          937 a         1664 c         1446 g         1085 t
ORIGIN      
        1 ctcgtcgtcg ccaatgccga ccgcaaaccc agcaccttca cgatcgcctg gcggggtaaa
       61 tcgttcgcgg cggaagtccc cgcgcgcgcc accgccacct ttgtgtggta cgcggacgaa
      121 atgggacagc cggagactgt ctcgaatgtc tcgagcgggc aggcggatcc atgagcgccg
      181 cgaacgcggt caggggccat tcgccggctg cgatttgaga aactgcacgg tgtaggacgt
      241 ttccgacagc gggctgaaga tataaccctt cgaccgcaac tcccggatga tgacaggcac
      301 ggcttccacg gtcgcggcgt gcgtgtagct gtcgtgcatc aggacgattg cctggtcgcg
      361 tttcaggacg ccttcaatca cggttttggc gatggcctca gagctgctgg cgttcacgcc
      421 atgatccagc gagtcgacgt tccagtcgac gtgccggtag cccagtttcg ccatccgatc
      481 cacaagctcg tgcgtgatgc tctttccgtc gggcccccgg gtgatcctgt tattggaccc
      541 ggcgggaaag cgcagcaggc gcggccgggt tcccgtgaac cggaagatca aatcgtccag
      601 ccgggccaca ttcttctcaa acgcatcggc cgatttgtac acggtcctgt attggtgtga
      661 aaaggtgtgg tttcccagtg cgtgcccttc atcgagaatc cggttgtaca tccggttccc
      721 gaattcggtc acgttgccgc agacgaaaaa cgttgccgcc gcctcgtgct gcttgagaac
      781 atccaggagg gcaggtgtga gctctgacgg gccatcgtca aacgtcagat ataccgtctt
      841 tacccccggt gggggcgcgg atgccgagtc cgccgccggc agggctgagg aatcggcgca
      901 cacggcccgg gtgcataaaa acacggcgca gagcagtagc ggaaagacaa tccagcggct
      961 ggcaggcatg tggcaaccct cttggaaaat gggcgcggca tcataagaaa ccgcggggaa
     1021 cgcccttttg tttcgagcgc tccccgcggc caatcatacc acagcgatca gatgcctgcc
     1081 cgttgtcgca gagcctccac gtgaccggtg ttttcccagc gcaatcccgc gttttcccgg
     1141 ccgaaatgcc cgtaattggt cgttttgcgg aagattggcg tgcgcagatc aagcagctcg
     1201 agaatcgcag cgggacggca gtcaaactcc tccgcaagca gtgtggacag cgcgtcgtcg
     1261 ctcagcgcgc cggtgccgaa cgtggtgacg tgaatggata cgggacgcgc cacgccaatg
     1321 gcgtaggcca gctgcacttc acaccgcgag gccaatcccg cggccaccac gtttttcgcc
     1381 atgtagcgag ccatgtaggc gcccgaacgg tcaactttcg acggatcctt tccgctgaac
     1441 gcaccgccgc cgtgccggcc catgccgccg taggtgtcca caatgatttt tcggcctgtc
     1501 aggccgcaat cgcccgccgg ccctcccacc acgaagcgcc cggtgggatt cacatggtag
     1561 atgatatcgc cgttgaccag ctctttcggc acgaccggct cgcagacctg gcgaatcacc
     1621 gcgtcgcgga tgtcctgctg tgaaatatcc ggcgagtgct ggttggagat tacaatcgtg
     1681 tgaatgcggt gcggtttgcc gttgcggtat tccacagtaa cctgcgactt gccgtccggc
     1741 tgcagccacg gcagcgatcc gttgtgccgt aactcggaca gcttcaaacc aagtttgtga
     1801 gccagcgcga tgggcagcgg catgtattcc ggcgtttcat cgcaggcgta tccgaacatc
     1861 atgccctgat cgcccgcgcc ctgttccttg ttggtggcgg catccacgcc ctgggcaatg
     1921 tcaggagact gcttgtccag cgacaccaga acgccgcagc tgcgcccgtc aaatcccgaa
     1981 tcgccgccgg catagccgat cgccgcaatg gcctcacgaa ccaccttcgg ataatcggcc
     2041 acagccttgg tggtgatttc gcccgcaatt acacagaacc ccgtcgtcac catcgtttcg
     2101 cacgccacgc ggctcatcgg atcctgttcg atgacggcgt caaggattga atcggagatg
     2161 aaatccgcga gtttatccgg atgtccctcg gtcacagatt cggatgtgaa caagacccct
     2221 ttggtctgct ggctcattgc ggcctccttc tgctgcctgc aagacttcgc ctccggtcgc
     2281 gcacccggcc cgcacacagg ccgggccccc cgtcaaaaga aaacgggcgc cgcaaccttg
     2341 aaaaccaggg tgctgcgccc gaatgtaccg tccatatcgt ccacaaaatc atcacgcgcg
     2401 aaactggctg accaggctcg gcgcgtccag gcgaaagcca ggccccatcg tgctgctcat
     2461 gcagcagctc ttgagatacg tgcctttgca tgccgctgga cgggctttca ccacggcctc
     2521 aacgaccgcc gccgcgtttt cccggatctg ctcgaccgcg aaggaagctt ttccgatggg
     2581 gacatggata ttggcatttt tgtccacgcg aaaatcgatt ttacccttca ggatttcggt
     2641 cacggcctcg cccaccttcg cggtgacggt accggccttc gggctgggca tcagcccgcg
     2701 cggacccagg actttgccga gcttgcccac gtcacgcatc atgtccggcg tcgagacggc
     2761 cacgtcaaaa tcggtccacc ccccctgtac tttctggagg agctcttcgg cgcccgcgtc
     2821 gatcgcgccg gcctcgcggg ccgcgttgat ctgttcggtt tctttacaga acaccaccac
     2881 acgcacctgt tttccggtgc cgtggggcaa agagacagtg ccgcgcacca tctggtcggc
     2941 gtggcgcggg tctacgccca gcgtaaacgc cagttcgacc gtttcatcga acttcgcggt
     3001 ggcacaggct ttgaccccgg tcgctgcttc gtccagcgta cacgcaatct gcttgaggtt
     3061 ttgctcgttg agagcttttc tccgtttcga ttggctcgac atggcgtgat ctccttgtgg
     3121 tcggcgggcg caaggccctc ccacagatgg tattgtctat acccagcgcc tcctgcggag
     3181 gcgaactgct cagttttcga ccagaatgcc catgctccgg gccgtgcccg cgagcatccg
     3241 caccgcggca tcggtatccg ccgcgttcag gtcgtctttc ttgatttccg cgatttcgac
     3301 cagttccgcc ttgcgcaccg agcccacctt gtttcggttg ggttccccgc tggctttcgc
     3361 gagcttggcc gcgcgcttta tcagcacggc cgccggcgga ctcttcgtaa taaacgaaaa
     3421 actccggtcc tcatagaccg tgatcacggc cggaatgacc aggccctgct ggtctttcgt
     3481 gcgctcattg aactgcttgc agaaatccat gatgttgacg ccgtgctggc ccagcgccgg
     3541 gcccaccggc ggcgccggat tggcctggcc cgcgggacac tgcagcttga tcagcgccgc
     3601 tacattcttg cccttcttac ctttcgccat cgcaccgtct tctccgtatg ttaaccagga
     3661 ttagatcttt tcaacctgcc agaattcaac ttcgacgctg gtgagccgct cgaaaatttc
     3721 caccatgacc ttgagggtcc cgcgatccgc gtcgatcgaa tcgatattcc caagaaaatt
     3781 agagaatggc ccgtctatga ttttgacccg ctcgcccaca tcaaacttga ccttcggctt
     3841 gggccgcgtc cgttcgccgc gaatctcgtc aatgatggca ttcacttccg cgtcttccag
     3901 cggtacgggc gtgtgccgcg agccgataaa cccgctcaca cccggcgtgt tattaatcag
     3961 atgccacagt tccggccgcc gttcgggatg ttctggaagc cgcaccagca cgtagccggg
     4021 gaaaaacttg cgcttcgaga tccgtttctc gccgcccttg atctcggcca cctgttccat
     4081 gggaaccagc acgccgccga tcaggtcatc cagcccgtcg agttccgcct gcatcagcag
     4141 attcttgcgc acgttgcctt cctggcccga ataggcgtgc aaggcatacc accgccgccg
     4201 gacgccgtcg tccggatccg gtttgagggt tgccaggatg gggtcttctt tcggcgcttc
     4261 gtcgtcgccc tcgtcattgc tgtccgcacc cgtgcccagg tcgcggactt cgacttcgac
     4321 ggatttcgcc ggagtgccgc cgccatcgcc cgccagaaac cggcgtgcct gtaattccgc
     4381 ctcggcctcc tgggcttcct gagcctcttg ttcccggcgg agatcctccg tgagcgcctt
     4441 gcgggcttct gcttcagcgg cgtcttcttc ctgggaagac tcctttttca cgtcgccaaa
     4501 aaagctggcc gcaaactcgc gttcagccgc gtctttcgcg gcgcgttcgg actggcgttc
     4561 gcgaagctcc tcgtcgagct tttgaggttt cttgtcgttc tgttcgtctg tcacaacggg
     4621 agactcctac ccgagtttaa gcagcaacca aacgaacaca tcagaaagct tgtcgaacac
     4681 gccgatgatg aacgccatga gaaccagcat gaaaagcacc acggaagtcg acgagcggac
     4741 ttcatctttg gccggccagg tcaccttgcg catctccacc ctgacctcgt gaaaaaattc
     4801 acgggcgcga gctgaaattc ccggtttttc tactgctgat tccttggcca tactcgctcc
     4861 tgctgcgacc gttccaagct ccgttcatgc gtacacaaac atggcaggac tggagggact
     4921 cgaaccccca accttcggct ttggaggccg ctgctctagc cgattgagct acagtcctgc
     4981 ataaaagcgg gccgtgaccc gctttaaaga caatcttgca gagtatagac ggtttgacgc
     5041 cacggttcaa aaaccgcgcg ggatcaacac gtccgcctat ttaataatct tggttacgac
     5101 gccggcgccg acggtccgtc cgccttcgcg ga
//
LOCUS       DAOPBQ010000241         5097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13867_length_5097_cov_4.185442, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000241 DAOPBQ010000000
VERSION     DAOPBQ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13867_length_5097_cov_4.185442"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PKW60_08720"
     CDS             complement(<1..1239)
                     /locus_tag="PKW60_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPJ99552.1"
                     /translation="MLTIEVNNRKIEAKSGEMLLDALKRNGITVPTLCNMDNLFPTGA
                     CRMCVVEVEGERALVPSCAYPVRDGMRVQTHSPRALRARKTIVELLLANHPDDCLYCV
                     RSGNCDLQGMANELGVRERRYSGGKNEYHVDSSNPAIVRDPAKCILCGKCVRICEEVQ
                     GVSAIDFVGRGSQTVIGTAFSEGLNVSSCINCGQCIMVCPTGALREQSYIKEVLNAIA
                     DPTKLVVVQHAPSVSVTLGEEFGLKPGADVDGLLVAALRRLGFDRVFDTSFSADLTIM
                     EEASELAHRIQTGGKLPMMTSCSPGWIKFVEEFYPEMMDNLSTCKSPQQMMGAVIKSF
                     FAEREGVAPEDIFSVAIMPCVAKKFEAGRPEHSRNGTPDVDAVLTTRELARVIRMRGM
                     DMQSLAPEHADTPFGERSTAG"
     gene            complement(1270..3366)
                     /locus_tag="PKW60_08725"
     CDS             complement(1270..3366)
                     /locus_tag="PKW60_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPJ99553.1"
                     /translation="MNKETTAMKPPASLNGLLEYACGLTPDLDEAARAGYEEQLRLLR
                     RERTERPTIFVGAGTCGLGAGAGKSLNAIRAFLRLRNIDAEIVEVGCIGLCAEEPLVD
                     IQLPGKPRVSFKHVTEDKVKPLLESVLAETIPTDMVLGQFTTPGVAPWHNVDRLAEHP
                     FFAPQTRWVLKNCGVINPSSLGEYVAGGGYRALTKVIHTMTPAEVCDMVEQSGLRGRG
                     GGGFPTGKKWKFALSTTSDRKYLVCNADEGDPGAFMDRAVIEGDPHRLLEGMAIAAYA
                     IGASKAYIYIRAEYPLAIKRLQHAIAEAKDCGFLGENIQDSGFSLEIVIKMGAGAFVC
                     GEETALINSIEGKRGMPRPRPPFPAVKGLFDKPTIINNVETLANIPRIAEGGAEAFAA
                     IGSETSKGTKVFALSGKIALTGLVEVAMGTRLRQVLFDIGGGIPNGKKFKAVQIGGPS
                     GGCIPDEHLDIAIDYESLKTVGAMMGSGGLVVMDEDTCMVDVAKFFMEFIQRESCGKC
                     IPCREGTLRMLQILERITRGRKSESKFQALERFKGVMMLNRTAEVIRDTSLCGLGQTA
                     PNPVLSTLRWFRDEYEAHIYERRCPAGVCQELRSFEIDVELCDGCTLCAQRCPVDGAI
                     VGVPKKVHYIIEDKCIGCGTCVDVCPTHAARLRIPGAEEIDRERRESEQALSVFASGA
                     FEDWHEGAALYRMGKQ"
     gene            complement(3359..3859)
                     /gene="nuoE"
                     /locus_tag="PKW60_08730"
     CDS             complement(3359..3859)
                     /gene="nuoE"
                     /locus_tag="PKW60_08730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPJ99554.1"
                     /translation="MSTTTGQPAVQPDIQEILDRHADAGRDALIPLLQDVQETHGYLS
                     REAIARIGEHLHLPASKIYGVATFYNQFRFQPLGRYHIQVCRGTACHVKGSLGVLDAI
                     RQDLALEPGKTTRDGLFSLEVVACIGACGLAPVISINGEFYANLTPQKVRDIMDELRE
                     EVENDE"
     gene            complement(3859..4494)
                     /locus_tag="PKW60_08735"
     CDS             complement(3859..4494)
                     /locus_tag="PKW60_08735"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPJ99555.1"
                     /translation="MISDKTVARLSVYRRVLLAMQDAGQQHVFSHELAARSGCTAAQV
                     RRDVMATGYTGSPVRGYDVAALAGSINDFLFHSDAQKVALVGIGNLGRALMAFSPRRN
                     PRIPLVAAFDSDETKVNRVIHGCRCYPMQQLEEVVESEGIRVGIIAVPSSAAQDTARR
                     LCRAGVSGLLNFAPTHIWVPPHVYIENLDLSMALEKVAFFAGLRASQKETM"
     gene            4791..>5097
                     /locus_tag="PKW60_08740"
     CDS             4791..>5097
                     /locus_tag="PKW60_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99556.1"
                     /translation="MRIFTIVVSLLGVFFTMMAWSAEPEFAGNWANEAAVAEVAAGTR
                     TEANAAWWGFDPEDATAAMQAAIDSNAPRVRIPYMGRPWIVTPIRLRDNLELHFEPGV
                     "
BASE COUNT          901 a         1709 c         1472 g         1015 t
ORIGIN      
        1 gcccgccgtg ctgcgttcgc caaacggcgt atctgcgtgt tcgggggcga gcgattgcat
       61 gtccatgccg cgcatgcgga tgacccgcgc cagctcccgc gtggtcagga ccgcgtcgac
      121 atcgggcgtg ccgttccggg agtgttccgg ccgtcccgct tcgaattttt tggccacgca
      181 gggcatgatc gccacgctga agatatcttc gggcgccacg ccttcccgct ccgcgaaaaa
      241 cgacttgatc acggcaccca tcatctgctg cgggctcttg caggtggaca ggttgtccat
      301 catttcgggg tagaattctt ccacgaattt gatccagccc ggcgaacaac tggtcatcat
      361 gggcagcttc ccgccggtct ggatgcgatg agccagctcc gacgcctcct ccatgatcgt
      421 caggtcggcc gaaaacgacg tatcgaacac ccggtcaaag ccgagccggc gcaacgccgc
      481 caccagcaaa ccgtccacgt ccgcgcccgg tttcaagccg aactcctcgc ccagggtcac
      541 cgatacgctc ggcgcatgct gcaccaccac cagtttcgtg gggtctgcga tggcattcag
      601 cacttccttg atataactct gctcgcgcag ggcgcctgtc gggcagacca tgatgcactg
      661 gccgcagttg atgcagctcg agacgttgag cccttcgctg aaggccgtcc cgatcacggt
      721 ctggctgccg cggcccacaa aatcaatcgc cgagactccc tgcacttcct cacagatccg
      781 cacgcatttc ccgcagagga tgcatttggc cggatcgcga acgatggccg ggttcgacga
      841 atccacgtgg tattcgttct tgccgcccga gtagcgccgt tcccgcaccc cgagttcatt
      901 ggccattccc tgcaggtcgc aattgccgct gcgcacgcaa tacaggcaat cgtcgggatg
      961 gtttgcgagc agcagttcga cgatcgtctt gcgtgcccgc agcgcccggg gcgaatgcgt
     1021 ctggacccgc atcccgtccc gtaccgggta ggcgcagctc ggcaccagcg cccgctcgcc
     1081 ctcaacctca accacgcaca tgcggcaggc gcccgtcggg aacaggttgt ccatgttaca
     1141 cagggtcggc actgtgatgc cgttccgttt cagcgcatcc agcagcatct cgccgctctt
     1201 cgcctcaatc ttccggttat tgacttcgat ggtaagcatt gccgttcctc gttcgagcgc
     1261 gtccacgcgc tattgtttgc ccatacggta aagcgccgcg ccctcgtgcc agtcttcgaa
     1321 ggcgcccgat gcaaacacgc tcaaggcctg ctcgctctcg cggcgttcac ggtcgatctc
     1381 ttccgcgccc ggaatccgaa gccgtgcggc gtgtgtcggg cacacatcca cacaggttcc
     1441 gcaaccgatg cacttgtcct caatgatgta atgcaccttt ttcggcacgc ccacgatcgc
     1501 cccgtccacg ggacagcgct gcgcgcacag agtgcagccg tcgcacaatt ccacgtcgat
     1561 ctcgaaggag cgcagctcct ggcagacccc cgcgggacaa cgccgctcgt agatgtgggc
     1621 ttcatattcg tcgcggaacc agcgcagcgt gctcaacacc ggattcgggg ccgtctgccc
     1681 caggccgcac aggctcgtgt cgcgaatgac ttcggccgtc cggttgagca tcatgacgcc
     1741 tttgaagcgc tcgagcgcct ggaatttcga ttcgcttttg cgaccccgcg tgatgcgttc
     1801 gagtatctgg agcatacgca gcgtgccttc ccggcagggg atgcacttcc cgcagctttc
     1861 ccgctggatg aactccatga agaacttggc cacgtcgacc atgcacgtgt cttcgtccat
     1921 caccaccagg ccgccggagc ccatcatcgc gcccacggtc ttcagcgatt cgtagtcgat
     1981 agcgatgtcc agatgctcat cggggatgca tccgccggac gggccgccga tctgcaccgc
     2041 cttgaatttc tttccgttcg ggatcccgcc gccgatatcg aacagcacct ggcgcagccg
     2101 ggtgcccatc gccacttcga ccaggccggt cagcgcgatc ttgcccgaga gcgcaaacac
     2161 cttggtgcct ttgctcgtct cggagcctat cgccgcgaac gcctccgctc ctccttcggc
     2221 aatccgcggg atattcgcca gggtctccac gttgttgata atcgtcggct tatcaaagag
     2281 gcccttgacg gccggaaacg gcggacgggg acggggcatg ccccgctttc cttcgatgct
     2341 gttgatcagt gcggtctctt cgccacagac gaaggcgccc gcgcccatct tgatcacgat
     2401 ctcgaggctg aatccgctgt cctgaatgtt ttcgccgagg aagccgcagt ctttcgcctc
     2461 ggcaattgcg tgctggagcc gtttgatggc cagcggatac tcggcccgga tataaatgta
     2521 ggccttgctt gcgccgatgg cgtaggctgc aatggccatg ccctccagca gccggtgcgg
     2581 atcgccttca atcaccgccc ggtccatgaa cgcgcccgga tcgccttcgt ccgcgttgca
     2641 gaccaggtat ttccggtcgc tggtcgtgct cagcgcgaat ttccactttt tgcccgttgg
     2701 aaagccgccg ccgccccggc cgcgcagccc gctctgctcg accatgtcgc agacctcggc
     2761 cggcgtcatg gtgtgaataa ccttcgtcag ggcccggtag cccccgcccg cgacgtattc
     2821 tcccagactc gatgggttga tgaccccgca gttcttcagc acccaccgcg tctgcggcgc
     2881 gaagaaggga tgttccgcaa gccggtcgac gttatgccag ggcgccacgc ccggcgtggt
     2941 gaactggccc agcaccatgt ccgtggggat cgtctccgcc agcacgcttt ccaataaggg
     3001 cttgaccttg tcttccgtca cgtgcttgaa cgagacgcgc ggcttgcccg gcagctgaat
     3061 gtccaccagg ggctcttcgg cgcacaaccc aatgcatccc acttcgacaa tctcggcgtc
     3121 aatgttgcgc aggcgcaaga acgcccggat cgcgttgagc gatttgcccg cgcccgcgcc
     3181 caggccgcag gtgcccgcgc ccacgaagat cgtcgggcgc tcggtccgct cgcggcgcag
     3241 cagccgcagc tgttcctcat agcctgcacg cgcggcctcg tcgaggtccg gcgtgagacc
     3301 gcatgcatac tctaacaacc cgttcaggct ggccggcggt ttcatcgctg ttgtttcctt
     3361 attcatcgtt ttcgacctct tcgcggagtt catccataat gtcacggacc ttttgagggg
     3421 tcagatttgc gtaaaactcg ccgttgatgc tgatcacagg cgccagaccg caggcgccga
     3481 tgcacgccac aacctccagg ctgaacaggc cgtcgcgcgt cgtctttcca ggttcgagcg
     3541 ccagatcctg ccggatcgca tccaggacgc ccagcgaacc ttttacgtgg catgcggtgc
     3601 cgcggcagac ttgaatgtga taacgcccga gcggctgaaa acggaactgg ttgtagaagg
     3661 tcgccacgcc gtaaatcttg ctcgccggaa gatggaggtg ttcgccgata cgcgcgatcg
     3721 cctcccgtga caggtagccg tgtgtctcct gaacgtcctg aagcaggggg atcagcgcgt
     3781 cccgccccgc atccgcgtgc cggtcaagaa tttcctggat atccggctgc acggccggtt
     3841 gtcccgtagt ggtgctcatt acatggtttc cttctgcgac gcccgaaggc ccgcaaaaaa
     3901 cgcaaccttc tccagcgcca tgctcaaatc cagattctca atgtatacat gcggcggaac
     3961 ccagatgtgc gtgggggcaa aattcagcag gcccgacaca cccgcacggc acagccgccg
     4021 tgcggtgtcc tgggccgcgg acgacggcac cgcgatgatg cccacccgga tcccttccga
     4081 ctccacaacc tcttccaact gctgcatcgg ataacaccgg cacccgtgga tcacccggtt
     4141 cactttcgtc tcgtcgctgt cgaaagccgc caccagaggg atacgcggat tgcgccgcgg
     4201 cgaaaacgcc atcagcgcgc ggcccaggtt gccgatgccc accaacgcca ccttctgcgc
     4261 gtcggaatga aacaaaaagt cgttaatgct tcccgccaga gccgccacgt cgtacccgcg
     4321 aaccgggctg ccggtataac ccgtggccat cacgtcgcgc cggacctggg ccgccgtgca
     4381 tcccgaccgc gcggccagct cgtgtgaaaa cacatgctgc tgcccggcgt cctgcatcgc
     4441 cagcaagacc cgccggtaca cactgagccg ggccacggtt ttatcggaaa tcacggcaat
     4501 tcctcgctgt ttgcggaaga actgtgaaga cattcacgtt gtgattcgtt tcacagagac
     4561 tataccaccc ggcgaggcct catgtcaaga aaaatcgtga cgttttgtca cgattggacc
     4621 tcagcttcta cgcccttact ggcccgagat ttaaggccgg gcttgcactt ccgttcggga
     4681 tcgggcatgc tgaattctgc gcgaacagtg cctccgcggg ttaaatgcgt gttgatctgc
     4741 gtcttttttt ggggccagag gcactgtaca agccaaatgg aggagaaacc gtgcgtattt
     4801 tcacgattgt cgtgtcgctt ctgggagtgt ttttcacgat gatggcctgg tccgcggagc
     4861 cggaattcgc gggcaactgg gcgaatgaag cggcggtagc ggaggtggcc gctggcaccc
     4921 gcacggaggc caatgccgcc tggtggggct ttgatcccga ggatgcgacc gccgcgatgc
     4981 aggcggcgat cgattccaat gccccgcgcg tccgcatccc gtacatgggc cgcccgtgga
     5041 ttgtcacgcc catccgcctg cgagacaacc tggaactgca tttcgagccg ggagtgg
//
LOCUS       DAOPBQ010000242         5092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13882_length_5092_cov_3.574548, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000242 DAOPBQ010000000
VERSION     DAOPBQ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13882_length_5092_cov_3.574548"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="PKW60_08745"
     CDS             <1..1211
                     /locus_tag="PKW60_08745"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPJ99557.1"
                     /translation="PVEWYNRRDEVVPQIEVRGLNLIFGGHTMHTWVSPEHFEEHPEW
                     FAYIDGERKAPTLCVTNAAMEQELVKNMRAFIDRCPEVDVVDLWHPDGQAFCHCPVCT
                     RGLLPESTQGKLPEGMPADVVQSAYVITYIEFMNRVAAEIGQSHPDVMISPLIYGPSD
                     RAMPDGCPALADNLMPGLAHIFRDSYRPLAGEPKSALNMRFLGNDVTWMAKSQDSYIY
                     EYYNCWIPPFIYPGSRVIARDLQILNEVGAQGSSSDMYGYSPINMYVAARALWDPQAD
                     WAEMVRDYHQRYFIDVASEMAQNWIDLENGIYGMQGYQGNGAMDTKFDWMSDECGLYL
                     KSRRTDQIAFHEQMIAQTDIPLVRTRLERSLKPWKVWGMEAQWWAFPEFEGEGPRAPA
                     VIPLPPGMEQ"
     gene            complement(1315..1596)
                     /locus_tag="PKW60_08750"
     CDS             complement(1315..1596)
                     /locus_tag="PKW60_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99558.1"
                     /translation="MTRAWQPQERRPTAPSTARLVQALPQTVWAPWCEEQRRTATLHL
                     PFDLREGITQRPGLTAGQTSETRSLERELVPGRLHIADRATATMVWSTF"
     gene            complement(2146..3411)
                     /locus_tag="PKW60_08755"
     CDS             complement(2146..3411)
                     /locus_tag="PKW60_08755"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPJ99559.1"
                     /translation="MSGNIREAKPMSLQGVGGHAEQRVPQAWRFNGNERKYIEEVMAR
                     GLLSSRHGLMSQRFEHAFAEKFGVKYAITVNSGTSTLHSAVACAGVGQNDEVIVPAFT
                     IICSAAAIIHHNGTPVFADIDRRTFNIDPEDVRRKITERTKAIMPVHINGLCCDMDPI
                     LKLAEEHNLLVIEDSAQTFLGRDDKERIAGTIGHCGSFSFEQTKHMTTGDGGLLVTND
                     PDLARRLRQVGEHGARCVSPESSVWQGPLIHDYIGWNYRFPELCAAVGLAQLERLDEF
                     VAYRTKWAMEYQQVISQVPWLVPQYIPPGYLHSYYAYMFTFEGEQYGVRREDWDNALA
                     AEQFEGGPFARVIYYEPGLAAMRCYGQDGFARSIPPDANPSPDSPYAGSCPVAEEVQA
                     RSYWVHLFFDSAYLEKNIEKLARAVKRFV"
     gene            complement(3493..5004)
                     /locus_tag="PKW60_08760"
     CDS             complement(3493..5004)
                     /locus_tag="PKW60_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99560.1"
                     /translation="MPEGEAAKLAAWVKAGGRLVLDGYCGRNQRVLGPLVGIEGPLGR
                     AVHLPSYEDPSRIAPRIRVTNRSRIFAGMEPKRRSKVWGQVWFPEEGTRVTARPLAWI
                     DSPQGEKIGPGIYHHEVGRGEVYTWAFSSAYSQLLLMQGRGTTEDLGSFPARVAPGAT
                     RDGDVNTWGDQVVTDEEDQYFPSADYLLYPLLGILRQSVPDHMLISAVPEGRECGVIF
                     TGDSDRASVELVNRYTDMLSAHGIRPTQFICREGYEAKQLRGQCEYGIHPLFHETEQE
                     CFENLLSYGFAPDQLVCGRRHCLIHYGLTETLERMAKAGIRYTSNNWDFPYPETQSAA
                     FLFGTTHAHHIYNWEGKRIGIVNIPQVFMDYPPVMECTQASWRDTRRTHGVGAWNYHP
                     QNHVMPFWCEAIEWLAQQAQEAAWCGTMGEYGDWYLLRDRMHVSYRAGQVTIDGSLPK
                     GLTLLSRRNTLFVNGVRSRASATAVWHGVKYWVHVHDERTSTPSGRAGPANME"
BASE COUNT         1044 a         1528 c         1482 g         1038 t
ORIGIN      
        1 aaccggtgga gtggtacaat cggcgcgacg aggttgtccc ccagattgaa gttcggggac
       61 tgaatctgat attcggcggc cacacgatgc atacctgggt ctcgcccgag cacttcgagg
      121 aacaccccga gtggttcgcc tacattgacg gcgagagaaa agcgcccacg ttgtgcgtca
      181 ccaatgccgc gatggaacag gaactggtca agaacatgcg tgctttcatt gaccgctgcc
      241 ccgaggtcga cgttgtggat ttgtggcatc ccgacgggca ggcattctgt cattgcccgg
      301 tctgcacgcg cgggctgttg ccggaatcga ctcagggcaa gctgcccgag ggcatgccgg
      361 ccgacgtggt gcaatcagcc tacgtgatca catatattga attcatgaac cgcgttgcgg
      421 ccgagatcgg ccagagtcac cccgacgtga tgatcagccc gctcatctac ggcccgtcgg
      481 atcgggcgat gcccgacggg tgcccggccc tggccgacaa cctgatgcca gggctggctc
      541 atattttccg cgacagctat cggccgctgg ccggcgaacc caaatccgca ttgaacatgc
      601 gctttctggg caatgacgtc acctggatgg ccaagtcgca agacagctat atctatgaat
      661 actacaactg ctggatcccg cccttcatct acccaggatc gcgcgtcatc gcccgtgacc
      721 tgcaaatcct aaacgaagtg ggcgcgcagg ggtcatcctc ggatatgtac ggttacagtc
      781 cgatcaacat gtatgtagcg gcgcgcgcgc tctgggatcc ccaggccgat tgggcggaga
      841 tggtgcgcga ttatcatcag cgctacttta tcgatgtcgc ctccgaaatg gcccagaatt
      901 ggatcgactt ggaaaacggc atctacggca tgcaggggta ccagggcaat ggcgcgatgg
      961 acaccaagtt tgattggatg tcggacgagt gcggtctgta tctgaaatca agacggacgg
     1021 accagatcgc gttccatgag caaatgattg cgcaaacgga catcccattg gtccggacgc
     1081 ggctggaacg atccctcaag ccgtggaagg tttggggaat ggaggcgcag tggtgggcct
     1141 ttccggagtt cgaaggggaa ggaccccgcg cgccagccgt cattcctttg ccgccgggca
     1201 tggagcaata aggaacaact tgccgtctct ttgacccgca attaaggact gcgcaagaat
     1261 accttgaaca aacgatgttg tcgtgagcgc gggtgacgaa ggaactctcg gccttcagaa
     1321 tgttgaccag accatagtcg cagtagcacg gtcggcaatg tggaggcggc cgggaaccag
     1381 ctcccgttcc agactgcggg tctcgctggt ctggcccgcc gtgagcccgg gccgctgggt
     1441 gatgccttcg cgcagatcga atggcaggtg gagcgtggcc gtgcggcgtt gctcctcgca
     1501 ccacggcgcc cagaccgtct ggggcagggc ttggaccagc ctggccgtgg atggggccgt
     1561 gggccgccgc tcctgcggct gccaggcgcg tgtcacacgg cgtgtcatcg atccgctggc
     1621 cgaatctgct cagtgaaggt cacgatcaac tcgggatcaa agacctgagc agccttgaag
     1681 aagctgcccg ggctgaagcg gggcaggtcc agctcgcgcc gcggacggtc tcgatgctgc
     1741 tggcccgctg caggcgcagc atccaacatg aaggagcttg gcgtatggga gcagggcagg
     1801 gctcatggag cggcgcgaac aagtcgaggc ggcccgagac gagcttcgcc tcctgaacct
     1861 cgcgggcgca aatcccagct gcgggcatgc gaccgggttt gttgcgcgcc tgctcttccc
     1921 tgcgcggcca aatcggattc actcatcggc ttgacataac gccgaaattc ccgaacggaa
     1981 acacccgtgg cccggtcgct aactccgtcg tccactacca cccgggcctc ggctggccgt
     2041 ccttcacgcg aaagttcgcc ccctattctc gggatcgctc ggattggtct tccgtcccta
     2101 aaacaccacc gacaatgaca ttcattcttt tcttctcctc ccggatcaga cgaaccgttt
     2161 gacggcgcgc gccagcttct cgatgttctt ctccaagtag gccgagtcaa agaacaagtg
     2221 tacccagtag ctgcgtgcct gaacctcttc ggcgacgggg caagagccgg cataggggct
     2281 gtcgggcgaa gggtttgcat ctggtggaat gctccgtgca aagccatcct ggccgtagca
     2341 tcgcatcgcg gccagtccag gttcgtagta gataacgcga gcgaaggggc caccctcaaa
     2401 ttgctccgcg gccagggcat tgtcccagtc ctcccgccgg acgccatact gctcgccttc
     2461 aaaagtaaac atgtaggcat aataggaatg gagataaccc ggcggtatgt actgaggcac
     2521 cagccagggc acttgggaaa tgacctgttg atattccatc gcccacttcg tgcggtatgc
     2581 gacaaattca tctaatcgct cgagctgggc tagacccacc gccgcgcaca actcgggaaa
     2641 gcggtaattc cagccgatgt aatcatgaat cagtggacct tgccagacac tggactcggg
     2701 agaaacacaa cgcgcgccgt gctcgccaac ctgacgcagc cgccgcgcca gatccgggtc
     2761 gttcgtgacc agcaaacctc catctcccgt tgtcatatgt tttgtttgtt cgaaactgaa
     2821 ggagccgcaa tgaccgattg tgcccgcaat ccgctccttg tcatcacgcc ccaaaaaagt
     2881 ctgggccgaa tcctcgatca caagcaggtt gtgctcttcg gccagtttca ggatcgggtc
     2941 catgtcgcag cacaggccat ttatatgaac cggcatgatt gccttggtcc gctccgtgat
     3001 cttgcgccgc acatcctcgg gatcgatgtt gaacgtgcgc cggtcgatgt cggcgaagac
     3061 tggggtacca ttgtggtgga taatggcggc ggcgctgcag attatggtga atgctgggac
     3121 aatcacctcg tcgttctgtc ccacccctgc acaggcgacc gccgaatgga gtgtcgaggt
     3181 gccggaattg acggtgatgg catacttgac gccgaatttc tcggcaaaag catgctcgaa
     3241 gcgctgactc attagcccgt gtcggctgct gaggagaccg cgcgccatga cttcttcaat
     3301 gtacttgcgt tcattaccat tgaaacgcca ggcttgcggt acacgctgtt cggcgtgccc
     3361 acccaccccc tgcagactca tcggtttggc ttcgcgaatg ttgccggaca tttccttctt
     3421 ctccttgtcg actgatatct tactgttcgt ctgatttgcg gtcggttgcc acaatccgta
     3481 tccggaatga aactattcca tgttcgcagg gccggctcgc ccagacggtg ttgatgtcct
     3541 ttcatcgtgg acatgaaccc agtacttgac cccgtgccac acggcagtgg ctgaagctcg
     3601 gctgcgaacg ccattgacga aaagtgtgtt gcgcctgctc aagagcgtca gccccttcgg
     3661 gagggaaccg tcgatcgtca cttgccccgc gcggtaacta acgtgcatgc gatcccgcag
     3721 caggtaccag tcgccatact cacccatcgt gccacaccag gccgcctcct gcgcctgctg
     3781 tgccaaccac tcgatcgcct cgcaccaaaa gggcatgaca tgattttgtg gatgataatt
     3841 ccacgcgccc accccatggg tccgtcgcgt gtcgcgccac gacgcttgcg tgcattccat
     3901 cacgggtggg tagtccataa agacctgggg aatgttgacg attccaatgc gcttgccttc
     3961 ccagttgtat atgtgatggg catgcgtggt gccaaacagg aaggccgccg attgagtttc
     4021 tgggtaaggg aaatcccaat tattcgaagt atagcggatg ccggccttcg ccatgcgctc
     4081 caaagtttcg gtcaatccgt aatgaatcaa acaatgtcgc cggccgcaga cgagctggtc
     4141 cggcgcaaag ccatacgaca atagattctc gaaacattcc tgttcagttt catgaaagag
     4201 aggatgaata ccgtattcgc attggccgcg aagctgtttc gcctcgtatc cttcgcggca
     4261 aatgaactgc gtgggacgga ttccatgggc tgacagcata tccgtgtagc gattgacgag
     4321 ctccacgctg gcacggtcgg aatctcccgt aaagataacc ccgcattccc gcccttccgg
     4381 cacggccgat atcaacatgt gatccggtac ggactggcgc aggatgccca atagggggta
     4441 aagcagatag tcggccgagg gaaaatattg atcttcctca tcggtgacca cctgatcgcc
     4501 ccaggtgttg acgtcgccat cgcgagtcgc tccaggcgct acgcgcgcag gaaagctgcc
     4561 caagtcttcg gttgtaccac gcccctgcat caacaggagt tggctgtagg ccgaggaaaa
     4621 ggcccaagtg taaacttccc cccgccccac ctcatgatga tagatccctg gaccgatctt
     4681 ttcgccttgg ggcgagtcaa tccaggcgag tggtctggcc gtaacgcgcg tcccttcctc
     4741 aggaaaccag acctgtcccc agactttgct gcgccgtttc ggttccatac ccgcaaaaat
     4801 gcgactgcga ttcgtcactc gaattctggg ggcaatacgc gaaggatcct cataagatgg
     4861 caggtggacc gcacgtccca acggcccttc gatgccgacg agtggaccga gcacgcgttg
     4921 gttccgcccg cagtagccat ccagaaccag tctgccaccc gccttcaccc atgccgccag
     4981 tttggccgcc tcgccctcgg gcataccggc cagactggcg gcatgggtga aggcgggtgg
     5041 cagactggtt ctagatggct actgcgggcg gaaccaacgc gtgctcggtc ca
//
LOCUS       DAOPBQ010000243         5080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13918_length_5080_cov_3.164378, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000243 DAOPBQ010000000
VERSION     DAOPBQ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13918_length_5080_cov_3.164378"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PKW60_08765"
     CDS             <1..870
                     /locus_tag="PKW60_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HPJ99561.1"
                     /translation="GGEGIKLAYNTAAESFLKDGVNRVILATDGDFNVGMTDTSQLIA
                     LVEKKAKTGVFLSVLGFGMGNLKDSTLEQLADKGNGNYAYIDDMDEARKVLVTDMLGT
                     LVCIAKDAKIQVEFNPAQVQAYRLIGYENRILAKEDFNDDTKDAGEIGAGHTVTALYE
                     LVPPGVSLEVPGVDPLKYQQPRPALTDAAGSGELFSLKLRYKKPDQDNSRLLTFPVRD
                     DGAACENASEDFRFAAAAAGFGMLLRNSEHRGALTYELVLTLAEQGLGRDEEHYRAEF
                     IGLVRKAHALASQ"
     gene            1193..2716
                     /locus_tag="PKW60_08770"
     CDS             1193..2716
                     /locus_tag="PKW60_08770"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99562.1"
                     /translation="MGKATVNAGEGWDQLERLILDLQNMSADGFRSGAEQITKAHDLL
                     ETVRTDFASRGKSAAHAVKQIMGAVQRLRKASTETGALRQTIAGLEEQLGLRVSELSA
                     QKEMSLQFEKRLSEQEHAFQAAKQRVAELERQATEAQHALESERTARAALQQQLDDTE
                     NAVNARNVDIAEGRKEVEGLKHGLAEKEQELLHLENRLQTLEKEKNETDVSRSTQRTE
                     LDKIQTELDKTRVELDAVRTNKKVSDAEIVSLKEKLENALAETAGLHERLSKASDAAG
                     RAAELEAALNAANERVNTLEARLKEEMADVARASSVAEQLAQAAKEQHAPRQELQPQQ
                     TGMTGGAAPEKPGAPAAPSFPETEGRRWKLGEILWEMGVITLEQLEDALEEQRLNPQA
                     KLGTILEEKGYASPEAVAQAVALRAETAYVEVDAQSIAPDVAGLIPARLASLHKCIPI
                     RADGDTLTLAMANALDIVAIEDVERISQRRVEPVVATLPRILELIDQLYERSPSARR"
     gene            complement(2789..>5080)
                     /locus_tag="PKW60_08775"
     CDS             complement(2789..>5080)
                     /locus_tag="PKW60_08775"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPJ99563.1"
                     /translation="QLLAEYVERATGARLPVTSALPDEGAVVCVGRGAAWQTPDQAGL
                     DEDGFDIAFPDASTVVIAGPTDWGTEFGVYEFLERYVGVRWLLPGEHGDDVPAARSLS
                     VPPEPLRQAPAIFSRLFSGLPGEAQTTWARRNRMHGRVSFHHYLLHLFPPETYTASHP
                     EFFPILNGKRFLPPDNNAHYWQPCFTAPGIVEEAVKNIIEYFETNPAATSYSLGANDS
                     SGYCQCERCLARLSGEKNFLGCVDYSDLYYDWANQVIEGVLERFPDKWFGCLAYSEVA
                     APPQHTSVHPRLIPYMTYDRMKWKDPELREAGEALTRDWNARSPLLGWYDYIYGSPFC
                     LPRVWFHHMADYYRFGYANGVRAMYAEAYANWGEGPKLYVSLKLQWDPNADVDALLDE
                     WYVRCVGGEAAPFLKQYYAHWEDFWTRRVLDSRWFTKQGQYLAFDDPGYLRDITREDI
                     EMSRRWLESAVEKAGTEPQQYRARLLLQAFEYYEASAYAFAFDAPLGEPATTQEQATA
                     LLAALEQGFKHAARRRELSLEVFPDHPVLRHPLTMTQYAALANDTMGAVSLGALIAFL
                     AAPDSPFRSELMRVAEQAPDAYLKEQASMMLELAEEKGELLTRNPSFEEGSGGAAEGW
                     SWWLRDGDGRMARSDAMAHSGGHAVVCEGVKRGGPVQVVPAQPGRYGLICHVFSPADQ
                     ESAGTVELSLTLRDARGINVLTPSAKQALTPGVWQPLAIVHRITEQDMANVVEILPIL
                     VVNGQKTGELLYFDDLRLYRLGD"
BASE COUNT         1047 a         1552 c         1610 g          871 t
ORIGIN      
        1 ggcggcgagg gcatcaagct cgcctacaat accgccgcgg agagcttcct caaagacggc
       61 gtcaaccggg tcatcctggc caccgacggc gacttcaacg tcggcatgac cgatacctca
      121 cagttgatcg ccctggtgga gaaaaaggcg aagaccggcg tcttcctgtc cgtgctcgga
      181 ttcggcatgg ggaatctcaa ggactccacg ctcgaacagc tcgccgacaa gggcaacggc
      241 aactacgcct acatcgacga tatggacgaa gcacgtaaag tgcttgtaac agacatgtta
      301 ggcaccttgg tctgtatcgc caaggatgcc aagatccagg tggaattcaa cccggcgcag
      361 gtccaggcct accgcttgat cggctacgaa aaccggatac tggccaagga agacttcaac
      421 gacgacacca aagacgccgg cgaaatcggg gcgggacata cggttaccgc gttgtatgag
      481 ctggttcctc cgggggtgtc cctcgaggtt ccgggcgtgg acccgctcaa gtaccagcag
      541 ccccggcccg ccctgacgga tgccgcgggc tccggcgagc tgttttcgtt gaaacttcgc
      601 tacaagaaac cggatcaaga caacagcagg ctgctgacgt tcccggtcag agacgacgga
      661 gccgcctgcg agaacgcgtc cgaggatttc cggttcgccg ccgccgccgc ggggttcggc
      721 atgctgctgc gaaactcgga acacaggggc gcgctcacgt atgagctggt cttgacgctg
      781 gctgaacagg gcctgggtag ggacgaagaa cactaccgcg ccgagttcat tggactggtg
      841 cggaaagcgc acgccctggc aagccagtga gcaggaatgg gccggggcag actctcatcc
      901 acctcggggt ctccccggcc ctcctttcgt ctaccgacgc gacaccctgt ctatagccct
      961 ccaaaccggc gctctttggg gtataatcaa ccggggactg atgcgccgcg gtaatgacgg
     1021 ctgtttttca gctatttggg ggagttttga gcggtatatt cggggcgagg gtcccgaaaa
     1081 ttgcattttt cgctgttttt tgtaacaaag tatggtataa cttcaagggg aaatctgcag
     1141 agggccgcat tcgtggaatg gggagttgct gggctgggga tggggaatac ctatggggaa
     1201 agcgacagta aacgccggcg aggggtggga tcagctcgaa cggttgatcc ttgatctgca
     1261 aaacatgtca gccgatgggt tccggtcggg ggcggaacag attaccaagg cccacgacct
     1321 gctggaaacc gtccggaccg atttcgcgag ccggggtaaa tctgcggcgc acgccgtgaa
     1381 acaaatcatg ggggcggtcc aacggcttcg aaaggcctcc acggaaaccg gcgccctccg
     1441 ccagactatc gcaggcctcg aagagcagct tggcctccgg gtcagcgagc tttccgcgca
     1501 gaaagagatg tccctgcaat ttgagaagcg gctatccgag caggagcatg cgtttcaggc
     1561 ggcgaagcag cgcgttgcag agctggaacg gcaggccacg gaggctcagc acgctctgga
     1621 atccgagcgc acggccagag ccgccctgca gcaacagctg gatgacaccg aaaacgccgt
     1681 gaacgcccgc aatgtcgaca ttgcagaggg caggaaagag gtcgaaggcc tcaaacacgg
     1741 cctcgccgaa aaagagcaag agctgctcca cctggaaaac cgcctccaga ccctggagaa
     1801 ggaaaagaac gagacggatg tctcgcgcag tactcaaaga accgaactgg acaagatcca
     1861 gaccgagctg gacaagaccc gggtcgaatt ggacgccgtg cggaccaaca aaaaggtgtc
     1921 cgacgccgaa attgtctcac tgaaagaaaa gctggaaaac gcgctcgctg aaaccgccgg
     1981 gttgcatgaa agactatcga aggcgtctga cgctgccggg cgggccgctg agctcgaggc
     2041 ggcactgaac gccgccaatg agcgcgtgaa caccctcgaa gcccgtctca aagaagaaat
     2101 ggccgacgtg gcccgggcct cctcggttgc ggagcagctc gctcaggccg ccaaagaaca
     2161 acatgcgccc cgacaggagc ttcagcccca gcagaccggc atgacgggag gcgcggcccc
     2221 ggaaaagccc ggcgctccgg ccgcgccctc tttccctgaa acggaggggc gccggtggaa
     2281 gctgggcgag atcctgtggg aaatgggcgt gatcacgctg gaacagctcg aggacgcgct
     2341 tgaagagcag cgcctgaacc cgcaggccaa actcgggacc attctggaag aaaaaggcta
     2401 cgcctcaccg gaggccgttg cccaggcggt ggctctgcgc gcggaaaccg cgtacgtcga
     2461 agtggacgcc cagtcgatcg cgcccgatgt ggccggcctg atcccggcgc gcctggcctc
     2521 tctgcacaaa tgcatcccca ttcgtgcgga cggagatacc ttgaccctcg ccatggccaa
     2581 cgcgctggac atcgtcgcca ttgaagacgt cgaacgcatc agccagcgcc gggtcgagcc
     2641 tgtcgtggcg accttgccgc gcattctgga gctgattgac cagttgtacg aacgcagccc
     2701 gtcggcacgc cggtaacccg ggaagcgccg ggactggcgt tgcccggggt ggccggtgag
     2761 cgatcgctgc gtacgttgct cctgcggttt aatctcccag ccggtagagc cgcaaatcgt
     2821 cgaaatacag cagttctccg gttttttgcc cgttcaccac cagaatgggc agaatttcga
     2881 ccacgttggc catgtcctgt tcggtgatgc gatgcactat cgcgaggggc tgccacacgc
     2941 cgggagtcag ggcctgtttt gcagacggcg tcagcacgtt aatgccgcgt gcatcacgta
     3001 aggtcaacga caactccacc gtgcccgctg attcctgatc cgcgggtgag aagacgtggc
     3061 agatgaggcc gtagcggccg ggttgggcag gaaccacctg cacggggccg ccccgtttca
     3121 caccctcaca gacgacggcg tggcctcccg aatgggccat ggcatcggaa cgggccatgc
     3181 ggccgtctcc gtcccgcagc caccaggacc agccttcggc ggcgccgccg gaaccctctt
     3241 caaacgacgg gtttcgcgtc agcagctcgc ccttttcctc ggccagctcg agcatcatcg
     3301 atgcctgctc tttcaggtat gcatccggcg cttgctctgc cacgcgcatc aattcgctcc
     3361 gaaacggact gtccggcgca gcgagaaacg cgatgagggc gcccaggctc acggcaccca
     3421 tggtgtcgtt tgccagcgct gcgtattgag tcatcgtgag cggatgccgc agcacgggat
     3481 ggtcggggaa gacctccagg gaaagttccc ggcgccgcgc ggcatgcttg aatccctgct
     3541 ccagggcggc gagaagcgcc gtagcctgct cctgcgtggt ggccggttcg ccgagcggcg
     3601 catcgaaggc aaacgcatac gccgacgcct cataatactc aaatgcctgc aggaggagcc
     3661 gcgcccggta ttgctgcggc tccgtgccgg ctttctcgac agcagactcg agccagcgcc
     3721 ggctcatttc gatgtcttca cgtgtgatgt cgcgcagata accgggatcg tcgaaagcca
     3781 ggtactgtcc ctgttttgtg aaccagcggg aatccagcac cctgcgcgtc cagaagtcct
     3841 cccaatgcgc gtagtattgc ttcagaaacg gagcggcctc cccgcccacg cagcgcacat
     3901 accactcatc gagcaatgcg tcgacatctg cgttgggatc ccattgtaat ttaagggaca
     3961 cgtagagctt ggggccttcc ccccagttgg catacgcctc ggcatacatg gcacgcaccc
     4021 cgttggcata accgaaccgg tagtagtcgg ccatgtgatg aaaccacacg cggggaaggc
     4081 agaacggcga gccatagatg tagtcatacc agcccagcag cgggcttcgt gcgttccagt
     4141 cccgggtaag ggcctcgccg gcctcgcgca attcggggtc tttccacttc atgcggtcgt
     4201 aggtcatgta cggaatcagc cggggatgaa cggaggtgtg ctgcggcggt gcggcgactt
     4261 cgctgtaggc caggcatccg aaccatttgt ccgggaatcg ctcgagcacg ccttcgatca
     4321 cctggttggc ccagtcatag tacaggtcgg agtaatccac acagccgaga aagtttttct
     4381 cgccggaaag ccgtgccaga caccgttcgc actggcaata accgctgctg tcgtttgcgc
     4441 cgagagaata cgacgtggcg gcggggttag tctcaaaata ttcgatgatg ttcttgaccg
     4501 cttcctccac gatgcccggc gcggtaaaac agggttgcca gtagtgggcg ttgttgtccg
     4561 ggggcaggaa tcgcttgccg ttcaggattg ggaaaaactc gggatggctc gcggtgtagg
     4621 tctcgggtgg aaacaggtgg agcagataat ggtgaaaact gacccggccg tgcatgcggt
     4681 tgcgtcgcgc ccacgtggtc tgtgcctcgc ccggcaagcc gctgaacaag cgggaaaaga
     4741 tggccggcgc ctggcgcagc ggttccggcg ggaccgacag gctgcgggct gcgggcacgt
     4801 catcgccgtg ttctccgggg aggagccagc gcacccccac gtaccgttcg aggaactcgt
     4861 aaacgccgaa ctccgtgccc caatccgtgg ggcctgcgat cacgaccgtg ctggcgtccg
     4921 gaaatgcgat gtcgaaccca tcttcgtcga ggccggcctg gtcgggggtt tgccaggccg
     4981 ctccccggcc cacgcagaca acggcgcctt cgtccggcag cgcgctggtc acgggcaacc
     5041 gcgccccggt ggcccgctcc acgtactccg ccaggagctg
//
LOCUS       DAOPBQ010000244         5074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_13936_length_5074_cov_3.874278, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000244 DAOPBQ010000000
VERSION     DAOPBQ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13936_length_5074_cov_3.874278"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PKW60_08780"
     CDS             complement(<1..662)
                     /locus_tag="PKW60_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99564.1"
                     /translation="MIRRGFSPAGRTAFAAALALVCAAAAFGQPAIEELLGKMPAESS
                     QEIVAVSQSLIALGPEAIQGVCATLVPSGGDDVNARFLLSGLADYVSRGDVEEARAMV
                     SGALIQALGAAEDPYVKTFLMSMLQVCGRAEAVDALAGYLGDDVLVEPATRALLTIRT
                     ENVAPALKAAFEGAQGARAVTLMKALGELRQADVADKIVPFTRSADRETRLVAYQALV
                     EMG"
     gene            complement(735..2009)
                     /locus_tag="PKW60_08785"
     CDS             complement(735..2009)
                     /locus_tag="PKW60_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99565.1"
                     /translation="MKLKRSITRRNFLRNGALTFAGIAVLPRRVLGAPGEPSPNEELT
                     KAIVGVGGMGRGHIGMPGARLVAVCDVDSDHLDQALKMSPEGTKGYRDFREVLERPDV
                     DIVHVPTPPHWHALISIAAAEAGKDVWCEKPMTRTIGEGKKVVEAIQRNGRIFRLNTW
                     FRFQGDFYGFGTTVAPIKKLVDSGMLGWPLKVFVSGTTGFDWKFYWSGLTDLTPEPVP
                     ETLDYDFWLGPAPYKPYHPHRVHGTFRGYWDYDGGGLGDMGQHYLDPVQYFLGKDDTS
                     PVEVEVDAPQQHPDAASSWRRIVLRYADGCEITLDGEGTDTSKPYIEGPDGKLFKGFK
                     SDIPNLEKKLAQFPDPEPQMTDFAEAVRTRTTFALNESNGHRSCTLINMAKTAVQLGR
                     NLRFDPVKQEFIDDEGANRLVYQPMRAPWQLS"
     gene            2375..3889
                     /locus_tag="PKW60_08790"
     CDS             2375..3889
                     /locus_tag="PKW60_08790"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HPJ99566.1"
                     /translation="MAEIDDLGLYAQWREGKSRSAFDELVERHAPMVYSVCRRVLNAQ
                     TEAEEAAIETFQMLAYAPSGPKFHLAAWLHAAAATRAGQHLRSQRQRPAEEPPETAGG
                     PVAWDSIQGKIDSALTAVPWQARIPLIGQYIEDKTLAEIAQEIGLDRQRVITDGVTGI
                     ETLRKQLATHGVEISSSRLKELLSTQMVEPVPSGLAPRLSERAVADPLAGRRLAAGFE
                     VDETIRKRSRVSWALPLAAVALVAGLIFWFTGSGQPQDAAPPAEPAPQEIAQAAAPVE
                     SPGPRVPRSLQRPEGSAIAQSSPAQAQAAAALAYQGVVRDRSGSAVANARVFLGALPA
                     APEDVEPAAESGPMGAFGLSEVPAGTRQISIWHAAYKPATAALTPDTGSPLEVILAPA
                     AQVSGIVTYGGSPAARQSVELVDASGKTLRTQTRDDGSYRFTGAEPGLVQLRANLDAM
                     EPANRRRTMYSTAVLETGLTTDVDFTFGVLDAAVEGVAMFNDEPARGAKIVVCI"
     gene            3959..4423
                     /locus_tag="PKW60_08795"
     CDS             3959..4423
                     /locus_tag="PKW60_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99567.1"
                     /translation="MPSGPAVLQLQGLRTGRTEHLRSLSAQIRSGQTLPCDFMLYGTS
                     SVIAQVSGVPPGATGIVAVIEPGTSLLGLNWPEYHLTPVHLLARIPLGQDGEYTFEGL
                     EAGACTICAYATPPAGPAVEPGETTTAPPPLYDAAGLTLQPNGETLIVLQLR"
     gene            complement(4427..>5074)
                     /locus_tag="PKW60_08800"
     CDS             complement(4427..>5074)
                     /locus_tag="PKW60_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPJ99568.1"
                     /translation="ALEKVAAAHRDRGFDVTALQYDQGDEASVLALRDQILEQAGGID
                     ILVNNSVARPMKNGYQSDASTFAHSMAVNATGLFVITRAFGDVMAENGRGSIINVGSI
                     QGLIAPDPSIYRGTTMSGWAPDYFFHKGGMVNFTRFIGSYYGLKNVRCNCIAPGGYQT
                     PDHPEPFVRQYSDKTFLGRLASDTDLKGVIVFLASDASAYITGATIPVDAGYTAK"
BASE COUNT          925 a         1633 c         1616 g          900 t
ORIGIN      
        1 cccatttcaa ccagcgcctg gtacgcgacc aaacgggtct cccggtccgc tgagcgggtg
       61 aatgggacga tcttgtccgc cacgtccgcc tggcgcaact cgccgagggc cttcatgagg
      121 gtcacggcac gagccccctg ggcgccttcg aacgcggctt tcaatgccgg cgcaacattc
      181 tctgtgcgaa tggtaagcag ggcccgcgtc gcaggttcca cgagcacatc atcgcccagg
      241 tagccggcga gcgcgtcgac ggcttcggca cggccgcaga cctggagcat gctcatcaga
      301 aacgtcttga cgtacgggtc ctcagcagcc ccaagcgcct gaataagcgc gccggatacc
      361 atcgcgcgcg cttcttcgac gtcgccgcga gacacatagt ctgccaggcc gctcaaaaga
      421 aaacgcgcgt tgacgtcgtc accgcccgac ggcaccaggg ttgcacagac accctgaatg
      481 gcctctggcc cgagcgcgat caggctctgg ctcacggcga cgatctcctg ggacgattcc
      541 gcgggcattt tgcccagcag ttcctcaatc gcgggttgcc cgaaggcggc cgcagcgcag
      601 accagagcca gtgcggccgc gaacgccgtt ctgcccgccg gcgagaatcc tcgacgtatc
      661 atgttcattt cctttcctcg tagtatcgtc gcgggcgcat tcgcgcccgg tagcgtccat
      721 ttaacacggt gcgatcacga cagttgccag ggggcgcgca tgggctgata cacgagccgg
      781 ttcgcgcctt cgtcatcgat aaactcctgc tttaccggat cgaagcggag gttccggccc
      841 agctgcacgg cggttttggc catattgatt agcgtacagg agcggtggcc gttggattca
      901 ttgagcgcaa acgtcgtgcg ggtgcggacc gcctccgcga agtcggtcat ctgcggctcg
      961 gggtcaggga actgggccag tttcttctcg aggttcggga tgtccgactt aaaccctttg
     1021 aacagtttgc cgtcgggtcc ctcgatgtac ggtttggatg tgtcggtgcc ctctccgtcc
     1081 agggtgatct cgcatccatc cgcatagcga agaacgatcc gccgccagga gctggccgcg
     1141 tcgggatgct gctggggcgc atcgacttcc acctcgaccg ggctggtgtc atccttgccg
     1201 aggaaatatt gcacgggatc gagataatgc tgacccatgt cgcccaggcc gccaccgtcg
     1261 taatcccagt atccgcggaa ggtgccgtgc acgcggtgcg gatggtaggg cttgtacggc
     1321 gcgggaccaa gccagaagtc gtaatcgagg gtctccggaa cgggttccgg cgtgagatcc
     1381 gtcagaccgc tccagtagaa cttccagtcg aaccccgtgg ttccactgac aaacactttt
     1441 aacggccagc caagcatgcc gctgtcgacg agcttcttga tcggggccac ggtggtgcca
     1501 aatccataaa aatcgccctg gaaacggaac caggtgttga ggcggaagat gcggccattg
     1561 cgctgaatgg cttcgactac ctttttgcct tcgccgatgg tccgggtcat gggtttctcg
     1621 caccatacgt ccttgccggc ctcggcggcg gcaatcgaga tgagtgcgtg ccaatggggc
     1681 ggcgtgggca cgtgcacaat atcgacatcg ggccgctcga gcacttcccg gaaatcgcga
     1741 tagcctttcg tgccttcggg cgacatcttg agcgcctggt cgagatggtc ggaatccaca
     1801 tcgcacacgg caaccagccg cgcgcccggc atgccaatat ggccgcggcc catgccgccc
     1861 acgccgacga tcgccttggt gagttcttcg ttgggcgaag gttctccggg ggcgccgagg
     1921 acgcggcgcg gcagcaccgc gatgcccgcg aatgtcaggg cgccgttccg aaggaaattc
     1981 cgccgtgtaa ttgaccgctt aagcttcatg tctgtctcct tacattggcc gcgacttgcc
     2041 gtgccggaca cactgggctg ccctcgcgca ttcgactgcg atgtggcgcc attcgcgaaa
     2101 cagcctcccc cgcccccggc cgcaccggca agaaataaac ggagcctggg ctgaatagag
     2161 ctagagtatc gctcgaatac gtccgcactt caagtaaatg aggcatgaaa acgttgatac
     2221 aaccgtaggg cgcctgcagc ccgtttcggc ggcaagcggc agagtccggt cccgggaaaa
     2281 tagccgggca gcgcgcgcgt cgtgtccgta tatggccact gaggctcata atgggtataa
     2341 tcgccctgcg aaacaaggta tccgggggtg cgcaatggcg gaaatcgacg acctgggact
     2401 ctacgcgcag tggcgcgaag gtaagagccg gagcgcgttc gatgaattgg tggaacgcca
     2461 tgcaccgatg gtgtacagcg tctgccgcag ggttctgaat gcgcagacgg aagcagagga
     2521 ggcggccatt gaaaccttcc agatgctcgc ctacgccccc tccggcccta agtttcatct
     2581 agcggcctgg ctgcatgccg ctgctgccac ccgcgccggg caacacctgc gcagccagcg
     2641 ccagcgcccc gcggaggaac caccggaaac cgctggcggc ccggttgcct gggactctat
     2701 tcaaggcaag atcgactccg cactgaccgc cgtgccgtgg caggcgcgta tcccactcat
     2761 tggccagtac atcgaagata agacccttgc cgagatcgct caggaaatcg ggctggaccg
     2821 ccagcgcgtg atcacggacg gcgtcacggg aattgagacg ctgcgcaaac aactggccac
     2881 gcacggcgta gaaatcagca gctcgcgcct taaagagctg ctttccacgc agatggtcga
     2941 acccgtgccc tccggattgg cgccccgcct gtctgaacgc gcggttgcgg acccgctcgc
     3001 cgggcgccgc cttgccgcag gtttcgaagt ggatgagacg atccgaaagc gctcccgcgt
     3061 atcctgggcg ctgccgcttg ccgccgtggc cctggtggcg gggctgatct tctggttcac
     3121 cggcagcggg cagccgcaag acgccgcgcc tccggctgag cctgcgccgc aggagatcgc
     3181 tcaggcagca gccccggtgg aaagccccgg accgcgcgtg ccgcggagtc ttcagagacc
     3241 ggaaggcagc gccatagccc aatcttcccc cgcgcaggcc caggctgcgg cggcgctggc
     3301 gtatcagggc gttgtccgtg accgtagcgg cagtgccgtc gccaatgccc gggtttttct
     3361 tggcgccctg cccgcggcgc cggaagacgt cgaacccgcc gcggaaagcg gcccgatggg
     3421 cgcgttcggc ctgagtgagg tgccggcagg cacgcgacag atcagcatct ggcacgcggc
     3481 atacaaaccc gcaacggccg ccctgactcc ggacacgggc tccccgctcg aggttattct
     3541 ggcgcccgcg gcacaggtga gcggcatcgt cacgtacggc ggctctccgg ctgcccggca
     3601 gtccgtggaa ctggtcgatg ccagcggcaa aaccctgcgc acccagaccc gggacgacgg
     3661 gagctatcgg tttactggcg cagaacccgg cctggtgcag ctgcgcgcaa atctcgacgc
     3721 catggaaccg gcaaaccgcc gccgtaccat gtacagcacg gccgtgctcg aaaccggact
     3781 gaccaccgac gtggacttta cctttggcgt cctcgatgcg gccgttgaag gcgtcgcgat
     3841 gttcaatgac gaaccggcgc gcggggcaaa aattgtggtt tgtatttaaa ccctcagcgg
     3901 aggcgaggag tagattgagg cgtctgttta ggcggatgga acttatctga tcgaggccgt
     3961 gccctccggc ccggcggtgt tgcagctgca gggcctgcgc acgggccgca ccgagcatct
     4021 gcggagtctc agcgcgcaga tccggagcgg ccagacgctt ccgtgtgatt ttatgctcta
     4081 cggcacctcg tccgtcatcg cgcaggtatc cggggtgcca ccgggcgcaa cgggtatcgt
     4141 cgcggtcatc gagccgggga ccagcctcct gggtctgaac tggccggaat accatctcac
     4201 gccggtgcat ctgctggcgc gcatcccgct gggccaggac ggtgaatata cctttgaagg
     4261 gctggaggcg ggggcctgta ccatctgcgc atacgccacc ccgcccgctg gaccggctgt
     4321 ggaaccgggg gagacaacga ccgccccgcc gcccttgtat gacgcggcag gactgaccct
     4381 gcagcccaac ggcgagacgt tgatcgtcct ccagctgcgc taaaacctat tttgcggtgt
     4441 accccgcatc gacagggatg gtcgcgcccg tgatgtaggc cgacgcatcg ctcgccagaa
     4501 atacaatgac ccctttcagg tcggtatcgg acgccaggcg gcccaggaac gtcttgtcgc
     4561 tgtattgccg cacaaacggc tcgggatgat ccggcgtctg gtagcccccg ggggcgatgc
     4621 agttgcaccg gacgttcttc aggccatagt agctcccgat gaaacgcgtg aaattgacca
     4681 taccgccctt gtggaagaaa tagtcgggcg cccagccgct catggtggtt ccccggtaga
     4741 tcgacggatc gggcgcgata agcccctgga tggaacccac gttgatgatc gaaccgcggc
     4801 cgttttcggc catgacgtcg ccgaaggcgc gggtgatcac aaacaagccg gtcgcgttga
     4861 ccgccatgct gtgcgcaaag gttgaggcat cgctctggta gccgttcttc atcggccggg
     4921 ccacggaatt gttcacgagg atatcgatgc cgcccgcctg ctcgaggatc tggtcgcgca
     4981 gggcaagcac cgacgcttca tcgccctggt cgtactgcag cgcggtcaca tcgaaccccc
     5041 ggtcgcgatg ggcggccgcc accttctcga gggc
//
LOCUS       DAOPBQ010000245         5026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14094_length_5026_cov_3.795816, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000245 DAOPBQ010000000
VERSION     DAOPBQ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14094_length_5026_cov_3.795816"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="PKW60_08805"
     CDS             complement(<1..1462)
                     /locus_tag="PKW60_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPJ99569.1"
                     /translation="MAARWMAALVMGAFAFVPAFAGEGSLKVYASGEVVEVGQVFTIL
                     VEVSGNRIGDVRMPQIRQLHIEKRASFQRSEYAAVLGQEVKLTQKLGFNASALQEGDV
                     TFPSLTVEIDGAPVTSKPFVIRVVRAGSERPSPPPAATGPDEDKATPLTLEDVAFLNT
                     TADKTEVYVGEPVSLTMAFYRLDDNAIRVSAPEGGIELTFPETAGFYSIPERPEEVDG
                     AYVERGGRRYRGARWRQTLFATNSGDLVIPPWEWSAGVYAHTIRGPVSVRLDLHTDPI
                     PIKVKPLPKRPRNFNGAVGQFRFQAQVTPRDTTQNMPVTLIIRVNGTGNPAAIAELQL
                     PALEGAHVSEPERAIQPIMDPDGVTAETTFSYQITPFNAGPLAIPPVEFCYFDPDKGQ
                     YVTEKSSAFIVNVEASGEVARRHVAGATIAPEPERPDLSQAGLLPIVTAVNHLRRGPA
                     SRTGEAAVVLFPPLAYGLFAFWTARQRRFARDHTFAR"
     gene            complement(1462..2409)
                     /locus_tag="PKW60_08810"
     CDS             complement(1462..2409)
                     /locus_tag="PKW60_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99570.1"
                     /translation="MDRRKEYRNEIHGLAAAVVCLCGVLSAVAAPADSFREQLKYGNA
                     LLNSGDFEGAREVYRGLQLEHPESEIVYYSLGCADYEEGAGAVALQDPEGAIEFFSSA
                     RDAFQRAKTSRDKSLRIDASYNHANAMAQIAKQTASLGDNKATITAFEETVAAYEGVL
                     ERYPDHKQARHNLDHVRYLLKRMLQVPPPDEEQQEGEQGGQQEQQQDSQEQQNGDQQQ
                     DQQEQQDDQGEKDGQEDSDQQGSQQPEQEEGAPQQESKQQTGASATLEEEPGEGDREE
                     RQDRQTIEAILQSLESQDKREQQDLRTSQPDSHVRKEWW"
     gene            complement(2402..3502)
                     /locus_tag="PKW60_08815"
     CDS             complement(2402..3502)
                     /locus_tag="PKW60_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPJ99571.1"
                     /translation="MKFGFGFPLAQLPLWLTVAFLALLLSAAGLYWLEIRRKRRIARF
                     VQHALLPRLVGNYDERVRRPLYWFTVLGFAFFLLALAQPHWGRAWKEVRKHSRDVMIC
                     LDISESMRATDLPPNRLERAKQKIVSLVDHSPGDRFGLVAFTGAAGLMCPMTLDHGYF
                     KAVLSAVDTDSISREGTDIAAALASAVEVFKDEDTKRDEFARDTRAILLITDGEEVSG
                     NAIEMAKEASAYCRIYVIGVGDPEGAEIEIPGWMAREGVRGAAARELSHLSKLDEETL
                     IRVADAGNGPYVAATPDDWDIEQIRGAMARLESRLVESDVRLQLVNRYQWPLTVAVLL
                     FAAEGFWLAAMPWLRSWRTWRVSQPRRETQHG"
     gene            complement(3512..4555)
                     /locus_tag="PKW60_08820"
     CDS             complement(3512..4555)
                     /locus_tag="PKW60_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPJ99572.1"
                     /translation="MNWLQINTFMHPRLLLLAPLVILLLALESFARAPGTISISTGET
                     LRRVGSAKRAVLRRLPAFLRALALLLLLVALARPLKGLQPRLDRADVVDIMLCVDVSG
                     SMAAQDFESRGARKNRLEVTKDAVRDFIESRKQKPGDRYGLDRLGLILYAGYAWTQCP
                     LTLDYGVLDRELELASINERDPRKQGTAIGSAIGLAASRLRKSEARTKVIILLTDGLN
                     NSGELDPITAARLAQEYGIRIYTIGAGSQGEVLVPRRSLWGNVMMPAQMPIDEESLQK
                     IAEITGGRFYRATDTKSLIGAYEEINELEATEIEIGDYYEYEEGFFPWALFGGALMCA
                     AVISRRLWFEPIP"
     gene            complement(4552..>5026)
                     /locus_tag="PKW60_08825"
     CDS             complement(4552..>5026)
                     /locus_tag="PKW60_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99573.1"
                     /translation="GRLAGAATGIALLSALLLFVLKRLKRARVEIPRPPWEAAFERLH
                     ALDARKLPETGAYQEFYVELSDTLRVYIEGRFRIHAPEQTTPEFLSEAARSGALSEAH
                     QRRLGRFLKHCDRVKFAQYEPTIEEMERSFALVLKFIDETIPVEEPGPAPKEEAA"
BASE COUNT          950 a         1624 c         1449 g         1003 t
ORIGIN      
        1 agcgtgcgaa cgtatgatcc cgcgcaaacc ggcgctggcg ggcagtccag aacgcaaata
       61 ggccataggc caggggcgga aacagcacaa cagccgcctc gcctgtccgc gaggccgggc
      121 cgcgccgcaa atggtttacc gctgtcacaa tcggcaaaag gccagcctgt gacaaatcgg
      181 ggcgctcggg ctccggggca atcgtggcgc cggccacatg gcgccgcgca acctcgccgc
      241 tggcctcaac attgacaatg aacgcggatg atttctcagt gacatattgc cctttatccg
      301 gatcgaaata gcagaattcg acggggggaa tcgccagagg gcccgcattg aacggggtaa
      361 tctgatacga aaaggtggtt tccgccgtga cgccgtccgg gtccatgatt ggctggatgg
      421 cgcgttcggg ttcggacacg tgtgcgccct ccagggcggg caattgcagc tcggcaatgg
      481 cggccgggtt gccggtgccg ttcacccgga taatcagcgt gaccggcata ttctgcgttg
      541 tgtcgcgtgg tgttacctgc gcctggaagc ggaattgccc caccgcgccg ttgaagttcc
      601 gcggccgctt gggcagcggc ttcactttga tggggatcgg gtccgtatgg agatccagac
      661 gcacagatac cggcccccgg atggtatggg catagacccc ggcactccat tcccaggggg
      721 gaatgaccaa gtcgccggaa ttcgtggcaa aaagggtctg acgccaccgc gctccgcgat
      781 aacgcctccc cccacgctcg acataggcgc cgtcaacctc ttccgggcgc tcggggatac
      841 tgtaaaaccc cgcggtctcc ggaaatgtga gttcgattcc tccctccggc gcactcacgc
      901 ggatggcgtt gtcgtccagg cggtaaaacg ccatcgtaag cgaaacgggc tctcccacat
      961 acacctcagt cttgtcggcc gtggtattca aaaacgcgac atcttcaagg gtcaaaggtg
     1021 tggctttatc ctcatctggc ccggttgcgg caggcggagg cgaaggccgt tcgctacctg
     1081 cacgtactac ccggatcacg aatggcttgc tggtgacagg cgccccgtcg atttcaaccg
     1141 tgagagacgg gaaagtcacg tcgccttcct gcagtgcgct ggcattgaag cccagcttct
     1201 gagtgagctt cacctcctgt cccaaaactg cggcatattc cgagcgctgg aaggaggcgc
     1261 gcttttcgat atgcaattgg cgaatctgcg gcatgcgcac gtcaccgatt ctgtttcccg
     1321 acacttcgac caggatggtg aacacctggc ccacttccac gacctcgcca ctggcataca
     1381 ccttgagaga gccttctccg gcaaacgccg ggacgaaagc gaatgcgccc atgaccagtg
     1441 ctgccatcca ccgcgcggcc atcaccacca ttccttgcgg acgtgcgaat caggctggct
     1501 ggttcgcaaa tcctgttgct cgcgtttgtc ctgcgattca agcgactgca ggatcgcttc
     1561 gatggtctgc cgatcctgcc tttcctctcg atccccttcg ccaggctctt cttcgagcgt
     1621 cgccgaggct ccagtctgtt gctttgattc ctgttgtggc gccccttcct cctgctccgg
     1681 ctgctgcgag ccctgctgat cagaatcctc ctgcccgtcc ttctcgccct gatcatcctg
     1741 ctgctcttgc tgatcttgct gctggtctcc gttttgttgt tcctgcgagt cctgctgctg
     1801 ttcctgttgt ccgccttgct ctccctcctg ctgctcctcg tccggcggag ggacctgcaa
     1861 catccgtttc agcagataac gcacatgatc caggttgtgg cgtgcctgtt tatggtcggg
     1921 gtagcgttca agcacccctt cgtatgccgc gaccgtctct tcgaatgcgg taatggtggc
     1981 cttgttatcg cccagagatg ccgtctgctt ggcgatttgc gccatcgcat tggcgtgatt
     2041 gtacgaggcg tcgatacgca gtgatttgtc gcggctcgtc ttggctcgct gaaacgcatc
     2101 gcgggcggaa gaaaagaact cgatagcgcc ttccggatcc tgcaacgcca ccgcgccggc
     2161 gccttcttcg taatccgcac atcccaggct gtagtagacg atctcgctct ccggatgttc
     2221 gagctgcaga ccccggtaga cttcccgggc cccttcgaaa tctccgctgt tcagcagcgc
     2281 attgccgtat ttgagctgtt cgcggaacga atccgccggc gccgcaacag cagacagcac
     2341 gccgcacaga catacgaccg cggccgccag gccatgaatc tcgtttctgt attcttttcg
     2401 cctatccatg ctgtgtctcg cgccgcggct gcgaaacgcg ccacgtcctc caggagcgca
     2461 gccacggcat cgcagccagc cagaatcctt ctgcggcaaa caacagcacc gcaacggtca
     2521 ggggccactg gtagcggttt acgagttgga gccgcacatc actctcgacg agccggcttt
     2581 cgaggcgcgc catcgccccg cgtatctgct cgatatccca atcatccggc gtagccgcaa
     2641 catacgggcc gtttccggca tccgcaaccc ggatcaaggt ctcttcgtcc agtttggaaa
     2701 gatgactcag ctcacgagca gccgccccac ggaccccttc tcgcgccatc catcccggga
     2761 tctcgatctc cgcgccctcg ggatctccca caccaatcac gtagatgcgg caatatgctg
     2821 acgcttcttt ggccatttcg atggcgttcc cggacacttc ttcgccgtcg gtgatgagca
     2881 ggatcgcacg ggtatcccgc gcgaactcgt cgcgtttggt gtcctcatct ttgaagactt
     2941 ccacggcgct ggccagcgcg gccgcgatat ccgtgccttc ccggctgatc gagtccgtat
     3001 cgacggcgct gagcaccgcc ttgaaatacc cgtggtcgag cgtcatcggg cacatgagcc
     3061 ctgccgcgcc ggtgaacgca accaggccga accggtcgcc aggtgaatga tcgaccagcg
     3121 agacgatctt ctgcttcgcg cgttccagcc ggtttggcgg caaatccgtg gcgcgcatac
     3181 tctccgagat gtcgaggcag atcatgacat cgcggctgtg tttgcggacc tccttccagg
     3241 cccggcccca gtgaggttgt gcaagcgcca gcagaaaaaa cgcgaacccc agcaccgtaa
     3301 accaatacag cggccgccgc acgcgttcgt cgtagttgcc cacgagccgc ggaagcaatg
     3361 catgttgcac gaagcgggcg atccggcgtt tacggcggat ttcgagccag tacagtcctg
     3421 cggccgacag cagcagcgcg agaaacgcca ccgtcagcca caagggcaac tgagccaatg
     3481 gaaatccaaa cccgaacttc acgaaaacct cctaggggat gggctcgaac cacaggcggc
     3541 gcgaaatgac tgccgcacac atcagcgcgc cgccgaagag agcccacggg aaaaaccctt
     3601 cttcatactc gtagtagtct ccaatttcga tctcggtggc ctccagttca ttaatttcct
     3661 catacgcccc gatcagcgat ttcgtatcgg tagcgcggta gaaacgcccg ccggtgatct
     3721 cggcgatttt ctggagggat tcctcatcga tgggcatttg cgcgggcatc atcacattgc
     3781 cccacaggct gcggcggggc acgagcactt caccttgcga acctgcgcca atggtataga
     3841 tccggatccc gtactcctgt gccagccgcg cggccgtgat gggatcgagt tcgcccgaat
     3901 tgttcagccc gtccgtcaac agaataatga ccttggtcct ggcctccgat tttcgcaaac
     3961 ggctggccgc cagcccgatg gctgagccga tggcggtgcc ctgtttccgg ggatcacgtt
     4021 cattgatgct ggcgagctcc aattcgcgat caagcacgcc ataatcgagc gtcagagggc
     4081 actgggtcca ggcataccct gcgtagagga tcaatccgag ccgatcaagg ccgtagcggt
     4141 ccccgggctt ctgtttccgg ctttcgatga aatcgcgcac ggcgtccttg gtcacttcga
     4201 ggcggttttt ccgtgccccg cgcgactcga aatcctgggc cgccatgctt ccggatacgt
     4261 cgacacacag cataatatca accacgtcgg cccgatcgag ccggggctgg agccctttca
     4321 gaggccgggc cagcgcaacc agcagcaaca gcagcgccag agcgcgcaga aaggccggca
     4381 accgcctcag aaccgcacgt ttggcgcttc ctactctccg gagggtctca ccggtagaaa
     4441 tgctgatcgt gcccggcgcc cgtgcgaaac tctcgagcgc aagcaagagg ataaccagag
     4501 gcgccagcag caggagccgg ggatgcatga aggtattgat ttgaagccag ttcatgccgc
     4561 ctcttccttt ggcgcgggac ccggctcttc aaccgggatg gtttcgtcga taaatttcag
     4621 caccagggca aagctgcgct ccatttcctc gatggtgggc tcgtattgcg caaacttcac
     4681 tcggtcgcag tgcttgagga aacgcccgag gcgccgctgg tgggcctcgg aaagcgcccc
     4741 actgcgcgcg gcctccgaca ggaattcggg cgtggtctgt tcgggcgcat gaatgcggaa
     4801 ccggccctcg atgtacacac gcagcgtgtc cgacaactcc acataaaact cctgatatgc
     4861 tcccgtttcg ggaagtttgc gggcgtcgag cgcatgcagc cgctcgaacg cggcctccca
     4921 cggcgggcgc ggaatttcaa cacgcgcgcg cttcaagcgt ttcaacacga aaagaaggag
     4981 cgccgaaagc agcgcgatgc ctgttgcggc gccagccagc cgcccg
//
LOCUS       DAOPBQ010000246         5022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14109_length_5022_cov_3.376082, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000246 DAOPBQ010000000
VERSION     DAOPBQ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14109_length_5022_cov_3.376082"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PKW60_08830"
     CDS             <1..918
                     /locus_tag="PKW60_08830"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPJ99574.1"
                     /translation="GVVLTIGSFDGVHLGHRAIVREVVEKARQRDGTPCVLTMRPHPR
                     EFFSPERPPNLLTDERKKARLLAEIGIQVLFVLPFNRQVAGMSRETFVEEIVVNRCHA
                     RDVVVGHDFRFGKGAEGNFEFLQSFCTGRGIGVEQVAPIQVDGERVSSTLVRECLLQG
                     DIDRAAQLLGRRYSIAGQVVRGRGIGAQLGFPTANIKPHHTAVPAQGVYAARATIQGV
                     SYPAAVNVGIAPTISHTDLTIEAHILDFSRDIAGGGIELEFFQRLRPERKFPSRDALV
                     EQIARDVQSVRMYFAADDTSAGPRGPETS"
     gene            1026..2897
                     /locus_tag="PKW60_08835"
     CDS             1026..2897
                     /locus_tag="PKW60_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPJ99575.1"
                     /translation="MNSRKRVAFLCAVLALLLVVSCSTSRYKEKADAEVYPIISEKSL
                     EVPGSPQDFTIDQEETLSLEGLPTLESEDETLGDSAPHEAGFYILSLEKALSVAVKRS
                     RDYQSQKESLYESALALTLQRHRFDPIFAGRFRGDYNRTTRDVLRPTDAALVARELPR
                     ILERTGELTGGTGALLNSYADVVRAAAATTGVAGETRAHIVDERSVTGSSNLGFDLLL
                     AGGARIAVDLTSNFLRFVTGDPRVATSSTLQATVTQPLLSGAGRDVVLENLTQAERNV
                     LYQLRSFTRYRKNFAIDIANAYYRVLQNRDAVRNNWSGYLAFQDNAKRQRALAQAGRI
                     KVAELGRNEQAELQARDRYIASVQNYADSLDQFKIKLGLSTDAPILLDQGELELLREK
                     GLDHPNLAPEQAIEIALVSRLDLMVARDRVEDTERKLLVAANALQPNATLVAQGRASS
                     YGQDRFQSIDFDRAEASIGLSVDPLFDRKAERNAYRSALIDYARSVRSLEEATDLIKL
                     DVRQAWRNLQQAQVSYQIQEIGVQLNERRVREQELLLQAGRGSAIDQVDAQNSLIASQ
                     NELTAALVQHTIARLSFWRDMGILFIKEDGQWEDITDDNYSGEAATESVEEEPSPVS"
     gene            2833..4710
                     /locus_tag="PKW60_08840"
     CDS             2833..4710
                     /locus_tag="PKW60_08840"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPJ99576.1"
                     /translation="MTIIPERLPPNPWRKSLPLFRKWWKYIAAAVFLLALVTLAARDR
                     ERSAGVISYRVQRGPLRITILEKGEVEALESQEIKSEVWGETKIISIVDEGYLVTEED
                     VQNKMVLVELDSSELKDRLTQKEIEFQGTRASLTEAQEQYQIQLKQNESDIKAAELQV
                     KFKRMDLEKYLGSKLALDLLDRLGIKEISAEDIEEEDRAARALEFPSPQAADSSDAEV
                     ASPSEVETEEMEALQVEVVLTTEAQKIRDALDFLELAKDARLAGEAVQKRQKFETDIL
                     LAEEDLSTARDTYEWTAKLAQEDFVTETQRRQDEMAVTRSEIALESAKLAQELFITYE
                     FPKQAETLLSDYEEALRGLSRTVKKARSQIAQSDAQLKSAEARYSLEKLQRDELVEQI
                     GKCTIVAERAGLVVYGDNESRRYGTEPIRAGATVYRNESIITIPDMTKMALKVNIHES
                     HIQKVQKGQKASIAIEAFADRELTGEVSKIGMLPDEESSWLNPDLKVYKTTVVIDGSH
                     DWLKPGMTAKVDILIEELHDVLYVPIQAVFTFEGKRVTYLAQLGREPERRYVETGQFN
                     GAFVEVKSGLNEGDVVLLIQPETPSRTGEKPAENIPGELPSEAPAETPAPENVQASAA
                     E"
     gene            4879..>5022
                     /locus_tag="PKW60_08845"
     CDS             4879..>5022
                     /locus_tag="PKW60_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ99577.1"
                     /translation="MKLTIKLGLKSLWLHRLRSLLTVLGIVFGVCSVIAMLAIGEGAS
                     YEAQ"
BASE COUNT         1146 a         1476 c         1480 g          920 t
ORIGIN      
        1 ggcgtcgtcc tgaccatcgg gagtttcgac ggcgtgcacc tggggcaccg cgccattgtg
       61 cgcgaagtcg tggaaaaggc ccggcaacgc gacggcaccc cgtgtgtttt gaccatgcga
      121 ccccatcctc gtgaattttt ttctccggag cggcctccga acctgctcac ggatgagcgc
      181 aaaaaggcgc gtctcctggc ggagatcggg attcaagttc tcttcgtgct cccattcaac
      241 cgccaagtgg ccggaatgtc ccgggagacg ttcgttgagg agattgtcgt taaccgctgc
      301 catgcgcgag acgtagtcgt cgggcacgat tttcggtttg gaaaaggggc cgaaggcaat
      361 tttgagttct tgcagagttt ctgcaccggg cggggcatcg gtgtcgaaca ggttgcgcca
      421 attcaagtcg acggagaacg ggtgagcagc accctggtca gagaatgcct gcttcaaggc
      481 gatatcgacc gggccgcgca gctgctcggc cggcgctatt cgattgcggg acaggtggtc
      541 cgtggccggg ggatcggggc tcaactggga tttccaacgg ccaacatcaa gccgcatcac
      601 accgccgttc ccgcacaggg cgtctacgct gcacgggcga cgatacaagg tgtatcctat
      661 cctgccgcgg tgaatgttgg aattgcgccg acaatttccc atactgatct gacaatcgag
      721 gcacatatcc tggatttttc ccgggatatc gccgggggag gtatcgagct cgaattcttc
      781 cagcggttgc gtccagaacg caagttcccg tcacgcgacg cgctcgttga acagatcgcg
      841 cgcgacgtgc agagcgtacg aatgtatttt gccgcggacg acaccagtgc tggacccagg
      901 ggacccgaaa catcatgact tccgcaattg cccggtaccg ggtcggaagc gtagactata
      961 gtagactgaa gtaaacgtga tgtctgaggg cgcagcagcg cctgagatag cggggtaatt
     1021 gtgtaatgaa cagccgaaaa cgggtagctt ttctctgtgc agtgcttgct ctcttgcttg
     1081 tagtgagttg ttccacatcg cgctacaaag agaaagctga tgcggaagtc taccccatca
     1141 tctcagagaa atcactcgaa gtccccggca gcccccagga ttttacgatc gaccaggagg
     1201 aaacactatc gctcgaaggg ctgcccaccc tggagagcga agatgagacc ctgggtgatt
     1261 ccgctcctca cgaggccggc ttctacatcc tgtcgctcga gaaagcactg agcgtcgcgg
     1321 tgaagcgcag cagggattat caaagccaga aagagtccct gtatgaaagc gccctggcat
     1381 taaccctcca gcgtcatcgt ttcgatccca ttttcgccgg ccgtttccgc ggagattaca
     1441 accggaccac ccgggatgtc ctccgcccga ccgacgcggc ccttgtcgct cgagaattgc
     1501 ccaggatcct cgagcgtacg ggcgaattaa ccggcggcac cggggccctg ctcaacagct
     1561 acgccgatgt ggtcagggcg gccgccgcta ctaccggggt cgccggcgaa acccgtgccc
     1621 atatcgtcga cgagcgatcg gtcaccggaa gcagcaatct cggatttgac cttctgctcg
     1681 ccggaggcgc ccgcattgcg gttgacctta cctccaattt cctgcgcttt gtgactggcg
     1741 acccgcgcgt ggccacctcc tcgaccctgc aggcgaccgt cactcagccg ctgctgtcgg
     1801 gagccgggcg tgacgtcgtt cttgaaaacc tgacccaggc ggagcggaac gtcctgtatc
     1861 agctgcgctc gttcacccga taccgcaaga atttcgcaat cgacattgcc aatgcgtact
     1921 accgcgtgct gcagaaccgc gatgcagtac gcaacaactg gagcggatat ctggcgtttc
     1981 aggacaatgc caagcggcag cgtgcgctgg cccaggccgg acgcattaaa gtcgctgaac
     2041 tgggccgaaa tgaacaggcc gaactgcagg cccgcgaccg ctacatcgcg tccgtgcaaa
     2101 attacgccga cagcctcgac caattcaaaa tcaagctggg cctatcgacg gacgcgccaa
     2161 ttctgctgga tcagggggag ctggagcttc tgcgtgaaaa aggcctggat caccccaacc
     2221 tcgcccccga acaagccatt gaaattgccc tcgtctcacg acttgatctg atggtcgccc
     2281 gcgatcgcgt cgaagatacc gaacgcaagc tcctggttgc cgcaaatgcc ctgcagccca
     2341 acgcgaccct tgtggcgcaa ggccgggcga gcagctacgg ccaggaccgg ttccagagca
     2401 ttgatttcga ccgcgccgaa gccagcatcg gcctcagcgt cgatccgctt tttgatcgaa
     2461 aagccgagcg caatgcctac cgaagcgcac tcatcgatta tgcccggtcg gtgcgatccc
     2521 tggaagaagc aaccgatttg attaagctcg atgtgcggca ggcctggcgt aaccttcagc
     2581 aggcgcaggt cagttatcaa atccaggaaa tcggcgttca gttgaatgaa cgccgcgtca
     2641 gagagcagga gctgctcctt caggccgggc gcgggagcgc tatcgaccag gtcgacgcac
     2701 agaactcgct catcgcgtct cagaatgaac tgaccgcggc gctggtccag cataccatcg
     2761 cccgattgag tttctggcgg gatatgggaa tcctcttcat aaaagaagac ggacagtggg
     2821 aggacatcac agatgacaat tattccggag aggctgccac cgaatccgtg gaggaagagc
     2881 cttcccctgt ttcgtaagtg gtggaagtac atcgctgccg ccgtattctt gctggcgctt
     2941 gtgacccttg cggcccgcga ccgggaaaga agcgccggcg tgatcagcta ccgggttcag
     3001 cgggggcccc tgcgcatcac catcctcgag aagggcgaag tggaagccct cgagtcgcag
     3061 gagatcaaat cggaggtgtg gggcgaaaca aagatcatca gcatcgtgga tgagggctac
     3121 cttgtcaccg aggaagatgt tcagaacaaa atggtcctcg tggaactcga ttcctccgaa
     3181 ttgaaagatc gcctcactca aaaagagatc gaatttcagg gcacccgcgc cagtctcacc
     3241 gaagcccagg aacaatatca gatccagctg aagcaaaatg aaagcgacat caaagcggcc
     3301 gaactccagg tcaagttcaa gcgcatggac ctcgagaaat acctcggctc gaaattggcc
     3361 ctcgacctcc tcgaccggct gggaatcaag gaaatttccg cagaagacat tgaagaagaa
     3421 gaccgggccg ccagggcctt agagttccca tcgccgcagg ctgccgattc cagcgatgcc
     3481 gaagtggcca gcccttccga ggtcgaaaca gaggagatgg aggcgctgca ggtcgaagtc
     3541 gtgctgacca cggaggcgca aaagatccgg gacgccctcg attttctgga gctggcaaaa
     3601 gacgcgcgcc tggcgggtga agccgtccag aaacgccaaa aattcgaaac cgacatcctg
     3661 ctcgccgagg aagacctctc aacggcacgg gacacgtacg aatggactgc caaactggct
     3721 caggaagatt ttgttaccga aacccagcgc cgccaggatg aaatggccgt cacccgaagc
     3781 gagatcgccc tcgaatcggc gaaactggcg caggaactct tcatcaccta cgaatttccg
     3841 aaacaggccg aaaccctgct ctctgactac gaagaggcac tccggggact gtctcgaacg
     3901 gtcaaaaagg cccgctcgca gattgcccag tccgatgctc agctcaagtc ggccgaagcc
     3961 cggtattcgc ttgagaaact gcagcgcgat gaactcgtgg agcagattgg gaagtgcacg
     4021 atcgtagccg agcgtgccgg gctggtcgtc tatggcgaca acgaaagccg gcgttacggc
     4081 acggagccga tccgtgcggg tgcaaccgtt taccggaacg agtccatcat caccattccc
     4141 gatatgacca aaatggctct caaggtcaac atccatgagt cccatatcca gaaggtccaa
     4201 aaaggccaga aggcctccat cgccattgaa gccttcgccg acagggaact caccggcgaa
     4261 gtctccaaga taggcatgct ccccgacgag gagagcagct ggctgaatcc cgacctgaag
     4321 gtctacaaga ccacggtcgt aattgatgga tcgcatgact ggctcaagcc gggcatgacc
     4381 gcgaaggtcg acatcctcat tgaagagttg cacgacgtcc tttacgtgcc gattcaggcc
     4441 gtgttcacgt ttgaaggaaa acgggtcacc tacctcgcgc aactcggccg cgaacctgaa
     4501 aggcgctacg tcgaaaccgg acagttcaac ggcgcctttg ttgaggtcaa gagcggtttg
     4561 aatgagggag acgtggtctt gctgattcag ccggagactc cgagcagaac cggtgagaag
     4621 ccggcggaga atatacccgg cgagctgccc agcgaggcgc ccgccgagac accggcgccg
     4681 gagaacgtgc aggcttcggc cgcggagtga gttgcatgca gggcaacgaa aagacagccc
     4741 gggataaggg cctgtccatg gcctgtgcca cggggccgct cgcgatgcag attctgtgcg
     4801 gtgcaggagg ccaatatggc ctcaagcagt aaatccacag cctgccaggc actccgcaat
     4861 gcattcggcg cgggccggat gaagctcacg atcaaactgg gactgaaaag tctctggctg
     4921 catcgtctgc gctcattact taccgtgctc ggcattgtct tcggtgtatg ctcggtgatc
     4981 gccatgctcg ccatcggcga aggagccagc tatgaagccc ag
//
LOCUS       DAOPBQ010000247         5013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14135_length_5013_cov_3.942719, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000247 DAOPBQ010000000
VERSION     DAOPBQ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14135_length_5013_cov_3.942719"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..2068
                     /locus_tag="PKW60_08850"
     CDS             383..2068
                     /locus_tag="PKW60_08850"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HPJ99578.1"
                     /translation="MPKYTIGLDYGTNSVRALVVNVANGAEVGTAVWNYAHGREGVIL
                     SSDPNLARQHPADYVKGAEATIAKALAAAKKSVKGFRPDQVIGIGVDTTGSTPLPVDE
                     TGQPLAFQKRFAKEPAAMAWLWKDHTSIEEAEEITGLARKTRPQFLAKCGGTYSSEWF
                     FSKILHCKRAHPKVYDAAFTWVECADWIPAMLTGNEAPGRYVACVCAAGHKAMYNDAW
                     GGYPDAEFLSKLDPALGTLRKRLNDKAYAFDTRAGRLSAAWAKKTGLPEGIPVATGAF
                     DAHLGAVGAGIQPGILARIVGTSSCDMAVASMAKPPADIPGLCGIVPGSILPGAIGLE
                     AGQSAVGDIFNWFVNYIQPGGPKNGSHEALTKEAARLAPGASGLIALDWNNGNRTILV
                     DQRLTGLLLGQTLYTTPAEVYRALIEATGFGALTIINRFEEYGVPIRRVLNCGGIAEK
                     NPLVMQIYADIMGRTMEISRSAQTCALGAAIAGAVVAGKKAGGYSSVAAAQKAMTGVK
                     TKKYTPNPKARRVYQELYPLYKDLHDALGTRAASGSLYHVMKRLLEIRAKARK"
     gene            complement(2093..3151)
                     /locus_tag="PKW60_08855"
     CDS             complement(2093..3151)
                     /locus_tag="PKW60_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HPJ99579.1"
                     /translation="MRKESLAFLKTLVTTPSPSGFEEEIQAVCKNYVKPYADKVYKDV
                     HGNQYAVRNPKGKLRVMLAGHVDEIGLMVNDIDENGFLGFVAIGGIDPSVLDGQRLVV
                     HGAAGPVPGVIGRTAIHLTEQEERGKPLKLHQLWADIGAKDQKDAQSVVAIGDPITID
                     AGFLALRDNKVVARAFDDRIGAFVVLEAMRLLAKRDLQCAVYCVTTVQEEIGLRGATT
                     STYGCEPHAGIAVDVDHATDYPFVDARRFGKQKINAGPVISRGPNINPIVNRQLIAAA
                     KKHKIPYQVSAAPRATGTDANAMQLSRNGVATGLIGIPNRYMHSPVELLSLTDAEHAA
                     QLIAEWVSALTPKTSFIP"
     gene            complement(3265..4497)
                     /locus_tag="PKW60_08860"
     CDS             complement(3265..4497)
                     /locus_tag="PKW60_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99580.1"
                     /translation="MLNKTDRAVIRELAGRVAGIAALPVQEEKRALWRRLNALDPVRP
                     MVMVDQVAWNEIQCEELALRCTDPECRVYETALRRSLYQWDHFPVDLVVEPFICVPKA
                     IENSGFGVEVLEDTVATDPANDIVGHRYINQFQTEEDLEKIQTPRLVHDTEETARRLG
                     VAHELFDGLLEVRPWGVDPYLSYWDPISTWMGVENALFALVERPEFMHRLVRRMMEGY
                     LAMLDQLETQGLLCEPQSLIHCTGAYSDELPAPGYDPEKPRTKDLWMFGLAQMFSTVS
                     PPMFKEFEVDYLAPICARFGLVYYGCCDPLDGKMNEVRMIPNVRKVSMSPWVDEERGA
                     EALQGEFVYSRKPNPALLADTTFDPARVRAHLTETRDVCERHGCPLEFILKDLSTLRY
                     EPERLDAWAEIAMDVAGA"
     gene            4688..>5013
                     /locus_tag="PKW60_08865"
     CDS             4688..>5013
                     /locus_tag="PKW60_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99581.1"
                     /translation="MMLVLFSLLGMSVEAPGTVWLDAYELQGGTVTLPSAATYSVWLW
                     ASGGGAAQVTLGGEAFQALENASSKGSFHWVKAGEAELPAGPVEIALEGNIASVALSS
                     DAGFHP"
BASE COUNT          932 a         1587 c         1584 g          910 t
ORIGIN      
        1 cgcgaggccc tctacaagag catggtcgag aaggcctacg aacaggaact ccagcgccaa
       61 ctgcctcaag tggacctccg cgtgcgccga aaagactgag cgcagcgccc ttatgctcat
      121 aagcgccgcc ggagacgatg cgcgcgggtt ctccggcttg cgggacgggg tccatgcggg
      181 cgcgccttgg attgcatcag cgacagcact cctgggacat cttcaaagcc gctccggcct
      241 gaccgcttcc tgatacgctt catttttcac gtactcccgg gtgattcacc gcagccggct
      301 tgcgtaatca gcgcgcggat aagtagactg aagcgcgccg gcctgcaggc cggcagggga
      361 gacgagcgaa aaggagcaac ccatgcccaa gtacaccatt ggtctggatt acggcacgaa
      421 ctccgtgcgc gcactggtcg tcaacgtcgc caacggcgcc gaggtcggta cggcggtctg
      481 gaattacgcc cacgggcgcg agggcgtgat tttgagcagc gatccgaacc tggcgcgaca
      541 gcatccggcg gattatgtga aaggcgcgga ggccacgata gccaaagccc tggcggccgc
      601 aaagaaatcg gtcaagggat tccgcccgga ccaggtgatc gggattgggg ttgatacgac
      661 cggcagcacg ccgttgccgg ttgatgagac gggccagccg ctggcgttcc agaaacggtt
      721 cgcgaaagaa cccgccgcca tggcgtggct gtggaaagac cacaccagca tcgaggaagc
      781 tgaagaaatc accggcctcg cacgcaaaac ccggccccaa tttctcgcca agtgcggcgg
      841 cacctacagc agcgaatggt tcttcagcaa gatcctgcat tgcaagaggg cccatccgaa
      901 agtctacgat gccgccttca cctgggtcga atgcgccgac tggatccccg ccatgctgac
      961 cggcaacgag gcgccgggcc gctatgtcgc ctgcgtctgc gcggccggcc acaaagccat
     1021 gtacaacgac gcctggggcg gctaccccga cgcggaattc ctctccaaac tcgatcccgc
     1081 gctcgggacg cttcgcaagc ggctgaacga caaggcctat gcgttcgaca cgcgcgcggg
     1141 cagactatcc gcggcctggg caaagaaaac gggcctgccg gaggggatcc ccgtggcgac
     1201 gggcgcgttt gacgcccatc tgggcgcggt gggcgcgggc atccagccgg gcatcctcgc
     1261 gcgcatcgtc ggcacaagct cctgcgacat ggccgtggct tccatggcca aaccgccggc
     1321 ggacatcccc gggctgtgcg gtattgtgcc gggcagcatt ctgccgggcg cgatcgggct
     1381 cgaagcgggc caatccgccg tcggcgacat tttcaactgg ttcgttaatt acatccagcc
     1441 gggcgggccg aaaaacgggt cccacgaggc gctcacgaaa gaagccgcgc ggctcgctcc
     1501 gggcgcgtcc gggctgatcg cgctcgactg gaacaacggc aaccgcacga tcctggtgga
     1561 tcagcgtctc acgggcctcc tgctcgggca gaccctctac accacccccg cggaggtcta
     1621 ccgcgccctg atcgaggcca cggggttcgg cgccttgacg atcatcaacc gcttcgagga
     1681 gtacggcgtc ccgatacggc gcgtgctcaa ttgcggcggc atcgccgaga aaaaccccct
     1741 cgtgatgcag atctacgccg atatcatggg acgcacgatg gagatctcgc gttccgcgca
     1801 gacctgcgcg ctgggcgccg cgatcgcggg cgccgtggtt gccggaaaga aggccggcgg
     1861 ctacagcagc gtggccgcgg cgcaaaaggc catgacgggc gtgaaaacga aaaaatacac
     1921 gccgaatccg aaggcccggc gcgtctacca ggagctgtat cccctctaca aagacctgca
     1981 cgatgctctg gggacacgcg ccgcgtcggg cagtctctat cacgtgatga agcgattgct
     2041 cgaaatccgc gcgaaagccc ggaaatagct gccttcagcg gataccgcgt cttcagggaa
     2101 tgaacgacgt cttcggcgtg agcgcgctga cccactcggc gatgagctgc gctgcatgct
     2161 cggcgtcggt cagcgacagc agctccaccg gagaatgcat gtagcggttg ggtatcccga
     2221 tcagcccggt ggccacgccg ttccggctga gctgcatcgc gttggcgtcg gtgcccgtgg
     2281 cgcgcggcgc ggcggacacc tggtagggaa tcttgtgctt tttcgccgcg gcgatgagct
     2341 gccggtttac gatagggttg atgttcggcc cgcgcgagat gaccggcccg gcgttgatct
     2401 tctgtttgcc gaaccggcgc gcatcgacaa aggggtagtc cgtggcgtgg tccacgtcga
     2461 ccgcaatgcc tgcatgaggc tcgcacccgt aggtgctggt cgtggccccg cgcaggccga
     2521 tttcttcctg cacggtcgtg acgcaataca ccgcgcactg gaggtcgcgt ttggccagga
     2581 gccgcatggc ctccagtacg acaaacgcgc cgatccggtc gtcaaatgcc cgcgcgacca
     2641 ctttgttgtc tcgcagggcg agaaatccgg cgtcgatggt gatgggatcg ccgatggcca
     2701 cgacgctctg cgcgtctttc tgatcttttg ctccaatatc ggcccagagc tggtgaagct
     2761 tcagcggttt cccacgttct tcctgctcgg tcaggtggat ggcggtccgg ccgatcacgc
     2821 ccggcaccgg tcccgccgcc ccatgcacca cgaggcgctg cccgtccaga accgacggat
     2881 cgatgcctcc gatggccaca aaacccagga acccgttttc gtcgatgtcg ttgaccatca
     2941 aaccgatctc gtcgacgtga cccgcgagca tcacccgcag ctttcccttc ggatttcgaa
     3001 ccgcgtactg gtttccgtga acgtctttgt agaccttgtc cgcgtagggt ttcacatagt
     3061 tcttgcagac ggcctggatt tcctcttcaa agcccgacgg gctgggcgtg gtcacgaggg
     3121 tcttaagaaa cgcgagcgat tctttgcgca tggatttgga tctccgttat gcagactgca
     3181 gtttcagcgg ccgcagaata ccagaagctc agcgggatcc gcaccgtgaa ggcggaaggc
     3241 gccggcgatg gcgggcagtt tccgtcaggc gccggcgacg tccatggcga tctcggccca
     3301 ggcgtcgagg cgctcgggct cgtagcggag cgtgctcaga tctttgagga taaactcgag
     3361 cgggcagccg tggcgttcgc aaacgtcgcg cgtttcagtg agatgggcgc gcacgcgggc
     3421 cgggtcgaat gttgtatccg caagcagggc gggattgggt ttccgtgaat agacgaattc
     3481 gccctggagc gcttccgcgc cacgctcttc atcgacccag gggctcatgg acaccttgcg
     3541 gacgttgggg atcatccgca cctcattcat tttgccatcg agcgggtcgc agcatccata
     3601 atagaccagg ccgaaccgcg cacagattgg cgccaggtaa tcgacctcaa attccttgaa
     3661 catcggagga gaaacggtcg aaaacatctg cgccaggccg aacatccaca gatctttcgt
     3721 gcgcggcttt tcggggtcat atccgggcgc aggcagctca tcgctgtacg cgccggtgca
     3781 atggatcagg ctctgcggtt cgcacagcag cccctgcgtc tccagctggt cgagcatggc
     3841 cagatacccc tccatcatcc ggcgcaccag ccggtgcatg aactcgggcc gttccacgag
     3901 cgcgaacagg gcattttcga cgcccatcca cgtgctgatg gggtcccagt aggagagata
     3961 cgggtcaacg ccccagggac gcacctcgag caggccgtca aacagctcgt gggcaacgcc
     4021 cagccggcgc gcggtctcct ccgtgtcgtg cacgaggcgg ggcgtctgga tcttttcgag
     4081 atcctcttcc gtctggaact ggttgatgta gcggtggccc acaatatcat tggccggatc
     4141 ggtggcgacg gtgtcctcca gaacctcgac gccgaaaccg gaattctcaa tcgccttggg
     4201 cacgcaaatg aatggctcca ccaccaggtc gacggggaaa tggtcccatt ggtacaggct
     4261 gcggcgcagc gcggtttcat agactcggca ttcgggatcg gtgcagcgaa gggccagttc
     4321 ctcgcactgg atctcgttcc aggccacctg atccaccatc accatgggcc ggaccgggtc
     4381 aagcgcgttc aacctgcgcc acaacgcgcg tttctcttcc tgcactggca gtgcggcgat
     4441 cccggccacc cggcccgcca actctcgaat cacggccctg tcggtcttgt tcagcatatg
     4501 catgctcctc tgcgcacagc gcgctgagca taccacagcc cccctgattt cagcgctacg
     4561 atgatctccg caatccgcct tttcccgcca ctggcggctg catgcccgga cggtccctct
     4621 gcgttttgat tttaccgcgt ttcgcgatat gctcaactga aactcatttg tcagggaggt
     4681 tgcagaaatg atgctggtac tgttttcgtt gttgggcatg agcgttgaag cgccgggaac
     4741 cgtctggctg gatgcctatg agctgcaggg cgggaccgtg accctgccaa gcgcggcaac
     4801 gtactctgtg tggctgtggg ccagcggagg aggcgcggct caggtgaccc tgggcggtga
     4861 agcgtttcag gcgctggaaa acgcgtccag caaaggctcc ttccattggg tgaaggccgg
     4921 cgaggccgag ttgcccgcgg gtccggtcga aattgcgctg gaaggcaata tcgcttccgt
     4981 agcgctgagc tcggatgccg gcttccatcc gga
//
LOCUS       DAOPBQ010000248         5004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14165_length_5004_cov_2.987876, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000248 DAOPBQ010000000
VERSION     DAOPBQ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14165_length_5004_cov_2.987876"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PKW60_08870"
     CDS             <1..889
                     /locus_tag="PKW60_08870"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HPJ99582.1"
                     /translation="HAAQIRALKGAGCPDILMSQKHPRRAGSPYRIRQANPPGKVYEM
                     TNPYIRDVQAFGRMIRRIERAGQILLTLWVAVLIALGIASPQPYMRAWELVLLQIVAG
                     RAASVSKGVAMGFPKLFLLAHCTLQDIIVLLLLYPLLVAGYRYAVEWRFIGPAIANVR
                     ATAERHKSKIAPFGSIGLVLFVLFPFWSTGALAGGVVGYLLGMRTRWVFTSVIIGDFL
                     AVTLWIWFFDRMARFSEALSSRMWLFILLGVVLTAAVGRIIAIRHKMKPARAGSETAK
                     LSGTQNERPGFPEKTGPDS"
     gene            complement(908..2239)
                     /locus_tag="PKW60_08875"
     CDS             complement(908..2239)
                     /locus_tag="PKW60_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HPJ99583.1"
                     /translation="MSKGTRINLCIMMFLEFFVWGAWYVPMGVYLGALKFPGTAIGNA
                     YGTTALAAIVSPFFVGMIADRFFSAERVLGAMHILGGIVLFAVSRIAEPGLFFWVLLI
                     YTVTYMPTLALVNAVAFQQLDNIEKQFPAIRVFGTLGWIVAGLLIGFLEVNSGGVRWH
                     FAEPFGIPINFTIPLAGGEGEWIAAPATAIPMVIAALAAIVLGVFSFFLPHTPPKSKG
                     QSVKVRDILGLDALGLLKDRSFAVFAISSFLICIPLSAYYAFANPFLDEMGMVNATAK
                     MTLGQMSEFFFMLVMPFFFARLGVKKMLLVGMAAWVGRYLLFTFGDNGALVFMYYGGI
                     LLHGICYDFFFVTGQIYVDNEAPKEVRASAQGFIAMITYGVGMYIGSILAGIIVQHYQ
                     IMEGEEIAGHNWPPVWKIMTLMAVVVAVAFAVLFKDKGTPEAVAEEASTEA"
     gene            complement(2833..>5004)
                     /locus_tag="PKW60_08880"
     CDS             complement(2833..>5004)
                     /locus_tag="PKW60_08880"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPJ99584.1"
                     /translation="QADAEAALKGGGLVVGIVTQEYNATIPAGRVISQSPAAGTSVKH
                     GSAVSLAVSKGPEPQPVAVPDVVGRPLAEAEAALISAGLVVGKVTEAQHDTIPFGRVI
                     SQSPAAGTNTTSGSAVDLVVSHHRPRIRISTIEELQRIGNDTDYPLNGRYILTKDIDA
                     SATATWNGGKGFSPIGDRNAPFMGVFQGDGCVISNLVINQPGSGYMGLFRYVGQDAEI
                     WSLTLADGTITGDRFVGSLAGYNEGGIYNCSAEDTEVHGDEIVGGLIGVNSGDVQISW
                     VSDSVVSGVDRVGGFVGRNEPGAYIRKSLVQNSSISGDTNVGGFVGSSYSGHIEDSGV
                     QDSIISGDEYVGGFAGRNEIDAYIRDSWVQDSSISGENYVGGLVGEHSSYNFIGGSWV
                     QDSIVSGESYVGGLVGDSKGHAGLSRVQDSSISGNEYVGGLVGINHYGEVFTSSVQGS
                     SISGESDVGGLVGWTGQYTYVGSSFVSDSNITGAESVGGLVGTNAGGKTHYSVVRDSD
                     VRAGLGARTYFGGMVGESWGEVEGCTSDCRVLCDESGPSQNANLIDNVGGLIGFARDC
                     KIVKCASLGPVAGRSAVGGLVGSMHGATSIKYCYATGDVHASSNSGGLTGSGEGTCTV
                     TECYSVGFVDCGGDSTCGALAGVSDSGGFPAVTASFWNKETSGFLTSFGGGEGRTTSE
                     LKQKATFTAAGWDFVDEWSTWLIDEGLSYPYFRFTTYPWYP"
BASE COUNT         1080 a         1560 c         1358 g         1006 t
ORIGIN      
        1 acacgctgcg cagatacgcg cgttgaaggg tgctggctgc ccggatatac tgatgtccca
       61 gaaacacccc cgccgcgcgg gcagccctta ccgaatccgc caggcaaacc cgccgggcaa
      121 ggtatacgaa atgaccaacc catatatccg cgacgtgcag gccttcgggc gcatgatccg
      181 ccggatcgaa cgtgccggcc agatactgct taccctctgg gtggcagtcc tcattgcgct
      241 ggggattgcc tcccctcagc cgtatatgcg cgcctgggag ctcgtgctgc ttcagatcgt
      301 tgccggccgg gccgccagcg taagcaaggg cgtagccatg ggatttccca agctgtttct
      361 gctggcgcac tgcacgttgc aggatatcat tgtcctgttg ctgttgtatc cgctgttggt
      421 cgccggatac cgctatgccg tggagtggcg cttcattggg ccggcgatcg cgaatgtccg
      481 agcgacggcg gagcgccaca agagcaagat tgcgccgttt ggctcgatcg gcctcgttct
      541 ttttgtattg tttcccttct ggagcaccgg cgcgctcgcg ggaggcgtgg tcgggtacct
      601 gcttggcatg cggacccgct gggtcttcac ctcggtgatc atcggcgatt ttctcgctgt
      661 aaccctgtgg atctggtttt tcgaccggat ggcccggttc tcggaagcgc tgagcagccg
      721 catgtggctg ttcattctgc tgggcgtggt gctcacagcg gctgtaggcc gcatcatcgc
      781 catccggcac aaaatgaaac cggccagggc gggcagcgag actgcaaaac tcagcgggac
      841 gcaaaatgaa cggcccggct ttcctgagaa aaccgggccg gattcgtgaa aaccgcgagg
      901 gattgactca ggcctctgta gacgcctctt ccgctaccgc ttctggcgtg cccttgtctt
      961 tgaagagaac agcgaatgcc accgcaacaa cgactgccat aagggtcatg attttccaca
     1021 ccggcggcca gttgtggccc gcgatctcct cgccctccat gatctggtag tgctgaacaa
     1081 ttattccggc cagaatcgag ccgatgtaca tgcccacgcc gtaggtgatc atggcgatga
     1141 agccctgtgc actggcccgg acctccttgg gcgcctcgtt gtcgacgtag atttgaccgg
     1201 tcacgaagaa gaagtcgtag cagatgccgt gcaggaggat gcctccgtaa tacatgaaca
     1261 ccagcgcgcc gttgtcgccg aatgtgaaca gcaggtaccg gccgacccag gccgccatac
     1321 ccaccaggag catcttcttc acgcccagcc gcgcgaagaa aaacggcatc accagcatga
     1381 agaaaaactc cgacatctgg ccgagggtca ttttggccgt tgcattcacc atgcccatct
     1441 catccaggaa tggattcgca aacgcgtagt acgctgaaag cgggatgcag atgaggaagg
     1501 agctgatggc aaacaccgca aacgagcggt ctttcagcag gccgagggcg tcgaggccga
     1561 gaatgtcgcg gactttgact gactgtcctt tcgacttggg gggcgtgtgc ggcaggaaga
     1621 aactgaacac gcccagcacg atagcggcca gcgcggcaat gaccatgggg atggctgtag
     1681 ccggcgcggc aatccattcg ccttcgccgc cggccagcgg gatcgtgaaa ttgatgggga
     1741 ttccaaacgg ctcggcaaaa tgccagcgca ccccgccgct gttcacctcc aggaaaccga
     1801 tcagtaaccc ggccacgatc cagcccaggg tgccgaatac ccggatcgcc gggaactgct
     1861 tttcaatgtt gtcgagctgc tggaacgcca ccgcattcac cagcgcgagg gtcggcatgt
     1921 acgtcacggt gtagatcaac aagacccaga agaacaggcc gggctctgca atgcgtgaca
     1981 ccgcgaacag cacgatgcct cccagaatgt gcatggcgcc caggacccgc tctgcggaaa
     2041 agaaccggtc ggcgatcatg cccacgaaga acggcgagac gatcgcagcc agggcggtag
     2101 tgccgtatgc attgccgatg gccgtgccgg ggaatttgag cgcccccagg tacaccccca
     2161 tgggaacgta ccaggctccc cagacaaaga attccagaaa catcatgatg cacagattga
     2221 tgcgggtccc tttactcatc tcgaatgcct ccttctgcga tggcagcggg ttggaacgcg
     2281 caggaagggg ggcagcagga gccgaccatc acaaatctgg aaccccctcg gggaaacggc
     2341 cacccccatc gtgtctctgc cgttcgaatc gcatcctagt aaaaaggccg caccgggtca
     2401 agtctgcgaa gctgctcaat cgtgagatat tctgaagccg tgcgcctatt cataattcca
     2461 agtgcacggg tttttcattt gatggtttgg acgccttttt tcgtgttggt gggtgtggag
     2521 cgggacatgg atgaaagaag cacgcatcgc agagtcggtt gggtccactg ggaatcatca
     2581 aaatcacaga caacccaaag gagaactccc agtgggcaaa cccacatcat gcaacgtata
     2641 aatgaggtga aattcaggcg tattcccacc cgggggtgtt tccggcggga attccctgaa
     2701 aaaagagctg ccctgcctct actatcgggc gcaaattacg ttgaaacagc tgcggctata
     2761 cctgtccttt cgggatcgct caaaggcgat acctccaacc ccgaaaagcc tgcctccgtc
     2821 aatcatgtca aatcatggat accatggata cgtggtgaag cggaaatagg gatacgatag
     2881 cccctcgtcg atgagccagg tggaccactc gtcgacgaaa tcccaacctg ccgcggtaaa
     2941 ggtggccttc tgcttcagct cagaggtggt tcggccttca ccgccaccaa aggaagtgag
     3001 gaagccgctg gtctccttat tccaaaacga ggctgttacg gctgggaaac cgcccgaatc
     3061 agaaacccct gcaagcgcgc cgcaggtgct gtctcctcca caatcgacaa agccaacgga
     3121 atagcactcc gtgacggtgc aggtgccctc tccagagccg gtcagcccac cactgttgga
     3181 gctggcatgt acatcgccag tcgcataaca gtatttgatg ctggtggctc cgtgcatgct
     3241 tcccactaaa cctccgactg ctgatctgcc cgcaacaggt ccgagtgagg cacacttgac
     3301 aatcttgcag tcccgggcga atccaatcaa tccgccgacg ttatcgatga gattggcgtt
     3361 ttgcgagggt ccagattcgt cgcaaagcac cctgcaatcc gaggtgcagc cttcaacctc
     3421 cccccaactc tcgccgacca tgcctccaaa gtaggtccga gcaccaaggc cagccctgac
     3481 gtcggagtcc cttaccacgg agtaatgtgt tttgccgcca gcattggttc ccacgagccc
     3541 gccgacactt tcggcccctg tgatgttgga gtccgataca aatgaactac ctacataggt
     3601 gtattggcca gtccatccca caagcccgcc gacatctgat tctccgctga tgctggagcc
     3661 ctgcaccgag gaggtaaata cctcgccata atggttgatc cccacgagcc cgccaacata
     3721 ctcatttcca ctgatgctgg aatcctgtac ccgggagagc cctgcatggc ccttactgtc
     3781 ccccacgagc ccgcctacat aagactcccc actgactatg gagtcctgta cccatgaacc
     3841 ccctatgaag ttataacttg aatgctcccc cacgagtccg ccgacataat tttctccact
     3901 gatgctggaa tcctgcaccc aggagtccct tatgtaggcg tcgatctcat ttctccccgc
     3961 gaacccgccg acatactcat ccccactgat gatggagtcc tgcaccccgg agtcctctat
     4021 gtggccgcta taactggatc ccacgaagcc cccgacattt gtgtccccac tgatgctgga
     4081 gttctgcacc aaggatttcc ttatgtaggc gccgggctca tttctcccca caaacccgcc
     4141 gactctgtct accccgctga cgacggagtc cgacacccac gagatttgta cgtcgccact
     4201 attgactcca attaagccgc caacgatctc gtccccgtgg acctcagtgt cttcagcact
     4261 acaattgtaa atccccccct cattgtaacc ggcaaggctg cccacaaagc ggtctccggt
     4321 tatggttccg tcggccaggg ttagagacca tatctccgca tcctgaccga cgtaccggaa
     4381 caggcccatg taaccgctgc ccggctggtt gatcacgaga tttgaaatca cacacccatc
     4441 tccctggaac acacccataa aaggagcatt acgatcccct atcgggctga agcccttgcc
     4501 accattccag gtggcggtgg ccgaggcatc gatatctttt gttaaaatgt aacgcccatt
     4561 aagcgggtag tctgtatcat taccgatgcg ctgaagctcc tcgatggttg aaattctgat
     4621 ccgtgggcgg tgatgcgaca ctaccaggtc gacagcactt ccactggtgg tgtttgtacc
     4681 cgcagccgga ctctggctga tcactctgcc gaaagggatc gtgtcgtgct gggcctcagt
     4741 caccttaccc acaaccaagc ccgcacttat cagggcggcc tccgcctccg ccagcggcct
     4801 gcccacgaca tccggcaccg cgacgggttg gggctcaggg cccttagaca ccgctaaact
     4861 cactgcagac ccgtgcttta cggacgtgcc agcagccggg ctctggctga tcaccctgcc
     4921 cgcagggatc gtggcgttat attcctgggt cacaatgccc acaaccaggc cgccgccctt
     4981 cagggctgcc tccgcatctg cctg
//
LOCUS       DAOPBQ010000249         5003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14166_length_5003_cov_4.246968, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000249 DAOPBQ010000000
VERSION     DAOPBQ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14166_length_5003_cov_4.246968"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..2140)
                     /locus_tag="PKW60_08885"
     CDS             complement(59..2140)
                     /locus_tag="PKW60_08885"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPJ99585.1"
                     /translation="MCSSLVIGLTSLVLAAAIPGVPPDAVLLPLFSLEQELPAFLIQN
                     HTTAKLAVYDETPLMDVSFQKADWPNVYFKAPDSPWDWSGALGVAIDVYNPESTPVQV
                     AMRIDNAGADGLHDCITINALALPGKWTPVFAPLPSADTDRFWGMRGIPLFGPVGTGK
                     GIDPSRITAFQLFLPLPERPHQLFLSNFRLIRTSVPIGEAISFPFIDEFGQYQHADWP
                     GKLHDAREWPQQIEAEAADLSAEIPQWDEFGGWAAGPQREATGWFRTEQIDGTWWLIT
                     PTGHLFFSTGMDCVSTWNPTFVEGRDEWFEGLPPEDHPQFGSCYHRVDNAHSGADVIG
                     GKGRTFDFYRANLMRKYGESWEKRWRETSYARLKSWGFNTVGNWSQSDVLDNSPLPFV
                     ISGAIHNVRRVEGGGGYWAKMTDPFDPEFPEKVESSLRGTLQACKDNPRCIGYFVDNE
                     LAWEAVEQGSLSSPPEQPCRQAQIAMLTDKYGTIDALNAAWETSATDWVALRAPEEPN
                     AACTADLDEFVRAFALRYFHAINDVIKRHAPNQLYLGCRFSTAPRTVVEAADDVCDIV
                     SFNLYQTRIDCSRWTGANALSAPIIIGEFHFGALDRGMFHTGLVPTGSQEARAQGYID
                     YVRSVADCPAFVGCHWFQYVDEPTTGRWFDGENYNIGLVTIVDSPYPEMVAAARRVHA
                     EMYRRRFERAR"
     gene            complement(2192..3556)
                     /locus_tag="PKW60_08890"
     CDS             complement(2192..3556)
                     /locus_tag="PKW60_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPJ99586.1"
                     /translation="MYSSRRDFFKTAGINAAMLAVPAGAAPQIQTKHPEIAWSRKVPL
                     RYEADVAVIGGGIAGVCAALAAARSGASVMLVERFAVCGGNATIGGVASFCGETSGQG
                     EAFDAIIAGLEAWNAIAPYQPYEEREARVFDHEILAIVLQELLLRRNVRLLLHTGFVD
                     ALEKRGRVSECVVCGTSGPEALRARQFIDCTGEAQLVSMAGFDTVKGRPEDGYSLPMS
                     MMYFVRHVDDEDARPQLPDGWFHPVRTKDELPMLSIWPNGPRANALKLKIPMFDASDT
                     ESLTAAEIHARRRMMEVLDYYQRVEKRPWLLDHCSPRIGIREGRRMLGDYVLQLEDLR
                     AGRKFDDAIARGVFYLDGHKPDDEKRTYILPEEERWVPPYQIPFRSLLPRGAKNLLGA
                     GRCFSADQLALSSARVMTTCSMMGQAAGIAAAMAVEAGCDPRDLDYSLIRKTVTERGA
                     RLDV"
     gene            complement(3719..4717)
                     /locus_tag="PKW60_08895"
     CDS             complement(3719..4717)
                     /locus_tag="PKW60_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPJ99587.1"
                     /translation="MTTRMNHVERFRAVMNCQAVDRLPRWEWATWWDKTIDRWHGEGL
                     PADLTDVFEIHDYFGLDPYKQFWFSPRRPGIEKLHPGIDGGVSGMDDYRRFLPYLYPD
                     HTAAIESMRPWAARQARGDVVVWITLEGFFWLPRTLMGFMKLMYAFIEQPELIHRMNE
                     DLLRFNLDVLDQVEKVCVPSFLTLAEDMSFNNGPMIGEALFEEFVAPYYRRLLARVKE
                     QNILTIVDTDGDVTLMTPWLKRAGIDGVLPLERQAGVDGAAIRQLCPDFVLIGHYDKM
                     VMPSGEQAMRGEFERLVPLMKSGGFIPSVDHQTPPGVSLDQYRVYLRLLHEYTALA"
BASE COUNT          921 a         1541 c         1609 g          932 t
ORIGIN      
        1 gcgcagccac accccaccgc gacggattca ctaattgccg ccccgcgggg tttagaggtc
       61 agcgggcgcg ctcaaaacgg cgccggtaca tttcggcatg gacccggcgc gcggcggcga
      121 ccatttccgg atacggtgaa tctacgatcg taaccaggcc gatattgtag ttttcaccgt
      181 caaaccagcg gcccgtggtg ggctcatcga cgtactgaaa ccaatggcat ccgacgaacg
      241 cggggcagtc ggcgacgctg cgcacgtagt cgatataacc ttgtgcgcgg gcttcctggc
      301 ttcccgtggg gacaagcccc gtgtgaaaca tgccgcggtc aagtgcgccg aaatgaaatt
      361 cgccgatgat gatgggcgcg ctcagcgcgt tggcgcccgt ccagcggctg caatcgatcc
      421 gggtctggta gagattaaag ctgacgatgt cgcacacgtc atccgctgct tcgaccaccg
      481 tgcggggcgc ggtagaaaac cggcagccga ggtagagctg gttgggcgcg tggcgtttga
      541 tcacatcgtt gatcgcgtgg aagtagcgca gggcaaacgc acgcacaaac tcatcgagat
      601 cggccgtgca ggcagcattg ggttcttcag gcgcgcggag ggcaacccag tccgtggcgg
      661 acgtctccca ggcggcattg agcgcatcga tcgtgccgta tttgtccgtc agcatggcga
      721 tctgcgcctg ccggcagggt tgctccggag ggctgctcag cgacccctgt tcgacggctt
      781 cccaggcaag ttcgttgtcg acaaaatagc cgatgcagcg gggattgtct ttgcaggcct
      841 gaagcgtgcc ccgcagggat gactcaactt tctccggaaa ctcggggtcg aagggatcag
      901 tcattttagc ccagtacccg ccgccgcctt cgacacgcct cacgttatgg atggcgccgc
      961 tgatcacaaa cggcaggggg ctgttatcga gaacgtcgct ctgagaccag ttcccgacgg
     1021 tgttgaagcc ccaggacttc agccgggcgt agctggtttc gcgccagcgt ttctcccagg
     1081 attcgccgta tttgcgcatg agattggccc ggtagaagtc gaatgtcctg cctttcccgc
     1141 cgatgacgtc ggcgccgctg tgggcgttgt cgacccggtg gtaacaggag ccgaattgcg
     1201 gatggtcttc cgggggaagg ccttcgaacc actcgtcgcg gccttccacg aaggtggggt
     1261 tccaggtgct cacgcaatcc atgccggtgg agaagaacag atggcccgtg ggggtgatca
     1321 gccaccacgt gccgtcgatc tgctcggtgc gaaaccatcc agtagcttcc cgctgaggcc
     1381 ccgcggccca gccgccgaat tcgtcccact gcggtatttc ggcgctcaga tcggccgctt
     1441 ccgcctcgat ctgctgcggc cattcccggg cgtcatggag tttgccgggc cagtcggcat
     1501 gctggtactg gccgaattcg tcaataaacg ggaaggaaat tgcttcgcca atggggacag
     1561 aggtccgtat caggcggaag ttcgacaaaa agagctggtg cggacgttcc ggaagcggca
     1621 gaaagagctg aaacgccgta atgcgggaag ggtcgatgcc tttgccggtc cccacggggc
     1681 caaagagggg aatgccgcgc atgccccaga aacggtccgt atcagcagac ggcagcgggg
     1741 caaagaccgg ggtccatttc cccggcaggg cgagcgcgtt gatggtgatg cagtcgtgaa
     1801 gaccgtcggc gcccgcgttg tcgatccgca tggccacctg aaccggcgtg gattcgggat
     1861 tgtagacatc tatggcgaca ccgagcgcgc cgctccagtc ccacgggctg tccggcgcct
     1921 tgaaatagac gttcggccag tccgcctttt gaaacgagac gtccattaat ggtgtctcgt
     1981 cgtacacggc cagtttggcc gttgtatgat tctggatgag gaaagcgggg agttcctgct
     2041 ccagggaaaa caggggaaga aggaccgcat cgggcggtac gcccgggatg gctgcggcca
     2101 ataccagcga cgtcagtcca ataacaagac tgctgcacat gaactcgttc ctcctgtgcc
     2161 ggctctggca tctccggctc tattgttcgt attacacatc aaggcgcgcg ccgcgctccg
     2221 tgacggtttt gcggatgagg ctgtaatcga ggtcgcgcgg gtcgcacccg gcctcgacgg
     2281 ccatcgcggc ggcaataccg gcagcctggc ccatcatcga gcaggtggtc atgacgcgag
     2341 ccgacgacag ggccaactgg tcggctgaga aacagcgccc cgcgcccagc aggtttttcg
     2401 cgccgcgagg cagcagcgac cggaaaggta tctggtaggg cggcacccag cgctcctctt
     2461 ccggcaggat gtaggtgcgc ttttcatcgt caggcttgtg tccgtcgagg tagaacacgc
     2521 ctcgtgcgat ggcatcgtcg aatttccgcc ctgcgcgcag gtcttcgagc tggagcacgt
     2581 aatcgcccag catgcggcgg ccctcgcgaa tgccgatgcg cggagagcag tgatccagca
     2641 gccagggccg tttctcgacg cgctggtaat agtcgaggac ctccatcatc cggcggcggg
     2701 cgtggatctc cgcggcagtg agcgactccg tgtcgcttgc atcaaacatg gggattttca
     2761 gtttcagggc gtttgcgcgc gggccgttgg gccagatgct gagcatcggc agctcgtcct
     2821 tcgtgcggac cgggtgaaac catccgtccg gcagttgagg ccgcgcgtct tcgtcatcga
     2881 cgtggcgcac gaaatacatc atcgacatcg ggagcgaata tccgtcctca ggccgccctt
     2941 tcaccgtatc gaatcccgcc atgcttacga gctgggcttc gccggtgcaa tcgatgaatt
     3001 gccttgcgcg cagcgcctcc gggccggatg tgccgcagac cacgcattcc gaaacgcggc
     3061 cgcgtttttc cagcgcatcc acgaaaccgg tatgcagcag cagtctcacg ttacgccgca
     3121 gaaggagttc ctgcaatacg atggccagga tctcgtgatc gaagacccgg gcttctctct
     3181 cttcgtaggg ctggtagggc gcgatggcat tccacgcctc caggccggcg atgatggcgt
     3241 cgaacgcctc gccctgcccg gaggtctcgc cgcagaagga agcgaccccg ccgattgtgg
     3301 cgttgccgcc gcagaccgca aaccgttcga ccagcatgac gcttgcgccg gagcgtgcag
     3361 cggccagcgc ggcgcacacg ccggcgatgc ctccgccgat caccgccacg tcggcctcgt
     3421 agcgcagagg tacctttcgg ctccaggcga tctccgggtg ttttgtctga atctggggcg
     3481 ccgcaccggc tggaaccgcg agcatcgccg cgttgatgcc cgccgtcttg aaaaaatccc
     3541 ggcgtgacga atacatgatt tcctccagaa gcggcgcgcc cgggccgctg tcgttttccg
     3601 cagtatagca actgcctgca tcgcggcggc aaactggaac aaccgcaggc tcaggctcgg
     3661 acttgccctc gccagtctcc cgcctggcgt tactcccgcg gcgcttcagc ggccgaattt
     3721 acgcgagggc cgtgtattcg tgcaacaggc ggagatacac gcgatactga tcgagggata
     3781 cgccgggcgg cgtctggtgg tcaacgctcg ggatgaatcc gccgcttttc atcagcggga
     3841 ccaggcgctc gaactcgccg cgcatggcct gctctcccga aggcatgacc atcttgtcgt
     3901 agtggccgat caggacaaaa tcgggacaaa gctgccgaat cgcggcgccg tcgaccccgg
     3961 cctggcgctc cagcggcagc acgccgtcga tgcccgcccg tttcaaccag ggcgtcatca
     4021 gcgtcacatc gccgtcggtg tctacaatgg tgaggatatt ctgctccttg acccgggcca
     4081 gcaggcggcg gtaatacggc gcgacgaact cctcgaacag tgcctcgccg atcatgggcc
     4141 cgttgttgaa cgacatgtct tcggcaaggg tcagaaacga aggcacgcag actttctcaa
     4201 cctggtcgag cacgtcaaga ttgaagcgca gcaggtcttc attcatgcgg tggatgagct
     4261 cgggctgctc aatgaacgca tacatgagtt tcatgaagcc catcagggtc cggggcagcc
     4321 agaaaaaccc ttcgagcgtt atccagacca ccacgtcgcc ccgtgcctgg cgcgcggccc
     4381 acggccgcat cgattcgatg gcggccgtgt ggtccgggta cagatacggc aggaaccgcc
     4441 ggtagtcatc catccccgac acgccgccgt cgataccggg gtgcagtttc tcgatgcctg
     4501 gccggcgggg gctgaaccag aactgtttat acgggtcgag tccaaaatag tcgtggattt
     4561 cgaagacatc cgtcagatca gcgggcaatc cctcgccgtg ccagcggtcg atggtcttgt
     4621 cccaccacgt cgcccactcc cagcggggca ggcggtcgac ggcctgacag ttcatcacgg
     4681 cgcggaaccg ctcaacgtga ttcatgcgtg tggtcatgga ctggactcct tgcagctcag
     4741 cggccctgag ggtagcgtat cgggcagaga ttccacaatc cgccccggac gcggttgctg
     4801 cagcgttcgg cgtcgcctat actgatgctc cgtgctgagg ccggagaagg agcgatctgt
     4861 gcgcaaagcg cttgggtata gcgtgctcgg actggcgttc gggctggtgc tgcttgcagg
     4921 aatcggctgc gagggcggcg gtctggccat ccagatgccc gccccggatt caggcgatca
     4981 acccggcggc ggaggcgggg gag
//
LOCUS       DAOPBQ010000250         4976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14242_length_4976_cov_3.770169, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000250 DAOPBQ010000000
VERSION     DAOPBQ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14242_length_4976_cov_3.770169"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKW60_08900"
     CDS             <1..287
                     /locus_tag="PKW60_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99588.1"
                     /translation="QMLELILDAHGTRILRKGIRVQHTVAEMKERCSDDVALAAFLET
                     LPPDAVFDHETVNAHLPAAMTAHNRAEALFAYLRELRTSGEALLEQQSLE"
     gene            292..1986
                     /locus_tag="PKW60_08905"
     CDS             292..1986
                     /locus_tag="PKW60_08905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPJ99589.1"
                     /translation="MRILVAQMARMGDMLQNSTLTRTLRQRYPGAHITAMVRPLARAI
                     AERNPDINDIMLYEEDATFGDLRSSDSERLLRAYRSAAALVDDLNARQFDVIYNCTLS
                     TASAALLKMVHGPQIIGAHLSDDWLFVLRGPWTNYFFTSVHQRVYSDINISDVFRNFM
                     QDAPSVPGLSFAVTDDDRREVREILAEHGISDRDFVVCLQLGASDSSKRWPVAQFAEL
                     ARRLADEKNARILLLGVESESPLGEAFEAQAPGLAVHLFGKTTIPQLAALLESARALV
                     TNDTGTMHLATAVRCPVLLLSVGYVHFRETGPYGEGHLAIERRKDYLAPMSLQDTATD
                     DRNAIPAGPVMDLFELLLTPSHDGSIPSLSDSPERALLDVYQSRFAPDGFLEWYPVIC
                     RPMVEADFLRIAYRNTWLAHLCPHIPRETLQDGIQRLVSCFAPTQMALLDSWCEHWGA
                     VFLQLEQLARLGHEKTEQLRACLRQGRNITMAREIVGELMRLDEDTRLFAELNEACRP
                     LISIARFQRDNLEGADPAPLAEMTQEIYQDLLERAALMQLYFGVIRDAVAATHAPG"
     gene            complement(1992..3659)
                     /gene="recN"
                     /locus_tag="PKW60_08910"
     CDS             complement(1992..3659)
                     /gene="recN"
                     /locus_tag="PKW60_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPJ99590.1"
                     /translation="MLERLHIQNYALIDEIELEFGPGFNVLTGETGAGKSIIVGALNL
                     VLGARASSEGVRDGADRARVDAVFRIEDPARDLAAALDASGILLDDGVLILSRVITAD
                     GRSRAFAAGVPVPLTVLGALGDELVDLHGQHEHQSLLKPSRQLNLLDGFAGAESNVIT
                     VAEAVSALRGAERELAVLEVDDRERARRIEFLQHEAEEIEKAALEPGEEEERRERRDL
                     LPHAERIFELSSQAYASLYEHEEGAVVDALAAAIGNVEELARINSRFAPFVERLADAR
                     EKIEEVAAELREFTTEVDFDAEELEGINARLTLISALKRKYGASIDAVLDYCRRARAE
                     VAAFEQRDERIAQLQRQRDALLEKARDLAGVLSVLRKKAACTLDKQIAAALAELGMKG
                     GRFETRFEHGELSPTGIDRVEFLLAANPGEALKPLRQVASGGEISRVMLALKSVFANA
                     DTIPTLIFDEIDAGVGGAVAKKVAAKLGELADSHQTICISHLPQIAAAAETHFHVAKM
                     IQKKRTVTSVARIADEERVRELARLLDGSVSEVSLEHARSLLSPPAR"
     gene            complement(3693..4433)
                     /locus_tag="PKW60_08915"
     CDS             complement(3693..4433)
                     /locus_tag="PKW60_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPJ99591.1"
                     /translation="MEKERLDILVQRQAGVSRSKARGLIHTGQVYGPGSRKLDKPGMR
                     LDVSTPLEVRDESRYVSRGGDKLEAAFAAFTFDVAGRVAIDVGASTGGFTDCLLQHGA
                     AYVYAVDVGYGQLAWRLRQDPRVCVMERTNIRHVAAEALPRLPEVFTADCSFISLRLV
                     LPVLQTLLRKPAEGVVLIKPQFEAGKTLVGKGGVIRDEAVREQTVQDVLAAAEAFGFE
                     TGGVIPSPLKGPKGNQEYLAYLRLPAIE"
     gene            complement(4512..>4976)
                     /locus_tag="PKW60_08920"
     CDS             complement(4512..>4976)
                     /locus_tag="PKW60_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99592.1"
                     /translation="ALSFHKSPLSLAGISWVPESEEFLLGGLKVKLEIAMFLSTALIT
                     YAMMELTSWLAPAHSDELERAQAFLARLDVPIGKLAEDQEHDDAGGATIISPFRIVGV
                     CVAVIGVMMLALVPWIHEATPRMLDIILGSILLLAGVFVAWSSKRDLIGSKS"
BASE COUNT          940 a         1644 c         1430 g          962 t
ORIGIN      
        1 cacagatgct cgagctgatc ctcgacgcac acggcacgcg catccttcgc aagggtattc
       61 gcgtgcagca tacagtcgcc gaaatgaaag aacgctgctc agacgacgtt gcactggcgg
      121 ccttcctgga aaccctgcca cccgatgccg tgttcgatca tgaaaccgta aacgcgcacc
      181 tgcctgcagc catgacggcc cataaccgcg ccgaagcgct gttcgcctat ctgcgcgagc
      241 tgcgcacttc cggcgaagca ttgctcgagc agcagagtct ggagtaacgg catgcgtatc
      301 ctggtggctc agatggcccg tatgggagat atgcttcaga attcaacctt gacccgcacg
      361 ctgcgccagc gttatcccgg cgcgcacatt accgcgatgg tccggcctct ggcgcgcgcc
      421 attgccgaac gcaatcccga tatcaacgac atcatgcttt acgaagaaga tgcgacgttc
      481 ggcgatttac gcagcagcga ctccgagcga ctgctccggg cataccggag cgccgcggca
      541 ctggttgacg acctgaatgc acgccagttc gatgtgatct acaactgcac gctcagcacc
      601 gcctcagctg cgttgctgaa gatggtccac ggcccacaga tcattggcgc acatctcagt
      661 gatgactggc tctttgtgtt gcgcggaccc tggaccaact atttctttac cagcgtacat
      721 cagcgcgtgt acagcgacat caatatcagc gacgtcttcc gcaactttat gcaagacgct
      781 ccctccgtgc ccgggctcag ctttgccgtc acggacgatg atcgccgcga ggtgcgggag
      841 atccttgcgg aacatgggat cagcgaccgc gatttcgtgg tttgcctgca gctcggtgcc
      901 agcgactcat ccaaacgctg gcccgttgcc caattcgcgg aactggcgcg gcggctggcc
      961 gatgaaaaga atgcccggat tctcctcctc ggcgtggaat ccgaaagccc gctgggcgag
     1021 gccttcgagg cgcaggcgcc ggggctggcg gtgcaccttt tcggcaaaac caccattcca
     1081 cagctggccg ctctcctcga gtccgcacgc gcactggtca ccaacgatac gggcaccatg
     1141 cacctggcca cagccgtgcg ctgcccggtt ctgctgttgt cggtcgggta cgttcatttt
     1201 cgagagaccg gcccgtatgg cgaaggccat ctcgcaatag agcgccggaa agactacctc
     1261 gcgcctatga gcctgcagga cacggcaaca gatgatcgca atgcgatacc agccgggccg
     1321 gtgatggatt tgttcgaact gctgctgaca ccttcccacg acggctccat cccgtctttg
     1381 tccgactcgc ccgaaagggc cctgctcgac gtgtatcagt cccgctttgc cccggacggt
     1441 ttcctggaat ggtacccggt gatctgccgc cccatggtcg aagctgattt tttgcgcatt
     1501 gcataccgga acacctggct cgcccatctc tgcccacaca ttccccgcga gacccttcaa
     1561 gatggaattc agcgcctggt gtcctgtttc gcgccaactc agatggccct gctggatagc
     1621 tggtgtgagc actggggcgc agtctttctc cagctggaac aactggcccg tctggggcat
     1681 gagaagacgg agcagctccg cgcctgcctc cgccagggcc ggaacatcac aatggcccgg
     1741 gaaattgtag gcgagctcat gcggctggat gaagatacgc gcctttttgc ggagttgaat
     1801 gaggcatgcc gcccgctcat cagcatcgcg cggtttcaac gcgataacct ggaaggcgcc
     1861 gacccggcgc cgctcgcgga gatgacccag gagatctatc aggacctctt ggagcgtgcc
     1921 gcgctgatgc agctgtattt tggtgttatt cgcgacgccg tggccgccac tcacgctccg
     1981 ggttgattgt tttatcgggc cggcggtgaa agcaggcttc gcgcgtgttc gaggctgacc
     2041 tcagagacgg aaccgtcaag caggcgcgcc agttcccgca cgcgctcttc gtcggcaata
     2101 cgcgccaccg aggtgacggt gcgctttttc tgaatcattt tggccacgtg gaaatgggtc
     2161 tcggccgcag ccgcgatttg cggcagatgc gatatgcaga tggtctgatg cgaatccgca
     2221 agctcaccga gcttcgccgc caccttcttt gccacggccc cccctacccc ggcgtcaatt
     2281 tcatcgaata tcagcgtggg aatggtatcg gcgttcgcaa acacgctttt gagcgccagc
     2341 ataacccggg agatctcgcc gcctgaggca acctggcgca atggtttcaa cgcttcgccg
     2401 ggattggctg ccagcagaaa ctcgacccgg tcaatgcccg tcgggctgag ttccccgtgc
     2461 tcgaaccggg tctcaaaccg ccctcccttc atcccaagct ccgcgagtgc ggcggcaatc
     2521 tgcttatcga gcgtgcacgc ggcctttttt cgcagcacgg aaagcacccc ggcaaggtcg
     2581 cgggccttct ccagcaacgc atcgcgctgg cgctggagct gcgcgattcg ctcatcgcgc
     2641 tgctcgaacg ccgccacttc agcccgggcg cggcggcaat agtccagcac tgcgtcaatc
     2701 gatgcaccat atttacgttt caatgcgctg atcagtgtca gacgcgcatt gattccttcc
     2761 agctcttccg cgtcgaaatc gacctctgtc gtgaactcac ggagctccgc tgcgacttct
     2821 tcgatctttt cacgcgcgtc cgcaaggcgt tccacgaagg gcgcgaaacg gctgttgatg
     2881 cgggccagtt cctccacgtt gccaatagca gccgccagtg catcgacgac cgcgccctcc
     2941 tcatgttcgt acagcgaggc atatgcctgc gagctcaatt caaatatgcg ttccgcatgg
     3001 ggaaggaggt cgcggcgttc ccggcgttcc tcttcctcgc ccggttccag ggcggctttc
     3061 tcgatttctt cagcctcgtg ctgcaggaat tcaatgcgcc gggcgcgttc ccggtcgtcc
     3121 acttccagga cggcaagttc gcgttcggcg ccgcgcaggg ctgatacggc ttcggcaacc
     3181 gtgataacgt tggattcggc gccggcaaac ccgtcgagca gattcaattg gcgcgacggt
     3241 ttgagaaggg attggtgctc atgctggccg tgcagatcca ccaattcgtc gccaagcgcc
     3301 cccagcacgg ttaggggcac gggcactcca gcggcgaagg cccggctccg gccatcagca
     3361 gtaatcacac gcgaaaggat caatactccg tcgtcgagca ggatgcccga ggcatcaagc
     3421 gccgcggcca ggtcgcgggc gggatcctca atacgaaaca cggcatcgac gcgggcccga
     3481 tcggcgccgt cgcgcacccc ttcggaagac gcacgggcgc cgagcaccag attcagtgct
     3541 ccgacgatga tggacttgcc cgcgcccgtt tcaccggtca ggacattgaa accgggcccg
     3601 aattccagtt cgatctcgtc gatcagcgcg tagttctgta tatgcaggcg ttcaagcata
     3661 attccacggg ctcgtttacc gcgatcgagt acttattcta tcgccggcag cctcaaatac
     3721 gccagatatt cctggtttcc cttgggtccc ttcaatgggg atggaatcac tccacccgtt
     3781 tcgaaaccga atgcctctgc cgcggcgagc acgtcctgaa ccgtctgctc gcgcacggct
     3841 tcgtcccgga tcacgccacc cttcccgacg agcgttttcc ccgcctcaaa ctggggcttg
     3901 ataagcacca ccccctcagc cggtttccgg agcaaggtct ggagcacggg cagcaccagc
     3961 cgcagcgaaa tgaacgagca gtcggcggtg aatacctcag gaagtctcgg cagggcctcc
     4021 gcggccacgt gccggatgtt cgtgcgttcc atgacacaca cccggggatc ctgccggagc
     4081 cgccaggcca gctggccgta tcccacatcc accgcgtaga catacgcggc gccatgctgc
     4141 agaaggcaat ccgtgaaccc gcccgtcgaa gcgcccacgt cgatcgccac ccggcccgca
     4201 acgtcgaagg taaaggccgc gaaggctgct tcaagcttgt cgccgccccg gctgacgtag
     4261 cgggactcgt cgcggacttc cagcggagtg gagacatcca ggcgcatgcc gggcttgtcg
     4321 agcttccggc tgcccggacc gtatacctgt ccggtgtgaa tcaggccccg ggctttagat
     4381 cgcgacacgc cagcctggcg ttgcacgagt atgtccaacc gctccttttc catagacacc
     4441 ggccactgca gcgatcggta agtttcaggt ccgcgctgtc gccctgcccc tgtccggccc
     4501 ggtccgcccg gtcatgattt tgacccgata aggtcccgtt tgctgctcca cgccacgaac
     4561 acgccggcca gaagcaagat ggaccccaga atgatgtcca gcatgcgtgg cgtcgcctcg
     4621 tggatccacg gaaccagagc cagcatcatc accccgatga ccgccacaca aaccccgaca
     4681 atgcggaacg gcgatatgat cgtcgcgcct ccggcgtcat cgtgctcctg atcctctgcc
     4741 aatttcccga tgggcacgtc aaggcgcgcc aggaacgcct gggcccgctc cagctcatcg
     4801 ctgtgcgcgg gggcgagcca gctggtcagc tccatcatcg catacgtgat gagtgccgtg
     4861 ctcaaaaaca tggcgatctc aagtttgacc ttcaagccgc ccagaaggaa ctcctcgctt
     4921 tccggcaccc aggaaatccc ggccagcgac aggggcgact tgtgaaatga cagcgc
//
LOCUS       DAOPBQ010000251         4963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14277_length_4963_cov_4.100041, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000251 DAOPBQ010000000
VERSION     DAOPBQ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14277_length_4963_cov_4.100041"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PKW60_08925"
     CDS             complement(<1..872)
                     /locus_tag="PKW60_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPJ99593.1"
                     /translation="MAVFDYEAIAGSGKQVRGVIDADSPAAARRKLREQDLFPTSVRE
                     SSGKTGAASDIVGSSLGRIKGRDIALMTRQLAVLLEAGMSLVEALTALLEQTGNPRLK
                     KVVFDVRDRVNEGMTLADALAQHPRVFNALYANMVRAGEASGALEAVLMRLADLLERN
                     VRLRARVTSALAYPVLMACVSVGVIVFLLMVVVPKIVGTFERQNVELPGITKAMLFTC
                     NFLYAWWWAVVLAAVGALLTWRTWVKRPAGRLKWDRFKMALPYAGDLYLKVVCTRFSR
                     TLGSMLDSGLTMLNA"
     gene            complement(898..2637)
                     /gene="gspE"
                     /locus_tag="PKW60_08930"
     CDS             complement(898..2637)
                     /gene="gspE"
                     /locus_tag="PKW60_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HPJ99594.1"
                     /translation="MSLKRTARIGEILLEQGHIHQEQVDEALELQRVRPKRLGQILLD
                     LGYCEEDQVLEALSAQFGIPLDRNVTAQIDSSLTTKVPINFIREYRMVPYKRDGAVFC
                     VAVNDPVNLLPLDDLRLLLGGPVSPVLCRGSDIQGVLDSYFDRQGENAAEMIDDIVLA
                     EGETGEIHTLDSLEASERDLLDLANEAPIIKLINLLITGAIRERASDIHVEPFERDVR
                     VRYRIDGVLYEKLAVPKSQQAAVISRIKIMANMNIAEHRLPQGGRIKIRLSGKEIDIR
                     VSVIPVAFGERVVMRILEKGTFLFGLEELGLSKHDYAVFDRMIMSSHGIILVTGPTGS
                     GKSTTLYAGLQRVNSPDKNIITVEDPIEYQMPGIGQIQVRDRIGLNFAAALREILRQD
                     PDIILVGEIRDHETAEMAVQASLTGHLVFSTLHTNDSAGAITRLVNMGIEPFLVSSST
                     IAIMAQRLVRRVCPECREEYEPKPESWVELGVHADDAKGLTAWRGPGCDKCQGRGYYG
                     RIGIFELLVMSPHIQELTLKGVDSNVIKREAVREGLRTLRTDGAAKVRAGISTIEEVL
                     RVTRDDVLEGVVA"
     gene            complement(2634..>4963)
                     /gene="gspD"
                     /locus_tag="PKW60_08935"
     CDS             complement(2634..>4963)
                     /gene="gspD"
                     /locus_tag="PKW60_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HPJ99595.1"
                     /translation="PAEELEPTPVEAPPPAPTPPPRRPAVPRQTPPPASRPPVRPERA
                     GQPRQVNPDELTPSGREVPAIPVDPYRPKSSTPAGTNRGGAEEPLLTLDFEEDSLLDV
                     IKIIGAQTGKNFDIDPQMAQMKVTLISHAPIPASMAFDILGSLLQMRGYDMVEVLDGH
                     LVRIVQTGQFTEKSPLVIGRGEPKGYDTVATHIVPILYANAADLQSLLQQLGSQTAAV
                     SVYEKTNMLIITDTADGIRNMLKFIQEVDVPGYETEMEIFTLEYTRAEVLATQIQDIL
                     GVTAEGAPQPVERPARPTPVPRQPARPNVPGRPESTVVSHKETMRIVSDERLNALIVL
                     ASAPLMGQVRDLIALLDTPTDYESDNLHVYQLLNADATKVEEALNAVIGLTPRAEGQQ
                     QGAPAATAEIQPFEKKVQITSYEDRNSLLIVAAPQDYRVIRELIAQLDVPPRQVHVDA
                     VIMEVTISDNFDLSVELAGIDGEDGFGLNNVVTLASVLTEGPLAAVGNGGVLAGIIDG
                     TTELTIPDGAGGFITQKIPNIPLLLQTLEAITEVDVLAQPGLTTKDNVEAEVVVGQEV
                     PFITGSSRSLDQSAIGSSVFSRVEREDVGIKLKVKPQISEGDYVSMELEVEVSEVIQS
                     TVGADVNIVGPTLSKSNVKTETVIKDGSTGILGGLLREGTDRSIRQPPYLGDLPMVGW
                     LFRTKSNQRSKRNLVLLVSPYIIKEGIDMDRVTKYRMDQFSNANVDVLFEKGYIKRIN
                     KRRYMRNEHRPTRARVEQINAGERFGRGDARRGTE"
BASE COUNT          873 a         1697 c         1396 g          997 t
ORIGIN      
        1 gcgttcagca tcgtgagccc gctatccagc atcgagccca gcgtccgcga aaagcgcgtg
       61 cataccactt tcaggtacag atcgcccgca tagggcagcg ccattttaaa tcgatcccat
      121 ttgagacggc ctgccgggcg tttcacccag gtgcgccacg tcaggagggc gcccaccgct
      181 gccaacacca cggcccacca ccacgcgtac aggaagttgc atgtgaacag catggctttc
      241 gtaatgcccg gaagctccac gttctgccgc tcgaaggtgc cgacgatctt cggcacgact
      301 accatcagca gaaagacgat cacgcccacg ctcacgcagg ccatcagcac ggggtaggcc
      361 agggccgacg tcacccgggc gcgcagccgg acatttcgtt ccaacaggtc cgccaggcgc
      421 atcagcacgg cctcgagcgc accgctcgct tcgcccgcgc gcaccatgtt cgcataaagt
      481 gcgttaaata cccggggatg ctgcgccagg gcgtcagcca gcgtcattcc ttcattgacc
      541 cggtcccgca catcaaacac gacctttttc aaccgcgggt ttcccgtctg ttccagcaac
      601 gccgtcagtg cctcgacaag cgacatgccc gcttcgagga ggaccgccag ttgccgggtc
      661 atcagcgcga tgtcgcgccc cttgatgcgg cccagtgagc ttcccacaat atcggaggct
      721 gccccggtct taccgctcga ttcgcgcaca ctggtcggga aaaggtcctg ctcacgcagt
      781 ttgcggcgcg ccgccgcggg ggaatcggca tcgataacac cgcgcacctg cttgcccgag
      841 ccagcaattg cttcgtaatc aaatacggcc atgccggtta gccttcgcct ccgtgcttca
      901 tgcgacaacg ccttccagaa cgtcatcacg cgtcacgcgc aacacctctt caatggtcga
      961 gatccctgcg cgcaccttcg cggcgccgtc tgtccggagc gtccggaggc cttctcgcac
     1021 cgcttcacgt ttgatgacat tcgaatccac gcccttgaga gtcaactcct ggatgtgcgg
     1081 actcatcacc agcagctcaa aaatgccgat gcgcccgtag tacccccgcc cctgacattt
     1141 gtcgcagccg ggcccgcgcc aggccgtcag ccctttggcg tcatcggcgt ggacacccag
     1201 ctccacccag ctttccggtt tcggctcgta ctcttcacgg cactccgggc agacgcgccg
     1261 caccaggcgc tgggccatga tcgcaattgt tgacgagctc accaggaacg gttcgatgcc
     1321 catattgacc aggcgcgtga tcgcgcccgc actgtcgttc gtatgcaggg tgctgaacac
     1381 caggtggccc gtgagcgacg cctgcaccgc catctccgcg gtttcatggt cgcgaatctc
     1441 acccaccaga atgatgtccg gatcctggcg caggatctcg cgcagggccg ccgcaaaatt
     1501 gaggccgatc cgatcgcgca cctggatctg gccgatgccc ggcatctgat attcgatggg
     1561 atcctccacc gtaatgatgt ttttgtcggg agagttcacg cgctggagac cggcgtacag
     1621 cgtggtcgat tttcctgaac ccgtcgggcc cgttaccagg atgatcccgt gcgagctcat
     1681 gatcatccgg tcgaacacag cataatcgtg ctttgacagt cccagctctt ccagcccaaa
     1741 cagaaatgtg cccttttcca ggatacgcat caccacgcgt tcgccaaacg ccaccggaat
     1801 caccgagacg cggatgtcga tttccttgcc gctcagccgg attttgatac ggccgccctg
     1861 cgggaggcgg tgctctgcga tgttcatatt cgccataatc ttgatgcgcg agatcacggc
     1921 cgcctgctgc gacttcggca ccgccagctt ctcgtacaaa accccgtcaa tccggtaccg
     1981 gacccgcaca tcgcgctcga acggctccac atgaatatcc gaggcccgct cgcgaatcgc
     2041 gcccgtaatc agcaggttga tcagcttgat gatcggcgcc tcgtttgcca gatcgagcag
     2101 atcgcgttcc gacgcctcca gcgagtccag cgtgtggatc tctccggtct ctccctcggc
     2161 cagcacgatg tcgtcgatca tctccgccgc gttttcgccc tgccggtcaa aatacgagtc
     2221 caacacgccc tggatgtcgc ttccgcggca taaaaccggc gacaccggcc cgcccagcag
     2281 gagacgcaga tcgtccagcg gcagcaggtt caccgggtcg tttaccgcga cgcaaaacac
     2341 cgccccgtcc ctcttgtacg gcaccatgcg gtactcgcga atgaaattga tcggcacctt
     2401 cgtcgtcaac gacgagtcga tctgggccgt cacgttccga tccagcggga tgccgaattg
     2461 cgcgctcaag gcttcgagca cctggtcctc ttcacaatat cccaggtcga gcaggatctg
     2521 ccccagccgc ttcgggcgaa cccgctgcag ttccagggct tcatcgacct gctcctggtg
     2581 gatatgcccc tgttccagga ggatctcgcc aattcgggcc gtgcgtttca gactcattcc
     2641 gtgccccgtc tcgcgtcacc ccggccaaag cgttcgcccg cattgatttg ttcgacgcgc
     2701 gcccgcgtgg gccggtgttc gttacgcatg tatctccgct tgttgatgcg cttgatgtag
     2761 cctttttcaa aaagcacatc gacattcgcg ttgctgaact ggtccatccg gtacttcgtg
     2821 acccggtcca tgtcgatgcc ttccttaatg atgtacggcg acaccagcag caccaggttg
     2881 cgcttgctcc gctggttgct cttcgttcgg aacagccacc ccaccatggg cagatccccc
     2941 aggtacggcg gctgccgaat ggaacgatcc gtgccctcgc gcagcagccc gcccaggatc
     3001 ccggtcgaac cgtctttgat cacggtttcg gttttcacat tcgatttcga cagcgtcggg
     3061 cccacaatgt tcacgtcggc gcccaccgtg ctctggatca cttcggaaac ctcgacctca
     3121 agctccatcg acacataatc gccttcgctg atctgcggct tgaccttcag cttgatgccg
     3181 acgtcctcgc gctcaacccg gctgaagacg ctcgaaccga tcgccgattg atccagggac
     3241 cgcgacgaac ccgtgatgaa cggcacctcc tggcccacca ccacctcggc ctcgacgttg
     3301 tccttcgtcg tcagacccgg ctgggccagt acatctactt ccgtgatcgc ctccagcgtc
     3361 tgcagcaaca gcggtatgtt cggtatcttt tgtgtaatga acccgcccgc gccgtcggga
     3421 atcgtcagct ccgtggtgcc atcgatgatg cccgcgagta cgccaccgtt gcccaccgcc
     3481 gccagcgggc cttcggtcag caccgacgcc agggtcacca cgttgttcag tccgaaaccg
     3541 tcctcgccat caatgcctgc aagctcaacc gacaggtcga agttgtcgct gatggtcact
     3601 tccatgatca ccgcgtcgac atgcacttgc cggggcggca cgtcgagctg cgcaatcagc
     3661 tcgcggatca cccggtaatc ttgaggcgcc gcaacgatca gcagcgagtt tcgatcttca
     3721 tagctggtga tttgcacctt cttctcgaac ggctgaatct ccgccgtcgc cgcgggtgct
     3781 ccctgctgct ggccttccgc gcgcggcgtc agtccgatca ccgcattcaa tgcctcctcg
     3841 actttggtcg cgtccgcgtt cagcaattga tagacatgca ggttatcgga ctcataatcg
     3901 gtgggcgtat cgagcagcgc gatcaggtcc cgcacctgtc ccattaacgg cgcgctggcc
     3961 agcacaatca gcgcgttcag gcgctcgtcc gacacgatgc gcatcgtttc cttgtggctg
     4021 accaccgtcg attccggccg gcccggcacg ttcggacggg ccggttgccg cggcaccggc
     4081 gtcggccgcg ccggccgttc caccggctgg ggggctcctt ccgccgttac gccgagaatg
     4141 tcctggatct gcgtggccaa cacctccgcg cgcgtgtatt cgagcgtgaa aatctccatc
     4201 tccgtctcgt atcccggaac gtccacctcc tgaataaact taagcatgtt gcggatgccg
     4261 tccgcagtgt ccgtaataat gagcatgttg gttttttcgt acacgctcac ggcggccgtc
     4321 tgcgaaccga gctgctgcag gagcgattgc aggtcggcgg cgtttgcata caggatcggc
     4381 acgatgtgcg tcgccaccgt gtcatagcct ttgggttcgc cgcgcccgat gaccagcgga
     4441 cttttttccg tgaactggcc cgtctgtacg atgcgcacga ggtgcccgtc cagcacctcc
     4501 accatgtcgt agccgcgcat ctggaggagc gaaccgagaa tgtcgaacgc catcgacgcc
     4561 gggatcggcg catgtgaaat caaggtcacc ttcatctgcg ccatctgagg atcgatatcg
     4621 aaattcttcc cggtctgcgc gccgatgatt ttgatcacat ccagcaggct gtcttcctcg
     4681 aaatccagcg tcagcagcgg ctcctcggcc ccgccccggt tggtgcccgc gggcgtggat
     4741 gacttgggcc ggtagggatc gacggggata gcgggaactt cccggccgga aggcgtcagc
     4801 tcgtcaggat tcacctgccg gggctggccc gcgcgttcgg gccgcaccgg cggtcgcgat
     4861 gcgggcggcg gcgtctgccg cgggaccgcg ggccgccggg gcggcggcgt tggcgcgggc
     4921 ggcggggcct cgaccggtgt gggttccagt tcctccgcag gcg
//
LOCUS       DAOPBQ010000252         4942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14338_length_4942_cov_4.430735, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000252 DAOPBQ010000000
VERSION     DAOPBQ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14338_length_4942_cov_4.430735"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PKW60_08940"
     CDS             complement(<1..928)
                     /locus_tag="PKW60_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99596.1"
                     /translation="MAVCLLLALLAAVDCRLKHTNASIAGFHPAGASCVLFSSDFPSF
                     CAKLAGTDAATRFLSETPRPFEAFELAVRKTTGVRPTPLRWAAWLGPRFVWARWGGTH
                     GLCAHPGLLLRVVHFCRTLWGARPDKAGVYACAGHFYAWRDGFLILSSSPQYVQAALD
                     APPAGHVPIANDSSLWLVWEGGGLRIHPEADLPVEGSLNIAFQAADAPTQPAASWPSS
                     ALLTMSAPTPGDALKVWNAAIRPVKTSPVYTRIASLADSLWMQWSLGELAADWAGEVG
                     EFSVALTDVDTTETIPVPVCGVRMRAGSQEALQ"
     gene            complement(1049..1333)
                     /locus_tag="PKW60_08945"
     CDS             complement(1049..1333)
                     /locus_tag="PKW60_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99597.1"
                     /translation="MCKKMKSAYELAMERLEKTSGPAKKLNDEQRAEIAQIEKLYEAR
                     IAETRLDFDQKVSAAASVVELEELQQQLGQKISELEQERDQKKEAVWEKA"
     gene            complement(1348..1836)
                     /locus_tag="PKW60_08950"
     CDS             complement(1348..1836)
                     /locus_tag="PKW60_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99598.1"
                     /translation="MPNREELRQIYESTQVLKRPTYGIVSGYHELPYVVLGESFESGY
                     DTMRVRGTIQVSPRFLIRPSHLEPSYAEIFGEENVDAQLMGRVFGFLGFRGRPVECKS
                     EYLEVKHLRQPLDSLVAITRDEFDRYEDVTTGLIITPDTRYYPVSIERFISTVLEDEF
                     GV"
     gene            complement(1841..3271)
                     /locus_tag="PKW60_08955"
     CDS             complement(1841..3271)
                     /locus_tag="PKW60_08955"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPJ99599.1"
                     /translation="MAIPVRTWLAMLLVLPVLATCGAPKTPETPQPPSVPPAADTPPA
                     VPEPESPAPTPEPLAYQPVILEPEAAELLDQEGSGKLYQAGELLVCVLEGTHREMGFQ
                     HGRLLAGKIQHITKEGYLQRAVFDNGYTLAYAVEQSTRMARHFPPEYLDELHGILDGL
                     TAAGVSGVTYEELLIAACVAELLHHAPNSPPACSNFAVFGQWTPDGRLLHGRNLDWTI
                     GKGAQEDAVTFIYRPEGGVPFMMLAYAGVIGGVSGMNASQITIGEMTVSSPEESFDGI
                     PLQIIMRMVLEKAKTLDEAVALVQQGPRTLGWNFVLGDGKIPDARALEVDRNTVDVFT
                     PGDPHETPEMGHQALPDAVRRTNHPCGETQQRKVVQAHGAEIGLDGSDWDAVKPMAKA
                     FLAQQNTFHRYQWLGEQIQARPHTIDVSTALSLLSNGPVAANNTLHSFVFDPANQTAY
                     IANAAVAPPVTAWKTVYTRLDLSQWF"
     gene            3870..4382
                     /locus_tag="PKW60_08960"
     CDS             3870..4382
                     /locus_tag="PKW60_08960"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HPJ99600.1"
                     /translation="MYRQDEQNCHYNRRLRPLAHTLRKNMTKAEASLWKYVLCARNMK
                     GYPFRRQRPVLQYIVDFICTELKLVIEVDGTSHDTEEDQERDCRRDQELSLAGYRIFR
                     IQNEDVLQRLDEVRLRIEDVVEEIEESTPGHAGPRGHPRQRGTKKATVRRKKYNGGPG
                     RKNDTAIERT"
     gene            complement(4522..>4942)
                     /locus_tag="PKW60_08965"
     CDS             complement(4522..>4942)
                     /locus_tag="PKW60_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPJ99601.1"
                     /translation="TYIKAWLLTILRNTFINEYRRKARRPAFVELSGAEPSLDSGPDP
                     GIRYEPPDRSGTELLELLDDEVRQAVETLPTDFRQAVIMADLEDMSYKEIAEAMDCPL
                     GTVMSRLYRGRKLLRESLYDYAKELRLVRKGKNKKRS"
BASE COUNT         1037 a         1414 c         1497 g          994 t
ORIGIN      
        1 tctgcagggc ttcctgcgaa cctgcccgca tcctgacgcc acagacaggc acagggatcg
       61 tttctgtcgt atccacatct gtcaaggcca cggagaattc cccgacttcg cccgcccagt
      121 ctgcggcgag ttcccccagc gaccattgca tccagaggga atcggcgaga gacgcgatgc
      181 gggtatagac aggggaggtt ttgacggggc ggattgcggc gttccagacc ttcagggcat
      241 ccccgggcgt gggggcgctc attgtcagga gggcactcga gggccaggag gccgccggtt
      301 gcgtgggcgc gtctgccgcc tgaaaagcga tattcagaga cccttccacg gggagatcgg
      361 cttcgggatg tatgcgaagg ccgccgccct cccacacaag ccacagactg ctgtcgttgg
      421 cgatgggcac gtgcccggcc ggaggcgcgt cgagagcagc ctgcacgtat tggggagagg
      481 atgacaggat caggaatccg tcacgccacg cgtaaaagtg ccccgcacag gcgtagactc
      541 cagccttatc ggggcgagct ccccacaggg tacggcaaaa gtgcacaacg cggagaagca
      601 gccccgggtg cgcgcagagc ccatgggtgc cgccccagcg ggcccagacg aagcgtgggc
      661 ccagccaggc ggcccagcgt aacggcgtgg ggcgcacgcc ggtggttttt cgcacggcga
      721 gctcgaacgc ttcaaatggc ctcggggtct ccgacaagaa gcgggtggct gcatccgtac
      781 ccgcgagttt cgcgcagaat gaaggaaaat ccgacgagaa aagcacgcac gatgcgccgg
      841 ccggatgaaa tccggcgata gaagcattgg tgtgcttgag gcggcaatcg acagcggcca
      901 gcagcgccag gagcaggcac actgccaaca gagcgcccca actgcgcgcg gaacggcgaa
      961 cttgtggtgt ttctctcccg gcactcatcg taacgtgatc ggcacgctta acgaccggcc
     1021 ccccggacag cttgtccata cccccaactc aggccttttc ccaaacggct tcctttttct
     1081 ggtcccgttc ctgctccaat tccgaaatct tctgccccaa ctgctgctgc agttcttcca
     1141 gttcgaccac cgaagcggct gcgcttactt tttgatcgaa atcgagccgg gtctctgcga
     1201 tccgggcttc gtagagcttt tcaatctgcg cgatttccgc acgctgctcg tcgttgagct
     1261 ttttggcggg cccactcgtc ttttcgaggc gctccatcgc cagctcgtag gcgcttttca
     1321 tctttttaca catcgctgaa atcctcctca aaccccgaat tcatcctcga gtaccgtaga
     1381 gataaagcgt tcgatggaca cggggtagta ccgggtatca ggtgtgatga tcagtccggt
     1441 ggtgacgtct tcataccggt cgaattcatc gcgcgtgatc gcgaccaggc tgtccagcgg
     1501 ctggcgaagg tgtttgacct cgaggtactc ggacttgcat tccacgggac gtccacgaaa
     1561 acccaggaaa ccgaagaccc ttcccatcag ctgtgcgtcg acattttcct cgccgaagat
     1621 ctcggcgtag ctgggttcaa ggtggctggg gcgaatcaag aaacggggcg agacctggat
     1681 cgtcccgcgt acccgcatgg tgtcgtagcc ggattcaaac gattcgccca ggaccacata
     1741 cggcaattcg tggtagccgc tcacaatccc gtaggtaggt cttttgagca cctgggtgga
     1801 ttcgtaaatc tggcgtaatt cttctcgatt gggcattttt ctaaaaccac tgtgacaggt
     1861 cgagccgggt atacactgtc ttccacgccg tgacgggcgg ggccaccgca gcattggcga
     1921 tatacgccgt ctggttcgcg ggatcaaaca caaacgaatg cagcgtgttg ttcgctgcca
     1981 ccggaccgtt ggagaggagg ctgagcgcgg tcgaaacatc gatcgtgtgg ggacgggcct
     2041 gaatctgttc tccgagccac tggtaacggt ggaacgtatt ctgctgcgcg agaaacgctt
     2101 tcgccatcgg cttcaccgca tcccagtcgc ttccatccag gccgatttca gccccgtgcg
     2161 cctgaactac ttttcgctgc tgggtctcgc cgcagggatg gtttgtgcgc cggacagcgt
     2221 ccgggagggc ctggtgtccc atttcagggg tctcatgcgg gtcgccgggc gtgaacacgt
     2281 ccacggtatt tcggtccact tcgagcgcgc gggcatcggg aatcttgccg tcgccgagca
     2341 caaagttcca gcccagggtt cgcggcccct gctgtaccag tgccaccgct tcatcgaggg
     2401 tcttggcctt ctcgagcacc attcgcatga tgatctggag cgggatgccg tcgaacgatt
     2461 cttcgggcga tgacacggtc atctctccga tggtgatctg cgaagcattc atgccgctga
     2521 ccccgccgat cacgcccgca tacgcgagca tcatgaaagg aaccccgccc tccgggcggt
     2581 agatgaaggt gacggcgtct tcctgcgccc ctttgccgat ggtccagtcg aggtttcggc
     2641 cgtgcagcaa gcggccgtcg ggagtccact gcccaaagac ggcgaagttt gagcacgccg
     2701 gaggagaatt tggagcatga tgcagcagct cggctacaca ggccgcgatc agcagttctt
     2761 catacgtgac gccgctgacg cccgcggccg tcaggccgtc gagaattccg tggagttcat
     2821 cgagatattc cggggggaaa tggcgggcca tgcgggtgga ttgctcgacc gcatacgcta
     2881 acgtatatcc gttatcgaac acggcccgct gaaggtagcc ctctttcgtg atgtgctgaa
     2941 tcttgccggc caggagccgc ccatgctgaa atcccatttc ccggtgcgtg ccttccagga
     3001 cacacaccag cagctcgccg gcctggtaga gctttcccga accttcctgg tcgagcagtt
     3061 cggcagcctc gggctccagg atgacgggct ggtacgccag tggttctgga gttggcgcag
     3121 gggactcggg ctcgggtacg gcgggcggcg tatctgccgc cgggggaacg gaaggaggct
     3181 gaggagtttc cggggttttt ggggccccgc atgtggcaag cacaggcaaa acaagcagca
     3241 tggcaagcca cgttcggact gggatagcca ttgcgcacct ctacgttgtc aatctgtacc
     3301 tgatggtgaa gagtgtaccg cagaacggag ggaagattaa atcccgccac gaaacccaga
     3361 tagccgggaa atcttcatgc cgccaccagg gtgatggcat aacagttgat gcgccagtaa
     3421 ttggcgctcc attttcgggc aaaccgccac ggacgtatcc gggagtgcta gaaacaccgt
     3481 aatcactaca agggagggca gcatgaacgt tcgtatcgag gtacttgcgt tgtaagtgca
     3541 tcaaacgtac tagatggagc tgtatccacc gattcagttc gcgaaatgag agcacagcgg
     3601 gcgggcgtca ttgatggata gcgcacagat ccatttcata gggtataagc ccacaagata
     3661 aagcggatac tggaagtgat gggctgttct gaaaagcatt tgattacacg cacatcttgt
     3721 gtcccccgct ggcgggggtg gtccgcgcgc atgcgcgcgg gccgggggtg gattctcagc
     3781 ttctgatggc aaaaaatgtt ctcttttgtt tttgccgtca tttgacgcga tttgctctct
     3841 gcaatagata ttgaaataat cagaacgcta tgtacaggca agacgaacaa aattgccact
     3901 acaaccgaag gcttcgccct ctagctcata cactgcgcaa aaacatgacc aaagccgagg
     3961 caagcctgtg gaaatacgtt ttatgcgctc gcaatatgaa aggctatcct tttcgccgac
     4021 aacgcccggt cttgcaatat atcgtcgact tcatatgcac tgagctcaag ctcgtgattg
     4081 aggtggacgg gacctctcat gataccgagg aggatcagga aagagactgc cgacgagacc
     4141 aagaactctc tctggcagga taccgcatct tccgaataca gaatgaggac gtcctgcagc
     4201 gcctggacga ggtacgctta cgcattgagg atgtagttga ggaaatagaa gaatccaccc
     4261 ccggccacgc gggcccgcgt ggccaccccc gccagcgggg gacaaagaaa gcgaccgtcc
     4321 gcaggaagaa atataatgga gggccaggac ggaaaaatga cactgcaatc gagcggacat
     4381 aagatttcga aggtacgtca agtaagaccg tagtgtgtta agaacggcat ccaagggcca
     4441 aggagcggag gatgaatgtt tcgcgacggc gcttgccgct tcttcggcct cttcgacaaa
     4501 aagagcgggt ggtttgagcg cttacgagcg ctttttgttt ttccctttgc ggacgaggcg
     4561 taactctttg gcgtagtcat acaggctttc acgcaagagc ttgcggccgc ggtacaaacg
     4621 cgacatgacc gtgccgaggg ggcaatccat cgcctccgca atttccttgt atgacatatc
     4681 ttccagatca gccatgatca cggcctggcg gaaatcggtg ggaagggtct ccacggcctg
     4741 ccgcacctcg tcatccagga gttcgagaag ctccgtcccc gagcgatccg gcggctcgta
     4801 gcggatgccg gggtccggtc ctgaatccag cgaaggctcc gcgccggaca attcgacgaa
     4861 ggcgggccgg cgcgccttgc gccggtattc gttgatgaag gtattgcgga gaatggtcag
     4921 gagccacgct tttatatagg tg
//
LOCUS       DAOPBQ010000253         4934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14368_length_4934_cov_4.917606, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000253 DAOPBQ010000000
VERSION     DAOPBQ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14368_length_4934_cov_4.917606"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="PKW60_08970"
     CDS             complement(<1..1310)
                     /locus_tag="PKW60_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99602.1"
                     /translation="MADDKPKKNNGSEEDAGGGRFESDSGLGNLPPLSDFESAADTSG
                     TNLPPLDASESGGDMREDSGVPSALPPISDIPVETPVHTPKSGPPDFESPSAFDTPMS
                     ALDASLDSPQPETAFQDLAADSDFTPETPELGPGPDSEMETPLFDSAFGTPETPHGIA
                     GTGAPTRAMQTPMFGGDDDLGFDEGAFTPGSDRGFASSTPAPDFGTESPETAGVAAPP
                     PQFAPQPQMPLPMAAAPAKGGGMNTMAAVLLALVVGAIGVAAGYFLWPTLQPRLGLST
                     NPLQEQYDRWENEVPGLQQKASQLAEIQTQLDQIGGIKDPNELVAEIKRLTDEKAALD
                     KQVSDVQLEAQSAQEQLAKVRQDLAVTEKNLDEKLDAYAEADADYEELLNQISIVRAR
                     DNGLRAEVERLEELVGRLEDSNARRVATKDALSHATERLAVTLK"
     gene            complement(1691..3379)
                     /locus_tag="PKW60_08975"
     CDS             complement(1691..3379)
                     /locus_tag="PKW60_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ99603.1"
                     /translation="MKSSADDVQFRWFSAATLILLGFAAYINSFSGGFVWDDASSILL
                     HKHVQDPSKIGQLFREDLHAFGRGQGNFYRPMLAISFMADYSRAFDGRMEGGVPQPAV
                     FPFHLTNTILHTGAALWLFVLLVRAGAPRFVCWAAPALWIAHPLHTEAVAYISGRGDS
                     MAAFFMFAALWFGLWETSGRTRAVGVSLSAVFFLTAILSKESALVLPFLFVPLLLWMP
                     QFDGTPACRSTRLGRLPALGAMTALLAVYAGLRATTLNFGSDSTVPEQSAVERLLQTG
                     QALAVYIKLIFVPIGLHMERTLDGIPAWAAFAGFAVLLGILAVVVFAVRKGQPRIAMA
                     GAWFLITWFPISGLIALNAPLAEHWLYVPLAGILWMLLEFVWLACGRRPVPWPAIFAV
                     CVCCLLLTALTVHRNRDWRSNEALYLATLKENPRSIRVQYNLAVTYQDLLNNPAGARR
                     HFERVVALYREKKTSEGLSQSLFWDDELEAHLSLGDILSPVEPGKAMEHYAQVLSVSP
                     DNGREGLVGMAAFGMGRCSLQLGQFEQAARFLKKAAELLPELKGQIAEMMPGSA"
     gene            complement(3466..4188)
                     /locus_tag="PKW60_08980"
     CDS             complement(3466..4188)
                     /locus_tag="PKW60_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99604.1"
                     /translation="MRKSNALRLAALIVCVALATGCSEKKSAAPPPPEESTLPIHPNA
                     QEIADEIAVGLAPLNDVLSLEAGITPELRAQLIEYLEMAKHTHGTTEWGQKGLVLAAN
                     RLEDLLEEAKASMLTDVALFLCDLIETLEPDNTKVPRFREWARIQLERPLVKIVGWFE
                     FEEPRFGSDEIYVLLEVYVPATGKTQRPRVMEGEEFMNLKFHRIIGKKRGVVLEYLPT
                     KDRFAVYGPGRRAAMKNADPNF"
     gene            complement(4328..>4934)
                     /locus_tag="PKW60_08985"
     CDS             complement(4328..>4934)
                     /locus_tag="PKW60_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99605.1"
                     /translation="YQVLKSMLEGNYAEAMRRYSQMEAQSSGPGMPSPTGMQQQLPAP
                     IDLKESIKADLQNAKDTHSNTDYGQKGLQRVGNLFERDIVAAYDRAQENPQMYDVLFM
                     AGDLLQVLEPESSMLARYRQLAKDQINRPHVKVTGNTTMDGVTIWFLQVYLPETHTTQ
                     SLQVRQGDEFLGLRFVGTLGNNRAIELEYLKLNERFVVPAP"
BASE COUNT          985 a         1468 c         1472 g         1009 t
ORIGIN      
        1 tccttcaagg tgaccgcgag gcgctcggtg gcgtgggaca acgcgtcttt cgtggccacg
       61 cgccgggcgt tggaatcttc aagccgccct accagttctt ccagacgttc gacttccgcc
      121 cgcaacccat tgtcacgggc ccgaacgatg gaaatctggt tgagcagttc ctcgtaatct
      181 gcgtcggcct cggcataggc gtcaagtttt tcgtccaggt ttttctctgt gacagccagg
      241 tcctgccgca ccttggccag ttgttcctgc gcggattgtg cctccagctg aacatcggag
      301 acctgcttat ccagcgcggc tttttcgtcc gtcagccgct tgatctcggc cacgagttcg
      361 ttggggtctt taatcccgcc gatctggtcg agctgcgtct ggatttcggc cagctggctg
      421 gctttttgct gcaggccggg cacttcattc tcccaccggt cgtactgttc ctgcagcggg
      481 ttggtgctga gaccaagccg cggctggagc gtgggccaca ggaaatagcc tgccgccacg
      541 ccgatcgcgc caaccaccag cgcgagcagg accgcggcca tcgtgttcat gcctccgccc
      601 ttggcaggcg cggccgccat gggtagcggc atctgcggct ggggtgcaaa ttgcggcggc
      661 ggtgcggcca ctcctgcagt ctcgggagat tcggtgccaa aatcaggcgc cggggtggac
      721 gaggcgaaac cacggtcgga cccgggagta aaggcccctt catcaaagcc caaatcgtca
      781 tccccgccaa acatcggggt ctgcatggcc cgtgtgggtg ctcctgtgcc ggcgatgccg
      841 tgaggcgttt cgggcgtgcc gaacgcgctg tcaaacagag gcgtttccat ctccgaatcc
      901 ggcccggggc caagctcggg cgtctccggg gtgaaatcgc tgtccgcggc aagatcctga
      961 aacgctgtct ctggttgcgg cgaatcgagc gacgcatcca aagcagacat gggcgtgtcg
     1021 aacgcactgg gcgattcaaa atccggcgga ccagacttcg gggtatggac gggagtctcg
     1081 acggggatgt cactgatggg cggcagcgca ctgggcacgc cggaatcttc ccgcatgtcg
     1141 cctcccgact cgctcgcatc caagggaggc aggttcgtgc ccgatgtgtc cgccgccgat
     1201 tcgaaatcac tcaacggagg gagattcccc aggccgctat cactctcgaa ccggccgcct
     1261 ccagcgtctt cttcactgcc gttgtttttt ttcggcttgt catcagccat tggaaaaacc
     1321 tccgttagac gacgttgcac ttcaagacca caaagtcctt cagtttcacc attctagcac
     1381 gggttcccgc acgtgtcaac aaatcccctc ggcagattca gccactcaca caaccggcta
     1441 cataccctac ttttaggctc gcaaggagat tatagtcttt tcccgggttt gtccgcacga
     1501 cacccaaaaa cgccccaaaa tgataaattc aagtattctg caatttggta tgctgtgccc
     1561 ggaagtctgg cggtttgcag tgctttgtga ctccccctca cgttccccga tgtccccatg
     1621 ggtacgattg taaacgaaag cagtgatacc tgcaagtgct tgttttttct cgaagtgcag
     1681 gcacgagcac ttaagcgctc cccggcatca tttccgcaat ctgccctttc aactccggta
     1741 gaagttctgc tgctttcttc aagaagcggg cggcctgctc gaactgcccg agttgcaaac
     1801 tgcagcgtcc catgccaaag gcggccatgc ccacgagtcc ttccctgccg ttatccgggg
     1861 agacagacag cacctgggcg taatgttcca tggctttacc cggttccact ggagacagga
     1921 tatcccccag ggacagatgg gcttccaatt cgtcgtccca gaaaagagac tggctgagcc
     1981 cttcactcgt cttcttctca cgatacaatg caaccacgcg ctcgaagtgt ctgcgcgctc
     2041 cagcggggtt gttgaggagg tcctggtagg tcacggccag attgtattga acacgaatgg
     2101 accgagggtt ttctttgagc gttgcgaggt aaagggcttc gttgctccgc cagtcgcggt
     2161 ttcggtggac ggtcaaggcc gtcaacagca ggcaacatac gcatactgcg aaaatcgcgg
     2221 gccagggtac agggcgccgg ccacatgcca gccacacgaa ttcgagcagc atccagagta
     2281 tgccggcgag gggaacgtag agccagtgct ctgccagggg cgcgttcaag gcaatcaacc
     2341 cggagatggg gaaccacgtg atcaaaaacc aggcgccggc catggcgatg cgcggctgcc
     2401 ccttccgcac cgcgaacact accacggcga ggatgccgag aagcacggcg aaccccgcaa
     2461 aagcggccca ggccgggatg ccgtccagcg tccgttccat atgcaagccg atgggcacaa
     2521 agatgagctt aatgtagacg gccagcgcct ggccggtctg gagcaggcgc tcaaccgcgc
     2581 tttgttcggg caccgtggaa tcggagccga agttgagggt ggtcgcgcgc agcccggcgt
     2641 agacggcgag cagggctgtc atggcgccca gcgcgggcag gcggcccagg cgcgtgcttc
     2701 ggcaggccgg ggttccgtcg aattgcggca tccagagcaa cagcggaacg aacagaaacg
     2761 gaagaacaag cgcggattcc ttgctgagga tggcggtgag gaagaataca gcgctcaaac
     2821 tgactccaac ggcgcgtgtc cggccgctcg tttcccacaa gccaaaccaa agtgccgcaa
     2881 acatgaagaa cgcggccatc gaatcccccc ggccgctgat ataggcgact gcctcggtgt
     2941 ggagcggatg ggctatccag agcgcaggtg ctgcccaaca cacgaacctc ggtgcgcccg
     3001 cccggacgag caggacaaac agccacagcg ccgcacctgt gtgaaggatg gtattggtga
     3061 ggtggaaagg aaacacggcg ggttgcggaa cgccgccttc catgcggccg tcgaaggctc
     3121 tggagtaatc cgccatgaag gagatcgcca gcatgggccg gtagaaattg ccctgacccc
     3181 ggccgaaagc atgcaaatcc tcgcgaaaca actggccgat ttttgatgga tcctgcacat
     3241 gtttgtgcag aagaatggag gaagcatcat cccagacgaa cccgcccgaa aacgagttga
     3301 tgtaggcggc aaatccaagc aggatgagcg ttgccgcgct aaaccagcga aactgcacgt
     3361 cgtcagcaga tgacttcatc gcggcgcgat gtctccgagg ttcagtggtt tatatgccgg
     3421 gactatagca aattgcgcgg cggcttgtct ttatccgcga gcaactcaga agttggggtc
     3481 ggcatttttc atcgcagccc ggcgtccggg accgtacacg gcgaaacggt ccttggtcgg
     3541 aagatattcg agaacgacgc cccgtttttt cccgatgatg cgatgaaact tgaggttcat
     3601 gaattcctcg ccttccatca ccctcggacg ctgggtcttg ccggtcgcgg ggacgtagac
     3661 ctcgagtaag acgtaaattt cgtcgctgcc gaaacgcggt tcttcgaact cgaaccaccc
     3721 gacgatcttc accagcggcc gctcgagctg tatccgggcc cattcgcgaa agcggggcac
     3781 cttggtattg tccggttcaa gagtttcaat gagatcgcac aagaaaaggg ccacgtccgt
     3841 gagcatactg gccttggctt cttcgagtaa gtcttcaagc cggttcgccg ccaaaacaag
     3901 gcccttctgg ccccattcgg tggtgccgtg agtatgcttg gccatttcaa gatactcgat
     3961 gagctgagcc cgcaattcag gggtaatgcc ggcttcgagc gagaggacgt cattgagggg
     4021 cgccagtccc acagcaattt catcggcaat ctcttgtgcg ttggggtgta tgggcagcgt
     4081 agattcttca ggcggaggcg gggcggcgct cttcttttcg gaacagcccg tcgccagcgc
     4141 gacgcataca ataagggccg ccaaccggag tgcgttgctt tttctcatgt agccgacctc
     4201 gtagcgcgct ctggtaacca aaaccgcggc ccgtcttcag cttcgcgggc cgcggcgagt
     4261 ctgcactcat tcgtaagggc aggcgtgcgc caggagcacg cgtctgccat ccggcaatgc
     4321 cctggtatca gggcgccggg acaacaaagc gttcgttcag cttcaagtat tccagctcaa
     4381 tggcgcggtt gttgccgagc gtgccaacga aacgtaaacc caggaattcg tcaccctggc
     4441 gcacctgcag gctctgggtg gtatgtgttt cgggcaggta tacctgaaga aaccagatgg
     4501 tcacgccatc catcgtggta ttgccagtca ctttcacgtg cggccggttg atctgatctt
     4561 tggccagctg gcgataccgg gcgagcatgc tgctttcggg ttcgagcacc tgcagcaaat
     4621 cgcccgccat aaagagcacg tcgtacatct gggggttttc ttgcgcccgg tcgtacgcgg
     4681 cgacgatgtc acgctcgaag aggttgccca cgcgttgcag ccccttttgc ccatagtccg
     4741 tattggaatg ggtgtccttg gcgttctgga gatccgcctt gatgctttcc ttaaggtcaa
     4801 tcggcgcggg cagctgttgc tgcatgccgg tgggagacgg catgcccggg cctgaagact
     4861 gggcttccat ttgactgtag cgccgcattg cttcggcata attcccttcc aacatgcttt
     4921 tcagcacctg atat
//
LOCUS       DAOPBQ010000254         4879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14556_length_4879_cov_4.152156, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000254 DAOPBQ010000000
VERSION     DAOPBQ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14556_length_4879_cov_4.152156"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKW60_08990"
     CDS             <1..327
                     /locus_tag="PKW60_08990"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HPJ99606.1"
                     /translation="ERAGATVVSQVRYKFGQDSPPGFAAVVMLDESHCSAHSYADLGL
                     IAMDMFTCGTTDPRDVLFYLREEVDLGDVEVREFGRFAVDQDAEDGAMLPSLDREGIG
                     VLTRTA"
     gene            305..1396
                     /locus_tag="PKW60_08995"
     CDS             305..1396
                     /locus_tag="PKW60_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HPJ99607.1"
                     /translation="MSSRELHDGRQDGLTPLESLRLDSVESFSGLVRGMGKTAFGGRQ
                     LGLAFDTLLTMARDEECRVVLTLSGAMTVAKQGSIICEMVDRGLVDVIVATGALMAHG
                     LTESIGLTHYAVSPDADDLELFEKGYNRIYDTLEMETNLNSVAELVADVLAAEDPPDG
                     MWSSASFCRAVGRRLDQQDEGPGILRSAYQRGVPVFIPAFTDSELGLDFATWAMKAAM
                     KKLGIREGDQADPQAVFTALPPFNPFLDLQHYARFAGSAKRLGIFTIGGGVPRNWSQQ
                     VAPFYDITADRLNIRLPEVRFQYGVRICPEPVHWGGLSGCTYSEGVSWGKFVAPEDGG
                     KYAEVPADATLVWPLLMKAVFEALDHDSE"
     gene            1402..2295
                     /gene="speB"
                     /locus_tag="PKW60_09000"
     CDS             1402..2295
                     /gene="speB"
                     /locus_tag="PKW60_09000"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPJ99608.1"
                     /translation="MRRELPISQNFLALERSQSALQSSPVVIQQAPFERTTSYGKGAA
                     KGPQAILKASHQVELFDAALDLELVAAIPGIATLPPIAPGKRDGRQYARVLRDETAHW
                     IKEGKFVVTLGGEHTAVVGAIQAHCEAWDELTVLQLDAHSDLRDEYDGSPWNHACAMT
                     RVLDFHSHIVQVGIRSQCREERDKTDALRTPVIYAHEIPGAPPGEDAWIKRVIGACRK
                     RVYVTFDCDVLDPAVMPATGTPEPGGLNWQHLDALFRRLCKVREVVGLDISELAPISG
                     LHHPQFTMAKLLYRFLGIRFQ"
     gene            2375..2578
                     /locus_tag="PKW60_09005"
     CDS             2375..2578
                     /locus_tag="PKW60_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99609.1"
                     /translation="MPPANDFPDDYGWDDESMQRRERRRKTKDKRRYERIDRDPETEK
                     PRKSSERHSRNNREWADDTLDED"
     gene            complement(2654..2887)
                     /locus_tag="PKW60_09010"
     CDS             complement(2654..2887)
                     /locus_tag="PKW60_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99610.1"
                     /translation="MSAYTVRLSPDAARVWELNPTKRNGASWRSCQLATAMLQARVHG
                     CHIFFIVSPEGDLLAQGDTGMTQGKDSRFVQKT"
     gene            3468..3959
                     /gene="folK"
                     /locus_tag="PKW60_09015"
     CDS             3468..3959
                     /gene="folK"
                     /locus_tag="PKW60_09015"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPJ99611.1"
                     /translation="MAVQLSLGSNLGVRRENLEAALARLGQLPETSVTRRSRVYETEP
                     YGVTGQPGFLNMAAEVETELQPLELLDAIKTIERQLGRQHTERWGPRVIDIDIVLWDD
                     LVLETSCLTIPHPDFRKRAFVLIPMAEIAPQALDPLTGMTVAQLAAQPNLHGRIRNPE
                     SGD"
     gene            4029..4826
                     /gene="panB"
                     /locus_tag="PKW60_09020"
     CDS             4029..4826
                     /gene="panB"
                     /locus_tag="PKW60_09020"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPJ99612.1"
                     /translation="MPRIRTTTFRKRKEAGEKITVLTAYDYPSAKLLDECGIDAILVG
                     DSLGTTVQGRDDTLGVTMDQMIYHTSLVSRAAGHAMVIGDMPFLSYQLDAEDAVRNAG
                     RFVTEGGAQAVKLEGSVDKFGHSIAAIVKTGIPVMAHIGLTPQSVHQLGGYKVQGRGD
                     QARQQLKEEALGLQQAGCFAIVLELVQQDTAAEITEMLSIPTIGIGAGPHCDGQVLVM
                     HDMLGFGPQRSFCKVFGDVRGVMETAFQNYIREVREGAFPTEEHGHR"
BASE COUNT          991 a         1462 c         1468 g          958 t
ORIGIN      
        1 gaacgtgcgg gcgcgactgt cgtttcacaa gtccggtaca aatttggcca ggactccccg
       61 cccgggtttg ccgcagtggt catgttggat gagagccatt gctccgccca ctcctatgcg
      121 gacctggggc tgatcgccat ggatatgttc acttgcggaa cgaccgatcc gcgcgacgtc
      181 ctgttctatc tccgggagga ggttgatctg ggcgacgtcg aagtgcggga attcggccga
      241 tttgccgtgg accaagacgc cgaggatggc gccatgttac cgtctttgga tcgagaggga
      301 atcggtgtcc tcacgcgaac tgcatgatgg caggcaagac ggtttaaccc cgctcgaaag
      361 cctgcggctc gattcggtcg agtcgttctc cggcttggta cggggcatgg gcaaaacggc
      421 gttcggagga cgacagctcg gtcttgcctt cgataccctt ctgacgatgg cccgggatga
      481 ggagtgccgt gtcgttttga cgctgtccgg cgccatgacg gtcgccaaac agggttccat
      541 catctgtgag atggtggacc gcggtctggt ggacgtcatt gtggcgacgg gtgcgctgat
      601 ggcccacggc cttactgaat ccatcggcct gactcactac gctgttagcc cggacgcgga
      661 cgacttggag ttgttcgaaa aagggtacaa ccgcatttac gacacgttgg agatggaaac
      721 gaacctcaac tcggtggccg agttggtggc ggatgtactg gcggcggagg acccacccga
      781 cgggatgtgg tcctccgcct ctttttgccg ggcagttggc cgccgcctcg accagcagga
      841 tgagggcccg ggtatcttgc gcagcgccta ccagcgcggc gtgcccgtgt tcatccctgc
      901 gttcaccgat agtgaactcg gcctggactt tgccacgtgg gccatgaaag cggccatgaa
      961 aaaactgggc attagggagg gcgaccaggc tgatcctcag gccgtgttca cggccctgcc
     1021 gcctttcaat ccgttcctcg atttgcagca ctacgcccgg ttcgcgggca gcgccaaacg
     1081 tctgggtatt ttcaccattg gcggcggtgt gccccgcaac tggtcgcagc aggtcgcccc
     1141 gttctacgac atcaccgccg accgcctgaa catccggctg ccggaagtgc gtttccagta
     1201 cggcgtgcgc atttgcccgg aaccggtgca ctggggcggg ctttcgggct gcacgtattc
     1261 agagggagtg agctggggca agttcgtggc gcctgaagac ggcggcaagt acgcggaagt
     1321 tccggccgat gccaccctcg tgtggccgtt gttgatgaag gccgtgtttg aggcgctgga
     1381 tcacgattca gaataagaac catgcggcgc gagttaccta tctcacagaa tttccttgcc
     1441 ctggaacgga gccagtctgc cctgcaatct tcgccggtcg tgattcaaca ggcgccgttt
     1501 gagcgcacta cgtcgtacgg caaaggcgcc gcaaaaggcc cgcaggccat tctcaaagcg
     1561 tcgcatcagg tggaactctt cgacgccgcc ctcgacctcg agctggtcgc tgccattccc
     1621 ggcattgcca cgctgccgcc cattgcgccc ggcaaacggg atgggcgcca atatgcccgg
     1681 gtgctccgcg acgaaaccgc gcactggatc aaagaaggca agtttgtcgt cacgctggga
     1741 ggcgaacata ccgcggtggt cggtgcgata caggcccatt gcgaagcctg ggacgagctg
     1801 accgtccttc agctcgatgc ccacagcgat ctccgcgatg aatatgacgg ctcaccctgg
     1861 aatcatgcct gcgccatgac ccgcgtgctc gatttccaca gccacatcgt tcaggtgggc
     1921 atccgaagcc aatgccggga agaacgggat aaaacggatg ctctgcgtac ccctgtcatt
     1981 tatgcgcatg agatccccgg ggctccgccc ggcgaggacg cctggatcaa gcgggtgatc
     2041 ggcgcctgca ggaaacgcgt ctatgtgacc ttcgattgcg atgtccttga cccggcagta
     2101 atgcccgcca cgggtactcc agaaccggga ggattgaact ggcagcatct ggatgcgctg
     2161 ttccggcgtt tgtgcaaggt gcgcgaagtt gtggggctgg acatttctga actggccccc
     2221 atctccggac tgcatcatcc gcagttcacc atggccaaac tcctgtaccg gttcctggga
     2281 attcgcttcc aataacctcg cttatgtgtg agatacccca cttctgaagg aaaactgatt
     2341 ccgatgtacg gcgctgggaa aaaggagaag ggaaatgcct cccgcgaatg atttcccgga
     2401 tgattacggt tgggacgatg aatccatgca gcggcgtgaa cgccggcgaa aaacgaagga
     2461 taaacggcgc tacgagcgca tcgacaggga tcccgagacc gaaaaaccgc gaaagagttc
     2521 ggaacggcat tcccgtaata accgcgaatg ggccgacgac accctggatg aggattagtc
     2581 gcggcagatg gaaccttccc gcgggtttgg gcacgccttt ccccgtccag acgccggggc
     2641 tgacgcggca tggtcacgtc ttctggacga accgggaatc cttgccctgg gtcatgccgg
     2701 tatccccctg ggcaagcaga tcaccctcgg gcgatacaat gaaaaaaatg tggcatccgt
     2761 gaacccgggc ctgaagcatc gctgtcgcca gttgacagct tcgccaggac gcaccgttgc
     2821 gtttggtggg gtttagctcc cacactcttg ccgcgtccgg cgacaaccgc accgtgtacg
     2881 cgctcatgac ccctctccct ctcgacacgc agccgggccg actctcgaca aacatcttcc
     2941 cagatgagca accgcagctt ttctcaagtc aaaatactaa cagaaacaca tcgctatatc
     3001 aagactcttt tgtgaaaaca tctcgactgc acatcgcaaa atgcgtgccc tgattgcggt
     3061 ttcgcctggc cagtcgcgca gaacagaggc cgttatcgtt gaaaatcttt caaaacctat
     3121 gtcctgcagt cagatggaga ctgcactctc ccggacagaa tgaatggggg ggtaaggccg
     3181 ctccctgggc ttatgtaatg cgattgccgc gtagtgtgta aggcgtttcc cgatgcgaga
     3241 agatgcaggg aatgacagag cctgtctcga tgccaggaaa cggtgccctt tcagagtctc
     3301 ctgacaacat ccgtgctcgt aatcgccgag attagtgcat ctgacaaaac tcgccactag
     3361 gcagatttac cttggtttgc actcaggctc tgtggtatcc tacgctggtt gccggtggcg
     3421 ggaggaggcc atggtgcccc agtgacagag gcagtaaggc gcgtagcatg gccgtacagt
     3481 tgagtctcgg tagcaatctg ggggtacgtc gagagaatct tgaagctgcc ctggccaggc
     3541 tggggcagct tcccgagact tccgtaacgc ggcgttcccg cgtttacgag acggaaccgt
     3601 acggagtgac cgggcagccg ggcttcctca acatggccgc ggaagtcgag acggaactgc
     3661 agccgcttga acttcttgac gccattaaaa ccatcgaacg ccaattgggc aggcagcata
     3721 cggaacgctg gggtccccgc gttatcgata tcgatatcgt gttgtgggat gacctggtgt
     3781 tagaaaccag ctgcctgacc attccccatc cggattttcg gaaacgggcg ttcgtgttga
     3841 tccccatggc ggagattgcg cctcaggcgc tcgatcccct caccggcatg acggttgccc
     3901 agcttgctgc gcaacccaac ctgcatggcc ggatccgcaa cccggaatcc ggcgactagg
     3961 ccacgaaccg ctccgcttgg agccctgaag ggaccgggac ggcgacgtca cggttcggag
     4021 gatctaggat gcctcgaatc cgtaccacga cctttcgtaa gcgcaaggag gcgggggaga
     4081 agatcaccgt cctcacggcc tacgactacc cttccgcgaa attgctcgac gaatgcggca
     4141 tcgacgccat tctcgtaggc gattcactcg gcacgaccgt ccagggcagg gacgataccc
     4201 ttggcgtgac catggaccag atgatctacc acacgtctct ggtatcccgg gccgcagggc
     4261 acgccatggt catcggcgac atgccctttt tgtcgtacca gctcgatgct gaagacgccg
     4321 tccgaaatgc cggccgcttc gtgacggaag gcggcgcgca ggccgtcaaa ctcgaaggct
     4381 ctgtggacaa gttcgggcat agcatcgccg caatcgtcaa aaccggcatt cccgtcatgg
     4441 cccacattgg tctcacccct caatcggtcc accaactcgg cggatacaag gtccaggggc
     4501 ggggcgacca ggcccggcaa caactcaagg aagaagcgct cggcctccag caggccggct
     4561 gtttcgccat cgtccttgag ctggtccagc aggataccgc ggccgaaatc accgaaatgc
     4621 tcagcatccc gaccatcggc attggcgccg gcccccactg cgacgggcag gtcctcgtca
     4681 tgcacgatat gctcgggttc ggcccccagc gcagcttttg caaggttttt ggcgatgtgc
     4741 gcggcgtcat ggaaacggcg ttccaaaact acatccgcga agtccgcgaa ggcgcgtttc
     4801 ccacagagga acacggccac cgatgaacat ttttcataca gctgacgcca tgcgcgcctg
     4861 gtcggaagca cagcgcgcc
//
LOCUS       DAOPBQ010000255         4872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14586_length_4872_cov_3.100270, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000255 DAOPBQ010000000
VERSION     DAOPBQ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14586_length_4872_cov_3.100270"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKW60_09025"
     CDS             complement(<1..277)
                     /locus_tag="PKW60_09025"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPJ99613.1"
                     /translation="MRNKTLQKGLLILEALAEEARDFSLAEVAAMSALGKSQACRLLQ
                     TLAAMGYVVRDPRTRRYRIGLRTLELSASVLSRMELHRMGITYLHQLS"
     gene            934..3651
                     /locus_tag="PKW60_09030"
     CDS             934..3651
                     /locus_tag="PKW60_09030"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPJ99614.1"
                     /translation="MNGLCVAVSAAVLVLLIFGLSVPAVAGEGVDVMTAGRLRCEYLN
                     NPLGIDANPPRLSWIVESGERGAKQTAYRILVASTREALASDQGDLWDSGKVDSADSV
                     LISYAGQALGSRQHCYWKVKVWDAHGAESAWSQPAEWSMGLLEPADWQGGYIGLDAAK
                     GDPRHPWLRKSFELDSAPTAARVYVNPLGYYELHVNGELVDDSPLTPAMTQFNKRSFY
                     LTHDVTGYLRPGINSIALWTGCGWYREGLPGVACAGPLVRAQLEAELPSGAQITIATD
                     STWKAHPSPLTTIDVRHPGHYGGERYDANANIPAWASIGFDASEWSDAVAVEPVPHTV
                     SAQPVEKNAVLRRYDPARIRFLRENEWLIDFDTNLAGLFEIQFTGLAPGQVVKMEYAD
                     FLPAEGFDPFGQVDEYVAVGGGRETFRNRFNYHAFRYVRITGIDVATQTAGIAAYLVR
                     TDFPVYGSFACSNPVLTRVYDMVHYTLECLSLGGYIVDCPHIERLGYGGDGQASTPTA
                     FTLFGMGPLYTAWLASWRDCQRPDGGMPHTAPNPFRAGGGPYWCGFIIAASWEMYQDC
                     GDVRILDVNYPAMQQWLGYVETYTKEGLLRPWPETDYRSWYLGDWARPDRREKQAERS
                     VDHVNNCFVVQCYDWMANIATVLGRAEDAAKYREEAAARRKRVHEAFYDAARGTYVDD
                     TQLDLAYPLLAGVVPDELRDAIAKRLEEKIRVEHKGHMDVGLAGVPLLTDALLQMDRN
                     DLIFGYISTETYPGWGYMLAQGATTTWEHWDAERSHIHNCYNGIGAWFYRGLAGIRPD
                     ASAPGYAHFLLKPSVTGDLTWVSARQDTARGVIESVWRIEDGQFVWDVRIPANTTATV
                     MVPTSDPGAVAESGTPVSEADGLVQVRSEDGRAVFEAAAGRYRFTAPLQ"
     gene            complement(3680..4669)
                     /locus_tag="PKW60_09035"
     CDS             complement(3680..4669)
                     /locus_tag="PKW60_09035"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPJ99615.1"
                     /translation="METVAGNPAPESQPVFATRGVVITPADLTLNWPERAKRARLTTI
                     ALHPFPRNVIEFVRTRDGEMFLDTCRELGLQVEFELHAMADLLPRTHFDTRPEFFRMN
                     DDGERTPDANLCVHSRGALDLESVGGHEVKGARILKPSTGRYYFWGDDGLPWCQCPKC
                     REFPDADQALLLENFLAQALAAFDERAQLAHLAYVNTLTPPSIVKPDPRVFLEFAPIR
                     RKFDVPLNDPDCAENKEHLELLDANLKVFPAETAQVLEYWLDCSLFSNWREPHVKLPW
                     SEAVLQADLDEYGARGIRHITTFAVYLDAHYLQTYGEPPIASYGQALADWRPR"
BASE COUNT          901 a         1379 c         1709 g          883 t
ORIGIN      
        1 cggacaactg gtgcaggtag gtaatcccca tgcggtggag ctccatgcgg gacaggaccg
       61 aagcggacaa ttcgagggtg cgcagtccga tccggtacct gcgggtgcgc ggatcacgga
      121 cgacgtagcc catggcggca agggtctgga gaaggcggca ggcctggctc ttgcccaggg
      181 ccgacatcgc ggcaacctca gccagggaga aatcgcgggc ttcctcggcc agcgcctcca
      241 ggatcaacag gcctttttga agcgttttgt ttcgcatatt gcaactacac agcagtgtat
      301 tgccaaatag gcaataagtc aatgccaaaa tccgagccag cgccgtttct gtccggtgta
      361 ggggcggccc cctgtggccg cccatccgga taaccccacg cgcgcgtcca tcctcatgcg
      421 gtggttttga tccaaggcag gcgccgcccg tcctgcgtct tgtgtccccc gctggcgggg
      481 gtggccgcga agcggccggg ggtggacgag cgcaccgcgt ggtcgtcttg aagaagggca
      541 gacacagggg tctgccccta cgcgctgcga aaacaggaat ggacctggga agccttaggc
      601 ggccgcccag tgccatgcat ccatgcgtcc ggtgtagggg cggccccccg tggccgccca
      661 tccggatcgc cccacgcggc tgcccatctt cacgcgttca ccttgataca ggtcaagtaa
      721 tgccggcacc gcgtcttgtg tcccccgctg gcgggggtgg ccgcgaagcg gccgggggtg
      781 gaccgattca gggcaggcac gggggcctgc tcctacggag gataatgaag cctcgtgcgg
      841 agtttctggc cggctgcgtt tgcataccag ggcgcagaac gcggatactc aaggggtccg
      901 gtgccttggg agacaaagac acgaggtgaa gaaatgaatg gactgtgcgt ggccgtcagt
      961 gcggcggttt tagtgctgtt gatctttggg ttgtctgttc cggcagtggc cggtgagggg
     1021 gtagatgtca tgacggccgg ccggttgcga tgcgaatatc tgaataatcc actgggcatt
     1081 gatgcgaatc cgccgcgatt gagctggatc gtagaatccg gggagcgcgg ggcaaagcag
     1141 acggcctatc ggatcctggt ggcgtcgacg cgggaagctc ttgccagcga ccagggcgac
     1201 ctgtgggaca gcgggaaggt ggactctgcg gatagcgtgc tgatttccta cgccggccag
     1261 gcgctgggtt cgcggcagca ctgctactgg aaggtgaagg tctgggatgc ccatggggct
     1321 gagagcgcgt ggagccagcc ggcggaatgg agcatgggcc tcctcgaacc ggcggattgg
     1381 caggggggct acatcggcct ggatgcggcg aaaggtgatc cgcggcaccc ctggttgcgc
     1441 aaaagctttg aactggactc ggcgcccacg gcggcgcgcg tctatgtcaa tccgctggga
     1501 tattacgagc tgcacgtgaa cggcgagctg gtggatgatt cgccgctgac tccggcgatg
     1561 acgcaattca ataagcgctc cttttatctg acccacgatg tgaccggata ccttcggccg
     1621 gggatcaaca gcatcgcgct ctggacgggt tgcggatggt atcgggaagg gctgccgggc
     1681 gtggcctgtg cggggccgct ggtgcgggca caactcgaag cggaattacc gtcgggcgca
     1741 cagatcacca tcgccacgga ttccacgtgg aaagcgcacc caagccccct gacgaccatc
     1801 gatgtccggc atccgggcca ttacggcggt gaacggtacg acgcgaacgc gaatatcccg
     1861 gcatgggcgt cgatcggctt cgacgcgagc gaatggtccg atgccgtcgc ggtagagccg
     1921 gtcccgcaca cggtgtccgc gcaaccggtc gaaaagaatg cggtgctgcg gcgctacgac
     1981 ccggcgcgca tccggtttct gcgcgaaaac gagtggctga tcgattttga cacgaacctg
     2041 gcgggcctgt tcgagattca attcacggga ctggcgcctg gccaggtggt aaagatggaa
     2101 tacgccgact tcctgccggc cgagggcttc gatccgtttg gccaggtcga cgagtatgtc
     2161 gcggtgggcg ggggccgcga aaccttccgg aaccgtttca actaccacgc gttccgctat
     2221 gtgcggatta caggaattga cgtggcgacg cagacggcag ggatcgcggc gtacctggta
     2281 cggacggatt ttccagtgta cgggagcttt gcctgctcca atcccgtgct gacccgcgtc
     2341 tacgatatgg tgcactacac gctggagtgc ctcagcctcg gggggtacat cgtggattgc
     2401 ccgcacatcg aacgcctggg gtacggcggc gatggccagg cgagcacgcc gacggcgttc
     2461 acgctgtttg gcatggggcc tctgtacacg gcatggctgg ccagctggcg ggactgccag
     2521 cgtccggacg gcggcatgcc gcacacggcg ccgaatccgt tccgcgcggg cggcggtccg
     2581 tactggtgcg gatttatcat cgcggcctcg tgggagatgt atcaggactg cggtgacgtg
     2641 cgaatcctgg acgtaaatta tccggccatg cagcaatggc tgggttacgt cgagacctat
     2701 acgaaagaag gcctgttgcg cccctggccg gagacggact atcggagctg gtatctgggc
     2761 gactgggccc ggccggaccg gcgggagaag caggccgagc gctcggttga ccacgtaaac
     2821 aactgttttg tggtgcagtg ttacgattgg atggcgaata tcgccaccgt gctgggccgg
     2881 gccgaagatg cagcgaagta cagggaggag gccgcagcgc gccggaaacg cgttcatgag
     2941 gcgttttacg atgcagcgcg gggcacctac gtggacgata cgcagctcga cctggcctac
     3001 ccgctgctgg cgggagtggt gccggacgaa ctgcgcgacg cgatcgcgaa gcggctcgag
     3061 gagaagattc gggtcgagca caaggggcat atggatgtgg gcctggccgg agtgccgctg
     3121 ctgacggatg ccctgcttca gatggaccgc aacgacctga ttttcggata catcagcaca
     3181 gaaacctatc cgggctgggg ctatatgctc gcccaaggcg cgacgaccac ctgggaacac
     3241 tgggatgcgg agcgttcgca tattcacaac tgctacaacg gcatcggcgc gtggttctat
     3301 cgcgggctgg cgggcattcg gccggatgca tcggctccgg gatatgccca ttttctgttg
     3361 aaaccatctg tgaccggcga tctcacgtgg gtcagcgcac ggcaggatac tgcgcggggc
     3421 gtcatagaaa gcgtgtggcg tatcgaggac ggccagttcg tatgggacgt gcggatcccc
     3481 gcgaatacca ctgccaccgt gatggtcccg acgagcgacc cgggcgcggt ggccgaatcg
     3541 ggcacgcccg tgtcggaagc agacgggctg gtgcaggtgc gttctgaaga tgggcgcgcg
     3601 gtgttcgaag ccgcggcagg ccggtaccgt ttcacggcgc cgttgcagtg acgtcatgca
     3661 atatggagag cattcggggt cagcgcggac gccagtccgc gagggcctgc ccgtacgacg
     3721 cgatgggcgg ctcgccatag gtctgcaggt aatgcgcgtc aaggtatacg gcgaaggtcg
     3781 tgatgtgccg gatgccgcgc gcgccgtatt cgtcgagatc cgcctgcagg acggcctccg
     3841 accacggcag ttttacgtgg ggctctctcc agtttgagaa aagtgaacaa tccagccagt
     3901 actccaggac ctgagcggtt tccgcgggaa agaccttcag attggcgtcg agaagctcga
     3961 ggtgctcttt attctcggcg caatcgggat cattgagcgg gacgtcgaac tttctgcgga
     4021 taggggcgaa ttccagaaag acgcggggat ccggctttac gatgctgggg ggcgtcaggg
     4081 tattcacgta ggccagatgg gcgagttgcg cccgttcatc aaacgcagcc agggcctggg
     4141 caagaaagtt ttcgagaaga agagcctggt ccgcatccgg aaattcacgg catttcgggc
     4201 actggcacca gggcaggccg tcgtcgcccc agaaatagta gcggccggta ctcggcttga
     4261 gaatgcgggc gcccttgact tcgtggccgc caacgctctc gaggtcgagg gcgcccctgg
     4321 aatggacaca aaggttggca tcgggcgtcc gttcgccgtc gtcgttcatg cggaagaact
     4381 cggggcgggt gtcgaagtgc gtgcgcggga gcagatcggc cattgcatga agctcgaatt
     4441 ccacctggag gcccaattcg cggcaggtat ccaggaacat ctcgccgtca cgggtgcgga
     4501 cgaattcgat cacatttcgc gggaacgggt gaagggcaat ggtggtcagt ctcgcgcgtt
     4561 tggcccgttc cggccaattg agggtcaggt ctgccggggt gatgaccaca ccgcgggtgg
     4621 caaacacagg ctgggattcg ggggccgggt tgccggcgac tgtctccatg cgaggtctcc
     4681 cgtgttctgc tgactgcacg catagtatag gcaccgggag gcgggattcc agcggtttgc
     4741 gcatccgccg tccggatttt gcaggggcgg ccccctgtgg ccgcccatcc ggatgacccc
     4801 tgcggctgcg ttaaagaagg gcaggcacag gggcctgccc ctacataaga gaagaacgca
     4861 ggcaagatgc ct
//
LOCUS       DAOPBQ010000256         4871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14593_length_4871_cov_3.651786, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000256 DAOPBQ010000000
VERSION     DAOPBQ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14593_length_4871_cov_3.651786"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PKW60_09040"
     CDS             <1..375
                     /locus_tag="PKW60_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HPJ99616.1"
                     /translation="FILMHPHVVAGAWVGFNDGDLNKMRRVKELEPSLPVFWDWSSGF
                     DLERGLATAREWKFEAIVVYHEAITEAVVKAIADAGFEPGAWTVNDAERMRKLRDMGV
                     YRFYTDYPRRCFETKRAGTGTP"
     gene            complement(385..2265)
                     /locus_tag="PKW60_09045"
     CDS             complement(385..2265)
                     /locus_tag="PKW60_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPJ99617.1"
                     /translation="MALFLVSGLAPAGEDHLVNRVGDIFESIAGSLQQMGKRSEEIVK
                     PPFGPWRDFNTGPMPGSASYPFEELFPVRSPATIIIDNEFGEIRINSWAEQVVQVNAE
                     ISVGAETSELAHQIGRSIDINVTPRGDGISIRTLLPDTREMGRTRIEVNYVLTIPRDA
                     NIVCRNHFGDTLLQNIGGSAAIDSAYGVIELRNIDGEVNVRARGEALQAQGLAKGGTF
                     ELLGCQAEFTNISGNLRATNFMGSVVLRSLGTEATVDITNANGPIHMYVSEFDVPDLQ
                     CEVLFGELRSHIPMEQNVVGDLVYGRNQNLESKRRVALRTSFDEILIVQEGRSEFANP
                     APDDAKLFESVVEKAAACEPGVELVIDAVAGTIRVEAADGSEVQVMARKHVLLQTTEN
                     APAALEALGVTVERLENRIQVQTTARDGLTALGCSYYRVDLLVRAPRTALLTLRAQNG
                     DSIVREFEGPVNIVQTQGRAVAEKCLGPVEITNQSGDVQVLDCAGTATATATKGTITT
                     RSIGGAQQITCTEGKTVADAPLGPVTIRQHGGDVRIIALGEGILGNYDVSTERGDISI
                     VVAQGSDASFYVETTGGEVHCGALPLQGSIEGNVRKFSGRLNQGQHTVALRTTEGNVI
                     ID"
     gene            complement(2318..2989)
                     /locus_tag="PKW60_09050"
     CDS             complement(2318..2989)
                     /locus_tag="PKW60_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99618.1"
                     /translation="MFGHPTRYELTEYAEALVDGKPVSARIGKHLTSCAACKAQVNLL
                     RESFSFLADAPMLDCTPGSAEQILATARLLRKAERRNGRFFRKAFGVARGLACAAGVV
                     LVAAWVFAAAIGESTQVELSTRRPAVSRLTATPAAPSLEELQRKTSEIQSLASAVNAQ
                     SDTPASLWERQHRHMVLALHADRNAALAALKRNPGCLRASRLVTANLEREAQTLRTLY
                     VEQGR"
     gene            complement(3019..3729)
                     /locus_tag="PKW60_09055"
     CDS             complement(3019..3729)
                     /locus_tag="PKW60_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPJ99619.1"
                     /translation="MSEHDRNKGSSAAMTEAVHKTPQARAPLGLRVIEREGLPFPERS
                     DEDLMLSHGEGSDEAFAELLRRHQRGVMNYIFRMVQNRHIAEELTQEVFLALVKNAER
                     YQPTAKFTTYLYTIASNIVSKEWLRRKRRPKFFSMSSLFNRSKDEEFNAIEHFEDENA
                     DVLTSFQRGEVSEAVNEALRHLPEHQREVFVLRRFQDLSYEEISEVTGVPVGTAKSRV
                     VRAEHALRPFLSKFREYL"
     gene            complement(3866..>4871)
                     /locus_tag="PKW60_09060"
     CDS             complement(3866..>4871)
                     /locus_tag="PKW60_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPJ99620.1"
                     /translation="DLLISAGAPPMLRVNGRLYRTRNDALTAEDCKKLIYTALTADQR
                     KKFEEDKELDFSLAVGRKHRFRVNVYYQKQSVTGAFRPIPEKIPSLDKLGLPEIVESL
                     AERKQGLILVTGPTGHGKTTTQASMIDLINKNRECHIITVEDPIEFVHQHKKSIVDQR
                     EVGSDTHGFNEALKYVLRQDPDVILIGEMRDLETIQAALRAAETGHLVMATLHTNDAI
                     QTVDRVIDVFPAAQQQQIRFMLSMTLLAVISQRLIPRADSEGRILASEILLNTTAVAN
                     LIREGKTHQVYSIMETNYKEGMSTMDRSVKALYMEGIISYEDACAHVQNAKTIMDVSS
                     "
BASE COUNT          890 a         1439 c         1530 g         1012 t
ORIGIN      
        1 ttcatcctga tgcatcccca tgtggtcgcg ggcgcctggg tgggcttcaa cgacggcgac
       61 ctcaataaga tgcggcgcgt aaaagaactg gaaccgtcgt tgccggtgtt ctgggactgg
      121 tcatccggct ttgatctgga gcgcggcctg gctaccgccc gggagtggaa atttgaagcc
      181 attgtggttt atcacgaggc gataacggag gccgtggtga aagccatcgc agatgcgggt
      241 tttgaacccg gcgcgtggac cgtcaatgat gcggaacgca tgcgcaaact gcgggacatg
      301 ggggtctacc ggttttatac ggactatccc cggcgctgtt tcgaaaccaa gagagccggc
      361 accgggacgc cttgaggcgg cgcatcagtc gatgatgacg ttgccttcgg tcgtgcgcag
      421 cgccacggtg tgctgaccct ggttgaggcg tcccgaaaac ttgcggacat tcccctcgat
      481 gctgccctgg agcggtaacg cgccgcaatg gacttcgccc ccggtggtct cgacgtaaaa
      541 cgaagcgtcg gagccctgag ccaccacgat gctgatatcg ccccgctcgg tcgacacgtc
      601 gtaattcccc aggatgcctt cgcccagggc gatgatgcgc acgtcgccgc cgtgctggcg
      661 aatggtcacc gggccaagcg gcgcatcggc cacggtcttg ccttccgtgc aggtgatctg
      721 ctgtgcaccc ccaatagagc gggtggtgat ggtgcctttg gtagcggtgg cggtggcggt
      781 tcccgcgcaa tccaggacct gaacgtcccc gctctggttg gtgatctcga ccgggcccag
      841 gcatttttcc gcgacggcgc gcccctgggt ctggacgatg ttcacggggc cttcaaactc
      901 gcgcacgatc gagtcgccat tttgggcgcg cagggtcagg agcgcggtgc gcggtgcacg
      961 caccagcaaa tcgacacggt aataggagca gccgagggcg gtcagaccgt cccgggcggt
     1021 cgtctgcacc tggatgcgat tctcgagcct ttcgaccgtg accccaaggg cttcgagggc
     1081 agcgggggcg ttttccgtgg tctggagaag cacgtgtttg cgcgccatga cctgcacctc
     1141 ggagccatcc gcggcttcca cgcgaattgt ccccgcaaca gcgtcaatga cgagttccac
     1201 gccgggttcg catgcggcgg ccttttcaac gacgctttcg aacagtttgg cgtcgtcagg
     1261 ggcgggattg gcgaattccg agcggccttc ctgcacaatg aggatctcat cgaacgaggt
     1321 gcgcagggcc acgcgccgtt tcgattcgag attctggttg cggccataaa ccagatcgcc
     1381 cacgacattc tgttccatgg ggatgtgaga gcgcagctct ccgaacagca cctcgcactg
     1441 gaggtcggga acgtcaaatt cggagacata catgtggatc ggaccgttgg cgttggtgat
     1501 atcgaccgtt gcctccgttc ccagactgcg gagaaccacg gagcccatga agttggtggc
     1561 gcgcagattg cccgagatgt tagtaaactc ggcctgacac cccagcagct caaaggtgcc
     1621 gcccttggcc agtccctggg cctgcagggc ttcgccgcgg gcccgcacat tgacttcgcc
     1681 gtcgatgttg cgcagttcga tcacgccgta ggcggagtca atggcggcgc tgccgccgat
     1741 gttctgcagc agggtatctc cgaaatggtt ccggcagacg atgttcgcgt cgcgggggat
     1801 ggtcagcaca tagttgactt cgatgcgggt ccggcccatc tcgcgggtgt cagggagcag
     1861 agtgcggatg ctgatgccgt cgccgcgcgg ggttacgttg atgtcgatcg aacggccgat
     1921 ctggtgcgcc agctcggaag tctcggcgcc gaccgagatc tcggcgttga cctgcaccac
     1981 ctgctccgcc caggagttga tgcgaatttc gccgaattcg ttgtcgatga tgatcgtggc
     2041 tggcgagcgc accgggaaca gctcttcaaa aggatacgag gccgaacccg gcatgggacc
     2101 ggtattgaag tcgcgccagg gaccgaacgg cggcttgacg atctcctcgc ttcgcttccc
     2161 catctgttga agcgatccgg cgatcgactc aaaaatgtcg cccacccggt tgacaaggtg
     2221 gtcttccccg gccggggcca ggccggacac aagaaaaagg gccaagaaga gcatcttggc
     2281 ctttgtcgcg cgggagctga acatcatagg tggccgttca acggccctgt tccacgtaaa
     2341 gcgtcctcaa cgtttgtgcc tcgcgttcca ggttcgcggt cacaagacgg cttgcccgga
     2401 gacaaccggg attgcgtttg agggcggcca gggcagcgtt ccggtcggcg tgcagcgcca
     2461 gaaccatgtg gcggtgctgg cgttcccaca ggctggccgg ggtatcggat tgcgcattga
     2521 ccgccgaggc cagactctgg atttccgagg tcttgcgctg cagctcctcc agcgaggggg
     2581 cggcaggcgt tgccgtcagg cggctcacgg caggcctccg ggtgctgagt tcgacctggg
     2641 tggattcgcc gatagcagcg gcaaataccc aggcagccac cagcaccacg cctgctgcgc
     2701 aggccaggcc ccgggccacg ccaaacgctt tgcggaaaaa ccggccattc cgccgctcgg
     2761 cctttcgcag cagccgggcc gtggccagaa tctgttcggc ggacccgggc gtgcagtcca
     2821 gcatgggggc atcggccaga aacgagaaag attcacgcag cagattcacc tgcgctttgc
     2881 acgctgcgca ggaggtcagg tgtttaccga tccgggcgga cactggcttg ccgtctacca
     2941 gtgcctccgc atactcggtc aattcgtagc gtgttggatg cccaaacatg ttgcctaccc
     3001 aatcactacc cggtcgactc agagatattc cctgaacttg gacaaaaacg gtctcaaggc
     3061 atgttccgcg cgcaccacgc gggacttggc ggtgccaacg ggcaccccgg tcacctcgga
     3121 aatttcttcg tacgagagat cctggaaacg ccgcagcaca aacacctcgc gctggtgttc
     3181 gggcagatgc cgcagcgcct cgttgacggc ttccgagacc tcgccgcgct ggaatgacgt
     3241 aagtacgtcg gcgttttcat cctcaaaatg ctcaatggca ttgaattctt cgtccttgct
     3301 gcggttgaac agcgaggaca tgctgaagaa tttcgggcgt cgtttccggc gcagccattc
     3361 tttcgaaacg atatttgagg cgatggtgta cagatacgtg gtgaatttgg ctgtgggctg
     3421 gtagcgctcg gcgtttttga cgagcgccag aaagacttcc tgcgtcagct cttccgcaat
     3481 atgccggttt tgcaccatgc ggaagatgta attcatcacg ccacgctgat gccgcctcaa
     3541 cagttcggca aaggcttcat cggaaccttc gccgtgcgag agcatgaggt cctcatcgga
     3601 ccgttccgga aagggaagac cttcccgttc aatgaccctc agtcccagcg gcgctcgggc
     3661 ttgcggggtc ttgtgaacgg cctcggtcat ggcggcgctg cttcctttgt tgcggtcgtg
     3721 ctcagacatc gtattttatg ctcagtttct caaaaaggtt ccatctggct ttccagccat
     3781 cagggcgatt atacacagat tccctgccgg ctacaaacca tgccacggca caaacagccg
     3841 tctgccacgt ataaatcaca gcaaatcaag aacttacatc catgatggtc ttcgcgttct
     3901 ggacatgcgc acaggcgtct tcatacgaaa tgatgccttc catgtacagc gccttgaccg
     3961 accggtccat ggtgctcatt ccctctttat aattggtctc catgatgctg tacacctgat
     4021 gcgttttgcc ttcgcgtatc aggttggcca ccgcggtggt gttcaggagg atctccgacg
     4081 ccaggatccg gccttcggaa tccgcccgcg ggatgagccg ctgggaaata accgcgagca
     4141 gggtcatgga cagcataaac cggatctgct gctgctgtgc ggcgggaaac acgtcgatca
     4201 cgcggtcgac cgtctgtatg gcgtcattgg tatggagcgt cgccataacg aggtggccgg
     4261 tctcggcggc gcgcagggcc gcctggatgg tctccagatc gcgcatctcg ccgatgagga
     4321 tcacgtcggg gtcctgccgg agcacgtact tgagggcctc attgaacccg tgggtgtcgc
     4381 tgcccacttc gcgctgatcg acaatgcttt tcttgtgctg atgcacaaat tctatcggat
     4441 cctcgacggt gatgatgtgg cattcgcggt ttttgttgat caagtcgatc atggacgcct
     4501 gcgtcgtggt tttgccgtgg ccggtgggcc cggtgaccag gataaggccc tgtttccgct
     4561 ccgcaagaga ttccacgatc tcgggcagcc cgagcttatc cagggacggg atcttctccg
     4621 gaataggccg aaatgcgccg gtgacggact gcttctggta gtagacgttg acgcggaacc
     4681 ggtgtttccg gccgacggcc agggaaaagt cgagttcctt gtcctcctcg aatttcttac
     4741 gctggtcagc ggtgagggcc gtgtaaatga gtttcttgca gtcctcggcc gtgagggcat
     4801 cgtttcgcgt ccggtacagg cggccgttga cgcgcagcat ggggggcgcc cccgcgctga
     4861 tcagcaggtc c
//
LOCUS       DAOPBQ010000257         4848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14665_length_4848_cov_4.117254, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000257 DAOPBQ010000000
VERSION     DAOPBQ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14665_length_4848_cov_4.117254"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKW60_09065"
     CDS             <1..420
                     /locus_tag="PKW60_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99621.1"
                     /translation="PHLFYRPLVLSGRVTGSFDVRVEPGVVMTCEWRTEGHPYLAGPR
                     IQIRGDGTLVAGENALLQIPPKEWVHVEITTELGEKSDGTWRVDVTVPGRETVTAEHI
                     PFGHNDFRHLAWLGFIADADVDGVFYIDNVSLKKAEP"
     gene            417..2444
                     /locus_tag="PKW60_09070"
     CDS             417..2444
                     /locus_tag="PKW60_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPJ99622.1"
                     /translation="MKSLPRNWANFLTLLGGMLCAVNAAGQAVEEDRFTNSLGMEFVR
                     ITPGTFMMGQAEGGDWDEAPAHEVTISKPFHLAATEITNAQYEAFDPEHRAFRGEKGF
                     AKDDDEAVVFVSWHNAVAFCAWLSEKEEKPYRLPTEAEWEYACRAGTGTLYWTGDSLP
                     EVHHKHQEEFWYPVPVGLHVGMAPPNPWGLHDMHGNVEEWCLDWYGPYDAAPQTDPVG
                     FAAGDFRVTRGGSHSTKVAWLRSANRAGALPEDRHWLIGFRVVCGAYPETAPLPSPAP
                     ALWGRDVQQTRHIWAGGPDPGTPYFEGPRRFVKIPPGSTGPLFSQHNHCPALCACPNG
                     DLLAIWYTTVTEPGRELAIAASRLRNGASEWDNASPFWDAPDRNDHASALYWDGRDTL
                     FHLNGISTDATWGKMALLLRTSTDNGATWSHARLVNPAHQLRNMPIAGIFATSDGRLI
                     LPCDAVTIGEGGSAIHLSSDGGATWVDPGAGRPQPVFGAGRTGAWIAGIHAGVTELSD
                     GRFLALGRGNNIDGRMPMSVSTDGGATWTYEASPFPPVSGGQRLVLRRLQEGALLLVS
                     FTDPSSAENPRGLEFTRADGQTFTGYGMFAAVSYDDGATWPHKKLITPGKEAGVFDGG
                     AWTREFTLDATHAEPKGYLASTQTPDGVIHLISSGLYYRFNLAWIETPAGD"
     gene            2610..>4848
                     /locus_tag="PKW60_09075"
     CDS             2610..>4848
                     /locus_tag="PKW60_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPJ99623.1"
                     /translation="MPRGYRWARVSALTLSLLSVGFLIGCDSSAPRQADRISLESSAS
                     GDNQAGLPGQALPSPLRVVVETEVRAGLFGGKGSRHPVSGAEVYFEIQEPARGAVFMR
                     TGQPNVTAMTGADGIAAANVILGDEPGDILILASVDTGHGPDTVEFRVVSGVQIIGTD
                     LETLVGGTIPEFGIQLADDAGRPVPGVDVHFRVEGGVSDSRVHDDHLLTNEDGIAVTS
                     WRLGSEVKRYFAVVEILDRRPGIPEEQRYHVRGLEFTAMALNKTGMALELLGGLALFI
                     LGMKMMSGGLRRIADRRLKQVLQAMTRNRVLALVVGAILTAVVQSSSATTVMTVGFVN
                     AGLMTLQQAIGVVFGANIGTTLTAQILAFELSALSYPAIALGLLLTTAFRKPNVQFFG
                     EAVMGFGMLFLGMTTMSAIVKPLQYSPEFKSWFMMIDCTPAPGALMPADRALICILIG
                     TATTMVIQSSAATVGLVLALCSQGMLSFYTAIPLVLGDNIGTTITAILASLGANRNAK
                     RTALAHTLFNVIGASYMYVLLFVPLWKGQPVFLGLVNDLTPGNVFVGENIARHVANAH
                     SMFNILNCIVMLPFVGALARLCQRIIPVTESDQEGILAYLEPHLLASPSLALQQAVKE
                     VVYMLRRAQKSINDGCEFFHGGPEELETNIMRREEMIDQLQHEITAYLVELSGKELTP
                     AESSLIPALIHAVNDAERIGDHSENLVHLARLRRKGGCAFTEQAVSEVHQLQEMLNGQ
                     FEATYR"
BASE COUNT          926 a         1471 c         1538 g          913 t
ORIGIN      
        1 ccccatttgt tctacaggcc cctggtgctc tctgggcgcg tcacgggcag tttcgacgtg
       61 cgcgtcgagc cgggcgtcgt gatgacgtgt gaatggcgca cggaagggca cccgtacctg
      121 gccggcccgc gcattcagat ccgcggcgac ggaacgcttg tcgcaggtga gaacgccttg
      181 ctgcagattc cgccgaagga atgggttcat gttgagatca caacggagct tggagagaag
      241 agcgacggca cctggcgcgt cgacgtgacc gtaccaggcc gggaaacggt gacggcggaa
      301 cacataccct tcggacacaa tgattttcgc cacctggcct ggctgggctt tattgcggac
      361 gcagacgtgg acggcgtctt ttatattgac aacgtttcgt tgaagaaagc agaaccatga
      421 aatcactgcc acgaaactgg gctaatttcc tgacgcttct gggtgggatg ctgtgcgccg
      481 tgaatgctgc gggccaggcg gtggaggaag accggttcac caattcactt gggatggaat
      541 ttgtccgaat cacccccggg acattcatga tgggtcaggc cgaaggcggc gactgggatg
      601 aagcaccggc ccatgaggtc accatctcga agccgtttca cctcgctgcc acggagatca
      661 ccaacgcgca atacgaagcc ttcgaccccg agcatcgcgc gtttcgcggg gaaaaagggt
      721 tcgcgaaaga cgatgacgag gcggtggtgt ttgtgagctg gcacaacgcg gtggccttct
      781 gcgcctggct ctcagaaaaa gaggaaaagc cataccggct gcccacagag gcggagtggg
      841 aatacgcttg ccgcgcgggc acaggcaccc tctactggac gggcgacagc ctgcccgaag
      901 tgcaccacaa acatcaggag gagttctggt atcccgttcc ggtgggattg catgtgggga
      961 tggcgccgcc aaatccctgg ggactccacg acatgcacgg gaacgtcgag gaatggtgcc
     1021 tggactggta cgggccatac gacgcggccc cacagaccga tcccgtgggt tttgccgcag
     1081 gggatttccg ggtgacccgg ggcggcagtc acagcacaaa agtagcctgg ctgcgctcgg
     1141 cgaaccgggc gggcgcgctg cccgaggaca ggcattggct tatcgggttc cgcgtggtct
     1201 gtggcgcata tccagaaaca gcccctctcc cctcccccgc gcccgcattg tggggccggg
     1261 atgtccagca gacgcggcac atctgggctg gcggccccga tcccggcacg ccctatttcg
     1321 aaggcccgcg acgctttgta aaaatcccac ccggctccac cgggccgctg ttttcgcaac
     1381 ataaccactg cccggcgctg tgtgcctgcc cgaatggcga tctgctggcc atctggtaca
     1441 cgaccgtcac agaaccgggc cgcgaactgg ccatcgcggc gtcgcgcctg cgcaacgggg
     1501 cgagtgaatg ggacaatgcg tcgccgttct gggatgcacc ggaccgcaac gaccatgcca
     1561 gcgccctcta ctgggacggc cgcgacaccc tgttccacct caacgggata agcactgatg
     1621 ccacgtgggg caagatggcg ctccttctcc gcaccagcac cgataacgga gcgacctgga
     1681 gccacgcgcg cctcgtaaac cctgcccacc agttgcgcaa tatgccaatt gcgggcatct
     1741 tcgccacgag cgacgggcgg ctgatccttc cctgtgatgc cgttaccatc ggcgaaggag
     1801 ggtcggccat tcatttgtcc agcgacggcg gcgccacctg ggttgacccc ggcgccggcc
     1861 ggccgcaacc ggtgtttggg gctggacgca ccggggcctg gatcgcggga attcatgcgg
     1921 gcgtgactga gctgagcgat ggccgcttcc tggccctggg ccgcggcaac aacatcgatg
     1981 ggcgcatgcc catgagcgtt tcaacggatg gcggggcgac gtggacctat gaggccagtc
     2041 cgttcccacc cgtgagcgga ggccagcggc tcgtcttgag gcgcttacag gagggcgccc
     2101 tgcttctcgt gtccttcact gaccccagtt cggccgaaaa tccacgcggc ctggaattca
     2161 cgcgagcaga cggccagact ttcacgggtt atggaatgtt tgcggcagtt tcctacgacg
     2221 acggcgccac ctggccgcat aagaagctga ttactcccgg aaaagaggcc ggcgttttcg
     2281 atggcggagc gtggacccgg gaatttaccc tcgatgccac tcacgcggaa cccaagggct
     2341 acctggcgtc gacccagacg cctgacggcg tcatccacct catcagcagc ggcctctatt
     2401 accggttcaa cctggcctgg atagaaaccc cggcaggtga ctgagtgctc aggcagacct
     2461 ccggacaggg gcggcggccc agcagagtgg tttcctgcac ttgaacctcg cccgcgatct
     2521 tggtatcctt tggcggtcgg tccgtccagc gcaacggaca gcactcctac tttttatttt
     2581 gcgggacaac caggaaactg gggggactta tgccacgcgg ttaccgctgg gcgcgtgtca
     2641 gcgcactgac actttcgctt ctaagtgtcg gttttttaat cggttgtgac tccagcgcgc
     2701 cgcgccaggc ggatagaatc tcgctggagt ccagcgccag tggcgacaac caggcgggcc
     2761 ttcctgggca agctttgccc agcccgctcc gagttgtcgt ggagaccgag gtacgggcag
     2821 ggctgttcgg cgggaaaggc tcccgccacc cggtttccgg ggcggaagtg tatttcgaga
     2881 ttcaagagcc tgcccgcggc gcggtattca tgagaaccgg ccaaccgaac gtcacggcca
     2941 tgacgggcgc agacggcatc gcggcagcca atgtcatcct gggggatgaa ccgggagaca
     3001 ttctcatcct cgcgtccgtg gacaccgggc atggccctga taccgtggaa ttccgcgtcg
     3061 tcagcggggt tcagatcatt ggcacggacc tggaaaccct cgttggcggg acaattcccg
     3121 aatttggaat ccaactcgcc gacgacgctg gcaggccggt accgggggtg gatgtccatt
     3181 ttcgcgtgga aggcggcgtc tctgactcgc gagtgcacga tgaccacctg ttgaccaacg
     3241 aggacggcat tgccgtgact tcctggaggc tcggctcaga ggtcaagcgc tattttgccg
     3301 tggtggagat cctggatcgg cggccgggca ttccagaaga acagcgctat catgtccggg
     3361 gcctggaatt taccgcgatg gcgctgaaca agacggggat ggcgctggag ctcctgggcg
     3421 ggctggccct cttcatcctg ggcatgaaga tgatgtcggg cggtctccgg cgcatcgccg
     3481 accggcgcct gaagcaggtg ctgcaggcca tgacacggaa ccgggtattg gcgctggtgg
     3541 taggcgccat tctgacggcc gtagtgcaat cctccagcgc gaccaccgtc atgaccgtag
     3601 gatttgtgaa tgcaggattg atgaccctgc aacaggcaat cggggtggtg ttcggcgcca
     3661 acatcggcac cacactgacg gcacagatcc tcgcctttga attgagtgcg ctgtcgtatc
     3721 ccgctattgc cctgggcctt ctgctgacaa cggccttccg aaaacccaat gttcaatttt
     3781 tcggcgaggc cgtcatgggt ttcggtatgc tgttcctggg catgacgacg atgtcggcga
     3841 ttgtcaagcc gctccagtac agccctgagt ttaaatcgtg gttcatgatg atcgattgca
     3901 cccccgcacc gggcgctctc atgccagcgg accgcgccct catctgcatc ctgattggca
     3961 ccgccacgac gatggtcatt caatcgagcg cggccacggt gggtctggtg ctggctttgt
     4021 gcagccaggg gatgctgtcg ttttacacgg cgatccccct ggtattgggg gataacatcg
     4081 ggaccacgat caccgcgatc ctggcgtcgc tgggagccaa ccgcaacgcg aaacgcacgg
     4141 cgctggcgca tacgctgttc aacgtgattg gcgcgagcta catgtatgtg ctgcttttcg
     4201 tcccgctgtg gaaggggcag ccggtattcc tcggtctggt aaacgatctg actcccggga
     4261 acgtcttcgt gggagaaaac atcgcccgcc acgtggccaa cgcccactcg atgttcaata
     4321 tcttgaactg catcgtcatg ctgccgtttg tgggagcact ggcgcgattg tgccagcgca
     4381 tcatcccggt gaccgagtcg gaccaggaag gcattctggc gtacctggaa ccgcacttgc
     4441 tcgcctcgcc ctccctcgcg ctgcaacagg cggtaaagga agtcgtgtac atgctgcgcc
     4501 gcgcacagaa atcgatcaat gacgggtgcg agttcttcca tggaggcccc gaggagctgg
     4561 aaacgaatat tatgcggcga gaggaaatga tcgaccagct ccagcatgaa atcaccgcgt
     4621 atctggtgga actgtcgggc aaagagctca cgcccgcgga atcctcgctg atccccgcgc
     4681 tgatccacgc ggtcaatgac gcagaacgca tcggcgacca ttcagagaac ctggtccatc
     4741 tggcgcgtct gcgccgaaag ggcggatgcg cattcaccga gcaggctgtc tccgaagtgc
     4801 accagcttca ggagatgttg aacggccagt ttgaggcgac ctaccgca
//
LOCUS       DAOPBQ010000258         4847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14668_length_4847_cov_4.522120, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000258 DAOPBQ010000000
VERSION     DAOPBQ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14668_length_4847_cov_4.522120"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1886
                     /locus_tag="PKW60_09080"
     CDS             <1..1886
                     /locus_tag="PKW60_09080"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPJ99624.1"
                     /translation="LALQSLLPAGSGATIDTNILQTVSISAPAEPDEPIKLATDDLRK
                     YLGRITNQEIDVVERPNSANGFPVYLISRADRPDGNVSPADALRSEDSDAFRLVVRPT
                     EVRIIGNSSRAVLYGAYDWLERLGCRWFGPHEEFVPSLDQATFTELDVEESPSLRWRG
                     LEYITGCDAPIVDWMTKQKLNVAWPEAYRPKDDLSEDPDLFKREAVDEMVRRDLTIFW
                     GGHILNTLFPPEKYADHPEYFALVGGQRLNPDTDEFGRHQLCTSNPEVMRILTENTIR
                     LLQNHEWIDTLFIWGNDTTQWCECEDCLALEPAPDRESPFGGYDRSASYCRMIKIIYE
                     GSLPGMEVSFPGVRDVLPGRKIAFNHYYNLESMPVDSKGDPLKDYLPDRAVLSSVDDY
                     VQCNRHPLRDYAICPRAARRGVEQIAREWNPLYDDNVFWNYYFAGNFTKGQPLLMVHK
                     IASDFQYARSLGIVGTIDNVTLSPPSRSSYEHWRYNMLNLYLYAKAAWNSEVNVDATV
                     ADVIEKYYGPAAGPMAEFWSELERATLHFGMHPDYMPDDAALTTAPAIHEWLWNIRYL
                     VPNQPVYERLVTHLADARILAAKAEDQPMKTDRMIYGERVGILQDYLAFWEITPTEER
                     WTY"
     gene            1971..3884
                     /locus_tag="PKW60_09085"
     CDS             1971..3884
                     /locus_tag="PKW60_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99625.1"
                     /translation="MMVAIVSSNLSIQFHPVLDDFCQVHLVQAAGQVQPRKMLDDVEM
                     IVRSDRWISFDRFDETAEFLRNRLEENGLQAEIEHLPTSPMAGDGRWRIPERWTVRRA
                     HLDLVDPLPMELADWHRQPFSLITWSRATPAEGLAAELVILDRREEIEGLRTGELDRK
                     FVLTRLEVRPIRRRLVEAGALGIITDAGVRFYPEAVLWQKFGWGGWPFDPDDLRCIGF
                     SLSRETGRALRRAISGRKASVHALVDCSFETGTHPMVTGLLKGASAPREEICIVAHAF
                     EPGAGDNASGVATAMEAARLIATQIEMGHWPAPRRSIRLLCGYENYGSFQRLEEGESI
                     RPLAGIDVDVVGVRHELCNGRMTIFRAPACQSAFMDEVAQRTFNAANKLCGANYSMRL
                     RPMMGSGDDLANDPIFGFPLPWVSTFPYRSYHSSADLPSVLCETGLAAAAVGLAGYAY
                     WLAAASDGSVQHLAEQMASKTRRALPGILRRQGPAGVECALQRLSITLESAKAWAGNL
                     SDAASRARLLRSCSRRADALLGDGDWRGAIRKSVAGQHRLADRIVRRIRPLMPMHEAI
                     SPDLCAEYQRLCPSPAALYYANGERDVRTLAGLIALETGQPPDLVRTAEYFDLLSRIG
                     SIEFLQEPNEEPT"
     gene            3881..4671
                     /locus_tag="PKW60_09090"
                     /pseudo
     CDS             3881..4671
                     /locus_tag="PKW60_09090"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
BASE COUNT          953 a         1425 c         1434 g         1035 t
ORIGIN      
        1 gccttgccct gcagtccttg ctccctgctg gatctggtgc gacgatcgac acaaatatcc
       61 tgcaaaccgt atcaatctct gcgcccgccg agccggatga gccgattaag ctggcgaccg
      121 acgatcttcg gaaatacctg ggccggatca ccaatcaaga gatcgatgtt gtcgaaaggc
      181 cgaactctgc taatggtttt cccgtctatc tgatcagtcg tgctgaccgg ccagacggga
      241 atgtctcgcc cgccgatgcg ctcagatcgg aagattccga tgccttccgt ctggtggttc
      301 ggccaaccga agtcaggatc atcggcaatt cttcgcgggc cgtcctttac ggtgcctatg
      361 actggctgga gcgtcttggt tgccgctggt tcggtccaca cgaggagttt gttcccagtc
      421 tggaccaggc gaccttcacg gaactggatg tcgaagaatc gccttcctta cgctggagag
      481 gcttggagta catcacgggg tgcgacgcgc ccatcgtgga ctggatgacg aagcagaagt
      541 tgaatgtcgc ctggccggaa gcctatcgac ccaaggatga tctgagcgaa gatcctgatc
      601 tcttcaagcg cgaggccgtc gatgagatgg tccggcgcga cctgaccatc ttctggggcg
      661 ggcacatcct gaatacactt ttcccccccg agaagtatgc cgatcatccc gagtactttg
      721 ccctggtcgg cggccaacgc ctcaaccctg ataccgatga gttcggccgg catcaactct
      781 gtaccagcaa tcccgaggtc atgcggatcc tgacggagaa tacgatccgc cttttacaaa
      841 atcatgaatg gatcgatacg ctgttcatct ggggcaatga cacgacccag tggtgcgaat
      901 gcgaagattg ccttgccctg gaacccgctc cggatcgaga atcaccattt ggcggttatg
      961 accggtcggc ctcgtactgc cgcatgatca agatcatcta cgagggctcg ctgccgggga
     1021 tggaggtttc cttcccgggg gtacgcgacg tactccccgg acgaaagatc gccttcaacc
     1081 attactacaa ccttgagtca atgcccgtcg actcgaaggg cgatccctta aaagattacc
     1141 taccggaccg cgcggttctt tcgtccgtgg atgactatgt gcaatgcaac cgccatccct
     1201 tgcgcgatta cgcaatctgc ccgcgcgccg ctcgacgcgg cgtggagcag attgcgaggg
     1261 aatggaaccc cctgtatgac gacaatgtct tttggaatta ttatttcgcc gggaatttta
     1321 ccaaggggca gccgctctta atggttcaca aaatcgcctc ggactttcaa tacgcccggt
     1381 ctttggggat cgttggtacc atcgacaatg tcactctttc accaccctca cggtcctcat
     1441 acgaacattg gcgttacaat atgttgaatt tgtatctgta tgcgaaggcg gcctggaatt
     1501 ccgaagtgaa cgtggatgcg acggtcgcag atgtgatcga gaagtactac ggacccgcgg
     1561 ctgggcccat ggctgaattc tggagcgaac tggagcgggc gacgcttcac tttggtatgc
     1621 atccggatta catgccggac gatgccgcgt tgaccaccgc gccggctatt catgagtggc
     1681 tttggaatat tcgctatttg gtcccgaatc agccggtata cgaacgtctt gtcactcatc
     1741 tcgctgatgc acggattctc gcagcgaagg cggaggatca gccaatgaag actgatagaa
     1801 tgatatatgg cgaacgcgtt ggcatacttc aggattatct ggctttctgg gaaatcaccc
     1861 ccaccgaaga aagatggacc tattagcagc gagaggttgg cacggaatct gtagtcgtaa
     1921 ggctcggatg agcagcaggt gaattttagc gtgcttggag ccgaggtgtc gtgatggtcg
     1981 caattgtcag ttcgaatctt tctatacaat ttcatcccgt gctggatgat ttctgccagg
     2041 ttcatcttgt tcaggccgcg ggtcaggtgc agccacggaa aatgctggac gatgtagaaa
     2101 tgatcgtgcg gtcggatcgt tggatttcct ttgaccgctt tgatgaaacg gccgaattcc
     2161 tgcgtaatcg tctggaggag aatggccttc aggcggagat cgaacacctg ccaacttcgc
     2221 cgatggcggg cgatggccgc tggcggattc ccgaacggtg gaccgtccgt cgtgcccacc
     2281 tcgacttagt ggatcctttg cccatggagt tggcagactg gcaccgacag cctttttcat
     2341 taataacgtg gagccgtgcc actccagcgg aaggcttggc ggccgaactg gtcattttgg
     2401 atcggcggga agagattgaa ggcttgagaa cgggcgagct agaccggaaa ttcgtgctga
     2461 cgcggctgga agtccgcccc atccgccgtc gcctcgttga agccggcgcc cttggcatca
     2521 tcaccgatgc cggtgtacga ttttatccgg aagcggttct ctggcagaaa tttggctggg
     2581 gcggctggcc tttcgatccc gatgatttgc gttgcatcgg gttctctctt tcgcgcgaga
     2641 ccggtcgtgc gctccgtcgt gcgataagcg gccgcaaggc ttcggttcat gccctcgtcg
     2701 attgctcctt cgagacgggg acgcatccca tggtgacggg cctgcttaag ggagcatccg
     2761 cgccgcggga ggagatctgc atcgtcgccc atgccttcga gccgggcgcc ggcgataatg
     2821 cctcgggcgt cgccaccgcc atggaagccg cccgcttgat cgcaacccag attgaaatgg
     2881 gtcactggcc tgcgccccgc cgctccatcc gcctgctttg cggctacgaa aactatgggt
     2941 cttttcagcg tctggaagag ggagaatcca tccggcccct ggcggggatt gatgtcgacg
     3001 tcgtcggcgt tcgtcatgag ttgtgcaatg gacgcatgac gatctttcgc gcccccgcct
     3061 gccagtccgc gtttatggat gaggttgccc agcggacctt taacgcggcc aacaaattgt
     3121 gtggcgccaa ttattcgatg cgcctccgcc caatgatggg aagtggtgac gatctggcca
     3181 atgatccaat cttcggtttt ccgctgccat gggtatcgac gtttccctac cgctcctacc
     3241 acagctcggc cgatctccct tccgtcctgt gcgagacggg cctggcagcg gccgctgtcg
     3301 gcctggccgg gtatgcgtac tggctcgccg ccgcttcaga cggaagcgtg cagcacctgg
     3361 cggaacagat ggcgagcaag acacgccgcg ccttgccggg catcctgcgc cgacaggggc
     3421 cggcgggcgt ggagtgcgcg ttgcagcggc tttcaatcac actggagagc gccaaggcat
     3481 gggccggcaa tttgagcgat gcggccagcc gggcgcggct gctccgatcc tgctcacggc
     3541 gtgccgatgc gctcctcggc gacggggact ggaggggcgc aattcgcaag agcgtggcgg
     3601 ggcaacatcg ccttgcggac cgcatcgtgc gccgcatccg gcctctgatg ccgatgcacg
     3661 aggcgatctc gcctgacctg tgtgccgagt atcagcggct ctgcccgtcg cccgccgcgt
     3721 tgtattacgc caatggcgag cgcgacgtcc gcacgcttgc cggcctgatt gcccttgaaa
     3781 ccggtcaacc gcccgatctg gtccgaacgg ccgagtattt cgacctcctt tccagaatcg
     3841 gatccatcga attcttgcaa gaaccgaacg aggagccaac atgagcacgc aatcaactcc
     3901 ggcccgccgg gccgagctga ccaccgaaca actcgagtct tacgaacacg atggcttcct
     3961 cgtgatcgaa aacctcatct cgccggagga actggctgcc ctgcaccagc ggcagtatga
     4021 gtacacgcac ggcgggcgcg atacgagcgc gcttaccatc cagttggaac cgcgcatcca
     4081 acgcggcgag ttgaaggtgg agcatcccgg tgacggcatc cgtaagatcg atggtctgct
     4141 cgaaagcgat gaccttttcc accgactcgg gacgcatccc agtatcgtcg gcgtcctaaa
     4201 gcagatcctc gggccggaca tcaagctcta ccggaacaca cttctgttta agccgcctcg
     4261 cgtcggttcg gccaagggaa tgcatcagga ctcgccttat tggccatcga gccgatggaa
     4321 ctgtgttcgt gctggttcac gctggatgcc gccacgcctg agaacggctg catggggatt
     4381 atacccggcg ggcacgtgcg aggggcgtta ccacacaaat ccgtcaccga tgattacgtg
     4441 atggacgagg cgacatatcg cgaagcagac gtacggcttg tccccatgcc cgccggggca
     4501 ggactgtttt tccactcact gttgccgcac gcgacagcgc cgaacctgtc gaacaaatgg
     4561 cgccgcgcga tcacgatgtc ctacatgtcg gcgcggtcgc gcttcacggg cgaagggaaa
     4621 cgcccgatgt tcatgagaat taccggccag gattatcctg gttgcgtcta atgatctcgg
     4681 ccatccgcta tggcgatgcc cccggcgttg ccttccgcca atattgagct tattcgctga
     4741 gatacaagga gacgtgacat ggctaccagg gaattcccgc tcgaaccggt tcaggtcgac
     4801 cgggtcgaaa cccgatgccg ccggatcgtc acgccgctgc cggtgcc
//
LOCUS       DAOPBQ010000259         4842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14692_length_4842_cov_3.552538, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000259 DAOPBQ010000000
VERSION     DAOPBQ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14692_length_4842_cov_3.552538"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..2721)
                     /locus_tag="PKW60_09095"
     CDS             complement(118..2721)
                     /locus_tag="PKW60_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99626.1"
                     /translation="MRFARFIPFIIVGLLISVGARSAAFREVAIQVGEAAGPVEQRVA
                     RLLQERLAEPGLAPVRLASSVSSTPDTLTILLGRPEHHAALRDLMDAENIAPLTPLAP
                     GAEGFLLQLLPMSPGGTLLAAGIDDRGCLYAAGEILRQATFQDGRICMPDNLRVRTAP
                     AFEVRGTQFGQSGVALSLGKNRQWTPEETQRVILNYALAGANTFEITPGPICDFLASY
                     GLMTLVDGAANLAFERIPEAWQAAESIGRDGFACLSVPEARAYMLEQCEAQFRACPHV
                     DFVRFKGGDGGGCECDRCDPYGKVFIETVAEMAEIVHKYHPDARIFIVNQKFDDADDR
                     ALFDYLQAEPRPWLWAFGYGPGSDATTWQPGHRQTHRMDLFRYPGYGPFALYPREILR
                     ELPPRHQLVYYNEITHWKYAQHGFIQMYPRADRNGDLPPHWSHEIYERRPDQALTKVY
                     SRLTWYAWPRFYHRVFSDLMHYGVGDVTHSSGHHDHFNQWFWQRLLWAPRQTPEEVTD
                     EYCRTWFGPEAAPLMAEAIFLLEEVMEEIPGKPIDRKDSVERYYELVKQAGEAMPAWR
                     RQQDYVWHMYMQKAATDRYTQERVRQQKALQHRVETQLRVSREPVTDEMLTSVLAWFD
                     DLPETETMVSLREEAFALGEISNARFGMCSEGMFNLDHDFIGLGWLKRQLERARAARG
                     DERREAIAMITDYSDPGEGGFYDNLGTGNPAPHVIFGYPYDHGQPYLNEMLDEGNRPS
                     QASMHFTQDEEQGVTLRYRNLDPDAAYRIRFTFVRPWYQERYDSRMNQKAQTIYADDQ
                     VLAENVELPLKMSDFFTYDIPRELTRDGELVIRFERGADVARGDRVSREQWRNSGGWG
                     TLVSEAWLMKR"
     gene            complement(2911..3105)
                     /locus_tag="PKW60_09100"
     CDS             complement(2911..3105)
                     /locus_tag="PKW60_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99627.1"
                     /translation="MTNQAPTVMCCHCGKSVTYHYDEVNHRQHMWLTILTLGLWGPMW
                     LFDIVSKIKICDVCDKPTKE"
     gene            complement(3690..>4842)
                     /locus_tag="PKW60_09105"
     CDS             complement(3690..>4842)
                     /locus_tag="PKW60_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ99628.1"
                     /translation="AMLSIGEGASKEALEQIRKLGSTNILISTVKPVEDEAVSNVRSF
                     MSMYGLLYDDELRIIETFPAVEHTAPVKEVRKEGRLYERALELRVVGTTPVWFDLVQR
                     SFIAGRAILPRDVATHAHVVVLTEYGARRLLATERAIGANLRIGSEFFEVIGIVRSEA
                     EQGGTIQTPDQEVDAYIPLNVARSCFGDMVIRRTSGSREMARVELHQLIVQIRSDEEV
                     EATAAGITRMLERFHKKTDYRISVPLALLRQAEATKRNFNIVLGSIAGISLLVGGIGI
                     MNIMLASVTERTREIGVRRAIGAQRKQIVVQFLIETMVLSTLGGFIGIGIGVSIPWLV
                     TRFSGMPTVVTPDSLILSVGISMAVGIIFGIYPAVRAANLDPIEALRHE"
BASE COUNT          923 a         1410 c         1550 g          959 t
ORIGIN      
        1 ttgacgatat gcggacggca ttccctgccg tccgcatgca ctatgcagaa accccccggg
       61 gcgaatcaat tccgggcgct gatacgccgg atacgcctcg atagttctcc gcgggcgtta
      121 tcgcttcatg agccaggctt ctgaaaccag ggttccccag ccgccggagt tgcgccactg
      181 ctcgcgcgac acgcgatcgc cgcgcgccac gtcagcgccc cgttcaaacc ggatgaccag
      241 ctcgccgtcc cgggtaagtt cccggggaat gtcataggta aagaaatcgc tcattttgag
      301 cggcagctcg acgttttccg cgagaacctg gtcatcggca tagatggtct gggctttttg
      361 attcatccgc gaatcgtacc gttcctggta ccagggccgc acaaaagtga agcggatgcg
      421 gtaggcggcg tcggggtcca gattacggta gcgcaaggtc acgccctgct cttcatcctg
      481 ggtgaaatgc atggatgcct ggctgggccg gttgccttcg tcgagcattt cgttcaggta
      541 cggctggcca tggtcgtagg ggtaaccgaa aatcacgtgc ggcgcagggt tgccggtgcc
      601 cagattgtcg tagaagccgc cttcgcccgg atcgctatag tcggtgatca tggcgatcgc
      661 ttcgcggcgt tcatcgcccc tggccgcgcg tgcgcgttcc agctgccgtt taagccagcc
      721 cagcccgata aaatcgtggt cgaggttgaa catgccttcg ctgcacatcc cgaaccgcgc
      781 attgctgatc tctcccagcg cgaaggcctc ttcccgtaga gagaccatgg tttcggtttc
      841 cggcaagtca tcgaaccagg caagaacgga ggtcagcatt tcgtccgtta cgggctccct
      901 gcttacgcgc agctgcgtct cgacccggtg ctgcagggct ttctgctggc gtacccgctc
      961 ctgcgtatag cggtccgtgg ccgctttctg catatacatg tgccagacat agtcttgctg
     1021 cctgcgccag gcgggcatcg cttcgccggc ctgtttcacg agctcgtaat agcgctccac
     1081 gctgtctttg cggtcgatgg gcttgcccgg tatctcctcc atcacctctt cgaggaggaa
     1141 gatggcttct gccatgagcg gggccgcttc ggggccgaac caggtgcggc agtattcgtc
     1201 cgttacctct tcgggggtct ggcgcggcgc ccagagcagc cgttgccaga accactggtt
     1261 gaagtgatcg tggtggccgc tgctgtgcgt gacatcgcca acgccgtagt gcatgaggtc
     1321 gctgaacacg cgatggtaga accggggcca ggcgtaccac gtgaggcgcg aatagacctt
     1381 cgtgagggcc tgatcggggc ggcgctcgta tatctcgtgg ctccagtgag ggggaaggtc
     1441 gccgttgcga tccgcccggg gatacatctg aataaaaccg tgttgcgcgt atttccaatg
     1501 ggtgatttcg ttgtaataca cgagttgatg gcgcggcggc agttcgcgca ggatctcgcg
     1561 cggatacagg gcgaaggggc cgtagccggg atatcggaac aggtccatgc ggtgcgtctg
     1621 gcggtgtccg ggctgccagg tggtcgcatc ggaaccgggg ccgtacccga acgcccacaa
     1681 ccacgggcgg ggttccgcct gcagataatc aaaaagggcg cggtcgtccg catcgtcgaa
     1741 tttctggttc acaatgaaaa tgcgcgcgtc ggggtggtat ttgtgcacga tctccgccat
     1801 ttcggctacg gtttcgatga agaccttgcc gtagggatcg catcgatcgc attcgcagcc
     1861 gccgccgtcg ccgcccttga aacgcacgaa gtcaacgtgg gggcaggccc gaaactgggc
     1921 ctcgcactgt tcgagcatgt aggcgcgggc ctcaggaacg gacagacagg caaacccgtc
     1981 gcgcccgatg gattcggcgg cttgccatgc ctcagggatt ctttcgaatg ccaggttggc
     2041 tgcgccatcg accagtgtca tcaggccgta cgatgcgaga aagtcgcaga tcgggccggg
     2101 ggtaatctca aaggtgttcg cgcctgcaag cgcgtagttc aggataacgc gctgggtctc
     2161 ttcgggggtc cactgacggt tttttcccag gctcagcgcg acaccgcttt gcccgaattg
     2221 agtgccacgg acctcgaaag cgggcgcggt tcgcacccgg aggttgtcgg gcatacagat
     2281 cctgccgtcc tggaatgttg cctggcgaag aatctctcca gccgcgtaca ggcagccgcg
     2341 atcgtcaatg cctgcggcca gcagcgtgcc gcccgggctc atgggaagca gctgcagcag
     2401 gaatccctcg gctccgggcg cgaggggcgt caatggcgcg atgttctcag catccatgag
     2461 gtcgcgcaag gccgcgtgat gctctgggcg gcccagaagg atggtcagcg tgtctggggt
     2521 ggaggaaacg ctgctggcga ggcgcacggg cgccaggccg ggttcggcga gacgttcctg
     2581 gagcaggcga gcaacccgtt gctcgacggg gccggccgcc tctccaacct gaatggccac
     2641 ctcgcgaaat gccgcgctgc gggctccaac gctgatgaga aggcccacaa tgatgaatgg
     2701 aatgaatcgg gcgaatcgca tgtgaatcac actcccgtgt tgatccggcc ggtgcgtatg
     2761 ccggagattt gcttgcacgg acatccccag acggtgcaga gggatacgta aagagagccg
     2821 cgccggggaa tacgatagca cagccggggg cacgccgtcg cgcctgacgc actccgaacg
     2881 cgcccccggt ttcaaacggc ctctggatgg ctattccttg gtgggtttat cgcacacgtc
     2941 gcagattttg atcttcgaca cgatatcgaa aagccacatc gggccccata gaccaagcgt
     3001 caagatggta agccacatgt gctggcggtg gttgacctca tcgtaatgat acgtaacgga
     3061 ttttccgcaa tgacagcaca tgacggtggg agcttgattt gtcatttctt tatttccttt
     3121 cgcgaacctg acgggcctgc gaacctggtg catgtgtccg ggcgcggccg ttcagcggct
     3181 gagagtcagt gaaaacgcag ctgttgcgcg aaagaaaatc acacctgaag ttggcgtttg
     3241 agaagaacta cggagcggga accgcaagtg ttgcggacgt gccctgtgga gcagggtgta
     3301 tgcagtgtgt ttaacgggaa gccgaacgcc agggcggccg cgccaaacgg gccgtattcg
     3361 aagggggtgc gcaccccttt ccatgtattg gccggcccag gctgtatcaa ggcgatatgg
     3421 ctggaatatg cggtctggaa tgtggccgga tgtggggaat cagtcagtgc aggggatact
     3481 tcaagaaggc aaaccggcac gcacgccaaa gtcagaacgg ccacgatcgc gaccgtccgc
     3541 agcatggggg ggtggaatgc cccagtgtac tcaggtgcct tgcgcatggg agtgagtata
     3601 gcccaaagaa catacgccgg caacgtcaac gcccgtcggc aaccggcgcc cacctcgaca
     3661 acgtacgctt agagggcatt ggccgtcacc tattcgtggc gcaaggcctc gatcggatcc
     3721 aggttcgctg cccggacggc ggggtagatg ccgaagatga tcccgaccgc catgctgatg
     3781 cccacggaga gaataaggct gtcaggcgtg acgaccgttg gcatgccgga gaagcgggtc
     3841 accagccagg ggatggaaac gccgatccca atgccgatga atcctccaag ggtcgacagc
     3901 accatggtct caatgagaaa ttggactacg atctgcttgc gctgcgcgcc gatggcccgc
     3961 ctgacgccga tttcgcgggt ccgttccgtg acagaggcga gcatgatgtt catgatgccg
     4021 atgccgccaa ccaggaggct gatcccggca atggagccga gtacgatgtt gaaattccgc
     4081 ttggtggctt ctgcctgccg cagcagagcc agggggacgc tgatccggta gtcggttttt
     4141 ttgtgaaacc gttcgagcat gcgcgtgatg cccgcggctg tcgcttcaac ttcctcgtca
     4201 ctgcggatct ggacaatcag ctggtggagc tcgacacgag ccatttcgcg cgacccgctt
     4261 gtccggcgga tgaccatgtc gccaaagcag gaccgggcca cattcagcgg gatataggcg
     4321 tcaacttcct gatccggcgt ctgaatcgtt ccgccttgtt cggcttcgct ccggacaatg
     4381 ccgatcacct caaaaaactc gcttccgatg cgcagattgg cgccgatggc gcgctcggtg
     4441 gccagcagcc tgcgcgctcc gtattcggtg agcacgacga cgtgggcgtg ggtcgcgaca
     4501 tcccgcggca aaatggcccg cccggcgatg aacgagcgct ggacaagatc gaaccagacg
     4561 ggggtggttc cgaccacgcg caattcgagc gcgcgctcat acaggcggcc ctctttacgg
     4621 acctctttca cgggcgcggt gtgctcaacc gcgggaaatg tctcgatgat gcgcaactcg
     4681 tcatcgtaca gaaggccata catgctcata aagcttcgaa cgttcgacac cgcttcgtct
     4741 tcgactggtt tgacggtgga gatcaggata ttggtgctgc cgagtttacg gatctgttcg
     4801 agggcttctt tgctggcgcc ctcgccgata gacagcatgg ca
//
LOCUS       DAOPBQ010000260         4823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14749_length_4823_cov_3.399119, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000260 DAOPBQ010000000
VERSION     DAOPBQ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14749_length_4823_cov_3.399119"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKW60_09110"
     CDS             <1..430
                     /locus_tag="PKW60_09110"
                     /inference="COORDINATES: protein motif:HMM:NF021193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HPJ99629.1"
                     /translation="WFYYALYIAVLMLDAVNSPLERPVSRAIAFIFIPLTATILLSSL
                     VVSENQLKRLLGPFATDLRIFTKETLHQLKRLLIFILGGTLLLLGVVMVFTPGPATVV
                     IPLGLAVLATEFVWAKRLLANTVDHTRSLFSRSRHKKDNR"
     gene            complement(438..1658)
                     /locus_tag="PKW60_09115"
     CDS             complement(438..1658)
                     /locus_tag="PKW60_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPJ99630.1"
                     /translation="MPAKGGARPAGRYVGTVARGIRAPIVCRGDAIAEMVVDSVLKAS
                     QAEGFSIRDRDVVGITESIVARAQGNYATVDAIAADLRAKFGGESLGIVFPILSRNRY
                     AVQLKGMARSAKRVVLQLSYPSDEVGNQLMEPDWLDEHGIDPWTDSLTEDQFRQEFGT
                     ACHPFTGVDYVAYYRSLVEAEGAECVILFSNRPETILAYTRNVLACDIHSRARTKRLL
                     TAAGAERVYGLDDILASPIGDSGYHPEYGLLGSNKATEETVKLFPRDCQVVVDRIQEL
                     FRERLDKTVEVLVYGDGAFKDPVGKIWELADPVVSPAFTPGLVGRPSELKLKYLADNQ
                     FAGLTGEALQQAIAQQVREKKGDLVGAMEAQGTTPRRLTDLIGSLCDLTSGSGDKGTP
                     IVHIQGYFDNFAED"
     gene            1989..2405
                     /locus_tag="PKW60_09120"
     CDS             1989..2405
                     /locus_tag="PKW60_09120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPJ99631.1"
                     /translation="MISKTALHTLKAAVVLARDPEQGFHGAGEIAQAIDAPANYLGKM
                     LQSLVRQGILISQKGLGGGFKLARDPKEITLYEVVEVIDNLSRLSGCFLGRSKCSGDN
                     PCPLHKQWGRIRDNYLAMLHDFTLADLASHTHGSLM"
     gene            2523..3242
                     /gene="ric"
                     /locus_tag="PKW60_09125"
     CDS             2523..3242
                     /gene="ric"
                     /locus_tag="PKW60_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HPJ99632.1"
                     /translation="MTTAINPEATVGQLVTENPALSRVFESFGLDYCCGGGKTLTEAC
                     SSRGVDLQTVRQELARVGASADESQPNPADMTLTELADHIEQTHHAYLREVMPVLQTM
                     SAKVANAHAKRDPRYNDVQRVVGELAQELESHSVKEERILFPAIRRLDAGQSLQGFTC
                     GALENPIHVMEMEHDDMGRALAELRTLTDGYAVPDWACNTCRGMLTELERLEKDLHQH
                     IHKENNILFPRTLEREAAITG"
     gene            3310..3603
                     /locus_tag="PKW60_09130"
     CDS             3310..3603
                     /locus_tag="PKW60_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99633.1"
                     /translation="MMWSRGRHETKSRPEALYDKEEFAELLPYLDTLYGVALRFTGDA
                     RDAVALTEKALVGAVVRMHASDRPSPPKMALLGALREAYMHSLETANSSTASA"
     gene            complement(3611..>4823)
                     /locus_tag="PKW60_09135"
     CDS             complement(3611..>4823)
                     /locus_tag="PKW60_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPJ99634.1"
                     /translation="FENAFSAYIPTTPAFSTLLTGCDVVRTQQPSLRPKGPIPEELPL
                     LPALLRPAGYRTVCVGFGSGFYRGFDTYLNFEGWVSWDERPARKAGNLNAVVLPELER
                     LARSSTPFLMLLRYLDPHAPYLPPAPFDTMFYSKNPCVRGNASMKPVFAFKPFADFLR
                     SWMPPGITDKDFVIAQYDGGLAYMDACIQQLVTRLEELGLLENTLLVITSDHGESLYD
                     HEIYFDHHGLYEPTLSVPLIFYWPGRVPRSVRARAYTLHEDLVPTVLDVCGLKHLARG
                     HRFDGKSNRRFFSDTAGSHRTEFYISECTWMRKHGWRTPIWKYWEALEPDFHGKPPVE
                     LYNLVEDPEERHNRAEQEPDIVAMLQNRLRRWERRRERRTGMRSLIHAYRLGTELCIG
                     PIAPAKTPSTR"
BASE COUNT          967 a         1463 c         1426 g          967 t
ORIGIN      
        1 gtggttttat tatgcgctgt atatcgcggt gctgatgctg gatgctgtca acagcccgct
       61 cgagcggccg gtgagccggg cgatcgcatt catattcatt ccgctcaccg caaccatcct
      121 gttgagctcg ctcgtagttt cagagaatca gctgaaacgg ctgcttggcc cgtttgcgac
      181 cgatctgcgg atctttacaa aagaaacgct ccatcaactc aagcggctgc ttattttcat
      241 cctcggcggc accctgctgc tcctgggcgt cgtcatggtc tttacgccag gccccgccac
      301 cgtggttatt ccgctcggcc tcgccgttct cgccacggag tttgtctggg caaagcggct
      361 tctggccaac accgtcgatc acaccaggag cctgttttcg cgctcacgac acaaaaaaga
      421 caacaggtga gtccggctca gtcctcggca aaattgtcga aatagccctg aatatggacg
      481 atgggtgtgc ctttgtcacc gcttccagag gtcaggtcgc acagcgaacc gataaggtcc
      541 gtcaggcgcc ggggtgtggt cccctgtgct tccatggcgc ccacgaggtc gcctttcttc
      601 tcccggacct gctgggcgat tgcctgctgc agggcctctc ccgtgaggcc ggcgaattgg
      661 ttgtctgcca gatattttag cttcagttcg ctgggcctcc ccaccagacc gggcgtgaac
      721 gcgggggaga ccacgggatc cgccagctcc cagattttgc cgacggggtc tttgaacgct
      781 ccgtcgccgt acaccagcac ttctaccgtc ttgtcgagcc gttcacggaa cagttcctgg
      841 atgcggtcga ccaccacctg gcagtcgcgc gggaagagtt ttacagtctc ttcggtcgcc
      901 ttgttggagc ccagcagacc atattccgga tggtagccgc tgtcgccgat gggggaggcg
      961 aggatatcgt ccagcccata cacgcgctcc gcacccgcgg ccgtcagcaa ccgtttggtg
     1021 cgcgctcggc tgtggatatc acaggccagc acgttgcgtg tataggccag gatggtctcg
     1081 gggcggttgg agaagaggat gacgcactcg gcgccttctg cctccacaag agaccggtaa
     1141 tacgcgacat aatccacccc ggtaaacggg tggcaggcag tgccgaattc ttgtctgaac
     1201 tgatcttccg tgaggctgtc cgtccaggga tcgatgccgt gttcgtcaag ccaatccggc
     1261 tccatgagct gattgcctac ttcgtcggac gggtagctga gttgcagcac cacgcgtttt
     1321 gcgctgcgcg ccatgccttt gagctgcacc gcataccggt ttcggctgag aatggggaag
     1381 acgatcccca gggactcgcc gccaaacttc gcacgcaagt ccgcggcgat cgcatccacc
     1441 gtggcgtaat tgccctgtgc ccgcgccacg atcgactcag taatgcccac cacgtctcgg
     1501 tcgcgaatcg aaaaaccctc cgcctgagag gccttcaaaa cgctgtccac gaccatctcc
     1561 gcgatggcat cgcctctgca cacgatcgga gcgcgaatcc ctcgtgccac cgtgcccaca
     1621 taacggcccg ctggtctggc gcctcctttt gccggcatat caaaacgtcc tctctccatg
     1681 cccgttcacg gaagccaggc tctcgtgtgc ctgtctggaa cggtcttact gcaaagagcg
     1741 gtatcataac cgaaaacggg gccgtataca cagatcctcc aggcggccgg atccgcgctc
     1801 catccaccgc ggaaaacacg acacgttttt cggcgctctg caacacctct ggctccggct
     1861 ggatcggtgc gtctccaggc gcatccatcc cgatttttca ggcaaaacag gcctccccag
     1921 acacgccaga ggctttcttg acacggccgg cttaaaatcg tataatcgca tgcgatagag
     1981 gtttaatcat gatcagcaag accgcccttc ataccctcaa agctgccgtg gtgcttgccc
     2041 gggatcccga gcagggcttt cacggggccg gagaaattgc tcaggccatc gacgccccgg
     2101 cgaattatct gggtaaaatg ctgcaatccc ttgtccgtca agggattcta atctcccaaa
     2161 agggtttggg cggggggttc aagctggcgc gggacccgaa ggaaatcacc ttgtatgagg
     2221 tcgtggaggt catagacaac ctgagtcgcc tgtccggttg tttcctaggc cggtcgaagt
     2281 gctctggaga caatccctgc ccgctgcaca agcagtgggg acggatccgc gacaattacc
     2341 tggctatgct gcatgatttc acgctggccg accttgccag ccacacgcac gggagcctga
     2401 tgtgattgcg cgtatttttt tgcggggcat aaaaggatat gcgcatgcga ttagtataga
     2461 tgagggcgcc gcaggccaaa tacgggccct ttgttcctga aacctacaga ggagagtcag
     2521 tgatgacaac tgccatcaac cctgaagcca ccgtcggcca actggtcacc gagaatcccg
     2581 ccctgtcgcg ggtgtttgaa tcgttcgggc ttgactattg ctgtggaggc ggaaagaccc
     2641 tgacagaagc ctgctcgtcc cgcggagtag atcttcaaac tgtgcggcag gagcttgccc
     2701 gcgtgggggc ctccgccgac gaatctcagc ccaatcctgc cgacatgacc ctcacggagc
     2761 ttgccgatca catcgagcag actcaccatg cctaccttcg agaggtgatg ccggtcctcc
     2821 agaccatgtc ggccaaagtc gccaacgccc atgccaaaag agacccgcgc tacaacgatg
     2881 tccagcgggt tgttggagaa ttggcgcaag agctggaaag ccattctgtg aaagaagagc
     2941 gcattctctt ccccgcaata cgccgcctgg atgcgggcca gtccctccag ggcttcacct
     3001 gcggcgcgtt ggagaacccg atccacgtta tggagatgga acatgacgac atggggcggg
     3061 ccctggcgga gctccgtacg ctgaccgacg gttatgcagt tcctgactgg gcctgcaaca
     3121 cctgccgggg tatgttgacc gaactcgaac gcctcgagaa agatctgcac cagcacattc
     3181 acaaagaaaa caacatcctt ttcccgcgaa cactcgaacg ggaagccgcg atcacgggtt
     3241 gactctggcg cacggggctc cgcgggccga atcaacggga ggcgggctcg acggacgcaa
     3301 ggagatgtaa tgatgtggag caggggtcgc cacgaaacga aaagccggcc ggaggcactg
     3361 tacgacaaag aggagtttgc ggaactcctg ccgtatcttg acacactcta cggtgtcgcg
     3421 ttgcgtttca cgggagacgc gcgcgacgcc gtggccctca cggaaaaggc gctggtgggg
     3481 gcggtcgtcc ggatgcacgc cagcgacagg ccatctccgc ccaagatggc gcttctgggc
     3541 gccttgcgcg aggcgtacat gcactcgctc gagactgcga acagcagcac ggccagcgcc
     3601 tgagtgcgtc tcatcgcgtt gagggcgttt ttgctggagc gatggggccg atgcagagct
     3661 cggtgccgag ccggtatgcg tggatcaggc tgcgcatacc ggttctgcgt tctcgccgtc
     3721 tctcccagcg gcgtagacgg ttctgcagca tcgcgacgat atccggctcc tgctctgccc
     3781 gattgtggcg ttcttccggg tcttccacga gattgtacaa ctcgaccggg ggtttcccat
     3841 ggaaatccgg ctccagggcc tcccaatact tccagattgg cgttcgccag ccgtgttttc
     3901 gcatccacgt acattcgctg atgtaaaatt ccgtgcggtg cgagcccgcc gtgtctgaaa
     3961 agaaacgccg gttcgattta ccatcgaacc ggtgaccccg cgccaggtgc ttcaatccgc
     4021 agacatcgag cacggtgggc accaggtctt catgcagcgt ataggcccgg gctcgaacgc
     4081 tacggggcac ccgccccggc caatagaaaa tcagcgggac gctcaaagtc ggttcgtaca
     4141 gcccgtgatg gtcaaagtag atctcatggt cgtagaggct ctcgccgtgg tcgctggtga
     4201 tcacaagcag cgtgttctcg agcaggccga gttcctccag gcgcgtgaca agttgctgga
     4261 tgcacgcatc catgtaggcc agtccgccgt cgtattgggc gatcacaaaa tccttgtcgg
     4321 tgattcccgg cggcatccag gatcggagaa agtctgcgaa cggcttgaac gcaaacacgg
     4381 gtttcatgct cgcattgcct cgtacacagg gatttttcga ataaaacatc gtgtcgaatg
     4441 gagcgggagg gaggtagggg gcgtgggggt ccaggtatcg aaggagcatc aggaaaggtg
     4501 tggaggagcg cgccagtcgt tccagttcgg gcagaaccac cgcattcaga ttcccggctt
     4561 tccgggccgg ccgctcgtcc caggacaccc agccctcgaa attcagatac gtgtcgaaac
     4621 cgcggtaaaa tccgctccca aatccaacgc agaccgtacg gtatcccgcc gggcgcaata
     4681 gcgcgggaag cagcggcagt tcctccggaa tcggcccctt cggccggaga gacggctgct
     4741 gggtccggac aacatcgcag ccagtcagca gggtggaaaa tgcgggtgtc gtggggatgt
     4801 acgcgctgaa cgcgttttca aac
//
LOCUS       DAOPBQ010000261         4820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14760_length_4820_cov_4.417629, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000261 DAOPBQ010000000
VERSION     DAOPBQ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14760_length_4820_cov_4.417629"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PKW60_09140"
     CDS             <1..1220
                     /locus_tag="PKW60_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPJ99635.1"
                     /translation="WVDIAVPKDAPAGAYTGVLTITAKGIDAIELPVTLTVWDFTLPD
                     GPTHENHFGGVDRVHSYLGLERDSDEFHRIEERYEALLAEHRINPAFPRRLWPAIQDD
                     GTTDFSEVDAAISAFVDQFHVTNLEIPRAPFGDILGANREKAKNFMRSWYAYLERKGW
                     ADRSYHYMLDEPNTPEEYETVRKLGALVHEAEPRIRRLVVEQPYTQDPAWGVLDGAFD
                     IWCPLFGFIHEPSVAEKQAQGDEVWSYTALYQRAPDYHPEYEKVKGEDPPYWQIDVPL
                     VSYRIAPWLNRRYGITGLLYWSTCYWGSPDRNPWDEPGFRGYWNGDGALFYPGTDAGL
                     EGPVGSLRLKSLRDGMEDYEYFVILEQRGGAGMVEAIVREAVPTWGTWNQDPGALPRL
                     RERLAEAITTLAP"
     gene            complement(1294..2418)
                     /locus_tag="PKW60_09145"
     CDS             complement(1294..2418)
                     /locus_tag="PKW60_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPJ99636.1"
                     /translation="MIRLAIDAHALGTGAGGNESYIRALLDALSSSTPDIAPVALTPP
                     GWREELPAGMASQALRTQRSWVRVALELPALLRRERFDLFHAQYIAPPACPCPAVVSI
                     HDFVWKRHPATLPPLVRYRLQALIPATLRRARRVFVLCEAMRQELLELYDFPKDRIDV
                     VPPAVHPRFHAVIEPQALDRVRTRHRLPPEYVLYVGALQPRKNLNRLLEAFAAVRREG
                     LPHRLAMVGQEAWMARPLRETIRRLRLEDAIVFTGYVEDADLPALYRGAAGFAYVSIY
                     EGFGIPVAEALACGTPVLTSNNGALAEVAGDAALTRDPFDVDALADGLRTLLTDAELR
                     RRLRDAGPGRARMFSHEAMRAAACQGYRRAVEDTGRLNGR"
     gene            complement(2415..3587)
                     /locus_tag="PKW60_09150"
     CDS             complement(2415..3587)
                     /locus_tag="PKW60_09150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPJ99637.1"
                     /translation="MPKRMNVLYLVRTWAIGGSHTIIFLLLRHLPKDRFNIICVPFDT
                     PSQSDEAFVRALKLRDLPVADDRVPWRSRLGWRKARNAISALIEKYDIDLLHTHDPQS
                     NVLVGLGRHRWPCAVVASPYGWWARMFPLRSHVYNWIERSWALPGVDQIITVSQDMKQ
                     KILRGPTQEDRVNVVYTGLDPAEMQGGAPRAEIRAALGIPDDACVVGTVSRLYVEKGH
                     TFLLDSLSHLADSVPHLHVLIVGEGPLRPALERQAQNAGLDGRVHFTGFYDDLPGALR
                     AMDVFAQPSVLDEGFPTAVLEAQLAGLPVIASDVGGTGETMQPGKTGLLVPPRNVEAL
                     AEAVRTLAEDPDRRMAMSTAGPEWIQRSFTLENMIRQVTGVYEKAVTAYRARSGPS"
     gene            complement(3615..>4820)
                     /locus_tag="PKW60_09155"
     CDS             complement(3615..>4820)
                     /locus_tag="PKW60_09155"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPJ99638.1"
                     /translation="RFRMGWESVPLLALFLWSALGVFLSDTWRLQWPEWFRLGTLVAY
                     YLLSVNLLNSQQRIHAFTMVVIYCGVAMALFAVAQYFIPAFHLQTETAIADIGAGVEG
                     AFVDPESLNGNAAIRVSGRAGHSNWLALIILIIMPLNVYWYAAATSRKARAFCVGAVL
                     LEILALILTFTRTGFLVGLVIFAVLGMRRLVRINPPRLAYVAFAAVLVLLTLPPAYKE
                     RVFDPQKYTRSDSVRHRLQLQDAAWEMMLEKPLWGVGLSGYGLRIVDGDSEVAHIMRW
                     LVDEMHWDPRIIGTHNMYLQLASETGLVGLAFMLLFFGIMLRYLSQAEHFHLRLGNAR
                     AATLIMSLQVSLLSFLLCAVFLHALQQKIWWMMASIAAVVPLYTAGATGRRNDVPVAT
                     ENASFAGES"
BASE COUNT          959 a         1499 c         1535 g          827 t
ORIGIN      
        1 tgtgggtgga tatcgccgtg cccaaagacg cgccggcggg ggcctatacg ggcgtgttga
       61 ccattacggc caaaggcata gacgccatcg aactgccggt gacgctgacg gtctgggatt
      121 tcaccttgcc ggacggccca actcacgaga accatttcgg cggcgtggac cgcgtgcatt
      181 cgtacctcgg cctggagcgt gattccgatg aatttcaccg tatcgaggag cggtacgaag
      241 cgttgcttgc ggaacaccgc atcaacccgg cgtttccgcg ccgtctctgg ccggccattc
      301 aggacgacgg aacaaccgac ttctccgagg tggatgccgc catctcggcg tttgtagacc
      361 agttccatgt gaccaatctt gagattccgc gggcgccctt cggcgacatc ctgggcgcga
      421 accgcgagaa ggcgaagaat ttcatgcggt cgtggtatgc ctacctcgag cggaagggat
      481 gggcagaccg ctcgtaccac tacatgctcg atgagcccaa tacgccggaa gaatacgaga
      541 ccgtacgtaa gctgggagcg cttgtacatg aagccgaacc gcgcatccgc cgcctggtgg
      601 tggaacagcc ctacacgcag gatcccgcct ggggagtcct cgatggcgca ttcgacatct
      661 ggtgcccgct gttcggattc atccacgaac cgagcgtcgc cgaaaaacag gcgcaaggcg
      721 acgaggtgtg gagttatacc gccctctacc agcgggcgcc ggactatcac cccgagtatg
      781 agaaggtcaa aggcgaggat ccgccgtact ggcagatcga tgtcccgctg gtgagttatc
      841 gtattgcgcc gtggctgaac cggcgttacg gcatcaccgg tctgctctac tggtcaacgt
      901 gctactgggg cagcccggat cgcaatccgt gggatgagcc aggttttcgc gggtactgga
      961 acggcgacgg cgcgctgttt tatcccggca cggacgcagg tctcgaaggg cccgtcggct
     1021 cgttgcgtct gaagagcctg cgcgacggca tggaagatta cgagtacttc gtgatcctcg
     1081 aacagcgggg cggagcgggg atggtggaag ctatcgtgcg cgaagcggta ccgacgtggg
     1141 gcacctggaa ccaggacccc ggcgcgctgc cgcgcctccg cgaacggctc gccgaagcca
     1201 tcaccacgct cgcgccatag agatgtccat ctgcttgacg ctggagcgcc tggcggcagc
     1261 gtaatccgtg atgtcccggc tctcgtgccg ggttcatctg ccgttgagac gcccggtatc
     1321 ttcgaccgcg cggcgatacc cctgacaggc ggccgctctc atggcttcgt gggaaaacat
     1381 gcgcgcccgg ccgggtccgg cgtcccgtaa acgccggcgc agctccgcgt cggtaagtag
     1441 ggttcgcagc ccgtccgcga gggcatccac atcgaaggga tcgcgggtca gcgctgcgtc
     1501 tcccgcgacc tccgcgagcg ccccgttatt cgacgtgagt accggcgtcc cgcaggccag
     1561 ggcttccgca acagggatgc cgaaaccttc atagatggaa acgtaggcaa agcccgccgc
     1621 gccgcggtac agcgcgggca gatcggcgtc ttccacgtag cctgtgaaga cgatggcatc
     1681 ctcaagccgc agccggcgta tggtctcccg cagcggcctg gccatccacg cctcctgtcc
     1741 caccatggcg agccggtgag gcaggccttc gcgccgtact gccgcgaagg cctccagtaa
     1801 acggttcaaa ttcttgcgcg gctgcaacgc gcccacatac aaaacatatt ccgggggcag
     1861 ccggtgtctg gtccgcacgc gatccagcgc ctgcggttca atcacggcgt ggaagcgcgg
     1921 atgaacagcg ggtggaacga catcaattcg atctttggga aaatcgtaga gttcgagtaa
     1981 ttcctgacgc attgcctcgc agagcacaaa gacccggcgc gcgcgcctga gcgtggcggg
     2041 aatgagcgcc tgcaggcggt agcggacgag cggcggcaag gttgcgggat gccgtttcca
     2101 tacaaaatcg tgaatgctga cgacggccgg gcacggacag gccggcggcg cgatgtattg
     2161 cgcatgaaag agatcgaacc tttcccggcg caggagggcc ggcagctcca gcgccacccg
     2221 aacccatgag cgctgcgtgc gcagggcttg cgaggccatg ccggcgggca actcctcgcg
     2281 ccagccggga ggagtcaggg ccaccggtgc aatgtccggc gtcgaagaag acagggcatc
     2341 cagcagcgcc cggatatagg attcgttgcc tcccgcaccg gtcccgaggg cgtgggcatc
     2401 gatcgcgagc cgtatcacga gggcccgctc ctcgcgcgat aggccgtgac ggccttttcg
     2461 taaacacccg tcacctgccg gatcatgttt tcgagtgtaa acgagcgctg gatccactcc
     2521 ggtccggcgg tgctcatggc catccggcgg tccggatctt cggccagtgt gcggaccgcc
     2581 tccgccaggg cctccacgtt ccgtggcggc acgaggagcc ctgtttttcc tggctgcatg
     2641 gtttcgccgg taccaccgac atccgaagcg atgacgggca gacccgccag ctgggcttcg
     2701 agcacggccg tggggaatcc ctcgtcaagc acagacggtt gcgcgaacac gtccatcgcg
     2761 cgcagcgctc cggggagatc gtcgtaaaat cccgtgaaat gcacgcgccc gtccagtccg
     2821 gcattctgcg cctgacgttc cagcgccggt cgcagcggcc cctcacccac gatcagcaca
     2881 tgcagatgcg gcacgctgtc cgccaggtgg gacagggaat ccagcagaaa ggtgtggcct
     2941 ttttccacat agaggcggct gaccgtgccc acgacgcagg cgtcgtcggg gatacccagc
     3001 gcagcacgga tttccgcgcg cggcgcgcct ccctgcattt cagcgggatc gagtcccgtg
     3061 tacaccacgt tgacacggtc ttcctgcgtt gggccgcgca ggatcttctg cttcatatcc
     3121 tgcgacaccg taatgatctg gtcaacgccg ggcagggccc aggatcgctc gatccagttg
     3181 taaacatggc tccgcagggg aaacatgcgt gcccaccagc catacggact ggcgacgacc
     3241 gcgcagggcc agcggtgccg gccaaggccc accagcacat tggattgcgg atcgtgtgta
     3301 tggaggagat cgatgtcgta tttttcaatg agcgctgaga tggcgttccg ggctttccgc
     3361 catcccaggc ggcttcgcca cgggacgcgg tcgtccgcga cgggcagatc gcgaagcttc
     3421 agcgcgcgca cgaacgcttc gtcactctgg gaaggggtgt caaacggaac gcagatgatg
     3481 ttgaagcgat ccttcggcag gtgccgaagc aagagaaaga tgatggtgtg cgaacctccg
     3541 atggcccagg tgcggaccag gtacagtacg ttcatgcgtt tgggcatctg cacctccgtg
     3601 catgttgtct ggggtcagga ttcaccggcg aatgaagcgt tttcagtcgc gacaggcaca
     3661 tcgtttcgcc gtcccgtggc gccggcggta tacagcggca cgaccgccgc gatggaagcc
     3721 atcatccacc agatcttctg ctgcagcgcg tgcaggaaga cagcgcacag caggaagctc
     3781 aacaggctta cttgcagcga catgatcagg gttgcggcac gcgcattgcc caatctcaga
     3841 tgaaaatgct cggcctgact caggtagcgc agcatgatcc cgaagaacag aagcatgaag
     3901 gcgaggccta cgagaccagt ttcactggcc agctgaagat acatgttgtg ggtgccgatg
     3961 atgcgtggat cccaatgcat ttcgtcgacg agccagcgca tgatgtgcgc cacttccgag
     4021 tcgccgtcta cgatgcgcaa tccatagccg gacaaaccga cgccccacag cggtttttcg
     4081 agcatcattt cccacgcggc atcctgcagc tggaggcggt gccggacgga atctgaccgg
     4141 gtatatttct gggggtcgaa aacacgttct ttgtaggccg ggggaagggt caggagcacg
     4201 agcacggccg caaacgcgac ataggccagc cggggcgggt tgattcgtac caggcgccgc
     4261 atgcccagga ccgcgaagat cacgaggcct acgagaaatc ccgtgcgggt gaacgtcaga
     4321 ataagggcca atatctccag gagcacggcg cccacgcaaa aagcgcgcgc ctttcgcgac
     4381 gttgccgcag cataccagta cacgttaagc ggcatgatga tcagaatgat aagcgcgagc
     4441 cagttggaat ggcctgcgcg gccgctgacc cggattgccg cgttgccgtt gagcgattcc
     4501 gggtccacaa aagccccttc cacgccggcg ccgatatcgg caatcgccgt ctccgtttgc
     4561 aagtggaacg ccggaatgaa atactgcgcc accgcgaata acgccatcgc tacaccgcag
     4621 tagatgacga ccatcgtgaa cgcatgaatg cgctgctgcg agttcagcag attgaccgaa
     4681 agcaggtagt aggccaccag cgtgcccagg cggaaccatt ccggccattg cagccgccag
     4741 gtgtccgaga gaaacacccc gagtgcgctc cacaaaaaca gcgccagcag gggcaccgat
     4801 tcccagccca tccggaaccg
//
LOCUS       DAOPBQ010000262         4812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14787_length_4812_cov_3.903721, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000262 DAOPBQ010000000
VERSION     DAOPBQ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14787_length_4812_cov_3.903721"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="PKW60_09160"
     CDS             complement(<1..1341)
                     /locus_tag="PKW60_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPJ99639.1"
                     /translation="MKLVAEKMCRFTAVILRDDMPGVLETVARAGILHLTRIEETETW
                     AAALADIDVKRLTRECSERKARLEGLFGDEFHAEAGPWPASVAEVSGKSLAEVDAVIA
                     EAEAAIVPIAESGIRLADRLSSLQSTLSGLDALVPANLPLARLLHSSMLYTVVGVLPQ
                     DQFTKLDARLKDVPSVILPYRRIARELHVVCLVQQKHRETLNRALHDAGFVEAPIPRN
                     TAEITQEARAGLLEEVHTVETRLAENRAALTAAREAAWPAASQTLQRLNAGLLILNIQ
                     DLCKTTDRTCAFAGWVPREQAAPLAEAVNEKAQGRAIVEVVDAETLEPVQAGKIDVPV
                     LFNRPKFLRPFALLTEGFGIPSYTMIDPTIFVAASFLLMFGMMFGDAGHGLVLCGLGA
                     LLIWKTPQFRDIGKLTVYCGIASALFGLLYGSVFGLEELLPTLWTKPLEGIHELF"
     gene            complement(1345..2388)
                     /locus_tag="PKW60_09165"
     CDS             complement(1345..2388)
                     /locus_tag="PKW60_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPJ99640.1"
                     /translation="MRALARYSEANAVIRATLSSLLTKSQLEDILRAGTFADSWNALK
                     STVYGEWLPDMAAPAPLAVERALKDATALRFKRAIRSLHGRATEPGALLLARWELDDL
                     HDALRAWHAKDANYAAFSPGVVLGHAVPFDEIAQADTLETIGGLLSRTPYAKPILETA
                     RVYRETNSLFHLEIALEKGHYRRLVAAIQDLGGQDAGKGLRLIAAEIDLVNLSWLSRC
                     ATYYGDTPAALLREAMIPNVSALASKLEKTGLSPEDISEMSLEYLSELSGGNEGQPPG
                     LERIALLESLVVEEGAEAARRELAGFPFTIACVIALYFLIRTELRNLRGIFAGRAAGF
                     ALEELSSRLSGSR"
     gene            complement(2388..2702)
                     /locus_tag="PKW60_09170"
     CDS             complement(2388..2702)
                     /locus_tag="PKW60_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99641.1"
                     /translation="MKTQIEQILQAEQDAKSRVAAAEQKAREIAAAAAAETSRAAAEF
                     QEEARVQTQALQKDAREQADREKTQVIERARAEAAQIAEREWTGDAARIDAIVRSLAG
                     LS"
     gene            2908..3171
                     /locus_tag="PKW60_09175"
     CDS             2908..3171
                     /locus_tag="PKW60_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99642.1"
                     /translation="MRGFPTAATCSLILGCCLSVQGAARADAVTASPAELAAVTAWAA
                     DVFGGPAAGSFPPAPVEFLLKQQDFSELHFRSSCIDTPICIGA"
     gene            complement(3257..4504)
                     /locus_tag="PKW60_09180"
     CDS             complement(3257..4504)
                     /locus_tag="PKW60_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99643.1"
                     /translation="MKRYFIQQLPAESNPLLTWENGKGFELDGMKGYLRDYSLNQVGP
                     AEVTEAQIAEARQRNMKVYSKADAFAAWQFGTIPYLPFPYQWHERYKALEAYGVNGTL
                     ESWSSGYSPSFMTELRAWYCWSGAPPLDELLRAIAVRNFGAGQEDAVLQAWDHFSRAI
                     RLVPDTGPNMGTNNAVGNPLFFCEPPLRTTTFTHSWTDHGKWMGYLGAQVNPYWPFTV
                     TRMVFYPDFTNRSNLAELYARSATGVEVGPETQLLPVFLKYLRRASDEMEAGLRLYRQ
                     AALASPESKRPVAVREVVVAEQLQRMMQSDAAVLEFEDLRLQQALEPDPVKAQAILDR
                     VETILHEEIARTERSLLAASRDSRLGFQYEQDYVYTPYSLREKIEHLRETAGIHLPAR
                     RRELEAARQQARDGTATAPGENG"
     gene            4595..>4812
                     /locus_tag="PKW60_09185"
     CDS             4595..>4812
                     /locus_tag="PKW60_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPJ99644.1"
                     /translation="MRACQLILKLAGGTLVGGMLDAWATPYQAPVVKLRASRVAAVLG
                     IDVPLQRQMEILSRLELSPRQSGELIECT"
BASE COUNT          889 a         1560 c         1441 g          922 t
ORIGIN      
        1 aaacagttcg tggattcctt ccagcggttt ggtccacaga gtgggcagca gctcctccag
       61 gccaaacaca ctaccgtaca ggagcccaaa gagcgcagag gcgatgccgc agtacacggt
      121 cagcttgccg atgtcccgga actgaggagt cttccaaatc agcaatgccc ccagcccgca
      181 caacaccagc ccatggccgg cgtcgccgaa catcatgcca aacatcaaga gaaacgaggc
      241 cgccacgaag atcgtcgggt cgatcatcgt gtaagaggga atgccgaagc cttccgtgag
      301 cagcgcgaag ggacgcagga atttcgggcg gttgaagagc accggcacgt cgattttgcc
      361 cgcctggacg ggctcgagcg tctccgcatc gaccacctcg acgatggcac ggccctgcgc
      421 cttctcgttg accgcttcgg caagaggcgc tgcctgttcc cgcggaaccc atccggcaaa
      481 ggcgcaggta cggtcggtcg tcttgcacag atcctggatg ttcaggataa gcaggccggc
      541 gttaaggcgc tgcagggttt gagacgcagc gggccaggca gcttcgcggg cggccgtgag
      601 tgcggcacgg ttctcggcca gccgcgtttc cacagtgtgc acttcttcga gcaagccagc
      661 ccgggcttcc tgcgttatct ctgcggtatt ccgtggaatc ggcgcttcga caaagcctgc
      721 atcgtgcagc gctcgattga gggtctctct gtgtttctgc tgcaccaggc ataccacgtg
      781 gagctcccgc gcaatgcggc gatagggcag gatcaccgaa gggacgtctt tcaaccgcgc
      841 gtcgagtttt gtgaattgat cctgaggaag caccccgacc accgtataca gcatcgacga
      901 gtgcagcagc cgggccagag ggagatttgc cggaaccaag gcatccaggc cggacagcgt
      961 cgactgcaga ctggacagtc ggtcggcaag ccggatgcct gattctgcaa tcggcacgat
     1021 cgcggcttcc gcttccgcaa tcacggcgtc cacctcggcc aggcttttcc cggagacctc
     1081 ggccacgctg gcaggccagg gcccggcctc cgcatggaat tcgtcgccga acagcccctc
     1141 cagccgggct ttacgttccg aacactcccg ggtaagccgc ttcacgtcaa tatcggccag
     1201 ggccgcggcc caggtttcgg tttcctcgat gcgggtgagg tgcagaatgc ccgcacgggc
     1261 tactgtctcc agaacgcccg gcatgtcgtc acgcaggatg acggcagtga atcggcacat
     1321 tttttccgcg accagcttca ctcctcacct gctcccgctc agtcggctgg acagctcttc
     1381 cagagcaaac ccggcagccc ggccggcaaa aatcccccgc aaattccgca attcagtacg
     1441 tatcagaaaa taaagtgcaa tcacacaggc aatggtgaat ggaaatcccg cgagctcgcg
     1501 gcgtgctgct tctgcacctt cctctactac gagcgattcg agcagcgcga tccgctccaa
     1561 cccgggcggc tggccttcgt tgccgccaga aagctccgac aggtactcca gcgacatctc
     1621 gctgatgtcc tcgggcgaca agccggtttt ctcaagcttg ctcgcgaggg cggagacgtt
     1681 cgggatcatc gcttcgcgca acagcgccgc gggggtgtcg ccgtaatacg tcgcacaccg
     1741 tgaaagccac gacaggttca ccaggtcgat ctcggccgca atcagccgaa gccctttccc
     1801 ggcatcctgc ccgcccaggt cctggatcgc ggcgaccagc cggcgataat gccctttctc
     1861 cagggcaatc tccagatgga acaggctatt ggtttcccgg taaacgcgcg cggtctccag
     1921 gatgggcttc gcatacggcg tccggctcaa caggccgccg atcgtctcca aggtgtctgc
     1981 ctgcgcgatt tcatcgaacg gcacagcgtg gcccagaaca acgccgggag aaaaggcggc
     2041 ataatttgca tctttggcat gccaggcccg gagcgcgtcg tgcaggtcgt ccagctccca
     2101 gcgggccagc aacagggcgc ccggctccgt cgcccgtcca tgcagcgagc gaatcgcccg
     2161 cttgaagcgc agcgctgttg cgtctttcag ggcccgctcc acggccagcg gcgcaggcgc
     2221 tgccatgtcg ggcagccact cgccgtatac agtcgacttc aacgcgttcc acgaatccgc
     2281 gaacgtcccc gcgcgcagga tatcttccag ctggctcttc gtgagcaggc tggagagggt
     2341 cgcgcgaatg acggcattcg cttcggaata tcgtgccaga gcgcgcatca ggacagtccc
     2401 gcaagggacc ggacaattgc atctatccgg gcggcatccc cggtccactc acgctcggca
     2461 atttgggccg cttcagcacg ggcgcgttcg atcacctgag ttttttcccg gtcggcctgc
     2521 tcacgcgcat ccttttgcag tgcctgggtt tgcaccctgg cttcttcctg aaattcggcg
     2581 gcggcgcgcg aagtctccgc tgctgccgcg gcagcaatct ctcgagcctt ctgctcggct
     2641 gcggcaactc ggctcttggc gtcctgctca gcttgaagta tttgttcgat ttgcgttttc
     2701 actgtctatc ctgcgccggt atgagtcaga agggcaggca ccgcaccatt tcgttatcgc
     2761 aagagacgct gcggcggatt ccgccatttt ggatacgatc gcctattata ctatcaagtg
     2821 tccgtgaaaa gcgtcaacaa acagataaac cgccgggctg ccggtgcttc tttgccgccg
     2881 gcatccggtc aacaggagta ccctcgcatg agaggctttc ccactgccgc tacctgcagc
     2941 ctgattctgg gttgttgcct gtctgtgcaa ggcgccgcac gcgccgatgc cgtcaccgca
     3001 agcccggcgg agcttgccgc cgtcacggcg tgggctgcgg acgtgttcgg gggccctgcc
     3061 gcgggctcat tcccgccggc gccagtcgag ttcctgttaa aacaacagga tttcagcgag
     3121 ctgcatttcc gcagctcctg cattgatacc cccatatgca tcggcgcctg acgatcaaga
     3181 ccacaacgcc cccgacgctc ctcccacaac cagcagtcct ctcccgccgc agcgggtgcg
     3241 catgtcgagg cggtttttac ccgttctcac ccggagcggt tgccgtgccg tccctggcct
     3301 gctggcgcgc agcttcgagt tcccgccggc gcgccggcag atgtatgccg gcagtttcac
     3361 gcaagtgctc gattttttcc cgcagagaat acggcgtata gacgtaatcc tgctcgtact
     3421 ggaaaccgag ccgagaatcc cgggacgccg cgagcagcga acgctccgtc cgcgcaattt
     3481 cttcgtgcag gattgtctcc acccggtcca aaatggcctg cgcctttacc ggatcgggtt
     3541 cgagggcctg ctgcagccgg agatcctcaa attccagcac ggcggcatcg ctttgcatca
     3601 tccgctgcag ctgctcggcc accaccactt cacgcacagc gaccgggcgc ttggattcag
     3661 ggctggcgag cgccgcttgc cggtacagcc gcagcccggc ctccatctcg tcggacgccc
     3721 ggcgcaggta tttcaggaag accggcaaga gctgggtctc cggccccact tcgacaccgg
     3781 tggcgcttcg ggcgtaaagc tcggccaggt tgctgcggtt cgtgaaatcg ggatagaaaa
     3841 ccatccgcgt caccgtgaag ggccagtagg gattcacctg cgcgcccaga taccccatcc
     3901 acttgccgtg gtcggtccag gaatgcgtga atgtcgtggt gcgcagcggc ggttcacaga
     3961 aaaacagcgg atttcccacg gcattgttgg tgcccatgtt cgggccggta tcgggcacga
     4021 ggcggatcgc gcggctgaag tggtcccagg cctgcaatac ggcatcttcc tggcccgctc
     4081 caaaattgcg cacggcgatt gcgcgcagca gctcatccag aggcggggcg ccgctccagc
     4141 agtaccacgc ccgcagctcg gtcatgaaac tcggcgaata cccagaggac cagctttcca
     4201 gcgtcccgtt aactccatac gcttcgagcg ctttgtaacg ctcatgccac tggtacggga
     4261 acgggagata cgggattgtg ccaaactgcc acgcggcaaa cgcgtcggct ttggaataaa
     4321 ccttcatgtt acgctggcgc gcttctgcaa tctgggcttc ggtcacctcg gcggggccaa
     4381 cctggttgag ggaatagtcg cgaaggtaac ccttcatgcc atcgagttca aagcccttcc
     4441 cgttttccca ggtcagcagg gggttggact cggcgggcag ctgctggatg aagtaacgtt
     4501 tcatcgcaac gttgcccggc cgaaaatcga tgttgtattc ccacgggagt atctcgagcg
     4561 cgggattgac ccggccggcg ttgagcgggc ctctatgcgg gcatgccagc tgatcctgaa
     4621 actggcgggc ggcacgctgg taggcggaat gcttgacgcc tgggctacgc cttaccaggc
     4681 gccggtggtg aagctgcgcg ccagtcgcgt tgctgcggtc ttgggaatag acgtgcccct
     4741 tcagcggcag atggaaatcc tctcgcgcct cgagctgagt ccgcgtcagt cgggcgagct
     4801 tatcgaatgc ac
//
LOCUS       DAOPBQ010000263         4811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14792_length_4811_cov_3.597561, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000263 DAOPBQ010000000
VERSION     DAOPBQ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14792_length_4811_cov_3.597561"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKW60_09190"
     CDS             complement(<1..274)
                     /locus_tag="PKW60_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99645.1"
                     /translation="MKRRFTILAVYVLGAAVAACAFAQHPPMPAQAPPERWDREALEL
                     FGALPVQEGGRIKPMATFAAFKLLKLRGIRSWRGPERPGDTRFERML"
     gene            complement(271..1440)
                     /locus_tag="PKW60_09195"
     CDS             complement(271..1440)
                     /locus_tag="PKW60_09195"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPJ99646.1"
                     /translation="MKDYLRTFYRFLSSYGLSCILFLLLFLLTFLGTLEQMHAGLFDV
                     QKKYFESVFLVHELFGVIPVPLPGAYLVMSLLAVNLILGGVVRARKSPAHWGILLGHI
                     GIVVLLLGAAIQYRYSQAGNLTLYEGERSNEFESYYEWELAIADASGNGPFTEYIIPG
                     EQFMHLGTAGTRTFRSPALPFELRVEGAFRNCQPQPVGPGDTPPGRVVDGFTLISLAP
                     DKQAERNAAGLYATLQPASGEAIEAILWGLSPIPLTVEAGGKTWVIDLRKQRFQMPFA
                     VALDQFIHEMHPGTQMPRKFESHVRKIENGVEQSVTISMNAPLRHRGYTLYQASWGPP
                     NAQPGEPLFSVFAVVSNPADQFPLYACVVISLGLCVHYARNLLLYIREQSESAAS"
     gene            complement(1437..2108)
                     /locus_tag="PKW60_09200"
     CDS             complement(1437..2108)
                     /locus_tag="PKW60_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99647.1"
                     /translation="MAVRDRISIILPTLLAVVLASCGGEGSVTEITQTRRVDSLPMPP
                     RPDATSAQRFGYERGMNASAPHGAAHSAEEMELPFTWATPEGWHPAPDRPMRVVTFTA
                     GPAQEAECYVTILGAGGGGTVANINRWREQMGQPPLEEAAVSALPVLEVLGKPSPWVE
                     VRGAFTSASGEAMDDALLLGLVCPADAFSVFVKMTGPATVVEAQRDAFLAFCTSLTWS
                     PPADS"
     gene            2870..3238
                     /locus_tag="PKW60_09205"
     CDS             2870..3238
                     /locus_tag="PKW60_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99648.1"
                     /translation="MFRLGNLNQVVKATKRGYGSVTVAPSSLVTYSCMPVGDLSDAMY
                     TEPDPQAGTRYCLPEGTLLSVQVAQVLAGPEEWCTRGPLVLSESGNDAYHNASFALIH
                     PTEVRVSGGVLNSDKAALTR"
     gene            3262..4155
                     /locus_tag="PKW60_09210"
     CDS             3262..4155
                     /locus_tag="PKW60_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99649.1"
                     /translation="MRLMILVVLLSAPYMKSSPENYMDVMREYESNQRVLRAALENGL
                     ELSDAYDLTIKLMGTSIDPPKRENIAYWEQKDFPFLGFLTQILETTDMPLSRPPYTRP
                     HVSQITVLNWIAVSQHPECTDWLIKYAEELLKKPIESDEDIDRLKGTLKALGYNGSEQ
                     ALDMLFRMQSKKAWENGDYVDLKSYEGSSRKSQQDIVFELRAAALAGIAFSGTERALK
                     AFATGEGLANDMGRFDGIFSIAAHARFGLYNLSWHYQNGLAPEVEAELKSVYDKYHME
                     YRAKEQFGPITDPTRPPRPVM"
BASE COUNT         1014 a         1413 c         1478 g          906 t
ORIGIN      
        1 cgagcatacg ttcaaagcgc gtatcgccgg gccgttcggg accacgccac gaccgaatgc
       61 cgcgcagctt cagcagcttg aacgcggcga acgtcgccat cggcttgatg cggcctccct
      121 cctgcacggg cagcgcgcca aacagctcca gcgcttcccg atcccatcgt tcgggtgggg
      181 cctgcgccgg catgggcgga tgttgtgcga acgcgcaggc cgcgaccgct gcccccaaga
      241 catagaccgc caggatggta aaccgccgct tcatgatgcc gcgctttcac tctgctcgcg
      301 aatgtagagc agcaggtttc gtgcataatg cacacagagg cccaggctga tgactacgca
      361 ggcgtacagc gggaactgat cggccgggtt gctcaccacg gcgaacactg aaaacaacgg
      421 ttcgccgggc tgcgcgttcg gcggccccca cgaggcctgg tacagcgtgt agccacgatg
      481 ccggagaggc gcgttcatgc tgatggtgac actttgttca acgccatttt caatcttgcg
      541 cacatggctc tcgaatttcc gcggcatctg ggtgcccggg tgcatttcat ggatgaactg
      601 gtcgagcgcg accgcaaacg gcatctgaaa ccgttgcttg cggaggtcga tcacccaggt
      661 tttgccgccg gcctccacgg tgagcggaat gggcgacaat ccccagagaa tcgcctcgat
      721 ggcctcgccg gacgcaggct ggagcgtcgc atagagcccc gcagcattgc gttcggcctg
      781 tttgtcgggg gcaagcgaga tgagtgtaaa tccatccacg acccgacccg gaggcgtgtc
      841 gccggggccg accggttggg gctggcaatt gcgaaatgcg ccctcgacgc ggagttcaaa
      901 cggcagggcc gggctgcgga acgtccgcgt gcccgcggtg cccaggtgca tgaactgctc
      961 cccggggatg atatattccg taaaagggcc atttcccgac gcatcggcta tggccagctc
     1021 ccactcgtag tagctctcaa attcgtttga gcgctcgccc tcatacagcg tcagatttcc
     1081 ggcctgggaa taccggtact ggatagccgc gcccagcagc agcaccacga tgccgatgtg
     1141 cccgaggaga atgccccaat gggccgggct cttgcgcgcg cgaacgaccc cgcccaggat
     1201 caggttcacc gccagcagcg acatgaccag ataggctccg ggcagcggta ccgggattac
     1261 gccgaacaac tcgtggacca aaaataccga ttcgaaatat tttttctgga cgtcaaacag
     1321 gcccgcgtgc atctgctcca gcgtgcccag gaaggtcagc agaaataaca acagaaacag
     1381 gatgcatgaa agcccgtacg aggacagaaa tcgatagaag gtgcgcagat agtctttcat
     1441 gagtctgccg gggggctcca ggtgagagat gtgcagaagg ccaggaaggc gtctcgctgc
     1501 gcctcaacca cggttgccgg gcccgtcatc ttgacaaaca cgctgaacgc gtccgccggg
     1561 cacaccagcc cgaggaggag cgcgtcatcc atcgcctcgc ccgatgcgct ggtgaatgcg
     1621 ccccggactt caacccaggg cgatggtttc ccaaggactt caagaacagg cagcgcgctt
     1681 accgcggcct cttccagcgg gggctggccc atctgttcac gccagcggtt gatgttcgca
     1741 accgtgccgc cgccgcccgc gcccaggatg gtgacgtagc attccgcctc ctgggccggg
     1801 ccggcagtga atgtcaccac ccgcatgggg cggtcgggtg ccggatgcca gccttcggga
     1861 gtggcccagg taaacggaag ttccatctct tccgccgaat gggccgcgcc gtgtggcgcc
     1921 gatgcgttca tcccgcgttc atagccgaac cgctgcgcac tggttgcatc gggccggggc
     1981 ggcataggca gagagtccac gcggcgggtt tgcgtgatct cagtgactga accttcgccg
     2041 ccgcacgagg ccagcacgac ggccagcagg gtcggcaaga tgatgctgat acggtctcga
     2101 accgccatga aaatatccag agatatcgcg taatcggact gcgccatgaa tcggacagaa
     2161 gctgtagcct accgtttacc gccgcgcagg tcaaactgag ggcagccgtt tagaatccac
     2221 ccccggccac gcagcgcgta gcgcaggcat cttgcctgcg tgcttctctt tctgagcaca
     2281 ggtccccgtg cctgcctggc caaccccaat ttccgctgcg cgcaggggca gacccttgtg
     2341 cctgcctggt caaccccaat ttccgcagcg cgtaggggca gacccctgtg tctgcccttc
     2401 ttcaaggcag ccgcagcggc tatccggatg ggcaggcaca ggggcctgcc cctacagccg
     2461 gagggcgacc ccacctgtcc actgccgccc acgcgggcca ggggcgcccg gcgcaaaaag
     2521 cctcagcctt ttcctcaaaa acaaaactag cgcacttaga atatggacca aatatgacag
     2581 tcccttgaca taactctaaa tggcagctat aagaaaatga gaacgggcag aagcttcatc
     2641 aacaggggcg caacgatgga ctcatctgcg gggcatatta cggaaaacac catcctcccg
     2701 ggccaataac accttttttc tcgccagaga tccacccgcg cgcctcaatc cagcgaggtt
     2761 tcgcgcgcag cgccgcgaaa tccgggccga ggtccggggc gatacgcggt ggttcaagac
     2821 gtggcatgcg ccgaacacgc tgattgacgg cagccctgag gagagcgagg tgtttcgtct
     2881 gggcaacctg aaccaggtgg tcaaggccac gaagcggggc tacgggagcg tgaccgtcgc
     2941 gccgtcttcg cttgtgacgt attcgtgcat gccggtgggc gatctgtcgg atgcgatgta
     3001 cacggagccg gacccgcagg ccggcacgcg gtattgcctg ccggagggca cgctgctctc
     3061 cgtgcaggtg gcgcaggtgt tggccggtcc ggaggaatgg tgcacgcggg gcccgctggt
     3121 gctgtctgag tccggaaatg acgcgtatca caatgcctcg ttcgcgctca tccatccgac
     3181 ggaggttcgg gtgagcgggg gcgtgttgaa ctctgacaaa gcagctttga cccgataaca
     3241 ctgaaagctc ggaggtggca aatgagattg atgattcttg tggtgctgct gagtgctccc
     3301 tatatgaagt cctctcccga gaattacatg gatgtaatgc gagagtatga atcgaatcag
     3361 cgcgtccttc gcgcagcgct cgaaaacggt ttggagcttt cggacgcgta tgacttgacg
     3421 atcaagctca tgggaacttc gatagaccca cctaagcgcg aaaatattgc atattgggag
     3481 cagaaggact tccccttctt ggggtttctc acgcagatcc tggagacaac tgatatgcct
     3541 cttagtcgtc cgccgtacac gaggcctcat gtgtcgcaaa ttaccgtttt aaactggata
     3601 gccgtgtctc agcatccgga atgcaccgat tggcttatca aatacgcgga agagcttctc
     3661 aagaaaccaa ttgaatccga tgaggatatt gaccgcttaa agggtacatt gaaggcactc
     3721 ggttacaatg gaagtgaaca ggctctggat atgcttttcc gtatgcagag caagaaagcg
     3781 tgggaaaacg gcgattatgt agacctcaaa tcttatgagg gttcttcgcg caaatcccag
     3841 caggatattg tcttcgagct gcgggctgct gcgttggctg gtattgcctt ttcgggcaca
     3901 gaaagggccc tgaaggcttt cgcaacgggc gaaggtcttg ccaacgacat gggcaggttt
     3961 gacggtatat tcagcattgc ggcgcatgcg cggtttggcc tgtataattt atcctggcac
     4021 taccaaaatg gcctggcccc tgaagtcgaa gcagaactga agtccgtata cgataaatac
     4081 catatggaat atcgtgccaa ggagcaattc ggacccatta ccgatcctac gcggcccccc
     4141 aggcctgtaa tgtaaacgca aaatagaaat gtccgctttg cgctgggggt gagcgggtta
     4201 tcctagggct gtgggcaggt gcgtttgtgg gggatgtgtc ggccggtccg gatgagtggt
     4261 gcacacgtgg cccgctgatg ccgcatgaat gcgtgggggc tgaaccgtgt gccgacgagg
     4321 acgtcggcgc acccagacga ggacgtcggc gctcccaggg tacctatcct ccgcgggcgg
     4381 ggctgaaccg tgtgccgacg aggacgtcgg cgctcccagg acgtcggcgc tcccagggta
     4441 cctatggggt ggagttttgg aggttggagt ttgaggctgg attttgaggt gtggcttgca
     4501 gcacccaatc tcacggcaaa aatccccagg cctgacaaag aacaaaggat gtagcgacgc
     4561 acccaggcaa tacccgcaaa tgacggacag agaccgtcgc tgcgcgagtc tgctctgcaa
     4621 caacccataa cggacaaatt ggccgccggc tacgatgaac caacggcgca gccggtcgat
     4681 cagggtgcta ttcgagtctt cggatgcaac ggcctgtatc tttgtctgag ccgcacacgc
     4741 gggataggag tgaggcgccg aaacaatccg cgagtcactc ggtgtcaacc cgcgcattgg
     4801 agtataatcc c
//
LOCUS       DAOPBQ010000264         4799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14828_length_4799_cov_3.576096, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000264 DAOPBQ010000000
VERSION     DAOPBQ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14828_length_4799_cov_3.576096"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PKW60_09215"
     CDS             <1..340
                     /locus_tag="PKW60_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPJ99650.1"
                     /translation="ETNFCTCAADAVKLIERVNHPNFRLLLDTKAMTFEEEDRPALIR
                     KYAKYLAHYHANDANMNGPGFGDVDFGPIFQALREIDYKGYMSVEVFKFELGPETIAS
                     KSLEYLKKFV"
     gene            complement(410..1078)
                     /gene="lipB"
                     /locus_tag="PKW60_09220"
     CDS             complement(410..1078)
                     /gene="lipB"
                     /locus_tag="PKW60_09220"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPJ99651.1"
                     /translation="MKRAIHTIRFDRPMDYAEMLALQKERCAAVEHGTAPNTLYLLEH
                     SPVITLGRKSNPAHVLLSPEELAQKGIALFETDRGGDVTYHGPGQLVAYPILALSAWK
                     ESYRWYLRSLEAVLIDLLRGYGLPAGRMEGFTGIWVRGAKVAAIGVGVHNWVSFHGIA
                     LNVRPDMEHFRFIIPCGIQDKPVVSLETLLTPAPDMAQIMTDFQAAFRAYFEVPDDTP
                     LPEP"
     gene            complement(1092..1754)
                     /locus_tag="PKW60_09225"
     CDS             complement(1092..1754)
                     /locus_tag="PKW60_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPJ99652.1"
                     /translation="MPYGLILDVDGVLADTEAILARATIAMFRELYGVEFAPEDFKPY
                     VGAGSKRYTLGPARMRGLEIDLDAALDKRRENFLAFLQSAESIAFPGAAELIEAAYKH
                     PDWKLAVATSSRSDRAGATLAAAAIDLSRVDAVITGDMVTHMKPDPEIYFTSARIIDV
                     PIQACVVIEDSLVGLEAAKAAGAQCIAVTNTFPREKLSGADRIVDSLTQVDLAMLLAL
                     VT"
     gene            complement(1761..2864)
                     /gene="thrC"
                     /locus_tag="PKW60_09230"
     CDS             complement(1761..2864)
                     /gene="thrC"
                     /locus_tag="PKW60_09230"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPJ99653.1"
                     /translation="MLIEGVINRYRQYLPVTPHTRVITLNEGSTPLIRAANLSAAIHP
                     RLDIFLKYEGLNPTGSFKDRGMTMAITKALEDGFQGVLCASTGNTSASAAAYAARAGI
                     RCAVLIPEGKIAFGKLSQAMIHGARVIQVRGNFDDALRLVRTITDNHPIALVNSINPF
                     RIEGQKTGAFEIIDDFEGVAPDFHVLPVGNAGNITAYWKGYKEYHSAGKTENLPRMLG
                     FQAAGSAPIVLGHPIEKPQTFATAIRIGNPASWKTAEAARDESGGVIDMVTDDEIRAA
                     YRKLAALEGIFAEPASAATIAGIIKYAEKGYFDQFAPFTGGKLRLVCILTGHGLKDPD
                     SAIKTAEQPVTVDAEEEAVLDAMQLAAPNPAGV"
     gene            complement(2868..4148)
                     /locus_tag="PKW60_09235"
     CDS             complement(2868..4148)
                     /locus_tag="PKW60_09235"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPJ99654.1"
                     /translation="MPIGVGVIGAGVVGGGTVQTLLTHRDVIKTRAGVDLALVHVADL
                     DTSRLSEFDLSGVTTSNDAARLIADPNVQIVCELIGGHEPARTFILNALKAGKHVVTA
                     NKMLLASHGPELCRTAIEHGVELRYEASVAGGIPLIRSIREGLAADRIKAIYGILNGT
                     CNYILSRMTEDGHEFDEALRQATALGFAETPPDLDIEGHDTAHKCQILTSLCFSTEVD
                     LDALYVEGITRITHADVAYAGDLGYAIKLLALIQDQGDGIEARVHPTLVPHGHQLAAV
                     RNEFNAVYVESDVAGATLYYGRGAGRFPTASAVVSDVVSIARGLGAPAAPPFVYAEKR
                     PVLDIGATTSRYYLRLTTLDKPGVLGKVCTLLGNYGVSIASCIQQEVRDGKPVPVVLM
                     THETREAELRKALTEIDQLEFTREATHAIRVLQE"
     gene            complement(4490..4726)
                     /locus_tag="PKW60_09240"
     CDS             complement(4490..4726)
                     /locus_tag="PKW60_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HPJ99655.1"
                     /translation="MSYEVTLPSLGEDAADLATVSQWLVKEGDTVAEGDDLVEMTTDK
                     AAFNVPSPVAGTVAEILAAEGQEVRAGGLLCILE"
BASE COUNT          892 a         1392 c         1546 g          969 t
ORIGIN      
        1 cgagaccaat ttctgcacct gcgcggccga tgcggtgaag ctgatcgagc gcgtcaacca
       61 ccccaacttc cgtctgctgc tcgataccaa ggccatgacc ttcgaggagg aagaccgccc
      121 ggcgctcatc cgcaaatacg ccaaatatct tgcccattac cacgcgaacg atgccaacat
      181 gaacgggccc ggtttcggtg atgtggattt cggaccgatt ttccaggccc tgcgcgagat
      241 cgactacaag gggtacatgt cggtcgaggt cttcaagttc gagctcggac cggaaaccat
      301 tgccagcaag agcctcgaat acctcaaaaa attcgtctga taccggtctg gcgctgttcg
      361 cgcccggaag tctggcggcc agcctggcca cgaccctttc gtgtgtgcct cagggctcag
      421 ggaggggtgt atcgtcgggc acttcaaagt aagcgcgaaa cgccgcctga aaatcagtca
      481 tgatttgagc catgtccggc gcgggcgtca ggagagtctc cagcgacacg acgggcttgt
      541 cctgaatgcc gcagggtatg atgaagcgga aatgttccat gtcgggccgg acgttcaggg
      601 caatgccgtg aaaactgacc cagttgtgga ctcccacgcc gatggccgcc accttcgctc
      661 cgcgaaccca gattccggtg aaaccctcca tacgcccggc ggggaggccg tatccgcgca
      721 gcaggtcgat caacacggct tcgagcgagc gcaggtacca ccggtacgat tccttccagg
      781 cggagagcgc gagaatcgga taggccacca gttggcccgg tccgtggtag gtcacgtcgc
      841 cgccgcgatc ggtttcgaac aaggcgatgc ctttttgcgc cagttcttcc ggggacagaa
      901 gcacatgggc cgggtttgat ttgcggccca gggtgatgac gggcgaatgc tcgagcagat
      961 acagcgtgtt gggcgctgtg ccgtgttcca ccgccgcgca gcgctctttc tgaagcgcca
     1021 gcatctccgc ataatccatg ggacggtcga aacgtatggt atgaatcgcg cgtttcatgg
     1081 ccggccgaaa gttatgtcac cagggcaagc agcatggcga gatcgacctg agtcaatgag
     1141 tcaacaatgc ggtcggctcc ggagagtttt tcgcgcggga acgtgttggt gaccgcgatg
     1201 cactgagctc cggccgcttt ggcggcctcg aggccgacca gggaatcttc gataacgacg
     1261 caggcctgga ttggcacatc gatgatccgg gcggacgtga agtagatctc gggatccggt
     1321 ttcatgtggg tcaccatgtc gccggtgatg accgcatcca cgcgggacag gtcgatcgcg
     1381 gcggcggcca gcgtcgcccc ggcccggtca ctccggctcg aggtggctac cgccagtttc
     1441 caatcgggat gcttgtaggc ggcttcgatc agttcggcgg cgcccggaaa cgcaatggat
     1501 tcggcggact gcaggaacgc gaggaaattt tcgcggcgtt tatcgagagc cgcatccagg
     1561 tcgatttcga gcccgcgcat gcgagcgggt cccagcgtgt accgttttga tccggcaccc
     1621 acgtacggct tgaaatcttc cggggcgaac tcaacgccgt acaactcgcg gaacatggca
     1681 atggtcgccc gcgcgaggat cgcttccgta tccgcgagaa cgccatcgac gtccaaaatc
     1741 aggccgtagg gcatggtttg tcacactcct gccgggttgg gagccgccag ctgcattgcg
     1801 tcaagtacgg cctcttcttc ggcgtcgacc gtaaccggct gctcggctgt tttgatagcg
     1861 ctgtcaggat ctttgagtcc gtggccggta agaatgcaga cgaggcgcag tttccccccc
     1921 gtgaagggcg cgaactggtc aaaataacct ttttcagcat atttgatgat gccggcgatt
     1981 gtggcggcgc tggcgggttc ggcgaagatg ccttccagcg cggcgagctt tcgataggca
     2041 gcacgaattt catcgtcggt gaccatgtcg atgacgccgc cggactcgtc acgggcggcc
     2101 tcggcggtct tccagctcgc gggattaccg atgcggatgg ccgtggcgaa ggtctggggt
     2161 ttttcgatcg gatggccgag gacgatgggg gcggagccgg ctgcttgaaa tccgagcatc
     2221 cgcgggaggt tttctgtttt tcccgcagag tggtattcct tgtaaccttt ccagtaggcg
     2281 gtgatgttgc cggcgttgcc gacgggcagc acatgaaaat ccggggcgac gccctcgaaa
     2341 tcgtcgatga tctcgaaggc gccggtcttt tgtccttcga tgcggaacgg attgatggag
     2401 ttgacgaggg cgatgggatg gttgtcggta atggtgcgca ccaggcgcag ggcgtcgtcg
     2461 aaattgcctc gaacctggat cacccgggcg ccatgaatca tggcctgcga cagttttcca
     2521 aacgcgatct tcccctcggg aatgagcaca gcgcagcgta tgcctgcacg ggcggcatag
     2581 gccgccgcgg aggcggaggt gttgccggtg gaggcgcaca acacgccctg aaacccgtct
     2641 tcgagggcct tggtaatggc catggtcatg ccgcggtctt tgaacgaccc ggtgggattg
     2701 aggccctcgt acttcaggaa gatatcgagc cgcgggtgga tcgccgcgct gagattggcg
     2761 gcccggatca ggggggttga cccttcgttg agcgtgataa cccgggtatg cggggtgacg
     2821 ggaaggtact ggcggtaccg gttgatgacg ccttcaatga gcatggttta ctcctgcaac
     2881 acacgaatgg cgtgagtggc ttcgcgcgtg aattcgagct ggtcgatctc cgtcagcgcc
     2941 ttccgtaatt ccgcttcgcg ggtttcgtgg gtcatgagaa caacagggac cggcttgccg
     3001 tcgcgcacct cctgctggat gcatgacgcg atgctgacgc cgtaattgcc caggagggtg
     3061 cacaccttgc ccagcacgcc tggcttgtcc agcgtggtca gacgaagata gtagcgggag
     3121 gtggtggcgc caatatccag tacgggccgt ttctccgcat acacaaacgg cggcgcggcg
     3181 ggggcgccca gtccgcgcgc tatgctcact acatcggaga cgaccgcgct ggctgtcgga
     3241 aaccggcctg cgccgcggcc gtaatagagt gtggcgccgg ccacatcgct ttccacgtac
     3301 acggcgttga attcgttgcg caccgcggcg agctggtgcc cgtgcggtac gagggttgga
     3361 tgaactcgcg cctcgatgcc gtctccctga tcttgaatga gggccaggag tttgatggcg
     3421 tagcccaggt cgccggcgta cgcgacgtcg gcgtgggtga tccgggtgat gccttcgaca
     3481 tacagcgcgt ccagatccac ttccgtcgaa aagcacaggg aagtgagtat ctggcattta
     3541 tgggcggtgt cgtgtccttc tatgtcgaga tcgggcgggg tttctgcgaa tccaagtgcg
     3601 gtagcctggc gcagcgcctc gtcaaattca tgcccgtctt cggtcatgcg gctgaggatg
     3661 tagttgcagg tcccgttgag aatgccgtaa atggctttga tgcggtcggc ggcgagtccc
     3721 tcgcgtatcg accggatgag cgggatgccg ccggctacgg aggcctcata ccgcaattcc
     3781 acgccatgct cgatcgcggt ccggcataat tccgggccgt ggctggccag cagcatctta
     3841 ttggccgtca caacatgttt accggctttc agcgcattga gtatgaacgt ccgggcgggt
     3901 tcgtggccgc cgatgagctc acacacaatc tggacgttcg ggtcggcaat gagccgggca
     3961 gcatcgttgc tcgtagtgac tccgctgagg tcaaattccg acaggcggga cgtatccagg
     4021 tcggccacat ggacgagggc gagatcgacg ccggcacggg tcttgatgac gtcgcgatgg
     4081 gtcagcagag tctgtaccgt gcctcctccg acgactcccg caccgattac tccaacaccg
     4141 ataggcatat tggtcacctt tccatggccg tggcaggcat actgcgcgtt ttctggcgga
     4201 gctccatggc ttgaacacag aggcaggcgc cacatccggt atccccgccg cggggctgcc
     4261 aaggaggctg cgaagcatcg cgtggcgcgg atagcaatga tagcgtacct aaaaccggcg
     4321 attcagttca atgcggcaat ccatcggggc agctgcgcaa aaagcgcagt gccagcaggg
     4381 tacccttcat caatgggact ccctgctgtc ctgaagtcac ttgcgatgca ctgaaccctg
     4441 cccccgggct gtgccggcgg ttaatgatct ggggccaaac gcaggagcgt cattccagga
     4501 tgcagagcag gccgccggcc ctgacctcct ggccctccgc tgcaagaatc tcggccacgg
     4561 tgcctgccac ggggctggga acgttaaatg cggctttgtc ggtagtcatc tcgacgagat
     4621 cgtcgccttc cgcgacggtg tcgccttctt tcaccagcca ctgcgatacg gtggcgagat
     4681 cggcggcgtc ttcgcccaga gacggcaggg ttacttcgta cgacatgcga accttctcct
     4741 tagccctgtt ttcgcggaca cctcattcat ccgtttcagc cgggctaagg agaaggttc
//
LOCUS       DAOPBQ010000265         4798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14831_length_4798_cov_4.153700, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000265 DAOPBQ010000000
VERSION     DAOPBQ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14831_length_4798_cov_4.153700"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="PKW60_09245"
     CDS             complement(<1..1327)
                     /locus_tag="PKW60_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HPJ99656.1"
                     /translation="MGAARHSGCRWGVITVVVGLCLSAAAYEYDSPVYALAQRVEAAA
                     RDAHADATSHNHVLLLEDGYEALLLRVHLIRHARRSIDIQTTILANDECGHLIVSELF
                     DAARRGVVVRLMVDYFMSARDSRWIAALTEAHPNLELKYYRPPAKHAAPPRVLELASV
                     LVRFKGTNQRLHNKLMLFDGLIGIVGGRNIDNHYYNYSTSYNFLDRDSVVLGPATKAM
                     AESFEDYWFYRRTVESRKLTDVAAVIRTGEYQRILRSEDFGVEDFRARMTDGLDAAFI
                     RDRFVTPFQQARRLEYISDAPGKNQGKWLWGGGRAARRIRETIRKTDDELLIQSPYLI
                     LNLSGRRAFRKLQRQEPPAKVIISTNSFASTDNMMAYSANYKMRSLYFERLGFHIFEL
                     KPQPDDFLQLLPNFPELKERAEQDGETREPFVSVHAKSIVVDGRIAYVGS"
     gene            1568..2569
                     /locus_tag="PKW60_09250"
     CDS             1568..2569
                     /locus_tag="PKW60_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015886873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPJ99657.1"
                     /translation="MRYSELGKSGITASVIGMGTWVTGGGMIWGEDPDDAESIRAIHA
                     ALDTGITLIDTAPSYGRGRSEDVVGKAIRDRRDKVVLATKCGLLIDDDRGAFFTEFDG
                     HRMYRSLRPETIRDEVEVSLRRMQTDRIDLYQTHWPAIEPLKTPIADTMACLMNLKAQ
                     GKIRAIGVSNVSLEELKENDAAGDLDGCQFRYSMLARQAESDILPYCLKHNVATLTYM
                     SLEQGLLTGKIGMDRVFSKEEFRSDTGWNPWFAMSHRQRVLDLLAGWSDLTAKYRCTL
                     AQLVLAWTLGQPGVTHVLAGARKTSQIEENVAAADLDIASEDLDRMRRDIVALGEPA"
     gene            2960..3280
                     /locus_tag="PKW60_09255"
     CDS             2960..3280
                     /locus_tag="PKW60_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99658.1"
                     /translation="MAGATFHHFGVPTARQSEKEEFIEGAKVYVTNADEHPFRIEFLR
                     FEKDSPMHPEVQNTPHAAFMVDNLDEALQGRQVIVEPFDANPSLRVAFIKDGDAVLEL
                     MEAK"
     gene            3329..4330
                     /locus_tag="PKW60_09260"
     CDS             3329..4330
                     /locus_tag="PKW60_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HPJ99659.1"
                     /translation="MAMKKFINNPDDLVNELLAGFVLANAGKVALTGNNLVVRAQAKP
                     TDKVAVVTLGGSGHEPALSGFVGEGMLDISVPGEIFAAPGPPRVFEALKLAHRDAGVL
                     LIVLNHEGDVMSAGIAMEMAEKEGLNVKQILTHEDISVPSRQDPKDRRGLVGCFPVIK
                     VAGAAAEQGLPLDECLAIAERMERNMATLAVAVTGATHPSTGQVIAEVPEADMIVGMG
                     QHGEAGGGQQPLKSADETAALMIDALCADLGAKAGDQLLVLINGVGSTTLMEQYLVLN
                     GVKKALDAKNMVLARALVGEFLTVQEMGGFQMCAAKMDEQLLALWDAPCNTPALVVK"
     gene            4348..>4798
                     /locus_tag="PKW60_09265"
     CDS             4348..>4798
                     /locus_tag="PKW60_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HPJ99660.1"
                     /translation="MVTLERLASMVHAAAAQIRASQELLSQLDSHGGDGDHGTTMVRA
                     MNCMEQAVEAKQAGSIPELLKAIGWAVMGVDGGATGPLFGTFFMSMSDAAGGAALDAR
                     GLAGAFAAGLAGVQKRTRAQVGDKTMLDALVPAVQAAQEAADGGAPVE"
BASE COUNT          909 a         1387 c         1610 g          892 t
ORIGIN      
        1 acgagccaac gtaggcgatg cgcccgtcta ccacgatcga tttggcgtgg acggatacaa
       61 acggctcgcg cgtttcgcca tcctgttccg cgcgctcctt aagttcggga aaattcggca
      121 gcagctgcag gaaatcgtcc ggctgcggtt tcaactcgaa aatgtggaag cccagccgct
      181 cgaagtacag cgaacgcatt ttgtagttcg cggaatacgc catcatgttg tccgtcgatg
      241 caaagctgtt ggttgaaatg atgactttcg caggcggttc ctggcgctgc agctttcgga
      301 acgcccggcg tcccgaaagg ttcagaatga gataggggct ctggatcagc agttcgtcat
      361 cggttttccg gatggtctcc cggatgcggc gtgcggctct ccctccaccc cacaaccatt
      421 tgccctggtt cttgccgggg gcgtccgaga tgtattccag ccgccgcgcc tgctgaaacg
      481 gcgtgacgaa gcggtcgcgg atgaacgccg catcgagccc gtcggtcatg cgtgcgcgga
      541 agtcttccac gccgaaatcc tcgctccgga gaatgcgctg gtactcgccc gtccggatca
      601 cggcggccac gtccgtcagt ttgcgactct caaccgttcg ccggtagaac cagtagtctt
      661 cgaacgactc agccatggcc ttggtcgcgg gcccgagcac cacggaatcg cgatcgagga
      721 agttatagct ggtggagtag ttgtaataat gattgtcgat gttgcggccg cctacgatgc
      781 cgatcagacc gtcaaacaac ataagtttgt tgtggaggcg ctggttggtc cccttgaatc
      841 gtaccagcac gctggccagt tccagcaccc ggggcggagc cgcgtgtttc gccggagggc
      901 ggtagtattt cagctcgaga ttggggtgcg cttcggtgag ggcggcgatc catcgtgaat
      961 cccgggcgga catgaaatag tcgaccatga ggcgcaccac cacgccgcgg cgggccgcgt
     1021 cgaacagctc gctcacgatc aagtgtccgc actcgtcgtt cgccagaatg gtggtctgga
     1081 tgtcaatgga acgccgtgca tgccggatca agtgaacgcg caacagcagg gcttcgtacc
     1141 cgtcctcgag caggagcacg tggttgtggc tcgttgcgtc cgcatgcgca tcgcgagcag
     1201 cggcctccac gcgctgggcc agcgcataga ccggtgaatc gtactcgtat gccgcggcgc
     1261 tcagacagag tcccaccacg accgtaatga cgccccaccg gcatccgctg tgtcgtgctg
     1321 cgcccatgcc tccctctcca gccattgctg cgtagggccg attgtgccac aggcgccgcg
     1381 ccacggccaa cccccacggg cctgcgggcc tgtcgcgcct ttcgctactc tgatcggggg
     1441 tggagttcga ggagactgtg cttggtgcgg ccggcgggtc gcgtgcccgg gcgtattggt
     1501 ctataatccg gggcagttca gggtctgcgg aaagacccgt gtccaggata acagaggaac
     1561 acgaatcatg agatacagcg agctcggcaa gtcaggcatc acggcgtcgg tcattggcat
     1621 gggaacctgg gttacgggtg gcggcatgat ctggggggaa gacccggatg acgccgaatc
     1681 cattcgagcg attcacgccg cgctggatac gggcatcacg ctcatcgata ccgcgccttc
     1741 gtacgggcgg ggccgtagcg aggacgtggt cgggaaggcg atccgggacc gccgcgacaa
     1801 ggtggtgctg gccaccaaat gcgggctgct gatcgatgat gatcgcgggg cctttttcac
     1861 ggaatttgac gggcaccgca tgtaccgcag cctgcgtccg gagacgatcc gggatgaggt
     1921 cgaagtgagc ctgcgccgca tgcaaaccga ccggatcgac ctgtatcaga ctcactggcc
     1981 ggcgatcgag ccgttgaaga cgccgatcgc ggacaccatg gcctgtctca tgaacttgaa
     2041 agcgcagggg aagatccggg cgatcggcgt ctcgaacgtc agcctggagg aattgaagga
     2101 aaacgacgcc gcgggcgacc tggacggctg ccagttccgg tacagcatgc tggcccggca
     2161 ggctgagagc gacattctcc cgtattgcct gaagcacaat gtcgcgacgt tgacctatat
     2221 gtcgcttgaa cagggcctct tgaccggcaa aatcggtatg gaccgcgtgt tttctaagga
     2281 ggagttccga agcgacacgg gctggaaccc ctggttcgcg atgtcccacc gccagcgtgt
     2341 cctggacttg ctggccggct ggagcgatct cacggcaaaa taccggtgca cgctggcgca
     2401 actggtcctc gcgtggacac tcggccagcc cggcgtgacc catgtgcttg ccggtgcgcg
     2461 gaagaccagc cagatcgagg aaaacgtggc cgcggccgat cttgatatcg cttcggagga
     2521 tttggaccgc atgcggcgcg atatcgtcgc gctcggagag ccggcctgag gaatccgggg
     2581 cgctggcact cagtggctga tcgccctgcg ccgccgctcc aattcacggg cggttgcgca
     2641 gggattttaa tgcccgtacg gggggtttga cacaccccgg tggccttgtt attctcccat
     2701 cgtgttcgtt gacagaagct ccctatttag tggtttttct gcaaccgggc aatctcctga
     2761 tggctaaggt gcgtatagcg tccctctgcc gggcattgtt ctatctttgg ggtggggttg
     2821 agtggagcgt gcccggaaat ccgccacagg cgccgacctg gctgccgcag ccgggcattc
     2881 cggcggaatg ggcgcaggac gggatggcag actggggttt ttcagggcaa tttgtctgaa
     2941 tatatggagg atgtgaacga tggcaggcgc gacatttcac cattttggcg tacccacggc
     3001 ccggcagtcc gaaaaggaag aatttatcga gggtgcgaag gtctatgtca cgaacgcgga
     3061 tgagcatccg ttccgcattg aatttctgcg ctttgagaag gacagcccga tgcacccgga
     3121 agtccagaat acgccccacg ccgcgttcat ggtcgacaac ctcgacgagg cgctccaggg
     3181 ccgccaggtc attgtcgagc cgtttgacgc caatccatcg ctgcgtgtgg cctttatcaa
     3241 agatggagac gccgtgctcg agctgatgga agccaaataa agcggcggga agccggcgcg
     3301 gacagtaaaa ccaggggggc aagccatcat ggcaatgaag aagttcatca acaatcccga
     3361 tgaccttgtc aacgaactgc tggcgggctt tgtgctggcc aatgcgggca aagtggcgct
     3421 gacgggcaac aaccttgttg tgcgggcaca agcgaagccc acggacaagg tggctgtggt
     3481 cacgctgggc ggcagcgggc acgaacccgc gctgagcggc tttgtgggcg agggcatgct
     3541 cgatatcagc gttccgggcg agattttcgc ggcccccggg ccgccgcgcg tgtttgaggc
     3601 gctcaaactg gctcatcgcg acgcgggcgt gctcctgatc gtcctgaatc acgaaggcga
     3661 cgtcatgagc gccggtatcg cgatggaaat ggccgaaaaa gaaggcctga acgtgaaaca
     3721 aatcctcacg cacgaagaca tctcggtgcc gtcgcggcag gatccaaaag acaggcgcgg
     3781 gctcgtgggc tgtttcccgg tgatcaaggt ggcgggcgcc gccgcggaac agggtttacc
     3841 gctggacgag tgccttgcca ttgcggaacg catggagcgc aacatggcca cgctcgcggt
     3901 ggccgttacg ggtgcgacgc acccctcgac ggggcaggtt atcgcggaag tgcccgaagc
     3961 cgacatgatt gtcggtatgg ggcagcatgg cgaggctggg ggaggccagc agccgctcaa
     4021 gagtgcggac gaaacggccg ccctcatgat tgatgccctg tgcgccgatc tgggggcaaa
     4081 ggccggcgac cagctgctgg tccttatcaa cggcgtcggt tcgaccacgc tgatggagca
     4141 gtacctcgtg ctcaacggcg tgaagaaggc cctggacgcc aaaaacatgg tcctggcgcg
     4201 cgcgctcgtt ggcgagttcc tcaccgtgca ggagatgggc ggttttcaga tgtgcgccgc
     4261 gaagatggac gagcagctgc tggccttgtg ggatgccccc tgcaacacgc cggcgctggt
     4321 cgtgaaataa gtcccgggac agcatacatg gtgactttag agcgactggc ctcgatggtg
     4381 catgcggctg cggcgcagat acgcgccagc caggagctgc tttcacaatt ggactcgcac
     4441 ggcggcgatg gcgaccacgg aacgacgatg gtgcgcgcca tgaattgcat ggagcaggcg
     4501 gtcgaggcga aacaggccgg gtcgatcccc gagctgctga aagccattgg ctgggcggtc
     4561 atgggcgtcg acggcggggc gaccggcccg cttttcggga cgtttttcat gagcatgtcg
     4621 gacgcggccg gcggggcggc gcttgatgcg cggggcctgg ccggggcgtt tgcggcagga
     4681 ttggccggtg ttcagaaacg tacccgcgcc caggtgggcg acaagaccat gctcgatgcc
     4741 ctcgtgccgg ccgtgcaggc tgcgcaggag gccgcagatg gcggcgcccc ggtcgaag
//
LOCUS       DAOPBQ010000266         4793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14844_length_4793_cov_4.010553, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000266 DAOPBQ010000000
VERSION     DAOPBQ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14844_length_4793_cov_4.010553"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PKW60_09270"
     CDS             <1..1115
                     /locus_tag="PKW60_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99661.1"
                     /translation="FNEYNLNVIDNPKTNFIIDDGRNFLLTTRNQYDLITFEPMPMAQ
                     AGVSTFYTREYYESCLAHLAPGGVVSQWVPLHSLNPEIVRSLVYTFTTVFPDYCAWFV
                     NADLFLTGSNQPLVLDMAALRERLENPDVSAALSAAGYHDIPELLASYFMGKEALDAY
                     TQGGAIMTDDRPWAEFVAPKLMYERHVDKILEELIPFFESPVPRLSFDGISSEEAPAL
                     RDAIQRRHDARRIDLQGVKAYYGGMVGGNQEDHFIEALKHDPEDACARHYLLQMVPAV
                     GKQYMRWQEYDRGIELLSKTLPVIPDDLRLVLMLADFHYAKGNRQEALVQYIHYKTLG
                     GDKQRAVLRIQELAREAAPSNAGESGAAPESASSPP"
     gene            complement(1270..4071)
                     /locus_tag="PKW60_09275"
     CDS             complement(1270..4071)
                     /locus_tag="PKW60_09275"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPJ99662.1"
                     /translation="MRRIAIVLLLALLLVAIGCRHLPGRLATGPLNPTRLTCEYLQNP
                     LGIDTAMPRLAWINEAPHPNARAKAQTAYRILAASSLEQLSRDAGDLWDSGRVPGAEA
                     IQIPYGGRPLEARQRVYWKVRVWDESGRLSPWSAPTWFETALLSEDDWSAQWITRKEE
                     PSEDEEALFGDNPAPLFRREFRVDKPVRRARAYVTGLGYYELRLNGEKVGDHWLDPGW
                     TTVSKRVLYSTYDVTDGLRDGSNAVGMVVGNGWYNPLPLLMWNRINIRENLTIGPPRA
                     ILQLDIDYEDGTRDRIVTDETWKAADAPIVRNSVYLGEVYDARLEQPGWDAPGFDDAQ
                     WEAAIPASTAVGPLRTQMAPPIRALRTIQPVSVSEPVPGTYIFDMGENFGGLARLRTK
                     GPAGTRVVLRYGELLHEDGTLNPMTAVCGQIKNRERPADSKRPVTAWQEDVYILKGQG
                     IETYVPRFTFRGFRYLEITGLPAAPARDSVEGLAMHSAVAPAGTFQCANALYNQIQEV
                     TLRTFLSNLFSVQSDCPHREKFGYGGDIVASSEAFLLNFDMAQFYTKAVEDLADAVRP
                     NGGFTETAPFVGIDDAGFGEGSGPIGWGTAHPMLLQQLYQYYGERRLLEAEYERVKAW
                     VGLIESSAPDHIYVGGIGDHESLAEKPELSGTAFYAYNVALAREFALLLGKNDDAARF
                     TALGELIAEAFSTRYLDPETGRYGAGTQASQAFALYLDLVPEAVRASALEILVNDIAV
                     HDGHLSTGIFGTKYMLDVLSTMNRHDVACAITGTRTFPGWGYMLDNGATTLWEHWEGS
                     DNTFSNNHPMFGSVSEWFYKHIAGIRPAPDAVGCDRVLIAPGPPQPGLDWAEASYDSI
                     RGTIRAAWRVEGGWLHLDVSVPVGVSARVLLPVDAQAEVLESGVPAHLAAGVAPVPGN
                     LFCPAFEVASGAYQFSVKQ"
     gene            complement(4137..>4793)
                     /locus_tag="PKW60_09280"
     CDS             complement(4137..>4793)
                     /locus_tag="PKW60_09280"
                     /inference="COORDINATES: protein motif:HMM:NF036710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5010 C-terminal domain-containing protein"
                     /protein_id="HPJ99663.1"
                     /translation="GRCTTAPGKLYLHVFDWPEDALEVPGLKNEVTKACLLTRPDVPL
                     TVTRTGERAVSVALPEHMPNQDATVVVLEISGAPDVDMTIHAAEDGSLTLPAADAQIL
                     GKTARYEAERDAIGFWTEPGDSVSWHITAGQPGKYEVALTYACNKGSGGTPVEIRVGG
                     SAVAASVKETGSWTDFTTHAAGTVEIPSAGVFPVTVSPKNKPVEGVMNLRAVSLRPTQ
                     "
BASE COUNT          889 a         1588 c         1451 g          865 t
ORIGIN      
        1 acttcaacga gtacaacctc aacgtcatcg acaacccaaa aaccaacttc atcatcgatg
       61 acggacgcaa ttttcttctc accacccgca accagtacga cctgatcacg tttgaaccga
      121 tgcccatggc ccaggccggc gtgtcgacgt tttacacccg tgaatactac gagtcctgcc
      181 tggcccacct cgccccgggc ggagtagtct cccagtgggt gccgctccac agcctcaacc
      241 ccgagatcgt ccggtcgctg gtgtatacct tcacgacggt atttccggac tattgcgcct
      301 ggttcgtcaa tgccgacctg tttctcacgg gctccaacca gcccctggtg ctcgacatgg
      361 cagccctgcg cgaacgactc gaaaaccccg acgtgagcgc cgcgctgtcg gccgcgggct
      421 accacgacat ccccgaactg ctggccagct attttatggg gaaggaagcc ctcgacgcgt
      481 acacacaggg cggcgccatc atgaccgacg atcggccctg ggccgaattc gtcgcgccga
      541 aactcatgta cgaacggcat gtcgacaaga tcctcgaaga gctcatcccc tttttcgaga
      601 gcccggttcc gcggctctca ttcgacggaa tttccagcga agaggctccc gcgcttcgcg
      661 acgcgatcca gcgtcgccat gacgcacggc gcatcgacct tcagggggtt aaagcgtatt
      721 acggcggcat ggtcggcggg aatcaggaag atcatttcat cgaggcgctc aaacacgacc
      781 cggaagacgc ctgcgcacgc cattatcttc tgcagatggt gcctgccgtc gggaaacagt
      841 acatgcgctg gcaagagtac gacagaggaa tcgagctgct gtccaagacc ctgccagtta
      901 ttccggatga cctgagactc gtgctgatgc tggcagattt ccattatgcc aagggcaacc
      961 ggcaggaggc gctggtccaa tatatccact acaagacgct cggaggcgac aaacagcggg
     1021 ccgtgctgcg gattcaggaa cttgcccgcg aggccgcacc gtccaacgca ggcgaaagcg
     1081 gggccgcccc cgaatccgca tcatcgcccc cctgacgcgc aagcgaaaca cagaacgcgc
     1141 ccggagctgc catcaaaaag ccgcgttgca gcagctcgcc ccgccgggtc cgtgtttcgg
     1201 cccgacccga tggaggccgg agcacgattc ttcacgaaac gaggctttgc ttgggcgcca
     1261 gtttcccggt tactgtttta cgctgaactg gtaggcgccc gaagcgacct cgaacgccgg
     1321 acagaagaga tttccgggca ccggcgctac ccctgcggcc aggtgcgcgg gcacgccgct
     1381 ttccagcacc tccgcctgtg catccaccgg cagcagaaca cgcgcactca cgccgaccgg
     1441 aacgctgacg tccagatgca gccacccgcc ctccacgcgc cacgcagcac ggatggttcc
     1501 ccggatcgag tcgtacgagg cctcggccca atccaggcct ggctgcggcg ggccgggcgc
     1561 gataagcacg cggtcgcacc ccacggcatc cggcgcaggc cgaatccccg cgatgtgctt
     1621 atagaaccat tcgctcaccg acccgaacat gggatggttg ttcgaaaagg tgttgtcgct
     1681 gccttcccaa tgctcccaga gggttgtggc gccgttatcg agcatgtatc cccagccggg
     1741 aaacgtccgg gtacccgtga tggcgcaggc cacgtcgtgc cggttcatcg tgctcaagac
     1801 gtcgagcatg tactttgtac cgaagatgcc cgtcgagagg tggccatcgt gcaccgcgat
     1861 gtcgttcaca aggatctcga gcgcgctcgc ccgcacggcc tccggcacga ggtccaggta
     1921 gagcgcgaac gcctgcgatg cctgcgtgcc cgcgccgtag cggcctgttt cggggtcgag
     1981 atagcgtgtg ctgaacgcct cggcgatcag ttcgcccagg gcggtaaacc gggccgcatc
     2041 atcgttcttg cccagcaaca gggcaaattc ccgggcaagc gcgacattgt acgcgtagaa
     2101 cgcagtgccc gagagctccg gtttttcggc caggctttcg tggtcgccga tgccgcccac
     2161 gtagatgtgg tccggcgcgc tggattcgat gaggccgacc caggccttca cgcgctcgta
     2221 ctcggcttcg agcagtcgac gttcgccgta atattgatag agctgctgca acagcatcgg
     2281 atgcgccgtg ccccagccga tgggacccga cccttcgccg aatcctgcgt cgtctatgcc
     2341 cacaaacggc gccgtttccg taaatccacc gttgggccgg acggcatcag ccagatcttc
     2401 gaccgccttc gtgtagaact gcgccatgtc gaaattgagc aggaacgcct cgctcgacgc
     2461 gacgatgtcg ccgccgtacc cgaatttctc gcgatgcggg caatcggatt gcacactgaa
     2521 caaattgctt agaaacgtac ggagcgtcac ctcctggatc tggttgtaga gggcattcgc
     2581 acactgaaac gtgcccgcgg gcgcgacagc cgaatgcatg gcgagtcctt ccacgctgtc
     2641 acgtgccggc gcggcgggaa ggccggtaat ttcgaggtag cggaacccgc ggaacgtgaa
     2701 ccgcggcaca tacgtctcaa tgccctggcc tttcaggatg tagacgtcct cctgccatgc
     2761 cgtgacgggc cgttttgaat ccgccgggcg ttcccggttc ttaatctgcc cgcacacggc
     2821 cgtcatggga ttcagggtgc cgtcctcgtg aagcagttcg ccgtagcgca gtacaacgcg
     2881 cgttccagcg ggccctttgg tgcgaagtcg cgccaaaccg ccgaaatttt cgcccatatc
     2941 gaagatatac gttccgggga ccggctcgct cacggatacc ggctggatcg tccgcagcgc
     3001 cctgatcggc ggcgccatct gagtccggag aggcccgacc gccgtggacg ccggaatggc
     3061 cgcttcccac tgcgcatcgt cgaagccggg cgcatcccag cccggctgtt cgagccgggc
     3121 atcgtagacc tcgcccagat agacgctgtt tcgtacgatg ggcgcgtcgg ccgccttcca
     3181 ggtctcgtcg gtcacaatac ggtcccgtgt cccgtcctcg tagtcgatat cgagttgcag
     3241 aatggcccgg ggcggcccaa tggtgagatt ttcgcggatg tttatgcggt tccacatgag
     3301 cagcggcagc ggattgtacc agccgttgcc gaccaccatg cctacggcgt tcgaaccgtc
     3361 ccgcagcccg tccgtcacgt cataggtgga atacaggaca cgtttggaca cagtggtcca
     3421 gccgggatca agccagtggt cgccgacctt ctcgccgttg agccgcaact cgtaataacc
     3481 cagtccggtc acatacgccc gcgcccggcg cacgggcttg tccacgcgga actctctgcg
     3541 aaacagcggc gccggattgt cgccaaacag ggcctcctca tcttcagatg gctcttcttt
     3601 gcgcgtgatc cactgggcgc tccagtcatc ttcgctcagc agggccgtct caaaccacgt
     3661 cggagcgctc cagggcgaca ggcggcccga ctcgtcccat acgcgcacct tccagtacac
     3721 gcgctggcgg gcttcgagcg gccgaccgcc gtacgggatc tggatggctt ccgcccccgg
     3781 cacccggccc gaatcccaga gatcgccggc atcgcggctg agctgttcga gcgaactggc
     3841 cgcaagaatc cggtaggcgg tctgagcctt ggcccgcgca ttgggatgag gcgcttcgtt
     3901 gatccaggcc aggcgaggca tggccgtatc gatacccagt ggattctgca ggtattcaca
     3961 ggtcagccgg gttggattca gaggacccgt tgccagccgt ccgggcagat ggcggcatcc
     4021 gatggccacc aggagcagag ccaggagcaa aacgatagcg atgcggcgca tatacacctc
     4081 cagcggtaat gccgtttccg gaaaatcctg tttgcgcggg ggcgcaacag gctctgttat
     4141 tgcgtagggc gcagcgagac ggcccgcagg ttcatcaccc cttccacagg cttgttctta
     4201 ggcgacaccg tcaccggaaa gacgcccgcg gacgggatct cgacggttcc cgcagcatgg
     4261 gtggtgaaat cggtccagga gccggtttcc ttgacgctgg ccgccacggc ggagccgccc
     4321 acccggatct cgaccggcgt accacccgag cccttgttgc aggcgtaggt aagcgccacc
     4381 tcgtattttc cgggctgccc ggcggtgatg tgccaggaaa ccgagtcccc gggctcagtc
     4441 cagaacccga tggcgtcccg ctccgcctcg taccgggcgg tcttcccgag gatctgggcg
     4501 tctgccgcag gcagcgtgag gctgccgtct tcggcggcat gaatggtcat atcgacgtcc
     4561 ggcgcgccgc tgatctcgag cacaacgacc gtcgcgtcct gattgggcat gtgttcgggc
     4621 agcgccacgg agacggcccg ctcgcctgtc cgcgttacgg tcaaaggcac gtccgggcgg
     4681 gtcaggaggc aggcctttgt gacttcgttc ttcaatccgg ggacctcgag ggcatcctcc
     4741 ggccagtcga acacgtgcag atagagcttg ccgggcgcgg tcgtgcagcg gcc
//
LOCUS       DAOPBQ010000267         4791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14851_length_4791_cov_3.914274, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000267 DAOPBQ010000000
VERSION     DAOPBQ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14851_length_4791_cov_3.914274"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PKW60_09285"
     CDS             <1..269
                     /locus_tag="PKW60_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99664.1"
                     /translation="SSVAFGLRLKFPCFAQRAVNAFVKRFLAGKHGEQKPGVIGGKAL
                     ESAGRTMATGAHCRAAQATNGSSVSTAFLPEISWKRAKLRAGES"
     gene            complement(331..1551)
                     /locus_tag="PKW60_09290"
     CDS             complement(331..1551)
                     /locus_tag="PKW60_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99665.1"
                     /translation="MSIKSLAERALEQGQGILRLTPTWVPRVFCVPGRRIKLHPDDYY
                     ALGGNRGGIDERWFSSTTPADNGPLTSPNEGLSFVVFEDSAEEKLFLLRDAVDELKGA
                     LIGDRIWNEYGRWPMYSKFFDNKGALPHHIHHDDEHAGKVGQLGKPEAYFFPTQLNNH
                     GGDFPYTFFGLQPGVSKDEVRQALKDFVKGDNKLTNLSAAYRLEPGTGWDVPPGVLHA
                     PGSLCTYEPQKASDVFAMYQSLTGDQIVSEDLLWKDTPGEKKGDFDYLVDVIDWDLNV
                     DPNFAQKHFMRPKPVRSVEEMSAEGYQENWICYKSTAFSAKELTVLPGKSAEIKDAGA
                     YGLIVLQGHGTMGAWDIESPALIRYGQLTYDEFFVSEAAAVQGIRITNPSKSDPLVML
                     KHFGPDNPDLVVEP"
     gene            complement(1573..2712)
                     /locus_tag="PKW60_09295"
     CDS             complement(1573..2712)
                     /locus_tag="PKW60_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99666.1"
                     /translation="MRKKLNIGLVGYGFMGRAHTNAYRQAPKFFDLPCDLVLKAACAR
                     DRDAIQAFADNWGYESIETDWRDLVKRDDIDAIDICAPNNVHHDIAIAAAEAGKMVLC
                     EKPLAMNAEEGREMVEAVEKAGVPNMIWYNYRRVPAVTLAKQLIDQGRLGRIFHYRAV
                     FLQDWTISADLPQGGASLWRLDAAAAGSGVTGDLLAHCIDTALWLNGPINNVSAMTET
                     FVKERQHNLTGKVEPVKIDDACAFLARFDNGSLATFESTRYARGHKALYTFEINGEHA
                     SIQWDLHDLHRLNYFDHRDDSVVRGWRSIHVTDGDMPYMDRWWVPGLQIGFEHSFIHG
                     VADFFRGIESGNPAHPTFRDALETQYVCDAVLKSAQTQAWQPVHA"
     gene            complement(2821..3717)
                     /locus_tag="PKW60_09300"
     CDS             complement(2821..3717)
                     /locus_tag="PKW60_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPJ99667.1"
                     /translation="MKRISIGTWAYSIGPYADNPVPWEEILVKLSELGFDGVELGGFG
                     IHPNPNNHPTKEDRDRCKRQVADAGLEFSALAADLWSQHLLDTEDNSAYLDEFRKNLD
                     FCVDLGIPGIRVDSVQPPTIFEQVEPDVARKRAVTAWKQCAREAADKGVYMTWEFEPG
                     FAFNKPSEIIRIVDEVNNDNFGVQFDTCHAHMVAAVGARQPGEKEILPGGAVELARKL
                     RGKINHIHLIDSDGTLHDDETSTHAPFGDGILDFGALLPELNAAGVPHDWWTIDLCFW
                     PDAWAVTARCKAGIDELNRKYG"
     gene            complement(3951..>4791)
                     /locus_tag="PKW60_09305"
     CDS             complement(3951..>4791)
                     /locus_tag="PKW60_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPJ99668.1"
                     /translation="DQIKFQPSTKAPYLASGLERGCIIDTIDHPVNDRMYLEHEFQRI
                     STLPGEDNRLSELEVILHWEDPGSDGFYDDLGNPERQPHLVQQLPWEEDPGRVHSAGQ
                     EFTWHNMKYDDIRRAAGRLSWQDQAYTLYGEPLKMRYTGLYADAKYRLKVMYTGRFAP
                     TMTCTADGLYPVHGPVFDPSYTPLEFDVPHEATSDGVLELQWDLVNGRGCQVAEVWLM
                     VSEFIGIPGDVDHNGKVNAVDVQLVINAALNIKVPFNCDINGNGKVDAVDVQLVINAA
                     LTS"
BASE COUNT          885 a         1352 c         1538 g         1016 t
ORIGIN      
        1 aatcgtcagt cgcgtttggc ctccggttga aattcccgtg cttcgcgcag cgggcggtaa
       61 atgcgttcgt gaaacgcttt ctcgcgggta agcacggcga gcagaaaccg ggtgttatcg
      121 gcgggaaagc cctcgaatcc gcagggagaa ccatggcgac cggggcgcac tgcagggcgg
      181 cgcaggcaac gaacggcagc agcgtgagca cggctttcct ccctgaaatc tcctggaaac
      241 gcgcaaaact cagagccgga gagagttgag atacgaaaac gcgccgcgcc tcagagtgag
      301 acgcggcgcg ttggcgtgta gcgctgagta ttacggctcg accacgaggt caggattgtc
      361 gggcccgaaa tgtttgagca tgaccagcgg atcggatttg ctcgggttgg tgatgcggat
      421 gccctgcact gcggcggcct ccgatacgaa gaactcatca taggtgagct gtccatagcg
      481 gatcagggcc ggggattcga tgtcccaggc gcccatggtc ccgtgaccct gaaggacaat
      541 cagtccgtag gctccggcat ctttgatctc cgcggacttg ccggggagca cggtgagctc
      601 tttggcgctg aacgcggtgg atttgtagca gatccagttt tcttggtagc cctcggctga
      661 catttcctcg acggagcgga cgggtttggg gcgcatgaaa tgtttctgcg cgaagttggg
      721 atcgacgttc agatcccagt cgatcacgtc gaccaggtag tcgaaatcgc ccttcttttc
      781 gccgggagtg tccttccaca ggaggtcttc ggacacgatc tggtcgccgg tgagtgactg
      841 gtacatggcg aagacgtcgg aggccttctg aggttcatag gtgcagaggc tgccgggggc
      901 atggagaaca cccggaggta catcccatcc cgtgccgggt tcaaggcggt acgcggcgga
      961 caagttggtg agcttgttat cgcctttgac gaaatccttg agggcctggc gcacttcgtc
     1021 cttcgacacg ccgggctgaa gcccgaaaaa ggtgtagggg aagtcgccgc cgtggttatt
     1081 cagctgcgtg gggaagaagt aggcttcagg ttttccaagc tggccgacct tcccggcgtg
     1141 ttcgtcgtcg tgatggatgt gatgggggag ggcgcccttg ttgtcgaaga atttggaata
     1201 catgggccag cgaccgtact cattccagat ccggtcgccg atgagagcgc ctttcaactc
     1261 atccacggca tcgcggagga gaaacagctt ttcttccgcg ctgtcttcaa atacgacgaa
     1321 gctgaggcct tcattggggc tggtgagggg accgttatcc gcgggggttg ttgaagagaa
     1381 ccaccgctcg tcgatcccgc cccggttccc gccgagggcg tagtaatcgt ccgggtggag
     1441 cttgatgcgc cggcccggca cgcaaaagac ccggggcacc caggtaggcg tgaggcggag
     1501 gatgccctgg ccctgttcca aggcccgttc agccaaagac tttatgctca tcgtggtgtc
     1561 ctttccattg cgttacgcgt gcacgggttg ccatgcctgg gtctgagccg atttcagcac
     1621 tgcgtcgcac acgtactggg tttccaatgc gtcgcggaag gtaggatggg ccggattgcc
     1681 ggattcgatg ccccggaaga agtccgctac cccgtggata aaggagtgct cgaacccgat
     1741 ctgcagtccc ggaacccacc agcggtccat gtagggcatg tcgccgtccg tgacatggat
     1801 cgaccgccag ccgcgaacaa cggaatcgtc gcggtggtcg aaatagttga gccggtgcag
     1861 gtcatgcaga tcccattgga tcgacgcatg ttctccattg atctcgaagg tgtaaagcgc
     1921 tttgtggccg cgggcatagc gcgtggactc aaaggtcgcg agggaaccgt tgtcgaagcg
     1981 ggcgaggaac gcgcaggcgt cgtcgatttt taccggctca accttgcccg tgaggttgtg
     2041 ctgccgctcc ttcacgaagg tctcggtcat ggcggagacg ttgttgatgg ggccgttcag
     2101 ccacagcgcg gtgtcgatgc aatgggcgag gaggtcgccg gtcacgccac tgccggccgc
     2161 agcggcgtcc agccgccaga gagaggcgcc gccctgcggc agatcggcgc tgatggtcca
     2221 gtcctgcaga aatacggcgc ggtaatggaa gatgcggccg aggcgcccct ggtcaatcag
     2281 ctgtttggcc agagtcaccg cgggtacccg gcggtagttg taccaaatca tgttcggcac
     2341 gcccgctttt tcgacggcct cgaccatctc gcggccttcc tcggcattca ttgccagagg
     2401 tttttcgcac aagaccatct tgccggcctc ggcggcggcg atggcgatgt cgtgatggac
     2461 attattgggt gcgcagatgt cgatggcgtc gatatcgtca cgtttgacga ggtcgcgcca
     2521 gtcggtctcg atgctttcgt agccccagtt gtcggcgaat gcctgaatgg cgtcgcgatc
     2581 gcgcgcacag gccgccttca ggacgaggtc acaggggagg tcgaagaatt tcggcgcctg
     2641 ccggtaggca ttggtatggg cgcggcccat gaatccgtaa cccaccaatc caatattgag
     2701 ttttttccgc atggttcatc tccaacttcc caccggcagc cgcttcggct cccggtggag
     2761 ggccaatgcc atctcaattc gcaaaaaaag cccggccgtt tcgggccggg cgggattcgg
     2821 tcatccgtat ttccggttca attcatcgat accggccttg cagcgcgcgg tcacggccca
     2881 ggcatccggc cagaagcaga gatcgatggt ccaccagtcg tgcgggacac ccgccgcatt
     2941 caactccggc agcagagcac cgaaatcgag aatcccgtcg ccgaagggcg cgtgggtact
     3001 ggtctcgtca tcgtgcaggg ttccgtcgga atcgatgagg tggatgtggt tgatttttcc
     3061 gcgaagtttc cgggcgagtt cgacggcgcc gcccgggagt atttctttct cgcccggctg
     3121 gcgcgcaccg accgcggcga ccatatgcgc gtggcaggtg tcgaactgga cgccgaagtt
     3181 gtcgttattg acttcatcca cgatgcggat gatctcggat ggtttgttga acgcgaatcc
     3241 gggctcgaat tcccacgtca tatagacgcc tttgtcggcg gcctcgcgcg cacactgctt
     3301 ccaggcggtt acggcgcgtt tgcgggcgac gtcgggctcg acctgttcga agatcgtcgg
     3361 cggctgcacc gaatcgacgc gaatgcccgg gatgccgagg tcaacgcaga aatccaggtt
     3421 tttgcggaac tcgtccaggt aggcggaatt gtcttcggtg tcgagaagat gttgcgacca
     3481 gagatcggcc gccagcgcgg agaattccag gccggcgtct gccacctgtc gtttgcaccg
     3541 gtcgcggtct tctttggtgg gatggttgtt gggattgggg tggattccga atcccccgag
     3601 ctcgacgccg tcgaaaccca attcgctgag tttgacgagg atttcttccc agggtacggg
     3661 attgtccgca tagggaccga tagaatacgc ccacgtgcca atcgagattc tcttcatgat
     3721 gctccccatc ctttctagat cttctggttg ccggagttca gacctgttga gtgcaggctc
     3781 cggacggtct ggccgcagcc attcctgacc aagatgcctt agtatgccac gaaacgcggc
     3841 cattctccac gggccgccga atcgggaaac ggcagccgcc gcacttcgaa cagggtggca
     3901 tgcaggctaa caggcgccgg cggcttcgtc aaggctggca cagctgcctg tcacgatgtg
     3961 agtgcggcat taatcacaag ctgcacatcg accgcgtcga ccttgccatt gccgttgata
     4021 tcgcagttga aggggacctt gatgttgagc gcggcattga tgacaagctg cacatccacc
     4081 gcgttgactt ttccgttatg gtccacatcg cccgggatgc cgatgaattc actgaccatg
     4141 agccacactt ctgccacctg gcagccacgg ccgttcacca aatcccactg gagttcgagg
     4201 acgccatcgc tcgtggcttc gtgcggcacg tcaaattcca gaggagtgta gctggggtca
     4261 aaaacgggcc catgcacggg atacaggccg tcggctgtgc aggtcatggt gggagcgaat
     4321 cggccggtgt acatgacttt caagcggtat ttggcgtcgg cgtacagccc ggtataacgc
     4381 atcttgagag gctctccgta cagcgtgtac gcctggtctt gccaggaaag gcggccggcc
     4441 gctctccgga tgtcgtcgta tttcatgtta tgccaggtaa attcctggcc ggcagagtgc
     4501 actcgtccgg gatcttcctc ccatggcagt tgttggacca ggtgcggctg gcgctccgga
     4561 ttacccaggt cgtcgtagaa gccgtcgctt cccggatctt cccagtggag gatcacctcg
     4621 agctcgctga gccggttatc ctcgccggga agcgtgctga tgcgctggaa ttcatgctcg
     4681 agatacatgc ggtcgttaac aggatgatcg atggtgtcga tgatgcaacc gcgttcgagg
     4741 ccgcttgcca ggtagggcgc cttcgtgctg ggctggaact tgatttgatc c
//
LOCUS       DAOPBQ010000268         4770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_14917_length_4770_cov_4.049841, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000268 DAOPBQ010000000
VERSION     DAOPBQ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14917_length_4770_cov_4.049841"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..783)
                     /locus_tag="PKW60_09310"
     CDS             complement(130..783)
                     /locus_tag="PKW60_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99669.1"
                     /translation="MALPLAGLAIAAGALFWLIHTPAPMGTVSVVGLTGIAQPKVSEG
                     RAGPAPASGPGPVKAETTVRQTDPTTIYRLLGTMGTGQAFGFESRLGRWTLGVELLAD
                     LTWLTGIGFSYHEAFSCRFSGCAFLDYPHFPLLSEWLVGGIAGALTAAVIYGLLFTSI
                     LRSGWRGWTSGSSAVAVAVLPYALLSGDTLFSIPQFIIVCLLAQSQAETGSVSEEKE"
     gene            complement(972..1472)
                     /locus_tag="PKW60_09315"
     CDS             complement(972..1472)
                     /locus_tag="PKW60_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99670.1"
                     /translation="MALAAPFAAATLPYPWLLLSLVVCIPFAWHLARRGLSFPGAGCV
                     PTVIWCVLILGLLHLWGLRSGPTPFWPRVVVDMGIVGAGVAVFLFGRVEKGSGTGMME
                     GFFAALIPFVAVTAAIGLVKAALLERGVLLGLLLSLYPDQYPPGSSLRGDTGLFGLSC
                     SLPDWG"
     gene            complement(1690..4350)
                     /locus_tag="PKW60_09320"
     CDS             complement(1690..4350)
                     /locus_tag="PKW60_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99671.1"
                     /translation="MLDVSVIAWVWMLSAMLVSSGAGLALSRLVTWTDPARQAGIPRA
                     FGLAIAPFLLGLMAVVALGVFRGASHAFHLGVVFAGLLALCATACFTRPVGRPVSRET
                     SQPMGLWDWIFGGILAVWVLALLVNAALLPLLQNDSLEYAIVGRLLFESRDLLSYPAI
                     HPEQSSSGFYGPWTHPPLYVALIYLMYVFQGHAEMPGLMRTIAPWCALAATGLVFALG
                     NLTNRLTGILSSLFFLTVPLFFLGAGSSLLDALPVLGITIVMAGIIGVQGPPLRRSLM
                     RGLVLGAALWAHSVAVIFLPMAVLAIALNDGWRNIRGFMVQAATVVGAATLVAAWPYC
                     RNLTLFGSLISDNPAVFAMPELAWEEYFRLTRGIDSWPEKIQYGILKGWFALEAYSLL
                     FWLMLLGAAIYMRRLVSEVNAPRIRAGDLSSVPDPWNLTALAVLICFMGGVILSTLMG
                     IDLMIRNERYWLITIPFAAVLAGGFISDRPGGIKNLDGAGVVAKPVYRFKVVAPLFAL
                     LFLLQAIITIAVYCWIPYLRISPALVVETQSQSPLQKFLSDRGVSDVLLGSVDGRIIL
                     EENAAQVGEKTVPSGISALGKSIHDKLFLWPAMNAVLFLRESVPISSTVLSLRPADMF
                     YSGRKMVSYLDPRLLPVYREENALQAWQMLRELGIDYLHLPDYYIPTMYNSVLMELCA
                     RPDLAEMVFSADGTQIYKLLQREVRLSEKAFDLTPGKMPWTTVSKMTGGRKALVGLHS
                     REATIEMGGATDKRAGLWLFRRDWSTEHISGIGFLDKPLALKTHVPVRGDDEYRLEID
                     LEGHSYARVSLAILDEKGNCLQDKYFFSGCQVRIGEIVLGPAHPENRFVRRFVTPPAA
                     AYIRVGVEHIGGSELHIRQARLIALAGNHR"
     gene            complement(4410..>4770)
                     /locus_tag="PKW60_09325"
     CDS             complement(4410..>4770)
                     /locus_tag="PKW60_09325"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPJ99672.1"
                     /translation="LCVAAAFWVLTTYFVPGKDLHLGLVPLGILLAGLTAISVFIPFD
                     NLMLVSGHPGLQTLQHLTLVLSNIVLNVSLIPLLGIMGAAVGTALSYICGIGALIFLA
                     RRMLGWNLLTNRIRVPS"
BASE COUNT         1075 a         1438 c         1456 g          801 t
ORIGIN      
        1 gacggaccag ggccgcgaca gccagcagca gcggcgagag gacgatcagc gccacgacgc
       61 ttgcaacggc atcgaagatg cgttttgcgg taccgttcat gggttgggtc tggcggggtg
      121 caaggccgct cactcctttt cctccgatac gctgccggtc tcggcctgac tctgcgccag
      181 caggcagacg atgatgaatt ggggaatgga aaagagcgtg tcccccgaga gcagcgcgta
      241 gggaaggacg gcgaccgcga cggcgctgga gccgcttgtc catcccctcc accccgaacg
      301 caggatggac gtgaagagaa gtccgtagat gaccgcggcc gtcagggccc cggcaatgcc
      361 tcccacaagc cactccgaca ggagcgggaa atgcggataa tccagaaagg cacaccctga
      421 aaagcgacag gagaaggcct cgtgatacga aaacccgatt cccgtcagcc aggttagatc
      481 ggcaaggagc tcgacgccca gagtccatct gcccaagcgg gactcgaaac cgaacgcctg
      541 gccggtcccc atggtgccga gcagccggta gatcgtcgtc ggatcggtct gccggactgt
      601 ggtctctgcc ttcacgggcc cgggaccgga tgccggtgcc gggccggctc tgccttccga
      661 cactttgggc tgcgctattc cggtgagtcc gacgacgctg acggttccca tcggcgccgg
      721 tgtgtgtatc agccagaaca gcgcccccgc ggcaatcgca agtccagcca gggggagcgc
      781 catgcgaaag gcgatttccc gcttcctgct tccccggcaa gagacggcaa gccatacaca
      841 gggaatcagg aaggagagca ccaggaatcg tctcgactcc gtgagcatcc ccgccgatac
      901 gatgacggcg agcgccagaa cggcacaggt cgcgcgcgcc caagagctgc gaccgctcag
      961 gcgcgtaatc gctatcccca atccggcaac gagcatgaaa ggccgaaaag gccggtgtcc
     1021 cccctcaggc tcgagcctgg aggatactgg tcggggtaga gcgacaggag caagcccagc
     1081 aggacgcccc tctccagcaa cgctgccttg acgagaccga tggctgccgt cacggcgaca
     1141 aaggggatca aggcggcaaa gaacccctcc atcatccccg tgccggagcc cttttccacc
     1201 cgtccgaaga gaaagacagc gacgcccgct ccgacaatcc ccatgtctac gacaacccgc
     1261 ggccagaaag gggtcggtcc cgaccgcagt ccccacaggt gaagaaggcc gaggatcaaa
     1321 acgcaccaga tgactgtcgg cacgcatccg gccccgggga acgagaggcc tcgccgggca
     1381 agatgccatg cgaagggtat gcagacgacg agagacagca gcagccaggg gtatggtagc
     1441 gtcgccgccg cgaagggggc tgccagggcc atgccggcgg ccctgggacg cctgttcggg
     1501 ccgggctgcg gaactctatg tgccttgcca cctttcatcc tgcctgtttc tcccgtgtca
     1561 gggttgtgcc gggtctgtgg ctgcgagaat cggggtcagc atctcctcag tgctgagaat
     1621 cttggaacga ctattcgccc gacttcgatt cggagttcga cgaccagggg agtgcacaca
     1681 tgcaactgat tatcggtggt ttccagcaag agctatgagt ctcgcctggc ggatatgcag
     1741 ttctgaaccc ccaatgtgct cgacaccgac tcggatatag gcagctgccg gcggcgtcac
     1801 gaaacggcgg acaaagcgat tctcggggtg ggcgggtccc aggacaattt ctccaatccg
     1861 cacttggcag ccggaaaaga aatacttgtc ctgcaggcag ttaccctttt cgtccaggat
     1921 tgcgagcgag accctcgcat agctatgtcc ttccagatcg atttccagcc tgtactcgtc
     1981 gtcgcctcgt acgggcacat gcgttttcag ggcaagcggt ttgtccaaaa aaccgatccc
     2041 gctgatgtgc tccgttgacc aatcgcgcct aaacaaccat agacctgctc ttttatcggt
     2101 ggcgccaccc atttcgatcg tggcttcccg cgagtgcaag ccgaccaatg ctttccggcc
     2161 accggtcatt tttgacactg ttgtccacgg cattttcccg ggagtcaagt cgaatgcctt
     2221 ttcactcaaa cgaacctcac gttggaggag cttgtaaatt tgcgtgccgt ccgcggaaaa
     2281 gaccatttct gccaaatcgg ggcgcgcaca gagctccatg agcacggagt tgtacatggt
     2341 tggaatatag tagtcgggaa ggtgaagata gtcgatgccc aattcccgca acatctgcca
     2401 cgcctgtaga gcgttctctt ccctgtaaac gggaagaaga cgcgggtcca ggtaactgac
     2461 catctttctg cccgagtaga acatatccgc cggcctcagc gacaggacag tcgaagagat
     2521 gggcacagac tcccggagaa acaaaacagc gttcatagcc ggccatagga atagtttgtc
     2581 gtggatgctc tttcccaggg cgcttatccc ggatggtacg gttttctcgc caacctgtgc
     2641 tgcattctcc tcaaggatga tcctgccgtc gacggaaccg agcagcacat cggagacccc
     2701 cctgtcgctg aggaattttt gcagcggcga ctgtgattgg gtttctacga ccagggcagg
     2761 ggatatcctt agataaggta tccaacagta tactgcgatt gtgatgattg cttgcaataa
     2821 aaacagcagc gcaaacaatg gcgcgacaac tttgaacctg tatacgggtt tcgcgaccac
     2881 tcccgctcca tccagattct ttatgccacc gggcctatcg gagatgaacc cgccggccag
     2941 aaccgccgcg aaggggatcg tgatcagcca atagcgctcg ttgcggatca tgagatcgat
     3001 tcccataagc gtcgagagga tgactccgcc catgaaacat atcagtactg ccaacgcggt
     3061 gagattccac gggtccggca cgctgctcaa atctcccgca cggatacggg gtgcattgac
     3121 ctcggataca agccttctca tgtatatcgc ggcacccagg agcatgagcc agaacagcag
     3181 cgagtaggcc tccagggcaa accaaccctt tagaattcca tattgaattt tctccggcca
     3241 cgagtcgatg ccccgggtca gacggaaata ctcctcccag gcaagctccg gcatggcaaa
     3301 cacagccggg ttgtcgctga tgagggagcc gaacagcgtg agatttctac aataaggcca
     3361 tgcggcgacc agcgttgcag caccgacgac ggttgctgcc tgaaccatga acccgcggat
     3421 gtttctccaa ccatcgttca gtgcgattgc cagcacggcc attggcaaaa aaataacggc
     3481 tacggaatgc gcccacagcg cggccccgag cacgagtccc cgcatcaggc ttcttcggag
     3541 cggcggtccc tggactccga tgatgccggc catcactatc gtaatgccca aaacgggtaa
     3601 cgcatcaagc agggaggaac cggctcctaa aaaaaacagc gggacggtga ggaaaaacaa
     3661 agaggagagg atccctgtca gccggttggt cagattgccc aatgcgaaca ccagacccgt
     3721 ggccgccagc gcacaccagg gggcgatggt ccgcatcaac ccgggcatct ccgcgtgccc
     3781 ctgaaaaaca tacatcaggt atataagggc cacatagagg ggaggatgcg tccacgggcc
     3841 gtaaaaaccg gaactgctct gctctggatg aatcgcaggg taggacagca ggtcgcggga
     3901 ctcgaagagc aatcgtccga cgatggcata ttccagggaa tcgttctgga gcaggggtag
     3961 aagagcggcg ttgaccagca aggccagtac ccacaccgcc aggattcccc cgaagatcca
     4021 gtcccagagg cccatcggtt gggacgtttc gcgcgacacc ggccggccga cgggacgggt
     4081 gaagcaggcg gtggcgcaca gcgcgagcag tcctgcgaaa accacgccga gatgaaaggc
     4141 atgactggcc ccgcgaaaga cgcccagggc gacgaccgcc atcaggccca gcaggaaggg
     4201 ggcgattgca aggccgaagg cgcgtgggat gcccgcctgc cgggcgggat ccgtccaggt
     4261 cacgaggcgc gagagcgcca gaccggcccc gctcgaaacg agcatggcgg aaagcatcca
     4321 gacccaggcg atgacggaga cgtcaagcat gcgaactcac ggtctggggc cctccgttcg
     4381 gtgattgccc caaaggggat tctctcgggt catgacggca ctcgaatccg gttggtcagc
     4441 aggttccagc ccagcatcct gcgtgcaagg aaaatgaggg ccccgatccc gcagatgtag
     4501 ctgagggccg tcccgacggc ggcccccatg atccccagca gggggatcag ggagacgttc
     4561 agcacgatgt tgctcaggac cagcgtgagg tgctgcaggg tctgcagccc ggggtggccg
     4621 ctgacgagca tcaggttgtc gaaggggatg aagaccgaga tcgcggtcag tccggctagg
     4681 aggatgccca gcgggacgag gcccaggtgg aggtccttcc cgggcacgaa gtaggtcgtg
     4741 aggacccaga acgccgccgc gacgcagagg
//
LOCUS       DAOPBQ010000269         4741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15028_length_4741_cov_3.746692, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000269 DAOPBQ010000000
VERSION     DAOPBQ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15028_length_4741_cov_3.746692"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PKW60_09330"
     CDS             complement(<1..1145)
                     /locus_tag="PKW60_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPJ99673.1"
                     /translation="MQHWRLAIIVGAVAGLMGPAYAQIREFGLSGAGEQTANEEVSGS
                     AAEWAIESTVAGMSLRTRVSQLLMVTLQGRLAPDATERQLLENYTPGAVLISSLTRPR
                     DAVDYIAALQRTPVRGQHGVPMLIGADLADLPVVFGMQKEDYFAPLPTMMAVAAADDP
                     VATRGLAALYAEHLALMGFSFQMGPCLALGPDLPYAKGGLQCLGANPAFVGETAEIFL
                     QSFAERDLAVMFTGFPGGQWNQTGTAPPMLFTPASLLPTRDLLPFQRAVLGGARLILV
                     GNIVVPHLDKQNAAASISPAVMTGLLREKLLYPGIIVAGPIDGLGVGKGATQGDAALA
                     ALKAGADMLFWRQPGIHVMKAAEAVVQAVENGHYPESAINASVTRVL"
     gene            1672..2193
                     /locus_tag="PKW60_09335"
     CDS             1672..2193
                     /locus_tag="PKW60_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPJ99674.1"
                     /translation="MLCERCHKRLATLRYSEVIDGKAIARSICQNCLNEIQGDASTGF
                     EMSGAAPPPRHDLLQRVATDRLARHIVCSACGLQLAEGLQRGILGCPVCYDSFADHLP
                     NVLRGIQPGLRHRGKMPSRDTSREQLHMQLQTRRALLRSALKTENYEDAARLRDEIKG
                     LEVALNSGAPHKG"
     gene            2201..3244
                     /locus_tag="PKW60_09340"
     CDS             2201..3244
                     /locus_tag="PKW60_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99675.1"
                     /translation="MLETLLEKKARWLSGEGPEADLVIESGGTLVRNLADLPFPGLAS
                     DDELHNVLDRVLGAMEGHALFRSGQFFMVEHLEPRVVRFLVERRLITPDMERAHGPRG
                     VWLSDDQAFSVMINGRDHVRLQVRSAGFNLDEVWTQLSHADDILGSTLDFAFHDKFGY
                     LSASVNEVGTGFGLFAIAHLAGLAMNNRMLAESERARESGHRIEGLFSSVSEGLGELF
                     TVANTATLGRSEEEIQFNLRHLVTGLLESERHAREQLHTEGARAVEDRVGRAAGIARG
                     AHLLEFNEALSLLSSLRLGRTMEYIKKYTYKQLNKTLYAAQPAHLAMRAGQDCDELAL
                     SMMRADLFRSTFS"
     gene            3311..>4741
                     /locus_tag="PKW60_09345"
     CDS             3311..>4741
                     /locus_tag="PKW60_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPJ99676.1"
                     /translation="MWDRFTERAKHVISTAREEATRLGSEYVRTEHILLGLCREPDGI
                     AARALENLGVDIESLAMEIEQQVQRGNAVPSSDEIAFTPRAKKVLELSVEEARRFNHS
                     YIGTEHILLGLVKEGEGIAAKVLQDLKIDLDKIQAEVIRLLGDQGKTGTQASSGKKSQ
                     TPALDTFGRDLTALAQEGKLDPVIGREGEIERVIQVLSRRTKNNPVLIGEAGVGKTAI
                     VEGLAQAIIKGDVPDLLLGRRVLTLDLAGVVAGTKYRGQFEERLKSVMKEIRRADNII
                     LFIDELHTIVGAGAAEGAVDAANMLKPALARGELQCIGATTMDEYRKHIEKDAALARR
                     FQTILVEAPTVDQTIEIIHGLRDKYEAHHRVKFSDDAIVAAAKLSNQYITDRFLPDKA
                     VDVIDEAGSRARLQITTRPPALKEIEQEIDRVTQEKETAIRRQEYEKAAGLRDRERTL
                     IALLEEKKREWEKKRDSAETIVTTDDI"
BASE COUNT          966 a         1481 c         1511 g          783 t
ORIGIN      
        1 tgaagaacgc gcgtcacgga cgcgttgatg gcggattcgg gatagtggcc gttttccacg
       61 gcttgcacga ccgcttccgc agccttcatc acgtgaatgc cgggctgcct ccagaacagc
      121 atgtcggcgc ccgccttgag agcagccagg gctgcgtcgc cctgtgtggc gcctttccct
      181 acccccagcc cgtcgatggg cccggctacg atgattccag gatacagcag cttttcacgc
      241 agcagcccgg tcatcactgc cggggaaatg ctggctgccg cattttgttt atcgagatgc
      301 ggcacgacaa tgttgccgac caggatcaac cgcgcacctc ccaataccgc ccgttggaac
      361 gggagcaggt cacgcgtggg caggagcgac gccggcgtga acagcatcgg gggcgccgta
      421 cccgtctgat tccactggcc gccggggaag cccgtaaaca tgaccgccag gtcgcgctcg
      481 gcgaacgact ggaggaaaat ctccgccgtt tcccccacga acgccggatt tgcccccaga
      541 cactgaagcc cacctttcgc atagggaagg tctgggccga gagcgaggca tggccccatc
      601 tggaacgaga agcccatgag ggcgagatgt tccgcataca gcgcggccag tccgcgggtc
      661 gcgaccgggt cgtccgccgc ggccaccgcc atcatcgtgg gaaggggggc aaaatagtct
      721 tccttctgca tgccgaaaac gaccgggagg tccgccaggt ctgcgccgat cagcatcggc
      781 acaccatgct gcccgcgcac cggggtgcgc tgcagcgccg cgatataatc cacggcgtct
      841 cgcggccgtg tgaggctgga aatgagtacg gcgcccgggg tgtaattctc gagcagctgg
      901 cgctcggtgg cgtcgggtgc gagccggccc tgcaaggtca ccatcagcag ctgcgatacg
      961 cgggtgcgca ggctcatacc cgcgacagtg gactcgatgg cccattcggc agccgatccg
     1021 ctgacctctt cgttcgccgt ctgttcgccc gctccggaca atccgaattc acgaatctgg
     1081 gcgtacgccg ggcccatcag acccgccacc gcacccacaa taatcgcaag tctccagtgt
     1141 tgcatcgcgc tgctcctcga cgggatttta gcagcgcctc tcgccctgca caacgtcaac
     1201 acgccgacat ggcatgggca gcgaggcagt ggacgctgcc ggaggaaacc agtatcgggg
     1261 cgggaactcg ccgcggcgcc tgaacccgcc ttgcgccccg gaccctgtcc gggccgccag
     1321 gcggggcagg aacgaggccc gtacggccgc tgtagaaggc gcccggtacg ttcgagcgcg
     1381 taataaacat tcccgatgcc ctggcccttc aattcaagcg ggaaaaccgc atttacatgc
     1441 gattgcctga ctttgccgct gattgggtgc agtgcccggc ctgggcggaa cagggcggct
     1501 ccgcggcgtg ttcattcgga aagagaagcc cgggaatgca tttccggccg ggcgctgccc
     1561 ttgggccgtc ctttcagggc tggacagcca gcgcgccgcg tgggttccag tcgatgtgcg
     1621 tggacgcccc ccacgcggga atgctatact tgacttgagg gctgaagaac tatgttatgc
     1681 gaacgttgtc acaagcggct ggcgaccctg agatatagcg aagtcattga tggcaaagcc
     1741 attgccagaa gcatctgcca gaattgcctg aatgagattc aaggcgatgc cagcacgggc
     1801 ttcgaaatgt ccggcgccgc gcctccgcca cgccacgacc tgctgcagcg ggttgccacg
     1861 gaccgtctgg cgcggcacat cgtgtgttcc gcatgcggcc ttcaactggc ggaagggctg
     1921 cagcggggga tcctgggctg ccccgtatgc tacgacagct ttgcggacca tctcccgaat
     1981 gtgctccgcg gcatccagcc ggggctgcgc catcgcggca agatgccgtc gcgggatacc
     2041 tcgcgcgaac agctgcacat gcagcttcaa accaggcggg cgttgctgcg cagcgccctg
     2101 aaaaccgaaa attacgagga cgccgcccgc ttgcgcgacg aaatcaaggg tttggaggtc
     2161 gcgctcaatt ccggggcgcc tcataagggt tgaacacctg atgctggaga cgttgctcga
     2221 aaagaaggcc cgatggctgt ccggcgaagg acccgaggca gacctcgtca ttgaatcggg
     2281 cggcacgctt gtccggaatc tggccgatct gccgtttccc ggcctggcct ccgatgacga
     2341 gctgcacaac gtgctggatc gtgtgcttgg cgccatggaa ggccacgccc tgttccggtc
     2401 ggggcagttt ttcatggtcg aacacctgga accgcgcgta gtgcggtttc tggtggaacg
     2461 gcggctcatc actccggaca tggaacgtgc tcacggcccg cgcggggtct ggttgagcga
     2521 cgaccaggca ttcagcgtaa tgattaatgg gcgggatcat gtgcggctgc aagtccgctc
     2581 ggcggggttt aacctcgatg aggtgtggac ccagctcagc cacgccgacg acatcctggg
     2641 cagcacgctg gatttcgcct tccacgacaa attcgggtac ctcagcgcgt cagtgaacga
     2701 agtaggcacg gggttcggcc tgtttgccat cgcccatctt gcgggtctcg ccatgaacaa
     2761 ccggatgctg gccgaaagcg aaagagcgcg cgaaagcggt caccgtattg agggcctgtt
     2821 cagctcggtc agtgaaggat tgggtgagct cttcaccgta gccaatacgg ccacgctggg
     2881 gcgctccgaa gaggaaattc agttcaacct gcggcacctg gtcactggcc tgctcgaaag
     2941 cgagcgccac gcacgggaac agcttcatac ggaaggcgct cgcgccgtcg aggaccgcgt
     3001 cgggagggca gcgggcattg cccgtggcgc ccatctgctg gaattcaatg aagccctgag
     3061 cctgttgtct tcgctgcggc ttggccgcac gatggaatac attaagaaat atacgtataa
     3121 acaactgaat aagacgctgt atgccgccca gccggcccac ctcgccatgc gggcgggtca
     3181 ggattgcgat gagctggcgc tgagcatgat gcgggcggac cttttccggt ccacgttttc
     3241 ctgagccgcc gactgcgatg cgtgctgccg ggcgatccga aggcacagac agggagtaac
     3301 gactagaacg atgtgggaca ggtttaccga acgcgcaaaa cacgtgatca gcacagcccg
     3361 ggaagaagcc acgcgcctcg gaagcgaata cgtgcgcacc gagcacattc ttctcggcct
     3421 gtgccgcgaa cccgatggga ttgcggcgcg cgccctggag aatctgggcg tcgatattga
     3481 atcgctcgcg atggagatcg agcagcaggt gcagcggggc aacgcggttc cgtcgagcga
     3541 cgagatcgcg ttcacgcccc gggcgaagaa agtcctggag ctgtctgtcg aagaagcgcg
     3601 ccggttcaac cacagctaca tcggcacgga gcacatcctc ctgggcctcg tgaaagaagg
     3661 cgaagggatc gccgccaaag tgctccagga cctcaaaatt gatctcgaca aaatccaggc
     3721 ggaggtgatc cggctgctgg gcgatcaggg caagacgggc acgcaggcct ccagcggcaa
     3781 gaagagccaa acgcccgcgc tagacacgtt cgggcgcgac ctcaccgcgc tggcccagga
     3841 aggcaagctt gacccggtca ttgggcgcga aggcgagatc gagcgggtga ttcaggtgct
     3901 cagccgccgc acaaagaaca atcccgtgct gattggagag gcgggcgtag gcaagaccgc
     3961 catcgtggaa ggcctggcac aagccatcat caagggcgat gtgcccgacc tgctgctggg
     4021 ccgccgggtg ctgacgctcg atctggccgg ggtggtggcc ggcaccaagt accgcggcca
     4081 gttcgaggag cgcctgaaat ccgtcatgaa ggaaatccgg cgcgcggaca acatcatcct
     4141 tttcatcgac gaattgcaca cgattgtcgg ggcgggcgcc gcggaaggcg ccgtcgacgc
     4201 cgccaacatg ctcaagcccg ccttggcgcg cggcgaactg cagtgcatcg gcgccaccac
     4261 gatggacgaa taccgcaaac atatcgagaa ggatgcggct ctggcccgcc gcttccaaac
     4321 gatcctggtc gaagccccca ccgtcgatca gaccatcgag atcatccacg gactgcgcga
     4381 taaatacgag gcgcaccacc gagtcaaatt ctcggacgac gccattgtcg ccgccgccaa
     4441 gctttccaac cagtacatca ccgaccgttt cctgcccgac aaagccgtcg acgtcatcga
     4501 cgaagcgggc agccgcgcgc ggttgcagat cacgactcgg ccgcccgcgc tcaaggaaat
     4561 cgaacaggag atcgaccgcg tcactcagga aaaagagacg gccatccggc gtcaggaata
     4621 tgaaaaagcg gcggggctgc gcgatcgcga acgcacgctg atcgcgctgc tcgaagaaaa
     4681 aaagcgggaa tgggagaaaa aacgcgactc cgccgaaacc atcgtcacca ccgacgacat
     4741 c
//
LOCUS       DAOPBQ010000270         4722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15103_length_4722_cov_3.860081, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000270 DAOPBQ010000000
VERSION     DAOPBQ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15103_length_4722_cov_3.860081"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PKW60_09350"
     CDS             complement(<1..1159)
                     /locus_tag="PKW60_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPJ99677.1"
                     /translation="MKAIIMVAGKSTRTYPLTLTRPKPLLKVVNKPILAWLLDALRPV
                     IDEAILVVGYRGDMVRDAFGAAYNGLPLRYVEQTEQLGTGHAVLQCEAYVDGPFIAMN
                     GDDLYAPEDLQALAGTTNTALAKRVADPRQYGVLELDDRGRVARLVEKPQEPASDLVS
                     VGAYTFTPEVFDVLRKTKPSERGEIEITSAIHELASRGDFHALTMAGYWLPVGYPWDL
                     LAANEQLLKKMTPAIEGSVHPRAELSGPVHVGRDTVIRSGVVIDGPVYIGEGCTVGPN
                     TWLRPGATLYNECRVGQSCEIKNSILFDGCAVPHLSYVGDSIIGERTNFGAGTITANV
                     RHDGGTIKSMVKGEMIDTGRRKLGAIVGDHVHTGIKTSLYPGRKLWPGTCTR"
     gene            complement(1187..1942)
                     /locus_tag="PKW60_09355"
     CDS             complement(1187..1942)
                     /locus_tag="PKW60_09355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPJ99678.1"
                     /translation="MIDFNAKLGHFPYRPVEGLEALLHAMDRHGVEQALISSLHAVFY
                     LNPQDGNDELAAAIAPHRDRFFLSAVLRPNFTGWRDDLRRCVDDYGARAVLLYPQYHC
                     YELLAPEMEDLVAGAHDLGLPICVQCWLEDPRRQFHREIIAPVPPETLAAFIQAWPQA
                     TVVALGLRFNEPDQLGEPFPRNAHFDISNYENMCDLELAMERFPVDRLLFGSNFPLYN
                     YVANVDKLARGNLTGAQRAAIGRENAKRLLGIP"
     gene            complement(1939..2691)
                     /locus_tag="PKW60_09360"
     CDS             complement(1939..2691)
                     /locus_tag="PKW60_09360"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPJ99679.1"
                     /translation="MSLVIDCHAHLRTPGGGDPERLLEAADRAGVDRLILCSLGREWM
                     VFPDAATLEQANADIVEVLERYPDRLMGLVYLSADDVALGLEHLNRYIANGPCVGVKL
                     WVSQLADDPRMDPLFDRIVELDVPVLQHTWFKATGNLEKESTAWHCVNRAELHPGLKM
                     WLAHAGGQWEETARIIAPYPNLCVDISGGEPEAGVVDCLLRHIGPERIFFGSDIPGRS
                     LIVQMSKVLSAEIPDGWKEMILGENILRWVRP"
     gene            2933..3418
                     /locus_tag="PKW60_09365"
     CDS             2933..3418
                     /locus_tag="PKW60_09365"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ99680.1"
                     /translation="MSRSLLLDLKTLAKNLFKHLRYVLNRRESVRCALSGMVSDVKKL
                     RVHVGAQHATERRKVALKYVKQGRTAYNAKNDALAEKLFREAVAADETCALAYAYLGN
                     ALYRQGQTDEAEVSWRRATMIDPSSEGAEKALHSLQHLARKKKAYMDHLDDKLRHGRS
                     S"
     gene            3387..4553
                     /locus_tag="PKW60_09370"
     CDS             3387..4553
                     /locus_tag="PKW60_09370"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPJ99681.1"
                     /translation="MTNCDMDVLPDFSYFMPTEIRFKAGCLNELAVCSRALGQKPFLL
                     TGRHSARATGALDRALAQLPNAVVYDKVEENPTTGQCDAAAQVCREAGCDVVIAIGGG
                     SPMDVAKAVAGLALNEGPCAAFIGSGLFAAGALPIIAVPTTAGTGSEVTPYAVLIDAT
                     EHQKRTIKGRALFPRVALLDPELTAPLPPEVTVYTGLDALSQGMEGLVSRQHTAVGDI
                     LALEVCRLVARWLPRAVHYPHDLEARGGMLLAAMLSGCVIAQSGTTLVHGMGYYYTLH
                     CGLQHGQANGLLLAPVFRHNAAHLPEAVASIAAALGCAGPPEEAGSNITRAIHALFDA
                     CGVSPAARDAGVDESLLAGFAKDCHADPYRFKNQVGELTEHDVLRFYLESWRGA"
BASE COUNT          882 a         1462 c         1502 g          876 t
ORIGIN      
        1 gccgggtgca cgtgcccggc cacagtttcc ggcccgggta cagagaggtc ttgatgcccg
       61 tgtgcacgtg atcgccgacg atggcgccga gctttctccg gcccgtgtcg atcatctcgc
      121 ctttgaccat ggacttgatc gtgccgccgt cgtgccggac attggcggtg atggtgcccg
      181 cgccgaagtt ggtgcgctcg ccgatgatcg agtcgccgac gtagctcagg tgcggcacgg
      241 cacacccgtc gaagaggatc gagttcttga tctcgcagga ctggcccacc cggcattcgt
      301 tgtacagggt cgcgccggga cgcagccagg tgttggggcc gaccgtgcag ccttcgccga
      361 tgtagaccgg cccgtcaatc acaacgccgc tgcgaatgac cgtgtcacgg ccgacatgca
      421 cggggccgct cagttctgcg cggggatgca cgctgccttc aatggccggg gtcattttct
      481 tcaagagctg ctcattggct gccagcaggt cccaggggta gccgacaggc agccaatatc
      541 ccgccatcgt gagcgcatgg aaatctcccc gcgaggccag ttcgtggatg gccgaggtga
      601 tctcgatctc gcccctctcg gacggcttcg tcttacgcag cacgtcgaac acttcgggcg
      661 tgaaggtgta ggcgcccacg ctgaccaggt cggaggccgg ttcctgcggc ttttcgacga
      721 gccgcgccac gcggccccgg tcgtccagtt ccagcacgcc gtactgccgc ggatcggcga
      781 cgcgtttggc cagcgcagta tttgtcgtgc ccgccagtgc ctgcagatcc tcgggagcgt
      841 acagatcatc gccgttcatg gcgataaacg gcccgtcgac atacgcttcg cactggagaa
      901 cggcgtggcc cgtgccaagt tgctcagtct gttcgacgta gcgcagaggc agcccgttgt
      961 acgcggcgcc aaaggcatcg cggaccatgt ccccgcggta tccgacaacg agaatggctt
     1021 cgtcgatgac cgggcgcagc gcgtccagaa gccaggccag aatgggttta ttgacgacct
     1081 tcaacaacgg tttcggccgc gtgagcgtca atggatacgt ccgggtcgat ttgccagcga
     1141 ccatgatgat tgccttcaca acgattctcc tgtccggcgc cgccagttac ggaatgccca
     1201 ggaggcgctt tgcgttttcg cggccgatcg ccgcgcgctg cgctccggta aggttgcccc
     1261 gcgccagttt gtccacgttg gccacgtaat tatagagcgg gaaattgctc ccgaaaagca
     1321 ggcggtccac agggaaacgc tccatggcca gttcgagatc gcacatattt tcgtaattcg
     1381 agatatcgaa atgggcgttt cggggaaagg gctcgccgag ctggtccggt tcgttgaaac
     1441 gaagccccag cgcaaccact gtggcctgag gccatgcctg gataaaggcg gccagggttt
     1501 ctggcggcac cggcgctatg atctcacggt gaaactgacg gcgcgggtct tcgagccagc
     1561 attgcacaca gatcggcaaa ccgaggtcgt gcgcgcccgc gaccaggtct tccatttcag
     1621 gcgccaggag ttcgtagcag tggtattggg ggtagaggag caccgcccgg gcgccgtaat
     1681 cgtcgacgca gcggcggaga tcatcgcgcc agccggtgaa attcggccgc aaaacggcgg
     1741 acagaaaaaa acggtcgcgg tgcggcgcaa tggccgcggc cagctcgtcg ttgccgtcct
     1801 gagggttcag gtaaaacacg gcatgaagcg acgagatcag cgcctgttcg acgccatggc
     1861 gatccatggc atgcagcaac gcctccaacc cctccaccgg gcggtacggg aaatgcccca
     1921 gttttgcgtt gaaatcgatc atggccgcac ccagcgcaag atgttttctc cgagaatcat
     1981 ctctttccaa ccatcgggga tctcagcgga gaggactttg ctcatctgaa caatgaggct
     2041 gcggcccggt atgtcggacc cgaagaagat gcgctcaggg ccaatgtgcc ggagcaggca
     2101 gtccacgact ccggcctccg gctctccgcc ggagatgtcg acgcacaaat tggggtaggg
     2161 cgcaatgatg cgggccgttt cctcccattg gccgccggca tgagccagcc acatcttcag
     2221 gccgggatga agctccgcac gattgacaca atgccacgcg gtcgactcct tttccaggtt
     2281 gccggtggct ttgaaccagg tgtgttgcaa gacaggcaca tcaagctcca cgatccggtc
     2341 aaacagcgga tccatgcggg gatcgtcggc aagctgtgaa acccacagtt ttactcccac
     2401 gcacgggccg ttggcgatgt agcggttcag atgttcgagg ccaagggcca cgtcatccgc
     2461 gctcagatag accaatccca tcaaccggtc gggataacgt tcgagcacct ccacgatgtc
     2521 ggcattggcc tgctcgagcg tcgctgcatc cgggaaaacc atccattcgc gccccaggga
     2581 acacaggatg agccggtcga cgccggcacg gtcggcggcc tcgagaagcc gctcgggatc
     2641 gccgccgccg ggcgtccgga gatgggcatg acaatcgatc accagggaca ctgggatatt
     2701 ctccttcgtt tgcagtgcgg ctctgccgcg ccgtcgccat tttagaaggc tgcacccgca
     2761 cgttcaacgg aaaagacctg cgaggcgggc cgcggggctg tctgcctcgg cgatcctacg
     2821 attgaatgag ggagagcgct tcatttcacg cccgggtcca agtggcgccg caggcgcccg
     2881 gtatactaaa gtcttcttgc caatgataga atggacgaag gaggcgccca tcatgtcgcg
     2941 cagtttgctg ctggacctca agacgctcgc caagaacctc ttcaaacacc ttcgctatgt
     3001 gctcaatcgc cgcgaatccg ttcgctgcgc tctgtcgggg atggtgtcgg atgtgaagaa
     3061 gctgcgggtg cacgtgggcg cccagcacgc cacagagcgc cgcaaagtcg ccttgaagta
     3121 cgttaaacag ggccgaaccg cctataacgc caaaaacgat gcgctcgccg aaaaactgtt
     3181 tcgtgaggcc gtggctgcgg atgaaacctg tgctctggcc tacgcctacc tcggaaatgc
     3241 gctctaccgg cagggccaga ccgacgaagc cgaagtgagc tggcggcgcg cgaccatgat
     3301 cgatccttct tccgaggggg ccgagaaagc acttcacagt ctccagcatc tcgcccgaaa
     3361 aaagaaagcc tatatggacc atctggatga caaactgcga catggacgtt cttcctgatt
     3421 tttcctattt catgccaacg gagatccggt ttaaagcagg gtgtcttaac gagcttgcgg
     3481 tctgcagccg ggccctggga caaaaaccat ttctgctgac agggcggcat tccgcgcggg
     3541 ccaccggagc cctggaccgg gccctcgccc aactgcccaa tgcggtggtc tacgataagg
     3601 tagaggagaa tccgacgacc ggccaatgcg atgcggcggc acaggtttgc cgggaagccg
     3661 gatgcgatgt ggtcattgcc atcggcggag gcagcccgat ggatgtcgcg aaggccgtgg
     3721 cgggcctggc gctcaatgaa ggcccctgcg cggcatttat cggctccggc ctgtttgcgg
     3781 caggtgcgct gccgatcatc gcggtaccca cgacggcggg cacgggcagc gaggtgacgc
     3841 catacgccgt gctgatcgac gccacggaac atcagaagcg caccatcaag ggccgggccc
     3901 tgtttccccg ggtcgcgctg ctggatccgg agctgacggc gccgcttccg cctgaggtga
     3961 cggtgtatac ggggctcgac gcgctcagcc agggcatgga agggctcgtc tcccggcagc
     4021 ataccgcggt gggggacatc ctggcgcttg aggtctgccg cctggttgcg cggtggctgc
     4081 cgcgcgccgt ccactatccc catgacctcg aagcccgcgg cggcatgctg ctggctgcga
     4141 tgttgtcggg atgcgtcatc gcccagagcg gcacgacgct cgtgcacggc atgggctatt
     4201 actacaccct gcattgcggc ctgcagcacg gccaggccaa cggactgctg cttgcgccgg
     4261 tatttcgaca caatgcggcc cacctgccgg aggccgtcgc gtcgattgcc gcggcactgg
     4321 gctgcgccgg tccaccggag gaggcgggca gcaatatcac gcgcgcgata catgcgctct
     4381 tcgatgcgtg cggggtttca ccggccgcgc gcgacgccgg cgtggatgag agcctgctgg
     4441 cgggttttgc gaaagattgt catgcggatc cctatcgctt caaaaaccag gtgggcgaac
     4501 tgacggagca cgacgtgctg cgcttttacc tcgaatcctg gcggggcgcc tgatgcgcgg
     4561 gcgcaatgcc ttcccttcgg aatgattgcc ggaattcccg gctcgcggac gcggggcggt
     4621 aggcagcacg gatcgggcgt attctccatc ttgttccacc caatcccgcc tgtcaagcca
     4681 tttgctaatc ttaccggggc gtatatcgtc gcgccatttg gt
//
LOCUS       DAOPBQ010000271         4713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15133_length_4713_cov_3.893087, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000271 DAOPBQ010000000
VERSION     DAOPBQ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15133_length_4713_cov_3.893087"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1848)
                     /locus_tag="PKW60_09375"
     CDS             complement(<1..1848)
                     /locus_tag="PKW60_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99682.1"
                     /translation="MITLICAALLAGALTEPASPPFGNVFFPGDAMHINVPADAANLA
                     QTWRALDDTLTMVASGKTSRAGVVGAGALPPGWYRIEFLDGQERCVGFTTAAVLPNRP
                     PSPSQTTPVAVDVALSWLAREESSAWPGLAGLAALAGAGWIRDRIHWREMQLETGAFL
                     EQTKYDRTATVQSSAGLEVLQVFHTMPPWARDAAEGNQRPRGNLRHLYQFCEEMARRF
                     GGRVDAWEPWNEGNADNFGGLTMDELCSLQKAAYLGFKHGNPALTVCWAPLAGANTPA
                     QAEAVLRNETWPYYDVYSIHSYDWPHAYPRLWEPARRAACGRPIWVTECDRGMEVNPA
                     SAVGDFTHDFSRRKAEFMAQSYACSLFSGASRHFHFILGPYTEKQGTIQFGLLRHDYT
                     PRISYVALTAIGRFLADAKCLGRWDATDDPDLHIYAFRSRPNQLPRDILVAWIEKELE
                     WPDRGQQSAPWPLPAELRIEAVFDYLGRPTGHIPPAEIRSAPLFAVLPEGEAATLPLK
                     TLPVSEFRQGKPSPVVLQFDTPGIAPVIRNRAWTPEPERVFEPEMPVSCELVVYNFAN
                     TGQQGTISATRIPDGITIAPDSWDVDLPAGERAVFSSQIIVHASYTKHGQ"
     gene            2003..3142
                     /locus_tag="PKW60_09380"
     CDS             2003..3142
                     /locus_tag="PKW60_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99683.1"
                     /translation="MRTLGLLLALAVALGGTAAGQMPCAGVVTVAARDSSEAMRAAAQ
                     FVCDGEGDQEEINAAVAALPEAGGTVFLAAGTYDIRRVEGELGGILIRRSDVTLQGEG
                     AATKLIQADGQETNVIRIIGSGVGYVTIRDLYVDANRDGNPKSEGDPNISHDRFEFCG
                     IKAFRARPGDPNPTEDTHHITVENCVVFNAARLGIMLEGHGMKVINNRLGNASSDVVE
                     ILTGPGEIRGNYFEITGRTHVAVGSDRGNNILMSDNVVHVRSNGDLDIAFRTWADSHR
                     HVIAGNVIQVDEGGRCTHAMDLRGFDTAVTGNCVYTANQDAPLLLKITAGNVAVTGNV
                     LQNVVIEVDDETPDGKPVLIRDNVLENSSVQVKNGRVLGENDAGE"
     gene            3232..4710
                     /locus_tag="PKW60_09385"
     CDS             3232..4710
                     /locus_tag="PKW60_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HPJ99684.1"
                     /translation="MVIPMRARQCNRRQFLQLAAAGYALSGMPWTGTADAAAQRAPNI
                     VFIMADDLGWNELGCYGQEKIKTPNIDRMAAEGLRFTQHYAGSAVCAPSRCVLMTGKH
                     GGHAYVRDNHQMGKAEDFAGQLPIPADELTCAELLKSRGYVTGCFGKWGLGAPGSSGD
                     PLAQGFDRFFGYNCQSHAHNLYPRFLVNQGEREPLEGNTRGVTGEQYAPQRIADAMLQ
                     FVRDNKEKPFFLYYPTVIPHLALQVPEEELAQYQGLWEETPYTGKNYQPHPTPRACYA
                     AMISFLDKQVGRLFALLKELGLDDNTIVFFTSDNGTTHVIEQVDYEFFDSVGPLNGLK
                     GSLYEGGIRVPMIAWWPGRIAPGTVTDHLAAHYDVLATLADLTGAEAPADTDGISFLP
                     TLLGEGHAQRQHEYLFWDFKGYGGQLAVRMGKWKGIKRDLVKNPDAPLELYNLEDDLS
                     ESTNVAGEHPDVAARLERIMVEARTQPEFEASRFGKYIDAGG"
BASE COUNT          969 a         1336 c         1517 g          891 t
ORIGIN      
        1 ttgtccatgc ttcgtatacg aagcatggac aatgatctgg gagctgaata cagcgcgctc
       61 cccggccggg aggtctacat cccatgagtc gggcgcgatc gttatgccgt ccggaatcct
      121 ggtcgctgaa atcgtcccct gctgccctgt attcgcgaaa ttgtacacaa cgagttcgca
      181 cgagaccggc atttccggtt cgaaaacacg ttcgggctcc ggggtccagg cgcggtttcg
      241 tataacgggc gcgatgcccg gcgtgtcgaa ctgcaagacc acgggtgacg gtttcccttg
      301 acggaattcg gagactggaa gggtcttcag cggcaaggtg gcggcttccc cctccggcag
      361 cacggcaaag agcggcgctg acctgatttc ggccggcggt atatgcccgg ttggccgtcc
      421 gaggtagtcg aaaacggcct cgatgcgcaa ctccgcaggc aacggccagg gggctgattg
      481 ctgcccgcga tcgggccatt ccagttcctt ctcaatccat gcgaccagga tgtcgcgcgg
      541 gagctgattg ggccgggagc ggaatgcgta aatatgcagg tcagggtcat ccgttgcgtc
      601 ccaccgtccc aggcatttcg catccgcgag aaaacggcca atggcagtaa gggcgacata
      661 gcttatgcgg ggcgtgtaat cgtgtcggag cagcccaaac tggatcgttc cctgcttttc
      721 cgtataaggg cccaggatga agtggaaatg gcgcgatgcc cccgaaaaca ggctgcatgc
      781 ataggattgc gccatgaatt cggctttccg acgggagaaa tcgtgcgtga aatcccccac
      841 ggcagacgcc gggttcactt ccatgccgcg atcgcattct gtaacccaga tgggcctgcc
      901 gcacgcggcc cggcgcgccg gttcccagag tcgggggtag gcatgtggcc agtcgtacga
      961 gtggatgctg taaacatcgt aatacggcca ggtttcgttc cggagcacgg cctccgcctg
     1021 tgccggggtg tttgcgccgg cgaggggagc ccagcagacc gtgagggcgg gattgccgtg
     1081 tttgaaaccc agataggcgg ccttctgcag ggagcacagc tcgtccatgg tcaagccgcc
     1141 gaaattgtct gcgtttcctt cattccaggg ttcccaggca tcgacgcggc cgccgaaacg
     1201 ccgggccatt tcttcgcaga actgatagag gtggcgaagg ttgcctctcg gccgctgatt
     1261 gccctccgcc gcgtctcgtg cccagggcgg catggtgtga aaaacctgca ggacctcgag
     1321 tccggcgctg gactgcacgg tggcggttct gtcgtacttg gtctgttcga gaaatgcgcc
     1381 cgtctcgagc tgcatttcgc gccagtgaat gcggtcgcga atccagcccg cccccgcgag
     1441 tgcggccagc cccgccaatc ccggccatgc ggatgactcc tcacgcgcaa gccacgagag
     1501 ggcgacgtcg acggccacgg gggtagtctg ggatggcgat ggcggccgat tggggaggac
     1561 agccgccgtc gtgaatccca cgcaacgctc ctgtccgtca agaaactcga tacggtacca
     1621 tcccggcggg agtgcacccg cgccgactac gcccgcacga gaggtttttc ccgatgcgac
     1681 catcgtgagg gtatcatcga gtgcacgcca cgtctgcgcg agattcgcgg catcggcggg
     1741 cacgttgatg tgcatggcgt caccggggaa aaacacgttg ccgaacggcg gggaggctgg
     1801 ttcggtcagc gctccagcca gcaaagccgc gcaaatcagc gttatcatca cgggccactc
     1861 ctttgttcgc tccggaagct tgtgcctttc ggttcatgga gaatacggga caaaagcagt
     1921 gttggcaaaa cgatttgaac gacacggctc cgcctactag agtgattgcg ggtgcacgac
     1981 atttccagaa ggagctgatg cgatgaggac acttggactt ctgttggcgc ttgccgtggc
     2041 tttgggtgga acggcagcag ggcaaatgcc ctgtgcaggc gtggtgacgg tagccgcgcg
     2101 ggattcatct gaagcgatgc gtgcggccgc gcaattcgtc tgtgacggcg agggagacca
     2161 ggaagaaatc aacgcggcgg tcgcggcgtt acccgaggcc ggggggaccg tttttctggc
     2221 ggcggggacg tatgatatcc ggcgcgtcga aggagaactt ggaggcatcc tcatccgccg
     2281 gagcgatgta acgcttcaag gcgaaggcgc tgcgacgaag ctgatccagg cggatgggca
     2341 ggagacgaac gtcatccgca tcatcggctc gggagtgggc tacgtcacga tccgggacct
     2401 gtacgtcgac gcaaatcgtg acggcaaccc gaaaagcgaa ggggatccca atatttccca
     2461 cgaccgtttc gaattctgcg ggatcaaggc cttccgtgcc cgccccggcg atccgaatcc
     2521 gacggaggac acgcaccaca tcacggtaga gaattgcgtc gtcttcaacg cggcgcgcct
     2581 ggggatcatg ctggagggtc acggcatgaa ggtcatcaac aaccgcctcg gtaacgcctc
     2641 gtcagatgta gtcgagatcc ttacgggccc gggagaaatc cgcgggaatt acttcgagat
     2701 cacgggccgc acgcacgtcg cagttggttc ggatcgggga aacaatatcc tcatgtcgga
     2761 taatgtggtg catgtccgtt cgaatggaga ccttgatatt gcgttccgta cctgggccga
     2821 ttcgcaccgg cacgtgatcg cgggcaacgt catccaggtc gatgaaggag gccgctgcac
     2881 gcacgccatg gatctgcgcg gatttgacac ggcggtcacc ggcaattgcg tctatacagc
     2941 caatcaagac gcgccgctgc ttctcaaaat caccgcaggc aacgtagcgg tcacgggcaa
     3001 cgtcctgcag aacgtagtca ttgaagtgga tgatgaaacg ccggacggga aacccgtgct
     3061 cattcgcgac aatgtgcttg agaattcgtc ggtgcaggtg aaaaatggac gagttctggg
     3121 cgagaatgat gcaggggaat gaggcgcagc ctttgagttg gatttgggga aaaggcgctt
     3181 gaaccggcgg atctgaattt gaggcggtat acagtcccgt gaggaggcaa tgtggtgata
     3241 cctatgcgcg caagacagtg taaccggcgg caattccttc agctggctgc ggcgggatac
     3301 gcgctttcgg gcatgccgtg gacgggcacg gccgatgctg cggcgcagcg ggctcccaac
     3361 atcgtgttca tcatggccga cgacctggga tggaacgagc ttggctgcta cggccaggag
     3421 aagatcaaga cgccaaacat tgaccgtatg gcagcggaag gcctgcggtt cacgcagcat
     3481 tacgccggtt ccgccgtctg cgcgccctcg cgttgcgttc tcatgacggg caaacacggc
     3541 ggccacgcct acgtgcgcga taaccatcag atgggcaaag ccgaggactt tgccggacag
     3601 cttcccattc ccgcggacga gctgacgtgc gcggaattgc tgaaaagccg aggctacgtc
     3661 acaggatgct tcggaaaatg gggtctgggg gcgcccggct cgagcgggga cccgctggcg
     3721 cagggttttg accgcttttt cggctacaat tgccagagtc acgcgcataa tctctatccg
     3781 cgtttcctcg tgaaccaggg cgagcgggaa ccgcttgaag gcaatacccg cggcgtgacg
     3841 ggcgagcagt atgcgccgca gcggatcgcg gacgcgatgt tgcagttcgt gcgcgataac
     3901 aaagaaaaac cgttcttcct ctactatccc acggtgattc cgcacttggc gttgcaggtt
     3961 cccgaagaag agcttgcgca ataccagggc ttgtgggagg aaacgcccta caccggcaag
     4021 aactaccagc cgcatcccac gcccagagcc tgctacgcgg cgatgatctc atttctggac
     4081 aaacaggtgg gccgcttgtt cgcgctgctg aaagagctcg gattggatga caacaccatc
     4141 gtgttcttca cttccgacaa cgggaccacg cacgtgattg aacaagttga ctatgaattt
     4201 ttcgacagcg tggggccgtt gaacgggctg aaaggctcgc tgtatgaagg cggcatccgg
     4261 gtgcccatga tcgcctggtg gccgggaaga atcgcccccg gaaccgtgac ggaccatctg
     4321 gccgcgcatt acgatgtcct tgcgacgctg gctgacctta cgggagccga ggcgcccgcg
     4381 gatacggacg ggatcagttt ccttccgacg cttctgggcg agggccacgc gcaacgccag
     4441 cacgaatacc tcttctggga ttttaaaggc tatggcgggc agcttgccgt gcggatgggc
     4501 aaatggaaag gcatcaaaag ggatttggta aagaatccgg acgcgccact ggaactgtat
     4561 aatctggaag atgacctttc ggagtctacc aacgtggctg gagagcaccc ggacgtcgct
     4621 gcccggctcg aacgcattat ggtggaagcc cggacccagc ctgaattcga agcctcccgt
     4681 ttcggcaaat acatagatgc tggaggataa atc
//
LOCUS       DAOPBQ010000272         4709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15148_length_4709_cov_3.896863, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000272 DAOPBQ010000000
VERSION     DAOPBQ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15148_length_4709_cov_3.896863"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3933)
                     /locus_tag="PKW60_09390"
     CDS             complement(<1..3933)
                     /locus_tag="PKW60_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPJ99685.1"
                     /translation="MVQRLGLTAFCVGALIIGMNPLYAAAAADEENLLRNPSFEEGLS
                     DAGVPLGWRLYGRQDELRTLSLMELPDEEGRALLIADRHPDEEIGIVQRVPATPGTAY
                     VATASSMQMEDASPAGAYLQLRFLPSDEYFQRPLIPGPPGSFNRVEVGLIAPPGTREA
                     EVYLYTHRSPIPQVLVGDVTLTAVAELPPGLGLHNAPKVPEIEALKPLYLETAVAAGG
                     RAAAVIVAPPAYAEQAAVVKAALQACTGAELPVLSDEAVGLPFAQNIITLGNRSTNRF
                     IGLLYDRFYTLLDLRYPGPGGYVVRSLHNPMGDGHNVILLGGSDLAGVSKAAAAFAQH
                     VSAMPPGPSLELPWLMEIELGDGIVCPTDLREFETWDASAAYRSVGYFGWNSISKRMA
                     MYYMTGDAFHAHEALRLAFPDAQAKAEIAEIDGERIENKDLPLGGPYHYNAHLMILFW
                     DLIEESPLFTDDVRLRVTRAFAQQLAHPGIASAYVGPYAAIPTQVGSRHGQWAALSLY
                     CLGRYFAKDYDDPVWSVCEQNGLNHFKSLHAHAWVSGENDNLYWYDTAIAPVFTYLLL
                     SGDRLPLENGVARELLRGLEILASGKDGDWALNYASMGFLHQAAYLMQDGRWLTYRKR
                     TGVDTSIFRLGQSFWPEDHLQPVPPDDMAGSWQIHALPKPMWKARNTGFPLEESFQFM
                     SYRNRPDATGDFILLDGFNGASRNPYHTFAILALRQDGATLLEGYLNQLRTRADGLTE
                     NTIAMDAALKHCRVLGGSVVAVAEVPDMPFTSWRRALLHFPGRYAVVFDELIPRIDTE
                     NLEVQLLWETRGGRWRADPEHPNQLRLDRPLDAPTPRIPVIGTATDVDLFCDRHEATM
                     QWFGPARTGEKLRFFSLVTGVPAEEGSDVACLRLAENAAVLLTPEPAVATIGNYEGIE
                     AEFAVLSETHFYGRNVVQLAVPGLNPVFKSAEPMDIDWDLVRGTMTFAPSSAAAEPPE
                     SRPAPAPAPMLPAVRAWLAACLEQAQARAEHTRGGTDVMEVSLSALQSTVVKAGEAPI
                     ASLVTPPGGAPLFVAEGQTIHALSLSGERMASFSTDGPIRMLHWWPEPQLLLAGCADE
                     KVIAFDQDGNRQWEFVSEMDPEVYRAAKDYWFKSAPGHEGIHGLYSGPFIDGKPMAFV
                     GSACTLEILDETGRLVRRMPQFWGKVSVMKLIPGPDGSHTLLAARRYNGTNTLGIINS
                     VTLDPNPRGFYSVPEGHTHVPGWSAMNREHIFFDDFDGDGKPEVMTEINGVWNRVTVW
                     APDGTAKYDASFGPGKSIPSVNMRDIDIADLTGDGVPDIAVATVDRLCV"
BASE COUNT          863 a         1443 c         1549 g          854 t
ORIGIN      
        1 cacacacaac cgatcgacgg tggccacggc gatgtcgggc acgccgtcgc cggtgaggtc
       61 agcaatgtcg atatcgcgca tgttcacgct ggggatggac ttgcccgggc cgaaactcgc
      121 atcgtatttc gccgtaccgt ccggcgccca tacggtgacg cggttccaca cgccgttgat
      181 ctccgtcatg acctcgggtt tgccatcgcc gtcgaaatcg tcgaaaaaga tgtgctcgcg
      241 gttcatggcg gaccacccgg ggacgtgggt atggccttcc gggacggaat agaatccgcg
      301 ggggttggga tccaaagtca cgctgttaat gatgcccagg gtgttggtgc cgttgtagcg
      361 gcgcgcggcc aacagggtgt gggatccgtc ggggccggga atcagcttca tgaccgagac
      421 tttgccccag aattgcggca tgcgccggac aagccggccg gtttcgtcca gaatctcgag
      481 cgtgcaggcc gaacccacaa atgccatggg ttttccgtca ataaagggcc ccgaatacag
      541 gccgtgaatg ccctcgtgcc ccggcgctga tttgaaccag tagtctttgg ccgcgcgata
      601 cacttccggg tccatctcgg acacaaactc ccattggcgg ttaccatcct ggtcgaatgc
      661 aatgactttt tcgtccgcac acccggccag cagcagttgc ggttcgggcc accagtggag
      721 catgcggatg gggccgtccg tcgaaaacga cgccattcgc tcgcccgaaa gcgacagcgc
      781 gtggatcgtc tggccttcag cgacgaagag cggcgcgccg cccggcggcg taacgaggct
      841 cgcgatcggc gcctcccctg ccttcacaac cgtgctctgc agggcggaaa gggagacttc
      901 cattacgtcc gttccaccgc gtgtgtgttc ggcacgggcc tgggcctgct ccagacaggc
      961 cgccaaccag gcgcgcacgg cgggaagcat cggagcaggt gcaggagcgg ggcgcgactc
     1021 gggcggttcc gcggccgcgg acgagggcgc gaaggtcatc gtgccgcgaa ccagatccca
     1081 atcgatgtcc atgggttccg cgcttttgaa cacgggattc aaccccggca cggccagttg
     1141 gacgacgttc ctgccgtaga agtgcgtttc cgacaatacg gcgaattccg cctcaatgcc
     1201 ttcgtagttg ccgatggtgg cgacggcggg ttcaggggtc aacagaacgg ccgcgttctc
     1261 tgcgaggcgc aggcaggcca cgtcggatcc ttcctcggca ggcacgccgg tgaccagcga
     1321 gaaaaagcgc aatttttctc ccgtgcgggc cgggccgaac cactgcatcg tcgcttcatg
     1381 ccggtcacag aacagatcga cgtccgtagc ggtgccgatc acggggatgc gtggcgttgg
     1441 tgcatcaagt ggccgatcga ggcgaagctg gttcggatgc tccggatccg cacgccacct
     1501 gccgccccgg gtctcccaga gcaactggac ttcgaggttt tcggtgtcga tacgcggtat
     1561 cagctcgtca aacaccaccg cgtagcggcc cgggaaatgg agcagcgcac gccgccagga
     1621 ggtgaacggc atatccggca cttccgccac cgcgacgacc gagccgccca gtacccggca
     1681 gtgtttcaat gccgcgtcca tggcgatcgt attttccgtg agcccatcgg cccgtgtgcg
     1741 gagctggttc aggtatcctt ccagtagcgt ggcgccgtcc tgccgcagcg ccaggatcgc
     1801 gaacgtgtgg tacggattgc gggatgcgcc gttgaaaccg tccaacagga tgaaatctcc
     1861 cgtggcgtcc ggccggttgc ggtagctcat aaactggaac gattcttcga gagggaatcc
     1921 ggtattgcgc gctttccaca tgggtttcgg caaggcatgg atttgccagc tgccggccat
     1981 atcgtccggc ggcacgggct gcaggtggtc ttccggccaa aaggactgcc cgagccggaa
     2041 gatcgaggtg tccacgcccg tccgcttgcg gtacgtgagc cagcggccat cctgcatgag
     2101 ataggcggcc tgatgcagga accccattga ggcatagttc agcgcccagt ccccgtcttt
     2161 ccccgaagcg aggatctcca gtccgcgcaa caattccctg gcgacgccgt tctcgagcgg
     2221 caaccggtcg ccgctcagca gcaggtaggt aaacacgggc gcgatcgcgg tgtcgtacca
     2281 gtacaaattg tcgttttcgc cgctgaccca ggcgtgcgcg tgcagcgatt tgaaatggtt
     2341 caagccattt tgctcgcaaa cggaccagac ggggtcatcg tagtctttcg cgaaataccg
     2401 gccgaggcaa tacagcgaga gcgctgccca ttggccatgg cgggagccga cctgtgtcgg
     2461 gatggccgcg taagggccca cgtaggccga agcaatgccg ggatgggcga gctgctgcgc
     2521 aaaggcgcgg gtcacgcgca agcggacatc gtccgtgaac agcgggctct cctcgatgag
     2581 gtcccagaag aggatcatga ggtgggcgtt gtaatgatag ggcccgccga ggggcagatc
     2641 cttgttttcg atgcgttcgc cgtcaatttc agcgatctcc gccttggcct gagcgtcggg
     2701 aaaggccagg cggagcgctt catgcgcgtg aaacgcgtcg ccggtcatat aatacatggc
     2761 catgcgtttg ctgatgctgt tccatccgaa atagccgacc gaccggtagg ccgccgaggc
     2821 atcccaggtc tcgaattcgc gcagatccgt ggggcagacg atcccgtctc cgagttcgat
     2881 ctccatcagc cacggcagtt caagcgacgg cccaggcggc atcgccgaca cgtgctgcgc
     2941 aaacgccgcg gcggctttgc tgacgcccgc caggtcgctc ccgcccagta agatcacatt
     3001 gtgaccgtcg cccatggggt tgtgaagcga gcggaccaca tatcctccgg gaccggggta
     3061 tcgcaagtcg agcagcgtgt agaagcggtc gtagagcagg ccgatgaacc ggttcgtgga
     3121 gcggttgccg agggtgatga tgttctgagc gaagggcagg cccacggctt catcggatag
     3181 gacgggcaat tcggcgccgg tgcaggcctg gagcgccgcc ttcaccactg ccgcctgttc
     3241 cgcataggcg ggcggcgcca cgataaccgc ggccgcgcgt cctccggcag ccacggccgt
     3301 ctcgaggtag agaggtttga gcgcttcgat ttcaggcact ttgggggcgt tgtgcaatcc
     3361 caggccgggc gggagctccg ccacggccgt cagcgtgacg tcgcccacga gcacctgcgg
     3421 gatgggactg cgatgagtat acagatacac ctccgcttcc cgtgtgcccg gcggtgcgat
     3481 caagcccact tcgacgcggt tgaacgagcc cggcggaccg gggatgagcg gccgttgaaa
     3541 atattcgtcc gacggcagga atcgcagctg gagataggcc ccggccggcg aggcgtcttc
     3601 catctgcatg gaagatgccg tcgccacata ggccgtgccg ggcgtggctg gcacccgttg
     3661 cacaatgcca atctcttcat cgggatgcct gtcggcgatc agcagggccc ggccttcttc
     3721 gtccggcagt tccatcaacg acaaggtccg cagctcgtcc tgccggccgt acagtctcca
     3781 gcccaggggc accccggcat ccgacaggcc ctcctcgaac gaggggttgc gaaggaggtt
     3841 ttcttcatcc gctgcggccg cggcgtacag ggggttcatg ccgataatga gcgcgcccac
     3901 gcaaaatgct gtcaagccaa ggcgctggac cataccgtgt tcctccttca gattgagaga
     3961 ggcgccgtac cccgaaaccg cgtcgcgcga ccccggttca atcttattcg aaagcaggca
     4021 tgaagtcatc caggcgccgg attgtgtctg cggcgccggt cagggcgtat gagtgcatgg
     4081 cctctcccgt taccgcagac ttcgcgggag atgcgcgcca cccgcggggg atgggcggag
     4141 atcagcgtga cagaggcctg cccctgcaca cgcgccctgc tggcgggggt ggacgtctgc
     4201 cgggccaaga ccctggcatg cggcacgcgt ggacggagtg ggcatctacg cggcaaaggc
     4261 tcgggcgcgc gaatcgcccg gtttgattcg cgccgcccgt ctggtaaaat tcacttgatc
     4321 agaaggctgg cgcgagacat tcaaggttgt ggggtgcggg attcgcgtca ttctgatctg
     4381 ccgccgtgcg tagaggctat acgagaccct ttcatgcagc agacttcgcg cgcggagcgt
     4441 ccggaagaat cgggaaagca cagcgcggag cgggtgcgca aggccgcttg aatcgactcc
     4501 ggagcggggc agatattcat tcgcaggcgc tggcacagtc tcgtgatctg aacatcgcaa
     4561 ttttattcgt gccgctcaat ccgcgcggag ggaggcgtga tcatgctcaa ggtggccttt
     4621 gtgggctccg ggggaatcca gggtgtgcat tgcgccaatc tcagccgcaa tccggacgtg
     4681 aacattgtgg ggcactgcga catcgagtt
//
LOCUS       DAOPBQ010000273         4708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15155_length_4708_cov_3.692242, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000273 DAOPBQ010000000
VERSION     DAOPBQ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15155_length_4708_cov_3.692242"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="PKW60_09395"
     CDS             complement(<1..149)
                     /locus_tag="PKW60_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002279707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HPJ99686.1"
                     /translation="MKRALQFGAGNIGRGFLGQLYYESGYAATFVDVLDDVVRALNER
                     GEYPLR"
     gene            complement(241..1032)
                     /locus_tag="PKW60_09400"
     CDS             complement(241..1032)
                     /locus_tag="PKW60_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HPJ99687.1"
                     /translation="MTEIELIAQILEVLGPYVRERRADRENLTISSKSHANDLLTEVD
                     IETQRRIVARIGEAFPGDLIVGEEEGYARYPGEPGGRTWIIDPIDGTQNFVRSLLPEF
                     GISIAFARGNELLAGGVAFPGTGDVFLAERGSGAEQNGRPIRVSEVNHLSLARVELDF
                     DGPSKRQATLDRARSILLNAGQVRCHCAAVLGLCSIASGDADAYVHVSLRPWDYAASL
                     LLAEEAGGASSSLSGDPLQLFGENDGILVSNGRIHAELLAHVRRS"
     gene            complement(1052..2044)
                     /locus_tag="PKW60_09405"
     CDS             complement(1052..2044)
                     /locus_tag="PKW60_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPJ99688.1"
                     /translation="MASKMFQTLLVYAVLLALSAVFISPFLWMLSTSLKHESRIFPKA
                     GEPPQWIPTTTLLDASGHPMVVYEGREGVDLGPDEMGRHVLRLGSEEVAVWPEDFEVR
                     QRLGLHWQNYTEAFYKMAFWQNLRNTLFICVTVVLGTLVSCSLVAYSFARIPWPGRDA
                     VFVLVLATMMLPYQVTLIPLFALYRELGWVGTFKPLIVPAFFGTPFYIFLLRQFFLGV
                     PQDLSASARIDGCNEFGIYLFIMLPLSKPALATTALFMFLFQWADFLDPLVYLQDDRQ
                     YTLAIALQQFQSQHESAWGPLMAMSSVITIPIILLFFLTQRTFIQGITLTGLKG"
     gene            complement(2034..2927)
                     /locus_tag="PKW60_09410"
     CDS             complement(2034..2927)
                     /locus_tag="PKW60_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPJ99689.1"
                     /translation="MTRMERRNLRNGMLFALPYLIGGSVFMVYPLAMSVYYSLCHYNI
                     ITPPTFAGLDNYRVLFFHDDVFWKSVYNTLYFTAFSVPLSLFLAIGLAVLLNRAVKGL
                     SVFRTIFFLPTIVPVVASAVLWLWVLNPESGLINGMLREFLNIEGPGWIADVRWSKPS
                     LIVMSLWAVGGPMVIFLAGLADVPAVLYEAAEIDGAGPWQQFRNVTLPMLTPTILFNL
                     VMGLIAAFQYFTQVYVMTAGKGTPADSTMFYALYLYRNSFVYLKMGYASAMALLLFLV
                     ILAATVGVLISSKRWVHYHGE"
     gene            complement(2924..4312)
                     /locus_tag="PKW60_09415"
     CDS             complement(2924..4312)
                     /locus_tag="PKW60_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPJ99690.1"
                     /translation="MKSTYMRWILALAVSLAVVAALWPSEAGSPEEKAALEEGRIVIT
                     YWDRHTGHEFDERKVLIDEFNASQSEVYVRALPIGFTMEKLLTSIAAGTPPDILSTEG
                     TILDALAPQHCVMPLDTFIEKSESIEPDDFFPHLWRAVTFDGHTWGIPVTTDSICLLW
                     NKAAFRRAGLDPERPPKTMEELLEYASKLTVRDGAGLKQIGFLPWLPWDHTHMWGGLF
                     GGQWFDDVSGRFVCGDDPQILAMFRWMQSFSIDPAAPAQLPYALNPEMVAAFQTGFGA
                     YQSANNPFYSGKVAMTQEGEWQTSFIKRYAPELDWGVAPLPQPEGAPPRCYSPTCVLD
                     LIPAGAPHPEAAWKYIEWFNSPRPGGQPSPASDYNHAICNIPCRRADAMHERVLGDPA
                     MSVFVNELLTKEPLVYPVTPVAQFFIDQVERERGLVILRQRTPEQAARAIEDSANAEL
                     DRILTIMRHERP"
     gene            4496..>4708
                     /locus_tag="PKW60_09420"
     CDS             4496..>4708
                     /locus_tag="PKW60_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPJ99691.1"
                     /translation="MAEKRQDIRPVRVLPEDVANKIAAGEVVERPGSVVKELIENAFD
                     AGASRIIVRLVAAGRRTIEVIDNGHGM"
BASE COUNT         1014 a         1443 c         1405 g          846 t
ORIGIN      
        1 cgcagagggt attccccccg ttcgttgagc gcccggacca cgtcgtccag cacgtccacg
       61 aacgttgccg cataccccga ctcgtaatag agttgtccga gaaatccccg gccgatattc
      121 cccgccccga attgcagagc ccgtttcaca ggtgttcctt tccagttcca ggtttgggta
      181 cgaaggggca tcaagtatac cggcgctggc aggagtttgc gatttttgaa gacagcccgt
      241 tcaggaccgg cgcacgtgcg ccagcagttc tgcgtgaatc cggccgttgc tgacaaggat
      301 gccgtcgttc tcgccgaaca gctgcagcgg atcaccgctc aaactcgatg aagcgccgcc
      361 ggcctcttcc gcaagtagaa ggctcgccgc gtaatcccac ggccgcaatg acacgtgcac
      421 gtaggcatcc gcatcgccgg acgcaatcga acagagtccc agcaccgccg cgcaatggca
      481 gcggacctgg cccgcgttca acaggatgct ccgggcgcgg tcgagcgtgg cctggcgttt
      541 ggatggaccg tcaaaatcaa gctcaacgcg ggccagcgac aggtgattaa cttcggaaac
      601 gcggatgggg cgcccgttct gctccgcacc cgagccccgc tccgccagaa atacatctcc
      661 cgtgcccgga aacgctacgc cgcccgccag gagttcattg ccccgggcga acgcgatcga
      721 tatcccgaat tcgggcagca gcgagcggac aaaattctgg gtgccgtcaa tgggatcgat
      781 gatccaggtg cggccgccgg gttccccggg atagcgggcg tagccttcct cttctccgac
      841 gatcaggtcg ccgggaaagg cctcgccgat ccgggccacg atccggcgct gggtctcaat
      901 atccacttcg gtgaggagat cgtttgcgtg gctcttcgat gagatggtga gattctcgcg
      961 atcagcccgc cgttcccgca cataagggcc gagaacttcc agaatttggg caatcaactc
     1021 gatttcggtc atgggaattc tccggttggc atcatccttt gaggccggtc agggtgatgc
     1081 cctggataaa cgtgcgctgg gtcaggaaaa acaggaggat gatgggaatg gtgatcacgc
     1141 tggacatggc catgagtggc ccccacgcgc tttcgtgctg gctttggaac tgctgcaagg
     1201 cgatcgccag cgtgtattgg cgatcatcct ggaggtatac gagtggatcc agaaagtcag
     1261 cccattggaa tagaaacatg aacagtgccg tggtggccag ggcgggcttg gaaagcggca
     1321 gcatgataaa cagatagatg ccgaactcgt tgcagccatc gatgcgggcc gatgcgctca
     1381 ggtcctgagg cactcccaga aagaactggc gcagaaggaa aatataaaac ggcgtgccaa
     1441 agaaggcagg gacaatgagc ggtttgaacg tcccaaccca gcccagctcg cggtaaaggg
     1501 cgaaaagcgg gatcaaggtg acctggtacg gcagcatcat cgtcgccaaa accagcacaa
     1561 acactgcatc gcgcccaggc cacggaatcc tcgcgaaact gtaggccacc aatgaacagg
     1621 aaaccagcgt tcccagcacc accgtcacgc aaatgaacag cgtgttgcgg aggttctgcc
     1681 agaacgccat cttgtagaag gcctccgtat agttttgcca gtggagcccc agccgctggc
     1741 ggacttcgaa gtcttcgggc cagacggcca cttcctccga accgagacgg agcacgtggc
     1801 ggcccatctc atcaggcccc aggtccactc cttcccggcc ttcatagacc accatggggt
     1861 gtcccgaggc gtcgaggagg gtcgtggtgg gtatccattg cgggggctcg cccgctttcg
     1921 ggaagatgcg cgactcgtgt ttcagcgagg tcgacagcat ccagaggaac ggcgagatga
     1981 acaccgccga cagggccagc agtacggcgt agacaaggag cgtctggaac atcttactcg
     2041 ccatggtaat gcacccagcg ttttgacgag atcagcacgc cgaccgtagc cgccagaatc
     2101 acgaggaaaa gcagcagggc catggccgag gcatagccca ttttcaggta cacgaacgag
     2161 tttcgataaa ggtacagcgc gtagaacatg gtgctgtcgg caggcgtgcc tttacccgcg
     2221 gtcatcacgt agacctgggt gaaatactgg aatgccgcga tcagccccat caccagattg
     2281 aacaggatcg tcggcgtcag catgggcagg gtcacattgc ggaactgctg ccaggggccg
     2341 gcgccgtcaa tctccgccgc ctcgtacaac accgccggca cgtctgccag acccgccaaa
     2401 aagatcacca tcgggccgcc cacggcccac aggctcatca cgatcaacga tggcttcgac
     2461 caccgcacgt ccgcaatcca tcccggacct tcgatattca gaaattcacg cagcattccg
     2521 ttgatcagcc cgctctcagg gttcaggacc cacagccaga gcaccgccga cgccaccacc
     2581 ggcacaatgg tcgggagaaa gaagatcgtg cggaagacag acagcccttt caccgcgcgg
     2641 ttgagcagca cggccaaacc gatcgccagg aagaggctca acggcaccga aaacgccgtg
     2701 aaatacagtg tgttgtaaac ggatttccag aacacgtcgt catggaaaaa caacacccgg
     2761 tagttgtcca ggcccgcaaa cgtgggcggc gtgatgatgt tgtaatggca caggctgtag
     2821 tacacgctca tcgccagcgg atagaccatg aacaccgaac cgccaatcag gtagggcagt
     2881 gcgaacagca tcccgttgcg caggtttctc cgttccattc gagtcatggc cgctcgtgcc
     2941 tcataatcgt cagaatccgg tccagttccg cgttggcgct gtcctcaatt gcgcgcgcgg
     3001 cctgctccgg ggtccgttgg cgcaggatca ccagcccgcg ctcccgttcg acctggtcaa
     3061 tgaagaactg agccacgggc gtaacgggat acaccagggg ctccttggtc aaaagttcgt
     3121 tgacaaacac cgacatcgcc gggtcgccca gaacacgttc gtgcatggcg tctgcgcggc
     3181 ggcaggggat gttgcagatc gcgtgattat agtcgctggc gggggagggt tgtcctcccg
     3241 ggcgggggct gttgaaccac tcgatgtact tccaggctgc ctcggggtgc ggcgcgcccg
     3301 ccgggatcag gtccagcacg catgtaggtg aataacagcg cggtggcgcg ccttccggct
     3361 gagggagcgg cgcaacgccc cagtcgagtt ccggtgcata ccgtttgatg aaggaggtct
     3421 gccactcgcc ctcctgcgtc atggcaacct tccccgaata gaacgggttg ttcgcgctct
     3481 ggtaggcgcc gaatccggtc tggaacgccg ccaccatctc aggattcagc gcatagggga
     3541 gctgggccgg cgcggccgga tcgatcgaaa aactctgcat ccagcggaac atggcgagaa
     3601 tctgcggatc atcgccgcag acaaaccgcc ctgagacatc gtcaaaccac tgccctccga
     3661 acaagccgcc ccacatgtgc gtgtggtccc agggcagcca cggcagaaaa ccgatctgtt
     3721 tgagcccggc tccgtcgcgc acggtcagct tgctcgcgta ctcaagcagc tcctccatgg
     3781 ttttcggagg acgctccgga tcgaggcctg ccctgcggaa cgccgccttg ttccatagga
     3841 gacagatgct gtccgtcgtg acgggaatcc cccaggtgtg cccgtcaaac gtgaccgcgc
     3901 gccataaatg cgggaaaaag tcgtccggct caatcgattc ggacttctcg ataaacgtgt
     3961 cgagcggcat gacgcagtgc tgcggcgcca gtgcatccag aatggtccct tcggtgctca
     4021 ggatatccgg aggcgtcccg gctgcgatgg aagtcagcag tttttccatc gtgaacccga
     4081 tggggagcgc gcgcacgtac acttcgctct gcgacgcgtt gaattcgtct atcagcacct
     4141 tgcgctcatc gaattcgtgc ccggtgtggc ggtcccaata ggtaataacg atgcggcctt
     4201 cctcgagcgc ggccttttcc tccggcgacc cggcctccga cggccacaga gcggcaacca
     4261 cggccaggct gaccgccagt gccagaatcc agcgcatgta ggtggatttc acgggtttgt
     4321 gtctcgctca gataccgttt ttatcgaaga tgaaaaccca ttatgcaacc tgtggcaaac
     4381 cggaatccac tgttcaaccc gcaccttccc gaggcaaggt ccgcgcgcgc agcttgcggc
     4441 tgtccccggg cttcaataca atgaaactcc agcgtcaacc ctgggcaaca acactatggc
     4501 tgagaaaaga caggatatcc ggcccgttcg cgtgcttccg gaagatgtgg ccaataaaat
     4561 tgccgccggc gaggtcgtgg aacggcccgg ttcagtcgtt aaagagctca tcgaaaatgc
     4621 cttcgacgcc ggtgccagcc ggatcatcgt ccgcctggtc gcggcgggac gccgcacgat
     4681 cgaggttatc gataacggcc atggcatg
//
LOCUS       DAOPBQ010000274         4708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15156_length_4708_cov_3.602622, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000274 DAOPBQ010000000
VERSION     DAOPBQ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15156_length_4708_cov_3.602622"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..680)
                     /gene="raiA"
                     /locus_tag="PKW60_09425"
     CDS             complement(111..680)
                     /gene="raiA"
                     /locus_tag="PKW60_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPJ99692.1"
                     /translation="MQLDISARHMELTDALRSHVQTRLEKLRGHFDRVIDVNVVLSVE
                     KHRHVAEVTLHANGVRMHGKESSLDMYASVDAVVEKLQKQIRKYKDRVSRHGSRKGMD
                     SLAGMPEELLEGVTEEIQEELDEPVPLPQRQIQREKMPMKPMKIEEASFQLELAEEPF
                     IVFSNADTQQVNVMYARKDGTYGLIEPQF"
     gene            complement(833..4102)
                     /locus_tag="PKW60_09430"
     CDS             complement(833..4102)
                     /locus_tag="PKW60_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPJ99693.1"
                     /translation="MNSIRLGLPLDGEWLLACDPADVGIQEQWFAKPPPADSIRVAVP
                     SVWDLWLPDYDGAGWYYRTFEIADEWLDYYATLEFDAVDYFAQVWLNGTPLGFHEGGY
                     TPFSLPASSALRTGPNQLVVRVIDPHGPGGYGDFHPRQIPCSREEDYFPFAGIWGSVR
                     LLGRNPIHLIDVFLEPDPRRKRVAATVTAAQPGEVRLTIAGTGLPVTGAPGRLTVEFP
                     DFEYWCPENPKLYVMHCELLREGRVMDAMAVRFGMREFTVKEGRFFLNSQPLFLKGVL
                     QQPDYPRTLAAPATEELARRELMLARDAGFNLVRMHLKPPPPLTLDLADELGLLVLEE
                     PPIGRLDDSRWMRERCEREVREMILRDRNHPSVVMWGILNESGNADLRANRGLQSIKS
                     DLARLARSLDPSRVILDDSGGASITREPSRLMRPYREDFESYDDLHTCQRAPVDREIE
                     QYYEHIGTPNALCFLSEFGFGGMEDLPDVLEQYGPEREHLKDARFVRKVCDTVMDGFA
                     QRELERVFGSFSGFARAAQELQCDAIQSQLDALLANSKLAGYCYTQLCDAGRDFCAGM
                     LDRWRRPKPALEALRSAQQPLRPLIRVERTNLSPRQEVQVRVLIVNHPRTEGRADLSL
                     QVVGPTNQVLWKKKRNVKLPRTGKELWNGTISASGSPGTHKFVVRLMQGMKVLGETTL
                     DFDVLLPAEPCTEEVHLLDPAREWDEVVATLAKKGGPRAPVHIVPPLANSIRAYPADE
                     LCSVLGEVREGAVALFFGPPDDWNDFAAYLDGAPAATGKDSIGSSLGVYHYVKLHPVF
                     EKLPARSLMRQVYRNIVPARTFIEPSDEDICGCLDTRALAAVNATPEETAPWGDNILV
                     RRFGAGRLIFTHLRVFENLGRDPVADRLLVNFVRHFARRSVPSPEPAAIPRPARDWLV
                     RERSTNLRRWKVLGTFPNWEGAGHDQAYPPEHGVDFTAEYQGWYKPIAWRDWYTLARD
                     RHVLDLQAALSPLLESHSRVECGTAYAYTEVYSDVRQQAVLRFHVQNATRAWLNGNPV
                     CDKNIHLPSGEFETCETEGLLRQGRNVILVKVSTVPGEFRFALDIESSEGKPLEVKWW
                     R"
     gene            complement(4201..>4708)
                     /locus_tag="PKW60_09435"
     CDS             complement(4201..>4708)
                     /locus_tag="PKW60_09435"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HPJ99694.1"
                     /translation="LFLAIVGWALFLIAVLTGIVLDLMGLFGNWVILGAAAIAWIVTG
                     FEHFGFLALGVMSALALLGELIESGAAGLGASQCGGSRGSIIAALIGCVAGAAVGTPL
                     FPVVGTVAGACLGAFIGAALHEYLNMERTAGQSMRVGAGAAMGRLFGLLAKTLVGFIM
                     LLVAALSY"
BASE COUNT          956 a         1483 c         1350 g          919 t
ORIGIN      
        1 attccgtgag ctgatcgagc acgcgggcaa cggcaatact ctctttacga ttgaccggga
       61 ggctcttggt gtccattctc actccgcgcg catgccctgg ctgcccagct ctagaactgc
      121 ggttcgatca ggccataggt accatctttg cgggcataca tcacattgac ttgctgggta
      181 tccgcgttgg aaaagacgat gaatggttcc tctgccagtt cgagctggaa cgaagcctct
      241 tcgatcttca tcggcttcat gggcatcttt tcgcgctgga tctgcctttg cggcaggggc
      301 acgggttcat ccagttcttc ctggatctct tccgtcacgc cttcaagcaa ttcttccggc
      361 atcccagcca ggctatccat gcccttccgc gaaccgtgac ggcttacccg atccttgtat
      421 ttccggatct gtttctggag tttctcgacc accgcgtcga ccgaggcata catgtcgagc
      481 gatgactcct tgccgtgcat gcgcacgccg ttcgcatgga gcgtcacctc agccacatgg
      541 cgatgcttct ccacgctcag gacgacattg acatcaatca ctctgtcaaa gtgtccccga
      601 agcttttcga ggcgagtctg cacgtgcgat ctgagcgcat ccgtcagttc catatggcgc
      661 gccgaaatat ccagttgcat aattccttta tcctccacta cttagagcga tttgagccgt
      721 gcagagatcg tagcaccaat tacagggagc tgtcaatccg ggcagcggtt cacctttcat
      781 ctgctcatac atcccgcggt cgatgcacat tcccggcgac cggagagggg gtctatcgcc
      841 accatttcac ctccaggggc ttgccctctg aggattcgat gtccagggcg aaccggaatt
      901 cgccggggac tgtcgacact ttcaccagta tgacattgcg gccctgccgc aacaaccctt
      961 ccgtttcgca ggtttcgaat tcgccgctcg gcaggtggat gttcttatca cataccggat
     1021 ttccattgag ccaggcccgg gttgcatttt gcacgtgaaa gcgcaagacg gcttgctgcc
     1081 gcacgtcgct gtacacttca gtataggcat aagccgtacc gcattcgacg cgtgaatgcg
     1141 actcgaggag aggggagagt gcagcctgca agtccagcac atgcctgtcc cgcgcgaggg
     1201 tataccagtc acgccaggcg atcggcttgt accatccctg gtactccgcc gtgaaatcga
     1261 cgccgtgttc aggcggatag gcctggtcat gcccggctcc ctcccaattg gggaacgtgc
     1321 ccagcacttt ccagcggcgc aaatttgtgg aacgctcgcg cacaagccaa tcgcgtgccg
     1381 gacgcggtat ggccgcaggt tcaggcgacg gcacgctgcg ccgggcaaag tgccgcacaa
     1441 aattcaccag aaggcggtca gcgacgggat cgcggcccag attttcaaaa acccgcaagt
     1501 gggtgaatat gagccggcct gccccaaagc gccgtaccag aatattgtct ccccaggggg
     1561 cggtctcttc aggcgtggcg ttcacggccg caagagcccg tgtatcgaga caaccgcaga
     1621 tgtcttcgtc tgaaggctca atgaaggttc gagcagggac gatattgcgg tagacctggc
     1681 gcatgaggga tctcgccgga agcttttcga atacgggatg caatttgacg tagtggtaca
     1741 cgccgagaga gctcccaatg gaatctttgc cggtcgcggc gggcgcaccg tccaggtatg
     1801 cggcaaaatc gttccagtcg tccggcgggc cgaagaagag ggcaactgcg ccttcacgca
     1861 cttcgcccag cacggagcac agttcatcgg cggggtaggc ccgaatactg ttggccagcg
     1921 gcggcacaat atgcacgggc gcccgcggcc ctcctttttt tgccagggtt gctacaacct
     1981 catcccattc tctggcagga tccagcagat gcacttcctc cgtgcatggt tccgctggga
     2041 gaagcacatc aaaatccagg gtcgtctcgc ccagcacctt catgccctgc atcagacgca
     2101 ctacgaattt atgcgtcccg ggggagcccg acgcggagat ggtcccgttc cacagttctt
     2161 tgccggtacg cggcagtttt acattgcgtt tctttttcca caagacctgg ttagtcgggc
     2221 ccacaacctg gagggacagg tccgcgcgtc cttcggtgcg cggatggttt acgatcaaca
     2281 cccgcacctg gacttcctgg cggggcgata aatttgtgcg ttctacgcgg atgagaggcc
     2341 ggagcggctg ctgcgccgat cgaagagcct ccagtgcggg tttgggccgc cgccagcgat
     2401 ccagcatgcc ggcgcaaaaa tcacggcccg catcgcagag ctgggtatag caataaccgg
     2461 ccagcttcga attcgccagc agggcgtcga gctgagattg gattgcatcg cactgcagct
     2521 cctgcgctgc acgggcaaaa cccgaaaatg agccgaatac gcgctcaagc tcacgctgtg
     2581 caaagccgtc catcaccgta tcacatactt tccgcacgaa gcgggcgtct ttcaaatgct
     2641 cgcgttccgg gccgtactgt tcgagaacgt ccgggaggtc ttccatgccg ccgaagccga
     2701 attccgacag gaaacacagg gcgtttgggg tgccgatgtg ttcataatac tgctcgattt
     2761 cgcggtcaac cggcgcgcgc tggcaggtgt ggaggtcatc atatgactca aagtcctcgc
     2821 ggtacggccg catcaggcgc gacggctcgc gtgtaatgct ggcgccgccc gagtcgtcga
     2881 ggatcacgcg gctggggtcg aggctccggg cgaggcgggc gagatcgctc ttgatgctct
     2941 ggagcccacg gttagcccgt agatctgcat tgcctgattc gttcaggatc ccccacatca
     3001 ccacgctggg gtggttgcga tcgcggagga tcatctcacg aacctcgcgt tcgcatcgct
     3061 cgcgcatcca tcgtgaatcg tcgagccggc caatcggcgg ctcttcaagc accagcagcc
     3121 ccagttcatc cgcgaggtca agcgtaagcg ggggaggggg tttgaggtgc atgcgcacga
     3181 ggttgaatcc cgcatcccgg gccagcatca attcgcggcg cgcgagctct tcggtggcgg
     3241 gcgccgccag ggtgcgcggg taatcgggct gctgcaacac gcctttcaag aagagcggtt
     3301 ggctgttcag gaagaatcgc ccctctttga cggtgaattc gcgcatgcca aaacgcaccg
     3361 ccatggcatc cataacccgc ccttcgcgca gcagctcgca gtgcatgacg tagagtttcg
     3421 ggttttccgg acaccagtat tcaaaatcgg gaaactcgac ggtcaggcgg cctggcgccc
     3481 ccgtcaccgg caggccggtt cctgcaatgg tcaggcggac ctctccaggc tgtgccgcgg
     3541 ttacggtagc cgcgacccgt tttcgccgcg gatccggttc cagaaagacg tcaatcaggt
     3601 gaatgggatt acgccccagt aaacgcacag agccccatat ccccgcgaat gggaagtagt
     3661 cttcttccct ggagcacgga atctggcggg gatggaaatc accgtagccg ccggggccat
     3721 ggggatcaat gacacgcact accagttggt tggggccagt tcgcaacgcg ctgctggcgg
     3781 gcagggagaa aggcgtgtag ccgccttcat gaaatcccag aggagtaccg ttcagccata
     3841 cctgagcgaa atagtctacc gcatcaaatt ccagcgtcgc gtaataatcc agccattcgt
     3901 cggctatctc aaaagtccgg taataccagc cggccccgtc gtagtcaggc agccagagat
     3961 cccagacgct ggggaccgcc acgcggatcg aatccgcggg aggcggtttt gcgaaccact
     4021 gctcttgaat ccccacgtca gccggatcgc acgccagcaa ccactcccca tcgagaggca
     4081 accccaaccg tattgaattc atacttccat ccagcgtaca cgcgcgtacg tcacgaagga
     4141 atcaccggcc aaacccaacc gtctcctggc ctcacggcag ccgtgttcct gccgcactgc
     4201 ctagtaactc aaggctgcca caagcagcat aataaagcct accagcgtct tggccaagag
     4261 gccaaacagc ctgcccatag cggcaccggc gccgaccctc atcgactggc ctgccgtgcg
     4321 ttccatgttc aggtactcgt ggagcgccgc tccgatgaag gcccccaggc aggcccctgc
     4381 caccgtgccg accacgggga acaggggagt ccccacggcg gcccccgcca cgcatccgat
     4441 gagcgcggcg atgatcgagc ctctgctccc gccacattgt gacgcgccga gcccggccgc
     4501 cccgctttcg attagttcac ccagaagcgc cagggctgac atcacgccca gcgccaggaa
     4561 accgaaatgc tcgaatcccg tcactatcca ggctatcgcc gcggccccga gaatcaccca
     4621 gttgccgaac aggcccatga gatccagcac aatgccggta agaactgcga tcaaaaacag
     4681 cgcccagccg acgatggcca aaaacagc
//
LOCUS       DAOPBQ010000275         4706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15160_length_4706_cov_2.923887, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000275 DAOPBQ010000000
VERSION     DAOPBQ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15160_length_4706_cov_2.923887"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PKW60_09440"
     CDS             <1..1033
                     /locus_tag="PKW60_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99695.1"
                     /translation="PADPAAAVERVLESDPSLLAQHRGYQAWIGRYPAAASAEEAFTT
                     LLLTTGFSLPVDRFDEALVRFPESAAAMDAYYAALASNGEIRAAVDDLHRFELAQRAG
                     REAWDGILAYLRAHPEEALTFLKNPQWAKPTPDALRPLADAARKKDPSLDRLRAAFQA
                     LHDQPEAHLHVFPWWKAYCGEESLIREPFDALMAHFRQYPHHFWVWHRREIVLAETPG
                     VRAWLRHWHRTVRRSEGLGRAYWLYLDGLRTQPEHAEAAEARWRSEFGTVSPWPPDNE
                     PPRLTPLRSPSRAAPRVERPPRVDPPVPRRPHIPTSEVVKRPARPIPPTRPVKDTEKT
                     GQDAEPGKP"
     gene            complement(1040..1243)
                     /locus_tag="PKW60_09445"
     CDS             complement(1040..1243)
                     /locus_tag="PKW60_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HPJ99696.1"
                     /translation="MAKSCPNHEKNMAFCTCSYPGCSNHALCCECIQSHLRSRELPGC
                     CFPPEAEKTYDRSFEAFARAWNL"
     gene            1524..2108
                     /locus_tag="PKW60_09450"
     CDS             1524..2108
                     /locus_tag="PKW60_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99697.1"
                     /translation="MKRTALVAACLTMALIVGCGGKSAEKASEALAERMIERAARESG
                     GNIDVDISSGKIAVKGVDEHGEAVNFTVDTDTGTMTSDNGAVSFATGEAAKIPDDFPK
                     DVPLYKGAQLVSVHRDANAGMTSLAATSGDPASTVAEFYKKQAEANGWTQETVFSQSD
                     SMHMMSYEKDSRELSVVIAGEGGATQIQLTLAQQ"
     gene            2263..2769
                     /locus_tag="PKW60_09455"
                     /pseudo
     CDS             2263..2769
                     /locus_tag="PKW60_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
     gene            3350..3859
                     /locus_tag="PKW60_09460"
     CDS             3350..3859
                     /locus_tag="PKW60_09460"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPJ99698.1"
                     /translation="MKCPFCAENISDDAIKCRFCGEFLQGRPAALSEQLKHPAPEELI
                     WSGRSSKWAYLIPYLCLGVVLLPVFGVGLVFFPIAFHKHFRKRYEVTNRRIRARVGLL
                     TTSVHEVAIRDIRYVHIHRSFKDKCIGTASLQFASAGHAGIEVSFISIEKWRTIADYI
                     EDCRAIASH"
     gene            4231..>4706
                     /locus_tag="PKW60_09465"
     CDS             4231..>4706
                     /locus_tag="PKW60_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99699.1"
                     /translation="MRQTPLLDEHKALGGKVIDFSGWALPVQFAGILQEHTHTRSSAS
                     IFDCSHMGEFRIKGREAIEAYDALITTDTGRIRPGRARYGALLTGDGGYIDDLITMRL
                     GGDELFVVTNAGSLDRVAAVFASQVPGLVDESAETAKIDIQGPLSREILVSIGLTAA"
BASE COUNT          965 a         1435 c         1384 g          922 t
ORIGIN      
        1 gcccgcagat ccggcggcag cggtcgagcg cgtcctcgag agtgacccca gcctgcttgc
       61 gcagcaccgg ggctatcagg catggatcgg acggtatccc gccgccgcct cagcggagga
      121 ggcgtttaca acgctgctgc tcacgacagg gttttctctg cccgtcgacc gattcgacga
      181 agccctcgtg cggttcccgg aaagtgcagc agccatggat gcgtattatg cggctcttgc
      241 atcgaatggg gagatccggg ctgccgtgga cgatctccac cgctttgaac tggcccagcg
      301 ggccgggcgc gaagcctggg acggaattct ggcctatctg cgcgcccacc ccgaggaggc
      361 gctgacgttt ctcaaaaacc cgcaatgggc caaacccacc ccggacgcgc tccgtcctct
      421 ggcggatgct gcacgaaaga aagacccgtc gcttgatcgc ctgcgggcgg cgtttcaggc
      481 cctgcacgac cagccggaag cccacctgca cgtgttcccg tggtggaagg cttactgcgg
      541 cgaggagagc ctcatccgcg agccgttcga cgccctgatg gcgcatttcc ggcagtatcc
      601 tcatcatttc tgggtctggc accgccgtga gatcgttttg gcggagactc ccggcgtgcg
      661 tgcctggctc cggcattggc accgcacggt gcggcgctca gaaggtctgg gccgcgccta
      721 ctggctctat ctggatggcc tgcgtacgca gccggagcac gccgaggccg ccgaggcgcg
      781 ctggcgcagc gagttcggca ctgtttcccc ctggccaccc gataatgagc cgccacgcct
      841 tacgcctctc cgatctcctt cgcgcgcagc gccacgggtc gagcggccgc ctcgcgtcga
      901 cccgccagtg cccaggcgcc cccatatccc cacgagcgaa gtcgtgaaac gtcccgcacg
      961 tccaattccg ccaacccggc cggtgaaaga cacagaaaag accggccagg atgccgaacc
     1021 cggaaagccg tgatcccggt tacagattcc aggcccgcgc gaaggcttca aacgagcggt
     1081 cgtaggtctt ttcagcctcg ggtggaaaac agcagccggg caactcccgg ctcctgaggt
     1141 gcgattggat gcactcgcag cacagggcat gattcgaaca tcccgggtac gaacacgtgc
     1201 aaaaggccat gtttttctca tgattcgggc acgatttggc catagaagca atcctcctga
     1261 gacagttcac tcagtataaa gagatccgtc gtcaatgcgg aactctgttg ttggcatcct
     1321 gtagttgaca tcctggcttc caagaacgcg ccgagcttct ccaaattcgt ggataattgc
     1381 ggcatactgt gtacaggcac tatacacacg ccggttttca ggcgcgagga gcggcgtgcg
     1441 aagcgggagc cgggttgtcg tatgcaggga ctgaatgagg tatatttttg ccggcatgag
     1501 ccaacacgaa aaggagaaaa gagatgaaac gaaccgcgct ggttgctgca tgtctcacca
     1561 tggccctaat cgtgggctgt ggtggcaaat cggcggagaa agcctccgaa gctctggccg
     1621 agcgaatgat cgaacgggcc gcacgggaaa gcggaggcaa catcgatgtg gatatttctt
     1681 caggaaagat cgccgtcaaa ggtgtcgatg aacacggcga agcggtcaac ttcactgtgg
     1741 ataccgatac cggcaccatg acgtctgaca atggcgccgt cagcttcgcg accggcgaag
     1801 ccgcgaaaat ccccgacgat ttccccaagg acgtccccct gtataaaggc gcccagctgg
     1861 tgagcgtgca tcgggacgcc aacgccggca tgacctccct tgccgctacc agtggcgacc
     1921 ccgcctccac cgtcgccgag ttctacaaaa aacaggcgga ggccaacggc tggacccagg
     1981 agactgtctt cagccagagc gattccatgc acatgatgtc atacgaaaag gacagccggg
     2041 agttgagcgt tgtcattgcg ggcgaaggcg gcgctacgca gattcaactg acccttgccc
     2101 agcagtagtg actcgctcaa tgcggctttg gctcatcata cggcacagcg cgccagcggc
     2161 cgccggtggc ggggccactc tgcccgtgcg ccggcaggga ctcctggcgg gcgctccggc
     2221 acgggttgat gtgccttcgc ggggtggctg gattggccga atcccatccc gctgtcggct
     2281 cgtaacgacg tcctcgtgtt tcagaccgaa ccccttccag aggcgctgga ggtgacgggc
     2341 gagctggagg tggaattgtg gatctcgtca accgctcccg ataccgattt caccgcaaaa
     2401 ctcatcgatg tatacccatc ttccgctgat tatccggacg ggttcgacct gatcatcggc
     2461 gacggcataa tccgcacccg ttttcgtgaa tcgctcacgg aggaggtctt catggagccc
     2521 ggcgcggtct atccggtacg catcacgctg tatccaacct ccaacgtgtt caagaaaggc
     2581 catcgcatcc gtctcgacat ctccagcagc aatttcccgc gttttgacct caatccgaac
     2641 accggcgagc cgctgaacca gaaccggcgc gtgcaggccg ccgtcaacag cgttgagcac
     2701 aacgccgccc attcatcgcg catctggctg cccatcgtgc cgccggatgc cgcgaaaccg
     2761 gcggattgat gccgggagta cccggatcgc gcccaattac gggccaccgg gccaaaatct
     2821 tgctgccggc cgttccgcag cccccctgcc gcgaattctc ttcgcggcct gcgccatact
     2881 ggaacgtgac ccgtccaggt tcaagtcact ccaaacggtc ttctttcttg acaacacctg
     2941 gcttaaaact caccaaatac gaagagcaaa atccagaaac cgtgcactta gtattagaat
     3001 gggctggtgt tacattgatt tagagacggt caaggtgtta tttacattac cagtgcacaa
     3061 tgtagaacgg cgggaaaggt aagggatgcg ccagtgcgcg cggccccaaa ccgaccggga
     3121 aggtcacgaa attccgtcaa ttcatcacat tctggcagcg agaaccggcg gtactctcca
     3181 gtgagagcga tcccacaccg gacgggttga aaatactgag cgttttggaa atatcagcac
     3241 tgctggcatg acactcggct ggaaagggag gctgttgcaa tacctttgct gaggcagtgt
     3301 tctttttgcc acagaagggg tcaatttaca cgacagaagg ggggccgcca tgaaatgccc
     3361 attttgtgca gaaaatatct cggatgacgc cattaagtgc cgcttttgcg gggaatttct
     3421 gcaggggcgg cctgcggccc tgtcggaaca gctcaaacac ccggcgccgg aggaattgat
     3481 ttggtccgga cgctcctcca agtgggccta tctcatccca tacctttgcc ttggcgtcgt
     3541 gctgctgcct gtttttggcg ttgggctggt cttctttccc atagccttcc acaagcattt
     3601 ccgaaagcgc tatgaggtaa ccaatcgtcg cattcgcgcg cgagtcggcc tgcttacaac
     3661 ctcggtgcac gaggtggcca ttcgggatat tcgttacgtg catatacatc gctcattcaa
     3721 agacaaatgc atcggcactg cgtcacttca atttgcctca gctggacatg ccggcatcga
     3781 agtgtctttc atttccatcg aaaaatggcg cacgattgcc gattatatcg aggactgcag
     3841 ggccatcgct tcgcactgat gaacacgctg tattatggcg acaatctcct tgtgctctga
     3901 gagtacattc ccggcgaatg cgtggatctg ggctgcctcg ccccgccttt caaaacgaag
     3961 aggtagccga ggcctgccaa aagatggttg aggaggaggg ggggatctct ccaaatttac
     4021 aggcgtttcg caccccggcc tgttgacatc atccgcattc cggtggcatc gtgtaattgc
     4081 tctggggggc ccggcataat tgacgataca acgtggaacg gatcacgggg aagtgtacgg
     4141 gccgcgactt ccgcctcgcg ccggagcaaa tgtaccgatg gaacacgcaa caacgtgtcg
     4201 aactccaagg gtctccacag gaggcgtcgc atgcggcaaa cacccttact cgacgaacat
     4261 aaggcactcg gtgggaaggt catcgatttc agcggctggg ctctgccggt tcagttcgcc
     4321 ggcattctcc aggagcacac gcatacccgt tccagtgcat ccattttcga ttgctcccac
     4381 atgggcgagt tccgcatcaa gggccgggag gccatcgagg cctacgatgc gttgattaca
     4441 accgataccg gccgtatccg gcccgggcgt gcccgctatg gcgccctgct taccggggac
     4501 ggcggctaca ttgacgacct catcaccatg cggctgggcg gcgatgagct gttcgtagtc
     4561 accaacgcgg gttcgctcga ccgggtcgcc gccgtgtttg cgtcgcaggt gccggggctg
     4621 gtcgacgagt ccgccgaaac ggccaagatc gacatccagg ggccgctcag ccgcgagatc
     4681 cttgtgtcta tcgggctgac tgccgc
//
LOCUS       DAOPBQ010000276         4701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15177_length_4701_cov_2.817477, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000276 DAOPBQ010000000
VERSION     DAOPBQ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15177_length_4701_cov_2.817477"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PKW60_09470"
     CDS             <1..840
                     /locus_tag="PKW60_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99700.1"
                     /translation="WPAAGVNMQGKGYISVPDVWNVPGRVDDIWAVPFGPEIYPEYLT
                     DINIWFCPSRANINADKYIGNNGDWVINGQLDPVLFEDDVSYSYYGYVMSNVHEYATM
                     MIAIDAATGMWSTGNTKTANQILDILRGDVDLTKVGVTRDQIRARIQNRIECYRTTPS
                     FYWPLGSSSPLANQFNIVGAGGGDTILKLREGVERFFVTDINNPAGSSKAQSTIPVMW
                     DEAMSGSGDSPDVLEKWKYNHVPGGSNVLYMDGHVEFLRWPTGDLDAIPMGQWSGMMG
                     SLW"
     gene            1052..3388
                     /locus_tag="PKW60_09475"
     CDS             1052..3388
                     /locus_tag="PKW60_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99701.1"
                     /translation="MPQSTAAQPPSGWVIVIPPDAATPVAKAAEMLSEEIYKRTGEVL
                     EVAAAMPAPEVQAIVLGSIDAPPAGMPPPLEDRAVPLEEEGYAVWTDSSSRPAATICV
                     LGREPRGAVFGAGAILRAARMVPGEFSLDPGFQVSTAPAYPVRGHELGYRHTSNTYDA
                     WSVEQYEQYIRDLAVFGANGVQLIVQLDSLETDGPHMAEPVLERTEKLTRILGDYGMR
                     VWVWMPPSEDAVTPESIPAVLEKCRAFFERCSPLDAIFVPGGDPGDTPPEILLPLLKE
                     MAAVLRESHPDAGIWFSNEDMSPEWNATLFEMLSRDQPDWLTGVVFGTWVKMPLTAMR
                     ARVPEQFPLVQYCDITHCIECQYPVPDWDRAFAFTLGREPINPRPVATAHIFDVMAPH
                     TIGFNTYSDGVNDDVNKFIWTAKGWNPQADVLHVLTEYGRYFVGEELGGKVAAGLLAL
                     EENWKGPLLSNAAVEKTLALWQDIEQQAGEAAETNWRLQMGLLRAYYDAYLYRKLVAE
                     TEREQRACAELARVPEVDVATAIANATVILSEADEDPDAPALRARVDELGGWLFKSIG
                     MQLSVDKYGAKNWERGAILDALDMPLNDRLWYEAEFREILALADEAARVARLHAIVNW
                     EDPGPGGFYDDLGNAWKQPHLVHQKDWASDPGAVESPQCEVIEVKGREAWRHSWLDQG
                     QTLFGTPLRMRYEGLDPQASYTLRVVYTGRFRPVMRLEANGTYELHGPMPQPEAPTPL
                     EFAIPQEATAGGTLELEWQHLSGRGCQVAEVWLLRTDG"
     gene            3507..>4701
                     /locus_tag="PKW60_09480"
     CDS             3507..>4701
                     /locus_tag="PKW60_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99702.1"
                     /translation="MKPSCEMHVISNTHWDREWSANFQETRMRLVEFLDALLEILDTE
                     PGYRSFLLDSQVVPIEDYLEVRPECRDRIVKHVTGGRLLIGPWYTCPEEFCVNGESLV
                     RNLWYGHRVARALGQVMKVGYSPFSYGQTSQMPQIYAGFGIDTILFYHGVTHGEAPNE
                     FIFEGPDGTRAFASQISGGARYNFYHKVYRKVVFPYEKDDRLYTWDQGGLPFHFCTED
                     TCEEQHTLLEPVPHFDKERLAHCVRELRDEERRVATTRYLAFMMGHDSSVPDAVELRL
                     IEEAQDATGQDALLHSSLPEWVAKVKAAAKDLPILKGERRTPKVRTGRVHLYSDVLSS
                     RARMKRFNARTEIALQRWAEPFSTMAWCLGREYPSSLLDLAWTTLLKCHAHDSIAGSG
                     VDDIEL"
BASE COUNT          936 a         1372 c         1492 g          901 t
ORIGIN      
        1 tggccggccg ctggcgtgaa catgcaaggc aaaggctaca tctctgtccc ggatgtgtgg
       61 aatgttcccg ggcgcgtcga tgatatctgg gcagtgccct tcggcccgga aatttacccc
      121 gagtatttga cggacatcaa catctggttc tgcccgtccc gcgcaaacat taacgcggac
      181 aaatacattg gcaacaacgg cgactgggtg atcaacggtc agctggatcc tgtgctattc
      241 gaggacgacg tgtcgtactc gtattacggt tacgtgatgt cgaacgtgca tgaatacgcc
      301 acgatgatga ttgcgattga cgctgcgacg ggcatgtggt ccaccggcaa cacgaaaacg
      361 gccaatcaga tcctggacat actcagaggc gacgtcgatc tgactaaggt gggcgttacc
      421 cgcgaccaga tccgggccag gatccagaac cggatcgaat gttatcgcac gacgcccagc
      481 ttctactggc cgctgggttc ctcaagcccc ttggcgaatc agttcaatat tgttggcgct
      541 ggcggtggag acactatcct caaactgcgg gaaggtgtgg aacggttctt cgtgaccgac
      601 atcaacaatc cggccggcag ttccaaggct cagagcacca tcccggtcat gtgggatgag
      661 gccatgtccg gttcaggtga ctctccggat gtcctggaga aatggaaata caaccacgtt
      721 cccggcggct ccaacgtgct ttacatggac ggccatgtcg aattcctccg ctggcccaca
      781 ggcgacctgg atgccattcc gatggggcag tggtcgggca tgatgggcag cttgtggtaa
      841 accctggtgc cacctgaggc gccgtgattg aacacggttt gcatggggcg ctgtctggag
      901 gtctgggcag cgccctttct cttgctccat aggcagtaca tcggttcagc gagcacggaa
      961 acggaggtcc tcggccatgc atcgcagcac aggttggttt ctcattgtct cggtggcttt
     1021 gttgtgtggg ggctgcggtg gtggaggcga gatgcctcag tctacggctg cacagccgcc
     1081 ttcgggctgg gtcatcgtga tcccgccaga cgctgccacc cctgtggcca aagccgcgga
     1141 aatgctcagc gaagagatct ataaacgcac gggagaggtg cttgaggtag cggccgccat
     1201 gccagctccc gaggtccagg cgattgttct cggcagtatc gacgcgccgc cagccggaat
     1261 gccgccccct ctcgaagatc gtgccgtgcc tctggaagag gaaggctatg ccgtctggac
     1321 ggacagttcg tcacgtccgg cggccaccat ctgcgtgctg gggcgggaac cccgcggagc
     1381 tgtctttggg gcaggggcca tcctgcgggc agcccgcatg gtaccggggg aattttcgct
     1441 ggatccgggc tttcaggtga gcacggcccc tgcatatccc gtgcgcggcc atgaattggg
     1501 ctaccggcac acctccaaca cctacgacgc ctggagtgtc gaacaatatg agcagtatat
     1561 ccgcgatctg gcggtattcg gcgccaacgg ggtccagctc atcgtccaac ttgattctct
     1621 ggaaacggac gggccgcaca tggccgagcc ggtcctggaa cggacggaaa aactgacccg
     1681 gatactcggc gattacggaa tgcgcgtctg ggtatggatg ccgccctcgg aagacgcggt
     1741 cacccccgaa tccatcccgg ctgtgctcga aaagtgccgc gcgttcttcg agcgctgcag
     1801 cccgctggac gcgatctttg tgcccggtgg cgatccgggt gacaccccgc ctgaaatcct
     1861 ccttccgctg ctgaaagaga tggcggcggt tctgcgcgag tcacacccgg atgcggggat
     1921 ctggttttca aacgaagaca tgtcgcccga atggaatgcc accctgtttg agatgctttc
     1981 cagggatcag ccggactggc tcacgggggt cgtgttcggg acctgggtca agatgccgct
     2041 gacggcgatg cgcgcccggg tgccggagca gtttccgctc gtgcagtact gcgacatcac
     2101 ccattgcatc gaatgccagt atccggtgcc ggactgggat cgtgcctttg ccttcaccct
     2161 gggccgggaa ccgatcaacc cgcgtcccgt ggccacggcg catattttcg atgtgatggc
     2221 gcctcatacc atcggattca atacctattc agatggcgtg aacgatgatg tgaacaagtt
     2281 tatctggacc gccaagggct ggaacccgca ggccgatgtg ctgcatgtgt tgacggaata
     2341 cggccgctac ttcgttggtg aagagcttgg tggcaaagtg gctgcgggtc tgctggccct
     2401 ggaagagaat tggaaaggcc cgctcctctc caatgcggcg gttgaaaaga cgctggcact
     2461 ctggcaggac atcgagcagc aagccggcga agcggctgaa acgaactggc gattgcagat
     2521 gggactcctt cgcgcctact acgacgccta tctctaccgc aaactcgttg ccgaaaccga
     2581 acgggaacag cgggcgtgcg cggaacttgc ccgtgtgccg gaggtcgacg tggccacggc
     2641 catcgcaaac gccaccgtca tcctgtcgga agccgatgaa gacccggatg cgcctgcact
     2701 tcgggcacgc gtggatgagc tgggcgggtg gctgttcaag agtatcggca tgcagctcag
     2761 cgtcgacaaa tatggtgcaa agaactggga acgtggcgcc atcctggatg cgctggacat
     2821 gccactgaac gaccggctct ggtatgaggc tgaattcagg gagatcctcg ccctggccga
     2881 cgaggcggcg cgcgtggcgc gtctgcacgc catcgtgaac tgggaagatc cgggtccggg
     2941 cgggttctac gacgatctcg ggaatgcctg gaaacagccc catctcgtcc accagaaaga
     3001 ctgggcatcc gacccggggg cggtggaatc gccgcaatgt gaagtcatcg aagtcaaggg
     3061 gcgggaagca tggaggcatt cctggctgga tcagggccag acgctttttg gcacgccgct
     3121 gcgcatgcgc tatgaaggcc ttgatcccca agcctcctac acgctccgcg tcgtctatac
     3181 cggccgtttc cggcccgtca tgcggctgga ggcaaacgga acgtacgaac tccacggacc
     3241 catgccgcag cccgaggcgc ccacgccgct cgaattcgct attccccagg aagcgacggc
     3301 cggggggacg ctcgagcttg aatggcagca tctttccggg cgaggatgtc aggtggctga
     3361 ggtctggctg cttcgtacgg acggctgaga gagaggtttc agagacagta tagtcctgac
     3421 ggccgcggcc cgttcgcgcc acatcgcgat ggccgcggcc gctcaggctt tcaatcgggc
     3481 agaatccggc aaggggtaga ggcatcatga aaccatcctg tgaaatgcat gtcatctcca
     3541 atacccactg ggatcgcgaa tggagcgcaa actttcagga aacgcgcatg cggctcgtgg
     3601 agtttttgga tgcgctcctg gaaatcctgg acaccgagcc gggttaccga tcttttctgc
     3661 tggactccca ggtggtgccg attgaggatt acctggaagt gcgaccggag tgccgcgacc
     3721 ggattgtgaa gcacgtaact ggcgggcggc ttctgatcgg cccctggtat acctgtccgg
     3781 aagaattctg cgtaaacggg gagtcgctgg tgcggaactt gtggtacggc caccgcgtcg
     3841 cgcgcgcact gggtcaggtg atgaaggtgg gatacagccc cttctcatac ggccaaacct
     3901 cacagatgcc gcagatctat gcggggtttg gcatcgatac catcctgttt taccacggcg
     3961 tcacccacgg cgaagcgccc aatgagttca tctttgaagg ccccgatgga actcgcgcgt
     4021 ttgcctccca aatcagcggc ggggcgcgct acaacttcta tcacaaggtc taccggaagg
     4081 tcgtctttcc atacgagaag gatgaccgcc tgtatacctg ggaccagggc ggcctgcctt
     4141 tccatttctg cacagaagac acctgcgaag aacagcacac gctgctggag cccgtgcctc
     4201 attttgacaa agagcgcctg gcgcactgtg tacgcgaact acgggatgaa gagcgccggg
     4261 tggccacaac acgctacctc gcgttcatga tgggccacga cagcagtgtg cccgacgcgg
     4321 tggagctgcg ccttatcgaa gaagcgcagg atgcgacagg gcaggacgcc ctgctccaca
     4381 gcagcctgcc ggaatgggta gcgaaggtga aagcggccgc gaaagacctg cccatattga
     4441 aaggcgagcg ccgcacgcct aaagtccgta ccgggcgcgt gcatctgtac agcgatgtgc
     4501 tgtccagccg ggcgcggatg aagcgcttca acgcccgcac ggagatcgcg ttgcaacgct
     4561 gggccgaacc tttttccacg atggcatggt gtctgggccg ggagtacccg tcgtcgctgc
     4621 tggacctggc atggacaacg ctgctgaaat gccatgccca cgacagcatc gcgggcagcg
     4681 gggtcgacga catcgagctg g
//
LOCUS       DAOPBQ010000277         4700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15183_length_4700_cov_3.050807, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000277 DAOPBQ010000000
VERSION     DAOPBQ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15183_length_4700_cov_3.050807"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..727)
                     /locus_tag="PKW60_09485"
     CDS             complement(269..727)
                     /locus_tag="PKW60_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99703.1"
                     /translation="MSPAALSGPGTAVRSGAEPLRAIGSVFGPITRVRGVAGTPELVL
                     GAVRSRLRPGAFIGPRTERGVSVEVLAEASSAPETARLVRVMRSGLEPEGSTRFPGAL
                     LMAAEVIGLAVRSTATLRSRASRDDAVIGVRSIRSPVRSTELKLLRGMLL"
     gene            complement(785..1081)
                     /locus_tag="PKW60_09490"
     CDS             complement(785..1081)
                     /locus_tag="PKW60_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99704.1"
                     /translation="MDRVERGGELGAGVLLKLRGSAVGAEDGPPMVTERVGAALRGVV
                     RKVSAGLDCCDGGDVTGVLMLRVRAPGISVAVLRGAGAIVGVRVLMLCSGGEML"
     gene            complement(1097..1306)
                     /locus_tag="PKW60_09495"
     CDS             complement(1097..1306)
                     /locus_tag="PKW60_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99705.1"
                     /translation="MPGTGLENDGALRIGLGAGLETEGALRMGLGAGLENDGALRMGL
                     GAGLDIEGMLRVLGLGAFLGMLGLL"
     gene            1411..2298
                     /locus_tag="PKW60_09500"
     CDS             1411..2298
                     /locus_tag="PKW60_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99706.1"
                     /translation="MRHRLGTACRVLLIFAAALTVTGCPYFPPNDGKSDYDIGFDDGF
                     ARNDWYWAGHDDGFDTIDWTIYYEGDTIPYIDALTYDAGFWDGVWWAYNDGYFVDYRY
                     AFIIGFSEGYDNAFYPDYLAFLASDVHIEYLHGGWIDGYNDGFSEGRVFGANDYEQGL
                     PFDWRSALFDYEDGVDLYFEEVDVGTGVYGPVYLYEYGTDPHTLKAYAPRGDGILGPE
                     RSIRKDLTKADDMDSRQLYRPLTADARQRYKVTPEKSMRADRKLQLKTTWLQRIQSYL
                     SAAKSAEIRSERVRQVSND"
     gene            2513..3088
                     /locus_tag="PKW60_09505"
     CDS             2513..3088
                     /locus_tag="PKW60_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015136850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPJ99707.1"
                     /translation="MALTASTMMALGTRAPDFSLPDTDGNVVALKNFEDQRVLLVMFI
                     CNHCPYVKHVRDELAQLSRDYAPASVGMVAINANDVEAYPQDGVEAMRAEKQTAGYLF
                     PYLFDESQATAKAYGAACTPDFFLFDSDRKLVYRGRLDDARPGNGLPVTGRDLRAALD
                     AALAGQPVEGDQKPSMGCNIKWKPGNAPVYG"
     gene            complement(3103..4092)
                     /gene="thiL"
                     /locus_tag="PKW60_09510"
     CDS             complement(3103..4092)
                     /gene="thiL"
                     /locus_tag="PKW60_09510"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPJ99708.1"
                     /translation="MSTVRDIGEFGLIDRIARLLPTSPAVVLGVGDDCAVLRFCERMM
                     LVSCDLFVENVHFRQDYAQPEQIGWKAAAAAMSDIAAMGGQPMFVLVALGCPPETPAQ
                     YVQMLYRGLSSAVSRFGAAIVGGDTSMSPDGIALDVTVIGQGIGNRCLTRRGARAGDA
                     LAVTGPLGLAAAGLHSLQHGHLAPALQAAHLSPRPRVPEGQWLCSQPFAHAMIDISDG
                     LAQDAKHIADASKIGVLIDTAKLPIHADLQAYCDQHGLDPLRFILAGGDDYELAAAVD
                     GEHAAKAVEAFGNEFRTGLSLIGEFTTEWTGVRVDGQPLDETGYSHFSSGPQA"
     gene            complement(4089..>4700)
                     /locus_tag="PKW60_09515"
     CDS             complement(4089..>4700)
                     /locus_tag="PKW60_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPJ99709.1"
                     /translation="GLDAALEFAAARDAVVLGPGCARHPDTREFVLNFIRRCPVPLVV
                     DADGLNNLSTDVAVLTQTESPVVLTPHPGEMARLAGKLPLEVQADRAGTAAGFAAKYG
                     CTVVLKGAGTLIATPGLDMHVNTTGNHGMGTGGTGDVLSGVIGGLIAQGLDCADAAIL
                     GVYAHGLAGDTAAKRYGCRGMIAGDLVEALPEAWLLLEQERKI"
BASE COUNT          887 a         1589 c         1408 g          816 t
ORIGIN      
        1 tgtacgccag ctcgtactgc gtggcggatc gcctgctcta cggtccttcc tacgtcgggg
       61 cctgctaccc cggcattatc cgcccggggt acggcggcga tgtatatatc tggaacatca
      121 atatcaacac cggctccagc agcacgaacc gctggtaccg gggcggaacc actatcccgg
      181 tgcgggatta ctccccgcgc cgtgtcatcc gcgggccgga caccatcggc ccccgcagcg
      241 tcgctgcatc gacgcgcagt gccgaacttc agaggagcat tccgcggagc agcttcaact
      301 ctgtcgaccg cacgggcgac cgtatgcttc gcactccgat gaccgcatcg tcgcgtgagg
      361 cccggctgcg gagcgttgcc gtggatcgca ccgccaatcc aatcacttcc gccgccatca
      421 acagtgcgcc cggaaatcgc gtggagccct caggttccag cccggaacgc atcacgcgca
      481 ccaatcgggc tgtctcggga gccgaggagg cttcggccag gacctccacg gacacaccgc
      541 gttcggttcg cggcccaatg aatgcccctg gcctcaatct cgaccgtaca gcgcccagca
      601 ccagttcggg cgtccccgca acgccgcgca cccgggttat cggcccgaac acggagccaa
      661 tcgcccgcag cggttccgct cccgagcgca ccgccgtacc cggtccggac agagccgccg
      721 gggacatcgg agaggctcct cgcgcgcgca ctatcgacaa ttcctacggg cgcgcgcgga
      781 cccctcagag catctcccca ccggaacaca gcatcagcac gcgcacgccg acaatggcgc
      841 cggcgccgcg cagcactgcg acggatatcc ctggggcgcg cacccggagc atcaatacac
      901 ctgtaacgtc tccaccgtcg cagcaatcca gcccggcgga cactttgcgg acaacgcccc
      961 ggagcgccgc gcctacccgt tcggtaacca tcggcggccc atcttcagcg ccgactgcag
     1021 acccgcgcag cttcaggagc acgccggcgc ccagttctcc accgcgctcc accctgtcca
     1081 cgccgccagc accgtcctac aacagtccca gcattccgag aaacgctccg agcccgagca
     1141 cacggagcat gccttcgatg tcgagtccgg cgcccagccc catgcgcagc gccccgtcat
     1201 tctcgagccc ggcgcccagc cccatgcgca gcgctccttc ggtttcgaga ccggcgccca
     1261 gccctatgcg aagcgctccg tcattctcga gtccggtgcc cggcacgatg cgcagtgccc
     1321 cctcgggcgg cgcttcacgg tcattctctc cgcgcggccg gtgacaacgg tactaacaag
     1381 gaggtggagg tatctgccat ggaaaacacc atgagacata gactgggaac cgcgtgccgg
     1441 gtgctgctga tcttcgccgc cgcactcacc gtgacaggtt gcccttactt cccgcccaat
     1501 gacgggaaaa gcgattacga tatcggtttt gatgacggct tcgcccgcaa tgactggtac
     1561 tgggcgggcc acgacgatgg ctttgacacg attgactgga ccatctacta cgagggcgac
     1621 accattccat acattgacgc tctgacgtat gacgccgggt tctgggatgg cgtctggtgg
     1681 gcgtacaacg acggttattt cgtcgattac cggtacgcct tcatcatcgg gttcagtgaa
     1741 ggctacgaca acgcgtttta cccggactac ctcgctttcc tggccagcga cgtgcacatc
     1801 gagtatctgc acggcggatg gatagatggg tacaacgatg gtttctcaga aggccgcgtt
     1861 ttcggcgcca atgattacga acagggcttg cccttcgact ggagaagtgc cctgttcgat
     1921 tatgaggatg gcgtggatct ctactttgag gaagttgatg tcggcacggg cgtgtacggt
     1981 ccggtctatc tttatgaata cggaacggac ccgcacacgc ttaaagctta cgcaccacgc
     2041 ggcgacggca ttctgggccc tgaacgcagc attcgcaaag atctgacgaa ggcggacgac
     2101 atggacagcc ggcagctcta ccgcccactc acagcggacg cgcgacagcg ctacaaggtg
     2161 actccagaga agagcatgcg cgctgatcga aagctccagc ttaaaacgac atggcttcag
     2221 cgcattcaaa gctacctgtc ggccgcgaaa tcggccgaga tcaggtccga acgggtccgc
     2281 caagtcagca atgactaggc catggcctgt ttctcctcca ccccaaaggc aaggccccgg
     2341 gaatcatccg gggccttgcc ggtttcagac cggcagcctc tggatctgcc cgtccggatt
     2401 tccgtatcct tgaaaccggg ggaggggaga gttcttcctc ggaatatgga ccgctgagcg
     2461 cacgtttacc gttgagatcg gctctgaggc gagatttaag gaggacaggc ccatggcatt
     2521 gacggcgtcc acgatgatgg ctctcgggac acgcgccccg gatttttctc tccccgacac
     2581 agacgggaac gtggtagcac tcaagaattt cgaggatcaa cgcgtgctgc tcgtgatgtt
     2641 catctgcaac cattgcccct acgtgaagca cgtgcgggat gagttggcgc agcttagccg
     2701 cgattacgcg cccgcgagtg tgggcatggt ggccatcaac gccaacgatg ttgaggcgta
     2761 tcctcaggac ggcgtcgagg cgatgcgggc cgaaaagcaa acggcaggct acctgttccc
     2821 gtaccttttt gatgagagcc aggcgaccgc caaggcctat ggcgcggcgt gcacgccgga
     2881 tttcttcctg ttcgacagcg accgcaagct ggtctaccgc ggccggcttg acgacgcccg
     2941 cccgggcaat gggctgccgg tcacagggcg tgatctccgc gctgcactcg acgcagcgct
     3001 ggcggggcag ccggttgagg gcgaccagaa accaagcatg ggatgcaata tcaaatggaa
     3061 gcccggcaac gcacccgtct atggctgacc acgcgttgaa gttcaggcct gcggcccgct
     3121 ggagaaatgg ctgtagccgg tctcgtccag gggctgcccg tccacccgga cgccggtcca
     3181 ttcggtcgtg aactccccga tcaggctcag cccggtgcgg aattcgtttc cgaacgcctc
     3241 cacggccttg gcggcgtgct cgccatccac cgcggcggcc agttcatagt cgtccccgcc
     3301 cgccaggata aatcgcagcg gatccaggcc gtgctggtca caataggcct gcagatcggc
     3361 gtgtatgggc agtttcgctg tgtcaatgag gaccccaatc ttgctcgcgt ctgcaatatg
     3421 cttcgcatcc tgggccaggc cgtcactgat gtcgatcatc gcgtgtgcga aaggctgact
     3481 gcacagccac tggccttcgg ggacgcgtgg ccgcggggaa agatgcgcag cctgaagcgc
     3541 aggggcgagg tggccgtgct gcagggaatg gagaccggcc gccgcaaggc ccaacggccc
     3601 cgttactgcc agggcgtcgc ccgcgcgggc gcctcgccgc gtcaagcacc ggttgccgat
     3661 cccctggccg attacggtca cgtcaagcgc gatgccgtcc gggctcatcg acgtgtcgcc
     3721 accgacaatc gcggcgccaa aacgggacac ggccgacgac aagccgcggt acagcatttg
     3781 cacatactgc gccggcgtct cgggcgggca ccccagggct accagcacaa acatcggctg
     3841 gccgcccatg gcggcgatgt cgctcatggc agcggctgcg gctttccatc cgatctgttc
     3901 cggctgggca tagtcttgcc tgaaatgcac gttttccacg aagaggtcgc acgagaccag
     3961 catcatgcgc tcgcagaaac gcagcacggc gcaatcgtcc cccacgccca gcacgacggc
     4021 cggcgatgtg ggcagcaatc gcgcgatgcg gtcgatcaac ccgaactcgc ctatgtcgcg
     4081 caccgtgctc atattttccg ttcctgctcc agaagcagcc aggcctcggg gagtgcttcc
     4141 acaagatccc cggcaatcat cccgcggcat ccgtaccgtt tcgcggcggt atcgcctgcg
     4201 aggccgtgcg catagacgcc cagaatcgcg gcatccgcac agtccagtcc ctgagcaatc
     4261 aatccgccga tcactcccga caacacatcg ccggtcccgc ccgttcccat gccatgattt
     4321 ccggttgtat tcacatgcat atccaatccc ggagtggcaa taagggttcc cgcgcctttg
     4381 aggacgaccg tacagccgta ttttgccgca aatcccgccg ccgtccccgc gcgatctgcc
     4441 tgaacttcga ggggtaattt gcctgccagc cgggccatct ctccagggtg gggtgtgagg
     4501 actaccggcg attccgtctg ggtcagcacg gcaacgtccg tcgagaggtt gttgagcccg
     4561 tccgcgtcca caaccagcgg cacggggcag cgccggatga agttcaggac gaactctctc
     4621 gtgtccgggt gccgcgcaca gccgggcccg aggacgacgg cgtcccgggc cgcggcgaat
     4681 tccagagcgg catcgagacc
//
LOCUS       DAOPBQ010000278         4695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15193_length_4695_cov_3.942026, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000278 DAOPBQ010000000
VERSION     DAOPBQ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15193_length_4695_cov_3.942026"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="PKW60_09520"
     CDS             complement(<1..1741)
                     /locus_tag="PKW60_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99710.1"
                     /translation="MRPWVRNTLEHFICLLAVAAGALAVLYPVAVDGRIPMPAGDALF
                     ASPWEEARPAGLEETGQPSPAAARQHYPWYAFLSDTASRGDSLLWNPLEGCGTPFFAL
                     WRSRCLSPFSIPFCFSPPERALPVSAFLKLLVAGSCAFIAGRRLGFASPLALFIGFSF
                     QLSAPFFYWLDSPLSDTLPWLPLLMIFVDRLALSRIHYWPYGALVLALMLLGGEPGAV
                     GGMFLVTLLFILARNLLGQRGYAQLFASTSTYLIVLVTALALCGIQIVPFYELVQSCV
                     SSGNLEPAVDFRMNHLAALVLPHLFGRSPYAFTDDLLCAQARIALLLHAGIIQLSLLP
                     LWFAVRRYSPAPQRHRIEALLIASASGLVLALLVWVPLAGVFPFKYLRPEHLLAAGSL
                     FVAVAAAAAAEEWLLLDADGCKEALKRLVVFLPVFLVLALILGALGWQAAPTPNLTLL
                     GQAAIPLLLFILTVALVFVTMLRPNLRLMGYALSVLAAVQALVVLHPGSGYPAFLAKE
                     ELFPRTPFIDALQDDGYRAGGSLRLKEWPLTGNLIPQTYAPGGVILKRHQAFFAAAER
                     QPLLMRRAGSRNFV"
     gene            complement(1758..3158)
                     /gene="lpdA"
                     /locus_tag="PKW60_09525"
     CDS             complement(1758..3158)
                     /gene="lpdA"
                     /locus_tag="PKW60_09525"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPJ99711.1"
                     /translation="MIACDVAVLGAGPGGYVAAIRAAQRGAKTVVVECDQVGGVCLNR
                     GCIPTKTLIHTAGLYQKMQEAERFGLKVKGLSVDMDGLLKHKDNVIKTNRDGIKALFK
                     AHGIELIKARGVVAKPGVVKAGKEEIHARAIIVATGSAPARIPGLETDGERIIGSTEA
                     LELAEVPKRVVVIGAGAVGAEFACIWNGLGAEVTLVEMMPSVLPIEDEELTKRLGTLL
                     KKRGINVFTGTTVKSMTRKGNTVALELSGAKEDSIKAEYVLVGIGVRYNSSPVTDEPS
                     LGVQVTPKGAIVVDSRMETNIPGIFAIGDVTGRTMLAHGASAEAIVAAENATGGNRII
                     DYRVVPSCTFTHPEVASVGLTEKEAAATGASIKVGRFSYRASGRAHAMGETEGLVKIV
                     GDAATGEVLGVHILGAEAGELIAAAAMAMQLEATAETIADTIFTHPTLSETIKEAAED
                     FLGIGIHTPPKAVKRG"
     gene            complement(3174..4520)
                     /locus_tag="PKW60_09530"
     CDS             complement(3174..4520)
                     /locus_tag="PKW60_09530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HPJ99712.1"
                     /translation="MHEIRLPQLGQSVEEASIVEWRKKEGDPVKTGEVLFTVQTDKAE
                     IECESTADGILRKILVVPDVEVAVMTVVALVGEADEPLPDLAQYSEAAPETAPAPSAS
                     SPAPAPPSAPATPAVQAPAEAPAAPAQEQAPGGRTPVSPRARKRAEQLKVDPAFAQGS
                     GAGGRVMEADVEVYAAQVALTPTARQLVLDNALDATRIKGTGPGGKITKADVQQALAS
                     PRAAASPMVPGEVQRVPLTPMRRIIAERMAASKFSAPHYYITVEVDMAAAMAFRRGCT
                     EFKPSVNDLVLRAAALAIQQHPQVNARWAGDAIELAGDINLGFAVAMPAGLIVPVVRQ
                     AQTKTLKDISRECSGLAEKARAGKLLPDDYAGNTFTVSNLGAFGVDQFTAIINQPDSA
                     ILAVGQIKGRPVVIEGGIYVRPIMKMTLSSDHRVIDGAVAAQFMKTLKGILEDANF"
BASE COUNT          888 a         1486 c         1483 g          838 t
ORIGIN      
        1 gcacgaagtt gcgcgagccc gcccggcgca tcagcagcgg ttggcgttct gccgcggcga
       61 agaaggcctg gtggcgtttc agtatgacgc cgcctggcgc gtaggtctga gggatcaggt
      121 ttcccgtcaa cggccattct ttcaaacgga ggcttccgcc cgcgcggtag ccgtcgtcct
      181 gaagtgcgtc gatgaaaggc gtccgcggga agagttcttc tttcgcgagg aatgccggat
      241 acccgctgcc cgggtgaagc acgacgagcg cctgcaccgc ggccaggacc gacagcgcat
      301 atcccatgag cctcagattg ggcctcagca tggtcacaaa aaccagcgcg acggtcagaa
      361 taaacaagag cagcggaatt gcggcctgcc ccaatagagt aaggttaggc gtgggtgcgg
      421 cttgccagcc cagcgcaccg aggatgagcg ccagcacaag aaagaccgga aggaacacga
      481 ccaggcgttt gagggcctct ttacagccgt ccgcgtcgag caggagccat tcttcggctg
      541 cggccgccgc cgcgacggcc acgaagaggc tgccagcggc caggaggtgt tccggacgca
      601 ggtatttgaa cggaaacacg ccagccagcg gtacccagac cagcagcgcc agcacaagtc
      661 cgctggctga ggcaatcaac agggcctcga tccggtggcg ttgcggtgca ggcgagtaac
      721 ggcgcacggc aaaccatagc ggcagaaggg atagctgaat gatgccggcg tgcagcagca
      781 gcgcaattcg ggcctgggcg cacagaaggt cgtccgtgaa cgcatacggc gaacggccga
      841 agagatgcgg caggaccagg gccgccagat ggttcatcct gaagtcaacg gcgggttcga
      901 ggttgccgga agatacacag gattgcacca gttcgtaaaa cggcacgatc tgaatgccgc
      961 agagggccag tgccgtaacg agaacgatga gataggtgct ggtgctggca aacagctgag
     1021 cgtacccgcg ctgtccgagc agattgcggg cgaggatgaa cagcagggtg accaggaaca
     1081 tgccgccgac agcaccgggt tcgccgccaa gaagcatcag cgccagcacg agggcgccat
     1141 acggccagta atggatccgg ctcagggcca ggcgatccac gaagatcatc aaaagaggca
     1201 accacggcag cgtgtcggaa agcggcgagt caagccagta aaagaacggg gcgctcagtt
     1261 gaaatgaaaa gccgatgaac aacgccagcg gtgaggcaaa tcccaggcgc cgccccgcga
     1321 tgaatgcgca cgaacccgcc accagcagct tcagaaaggc cgataccggg agcgccctct
     1381 ccggcggcga gaaacagaac gggatggaga acggcgagag gcagcgcgag cgccacaagg
     1441 caaaaaacgg cgtcccacaa ccctccagcg gattccacaa cagcgaatca ccgcgtgagg
     1501 ccgtgtccga caggaaggcg taccacggat aatgctggcg tgccgcggca ggggagggct
     1561 ggccggtctc ttccaggccc gccggacggg cctcctccca gggcgaggcg aacagcgcgt
     1621 cgcccgcggg catgggaatg cggccatcga cggcaaccgg atacaagacc gccagcgccc
     1681 cggccgctac ggccaggaga caaataaaat gttccagcgt atttcgaacc cagggtcgca
     1741 cgggcggcct cccggattta tcctcgtttc acagctttgg gcggcgtgtg aatgcctatc
     1801 ccaagaaaat cttccgcggc ttccttaatg gtctcggaaa gcgtgggatg cgtaaaaatc
     1861 gtgtcggcga tcgtctcggc tgtcgcctcc agctgcatgg ccatggccgc ggccgcgatc
     1921 aattcgccgg cttccgcacc caggatgtgg acgccgagga cctcgcccgt tgccgcatcg
     1981 ccgacgattt tcaccaggcc ctcggtctcg cccatggcat gcgcgcgtcc gctcgcccgg
     2041 taactgaagc gtccgacctt gatggatgcg cccgtcgcgg cggcttcttt ctcggtgagc
     2101 cccacgctgg cgacttccgg atgcgtgaac gtgcaggaag gcaccacgcg gtagtcgatg
     2161 attcgattgc ctcccgtggc attttcagcc gcgacgatgg cttccgcgga agccccatgg
     2221 gccagcatgg tcctgccggt gacgtcgccg atcgcaaaaa tgcccggaat gttggtttcc
     2281 atgcgcgaat ccaccacaat cgcgcccttg ggagtcacct gaaccccgag agaaggctcg
     2341 tcggtcacgg gcgacgaatt gtagcggacg ccaatgccta cgagcacgta ttccgccttg
     2401 atggagtctt ctttcgcgcc ggaaagctcc aaagcgaccg tattgccttt gcgcgtcatg
     2461 gatttcacgg tcgttccggt aaatacgttg ataccgcgct ttttgagcag ggtgcccagc
     2521 cgtttggtca attcctcgtc ctcgatcggc aggacgctgg gcatcatctc gaccagcgtg
     2581 acttcagcgc ctaatccgtt ccagatgcag gcaaattccg cgccgacggc gccggcgccg
     2641 atgacaacga cccgtttcgg cacttcagcc agctcgaggg cttcggtgct gccgatgatg
     2701 cgttcgccgt ctgtctcgag cccgggaatg cgcgcgggag cactgcccgt cgcgacaata
     2761 atggcccgcg cgtgtatctc ttccttgccc gccttgacga cgccgggctt cgccaccaca
     2821 ccccgggcct tgatcaattc aatgccgtgc gctttgaaca acgccttgat gccgtcccgg
     2881 ttggtcttga tgacgttgtc tttatgtttc agcaggccat ccatgtcgac cgaaaggccc
     2941 ttgaccttca gcccgaagcg ctcggcttcc tgcatttttt ggtaaagccc tgcggtgtgg
     3001 atgagcgtct tggtggggat gcagccgcgg ttcaggcaca cgccgccgac ctggtcgcac
     3061 tcgaccacca cggtcttcgc cccgcgctgc gcagcgcgga tcgctgccac ataacctccg
     3121 gggccagcgc caagtacggc cacatcacat gcgatcattt ctgattttcc cccttagaag
     3181 ttcgcgtctt cgaggatgcc cttaagcgtc ttcatgaatt gggccgctac ggcgccgtcg
     3241 atcacccgat ggtcgctcga cagcgtcatt ttcatgatcg gccgtacgta gatcccgccc
     3301 tcgatcacta ccggccggcc cttgatctgt ccgaccgcca ggatcgcgct gtcgggctgg
     3361 ttgataatcg ccgtgaactg gtcgacgcca aacgcgccca ggttggaaac cgtgaaggtg
     3421 ttgcctgcgt agtcgtcggg cagcaatttg ccggcgcggg ccttctcggc cagcccgctg
     3481 cactcccggc tgatgtcttt gagcgttttg gtctgcgcct gccgcacgac gggcacgatc
     3541 aggccggctg gcatcgccac cgcgaagccc agattgatgt cgcccgccag ttcaatggcg
     3601 tcgcccgccc agcgcgcatt cacctgggga tgctgctgga tggccagggc ggctgcgcgc
     3661 agcaccagat cgttgaccga gggcttgaat tctgtgcaac cccgccggaa ggccatggcc
     3721 gccgccatgt cgacctcgac cgtgatgtaa tagtgcgggg cgctgaactt gctcgccgcc
     3781 atgcgttccg cgatgatgcg gcgcatcggg gtgagaggca cccgctgcac ttcgccggga
     3841 accatcggag aagcggccgc gcgcggggag gccaaagcct gttgcacgtc ggctttggtg
     3901 atcttgccgc cggggcccgt gcctttgatg cgcgtggcat ccagcgcgtt atccaggacc
     3961 agttggcgcg cggtcggtgt aagcgccacc tgggctgcgt aaacctcgac gtcggcttcc
     4021 ataacccgtc cgcccgcacc cgaaccctgc gcgaaggccg ggtcgacctt gagctgttcc
     4081 gcgcgctttc gcgcccgtgg cgagacaggt gtacgcccgc ccggagcctg ttcctgtgct
     4141 ggcgccgcgg gtgcttcggc aggcgcctga acggccggcg ttgccggggc ggatggtgga
     4201 gcgggggcgg gggaggaggc gctgggtgca ggcgctgttt ccggtgccgc ttcgctgtat
     4261 tgggcaagat ccggcagtgg ctcatccgct tcgccaacca gtgcgaccac ggtcatgacc
     4321 gccacttcaa cgtccggaac tacgaggatt ttgcgcaaga tcccgtccgc cgtggattca
     4381 cactcaatct cggccttgtc ggtctgcacc gtgaacagca cctcgccggt cttgacgggg
     4441 tcgccttcct ttttacgcca ttcgacgata gaggcctctt caacggactg cccaagctgt
     4501 ggaagtctaa tttcgtgcat tacgcgtcct gtccaagcca accctcctga cggaggcgtt
     4561 tgccggcttc gtaaatgagc cgcgcgaact ccgatccgga ttccagttca caaattttct
     4621 gcagaacggc cgcgattcca ccctgttcac gaatggattg aagccggcct gccgccgcat
     4681 cggcgggatg atcgt
//
LOCUS       DAOPBQ010000279         4680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15266_length_4680_cov_4.157189, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000279 DAOPBQ010000000
VERSION     DAOPBQ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15266_length_4680_cov_4.157189"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKW60_09535"
     CDS             complement(<1..302)
                     /locus_tag="PKW60_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99713.1"
                     /translation="MLRNKNHTVSRRTFVAAAGAAVAAPYIIPASALGKEGVAAPSER
                     IVLGVIGTGGQARGLTGNAIKQPDTQIVALCDVNAKTVAEAKSQVDEFYGNQDCWT"
     gene            complement(454..1428)
                     /locus_tag="PKW60_09540"
     CDS             complement(454..1428)
                     /locus_tag="PKW60_09540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPJ99714.1"
                     /translation="MQAETSRQSVSVVCPVFNECENLPELYEQLRAALDITALPWEIL
                     FVDDGSTDGSNEVIQRLHEGDSRVRGILLSRNFGHEAAATAGIELAGGDAVILMDADL
                     QDPPSLIPALVEKWHEGYDIVCAQRTARHQDSAFKRTSAYLFYRAMTLLVGWNLPADT
                     GNFRLMNRAAVEAFRNCPERNRFVRALVAWTGFRQTTVPFERPPRQAGKSKYRTWQMF
                     ALALTSITSFSVAPLRIATAIGLLVVPFATLTVLGILVGRLFGAPVPVNVVVVASIWF
                     FGGLQCLLVGIVGEYVGRIYIEAQHRPLYIIRRHLGEANAGIVRRRAE"
     gene            complement(1483..2601)
                     /locus_tag="PKW60_09545"
     CDS             complement(1483..2601)
                     /locus_tag="PKW60_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPJ99715.1"
                     /translation="MSAVKRVRVAMIMAGGSGERFWPLSRRLRPKQLLGLTSPTRTLL
                     EEAVSRIAPVVASRDVFIVTGRHLVAPIRKAETGVPRENVIAEPCKRNTSGALAYAAA
                     CMLSRYGTSPENITMAVTTADHAIGDPALFQETVAAVLDLVEQENALATLGIVPTRPE
                     TGFGYIQIAPGAEPLIDGKIAIYPVTAFHEKPQRETAEEFVASGRYFWNSGMFFWRLS
                     SFLEELDRVRPMLSDAVRRMTEAMNAGDDLGVHQIFDSLEDISIDFALMEHARRVVVA
                     RAEFPWDDVGAWSTLDRTLAHDEQGNVCVGEPVLVDCENCIVYNESGGEKMAVGIIGC
                     SDMIVVATSDGVLVAPKERAQEVRRIVAALKDRGAEQI"
     gene            2885..>4680
                     /gene="ftsH"
                     /locus_tag="PKW60_09550"
     CDS             2885..>4680
                     /gene="ftsH"
                     /locus_tag="PKW60_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPJ99716.1"
                     /translation="MNGFLKQASLWIVLIIIVVLALTQLSKSQKPGEKLGRLDFVEQI
                     EKGNVDAVVVKDLGDSLYEVRAKLKQPYKNSDTIVFQWKDFPTDWDEKLTKQGIVPMM
                     ERDNNLWLGMLVNVVPFILIVGLFWFFMFRQMQGGSNKALSFGKSRARLMNHSDKVVT
                     FDDVAGVDEAKEDLQEIIAFLKDPKKFSRLGGRIPRGVLLVGPPGAGKTLLARAVAGE
                     AHVPFFSISGSDFVEMFVGVGASRVRDLFQQGQKHAPCIIFIDEIDAVGRQRGAGLGG
                     GHDEREQTLNQLLVEMDGFNTSEGVILMAATNRPDVLDHALLRPGRFDRQVVVSNPDI
                     MGREAILRIHVRNNNVPLDDEVDIRTFARGTPGFSGADLANLVNEAALLAARRDHDKV
                     QIADFEEAKDRVLMGPERRSLVISPAEKWCTAVHEAGHALVGRLIPHGDPIHKVTIIP
                     RGKALGVTSMLPEEDRHSLSKSYCQAQICYMMGGRAAEEIALKELTSGVSNDLKRATE
                     LAHAMVCQWGMSDLGPISFGSNSEVFLGRDLVRERDFSEETASAVDKAIHAILDEAYV
                     RARDLLTKHKLLLLAVSVALVKRETLEGDELD"
BASE COUNT          920 a         1368 c         1458 g          934 t
ORIGIN      
        1 caggtccagc agtcctggtt accgtagaat tcgtcgacct gacttttggc ttcggcgacg
       61 gttttggcgt tgacgtcgca cagggccacg atctgggtat ccggttgttt gatggcattg
      121 cccgtgaggc cgcgcgcctg cccgcccgtg ccgatgacgc ccaggacgat gcgttcgctg
      181 ggggcggcca ctccttcttt ccccagggcg gatgcgggga tgatgtacgg agccgcgacg
      241 gctgcgcctg cagccgcgac gaacgttcgc cgcgatacgg tgtggttctt gttccgcagc
      301 atgagatctc tcctccgttt cgagctgatc agacagccgg gcagcggctg cccactgggt
      361 gaaatccgga aatatactcc gaaaccaaac tacttcatcg tagaaggccc atcaaggcgg
      421 attcaaggaa aaaggcaccc tgcgttttcc ggttcactct gcccggcggc ggacgatgcc
      481 ggcatttgcc tccccgagat gtctgcggat gatgtaaaga ggccgatgct gggcttcgat
      541 atagatgcgg ccgacatatt ctcccacaat gccaacgagc aggcactgca gaccgccgaa
      601 gaaccagatg ctcgccacga cgacgacatt tacgggcacc ggtgcgccaa acagccttcc
      661 gaccaggatt cccagcacgg tcaaggtcgc gaacggcacc accagcagcc cgatcgccgt
      721 ggcgatccgc aggggcgcca ccgaaaagct tgtgatgctg gtcagggcga gggcgaacat
      781 ctgccaggtc cggtatttgc tcttcccggc ctgccttggc ggacgctcga acggcaccgt
      841 ggtctgtcgg aagcccgtcc aggcgaccag cgcgcgcaca aagcggttgc gttccgggca
      901 gttccggaag gcttccacgg cggcgcggtt catcagacgg aaattgcccg tgtcggccgg
      961 gaggttccat ccgacgagga gcgtcatggc gcggtaaaac aggtaggcag aggtgcgctt
     1021 gaaggcggaa tcctggtggc gcgccgtgcg ctgggcgcag acaatgtcgt agccttcgtg
     1081 ccatttttcg accagggcgg ggattagcga aggggggtcc tgcaggtccg cgtccatgag
     1141 aatcaccgca tcaccgccgg caagctcaat acctgcggta gccgctgctt cgtgcccgaa
     1201 attccgcgac agcaagattc cacggacacg tgagtctcct tcgtgaagcc tctggatcac
     1261 ctcgttcgac ccgtctgtgc tgccatcgtc tacgaagaga atctcccacg ggagcgcggt
     1321 tatatccaag gcggcacgga gctgctcgta cagttccggc aggttttcgc attcgttgaa
     1381 caccgggcag accacggaca cgctctgccg actggtttct gcctgcatgg ccccctcttg
     1441 gatttggttc cgccgcccga taccgcggcg atgcggcttg cactagatct gttctgcgcc
     1501 gcgatccttc aacgcagcta cgatccggcg gacctcctgt gcccgctcct tcggcgcgac
     1561 cagcacgccg tccgacgtcg ccaccacgat catatccgag catccgatga tgcccaccgc
     1621 catcttttcg ccgcccgatt cgttgtaaac gatacaattc tcacagtcga ccagcacggg
     1681 ctcgcctaca cacacattgc cctgctcatc gtgcgccaga gtgcggtcga gcgtggacca
     1741 tgcgcccaca tcatcccacg ggaattcggc gcgcgccacc acgacccgcc gcgcgtgttc
     1801 catgagcgca aaatcaatcg aaatgtcttc cagagagtcg aaaatctggt gcacgccgag
     1861 gtcgtcaccc gcattcattg cctcggtcat tcggcgaaca gcatccgaga gcattgggcg
     1921 gacccggtcg agctcttcga gaaaactcga caggcgccag aaaaacatgc cgctgttcca
     1981 gaaatagcgg cccgaagcga cgaattcttc cgcggtctcg cgctggggct tttcatggaa
     2041 ggcggttacc gggtatatgg cgatctttcc atcgatcaac ggctcggctc ccggcgcaat
     2101 ctgaatatat ccaaagccgg tttcggggcg ggttggcaca atgccgagcg tcgccagcgc
     2161 gttttcctgt tccacgaggt ccagaacggc ggcaacagtc tcctggaaaa gcgccgggtc
     2221 tccgatggcg tggtccgccg ttgtgacggc catggtgatg ttttcgggag aggtgccgta
     2281 gcgcgacagc atgcaggcag ctgcataggc cagcgctccc gaggtgtttc gtttgcatgg
     2341 ttccgcgata acattttccc ggggcactcc cgtttcagct ttccggatcg gggcgaccag
     2401 gtggcgccca gtgacgataa acacgtctcg cgaggccacc acgggcgcaa ttcgcgagac
     2461 cgcttcctca agcagggttc gggtgggcga ggtcaagccg agcagttgtt tcggccggag
     2521 tctccgggag agcggccaga accgctcgcc gctacctccg gccatgatca tggcaacccg
     2581 gacacgcttg acagcactca tttatgggct ccttgggttc gtggaaccat cttacgccag
     2641 gcgcgcgcgg ggggcaactt gagctctgcg gagtggggcg ccggttctgg gcgcgtgaat
     2701 aaatcctgcg ccggagcgtt tcatctaagg tagaagaaat cggcgccgaa acccgtaaca
     2761 cggtaatttg gcaggttttg cgccgtgcgg gaattgtatg cgctgcagcc gtgtgttatg
     2821 attaggctgt gcgtgtgcgc ggtgcgctgc gcctcagatg taacataagg atgctcagtc
     2881 agttatgaac ggttttctca agcaggcctc actttggatc gtcctgatca tcatcgtggt
     2941 gcttgctttg actcagcttt caaagtcgca gaaacccggc gagaagctgg ggcgcctcga
     3001 ttttgtcgag caaattgaaa aaggcaatgt ggacgccgtc gtcgtgaaag acctcggcga
     3061 cagcctgtat gaagtccgcg caaagctcaa acagccctac aagaattccg acacgatagt
     3121 gtttcaatgg aaggacttcc caacagactg ggacgagaag ctgaccaagc aggggattgt
     3181 cccgatgatg gagcgggaca ataatctctg gctggggatg ctggtcaatg tggtcccctt
     3241 cattttgatt gtcggtctgt tctggttttt catgttccgc cagatgcagg gcgggagcaa
     3301 taaagccctc tcgtttggaa agagccgggc gcgcctcatg aaccacagcg acaaggtggt
     3361 gacgtttgac gatgtggccg gagtggatga ggcgaaagaa gaccttcagg agataatcgc
     3421 gttcctgaag gatcccaaga aattttcacg gctcggcggc cgtattccca ggggtgtgct
     3481 gctcgtgggg cctcccggag cgggcaagac cctgctggcg cgtgccgtgg cgggtgaagc
     3541 ccacgtgccg tttttcagca tcagcggttc ggatttcgtc gagatgtttg tgggcgtggg
     3601 cgcgagccgc gtgcgagacc tgtttcaaca ggggcagaaa cacgccccgt gcatcatttt
     3661 tatcgacgag atcgatgccg tcgggcgcca gcgtggcgcg ggcctgggcg gcggccacga
     3721 tgaacgcgaa cagactttga accagttgct ggtggagatg gacgggttca atacgagcga
     3781 aggcgtcatt ttgatggccg cgaccaatcg ccccgacgtg ctggaccatg cgctgttgcg
     3841 cccgggacgc ttcgaccggc aggtggtggt gagtaaccct gatattatgg gccgggaagc
     3901 cattcttcgc attcacgtgc gcaacaacaa cgtgccattg gacgatgaag tcgatattcg
     3961 cacctttgcc cgcggcaccc cggggttttc gggagccgac ctggcaaatc tcgtcaatga
     4021 ggccgcttta ctggcggcgc gccgcgatca cgacaaagtg caaatcgccg atttcgaaga
     4081 agccaaagat cgcgtgctga tggggccgga acggcggagc ctggtcatca gcccggcaga
     4141 gaaatggtgt acggcagtgc atgaagcggg ccatgccctc gtgggccggc ttattccgca
     4201 cggagacccc atccacaaag taacgatcat cccgcgcggc aaggccctcg gcgttaccag
     4261 catgctgccg gaagaggatc ggcattcgtt gtcgaaatcg tactgccagg cgcagatctg
     4321 ttacatgatg ggcgggcgtg ccgcggaaga aattgcgctg aaggagctca ccagtggcgt
     4381 gtcaaacgac cttaaacgcg ccacggaatt ggctcacgcc atggtgtgcc aatggggcat
     4441 gagtgacctg ggccccattt cgtttggctc caactcggag gtctttctcg gccgcgatct
     4501 cgtgcgggag cgcgatttca gtgaagagac ggcatcggcg gtcgacaagg ccattcacgc
     4561 gattctggac gaggcctatg tgcgagcccg ggaccttctt acgaaacaca agctcctcct
     4621 gctggctgtc tccgtggcgc tcgtgaaacg cgagacactg gaaggcgatg agctggatga
//
LOCUS       DAOPBQ010000280         4672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15294_length_4672_cov_4.403942, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000280 DAOPBQ010000000
VERSION     DAOPBQ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15294_length_4672_cov_4.403942"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="PKW60_09555"
     CDS             <1..1624
                     /locus_tag="PKW60_09555"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPJ99717.1"
                     /translation="GGGAHAGVLDQHARMRQTAQIARGITERSLALIQRHINNAEGHP
                     DDVLITAFNASAFPRTEVLTAVLDVPPCAASREFDLIAADGKTRVPVQVVTRRPHYAT
                     MNHAGDAAHMMSCEQVKFHFEARGLPALGYSTFRFNPKGRFTRGSLICGSNALENEHL
                     HVQIHDNGTLSIRHKASGMEFDGLHYFEDSGEAGQAWMHVEPGSDKVINSRGFPVSVS
                     LEEDGPLLARYRIEHFLKIPSALGNAGSNAWERLDGFDNSARRSAETTDLNIISVVTL
                     RKGARSVEVQTRFHNAAENHRLRVVFPTRLSRAKNCWAESAFDVVERPIVPGPGHPWH
                     GIGKRTFPMQRFIDVSDGKAGLAILNDGLREYEVTQDPERAIAVTLLRAFEVSICTSA
                     AGWDVRPGMKLSQSPGEHEYRYVIYPHVGRWDEAEVLAESELLAAPVRLAQAGRHGGT
                     LPKNMSFFQLTPSNLVLSAMHRSSSDDSLLIRLFNPTEKIVTGTLTFFKPVRAAELVT
                     LEEAPVRKLKPEGKKLALRVGSRKIITLKIAL"
     gene            1865..>4672
                     /locus_tag="PKW60_09560"
     CDS             1865..>4672
                     /locus_tag="PKW60_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPJ99718.1"
                     /translation="MAIQGMKNLNTLYLRKIKSRVAEIRARFLYSERTPIDDVSMAET
                     PKHLTITEARRLPYKPVAEGQRWGSDWGTAWFRLRISVPRSMRGETVMLLFDLENGEG
                     LIFRDGEPVQGLCWSRKEYVLLGKARGGEQIELFVEAGANARLGAFVERAVHLPHIAV
                     LNHEVWDAFWDISALADIVDPELHKDWTGKPYHLPFDGDTLLARIVFALNEAVDLFDY
                     REPSREELRAQAGRVRRRLKPIYDCKATDAAQTFAAMGHAHIDVAWKWPLAETVRKCG
                     RTFANVLDLMERYDDFVFAQSQPQLYAYAAERYPSLYKRVREMVKRKRWIPTGCMWVE
                     ADCNITGGESLVRQILFGTRFFQQEFQYRAETLWLPDVFGFSAALPQIMKRSGIKYFF
                     TTKLALNQFMKFPYHTFHWEGLDGSRVLSHFMPGEEYSAELEPWLIRTAVHDYAEKDR
                     CSLQVMPFGHGDGGGGPARAHLERLRRFADFEAMPRLVSMPPDTFFAELERESRKLPV
                     WRGELYLQNHRGTYTSQAQVKKNNRQAELLLRDTELLAALAMAEGAGYDQDGLADAWR
                     LVLLNQFHDILPGSSIDKVYDESERQFVTVFSKVGAIRDTAFQRLAGHVDTQGAGEPV
                     LAFNALSWARRDLLEVEAHTLDTGKTWEAAGSNGEISPVQVGEDGKARFVGSVPSIGC
                     KVFHIRPCDALPPEVAVSERSLENGLIRLRFDARGRLRSVYDKRHRREVLASGATGNQ
                     FILFEDKMASCGASWDIDIFYNDKPLEFDGKLLSVEVAERGPVRSVLRFNRAIGKSRI
                     TQDVILSAESPRIDFQTTVEWGDEHDVMLKTAFPVNVRSETARYETQFGNLERPTHWN
                     LPYDFARFEAPAHKWVDLSEGNYGVALLNNCKYGYDIRDNVMRITLLRAPKDPGESTD
                     VNRTHTFTYALLPHEGDFTHGV"
BASE COUNT          963 a         1385 c         1421 g          903 t
ORIGIN      
        1 gggcggcggg gcgcatgcgg gcgtgctgga ccagcatgcc cgtatgcgcc agaccgccca
       61 aatcgcccgt ggaataacag agcggagtct cgccctcatt cagcgccata tcaacaatgc
      121 agaggggcat cccgacgatg tgctgattac agccttcaac gcctcggctt ttccgcgcac
      181 ggaagttctg accgccgtgc tggatgtgcc cccatgcgcc gcctcgcgcg aatttgacct
      241 gatcgcggcc gatggcaaga cgcgcgtgcc ggtgcaggtc gtcacgcggc gcccgcatta
      301 cgccacaatg aaccacgccg gcgatgccgc gcacatgatg tcgtgcgagc aggtgaaatt
      361 ccatttcgaa gcacgcggcc tgcccgctct gggctattcc acatttcgtt tcaatccgaa
      421 agggcgattc acgcgcggga gcctgatctg cggcagcaat gcgctggaaa acgaacacct
      481 gcatgttcag attcacgaca acggcacgtt gagcatccgg cacaaggcct ccgggatgga
      541 gttcgatggc ctgcactatt ttgaagacag cggcgaggcg ggccaggctt ggatgcatgt
      601 ggaaccaggt tcggacaagg ttatcaacag ccgcggattc cccgtgtcgg tttccctgga
      661 agaggacgga ccactcctgg cccggtaccg gatcgagcat tttctcaaaa ttccatcggc
      721 cctgggcaat gccggcagca acgcgtggga gcgcctggac ggctttgaca attccgcgcg
      781 acgaagcgcg gaaaccacgg atttgaatat catttcggta gtgaccctgc gaaagggcgc
      841 ccgctccgtc gaagtgcaga cacgttttca caatgcggcc gaaaaccacc ggcttcgtgt
      901 ggtgtttccg acacgcctca gccgggcaaa gaactgttgg gcggaaagcg cttttgatgt
      961 ggtcgaacgg ccaattgtgc cgggaccggg gcatccctgg catggcatcg gcaaacgaac
     1021 cttccccatg caacggttca ttgacgtcag cgacgggaag gcaggcctgg cgatcctgaa
     1081 tgatggactg cgggagtatg aggtcacgca agaccccgaa cgagcgattg ccgtgaccct
     1141 tctgcgggcc ttcgaagtgt cgatctgcac gtcggcggcg ggctgggatg tccggcccgg
     1201 catgaaattg tcgcagagtc ctggcgaaca cgagtaccgc tatgtgatct atccgcatgt
     1261 ggggcgctgg gacgaggcag aggtgcttgc ggagtctgaa cttctcgcgg cgccagtgag
     1321 acttgctcag gccggacgtc atggcggcac acttccgaaa aacatgagct tttttcagct
     1381 cacgccatcg aatcttgtgt tgagtgcgat gcaccgctcg tcttccgatg attcgctgct
     1441 gatccggttg tttaacccga cggaaaagat cgtcacggga accctgacgt tcttcaaacc
     1501 ggtgcgggct gccgaattgg ttaccctgga agaagcgccg gtgcgaaaac tcaaacccga
     1561 agggaaaaag ctcgcgctgc gcgtcggcag ccggaagatc atcacgctga aaatcgccct
     1621 gtaaccgctc cctccaaacg agaacacggc ggcgcgcaga gacggggaat ggcgtagctt
     1681 ccagtttttt ccgcgcgcag cctggccctg aaaatgctca actttcgagg gttttcagca
     1741 gcgcctgagc tggttgaagt cgacaggggg tctgcctatc atcgggatgc tggaagcgag
     1801 ccggagcctg ggttgagatt tctctcaacg atccgcatcg ctttactctg agacggaggt
     1861 gcatatggca atccaaggca tgaagaacct gaatacactg tatctgcgca aaattaaaag
     1921 ccgggtagct gaaattcgcg cccgcttcct ctattcggaa cggactccga tcgacgatgt
     1981 gtccatggcc gaaaccccga agcacctcac aattaccgag gcccggcgtc ttccctacaa
     2041 gcccgtggcc gaaggccagc gctggggcag cgattggggc acagcgtggt tccggttgcg
     2101 catctcggtg cctcgctcga tgcgcggtga aacggtgatg ttgctgttcg acctggagaa
     2161 cggcgaaggg ctgattttcc gcgatggcga gcccgtgcag gggttgtgct ggtcccgtaa
     2221 ggaatatgtg ctgctcggta aggcccgcgg aggggagcaa atcgagctgt ttgtggaagc
     2281 cggagcaaac gcccgcctgg gggcatttgt cgaacgggcc gtccatctgc cgcacattgc
     2341 cgtgctcaat cacgaggtct gggacgcctt ctgggatata tctgcgctgg ccgacatcgt
     2401 tgatcccgaa ctccacaaag actggacggg gaagccgtac cacctgccgt ttgacgggga
     2461 cacgctcctc gcccggattg tctttgcact caatgaggcc gtcgacctct tcgactaccg
     2521 ggagccgtca cgggaagagc tgcgggcgca ggcgggccgt gtgcggcggc gcctcaagcc
     2581 gatctatgac tgcaaggcca ccgacgccgc ccagaccttt gccgcgatgg gccacgccca
     2641 catcgacgtc gcctggaaat ggcccctggc ggagacggtg cgtaaatgcg ggcgcacctt
     2701 tgcaaacgtg ctcgacctga tggagcgcta cgatgatttc gtctttgcgc agagccagcc
     2761 ccagctctat gcctacgccg cggagcgcta cccttcgctc tacaaacgcg tgcgtgagat
     2821 ggtcaaacgg aagcggtgga tccccacggg atgcatgtgg gtggaggccg actgcaatat
     2881 caccggcggc gaaagcctcg tccgccagat tctctttggc acgcgctttt ttcaacagga
     2941 atttcaatat cgtgcggaaa cgctctggct cccggatgtc ttcggatttt ctgcggcgct
     3001 gccgcaaatc atgaagcgca gcgggatcaa atactttttc accacaaaac tcgccctgaa
     3061 tcaattcatg aaatttccct atcacacctt ccactgggaa ggactcgatg gcagccgggt
     3121 actttcccat ttcatgccgg gggaagagta cagcgccgag ctggaaccct ggttgattcg
     3181 cacggcggtg catgattatg ccgagaagga ccggtgctcc ttgcaggtga tgccgtttgg
     3241 ccacggagat ggcggaggag ggccggcgcg cgcacacctc gaacggttac ggcgtttcgc
     3301 cgactttgaa gccatgccgc ggcttgtctc catgccgcca gacacctttt ttgcagaact
     3361 ggaacgcgaa agtcgaaaat tgccggtgtg gcggggggaa ctctaccttc agaaccaccg
     3421 ggggacctac acctctcagg cgcaggtgaa aaagaataac cggcaggctg aactcctcct
     3481 gcgtgatacc gagctgctgg cggctctggc catggcggag ggcgcaggtt atgaccagga
     3541 cggtctcgcg gatgcgtgga ggctggtcct cttgaaccag ttccacgata tcctgcccgg
     3601 gagctccatc gacaaggtct atgacgaatc cgagcggcaa ttcgtcaccg tgttttccaa
     3661 agtcggagct attcgcgaca cggcgttcca gcggcttgcc ggccacgttg atactcaggg
     3721 cgccggcgaa cctgtcctgg ccttcaacgc gctctcatgg gctcgccgcg acctactcga
     3781 agtcgaggcc cacacgctgg acacgggcaa gacctgggag gcggccggct cgaacggaga
     3841 aatctcccct gttcaggtgg gggaggacgg aaaagctcga tttgtgggca gcgtaccgtc
     3901 cattggctgc aaagtcttcc atatccgccc ctgcgatgcc cttccgcccg aagtcgcagt
     3961 gagcgagcgg tctctcgaaa acgggctcat ccggcttcgc tttgatgcgc gcggccggct
     4021 tcgcagcgtg tacgacaaac ggcaccggcg ggaagtgctt gcgtccggag cgactggcaa
     4081 ccagttcatc ttgtttgagg acaaaatggc gagctgcggc gcttcgtggg acatcgacat
     4141 cttttacaac gataaacctc tcgaattcga cggaaaactg ctttcggtgg aagtggccga
     4201 acgtggcccc gtgcgctccg tgctccgatt caaccgggcg ataggaaaat cccggatcac
     4261 ccaggacgtg atcctcagcg cagagtcccc gcgcattgat tttcaaacca cagtggagtg
     4321 gggcgatgag catgatgtca tgctcaaaac cgcattcccc gtgaacgtcc gctcggaaac
     4381 cgcccgttac gagacccagt tcggcaacct cgagcgcccg acacactgga acctcccata
     4441 cgacttcgcg cgcttcgaag cgcctgcgca caaatgggtg gatctatcgg aagggaacta
     4501 tggtgtggcc cttctcaata actgcaaata cggatacgac atccgggaca acgtgatgcg
     4561 aatcaccctg ctgcgtgcgc ctaaggaccc cggggagagc accgacgtca acagaacgca
     4621 cacgttcacc tatgcgctcc tgccgcacga gggcgatttc acccacggcg tg
//
LOCUS       DAOPBQ010000281         4663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15324_length_4663_cov_3.809245, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000281 DAOPBQ010000000
VERSION     DAOPBQ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15324_length_4663_cov_3.809245"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..561)
                     /locus_tag="PKW60_09565"
     CDS             complement(85..561)
                     /locus_tag="PKW60_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99719.1"
                     /translation="MKLRQATPGLLAIAVLITGCAQQMPEMPEIPFINPKLTDEDQVL
                     RVLDEVWRGMQSKRIYQIMSNVSRSYKDRDGRDYDALAAYLNTIFEEYRSIKIARAKP
                     RVLVQGNQARALETFGTIAKPEPRSEYPPIDLHGQVSVYLEKVDGHWFIVEWGSIQ"
     gene            complement(558..1100)
                     /locus_tag="PKW60_09570"
     CDS             complement(558..1100)
                     /locus_tag="PKW60_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99720.1"
                     /translation="MQPHFAGLSEKRGAATMKRALIVVLTGLLLASAGCQTNPFQRKP
                     QAPPADLQFETADGRVSSSPTVPSDGLPMATQQRFSDVPLPEKLKEDAERTFVYEGPG
                     LQIGRTVYTSRAKPPELAQFYVRECPTAGWKLENVVQADVITLNFTKPDKRLRVTISD
                     RGFVKGRELVLLIVPEKSSL"
     gene            complement(1299..2183)
                     /locus_tag="PKW60_09575"
     CDS             complement(1299..2183)
                     /locus_tag="PKW60_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPJ99721.1"
                     /translation="MRHLNRRDFLKWSATTAVAGLGIPALAQAPTPRLTAQTVRTLGT
                     TGIRCTLLGQGTGMRGWNGNSDMTRLGRNEFVQLLEHGHASGISFFDLADMYGSHAYL
                     REAMKRSIKREDIMILSKAISRSPALMKADIERFRHEIDTDYLDVLLMHCITEPDWTE
                     KPEYQASLEVMHEAKAKGKIRAVGCSCHNFDALRRAAASPHVDVILARYNPFSIKMDE
                     SVENVTAVLKEAREAGKGILGMKVVGEGQLTGEPDKISESVKFIVNSGLIDAITVGFT
                     EQAQIEDMIGRIDAATRV"
     gene            complement(2425..3789)
                     /locus_tag="PKW60_09580"
     CDS             complement(2425..3789)
                     /locus_tag="PKW60_09580"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HPJ99722.1"
                     /translation="MATAREFGAKGDGVADDTQALQRAIDESGVLVLEKGTYRVSAPL
                     LLDTTQLGYAGVRGAQGTSRIVMTGPGPAFRIVGDHQGTANPISFQEHTWERERFPVV
                     EGIEILGRHDEADGIELFRTMQCTIQNVLIRECRYGIHLVERNRNPIIANSHIYRCLD
                     TGIFFDSVNLHQAIIIGNHISYCARAGIRQFNGDVHNVQISGNDIEYNSGFTEGSSGE
                     VVLEVPDDGLISEYMITGNTLQATPDAPGANIIMSGRPLDDDSAVRAVSITGNVIGDR
                     DKCIVMQHAHRAVTIAANTIYSGTTVSVELTHCGQLAFTGNTVNHSPWPRGMSDIGGV
                     LLEDCMDCAITGNVLTGLAYGDGTRGGAIHLARCSGVAVSDCQFVSPLHRGVYLESCT
                     LCRIADNTFINRRDDHVMKAGVQIAGDSRNNLVQHNLLQAGSEGAIICEESHGVVLNN
                     TIMA"
     gene            complement(3886..>4663)
                     /locus_tag="PKW60_09585"
     CDS             complement(3886..>4663)
                     /locus_tag="PKW60_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99723.1"
                     /translation="PSQNAFTSVESDVLPFDVLPGPLQGAHALVLPYGADSLRLTQQP
                     GPDGESDATHFEAALSGDGTLALTVTYTANGNGQAMYRSLKDLDEQALRNQLTQLAAY
                     LHPSAVLESYDVSELSDISVPPVLTLHCRIPEYAVKAGDELLMFTLPAVSYSAHLVGR
                     PAREFGLFWDHVTRETTEGRITLPQGYALYSLPGNVTIDSGVAAYRARLTANDGTVAF
                     EDAYTLSVDEAPASAYAGYKQCLERRAGLARQRIILTRTK"
BASE COUNT          838 a         1408 c         1446 g          971 t
ORIGIN      
        1 agaatttctt ttacccggct gcccttttgt ttctttgcca tgaatgagga actttccgac
       61 aggttgtgag tccagagtta cgaactattg aatcgagccc cattcgacga tgaaccagtg
      121 gccgtccact ttctccagat agacgctgac ctgaccatgg aggtcaatgg ggggatattc
      181 cgaacggggc tccggtttcg cgatggtgcc gaacgtttcc agcgcgcgcg cctgattccc
      241 ctgcaccagg actcgcggtt ttgcgcgcgc aatcttgatg ctccggtatt cttcaaagat
      301 cgtgttgagg tatgccgcga gggcatcgta atcgcgtccg tcgcggtcct tgtagctccg
      361 ggagacgttg gacatgattt ggtaaatgcg ttttgactgc atgccgcgcc agacctcgtc
      421 caaaacgcgc aaaacctgat cttcatccgt gagtttcgga ttgatgaacg ggatctccgg
      481 catctcgggc atttgctggg cgcagcccgt gatgagcacc gcgatggcga gaaggcctgg
      541 cgttgcctgc cgtagtttca tagggacgac ttttcaggga cgatcaacaa gaccagttcc
      601 cttcctttga caaagccccg atcagaaatc gtaacccgaa ggcgcttgtc tggtttcgtg
      661 aaattgaggg tgatcacatc cgcctgcacg acgttttcga gcttccagcc ggcggttggg
      721 cattcccgca cataaaactg cgccagttcg gggggcttgg cgcgcgaggt gtacaccgtg
      781 cggccaattt gcagccccgg cccttcgtag acgaacgtgc gttcggcgtc ttctttcagt
      841 ttttcaggca acggcacatc ggaaaaacgt tgctgtgtgg ccatcggcag cccgtccgac
      901 ggaacagtgg gggaggagga cacccggccg tccgcggtct cgaattgaag gtctgccggg
      961 ggcgcctgag gctttctctg gaacgggttg gtctggcagc ccgccgaggc caacaaaaga
     1021 cccgtcaaca ccacaattag cgcacgtttc atggttgctg cacccctctt ttctgaaagt
     1081 cctgcaaaat gcggctgcac gccgttctaa cgttccaggg ggaaatctgc cgaatcctgg
     1141 aaggatacac atcccccctc gcccatagga tagccccacc gggctcaaag ttccagcctg
     1201 aagcgataag ataccttccg tccggcgcca aatcaagatc gacacgagcc gcgcgaaaat
     1261 aaccccgggc ccgcccgtcc gcatcatcca gacaggtgtc agacgcgtgt ggccgcgtcg
     1321 atccggccga tcatgtcctc gatctgggcc tgttccgtaa aaccgacggt gatcgcgtcg
     1381 atgagcccgg aattcacgat gaacttgacg gattcgctga tcttgtccgg ctcgccggta
     1441 agctggcctt ccccaacgac tttcatgccg aggatgcctt tgccagcctc gcgcgcctct
     1501 ttcaaaaccg ccgtgacgtt ttcgacagac tcgtccattt taatgctgaa gggattgtag
     1561 cgggccagga tcacatccac gtgaggggag gccgcggccc tgcgcagcgc gtcgaaattg
     1621 tggcaggagc acccgacggc gcggatcttc cccttggctt tcgcttcatg catgacctca
     1681 agtgaggcct gatactccgg tttctccgtc cagtccggtt cggtaatgca gtgcatgagg
     1741 agcacatcca ggtagtctgt atcgatctcg tggcggaaac gttcgatgtc ggctttcatg
     1801 agtgcgggcg aacgcgagat ggccttggac aggatcatga tgtcttcgcg tttgatgctg
     1861 cgtttcatgg cttcgcgcag gtaggcgtgc gagccgtaca tgtctgcgag gtcaaagaag
     1921 ctgattccgc tcgcatggcc gtgttcgagc agctggacga attcgttgcg gcccaggcgg
     1981 gtcatgtcgg aattgccgtt ccagccccgc atacccgtgc cctgccccag cagtgtgcaa
     2041 cgaatgcccg tcgtacccag ggtgcggacg gtctgcgccg tgaggcgcgg agtcggggcc
     2101 tgagccagcg ccggtatgcc aagccccgct accgccgttg tggccgacca tttcagaaaa
     2161 tcgcgccgat tgaggtgtcg cataacgtcg atgctccttg ttccgctgct attgtacaca
     2221 atcaaagcgc acaacgggac tccccggcca cgcgaggtgc gtgtgtctgc cctgatccat
     2281 caggattgcg gaatcttgta ggggcaggcc cctgtgcctg cccttcttca gacgggtgcg
     2341 cgggggctat ccggatgggc agccacaggg ggccgtccct gcaatccgga ggataacggc
     2401 gccacgcccg ccagcgccgt ttttttatgc catgatcgta ttgttgagta cgacgccgtg
     2461 gctttcctcg cagatgatgg cgccctcgga gccggcctga aggaggttgt gctgcacgag
     2521 attgttgcgg ctgtcgccgg cgatttgcac accagccttc atgacgtggt cgtcacgccg
     2581 gttgatgaac gtgttgtcgg cgatgcggca cagcgtgcag gattcgagat agacaccgcg
     2641 gtgaagcggc gacacgaact ggcaatcgga taccgcgacc ccggaacagc gtgcgaggtg
     2701 tattgccccg ccgcgcgtgc catcgccgta ggccaggccg gtcaggacgt tcccggtgat
     2761 ggcgcagtcc atacaatcct cgagcaacac tccgccgata tcgctcatgc cccgcggcca
     2821 cggcgagtgg ttaaccgtgt tgccggtaaa ggccagctgt ccgcaatgcg tgagctcgac
     2881 gctgaccgtg gtgccgctgt agatggtgtt agccgcaatg gtgaccgcgc gatgcgcatg
     2941 ctgcatcacg atgcacttgt ctcgatcgcc gatgacgttg ccggtgatgc tcacagcccg
     3001 gaccgcggag tcgtcgtcca gagggcggcc cgacatgatg atgtttgcgc caggggcatc
     3061 gggcgtggcc tgaagcgtgt tgcccgtgat catgtattcg ctgatgaggc cgtcgtcagg
     3121 cacttccagg acaacctcgc cgctggaacc ctccgtgaac cccgagttgt attcgatgtc
     3181 gttgcccgag atttggacgt tgtgaacatc gccgttgaac tgccggatgc ccgcgcgggc
     3241 gcagtaactg atgtggttgc cgatgatgat cgcctggtgg aggttgacgg aatcaaagaa
     3301 gatccccgta tccaggcagc ggtagatgtg cgaattggcg atgatcgggt tgcggttgcg
     3361 ttcgaccaga tggatgccgt agcggcactc gcgtatgagc acgttctgaa tcgtgcattg
     3421 catggtacga aacaactcga tgccgtcggc ttcgtcgtga cggccgagaa tttcaatccc
     3481 ttccaccacc gggaagcgct cgcgctccca ggtatgctcc tggaaggaga tcgggtttgc
     3541 cgtgccctga tgatcgccca cgatccggaa ggccggcccc ggcccggtca tcacgattcg
     3601 agacgtcccc tgtgcgcctc gaacaccggc gtagccgagc tgggtcgtgt cgagcagcag
     3661 gggcgcggaa acccggtacg tccctttctc cagtaccagc acgccgctct cgtcaatcgc
     3721 gcgctgcaat gcctgcgtat cgtccgcgac accgtcgcct ttggccccga attcccgcgc
     3781 cgtcgccata attggtcctc cttctgccgg ccccgggccc atggtagcga cggtacgccc
     3841 caggtgcaac cgcgcgcggc cttctggcct tttccgctcc agcggctatt ttgtccgggt
     3901 caggatgatc cgttggcggg ccaggccggc ccggcgttcc aggcattgct tgtacccggc
     3961 ataggccgac gccggtgctt catcgacgct cagcgtgtag gcgtcctcaa aggccaccgt
     4021 gccgtcgttc gcggtcagcc gcgcccggta tgccgctacc ccggaatcaa tcgtgacgtt
     4081 tccgggcagc gagtagagcg cgtacccctg cggcagggtg atccggcctt ccgtggtctc
     4141 ccgggtcacg tggtcccaga agagcccgaa ttcacgcgcg gggcggccca ccaggtgggc
     4201 gctgtacgac accgcaggca aggtaaacat caggagctca tcgcccgcct tgaccgcgta
     4261 ttcggggata cggcagtgca aagtgagcac aggcgggacg gagatgtcgc tgagctccga
     4321 cacgtcgtag ctttccagca ccgcgctggg gtggagatag gccgcgagct gcgtcaattg
     4381 attgcgcaac gcctgttcgt cgagatcttt gaggctccgg tacattgcct ggccgtttcc
     4441 attggcggta tacgtcaccg tgagcgcgag tgtcccgtcg ccgctgagtg cggcctcaaa
     4501 gtgcgtggca tcggattcgc cgtccggccc gggctgctga gtcaggcgga gcgagtccgc
     4561 gccgtagggg agcacgaggg catgggcgcc ctgcaacggc cccggcagca catcaaaagg
     4621 cagtacgtcg ctttccaccg acgtgaatgc gttttgagag gga
//
LOCUS       DAOPBQ010000282         4648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15379_length_4648_cov_3.378184, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000282 DAOPBQ010000000
VERSION     DAOPBQ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15379_length_4648_cov_3.378184"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PKW60_09590"
     CDS             complement(<1..946)
                     /locus_tag="PKW60_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99724.1"
                     /translation="MRHRDALETAPLSRRGFIKATATSASGIVLAGVSAEALAAGPPW
                     QQTSDKKIRIGIVGGGFGLSFHWHEHPNCIVEAVSDLIPERRERLQKKYGCEKTYESL
                     EQLIQDPAIDAVGVFTEATNHARHVIDCMEHGKHVVSAVPACCSLEEAAAMKEAKERT
                     GLTYMMAETSHYRPETITARRLHQDGLFGELVYCEAEYYHPGIGWADNELSTRDGKRT
                     WRWGYPPMWYPTHSTAFLVSVTRERLVKVSCIGTRRPADPGFQDNPYNNPFANGLALF
                     LTDQRHPFRCHVCWDIHGHGERAQWFGTQAALYMPGCAG"
     gene            complement(974..2212)
                     /locus_tag="PKW60_09595"
     CDS             complement(974..2212)
                     /locus_tag="PKW60_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99725.1"
                     /translation="MHELNSKTPHPFSMSRRGFLASASASLALPLATSAIGQSLVDTP
                     VWEQRSDKKIRIGVVGGGFGQSFHWHEHPNCIVEAVSDLRPDRLTQLQGKYKCEKTYE
                     SLEKLILDPAIDAVAVFTEAPNHARHVVDCMEHGKHVISAVPACCTLDEAALLKEVKE
                     RTGLTYMMAETSSYRWETITARRLVQDGLLGELIYTEGEYYHPMDKAGRAALWYYNDQ
                     RTWRYAYPPMLYPTHSSAFLVGVTGERLVKVSCIGWGDAEHDTALRENAYDNPFTNGM
                     AMFLTDRGHPFRCNVAWDIKAHGERAQWFGMQGAMYMPGSGGQPFALQLPDQTLSGLP
                     DYWHMVPEAMRYDTGHGKSHPFLTNEFIMALVEEREPLVDLYEALAFTVPGIVAHESS
                     FRDGEQLPIPSFDPAGRARA"
     gene            complement(2420..2815)
                     /locus_tag="PKW60_09600"
     CDS             complement(2420..2815)
                     /locus_tag="PKW60_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPJ99726.1"
                     /translation="MKEIAVHTTSHTQFVNIDAKIESAVAATGIRDGICFVFVPHTTA
                     GVTINENADPDVTADMIQALERVVPWKAGYAHAEGNAAAHVKASMMGFSQAIPVRGGR
                     LAFGTWQSLYFCEFDGPRSRKVWVKVVPA"
     gene            3076..>4648
                     /locus_tag="PKW60_09605"
     CDS             3076..>4648
                     /locus_tag="PKW60_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020618977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPJ99727.1"
                     /translation="MPVRSPLRFASPRLSLQDLTGKDYIEAVCSADSFLSGTSATELR
                     RIAAQKVAFFPVSLQRALSSRLPQVGTECIPPAGATCTGATSVQFARNSTREQAPVSA
                     LGYYRIGEDGRLYLTSKSEHYHAPLGHRFPGYALLNHARALGIPNATHNNTRGHITRL
                     LEQEMVRAAAGIAPGDAQMLQRTLRSTSMNVLNRVLNLETGSLAAEAALKMILARFFR
                     SEAGSPTPPYAGRIPVVVVLGDEEGGLEANYHGTTFLTQTMRGMWPEVAALLEQSGAF
                     MVRAVRPNNLENLEAAFAQWDQPPYKIAGFFHELILMNYAAIRLSKAFLRRAYALCRQ
                     HDVPVVCDEIQSCVWSPDLFLFREYGLKPSFVVVGKGFPGGEYSASRVLFSAAMDSLP
                     QFGALVTNGQEELASLAYLITMRWVEANREHIETVGAYYEERLRELASRHAGVITAIE
                     GRRHLAGVFFDDVELSIQFAEQLSAGGLDISVQTYKKGCPPSALTKLPLIAGTDVVDV
                     IVARMEQALQALIKRG"
BASE COUNT          875 a         1385 c         1453 g          935 t
ORIGIN      
        1 gtccggcgca tcccggcata tacaaagccg cctgagtgcc gaaccactgg gcgcgctcgc
       61 cgtggccgtg gatatcccag cagacgtggc accggaaagg gtggcgctgg tccgtcagga
      121 acagggccag gccgttggcg aagggattgt tgtatggatt gtcctgaaaa ccgggatcgg
      181 cgggcctccg ggttccgatg cacgagactt taacgagacg ttcgcgggtg acgctgacga
      241 gaaacgcggt gctgtgggtc gggtaccaca tgggcggata tccccagcgc caggtgcgtt
      301 tgccgtcgcg ggttgaaagc tcgttgtccg cccatccaat gccggggtga tagtattcgg
      361 cctcgcaata gaccagttcg ccaaaaagcc cgtcctgatg aaggcggcgc gcggtgatcg
      421 tctccgggcg gtaatggctt gtttccgcca tcatataggt aagcccggtg cgctctttcg
      481 cttccttcat ggccgcggct tcctccagcg agcaacaggc gggaactgcg ctgaccacat
      541 gctttccgtg ttccatgcaa tcgatcacgt ggcgggcgtg gttggtggct tccgtgaata
      601 cgcccaccgc atcgatggcg gggtcctgga tgagctgttc gagcgactcg taagtcttct
      661 cgcagccgta tttcttctgc aagcgttcgc gccgctcggg gatgaggtcg ctgacggcct
      721 cgacgatgca attcgggtgt tcatgccagt ggaaagagag gccaaaccct ccccccacaa
      781 tcccgatgcg gatcttcttg tcggaagtct gctgccaggg cggtccagcg gccagggcct
      841 ctgcggatac ccccgccagc acgatgccgg aagcgctggt cgcggtggct ttgatgaagc
      901 cgcgccgcga gaggggcgca gtctccaggg cgtcacggtg tctcatttgg cattctcctg
      961 aaggttcatg ttctcaggcg cgggcacgcc cggctggatc gaaactgggg atcgggagct
     1021 gttcaccgtc ccggaacgac gattcgtgcg ccacaatccc gggcacggtg aacgccagcg
     1081 cttcgtaaag atcaaccagg ggctcccgct cctcgacgag cgccataata aactcgttgg
     1141 taaggaatgg atgcgatttt ccgtggccgg tgtcgtagcg catggcttcg ggcaccatgt
     1201 gccagtaatc cggcaggccg ctcagggtct ggtcgggtaa ctgcagagcg aacggctgcc
     1261 ctcccgagcc gggcatgtac atggcgccct gcataccgaa ccattgcgcc cgctcgccat
     1321 gggccttgat gtcccacgcg acattgcagc ggaacggatg tccccggtcc gtgaggaaca
     1381 tagccatgcc attggtaaag ggattgtcgt acgcattctc gcgcaaggcg gtatcatgct
     1441 cggcgtcgcc ccagccaatg caggagactt tgacaagccg ctcgcccgta acgccgacga
     1501 ggaaggcgga gctgtgagtc gggtagagca tcggtggata ggcgtaacgc caggtgcgtt
     1561 gatcattgta ataccagagc gcggcgcggc cggccttgtc catggggtgg tagtactcgc
     1621 cctccgtata gatgagctcc ccgaggaggc catcctgaac cagccgccgc gcggtgatgg
     1681 tttcccagcg atagctgctg gtttccgcca tcatgtacgt caggccggtg cgttctttca
     1741 cctctttgag gagagccgcc tcatccagtg tgcagcaggc gggtacggcg ctgatcacgt
     1801 gtttgccgtg ctccatgcag tcgactacat ggcgggcatg gttcggcgct tcggtaaaca
     1861 cggcgaccgc gtcgatggcc ggatccagaa tgagcttttc cagcgactcg taggtcttct
     1921 cacacttgta ttttccctgg agctgggtca gacggtccgg cctcagatcg ctgaccgcct
     1981 cgacaatgca gttgggatgc tcgtgccagt gaaacgactg cccgaagccc cctcccacga
     2041 cgccgatgcg tatcttcttg tccgaacgct gctcccatac cggggtatcg acaagcgatt
     2101 gaccgatcgc cgacgtcgcc agcggaaggg ccagagatgc cgaagcgctg gccagaaagc
     2161 cgcgccgtga catggaaaac gggtgggggg ttttgctgtt gagttcgtgc attgggactc
     2221 ccctgggcgc tcaccgcgcc atgcagacct ggtcaaagca aacattaaag cacgtaaacg
     2281 aacgtttcgc aacatgaccg agatcggaga ttcgcgcggc cgttgacgtt ccaggcgcgc
     2341 tggttttctc tttcagagct gcccgccgtc ctttccggcg cggcggcttt ccatgcggga
     2401 gcgaaacggg tttcggtgct cacgcgggaa cgactttcac ccatactttg cgggagcgag
     2461 gcccatcgaa ttcgcaaaaa tacagggact gccaggtgcc gaaggctagt cttccgccgc
     2521 gcacggggat tgcctggctg aaacccatca tgctggcttt gacatgggct gccgcattcc
     2581 cttccgcatg ggcatagccg gctttccagg gaacgacccg ctcaagagcc tgaatcatgt
     2641 cggcggtaac gtcggggtcg gcattttcat tgatggtgac gcccgcggtc gtatgcggca
     2701 cgaacacgaa gcagatgccg tcacgaatgc ctgttgccgc aacggcgctt tcgattttgg
     2761 cgtctatgtt cacaaattgc gtgtgactcg tggtgtgtac agcgatttct ttcatgaata
     2821 tcccctcatt ccaggtgctg gattacggcc gggctccggg tacaagcgca gcggtccggt
     2881 gctgttttca gcataccgga attccggtga aaacgcatgc cgctgggcga aatttcccgt
     2941 tggaagcgct ttttcggccc ccgttccagg caagttgaca ctacggatgc gcgctactac
     3001 cataaggaac aatttcgcgc aaaaacgcac aaccccgaac gccgatattc ggcgatctca
     3061 agaaggaaaa tgcccgtgcc cgtccgatct cccctgcgtt tcgcatctcc ccggctgagc
     3121 cttcaggatc tgacgggaaa agactatatt gaggccgtct gcagtgcgga ttcatttctc
     3181 agcgggacgt ccgcgacaga attgcgccgg atcgcggcgc agaaggtggc gttcttccct
     3241 gtctctcttc aacgggcatt gtcgtcccgc ctgccccagg tcggcacaga gtgcatccct
     3301 ccggcgggag cgacatgcac aggcgcaacc agcgtgcagt ttgcgcgaaa ttcgactcgg
     3361 gaacaggcgc cggtcagcgc gctgggatat taccggattg gcgaagacgg acggctctac
     3421 ctgacatcca agagcgagca ttaccatgcg ccgctggggc accggttccc gggctatgcc
     3481 ctgctcaatc atgcccgggc gctgggcatc ccgaatgcca cgcacaacaa tacccgaggg
     3541 cacatcaccc gccttctcga gcaggaaatg gtgcgcgccg ccgccggaat cgcgccgggt
     3601 gacgcccaga tgctccagcg gaccctgcgt tccacgtcca tgaacgtatt gaaccgcgtg
     3661 cttaacctgg agaccggcag tctggccgcg gaagcggctc tgaaaatgat cctggcgcga
     3721 tttttccgca gcgaggccgg cagtcccacg cccccctacg cggggcggat cccggtcgtg
     3781 gtggtgctgg gggatgaaga aggcggcctg gaggccaatt accacggcac cacatttctc
     3841 acccagacca tgcgcggcat gtggcctgag gtggccgccc tgttggaaca gagcggcgcg
     3901 ttcatggtac gggccgtacg accgaacaac ctggaaaatc ttgaggcggc attcgcgcaa
     3961 tgggatcagc ctccatataa gatcgccggt tttttccatg aactcattct gatgaactac
     4021 gcggcgatac ggctctcaaa agcctttctc cggcgggcct acgcgctgtg ccgccaacac
     4081 gacgtgcccg tggtctgcga tgagattcaa tcctgcgtgt ggagtccgga tctcttcctt
     4141 ttccgtgagt acgggctgaa accgagtttt gttgtggttg gaaaggggtt tccgggaggc
     4201 gaatattcgg cgtcgcgggt gctgttcagc gcggccatgg attccctgcc gcagttcggc
     4261 gcgctggtga cgaatggcca ggaggaactg gcctcgctcg cctacctgat caccatgcgc
     4321 tgggttgaag ccaaccggga acacatcgag accgtcggcg cctattatga ggaacgcttg
     4381 agggaactcg ccagccggca tgccggcgtt atcaccgcga tcgaagggcg caggcacctg
     4441 gcaggggtgt tttttgacga tgtcgagctt tcaatacaat ttgcagagca gctgagcgcc
     4501 ggtgggctgg acatcagcgt gcagacctac aagaaggggt gtcctccgag cgcgctcacc
     4561 aaactgccac tcatcgcggg gacagacgtc gtcgatgtga tcgttgcccg catggagcag
     4621 gccctgcagg ccttgatcaa gagaggcg
//
LOCUS       DAOPBQ010000283         4612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15517_length_4612_cov_3.652622, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000283 DAOPBQ010000000
VERSION     DAOPBQ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15517_length_4612_cov_3.652622"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="PKW60_09610"
     CDS             <1..1032
                     /locus_tag="PKW60_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPJ99728.1"
                     /translation="DASLVFAFCHNRDRGDLIIASRYAQGGSYAMPWRRSIISRILNR
                     LYRLVLYLPVHDLSSGRRMYRTSMLRRTPIEGDGYAVLMEVLLRCMSRGGEVRELPFH
                     YESNARRQEPGALAGQVCSSLRTLLRMHALRNSIDFPDYDYRAYDSRIWLQRYWQRKR
                     FQIVRRFTEGPGRVLDVGCGSSRIITTRPEMFGLDININRLRFLRDKLPRRLLATAGA
                     LPFADEQFDIVLSSQMIEHTAELNCVQEAARVCRRGGVVVIGTPDYSTLWWPIIERLY
                     ALLRPSAYADEHIMHYTQTSLIRELKRCGCEIEECAYVCRSELIVKAVKRRHLPTGDE
                     PCALRLNRD"
     gene            1328..2332
                     /locus_tag="PKW60_09615"
     CDS             1328..2332
                     /locus_tag="PKW60_09615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPJ99729.1"
                     /translation="MKKWTVMMIPHGKGATRNLSLSSFHLWVTVASFCVLAFASTFTL
                     QLYRNSLAQVDALERQTFHLRTATRHEPSRPAEGISTEELAAIESKYREDYKKSIDSI
                     TSKLNRLYELESQIREVHDLPAKVRSSSDYIRDGVGGVGGPPADLSEMVAPGDDELTR
                     PSNWIYGLSQPSADLIIQEINLRVASLEELLTATAEKRERIACTPNILPTLDARRRIS
                     SRFGNRKDPFSKRLQFHAGLDISATPRSPVLATAKGSVARSERDGEYGNVVEIDHGNG
                     FRTMYAHLDKLLVKAGDTVMRGDTIGLLGTTGRSTGYHVHYEVFKNGKNIDPESTVVN
                     "
     gene            2335..2757
                     /locus_tag="PKW60_09620"
     CDS             2335..2757
                     /locus_tag="PKW60_09620"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPJ99730.1"
                     /translation="MLDSGRKKTYNETNVVTIIGQGTTIIGELKSKGTVRIEGVISGR
                     VQCEDTIVVQETGRVKAEMIAGQIIIGGEVEGNVFAHDRLEVTSKGKLLGDITAPRIA
                     IAEGVLFEGKCQMKPPGQIKPPAGEAPAAPGQAPAAQG"
     gene            2825..3892
                     /gene="mnmA"
                     /locus_tag="PKW60_09625"
     CDS             2825..3892
                     /gene="mnmA"
                     /locus_tag="PKW60_09625"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPJ99731.1"
                     /translation="MATERARVVVAMSGGVDSTVAAGLLQQAGHDVMGVTMRLSSFHS
                     GGPDPREQTIEDARQAAARLGIPHHVAELTGAFQRQVLDYFLTEYQHARTPNPCVRCN
                     QQIKFGALLAEARELGGQFLATGHYVRLVSHGGRYAVARAVCREKDQSYVLSGLSQEQ
                     LGHVLFPLGELTKEETRAFARSLGFDTVAEKSESQDICFLAGGDYRSYVAQEGVPGTP
                     GPIISSSGTVLGTHTGLANYTIGQRKGLGIAGPRPYYVLRLLPERNAVVVGFHEETFA
                     ARLTTRGVVWMGFPPQREPFECQIQIRYRHEAVPCTIEPAPGHFSAVFHTPQRAVTPG
                     QWAALYRDDMVLASGAIDTFE"
     gene            3961..>4612
                     /locus_tag="PKW60_09630"
     CDS             3961..>4612
                     /locus_tag="PKW60_09630"
                     /inference="COORDINATES: protein motif:HMM:NF023980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HPJ99732.1"
                     /translation="MTARIIALAALFAISAVLSPAYGAERQITTSAKNHNLDNNDNFS
                     SDGRFLCYDTRETVGSGIGNGQTIEKVELATGVETILYQPEKTVIGEDAAPGVGAVTF
                     CPVANAVAFIHGPLLDEVPERGYYAKTNRVGAEVIADGSQRRVWLDRRDVATGRDTLP
                     GAHRGGTHRHEYSFDGKRVGFTYDDALLTEYGRTIGMLAPHPQAPEGATHYFALLVR"
BASE COUNT          891 a         1414 c         1415 g          892 t
ORIGIN      
        1 gacgcttccc tggtgtttgc tttctgccac aacagagacc ggggggatct gatcatcgcg
       61 tcgcgatacg cccagggcgg ctcctacgcg atgccgtggc ggcgcagcat tatcagcaga
      121 atcctgaacc ggctgtaccg gctggtcctg tatctccccg tgcatgacct gtcgtccggg
      181 cggcgcatgt accgcaccag tatgctccgc cgcacgccga ttgagggcga tggctacgcg
      241 gtactcatgg aagtcctgct gcgatgcatg tccaggggcg gcgaagtgag agagctgccg
      301 tttcattatg aatcgaacgc gcgccgtcag gagccgggcg ccctggcggg gcaggtgtgc
      361 agttcgttgc gcacgctgct gcgtatgcac gcgctgcgta acagcatcga ttttcccgac
      421 tacgactacc gggcttacga cagccgcatc tggctgcagc ggtactggca gcgaaagcgg
      481 ttccagattg tgcggcggtt caccgagggg cccgggcgcg tgcttgacgt cggttgcggt
      541 tccagccgca tcattaccac ccgcccggag atgttcgggc tggacatcaa catcaaccgg
      601 ctgcggtttc tgcgcgacaa gcttccgcga aggctgctgg cgaccgccgg cgctctccct
      661 tttgccgacg agcagttcga catcgtcctc agttcgcaga tgattgagca cacggccgag
      721 ctcaattgcg tgcaggaagc cgcgcgggtc tgccgccggg ggggcgtcgt tgtgatcggc
      781 acgccggatt acagcacgct ctggtggccc atcatcgaac gtctttatgc cttgcttcgc
      841 ccctccgcgt atgctgacga gcatatcatg cactataccc agacctcgct gatccgggaa
      901 ctgaaacgat gcggctgtga aattgaagaa tgcgcctatg tctgccggag cgagctcatc
      961 gtgaaggccg ttaaacggcg ccacttgccg acgggagatg agccgtgcgc cttgcgtctc
     1021 aaccgggact gagtcgtcga gattcagcga gtcaggcagg ctttcaacga gctgtgcccc
     1081 gccgggttcc gcgttctctc tttacggaat tacttattat aataaaacga tttaagtgcg
     1141 attttcgggc tgttcttgcg ttgacttcat tttggtgccg tgctatagtc cgaagaacag
     1201 ctggaactga actgcatctt cagggataaa gttggggagg cgcgtgggag ctcaattggg
     1261 gagatttcgc accctcatct gttccaggta aattgtgttt ttatctactc gcgggagtcc
     1321 ggtttatatg aagaagtgga ctgtgatgat gatcccccat ggcaaagggg cgactcgcaa
     1381 tctaagcctt tcttcctttc atctctgggt cacggtcgca agtttctgtg ttctggcctt
     1441 cgcctccacc tttacactcc aattataccg caattcgctc gcgcaagtgg acgctttgga
     1501 gcggcagacc tttcatttgc ggacggccac gcgccacgag ccgtcgcgac ctgccgaggg
     1561 tatttctacg gaggagctgg cggccattga aagcaaatac cgtgaagatt acaagaagag
     1621 cattgattcc attacaagta aattgaatcg gctgtacgag ctggagtcac agatacgcga
     1681 agtgcatgac ctgccggcca aggtgcggtc ttcctcggac tatatccgcg atggtgtggg
     1741 cggcgtgggc ggccctccgg ccgacttgag cgaaatggtg gcgccgggcg atgacgagct
     1801 cacgcgcccg tcgaactgga tttacggttt gtcgcaacct tccgcggatc tcattatcca
     1861 ggagatcaac ctgcgcgtcg ccagcctcga agaactgctg acggccacgg ctgagaaacg
     1921 cgagcgtatt gcctgcacac cgaacatcct gccgacgctt gatgcgcggc ggcggatcag
     1981 ctcccggttc ggcaaccgta aagacccctt ctcgaaacgc ctgcagtttc acgctggcct
     2041 cgacatctcg gccacgccga ggtcgcccgt gctggctacg gccaaaggca gcgtggcccg
     2101 gtctgagcgc gacggcgaat acggtaatgt tgtcgaaatt gaccatggga acgggttccg
     2161 gacgatgtat gcgcacctgg acaagctcct ggtgaaggcc ggcgacacgg tgatgcgcgg
     2221 cgacacgatt gggttgctgg gcacgaccgg ccggagcacc ggctaccacg ttcattacga
     2281 agtcttcaaa aatggcaaga acattgaccc tgaaagcact gtggttaact gaacatgctt
     2341 gattccggaa gaaaaaagac ctacaacgag acgaatgttg tcaccattat cggccagggc
     2401 acaaccatca tcggcgaatt gaagtcgaaa ggcacggtgc gcatcgaagg cgtcatctcc
     2461 ggccgcgtcc agtgtgagga cacgattgtc gtgcaggaga cgggccgggt caaggccgaa
     2521 atgatcgccg ggcagattat catcggcgga gaggtcgagg gcaatgtatt cgcccacgac
     2581 cgcctggaag tcacctccaa gggtaaactg ctgggcgata tcaccgcgcc gcgcattgcc
     2641 attgccgaag gcgtgctctt cgaaggcaag tgccagatga aaccgccggg ccagatcaag
     2701 ccgcctgcag gcgaggcgcc ggccgccccc ggacaggcgc ccgctgcgca aggataagcc
     2761 gcgccgcgcg tgccggcaat acgatgccgg cgccggcgtt ccccacgaga gacgatctcc
     2821 cacgatggct acggagcgcg ccagagttgt cgtcgcgatg agcgggggcg tggacagtac
     2881 tgtcgcggcc ggcctgttgc aacaggccgg acacgatgtg atgggcgtga ccatgcgcct
     2941 gtcctctttt cacagcggtg gcccggatcc ccgggaacag accatcgaag acgcccgcca
     3001 ggcggctgcc cgcctgggca ttccgcatca tgtcgcggaa ctcaccgggg cttttcaacg
     3061 ccaggtcctg gactattttc tgactgaata ccagcatgcc cgcactccca acccctgcgt
     3121 ccggtgcaac cagcagatca agttcggggc gctcctcgca gaggcccgcg agctgggagg
     3181 gcaattcctc gcgacgggcc attatgtccg cctggtctcg cacggaggcc gctatgccgt
     3241 ggcccgggcc gtctgccgcg aaaaagacca gagttacgtg ctgtccgggc tcagtcagga
     3301 gcaattgggg catgttctct ttccgctggg agagctcacc aaagaggaga cacgggcgtt
     3361 cgcccgctca ctcgggttcg acacggtcgc ggagaaatcg gagagtcagg acatctgttt
     3421 cctggcgggt ggcgattacc ggagctatgt cgcgcaggag ggcgtgcccg gcacgccagg
     3481 acccatcatc tcgagttcgg gcacggtgct cggcacgcat accggtctgg ccaactacac
     3541 gatcgggcag cgaaaaggcc tcggcatcgc gggcccacgg ccctattacg tgctgcggct
     3601 ccttcctgag cgcaacgcgg tggtggtggg cttccacgag gagacttttg ctgcacgcct
     3661 tacaacccgc ggtgtggtgt ggatgggttt tcccccgcaa cgcgaaccgt ttgaatgcca
     3721 gatccagatc cgctaccgcc acgaagctgt tccctgcacg atcgagcccg ctcccggcca
     3781 tttttccgcc gtgttccata cgccccagcg tgccgtgact ccgggacaat gggcggccct
     3841 gtaccgcgac gatatggtgc tcgcatccgg tgctatcgac accttcgagt gaactgcgtt
     3901 gcatccggtt tgcggggctg ctagactgcc gcgaaccaca acccaaccgc gaggatcgcc
     3961 atgactgcaa gaatcatcgc cctggctgcc ctttttgcca tcagcgcagt gctctcgccg
     4021 gcatatggcg cggagcggca aattaccacc tccgccaaga accacaacct ggacaacaac
     4081 gataatttct catccgatgg ccgtttttta tgctatgaca cccgggaaac cgtgggttca
     4141 ggtattggca acggccagac tatcgagaag gttgagctcg caacaggcgt ggagacgatc
     4201 ctctaccagc cggaaaagac cgttatcggc gaggacgcgg cacccggggt gggcgcggtc
     4261 acgttctgcc cggtcgccaa tgcggttgcg tttattcacg gccccctcct cgacgaagtg
     4321 cccgaacgcg ggtattacgc gaaaaccaac cgcgtggggg cggaagtcat tgccgacggc
     4381 agccagcggc gcgtctggct ggatcggcgc gatgtcgcca ccggccgcga caccctcccc
     4441 ggcgcgcacc gcggcggcac ccaccggcac gagtattcct ttgacggcaa gcgcgtcgga
     4501 ttcacctacg acgacgccct gctgaccgaa tacggccgca ccatcggcat gctggcgccg
     4561 catccccagg cgcccgaagg cgcgacgcac tacttcgccc tgctcgtccg cg
//
LOCUS       DAOPBQ010000284         4601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15565_length_4601_cov_3.335240, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000284 DAOPBQ010000000
VERSION     DAOPBQ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15565_length_4601_cov_3.335240"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PKW60_09635"
     CDS             complement(<1..651)
                     /locus_tag="PKW60_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPJ99733.1"
                     /translation="MDTDSNRRSDPLHFAALMAHQLRSPLDAALSQMGLLLEGYAGKL
                     TAQQRDLLERIQRRGREAMDAANRVLAIARAMESVEPPSSVADLTQVVRGAERRYTSE
                     AHRRKMHMHCDTPGEPVWVHASEDELSELCLALLNNAFKYTPDHGRVRAGVSVSPDGK
                     RVRLVVEDSGIGIPETERETVFQPFTRTLNASGSTAPGVGLGLAFVHAMVRRAGGHV"
     gene            complement(901..3525)
                     /locus_tag="PKW60_09640"
     CDS             complement(901..3525)
                     /locus_tag="PKW60_09640"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPJ99734.1"
                     /translation="MRAELAAALLCLLTAAAGAELMAEVKDYLGSPTLFVNGVPRTPM
                     VFYGWDQGPGPSVTHLGTDWREHHVTFTAPEDDAGDCGVHLRFGNAPGTVWIDDVRFY
                     EGEYRPDVTENRLRCGDWDCDRSAADDAWTLFVRPEAEASWALDTSVKHSGTQSCRLT
                     IAKAGTNPMHIHFHQTGLSIEKGKTYTFSAWLRADAERTGDMMVLHHGPPWTIYSGPE
                     DSWLHKQVTMAAAEGVHIHSFGIDMPWPKPGERPDFSETDAVLEHVLEADPEALMLPR
                     FACEPPGWWYETHPDEALQYHDGSRRPVCVASTAWREELQRNLRALVEHCEARYGENM
                     LGYHPCAQHTGEWFYPGTWDSLHSGFSPAMQRGFSAWLRTKYATDEALRTAWGDSDVT
                     FDTVTVPSVDERETASVGLFRDPAREQKTIDFYEYLQVAMVEPLELIAATVKEASARK
                     KLVTLFYGYYFEISGLPFGPQTSGHLALARLLECPDVDIVCSPISYEDRGLGGIGAFM
                     VPVDSVRDHGKLWLNEDDTRTFLTPEDAGYGRVSTLEHTRWVHQRNFGRMLPRRLACW
                     YMDLGGAGWLADERLWRNIGELRAIYDAQLEKPATWTADVAVIVDETSPFYLASNADV
                     MRPLANHFRRQLYRMGVPFRQYLLSDLEAGRVPEHKAYLFIGCFHLNASQRGVIADAV
                     AGKTALWFYGSGFVDDRPDSANLAALIGMPVREVEGDAVAMITPDPEAPLCADVPREP
                     FGTPAALRPLWGIEEEGGIEVLGRFSDGTAAAAALRRNEGMTVYIGTVNAPAKLLRNI
                     LKASGVHVYLDSDDALAADGAFLSVTATSAGIKAIRLPRPSEVQRVLPEPGELGRTAE
                     FAEPFEEGETRIYLLRND"
     gene            complement(3630..4427)
                     /locus_tag="PKW60_09645"
     CDS             complement(3630..4427)
                     /locus_tag="PKW60_09645"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPJ99735.1"
                     /translation="MTLRDAFLEGGGCPVIDAHAHMGPFRSIYLPEASRERMIAGMER
                     AGIEAIIVSPHDALFGDTREGNAQMLDAVQACPGRIYGYCTIHPGYPRETESELDRYL
                     HQPGVVGVKIHPTSHEYPVDGLNYAPVLERLEAEGGLLLSHTWGNDNFCGAAKMRLVA
                     ERYPNIRLLLGHSCYGAWTEAIALAAEFPNVYLELTAAAHVYGLIEWMCREAGSRKVV
                     FGTDYPWFDPAVYIGFVTFAHIDEEAMRNILYTNARTLLDEQLAKRK"
BASE COUNT          859 a         1568 c         1334 g          840 t
ORIGIN      
        1 gacgtgcccg ccggcccgcc tcaccatcgc atgaacgaag gcaagcccca gaccgacacc
       61 cggcgccgtc gagcccgaag cgttcagcgt gcgcgtgaac ggctggaaca ccgtctcgcg
      121 ctccgtctcg gggattccga tcccggagtc ttcgaccaca agccgcaccc gtttgccgtc
      181 cgggctgacc gaaacaccgg cacgcacacg gccgtggtcg ggcgtgtact tgaacgcgtt
      241 gttcagcaac gcgaggcaca gttccgataa ttcgtcctcg ctggcatgga cccagaccgg
      301 ctcgccgggc gtgtcgcagt gcatatgcat cttccggcga tgcgcctcgc tcgtgtaccg
      361 ccgttccgcg ccccgcacca cctgggtcag atcggccacc gacgacggcg gttctaccga
      421 ttccatggcg cgcgcgatcg ccagcacgcg attcgccgca tccatagcct cacgtccgcg
      481 ccgctggatc cgctcgagca ggtcccgctg ttgggccgtc agcttcccgg cgtacccttc
      541 aagcagcagg cccatctgcg acagcgcggc gtccagcggc gaccgcaact ggtgcgccat
      601 caaggccgcg aaatgaagcg ggtctgaccg tctgttgctg tccgtgtcca tcgaagctac
      661 ctttctggcg cttcgagtgt accggttctt gcccggggag taaacgggcg gctggagccg
      721 cgagcgcaat tagggtgcca acagcacccg gggcacgcag cctggaggat ggaagcccca
      781 gcctgtccgc gccgcacgta aaaacaggcg ggagcgccgg gaaatgactc cacagacgga
      841 tcctgccaca gttcgtttcg gttttgttgc gcagacgttg cgtgcaacgt tacagcgcgt
      901 tcaatcgtta cgcaacaagt aaatgcgcgt ttcaccctcc tcgaagggct ccgcaaactc
      961 ggcggtccgc cccagctcgc ccggttcggg caggacgcgc tgcacctcgg agggccgcgg
     1021 caggcggatc gctttaatcc cggcggacgt cgccgtcacg ctcaaaaatg cgccgtcggc
     1081 cgcgagcgcg tcgtcggaat ccagatagac atgcacaccc gacgccttga ggatgttgcg
     1141 caggagcttt gcgggagcgt ttaccgttcc gatgtagacc gtcatgcctt cgtttcgccg
     1201 cagggcggcc gccgctgcgg ttccatcact gaagcggccc aatacctcga tcccgccctc
     1261 ttcctcgatt ccccacaggg gccgaagcgc agcaggagtg ccgaaaggtt cccgcggcac
     1321 atccgcgcac aggggcgctt cgggatccgg cgtgatcatg gctacggcgt ctccctcgac
     1381 ttcacgcacg ggcatcccaa tcagggcagc caggttcgcc gagtccggcc gatcgtcgac
     1441 aaatccgctg ccgtagaacc acagcgccgt cttgcctgca accgcatccg cgatgacgcc
     1501 tcgctgcgac gcattcaggt gaaaacagcc gatgaacaga tacgccttat gctctggcac
     1561 gcggcccgcc tccaggtcgc tcagcagata ttgccgaaac gggaccccca tccggtagag
     1621 ctgccgccga aaatggttcg cgagagggcg catcacgtcc gcgttactgg cgagatagaa
     1681 cggactcgtc tcatccacga tcaccgccac atccgccgtc cacgtggcgg gtttttcgag
     1741 ttgggcgtcg tagatggcgc gcagttcacc gatatttcgc cagaggcgtt cgtcggccag
     1801 ccagcccgcg ccgccaaggt ccatatacca gcacgccagg cgccgcggca gcatgcggcc
     1861 gaagttgcgt tgatgcaccc agcgcgtgtg ctccagcgtc gacacccgcc catagcccgc
     1921 atcttcgggc gtcagaaagg tgcgcgtatc gtcttcgttc aaccagagtt tgccgtgatc
     1981 acgcaccgag tctaccggca ccataaacgc gccgatcccg ccgagaccgc gatcttcgta
     2041 cgagatcggc gaacagacga tgtccacgtc cgggcattcg agcagccgcg ccagcgccag
     2101 atggccgctc gtctgcgggc cgaaaggcag gccgctgatc tcgaagtagt agccataaaa
     2161 caacgtcacg agtttttttc gcgcacttgc ttctttgacg gtcgcggcga tcaattccag
     2221 cggctccacc atggccacct gcaggtactc ataaaaatcg atggtcttct gctcccgtgc
     2281 ggggtcgcga aacaggccca ccgatgctgt ctcgcgctca tcaaccgacg gcacggtgac
     2341 ggtatcgaaa gttacgtccg aatcgcccca cgccgtgcgc agcgcctcat ccgtggcata
     2401 ttttgtccgc aaccacgccg aaaatccgcg ctgcatcgcc gggctgaagc cgctgtgcag
     2461 gctgtcccag gttccgggat aaaaccattc gcccgtgtgc tgcgcgcagg gatgatagcc
     2521 gagcatgttc tccccgtagc gcgcttcgca atgttcgacc agcgcccgca ggttccgctg
     2581 cagttcttca cgccacgccg tcgacgctac gcagaccggc cggcggctgc cgtcgtgata
     2641 ctggagggcc tcatccggat gggtctcata ccaccatccc gggggctcgc aggcgaatcg
     2701 cggcagcatg agcgcttcgg gatcggcctc cagaacatgc tccagcaccg catccgtctc
     2761 tgaaaagtct ggccgttccc ccggtttcgg ccagggcata tcgatgccga aggagtggat
     2821 atgcacgcct tcagccgccg ccatcgtcac ttgcttgtgc agccacgaat cctccggacc
     2881 cgaatagatg gtccacggcg gaccgtgatg cagcaccatc atgtcgccgg tccgctccgc
     2941 atccgcgcgc agccacgcgg aaaaggtata ggtcttacct ttttcgatcg acaagcccgt
     3001 ctggtggaaa tggatgtgca tggggttggt accggctttt gcgatcgtca gccggcagct
     3061 ctgtgtgccg ctgtgcttca cggaggtgtc gagtgcccac gacgcctccg cttccggccg
     3121 cacaaacagc gtccaggcat cgtccgccgc gctccgatcg cagtcccaat cgccgcagcg
     3181 caggcggttt tccgtgacat ctggccggta ttcgccttca tagaaccgca catcgtcaat
     3241 ccacacggtc cccggtgcat ttccgaagcg caggtgcacg ccgcagtcgc ccgcgtcatc
     3301 ttccggcgcc gtgaacgtca catggtgttc gcgccagtct gtgcccagat gcgtgacgga
     3361 tgggcccgga ccctggtccc acccgtaaaa caccatcggg gtccgcggca caccattaac
     3421 gaacagcgtc ggcgacccga gatagtcttt gacctccgcc atcaattctg ctccggccgc
     3481 agccgtcaaa agacacagca gggctgccgc cagttccgct cgcataataa gtcgccactt
     3541 caccgttgga attccctttc cagaaaaaat gaggctgtcc cgcaggcttg ggcgggtaac
     3601 ttacaggtga cccggccatc acggacgcac tacttccttt tcgccaattg ttcatccagc
     3661 agggttcgcg cattggtata gaggatattt cgcatggcct cttcatcgat gtgcgcaaac
     3721 gtcacgaatc cgatgtatac cgccgggtca aaccacggat agtctgtccc gaaaaccact
     3781 tttcgtgagc ccgcctcccg gcacatccat tcgatcagcc cgtacacatg cgccgccgcg
     3841 gtcagttcaa gatagacgtt tggaaactcc gctgccagtg cgatggcctc tgtccaggcg
     3901 ccgtaacacg agtggccgag gagcagccgg atgtttggat aacgctcggc gacgagccgc
     3961 atctttgctg cgccgcaaaa attgtcattg ccccaggtat gcgacagcag cagcccgccc
     4021 tctgcctcca ggcgctccag caccggcgca taattcagcc catcgaccgg atactcatgc
     4081 gaagtcgggt ggatcttcac gcctaccacg cccggctgat gcagatagcg gtcgagttcg
     4141 ctttccgtct cgcgagggta tcccgggtgg atcgtgcaat aaccatagat ccgtcccgga
     4201 caggcctgaa ccgcgtccag catttgcgcg ttgccttccc gggtatcgcc gaaaagcgcg
     4261 tcgtgcgggg agacgatgat cgcctcgatc ccggcacgct ccatgcctgc gatcatgcgt
     4321 tcacgggagg cttccggcag ataaatcgac cgaaacggcc ccatatgcgc gtgggcatcg
     4381 ataaccgggc agccgccgcc ctcgaggaat gcgtcacgca gcgtcacaac gacacctccc
     4441 ccaggagacc ggccagattg ccgtgtgcga tggcctgccg ggcctcgtcg gagatgtccg
     4501 cccgcgcgag catcatgatg tagccgccgg gattattgaa cgacagcccc gacccgaaca
     4561 gcagccgctc gtatcccaga tcgttcacga acgatttcag c
//
LOCUS       DAOPBQ010000285         4590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15608_length_4590_cov_3.574642, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000285 DAOPBQ010000000
VERSION     DAOPBQ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15608_length_4590_cov_3.574642"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PKW60_09650"
     CDS             <1..334
                     /locus_tag="PKW60_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ99736.1"
                     /translation="AEQYLLSPAAGMTGMRMPLVALGDVSARLGDPKRAYEYLTRATD
                     AYPGEPAPWLALCDLSIAQHNALAARQYLAEAEKCNAPREEIAQREDKLGAGSPAVPA
                     QPGEVIIR"
     gene            complement(366..2726)
                     /locus_tag="PKW60_09655"
     CDS             complement(366..2726)
                     /locus_tag="PKW60_09655"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPJ99737.1"
                     /translation="MSVTAKWTGLILLLVVIGCGCQTSPVYRPGQEFRTINAAEYEIA
                     VQKSGRVNVLLRSGQVILANAQPMVWYEQDSSPKPLPVLGRLTVREQVNDRLGQGQGM
                     LFKQKECEWAIRVYPDKPFFTCQAAYVNRSKKPVKIRALIPWFVGDATSGQVSLGSEP
                     ESTVILQNSTPFAGIPQMPSITRGEGESLWYLAGFNPVSRQSLIAGFVTSDTGYGSIT
                     LGRSKKAKTPALNQFSATCYFDPPVEVMPGGKVQSEVLYISVAEKTPLEGLERYGHAV
                     AAVNEVPVRKPPLPHGTTVLSKTHGQLSEEAVRQEMDVLEADLLRYGWNNVAIGGGWE
                     AAPGVWEPDPVAFPRGFAPLVQEIHARGMTASLYIDPFVVPADSPLAASHPGWFAPVA
                     SWAAPLLPENARILDITVPEADAYIRDLFQKVGTQWGFDSIEGVQITRGILAAESFSD
                     TSLTRADVLRLAISAVDQGLGQGKGFAAMGAQPALHVRADAVSVLDGVMPLWQGDAEH
                     PGFQEALQAAAFRYFFSPYLYAPYMGPAYFNTPGLAERWPELRLDSLTTAQSTAWLTA
                     MAINGSVMKIGNRFTELNADQKKILSRLLPVLPWTARPLDLFESSLPAVWSAQLRTKA
                     GDWHYAAVFNWSTSEQHTATLYLGELGMNPQSVYTVYDFWNDQFHGLAQGQLNVQAPP
                     GGVRLLGFRLYENHPLLVASTTHFAQGALDTASLEWDAAARELRGETRAIPNTDYGLR
                     ILVPDSFSVSEAAVSGVQPSLKKDGQLLEMNFHNGTAQSFRWYVRF"
     gene            complement(2770..3795)
                     /locus_tag="PKW60_09660"
     CDS             complement(2770..3795)
                     /locus_tag="PKW60_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99738.1"
                     /translation="MAKDKILAAATLGAGSIAQHCHMPGYAKSPHTELVAFADPAPAR
                     HREIKKLYPSARGYKSHEEMFANERLDVVSICTPNKYHTEGALAALAAGCHVLCEKPM
                     ATRVSDAEKMLAAAKKARRKLMIGFTHRTMAGSKKCKELLARRALGKPFMIRLRYAHG
                     GPYPGWAKGDWFYSREVAAGGAMLDMGIHAIDLALWLMGPVTAVTARTATLIKPIEVD
                     DNALLLLEFASGALGYIEVGWTSKPGFTGFELYGTKGSIICDYDKGLQLCGGKASPGE
                     DSVLEWQMLEPQPAAGGWPAEMVEWIDVVRGKQRLTMTGQDGINALAVALAAYSSSKS
                     GRRVALR"
     gene            complement(4068..>4590)
                     /locus_tag="PKW60_09665"
     CDS             complement(4068..>4590)
                     /locus_tag="PKW60_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPJ99739.1"
                     /translation="LREGQDMNDIPGMPQARARRAPPSSAAREGQYLVFRLGPVDCGI
                     EIQQVRDIVGMLPAAPRPGVPSFVKGYITLREKVMPLIDLRARFALPAQEETENSCII
                     VVEAQGKEPISTLGLMADEVREVLQIPKEQMLPPVAWGAPIPTEFIYAMAHVGNTPLV
                     LLDIVRVLEPACT"
BASE COUNT          870 a         1409 c         1420 g          891 t
ORIGIN      
        1 tgcagagcaa tatcttcttt cacctgccgc cggcatgacg gggatgcgga tgcctctggt
       61 ggctctggga gacgtatcgg ccaggttagg agatccaaag cgcgcctacg agtacctcac
      121 ccgggcgacc gacgcatacc ctggcgagcc ggccccctgg ctcgctctct gtgacctttc
      181 catcgctcaa cataacgcgc tcgccgcccg gcagtatctc gcagaagcgg aaaaatgcaa
      241 tgccccgcgg gaggagattg ctcagcgcga agacaagctc ggcgcgggca gccctgcggt
      301 tccggcgcaa cccggcgaag tgatcatccg ttagcccgcc gccccgccgt gcattgctcc
      361 gcgttttaga atctgacgta ccagcgaaag ctctgcgcgg tgccgttatg gaagttcatc
      421 tcgaggagtt gcccgtcttt tttcaggctg ggctggaccc cagataccgc ggcttcagaa
      481 acactgaagg aatccggcac caggatgcgc agaccgtaat cggtgtttgg aatggcgcgg
      541 gtttcgccgc ggagttcgcg cgcggcggcg tcccactcca gcgaggcggt gtcaagagcg
      601 ccttgcgcga aatgggtggt agaggcaacg aggagcgggt ggttttcgta caaccggaaa
      661 cccagcagac ggacgccgcc aggcggcgcc tgcacgttga gctgtccctg ggccagtccg
      721 tggaactggt cgttccagaa gtcgtacacc gtatacacgc tctgcgggtt catccccaat
      781 tcgcccagat agagcgtggc ggtgtgctgt tccgatgtgg accagttgaa caccgcggca
      841 tagtgccagt ctccggcttt ggttcgcagc tgcgcggacc agacggccgg aagactgctt
      901 tcaaacagat caagagggcg ggcggtccag ggcaagaccg gcaagagcct gctgagtatc
      961 ttcttctgat cggcattgag ctcagtgaag cggttgccga ttttcataac gctgccgttg
     1021 atcgccatcg ccgtgagcca ggcagtgctc tgcgcagtgg tgagggaatc cagcctgagc
     1081 tcaggccagc gttcggccag gccgggtgta ttgaagtacg cggggcccat atacggtgcg
     1141 tacaggtagg gtgaaaagaa atagcggaag gcagcagcct gcagggcctc ctggaagcca
     1201 ggatgttccg catctccctg ccagagcggc atgacgccat ccagcacgct cacggcgtcg
     1261 gcgcgcacgt gtaatgcggg ctgggcgccc atggccgcga agcctttacc ttgccccaaa
     1321 ccctggtcaa cggcggatat ggccaggcgc aacacatccg ctcgggtcag agacgtgtcc
     1381 gagaaggatt cggcagcgag aatgccgcgt gtaatttgaa cgccttcgat cgagtcaaag
     1441 ccccactgag tgccgacctt ctgaaagagg tcgcggatgt aggcatcggc ctccggcact
     1501 gtgatgtcga gtatgcgggc attttccggc agaagaggcg ccgcccagct cgcgaccggc
     1561 gcaaaccagc cgggatggct ggcggccagc ggggaatctg ccggcaccac aaacggatcg
     1621 atgtacaggc ttgcggtcat tccgcgcgcg tgaatttcct gcaccagggg cgcaaatccg
     1681 cgtgggaacg ccaccggatc cggctcccag acgcccggag cggcctccca gccgccgcca
     1741 atggcgacat tgttccagcc gtatctcaaa aggtcggctt caagcacgtc catctcctgg
     1801 cgcaccgctt cttcggacag ctgcccgtgc gtctttgaga ggacggtggt cccgtggggc
     1861 aggggaggct tgcggaccgg gacttcgttg acggcggcga ctgcgtgccc ataacgttcg
     1921 aggccttcca ggggagtctt ttccgccaca gagatataca ggacttccga ctgcactttt
     1981 ccaccgggca tcacttcgac gggcggatcg aagtagcatg tggcgctgaa ctgattcaat
     2041 gccggtgtct tggctttttt cgatcttccc agcgtgattg agccataacc ggtgtccgat
     2101 gtaacaaacc ctgcgataag agactgccgg gagacaggat tgaagccagc caggtaccag
     2161 aggctttcgc cctcgccccg tgtgatggaa ggcatctgcg gaatcccggc aaatggggtg
     2221 gagttctgca gaatgaccgt cgattccggt tcgctgccca gagacacctg gccgctggtc
     2281 gcgtctccaa cgaaccaggg gatgagcgcg cggatcttga ccggtttttt gctgcggttc
     2341 acgtacgcgg cctggcaggt aaagaacggc ttgtcagggt acacccggat ggcccactcg
     2401 cactcctttt gcttaaacag catgccctgg ccctggccca ggcgatcgtt gacctgctca
     2461 cggacagtca gacgaccgag tacggggagc ggcttcggac tgctgtcctg ctcgtaccat
     2521 accatgggct gggcattggc caaaatgacc tggcccgagc ggagcagcac attcacgcgg
     2581 ccgcttttct ggacggcgat ctcatattcc gcggcgttga ttgtgcggaa ctcctggccc
     2641 ggccggtata cggggctggt ctggcagccg cagccgatca caacgagtaa caaaatcagg
     2701 ccggtccatt ttgccgtgac gctcatgcag gtctccgtag ttgttgtaga ccgggcgcgg
     2761 aaccgcctct cagcgcaagg ccacgcgccg cccgctcttg ctcgacgaat acgcggccaa
     2821 agccaccgcc agcgcgttga ttccgtcctg gcccgtcatc gtaaggcgct gcttgccgcg
     2881 cacgacgtca atccattcaa ccatctcggc cggccagccg cccgcggcag gctgaggctc
     2941 cagcatctgc cattccagga cactgtcttc gccaggcgac gctttgcccc cgcataactg
     3001 gaggcccttg tcgtagtcac agatgatgga acctttcgtg ccgtacagtt cgaatcccgt
     3061 gaaaccgggc ttgctggtcc atccgacttc gatgtagccc agcgcaccgc tggcgaattc
     3121 gagtagaagc agggcattgt catccacttc gatcggcttg atgagggtcg cggtccgggc
     3181 cgtgaccgcc gtgaccgggc ccatcagcca gagagcgagg tcgatcgcgt gaatgcccat
     3241 atccagcatc gcgccgccgg cggccacttc ccgactgtag aaccagtcgc ctttggccca
     3301 gccggggtac ggtccaccat gcgcgtagcg gagccggatc atgaacggtt ttcccagcgc
     3361 gcgcctggcg agaagctcct tgcatttttt actgcccgcc atcgtccggt gtgtgaatcc
     3421 aatcatcagc ttcctgcggg cctttttcgc ggccgcgagc atcttttccg cgtcgctgac
     3481 gcgggtggcc atgggctttt cgcacagcac atgacagccc gcggccaggg cggccagggc
     3541 gccttcggtg tgatatttgt tgggcgtgca aatgctcacc acatcgaggc gctcgttagc
     3601 aaacatctct tcatgggact tatatccccg ggctgacgga taaagtttct tgatttctct
     3661 gtgccgggcc ggagccggat cggcaaaggc caccagctcg gtatgcgggc ttttggcgta
     3721 gcccggcata tggcaatgct gtgcaatgga gcctgcaccc agggttgccg cggcgagaat
     3781 cttgtctttt gccatggaaa gtccactcct tcttccaggg aagtcacccg gttgtaagtc
     3841 tactcggaga aatgaccgcc ttcaaatgtc atcggccgcg aaaacctcgc ccagaagtct
     3901 ccctgccatc cggagattcg ttaaaggaac aagaacaggg tggctgccgg gcacagaaat
     3961 cagcgccttc caaagcgctc gcgtggagat gccaaacgcc tttcccgagc cgccggctcc
     4021 atttttccac ggcacccttg agactcgtgc acagttcccg ttcagggtca ggtgcaggcg
     4081 ggttcgagca cgcgcacgat atccagcagg accagcgggg tgttgccaac atgcgccatg
     4141 gcgtaaatga actcagttgg aatgggagcg ccccaggcga cgggtggcag catttgctcc
     4201 ttcggaatct gtagaacctc acgaacttca tcggccatga ggcccagggt ggaaatgggt
     4261 tctttgcctt gggcttcaac cacaatgatg cacgaattct ccgtttcttc ctgggccggg
     4321 agggcgaaac gggcgcgcag gtcgattagc ggcatgacct tctcgcgaag tgtgatgtac
     4381 cccttaacga aactggggac tcccggccgg ggcgcggcag gaagcatgcc gacgatatcg
     4441 cggacttgct ggatttcaat accacagtcg accgggccca acctgaacac gaggtactgc
     4501 ccctcccggg cagcagagga gggcggcgcc cggcgggccc gggcttgcgg catccctggg
     4561 atgtcgttca tatcctgccc ctcccggagc
//
LOCUS       DAOPBQ010000286         4582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15635_length_4582_cov_4.172520, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000286 DAOPBQ010000000
VERSION     DAOPBQ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15635_length_4582_cov_4.172520"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="PKW60_09670"
     CDS             <1..1572
                     /locus_tag="PKW60_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99740.1"
                     /translation="IEADGEAVESMHPFCRIDRPVPILAPPVGAAIDAPEPYAMLAMR
                     DLPLYTVCFAADNPELDTLVDTAIQSGLRPVIVVDLAVTPLDALSGLLERYGSRAAHW
                     TVLSGGAPGVQEPAAALFAGHEPAVPVSWAAGTPEELTALLQSSAGQPVSAVVFQHKA
                     PSAEELAAFELAAQAAGYEHLPIHILGRGIAEDGPDAAARLACNLIDFAARGVAEVNI
                     DGALLFHDQEVTEAYVFVSALVRSLAGAVPIGMLDAPAPYRAVVFRLGDSWLIAAWSE
                     GKAGSLTIPVGEAVLHELADASNNPLELPAVTEEAVSLELAAAPVYLRGEGGTILAST
                     ARRMMQNAAADFAANPAYAALLPEQVADIFAIMRKAVPPRIERSQFLALLPMFPLLEA
                     QWHSGKVPREVAVPAMSALSDIIRHAAVIEQAFDEPFVQPLQDTLATCTEFQQDFLTR
                     AEAPPETRARADWLLAEVTRLAEEAKHLNETDRTIEANAIASLAKWRAHALNATLNYV
                     APAEEAAVESSSGTS"
     gene            1587..2558
                     /gene="pfkA"
                     /locus_tag="PKW60_09675"
     CDS             1587..2558
                     /gene="pfkA"
                     /locus_tag="PKW60_09675"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPJ99741.1"
                     /translation="MKRIGVLTSGGDAPGMNAAIRAVVRAGAYYDLQVFGIQRGYQGL
                     IDGHVFEMGPRSVSGIINRGGTILRTARCQAFFEKDGRTQAADTLRKHHIDGLVVIGG
                     DGSYRGALALYEEHGIACIGLPGTIDNDIGGTDYSIGYDTALNVALEAIDRIRDTAES
                     HERIFFVEVMGRNSGYLAMMSGIAGGAEEILVPETPTSIETLVEHIHAYRAKGKQSAI
                     IVVAEGDELGDAIQVAKQVDALAKIPNSRVVVIGHLQRGGTPTAFDRILASRLGVRAV
                     EALREGVTGKMVGISTDGVVLRPLQEAWECRTQFDPKLARIAHILAS"
     gene            complement(2597..3763)
                     /locus_tag="PKW60_09680"
     CDS             complement(2597..3763)
                     /locus_tag="PKW60_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99742.1"
                     /translation="MINLIEFGPGNYRLLKRFVQFHWDHYKDHPYYVPPLNMDLLGNR
                     LLGEKGLLTDKHPFHKHAEVVYFLAERDGKVVGRIAGCINQRYNEQQNARAVIFGFWE
                     VINEFEVARALLDRVAAWGKARGMTTLLGPMSFSTNEIVGIQLNAFDTLPYYETASNY
                     PYYADLLDRYGMYKAMDVIAQKLDFTNGAADPRLMERINKIAERMARRNGLWTRPLDF
                     SNLAEERKLIRSIYNDAWKDNWGATAFTEEEFNLTADKLRTIVKSGIALLGYVHDEAA
                     AFFLILPDIYDCIKTNGSAFGRLDFVRVARLFRNKHRTRRGRVTLLGIKEPYRRSGLD
                     AVLYRDALRHVRQTEQFDFVEVSWLLETNALIISAGESFAARHYKTWRIYEAPL"
     gene            complement(4217..>4582)
                     /locus_tag="PKW60_09685"
     CDS             complement(4217..>4582)
                     /locus_tag="PKW60_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPJ99743.1"
                     /translation="KGMNVTQDMITANPNLAGIFAANEGGAIGAAQALRADGKAGEIK
                     LVAFDASDEEIAALKEGVIQALVVQNPYKMGYEGVKAAVDHLEGRPVEKRIDTGVTVV
                     SMENFDEPEIQNILNPLSK"
BASE COUNT          830 a         1371 c         1490 g          891 t
ORIGIN      
        1 attgaggccg atggagaggc cgtggaatcg atgcacccgt tttgccggat cgatcgcccc
       61 gttccaattc tggccccgcc cgtaggggcg gcgattgatg cgccagagcc ctatgccatg
      121 ctggccatgc gcgacctgcc cctctacacg gtgtgtttcg ccgccgataa ccccgagctc
      181 gacaccctgg tcgacacggc gatccagagt ggactgcgcc ccgtgatcgt cgtggatttg
      241 gccgtgacgc ctctggacgc cctgtccgga ctcctggagc gctacggttc gcgggcggcg
      301 cactggacag tgctttccgg cggcgctccc ggtgtacagg agcctgccgc ggcgctgttc
      361 gccggccacg agccggctgt gccggtttcg tgggcagccg gtacgcccga ggaactgacc
      421 gccctgctgc aatccagcgc cgggcagcct gtctccgcag tggttttcca gcacaaggcg
      481 ccgtcggcgg aggagctggc cgcctttgag cttgcggcgc aggcagccgg ttacgaacac
      541 cttcccatcc acatcctcgg ccgcggaatc gcagaagacg gtccggatgc agccgcgcgc
      601 ctggcgtgta atttaatcga cttcgccgcg aggggcgtgg ccgaagtgaa catcgacggc
      661 gcgctgttgt tccatgatca ggaagtcacc gaggcctatg tgttcgtgag tgcgctggta
      721 cgcagcctgg ccggtgcggt gcctatcggc atgctcgacg ctcccgcgcc gtaccgggcg
      781 gttgtgttcc ggctcggtga ctcgtggctg atcgccgcct ggagcgaagg gaaggccggc
      841 agccttacta ttccggtggg cgaagccgtc cttcacgaac tggcggatgc ctcgaacaat
      901 ccgctggaac tcccggccgt gactgaagag gcggtgtctc tggagctggc ggcggcgccc
      961 gtgtatcttc gtggcgaagg agggacgatt ctggcctcta cagcgcgccg catgatgcag
     1021 aacgccgccg cagactttgc ggccaatccg gcctacgccg ccctgttgcc ggagcaggtc
     1081 gcggacatct tcgccatcat gcggaaggct gttccgccgc gcatcgagcg cagccagttt
     1141 cttgctctgc tcccgatgtt tccgctcctg gaggcgcaat ggcattcagg caaggtgccc
     1201 cgggaggttg ccgtgcccgc catgagcgcc ctgtcggaca ttattcgcca tgcggcggtg
     1261 atcgaacagg cgttcgacga gccgtttgtg cagccgcttc aagacacgct ggccacctgc
     1321 acagagttcc agcaggattt cctgacgcgt gcggaagcac cgcctgagac gcgtgcgcgg
     1381 gccgactggc tgctcgccga agtgacgcgg ctggcggaag aggcgaagca tctcaatgaa
     1441 acagacagga ccatcgaggc caacgccata gcgtcgctgg cgaaatggcg cgcacacgca
     1501 ttgaacgcga ctctgaacta cgtggcgcct gcggaagagg ccgcggtaga atccagctcg
     1561 ggcacgtcct gaacaaggaa acagatatga agcggatagg agtgttgaca agcggtggag
     1621 atgcgccggg catgaacgcg gcgatccggg ctgtggtgcg cgcgggtgcg tactatgact
     1681 tgcaggtctt cggcatccag cggggttacc aggggctgat cgacggccat gtgtttgaaa
     1741 tggggccgcg gtccgtcagc gggatcatca accggggggg aacgatcctg cgcacggccc
     1801 ggtgccaggc ctttttcgag aaagacggcc gcacccaggc ggccgacacg ctccggaaac
     1861 accacatcga tgggctggtc gtgatcggag gcgacggttc gtaccggggc gcactggccc
     1921 tgtacgaaga gcacggtatc gcctgtatcg gcctgcccgg aaccatcgac aacgacattg
     1981 gcggaaccga ttacagcatc ggctacgata cggcgctgaa cgtggcgctg gaagcgatcg
     2041 accgcatccg cgacaccgcg gaatcccatg aacgcatctt tttcgtggag gtgatgggcc
     2101 ggaacagcgg ctacctggcc atgatgagcg gcatcgccgg cggcgccgag gagatcctcg
     2161 tgccggagac gccgaccagc atagaaaccc tcgttgaaca tatacacgcc taccgggcca
     2221 agggaaagca gtcggccatt atcgtggtcg cggaagggga cgagctgggt gatgcgatcc
     2281 aggtcgccaa acaggtggat gccctggcca agatccccaa cagccgggtc gtggtgatcg
     2341 gccatctgca acgcggaggc acgccgacgg cgtttgaccg tatcctggcc agccgcctgg
     2401 gcgtgcgtgc cgtggaagcg ctgcgcgaag gcgtgaccgg gaaaatggtg ggcatttcaa
     2461 cggacggcgt tgtgctgcgt ccgctgcagg aggcttggga atgccgcacg cagttcgacc
     2521 cgaaactggc gcgaatcgcc cacattctcg catcatagcc ccggagccgc cgcggctggc
     2581 ctgaggggcc tgctcattac agaggtgctt cgtaaatccg ccaggttttg tagtgccggg
     2641 cggcaaacga ttcgccggcg ctgatgatga gggcattggt ttccagcagc caggagacct
     2701 cgacaaaatc aaattgctcc gtctggcgaa catgacgcag ggcgtcgcgg tacaacacgg
     2761 catcgagtcc ggagcggcgg tacggttctt tgatgccaag cagggtgacc cgcccgcgcc
     2821 gggtgcggtg cttgttgcgg aacagccggg ccacacgcac aaaatccagc ctgccgaacg
     2881 cgctgccgtt ggtcttgatg cagtcgtaga tgtcgggcag gatcaggaaa aacgctgccg
     2941 cttcatcatg gacatatccg aggagcgcga tccctgattt gacgatcgta cggagcttat
     3001 cggcggtcag gttgaattcc tcttccgtga aggccgtcgc gccccagtta tctttccagg
     3061 cgtcgttata aatagagcgg atgagctttc tttcttccgc gaggttggag aagtcgaggg
     3121 ggcgcgtcca caggccgttg cggcgggcca tgcgctcggc gatcttgttg atgcgttcca
     3181 tgagccgggg atcggccgcg ccgttggtaa aatccagttt ttgggcgatg acgtccatgg
     3241 ctttgtacat gccgtagcgg tccagcaagt cagcgtaata ggggtaattg ctcgcggtct
     3301 cataatacgg cagcgtgtca aaggcgttga gctggattcc gacgatttcg ttggtagaga
     3361 aactcatggg gccgaggagg gtggtcatgc cccgtgcctt gccccaggcc gccactcgat
     3421 ccagcagcgc cctggcaacc tcaaactcgt tgatcacctc ccagaatccg aaaatgaccg
     3481 ccctggcgtt ctgctgctcg ttatagcgct ggttgataca acccgcgatg cgtcccacga
     3541 ctttgccgtc gcgttccgcg aggaaataca ccacctcggc atgcttgtgg aaggggtgtt
     3601 tgtccgtgag caggcctttt tcgcccagga gccggttgcc gagcagatcc atgttcagcg
     3661 ggggcacgta gtacgggtgg tccttgtagt ggtcccaatg gaactggacg aatcttttca
     3721 gtagcctgta atttccgggt ccgaactcga taaggttgat caacagaaat ctccccggaa
     3781 cctcaacgca tggttgatgg gagggaaaat gctgcggggg gacgcgcgtc gagcagtaca
     3841 gcctgcgctg tccgccgtct acagcctttc agcaccgtat gcctgccaca tactaccccg
     3901 catcggcgcc ttcgttaccg tatacgcccc gccacgccgc cagactcccc gaggagcctg
     3961 gtcgcgtgcc ctcatttttc cagttgctgc ctggccgaca ccgaatagaa gcggtatggt
     4021 cgcccatccg gcaggggaat gtcaaggaca agcagctcac gaatttttat tgaattcttc
     4081 aggggaggtg cgggaaaaga gtgccaacgg cgtttttctg caggccactg gagccgtgtg
     4141 cccggctggc gcccgggatt cacagcccag cgcctgattc gtgtatggat aggccggcaa
     4201 acccggacag cctatgttac ttgctcagcg gattgaggat gttttggatc tcgggctcgt
     4261 cgaagttttc catgctgacg acggtcacgc cggtgtcgat acgtttttcg acggggcggc
     4321 cttccaggtg gtccacggcg gctttcacgc cttcgtatcc catcttatag ggattttgaa
     4381 cgacgagcgc ctggatgacg ccttccttga gcgcggcgat ttcttcgtcg gaggcatcaa
     4441 acgccacaag cttgatctcg ccggccttgc catcggcgcg cagggcctgt gctgccccga
     4501 tggcgccgcc ttcgttggcc gcgaagatgc ccgcgagatt gggattggcg gtgatcatgt
     4561 cctgcgttac gttcatgccc tt
//
LOCUS       DAOPBQ010000287         4577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15655_length_4577_cov_3.765148, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000287 DAOPBQ010000000
VERSION     DAOPBQ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15655_length_4577_cov_3.765148"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..979)
                     /locus_tag="PKW60_09690"
     CDS             complement(11..979)
                     /locus_tag="PKW60_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013113187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPJ99744.1"
                     /translation="MPNIDSKKKLALVEAAIESVRELCRACRLCGRVCGVNRLENEPG
                     FCATDSRDAEHVRYASTTLHFGEEPPLVGRGGSGTVFFTHCNLRCVFCQNYQISQFGL
                     GDDTGYAVLAQAMLALERAGAENINLVTPTHYIYPILLALREAYRRGLARPLVYNTNA
                     FDRVELLALLDGIVDIYLPDMKYSDQSCAQTYSQAAQYPGIARAAILEMYRQTGRLQH
                     RDGVATKGLIIRHLVLPEDQAGSYDFLLWLKDEGLMDVTLSLMRQYSPRHQAMRYERI
                     ARVVTGKEYLDVVRYARQLGFEELLVQGVESPDVYLPDFTQDDPFS"
     gene            complement(1033..3840)
                     /locus_tag="PKW60_09695"
     CDS             complement(1033..3840)
                     /locus_tag="PKW60_09695"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPJ99745.1"
                     /translation="MSRGYEIHVIANTHWDREWLYNFQETRLMLVEFLDLLLEVLDTE
                     PDYKSFLMDGQCVPIEDYLEIRPQHRARIERHVSDGRLQIGPWYTLPECFSVNGESLV
                     RNLIYGHRVSKEFGGVMKVGHTAFSYGQNSQMPQIYQGFGIDVMLFYHGVSHDEVANE
                     FIFEAADGTRILGSQMSSGARYNFYHNVYRPAVFGKTIAERMYSWQEGGLPFHLCTEE
                     RAAGHHLLLNPARGFDRQRARECLAALRDKEAAVATTRFLAFMMGHDSSIPDLAELEL
                     IQEARKVLKDDTILHSRLPDLMEKVKKAVKNLTVLKGERRTPVLMNQRMHLYSDVLSS
                     RTRVKRLNALTEQALQRRAEPFAALAWGLGADYPAAPLDLAWKTLLKCHPHDSISGSG
                     VDDIERDVRDRLRQAANLSDSILSRSLQHIQRRIDNSKLGVDELVLTVFNASPRTRSE
                     VLTAVVDIPRTSAMSAFEVAACGPLPAGARKPAAAQVVTRKPHHAVINHAADATYMLE
                     CEQVKFHFEARDIPAFGYGTFRLVPSKTFGRGSLVRGYHSMENEHLAVYINGDGTLTL
                     EHKESGEVYDGLHFFEDGGEVGNAWMHIEPAADRIVSSIGMPVTIMLEEDGPLLARFR
                     IEYHMTIPASFEEGLGDAWQRLDGGGSASRRSEETVPLKITSWITLRRGARSVDITTR
                     FTNTARYHRLRVLFPTRRQTKTCHVETPFDVVKRPTTHGSDSPWQASNATFPMQRFVD
                     VADRRGGLAVINDGLREYEITPDDDVAVALTLVRAYEIALTTVSKRWERHPEMELSQC
                     PGEHEFRYSIYPHAGSWEEAGVYEEVERLSVPLEIAQAGPHGGDLPQRLGLLSVSPSN
                     AVVSALKQSEDGAAVVLRLFNPTTKALPVEIKTHAKILAAHLLTLEELPAKKLAPAGK
                     SVKFSLAPKKIETVKLTLAAL"
     gene            4107..>4577
                     /locus_tag="PKW60_09700"
     CDS             4107..>4577
                     /locus_tag="PKW60_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99746.1"
                     /translation="MTIRRSLLGIIGLIGTLAICGGTVSAEVPHVKIGRFPQEVASVF
                     TTADGLPSDDVRAVCLDAQGAVYAQTAAGWAVFQDGRWTAVDAPDTSAFPGCVDPARY
                     AQLGDSARQVAAGPDGQLVAATSAGLMRQTAAGFEPLVVEDGLGRQWGTSDVRGV"
BASE COUNT          850 a         1434 c         1367 g          926 t
ORIGIN      
        1 ctcgcaggat ctacgaaaaa gggtcgtcct gtgtgaaatc gggcaggtag acgtcaggac
       61 tctccacgcc ctgaacgaga agctcttcaa agccgagctg gcgcgcatat cgcacgacgt
      121 ccagatactc tttcccggtg accactcgcg cgatgcgctc ataacgcatg gcctggtgcc
      181 gcggcgaata ctgacgcatc agactcaatg tcacgtccat gagcccctcg tccttcaacc
      241 agagcaggaa atcgtagctg ccggcctggt cctcgggcag cacgaggtgg cgaatgatga
      301 gcccctttgt ggccacgccg tccctgtgtt gcaggcggcc cgtctgccgg tacatttcca
      361 gtatggccgc tcgtgcaatc ccaggatatt gcgccgcctg ggagtaggtc tgcgcgcagc
      421 tttggtcgga atatttcatg tccggcaggt agatatccac gatcccgtcc agcagggcaa
      481 gcagttcgac gcgatcaaat gcgttggtgt tgtatacgag cggccgtgcc aatccccggc
      541 ggtaggcttc tcgtaacgcc agcaggatcg ggtagatata atgcgtcggc gtcaccaggt
      601 tgatgttttc cgcgcctgcc ctctccagtg cgagcatggc ctgagccagc accgcatagc
      661 cggtgtcatc gcccaggccg aactggctta tctgatagtt ttgacagaat acgcagcgca
      721 ggttgcagtg cgtgaaaaac accgtgcccg agccgccacg ccccaccagc ggcggctctt
      781 caccaaagtg cagcgttgtc gacgcatacc gcacatgttc ggcatcgcgg gagtccgttg
      841 cgcagaaacc cggctcgttt tcgagccggt tcacgccgca gacccttccg cacagacggc
      901 aggctcggca cagctcccgc acggactcga tcgctgcctc cacgagcgcc agctttttct
      961 tcgaatctat gttcggcaca gacgatttcc ctcgcgcgca aggcggtagc cgcgcgcggg
     1021 cgcacctctg ctctaaagag ctgccagtgt aagctttaca gtctcaatct tcttgggagc
     1081 cagcgagaat tttacggatt tccccgcggg cgcaagtttc tttgcgggca gctcttccag
     1141 ggtcagaaga tgggctgcga ggatctttgc atgggtcttg atctccacgg gcagggcctt
     1201 tgtcgtggga ttgaacaacc gcaacacgac cgccgcacca tcttcagact gcttcaacgc
     1261 actgacaact gcattcgatg gcgatacgct cagcaacccg agccgctgcg gcagatcgcc
     1321 cccatgaggg cccgcctggg caatttccag cggtacgctc aggcgttcca cttcctcgta
     1381 gacgccggcc tcttcccagg aaccggcatg cggatagatc gaatatcgga actcatgttc
     1441 tcccggacac tgcgacagct ccatctccgg atgccgctcc cagcgtttgc tgacagtcgt
     1501 caacgcgatc tcgtacgcgc ggacgagcgt cagcgcgacg gccacatcat cgtccggcgt
     1561 gatctcgtat tcgcgcaggc catcgttgat gaccgccaat ccgccgcggc ggtcggccac
     1621 gtccacgaag cgctgcatcg ggaacgtcgc attggatgcc tgccacgggc tgtcggaccc
     1681 gtgcgtggtc ggacgtttga ccacatcgaa aggagtctcc acatggcagg tcttggtttg
     1741 ccgccgtgtc ggaaacagca cgcgcagacg gtgatagcgc gctgtgttgg tgaaccgcgt
     1801 cgtgatgtct accgaccggg cgccccggcg cagcgtaatc cacgacgtga ttttcagcgg
     1861 cacggtctcc tcgctgcgcc gcgatgcgct gccgcccccg tcaagccgtt gccacgcatc
     1921 tcccaggcct tcctcgaagg aggccggaat ggtcatatgg tactcgatgc ggaagcgtgc
     1981 cagcagcggg ccatcttctt ccagcatgat cgttacgggc atcccgatgc tcgaaacgat
     2041 acgatccgcg gccggttcga tatgcatcca cgcattgccg acctcgccgc cgtcctcgaa
     2101 gaaatggagc ccgtcgtata cctcgcccga ttccttgtgc tccagggtga gcgtgccgtc
     2161 tccgttgata tagacggcca ggtgttcgtt ctccatcgag tggtagcccc gcaccagact
     2221 tccacggccg aacgtctttg acggaacgag acggaaggtg ccgtatccga acgcgggaat
     2281 gtcgcgggct tcgaagtgaa acttcacctg ctcgcattcg agcatatacg ttgcgtcggc
     2341 cgcgtggttg atcaccgcat gatgcggctt gcgcgtgacc acctgtgctg ccgcgggttt
     2401 gcgggctcca gcgggaagcg gtccgcaggc ggcgacctca aaggcgctca tggcgctggt
     2461 gcgggggatg tctaccaccg cggtcaagac ttcggagcgt gtccggggcg acgcattgaa
     2521 cacggtgagc accagctcgt ccactcccag tttcgaattg tcgatgcggc gctgaatgtg
     2581 ctggaggctt ctcgacagaa tggagtccga cagattggcg gcctgccgga ggcggtcgcg
     2641 cacgtcgcgc tcgatgtcat cgacgccact gcccgagatg ctgtcgtgcg gatggcattt
     2701 caacagcgtt ttccaggcga gatcgagcgg cgcggcggga taatcggctc cgagtcccca
     2761 ggccagcgcc gcaaacggtt ctgcccggcg ctgcagtgcc tgttccgtca gagcattcag
     2821 gcgtttgacg cgcgtgcggc tcgagagcac atcgctgtac agatgcatcc gctggttcat
     2881 cagcacgggc gttcggcgtt ctcctttgag caccgtgagg ttcttaacgg ctttcttcac
     2941 cttttccatc aggtcaggca gccggctgtg caggatcgta tcgtctttga gcactttgcg
     3001 ggcttcctgg atgagttcca gttccgccag gtccggaatg ctgctgtcgt gacccatcat
     3061 gaacgccaga aagcgcgttg tcgccacggc tgcttcctta tcccgaagtg cggcgaggca
     3121 ctcgcgcgcc cgttgccgat caaatccccg cgccggattc agaagcaggt gatggcccgc
     3181 agcgcgctct tcggtacaca aatggaaggg cagcccgcct tcctgccacg aatacatgcg
     3241 ctcggcaatc gtcttgccaa acacggccgg ccggtacacg ttgtggtaga agttgtagcg
     3301 cgcgccggaa ctcatctgcg accccaggat gcgcgtcccg tccgcggctt caaaaatgaa
     3361 ctcgttggcc acctcatcat gggatactcc gtggtaaaag agcatcacgt caatcccaaa
     3421 tccctgatag atctgcggca tctgtgagtt ctggccatac gaaaacgccg tgtgccccac
     3481 cttcatcacg ccgccgaatt ccttcgaaac ccggtgtccg tagatcaggt tgcgaaccag
     3541 agactccccg tttacgctga agcactccgg cagggtatac caggggccga tctgcaggcg
     3601 gccatctgaa acatggcgct caatacgcgc gcgatgctgc ggccggattt cgaggtagtc
     3661 ctcgatgggc acgcactggc cgtccattag aaatgattta tagtccggct cggtgtcgag
     3721 cacttccagc aggaggtcca ggaactcgac cagcatcagc cgcgtttcct gaaaattgta
     3781 cagccactcc cggtcccagt gcgtattcgc aatgacgtga atctcatagc ctcggctcat
     3841 cggcgctctc ccctgatgta aacgaaccct ggatgttgac gggattctac gacaatatgc
     3901 tgccgccgac aaagtccggc cattggcgct gtctccgcat attgataatc cccacggatt
     3961 ccgggtataa agtcttgtcg agcgatgtga attccagcac aggaggcctc catcgccgtt
     4021 cagcaaggcg cacccgcccc aaggtatttc cagcacctcc gtgggcatgc ccagccaggc
     4081 aatggaataa atgatgaggt aggatcatga ccatccggcg cagtctcttg gggataatcg
     4141 ggttgatagg aacgctggcc atctgcgggg gaaccgtgag cgctgaagtg ccgcacgtca
     4201 aaatcggccg tttcccgcag gaggtcgctt cggtgttcac gacggcggac gggctccctt
     4261 cggatgatgt gcgtgccgtc tgcctcgatg cccagggcgc cgtctatgcc caaaccgctg
     4321 cgggatgggc ggtttttcag gatggccgct ggacggcggt cgatgccccg gacaccagcg
     4381 cattccccgg ctgtgttgac ccggcgcgct acgctcaatt gggtgattcg gcccggcagg
     4441 ttgccgcggg accggacggt cagctcgtcg cagcgacatc cgcgggactc atgcgccaaa
     4501 cggccgccgg ttttgaaccc ctggttgtgg aggacggcct gggccgccaa tggggcacct
     4561 cggatgtccg cggggtg
//
LOCUS       DAOPBQ010000288         4561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15719_length_4561_cov_3.413227, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000288 DAOPBQ010000000
VERSION     DAOPBQ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15719_length_4561_cov_3.413227"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKW60_09705"
     CDS             <1..540
                     /locus_tag="PKW60_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPJ99747.1"
                     /translation="HLGNGLAAELDRHSNPLWPQLAEDRLAAAIIADLHHVPPDMLKT
                     MVRMKGSDNVILTSDAVRIAGLKPGMYPHHSGVKGMMVELLPSGRINLCGTNLLSGSA
                     LMLLQGVLNATRETDMTLEQSFRCASMIPAEILGVKADFGTLKPGAVANLIVFDLDQS
                     HPQWKPVIRAAFVHGVQAA"
     gene            797..1210
                     /locus_tag="PKW60_09710"
     CDS             797..1210
                     /locus_tag="PKW60_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPJ99748.1"
                     /translation="MNISSRCEYACRAIIELALNERTEQPLTSVSIAERRKIPVKYLV
                     HILVQLKRAGLVRSVRGAQGGYLLSRSPDDITLLDLIEAIDGPILDPLPINDDGGAEL
                     NRAWENAARQLSRVMREYRIRDIIDGTGRPDMYYI"
     gene            1531..2913
                     /locus_tag="PKW60_09715"
     CDS             1531..2913
                     /locus_tag="PKW60_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPJ99749.1"
                     /translation="MRRNKNKMNRREALRVVSGAVAGWGWARWASAIEMPEVKIPRAT
                     DGDLVVEPDWEQRLVITVGPSGADLVGESEKTIQAAVDYVARLGGGTVHIQAGTYRLR
                     NAIVLASGVRLLGDGDKTVLIKEPSSQTTIAEDSDWFDQEITLADATGFEVGDGICIQ
                     SDNPHHSGKVILKRTLVARSGNRFKLDRGLRENIWTMKTPTVSTLFPLVTAEEKSNLA
                     IENLVIDGNREANAHLDGNYAGNLWFQDCSRILIRGVETRNNNGDGISWQICHDVVVE
                     DCYVHDNADLGMHPGSGAQRPLIRNNRIERNTIGLFFCWGVQYGVAENNRMVDNSGQG
                     LSIGHRDHYNIIRNNEITGSGQTGVLFRPERGEGFTATGNLLEENRIYDNGPENGAAV
                     DFQGVTAGNTLVRNEIRETRGPAERVGSESVKNAATTPSPTITSTGSQSPWRTSDRSK
                     RPSRASALRL"
     gene            complement(3392..>4561)
                     /locus_tag="PKW60_09720"
     CDS             complement(3392..>4561)
                     /locus_tag="PKW60_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99750.1"
                     /translation="LRDTTSIETSCAESRALRQRAWRQQSSFLRSQRTWGLSMVRRRK
                     VHSTKGRGSLAAAGLMVFGFMATMAPPENPCEYPSARELFARIQTELEAEPLQVLLEE
                     WDQLVASRDFRERDKALWLVATHVKARLFFGAGTAIKYSNEEIKARVVELYLDECARM
                     DASPMEGDKESDPSYGGEAGWEYLSDLMTVAESTFDPRLYEYEVRIMPAATGRLRESY
                     LAVVNPDKTLHIILDAHLGYRDGKKMNQDSLYFTKEGMSASFLLDGAFRVMALMKKES
                     PESLRKQKERVVEFVRVHALHFSAPRKEVYRDEPVRLCWHDFYCRGGAFDVLELLGDS
                     RDLPFAEKLSQDLPDMPEKDFEESPRHYLKRESLTEKARRVIPLLRSREPSEVQQ"
BASE COUNT          976 a         1473 c         1270 g          842 t
ORIGIN      
        1 cacctcggaa acggccttgc cgccgaactg gaccgccaca gcaaccccct ctggccccag
       61 ctggccgagg accggctcgc cgcggcgatc atcgccgacc tccaccacgt tccacccgac
      121 atgctgaaga ccatggtccg catgaaaggg tccgacaacg tcattctcac ctcggacgcc
      181 gtccgcatcg ccggtttgaa accgggcatg tatccgcacc acagcggcgt caagggaatg
      241 atggtcgaat tgctgccgtc gggacgcatc aacctgtgcg gcaccaatct cctctccggc
      301 agcgccctga tgctgttgca gggcgtcctc aacgccacgc gtgaaaccga catgacgctc
      361 gaacaatcgt tccggtgcgc gtccatgatt ccggcggaga tactgggcgt caaggccgat
      421 ttcggcacgc tgaaacccgg cgccgtcgcc aacctgatcg ttttcgatct cgaccagtcc
      481 catccccagt ggaaacccgt catccgcgcc gcctttgttc acggcgtgca ggccgcatag
      541 cagggtgcca tctcgttaac gccgtgtggg actgctgcga tcggcagccc gttggatgca
      601 gaaccctgac accgaaaaga caggaatccg tgctctttac ttctcccatc aaagctagta
      661 cgatatgagt gtccagaata gagagttcgc ggcggatgct cgggggggca gggtttgcgc
      721 ggcgccgcag acgcgtccgc gcgagggcgc ctgaggagat gggggcggcg cccggagaag
      781 gaaggcgaac tgccgaatga atatctcgtc caggtgtgaa tatgcctgcc gggcgataat
      841 cgaactagca ctgaacgaac gaactgagca gccgctcacc tcggtttcca ttgcggagag
      901 gcgcaagata ccggtcaagt atcttgtcca tatccttgtg caactgaagc gggcaggact
      961 ggttcgcagt gtccgcgggg cgcagggcgg ctacctgctc tcccgctcgc ccgacgacat
     1021 cacgcttctg gacctcatcg aagccatcga cggcccgatc ctcgatcccc tgcccatcaa
     1081 cgatgatgga ggagcggagc tcaaccgcgc atgggaaaac gcggcgcgcc aactgtccag
     1141 ggtcatgcgg gaatacagaa ttcgcgatat tatcgatggc accggccgac cggatatgta
     1201 ctacatctga ccggtgaccg gccgccgtat cccctgctgt gcatggcacc tccgccgctg
     1261 tagtcaatct ttgaaatcca cccccgaatc ggctggcgcc gattcacccc cgccagctgg
     1321 ggacaagtgc gccgcacccg aattgtaagg gcggccccct gtggcagccc atccggatag
     1381 ctctacgcgt gcgtccatcc tcacgcgttg accttgatcc agggcaggca cgggggcctg
     1441 cccctgcaca tgcgcgccgt ttttgcacca cggcacgcta ttgtgcacta tagggcctcg
     1501 caggaaggcc gttttcaagg agactacact atgaggcgga acaaaaataa gatgaatcgg
     1561 cgcgaggcgc tgcgggttgt ctcaggagct gtcgcgggat ggggctgggc ccggtgggcc
     1621 agcgcgatag agatgccgga ggtgaagatc ccgcgcgcca cagacggaga cctcgtcgtg
     1681 gagccggatt gggagcagcg cctggtgatc accgtgggcc cgagcggcgc cgatctcgtc
     1741 ggggaatccg agaaaacgat tcaagcggcc gtggactacg tggcgcgcct tggcggcggc
     1801 accgtacata ttcaggctgg cacctaccgc ctgcgaaatg ccatcgtcct cgcttcaggc
     1861 gtccgcctgt tgggcgatgg cgacaagacc gtccttatca aagaaccatc ctcacaaacc
     1921 accatcgcgg aggattccga ctggttcgac caggagatca ccctggccga cgccacgggc
     1981 tttgaggtcg gcgacggcat ctgcatccag agcgacaacc cgcaccacag cggcaaagtg
     2041 atcctcaaac gcaccctcgt agcacggagc ggcaaccgct tcaaactcga ccggggactg
     2101 cgcgaaaaca tctggaccat gaagacgccc accgtctcaa ccctgttccc cctggtcacc
     2161 gccgaggaaa aatcgaatct tgcgatcgaa aacctggtca tcgacggcaa ccgggaggca
     2221 aatgcacacc tcgacggcaa ctatgcggga aatctctggt ttcaggattg cagccgcatc
     2281 ctcatccggg gcgtcgagac ccgtaacaac aacggcgacg gcatcagctg gcagatatgc
     2341 cacgacgtcg tggtcgagga ctgctacgtc cacgacaacg ccgacctggg catgcatccc
     2401 ggttcagggg cccagcggcc gctcatccgc aacaaccgca tcgaacgcaa tacgatcggg
     2461 ctgttcttct gctggggtgt gcagtacggc gtcgcggaaa acaaccggat ggtcgacaac
     2521 agcggccagg gcctctctat cggccaccgc gaccactaca acatcatccg aaacaacgag
     2581 atcacgggca gcggtcaaac gggcgtgctg ttccgccccg aacgcggcga aggattcacg
     2641 gccaccggaa atctcctcga agaaaaccgt atctacgaca acggtcccga aaacggcgcc
     2701 gcggtcgatt tccagggcgt caccgctgga aacacgctgg tacgaaacga aatccgcgaa
     2761 acgcgcggcc ccgcggagcg cgtcggatcc gaatcggtga agaatgcggc gacaacacct
     2821 tcaccgacaa tcacatcgac gggttcgcaa tccccatgga ggacaagcga caggtctaaa
     2881 cgcccgtccc gcgcatccgc cctccggctg taggggcggc cccctgtggc cgcccatccg
     2941 ggtagcccct gcggctgcct tgaagaaggg cagatacaga ggtctgcccc tacgcgctgc
     3001 gtagcataga taaaccagga tagccccagc gcgcgcgtaa ataaccccga cggcagcggc
     3061 accgtctcga agcccggcgg aaccacgaca caggccacaa acgcacactg cccttcgccc
     3121 gcggccgagg ccagcgtcag cgtcgttttc ggcagatacg cgctgtccga cccggtggcc
     3181 attcccgtca ggatcagatg ctcgaccgaa cccagccgat acaccacatc ctcataaatg
     3241 aattcatccg gaccgagccc gtcaggcaca tcccacccgt ccgattcccg gtccatcgct
     3301 gccgcgacga acagggccac gacgcttcaa ggcctcggca gtgtgcaaag acatgcagat
     3361 tgagcttctg tcttagccag aacgatggct ctcattgttg tacctcactt ggctctctgg
     3421 aacgtaacag cgggataaca cggcgtgcct tttcggtgag tgattcccgc ttgagataat
     3481 gtcgcgggga ttcttcaaag tccttttcgg gcatatcggg caaatcctgg gacagcttct
     3541 ccgcgaaagg caggtcgcga ctgtccccga gtagttccag cacgtcgaaa gcgcctcccc
     3601 ggcagtagaa atcgtgccag caaagccgta cgggttcatc ccgatagacc tccttccgcg
     3661 gggcggagaa atgaagagcg tgcacccgca caaactcaac aactcgttcc ttctgtttcc
     3721 ggagcgattc cggagattct ttcttcatca gagccataac tcgaaatgcc ccgtccagca
     3781 agaagcttgc cgacattcct tcttttgtga agtagaggga atcttgattc atttttttgc
     3841 cgtcgcgata tcccaggtga gcatcaagta tgatatgcag cgttttatca ggatttacga
     3901 cggccagata ggattccctc agacgaccag tagctgctgg cataatccta acctcgtatt
     3961 cataaagccg cggatcgaac gtggattctg caacagtcat cagatccgat agatactccc
     4021 aaccggcctc cccgccatac gatgggtccg attccttgtc tccttccatc ggggaagcgt
     4081 ccattcttgc gcactcgtca agatatagtt cgacaactcg cgctttgatt tcctcattgc
     4141 tgtacttgat ggctgtaccc gcgccgaaaa acagtcttgc tttcacgtgt gttgctacca
     4201 gccacaatgc cttgtctcgc tctctgaaat cgcggctggc cacgagctgg tcccactctt
     4261 ccaataatac ctgcaaaggt tccgcctcaa gctctgtctg gatccgcgca aatagctccc
     4321 tggcagatgg gtattcacac gggttttccg gtggcgccat ggtagccatg aacccgaaca
     4381 ccatcagccc cgccgcagca agcgacccac gtccttttgt cgagtgcact tttctccttc
     4441 tcaccattga tagcccccac gttctttgcg accgcaaaaa tgaactctgc tgcctccatg
     4501 ctcgctgcct cagcgcacgg ctctccgcgc acgaggtctc gatcgaggtg gtgtcgcgca
     4561 g
//
LOCUS       DAOPBQ010000289         4542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15799_length_4542_cov_4.423000, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000289 DAOPBQ010000000
VERSION     DAOPBQ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15799_length_4542_cov_4.423000"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1174)
                     /locus_tag="PKW60_09725"
     CDS             complement(182..1174)
                     /locus_tag="PKW60_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99751.1"
                     /translation="MHKVGIIGLGFIGKMHLGSLRRTGMAEIVAVADKNPANLGKGAV
                     QGGNIALEGDLSLDGVKTYSDGDELLADPDVEIALVTLPTYLHKEYVLKAIKAGKHVM
                     CEKPFVRSSDEGREILAALEGYDKNLMVLQCIRFWPAYAKAYDYVKSGEYGKVLSAHF
                     VRQSPKPTWSWDNWLMDNPRGGGALVDLHIHDVDFVNYCFGKPDSIQAAGVNVPGEGV
                     GDIMALYTYQGGPVVTLHGAWNYMPKFPFRMAFTMSLEKASFEFNSMVDMNLRVYPKD
                     GGELVPELDPADGYLLEEKYFLECIDKGVKPSIVTPEDALESVVLAERELAAMA"
     gene            complement(1220..2086)
                     /locus_tag="PKW60_09730"
     CDS             complement(1220..2086)
                     /locus_tag="PKW60_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPJ99752.1"
                     /translation="MKISVSGWTVGGFNNETPVLEAAKLAQDAGFDAIELCFGAGELS
                     PETSDAALTAMREGIEGMGFAISSLATGVYWAKSLSSPDEPERKEAVEFTKAYINAAS
                     VLGVDAILVVPGAVNVPFDPSRPVVPSKTVWDISQRSIKELLPFAEKAGVAICVENVW
                     NKFLTGPFELAQYIDSFQSPFVKSYFDAGNVLLTGYPEHWIEVLGGKRIGRVHVKNFT
                     AHDGGGTLADFTDSLMKGTLNWEAVFGALKNVGYDGYITPEMLVSEKGLPDEELARQV
                     AKDMRRLLEKYG"
     gene            complement(2480..3241)
                     /locus_tag="PKW60_09735"
     CDS             complement(2480..3241)
                     /locus_tag="PKW60_09735"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPJ99753.1"
                     /translation="MDLGRIEEYYLDARQYDAENDDYVLDIPFYVERAKRAGGPVLEI
                     ACGTGRVTLPIAREGIEVVGLDVAPAMLARLRDKAAHEDLPISLVEADCRDFALGRQF
                     TLIICPFSAMQHLHDLPSLESFLERVQTHLKADGLFIFDVFNPNVEVLSSGAGARARI
                     FTYTDKESGADVFVEETTDYDDAAQVLHITWYSTGGGTDEIREETIHLRCFYPQELDM
                     ILRYNRFEILEKLGKFSGEPFEAGLGRQIVVCRPV"
     gene            complement(3372..3956)
                     /locus_tag="PKW60_09740"
     CDS             complement(3372..3956)
                     /locus_tag="PKW60_09740"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPJ99754.1"
                     /translation="MAFTLPSLPFSRSALEPIISAETLEFHYGKHHQAYVNNLNALVE
                     GKPEAKMPLDELIMKAEGGVFNNAAQVWNHTFYWNCLKPGGGGEPSGALAEAIDRDFG
                     SFDAFKEEFSGAAAKLFGSGWAWLVLDDGKLKIAQTSNADLPMKHGQTALLTIDVWEH
                     AYYIDYRNARPKYIEAVLDNLINWDFVEQNLTRG"
     gene            complement(4201..>4542)
                     /locus_tag="PKW60_09745"
     CDS             complement(4201..>4542)
                     /locus_tag="PKW60_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPJ99755.1"
                     /translation="QMVGMMLHQLGYRVDAFTNSLAAFEAFCANPDSYDLVIVDQIMP
                     HLMGVELAKRILETRPGFPVFLLTGYSEGITPEQARELGICEFLMKPFSSRELGGLIR
                     KTLDTVVPERN"
BASE COUNT          900 a         1444 c         1262 g          936 t
ORIGIN      
        1 ttcagaagac atgatctccc ccattccacc cctgcttcag agatactata tcgttgcgga
       61 acgaaagaag aaagggcaga ggctcaatcc tgcgcgactg cgaacccgcc gcgccacaaa
      121 aaacgcccgg agaagagtcc tccgggcgtt gctggggccg gtgtctcggc atgtcgcagg
      181 gtcaggccat cgccgcaagt tcccgctccg ccagcacgac ggattcgaga gcatcctcag
      241 gcgtcacgat ggacggcttg acgcccttgt cgatgcattc gagaaagtac ttctcctcga
      301 gcaggtagcc atccgcggga tcgagttcgg gaaccagctc gccgccgtcc ttggggtaaa
      361 cgcgcaggtt catatccacc atcgagttaa actcgaacga tgctttttcg agcgacattg
      421 taaacgccat ccggaagggg aatttcggca tgtagttcca ggcgccgtgc agggttacca
      481 ccgggccgcc ctggtaggtg tagagcgcca tgatatcgcc cacgccctcg ccaggaacgt
      541 tgacgccggc ggcctggatc gagtccggtt tcccgaagca gtaattgacg aaatccacgt
      601 cgtgaatgtg cagatccacg agagcgccgc cgccgcgggg gttgtccatc agccagttgt
      661 cccagctcca ggtgggtttg gggctttggc gaacgaaatg ggccgacagc actttgccgt
      721 actcgcccga tttcacgtaa tcgtaggcct tcgcgtaggc aggccagaac cggatgcact
      781 gcagcaccat caggttcttg tcgtacccct ccagcgccgc aaggatttcc ctgccctcgt
      841 ccgaagaacg gacaaacggc ttttcacaca tgacgtgctt gccggccttt atggccttca
      901 gcacatattc cttgtggagg taggttggca gcgtgaccag cgcgatctcg acatccgggt
      961 cggccagcag ctcatcccca tcgctgtacg ttttgacacc gtccaggctg aggtcgcctt
     1021 ccagggcgat attgcctccc tggacggccc ctttacccag gtttgccgga ttcttgtcgg
     1081 cgacggccac gatttcggcc atccccgtgc gccgcaggga gccgaggtgc atcttgccga
     1141 tgaacccgag tccgataatt cccaccttgt gcatgatcct ctccttcatt gcttcatctt
     1201 gtgacgaagt gttacgtgtt cacccatatt tttcgagcag tctgcgcatg tctttcgcaa
     1261 cctggcgcgc cagctcttca tcgggcaggc ccttctcgct gacgagcatc tccggagtga
     1321 tgtagccgtc gtatcccaca ttcttgagcg cgccgaacac ggcctcccag ttcagcgtgc
     1381 ccttcatcag gctgtccgtg aaatccgcca gcgtgccgcc gccgtcatgg gcagtaaaat
     1441 tcttgacatg cacccggccg atgcgtttcc cgcccaaaac ttcgatccag tgctccggat
     1501 atcccgtcag cagcacgttg cctgcatcaa aataggattt cacaaacggc gactggaagg
     1561 aatcgatgta ctgagccagc tcaaacgggc cggtcagaaa cttgttccag acgttctcga
     1621 cgcaaatggc cacacccgcc ttttctgcaa acggcagcag ctccttgatc gaccgctgcg
     1681 agatatccca caccgtcttc gaaggcacca ccgggcgcga tggatcaaac ggcacgttga
     1741 cggcgcccgg caccaccagg atcgcgtcga ccccaagcac gctggcggcg ttgatgtacg
     1801 ctttggtgaa ttcgacggcc tccttccgtt cgggctcgtc cgggctcgac agcgatttcg
     1861 cccagtaaac ccccgtggcc agcgaagaaa tggcgaatcc catcccttca atgccctctc
     1921 tcatggcggt cagagccgcg tcggaggttt ccggactcaa ctcacccgcg ccaaaacata
     1981 gttcgatggc gtcaaagccc gcgtcttgtg ccagcttcgc tgcttcgaga acaggcgtct
     2041 cgttgttgaa cccaccgacg gtccatccgc ttacgctgat tttcatggca agactcccct
     2101 gtagatggtt ccagccttcc ggctgcgtgt ctgcattgct gtgcccccgg cgcgcctgtc
     2161 cgcaggcagt ctgcctctcg atctggcgcc ggggcggctt gcaccgcctc taaagtaccg
     2221 taatcgagcg aggggatcaa tgcgggagcg cgcgaaaacc gcggccgccc atctaaaatc
     2281 cgaacaaaaa ccctcaaacg gccgtatggc ggagcgggcg ggcgcccgtt tcgaatccgt
     2341 tctctccccc gccgtatttc cgcgcaacaa gccgaaccaa cccaagatac cgcccttcat
     2401 cggaacggag ggcgccggaa agtgaaaaaa acgttcagtt ggtggccatt tgtgcggaaa
     2461 taacaaatgt gatcgcccgt cagaccggcc ggcacaccac gatctggcgg cccagacccg
     2521 cctcaaacgg ttctccggaa aatttcccga gtttctccag gatttcaaac cggttgtagc
     2581 ggagtatcat gtccagttcc tgcgggtaga aacaccggag gtggatcgtc tcctcgcgaa
     2641 tctcgtcggt cccccctccc gtgctgtacc aggtgatgtg caagacctgc gccgcatcat
     2701 catagtcagt cgtttcttcg acgaagacgt cggcgccgga ttccttgtcc gtgtaagtaa
     2761 atatacgggc tcgcgcgcct gcgccgctcg aaagcacttc cacgttcggg ttgaacacat
     2821 caaatatgaa gagcccatca gccttcagat gcgtttggac acgttccaga aaggattcga
     2881 gcgagggaag gtcatgcaga tgctgcatgg cgctgaaagg gcagattatc agcgtgaatt
     2941 ggcgcccgag cgcgaaatcc cggcagtcgg cctccacgag cgagatcgga agatcctcgt
     3001 gcgccgcctt gtcgcgaagc cgcgccagca tggccggggc gacatcgagc ccgacgactt
     3061 cgatgccttc gcgcgcgatg ggcagcgtca cgcggccggt tccgcacgcg atctccagca
     3121 cggggccgcc cgcccgcttc gcgcgctcaa cgtagaacgg gatgtccagc acataatcgt
     3181 cgttttcggc gtcgtattgc cgggcgtcca ggtaatattc ttcgatgcgc cccaagtcca
     3241 ttacggtttt ctctgtccct gccagaccgc gcccgccgcc ggccagaaat cacatcggcc
     3301 atgcacgacg ccgggcctcc cgcaacacat gaacggctgg cagtcgttct ctccgcttat
     3361 tgtctgtgtg ctcagccccg ggtcaggttc tgctcaacaa aatcccagtt gatgaggttg
     3421 tcgagtacgg cctcaatata tttgggccgg gcgttgcggt agtcgatgta atacgcatgt
     3481 tcccacacat cgatggtcag cagcgcggtc tggccatgtt tcatgggcag atcggcattc
     3541 gaggtctgcg caattttgag tttgccgtcg tcgagaacca gccacgccca accggagcca
     3601 aacaatttcg ccgcagcccc gctgaattct tccttgaacg cgtcgaaaga cccgaaatcg
     3661 cggtcgatgg cctccgccag ggcgccggaa ggttctccac ctccgccggg tttcaggcag
     3721 ttccagtaaa aggtgtggtt ccagacctgg gccgcattgt tgaatacgcc accctcggct
     3781 ttcatgatga gctcgtcgag cggcatcttt gcttcgggct tgccttcgac aagcgcattg
     3841 aggttattga catacgcctg gtggtgctta ccgtaatgaa attcgagggt ctccgcactg
     3901 atgatcggtt ccagcgcgct cctgctgaac ggaagcgaag gtagtgtaaa cgccatggct
     3961 gcctccttgt acgtcgggct ccgcccgaaa gcctctgcat gcgtccggtt ttcacaaact
     4021 gcaccgcaac ccatcgatac ggtagactgc ggacgcccca gcccagactt gtgccgcccg
     4081 tccacagtag tccactttgc tgaatgaggt caagtggcgt gcacgtgagt gcgccgctgg
     4141 ccctgcgctt cagagcgtcc cggttgacgc caccagggca aagatgccga attgcggcgc
     4201 ttaattccgt tcgggcacca ccgtgtcaag cgttttgcgg atgaggccgc ccagctcccg
     4261 ggacgagaag ggtttcatga gaaactcgca aatacccaat tcccgggcct gctccggcgt
     4321 gatgccttcg ctgtaacccg tcagcaaaaa caccggaaac cccggccggg tctccagtat
     4381 gcgctttgcc aattccacgc ccatcagatg cggcataatc tggtcgacaa tgaccaggtc
     4441 atagctgtcc gggttcgcgc aaaacgcctc aaacgcggcc aggctgttgg tgaacgcgtc
     4501 gacccggtag ccgagctggt gcagcatcat ccccaccatc tg
//
LOCUS       DAOPBQ010000290         4538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15816_length_4538_cov_4.020745, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000290 DAOPBQ010000000
VERSION     DAOPBQ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15816_length_4538_cov_4.020745"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            462..947
                     /locus_tag="PKW60_09750"
     CDS             462..947
                     /locus_tag="PKW60_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99756.1"
                     /translation="MGRSSHQHLTGKFAEGKCEACADHFSCSWRLFYERKPCPVIPWN
                     VWIATGLLAADAVFSVFRLFGAQSLLEGAISSILSLVCIVGLACRAKWAYVLTLLFAG
                     AGLGLGLAGHLGPAAMVINGLLVLLLAPAFRVYFPRPVEPTQDFLDRMPPSSDIPPPV
                     E"
     gene            complement(863..1606)
                     /locus_tag="PKW60_09755"
     CDS             complement(863..1606)
                     /locus_tag="PKW60_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPJ99757.1"
                     /translation="MSNAERNRLRSLVAWIDQKLVQAYGEPRPPRQGDPLGGLIATIL
                     SQNTNDINSGRAYEALISAYPAWDAVMRAPRKQLEQVLRPGGLARTKSARIQHILRQI
                     AETGALSLDSLRDMTNAEAEAYLLGFEGVGYKTARCVLLFDLGRDVFPIDTHVHRILT
                     RMGVIPPGMSADRAHEHVPPFIAKGRCFSLHVNLIRHGRLVCHARNPACMSCPLRRRC
                     VYYSTGGGMSDDGGMRSRKSCVGSTGRGK"
     gene            1851..2384
                     /locus_tag="PKW60_09760"
     CDS             1851..2384
                     /locus_tag="PKW60_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99758.1"
                     /translation="MGCALFMLAALVIIGLLAGLGYYLFTKGLEQTKSQLAAALDEEY
                     GKLIEEDAIPDEHRPLFDELHALGTDPGTSSLAVIMCLAAVQSAFDDGQLTEEEVRHV
                     TEIRDFVKANPQAGMRTIFSFLAEHPEFRQMFEGIEESLGVPLEPGMPEDTLDSSEAA
                     SEAIPGVIREEPAAAAP"
     gene            2441..3706
                     /locus_tag="PKW60_09765"
     CDS             2441..3706
                     /locus_tag="PKW60_09765"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPJ99759.1"
                     /translation="MASVDKLAIDGGPRVAEEAFPVGFLGSAEIGEPEIEAVTSVLRN
                     KTVFRFLDPENSWCARLETKFKGLTGREYALAVGGGTAALLCGLSAVGIGDGDEVIIP
                     GYTYIATAAAILLCGAVPVICEIDETLTMDPADVERKITPRTKAIVPVHMRGLPCDMS
                     RLVSLACKHKLLLVEDVAQACGGSYKGRPLGSFGEAGCFSLQQYKVITSGEGGIVVTD
                     NPDIFQRAALYHDSAMLFWRPGETQVRAFPGTNFRMNELEGALGCTQFERLEGILART
                     RKVKRGIVDQLQNVEGVRLAPVNCPEGDLGIAAILLCETGQQAQRFAAALNAEGIPAS
                     SVYDKTVPDRHIYCYWEYVMDKSSSDPHGRPWTSPLHDQSRRYTPDMCPQTLDILGRS
                     IHIDITQRYEQKHVDWIVEAVKKVAAALA"
     gene            complement(3835..>4538)
                     /locus_tag="PKW60_09770"
     CDS             complement(3835..>4538)
                     /locus_tag="PKW60_09770"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPJ99760.1"
                     /translation="GAGISVESGIPDFRSPTGIWSKYPPEHYATIDAYLANPPKVWRF
                     WRELAIDFAECGPNPAHFALATLENHGYLKAVVTQNIDALHQAAGSKSVVEYHGSARN
                     LFCLDCGAERPMDIRTMSDTPPLCPVCNGLMKPGIVMFGELIPQEALRASAEWGETCD
                     AVLIVGTSAQVYPAADLPYMAKRNGARIIEANIERTSFTETITDVFLQGPAGETLPRL
                     VHTLGLEMPGASAAD"
BASE COUNT          844 a         1382 c         1421 g          891 t
ORIGIN      
        1 gtgctcgaat gccgcgcgga agcccacgaa caggttcatc tcggtctcgc ggcccacagg
       61 ccagactggc cgggctgcgg cgaattccgt ttggccggtg gcattgagta acaggcacaa
      121 tgaatacaac gctgcgctca tcgatcgttc tccatggttt tcgtttcagc cgcgacatcc
      181 agccgctcag acaattcgca tggaagcggg ggtttcacac cagggcgcgc cggttccacc
      241 gaattttgag ggcccggaat gagccctgat cccggcagat ccggaagaag agcccttcaa
      301 accgcctccg tgccccctga gcatttcgca acgactatac cgcctctgta cgcgagaggc
      361 aatcgcgggc ggcttgcgat cgcttgcacg tttttgaaat tatatgtgtg catatacgag
      421 cgtggttgca cgatagagca cccggagcct ggagaccgac gatgggccgg tcatcacatc
      481 aacacctgac agggaaattt gccgagggca aatgcgaggc gtgcgcggat catttctcgt
      541 gctcgtggcg cctgttttat gagcgcaaac cctgccccgt cattccctgg aacgtctgga
      601 tcgccaccgg gctgctggcc gccgatgccg tgttcagcgt attccgcctc ttcggcgccc
      661 agtcgctgct ggaaggcgcg atctccagca tcctgagcct ggtgtgtatc gtgggtctgg
      721 cctgccgcgc aaaatgggcc tacgtcctga cgctgctctt cgcgggagcg ggcctggggc
      781 tcgggctggc gggacatctc ggccccgccg ccatggtcat caacggcctg ctcgtgttgc
      841 tgcttgcccc ggcgtttcgc gtctacttcc cccggccggt cgagccgaca caggattttc
      901 tcgaccgcat gccgccatcg tccgacatcc ccccgcccgt ggagtaatag acgcagcgcc
      961 gccgcaacgg gcacgacatg cacgccggat tccgggcatg gcagaccagc cgcccatgtc
     1021 ggatcagatt cacgtgcagt gaaaagcacc gccccttcgc aatgaacggg ggcacatgct
     1081 cgtgtgcgcg gtccgcgctc atgccgggcg ggatcacgcc catccgggtc aagatgcgat
     1141 gcacatgcgt gtcgatgggg aagacatcgc ggcccaggtc gaacaacagc acgcaccggg
     1201 cggtcttgta gcccacgcct tcaaaaccca acagatacgc ttctgcctcc gcgttcgtca
     1261 tatcccggag tgaatccagc gacagggcgc ccgtttccgc gatctgcctg agaatgtgct
     1321 ggatgcgtgc gctcttggtt cgggcgagtc cgccggggcg cagcacctgt tccagctgct
     1381 tgcgcggcgc gcgcatcacg gcatcccagg cagggtaggc ggagatcagg gcttcgtagg
     1441 cgcggccgct gttgatgtcg ttggtgtttt gcgacaaaat cgtcgcgatc aggccgccga
     1501 gcgggtcgcc ctggcgcggt ggcctgggtt ccccatatgc ctgaacgagc ttctggtcta
     1561 tccaggcaac cagcgagcgc agccggtttc tctccgcatt actcatcgca gttctcccca
     1621 cgcaggttgc gcctgatctg atctgtgctc catgattggc tgcgcggccc ggttaatcca
     1681 cttttgcctc cgccggtttc tccgtataat gggcgcggca ctctccgaga ggacggagcc
     1741 ttgcgccatc gcggcgaagc cagggaggag gggtctttat gtcacagcaa gagctgactg
     1801 cgcaagttct gcggagggat gaaggtccgg gaaagtttgg aacatgttgc atgggctgcg
     1861 cgctttttat gctcgcagct ctggtgatca tcgggctcct ggccggcctc ggatattatc
     1921 tgttcacaaa ggggctggaa cagaccaagt cccagttggc tgcggcattg gatgaagagt
     1981 acggaaaatt aattgaagaa gatgcgatcc cagacgagca ccggccgctt ttcgacgaat
     2041 tgcatgcact gggcacagat ccgggcacgt cgagcctggc tgtaatcatg tgtctggcgg
     2101 cggtacaatc cgcgttcgat gatggtcaat tgaccgaaga agaagtcaga cacgtgactg
     2161 agatccgcga ttttgtcaaa gcaaatcctc aggctggcat gaggaccata ttctcgtttc
     2221 ttgcggagca tcccgaattc aggcagatgt tcgagggcat cgaggagtcg ctgggcgtgc
     2281 cgttggaacc cggcatgcct gaggatacgc tggattccag cgaggccgct tcggaagcga
     2341 ttccgggcgt tatacgggag gaaccggccg cggcagcgcc atgaatgcgc gtccgcgcgg
     2401 gaatggatcc gcggcgtcca tcttttagag gagtcacacc atggcatcag tcgacaaatt
     2461 ggcaattgac ggcgggcccc gcgtggccga ggaagcattt cccgttgggt ttctgggttc
     2521 ggcggaaatc ggagagcccg agatcgaagc tgtgacgtcg gtgcttcgca acaagacggt
     2581 gttccgtttt ctcgaccccg agaattcgtg gtgcgcgcgg cttgagacca agttcaaggg
     2641 cctgaccggc cgggaatatg ccctcgccgt cggaggcggc acggccgcgc ttctctgcgg
     2701 gctttccgcc gtgggcatcg gcgatggcga cgaagtcatc atccccggtt acacctatat
     2761 tgccaccgcc gccgcgatcc tcctgtgcgg cgccgtgccc gtcatctgcg aaatcgatga
     2821 aacgctgact atggaccctg cggatgtcga gcgaaagatc acgccccgca ccaaagccat
     2881 cgttcccgta catatgcgag gccttccctg cgacatgagc cgcctcgtct ctctggcctg
     2941 caaacacaag ctgctcctcg tagaagacgt tgctcaagcc tgcggcggct cgtacaaagg
     3001 ccgtccgcta ggctcgtttg gcgaggcggg ctgtttcagc ctgcagcaat acaaggtgat
     3061 tacctcgggc gaaggcggca tcgtcgtgac cgataacccg gacatattcc agcgcgcagc
     3121 gttataccat gattccgcca tgcttttctg gcggcccgga gaaacgcagg tccgcgcatt
     3181 tcccgggacc aatttccgga tgaatgagct tgaaggcgcg ctggggtgca cccagtttga
     3241 acgcctcgaa ggcatcctgg cccgcacccg caaggtgaag cggggcatcg tcgaccaact
     3301 gcaaaacgtt gaaggagtgc gtctcgctcc tgtcaactgt ccggagggag atctcggcat
     3361 cgccgccatc ctcctctgcg agaccggcca acaagctcaa cgtttcgccg ccgccctgaa
     3421 cgcggaaggc atccccgcca gcagcgtcta tgacaaaacc gtgccagacc gccacatcta
     3481 ctgttactgg gagtatgtca tggacaagtc gtccagcgac ccgcacggac ggccgtggac
     3541 gtcacccctc cacgaccaga gccggcggta tacgcccgac atgtgccccc agaccctcga
     3601 catactcggc cggtccatcc atatcgacat cacccagcgg tatgagcaga agcatgtgga
     3661 ctggatcgtg gaggcggtca agaaggtggc ggcggcgctt gcctgaagcg ggcatctacc
     3721 tcagaactgc ggcgttcgaa tgaacggatc gcatgcacgc ggctgaggtc cagggtgcga
     3781 cacaaggctg gcgctgtacc gtgcagaaag ccggcttaga gtcttctgcg gccttcagtc
     3841 tgcagcactc gcccccggca tctccagccc gagggtatga acgagccggg gcagggtctc
     3901 gccggcggga ccttgcagga agacgtcggt gatggtctcc gtgaagctgg tccgctcgat
     3961 attggcctcg atgatgcgcg cgccgttgcg ttttgccata tagggcaggt ctgcggcggg
     4021 gtagacctgg gctgaggtgc cgacaatgag gacagcgtcg caggtctcgc cccattccgc
     4081 cgaggcgcgc agcgcttcct gcgggatcag ttcaccgaac atgacaatgc cgggtttcat
     4141 gagcccgttg cacaccgggc aaagaggggg cgtgtcgctc atggtccgta tatccatcgg
     4201 acgctcggcg ccgcagtcca gacagaacag gtttcgcgcg ctgccgtggt actcgaccac
     4261 cgatttgctt ccggccgcct gatgcagggc gtcgatgttc tgtgtcacga ctgctttcag
     4321 gtaaccatgg ttttcgaggg ttgccagggc aaaatgcgcg ggattggggc cgcattccgc
     4381 gaaatcgatg gccagttccc gccagaaccg ccagactttg ggcggattcg caagataggc
     4441 gtcgatggtg gcgtagtgtt cgggcggata cttcgaccag atgcccgtgg ggctgcggaa
     4501 atccgggatg ccgctttcga cggagatgcc cgcgccgg
//
LOCUS       DAOPBQ010000291         4526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15860_length_4526_cov_4.202639, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000291 DAOPBQ010000000
VERSION     DAOPBQ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15860_length_4526_cov_4.202639"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PKW60_09775"
     CDS             <1..671
                     /locus_tag="PKW60_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPJ99761.1"
                     /translation="GPAPAIPASQLTFAVTTLVQRMYPSNEEPAADGVRDASALDPLL
                     RGTLVHRYFELWDFQHAPPDIGAFLRREKPSYHLADRLRDTLESAVQRFMASEAWPLV
                     TGAREILREAPFMLRVGDAVLEGVIDVVLDGNAVLDYKTGLHRESRRLMYEAQLCLYA
                     EAMRRLLGRLPERAWIYYVDSGELVEADISPDRTAEVLALAGETMKMLRNEPEPDEME
                     AYLQ"
     gene            668..1630
                     /locus_tag="PKW60_09780"
     CDS             668..1630
                     /locus_tag="PKW60_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99762.1"
                     /translation="MRRLVLALCLASLTFSAWGAVAQTLHDGLFYVEYPEGEHQTAVV
                     TLENLQRIAAAWRHRLDPGEAPILVRICASQGDFAANAGFWPPAEVGGVAHSEEGRIV
                     LRTPSQLPNPGLYDGMVRHELIHVLLARNTSLEHLPRWLNEGTAMHISGEHRWNTSFH
                     VARMYLSGRLYTYDDLMLDFSMVKGERPFGDLYAQSLSMTAFLYDRLGEETFWSLLYS
                     LRDKDFSGALGEIAGMSPHEFWTAWYRSLWKVAVISAVMSGFGIFDFMALLVIVGYFR
                     RRHHNRKVLARWELEEAEEPPMMAAWELEGGSEYPWEMDDEEDP"
     gene            1682..3730
                     /locus_tag="PKW60_09785"
     CDS             1682..3730
                     /locus_tag="PKW60_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase"
                     /protein_id="HPJ99763.1"
                     /translation="MGSRLNVGALKAGPGHGIPLGASAGETGVHFALVSRHARRVWLA
                     LFENPDDARPAVEFELDPERNRIGDVWSIFIENLHAGAYYLFRLDGPCEPPKGHLFNP
                     ECYVLDPYALMVTGSVQDLEAKCVVVDIERGWEPYLRSRTRKANSIVYEVHVKGFTAH
                     ESSGVGHRGTYRGFIEKIPYLQELGVTAVELMPVQEVGEEYLKRRNPKTGERLKNYWG
                     YAPIAFFAPAARFGSEGGIGEQLLEFRELVAALHQAGLEVILDVVFNHTAEGDEKGPT
                     LCFRGIDHSIYYLLDADGKCLNFTGCGNTVNCNHPFVRDLIRDCLRYWTTVMRVDGFR
                     FDLASVLGRDQSGKLVENAPIIERIAEDPILRDVKLTAEAWDVGGAYQVGSFGNNGRW
                     SELNGKFRDDVRRYWINDPDAKGAFALRITGSPDLYQDDGRRPIHSVNFVTSHDGFTL
                     NDLVSYNKKHNEGNGEKNRDGMDHNHSWNCGVEGETNDPVILALRLQMQRNFLATLFI
                     SLGVPMLLGGDEFGRTQKGNNNAYCQDNEISWHDWGLLEKNAALFRFCKGMIQFRKEN
                     AVFERATFYTGNPSRKDAKPDVRWFRPDGQAQDWQPVQPALGFWINGTENDGVPLYLM
                     FNPTPDDSEFVLPGGDWWLRVDTAAASPGDLYAADKAPRAGETGRMTVAARSMVILSG
                     "
     gene            complement(3789..>4526)
                     /locus_tag="PKW60_09790"
     CDS             complement(3789..>4526)
                     /locus_tag="PKW60_09790"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPJ99764.1"
                     /translation="EVLGAVPSPDGAIPDDEDIEHALRTVGPAAHHAHRLVEALEQDG
                     ARGTQSEEERRWLGVCAAIGEQTGMPPTQVRLGALALGLLTGPFALVIYIGLFFVQRA
                     SGRSAVSPIEKWVLTKYVLGLILGAVALRYVAWGFVALLNYGYGLLFKEALVLSGLWN
                     WLQRYDGALFFWTLFILTPLAVLAGLPVPTPWRNTLRKVFEAGLALYAAALCFGAGCA
                     VAGTILNAAQRMQTSPIIDFQAMFSAM"
BASE COUNT          872 a         1330 c         1445 g          879 t
ORIGIN      
        1 tgggccctgc gccggccatt cccgcaagcc agctcacttt tgccgttacc acgctcgtgc
       61 agcgcatgta tccgtccaac gaagaaccag cggcagacgg cgtccgcgat gcgtccgcgc
      121 ttgatcctct gcttcgcggg actttggtgc accgctattt cgagctttgg gatttccagc
      181 acgcgccccc ggacattggc gcttttctcc ggagagagaa gccgtcttat catctggcgg
      241 accgcttgcg cgacacgctg gagagcgcag ttcaacgctt tatggcgtcc gaagcgtggc
      301 ctctggtgac cggagcgcgc gagattctgc gtgaagcgcc gtttatgctg cgcgtgggcg
      361 atgccgtgct cgaaggcgtg atcgacgtcg tacttgacgg gaatgcggtc ctcgattaca
      421 agaccggcct ccaccgcgag agccgccgct tgatgtatga agctcagttg tgcctgtatg
      481 ccgaggccat gcgccgcctg ctcggccggc tgcccgaacg ggcctggatc tactacgttg
      541 actcgggcga gctggtcgag gccgacattt ctccggaccg gaccgccgaa gtgcttgcgc
      601 ttgccgggga gaccatgaaa atgctgcgga atgagcccga gcccgacgaa atggaggcct
      661 atctgcagtg agaaggcttg tcctggcgct gtgccttgcg agcctgacgt tttcagcgtg
      721 gggggcagtc gctcagacgc ttcatgacgg tctcttctat gtggaatatc cggaaggcga
      781 gcaccagacg gccgtggtca ccctggaaaa tctccagagg attgccgcgg cctggcgcca
      841 ccggcttgat cccggggaag ctcccattct ggtgcgcatc tgcgcgtctc agggcgattt
      901 tgccgcaaac gccgggtttt ggccgccggc ggaagtgggg ggcgtcgccc attcggaaga
      961 ggggcgtatc gtgctgcgca cgccgtcgca attgccgaat ccaggcctgt atgacggcat
     1021 ggtgcggcac gaactgattc atgtgctgct ggcgcgcaac accagtctgg agcatctgcc
     1081 ccggtggttg aacgaaggca cggccatgca catctcaggg gagcaccgct ggaacacctc
     1141 gttccatgtt gcgcggatgt atctgtcggg ccggctctat acctacgatg acctgatgct
     1201 cgatttcagc atggtgaaag gcgagcggcc gttcggtgac ctctacgccc agagcctttc
     1261 gatgaccgct tttctgtacg atcggctcgg cgaggagacc ttctggagcc tgctgtattc
     1321 gcttcgcgac aaagactttt cgggtgcgtt gggcgagatc gccggcatga gcccgcacga
     1381 attctggaca gcctggtacc gctcgctctg gaaagttgcc gtcatctccg ccgttatgtc
     1441 aggattcggc atattcgatt tcatggcact gctggtgatc gtggggtatt ttcgaaggcg
     1501 tcatcataac cgcaaggttt tggctcggtg ggaactggaa gaagccgaag aaccaccgat
     1561 gatggcggcg tgggaactcg aaggcggcag cgaatatccc tgggaaatgg atgacgagga
     1621 agacccatga ttttgcgctg cgccgcggcg caggcgcgga aaaatccgtc aggaaggtgg
     1681 tatgggcagt aggctgaatg ttggggcgtt gaaggcgggt cccgggcacg ggatccctct
     1741 gggggcttca gcgggcgaga ccggggtgca ctttgccctg gtgagccgtc atgccaggcg
     1801 cgtgtggctg gctctgttcg agaacccgga cgacgcgcgc cccgccgtgg aattcgagct
     1861 ggacccagag cgcaaccgca tcggcgacgt gtggagcatc tttatcgaaa acctgcacgc
     1921 gggcgcgtat taccttttcc gcctggacgg gccctgtgag cctccaaaag gccatctttt
     1981 caacccggag tgctatgtgc tcgaccccta cgcgctgatg gtgacgggaa gcgtgcagga
     2041 tctcgaggcc aagtgcgtgg tcgtcgacat cgagcgcggc tgggagccgt atctccgatc
     2101 gcgaacgcgc aaagcgaact cgattgtata cgaggtgcac gtcaaagggt tcaccgcgca
     2161 cgaatcctcg ggcgtggggc atcggggcac ctatcgcgga tttattgaga aaataccgta
     2221 tctccaggag ctgggcgtca ccgcggtcga gctgatgccg gtgcaggaag tcggcgagga
     2281 atatctcaaa cgccggaacc cgaagaccgg agaacgcctt aagaactact ggggttacgc
     2341 gcccatcgcg ttctttgccc ccgccgcgcg attcggaagc gaaggtggta ttggcgagca
     2401 gttgctcgaa ttccgtgagc tggttgcggc tctgcaccag gccgggctcg aggtcattct
     2461 tgacgttgtg ttcaaccaca ccgccgaagg tgatgaaaag ggccccacgc tctgtttccg
     2521 cggcatcgat cactcaatct attatctact tgatgcggac ggaaaatgcc tgaacttcac
     2581 gggctgcggc aatacggtca actgcaacca tccgtttgtg cgcgatttga ttcgcgactg
     2641 cctgcggtac tggaccaccg ttatgagagt cgacggattc cgtttcgatc tggcgtcggt
     2701 cctcggccgc gatcaaagcg ggaagctcgt ggaaaacgcc ccgatcatcg agcgcatcgc
     2761 agaggatccc atcctgcgcg atgtgaaact caccgcggag gcctgggacg tgggcggcgc
     2821 gtaccaggtg ggatcgttcg gcaacaacgg gcgctggtct gagctcaacg gcaagtttcg
     2881 tgacgatgtc cgccggtatt ggatcaatga cccggatgcg aagggcgcct ttgcgttgcg
     2941 catcaccggc agccccgatc tgtaccagga tgatggccgc cggcccattc acagcgtcaa
     3001 tttcgtcact tcccacgacg gattcaccct gaacgatctc gtctcctaca acaagaagca
     3061 taacgagggc aacggagaaa agaaccggga cgggatggac cataatcaca gctggaactg
     3121 cggggttgaa ggcgaaacca atgatcccgt cattctggcg ctgcgtttgc agatgcagcg
     3181 gaactttctc gcgactcttt tcatctcact cggtgtgccc atgctgctcg ggggcgacga
     3241 attcggccgt actcagaaag gcaacaacaa cgcctattgt caggacaacg agatctcatg
     3301 gcacgactgg gggctgctgg agaagaacgc cgccctcttc cggttttgca agggcatgat
     3361 tcagtttcgc aaggaaaacg ccgtgttcga gcgtgcgacg ttctacaccg gcaacccgtc
     3421 gcggaaggac gctaaacccg atgtgcgctg gttcaggccg gatggccagg cgcaggattg
     3481 gcaacccgtt caaccggcgc tgggattctg gattaacggt accgaaaacg atggcgttcc
     3541 tctctattta atgttcaacc ccacgccgga tgacagcgag ttcgtccttc ctggcggcga
     3601 ctggtggctg cgcgtggata ccgccgctgc gtcacccggt gacctgtatg cggcggataa
     3661 ggcgccgcgt gcgggcgaga cgggcagaat gaccgtggcc gcccgttcga tggtcatcct
     3721 ctccggctga tacactccgg gcgtacgtac tgcaggcgtg gcgtgcgccc gtccggttct
     3781 gggcttgctc acatggccga gaacatggcc tgaaaatcga tgatggggga ggtttgcatg
     3841 cgctgcgcgg cattcaggat ggttccggcc accgcgcaac cggccccgaa gcacagggca
     3901 gcggcataaa gggcgagacc ggcttcaaaa actttgcgca aggtattgcg ccagggggtg
     3961 gggaccggga gccccgccaa cacggccagc ggcgtcagga tgaacaaggt ccagaagaac
     4021 agcgcgccat cgtagcgctg aagccagttc cacaggccgg agagtaccag cgcttctttg
     4081 aacaacaggc cgtaaccgta attcaggagc gccacaaacc cccacgccac gtagcgcagc
     4141 gccaccgcgc ccagtatcag gcccagcaca tacttggtca gaacccattt ttcgatgggg
     4201 gacacagcac tccgtccgga cgcgcgctgt acaaagaata atccgatgta aatgacgagc
     4261 gcgaatggcc cggtgagcag gcccagtgcc aaggcgccca ggcgcacttg cgtgggaggc
     4321 atgccggtct gctcgccaat ggccgcgcag acgcccagcc agcggcgttc ttcctcggat
     4381 tgggtcccgc gcgccccgtc ttgttcgagt gcttccacca ggcggtgggc atggtgcgcg
     4441 gcagggccta cggtacgcag cgcatgctcg atgtcctcgt cgtccggtat agcgccgtcc
     4501 ggcgaaggta ccgcccccag aacctc
//
LOCUS       DAOPBQ010000292         4513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15912_length_4513_cov_3.966353, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000292 DAOPBQ010000000
VERSION     DAOPBQ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15912_length_4513_cov_3.966353"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..915
                     /gene="hprK"
                     /locus_tag="PKW60_09795"
     CDS             70..915
                     /gene="hprK"
                     /locus_tag="PKW60_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPJ99765.1"
                     /translation="MFISDINRPGLALGGYLEYFANDRIQILGNTEIHYMEKLSTSEL
                     QHRLEAMFAFEIPAFVLSRSLRPRNIFLDMCNRRGIPVLRSRRSTDEVISSIILFLAE
                     EFSPETVIHGTAIDCYGVGILIAGAPGIGKSETALELVERGHRMVADDVVTVRRGREG
                     IVFALASPVIEHHMEIRGVGIIDVKSVYGVGRVRNSKRISLVVELEMWNETAHYDRTG
                     LVDEYVDILRTRIPYLLIPVRPGRNIAIIVEVAALNHRLKELGVHPAQQLNQRLLSLM
                     SAGDA"
     gene            908..1777
                     /gene="rapZ"
                     /locus_tag="PKW60_09800"
     CDS             908..1777
                     /gene="rapZ"
                     /locus_tag="PKW60_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPJ99766.1"
                     /translation="MPDSHRFVLVTGLSGAGKSLALRFLEDLGYYCVDNLPAALLPTF
                     AELIQGSKEPARRKVAVCLDARAGQGLAELPGYVQEVEEKGTRVEALFLDSSDAVLIH
                     RYSETRRRHPCSPAGSVEEGIQRERELMQPMRSRADLIIDTSSTSVAELRERIASMFL
                     GPKEMRELAITVLSFGFKRGVPPEADLVFDVRFLPNPYYDLDLRSLTGAEPDVRDFVL
                     DNADGREFLDRIKGLLKFLIPRYAAEPKAYLTIGVGCTGGQHRSVAVAHAISVMLREM
                     NYNVRLRHRELPG"
     gene            2093..2365
                     /locus_tag="PKW60_09805"
     CDS             2093..2365
                     /locus_tag="PKW60_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPJ99767.1"
                     /translation="MPELTEVMAVVNSMGLHARPAAKLVQTVLQFESDVQINVNGQRV
                     NAKSIMGLLTLAAAQGTRMTVTVQGADAEQAMQAVRELIASGFGES"
     gene            2370..4136
                     /gene="ptsP"
                     /locus_tag="PKW60_09810"
     CDS             2370..4136
                     /gene="ptsP"
                     /locus_tag="PKW60_09810"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPJ99768.1"
                     /translation="MEIALRGIGVSPGIAIGPVLPFGLTGLEVPKYQIEDKTSELSRF
                     REAVEGVRIDLKRLLLKTSQDLGPKHAEIFDAHLMVLDDVTFHEDIENRLQGEGWNVE
                     YLLEEFIRRYSSMLESVDDAQFRERTQDVVDVLRRIQSRLLKTEMESLEHLDHPCVVV
                     AHDLSPSDTAKMDLENTLGLVTDLSGPTSHTAILARAFEVPAVVGLGTVGAQALAGDL
                     VIVDGTRGDVILRPTEATLAHYVTEKTRLEEERQALLLADRNLRSITIDGVEIPTLAN
                     IELPVEITHSKTTKAQGIGLYRTEYLFMNRASLPSEEEQFEAYAAVAEAMHPSPVTLR
                     TLDLGGDKFVSHLHLLDEINPQLGWRAIRFCLERPDIFKAQIRAMLRASVHGNVQIMF
                     PLVSGLDEFRRVKKMVREVCADLERRGVPFDSRAPIGTMIEVPSAVVIADLLAAESDF
                     FSIGTNDLIQYGLAVDRANEKIAHMYEPAHPAILRLIRQVVQAAKSARIPCGLCGEMA
                     GDPVFTEILVGLGVDSLSMSAVSIPVVRAEIANIRMNVAKRFARRVLKMGSESAIKKL
                     LYKRYKSRHTLSRVLGRENSHE"
     gene            4197..>4513
                     /locus_tag="PKW60_09815"
     CDS             4197..>4513
                     /locus_tag="PKW60_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPJ99769.1"
                     /translation="MAIRISILGSTGSIGRSALEVVRRYPGMFDVVALGAFANTDLLT
                     RQIAEFRPAHVAVVDEAAAERLQSTGLSARLWRGPQGLEDLAALETDVALCAVVGAAG
                     LKPV"
BASE COUNT          902 a         1336 c         1381 g          894 t
ORIGIN      
        1 cattctgatg ccgcttgatc caatcgcagg agaccagatt catgatgacg gaagggcgtc
       61 ggacggcccg tgttcatctc ggatatcaac cgcccgggac tggcattggg cggatacctc
      121 gaatacttcg ccaatgaccg catccaaatc ctgggcaata ccgaaatcca ttacatggag
      181 aagctctcga cttcggagct gcagcaccgg ctcgaagcga tgtttgcctt tgagatccct
      241 gcgtttgtgc tctcccggag cctccggcca cgcaatattt tcctcgacat gtgcaatcgc
      301 cggggcattc ccgtcctgcg atcgcggcgc agcacggacg aagtgatcag cagcatcatt
      361 ctgtttctcg ccgaggaatt ctcccccgag accgtcatcc acgggacagc catcgactgc
      421 tacggtgtgg gcatcctgat cgcaggggcc ccgggaatcg gcaaaagcga gacggcgctc
      481 gaactcgtgg agcgcgggca ccgcatggtg gcagacgacg tggtcacggt tcgccgaggc
      541 cgtgaaggca ttgtatttgc cctggcatcc cccgtcatcg agcaccacat ggaaattcgc
      601 ggggtcggga tcatcgacgt caaaagcgtg tacggcgtcg ggcgcgtccg taactcaaaa
      661 cgcatcagcc tggtcgttga gctggagatg tggaacgaga ccgcccacta cgaccggacc
      721 ggcctggtcg atgaatacgt ggatatattg cgcacccgga tcccgtacct tctcatcccc
      781 gtccgacccg gccgcaatat cgcgatcatc gtcgaggtcg ccgcgctgaa ccaccgcttg
      841 aaagaactcg gggtgcatcc cgcacagcag ctgaaccaga ggctcctcag cctcatgagc
      901 gcaggcgatg cctgacagcc accgctttgt gctggtcacg ggtctgtccg gggcggggaa
      961 gtcgctggcg ctgcgcttcc ttgaagatct gggctactac tgcgtcgaca acctgcctgc
     1021 ggcgcttctc cccacgtttg ccgaattgat ccagggcagc aaggaaccgg cccgccggaa
     1081 agtggcggtc tgccttgatg cccgtgcggg acaaggcctg gccgaactgc cggggtatgt
     1141 gcaggaagtt gaggaaaagg gcacacgggt agaggccctt tttcttgaca gttcggatgc
     1201 cgtactcatc catcgataca gcgagacacg ccggaggcac ccttgctccc ccgccggcag
     1261 tgtggaagaa ggcattcagc gtgagcgcga gctgatgcag cccatgcgct cgagggccga
     1321 cctcatcatc gatacaagtt ccacctccgt ggcggaactg cgcgaacgta tcgccagcat
     1381 gtttctgggc cccaaggaaa tgagggaact tgccataacc gtcctctctt tcggcttcaa
     1441 gcgcggcgtg ccgccggaag ccgacctggt gttcgatgtg cgcttcctgc caaaccccta
     1501 ttatgatctc gacctgcgct cgctcacggg cgccgagccg gacgtgcgcg attttgtgct
     1561 cgataatgcc gatgggcggg agtttctcga tcggataaaa ggcctgctga aattcctgat
     1621 cccgcggtat gcggccgaac ctaaggcgta tttgaccatc ggtgtaggtt gtacgggtgg
     1681 ccagcatcgc tccgtggcgg ttgctcatgc aatctccgtg atgctgcgcg agatgaatta
     1741 caatgtccgc ctgcgccatc gcgaactgcc cgggtaatct ccgcaaatag acagggatcg
     1801 ccgactgacg cctgtttctg caggctgaga gcaagacacg caccgggccg cggacgttcc
     1861 ggccgccccc gcgctcccgg ccggcggctg cgcctaactt ttttgataat ttgtgcggaa
     1921 tctactatat tttaaccttg cccggcacgt ctgttttgaa cgtgcggagc gaaccaggtt
     1981 tgcccgtatg ccggtttggg atccaggcgg gcagagccac cggagctcag ccagactccg
     2041 tcagtgctat cattattggt atggaaaccg acactatcag gatgttcaaa ggatgccgga
     2101 actgaccgaa gtgatggccg tcgtgaattc gatgggcctc cacgcccgtc ctgcagcaaa
     2161 gcttgtgcag accgtcctgc aattcgagag cgacgtgcag atcaatgtga acggccagcg
     2221 agtcaatgca aagagcatta tggggctgtt gacgctcgcg gcggcacaag gcacccggat
     2281 gaccgtgacg gtgcagggcg ccgatgcgga acaggccatg caggcggtgc gtgaattgat
     2341 agcgtccggg ttcggggaaa gctgatcacg tggagatcgc gctgcgcgga ataggggtct
     2401 cgccagggat agcgataggc cccgtactgc ctttcgggct cactgggctc gaagttccga
     2461 agtatcagat tgaagacaaa acgtcggagc tcagccgctt ccgcgaggcc gtcgaagggg
     2521 tgcgcatcga tcttaaacgt cttttgctga agacgtcgca ggatttgggc cccaaacacg
     2581 ccgagatctt cgatgcccac ctgatggtgc tcgacgatgt caccttccac gaagatatcg
     2641 agaatcgcct gcaaggtgaa ggctggaatg tcgaatacct gctcgaagaa ttcatccggc
     2701 ggtattcgag tatgctggaa tcggtcgacg acgctcaatt ccgcgagcgt acgcaggatg
     2761 ttgtcgatgt gttgcgccgc atccagagcc gcctgctcaa gaccgagatg gaaagcctgg
     2821 aacacctgga ccatccctgc gtcgtggtgg cgcacgatct ctcgccatcg gacacggcca
     2881 aaatggatct cgagaatacg ctgggcttgg tcacggatct cagtgggccc acatcgcata
     2941 cggccattct ggctcgcgcg tttgaagttc cggcagtggt cggcctgggc actgtcggcg
     3001 cccaggcatt ggccggagac cttgtcattg tggatggcac ccgaggcgat gtgattctcc
     3061 ggcctaccga agccactctg gcacactacg tcaccgagaa aacccgtctc gaagaagaac
     3121 gccaggcgct gctgctggcc gaccggaacc tgcgaagcat cacgattgac ggcgtggaga
     3181 tcccaaccct cgccaacatc gagttgcctg tagagatcac gcacagcaag accacgaaag
     3241 cccaggggat cgggctgtac cgtactgaat acctgtttat gaaccgcgcg tcgctgccct
     3301 cagaggaaga acaattcgag gcgtacgcgg cagtggccga agccatgcac ccttcgccgg
     3361 tcaccttgcg aaccctcgat ctcggaggcg acaagtttgt ctcgcatctt catctgttgg
     3421 atgagatcaa cccgcaactg ggctggcgtg ctatccggtt ttgcctcgag cggccggaca
     3481 ttttcaaagc ccagatccgc gcgatgctgc gcgcgagtgt gcacgggaac gtgcagatca
     3541 tgttccctct ggtgagtggt cttgacgagt tccgccgcgt caagaaaatg gttcgtgaag
     3601 tttgcgccga cctcgagcgc cggggggttc cctttgattc gcgagcgccg ataggaacca
     3661 tgattgaagt gccgtccgcc gttgtgatcg ctgacctcct ggcggcagaa tccgacttct
     3721 tcagcatcgg caccaatgac ctgatccagt acggtctggc cgtggaccgc gccaacgaga
     3781 agatcgcgca catgtatgag ccggctcacc cggcgatcct tcgcctgatc cggcaggttg
     3841 ttcaggccgc taaatcagcg cgcattccct gcgggctctg cggggagatg gccggcgatc
     3901 cggtgttcac ggagatcctg gtcgggctgg gtgtggattc gctgagcatg tccgccgtat
     3961 ccattccagt ggtacgcgcg gaaattgcca atatacgcat gaacgtggcg aagcgctttg
     4021 cgcgccgcgt attaaagatg gggtccgaat cggccatcaa aaagctcctc tacaaacggt
     4081 acaaaagccg ccatacactg tcacgggtgc ttggccgcga aaacagccat gaataaccgg
     4141 ggatggagcc cggcctcgac aggccgccgc cgcttcgtgc aaacggaaat ctgattgtgg
     4201 caatacgtat atccatcctg ggttccaccg ggtccattgg ccgaagcgct ctcgaggtgg
     4261 tgcgccgcta ccccggtatg ttcgacgtgg tggcgctggg cgcattcgcg aacacggatc
     4321 tgctcacccg ccagatcgcc gaattccgcc cggcacacgt ggccgtggta gacgaagccg
     4381 cagcggaacg cctgcaaagc accggcctga gtgcccgttt gtggcggggg ccgcaaggtc
     4441 tggaggatct ggcggccctg gagaccgatg tcgcgctttg tgcggtggtt ggtgcggctg
     4501 ggttgaaacc ggt
//
LOCUS       DAOPBQ010000293         4505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15943_length_4505_cov_4.339551, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000293 DAOPBQ010000000
VERSION     DAOPBQ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15943_length_4505_cov_4.339551"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PKW60_09820"
     CDS             complement(<1..502)
                     /locus_tag="PKW60_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPJ99770.1"
                     /translation="MRRLSILTIVSVSMCLAVSADTTPDAPAAAPETFTLWQLPNQTH
                     VQMMSYVIQTPDGSLLVIDGGNAGDADYLREFILARGGQVNAWIITHVHSDHVDAIIE
                     ILKEPGTITVGAFYGSIPEREWVATHAGQSELDTWDALSGALAQSGHQVAELAIGQRL
                     TLDTVNI"
     gene            683..1465
                     /locus_tag="PKW60_09825"
     CDS             683..1465
                     /locus_tag="PKW60_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPJ99771.1"
                     /translation="MNAIVIALAALSGVSASPAAELPAPIPLLCGNITDHFYTFIRDR
                     GRDNDPKQVFTVQDGILRISGEEWGCITTKKEYDNYRLITEFKWGERAFAPREERARD
                     CGIMVHSVGEDGAYGNVWMRSIECQIIEGGTGDFIVVGDGSEDFAITCAAAPEKVAGC
                     HVYQPGGELATIHDGRVNWWGRDPEWKDVKGFRGARDVEKPVGEWNRLECIVKDDEIT
                     VILNGVEVNHAQNVKPSRGKIQVQSEGAEIFFRKIELIPLKP"
     gene            1530..3182
                     /locus_tag="PKW60_09830"
     CDS             1530..3182
                     /locus_tag="PKW60_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPJ99772.1"
                     /translation="MSRSRSGWQRVAALMACLAAIAGLAGCGARETRAARATAEGVLL
                     VGNGAEPQELDPHLVSGVPEHRILSALFEGLVDLDPATLEPIPGVAEWWSVTPDGLMY
                     TFYLRKSAHWSNGDPLTAHDFLYAWERILTPTLASEYASMLHCIVNARAFNRGEIRDF
                     SQVGAKAIDEYTLEVRLEQPTPYFLSLHVHYTWYPVPHKLIESLGGMTARNTKWTQPG
                     HMVSNGAFKLVKWEPDKVIRVEKNPRYWNADAVRLQGIAFYPISDPMTEDRTFRTGGL
                     HLTENVPLPKIEIYQQEQFPFLHIDPYLGTYYYRLNTTRPPFDDVRVRRAFALSLDRD
                     TLVAQVVDAGRQPAAHYVPPETGGYTSTDRMPYDVDQARRLLAEAGYPGGKGFPKVEL
                     LYNTSEDHRRIAEAVQQMWNAALHVEVQLLNQDWKVYLNSMNVLNYDIARSGWIGDYS
                     DPYNFLECFLSGNGNNRTGYASEAYDRLVRKGASVMYKAGRLATYQQAEETLLADAPI
                     IPVYFYTRPYLKSPDVKNWQPNHLGYISWKTLYLEAPEEESP"
     gene            3259..4176
                     /locus_tag="PKW60_09835"
     CDS             3259..4176
                     /locus_tag="PKW60_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPJ99773.1"
                     /translation="MVKYIIRRLLGFVPTLFIIVTVVFFLIRLAPGGPFDAERNVSSE
                     VLQNLEAYYHLDAPLHEQYFQYLRDVLHGDLGPSFEKPSRTVSEWIAYRSPVSLELGL
                     YGLAVALILGLSAGVIASLRPNTLTDHVPMAFAMAGICVPNFVLGPLLVLVLSLHLGW
                     LPVSGWEYSEDKILPAITLGVPYAAYIARLSRGGMLEVLSQDFIRTARAKGLSEARVL
                     LRHALRGGIQPVVAFLGPATAGLITGSFVVENIFQVPGLGSEFVGAALHRDYTMILGT
                     VLFFAALILLFNLLVDVVLAWLDPRLRYE"
     gene            4169..>4505
                     /locus_tag="PKW60_09840"
     CDS             4169..>4505
                     /locus_tag="PKW60_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPJ99774.1"
                     /translation="MSDSRRLENFVPPVAVEQGASLWRDALVRLRKNRLATAGLVVLC
                     ALVLVSLLTPWIAPYGYEEQDLALGAVPPNRAHWFGTDLLGRDLFTRVLYGGRISLLV
                     GFAATGVSLC"
BASE COUNT          832 a         1246 c         1508 g          919 t
ORIGIN      
        1 ggatattcac ggtgtcgagg gtcaaacgct gcccgattgc cagctcggcg acctggtggc
       61 cggattgggc gagcgcgccc gacagggcat cccaggtgtc gagttccgac tgcccggcgt
      121 gggtggcgac ccattcgcgt tcgggaatgg aaccgtaaaa tgcgcccacc gtgattgtgc
      181 cgggttcctt caggatttcg atgatggcgt caacatggtc cgaatgcacg tgggtaatga
      241 tccaggcgtt cacctggccg ccgcgggcga ggatgaactc ccgcaagtag tcggcgtcgc
      301 ccgcattgcc gccatcgatg acgagtagag acccgtccgg cgtctggatg acgtaggaca
      361 tcatctgtac gtgcgtctgg ttcggcaact gccacagcgt gaaggtttcg ggtgctgccg
      421 caggcgcgtc cggggtggta tcggctgaaa cggcaaggca catggagact gatacaatcg
      481 tcaggattga caagcggcgc actctgaatt cctcccgggt tgcaggttcg aggcaattcc
      541 tcctatccat cttcgggaag aatacccgct cagcctgcgg catgacaaag gcgtgcgggc
      601 tgacgacggc tctaccagtc ggggaaggct ttatgtcaca atgaatacag tgaatcctga
      661 ccatcacctg aggtatgaaa ccatgaacgc tatcgttatc gcccttgctg ccctgagtgg
      721 cgtcagcgcc tcgcccgcag cagaactccc ggcgccgatt ccgctgttgt gcgggaacat
      781 cacggatcat ttttacacgt ttattcgtga ccggggccgc gacaacgacc cgaagcaggt
      841 gtttacagtc caggatggga ttctgcgtat ttcgggcgag gaatggggct gtattacgac
      901 aaagaaggaa tacgacaact accgcctgat caccgagttc aaatggggcg agcgcgcgtt
      961 tgcgccacgc gaggagcgtg cgcgcgattg cgggatcatg gtgcattcag tcggtgaaga
     1021 cggtgcctac gggaatgtgt ggatgcggtc gatcgaatgc cagattatcg aaggcggcac
     1081 cggcgatttc attgtcgtgg gggacggcag cgaagacttc gcgatcacct gtgccgctgc
     1141 acccgaaaaa gtggccggtt gtcatgtata ccagccgggt ggcgagctgg cgaccatcca
     1201 tgacgggcgc gtgaactggt ggggccgcga tcccgagtgg aaagatgtca aagggtttcg
     1261 aggggcacgg gacgtcgaga aaccggtggg cgagtggaac cgcctggaat gcattgtgaa
     1321 agatgacgag atcacggtga tcctgaacgg cgtggaagta aatcatgcgc agaacgttaa
     1381 gccgtctcgc gggaagatcc aggtgcaatc cgagggcgcg gagatcttct tccggaagat
     1441 cgaattgatc ccgctgaaac cgtagagatt ccccggccat gactggggca gatgccgccg
     1501 gaagaaaacg attcggagga gaggtttcca tgagccgttc caggtcaggg tggcaacgcg
     1561 ttgcggcgct gatggcgtgt cttgcggcaa tcgccggtct cgccggttgc ggagcccggg
     1621 agacccgtgc cgccagggcg acggccgaag gcgtgctgct ggtcggtaac ggcgcggagc
     1681 cgcaggaact cgatcctcac ctggtgtcgg gtgtccctga acaccgcata ttgtcggcgc
     1741 tgttcgaagg actggttgac ctcgatccgg cgacgctgga gccgatcccg ggcgtggcgg
     1801 agtggtggtc ggtaacgcct gatggcctga tgtacacctt ttatcttcgt aaaagcgcgc
     1861 actggtcgaa cggagatccg ctgaccgcgc acgattttct gtatgcgtgg gagcgcatat
     1921 tgacgcccac gctggcgagc gaatatgcat cgatgttgca ctgcatagtg aatgcgcgcg
     1981 ctttcaacag gggtgagatc cgggattttt cgcaggtggg tgcgaaggcg attgacgaat
     2041 acaccctcga ggtgcgtctg gaacagccca cgccgtattt tctgtcgctg cacgtgcatt
     2101 acacctggta tcccgttccg cacaaactga ttgagtcgct cggcggcatg acagcccgga
     2161 acaccaaatg gacgcaaccc gggcacatgg tatccaacgg ggcgttcaag ctggtgaaat
     2221 gggagcccga caaggtgatc cgcgtggaga agaatccgcg ttactggaac gcagacgcgg
     2281 tgcgcctgca gggcatcgcc ttttatccaa tcagtgatcc gatgaccgag gatcgcacgt
     2341 ttcgcacggg agggctgcat cttacggaaa acgtgccgct gcccaagatc gaaatctatc
     2401 agcaggaaca gtttcccttt cttcacattg acccgtacct cgggacgtac tactaccgcc
     2461 tgaacacgac ccggccgccg tttgacgatg tgcgggtgcg gcgggcgttt gcgttgagcc
     2521 tggaccggga caccctggtt gcgcaggtgg tggacgccgg ccgccagccc gcggcgcatt
     2581 acgtgccgcc ggagaccggg ggatacacga gcaccgaccg catgccgtat gacgtggacc
     2641 aggcgcggcg gctcctggcc gaggccggct atcccggcgg caaaggcttc ccgaaggtgg
     2701 agctgcttta caacacgagt gaggatcaca ggcgcattgc ggaagcggtg caacagatgt
     2761 ggaatgcggc gctgcatgtt gaggtgcagt tgctgaatca ggactggaag gtctatctca
     2821 attccatgaa tgtgctgaat tacgacatcg cgcggtcggg atggatcggc gattacagcg
     2881 atccgtacaa ttttctggag tgtttcctga gcggcaacgg caataaccgg accggctatg
     2941 catcggaggc gtacgatcgg ctggtgcgca aaggcgcctc ggtcatgtac aaagccgggc
     3001 gtctggccac gtaccagcag gccgaggaga ccctgcttgc cgatgcgccg atcatccccg
     3061 tgtacttcta tacgcggcca tacctgaagt cgccggacgt gaagaactgg cagcccaatc
     3121 acctcggata catctcgtgg aaaacgctct acctggaagc gccggaagag gagtccccat
     3181 aatgccgggt ttccgggtgg ggcacggaac gcagctgcta tactccctcg tatctgcaat
     3241 cagcgggaag ctccgttcat ggtgaaatac atcattcgcc gtctgctggg gtttgtgccc
     3301 acacttttta ttattgtgac ggttgtgttt tttttgattc gtctggcgcc aggcgggccg
     3361 tttgacgccg agcggaatgt gtccagtgag gtgctccaga acctcgaagc ctattaccat
     3421 ctcgacgcgc cccttcacga gcagtatttc cagtatctgc gggatgtgct gcacggagac
     3481 cttggccctt cctttgagaa acccagccgt acggtgagcg aatggatcgc ctaccggtct
     3541 ccggtatcgc tggaactggg gctgtacggt ctcgccgtgg cgctgatcct gggactcagc
     3601 gcgggggtga tcgcgtcgct gcggccgaat accctgacgg accatgtgcc gatggcgttt
     3661 gccatggcgg gcatttgcgt gccgaacttc gtgctgggcc cgctgctggt gctggtgctg
     3721 tcgctccatc tgggctggct gccggtatcg ggctgggaat actccgaaga caagatcctg
     3781 ccggcgatca cgctgggggt gccctacgcc gcgtacatcg cgcggttgag ccgcggcgga
     3841 atgctcgagg tcctgtcgca ggatttcatc cggacggccc gcgcgaaagg gctgtcggag
     3901 gcgcgggtgc tgttgcgcca tgccttgcgc ggcggcattc aaccggtggt ggccttcctg
     3961 ggcccggcga cggcggggtt gatcacgggc tcgtttgtgg tggagaacat ttttcaagtc
     4021 ccggggctgg gcagtgaatt cgtgggggcg gccttgcacc gcgactacac gatgattctg
     4081 ggcacggtgt tgtttttcgc ggcgctcatc ctgttgttca acctgctcgt ggatgtggtg
     4141 ctggcgtggc tggatccgag gctgcgttat gagtgattca cgcaggctgg agaatttcgt
     4201 gccaccggtt gccgtggagc agggcgcgtc gctgtggcgc gacgcgctcg tgcgcttgcg
     4261 caagaaccgc ctcgcgacgg ccgggctggt ggtgctgtgt gcgctggttc tggtgtcgct
     4321 gctgacgccc tggattgcgc cgtacggcta cgaagagcag gatctggcgc tgggggcggt
     4381 tccaccgaac cgcgcgcact ggttcggcac cgacctgctg ggccgcgacc tgttcacgcg
     4441 ggtcctgtac ggcggccgca tctcgcttct ggtggggttc gcggcgacgg gggtgtcgct
     4501 gtgcg
//
LOCUS       DAOPBQ010000294         4494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_15987_length_4494_cov_4.531651, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000294 DAOPBQ010000000
VERSION     DAOPBQ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15987_length_4494_cov_4.531651"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKW60_09845"
     CDS             complement(<1..463)
                     /locus_tag="PKW60_09845"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HPJ99775.1"
                     /translation="MTDPEEHRAENGCRPSEAAREGAYDEQLRFDSPLMRPPAPVRMV
                     IKRDGREEPYDKNKIAAAILRAADAAGEGDRDRAMSLASGVTIFLSKRYEGRAPTVAQ
                     VQDAVEKVLLEMGHARTALAFARHRRKRTRVTRLRQGDLQHLLDEFDEAQWE"
     gene            complement(606..1055)
                     /gene="nrdR"
                     /locus_tag="PKW60_09850"
     CDS             complement(606..1055)
                     /gene="nrdR"
                     /locus_tag="PKW60_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPJ99776.1"
                     /translation="MRCPFCSHTEGKVVDSRSSKEGDSIRRRRECLRCGRRFTTYERI
                     EEIAQMVIKKDGRREAFDRWKIKSGILKACEKRPVSLEQVDSLIDGVERALFNSSDNE
                     ISTEAIGAAVMDRLKDIDQVAYVRFASVYRQFKDLNEFMDELKGLLI"
     gene            complement(1143..1832)
                     /gene="rsmD"
                     /locus_tag="PKW60_09855"
     CDS             complement(1143..1832)
                     /gene="rsmD"
                     /locus_tag="PKW60_09855"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPJ99777.1"
                     /translation="MRQGARWCALYILEFYASYARSWPNWQSYPHRLLPCGATSQKVF
                     FMRIIAGKAKGMRLCAPKGMKVRPTLDRVRESLFNILGPRVSLSRFLDLYAGSGANGI
                     EALSRGASCAVFVDKDLQYIRPNLQKTRLDSLAVCVQATLPGGLEKLGNLPPFDIIYA
                     DPPFKMVDFAPLLTRIAQHALLAAGGVIVLEHASRLTLEERFGPFQRDRVRTYGETQL
                     SFYLDAPAPIC"
     gene            complement(2064..3569)
                     /locus_tag="PKW60_09860"
     CDS             complement(2064..3569)
                     /locus_tag="PKW60_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPJ99778.1"
                     /translation="MAEHIVGIGGERSISEAVAIAAAELAVAEGQPKKIKLRADTCEI
                     MAERADYESPFTTYTLRIRSEEIGTKALQQALAPVSGKYDLTQDDGWQVLRWQETAAE
                     PGEAQQAEDRKFASALHLPEVWRIHGADFRVDNISPHLLFQAMVQYKASDVHLFPGSP
                     PVFRVDNKTQESKLIGALSAHQIRALIREIAADRYWEEFERDFQTSFNYHQTGLGYAR
                     VSAFTKNSAPHCTFRFLPEKIPSFEELNVPHDTMVQLGKLHHGLVLITGMTGSGKSTT
                     MAALVDWINANKAVHILCIENPVEYVHANRKAVVSQRSTGADVRNFFDAVTGALRHDP
                     DVIVIGEMRDPDTIRAAINAAATGHLVISTLHSNTASEVVNRVVSFFDPIERDLVKLQ
                     LRDCIRCVMCQRLVPRIGGGRVPTLEFLFNDIKPITDAIIAGNTDALRIGMQQTVSHS
                     FLFEEYLYKLYKRKIISLENAREFATDVSVLDQMLMGTYSVPRLDSIKAAH"
     gene            complement(3826..>4494)
                     /locus_tag="PKW60_09865"
     CDS             complement(3826..>4494)
                     /locus_tag="PKW60_09865"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPJ99779.1"
                     /translation="GAKVSDIQAWNNMGRKTRIQVGSTLKVGAAGTKVASAPQPKAAE
                     PAAEAHTHVVRPGEYPAKIAKQYGVSLDEFLAWNSLNKDTMIRVGDKLVVHGKVKTAA
                     ASASEPAPSEPVKTVYKVQPGDSASVIADKHGVKLSELLKWNNLSRTSTLKAGSELVL
                     YKASAPRAPAKEEVVVHRVAKGQNPTTIARRYGVSVDDLLRWNGWDKNRVLRVGDTVT
                     IRQN"
BASE COUNT          860 a         1232 c         1417 g          985 t
ORIGIN      
        1 gctcccactg ggcctcgtca aattcatcga gaagatgctg gaggtcgccc tggcgcaacc
       61 gggtgacgcg ggtccgtttg cgccggtggc gcgcaaaagc cagggcggtt cgcgcgtggc
      121 ccatttccag gaggactttc tcgaccgcat cctgcacctg ggcaaccgtg ggcgcccggc
      181 cttcgtagcg tttgctgaga aaaatggtta caccggacgc gagactcatg gcgcggtcgc
      241 ggtctccctc gccggcagcg tccgctgccc gcaggatcgc agcggcgatc ttgtttttat
      301 cgtagggctc ttcgcggcca tcgcgcttga tgaccatgcg gacaggcgcc ggtgggcgca
      361 tcagcggaga gtcaaaccgc aactgctcgt cgtacgcccc ttcgcgcgcc gcctccgagg
      421 gccggcagcc gttttcggca cggtgttctt ctggatcagt catctgtttc cgttgcgaaa
      481 tggcgcaggc aatgccccgc ggcgttcaga tacagtgcaa tggcgtgtaa ggcatcagtt
      541 tagcggcttt tgaccggaga cacaagcgca agcgcggcgg tacattcggg ccggccggca
      601 ggctgtcaga tgagcaaccc cttcaactca tccatgaatt cattcaagtc tttgaactgg
      661 cggtagaccg aggcgaagcg gacataagcc acttggtcga tgtctttgag ccgatccatg
      721 acggcggccc cgatggcttc tgtggaaatt tcgttgtcgg aggagttgaa cagtgcgcgc
      781 tcgacaccgt cgatgaggga atcgacctgt tcgaggctga cagggcgttt ttcacaggct
      841 ttgaggatgc cgctcttgat tttccagcgg tcaaaggcct cgcgccgccc atccttctta
      901 atgaccatct gggcgatctc ttcgatgcgt tcgtaggtgg tgaaacggcg gccgcagcgg
      961 agacattccc gccggcggcg aatggaatcg ccttccttgg atgaccggga atccacgact
     1021 ttgccttcgg tgtgactgca gaatggacaa cgcatggggg atcgccttcc ggtacttacc
     1081 ttaatgctac cacaagagat tccaattttc ggcgctcaac aactatatgt tgtgggatga
     1141 ctttaacaaa ttggggcggg agcgtcaaga tagaaggaca gctgagtttc accgtacgtg
     1201 cggacgcggt cgcgctggaa gggcccgaag cgctcttcca gcgtgaggcg ggacgcatgt
     1261 tcgagcacaa tgaccccgcc ggcagccaga agtgcgtgct gggcgattcg ggtcaggagt
     1321 ggggcaaaat caaccatttt gaacggagga tcggcataga tgatgtcaaa gggggggaga
     1381 tttccgagtt tttcaagccc ccccggaagt gtggcctgca cgcagacagc cagggaatcc
     1441 agccgggttt tttgcagatt gggccggata tactggagat ccttgtcgac aaagacggca
     1501 caggacgcgc cacggctgag cgcctcgatg ccgttcgccc ctgagccggc ataaagatcc
     1561 agaaatcggg acagcgaaac ccggggtccg aggatgttga acaggctttc acgcacgcga
     1621 tccagggtag gccggacttt catgcctttc ggtgcacaaa gccgcatccc tttggctttt
     1681 ccggcgatta tgcgcatgaa aaacaccttt tgtgaagtgg cgccgcaggg cagaagccgg
     1741 tggggataac tctgccagtt tggccaagat cgggcgtaac tggcataaaa ttcaagaata
     1801 tatagggcac accacctcgc tccctgtcgc attgctccgt ctgagtggcg cagatcggca
     1861 aaagtgacgt aacttgcgtg ttttcaacag gatacagacg ccggcctggc cggatggtat
     1921 ccggtttcca aaatcgggaa atttgcggcg aagacttcag gattttatcg attgtctgtt
     1981 tttgggcgcg gccatccccg tgtggaaaac ctgtggataa ccttcttctt cgcccagcca
     2041 gtatcactcc ggaccgttcg gcgtcaatgt gccgctttga tggagtcgag gcgcggcacg
     2101 gaatacgtgc ccatgagcat ctggtcgagc acactgacat cggtggcgaa ttcccgggcg
     2161 ttttcgagac tgataatttt ccttttgtag agtttgtaca ggtactcctc gaagaggaag
     2221 gaatgcgaca ccgtttgctg cataccgatg cgcagggcat ccgtattgcc ggcaataatg
     2281 gcgtcggtaa tgggtttgat gtcattaaac agaaattcca gcgtcggaac ccggccgccg
     2341 ccgatgcggg gcacaagccg ctggcacatg acgcagcgga tgcagtcgcg caattgcagc
     2401 ttgacgaggt cgcgctcgat aggatcgaag aagctgacga cccggttgac gacctcggac
     2461 gcggtattgg agtgcagggt gctgataacc aggtgcccgg tggcagcggc attgatggcc
     2521 gcgcggatgg tgtcgggatc gcgcatttca ccgatgacga tgacgtcggg gtcgtgccgc
     2581 agggcgccgg tgaccgcgtc gaagaaattg cggacgtcag cgccggtgct gcgctgagag
     2641 acgacggctt ttctgttggc atggacgtat tcaaccggat tttcgataca gaggatatga
     2701 accgctttat tggcgttgat ccagtcgacg agggcggcca tggtggtcga ttttccgctg
     2761 ccggtcatgc cggtaatgag gacgagcccg tggtgcagct tgcccagctg gaccatggta
     2821 tcgtgaggga cattgagttc ctcgaacgac ggtattttct cgggcaggaa ccggaaggtg
     2881 cagtgggggg cgctgttttt ggtgaaggcg gaaacgcgtg cgtatccgag cccagtctga
     2941 tggtaattga aactggtctg aaaatcgcgc tcgaactcct cccagtatcg atccgcagcg
     3001 atttcacgga tcagggcgcg tatctgatgc gcggataacg ctccgatcag tttggattcc
     3061 tgggtcttgt tgtccacgcg aaacaccgga ggtgagcccg ggaagagatg gacgtcgctg
     3121 gccttgtatt gcaccatggc ctgaaaaagg agatgaggac tgatattgtc gacgcggaaa
     3181 tcggcgccgt gaatacgcca gacctccgga aggtgcaacg cgctggcgaa tttcctgtct
     3241 tcggcttgct gggcttcgcc aggctcggcg gccgtttcct gccagcgaag cacctgccac
     3301 ccgtcgtcct gggtaaggtc gtactttccg gacaccgggg caagggcctg ctggagggcc
     3361 tttgtgccga tctcttccga acggatccgt aaagtgtacg tggtaaacgg actctcataa
     3421 tccgcacgct cggccatgat ttcgcaggtg tctgcccgca acttgatttt cttgggctgc
     3481 ccttcggcga cggccagctc cgccgcggca atggccaccg cctctgaaat actacgttcg
     3541 ccaccgattc cgacaatatg ctctgccata cgccgaaccc tccttcggaa cgttcagaga
     3601 tcctgcctgc cggtgcgcaa aaggccgccc aacgtatcgg gcgctactat acccctcgtg
     3661 cttgaacagt gtcaaagtat ctggttgcct gtcatgcgca aatcagatgg agacgcctct
     3721 gcagggactt ggtgagagca aaagcgcagg aggcctgcga tatatgcggc ggcccggcca
     3781 ggcggcgggc gctcggcctc cgttgcactg gcgatgtttc gcggcttagt tctggcggat
     3841 ggtgacggtg tcgcccacgc gcaggacgcg gtttttgtcc cacccgttcc accggaggag
     3901 gtcatctacc gagaccccat atcggcgcgc gatagtcgtg gggttctggc ctttggcgac
     3961 gcgatgcaca acgacttctt ccttggcagg tgcacgaggt gcgctggctt tgtagaggac
     4021 cagttccgag cctgccttca aggttgaagt gcgggagaga ttgttccatt tcaagagttc
     4081 ggagagcttc acgccatgtt tatccgcgat gaccgaagcc gaatcgcctg gctgcacttt
     4141 gtaaacggtt ttgacgggct cgctgggcgc gggctccgat gcggacgcgg ccgcggtctt
     4201 cacttttccg tggacgacga gtttgtcacc cacgcgaatc atggtgtctt tgttgaggct
     4261 gttccaggcg aggaattcat caaggctcac gccgtactgt ttggcgattt tggcgggata
     4321 ttcgccgggc cgtacgacgt gggtgtgggc ttcggccgcg ggttcggctg ctttgggttg
     4381 aggtgcggac gcgaccttcg tgccggccgc gccgactttc agcgtggagc caacctggat
     4441 tcgcgtcttt cggcccatgt tgttccaggc ctggatgtcg ctcactttgg ctcc
//
LOCUS       DAOPBQ010000295         4468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16087_length_4468_cov_4.431906, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000295 DAOPBQ010000000
VERSION     DAOPBQ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16087_length_4468_cov_4.431906"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PKW60_09870"
     CDS             <1..1203
                     /locus_tag="PKW60_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99780.1"
                     /translation="RAFSVAAGAARAVDPNHLNLGIRMINAPLPEPGILAAMGKHCDV
                     ISMNLYSMYSDRLPVQMFTMVPAIHAFTGRPTMTTEFSFRGADTLHPNTMGALPTVKS
                     QAERAVGYLSYVAAAASIPSHIGVSWYKYPDDDLEKPWDRYAEDCNFGVIDPAQRPYA
                     VLTEAMRAVNAVIYDLAAEPERNENNPLFWRTELTRWDLGQDEVLLGRLARTNQPFKD
                     PLVLQLPEPRRYHQEYWVHHEGPRLVINDNRFTGWCQANMIEQRDDATVLTLINVLAY
                     TTFPRELWLGRHCAQPGDPIILETNVQFLSRRVAPGGDVLRLTLADGSFIRTDYDQPE
                     LRLDRRVPYLDLRYDHAARQLAVTLRGSATRLGVAGVDGWEVLYNEKPLAPEKISVEG
                     PLSVFALD"
     gene            complement(1310..2203)
                     /locus_tag="PKW60_09875"
     CDS             complement(1310..2203)
                     /locus_tag="PKW60_09875"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPJ99781.1"
                     /translation="MPFIQGGVIPALLTPFTKGGKEVDFERAAGLAEFLADKGVNGLF
                     VAGTTGEGMLMSLDERKQLLETVVKAVGTRVDVIAHTGCMDTASTINLTCHAAQAGAK
                     AAGVVAPWFFGYDNQALEGHYRAVAEAAKGFPIMFYNLPACARNALTAPFIVDLAGKV
                     ENIRGLKDSSGDFLHLGEVLAHAPKGFMVINGVDNFCFQAMVTGANGSVASAANVVPE
                     LFLAIIENVRKNDLANAWEYQKKLSATAKLLRYGAMVALYKEAVRLRGFDAGYVRLPQ
                     RELTAQEKTDFAKAMEALGLI"
     gene            complement(2235..3620)
                     /locus_tag="PKW60_09880"
     CDS             complement(2235..3620)
                     /locus_tag="PKW60_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HPJ99782.1"
                     /translation="MKSALLLAAGEGKGAWPFCGIRQKCTVPIVNVPMVRRMVLDLIA
                     LDVTEIVVVLGSRGEAVRSCLADLAGVRFVEQRPLKGPADAALCGIDAVTGQDTLVCY
                     GDIVTTRDTLRGFLEAYRSRKARAMLLTAPCPAEAPHWVTVHTGEDGLVLDVIGHGDR
                     AQPRFAGIAAARTETLKTYLLRNPGLFTNVEIGAMPPPEGDVAHSLGLMVQDGIEVHA
                     YAAPDFVVDVDRAWQIAQANMEAGRHATEAIQSTVLGEGARIDDSAEIAEGARLILGP
                     GARIGKGCQIGGSMILGKNTQVLNGAIVGAGVCVGNDTQIRDYCSVNDYAVIGSKSLV
                     KHDAEFGGVMFDVVYLYHYCCITALLGNNVDIGAATVCGTLRFDNGIKTQINKGHKEV
                     PDPRYGDLTIMGDYSRTGVNVMFMPGVKVGYYSCVGAGAIVYEDVPERTLLLPKQEHI
                     LKPWGPERYGW"
     gene            3712..>4468
                     /locus_tag="PKW60_09885"
     CDS             3712..>4468
                     /locus_tag="PKW60_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99783.1"
                     /translation="MKPQKTTFALFFGNRGFFPSKLMKEAREEFPRILEQQGYGCLMM
                     DESATRHGAVSTAEEGQIYANFLRENRGKYQGVILSLPNFGDETAAVTALSEADVPIL
                     IQAYPDDLDKMAPAVRRDSFCGKFSIMDLFFQYGLKFTALKPHTVHPACDRFKENLDH
                     FAALCRVVDGIRGMTVGAIGARTTAFKTVRIDEVALQRYGITMETIDLSHVFHRMKDI
                     KPDAAYKDTQERLEQAGSWEGVPEGVLDTVIRLG"
BASE COUNT          856 a         1443 c         1292 g          877 t
ORIGIN      
        1 cgcgccttct cggtggcggc aggcgccgcc cgcgccgtcg atccaaatca cttgaacctg
       61 ggcatccgca tgatcaacgc gccgcttcct gaaccgggca tcctcgccgc catggggaag
      121 cactgcgacg tgatcagcat gaacctctac agcatgtatt cagaccgcct gccggtacag
      181 atgttcacga tggtgcctgc catccatgcc ttcacgggcc gccccaccat gaccaccgaa
      241 ttttcattcc gcggcgccga taccctgcac ccgaacacca tgggagccct gccgacggtc
      301 aaatcccagg ccgagcgcgc cgtgggatat ctgtcgtatg tggccgccgc tgcgtccatc
      361 ccaagtcata tcggcgtctc gtggtacaaa tatcccgacg acgacctgga aaaaccctgg
      421 gatcgctatg cggaagactg caatttcggc gtgatcgacc ccgcacaacg gccctatgcg
      481 gtgctcactg aggccatgcg cgcggtgaac gcggtgatct acgatttggc cgcggagccc
      541 gagcgcaatg agaataaccc cttgttctgg cggacggagc tgacccggtg ggatctggga
      601 caggacgaag tgctgctcgg acgcctcgcg cggaccaacc agcctttcaa agacccgctt
      661 gtgctgcaat tgcccgaacc tcgacgctac caccaggagt actgggttca tcatgagggt
      721 cctcgcctgg tcatcaacga caaccgtttt accggctggt gccaggcgaa catgatcgag
      781 cagcgcgacg atgcgactgt cctgaccctg atcaatgtgc tcgcctacac cacgttcccg
      841 cgagaattgt ggctgggcag gcactgtgcg caacccgggg acccgattat cctggagacc
      901 aacgtgcagt ttctttcccg gcgcgtggcg cccgggggag acgtgctgcg gcttaccctg
      961 gccgacggca gctttatccg gaccgattac gatcaaccgg agctgcgtct ggaccgccgc
     1021 gtgccctatc tcgatcttcg ctacgatcat gcggcccggc agctggccgt caccctgcgc
     1081 ggcagcgcca cccggctcgg agtggcgggc gtcgatggct gggaggtcct gtataacgaa
     1141 aagcccctcg caccggaaaa aatctccgtg gaggggccgt tgagcgtgtt cgctttagac
     1201 tagacgattg gcgtttcgcc acaaaaaaaa ggacaggccg ccgccagcat ttggcgttcg
     1261 gcctgtcctc tctgtcggtc tgttcgggtg cggttggccg tgagctcgct taaatgagcc
     1321 cgagcgcttc catcgccttg gcgaaatcgg tcttctcttg cgccgtcagc tcccgttgcg
     1381 gcaggcgcac gtatcccgcg tcgaacccgc gcagccggac ggcttccttg tagagggcta
     1441 ccatggctcc gtaacgcagg agcttggcgg tcgcgctcag tttcttctgg tattcccagg
     1501 cgttggcgag atcgttcttc cgaacgttct cgataatggc caggaagagc tccggcacaa
     1561 cgttggcggc actggcgacg ctgccgttgg cgcccgtcac catggcctgg aagcagaagt
     1621 tgtctacgcc gttgatgacc atgaagcctt tcggagcatg ggccaggacc tcgcccaggt
     1681 gcaggaagtc gccgctgctg tctttcaggc ccctgatgtt ttcgaccttc ccggcgaggt
     1741 caacgatgaa cggcgcggtc agggcattgc gcgcacacgc cggcaggttg tagaacatga
     1801 tcgggaatcc cttggcggcc tccgccacgg cccggtaatg cccttcgagc gcctggttgt
     1861 cgtagccgaa aaaccacggc gccaccacgc cggcggcttt ggccccggcc tgcgcagcat
     1921 ggcaggtcaa gttgatcgtg ctggccgtgt ccatgcagcc ggtatgcgcg ataacgtcta
     1981 cccgagtgcc taccgccttc actacggtct ccaggagctg cttgcgttcg tcgagggaca
     2041 tcaacatgcc ttcgcccgtg gttcccgcga caaagagccc gtttaccccc ttgtcggcaa
     2101 ggaattccgc gaggcctgcg gctctttcaa aatcaacctc tttcccgccc ttggtgaacg
     2161 gggttaatag tgctggaatt acaccgcctt gaatgaatgg catgtccgtc tcctctttcc
     2221 cggctggatt ttatctacca gccgtaacgt tcgggtcccc acggtttcaa aatatgctcc
     2281 tgtttcggaa gcagcagcgt tcgttcgggc acgtcttcgt acacgatggc gcccgcgcct
     2341 acgcaggagt aatagcctac cttcacgcca ggcatgaaca tcacgttcac ccccgtgcgc
     2401 gaataatcac ccatgatcgt cagatcccca taccgcggat cgggcacttc cttgtggcct
     2461 ttgttgatct gcgttttgat gccgttgtcg aatcgaagcg taccgcagac cgttgcggcg
     2521 ccaatatcca cgttgttgcc cagcagtgcg gtgatgcagc agtaatgata caaatacacc
     2581 acatcaaaca tgaccccgcc gaactctgcg tcatgtttca ccagggattt cgaaccgatc
     2641 accgcatagt cgttgacgga acagtaatcg cgaatttggg tgtcgttgcc cacgcatacg
     2701 cccgcaccca cgatggcgcc gttgaggacc tgcgtgttct ttcccaggat catcgaaccg
     2761 ccgatctggc agcccttgcc gatccgcgcg ccaggcccca ggatcaggcg ggcgccctcg
     2821 gcaatctccg cgctgtcgtc aatgcgggcg ccttcaccca gcaccgtgct ctggatcgcc
     2881 tcggtggcat gccggcccgc ttccatgttc gcctgcgcaa tctgccaggc gcgatcgaca
     2941 tcgaccacaa aatccggcgc ggcataagcg tgcacctcaa tgccgtcttg caccatcagg
     3001 ccgagactgt gggctacgtc gccttcagga ggcggcattg caccaatctc gacattggtg
     3061 aagagcccgg gattgcgcaa gagataggtc ttgagcgtct ccgtgcgagc cgctgcaatc
     3121 ccggcgaagc ggggttgtgc acgatcaccg tgaccgatca catccaggac cagaccgtct
     3181 tcccccgtgt gcaccgtgac ccagtgcggc gcttccgccg ggcacggcgc cgtcaggagc
     3241 atcgcgcggg ctttccgcga ccggtaggcc tccagaaatc cccgaagggt atctctcgtg
     3301 gtcacgatgt cgccgtagca gacgagcgtg tcctggccgg tcacggcatc gatcccgcaa
     3361 agggcagcgt cagccggacc cttgagcggg cgctgctcca cgaaccggac gcccgccaga
     3421 tcggccagac aactccggac cgcttcgccg cggctcccga gcacgacgac gatttctgtc
     3481 acgtcgaggg cgatcagatc cagcaccata cgccgcacca tcggaacgtt cacaatcggc
     3541 acagtgcact tctggcggat tccgcagaag ggccaggcgc ccttaccttc gccagcggcc
     3601 aacagcaatg cggatttcat ggttgatgtt tcctctctgg aaggcgtgct cccctgacag
     3661 tatacttatc gacatgcgga ctggcaacgc acacaccgga aggaagatgt catgaaaccc
     3721 caaaaaacca catttgcgct ctttttcggc aatcggggct ttttcccgtc gaaattgatg
     3781 aaggaagccc gtgaagaatt tccgcggatt ctcgaacagc agggctatgg ctgcttgatg
     3841 atggacgaga gcgccacgcg ccatggcgcc gtctcaaccg ccgaggaagg ccagatctat
     3901 gccaatttcc tccgcgaaaa ccggggcaag taccagggag tcatcctgtc tctacccaat
     3961 tttggcgacg aaaccgccgc cgtgaccgcg ctcagcgagg cggatgtccc catcctgata
     4021 caggcctatc cggacgatct cgacaaaatg gccccggccg tgcggcggga ctcgttctgc
     4081 ggaaaattct cgatcatgga cctgtttttt cagtatgggc tgaaatttac ggcgttgaag
     4141 ccgcataccg tgcaccctgc ctgcgaccgc ttcaaagaga atctcgacca cttcgctgcc
     4201 ctgtgccggg tggttgacgg cattcgcggg atgaccgtcg gcgcgatcgg ggcgcgaacc
     4261 acggcgttca aaaccgttcg catcgacgag gtggccctgc aacgctacgg catcaccatg
     4321 gaaaccatcg atcttagcca cgtgtttcac cggatgaaag acatcaagcc ggacgcggcc
     4381 tacaaagaca cccaggagcg gctcgaacag gccgggtcgt gggaaggcgt gcccgaaggc
     4441 gtgctcgaca cggttatccg gctgggcc
//
LOCUS       DAOPBQ010000296         4465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16100_length_4465_cov_3.776871, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000296 DAOPBQ010000000
VERSION     DAOPBQ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16100_length_4465_cov_3.776871"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PKW60_09890"
     CDS             <1..733
                     /locus_tag="PKW60_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPJ99784.1"
                     /translation="QPLTIALEDAMTADDFWAAVSSSGAVLKRRQGCGGADAQFIRHL
                     EELQPYLESLEKDRWILQEYVEGVDIAVNFVSKSGNFIAWFVQEALSTLGQFGYQMHM
                     RISTEHTEAVAMAQRVVDALEWEGPGCVDFRVDKRTNQLLCFENNPRFGRAVLIGTVA
                     GVNLPLAAAGRPVPDVEAQVAEFQHLSNLYRSPTQLFRPWAQNGIKRRTILPEILRDP
                     IPEMAQSFTRFVSQLSPRGRNAARG"
     gene            complement(730..>4465)
                     /locus_tag="PKW60_09895"
     CDS             complement(730..>4465)
                     /locus_tag="PKW60_09895"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ99785.1"
                     /translation="AKKRKLDEALELATYAVDLAPQDRENHLLLFSLMERAGKPADEL
                     LAYAEKIASIAPTELENHFLVLAKMQQAGRPGEELLAYATAQQDKLASQEGLLQVLQA
                     TAHEIAGNHEDARRLLEEAAEHISPDPRIVSLVNNFLNRVGSFDVSTKLLERAVAESG
                     DDRLRLLLVRQLFERGNSGAVLEKTGGMPLEQLDSPLLAIRALACAAEHQTEQVNAIA
                     GEFDRREGDAAARAWAPLLRAVWGGEEAPPAKVMTLCDEAIGLQPDNPFLHYLRAVAC
                     ERLGESDLALSSWQDARQYAPSWAEPAIRAASLLDSMGNRAEAVRLATQALKMASNNI
                     GVAAEAAVIIGSDPDWLSDDGRQQLLKLVEQVQTARPLEPRTLPLYVDLLARNGDTAG
                     AADKIRQALNSQETLPDAELLQLARTSDAHGLDLADACYEKAASSSAITPAVAFAKAV
                     AAYNAGNGDKGLEILANAAKAAENTPGWETVLPEYLERTGAPDATEAWAKAADQHPGA
                     AAVQRRVLASRAAWQDTDLIGRVIERVKEQTGEEAVGWRVARARWLLMTDSSEKAAAE
                     AVALLNDVIRTSAPQADRHVLLASAFERLNNYEGAQYSLTNALQIAPESNAIRFQLAR
                     ISQIRGQSDRARQYIEDALRNPAVSAGDRQRAAAIFASQNEIGRAIALQLEAHPPEDR
                     ENPADLLLAELYRRSSMPDKAEAICTRILQEAPTAQAVQFTADLLASQGRTQEAETVL
                     ARLDTLETEPGIRELIQADYHSRYGTSEAARRQFEAALAARRNDPAVWKRYLVFLARS
                     GAAAEAVGLFSDAAAACPNEPVFAQLAEDSATLSAAANRAIALPLIVAAIEAPRRHDT
                     TFEALNAIANTPENNPERLAMQLRQLSERHPEFLALKMQLVRLYASINRHDDAAKVAA
                     QAMHDFPGEYEPALLAAEAYAATGQWMESLRAAEAWRNRAVGRAVGADMLIAEAQIQL
                     GEGSQALETLRSYVDSPGETEQTGYSPALAQQARALISAGQPAEAAALIQPRLTASAD
                     SADAQRAAAQWRMAWVRLAASEIKDDTTAAEWLLQVTPRIPDAELGERLMLASGWYTL
                     ANRSADPDNSSRQSARAILQTLADRPGADAKAFFELAVVADLGKDYAQAETYYLRALD
                     ADPAFAVAQNNLAMRYVLEERRLDEALKLAQAAVQAGPDNANYLDTLAQAQAALGNYD
                     DAIQALTRAAELDPNQRKWRDNIEVIKQRQKQTSSGARES"
BASE COUNT          712 a         1273 c         1542 g          938 t
ORIGIN      
        1 gcagcctctg actatcgcgc ttgaagatgc catgacagcg gacgatttct gggcggcggt
       61 ctcctcttcg ggggcggtcc tcaaacgcag acaagggtgt gggggagcgg acgctcagtt
      121 tatacgccat cttgaggaat tgcagcctta tctcgaatct ttagagaaag atcggtggat
      181 cctgcaagaa tacgtcgaag gtgtggatat cgccgtgaat tttgtcagta aatccggcaa
      241 tttcatcgcg tggtttgtgc aggaggcgct cagcactctg ggtcagttcg gataccagat
      301 gcacatgcgg atcagcaccg agcacactga agctgtcgct atggcgcagc gtgttgtaga
      361 cgctttggag tgggaggggc cggggtgcgt tgattttcgt gtggacaagc gcacgaacca
      421 gcttctctgc ttcgagaaca acccgcgttt tggccgggcc gtattgattg gcactgtggc
      481 gggggtgaac ctgcctcttg cggcggccgg gcgccctgtg ccggatgttg aggcccaggt
      541 ggctgaattt cagcatctct caaacctgta tcgctcacca acgcagctgt ttcggccgtg
      601 ggctcaaaat ggcatcaagc ggcggacgat tcttccagag atcttacggg atccgattcc
      661 ggagatggcg cagagcttca cccgcttcgt gagccagtta tctccccgtg ggagaaacgc
      721 agcgcgcggc taactttccc tggctccgct gctggtttgc ttctgccgtt gtttaatgac
      781 ctcgatgttg tcgcgccatt ttcgctgatt cgggtccaat tcggcggctc gggtgagggc
      841 ctggatggcg tcgtcgtagt ttccgagggc agcctgggcc tgggcgagcg tgtcgaggta
      901 attggcgttg tcgggtccgg cctggacggc agcctgggcg agtttgaggg cctcgtcgag
      961 gcggcgctct tcaaggacgt aacgcatggc caggttattc tgggcgacag cgaatgcagg
     1021 gtcggcgtcg agtgcgcgga ggtaataagt ctcggcctgg gcatagtctt tgccaagatc
     1081 cgcgacgact gcgagttcga agaaggcttt tgcatcggcg ccaggccggt cggcgagggt
     1141 ctggaggata gcgcgggcac tctggcggct ggagttgtcg gggtcggcgc tgcggttggc
     1201 gagggtgtac catccggagg cgagcatgag gcgttcgccg agttcggcgt cggggatgcg
     1261 cggggtgacc tggagaagcc attcggcggc ggtggtatcg tccttgatct cggaggcagc
     1321 aaggcgtacc caggccatgc gccattgggc ggcggccctt tgggcgtcgg cggaatcagc
     1381 tgaggcggtc aggcgcggct ggatgagcgc ggcggcctcc gcgggctgtc cggccgagat
     1441 gagagcccga gcctgctgcg cgagggctgg tgaatagccc gtctgttcag tttcgccggg
     1501 gctgtcgaca taggagcgaa gcgtttccag ggcctgagag ccttcaccga gctggatttg
     1561 tgcttccgcg ataagcatat cggcgcccac ggcgcgtccc acggcgcgat tgcgccaggc
     1621 ttcggcagcg cggagagatt ccatccattg gccggttgcg gcgtaggctt cggcggcgag
     1681 cagggcgggc tcgtattcgc cggggaagtc gtgcatcgcc tgggcagcga cctttgcggc
     1741 gtcgtcatgc cggttgatac tggcgtagag gcggacgagc tgcattttca gcgcgagaaa
     1801 ctcggggtgg cgttcggaga gctggcgtag ttgcatagcc aggcgctccg ggttgttctc
     1861 gggggtattt gcgatggcgt tgagtgcctc gaaggtggtg tcgtggcgcc ggggcgcctc
     1921 gatggccgcc acgatcagcg gcagtgcaat ggcacggttc gcggccgcgc tgagggtggc
     1981 gctgtcttcc gccagctggg caaacacggg ttcattgggg caggcggccg ccgcgtcact
     2041 gaacaggccg acggcctctg cggcagcgcc gctgcgcgcg agaaaaacga gataccgttt
     2101 ccagacagcg ggatcgttac ggcgcgcggc gagagcggct tcaaactggc gcctggcagc
     2161 ttcggaggtt ccatagcggc tgtggtaatc ggcttggatc agctcacgga ttccgggttc
     2221 ggtctcgagc gtatccagcc gggcgaggac ggtttcggcc tcctgcgtgc gcccctggga
     2281 ggcgagcagg tcggcggtga actgaacggc ctgcgcagtg ggtgcctcct ggaggatccg
     2341 tgtgcagatc gcctccgctt tgtccggcat gctgctgcgc cggtagagct cggcgagcaa
     2401 gagatccgcc gggttttcgc ggtcctctgg gggatgcgct tccagctgga gtgcgatagc
     2461 gcggccgatc tcgttctggg aggcgaatat cgcggcggcg cgttgtctgt cgcctgcgga
     2521 taccgccgga ttgcgcagcg catcttcaat atactgccgt gctcgatccg attgcccccg
     2581 gatttgagaa atgcgcgcca gctggaaacg aatggcattg gattcaggcg caatctgcag
     2641 ggcattggtg aggctgtatt gggcgccttc gtaattattc agccgttcaa atgcgctcgc
     2701 cagtaaaaca tggcgatcgg cctgcggcgc ggaggtgcgg atgacatcgt tcaggagtgc
     2761 gacagcttcg gcggcggctt tctcagagga gtcagtcatc aggagccagc gagctcgcgc
     2821 caccctccag cctacggcct cctcgccggt ttgttctttc acacgctcga tcacgcgtcc
     2881 gatgaggtcc gtgtcctgcc aggcggcccg tgatgcgagc acgcgccgct gcacggccgc
     2941 ggcgccaggg tgttggtcgg ctgctttcgc ccacgcctca gtcgcatccg gcgctccggt
     3001 ccgttcgaga tattcgggca ggacggtctc ccagccgggc gtgttttcag cagccttggc
     3061 cgcgttggcc agaatttcga ggcctttgtc gccattacct gcgttgtagg cggcaactgc
     3121 tttggcaaag gccacggccg gcgtgatggc agaggagctt gcggcctttt cataacatgc
     3181 gtcggccaga tccaggccat gcgcgtcgct cgtgcgggcc agttgtagaa gctcggcatc
     3241 cgggagcgtc tcctgggagt tgagagcctg tctgatcttg tctgccgcgc ctgccgtgtc
     3301 tccgttgcgg gccagcaggt cgacatagag cgggagcgtc cggggctcga gtgggcgggc
     3361 ggtttgaacc tgctcgacca gcttcagcaa ctgctgcctc ccgtcatcgc tgagccagtc
     3421 gggatcactt ccgatgatta cagcagcttc ggcagcaacg ccgatgttgt tggaggccat
     3481 tttcaaggct tgagtggcga gccgcacggc ttcggctctg tttcccatgg aatccaggag
     3541 agaggcggcc cggatggctg gttccgccca ggagggcgcg tactgcctgg cgtcctgcca
     3601 ggaactcaag gcgaggtcgg actcgcccag acgctcacag gcaacggcgc gcaggtagtg
     3661 caaaaaggga ttgtctggtt gaaggccaat ggcctcatca cagagtgtca tcactttcgc
     3721 cggcggggct tcttctcctc cccacaccgc gcgcagcagc ggcgcccatg cccgcgccgc
     3781 cgcatctcct tcccgccggt cgaattcacc ggctatggcg ttgacctgtt ccgtctgatg
     3841 ttccgctgcg caggcgaggg cgcggatggc gagcagggga gaatccagct gttcgagcgg
     3901 catgccaccc gttttttcaa ggacggcacc ggagttccct cgctcaaaga gctggcgaac
     3961 cagcaggagt cgcagccggt catcgcccga ttccgcgacc gctcgttcga gtaatttggt
     4021 cgacacatcg aaactgccta cccggttgag aaagttattg accagagaca caatcctggg
     4081 atcgggggag atgtgttcgg ctgcttcttc aaggaggcgc cgggcatcct cgtgatttcc
     4141 ggcgatttcg tgcgcggttg cctgcagtac ctgcagcaat ccttcctgcg acgccaattt
     4201 gtcctgctgc gcggtggcgt aggccagaag ttcttcgccg ggccggcccg cctgctgcat
     4261 ctttgccaac acgaggaagt ggttttccag ttccgtgggc gcgatggatg cgatcttctc
     4321 tgcataggcc agaagttcat cggccggttt gccggcccgt tccatgagcg agaacagcag
     4381 caggtggttt tcccgatctt gcggcgccag gtcgacggcg taggtcgcga gctccagagc
     4441 ctcatcgagt tttctcttct tggcg
//
LOCUS       DAOPBQ010000297         4465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16101_length_4465_cov_3.414966, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000297 DAOPBQ010000000
VERSION     DAOPBQ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16101_length_4465_cov_3.414966"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            414..1766
                     /locus_tag="PKW60_09900"
     CDS             414..1766
                     /locus_tag="PKW60_09900"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPJ99786.1"
                     /translation="MRLTSREPVKKRIGEMLIDAGLVTREHVEEALAKQRREGGKLVE
                     LLIALGRLQTNEFVSFLARQGGVPSIDLQRYEIPKPIIELLDREFVLKHEVIPIDKLG
                     KLLTVAMVCPLDSATIEELQLMTQCKVKPMLCSPDAIRKAIQRYYPAGGPESGQQAGG
                     AALNVDGLEGALSLRNVTLLVRQIEALPALPETIRRMRESASDSTTSVKEVGKIVSGD
                     PAVAAKILSVANSAAYGFSHRVDNVSHAVSLLGLRETYSLALALSIADIMKESKGFDY
                     KRYWEQAVQCAAASRLLAEEHGRPDNGGTFTAGLLHNLGKLALAHVAPGAYPQIGADL
                     YGQDLIEAEQKVFGLAHPEAGYELALHWELPEHVAEAIRFHHTPEQATKHSEVVSLVA
                     LAVFMVYASMSMDPRDDRFLDEHKDLLRCAGLELERSKTAYHTFLTRLMDLTAEAFLV
                     "
     gene            1974..2642
                     /locus_tag="PKW60_09905"
     CDS             1974..2642
                     /locus_tag="PKW60_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99787.1"
                     /translation="MKTITRVAIPAMAVVLALTVLPVFAQSDPAPQNPNTLLENPGTA
                     ANTPPATEANVSPEPFGQGRGRGLRDGRGMGQGPGLGRGRQGQNIQGEQPAPGTGTEK
                     NPAPGDGRGRGLRDGQGRGQCLRDGSCGCIQGQGMARGRGQGYGRGARGKAGEPAMTP
                     GFGGGRGQGRGLGYGRGQGQGRGQGRGQGQGQGYGRGPRDGSGPGRGLGFGGGCLPDC
                     PNAR"
     gene            2660..4048
                     /locus_tag="PKW60_09910"
     CDS             2660..4048
                     /locus_tag="PKW60_09910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPJ99788.1"
                     /translation="MEPRDNSRNPVILVVLAAALLAAASIFLLVRQYRVEVVNAQVQL
                     LAKGRTTLDALTAGIRAQSRRGMYRAERLAAIFDELAQTPGILALRLRSASGAVVAEG
                     GELLDIPDLPPGAAQWTSDTLIMTASAEFIHDRPGMGLFRRGRGQGGPGLGLGLRRGG
                     GRGLGPDNREDALDAGDTPWEAGPHVLTVVLNTGDVRFAIRYARLRLAGVAFVVLVAV
                     SSGAWAFVTQLHQRRLMTGLLLAEERAAQSERLARLGAGLAHETKNPLGVVRGLAQSI
                     LEAPDAGRTTRGMAQQIVDESDRTVGQINSFLGFARPLEPELRPVDLSRLLDEMKPLF
                     EAEARGTAEITCAGNQITVQADESMLRRALLNLVINGIRAIQHAGTVAVRAERAHDGA
                     RIVVEDNGCGIAPEDLARLPEPYFGRFEGGSGLGLSIVDQIARAHGWRLRFHSVPGMQ
                     TQVSLDGLSEVG"
     gene            4053..>4465
                     /locus_tag="PKW60_09915"
     CDS             4053..>4465
                     /locus_tag="PKW60_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPJ99789.1"
                     /translation="MTEPRYRIAIVDDDPGQRQLLANALERAGYETLRCEDGPQGLEA
                     AAQASLMLLDVRMPGMSGLEVLEHVKRDRPQLPIILLTAYIDVRDAVNAMKLGALDYL
                     EKPVDLDELIAAVDDALGATGRAVSQDGTPEIPEG"
BASE COUNT          877 a         1368 c         1470 g          750 t
ORIGIN      
        1 ggcctttcca gcaccgaagt cgtgctatta tagctgcccg gacagtgcca acgcaaaaag
       61 caccgctgtg cagctccggc tgctcatggc gcatcacaag ccgcttccca ggaaagactg
      121 gtgcccgaac gtgagacgcc ggaacgccag gcggaacggc gccgcggtgg cgcaccgaac
      181 cgggaaaccg ttgtaacgtg acatttctaa cgagttttga cgggattaat tcttgacgag
      241 acaccatagt gaactctaca attataatcc gggatgtttt ggacattgac agtgtccaca
      301 gcatgctatt ttagaagcaa catggggatt gtaaagatgg cggccgacaa ggcactgtca
      361 acaacccaga gccaagtcac acaaccgggt atacagtgcc cgatggatag agaatgagat
      421 taacctctag agaaccagtc aaaaaacgca ttggcgaaat gctcattgat gcaggcctgg
      481 tcacgcggga acatgtcgag gaagcgttgg caaaacagcg ccgcgaaggc gggaagctgg
      541 tcgagctgct catcgcgctt ggccggctgc aaaccaacga atttgtgagc tttctggcgc
      601 ggcagggcgg tgtgccgagc atcgatcttc aacgttacga gatcccgaag ccgatcatcg
      661 aactgctcga tcgcgaattt gtcttaaaac acgaagtcat tcccatcgac aagctgggca
      721 agctgctgac ggtggcgatg gtatgcccgc tggattcggc taccatcgaa gagctgcagc
      781 tgatgaccca gtgcaaagta aagccgatgt tgtgctcccc tgatgctatc cgcaaggcca
      841 ttcagcgtta ctaccccgcc ggaggcccgg agtccggcca gcaggccggc ggagcagctc
      901 tgaacgtaga cggtctggaa ggcgccctga gtctgcgcaa cgtcaccttg ctggtgcgcc
      961 agatcgaggc gctgccggcg ctgcccgaga ccatccggcg gatgcgcgaa tcagcgtcgg
     1021 attccacgac ttcagtgaag gaagtcggga aaatcgtctc gggcgacccc gccgtggcgg
     1081 cgaaaatttt gagcgtggcc aattcagccg cctacgggtt ttcacaccgg gtggacaacg
     1141 tttcgcacgc ggtatcgctg ctggggcttc gtgaaacgta ctctctggcg ctggctctgt
     1201 ctatcgccga catcatgaaa gaaagcaagg gattcgacta taaacggtac tgggaacagg
     1261 cagtccagtg cgctgcggcg tcccggctgc tggccgaaga acatgggcgg cccgacaacg
     1321 gcggcacatt cacggccgga ttgctgcaca atctcggcaa actggcgctg gcgcatgtgg
     1381 cccccggagc gtacccgcag atcggggcag acctgtacgg ccaggacctc attgaagcgg
     1441 agcagaaagt attcggcctg gcccatcccg aagcaggcta tgaacttgcc ctgcactggg
     1501 aactgcccga gcatgtggcg gaagccatcc ggtttcacca cacgcccgag caggctacga
     1561 agcatagcga ggtcgtatcg ctggtggccc tggcggtgtt catggtctac gcctccatgt
     1621 ccatggatcc cagggatgat cgcttcctgg acgagcacaa agatctgttg aggtgcgcgg
     1681 gactcgagct ggaacgatca aagaccgcct atcatacctt cctgacgcgg ctgatggacc
     1741 tgaccgcgga ggcctttctc gtctaaagcg cgcgtggaag gccgcatcaa caccgcgcag
     1801 gaatgcaaga aatgcacagc gcgcccccga atgtgcttct tttgcacagc ggcaggctgc
     1861 aaatgacagt gaaatgaatg gcacgggcct tgcactcttt gcgggtggaa cacaaaaagg
     1921 cccgccctca gaggctatac gagcccacgg ggcgcctgat aaggagagcc accatgaaga
     1981 cgataacacg agttgcgatt ccggcaatgg ctgtggtgct cgcactgacc gtactgccgg
     2041 tctttgcaca gagcgacccc gcgccgcaaa accccaacac gctcctggag aaccccggca
     2101 cggccgcgaa cacgccgccc gccaccgagg ccaacgtttc ccctgaaccc ttcgggcaag
     2161 gccgtgggcg cggcctgcgc gatggacgcg gcatgggcca ggggcccggt ctcggccggg
     2221 gccgccaggg ccaaaacatc cagggcgaac agcctgcccc tgggaccggc actgaaaaga
     2281 acccggcgcc cggcgacggt cgcgggcgtg gtctgcgcga cggtcaaggc cggggccagt
     2341 gcttgcgcga cggcagctgc ggctgcatcc aaggtcaggg tatggcgcgc ggccgcggcc
     2401 agggttacgg gcgcggcgcc cgcggcaagg cgggtgagcc tgctatgaca cccgggtttg
     2461 gcggcggccg tggccagggc cggggcctgg ggtacgggcg cggtcagggc cagggccggg
     2521 gtcagggccg cggtcagggc cagggccagg gttatgggcg cggcccacgc gatggcagcg
     2581 gacctggccg gggtctggga tttggcggag gctgcctccc cgattgcccc aatgcccgct
     2641 gatggtacac tgaagggcca tggaacccag agataacagt cggaacccgg tgattttggt
     2701 ggtgctggca gctgcgttac tcgcggctgc cagcattttt ctactggtcc ggcaataccg
     2761 cgtcgaagtc gtcaatgcac aggttcagct cctggcaaaa ggccgaacca cgctcgacgc
     2821 cttaaccgcg ggcatccgcg cccagagccg ccggggaatg taccgggccg agcggctggc
     2881 ggccatcttc gatgaactgg cccagacgcc ggggatcctg gcgctgcggc tccgttcggc
     2941 cagtggcgcg gtcgtagcag aaggcggcga gttgctggat ataccggatc tgccccccgg
     3001 ggcggcacag tggacctcgg acaccttgat catgacggcg tcggccgaat tcattcacga
     3061 ccggcccggc atgggacttt tcaggcgagg gcggggccag ggcggtcccg gcctcgggtt
     3121 ggggctgcgg cgcggcggtg gacgtgggct tggccctgac aaccgggagg atgcactcga
     3181 cgcgggcgac acgccctggg aagcaggacc gcacgttttg actgttgtgc tgaacacggg
     3241 cgacgtccgg ttcgctatac gctacgcacg cttacggctg gccggcgtgg cgttcgtcgt
     3301 gctggtggcg gttagttcag gcgcatgggc tttcgtaacc caattgcacc agcggcggct
     3361 gatgacgggc ctgctgctgg cggaagaacg cgcggcccag agcgagcggc tggcccgcct
     3421 gggcgcaggg ctggcccacg aaaccaagaa ccctctgggc gtagtgcggg gactggctca
     3481 atccatcctc gaagcgccgg atgccgggcg caccacccgt ggcatggcac agcagatcgt
     3541 cgacgaatcc gaccgcactg tgggacagat caactcgttt ctcggctttg cgcgcccgct
     3601 ggaacccgag ttgcggcccg ttgatttgtc gcgcctgctc gacgaaatga aaccgctgtt
     3661 cgaggccgag gcgcgcggca ccgccgaaat cacatgcgcc ggcaatcaga tcaccgttca
     3721 ggccgacgaa tcgatgcttc gccgcgccct tctgaacctg gtgatcaacg gcatccgcgc
     3781 gatccaacac gcgggcacgg ttgccgtgcg cgccgagcgc gcccacgacg gcgcgcgcat
     3841 cgttgttgaa gacaacggct gcgggattgc cccggaagat ctggctcgcc tcccggagcc
     3901 gtattttgga cgtttcgaag ggggctccgg cctgggtctg tcgatagtag accagatcgc
     3961 ccgggcccac ggatggcggc tgcgattcca ctccgtgccg ggcatgcaga cacaggtatc
     4021 actcgacggc ttgtctgagg taggctaagg acatgacgga accacgctac cgcatcgcca
     4081 ttgtcgacga cgacccggga cagcgccagt tgctggccaa tgccctcgaa cgcgcggggt
     4141 atgagaccct ccgctgtgaa gacggccccc aggggctcga agcggcagcc caggcctcgc
     4201 tcatgctgct cgacgtgcgc atgcccggca tgagcggcct cgaagtcctc gagcacgtga
     4261 aacgcgaccg gccgcagctt cccatcattc tgcttaccgc ctatatcgac gtgcgcgatg
     4321 cggtcaatgc gatgaagctg ggggctctgg actatcttga gaagcctgtc gacctcgatg
     4381 agctcatcgc ggcggtggac gatgcgctgg gcgctacagg gcgcgccgta tcgcaagacg
     4441 gtacgcccga gatccccgag ggcat
//
LOCUS       DAOPBQ010000298         4446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16188_length_4446_cov_4.412435, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000298 DAOPBQ010000000
VERSION     DAOPBQ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16188_length_4446_cov_4.412435"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PKW60_09920"
     CDS             complement(<1..663)
                     /locus_tag="PKW60_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99790.1"
                     /translation="MRTMKILVLVVTAIMVAGAVSAEDSSLVGPGKIFVRFTNTRPDQ
                     SAVVVRLHVVPNNDEPFGWGGVTRYVGAEEAEEKPGVPPLAPGASTPWLDVGQYMNLQ
                     GARSWDTYLSPILCGVQTLPPAPGLYLVAEIAEGPGMNIMRRIEIRKPELAPEVPERD
                     YPWYLGYGVWNHGKPILPTLGLLVPSRPEISPRVYTLEEALNWQLDLIKDFPDTGRLP
                     EKL"
     gene            1107..2072
                     /locus_tag="PKW60_09925"
     CDS             1107..2072
                     /locus_tag="PKW60_09925"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPJ99791.1"
                     /translation="MIDLSKLKNPLDWIQARKDIESSVLKVLGALPKERAELQMKVVD
                     EESFPGYTRRRVNYFVSDWERIAAWMFVPNRKEPVPGIVCCHSEHPEGKSEPAGLAGE
                     RLMALAVHYAEQGYATLAPDCATAGERVSAGKAAYDTSNFYKDWPKGSLLGKMVWDYT
                     CGLYALGDVPGVDSSRLGIIGHGLGGTCALLVAALDERVQVCVASCGFTRFHDDNEVG
                     RWVKEAHCALAPNLKDAVAKKEFPFDWEHVLALAAPTPTLLITALNDAKLLNTKSCQK
                     AKDAARHIYRMLGRDDALENYTHSDGRTLTVDAIDAADSWFERWL"
     gene            2391..3347
                     /locus_tag="PKW60_09930"
     CDS             2391..3347
                     /locus_tag="PKW60_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPJ99792.1"
                     /translation="MPAVDTQVVERIAALVSGVERVIFGKHDTVKLCVTGLLARGHIL
                     IEDVPGIGKTTLAQGLARSIDCKFSRIQFTSDMLPSDILGVTVLNPKTREFEFRPGPI
                     FAGVVLADEINRTPPKNQSALLEAMSESQVSVDGVTRPLPQPFVVLATQNPIEYDGTY
                     TLPESQLDRFMLRVEIGYPGSDDELHIMRRRDPRTAMTQLEPVLSAAEIVALQEQVPD
                     IHVDNSVARYMLAIVQGTRQHEHIQLGASPRAAQEFYEACQAHALVEGRNFVTPDDVK
                     QLAVPALSHRILVRSREGNLAAAARARARAVYDVVKNTPIPR"
     gene            3353..4426
                     /locus_tag="PKW60_09935"
     CDS             3353..4426
                     /locus_tag="PKW60_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPJ99793.1"
                     /translation="MRTRRRSLTWRLPRAGQVFVLMSAVVLLAAWNSGMNLLYLLVGG
                     LVSFLVLSLFFSAKNLKGLKVQFDAPAAVHRGDNAAITVRVENHKAVLPTVSLRIELA
                     ENRGQSVAYLVKLPAGKAGVARINATFPRRGVYPPPPIELVTTFPFGLIETRLRVQGT
                     TEVLVYPRVHSVRPAFLDSVRGTGEMPRRIRGLGDEFFGLRGYMPGDDLRHVAWRVSA
                     RADELIVKEMAHETSRHVQLVFDARERTAQPDFEEQFEEAIELAASLGETLLSRQFRV
                     GFLTAGEYLPADEGKVQSTRLLEMLARLQPEPGSLPDPFLQAVHADDSREATCIFVSP
                     DPAEWGRRIGAGHVMRPEEAVYA"
BASE COUNT          852 a         1272 c         1493 g          829 t
ORIGIN      
        1 gagcttttca ggaagccggc cggtatccgg aaagtctttg atgaggtcga gctgccagtt
       61 gagggcttct tcgagggtat acacccgggg cgagatttcc gggcgcgaag gcaccaggag
      121 ccccagcgtg ggaaggatcg gtttgccgtg gttccagaca ccgtagccca gataccaggg
      181 ataatcgcgc tccgggactt ccggggccag ctccggtttc cggatctcga tccggcgcat
      241 gatgttcatg ccggggcctt cggcaatttc ggcgacaagg tagagtccgg gggctggagg
      301 caacgtctgc acgccgcaca ggatgggcga caggtaggta tcccagctgc gggcgccctg
      361 caggttcata tactggccca catcgagcca cggcgtggat gcgccgggcg cgagcggagg
      421 aaccccgggt ttctcctctg cttcttcggc acccacatag cgcgtaacgc ctccccagcc
      481 gaagggttcg tcgttattgg ggacaacgtg cagacgcacc acgacagcgg actgatcagg
      541 gcgggtattt gtaaagcgga caaaaatctt cccaggcccg accagggaac tgtcttcggc
      601 agatacagca cctgcaacca tgatggccgt gacaacaagc acgaggatct tcatcgtacg
      661 cacggggttc ctcccttttt ccggtgaacg ccttaccgtt taaagcagcg gctgcccggt
      721 gaggggtttg ctcgtgagcg gcccagcgct gtggcccgcc ggcacggcgg ttgacagaac
      781 aggtattgtg tttcgcccgc tggattgcgc acaagggcgg cccagtgcgc gcacgccgtg
      841 ccacatctgc cggcattgcc atctcctcat cggaagtctc gggaaggctg ctcccatgat
      901 acaggtcgcg ggtgtccact gccataacgc ggtcagcagc gaaccagggc ttgcgatcac
      961 ggtggccgtt gatgcacagc ctctcgtcac ctttcagcat gctcgagcag atttgatttg
     1021 attccggaac ggcgcagtca ctaccatgct gtaaaccagg gtctgggagc atggcacgcg
     1081 ccgggggagc atggaaggta ttgcgaatga tcgacctgag caaattgaag aaccctctgg
     1141 attggataca ggcgcgcaag gacatcgaat cctcggtgct gaaggtgttg ggggcgcttc
     1201 caaaagagcg ggctgagctc cagatgaagg tcgtagacga agagtctttc ccgggataca
     1261 cgcggcgccg tgtgaactat tttgtgagcg actgggagcg catcgcggcg tggatgtttg
     1321 ttccgaaccg caaagagccg gtgccgggga ttgtatgctg ccacagtgaa catccggagg
     1381 ggaaatcaga gccagccggc ctggcggggg aacgcctgat ggcgctcgct gtgcattatg
     1441 ccgagcaggg ttacgcgacg ctcgcgcccg actgcgccac agcaggcgag cgggtgtccg
     1501 cgggcaaagc ggcctacgat acgtcaaact tttacaagga ttggcccaaa gggtcgttgc
     1561 tcgggaagat ggtgtgggac tatacgtgcg gtctgtacgc ccttggagat gtgcccggcg
     1621 tggacagctc ccgcctcggc atcatcggcc atggactggg cggcacctgc gccctgctgg
     1681 tggcggcgct tgacgagcgg gtgcaggtat gcgtggccag ctgcggtttt acccggttcc
     1741 acgacgacaa cgaggttggg cgttgggtga aggaagcgca ttgcgcactg gcgcccaacc
     1801 tgaaggacgc cgtcgcgaag aaggagtttc cgttcgactg ggaacatgtc ctggcgctgg
     1861 cagcccccac gcccacgctg ctgatcacgg cattgaatga cgcaaagctc ctcaatacca
     1921 agagttgcca gaaagcaaaa gatgccgccc ggcacatcta ccgcatgctg gggcgggatg
     1981 atgccctgga aaactacaca cattccgatg gccgcaccct gaccgtggac gccattgacg
     2041 ccgctgattc gtggttcgag cgttggctgt aatccggcag cctccgcccg gaggatgccg
     2101 gaaacggagg tcgcggaaaa cgcggttttc gggaatttgc cgaagaattg aatcgttgta
     2161 gccaaggtag gttggcgttc cctggctgga agggcggcga caccgggccg ccggggagct
     2221 gcttgatgcc gttcgctgca tgctcccacg agatgccggc gcggggcatg cgtacaggtc
     2281 tttgatccgt agaaacagtg gctctgcctg ccaaagccca gggagtagcg tttatgtcgc
     2341 aactgcgagt gatacgtaag gaatccgggc ataagacgag cggagaccaa atgccggctg
     2401 ttgacacgca ggtcgtggag cgcatcgcgg cgctggtgag cggtgtcgag cgtgtgattt
     2461 tcgggaagca cgacacggtg aaactgtgcg tgacgggact gctggcgcgc ggccatattc
     2521 tgattgaaga cgtgccgggc atcggcaaga ccacgctggc tcaggggttg gcccggtcga
     2581 tcgactgcaa attcagccgg attcaattta cgagcgacat gttgccctca gacattctgg
     2641 gggtaacggt gctgaatccg aagacccgtg agttcgaatt ccggccagga cccatttttg
     2701 ccggagtcgt cctggcggac gaaatcaacc ggaccccgcc gaaaaatcag agcgcgctgc
     2761 tggaagccat gagcgaatcc caggtctcgg tggatggagt gacccgcccc cttccccagc
     2821 cgtttgttgt cttggcgact cagaatccca ttgaatacga tggcacgtac accttgccgg
     2881 aatcgcagct cgaccggttc atgttgcgcg tcgaaatcgg gtatccgggg agtgatgacg
     2941 aattgcatat catgcgccgc cgggatccgc gtacagccat gacgcagctc gagccggtct
     3001 tatcggcagc ggaaatcgtg gcgcttcagg aacaggttcc tgacattcac gtggacaaca
     3061 gcgtggcccg gtacatgctg gcgatcgttc agggaacccg tcagcacgag cacattcagc
     3121 tgggggcaag cccgcgcgcg gcccaggagt tttacgaggc ctgccaggcg cacgcgctgg
     3181 tggagggccg taatttcgtg acgccggatg acgtcaaaca gctggccgta ccggcgctgt
     3241 cgcaccggat cctggtgcgt tcccgggaag gcaacctggc ggcggccgca cgggcgcgtg
     3301 cccgcgccgt atacgacgtg gtcaagaaca cgccaatccc acggtgagcg ccgtgaggac
     3361 gcgcaggcgc agcctgacgt ggcgcttgcc tcgagcgggc caggtattcg ttctgatgtc
     3421 ggccgtggtg ctcctggcgg cctggaacag cgggatgaac ctcctgtacc tgctggtggg
     3481 cgggctggtc agttttctgg tgctctccct ctttttttcc gcgaagaacc tgaaaggcct
     3541 gaaagttcag ttcgatgcgc cggccgcggt gcatcggggc gacaatgctg ccatcacggt
     3601 gcgcgtggag aatcacaagg cggtgcttcc gacggtgtcg ctgcgcattg aactcgccga
     3661 aaaccgcggt caatcggtgg cctatctggt caaactcccc gccggaaagg ccggagtcgc
     3721 gcgcatcaac gcgacgttcc cccgccgcgg cgtatatccg ccgcctccga tcgagctggt
     3781 gacgacattt cccttcgggc tgatagagac acggctccgg gtgcagggca ccacggaagt
     3841 gctggtgtat ccccgcgtgc attccgtgcg cccggccttt ctggacagcg tgcggggaac
     3901 gggtgaaatg ccgaggcgga tccgggggct gggcgacgaa tttttcggtc tgcgcggcta
     3961 tatgccgggc gacgatctgc ggcatgtggc atggcgcgtc agcgcacgcg cggacgagct
     4021 gatagtcaag gagatggcac acgaaacatc acggcacgtg cagctggtct ttgacgcgcg
     4081 ggaacgaacg gcccagccgg attttgagga acaattcgaa gaagcgatcg agctggccgc
     4141 atcactggga gagacgctgc tgagccggca gttccgggtg gggtttctga cggcgggcga
     4201 atacctgccg gcggacgagg gcaaggtcca gtcgacgcgt ttgctggaga tgctggcgcg
     4261 gctgcaaccg gaacccgggt cgctgccgga ccccttcttg caggcggtgc acgccgatga
     4321 ttcacgcgag gccacctgca ttttcgtgtc gcccgatccg gccgaatggg gcaggcgcat
     4381 cggggccggc cacgtgatgc gtccggaaga agcggtctat gcgtaggagt attgacagag
     4441 tcctgt
//
LOCUS       DAOPBQ010000299         4426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16274_length_4426_cov_4.043240, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000299 DAOPBQ010000000
VERSION     DAOPBQ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16274_length_4426_cov_4.043240"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKW60_09940"
     CDS             complement(<1..338)
                     /locus_tag="PKW60_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPJ99794.1"
                     /translation="MSQSTHHDRPHPEGQAGGFSLLDTVPCALIWQDVDGRIVECNRY
                     FAEQLLGQSLDSVVGKLPEELSDADHLQFLRRTESDASGAVLQPTQREVRFTGERGEI
                     RSFLATSAPVR"
     gene            complement(603..914)
                     /locus_tag="PKW60_09945"
     CDS             complement(603..914)
                     /locus_tag="PKW60_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HPJ99795.1"
                     /translation="MRVLNGADRKFLRGLGQKLEPTVMVGKNGVTATVIDSAGKALTA
                     HELVKVRFVEHKDEKRPLIDELARGTDSEVVGMIGHTALLYRQHDDPEKRKIHFPGGQ
                     P"
     gene            1102..2067
                     /locus_tag="PKW60_09950"
     CDS             1102..2067
                     /locus_tag="PKW60_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ99796.1"
                     /translation="MKKNFWIPAWSLAAALFMAACLAHAEDPHSFSIVLMPDTQNYSE
                     KYPETYLAQAQWIRDHAEAENIKFVIHVGDIVQNEHEENEWQLADAAHKLIDGHVPYS
                     VVPGNHDLRKEEKKVTRDTPFFDKYFPPERFQGQPWYGGHFGANNACNYCTFEAAGMK
                     FLVLSLSFAPDSAMLSWAGEVIQAHPEHRVIIATHYHMRPDGRATDEKPYGLDGYVGE
                     RLWENFIRKQPTVFMVVSGHVLGTHHQTATNDAGNPVHEILCDYQGEENGGNGWMQIM
                     RFVPADSTIRVEAFSPTLNQIRTELPDTYTLEYAMTEELAEAAAH"
     gene            complement(2103..3824)
                     /locus_tag="PKW60_09955"
     CDS             complement(2103..3824)
                     /locus_tag="PKW60_09955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPJ99797.1"
                     /translation="MTDDELEIRHPEAPANGEPVDPALLDTLLVRPRDKRSEAPQATF
                     PASTRYRLGQRIGQGGMAFVYEAYDTNLDRSVAVKSLRPEMAEDQSVRERFIYEALLL
                     GDMDHPGMVPIFEIGELDGAGLFYSMKRIKGRTLRQMLDSRPAKRFDRCEVAGLLSIF
                     GKVCQTVAYAHSRGIVHRDIKPENVMVDEYGVVLVLDWGLSKKIDEREVSQGITATQT
                     GIIKGTPAYMSPEQAEGRTHEIDFRTDVFSLGIILYEILCGHLPFPGKTRTEVLLQVA
                     QHQPPSPRRRQGSASPVLSAICMKALQKAPERRYSTARELSEDLERFSRGQETSAYRR
                     KFWERLFAWAGRRPAVATALAIFMSLGLFGSLGAIANHQYRREEAKLAMATGTRLART
                     MIGEMNRLDAEIASLRAQLDALSPGDSPERRLLEAQLAILKAGRQHQVAFVDRLGPVA
                     IARLAGAHIETVDEMGPDFANTAREIRLKEVESAFQVGELYEASHLVYKCILDAKRLA
                     WTPAQMSELLAWRDRIDGRLREALPAGAELPDWSRFDYDQVLRPFLDQIGVAGMDELR
                     KRTQNLR"
     gene            complement(3872..>4426)
                     /locus_tag="PKW60_09960"
     CDS             complement(3872..>4426)
                     /locus_tag="PKW60_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99798.1"
                     /translation="PEDTTFGVLVEGVSGHGGAALRIDVDGETVLSEEFADTDEGTET
                     LYVYDGTYRVPVSAGRHRIVVENPGIDWFYAAYEIPWLRAAPALRALGVAGDSQALVW
                     LQHRAHTWSNVAAGDFAESPVSGARALLRGLAEGTWRAEQWDTRTGRAEASEMHRVGD
                     SGQLELALPPIARDMAFRLYKVQD"
BASE COUNT          884 a         1430 c         1275 g          837 t
ORIGIN      
        1 cgcacgggcg cgctagtagc cagaaagctt cggatttcac cacgctcccc tgtaaagcgc
       61 acttcccgct gagtgggctg gagtactgcg ccggacgcat cgctttcggt gcggcgcaga
      121 aattgaaggt ggtccgcatc ggacagctct tccggcagct tgcccaccac actgtccaga
      181 gattgcccga ggagctgctc ggcaaagtac cggttgcact cgacaatgcg cccatccaca
      241 tcctgccaga tgagggcaca gggcacggta tccaacagac tgaaaccgcc ggcctggcct
      301 tccggatgcg ggcggtcatg gtgtgtggac tggctcatgc aattgctccg gaatatgcgg
      361 atccgcggcc cggcgcgata cacggccgct tctctctccc cacgcgcggg cacagcggcg
      421 gtattttgca ccggggctgc tgttggcgca cacgggcgcc gcccccgcgc tcaaaacgtc
      481 tcaggcgcgc gcctgccccg ccattgtagc agaagcatcc cgggcaggcg gtcccatttt
      541 tttggaggtt taacactgaa aaaggaatgc acttcacctg cccttcgtgc gcgaaaggcc
      601 tcttacggct gcccgccggg aaaatggatt ttgcgcttct ctggatcatc gtgctggcgg
      661 tacagcagag cggtgtggcc aatcataccg accacttcgc tgtctgtgcc ccgggcaagt
      721 tcgtcaatca gcggccgctt ctcgtccttg tgctccacga aacgcacctt cacaagctcg
      781 tgagcggtga gggctttgcc ggccgaatcg atcaccgtcg cagtgacccc gttcttgccg
      841 accattacgg tgggttcgag cttctgcccg agcccacgca ggaatttcct gtccgcgccg
      901 tttaatactc tcatgcatcc tccttgggag aggccgtgcc ggccgagcac aacctggcct
      961 ttgccacacg ccgctgctat tcgccccggc atgcgtggta tcttctcaaa cgaagatgat
     1021 agagtacgtg aacattcacc aacaaaccaa gatggcctgt tcgttcaggg ccggcacaat
     1081 gagcggtacg gagaggatac gatgaagaag aatttctgga ttcccgcctg gagcctggca
     1141 gcagcactgt tcatggcggc atgtctcgcg cacgccgaag atccccactc gttctcgatc
     1201 gtattgatgc ccgatacgca gaactattcg gagaaatacc cggagaccta cctggcccag
     1261 gcacagtgga ttcgcgacca tgcggaagcg gaaaacatca aattcgtgat ccacgtgggc
     1321 gacatcgtgc agaacgaaca cgaagagaac gaatggcagc tcgctgatgc cgcgcataaa
     1381 ctaatcgacg ggcacgtccc gtacagtgtg gtgcccggaa accacgacct gcgcaaggaa
     1441 gaaaaaaaag tcacccgcga cacgccgttt tttgacaaat actttcctcc ggagcgcttc
     1501 cagggccaac cctggtacgg cgggcatttt ggcgccaaca acgcgtgtaa ttactgcacc
     1561 ttcgaagccg ctgggatgaa gtttcttgtg ctcagcctgt cgtttgcgcc cgacagcgcc
     1621 atgctgtcct gggccggcga agtcattcag gcacacccgg aacaccgggt tatcatcgca
     1681 acccattacc acatgcgccc tgacggccgg gccactgacg aaaaaccgta cggactggac
     1741 ggatacgtcg gggaacggct ctgggagaat ttcattcgta aacagcccac agtattcatg
     1801 gtcgtgagcg ggcacgtgct cggcactcac catcagaccg caacaaacga cgcaggaaac
     1861 cccgtgcatg aaatcctctg cgattaccag ggcgaggaga acggggggaa cggatggatg
     1921 cagatcatgc gcttcgtgcc ggcggacagc acaatccgtg ttgaagcctt ctcccccacg
     1981 ctcaaccaga tccgcacgga gctgcccgat acctatacgc tggaatacgc catgacggag
     2041 gaactggcag aggcggctgc acactgaacc cctgcgagga accgtatcgt agacggcgct
     2101 cctcagcgga ggttctgggt gcgctttcga agctcatcca tgcccgcgac accgatctgg
     2161 tcgagaaacg gtctcagcac ctggtcataa tcgaaacggc tccagtccgg cagttctgca
     2221 ccggccggca gtgcctcacg caaacgtccg tcgatcctgt cccgccaggc aagcagctcg
     2281 ctcatctgag ccggggtcca cgccaggcgt ttcgcgtcga ggatgcattt gtagaccagg
     2341 tggctcgctt cgtaaagctc gccaacctgg aacgcgcttt cgacctcttt caggcggatc
     2401 tcgcgcgcgg tattggcgaa atccgggccc atttcgtcaa ccgtttcgat gtgggcgccg
     2461 gccagccgtg caatggctac cgggccgagg cggtcaacga atgcgacttg atgctgccgg
     2521 cccgccttca gaatggccaa ttgcgcttcg agcaatcgcc gttcgggtga atccccggga
     2581 gacagcgcat ccaattgagc gcgaagcgac gcgatctccg catcgagacg gttcatctcg
     2641 cctatcatcg tccgggcaag gcgggtgccg gttgccatgg caagcttcgc ctcttcgcgg
     2701 cggtattggt ggttggctat ggcgccaagg ctcccgaaca agcccagcga catgaagatg
     2761 gcgagcgccg tcgccacggc cggacgccgc cccgcccagg caaacagccg ctcccagaat
     2821 ttgcgccggt aggccgacgt ctcctggccg cggctgaagc gttcaaggtc ttcggaaagt
     2881 tcccgggccg tactgtatcg ccgctccggg gctttctgca gggctttcat gcagatcgcg
     2941 gagagtaccg ggctcgcgct tccctggcgc cggcggggcg atggcggctg gtgctgcgca
     3001 acctgcagta gcacttcggt gcgggttttc ccgggaaacg gcaggtgtcc acagagaatt
     3061 tcgtacaaaa ttatccccag cgaaaacaca tcggtacgaa agtcgatttc gtgagtgcgc
     3121 ccttcagcct gctccggact catgtaggcc ggcgtgcctt tgatgatccc ggtctgggtg
     3181 gcggtaatgc cctggctgac ctcccgttcg tcgatcttct tcgacaaccc ccagtcgagc
     3241 accagcacaa cgccgtactc atcaaccatg acgttttcag gcttgatgtc gcgatggacg
     3301 atgccgcgcg aatgggcata agccacggtc tggcacactt tcccgaagat ggagagcagc
     3361 ccggcaacct cgcaccggtc aaatcgcttc gccgggcgtg aatccagcat ctggcggagg
     3421 gtgcggcctt tgatgcgctt catcgaatag aaaaggccgg caccgtccag ttcaccaatc
     3481 tcaaatatcg gcaccatgcc cggatgatcc atgtcgccca gaagcagcgc ctcataaatg
     3541 aatcgttctc gcacagactg gtcttcggcc atctccggcc gcagggattt cacagccacg
     3601 gagcggtcca gattggtgtc gtaagcttca tagacaaagg ccatcccgcc ctggccgatg
     3661 cgctgcccca ggcggtaacg ggtacttgcc ggaaacgtgg cttgaggcgc ctcgctccgc
     3721 ttatcccgcg ggcgcaccag cagggtgtcc agcagcgcgg gatcgacggg ctccccgttc
     3781 gcgggcgcct ccggatgcct gatttcgagc tcatcgtctg tcatatcgtc agcatccctt
     3841 cctcatggat cgtctcgtcc cgcgcccgag cctaatcctg caccttatac agacgaaatg
     3901 ccatatcacg ggcgatcggg gggagcgcca gttcgagttg cccgctgtct ccgacacggt
     3961 gcatctccga agcctcagca cgaccagtgc gggtatccca ctgctccgca cgccacgtgc
     4021 cctccgccag gccacgcaga agcgcccgtg cacccgatac cggcgactcg gcaaagtcgc
     4081 ccgcagccac attgctccac gtatgtgcgc gatgctgcag ccacaccagc gcctggctgt
     4141 cgcctgccac ccccagagcg cgtaaagcgg gcgcggcgcg cagccagggt atttcgtatg
     4201 cagcgtaaaa ccagtcaatt cccggatttt ctacgacgat cctgtggcgg cctgccgaaa
     4261 ccggcacccg gtacgtgcca tcgtaaacat aaagggtttc ggtgccctca tccgtatccg
     4321 cgaactcctc cgaaagcacc gtttcgccat ccacgtcaat gcgcagcgca gcgccgccat
     4381 ggcccgacac gccttccaca agcaccccga atgtggtgtc ctcagg
//
LOCUS       DAOPBQ010000300         4417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16315_length_4417_cov_4.110271, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000300 DAOPBQ010000000
VERSION     DAOPBQ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16315_length_4417_cov_4.110271"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1467)
                     /gene="mnmE"
                     /locus_tag="PKW60_09965"
     CDS             complement(76..1467)
                     /gene="mnmE"
                     /locus_tag="PKW60_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPJ99799.1"
                     /translation="MAPPVTRTASEGTIAAISTPPGEGAIGIVRLSGPDALRIAAAVF
                     HSSRGLDIRHLRRRVYHGEIRNGAETLDEVLLHIMRAPHSYTREDVVEINAHGGAEPL
                     QAILELVLEQGARLAEPGEFTKRAFLNGRLDLVQAEAVIDRIRAKTRAGLRAANAAAS
                     GALSQAIAGMREPLLQALARIEASVDFPDEDLPELVTPELRATLAAIAARMETLLAAS
                     DAGRLYREGAAVIIAGRPNVGKSSLFNALLRDARAIVTARPGTTRDLLEEVITVQGIP
                     IRLSDTAGLRHSDDEVERIGVDVARSALNAADAVLFVVEATDPATPEDEALAGDLLAL
                     EVPVFLVVNKIDLNPSPALSSWADRFAATWLVSAKSGDGLQALEAALAKQLLGGAAMN
                     PSDVLLNRVHQRDSLRRARQALARLLDNFGASPEFLSIDLREALAALGEITGETTAED
                     VLDRIFASFCIGK"
     gene            complement(1436..2893)
                     /gene="jag"
                     /locus_tag="PKW60_09970"
     CDS             complement(1436..2893)
                     /gene="jag"
                     /locus_tag="PKW60_09970"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPJ99800.1"
                     /translation="MRSIEISARKREDAINKALSELGIERHEAHVEILDEGSPGFLGI
                     GVRDVTVRVSTDVPGEGGFPIEADGNRIDFEHQPMRNDRGHNQVRGARRGPKRDRRNG
                     DRDRGPRPPQHGRPAERAGHDRRDKNPRREQRPERPKNQRPEKPRDSRPKNDRPRNDR
                     PRHARPREDRPREERPRQERPRPEAVNRPPDKVLTDARSCEAAALLNEIIQGMGIESS
                     VQPLEATQGGLKLSVQSPDSALLIGRKGRNLTALQYLINRIFNRQDEWQQSERIVVDV
                     EGYLDRRRDTLEDMAQRLAERAKSSGRRVRVKPLSPQERRIIHLALHDDPDVRTFSVG
                     ESNFRSVIIAPVSEGDAGDEHDEGDDYDDANAFDMNGDDVLDDENGSGDEPRVRPETG
                     RSKAPESAAPSAVADDASPEVSSGDTVDEDEDGEGDEPDGDADKRFQPRRRVGRRGAV
                     RKRRPAKPLASVAANGEKSGAGETKDGTPSDADSV"
     gene            complement(2912..>4417)
                     /locus_tag="PKW60_09975"
     CDS             complement(2912..>4417)
                     /locus_tag="PKW60_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HPJ99801.1"
                     /translation="FAAQQELGDRLNYRRFDAIIDPSGQAVTFTLASPVLAVTKSFAL
                     TDTPFLVDASVRIENREDAGRRFGLDQTPAYLFAWAPDVDSPDKTKGVGQTIVIRSEN
                     HNEYQDTRKLKAGNPGWTVPGADWIAVKGAYFVVALKHLAGETIGAQSGNAFYRGVAG
                     DLTAGITVPRMEIAANSADTQAFRVYIGPSRLDSLAAAWPTLDTAPRFFLSDTWAFMD
                     KFAKFLLRLLNLFYSWIPNYGIAIILLTVLVRLAMLPLTVKSMKSMKKMQQLAPEIEK
                     LREKYKEDQQELNKRMMEMYRERGVNPVGGCLPLFLQMPIFIALYRMLWSAYELRGAP
                     FMLWITDLSQPDHLFHLPNLTALPMVGQHLEYFNLLPILMALAMLVSQKVLPQPGAAA
                     NPQQKTMMTIMPVFFAFVCYRVASGLNLYILVSTLLGIVQQTFTRMQKDVDVTPDKLK
                     APRKRQHFYKAAQERKRRIAKEAEAAARKSRATRVTGAEKEPKNAPRTDER"
BASE COUNT          733 a         1326 c         1489 g          869 t
ORIGIN      
        1 gccacgctcg aactctgaga aatcccgccg catctccctg acgcgcccgc actcccgttt
       61 cgtgctgggc tgccgctatt tcccgataca aaacgacgcg aaaatgcgat ccagaacatc
      121 ttcggcggta gtctctccgg tgatttcgcc gagcgcggcg agggcctcgc ggaggtcgat
      181 gctgaggaat tcgggcgacg cgccgaaatt gtcgagcagg cgggcgagag cctggcgggc
      241 gcgccggagg ctgtcgcgct gatgcacgcg gttgagaagc acatcggacg gattcatggc
      301 cgctccaccg agcagttgtt ttgccagcgc ggcctcgagc gcctgaagcc catcgccgga
      361 cttcgcggat acgagccagg ttgcggcaaa acgatccgcc cagctgctga gggccggcga
      421 agggttcagg tcgattttgt tgaccaccag aaacacgggg acctcgaggg ccagcaggtc
      481 gccggccaaa gcctcgtcct ccggagtggc gggatccgtg gcttccacca caaaaagcac
      541 cgcgtcagcg gcattcaggg cactgcgggc gacgtcgaca ccgatccgct cgacttcgtc
      601 gtcggaatgg cgcagaccgg cggtatcgct caggcggatc gggatgcctt gcaccgtgat
      661 gacttcttcc aggagatcgc gagtcgtgcc gggccgggcg gtgacaatag cgcgggcgtc
      721 acgcaacaat gcgttaaata gactggattt accgacgttg ggccgccctg cgatgatcac
      781 ggccgcgccc tcgcggtaga ggcggccggc atccgaggca gccaggagcg tctccatgcg
      841 ggcggcgatg gcggccagag tggctcgaag ttccggcgta acgagttcgg gcagatcttc
      901 gtcgggaaaa tccaccgagg cctcgattcg cgcgagggct tgcaggagcg gttcgcgcat
      961 gccagcgatg gcctgagaga gggcgcccga ggcggcggca ttggcggcgc ggagtccggc
     1021 gcgggttttt gcgcggatcc ggtcgattac cgcttcagcc tgcaccagat cgagccggcc
     1081 attgaggaag gcccgtttgg tgaactcgcc gggctcggcc aggcgcgcgc cttgctcgag
     1141 aacgagttca aggatagcct gaagcggctc cgcgcccccg tgagcattga tctcgacgac
     1201 gtcctcgcgg gtatagctgt gcggggcgcg catgatgtgc agcagtactt catcgagggt
     1261 ctcggcgccg ttgcgtatct ctccgtgata gacgcgcctg cgcaaatggc gaatatcgag
     1321 ccctctggaa gaatggaaaa cagcggccgc gatgcggagc gcatcggggc cgctgagtcg
     1381 cacaatacct atggcgcctt caccgggcgg ggtggaaatc gccgcgatgg tgccttcaga
     1441 cgctgtccgc gtcactgggg gtgccatctt tggtttcccc ggcgcccgat ttctcgccgt
     1501 ttgcggcaac ggaagccagg ggtttagcag gccggcgttt gcgcacggcg ccgcggcggc
     1561 cgactctccg ccggggctgg aagcgtttgt ccgcatcccc gtcgggctca tcgccctcgc
     1621 cgtcttcgtc ctcgtcgacc gtgtcgccgc tgctgacttc gggagacgca tcgtcggcca
     1681 cagcagaagg ggcagcgctt tccggcgctt tggagcgccc cgtttcaggc cgaaccctgg
     1741 gctcatcgcc gctgccgttt tcgtcgtcca gcacgtcgtc gccgttcata tcaaatgcat
     1801 tcgcatcgtc gtagtcatcg ccttcatcgt gctcgtcgcc ggcatcccct tcggagaccg
     1861 gcgcgataat gacgctgcgg aaattggatt cgcccacgct gaaagtgcgc acatcgggat
     1921 cgtcatgaag ggcgaggtgg atgatgcggc gttcctgcgg gctgagcggc ttgacgcgca
     1981 cccgccgtcc ggacgatttg gcgcgttcgg ccaggcgctg ggccatatct tccagcgtat
     2041 cgcgcctgcg gtcgagatag ccttccacgt cgaccacaat gcgttcggac tgctgccatt
     2101 catcctggcg gttgaaaatg cggttgatga ggtactgcaa ggccgtcagg ttgcggcctt
     2161 tacgcccaat caacagggcg gaatcgggag attggaccga gagcttgagc ccgccctgag
     2221 ttgcttcgag aggctgtacg ctggattcga tccccatgcc ctggatgatc tcgttgagaa
     2281 gcgcggccgc ctcgcagctg cgcgcatcgg taagcacttt gtccgggggc cggttcaccg
     2341 cctcaggccg cggccgctcc tggcgtgggc gctcttcacg cgggcgatct tcgcgggggc
     2401 gggcatggcg gggccggtcg ttgcggggcc ggtcgttttt ggggcggctg tcgcgaggct
     2461 tctcgggacg ctggtttttc ggccgctcgg gacgctgctc gcgccgcggg tttttgtcgc
     2521 gccggtcgtg gcccgcccgc tcggcgggac gtccatgctg cggcgggcgg ggcccccggt
     2581 cgcggtcgcc gtttctccga tcgcgtttcg ggccacgccg ggcgccgcgg acctggttgt
     2641 gtccgcggtc attgcgcatg ggctgatgct caaaatcgat ccggttgcca tcggcttcga
     2701 tggggaatcc gccttcgccg gggacatcgg tggatacgcg cacggtgacg tcgcggacgc
     2761 cgatgccgag gaatccgggg ctgccctcgt ccaggatctc gacatgcgct tcatggcgtt
     2821 cgatgccgag ttcgctgagt gctttgttga tagcgtcttc ccgtttccgg gcactgattt
     2881 cgatggatct catggaatga actttcctcc tctaccgttc gtccgtccgg ggcgcgtttt
     2941 tgggttcttt ttcggcgccc gtgacacggg ttgcgcggct cttgcgtgcc gcggcctcgg
     3001 cttctttggc gatgcggcgc ttgcgttcct gcgcggcctt gtagaaatgt tggcgcttac
     3061 gcggagcctt gagcttgtct ggggtcacat cgacgtcttt ctgcatgcgg gtgaaagtct
     3121 gctgcacaat gccaagcagg gtgctgacca ggatgtagag gttcaggccg gacgcgacgc
     3181 ggtagcagac gaacgcgaag aagaccggca tgatggtcat catggttttc tgctgaggat
     3241 tggcggcggc gccgggttgc ggcaacacct tctggctgac cagcatggcc agggccatga
     3301 ggatcggcag caggttgaag tattcgagat gctggcccac catcggcagc gcggtcaaat
     3361 tcgggagatg aaagagatgg tccggctggg acaggtccgt gatccacagc atgaacggtg
     3421 cgccacggag ttcgtacgcg ctccacaaca tccggtagag cgcgatgaaa atgggcatct
     3481 gcaggaatag cggcaggcaa ccacccacgg gattcacgcc gcgttcccga tacatctcca
     3541 tcatgcgttt gttgagctct tgctggtcct ccttgtattt ttcacgaagt ttttcaattt
     3601 ccggggcaag ctgctgcatt ttcttcatgc tcttcatgct cttgaccgtc agcggcagca
     3661 tcgcaagacg cacaagcacg gtgagcagga tgatggcaat gccgtagttt ggaatccacg
     3721 aataaaacag gttgagcagg cgcagcagaa acttggcgaa cttgtccata aaggcccagg
     3781 tatcgctcag aaagaaccga ggcgcggtgt cgagcgtggg ccaggccgcc gcaagggagt
     3841 cgagccggct cgggccgatg tacacacgga acgcctgtgt atcggcggaa ttggcagcaa
     3901 tttccatgcg cggaacggtg atgcccgcgg tgaggtcgcc cgcgacgcct cggtaaaatg
     3961 cgttgccgga ctgagcgcca atagtctccc cggccaggtg cttcagcgcc accacaaaat
     4021 aggcgccctt gaccgcgatc cagtcggcgc cgggcaccgt ccaccctgga ttgcctgcct
     4081 tgagcttccg ggtatcctgg tactcgttgt gattctcact gcggataaca atggtctggc
     4141 cgacgccctt ggtcttgtcg ggggagtcga catcgggagc ccacgcgaaa aggtaggccg
     4201 gggtctggtc caggccgaac cggcggccgg cgtcctcccg gttttcgatg cgtacactgg
     4261 cgtccacgag aaatggcgtg tcggtgagcg caaatgattt ggtgacggcc agcaccgggg
     4321 aggccagggt aaaggtcacc gcctgaccgg acggatcgat gatcgcgtca aagcggcggt
     4381 aattgaggcg atcgccaagc tcctgctggg cggcgaa
//
LOCUS       DAOPBQ010000301         4377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16491_length_4377_cov_3.367654, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000301 DAOPBQ010000000
VERSION     DAOPBQ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16491_length_4377_cov_3.367654"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..903)
                     /locus_tag="PKW60_09980"
     CDS             complement(175..903)
                     /locus_tag="PKW60_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPJ99802.1"
                     /translation="MIDRFSGAGFSGTTKVEVHAHTSKYSVCSHIPPRELIAMADASG
                     YDALFLTEHDRVWSTSEIAGLQELAEHVRVFPGIEIGLTDAIHVLVLGAQDPVYERLK
                     TPSDVFAQACVDGFLTVVAHPFRWSDKLPEYCRLADAVEVRTCNHGLEDHVTAARAYA
                     RAQNMAEIYASDAHGLNFMNKFWLETSEPFGSPQEFRHLILAGRYTNHAREFEMPLPP
                     PGKVASMSDLTEEDQLALWVQPTL"
     gene            1528..2694
                     /locus_tag="PKW60_09985"
     CDS             1528..2694
                     /locus_tag="PKW60_09985"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HPJ99803.1"
                     /translation="MKSERGIAILTIEDEPMVRRTLAAYLRKSGFTVHEAGDGAEGLA
                     LFERERPDLVLVDLRMPGMDGFAVLEALSAQSPQTPLVVVSAADAIDDAIEAQRKGAW
                     DYVLKPISPLSALGLTVEKVLERAELLRQNERYHRRIEETLRKIREDEKAGRHVQMRL
                     LPPEQWCFGPYILSHKLMSSMELSGDFVDYFAIDDEHLGFYTADVSGHGVSSALVTVI
                     VKTLIRKYHEQHLNQLDDAILNPGALLERLNSDLLNEDLEKHVSMFYGVLNRSKNLLR
                     FSNGGQFPSPFLWSAQGVEVLREQGAAVGLFPFARYGTYEKRLPARFLLAIFSDGVLD
                     ILPQPTLQHKLAFLRSLGSDSGIGRFMSVVQTNAQPPDDVTILSIKREGAQHGD"
     gene            2684..3187
                     /locus_tag="PKW60_09990"
     CDS             2684..3187
                     /locus_tag="PKW60_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPJ99804.1"
                     /translation="METKIRYAHAEHWCVLQLSGDIRHPVSSVVEAIIDEFLQAGDAS
                     EVLIDLTGAAFIDSTNLGLLAKVARDSWRRGQDPPILVSTNPDINAVLESMGFSSAFH
                     IVSEHEASALELHDVPTPDDYSDPRKAQRILEAHEALIELDEKNRQTFQSVVDVLRRN
                     NAPDGPP"
     gene            3833..>4377
                     /locus_tag="PKW60_09995"
     CDS             3833..>4377
                     /locus_tag="PKW60_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPJ99805.1"
                     /translation="MGNTTPPPRHARFWLKSAGAALVLLLVFGLGYMLRGSGTPPGAE
                     PDLQHSANTPVTQERIWTCSMHPQIRQNKPGLCPICGMDLVPVAASDNAAAGPREFVA
                     SEGAKALMNVETAEVLRRFVTREIRMVGKIDYDETRLAYITAWVPGRLDRLYVDYTGI
                     RVNKGDHMVRLYSPELLVAQD"
BASE COUNT          848 a         1387 c         1340 g          802 t
ORIGIN      
        1 cggggtacgg tgcgggcctt ccggatcatc gatgaggcgc tggtgacggg gcacgaagga
       61 gacgcgcaca aaaaaagcgc cgctggagat accgcgcccc gggccgtgga gaatcccgaa
      121 cgccccgccg atgcgagggc gccggccggc tcgccgggcg aataggccgg cgggctacag
      181 cgtgggctgc acccagagcg cgagctggtc ttcttcggtc aggtcgctca tgctggccac
      241 tttcccgggc ggcggcagcg gcatctcaaa ctcgcgcgca tggttggtgt agcgtcccgc
      301 cagaatcagg tggcggaact cctggggaga gccaaacggc tcgcttgtct caagccagaa
      361 cttgttcatg aaattcaggc cgtgggcgtc agatgcgtaa atttcggcca tgttctgtgc
      421 ccgcgcgtac gcccgggctg ccgtcacatg gtcctcgagc ccgtggttgc aggtgcgaac
      481 ctcgactgcg tcggccagcc ggcaatactc cggcagcttg tcagaccagc ggaacggatg
      541 tgccaccacg gtcaaaaatc cgtccacgca ggcctgggca aatacatcgc tcggcgtctt
      601 gagccgctcg tacaccggat cctgcgcacc cagcaccagg acatggatcg cgtccgtcag
      661 tccgatctca atgcccggaa atacgcggac atgctccgcc agctcctgca gccccgcgat
      721 ttcagacgtg gaccagaccc ggtcgtgttc cgtcaggaac agagcgtcgt agccgctggc
      781 gtccgccatc gcgatcaatt cccggggagg gatatgcgag cacaccgaat acttcgaggt
      841 atgggcatgc acctccacct tcgtcgtacc ggagaaacct gcaccggaga atcggtctat
      901 catggctgat ccattctatg cgggaattcg gtcagcaccc gccgtttcgt cacctgaagt
      961 ataccatgaa ttcaggctgc ctttccgtgc gtcgccgggt tccctgcctg cgccgttgcg
     1021 ctgccacgcg gggacaactg gttacaacct gttctcccat catcccggta acacgggatc
     1081 gaagtcacgg attcccaaca ggttcacccg gaagaccgcc aatgcgttcg cggaagcggc
     1141 gtgctgccgg gtgcacagga aaccggacgc agcagcctgc agaacattca atccccggcg
     1201 gcaacttctc gttccaggga agattcgcaa gcacggcatc cgtcgcgtgg acggcatgat
     1261 gaaagtatga aaaacaacga aaattcccta tagtaacggt cgcgcaacgt tacgtttggt
     1321 gcagtttctg aaacccctgc ggcggcgatg ggtttgaaag gcgtagcgga ttctgactga
     1381 attgactgga acaggtatac tgaagcctgg gctgctggaa cctcagggcg gaagactgcg
     1441 ctgctcccaa aggtttcacc agggactgcc gtgattggcg ctgcggcatg tggagcttca
     1501 tgtatcaacc tccaggagca tgcggccatg aaatcggaga gaggcatcgc catcctcacc
     1561 atcgaggacg aacccatggt gcgccggacc ctggccgcct acctgcgcaa atcggggttc
     1621 acggttcacg aagcgggcga tggtgctgaa gggcttgcgt tgtttgagcg tgagcgcccg
     1681 gatctcgtcc tggtcgatct gcgaatgccg gggatggacg gatttgccgt gctcgaggcc
     1741 ctcagtgcgc aatccccaca gacccccctg gtggtggtgt ctgccgccga cgcgatcgac
     1801 gacgccatcg aggcccagcg caagggcgcg tgggattacg tcttgaaacc gatcagcccg
     1861 ctgagcgcgc tcggcctgac cgtggaaaaa gtgcttgaac gggccgaact gctccggcaa
     1921 aacgaacggt accaccgccg gatcgaagaa acactccgga aaatccggga agacgaaaaa
     1981 gcaggccggc acgtccagat gcggctgctt ccgccggagc aatggtgttt cggcccctat
     2041 atcctgtcgc acaaattgat gtcgtcgatg gagctcagcg gcgatttcgt tgactatttt
     2101 gccattgacg acgagcatct cgggttctac acggccgacg tatccgggca cggggtgtcg
     2161 tcggcgctgg tgaccgtgat cgtcaagacg cttattcgaa aataccatga acagcatctc
     2221 aaccagctcg acgatgcgat cctgaatccc ggcgccttgc ttgagcgcct gaacagcgac
     2281 ttgctcaacg aagatctcga aaagcacgtg tcgatgtttt acggtgtgct gaatcgttcg
     2341 aagaatctgc tccggttctc gaatggcggg caattcccct cgccgtttct ctggtctgcc
     2401 cagggagtcg aggtcctccg ggagcagggc gcggccgtag gactgtttcc ctttgcccgc
     2461 tacggaactt acgaaaaacg tctgcccgcc cgctttctcc tggcgatatt ctccgatggg
     2521 gtgctggata tcctgcccca gcctacgctg cagcacaagc tcgcgtttct gcgctcgctg
     2581 ggaagcgatt cgggcatcgg ccggttcatg tcggtcgtcc agaccaacgc ccagccgcct
     2641 gatgacgtga ccatcctgag catcaaacgg gaaggagcgc agcatggaga ctaaaatccg
     2701 ctatgcacac gcggagcact ggtgcgtcct ccagctctcg ggcgatatcc gccacccggt
     2761 cagctcggtc gtcgaggcga tcatcgacga atttctccag gccggggacg cctccgaagt
     2821 gctcattgac ctgacgggcg cggcctttat cgacagcacc aacctcggcc tgctggcgaa
     2881 agtcgcccgc gactcctggc ggcgcggcca ggatccgccc atcctcgtat cgaccaaccc
     2941 cgacatcaac gccgttctcg aaagcatggg gttttcgagc gcgtttcaca tcgtttcgga
     3001 acatgaagcg tccgctctgg aactgcacga tgtgcccacc ccggacgact attccgatcc
     3061 gcgcaaggcc cagcgcattc tcgaggccca tgaagcgctc atcgagctcg atgagaaaaa
     3121 ccggcagacc ttccagagtg tcgtggatgt actgcgccga aacaatgcgc ctgacgggcc
     3181 tccgtagccc cggggcacac gctcgcttgt ccgtggcggc ggcggggtga acggaatagg
     3241 cacttcatgc ggggtgttga gtccagacag gcaagaacaa gtcgatgcgt aacgtaaaaa
     3301 cacctcgaag actggcggcg cttgtcgctg cggtggccct gacgttcgcg ctgggccccg
     3361 cgggcactgc gtgtctgtgc ggcgcgggcg aacatgccgc atgcgcagca cccggggccg
     3421 gccacaccgc ggagccttcc cctgccagta cctgttgtgg cgcggaacgc ccggaaccag
     3481 ccggttccgg gccggtggat tccgcggggg ccgttatgca tcgttgtgtt tgtgcgaccc
     3541 atggaccgca ggccgggacg gccatcctgg cggcctccac ggtgcagtcc actgccaagc
     3601 cgtgcgcctc tactcttacc gtggatgccc cgccggcgcc gcaccccagg cacacgcaaa
     3661 aggattttgc cggcgcaacc gcgcttctcc cggcggccgc caatccacca ccttcatttc
     3721 tcatagcctg cgcactcctg atgtagcgtg agccctgccg agccggcacg gttgactaac
     3781 gcattgaaat tcgcggcctc gtaacacagg ccggtgcagg agtgtcgaga tcatgggaaa
     3841 taccacacca ccaccccggc acgcccgttt ctggctgaaa tcagcgggcg cggcgcttgt
     3901 gctgctcctg gtgttcgggc tgggctatat gcttcggggg tccggaacgc ctccaggcgc
     3961 cgaaccggac ctgcagcaca gcgcaaatac accggtcacg caggagcgca tctggacctg
     4021 ctcgatgcac ccccagatcc ggcagaacaa gcccggcctg tgtccaatct gcgggatgga
     4081 cctcgtcccc gtcgctgcct cagacaacgc cgcagcgggt ccgcgcgaat ttgttgccag
     4141 cgagggcgcc aaggcgctca tgaatgtcga aacagcggag gtgctgcggc ggtttgtgac
     4201 gcgggaaatc cgcatggtcg ggaagatcga ttacgatgag acgcgcctcg cctacatcac
     4261 cgcctgggtt cccggccggc tcgatcgcct gtacgtggac tacacgggca tccgcgtcaa
     4321 caaaggcgat cacatggtgc gcctctacag ccctgaactg ctcgtggcgc aggacga
//
LOCUS       DAOPBQ010000302         4364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16550_length_4364_cov_3.104897, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000302 DAOPBQ010000000
VERSION     DAOPBQ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16550_length_4364_cov_3.104897"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="PKW60_10000"
     CDS             <1..1536
                     /locus_tag="PKW60_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99806.1"
                     /translation="GKRQLWANGIEQPLWSPNSDEFWDWTEKYIIEYAKMSAENRHIL
                     GVFLDYENYAPGRTGDLYSLSYDDIILQKFAQAQGIDLPELDRAARKPWLDEQGLHDA
                     FEAFQIDHWRERCRHLRQRVDEYDPTFQFCIYPAPGTPFMLQAACPEWATERAPIILA
                     DASTYGRPARYLPERDSLEGNRRTLLKRMQKAKDAAIPYIYAGGIDPVVRGADPEFSG
                     KNAVAITSVTDGYWIFYEGPKYKVDHPDYWKWFSWANARIAAGELDAWKQPRETPEKF
                     ALDMFGDKPAVPGLEVPEFTGEAVDNPGIVLRGDNLLAIACKKGVPVEVVLKHHPISD
                     YQAVLAWDLRSLAMDALQSGTVGMKQSGTIQFTPEEDAICLLGLSAGGCAYSIPRANV
                     PIGLLTGDGASFIREAKPLYFFVPESVETFTVSARGGGGAETVRINVLAPDGTRAATG
                     QTTLKEGKVTITVDTSSNAGQVWSLEAVRAEEGVIEDYSVTLGDTLPPVLAFHREHVF
                     RRK"
     gene            1639..2379
                     /locus_tag="PKW60_10005"
     CDS             1639..2379
                     /locus_tag="PKW60_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015847134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HPJ99807.1"
                     /translation="MNDGLRITVWNEGVHDKRDQKVMELYPDGMGNAIARYLSEQPGI
                     ASVRVSQLDDPGQGLTDEILASTDVMTWWGHCAHDQVTDENAAKVKQRVLEGMGLVVL
                     HSGHFSKAFKSLMGTGCDLKWREIGEKERLWVVDPAHPIAEGLSEYFELPHTEMYGEY
                     FDIPQPDQLVFISWFEGGEVFRSGCCWHRGLGKVFYFRPGHETLPIYHDQNVLKVIYN
                     GVRWAAPSGLKSVVRGNVQPLENIGGSK"
     gene            2593..3636
                     /locus_tag="PKW60_10010"
     CDS             2593..3636
                     /locus_tag="PKW60_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPJ99808.1"
                     /translation="MGLFSSQISAKKMVPLCRELATSYDAGIPIIRSLEIIGGQQQDR
                     RIRAIFTEMAGAVKAGNTLDQAAREQHKYLPAFFTELIASGEVGGKLDVMLRDLADYY
                     EDRLERQRRVVRMMALPVLELIAAWFLGTFALRLVGRIIGALSGAGGGNFDFEAFLKD
                     YALFQIKALGVFAVLFVGVVILSRMGLFGWVSSLFTTHIYPLSAVTKRFALARFFRSL
                     SLLIGAGVHVPVAIERSAAVTANPYIERDLLKTIPPVKQGSTLVEAFQRSRCLTPTAR
                     EMLLVGEESGKLDETLRKVADYHFEEANQAVDIAAKMFGVLLVLAVALTIGYIIVTFY
                     MKFYGGMMDELGI"
     gene            3620..4147
                     /locus_tag="PKW60_10015"
     CDS             3620..4147
                     /locus_tag="PKW60_10015"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPJ99809.1"
                     /translation="MSSGFNEPDFDSAAEVKDRTLWWWATVGILVVAFLGALAIWGRS
                     DPNTSRVRARHILISFEQNDPADRARALERAQDLRQRIINGANFSKLAKEYSNDSFSA
                     NRGGDLNYQKKGSLEQAVEDFVWTAPIGQVSDIIQTKYGFHIVRVEDRYLSEADKYDL
                     EMKQRAMGGETGRGQ"
BASE COUNT          874 a         1377 c         1309 g          804 t
ORIGIN      
        1 ggaaaacggc agctctgggc caacggcatc gaacaacccc tctggagccc caattcggac
       61 gaattttggg actggaccga aaaatacatc attgaatacg cgaaaatgag cgccgaaaac
      121 cggcacattc tcggcgtctt tctggattat gaaaactatg cccccggccg aaccggcgat
      181 ctgtacagcc tctcgtacga tgacatcatc ctgcagaaat ttgcccaggc ccagggaatc
      241 gacctgcccg aactcgaccg ggccgcgcgc aaaccctggc tcgacgaaca gggcctccac
      301 gacgcattcg aggcgtttca gatcgaccac tggcgcgaac ggtgccgcca tctgcggcag
      361 cgcgtcgacg agtacgaccc cactttccag ttctgcatct atcccgcgcc cggcacgccg
      421 ttcatgctgc aggccgcctg ccctgaatgg gccacagagc gcgcccccat catcctcgcg
      481 gatgcctcaa cctacggccg ccccgcgcgt taccttcccg aacgggattc tctcgagggc
      541 aaccgccgaa cccttctcaa acggatgcag aaagccaaag acgctgcgat cccctacatc
      601 tacgcgggcg gcatcgaccc ggttgtccgg ggtgcggacc ccgagttcag cggcaagaat
      661 gccgtcgcca tcaccagcgt gacagacggc tactggatct tctacgaagg ccccaaatac
      721 aaagttgatc atcccgacta ctggaaatgg ttctcctggg ccaacgcgcg tattgccgcg
      781 ggcgaacttg acgcctggaa acagccccgc gaaacgcccg agaagttcgc gctcgacatg
      841 ttcggcgaca aacccgccgt gccgggcctg gaagtgccgg aattcaccgg cgaggccgtc
      901 gacaaccccg gcatcgtcct gcgcggcgac aacctcctcg ccatcgcctg taaaaaaggc
      961 gtcccggtcg aggtcgtgct caaacatcat cccatttccg actaccaggc ggtactcgcc
     1021 tgggatctcc gttccctcgc catggacgcg ctccagtctg gaacggtggg catgaagcag
     1081 tccggcacga ttcagtttac gccggaagaa gacgccattt gcctcctcgg cctctcggca
     1141 ggcgggtgcg cctacagcat cccgcgggcc aatgtgccca tcggactgtt gaccggcgac
     1201 ggtgcttcgt tcatccgtga ggccaagccg ctctatttct ttgtgccgga gtcggttgag
     1261 acattcacgg tctccgccag gggtggcggc ggcgccgaga ctgtccgtat caatgtactt
     1321 gcaccggacg gcacccgggc cgcaaccggc cagacgaccc tgaaagaggg aaaggtcacc
     1381 atcacggtcg ataccagcag caacgcgggc caggtgtggt cgctggaggc cgttcgcgcc
     1441 gaagaaggcg tcatcgaaga ctattcagtg acgctcggcg ataccttgcc gccggtcctg
     1501 gcatttcatc gggaacacgt gttccggcga aagtaaagag cgtggcgagg gccggttgtc
     1561 cgcggattgc tttacctgca tctcccgtgc ggttaaaatg ctgcccggcg gcgagaagca
     1621 ctgacgaaag gatgggccat gaacgacgga ctccgcatca cggtatggaa cgagggggta
     1681 cacgataaac gcgaccagaa ggtcatggag ctctatcccg acgggatggg caacgccatt
     1741 gcccggtacc tctcagaaca gcccggcatc gcctctgtgc gtgtgtctca gcttgacgat
     1801 cccggtcagg ggctcacaga cgaaatcctg gccagtaccg acgtcatgac ctggtggggc
     1861 cattgcgccc atgaccaggt gaccgatgag aatgcggcga aggtcaagca gcgtgttctg
     1921 gaaggcatgg ggctggtcgt cctgcattcc ggccacttct ccaaggcgtt taagagtctg
     1981 atgggtaccg gttgcgacct caaatggcgc gagatcggcg agaaggagcg cctttgggtg
     2041 gtcgacccgg cccatcccat tgccgagggc ctgtccgagt atttcgaact gccccacacc
     2101 gaaatgtacg gcgagtactt tgacatcccc cagcccgatc agctcgtgtt tatcagctgg
     2161 tttgaaggcg gcgaggtgtt tcgcagcggg tgctgctggc atcgcggcct gggcaaggtc
     2221 ttctacttcc gccccgggca cgaaaccctg ccgatatacc acgaccagaa tgtgctcaag
     2281 gtcatttaca acggcgtccg gtgggcggcc ccgtccggac tcaaaagcgt cgtccggggc
     2341 aacgtgcagc ctctggaaaa catcggcggg agcaagtgaa acccggggag ggctcctgaa
     2401 ccgccgggcc cggcgtgaac gggcgcgagc cggttcgcca ggctggaaat ggccatgcgg
     2461 gcgccggcag cggcgcgctt ccccggcaga aacccggcca ccctcccgcc ggcccgcaca
     2521 ctagcgcagc gtgcggtcgt ttgctacaat cgggcgtggt gcgccaggcg cgcgccaacg
     2581 ctggagtgcc gtatgggtct gttttcctct caaatatcgg ccaagaaaat ggttccgctg
     2641 tgccgggaac tcgcgacgtc gtacgacgcg ggcattccca tcattcgttc gctcgaaatc
     2701 atcggcgggc aacaacagga ccggcggatc cgcgccattt tcaccgagat ggcaggcgcc
     2761 gtgaaggccg gaaacaccct ggaccaggcc gcgcgcgaac agcacaaata cttgcccgcg
     2821 tttttcacgg agcttattgc cagtggcgag gtcgggggca agctcgacgt gatgctgcgc
     2881 gatctggccg actactacga agaccggctc gagcgccagc gcagggtcgt gcgcatgatg
     2941 gccctgcccg tgctcgagct gatcgccgcc tggttcctgg gcacgttcgc cctgcgcctg
     3001 gtggggcgga tcatcggggc gctatccggt gcaggtggcg gaaatttcga tttcgaggcg
     3061 tttttaaaag actatgccct gttccagata aaggcgctgg gcgtatttgc cgtgctcttt
     3121 gtgggcgtcg tgattctctc gcgcatgggc cttttcggct gggtttcgag cctgtttacg
     3181 acccacatct acccgctgtc cgccgtcacc aaacgctttg ccctggctcg gtttttccgc
     3241 agtctgtcac tgctgatcgg cgccggcgtg cacgtacccg tggccattga acgctccgca
     3301 gccgtcaccg cgaacccata tatcgagcgc gacctgctca aaaccattcc accggtaaag
     3361 cagggcagca ccctcgtcga agcatttcag cgctcccgct gcctgacacc caccgcgcgc
     3421 gaaatgctgc tcgtgggtga ggagtccggc aaacttgacg agacgcttcg caaagtggcc
     3481 gactaccatt tcgaagaagc caaccaggcg gtcgacattg ccgcaaaaat gttcggcgtg
     3541 ctgcttgtcc tggctgttgc tctcaccatt gggtatatca ttgtcacgtt ctacatgaag
     3601 ttctatggag gcatgatgga tgagctcggg atttaatgaa ccggatttcg attccgcggc
     3661 cgaagtcaaa gaccgcacgc tctggtggtg ggccacggtg ggcatcctcg tggtcgcgtt
     3721 tctgggggcg ctcgccatct ggggccgaag cgatcccaat acgtcgcgcg tgcgcgcgcg
     3781 ccatattttg atctcgtttg agcaaaacga ccccgcggac cgcgcccgcg ccctcgaacg
     3841 ggcccaggat ctgcgccaac gcattatcaa tggcgcaaac ttcagcaaac tggcgaaaga
     3901 gtattccaac gacagtttca gcgccaaccg cggcggcgac ctcaactacc agaaaaaggg
     3961 gtcgctcgaa caggcggtgg aagatttcgt ctggaccgct cccatcggac aggtcagcga
     4021 catcatccag accaaatacg gctttcacat cgtgcgggtc gaagatcgct acctctccga
     4081 ggccgacaag tacgacctcg agatgaaaca gcgcgccatg ggaggggaaa cgggcagggg
     4141 gcaatgaccg gccctgtcgg aatacacgac gccgcgccgt ccgcaagtcc tgctccgaac
     4201 ggcgcggcgt tttgtcacgt ctttgcgtag tacttctgca gattctggat cacggtgcgg
     4261 tagttgaccc aatcctgaaa gaacgccgta agcagcaaaa tcacgagcga ttccgctgga
     4321 tccaggttgc ccggaccacc gaagccgaac tgggccgcgg cctg
//
LOCUS       DAOPBQ010000303         4362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16555_length_4362_cov_4.020432, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000303 DAOPBQ010000000
VERSION     DAOPBQ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16555_length_4362_cov_4.020432"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKW60_10020"
     CDS             <1..622
                     /locus_tag="PKW60_10020"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPJ99810.1"
                     /translation="TVIAIDAGHGGADFGCINPAILEKDFTLAVARHTARLLENSFGG
                     RVILTRTQDANPSLEVRARLAKAQGAGLLISIHAGASISPSAHGFELFVPPATHLDAG
                     AGAPPGPRASGARRAAAQSTEIAHRLADELARSTGAVNRGVRAAPCRLFEAVDFPCVV
                     VEVGSLTNYAEASKLAEDEYQMKIAQGLADGIRACAAGAVRNGEAP"
     gene            619..1290
                     /locus_tag="PKW60_10025"
     CDS             619..1290
                     /locus_tag="PKW60_10025"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPJ99811.1"
                     /translation="MKADERQEFVKKALLGLWAMFTLILVVALGLVVVTMVQQGRSPF
                     PAPAVTRAPGAQAPADPQALSQELRETRLYFASEDGRLLEPESRRLTLGDDTIANCHA
                     ALDALIAGPDGVLTPVVPPATRVRGIFFMGNGEMIVDLSMETVAGLRKMGSVSTEMLF
                     LQGIVHTLTAPELAGTDDFTVTKVRFLIEGAPADETFKDAHCTWAYALPRDSQWLGGS
                     SPAGL"
     gene            1292..2095
                     /gene="murI"
                     /locus_tag="PKW60_10030"
     CDS             1292..2095
                     /gene="murI"
                     /locus_tag="PKW60_10030"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPJ99812.1"
                     /translation="MPESSAAIGIFDSGVGGLTVFARIAEDLPNESIIYLGDTARVPY
                     GTKSGETILRYAAGCADVLVERGIKMLVVACNTASAHALDSLRDELAIPVLGVIIPGA
                     RSAVDLSRSGRIGIIGTASTIASKAYEEAILGMAPEASVFSKSCPLFVPLAEEGWLHG
                     AVPRQIAAAYLEELLPHHIDTLVLGCTHYPLLREVIQEAIGPDVTLVDSAQATSRAVA
                     ETLTGLGMKRDAASPPSHTFLVTDAPGAFARVGERFLGRATSPVEWVDV"
     gene            2080..3723
                     /locus_tag="PKW60_10035"
     CDS             2080..3723
                     /locus_tag="PKW60_10035"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPJ99813.1"
                     /translation="MGGRLMSEPVQTPDPGPPMDSAVVRTSLRGLIISIAGLMAVLLL
                     ILVNAVLIRRHIASLPLNLVPATNAMAEHIEDLCRRSHLPPECLKHYEPIARHDDGAE
                     WNEHAFEAGVPATIAAENFIAALTCGMALQQVSMERTDAPQGRWTELRFSMLNRPFAV
                     VRVMGGAERYEYTAACDKLAQTFLAALAHIPDFTDVQETAQHDRQEGPVRWRTYTFRA
                     LARQRIEAGAVRELLATTLPELQGEVPVAVAVPGQDAIEVVWKGLPIVRLDVECGEHL
                     LGETEAGQQTDSPEPQPAETPPSASLSRLAPGAATKTTEKRPAPPKPPVLSGTTPRAA
                     IIVDDGGYGTLASDTILAMDTRLTLAILPDTPFARETAETARSRGFEVLVHLPLESEG
                     AGAAFPEQLTTSMNEDQMLERLDAALARVPGSAGVNNHTGSKFTADASAMQRLLSGIK
                     RRDLFFVDSRTTPDTVAESCARELGVPTASRSVFLDNEPEPEAIRAQAAALVEMAESG
                     VPAIGICHFRASTARLLPEIVRLLEARGVSLVHVSELVQ"
     gene            3720..>4362
                     /gene="tsaD"
                     /locus_tag="PKW60_10040"
     CDS             3720..>4362
                     /gene="tsaD"
                     /locus_tag="PKW60_10040"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPJ99814.1"
                     /translation="MTRVLGIETSCDETGVAIVADGARILANEVASQIDVHAEFGGVV
                     PEIASRQHILCISQLVRKALDDAGCGRDEIAAIAATNGPGLMGSLLVGVCFGKALAYA
                     WRKPFVPVHHIEGHIFSAFLSGDPPAFPFLTLVVSGGHTCLIWCERPHVYRLLGTTRD
                     DAVGECFDKVARLLELPYPGGPSIQRAAESGNPNACDFPRAMSQADTLDFSFSG"
BASE COUNT          749 a         1464 c         1352 g          797 t
ORIGIN      
        1 cacagtaatc gccattgacg cggggcatgg cggcgctgac tttggctgca tcaaccccgc
       61 gattctcgag aaggatttca cgctggcggt tgcacggcac actgcccgtt tgctggaaaa
      121 ttctttcggc gggcgcgtga tccttacccg aacacaggat gccaatcctt ccctggaggt
      181 tcgggcccgc ctggcgaagg cacagggcgc cggcctgctg atcagcattc atgccggggc
      241 aagcatctcg ccttctgccc acgggtttga actctttgtg ccgccggcaa cccatctgga
      301 tgcgggtgcc ggcgcccctc ccggccccag agcctccgga gcccgccgcg ccgccgcaca
      361 gagcacagag atcgcacaca ggctcgcaga cgagcttgcc cgctcgacag gcgcggtcaa
      421 tcgcggcgtg cgcgccgccc cctgccggct cttcgaggca gtcgattttc cctgcgtcgt
      481 cgtcgaggtg gggtcgctta cgaattacgc ggaggcatcg aagctcgccg aggacgagta
      541 tcagatgaaa atcgcacagg ggctcgccga cgggattcgc gcctgcgccg ccggcgccgt
      601 ccggaacgga gaggcgccat gaaagccgat gaacgccagg aatttgtaaa gaaggccctg
      661 ctcggattgt gggccatgtt cacgctgatc ctcgtggtgg ccctcggtct ggtggttgtc
      721 actatggtgc agcagggccg gagtcccttt cccgcgccgg cggtcacacg cgcgccgggc
      781 gctcaggcgc ccgcggatcc gcaggcgctg tctcaggaat tgcgggagac ccgcctctat
      841 ttcgcatcgg aggatggccg gctgctcgag ccggagagcc ggcggcttac cctgggcgat
      901 gatacgatcg cgaattgcca cgcagcgctt gacgcgctta tcgcgggtcc ggatggtgta
      961 ttgaccccgg ttgtcccccc tgcgacccgc gttcgcggga tcttcttcat gggcaacggc
     1021 gaaatgatcg tcgacctctc aatggaaacg gtcgcgggcc tgcgcaaaat ggggagcgtc
     1081 tccactgaaa tgttgttcct tcagggcatc gtccatacgc tcacggcccc ggaattggca
     1141 ggtacggacg acttcaccgt cacgaaagtc cggttcttga ttgaaggggc tcccgcggac
     1201 gaaacattca aggacgcgca ctgcacctgg gcctacgcgc tgccccggga ttcccagtgg
     1261 ctcggcggca gcagcccggc aggcctctga gatgccagaa tcaagcgcgg ccatcggaat
     1321 cttcgattcg ggcgtcggcg gcctgacggt ctttgcacgc atcgcggaag acctcccgaa
     1381 tgaatccatc atttacctgg gcgatacggc ccgggtgccc tacggcacga aatccggcga
     1441 aaccatcctg cgctatgcgg cagggtgcgc ggatgtgctc gtggaacgcg gtatcaagat
     1501 gctcgtcgtt gcgtgcaaca cagcgtccgc gcacgcactc gactcgctgc gtgacgaact
     1561 cgccattccc gtgctcggtg tcattatccc cggcgcgcgg tccgcagtag acctgtcgcg
     1621 gagcggtcgg atcggcatca tcggcacggc cagcaccatc gccagcaagg cgtacgagga
     1681 ggcgattctg ggcatggcgc ccgaggccag cgtcttctcg aaatcctgcc cgctgttcgt
     1741 gccgcttgcg gaagaaggct ggctgcacgg cgccgtcccg cgtcagatag ccgccgccta
     1801 cctcgaagaa ttgctgccgc accacatcga cacgctcgtg ctcgggtgca cacactatcc
     1861 gctgcttcgg gaagtcattc aggaggccat cggcccggat gtgaccctgg ttgacagcgc
     1921 ccaggccacc tcgcgcgcag tggccgaaac gttgaccggg ctgggcatga aacgcgacgc
     1981 ggcgtcccca cccagccata catttctcgt gaccgatgcg cccggcgcct ttgcgcgggt
     2041 cggagagcgc tttctcggcc gcgccaccag tcccgtcgaa tgggtggacg tctaatgtcc
     2101 gagcccgtgc aaaccccgga ccccggcccg cccatggact cagccgtcgt gcgcacctct
     2161 ttacgcgggt tgatcatctc gatcgccggc ctgatggccg tgctcctgct gatcctggtc
     2221 aacgccgtgc tgatacgccg ccacattgcc tcgctcccgc tgaatctggt gcccgccacg
     2281 aatgccatgg ccgagcacat tgaagacctg tgcaggcgaa gccacttgcc tccggaatgc
     2341 ctcaaacact atgaacccat tgcgcgtcac gatgatgggg cggaatggaa tgaacacgcc
     2401 ttcgaggctg gcgttcccgc caccatcgcg gcagagaact tcatcgcggc gttgacctgc
     2461 ggcatggcgc tccaacaggt ctcgatggag cgcacggacg cgcctcaggg ccgctggacc
     2521 gaactgcgtt tttccatgct taaccggccg tttgcggtgg tgcgggtcat gggcggcgcc
     2581 gagcgctatg aatacacagc cgcctgcgac aaactcgcgc agaccttcct cgcggcgctg
     2641 gcgcacattc ccgatttcac ggacgtgcag gagaccgcac agcacgatcg gcaggaaggt
     2701 cccgtacgct ggcgcacgta tacattccgg gcgctcgccc ggcagagaat cgaggccggc
     2761 gccgtgcgcg aattgctcgc caccaccctt ccggaactcc agggagaagt tccggtggcc
     2821 gtcgccgttc ccgggcagga tgccattgaa gtcgtgtgga aaggccttcc cattgtccgg
     2881 ctggatgtcg agtgcggcga gcacctgttg ggtgagacgg aggccgggca gcaaacggat
     2941 tcccccgagc ctcaacctgc ggagacgcca ccctccgcca gtctgtcccg gcttgccccg
     3001 ggagccgcca cgaagactac cgaaaagcgg ccggcgcccc caaaaccgcc agtcctttcc
     3061 ggcacaacgc cccgcgccgc cattatcgta gacgatggcg gttatggcac gctcgcatcc
     3121 gacaccattc tggccatgga cacccgtctg acactcgcca tccttccgga cacgccgttc
     3181 gcgcgcgaga ctgccgagac cgcgcgctcc cggggattcg aggtgctggt ccatctgccg
     3241 ctcgaatccg agggcgccgg ggctgcattt cccgagcagc tcaccacgtc catgaacgaa
     3301 gaccaaatgc tggagcgcct ggatgcggct ctcgcccgcg taccgggctc cgctggcgtg
     3361 aataatcaca cgggctcaaa attcacggcg gatgcctctg cgatgcagcg cctcctcagc
     3421 ggcatcaagc gccgcgacct cttttttgtc gacagccgga ccactccgga caccgtcgcg
     3481 gaaagctgcg cccgtgaact cggcgtaccc acggccagcc gctcggtatt tctcgataac
     3541 gaacctgaac ctgaggccat ccgtgcacag gcggccgcgc tggtggaaat ggcggaatcc
     3601 ggcgttcccg cgattggcat ctgccatttc cgggcttcca ccgccagact gcttcctgaa
     3661 atcgtgcgcc ttttggaagc ccgcggcgtg tcgctcgtgc atgtgtcgga gctggtgcaa
     3721 tgacacgcgt gctcggaatt gagacctcct gcgatgaaac cggcgtcgct atcgtggccg
     3781 acggcgcccg aatcctggcc aatgaagtcg cctcgcaaat cgacgtgcac gcagaattcg
     3841 gcggcgtggt gccggaaatt gcttcgcgcc agcacattct gtgcatctcc caactggtgc
     3901 ggaaagctct ggatgatgcg ggctgcggcc gggacgaaat tgcggccatt gcggcaacca
     3961 acggcccggg cctcatgggt tcactgctgg ttggcgtctg ttttggcaag gcgctggcct
     4021 acgcctggcg caagccgttc gtgccggtcc accatatcga gggccacatt ttctcggctt
     4081 ttctcagcgg cgatccgcct gcgtttccct ttttgaccct ggtcgtcagc ggcggccaca
     4141 cctgcctgat ctggtgcgag cgcccgcatg tgtaccggct tctcggaact acgcgagacg
     4201 atgccgtggg cgaatgtttt gacaaggtgg cccgtttgct cgagctgccc tatcccggcg
     4261 gaccctcgat tcaacgagcg gcggaatccg gcaacccgaa cgcctgtgat tttccccgcg
     4321 ccatgagcca ggccgacact ctcgatttca gcttcagcgg cc
//
LOCUS       DAOPBQ010000304         4360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16564_length_4360_cov_4.470616, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000304 DAOPBQ010000000
VERSION     DAOPBQ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16564_length_4360_cov_4.470616"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /locus_tag="PKW60_10045"
     CDS             complement(<1..1627)
                     /locus_tag="PKW60_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HPJ99815.1"
                     /translation="MSRRNKKQEQTLTMQAQPTRHDLSQPASSDPNGIKPRPARLVCT
                     ERQWAAFRNHLIQSDGLEHAAFALLGRSDTGDAIKYYMHRLLLVPDDRCVQQHAAFVE
                     PEIDFILECFQKYIQSGAAAILHAHSHPFTPRATFSGTDDYYLPGEIDSLASVVAMMA
                     SDVPRRFARIVLGQSEDGFTAEVYSEDHIIRERIVAVDVIGKSGIRTIVCQGRHPAPG
                     LADATDALTEADRQRLDRNLNWLGEVGQKKIAETHIALCGLGGLGAEVMKNCRGLGIK
                     RYTLIDMDRIEITNMNRLPYPATSVGQYKVDEARKFLLEVIPDAEVIALRCPVEDPAA
                     QEALLKADLIVNSLDDDGARFSTQILAARHLIPLLDLGSGIALKAGHREVTAMGGQAI
                     FYAPGEACLVCQGLDPARIVSREFRKFYRQIGYVQGTDETPTSVVTINSVMGGVAGDI
                     IMKYLTGFSSIPSWVACDLLQHETQTLSFVRRDDCPICGEHGIEGLGTDMTKPIESPG
                     QPSRPFSMPFVEESSQDPVQTPETSVTPEIPPAA"
     gene            complement(2230..2901)
                     /locus_tag="PKW60_10050"
     CDS             complement(2230..2901)
                     /locus_tag="PKW60_10050"
                     /inference="COORDINATES: protein motif:HMM:NF025817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HPJ99816.1"
                     /translation="MQNNAKNNQTASRNIHFRGQNFAVPVNSQGIVTGEDIKDHLGLS
                     DDTILVRDTGSGFQSVQPSDEVADGARCAAVGRFATAGRDIRRLNAELQLLMTMYGND
                     RVSWPRTQNWVMIRDFPLPAGWNFKTSDIVVILPDNYGFGEPLKHCFVDPALRFLHNG
                     NWKKIDHYFDSERGYTPHPEFNAKGWRYLCLHMKTWTPDSWVQTYLDAITTFLSDPFA
                     GWPTS"
     gene            complement(3051..3557)
                     /locus_tag="PKW60_10055"
     CDS             complement(3051..3557)
                     /locus_tag="PKW60_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99817.1"
                     /translation="MHKHDAENETTHRQETAVTEMTQDSARDWSVRYAPVPETTNPED
                     AGAQTERVAAESADAASIARRVKLPHGFHLTAHAWVRMTARAIPTVAVEMALAYGRKV
                     RVHGARYYVLGRREIDRARRSGLDFRQYEGIQVVASAEQPIIYTVYRNRDFKHTLRAA
                     RRPWRTHH"
     gene            complement(3631..4023)
                     /locus_tag="PKW60_10060"
     CDS             complement(3631..4023)
                     /locus_tag="PKW60_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99818.1"
                     /translation="MNRGLTGVFHTSSDMLCKIAADLRKRYGDADAEVFLFRRANTAA
                     QAHKGKLSEILRPFLQFCQEFPYSQVDYQPLHPYGLVCRVLCNCHMRDTPCRIWCHTA
                     EERPNAMVLLTRVNGEDIALGGRGTPPQ"
     gene            complement(4137..>4360)
                     /locus_tag="PKW60_10065"
     CDS             complement(4137..>4360)
                     /locus_tag="PKW60_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99819.1"
                     /translation="NGATIYAPVRLANGLTGLSKGTATASLFLGPKYLGLKSPERVRG
                     ILCCEQHLKGFADTVLLKLAPARPESVVN"
BASE COUNT          826 a         1268 c         1292 g          974 t
ORIGIN      
        1 cggctgccgg ggggatctcg ggcgtcacag aggtctcagg tgtctgcaca gggtcttgcg
       61 acgattcctc gacgaaaggc atcgaaaatg ggcgcgaggg ctggcccggg gattcgatgg
      121 gcttcgtcat gtccgtgccc agaccttcga tcccgtgctc cccgcaaatg ggacagtcat
      181 cgcggcggac aaaggacagg gtttgtgtct catgctggag aagatcgcag gccacccagg
      241 aaggaatgct gctgaatcca gtcagatact tcatgataat atcaccggcg acaccaccca
      301 tgacggaatt gatggtaacc acagacgtgg gcgtttcgtc cgtaccctgt acgtagccta
      361 tctgccggta gaattttcgg aactctctgg agactattct tgcggggtca aggccctggc
      421 aaacgagaca cgcctcccct ggggcgtaga agatcgcctg cccgcccatg gcggtcactt
      481 cacggtggcc cgctttgagc gcgatgcccg accccaggtc aagcagcggg atgaggtggc
      541 gtgcggcaag tatttgagtg ctgaagcgcg ctccgtcgtc atccagacta ttgacgatga
      601 gatcggcttt gaggagcgcc tcctgcgcgg cggggtcctc caccggacac cgaagtgcga
      661 tcacttcagc gtcggggatg acttcgagca ggaacttgcg cgcctcgtcc accttatatt
      721 gcccgacact cgttgccgga tacggcagac ggttcatgtt cgtaatttct atgcgatcca
      781 tgtcgatcag cgtgtatcgc ttgatgccca agccacggca gtttttcatc acttccgcgc
      841 cgagcccgcc caacccgcac aatgcaatat gggtctccgc aatcttcttc tgtccaactt
      901 cgcccagcca gttgaggttg cgatccaacc gctgccggtc cgcttccgtc aacgcatcgg
      961 tcgcgtcggc tagcccaggc gcagggtgtc ttccctggca tacgatggtt cggatcccgc
     1021 tcttcccaat cacgtccacg gcaacgatgc gctcacgtat gatatggtct tcagaataaa
     1081 cctcggccgt gaaaccgtcc tcgctctgcc cgagcactat tctggcgaaa cggcgaggta
     1141 cgtctgacgc catcatggcc acaacgctcg caaggctgtc tatttcgccg ggaagatagt
     1201 aatcgtccgt cccgctgaag gtcgcgcgtg gtgtaaaggg gtggctgtgc gcgtgcagga
     1261 tcgcagctgc gccgctctgg atgtatttct ggaaacactc caggatgaaa tcaatttcgg
     1321 gctcgacgaa ggcagcgtgc tgctggacgc accggtcgtc gggcacaagc aagagacggt
     1381 gcatgtaata cttgatcgcg tcgccggtgt cgcttcgtcc gagcagcgca aatgcggcat
     1441 gttcaaggcc gtccgattgg atgaggtggt tgcgaaaggc cgcccactgc cgttcggtgc
     1501 acaccagcct cgcgggtctg ggtttaatcc cgttcgggtc agagctggcg ggctgcgaaa
     1561 gatcgtgcct tgtcggttgc gcctgcatag tgagtgtctg ctcctgcttc ttattgcgtc
     1621 tgctcaacgt acgcattccg agccggaacc gtacgatatg ggccaggcgg cgaaggagtc
     1681 gccgagaagc gcggtgagcg cgtccaggct gtgctgcttc ctccagtcct gctttcatcc
     1741 cttcagatgc aggctctggt atcgccgcct cggcttgttc aagtcccggg cgctgctgca
     1801 gtttcttgtt cagatggccg gtgttttggc cggctgtgat gaaactgctc acggtcccaa
     1861 accaggtatc cgaagctgga tctccttggt ttggcaccat caccccgccc atttctggat
     1921 ctgtgatagc cccgccgaag actgggagat ccacccgcac gacaaacttc tggcctgcga
     1981 agcaaatggt tctgaattcg gtcggattgt cttgcgcatt gttctgcatg gctgtgttct
     2041 ccctggggat ttgaagatcc aagacccttt aaagcaatag gcaagtgaca aagaggagcg
     2101 actggaacgt cgagcgctcc tgtttccatg ctcaagcggc aggccacatc ttgtaagctg
     2161 ctgcccagcg aggacgtcgc ttattttttt catggcgcct gatcagcgcg cagattgcgc
     2221 tacggggccc tacgaggtgg gccagccggc gaaagggtcg ctgagaaacg tggtgatcgc
     2281 gtccagataa gtctgcaccc acgaatccgg agtccaggtc ttcatatgca ggcacaggta
     2341 gcgccatccc ttcgcattga attccgggtg aggggtatac cctctttcgc tgtcgaaata
     2401 gtggtcgatc ttcttccagt tcccgttgtg gagaaatcgc agcgccgggt cgacaaagca
     2461 gtgtttcaat ggctcaccga agccgtaatt gtctggcagg atcaccacga tgtcggaggt
     2521 cttaaagttc caccccgctg gcaggggaaa gtctcttatc atcacccagt tctgagtgcg
     2581 gggccacgac acgcggtcat tcccatacat cgtcatgagc aactgcaatt ccgcgttcag
     2641 gcggcggata tcccggccgg cagtggcaaa gcgtcccacg gcagcgcatc gcgcaccatc
     2701 ggctacctcg tcggacggct gcaccgactg gaagccgctt ccggtgtccc gcacgagtat
     2761 ggtgtcgtct gaaagtccaa ggtgatcctt gatgtcctcg cccgtgacaa tgccctggga
     2821 attcaccggc acggcaaagt tctggcctct gaagtgaatg tttctggaag cagtctgatt
     2881 gtttttcgcg ttgttctgca tggctgtgtt ctccttgcgg tttggaggtt ccaaagtcct
     2941 tcaaaacaat aagcaagtga caaagaaggg cgcctggaac gtcgggcgcc tctcggtttc
     3001 catgctcagg cggcgggcca ggcgcacact cggcagcgcc cgaacgtacg tcagtggtgc
     3061 gtccgccagg ggcggcgggc cgcccggagg gtgtgtttga aatcgcggtt gcgatatacg
     3121 gtgtagatta tcggctgttc ggcggaggcg accacctgga tgccttcata ctggcgaaaa
     3181 tccaggccgc ttcgtctcgc ccggtctatc tcgcgcctcc caagcacgta ataccgggct
     3241 ccgtgtacgc gcaccttccg cccgtaagcc aaagccatct ccaccgccac ggttggaatg
     3301 gctctcgccg tcatgcgcac ccaggcatgg gcagttaaat gaaatccatg cggcaatttc
     3361 actctgcgcg caatggaggc cgcgtcggcg ctttcggccg ccacgcgctc cgtctgcgca
     3421 ccggcatctt cgggattcgt tgtttccggt acgggggcat agcgtacgct ccagtcccgc
     3481 gcggagtcct gcgtcatttc cgtcactgcc gtttcctgtc tgtgagtcgt ctcgttttcc
     3541 gcatcgtgtt tgtgcatggc tctgcgctcc tttgggtttg ctgggttgac gcctttcaca
     3601 gggataaaaa actgacaaat taccaagcgc tcattgaggg ggtgttcccc ggccgccaag
     3661 ggcgatgtcc tcgccgttta cacgggtgag gagcaccatg gcgttcgggc gctcctctgc
     3721 ggtgtggcac cagatgcggc aaggggtatc ccgcatatga caattgcaca agacccggca
     3781 gaccagcccg taggggtgca ggggctggta gtccacctgg gaatagggga attcctggca
     3841 gaactggagg aacggtcgca ggatctccga cagttttccc ttatgcgctt gtgccgctgt
     3901 gtttgcccgg cggaagagaa agacctcggc gtcggcatca ccgtagcgtt ttcgtagatc
     3961 ggcggctatt ttgcacagca tgtcacttga tgtgtggaag actcccgtaa gccccctgtt
     4021 catcagcgcc tcctttgcgt ggttctgagc tcggcccggg cccgcgggtg ggctcatacc
     4081 caaaagcaac cgacaatcaa acgaattctt acaggggcgt aaagtacttc taataatcag
     4141 ttgacgacgc tttctgggcg ggcgggcgcg agctttaaca ggaccgtatc tgcgaaaccc
     4201 ttcaggtgtt gttcacagca gagaatcccg cgaacccgct ccggagactt gagtcccaga
     4261 tattttgggc caaggaatag cgaagcggtc gcagtgcctt tcgacaggcc tgtcaagcca
     4321 tttgctaatc ttaccggggc gtatatcgtc gcgccatttg
//
LOCUS       DAOPBQ010000305         4359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16568_length_4359_cov_4.772537, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000305 DAOPBQ010000000
VERSION     DAOPBQ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16568_length_4359_cov_4.772537"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PKW60_10070"
     CDS             <1..613
                     /locus_tag="PKW60_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99820.1"
                     /translation="PIVAVDCAEGWMGSNAIDNSYHYMGYAFDNIGMDNPATQVPSST
                     LNPLILAFGGTPPATVGPVPVQAVAWIYAVATELQSFSALNSWGTLNDYSTVGLLGGA
                     VSFPGAGNSKGNTLYKLREGIERFMITDINNPSGSAMAQSELAVMWDTVSTEVKEYNH
                     IPGGSNVLFMDGHVEFQRYSQNGAGPVNGGFALVVGVLNTADL"
     gene            1006..1968
                     /locus_tag="PKW60_10075"
                     /pseudo
     CDS             1006..1968
                     /locus_tag="PKW60_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200926.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
     gene            2266..3045
                     /gene="kdsB"
                     /locus_tag="PKW60_10080"
     CDS             2266..3045
                     /gene="kdsB"
                     /locus_tag="PKW60_10080"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPJ99821.1"
                     /translation="MSESTAPVVVGVVPARYASTRFEGKVIAPLSGKPVVMHVYERVM
                     QSQRISSVVIATEDERVRQALQPYSANVVLTRADHPSGTDRVAEVVAALDADIVVNVQ
                     GDEPLIDPRLIDAAIQPLLDDPAVAMATARRRIQDQDFLEDPNVVKVVCDKRGRALYF
                     SRWPVPYVRGQSPDVSLGCYWQHIGLYVYRKDFLLAYAKMAPTPLEQAEKLEQLRALE
                     NGHTIAVVETEYASVGVDTPEDLQRVQQLLQQEQGENRAHA"
     gene            3038..>4359
                     /locus_tag="PKW60_10085"
     CDS             3038..>4359
                     /locus_tag="PKW60_10085"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPJ99822.1"
                     /translation="MPSYVFITGGVVSSVGKGIAAASLGLLLEARGLKVAMMKMDPYI
                     NVDPGTMNPHEHGEVFVTDDGAETDLDLGHYQRFTRAKLSRKSNVTTGSVYNAVIQKE
                     RRGEYLGKTVQVIPHITDEIKSRIRMLTAEPEDEADVAIVEIGGTVGDIESLPFLEAI
                     RQFNLEVKPTPCCFVHVTLLPYIDAAGELKTKPTQHSVRALRDIGIQPDLICCRTGHR
                     TLGPDQRRKIAMFCNVTENAIIGAPDISPVYAIPLNLHEQGLDQTVLDVLQLDVPPAD
                     LDQWRGLVEHMTCPNEEIHVAAVGKHVAHDDAYKSLREALCHAGTANGCKVRLEWIDS
                     EQFENGISPEDVLKDFDGILIGSSIGKRGVEGKIRAVQYLRESKKPFFGIGLGMQIAC
                     IEIARNLLGLAGANSTEFEPETPHPVISLLTEQKGVKDMGGTMRLGAYR"
BASE COUNT          931 a         1231 c         1322 g          875 t
ORIGIN      
        1 gccgattgtc gcggtggatt gcgccgaggg ctggatgggt tcgaacgcga tcgacaatag
       61 ctaccactac atgggctacg cctttgacaa catcggcatg gacaaccccg cgacccaggt
      121 tccctcatcg accttgaacc cgctcattct ggcctttggc ggcacgccgc cggcaacggt
      181 cgggccggtg cctgttcagg cggtcgcgtg gatctatgcc gtcgccactg agctccagag
      241 cttttccgct ctgaattcgt ggggaacgtt gaacgattac agcaccgtcg gcttgctggg
      301 cggcgcggtg tcgtttcccg gcgcaggcaa tagcaagggc aataccctct acaagctgcg
      361 cgaaggcatc gaacggttca tgatcaccga catcaacaat cccagcgggt cggcgatggc
      421 ccagagtgag ctcgcggtca tgtgggatac cgtatcgacc gaagtcaagg aatacaacca
      481 catccccggc ggttcgaatg tgctgttcat ggacgggcat gtggaatttc aacgctactc
      541 gcagaacggc gcaggtccgg taaacggcgg atttgcgctg gtagtcggcg tgctgaacac
      601 ggctgacctg taatctgtac tatcaacggt ttatagcgtg gcaatgaccg attgcgcgcg
      661 gtccgtggcc cccgggctgc ggaccgcgct cgttttccct ccttttcggg cgaggccggt
      721 gcgctcgtaa gccttgtttc ggagatgttt cgctccacgc attctgcctg ggctcgaagt
      781 gcctatgtcc aggtgcggct ccaatggcac ctgcctccca gtcccaaaac gccataagat
      841 cttggcggat aatatcttta tgcgcactgt agaaatagca cttgcagcaa tgccccacat
      901 ggagtagtat tattatcagc agaggcgctg ggaaagtaat cgatttcctg cgccgtgatt
      961 tggattggga taatcacaaa cagacaggga gactacgtgc aatgaaaaag aggattcacg
     1021 ctgattgaat tgctggtcgt cattgccatc atcggcatcc ttgcagccat ccttcttccc
     1081 gcactggccc gtgcacggga agccgctcgc agagcaagct gcgccaataa cctgaaacag
     1141 ctcggactcg ttttcaagat gtatgcgaac gaaagcaaag gggagaagta tccgacggta
     1201 gcgcagttcc ggaatacatt gcgggatgag ggcgttccgc gggttctgtg ccaggctggc
     1261 gctcaggcgg gccagcctta cacgctgtgg gcggaagata acggtcgccc ggcctcgtac
     1321 tacttcgcct acattccgca gatttacccg gaatacctga ccgatgccaa cgttctcatt
     1381 tgcccgtcag aagcggaccg tccgattata tttgatccgg ttacgggcga gagcatcctg
     1441 catctggcct gtgataactt cggcatgggg caggcggccg ccgacgaatc ttactattac
     1501 accgggtttt gcctggatcg tgcagactct aaagacttgg acggtggcct gatgggcggc
     1561 acgccggggc tgtatatttc cttgcaggtt ggcggtctct taaccctgct cggcaatctc
     1621 cagactttcg cgggtgccga caacgatgtg aatatggcat ccatcacttt ccccggcctc
     1681 ggcactctgg ctggctatgg aaacgcgggc ggggacacgg tgtaccgtct tcgcgaaggc
     1741 attgagcgct tcctggtgac ggacatcaat aacccggccg ggaccgccat ggctcagagt
     1801 gaactgccca ttgcggcgga ccttgtttcg acgaatgtgg aacagttcaa ccacgtgccc
     1861 ggcggctgca acgtgctgtt catggacggc cacgttgaat ttgaacgtta tccgggcgat
     1921 cacattgtga gtgaggccat ggcagccacc atcggcatcg ccggctaata ctttcgaatc
     1981 gaacccgatt atcccaatcc ctgctgtccc ccggaactcc cgttgagagt ttcgggggac
     2041 gttttacagg cagcctcaga ttttctccct tccgaaggag ttacccgcgg acatagcctg
     2101 cgccgcggcc cgcctcgaag aggctccgaa ttgaaaaagc cgagcaggta tggtagattg
     2161 acgtcccgat ggggagcggg acgccttcct tttatcctaa cccatttaca ctcaaaccgt
     2221 tagaactgcg catgcaatac aggcccacgc gcgaggagta aggtcatgtc tgaatcaact
     2281 gcgcccgtgg tagtgggggt agtccccgcc cggtacgcct caacccgctt cgaaggcaaa
     2341 gtcattgcgc cactgtcagg aaagccggtc gttatgcatg tctatgagcg ggtgatgcag
     2401 tcgcaacgca tctcgagcgt agtgatcgcg accgaggacg agcgcgtccg gcaggctctg
     2461 cagccgtaca gtgcgaatgt tgtgttgacc cgggccgacc atcccagcgg gacggaccgc
     2521 gtagccgaag tggtggcagc gctggatgcg gacattgtcg taaacgtcca gggtgatgaa
     2581 cccctgattg acccccggct gatagacgct gccatccagc cgctcctgga tgaccctgcg
     2641 gtcgccatgg caaccgcacg ccgccgcatt caagaccagg acttcctgga ggacccgaat
     2701 gtggtgaagg tggtgtgtga caagcggggg cgggcacttt atttttcacg gtggccggtc
     2761 ccgtacgtgc gcggccaatc gcctgacgta tccctggggt gttactggca gcatattggt
     2821 ctgtatgtgt accggaaaga ttttctactc gcctatgcga aaatggcgcc gactcccctg
     2881 gaacaggcgg aaaaactgga gcagttgcgc gcgctggaaa acgggcacac tatcgcggtg
     2941 gtggagacgg aatatgcaag cgtgggcgta gacacacccg aagacctgca gcgggtgcaa
     3001 cagttgctgc aacaggaaca gggagaaaac cgcgctcatg cctagctacg tattcatcac
     3061 gggcggagtg gtctcgtcgg tggggaaagg catcgcggcg gccagcctgg ggcttctgct
     3121 cgaagcccgc ggactcaaag tggccatgat gaagatggat ccgtatatca acgtggatcc
     3181 gggcacgatg aacccgcacg aacacggcga ggtgttcgtc acggatgatg gagcggagac
     3241 tgacctcgac ctgggacatt accagcggtt tacccgcgcg aagttgtcgc gtaaaagcaa
     3301 cgttacaaca ggcagcgtgt acaacgcggt cattcagaaa gagcgccggg gcgaatacct
     3361 ggggaagacg gtacaggtaa tcccgcacat cacggatgag atcaaatccc gaatccggat
     3421 gctgacggca gagccggaag acgaagctga tgtggccatc gtcgagatcg gcggaacggt
     3481 cggcgacatc gagagcctgc cgttcctgga ggcgatccgg cagtttaacc tggaggtcaa
     3541 gccgacccct tgctgttttg tgcacgttac tttgcttccg tatatcgacg cggcgggcga
     3601 gctgaagacc aaaccaaccc agcacagcgt ccgcgcactg cgggacatcg gcatccagcc
     3661 ggacttgatt tgctgccgga ccggccaccg cacgctgggc ccggaccagc gccggaagat
     3721 cgccatgttc tgcaacgtta ccgaaaatgc gattatcgga gcgccggaca tttcgcccgt
     3781 ttacgccatc ccattgaatc ttcatgagca gggactggac cagaccgtgc tggatgtgct
     3841 ccagctcgat gtgccgcccg cagacctcga tcaatggcgc ggcctggtcg agcacatgac
     3901 ctgcccgaac gaagagattc acgtagcggc ggttggcaag cacgtagccc acgacgacgc
     3961 ctacaagagc ctccgcgagg cgctctgcca cgccgggacg gccaacggct gcaaggttcg
     4021 gttggagtgg attgactccg aacagtttga aaacgggatt tcgcctgaag acgtgttaaa
     4081 agacttcgat ggcatcctga tcggatcgag tatcgggaaa cggggtgtag agggcaagat
     4141 tcgtgcggta caatacctgc gcgagagtaa gaagcctttt ttcgggattg gcctgggcat
     4201 gcaaattgcc tgcattgaaa ttgcccggaa tctgctagga ttagcgggag cgaactcgac
     4261 ggagttcgaa ccggaaaccc cgcatccggt catcagtctg ttgaccgagc agaaaggggt
     4321 caaagacatg ggcggcacca tgcgcctggg cgcctaccg
//
LOCUS       DAOPBQ010000306         4358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16575_length_4358_cov_3.660237, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000306 DAOPBQ010000000
VERSION     DAOPBQ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16575_length_4358_cov_3.660237"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1482)
                     /locus_tag="PKW60_10090"
     CDS             complement(130..1482)
                     /locus_tag="PKW60_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99823.1"
                     /translation="MITFYCPKCTKQFSAPDSTVGQYGWCQDCGSIVRVPQSANALNP
                     EAVHVTLDPEILALLGRMVAQIKEYQERARRAEASVRAMRTGRKNLTALEQQLEKAEA
                     LVSTLAAQMSGGKKDHHQIEAELHEELQRERAAHAGARAHVHELKARLAAAAKESEER
                     AEEISRLGARQTHFEERMTELAVRSGKLEEYAHALRRDRDEARHLLESHRIAEPGSET
                     IVVRLQEKEAAIQRLSEELRLALEKKGEAESMVRQRLDDIEHLSAELSGVTQSAEAEI
                     LEIQAELRAEREAHLLAERALVAAQEELRSLRLRLDALYEGGSAPQSPKAGAARVVET
                     SETAGERQASADAREEELHNARERIRALETELERMRKRHHSAPDISDDPTELIEEEPQ
                     PPRAEMSGKDGPFAGPFLAPSSQKSTVQGIALIPEVMDEDFDDQEMLDTLMRFLQSEE
                     "
     gene            1663..2922
                     /locus_tag="PKW60_10095"
     CDS             1663..2922
                     /locus_tag="PKW60_10095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPJ99824.1"
                     /translation="MKRPVAAETLLNIAYLAPELGALTSTFVYREVEALRQRNVSIAL
                     FSTVQPDAARISDEAKPVVAETQYLYNGPQTALIGAVSGYFVRHPLRFARVLALAVRD
                     ALISRTPSAQDRLKMLWHFLLGCRLANLLMSRGCQHVHAHFAHVPAAIAMYGGLLAAV
                     PFSFTAHANDLYERGTALREKVRRAAFTAVISDYNRRFLAEEGCLTQRIHIVHCGLDV
                     TRYLFRPASPANTPPFLFSVGRFVEKKGFHVLVEALARLKDRGKQFHCVIAGDGPLYE
                     ETRDRAQAAGLLDSLELPGAMPQERVKELLQAADAFVLPCVVAKSGDRDGIPVALMEA
                     MALGVPVVSTDVSGIPELIQDGENGLLMSEGDIDALAQALERILDEAPLRESLARRAR
                     TTIEMEFNLETIAGNLERLFRESVYGE"
     gene            2940..3872
                     /locus_tag="PKW60_10100"
     CDS             2940..3872
                     /locus_tag="PKW60_10100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPJ99825.1"
                     /translation="MTAVPDAASSGRAYVVIITARDEARYLQGTIDSVAGQTLLPVEM
                     VIVNDGSRDETGAIADAAAAKYSWVHVVHREDRGERKVGGGVVETFYAGYEALRTQDF
                     VYQCKLDGDLTLPPGYFEGIIRKMEADPALGGASGKVFNPVGGGAVKEERIIDDMVSG
                     AVNFWRRACWDQIDGYVRMVMWDGIAMHRARMFGWRTRSFRDKDLEIIHHRLMGSSHK
                     NILHGRMRWGRGQWFMGCHPLYILASGINRMFERPYMLGGICIVAGYFSSMLQGAPRY
                     EDLEFRRHLHAWQLKRLGLGFLAPHVTPHERSTA"
     gene            3917..>4358
                     /locus_tag="PKW60_10105"
     CDS             3917..>4358
                     /locus_tag="PKW60_10105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPJ99826.1"
                     /translation="MSFVVIGYNEAATLKACLESVKTANLDGFSWELIYVDGGSTDDS
                     MEIARETGVNLLLGGEMRRRAAENRNLGLSAARGRLVQFIDGDMTLSPDWPRTAAQFL
                     SENEAVAAVCGNLEESAPGVFYAALQIDWAQREGEIRHCGGAAMY"
BASE COUNT          790 a         1300 c         1298 g          970 t
ORIGIN      
        1 caccagcatg cgttgaccgc acgagccgca tgtcaagatg attttcttca ttcaggcgtc
       61 ttctgcattc cgcttggttg tttcttcgat agtatatcca aatcgcccca aaaacacacc
      121 tgtaacgccc tattcttccg actgcagaaa gcgcatgagg gtatccagca tctcctggtc
      181 gtcgaaatct tcgtccatga cttcagggat cagcgcaatg ccctgcacag tggatttctg
      241 gcttgaaggc gccaaaaaag gtcccgcgaa tggaccatct tttccggaca tctccgcgcg
      301 gggcggctgc ggttcctctt caatcagttc agtagggtca tcgctgatgt ccggggcgga
      361 atgatgcctc tttcgcatgc gctccagttc ggtttcaagg gctcggatgc gttcccgggc
      421 gttgtgcaat tcttcttccc gcgcatcggc ggatgcctgc ctctcccctg ctgtttcgct
      481 ggtttccacc acccgggcgg cgccggcctt cggactctga ggggctgagc ccccttcgta
      541 aagcgcgtcc aggcggagcc tgagagatcg cagttcttcc tgtgccgcta ccagggcccg
      601 ttcggccagg aggtgcgctt cacgctcggc gcgcagctcc gcctgaatct cgaggatctc
      661 ggcttcggcg ctttgcgtca cccccgacag ttccgcggag aggtgctcaa tgtcgtccag
      721 gcgctgccgg accatggact cggcttcgcc ttttttctcc agtgcaagcc tgagctcttc
      781 cgaaagccgc tggatggcag cctctttctc ctggaggcgc acgacgatcg tttctgaccc
      841 aggctccgcg atccggtgac tttcgaggag gtgccgggcc tcgtcccggt cccggcggag
      901 ggcgtgtgca tattcctcca gcttgccgct gcgcaccgcc agttccgtca tgcgttcctc
      961 gaaatgcgtt tggcgcgctc cgagacgcga gatctcttcc gcgcgctctt cgctctcttt
     1021 cgccgctgcg gccaggcggg ctttaagttc atgtacgtgg gctcttgctc cggcatgcgc
     1081 tgcacgttcg cgttgcagtt cttcgtgcaa ctccgcctcg atctgatgat gatctttctt
     1141 gccacccgac atctgtgcgg caagcgtact gacaagggct tctgcctttt ccaactgctg
     1201 ttccagggcc gtcaggtttt tccgcccggt acgcatcgcg cgcacgctgg cttccgcgcg
     1261 cctcgcccgt tcctggtatt ccttaatctg ggcgaccatt ctgcccagga gagccaggat
     1321 ctccgggtcc agcgtcacat gcaccgcctc cgggttcagg gcattggcgg attgcggcac
     1381 gcggacaatg cttccacagt cctggcacca cccgtactgc ccgactgttg agtcaggtgc
     1441 actgaattgc ttggtgcatt tcgggcagta gaaagttatc attccggatc gtcctccgta
     1501 tctgggctcc ggacacagcg aaagcccctg ccggcttgct tccagtatac cagagcggaa
     1561 cctccgcccg ggcaggaaaa catgcggaat attgaagcgc aatcatcctg caggtagact
     1621 gtgggttcgt ttacagtaaa gaatcacagg aatggcaatc gcatgaagcg gcccgtggct
     1681 gcggagacac tgttgaatat cgcctacctg gcgcccgagc tcggagcgct cacctccacc
     1741 tttgtctatc gggaagtgga agccctccgg caacggaacg tttctattgc gcttttttca
     1801 acggttcagc ccgatgcggc gcggatctcg gatgaagcca aacccgtggt ggcggagaca
     1861 cagtacctct acaacgggcc ccaaacggcc ctcatcggcg ctgtttccgg ctattttgtg
     1921 cgccatcctc tccgtttcgc ccgcgtgctg gccctggccg tgcgggatgc cctgatttcg
     1981 cgcacgccgt cggcacagga tcgattgaaa atgctgtggc atttcctttt ggggtgccgc
     2041 ctggcgaatc tgctcatgtc gcgtggctgc cagcacgtcc atgcccactt tgcgcatgtg
     2101 ccggccgcca tcgccatgta tgggggactt ctggccgctg tacccttcag ttttactgcc
     2161 cacgccaacg acctgtatga gcgcggcacc gcgttaagag aaaaagtgcg gcgcgccgct
     2221 tttactgcgg ttatttcaga ctacaaccgg cgttttctgg cggaagaagg ctgcctcacg
     2281 caacgtattc atatagtgca ttgcggcctg gatgtgactc gctacctgtt ccgcccggct
     2341 tcgccggcta acaccccccc ctttctcttt tccgtgggcc gtttcgtgga aaagaagggg
     2401 tttcatgtgt tggtggaggc attggcccgc ctcaaagacc gcggcaagca atttcactgt
     2461 gtgattgcgg gggatggccc gctatacgaa gagactcgcg accgcgcaca ggcggcaggc
     2521 ttgctggaca gcctggaact gcccggagca atgccccagg aacgcgtgaa agagcttttg
     2581 caggcagcgg acgccttcgt gcttccctgc gttgtggcga agagcgggga tcgcgacggc
     2641 attcccgtag cgctcatgga ggccatggcg cttggcgtcc ctgttgtctc tacagacgtg
     2701 tcgggcatcc ccgagctgat tcaggacggc gaaaacgggc tgctgatgtc ggaaggagat
     2761 atcgacgcac tggcgcaggc gctcgaacgc atcctcgatg aggcgccgtt gcgggagtcg
     2821 cttgcgcggc gcgcccggac aaccattgaa atggaattca atcttgaaac cattgccggg
     2881 aatctggaac gcttgttccg ggaatccgtc tacggagaat aaaataccgg aggtcaggaa
     2941 tgaccgcagt accagacgca gcatcatccg ggcgggcgta tgttgtcatt attacggccc
     3001 gtgacgaagc cagatacctg cagggtacca tcgacagcgt cgctggacag acgcttctgc
     3061 cggttgaaat ggtgatcgtc aatgacggtt cccgggacga gaccggagcc atcgccgacg
     3121 cagccgccgc gaaatattcc tgggtgcacg tggtgcatcg tgaggaccgc ggcgaacgca
     3181 aggtaggcgg tggcgtggta gagacgtttt atgcggggta tgaggccctg cgcacgcagg
     3241 atttcgtcta ccagtgcaaa ctggacggtg atctcaccct cccgccgggc tatttcgagg
     3301 gtatcatccg gaagatggaa gctgatcccg cgctgggagg ggcgagcggc aaagtattca
     3361 atccggtcgg cggcggagcg gtcaaagaag aacgcattat cgacgatatg gtttccggtg
     3421 cggtcaactt ctggcggcgc gcctgctggg atcaaatcga cggctatgtg cgcatggtta
     3481 tgtgggacgg aatcgccatg caccgggccc ggatgttcgg atggcgcacg cgaagcttcc
     3541 gcgacaaaga tctcgagatc attcatcatc gcctcatggg ctcttcgcat aagaatatcc
     3601 ttcatggccg catgcgctgg ggacgtggac agtggttcat gggctgccat cccctgtata
     3661 tcctcgccag cggcatcaat cgcatgtttg aacgccccta tatgcttggc gggatctgca
     3721 tcgtggccgg atacttttcg agcatgctgc aaggcgcccc ccggtatgaa gacctcgagt
     3781 tccgcagaca cctgcatgcc tggcagctga aacgcctggg actgggcttt ctggcgcccc
     3841 acgtcactcc tcacgagcga agtacggcat gatgactcct ccggaacagt cttcatcacc
     3901 gtccagcgga atcttcttgt ctttcgtagt cattggatac aatgaggccg caacactgaa
     3961 agcctgcctg gagtcggtca aaaccgcgaa cctcgacgga ttttcgtggg agttgattta
     4021 tgtcgacgga ggatcgacgg atgacagcat ggagatcgcc cgcgaaacgg gcgtcaatct
     4081 cttgcttggc ggggagatgc ggcgccgtgc cgctgaaaat cgaaatctcg ggctcagcgc
     4141 tgcccgtggg cggttggttc aatttatcga cggagacatg acgctctcgc ccgactggcc
     4201 ccgtaccgcg gctcaatttt tgtctgagaa cgaggcagtt gcagcggtat gcggcaatct
     4261 ggaggaatcg gccccgggcg ttttttatgc agcccttcaa atcgactggg cacagcggga
     4321 aggtgaaatc cggcattgcg gcggcgctgc catgtatg
//
LOCUS       DAOPBQ010000307         4353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16593_length_4353_cov_4.905305, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000307 DAOPBQ010000000
VERSION     DAOPBQ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16593_length_4353_cov_4.905305"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKW60_10110"
     CDS             <1..470
                     /locus_tag="PKW60_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99827.1"
                     /translation="ASMQVTYEYGARGDLPPVRLTWYQGHEKPQILKDGGIPEWNSGV
                     LFVGEKGMLLADYGRHTLLPEASYADFTPPEPFIPASLGHHAEWLHACKTGAPTTCNF
                     EYAGWLTEANHLGNVAYRTGKKLEWDPVALQATNAPEAAQFIRREYREPWKLI"
     gene            536..658
                     /locus_tag="PKW60_10115"
     CDS             536..658
                     /locus_tag="PKW60_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99828.1"
                     /translation="MKKRHCLLIIALGSLFAAPAIAESLDQEFERLYAARNTCT"
     gene            749..1888
                     /locus_tag="PKW60_10120"
     CDS             749..1888
                     /locus_tag="PKW60_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HPJ99829.1"
                     /translation="MGDDASVRQASEAQTDASGRVVAVDVLRGFDMFWLVGGTGFGIA
                     VARLLGGNAGSFLESQFDHADWVGFTFYDLIFPLFAFLVGMSLVFSLEKRLQTGGRWA
                     AYKRLLRRAVLLFVMGLVYYGGFSKEWPDIRLLGVLQRLALCYLFAGILFIHLRPRGL
                     IIACLFCLIGYWLLLSFVPVPGTGQTSFDEHGNWAWYVDNQFLPGHKHYGDYDPEGLL
                     STIPAVATCLLGVFAAFLLRTAALTPWRKAVLFLAGGASLAALGYLWGLQFPVIKKIW
                     TSSYVLVAGGYSFMLLGFFYVLVDIFGFRAWARPFLWIGANPLTIYMARNMIDFNKLA
                     LRFVGGNVQLLAGEEWGYLLHTGCSLGLSLLVVWFLYRKKIFLRL"
     gene            1991..3565
                     /locus_tag="PKW60_10125"
     CDS             1991..3565
                     /locus_tag="PKW60_10125"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPJ99830.1"
                     /translation="MRMKGVAVFCLVTALALGGATAPALEPGQAAGPVRFENLDDVQR
                     TMTTENYASRKATVVVFLSGRSAAVLDQMEAMNALYDRVRLNGILFVGICPDPNQSGD
                     ELRTFLQHTGGIIPLYRDPQREAANQFGATVTPEYFMLDSKGELVYHGGLGQPGEGLD
                     RALQQHLSGEAVTAQAPLGGDPIGVEAPQRPVPDQYGTIYFASQLIFEKIPGAAVHHC
                     STVAEAPNGDILCLWYGGSYESSEDQALYLARQRRGAAAWEEPQRLLFDPSLPPGNAV
                     LFQGPDRKLWIIWGRMESDRPLRRGSGWGECRLMYRTSDDNGVTWSGDSEIPDGFGSL
                     PRNLPLTLADGRFAIPVSGEGRQAHGGSYLLFLDPAGPVWTRSGFARGGSQPTVIQRD
                     SGELLMLMRSSPRIRQSISPDGGTTWQPSEATELKNPDSGICMTRLKSGRIVLAFNDT
                     DGDDRTPFNLIQSDDGGVTWTNQRTLEADWGEFSYPSILQASDGRIHLTYTYRRFSIK
                     HTVFNENWLTTYERPN"
     gene            3624..>4353
                     /locus_tag="PKW60_10130"
     CDS             3624..>4353
                     /locus_tag="PKW60_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99831.1"
                     /translation="MKSLLSSAQEPGVPYFFLRIGLWGLCVCMALSACAAARAGEVRD
                     AVDWPAFLAASDPVWERWPENWWEAPYLGNGMMGTLIRKLDERTVEWQVGRSDVEDYR
                     PINYGMGRLPIGSFLLRTQGELTGCDMRLNLLNAEAGGLIQTSAGRIAFTTLVHADRM
                     AILVCWKGEGGEADSALEWHPKEALHPRLAVKPDPERKKTWEPNPGGYQSDCGGVPVW
                     VQPLTEGGYATAWLETGHDGWRALF"
BASE COUNT          758 a         1288 c         1431 g          876 t
ORIGIN      
        1 ccgcgtcgat gcaggtgact tacgaatatg gtgcgcgcgg cgatttgccg ccggtccggc
       61 tgacctggta ccagggccac gaaaaaccgc aaatcttgaa ggacggcggc attcctgaat
      121 ggaacagcgg ggttttgttc gttggtgaaa aaggcatgtt gctcgcggac tacggccgcc
      181 atacgctgct gcctgaagcg agctacgccg attttacgcc gcccgagccg ttcatccccg
      241 cgtcgctcgg acatcacgcc gagtggctgc atgcctgcaa gaccggcgcg ccgactacgt
      301 gcaacttcga gtatgcgggc tggctgaccg aggcgaacca tctgggcaat gtggcctatc
      361 gcaccggcaa gaagcttgag tgggatcctg tcgcacttca ggccaccaac gcgcccgaag
      421 ccgcccaatt tatccgtcgc gagtaccgcg agccctggaa gctgatctag acggcggact
      481 gaggagaatt tgtttctcag gctctataag cgcaagtaat aaggagggcc agagaatgaa
      541 gaagcgtcat tgccttctta tcattgctct cggttcactg tttgcggcgc cggcgatagc
      601 cgaaagcctc gaccaggagt tcgagcgctt gtacgcggcc cgcaatacgt gcacttagaa
      661 tttgaatgtg ggactgcaaa atgctcgacg tccccgggga ggcatctgct acaatacgcg
      721 ccattgtctc aacgggaggt tcactgcaat gggagatgac gcttcagttc gccaggcctc
      781 agaggcccag accgatgctt cgggacgggt cgtggccgtt gatgtgttgc gcgggttcga
      841 catgttctgg ctggtcggcg gaaccggatt cgggatcgcg gttgcccgtc tgctgggagg
      901 aaatgccggt tcgtttctgg aatcccagtt tgaccatgcg gattgggtcg ggtttacgtt
      961 ttacgacctc atttttccgc tgttcgcttt tctggtcggc atgtcgctgg tgttttcgct
     1021 ggagaaacga ctccagaccg ggggccgctg ggccgcctac aagcgcctgc tccgccgcgc
     1081 ggttctgctc tttgtcatgg ggctcgtata ttacggcgga ttcagcaaag aatggccgga
     1141 cattcgcctc ctgggcgtgt tgcagcgcct ggcgttgtgc tacctcttcg ccggcattct
     1201 tttcatccat cttcggccgc gcggcctgat catcgcctgc ctgttttgcc tgataggtta
     1261 ctggctgctt ctgtcgttcg tgccggtacc gggcaccggc cagacctcgt tcgacgaaca
     1321 cgggaactgg gcgtggtacg tggacaacca gttcctgccc ggccataagc attacggcga
     1381 ctacgacccc gaaggcctgc tcagcaccat ccccgccgtg gcgacgtgtc tgctgggtgt
     1441 gtttgcggcg tttctcctgc gcaccgccgc tctgactccc tggcgcaaag cggtgctctt
     1501 tcttgccggc ggcgccagcc tggccgcgct cggatatctt tggggactcc agtttccggt
     1561 gatcaagaag atatggacct cctcctacgt cctcgtggcg ggtggataca gcttcatgct
     1621 gctgggcttt ttttatgtgc tggtggacat cttcggattt cgcgcctggg cccggccatt
     1681 cctctggatc ggggcgaatc cgctcacgat ctacatggct cgaaacatga tcgacttcaa
     1741 caaactggcg ttgcggtttg ttggcggcaa cgtccagctc ctggccgggg aagaatgggg
     1801 gtatctgctc cacacgggct gttcattggg gctgtcgctg ctggtggtat ggtttctgta
     1861 ccggaaaaag atcttcctgc gcctgtaggc cccacaccgg ccacaacccg gagaatggca
     1921 cagcccgaat cctgtgtaat cccggccggt ggggcagcgt gcgggtatac gccggaaagg
     1981 aggcgcaagc atgcggatga agggagtggc tgtgttttgc ctggtgactg ctttggcgct
     2041 tggcggtgcg actgcgcctg cgcttgaacc cggccaggca gcggggccgg tccgtttcga
     2101 gaacctggac gacgttcaac ggaccatgac cacggagaat tacgcgtcgc gcaaagcgac
     2161 cgtcgtggtg ttcctttccg ggcgcagcgc ggccgtgctc gaccagatgg aagcgatgaa
     2221 cgcgctctac gaccgcgttc ggctcaacgg catccttttc gtgggcatct gcccggatcc
     2281 gaatcaatcc ggcgatgaac tgcgcacgtt tctgcagcat accggcggaa ttatcccact
     2341 ctatcgcgat ccgcagcggg aggccgcgaa tcagttcggc gccacggtga cccctgaata
     2401 tttcatgctg gacagcaaag gagaactcgt ctaccacggc gggctgggtc agccgggcga
     2461 aggccttgat cgcgcgcttc aacagcatct ttcgggagag gccgtgacgg cccaggcgcc
     2521 gctggggggc gatcccatcg gcgtggaggc gccgcagcgg cccgtccccg accagtacgg
     2581 cacgatctac tttgcgtcgc agctgatctt cgaaaagatc ccgggtgcgg ccgtgcatca
     2641 ctgctcgacg gtagcggagg cgccgaacgg agacatcctc tgcctgtggt acggcggcag
     2701 ctacgaatcc tcggaggatc aggcgctcta cctggcccgg cagcggcgcg gcgcggcggc
     2761 gtgggaagag ccgcagcggc tgctcttcga tcccagtctg ccgcccggca atgcggtgct
     2821 gtttcagggg ccggaccgta aactttggat catctggggg cggatggaat cggatcgccc
     2881 tcttcggcgc ggcagcggct ggggggagtg ccggctgatg taccgcacgt ctgatgacaa
     2941 cggcgtgacg tggagcggcg attccgagat cccggacggg tttggttcgc ttccgcggaa
     3001 tctcccgctg acgctcgccg acggccggtt cgccataccc gtgagcgggg aagggcggca
     3061 ggcgcatggc ggctcgtacc tgctgtttct ggacccggcg ggccccgtgt ggacgcgcag
     3121 cggattcgca cggggcggca gccagccgac cgtcattcaa cgcgattctg gagagctcct
     3181 gatgctgatg cggagcagtc cgcggattcg gcaaagcatc tcgccggatg gcggcacaac
     3241 ctggcagcca tccgaagcaa cggaactgaa aaatcccgac tccggcatct gcatgacccg
     3301 gctgaagagc gggcgtattg tgctcgcctt caacgatacc gatggcgacg accgcacgcc
     3361 cttcaatctc atccagtccg atgacggcgg cgtcacctgg acgaatcagc gcacgctcga
     3421 ggccgactgg ggagagttct cgtaccccag catcctgcag gcgtccgatg gccggattca
     3481 tctcacgtac acctaccggc gtttttcgat caagcacacc gtgttcaatg aaaactggct
     3541 gacgacctac gaacggccaa attgaacgag gcggattttt cgcgccacgg gcgggatgtg
     3601 ctggaagtga tgaggcgatc cctatgaaat ctctgctttc ttctgcacag gagcctggcg
     3661 tgccgtactt tttcctgcgc attggcctgt ggggcctgtg cgtgtgcatg gcgctcagtg
     3721 cgtgcgcggc cgcgcgggcc ggggaggtgc gggatgcggt ggactggccg gcgtttctgg
     3781 cggcttctga tcccgtgtgg gagcgctggc cggaaaactg gtgggaagcc ccgtacctgg
     3841 gcaatggcat gatgggcacg ctgatccgaa aactcgacga gcggaccgtc gagtggcagg
     3901 tgggccggag cgatgtcgag gattaccgcc cgatcaatta cggcatgggc cgcttgccca
     3961 ttggcagttt tctcttgcgg acgcaggggg aattgaccgg atgcgatatg cgcctgaacc
     4021 tgctcaacgc ggaagccggc gggctgatcc aaaccagcgc gggccgcatt gccttcacga
     4081 ccctggtgca cgccgaccgg atggcgatcc tcgtgtgctg gaaaggcgaa gggggtgaag
     4141 cggacagcgc gctggagtgg catccgaaag aagcattgca tccgcgcctg gcggtcaagc
     4201 ccgatcccga gcgcaagaag acctgggagc ccaatcccgg cgggtaccag agcgattgcg
     4261 gcggcgttcc ggtctgggtg cagcccctga ccgagggagg ctatgcaacg gcctggctcg
     4321 agaccgggca cgacggctgg cgggcactgt ttg
//
LOCUS       DAOPBQ010000308         4335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16677_length_4335_cov_3.796028, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000308 DAOPBQ010000000
VERSION     DAOPBQ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16677_length_4335_cov_3.796028"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PKW60_10135"
     CDS             complement(<1..576)
                     /locus_tag="PKW60_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HPJ99832.1"
                     /translation="MSEPSQNGDQNRTGLRLQRLLRLSAILVLFVALAAFGVTALLVN
                     IFERKQEARNPFFRVVELTDETEDPAIWGKNFPMQYDLYTRTVDMQRTRYGGSEAIPR
                     TPTSDDPRTVVAQSRLELDPRLKTMWAGYAFAIDFREERGHAYMLEDQMFTARQQKPQ
                     VGACIHCHASVYIPYKKLGNGDLIRGFELMNP"
     gene            complement(605..1060)
                     /gene="nrfH"
                     /locus_tag="PKW60_10140"
     CDS             complement(605..1060)
                     /gene="nrfH"
                     /locus_tag="PKW60_10140"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HPJ99833.1"
                     /translation="MRRRSIVFAVVLLSALAGIGMGVGSYTFIYAKGFSYMSNDAIIC
                     ANCHIMKNHFDAWAKGSHHAVAVCNDCHTPHSLIPKYFVKGLNGFNHSLAFTTGHFHE
                     PIQATAFNHRVAEANCRYCHEDIVHQIDVGGGREPQDCIRCHRYVGHWE"
     gene            1275..2078
                     /locus_tag="PKW60_10145"
     CDS             1275..2078
                     /locus_tag="PKW60_10145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPJ99834.1"
                     /translation="MTETFYDPWADYYDAIHTGLPGEAEFYIGQAVRMQAKTLELGCG
                     TGRIAIPMAMSGVDVVGIDNSKAMLRICREKKRRVGATSGRLRIVEADMREFDLGQLF
                     DLVILPYRAFMHLLTLECRASCLACVSGHLHPGGLLILNTWRPRECDLAAHRARERGA
                     LVFGGKYPLAGTGLDVAHYVSAWCDEENQILDEQHLLHEVDQRGEVLHSAILPMRRAW
                     ITPVQMQQLLQSQKFATEAVFGDFECGPFTTESTEMIWVAKKPGGGPAV"
     gene            complement(2097..2372)
                     /locus_tag="PKW60_10150"
     CDS             complement(2097..2372)
                     /locus_tag="PKW60_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPJ99835.1"
                     /translation="MTGRLRVHIQGRVQGVGFRYATYRQAVQRGLSGWVRNAVDGSVE
                     AEFEGDSDALQTMLDWCRQGPVFAVVTSVDEQWESGAPLYDGFQIRS"
     gene            complement(2372..3751)
                     /locus_tag="PKW60_10155"
     CDS             complement(2372..3751)
                     /locus_tag="PKW60_10155"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPJ99836.1"
                     /translation="MQKIIMFVFAIAIAVPALCQDIPITGVSGVNLREIAGTPTLVTV
                     VVKSRNAEDPNLQITDLGPDYVSVLNPETGDRHAYLFTDVKEIRVQGGKLEAKPFKQD
                     AERSLTPSQQQLVSRAIRQATEVFEEENRDQPTKMNAAMVIASQGDEEALKYLHELAR
                     QNDLETALGAYYRLYIAGNKTLDSELIERALTSGDRQIRGIAARVAGLYREQTAEPAL
                     LRMVKDRAADLSAPAALALARLGNREAIPTMLDMLTGLNEEKAKSAAKALVILKGDSV
                     VESLEEMLETQTGLARYRIIEMLYLLGAPKGKELLKQEALQIPTLRVEAALRLAPEGD
                     LDAMEALRERLSERYDIQLDVIRMRARAAGALIMGGDRTRIADLQELLRLDFPDLKEP
                     AAGNFVKGIASIVTEVVVETDVRPLMDIMQPVLLSPYPEARVSAASAIIALGDSALRA
                     RMLEQFQSN"
     gene            complement(3829..>4335)
                     /locus_tag="PKW60_10160"
     CDS             complement(3829..>4335)
                     /locus_tag="PKW60_10160"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99837.1"
                     /translation="DAILKKQLACARSRGEILHEAEQQILRLYDDATLAEIPELTRAR
                     NAVWYDETIVPLLQMLEEKQDRDPILCVRNDGALRDLPEDCSVEVPVRISKQGLEPRR
                     IGDCPRFLRGLFHSVKESDRLIVEAVRHKSYESALQALTINPLVPSLDAARGFLDRII
                     REESIELY"
BASE COUNT          825 a         1309 c         1256 g          945 t
ORIGIN      
        1 cggattcatc aactcgaagc cccggatgag gtcgccattc cccagcttct tataggggat
       61 atacaccgac gcgtgacagt ggatgcaggc gcccacttga ggcttctgct ggcgtgcagt
      121 aaacatctgg tcttccagca tgtacgcatg cccgcgctcc tcacggaagt caatggcgaa
      181 cgcatagccc gcccacatcg ttttcagccg cggatcgagt tccaggcggg actgcgcaac
      241 aactgtccgg gggtcgtccg aggtcggcgt ccgcggtatg gcttcactgc ctccataccg
      301 ggtccgctgc atgtccaccg tccgcgtgta caggtcgtat tgcatgggga agttcttgcc
      361 ccaaatcgcc ggatcttccg tctcgtccgt gagctcaacg acccggaaga aagggttgcg
      421 cgcttcctgc tttcgctcaa aaatatttac caggagcgcg gtcaccccga aggcggccag
      481 ggcgacaaac agcaccagga tggccgacag ccggagcagg cgttgcaggc gcagtccggt
      541 ccggttttgg tctccatttt gggatggctc gctcatggct ggtgctcctc attttccctt
      601 gatgctattc ccaatggccg acatagcggt ggcagcggat gcaatcttgt ggttcccggc
      661 cgccgccaac atcaatttga tgcacgatat cttcatggca ataccggcag ttcgcctccg
      721 caacccggtg gttgaacgcg gtcgcctgaa tcggttcatg gaagtgcccc gtcgtgaagg
      781 caagggaatg attaaatccg ttgagccctt tcacgaagta tttcgggatc agcgaatgtg
      841 gggtgtggca gtcgttacag accgccactg catgatgact gcctttggcc catgcgtcaa
      901 aatgattctt catgatgtgg cagtttgcac agattatcgc gtcgttgctc atatatgaga
      961 agcctttggc atagatgaag gtatatgacc ccacgcccat acctatccca gccaaggcgc
     1021 tcaacaggac aacggcgaaa acgatactcc gccgacgcat gctccctccc gttcgaatgg
     1081 ggaagatgat gtctggtgaa ttccagcctc tacccgcagg cacagtctag catattctgg
     1141 tccgcataaa caagagattt gcgataggat atgtcgcttt tgaggctggc cttagcgtgc
     1201 ccaatcccct atcatgcgcc caggcccaaa tgccggcccg acacggtctt ctttgaaagg
     1261 aatcgaagag cggaatgact gaaaccttct atgatccctg ggcggactat tacgacgcca
     1321 ttcatacggg actcccgggc gaggcggagt tctatatcgg ccaggccgtt cggatgcagg
     1381 ccaaaaccct cgagctgggg tgtgggacgg gccgaatcgc catccccatg gccatgtcgg
     1441 gcgtcgatgt agtggggatc gataattcga aggcgatgtt gcgcatttgc cgggagaaaa
     1501 agaggcgagt gggcgcgact tcggggcgcc tccgaattgt tgaggcggat atgcgtgaat
     1561 tcgacctcgg gcagcttttt gatctcgtga tattgcctta ccgtgcgttc atgcacctgc
     1621 ttacgctgga gtgccgtgcc tcgtgcctgg cgtgtgtctc tggccatttg cacccgggcg
     1681 gcctgctgat attgaacacc tggcggcccc gcgagtgcga ccttgccgcc caccgcgcgc
     1741 gtgaacgtgg cgcccttgtc tttggaggga agtatccttt ggcgggaacc gggctggatg
     1801 tagcgcatta tgtttcggcc tggtgcgatg aagaaaatca gattcttgac gagcagcatc
     1861 tgctccacga ggtcgaccag agaggagagg tgctgcactc cgccattctg cccatgcgcc
     1921 gtgcctggat cactcccgtt cagatgcaac aactccttca gagtcaaaag tttgcgacgg
     1981 aagcggtctt tggggatttc gaatgcggcc ccttcaccac ggagagtacc gagatgatct
     2041 gggttgcaaa gaagccgggt ggcggccctg ccgtgtaggc aaaaggctgc gccggttcag
     2101 cttcggatct gaaatccgtc atacagaggg gccccggact cccactgctc gtcaactgat
     2161 gtgactacag cgaagacggg gccctgacgg caccaatcaa gcatggtttg cagggcgtcc
     2221 gaatcgccct cgaactcagc ctccacgctt ccgtccacgg cattgcgtac ccagccggag
     2281 agacccctct gcaccgcctg gcggtacgtg gcgtagcgga atccgacgcc ctgtactctc
     2341 ccctggatat gtacgcgcag ccgcccggtc atcaattgga ctggaattgc tccagcatac
     2401 gcgcccgcag cgctgaatcg cccagggcga tgatggcgga ggctgcagaa acgcgggctt
     2461 ccggataggg gctcagcagg acgggctgca tgatgtccat gagcggccgg acgtctgtct
     2521 ccacaacgac ctcggtgaca attgatgcaa tccctttaac aaaatttcct gccgcgggct
     2581 ctttcagatc cgggaaatcc aggcgcagca gctcctgaag gtctgcaatg cgggtgcggt
     2641 cgccgcccat aatcaaggcg cccgcagctc tcgcccgcat ccgtatgacg tccagctgaa
     2701 tgtcgtagcg ctccgacaaa cgctcgcgca atgcctccat ggcatccagg tcgccctccg
     2761 gtgccaggcg cagggcagct tccacccgca gtgtcggaat ctgaagcgcc tcctgcttca
     2821 gcagctcttt gcctttcggg gcgccgagca ggtagagcat ctcgataatg cggtaccggg
     2881 cgagtccggt ctgggtttcc agcatctctt caagtgactc gaccacactg tcgcccttca
     2941 gaatcacgag ggctttggcg gcggatttgg ctttttcttc attgagcccc gtcagcatgt
     3001 cgagcatcgt gggaatcgcc tcacggttgc ccaaacgggc cagcgccaaa gccgccgggg
     3061 cggacaggtc ggctgcgcga tccttgacca tacggagcag tgccggttcc gccgtttgtt
     3121 cacggtacaa tccggcgact cgcgccgcaa tcccgcgaat ctggcggtcg ccgctggtga
     3181 gcgcccgttc gatcaactca ctgtccagcg tcttattgcc ggcaatatag aggcggtaat
     3241 aggctcccag ggccgtttcg agatcgttct ggcgcgccag ttcgtggaga tatttcaagg
     3301 cttcttcatc gccttgcgat gcaatgacca tggccgcgtt cattttggtc ggctgatcgc
     3361 ggttttcctc ttcaaacacc tccgtggcct ggcgaatggc acggctcacc agctgctgct
     3421 ggcttggggt caggctgcgt tctgcgtcct gcttgaatgg tttggcttcg agcttcccgc
     3481 cctgaacgcg gatttccttc acgtccgtga acagatacgc gtgccgatcg cccgtctccg
     3541 ggttcaggac gctcacatag tcggggccca ggtcagtgat ttgaagattg ggatcctcgg
     3601 cgttacggct tttgaccacg acggtaacga gcgtgggtgt tccggcgatc tccctgaggt
     3661 tcacgcccga gactcccgta atcggaatgt cctggcacag ggcgggaacc gcaatcgcta
     3721 tcgcgaaaac aaacatgatg atcttttgca tctttcgact actcccaatc gtggcggcgc
     3781 tgcattcctc tcaccgcgtt cctgcgtttc tcccggaagc cgttgaagct aatacaattc
     3841 tatactttct tccctgataa tgcgatccag aaatccgcgc gcggcatcca gcgacggcac
     3901 cagagggttg atggtcagcg cctggagggc cgattcgtac gacttgtgcc ggacggcctc
     3961 cacgatcagc cggtcacttt ccttaacgct gtggaacaac cctctcaaaa agcgcgggca
     4021 atcgccaatc cgccggggtt ccaggccctg cttgctgatc cgtaccggca cctctacact
     4081 gcagtcctcc ggaaggtccc ggagggcgcc gtcgttccgc acacagagaa tcgggtccct
     4141 gtcctgcttt tcttccagca tctgcaggag cgggactatc gtttcgtcat accagaccgc
     4201 gttgcgagcg cgtgtcaatt ccggaatctc cgccagcgtg gcatcgtcat acaatctcag
     4261 gatctgctgc tcggcctcgt gcagaatctc cccgcgcgac cgggcgcagg ccagctgctt
     4321 cttcaggatg gcgtc
//
LOCUS       DAOPBQ010000309         4333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16688_length_4333_cov_4.001403, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000309 DAOPBQ010000000
VERSION     DAOPBQ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16688_length_4333_cov_4.001403"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKW60_10165"
     CDS             <1..382
                     /locus_tag="PKW60_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPJ99838.1"
                     /translation="KMAGHDRYVIQEAMESEGERGAAALWQRISYINNVGVIAPLLGL
                     LGTVWGMMRAFGAIAFDDAQSRALSMAYSVSMAMITTAAGLLLAIPALLAYYYFRGRV
                     IKIIAEVEAQASEVVEALSRGEGI"
     gene            379..807
                     /locus_tag="PKW60_10170"
     CDS             379..807
                     /locus_tag="PKW60_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPJ99839.1"
                     /translation="MKFGNRASEELEGMQLAPLIDIVFLTLVFFMTTTVFTSMESELD
                     IQLPTASQAKMDERSQGEIYINLTSDGKVIVNDRELTLEQLQGVLNRVAELFPGGSVI
                     IRGDRNAVLGRAIAILNCCKNADIDNVSFAALQDEPAGAT"
     gene            816..3149
                     /locus_tag="PKW60_10175"
     CDS             816..3149
                     /locus_tag="PKW60_10175"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPJ99840.1"
                     /translation="MTNHRKFLLAACLLSMLSAAAAAQDPAQAQLDIANGFFRRGFFE
                     EAAAEYRAYIEGNPQAPQVDTALYRLGESEYAQRHYEQALTVYDQFLAKVPQGEESSR
                     ALLRKGEIHFHLNQFEQAESALNRVTGEETPAEVRAGALYYLGKLHFQKNDMPKSAAA
                     FATLSESFPDNPLAPFAQFQLAFVYLAQNALEKAASEFAEVASANTDDTLRAESQFRA
                     AETYDRLGWYEPAVAAYKKLQADFPNTPHAQKGAYGYAWALYHAGKAAEAIDAARAFV
                     SANPESPELGGMKYLEANCLQSLERYDEAIAVYKHIREAFPETEFAHRAHYKIAWSLF
                     LKGEEDAARAEIERFIDANPQSQFAGEASFLLASLLTKQQNYEDAFDLYRLIADKFSD
                     SEFGAEALFKQGEMLEALGRGPEAAAIFEQFAKTYPEHVLTEQAMLRIADKEFLQASF
                     GDAIEKYTQILQSATEPSVREEMLYRMAITYHNMQKYKESADTFAQVLEAFPQGPHVA
                     EAHLRIGDYLVREGKDPVKAIDSYSQALDTDPKGPYAGRALKGLALARYETKDYTTAT
                     ELFYRVITEFGEAALNEKTYLWTAQELHNAGKFDESARVLQALLTAMPNYASPNRVRF
                     KIAECRELASKTEEALQLYQAIVAEAPKSTEAVESWFRMGKLYETLGQPEKAFDMYQA
                     AANTNTGDTAARARFRIGEIQESKGEFEAAARSYMRVAILFLHEELSPESLWRAGQCY
                     EKANALEQARKAYNETVEEFPDSGQAAKARERLAAMG"
     gene            3165..>4333
                     /locus_tag="PKW60_10180"
     CDS             3165..>4333
                     /locus_tag="PKW60_10180"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99841.1"
                     /translation="MLRRRTLLLLSIAVSVLLHAALIGLAPRVPMLRVAAPAPGIAER
                     FRVRVTEDLPPVTVEPEPDPTRDLTTRSGSVEELLKREREELRPAESLIEQDVSVPNL
                     VQRVSTEPLTRTHDLQFDEAALKKVDARIIEIARETARQDIQIARRLVRPSPMRILGE
                     DEFPVMRTEGADLPEELLTLDPLNPGGGLGGTGDEPPGLAGELPEREQIPAPAQALDT
                     PEKILASLPDERLTGPQTEVTRATEEARKKYEFLDDLVDIEIATYRREGDPQGYFRLN
                     IVPKAGESIEVLPKDVTFVIDASGSIAQLALDNTVKGLRQALSRLRPEDRFNLIVFRD
                     TASGLERGFVEATPEEKEAALSFVRDLRSFGKTDVYSAIGPVVERAPRDGVPGVV"
BASE COUNT          949 a         1296 c         1273 g          815 t
ORIGIN      
        1 gaagatggcg ggacacgatc gctatgtcat ccaggaggcc atggaaagtg aaggcgagcg
       61 tggggccgcg gcgctgtggc agcgcatttc ctacatcaac aacgtcggtg tgatcgcccc
      121 gctgcttggc ctgctgggga cagtgtgggg catgatgcgg gccttcggcg ccattgcctt
      181 tgatgacgcc cagagccggg ccctgagcat ggcctacagc gtctcgatgg ccatgatcac
      241 cacggcggcg ggccttttac tggccattcc cgccctgctg gcctactact atttccgggg
      301 acgggtcatc aaaatcatcg ctgaggtcga agctcaggcg agtgaagtcg tcgaagcgct
      361 gtcacgggga gaaggcatat gaaattcggc aatcgcgcca gtgaagagct cgaaggtatg
      421 cagctagcgc cgctgatcga tatcgtcttt ctcacgctcg tgttcttcat gaccaccacg
      481 gtattcacct cgatggaaag cgaattggat attcaactcc ccaccgcaag ccaggcgaaa
      541 atggacgaac ggtcgcaggg agaaatctat atcaacctga ccagcgatgg taaggtgatc
      601 gtcaatgacc gcgaactgac cctcgaacag ttgcagggtg tactcaaccg cgttgcggaa
      661 ctcttcccgg gcggttccgt cattatccgc ggcgatcgca acgccgtgct ggggcgtgcc
      721 atcgccatcc tcaactgctg taagaacgct gatatcgaca atgtctcctt tgccgcgctg
      781 caggacgagc cggcgggggc aacatgagtg catccatgac gaaccaccga aaattcttgc
      841 tggctgcgtg tctgctgagc atgctgagcg ccgcagctgc cgcccaggac cctgcgcagg
      901 ctcagctcga tatcgcaaac ggttttttcc gccggggttt ttttgaagaa gccgccgcgg
      961 aataccgcgc ctatatcgaa ggcaatcctc aggctccgca agtggacacc gccctgtacc
     1021 ggctggggga atctgagtac gcgcagcggc actatgagca agccttgacc gtctacgacc
     1081 agtttctggc gaaggttccg caaggagagg aatccagccg tgcactgctg cgcaagggcg
     1141 aaatccactt ccatctgaac cagttcgagc aggcggaatc ggccttgaac cgcgtgacgg
     1201 gggaggagac gcctgcggaa gtccgggcgg gggccctgta ttatctcgga aaactccact
     1261 tccagaaaaa cgatatgccg aaatcggccg cggcgttcgc aacgctttcg gagagttttc
     1321 ctgacaaccc gctggcgcct ttcgcgcaat ttcaactggc atttgtctac cttgcccaga
     1381 atgcgctgga aaaggccgct tcggaatttg cggaagtcgc gtcggccaac accgacgaca
     1441 cacttcgcgc cgagagtcag tttcgtgccg cggaaaccta cgatcggctg ggctggtatg
     1501 aacctgccgt cgcggcctac aaaaaactcc aggccgactt tcccaatacg ccccatgccc
     1561 agaaaggagc gtatggctat gcatgggcct tgtaccatgc aggcaaggcg gcagaggcca
     1621 ttgacgcggc gcgggctttt gtcagcgcca acccggaatc gccggagctg ggcggtatga
     1681 aatatctcga agccaattgc ctccagtctc tcgaaaggta cgatgaagcc attgccgtct
     1741 acaagcacat ccgcgaggcc tttccggaga cggaatttgc acatcgtgcc cactacaaga
     1801 ttgcctggtc cctgttcctt aagggcgaag aagatgctgc ccgcgccgaa atcgagcgct
     1861 tcatcgacgc caatccccag tcccaattcg cgggcgaagc ctcttttctg ctggccagtc
     1921 tgctgacgaa gcaacagaat tatgaagacg ccttcgactt gtaccgtctg attgctgaca
     1981 agttcagcga cagtgaattc ggcgccgaag ccttgttcaa gcagggggaa atgctggagg
     2041 cccttggccg cggcccggaa gccgctgcca tttttgagca gtttgcgaag acatatcccg
     2101 aacatgtgct gaccgaacag gccatgctcc ggatcgctga caaggaattt ctgcaagcct
     2161 cgttcgggga tgccatcgaa aaatacacgc aaatcctcca gtcggctacc gagccttcgg
     2221 tgcgggagga gatgctctac cgtatggcca tcacctacca caacatgcag aaatacaagg
     2281 aaagcgccga cacctttgcg caggtactcg aggcatttcc gcagggaccg cacgtggccg
     2341 aagcgcatct gcgcattggc gactatcttg tccgtgaagg caaagacccc gtgaaagcga
     2401 tcgattccta ctctcaggcc ttagacaccg atcccaaagg accttatgcc ggccgcgcat
     2461 tgaaagggct tgcccttgcc cggtacgaga ccaaggacta tacgaccgca acggagttgt
     2521 tttaccgggt aatcaccgaa ttcggcgaag cggcattgaa tgaaaaaacc tatctgtgga
     2581 cggcccagga gttgcataac gccggcaaat ttgatgaatc ggcccgagta cttcaggctc
     2641 tcctgacggc catgcccaat tacgccagtc cgaaccgggt ccgcttcaag atcgccgagt
     2701 gccgcgagct cgcgtccaag accgaggagg cgctgcaact ctatcaggcg attgttgctg
     2761 aagctccgaa gagcaccgag gcggtcgaat cctggttccg catgggcaag ctttacgaaa
     2821 ccctaggcca gcccgaaaaa gcgtttgaca tgtatcaggc ggcggcgaac accaataccg
     2881 gagacacggc ggcccgggcg cggtttcgca ttggcgagat tcaggaaagc aagggcgagt
     2941 tcgaggctgc cgcgcgcagc tacatgcgcg tcgccatcct gttcctccac gaagagctgt
     3001 ctccggaatc gctctggcgg gccgggcagt gttatgaaaa agccaacgcg ctcgagcagg
     3061 cgcgaaaagc atacaacgaa acggttgagg aattccctga ctctggccaa gcggccaagg
     3121 cccgggaacg cctcgctgcc atgggataaa ccagactcct actcatgctg cgacgaagga
     3181 cattgctgct gctcagcatc gcggtctccg tgctcctgca cgcggcgctg atcggattgg
     3241 cgccgcgcgt gccgatgctt cgtgttgccg cgcccgcacc gggtatcgca gagcgcttcc
     3301 gggtgcgtgt gacggaagac cttcctccgg tcaccgtgga gccggagcct gaccccaccc
     3361 gggatctgac tacccggtcc gggtcggtgg aagaacttct caaacgcgaa agagaagagc
     3421 tgcggccggc cgaatccctg atcgaacagg atgtgagcgt gcccaatctg gtccagcgcg
     3481 tctccacgga accgttgacg cgaacgcacg acctgcagtt tgatgaagcc gcattgaaaa
     3541 aagttgacgc ccgcattatc gaaatcgcac gcgagacagc gcgccaggac attcaaattg
     3601 cccgccgtct tgtccggccc agccccatgc gtattctggg tgaagatgag tttcccgtga
     3661 tgcgcaccga gggcgccgat ctgcctgaag aactcctgac tttggatccc ctgaacccgg
     3721 gcggcggatt gggcggcacg ggcgatgaac cacccggcct cgccggcgag ctgcccgagc
     3781 gcgagcagat tccggcacct gcgcaagcac tggacacccc cgaaaaaatc ctggcctcac
     3841 tgcccgatga acggttgacc gggccacaga ccgaggtcac ccgggctacc gaagaagcca
     3901 ggaaaaaata tgagttcctc gacgatctgg tcgatatcga gatcgccacc tatcggcgtg
     3961 aaggcgatcc acagggctat ttccgcctga atatcgtccc aaaagccggc gaatccatcg
     4021 aagtcctgcc caaagacgtc acgtttgtta ttgacgcatc aggcagtatc gcccaactcg
     4081 cgctggacaa taccgtcaag ggtctgcggc aggcgctgag ccgtctacgc cccgaagacc
     4141 gttttaatct tattgtgttc cgagacactg cgtccggcct cgaacgggga tttgtggaag
     4201 ccacgccgga agagaaggag gcggcgctga gctttgtgcg cgatctccgt tcgtttggaa
     4261 agacggatgt ttacagcgcc atcgggcccg tggtcgaacg ggcgccgcgc gacggcgtcc
     4321 ccggagtcgt ctt
//
LOCUS       DAOPBQ010000310         4305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16832_length_4305_cov_3.667059, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000310 DAOPBQ010000000
VERSION     DAOPBQ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16832_length_4305_cov_3.667059"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PKW60_10185"
     CDS             <1..318
                     /locus_tag="PKW60_10185"
                     /inference="COORDINATES: protein motif:HMM:NF037014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99842.1"
                     /translation="DVLYTNGEPIARHGEIGGRRRSEPHIRSLIRDTARGEDLFQTCF
                     EQGTRLLASPPLPAIRERALRCIAALPDEFKRLRNPEIYRVMLSEAVGREKAALLENP
                     DMA"
     gene            401..2326
                     /gene="nadE"
                     /locus_tag="PKW60_10190"
     CDS             401..2326
                     /gene="nadE"
                     /locus_tag="PKW60_10190"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPJ99843.1"
                     /translation="MRLVKIGVASISVNVGDFFGNSARVREVFDAARAEGVHLLVTPE
                     LGLSGYSLEDRVFWPGIARRSWAELESLASDTAGLAAFVGLPVCMDSMMYNGAALVYD
                     GAVRGVILKKYLPTYSIFYEGRNWASWRRGVTEIHGVPAGELVFALPFGKVSAEICED
                     LWTAKEPARARVHAGAEIICNSSASPFTPLKNEQRRRLVLGAAGSLACVYAYANLLGC
                     DSGRLVFDGGGFIATPDGIAAEGPLLSKDYWTLTTGVVDLDDIARVRTENSTWRQNAE
                     QPPQPGGILTVDLSAGAYTPARVEEFTAQLPKNLFVPACPQRSPATDEFLDQLFDALV
                     LGLRDYFEKAGVFEKFLVALSGGRDSALCLILAVEAAKALNAGADAANYAQRIKTAYL
                     PHSAHSSDATQSAARALAEAFRVPFMVAGIDDECAIALRLAGELAGGEERVTPVARQN
                     LQARIRGSMMLNWANNERGLVLVSSNLSEAAVGYTTTGGDNQGGYAPIANVPKTLVTA
                     LLERIAARDNLAALTKVLALPPSAELAPNQTDEADLMPYVVLDDLLFLFARKRMSLAD
                     CWRILVVRHPDCPREQLRSWTAHFGNLFVHNQWKREQSPVALKVLELDLDPKTGFRFP
                     VTQSIDAELQELASAEL"
     gene            complement(2323..2862)
                     /locus_tag="PKW60_10195"
     CDS             complement(2323..2862)
                     /locus_tag="PKW60_10195"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPJ99844.1"
                     /translation="MPILYYIDGYNVIHHCDRLRALARVDFEAARDALVDRVTRYCCD
                     AGQAARIVFDGRGRSSDRAAPVLSSPCIEVLYSSEQQTADALIERMVYETGKRTELVV
                     VTSDRGIRDLCGGLGALTMHPAHFLETIEESLRQARSSLQAAQYRVQRATRMEDHLDD
                     ETRASLESLRQQLADRENH"
     gene            complement(2894..4015)
                     /gene="alr"
                     /locus_tag="PKW60_10200"
     CDS             complement(2894..4015)
                     /gene="alr"
                     /locus_tag="PKW60_10200"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPJ99845.1"
                     /translation="MDSTAQSTFVVDLAAYAQNLRVVREMIPRECAIMAVVKGNAYGH
                     GSVAIAQRALQTGAAMLGVATVEEAIVLRSASITAPIMVLMQPPAEAFPEAIENNLRL
                     MLSDVDAVEKLGELARKRNRVIPIHCKVDTGMGREGLDPESAIAAMRHATRVSHIDIE
                     GIATHFSSADDIDEPATANQIRVFRQVLRQFEKEGIPYEMAHAANSAGIVGHPASAFN
                     MVRPGLMTYGVWPFDTPPERMALRPVGRWESKIVLIKELPSGWSIGYGRTYITDRPTR
                     TAVIPVGYADGYKVQFGNNADVLIRGRRCPVRGRVSMDQIVVDVTEVPGVTTGDPAVL
                     IGVDGPEQISVAELAKRARTVGQDILSCIGPRVHRVYVN"
     gene            complement(4017..>4305)
                     /locus_tag="PKW60_10205"
     CDS             complement(4017..>4305)
                     /locus_tag="PKW60_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99846.1"
                     /translation="LVTGVRSLAASEGVIIAANKYGKTKAVLQMVFVHVFLGLIILKR
                     LTALWFPDPVAEYAWLLHYASLCTIIFVAVYTVYSGVRYAQLNWQTLHLGS"
BASE COUNT          775 a         1308 c         1406 g          816 t
ORIGIN      
        1 gacgtgctgt acacgaacgg cgaacccatt gcacggcacg gcgaaatcgg cggccggcgc
       61 cgcagcgagc cccatatccg gtcgctgatc cgcgacacgg cccggggcga agaccttttc
      121 cagacgtgct ttgaacaagg cacgcgcctg ctggcgtccc cgccgctgcc ggctattcgc
      181 gagcgggcct tgcggtgcat cgctgctctg ccggatgagt ttaagcggct ccggaacccc
      241 gagatttacc gggtcatgct ctccgaagcg gtcggccggg agaaggccgc gctcctcgaa
      301 aacccggata tggcgtagga ggatggctga tgctggcgtt ggacatgcag cagagaggcg
      361 cgtcattcag ccgcaacggc tgcaggggcg agggggtcgt atgcgtctag ttaaaattgg
      421 cgtagcgtcg atatcggtta acgtgggcga ctttttcggc aacagcgcgc gcgtgcgcga
      481 agtgtttgat gctgcgcggg cggaaggggt gcacctcctg gtgacaccgg agctgggcct
      541 gtcgggctac agtctcgagg atcgggtgtt ctggcctggc atcgcgcgcc gcagctgggc
      601 agagctggaa tcgcttgcct ccgacactgc cgggctggcc gcatttgtcg ggctaccggt
      661 ctgcatggat tccatgatgt acaacggcgc ggccctggta tacgacgggg cggtgcgcgg
      721 cgtgatcctc aagaagtacc tgcctaccta cagcatcttc tatgaaggcc gcaactgggc
      781 ttcgtggcgc cggggcgtga cggagattca cggtgtgcct gcgggcgaac tcgttttcgc
      841 gctcccgttt ggcaaggtca gcgccgagat ctgcgaagat ctgtggaccg ccaaggagcc
      901 ggcccgcgca cgcgtgcacg caggcgcgga gatcatctgc aacagcagcg cttcgccgtt
      961 tactccgctc aagaacgagc agcggcggcg cctggtactg ggcgccgcgg gcagtctcgc
     1021 gtgcgtgtat gcctacgcca acctgctcgg ttgcgacagc ggccggctgg tgtttgacgg
     1081 gggaggtttt atcgccacgc cggacggcat tgcagctgaa ggaccgctgc tgtcgaaaga
     1141 ctactggacc ctcacgaccg gggtggtcga tctcgatgac atcgcacgcg ttcgtacgga
     1201 gaattcgacg tggcgccaaa acgcggaaca acctccccag ccgggcggca ttctgaccgt
     1261 ggatctatct gcgggtgctt acactcccgc gcgcgttgaa gaattcacgg cgcagctgcc
     1321 caaaaatctg tttgtgccgg cgtgtcccca acgttcgccg gcaacggacg aatttctgga
     1381 ccagttgttt gatgcgctgg tgctcggctt gcgcgattat tttgaaaagg cgggtgtttt
     1441 tgaaaagttt ctggtggccc tgtcgggcgg gcgcgacagc gcgctctgcc tcatcctggc
     1501 cgtggaggct gccaaagcct tgaacgccgg cgcggatgct gcgaactacg ctcaacggat
     1561 caagacggcc tatctcccgc attccgcgca cagcagcgat gcgacgcagt cggcggcccg
     1621 cgcgctggcc gaggcgttca gggtaccgtt tatggtcgcg gggatcgacg acgaatgcgc
     1681 gatagctctc agactggcag gggagctggc gggcggcgag gagcgggtca ctccggtggc
     1741 gcggcaaaat cttcaggcgc gcatccgcgg aagcatgatg ctcaactggg ccaataacga
     1801 acgcggactt gtgctggtct cgtccaatct gagcgaagcc gccgtcggct acaccacgac
     1861 gggcggcgac aatcaaggcg gttacgcgcc gatcgccaat gtccccaaga cgctggtcac
     1921 ggcattgctg gagaggatcg ccgcgcggga caatctcgcc gcgctcacga aggtgctggc
     1981 gcttcctcca agtgcggagc tggcgccgaa ccagaccgat gaggccgatc tcatgccgta
     2041 cgttgtgctg gacgatctcc tgtttctatt tgcccggaaa cgcatgagcc ttgccgattg
     2101 ctggcgcatt ctggtggtcc gccacccaga ctgcccccgg gaacagttgc gctcctggac
     2161 ggcccatttt gggaacttgt ttgtgcacaa tcagtggaaa cgcgagcaat cgcccgtagc
     2221 gctcaaagtc ctggagcttg atctggaccc caaaacgggg tttcgcttcc cggtcacgca
     2281 gagcatcgac gcagaactcc aggagctggc atcggcagag ctctagtggt tttcgcggtc
     2341 agcgagctgc tgccggaggc tttccaggct ggcgcgggtt tcgtcgtcga gatgatcttc
     2401 catgcgcgtg gcacgctgga cccggtactg tgctgcctgg aggctgctgc gggcctgccg
     2461 caggctttct tcgatggtct caaggaaatg cgcggggtgc atggtgagcg cgccgagccc
     2521 gccgcacaag tcgcggatcc cccggtcgct ggtaacgacg acgagctccg ttcgcttgcc
     2581 ggtttcgtac accatgcgtt cgatgagtgc atctgccgtc tgctgctccg aagaatagag
     2641 aacctcgatg cacggcgacg acagcaccgg agcggcccgg tccgagctgc ggccgcggcc
     2701 gtcaaagaca atcctggcgg cctggccggc atcgcaacag tagcgggtta cgcggtcgac
     2761 cagcgcgtcg cgggccgctt cgaaatccac acgggcgagc gccctgaggc ggtcgcagtg
     2821 gtggatgacg ttgtacccgt ctatgtaata cagaatgggc atatcagcgg accctcgccc
     2881 ggggtcgcgt tgctcagttc acgtacacgc gatgcactcg cgggccgatg cacgacagga
     2941 tgtcctggcc aacggttctg gcgcgtttgg ccagttccgc cacgctgatc tgttccggac
     3001 catccacacc gataagaacc gcgggatctc cggtcgtcac gccgggcact tcggtcacgt
     3061 cgaccacaat ctggtccatt gaaacccggc cgcgaactgg gcatcgccgt ccgcggatga
     3121 gcacgtcggc gttgttgccg aattgcacct tatagccgtc ggcatagccg acagggatca
     3181 cggcggtgcg tgtcgggcgg tcggtaatgt aggtgcggcc gtagccaatg ctccagccgg
     3241 acgggagttc tttgatgagg acgatcttcg attcccagcg cccgacggga cgcaaggcca
     3301 tgcgctcggg aggggtgtcg aagggccaga cgccataggt catcaggccg gggcgcacca
     3361 tgttgaaggc gctggccgga tggccgacga taccggcgct gttggcggcg tgcgccattt
     3421 cgtaggggat gccttccttt tcgaattgcc tcaagacctg ccggaatacg cggatctggt
     3481 tggcggtcgc cggttcgtca atatcatcgg cgcttgaaaa gtgcgtggcg atgccttcga
     3541 tgtcgatatg agagacccgc gtcgcgtgac gcatggccgc gatggcgctt tcagggtcca
     3601 ggccctcacg gcccattccg gtgtcgacct tgcaatgaat ggggatgacc cggttgcgtt
     3661 tgcgcgccag ctcacccagc ttttccaccg catcgacatc tgacagcatc aaccgcaggt
     3721 tattttcgat cgcctcagga aaggcctccg cgggcggctg cataaggacc atgatgggtg
     3781 cggtaatgct tgctgagcgc agcacgatcg cctcttccac ggtggcaacg ccaagcattg
     3841 ccgccccggt ctgcagggcg cgctgcgcga tggccacgga gccgtgacca taggcattgc
     3901 ccttcacgac ggccatgatg gcgcattccc tggggatcat ctcgcgaacc acgcgcaggt
     3961 tctgcgcata ggccgccaga tcgacaacaa atgtagattg tgccgtgctg tccatctcaa
     4021 ctccccaggt gcaacgtctg ccagttcaat tgcgcatagc gcacaccgga atagacggtg
     4081 tacacggcca cgaaaatgat cgtgcacaac gaggcgtagt gcaggagcca cgcatattcc
     4141 gccaccgggt ccggaaacca cagggcggtc agacgcttca gtatgatcaa gcccagaaac
     4201 acgtgcacga agaccatctg aagcaccgcc ttggtcttgc catatttgtt ggcggcaatg
     4261 atcacacctt cgctggccgc cagcgatcgc actccggtga ccagg
//
LOCUS       DAOPBQ010000311         4302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16846_length_4302_cov_4.635743, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000311 DAOPBQ010000000
VERSION     DAOPBQ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16846_length_4302_cov_4.635743"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PKW60_10210"
     CDS             complement(<1..607)
                     /locus_tag="PKW60_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99847.1"
                     /translation="MTCRTHERRKGSHTRFAIPLFLALALLFRTAFAGEPPVLENAGL
                     RVAFDAETGRLVEFTDTLNGHAFIDVAATDDLWSLHMLPSQPVTEIRPGDAQSFTYAS
                     PDGNSAVLKLIWHNFALTEAPQLQVIVTVSLGPAAPIAYWSIAVENTADLALASLRFP
                     RISGIAPQENERLAVPSWMGELAAEPRRFLSRPDAPRRIEWE"
     gene            1093..2019
                     /locus_tag="PKW60_10215"
     CDS             1093..2019
                     /locus_tag="PKW60_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99848.1"
                     /translation="MGVDFEARMYQMFGAPEPLKDETVMLEAIDSKGQVSQVPLVIQT
                     RQLRSLTDANVAMKAMRLVGEDEFITASYKEVPEPVEAETESLFCRVYEEAAAQAIAM
                     EARLIVMLKGGEWTVPEGKKPVANAIAIRGLDFEKGQKKNWNKTYDDTMYLVLESASA
                     PPEVFEYRMTTESSSESKGVGRLESKQVTYIRGKHRGTDPAYRLQGNAAPGTRLGLEG
                     EQQITGANIHSAYSKQPIDSETPLQPNVSLGCQVVAASKRDFEKALVQALDKRGVTQF
                     LYTIVDDAELVEFNRILESRGVTPILADAVSR"
     gene            complement(2038..3336)
                     /locus_tag="PKW60_10220"
     CDS             complement(2038..3336)
                     /locus_tag="PKW60_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99849.1"
                     /translation="MPSARRRTPFTILAAAILCVLSVSPAVAQETIPAPGEPVTPNAY
                     QGSDSERINQAVAAAAASGRRVVIPRTNYAPDGPRDLWLLDSAILVQSGTTLELENCR
                     IKLSDRCRDNFIRSANCGLGITDIAPISNVSIRGIGAAVLEGADHPRATGDSAKILGE
                     RTYGTDANVADESQSGDWRNIGILLAFVEQFSIENIRLVDSHAWAISLERCAYGIIRD
                     IDFASTGYKTIDGKKETILNQDGLDLRQGCHDILIENLTGYTGDDLLALTNILRADAT
                     AGTIESTMVSAAVNRGDGADDIRNITIRNLRGYCAGGHHIVRFLNASGLRIHDVILDG
                     LIDTSGSARPCRAAIKIGDSNPRWGGVTPLGDTYRFFISNIMSRSQHTVLIAGSLSES
                     IVSNVIKYEAEGPPITMESGEDNVRNVIFSNVREMTPPGE"
     gene            complement(3423..>4302)
                     /locus_tag="PKW60_10225"
     CDS             complement(3423..>4302)
                     /locus_tag="PKW60_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99850.1"
                     /translation="GPARVGLQIHAQPGPWTAPGPSWDAADAVTLETFDDLAPYLPGA
                     APGVAAIAHGEAGSTLEGVTQQLALSTDGPGEGGSCAVYSAESELSTDTGWTVIGKPF
                     AQPLDLSDHRAIGLWLRGDGNGGSFKLQLHDGARAADFYIANNYTGWRYHQLRRPETD
                     PIDYSQVRRLNFYYNGLPARTAVSCAIGEIRALPALDVRTLTDPWVEIGGRRIECPAT
                     LHDGQYAILWPGEPLRQYGLPLDEPALSGAAIETFELPEGEYTVNFGCTGPVMAPLRA
                     RITLQPPEQHAMPSAR"
BASE COUNT          895 a         1210 c         1391 g          806 t
ORIGIN      
        1 attcccattc gattcggcgg ggagcatccg ggcgggagag aaatcgccgc ggttcggcag
       61 ccagctcacc catccagctg ggcacggcaa gccgttcgtt ttcttgcggc gcgatgcccg
      121 aaatccgggg aaaacggagc gaggcgagcg ccaaatcggc cgtgttttcc accgcgatgc
      181 tccaatacgc gatcggggcg gccggaccca gcgagacagt cacgatcacc tgcaattgag
      241 gcgcctctgt cagggcaaaa ttgtgccata taagcttcag gactgcgctg ttgccgtcag
      301 gagaggcata agtaaaggat tgggcgtctc ccggcctgat ctcggttaca ggctgggacg
      361 gcagcatgtg gagagaccac aggtcatccg ttgcggcaac gtcgatgaag gcgtggccgt
      421 tcaaggtatc cgtaaattcc acgaggcgcc cggtttccgc gtcgaatgca actcgcagcc
      481 cggcgttttc caatacgggt ggctcgccgg caaacgccgt ccggaacaat aaagccaagg
      541 ccagaaacag gggaattgca aaccgggtat ggctgccttt gcgccgttcg tgtgtgcgac
      601 aggtcatttg aagcgatctc cctggcgttt catgaaagcc gcagaccgag tctatcagat
      661 gccaggaagg taaaaaagaa gacgcaaaac ggcacaattg ctgcgtccgc ggcggattgt
      721 tggccagcgt actggcaccg gggtggtatt attagaaata aagaaataag tattggctgc
      781 tggcggcaca ggagaaccgg tatgtcgacg ccccgcgtgt attcttgcct cactgcactg
      841 gtcatgctct tattgctcct tccggaatgc gcaacggctc caaaacaagc acccaaacct
      901 tcaccggaaa agaagggctt atccggcgtt acatcgcgga tctcggacat cctgcgcgag
      961 atgagcgaga tacgcagagc tgaatcctcg gatgatgaaa catatcccga cacggcagcg
     1021 tccggccaga gtcgcctcga gcagagactg aacaggcagg cccaggtcct tgccagggcg
     1081 gcgagccgcc agatgggggt tgatttcgag gctcggatgt atcagatgtt tggcgctccg
     1141 gaaccgctca aagatgagac agtgatgctg gaggccatcg acagcaaagg ccaggtctcc
     1201 caggtgcccc tggtgataca gacgcggcag ttgcggtcac tgacggacgc gaacgtcgcg
     1261 atgaaggcga tgcggctggt gggggaggac gagtttatca cggcctcata caaggaagtg
     1321 ccggaacctg tggaggcgga aacggagagt ctgttttgcc gggtctacga agaggcagcc
     1381 gcgcaggcca ttgcgatgga ggcccggctg atcgtcatgt tgaaaggcgg cgaatggacg
     1441 gtacccgaag gcaagaaacc cgtggcgaac gcgatcgcga tacgaggcct ggattttgaa
     1501 aaaggacaga aaaaaaactg gaacaaaacc tacgacgaca ccatgtacct cgtgctggaa
     1561 agcgcgtcag ctccgcccga agtgtttgaa taccgcatga cgacagagtc gagcagtgag
     1621 agcaaaggcg tggggcgcct cgaatcaaaa caggtgacct atattcgcgg taaacatcgc
     1681 ggcaccgatc ccgcctatcg gctccagggc aatgcggcgc ccggcacgcg attgggactc
     1741 gagggcgaac agcagattac gggagccaac atccattccg cctactcaaa acagccgatt
     1801 gacagcgaga cgcccctcca gccgaatgtg tcgctgggct gccaggtggt ggcggcgagc
     1861 aagcgggatt tcgagaaagc actcgtgcag gcgctcgata aacggggagt cacacaattc
     1921 ctctacacca tcgtggatga tgcggaactg gttgagttca accggatcct ggagagccgc
     1981 ggcgtgacac ccatactggc agacgccgtc tcgcgctgag cgccggaata tcccagccta
     2041 ttccccgggc ggcgtcattt cccggacgtt gctgaagatg acgttacgga cgttgtcttc
     2101 accggattcc atggtgatgg gtggcccttc tgcttcatat ttgatcacgt tggagacgat
     2161 ggattcagac agtgagccgg cgatcagcac cgtgtgctgg gaccggctca tgatgttgct
     2221 gataaaaaac cggtaggtat cgcccagagg ggtaacacca ccccagcgcg gattgctgtc
     2281 gccgattttg atcgcagcac ggcagggccg cgcggaaccg gaggtgtcga tcaggccgtc
     2341 caggatgaca tcgtgaatcc ggagtccgct ggcgttgagg aaacgcacga tgtgatggcc
     2401 tccagcgcag tatccgcgaa ggtttcgaat ggtgatgttg cggatgtcat ccgcgccgtc
     2461 tccgcggttg acagccgcgc tgaccatggt cgactcgatc gtaccggcag tggcgtcagc
     2521 ccggaggatg ttcgtgagcg cgaggaggtc gtcgcccgtg tatcccgtga gattttcgat
     2581 gagaatatcg tgacatccct ggcgcagatc gagcccgtcc tggttgagaa tggtctcttt
     2641 cttgccatcg atggtcttgt atcctgtgga ggcaaagtcg atatcgcgga ttattccata
     2701 tgcgcagcgt tcgagggaga tcgcccaggc gtgagaatcg acaagccgga tgttttcgat
     2761 gctgaactgt tcgacaaagg ccagcaggat gccgatgttg cgccagtcgc cggactggct
     2821 ttcatctgcg acgttggcgt cggtgccgta cgtgcgctcc ccgagaattt tggcgctgtc
     2881 gcccgtcgcc cgggggtggt cagccccttc cagcaccgcg gcgccaatac cgcgaatgga
     2941 gacgttggag atgggcgcga tgtcggtgat acccagaccg cagtttgcgc tgcggataaa
     3001 attgtcgcgg cagcggtccg agagcttgat gcggcagttc tccagttcga gcgtggtgcc
     3061 gctctgaacg aggatcgccg aatcaagcag ccagaggtct cgaggcccgt ctggggcgta
     3121 attggttctc gggatgacga cgcggcggcc cgaagcggcc gcggccgcga cggcctggtt
     3181 gatgcgctcg gagtccgacc cctggtacgc gttcggagtg acgggttcgc caggcgcggg
     3241 gatggtttcc tgtgcaacgg ccgggctaac ggacaaaacg cacagaatgg ctgcggcaag
     3301 gatggtaaag ggcgttctac ggcgtgctga tggcatcaca caatccccct tgtgcgtaat
     3361 taagctcttt aatcgggcgt acctcgcata tcctcgagta tacggacgtg ctttgcgcag
     3421 catcaacgtg cgctaggcat cgcatgctgt tcgggtggct gcagcgtaat gcgcgcacgt
     3481 aacggggcca tcaccggccc ggtgcagccg aagttgaccg tgtattcgcc ttcaggcagc
     3541 tcgaaggtct cgatcgctgc gccggagagc gccggctcat ccaatggaag accatactgc
     3601 cggagaggtt cgcccggcca gagaatggca tattgcccgt cgtgaagggt cgcggggcat
     3661 tcgatccggc gcccaccgat ctcgacccag ggatccgtca gcgtgcggac gtcgagtgcc
     3721 ggaagggccc ggatctcgcc gatggcacag gataccgctg ttcgagcggg caggccattg
     3781 taatagaagt tgagacggcg cacctgtgag tagtcaatgg gatccgtttc cggccgccgc
     3841 agctgatgat accgccaccc cgtgtaattg ttggcgatgt agaaatcggc ggctctcgcg
     3901 ccatcatgca actggagctt gaagctgccg ccgttgccgt cgcctcgcag ccacaagcct
     3961 atcgcacggt gatccgagag gtcgagtggc tgcgcaaacg gtttgccgat gaccgtccag
     4021 cctgtgtcgg tgctcaattc gctctccgcc gaatacaccg cgcaggaacc gccttcgccg
     4081 ggaccgtcgg tggacagggc gagttgctgc gttacgccct cgagcgtcga acccgcttct
     4141 ccgtgtgcga tggcagccac gcccggggcc gcgccgggca ggtagggcgc aaggtcgtca
     4201 aaggtttcca gggtcaccgc gtccgcagcg tcccaggacg gtccgggagc agtccacggc
     4261 ccgggctgtg catggatttg aaggccaacg cgcgcaggac cc
//
LOCUS       DAOPBQ010000312         4302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16848_length_4302_cov_3.166000, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000312 DAOPBQ010000000
VERSION     DAOPBQ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16848_length_4302_cov_3.166000"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            485..1759
                     /locus_tag="PKW60_10230"
     CDS             485..1759
                     /locus_tag="PKW60_10230"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPJ99851.1"
                     /translation="MNSVKLILDGQEYESRVVVGSEGEKAFDITSLRTATGYITLDPG
                     YANTGSCESAITFIDGEKGILRYRGYPIEELAVSARFPEVCHLLVYGHLPNREELLEW
                     RRQLTLNSYAHESMINVFDHYPPHAHPMAILSAMTSSLSAFYPEANTPETLDLAIIYL
                     LAKAKTLAAYSYKKSRGEAIMYPLAELSYAANFLRMMFGHPAEPYEVSPVIERALDLL
                     LIIHADHEQNCSTSTVRMVGSSQANLFAAVSAGVCALWGPLHGGANQAVIEMLENIKA
                     DGGNYKKYIKMAKDRQSGFRLMGFGHRVYKSFDPRARILKKACDDVLNEMGTSDPMLD
                     IAKHLEEAALKDDFFIERKLYPNVDFYSGIIYRAIGIPTNMFTVMFALGRLPGWIAHY
                     REMRLERGERIHRPRQIYTGENVRKWVPLDER"
     gene            complement(1842..2027)
                     /locus_tag="PKW60_10235"
     CDS             complement(1842..2027)
                     /locus_tag="PKW60_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99852.1"
                     /translation="MKKMLALVCRFCPVCILRRRFPESGYGRFTRRIERACPFCRAYD
                     ELHGVGTAQAPHNAGNE"
     gene            2295..2879
                     /locus_tag="PKW60_10240"
     CDS             2295..2879
                     /locus_tag="PKW60_10240"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPJ99853.1"
                     /translation="MSGLKIPLSALERGEHEIDITVPEGGLRPPEAKALEVGPVHIQG
                     RISRVEPRFVFIGTLTGEYLRQCDRCLAPVQVPFELEVIWPFEPGPAGEAAELAAESG
                     DGDLEDNESDTVYFDGGEIDLAAPAWEELVLSAPLKTLCEDDCAGLCPHCGANRNTAP
                     CHCGGKAPLTEFGNSGFSGLAALFPELDPKRSKE"
     gene            2884..3066
                     /gene="rpmF"
                     /locus_tag="PKW60_10245"
     CDS             2884..3066
                     /gene="rpmF"
                     /locus_tag="PKW60_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPJ99854.1"
                     /translation="MPVPKRRTGKTRRNTRRSHHALVKPCLSECPSCGERIQPHRVCP
                     KCGHYKERLSIKIGTE"
     gene            3202..4233
                     /gene="plsX"
                     /locus_tag="PKW60_10250"
     CDS             3202..4233
                     /gene="plsX"
                     /locus_tag="PKW60_10250"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPJ99855.1"
                     /translation="MRIALDAMGSDNAPGPEVRGAVEASLQDGTEYVLVGDQERLEQA
                     LARYKRRGRVSIVHASETIRMDDQPVQAVRQKKDSSLLVALRLVKSGEADACVSAGNT
                     GAVMVAARTTLGPIRGVSRSAICQTVPTVKKPVLLIDMGANVDCTARQLCDFAEMGML
                     YMEHVLRRKNPRVGLLNIGEEHVKGNDLAKGVHRNLSASGHINFVGNIEPRAIYHGDA
                     DVVVCDGFVGNVVLKTTEATAALIKQRLHRELMSTWLSRLGAFFSRGAYRRLKQEIDP
                     NMYSGAPLLGVKGVVIILHGSSSSLGVKNALLGARRAVHSRINEHIRYGIERLRSTEA
                     HLNAQEANP"
BASE COUNT          885 a         1241 c         1356 g          820 t
ORIGIN      
        1 ggatctgcag ggccacacaa cgtttgacca gtacaagggc gaagagatcc cgtgggtggc
       61 gctcattggc cgcgacgggc tggttcatta ccttggcgca tacagcaaag ctgaattcga
      121 cggaaaactc gtcacgttgc tggacgccga gcgttgattc tccggcgggc cggagcgcat
      181 ctgcgcccgc cggaaatgcg cggtacagcg ggtgcaagtg tgggcagcag cgcgaaaatg
      241 gcgcgccgta gaggccgcag gaccggcgca taggcgctat cgcattgtgt gtcaaggagt
      301 tgacattata cagcgatttc gatacagttt tttgcttggg cgaggcctgt agtttttcgt
      361 atgggtcgtt cggcagcgaa acaccgtttt ggcgcaccta tacgcatacg tgggcagaga
      421 gtcagacggg tgggaacgtt tgcattcatc cgcgcgggcg aatcaggagg gagatacggg
      481 cagtatgaac tcagtcaaac tgattctgga cggacaggaa tatgagtctc gggtggtcgt
      541 tggctccgag ggggaaaagg cgtttgatat cacctctctg cgcacggcga cgggctacat
      601 cacgcttgat ccgggctatg ccaacaccgg ctcgtgcgag agtgcgatca cttttattga
      661 cggcgagaaa ggtatcctga ggtaccgggg atatcctatc gaagaactgg ccgtctcggc
      721 gcgctttccc gaggtatgcc atttgctggt ctatgggcac ctgcccaatc gcgaagagct
      781 cctggaatgg cgccgccagc tcacgttgaa cagctacgcg cacgaaagca tgatcaacgt
      841 gttcgaccac tatccgcctc acgcgcaccc catggccatt ctctcggcga tgacctcgag
      901 cctttccgcg ttctatccgg aggccaatac gccggagacg ctcgatctgg cgatcatcta
      961 cctcctggcc aaagccaaga ctctggcggc ctactcgtac aaaaaatcac gcggcgaggc
     1021 cattatgtac ccgttggccg agttgagcta tgccgccaac tttctccgga tgatgtttgg
     1081 ccaccccgcg gaaccgtatg aggtctcgcc cgtgatcgag cgcgcattgg acctgctgct
     1141 catcattcac gccgatcacg aacagaactg cagcacctcc accgtacgta tggttggaag
     1201 cagccaggcc aacctgttcg ccgcagtgtc cgcgggcgtg tgcgccctct ggggaccgct
     1261 ccacggcggc gccaaccagg ccgtcattga aatgctcgaa aacatcaaag ccgatggcgg
     1321 caattacaaa aagtatatca agatggccaa agacaggcag tcgggattcc ggctgatggg
     1381 cttcgggcac cgcgtctaca agagcttcga tccccgcgcg cgcatcctga aaaaggcatg
     1441 tgacgacgtc ctcaacgaaa tgggtacgtc cgatccgatg ctggatatcg ccaaacacct
     1501 tgaggaagcg gcgttgaagg acgatttctt catcgagcgg aagctgtatc cgaacgtcga
     1561 tttttacagc gggatcattt accgtgccat cggcatcccc accaacatgt tcacggtcat
     1621 gttcgcgttg ggccgcctgc ccgggtggat cgctcattac cgcgaaatgc gcctggaacg
     1681 cggggagcgc atccaccggc cacgccagat ctacaccggc gaaaacgtgc gaaagtgggt
     1741 gcccctcgac gaacggtagg ctgaacgcac cgctggcgtc cgcccagaag ccggggcgag
     1801 gggcaagacc cgcccctgag gaggcgctga aagacttcgt tttattcgtt cccggcgtta
     1861 tggggcgctt gagccgtgcc caccccgtgc agctcgtcgt aggcgcggca aaacggacag
     1921 gcgcgctcga tacggcgcgt gaaccggccg taaccggact cgggaaacct gcgccgaaga
     1981 atgcaaacgg gacaaaaccg gcataccagg gccagcattt tcttcataac taaggctcct
     2041 gaaggtgctt aggcgttttt aacccgctca ttgagacttc tggccggtcg ctagtgtgcg
     2101 cgagcgggaa cacatatgac tctgcaggga ttaagataca aggtgtgtta aatgtgagca
     2161 agggcgccgt gcaggcccgc atgtctcccg ctggaggccg ggaaaggcgg gtttgccggg
     2221 cacgccgcgc cgtatttgac gcgttatgcc ttaatttgat acgctgacgg ccgtttttac
     2281 aggaggcaga cgccgtgtcg ggcttgaaaa taccgctgtc ggctctggaa cgcggcgaac
     2341 atgagatcga cattacggtc cctgaagggg ggcttcgtcc tccggaggcg aaagcgctgg
     2401 aggtgggccc ggtccacatc caggggagga taagtcgtgt cgagccccgt ttcgtgttca
     2461 tcggaacgct gacgggggag tacctcaggc aatgcgaccg gtgccttgcg ccggtgcagg
     2521 ttccgtttga gctcgaggtt atctggccgt ttgaaccggg gccggccggc gaagccgccg
     2581 aacttgcggc ggaaagcggt gacggcgacc tggaggacaa cgagagcgat acggtgtatt
     2641 ttgacggcgg cgagattgac ctggccgctc cagcgtggga agagctggtg ctgtcggcgc
     2701 cgttgaagac attgtgcgag gatgattgcg cggggctgtg cccgcactgc ggcgcgaatc
     2761 gcaatacggc gccctgccat tgcgggggaa aggcccccct gacggaattt ggaaatagcg
     2821 ggttttcggg attggcggcc ttgtttcccg agctggaccc caaacgttcg aaggagtgaa
     2881 ctcgtgccag taccaaagag acgcactgga aagacgcgcc ggaacacgcg gcggtctcat
     2941 catgccctgg tgaagccctg cctcagcgaa tgcccgagtt gcggcgaacg cattcagccg
     3001 catcgggtat gtccgaaatg tggccattat aaagagcgtt tgtcgattaa gatcggaacc
     3061 gagtaatctg cgtgatgccc gccaacggac gacgcccggc ctattgaccg ggcgtcgatt
     3121 tgtaacggcg cgaagcttct tcacggcgca taataagcct cgggatggct tgtgctgatg
     3181 gggaaccgac aggggtagcg catgcgaatt gctctcgatg ccatggggtc cgacaacgct
     3241 ccgggtcctg aagtgcgtgg agccgtagag gccagccttc aggacggcac ggaatatgtg
     3301 cttgttgggg accaggagcg gctcgaacag gcgctggcgc gttataaacg gcgcggccgc
     3361 gtcagcatcg tgcatgcgtc ggaaaccatc cggatggacg accagcccgt gcaggcggtt
     3421 cgtcagaaaa aagactcttc tctgctcgtg gcactccgcc tggtgaaatc cggcgaggcc
     3481 gatgcctgtg tcagtgcggg aaacaccggc gcagtcatgg tcgccgcacg cacgacgctc
     3541 ggcccgatcc ggggcgtttc ccgctcggcc atctgccaga cggtgcccac cgtcaagaaa
     3601 ccggtgctgt tgattgacat gggcgccaac gtcgattgta cggcccgcca gctctgcgat
     3661 ttcgccgaaa tgggcatgct ctatatggaa cacgtgctgc ggcggaaaaa cccgcgggtg
     3721 ggcctgctca atatcggcga ggagcatgtc aaaggcaacg acctggccaa aggcgtccac
     3781 cgcaatctga gcgcttcggg ccacatcaat ttcgtcggca acatcgagcc ccgggccatc
     3841 taccacggcg acgccgatgt ggtggtctgc gacgggttcg tgggcaacgt ggtgctcaag
     3901 accacagaag ccacggcggc cttgatcaaa caacggcttc accgcgaact catgtcgacc
     3961 tggctcagca gactgggggc atttttcagc aggggcgcct accgccggct caaacaggaa
     4021 atcgatccca acatgtattc aggcgccccc ctccttggcg tgaagggcgt tgtgatcatt
     4081 ctgcacggct cgtcttcttc gcttggcgtc aaaaatgccc tgttgggtgc gcgccgggca
     4141 gtgcattcgc gcatcaacga gcacatccgc tacggcatcg agcgcttgcg cagcaccgaa
     4201 gcccatctga acgcccagga ggccaatcca tgaccgcatt tcttttcccc ggccagggga
     4261 gccaggcgcc cggcatgggc gccgatttcc gggattccag cg
//
LOCUS       DAOPBQ010000313         4286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16934_length_4286_cov_3.940440, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000313 DAOPBQ010000000
VERSION     DAOPBQ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16934_length_4286_cov_3.940440"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1914
                     /locus_tag="PKW60_10255"
     CDS             <1..1914
                     /locus_tag="PKW60_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPJ99856.1"
                     /translation="ILADFAYEWEYWLGPDGSPVYVSPSCERITGYCRQAFYDRPSLL
                     EEIVHPDDRKRARGFVFGRPDAHEAALLEFRIIDVNGQEHWLAHASQPVHDNDGHWAG
                     RRASNRVITDQKRAEELVQELSQWRDALLESANILVCVQDLDGRFLIWNNGAERITEY
                     PRDAVLGSSSLLEVLFAEPADWERSRALLLKAAREESAAHQQFEIRTKSGRPRTLACF
                     SKPILDVEGAPAGALILGVDITRQKRVEIDRRALEAQIQQTQKRESLAVMAGGIAHDF
                     NNLLMGILGNAGIVLEELPEDTRIRKNVTQIEKTARRAADLIRQMLAYSGKGRFVIET
                     LNLNIVLQDMLPLLTSALSKKAELVFDLAPNLPYMDGDTSQLRQLIMNLAMNASEALE
                     DRPGVITLRTGMMYCSKSYLGSTYLDEEHTEGDFVYFEITDTGCGMSEETQATLFDPF
                     FSTKFTGRGLGLAAALGIIHGHRGAVKVDSKPGQGTTVRALFPCGTAVRPGAQRSGAA
                     AAQDAPQSTVLVIDDEEAARLVAQEMLERSGYRVLAAADGCEGLQQYQKTPRAIDVVL
                     LDLTMPHLDGEETFHRIRAIRPDARVVLTSGYDEHEVARRFHGLGLAGFLQKPYTPTE
                     LRSKIAEALSEKA"
     gene            complement(1929..2585)
                     /gene="thiE"
                     /locus_tag="PKW60_10260"
     CDS             complement(1929..2585)
                     /gene="thiE"
                     /locus_tag="PKW60_10260"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPJ99857.1"
                     /translation="MRLAERMRRFDATDLYVVITKEFCNGRPALTVLDAVLEAGVQLV
                     QLREKDSTDRDLYARALAFRERTRAHNALLIIDDRVDMALAVDADGVHLGEHDLPLEA
                     ARRIAPDLILGASSHNLQEALAAQDVGASYVNIGPVFPTQTKRVASGALGPEAIAEIA
                     PRLSIPFTTMGGIKTHNIAEVVRRGARHVAVVTAVTAAPDVCAAARELRALIRDPAIP
                     "
     gene            complement(2585..2785)
                     /gene="thiS"
                     /locus_tag="PKW60_10265"
     CDS             complement(2585..2785)
                     /gene="thiS"
                     /locus_tag="PKW60_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011647240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPJ99858.1"
                     /translation="MKITLNGQDHEVVPGTTIHRLILDAGHKPAATLVERNGDIVERD
                     DYASLRLDEGDAIELVQFVGGG"
     gene            complement(2840..3973)
                     /gene="thiH"
                     /locus_tag="PKW60_10270"
     CDS             complement(2840..3973)
                     /gene="thiH"
                     /locus_tag="PKW60_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HPJ99859.1"
                     /translation="MDFFDELGAWPRTRIEALIEQARPQDVTAAIAREQRTPEDLCAL
                     LSPHAQESLESMAVEARRLTRWHFGRTIGMYAPIYISNVCGADCVYCGYAVRSGNKQR
                     RLNLNAGEIRTECAALAAEGYQNVLLLTGEARRAAPVSYIADAVGIAREFFPSVSVEV
                     YSLDEEEYRLLCERGLEGVTLYMETYDQAVYRAVHLKGQKTDYRYRLDAIERAGRAGA
                     RRLSIGALLGLTDWRIDGFWTALHGRYLQRTCWQSAVSISFPRLLHTPARFSIPHLVT
                     DAELVQLMLAIRLFLPEAGFNLSTRERAEFRDHLIPLGVTMMSAGSSTRPGGYATHVD
                     ETLEQFAIEDQRPPAKVAEAIRAKGYETVWKDFDYAFDDTANQ"
     gene            complement(3997..>4286)
                     /locus_tag="PKW60_10275"
     CDS             complement(3997..>4286)
                     /locus_tag="PKW60_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99860.1"
                     /translation="CHPRVIPFHAPLNDAIYWKNHNGRDNEILYNGLLARYGTLDAAG
                     AIELARAAGVQSTVLSIVYRNSGLDFWVAYANGLDPAQNQNYVHIELNPEP"
BASE COUNT          804 a         1279 c         1392 g          811 t
ORIGIN      
        1 atccttgcgg attttgccta cgaatgggag tactggctcg ggccggacgg ttcaccggtg
       61 tatgtgtcgc cgtcctgtga gcgtatcacc ggctattgca ggcaggcgtt ttatgaccgc
      121 ccgtcgctcc tcgaagaaat cgtgcatccg gacgatcgca agcgggcgcg gggctttgtc
      181 ttcggccggc cggacgcgca cgaggccgcc ttgctcgagt tccggatcat cgatgtgaat
      241 ggccaggaac actggctggc tcatgccagc cagccggttc acgataacga cggccattgg
      301 gccggccgga gggcgtccaa tcgcgtgatc acggaccaga aacgggcgga ggaattggtg
      361 caggaactct cccagtggcg cgacgcgctc cttgaaagcg ccaatatcct cgtgtgcgtg
      421 caggatctcg acggccggtt tctgatatgg aataacggcg cggaacggat cacggaatac
      481 ccgcgggacg ccgtgcttgg gagcagcagc ctcctggagg tgctgtttgc cgagcccgcg
      541 gactgggagc gcagccgggc gcttctactt aaggccgcac gagaggagag tgcggcgcac
      601 cagcagttcg agatccgcac caaatcgggc cgccctcgca ccctggcgtg tttcagcaaa
      661 cccatcctgg acgtggaagg cgcaccagcc ggcgcgctca tcctcggtgt cgatataacc
      721 cggcagaaga gggtggaaat cgatcggcgg gcactggagg cgcagattca gcaaacccag
      781 aaacgggaga gtctggccgt catggcgggg gggatcgccc acgatttcaa caacctgttg
      841 atgggcatcc tgggcaacgc cggcatcgta ttggaagaac tgccggaaga cacgcgcatc
      901 cggaagaatg tcacccagat cgaaaagaca gcccgccgcg ccgcagatct catccggcag
      961 atgctggcct attctggaaa ggggcgcttt gtgattgaaa ccctgaatct caatatcgtg
     1021 ctccaggaca tgctgcccct gctcacgtcc gccttgtcaa agaaggccga gctcgttttc
     1081 gaccttgccc cgaatcttcc gtacatggat ggcgacactt cccaattgcg ccagcttatt
     1141 atgaatctgg ctatgaacgc ctcggaggcc ctcgaagacc gccccggcgt cattaccctg
     1201 cgtaccggca tgatgtattg cagcaagagt tacctgggtt ccacctatct ggatgaagaa
     1261 cacacggagg gtgactttgt ctatttcgag attaccgata cgggatgcgg tatgtccgag
     1321 gagacccagg cgacactgtt cgaccccttc ttttcgacca aattcaccgg gcgcggcctg
     1381 ggccttgccg cagccctggg gattattcac gggcaccggg gcgcggttaa ggtagactcg
     1441 aaaccaggcc agggaacgac cgtgcgggcc ctgttcccct gcggcacggc cgtacgtccg
     1501 ggcgcgcagc gcagtggtgc ggctgccgca caggatgcgc cgcaatcgac ggttctggtc
     1561 atcgacgacg aagaggccgc acgcctggtt gcgcaggaaa tgcttgagcg cagcggttac
     1621 cgggtgcttg ccgccgcgga cggttgcgag gggcttcagc agtatcaaaa gactccgcgt
     1681 gccatcgatg tggtgctgct ggatcttacg atgcctcatc tggacggcga ggagaccttc
     1741 caccggattc gtgcgatccg gccagacgcc cgggtggtcc tgacgagcgg ctatgacgaa
     1801 cacgaagtgg cccggcgctt tcatgggctg ggcctggccg gttttcttca gaaaccgtat
     1861 acgccgacgg aattacgctc caagatcgcg gaagcactga gcgaaaaagc gtagttgctc
     1921 tctgcgggtc agggtattgc cgggtcgcgg atcagggcgc gcaactcgcg cgcggcagcg
     1981 cacacatctg gagcggcagt caccgccgtg accacggcga catgccgggc gccgcgccga
     2041 accacttcgg caatgttgtg tgttttgatt ccgcccatgg tggtgaacgg gatgctcagc
     2101 ctgggggcga tctcggcgat tgcctcgggg ccaagcgccc cggaagccac gcgtttggtc
     2161 tgcgtcggga aaaccgggcc gatattcacg taactcgcgc cgacgtcctg cgcggccagc
     2221 gcttcctgca gattgtggga cgaggcgccc aggatcaggt cgggtgcgat acggcgcgcg
     2281 gcctccaggg gcaggtcgtg ttcgcccaga tggacgccgt cggcatcgac cgccagggcc
     2341 atatccacgc gatcatcaat gataaggagc gcgttgtgcg cccgggtacg ttctcgaaag
     2401 gcaagggcgc gcgcatacag gtcgcgatcg gtgctgtctt tttcgcgcag ctgtacgagc
     2461 tggactcccg cttccagaac cgcatcgagc acggtcagcg caggccgccc gttacagaat
     2521 tccttggtga tcaccacata gaggtcggtg gcgtcaaagc gtcgcattcg ttcggcgagg
     2581 cgcatcatcc gcctccaacg aactgcacga gctcgatggc atcgccttca tccagccgca
     2641 gtgaagcgta gtcgtcgcgt tcaacgatat cgccgttacg ttcaacgagc gtggcggccg
     2701 gtttgtgccc ggcgtcgaga atcagacggt ggatggtcgt tcccggcacg acttcgtggt
     2761 cctggccgtt cagtgtaatt ttcatggcac aaagctccag gtgttccagg gcagaacccg
     2821 cgcggatggg cgggcggggc tattggtttg cggtgtcatc gaaggcataa tcgaaatctt
     2881 tccagaccgt ctcgtatcct ttggcgcgaa tggcttccgc gactttcgcg ggaggccgct
     2941 ggtcttcgat ggcgaattgt tcgagcgttt catcgacgtg ggtggcatag ccgccggggc
     3001 gggtcgacga accggcgctc atcatcgtca cgccgagggg gatcaggtgg tcgcggaatt
     3061 cggcccgttc gcgcgtcgag aggttgaaac cggcctcggg caggaacagg cggatggcca
     3121 gcatgagctg gaccagttcc gcatccgtga caaggtgcgg aatggaaaac cgtgctggtg
     3181 tgtggagcag ccgcgggaac gagatggaca cggcactctg ccagcaggtc cgctgaaggt
     3241 accggccatg cagtgcggtc cagaatccgt cgatccgcca gtcggtcaga ccgagcagcg
     3301 cgccgatgct gagccggcgc gctcccgccc gcccggcccg ttcgatcgcg tcaaggcggt
     3361 agcggtaatc ggttttctgg cctttcagat ggacggcccg gtagaccgcc tggtcgtagg
     3421 tctccatata gagggtcacg ccctcgaggc cccgctcgca cagcagccgg tactcctcct
     3481 catcgagaga atagacttcg acactcacgg acggaaagaa ttcgcgcgcg atcccgaccg
     3541 cgtcggcaat gtaggaaact ggtgcggcgc ggcgggcttc acccgtcagc agcagcacgt
     3601 tctggtagcc ttcggcggcg agcgccgcgc attcggtccg gatctcgccg gcgttgagat
     3661 tgagccgccg ttgtttgttg cctgaccgga cggcatagcc gcaatagacg caatcggcgc
     3721 cacacacatt ggagatatag atgggggcgt acatgccgat tgtccgcccg aaatgccatc
     3781 gggtcaggcg gcgggcctcc acggccatcg actcaaggct ttcctgagca tggggcgaga
     3841 gcagcgcgca caggtcttcg ggtgtacgct gttcccgcgc gatggcggcg gtgacatcct
     3901 gcgggcgggc ctgttcgatc agggcttcaa tgcgggtgcg tggccacgcg cccagctcgt
     3961 caaaaaaatc cactggcagc ctccgtgagt cctggctcac ggctccggat tgagttcaat
     4021 gtgaacataa ttctgattct gagccggatc cagtccgttg gcataggcta cccagaaatc
     4081 cagacccgag ttgcggtaca cgatgctgag caccgtgctc tgcacgcccg ccgctctcgc
     4141 cagttcgatg gcacccgcgg catccagcgt tccatagcgt gccagtaagc cgttatacag
     4201 gatctcgttg tcgcgtccgt tgtgattctt ccaataaatg gcatcattca acggggcgtg
     4261 gaacggaatg actcggggat ggcagg
//
LOCUS       DAOPBQ010000314         4270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_16990_length_4270_cov_3.887070, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000314 DAOPBQ010000000
VERSION     DAOPBQ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16990_length_4270_cov_3.887070"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PKW60_10280"
     CDS             <1..585
                     /locus_tag="PKW60_10280"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPJ99861.1"
                     /translation="PVKNKAIILSDGAWLAPASTELGAWKPFADRSDDKGLTWSRSAD
                     FAIDPAEVVGEGAIQPTFWESTPGRVHALLRTTAAKIGRADSMDGGRTWSPMRLTAMP
                     NNNSGIDVDRLDANRLLMLYNPIGKDWGPRTPLNLAVSSDNGETWQDLVSLEIEEGEY
                     SYPSIIRSNDGIALCYTWRRERVRCWQIPASVFE"
     gene            complement(738..1112)
                     /gene="rpsI"
                     /locus_tag="PKW60_10285"
     CDS             complement(738..1112)
                     /gene="rpsI"
                     /locus_tag="PKW60_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPJ99862.1"
                     /translation="MAIGRRKNASARVRLKPGTGVVTVNGRPLDAYLAREVLVMLAKQ
                     PFDVTDTVNRFDIRARCSGGGLTGQAGALRLGIARALVELDEKYRPVLRKYGLLTRDA
                     REVERKKYGRPGARKRFQFSKR"
     gene            complement(1180..1608)
                     /gene="rplM"
                     /locus_tag="PKW60_10290"
     CDS             complement(1180..1608)
                     /gene="rplM"
                     /locus_tag="PKW60_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPJ99863.1"
                     /translation="MKTYVPREGDIKKKWLVIDADGKVLGRVAAEAARLLRGKHKPQF
                     TPYLDCGDHVVIINADKACVTGNKAQDKKYYRHSGYPGGLKETTYEKLMAKHPTRAMM
                     FAVKGMLPHNRLGRQLATRIRIYAGGEHPHEAQNPEMHTF"
     gene            2008..2772
                     /locus_tag="PKW60_10295"
     CDS             2008..2772
                     /locus_tag="PKW60_10295"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPJ99864.1"
                     /translation="MIIDCHGHCSIDGSYSLSADDTVRSLDLYGVDQICCSAPVTSGD
                     AEPGLIRQRNDLILGAMRKYPDRIMGMCFVNPGHAREAQDEITRCVVDNGMVGVKLYN
                     QYRICDTVQFPIIERCIDLGVPILMHAGYPAAPELRALQPRISTGMHFAGAARRYPEA
                     LLICAHIGGGGDWERQLKGLREAPSVYVDTGGSVIDAGMIERCVRELGIERILFACDG
                     SLERGLGKIRDANLTDSERASLYSANFLKILARRKI"
     gene            2769..3533
                     /locus_tag="PKW60_10300"
     CDS             2769..3533
                     /locus_tag="PKW60_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99865.1"
                     /translation="MIIDANTWIGHWPFRSLPKRSARDLLEQMDRLKISKALVGNLNG
                     LLYKDVHESNHELAAEIQRHADRLVPCALINPLYDGWRDDLRQCREKFGMPVLRLTPD
                     YHGYALSDPCASELISEAHGLGMRVAFYWRIVDPRGRHWLDPGREADGALVEQVMAQF
                     PEHPFLMLNFTRVLSRPDTQAPCLYDITYFLGRNGLLLMDHLAAHGAARFVLGTTMLM
                     RYGSAPLLALEKCELSSKEREAILWRNIAKLIPELS"
     gene            3580..>4270
                     /locus_tag="PKW60_10305"
     CDS             3580..>4270
                     /locus_tag="PKW60_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99866.1"
                     /translation="MTHRERVRAILNYAPYDRMPLVHFGFWAETLEKWAAEGHITKEE
                     AESWGDGNPADSAISAKLGFDFNYYCCFAPHTGLYPEFEREVIEELPDGGKKVRNAWG
                     VIELEKPGATSIPAEIDHLLKTRRDWEEHYKHRLQFNPERITQYRVLVNGNMVPWTEG
                     GLEFLQKDDRDYHFGLHCGSLFGEIRNILGVAGSSYLFAEDDALFTEIIDTRAELSYQ
                     TVKTVLETGAKF"
BASE COUNT          840 a         1364 c         1216 g          850 t
ORIGIN      
        1 cccgtcaaga acaaggccat catcctttcg gacggagcgt ggctcgcacc tgcctcgacc
       61 gaactcggcg cctggaagcc tttcgccgac cgttccgacg acaaagggct gacctggagc
      121 cgaagcgccg attttgccat tgacccggct gaagtcgtcg gcgaaggggc cattcaacca
      181 acgttctggg aatcgacgcc gggccgcgtg cacgctctct tacgcaccac ggcggcaaag
      241 atcggccgcg ccgattcgat ggacggcggc cggacctggt cgcccatgcg tctgactgcg
      301 atgcctaata acaacagcgg gatcgatgtc gacaggctcg atgcgaatcg tcttctgatg
      361 ctctacaatc ccatcggcaa ggactggggc cctcgaacgc cattgaacct cgccgtttcg
      421 agcgataacg gcgaaacgtg gcaggatctc gtcagcctgg agatcgagga gggtgaatat
      481 tcgtacccgt cgatcatccg gagcaatgac ggcatcgccc tgtgctacac ctggcgccgc
      541 gaacgcgtgc gctgctggca gatacccgcc tctgtgttcg aataaatcgg cgccgcgaaa
      601 acaaagtacg gccgtggggt ccacccggga ctccacggcc gtttgtgtag gtgcgggctg
      661 acgtagcgaa aaagcagcct cgcctccgaa agggcttcgg agacgaggct gagtaatggt
      721 tcgctggctc gagcggttta gcgcttcgag aactggaaac gtttgcgggc gccggggcgt
      781 ccgtatttct tgcgttcgac ttcgcgtgcg tcgcgggtca agaggccgta ttttcgcaga
      841 accgggcggt atttctcgtc cagttccacg agcgcacggg caattcccag acgcaaggcg
      901 ccggcctggc cggtcagccc gccgccgctg caccgcgcgc ggatatcaaa gcgatttacg
      961 gtgtccgtca cgtcgaacgg ctgttttgca agcatcacga gcacttcacg cgccagatag
     1021 gcatccaggg gacggccatt gaccgtaacc acacccgtgc cgggtttcag gcgcacacgc
     1081 gccgaggcgt ttttccgccg gccgatggcc atcacttcat tcgtctttgt gtcgactgcc
     1141 actgggggtt cctctcctca atcctcgcct tcgtttttgc tagaaggtgt gcatttccgg
     1201 attctgtgcc tcatggggat gctcgccgcc cgcgtagatg cgaatgcgcg tggccagctg
     1261 ccgccccaac cggttgtggg gcaacatgcc tttcaccgca aacatcatgg cacgcgtcgg
     1321 gtgcttcgcc atcagctttt cgtaggtggt ttctttcagc ccgccgggat acccggagtg
     1381 ccggtaatac ttcttgtcct gcgccttgtt tccggtgacg cacgctttgt ccgcgttaat
     1441 gatcaccaca tggtcgccgc aatccaggta cggggtaaac tgcggcttat gcttgccgcg
     1501 caggagtctg gccgcctcag cggcaacccg gccgagcacc ttgccatccg catcgatgac
     1561 cagccatttc tttttgatat ctccctcacg ggggacatac gttttcacga cttctctctc
     1621 cctaacggcc gggtcaggcc gtaaaagcac acaattcagc aacttgtgag gacaacgcga
     1681 gccgaaagtc tacctgacta cgagcccaat gtcaagaaag atgtcgcctc tgaattccgg
     1741 caattttcgg ccgggattac gcaggctttc cagcgctgcg cccggttaac ggcattctat
     1801 cctccagagg gtccagaaaa cacctccatc gccgccgcac tactcaagcc tccgccctgt
     1861 ccccggcccg tttaccccgc ccgccccgca gcttgcactg cccccgccgt cagtgtgtga
     1921 tgaagatgaa agattgcaat tgaacctcta gtgctcaatg cgtaccattc gaatccgcag
     1981 gtagtccaag actcggggaa gatgcccatg atcatcgatt gccacggcca ttgctccatt
     2041 gatggttcct attcactgtc tgcggacgat acggttcgca gtctcgatct gtacggtgtc
     2101 gaccaaatct gctgctccgc acccgttacc agcggcgatg ccgagccggg cctgatccgc
     2161 cagcgcaacg accttattct gggtgcgatg cgaaaatatc ccgatcgcat catgggcatg
     2221 tgcttcgtga atccgggcca tgcccgcgaa gcccaggacg agatcacccg ctgcgtggtg
     2281 gacaatggaa tggtcggggt gaagctgtac aaccaatacc gcatctgcga taccgtgcag
     2341 tttcccatca tagaacgctg tatcgacctc ggcgtgccga ttctcatgca tgcgggctat
     2401 cccgccgcgc ccgaattgcg ggcactgcag ccgcgcatct ccacgggcat gcatttcgcc
     2461 ggggcggcac ggcgctaccc tgaagccctg ctgatctgcg cgcatatcgg tggcggcggc
     2521 gactgggaac gccagctcaa aggcctgcgt gaggccccga gcgtgtatgt ggacaccggc
     2581 gggtcggtca tcgacgcggg catgatcgaa cgctgcgtgc gtgaactcgg cattgaacgc
     2641 atcctgttcg cgtgtgatgg cagcctggag cgcggcctcg gaaagatccg ggacgccaat
     2701 ctcaccgact ccgagcgggc cagcctctac agcgccaatt tcctgaagat cctcgcgagg
     2761 agaaagatat gattattgat gcgaacacct ggatcggcca ttggccgttc cgctcgctgc
     2821 cgaagcgttc cgcccgcgac ctgctcgaac agatggatcg gctgaagatc agcaaagccc
     2881 tcgtcggaaa tctcaacggc ctgctctata aggatgtgca cgagtccaac catgagctgg
     2941 cagccgagat ccagcgccat gccgaccgcc tcgttccctg cgcgctgatc aacccgctct
     3001 acgacggctg gcgagacgat ctgcgccagt gccgggaaaa attcgggatg cccgtcctgc
     3061 gcctcacgcc ggattaccac ggctacgcgc tgtcggaccc ctgcgcatcg gaactgattt
     3121 cggaggcaca cggcctcggc atgcgcgtgg ccttttactg gcgcatcgtg gatccccggg
     3181 ggcgccactg gctcgatccc ggtcgcgaag ccgatggggc gctcgttgag caggtgatgg
     3241 cacaattccc cgagcatccg ttcctgatgc tcaatttcac gcgcgtgctc agtcggcccg
     3301 acacccaggc gccgtgcctt tacgatatca cgtattttct ggggcggaac ggcttgctgc
     3361 tcatggatca tctggccgca cacggcgcag cacgctttgt actcggcacc accatgctca
     3421 tgcgctacgg ctccgcgccg ctactcgccc tcgaaaagtg cgagctgtcg tctaaagaac
     3481 gcgaggccat cctgtggcgc aatattgcaa agttgatccc cgaattaagt tgatgcaacc
     3541 ctgattacag gcagatgttc cactccctgg gaggaggcca tgacacatcg agaacgggtc
     3601 cgggcgatcc ttaattatgc cccgtacgac aggatgcctc ttgtccattt cggattctgg
     3661 gcagaaaccc tcgagaagtg ggccgcagaa ggccatatca cgaaagagga ggccgagtcc
     3721 tggggcgatg gcaatcccgc cgacagcgcc atcagtgcaa aactcggctt tgacttcaat
     3781 tattactgct gttttgcccc gcacaccggc ctctacccag aattcgagcg cgaggtcatc
     3841 gaggaactcc ccgatggcgg caaaaaagtg cgtaacgcct ggggggtgat cgagctagaa
     3901 aagcccggtg caacctccat ccccgccgag atcgaccacc tcctcaagac ccgccgcgac
     3961 tgggaggagc attacaaaca ccgcctgcag ttcaaccccg agcgcatcac ccagtaccgg
     4021 gtcctcgtga acggcaacat ggtcccctgg accgaaggcg ggctcgaatt cctccagaaa
     4081 gacgaccgtg attaccattt cggactccac tgcggcagcc tcttcggcga aattcgcaac
     4141 atcctcggcg ttgccggctc cagctatctt tttgccgaag atgacgccct gtttaccgag
     4201 atcatcgaca cccgggccga gctttcctat caaaccgtca aaaccgtcct cgaaaccggc
     4261 gcgaagttcg
//
LOCUS       DAOPBQ010000315         4266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17012_length_4266_cov_3.659226, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000315 DAOPBQ010000000
VERSION     DAOPBQ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17012_length_4266_cov_3.659226"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..3626)
                     /locus_tag="PKW60_10310"
     CDS             complement(9..3626)
                     /locus_tag="PKW60_10310"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPJ99867.1"
                     /translation="MRNNLRFALIIAISIGLVAAVWILLHGGEHSSGTEADGTAGIAV
                     SDSHQPGPAAVAPRIPAPERLDSDALGDRIATSKTSPGGAAAFRGALVGRIRGYDGNP
                     LAGATVLLLQIAGEDRFIRPDAPARREAQSRQDGSFEFPGLPLGTYSVLAHSGSHAQS
                     QVAYLSLEAPIADVDLKLGPSGHVSGVVRDASGQPVEGAEVWALQSPIRSRYGAGRGF
                     TPPGGPESLLQLMPGSQKTAADGVFRFTNLPAVSTRLLAMPSRAAAALSGPVMPSYDG
                     LDILLAPGGVIRGRVVLGGSDEPVAGVTVVANGSFATEHKSVVTSDEGHFEFPALRPA
                     VYEVAVVDERFALAQGAVPQVQIREGEESDPVLVQVVPAATVAGRVLKQDSGDPVAGI
                     SVAARSETLRSLVHVETAADGSYSIRGMTPGKHHVFVAEPGLRRLPAVRGAATQATIS
                     LAEGQTLTGLDFKVRFGGMVQGQVVDEAGKPAPFADIMISRADGGGIRVEKADAQGLF
                     AITGATPGARLLLRARRMHLVSQEPYPVDVPLDKSSASATLVVSQGARLQGQVLRSSG
                     APLEQAPVYLNSPRPASVQLEPTQLTGSGGTFAWDGLPGGEYQVGVAVRNRAADVEPP
                     RATVTLAPGEESAPVNLVYDGPVGGDDALEAGETGDLSIRGRVTDPRGNPIPGADVYA
                     RSGTGFGHTAQTLHDGTFEIAGIAAGDYSIGALHRDYMSADSQEVRAGEASVALVLQP
                     AGRVSGQVIDAATGQPLQEFEVAEVSARQLEREQPVAYINWQPFFDPDGQFALVMSQS
                     RVERVLMARAEGYAPEQQFIGQVEAGQHLDNLVFRLRPGAVIEGTVHDASGKAIPGAS
                     IFQGVDERRSAALARTDTSGAFRVAGLEQGSIDLTASHPSYASATVTLTARLGTVTRA
                     DFVLGAGGAVEGYVSRNGQALEGQAVSLNLVNAHQNAMTDATGYYAFRRLPEGEGTVT
                     VSLRGDASGDQRQYLNAERSVIVADGRVTRADFEFSSQDASVEGIVTVEGVPASVAYV
                     SLTVASSGGEQHTGTRIEANGFYRLENLSPGSATLDVSLRTGRDGRNKSLQFELAAGQ
                     QLRQDVDFGPVSSVMGTLRGVRSGEQAHIAVISGEVQMERPTLEDVQSLLNLMSGYGD
                     VAADGTFEVENLEPGTYTIVGVAILGEPETELEALANARYVTRVIHVEEGQPLLVNLS
                     L"
     gene            complement(3684..>4266)
                     /gene="lpxK"
                     /locus_tag="PKW60_10315"
     CDS             complement(3684..>4266)
                     /gene="lpxK"
                     /locus_tag="PKW60_10315"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPJ99868.1"
                     /translation="AIGEYDCDTLILDDGYQYVRLERDENVLVIDATNPFGNGHLLPR
                     GLLREPLSAMERATHVLLTRCDQAESLEPVLEAIERFCPDVPVRRTRHAPSMLWRVAT
                     GERLPLSLLREGRICAVCGIGNPEAFFETLEGLGAELTSRMAYPDHSAFPVETLPRDM
                     LVVTTEKDAMRLEEPGANIVALGIDLEDMDGEP"
BASE COUNT          768 a         1466 c         1262 g          770 t
ORIGIN      
        1 cggctccgct acaggctcag gttgaccaga agcggctggc cttcctcaac gtggatgacc
       61 cgggtcacgt accgcgcatt ggcaagggct tcgagctcag tctccggctc gcccaaaatt
      121 gccacgccta cgatcgtgta ggtgcccggc tcgagatttt cgacctcaaa cgttccatct
      181 gcagccacgt cgccatagcc ggacatgagg ttcaggagcg actgcacatc ctccagcgtg
      241 ggccgttcca tctggacttc acccgatatc accgcgatat gtgcctgttc accgcttcgc
      301 acgccgcgca aggtgcccat gacggacgac acgggaccga aatcgacgtc ctgccgcagc
      361 tgttgccccg cagccagctc aaactgaagc gacttgttac ggccgtcgcg ccctgtacgg
      421 agcgacacgt cgagagtcgc cgaaccgggg gagaggtttt cgagccgata aaatccgttc
      481 gcctcgatgc gggttcccgt gtgctgctcg ccgccgctgg atgccaccgt cagggataca
      541 tacgccaccg aagcgggtac cccctccaca gtgactattc cttccacgga ggcgtcctgg
      601 ctcgaaaact caaaatcggc acgcgtaacc cggccatcgg ccacgatcac cgagcgttcg
      661 gcattcaaat actggcgctg atcgccgctg gcgtctcccc gcaggctcac ggtcaccgtc
      721 ccctcaccct cgggcagccg ccggaacgcg taatagcctg ttgcatctgt catggcgttc
      781 tggtgcgcgt ttacgaggtt cagagacacc gcctgccctt ccagggcctg cccgtttctc
      841 gagacatatc cttccaccgc tcctcctgcc ccaagcacga aatccgcgcg ggtcaccgtg
      901 cccaggcggg cggtgagtgt cacggttgcg gacgcgtagg acggatgaga tgccgtcaaa
      961 tcaatgctgc cctgttcaag gccggccacc cggaaggcgc cgctcgtgtc tgtgcgggcc
     1021 agggccgcac ttcgccgctc gtccactccc tggaatatgg acgcgcccgg aatggcttta
     1081 ccggatgcat cgtgcaccgt accctcgatc accgcgccgg gccggagccg gaaaacgagg
     1141 ttgtccagat gctggccggc ttccacttgg ccgatgaact gctgttcggg cgcgtacccc
     1201 tccgcacgcg ccatcagcac gcgttccacc cggctctggc tcatgaccag cgcgaattga
     1261 ccgtccgggt caaagaacgg ctgccaattg atgtaggcga ccggctgctc gcgctccaat
     1321 tgacgcgccg acacctccgc gacctcaaat tcctgaagcg gctggccggt ggcggcgtcg
     1381 atgacctgcc cggacactct cccggccggt tgcagcacca gggcaacgct tgcctctcct
     1441 gcccgcactt cctgagagtc tgcagacatg taatcgcggt gaagcgcgcc tatggagtag
     1501 tctcctgcgg cgatcccggc aatttcaaac gtgccgtcgt gaagcgtttg ggccgtgtgc
     1561 ccgaatcctg ttcctgatcg ggcgtacacg tctgcgccag gaatcggatt gccccgtggg
     1621 tccgtcacgc gcccgcgaat cgacagatcc ccggtctccc cggcctccaa agcgtcgtct
     1681 ccgccaaccg ggccgtcgta gaccaggttc accggagcac tctcttcgcc tggcgccagc
     1741 gttaccgttg cccgcggggg ctccacgtca gctgcccggt tcctgaccgc gacacccacc
     1801 tggtactctc ctccgggaag gccatcccag gcaaacgtgc cgccgctccc ggtcagttgc
     1861 gtaggttcca attgaacact cgctgggcgg ggcgaattga gatacaccgg cgcctgttcc
     1921 agcggcgccc cggacgaacg aagcacctgc ccttgaaggc gcgcgccttg cgacaccacg
     1981 agcgttgcgc tggctgagga tttatccaga ggaacatcga ctggataagg ctcctgcgag
     2041 accagatgca tccgccgggc acgcagcaag agtcgtgctc ccggtgttgc gcctgtaatg
     2101 gcaaaaaggc cctgcgcgtc agctttttcg acccgtattc cgccgccatc cgctctgctt
     2161 atcatgatgt cggcaaaggg tgccggcttg cctgcttcat caaccacctg cccctgcacc
     2221 atcccgccga aacgaacctt gaagtccagg cctgtgagag tctggccttc ggccagcgaa
     2281 atggtcgcct gggtggcggc cccgcgtacc gcgggcaaac ggcgcaatcc cggctccgcg
     2341 acgaagacat ggtgctttcc tggagtcatc ccgcggatgc tatacgagcc atcggcggca
     2401 gtctcgacgt ggacgagcga ccggagggtt tcacttcgcg ccgccacaga gatccccgca
     2461 acggggtcgc cgctgtcctg cttcagcacg cggcccgcga cggtcgccgc gggcaccacc
     2521 tgcaccagca ccgggtcgct ttcttcacct tcgcggattt gcacctgcgg gaccgcaccc
     2581 tgggcgaggg caaaacgctc atcgacgacc gccacctcgt aaacggccgg tcgaagtgca
     2641 ggaaattcaa aatgaccctc atcggacgtc actacactct tgtgttccgt cgcgaaactg
     2701 ccgtttgcaa caacggtcac gcccgcaacc ggttcatcgc ttccgcccag cactacccgc
     2761 ccgcggatga cgccgccggg agcgaggagg atgtccaaac cgtcgtatga aggcattacc
     2821 gggccgctca gagcggcggc cgctctcgaa ggcatcgcga gcaggcgggt ggataccgca
     2881 ggcaaatttg tgaagcgaaa tacgccatcg gcggcggttt tttgcgaacc cggcatgagt
     2941 tgcagcagcg attccggccc gcctggcggc gtgaaacctc gtccagcgcc ataccggctt
     3001 cggatcggtg actgcagggc ccagacctcc gctccttcga ccggctggcc ggatgcgtca
     3061 cggactaccc ccgaaacatg gcccgagggc cccaatttga gatccacgtc cgcaatggga
     3121 gcctcgaggc ttaggtacgc cacttgagac tgcgcgtggc tgccggaatg cgctagaacg
     3181 gagtaggtcc ccaaaggcag cccggggaat tcaaagctcc cgtcttgccg gctctgcgcc
     3241 tcgcgcctgg ccggggcatc ggggcggatg aagcggtctt cgcccgcgat ctgcagcagc
     3301 aataccgtgg cgccggccaa ggggttaccg tcgtatcctc ggatgcgccc cacaagcgcg
     3361 ccccgaaagg cggccgctcc gccgggcgag gttttcgacg tggctatacg gtcgccgagg
     3421 gcatcggagt cgagccgttc cggcgcggga attcggggag cgaccgcagc cgggccgggt
     3481 tgatgtgaat ccgaaacggc aatgcccgct gtgccatcag cctcggtgcc ggaagaatgc
     3541 tctccgccat gcagcaaaat ccatacagcc gcaaccagtc caatgctgat tgctatgatc
     3601 aacgcaaacc ggaggttgtt tcgcatggat tctgctaccg atcaggccgc attcggcatc
     3661 caccgccgac cacaccttta ccgtcagggt tcgccgtcca tatcctctaa atcaatgccc
     3721 agggcaacga tattcgctcc cggctcttca agccgcatcg cgtccttctc agtcgtcacc
     3781 acgagcatat cacgaggcag ggtttctacg gggaacgcgc tgtggtcggg gtacgccatt
     3841 cgtgacgtca gctccgcgcc caggccttcg agtgtctcga aaaatgcctc cggattgccg
     3901 atcccgcaca ctgcacagat ccggccttcc ctgagcagcg agagcggcag ccgctcgccg
     3961 gtggctaccc gccacagcat ggacggcgcg tgccgcgtcc gccgcactgg cacgtccggg
     4021 cagaaccgtt caattgcttc cagcacgggc tccagcgact ccgcctggtc gcagcgggtc
     4081 agaaggacat gcgttgcgcg ctccatggcg gataggggtt cgcgcagcag acctcgaggc
     4141 aacaggtggc cgtttccaaa cgggttggtc gcgtctatca ccagcacatt ctcatctcgt
     4201 tccagccgca cgtactggta accgtcgtcc aggatcagcg tatcgcagtc gtactctcca
     4261 atggct
//
LOCUS       DAOPBQ010000316         4264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17021_length_4264_cov_4.724400, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000316 DAOPBQ010000000
VERSION     DAOPBQ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17021_length_4264_cov_4.724400"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2550
                     /locus_tag="PKW60_10320"
     CDS             <1..2550
                     /locus_tag="PKW60_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPJ99869.1"
                     /translation="ARTLRNAAIDLPGGGLKTPAGEILLRLKERRDYGRQFAEIPVVT
                     TALGSQVLLKDIAYIDDSFADTDRYSKFNGKRSVHLEVYRVGSETPIRVAKAVRQLLE
                     EYQPYLPPGIKTATNRDRSETYRQRVELLVKNGLMGLVLVLVLLGVFLEARLAFWVMM
                     GVPVSFMGSFLFLPAGDVTINMMSLFGFIIALGMVVDTNIVVGENVYYYHQQGLPFIE
                     AAIRGTREVASPVVFSVLTNIVGFVPIYFIPGYVGKTFQMIPVVVCVVFVLSLIESLY
                     VLPSQLGHQRDRVRRGISHWLHERQQGFSRRFLHWVHDYYGPALEYVLQHRYIAIAFA
                     LGALALSLSYALSGRMGFQQFPIIESDFADAQVVLPYGAPVAKTEAIMNRIQACAEQI
                     VEESGHPELVVSYTTDIGQMGSHTGRMRVELAEPAIRDSIMSTTEFTRRWREAVGEVP
                     GVEYLRFTSDSGGPGSWGRPLAVELSHRHMDLLEQTSGELADILATYPGVTDVDDGFQ
                     PGKEQLDFALKPEGKSLGLTPRDVARQVRYAFYGAEVLRQQRGRNEIKVMVRLPEEER
                     SSEHTVQSLMIQTPSGAYVPFREIAEISRGRAYTTIGRRNGRRVVEVSADITPRSRAG
                     EVLKDLQENVLPEFLREHPGLSYSFEGHQAEIRESMGSLRVTFLLALMAIYALLAIPF
                     RSYTQPLVVMLSIPFGIVGAFLGHLFMGYDLSIPSMFGIVALAGVVVNDALVMIDFAN
                     FREREEGLSPHDAIHSAAVQRFRPIMLTTLTTFGGLAPMIFETSRQAKFLIPMALSLG
                     YGILFGTAVTLVIVPSMYLAIDDVRHYLTRAGRAIVPTAKTADTQELSSAK"
     gene            2659..4035
                     /locus_tag="PKW60_10325"
     CDS             2659..4035
                     /locus_tag="PKW60_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPJ99870.1"
                     /translation="MNTIGTSTCPLRVAIIGSGPSGFYAAEALYKAGIPVEIDLFDRL
                     PVPYGLVRGGVAPDHAKIKTVTRIFDRIAGHEGFQFFGNVQVGRDIPVDELRQFYDAV
                     LFACGAETDRKLGIPGESLPGSHTATEFVGWYNGHPDYRDRVFDLSSEVAVIVGQGNV
                     SIDVGRILSKTVDELKHTDIAQHALDALAESRIRRIYLVGRRGPVQARFTAPELRELG
                     VLAACAPVVDPADLELDDASQAELEHPDNKNSQKNLSIFREFAQRPPSDAPRQCHIRF
                     RLSPVELRGTTRVESVVFERNTLEGEPFHLRARGNGETETLDCGLVFRSVGYRGIPVP
                     GVPFDEAHGVFPNSQGRIMEGSTPIPGLYAAGWIKRGPSGIIGTNKPDSAETVQSLLD
                     DLPALPPCPRRDSRELARLLNERGIRVVTYADWQAIDAKEVARGQHVGKPREKFTRLD
                     ELLGACGR"
     gene            complement(4058..>4264)
                     /locus_tag="PKW60_10330"
     CDS             complement(4058..>4264)
                     /locus_tag="PKW60_10330"
                     /inference="COORDINATES: protein motif:HMM:NF025445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 family protein"
                     /protein_id="HPJ99871.1"
                     /translation="ETAWLAYLYRRESSRWATLIYNAVHSYVTPILLLMLLWTFSPLY
                     LLGWVANIAFLRVLGFGFHTPQRA"
BASE COUNT          821 a         1250 c         1370 g          823 t
ORIGIN      
        1 gcccgaaccc tgcgaaatgc cgcgatcgac ctgcccgggg gcggtttaaa aacgccggca
       61 ggcgagatcc tgctccgtct gaaggaacgg cgggattacg ggcgccagtt cgctgagatc
      121 cccgtggtca ccacagcgct gggcagtcag gtgcttttga aagacattgc gtacattgac
      181 gacagcttcg cagacacgga ccggtactcg aagttcaacg gcaagcgctc ggtgcatctc
      241 gaagtgtacc gtgttggaag tgaaacgccg atccgggtag caaaagccgt gcgccagctg
      301 ctggaagaat accagcccta tcttcccccg ggaatcaaga cggcaaccaa ccgcgaccgt
      361 tcggagacct accggcaacg agtggagctg ctggtcaaga atgggctgat gggacttgtg
      421 ctggtgctgg tgctgctggg ggtctttctg gaagcgcggc tcgccttctg ggtcatgatg
      481 ggggtgccgg tttcgttcat gggctcattc ctgtttcttc cggcaggcga tgtcacgatc
      541 aacatgatgt ctctgttcgg gttcattatc gcgctgggaa tggtagtcga caccaacatc
      601 gtggtcgggg agaacgtgta ttactaccac cagcagggtt tgccgttcat tgaggcggcg
      661 atccgcggaa cacgggaagt ggcgtcgccg gtagttttca gtgtactgac gaatattgtg
      721 gggttcgtgc ccatttattt catcccgggc tacgtcggca agacatttca gatgattccc
      781 gtggtggtct gcgtggtttt tgtgctgtcg ctgatcgaaa gtctgtacgt cctgccctca
      841 cagctgggcc accagcgaga ccgggtgcgc agagggatca gtcattggct ccatgaaagg
      901 cagcaggggt tcagccgccg ctttttgcac tgggtgcacg attattacgg tccagcgctg
      961 gaatatgtgc tgcagcaccg gtacattgcg atcgcctttg ccctcggcgc gcttgctctg
     1021 agcctctcct acgcgttgag cgggcggatg ggttttcaac agtttcccat catcgagtcc
     1081 gatttcgccg atgcgcaagt ggtgttgccg tacggcgcgc cggtcgcaaa aaccgaagcc
     1141 atcatgaacc ggatccaggc gtgtgcggag cagattgttg aggagtcggg ccacccggaa
     1201 ctggtcgttt cgtatacgac cgacattggc cagatgggca gccacaccgg ccggatgcgc
     1261 gtcgaactgg ccgaacctgc catccgcgac tccatcatga gcacgacaga gttcacccgg
     1321 cgctggcgcg aagcagtggg cgaagtccct ggcgtagagt atctgcgttt cacgtcagac
     1381 tccggcggac cgggaagctg gggccgcccc ctggcagtgg aactgagcca ccgccatatg
     1441 gacctcctgg aacagacgag cggcgaattg gcggacatcc tcgcaaccta tcccggggtg
     1501 actgatgtgg atgacggatt ccagccgggc aaagagcagc tcgattttgc gctgaaaccg
     1561 gaaggcaaga gcctcggatt aacgccgcgc gatgttgccc ggcaggtgcg ttacgccttt
     1621 tatggggcgg aagtactgcg ccagcaacgg ggccgcaacg agatcaaggt catggtacgg
     1681 ctgcccgaag aagagcgctc gtcggagcat acggtgcaat cgctcatgat ccagaccccc
     1741 tcgggcgcgt atgtgccgtt tcgcgagatc gcggagatca gccggggccg cgcctatacc
     1801 accatcggcc gccgcaacgg gcggcgggtg gttgaagtct ctgccgacat cacgccgcgc
     1861 agccgtgccg gagaagtact caaagacctt caggagaacg tgctgcccga atttctgcgc
     1921 gagcatccgg gcctctcgta ttcgtttgaa ggccaccagg ccgagatccg cgagagcatg
     1981 ggaagcctga gagtcacgtt ccttctggcg ctgatggcga tctacgcgct gctggccatt
     2041 cccttccgaa gctacacgca gccgctggtg gtcatgctga gcatcccctt cggtatcgtg
     2101 ggcgcgtttc tgggccatct tttcatggga tacgacctgt ccatcccgag catgttcggc
     2161 atcgtggccc tggcgggggt ggttgtgaac gacgcgctgg tgatgatcga cttcgcgaac
     2221 ttccgcgagc gggaggaagg cctgtcgccg cacgacgcga ttcactccgc agccgtgcag
     2281 cgttttcgcc ccatcatgct gaccacgctg acgaccttcg gcggcctggc gccgatgatt
     2341 tttgagacat cacggcaggc gaaattcctg atcccgatgg ctttgtcttt gggatatggc
     2401 atcttgttcg gcacggcagt gaccctggtg atcgtgccgt cgatgtatct ggccattgat
     2461 gatgtgcggc attatctgac gcgcgccggc cgtgcaattg tgccgaccgc caaaacggcg
     2521 gacacgcagg agctgtcgtc cgcgaaatga gctgagcggc gctccagctt tgaaatatcc
     2581 agggcgggga gtctgtttcc ggtcctgaac cgtgccgctg aaaagataca tgagatttaa
     2641 cgcatttcgg gagcattcat gaacacgatt ggcaccagca cctgcccgct gcgcgtggcg
     2701 attatcggca gcggaccgag cggcttttat gcggcggagg cgctgtataa agccggtatt
     2761 cccgtggaga tcgacctttt tgaccgctta ccggtcccct atgggctggt tcggggcggg
     2821 gtagcgcccg atcacgccaa aatcaagacc gtgacgcgga tcttcgaccg aattgccgga
     2881 cacgaggggt ttcagttttt cggtaacgtg caggtcggcc gcgatatccc ggtggacgaa
     2941 ctgcggcagt tttacgacgc cgtgctcttt gcgtgcggcg ccgagaccga ccggaaactg
     3001 ggaattcccg gcgaatcgct gccgggcagc cacacggcaa ccgagttcgt gggctggtat
     3061 aacggccacc cggactaccg ggaccgcgtg tttgacctct cgtccgaggt ggccgtaatc
     3121 gtgggccagg gtaacgtctc aatcgatgtc ggccgcattc tgtcgaaaac ggtggacgag
     3181 ttgaaacaca ccgacatcgc acagcacgcc ctggacgcgt tggccgaaag ccgtattcgc
     3241 cgcatctatc tggttggccg ccggggcccg gtgcaggccc ggttcaccgc gccggaattg
     3301 cgcgaactgg gcgttctggc agcctgcgcg ccggtggtcg atccggccga tctggagctg
     3361 gacgacgcaa gccaggccga actcgagcac ccggacaaca aaaacagcca gaagaacctc
     3421 tccatttttc gggagtttgc ccagcggccc ccctccgatg cgccgcgaca gtgccatatc
     3481 cggtttcggc tcagccccgt ggagctgcgc ggcacgacgc gggtggaatc ggtggtgttt
     3541 gaacgaaaca cgctcgaagg tgaaccgttt catcttcggg cgcggggcaa tggcgaaacg
     3601 gaaacgctgg attgcggcct ggtgttccgc agtgtgggat atcgcggcat cccggttccc
     3661 ggcgtgccct ttgacgaggc gcacggcgtg tttccgaaca gccagggccg catcatggag
     3721 ggcagcaccc caatccccgg cctgtatgcg gcgggctgga tcaaacgcgg tccgagcggg
     3781 atcatcggaa cgaacaaacc cgacagcgcg gagaccgtcc agtcactgct ggacgatctg
     3841 ccagcgcttc ctccatgccc gcggcgggac agccgcgaac tggcccggtt gctcaatgaa
     3901 cgcggcatcc gcgtcgtgac ctacgccgac tggcaagcca ttgacgcgaa agaagtcgcg
     3961 cgggggcaac acgtgggcaa accccgggag aaattcacac ggctcgacga actcctgggg
     4021 gcctgcggcc ggtagacagc gcggactgcc aaccggatca ggctctctgc ggggtgtgaa
     4081 acccaaaccc gagcacgcgc agaaaagcga tgttggccac ccatcccagg agataaagcg
     4141 gcgagaacgt ccagagcagc atcaggagca ggatgggcgt gacgtaggaa tgcaccgcat
     4201 tgtagatcag agttgcccac cgcgacgact ccctgcggta caggtaggcc agccaggcgg
     4261 tttc
//
LOCUS       DAOPBQ010000317         4254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17069_length_4254_cov_3.590141, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000317 DAOPBQ010000000
VERSION     DAOPBQ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17069_length_4254_cov_3.590141"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PKW60_10335"
     CDS             <1..484
                     /locus_tag="PKW60_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPJ99872.1"
                     /translation="RVGAIRAGLDGAGFHEVPIMAYSAKYASSFYGPFRDAAESPPQF
                     GDRSTYQMDPANAREAMRELALDIEEGADIVMVKPALAYLDIIARAAQRFDLPLAAYH
                     VSGEFAMVKAAAANGWLDEKRAALEILTAIVRAGASLVLTYWACDAARWLQEGDPVAS
                     "
     gene            537..683
                     /locus_tag="PKW60_10340"
     CDS             537..683
                     /locus_tag="PKW60_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99873.1"
                     /translation="MTHLHVLTKIRPAKAQQELPFEKQALQWFNFSLDFAILFLRALG
                     PKVS"
     gene            717..2003
                     /gene="hemL"
                     /locus_tag="PKW60_10345"
     CDS             717..2003
                     /gene="hemL"
                     /locus_tag="PKW60_10345"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPJ99874.1"
                     /translation="MKRDTSAALWREANKVLVGGVNSPVRAFNAVGGEPFFAASGRGS
                     RIQDADGNEYIDYVLSWGPLVMGHAHPGVVEAVSTAMRLGSSFGISTEAEIRLAQRIA
                     RHFPAIEKLRFVNSGTEAVMSAIRLARGVTGRELVVKIAGCYHGHMDGLLVRAGSGLT
                     TLGQPDSQGVPNAYTQCTLVVPYNDLDAVRDAFSAYSGQIACLIVEPVAGNMGVVLPE
                     PGYLDGLREITQSAGALLIFDEVMTGFRVSMGGAQARYQVTPDLTVLGKVIGGGLPVG
                     AYGGAARIMDRLSPLGPVYQAGTLSGNPLAMAAGLATLDALEQPGVFERTEQAMTALS
                     EGLGEIARSAGIPVYQTQAGSMACLFFNERPVRNYEDATASDTKRYAAFFRGMLERGV
                     YFAPSQFEACFTSAVHSSADIDQTLAAARETFRELS"
     gene            2408..3568
                     /locus_tag="PKW60_10350"
     CDS             2408..3568
                     /locus_tag="PKW60_10350"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPJ99875.1"
                     /translation="MFRGHWRIVVCLAALLGLAGGCARTDVTGEAAKGLHLARTTFGT
                     VPDQGTAYLYTLTNANGMEVQITNYGGVVTSLSAPDATGAFADITLGYPDLEGYFDND
                     PNFGCLVGRFGNRIAKGRFTLDGEEYTLAVNNGPNHLHGGILGFGKKLWDAKAIHREG
                     AVGLQLSCVSVDGEEGYPGTLQTTVHYWLTNANELEITYEAITDKATPVNLTFHGYFN
                     LNGEGEGGILGHELMINAARYTPVDETLIPTGELAPVANTPLDFTTPMAIGARIDADD
                     EQIRRGGGYDHNYVLNSQDRSLALAARVYAPESGRVMEVYTTQPGMQLYTGNFLDGSI
                     TGKAGKAYQKRYGFCLETQHYPDSPNQPDFPSCILRPGEKYLEKTVYRFTTR"
     gene            complement(3650..>4254)
                     /locus_tag="PKW60_10355"
     CDS             complement(3650..>4254)
                     /locus_tag="PKW60_10355"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPJ99876.1"
                     /translation="VAFAPETGGAAEWHPVPLPEQTEPGPAQWLILGNGAMEVRGGSI
                     QTRQKFVDQKVHVEFRTPLLPDKDGQGRGNSGVFLQNRYEVQILDSYGFPGENNDCGG
                     IYKLAPPRVNMCAPPLQWQTFDIEFRAARFDRDWNKTEDARITVYHNGVLIHENQPIP
                     DKTTGAQVTATSEPDSLSLQDHGNPVWFRNIWLVELPPED"
BASE COUNT          793 a         1323 c         1317 g          821 t
ORIGIN      
        1 ccgcgtcggg gccattcgcg ccgggcttga tggcgccgga ttccacgagg tcccaatcat
       61 ggcctacagc gccaaatacg cctcgagttt ctatggacct ttccgggacg ccgccgaatc
      121 gccgccacaa ttcggcgatc gttccaccta tcagatggat cccgccaacg cccgcgaagc
      181 catgcgcgag ctcgcccttg acatcgaaga aggcgccgac atcgtcatgg taaagcctgc
      241 cctcgcttac ctcgacatca tcgcccgggc ggcgcagcgt tttgacctcc ctcttgccgc
      301 ataccacgtc tcgggcgaat tcgcgatggt caaggccgcg gccgccaacg gctggctcga
      361 cgaaaagcgg gcggctctgg agatcctcac cgccatcgtc cgcgccggcg cgtcgctcgt
      421 tctcacctac tgggcctgcg acgccgcccg gtggctgcag gaaggcgatc ccgtcgcatc
      481 atgacgcgat atacagctat actgaggccc gttgtagaca cggaaggaga caactgatga
      541 cgcacctgca cgttctgacg aaaatccggc ccgccaaagc tcaacaggaa ctcccctttg
      601 agaaacaggc gttgcagtgg ttcaatttta gcctggactt cgcgatcctg ttcctgcggg
      661 ccctcggccc caaggtgtcc tgaaccaggc tcacaaaacg acagggatcc atcatcatga
      721 aacgcgacac gtccgcggcc ctctggcgcg aggcaaacaa ggttctggtg ggcggcgtca
      781 acagccctgt ccgggcgttt aacgcggtgg gcggcgagcc gtttttcgcc gcctccggac
      841 gcggttcccg cattcaggac gccgatggca acgaatacat cgactatgtg ttgtcttggg
      901 gtcctctggt catggggcat gcccatccgg gcgtcgtgga ggccgtaagc acagccatgc
      961 gcctcgggtc cagtttcggc atctcaaccg aagccgagat ccgccttgca cagcgcatcg
     1021 cgcgccattt cccggccatc gagaagctcc gcttcgtcaa cagcggaacc gaagccgtca
     1081 tgagcgccat ccgcctcgcc cgcggcgtca ccggccgcga gcttgttgta aagattgccg
     1141 gctgttacca cggtcacatg gacgggctgc tcgtgcgtgc cgggagcgga ctgaccactc
     1201 tgggccagcc ggacagccag ggcgtgccga atgcctacac ccaatgcacc ctcgtggttc
     1261 cctacaacga cctggacgcg gtccgcgacg ccttttccgc ctatagcggg cagatcgcct
     1321 gcctgatcgt cgaacccgta gccggaaaca tgggcgttgt gctccccgaa cccgggtatc
     1381 tggacggatt gcgtgagatc acccaatccg ccggcgcctt gctcattttc gatgaagtga
     1441 tgacgggatt ccgcgtcagc atgggaggcg cacaggcgcg ctaccaggtt acgccggatc
     1501 tgaccgtgct cggcaaggtc attggcggcg gactccccgt gggcgcctac ggcggcgctg
     1561 cccggatcat ggaccggctt tctccgctcg gccccgtcta ccaggcgggg acgctctcgg
     1621 gcaatcccct cgccatggcc gcaggtctgg ccaccctgga tgccctcgag cagccgggtg
     1681 ttttcgaacg cacagaacag gccatgaccg cgttgtccga gggcctgggc gagatcgcgc
     1741 gcagcgcggg gatccccgtc tatcagaccc aggcgggctc catggcctgc ctcttcttca
     1801 atgaacgtcc cgtgcgcaac tacgaagacg ccaccgcctc ggataccaaa cgctatgccg
     1861 ctttcttccg gggcatgctc gagcgaggcg tctatttcgc cccgtcccag ttcgaggcct
     1921 gtttcaccag cgcggtccat tccagcgcgg acatcgatca gacgctcgca gccgcccgcg
     1981 agacgtttcg agagctgagt taaatgtatt caattgacta atgtttgtag tcgattgaat
     2041 gtatatatgt tatcgaatga ctgcaaaatt atatgcgttg actccttgaa aaatcgctcg
     2101 cgtttgcctg ccgcgccacc tgctgatcgg gccaggaagt gcctcgtcca gccccctgtt
     2161 ttcttagcaa ttctgcctgg tatggaggaa ttacctcggg ctgagccttg ccttctcctt
     2221 cccgcaggga ggggttctgc tgtttgtacg aggtgccggg ttgggcgcgg ccgtagagcc
     2281 gccgtctcaa gggccgtccc tgcagacgca tccatcctgg ttcgaggagg ccgtgagttg
     2341 aaaactggtg cggcgtgcat cattctggtg gcgggcgtca gaaaactgtg cagcggagga
     2401 cagtgcaatg ttcagaggtc attggcggat tgtggtctgt ttggcggctc ttctcgggct
     2461 cgctggtgga tgcgcgcgca cggacgtcac cggcgaagcg gcgaaaggtc tccatcttgc
     2521 acgaaccact tttggaaccg tgcccgacca gggcacggcg tacctgtata cgctgacgaa
     2581 cgccaacggg atggaggtgc agatcaccaa ctacggcggg gttgtgacat cgctatcggc
     2641 gccggacgct accggggcgt ttgcagacat caccctggga tacccggacc ttgaagggta
     2701 cttcgacaac gatccgaatt tcggatgcct ggtggggcgc ttcggcaacc gaatcgcgaa
     2761 aggacgcttc acgctggacg gggaggaata caccctggcc gtcaacaacg gtccgaacca
     2821 tctccacggc gggatcctcg ggttcggcaa gaaattatgg gacgccaaag caatccatcg
     2881 ggagggtgcg gtcggactgc agctgtcctg tgtgagtgtg gacggcgagg agggctatcc
     2941 aggcacgctt caaacgacgg tgcattactg gctgaccaac gctaacgaac tggagattac
     3001 gtatgaggcc atcacggaca aggcgacccc ggtcaacctg acgttccacg gatattttaa
     3061 tctcaacggc gaaggcgagg gcggcatcct gggccacgag ctgatgatca acgccgcgcg
     3121 gtacaccccc gtggacgaga cgctgattcc caccggggaa ttggcgcccg tggccaatac
     3181 cccgctggat tttacgacgc ccatggccat cggcgcccgg atcgatgcgg acgatgaaca
     3241 gatccggcgc ggaggaggct atgaccacaa ttacgtgctg aacagccagg atcgctcgct
     3301 ggccctcgcg gcgcgggtgt acgcgcccga atccggccgt gtcatggagg tctacacaac
     3361 acaaccgggc atgcagctct acacgggcaa cttcctggac ggatccatca ccggcaaagc
     3421 ggggaaggcc taccagaagc gctacgggtt ctgcctcgaa acgcagcatt accccgattc
     3481 gccgaatcag ccggactttc cttcatgcat cctgaggccg ggggaaaaat acctcgaaaa
     3541 gacggtctat cgctttacga cgcgctgatc acaggcaacg cggactcggc gcgtgcgtgg
     3601 cgcccacttg cagcgggaca ctcgcggcag ggcctcgcgc aggcgcgcgt caatcctccg
     3661 gcggcagttc gacgagccag atgttgcgga accagacggg gtttccgtgg tcctgcaaag
     3721 acagcgagtc cggctcggat gtcgcagtca cctgagcgcc ggtcgttttg tcggggatgg
     3781 gctggttttc gtggataagc acgccgttgt ggtagacggt gatgcgcgca tcttcggtct
     3841 tgttccagtc gcggtcaaaa cgcgcggcgc ggaattcgat gtcaaaggtc tgccactgca
     3901 gcggcggcgc gcacatgttt acgcgcgggg gagcgagttt gtagatcccg ccgcaatcgt
     3961 tattttcccc cggaaagccg tagctgtcga ggatctggac ttcgtagcgg ttttgaagaa
     4021 acacgccgct gttgccgcgt ccctggccgt ccttgtcggg aaggaggggt gtacgaaatt
     4081 caacgtgtac tttctggtcg acgaatttct gccgggtctg aatactgccg ccccggactt
     4141 ccatggcccc gttgccgagg atgagccatt gggcaggtcc cggctcagtt tgttccggca
     4201 gggggactgg gtgccattcg gcggcgccgc ctgtttcagg ggcgaaggct acgt
//
LOCUS       DAOPBQ010000318         4251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17086_length_4251_cov_3.242135, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000318 DAOPBQ010000000
VERSION     DAOPBQ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17086_length_4251_cov_3.242135"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PKW60_10360"
     CDS             <1..731
                     /locus_tag="PKW60_10360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPJ99877.1"
                     /translation="LCRDHVRWSGRHWRLLLPDSSDAFFQSMKRKHRNALQKACALMD
                     QENNDEGLSWERFIRPDQVDRFCQEADQIARKTYQRQLGASFATTESTLKKLRLLAER
                     NQWRAYILYVHGVPAAYWIGTSYGTTFHVEYTGRDPAFSQFKLGTGTMLFVKMIEDIC
                     GATDIREVDFGFGDYPYKQRFGNVNEETQTLFLFAPTFSNVSLNMLRTALAWTEKGLA
                     ALLDRLGLRARINRFRRERLGRTE"
     gene            781..936
                     /locus_tag="PKW60_10365"
     CDS             781..936
                     /locus_tag="PKW60_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99878.1"
                     /translation="MPGLAGYVAKHGAAEEHTIRGMAAPMACSRHARKMDTVCAGQAA
                     LACDANA"
     gene            1006..2679
                     /locus_tag="PKW60_10370"
     CDS             1006..2679
                     /locus_tag="PKW60_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HPJ99879.1"
                     /translation="MADSEGGASEQFRPSEPGHPAATLVSLYRRYGVDFVTRLRGHFA
                     LAIWDEPAEAFLLISDRFGMRPLYYLQNDTGIAFASEVKALLTLAPAPPPVDERGLCD
                     YILFGIPLDNRTFFTNIQCVPPASILTFRAGQPPTTRRYWELEFHDKSTAFHTIDDAA
                     RALKTALQDAVTESIEAEHPIELPLSGGLDTRCIAALAMGTRTTLRTYSLGSQNSEDL
                     RVGTVVAQQLGLPNQAWTLAPRDLMEWTEEAIYLTDGMYNPIDSPILYIARRLPGDAR
                     IAIDGASSFDGLYRFFDPFFFRLLPSRYAGTKMPMRALVAPVVRPDGNTIPQVLHPDY
                     EAFAREHALATLNALIESVPPANRSNPFDSLDYLDLRNRLPRCNVMGSVLLRSRCEVR
                     HPFFDPRVIDLVTCFKPFLRAKEKLAAGRLINLAAPELAALVYERTGIPADSPVVRHL
                     LAYGKTALGHATGRFLPFTATKPRVAIDFGSWVQHNPPLQSFIRTLLLDAPASRLPYF
                     DPGALETLLNRLFVGHTACLPLVGRMMSLELWHRYFVEGQSPPVLQAEV"
     gene            complement(2844..>4251)
                     /locus_tag="PKW60_10375"
     CDS             complement(2844..>4251)
                     /locus_tag="PKW60_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HPJ99880.1"
                     /translation="FKGRVIMIGCGSIGQATLPLVGRHLDHVYERMTVLSADESGRGI
                     AERCGAKFIHCNLTPGNFRATLKKYVGDGDLMMNLSVDICSIDLIRFCAEQGALYVDT
                     SIEPWPGVFDNPMLELHERTNYALRVRLLELAAELGPDSPTAVVDHGANPGLVSHFVK
                     RALFDLNRAIRDDNTKPSTREEWAVLARDLKITTIQVSERDTQASKRPKKQGEFINTW
                     SVDGFVDELVQPAELSVGTFEKRWPRGAREHSPGSGTLYLTRPGASTFARSWVPSIGG
                     FQGMLVTHDENFSIADYLSIRDGDGFQYRPSVMFVYHPCDDAMLSALELEGRGWAMQP
                     SSRRLRIDIVEGMDELGVLLTGHERNAYWYGSQLTIEEARRHVPYANATTIQVAAGAI
                     AATLWAIRNPRRGVLEPEDLDFDDCLKIALPYLGRMAGEFTEWTPLDGRGKYFPEKLD
                     VHNPWQLQNIRSRQWYGE"
BASE COUNT          840 a         1431 c         1132 g          848 t
ORIGIN      
        1 gtctctgccg cgaccacgtc cgatggtcag gccgtcactg gagacttctc ctgccggatt
       61 cgagcgatgc gttcttccag agcatgaaac gaaaacaccg caacgcgctg caaaaagcct
      121 gcgctctcat ggaccaggaa aataatgatg agggcttgag ctgggagcgt tttatccgcc
      181 cggaccaggt tgaccggttc tgccaggaag ctgaccagat cgcccggaag acctaccaac
      241 gccagctcgg tgcgagtttt gccacaaccg aatccaccct gaaaaagctt cggctcctgg
      301 ctgaacgcaa ccaatggcgg gcttatattc tgtacgtgca cggcgtaccc gccgcctact
      361 ggataggcac cagctacggc acaacgttcc atgtggaata caccggccgt gaccccgcct
      421 tcagccaatt caagcttggc actggcacca tgctctttgt gaagatgatt gaagacatct
      481 gcggcgcgac cgatatacgc gaagtcgatt tcgggttcgg cgactatccc tacaagcagc
      541 gctttggcaa cgtcaacgaa gaaacacaga ctctgttcct tttcgcgccg acattctcca
      601 acgtgagcct caatatgctc cgcaccgcac tggcatggac cgaaaaaggt ctggccgctc
      661 tcctggatcg ccttggcctc cgcgcacgga ttaatcggtt cagacgcgag cgcctgggcc
      721 gaacagaata agtatgagca gggcacgaca catccttttc atcgcgagga caagaatgac
      781 atgccaggtt tagctgggta cgtggcgaag cacggcgccg ccgaagaaca taccatccgt
      841 ggaatggctg cccctatggc ctgctcacgg cacgcccgga aaatggacac cgtatgtgcc
      901 ggacaggcag ccctcgcctg cgacgccaac gcctgaggac ctcagagccg ccgggtcatc
      961 gcggacgacg gcatcgtcct ggggatcgat ggcgaaatcc tcgctattgc ggactccgaa
     1021 ggcggcgcct cggaacaatt ccgccccagt gaacccgggc atcctgcggc gactctagtc
     1081 agtctgtacc gccgatacgg cgtcgacttc gtcacccgct tgcggggaca ctttgccctg
     1141 gcgatctggg acgagcctgc ggaggccttc ctgctcattt ccgaccggtt cggtatgcgg
     1201 cccctctact accttcaaaa cgacaccggc atcgcgtttg cttccgaggt gaaggccctg
     1261 ctcactctgg cgcctgcccc gcctcccgtg gatgaacggg gcctctgcga ctacatcctc
     1321 ttcggaattc ccctcgataa ccgaaccttc ttcacaaata tccagtgcgt tccgcctgcc
     1381 tccatcctca cgttccgcgc cggccagcca cccacaaccc gccggtactg ggagcttgaa
     1441 tttcacgata aatcaacggc gttccacacc atcgacgacg ccgcgcgcgc gctcaaaacc
     1501 gccctgcagg acgccgtcac ggaaagcatc gaagccgaac atcccattga actccccctg
     1561 agtggcgggc tcgacacccg ctgcatcgca gccctcgcca tgggaaccag aacgactctg
     1621 cggacctata gcctcggcag ccagaacagc gaagacctcc gcgtggggac agtcgtggcg
     1681 caacaactgg gcctccccaa tcaggcctgg acacttgcgc cccgggatct tatggaatgg
     1741 actgaggagg ccatttacct taccgatggc atgtacaatc ccatcgactc ccctatcctc
     1801 tatatcgcca ggcgccttcc tggcgatgca cgcatcgcta tcgacggcgc cagttcattc
     1861 gatgggcttt accggttctt tgacccgttc ttcttccgcc tgctcccttc gcgctacgcc
     1921 ggcacaaaaa tgcccatgag agcgctcgtt gcccccgttg tccgccccga tggaaacacg
     1981 atcccgcagg tactccatcc cgattacgaa gctttcgccc gcgagcacgc ccttgccacc
     2041 ctgaacgctc tcatcgaaag cgtgcctccc gcgaaccgga gcaacccatt cgacagcctg
     2101 gattacctcg atcttcgcaa tcgcctgccc cgctgcaatg tcatgggctc cgtcctgctc
     2161 cgctcccgct gcgaggtccg ccaccccttc ttcgatcccc gcgtcatcga cctcgtcacc
     2221 tgcttcaagc ccttcctgcg cgccaaagaa aagctcgcgg ccggacgcct catcaactta
     2281 gccgcacccg aactcgccgc gctcgtctac gaacgcacgg gcatccccgc tgactcccct
     2341 gtcgttcgcc acctgcttgc ctacggcaaa accgcactgg gccacgcaac cgggcgtttc
     2401 ctgccattta ctgccacaaa accccgcgtc gccatcgatt tcggctcctg ggtgcaacac
     2461 aacccgcctc ttcagtcgtt catccgcacc ctgctcctcg atgcgcccgc ttccaggctg
     2521 ccgtacttcg atcccggcgc gctcgagacc cttctcaacc gcctcttcgt cggccacacc
     2581 gcgtgcctcc ccctcgtcgg ccgcatgatg agtctcgaac tctggcaccg ctacttcgtg
     2641 gaaggccagt ccccaccggt tctgcaagct gaagtctgaa gcgtttgttg agtgcagcca
     2701 attcaacgcc acggtacacg gctacaggcc agtgaatccg ttctcacgca cttccgttcg
     2761 cgtcgaccac gcttgtgtgc tgtttcggcg attgggttct gcctgccagt ttgccggtgc
     2821 ggcggcatca ggccggccgc gcgtcattcg ccataccact gcctgctgcg aatattctgg
     2881 agctgccagg ggttgtgcac gtccagtttt tcggggaaat atttgccgcg gccatcgagc
     2941 ggggtccatt ccgtgaactc gccagccatg cgtccgagat aaggcagcgc tatcttgagg
     3001 caatcgtcga aatccagatc ttccggctca agcacgccgc gccgcggatt gcgtatggcc
     3061 catagagtgg cggcgatggc gccggccgca acctgtatcg ttgtcgcgtt cgcatagggc
     3121 acgtgccgcc gggcctcctc gatcgtgagc tgcgagccgt accagtaggc gtttctttcg
     3181 tggccggtca gcaatacacc aagctcatcc attccctcga cgatgtctat cctcagccgc
     3241 cggctggaag gctgcatcgc ccatcccctg ccttcgagtt ccagggcaga cagcatggcg
     3301 tcatcacacg ggtggtacac aaacatcaca ctgggccggt actggaagcc atcgccatcg
     3361 cgaatggaca gatagtcggc gatcgaaaaa ttttcgtcat gagtcacgag catcccctga
     3421 aaaccaccga tcgacggcac ccagctgcgc gcaaaagtgc tcgccccggg acgggtcaaa
     3481 tacagcgttc cggaaccggg ggaatgctca cgcgcccccc tgggccaccg cttttcgaag
     3541 gtgccgacgg acagttcggc cggttgtaca agttcgtcca caaaaccgtc cacggaccag
     3601 gtgttgatga attcaccttg ctttttcggc cgtttgctgg cctgtgtgtc gcgttccgag
     3661 acctgaatgg tggttatttt gagatcacga gcgagaaccg cccactcttc gcgcgtggag
     3721 ggttttgtgt tgtcatcgcg aatggcgcga ttcagatcga acagagcgcg tttgacgaaa
     3781 tgagagacca gccccggatt ggccccgtga tcaaccaccg ccgtgggtga atcaggcccc
     3841 agttcggcag caagttcgag taatcgcaca cgcagggcgt agttggtgcg ctcgtgcagt
     3901 tccagcatcg ggttgtcaaa cacgcccggc cagggttcga tcgaagtatc tacatataga
     3961 gcgccctgtt cggcgcagaa tcgaatcagg tcaatgctgc agatatcgac ggacaagttc
     4021 atcatgagat cgccgtcgcc aacgtatttc tttaatgtgg cgcgaaaatt gcccggcgtc
     4081 agattgcagt gaatgaattt cgctccgcag cgctccgcga tgcctcgccc cgattcgtca
     4141 gcggacagca ccgtcatccg ttcgtaaaca tggtcgagat gccggccgac aagcggcagc
     4201 gtcgcctggc cgatgctccc gcagccaatc atgatcacac gtcccttaaa g
//
LOCUS       DAOPBQ010000319         4249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17098_length_4249_cov_4.358846, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000319 DAOPBQ010000000
VERSION     DAOPBQ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17098_length_4249_cov_4.358846"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /gene="rpoB"
                     /locus_tag="PKW60_10380"
     CDS             <1..586
                     /gene="rpoB"
                     /locus_tag="PKW60_10380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824071.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPJ99881.1"
                     /translation="QLLESMLGWSARVLGMKVATPVFNGASESLIGDFMKKAGLPEDG
                     RVPLRDGRTGKPFDQLVTVGYLYMMKLNHLVDDKIHARAIGPYSLVTQQPLGGKAQFG
                     GQRFGEMEVWALQAYGAAYTLQELLTIKSDDIQGRTKAYEAVVKGDREVEPGTPESFN
                     VLVREMQSLCLDVIKLARVEDGTDADEDEEELEG"
     gene            590..>4249
                     /gene="rpoC"
                     /locus_tag="PKW60_10385"
     CDS             590..>4249
                     /gene="rpoC"
                     /locus_tag="PKW60_10385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPJ99882.1"
                     /translation="MAKYVPTTSERFDALQIRMASPEEIQKWSRGEVKKPETINYRTF
                     KPEKDGLFCERIFGPVKDWECGCGKYKKVKHRGITCDRCGVEITESKVRRERMGCIKL
                     AVPVTHIWFFKTTPSSIGNLLDLSIRVLERIIYFENYIVIDPGDTSLDKMKTLTEDEY
                     QDAREEWGDRFVAKMGAEAIKMLLEEIDIDALSAELHSQVTATNSMQARAKAIKRLKV
                     VEALRKSGNNPAWMVLDVVPVIPPDLRPLVPLEGGRYATSDLNDLYRRVINRNNRLKR
                     LIELRAPEVILRNEKRMLQEAVDALFDNGRHGRTVLGAGNRPLKSLSDMLKGKQGRFR
                     QNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFEPFIIRELKERGIASTIKAA
                     KRTIAMGREEVWDILDEVIKDHPVLLNRAPTLHRLGIQAFEPVLIEGKAIRIHPLVCA
                     AFNADFDGDQMAVHVPLMPVAQLEARLLMLSSTNIFSPSTGRPIATPSQDIVLGIAYI
                     TKARIGARGEWQDTWTGPDGQILNPGKIYPDTESVLVAYDVGEVDLHARIKVWYEGVV
                     VDTTVGRVIFKESLPAGITLNDVNQEQTQSTIGDIIAKSYLEHGHNKTVALLDALKQT
                     GFKYATLAGLSIGVRDFAVPEVKRELIQRANDSVARIDEMYQNGLITEGERYNKVIDE
                     WTTATEALTAAMLKELEKNEQGFSGINLMLKSGARGNKQQIRQLAAMRGLMAKPSGDI
                     IESPITSNFREGLTVLEYFISSHGARKGLADTALKTADAGYLTRRLVDVAQDVVIEED
                     DCGTLNGVWVEALMDGGEVVQPLEERIVGRFPLDDVTVPGESEPLVSADEEIDETKAH
                     AIAQKGIPGLRIRSVLTCASKRGVCARCYGRNLATGRLVEMGEAVGIIAAQSIGEPGT
                     QLTMRTFHIGGAASRQVESSEERVAEEGMLEFRNVRTAKNRDDKLVVVNRGGEVALLD
                     DEGKEIQRYAVPSGALLHAENGAKVKKGRLLYEADPYNVSIVAEATGTVHLEGMVEGI
                     TMRKDINPDTGLEERIVTDHKQDLHPQITILGQEDEVLAYATIPGQTHVLVADGAKVQ
                     AGDLLAKTPKQFSKVSDITGGLPRVAELFEARQPKDPAVISHIDGTVELGGAAKGMRK
                     VRIIPPVGKEREYTVLPGKHLNVQAGDRVYAGQRLTDGPVIPQDILEVQGEDALRKYL
                     LDEIQQVYRLQGVRTN"
BASE COUNT          914 a         1137 c         1483 g          715 t
ORIGIN      
        1 ccagctgctc gaatcgatgc tcgggtggtc ggcgcgcgtg ctcggcatga aggtggcgac
       61 gccggtattc aacggcgcct cggaaagcct gatcggcgat ttcatgaaga aggccggcct
      121 gccggaagac ggaagagtgc ccctgcgcga tggccggacc gggaaaccgt tcgatcagct
      181 cgtgacggtc ggctatttgt acatgatgaa gttgaaccac ctggtggacg acaagattca
      241 cgcgcgcgcg atcggtccct actcgctggt cacgcagcag ccgctgggcg gcaaggcgca
      301 gtttggcggg cagcgtttcg gcgaaatgga ggtatgggcg ctccaggcct atggcgcggc
      361 gtacacgctt caggagctgc tgaccatcaa gtcggatgac attcagggac gaaccaaagc
      421 gtacgaggcc gtggtgaaag gcgatcggga agtggagccg ggaacgccgg aatcgtttaa
      481 tgttttggtg cgtgagatgc agtcgctttg tctcgatgtg atcaagctgg cacgcgttga
      541 ggatgggacc gacgcggacg aagacgaaga agagctggag ggctaaacct tggccaagta
      601 tgttccgacg acgagtgaac ggtttgatgc gctgcagatc cgcatggcgt ctccggagga
      661 gatccagaag tggtcccgcg gagaggtgaa gaagcccgag accatcaact accggacgtt
      721 taagcccgag aaggacggtc tgttctgcga acgcattttt ggcccggtga aggactggga
      781 atgcggttgc gggaagtaca agaaagtgaa gcaccggggc atcacgtgcg atcgttgcgg
      841 cgtggagatt acggagtcga aggtgcggcg cgaacggatg gggtgcatca agctggcggt
      901 gccggtgacc cacatctggt ttttcaagac gacgcccagc tcgatcggca atctgctgga
      961 tctgtcgatc cgggtcctgg agcggatcat ctatttcgaa aattacatcg tcatcgatcc
     1021 gggcgacacg agcctcgaca agatgaagac cctcaccgag gacgagtatc aggacgcacg
     1081 cgaggaatgg ggcgatcgct ttgtggccaa gatgggcgcg gaagcgatca aaatgctcct
     1141 ggaagagatc gacatcgatg ccctgagcgc ggagctgcac agccaggtca ccgccacgaa
     1201 ctcgatgcag gcgcgggcca aagcgatcaa gcgcctgaag gtcgtggagg cgctgcgcaa
     1261 atcggggaac aacccggcct ggatggtcct ggatgtggtg ccggtgattc cgccggatct
     1321 gcgccccctg gttccgctgg aaggcggccg ctatgcgacg agcgacctga acgatctgta
     1381 ccggcgcgtg atcaaccgaa acaaccgttt gaagcgcctg atcgagctgc gcgccccgga
     1441 agtgatcctc cgcaatgaaa agcggatgct tcaggaagcc gtggatgcgc tgtttgacaa
     1501 cggccgccac ggacggacgg tgctcggcgc ggggaaccgg ccgctgaaat cgctgagcga
     1561 catgctgaag ggcaagcagg gacggttccg ccagaatctg ctcgggaaac gcgtggacta
     1621 ttccgggcgt tccgtgattg tggtgggccc tgagctcaaa ctgcatcagt gcggcctgcc
     1681 gaagaagatg gcgctggaac tgttcgagcc gttcatcatc cgggagctga aggagcgggg
     1741 cattgcctcg acaatcaaag cggccaaacg gacgatcgcc atgggccgcg aagaggtctg
     1801 ggacatcctt gacgaagtga tcaaggacca tccggtgctc ttgaaccgcg cgccgacgct
     1861 gcaccgcctg ggcattcagg cgtttgaacc cgtgctgatc gagggcaagg cgatccgcat
     1921 tcatccgctg gtgtgcgcgg cattcaacgc ggactttgac ggcgaccaga tggcggtgca
     1981 cgtgccgctt atgccggtgg cgcagcttga ggcccgcctg ttgatgcttt cgtcgacaaa
     2041 catcttctcg ccatcgacgg gccgtccgat cgccacgccc agccaggaca tcgtcctggg
     2101 catcgcgtac attaccaagg cgcgcattgg ggcgcgcggc gaatggcagg acacctggac
     2161 cggcccggac ggtcaaatcc tcaatccggg aaaaatttat ccggacacag agtcggtgct
     2221 ggtggcgtac gatgtgggcg aggtggatct tcacgcgcgc atcaaggtgt ggtacgaggg
     2281 cgtcgtggtc gataccacgg tcggccgggt gatcttcaaa gagtcgcttc cggcaggcat
     2341 cacgctgaac gatgtgaacc aggagcaaac ccagagcacg atcggcgaca tcattgcgaa
     2401 atcgtatctc gagcatgggc acaacaaaac ggtggccctg ctcgatgcac tgaagcagac
     2461 gggattcaag tatgccacgc tggcgggcct ttctatcggc gtgcgcgact ttgcagtgcc
     2521 ggaggtcaaa cgcgagctga tccagcgcgc caatgactcg gttgcgcgca tcgacgagat
     2581 gtatcagaac ggcctgatca cggaaggcga gcgttacaac aaggtcattg acgagtggac
     2641 cacggcgacc gaagcgctca cggcggcgat gctgaaggag ctggagaaaa acgagcaggg
     2701 tttcagcgga atcaacctga tgctgaaatc aggcgcgcgc gggaacaagc agcagattcg
     2761 ccagctggca gcgatgcgcg gactgatggc caagccgtcg ggcgacatta ttgagtcgcc
     2821 gatcacgtcg aatttccgcg aagggctgac ggtattggaa tacttcattt cttcgcatgg
     2881 cgcgcgcaaa ggccttgcgg ataccgcgct gaaaacagcg gacgcgggtt atctcacgcg
     2941 gcgcctggtg gacgtggcgc aggacgtggt cattgaagag gacgactgcg gcacgctgaa
     3001 cggcgtgtgg gtggaagccc tgatggacgg cggcgaagtg gtgcagccgc tggaagaacg
     3061 catcgtgggc cgctttcctt tggatgatgt cacggtgccg ggggagagcg agcccctcgt
     3121 gagtgccgat gaagagatcg atgagacgaa ggcgcacgcc atcgcccaga agggcattcc
     3181 gggtttgcgc atccggtcgg tgctgacctg tgcgtcgaaa cggggcgtat gcgcgcggtg
     3241 ctatgggcgc aacctggcaa cgggccggct ggtggagatg ggcgaagcgg tgggcatcat
     3301 cgcggcgcag tccatcggtg aaccgggcac gcagttgacc atgcgcacgt tccatatcgg
     3361 cggcgcggca agccggcagg tggaatcgag tgaagaacgc gttgcggaag agggcatgct
     3421 cgaattccgc aacgtgcgca cggcgaagaa ccgggacgac aagctggtcg tggtgaaccg
     3481 gggcggtgaa gtcgccctgc tggacgatga agggaaggag atccagcggt atgccgtgcc
     3541 gtcgggcgcg ttgctgcacg cagaaaacgg cgccaaggtg aagaagggcc ggctgctgta
     3601 cgaagcggat ccctacaacg tgtcgatcgt ggcggaggcc acggggacgg tgcacctgga
     3661 aggcatggtt gaaggcataa cgatgcgcaa agacatcaac ccggacacgg ggctcgaaga
     3721 acgcatcgtt acggatcaca aacaggatct tcacccgcag attacaattc tcgggcagga
     3781 agacgaagtg ctggcgtacg cgacgatccc ggggcagacg catgtgctgg tcgcagacgg
     3841 agcgaaagtg caggcgggtg atctgctggc gaaaaccccg aagcaattca gcaaggtgtc
     3901 ggatattacg ggcggcctgc cccgcgtagc ggaattgttt gaggcgcggc agccgaaaga
     3961 cccggcggtg atcagccaca ttgacggtac ggtggaactg ggtggcgcgg cgaagggcat
     4021 gcgcaaggtc cggatcattc ctcccgtggg caaggaacgg gagtacacgg tgctgccggg
     4081 caagcacctg aacgtgcagg cgggcgaccg cgtgtacgcg ggccagcggt tgaccgacgg
     4141 cccggtgatc ccgcaggaca tcctggaggt gcagggcgaa gatgcgcttc gcaagtacct
     4201 ccttgacgag attcagcagg tgtaccgcct gcagggcgtg cggacgaat
//
LOCUS       DAOPBQ010000320         4246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17117_length_4246_cov_3.955381, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000320 DAOPBQ010000000
VERSION     DAOPBQ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17117_length_4246_cov_3.955381"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="PKW60_10390"
     CDS             complement(<1..981)
                     /locus_tag="PKW60_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HPJ99883.1"
                     /translation="MSDMGDKSQLDDDAGPYRSTRAKVLGVTAAVLLLALVCCGLPAA
                     SAAGGASGGDTGQTTGLADHLPAPVVALVVILLGLSAFFSGSETAFFSIHRLRLRVIA
                     EEQTLSGRFITTMMQTPGRLLTTILVGNTMVNVLISVLLGTRVQLYFERTFGWSRPVA
                     YPAAVAACTAVLVFFGEISPKIVAVRAAEPLARIVALPLSLADRILAPLRNGLLRLTD
                     LIFRATRFNEIHAAPFITDEELKAALSTGESHGAIEKGERQMIQGILEFTDTRLREVL
                     TPRPDVVSLSFSATVQEALDAYREHEYSRMPVFEDDMDHIRGVIVMKDLLP"
     gene            complement(978..1481)
                     /gene="ybeY"
                     /locus_tag="PKW60_10395"
     CDS             complement(978..1481)
                     /gene="ybeY"
                     /locus_tag="PKW60_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPJ99884.1"
                     /translation="MSQDRPLAVHLDVINQSERKRLYRTDALRRLAERVCAGEGLTGN
                     VEVSVLFCDDSRITDLNRDYRNREQPTDVLSFSQAGEFSPNLRILGDIVISLETVERH
                     CRGGRDAMRQEIRLLFCHGLLHLLNYTHSDALTRRAMTKKQALYLDIPLKNAWPGMPK
                     THAANPA"
     gene            complement(1478..4006)
                     /locus_tag="PKW60_10400"
     CDS             complement(1478..4006)
                     /locus_tag="PKW60_10400"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPJ99885.1"
                     /translation="MIFRPHGKKRYRAGIPFRPQDKRVGLAKWIPRKSRWALLASFLV
                     LSAALSRYVESIPLALLEGMDYKALAPDAIQADIAFDAPDPEATRLARAEASAKVPDT
                     FRVNRERVQEQLAAFDRFAASVLSQRKGSDAALRETLLASDSSQKMEDLVWATAVARA
                     RTLLKGESAPPDLLPEDAAVLLLPDRRSIPDREFEGVTEWGVPAPGQPPRPAVALKDK
                     TPAAGSLSFTAADDLLEMTRVALEYGLQFGILSPEARGTVLEGEKQPDIKIVRDSPMP
                     DQARSEVFAPKEVPKPDSSRNLLSIRLAQALQGDSSPAAELPVERSKALAGAVAVADA
                     FTTATLEFDSVGTASEREEAAAKIEPKTLHVPRRHIIINAREEWTEAAVAKYEAYLAA
                     KRARRPMQRYLVELGARMVLVALVLACLLQALRAVRDRRADPPQQTNVTLLIMCFTVV
                     AGTVISISAPSGFVVPAAAGAILLAILINTRVAMVGSLLTTVLLSIAYEYSWGLFLVQ
                     SAMCLAGVFSIRVVRRRSDMTRASVKATVVGLITVSAVLLATGDLLTEQALRTVLLIG
                     LNGLLCLFLVPGLLSPLERIFGITTDIQLLEYSDLNNEILSRMAIEIPATNAHSQRLG
                     QLAEAAADAIGANGLLAQVCAYYHDIGKLRRPEYFVENQAGANIHDELTPRLSARAIA
                     SHVLEGAEIAREMHLPKPIVDGILEHHGTCLISFFYQEALAQQKHGDVREEDFRYPGP
                     KPQSRETAILMICDASESGVRSIKNPNEERIREFVDKIVKARADDGQFDECDLTLKDL
                     DTIKEIVTSRLVSFAHQRIVYPDQKEPPQPKAPNVVALRGRYES"
     gene            complement(4003..>4246)
                     /locus_tag="PKW60_10405"
     CDS             complement(4003..>4246)
                     /locus_tag="PKW60_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016821588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPJ99886.1"
                     /translation="DITQIDLPRGVKSGLVEARSILKGVTGIGIVHLTRGDVVRHALV
                     QEIIAAYERAEGRAGTEDDHAARGGEEISRVRGGRA"
BASE COUNT          778 a         1314 c         1366 g          788 t
ORIGIN      
        1 ggggaggagg tctttcatga cgatgacgcc gcggatgtgg tccatatcat cctcaaacac
       61 gggcatccgg gagtattcgt gttcgcggta ggcgtcgagg gcttcctgca ctgtcgccga
      121 gaacgaaagc gacacgacat cgggccgggg cgtcaacact tcgcgcaacc gggtatcggt
      181 aaactcgaga atcccctgga tcatctggcg ctcacccttt tcgatagcgc catgggattc
      241 gccggtcgac agcgcagcct tgagttcttc gtcggtgatg aacggtgcgg catggatttc
      301 attgaaccgg gtggcgcgaa agatgaggtc ggtcaggcgc agcagcccgt tacgcagggg
      361 cgccaggatg cggtcggcca ggctgagcgg cagcgccacg atccgggcca ggggttcggc
      421 ggcccgcacg gctacgatct tgggcgagat ctcgccgaaa aacaccagta ccgccgtaca
      481 tgcggccaca gccgcgggat acgccaccgg ccgactccat ccgaacgtgc gttcgaaata
      541 cagttgaacc cgcgtgccca gcagcaccga gatcagaacg ttgaccatcg tgttgcccac
      601 caggatggtc gtgagcaaac ggccgggcgt ctgcatcatc gtggtgatga atcgcccgga
      661 gagggtctgt tcttccgcga tcacgcgcag gcgcaagcga tgaatggaga agaaagccgt
      721 ctctgaaccc gagaagaagg ccgacaagcc cagcagtatc acaaccaagg ccaccacagg
      781 ggcaggcagg tggtccgcca ggcccgttgt ctgcccggta tccccgccgg aggctccgcc
      841 cgcggcagag gccgccggca gcccgcaaca aacgagcgcc agaagcagta cggctgccgt
      901 cacgccgagc acctttgcgc gtgtacttcg gtaaggcccc gcgtcatcgt ccaattgcga
      961 cttgtctccc atgtcactca tgccggattt gccgcatgtg tcttcggcat gcccggccat
     1021 gcgtttttga gggggatatc caggtagagc gcctgttttt tcgtcatggc gcgccgcgtc
     1081 aacgcatcgg aatgcgtgta attcaacaga tgcagcaagc catggcaaaa gagcagccgt
     1141 atttcttgac gcatcgcatc gcgcccgccc cggcaatgcc gctccacagt ctccaatgat
     1201 atcacgatat cgcccaaaat ccgaagattg gggctgaatt ctccagcctg gctgaatgag
     1261 agcacgtccg tcggctgctc ccggtttcgg taatcccggt tcaggtccgt gatgcgcgag
     1321 tcatcgcaga aaagcacgct cacctcgacg ttcccggtga ggccttcccc tgcgcagact
     1381 cgttctgcga gacggcgcag cgcgtccgtg cggtacaggc gtttgcgctc ggattggttg
     1441 atcacgtcca gatggacggc cagcggcctg tcctggctca cgactcatac ctcccgcgaa
     1501 gggccacgac attgggcgcc ttgggctgag ggggttcctt ctggtcggga tacacgatgc
     1561 gctgatgcgc aaagctcacc agacgcgagg tcacgatttc cttgatggta tcgagatctt
     1621 tcagggtgag gtcgcattcg tcgaactggc cgtcgtcagc gcgggctttg acgattttgt
     1681 cgacgaattc gcggattcgc tcctcattgg gatttttgat cgagcgcacc ccggattcgg
     1741 aggcatcgca gatcatgagg atggcggttt cgcggctctg cggttttggc ccgggatagc
     1801 ggaaatcttc ttcgcgcacg tcgccgtgct tctgttgcgc cagggcttcc tgatagaaaa
     1861 agctgatcag gcaggtgccg tggtgttcca ggatgccgtc cacaatcggt ttcggcaggt
     1921 gcatttcgcg ggctatttcc gcaccttcca ggacatgcga ggcaatggcg cgcgcgctca
     1981 gccggggcgt caactcatcg tggatgttgg cgccggcctg attttccacg aaatactccg
     2041 gccggcgcag ctttccgatg tcgtgataat acgcgcagac ttgcgccagc aggccgttgg
     2101 cgccgatagc atcggcggcg gcttccgcca gttgtcccag gcgctggctg tgcgcgttgg
     2161 tggcaggtat ctcaatggcc atgcggctga ggatctcgtt gttgaggtcg gagtattcga
     2221 gcaattggat gtcggtggtg atgccgaaga tgcgctcaag gggcgagagc agtccaggca
     2281 cgagaaacag gcagagcagg ccgttcaagc cgatcaggag aaccgtccgc agcgcctgtt
     2341 ccgtaaggag gtcgccggtg gccaggagca ccgcggacac ggtgatgagc ccgaccacgg
     2401 tggctttgac cgaagcgcgg gtcatgtcgc tgcggcggcg caccacccgg atgctgaaca
     2461 cgcccgccag gcacatggcg ctctggacca ggaacaggcc ccagctgtat tcataggcga
     2521 tagacaacag gacggtcgtc agcaggctgc cgaccatggc cacacgcgtg ttgatcagga
     2581 tggcgagcaa aatggcgccc gcggcggccg gcacgacaaa acctgacggg gccgagatgg
     2641 aaatgaccgt accggccacc acagtaaaac acatgatcag cagcgtgacg ttggtctgct
     2701 ggggcggatc ggcgcgccgg tcgcgcaccg cgcgcaacgc ctgcaagagg caggcgagca
     2761 ccagcgccac cagcaccatg cgcgcgccca gttcaacgag atagcgctgc atcggccggc
     2821 gcgcgcgttt tgcggccaga taggcctcgt atttcgcgac agccgcttcc gtccattcct
     2881 cgcgggcatt gatgatgatg tgcctgcgag gcacatgcag cgtcttgggt tcgatttttg
     2941 cggcggcttc ctcgcgttcc gaagccgttc cgacgctgtc gaattccagt gtggcggtcg
     3001 tgaaggcgtc cgcgacggcc actgcgccgg ccagggcctt tgatcgttcc acgggcagtt
     3061 cggctgcggg gcttgaatcg ccctgcaatg cctgcgccag gcggatgctg agcagattgc
     3121 gcgacgaatc gggtttgggg acttccttgg gcgcgaagac ctcgctgcgc gcctgatcgg
     3181 gcatcggcga atcgcgcaca atcttgatat cgggctgttt ttcgccctca agcacggtgc
     3241 cccgagcctc aggcgacagg atgccgaact gaagcccgta ttcgagcgcg acgcgcgtca
     3301 tttcaagcaa atcgtccgcg gcagtaaacg agaggctgcc cgcggcgggc gtcttgtctt
     3361 tcaaggcaac agccggccgg ggcggctgcc ccggggccgg aacgccccat tcggtcacgc
     3421 cttcaaactc gcggtcagga atggaacggc ggtcagggag cagcaaaacg gcggcatcct
     3481 cgggcaggag atccggcggg gcgctttcac ctttcagcag tgtccgggcg cgcgcaactg
     3541 cggtggccca gaccaggtct tccatcttct gggaggagtc agaggccagc agtgtctcgc
     3601 gcagcgcggc atccgagcct ttccgctggc tcagcacgct cgccgcgaac cggtcgaatg
     3661 cggccagctg ctcctgcacg cgctcgcggt ttacacgaaa ggtgtccggg acttttgccg
     3721 aagcctccgc ccgggccagg cgggttgctt caggatccgg tgcgtcgaac gcaatatccg
     3781 cctgaatcgc gtccggcgca agggctttgt aatccatccc ttccagcagc gcaagcggaa
     3841 tggattccac atagcgcgac agtgccgcac tgagcaccag aaacgatgcc agcagcgccc
     3901 accggctctt tcggggtatc catttggcca gaccgacgcg tttgtcctgt ggccggaagg
     3961 ggataccggc tcgataccgc ttctttccat gcggacgaaa gatcatgcgc ggcccccccg
     4021 gacgcgcgag atctcttcac cgccgcgcgc ggcgtggtcg tcctccgtcc cggcgcgtcc
     4081 ttccgcacgt tcgtacgcgg caatgatctc ctgcaccagc gcgtggcgga ccacatcgcc
     4141 gcgggtcagg tggacaatgc cgataccggt gacgcctttg aggatgcttc gcgcctcgac
     4201 caggcccgac ttgacccccc ggggcaggtc gatctgcgtg atgtcg
//
LOCUS       DAOPBQ010000321         4236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17156_length_4236_cov_3.313083, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000321 DAOPBQ010000000
VERSION     DAOPBQ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17156_length_4236_cov_3.313083"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /gene="infA"
                     /locus_tag="PKW60_10410"
     CDS             <1..99
                     /gene="infA"
                     /locus_tag="PKW60_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002816015.1"
                     /note="stimulates the activities of the other two
                     initiation factors, IF-2 and IF-3; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPJ99887.1"
                     /translation="RKYFIRILPGDRVKLEMSPYDLSKGRVTYRFK"
     gene            complement(242..1000)
                     /locus_tag="PKW60_10415"
     CDS             complement(242..1000)
                     /locus_tag="PKW60_10415"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HPJ99888.1"
                     /translation="MRAANILVFFGAICVALVFPAAAEGDTPSDSKPLRAVVLTGGHG
                     YDKEAFEAMFRGVEGIEFVFQEQRDHSELFEDISGWDYDVIVFYSMTQEISEKRQQNL
                     LALLDRGVGVVALHHIISGFNQWPEFQKIIGSRFLFAPVEQDGTTFPVSSYKHDITVN
                     VTIADPAHPVTKGVTGFTAVDETYLGCMFQPDNQVLLTTDEPTSDKTIGWVRTYRKSR
                     VCTIQLGHGPTIFADPMYKKLVMQAVRWTARKTG"
     gene            complement(1078..2526)
                     /locus_tag="PKW60_10420"
     CDS             complement(1078..2526)
                     /locus_tag="PKW60_10420"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HPJ99889.1"
                     /translation="MRAAEAIAWISLVMAFPAWAGEEPVSPARDEAAAALQRAVEFFD
                     GTVSVEGGYLWRYAADLVLREGEGKASASQAWTQPPGTPSVGMALLRAYDYTGEAYLL
                     EAARRTAHALVKGQLLSGGWQYSIEFEPAKRSRYAYRVDSAEGEGQNTSTLDDDTTQA
                     ALRCLMLADKALEFSDEGIHGAVEYGLEQLLAVQYPNGAWPQRFSGPRDPEAFPVKPA
                     SYPDEWSRTYEGKDYQSYYTFNDNTIGDTIETMFLAAELYGRGDCRDSAARAGDFILL
                     AQMPLPQPGWAQQYDADMHPAWARKFEPAAITGGESQGVMRTLLYLYKKTGEERFLEP
                     LPRALAYYEASTLPDGRLARFYELRTNTPLYFTLDYKLTYSSDDMPTHYSFIVGSTLP
                     SIREEYERLKEIPPEDLQAPSSVPKPAKPRLTPSLAQNAARVLAALDERGAWIQAGDL
                     RYHEPGSAERVIDCSTFIKNVGILAEFLAATE"
     gene            2755..3483
                     /locus_tag="PKW60_10425"
     CDS             2755..3483
                     /locus_tag="PKW60_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPJ99890.1"
                     /translation="MHYAIISDIHGNLEALTAAFESIDHRQVSHIVCLGDVVGYGASP
                     DECVNLIRERGIPTILGNHDAVACGLEEPWGFNQVALSAVKWTAEQLSPENITWLRSL
                     PPRLEYESFLAVHATPEGIGWDYIFSWEETLPYIDWLREQKRRLCFFGHTHCPGAFSE
                     DGLYALEEDSRFELDPGKVYLINPGSVGQPRDDDPRASLGIFDSETNEFELVRVPYAV
                     DVAAKRILDAGLPGFLAERLHRGR"
     gene            3696..>4236
                     /locus_tag="PKW60_10430"
     CDS             3696..>4236
                     /locus_tag="PKW60_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPJ99891.1"
                     /translation="MASSLAGAVALGWGAKLRAQEPRPDRWVLLADIHIPADRDQANG
                     KPPIKPVEKFAAMRSEVLALDYRPDGVIIAGDCVYLHGQHEDYVTLKGEVQPFVDAGI
                     PLHLSMGNHDNREQFWEVFAEHQKYSANTVEGKHAYILDAPRATWFVLDSNRETNYTP
                     GEFGEAQLEWLANELDQRPD"
BASE COUNT          816 a         1339 c         1227 g          854 t
ORIGIN      
        1 cgcaaatatt tcatccgcat tctgcccgga gaccgtgtca aactcgaaat gtcgccatac
       61 gacctcagta agggacgtgt cacgtaccgg ttcaagtaga ccggcctgcc tcgctccggc
      121 ccgcggccag cgggcgagac tcctgtctgt actcacgcgt gccagtacac gcgccgtttc
      181 gtctcccgcc gtacgccgga gctgactccc cgcacagctt tagcccggac gtggattccg
      241 ctcaaccggt ttttcgtgcc gtccagcgca ccgcctgcat cacaagtttc ttgtacatgg
      301 ggtctgcaaa aatggtcggt ccgtgtccca gttgaatggt gcagacccgc gacttccggt
      361 acgtgcgcac ccagccgatc gtcttgtcgc tggtgggctc gtctgtggtg agcagcacct
      421 ggttgtccgg ctggaacatg cagcccagat atgtctcatc caccgccgtg aatcctgtaa
      481 ccccttttgt tacggggtgg gcagggtccg cgatcgtgac attgaccgtg atgtcgtgtt
      541 tgtaggaaga aacgggaaaa gtcgtgccgt cctgttcgac cggcgcaaac aaaaagcgcg
      601 atccgatgat cttctgaaac tcaggccatt gattgaaccc ggaaataatg tggtgcagcg
      661 ccacaacccc caccccgcgg tccagcaggg ccaggagatt ctgctgccgt ttctccgaga
      721 tctcttgcgt catcgaataa aacacgatga cgtcgtagtc ccaaccggag atatcttcaa
      781 acaattcgct gtggtcgcgc tgttcctgaa agacgaactc aatcccttcg accccccgga
      841 acatcgcctc aaacgcctct ttgtcgtaac cgtggccacc cgtaagaacc acagcccgca
      901 acggctttga gtcagaaggc gtatcgccct ccgccgcggc cggaaaaacc agcgctacac
      961 aaatcgcccc aaagaacacc agaatattcg ctgctcgcat gactttgctc ccttcccgtg
     1021 tccatcgtct acgatcgagt caatccagcc ttcaggccgc tgcctctttc gattccatta
     1081 ttccgtcgcc gccagaaatt ccgccagaat cccaacgttc ttaatgaacg tcgaacaatc
     1141 gatcacccgc tcggccgaac ctggctcgtg atagcgcaga tcgcccgcct ggatccaggc
     1201 gccgcgctcg tccagcgccg caaggaccct ggccgcgttt tgagccagac tgggcgtcag
     1261 acggggtttt gctggtttgg gcaccgagga gggcgcctgc agatcctccg gcggaatctc
     1321 tttgaggcgc tcgtattctt cccgaatgga cggcagcgtt gatcccacaa tgaatgaata
     1381 gtgcgtgggc atgtcatcgc tgctgtacgt gagtttgtaa tccagggtga aatacagggg
     1441 cgtattggtc cgcagttcat aaaagcgtgc caggcggcca tctgggagcg tggacgcctc
     1501 gtaataggcc agagcccgcg gcaaaggctc gagaaagcgc tcttcaccgg tcttcttgta
     1561 gagatacaag agtgtgcgca tcaccccttg cgactccccg ccggtaatgg cggctggctc
     1621 gaacttgcgg gcccaggcgg ggtgcatatc ggcatcgtat tgttgagccc agcccggctg
     1681 gggcaacggc atctgcgcga gaaggatgaa gtcccctgcc cgtgccgcgg agtcgcggca
     1741 gtcgccccgg ccgtaaagct ctgccgccag aaacatcgtc tcgatagtat cgccgatcgt
     1801 gttgtcgttg aacgtgtaat aactctggta atcctttcct tcgtaggtcc gtgaccattc
     1861 atcgggatag gatgcgggtt tgacgggaaa cgcctcggga tcccgcggcc cgctgaaccg
     1921 ctgcggccag gctccgttcg ggtactgcac cgccagcagc tgttcgaggc cgtactcgac
     1981 tgcgccgtga atcccctcat ccgaaaattc gagtgctttg tcggccaaca tgaggcagcg
     2041 caatgcggcc tgggttgtgt catcgtccag ggtgctggtg ttctggccct cgccttcagc
     2101 cgaatccacg cggtaggcat agcggctgcg ttttgcgggc tcaaactcga tcgaatactg
     2161 ccatcctcca gagagtaact ggcctttcac cagcgcatgg gcggttctcc gtgccgcttc
     2221 gagcagatag gcctctcccg tgtagtcata ggcccggagc agggccatgc ccacgctcgg
     2281 cgtgcccgga ggctgcgtcc atgcttgaga cgcgctcgcc ttgccttccc cctcacgcaa
     2341 caccaggtcg gccgcgtacc gccacaggta cccgccctca accgaaacgg tgccatcgaa
     2401 gaattcgacg gcgcgctgca aggctgcggc tgcctcgtcg cgagccggag agaccggctc
     2461 ctcgcccgcc caggccggga atgccattac cagggaaatc catgctatcg cctcagctgc
     2521 tctcatcagg actcctctcg cggaacggtg tgaacggcgg tcaggttgca ctttcaggcg
     2581 catccatgca ttcttatcgc agttccagca ggagtgcgtc aaattcctgc ccgccgcgcg
     2641 cgtttgcagg cgggcggcgc atgggcgcgc tccacaggca cccgcagcag gccgcaaggc
     2701 ttcgaagaag gcgctggccg gtggacaggc caggtgaaga ggggggcagt gcgcgtgcac
     2761 tacgccatta tttcggacat ccacggcaat ctggaggctc ttaccgctgc cttcgaatcc
     2821 atcgaccacc gccaggtctc ccacatcgtc tgcctcggcg atgtcgtcgg ctatggcgct
     2881 tcgccggatg aatgcgtcaa cctgattcgt gagcgcggca tcccgaccat cctcggaaat
     2941 cacgacgcgg tcgcctgcgg cctggaagaa ccgtgggggt tcaatcaggt ggcgctctcc
     3001 gcggtcaagt ggacggccga gcagctcagt cctgaaaata tcacctggct gaggagtctt
     3061 ccaccgcgcc tggagtacga atccttcctc gccgtccacg ccacgccgga aggcatcggc
     3121 tgggattata ttttctcgtg ggaggagacc ctgccctata tcgactggct ccgcgaacag
     3181 aagcgccggc tgtgcttttt cgggcacact cactgccccg gcgccttttc cgaagacggc
     3241 ctctacgcgc tggaagagga ttcccggttc gagcttgatc caggcaaggt ctacctgatt
     3301 aaccccggct cggtgggaca accccgtgat gacgatcccc gggcctctct tggcattttc
     3361 gatagcgaaa cgaatgaatt cgagctggtc cgcgtgccgt atgcggtcga tgtggctgca
     3421 aaacgcattc tggatgccgg tcttcccgga tttctcgccg aacggctgca ccggggccgt
     3481 taagccacaa accggcaggc acaccacgtg tctccaggcg ccctgccggc cacaagtgcc
     3541 tcggtgcacc agcccgcccg gccattgggg caaaggccgg tgtggatgat ttgtgcccgg
     3601 ttttgaggta tccttctggg agaaacttat tcactggaca catcgtcagg aggttggttg
     3661 tgaacacata ccgtcgcatt tccagaagac acttcatggc atcgtctctt gcgggtgcgg
     3721 tcgcgcttgg atggggagca aagctccgcg cccaggaacc ccgccccgat cgatgggtgc
     3781 tgctggctga tattcatatc cccgcggacc gcgaccaggc caacggcaaa ccgcccatta
     3841 aaccggtcga gaaattcgcg gcgatgcgtt ccgaagtcct cgcgctggat taccggccag
     3901 acggtgtcat catcgcgggg gactgcgtct atctccacgg ccagcacgag gattacgtca
     3961 cactcaaagg cgaagtgcag ccgtttgtgg acgccggcat ccccctgcac ctgtccatgg
     4021 gcaaccacga caaccgcgaa cagttctggg aggtatttgc ggagcatcag aagtacagcg
     4081 cgaatactgt cgaggggaag catgcctaca tcctggacgc gccgcgcgcc acgtggtttg
     4141 tgctcgattc caatcgcgaa accaattaca cgcccgggga atttggtgag gcccagctgg
     4201 aatggctggc gaacgaactc gatcagcgcc cggaca
//
LOCUS       DAOPBQ010000322         4235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17160_length_4235_cov_4.710526, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000322 DAOPBQ010000000
VERSION     DAOPBQ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17160_length_4235_cov_4.710526"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PKW60_10435"
     CDS             <1..1218
                     /locus_tag="PKW60_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HPJ99892.1"
                     /translation="AALVEAAISAAETPPVEERAAEDTPPETPAETKAVAQSFEARLQ
                     ALAPGESLKTAAASLLRAWDMALLADVPAGGGVQDLKQFAQAYGLTGEPLPAGLPELE
                     AVNLPAFVRMSSGGKTLWVALLSTGAESVRCSMEPGETMDVPRGEFVQAYRAESVVLW
                     RDPAPGAETLKPDMTGDQVRALQHRLRQLGRYQGELTGIYDAATSQAVTGIQAATALK
                     RDGKAGQQVRMVLCSWLDDIPTPELHAMERRPPVQMASEPPPAAKPEAPPAPPELVQA
                     KKAKPAPLPAPAPAPELDLPKAPESATTEAEKAGTEPLSEEAPEAAGDVMAAETPQNE
                     TAQLVTVMELPADAPAASPAAGRDVEALLTEEVYGQSSGETGKEVTSPVATGAPLVPH
                     QGTTARTADGDNG"
     gene            1215..2258
                     /locus_tag="PKW60_10440"
     CDS             1215..2258
                     /locus_tag="PKW60_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99893.1"
                     /translation="MSSILDALNKAEEERVAQEYALSRAFDDLDHGDIEDELTGRANG
                     ARGRGAGMPLTPLRIAMLAAAFVAVVAAASGGAAWMVFHLRTEPAPVFPTESAGLSLA
                     GVATASPTLPELAPQVTAHPAPVELSSASPNPAPEAIISSSTPAPLPIPSPAETVMQE
                     TSAPAPPAERESAAAALPEEAGSAAPEPRDAPSAPELSEPRVQVAAAEPPRPAAAPKH
                     APAAPAQPESEWATPPPAAAVATVPEPARAAKTVPRDAPPATKRVEAGEVDILALPEL
                     TASDRRRLSLPDITVNVVGRPSKYRPQPSAMINFSRVVLNEYIPGTQAQLIGVSVHGI
                     GIQVGGERYFVPK"
     gene            complement(2271..2990)
                     /locus_tag="PKW60_10445"
     CDS             complement(2271..2990)
                     /locus_tag="PKW60_10445"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPJ99894.1"
                     /translation="MTMRLQRYLALCGVASRRASESLIREGRVTVNGAIAVLGATVDP
                     DSDEIRVNGKPIRRERPAYILLHKPKGTITTVDDPQGRKTVLDCLQGIRARVYPVGRL
                     DFDVSGVLLFTNDGELAQRLTHPRYGVTKVYIATVRGLVSDEALRRLETGVALADGIT
                     APAQAAVLARSRDTTRLRLTLHEGRKREVKRMCAEVGHPVVTLRRTHFAGIEAGSLRP
                     GQWRRLEPAEVAQLRRLVKLS"
     gene            complement(3086..3790)
                     /gene="scpB"
                     /locus_tag="PKW60_10450"
     CDS             complement(3086..3790)
                     /gene="scpB"
                     /locus_tag="PKW60_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPJ99895.1"
                     /translation="MMTEEDIQGLEPLDSLRETEERERAAADESAALREEDDAVEIDG
                     GAPADEDGELEPVEQLGRDETRAVLHALLFVSDKPTSVDRLSEALGGVDYDVVRNLLA
                     ELREEIEDLSLPYVLREIAGGYQLATRSEYAPYIRRMFQIKRRNRLSKAVLETLAIIA
                     YKQPCTRADVESVRGVSVSHAFETLREKRLIKVAGVAETVGRPKLYRTTDEFLAHFGI
                     KSLKELPTIEELRETV"
     gene            complement(3807..>4235)
                     /locus_tag="PKW60_10455"
     CDS             complement(3807..>4235)
                     /locus_tag="PKW60_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPJ99896.1"
                     /translation="AVGKARKPVFLKRGMMSTISELLMSAEYIMAEGNPQVILCERGI
                     RTFERETRNTLDISAVPVLKKYSHLPVIVDPSHATGHWELVAPMARAAVAAGADGIMV
                     EVHPHPADAFSDGAQSLKPGKFKKLMAELKPLVAAMGKTL"
BASE COUNT          751 a         1325 c         1458 g          701 t
ORIGIN      
        1 gctgcattgg tggaagcggc gatcagcgcg gccgaaacgc cgcccgtcga agagcgtgcg
       61 gcagaggata caccccctga gacgccggct gagacgaaag ccgtggcgca gagtttcgag
      121 gcgcggctcc aggcgctggc tcccggcgaa agtctgaaaa cggcggcggc aagcctgctt
      181 cgtgcatggg acatggcgct gctggctgat gtgccagcgg gtggcggagt gcaggacctc
      241 aaacagttcg cgcaggccta tggcctgacc ggcgagccgc tgccggcggg attgccggag
      301 ctggaggccg tgaaccttcc cgcgtttgtg cggatgagct caggcgggaa gaccctctgg
      361 gtggccctgc tttccacggg ggccgagtcg gttcgatgct caatggagcc aggcgagacc
      421 atggacgtgc cgcgcggcga gtttgtgcag gcctaccggg cggagtcggt ggttctctgg
      481 cgcgacccgg cgccaggcgc cgaaacgttg aagcccgaca tgacgggcga ccaggtgcgc
      541 gcgctccagc accgtttgcg ccagctgggg cggtaccagg gcgaattgac gggaatatac
      601 gacgccgcga cctcgcaggc cgtgaccggg atccaggcag ccacggcgtt gaaacgtgac
      661 ggtaaagcag gccagcaggt gcgcatggtc ctgtgcagct ggctggacga tatcccaacc
      721 cccgagctcc acgcgatgga gcggcggcct ccggtacaga tggcgagcga accgccacca
      781 gccgcgaagc ccgaggcgcc gccggccccg ccggagcttg tacaggctaa aaaagccaag
      841 ccagcgccac tgcctgcccc cgcgcctgcg cccgaactgg acttgccgaa agcaccggaa
      901 tccgctacaa cagaagcgga gaaggccggc acggagcccc tttcggaaga ggcgcctgaa
      961 gccgccgggg atgtcatggc ggcggagacg ccccagaacg aaaccgcgca actggtgacg
     1021 gtgatggaat tgcccgcaga cgctccagcc gcgtcccccg cagccgggcg tgacgtggaa
     1081 gcgctgctga cagaagaagt ctatgggcaa agctccgggg agaccgggaa ggaggtaacg
     1141 tcaccggtgg ccacaggggc gccgctcgtc ccacatcagg gaaccacggc caggacggcg
     1201 gatggggata acggatgagt tcgattctcg atgcgctgaa caaggcggaa gaggagcggg
     1261 ttgcccagga atatgcgttg tcgcgcgcgt ttgacgacct cgaccatgga gatattgagg
     1321 acgagttgac cgggcgcgcg aacggcgccc gcgggcgggg tgccggcatg ccgctgacgc
     1381 cgctccggat agcgatgctc gcagcggcct ttgtggcagt cgtcgcggcg gcgtcgggcg
     1441 gcgccgcatg gatggtgttt catctgcgga ccgaaccggc gccggtgttc cccaccgaat
     1501 ctgccgggct gtcactggcg ggcgttgcaa ctgcgtctcc gaccctgccg gagctggcgc
     1561 cgcaggtcac ggcgcatccg gcgcccgttg aattgagttc tgcctcgccg aacccggctc
     1621 ctgaggcaat catctcctcc agcacgccgg caccactgcc catcccatca ccggcagaga
     1681 ccgtaatgca ggagacttca gcgcctgcgc cacccgccga gcgcgagagt gctgccgcgg
     1741 ccctgccgga agaggcaggg agcgctgcgc ccgagcccag ggacgcgccg tcggcgccgg
     1801 agctgtctga accccgcgtg caggtggctg cagcagaacc tccacgcccc gccgctgcac
     1861 ccaagcatgc gcccgcggct ccggcgcagc cggagtcgga atgggcaacc ccgcctcccg
     1921 ccgcagcggt ggccacagtt ccagagcccg ctcgcgcggc caagacggtg ccgcgagacg
     1981 cgccgccggc caccaaacgg gtcgaagccg gggaagtaga catactggcg ttgccggaac
     2041 tgacggcgtc ggaccggcgg cgcctgagcc tgccggatat tacggtgaat gtcgtgggga
     2101 gaccgtcaaa ataccgcccg cagccgtcgg ccatgatcaa tttcagccgc gtggtcttga
     2161 atgagtacat cccggggacc caggcgcagt tgatcggggt gagcgtgcac ggcatcggca
     2221 ttcaggtagg cggcgaacgc tattttgtgc cgaagtgacg ctccggccat tcaggaaagc
     2281 ttcaccagac ggcggagctg tgccacctcg gcgggctcca gccgccgcca ctgcccgggg
     2341 cgcagcgaac ccgcctcgat gcccgcgaaa tgggtcctcc gtaacgtgac gacaggatgc
     2401 ccgacttccg cgcacatgcg tttcacctcg cgtttccgcc cctcgtgcag ggtaagacgg
     2461 agacgggtgg tgtctcggct gcgggccaga acagctgcct gagcgggcgc ggtgatgcca
     2521 tccgcgaggg ccaccccggt ttcgagcctg cggagggcct catcggagac gagaccccgg
     2581 acggtagcga tatacacttt ggtgacgccg taacgcgggt gggtgagccg ctgagcaagc
     2641 tcgccgtcgt tggtgaacag gaggacgccg ctgacgtcga agtcgaggcg tccgacgggg
     2701 tatacacggg cgcggatccc ctggaggcaa tccagcacgg tcttgcgccc ctgcggatca
     2761 tcgaccgtgg tgatggtccc tttgggcttg tgaagcaaaa tgtaggcggg ccgctcgcgg
     2821 cggatgggtt tgccgttaac gcgaatttcg tcggaatccg gatcgacggt agcgccaagc
     2881 acagcaatgg cgccgttgac ggtcacgcgg ccctcccgga tgagagattc ggaagcacgg
     2941 cgcgacgcga cgccgcagag ggccaggtaa cgttgaaggc gcatggtcat cggatgtgcc
     3001 tcgcaggcgg ctctggccgc agtctcaagc gtctcacaga gactccttcg cgggaaaacg
     3061 gacacggcgc cgaacgctcc gcccgctaga cggtttcgcg caattcctcg atggtcggaa
     3121 gctctttgag gctcttgatg ccgaaatggg cgagaaactc atccgtggta cggtatagtt
     3181 tcggccggcc tacggtttcg gcgacgcccg cgaccttgat gagccgcttc tcgcggaggg
     3241 tctcgaaggc atgggagacg ctgactcccc ggacgctttc gacatccgcg cgggtgcagg
     3301 gctgtttgta cgcaatgatg gccagggttt caagaacggc cttggaaagg cggttgcgcc
     3361 gtttgatctg aaacatgcgg cggatatagg gggcgtactc gctgcgcgtt gccagctggt
     3421 acccgcccgc gatttcgcgc agcacatacg gcagagacag gtcttcgatc tcttcgcgca
     3481 gctccgcgag gaggttgcgg actacatcat agtcgacccc gccgagcgct tcagagagcc
     3541 ggtcgacact ggttggtttg tcggagacaa acagcagggc gtgcagcacg gcccgggttt
     3601 cgtcgcgtcc aagctgctcc acgggctcaa gctcgccatc ttcgtcagcg ggtgcgccgc
     3661 catcgatttc gacggcatcg tcctcttcgc ggagagccgc ggattcgtcg gcggcggctc
     3721 gctcgcgttc ttcggtttcg cggaggctgt cgagaggttc caggccctga atgtcttctt
     3781 ctgtcatcat ggttgtgggt cagtggttac agtgttttac ccatggcggc caccaggggt
     3841 ttcagctcgg ccatgagctt tttaaacttt ccgggtttca atgactgggc accgtcggag
     3901 aaggcgtcgg caggatgcgg atgcacctcc accataatgc cgtcggcgcc ggctgcaaca
     3961 gcggccctcg ccatgggagc caccaattcc cagtgcccgg tggcatggct cggatctacg
     4021 attacgggca ggtggctgta cttcttcagc accggcaccg cgctgatgtc gagcgtgttg
     4081 cgggtttccc tttcgaaggt gcgtatgcca cgttcgcaca gaatgacctg cgggttccct
     4141 tccgccatga tgtattccgc cgacatgaga agctcgctga tggtggacat catgccgcgt
     4201 ttgagaaaga caggtttacg ggctttgccc acagc
//
LOCUS       DAOPBQ010000323         4235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17162_length_4235_cov_4.203110, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000323 DAOPBQ010000000
VERSION     DAOPBQ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17162_length_4235_cov_4.203110"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="PKW60_10460"
     CDS             <1..139
                     /locus_tag="PKW60_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPJ99897.1"
                     /translation="PLWIEVNTIPGMTETSLFPQAAAAAGIAFPELLDRLIRGARKQA
                     H"
     gene            212..1054
                     /gene="dapF"
                     /locus_tag="PKW60_10465"
     CDS             212..1054
                     /gene="dapF"
                     /locus_tag="PKW60_10465"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPJ99898.1"
                     /translation="MTIEFTKMHGLGNDYLFIDAQHCPVDNPGALSRAMSHRHLGAGA
                     DGIILVLPGESGADFAMRIFNADGSEAETCGNGVRCFAKYVFERGMTHKTEMVILTGA
                     GPNRVSLTLDDGVVQSVRSNMGSPRFDRSEIPMIGPPGRVIEEKLAIPGQTFTITCVN
                     IGNPHTVIFVDDATAVPLAELGPKIENHPSFPQRTNVEFVTVRDRENIVMRIWERGSG
                     ITMASGSGSCASALAAMITERTARRVNVHLVYGELAVEWADDGFVYQEGPATEVYVGQ
                     WPED"
     gene            1089..1712
                     /gene="purN"
                     /locus_tag="PKW60_10470"
     CDS             1089..1712
                     /gene="purN"
                     /locus_tag="PKW60_10470"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPJ99899.1"
                     /translation="MPLKIAVLLSGSGTTLQNLIDRAGSGELDVQFVCVIGSRTGAYG
                     LERARKHDIPAIPIVRKDYGSLDEFNAAIWDTVRRHGAELVVLAGFMSLLQIPGDYEN
                     RVINVHPALIPAFCGKGMYGHHVHEAVIEYGVKLSGATVHFANNIYDAGPIILQDAVP
                     VLDNDTPDTLAERVQAKERELYPKAIRLFAEGRLRVEGRRVRILDPQ"
     gene            complement(1744..2724)
                     /locus_tag="PKW60_10475"
     CDS             complement(1744..2724)
                     /locus_tag="PKW60_10475"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPJ99900.1"
                     /translation="MLRARITGTGAFLPETVWTNAKLESMMDTSDEWIRKRTGIGQRH
                     LAAAGEGSSDLGAPAARDALVNAGVAPEDVELIICCTTTPDYLFPATACLIQDRIGAR
                     NAGAFDLNAACTGFVSGLATANAYIRAGVYRTVLVVGTEAQTNRLNWAKRETAVLFGD
                     GAGATLLQAEDGDRGVLSTFLGADGSDGDMLIIPGGGSKAPLTKANMDGSGLDIEMKG
                     AELFKRATVLFEEASMKALESARLRLDDVDLFVPHQANARIIGAAAKRLGLPEEKVVL
                     NLERVANTTSASIPLALHEAVVKGRVKDGDLLLLAGFGAGLTWGAAVIRW"
     gene            3292..4137
                     /locus_tag="PKW60_10480"
     CDS             3292..4137
                     /locus_tag="PKW60_10480"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HPJ99901.1"
                     /translation="MKRDDVMPDQLVRPQKAYKNLSFLNSPDARAIRVLCEFLEPQAR
                     FDRMNVEHTVVFFGSARTLPESEASAQCQKAKEALELAQGDMEALEQEYLQAQRALTM
                     SRYYEDAAALSEKLTRWSLTLSDPRDRFVVCSGGGPGIMEAANRGAARAGGDSVGLNI
                     SLPHEQYPNPYQTPELAFEFHYFFIRKFWFVHLSRALIAFPGGFGTFDELFELVTLLQ
                     TRKTPNNRCVILYGSEFWNEVVDFDALVRWGVISPEDLDLYHMYDDVDETFEHLKAHL
                     TKHHL"
     gene            4173..>4235
                     /locus_tag="PKW60_10485"
     CDS             4173..>4235
                     /locus_tag="PKW60_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPJ99902.1"
                     /translation="MKGIILAGGSGTRLYPITMAV"
BASE COUNT          827 a         1359 c         1257 g          792 t
ORIGIN      
        1 gcccttatgg atcgaggtca acaccattcc cgggatgaca gagacatcgc tgtttcccca
       61 ggcagccgcg gcggcaggca ttgcctttcc tgaactcctg gaccgcctga ttcgcggggc
      121 caggaaacag gcgcactaat cacgccatct tcacggcggc ccttctgtcc cggggtggcg
      181 tctcgacccg cgcacgcata aaggaaacgc catgaccatt gaattcacca agatgcacgg
      241 cctgggcaac gattacctct tcatcgatgc gcagcactgc ccggtcgaca accccggcgc
      301 gctctcccgc gccatgagcc accggcacct cggtgcgggc gccgacggca tcattctcgt
      361 gcttcccggc gaaagcggcg ccgattttgc catgcgcatc ttcaatgccg acggcagcga
      421 ggccgaaacc tgcggcaacg gcgtccgttg ctttgccaaa tatgtctttg agcgcggcat
      481 gacccacaaa accgagatgg tcatcctgac gggcgcaggg cccaaccgcg tttcgctgac
      541 cctcgacgac ggcgtggttc aatcggtccg ctccaacatg ggttcgcccc gcttcgatcg
      601 gtctgaaatc cccatgatcg gccctccagg ccgggttatc gaggaaaaac tcgccatccc
      661 cggccagacc ttcaccataa cctgcgtcaa tatcggcaat ccgcacacgg tcattttcgt
      721 cgatgacgcc acggccgtcc ccctggccga gctcggcccg aaaatcgaaa atcacccgtc
      781 gtttccccag cgcaccaacg tcgaattcgt gaccgtgcgc gaccgcgaaa acatcgtcat
      841 gcggatctgg gaacgcggca gcggcatcac catggcgagc ggcagcggtt cctgcgcatc
      901 cgcgctcgca gccatgatca ccgagcgcac cgcgcggcgc gtcaacgtcc acctggtcta
      961 cggcgagctt gccgtggaat gggcagacga cgggtttgtg tatcaggaag ggccggccac
     1021 ggaggtctac gtcggccaat ggccggaaga ttgaccacgc gcagtccatc cggacgggaa
     1081 agcttgccat gccactgaaa atcgcggtac tgctttcagg cagcggcacc acgctacaga
     1141 atctcattga tcgtgccggt tccggcgaac tcgacgtgca gttcgtttgc gtgatcggct
     1201 cccgcaccgg cgcctacggc ctcgaacgcg cccgaaaaca cgatatcccg gccattccca
     1261 tcgtccgcaa ggattacggt tctctcgacg agttcaatgc cgcgatctgg gacaccgtcc
     1321 gccgtcacgg cgcggaactg gtcgtgctcg ccgggttcat gagcctcctg cagatccccg
     1381 gcgactatga gaaccgggtc atcaacgtcc atcccgccct gatccccgcg ttctgcggaa
     1441 aaggcatgta tggacaccat gttcacgagg ccgtcatcga atacggcgtc aaactctccg
     1501 gcgccacggt gcatttcgcc aacaacatct acgacgccgg gcccatcatc ctgcaggatg
     1561 cggttcccgt gctcgacaac gacacgccgg ataccctggc cgagcgggtg caggccaaag
     1621 aacgcgagct ctatcccaaa gccatccgcc tcttcgcgga agggcggctt cgcgtcgaag
     1681 gccgccgcgt ccgcattctc gatcctcaat aggcgctccg gcctgggctc ctgtccggcg
     1741 tcatcaccag cggataacgg ccgcgcccca ggtcagtccg gcgccaaaac cggcgagcaa
     1801 cagcagatcg ccatccttga cccggccttt gaccaccgcc tcatgcaagg ccagcgggat
     1861 cgaggccgag gtcgtgttgg ccacgcgctc gaggttcagg acgaccttct cttccggcag
     1921 ccccagacgt tttgccgccg cgccgataat ccgcgcattc gcctgatgcg gcacgaacag
     1981 atcgacgtca tccaggcgca atctcgctga ttccagagct ttcatcgacg cttcctcaaa
     2041 aagcaccgtg gcccgtttga acagctccgc gcctttcatc tcgatgtcca atccggaccc
     2101 gtccatattc gctttggtca gtggggcttt cgagccgccc ccagggatga tcagcatatc
     2161 gccatcggag ccatcggccc ccagaaacgt cgacaagact ccacggtcgc cgtcttcggc
     2221 ctgcaagagc gtcgccccgg cgccgtcacc aaacaagacc gccgtctcgc gtttggccca
     2281 gttcagccgg tttgtctgcg cctccgtgcc taccaccagc accgtgcggt agacgcccgc
     2341 gcggatgtag gcgttcgccg tcgccagacc gctgacaaac ccggtacacg cagcattcag
     2401 atcgaacgcg cccgcgtttc gtgcgccaat gcgatcctgt atcaggcagg ccgttgccgg
     2461 aaacaggtaa tcgggagtcg ttgtgcagca aatgatcagt tccacatctt ccggggctac
     2521 tccggcattg accagcgcat cccgcgctgc cggtgcgccc aaatcggacg acccctcgcc
     2581 cgcagccgcc agatggcgct gcccaatccc cgtgcgcttt cgaatccact cgtccgaagt
     2641 atccatcatc gactcaagtt tcgcgttggt ccagaccgtc tccgggagaa acgcgcccgt
     2701 gcctgtaatt cgtgctcgca gcattaaagt gttctcctgt aagccacacg aggtccccgc
     2761 cctcaagaaa gcatacggac aggcttcatg cccccaagca ttgcgtgaag atcgtccatc
     2821 agccgggttg cgggatccgg tatagctcct gccacagcag tgggtgcccc tcaaagcgca
     2881 aagcataccc caggcagcgg atggctctca atgtttcgcc gcgggaaagc gagggcagga
     2941 ccccaaaacc atggccgccc ctgcaacccg gagggcgacg gagggttgcc ctgcgccacg
     3001 cctctcatgc ccggcatgaa agcaaagggt gtggccaata caccttccgg catcagtcgc
     3061 cgccgcctgt cgatttcgca gggcagcctg gcagcgcgga gacggtgcgt ttgacccatc
     3121 ggcggcatca acgtacattt gtgacgaatt ttgtgtgcgg tttatccggg cagggggcgc
     3181 tttccagagg gccggcacgc cggcctgccc ggctttccgg ggcttccggg ttgcggcggg
     3241 gcggcaaaac gtgtacaaaa gggtctcgct acgaagaaca ggatgcccat tatgaaacgt
     3301 gacgatgtga tgccggatca attggtgcgc ccacagaaag cgtacaagaa tctcagtttt
     3361 ctgaacagtc cggatgcccg ggccatacgg gtattgtgcg agtttctcga gccgcaggcg
     3421 cgttttgacc ggatgaatgt agagcacacc gtggtgtttt tcggttcggc gcggaccctg
     3481 ccggagagcg aagcgtcggc gcagtgtcag aaggcgaagg aagcgctcga actggcgcaa
     3541 ggagacatgg aggcgctcga acaggaatat cttcaggcgc agcgggcgct taccatgtcg
     3601 cggtattacg aagatgcggc ggcgctgtcg gaaaagctga cgcggtggtc gttgaccttg
     3661 tccgatccgc gggatcggtt cgtggtgtgc tcgggagggg gcccggggat catggaagcg
     3721 gccaatcggg gcgcggcacg ggcgggcgga gactcagtcg ggctgaacat cagcctgccg
     3781 cacgaacagt atccgaatcc gtatcagaca ccggaactgg cgttcgaatt tcactatttc
     3841 tttatccgga aattctggtt tgtgcatctt tcgcgggcgt tgatagcgtt tcccggcggt
     3901 tttgggacct ttgacgagct gtttgagctg gtcacgctgc tgcagacccg aaagacgccg
     3961 aacaaccgct gcgtgatctt gtatgggtcg gagttctgga acgaagtcgt ggattttgat
     4021 gccctggtgc gatggggggt catcagtccc gaagatctgg atctgtacca catgtatgat
     4081 gacgtggacg agacgttcga gcatttgaag gcccatctga cgaagcatca tctgtaggcg
     4141 ggcggcggtc tgagggaagg gcgaggaaag tcatgaaggg aatcatcctg gcgggcggct
     4201 ccggaacgcg tttgtatccg atcacgatgg cggtc
//
LOCUS       DAOPBQ010000324         4227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17205_length_4227_cov_3.800815, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000324 DAOPBQ010000000
VERSION     DAOPBQ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17205_length_4227_cov_3.800815"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2325)
                     /locus_tag="PKW60_10490"
     CDS             complement(<1..2325)
                     /locus_tag="PKW60_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99903.1"
                     /translation="MKTVIAFQSDTLRAGVFWMCLVCAAVALAGESGPLYAKHDTWPE
                     TMTAARQAFQAAGGDPALELGPWFTTGALQAEGFAQALFPEHGVELEAKTADGQAAWT
                     PKPDWKDGVVINLPGGDSHSTYLYRTIQAASPVTLTASLGSDDGLEVWFNGEKLLSND
                     VPRVAAPNQDRVALSLNAGENRLLLKIFNRTGGHAFYFALTDPRLDTVWRQMAADFPD
                     QTQCFEQSAGSDGVWDWFRQTSTATEERIATDTLGRLGELAEGLKNELEALKTQQVAA
                     SDPRWLALFERAHRAAEHAERLNLVNLEALRRAIDDLRATYPDRYPQGDALSTRLAAL
                     EGSVQQARQTLRDARPGAENALGEAAAQAAALQRDALLANPLLDFDQLLLIKRGEGNL
                     GLPQNWQGNCALARAGYDNEICVMSPVTPDGALTTLFRPDGSRFVGDVDLHFDGSRML
                     ISMPGAHDRWQIWELNADGTGLRQVTPGAYPDVDNYDACYLPDGRIIFASTRCFQGIP
                     CVAGADAVANLCIMHPDGTNIRMLCFDQDHNWCPTVLNNGRVLFSRWEYSDTPHYFSR
                     LLFHMNPDGTNQMEYYGSNSFWPNSIFYARPIPGHPTKVVAIVSGHHGVARMGELTVF
                     DPAVGRREADGAVQRIPGYGESVDPVIADQLVADSWPKFLHPYPLNDKYFLVSCKPSP
                     EALWGIYLADIFDNLVLLKEEPGFALLEPVPFRATPAPPAIPDKVRLNSKEATVYLAD
                     IYEGDGLKDIPRGTVKKLRLYAMHYTYNKMGGHMH"
     gene            complement(2450..3103)
                     /locus_tag="PKW60_10495"
     CDS             complement(2450..3103)
                     /locus_tag="PKW60_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99904.1"
                     /translation="MTRRQMIGGVVAGTAMAAVGGRTRASEVSMLENKDFYKADGTFD
                     AAAAKEAYYDMMRRFNYPIVDRLQGEEFWAVDFGLGKFTEVGMAGIFWLNVKEHDFFG
                     HEIYLLPGQMIPEHRHLKTEDARPKMEGWHTRHGFVWTYGEGEPNPGDVERIPPSHRD
                     IAKARRALKLMPGEVTWLPEAEQWHSMLAGPEGAIVTEYASYHDNAALRFTIPNAKL"
     gene            complement(3189..4082)
                     /gene="lsrF"
                     /locus_tag="PKW60_10500"
     CDS             complement(3189..4082)
                     /gene="lsrF"
                     /locus_tag="PKW60_10500"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HPJ99905.1"
                     /translation="MADADKTTGDKKKNFGIGIPQETPGFFLKGSAHLDWGMKHRLAR
                     IFNPKSGRTVMLAFDHGYIMGPTSGIERIDLAIPPLIDYVDCLMCTRGALRTCIPPET
                     NKPVVLRCSTGATVLRELSDEVIGVTVEDAVRMNAAAVTTQVCIGAVHERETLENLSY
                     LINEGNNYGVPTLGVTAVGKELVRDARYLGLACRVIAELGAHFVKTYYCEPDFEEVVA
                     GCPVPIVIAGGKKLPEKEALDMAYKAINQGAAGVDMGRNIFCADDPVAMVQAVRQVVH
                     ENDTPDQAFDLYETLKREHAK"
     gene            complement(4075..>4227)
                     /locus_tag="PKW60_10505"
     CDS             complement(4075..>4227)
                     /locus_tag="PKW60_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HPJ99906.1"
                     /translation="RAGAEGTSALQARFGRAKNIGEKSIGQPDAGATSVSLLFKGFLE
                     GVTSHG"
BASE COUNT          727 a         1381 c         1291 g          828 t
ORIGIN      
        1 gtgcatgtgc ccgcccattt tattgtaggt atagtgcatg gcatagagcc ggagtttctt
       61 gacggtcccg cgcggtatgt ctttcagccc gtccccctcg tagatgtccg cgagatagac
      121 cgtggcctcc ttcgaattaa ggcgcacctt gtccgggatc gcgggcggcg ccggcgtggc
      181 ccggaagggc accggctcca gcagcgcgaa tccgggctcc tccttgagca gcaccaggtt
      241 gtcaaagatg tcggcgagat agatccccca cagggcctcg ggggacggtt tgcacgacac
      301 gagaaaatac ttgtcgttca ggggatacgg gtgcaggaat ttcggccagg aatccgcaac
      361 aagctggtcg gcaatgaccg gatcgacgga ctcgccgtag ccggggatgc gctgcaccgc
      421 gccatccgcc tcgcgccgtc ccacggcggg gtcgaatacc gtgagctcgc ccatgcgggc
      481 cacgccgtga tggccggaaa caatggcaac gaccttggtc gggtggcccg gtatcggccg
      541 ggcatagaaa atcgagtttg gccagaacga gttgctgccg taatactcca tctgattggt
      601 gccgtccgga ttcatgtgga acagcaggcg cgagaagtag tggggcgtat ccgagtattc
      661 ccaccgggaa aacaggaccc ggccgttgtt gagcaccgtc gggcaccagt tgtggtcctg
      721 atcaaaacag agcatgcgga tattcgtgcc gtcgggatgc atgatgcaca gattggccac
      781 ggcatcggcc cctgccacac aggggatccc ctggaagcac cgggtcgatg caaagatgat
      841 ccggccgtct ggaaggtagc acgcgtcata attgtccaca tcgggatacg cgcccggcgt
      901 gacctggcgg agcccggtgc cgtcggcgtt caactcccag atttgccagc ggtcatgcgc
      961 ccccggcatg gagatcagca tcctgctgcc gtcgaaatgc aggtccacgt cgcccacaaa
     1021 gcgcgaacca tcgggcctga agagcgtagt gagggcgccg tcgggcgtaa ccgggctcat
     1081 gacgcagatc tcgttgtcgt agccggcgcg cgcaagcgcg cagttgccct gccagttctg
     1141 cgggaggccc agattgccct ccccgcgttt gatcagcagc agctggtcaa aatcgagcaa
     1201 ggggtttgcg agcagcgcat cgcgttgcag cgcagcggcc tgagcagccg cttcgcccag
     1261 ggcattttcc gccccgggcc gggcgtcacg cagggtctgg cgcgcctgct ggaccgagcc
     1321 ttccaacgcg gccaaccgtg tcgacagcgc gtcgccctgg ggataccggt cgggataggt
     1381 tgcccggaga tcgtcgatcg cccgccgcag cgcctccaga ttgacaagat tcagccgttc
     1441 cgcatgctct gcggctcgat gtgcgcgctc gaacaaggcc agccagcgcg gatcgctggc
     1501 cgcaacctgc tgcgttttca gggcctccag ctcgtttttc agtccctcgg ccagttcgcc
     1561 caaacgcccc agggtatcgg tggcaatacg ctcctccgtt gccgtagaag tctggcggaa
     1621 ccagtcccag acgccgtcgc ttccggcgct ctgctcgaag cactgcgtct ggtctggaaa
     1681 atcggcggcc atctggcgcc agaccgtatc aagccgggga tcagtgagcg caaagtagaa
     1741 ggcatgtccc ccggtccggt taaaaatctt cagaagcagc cggttctccc cggcgttcag
     1801 cgagagggcc acccgatcct ggttgggggc ggccacccgc ggcacgtcgt tggaaaggag
     1861 cttctcgccg ttaaaccaaa cctccaggcc gtcatcgctt ccaagactgg ccgtcagcgt
     1921 caccggactg gccgcctgga tcgtgcggta caggtaggtg gagtgcgagt cgcccccggg
     1981 cagattgatg accacgccgt ccttccagtc cggcttgggt gtccaggctg cctggccgtc
     2041 agcggtcttc gcctccagct cgactccgtg ttcggggaac agggcctggg caaagccttc
     2101 cgcctgcagc gcgcccgtgg tgaaccacgg tccgagttcg agagcggggt cgccgcctgc
     2161 cgcctggaat gcctgccgcg cggcggtcat ggtctccggc cacgtgtcgt gcttagcgta
     2221 cagggggccg ctttcaccgg ccagcgccac ggccgcgcag accaggcaca tccaaaacac
     2281 tcccgcccgc aaggtatcgg attgaaaagc gatcacggtt ttcactgtcc ttttcttgtg
     2341 cggccgcctc tccccccgca gccggaggtt caggcgcccg cgcgcacaat ctcaatcctg
     2401 caccgccccg ccgggcggga tctggcccct gtccgcgccg actttcagtc tacagtttcg
     2461 cgttcgggat ggtgaaccga agcgcggcgt tgtcgtggta cgacgcatac tcggtcacga
     2521 tggcgccttc gggccctgcc agcatggaat gccactgttc ggcctccggc agccaggtca
     2581 cttcgccggg catcagtttg agcgcgcgcc gggctttggc gatatcacgg tgactgggcg
     2641 gaatccgctc gacatctccc ggattgggct cgccttcgcc gtaggtccag acgaacccgt
     2701 ggcgggtgtg ccagccctcc attttcggcc gcgcatcttc ggttttcagg tggcggtgtt
     2761 cggggatcat ctggccgggc aacaggtaga tctcgtggcc gaaaaagtcg tgttccttga
     2821 cattgagcca gaaaatgccc gccatgccca cttcggtaaa cttgcccagg ccgaaatcga
     2881 cggcccagaa ctcctcgccc tggagccggt cgacgatcgg gtaattgaag cgccgcatca
     2941 tgtcgtagta cgcctctttc gccgctgcag cgtcaaaggt accatccgct ttgtaaaaat
     3001 ctttgttctc cagcatgctc acctcgcttg cgcgtgtgcg gccgccaacg gccgccattg
     3061 ccgtgcccgc cacaacgccg ccaatcattt gccgtctggt catgtgactc atcacccggt
     3121 tcctcctggc ttcctccgcc tgcgcactgc ggcccctctg ccttcaatcg gtccggacgc
     3181 atggacgctc atttcgcgtg ttcacgtttc agagtctcgt acaggtcaaa cgcctggtcc
     3241 ggcgtgtcgt tttcgtgcac cacctggcgc acggcctgca ccatcgcaac gggatcgtca
     3301 gcgcagaaga tgttgcggcc catgtcgacg cccgcggcgc cctggttaat cgccttgtac
     3361 gccatgtcga gggcctcttt ctccggcagt ttcttgccgc ccgcgatcac aatgggaacg
     3421 gggcagcccg ccacgacctc ctcgaaatcg ggttcgcagt agtacgtctt cacgaaatgt
     3481 gccccgagtt ccgcgatcac ccggcacgcc agccccagat accgggcatc gcgcacgagt
     3541 tccttgccca cggccgtgac gccaagcgtc ggaacgccgt agttgttgcc ttcattgatc
     3601 aggtacgaca ggttttcgag cgtttcgcgc tcgtgtaccg cgccgataca gacctgcgtg
     3661 gtcaccgcgg ccgcattcat gcgcacagca tcttcgaccg tcactccgat cacttcatcg
     3721 ctgagctctc tgagcacagt cgcgcccgtg ctgcaccgca ggaccaccgg tttattggtc
     3781 tcgggcggga tgcaggtacg caacgcgccg cgggtgcaca tcaggcagtc aacgtagtca
     3841 atcagcggcg ggatagccag gtcgatccgt tcgatgccgg aggtcggccc catgatgtag
     3901 ccgtgatcga acgccagcat gacggtgcgc ccgcttttgg gattgaaaat gcgcgcaagc
     3961 cggtgtttca tcccccagtc cagatgggcg ctgcccttga ggaagaatcc gggtgtctcc
     4021 tgaggtatgc cgatcccgaa attcttcttt ttgtcgccgg tagttttgtc cgcatcagcc
     4081 atgtgatgtc actccttcca gaaatccctt gaacaagagc gaaaccgagg ttgccccggc
     4141 gtcgggttgt ccaatgctct tttcgccaat gtttttggcg cggccaaacc gcgcctgcaa
     4201 cgcactcgtg ccttccgcgc ccgcgcg
//
LOCUS       DAOPBQ010000325         4225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17213_length_4225_cov_3.758273, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000325 DAOPBQ010000000
VERSION     DAOPBQ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17213_length_4225_cov_3.758273"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2203)
                     /locus_tag="PKW60_10510"
     CDS             complement(<1..2203)
                     /locus_tag="PKW60_10510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPJ99907.1"
                     /translation="MIFVTVGAQMPFDRLIAALDHWAETSKRTDIFAQIGESSFRPQH
                     LRWTKFVNPDEFRELVETADVIVAHAGMGSVLTALELGKPIIVMPRRGALHETRNDHQ
                     VATARQLGNQGLATVAWDEYELLEKLDSQLEQVEAADKIPRNASNGLCNALMEFISRS
                     RSGIVNGVICFGGVDWWYHNRGHYDIQMMREFSRHTPVLYINSIGMRTPRLAEGKVFA
                     GRVLRKMKSWSHGLELVRENFAVMSPISIPKYRSSAAAKALLAEQVRDAAAWMGITRP
                     LVWVAVPTAAEVLDRIPSEGLVYQRTDRFEHYPDVNPERIRACDQRLKVEADLTLFCS
                     RSVFEEESAECRMAAFVDHGVDFEQFRSAGNGPVSEPGDVRAIPHPRVGFVGGIDAHT
                     FDPELFLQTAAALPECAFVMVGACSLPEGWCNLPNVHFLGRKAYDEVAAYMAACDVLI
                     MPWNRSPWIKACNPVKLKEYLAVGRPVVSTPFDELEHYQGLVCVADDAQKFAEGIRTA
                     LKAQPDADAMRQRVEQETWTAKARMVLENLQQAGVSFRGTPAARRRNGAAGQTSAGVL
                     SETRAPAHTNHKRQLLPLGLLLPSKRGSYWRKRHFAAAGMLALLAVWVTRDAWADILR
                     LASRNEECSHIWLVIPVALWLAKVRREEVSVPRRSSYLLAPLLVVLGWALYSLGDTFL
                     FQSLWHLGAVVIVVGCALSVLGGSVLLRFWPSFAVLVFLVPVPKIVQQQIGL"
     gene            complement(2200..2658)
                     /locus_tag="PKW60_10515"
     CDS             complement(2200..2658)
                     /locus_tag="PKW60_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HPJ99908.1"
                     /translation="MGVSRKKILAVSSGGGHWVQMMRLADVFRAHDVTFATVSKAYGC
                     DVPGAAVYAVVDATAWNKLKLLVQALEVLLILLRVRPDVVVSTGAAPGYFAIRLGKLL
                     GARTIWIDSIANVERLSMSGARVGEYADLWLTQWPHLALDSGPAYRGAVL"
     assembly_gap    2749..2758
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3189..3380)
                     /locus_tag="PKW60_10520"
     CDS             complement(3189..3380)
                     /locus_tag="PKW60_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99909.1"
                     /translation="MLSKQIFTAITARHSKGHDENTCGLHDNGVDPKPAHLEQKKPRK
                     VTLLSPAFAWTASRRESIK"
     gene            3482..>4225
                     /locus_tag="PKW60_10525"
     CDS             3482..>4225
                     /locus_tag="PKW60_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99910.1"
                     /translation="MSRSTRRSFLKHSVAGAVGAAAAPMILPSGVLASAGSPGANDRI
                     IIGGIGIGVMGRGHMRSFSSYDDVRIAAVADVDMRRANAAAEGLGADAYQDYRYVLDR
                     DDIDAVIIATPHHWHALNTIHAAQAGKDIYCEKCFSLTVREGRLMAEAVAKYNRVLQS
                     GMQQRSGEKEYKGCMLVRNGRIGKVSRVVTHHYLSPWLNGLPEEPVPPELDWDMWCGQ
                     VEPHPYNHRLTADSPDIPYGAWYSMREFGG"
BASE COUNT          865 a         1280 c         1234 g          836 t
ORIGIN      
        1 gaagcccgat ctgttgctgg acaattttgg ggacgggcac gagaaacacc agcacggcga
       61 acgagggcca gaatcgcaga agcacgcttc cgcccagcac ggaaagggcg catcccacca
      121 caatcaccac tgccccgaga tgccagaggc tctgaaaaag gaaggtgtcg ccaagagaat
      181 agagtgccca cccgagcacg accagaaggg gcgcgagcag gtagctcgac cgccggggaa
      241 cactcacttc ttcccgccgt accttcgcca gccacaatgc gaccggtatg accagccaga
      301 tgtggctgca ttcttcgttc cggctggcca gccggagaat gtccgcccac gcatcgcgag
      361 tgacccagac tgcgagaagc gccaacatgc ctgcggcggc aaagtgacgt tttctccaat
      421 aactccctcg tttcgaggga aggagcagtc ccagcggaag aagctgccgc ttgtggttgg
      481 tatgtgccgg cgccctggtt tcactcagca ctccagctga ggtttgcccg gcggcgccgt
      541 ttcttcttct ggctgccggg gtaccccgaa atgagacacc agcctgttgc agattctcca
      601 gcaccatcct cgccttcgct gtccaggttt cttgttccac acgctggcgc atggcgtctg
      661 cgtccggttg ggctttcagg gcggtacgaa tcccttcggc aaacttttgc gcatcatcgg
      721 cgacgcagac cagtccctga taatgttcca attcatcgaa cggcgtgctg acaaccggcc
      781 gccccacggc aagatattcc ttcaatttga ccggattgca ggcttttatc catggacttc
      841 ggttccacgg catgatcagc acgtcgcacg ccgccatgta ggctgcgacc tcgtcgtagg
      901 ctttgcgccc gaggaaatgc acgtttggga gattacacca tccttcaggc aatgagcagg
      961 cgcctaccat cacgaacgcg cattcaggca gcgccgcggc ggtttgcagg aacagttccg
     1021 gatcgaaggt gtgggcgtct atcccaccga caaaaccgac cctggggtgt ggaatcgcgc
     1081 ggacgtcgcc gggctcggat actggcccat tacccgccga ccggaattgc tcaaaatcca
     1141 ctccatgatc cacgaaggcg gccatccggc attccgcgga ctcctcctca aagacgcttc
     1201 tggagcagaa cagggtcagg tctgcctcaa ctttcagcct ctggtcgcag gcgcgaatgc
     1261 gctcgggatt cacatcggga tagtgctcaa aacggtccgt ccgctgatac accaggcctt
     1321 ccgaggggat gcgatcgagc acctccgccg cggtcggtac ggcgacccag accagcgggc
     1381 gcgtgatgcc catccaggcc gcggcatccc ggacctgctc cgcaagcaag gcttttgccg
     1441 ccgcgctgct gcggtacttc gggatggata tggggctcat cacggcaaaa ttttcgcgca
     1501 cgagctccag accatgactc cagctcttca tcttgcgaag gacgcggccc gcgaagacct
     1561 tcccttcggc aagccggggg gtgcgcatcc caatggagtt aatgtacaga acgggcgtat
     1621 gacggctgaa ctcccgcatc atctggatat cgtaatgacc gcggttgtga taccaccagt
     1681 ccacgccgcc aaaacagatg acgccgttga cgataccgct gcgcgatcgc gagatgaact
     1741 ccatcagcgc gttgcacagt ccgttcgacg cgttccgggg gattttgtcg gctgcttcga
     1801 cctgctccag ctgagagtcc agcttttcaa ggagttcata ctcatcccat gcgaccgtcg
     1861 ccagaccttg attgcccaat tggcgcgccg tcgccacctg atggtcattc cgcgtttcgt
     1921 gcagagctcc gcgccgcggc atgacaatga tgggtttgcc cagttccagc gccgtcaaca
     1981 cgcttcccat cccggcatgg gctacgatca cgtcggcggt ttcgaccagt tcgcgaaact
     2041 catcgggatt gacaaatttg gtccaacgga ggtgttgggg gcggaagctg ctctcgccga
     2101 tttgtgcaaa gatgtccgtg cgtttgctcg tttccgccca atgatccaac gcagcgatga
     2161 gtcgatcaaa cggcatctgt gcgccgaccg tgacgaaaat cataggaccg ccccccggta
     2221 cgcagggccg gaatcgaggg ccagatgagg ccattgagtc agccagaggt ccgcgtattc
     2281 gccgacccgg gctccggaca tggacaggcg ttcgacgttc gcaatgctgt caatccagat
     2341 agttcgcgcc cccagcagtt tacccagacg gatggcgaaa tatccgggtg cagcccccgt
     2401 ggagacaaca acgtccggcc ggacccgcaa gaggattagc agcacctcga gggcctgcac
     2461 aagcagcttc aacttgttcc aggcggtcgc atccactacc gcatacaccg cggcgccggg
     2521 aacatcgcac ccataggctt tgctgacggt ggcaaaagtc acgtcatgcg cgcggaagac
     2581 gtccgccagc cgcatcatct gcacccagtg gcccccgccg gacgataccg caaggatctt
     2641 cttgcgagac acgcccattt ccggcgtagc tgcggggatg gtctgcccgc gcggcgcccc
     2701 gtgactctcc ggtacgggcc gggtggcgtg gttgcacatc ccgattccnn nnnnnnnnct
     2761 ccgcactcca cgtttctgtg cgcccatttg ctcccgaaag atgatcggct ttcactccgc
     2821 ccgtccccgc ctggtgtgcg ccagtctgcc gttcgcctgc tttgcatcag caagtagatg
     2881 ccagtaacag catacagcta gattcaatac ggcaactaga ttagctccac aaaagtatgt
     2941 acattctcct gttaaaatag caattcaagt gccaaaaccg caagtatttt ctaaaacggg
     3001 actacacaaa cagttgcgtg gcacgtagcg ccccaaaatg tccgagtttc gctaaccggg
     3061 agacacattt ttgtcagccg tgacaaacta tatgtcattt cttgcctcat caggaggctc
     3121 gtggcattca tacccttttg agcgctgtaa aaaccgtgac aaaatgccat gttgtccact
     3181 gattcatact atttgatgct ctcccggcgt gaagccgtcc aggcgaaagc gggagagagg
     3241 agggtgactt ttcgcggctt tttttgttcc agatgggcgg gtttggggtc cactccatta
     3301 tcatgcaagc cgcaggtgtt ttcgtcgtgc cccttggaat gccgggcggt gatggccgta
     3361 aagatctgct ttgaaagcac ggagcaaggt ctctatgata agggttgcat tgcccgggaa
     3421 caggacaagc tggtttcggg gaaaaggcgt ggtctgagat gaacacgagg gagaggatcc
     3481 gatgagtcgt tcaacgagac gcagtttctt gaagcacagc gtggccggtg ctgtgggcgc
     3541 ggcagccgcg ccgatgattc ttccttcggg cgtgctggcc agcgcgggga gcccgggcgc
     3601 taatgaccgt atcatcatcg gtggaattgg catcggcgtg atgggcaggg ggcacatgcg
     3661 ctccttttcc agttatgacg acgtgcgcat cgcggcggtg gcggatgtcg atatgcggag
     3721 agccaatgcg gccgccgagg ggttgggcgc ggacgcctac caggattacc gctacgtgct
     3781 ggatcgcgac gatatcgacg cggtcatcat cgcaacgccc caccactggc atgccctgaa
     3841 cacgattcat gcggcccagg cgggcaagga catctattgc gagaagtgct tctcgctgac
     3901 cgtccgcgaa gggcgcctga tggcggaggc cgtggcgaag tataaccggg tcttgcagtc
     3961 cggtatgcag cagcggtcgg gagagaagga atacaaaggc tgcatgctcg tacgcaacgg
     4021 ccggatcggc aaagtcagcc gcgtggtgac ccatcattat ctgagcccat ggctgaacgg
     4081 tttgcccgaa gagccggtcc cgcccgaact tgactgggat atgtggtgcg gccaggtgga
     4141 gccgcatccc tacaatcacc gcctcaccgc cgacagcccc gacatcccgt atggcgcatg
     4201 gtattccatg cgtgaatttg gcggc
//
LOCUS       DAOPBQ010000326         4220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17240_length_4220_cov_3.316447, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000326 DAOPBQ010000000
VERSION     DAOPBQ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17240_length_4220_cov_3.316447"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1780)
                     /locus_tag="PKW60_10530"
     CDS             complement(230..1780)
                     /locus_tag="PKW60_10530"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HPJ99911.1"
                     /translation="MKNTDWRASCCAVLLSMLLVLTAASCGPKTAPLPATPPPVTAPE
                     ASAEAPAAPLAPAPEISAAAPAAAPTFTSPFPIPEEKKTALDRYVETPDPAFAYDTPP
                     AKVDENDSYTARTYHMVSQTWLKPEQVDRTKWEHWLIIIEPKEVAQPEALMFIGGGSN
                     KPDAEPPRADSGLVRVATMTKSVVAEIKQIPNQRLKFPDEHDDRYKEQGRTEDEIISY
                     CWDKFLVTGDAVWLTRLPMTKAVMRAMDVVQKELPSVEKFTVAGGSKRGWTTWTVGAA
                     DPRVVAIMPAVIDVLNVVPSLDNHYAAYGFWAPAISEYEDMNVLARLHTPEFKALCGI
                     VDPYSYIDRLVMPKYIINATGDQFFLPDSWRFYFDDLKGEKYLRYVPNTDHGLSPEAY
                     LNLASFHNAIINDTPRPEFTWRMEDDGNFVVECTTQPTKVLLWQASNPDARDFRLQTI
                     GQAWQSTELAPAEPGVYKTKMAAPEKGWTGFMVELEFPNPGFPLPFKFTTGVSVLPQV
                     WPFTAPGT"
     gene            complement(2305..2760)
                     /locus_tag="PKW60_10535"
     CDS             complement(2305..2760)
                     /locus_tag="PKW60_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HPJ99912.1"
                     /translation="MAQNNRALYEQVILEHNRKPRNYRAMEDADRRIEGHNPLCGDHF
                     IIYLKLDGDVISDLSFEGQGCAIAKSSASLMTSLLKGKSIREAEDLFVQFHRLVTASP
                     DAPVDEKAVGKLAVFAGVREFPIRVKCATLSWHALLSAVRGMHSTATTE"
     gene            complement(2760..4010)
                     /locus_tag="PKW60_10540"
     CDS             complement(2760..4010)
                     /locus_tag="PKW60_10540"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPJ99913.1"
                     /translation="MISELPADAAPVLDVAALRRDFPILQTKVHGKPLVYLDNAATSQ
                     KPQAVLDALSRYYAESNANIHRGIYQLSEDATRAYAAARLRVKNFIHARLKCEIVFTR
                     GTTESINLVAQTYGRQRVKAGDEILITHMEHHSNIVPWQVLCEEKGARLRVVPITDTG
                     ELDVEAFQRMLTGRTKLAAITYVSNALGTVNPIKELVAMAHAQGVPVLVDGAQAVPHL
                     PINVLDLGCDFFVFSGHKMFGPTGIGVLYGRAELLEEMPPYQTGGDMILSVRFDKTVF
                     NSLPHKFEAGTPDIAGAIGLGAAIDYLNRVDLARVAAYEAELLEYGTEVLRAVDGVRI
                     VGNAAHKASVLSFVMNAAHPHDIGQMLDGEGIAVRAGHHCAQPLMERLGVPATARASL
                     AFYNTREELDALGRALHKINEVFG"
     gene            complement(4054..4209)
                     /locus_tag="PKW60_10545"
     CDS             complement(4054..4209)
                     /locus_tag="PKW60_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99914.1"
                     /translation="MFYFQSRGISEAMARAILTCGFAGEVVDDISVEPLRTRLHDYIY
                     NRYSPLK"
BASE COUNT          779 a         1250 c         1310 g          881 t
ORIGIN      
        1 ccggcgacta tgcgccgatg agcctcgacg atatgaaaaa ggtctacggc aagtagagct
       61 cgataacacg gtattgagtc cacggctgcg ccgcccttcc ctggggcggc gcagcgcctt
      121 cttggggggg aggtcagcga aaggcgcccc gcataacagc gccgcgctcc ggaatcgctg
      181 tggatttcgg agcgcggcgg aaaagcgttc cagcagtatc gcggcgtgtt caggtccctg
      241 gagcggtgaa gggccacact tgcgggagca cgctcacgcc ggtcgtgaat ttgaagggca
      301 gcggaaagcc cgggttcggg aactcgagct cgaccatgaa gcccgtccat cctttttccg
      361 gcgcggccat cttggttttg tacacaccgg gctccgcggg ggccagttcc gtgctctgcc
      421 aggcctggcc gatggtctgg agacggaaat cgcgcgcatc cggattgctg gcttgccaca
      481 gcaggacttt tgtgggctga gtcgtgcatt ccacgacaaa attcccgtcg tcttccatgc
      541 gccaggtgaa ttcaggccgc ggcgtatcat tgatgatcgc attgtggaac gacgcgaggt
      601 tcaaatacgc ttccggactg aggccgtggt cggtgttggg gacatacctc aagtatttct
      661 cgcctttgag gtcgtcgaaa tagaaacgcc acgaatccgg caggaaaaac tggtcgcctg
      721 tcgcattgat aatgtatttg ggcatcacga gccggtcgat gtacgaatac gggtccacga
      781 tgccgcacaa ggctttgaac tccggcgtgt gcaaccgcgc cagcacgttc atgtcctcgt
      841 attcgctgat ggcgggcgcc cagaatccgt aggcggcgta atgattgtcg agcgatggca
      901 ccacgttcag cacgtcaatc accgcgggca tgatcgccac cacccgcgga tcggcggcgc
      961 cgactgtcca ggttgtccag cctcgtttgg agccgccggc caccgtaaat ttctccaccg
     1021 aaggcagttc tttctgcacc acatccatgg cgcgcatgac cgccttggtc atcggcaagc
     1081 gcgtcagcca aacggcatcc cccgtcacaa ggaacttgtc ccagcaatag gagatgatct
     1141 catcttcggt gcggccttgc tccttgtacc ggtcgtcgtg ttcatccggg aatttcaggc
     1201 gctggttggg gatttgtttg atttcggcca ccacggattt ggtcatcgtg gcgacacgca
     1261 ccagccccga atccgcgcgc ggcggctcgg catcgggttt gttgctgccg ccgccaataa
     1321 acatcagcgc ctcgggctgc gccacttctt tcggctcgat aatgatcagc caatgctccc
     1381 acttcgtgcg atcgacttgt tccggcttca gccaggtctg cgataccatg tggtaggtgc
     1441 gcgccgtgta gctgtcgttc tcatcgacct tcgcgggcgg tgtgtcgtag gcaaaggccg
     1501 gatcgggcgt ctcgacatac cggtcaagtg cggtcttttt ctcctccgga attgggaagg
     1561 gcgatgtaaa cgtgggcgct gctgctgggg ctgctgcgct gatttccggg gccggcgcga
     1621 gcggcgccgc tggggcttcg gcgcttgctt ccggcgccgt cacgggcggt ggcgtcgcgg
     1681 gcagtggtgc ggtcttcggg ccgcacgacg ctgccgtaag aacgagcagc atcgacaaca
     1741 aaaccgcaca acaggacgct ctccaatcag tgtttttcat gagtttcacc tttccctacc
     1801 gcattttgta cacttttcag attttctcga cgccattatg gtaaccgatg tacatcggtc
     1861 gatcaacttc tgtttgcggc cttccaatgt cccggccaaa cgcaaaaaat tgcctcttgc
     1921 tgccgccctg ccagccaagg gcagccgtgc gcaccggagc gggcgtttgc acggggctcc
     1981 tccgtccacc gatggcccag tcaggcgcgg acgttgagaa tcaacccgcg agcggagact
     2041 caaagccacc cggacacgtg cattcacggg gttcccatcg gagaatgagc aattccatcg
     2101 cccacggcgc tgcggggcgg gttggcctgt tctcaacctc acggccaacc ttccatgcgc
     2161 gacaagaaac ccgatagtat gatgatacac gccccggtaa gattagcaaa tagcttggca
     2221 ggccgctact cacatggcgg atgggacgga cctgggcgat ttgggaaacc caggagatca
     2281 ggtacggtat ctctgccttg atggctattc cgttgtggcg gtcgaatgca tcccgcgcac
     2341 ggcgctgagc aaggcatgcc aggacaaggt ggcgcatttg acacggattg gaaactcccg
     2401 gacccctgca aagaccgcta gtttgccgac ggctttctca tcgacgggtg catccgggct
     2461 ggccgtgacc aggcggtgga actggacgaa aagatcctcg gcttcgcgaa tactcttgcc
     2521 tttgaggagg cttgtcatga gtgaggccga tgatttcgcg atggcgcaac cctggccctc
     2581 aaagctgagg tctgagatga cgtcgccgtc cagtttgaga tagatgatga aatggtcgcc
     2641 gcataacggg ttgtgccctt cgatgcggcg gtccgcgtcc tccatggcgc gataattccg
     2701 cggtttgcgg ttgtgttcca gaatcacctg ttcgtacagc gcgcggttgt tctgtgccat
     2761 cagccgaaaa cctcattgat cttgtggagg gccctgccca gggcgtcgag ctcttcgcgc
     2821 gtattataga acgccagcga ggcgcgcgcg gtagccggca cgccaagacg ttccatcagg
     2881 ggttgtgcgc aatggtgccc ggcgcgcacg gcgatgccct cgccgtcgag catctggccg
     2941 atatcatgcg gatgggccgc attcataacg aaggacaaaa cggaggcttt gtgtgcggcg
     3001 tttccgacaa tgcgcacgcc gtcaacagcg cgcaggacct cggtcccgta ttcaagcagt
     3061 tcggcctcat aggctgcaac tcgggccagg tccacgcgat tgaggtagtc gatagcagcg
     3121 cccaggccaa ttgcgcccgc gatgtcgggg gtgccggcct caaacttgtg cggcagtgaa
     3181 ttgaagaccg ttttgtcgaa ccggacggac agaatcatgt cgcctccggt ctgataggga
     3241 ggcatctcct cgagcagctc ggccctgccg tagagcacgc cgatgcccgt ggggccgaac
     3301 attttgtggc ccgagaacac aaagaaatcg caacccaggt ccaggacgtt gatcggcagg
     3361 tgagggactg cctgcgcccc atcgaccagc acaggcacgc cctgggcatg ggccatggcg
     3421 accaattcct tgatgggatt cacggttccg agcgcattcg acacgtacgt gatcgccgcc
     3481 agttttgtac ggccggtcag catgcgctgg aacgcctcga cgtcgagctc gcccgtgtct
     3541 gtgatgggga caacgcgcag ccgggcccct ttctcctcgc acagcacctg ccagggcaca
     3601 atgttggaat gatgctccat gtgtgtgatg agaatctcgt caccggcctt cacgcgctgg
     3661 cggccgtagg tctgggcgac caggttgatg ctttccgtcg tgccccgcgt gaagacgatc
     3721 tcgcatttca gccgggcatg aataaagttt ttcacacgga ggcgggccgc ggcgtaggcc
     3781 cgggtcgcgt cttcgctgag ctggtagatc ccccggtgga tgttcgcgtt gctctcggca
     3841 taatagcggg acagcgcgtc gagcacggcc tgcggtttct ggctcgtggc cgcgttatcg
     3901 aggtacacga gcggtttccc gtgtactttt gtctgcaata tggggaagtc ccgccggagc
     3961 gccgcaacat cgagcacggg cgcggcatcg gctgggagct cagatatcat ttcccggaca
     4021 gctcgcatgg tgtccgcctt tctgtgttca ttctcatttc agggggctgt agcgattgta
     4081 gatgtagtcg tgcagccggg tccgcagcgg ctccacggag atgtcgtcga cgacttcacc
     4141 cgcgaacccg caggtcaaga tggcacgggc catcgcttcc gagatgcccc ggctttggaa
     4201 atagaagatc tgttcccggg
//
LOCUS       DAOPBQ010000327         4213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17287_length_4213_cov_3.762626, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000327 DAOPBQ010000000
VERSION     DAOPBQ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17287_length_4213_cov_3.762626"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PKW60_10550"
     CDS             <1..883
                     /locus_tag="PKW60_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99915.1"
                     /translation="MRAMRTMTVALAVALMAAPVAAEAITGELHDRAVATLRSALHTE
                     TAWVRVHAAEALLRNGLPEGVEAVFLPEVETSPAQHRIGVWRVLARAQQNPERREHYV
                     ARIRTAFLDLDGPDRLHAAETLGKLGDARRSTALLLAAVENDTPMSAYARLILANAGS
                     VEDTDYLAALLGSYDEKIRETVGYALRFAPNMAESTLARVREAALTEPEDSSARLYLL
                     IAWLAHASDADRGAIMKLLKPYAESSVVSARYQFGEAMVLCGGAEDVPVLRRLLDDEN
                     VDVQVSAANALLAVERR"
     gene            complement(969..3668)
                     /locus_tag="PKW60_10555"
     CDS             complement(969..3668)
                     /locus_tag="PKW60_10555"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPJ99916.1"
                     /translation="MKVNVSASCAVLLMALLAIACTPATEAPPAPTAPASAPEPAPAA
                     VAEPPAPESEAAPSASVIPDVPLPEVPGLATEGLVLAQGGSSPYVIVVEPTASPSEKL
                     AASEFQTYVKACTDAELPIVEGLPAEEAPMIVLGCGPVAQGLGVDPSAEELGDQGYVL
                     VTVGPSVVIAGSAAAGTLYGARDFLEADLGVRWYAPDETRTPHLETAFVRERADTQVH
                     RPSFKWRLTSYTWPGGDAAFRARRGENNGTGGPDNELGIQYAHDGRCHTYFRFISPAE
                     YFDEHPEYFSEIGGKRVRDETQLCLTNPDVLDIVTEKMLQRMRANPQYEQQNFSQEDW
                     YSYCECANCTAMNERLGTTGGTQFWFVNQLAERTSREFPDIQIGTLAYIYTEEPPREL
                     KMHPNVAVWLCHMFPSCQSHPIATCIKNADFKRRALKWAEICDHLYIWYYIVDFAHYY
                     NPYPNFGAMAADYRFFHDIGVEGIYSQAMGHRGGGGEFSLLRPYYATELMRNHALDPD
                     VVMYDFLQGYYGDAAPPIYRYIKMLQHKVDADDIHMHLYTNPGQGYLPDAIVAAGQEL
                     FDQAEEAVANDEKLLDRVRVCRMPLMYARWFPRNGYEVVDGKMLFKGDLAKPGEMMAF
                     AERMAAHGFINIREWAGSPEELALYSAALNTPIPLPTITNAHLAVEVAPFIGGRALRI
                     IDKATGECVTAYSVVPSLVFPFCGGEDTRVGVNFSVHEGGPMALAEVTEQSENNDAIT
                     LSLEARGFKIERHIALVPDRPAVVFTITVTNPGDKAVSTRVKSHLELDLGDLATTKMA
                     FQSMSGESIAMDMHPVIEQMREGRMFHADTQTLPDGEWAFSGSKGLKVIQRFDPQTVD
                     LAWVYAFPEELQDLEVELWRIEEEIPPGQSVTLRHELEIQPAP"
     gene            complement(3833..>4213)
                     /locus_tag="PKW60_10560"
     CDS             complement(3833..>4213)
                     /locus_tag="PKW60_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HPJ99917.1"
                     /translation="MEALNAHGKPLKGARVLILGAAYKKDVDDMRESPALKLMELLLE
                     HGAEIAYNDPHIPQLPATRKYQFEMSSTPLTAETLERVDCVLVATDHSVYDYEFIVRH
                     APLVVDTRNATRAVATGREKIVKA"
BASE COUNT          766 a         1248 c         1386 g          813 t
ORIGIN      
        1 catgagagca atgcgaacaa tgaccgtggc gctggccgtg gcgctgatgg cggcgccggt
       61 cgcggcggag gcgataacgg gggagcttca tgaccgggcc gtcgcgacgc tccggagcgc
      121 gctgcatacc gagacggcct gggtgcgagt gcatgcggcg gaggcgcttt tgcgaaacgg
      181 attgccggaa ggcgtggagg ctgtgttttt gcccgaggtg gaaaccagcc cggctcaaca
      241 ccgcatcggc gtctggcggg tgctggcgcg cgcacagcag aacccggagc ggcgggagca
      301 ctatgttgcg cgcattcgga ccgcgtttct ggatctggat gggccggatc ggctgcacgc
      361 cgcggaaacc ctgggcaaac tcggcgatgc gcggcgctcg accgcccttc tcctggccgc
      421 ggtagagaat gacacgccga tgagcgctta tgcgcgcctg atcctcgcca acgcgggctc
      481 ggtcgaggat accgactatc tggccgcact gctgggttcc tacgatgaga agatacgcga
      541 aacggtcggc tatgcgcttc gttttgcccc gaacatggct gaatccacgc ttgcccgcgt
      601 gcgggaggct gcgctgacgg aacccgaaga ctcatctgca cggctctatc tgcttatcgc
      661 atggctggcc catgcgagcg acgcggatcg gggcgccatc atgaaattgc tgaaaccgta
      721 cgccgaaagc agcgtcgtat ccgctcgcta ccagttcgga gaggccatgg tgctctgcgg
      781 tggcgcggag gatgtccccg tgttgcgccg cctgctggac gacgaaaatg tcgacgtaca
      841 ggtcagtgcg gccaatgctc tgctcgctgt ggaacggaga tgagcgggcc ggcttcgcat
      901 agaaaaagaa ccgtccggag gaggacatcc tccggacggc aggtttttcg gacaggtggt
      961 cgcagccgct acggcgccgg ttgaatttcg agctcatgcc ggagcgtgac gctctggcca
     1021 ggcggaattt cttcttcgat gcgccacaac tcaacttcga gatcctgcag ctcctccgga
     1081 aatgcgtaga cccacgcgag atccacggtc tgcggatcaa agcgctggat caccttcagt
     1141 cccttcgagc ccgagaacgc ccactcgccg tcggggagcg tttgggtgtc ggcgtgaaac
     1201 atccggcctt cgcgcatctg ctcgataacc ggatgcatgt ccatggcgat ggattcaccg
     1261 ctcatgctct gaaaggccat cttcgtggtt gccagatcgc ccagatcgag ctcgagatgg
     1321 cttttcactc gcgtcgacac ggctttgtcg ccggggttgg tgaccgtgat ggtgaaaacc
     1381 acggccggcc ggtccgggac cagcgcgatg tggcgctcaa ttttgaagcc gcgcgcctcc
     1441 agggacagcg tgatggcgtc gttgttctcc gactgttcgg tcacttcggc cagcgccatc
     1501 gggccgccct catgcacaga gaagttgacg ccgacgcgcg tgtcttcgcc gccgcagaag
     1561 ggaaacacga ggctgggcac cacgctgtag gctgtgacgc attcgccggt ggccttatca
     1621 atgatgcgca acgcccgccc gccaatgaat ggcgccacct cgaccgcgag gtgcgcgttc
     1681 gtgatcgtgg gaagcggaat tggcgtgttg agggccgccg agtagagcgc gagctcttcc
     1741 ggcgaaccgg cccattcgcg gatgttgatg aagccgtggg cggccatacg ctctgcaaac
     1801 gccatcatct ccccgggttt tgcgaggtcg cccttaaaca gcatcttgcc gtcgaccacc
     1861 tcgtagccgt tgcgggggaa ccagcgggcg tacatcagcg gcatgcggca cacacgcact
     1921 cggtcgagga gtttttcatc gttcgccacg gcttcttcgg cctgatcgaa gagttcctga
     1981 ccggcggcca cgatcgcatc ggggaggtag ccctggccgg ggttggtgta gaggtgcatg
     2041 tggatgtcgt ccgcgtcgac cttgtgctgg agcatcttga tgtagcggta gataggcggc
     2101 gcggcatccc cgtaataccc ctggaggaaa tcgtacatga ccacatcggg atcgagggcg
     2161 tgattccgca tcagctcggt ggcgtaatac ggccggagca ggctgaactc gccgccgccg
     2221 ccccggtggc ccatggcctg cgagtagatg ccttcgacgc cgatgtcgtg gaaaaaccgg
     2281 taatccgcgg ccatggctcc gaaattgggg taggggttgt aatagtgggc gaaatcgacg
     2341 atgtagtacc agatatacag atgatcgcag atttcggccc atttgagggc gcggcgcttg
     2401 aagtccgcat tctttatgca cgtcgcaatg ggatggctct ggcagcttgg gaacatgtgg
     2461 cagagccaca ccgccacgtt gggatgcatc ttgagctccc ggggaggttc ctcggtatag
     2521 atgtaggcga gggtgccaat ttgaatgtca gggaactctc tggacgtacg ctcggccaac
     2581 tggttcacaa accagaactg cgtgcccccg gtggtgccca ggcgttcgtt catggccgtg
     2641 cagttcgcgc attcgcagta ggagtaccag tcttcctgag aaaagttctg ctgttcgtac
     2701 tgcggattgg cgcgcatccg ctgcagcatc ttttcggtca cgatgtcgag cacatcggga
     2761 ttggtcaggc acaattgggt ttcatcgcgc acacgtttgc cgccgatctc cgaaaaatat
     2821 tcgggatgct cgtcaaaata ctcagcaggg ctgatgaaac ggaaataggt atggcagcgg
     2881 ccgtcgtgcg catattgaat accgagttcg ttgtcggggc cgcccgtgcc gttgttttcc
     2941 ccgcggcgcg cacggaaggc tgcatctcct ccgggccacg tgtaactcgt gagccgccat
     3001 ttgaaggaag gccgatgcac ctgcgtgtcg gcacgttccc ggacaaacgc agtctccagg
     3061 tgaggtgtgc gggtttcgtc cggggcgtac caccgcacgc ccaggtcggc ttccagaaaa
     3121 tcccgcgcgc catacagggt acctgccgcc gccgacccgg cgatcaccac gctgggaccc
     3181 acggtgacca gcacatatcc ctgatcaccc agctcctccg cagaggggtc cacgcccagc
     3241 ccttgcgcca cgggaccgca gcccaggaca atcatcgggg cctcttccgc cggcagacct
     3301 tccacgatag gcaactcagc gtccgtgcag gccttgacat aggtctggaa ctcggaggca
     3361 gcgagctttt cggacgggct ggcggttggt tcgaccacaa tcacgtacgg actggagcca
     3421 ccctgtgcca gcaccaggcc ctccgtggcc aggccgggga cttccggcag agggacatcg
     3481 ggaattacgc tcgccgacgg ggctgcctca gattcgggcg ctggcggttc cgcgaccgcc
     3541 gccggtgcgg gttccggagc ggatgccggg gctgtggggg caggcggagc ctctgttgca
     3601 ggggtacatg cgatcgcgag cagtgccata agcagtacgg cgcaggatgc agatacgttc
     3661 accttcatgg caggcgccct ccggtaggtt cagcacggta tcgggcttca tcatggcaat
     3721 caatttgacg aaaagtcaat ttcaagcggc cgcatagtgg aacgccccac cccgcaacgg
     3781 ctgactggcc ggcaaggcag ggttaaaaac catgacccat ggtcgaattc ggctaggctt
     3841 tgacgatttt ctcgcggcct gtggccaccg cccgggtcgc gttgcgcgtg tcgacgacga
     3901 gaggggcgtg gcgcacgatg aactcgtagt cgtagacgct atggtcggtg gccacgagga
     3961 cacagtccac gcgttcaagc gtttccgcgg tgagcggcgt ggaactcatc tcgaactggt
     4021 acttgcgcgt ggcgggaagt tgcgggatgt gagggtcgtt gtaggcgatc tccgcgccat
     4081 gctcgagcag cagctccatg agcttcaacg cgggggattc acgcatgtcg tcgacgtctt
     4141 ttttgtatgc ggcgcccagg atgagcacac gcgcgccttt gaggggcttg ccgtgggcgt
     4201 tgagcgcttc cat
//
LOCUS       DAOPBQ010000328         4207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17323_length_4207_cov_4.020231, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000328 DAOPBQ010000000
VERSION     DAOPBQ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17323_length_4207_cov_4.020231"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="PKW60_10565"
     CDS             <1..1722
                     /locus_tag="PKW60_10565"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HPJ99918.1"
                     /translation="WEYPGMLSLQCLAYCQDNGPGLYISTDDADAFAKRFCAFGGAEN
                     RAGVEVVHLPQAASDGANRYEPEYRVWLGAFVGDWFTAAERYRVWALQQPWAQHARLK
                     TGQVEDWATETALWVWNRGRSNQVLTPARALQERTGLPVSVFWHWWHGCPYDIGFPEY
                     FPPREGAEPFREAVKSAKDAGVNAMVYMNQRLWGMTTESWVAEGAERFAVKGPDGQVR
                     PEIYNTFQPAPCAAMCMGTAFWRNTYAGLAEQAINGLGVSAIYMDQACTSLPCYDPAH
                     GHPIGGGTYWMEGFRSLQDDIRNRCARPSDAGGQTNAVLAGEGCGEAWLPYLDLMLSL
                     QVSIERYAQPGIWEPIPFFHAVYHGYAIFFGNYSSLTMPPYDDLWPAEFAPKNPLELL
                     DTKFSTQFRLEQARAFVWGQQPTIANFLPAMFEERAAELTYLVRLAKVRQQGLDYLLH
                     GTMLRPPAIALTEEEIDISRLSIYAGQQDALKQYRKAIPRVLASAWQAGDGRVAIVLA
                     NLTGEPQQFPLELPRDAYPLPERSELRRIDESEVREMGRFNNDGPLAVDLPPFGVCLY
                     TFTAAAA"
     gene            complement(1749..2633)
                     /locus_tag="PKW60_10570"
     CDS             complement(1749..2633)
                     /locus_tag="PKW60_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPJ99919.1"
                     /translation="MRFVQLSAVFAGLALLAGSPEALDVEAPFGWHAGPTTWTFNRFT
                     LFEAIEKTASVELKYVEAFEGQRVRPDADLKLNAGLPDEAIEAIRAKLDECGVEMPGM
                     YIHTIPGEEAACRRTFEFARKLGLRYIVSEPEPETLDVIERHCDEYGINVAIHNHPEG
                     KSWYWSPEEALKVCEGRGPRIGVCADTGHWLRSGLDPAESIRKLGKRLLTLHLKDLDR
                     AAPDGTDRPWGQGCGDLEQVLRAIRELELTPALFGIEYETNWENNLPDVVECAAWLKK
                     TVAALAAEEAQQKEPAQE"
     gene            complement(2636..3898)
                     /locus_tag="PKW60_10575"
     CDS             complement(2636..3898)
                     /locus_tag="PKW60_10575"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99920.1"
                     /translation="MAAGAAAAAPTILPASVWGAEDAPPPSETVSVAFIGVGGMGRHN
                     LKDFLAQKACRVVAVCDVYRDRRESTKAVIDEANGDQGCAMYGDFREVLAREDIDAVS
                     IAVQDHWHAVMAVAAAKAGKDIYCEKPLGISFEESQLIRNTVRATGRVFQTGTWQRSL
                     EHFQRGCTLARNGYLGKIHTLEVAAPGPVYVPKYDGPLDPQPVPDGFDWAMWRGPAPD
                     KPYNPGRVAWPDWYLIWDYCVGFICNWGVHNLDQANWGCPEVGTQPFELQCRADYRKQ
                     GFTDNVNGWDATFTYPGGLTMIFKDSDKMLQGCRFIGDEGWVHVSRQGITAEPESLLT
                     VEFKESDARLTNSTNHAADFLDCMKTRNDPVSSVDSAHTATSLGILADIAARLEQNLS
                     WDPAAERFVDNDAANAMLHRDMHNGWEV"
BASE COUNT          749 a         1360 c         1279 g          819 t
ORIGIN      
        1 tgggaatacc cggggatgct ctcgctgcag tgcctcgcct actgccagga caacggtccg
       61 ggcctctaca tcagcacgga tgatgcggac gctttcgcga aacgcttctg cgcgtttgga
      121 ggcgccgaaa accgggccgg cgtggaggtg gtgcaccttc cacaggcggc ctcggatgga
      181 gcaaaccgct acgaaccaga gtaccgggtg tggcttgggg cttttgtggg cgattggttc
      241 acggctgccg aacgataccg cgtgtgggcc ctgcagcagc cctgggctca gcacgcacgc
      301 ctgaaaaccg ggcaggtgga agattgggca accgaaaccg cgctctgggt gtggaatcgc
      361 ggccgctcca accaggtgct caccccggct cgtgcccttc aggagcgcac gggcctgccc
      421 gtgagcgtct tctggcactg gtggcacggc tgcccctacg acattggatt cccggaatat
      481 tttccgccgc gggaaggcgc ggagcccttt cgcgaggccg tgaaaagcgc gaaagacgcg
      541 ggggtcaacg cgatggtcta catgaaccag cgtctgtggg ggatgaccac ggagagctgg
      601 gtggcggaag gcgccgaacg tttcgcggtg aaagggcccg acggccaggt gcgccctgaa
      661 atctacaata ccttccagcc cgcaccctgc gccgccatgt gcatgggcac cgccttctgg
      721 cgcaacacct acgccggact cgcggaacag gcaataaatg gcctcggcgt cagcgccatc
      781 tacatggatc aggcctgcac gagcctgccc tgctacgacc cggcacacgg ccatcccata
      841 ggcgggggca cctactggat ggagggcttc cgcagcctgc aggacgatat ccgtaaccgg
      901 tgcgcaaggc catctgatgc cggtggccag acaaatgccg ttcttgccgg tgaaggctgc
      961 ggcgaggcct ggctgcccta tctcgatctg atgctcagcc ttcaggtgag catcgagcgc
     1021 tacgcccagc caggcatctg ggaacccatc cccttcttcc atgcggttta tcacggttac
     1081 gccatttttt tcgggaatta ctcatcgctg accatgccgc cttacgacga cttgtggccc
     1141 gctgaatttg cgccgaagaa tcctctcgaa ttgctcgaca cgaaattctc gacgcagttc
     1201 cggctggaac aggcccgtgc gtttgtctgg ggacaacagc ccacgatcgc caactttctc
     1261 cccgccatgt ttgaagagcg tgcggcggaa ctgacgtacc tggtccgcct ggcgaaggtc
     1321 cgccagcagg ggttggatta tcttctccat ggaacgatgc tgcggccgcc cgccatcgcg
     1381 ctcacggagg aggaaattga tatctcgcgg ctgtccatct atgcggggca gcaagacgca
     1441 ttgaagcaat accgaaaagc gattccgagg gttctggcca gcgcatggca ggctggcgac
     1501 gggcgtgtgg ccatcgttct cgcgaatctg accggtgagc ctcagcagtt cccgctcgaa
     1561 ctgccccgtg acgcgtaccc cctccccgag cggagcgagc tgcggcgcat cgatgagagc
     1621 gaggttcggg agatggggcg gttcaacaac gacgggcctc tcgcggtgga tctaccgcct
     1681 ttcggcgtgt gcctgtacac gtttaccgct gccgcggcat gaattgagcg cccggcgtga
     1741 ccgctgggct attcctgtgc cggttccttc tgctgcgctt cctcggcggc cagggcggcg
     1801 acggtcttct tgagccacgc cgcgcattcc acgacatccg gcaggttgtt ctcccagttt
     1861 gtctcgtatt cgatgccaaa cagggccggt gtgagctcaa gttcacggat agcccgcagc
     1921 acctgttcaa ggtcaccgca accctggccc catggccgat ccgtaccatc cggcgcggct
     1981 cggtccaggt ccttcaagtg cagggtcagc aggcgtttgc cgagtttgcg gatggattcg
     2041 gccgggtcca gtcccgagcg cagccaatgg ccggtgtcag cgcagacgcc gatgcgtggg
     2101 ccacggccct cacagacctt cagcgcctcc tccgggctcc agtaccacga tttgccttcc
     2161 gggtggttgt ggatcgccac attgatgccg tattcgtcgc aatggcgttc gatgacatcc
     2221 agggtctcgg gctcgggttc cgaaacgatg taccgcaggc ccagtttacg ggcgaactca
     2281 aacgtgcgcc ggcacgctgc ttcctctccg gggatcgtat ggatgtacat ccccggcatt
     2341 tcgactccgc attcgtcgag tttggcgcgg atcgcctcaa tcgcctcgtc aggcaatccc
     2401 gcgttcagct tcagatcggc atcgggacga acccgctgcc cctcgaaggc ttcaacgtat
     2461 ttgagttcaa cggatgcggt cttctcgatg gcttcaaaga gcgtaaaccg gttgaacgtc
     2521 cacgtggtcg ggccggcatg ccagccgaat ggcgcctcca catccaaggc ctccggactt
     2581 cccgccagca gcgccaggcc ggcgaacacg gcggacagtt gaacaaagcg catggctaca
     2641 cctcccaccc attatgcatg tcgcggtgca acatggcatt tgcggcgtcg ttgtccacaa
     2701 atcgttccgc agccggatcc caggacaggt tctgttcgag acgcgccgca atgtccgcca
     2761 ggatgcccag ggacgtcgcg gtatgcgcag aatcgacgct ggatacggga tcgttgcggg
     2821 ttttcatgca gtccagaaaa tcggccgcgt ggttcgtgga gtttgtcagg cgcgcgtcgc
     2881 tctccttaaa ctccaccgtc aacagcgatt cgggctccgc cgtaatcccc tggcgggaca
     2941 catgcaccca tccctcatcg ccgataaagc ggcatccctg cagcatcttg tcgctgtctt
     3001 tgaagatcat cgtcaggccg ccgggatagg taaacgtggc atcccagccg ttgacgttgt
     3061 cggtaaaacc ctgcttgcgg taatcggcgc ggcattgcag ctcgaacggc tgcgtaccca
     3121 cctcggggca gccccagttg gcctggtcca ggttgtgcac gccccagttg cagatgaagc
     3181 ccacgcagta gtcccagatc aggtaccagt cgggccaggc gacgcggccg ggattgtacg
     3241 gcttgtcggg cgccggccct cgccacatgg cccagtcaaa cccgtccggc accggctgcg
     3301 ggtcaagcgg gccgtcgtac ttcggcacat acaccgggcc tggtgcagcc acttcaagcg
     3361 tatgaatctt cccgagatac ccgttcctgg cgagcgtgca cccccgctga aagtgttcaa
     3421 gcgagcgctg ccatgtgccg gtctgaaaga cccgtccggt tgcacggacc gtgttgcgaa
     3481 tcaactggct ctcctcaaat gagattccca gcggtttttc gcagtagatg tctttcccgg
     3541 cctttgctgc ggcgaccgcc atcacggcgt gccagtggtc ctgcaccgca atggaaaccg
     3601 cgtcgatatc ctcccgggcc agcacctccc ggaaatcgcc gtacatggcg caaccctggt
     3661 cgccgttggc ttcatcaatg accgctttgg tgctttcgcg ccggtcccgg tacacatcgc
     3721 agaccgcaac gacccggcat gccttttgcg ccaggaaatc cttcaggttg tgccgcccca
     3781 taccgcccac gccaatgaat gcgacggaca ctgtctcgct gggcggcggc gcgtcttctg
     3841 cgccccagac ggatgcgggg aggatggtgg gcgcggcggc ggcggctcct gctgccacgg
     3901 aatagccaag aaatgtgcga cgattgagtt tccgatgcat atccacctca cttccgcagg
     3961 tagtggcgcc acgccggaac gcgcagactg tgcggcttcc ggcgcttgac ccccgtggcc
     4021 catggcaaaa aacgcacccc cgtgttgtac ttcttatccc ccgccagagt caacacggca
     4081 ccgcgcggcg gcacattcga agatttccgc cggtcccgat tctaactgcg cgcgttttgc
     4141 ttttttggac tttggcgaag cctttttggg gcccgggctg tctgcggctc gcccaaacac
     4201 gacaacg
//
LOCUS       DAOPBQ010000329         4207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17324_length_4207_cov_3.722543, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000329 DAOPBQ010000000
VERSION     DAOPBQ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17324_length_4207_cov_3.722543"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKW60_10580"
     CDS             complement(<1..654)
                     /locus_tag="PKW60_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99921.1"
                     /translation="MEQTARFTHLDGAVLIGYFVVVMVFGLWVARRVRSSNRYFLGDR
                     KLRWWIMVGQAFGTGTHAEHPVAQAGAVAEFGFATIWYQWKNMLITPFYWLLAPWYRR
                     SERTTIGEMVEDRYGRRLAMVYTLFAIAYFVFNQGAMLKGAGKAISVATGGQAVSPNE
                     VVFAMTAAFIVYSFFGGLVASAYTDFVQSFLIILLSVMLIPAGLSRVGGFAGLHDSLP
                     "
     gene            976..2298
                     /locus_tag="PKW60_10585"
     CDS             976..2298
                     /locus_tag="PKW60_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPJ99922.1"
                     /translation="MTETADLTERRPWYEGLQRYHYLVLIVACLGWSFDTMDQWLYVF
                     VKQHALRELLHGTVPDSGLNTYIGYAQTFMILGWATGGLLFGMIGDRLGRTRTMAVTI
                     LVYALFTGLSGLSQNWWQFALLRFLTGLGVGGEFAAGAALVAESFPAHSRSTALSLVQ
                     ASSTLGNVAAGLINLIISTYMPVEQAWRWVFAIGIIPALLVVVIFAFIREPEAWQVAR
                     DRAREAKARGERRQVGSILALFTERTTRRNTLVGVALAAIGVIGFWGISTWTPELLRA
                     TLSVQNLDPSAIERRVSYAGMLQNFGGMFGALTFGWLARRIGRRPSFLIALLGCAIEI
                     PLAFRFATTYHMALLFFFGMGFVLMLLLGGLTVYFPELFPTRLRATGTGFCYNVARYL
                     SAPSPTFFGQLSAAIGIQKAALTVSSAFVLGIFVLPFAPETKGKPLPE"
     gene            2568..3830
                     /locus_tag="PKW60_10590"
     CDS             2568..3830
                     /locus_tag="PKW60_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPJ99923.1"
                     /translation="MTTPTHDTAPWLTEAAPEELRRIVVALYRAHRLISAITDLDVLL
                     ERILDESMQVAQAEACSLLLYDRASEELYFRVALGDSGDLQTLKREIRLKLGQGTAGA
                     AAAMRECINVTDVSQDSRFYEVADQRSNFRTRNLLAVPLIDRDELVGVIEVLNKVGGT
                     HFTTVDLHVMEIFSALTASALANAQLIEDNLRAERLAALGQAVAGMSHHTKNILAGMT
                     SSVDLIDQGLSQGSHEFLHQSWPVFKRCTERIANFVEDMLAYSKPRVPMRRPCDLNGI
                     ILDACETFRGLLVVKEVTLDIDTSRLKRQPCLDERAIYRCLLNLLTNAADAVPRAGGT
                     IHVAAYPGTDGQVCLEVIDNGTGIPEAIRESVFDPFFSTKGSDGTGLGLAVTKKIVEE
                     HGGSIELDTAPGGGALFRIRLPWEHTTK"
     gene            3837..4100
                     /locus_tag="PKW60_10595"
     CDS             3837..4100
                     /locus_tag="PKW60_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99924.1"
                     /translation="MVAKNKSAGLEYVGWRFWFVVFAVVAAPATYLSFQLVTERTTPL
                     VPVAMGAIGAAVGAGVVSWAVNSVLQYQTRKRHLAARKKAKKR"
BASE COUNT          819 a         1290 c         1268 g          830 t
ORIGIN      
        1 cggaaggctg tcgtgcagac ccgcaaaccc gcctacccgc gacaaaccgg ccgggatcag
       61 catcaccgag aggaggatga taagaaagga ctgcacaaaa tcggtgtagg ccgaggccac
      121 caggcctcca aagaaactgt acacaatgaa ggccgccgtc atggcgaaca ccacctcgtt
      181 cggtgagacg gcctggccgc ccgttgccac cgagatggct ttgcccgcgc ctttcagcat
      241 ggccccctgg ttaaacacga aataggcgat ggcaaagagc gtatacacca tggcgagccg
      301 ccggccgtat cgatcttcga ccatctcgcc gatcgtcgtc cgttcactgc gccggtacca
      361 gggcgccagc agccagtaaa acggcgtgat cagcatgttc ttccactggt accagatggt
      421 ggcgaagccg aactcggcca cggcccccgc ctgggccacc gggtgttccg cgtgcgtgcc
      481 ggtgccaaac gcctggccca ccatgatcca ccagcggagt ttccgatcac ccagaaagta
      541 gcggttgctg ctccggaccc gccgcgccac ccatagcccg aacaccatga ccacaacgaa
      601 gtagccgatc aggaccgcgc cgtcgagatg ggtaaagcga gctgtctgct ccattgtgtg
      661 aattccccga gatgtcgtaa gcccgtgcga ctgcgccctc tggacaactg aatgcatccc
      721 ccggcagcgg acgctcccct gggctgcgat ttgaccacaa tacctgtctg agattcaacg
      781 agtcgccacg gtttacgcca tgatttgcgg cgctccgagc gcgcagcccg aacaaaaaca
      841 gcatgcgcgc ggcaccgcca caaagacaaa ggagctggtt gtttccgttc ttccatctgg
      901 tttagacttc tgccggcttc ttcgcacatt cgtgtcgcct ggggatcggg gaattgcgag
      961 gttgaagggg gtatcatgac agagacggca gatttaacag agcggcgccc atggtacgag
     1021 ggcctgcaac gctaccacta tctcgtgttg atcgtagcct gcctgggctg gtcttttgac
     1081 accatggacc agtggctcta cgtctttgta aaacaacacg ccctgcgcga actccttcac
     1141 gggaccgtgc ccgactcggg gttgaatacc tacatcggct acgcccagac ctttatgatc
     1201 ctgggatggg caaccggcgg tctgctcttt ggcatgatcg gcgatcggct gggccgaact
     1261 cgaacgatgg ccgtcactat tctggtctac gccctgttta caggcctcag cggcttgtcc
     1321 cagaactggt ggcagtttgc gctgctgcgt tttctgacgg ggctgggcgt gggcggagag
     1381 ttcgccgccg gcgccgcgct cgtcgctgaa tccttccccg cacattcccg cagcacggcc
     1441 ttgagcctgg tccaggcctc ctccactctg ggtaacgtgg ccgcgggcct tatcaacctg
     1501 atcatctcca cgtacatgcc cgtagaacag gcttggcgct gggtattcgc catcggcatc
     1561 atccccgcct tgctggtagt ggtcattttt gccttcatcc gggaaccgga agcctggcag
     1621 gtggcgcgcg accgcgcccg cgaagccaaa gcccggggcg aacgccgcca ggttggctcg
     1681 atcctggccc tgttcaccga gcggacgacc cgccgcaaca cccttgtggg ggttgcgctt
     1741 gccgccattg gcgtcatcgg attctggggt atcagcacct ggaccccaga gctgctccgc
     1801 gccaccctca gcgtgcagaa cctggatcct tcagccattg aacgccgtgt cagctacgcg
     1861 ggcatgctgc agaattttgg cggtatgttc ggcgcattaa ccttcggctg gctggcgcgg
     1921 cgcatcggcc ggcgtccgtc tttcctcatc gccctgctgg ggtgtgcgat cgaaatcccc
     1981 ctggcgttcc ggttcgcgac gacgtaccat atggccctcc tcttcttctt tggcatgggc
     2041 ttcgtcctga tgctgttgct gggcggcttg acggtctatt tccccgagct gttccccaca
     2101 cgcctgcggg ccaccggcac gggcttctgc tacaacgttg cgcgctacct gtctgcgcca
     2161 tcgcccacct tcttcgggca actcagcgcg gcgattggca tccagaaagc ggcactgacc
     2221 gtttcgtcag cctttgttct ggggattttc gtcttaccct tcgcaccgga gaccaaaggc
     2281 aaacctttgc ccgaatagcc ggggatgcgc gatcctggat cacgtccaaa ggcgcagggt
     2341 ctttcagatg tgccggagtt gacgtatgcc agcccggctc tccactcatg gatcgtgatc
     2401 cggcactcca gggggcggct ttgacgagaa agggcaaagg gcaatggccg tgcgctcagt
     2461 attcgcatgt gcgggtccgc ttgcagcagg tttgcggggt gatttatgat gcagtgtcgg
     2521 caaaatcacc aggagagaga atccaagcgt gaaggtttcg tccgcctatg acaacgccca
     2581 cgcatgacac ggcaccctgg cttaccgaag ccgctccgga agagcttcga cggattgtgg
     2641 ttgctctgta ccgtgcgcat cgtctgatct cagcgatcac ggacctggac gtgcttctgg
     2701 agcgcattct ggacgaaagc atgcaggtcg cccaggcgga agcctgctcg ctgctgctgt
     2761 acgaccgggc gtcagaagag ctctatttcc gggttgcgct gggcgacagc ggggatttgc
     2821 aaacgttgaa acgggagatc cggttgaagc tgggccaggg taccgccggc gccgccgcgg
     2881 ccatgcggga atgcatcaac gtgacggatg tcagccagga cagccggttt tacgaagtgg
     2941 ccgaccagcg ttcgaatttt cgcacccgca accttctagc cgttccattg atcgaccggg
     3001 atgagctggt gggcgtgatt gaagtgctca acaaggtggg tgggacccat ttcactacgg
     3061 tcgatttaca cgtcatggag atcttttccg cgcttacggc ctccgccctg gccaacgcac
     3121 agctcatcga ggacaatctg cgcgccgaac ggctggctgc cctgggccag gccgtcgcgg
     3181 ggatgtcgca ccataccaag aacattcttg cgggcatgac gagcagcgtc gatctgatcg
     3241 atcagggtct gagccagggc agccacgaat tccttcatca gagctggccg gtgtttaagc
     3301 gatgcaccga gcgtattgcg aactttgtgg aagacatgct ggcctattca aagccgcgcg
     3361 tgcccatgcg gcgtccctgc gatttgaacg ggatcatcct ggacgcctgc gagacattcc
     3421 ggggcctgct ggttgtcaag gaagtaaccc tggacatcga cacatcccgc ctgaaacggc
     3481 aaccctgcct cgacgaacgc gccatatacc ggtgtctgct gaatctgctg acaaacgcag
     3541 ccgatgccgt tccgcgagca ggcggcacga ttcacgtagc ggcctatccg ggaacggatg
     3601 gccaggtctg cctggaagtg atagacaacg ggaccgggat tcccgaagcc atccgggaaa
     3661 gcgtctttga tccgttcttt tcgacaaaag gctcggatgg cacgggcctg ggtctggccg
     3721 tcacgaaaaa aatcgtggag gaacacgggg gcagcatcga gcttgacacc gcgcccggag
     3781 ggggcgcact gttccgaatt cgtctcccct gggaacacac aaccaaatga ggctgaatgg
     3841 tggcgaagaa taaatcagca gggcttgagt acgtcggatg gcgcttctgg ttcgtagttt
     3901 ttgccgtggt ggcggccccg gcgacctacc tgagctttca actcgtgaca gaacggacga
     3961 cgccgctggt gcccgtcgcc atgggcgcga ttggcgcagc cgtcggagcc ggcgtggttt
     4021 catgggccgt caattccgtg ctgcaatacc agacccgaaa acgccacttg gcggcgcgca
     4081 aaaaggcgaa gaaacgttga acaggccaca gaggcggaca gcgtcacagg catggcaaat
     4141 ctgcaagata tcgaaacgta tctgattgag catccagacg accatgcgca acggtggcgg
     4201 ctggcaa
//
LOCUS       DAOPBQ010000330         4194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17383_length_4194_cov_3.606185, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000330 DAOPBQ010000000
VERSION     DAOPBQ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17383_length_4194_cov_3.606185"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKW60_10600"
     CDS             <1..505
                     /locus_tag="PKW60_10600"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HPJ99925.1"
                     /translation="AQAIIDELTYRQQQIGLPMQAYAARITAFILETLGTPIALSGSV
                     LQVNGVSVAVAEACNGIRMLFALTLVCFLYAFSTRLSTQTRILIVLMSPFVAIAANVL
                     RLIPTVWLYGYTNSHTADMFHDISGWAMMPVALFGLFSAGALLRWTFAKDSDQPEEAR
                     QSVASST"
     gene            542..1213
                     /locus_tag="PKW60_10605"
     CDS             542..1213
                     /locus_tag="PKW60_10605"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase-associated EpsI family protein"
                     /protein_id="HPJ99926.1"
                     /translation="MRLLAPALSLALLAAIVVDQGYRHQVPSGAETYHAIVREAAKTI
                     PYQLGNWVGVDSEISRGALQILDANVALSRSYRQVGTNRAGTLLIVQCADARSLLGHY
                     PPVCYPSQGWSQISAKPHDIEIDGVALRATEYIFAYETLAQFTRLAVLHFTVLPDGRT
                     APDMDPLERVARERKTKFLGGASLQFVSDASLSEQDRDEIYDFLFQAAKAWIEEVQSG
                     TQRTS"
     gene            1210..3681
                     /locus_tag="PKW60_10610"
     CDS             1210..3681
                     /locus_tag="PKW60_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99927.1"
                     /translation="MNQQRLPSVPNQSGYPASAKAPGTSPEQNPLLLLHRLLRGRYAA
                     AIILAILLGAAGAVGGYIALALEYQSTAQIRIAPVMPKILFESEQSSVLPMFDSFVQT
                     QVSLIQSRRVVDMAMQGSTWKSVGGEYSLPRVADFMEQLSVTHPRNTEIILVTFTDHE
                     PRTAQAGAKAVVDAYMNIYGGEDLNTATQRFELLEERAKALGNEIDGYQAQMYEIANE
                     YGSNALDGLYAFEAEELQKLQTELESAKIELATFESASKEGLLPNIGKDPSRLSVEEI
                     ATVDSWMKQLLDTRKQLRWSMDEIATRYKEPEQRPEYRLARERLSTQEDEIANYAALY
                     REQAAANAGLPMAGANDAETLKANLELRVQRLQQLFDEKRERTLALGRQNLQIEKLRS
                     QIARAQDSLDKTNARIEQLSIESAIGGRIDLLAEADMPTMPSNARRRIQFAILGGMGG
                     AGLGVALVMLLGLVNKRIRDVVDAEATRPRLLGVLPELPERLSDPAEAMLAAQSVHHI
                     RTLLQIQAGTKQPLSLTVTSATSGTGKTSLVLSLGLSFAAAGSRTLLIDGDVIGTELT
                     RRTRAFGWRMLGQVTSEFDIISEEALQKAIESSFEVIESSFESNSGSRQSVPPIANEE
                     LADGLSIHSEPKYGLSDALRGEPIENCISSWGLPNLSILSAYGTSGTAANGFSPASAR
                     ELLDQLSDTFDVVLVDTGPIPGQTESSVMAACTSGVIMMVARGDHDADVRKALHHLEL
                     IDAQIAGLVFNRARNRDMERSGYSSSRTSARSASPAPEAGEEARDLTVEAKAYARFDP
                     LTQAVLCMSMDASIARGIVRDESSS"
     gene            3693..>4194
                     /locus_tag="PKW60_10615"
     CDS             3693..>4194
                     /locus_tag="PKW60_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99928.1"
                     /translation="MRKRRLLIVGGTFLVVALVAGGAVYYRHTSMNRQALACRARGLE
                     AAQAGDYTTALDQVGRYLQRYGMDEDVEALFAYATARRNIPAANNKHLQQAMAVFARI
                     LELDPGHAEAQNALLDLYTSVGFTEETLVLADKILPAPANGVPLEKLLAVEHIPALRA
                     KALTLAK"
BASE COUNT          908 a         1264 c         1214 g          808 t
ORIGIN      
        1 ggcgcaggcg attatcgacg aactgaccta ccgccagcaa cagatcgggc ttcccatgca
       61 agcctacgcg gcccgaatca ctgcttttat tctggaaacc ctgggcaccc ccatcgcctt
      121 gagcggcagt gtgctccaag tcaatggcgt ttcggttgcc gtggcggagg catgcaatgg
      181 cattcgcatg ctcttcgccc tgaccctggt ctgctttctg tacgcattca gcacgcgcct
      241 cagcacacag accaggatcc tgatagtcct gatgagcccc ttcgtagcca ttgccgctaa
      301 tgtcctgcgg ctgattccga ccgtgtggct gtacggatac accaatagcc acacggccga
      361 tatgttccac gatatcagcg gctgggccat gatgccagtc gcattgttcg gtctgttcag
      421 cgcgggtgcg ttgctccgat ggaccttcgc caaggacagc gaccagcccg aggaggcgcg
      481 ccagagcgtg gcgtccagca cgtaaaccgg ttacggtttg agaagacgga gagccatttt
      541 gatgagatta ctggcaccgg cgctttcgct cgcgttactg gcggccatag tggtcgacca
      601 ggggtaccgc caccaggtgc cttcgggagc ggaaacctat catgccatcg tccgcgaagc
      661 ggccaagacg atcccctacc agctcggaaa ctgggttggg gtcgattccg agatttcccg
      721 gggcgctctt cagatcctcg acgccaacgt ggccctgagc cgctcatacc ggcaggtcgg
      781 caccaatcgg gcgggtacct tgctcattgt ccaatgcgcc gatgcccgaa gcctcctggg
      841 ccattacccg ccggtatgtt atccctcgca ggggtggtcg caaatctccg ccaagccgca
      901 cgatatcgaa atagatggag tcgctctgcg cgcgaccgaa tacatcttcg cctacgaaac
      961 cctggctcag ttcacccggc ttgctgtgct acactttacg gtgctcccgg acgggcggac
     1021 cgcgcccgat atggatccgc tcgagcgcgt cgcgcgggag cgaaagacta aatttctggg
     1081 tggtgcaagc ctccaatttg tatcggatgc gagcctttct gagcaggatc gcgatgaaat
     1141 ctacgacttt ttgttccagg ctgcgaaagc atggatcgag gaagtacaat cgggaaccca
     1201 gagaacatca tgaatcaaca acgattgcca tctgtaccca atcagtcggg ctacccggcc
     1261 tcggccaagg cgcccggcac cagccctgaa cagaacccgc tgcttctgct tcaccggctg
     1321 ttgcggggaa ggtatgctgc ggcgatcatt ctagcgatct tgctcggagc cgccggagcg
     1381 gtgggcggtt acatcgcgct ggcgctggaa taccaaagca ccgcccagat ccgcattgcc
     1441 ccggtcatgc ccaagatcct ttttgaaagt gaacagagca gcgtgctgcc tatgttcgat
     1501 tcgtttgtgc agacgcaggt ttcactaatc cagagccgcc gcgtcgttga tatggccatg
     1561 cagggctcga cctggaaaag cgtcggcggc gagtattccc tcccgcgggt cgccgatttc
     1621 atggagcaac tgagcgttac tcatccccgg aacaccgaga tcatcctggt gacatttacc
     1681 gatcacgagc cgcgaaccgc ccaggccggg gctaaagccg ttgttgatgc ctacatgaac
     1741 atttatggcg gtgaagacct caatacggct acccagcgtt tcgagttgct ggaagagcgg
     1801 gccaaagcgc tcggcaatga aatcgatggt taccaggcgc agatgtatga gattgcgaac
     1861 gaatacggtt ccaatgccct cgacggactc tatgcgtttg aggcggagga gcttcagaag
     1921 ctgcaaaccg agcttgagtc cgcaaaaata gaattggcga cattcgaatc cgcgagcaaa
     1981 gaaggcttgc tgccaaacat tggaaaagac cccagccgac tctccgtgga ggagatcgca
     2041 actgtcgaca gctggatgaa gcaacttctt gacacccgca aacagctccg ctggagcatg
     2101 gatgagattg cgacccggta taaagaacct gagcaacgcc ctgaataccg cctggcacgg
     2161 gaacgcttga gtacccagga ggacgagatc gcgaactacg cggcgttgta tcgggaacag
     2221 gccgcggcca atgccggatt gcccatggcg ggagccaacg acgcggaaac cctgaaggca
     2281 aacctggaac tgcgcgttca gcgcctgcaa cagcttttcg atgaaaaacg agagcgtacc
     2341 ctggcgctgg gccggcaaaa cctccagatc gaaaaactcc gcagccagat tgcccgcgca
     2401 caggacagcc tggacaaaac caacgcgcgc atcgaacaat tgagcattga atctgccatt
     2461 ggcggccgta tcgatctact cgccgaggcg gacatgccta cgatgccgtc taatgcgcgc
     2521 cggcgcatcc aatttgccat tctgggcggc atgggcggcg ctggcttggg tgtcgccctg
     2581 gtcatgctgc tcggcctggt gaacaagcgc atacgcgatg tggtggatgc ggaggccacg
     2641 cgcccgagac ttctgggggt cctccccgaa cttcccgaga ggctttccga cccggcggag
     2701 gccatgctcg ccgcgcagag cgttcaccac atccgaacgc tccttcagat ccaggcgggc
     2761 acgaaacagc ctctttcgct tacggtcacc agcgcaacaa gtggtacggg aaagaccagc
     2821 ctggttttgt ccctgggttt gtcgttcgcg gccgcaggaa gccgcactct ccttatcgac
     2881 ggcgacgtga taggtacgga actcacacgt cgaacacgcg cctttggatg gcggatgctg
     2941 gggcaggtga ctagtgaatt cgacataatc agtgaagaag cactgcagaa ggcgatagag
     3001 tcatctttcg aggtgataga gtcatctttc gaatcaaatt ccggctccag acaaagtgtt
     3061 cctccaatcg ccaacgaaga attggcggat ggcctatcga ttcacagcga accgaagtac
     3121 ggactaagcg acgcccttcg cggcgaaccc atcgaaaatt gcatcagctc ctgggggttg
     3181 cccaacctca gcatactttc ggcgtacgga acgtctggga ctgccgccaa cggcttttca
     3241 ccggcatccg cccgcgagct tctggaccag ctctccgaca ccttcgacgt tgtgctcgtc
     3301 gatacggggc cgatacccgg tcagacggaa tcgtccgtaa tggccgcctg taccagcggg
     3361 gtaatcatga tggtcgcgcg tggcgaccac gacgcagatg tgcgcaaagc cttgcaccat
     3421 ctggaactga tcgacgcgca gattgccgga ctggtcttca accgtgcccg caatcgagat
     3481 atggagcggt cgggttactc ctcgtcgcga accagtgcaa ggtctgcctc cccagcgccg
     3541 gaggccggcg aagaagcgcg cgatctcacg gtcgaggcga aggcttacgc ccggttcgac
     3601 ccactcacgc aagctgttct ctgcatgtcc atggatgcat ccattgccag gggaatcgtt
     3661 agggacgaat ccagctcgta aaaaggtagg tcatgcgaaa aagacgtctg cttatcgttg
     3721 ggggaacatt tcttgttgtc gcgctggtcg cgggaggagc cgtatactac cgccatacct
     3781 ccatgaacag acaggcgctg gcgtgccgtg cgcgcggtct ggaagccgct caagccggcg
     3841 actataccac agccctcgac caggtgggcc ggtatttaca gcgatacggc atggacgagg
     3901 atgtggaggc tctattcgcc tacgccacgg cgcgccggaa catccccgca gccaacaaca
     3961 agcatctgca gcaggccatg gctgtctttg cgcgaattct tgagctggat cccggacatg
     4021 ccgaagccca gaacgcactc ctcgacctgt acacgtccgt ggggtttacg gaagagaccc
     4081 tggtgctggc cgacaagatc cttcctgccc ccgcaaatgg agtcccactc gaaaaactcc
     4141 tggcggtcga acatattccg gcacttcgcg caaaggcgct caccctcgcc aaga
//
LOCUS       DAOPBQ010000331         4159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17550_length_4159_cov_4.009990, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000331 DAOPBQ010000000
VERSION     DAOPBQ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17550_length_4159_cov_4.009990"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..804
                     /locus_tag="PKW60_10620"
     CDS             406..804
                     /locus_tag="PKW60_10620"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPJ99929.1"
                     /translation="MTKTKRELVIDVANKLGITQSTVARIIEGAFEAISLALAEGERW
                     ELRDFGVFEVKTRASRIGRNPRTGEQVPVPERKVVTFRPGKKMKELVCAPGPADSAAP
                     QLPDEPAQEPDRPGDLAESSSKDATETPLF"
     gene            952..1650
                     /gene="tsaB"
                     /locus_tag="PKW60_10625"
     CDS             952..1650
                     /gene="tsaB"
                     /locus_tag="PKW60_10625"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPJ99930.1"
                     /translation="MIIVAGDTSTRTNTVAVCNDDLILAETVVDCGRTHAERLLDTIT
                     WALDEAGVPLTEVNVLAISTGPGSFTGLRVGLAAWKGLAFGLGIPLVGVPTLAAMSRL
                     PGMDNGVCCPMLDARMGEVFAAAYRFESGARSPVLADCVAPVEKVAEALRAYGSVTLF
                     GDGTGVYDARIRASLPGCRMLPRMFWAPRASAVAAEARDMLQAGWNTEAADVRPVYLR
                     KSQAEQNRERAGHP"
     gene            1647..2153
                     /gene="rimI"
                     /locus_tag="PKW60_10630"
     CDS             1647..2153
                     /gene="rimI"
                     /locus_tag="PKW60_10630"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPJ99931.1"
                     /translation="MTSPNAPRVEFTALAETDLDAILDIELEAYPEPWSREMFREEIR
                     NNRSYFYVMQLNGELAGYGGFWLVLDEAHITSVTVRTDLRGQGLGRTLTRHLLDAAKE
                     AGAHIATLEVRESNMRAQNLYKTFGFRQIGRRKGYYSKSGEDAIVMLLELGPDESSAQ
                     DMSGEAGA"
     gene            2171..>4159
                     /locus_tag="PKW60_10635"
     CDS             2171..>4159
                     /locus_tag="PKW60_10635"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HPJ99932.1"
                     /translation="MAGNLNQDAFRSYRSRGRNAKNRRISWGVPVGGIGTGCIELGND
                     SRFRNITINNNRDKASRIALSPRAFIALRTVRQGRVQTRFLQPDCALPFADAGIIPTF
                     TPVNELTWRALYPCSYYTLNAKESTIEATWRALAPVVPHDVSSSNIPVMFFSLAVRNP
                     ALTPVKVSFVFNWENLCGCERSTVPTDRGAFTPVPYRNELSEPRFQGLSFGKTDRFLS
                     NADGNYALLAQPQLGQDISVLGWNADDAADLDAFWQQFHYDGRLANQVSRNETAHSAA
                     VCCTRELEPYQECRFLLALSWYCPRFVVDGVDLGNKYAEVFRNAVEVGQGALRNVNFY
                     VNAVESWQNSLLTSSLPRWFSRMLINSCATFSTNSLLTRRGEFALFETPEDPMTGCLD
                     KRLYSSLATLLLFPELEEAEFYALAGAMPAAEPGRCVRYLGRMTLHAPGDGTRREELV
                     DLGPKFVLLACRNLRFTGNRRMAETLFPRLRAVMRHIANMSPQNDGLPRQTGLSTMYD
                     DWATDGVNSYTSSLWIAAMRAYSALAAYLGDSAEAARYATLAEHAVERFESLLWNEEE
                     GYYTFCAPGAESSERLADWHLGCHTGQLAGEWYAQWLGLGPLFEPRRVVRAMSAIQRL
                     NERKFCLARGTLPDGYPCENPPETGVEPQTEHSWPGILL"
BASE COUNT          835 a         1171 c         1355 g          798 t
ORIGIN      
        1 acccgcccct ggagccgcga ggtcggatct cctgcccagt ctgagaccca ggcatgtgcg
       61 gcttcatgca ccgacaggca gagcaggaga cagacatagg cggcgaatat ttcagcgatg
      121 tgcgggctca taggtcctta taaaggccaa gttatcgaag aggtattgta aaagacggca
      181 tcggaaagaa tcaaacgttc cgccgtttca tttccactgg ggcggtttcg gggtgtgtag
      241 ccggttgact tcacgggcaa tcgtcgataa gatgactata cgaaaagatt tgcaaggaat
      301 tgcatgcttc cgtataatag cctgtgagcg aaatcgcgac gcagcaagtc agcccgtcaa
      361 gggagcgagc gtcccggcca gggttggcga ggaggattgg attccgtgac taaaaccaag
      421 cgcgagttgg taatcgacgt ggccaataag ctggggataa cccaaagcac cgtggccagg
      481 attatcgaag gagcctttga ggccatctcg ctggcgctcg cggaagggga acgttgggaa
      541 ttgcgtgact ttggcgtgtt tgaggtgaaa acacgcgcct cgcgcattgg acgaaatccg
      601 cgaaccggcg aacaggtccc tgtgcccgag cgaaaagtcg taacgttccg gccgggaaaa
      661 aagatgaagg agctggtctg cgcgccgggc ccggcggact ctgccgcccc gcagctgcct
      721 gacgaaccgg ctcaggaacc ggacagaccg ggagacctcg cggagagttc gtcgaaagac
      781 gccacggaaa ctcctttgtt ctaagggcgt tacgctagaa tcggctacag gccgcagggt
      841 tgaaatacgt cttgcggcct tttatgtgtc tgggcagcca ggaaaatcgg gcggcagtag
      901 tgcctggcca aatcgttgac ctgtgagcgc ttagccccgt atgatccaaa tatgataatc
      961 gtagcgggag atacatccac ccgcaccaac accgtcgcgg tgtgcaatga cgacctgatc
     1021 ctggccgaga cggtggtgga ctgtgggcgc acgcacgccg agcgtctgct ggacacgata
     1081 acgtgggcat tggatgaggc cggagtgccg ctgactgagg ttaacgtgct ggcgatcagc
     1141 acgggcccgg gctcattcac gggcctgcgg gtgggtttgg ctgcgtggaa aggactggcc
     1201 tttggcctgg gcatccccct ggtgggggtg ccgacgctgg cggccatgtc gcgtctgccg
     1261 ggaatggaca acggcgtctg ttgtccgatg ctggatgcgc gcatggggga agtattcgcg
     1321 gccgcatacc ggtttgaatc gggagcgcgc agcccggtgc tcgcagactg tgtcgcgccc
     1381 gtggaaaaag tggcggaagc gttgcgtgca tacggctctg taacgttatt cggagatgga
     1441 acaggcgtat atgatgcgcg cattcgcgcg agtcttcccg gctgccgcat gcttccgcgg
     1501 atgttctggg cgccgcgggc atccgccgtt gccgcggaag cccgtgacat gctgcaggcg
     1561 ggatggaata cggaagcggc ggacgtacgc cccgtctatc tgaggaaatc gcaggccgag
     1621 caaaatcggg aacgagcggg ccatccatga cttcaccgaa tgcaccgcgc gtggaattca
     1681 cggcacttgc ggaaacggat ctggatgcga tcctggacat tgagctcgag gcgtatccgg
     1741 aaccgtggtc acgcgagatg ttccgtgagg aaatccgcaa caatcggtcg tatttctacg
     1801 tcatgcaact gaacggtgaa cttgccggct atggcggatt ctggcttgtg ctggacgagg
     1861 cccacatcac cagtgtcacg gtccgcacgg atttgcgcgg gcagggactg ggccggacgc
     1921 tcacgcggca cctgctggac gcagcgaaag aagcgggcgc acatatcgcg acccttgagg
     1981 tgcgcgaatc gaacatgcgg gcccaaaatc tgtacaaaac gttcggattc cgccagatag
     2041 ggcggcgaaa aggatattac tcaaagagcg gtgaggacgc cattgtgatg ctgctggaac
     2101 tcggcccaga cgaaagctcc gcccaggaca tgagcggcga ggcgggcgct tgagatggaa
     2161 gcggcgtagc gtggcgggaa acctcaacca ggacgccttt cgatcttatc gctcgcgggg
     2221 ccgtaacgcc aaaaaccggc gtatctcgtg gggcgtgcca gtgggaggca tcggcacggg
     2281 ttgcatcgag ctggggaacg acagccggtt tcgcaatatc accatcaaca acaaccgcga
     2341 caaggcctct cgtatagcac tgtcaccccg ggcttttatc gcgttgcgaa ctgtccgcca
     2401 gggacgcgtg cagactcgct ttctgcaacc ggactgcgcc ctgccctttg ccgacgcggg
     2461 catcattccg acgtttaccc ccgtcaacga actcacgtgg cgcgcgctct atccctgctc
     2521 gtattacacg ctgaatgcca aagagtcgac cattgaggcg acgtggcgcg cactggcgcc
     2581 cgtggttccc cacgacgttt cctcgtcaaa tatcccggtc atgtttttca gcctggcggt
     2641 gcgcaacccg gcgcttacgc cggtcaaggt ttcgtttgtc ttcaactggg aaaatctctg
     2701 cggctgcgaa cgcagcaccg tgcccacaga ccggggcgct ttcacaccgg taccgtatcg
     2761 aaatgagctc tcagaacccc gctttcaggg cttgagtttt ggcaagacgg accggtttct
     2821 gagcaacgcc gacggcaatt atgcgctgct ggcgcaaccg cagctgggcc aggatatttc
     2881 ggtgctgggc tggaatgccg atgatgcggc ggacttggat gcattctggc agcaatttca
     2941 ttacgacggg cgcctggcca accaggtgtc tcgcaacgag acagcgcatt ccgcggcggt
     3001 ctgttgcaca cgtgagctcg aaccgtatca agaatgccgc tttctcctgg cgctgagctg
     3061 gtactgcccc cgcttcgttg tggacggcgt ggatctcgga aacaaatatg cggaggtctt
     3121 ccgaaatgcc gtcgaggtcg ggcagggggc gttgcgtaac gtaaatttct atgtcaatgc
     3181 cgttgaatca tggcagaact ccctactcac ctcgtcgctt ccccgctggt tttcgcggat
     3241 gctgatcaac agctgcgcga ccttttcgac caacagcctc ctgacgcgcc ggggtgaatt
     3301 cgcgctgttt gagacgcccg aagaccccat gacaggctgc ctggataagc ggctctacag
     3361 ctcgctggcc acgctgctcc tgtttccgga actggaagag gcggaatttt acgccctggc
     3421 aggggccatg ccagcggccg agcctggccg ttgcgtgcgg tacctgggcc gaatgacgct
     3481 tcatgcgcct ggagacggta cgcgccggga ggagctggtt gacctggggc cgaagttcgt
     3541 tctcctggcc tgccggaacc tgcggtttac gggcaaccgc aggatggccg aaacgctgtt
     3601 cccgcggctg cgcgcggtga tgcgtcatat cgcgaatatg agtccgcaga atgacggact
     3661 tccgcggcag acggggctgt cgacgatgta cgacgactgg gccacggacg gggtgaacag
     3721 ctacacatcg agcttgtgga tcgcggcgat gcgggcttat tcggcactgg cggcgtatct
     3781 gggcgattcg gcggaagctg cccggtacgc gacccttgcc gagcacgcgg tggaacgctt
     3841 tgaatcgctg ttgtggaacg aagaagaggg ctactatacg ttctgcgccc cgggcgccga
     3901 aagcagcgaa cgcctggccg actggcatct gggatgtcac acggggcagc tggccggtga
     3961 atggtatgcg cagtggctgg ggctgggccc gctttttgaa ccccgccggg tcgtccgcgc
     4021 catgtcggcg atacagcggt tgaacgagcg gaaattctgt ctggcgcgtg gcacactgcc
     4081 cgatggctac ccgtgcgaaa accctccgga aacgggtgtg gaaccgcaga ctgaacacag
     4141 ctggccggga atcctgctg
//
LOCUS       DAOPBQ010000332         4137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17659_length_4137_cov_3.755267, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000332 DAOPBQ010000000
VERSION     DAOPBQ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17659_length_4137_cov_3.755267"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PKW60_10640"
     CDS             <1..588
                     /locus_tag="PKW60_10640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPJ99933.1"
                     /translation="EAGITEKEVREDVQRRLRVDRLLDEVCTDVTFPTDAEARAHFEA
                     NPEQFRAPERVRASHIVKSIPRGTILDVQATYTQMEEIHAGLMAGLSFESAVRRFSDC
                     PENDGDLGYFARGAMVPEFETVVFGLEVGQISGVFQSPFGYHIAKVYDKVASQERPFE
                     AVAEQVKAALFDARENAAIDAYTARLREKAEIQEV"
     gene            825..1643
                     /locus_tag="PKW60_10645"
     CDS             825..1643
                     /locus_tag="PKW60_10645"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HPJ99934.1"
                     /translation="MRVGLVGCGNIGTDICLALQKGDIPAKIAALTDIDRSRAELFAK
                     SFELSARICDVDENAASVDFLVECADVSAVATAVEAAIRYRRDCLILSLGGLLTAPEL
                     FDTARQHGVKIWLPSGALAGLDGVRAGREGGLERVTLTTRKPPKGLAGAPWLVERGIS
                     LEGLNQPKVVFDGTALEAVKAFPKNVNVAAALSFAGIGPERTRVRVIADPGATANCHE
                     IVAEGAFGRLVATTENQPSPRNAKSSYLASLSGCAELRAAAEAFVLRRSAGAGI"
     gene            1739..2050
                     /gene="rplU"
                     /locus_tag="PKW60_10650"
     CDS             1739..2050
                     /gene="rplU"
                     /locus_tag="PKW60_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPJ99935.1"
                     /translation="MYAVVRTGGKQHKVSQGDVLRVEKLDAPVGDTVELTEVALLSKD
                     DALVVEPDALKSAKVVCEVVRQGRAKKIRVFKKKRKKNYTRTYGHRQSFTELRVRDIQ
                     A"
     gene            2065..2328
                     /gene="rpmA"
                     /locus_tag="PKW60_10655"
     CDS             2065..2328
                     /gene="rpmA"
                     /locus_tag="PKW60_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPJ99936.1"
                     /translation="MAHKKAGGSSRNGRDSTAQRLGVKKFGGEVVRAGNILVRQRGTQ
                     FRPGENVGIGRDDTLFALADSVVQFRHVTKQRKRVDVVPSESK"
     gene            2353..3606
                     /gene="obgE"
                     /locus_tag="PKW60_10660"
     CDS             2353..3606
                     /gene="obgE"
                     /locus_tag="PKW60_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPJ99937.1"
                     /translation="MFVDRVRIRVTGGAGGNGCCSFRREKYVPRGGPDGGDGGHGGDV
                     FFVATPDVSSLIKLRYHAHWKGEAGNHGQGSNRHGKTGAETTIPVPVGTLVRKLGDDE
                     VCCDLAEPGRRFCAARGGRGGKGNARFATSTRRIPRFAERGEPGEEAEYSLELKLLAD
                     VGLVGQPNAGKSTFLARVTAALPKIADYPFTTVTPNLGVVQLDGFRTMTMADIPGIIE
                     GAAEGKGLGHDFLRHIERTRLLLFIIDAGDEDPESALRMLEAELEQYSPVFASRPRLV
                     AVNKIDIPENRARATEFAQTHGAAYPVSAATGEGVGTVLERAWSIVDRLRREEEITSE
                     KDEAAEYTYESPFTVAREGGGFRVDGRPVLRAVAMTDFENSEAVSHLQNKLQRMGLFK
                     ALKRLGARPGESIYIGDTELEYRPE"
     gene            3587..>4137
                     /gene="proB"
                     /locus_tag="PKW60_10665"
     CDS             3587..>4137
                     /gene="proB"
                     /locus_tag="PKW60_10665"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPJ99938.1"
                     /translation="MNIDLSRAKTIVVKIGTSLLTGPTGFDGRVLEEIVKELCRLKRE
                     RNLNLLVVSSGAVGCGMAALGMTERPKRLPLKQATAAVGQANLMHYYETLFRTYGEGL
                     RSAQVLLSASDLDNRGSYLNIRNTLHTLFALNSVIPIINENDSVSIEELKFGDNDTLA
                     ARVAAKIGADLLIILSNVDGLYD"
BASE COUNT          841 a         1139 c         1400 g          757 t
ORIGIN      
        1 gaggccggca ttaccgaaaa agaagtccgg gaggatgtgc agcggcgcct gcgcgtcgat
       61 cggctgttgg acgaagtgtg cacagacgtt acgtttccca cggacgcgga agcgcgcgcg
      121 cattttgagg cgaacccgga gcagtttcgc gcgccggaac gcgtgcgtgc ctctcatatt
      181 gtgaagagca tcccacgcgg caccatactg gatgtgcagg ccacctatac gcaaatggag
      241 gagatccatg cgggattgat ggccggcctg agcttcgaat ctgccgtgcg gcgcttttca
      301 gactgtccgg aaaacgatgg cgatttaggg tattttgccc ggggcgccat ggtgccggag
      361 tttgaaacgg tggtgttcgg cctcgaggtg ggacaaatca gcggcgtatt tcagagcccg
      421 ttcggctacc atattgcaaa agtgtacgac aaagttgctt cacaggaacg cccgttcgaa
      481 gcggtggctg agcaagtaaa agcggcgttg ttcgatgcgc gggaaaatgc ggccattgac
      541 gcgtatacgg cccggcttcg cgaaaaagcg gagatccagg aggtgtagcc ggcaccgccg
      601 gcatccccgc cgggttcgat ccagcctaaa ttgttgtttg actacgcttt tcccggtatg
      661 ctatgctcct gtgactttaa agaacgggca accacgcgcg acgtgtccga tcgtccgata
      721 gcagcagcgc agtcgcattc accctaagtc attgccgcgc aaccggtggc aacacggaaa
      781 gcgatgcttc gcccgcgata gattgcggtc gtaaaatgca caaaatgaga gttgggctgg
      841 tcggttgcgg caacatcggg acggatatat gcctcgccct gcaaaagggc gacataccgg
      901 ccaagattgc ggcgctgacc gatattgatc ggtcgcgggc agagctgttc gccaaaagct
      961 ttgagctttc ggcgcggata tgcgacgtgg acgaaaatgc cgcgtcggtg gattttctag
     1021 ttgaatgcgc ggatgtgtcc gcggtggcga ccgccgttga ggcagccatc cggtaccggc
     1081 gggactgcct gattctcagt ctcggcggac tgttgacggc gccggagctc ttcgataccg
     1141 cccggcagca cggcgttaag atttggctgc cgtcgggcgc actggcgggt ttggatgggg
     1201 tgcgtgccgg acgcgaaggc ggcctggagc gcgtcactct gaccacacgg aagcccccaa
     1261 agggcttggc gggagcgccc tggctggtgg agcgcgggat aagccttgag gggctgaacc
     1321 agccgaaggt cgtttttgat gggacggcac ttgaggcggt gaaagcgttc ccgaagaacg
     1381 tgaacgtggc cgcggccttg agttttgcgg gcattggccc agagcgcacg cgggtccggg
     1441 tgatagccga tcccggggcc accgcaaact gtcatgagat cgtggcggaa ggcgcgtttg
     1501 ggcgcctggt ggcgacgact gagaaccagc cgtcgccgcg taatgcgaag tcgagttacc
     1561 tggcctcgtt gtcggggtgc gcggagttgc gcgccgcggc agaggctttt gtgctgcgcc
     1621 gcagtgcagg cgcgggcata tagcgttgta acgaacagac ggcgggcgcg cggtcggcgc
     1681 cctggcgcgg attttccggt ttcgggaata cgcatggcgg tgttggagat agagcatcat
     1741 gtacgcagtg gtaaggacag gcggcaagca gcacaaggtg tctcagggcg acgtgttgcg
     1801 cgtggaaaag ctcgatgcgc cggtaggcga cacggtggaa ttgaccgaag tcgcgttgct
     1861 gagcaaagac gatgccctgg tggttgaacc ggacgcgctg aaatcggcca aggtggtctg
     1921 cgaggtggtt cgccagggcc gcgcgaagaa gatccgcgtt ttcaagaaga agcggaagaa
     1981 aaactatacg cgcacttatg ggcaccggca gagcttcacc gaactccgtg ttcgggacat
     2041 tcaggcgtag aggaggcagc agacatggca cacaagaaag caggcggcag ctcgcgcaac
     2101 ggtcgcgaca gcacagcgca acgccttggc gtgaagaaat tcggcggtga agtggtgcgc
     2161 gcgggcaata ttctggtccg tcagcgcggc acacaattcc gccccggtga aaacgtgggc
     2221 atcggcagag acgacacgct gtttgcgctc gcggatagtg tggttcagtt ccggcacgtc
     2281 acgaagcagc ggaagcgcgt cgacgtcgtc ccctccgagt cgaagtaact cggggcatcc
     2341 aggtgatgca ccatgtttgt cgatcgcgta cggatacgag tgaccggagg cgcgggcggc
     2401 aacgggtgct gtagcttcag gcgcgaaaaa tacgtccccc gcggcggccc ggatggaggc
     2461 gatggcggcc acgggggcga cgtgtttttt gtcgcaacgc cagacgttag ctcgctgatc
     2521 aaattgcgtt atcacgcgca ctggaaaggc gaagcgggca accacggcca aggctccaac
     2581 cggcacggca agacaggcgc cgagacgacc attcccgtgc cggtcggcac gctggtgcgc
     2641 aagctcggcg acgacgaggt gtgttgtgat ctggccgaac cgggccgccg tttttgtgcg
     2701 gcgcgcggcg ggcgcggcgg gaagggcaac gcacggttcg ccacctcgac acggcggatt
     2761 ccgcgtttcg ccgagcgggg cgaaccgggg gaagaagccg agtattcact ggaactcaag
     2821 ctgctcgctg acgtcgggct ggtcggccag cccaacgcgg gcaaatcgac gttcctcgcg
     2881 cgcgtcaccg cggccctgcc caagattgca gattatcctt tcacgaccgt cacacccaat
     2941 ctgggcgtgg tgcagctcga tgggttccgc accatgacca tggccgacat cccggggatc
     3001 attgagggcg cagctgaagg aaaaggactg ggccacgatt ttctccggca tatcgaacgc
     3061 acccggctgc tgctgtttat catcgacgcg ggcgatgaag accccgaatc ggcgctgcgc
     3121 atgctggagg ctgagcttga acagtacagc ccggtctttg ccagccgccc ccggctggta
     3181 gccgtgaata aaatcgacat ccctgaaaac cgcgcccgcg cgacggaatt cgcgcaaacc
     3241 cacggggccg catatcccgt gtccgcggcg accggcgagg gggtgggtac ggttctggag
     3301 cgtgcgtggt cgattgtgga tcgtttgcgc cgcgaagaag agatcacttc ggaaaaggac
     3361 gaagccgcgg agtatactta tgagtcgcca ttcaccgtcg cgcgcgaggg cggcgggttc
     3421 cgtgttgacg gcaggcccgt gcttcgcgcc gtggcgatga ccgattttga aaacagcgag
     3481 gccgtatccc atctccagaa caagcttcag cggatggggc tgttcaaggc attgaaacgg
     3541 cttggcgccc ggccgggcga atccatctat atcggagata cagaacttga atatcgacct
     3601 gagtagagca aagacaattg tcgtcaagat cgggacctcc ctgctgacgg gtcccacggg
     3661 cttcgacgga cgcgtcctgg aagagatcgt aaaggagctg tgccggctca agcgcgagcg
     3721 aaacctgaac ctcctggtgg tttcgtcggg ggccgtcgga tgcgggatgg cagcgctcgg
     3781 aatgacggaa cggccgaaac ggctgccgct gaagcaggcg actgcggcgg ttgggcaagc
     3841 gaatttgatg cactactacg agacgctgtt ccgaacctac ggcgaagggt tgcgatccgc
     3901 tcaagtgttg ctctcggcat cggacctcga taaccggggc tcctatctca atatccgcaa
     3961 cacgctccat accttgttcg cgctcaacag cgttatcccg atcattaatg aaaacgactc
     4021 cgtatccatc gaggagctta aatttggcga caacgacacg ctggcggccc gtgtggcggc
     4081 gaaaatcggc gccgatctgc tcatcatttt gagcaatgtg gatgggttgt atgacag
//
LOCUS       DAOPBQ010000333         4135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17669_length_4135_cov_3.144608, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000333 DAOPBQ010000000
VERSION     DAOPBQ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17669_length_4135_cov_3.144608"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..>4135
                     /locus_tag="PKW60_10670"
     CDS             27..>4135
                     /locus_tag="PKW60_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HPJ99939.1"
                     /translation="MPYFGDEVFTYRQPDGSTFEVRLYGDEFFAYQRTADEGREVVLD
                     EATGFWCYARLSADGRSFVSTGVPVTTLAKSAPETNKALAISAGAAQPNATLPYDVII
                     SRVKAEREALTNAARDYLAGTGIPYEVQPKAATGTIIGLCILVDFPDDVATISQAQVE
                     RFLNQPSDYTEFGNACSVNEYFHIQSNGLLNYVNVVTPWIRMPHPKIYYDDNSINSSK
                     PGELLSEARSILSQQGYDVNAYPAVNVFYAGNCLSGWRRGLWPHSAGNYQMTAMGGEL
                     ALGTFCHENGHMICGFPDLYSYTAAYLGVSGSLVGEYCLMCGGGGKHPPSFSPILKSN
                     AGWAEIVQLPYNGTPQYGLLQADRNRFYAYTNPDRPNEFFLLENRNNTGYEGPYGGAG
                     PCAPGRGLVVWHIDRNGSNMYSSIQDPMVEEEELFRTPYYAFVVEAEPGSEDPWYADP
                     NPRPDANDTFYAEHGANPLYSYPPLPNSLMFGETDPNDPDKVDLSHPDLCFWDREDFS
                     KGRTVISKMVLSTFGEQGHYMPFIAGVNPSQSADWMILGVMGQPKITTTCSTIRARCN
                     WGQNPPNQHFRVFNSGGQGTLYFTVTSDQPWLLVSPGSGYAQPKLGGENFQISFDATN
                     LAPGTHTGTVTVFAPDQVPATPLHISVTLTVEARPELLVTPGAMSLTLKPNEVSTDRL
                     VKVTNMGGSSMKYTVTSSCAGSPWNWLTPYVTSGLCVGGMQDSFPLTIEPSDASQKPP
                     VLPAGIHTGIVTVASPGALNSPQSTTVTLNVDGYIEVVSPNGGEVWQPNLDLTVPIVW
                     ATNSAEPVGIELVRWDSGAMNYVVVETIASGIPHDGDPDADGRSMYNWPLPIDFTPGA
                     DYRVRVFSGGSSWSTAAHRDESDADFRIMRVVYNTTMDTNPGWTLGGGWAWGKPLGKG
                     GGEGGSGNVSNPDPTSGYTGESVLGYNLSGNYANGMTTTEYATLGPINCEDFVDLSLT
                     FRRWLGVERTDNAMIEISSNNTTWHQVWHNPPDVDITDGDAGTWALVSYDISDYADSK
                     PTVWIRWGLGPTDATDRFCGWNLDDVAVHGRYMRVGAITALITPPGAASAGAQWRRLG
                     TTTWFNSGDTETGVPVGTYAIEFKAVPTWLAPENVIVEVLKSKTAQVNGTYRQIGSLQ
                     VNIAPAGAASAGAQWRRVGQTTWRNSGFIESGIFTGSYTVEFKPINRWRAPASQPVTI
                     ASEQTTLLTGTYEEIKYGSLQVLISPAEAVSKGAKWRRAGTGAWLNSGATESDLPVGP
                     YSIEFKAVADWQSPPAQSVAVTENQVAVATAAYIKIIKQGSLQVTIQPVGARMAGAQW
                     RRVGTLAWHNSGVVEAGVDEGEHLVEFKPVPGWVEPADAFVIVAPEQTA"
BASE COUNT          863 a         1181 c         1310 g          781 t
ORIGIN      
        1 tgataatggc gccaggggcc tgggccgtgc cgtatttcgg cgacgaggtg tttacgtatc
       61 gtcagccgga cggttcgacc ttcgaagtga ggctgtacgg cgacgaattt tttgcctacc
      121 agcgtacagc ggatgaaggg cgcgaagtgg ttctggatga agccacgggg ttctggtgtt
      181 atgccaggct ttcagccgat gggcgttcct tcgtctcaac aggcgttccg gtgaccacgc
      241 ttgcaaaatc ggcgccagaa accaacaagg cgctggcgat ctcggccgga gcggcgcaac
      301 ccaatgcgac gctgccttat gatgtcatca tatcgcgtgt gaaagccgag cgggaggcat
      361 tgaccaacgc cgcccgggac tatctggcgg gcaccggaat cccgtatgaa gtccagccaa
      421 aagcggcgac aggtacgatc attggcctgt gtattctggt ggatttcccc gatgacgtag
      481 cgaccatatc tcaggcgcaa gtggagcggt ttctgaacca gccctcagac tacaccgaat
      541 ttggtaacgc gtgctcggtg aacgagtatt tccacattca atcgaatggc ttgctcaact
      601 atgtcaatgt cgttaccccc tggattcgga tgccgcatcc gaaaatctat tacgacgaca
      661 attcgattaa cagctcgaaa ccgggggaac tcctttcgga ggcccggagc attctgtcgc
      721 aacagggcta cgatgttaac gcgtatcccg cggtgaatgt cttttatgcg ggtaactgcc
      781 tgtccggctg gaggcgcggt ctgtggccgc actcggcggg caattatcag atgaccgcta
      841 tgggcggcga gttggcgctg ggcacattct gccacgaaaa tggccacatg atctgcgggt
      901 tcccggacct ctattcgtat acagccgcat atctgggcgt ttccgggtca ctcgtcggcg
      961 aatattgcct gatgtgtggt gggggaggaa aacacccacc cagtttttca cccattttga
     1021 aatccaatgc agggtgggca gagatcgtgc agttgccgta taacggcacc ccgcagtatg
     1081 ggctgttgca ggccgaccgg aaccgcttct acgcctacac gaacccggac cggccaaatg
     1141 aattcttcct cctggagaac cggaacaaca ccggatatga gggcccgtat gggggggccg
     1201 gtccctgcgc gcccggccgc ggcctggtgg tctggcatat cgatcgaaac ggcagcaata
     1261 tgtacagctc gatccaggat ccgatggtcg aggaagagga attgttcaga acgccatatt
     1321 acgcgttcgt tgtggaagca gagcctggct ctgaagaccc gtggtatgcg gatcccaacc
     1381 cccggccgga tgcgaacgat acgttttacg cggagcacgg agcgaatccg ctgtattcct
     1441 acccgcctct gcccaacagc ctcatgtttg gggaaacaga ccccaatgat ccggacaagg
     1501 tcgatttgag tcaccccgat ctgtgcttct gggaccggga ggatttttcg aaaggccgga
     1561 cggtcatttc gaaaatggtg ctcagcacct ttggcgagca gggccattac atgcccttca
     1621 ttgccggtgt caacccctcc cagtcagcgg actggatgat actgggggtc atgggtcagc
     1681 ccaagattac aacgacctgt tcgaccatac gggcgcggtg caactggggc cagaacccgc
     1741 cgaaccagca tttccgggta ttcaattcag gcggccaggg cacactctat ttcacggtta
     1801 cgagcgatca gccctggctg ctcgtctccc ccgggtcggg ctacgcccag ccgaagctgg
     1861 gaggggaaaa tttccagatt tcctttgatg ccaccaacct ggcgccgggc acgcatacgg
     1921 gaacggtcac ggtgtttgcg cccgaccagg tgccggccac cccgctgcac attagcgtaa
     1981 ccctgaccgt tgaggcgcgt ccggaattgc tcgtcacgcc gggcgccatg tcgctgaccc
     2041 tgaagccaaa cgaggtatcc accgaccgcc tggtcaaggt gacgaacatg ggcggcagct
     2101 ccatgaaata taccgtgacg agcagctgcg caggctcgcc ctggaattgg cttaccccct
     2161 acgtcacatc cgggctgtgt gtaggaggca tgcaggacag ctttcctctc accatcgaac
     2221 ccagcgacgc cagccagaaa ccgccggtgt tgcctgcggg tattcacacg ggaatcgtga
     2281 cggtcgcctc accgggggcc ttgaattcgc cgcagagcac aaccgtgacc ttgaatgtcg
     2341 acggctacat cgaggtggtg tctcccaatg gcggggaagt ctggcagcca aacctggatc
     2401 tgaccgttcc gatcgtctgg gcgaccaata gcgccgagcc ggtcggcatt gagcttgtcc
     2461 gctgggattc aggtgcgatg aattatgtgg ttgttgaaac gatcgccagc ggcatcccac
     2521 atgacggaga ccccgacgcg gacggccgga gtatgtacaa ctggccgttg ccgatcgatt
     2581 tcacgccagg cgcagactat cgggtccgcg tgttcagcgg cggatcgagc tggagcacag
     2641 cggcgcatcg cgatgagagc gacgccgatt tccgcatcat gcgcgtggtg tacaacacca
     2701 ccatggacac caatcccggc tggaccctgg gaggcggatg ggcgtggggc aagccgttgg
     2761 gcaaaggcgg aggagaaggc ggctcgggca acgtcagcaa tccggatccg acgtccggct
     2821 acacgggcga atcggtgctc ggctacaacc tgagcggcaa ctacgccaac gggatgacca
     2881 cgacggaata tgcgacgctt ggtccgatca attgtgaaga tttcgtcgat ttgagcctga
     2941 cgtttcggcg ctggctgggt gtcgaacgga cggacaacgc gatgatcgag atcagcagca
     3001 acaatactac ctggcaccag gtatggcata acccgccgga cgtggacatt acggatggag
     3061 acgcgggaac ctgggcactg gtgtcctacg atatttccga ctatgccgac agcaagccaa
     3121 ccgtatggat acgctggggc ctcggcccga cggatgcgac ggaccggttc tgcgggtgga
     3181 atctggacga tgtggcggta cacggccgct acatgcgggt cggcgcgatc acagcgctga
     3241 ttacgcctcc gggcgcggcc tcggcggggg cacagtggcg ccggctgggt acaacgacct
     3301 ggttcaacag cggcgacacg gaaacgggcg tgccagtagg cacctatgcg attgagttca
     3361 aggcggtgcc gacctggctg gcgcctgaga acgtgattgt agaggtgctg aaatcgaaga
     3421 ctgctcaggt aaacggtacg tatcgccaga taggttcgct gcaggtgaat atcgcaccgg
     3481 ccggcgcggc ctccgcgggc gcacaatggc gccgggtggg tcaaaccacg tggcggaaca
     3541 gcgggtttat cgaaagcggc atatttaccg ggtcgtacac agtagaattc aagcccatca
     3601 accggtggcg cgcgccggca agccagccgg tgaccatcgc gagcgagcag actacgctgc
     3661 tcacgggcac ctatgaagag atcaagtacg gttcgctgca agtgttgatc agcccggcgg
     3721 aagcggtgtc gaaaggcgcg aaatggcgcc gtgccggcac aggagcctgg ctgaacagtg
     3781 gcgccacgga aagcgacttg cctgtcgggc cgtattcgat cgagtttaag gcggtcgcgg
     3841 attggcagtc cccgcccgct cagagtgtgg ccgtcaccga gaatcaggtg gcggtcgcca
     3901 ctgctgcgta catcaagatc atcaagcagg gttcgctgca agtcaccatt cagccggtcg
     3961 gcgcccggat ggccggagca cagtggcgcc gggtgggcac gctcgcgtgg cacaacagcg
     4021 gagtagtcga agcgggtgtg gatgaaggcg agcacctggt tgaattcaaa ccggtgcccg
     4081 gctgggtaga acctgccgat gccttcgtga tcgttgcgcc ggagcaaacg gccaa
//
LOCUS       DAOPBQ010000334         4125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17715_length_4125_cov_2.981081, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000334 DAOPBQ010000000
VERSION     DAOPBQ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17715_length_4125_cov_2.981081"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1285
                     /locus_tag="PKW60_10675"
     CDS             26..1285
                     /locus_tag="PKW60_10675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPJ99940.1"
                     /translation="MVLGAAFVLSYGAALRIIGPVSDLQSGLTRIGGGELAYRLSIPP
                     VPHLAELARSINQTADLLQKDIQALNEERNLRTLILANMTRGVIAIDNRHAVLDMNDS
                     AKKLLNLSHPTPEGTLIGEVVRYPELLSLIEESERCGEPVAKEMMAEGASDVRLEMRA
                     SALKDVAGRRIGTLLVLADVTLLRRLETIRQDFVANVSHELRTPVTAVKGFAETLLDG
                     AVKDPEKAERFLKIIVRQAGQLESTIRDLLELSRLEDHSSQTLECQKTPVIEVLTNAV
                     ELCQGCADKRGVTLSLSCEEGLSAMVHAGLMEQALVNLIDNAITYGAVGAQKANVDIE
                     AASEGSTVRVSVRDYGPGIERQHLDRLFERFYRVDRGRSRELGGTGLGLAIVKHIVRI
                     HNGVVSVESEVGKGSVFTIRLPAPSIT"
     gene            complement(1549..2958)
                     /locus_tag="PKW60_10680"
     CDS             complement(1549..2958)
                     /locus_tag="PKW60_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPJ99941.1"
                     /translation="MILAAHVFAADPEFAIGYEYPAAHGKVPTTETFHRIIDIISDLG
                     YNQFHIYLKNTFAYTNHPAALKRNPAPVTAEEIRALDDYCAKKGIELVPYQASFGHME
                     PWLAMPEYNHLAETPKGVRTEKPKLNCGPISLCATDPASISFLAGLYDELLPNFRTKK
                     INVGCDEVWDLRDPDCRSAAEVKRLGAGRVYLNCILKIHENIQARGHVMMCWADMIFL
                     HPELVPDMPKKNIIALDWGYEANTPFERHGELLKKAGVPFYVCPGTSGWGSLFGRVDN
                     MKKNIDNAMAAAKKHGASGLLLTDWGDGKHPQPWIAGLPGIIYAAARAKGEEMDDAKL
                     AAAIDRVTRCTCGEALIRYGNLYLKCGVIRSNGTALYDIFQKASGFKRPATMTEEKMA
                     DVFAEWRAAKAVLNLSGAPQWVKDDFELLDVLATVVELRWKGQHDAVKTLAPRYRELW
                     VKQNRPGNIDSDTKHLFRR"
     gene            complement(3004..>4125)
                     /locus_tag="PKW60_10685"
     CDS             complement(3004..>4125)
                     /locus_tag="PKW60_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99942.1"
                     /translation="KLVLCTGPMGVAGSTRMQATTIELLVAGMAFEAGLNEHLQARLT
                     PAQIASLGLDARTPEKTLQQFETLLGQIRNTGNVAALARLADYEEGLYARGGRITYFA
                     DGYLLDIFTDTTERAPTFKIPPFRRCDDTLSPAPWAFVKDPLRTTPEAWLRVLEHEPR
                     CLDWTPDTYIRLGASQNIRANPPQIDRVTLHKFIIGKEPDSSRTEAKPNAAMAVLIGS
                     EAALLQADTPSAWFGAYQAASEKFDARSALVIGRQTPAGWDDTLINVDVDIPDTPLNL
                     FSHLAVKLVLNNVSTATMGKMGRLNSNWMAHVEASNKKLIDRSTRLIVELAGVDYETA
                     CIALFETLEEMKGWAEARRKTTSPAAHTVEKLQKKNPLR"
BASE COUNT          708 a         1170 c         1352 g          895 t
ORIGIN      
        1 ccgggtgttg atcgtcttgc tgctggtggt gcttggagcc gcatttgtgc tgagttatgg
       61 tgccgcgttg cgcatcatcg gccctgtctc cgatctgcag agtggactga cgcggatcgg
      121 cggcggcgaa ctcgcctatc ggttgtcgat cccgcctgtg ccgcatctgg cggaacttgc
      181 gcggtcgatc aaccagacgg cggatttgtt gcagaaggat attcaggcgc tgaacgagga
      241 gcgtaatctg cggacactga ttctcgccaa catgacgcgc ggtgtgatcg cgatcgataa
      301 ccggcacgcg gttttggata tgaacgattc ggcaaagaag ctgctgaacc tcagtcaccc
      361 gacgccggaa gggacgctga tcggcgaggt cgtccggtat ccagagttgc tgtcgctgat
      421 cgaagaaagc gagcggtgcg gggaaccggt cgcaaaagaa atgatggcag aaggcgccag
      481 cgatgtgcgg ttggaaatgc gggcctctgc gctgaaggat gttgcggggc gccggatcgg
      541 aacgttgctg gttttggctg acgtgacctt gttgcggcgg ttggagacga tacggcagga
      601 ttttgtcgca aacgtgtctc acgagctgcg cacgccagtc acggcggtca agggatttgc
      661 cgagacgctt cttgacgggg cggttaaaga tcccgaaaag gcggagcggt ttctcaaaat
      721 tattgtgagg caggcggggc aactggaatc gaccatccgc gacttgcttg agctgtcccg
      781 gctggaggat cactcgtctc aaacgcttga gtgtcaaaag acgccggtca ttgaagtttt
      841 gacgaacgcg gtggagcttt gtcagggatg tgcggacaag cggggcgtca cgctttcgct
      901 ttcgtgtgag gaagggttga gcgcgatggt tcatgcgggg ctgatggaac aggcgcttgt
      961 gaacctgatc gacaatgcca tcacatacgg tgcggttggg gcgcagaaag ccaacgtcga
     1021 tattgaggcc gcgagcgagg gctcgacggt gcgtgtttct gtgcgcgatt acggccccgg
     1081 gattgagcgc caacatctcg accggttgtt cgagcggttc tacagagtgg acagggggcg
     1141 cagccgcgag ttgggcggca cggggttggg gctggcgatc gtcaagcata tcgtccgcat
     1201 tcacaacggg gttgtgagcg ttgagagcga ggtcggcaag ggctcggttt tcaccatccg
     1261 tttgcccgcc ccgtccataa cttgaacgtt gggcgttcag cacctcaact cacaattaag
     1321 aagttgtcct cttcgtttat ttccgctctg cgggcttggc gcaagttcaa tggccgtctt
     1381 cacgaaatcc ggctcgtatt cgaacacata aaaacgcaag ttcggatcca gcgccaccgc
     1441 gccgactttc tgctgcacga gcttgttgtg ccgtccgtga ccttcccatg ggatgctcgt
     1501 ggttcaattc ctgttgagca tgccgccggc acccccttct cccttccctc agcgtcggaa
     1561 gaggtgtttc gtatcagagt cgatattgcc gggccggttc tgcttcaccc aaagctcgcg
     1621 gtaacgcggg gcgagggtct tcacggcatc gtgttgaccc ttccagcgga gctcgacgac
     1681 ggtcgcgagc acgtccaaga gttcgaagtc gtccttcacc cactgcggcg cgccgctgag
     1741 gttgagcact gccttggccg cgcgccattc ggcgaacacg tccgccatct tctcctcggt
     1801 catcgttgcg gggcgcttga agccggaggc cttctggaag atgtcgtaga gcgccgtgcc
     1861 gttactgcgg atgaccccgc acttgaggta gaggttccca tagcggatca gcgcctcgcc
     1921 gcaggtgcag cgcgtgaccc ggtcgatcgc cgcggcgagt ttcgcgtcgt ccatctcctc
     1981 gcccttcgcg cgggcggccg cgtaaatgat tccgggcagc cccgcgatcc agggctgcgg
     2041 atgtttgccg tcaccccagt ccgtaaggag cagacccgag gcgccatgct tcttcgcggc
     2101 ggccatggcg ttgtcgatgt tcttcttcat gttgtcgacg cgcccgaaga ggctgcccca
     2161 gccgctcgtt cccggacaga cgtagaaggg aacgcccgcc tttttgagca actcgccatg
     2221 tctttcaaag ggagtgttgg cctcgtagcc ccagtccagc gcaatgatgt ttttcttcgg
     2281 catgtccggc acgagttcgg gatgcaggaa gatcatgtcc gcccagcaca tcatcacatg
     2341 cccgcgcgcc tggatgttct catggatttt gaggatgcag ttcagataca cgcgtccggc
     2401 acccaaccgc ttcacctcgg ccgcgctgcg gcagtcggga tcgcggagat cccagacctc
     2461 gtcgcagccg acgttgattt tcttcgtgcg gaaattcggc aggagttcgt cgtagagccc
     2521 ggcgaggaag gagatactcg ccggatctgt ggcgcagagc gagatcgggc cgcaattgag
     2581 cttcggtttt tcggtgcgaa cccctttcgg cgtctcggcg aggtggttgt attccggcat
     2641 cgcgagccaa ggctccatgt gcccgaacga ggcctgatag ggcacgagct cgattccctt
     2701 tttcgcgcag tagtcatcga gcgcccggat ctcctccgcc gtgaccgggg cagggttccg
     2761 tttcagtgcg gccggatggt tcgtgtaggc gaaggtgttc ttcaggtaga tatgaaactg
     2821 gttgtacccg agatccgaga tgatgtcgat gatccggtgg aacgtttctg ttgtcggcac
     2881 cttcccgtgc gcggcgggat attcgtaccc gatggcgaat tccggatcag ccgcgaaaac
     2941 gtgggccgcg aggatcaatg cgaatacgga tgcaatgctg tttttcatgg ggatattcct
     3001 ttcttacctg agagggttct ttttctgcag tttctctacc gtgtgcgccg caggcgaggt
     3061 cgttttgcgg cgggcttcgg cccagccttt catctcctcc agtgtctcga agagggcgat
     3121 gcaggcggtc tcgtagtcca cccccgcaag ctccacaatc aggcgggtgc tgcggtcgat
     3181 gagcttcttg ttggacgcct caacgtgcgc catccagttg ctgttcagcc ggcccatctt
     3241 gcccatcgtt gcggtcgaga cattgttcag gaccagtttc acggccagat gcgaaaagag
     3301 gttcagcggc gtgtcgggga tgtcgacgtc cacgttgatc agcgtgtcgt cccagccagc
     3361 gggcgtctgg cgcccgatga ccagcgcgga gcgggcgtcg aatttttccg aagcggcttg
     3421 atatgcgccg aaccaggcgg agggagtgtc ggcctgcagc agcgccgctt cgctgccgat
     3481 cagcacggcc atggcggcgt tgggcttggc ttcggtccgt gagctgtccg gctcttttcc
     3541 gattatgaat ttgtggagcg tcacgcggtc gatctgcggc ggattggcgc ggatgttctg
     3601 ggaagcgccc agccggatat aggtgtcggg cgtccagtcc agacagcgcg gctcgtgctc
     3661 cagcacgcgc agccaagcct ccggggttgt ccgcaacgga tccttcacaa aagcccacgg
     3721 cgcgggcgac agggtgtcgt cgcagcggcg gaagggcggg attttgaagg tcggcgcgcg
     3781 ctcggtggtg tcggtgaaaa tgtcgagcag gtagccgtcc gcgaaatagg tgatgcggcc
     3841 gccgcgcgcg tacagacctt cctcgtaatc cgccagccgc gcgagcgcgg ccacgtttcc
     3901 ggtgtttcgg atctgcccca gcagtgtttc aaactgctgc agtgtctttt cgggcgtgcg
     3961 cgcatcgagc ccgagagagg cgatttgcgc gggcgtgagt ctggcttgca gatgctcgtt
     4021 caggccggcc tcaaaggcca tgcccgcgac cagcagttcg atggtggtgg cctgcatccg
     4081 cgtggagccc gccacgccca tgggcccggt gcacagcacg agctt
//
LOCUS       DAOPBQ010000335         4124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17721_length_4124_cov_2.943229, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000335 DAOPBQ010000000
VERSION     DAOPBQ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17721_length_4124_cov_2.943229"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="PKW60_10690"
     CDS             complement(<1..1202)
                     /locus_tag="PKW60_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99943.1"
                     /translation="MMSQGLSDTFVTDTRGIVRQIVQPIHLPEWENLSDAQSAAPYEL
                     PQVREQLERWNGSTFVPAETMGSGARIYVIAQGWNTVHGLPNAGLDWIEEMARALKQQ
                     DSGSLILRWDWEYEAATVLPPTAEAATQGMELAAALQNAGVFQASQVHFIGFILGASV
                     CAHAAQSLYLGTPPPAFEARLTLLDPPESLLAATTALFPKNGRARLQDAVAFLHNQGV
                     QVDCYATKFGVPYDHGHSFYLNGIANVRDTPSAIPMADEYSDHAVAPWWFIASIQPGT
                     TTELPQTSLADYFHYLENDPDYFWDSVGSNGFNFKFSAVNPSAVWEPQQVAWAYIGLA
                     FNTDPFQLWFGALSPFRYYRSHGFSADGTVLYIPRHQQEINRRQPLHPLTRTEGICGY
                     QPYKFAQH"
     gene            complement(1358..2458)
                     /locus_tag="PKW60_10695"
     CDS             complement(1358..2458)
                     /locus_tag="PKW60_10695"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HPJ99944.1"
                     /translation="MRRMHLFFAVGLVWVVMTGAAADPVVSDVTAAQRTDGSGLVDVY
                     YTLTGTTPTIRIDLAFSNDGGVTWDIIPSANTVTGDVGDGISNGSDRHIVWDAARDRP
                     DVYWPDTRARVRAVDIGQSTTFMLPGGVKLDMVRIPAGTFQMGAPQGLDPYSHSEQRP
                     VHTVTIGEDFFMGRYEVTYRQWYAVMKDYFAWQTAWDTPEGPDYPVAWVMWDDAQEFI
                     ARLNTMGIGTFRLPTEAEWEYACRAGSGTLWHFGDDEAMLTHYAWYMDNNATGGTKPV
                     GQKLPNPLGLYDMHGNVIEWVQDMWHEDYTGAPTDGSAWEEEPANPTYVSRVTRGGSY
                     SDFPHFCRSAFRLGFTHYYRGSICGFRVVRTQ"
     gene            2795..>4124
                     /locus_tag="PKW60_10700"
     CDS             2795..>4124
                     /locus_tag="PKW60_10700"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPJ99945.1"
                     /translation="MPGVSLWILSGVAMMLSSAATGEFGEAKWLRDPVFKGVEIIDLY
                     HKEHAEKPDLSGPRNVHTLLRKQVTLPEAPTAARLVITGDDYYTFHINDAFVVQGPEP
                     GYPFAHPYYKLDVTGALQAGDNTLAAHVFYQGLLNRVWNSADNRSGFMMALTVTYAGG
                     SSETFVTDESWKLHTLAAFPTGRTLGYDTQFAEDIDMRLIPAGWRANGFDDSRWKAPL
                     CGRQDHVFVEQMTPPLEHWRADPQVVEKKGKGHYFYDFGQEIVGHTRIRLQGPKGHKI
                     EVRHGEELSEPGTVRYDMRAKCVYQEFPILSGRRETIEFFDYRAFRYMEILNAPEEPE
                     VWVDVRHHPFDPEAATFKASDELLTAIWQLCKNGVQFGAQGGFLDCPSREKGQYLGDA
                     LITGHSHVVLTGDGSLLAKSIQDFAHSRRICPGLMAVAPGSLMQEIAEYSL"
BASE COUNT          856 a         1171 c         1219 g          878 t
ORIGIN      
        1 atgtgctgcg cgaatttgta gggctgatag ccgcaaatac cctccgtgcg agtcaatggg
       61 tgaagtggct ggcgccggtt gatttcctgc tggtggcgtg gaatgtagag aacggttcca
      121 tccgcagaaa aaccgtggct gcggtagtac ctgaaaggac tgagggcgcc aaaccacaat
      181 tggaatgggt ccgtgttgaa cgcaagcccg atgtaggccc acgccacttg ttgcggctcc
      241 cagaccgcgg acgggttgac ggcgctgaat ttgaagttga aaccgttgct gcccacactg
      301 tcccagaaat agtccgggtc attttccaga tagtgaaagt aatccgccag agaagtctgc
      361 ggcagctctg ttgttgtacc tggctgaatc gaggcgatga accaccaggg ggccacggcg
      421 tgatcgctgt actcgtccgc catcggtatg gcgctggggg tgtcccgcac attcgcgatg
      481 ccgttcaaat agaacgaatg gccatggtca tagggcactc cgaacttcgt ggcatagcag
      541 tccacttgta cgccctggtt gtgcagaaaa gcaaccgcat cctggaggcg tgcgcggcca
      601 tttttcggga ataacgcggt tgtggcggcg agcagactct ccggcgggtc aaggagggtc
      661 agacgagcct cgaacgcagg gggaggtgtt ccgagataga gcgactgcgc cgcatgcgca
      721 catacggaag ctccaaggat gaaaccgatg aaatgtacct gagatgcttg aaaaacgcct
      781 gcattttgga gcgcagccgc caattccatc ccctgtgttg cggcttcggc ggtgggcggc
      841 aagaccgtgg cggcctcata ttcccaatcc caccgaagga tcaggctgcc ggaatcctgt
      901 tgcttcaggg cacgcgccat ttcctctatc caatcgaggc ccgcattggg cagcccgtgc
      961 acggtattcc agccctgggc aatcacgtag atccgggcgc cggatcccat ggtctctgcc
     1021 ggtacaaacg ttgagccgtt ccatcgttca agctgttcgc gcacctgcgg gagctcatac
     1081 ggcgctgccg actgtgcgtc gctcaggttc tcccattcgg gcaaatgaat gggctggact
     1141 atttgccgga cgatgccccg cgtatccgtt acaaacgtgt ctgacaggcc ctggctcatc
     1201 attacgccct gcttggtgcg tggtggagat gccggtgaga cggcgggaga tgctgccatc
     1261 accgcactgg agacgcacgc caaaacggca acaatatgta tcatgcggtt cgctgagatt
     1321 gtcatcaccg tgcctccgcc tggcttgatc ttttccatta ttgcgtcctc acaacgcgga
     1381 acccacaaat tgagccgcgg taatagtggg tgaagccgag ccgaaaggcc gaacggcaga
     1441 aatggggaaa gtctgagtac gaaccgcccc gagtcacccg agacacatag gtcgggtttg
     1501 ccggttcttc ttcccaggcg cttccatccg tgggtgctcc cgtatagtct tcgtgccaca
     1561 tatcctgcac ccattcgatg acatttccgt gcatatcgta gagccccaat ggattgggga
     1621 gcttctggcc cacgggtttg gtgccgcctg tcgcgttgtt gtccatgtac caggcgtaat
     1681 gggtgagcat tgcttcgtca tcaccgaaat gccacagagt accggagcct gcgcggcacg
     1741 cgtattccca ctcggcctct gtaggcaagc gaaaagtccc gatacccatg gtgttcaggc
     1801 gtgcaataaa ttcttgcgca tcgtcccaca tcacccacgc aacgggataa tccggaccct
     1861 caggtgtatc ccaagctgtc tgccaggcga aataatcttt catcacggca taccactgtc
     1921 tataggtgac ttcgtaacgg cccatgaaga agtcttcccc gatggtcacg gtgtgaaccg
     1981 gcctctgttc ggaatgactg taagggtcga ggccctgggg ggcgcccatc tggaaggtgc
     2041 ctgctggaat ccgcaccatg tcaagcttta ccccaccggg aagcatgaat gtagtcgatt
     2101 ggccgatgtc cactgcgcgc acacgcgcgc gggtatcggg ccagtagacg tcggggcggt
     2161 cccgggcggc atcccacacg atgtggcggt ccgaaccgtt cgaaatgcca tcgccgacgt
     2221 cacccgtgac cgtgttcgcc gacgggatga tgtcccaggt gaccccacca tcgtttgaaa
     2281 aggcgaggtc aatccggata gttggcgtag tcccggtcaa cgtgtagtag acatcgacca
     2341 gtcccgaacc gtccgtccgt tgggctgccg tcacatcgga gacgaccgga tccgccgccg
     2401 cgccggtcat caccacccac actagaccca ccgcgaaaaa caaatgcata cgcctcatgg
     2461 cccgactcct gtgcgcaaag gacttgcctt gctccttttg cgaagatatg aagccgtaat
     2521 tgctgctttt atccgtcatc ttctaccgct gcattcacat gaataaattt acttcgcgag
     2581 gtggtttttg tcaagagttt tcatgcatat ggtgcttata tgaaatccgt acgcaacatg
     2641 tcgtggtatc aaggcggtct atattgtctg ttaagcatat atctctttat ttaatagaag
     2701 tatggattac ccttcctggc ttcctcattc ctctttcgcg ccggctcgtg gtaccatgcg
     2761 gcgcagttga gccaagcgta tgggaggatt gcaggtgccg ggcgtttcat tgtggatttt
     2821 gagcggggtg gccatgatgc tatcgagtgc ggcgacgggc gagtttgggg aggccaaatg
     2881 gctgcgggat cccgtgttca agggcgttga gatcatcgac ctttatcaca aggaacacgc
     2941 cgagaaaccg gacctgtccg ggccgcggaa tgtgcacacg ctgctgcgga aacaggtcac
     3001 gctgcctgag gcgccgactg cggcgcgatt ggtgatcacg ggcgacgatt actacacatt
     3061 tcacataaac gacgccttcg tggtgcaggg tcccgaaccg gggtacccat ttgcccatcc
     3121 ctactacaaa ctcgacgtga cgggggcgct tcaggcgggc gacaacacgc ttgcggccca
     3181 cgtcttctac caggggcttc tgaaccgggt ctggaacagc gcggacaacc gatcgggatt
     3241 catgatggcg ctgaccgtta cctacgctgg cggcagcagc gagaccttcg tgaccgacga
     3301 gtcgtggaaa ttgcacacgc tggccgcgtt tcccaccggg cgtaccctgg gatacgatac
     3361 ccagttcgcc gaagatatcg atatgcggtt aatcccggca ggctggcgcg ccaatggttt
     3421 tgatgattcg cgctggaaag cacctctctg cggccggcaa gaccatgtat ttgtggagca
     3481 gatgacgccc ccgctcgagc attggcgcgc agacccgcag gtggtcgaaa aaaagggaaa
     3541 aggacactat ttctacgatt ttggacagga gattgtcggt catacccgga tccgcctgca
     3601 ggggccgaaa gggcacaaga ttgaagtgcg ccacggcgaa gagctgtcag aaccggggac
     3661 ggtccgctac gacatgcgcg cgaaatgtgt gtaccaggaa tttccgattc tgtccggccg
     3721 cagagagacc atcgagtttt ttgattaccg cgcgttccgc tacatggaaa tacttaatgc
     3781 accggaggaa ccggaggtgt gggtcgacgt gcggcaccat ccctttgatc ccgaagcggc
     3841 tacattcaag gcctccgacg agctgctgac tgcgatctgg caactctgca aaaacggggt
     3901 gcagtttggc gcccagggcg gctttctcga ttgcccctcg cgcgagaaag gtcaatatct
     3961 gggcgatgcc ctgattaccg gccattccca cgtggtgctg accggcgacg ggtcgcttct
     4021 cgcgaaatcc atccaggatt tcgcgcattc ccggcggatc tgccccggat tgatggcggt
     4081 tgcaccgggc agcctgatgc aggagatcgc cgagtattcg ctgc
//
LOCUS       DAOPBQ010000336         4123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17727_length_4123_cov_3.720993, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000336 DAOPBQ010000000
VERSION     DAOPBQ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17727_length_4123_cov_3.720993"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="PKW60_10705"
     CDS             complement(<1..1170)
                     /locus_tag="PKW60_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99946.1"
                     /translation="MRMCVAVLSLAVVVAMSMAAPAQEDPGPEVLREQIQCYPYTVRI
                     FGPSELMWKSRIEFSKDGAVVFEHSLDENVAVLTRCATVGDVDGDGRGELILESYSGG
                     AHCCFSYLIFSLGDTPALMARFEAQDSGAMLKDDDGDGIIEFHSEDMAFAYFGAPFAA
                     SVRAPIVVVYDNGAFHLAEHFMRKPAPSEDEWKKRADGVRADWDNKEVMDMTFDSEEW
                     GRVPPSLWRNMLDLIYSGNGELAWKFLDELWPGDKPGKDAFRKDFEAQLATSAYYDDV
                     LKLNGWAPAEEEQSGALRTMRYTPGSAEEAVAWQGELRARLCELLHVSDLMETGASVP
                     LGARVLATEEREGFRLDTLELRATPGRVFQAMLAVPAEASRPMPAEISSGVSASVS"
     gene            complement(1214..>4123)
                     /locus_tag="PKW60_10710"
     CDS             complement(1214..>4123)
                     /locus_tag="PKW60_10710"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HPJ99947.1"
                     /translation="RKWQDDTISGKILDGAIREVDGNLGDWERVPNAYLINTPEQVVW
                     GANSWTDPADLHGTVRLAWRQEYLFIAAEVVDDTLHQTQRGANIWQGDHVEIYLDAAP
                     DIDPGRETFGPEQYHLALSPGNFQNTGDPFTDCPPEAYCYRPEAQPIEGARIASVRTA
                     SGYQLEAAIPWAFFNITPETGTFLRIEVTLSDTDGPEARQETMLTLSSAEWGHRRSRL
                     IPAVLSGTDGVPPERANREPLFDEVRLERDETETLTITAPPAPEGKLAVLFLKARLES
                     DRVAGHTAALRVFLNGQPLSGERLLEKPQRVLSRNGTVYSMYAGDRLTTYYAPDFEKA
                     DRDTYYGLMEGVKACEFQLDVTDLLREGENALTLEHAAASTVDRALVVGEGELVFRSP
                     PPPPKPKAGPPTGPLEDCAPLPTHETAFTYRELEDARIEVTVAGETFVVESRFSCPEP
                     AWKTGSCDYFRHSRQITRLPEGLEVADTFTNLTDGNLALMHRHEVQMDGLQAVWLSGL
                     PQPGLSGKSASSQNPTTFGTTRRTGIGLLPREDVFRIHVANYALNDSLGLADNNLVLP
                     PGKEYCARWLIVPSEAPDYWRFINAARRLMDANFCIDGGFAFLRSDSITDKWSDELLS
                     NFLTFKDPRYVCASIGHLTVNGEPCHGTAFQQVPHDSFINSFARFRRLYPEASYLVYF
                     HCFLDVTEEGPQRFADARILGADGSQADYGRPNLRLYLPTESNSYGPAVEKNVDIILD
                     EIHADGVYWDEHAYSRVMYHFGEPWDGVSGDIDPQTMDIVRLKSSVTLLSESWRLALA
                     KRILARAPLIGNGAPFTQAMVGLHFPCFVETGSITNCTHNHLYSPVALGDHLTERSEL
                     DAYHVMLAALDYGCVYHWYNDVLVIPTHHHLTRYMFPITPLELHEGYIIGEERIVTNR
                     SGLFGWGDSSRHEVHVFNNEGREVEDFEAPWITRDGKTYTELRIAEDWSAAIIRK"
BASE COUNT          780 a         1300 c         1252 g          791 t
ORIGIN      
        1 cgagacgctt gcgctcaccc cgctgctgat ctccgcgggc atcggcctgc tggcctccgc
       61 cggaaccgcc agcatggctt gaaacacacg tcctggcgta gcccgcagtt cgagcgtgtc
      121 gagccggaac ccttcgcgct cttcggtagc cagcacgcgg gcgcccaggg gaactgaggc
      181 gcccgtttcc atcagatcgc tcacgtgcag aagctcacac aggcgcgcac gaagctcgcc
      241 ctgccaggcc accgcttcct ccgccgaacc cggggtgtat cgcatcgtcc gcaaggcacc
      301 ggactgctcc tcctcggccg gtgcccaccc gttcaatttc aggacgtcat cgtaatacgc
      361 gctcgtggcg agctgcgcct caaaatcttt ccggaaggcg tctttacccg gcttatcgcc
      421 cggccagagc tcgtcgagga acttccatgc cagctccccg tttccggagt aaattaagtc
      481 cagcatgttg cgccacagcg agggtggcac acggccccac tcctcgctgt cgaaggtcat
      541 gtccatgacc tccttgttgt cccagtcagc gcgcacgccg tccgcgcgct tcttccattc
      601 gtcctccgat ggcgcaggtt tgcgcatgaa atgctcggca aggtggaagg cgccgttgtc
      661 gtataccacc acgatcggcg cgcgcactga ggccgcgaag ggcgccccaa aatacgcgaa
      721 cgccatatct tcggaatgga actctataat cccgtctccg tcgtcatcct tgagcatggc
      781 gcctgaatcc tgagcctcga agcgagccat cagggcgggc gtatctccaa gcgaaaagat
      841 cagataggag aagcagcagt gcgcgccccc gctgtagctt tccaggatca gctccccccg
      901 gccatcgcca tcgacgtcgc ccaccgtggc gcatcgcgtc agcaccgcca cgttttcatc
      961 cagggaatgc tcgaaaacca ccgcgccgtc tttgctgaac tcaatgcggc tcttccacat
     1021 caactcgctc gggccgaaaa tgcggacggt atacggataa cactggatct gttcccgcaa
     1081 cacctcaggg cccggatcct cctgagcagg tgcggccatt gacatggcca ccacgacggc
     1141 aagactcaag acggcaacgc acatacgcat ttccaaaccc tccctgggca acagcccgca
     1201 atggcccgcc cggtcatttt cggatgatcg cggcagacca gtcttccgcg atgcgaagct
     1261 ctgtataggt ttttccatcc ctggtgatcc agggggcttc gaagtcttcg acttcgcggc
     1321 cttcgttgtt gaacacgtgc acctcgtgcc tggagctgtc gccccatccg aagaggccgc
     1381 ttcggttggt gacgatgcgc tcctcgccga tgatatatcc ctcatgcagc tccagcggcg
     1441 taatggggaa catatagcgg gtgagatgat ggtgcgtggg gatgaccagc acatcgttgt
     1501 accaatgata gacgcacccg taatcaagcg cggcaagcat gacgtggtac gcatccaact
     1561 cgctgcgttc ggtcaggtgg tcgccgagcg cgacaggcga atacaggtga ttgtgggtgc
     1621 agttagtaat gctgccggtt tcgacgaagc acggaaaatg cagacccacc atggcctgcg
     1681 taaacggggc gccgttgccg atcagggggg cgcgggccag gatccgtttg gccagcgcca
     1741 ggcgccacga ctcgctcagc agcgtcaccg acgatttcag ccgcacaata tccatggtct
     1801 gcgggtcgat gtcgcccgag acaccgtccc acggttcgcc gaaatggtac atgacgcggc
     1861 tgtaggcgtg ttcgtcccaa tacacgccgt cagcgtggat ctcgtcgagg ataatgtcga
     1921 cgtttttctc gacggccggg ccgtagctgt tggattcagt gggaaggtaa aggcgcaaat
     1981 tgggcctgcc gtaatcggcc tggctgccat cggcgcccag gatacgggcg tctgcgaaac
     2041 gctgaggccc ctcttcggtg acgtcgagaa aacagtggaa atacacgagg tagctggcct
     2101 caggatagag cctgcgaaac ctcgcgaagg agttgatgaa cgaatcgtgc ggcacctgct
     2161 ggaacgcggt gccatggcat ggttcgccgt tgaccgtcaa gtggccgatg ctggcgcaga
     2221 catagcgcgg gtctttgaaa gtgaggaaat tcgagagcaa ctcatccgac catttgtcgg
     2281 tgatgctgtc cgagcgcaga aatgcgaacc cgccgtcgat gcagaagttg gcgtccatca
     2341 aacggcgcgc ggcattgata aaccgccagt aatccggtgc ttccgacggg acaatcagcc
     2401 accgtgcgca atattctttg cccggcggga gcaccaggtt gttatcagcc aggccgaggc
     2461 tgtcgttcaa ggcgtaattg gctacgtgga tgcgaaacac gtcctcacgc ggcaacaggc
     2521 cgatgccggt ccgccgggtg gttccgaagg tggtggggtt ttgagaggac gcgctcttgc
     2581 cggacagccc cggctgcggc aagccggaca gccagaccgc ctggagcccg tccatctgga
     2641 cttcgtgccg gtgcatcaac gcgaggttgc cgtctgtaag gttggtgaag gtatccgcga
     2701 cctccagccc ttcgggaagg cgcgttatct gccggctgtg gcggaaatag tcgcaggagc
     2761 cggttttcca ggccggttca gggcacgaga agcggctttc cacgacgaat gtttcgcccg
     2821 caaccgttac ttcgatgcgg gcatcctcca gctcacgata cgtgaaggcc gtttcatgcg
     2881 ttgggagtgg ggcacagtcc tccagcggtc ctgtcggcgg tccggccttg ggtttgggcg
     2941 gtggcggcgg gctgcggaac acaagctcgc cttccccgac caccagggcc cgatccacgg
     3001 tcgaagccgc cgcatgttcg agggtcagcg cgttctcgcc ctcgcgcaga agatcggtca
     3061 cgtcaagctg aaattcacac gctttgaccc cttccatcag cccatagtag gtgtcccgat
     3121 ctgctttctc gaagtccggc gcgtagtagg tggttaaccg gtcccccgca tacatggaat
     3181 acaccgtgcc gttacgggaa aggacgcgct gcggcttctc cagcaggcgt tcgccggaca
     3241 gcggctgccc gttcaggaac acccgcagcg cggccgtatg tcccgcgaca cgatcacttt
     3301 caagccgggc tttcaaaaaa aggacggcca gtttgccttc gggcgccggc ggcgcggtga
     3361 tagtcagagt ctctgtctcg tcccgctcca aacgcacttc gtcgaacaag ggctcccggt
     3421 tggcgcgctc aggagggacg ccatccgttc cggaaagcac cgcgggaatc agcctcgaac
     3481 gccggtggcc ccattccgcg ctcgacaggg tcagcatggt ctcctggcgc gcttcaggtc
     3541 cgtccgtgtc agacaacgtc acttcaatgc ggagaaacgt tccggtctcg ggcgtgatat
     3601 tgaaaaaagc ccaggggatg gccgcttcga gctggtagcc gctggcagtg cgcaccgacg
     3661 cgattcgcgc accttcgatc ggctgggcct ccggacggta gcaatacgct tcgggaggac
     3721 agtcggtaaa cgggtcgccg gtgttctgga aattgcccgg actgagcgcc aggtggtact
     3781 gctcgggccc gaaggtctcc cggccggggt cgatatccgg cgcggcgtcg aggtagatct
     3841 caacgtgatc accctgccag atattcgccc cgcgctgggt ctggtggagg gtatcgtcga
     3901 ccacttcggc ggcgataaac aggtattcct gccgccaggc caggcgcacg gttccatgca
     3961 aatccgcggg atccgtccag ctgttcgccc cccagaccac ctgctccggc gtgttgatga
     4021 gatacgcgtt tggaacgcgc tcccagtcgc ccaggtttcc gtcaacctcg cgaatcgcgc
     4081 catctaagat cttaccggag atggtatcgt cctgccattt gcg
//
LOCUS       DAOPBQ010000337         4121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17737_length_4121_cov_3.669454, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000337 DAOPBQ010000000
VERSION     DAOPBQ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17737_length_4121_cov_3.669454"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PKW60_10715"
     CDS             <1..244
                     /locus_tag="PKW60_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPJ99948.1"
                     /translation="RLLAQRQEEEAAERSANRKSQVGSGDRSERIRTYNFPQNRLTDH
                     RIKLTVHRLETIMEGDLQEVIDALIAADRAARIREE"
     gene            244..1092
                     /gene="prmC"
                     /locus_tag="PKW60_10720"
     CDS             244..1092
                     /gene="prmC"
                     /locus_tag="PKW60_10720"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPJ99949.1"
                     /translation="MATIAERLAGAAGLLAAISDTPRLDAELLLAHALGISRAALLAR
                     FQDELNPLPLFDTLLGRRLNYEPLAYILGTWEFFSLDFIVEAPLLVPRPETEHVVEAV
                     LEHIGTRPAAILEIGTGTGCIAVSIAHAARNASIIATDINSHAIAVARKNAERHRVID
                     RLSLRPGNLFEPLQPGEGSFDVICSNPPYIEDTAWPGLDPVIRLHEDPGALLAGLDGL
                     DVIRALVAGAARWLAPGALLAFEIGMGQYERVRDLLARHQYERIRFVRDLAGIERVAV
                     AQKPRS"
     gene            1089..1784
                     /gene="radC"
                     /locus_tag="PKW60_10725"
     CDS             1089..1784
                     /gene="radC"
                     /locus_tag="PKW60_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPJ99950.1"
                     /translation="MTECTFSTALREMPEEERPRERLARLGAIALRDAELLAVLFRTG
                     TREEGAVALAERLLRQFGNLRALSRASLEELMQVKGVGTVKAIEIQAAVELGIRLARE
                     KDEERPKISSAEDVAELLSARYKQCEVEVFKALLLNTKNEVLKIVDVATGGLDATLAM
                     PRDVFRQAVRDAASAVIVCHNHPSGDPEPSREDIRITERLRDAAEIIGLRFLDHIVFG
                     HERYVSLQERGLL"
     gene            1781..2431
                     /locus_tag="PKW60_10730"
     CDS             1781..2431
                     /locus_tag="PKW60_10730"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPJ99951.1"
                     /translation="MIHPFSAWKTGAPYYVTCLAGGLVLAIFARNSPWVWAAAPLFLL
                     GGFALFFFRDPPRVSDAEPDEIVAPADGRIVGIDDLETSPYYDGPCKRIAIFLSLFNV
                     HINRSPVSGTVYRIEYKPGTFKAAMKAEASECNEANTLFMDSDQGRVTVRQISGLIAR
                     RIVCVAEEGQNLEQGQKFGMIRFGSRTELFLPPDAEICVALDEKVKAATSVVARFQ"
     gene            2428..3306
                     /gene="pssA"
                     /locus_tag="PKW60_10735"
     CDS             2428..3306
                     /gene="pssA"
                     /locus_tag="PKW60_10735"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPJ99952.1"
                     /translation="MKKLALRQQRRKRRTTRRKPINVVASALTTAGLYCGVVSIFKAI
                     DQDFKWAAYFIILAQVFDIMDGTFAKLTKTVSDFGKQLDSLADLASFGVAPAVLIYTA
                     YLHEAAALGLVGGKTGATMAIIFVICAALRLARFNVYQSEIRDYFVGLPTPAAAVCVA
                     TFVLFTVYFELQVAHWILTPLTLGLAYLMVSNFRYPKDILKDLVLAPRNAFRLLVLCG
                     IAIAVFHKAMDEHSPAIALFPLAAAYVLYGVGDRLYFFATRRYRQAEKAAIQPSGGVP
                     PSPGEGEPPAKSGDLL"
     gene            complement(3234..>4121)
                     /locus_tag="PKW60_10740"
     CDS             complement(3234..>4121)
                     /locus_tag="PKW60_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPJ99953.1"
                     /translation="AEPGVLPWYERFLERLPLDDLIRGLGWDEPGQSARGIVAQKLGT
                     FVRGHALQLLQSLWSRLVDATLTFAHLAKASGQALIRAVFFLANVALFAFVAGYLLKD
                     FDRLLAAARELVPPRHRPKTLEIAHKIDHQLRGFARGQMTVCLLLGLMYLIGLTVCGV
                     PFALPIALFGMFASLLPYLGALMTLLPALLMAYLGHGLDWHIAGVLVTFAVVQGLEGN
                     VLTPRIVGDKVGLHPVWVILAVLVFGSALGLLGVLLAVPLAASLKVLVVEGVQYYKRS
                     PLFAGGSPSPGDGGTPPEG"
BASE COUNT          823 a         1399 c         1162 g          737 t
ORIGIN      
        1 ccgtctcctc gcccagaggc aggaggaaga ggccgcggaa cgttccgcca accgcaagag
       61 ccaggtcggg tcaggcgacc gcagcgaacg catccgcacg tacaacttcc cccagaatcg
      121 actcaccgac caccgcatca agctcaccgt ccaccgcctc gaaaccatca tggaaggcga
      181 cctccaggag gtcatcgacg ccctgatcgc cgcggaccgg gccgcccgaa tccgcgagga
      241 atagtggcga ccatcgccga gagactcgca ggtgccgccg gcctgctggc ggccatcagc
      301 gacacgccca gactcgatgc cgaactcctg ctggcccatg cgctgggcat ttcccgcgcc
      361 gcgctcctgg cccggttcca ggacgaactc aacccacttc ccctgttcga cacattgctc
      421 ggccggcggc tgaactacga accactggcc tacatcctcg gaacctggga attcttctcg
      481 ctcgatttta tcgtagaggc gccgctcctc gtgccgcgtc ctgagaccga gcacgtggtt
      541 gaggctgttc ttgaacatat cggaacgcgg cccgctgcga tcctggaaat cggcaccggc
      601 accggctgca ttgccgtcag tatcgcgcac gcagctcgga atgcctcgat cattgccacc
      661 gatatcaaca gccacgccat cgcggtcgcc cggaagaatg ccgaacgcca tcgcgtcatc
      721 gatcgcctgt ccctgcgccc gggcaacctt tttgagccac tccagcccgg cgagggctcc
      781 tttgacgtga tctgctccaa cccgccttac attgaagaca cggcctggcc cggcctggac
      841 ccggttatcc ggctgcatga agatcctggc gccctgctcg ccggcctcga cggtctcgat
      901 gtcatccgcg ccctcgttgc cggagccgcc cggtggcttg cacccggcgc gctgcttgcg
      961 ttcgaaatcg gaatggggca atatgaacgg gtgagggacc tgctcgcccg ccatcaatac
     1021 gaacgcatcc ggttcgtgcg cgatctcgcg ggcattgaaa gagtggccgt agcacagaag
     1081 cctcgatcat gaccgaatgc accttttcga cagcgctgcg ggagatgccg gaagaagagc
     1141 ggccgcggga acgcctggcc cggctcggcg ctattgccct gcgcgacgcc gagcttctgg
     1201 cggtcctgtt ccgaaccggc acccgcgaag aaggggccgt ggcgcttgcg gaacgcctcc
     1261 tgcggcagtt cggtaacctt cgggcgctct cccgcgcttc gctcgaggaa ctgatgcagg
     1321 tcaagggcgt ggggacggtc aaagccatcg agatccaggc tgccgtcgag ctgggcatcc
     1381 gcctggcgcg tgaaaaggac gaggaacgcc caaaaatcag ttccgctgag gatgtagccg
     1441 agctgctcag cgcccgctac aaacagtgtg aggtcgaggt cttcaaggcc ctgcttctca
     1501 ataccaaaaa cgaggtgctg aagatcgtcg atgtggcgac cggcggactc gacgcaaccc
     1561 tcgccatgcc ccgggatgtt ttccgccagg cagtgcgcga tgccgccagc gccgtcatcg
     1621 tctgccacaa tcatcccagc ggcgatcccg agccgagccg tgaagacatc cgtatcacgg
     1681 aacggctccg cgatgccgcc gagatcatcg ggttgcgctt tctcgaccac atcgtcttcg
     1741 gccacgaacg ttacgtgagc ttacaggaaa gaggtctgtt gtgatacatc ccttcagcgc
     1801 atggaaaacg ggggcgccct attatgtgac ctgcctggcc ggcggcctgg ttctcgccat
     1861 tttcgcccgc aacagcccat gggtttgggc agctgcacct ttgtttctcc tcggaggctt
     1921 cgccctcttc tttttccgtg acccgccacg ggtatccgat gcagagcccg acgaaatcgt
     1981 cgctccagcg gacggccgca ttgtcggcat cgacgacctg gaaaccagcc cctattacga
     2041 cggaccgtgc aagcggatcg ccatcttcct ctcactcttc aacgtgcata tcaaccgttc
     2101 gcccgtcagc ggtaccgtct accgcattga atacaagccc ggcacgttca aagcagccat
     2161 gaaagcggaa gcgagcgaat gtaacgaggc caataccctc tttatggatt ccgaccaggg
     2221 gcgtgtcacc gtccggcaga tctccggact catcgcacgc cggatcgtct gtgtcgcgga
     2281 agaaggccaa aatctcgaac agggccagaa gttcggtatg atacggtttg gttcccggac
     2341 ggagctcttt cttccgccgg atgccgagat ctgtgttgcc ctggacgaaa aagtgaaggc
     2401 cgccacctcc gtcgtggcgc ggttccaatg aaaaagctag ctctcagaca acaacggcgg
     2461 aaacggcgta ccacgcggcg caaacccatc aatgtcgtcg ccagcgccct gactaccgcc
     2521 gggctctact gcggggtcgt cagcattttc aaagccattg accaggactt caaatgggcc
     2581 gcgtatttca tcatcctggc ccaggtcttc gacatcatgg acggcacgtt tgccaagctg
     2641 accaaaaccg tgtccgattt cggaaaacag ctggacagcc tggccgacct cgcgtcattc
     2701 ggcgtggccc cggcggtgct catctacacg gcctaccttc atgaagccgc cgcactcgga
     2761 ctcgtgggag gaaaaaccgg cgccaccatg gccatcatct tcgtcatctg cgccgcattg
     2821 cggctggccc gattcaatgt ctaccagagt gaaatacgcg attattttgt gggcttgccg
     2881 acacccgccg ctgccgtgtg cgtcgctacg ttcgtcctct tcacggtcta tttcgagcta
     2941 caggtcgccc actggatcct gaccccgctc accctcgggc tcgcctacct catggtgagc
     3001 aacttccgct acccgaaaga catcctcaag gacctcgtcc tcgcgcccag gaatgcgttc
     3061 cgtctgctgg ttctctgcgg tatcgccatc gccgtcttcc acaaagcgat ggacgagcat
     3121 tcgcccgcca tcgcattgtt tcctctcgcg gccgcctatg tgttgtacgg tgtaggcgac
     3181 cgcctctatt tcttcgccac ccggcgctac cgtcaggcgg aaaaggccgc cattcaaccc
     3241 tccggaggcg tgccgccgtc gcccggagaa ggcgagccgc ccgcaaaaag cggcgacctt
     3301 ttgtagtact ggactccctc cacgaccagc accttcagtg aggccgcaag aggcacggcg
     3361 agcaggactc ccagtaagcc cagcgcactg ccaaacacca gaaccgccag gataacccat
     3421 accggatgca gtccgacttt gtctcccaca atacgcggcg tcaaaacgtt cccttcgagg
     3481 ccctggacca ccgcaaaggt aacgagcacc cctgcgatat gccagtccag gccgtgcccc
     3541 agatacgcca tgagcagcgc gggcaacagc gtcatcagcg ccccgaggta gggcaggagg
     3601 ctggcgaaca tcccaaacag cgcgatcggc agggcaaagg gcacgccgca gaccgtcaac
     3661 ccgatcagat acatcagccc cagaagaagg cataccgtca tttggccccg ggcgaaaccg
     3721 cgcaattgat ggtcgatctt atgggcgatt tccagcgttt tgggtcgatg ccgcggcggc
     3781 accagctccc gtgccgctgc cagcagcctg tcaaaatctt tgagcagata gcccgcgaca
     3841 aacgcaaaca aggccacgtt ggccagaaag aacacggccc tgatcagggc ctggcctgac
     3901 gctttcgcca agtgcgcgaa ggtcagcgtc gcatcaacaa gccgcgacca caagctctga
     3961 agcaactgca gcgcgtggcc gcgtacaaac gtcccaagtt tctgggcgac aatgccgcgc
     4021 gcgctctggc ccggctcatc ccagcccaga ccacggatca ggtcgtccag cggcagccgt
     4081 tccaggaatc gttcatacca cgggagaacc cccggttccg c
//
LOCUS       DAOPBQ010000338         4103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17823_length_4103_cov_4.536561, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000338 DAOPBQ010000000
VERSION     DAOPBQ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17823_length_4103_cov_4.536561"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PKW60_10745"
     CDS             complement(<1..521)
                     /locus_tag="PKW60_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPJ99954.1"
                     /translation="MQSEIVMIGTELLLGQIADTNATFLGQVLAENGINLYQKTTVGD
                     NPERIKRALESALARADLVLTSGGLGPTEDDITRECIAEVFGRRLELRPELVEQLEAR
                     FQRIGRPPTGNNLKQAYAPEGAVALENPNGTAPGLLIEDARGIIIAMPGVPRELKPML
                     KDQVLPFLRKRFN"
     gene            1025..2860
                     /gene="dnaG"
                     /locus_tag="PKW60_10750"
     CDS             1025..2860
                     /gene="dnaG"
                     /locus_tag="PKW60_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPJ99955.1"
                     /translation="MRQKWRGGLARSYSNQYFALHTLIQLLATYNKEIINEVLRATEI
                     VDVVRRALELKPASGGRFVALCPFHHEKTPSFTVNRERQTYHCFGCGKGGDAISFLCE
                     FDGLSFIEALRRLADDAGIRLPAMSASESGQEKQRARLLEVNAFAAKFYAGNLHDPAK
                     GEKGQQYLDTRKLTPALVERFGLGYAGGAWSTFTDAARLHGFDERLLEASGLVRRSER
                     GTCYDFFRDRLMVPIRDTSGRVLAFGGRDLSGESAAKYINTPENGLYKKSHVLYGVYE
                     AREAMRQSKRAILVEGYFDLLRPFDEGLENVVATCGTALTIDQARLLRRYVSEVVLVY
                     DGDAAGIQAAIRGVSILTAAGLTVRALSLPDGQDPDDFIQKSGISAFSQRVDSAPDFV
                     TFYVEMSHSRLDTIEGRTSVARELFAILTHLDDELRREEYLKRMAQALHIDEWTTRNE
                     YHKTVRQSQRRDRRSAIHETKIPKINADDCDFLAALLNEAGLREKVQRALDNVTLPQT
                     PFREVLTRVMQASGPGTVHEFEDDTARQLYTAATSQEPPDSARAEVLVEKRLTRLQRD
                     ALELKSAKLQEAIRAAEREKNSAQVMELLQEKLRIDKRIQELGAA"
     gene            2940..>4103
                     /locus_tag="PKW60_10755"
     CDS             2940..>4103
                     /locus_tag="PKW60_10755"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HPJ99956.1"
                     /translation="MPISSKKQTKLDGNGKQKALDLAKGKLNKLTYDDLNLVLPEGAT
                     AEDIDEMMAMLSRMDIDIVDEFRVDSESQKKQQQRDKQLRRAQARREAAQTRLERADD
                     PVRMYLREMGRVPLLTKDQEVAIAKRIEEAELDLIDVLHHTPYTMKEVGMIAARILAG
                     KLDFAQITDIELPKQQQELINQLPKLMEQITTADAEIEQQDKRLRRSGLSEKSIQNID
                     ARVASARERQINAIKALKLKTKEMMKIARKIKSLKRRIASARDEIDRIEREVGFKAPQ
                     IHEMAVRLRRTPSSAKKLGVDRDLIFDAERRLLLAQRKIDQIESDAKLGADAITVLID
                     TIRDKEEKVYKAKMELVEANLRLVVSIGKKYTNRGMSFLDLIQEGNIGLMKAVD"
BASE COUNT          953 a         1261 c         1133 g          756 t
ORIGIN      
        1 aaattaaagc gcttacgaag aaatggcagc acctggtcct tcaacatggg cttcaattcc
       61 cgcggaacgc ccggcatcgc gatgatgatg ccgcgtgcat cctctatcag taagcccggt
      121 gcagtgccgt tcggattttc caacgctacc gccccctcgg gcgcataggc ctgtttcaag
      181 ttgtttcctg tgggcggcct gccgatgcgc tggaaccggg cctccagctg ttccaccagt
      241 tcggggcgca gttccaggcg ccgcccaaac acctccgcga tgcattcccg ggtgatgtcg
      301 tcctctgttg gccccagccc cccggaggtc agcaccaaat ccgcccgggc aagcgccgat
      361 tccagcgccc gtttgattcg ctccggattg tcacccaccg ttgtcttctg ataaagatta
      421 attccgtttt cggcaagaac ctgccccaaa aaagtcgcat tggtgtcggc tatctgcccc
      481 aggagcagtt cagtgcctat catgacaatt tcagattgca tcgggtccct ttccatgttg
      541 gacgcgcctg gcagacgcgg aatgccacca gaataggccc gcgcgcgcgc cacttcaatc
      601 tccattttcc ccgccgctca cgccaaggcg ggccgcacgg gggtgggcgg ccactggggg
      661 ctggccatgc ccaaccaaac cacacccctc atgactgccc aatgcgccca gatggaattt
      721 tcgccggcca gggcccacaa acccgggatt catttgggga gaatcttgcc aaagacctca
      781 tttgacagtc tgaaaaaaat gtggtacatt tttagcgatt tgacagagca gcgccagagt
      841 ctgccttgtc gaacccagtc gtacttttct caattaaggt ctgtgtgtaa agacagttgg
      901 cacagggcaa atggcttctc ttcctctgca agcctgtaac acaagtaata acatggagtt
      961 ccgttgattt tggccatctt ctcgcggatt gcggccgaga aattcgggtg tgcggggccg
     1021 ggttttgcga cagaaatggc gtggcggatt ggcccgatcg tattcgaatc agtatttcgc
     1081 gctgcacacc ctcattcaac tcttggccac atacaataaa gaaattatta acgaagtgct
     1141 gcgagccact gaaattgtgg atgtggttcg ccgtgcgctc gaactcaaac cggccagcgg
     1201 tggccggttt gtcgcgttgt gcccatttca ccatgaaaag accccttctt tcacggtgaa
     1261 ccgcgagcgc cagacctacc attgcttcgg ctgcggaaaa ggcggagacg ccatctcgtt
     1321 tctctgcgaa tttgacgggc tgagcttcat cgaggcactg cgccgtcttg ccgacgatgc
     1381 tggaattcgc ctgccggcga tgtccgcgag cgaaagcggc caggagaagc aacgcgcccg
     1441 cctcctggaa gtcaatgctt ttgcggcgaa gttttatgcc ggtaacctcc acgacccggc
     1501 caaaggtgaa aaagggcagc agtatctcga tacccgcaag ctcacccccg cacttgttga
     1561 acgtttcggc ctgggatatg ccggtggggc ttggagtaca ttcacggatg cagcccggct
     1621 ccatggcttc gatgagcgtc tcctggaagc ctccggcctc gtccggcgca gtgagcgtgg
     1681 aacgtgttac gatttttttc gcgacaggct catggtgcct atccgcgata cgtccggccg
     1741 cgtgcttgcc tttggcgggc gcgacctcag cggcgagtca gcggccaagt acatcaatac
     1801 acctgaaaac ggcctgtata agaaaagcca cgtgctgtac ggcgtgtacg aagcccgtga
     1861 ggccatgcgg cagagcaaac gcgccatcct ggtcgaaggc tattttgacc tccttcgtcc
     1921 ctttgacgag ggcctcgaaa acgttgtggc cacctgcggg acggccctga ccatcgacca
     1981 ggcccggctg ctccggcgct atgtgtcgga ggtcgtgctc gtatacgacg gcgacgctgc
     2041 cggaattcag gcggcgatcc ggggtgttag catcctcacg gcagccgggc tgaccgtccg
     2101 ggccctttcg ctgccggacg ggcaggaccc ggatgatttc attcaaaaaa gcggtatcag
     2161 cgcattttcg cagcgggtcg attccgcgcc tgattttgtg accttttacg ttgagatgag
     2221 ccacagccgc ctggatacta tcgagggccg gacttcggtc gcccgagagt tgtttgccat
     2281 tttgacccat ctcgacgatg agctccgccg cgaagaatac cttaagcgca tggcgcaagc
     2341 cttgcatatt gatgagtgga caacgcgaaa cgaataccac aaaaccgtcc gccagtcgca
     2401 gcggcgggat cggcgttccg ccatccacga gacgaaaata cccaagatca acgccgacga
     2461 ttgcgacttc ctggcggccc tgctcaacga ggcgggattg cgcgagaaag tccaacgggc
     2521 tctggacaac gtgacattgc cccaaacgcc ttttcgtgag gtgctgacgc gggtgatgca
     2581 ggcatccggc cccggcacgg tgcacgaatt cgaggacgat acggcacggc agctgtatac
     2641 cgccgcgacc agccaggaac cgcccgacag cgcccgcgcg gaagtcctcg tcgagaagcg
     2701 cctcacccgg ctccagcgcg atgccctcga gcttaaatcg gcgaaattac aggaagccat
     2761 ccgcgcggcc gaacgcgaaa agaacagcgc gcaggttatg gaattgcttc aagaaaaact
     2821 acgcatagat aaaaggatac aggaactcgg cgccgcgtag attcccgcag cgcttgaaaa
     2881 tttgcagtgg ccccagacaa ccgaggaaac agctgacgcg ttttggagtt gagcacacaa
     2941 tgcctatcag cagcaaaaaa cagaccaaac tcgacggaaa cggaaaacag aaggccctgg
     3001 atctggcgaa gggaaaactg aacaaactca cctatgacga cctgaacctg gtcttgcctg
     3061 aaggcgccac cgccgaagac atcgatgaga tgatggccat gctcagccgc atggacatcg
     3121 acatcgtaga cgagtttcgc gtcgactccg agtcgcagaa aaaacagcag cagcgcgaca
     3181 aacagctgcg gcgcgcccag gcccggcgcg aagcggccca gacccggctc gagcgcgccg
     3241 atgaccccgt ccgcatgtac ctgcgcgaga tgggccgcgt acccctcctg actaaagacc
     3301 aggaggtcgc catcgccaaa cgcatcgagg aagccgagct cgacctcatc gatgttctgc
     3361 accatacccc atataccatg aaagaagtag ggatgatcgc ggcccgcata ctcgccggta
     3421 aactggattt cgcccaaatc accgacatcg aactccccaa acagcagcag gagctcatca
     3481 accagctccc aaaactcatg gaacagatca ccacggccga cgccgaaatc gagcagcagg
     3541 ataaacgcct gcgtcggtcc ggactgtccg aaaaaagcat ccaaaacatc gacgcgagag
     3601 tggccagtgc gcgcgaacgt caaatcaatg cgatcaaggc gctcaaactc aagaccaaag
     3661 agatgatgaa aatcgcccgc aagatcaaga gtctcaagcg gcgcattgcc tccgcgcgcg
     3721 acgagatcga ccgcatcgaa cgggaagtgg gattcaaagc gccgcagatc cacgagatgg
     3781 ccgtccggct ccgccggact ccgtccagcg ccaaaaaact cggcgtcgac cgcgacctca
     3841 tcttcgatgc cgaacgccgc ctcctgctcg cccagcgcaa gatcgaccag atcgaatccg
     3901 acgccaaact cggcgccgac gcgatcacgg tgctgatcga caccatccgc gacaaagaag
     3961 aaaaggtcta caaagccaaa atggaactgg tcgaggccaa cctgcgcctc gtcgtcagca
     4021 tcggcaaaaa atacaccaac cgcggcatgt ctttcctcga cctcatccag gaaggcaaca
     4081 tcggcctcat gaaagcggtc gat
//
LOCUS       DAOPBQ010000339         4103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17824_length_4103_cov_3.724555, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000339 DAOPBQ010000000
VERSION     DAOPBQ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17824_length_4103_cov_3.724555"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="PKW60_10760"
     CDS             complement(<1..1436)
                     /locus_tag="PKW60_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HPJ99957.1"
                     /translation="MWHAAIWRRGFALAVLMAGILAGSAPAQHYLSPLSLAVGPEQSN
                     LYVAAYTGSQIVVLSLAESAVTSTFSLPGAPTGLCVSADGAELYVTVETPEGALLALN
                     RADGTVRASLPAGHGPTAPVASPDGKLVYVCSRFDNLVTVMDIAAGQELARIPVNREP
                     IAAALTPDGKWLCVANHLPVGPSDADYVSATISIIDTTERSVAATVILPNGSTGLRGL
                     ALSPDGAYAYATHLLGRYQLPTTQLERGWMNTNAVSIIDVAGQKLLNTVLLDDVDLGA
                     ANPWGVLVSADGAYLCVAHAGTQEISVIDRAALHAKLASVAAGEPVSEVSRTSEDVPN
                     DLAFLVGMRRRIALAGNGPRGNAMAGTRVYAAEYFSDSVGVVDVSPDARPRATSIALG
                     PAQEMSIVRKGEMLFNDADLCFQKWQSCASCHPDMRADALNWDLLNDGMGNPKNTKSL
                     FLSHQTPPVMVSGVRDRAVLLERMRQDIP"
     gene            complement(1700..>4103)
                     /locus_tag="PKW60_10765"
     CDS             complement(1700..>4103)
                     /locus_tag="PKW60_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPJ99958.1"
                     /translation="YSDTPHYFSRLLFHMNPDGTNQMEFYGSNSFWPNSIFYARPIPN
                     HPTKIVAIVSGHHGVARMGELTVFDPAEGRREADGAVQRIPGYGKPVDPIIADELVQN
                     SWPKFLHPYPLSDKYFLVSCKPSPRAPWGIYLADIFDNLVLLKEEPGFALLEPIPFRP
                     TTAPPEIPDKVHLDSKEATVYLADIYEGDGLRDIPRGTVKKLRLFAMHYAYNRMGGHV
                     HIGVEGPWDVHRILGTVPVFEDGSAVFTVPANVPIAVQPLDEQGRALQVMRSWFTAMP
                     GEILSCVGCHEQQNMGPPSALSLASRALPAAIEPWHGPARGFSFKRDVQPVLDQFCVG
                     CHQGQEGRPDFARKDENGWNNFTPSYLALHPYVRRPGPESDYHLATPMEWHAGTSELI
                     QMLEKGHFGVKLDEEAWDRLFTWIDLNVPDHGTWHEQQPIPHDFHARREYMLTTYANR
                     TEDFEVIPEGLGAPREFVRPERPATRQDATVRVEDWPFSDEEARALQRAAAGAVAAVD
                     AAGELHRKTVDLGGGMTMDFVLIPPGDYVMGSVDGALDEQPPCRVAIDQSFWMGVTEV
                     SNAQYACFDPAHDTGTIDQHNKDHTRPGYAANLPEQPAARVSWDDAMAFCEWLTGRAG
                     APCTLPTEAQWEWACRAGSGDAFFYGGMDADFSSFANLADQSIKLLAVAGIDPQPIPN
                     PSPYEDFLPKDTRFNDGERIVAAVGKYQPNPWGLMDMHGNVAEWTRSSFAAYPYLEDG
                     RNDPDSRQNKVVRGGSWFDRPHRATSSFRLAYKPYQKVYNVGFRVIIPVDTPKQVNIA
                     RN"
BASE COUNT          765 a         1276 c         1283 g          779 t
ORIGIN      
        1 gggatgtcct ggcgcatgcg ttccaggagc acggcccggt cgcgcacgcc ggacaccatc
       61 accggtggcg tctggtgcga caggaaaagg ctcttggtgt tcttggggtt gcccatgccg
      121 tcattcagaa gatcccagtt cagggcatcg gcgcgcatgt cgggatggca gctcgcgcaa
      181 ctctgccact tctggaaaca gaggtcggcg tcattgaaca gcatttcacc tttgcgcacg
      241 atcgacattt cctgcgccgg ccccagcgcg atggaagtcg cgcgcggccg tgcatccggg
      301 ctcacgtcga ccacgccgac gctgtcgctg aagtattccg cggcatagac gcgggtaccc
      361 gccattgcgt tgcctcgggg tccatttccg gccagtgcaa tgcggcggcg catgcccacc
      421 agaaatgcca ggtcattggg cacgtcttca gaggtacgcg acacttcgga tacgggttca
      481 ccggccgcca cgctggccag cttggcatgc agcgcggcgc ggtcgatcac actgatttcc
      541 tgggtgcccg cgtgggcaac gcagagatat gccccgtcgg cgcttaccag caccccccag
      601 gggttcgcgg cgcccaggtc gacatcatcc aaaagaaccg tgttcaacag cttctggccc
      661 gccacgtcga tgatgctcac cgcattggta ttcatccagc cgcgttcaag ctgcgtggtg
      721 ggaagctgat agcgtccgag aaggtgtgtc gcataggcat aagcgccgtc cggcgacagg
      781 gcgagcccgc gcaggccggt ggagccgttc ggaaggatga cggtggccgc cacgctgcgc
      841 tccgttgtat cgataatgga aatagtggcc gagacatagt ccgcgtcaga cggccccacg
      901 ggcagatggt tggccacgca gagccatttc ccgtcgggcg tcagggccgc cgcaatcggc
      961 tcgcgattca cgggaatccg ggccagctcc tgaccggcgg cgatatccat cacggtcacc
     1021 aggttgtcga aacggctgca cacgtacacg agttttccgt cggggctcgc cacgggcgcc
     1081 gtcgggccgt gcccggcggg gaggcttgcc cgaaccgtcc catcggctcg attcagggcc
     1141 agcaacgccc cttcgggcgt ctcgaccgtc acatacagct cagctccatc ggcggagaca
     1201 cacaggccgg tcggtgcgcc gggcagggaa aatgttgagg tgactgcgga ctctgccagg
     1261 ctcaatacaa cgatctggct gcctgtgtac gcagcgacgt acaggttgct ttgttccgga
     1321 cccaccgcga gcgacagcgg cgacaaatag tgttgcgccg gggcactgcc agctaaaatt
     1381 cccgccatca gcactgcgag cgcaaacccg cggcgccaga ttgccgcatg ccacatactc
     1441 cagtccccct tagtcgtgcg ttgccgtgtc ttgcatcctg atttccgttc acagacgggg
     1501 cagtctacac cgtcgcggcg ccaagtgtat tcgattgtac ccattcttcc ttcaagattc
     1561 taaccgcggc ctttgcggga tttgcggtgc cgcaaaggcc gcgtaaaggc tccggcctgc
     1621 gcactgcgct ctaaatgccc gcttgagagc ggcactcctc aacacatccg aaacaggctg
     1681 cgtacccgtg aattgccgct taatttcggg cgatattgac ctgttttggc gtatcaacgg
     1741 gtatgatcac gcggaatccc acgttataca ccttctgata tggcttatac gccagccgga
     1801 aggacgacgt ggcccggtga ggccggtcaa accaggagcc gccccgcacg actttgtttt
     1861 ggcgcgaatc cgggtcattg cggccgtctt ccagataggg atacgcggcg aacgagctgc
     1921 gggtccattc cgccacattg ccgtgcatat ccatcaatcc ccagggattg ggttgatact
     1981 tgcccacagc ggccacgatg cgttcaccat cgttgaagcg ggtgtctttc gggagaaaat
     2041 cctcgtaggg gctgggattg ggaataggtt gaggatcgat gcccgcaaca gccagaagct
     2101 tgatcgattg gtcggcgaga ttggcgaagg atgaaaaatc cgcatccatc ccgccgtaga
     2161 aaaacgcgtc gccagaaccg gcccggcagg cccattccca ttgcgcctcg gtgggcaggg
     2221 tgcagggagc gcccgcgcgc ccggtcagcc actcgcaaaa tgccatggcg tcatcccacg
     2281 aaacccgcgc cgccggctgc tcgggcagat tggctgcgta tcccgggcgg gtgtggtctt
     2341 tgttgtgctg atcgatggtc ccggtatcgt gcgcggggtc aaaacacgca tactgcgcat
     2401 ttgacacttc ggtgacgccc atccagaacg actggtcaat ggccacccgg cagggaggct
     2461 gctcgtccag cgctccgtct acgctgccca ttacgtagtc ccccggggga atgagcacga
     2521 agtccatcgt cataccgccg cccaggtcaa cggtcttcct gtggagctcg cctgccgcgt
     2581 ccaccgcggc cacggcccct gccgccgcgc gctgaagcgc gcgcgcctcc tcgtctgaga
     2641 acggccagtc ttcaacgcgc accgtggcgt cctgccgtgt agcgggccgc tcaggacgca
     2701 cgaattcacg cggggcgccg aggccttcgg ggatgacttc aaaatcttcc gtacggttgg
     2761 cgtaagtggt cagcatgtac tcacggcgcg catggaaatc atgagggatg ggctgttgtt
     2821 cgtgccaggt accgtgatcg ggcacgttga gatcgatcca ggtgaacagc cgatcccagg
     2881 cctcttcatc caatttcacg ccgaaatggc ccttctcgag catttgaatc agttcgctcg
     2941 tgcccgcgtg ccactccatg ggcgtcgcca gatggtaatc actttcggga ccaggccggc
     3001 gcacatacgg gtgcagggcc aggtaggacg gcgtgaagtt gttccagccg ttctcgtctt
     3061 tgcgggcaaa atcagggcgc ccctcctgcc cctggtggca acccacgcaa aactggtcaa
     3121 gcacaggctg gacatcgcgt ttgaagctga acccgcgcgc cggcccgtgc cacggttcaa
     3181 tcgccgcggg gagcgctcgc gaagccaggc ttagagctga aggcggtccc atattctgtt
     3241 gctcgtggca gccgacgcac gacaggattt ctcccggcat cgccgtgaac cagctccgca
     3301 tgacctgcag tgcgcggccc tgctcgtcaa gtggctgcac ggcgatcggg acatttgcgg
     3361 gcacggtaaa taccgccgaa ccgtcctcaa acacgggcac cgtaccgaga atgcggtgca
     3421 cgtcccaggg gccttccacg ccgatgtgga cgtgcccgcc catccggttg tacgcgtaat
     3481 gcatggcaaa cagccgaagc ttcttgacgg ttccgcgggg gatgtctctc aggccgtccc
     3541 cttcgtagat gtccgcgagg tagacggtcg cttccttgga atcgaggtgc accttgtccg
     3601 gaatttcggg cggcgccgtt gtgggccgga acggtatcgg ctccaggagg gcgaatccgg
     3661 gctcttcttt gagcaacacc aggttgtcga agatatcggc gagatagatc ccccaggggg
     3721 cccggggcga aggtttgcac gacacgagga aatacttgtc gctcagcgga tacggatgca
     3781 ggaatttcgg ccaggaattc tggacgagct cgtcggcaat gatcgggtca acgggtttgc
     3841 cgtagccggg aatgcgctgc actgcgccat ccgcctcgcg ccgcccttcg gcggggtcaa
     3901 acacggtgag ttcgcccata cgggccacgc cgtgatggcc ggagacaata gcgacgatct
     3961 tggtgggatg attgggtatc ggccgcgcat agaagatcga attcggccag aacgagttgc
     4021 tgccgtaaaa ctccatctgg ttggtgccgt ccgggttcat gtgaaacagc agacgcgaga
     4081 aatagtgcgg agtgtccgaa tac
//
LOCUS       DAOPBQ010000340         4101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17837_length_4101_cov_3.492832, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000340 DAOPBQ010000000
VERSION     DAOPBQ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17837_length_4101_cov_3.492832"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PKW60_10770"
     CDS             <1..474
                     /locus_tag="PKW60_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99959.1"
                     /translation="DFEHESLYCPPDKETGRPAVARCGNVIHFSFPLFLGYMRDAVVP
                     HRVLLENALRQLLPQPMLEVANFPSFGRATLTSREQTRLVHLHTYVPESRGSIEMVEE
                     PIVVSDVAVGLRSDGRNVSSVTLAPGGETLPFTRDGDYVRFTVPRVEGYQLIVVE"
     gene            936..1580
                     /locus_tag="PKW60_10775"
     CDS             936..1580
                     /locus_tag="PKW60_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99960.1"
                     /translation="MNKVMWVPLALFLSVALAGTAWAAGAAPKDPNQDWEKYPEPALQ
                     VYKTTGRGIHALVYQSLRSLKEGNERLPILGSVLVFEGVGTGLVELGTSMYTGMAGSK
                     PHHPYNQLSRPNRVLNADPVLRNTRQVGGTAALTAGAGLPVVLGLYAGQEVVDEFPRE
                     ADKVEELENIRRPVEKKRMTMPDAQRRYVPQRVSYGRQIERGRGNLLKQLRYER"
     gene            1667..3487
                     /locus_tag="PKW60_10780"
     CDS             1667..3487
                     /locus_tag="PKW60_10780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPJ99961.1"
                     /translation="MVSLIHKHFWLVLLFAALFAAFFELGRMDVYTDNEGQRATPPAE
                     MLRTGDYLVPTINGTTYLAKPPLLYWAIAAVYRMTGRISALTARIPTAACAVGMVLAV
                     YLVFRKRLGELAARFAALALLASPYFLERSRWAELDVPLTLATFLALAACWRAFEEDS
                     LAGQTRFTLLGGLAMGAAIMLKGPVPALFLGAAWAAYLLTHGETRGREFAGLAVVIVA
                     ALTLEYLLKFTGILAFPLALMSTIVCGAIWAWKAAPQLRLKVSIISGTVFLVGALLAL
                     PWALAVLYDQGWDNIRSLLHSEVITRTHSATEINSGSPVYYLVALPLLLAPWSFLIPL
                     HFSRTERHEGGINYRFCTAAAWISILLFSLIAGKEYEYILPAFPFLMGATGVTLERFY
                     RGSLTGWVKRWAALWARAAVIILALFAIAMMIYAVSTDHPAIFVVEAVGLSALAAAAG
                     VWGLRRRTFRLHCVVFQALAVLVVGLLASRSFFYTGANSPREIAETTGRLLQSGYRVE
                     AGWFRHPWTPAFAFYAATPVPMQMNFDFVKQRLAETTPYFLLIRKKDLENGPIMLDPS
                     TYRVLMGPYTSKEYLLIGNTGLPPSVAPPEPANLSTNSLR"
     gene            3547..3920
                     /gene="rnpB"
                     /locus_tag="PKW60_10785"
     ncRNA           3547..3920
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKW60_10785"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          774 a         1252 c         1225 g          850 t
ORIGIN      
        1 gacttcgagc acgaaagcct ctattgcccg cccgacaagg aaaccggccg ccccgctgtt
       61 gcccgttgcg gcaatgtgat ccatttcagc ttccctctct tcctcggata catgcgtgat
      121 gccgtggtgc cccaccgcgt cctgcttgaa aacgccctgc gacagctgct gccgcagccg
      181 atgctcgaag tggccaattt cccctcgttc ggccgcgcca ctttgacctc ccgcgagcag
      241 acccggctgg tccatctgca cacgtatgtg ccggaatccc gtggaagcat cgaaatggtc
      301 gaagagccga tcgtggtgag cgatgtcgcc gtgggccttc gaagtgatgg caggaatgtg
      361 agttcagtga cgctggcgcc gggcggcgaa accctcccct tcacacgcga cggcgactat
      421 gtgcgattta ccgtgccccg tgtggaggga taccagctca tcgtggtcga ataatttgca
      481 tcggcgtgtt gtcgccatgc ccaacccgca cgttcgcctt ccggttggag gggcggcccc
      541 ctgtggccgc ccatccggat agcccatgcg gtggccctga agaagagcag gcacaggggc
      601 ctgcgctacg caaaataaga acgcaggcaa gatgcctgcg ctacgcaaaa taagaacgca
      661 ggcaagatgc ctgcgctacg cgttgcgaaa accaggacga tctatggcag ttcgcttcaa
      721 ttttccagca acattgcccc aaatgcgccc attccacctc ggcccatcca cacactcaaa
      781 taaaacaaca acttacgcaa atcaaacaaa tacttcggtg aaagccctgc cgtgcaagcc
      841 tcttggaatc ctgagatttt tcgggtacac taagttaaag tcttacagac agatgcacgg
      901 ttcgtatggg gacactgaac tgaggtggaa gtcttatgaa caaagtgatg tgggtaccgt
      961 tggcactgtt cttgagcgtg gcgctggcag gcacagcgtg ggccgcgggt gctgccccga
     1021 aagatccgaa ccaggactgg gagaaatatc ccgagcccgc attgcaggtc tacaagacca
     1081 cggggcgagg catccatgcc ctcgtctatc agtccctccg ttccctgaag gagggcaacg
     1141 aacgcctgcc catcctcggc agcgtcctgg tgtttgaagg cgtcggaacc ggcctggtgg
     1201 agctgggcac gagcatgtac acgggtatgg caggttcgaa gccgcaccat ccatacaatc
     1261 agctgagccg gcccaaccgg gtcctcaatg cggacccggt cctccgcaac acccggcagg
     1321 taggcggcac ggccgctctg accgcgggtg cgggcctccc cgttgtgctg ggactctatg
     1381 ccggtcagga agttgtggac gaattccccc gcgaagcgga caaagtcgaa gaactcgaga
     1441 atatccgccg gcccgttgag aaaaaacgga tgaccatgcc cgacgctcaa cggcggtatg
     1501 tgcctcagcg tgtcagctac gggcgccaga ttgagcgagg ccggggaaat ctgctgaagc
     1561 agctgcgata cgaaagataa ccgtgggctg cagtcggcgg cgttcagtgt tctggagaaa
     1621 accgttccag cggtgagggc cgtctcgcga ggttcgttgg ctattcgtgg tgtcacttat
     1681 tcataagcat ttctggctgg ttctgctgtt cgcggctctc tttgcggcct ttttcgaact
     1741 gggccgcatg gacgtgtaca ccgacaacga agggcagcgg gccacgccgc cggccgagat
     1801 gctgcgaacc ggcgattacc tcgtccccac catcaacggc acgacttatc tcgccaagcc
     1861 tcccctcctg tattgggcaa ttgcggcggt ctaccggatg acagggagaa tctccgcgtt
     1921 gacggcgcgt atccccaccg ctgcctgcgc ggtcggaatg gttttggcgg tgtacctggt
     1981 ttttcgaaag cggctgggcg aactggctgc ccggttcgcc gccctggcgc tgctggcgtc
     2041 gccctatttt ctcgagcgct cccgctgggc ggagcttgat gtgcccttga ccctggctac
     2101 cttcctggcc cttgctgcct gctggcgtgc tttcgaggaa gacagtctcg ccgggcagac
     2161 gaggttcacg ctgctcggcg gcctggccat gggtgcggca atcatgctga aagggccggt
     2221 ccccgcactc ttcctgggcg ccgcatgggc ggcatacctg ctcacccacg gcgaaacccg
     2281 tggccgggag ttcgcaggac tggctgtcgt catcgtagcc gccctgaccc tcgaatacct
     2341 tcttaaattc accggaatcc tcgcctttcc gctcgcactg atgtccacga tcgtatgcgg
     2401 tgcgatttgg gcctggaagg ctgcgcctca gctccgtctg aaggtcagta taatctccgg
     2461 caccgtgttt ctcgtgggcg cgttgttggc ccttccctgg gctctggcgg ttctgtacga
     2521 ccagggctgg gacaatattc gcagcttatt gcactcggag gtcattacga ggacccattc
     2581 cgccaccgag attaacagcg gctccccggt ctactatctc gtggccctcc cactgctgct
     2641 cgctccgtgg tcgtttctta tccccttaca tttcagccgg acagagaggc atgaaggtgg
     2701 aatcaactac cgtttttgca cggctgccgc ctggataagc attttgctct tttcgctgat
     2761 cgcaggtaag gaatacgagt acattcttcc ggctttccca tttttgatgg gcgctactgg
     2821 cgtcacgctt gagcgatttt atcgcggcag ccttacgggt tgggtcaaac gatgggccgc
     2881 tctatgggca cgggctgcgg tcatcatctt ggcgcttttc gctatagcca tgatgattta
     2941 cgctgtgagc acggaccatc cggccatctt tgttgtggaa gccgtggggc tgagcgcctt
     3001 ggccgccgct gctggcgtat ggggactgcg gcggcgcacg tttcggcttc actgtgttgt
     3061 gttccaggcg ttggccgtgc tggtcgtggg gctcctcgcc tcccgctcgt ttttttacac
     3121 cggcgcgaac tccccccggg agattgccga aaccactggc aggctccttc agtccggcta
     3181 ccgtgtggaa gcgggatggt tccgccatcc ctggaccccc gcgtttgcat tctatgccgc
     3241 gacccccgtg ccgatgcaga tgaactttga tttcgtgaag caacggcttg ccgaaacaac
     3301 tccctacttt ttgctgattc gaaagaaaga cctggaaaac ggcccgatta tgcttgatcc
     3361 ctcaacgtat cgggtgctca tgggcccgta caccagcaaa gaataccttc tgatcgggaa
     3421 caccggcctc cctcccagcg tggcaccgcc ggagcccgcg aatttatcca ctaattcact
     3481 tagataggcg ttgggcttga atagtacttc atcttttctg ccaggtactg tataataaac
     3541 ctcgagtgag tgggccgggc ggccgcggtg ttgccggaag gcggcatcga ggaaagtccg
     3601 agctccacag ggcagggtgc ttcgtaacgc gaaggcgggg tgacccgacg gaaagtggca
     3661 cagaaaacac acagcctacg tctgccatgc gcggaccggc aatggtgaaa aggtgcggta
     3721 agagcgcacc agcggcttgg cgacaggccg gcttgctaaa ccccacctgg agcaattcca
     3781 aatagggggg ctatggagtg gcccgctccg cccccgggtt ggaagcttga ggcctgcggc
     3841 gacgcaggtc ctagaggaat ggccgcacag ggacggttcg ccgtcctgga cagaactcgg
     3901 cttacaggcc cactcactat ctggggcatc gggtcccgca cacacgacgc cgaggggcca
     3961 cggttcatat gcacgccgga aaaccaccgg gggaggctgg atatgaacaa gaatcccggg
     4021 tcagcacgat cccgagcccg gccttcgccg ccctccctgg ccacccgggg ggggcagtac
     4081 ctcacgttcg cgttgggccc g
//
LOCUS       DAOPBQ010000341         4095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17868_length_4095_cov_4.428713, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000341 DAOPBQ010000000
VERSION     DAOPBQ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17868_length_4095_cov_4.428713"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /gene="glgC"
                     /locus_tag="PKW60_10790"
     CDS             <1..342
                     /gene="glgC"
                     /locus_tag="PKW60_10790"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of ADP-glucose and
                     diphosphate from ATP and alpha-D-glucose 1-phosphate;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPJ99962.1"
                     /translation="DEGSRIEDATITDSIIGIRTIVGRGVVINRSIIMGEDFFEEECP
                     PGRIPIGIGEGTHIEGAIVDKNTRIGRNVRIYAADALPDQIGDGWAIRDGIVVILKGA
                     EIPDGTVIGKA"
     gene            448..1776
                     /locus_tag="PKW60_10795"
     CDS             448..1776
                     /locus_tag="PKW60_10795"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPJ99963.1"
                     /translation="MPDKKKDEASDFRYETGEARIPWAAVGEQIREDDILALIKFLLR
                     PARGEEKKYDAHLKKVAAQLRDLGAVSTPAGKLSLAGNVQALEEATARFLGAKHAVFI
                     TNATAGFEIAFKYAGLKPGDEVIAPALTFIATIAYPLAMGAKVVLSDVEPRSLNMDPA
                     DVARKITPRTKAIIPVHLGGYPVDMAPLMKLARKHNITVIEDAAHAFGASYRGKMIGT
                     IGHFGSFSFHEVKNVTSLGEGGILTSTTAFGRELRRARFLGLDLTHPIPNWLYDVPAF
                     DGKDGPFFTGNYSTTEIQALGLLTQMKRLQRIIAKRKKAAQYLDKRFAKVDGLLTPPG
                     DDARIKSTYHLYLLQIEPGKVGGDIQALKKKLDARGVVQIPHFAPLYKFSVMRQLGYD
                     TAAMEKTCPVAEEAFQHRFTHLPLYDFTREQLAYLADAVIESIGEMKRGV"
     gene            1931..3232
                     /gene="eno"
                     /locus_tag="PKW60_10800"
     CDS             1931..3232
                     /gene="eno"
                     /locus_tag="PKW60_10800"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPJ99964.1"
                     /translation="MTRITDVRAREILDSRGNPTVEVEVYLASGFRGRAAVPSGASTG
                     ENEALELRDKDPKRYLGKGVKKAVQNVNDIIAEELMGMEATDQRGIDSLLISLDGTKN
                     KGKLGANAMLGVSLAAARAAAEALEVPLYKYIGGVNAHVLPVPMMNILNGGQHADNNV
                     DVQEFMVMPVGAKSFSEALRMGAEVFHALKSVLKKQGLNTAVGDEGGFAPNLGSNVEA
                     IEVILKAIKAAGYKAGKDIWLALDCASSEYYKDKKYVLSAEAKPELTAQENVQFLADW
                     ARQYPIISIEDGMAEGDWAGWKQLTDALGATTQLVGDDLFVTNTHYLSRGIKEGIANS
                     ILVKVNQIGTLSETLDAVQMAQRAGYTAVISHRSGETEDSTIADLVVATNAGQIKTGS
                     ASRSDRVAKYNQLLRIEEELGEQAEYPGMDAFYNLAPKNKK"
     gene            3335..3637
                     /locus_tag="PKW60_10805"
     CDS             3335..3637
                     /locus_tag="PKW60_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPJ99965.1"
                     /translation="MLLVVAMAGMVIAYALHRDLFALYQSYRDRGAEVQASRDELRAA
                     QAFEEELSRKVEHLDGDAVEMEAAIRRNKNLVREGETIYRVELPPEIEPSGAPRSQ"
     gene            3760..>4095
                     /locus_tag="PKW60_10810"
     CDS             3760..>4095
                     /locus_tag="PKW60_10810"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPJ99966.1"
                     /translation="MRIVDRGIKVVVVGDDAETALVGQMLDEMLVAVRNGHTPSASDV
                     SYALSEARNRQLDKLGSVLGEIPAALRREFQIKPRTRGQRRYLDTITTHDLTLAIGPA
                     GTGKTYLAMA"
BASE COUNT          945 a         1213 c         1255 g          682 t
ORIGIN      
        1 gatgaaggaa gccgtatcga agacgccacc atcacggact cgatcatcgg catccgcacc
       61 attgtgggcc ggggcgtcgt catcaaccga agcatcatca tgggcgaaga ctttttcgag
      121 gaggagtgcc cacccggccg gatccccatc ggcatcggtg aaggcaccca tatcgaagga
      181 gccatcgtag acaaaaacac gcgcatcggc aggaacgtcc gaatctatgc ggccgacgcc
      241 ctgcccgatc agatcggcga cggctgggcg atccgcgacg gaatcgtcgt aatcctcaaa
      301 ggcgctgaaa tccccgacgg cacggtcatc ggaaaggcct gattcgcgac gcgggctgac
      361 cccacggcca tgctgaaggt tggcaggctt gatgccggtg ctataatcgc actcatgcac
      421 cacaacccgc gaaggagatg cgccatcatg ccggacaaaa agaaagacga ggcctccgat
      481 ttccgatacg aaacgggaga ggctcggata ccctgggctg ccgtcggcga acagatccgc
      541 gaagacgaca tcctggcatt gatcaagttt ctgctgcgcc ccgcccgagg ggaagagaag
      601 aaatacgacg cgcatttaaa gaaggtcgcc gctcagctcc gcgatctggg cgcggtgtcc
      661 acgccggcag gcaaactcag cctggcgggg aacgttcagg ccctggaaga agccaccgcg
      721 cgttttctgg gcgcgaaaca tgcggtgttt atcaccaatg ccacggccgg cttcgagatc
      781 gccttcaaat atgccggcct gaaacccggc gacgaggtca tcgcgccggc gctcaccttc
      841 attgccacca tcgcctatcc gctcgccatg ggcgcgaagg tggtgttgtc ggacgtcgaa
      901 ccgcgcagcc tcaacatgga tcctgccgat gtcgcccgca agattacgcc ccgtaccaag
      961 gcgatcatcc cggtgcacct cggcggttac cccgtcgaca tggcgccgct tatgaagctg
     1021 gcgcgtaagc acaacatcac ggtgattgaa gacgcggccc acgccttcgg cgcctcgtat
     1081 cgcggcaaga tgattggcac catcgggcac ttcgggtcgt tcagcttcca cgaggtcaag
     1141 aatgtcacct cgctcggtga aggcgggatt ctgacgtcga ccaccgcatt cggccgggaa
     1201 ctgcgccgcg cgcgcttcct cggcctcgat ttgacgcacc cgatccccaa ctggctgtac
     1261 gacgtgccgg cgttcgacgg aaaagacggc cccttcttca cgggcaacta ctccaccacg
     1321 gagatccagg cccttgggct gctgactcag atgaagcgcc tccagcgcat catcgcgaaa
     1381 cgaaagaagg ccgcccagta tctcgacaaa cgatttgcca aggtcgacgg cctcctgacc
     1441 ccgccgggtg atgacgcccg gatcaagagc acgtaccacc tgtatctgtt gcagatcgag
     1501 cccggcaaag tgggcggcga catccaggcc ctcaagaaaa aactcgacgc gcgcggcgtc
     1561 gtccaaatcc ctcatttcgc gccgttgtac aagttcagcg tcatgagaca gctcggttac
     1621 gacaccgcag ccatggaaaa gacctgtccg gtcgccgagg aggcgttcca gcaccgtttt
     1681 acccacctcc cgctatacga ttttaccagg gagcaattgg cgtatctcgc ggacgcagtg
     1741 atcgagtcga ttggcgagat gaagcggggc gtttaaccgg gcagcgcatc ggacggcttg
     1801 ccgagccgcc cgcagacaca aaaatggaaa taaccgcgcg gtacgcgttt gggtcaatgg
     1861 attttcttgc tgtcaatcga gtatcctata cggaattcga gatttcggaa gaggaaggag
     1921 aacgtagagc atgacacgga tcacagatgt acgggcgcgg gaaatactgg actcgcgcgg
     1981 caatcccacc gtggaggtgg aagtctacct ggccagcggc tttcgggggc gcgcggccgt
     2041 tccgagcggg gcgtcgaccg gcgaaaatga ggccctggag cttcgcgaca aagatcccaa
     2101 acggtatctc ggcaagggcg tcaaaaaagc cgtccagaac gtgaacgata ttattgccga
     2161 agagctgatg ggcatggaag ccaccgacca gcgcggcatc gacagcctgc tgatctcgct
     2221 ggacggcacc aaaaacaaag gcaaactggg cgcaaacgcc atgctgggcg tctcgctggc
     2281 cgccgcacgc gccgctgcgg aggcactcga agtgcccctc tacaaataca tcggcggcgt
     2341 gaacgcccac gtgcttccgg tcccgatgat gaacatcctc aacggcggac agcatgccga
     2401 caacaacgtc gacgtccagg aattcatggt catgcccgtg ggcgccaaga gcttcagcga
     2461 agccctgcgc atgggcgccg aggtgtttca cgccctcaag agcgtgctga aaaagcaggg
     2521 cttgaacacg gcggtgggcg atgaaggcgg atttgccccc aatctgggct cgaatgtcga
     2581 ggccatcgag gtcattttga aggccatcaa ggcggcaggc tacaaagcag gcaaagatat
     2641 ctggctggcc ttggactgcg ccagcagcga atactacaaa gataagaaat atgtgctcag
     2701 tgcggaggcc aaacccgaat tgaccgcgca ggaaaatgtg cagttcctgg cggattgggc
     2761 ccggcagtac cccattattt cgattgaaga cggcatggcg gagggcgact gggcgggctg
     2821 gaagcagctt accgacgccc tgggcgcgac aacccagctc gtgggcgacg atctgtttgt
     2881 cacgaatacg cactatctca gccgcggcat caaagaaggc atcgccaact ctatcctggt
     2941 gaaggtcaac cagatcggca ccttgtcgga gaccctggat gccgtgcaga tggcgcagcg
     3001 ggcagggtat accgcagtga tctcccatcg cagcggggaa accgaagact cgacgatcgc
     3061 cgacctggtg gtggcgacca atgcgggcca gatcaagacc gggtcggcgt cgcgcagtga
     3121 ccgggtcgcg aaatacaatc aactgctccg tattgaggaa gagctgggcg aacaggcgga
     3181 ataccccggg atggacgcct tctacaattt ggcccctaaa aacaagaaat agggtacaat
     3241 acggtacgta cccgaaaacc gggcgactct ctggacagag gagagttcat acagcgtata
     3301 gacgttaatg tcgcaacaag ctcgaaatcg ctacatgctt ttggtggtcg cgatggctgg
     3361 catggtgata gcctatgccc tccatcgtga cctttttgca ctttaccaaa gttaccgcga
     3421 ccgcggggcc gaggtgcagg cctcgcggga cgagctgcgc gcggcgcagg ctttcgaaga
     3481 agagttgtcg cgcaaagttg agcatctgga cggtgacgcg gtggaaatgg aagcagcgat
     3541 ccgccgaaac aagaatctgg tacgtgaagg cgagaccatt taccgggtgg agctgccgcc
     3601 ggaaatcgaa ccgagcggcg caccgcgttc tcagtagtcg gaccggtttg aggtaagact
     3661 gctctgacac acgagataca gctaaatagc caggaagaag ccgtaaagct tctgggacga
     3721 aacggcgaac tgcgcagacg gatccaggac gagaccgccg tgcgaattgt cgatcgcggg
     3781 atcaaggtcg tggtcgtcgg cgacgacgct gaaaccgcgc tggtcgggca gatgctggac
     3841 gagatgctgg tggcggtgcg caacggccac acccccagcg cgtctgacgt cagctatgcg
     3901 ctcagtgagg cgcgcaaccg ccaactggac aagctgggca gcgtgctggg cgaaatcccc
     3961 gcggcgttgc ggcgcgaatt ccagatcaaa ccgcgtacgc gcggacagcg gcgatacctg
     4021 gacaccatta caactcacga tctcacgctg gccatcggcc cggcaggcac aggcaagacc
     4081 tacctggcca tggcg
//
LOCUS       DAOPBQ010000342         4087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_17903_length_4087_cov_4.580605, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000342 DAOPBQ010000000
VERSION     DAOPBQ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17903_length_4087_cov_4.580605"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKW60_10815"
     CDS             complement(<1..315)
                     /locus_tag="PKW60_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99967.1"
                     /translation="MTVITGKPSPVSGNLAAVAGGAFLAVLAGFAVLAGGVYALLAEL
                     GLIVFVLILRWPVLGLYLTTALLLLSGPSGVIGSVRVAIPVTAAKVVGAMTFASWLLN
                     VTL"
     gene            complement(312..1571)
                     /locus_tag="PKW60_10820"
     CDS             complement(312..1571)
                     /locus_tag="PKW60_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99968.1"
                     /translation="MMRTAAAGLAFTVTALIGRQLGDALLGRLGLLLAVMDVAAGLVG
                     PALDTTLVRFAARHIAPGRDASLAYFQRMFRLKLAVAVGLLAAGAVLARPFLSYVMAP
                     SAGTAIGSGGVVLAFAGAAVLTLLGFAQAYYQAHFQMARYAFIELANSLLRLMVVGSL
                     LAVLGQPSALLLLGAFVASSALVAAGSLARLPGAVFHKATDEAVSLRAALGFAKWVAL
                     AAVATTLAQRADVFILGLTGTTGAAIGQYVAAFTVARLGDLAIMTLFSVLLPRTSALT
                     APDEFRRFLNRLLPLVLAAFAAGLPAFFASRWAVTLVFGASFAPAGLLCGILCLGVLA
                     SFGAAPAGAALYGMGRSRDVAALEVSKLIALAALGAFAARHFGVAGMAWTVSAVRATI
                     GIATYLCAYRGTRLFAQESVARGDHAL"
     gene            complement(1666..3087)
                     /locus_tag="PKW60_10825"
     CDS             complement(1666..3087)
                     /locus_tag="PKW60_10825"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPJ99969.1"
                     /translation="MAVRRQSRLFRLGVAFCDLVCAVCAYVGTLLLLQPEGQSLQDFA
                     RSNLAYFCVFALVWCGTATDQHLFALPREDNLKRLAWAVLKSVVVALVFSGFVITFFT
                     RQGVGREFFLTFGFLALTFILVFRLALRAILEYVRKQGFNFRRVVVVGANERAQKLVD
                     IIQSHLHYGYHIIGFVDDEPERQEAFKPHRLDYLGNCGELEQMLQEGVIDEVYICLPV
                     RSHYEKIRRIAYLCEGVGLPVRLVAELFPLRLARSHLAHFDDLPLLSLSTTPETYGQL
                     LMKRLIDFSASSLGLLATGWWLFPIVAILIKLESRGPVFFKQERVGQNGRRFHIYKFR
                     SMVVDAEERKKDLAALNEADGPVFKMRRDPRVTRVGSFLRKTSLDELPQLLNVWLGHM
                     SLVGPRPPIPSEVEQYSWEQRRRLSVRPGLTGLQQVSGRSDVSFDDWVAMDLAYIDEW
                     SLMTDLQILLLTIRVVLLGKGAR"
     gene            complement(3358..>4087)
                     /locus_tag="PKW60_10830"
     CDS             complement(3358..>4087)
                     /locus_tag="PKW60_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPJ99970.1"
                     /translation="AGVLTEASGWGAVGRYWHPATKGYRVSWGTLRPYMINTFFVCGA
                     TTLGVVALGSISAYILSRYRFYGSRIIFYFIIGTMMFPAALTLVPSFMVVRSLGLLNT
                     YGAMVLPYIAGGQVFATFVFKGFFDGLPEELFESARIDGAGHFRIYIDIVLPLSKPVL
                     SVVAIMTILGTWNNFLWPFIVNTDGRYHVIASGLYVLATTNFAQNYSSLFAAYMTASI
                     PLLLLFTYATKPFVQGVTSGAFKA"
BASE COUNT          906 a         1285 c         1263 g          633 t
ORIGIN      
        1 cagggtgaca ttgagcagcc agctggcgaa ggtcattgca ccaacgacct tggccgccgt
       61 aactggaatg gccacgcgaa ccgagccaat cacgcccgaa gggcccgaca acaggagcag
      121 cgcggtggtc agatacaggc ccagcacggg ccagcgaagt atcaatacga agacgatcag
      181 gcccagctcg gccagcaggg catagactcc ccccgccaga acggcaaatc ccgccagaac
      241 ggccaggaaa gcgccgcccg cgacagcagc gaggttccca gaaacaggcg acggcttgcc
      301 ggtgatgacg gtcacagcgc gtgatctccc ctggcgacgg attcttgtgc gaacagtctc
      361 gttccgcgat aagcgcagag atatgtcgca atccctattg tagcgcgaac tgccgagacc
      421 gtccatgcca ttccggcaac gccgaaatgc ctggccgcaa acgcgcccag cgcggcgagt
      481 gcgatcagtt tggacacttc gagggcggcg acatcgcgtg agcgccccat cccgtaaagc
      541 gctgcgcccg ccggggccgc gccgaacgag gccagcacgc ccaggcaaag aatcccgcac
      601 aggaggcccg cgggcgcgaa agatgcgcca aacacaagcg ttaccgccca gcgggaggca
      661 aagaaggcgg ggagaccggc agcgaacgct gccaaaacga gcggaagcaa gcggttcagg
      721 aaacggcgga attcgtccgg cgctgtcagc gcactcgtac ggggaagcag tacgctgaac
      781 agggtcatga tcgcgagatc gccgagccgc gctaccgtga atgcggccac atactgccca
      841 atggcggcgc cggttgtccc cgtcaggccc agaatgaaga cgtccgcccg ctgggcgagg
      901 gtggtggcaa cggcggccag cgccacccat ttcgcaaaac ccagcgctgc gcgcagtgag
      961 accgcctcat ccgtggcttt gtggaatacc gctcccggca gtcgtgccag actcccggca
     1021 gcgaccagcg ccgagctcgc tacgaacgcg ccgagcagga gcagggccga tggctgcccg
     1081 agcacggcaa gcaggctgcc cacaaccatc aggcgcagca ggctgttggc aagttcgatg
     1141 aacgcgtaac gcgccatctg aaaatgcgcc tggtaatatg cctgggcgaa ccccagcaga
     1201 gtgagcaccg ccgcgcctgc aaacgccagc acgaccccgc ccgagccaat ggccgtgccc
     1261 gcggacgggg ccatcacgta actcaagaag ggacgcgcca gcaccgcgcc ggccgcgagc
     1321 agccccaccg ctacggccag cttgagccgg aacatgcgct ggaaataggc gagcgaagca
     1381 tccctgccgg gggcaatgtg gcgcgcggca aaccggacca gcgtggtgtc gagggcgggt
     1441 cccaccaggc cggctgccac gtccatgacg gccagcagca gcccgaggcg gccgagcagg
     1501 gcatcgccga gctggcggcc gatcagcgcg gttacggtaa aggccagtcc tgccgctgcg
     1561 gtccgcatca agaggaccac cccgaaggcc tgcatccagg agcgcgtgcg gcggggcgcg
     1621 aaggcgtatg gggggttgtc ggccgtgccg ttcacagcgc gtgcgctacc gcgcgccttt
     1681 gcccagcaag accacccgga ttgtcaggag caggatttgg aggtcggtca tgagcgacca
     1741 ttcgtcgata taggcgaggt ccatggccac ccagtcgtcg aaactcacat cgcttcggcc
     1801 gctgacctgc tggagccccg tgagccccgg ccggacgctc aggcgccgcc gctgctccca
     1861 ggaatactgc tcaacttcgg acgggatcgg cgggcggggc cccaccaggc tcatgtggcc
     1921 cagccatacg ttgagcagct ggggcagctc gtcgaggctg gtttttcgga gaaacgagcc
     1981 cacgcgcgtg acccgggggt cccggcgcat tttgaagact ggtccgtcgg cctcattgag
     2041 cgcagccagg tctttcttgc gctcttcagc gtcgactacc atcgaacgga acttgtagat
     2101 atgaaaccgc cggccgttct ggccgacgcg ttcctgcttg aagaagaccg gcccgcgcga
     2161 ttccagcttg atcaggatcg ccacgatcgg aaacagccac catccggtgg ccagcaagcc
     2221 cagagacgat gcggaaaaat caatcagccg tttcatgagc agctggccgt acgtctccgg
     2281 tgtggtcgac agcgacaaca gcggcagatc atcgaagtgg gcgagatggc tgcgagccag
     2341 gcgcaagggg aacaattcgg ccacgaggcg caccggcagc ccgacacctt cgcacagata
     2401 cgcgatacgg cggatcttct cgtaatgcga acgaaccggc aggcagatgt agacctcgtc
     2461 aatcacgcct tcctggagca tctgttccag ttcgccgcaa ttcccgaggt agtcgagacg
     2521 gtgcggtttg aacgcctcct ggcgttccgg ttcgtcatcc acaaatccaa tgatgtggta
     2581 gccgtaatga aggtggctct gaatgatatc gaccagtttt tgtgcccgtt cgttggcgcc
     2641 gaccaccacc acccggcgga agttgaaccc ctgtttccgc acgtattcaa gaattgcccg
     2701 caaggccagc cggaacacca ggatgaacgt aagcgccagg aacccaaacg tcaggaaaaa
     2761 ctcgcggccg acgccctgcc gggtgaaaaa ggtgatcacg aatccgctga ataccagcgc
     2821 cacaaccacg gatttcagca ccgcccacgc cagtcgtttc aggttgtctt cccggggaag
     2881 agcgaataaa tgctgatccg tggccgtgcc acaccatacc agggcgaata cgcagaaata
     2941 cgcgagatta ctccgtgcaa agtcctgaag cgattgtcct tcgggttgaa gcagcagcag
     3001 cgtcccgaca taggcgcata ccgcacatac caggtcgcaa aacgccactc ccagcctgaa
     3061 cagcctggac tgtcgacgaa ctgccatctt tccccttcca gccccctgga tcgatgcttg
     3121 ggccgcgctt gtctccacga agggcacgta gtgagaattt tactgtattt tgccggaagt
     3181 gtcaagattc acccttccgg caatagccag ttaattggtt gtaaatcaat atgttacgac
     3241 aagcaaaccg ccttgaacag gctgcgcagt ttcgtggttg tggcaggccc cgccttctgc
     3301 gcgctgcaga gtcaacccgt tccgccgtgc catgatttcc tggagacgat cctccggcta
     3361 cgccttgaac gctcccgagg tcacgccttg cacgaaaggc ttcgtggcgt aggtgaagag
     3421 cagcaagagg ggaatgctgg cggtcatgta ggcggcgaac agactgctgt aattctgggc
     3481 gaaattggtt gttgccagca cgtagagccc cgaggcgatc acgtgatagc gcccgtcggt
     3541 atttacgatg aacggccaca ggaagttatt ccacgtgccg aggatggtca tgatcgccac
     3601 cacggagagc accggcttgg acagaggcag cacaatgtcg atatagatcc ggaaatggcc
     3661 tgcgccgtca atacgggccg attcaaacag ctcctcgggg agcccgtcga agaatccttt
     3721 aaacacaaag gtcgcgaaga cctgtccgcc cgcgatatag ggcaagacca tcgccccgta
     3781 ggtgttaagc aggccgaggc ttcgcacgac catgaacgag gggaccaggg tgagcgcagc
     3841 gggaaacatc atggtgccga tgatgaagta gaagatgatt cgcgaaccat agaaccggta
     3901 ccgcgaaaga atatacgccg agatcgagcc cagcgcgacc acgccgaggg tagtcgcgcc
     3961 gcacacgaag aatgtattga tcatgtacgg tctcagcgtg ccccaggaca cccggtaccc
     4021 tttggtagcc ggatgccagt agcggcccac ggcgccccat ccggaggctt cggtcaggac
     4081 gcccgcg
//
LOCUS       DAOPBQ010000343         4067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18021_length_4067_cov_3.370389, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000343 DAOPBQ010000000
VERSION     DAOPBQ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18021_length_4067_cov_3.370389"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..1475
                     /gene="leuB"
                     /locus_tag="PKW60_10835"
     CDS             378..1475
                     /gene="leuB"
                     /locus_tag="PKW60_10835"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019613462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPJ99971.1"
                     /translation="MTKTHTIAVLEGDGIGPETTAEAIKAIQAVSSRSGVQFDLRYAP
                     FGGQAYFETGASFPLETQKVCDEADAIIKGPIGLSHEESKKIPTQEQPERGALLPLRK
                     RYNTFANFRPVFLPPELIAFSPLKPEVVGDGINIMMIRELVSGLYFGAKENGTDANGL
                     RYVKETLEYNEQQIRDILHVAFKEARSRKKVLHNVHKSNVLISSVLWNTVLDEIAGDY
                     PDVTVKNILVDAAATAMVLHPRQFDVMVMENMFGDILTDLAGGLVGSLGLMPSACVGP
                     SKAYYEPSHGSAPDIAGKGIANPYSMIGSIALMLEKSFGMTEEAGWVWEALRAVFREG
                     CATPDLAKPELGYRALNTSEFGDMVARYIAA"
     gene            1537..2517
                     /locus_tag="PKW60_10840"
     CDS             1537..2517
                     /locus_tag="PKW60_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPJ99972.1"
                     /translation="MRDSSKTVAGLILFLAMVSWAQAPVFIRFLREAYEPFSMAFIRY
                     SSGALALTVVSLLFYRSEFLRLLRDPRPVLGIAVLNTFQQWTWTAGCYGSTATMAQVF
                     NMLSLVFVVIFSFMLFHEERAVIRSPLFAAGSSLCFLGAVAVLTKDPNSLRPVFDRSS
                     LLLLITAVSWGVYRVWSKHIVMTWHPIPMFTVLAWLTSAGFLALTFAVGRPSDLVNAG
                     GWLTFVALFSGIMPIAVAHPSFNYAQRNLGSAFCTSVGNLTPLLTYLFAIVMLNDEFL
                     RLSQWLGAFVLIGGAFLVNLAGRRVQNGQAAADMPPVEAATADSGPGRAR"
     gene            complement(2483..>4067)
                     /locus_tag="PKW60_10845"
     CDS             complement(2483..>4067)
                     /locus_tag="PKW60_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99973.1"
                     /translation="GQWGVGPTSAWYSDAHLLMPGVFGYGATNAAMPPALYEAMIFNG
                     AMTGATVYVFEPEDDLWFGAARHHWKNAIYPALRELTDYALIPRKEFVEEKVRVAFQL
                     GRCRSPLDFQHNLRDIDGVSGEGYLIWGAYGMERPGQIEELIPNSGRHYWIPILSPYA
                     SQRALQQFAEVVQPGRMASPQAWTDLLDKYYVPDGSGTASIYRVGRAVYILNNRESHR
                     QVQDFRVPGVPAPVWKVQARRTQEGIELTWPFREYDFAYKVYRRYGPTEPFQLVAGDV
                     EGRRWLDALPEVGQSVAYAVTALTTENEVFTGNVDFGECLALSVVESRIAEEVVVTPL
                     ADVFDSRPLPRSGALTDGIEAWWPGFEGVPNERMAVAQSIVERIERLDRAFSEKDLDG
                     VLDLYADEYVDPENWGLQYVRRAFQWFFERYNRCSMARQIRRWDFSAYEPGQEVRVLL
                     FCRFNGIAVSDFTGRFAGEEAYFPRTERGEVWLTFANYDGHWRIRGSQPALPNFKDIL
                     SFSGSPYEAIGPGPDRYQP"
BASE COUNT          804 a         1327 c         1139 g          797 t
ORIGIN      
        1 cgataccggc tcttgtgtcc cccgctggcg ggggtggaca cgcgtcccgc gtggccgggg
       61 gtggactgcg tggatcccgc gtgggcgtcc tgaagaaggg caggcacagg ggcctgcctc
      121 tacaaacgcg gtacgccttg ccggaggcgg accatgccgt gccagcgctg ccgcttccgg
      181 ctcttgtgtc ccacgctgac gggagtggcc gcgaagcggc cgggggtgga ctcccatcac
      241 cggtacgatc gcagatcggc gcaagccgga agtggaagtg cgcggcgcca acgtgctagt
      301 ctttcagctc tgcttccaat ccgccggatt ctatacgccc tgcggattaa gtcacgaccg
      361 cgtatcccgg agacaacatg acaaaaacac ataccattgc tgtgctggaa ggtgacggca
      421 tcggacccga aaccacggcc gaggctatca aagccattca ggcggtcagc tcacggagtg
      481 gcgtccagtt tgacctccgt tacgcgccgt tcgggggcca ggcctatttt gaaaccggcg
      541 cctcatttcc actcgaaaca cagaaggtct gcgacgaagc tgatgccatc atcaagggac
      601 ccatcgggct gagtcatgag gaatcaaaga aaatcccgac gcaggagcag cccgaacgcg
      661 gcgctcttct gcccctgcgt aaacgctaca acactttcgc caacttccgg cccgttttcc
      721 ttcctccgga gctcattgcg ttttccccgc tcaaacccga agtggtaggc gacggaatca
      781 acatcatgat gatacgggag ttggtcagcg ggctctattt cggcgcaaag gaaaatggaa
      841 ccgacgcaaa cggcctccgc tatgttaaag aaaccctgga atacaacgag cagcaaatcc
      901 gcgatatcct gcacgttgcg ttcaaggaag cgcgaagccg aaagaaagtg ctccacaacg
      961 tccataaaag caatgtgctc atctccagtg tattgtggaa taccgtcctg gacgagatcg
     1021 cgggcgacta tcccgatgtc accgtaaaaa acatcctggt cgacgccgcc gccaccgcca
     1081 tggtgctgca tccccgccag ttcgatgtca tggtcatgga aaacatgttc ggtgacatcc
     1141 tgaccgacct cgcaggcggg ttggtgggct ccctgggact catgccctcc gcctgtgtcg
     1201 gccccagcaa ggcctattac gaaccgtccc acggatccgc cccggatatc gcgggaaaag
     1261 gcattgcaaa cccctattcc atgatcggat ccatcgccct catgctcgag aagagctttg
     1321 gcatgacgga agaagccggg tgggtctggg aggccttgcg ggcggtgttc cgtgaaggct
     1381 gcgcgacgcc cgacctcgcc aaacccgagc tgggttaccg ggcactgaac accagcgaat
     1441 tcggcgacat ggtcgcccgc tatatcgccg cctgaacatc tcagcggcat ggaaacccga
     1501 acgcaacagc ggacaggacc tcagagcacc ggaccaatgc gggacagctc caaaaccgtc
     1561 gccgggttga ttctgtttct cgccatggtc agctgggcgc aggccccggt atttattcgt
     1621 ttcctgcgcg aggcctacga gccgttttcc atggccttca tccggtactc gagcggcgcg
     1681 cttgcgctga ccgtcgtcag tttgctcttc taccggagtg aattcctgcg cctgctccgc
     1741 gacccgcggc ctgtactcgg tatcgctgtg ctcaatacct tccagcaatg gacctggacc
     1801 gcgggatgct atggctccac tgccacgatg gcgcaggtgt tcaacatgct ctccctggtc
     1861 tttgtggtga ttttcagctt catgcttttc cacgaagaac gcgcggtcat ccgcagtccg
     1921 cttttcgccg ccggttcctc cctgtgcttt ctgggggcag tggcggtgct cacgaaagat
     1981 cccaactcgc tccggcccgt cttcgaccgt tcgtccctgc tgctgctcat cactgccgta
     2041 agctgggggg tataccgggt ctggagcaag cacattgtca tgacctggca ccccatcccc
     2101 atgttcacgg tactggcatg gctcacttcc gcaggatttc ttgcgctcac attcgccgta
     2161 gggcgcccat cggatcttgt gaacgcgggc ggctggctca ccttcgtcgc gttgttttcg
     2221 ggcatcatgc ccatcgcggt tgcccacccc agcttcaatt atgctcagcg aaacctcggc
     2281 tcagcgttct gcaccagtgt agggaatctc accccgctgc tgacctatct cttcgccatt
     2341 gtgatgctca acgacgaatt tctccgtctg tcgcagtggc tgggcgcgtt tgtcttgatc
     2401 ggcggagcat ttttagtgaa cctggcgggg cgcagggtgc agaacgggca ggcggcggca
     2461 gatatgccgc ccgttgaagc cgctacggct gatagcggtc cgggccgggc ccgatagcct
     2521 catacgggct gccggaaaag ctgaggatgt ctttgaaatt cggcagtgcc ggctgcgaac
     2581 cccggatccg ccagtggcca tcgtaattcg caaacgtaag ccagacctcg ccccgttcgg
     2641 ttcgtggaaa ataggcttcc tcacccgcaa accggcccgt aaaatccgac acggcgatcc
     2701 cgttgaaccg gcagaacaac agcacccgca cttcctgccc cggttcatag gcggagaagt
     2761 cccagcgccg gatttggcga gccatggagc accggttata gcgttcaaaa aaccattgaa
     2821 acgcccgccg gacatattga aggccccaat tctccggatc cacatattca tcggcgtaga
     2881 gatccagcac gccgtccaga tccttttctg aaaaggcccg atccagcctc tcaatgcgct
     2941 cgacaatact ctgggccacg gccattctct cgttcgggac cccttcaaat cccggccacc
     3001 acgcttcaat cccgtcggtc aacgcaccgc ttcggggcag aggccggctg tcaaagacgt
     3061 cagccagcgg cgtcaccacc acttcctcgg cgatccggct ctcgaccacg ctcaaggcca
     3121 gacactcgcc gaaatccacg ttacccgtga agacctcgtt ctcggtggtc agcgcagtga
     3181 ccgcataggc tacgctttgc cccacttccg gcagggcatc cagccagcgc cgcccctcga
     3241 catctcccgc caccagctga aacggttccg tgggaccata ccgccggtac accttatagg
     3301 caaagtcgta ttcgcgaaat ggccaggtca gctcgatgcc ttcctgcgtc cggcgcgcct
     3361 gcaccttcca gacaggcgcc ggtacgcccg gcacgcgaaa gtcctgcacc tgccggtggc
     3421 tctcgcggtt gttcaggata taaacggcac gcccaacccg atagatagag gcggtcccgg
     3481 aaccgtccgg cacatagtat ttatccagaa ggtccgtcca ggcctgcggg gacgccatcc
     3541 tgcccggctg gaccacttcc gcaaactgtt gaagggcgcg ctgtgacgcg tagggcgaca
     3601 gaattggaat ccagtaatgc cggccggaat tggggatcaa ctcttcgatc tggcccgggc
     3661 gctccatccc gtaggccccc cagatcaggt agccttcccc ggacactccg tcgatatcgc
     3721 gcaaattgtg ctgaaaatcg agcggcgaac ggcaccggcc cagctgaaat gcgacgcgca
     3781 ccttctcctc cacaaattct ttgcgcggaa tcagggcgta atccgtcaac tccctcaatg
     3841 ccggatagat tgcgttcttc caatggtgtc gtgccgcccc gaaccacagg tcgtcttccg
     3901 gctcaaagac gtacacggtt gcgccggtca tcgcaccgtt gaagatcatg gcctcgtaca
     3961 acgccggcgg catcgcggca ttcgtagctc cgtagccgaa gacgcccggc atcagcaagt
     4021 gcgcatcgct gtaccacgct gacgtcggcc cgactcccca ctggccg
//
LOCUS       DAOPBQ010000344         4059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18060_length_4059_cov_4.104146, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000344 DAOPBQ010000000
VERSION     DAOPBQ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18060_length_4059_cov_4.104146"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PKW60_10850"
     CDS             <1..642
                     /locus_tag="PKW60_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99974.1"
                     /translation="PHPHGLAGIYADVKAAFWAAIQAGNTPGLRTEGTGTTSADCPLT
                     AVGNVEYNGSNPPKYLDGFFDTVEIRDAAGNWQPVQDGGAVALGGDQPLVARLGMTNL
                     GEAAWLPPTEAGDAGAVYVRVDAVTTDWGPSSRIAPGIYPGESRFPIPVRVDRFGQSV
                     LEEVTLMSDFPPLARLTLEFRFEAEGRAVFGPRYTVEVHSYPLADEIVRGEIP"
     gene            639..3047
                     /locus_tag="PKW60_10855"
     CDS             639..3047
                     /locus_tag="PKW60_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99975.1"
                     /translation="MRTLAVAGVALLLVFAASAQSTSPAENIALGKSYTMQPRPNYEH
                     CTDPGDATQLTDGVYTEGYFWAQKTTVGWTHQPIIVVTIDLGEDLPISGASFNTAAGT
                     AGVAWPAAIYVLVAGEDKIFYEAGELISMSSGHGLPDPARYSVHRYWTDQLKTHGRYM
                     AFAFDAQPYGFVDEIEVYRGEDEWLQYAYTGTPIKDVKDAVVNASLQQAIRRRILLDI
                     AGIEEKLPKAAIDAGAKEAIAAGLTEARSACVELPEADLETFKAVIPLNDLHRDVLAL
                     QGALWQAQGLAPLTAWQPPLWDQAGLLDDPVAGASPKVAVHLMRNEYRAGCFSLSNAT
                     GEPDTVRVTFTGLPGGDNPGYITVHEVQWTDTKAGIPVAAALPEAPRLGGSSVIEIPA
                     GMTRQVWFTFHPQSVDPGTHTGEIVMASARHGELRVPVEMRVYPFEFPARPTLHFGGW
                     DYSNGPGNRGVTPSNMAQLIAHCREHYVDSPWATNAVMPWGEYDAAGAMTAAPSTAAF
                     DEWMDRWPDAANYMVFSAVGSKMAGFAQGTPEFNRAVGEWTVFWAEYMKGKGKQPEQL
                     GLLIIDEPHRAEQDETILHWALAIRAMDTGIRIWEDPIYRGDMTQANPEMIAACHVLC
                     PNRPLFLNGPDAYRQYFVDRRNEGITLEFYSCSGPVRLLDPYGYHRMQAWECWAYEAV
                     GTYFWALSDTGNGSSWNEYIAPGTDYCPFFLDDDTVTAGKHMEAIRESIQDFEYLVML
                     RDAIVRAGESDTAAAAGALLAELPGRVLDASRSKTQYWNEPLDRAVADRARLEILDML
                     VQLK"
     gene            3070..>4059
                     /locus_tag="PKW60_10860"
     CDS             3070..>4059
                     /locus_tag="PKW60_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HPJ99976.1"
                     /translation="MYSLTLVIAAGLSAAGAEAVPFYADKLDLTYYLDASGARHPIAS
                     VQDWEIRREHVRAGLQQVAGPLPSRETLAPLDLQVEQEDDFPAYVRRKITFAVEPWDR
                     LPAYLLIPKGKEGPLPAVLCLHPTADIGKGVVVGLGGRENRQYAEELAAMGFVTLAPD
                     YPGFGDYKDARQRAYARGYTSMTMKGIWNHMRCVDLLQSLPEVDANRIGCIGHSLGGH
                     NTLFVGLFDERVRVMVTSCGFTLFAKYKDGDLTGWTHDGYMPAIAETYGKDPAAMPFD
                     FPDILAALAPRTVFINAPVNDDNFDVSGVQDCVAAAQPVFALYGANDRLIAVYP"
BASE COUNT          744 a         1311 c         1271 g          733 t
ORIGIN      
        1 ccgcatcccc acgggctcgc cggcatttat gctgacgtga aagccgcctt ctgggcggcc
       61 atccaggcgg gcaacacccc cgggctccgc accgagggca ccggaaccac ctccgcggat
      121 tgcccgctca ccgccgtggg caatgtggag tacaacggca gcaatccgcc caaatacctc
      181 gatggatttt tcgatactgt cgaaattcgt gacgccgcgg gcaactggca accggtgcag
      241 gatggcggcg cggtggccct gggtggggac cagccgttgg tcgcgcggct gggcatgacc
      301 aatctcggcg aagccgcctg gctgccgccg acggaagctg gagacgctgg cgccgtctac
      361 gtgcgcgtgg acgcggtgac gacggactgg ggaccgtcct cacgcatcgc tcccggcata
      421 tatccgggcg agtcgcgttt tcccatcccg gtgcgcgtcg accggtttgg acagtccgtg
      481 ctcgaagagg ttaccctgat gtctgacttt ccgccgttgg cgcggctcac cctcgagttt
      541 cgctttgaag ccgaaggtcg cgccgtgttc ggcccgcggt atacggtgga ggtgcattcg
      601 tatccattag ccgacgagat agtcagagga gaaataccat gagaacactt gctgttgctg
      661 gagtcgcgct gctgctcgtt ttcgccgcct ctgcgcaaag taccagtccc gccgagaaca
      721 tcgcgctggg caaatcctat acgatgcagc cccggcccaa ttacgagcac tgtactgacc
      781 cgggcgatgc cacacagctt accgatggcg tgtatacgga aggctatttc tgggctcaga
      841 agaccacggt cggctggacc catcagccga tcatcgtcgt gaccattgat ctgggagaag
      901 atctgccgat cagcggagcc tcgttcaaca ccgccgctgg cacggccggg gtcgcctggc
      961 ccgcagcaat ctacgtgctc gtggccggtg aagacaagat cttctacgag gcgggcgaac
     1021 tcatctcgat gagctccggc cacggccttc ccgatccggc caggtattcc gtgcaccgct
     1081 actggaccga ccagctcaag acgcatgggc gatacatggc cttcgccttc gacgcccagc
     1141 cgtacgggtt cgtcgacgag atcgaagtgt accggggtga ggacgaatgg ctccagtacg
     1201 cctacacagg aactcccatt aaagacgtca aggacgctgt ggtcaacgcg tcgctccagc
     1261 aggcgatccg ccgccgcatt ctcctcgaca tcgcgggcat tgaagagaag cttccgaaag
     1321 ccgccatcga cgcgggcgcg aaggaggcca tcgcggctgg actgacggag gcccgcagtg
     1381 cgtgcgtgga gctgcccgag gccgatctgg aaacattcaa ggccgtcata ccgctcaacg
     1441 acctgcaccg cgacgtactg gcgctccagg gcgccctgtg gcaggcgcag gggctcgcgc
     1501 cgctcaccgc ctggcagccg ccgttgtggg accaggccgg cctcctcgac gacccggtcg
     1561 cgggcgcatc tccgaaggtt gcggtgcatc tcatgcgcaa tgagtaccgg gccggctgtt
     1621 tcagcctttc caacgccacc ggggagcctg acaccgtgcg ggtcaccttt accggtcttc
     1681 ccggcgggga caaccccggc tacattaccg tgcacgaagt ccaatggacc gacaccaagg
     1741 cggggatccc cgtcgcggcg gccttgcctg aggcgccccg gctgggcggc tccagcgtga
     1801 tcgagattcc ggccggcatg acgcggcagg tctggttcac cttccatccc cagtccgtcg
     1861 acccaggcac gcataccggc gagatcgtga tggcatcggc acgacacggt gaactgcggg
     1921 ttccggtgga gatgcgcgtc tacccgttcg aatttcccgc gcggccaaca ctgcatttcg
     1981 ggggctggga ttacagcaac ggccccggca acagaggcgt cacgcccagc aacatggctc
     2041 agcttattgc ccattgccgc gaacattatg tggattcacc ctgggccacc aacgccgtga
     2101 tgccctgggg cgagtacgat gcggcggggg cgatgaccgc tgcccccagc acggctgcgt
     2161 tcgatgagtg gatggaccgc tggcccgatg ccgccaatta catggtgttt tcggcggtcg
     2221 gctcgaagat ggccggattt gcgcaaggca cgcccgagtt caaccgtgct gtcggcgaat
     2281 ggaccgtgtt ctgggcggaa tacatgaaag gcaagggcaa gcagccggaa cagctcgggt
     2341 tgttgatcat cgatgagccc caccgcgccg aacaggacga aaccatcctc cactgggccc
     2401 tggccatccg cgccatggat accggcatcc gcatctggga ggatccgatc taccggggcg
     2461 acatgacgca ggcaaacccg gagatgatcg ccgcctgcca tgtcctctgt cccaaccggc
     2521 ccctgttcct caacggcccg gacgcgtatc gccagtattt tgtggatcgg cgcaatgagg
     2581 gcatcacgct cgagttctat tcatgcagcg gccccgtgcg cctgctcgac ccctatggct
     2641 atcaccgcat gcaggcttgg gaatgctggg cgtatgaggc cgtgggcacg tatttctggg
     2701 ccctgagcga tacgggcaac ggctcgtcgt ggaacgaata catcgcgccc ggcaccgact
     2761 actgcccgtt cttcctggac gacgatacgg tcaccgcggg taaacacatg gaagccatcc
     2821 gcgagagcat ccaggacttt gaatacctgg tcatgctccg cgatgcgatt gtccgggccg
     2881 gagaatccga tacagcggcc gcggcaggcg cgctcctcgc cgagctgccc ggccgggtgc
     2941 tcgacgcgag ccgcagtaag acccagtact ggaatgaacc gctcgatcgg gctgttgcgg
     3001 accgcgcacg gctcgagatt cttgatatgc tggtgcagct gaagtaaacc gcggcaagga
     3061 gactgcgtta tgtattcact gaccctggtc atcgcggcgg gactgtctgc ggccggcgcg
     3121 gaagcggtcc cgttttacgc cgacaaactc gatttgacgt actatctcga cgcatccggc
     3181 gcgcgccatc ccatcgcatc cgtccaggac tgggaaattc ggcgtgagca tgtccgcgcc
     3241 ggtctccagc aggtggccgg gccactgccc tctcgcgaga cgctggcccc cctggatctg
     3301 caggtcgaac aggaggacga ttttcccgcc tatgtccgca ggaagatcac gttcgcggtg
     3361 gaaccgtggg atcgtctgcc cgcgtatctc ctgatcccga aagggaaaga aggtcccctg
     3421 cccgcggtcc tgtgcctgca ccccaccgcc gatatcggca aaggcgttgt cgtgggtttg
     3481 ggcggcaggg aaaaccggca atacgccgaa gaactggccg cgatggggtt cgtgaccctt
     3541 gcccccgact accccgggtt cggcgattac aaagacgccc ggcagcgcgc gtatgcccgc
     3601 ggatatacca gcatgaccat gaaaggcatc tggaaccata tgcgctgcgt cgatctgctc
     3661 cagagcctgc ccgaggttga tgccaaccgc atcggctgca tcggccattc cctcggcggt
     3721 cataacacgc tctttgtagg cctgtttgat gagcgggtcc gcgtgatggt cacgagctgc
     3781 ggattcaccc ttttcgcgaa gtacaaagac ggcgacctca caggatggac gcatgacggc
     3841 tacatgcccg cgattgccga gacctacggc aaggatccgg cagcgatgcc attcgatttc
     3901 ccggatattc tcgccgcgct tgcgccgcgt accgtgttca tcaatgcgcc cgtgaacgac
     3961 gacaacttcg atgtctcggg ggtgcaggat tgcgtggcgg ccgcgcagcc cgtgttcgcg
     4021 ttgtacggcg caaacgaccg cctgatcgcc gtgtatcct
//
LOCUS       DAOPBQ010000345         4055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18076_length_4055_cov_4.535500, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000345 DAOPBQ010000000
VERSION     DAOPBQ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18076_length_4055_cov_4.535500"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2741
                     /locus_tag="PKW60_10865"
     CDS             <1..2741
                     /locus_tag="PKW60_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPJ99977.1"
                     /translation="PSEQSLCFSYWSDADPYTVKDDGIQVTSQWRLAPGVEAAIGQMS
                     IWLGRPGPSGFLIEAERWFRAHGFAQPLEYPGWLARGVLYEASAGGHVDSGFSDVGGF
                     DAFSRQVDYLADLGVNVLWLNAVHEHKRPPNPTDGAWNHYDPLNFDVVDPILGGPDAL
                     ARLADALKSAGIHFLCETVPHGGHSIQAEALEAWWTRDREGRPRNPWGGYGMDNASPE
                     WQDVIRGHMAMLAREFGIEGARIDVADGQGPNWGSPRTPHASYSTVGGGLEMMRAIRD
                     GIAQGPASRPVLIPESPLRREYFSISDAYLVGYGFEFVDFIRDHTGLARAEPANMAAR
                     LHAFFEAERGAMPPGALLIRTLNNHDTVVERGRVQNRFGAGLTRALYGVCLAVPGLPM
                     MYQEEETGNFEALRAMNWTRRLLPELGVGEAEFLPPGSFAPEVFAVMRRDAQHQTLVL
                     ANLSGRPVSGRAPLPGIPDETPVYDAVAAVMSGKGKGEPAAHVRDRGVQWTLSPYETA
                     FLRFGTPPDASIPAARFATFAANEPDASDTLVWQAGSEGLALHCGAIEASLTLPVAAW
                     ETRGDGDGVTRLISTAGTIECRESEGGVAVLCEINAEYAHTPLTLRVSNARGWRVSGE
                     SAVLSDWFVPRHHPFPEGSDYVWRPSDVWGYLPYNLYNAMLPVGRLWQSLLEPLLAEA
                     PAVAFLSENGQGLALRHIETSAMNVVLHDGGMAHDTTAAQPALEFFAIDTNLHLQVQH
                     LGQRQPWLLSGLQPGEPRPLRVAFTLTPFGDAAGDFAAERAPRAARGPVLEREGPEFV
                     DSFAAVFMPRPAALTWRNLAALPGVYRLELELRHSERGPDGRDLTEAYTIELDGTPVS
                     LDWVKLNTTSTGNAWFGHAQTAPIELAEGPHTLSIRTSRPWCAVRNGFRLIASPTD"
     gene            2945..>4055
                     /locus_tag="PKW60_10870"
     CDS             2945..>4055
                     /locus_tag="PKW60_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPJ99978.1"
                     /translation="MQVHGGGMYESFYGLREKPFNLTPDPRFLYLSEMHKEAFAHLIF
                     GIRNRVGFIMVTGEIGTGKTTICRSLLGQLDQDVELAFIFNPCLSPEELLRKINEDFG
                     IRSRAISVKGLIDELNTYLLDRHANNKNCVLVIDEAQNLTPPVLEQIRLLSNLETERQ
                     KLLQIVLIGQPELAEHLELPELRQLNQRITARYHLKPLGEKETLQYIAYRLRVAGGMR
                     KVRFTRGAIKTVYRHSKGTPRVINAVCDRALLIGYSKETQEITRSLVKQAAREIRGER
                     IRTKKKGALKRYIPTPRLMAAAALIVLVGLFIVRPVAKRLDVLVALLQNPPANGPALA
                     GAPALEPGGTHAGNTHGDGNGIVDGTTGWSLDHFGT"
BASE COUNT          729 a         1284 c         1309 g          733 t
ORIGIN      
        1 cgccgtcaga gcagagcctg tgcttttcgt actggagcga cgcggatccg tacacggtca
       61 aagacgacgg catccaggtc acgagccagt ggcgcctggc gccgggcgtg gaggcggcca
      121 tcggtcagat gagcatctgg cttggacgcc ccggcccatc gggatttctc atcgaggcgg
      181 agcgctggtt ccgtgcgcac ggattcgcgc aaccgctcga gtaccccggc tggctcgcgc
      241 ggggcgttct gtatgaggct tcggctgggg gccatgtcga ttccgggttc agtgatgtgg
      301 gcgggtttga cgcgttcagc cggcaggtcg attacctggc cgacctcggc gtaaatgtcc
      361 tctggctgaa cgcggtacat gaacacaaac gcccgcccaa ccccaccgac ggcgcctgga
      421 atcattacga tccgctgaat tttgatgtgg tggatcccat tctcggcgga ccggacgcgc
      481 tcgcccggct cgcggatgcg ctcaaaagcg cgggcatcca tttcctgtgc gaaaccgttc
      541 cgcacggcgg ccacagcata caggccgaag ccctcgaggc ctggtggacg cgcgaccggg
      601 aaggccggcc ccggaacccc tggggcggct acggcatgga caacgcctca cccgaatggc
      661 aggacgtcat ccgcggccat atggccatgc tcgcgcgcga attcggcatc gaaggcgccc
      721 ggattgacgt cgccgacgga cagggcccga actggggctc cccgcggacg ccccatgcct
      781 cctattccac cgtcggcggt gggctcgaaa tgatgcgggc catccgcgac ggcatcgcgc
      841 aggggccggc atcccggccc gtgctgatcc ccgaaagccc gttacggcgc gaatatttct
      901 cgatatccga tgcgtacctc gtcgggtatg gatttgagtt cgtcgatttc atccgcgacc
      961 acaccggcct ggcccgggct gaacctgcga acatggctgc gcgactccat gcctttttcg
     1021 aggccgaacg gggcgcgatg ccgcccggag ccttgctgat ccgaacgctc aacaatcacg
     1081 atacggtggt tgagcggggc cgggtgcaga accgcttcgg cgcaggcctc acgcgggcgc
     1141 tgtacggcgt ctgcctggcg gtgcccggcc tgccgatgat gtatcaggaa gaagagacgg
     1201 gaaatttcga ggcgctgcgc gccatgaact ggacccggcg tctgctcccc gaactgggtg
     1261 tgggcgaggc cgaatttctg ccacccggct cgtttgcgcc ggaggtcttc gcagtgatgc
     1321 gccgggatgc gcagcatcag accctggtcc tcgccaacct ttccggccgc ccggtttcgg
     1381 gacgagcccc cttgcccggc atcccggatg agacccccgt gtacgatgct gtcgccgcgg
     1441 tgatgtctgg caagggcaag ggcgagcctg ccgcacacgt gcgcgaccgt ggtgtgcaat
     1501 ggaccctctc gccgtatgaa acagctttcc tgcgcttcgg cacgccgccg gacgcgtcca
     1561 ttccggccgc acggttcgcg acgtttgccg cgaatgagcc cgatgcctcc gatacgctcg
     1621 tctggcaggc ggggagcgag gggttggcgc tgcattgcgg cgccatcgag gcctccttga
     1681 ccctgcccgt cgcagcctgg gaaacccgcg gcgatggcga cggggtcact cggctgatct
     1741 cgaccgcggg caccatagag tgccgcgaaa gtgagggcgg cgtcgcggtt ctctgcgaga
     1801 tcaacgcgga atacgcgcac acgccgctca cgctgcgggt ctcgaatgca cggggctgga
     1861 gagtctcggg cgaaagcgcg gtcctttcag actggtttgt gccgcgtcac catccttttc
     1921 ccgagggcag cgactatgtg tggcggcctt ccgatgtctg ggggtacctg ccgtacaacc
     1981 tctacaacgc tatgctcccg gtgggccggc tctggcagag cctgctcgaa ccgctgctcg
     2041 ccgaagcacc cgctgtggcg tttttaagcg aaaacgggca gggtctggcg cttcggcaca
     2101 ttgaaaccag cgcgatgaat gtggtgttgc acgatggcgg catggcacac gatacgaccg
     2161 cggcgcaacc ggcattggaa ttcttcgcca ttgacacaaa tctccatctg caagtgcagc
     2221 atctggggca acggcagccg tggctgttga gcggacttca gcccggggaa ccccgccctc
     2281 tgcgcgtggc gttcaccctg actccgttcg gcgatgccgc cggggatttc gcggcggagc
     2341 gcgcgccacg cgcagcgcga ggaccggttc tcgagcgcga aggcccggag ttcgttgatt
     2401 ctttcgcggc tgtgttcatg cccaggccgg cggcgcttac gtggcgcaac ctcgcggcgc
     2461 ttcccggcgt ctaccgcctg gaactcgagc tgcgccacag cgaacgcgga cccgacggcc
     2521 gcgatttgac cgaggcctac acaatcgagc tcgatggtac gcccgtgtct ctcgattggg
     2581 tcaaactgaa cacgacctcc accgggaatg cctggtttgg ccacgcgcag acggctccaa
     2641 tcgagctcgc agagggtccg cacaccctca gcatccggac cagccggccc tggtgcgcag
     2701 tccgcaacgg atttcgtctg atcgcgtctc cgacggacta aaccggcacc ggcggcggcc
     2761 cggcgagatt tcacaagact tgtcagtcct gtgaattctg tgctgcggtt tcgtaccccc
     2821 tcataaaggc gggcaacagc ctccggcagg ccttcgcttc gatataaatc agcggattgc
     2881 cggggggtta cgttgactgt gcgggggcag catgctatgc tgatttgtca agaattccct
     2941 gaaaatgcag gtccatgggg gcggcatgta cgagagcttt tacgggctgc gcgaaaaacc
     3001 cttcaatctg accccggacc cccggttttt gtatctcagc gagatgcaca aagaggcgtt
     3061 tgcgcatctg atcttcggga tccggaaccg cgtcgggttc atcatggtga cgggagagat
     3121 cgggaccggt aagaccacga tctgccggtc gctcctgggc cagctggatc aagacgtcga
     3181 actggcgttc attttcaacc cgtgcctttc gccggaagag ctgcttcgga agatcaacga
     3241 ggattttggc attcgctcgc gggcgatttc ggtcaaaggc ctgatcgacg agctgaacac
     3301 gtacctcctc gaccggcatg caaacaataa gaactgcgtg cttgtgatcg atgaagcgca
     3361 gaacctgacg ccgcccgtgc tggaacaaat ccggctcttg tcgaatctgg agacggaacg
     3421 ccagaaattg ctccagatcg tgctcattgg ccagccggag ctcgcggaac acctcgagct
     3481 gccggagctg cggcagctga accagcggat cacggcccga tatcacctca aacccctggg
     3541 cgaaaaggag acgctgcaat acatcgcgta ccggctccgt gtggcgggag gcatgcgcaa
     3601 ggtacggttc acgcgggggg cgatcaaaac cgtttaccgg cattccaaag gcacgccgcg
     3661 cgtgatcaat gccgtttgtg accgggcgct gctcatcgga tattcgaaag aaacgcagga
     3721 aatcacccgc tctctggtga aacaggcggc gcgtgaaatt cgcggcgagc ggatacggac
     3781 aaagaagaaa ggcgcgctga aacgctacat tccgacgccg cgcctgatgg ctgcggctgc
     3841 gctgatcgtg ctggtgggct tgtttatcgt ccggcccgtg gcaaaacggc tggatgtgct
     3901 cgtagcgctc ctgcagaacc cgccggccaa cggccccgcg ctcgcgggag cgccggcgct
     3961 cgaacctggc ggcactcatg ctggcaacac ccatggtgac ggcaatggca tagtagatgg
     4021 caccaccgga tggagccttg accacttcgg cacca
//
LOCUS       DAOPBQ010000346         4022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18257_length_4022_cov_3.821528, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000346 DAOPBQ010000000
VERSION     DAOPBQ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18257_length_4022_cov_3.821528"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="PKW60_10875"
     CDS             <1..1073
                     /locus_tag="PKW60_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPJ99979.1"
                     /translation="KVDRTRKQIESNDAFAKKRDIKDDHCFTGLDAYKGILKTDIDIL
                     LEGSLPYCRPMHLEAAVEAKKHIFTEKPVAVDPVGIHQVIRAAKRADELGLSFVAGTQ
                     RRHQRPYRETIAKIHEGAIGDIVSLRAYWCGGLPFAHERKPEWTSDLEYCIRNWYAHC
                     WVCGDNIVEQHVHNLDVCNWVMEGFGIEHPISVFASGGRQFKPNEPKYGDLWDHFSCD
                     FEYPNDVHMTSFSRHWDGLDDGIFEAAYGTKGISNCRDMGEYGPDPYVQEHIDLVNSI
                     TGEGRHYNEGVQVAYSTMTAIMGRISAYNGRRVTWEDVMEGKLKDYSIVPELDWDRAY
                     PNEPVPVPGKVGAGNLPELQNK"
     gene            1116..1997
                     /locus_tag="PKW60_10880"
     CDS             1116..1997
                     /locus_tag="PKW60_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPJ99980.1"
                     /translation="MERRTFLTSCAAGAGAMAALSRTAIAAAKESKPFANLPLKVVAP
                     YSWFDGSPAERLAQAAAWGLPAVEWLGPDKSADELRDASEKTGVKWSCIGGVGAIAPG
                     QMVQPKEHDKLVEDFRERVAFGKSIGVTTFVGLTGNARDDISYDRHMTYVIRCLRRLA
                     PIAEENGVTLVMESLNTLVDHHGYFLSKTDQAMLLLEAVDSPNVKMLFDIYHQQIMEG
                     NVIRNLTENIDRIGHIHVGDNPGRKQPGTGELNYANIFKAIASTNYQGYVALECGYTT
                     ATGDEALAQVADCFNWT"
     gene            2223..3065
                     /locus_tag="PKW60_10885"
     CDS             2223..3065
                     /locus_tag="PKW60_10885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPJ99981.1"
                     /translation="MHDITVLTLIPNAGDRLARCLESVQWADDIFCVVDPRTSDGSDA
                     MARQYSSRVVVHEYVNAATQRNWALPQIETEWTLVLDADEWVSPELAARIQQIIRDPA
                     SCDGYHVKRMSYFMGRLMRHCGWGRDYNVRLFRTAKGRYLDRRVHSKVQVEGSMGRIH
                     EVMFHDTYRDFDEYFRTFQRFTAWGAEDLFEKGRRASWSDLTLRPALRFFKMYVINRG
                     FLDGYHGAVLSMLGAFSVFAKYAKLWRLGYTDAECRPPRDANALGPGGPDGEVDDAAP
                     ETRR"
     gene            3100..>4022
                     /locus_tag="PKW60_10890"
     CDS             3100..>4022
                     /locus_tag="PKW60_10890"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPJ99982.1"
                     /translation="MRYVMGCAVALGVCLMGLAAAQESALWLHPLCEKLDIPYNGPFE
                     QLSDGTLLVIDRNVLRKSVDGGVTWEAVSGEITPGLDINYGGHPGQFLQTRNGTIVVV
                     YLDMANYVFSWNDEKGEPNPECRLELWTIRSADGGKTWSDAQQLLPGYNADFMGFIQL
                     DSGRLVATVEHLDPELCRWVALSFVSDDEGQTWQRGNIIDLGGRGHHDGAVEPCVAVL
                     SDGRLLMFIRTGLGQFWYAFSEDNGKYWRIIRPSGIDASSAPGWVERLASGRLVFVWN
                     RAKPEGAEKPTSFWHGQAHEVPAPSHREELS"
BASE COUNT          825 a         1239 c         1262 g          696 t
ORIGIN      
        1 acaaggtcga ccgcacccgc aagcagatcg aaagcaacga cgcgttcgcc aagaaacgcg
       61 acatcaaaga cgaccattgt ttcaccgggc tcgatgccta caagggcatc ctgaagaccg
      121 acatcgatat cctcctggaa ggctcgcttc cctattgccg tccgatgcac ctggaagccg
      181 ctgtcgaggc aaagaagcac attttcacgg agaaacccgt tgcggtagac cccgtcggca
      241 tccaccaggt catccgcgcc gcgaaacgcg ccgacgagct gggcctctcc ttcgtggcgg
      301 gcacacagcg ccggcaccag aggccctacc gggaaaccat cgccaagatt cacgaaggcg
      361 ccatcggcga catcgtgtcc ctgcgcgcgt actggtgcgg cggccttccg tttgcccatg
      421 aacgcaagcc cgaatggacc agcgacctcg aatactgcat ccgcaactgg tacgcccact
      481 gctgggtctg cggcgacaac atcgtcgagc agcacgtcca caatctcgac gtctgcaact
      541 gggtcatgga aggctttggc atcgagcacc cgatttcggt gtttgcctcg ggcggccggc
      601 agttcaagcc caatgagccc aaatacggtg atctctggga ccacttctcg tgcgatttcg
      661 aataccccaa tgacgtacat atgaccagct tcagccgtca ctgggacggg ctcgacgacg
      721 gaatcttcga ggccgcgtat ggcaccaaag gcataagcaa ctgccgcgac atgggcgagt
      781 atggccccga tccgtacgtt caggagcaca tcgatctcgt gaacagcatc accggtgaag
      841 gccgccatta caacgaaggc gtgcaagtgg cgtacagcac catgaccgcg atcatgggcc
      901 gcatctctgc ctacaacgga cgccgggtga cctgggaaga tgtgatggaa ggcaagctga
      961 aagattactc gatcgtgccc gagctggatt gggaccgggc ctatccgaac gagccggttc
     1021 cggtgcccgg aaaggtcggt gcgggtaatc tccccgaatt gcagaacaaa tagagcgcaa
     1081 ctgcggaaac cgtggaggaa ccaagcacca tggatatgga acgtagaacg tttcttactt
     1141 cgtgcgcggc gggtgcgggc gccatggccg ccctgagccg tacggccatc gccgcggcta
     1201 aggaatccaa gccgtttgcc aacctgccct tgaaggttgt agcgccgtac agctggtttg
     1261 acggaagccc ggccgaacgc ctggcgcagg ctgccgcgtg ggggctgccc gccgtcgaat
     1321 ggctcggtcc cgacaagagc gccgacgagt tgcgcgacgc gtccgaaaaa acgggcgtca
     1381 agtggagctg catcggcggc gtgggcgcca tcgcccccgg ccagatggtt cagcccaagg
     1441 agcacgacaa actcgtggag gacttccgcg aacgggtggc gttcggcaag agcattggcg
     1501 tcaccacctt cgtgggtctt acggggaacg cccgcgacga catctcctac gaccggcaca
     1561 tgacctacgt gatccggtgc ctgcgccgtc tcgcccccat tgccgaggag aacggcgtga
     1621 cgctcgtgat ggaatcgctg aacaccctcg tcgaccacca cggctatttc ctgtcgaaaa
     1681 ccgatcaggc catgctgctc ctcgaggccg tggacagtcc caacgtgaag atgctcttcg
     1741 acatctacca ccagcagatc atggaaggca atgtcatccg gaatctcacg gagaacatcg
     1801 atcgcatcgg ccacattcac gtgggcgaca atcccggccg gaaacagccc ggcaccggcg
     1861 aattgaacta cgccaacatt ttcaaggcca tcgccagcac gaactaccag ggctatgtgg
     1921 ccctggagtg cggttacact accgccaccg gcgatgaagc tctggcgcag gtcgccgact
     1981 gcttcaactg gacctgagaa gaccccgaac ggactctatg cgggcggcgc gcctgaacaa
     2041 ggccccgccc gcctttctta ttgggcaggg agtcagtaaa atggcgccaa cgtgaaggcg
     2101 ggggaacaac gcggcccgcg ggggcgcccg atgggagacc acggccgtca ctcgcgcaag
     2161 cgattgcagc aggcgcgcct tcaagcgatc cccggggcga tttccgggcg aggagctggt
     2221 acgtgcacga tatcactgtc ctgacactca tccccaatgc gggcgaccgc ctggcgcgat
     2281 gtctggaaag tgtgcaatgg gccgatgaca ttttctgcgt cgtggacccc aggaccagcg
     2341 acgggtccga cgcgatggcc cggcagtatt ccagccgtgt ggtcgttcat gaatacgtca
     2401 atgcggccac gcagcgcaat tgggccctgc cgcagatcga aaccgaatgg acgctggtgc
     2461 tggacgccga cgaatgggtg tcgcccgagc ttgccgcgcg catacagcaa attatccggg
     2521 atcctgcgag ttgcgacgga taccacgtga aacgcatgtc atatttcatg ggacgcctga
     2581 tgcggcattg tggctggggg cgggactaca atgtgcggct gttccggaca gcgaaggggc
     2641 gctacctcga tcgccgcgta cactcgaagg tgcaggtcga gggctccatg ggccgcatcc
     2701 acgaggtgat gttccacgac acctatcgcg attttgacga atatttccgc actttccagc
     2761 ggttcacggc ctggggtgcg gaagatttgt tcgaaaaagg ccgacgcgcc tcatggagcg
     2821 acctcacgct gcggccagcc ctgcgcttct tcaagatgta tgtcatcaac cggggttttc
     2881 tcgatggata ccacggcgcg gtgttgagta tgctcggcgc attctcggtt tttgcaaaat
     2941 acgcgaagct gtggcgcctg ggctataccg atgccgaatg ccggccgccg cgcgatgcaa
     3001 acgcgctcgg tcccggcgga ccggacgggg aagtggatga cgccgcgcct gagacacgcc
     3061 gctgagccgg tcctggcgcc ttcacgggag gaaagtctga tgcggtatgt gatggggtgt
     3121 gcggtggcgc tcggcgtgtg cctgatgggc ctggccgcgg cacaggaatc ggccctgtgg
     3181 ctgcatccgc tctgcgagaa actggacatt ccttataacg gcccgtttga acagctcagc
     3241 gacggaacgc tcctggtcat cgatcggaac gtgctgcgca agagcgtcga cggtggagta
     3301 acctgggagg ccgtcagcgg cgagatcacc ccggggctcg atatcaacta cggcgggcat
     3361 ccgggccagt tccttcagac ccgcaacggg acgattgtcg tggtctacct cgacatggcc
     3421 aactacgtct tcagctggaa cgacgaaaaa ggcgagccca accccgaatg ccgcctggaa
     3481 ctctggacga tccgaagcgc ggatggcggc aagacctgga gcgatgccca gcagcttctg
     3541 ccgggctaca acgcggactt catgggcttc attcaactcg attcagggcg cctcgtcgcc
     3601 acggtggaac acctggatcc ggaactctgc cgctgggtgg ccctgtcgtt tgtctcggat
     3661 gatgaggggc aaacctggca gcgcggcaat atcatcgacc tgggcgggcg aggccaccat
     3721 gacggcgccg tggagccgtg cgtggccgtc ctgagcgacg ggcggctgct catgttcatc
     3781 cgaacggggc tgggccagtt ctggtatgcg ttttcagagg acaacggcaa gtactggcgg
     3841 atcattcgcc caagcggcat cgatgccagc agcgcgccgg gctgggtcga gcggcttgcg
     3901 agcgggcgcc tggtcttcgt gtggaaccgc gccaaacccg agggcgctga aaaacccacc
     3961 tcgttctggc atggccaggc ccatgaagtg cccgcgccca gccaccgcga agaactctcg
     4021 at
//
LOCUS       DAOPBQ010000347         4017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18284_length_4017_cov_3.512367, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000347 DAOPBQ010000000
VERSION     DAOPBQ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18284_length_4017_cov_3.512367"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKW60_10895"
     CDS             complement(<1..436)
                     /locus_tag="PKW60_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family peptidase"
                     /protein_id="HPJ99983.1"
                     /translation="MQRKTDIIAYAGCVVGVLLVALAPAAAQETSGLDRLLRAYPGHL
                     IATEKPNVLLWKDGTEMIFDDGIPKADFDDLLNRASLKDQMSQPYPRSWPATAPALNA
                     DPGRVRNEAFFRKLYGGSADEVRANLVPVDWLGGTSVQFTKVG"
     gene            592..1479
                     /locus_tag="PKW60_10900"
     CDS             592..1479
                     /locus_tag="PKW60_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99984.1"
                     /translation="MMVTRRRFVQLSAAAGMAVMAGRDSRAEGRERVRDRLWLWSHHP
                     GVYDGMFGLPRNSRITPVDAARYLGTPNIMFIRASGQPPYPFEDYAAAFKDVRRLQWS
                     ITGSAGVTSHEEREYALQLAASMPNITGLYMDDFFNFAREGQEASMTVEEVEAIRERM
                     TVNGRRLDLGIVVYTADQFDERMLPYLQLCDAISLWSWSASELDGIEDHFARLREIAP
                     GKHLQMGCYMWDYGPQQPMPLERVERQCALGLRWLREKRIHDMIFCGAHLCDQGLDAV
                     EAARSWIARVGHQPLHDPA"
     gene            1816..2820
                     /locus_tag="PKW60_10905"
     CDS             1816..2820
                     /locus_tag="PKW60_10905"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPJ99985.1"
                     /translation="MNLRALVWGVLVLCCASVPGLAEPAPEAVNDPFMGEYEGVYHWV
                     GHPDIPATGLIVAEGPGLYRMVAQCQTETGQNNYVELHGQLVGPRVFFHGYSGSGQWD
                     AEAAQDTLNVKGAYKTSFELKKIERRSPTEGQTPPEGAVVLLPFEPGKAPDLSAWTNG
                     NWKAYDDGSMGVQPGTGPNTTRAKIGDCKMHLEFYLPLMAGAFGQGRSNSGVYMQNRY
                     EIQLLDSFGVQPQTAGDCGGLYDISTPVVNATLPPERWQTYDIEFHAARLNEDGSQKE
                     PARLTVRHNGVVIQGDVALAGPTPGGTDGPGAAEDILHLQDHGNRVRFRNIWYVPLND
                     "
     gene            complement(3794..>4017)
                     /gene="ssrA"
                     /locus_tag="PKW60_10910"
     tmRNA           complement(3794..>4017)
                     /gene="ssrA"
                     /locus_tag="PKW60_10910"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          772 a         1170 c         1265 g          810 t
ORIGIN      
        1 cgccaacctt tgtgaactgc acgctcgtgc cgcccaacca gtccaccggg accaggttcg
       61 cgcggacttc atccgcggaa ccgccgtata acttccggaa aaaggcttcg ttgcggacac
      121 gtcccggatc ggcattgagc gccggcgcgg tggcaggcca tgaccggggg tacggctgag
      181 acatctggtc tttcaggctg gcgcggttga gcagatcgtc aaaatcggct ttcggtatgc
      241 cgtcgtcaaa gatcatttcc gtgccatctt tccacaagag gacgttcggc ttttccgtcg
      301 cgatgagatg cccggggtag gcgcggagca gacggtccag cccgctggtt tcctgcgctg
      361 cggcgggcgc cagcgccacg agcagcacgc ctaccacgca acccgcatac gcgataatgt
      421 cagtttttcg ttgcattgcc gcagtataca cgagcacaca tgcggcggcg agcgcctgcg
      481 cggccgacgc gcgtgccacg ccctcatgat tctctgttgg gtcgatttct tcgttcccgt
      541 ttttcgtggt agcatgggag gcggtcctgc gggcggcagc agaaaggggg cgtgatggta
      601 actcgacggc ggtttgtgca actgagcgcg gcggcgggaa tggccgtgat ggcgggccgg
      661 gattcacggg ccgaagggcg tgaacgggtt cgcgaccgcc tgtggctctg gtcgcatcat
      721 cccggcgtgt atgacggcat gtttgggttg ccgcgaaact cgcggataac tcccgtggac
      781 gccgcgcgct atctgggcac gcccaacatc atgtttattc gcgccagcgg ccagcctccg
      841 tatcccttcg aggactacgc agccgcgttc aaggatgtcc ggcggctcca gtggtcgatc
      901 acgggcagcg ccggcgtcac gtcgcatgag gaacgcgagt acgcgctcca actcgccgca
      961 tccatgccca atatcacggg tctgtacatg gacgatttct tcaacttcgc ccgcgaaggc
     1021 caggaagcgt ccatgacggt ggaagaggtt gaggccattc gtgagcggat gaccgtcaac
     1081 gggcggcggc ttgacctggg tatcgtcgtg tacactgcgg accagtttga cgagcggatg
     1141 ttgccctatc tgcagttgtg cgatgcgatc tcgctctggt cgtggtctgc gagcgaactg
     1201 gacgggattg aagaccattt tgcacgattg cgagagattg cgcccggcaa acatctgcag
     1261 atggggtgtt acatgtggga ttacgggccg caacagccta tgccgctcga gcgcgtcgaa
     1321 cgccaatgcg cgctcggcct tcggtggctt cgagagaaac gcatccacga catgatcttc
     1381 tgcggcgccc atctctgcga ccagggcctc gatgctgtcg aggcggcccg gtcgtggatc
     1441 gcacgcgttg gccaccagcc gctgcacgac ccggcctgag cccggtagac acggacgctg
     1501 ctgcacatcc ggatagccct gcggctgcgt taaagaaggg cagacacagg ggtctgcccc
     1561 tacgcgctgc gaaaacttgg atggacgaag aaagtcccaa ccttgccact cagctctatg
     1621 agtgcgccga ttcccactta ttcatgcatc cggaatgtag gggcggcccc ctgtggccgc
     1681 ccatcttatc ggtacgcgtt gactcaaata cagggctgcc tccgccggca attggcatcc
     1741 gcgccctcca ggggataaga tggtttcttc aacgtaccca tgaaggcatg caagaactca
     1801 gagaggattt gcacgatgaa tctacgagcg ttggtgtggg gagtgcttgt gttgtgttgc
     1861 gcgtcggtcc cgggtctggc cgagccggcg cccgaggcgg tcaacgatcc gtttatggga
     1921 gagtatgagg gcgtctatca ttgggtgggc catcccgata tcccggcgac aggtctgatc
     1981 gtcgcggagg gtccgggcct gtatcgcatg gtggcgcaat gccagaccga aaccggtcag
     2041 aacaactacg tggagcttca cggccagctc gtggggccgc gcgtgttctt ccacggctac
     2101 agcggttcgg gccaatggga cgccgaggcc gcgcaggata cgctcaacgt aaaaggcgcc
     2161 tacaagacct ccttcgagct caaaaagatc gaacggcgtt ccccgaccga ggggcagacg
     2221 cctccggaag gcgcggtggt cctgcttccc tttgaaccgg gaaaggcgcc ggatctcagt
     2281 gcctggacca acggcaactg gaaagcctac gacgacggga gcatgggcgt gcagcccgga
     2341 accggcccaa acaccacccg ggccaagatc ggcgattgca aaatgcacct ggaattctat
     2401 ctgcctttga tggccggcgc gttcggccag gggcgaagca acagcggcgt ctacatgcag
     2461 aaccgttacg agattcagtt gcttgattct ttcggcgtgc agcctcagac cgctggcgat
     2521 tgcggcggac tgtacgacat ctcaacgcct gtggtgaacg ccacgctccc gcccgagcgc
     2581 tggcaaacct atgacatcga gttccacgcc gcacgcctta acgaagacgg ttcgcagaag
     2641 gagcccgccc ggttgacggt tcgccacaat ggcgttgtca ttcaagggga cgtcgcgctt
     2701 gcagggccca cgcccggagg caccgatggc cccggcgcag cagaagacat cctgcatctg
     2761 caggaccatg gcaaccgggt gcggttccgc aacatctggt acgtgccgct gaatgattga
     2821 tagactggcg ggagaggaca atcctggcgg acgagtgtgc gctggacggg tgagcgctgc
     2881 tttcgttcag gcaacccgtt gatgccgcgc gattctgcgt ccggcttgcg gttgtgccac
     2941 aggttccagc ggacgagtgc ctgcacattg cgggcactgc ccgagcagga gttcgggaat
     3001 ctcgggccag gtgtatttgc cgagccgctg cgcgcaatgg aagcaaagag cggagaatcc
     3061 gggcgtcaat ttttcgcagt cttccatggc ttgtctcctc acggacgaga ccacccgctg
     3121 tggcgtcgaa gtggttcaag cgtcgttctg cagacaaaaa cgggaggggc gaatgagcgc
     3181 ccctcctcga aggttctcga ctctggtgtg cggctggtcc gcaggcggag tatgcctgcg
     3241 gacgccgtca cactatttct tgcatcccga tttcttggcc gcaggctttt tcgcggcggt
     3301 cttcttcttt gctacggctg gcttcttcgc tgccactttc ttctttgctg ctggcatgtt
     3361 ctttctcctt ctcaggttct cacaacgaca accctacacc gcagagtgta gcacatacta
     3421 ataaaaagta caatatattg tattgcgggg ttttcaagga aaccgcgaga atttcgaccc
     3481 ccaatggggg aattgaggtc tgaacaggcg cttcagggct tctttcctta ggaaaaggtc
     3541 agcttctatg gtttttcgac ttcattccgt tttaagcgtt tcccgggggc tccaggggga
     3601 aaagtatccc tcaccggtgg taatgagaag ccgacggccg acagagggaa tttcgagcaa
     3661 aagaacgaga aattgctcca aatcctgcgg gaatggagtt cagacgtgaa aaaaaccgaa
     3721 aaatcccggc ggagcggtga ttttgcgcgg ttgtacggtt atttcgtcca aaaacgctct
     3781 agagcgtaac aaaaggtgga ggcggcggga atcgaacccg cgtccgaagg tgcgtccacg
     3841 tgcgcgtcta cgtgcgtagc ctgtcgtttg aggattcgcc gcccggaacc gcctcaggca
     3901 ggctttcccg ggtcgctagg cgaaatcggt actcgccttc cggacgtttc gccggcgtcc
     3961 ggagacgagg cccgctgtcg tcgccttagc cacctagcag gcggtcagtg gtaagac
//
LOCUS       DAOPBQ010000348         4001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18373_length_4001_cov_4.190066, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000348 DAOPBQ010000000
VERSION     DAOPBQ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18373_length_4001_cov_4.190066"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKW60_10915"
     CDS             <1..225
                     /locus_tag="PKW60_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein"
                     /protein_id="HPJ99986.1"
                     /translation="MMAKEVFAEIVKLKDLGVTVFLVEQNAAATLSIADRAYVMETGE
                     IILEGDASELLHNAAVKRAYLGKGYKEIWE"
     gene            222..1406
                     /locus_tag="PKW60_10920"
     CDS             222..1406
                     /locus_tag="PKW60_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99987.1"
                     /translation="MKVLHRRYETMPRDELEQLQLERLQALLARLKRNVRRYRELLGE
                     TRIESLADIGQLPCTTPETLAEAFPYGMFALPLREVVRLHPTLGFRGTPLVTGYTRTD
                     LAQWGRLVARQLVASGVTVNDVVQVCLGSGVYRGASGYVMGAETIEASVIAEEPFHID
                     SQLAMLQNYRPTMLITTPDNARELMETLERRRMDPPSLHLRTILLTRPVPQETRQALE
                     AGLLVAVRCNFGVDEILNPGFCVECDAGHFHVNEDQFLVEAQDDELVVTTLVREAVPL
                     LRYRTRISATLRRERCPCGRTGAILEPGPRLDGRLRVRESSLYECQIAEVLAQTRAAG
                     HRFHVEVAERHVIVAIEMSSALFSDVMWMLTDLQNELQNEFKMRLGLDADIRLITPRG
                     KD"
     gene            1548..2828
                     /locus_tag="PKW60_10925"
     CDS             1548..2828
                     /locus_tag="PKW60_10925"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPJ99988.1"
                     /translation="MQTESPAFQALCDAIAAIPLIDTHEHIPPETTLAGRTWSLFDFF
                     EHYVCSDLVSAGMSHEALETLRNPNNGLSLEERWKLLAPYWPYAKTTSYGRAMTRYMS
                     DLFGIDDINEDTYAELSRRINEARSQPGWYRTVLKDKANIDCSLVITWPGEPVAVDQA
                     LFRAVPILDHYAIPGTRDDLALLEKEAGRSIQTLDQLLDAQEARLDAFARQRIAAVKI
                     FLAYRRTLRFDRFSKAEAGRAFDRIWLSQSQDLTFGDLKPLQDFMTRTLIGRAAERGL
                     PIQIHTGLQEGNGNYLENSNPLHLTNVIMEYSDARFDLFHAGYPFTGELAVLAKYFTN
                     VYADLCWVQAISPHAFKRTLHEWIDTIPANKIFAGGGDSNYVEGAYGHCTLARRLVAE
                     VLAERVEQGAMDQAGALWYAGRILRENALEFFPL"
     gene            complement(2850..>4001)
                     /locus_tag="PKW60_10930"
     CDS             complement(2850..>4001)
                     /locus_tag="PKW60_10930"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPJ99989.1"
                     /translation="ISVVVMTAYATVQTAVTAMKEGALDYLAKPFDKDELLVVVGKAL
                     EQAELRRENRQLRELISDTTALGNLVGQSPAMQKVFDTVRRATRVSSTVLIQGESGTG
                     KEMVARHIHFNGPRKARPFIVVNCAAIPDTLVESELFGHEKGAFTGAEASRQGKFEVA
                     HGGTIFLDEIGDMQLESQAKLLRVLQDGVVERVGSSQSRHVDVRVLAATNRDLKAVVA
                     DGQFREDLYFRLDVLPIRIPPLRERIEDLALLIDHFRQKLAKRFGRPAPAVSAAAMDA
                     MKRYRWPGNVRELENTLEQVFILCDKDSIGVDDLPEKFKTREPQTGDVQLPSGGLILE
                     EVEQDLIRQALARSGGRIKEAAELLGLSYKTLQYRLKKHDIDKHEAGSS"
BASE COUNT          765 a         1358 c         1153 g          725 t
ORIGIN      
        1 atgatggcca aagaggtctt tgcggagatc gtaaaactta aagatctcgg cgtcaccgtc
       61 ttcctggtcg agcagaacgc cgccgccacg ctgtccatcg ccgatcgcgc ctatgtcatg
      121 gagacgggcg aaatcatcct ggaaggcgat gcgagcgaac tgctgcacaa cgcggcggtc
      181 aagcgggcct atctcggaaa aggatacaag gagatctggg aatgaaggtg ctgcaccggc
      241 gctacgagac gatgccccgc gacgaactcg agcagctcca gctcgaacgc ctgcaggcgc
      301 tcctggcgcg actcaaacgc aacgtccgcc ggtatcgcga gcttctgggt gaaacccgca
      361 tagaatcgct cgcagacatc ggccaattgc cctgcaccac gcccgaaacc ctggccgagg
      421 cctttcccta cggcatgttc gccctgcccc tgcgcgaagt ggtccgcctc catcccaccc
      481 tcggattccg gggcacgccg ctggtcacgg gctacacgcg cacggacctg gcccaatggg
      541 gccggctggt ggcgcgccag ctggtcgcca gcggcgtcac ggtgaatgat gtggtacagg
      601 tctgcctggg cagcggggta taccgcggcg catcgggata tgtcatgggc gccgaaacca
      661 tcgaagcctc cgttattgcg gaagaaccgt ttcacatcga ttcgcaactg gccatgctgc
      721 agaattaccg gcccaccatg ctcatcacca cgccggacaa cgcccgcgaa ctcatggaga
      781 ccctggaacg ccgccggatg gacccgccgt cgctccattt gcgcaccatt ctcctgaccc
      841 gtcctgtccc gcaggaaacg cgccaggccc tggaggccgg cctgctcgtg gccgtacggt
      901 gcaatttcgg cgtagacgag atcctgaacc cggggttctg cgtcgaatgc gacgccgggc
      961 atttccatgt gaacgaagac cagtttctcg tcgaggcgca ggacgacgaa ctggtggtga
     1021 ccaccctcgt gcgtgaagcc gtgccgctct tgcggtaccg cactcgtatc tccgccacgc
     1081 tccgccgcga acgatgccca tgcggccgta ccggcgccat tcttgaaccc ggcccccggc
     1141 tcgacgggcg gttacgcgtg cgcgaatcgt cgttgtacga atgccagatc gccgaggtgc
     1201 tggcacagac ccgcgccgcc gggcaccggt tccatgtcga agttgccgag cgccacgtca
     1261 ttgtcgcgat cgagatgagc agcgccctct tttctgacgt catgtggatg cttaccgatc
     1321 tgcagaacga acttcagaac gagttcaaga tgcgcctggg cctcgatgcc gacatccgcc
     1381 tgatcacgcc ccggggcaaa gactgagcgc agcagcccgt ggaaaatatc ggacccgcgt
     1441 caggtgttcc ccctccaata tggctacgtt tgcccccacg tcttcgtgta ccatagaaag
     1501 atcaaatggc acggccgcag ccaaaccgga tgaaggaacg catttccatg cagacagaaa
     1561 gccccgcctt tcaggcactg tgtgacgcca tcgccgccat tcctttgatc gatacccacg
     1621 aacacatccc gccggaaacc acgctcgccg gacgcacctg gagcctcttt gatttcttcg
     1681 agcactacgt ctgctccgac ctcgtatccg cgggcatgtc gcacgaggcc ctcgaaacac
     1741 tgcgcaatcc gaacaacggc ctgtcgctgg aggaacgctg gaagctcctc gccccctatt
     1801 ggccctacgc aaaaaccacc agctacggcc gtgccatgac ccgctacatg agcgaccttt
     1861 tcggcatcga cgacattaat gaagacacgt atgccgagct gtcacgccgc atcaacgaag
     1921 cccgcagcca gccgggctgg taccgcaccg tgctcaaaga caaagccaat atcgactgca
     1981 gcctcgtgat cacctggccc ggcgagcccg tggcagtcga tcaggctctc ttccgggccg
     2041 tgcccattct cgaccactac gcgatacccg gcacgcgcga cgacctggca ctacttgaga
     2101 aggaggcggg ccgaagcatc cagaccctgg accagctgct cgacgcccag gaagccaggc
     2161 tcgacgcgtt cgcccggcag cgcatcgccg cggtcaagat cttcctcgcc taccgccgga
     2221 cgctccgctt cgaccgcttc tccaaggccg aggccgggcg ggcgtttgac cgcatctggc
     2281 tcagccagtc ccaggatctc acgttcggcg accttaaacc gctgcaggac ttcatgaccc
     2341 gcaccctgat cggccgcgcc gccgaacgcg gtctgcccat tcagatccac accgggttgc
     2401 aggagggcaa cggcaactac ctcgagaaca gcaacccact gcacctcacc aacgtgatca
     2461 tggaatacag cgacgcccgg tttgacctgt ttcatgccgg ttaccccttc acgggggaac
     2521 tggccgtgct tgccaagtat ttcaccaacg tctacgccga tctctgctgg gttcaggcca
     2581 tttcgcccca cgccttcaaa cgcaccctcc acgaatggat cgacaccatc cccgccaaca
     2641 agatcttcgc gggagggggc gattccaact acgtcgaggg cgcctacggc cattgcacgc
     2701 tcgcgcggcg gcttgtcgcc gaggtgctgg cggaacgggt ggagcaggga gcgatggatc
     2761 aggccggggc gctgtggtat gccggccgca tcctgcgcga aaacgccctg gagtttttcc
     2821 cgctgtaagc ttcccgcgct ggagacggct cacgagctgc cggcttcgtg cttgtcgata
     2881 tcgtgttttt tgaggcggta ttgcagggtt ttgtacgaaa ggcccagaag ctcggccgct
     2941 tccttaatcc gcccgccgga acgggccagt gcctgccgga tcaggtcctg ctcaacttct
     3001 tcgaggatca acccgcccga cggcagctgc acatcgccag tctgaggttc gcgcgtcttg
     3061 aacttttcag gcagatcgtc aactccaatg gaatccttat cgcagaggat aaagacctgt
     3121 tcgagggtat tttcaagctc gcgaacgttg cccggccagc ggtaacgttt catcgcgtcc
     3181 atggccgcgg cgctgacggc gggggccgga cggccgaacc gcttcgccag tttctgccgg
     3241 aaatgatcga tcagcagggc caggtcttca atccgttcgc gcagcggcgg aatccggatc
     3301 ggcagcacat ccagccggaa atacaggtct tcccggaact gaccgtcggc caccacggcc
     3361 ttcaggtcgc gattcgtggc cgcgagcacg cgcacgtcca catggcgcga ctgcgacgaa
     3421 cccacccgct cgactacgcc gtcctggagc acccggagca gtttggcctg gctttcgagc
     3481 tgcatatcgc cgatttcatc gaggaaaatg gtgccgccgt gggcgacctc gaatttgccc
     3541 tggcggcttg cctccgcgcc cgtaaaggcg cccttttcgt gcccgaacag ctcgctttcc
     3601 accagcgtat cggggatcgc cgcgcaattc actacaataa atggccgggc cttgcgcggg
     3661 ccattgaaat gaatgtgccg ggcaaccatc tccttgcccg tgcccgattc gccctggatc
     3721 agcaccgtgc tcgaaacgcg cgtggcgcgg cggacggtat cgaagacctt ctgcatggcc
     3781 gggctctggc ccaccaggtt gcccagagcg gtggtgtcgc tgatcagctc gcgcaactgc
     3841 cggttctcgc ggcgaagttc ggcctgttcg agcgccttgc ccaccaccac gagcaattcg
     3901 tccttgtcga agggcttcgc cagataatcc agcgccccct ctttcatcgc cgtgaccgcc
     3961 gtctgcactg tggcgtaggc cgtcatcacc acgaccgaaa t
//
LOCUS       DAOPBQ010000349         3981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18485_length_3981_cov_4.257769, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000349 DAOPBQ010000000
VERSION     DAOPBQ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18485_length_3981_cov_4.257769"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PKW60_10935"
     CDS             complement(<1..1043)
                     /locus_tag="PKW60_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPJ99990.1"
                     /translation="MATLPETSTEEVAAEVPAPAASRIRRALSVLVRMLLVIAILGVS
                     GYISYHWLLNPPVAQRRPPQREAALVEVMPAEMQTVRATVRNMGTVVPARTVDLAARI
                     RGQISKVAPAFLPGGHFAQGEVLAEIEPNDYELAVEQQRANLVKAQSELKLEMGQQSI
                     AQLEYELLGESAPEEDEALMLRQPQLESKKAAVAIAQAGLNQARLDLERTVLVAPFNA
                     VIQERRVDLGAYVNPGTALATLVDSDEFWVEVTVPVAELRWLQIPGFNSTSGSQVRIF
                     HEDAWGAGVYREGEVQRILSDIESGSTLPRILVAVKDPLQLNNGGEAPSHPLLLGAKV
                     RVEIIGQEIPDVCP"
     gene            complement(1092..2756)
                     /locus_tag="PKW60_10940"
     CDS             complement(1092..2756)
                     /locus_tag="PKW60_10940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HPJ99991.1"
                     /translation="MSEPDACHTPPACAKRPGLAPITAIGLVIALCGISCARFTPPER
                     SPDELVVPDEFALYDTSGAVPERWWKVFESDELNHLIERALSDNLSLRQMYSRLIQAE
                     MVAIQAGAARVPSLDYSGDISTGRRRVDTGKHADQLTTLNPKISAWNRLNAAGSIPPA
                     DVLDALDQAGAALKAIDTLTAGQPSSHSTFTTRSYRFGLGSSFEVDLWGRVRSQHEAA
                     LSDVEATREDLYGAMLSLSALVAREWLFAVTQRQTIALVERQLELNKTTLGLIELRHR
                     KGMATALDVYQQRQIVAGTESLLPPLESALQTTLHELAVLLGQEPRAELGVTQDGLPK
                     AGPLPAPGLPADLLARRPDIRAAGLRLRSADWRVSVARADRLPALRLTGAASYGADDW
                     DLVFNNWAATLAAGVTGPIFDAGRRKAEIERTRAVVDERLAAYQERVLVAAREVETAL
                     YEEVKQGEYIAALERELDAARDAHNQALLRYRKGSNDYLPVVSALSQLQVVERRLVQA
                     ELARLDLRVQLCVALGGSWMAPEHEAIRAREQAAAPETAAPLPRNG"
     gene            complement(2760..3419)
                     /locus_tag="PKW60_10945"
     CDS             complement(2760..3419)
                     /locus_tag="PKW60_10945"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HPJ99992.1"
                     /translation="MRIRKDGEETRKRILSAAFQVFGEKGYRDATHAAICQRAGVNTA
                     AINYHFGSKRGLYEATWAYATARADALYPLDGGVSPDASGEERLAGLVRAMLQRRADE
                     EQLGHLQSIRMMELVNPTGLLDDAIADWHETSRRHFLAAIRDLLGPGATETDLDLCEM
                     SVMSQCIMAFKRKRAWTSGSDKVDVLASHIVGFCLAGMAAVRTEIRQRNGGATPKQAG
                     A"
     gene            3618..>3981
                     /locus_tag="PKW60_10950"
     CDS             3618..>3981
                     /locus_tag="PKW60_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPJ99993.1"
                     /translation="MNPQSTFARELEVAVAAAQVAGGILREAFNQPGGPKGSPGHADA
                     DRDAEVAIRDALAGAFPEDGYLGEELYDARRAPGREGRLWVVDPNDGTSAYMKGFRGA
                     ATSIALLVKGQPVLGVVYA"
BASE COUNT          685 a         1364 c         1180 g          752 t
ORIGIN      
        1 gggcacacgt ccgggatctc ctgtccgatg atctccaccc gcaccttcgc tcccagcagg
       61 agcggatgcg acggggcttc gccgccgttg ttcagttgaa gggggtcctt gactgcaacc
      121 aggatgcggg gcagcgtgct gcccgattcg atgtccgaaa ggatgcgttg cacctcgcct
      181 tcgcgataga cgcccgcgcc ccaggcgtct tcatggaaaa tacgcacctg cgagcccgat
      241 gtgctgttga aacccgggat ctgcagccag cgcagctcag ctaccggcac cgtcacttcc
      301 acccagaact catccgaatc caccagcgtc gcgagcgctg tgccgggatt cacataggcc
      361 cccagatcga ctctccgttc ctgaatcacc gcgttgaatg gcgccacgag cacggtgcgt
      421 tccaggtcga gccgcgcctg attcaagcct gcctgcgcaa tagccactgc ggcctttttc
      481 gattcgagct gcggctgccg cagcatcagc gcctcgtctt cctcgggagc gctctcaccc
      541 agcagttcgt attcgagctg ggctatcgac tgctggccca tttcgagttt gagttccgat
      601 tgcgccttga ccaggttggc ccgctgctgc tctaccgcca gttcgtagtc gttgggctcg
      661 atctcagcca ggacttcgcc ctgcgcgaaa tgcccgcccg gtagaaatgc cggggccacc
      721 tttgagatct ggccgcgaat ccgtgccgcc aagtcgaccg tacgcgcagg caccaccgtg
      781 cccatgttgc gtacggttgc gcgaaccgtc tgcatctccg caggcatcac ctccacgagg
      841 gccgcctccc gttgtggcgg gcgccgctgg gccacgggag ggttcagcag ccaatggtac
      901 gaaatgtatc cgctgacccc cagaattgcg atcaccagaa gcatgcgcac aagcacgctc
      961 aaagcgcgcc gtatccgcga ggccgccggc gccggtactt cggcggcaac ctcttcggtg
     1021 cttgtttcag gaagtgttgc catgtgctgg tctgctccaa tcctccaccg gataccacct
     1081 ccggcgtcgt tttatccgtt tcgtgggaga ggcgcggctg tctccggagc cgccgcctgc
     1141 tccctggcgc gaatcgcttc gtgctccggg gccatccacg aaccgcccag ggcgacgcac
     1201 agctggaccc gcaggtcgag ccgggccagt tcagcctgta ccagccgccg ctcgactacc
     1261 tgtagctgcg acagcgccga tacaacgggc aaataatcgt tcgagccctt gcggtaacgc
     1321 agcagcgcct ggttgtgcgc atcgcgcgct gcatccagct cccgttccag cgcggcgatg
     1381 tattcgccct gcttcacctc ttcatataac gcggtttcga cttctctcgc cgcgaccagc
     1441 acacgttcct ggtaggcggc cagccgctcg tccaccacgg cgcgcgtgcg ttcgatttca
     1501 gcttttcgcc ggcccgcgtc gaagatcggc ccggttacgc ctgcggccaa tgtcgcagcc
     1561 caattgttga acacgaggtc ccagtcgtct gcgccgtagc tggccgcgcc cgtcaggcgc
     1621 agcgcaggca ggcggtccgc ccgggccacg ctcacccgcc agtccgccga gcgcaggcga
     1681 agccctgccg cgcggatatc cggccggcgc gccaggaggt cggctggaag gcccggcgcg
     1741 ggcaagggac ccgccttggg aaggccgtcc tgcgtcacgc ccaactcggc gcgcggctcc
     1801 tggcccaaaa gcactgccag ctcgtgcagg gtggtctgca gcgccgactc cagcggcggc
     1861 agcaacgatt ccgttcccgc gacgatctgc cgttgctgat aaacgtcgag cgcggtcgcc
     1921 atgccttttc ggtgccgcag ctcgatcagg ccgagcgtgg tcttattcag ctccaactgt
     1981 ctttcgacca gcgcgatcgt ctggcgttga gtcacggcaa acagccattc tctcgccacc
     2041 agggccgata gactgagcat cgcaccgtag aggtcttcgc gcgtcgcttc gacatcgctc
     2101 agggcggcct cgtgctgcga ccgtacccgg ccccacaaat ccacttcaaa actgctgccc
     2161 aggccaaacc ggtacgagcg cgtggtgaac gtgctgtgag acgacggctg gcccgctgtc
     2221 agcgtgtcga ttgccttcaa cgcggcgccg gcctggtcca gcgcatccag gacatccgcc
     2281 ggtgggatgc tgcccgccgc attgagccgg ttccaggccg agatcttcgg attcagagtg
     2341 gtcagctgat ccgcatgctt tcccgtatcg acccggcgcc gtcccgtcga gatgtcgcct
     2401 gaataatcca gcgagggcac ccgcgccgca cccgcctgga tcgccaccat ttcggcctgg
     2461 atcaggcggg aatacatctg acgcagcgac agattgtccg acagcgcccg ctcgatcagg
     2521 tggttcaatt catccgactc aaagaccttc caccagcgct ccggaactgc gccggacgtg
     2581 tcgtagaggg caaattcgtc agggaccacc agctcatccg ggcttcgctc cggcggggta
     2641 aagcgcgcgc aggagatgcc gcaaagggcg atcacgagcc cgatggccgt gatcggtgca
     2701 agcccgggac gtttcgcgca ggcgggggga gtgtggcagg cgtcgggttc ggacatgaat
     2761 tatgctccgg cctgttttgg ggtggccccg ccgtttcgct gccggatctc cgtgcgcacc
     2821 gcggccatgc ccgccagaca aaatcccacg atgtgtgatg ccagcacgtc gaccttgtca
     2881 ctccctgaag tccaggcccg ttttcgctta aacgccataa tgcattggct cattacgctc
     2941 atttcacaca aatccaggtc ggtttcagtt gctcccggcc ccagcaggtc gcgtatggct
     3001 gcaagaaaat gccggcgcga cgtctcatgc cagtccgcga tcgcatcgtc caacaggccc
     3061 gtcgggttca ccaactccat catgcggatg ctctgcagat ggcccagctg ttcctcgtct
     3121 gcccggcgct gcagcatcgc gcgcaccagc cccgccagcc gctcctcccc tgaggcatcc
     3181 ggagacacac ccccgtccag cggatacaac gcatcggcgc gcgcggtcgc gtaggcccac
     3241 gtcgcttcgt ataatccgcg cttggagccg aaatggtaat tgatcgccgc cgtattgacg
     3301 cccgcgcgct ggcagatcgc cgcgtgcgtg gcgtcccggt accctttttc cccgaatacc
     3361 tggaacgccg cgctcaaaat gcgcttacgc gtctcttcgc catctttacg gattcgcacc
     3421 aagtcagcct gcatcttatc aatcgcttag ttcaagtacg aagttgaact tgtaccacga
     3481 tcccgccctt aagtcaagaa gagaggccta tctcgaatca gccgatcgct cttgcttcgc
     3541 gcgtcccgca tgctaagata ggcgcgcctg cagtcagtat gtcatgccgc gtaaaaaccg
     3601 ggggtatact ctatatcatg aacccgcaga gtacgttcgc acgtgagctt gaagtcgcag
     3661 ttgccgcggc gcaggttgcc ggaggcatcc ttcgtgaggc gtttaatcag ccgggcggtc
     3721 ccaaaggcag cccgggccac gccgacgcgg accgcgacgc cgaggtagcc atccgggatg
     3781 cactcgccgg cgcctttcca gaagacggtt atctcggtga ggagctgtat gacgcgcgcc
     3841 gggcccccgg ccgcgaaggc cgtctctggg tcgtcgaccc caatgatgga acgagcgctt
     3901 acatgaaggg atttcgtgga gcggccacgt ccatcgcgct tctcgtaaaa ggacaaccgg
     3961 tcctgggcgt cgtttacgca t
//
LOCUS       DAOPBQ010000350         3981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18486_length_3981_cov_4.035660, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000350 DAOPBQ010000000
VERSION     DAOPBQ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18486_length_3981_cov_4.035660"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1079)
                     /locus_tag="PKW60_10955"
     CDS             complement(171..1079)
                     /locus_tag="PKW60_10955"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPJ99994.1"
                     /translation="MKIDIHGHVFADPRLISPLAGTPFMSAEQQLEVMEQKGIDMTVI
                     LPLTSAEAPAEKQSVGEVLGICARYPGKFIPFVNVDPRLPRDPAKITVEDHYRVLAEC
                     RDLGFKGLGELTARMYWDEHPMQCLLGACEKIGFPVTFHTITPDVDSYGVMDELGLPR
                     FEKCLQTFPDLVFFGHSPGFWSEISGDATVADKNAYPSRPVAEGGVLKRLFREYPGVH
                     GDLSAGSGFNALQRDPEHAYAFLDEFQDRLLLGLDYCSPKNKMEHVEWLTAARDAGHI
                     SDTVYEKIMWRNANRILDLGLADEPD"
     gene            complement(1116..2219)
                     /locus_tag="PKW60_10960"
     CDS             complement(1116..2219)
                     /locus_tag="PKW60_10960"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPJ99995.1"
                     /translation="MKGILCAAMAAGLVVVPVMAGSAAYAQDYTEGWINLFDGETLFG
                     LQPLGDTTWVADNGTLTCTGGIGGWIATTGQWGDFELTMKVRVDAKAATAVVLRAPLE
                     GHFTENGSVVIPIQGLDSGDGWQDVAVTAQGTSVSVQVNGKDVAADAPRNAVGHIGIA
                     YQGAQVQASAIKLRPLAMQPIFNGTDLTGWNIIPGRKSVFTVEDGAIRIKDGNGQIET
                     DGLYQNFVLQLDIFSNGDHLNSGVFFRGPKGVFWKGYESQVRNEWSGDDRNAPVDFGT
                     GGLYGIQPARKVVSSDREWFYKTILCNGNHFAVWINGYAVSDFLDMRAPVDDSNAKAG
                     FVGAAGTIHLQGHDPTTDLSFKNIRLQEYPSGE"
     gene            2466..3959
                     /locus_tag="PKW60_10965"
     CDS             2466..3959
                     /locus_tag="PKW60_10965"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPJ99996.1"
                     /translation="MPKHLVTSAFLAMLLLTVPAASPRAAEVTFESLVREMTDLDALA
                     SFPDPAFDCAQASSYDRRSTDPAVLTDENWFANGDRGHHIRKETRNGADEWVMLDAEG
                     PGAIVRIWSANAPDAGIVRIYLDNAPEPIIEMPLQDMLDGAHAPFLAPIAGVRALGWN
                     SHLPIPYARHCKITASQPNFYYQINYRTYTKNVDVESFRPEQALAAQDLLENTRQILA
                     SPDTLPQTGPRAITNYNETIAPGARVEMRLEGEGAIQTITCRPTARDLERALRQCVLT
                     ITFDDQKTPSVEAPLGDFFGTAPGINPYKSLPSGVHDDGTLYSQWVMPYRKSATLRLT
                     NHGDAPVEVDGAVVSGPRPWTPRSLYFHTTWRAQFDIPTQPRQDWTFVAMKGKGRFVG
                     DMLHVTNPVKNWWGEGDEKIYVDGETFPSHFGTGSEDYFGYAWCSNELFVHAYHNQPR
                     CDGPNNYGQTCVSRFHILDTIPFTKDFKFDMEVWHSVNTRVRPPYRI"
BASE COUNT          754 a         1392 c         1068 g          767 t
ORIGIN      
        1 atccctggcg aaagcggcgg tttcccccca caggtaatcc acggaaaacc ctttgatctt
       61 cttctcgacg ttgatgatgg tcgcgatcgc cttcgagtgt gagttttcag ccccgataat
      121 gcccagaatc atggctttac tctcccctgt gcgcgcccgg cgcggcaccc tcaatccggc
      181 tcgtccgcca ggcccagatc caggatccgg ttggcgttcc gccacatgat cttctcatac
      241 acggtatcgc tgatatgccc cgcgtcgcgc gccgcggtca gccattccac atgctccatc
      301 ttgtttttcg gcgagcaata gtccagcccc agcagcagcc ggtcctgaaa ttcgtccaga
      361 aacgcgtacg catgctcggg atcgcgctgg agcgcattga atccgctacc ggccgataaa
      421 tcgccgtgca cgcccggata ttcgcgaaac aggcgcttca gcacgcctcc ttcggccacc
      481 ggccgcgacg ggtacgcgtt tttgtccgcc acggtcgcgt caccgctgat ctccgaccag
      541 aatccggggc tgtgcccgaa aaacaccaga tcgggaaacg tctgcaggca tttttcgaag
      601 cggggcagcc ccagttcgtc catgacgccg tagctgtcca cgtcgggtgt gatggtgtgg
      661 aacgttactg gaaagccgat tttttcgcag gcgcccagca ggcactgcat cggatgttca
      721 tcccagtaca tgcgcgcggt cagctcgccc agacctttga atcccaggtc gcggcactcg
      781 gccagcaccc ggtagtggtc ctcgaccgtg atctttgccg ggtcgcgcgg caggcgcggg
      841 tccacattga cgaaggggat gaatttcccc ggatacctgg cgcagatgcc cagcacctcg
      901 cccacgctct gcttctcggc cggggcttcc gccgatgtca ggggcagaat gaccgtcata
      961 tcgatgccct tctgctccat gacttccagc tgctgctccg cgctcatgaa cggggtcccg
     1021 gccaacgggg agatcagacg cggatccgca aaaacatggc cgtggatatc gattttcact
     1081 gtggattctc cggtgccgcg cttttctggc tacgcctact cgcccgacgg atattcctga
     1141 agccggatgt ttttgaacga caagtccgtt gtcgggtcgt gcccctgcag gtggatcgtc
     1201 cccgccgcgc cgacaaaacc cgccttggcg ttgctgtcgt cgacaggcgc acgcatgtcc
     1261 agaaaatcgc tcacggcgta cccgttgatc cacaccgcaa aatggtttcc gttgcacaga
     1321 atggtcttgt agaaccattc ccgatcgctc gaaaccacct tccgggccgg ctggatgccg
     1381 tacaggccgc cggtgccaaa gtccacggga gcgttgcggt cgtcgccgct ccactcattt
     1441 cgcacctgcg actcatatcc tttccagaag acgcccttcg ggccccggaa gaacacgccg
     1501 ctgttgaggt ggtctccatt cgagaagatg tcgagctgca gcacgaaatt ctggtagagc
     1561 ccgtccgtct cgatctggcc gttgccatcc ttgatgcgga tcgcaccgtc ttccaccgta
     1621 aacactgatt tgcggcccgg gatgatattc catccggtca gatcggtgcc gttgaaaatc
     1681 ggctgcatcg cgagcgggcg cagcttgatc gcgctcgcct ggacctgagc gccctgatac
     1741 gcaatcccga tatggcccac ggcgttccgc ggggcatcgg ccgcgacgtc tttgccgttc
     1801 acctgaacac tgaccgaggt cccctgggca gtcacggcca cgtcctgcca gccgtcaccg
     1861 gaatcgagcc cctggatcgg gatgaccacg ctcccgttct ctgtgaaatg gccttcaagc
     1921 ggcgctcgca gcaccaccgc cgttgccgct ttcgcgtcca cacggacctt catcgtcagt
     1981 tcaaaatcac cccactggcc cgtggtggcg atccagccgc cgatcccgcc cgtgcaggtc
     2041 agcgtgccgt tatccgccac ccaggtcgta tcacccaggg gctgcagccc gaaaagcgtc
     2101 tctccatcga acagattgat ccagccttcc gtataatcct gcgcgtacgc cgcgctgccc
     2161 gccataaccg gtacaacaac caatcccgcc gccatcgcgg cacacaaaat gcctttcatc
     2221 gatgctctcc tcccaggtta tctccagatg gcctcccgca accggcgcca tccttaccgt
     2281 gtttaaagcg aaaccacatc ctacaccttc cctggcgctc ccgccaactt cgcggttacg
     2341 cggcaggggc cgcaccgccg cgcccgccag gacgccggtg gctcttcccc ggctggattt
     2401 cctgcaccgc ggccggtatt cttgccccag cgctccctga accagacccg aaggaggcct
     2461 gatccatgcc aaaacatctc gtgacgtctg cgtttctcgc tatgctgctc ctcaccgttc
     2521 cggctgcatc tccacgcgcg gccgaagtga cgttcgagtc gctggtgcgc gagatgaccg
     2581 atctcgacgc actcgcctcg ttccctgacc cggcgtttga ctgcgcccag gcgtccagct
     2641 acgaccgccg gtccaccgat ccggccgtcc tcaccgacga aaactggttt gccaacggcg
     2701 atcgcggcca tcacatccgc aaggaaaccc gcaacggcgc cgacgaatgg gtcatgctcg
     2761 atgccgaggg ccccggcgcg atcgtgcgta tctggtcggc caacgcgccc gacgccggca
     2821 tcgtacgcat ctacctcgac aacgcgcctg aacccatcat cgaaatgccg ctgcaggaca
     2881 tgctcgacgg ggcgcacgct ccgtttctcg ctccgatcgc cggggtgcgt gcactgggct
     2941 ggaattcgca tctcccgatc ccctatgccc gccactgcaa aatcactgcc agccaaccga
     3001 atttttatta ccagatcaac taccgaacct acacaaagaa cgtcgacgtc gaatcgttcc
     3061 ggccggaaca ggccctggct gcgcaggatc tgctggaaaa tacccgccag attctcgcca
     3121 gccccgacac cctcccccag accgggcccc gcgcaatcac caactacaac gagaccattg
     3181 cccccggcgc ccgcgtcgaa atgcgcctcg aaggcgaggg cgccattcag accatcacct
     3241 gccggcccac cgcccgagac ttggaacgcg cgctgcgcca atgtgtgctc accataacct
     3301 tcgacgatca gaagaccccg tccgtcgaag cgccgctcgg cgacttcttt ggcacggccc
     3361 ccggcatcaa tccgtataaa tcgcttccca gcggcgttca tgacgacggc accctgtaca
     3421 gccagtgggt gatgccgtat agaaagtcgg ccacgctccg cctgaccaat cacggggacg
     3481 ccccggtgga ggtggacggc gccgttgtct ccggacctcg cccctggacg ccccggagcc
     3541 tctatttcca taccacctgg cgcgcgcaat tcgacatccc aacccagccg cgccaggatt
     3601 ggaccttcgt cgccatgaaa ggcaaggggc gtttcgtcgg cgatatgctc cacgtcacca
     3661 accccgtcaa gaactggtgg ggcgaaggcg acgagaaaat ctatgtcgac ggtgaaacgt
     3721 ttccaagcca tttcggcacc ggcagcgagg actatttcgg atacgcgtgg tgcagcaacg
     3781 agctgtttgt gcacgcctac cacaaccagc cccgttgcga cggccccaac aactacggac
     3841 agacctgcgt cagccgcttt cacatcctgg acaccatccc ctttacgaag gatttcaaat
     3901 tcgatatgga ggtctggcac tcggttaaca ccagggtcag acctccatat cgaatttgaa
     3961 atccttcgta aaggggatgg t
//
LOCUS       DAOPBQ010000351         3978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18506_length_3978_cov_3.828448, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000351 DAOPBQ010000000
VERSION     DAOPBQ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18506_length_3978_cov_3.828448"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PKW60_10970"
     CDS             <1..790
                     /locus_tag="PKW60_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPJ99997.1"
                     /translation="HSGAPWRRIFHRSPPVESPEPAISGDTKPAGADGEEDATEELSE
                     APDGASEVGIADELAALRSDVARMQAMLDLVLDEFVISLKNENNRLREELETLEHALE
                     TREAMERAPFPPLSLPDAAQARGTPAVAERPPAYDAVAMPAPGESIRYAVIKEWGRNA
                     EEAARLNNASTLKGMICAVPPQASDDQLAELGRWLRSEYDAYENINIDVFDDVDAARR
                     FAETNSMEGGRRVLNVSRHPATNRDVIVLIRPDGTTVIPRETAP"
     gene            complement(792..1313)
                     /gene="hpt"
                     /locus_tag="PKW60_10975"
     CDS             complement(792..1313)
                     /gene="hpt"
                     /locus_tag="PKW60_10975"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPJ99998.1"
                     /translation="MQRRNEPLISEQAIQARISELAAEISREYANRELVLAVVLKGAL
                     MFAADLSRKLTVATSIEFVRARSYQGTQSSRRVQVTIFPEESLAGKHVLIVEDILDTG
                     YTTHFLLDRFREAKPDSLELCTLLDKPARRNIPVAAKYVGFSIDDLFVVGYGLDYEER
                     FRELPAVYVLDDI"
     gene            complement(1300..2688)
                     /gene="tilS"
                     /locus_tag="PKW60_10980"
     CDS             complement(1300..2688)
                     /gene="tilS"
                     /locus_tag="PKW60_10980"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPJ99999.1"
                     /translation="MKQKAPKLLDRVRDTLHRHDMLDADTPCLAAVSGGPDSMCLVQV
                     FHELRIPFEIAHFDHGTRAGESAKDAAFVREAAARYGVPCHETRRDIPRETAQSRESF
                     EELARRARYAFLLETAAARKCQALATGHHADDQAETVLLRLLRGTSPGGLGAIPPVRQ
                     AGDVRIIRPLLDCARDEILAYLDARDLPYRIDQTNTDTRHPRNRVRHELLPLLARHYN
                     PRVRDALRRLAEVQRDEDDFLQGLATDLADACRTGEQTIDRQAFAMMHPALQRRVVAL
                     LGWEFGIECPADRIELIRRHLLEGAAGKACDLGQGILLRNGREVTEIVEEPVHSEEAA
                     VTLAFPGETVAFGQRYTVRELEKSPAEELAAYCTPARQVFDAETLGTALAVRFRRPGD
                     RFSPLGLGGTKKLKDYFIDLGLTERERARQPLLTAQGTIVWVIGRAIGQTAAVTPSTR
                     RYVEIRVENATP"
     gene            complement(2716..2792)
                     /locus_tag="PKW60_10985"
     tRNA            complement(2716..2792)
                     /locus_tag="PKW60_10985"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2756..2758),aa:Met,seq:cat)
     gene            3058..>3978
                     /locus_tag="PKW60_10990"
     CDS             3058..>3978
                     /locus_tag="PKW60_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00001.1"
                     /translation="MRYGLSESSRFLHHLTRRAFLRSSVLAGGAATLSAPAIAQQTGA
                     NERLNIAIIGSGGRGAGNLEAVSSENIVALCDVYEPALAAAAQKFPQARTFVDFRKLY
                     DHADEFDAVVVSTCEHTHAFATLPALQLGKHVFCEKPLTHNVWEARIIREAANQARVA
                     TQMGTQIHASDNYRRVVELVQSGAIGAVGEVHVWVGRAWGWHATEEEAQAAKDIVFVQ
                     ERPTVEDPIPEGLHWDLWLGPAPARPFSEVYFPGPKWYRRWDFANGTMSDLGSHWIDL
                     PFWALDLDFPRTIEASGPPPHPELAPASMQV"
BASE COUNT          705 a         1205 c         1338 g          730 t
ORIGIN      
        1 gcattccggg gcaccctggc gccgcatttt ccacaggtcg ccgccggtgg aaagccccga
       61 gccggcgatc tccggagata caaagcctgc tggcgcagac ggtgaggagg atgcaacaga
      121 ggagctgtcc gaagcgccgg acggcgcgtc tgaggtaggc attgcggatg agctggccgc
      181 attgcggtcc gatgtggcgc ggatgcaggc gatgctcgac ctggtgctcg acgaatttgt
      241 gatcagcctg aaaaacgaga acaaccgctt gcgtgaagag cttgaaacgc ttgagcatgc
      301 gctggaaacc cgggaggcga tggagcgcgc gccatttcct cccttgagcc tgcccgatgc
      361 ggcgcaggca cggggaaccc cggccgtggc ggagaggccg ccggcgtacg acgcggtcgc
      421 gatgccagcg cccggcgaaa gcatccgcta cgccgtgatc aaggagtggg ggcgcaatgc
      481 agaggaggct gcccgtctga acaatgcctc caccctgaaa ggcatgattt gcgctgtgcc
      541 gccccaggcc tcggacgacc agcttgccga actggggcgc tggctgcgca gcgagtacga
      601 tgcttacgaa aacatcaata tcgatgtgtt cgatgacgtg gatgccgcgc ggcggtttgc
      661 ggaaaccaac agcatggaag gcgggcggcg cgtgctgaac gtatctcggc atcctgccac
      721 caaccgcgac gtcatcgtgc tgatccgtcc ggacggcacg acggtgatcc cgcgggagac
      781 cgcgccgtag gtcatatgtc gtcgaggacg tagacggcgg gcagttcgcg gaaccgctct
      841 tcgtaatcca ggccgtatcc caccacgaac aggtcgtcga tggaaaatcc gacatatttg
      901 gccgccacgg ggatattgcg ccgggccggt ttgtccagga gggtgcacaa ttcgagcgaa
      961 tcgggcttcg cctcgcggaa ccggtcgagc agaaaatgcg tggtgtagcc ggtatccaga
     1021 atgtcttcta cgatcaggac atgctttccc gcgaggcttt cttcagggaa gatggtgacc
     1081 tgcacgcggc ggctcgactg tgttccctgg tagctgcggg cgcgcacgaa ctcgatgctg
     1141 gtggcgacgg tgagttttcg cgacaggtcg gctgcgaaca tcagcgcccc tttgagcact
     1201 acggccagca ccagttcgcg gttggcgtac tcgcgggaga tctcggcggc cagctcggaa
     1261 attcgggcct ggattgcctg ttctgatatg agcggctcgt tacggcgttg cattttcgac
     1321 ccggatctcc acataacggc gggtcgacgg cgtcacggcg gcggtttgcc ctatggcacg
     1381 cccgataacc cacacgatgg tcccctgagc ggtgaggaga ggttgccgtg cgcgctcccg
     1441 ctctgtcaag cccaggtcga tgaagtagtc tttcagcttt ttggtgcctc ccagtccgag
     1501 ggggctgaac cggtctccgg ggcggcggaa tcgcacggcc agggcggttc cgagtgtctc
     1561 cgcatcgaaa acctgacggg ccggcgtgca gtacgccgcg agttcctctg ctggtgactt
     1621 ttccaattcg cgcacggtat accgctgccc gaacgccacg gtctctccag gaaaggccag
     1681 tgttacggct gcttcctcgc tgtgcaccgg ttcttcaacg atttcggtga cctcgcgccc
     1741 gttgcgcagg agaatgccct ggcccaggtc gcaggccttg cctgccgctc cctcgagcag
     1801 gtggcggcga ataagctcga tgcgatcggc ggggcattcg atgccaaatt cccagcccag
     1861 cagggccact acgcggcgct ggagagctgg atgcatcatc gcaaaggcct gccggtcgat
     1921 ggtctgctcg ccggtacggc atgcatccgc aagatccgtg gccagtccct gcaggaagtc
     1981 atcttcatcg cgctggacct ccgccaggcg cctcagcgcg tcccggacgc gcgggttgta
     2041 gtggcgtgcc agaagcggca gcagctcgtg gcggacgcgg ttgcgggggt gacgtgtatc
     2101 cgtgttggtc tggtcgatgc ggtacggcag atcccgcgca tcgagatagg cgagtatctc
     2161 gtcccgggcg cagtcgagca gaggccggat gatgcggacg tccccggcct ggcgaaccgg
     2221 cgggatcgcc cccagcccgc ctggagatgt gccgcggaga agccgaagca gtacggtttc
     2281 ggcctgatca tcggcgtgat ggcccgttgc gagggcttga cacttccgcg cagcggccgt
     2341 ttccagcagg aaggcgtacc gggcccggcg cgccagctct tcaaacgatt cccgcgattg
     2401 tgcggtttcc cgggggatgt cgcggcgggt ttcgtggcag gggacgccgt agcgggccgc
     2461 agcctcgcgc acgaaggcgg catccttcgc actttcgccc gcgcgcgtcc cgtgatcgaa
     2521 atgggcgatc tcgaagggaa tgcgcagttc gtgaaacacc tgaacgaggc acatggaatc
     2581 gggcccgccg gacacggcgg ccagacacgg agtgtccgca tcgagcatgt catggcggtg
     2641 aagggtatcg cgaacgcgat cgagcagttt tggggccttt tgcttcacgg ccggctcccg
     2701 gcggcaagag ggaaatggtg gcggcgcacg gatttgaacc gcggaccttg ggattatgat
     2761 tcccctgctc taaccaactg agctacgccg ccacacgggc cgtaagtgta ccaaacattt
     2821 ggccggcatg tccacttttg ttcacacgtt tctgccgaag ggggcgcacg gcgtaacggc
     2881 cgtgcttcac ggcggccagg ctggccgttt gagggcacgc cgccggtctt ttttttgcat
     2941 tgccgctctc gcgcactgtg ggcatgctgc atctcttgaa cacctggttc agggacagta
     3001 gactggcata aatgtgagca actgcaggca catgtacgac cgtgaaaggg taccgccatg
     3061 agatacggcc tctcggaaag cagcaggttc cttcatcatc tgacccgccg ggccttttta
     3121 cggtcctcgg tgcttgcggg cggggccgcc acactgagtg cgccggccat tgcgcagcag
     3181 actggcgcca acgaacgtct gaatatcgcc atcatcggtt cggggggccg cggcgccggc
     3241 aacctggagg cggtgagttc agaaaatatc gtggcgcttt gcgacgtgta tgagccggca
     3301 cttgcggcgg ctgcgcagaa atttccacag gcgcggacat tcgtcgattt ccggaagctc
     3361 tacgatcacg ccgatgagtt cgacgcggtg gtcgtcagta cctgcgaaca cacccacgcc
     3421 ttcgcgacct tgcccgcgct gcagttaggc aagcatgtct tctgtgaaaa acccctcacc
     3481 cacaacgtgt gggaagcgcg catcatccgc gaagcggcga atcaggcccg agtggccacg
     3541 cagatgggca cgcagattca tgcctcggac aactaccggc gcgtggtcga gctggtgcag
     3601 tcgggggcga ttggcgcggt tggtgaagtg cacgtctggg tggggcgcgc ctggggctgg
     3661 cacgccacag aggaagaagc ccaggccgcg aaggatatcg tgtttgtgca ggaacggccc
     3721 acggtagagg atcccattcc cgaagggttg cattgggacc tctggctcgg gccggccccg
     3781 gcgcggccgt tcagcgaggt ctattttccg ggacccaaat ggtaccggcg gtgggatttt
     3841 gcgaacggca ccatgtccga cctgggctcg cactggatcg acctgccgtt ctgggcgtta
     3901 gacctcgatt ttccccgcac catcgaagcc agcgggccgc cgccgcaccc cgagctcgcg
     3961 cccgcgtcga tgcaggtg
//
LOCUS       DAOPBQ010000352         3974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18526_length_3974_cov_3.982904, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000352 DAOPBQ010000000
VERSION     DAOPBQ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18526_length_3974_cov_3.982904"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PKW60_10995"
     CDS             complement(<1..730)
                     /locus_tag="PKW60_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK00002.1"
                     /translation="MPKPVIFAILLSLVAGAAVGSAPLSPELAHLEALSGNPALLLKM
                     VRGFDRLQCELANWDMELAEELALSGDTELAEEKMEQARKRLALVEEAYTYVLERYPD
                     NGRALTYYGELIYDYQGDHTKALMLWKKAAALNPELGDVFNNLGIHYSHAGDLSRGLD
                     YFDKALKCEPNNPDYLYNLCQTYLTQFPAVAKRKGWSLKKVYSTAMKMSKKAAELGDD
                     YELVQDYAVNFFAAENFQVKADWRQ"
     gene            complement(801..1751)
                     /locus_tag="PKW60_11000"
     CDS             complement(801..1751)
                     /locus_tag="PKW60_11000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK00003.1"
                     /translation="MRIRSVQTLPGNFAEYIHDVAGGMTELYGEAAGAAYRCIAAKAV
                     EASMHAPGVATFCAEIDGSCAGMAMTFHRDNLGRLPFIHVLRPYWGTGVEGRLVREAV
                     SVLKADGASAIHCECIPFCDLDLRGVFKKLGFIVFPRQLMMASTELLSNIGTNVSRSA
                     PCGPLDLRVAAATIAEIYKRHPERVLHPELRDARSVERYMRDAMQGAFGQCYPEYVRV
                     VRSHDGIRGVIVGCEVAPGVGFVLQVAVRPDAQNQGLGRALIADLASSFLKRGMKHTA
                     LGVTLENPARNLYLRLGFEPRRDVDAFVWPPSLNCNPSCP"
     gene            complement(1799..2977)
                     /locus_tag="PKW60_11005"
     CDS             complement(1799..2977)
                     /locus_tag="PKW60_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPK00004.1"
                     /translation="MHRAPVLLVSLMLWTAFVWAQEPLPGLAVGEGGVLLKGGAPYRG
                     IGVNYFDAFLRRLRDPEAVHCREGFKVLSGKGIPFARFAACGFWPVDWQLYHEDKDAY
                     FAQLDDVVRAAEETGLGLIPSLFWFNACVPDMMGEPRSAWGDPESKTIAFMRQYVADV
                     VPRYVHSPAIWVWEMGNEYDLAADLPNAETQRPPVVPRLGTPSERSEKDDMTTEMIVT
                     ACREFALAVRQHDPHRAITTGNSLPRPAAHHMHTERTWARDTREQFQERLGLTSPDPI
                     NLVSTHVYPFDLDTRFGDGTTYPELLSLAIAACRDAGKALFVGEFGAGGDGDQAGSQG
                     PRHEHELILDALVTCGVPLAAIWVYDLEQQKDSFSITPDHDRAYLLDRIVEANKRMQP
                     "
     gene            3242..>3974
                     /locus_tag="PKW60_11010"
     CDS             3242..>3974
                     /locus_tag="PKW60_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HPK00005.1"
                     /translation="MPLVPMRQILDEAAKGGYGVGAFNVNNMEQIQAIMEAANETNSP
                     VIIQASRGALQYSRLIYLKKLMEAACEEYPHIPVSMHLDHGNSLETLKTAIDLGFTSV
                     MMDGSLGEDGKTPNTYEQNVAITRAAVEMAHPLGVTVEGELGVLGGIEDGHGAGLSDS
                     EVQDHLTDPNQAEDFVQKTGLDALAVAIGTSHGAYKSGRKDPKTGKVLPPTLAMDRIH
                     AIHEKMPHCHMVMHGSSSVPQELVDI"
BASE COUNT          819 a         1238 c         1151 g          766 t
ORIGIN      
        1 cctggcgcca gtcggctttc acctggaaat tttcggccgc aaagaaattg accgcatagt
       61 cttgcaccag ctcgtagtca tcgcccagtt cggcggcttt tttcgacatt ttcatggccg
      121 tgctgtagac ttttttgaga ctccacccct tccgcttggc cacggcggga aactgagtaa
      181 gataggtctg gcacaggttg taaagataat caggattatt gggctcgcat ttcagggcct
      241 tatcgaagta gtccagtccg cggctcaaat ctcccgcatg cgaatagtgg atccccagat
      301 tattgaacac atcgcccagt tcgggattca gtgcggcggc cttcttccac agcatgaggg
      361 ccttcgtgtg gtccccctgg taatcataaa tcagctcgcc ataatacgta agcgcccggc
      421 cattgtccgg ataccgttcg agcacatagg tataggcctc ttcgaccagg gccaggcgct
      481 tcctggcctg ttccatcttt tcctcggcga gctccgtgtc acctgaaagg gccagttcct
      541 cggccagttc catgtcccag ttcgccagct cgcactgaag gcggtcgaat ccgcgaacca
      601 tcttcagcag aagcgcagga ttaccggaca gcgcctccag atgggccagt tccggggaaa
      661 gcggtgcgct gccgacggca gcacctgcca ctaagctcaa aaggattgca aaaatcaccg
      721 gtttcggcat ggcagttgtc tctcaatgcg gttcgttcat agatgccgcg attggggcta
      781 ctataacatg ccaggcgttt tcacggacaa cttggattgc aattcagtga cggcggccag
      841 acaaaggcat ccacgtcgcg gcggggttcg aaacccaggc gcagatagag gttgcgcgcc
      901 gggttctcca gtgtaacgcc caaagccgta tgcttcatgc cgcgtttcag gaagctggag
      961 gccaggtcgg cgatcagggc tctgcccagc ccctggttct gcgcgtccgg ccgaacggcc
     1021 acctgaagga caaatcccac gcccggcgcc acttcgcagc ccacgatcac cccgcgaatc
     1081 ccatcgtgac tccgaacaac ccgcacatac tcgggatagc actggccaaa ggcgccttgc
     1141 atcgcgtcgc gcatgtatct ctcgactgaa cgcgcatccc ggagttcggg atgcaggacg
     1201 cgctccgggt ggcgtttata aatctccgca atcgtcgccg ccgcgacgcg caaatccaac
     1261 gggccgcagg gagccgaacg cgatacgttg gtcccgatat ttgagaggag ctcggtgctc
     1321 gccatcatca gctggcgcgg aaagacgatg aagcccagtt tcttgaaaac gccgcggagg
     1381 tccagatcgc aaaacggaat gcattcgcaa tggatcgcgg acgctccgtc cgccttcaac
     1441 acgctcacgg cctctcgcac caggcggcct tcgacgccgg ttccccagta agggcgcaac
     1501 acgtgaatga acggaagacg gcccagattg tctcgatgga atgtcatggc catccccgcg
     1561 cagctgccgt cgatttccgc gcagaaagtg gccaccccgg gcgcatgcat tgacgcctcc
     1621 acggccttcg ctgcaataca gcggtaagcc gcgccggcgg cttcgccgta caattccgtc
     1681 atccctcccg caacgtcgtg gatgtactcc gcaaagttgc cgggaagtgt ctgaaccgat
     1741 cgaatgcgca ccgcatccct ttcctttcac gtggaaaatg ttaagtggag accggccgtc
     1801 aaggctgcat ccgtttattg gcctcgacaa tgcgatcgag caggtaggcg cggtcatggt
     1861 cgggcgtgat gctgaaactg tctttctgct gttccaggtc gtagacccag atggctgcca
     1921 gggggacgcc gcaggtgacg agcgcatcga ggattagttc gtgttcgtgc ctgggcccct
     1981 gggagcctgc ctgatctccg tcgccgcccg cgccgaattc gcccacaaac agggccttgc
     2041 cagcatcccg gcaggcggct atggccagcg acagtagctc cgggtaggtt gttccatcgc
     2101 cgaaacgcgt atccagatcg aatgggtaca cgtgtgtcga gaccaggttg atcgggtcgg
     2161 gactggtcaa gccgaggcgc tcctgaaact gttcgcgtgt atcgcgcgcc cacgtgcgtt
     2221 ctgtgtgcat atggtgagcc gcgggccgtg gcaggctgtt gccggtggta attgcgcggt
     2281 gggggtcatg ctggcgcacc gccagcgcaa attcacggca ggccgtcacg atcatttcgg
     2341 tagtcatgtc gtctttttcg ctgcgttccg aaggcgtccc gagccggggg accaccgggg
     2401 gccgttgtgt ttccgcattg ggcagatccg ccgccaggtc gtactcattt cccatttccc
     2461 agacccagat ggccggtgaa tgaacatagc gcgggaccac gtcggccacg tactgcctca
     2521 taaatgcgat ggtcttgctt tccgggtcac cccaggcgct gcgcggttcg cccatcatgt
     2581 cgggcacgca ggcgttgaac cagaacaggc tggggatcag accgagcccc gtctcttccg
     2641 cagcgcgcac cacatcgtcc agctgggcaa aatacgcgtc cttgtcctca tggtacagct
     2701 gccagtcgac gggccagaac ccgcatgcgg caaagcgcgc gaacggtatc cccttcccgg
     2761 aaagcacctt gaacccttcg cggcaatgga cggcctcggg gtcgcgcaaa cggcgcaaaa
     2821 atgcatcaaa ataattgacc ccgatgccgc gatacggcgc gccgcccttg agcagcactc
     2881 cgccttcccc taccgccagt cccggaagag gctcctgggc ccatacgaaa gcggtccaca
     2941 gcatcaggct cacaagcaaa accggcgctc tgtgcattac attttctccg tatttccgat
     3001 ggttccggca cagaatacgt ccccgccgtt gttactacaa ctgagcggcg gccgcgccct
     3061 gaacgcctta cgcgcgaaat ttgctgattt gcggcatcaa acggcgaaat gctatagtat
     3121 tgacttccat gtgcgctgcc ttgccctgtc tggggaaccg tttgaacgcc cgtatacgcc
     3181 aggacaggtt tcaggctctg gatttcaagg taaccgtcag tctagatagg agaacagtac
     3241 catgccactc gttccaatgc gacagattct ggatgaggcc gcgaaaggcg gatacggtgt
     3301 gggcgccttc aacgtcaaca acatggagca gatccaggcc attatggagg cggccaacga
     3361 aaccaacagc ccggtcatta tccaggcgag ccgcggcgcc ctgcaataca gccgcctcat
     3421 ctacctcaag aagctcatgg aagcggcctg cgaggaatac ccgcacattc ccgtgtccat
     3481 gcacctggac cacggcaaca gcctcgagac tctgaagacc gccatcgacc tcggatttac
     3541 gtccgtgatg atggacggct cgctcgggga ggacggcaag accccgaaca cctatgaaca
     3601 gaacgtggca atcacccggg cggcggtcga aatggcccat ccactgggtg tgacggtcga
     3661 aggcgaactg ggcgtgctgg gcggcatcga agacggccat ggcgccggtt tgagcgacag
     3721 cgaggtgcag gaccacctga ctgatccgaa tcaggcggaa gacttcgtcc agaagacggg
     3781 tctcgacgcg ctggccgtgg ccattgggac cagccacggc gcctacaagt ccggccgcaa
     3841 agacccgaaa accgggaaag tgctgccgcc tacgctggcc atggatcgca tccatgccat
     3901 ccatgagaaa atgccccact gccacatggt catgcacggc tccagctcgg tgccgcagga
     3961 actggtcgac atca
//
LOCUS       DAOPBQ010000353         3972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18538_length_3972_cov_4.131733, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000353 DAOPBQ010000000
VERSION     DAOPBQ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18538_length_3972_cov_4.131733"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PKW60_11015"
     CDS             complement(<1..597)
                     /locus_tag="PKW60_11015"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPK00006.1"
                     /translation="MRFLRILAWILGSAAALFVVVVATCLLLNYRTPSPADLTQSVLL
                     ASPPAPAGGEITLKFVTFNIWDLYHEGTHRQERMAAIGRALQEMQPDLIGFQEAFIAR
                     DRRIILNALAECGLNHSVYCHSGLVGSGLLVASRYPIEETFFHRYTRGGKPHKVHHGD
                     WWAGKGVCVVRVKLPDAIGYLDFFDTHAHARYGTDEYKD"
     gene            860..1981
                     /locus_tag="PKW60_11020"
     CDS             860..1981
                     /locus_tag="PKW60_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK00007.1"
                     /translation="MTASAQSAPFPSVSVVVPTYREADNIPLLVERIARVREEHRLEL
                     ELLIMDDDSNDGTEEVVRGLQHPWIRLIVRRENRGLGPAVVDGMLLARNDVLVCMDAD
                     LSHPPEAIPELLEALSQDYDFAIGSRYVAGGSTDESWGLLRRLNSRVATLLARPFTSA
                     EDPMSGFFALRRATFAAARDLNPIGYKIGLELLVKARCEYIKEVPIHFTDRRLGESKL
                     TLAEQLRYLQHLRRLFIYRYGGWAHFSQFAVVGGLGTLVNLGILTVLDLLGVPLAVGV
                     AVAIIGAMVFNFILNRRFTFSYARQGSLLRQFAGFAGASSVGALANYAVTLALVRQSA
                     FLARFPQAAALAGIAAGMFINFGISRYLVFRKPRRPRPV"
     gene            2029..3330
                     /locus_tag="PKW60_11025"
     CDS             2029..3330
                     /locus_tag="PKW60_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00008.1"
                     /translation="MALKVSRRQFVQSGTLAAAGAVSALVSSRAAGANERIRLGFIGV
                     ANRGGQLLGCTTPFEDAEVVALCDVYRPTIEKWAESVPNPVTLYSDYRRMIEREQLDA
                     VVIATPDHWHALQTIDACEAGLDVYVEKPLSITIHEGRRMIEAVRRTNRVVQVGIQRR
                     SCPTLAQIRDFVQEDKIGPVVVGRCCRVSNMWPDGIGNPPDSDPPAGLDWDMWLGPRP
                     YRPFNAAIAPYNFRWHKEYSSQLANWGVHYFDAFRMVLDEDVPTSICAMGGRLLLDDA
                     RSIPDTLEAVYSFPSRRMVHFSQYETSSNALLRTGMVEFRGTIGTLYYQGANFEVVPE
                     FGGQFQDREKRMEPMQVKGEVNDPTALHIRNFLDCVKSRATPNCSIEEGHKSTTLALL
                     GNISFEIGERIEWDPVAERVTNHAKANELLHYEYREPWKLA"
     gene            3627..>3972
                     /locus_tag="PKW60_11030"
     CDS             3627..>3972
                     /locus_tag="PKW60_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00009.1"
                     /translation="MRVAKGANREFRATRVFACTVMGLFSLTAAGQNLLVNPSAERGD
                     LSGWTILAAPAGGWRAGALPLAQMSSVPGRDPHSPDGCSVFFTDGSISSSNLNWCRRC
                     QIVDLMASGYTEE"
BASE COUNT          738 a         1152 c         1281 g          801 t
ORIGIN      
        1 atccttgtat tcgtcggtgc cataacgcgc gtgggcatgg gtatcgaaaa aatcgaggta
       61 tccgatggca tcagggagtt tgacccgtac gacgcacacg ccttttcccg cccaccagtc
      121 gccgtggtgc actttgtggg gttttccgcc ccgggtgtag cggtgaaaga aggtttcctc
      181 gatgggatag cggctcgcaa caaggaggcc gctacccacc agcccgctgt ggcaatagac
      241 ggaatgattc aggccgcatt cggccagcgc gttcagaata atcctccgat cgcgtgcaat
      301 gaaagcctcc tggaacccaa tgaggtccgg ctgcatttcc tggagcgccc tgccgatggc
      361 ggccatgcgt tcctggcggt gtgtgccttc atggtacaga tcccagatat taaacgtgac
      421 gaacttgagg gtgatctcgc cgccagccgg cgcaggtggc gaggccagca gcacgctttg
      481 ggtcaaatcc gccggcgagg gggtgcggta attcagcagc aggcaggtgg ccacgaccac
      541 cacaaacagg gcagcggcgc tgcccagaat ccacgccaga atccgcagga atcgcatcac
      601 ggcgccatcc tttcggcctt ttcggtcacg gacatttttc taaacgatgg gaggcactgt
      661 aatgaacggg cggcgaaaat tacaacggag ccgaactctc gagaaatagc aattttctcg
      721 tcccgatgct tggtcatgct atttcagcgt ttgacgttat cgcggacaac cacgtacact
      781 cccatgacat gcgcacgtgc aaaaattggc ggaaggggag cgtgtggctc gcggtcgggc
      841 ctgctggaga accgtctgta tgaccgcgag cgcacagtcc gcaccgtttc cctcagtatc
      901 ggtcgtggtc ccgacgtacc gggaggcgga caatatcccc ctgctggtcg agcgcattgc
      961 ccgtgtgcgg gaagagcatc gtcttgagct cgagctcctg atcatggacg atgacagcaa
     1021 tgacgggacc gaagaggtgg tgcgcgggct gcaacacccc tggattcgcc ttatagttcg
     1081 cagggaaaac cgggggctgg ggccggcggt cgtcgacggc atgctcctgg ctcgaaatga
     1141 tgtgctcgtc tgtatggacg ccgatctgag ccacccgccg gaggccatcc cggagctgct
     1201 tgaggcactg tcgcaggact acgatttcgc catcggttcg cggtatgtgg ccggggggag
     1261 cactgacgag agctggggcc tgctccgccg tctgaacagc cgggtggcca cactgctggc
     1321 gcggcccttc acgagtgcgg aagaccctat gagcggtttt tttgcgcttc ggcgcgcaac
     1381 gttcgcggcc gcccgggacc tcaatcccat cggctacaag atcgggctgg aactgcttgt
     1441 gaaggcgcgg tgcgagtaca tcaaagaggt gcccattcac ttcaccgatc ggcggctggg
     1501 cgagagcaag cttacgttgg ccgagcagtt gcgctatctt caacatctgc gccgcctgtt
     1561 catttatcgt tacggcggtt gggcccattt ttcgcagttt gcggtggttg gagggctggg
     1621 caccctggtc aacctgggga ttttgaccgt acttgatctg ctgggcgtgc cactggccgt
     1681 cggcgtggcg gtggcgatta tcggcgccat ggtgttcaat tttatcctga accgccggtt
     1741 caccttttcg tatgcgcgcc aggggtcact tctgcggcag ttcgcgggat ttgcgggggc
     1801 gagcagcgtg ggcgcgctgg caaattacgc ggtgactctc gcgctggtac gccagtcggc
     1861 cttcctggcg cgatttccgc aggcggccgc gctggccggc attgccgcgg gcatgtttat
     1921 taacttcggc atcagccgtt acctggtgtt tcgcaaaccc cgccgtccaa ggccggtgta
     1981 gccgcgcgcg gcgcgaacac cccatcatgg aacaggagga tttctgaaat ggccctgaaa
     2041 gtcagtcgca gacagtttgt gcaatcgggg actctggcgg ccgcgggcgc cgtctccgcg
     2101 ctggtttcgt cccgcgcggc cggcgccaat gagcgtatcc gcctggggtt tatcggagtg
     2161 gcgaaccggg gcggccagtt gcttggatgc accacaccgt tcgaagacgc tgaagtggtg
     2221 gcgttgtgcg atgtgtaccg cccaactatt gaaaaatggg cagagagcgt gcccaatccg
     2281 gtgacgctgt acagcgacta ccgccggatg atcgaacggg agcagctgga tgcggtcgtg
     2341 atcgccacgc cggatcactg gcatgccctt cagaccatcg atgcgtgtga agcgggcctt
     2401 gacgtctacg tggaaaagcc gctgtcgatc accattcatg aaggccgccg catgatcgag
     2461 gcggtgcgcc gcaccaaccg cgtggtccag gttggcattc aacggcgttc gtgtcctacg
     2521 ctcgcccaga tccgcgattt cgtgcaggag gacaagatcg gcccggtcgt ggttgggcgc
     2581 tgctgccgag tgagcaacat gtggccggat ggtatcggca acccccccga cagtgatccg
     2641 cccgccgggc tcgactggga catgtggctg gggccgcggc cgtaccgccc gtttaatgcg
     2701 gcgatcgcgc cctacaattt ccggtggcac aaggagtact cgtcgcaact ggcgaactgg
     2761 ggcgttcact atttcgacgc cttccgcatg gtcctcgatg aagacgtgcc gacctcgatc
     2821 tgcgccatgg gcggacgcct tcttctggac gatgcccgca gcatcccgga caccctggag
     2881 gctgtgtact cgttcccgtc gcgccgcatg gtgcatttca gccagtatga aaccagcagc
     2941 aatgcgctgc tgcgcacggg catggtcgaa tttcgcggca ccatcggcac gctctactac
     3001 cagggggcga atttcgaagt tgtgcctgag tttggcggcc agtttcagga tcgtgagaaa
     3061 cgcatggaac ccatgcaggt caagggcgaa gtgaacgacc caacggctct gcatatccgt
     3121 aatttcctgg attgcgtcaa atcgcgcgcg acgcccaact gcagcatcga ggaagggcac
     3181 aaatcgacga ccctggcgct gctgggcaat atctcgtttg agatcggcga gcgcattgag
     3241 tgggatcccg tcgcggaacg cgtcaccaat cacgcaaagg cgaatgaatt gctacactac
     3301 gaataccgcg aaccgtggaa actggcgtaa cgtccagtat tttcctgatg tcgttcgcct
     3361 cgaacccggc atctgccgcg gaatgccgcc gtgaccgctg cgcgcggcat tccgcgtcta
     3421 cacgcggcac ataatccttg ttccaaggta tactgtgggt gcggggctgg tgtaatcgct
     3481 gtgccggcgt gatggccgat gccaggggcg tgatgtggaa tcgctcacgc gctgtcaacg
     3541 cagagcccgg aatacgtgcg catggtgcgc ggaacaacga tcctgaggca gttgcggaaa
     3601 cgggccgtaa tggccagggg gtttttatga gagtggccaa gggtgcaaac agggaattcc
     3661 gtgcaaccag ggtgtttgcg tgcacggtga tgggcctttt tagcctgacc gcggcgggac
     3721 agaacctcct ggtgaacccg agcgcggaga ggggcgatct cagtgggtgg accattctgg
     3781 ctgctccggc cggcgggtgg agagctggag ccctgcctct tgcccagatg agcagcgttc
     3841 cgggacgcga tccgcacagc ccggacggat gcagcgtgtt tttcaccgac ggctccattt
     3901 cctcgagcaa tcttaactgg tgccgccgct gccagatcgt cgatttgatg gcctcgggtt
     3961 atacggagga ac
//
LOCUS       DAOPBQ010000354         3972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18542_length_3972_cov_3.461322, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000354 DAOPBQ010000000
VERSION     DAOPBQ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18542_length_3972_cov_3.461322"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PKW60_11035"
     CDS             complement(<1..348)
                     /locus_tag="PKW60_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00010.1"
                     /translation="MPDTEMQIFKVIMQGGVLMIPIGLCSIVALAIIIERFYSLRRAS
                     IDTREFMDTMRQVLRQNRIQDAVEICDEVDAPIARIMRAGILKYNRTKEDIREAIEDA
                     GHLEIPRLERYLSA"
     gene            complement(392..1267)
                     /gene="argB"
                     /locus_tag="PKW60_11040"
     CDS             complement(392..1267)
                     /gene="argB"
                     /locus_tag="PKW60_11040"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPK00011.1"
                     /translation="MQSKMSEFIQKASILIEALPYIREFDGKTVVIKYGGAAMENATL
                     RKSVAEDITLMKYVGMNPVVVHGGGPEINRRLKQLNVEASFHNGLRVTDEETIRVVEM
                     VLAGTVNKEIVALLNTAGGNSVGICGKDGNLLHAQRIELPDGADIGFVGRITQVNSGI
                     IDTLCRTGFIPVVAPLATDREGNTWNINADTAAGEIAASLQAEKLVFMTDTPGLLRDA
                     KDPETLIHRIERDEITKLKDLGVIAGGMIPKVDSCLLALDQGVRKTHIIDGRVPHALL
                     LEIFTTQGLGTLVTR"
     gene            complement(1292..2497)
                     /gene="argJ"
                     /locus_tag="PKW60_11045"
     CDS             complement(1292..2497)
                     /gene="argJ"
                     /locus_tag="PKW60_11045"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPK00012.1"
                     /translation="MSALEGGITAPKGFRASGVAAGIRRNSSKNDCALVVSDRAASVA
                     GMFTTNVMKAPPVEWNVGVCAKGAARAVFVNSGNANAATGERGHRDARAMADAVAAHL
                     GAPADEVCICSTGVIGVPLPMDRVLDGITRCAASLSVEGHGDAARAIMTTDTVPKELA
                     CEVQLSAGTVRIGAMAKGSGMICPNMATMIAVLSTDATVEAPVLSEMLRDAVERSFNC
                     ISVDNDMSTSDTVLCLANGAAGLPAIAQGSPDYAAFHAALSGICQSLAKMLVKDGEGA
                     TKLVEIAVSGAGSDSEAKTLARSIGLSQLCKTAFFGGDPNWGRIVCAAGYAGVPFDPA
                     DVALWLDEVHVVSGGMPLAYREEDAAAVMRRPEFQIRLEVGNGPGSAVFWCSDLSYDY
                     VKINADYRT"
     gene            complement(2494..3504)
                     /gene="argC"
                     /locus_tag="PKW60_11050"
     CDS             complement(2494..3504)
                     /gene="argC"
                     /locus_tag="PKW60_11050"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPK00013.1"
                     /translation="MIRAGVIGAVGYAGRELIRLLGIHPEAELVAAIELEGGKKLGEV
                     MPALAKTTAVTLDTFDAERLADQCDVVFMALHGGTSMDLIPPLRKAGVKVIDMGPDFR
                     LKNTADFKRYYGMEHRAPELLDEAVYGLAPYYREQLAKTSLVAVPGCYVMSVLFPLRP
                     LVEGISQDFPVIVDTISGISGAGRSLHEVFHFPEMNENVRAYKLGIHQHTPEIEQELG
                     GSVMVQFSPHVGPYTRGILSTMTFRPKDGLDVAKCYERYADEPFLRVLGEGKLAEIRF
                     VRGSNFCDFGWVMDKRTGNLIVVSAIDNLFGGTAGMAVQCMNIMFGLDETKGLNFGGM
                     AP"
BASE COUNT          797 a         1368 c         1015 g          792 t
ORIGIN      
        1 cgcggagagg taacgttcca gcctggggat ttccagatgc cccgcgtctt cgatcgcctc
       61 gcggatgtct tctttcgtcc ggttatattt cagaatgccg gcgcgcatga tccgtgcaat
      121 gggcgcatcg acttcatcgc agatctccac cgcatcctga atccggttct ggcgaagcac
      181 ctgccgcatc gtatccatga attcccgcgt atcgatcgac gcccggcgca ggctgtaaaa
      241 acgttctatg atgatggcca gcgccacgat ggaacagagt ccaatcggga tcatcagcac
      301 gccgccctgc atgatcacct tgaagatctg catctcggta tccggcacgg aacatccctc
      361 cttctcagat gcaagaaacc gccgtaaaat tctatcgggt caccagagtc cccagcccct
      421 gcgtcgtgaa gatctcgagc aggagggcat gcggcacgcg accgtcaatg atgtgcgttt
      481 tccgtacgcc ctgatccagc gccaggagac acgaatccac cttggggatc atgccgccgg
      541 caatcacccc caaatccttc aatttggtaa tttcatcccg ctcgatccgg tgaatcagcg
      601 tctcgggatc tttcgcatcg cgcagcagac ccggggtgtc cgtcatgaac accagttttt
      661 ccgcctgcag cgatgcggca atctctccgg ctgccgtatc cgcgttgatg ttccacgtat
      721 taccctcacg gtccgtggcc aatggcgcca cgacgggaat aaatcccgtc cggcacaacg
      781 tgtcgatgat cccgctgttc acctgggtta tccggcccac aaacccgatg tcggcgccgt
      841 caggaagttc aatccgctgg gcatgcagca agttcccgtc cttgccgcaa atccccaccg
      901 aattgccgcc ggccgtattc aacagcgcca cgatctcttt gttcaccgtg cccgcgagca
      961 ccatctccac cacgcggatg gtctcttcat cggtaacccg cagcccgttg tgaaacgagg
     1021 cctcgacgtt aagttgtttg agacggcgat tgatttcagg gccgccgccg tgaaccacga
     1081 ccgggttcat gcccacgtat ttcatcaggg tgatgtcttc cgcgacgctc tttcgaaggg
     1141 tcgcgttctc catcgcagcg ccgccgtact tgatcaccac ggtcttgcca tcgaactcgc
     1201 ggatatacgg caacgcctcg atcaggatcg aggccttctg aataaactcg ctcattttgc
     1261 tttgcatgtc gttccccaga aaacccgccc cctaggtacg gtaatcggca ttgattttga
     1321 cgtaatcgta gctgaggtcg gagcaccaga ataccgcaga acccggccca ttcccaactt
     1381 cgagccgaat ctgaaattcc gggcgcctca ttaccgcggc cgcatcctct tcacgatacg
     1441 ccagcggcat gcctcccgac accacgtgca cctcgtcaag ccacagcgcc acatccgccg
     1501 gatcaaacgg cactcccgcg tagcccgcgg cgcatacgat ccgcccccag ttgggatctc
     1561 cgccgaaaaa cgccgtcttg cagagctgcg acagcccaat cgaacgggcc agggtcttcg
     1621 cttcggaatc gctgcctgcg cccgaaaccg cgatctccac cagtttcgtc gcgccctcgc
     1681 cgtctttcac cagcattttc gccagcgact ggcatatccc ggacagcgcc gcatggaacg
     1741 ccgcgtaatc cgggctcccc tgggcgatcg cgggcaatcc cgccgctccg tttgccaggc
     1801 acagcaccgt gtcgctggtg ctcatgtcat tgtccacgga gatgcaattg aagctgcgct
     1861 ccacggcatc acgcagcatc tccgaaagca ccggcgcttc caccgtggca tcggtagaaa
     1921 gcacggcgat catcgtcgcc atgttcgggc aaatcattcc cgaacccttg gccatcgctc
     1981 cgatgcgcac cgtgcccgcg ctcaactgca cctcacaggc caattctttg ggcaccgtgt
     2041 ccgtggtcat gatcgcgcgc gccgcgtcgc catgcccctc tacggaaagc gaggctgcac
     2101 agcgggtgat accgtccagg acgcgatcca tcggcagcgg caccccaatc acgccggtgc
     2161 tgcaaatgca cacttcgtcc gcgggcgcgc ccaaatgtgc ggcaaccgca tccgccatcg
     2221 cgcgcgcatc ccggtgtccc cgttcgccag tcgcggcgtt ggcattgccg ctgttgacga
     2281 acacggcccg cgccgcgccc ttcgcgcaca cgcccacatt ccattcgacc ggcggcgctt
     2341 tcatgacatt cgtcgtgaac atcccggcga ccgacgcagc cctgtcactg accaccagcg
     2401 cacagtcgtt tttggacgag tttcgcctga tgcctgccgc cacaccggac gcacggaagc
     2461 ccttcggcgc agtaatgccg ccttcgagtg cgctcatggc gccatccctc cgaaattcag
     2521 gcccttcgtt tcatccagac cgaacatgat gttcatgcac tgcaccgcca tgcctgcagt
     2581 gccgccgaac agattgtcta tcgcgctgac cacgatcagg ttgccggtcc gcttgtccat
     2641 cacccatccg aaatcgcaga aatttgaacc gcgcacaaag cggatctcgg cgagttttcc
     2701 ttcgcccaat acccgcagga acggctcatc cgcgtaccgt tcgtaacatt tcgccacatc
     2761 gaggccgtct ttcgggcgaa acgtcatcgt gctgaggatg ccccgcgtat acggcccgac
     2821 atgcggcgag aactgcacca tcacgctgcc gccgagttcc tgctcgatct ccggcgtatg
     2881 ctgatgtatg ccaagcttat acgcacggac gttctcattc atttcgggaa aatggaacac
     2941 ctcgtgcagc gaacgtcccg caccggatat acccgagatc gtatcaacaa tcacagggaa
     3001 atcctgcgaa ataccctcga ccagaggacg caacgggaac agcacgctca tcacataaca
     3061 gcccggaacc gccacaagcg acgtctttgc gagctgctcg cggtaatagg gcgccaaacc
     3121 gtacaccgcc tcgtccagca attccggcgc gcggtgctcc atcccgtaat atcttttgaa
     3181 atcggccgtg tttttgagcc gaaaatccgg ccccatgtcg atcaccttta cgcccgcctt
     3241 ccgcaatgga gggatcaggt ccatggaggt cccgccgtgc aacgccataa acaccacatc
     3301 gcactggtcc gcaaggcgct ccgcgtcaaa cgtgtccagc gtcactgccg tcgtctttgc
     3361 cagcgcgggc atcacctcgc cgagtttttt gccgccttcc agctcaatcg ccgccaccag
     3421 ctccgcctca gggtggatcc ccagaagacg aatcagttca cgcccggcat acccgaccgc
     3481 accaattaca cctgctcgaa tcatctcaat ctcctcgttg caaaaaagta gccaattata
     3541 gccacgcgcc gtatccggtt caatttactc tttcgcacat tatgcgcaga attgcacgga
     3601 tcacggtggc agaacacctc caatgcaggc ttaagccggc ggaaatttgc tcgcctggca
     3661 tcgctgtgcg tttatgcccc ggcggctagc gtaaatgcca aggcgatatc gccacggtca
     3721 aatcataaat ggtgcgaatc gcgctctttt gttcttgaag tggccgagcg cggcagcgcc
     3781 gcaactgagc cacggggact gtgatccccc ggagtccacc cccaaatcgg ctgacgccga
     3841 ttcacccccg ccagcggggg acaagtgcgc cgcatccgga ttgcagaggc gacccatggg
     3901 tcgcctatct catcggtacg cgttgaccta aatacagggc aggcgccgcc gataccggct
     3961 cttgtgtccc cc
//
LOCUS       DAOPBQ010000355         3968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18562_length_3968_cov_4.406849, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000355 DAOPBQ010000000
VERSION     DAOPBQ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18562_length_3968_cov_4.406849"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="PKW60_11055"
     CDS             complement(<1..1193)
                     /locus_tag="PKW60_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HPK00014.1"
                     /translation="MRLDANVRVGKLVDQYPFLLDTLTSVSDKFKVLRNPLMRKTVGQ
                     FATLAKAAQLGEVPLAGLLESLANAIREKTGETVEIDAAEAAPGSPGAELDPERVAKL
                     REIIKGLHEGKDAGAVKEEFARLLQDVGPSEIAAMEQQLVQEGLPEEEITRLCDVHVA
                     VFRESLDRTSRVEAPSGHPVDTFRRENAAITEVIALLRKLLSEPDTADPERLSSEFAR
                     LAEIEKHYIRKENELFPQMEQYGVTAPPKVMWSIHDDIRAQLKEVRGALDRNDAATAQ
                     AQAPALLKAIEDMVYKEENILFPMCLELLSQDDWARVYHGEKEVGYTLIEPGPAWQPA
                     AAPAAQARPSGLNALPLDTGLLTLEQVNLLLTHLPVDLSCVDENGEVRYYSESPARIF
                     PRSSGV"
     gene            complement(1301..2284)
                     /locus_tag="PKW60_11060"
     CDS             complement(1301..2284)
                     /locus_tag="PKW60_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK00015.1"
                     /translation="MRQPLLELRDLRVHFPVYSGLIRRKLAGTVRAVDGVSLDVAPGE
                     TVGLVGESGCGKSTLARAVVRLAPCTGGTVYFEGQEISALRESAFRALRPRLQLIFQD
                     PYASLNPRMTVFDALAEPLHVHGLARGQELLRRVSNLMERVGLASRFMRKYPHEFSGG
                     QRQRIAIARALALEPRLIIADEPVSALDVSVQAQILNLIARLREQMGLTLLFIAHDLS
                     VVHYLSHRIAVMYLGRIVELGPASRVFHQPCHPYTRALISAIPVPDPGKEKTRQRVPL
                     HDEPASPLNPPPGCPFHPRCPWAEPRCAEGVPPLESRGERHQAACFRRDLP"
     gene            complement(2281..3255)
                     /locus_tag="PKW60_11065"
     CDS             complement(2281..3255)
                     /locus_tag="PKW60_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK00016.1"
                     /translation="MPLLDVHGLTTYFHTREGVVRAVDGLSFHIEPGETIGIVGESGS
                     GKTVASLSLMGLIPTPPGRIEKGTALFDGVDLLACGEQTLRTIRGQAISMIFQDPMTA
                     LNPYMRIGSQIIEPLRVHQKISQHDARERAAEMLRRVGIPDGERSLSAWPHEFSGGMR
                     QRVMIAMALVTRPQLLIADEPTTALDVTIQAQILELLAQLQEELGMAVLLITHDLGVV
                     ARMCRRTYVMYAGTVMESAATEDLFARPRHPYTEALLQSLPSMHVKGEPLHAIPGLPP
                     DLIAPITGCPFAPRCSQATERCSQGTISLQEIAPGHATACFRVQDGEL"
     gene            complement(3257..>3968)
                     /locus_tag="PKW60_11070"
     CDS             complement(3257..>3968)
                     /locus_tag="PKW60_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011468508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPK00017.1"
                     /translation="LLLGTPLAWWLARTASWARVPVRAVVALPLVLPPSVLGFYLLVT
                     LGPSGPGGQLTQALGLGLLPFTFAGLVVGSLLYSLPFVIFVVILTVYFGRNIVLLFMA
                     IGAVEWLTMARIVRAQVLSLRQREFIEATTVLGLPRYRVLLWHLIPNTLGPIIVYATL
                     TVPRVMLMEAFLSYLGLGVQAPMSSWGLLINEGVQTMENRPWMVIFPSLALSLTLFSL
                     NFLGDGLRDALDVRASKD"
BASE COUNT          765 a         1327 c         1211 g          665 t
ORIGIN      
        1 acccccgagc tgcgcgggaa aatgcgcgcg gggctttccg agtaataccg gacctcgccg
       61 ttttcatcca cgcacgagag atccacgggg aggtgtgtga gcagcaagtt gacctgttcc
      121 agcgtgagca ggcccgtgtc cagcggcagg gcattcaatc cggaggggcg cgcctgcgcc
      181 gccggggcag cagcaggctg ccaggccggc ccgggttcga tcagcgtgta gccgacctcc
      241 ttttccccgt ggtagacgcg tgcccagtca tcctggctga ggagttcgag gcacatcgga
      301 aagagaatgt tctcttcttt gtagaccatg tcctcgatgg ccttcaggag cgccggtgcc
      361 tgcgcctgcg ctgtggccgc gtcgtttcgg tccaatgcgc cccgcacttc cttgagctgc
      421 gcccggatat cgtcatgaat ggaccacatc accttgggcg gcgccgtgac cccgtactgc
      481 tccatttgtg gaaagagttc gttctctttg cggatgtaat gtttctcgat ttcggccagc
      541 cgcgcgaatt cagacgacaa ccgttccggg tcggccgtgt ccggttcgct caggagcttc
      601 cggagcagcg caatgacctc cgtgattgcg gcattttcgc ggcggaacgt gtccaccggg
      661 tgcccggaag gcgcctctac gcggctcgta cgatccagcg attcccggaa taccgcaacg
      721 tgcacgtcgc acaaacgggt gatttcttcc tcgggcagcc cttcctgaac cagctgctgc
      781 tccatcgccg cgatctcgct cgggcccacg tcctgcagga gtcgcgcaaa ttcttccttc
      841 accgcgcctg cgtccttgcc ctcatgaagc cctttgatga tctccctgag cttcgccacg
      901 cgctccgggt ccagctcggc gcccggacta cccggcgctg cctccgccgc gtcgatttcg
      961 acggtctcgc ccgtcttttc gcggatggcg ttcgcgagcg attccaacaa gccggccagc
     1021 gggacctcgc cgagctgggc agccttggcc agcgtggcaa actggcccac cgtcttgcgc
     1081 atcaacgggt tacgcaatac cttgaactta tcggacaccg aggtcagggt gtcgagcaga
     1141 aacggatact ggtcgaccag tttccccacg cgaacattgg catcgagtct catctacgga
     1201 tcctcccgga gtttcacggt ttccttgcgc cggctcccac cccggaccaa agcatagcac
     1261 gaacgccggc acagtgcatc cgttgccccc ctcggagcat ttacgggagg tcgcggcgga
     1321 agcaggcggc ctgatgcctt tcgccgcggg attcgagcgg cggcacgcct tcggcgcagc
     1381 ggggttccgc ccagggacag cggggatgga atgggcagcc aggtggcgga tttaaggggg
     1441 aggcgggttc gtcatgcagg ggaacgcgct ggcgcgtctt ttcttttccg ggatccggca
     1501 cggggatcgc gctgatcagg gcccgggtgt acggatggca cggctgatga aagactctgc
     1561 tcgcgggccc cagctccaca atccgcccca gatacatcac ggcgatccga tgagagagat
     1621 aatgtaccac cgaaagatca tgcgcgatga agagcagcgt gaggcccatc tgttcgcgca
     1681 accgggcaat cagattgagg atctgcgcct gcaccgacac atccagcgcg gacaccggtt
     1741 catcggcgat gatcagcctg ggttccagcg ccagcgcccg ggcaatggcg atccgctgcc
     1801 gctggcctcc tgaaaattcg tgagggtact tgcgcatgaa acgcgaggcc agccccaccc
     1861 gctccatcag attcgaaacg cgccgaagca gctcctgccc acgcgcgagg ccgtgcacgt
     1921 gcagcggctc cgccagcgcg tcaaacacgg tcatgcgcgg attcaacgac gcgtacgggt
     1981 cctgaaaaat cagttgaagt cgaggccgaa gcgcccggaa cgccgattcg cgcaaggcgc
     2041 tgatctcctg cccttcaaaa tacaccgttc ctccggtgca gggcgcgagg cgtaccaccg
     2101 cgcgcgccag cgtcgatttc ccgcaacccg attcgcccac gagcccgacg gtttcgcccg
     2161 gagccacatc gagactcacg ccgtcaacag cccgcaccgt cccagccagt ttccgccgga
     2221 taaggcccga atacacgggg aaatggactc ggagatcgcg cagttcgagc aggggttgcc
     2281 tcataattca ccgtcctgca cgcgaaaaca ggccgtggca tggcccggcg cgatctcctg
     2341 cagcgagatc gtaccctgac tgcagcgttc agtggcctgg ctgcaacgcg gcgcgaacgg
     2401 acagcctgta atgggggcaa tgaggtccgg agggaggccc gggatggcat gaagcggttc
     2461 gcccttgacg tgcattgagg gcagcgactg gagcaaggcc tcggtgtaag gatgccgggg
     2521 ccgcgcgaaa aggtcttccg tggcggctga ttccataacg gtgcccgcat acatcacata
     2581 agtacgccgg cacattcgcg cgaccacccc caggtcgtgc gtgatcagga gcaccgccat
     2641 gcccagctcc tcctgcagct gcgcgagcag ttcaaggatc tgcgcctgga tcgtgacgtc
     2701 gagggcggtc gtgggctcgt cggcgatgag gagctgcggc cgcgtgacca gcgccatcgc
     2761 gatcatcacc cgctgccgca taccgccgga aaactcgtgc ggccacgcgc tcaggctcct
     2821 ctcgccatcc ggaatgccca cccgccggag catctctgcc gcccgttccc gcgcgtcgtg
     2881 ctgcgagatc ttctgatgaa cgcgcaacgg ctcgatgatc tgactgccga tgcgcatgta
     2941 cgggttcaag gcggtcatgg gatcctgaaa gatcatcgag atcgcctggc cgcggatagt
     3001 ccgcagcgtc tgctcgccgc aggccagcag gtctacgccg tcaaacagcg ccgtgccctt
     3061 ctctatgcgc cccggcggcg tcgggatgag ccccatcaac gacaggctgg ccacggtctt
     3121 gcctgagccc gattcgccca caatgccgat cgtctcgccg ggttcgatgt gaaacgacag
     3181 cccatccacc gcgcggacca cgccttcgcg cgtgtgaaaa tacgtcgtca acccgtgcac
     3241 gtccagcagc ggcatgtcaa tccttcgagg cccgcacgtc gagcgcgtcg cggagcccat
     3301 cgcccaggaa attcagggaa aacagcgtga gcgacagggc cagactgggg aaaatcacca
     3361 tccacgggcg gttttccatg gtctgcacgc cctcgttgat gagcagcccc cacgagctca
     3421 tcggcgcctg tacgcccagg cccaggtaac tcaaaaacgc ttccatcagc atcacgcgcg
     3481 gcaccgtcaa cgtcgcatag acaatgatgg ggccgagcgt gttgggaatc agatgccaca
     3541 acagcacgcg ataccgcgga aggcccagga ccgtcgtcgc ttcgatgaac tctctctgcc
     3601 gcagcgacaa cacctgcgcg cggacgatcc gcgccatcgt cagccattcc accgccccga
     3661 tggccataaa cagcaatacg atattgcgcc cgaaatagac cgtcaggatg accacaaaaa
     3721 tcacaaacgg cagcgaatac agaagcgagc ccacgaccag accggcaaag gtaaaaggca
     3781 gcaagcccag ccccagggct tgggtcaact ggcctcccgg ccccgaaggc ccgagcgtga
     3841 ccagaagata aaagcccagc acggagggcg gcagcaccag cggcagggcg accacggcgc
     3901 ggaccgggac cctggcccag gatgccgtgc gggccagcca ccatgccagc ggcgtaccca
     3961 gcaacaac
//
LOCUS       DAOPBQ010000356         3968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18565_length_3968_cov_3.123435, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000356 DAOPBQ010000000
VERSION     DAOPBQ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18565_length_3968_cov_3.123435"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /locus_tag="PKW60_11075"
     CDS             complement(<1..1779)
                     /locus_tag="PKW60_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPK00018.1"
                     /translation="MHFLTIALILSAAAAEPHIYVENLSVSASSQTGGAYAVEMAIDG
                     QPATRWASADKAPMPQWFELRFTEPVELDTLLLHIPADHLYAAWKDVEVSFSSGDPVM
                     LALDAGDSRPVIRFEARPTQTVRVTIRSVHEARHYVGLFEIQAALDPDHQLDTLGAPP
                     RPKQKDDIVVRGRTEHPCVNLRPADVDAARKRCEEFEWARARRDAILEAAEPWLRESD
                     DYWLQFLPEPGAAYAYGFTGDPSTGARFGSAWSGASCTWDRPRQVRGTEGRWFPNEEY
                     PDPGTGYVAEDGRIHYFVGIYNAWVTEQWTLKALPALSEAYLLTGDERYAERGTLLLD
                     ALASIYSESTSGSWDYPSTPPSGRLARPWYQVARTLVMYVDQFDFMYHSPAMDKPSLR
                     EGMTRRENIVNHMLLDGAYYCYEHSYQGALHNGHADYVRGALAAGCLLDIPTYIDNAV
                     TSAFSIYTMLENNIDRDGRYHETALGYAIHARSLYLTFADPLYNLRNAEYPEGINLYD
                     NPKMQSTLYLPELTVMLAGRMPNFGDTAPDFKHKLMPARPMSATDYGFLERLYARTTS
                     AGQREAYGATLLWLAEGDLARLRGEQR"
     gene            complement(1868..3463)
                     /locus_tag="PKW60_11080"
     CDS             complement(1868..3463)
                     /locus_tag="PKW60_11080"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPK00019.1"
                     /translation="MRRTVLLVCFGVIVGSLGAGAAETAALDQARFLDKCRGAWAGQM
                     FGVCFGAPYEFRFNGVTVDEELQPWKPEYIAGALQQDDCYVEMTFLKALVDHGLDISY
                     EQAGQAFAKTEYPLWHANYYGRENVRAGIMPPMSGHPLYNRHADDIDFQIEADLFGIL
                     CPGLPGESNRLCDIFGRIMNYGDGLYGGMFVAGMYAAAYFEDEDVQAVLRAGLDCIPP
                     ESQYRKCIEDVIAWHKLSPDDWRATWQRIEDKWQDDIDCIPGNPFNIDAKLNGAYIAV
                     GLLYGEGDLLRTAEIATRCGQDTDCNASNATGVLGCMKGFNAMGAEFTSGIEAIRHEK
                     FIHTDYGFDTLIEACQAVTEKIIARAGGRVEGGVYHIRRQPPAPPITLEQWVHQEEVL
                     RTVITPTEMRLWNPAWRVVACGFDMDPGIRNNWGGVDKVLVLHPVSQEEPAVIEAILD
                     VPANAKELVTKVASDRRGDFLLRVIVDGELALESLIDSHGEWREVAVELAPYAGKAVP
                     VRIENVANNWQFEAAYIASIEVR"
     gene            complement(3604..>3968)
                     /locus_tag="PKW60_11085"
     CDS             complement(3604..>3968)
                     /locus_tag="PKW60_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00020.1"
                     /translation="HPAGPEEVKRAHAEALVNECASCEHVITMGDHNTRPGSPAYDIV
                     ADVLSETWLRKYPGGSGPPHPRWPESTPENRGYDVSTKRIDYIFASPDFEVLESYFVL
                     SPDSETDHPAHWSVLRCK"
BASE COUNT          752 a         1230 c         1183 g          803 t
ORIGIN      
        1 gcgctgctca ccccgcaacc gggccagatc gccctccgcg agccacagca gcgttgcgcc
       61 gtacgcctcg cgctgcccgg cgctggtggt tcttgcataa agccgctcca gaaacccgta
      121 atcggtggcc gacataggcc gtgcaggcat cagtttatgc ttaaaatcgg gcgcggtatc
      181 gccaaaattg ggcatacgtc ccgccaacat gaccgtcagc tcggggaggt atagcgttga
      241 ctgcatcttc gggttgtcgt agaggttaat gccctcggga tactctgcat ttcggaggtt
      301 gtacagggga tccgcgaaag tcaggtacag gctgcgcgca tggatggcgt agccgagcgc
      361 cgtctcgtga taccggccgt cccggtcaat gttgttctcg agcatggtat agatcgaaaa
      421 agcgctggtg accgcgttat caatgtacgt ggggatgtcg agcaggcagc ctgccgccag
      481 cgcaccccgc acataatccg cgtgtccgtt gtgcaaggcg ccttgatagc tgtgttcgta
      541 gcagtagtac gcgccgtcca gcagcatatg gttcacgatg ttctcgcggc gcgtcatgcc
      601 ttcccggagc gagggtttgt ccattgcggg actgtggtac atgaaatcga actggtcgac
      661 atacatcacg agcgtccggg ccacctggta ccagggccgg gccaggcgcc cgcttggcgg
      721 ggtgcttgga taatcccacg agccggaagt ggactctgaa tagatcgatg ccagggcatc
      781 cagcagcagt gtgccgcgct cggcataacg ttcatctccg gtcagcagat acgcttccga
      841 cagcgcgggc aatgccttta gggtccactg ttcggtcacc caggcgttgt agatgcccac
      901 gaaatagtgg atgcgaccgt cttcggccac gtagcccgtc cccgggtccg ggtactcctc
      961 gttggggaac cagcggcctt cggtaccccg gacctgtcgg gggcggtccc aggtgcacga
     1021 ggcgccggac caggcactgc caaaccgggc gcccgttgac ggatcgcccg tgaatccata
     1081 ggcataggcc gcgcccggct cgggaagaaa ctgcagccag taatcatccg attcgcgcaa
     1141 ccagggctcc gcggcctcga ggatggcatc ccgcctggct ctggcccatt cgaattcttc
     1201 gcaccgcttc cgcgcggcat ccacgtccgc cggcctgagg ttcacgcagg gatgctctgt
     1261 ccggccgcgc accacaatgt cgtccttttg tttcggccgc ggaggagcgc caagggtatc
     1321 gagctggtga tcggggtcca gggccgcctg aatctcgaaa aggccgacat aatgccgggc
     1381 ttcatgcacc gagcggatcg tcacgcgcac tgtctgcgtt gggcgcgctt caaaacggat
     1441 gacaggacgc gaatcgccgg cgtccagggc caacatcacc ggatcaccgc tggaaaagga
     1501 gacttcgacg tccttccatg ccgcgtacaa atgatccgcg gggatatgca ggagcagggt
     1561 atccagttcg acgggttcgg tgaatcgcag ctcgaaccat tgcggcatag gagccttgtc
     1621 cgcgctggcc caccgggttg cgggctggcc atcgatcgcc atttccaccg cgtatgcgcc
     1681 cccggtttgc gacgatgcgg acacgctgag gttttccaca tagatatgcg gttccgccgc
     1741 cgctgccgag agtatcaggg cgatggtcaa aaagtgcata ccggcgttct cctgtcgtgg
     1801 gcatttcggc tgggcaaccg caactcacag cgcaggtgca gggggacggt tgcaccgtaa
     1861 gataagctta ccgcacctcg atcgaggcga tgtaggccgc ctcgaattgc cagttgttgg
     1921 cgacgttttc gatgcgaacc gggacggcct tgcctgcgta gggggccagc tcgacggcca
     1981 cctccctcca ctccccgtgg ctgtcgatga gcgactcaag cgccagttct ccgtctacaa
     2041 ttacccggag cagaaaatca ccccggcgat ccgatgccac ttttgttaca agctccttcg
     2101 cgttggcagg cacgtcgaga atggcctcaa ttacggccgg ttcctcctgg cttacgggat
     2161 gcaggaccag aaccttgtct acaccgcccc aattgttccg aatccccgga tccatatcaa
     2221 aaccacaggc gacaacgcgc catgcggggt tccacaggcg catttcggtg ggtgtaatca
     2281 cggttcgcag cacttcttcc tgatgcaccc attgttctag cgtgatggga ggcgcaggcg
     2341 gttgccgccg gatatggtac accccgcctt caacgcgccc gccagctcgt gcaatgattt
     2401 tctctgtgac ggcctggcac gcctcgatca gcgtgtcaaa gccgtagtcg gtatggataa
     2461 atttctcgtg ccggatcgct tcgatgccgg aggtgaactc ggcccccatc gcattgaagc
     2521 ccttcataca gcctaaaacg cccgtcgcgt tggaggcatt gcaatcggtg tcctggccgc
     2581 agcgcgtggc gatttcggcc gtgcgcaaca ggtcgccttc cccgtaaagc agtcccactg
     2641 cgatataggc gccgttgagt tttgcgtcaa tattgaatgg attccccggt atgcagtcga
     2701 tatcgtcctg ccatttgtcc tcgatccgct gccacgtagc ccgccagtca tcgggcgaaa
     2761 gcttatgcca ggcgatcacg tcctcgatgc atttccgata ctgcgattca ggcggtatgc
     2821 agtccagtcc agctcgaaga acggcttgaa cgtcttcatc ttcaaaatat gccgccgcat
     2881 acatccccgc cacgaacatc ccaccataca ggccatcgcc gtagttcatg atccgtccga
     2941 agatgtcaca caggcgattc gattcaccgg gcagcccggg gcacaagata ccgaagaggt
     3001 cggcctcgat ctggaagtcg atatcgtcgg catgacggtt gtacagtgga tgaccggaca
     3061 tcggcggcat gatacccgcc cgcacgtttt cccggccgta gtagttggcg tgccagaggg
     3121 gatattccgt ttttgcaaag gcctgcccgg cctgttcgta cgagatgtcc aggccgtggt
     3181 ccacaagtgc cttcaaaaac gtcatttcca catagcaatc gtcctgctgg agggcgcccg
     3241 cgatatattc cggcttccag ggctgcaact cttcatcgac ggttaccccg ttgaagcgga
     3301 actcgtacgg tgcgccaaaa catacgccga acatctgccc ggcccacgcg ccgcggcatt
     3361 tgtccaaaaa ccgggcctgg tcgagcgcgg ccgtttccgc cgcaccagcg cccaggcttc
     3421 ccacgataac gccaaaacaa acaagcaaga ctgtcctgcg catgctacgt cctcctggtt
     3481 gcgtgcccaa acgtatcgtg gggttttccg cgcggaaaat caagactgct cgaggccgga
     3541 gcccctcgtt cacggggttg aaatcggggc taatccccgc cctcccgttt caccgcccgg
     3601 ggctcatttg caccgcaaga cggaccagtg ggccgggtgg tcggtttctg aatcgggaga
     3661 aagcacaaaa tatgactcga gaacctcgaa atcgggtgat gcgaatatgt agtcgatgcg
     3721 tttcgtgctg acgtcgtagc cacgattctc gggcgtactt tccggccagc gcgggtgcgg
     3781 cggtccactg ccgccggggt actttcgcag ccaggtttcc gacagcacat ccgccactat
     3841 gtcataggcg ggactgcccg ggcgcgtgtt gtggtcgccc atggtgatca cgtgttcgca
     3901 tgaggcgcat tcgttcacga gggcctcggc gtgggcgcgt ttaacctcct ccgggccggc
     3961 cgggtggt
//
LOCUS       DAOPBQ010000357         3963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18588_length_3963_cov_4.128710, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000357 DAOPBQ010000000
VERSION     DAOPBQ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18588_length_3963_cov_4.128710"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="PKW60_11090"
     CDS             complement(<1..1565)
                     /locus_tag="PKW60_11090"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HPK00021.1"
                     /translation="MTELFFESYEIPAADLGGENPLPVFRGNSDDLEIRLADNVSAEE
                     RRHIGRALASRVLPYGMQDEYTRARRPRVFQTLILENECLRARFSPELGGRLLSLFDK
                     TDGRELLERNPVFQPCNVAIRNAWISGGVEWNTSHLGHYFLTVSPVYAARIRGVHGEP
                     ALRLYEWDRLHCFPWQIDFFLPEGSRFLFARPRIINPHAYTLPMYWWSNIAVPEHPER
                     RTLVPAADALSHPPGTGVLTVMPMPRPAGYDITYPTNAPGAGEAFFRIPAEQRKWVAQ
                     VDREGKGLVHASTSRLMGRKMFFWGMNPGGRRWQEQLSADGHAYIELQGGLARTQLET
                     VPMPGGEQWSWTEAYGLLSVAPEIAHGEDWEQAWRAADAALEQVLPREELDALHARCE
                     SCASAAPEQVLARGAGWGALELRRAAQIGQQPGIPPELPFPDAAIGPDEEMWLSLLER
                     GALTAREPGQGPGAYMIQPEWRTILEASVRNSGGGDWLSCLHLGVMRMEARESGGARE
                     AWEQSIAHTPTAWA"
     gene            1856..2797
                     /locus_tag="PKW60_11095"
     CDS             1856..2797
                     /locus_tag="PKW60_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HPK00022.1"
                     /translation="MYRPEIRVLDCTIRDGGLMNGWRFDKEMVKSVFAGLAEAGIDYV
                     ELGYRADKNQFSKDAFGPWRFCDEADLREVAFECRTKVAVMADVGRTDYNDIVPRDQS
                     IIRLYRVATYVKDIVKAIELGNHCKENGYEVSINIMAISHALEPDLDRALDQLRDTPF
                     DMVYLVDSFGYLEPRQIEYMAHKYLTRLPGKQIGIHCHNNQQLAFANTVEAAEKGITF
                     LDGSIYGIGRAAGNCPLELILGFLKNPRFDVRPVLDLIQTYFLPLKQNLKWGYEIPYA
                     VTGLLNKHPRAAMAFMDGAHGDTFRAFYDELSNVDDV"
     gene            3110..3538
                     /locus_tag="PKW60_11100"
     CDS             3110..3538
                     /locus_tag="PKW60_11100"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPK00023.1"
                     /translation="MARYDIHDRWEDPYEPRQERRVARRFAAPLQVKIAVHDSKLKCR
                     LVCRGNVMNVSRVGALVRTRHQVEPGVRLSIAIPTKICKDSVCLPKVFVGSAEVIRAT
                     EEGDGISVVALRFGTELAQNMEFALFTDALHGQFFPRVEV"
     gene            3693..>3963
                     /locus_tag="PKW60_11105"
     CDS             3693..>3963
                     /locus_tag="PKW60_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00024.1"
                     /translation="MLALLLFAATQCVAQPQTFTLETTEPVWDIAVEDLDLNGGADIL
                     ALCCDPETDPLRKNLSVFMSGDDGGYPSVPSFVLPLDPGISALFLA"
BASE COUNT          808 a         1160 c         1213 g          782 t
ORIGIN      
        1 gcccacgcgg tgggagtgtg ggcgatgctt tgttcccagg cctcacgggc gccgccgctt
       61 tcacgcgctt ccatgcgcat gacgccgaga tgcaggcagg acagccagtc cccgcctccg
      121 ctgtttcgca cgctggcctc caggatggtc cgccattccg gctgaatcat gtaggcgccg
      181 ggcccctggc cgggctcgcg agccgtcagc gccccgcgct cgagcagcga gagccacatt
      241 tcttcgtcgg ggccgatcgc cgcatctgga aatggcaatt ctggaggaat gccgggctgc
      301 tgcccgatct gggctgcgcg gcggagctcg agcgcacccc agcctgcgcc acgggccagt
      361 acttgttccg gcgcggcact cgcgcaggac tcacaacgtg cgtgaagggc gtcgagttcc
      421 tcgcggggca acacctgttc cagggcagca tcagccgcac gccaggcctg ctcccaatcc
      481 tcgccgtggg caatttcagg cgcaacagaa agcagcccgt aggcctcggt ccacgaccac
      541 tgctcgccgc cgggcatggg taccgtttcg agctgggtgc gggcgagccc gccctgcagc
      601 tcaatgtagg cgtgcccgtc tgcggagagt tgctcctgcc agcgccggcc gcccggattc
      661 atgccccaga aaaacatctt tcgccccatc aggcggctgg tggaggcatg aaccagccct
      721 ttcccctccc gatccacctg cgcgacccat ttgcgctgtt ccgccgggat ccgaaagaag
      781 gcttcgccag cgcctggggc attggtggga taggttatgt cataacccgc cggacggggc
      841 atgggcatga cggtcagaac gcccgtgccg gggggatgcg acagggcgtc cgcggccggc
      901 accagcgtgc gccgctccgg atgctcgggc acagcgatat tggaccacca gtacatcggc
      961 agcgtgtatg cgtgcggatt gatgatcctg gggcgcgcga atagaaaacg ggagccttct
     1021 ggaagaaaga agtcgatctg ccagggaaag caatgaagcc ggtcccattc atacagccgc
     1081 agcgccggct caccgtgcac accccggatg cgcgcggcat agaccggcga caccgtcagg
     1141 aagtaatgcc cgaggtgcga ggtattccat tccaccccgc cgctgatcca ggcatttcga
     1201 atcgcgacat tgcacggctg aaataccggg ttgcgctcga gcagttcgcg gccgtcggtc
     1261 ttatcgaaca gcgacagcag gcgcccgccc agctccggag aaaagcgcgc tcgaaggcat
     1321 tcgttctcga ggataagcgt ctggaacacc cgaggccggc gcgcccgggt gtattcatcc
     1381 tgcatgccgt aggggagcac acggctcgcc agggcgcgcc cgatgtggcg gcgctcttcc
     1441 gcggagacgt tgtcggcgag cctgatctcg agatcgtccg agttccctct gaagacgggc
     1501 agtggatttt cgccgcccaa atccgccgcc gggatttcat acgactcaaa aaaaagttca
     1561 gtcatgcgta cctcgatggg tggttctatg cgcccgggcc catgcggccc aacctccaga
     1621 agcggtctgc actacagttg atgcgcgcct ttacggcggg tgcgcttgct gcattacacc
     1681 cgctggcgca agctggattc tacctggatt gccgcgctgg atcgactgcg cggagcaccc
     1741 aacggccagc gcgcacagcc gatggaggct cactgctgca ctaccgggaa attgatccat
     1801 cggaatgctt tcggtacgct ggtacattcc tttgacattg acagggagaa gcgtgatgta
     1861 caggccagag atccgggtac tggattgcac cattcgcgat ggcgggctga tgaatggctg
     1921 gcgctttgac aaggagatgg tcaagagcgt atttgccggc ctggccgagg cgggcatcga
     1981 ttacgtcgaa ttgggctatc gtgccgataa aaaccagttc tcaaaagacg cgttcgggcc
     2041 gtggcgattt tgcgacgaag ccgacctgcg cgaggtggct ttcgaatgca gaaccaaggt
     2101 cgcggtgatg gccgacgttg gccggacaga ctacaacgac atcgtgcccc gcgaccagtc
     2161 gataatccgc ctgtaccgtg tcgcgacata cgtgaaggac atcgtcaaag cgatcgagct
     2221 tggaaatcac tgcaaagaaa acgggtatga agtctcgatc aatatcatgg cgatatccca
     2281 tgcgctggaa ccggatttgg acagggcgct tgatcagctg cgtgacaccc cgtttgacat
     2341 ggtctacctg gtggacagtt tcgggtatct cgagccgcgc caaattgaat acatggcgca
     2401 caaatacctc acccgcctgc ccgggaagca aatcggcatc cattgccaca acaatcagca
     2461 gctggccttt gcaaacaccg tggaagcggc ggaaaagggg atcacgttcc tggatggctc
     2521 gatctatggc atcggccgcg ccgccggcaa ctgccctctg gagctgatcc tgggctttct
     2581 gaagaatccg cggtttgatg tacgacccgt tctcgacctg attcagacgt attttctccc
     2641 gttgaagcag aatttgaaat ggggatatga gataccctac gccgtaaccg gtctcctcaa
     2701 caaacaccct cgtgccgcaa tggcgttcat ggacggggct cacggcgaca cgttccgcgc
     2761 cttctacgac gagctgtcga atgttgacga tgtttagttg acgaaaatca cgcccgattg
     2821 gcagttacaa gccctctgca caccggaatg acagattcaa gcctctctga caatgcaagt
     2881 tacgttaaaa atttcagaat atgcgaatat tgtgaaaagt cgcccactcg agtgaatgca
     2941 gagatgacct taaaatatga caaatacgta aagcgcaatt tttgctagac aaaagtgtta
     3001 ataaatggta gagtaggtgc aggagaaggg tgtaaaggcg aatgcccgca tcgtaaagac
     3061 cgggaggagc gtagcagacg acacttttga agcaaggagt ggcatgttga tggcgcgata
     3121 tgacatacat gaccgatggg aagacccgta tgagcctcgg caggagcggc gggtagcgag
     3181 gcgctttgcc gcaccgcttc aggtcaaaat cgcggtacac gattccaagc tgaagtgccg
     3241 gctggtatgc cgtggcaatg tgatgaatgt ctcccgggtg ggtgcgctgg tccgcacacg
     3301 gcaccaggtg gagccgggag tgcggctttc gatcgccatt cccaccaaga tctgcaaaga
     3361 cagtgtgtgc cttcccaagg tgtttgtggg ttctgctgaa gtcattcggg cgactgagga
     3421 aggcgacggt atttcggtcg tggcgctgcg ttttggaacg gaactggccc aaaacatgga
     3481 attcgccttg tttactgacg cgctgcacgg ccaattcttc ccccgcgtcg aggtttagag
     3541 caccacgcag gcgcgccgtt tctccgcggc acctcctccg cgccgggctg cgctgtctgt
     3601 gtaaaattcc cccgttggcc gggtgatgaa tgacaccgcc gtattctcgt caggtatact
     3661 gtgtctcaat actcaatcat ctgggagagg ccatgcttgc attattgctg tttgcggcca
     3721 cacagtgcgt cgcgcaacct cagaccttta cgctggagac tacggaaccg gtatgggaca
     3781 tcgccgtcga ggatttggat ctgaacggcg gcgcggacat ccttgcgctg tgctgcgatc
     3841 ccgagacgga tccgcttcgc aagaatctgt cggtgttcat gagtggggac gatggcggct
     3901 acccgtctgt gccgtcgttc gtgcttccgc tggatccggg catcagcgcg ctcttcctgg
     3961 cag
//
LOCUS       DAOPBQ010000358         3959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18613_length_3959_cov_3.663678, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000358 DAOPBQ010000000
VERSION     DAOPBQ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18613_length_3959_cov_3.663678"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..2543)
                     /locus_tag="PKW60_11110"
     CDS             complement(210..2543)
                     /locus_tag="PKW60_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00025.1"
                     /translation="MTIKFSQVLSVVCVILVAGAWPAGAATETARHWKPVADVPYLQE
                     VGSQIPAEQPVTSLAALSDTVYAVMGGEVHVIRDGALEKSAGAPEGVRRLRSLDGALW
                     AVAESGAYRFTGDAWQHVDERSFVDFCLHLGAVYGATRNEIFRYQDGAFVSATPEGGY
                     LSSDSTMVMEDFSQVLADPVRIGPIDRIASYSGTMYILRPGGLALIDIDTFVPEPIDW
                     GTLPSRATRDMLVQGSRLYVTTDRGLAVLRGMAMTVVHGADGLPYEDTTCLAEGFDGD
                     LWIGTTRGAIRRTGSDYHYFGAEHWIPGDNVFDIVVAGKTVYIATDKGLGIIRYEPYT
                     LAKKAAYFERALEEWGHKRLGFVHYLYWGGEQDGWLREISDNDGGNTTHYLAAMSFKY
                     AATGDEQARQEALNAFEAMIWLDDITPKPGFIARAIWSVQGDKGRRSERGSGGLPAKW
                     YPTEDGLWQWKGDTSSDEVNGHIYSVSLFHDLAAKGPEKDRARKHIANIASHIMDNGW
                     VLRDMDGKPTRWGRWDPDYLLKPYGYEARGLNGMEAQTYVITAHALTGDSKFSQGLDQ
                     LLQWRYHRYTVRQKLTFPPDQIVTWDDELAFRCYHPLLTYAQDPALRSVYLRSLERSW
                     EVMRMQHVAFFNFIYGALTGNDCEEERAAQHLREWSLDTVNRSYTNSHRSDLHPEPGY
                     VPYAGGTRAMSPRETEAMGGSRSALRLDGRDGGRAVTPPIGWLEDYWMGRYYGLIEAP
                     AVSDPALISVEAGPLRQLGAAPYDGPPRPTGLIPGES"
     gene            2753..3760
                     /locus_tag="PKW60_11115"
     CDS             2753..3760
                     /locus_tag="PKW60_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPK00026.1"
                     /translation="MRDRYSYTRISRKRVREAASGYLFIAPWMAGFLLFTLGPMTASA
                     ILSFCRYDPSAVPSRLEWVGLSNYIRMFTGDPLFWKSLSVTLRYSLISIPLQLAGGLA
                     LALLLNRHVRGISIYRTAFYLPMVLGGVAISLLWLWLFSPSQGLINIGLAKVFDFLGQ
                     DSLMAHLLAWPLQALFGKAGADSLLPGWISSERGALNSLILMSCWSLGGSMIINLAGL
                     QAIPRTYYEAAEIDGAGRWTQFWRVTIPLLSPTIFFNLVMGVIGSFQVFTQGLIMTAG
                     GPNNSTCFYVLNTYRNAFEFFRMGYASALAWFLFLIILSLTLLVVRSSSMWVFYEAKR
                     R"
     gene            3757..>3959
                     /locus_tag="PKW60_11120"
     CDS             3757..>3959
                     /locus_tag="PKW60_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00027.1"
                     /translation="MTGQVRKSRRALRRWLAPLITHAILIAATVVMLLPLAWMISTSL
                     KESGIGLSRGIEWLPWRDTWTAGG"
BASE COUNT          730 a         1205 c         1206 g          818 t
ORIGIN      
        1 aaagagtgca caacttatca ctgtatgtca gagatgacaa caatttactc aggatgactc
       61 aatcctgatc ggaggaaaat tacgcccaaa tggagcccgg tggctccgaa cctgggattt
      121 cactgaaatt tggcctcatc ccggaaacct gcacattttg acacaccctg ggactgccgc
      181 ttcggcagtc ccagggtatt ggaacagggt caggattcgc cggggatcag gcccgtggga
      241 cgcggcgggc cgtcgtacgg agcggcgccc agctggcgca aggggccggc ttccacggag
      301 atcagggccg gatcggatac cgcaggcgct tcaatcaagc cgtaatagcg gcccatccaa
      361 taatcttcca gccagccgat cggcggcgtg acggctcgcc cgccgtcgcg gccgtctaac
      421 cgcagcgcgc tgcggctgcc gcccatggcc tcggtttcgc gaggcgacat ggcccgtgtg
      481 cccccggcgt acgggacgta accaggctca ggatggaggt cgctccggtg cgagttcgta
      541 tagctgcgat tgaccgtgtc gagcgaccat tcgcgcaggt gctgagcggc ccgctcttcc
      601 tcgcaatcgt tcccggtaag ggcgccgtag atgaagttga aaaacgccac gtgctgcatg
      661 cgcatcactt cccagctgcg ctccaggctg cgaaggtaga cggagcgcag ggcgggatcc
      721 tgggcatagg tgagcagcgg gtgataacac cggaacgcga gttcgtcgtc ccaggtcacg
      781 atctggtccg gcgggaacgt gagcttctga cggacggtgt atcggtggta gcgccattgg
      841 agcagctggt cgaggccctg agaaaacttg gagtcaccgg tcagggcgtg cgcggtgatc
      901 acgtaggtct gcgcttccat gccgttcagt ccgcgcgcct cataaccata cggtttcagc
      961 aaatagtcgg gatcccagcg cccccagcgg gtaggtttgc cgtccatgtc gcgcaatacc
     1021 cagccgttgt ccataatgtg cgaggcaata ttcgcgatat gcttccgggc acggtctttt
     1081 tcggggcctt tcgcggccag gtcgtgaaac agggacacgg aatagatgtg cccgttgact
     1141 tcgtcgctgg acgtgtcccc tttccattgc cagaggccat cctcggtggg ataccatttc
     1201 gcgggaaggc cgccggagcc tcgttccgac ctccggccct tgtcgccctg gacagaccag
     1261 atggctcgcg cgataaaccc gggtttgggg gtaatgtcat cgagccagat catggcttca
     1321 aaggcgttga gggcctcctg gcgggcctgt tcgtcgcccg tggccgcata cttgaacgac
     1381 atggcggcca gataatgggt cgtgttgccc ccgtcattat cgctgatctc acggagccag
     1441 ccgtcctgtt cgccgcccca gtagagataa tggacaaatc cgagccgctt gtgcccccat
     1501 tcctcgaggg cccgctcgaa gtatgcggcc ttcttggcga gcgtgtacgg ctcgtagcgg
     1561 atgatgccca gtcctttgtc tgtggcgata tagacggtct taccggccac cacgatatca
     1621 aacacgttgt cgcccgggat ccagtgttct gcgccaaaat aatgatagtc gcttccggtc
     1681 cttcgtatcg cgccgcgcgt ggtgccgatc cagaggtcgc cgtcaaaacc ctcggccaga
     1741 caggtggtgt cttcataggg caatccgtcc gcgccgtgca ccacggtcat cgccataccg
     1801 cgcagcaccg ccaggccgcg atccgtagta acgtaaaggc ggcttccctg gacgagcatg
     1861 tcgcgcgtgg cccgcgacgg caaggtcccc cagtcgatgg gctccggaac aaaggtgtcg
     1921 atatcaatca gcgcgagacc gcccgggcga aggatataca tggtgccgct gtacgacgcg
     1981 atgcggtcaa tcgggccaat gcgcaccgga tcagccaaca cctgcgaaaa atcttccatc
     2041 accatggtcg aatcgctgga aaggtacccg ccttccggcg tggcactcac gaaggccccg
     2101 tcttgatagc gaaaaatctc gttacgcgtg gctccataaa ccgcgcccag gtggaggcag
     2161 aaatcaacaa acgagcgctc atcaacgtgc tgccaggcgt cgccggtgaa gcggtaggcg
     2221 cccgactcgg caacggccca aagggcgcca tccagggagc gcagccggcg aacgccttcc
     2281 ggtgcaccgg cggatttttc gagcgcgccg tcgcgtatca cgtgcacctc accgcccatg
     2341 acggcataga ccgtatcact cagtgcggca agagaggtga cgggctgttc ggccggaatt
     2401 tgactgccga cttcctgtaa ataggggaca tcggcaacgg gtttccagtg gcgggccgtt
     2461 tccgtggcgg cccccgccgg ccacgccccc gcaacaagta tcacgcaaac cacacttaga
     2521 acctggctga acttgatggt catggccctc gctcccttcc atacctgcgc accagaacag
     2581 gttgtttcgg aatgtgtgag gccatgatgg cgttgacggg cggcggtgtc aagacggata
     2641 tcgaatgacg cgggcgatct gatgttcgat ttgccgcacc gccatctcat ttctatacta
     2701 tgcgggtttg agcaggtgcc gccaggccga aaaatactcg tgggactgca tcgtgcgtga
     2761 tcgctattca tatacccgaa taagcaggaa acgtgttcgg gaggccgcat ccggctatct
     2821 gttcattgcg ccctggatgg caggtttcct gctcttcacg ctcgggccca tgacggcctc
     2881 ggccatcctg agcttctgcc gctacgaccc ctcggccgtg ccgagcaggc tcgaatgggt
     2941 tggtctgtcg aattacatcc ggatgtttac cggggacccc ttgttctgga aatcgctgtc
     3001 cgtcacgctt cggtactcgc tgatcagcat tccgcttcag cttgcagggg gacttgcgct
     3061 ggccctgttg ctgaaccgcc acgtacgggg tatttcgatc taccgcaccg cgttctatct
     3121 gcccatggtg cttggcggcg tagccatttc gctgttgtgg ctgtggctgt tcagccccag
     3181 ccagggactg atcaatatcg ggctggccaa agtctttgac ttcctcggac aggacagcct
     3241 catggcgcac ttgctggcct ggccgctcca ggcgctgttt gggaaggccg gcgcagacag
     3301 cctgctgccg ggctggatct cgagcgaacg cggcgcgctc aattcgctca tcctcatgag
     3361 ttgctggtcg ctgggcggtt cgatgatcat taatctggcg gggcttcagg cgatccccag
     3421 gacctactac gaagcagcgg agatcgacgg cgcggggcgc tggacccaat tctggcgcgt
     3481 tacgattccg ctgctgtcgc ccacgatctt ttttaatctc gtcatgggcg tcatcggctc
     3541 gtttcaggtg ttcacgcagg gcctgatcat gacggccggc ggccccaaca acagcacctg
     3601 cttttatgtc ctcaatacct accggaacgc atttgagttt ttccgcatgg gttacgcctc
     3661 agccctggcg tggtttttgt ttctgatcat cctgtccctg acacttctcg tcgtgcggag
     3721 ctcgtccatg tgggtcttct acgaggcgaa acgccgatga cggggcaggt ccgaaaatct
     3781 aggcgcgcac tgcggcgctg gctggcgccg ctcatcacgc atgccattct gattgcggcc
     3841 accgtggtaa tgctgctccc attggcctgg atgatttcca cgtcgctcaa agagagcgga
     3901 atcgggttat cacgcggcat tgaatggctg ccctggcggg atacctggac ggccggcgg
//
LOCUS       DAOPBQ010000359         3955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18628_length_3955_cov_3.447949, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000359 DAOPBQ010000000
VERSION     DAOPBQ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18628_length_3955_cov_3.447949"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..1552
                     /locus_tag="PKW60_11125"
     CDS             362..1552
                     /locus_tag="PKW60_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00028.1"
                     /translation="MKINRRGFLASTAAIPFVSQCAAQPENIKYRACIIGDTHEGGYG
                     HSLHLAFAHRPDVQVVALADPDEDGRARHAAEAKAQRTYADYREMLEKEKPDLVSVAP
                     RTTVRHREYVLACAEHACHGFLEKPIATDLAEADAMVAAVEAKNLKWAMAHNYRMSPH
                     IQHMKKMLLEERLIGHIIEIRSHGKEDNRAGAEDMIVLGSHTFDMMQFLMGPPEWCMA
                     DITVNGKPAQPSDVKEATEPLGPIVGNRINAVFGLGKGIAGFFGSIVQPDGNGGRWGL
                     DIYGSKGIIQARFDDPKKTDGIPTVTYLADPSWAPGMTGQGWAPLPGAPEVVMKNPEN
                     DRNAPIIDDLIASIEEDRRPQTSLQDGRTAYEFIQGVFAAHIHGGRVALPLATRTHPL
                     KGWA"
     gene            1700..1939
                     /locus_tag="PKW60_11130"
     CDS             1700..1939
                     /locus_tag="PKW60_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00029.1"
                     /translation="MVSRKWLRIALLCLVLCYMLAYLGEVAFAASNTDLAEKKGIEGL
                     FKKKDTAADPRAPNKTQKFVGVASFFVMIVVVKYL"
     gene            2071..3438
                     /locus_tag="PKW60_11135"
     CDS             2071..3438
                     /locus_tag="PKW60_11135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPK00030.1"
                     /translation="MATIPDDTTGQAVSRILILDDEPGILEVLSQHLASAGYACTSTT
                     SPARALDLLRNERFSLLLTDLRMPEMDGLEVVQRARAIDSELSIVVVSALTEVTQAIQ
                     CMRLGAEDYVLKPFNLEDISRSVNRAVEKQRAVTESHEYQERLRQRVDSATEELERVN
                     QELRETKQYLENLLESTVDGIITIGKDETIEFANGGALAMVGFRKETFLGLPIGRLLA
                     GGEDEFINIQRLLNQYDYLKNYETHILTQSGGRIPVNISFSRVDDARGEFASTLAICK
                     DITEQKRLEQELMEMSVKDSLTGLYNQRSFFERLENEVERAKRQKRPLSLLLFDIDKF
                     KQYNDAWGHLEGDKVLRGAGNAVLECTREHVDIGFRYGGDEFTVILPEAEEPQAFAIA
                     ERIRETFQKYRFDDLTLSIGCVFYQEEHTVRSLIEKADALMYEAKRAGGNRVCSPGVP
                     GKARK"
     gene            3465..>3955
                     /locus_tag="PKW60_11140"
     CDS             3465..>3955
                     /locus_tag="PKW60_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPK00031.1"
                     /translation="MKFAICNEIFQEWNDIARTFNYVKEIGYDGIELAPFTFSQYVTD
                     ISPETRKEIVNRAREVDLDVIGIHWVFVGPEGLHINHPDKEHRDRAAQYLIDLAQFCG
                     DVGGRVMIFGSPKQRNVHEKLTYDQAFEYTAAVFEQALPACEKNGVTICMEPLTHLET
                     NFCT"
BASE COUNT          899 a         1165 c         1138 g          753 t
ORIGIN      
        1 catacccgta acagctcatg tgatcgcggc gcaggctgtc gatcgtgaac agtacgatat
       61 tcggatgctt cgcagccacg tatcttcctc catacccagt ctcacgaccc cacacggcgg
      121 tgttcgccag cgctcgaggc gggggtgctg ggcacgtttg cgccatagta acgaaaagca
      181 tgtgcgtgcg tcacgggaca gagacacatt cactccctgc gtgaggcagg catttccatt
      241 ttgcgttgac attatttggc acgggcttga cgcaagggcg cggcacatgt gacgattagc
      301 tttgacatgc tgccgaggcg ggatgcgggc agtcgcccgt tccacaaaga ggatgcacat
      361 tatgaaaatc aaccggcgtg gatttctggc cagtacggcc gccatcccgt tcgtctctca
      421 atgcgcggcc caacctgaaa acatcaaata ccgggcctgc attattggcg atacccatga
      481 aggagggtac ggccactccc tgcaccttgc ctttgcccac cggcccgacg ttcaagtcgt
      541 cgctctggcc gacccggacg aggacggccg cgcccggcat gccgctgaag ccaaggcaca
      601 acgcacctat gccgactatc gcgagatgct tgaaaaggaa aagccggacc tggtctcggt
      661 tgccccgcgc accacggttc gccaccgcga atacgtgctg gcctgcgccg aacacgcgtg
      721 ccacggtttt ctcgaaaaac ctatcgctac cgacctggcc gaagccgatg ccatggttgc
      781 tgccgtcgag gcgaaaaacc tgaaatgggc catggcccac aattaccgca tgtcgccaca
      841 tattcagcac atgaaaaaaa tgctccttga ggagcgcctc atcggccata tcatcgaaat
      901 tcgcagccac gggaaagagg acaatcgagc gggcgcagaa gatatgatcg tcctgggctc
      961 ccacaccttc gatatgatgc agttcttgat ggggccgccg gaatggtgca tggccgatat
     1021 cacggtaaac ggcaaacccg ctcagccgtc cgatgtaaaa gaggccaccg aaccgctcgg
     1081 gcccatcgtc ggcaaccgca tcaacgccgt gtttggtctg gggaagggca tcgcgggctt
     1141 tttcgggtcg atcgtccagc ccgatggcaa tggcggccgg tggggcctcg acatttacgg
     1201 ttcgaagggc atcatacagg cccgcttcga tgatccgaaa aagaccgatg gcatccccac
     1261 ggttacttat ctggccgacc cgtcctgggc gccgggcatg accggccagg gctgggcgcc
     1321 tttgccaggt gcgcccgagg tcgtcatgaa aaacccggaa aacgaccgga atgcccccat
     1381 cattgacgat ttgatagctt ccatcgaaga agaccgccgg cctcagacca gtctgcagga
     1441 cgggcgtacg gcctacgaat tcatccaggg ggtgttcgcg gcccatatcc acggcggccg
     1501 cgtcgcgctg cctctggcca cgcgcaccca tccgcttaaa ggctgggcgt aagctgttga
     1561 ttatttgcca cttgtaagga cggagctttt cagcgtacaa tctaggtggc ttggggcagc
     1621 ccggtcagat gcgcgggacg cccgggactc gcctgcaggc gagccgggtg gagctggttg
     1681 gaatgaggag attgaacaga tggtgagccg caagtggtta cgaatcgccc ttctgtgcct
     1741 cgtactgtgt tacatgctgg catacttggg cgaggttgcc ttcgccgcta gtaatacgga
     1801 tttggccgag aaaaaaggca tcgaaggcct gttcaaaaag aaagacacgg ccgccgaccc
     1861 acgagcacca aacaaaaccc agaaatttgt cggggtcgct tcgtttttcg tgatgatcgt
     1921 tgtcgtaaaa tacctgtagc ccaatcccgc gcgctccgtg cgtgggagtg cgggaatacg
     1981 caacggagaa caggaccgga gccccgctgg tgggcccggt tacggaattt ccactgggac
     2041 agcagaggtg gtggaggacg ggaaaaatcg ttggctacca taccggatga taccactgga
     2101 caggcggtga gccgcatcct gattcttgac gatgaacccg ggattctgga agtgctgtcc
     2161 caacacctcg ccagtgcggg ctatgcatgt accagtacca catcgcctgc ccgcgccctg
     2221 gacctcctcc ggaatgaacg tttctcgctg ctgctgaccg acctgcgcat gccggagatg
     2281 gatgggctcg aagtcgtgca gcgcgccaga gccatcgact cggagctgtc gatcgtggtg
     2341 gtctcggccc tgaccgaggt gacccaggcc attcagtgta tgcgcctcgg cgccgaggac
     2401 tacgtcctca aaccgtttaa ccttgaagac atctcccgat ccgttaaccg ggccgttgaa
     2461 aaacagcgcg cggttaccga atcgcatgaa tatcaggagc ggcttcgcca gcgcgtcgat
     2521 tcggccacgg aggaactgga gcgggtcaac caggagcttc gagaaacgaa gcaatacctg
     2581 gaaaatctcc tggaaagcac ggttgacggc atcatcacga tcggaaaaga cgagacgatc
     2641 gaatttgcca atggcggcgc gctcgcgatg gtcggttttc gaaaagagac ctttcttggc
     2701 cttcccatcg gccggctcct ggcgggcggc gaagacgaat tcatcaatat ccagcgcctc
     2761 ctcaatcaat acgactatct caaaaactac gaaacacata tcctcaccca aagcggcggg
     2821 cgcatcccag tcaacatctc cttttcccgc gtggacgatg cgcgcgggga gtttgcctcc
     2881 acactggcta tctgcaagga catcaccgaa cagaagcggc tcgaacagga gctgatggag
     2941 atgtccgtga aagactcgct gacgggcctg tataaccagc ggtcattttt cgaacgcctt
     3001 gaaaacgagg tagaacgcgc caaacgccag aaacgcccgt tgtcgctgct gcttttcgat
     3061 atcgacaagt tcaagcagta caacgacgcc tggggccatc tcgaaggcga caaggtgctg
     3121 cgtggcgccg ggaacgcggt gctcgaatgt acacgagagc acgtcgatat cgggtttcgg
     3181 tatgggggtg acgagttcac ggtcatcctt ccggaagcag aagaaccgca ggctttcgcg
     3241 atcgccgaac gcattcgcga aaccttccag aaataccgct ttgatgactt gaccttgagc
     3301 atcggctgcg ttttctatca ggaagagcac accgtccgtt cacttatcga aaaagccgat
     3361 gccctgatgt atgaggccaa acgcgccggc ggcaaccggg tctgcagccc cggcgtgccg
     3421 gggaaagcgc ggaaataacg atccgaaaag ggagccctcc gaccatgaag ttcgcgatct
     3481 gcaatgagat ctttcaggaa tggaacgaca tcgcccgcac attcaactac gtcaaggaaa
     3541 tcggctacga tgggatcgaa cttgccccgt tcacgttcag ccagtacgtg accgacatca
     3601 gcccggaaac acgcaaagag attgtgaatc gagcccgcga agtcgatctt gacgtcatcg
     3661 gcatccactg ggtgttcgtc ggtccggaag gcctgcacat caatcatccc gacaaggaac
     3721 accgcgaccg tgccgcccag tacctcattg accttgctca attctgcggc gatgtgggcg
     3781 gccgtgtgat gatcttcggg tcgcctaaac agcgcaatgt ccacgaaaag ctgacgtatg
     3841 atcaggcctt tgaatacacc gctgccgtct tcgaacaggc gctgcccgcg tgcgaaaaaa
     3901 acggcgtgac catctgcatg gaaccgctga cccatctcga gaccaacttc tgcac
//
LOCUS       DAOPBQ010000360         3948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18663_length_3948_cov_4.061649, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000360 DAOPBQ010000000
VERSION     DAOPBQ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18663_length_3948_cov_4.061649"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..556
                     /gene="acpS"
                     /locus_tag="PKW60_11145"
     CDS             164..556
                     /gene="acpS"
                     /locus_tag="PKW60_11145"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPK00032.1"
                     /translation="MIVGIGTDLVDVAEMARRIERGRVLQVFSEEERAYADSKPKRRM
                     ETLAARWAAKEAFAKALGTGIRAEWRLEDIEVVHGAGGKPELRLGPSVADVLPEGARV
                     HVSLSHTKTSALAVVIIEAGQADPRANS"
     gene            complement(551..1546)
                     /locus_tag="PKW60_11150"
     CDS             complement(551..1546)
                     /locus_tag="PKW60_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00033.1"
                     /translation="MSNLRWGILSTARIGKRVIRGIGLSKACCMQAVASREKARAIEW
                     AKEHGIPRAFGSYEELLTSGEVDAIYNPLPNSMHAEWTIKALEAGLPVLCEKPFAISA
                     GEARTVVKTAQKKRLPVAEAFMYRYHPVYDRVWELIDEGAIGELVTIRSIFSFPLNDP
                     DNIRMKPELAGGALMDVGCYCVNFSRMMARCEPACATAFVRLGAVDKTTVACLEFPNN
                     LLAYFECSFETHTRMRAVLEGTDGALILPSPWFPGETRAEIILRRGDEEKREFVAGAD
                     SYQLEVEDFINACLTREPTRWPAEDAIANMAAIDAIRRSAKERGPVSVAKVQAVS"
     gene            complement(1669..2445)
                     /locus_tag="PKW60_11155"
     CDS             complement(1669..2445)
                     /locus_tag="PKW60_11155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK00034.1"
                     /translation="MKKTVVMGAVIALGFVSMAALNGCQPSRMTPERAHAAREVELRL
                     EQAEREQMKEDQRAASTPALERAPLPEGYNGGKSPADATLTAPDNFKVQFICSNGNFV
                     VECHRDWAPIGADQFYNLVLQGFYDDARFFRVLPGFVVQFGIHGDPAIMAPWRDANIK
                     DEPVKQGNRRGTLTFAKSSMPNSRSTQLFINLGDNSAGLDPQGFSAFGEVIDGMDIVE
                     SISSKYGEKPNQMMIQEQGNAYLKPNFPEMDYIKEAYIAQ"
     gene            complement(2538..3608)
                     /gene="pheA"
                     /locus_tag="PKW60_11160"
     CDS             complement(2538..3608)
                     /gene="pheA"
                     /locus_tag="PKW60_11160"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPK00035.1"
                     /translation="MNLDDLRKRIDALDSEILRCLNERARCAMEIGDIKRASNAVFYV
                     PERERAVYDKLVRENQGPLTEAGIKAIYREVISAVRALEKPVDVAFLGPRDTFSHKAA
                     LLVFGMHASYHPVAAVDDIFTEVERFRVDYGVVPVETSMGGGVSDTLDRFLTSDVKIV
                     NEIMLRIVQNLLSNSSLDEITKVYSKSQPFIQCRNWLKANLPNAELVETSSTAEAARI
                     AADEPGAAAIASELAAQTYNLNILVRGIEDASANFTRFFVIGRQLAKPTGNDKTSILC
                     YIKDRPGALYELLTPLREGKINLTRIESRPSRRKAWDYVFFIDLIGHCEDPAVASALQ
                     QLSHYCTELKVLGSFPGGDLQY"
     gene            complement(3605..>3948)
                     /locus_tag="PKW60_11165"
     CDS             complement(3605..>3948)
                     /locus_tag="PKW60_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00036.1"
                     /translation="LSLSRSRIVFGSRDWREASRFLGEVPAALLERLVPASSKAQQGK
                     SAPPAAPAAAPERLTLGTHVRHAQFGPGIIMYTKGTGSKMRARIRFHTGRTREFMVSV
                     APLEILERKKP"
BASE COUNT          710 a         1205 c         1250 g          783 t
ORIGIN      
        1 cgctggcccg gcaggtctcg tctggaccct ttcccgcggc ggggtggtgt atgatgccgc
       61 aggtaacggc ggatgcggcc ggcccccggg aacgccctca ggcagggagg ggcgccgcgc
      121 agccggggta tgttccacac attcaagagg agaagtcgca tcaatgatcg tcggtatcgg
      181 gaccgatctg gtggatgtag ccgagatggc ccgccgcatc gaacgcggcc gggtgctgca
      241 ggtgttcagt gaagaggagc gagcgtacgc cgatagcaag ccgaaacggc gcatggagac
      301 tcttgccgcg cgctgggccg cgaaggaagc cttcgccaaa gctctcggaa ccggcatccg
      361 cgcggagtgg cgcctggaag acatcgaggt ggtccatggc gcagggggca aacctgaact
      421 gcggctgggc ccgtccgtag cggatgtcct gcccgaaggg gcgcgcgtgc acgtgtcgct
      481 gtcccatacg aagaccagcg ccctggccgt ggtgattatt gaggccggtc aggccgatcc
      541 gcgcgcgaat tcatgaaacc gcctggacct tggcgacgct gaccgggccg cgctcttttg
      601 ccgaacggcg gatcgcgtcg atcgccgcca tgttggcgat ggcgtcctcg gcaggccagc
      661 gcgttggctc gcgagtgagg caggcattga taaaatcctc gacttcaagc tgataggagt
      721 ccgcgcccgc tacgaactca cgtttttcct catcgccacg ccgcaggatg atctccgccc
      781 gggtttcgcc ggggaaccac ggactgggca ggataagcgc gccgtcggtg ccttccagca
      841 cggcccgcat gcgggtgtgc gtctcgaaac tgcattcgaa atacgccagg aggttgttgg
      901 gaaactcgag gcaggccacg gtcgtcttgt ccacggcgcc caggcggacg aaagcggtcg
      961 cgcaggccgg ttcgcagcgg gccatcatgc gtgagaagtt cacgcaatag cagcccacat
     1021 ccatgagcgc gccgccggcc agctctggtt tcatgcgaat gttgtccggg tcgttcaggg
     1081 gaaaagaaaa gatgctgcgg atggtgacga gttcgccgat cgctccctca tcgatgagct
     1141 cccacacgcg gtcgtagacc ggatggtaac ggtacatgaa ggcctcggcg acggggagtc
     1201 tcttcttttg tgcggttttg acgaccgtgc gggcttcgcc ggcactgatc gcgaacggtt
     1261 tctcgcacag gacaggcagc ccggcttcca aagccttgat ggtccattcg gcgtgcatgc
     1321 tgttgggcaa ggggttgtag atggcgtcga cttcgcccga ggtcagcagt tcctcgtacg
     1381 aaccgaaagc ccgcgggatc ccgtgttctt tggcccattc gatggcgcga gccttttccc
     1441 ggctcgccac ggcctgcatg cagcacgcct tactcaagcc aatgcctctg atcacgcgct
     1501 tgccgatgcg agcggtgctc aggatgcccc agcgcaggtt tgacatgacc cacctcccgc
     1561 agaccgccgc ctgccccggg gacagaagcg gacgggctca tgaaccgcgg ccatggtaga
     1621 gcacaaaacg gggcggaacc cgaactcagg attccgcccc caatccagct attgggctat
     1681 gtacgcctcc ttaatgtagt ccatttcagg aaagttgggc ttcagatagg cattgccttg
     1741 ttcctggatc atcatctggt tgggtttctc accatatttg gatgagatgc tttcgacaat
     1801 atccatgccg tcgatcacct cgccaaacgc cgaaaagccc tgcggatcaa ggccggcgct
     1861 gttgtcgccc agattgatga acagttgcgt ggaacgtgag ttcggcatgg acgatttggc
     1921 aaacgtgagg gtgccccgcc ggttgccctg tttgaccggt tcgtccttga tattggcatc
     1981 gcgccacggg gccatgatgg cgggatcgcc gtgaatgcca aactgcacca caaagcccgg
     2041 cagaacccgg aaaaagcgcg cgtcgtcata aaacccctgc agcacaaggt tgtagaactg
     2101 gtccgcgccg atgggcgccc agtcccggtg gcattccacc acgaaatttc cgttgctgca
     2161 gataaactgg accttgaagt tgtccggggc ggtcagggtg gcgtctgcgg gcgatttacc
     2221 gccgttatag ccctcgggca ggggggcgcg ttccagcgcg ggggtacttg cggctctctg
     2281 gtcttctttc atttgttccc tttcggcttg ttccagtctg agttcaactt ctcgtgcggc
     2341 atgggcccgt tcgggggtca tccggctggg ctggcagccg ttgagggccg ccatactgac
     2401 gaaaccgagc gctatgaccg cgcccatgac tactgtcttc ttcacgctgc tgcctccctt
     2461 ttcttttctt tcttgccgtg ccccgggatc tcctgcccgt ggggagcggt ctcctccggg
     2521 aacgccggcg ttgccgtcta atactgaaga tcaccgcccg gaaacgagcc caggactttc
     2581 agttccgtgc agtagtgcga caattgctgc agcgccgatg ccaccgcggg gtcttcacaa
     2641 tggccgatga ggtcaatgaa gaagacgtaa tcccatgcct tgcgccgcga cgggcgggat
     2701 tcaatgcggg tcaggttaat tttaccttcc cgcagcggcg tcagcaactc atataaggcc
     2761 cccgggcgat ccttgatgta gcagaggatc gaggtcttgt cgttgccggt gggtttggcc
     2821 aattgccgcc cgatcacgaa gaaccgggtg aaattcgccg aagcgtcttc gataccacgc
     2881 accagaatgt tgaggttgta ggtttgcgcg gccagttcgc tggcaatggc ggcggcgccg
     2941 ggctcatcgg cggcgatccg ggcggcttcg gccgtgctcg aggtctcgac cagctcggcg
     3001 ttgggcagat ttgccttgag ccagttgcgg cactggatga acggctgcga tttcgaatag
     3061 accttcgtaa tctcgtccag actcgagttc gacagcagat tctgaacgat gcgcagcatg
     3121 atttcgttca cgatcttgac atccgaggtc aaaaaccggt cgagcgtatc gctgacgccg
     3181 ccgcccatcg acgtctcgac cggcactacc ccgtaatcga cccggaaccg ctcaacttcc
     3241 gtgaagatgt catcgactgc ggcgacgggg tggtaggagg cgtgcatgcc gaagaccagc
     3301 agcgccgcct tatggctgaa ggtgtcgcgc gggcccagga aagccacgtc cacgggcttc
     3361 tccagagcgc ggacggcgct gatgacctcg cggtaaatgg ctttaatccc ggcctcggta
     3421 agcggtccct ggttttcacg cacgagcttg tcatagacgg cgcgctcgcg ctcgggtaca
     3481 taaaatacgg cgttactggc ccgtttgatg tcgccgattt ccatcgcaca ccgggcccgc
     3541 tcgttgaggc agcggaggat ttccgaatcc agcgcgtcga tccgttttcg caggtcgtcc
     3601 aggttcacgg ttttttcctt tcgagaatct ccagcggcgc aacactgacc atgaactcgc
     3661 gggtgcgccc ggtgtgaaac cgtatccggg cacgcatttt ggagcccgtc cccttagtgt
     3721 acatgattat gcccgggccg aattgggcgt gccgcacatg cgtgcccagc gtgagccgct
     3781 ccggggcggc tgctggcgcg gccgggggag ccgatttccc ctgctgcgcc ttcgagcttg
     3841 ccggcaccag ccgttcgagc agcgccgccg gaacctcgcc cagaaaccgc gatgcctcgc
     3901 gccagtcccg cgacccaaac acgatccgcg agcgcgacag cgacagcg
//
LOCUS       DAOPBQ010000361         3937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18745_length_3937_cov_3.993045, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000361 DAOPBQ010000000
VERSION     DAOPBQ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18745_length_3937_cov_3.993045"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PKW60_11170"
     CDS             <1..871
                     /locus_tag="PKW60_11170"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPK00037.1"
                     /translation="TLFHRHDRRSYLSPLMSTPEDVRVLVDESAQHGTIDAEEQRMIH
                     AIFDLQNMLAKEIMVPRIDISALPDTTTRADLLATFEASGKTRIPIYRESIDQIVGAV
                     TAHDILLDTRSGSEGIRPLIRDIMHVPDTITLGDVFEEMKRRKQHIAIVIDEYGGTDG
                     LITLEDILEVIFGQIQDEHDRELRSIQRVGPDAYVIDGRMNLEEAAEFMEVVIEDDEV
                     ETVGGWLMHVAGRIPAQGEVIMHNGFRVIVLEGGLNHITKLRLEILPEARETYGIQAH
                     ETKANRRHGPTVS"
     gene            828..1625
                     /locus_tag="PKW60_11175"
     CDS             828..1625
                     /locus_tag="PKW60_11175"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HPK00038.1"
                     /translation="MKQKRIDAMARPSPDRSLWGGIRFLYGKLWRILVAGFLLWLPLI
                     ITLWVSWWVFSKLVFGIERLIKAIVLNLHVLAERVPALGFFSLVRYQPGLGILLTITI
                     FLVSGYLARYLIGRRLIAFGERLFRFIPFFNRIYQAVVQIRDVFVSRQGTVFQRACLV
                     DYPRAGMTAVAFVTSSEQGSIQRRKGKELIAVFVPTTPNPTSGYLIYIPPEDITEIDM
                     PVEEAMKLIVSGGAYLPSRHGWLPGDELPDGTDSPKSDTAPDQETAD"
     gene            complement(1710..2615)
                     /locus_tag="PKW60_11180"
     CDS             complement(1710..2615)
                     /locus_tag="PKW60_11180"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPK00039.1"
                     /translation="MEFDPAVKLTRENIKDLPPLCTDGAWGTEMAKQGSGDPGYVCDC
                     WNLERPEVVLAVARAYVDAGAQIILTNTFNSNPIALHRHNLQNKAAELNRTGAEISKR
                     AAAGKAYVFASMGPCGKMVMMGDIDPAVVEEAAARQAHVLESGGADAIVIETQSDIAE
                     AEALLKGVLRGCSLPVGVSFTFDSGENNDKTMMGVSIPQAYAMARTFGASFVGANCGA
                     GIDTFPNVAEQFAACGSDLPIWIKGNAGLPETDGLGNMIFRAGPDIFAQTVPLLLKAG
                     ARFIGGCCGSTPDHVRAIAQAMAAA"
     gene            complement(2740..3786)
                     /locus_tag="PKW60_11185"
     CDS             complement(2740..3786)
                     /locus_tag="PKW60_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPK00040.1"
                     /translation="MVGGSPRILIIRLSAIGDVVRVLPALQILRDALPDAHIDWVVEK
                     KSADICRDHPSLDGILVFERPEGARNAVKSFLALCSDIRNRRYDIVIDFHGVLKSGLL
                     AWASRAPKRYGFARPRSQECSFLFTNRRTRLPARTLNRVEENMILCEAVVPVRAGCPS
                     PTIEIPPEVQEAVDDYIDRTFEAGKRLVAVHAPVDRREKRWPLDYFAEVCDLLIADGR
                     FEVMLTWGPGQQEIAQKVFARCRRASVIAPETPDLKHYAALLSRTHLYVGGDTGPMHI
                     AAAMGTPVVAIFTGTNAVQHAPYRSPCEVLTPERAHELLPQGESTGETLSPITPEMVY
                     DACVRLLSPKQTAR"
BASE COUNT          724 a         1267 c         1215 g          731 t
ORIGIN      
        1 gacgctgttt caccgccacg accggcgcag ctacctcagc ccgcttatgt ccacacccga
       61 agacgtgcgc gtcctcgtgg acgaaagcgc ccagcacggc actatcgacg cggaagaaca
      121 gcgcatgatc cacgccatct ttgacctcca gaacatgctg gccaaagaga tcatggtgcc
      181 gcgcatcgac atcagcgccc tgcccgacac cacgacccgc gcagatctgc tcgccacgtt
      241 cgaagcatcg ggcaaaacgc gcatccccat ttaccgcgaa tcaatcgacc agatcgtcgg
      301 cgcggtcacg gcacacgaca ttctgctcga tacccggtcc ggcagcgagg gcattcgccc
      361 tctcatccgc gacatcatgc acgtgccgga tacgatcacc cttggcgatg tgttcgagga
      421 aatgaaacgc aggaagcagc acattgccat tgtaatcgac gaatacggcg gaaccgacgg
      481 gctcatcacc ctggaagaca tcctggaggt gattttcgga cagatccagg acgaacacga
      541 ccgcgagctg cggtccattc agcgcgtcgg ccccgacgcc tatgtcatcg acggccgcat
      601 gaatctcgaa gaagccgccg agttcatgga agtggtcatc gaagacgacg aagtcgagac
      661 cgtcggcggc tggctgatgc atgtggcagg ccgcatcccc gcacagggcg aagtgatcat
      721 gcacaacggg ttccgggtca tcgtactgga aggcggcctc aaccacatca ccaaacttcg
      781 cctcgaaatc ctccccgaag caagagaaac ttacggcata caagcacatg aaacaaaagc
      841 gaatagacgc catggcccga ccgtctcctg atcgaagcct ctggggagga atccgcttcc
      901 tctacggcaa actctggcgc atcctggtcg cgggattttt gctgtggctc cccctgatca
      961 tcaccctgtg ggtatcctgg tgggtgttca gcaagctcgt cttcgggatt gagcgcctca
     1021 tcaaggccat tgtgctcaac ctgcacgtcc ttgctgaacg cgtgcccgca ctggggttct
     1081 tctccctcgt gcgataccag cccggcctgg gcatcctgct cacgatcacg atcttcctgg
     1141 tcagcggcta cctcgcgcgc tacctcatcg gacgccggct catcgccttc ggcgagcgcc
     1201 tcttccggtt catccccttc ttcaaccgca tctaccaggc ggtcgttcag atccgcgacg
     1261 tcttcgtcag ccgccagggt accgtgttcc agcgcgcctg cctcgtcgat tacccccgcg
     1321 cgggcatgac cgccgtcgcc ttcgtcactt cgtccgagca gggatccatc cagcgcagaa
     1381 aaggaaaaga actcatcgcc gtgttcgtcc ccaccacccc caacccaacg tcgggctatc
     1441 tcatttacat ccccccggaa gacatcaccg agatcgacat gcccgtcgaa gaagccatga
     1501 aactcatcgt ctccggcggc gcctacctgc cctccaggca tggctggctg cccggcgacg
     1561 aactccctga tggcaccgat tcccccaagt ccgacacagc ccccgaccag gaaacggcgg
     1621 actgaaaacc tctccaggct gtgatgcagg gcgcacagcg cgggtggaac ctgccgggcg
     1681 tccggcaggc tggaggctca ggcggaggct caggcggcgg ccatggcctg agcgatggcg
     1741 cgcacgtggt caggagtcga accgcagcag ccgccgatga accgcgcgcc cgccttgagg
     1801 agcagaggga ccgtctgcgc aaagatgtcg gggccggcgc ggaagatcat gttgccgagg
     1861 ccgtcggtct cgggcaggcc ggcgttgcct ttaatccaga tggggagatc gcttccacag
     1921 gcggcgaact gttccgcgac attggggaag gtgtcgatgc cggcgccgca attggcgccg
     1981 acgaaactgg cgccgaaggt cctggccatg gcataggcct gcgggatgct cacgcccatc
     2041 atggttttgt cgttgttttc gcccgagtcg aaggtgaacg acacgccaac gggcaggctg
     2101 catccgcgca acacgccttt gagcaaggcc tcggcttcgg cgatgtcgct ctgggtttcg
     2161 atgacgatgg cgtcggcgcc gccggattcc aggacgtgcg cctgccgtgc cgcggcctct
     2221 tccacgacgg cggggtcgat gtcgcccatc atgaccatct tgccgcacgg gcccatggac
     2281 gcgaacacgt aggctttgcc ggcggcggcg cgcttggaga tttcagcgcc cgtgcggtta
     2341 agttcggcgg ctttattctg aaggttgtgg cggtgcaggg cgatggggtt gctgttgaag
     2401 gtgttggtga ggatgatctg ggcgccggcg tcgacgtagg ccctggcgac cgccagcacg
     2461 acctcgggcc gttcaaggtt ccagcagtcg cagacataac ccggatcgcc cgagccttgt
     2521 ttggccattt cggtgcccca ggcgccgtcg gtacacagtg gcggtaaatc cttgatgttt
     2581 tcgcgggtga gtttcacggc gggatcgaat tccatggcag acctcgcttc ctatcgtttc
     2641 actcggctac ctgcgcacgt gcgttccgga atcgcccgga ccggattcat tttcgcagac
     2701 tcgcggccga attgccagcc tttttggtgc gggagcgcgt tatctggccg tctgcttggg
     2761 ggagagcagc cgaacgcatg cgtcgtagac catctccgga gtgatggggc tgagggtttc
     2821 tccggtgctt tcgccttgag gaagcagctc gtgggcgcgt tcgggcgtga gcacttcgca
     2881 cggactccgg tacggcgcgt gctgtaccgc gttggtgccg gtgaaaatgg ccaccaccgg
     2941 cgtgcccatg gccgcggcaa tgtgcatcgg acccgtatcg ccgccgacgt agaggtgggt
     3001 gcggctcagc agcgcggcgt aatgtttgag gtcgggtgtt tccggcgcga tgaccgaagc
     3061 gcggcggcag cgcgcaaaaa ccttctgtgc gatctcttgc tgacccggcc cccaggtcaa
     3121 catcacttcg aagcggccat cagcgatgag caggtcgcac acttcggcaa aatagtccag
     3181 cggccagcgt ttttcacggc gatcgaccgg cgcatgcacc gccaccaggc gtttcccggc
     3241 ctcgaaggtg cggtcgatgt agtcatccac cgcttcctgc acctcgggcg ggatttcaat
     3301 ggtcgggctg ggacaccccg cccggaccgg cacaaccgcc tcgcacagga tcatgttctc
     3361 ctcgacccgg ttcaaggtgc gtgcaggcag gcgcgtgcgc cggttcgtga acaaaaaact
     3421 gcattcctgg ctccggggcc gggcaaatcc gtagcgtttc ggggcgcggg acgcccacgc
     3481 gagcaggccg ctcttcagaa caccgtggaa atcgatcacg atgtcgtacc ggcggtttcg
     3541 gatgtcgctg cacagtgcca gaaacgattt cacggcgttc cgggcgcctt cgggccgttc
     3601 aaacacgagg atgccgtcca gggacgggtg atcgcggcag atgtcggccg atttcttttc
     3661 gacgacccag tcgatgtgcg catcgggcag ggcgtcgcgg aggatctgga gcgccggtaa
     3721 aacccgcacc acatcgccga tggcgctcaa gcggatgatc aggatacgag ggctgccccc
     3781 caccatattg ggtgcgcgtt ccttattctg gcctggagcc cgggatgcgc tccggcagtc
     3841 gccgggcatc ctccgctccc gtggttactc cccgattggg gatatggcgg agagggaggg
     3901 attcgaaccc tcggtacgcc ttgcgagcat acacacg
//
LOCUS       DAOPBQ010000362         3937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18746_length_3937_cov_3.161257, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000362 DAOPBQ010000000
VERSION     DAOPBQ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18746_length_3937_cov_3.161257"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PKW60_11190"
     CDS             <1..858
                     /locus_tag="PKW60_11190"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPK00041.1"
                     /translation="GYGHGFSKHRAVSAVPTRQGPPVLQYMTYYSQQYEYRMAPRSNR
                     ITLGVQRILLLSIAVFAVQLVLHMVEPLATRKVAPMNGEPGLEMLRWFVFEPARFPWN
                     GFIWQPLTYMFLHAGLMHLFTNMLWLFVFGPDVERVLGTRQFIRFYLSVGALSVLPTL
                     VTARFWGGAPVIGASGATMGVLVAFAVVNPDRELFLFPIPIRLTARALVIIVIALNLM
                     SAIRPDGTSVETHFGGLAAGYAYMKLVPWWRSLRRRHKDKVSEDPLDAIGEAVNNIFS
                     YEKEKRRRK"
     gene            880..1248
                     /locus_tag="PKW60_11195"
     CDS             880..1248
                     /locus_tag="PKW60_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPK00042.1"
                     /translation="MFGFASPQEMLFVVLIIVVMWITGLWPIVIRGIRELRGERFDDP
                     PASKAHQSRHQTKAPASPRDLDLCYKMLGISPSAPWEEVEKAYRRKAKIHHPDHGGDE
                     DAMRALNEAYSQLKIIRNRR"
     gene            complement(1261..1677)
                     /locus_tag="PKW60_11200"
     CDS             complement(1261..1677)
                     /locus_tag="PKW60_11200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPK00043.1"
                     /translation="MNDTPLVTFETTGSITVGRIGAASVLDAVNVTQFGNEVLEFVKN
                     RPGINLMLDFVHVDYLSSAVLTELLRINQVVQKEQGSLRLCALKPDVLKVFEITNLDK
                     VFTIYGESAQALKRFERSLEVAEREDLWSRLAKEED"
     gene            complement(1674..2111)
                     /locus_tag="PKW60_11205"
     CDS             complement(1674..2111)
                     /locus_tag="PKW60_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK00044.1"
                     /translation="MIENKAFYRAEFASTLDALGDAIAEALETLRAHDWIDEDQVFYA
                     RLCLEEALVNAITHGNQLDDTRKVRIEMTRETPGDICCIRVCDEGPGFEPDAVSLPSG
                     ASPNGRGVCLIRHCMESVTYNPEDRCLEMRMRRKGLCRGGAVS"
     gene            2408..>3937
                     /locus_tag="PKW60_11210"
     CDS             2408..>3937
                     /locus_tag="PKW60_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00045.1"
                     /translation="MPRFLLVSLTALLVMLPGSGASGAQNTETRPETTAEAMPLRLGG
                     AGGLYLFASPGPLVVEIAKQDINNRAIETHLRAILFDAGRDVIQEAYLPDDGQAAGSG
                     PGPVQWCRLETVVEAAGVYGVNITVTNDRYGEHAYWGFTTNCPQYLVETSRGHRDAPH
                     EEPIVIDSPEREGTLCFLPRAEAFAIEMTGLPPESAPVKLYDAADALLAEIPVMEDGR
                     ASHEVPAAAREHVPWRLHFAKYKAVVQIDGVTRWDSRSNNLSLWTPEPASWFPFHANR
                     WLLSPYHRVVYAQPGESRSLTFRIHNNGSEPRSVALGLETEPAAAPVFSLSELPDARV
                     SLKPGEAREVSLACHLPEDGGMYRCRLRATPEDGSGFTTYSTVTLRPGEAPAAAPLTL
                     PITLKPYQHENAQFGYLPDYPTANQVYFDPENRPCVITDTALWTVRDQSWQALPLKTA
                     EGASIVARTTKVAFDSAGNAYFIGTMGGKAVYARLPAAGTAFEAYPIPGGGSFDIEQY
                     SG"
BASE COUNT          725 a         1244 c         1182 g          786 t
ORIGIN      
        1 ggctatgggc atggattctc gaaacacagg gccgtatccg ccgtgccgac ccgccaggga
       61 ccgccggttc tccagtatat gacatactat tcccaacagt acgaatatcg catggctccg
      121 cgcagcaacc gcatcacact gggtgtgcag cggatcctgt tgctgagtat tgccgtattt
      181 gcggttcagc tggtgctgca catggtcgag ccgctggcga cccggaaggt ggcgccgatg
      241 aatggcgagc cggggctcga gatgctccgg tggtttgtat tcgagcccgc ccgttttccg
      301 tggaatgggt tcatctggca gccgttgacc tacatgttcc tgcacgcggg cctgatgcat
      361 ctcttcacaa acatgctgtg gctgttcgtg ttcggccccg atgtggagcg ggtgctgggc
      421 acacggcagt ttatacgctt ttacctgagc gtcggagctt tatcggtact gcccacgctg
      481 gttacggcgc ggttctgggg cggcgcgccg gtgatcgggg cgagcggcgc caccatgggc
      541 gtgctggtgg cgttcgccgt ggtgaatccc gaccgggaac tcttcctgtt ccccatcccg
      601 atccggttga ccgcgcgggc cctggtgatc atcgtcatcg ccttgaacct catgtcggcg
      661 atccggccgg acggaacctc tgtggaaaca cacttcggcg ggctcgcggc gggctacgcc
      721 tatatgaagc tggtgccgtg gtggcgctca ctgcgccgcc ggcacaaaga caaggtgtcc
      781 gaagatccgc tggacgccat cggcgaggct gtgaataata tcttcagtta cgagaaggaa
      841 aagcggcgca gaaaatagct gcgcaaggag cttgcggccg tgttcgggtt tgccagtccc
      901 caggagatgt tgttcgtcgt cctgataatc gtggtgatgt ggatcaccgg gctgtggccg
      961 atcgtcatcc gtgggatccg ggagttgcgg ggcgaacgct tcgacgaccc gcccgcgtca
     1021 aaggcacacc agtcacgcca tcagactaag gcgccggcct caccgcgcga tctggacctc
     1081 tgctacaaaa tgctggggat ttcgccgtct gccccatggg aagaagtcga gaaggcctac
     1141 cgccgaaaag ccaagattca ccatcccgac cacggcggag acgaagacgc catgcgcgcc
     1201 ctcaatgaag cgtactcgca gctcaaaatc attagaaacc ggcgctgaac ctcgtgccgg
     1261 tcagtcttct tctttggcga ggcgcgacca caaatcttcc cgttccgcga cctccagcga
     1321 ccgttcgaat cgcttcagcg cctgggcact ttcgccgtaa atggtgaaca ctttgtcgag
     1381 gttcgtgatt tcaaagacct tgagcacatc cggtttgagc gcacacaggc gcaggctgcc
     1441 ctgctctttc tgaaccacct gattgatgcg gagcaattcc gtcaacacgg cgctggacag
     1501 gtagtccacg tgcacgaagt ccagcatgag gttgataccc ggcctgtttt tcacaaattc
     1561 gaggacctcg ttaccgaatt gcgtgacatt cacggcatcg agtacgctcg cggcgcctat
     1621 gcgtccaact gtgatactac cggtcgtctc aaaggtcacc agcggtgtgt cgttcatgat
     1681 actgcgcctc ccctgcacaa acctttacgc cgcatgcgca tttccaggca acggtcctct
     1741 ggattgtatg tgacactctc catgcaatgc cgaatgaggc acactccccg gccgttcggc
     1801 gatgcacctg agggcaggct gaccgcatcg ggctcgaatc cggggccttc atcgcagacg
     1861 cgaatgcagc agatatcacc gggcgtttcc cgagtcatct caatccgcac ctttcgcgtg
     1921 tcatccaact ggttgccgtg cgtgatggcg ttgaccagag cctcctccag gcacaaccgg
     1981 gcataaaaaa cctggtcttc gtcgatccag tcatgcgcac gcaaggtctc cagagcctcg
     2041 gctatcgcgt cgccgagcgc atcaagtgtg cttgcgaact ctgcgcggta gaaggccttg
     2101 ttctcaatca cgccggctta tccacctctg cactgcgaat actgcgccct ctgtgccatc
     2161 caatccagca taccacaggg agccgtacga ggtgtagaac cccgaaacgg cccgcagtaa
     2221 tcctgaccgg ttccgcctct gcccatcccg cggatgtatc cggtgcggag tcgccgctgg
     2281 cttatggccc cggctggctg ccggttcgcc cgccttgcgc attccaacac ctgaacatgc
     2341 tacagtgtcc gtcgtatttt gaacatgccc gcgagatctg gagctttcac ccgaaaggac
     2401 agctgccgtg ccacgttttc tcttggtcag cctgaccgct cttcttgtga tgttacccgg
     2461 gagcggtgcc tccggggccc aaaacaccga gacccgtccg gagaccaccg cagaagcgat
     2521 gcccctgcgc ctgggcggag ccggaggcct gtatctattc gcctcacccg ggccgctcgt
     2581 ggtggagatc gccaaacagg acataaacaa ccgggcgatc gagacgcacc tccgcgccat
     2641 cctgttcgat gccgggcgcg atgtcatcca ggaggcatat cttccggacg acgggcaggc
     2701 ggccggaagt ggccccgggc cggtgcagtg gtgccggctg gagactgtcg tggaggccgc
     2761 aggcgtctac ggcgtcaata tcaccgtgac caatgaccgg tatggcgagc atgcgtactg
     2821 gggattcacc acgaattgcc cacaatacct cgtggagact tctcgagggc accgcgacgc
     2881 tcctcatgag gagcccatcg ttattgacag cccggagcgc gagggcacgc tgtgttttct
     2941 gccgcgcgcg gaggcctttg ccattgaaat gaccggcctg cctccggaga gcgcgccggt
     3001 aaagctgtat gacgccgcag atgcgctcct ggccgagatc ccggtgatgg aggatggccg
     3061 ggcaagccac gaggtgccgg cggctgcccg agaacatgtc ccctggcggc tgcatttcgc
     3121 gaaatacaag gcggtggttc agatcgacgg cgttacgcgc tgggactcac gcagcaacaa
     3181 cctgtcgctc tggacgcctg agcccgcgtc gtggtttccg tttcacgcga accgctggct
     3241 gctgagcccc tatcaccgcg tcgtgtatgc ccagcccggc gaatcgcgct ccctgacgtt
     3301 tcgcattcac aacaatggct cggaaccgcg cagcgtcgcc ctcggcctgg agaccgagcc
     3361 ggccgccgct ccggtattca gcctttcgga gctgccggat gcccgcgtga gtctgaagcc
     3421 gggagaagcg cgcgaggtgt ccctcgcatg ccatctgccg gaggacgggg gaatgtatcg
     3481 atgccgcctc cgcgcgactc cggaagacgg ctccggtttt accacgtatt cgactgtgac
     3541 gctgcggccc ggcgaagcgc ctgccgctgc acccctcacg ctgcccatta cgctgaaacc
     3601 ctaccagcat gagaacgccc agttcggcta cttgccggac tatcccaccg ccaaccaggt
     3661 ctatttcgac cccgaaaacc gcccctgtgt catcactgac accgcgttgt ggacggtccg
     3721 ggatcagtcc tggcaggccc tcccgctgaa gacggcggaa ggcgcttcta ttgtggcgcg
     3781 tacgacgaaa gtggccttcg attcggccgg aaatgcctat tttattggca cgatgggtgg
     3841 aaaagcggtt tatgcgcggc tgccggccgc aggtacggcc tttgaggcgt atccgatccc
     3901 gggaggcggg tctttcgaca tcgagcagta ctccggc
//
LOCUS       DAOPBQ010000363         3917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18858_length_3917_cov_3.381409, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000363 DAOPBQ010000000
VERSION     DAOPBQ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18858_length_3917_cov_3.381409"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKW60_11215"
     CDS             <1..794
                     /locus_tag="PKW60_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPK00046.1"
                     /translation="GQRVAVEPGIPCLKCEWCNTGHYNVCPDMVFPGGPPYDGALTEY
                     IAVHARFCFPVPDTINAAEAAMIEPLAVAVHAVELACLKPGETVAVLGLGPIGLLVAQ
                     VARAAGAAQIYGTDLLDYRVSAGERFGVDVAFNASRADTVDTILSKTSGRGVDVAIDC
                     ARSSETQGHACRVARPAGRVVLAGISGEEEDPFPVCVARRKELTLQWCRRFRFDYPRS
                     IALLAAGRVDVASLVTHSFPLERSAEAFELVMRFDDNVLKASIDQ"
     gene            840..2240
                     /locus_tag="PKW60_11220"
     CDS             840..2240
                     /locus_tag="PKW60_11220"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HPK00047.1"
                     /translation="MKKRLLLTGAVLVVLLAGVLLLVGPWPAYSSADITQERYYQRNV
                     DAIQASAARSTIGGPPGPLRAGWAQRSIVPPPGTPLAGFGDRKGKPSTGVHDEIFVKA
                     LVLADGNDKTALIAADMLIIPENLAEIVRERVLRQTGLTADVLLFNATHTHSGPGGWA
                     PGFVGKQFAGTYDAAVLECLAQQFTDAIVDANANVEGGAAIGAGSVPVDACIRNRTRE
                     APCDTELSYLLVEKDDGARCLVVSYSAHATVLGGSNMEFSGDYPGYLVRALERETAAF
                     TMFLGGAVGSMSARHSGPEAGFEKARAIGEELARHVLERVDGLELRRDVEIASVGVPL
                     ELPSTQWRIHPSWRLSPFFVRFLGINSDGWLQCVRVGDVYFAGMPCDFSGEISLRLKA
                     WADTQGVDLWVLSFNGDYVGYVSPDEYYAAARPGGKEAYEMYLMNWCGPNQEAFFTHL
                     TREAVAALSRSAASGS"
     gene            2248..3621
                     /locus_tag="PKW60_11225"
     CDS             2248..3621
                     /locus_tag="PKW60_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00048.1"
                     /translation="MATITQSQGLIKPFQTGAILLLCGLVAIFTAASRERSLESGVPW
                     RDLAIAVEAAYVPEGALPLRTRLIPESNLPEATQELLERMRAKSGSEEALQLLGLSPT
                     ERLLMVDAPPDFFAPALLSGRLPQAGRPEVLAGALVRFDSFEMDGTTFQVVGRLSPQT
                     PGTLEACFLPADPAFDALFSDPELSTRGWLDPDALARLDALKEAVTSGEDAPRFMMPA
                     GRLPASITALTLSGLVLVAAGGALLQMRLLQSLAPHGGTLLGPCLTELNNRRMLLAAV
                     HVLLYATLFGAMAGGILLPRASWNALDFTRSVFSEGVLGHIGRAYASGNILWATLATL
                     HQNYLVGTCLYSVLPSLVFPFAGLLKNLLSFGFVGFVMAPMWTGSAVQNTYHSITLTL
                     ELEAYVVVSFAVCVLPLRVLRGIQTGDWPAEYVAGLRALVGATALAGIMLTIAAFYEA
                     ATLILLH"
     gene            complement(3658..>3917)
                     /locus_tag="PKW60_11230"
     CDS             complement(3658..>3917)
                     /locus_tag="PKW60_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00049.1"
                     /translation="QFRDKLVRETNVFFEVFESDAPAFLFVMENDWRWILAIHPSACA
                     YPIIRSEQVGSRYVLLIANKAGAASLEKHGIDIQIKRPGAA"
BASE COUNT          644 a         1133 c         1352 g          788 t
ORIGIN      
        1 tgggccagcg cgtggccgtg gagccgggga tcccctgcct taagtgcgaa tggtgcaaca
       61 ccggccacta caatgtgtgc ccggatatgg tgttccccgg cggtccgccg tacgacggcg
      121 cgctgactga atatattgcc gtgcatgccc ggttctgttt tcccgtgccg gacaccatca
      181 acgcggcgga ggcggcgatg atcgagcctc tggctgtggc tgtgcacgcg gtggaactgg
      241 cctgtctgaa accgggggaa acggtggcgg ttctgggact gggcccgatc ggcctgctgg
      301 tagcacaggt cgcccgggcg gcgggcgcgg cacagattta cgggactgat ctgctcgatt
      361 accgtgtgtc ggccggcgaa cggttcggag tggacgtggc cttcaatgcg tcgcgtgccg
      421 acacggtgga caccatttta tcgaaaacga gcggacgcgg cgtcgacgtg gctattgact
      481 gcgcgcggtc ctctgagacc caggggcatg cctgccgcgt tgcgcgtccg gcagggcggg
      541 tggtactggc cggcatttcc ggcgaagagg aagatccgtt cccggtatgt gttgcgcggc
      601 gcaaagagct gacgcttcaa tggtgcaggc ggtttcgctt cgactacccc cggtcgatcg
      661 ctctgctggc cgccggacgc gtggacgtgg cgtcgctcgt gacgcactcg tttccgttgg
      721 aacggtcggc ggaggcgttt gagctggtga tgcggttcga cgataacgta ttgaaggcat
      781 cgatcgatca gtagtcgcgc gcaaacccaa tcggaccgcc ggtacggaga ggcagatcgg
      841 tgaaaaagag actactcctt acaggcgcgg tacttgtggt gctgctggcg ggcgtgttgc
      901 ttctcgtggg gccgtggccc gcatattcca gcgcagacat cacgcaagag cgttattatc
      961 agcgcaatgt cgacgccatc caggccagtg cggcgcgttc cacgattggc ggtccgccgg
     1021 gccccctgcg tgcgggctgg gcgcaacgct cgatcgttcc gccgccgggt acgccgctgg
     1081 ccggatttgg cgacaggaaa ggcaagccgt ccacgggagt gcacgacgaa atcttcgtga
     1141 aagccctggt gctggccgat ggaaacgata aaaccgccct gatcgcggcg gatatgctca
     1201 tcattcccga aaacctggcg gagatcgtgc gcgagcgcgt gttgcgtcag actggcctca
     1261 cggccgatgt cctgctgttt aatgcgacgc acacacactc gggcccgggc ggctgggcgc
     1321 cgggttttgt gggaaaacag tttgcaggaa cctacgacgc cgccgtgctc gaatgcctgg
     1381 cgcagcaatt cacggacgcg atcgtggacg ccaacgcaaa cgtggaaggc ggcgcggcaa
     1441 ttggcgcggg gagcgtcccg gtcgatgcct gtatccgaaa ccggacccgg gaagcgccgt
     1501 gtgacaccga attgagctac ctgctggtcg agaaggatga cggggcccgc tgcctcgtgg
     1561 tgagttattc ggcgcacgcc acggttcttg gtggaagcaa tatggaattc agcggggact
     1621 atccgggcta tctcgtgcgg gcgctcgagc gcgagacggc cgcgttcacg atgtttctcg
     1681 ggggagcggt gggcagcatg tcggcgcgcc attccggccc cgaagccggc tttgaaaaag
     1741 cgagggctat cggcgaagag cttgcgcggc atgtgctgga gcgcgtggat ggcctggaac
     1801 tccggcgcga cgtggagatc gcctcggtgg gggtgccgct ggaattgccg tcgactcagt
     1861 ggcgcattca cccgtcgtgg aggttgtcgc ctttctttgt gcgattcctt gggatcaatt
     1921 cggatgggtg gctgcagtgc gtccgagtcg gcgacgtgta ttttgcgggc atgccatgcg
     1981 atttcagtgg cgagataagc ctgcggttga aagcctgggc cgacacgcag ggcgtcgacc
     2041 tgtgggtgct gagtttcaac ggcgactacg tgggatatgt gtcgccggac gaatattatg
     2101 cggccgcgcg gccaggcggc aaggaagcgt acgagatgta cctcatgaac tggtgcgggc
     2161 cgaaccagga ggcgtttttc acccacctca cccgggaagc cgtggcggcg ctttcgcgct
     2221 cggccgcgag cgggtcgtaa agcccgtatg gcgacgataa ctcaatccca gggcctcatc
     2281 aaacccttcc agacaggcgc tatcctcctg ttatgcggat tggtggcgat cttcaccgcg
     2341 gcaagcaggg aacgctccct ggaaagcggc gttccctggc gtgatctggc aattgccgtg
     2401 gaggccgcat atgtgccgga gggagcgttg ccgttgcgca cccggctgat tccagaaagc
     2461 aaccttcccg aggcaacaca ggaacttctg gaacgaatgc gggcgaaatc cggcagcgag
     2521 gaggctttac agctgctcgg attatcgcca acggagcggc tgctcatggt ggatgctccg
     2581 ccggatttct ttgcccccgc gctcttgtct ggacggctgc cgcaagcggg ccgcccagag
     2641 gtgctcgcgg gggcgttggt ccggttcgac tcgtttgaga tggacgggac aacgtttcag
     2701 gtcgtagggc gcctgtcccc acagactccc gggacactgg aggcctgctt tttgccggca
     2761 gacccggcgt ttgatgcgct gttctccgat ccagagctca gcacgcgggg ttggctggat
     2821 cccgacgcct tggcgcgtct cgacgcgctc aaagaggcgg tcacgtccgg tgaagacgcc
     2881 ccgcgtttca tgatgccggc cggccggctt cccgcttcga tcacggcgtt gacgctgagc
     2941 ggattggtgc tggtggccgc gggtggcgcg ctgctccaga tgcgcttgct gcaatccctc
     3001 gcaccccacg gcggaactct gctggggccc tgtctcacgg agctgaacaa ccggcggatg
     3061 cttctggccg ctgtgcacgt gctcctgtat gccacccttt ttggggcgat ggccgggggc
     3121 attctcttgc cccgcgcctc ctggaatgcg ttggacttta cccggtcagt cttttcggaa
     3181 ggggtgctgg gccatatcgg gcgggcgtat gcgtcgggaa acattctgtg ggcgacgctg
     3241 gcgacgctgc accagaacta cctcgtaggc acctgcctgt attccgtgct cccttcgctg
     3301 gtttttccgt ttgcgggatt gctcaaaaac ctcctgagct tcggttttgt gggatttgtg
     3361 atggcgccga tgtggacggg cagcgccgtg cagaacacgt accacagcat cacgttgacg
     3421 ctcgagcttg aagcctacgt ggtggtgtcg tttgcggtat gcgtgcttcc gctgcgcgtg
     3481 cttcgtggga tacagacggg ggactggccg gcagaatatg tggcggggct gcgtgctctt
     3541 gtgggcgcga ccgcgctggc agggatcatg ctcacgatcg ccgccttcta cgaagcggcc
     3601 accctcatcc tgctccattg agccgggcga gccgcgcctc cggcggccca aaatacttca
     3661 cgcggcgccg gggcgtttga tctggatgtc gatgccatgc ttttcgaggc tggccgcccc
     3721 ggctttgttc gcgatgagca gcacgtagcg actgccgacc tgttcgctgc ggatgatcgg
     3781 ataggcgcaa gcgctgggat ggatggccag gatccagcgc cagtcgtttt ccatgacgaa
     3841 cagaaaggcg ggggcatcgg attcgaagac ctcgaaaaag acgttggttt cgcggacaag
     3901 tttgtcccgg aactgtt
//
LOCUS       DAOPBQ010000364         3916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18865_length_3916_cov_3.312872, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000364 DAOPBQ010000000
VERSION     DAOPBQ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18865_length_3916_cov_3.312872"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            822..2402
                     /locus_tag="PKW60_11235"
     CDS             822..2402
                     /locus_tag="PKW60_11235"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HPK00050.1"
                     /translation="MMVPRIATDPKDKNLIFEAGERISDRYEIEEFLGRGGMGVVYRA
                     RDHLTNEQVALKFMIPDLLKSPKAQQLFIQEAQVARRLRHENIVAVHDVSTTPEGVLY
                     LSMEFLRGQSLRGLLRKYRKERRMLDVRVAVHATAQVLKALDYAHRMVIHRDIKPENA
                     MMLPGEHIKVLDFGLAKAVDTEAAAAGDTQRKRVVGTAAYASPEQLNLRPVDSRADLY
                     STGLLFYELLTLRAPTEEQIAVTEARSDIAPSLVAILNKALQEDPANRWQTAGEFRAQ
                     LTEAYQASYRHAARTRIPTVDQTREVSTEGMVFLEGGSFLMGNDQIPEESPTFEAHVD
                     PFYMDIHPVTNDQYREFLRATGHRKPEFWGDGTFGGGPQPVVGVSLEDALAYASWVGK
                     QLPTETQWEFAARGKDNRKYPWGNREPDPMLCNYGDMLSMTAILGMHEDGRTPEGVHD
                     LAGNVFEWTTGYYMPYQPGATEPKTPTIPRYVVRGGGWNSPPDELRCTYRKGLFPEER
                     LNTVGFRCVLPAVKNTAG"
     gene            2574..3581
                     /locus_tag="PKW60_11240"
     CDS             2574..3581
                     /locus_tag="PKW60_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HPK00051.1"
                     /translation="MKTYGFNEDLDLVIVGTGPAGLAALHAAQQAGLSAVALDKGPIC
                     SALMAHPTYMRWFSTFDKLELAGFPLVISEKNPTRREYLKYCRTFVQYFGLRVVTYCT
                     VAAVEPRSPGFTVRGRDLFERPYEWNARCVLIATGFYDSPRRLEVPGEDLPHVSHYYT
                     EGHRYAGQEVLVIGAGSSAAETALELYREGARVTVAMRGQDFQTKYWIEPDIENRIRE
                     GAITCHRGVQAVAIRPDEVELERHTGGRVVVPAEFVLALTGFVPDTSLLEAAGAAIDH
                     ETGKPVLSEAFETTVPGLYVAGTLCAGKEANVVFVENGREHGPVIVADILNKRKDGAA
                     M"
     gene            3597..>3916
                     /locus_tag="PKW60_11245"
     CDS             3597..>3916
                     /locus_tag="PKW60_11245"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HPK00052.1"
                     /translation="MPEIQWHLIEGAAGCAHAREHGLVAVVIDALRASATAAMLFDAG
                     ATELLVVREVAEAHAARAEFNDALLYGERGGLPPEGFDYGNSPRTVEAARGRRIIFTS
                     TTGAG"
BASE COUNT          749 a         1300 c         1135 g          732 t
ORIGIN      
        1 tgccgtcgac gctgatcgcc agtgcgcccg cgccgttcgc cgcggtcacc cggtgccctg
       61 aaggtatcag cgaaggcgtc aggaccatcg ccagcgtaag tgccacgaag gcgcccgcca
      121 gcgtcgtcag aaacagcctt ttcttactcc ccattgtcat tctcctctcc gtaacggttt
      181 tcagctcgaa gaccgatcat gaaaccacgc agaaaataca aaaattgccc ctgtccgttt
      241 cagataacac gaaccggact ctcctcgttc tgggcaattc caacctcttg cgtcttccca
      301 ttgccctgga gccccgctgc tccaggacat ctccccgacg ccgtatcgta ccagccccac
      361 aaacttatct tggagcgttc attatagata cggcgtcagc atgtgtcaac gatcatcccc
      421 gcgaagacag acaggggcag cagccacttt ctgcaccttt ttgcggccat tttccgcccg
      481 attttcccgt cgatttcccg ctcagccgcc gctcacccct caattgccgc gattgcgatt
      541 gcccagcgcc cctcccgcgt gctaccatag gtctgcctga actccaggcc ggaaaagcct
      601 tattccggat agacttagag gtcgctaacc cgaaatgggg gctgtcccgg gggtggcgta
      661 agcagttttc aagctcccgg gatcgtgatt attgtatcta tgcaacgagg cgcgagcatg
      721 ttgagatgat tcccgttacg tgttccagct gcggcctgcg aatattagtg cccaacgcgg
      781 tccaggggcg ctggggcctc tgtttcgggt gtggcgcccg catgatggtt ccacgcatcg
      841 ccaccgatcc caaagacaaa aacctgatct ttgaagccgg cgaacgcatt tctgatcggt
      901 acgaaatcga ggaattcctc ggccgcggtg gcatgggcgt ggtctatcgc gcccgcgacc
      961 acctcacgaa cgagcaagtc gccctcaagt tcatgatccc cgacctcctg aaatccccca
     1021 aagcacagca gttgttcatc caggaagcgc aggtggcgcg gcggctgcgg catgagaaca
     1081 ttgtggccgt ccatgacgtc agcacgaccc cggaaggcgt gctttacctc tcgatggagt
     1141 ttctgcgggg ccagtctctt cgcgggcttc tccgaaaata ccgcaaagaa cggcgcatgc
     1201 tggatgtgcg ggtggcggtg catgccaccg cccaggtgct caaagcgctc gattacgcgc
     1261 accgcatggt catccaccgc gatatcaagc ccgaaaacgc catgatgctt cccggtgaac
     1321 acatcaaggt gctggatttc gggctggcca aggccgttga caccgaagcc gccgccgccg
     1381 gggacacgca gcgcaagcgc gttgtcggca ccgcggccta tgcctcccct gaacagctga
     1441 acctgcgtcc ggtcgattcc cgcgccgacc tctactccac cggccttctg ttttacgagc
     1501 ttctcacgct gcgcgcccct accgaggagc agatcgcggt gaccgaggcg cgcagcgaca
     1561 tcgccccgtc gctcgtcgcc attctcaaca aggcgctcca ggaagacccc gcaaaccggt
     1621 ggcagaccgc gggcgagttc cgcgcccagc tgaccgaggc ctaccaggct tcctaccgcc
     1681 acgctgcccg gaccaggatc cccaccgtcg atcagacgcg cgaggtgtcc acggagggca
     1741 tggttttcct cgaaggagga agcttcctta tgggtaatga tcagattccc gaggaaagcc
     1801 ccacatttga ggcccatgtc gacccctttt atatggatat ccatcccgtc accaacgatc
     1861 agtaccgcga atttctacgc gccaccggcc accggaagcc tgagttctgg ggcgacggca
     1921 cctttggcgg cggcccccag ccggtcgtgg gcgtgagcct ggaggacgcc ctggcctacg
     1981 ccagctgggt tggaaaacaa ctccccaccg aaacgcaatg ggaattcgct gcccgtggca
     2041 aggacaaccg caaatatccc tggggaaacc gcgagcccga ccccatgctc tgcaattacg
     2101 gagacatgct gagcatgacg gccatcctcg gcatgcacga ggatgggcgc actcccgaag
     2161 gcgtgcatga cctcgccgga aatgtctttg aatggactac cggctactac atgccctacc
     2221 agcctggcgc caccgagccc aaaacgccca ccattccccg ttacgttgtc cgggggggcg
     2281 gctggaattc cccgcctgac gaactccgct gcacctaccg caagggcctc ttccctgaag
     2341 aacgcctcaa caccgtcgga ttccgctgcg tgcttcccgc cgtgaaaaac accgcgggat
     2401 aaaaaggcgc cgccccggcc atcctttgtt gcccacgcac gggggcaaag tccttccacg
     2461 tattccatac cttcccgggc ggaaccggta agactgcaat ccagcggagg ccggcctgac
     2521 tgttttcgca gtttgccccg gaagctgcta gaattcacct ttctggaggc gccatgaaaa
     2581 catatggctt taacgaggat ctggaccttg tgatcgtggg caccggaccg gcggggctgg
     2641 cggcgctgca tgcggcgcag caggccggcc tcagcgcggt cgcactcgat aagggcccta
     2701 tctgctccgc tctcatggca cacccgacgt acatgcgatg gttttcgact ttcgacaagc
     2761 tcgagctcgc gggtttcccg ctcgtcatct ccgaaaagaa ccccacgcgc cgcgaatacc
     2821 tgaaatactg ccggacattt gtgcagtatt tcgggctgcg cgtagtcacg tattgcacgg
     2881 tggccgccgt ggaaccccgc agcccagggt tcacagtgcg cggccgggac ctgtttgagc
     2941 ggccctatga gtggaacgcc cgctgcgtcc tcatcgcgac aggcttttac gattcgccgc
     3001 gccgcctcga ggtgccaggc gaagacctgc ctcacgtcag ccattattac accgaggggc
     3061 atcgttatgc cggccaggag gtgctggtta tcggcgcggg cagctccgcc gcggagacgg
     3121 ctctcgaact ctaccgggaa ggcgcccggg ttaccgtggc catgcgcggg caggattttc
     3181 agaccaaata ctggattgaa cccgacatcg agaaccgcat ccgcgaaggc gccatcacct
     3241 gccatcgcgg ggttcaggcc gttgccatcc gccccgacga agtcgagctg gagcggcata
     3301 cgggcggccg tgtcgtggtt cccgcggagt tcgtcctcgc cctgaccggg ttcgtgccgg
     3361 acacgtcact gctcgaggcc gccggggcgg cgatcgacca tgaaactgga aagccggtcc
     3421 tctccgaagc cttcgaaacc acggtccccg gcctgtacgt ggcgggaacc ctgtgtgcgg
     3481 gcaaggaggc caacgtggtg ttcgttgaga acggccgcga gcatggaccg gtaattgtcg
     3541 cggatatcct gaacaagcga aaggacggcg ctgcgatgta gcgcccgctt atggttatgc
     3601 ctgagattca atggcacctc atcgaaggcg ccgcgggctg tgcacatgcc cgcgaacacg
     3661 gcctcgttgc cgtagttatc gacgccctgc gggcgagcgc caccgccgcg atgctgtttg
     3721 acgcgggcgc caccgagctc ctggtggtcc gcgaggttgc agaggcccat gccgcgcgag
     3781 ccgaattcaa cgatgccctt ctctatgggg aacgcggcgg cttgcctccc gaagggttcg
     3841 actatggaaa cagccctcga acggttgagg ccgcccgcgg ccgccgtatc atctttacct
     3901 cgaccacggg ggccgg
//
LOCUS       DAOPBQ010000365         3916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18868_length_3916_cov_2.992748, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000365 DAOPBQ010000000
VERSION     DAOPBQ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18868_length_3916_cov_2.992748"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PKW60_11250"
     CDS             complement(<1..337)
                     /locus_tag="PKW60_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00053.1"
                     /translation="MDIPGANGAVTVCGIPVGSGRPVHITIRDGIIEAITQGGGYGRP
                     DYGTRSSIIGPTLFDIQVNGGNGINLQGRKTKPADVLELTRFLAAWGVSKWVPTITTN
                     TLANMEHACR"
     gene            complement(353..1534)
                     /locus_tag="PKW60_11255"
     CDS             complement(353..1534)
                     /locus_tag="PKW60_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPK00054.1"
                     /translation="MTSRKHRIRVRGILAGADAPSDLLLKDGQIQEIKLAGRGAPDIG
                     SDTCIIAPTLFDIQVNGAKGIDLQGDSVTPDDVCALTDFLVTQGVSHWIPTLITGPAE
                     HMRHGCAVIAAAMQNPRVQRAIPGIHLEGPFISPEDGARGAHDKNHVRPPSLEEFDGF
                     MKAADGHIAYITVAPEVEGAVDFIREVTRRKVRVALGHHNASSLEIERAVEAGAVLTT
                     HLGNGLVGTMDRHHNPLWPQLANDNLSASLIADLEHLPPPVLRAFVRAKEPQRVILVS
                     DCVHLAGLKPGEYELGGIPVELTPSGRICLSGTEYLAGSSLMLLQGVINAVRATDLTL
                     RQALASASSVPANLLGLKHPFRLPKTGTKANFMAFTITKGQEKWKTNIKAVFVDGEQR
                     A"
     gene            complement(1677..2492)
                     /gene="fetB"
                     /locus_tag="PKW60_11260"
     CDS             complement(1677..2492)
                     /gene="fetB"
                     /locus_tag="PKW60_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HPK00055.1"
                     /translation="MMEGSVPGAVALNSWQLVLASSFVLLAGGISFALRLGLEKRLAL
                     ASVRTVVQLLLAGLILRWVFQLEQLLPILAVILAMTAIAGHAAVQRSSRTFPGATRSA
                     VITLVLSTWLTAALVTGAIIQVQPWYKPQYLVPLLGMILGNALTGISLSLDQILQTLV
                     SKRDQVEMELAHGASRWEAARAPLSDAVRCGMIPIINSMMVVGLVSLPGMTTGQILAG
                     EDPFRAVKYQIVVMFMIAAATSLGCVLISVLVYHRVFNARHQLLSELIIKRHN"
     gene            complement(2489..3145)
                     /locus_tag="PKW60_11265"
     CDS             complement(2489..3145)
                     /locus_tag="PKW60_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK00056.1"
                     /translation="MVEPLLQLEKLAVAANGVPLLRDVSISLEAGERVALTGPSGCGK
                     TTLLRAVAGLIDPVAGRVRLRGQTPGTLGWPAFRRRVILVAQRPVLLEGTVRQNLSHP
                     FRYAATKAVFDESRALELMEQLLLEPSRMMQEALSLSEGQRQRICLLRALLVEPDVVL
                     LDEPCSALDAAAAEAVDEVVLREARRRGLAALIVSHERESIIARCDHIIDLTPAREAV
                     "
     gene            complement(3157..>3916)
                     /locus_tag="PKW60_11270"
     CDS             complement(3157..>3916)
                     /locus_tag="PKW60_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPK00057.1"
                     /translation="GLWIEPPNKPECRSLAGPIHNPAQALCELVAGMHDADGRVTLPG
                     FYDAVIPLDDDERRQIARWPMTDDGWKAMTGAPGLWGDAGYTTLERVGARPTLEVNGL
                     VSGFIGEGAKTVLPAKALAKLSCRLVPGQNPAAIKGMLEQYLSENAPDTVTWCVRELS
                     HGPGALMDRQSAPVRAAAKALKDVFGAEPVFRREGGSIPVVGVLKEKLGVDSVLLGFG
                     LPDDGIHGPNEKQHLPTLFKGIETYVRFLAGFQG"
BASE COUNT          751 a         1194 c         1226 g          745 t
ORIGIN      
        1 cccggcaggc atgttccatg ttggccaacg tgttcgtggt gatggttggc acccacttcg
       61 agacgcccca cgcagcgaga aaccgggtca gttcgagcac gtcggcgggc tttgttttcc
      121 gtccctggag gttgatcccg ttgccgccgt tgacttgaat gtcgaacagc gttggaccaa
      181 tgatcgacga gcgggtgccg taatccggcc ggccataccc tccgccttgc gtgatggctt
      241 cgatgatccc atcgcgaatg gtgatatgaa ccggccgtcc tgacccaacc gggattccgc
      301 atacggtgac ggctccatta gcacctggga tatccacgct cgtaacctct gtttatgccc
      361 tctgctcccc gtcgacaaac accgctttga tgttagtctt ccacttttcc tggccttttg
      421 tgatggtgaa tgccatgaaa ttggctttag ttcccgtttt tggaagccga aacgggtgtt
      481 taagccccaa aaggttcgcc ggtacggatg aggcgctcgc cagggcctgc ctgagggtca
      541 agtcggtggc ccgtaccgcg ttaatcacgc cctgcagcag cataagggag ctgccggcca
      601 gatattccgt tccactcagg cagatccgcc ccgatggcgt caattctacc gggattccgc
      661 ccagttcata ttcccccggt ttcaatccgg ccagatgcac gcaatccgat actagaatca
      721 cccgttgtgg ctctttggcc cgcacaaaag ctcgcaatac gggcggcggg aggtgttcga
      781 gatcggcaat gagtgacgcc gacaggttgt cgttcgccaa ttggggccag agcgggttgt
      841 ggtggcgatc catagtgccc accaggccgt tgccgagatg ggtggtcaag accgcccccg
      901 cctcgacggc gcgttcaatc tcgagtgagg acgcgttgtg gtggcccagg gcgacgcgta
      961 ccttccggcg ggtcacctct cgaataaaat ccactgcgcc ttcaacttcc ggggccacgg
     1021 tgatataggc aatatgaccg tccgcggcct tcatgaatcc gtcgaattcc tccagagacg
     1081 gcggccggac gtgatttttg tcgtgtgccc cgcgcgcgcc gtcttcgggt gagataaacg
     1141 ggccttcgag atgaatgccg ggaatggctc gctggacgcg cggattctgc atggcggctg
     1201 caattacggc acaaccgtgg cgcatgtgtt cggcgggccc ggtaatcagc gtgggaatcc
     1261 agtgtgagac gccctgcgtc acgagaaaat cagttagagc gcacacatcg tccggtgtga
     1321 ccgaatcgcc ctgcagatca atgccttttg cgccgttaac ctgaatatcg aagagtgtcg
     1381 gggcaatgat gcaggtgtct gagccgatat ccggcgcgcc gcggcccgcc agtttgattt
     1441 cctggatttg cccgtctttc aggagaaggt cggacggcgc atccgcgccc gcaagaatac
     1501 cccgtaccct gatgcgatgt tttctggaag tcacgtgccc gtctccttgc gattggcaag
     1561 ggttctacca caatcccgcc ccgggataca atacagaccg gcgcgccgcc ataccgtcat
     1621 cctggcgtcg aaacggcctc tgtttttcgt aaactgctga aaaataacac gttaagctaa
     1681 ttatgacgtt tgataatcag ctccgaaaga agctggtggc gggcattgaa cacacggtgg
     1741 tacaccaaca cggagatcag tacgcagccc agggaggtgg cggcggcgat catgaacatg
     1801 acgacgatct ggtatttcac agcccggaag gggtcttccc ccgcgaggat ctggcccgtt
     1861 gtcattccgg ggagagagac gagcccgacg accatcatgg aattgatgat gggaatcatg
     1921 ccgcagcgca cggcgtcaga cagtggcgcg cgcgcggctt cccagcggct tgcgccgtgg
     1981 gcgagttcca tttcaacctg gtcgcgtttg ctcacgaggg tttgcaggat ctgatcgagg
     2041 ctcaacgata tgccggtcaa ggcattgccg aggatcatgc ccaacagagg gaccagatac
     2101 tggggtttgt accagggctg gacctgaatg atcgcgcccg tgacgagggc ggccgtcagc
     2161 caggtgctga gaacgagtgt gatgacggcg ctccgggttg ccccgggaaa agtgcggctc
     2221 gaccgctgca cggccgcatg ccccgcaatg gcggtcatcg cgagaataac cgccaggatc
     2281 ggcaacagct gttccaactg gaagacccac cggaggatga gtccggcaag gagcaactgc
     2341 acgaccgtac gcacggaggc cagagccagg cgcttttcga gtccgagccg cagcgcgaaa
     2401 ctgataccgc cggcgagcag cacgaaactg gacgccagca cgagctgcca ggaattcaac
     2461 gcgaccgcgc ctggcacgga accttccatc atacggcctc cctcgccggt gtgaggtcga
     2521 taatatggtc gcaccgtgcg atgatggact cacgctcatg cgagacgatc agcgcggcca
     2581 acccacgccg gcgtgcttcg cggaggacca cctcgtcgac ggcttcagcg gctgcggcgt
     2641 caagggcgct gcagggttcg tcgaggagaa ccacatctgg ctccaccagc agggcacgga
     2701 gcaggcagat gcgctggcgc tgaccttcgg agagcgacag ggcttcctgc atcatgcggc
     2761 tgggttcgag aagcagttgt tccatcagct cgagggcgcg cgattcatca aagaccgctt
     2821 ttgtggccgc gtagcgaaat gggtgtgaca ggttctggcg gacggttcct tccaatagca
     2881 cgggccgttg cgcaacaaga atcacccgcc ggcggaacgc gggccatccg agcgtcccgg
     2941 gcgtctgccc gcgcaggcgg acgcgacccg caacgggatc gatcaggccc gcgacagcgc
     3001 gcagcagggt cgttttacca cagccggacg gacccgtgag cgccacccgt tccccggctt
     3061 ccagggagat ggagacatcc cgcaacaggg gaaccccgtt ggccgcaacc gcgagttttt
     3121 cgagttggag cagcggttcc accacctgct gcgcccctat ccttgaaaac cagccagaaa
     3181 gcgcacgtac gtctcgatgc ctttgaacag ggtgggcagg tgctgttttt cattggggcc
     3241 gtggatgcca tcgtccggca ggccaaaccc gagcaggacg gaatcgaccc caagcttctc
     3301 cttcagcacg ccgaccaccg gaatgcttcc gccctcgcgc cggaacacgg gctctgcgcc
     3361 gaacacgtct ttgagggcct tcgcggcggc gcgaactggc gccgactggc gatccatgag
     3421 cgcgccgggc ccatgcgaga gctcgcgcac gcaccacgtg acggtatccg gagcgttttc
     3481 agagaggtac tgctcgagca tgcccttgat tgcggcgggg ttctgcccgg gcacgagacg
     3541 gcacgagagc ttggcgaggg ccttcgccgg aagcacggtt ttcgcgcctt cgccgatgaa
     3601 ccccgacacc aggccattga cttcgagcgt ggggcgggct cctacgcgtt cgagggtggt
     3661 gtatcccgcg tcaccccaga gcccgggagc gcccgtcatg gctttccatc cgtcgtcggt
     3721 catcggccag cgtgcgatct ggcggcgttc gtcgtcatcg agcggaatga cggcatcgta
     3781 gaatccgggc agggtcacgc ggccgtcggc gtcgtgcatg ccggccacga gttcgcacaa
     3841 ggcctgcgct ggattgtgga tcggaccggc caaagatctg cattccggtt tgtttggcgg
     3901 ctcgatccac aatcca
//
LOCUS       DAOPBQ010000366         3907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18911_length_3907_cov_4.529076, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000366 DAOPBQ010000000
VERSION     DAOPBQ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18911_length_3907_cov_4.529076"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PKW60_11275"
     CDS             complement(<1..522)
                     /locus_tag="PKW60_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00058.1"
                     /translation="MDAAALTRVAEGFLKGCSDVIGAMLSTAPVFQVGEAEEAAEGTL
                     RELLAESPFAAQSALSGGGAVIFLAALDDARRVVSSVKGEAAVAAPTLSEDEVAAMTE
                     VFDPCMGGGVGQFKEQYGKTVTLQPVQIGLATPGGAAAMMTALGATPALVRFSYSVPG
                     VVDDGRGALLFSRT"
     gene            complement(538..1533)
                     /gene="fliM"
                     /locus_tag="PKW60_11280"
     CDS             complement(538..1533)
                     /gene="fliM"
                     /locus_tag="PKW60_11280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HPK00059.1"
                     /translation="MADILSQEEVDLLLNAVAEGDLDVEPKELERPAAELRDTTIYDF
                     RRPERVSKEQLKGLQSLFENFARELSIMLPPFLRTVVRVDLVSIDQLTYDEFILSVSR
                     PTALSVVNMNPLEGNCVIELSPPLVFPIVDRVLGGKGLPLAEPRELTEIENRIVHRIV
                     LMILDSLRRSWEQLIEFHLSVETQESDPLLVQIVAGSEMTILAGYELHIGECVGSMNM
                     CIPLVVLNPVFDQITQQTRFIRRLSPQVAAQTRAHIIRTAKRALTPVDAVLGHARMRL
                     DDVMRLRVGDVVQLDSNINEPIGVELGGLVRFKAHLGRRGEQSAVQISAVLHDDD"
     gene            complement(1787..2614)
                     /locus_tag="PKW60_11285"
     CDS             complement(1787..2614)
                     /locus_tag="PKW60_11285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPK00060.1"
                     /translation="MGNRLKNALKRGVKYGAATVGNFYPKRRGCSRILVYHGIGYRNH
                     EMNVTPEAFRAQMAWLVKNQTVISLADAARGVEGVAITFDDGYRDIWMNAAPVLQAFG
                     IPATVFIVPAGMGRRLSRKHDPVTDILLTWDEAKRLREVGIEIGAHALTHARLSELDE
                     SEQRREIGKCIRYIEKRLDETPRAFAYPRGSILDYNDLSKMLLEEFGFSVGVSTRYGV
                     NYPGCDPFDLHRIAIDSTDTLEGFQDKVTGRLDPLAVLESCAGAHVRRQVNRLLGTL"
     gene            complement(2708..>3907)
                     /locus_tag="PKW60_11290"
     CDS             complement(2708..>3907)
                     /locus_tag="PKW60_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00061.1"
                     /translation="TRALYLEHGGEAALIMGYDMCFLGREDADRFKGAVGRVMDLLPR
                     QIFLNTSHNHVGPKAGTWYSAGYEAPDRDYLNQLEQATVKAALAARAQARPVNMRAGV
                     TTSELPVNRRRRMPDGSIQNRPNPGGPTYNKLPIVLFEDPSGEVVCLLFSISCHPSMV
                     SGWEISAEYPGAAMDRLDAHFGAPCSLFLQGVGGDAKPLVIGRDTESWIPGTWELMGE
                     AGAIVAGEVLDAVGKGLAPVAPALASASEEMRWALEPAPGREFFEDIAANAPEEGRAR
                     DVKFMWARKTLEDLERGLPLPTDVPLLLHGIQLGDQLRIIGIEGEALHGWGYFIEDFY
                     GKKGVTIPLGYTDGQGMYLPLTEQLPEGGYEVISYWEYGYPSSLAPGMEEKVREALAA
                     LQNRGIS"
BASE COUNT          774 a         1286 c         1114 g          733 t
ORIGIN      
        1 cgtacgcgag aacagcaggg cgccgcgtcc atcgtccacg acgccgggca cgctgtagga
       61 aaaccggacc agcgccggcg tggcgcccaa cgccgtcatc atcgccgctg cgccgccggg
      121 agtggccagg ccaatctgca ccggctgcag ggtgaccgtt ttgccgtatt gttccttgaa
      181 ctggcccacg cctccgccca tgcacgggtc gaacacttcc gtcatggcgg ctacctcgtc
      241 ttctgacagg gtcggagccg cgaccgctgc ttctcctttg acggaggaca caacgcggcg
      301 ggcatcgtcc agggccgcca gaaaaatcac cgcaccgccg ccgctgagcg ccgactgcgc
      361 tgcaaagggg gattctgcca ggagttcgcg cagcgtaccc tcggcagctt cctccgcttc
      421 gccaacctga aacacaggtg cggtggaaag catggcgcca atgacatcgc tgcatccctt
      481 caagaagccc tcggcgaccc gtgtcaacgc ggcagcatcc atccgtccac tcccctgtca
      541 gtcatcatcg tgaagcacgg cgctgatctg tactgcgctc tgctcacccc gccgccccag
      601 gtgcgccttg aaacgcacca gtccccccaa ttctactcct atcggctcgt tgatattgga
      661 atccaactgg acgacatcgc cgacacgcaa tcgcatcaca tcgtccagcc gcattcgggc
      721 atgccccaaa accgcgtcca ccggcgttaa cgctctcttt gcggtccgaa tgatgtgggc
      781 gcgggtctga gcggcaacct gcgggctcag acgccggata aaacgggtct gctgggtgat
      841 ctgatcgaac acggggttta acaccaccag cgggatgcac atgttcatgg agcccacgca
      901 ttcgccaata tgaagctcgt atcccgccag aatggtcatt tcgctcccgg cgacgatctg
      961 gaccagcagc ggatcgctct cctgggtctc gacactcagg tgaaattcga tgagctgttc
     1021 ccacgaccgc cgcaggctgt ccagaatcat gagtacgatg cggtgtacga tgcggttttc
     1081 gatctccgtc aattcgcgag gctcggccag cggcagtcct ttccctccca gcacccggtc
     1141 tacgatgggg aacaccagcg gaggactcag ttcgatgacg caattgccct ccagggggtt
     1201 catattcacg acggagagcg ctgtgggacg cgacaccgac agaatgaact catcgtacgt
     1261 cagctgatca atagacacca ggtcgacccg caccaccgtg cgcagaaacg gcggcagcat
     1321 gatgctcagt tcacgggcaa agttctcgaa caggctctga agccccttca gctgttcttt
     1381 ggagacacgt tccggccggc ggaaatcgta gatcgtcgta tcccggagct ccgcggcagg
     1441 gcgttccagt tccttgggct cgacgtccaa gtcgccctca gccacggcat tcaaaagaag
     1501 atccacttct tcctggctca ggatgtcagc catgcggaag cccctgtgac gatagttttt
     1561 acagtcaata cctcaacttg gagtataaca cagcgcacag gagggcgcaa gaaaggctga
     1621 tcggcctaaa acatttatta ctaagtggat acacgcgaag aaccgtccgg gcgaagtggc
     1681 agaaaaaagc gtgaccctcc cgtgaaacgc gctatcatca tcttcgtcaa taaattactg
     1741 tggtacggtc gaatagcttc cccgccggct ccccgccccc atgtgctcac agggtaccca
     1801 gcaggcggtt cacctgccgc cgcacatgcg ccccggcaca cgattccagg acggccagcg
     1861 gatccaggcg gccagtcacc ttatcctgaa aaccctccag agtatcggtg gaatcgatgg
     1921 caatccggtg cagatcaaag gggtcgcagc ccggataatt cacgccatac cgggtgctta
     1981 cgccaacgct gaaaccaaac tcctccagca acatcttcga caggtcgttg taatcgagga
     2041 ttgagccgcg gggataggca aacgcccgtg gggtctcgtc gaggcgcttt tcaatgtagc
     2101 ggatgcattt tccgatttca cggcgttgtt cgctctcatc gagctcggaa agccgggcgt
     2161 gcgtgagcgc atgggcgccg atctcgatgc cgacctctcg aagccgtttt gcctcatccc
     2221 acgtcaaaag aatgtcggtc acggggtcgt gcttgcgcga cagccgtctg cccattccgg
     2281 cgggcacgat aaacaccgtg gcggggatgc cgaaggcctg cagcacgggc gcggcattca
     2341 tccagatgtc gcggtagccg tcgtcaaagg ttatcgcgac gccctcgact ccccgggccg
     2401 catcggcgag cgagatcacc gtctgatttt tcacgagcca ggccatctgc gcccggaacg
     2461 cttccggcgt aacgttcatc tcgtgattcc ggtagccgat gccgtgatag acgaggatac
     2521 gcgagcagcc gcgccgtttt ggatagaaat ttcctacggt tgccgcgcca tatttcacgc
     2581 cgcgtttcag cgcgttcttc agccggttac ccacgcccaa ccgcggactc cttcgctggc
     2641 aacgtttcct gcagcaggcc gcggtggttt tgcgcaagcc ggccatccgg cagatgcgcg
     2701 caccggctca tgagatgcca cggttctgaa gggctgccag cgcctcgcgc accttttctt
     2761 ccattcccgg cgcgagcgat gaggggtagc cgtattccca gtaactgatc acctcatagc
     2821 cgccttccgg caactgttca gtaagcggca ggtacatgcc ctggccgtcg gtgtatccca
     2881 ggggaatggt cactcccttt ttcccgtaaa aatcctcaat aaaatacccc catccatgca
     2941 gagcttcgcc ctcgatcccg atgatgcgca gctgatcacc cagctggatg ccgtgcagca
     3001 acaacgggac atcggttggc agaggcaggc ccctctcaag gtcttcgagt gtcttacgcg
     3061 cccacatgaa cttaacatcg cgcgcccgcc cttcttcggg ggcattggcg gcgatgtcct
     3121 cgaagaattc tctgcccggg gctggttcga gagcccagcg catctcctct gatgccgagg
     3181 ccagcgcggg cgcgaccggc gccaggcctt tcccgacggc atcaagcact tcgcccgcga
     3241 cgatagcgcc ggcttcaccc atgagctccc aggtcccggg gatccagctc tcggtatcac
     3301 ggccaatcac cagcggcttg gcatcgccac cgacgccctg cagaaagagg ctgcacggcg
     3361 ccccgaaatg cgcgtccagc cggtccatgg ccgcaccggg atactcggcg gagatctccc
     3421 agccgctaac catggagggg tggcaggaga tggagaacaa gaggcacacg acttcgcccg
     3481 agggatcctc aaacagcacg atgggaagct tgttgtaggt cggcccgccc ggattggggc
     3541 ggttctggat gctgccatcg ggcatgcggc gccggcggtt gaccggcaat tccgaggtag
     3601 taacgcccgc gcgcatgttc acgggccggg cctgcgcgcg cgccgcgagc gccgccttca
     3661 ccgtcgcctg ctcgagctgg ttcaaataat cgcggtccgg cgcttcgtat cccgccgaat
     3721 accaggtccc ggctttcggg cccacgtggt tatgcgacgt attgaggaag atctggcgcg
     3781 gcagcagatc catcacccgc ccaacagcac ctttgaaccg gtcggcatct tctcgaccca
     3841 gaaagcacat gtcgtagccc atgatcagag ccgcttctcc cccatgctcc agatacaggg
     3901 cccgcgt
//
LOCUS       DAOPBQ010000367         3896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18961_length_3896_cov_4.773236, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000367 DAOPBQ010000000
VERSION     DAOPBQ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18961_length_3896_cov_4.773236"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PKW60_11295"
     CDS             complement(<1..1159)
                     /locus_tag="PKW60_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPK00062.1"
                     /translation="MTTMLRTVLILTLLGLAAFAARSEDAPDYDCQVVWREPGAGGFS
                     AANALFPGGSGGASALVVCEGDTGVVCIALDGKRLWSYPLTPPVTATPAVADIDGDGI
                     EDIVAGDSVGTLVALRADGSLMWKAEVPGRIMADSGPSIADLDGDGRAEVLVGDASGA
                     LSCFDHTGALRWRFAGDGSRMGPVLVADIYDTPGQEIIVTSHDSHIYALTSNGEWLWD
                     LYFEDDLFPNSTPILADVNGDAVPELYIGGGLHHFYAIDLERVAVKVAENVYLHVNGC
                     IAAGDVNGDGLDEVLFGNKGGALWCYGKDGFHWTRQFGDCGFYAAPTLLDLNGDSLPE
                     IIMPSIEGQLLLLNSDGTKLLAAGLGCKTATTPLVGDLDGDGLPDLIINMSY"
     gene            1474..3486
                     /locus_tag="PKW60_11300"
     CDS             1474..3486
                     /locus_tag="PKW60_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00063.1"
                     /translation="MVHGSVSRLAALLMVSCLSVLAALAEDPNPPTIHSNSGAWPIRR
                     QWTPAETHHYARWIENIYLMKTQGTVEQRTAKLQRILTDPEMNLLEDPEFAGKGSNPQ
                     LPAPVIRAMHYLMDCGKFTAFMPAYYAYRRALPWMSTVVTAGQGDIRTSPSNIPVGSV
                     NSFEYRSLGDFFNHSVGWFISGNYRVNLNERRAEWSDTVPVAINRDYLLPGCVNYVDG
                     HCLLLAHVSEYGELYFLNCSTTITRDIFSYNGMNTVSGITPHGSDPDNPWNGCFQGMR
                     VFRYPIAETDSRGRVTRVRRRTDEEMREFGFSTEQYEIIQEIYTNQSITEGDLKPQSF
                     HDLIRLRMKTVDSISPLKFMEQYADELLEVYATREDFVQDAWRDVRTNGGITYPEERP
                     DENIFQALGRWETWSSPSSDVDRRNKYFYLADWMEYAIRLYGLVPSFVELAGLEKYEI
                     HSQSDLAKALLAEKNRLFAEHRMEYTNSAGKKVTLTLLDIEERLYDLSFDPNHPPELR
                     WGAPEGSEERASAPETFTPVPGGARVAMEDGYRWQAFYRSLGQRETELSCLRGMFTSG
                     YPIRDKLDAQVGKWIQEPEPAPEEPARLADTAEEPEAAATGLAETGSLSAETQAQAPD
                     GGTLPRLSDDSGREGAWTFTEAAHESGSSVKKREPEAARRRYPRRH"
     gene            3574..>3896
                     /locus_tag="PKW60_11305"
     CDS             3574..>3896
                     /locus_tag="PKW60_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00064.1"
                     /translation="MGLILLPVSVFAADPNPPSIHSERGAWPIRRQWTFAETRHFAQW
                     IQHIYKAKTQGTEEQRLARIERILTDPEMNLLLVPEFAGEGCNDQLGPGTLRAMHGVL
                     DCAKLT"
BASE COUNT          795 a         1211 c         1165 g          725 t
ORIGIN      
        1 gatatgacat gttaataatc aaatccggga ggccatcgcc gtccaggtca cccaccagcg
       61 gagtcgtggc ggttttgcag cccaggccgg cggccagaag tttcgtgccg tcgctattga
      121 gaagcaacag ttgtccctca atcgacggca tgatgatctc gggcagcgag tcaccgttca
      181 gatcgagcag cgtcggagcc gcataaaacc cgcagtcacc gaactggcgg gtccagtgaa
      241 acccatcttt gccgtagcac cataatgcgc cccccttgtt gccaaaaagc acttcgtcga
      301 ggccatcgcc attcacatcc ccggccgcga tgcagccgtt cacgtgaaga tagacatttt
      361 ctgccacttt gaccgccacg cgttcgagat cgatggcgta aaaatggtgc aatcctcccc
      421 cgatgtacaa ctcgggcacg gcatcgccgt tgacatcagc caggatcggc gtactgttgg
      481 gaaacaggtc gtcttcaaaa tagaggtccc agagccactc gccgttcgac gtcagggcgt
      541 agatgtggct gtcatgcgag gtgacgatga tctcctggcc gggcgtgtcg taaatgtccg
      601 caaccagcac cggccccatg cggctgccgt ctcccgcgaa gcgccagcgc agcgcgccgg
      661 tatggtcaaa gcacgacaag gcgccgctgg cgtcgcccac cagcacctcg gcacgcccat
      721 cgccgtccag gtctgcaatg gagggtcctg aatccgccat gatccgcccc gggacctcgg
      781 ccttccacat gaggctgccg tcagcgcgca gggccaccag cgtgccgacg ctgtccccgg
      841 caacaatatc ttcaatgccg tcgccgtcaa tatcggcaac cgcgggggtc gccgtcacgg
      901 gcggggtcag cggataactc cagaggcgtt ttccgtccag cgctatgcat acgacccccg
      961 tgtcaccctc gcagaccaca agggcggagg ctcccccgct tcccccgggg aacagggcat
     1021 tcgcggcgct gaatcctccc gcgccaggct cacgccagac cacctggcag tcataatcgg
     1081 gcgcatcttc cgaacgggca gcaaatgccg ccagcccgag tagcgtcaga atcaataccg
     1141 tacgcagcat cgttgtcata gccatactcc caggaattgt ctctgcttgg gttgggtgcc
     1201 tatgatactc gaatttggag atgaaagagg aatttgtgtc tggcggcgag tcattctgcg
     1261 gcgaatgcgg ggcttcgtgt cccaggttgc gagagggatt ttcgcgccgc tcgtatgcgt
     1321 tgagtataat acgaacacaa gagtgtttgt ttgggaaata tcggatacgg cggtggcagg
     1381 gggccgccct gatttccgcg cctgcctacg ccgcttgcgc tgacagtacc gcgccgcggg
     1441 ccgctgcggc gcgatgcaca atgaggacat ttgatggtgc acggtagtgt ctcgcgcctc
     1501 gctgcgttac tgatggtgtc ctgcctgtcc gtgctggccg ccctcgcgga agaccccaat
     1561 ccgcccacta tccactcgaa ctccggcgcg tggccgattc gccggcagtg gacgcccgcg
     1621 gaaacccacc attacgcccg ctggatcgaa aatatttatc tgatgaaaac ccagggaacg
     1681 gtggaacagc gcacagccaa gctgcagcgc attctgaccg acccggaaat gaacttgctc
     1741 gaagatccgg agtttgccgg aaaaggatcg aatccgcaat tgcccgcacc agtgatccgc
     1801 gccatgcatt acctgatgga ttgcggaaaa ttcaccgcgt tcatgccggc ctattacgcg
     1861 taccgccgcg ccttgccgtg gatgagcacc gtcgttaccg cgggccaggg cgatatccgg
     1921 acatccccgt ccaacatacc cgtgggcagc gtcaacagtt ttgaataccg gtccctgggc
     1981 gactttttca accactcagt aggctggttc atctcgggca attaccgcgt caacctcaac
     2041 gagcgccggg cggagtggtc tgacaccgtt ccggttgcga tcaaccggga ctacttgctc
     2101 ccgggctgcg tcaactacgt tgacggccac tgcctgctgc tggcccacgt ctcggaatac
     2161 ggcgaactct attttttgaa ttgcagcaca acgatcaccc gcgacatctt ttcctacaac
     2221 gggatgaaca cggtgtcggg catcacgccg catggcagcg atccggataa tccctggaac
     2281 gggtgcttcc aggggatgcg tgtgtttcgc taccccatcg cggaaacgga ttcccgcggg
     2341 cgggtgactc gcgtgcgccg gcgaaccgac gaagagatgc gggagttcgg cttctcgacc
     2401 gagcagtacg agatcatcca ggaaatttac acgaaccaga gcatcaccga gggcgacctg
     2461 aaaccccaga gttttcatga cctgatccgc ctgcgtatga agaccgtgga ttccatttcc
     2521 ccgctcaagt tcatggaaca atatgcggac gaactcctcg aagtgtatgc cacgcgcgag
     2581 gattttgtgc aggacgcctg gcgcgacgtg cggacgaacg gtggcattac ctatcccgaa
     2641 gaacggcccg atgaaaacat ttttcaggca ttgggacgct gggagacctg gagctctccg
     2701 tcgtcggatg tcgatcgccg caacaagtac ttttacctgg ccgactggat ggagtatgcg
     2761 atccggctct atgggctggt gccgtcgttc gtggaactgg ccggcctcga aaaatacgag
     2821 atccacagcc agtcggacct ggcaaaagcg cttctcgccg agaaaaaccg cctctttgcc
     2881 gagcaccgca tggagtacac caattccgcc ggcaagaagg taacgctgac gctcctcgat
     2941 atcgaggagc ggctgtacga cttatcgttc gacccgaacc acccgccgga gctgcgctgg
     3001 ggcgctccgg aaggaagtga agagcgcgca tcggcgccgg agacctttac gccggtgccc
     3061 ggaggcgccc gggtggccat ggaggacgga taccgctggc aggcgtttta ccgctccctg
     3121 ggccagcgcg aaacggaatt gagctgtctg cgcggaatgt tcaccagcgg gtaccccatt
     3181 cgcgacaaac tcgatgcgca agtcggcaag tggatccagg aaccggagcc ggcgcctgaa
     3241 gaacccgctc gtctcgcaga cacggcggag gagccggagg ccgcggcaac cggtttggcc
     3301 gagactggct cgctttccgc tgaaacgcag gcgcaggccc ccgatggcgg gaccttgccg
     3361 cgtctcagtg acgattccgg gcgtgagggc gcctggacct tcacggaggc cgcgcatgaa
     3421 tccggctcat ccgtgaaaaa gagagaacct gaggccgcaa ggcggagata tccgcgacgc
     3481 cattgacgat gtgaagggaa aacgcctgtg aatcacgttc gacctccggc gccgggggcc
     3541 cgtccgttga gagggtctct gtatgcagtg tgcatgggac tgattttgct tccggtctcc
     3601 gtctttgcgg cggaccccaa tccacccagc atccattcgg agcgcggggc ctggccgatc
     3661 cggcggcagt ggacctttgc ggagactcgc catttcgctc aatggatcca gcacatttac
     3721 aaggcaaaaa cgcaaggcac cgaggagcag cgtctcgcac ggattgaacg gatcctgacc
     3781 gatcccgaga tgaatctgct gctcgttccc gagttcgcgg gcgaagggtg caacgaccag
     3841 ctcgggcccg gcaccctgcg cgccatgcac ggtgtgctcg attgcgccaa gctgac
//
LOCUS       DAOPBQ010000368         3894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18971_length_3894_cov_3.382912, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000368 DAOPBQ010000000
VERSION     DAOPBQ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18971_length_3894_cov_3.382912"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="PKW60_11310"
     CDS             complement(<1..1431)
                     /locus_tag="PKW60_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HPK00065.1"
                     /translation="MADNARKKTVHMIGHGHIDPTWLWRWTEGYEEVRATFRSALERM
                     NETPDFRFTASSACFYAWIRDCEPAMFEEIRRRVNEGRWEIAGGWWVEPDCNLPEGES
                     FVRHGLYSQRFLQREFGIRARVGFNPDSFGHAGTLPQIYKKMGIEYYAYMRPDATREM
                     DYPDGTTFWWEAKDGSRILACNLQESYNADQETLERIARIADSPHLNPGQTHVLAFYG
                     VGNHGGGPTKRAIGHILEAQRRPDMPHTLFSTVMDYFQEFLAASADDSIPVITRDLQH
                     HARGCYSVHTEVKRINRRAEHALMIGERLAAMATLLAGHPFPKDRIEKAWHYVLYNQF
                     HDILAGTSLESSYEDSRDQQGAARTAGIEIANEALQVIASRIDTTAEGNTVVAFNPLP
                     WPVKETLVAPQNAARTLDEPLHLADEHGQPMPVQAVPGERIGHVNYSFTAELPAFGYR
                     CFHFRSGAKTPELSGMLESGRDFLEND"
     gene            complement(1504..2241)
                     /locus_tag="PKW60_11315"
     CDS             complement(1504..2241)
                     /locus_tag="PKW60_11315"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HPK00066.1"
                     /translation="MGYLDDFFAAANSVFEKIRTTQRSAIESAAEAIAASLADGGAVC
                     VMDTGHMLKHEAFNRAGGLMALAPFSYALDVENPLTQRTEARSEEENAEFEARLVALA
                     LDRSLLASGDVLIINSNSGRTPNVIETALQCRERGIVTIGISSSEQMRGCTAAHPSGK
                     KLFDVVDVALDNCAPFGDAAVEVKDNEKMCPMSGMAGAYCLWAVHAEAVERLQARGIN
                     PTIFRSVHVSGGGFVEEQRKKFLERGV"
     gene            complement(2241..3179)
                     /locus_tag="PKW60_11320"
     CDS             complement(2241..3179)
                     /locus_tag="PKW60_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPK00067.1"
                     /translation="MAATRKELWLAVEGGGSKTRLLLADTERKILAREVGGTSSGLYV
                     QAPDYDAYAARMRALLQNILATVDAVEGRVTVVGLAAPMVKELVARLVREILGDVAIV
                     ITSEAAVALACYDLRAGVSLVAGTGASCVALNEQGEGAACGGFGPQFGDEGSGYWLGR
                     EAIAAAMREKDGRGPKTALSSALCAFYQIGHIMEIYTFRDPGGHVSGPKVASCVPVVF
                     ETARAGDAVARAVCRRAGHALGHTVVATVKRAGIVERPVPVVLTGGVFNGGALLLTPL
                     KRVLRRSGIEFRVFPVVTEPGEAIFKIIERQRQGVF"
     gene            3565..>3894
                     /locus_tag="PKW60_11325"
     CDS             3565..>3894
                     /locus_tag="PKW60_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HPK00068.1"
                     /translation="MNTEEITQAFQQAAALFQAQQYEQALQLLEQLDNAAPNTRDILY
                     FKARVLGVLNRPQESVALCDRLTALGDARGEQLKAALAVAPAGPPPLPTTEDDTAAAP
                     TPLLPDQD"
BASE COUNT          718 a         1278 c         1163 g          735 t
ORIGIN      
        1 gtcgttttca agaaaatcgc gcccggattc aagcatgccg gaaagctccg gtgtttttgc
       61 gcctgaccgg aaatgaaagc agcgataacc aaaggccggc agctcggccg tgaagctgta
      121 attcacgtgg ccgatgcgct caccgggcac ggcctgcacg ggcatgggct gtccgtgttc
      181 atcggccagg tgcagcggtt cgtcaagcgt gcgcgcggcg ttctgcggcg ctacgagggt
      241 ttctttcacc ggccagggca gcggattgaa cgcaacgacg gtattgccct ccgcggttgt
      301 gtcgatccgg ctggcgatca cctgaagcgc ttcgttggcg atctcgatgc ccgccgtgcg
      361 cgcagcaccc tgctgatccc gcgaatcctc ataggacgac tccaggctgg tcccagcgag
      421 gatgtcgtgg aattggttat acagcacgta atgccacgcc ttctcgatcc ggtctttcgg
      481 aaatggatgt cccgcgagca gagtcgccat ggcggccaga cgctccccga tcatcagcgc
      541 atgttccgcc cggcggttta tccgtttaac ctccgtgtgg acgctgtaac aaccccgtgc
      601 gtgatgctgc aggtcgcggg taatcacggg gatggaatcg tccgcggatg cggccaaaaa
      661 ctcttggaaa taatccatca ccgtggagaa cagcgtatgg ggcatatccg ggcggcgctg
      721 ggcttcgagt atatgcccga tcgcgcgttt ggtgggccct ccgccgtggt tgcccacgcc
      781 gtaaaacgcg agcacatggg tctggccggg attcaaatgc ggggaatcgg cgatccgggc
      841 gatccgctcc agggtttcct gatcggcgtt ataggattcc tgcagattgc acgccaggat
      901 gcgggaaccg tccttggcct cccaccagaa cgtcgtgccg tcggggtaat ccatttcgcg
      961 cgttgcgtcg ggacgcatgt aggcgtagta ctcgatcccc atctttttgt agatctgggg
     1021 cagagtgcct gcatgtccaa agctgtcagg attgaatccc acgcgcgccc gaataccgaa
     1081 ttcgcgctgc aggaagcgct gcgaatacag gccgtgacgc acaaacgatt cgccttcggg
     1141 caggttacag tccggctcca cccaccaccc gccggcgatt tcccagcgcc cttcgttgac
     1201 gcgccggcga atctcctcga acatggccgg ctcgcagtct cgaatccatg catagaaaca
     1261 ggcgctgctc gccgtaaacc ggaaatccgg tgtttcgttc atgcgttcga gagcgctgcg
     1321 aaaggtcgcg cggacctctt cgtagccttc ggtccagcgc cacagccacg tcgggtcgat
     1381 gtgcccgtgg ccaatcatgt gaacggtctt tttgcgtgcg ttgtccgcca tgaaatcccc
     1441 ctgcctttac accttgccgg tatgcgtaac cttcaccggg agcgggcgcc gggacccaga
     1501 gagtcaaacg ccccgctcca ggaacttttt ccgttgctct tcgacaaatc cgccgccgct
     1561 gacgtggacg ctgcggaaaa tggtcggatt gatgccccgc gcctgcaatc gttcgaccgc
     1621 ttcggcatgc acagcccaca ggcagtacgc gccggccatc ccgctcatcg ggcacatctt
     1681 ttcgttgtct ttcacctcta ccgcggcatc gccgaacggt gcgcagttgt cgagggccac
     1741 atccaccaca tcgaagagtt ttttaccgga tggatgggcg gcggtacatc cacgcatctg
     1801 ctcggacgac gagatcccga tcgtgacaat gcctcgttcg cggcactgca gggcggtctc
     1861 gatcacgttg ggcgtccgcc cggaattgga gttgatgatg agcacgtcgc cggaggcaag
     1921 caggctgcga tccagtgcaa gggcaacgag ccgggcttca aattccgcgt tctcttcttc
     1981 ggagcgcgct tctgtccgct gcgtcagggg gttctcgacg tccagcgcat aagagaacgg
     2041 ggccagcgcc atcagcccgc cagcgcggtt gaaggcttcg tgtttcagca tgtggcccgt
     2101 atccatgacg cacacggccc cgccatcggc caggctggcc gcaatggcct ccgcggcgct
     2161 ttctatggcg gaccgctgag tcgttcgaat cttctcaaac acactgttcg ccgccgcaaa
     2221 gaaatcgtcg agatatccca ttagaaaact ccctggcgct gtcgttcgat tatcttgaat
     2281 atggcctccc cgggctccgt caccacaggg aatacgcgga actctatacc acttcggcgg
     2341 agtacccgtt tgagcggggt caggagcagc gcgcctccgt tgaacacgcc gcccgtcaac
     2401 acgacgggga cgggccgttc cacgatgccc gcgcgtttga ccgtcgcaac aacggtgtgc
     2461 cccagggcat ggcccgcgcg ccggcacacg gcccgcgcga ccgcatcgcc tgcgcgggct
     2521 gtctcaaaca cgaccggcac gcaggacgcc actttcggtc cggacacgtg gccgcccggg
     2581 tcccggaacg tgtagatctc catgatatgc ccgatctggt aaaacgcaca cagcgcgctg
     2641 ctgagcgcgg ttttcgggcc ccggccgtct ttttcgcgca tcgccgcggc gatggcttcc
     2701 cggccaagcc agtagccgct gccctcatcg ccgaactgcg gcccgaaacc gccgcaggcc
     2761 gcgccctctc cctgttcgtt cagggcgacg cagctcgcgc cggtgccggc caccaggctg
     2821 acgcccgcgc gcagatcata gcaggccagc gcgacggctg cctcggacgt gatgacgatg
     2881 gccacatcac ccaggatctc gcggaccagc ctggccacca gttccttcac catgggcgcg
     2941 gccagtccaa caaccgtaac acggccttcg accgcatcaa cggtagccag gatattctgc
     3001 agcagggcac gcatgcgcgc agcgtacgca tcgtaatccg gcgcctggac gtacaatccg
     3061 gaggaagtgc cgccgacttc gcgggccagg atcttgcgct ccgtatccgc aagcagcagc
     3121 cgggtcttac tgcctccgcc ttcaacggca agccacagtt ctttccgggt agcagccatg
     3181 gtcgtggtgg tcaatcctgc gaaaaccctt ctgcccggcg gccggagcgg accgcggcgg
     3241 cattgtaggt acggcaacgc gcgatttcaa ccgctgcagc gcctcgcgcc ggtaacggcc
     3301 gtttagagcc tgagggaatg ctctggctta cgggaccgtt cgccggcatc gcttcccagg
     3361 gcgtccggaa ccggcattcc aataggaaaa atgacgtggg ctcccggccg cttgccgtgt
     3421 tttcactcaa agagcacaat tttccttttt tttgaatcca cagcctgcat tggcctacca
     3481 tgagccgata gagcgtgtcc aacgcaggcc ggtgggccgg ccggcgcaac ctttgttgcc
     3541 tgaaaccaag gtggttgaaa cacgatgaac actgaagaga tcacacaggc atttcagcag
     3601 gcggcggccc tttttcaggc acaacagtac gagcaggcac tccagctcct ggaacagctc
     3661 gacaacgcgg cgcccaacac acgcgacatc ctgtatttca aggcacgagt gctgggcgtg
     3721 ctcaaccggc ctcaggaatc cgtggcgctc tgcgatcggc tcacggcgct cggagacgcc
     3781 cggggcgagc aattgaaagc tgcgctcgcg gtagcccccg cgggaccgcc gcccctgccc
     3841 acaacagagg acgacaccgc tgctgccccg acccctctcc tgccggatca ggac
//
LOCUS       DAOPBQ010000369         3889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_18998_length_3889_cov_4.000782, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000369 DAOPBQ010000000
VERSION     DAOPBQ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18998_length_3889_cov_4.000782"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PKW60_11330"
     CDS             <1..619
                     /locus_tag="PKW60_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPK00069.1"
                     /translation="PSAGGQGGNLEVDVNFMFRVPLWPVQRIDSRPVGIWQAAGIPVV
                     DVHELAAGKLSALLSRRQARDLFDCSNLFRLGGFDRERLRLASVVYGAMSRKDWRTIA
                     LVDVDVDTADLEQKFIPTLRSAGSDEIRKDRFGKGRLVEDCCNLLASLLPFTAPEQEF
                     LDRILDRGEIAPELLTDDESLQNSIRHHPLLEWKAVNVRGHKKGR"
     gene            complement(824..1468)
                     /locus_tag="PKW60_11335"
     CDS             complement(824..1468)
                     /locus_tag="PKW60_11335"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HPK00070.1"
                     /translation="MTCVKWLSLIRLFKVEVSVRKMSQQMGLSYRAVYRAVSAIHFSI
                     LSHAGDAERLLGGEIELDESYFGGKRKGKRGRGAAGKVPVFGILERDGVVQVTVVPDV
                     SAETLLGLTVKKVRRGSVVYTDKFKSYDSLMFCGYRHLSVDHQKRFSSGKAYINGLEG
                     FWSWASERFIKHHGVSKEHFPLYLKDLEFRYNNRHSDIFDKIANYLCDPVPKRD"
     gene            complement(1898..>3889)
                     /locus_tag="PKW60_11340"
     CDS             complement(1898..>3889)
                     /locus_tag="PKW60_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HPK00071.1"
                     /translation="TVLKVRNRTDQPRWLDVTGYAEWVLGDLRPKTMLHVITEVDPQS
                     GALLARNPYHTEFGDRVAFFNVDDGTRTVSGDRTEFLGRNGTPRSPAAMTRARLSGKT
                     GAALDPCGAMQVAFELAGGEERQIVFTLGAGRDADDARGLIRRFGGAGAARAELEAIW
                     QYWNHTLGAVQVETPDPSINVLANGWLVYQTIACRLWARSGYYQSGGAFGFRDQLQDV
                     MALVHAEPRLMREHLLLAASRQFAEGDVQHWWHPHAGRGVRTHCSDDFLWLPLAACRY
                     VLATGDNGVLDEPVRFLKGRPVNPEEDAYYDLPHTSEETASLYDHCVRAVRRGIQRTG
                     EHGLPLIGSGDWNDGMNMVGKDGKGESVWLGFFLCEVLMQFSGIALRRGDRPVAEQCR
                     QAVAELRRNIEQHGWDGGWYLRAYFDDGSLLGSAGNTECRIDSIVQSWSVLSGAGDAG
                     RASTAMEAVGKHLVRRDDRLVQLLDPPFDKSDSNPGYIKGYVPGVRENGGQYTHGAIW
                     AAMAFAAMDDGRHAWELLAMINPVNHGRSAEEIETYKVEPYVMAADVYARPPHTGRGG
                     WTWYTGAAGWMYRLILESLLGIRLDVDRLHLSPSLPSDWKAFKMHYRYRETVYHISVL
                     QGRAGEDRTSITVDGVVRYDNIVPLFDDRREHFVEVRMA"
BASE COUNT          784 a         1190 c         1080 g          835 t
ORIGIN      
        1 tcccagcgcc ggcggccagg gaggcaacct ggaggtggat gtcaatttca tgtttcgggt
       61 ccccctgtgg ccggtgcagc ggatcgattc acgaccagtc ggcatctggc aggctgcagg
      121 catcccggtg gtggacgtcc acgaactcgc ggctggcaag ctctcggcgc tcctctcgcg
      181 acggcaggcg agggatctgt tcgattgcag caatctcttc cgtctgggcg gcttcgatcg
      241 ggaacggctg cgtttagcct ctgtcgtcta cggcgccatg agccgcaagg actggcgaac
      301 cattgcactg gtggatgtgg atgtcgatac cgccgatctg gaacagaagt tcattcccac
      361 gctaaggtcc gcagggagcg atgagatccg gaaggatcgc ttcggcaagg gccgccttgt
      421 cgaggactgc tgcaatcttc ttgccagcct gctgcccttc accgcgccgg agcaggaatt
      481 tctcgatcgg attctggaca ggggcgagat agcccccgag ttgctgaccg acgatgagtc
      541 cctacagaac agcatcaggc accacccgct tctggagtgg aaagctgtga atgtaagagg
      601 ccacaagaag ggtcgctgaa gttcttagga gtgatgtcca gccttcacca cccaattttt
      661 tggtcattac tttggtcaat acttttcccc aaaaacctcc agatacggtc accttgcgac
      721 cgtcaactcc gccatgcttc caatcagttc gttggcgata ttgctgcttt ccagccgcat
      781 gatactttct tccgagtgtc cgaaactcaa aagatttagg taattagtcc ctttttggca
      841 ccggatcaca gaggtagttg gctatcttat caaagatatc ggagtgacga ttgttgtacc
      901 tgaactccag atctttcaaa tagagaggga aatgttcctt ggagacgcca tggtgcttga
      961 tgaagcgttc gctggcccag ctccagaatc cctctaagcc gttgatgtag gcctttccgg
     1021 aagagaagcg tttctgatgg tccacactca gatgccggta cccgcagaac atgaggctgt
     1081 cgtagctttt aaacttgtcc gtatagacga cggaacctct gcggaccttt ttgaccgtga
     1141 gccccagcag ggtttcggcg ctcacatcgg gaaccacagt gacctgaacg acaccgtctc
     1201 tctccaggat tccgaagacg ggaaccttgc cggcagcacc gcgaccgcgt ttccctttgc
     1261 gtttaccccc gaagtagctc tcatccaact cgatctcgcc acccagtaat ctttcggcat
     1321 ccccggcatg gctcagaatc gaaaaatgga tggcgcttac ggcccgatac accgcccggt
     1381 aggacaaacc catctgctga gacatcttcc ggactgaaac ctctacctta aagagcctga
     1441 tgagagacaa ccactttaca cacgtcaatc gcccctgatt gatccacctc ccggagaagt
     1501 catggaaggt gtactgacat cgggagcaac gatacctctc atcaatcagt ttgtacagct
     1561 tacggcagtt gcagcgaggg caaaattgat gcccattttt aaggcatttg ctcgacagat
     1621 accttcgaaa ttttctctcg ctgctgatga atcataataa cgggttagct ccataaggca
     1681 cccttagcat aggtgccaat atgcgatcca ttaccaagat ttatacttcc ataccaaata
     1741 attttcctgt ccaagactgc aaatttttga tggatttttg atttgtatat caaataaatt
     1801 tcccagaggt gttttacttt catttgcctg catcttcgcc tcccttgcga gttgtcaact
     1861 gattctgggt atcatcagta agaatccgct ctagccccta tgccattctg acctctacga
     1921 aatgttcccg tctgtcatca aacagcggaa caatgttgtc gtaccgcacg accccgtcga
     1981 cggtaatgct cgtccggtct tcgcccgcgc gcccttgcag cacactgatg tggtaaacgg
     2041 tttccctgta ccggtaatgc atcttgaagg ctttccaatc cgaaggaagg ctgggcgaga
     2101 gatgaagtct gtccacgtcc agccggatcc ccaggagcga ttccaggata agccggtaca
     2161 tccagccggc agcccccgtg taccacgtcc aaccgccccg gcccgtgtgg ggcgggcgcg
     2221 catacacgtc agccgccatg acatagggtt ccaccttgta cgtctcgatc tcttcggccg
     2281 atcgcccgtg attcacgggg ttgatcatgg ccaggagttc ccaggcatgc cgcccgtcgt
     2341 ccattgcggc aaaggccatg gccgcccaga tcgccccgtg ggtgtactgc ccgccgtttt
     2401 cccggacccc gggaacgtat cccttgatat agccgggatt tgaatcggac ttgtcgaaag
     2461 gcgggtccag cagttggacg agtctatcgt cccggcgcac aaggtgtttt ccaacggcct
     2521 ccatggccgt gctagcgcgc ccggcatcgc cggcgcccga gagaacggac cagctctgga
     2581 caatggaatc gatccggcat tcggtattgc ctgctgaacc gagcaacgag ccgtcgtcga
     2641 aataagcgcg caggtaccac cccccgtccc agccatgctg ctcgatattc cggcgcagtt
     2701 cggccaccgc ctgccggcat tgttcggcaa caggccggtc gccgcggcgg agcgcaatcc
     2761 cggagaattg catgaggacc tcacagagga aaaaccccag ccagacgctc tcgcctttgc
     2821 cgtcctttcc caccatgttc atgccgtcgt tccagtcgcc ggaaccgatg agcggcaggc
     2881 cgtgttcgcc tgtgcgctgg atgcccctcc tgacggcccg cacacagtgg tcgtacagac
     2941 tggccgtttc ctctgatgtg tggggcaggt cgtaataggc gtcttcctcg gggttgaccg
     3001 ggcggccttt gaggaagcgg accggctcat ccaatacccc gttatccccg gtggccagca
     3061 catagcggca cgcagccagg ggcagccaga ggaaatcgtc ggaacaatgc gtgcgcacgc
     3121 cccgtcctgc gtgcggatgc caccagtgct ggacatctcc ttccgcaaac tggcgggatg
     3181 cagcgagcag caggtgctca cgcatgagtc gcggctccgc gtggacaagc gccatgacgt
     3241 cctgcaactg gtcgcggaaa ccgaaggcgc ctcccgattg gtagtatccg ctccgcgccc
     3301 agaggcggca tgcgatggtt tggtaaacga gccagccgtt ggcaaggacg ttgatggacg
     3361 ggtcgggtgt ttccacctga actgcaccga gggtatgatt ccagtattgc caaatcgcct
     3421 ccaactcggc ccgcgccgcg ccggcgcctc caaaacggcg gatcaggccg cgggcgtcat
     3481 ccgcatcccg gcctgcgccc aaggtgaaga cgatctgacg ctcttccccg ccggcgagtt
     3541 cgaaggccac ctgcatggct ccgcagggat ccagggcggc ccccgtcttt ccggagagcc
     3601 gtgcacgggt catggcggcg ggactgcggg gggtgccgtt gcgcccgagg aattcggtcc
     3661 ggtccccgct tacggtgcgg gtcccgtcat ccacgttgaa aaatgcgacc cggtcgccga
     3721 attccgtatg gtaggggttg cgcgccagga gggcgccgct ttgcggatca acctcggtga
     3781 tcacgtgcag catcgttttc ggccgcaagt ctcccagcac ccactccgcg tacccggtca
     3841 cgtcgagcca ccgcggctga tccgtccggt tgcgcacctt caagaccgt
//
LOCUS       DAOPBQ010000370         3880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_19064_length_3880_cov_3.373595, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000370 DAOPBQ010000000
VERSION     DAOPBQ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19064_length_3880_cov_3.373595"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1834
                     /locus_tag="PKW60_11345"
     CDS             <1..1834
                     /locus_tag="PKW60_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00072.1"
                     /translation="PMREAVEQRIRQEGLDHTLRIRRERPENLGWVALEPSGAPAAPD
                     FAPCFASEAFCDSLLERYLQEMDRFRFRNIEVSGSPIVECHAAGHGHAPGVLSLMSAF
                     QGLVEVVASLKEGYAHVTCMRPYVSYGAGLARLCDGLAVMAEEHPLPLPDIHVGRLFA
                     DMQRLYFRRAHSFLIPRSKLSNSVGIAPEAAPNAPYPGAEHYPWYLYHDAAGWRYALI
                     SAIATAPRHRIHALPQDLPEADKAFALKWLTWERRNLVERPHVEEILGEPGIDAVDGY
                     SYGTAQGAVVFLFNTRYDPQELRLRLNLKHDLEYIVRELYPREYNYLGPNDGLFARNS
                     EFSCELAPKEARIIEVVRRSPAAAHRKRPEVFGAPARVEHGLVIIQGRPGASVDIVVR
                     REDQFTRRTVTFKGDPIPSRINEWAITRCLYEDGKDALPRGGFQGVPCGHGADIRRDA
                     WLCARICLPEDIRAHIDTQPFTLDRPCWASPKRLFFVIRFEIDDDFDPIRTASGVPGI
                     PEGYAARLPRKCGIDLAGRNYGLRAWLNGEPREVYPALASWQGYTPNPDPVVAYFFEA
                     GSKLKFGADNQVVLFASHFDPAAFKGISIEHLPDLYREEAVSGA"
     gene            1855..2985
                     /locus_tag="PKW60_11350"
     CDS             1855..2985
                     /locus_tag="PKW60_11350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK00073.1"
                     /translation="MITDPHLRGGGQVRYVANLAAELGRLGHRITIGCRRASVLVEHA
                     NAAGCAANQRFTFRGGLRPASWLTDLRETRRVIIEDKPDILHVNGSQDHWALAGANRL
                     MGRPVCVVRTRHNTYPVHDLWPNRMLNLQWTDFQIVVCEMVRRSLEKQASFDPARMCS
                     IHNGVDAKEFHPDPKLRAEARAEFGYRDDDLVCGIAARLVPAKGHVYLFQAAAMLLRD
                     FPALRLLVLGRGDLEPELAQLARDLGIAEIVHFAGFRDDIARCTQAFDIGVQPSVDCD
                     TSSFSLKEQMAEEIPVVCSDYGGLTEIVTDGVEGFVVPTGEAEPLALAMKTLLADPAQ
                     RRTMGSAGRQRVLQEFTLEVFARRTAEAYYQALEIHGAHSAS"
     gene            2966..>3880
                     /locus_tag="PKW60_11355"
     CDS             2966..>3880
                     /locus_tag="PKW60_11355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK00074.1"
                     /translation="MRILHLDEQRGWRGGEQQASYLIQGLARRGHFVAIAGRPEGEFL
                     RRDHGLENLERVACPFRGELDPVTVWRLAQAVKRLDIDILHAHASHAHSLACLARACA
                     GRGKVIVTRRVDFVPKPGFLNRKKYRLPDHIACVSQCIAGILREFGVPASKLTVINSA
                     QDPKRFGVVPFSRAELGLPEEVPLFGNVGAFVGHKDHATLIRAFKQAREALLEAHLAL
                     VGDGELRGELEAAIEAEGLSGSVTLLGYRKDVPRILPVFDAFVLSSRQEGFGGVCAEA
                     MFAGVPVVSTDAGGMIETVKPGITGLMVA"
BASE COUNT          714 a         1173 c         1220 g          773 t
ORIGIN      
        1 tcccatgcgg gaagccgtcg aacagcgtat ccggcaggaa gggctggatc acaccctgcg
       61 catccggcgc gaacgccctg aaaatctggg ctgggtcgcc ctggagcctt ccggcgcgcc
      121 cgccgcgccg gattttgcgc cctgtttcgc ctcggaggcg ttctgcgatt cacttcttga
      181 acgctacctc caggaaatgg accgcttccg cttccggaac atcgaggtca gcggaagccc
      241 tattgtggaa tgccatgctg ccgggcacgg gcatgccccg ggcgtgttgt cgctcatgag
      301 cgcgtttcag gggttggttg aggtcgtggc ctcgctgaag gaagggtacg cgcatgtgac
      361 ttgtatgcgc ccctacgtca gctatggcgc cggccttgcc cggctctgtg acggactggc
      421 cgtcatggcg gaagagcacc ccttacccct tcccgatatc catgtgggcc gcctgttcgc
      481 tgacatgcag cgtctgtatt tccgccgggc ccacagcttt ctgatccccc gaagcaagct
      541 gtccaattcg gttggaattg cgccggaagc agccccgaac gctccgtacc ctggcgcgga
      601 acattatccc tggtacctgt accacgacgc agcggggtgg cggtacgcgc tcatttctgc
      661 gattgccacg gcgccccgtc accgcataca tgccttgccc caggatctcc ctgaagccga
      721 caaggcgttc gcgctcaaat ggctgacgtg ggagcgcagg aacctggtgg agcgcccgca
      781 tgtggaggag atcctgggtg agccgggaat agacgctgtt gatggctaca gctacggcac
      841 agcccagggg gcggtggtgt ttcttttcaa cacccgctac gacccgcagg aactgcgctt
      901 gcggctcaac ctcaaacacg acctcgagta catcgtacga gaattgtatc ctcgcgaata
      961 caattatctt gggcccaatg acggcctttt tgcgcgcaac agcgaattct cctgtgaact
     1021 ggctcctaaa gaagcgcgaa tcatcgaagt ggttcgacgt tctccggcgg ccgcgcatcg
     1081 gaagcggccg gaggtgttcg gcgcgcctgc ccgggtggaa cacgggctcg tcatcatcca
     1141 gggccggccg ggggcgtctg tggacatcgt ggtccgccgg gaagatcaat tcacgcgcag
     1201 aaccgttacg ttcaagggag atccaattcc atcccgcatc aacgaatggg caatcactcg
     1261 ctgcctttat gaagatggca aagatgcgtt gccacgcggt ggatttcagg gggtcccgtg
     1321 cgggcacggt gcggatatcc ggcgcgacgc ctggctctgt gccaggatct gccttcctga
     1381 ggacatccgg gcacacatcg acacgcagcc gttcaccctg gatcgccctt gctgggctag
     1441 tccgaaacga ctcttcttcg tgatccgctt tgaaatcgac gacgatttcg acccgattcg
     1501 cacggcgtcc ggcgtgccgg ggattccaga aggctatgcg gctcgcctgc cgcgaaaatg
     1561 cggcatcgac ctcgccggaa ggaactacgg acttcgcgcc tggctcaacg gggaaccccg
     1621 cgaggtctat cccgccctcg cgtcctggca gggctacacc ccaaacccag atcctgtggt
     1681 agcctatttt tttgaggcgg gaagcaagct caaatttgga gcagacaacc aggtggtctt
     1741 gttcgcctcg cattttgatc cggctgcctt caaaggcatc agcatcgaac acttgccgga
     1801 cctgtaccgg gaagaagcgg tttccggggc gtgacggagc gcccgctgca tatcttgatc
     1861 actgacccgc atctgcgagg cggaggccag gtccgctatg tcgccaatct ggccgctgag
     1921 ctcggccgtc tgggccatcg catcaccatc ggatgcagac gcgcctccgt gctggttgag
     1981 catgcgaatg ccgcgggctg tgcggccaac cagcgcttta cgtttcgtgg cgggctgcga
     2041 cccgccagct ggctgacgga tctccgggaa acccgccgcg tcatcatcga ggacaagccc
     2101 gacatccttc acgtcaacgg ctctcaggat cattgggcgc tggccggggc aaaccgcctg
     2161 atgggaaggc cggtgtgcgt cgtgcggacc cgccacaaca cctatcccgt tcacgacctc
     2221 tggcccaacc gcatgctcaa cctgcagtgg acggactttc agatcgttgt gtgcgagatg
     2281 gtgcgccgct cgctggaaaa gcaggcgtcg ttcgaccccg cccggatgtg ctcaatccac
     2341 aatggcgttg atgcgaaaga attccaccct gaccccaaat tgcgtgctga ggcgcgcgct
     2401 gaattcggct accgggatga tgatctggtg tgcggcattg ccgcgcgcct ggttccagct
     2461 aagggtcacg tttacttgtt ccaggcggcg gccatgctgc tgcgcgattt cccggcgctg
     2521 cgcctcctgg tgttgggccg gggcgatctg gagcctgagc tggcccagct tgcccgcgac
     2581 ctgggcattg ccgaaatcgt gcacttcgcg ggatttcgtg acgatatcgc ccgctgcact
     2641 caggccttcg acatcggcgt acaaccttcc gttgactgcg atacgtcatc tttcagtctg
     2701 aaagagcaaa tggcggaaga aatacctgtg gtttgttccg attacggagg attgaccgag
     2761 atcgtgaccg acggagtcga aggatttgtt gttccgacag gtgaagcgga gcccctggca
     2821 ttggcaatga agacgctgct ggcagatccc gctcaacgcc gtacaatggg gagcgcggga
     2881 cgccagcgag tgcttcaaga atttacgctg gaggtgtttg cgcggcgtac cgctgaggca
     2941 tattaccagg cattggagat tcacggtgcg cattctgcat cttgacgagc aacgcggctg
     3001 gcgcggggga gagcagcagg ccagttatct gatccagggt ctggcccggc gcggacactt
     3061 tgtcgcgatc gcgggccgcc cagagggtga attcctgcgt cgagaccacg gcttggaaaa
     3121 cctcgagcgt gtggcgtgcc cgttccgcgg agaacttgat ccggtgacgg tctggcgcct
     3181 cgcacaagcg gttaagcgcc tcgacatcga catccttcat gcccacgcga gccacgccca
     3241 cagcctggca tgtctcgcgc gcgcgtgcgc gggccgcggc aaagtcatcg tgacccggcg
     3301 tgtggacttt gtgcccaaac ccggtttttt gaaccggaaa aaatacagat tacccgacca
     3361 catcgcctgc gtgtcgcagt gcattgccgg gatcctgcgt gaattcggcg tgccggcatc
     3421 aaaactgacc gtcatcaaca gtgcccagga tccgaaacgc tttggcgtcg tgcctttcag
     3481 ccgcgccgaa ttgggtcttc ctgaagaagt gccgttgttc ggcaatgtcg gggcgtttgt
     3541 tggccacaaa gaccacgcga cgcttatccg ggcattcaag caggccaggg aagccttact
     3601 cgaggcccac ctcgccctgg tgggagatgg agaactgcgc ggagaactcg aggcagcgat
     3661 agaggcggag ggactctccg ggagcgtgac gctgctgggg tacaggaagg acgtgccccg
     3721 gatattgccg gtattcgatg cgtttgtgct gtcgagcaga caggaaggat tcggcggggt
     3781 atgcgcggag gccatgtttg ccggggttcc ggtggtttcg actgatgccg gcgggatgat
     3841 cgagacagta aaacccggca tcaccggact gatggtggcc
//
LOCUS       DAOPBQ010000371         3877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_19084_length_3877_cov_3.783359, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000371 DAOPBQ010000000
VERSION     DAOPBQ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19084_length_3877_cov_3.783359"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1561)
                     /gene="gcvPB"
                     /locus_tag="PKW60_11360"
     CDS             complement(128..1561)
                     /gene="gcvPB"
                     /locus_tag="PKW60_11360"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPK00075.1"
                     /translation="MELIFEKSRPGCRAIAFDPLDVPEAALPPELCRAEPAALPEVSE
                     LDVVRHFSRLSQRNFGVDTHFYPLGSCTMKYNPKMAEAAAAHPGFLGLHPHLASAPAT
                     WPLCQGAFELIFELERLLAGIAGMRQVSLQPIAGAHGELTGTLMIAAYHRDKGNTKDT
                     ILIPDSAHGTNPASAAIAGFKVVEVPSLPDGTLNLNAFKKALNESVAAVMLTIPNTHG
                     LFEPRVLEIAQLAHEMDALMYNDGANLNAIIGECRPGDLGFDVMHFNLHKTFATPHGM
                     GGPGAGPVGVGERLEAFLPGPRVVRDGEGFGLASPPKSIGNISAFFGNFLVAVRAYAY
                     ILKLGGEGLREVAHHAVLNANYVYARLKDLFEPAFDGPFMHECVFSATPLAKYGARAL
                     DVAKALLDYGFHAPTVYFPLTVKESLMIEPTETESKDTLDAFCSAMAEIASLAASEPE
                     RLHAAPVSLPVGRLDEVRAARELNCRV"
     gene            complement(1581..2870)
                     /gene="gcvPA"
                     /locus_tag="PKW60_11365"
     CDS             complement(1581..2870)
                     /gene="gcvPA"
                     /locus_tag="PKW60_11365"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPK00076.1"
                     /translation="MSFVSTTPAEQAEMLKTIGLTSTEEFFEHIPETLRVTSWDLPPG
                     QSEMAVRARLAQLASRGDGARVCFLGGGYYDHFIPAAVDALASRGEFLTAYTPYQPEA
                     AQGTLQSIYEYQSAICRLTDMDFANASLYDGGTAVFEAATMAIRLTRRRRIVCHGSLN
                     PRYRAILATHTANLEVELADGDDPAGAACVIVQNPGFLGDVHDFTALAGRCHEAGALL
                     VLSFYPLSLGLLKTPGAMGADIAVAEGQSLGVPLGFGGPYLGILATRKEHLRRMPGRL
                     AAATEDAQGRRGFVLTLQAREQHIRREKAMSNICSNEALIALRALIYLCLLGRDGLRE
                     AATQCHAKAEYLKQQLGFARLLNSGSTFNEFAVRLPVKAQDACLALAKQGYLAGLPLS
                     AVGKGEETDLLIAVTEQRTRAQLDVFAGALASACRQA"
     gene            complement(2867..3259)
                     /gene="gcvH"
                     /locus_tag="PKW60_11370"
     CDS             complement(2867..3259)
                     /gene="gcvH"
                     /locus_tag="PKW60_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPK00077.1"
                     /translation="MDFPEGLKYTHEHEWIRDEGGDIYTVGITEHAAEQLGDVTYVEL
                     PEPGMVLDQGQAAGAVESVKAASDIYAPISGHVSEVNQELDRRPELVNASPYNDGWFF
                     KIAEGNLREYDKLMDCKEYARFVEDQGV"
     gene            complement(3273..>3877)
                     /locus_tag="PKW60_11375"
     CDS             complement(3273..>3877)
                     /locus_tag="PKW60_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HPK00078.1"
                     /translation="LTAAAALSYFGLTQAEWSGYPVLLSRTGYTGELGYELYLPNAAA
                     VPLWRRLLDHPAVAPAGLGARDTLRLEMGYLLSGQDIDGSRTPLEARQDAFIDWNKPF
                     TGREALEKQRDAGGYSVLTPIWTDSRSAPRHGFDVYRGDETVGVVTSGTYGPSVERGI
                     GLAYLSPELAEPGVTLTAGPRRIAIEVTEIPFYGAGTCRN"
BASE COUNT          710 a         1189 c         1274 g          704 t
ORIGIN      
        1 ggacactccc agaatcgcag gctttccccc gccctgcccc gctcggcgga atccagcagg
       61 acttcgtcca gggccaggtt cgccgcgggt tcgtgaaatg agaggtccag cacacgcaca
      121 gagccgccta taccctgcaa ttgagctcgc gagcggcgcg cacttcgtcg aggcggccga
      181 caggaaggct gacgggggcg gcatggaggc gctccggctc gctggccgcc agactcgcga
      241 tttcggccat ggcgctgcaa aacgcgtcga gcgtgtcttt ggattcggtt tccgtgggct
      301 cgatcatcag gctttccttg acggtaagcg gaaaatacac cgtgggagca tgaaaaccgt
      361 agtcgagcag ggccttggcg acatcgagcg cccgcgcgcc gtattttgcc agaggcgtag
      421 cggaaaagac gcattcgtgc atgaatgggc cgtcaaacgc gggttcgaag aggtctttca
      481 ggcgggcgta cacataattc gcgttgagca cggcgtgatg ggccacctcg cgcaggcctt
      541 ccccgcccag cttcaggatg taggcgtagg cgcgtaccgc gaccaggaaa ttgccaaaaa
      601 acgcggagat gttcccgatc gattttggcg ggctggccag gccaaacccc tctccatcgc
      661 gcactacgcg cgggccgggc agaaacgcct ccaggcgctc gccgactcca acgggccccg
      721 cgccgggtcc gcccatgcca tgcggcgtgg cgaaggtctt gtgcagattg aagtgcatga
      781 cgtcgaagcc gaggtcgcca gggcggcatt ctccgataat ggcgttaagg ttggccccgt
      841 cgttgtacat gagggcgtcc atctcatggg cgagctgcgc gatttcgaga acgcgtggct
      901 caaacaggcc atgggtgttg gggatggtca acatgacggc cgcaacggat tcgttgaggg
      961 cctttttgaa tgcgttgaga ttcagggtgc cgtcggggag cgagggcacc tcgaccacct
     1021 tgaacccggc gatggccgcg ctggcgggat tcgtgccgtg ggcagaatcg gggatcagga
     1081 tggtgtcttt ggtattgcct ttgtcgcgat ggtaggcagc gatcatgagg gttccggtca
     1141 attcaccgtg ggctccggcg attggctgga ggctgacctg gcgcatgccc gcgatcccgg
     1201 ccagcaagcg ctcgagctca aagatcagct cgaaggctcc ctggcacaga ggccacgtag
     1261 ccggcgcgga agccaggtgg ggatgcagcc ccagaaaccc cgggtgcgcg gcggctgcct
     1321 cggccatttt cggattgtat ttcatagtgc aggagccgag cggataaaaa tgggtgtcga
     1381 cgccgaagtt gcgctgcgac agacgcgaga agtgccgcac gacgtccagt tcgctgacct
     1441 cgggaagggc tgcaggctcc gcgcggcaca gttcgggcgg aagcgccgct tccgggacat
     1501 ccagcgggtc gaacgcgatg gcccggcaac cgggtctcga tttttcaaaa atcagttcca
     1561 tactctggct ccatatcggg ctatgcctgg cggcaggcgc tggccaatgc gccggcaaac
     1621 acgtcgagct gggcgcgcgt acgctgttcc gtaacggcaa tgagcaggtc tgtctcttcc
     1681 ccttttccaa cggcgctgag aggcagcccg gcaagataac cctgtttcgc aagcgccagg
     1741 caggcgtcct gggctttgac ggggaggcgg accgcgaact cgttgaacgt ggacccggaa
     1801 ttgagcaacc gggcgaatcc gagctgttgt ttgaggtatt ccgccttggc atggcactgg
     1861 gtggccgctt cacgcaggcc gtccctgccc agcaggcaca ggtaaatcag cgcgcgaagg
     1921 gcgatgagcg cctcattcga acagatgttg gacatggctt tctcgcggcg gatgtgctgt
     1981 tcgcgggcct gcagggtcag gacgaatccg cgccggccct gcgcatcttc ggtagccgca
     2041 gccagccggc cgggcatgcg gcgcaggtgt tccttgcggg tggccagaat gcccaggtag
     2101 gggccgccga aacccagcgg cacgcccaga ctctgtcctt ccgctaccgc gatatccgca
     2161 cccatggcgc ccggcgtttt cagaaggccc agcgacaagg ggtagaacga gagaacaagc
     2221 agcgcgcccg cctcgtggca gcgcccggcc agcgccgtaa aatcgtggac atctcccagg
     2281 aaacccgggt tctgaacaat gacgcaggcc gcaccggccg gatcgtcgcc gtcggcgagt
     2341 tcgacctcga gattggcggt gtgcgtggcc agaatcgcgc ggtagcgcgg gttgagggat
     2401 ccgtgacaga cgatgcgccg ccggcgcgtg agccggatcg ccatcgtagc ggcttcgaaa
     2461 accgccgtgc caccgtcgta gagcgacgcg ttggcaaaat ccatatcggt cagccggcag
     2521 atggcggact ggtattcgta gatactctgc agggtgccct gggcggcttc gggctggtac
     2581 ggtgtgtagg cggtgagaaa ttctccgcgg gacgcgagcg catcgacggc ggccgggatg
     2641 aagtggtcgt aatagccgcc gccgagaaag cagacgcgcg ccccatcacc gcggctggcc
     2701 agctgggcca gccgcgcacg cacggccatc tccgattgac ccgggggcaa gtcccaggaa
     2761 gtgacgcgca gggtttcggg gatgtgctca aaaaactcct cggtgctcgt aagcccgatg
     2821 gttttaagca tttcagcctg ttcggccggc gtggtggaga cgaagctcat acaccctggt
     2881 cctccacaaa gcgggcgtac tccttgcagt ccataagctt gtcgtattcc cgcagattgc
     2941 cctcggcgat tttgaaaaac cagccgtcat tgtaggggct ggcgttcacg agttccgggc
     3001 ggcggtccag ctcctggttg acctcagaaa cgtggccgct gatgggggca tagatgtcgc
     3061 tggcagcctt gactgattcc acggcgcccg cggcctggcc ctgatccagc accatgccgg
     3121 gctcgggcaa ttcgacgtag gtcacatcgc ccaattgctc cgcggcatgt tcggtgatgc
     3181 ccaccgtata gatgtcgccg ccttcgtctc ggatccactc atgttcgtgc gtgtacttga
     3241 gtccttcggg gaaatccata gtcgctctct ccttaattcc tgcatgttcc cgccccgtaa
     3301 aaggggattt ccgtgacttc tatggcaatt cggcgggggc ccgcggtcag tgtgacgccg
     3361 ggttctgcca gttcaggcga cagataggcc agtccaatgc cgcgctcgac cgaaggcccg
     3421 taggttccac tggtgacaac tcccacggtc tcatcgcccc ggtatacgtc aaatccgtga
     3481 cgcggcgcgc tgcgcgaatc cgtccagatc ggtgtaagaa cagagtatcc accggcgtcc
     3541 cgttgctttt ccagcgcctc ccggcccgta aacggtttgt tccagtcgat gaacgcatcc
     3601 tgacgcgctt ccagcggcgt gcgcgaccca tcgatatcct ggcccgagag caggtatccc
     3661 atctccagcc ggagggtatc gcgcgcaccg aggcccgcgg gcgccacggc gggatggtcg
     3721 aggagcctcc gccagagcgg aacggcggcg gcgttgggca gatacagctc gtaacccagt
     3781 tcgccggtat atcccgttct cgagagaagc accggatacc cggaccattc cgcctgtgtc
     3841 agcccgaaat aactcaacgc cgcggcggca gtcagcc
//
LOCUS       DAOPBQ010000372         3811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_19491_length_3811_cov_3.787274, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000372 DAOPBQ010000000
VERSION     DAOPBQ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19491_length_3811_cov_3.787274"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="PKW60_11380"
     CDS             complement(<1..1162)
                     /locus_tag="PKW60_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00079.1"
                     /translation="MMFSGDPQPGDRNWGADDGQINAMRARLAEAEKNLGNIELIIGQ
                     VHTPEETQALLESAGPDAPVLAINVMNFALTRVVAPVLESGRPMLVFSLPASGHDWMY
                     APRWHRAGQPVTLMTTSNYDELERALRLLRVPSMMRQSRILLFPPARGTRAACSPEEI
                     KARLGAEVIAVEDKLFEDLIANADEAAVRAEYDAWTQGAKEIIEPTREDIEKAARVSV
                     ALDRLVEQEQADALAIGTCMDWLSRGFPCLGFTRLRDRGIPAACEGDMDSLLTMLMFQ
                     YALDQPGFQGNATFDTSRNVLWTAHCTAPLKMDGAEGKAAPYLLRGHSEVGGSGCVPE
                     VQYRVGETITRTKFVNLSTLLASTGKIVEVPERSVHGCRTQIVTEVRDAAKMA"
     gene            complement(1565..2938)
                     /locus_tag="PKW60_11385"
     CDS             complement(1565..2938)
                     /locus_tag="PKW60_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPK00080.1"
                     /translation="MNQPNGRIQRFAAALGPVLMYGLLAAGFVLAGYQVWRTIRNEAP
                     DRKITLDFLWPTYTPQKVRYGEYLAQEYMRENPDVHINLILTPDPYRKLQVMIAGRTT
                     PDVAWMGIGWQQFADAFLPLDERVAADPAMQAQNYFPALWESVQWRGRMLALPSSGQV
                     GVVYYNKDLFDEAGLDYPADDWTWDDMQRMARALTRDFDGDGIVDQYGLQLGQVSLAP
                     FLLYDGQIADPEWQTARVDSPVSRALLERYQALIYEDKVMPTPTTSLELGMLPMFEAG
                     RVAMHAATGYAIESFRKVQFDWDVVRLPAFEFEGQRYQATGLWQEEFSILWDTDEPDA
                     AWDFARWCAGHDMVRWAALNGHIVPGRMDVALSDEYQHSGRRPANMRAFTDSLAFAVP
                     INPHPWSRRIAIEFEPVLERYHVGTEGRRITAAETLPLLQPILQNILDEYNTEHNQPR
                     LNGRVHQ"
     gene            complement(2935..>3811)
                     /locus_tag="PKW60_11390"
     CDS             complement(2935..>3811)
                     /locus_tag="PKW60_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPK00081.1"
                     /translation="IPQGRAETVDVGIKSLASRRRLTTVYVPDGSFEAAIEAWRVRPL
                     SGEDETAEAFWAPFDSVRRRLAPEWGNYREALEKMRFWVCLANTLAITFLGTAGTILS
                     CSLVAYGFARFRFPGNSALFLVLLSSIMLPSVVTMIPVYLLFRELRWIDTLLPLFAPA
                     WLSVNAFAVFLFRQYYMTLPFDLDDAAQIDGCSALGTYWRVLLPLSRPVMATVGIFCF
                     TQYWLDFMGPLIYLNTSDKFTLALGLYTFKGAYTTQWHYLMAATLAVSLPCIVLFFAG
                     QRFFVRGVVMSGLKA"
BASE COUNT          739 a         1163 c         1200 g          709 t
ORIGIN      
        1 cggccatctt tgccgcatcg cggacctcgg tcacgatctg ggtgcggcaa ccgtgcacgg
       61 agcgttcggg gacttcgaca atctttcccg tcgaggcgag cagcgtcgac aggttgacga
      121 atttggttcg ggtgatggtt tcgcccacac ggtactgtac ttcggggacg caaccgcttc
      181 ctcccacctc ggaatgcccg cgcagcagat acggcgcagc cttgccctcc gcgccgtcca
      241 ttttcaacgg agcggtgcag tgggcggtcc acagcacgtt gcgcgaggta tcgaatgtgg
      301 cgttaccctg gaagccgggc tggtccaggg catactggaa catgagcatg gtcagcagcg
      361 aatccatgtc gccttcgcag gcggcgggga taccgcggtc gcgcaggcgt gtgaaaccca
      421 gacaggggaa accgcgcgaa agccaatcca tgcaggtgcc gatggccagg gcgtcggcct
      481 gttcctgctc gacgaggcga tccagcgcga cactgactcg cgcggctttt tcgatgtcct
      541 cgcgggtggg ttcgatgatc tctttggcgc cctgggtcca ggcgtcatac tcggcgcgca
      601 cggcggcttc gtccgcgttg gcaatcaaat cttcgaacag cttgtcttcg accgcgatga
      661 cctcggcgcc cagccgtgcc ttgatttctt ccggggagca ggcggcccgg gtgcctcgcg
      721 caggcggaaa cagcagaatg cggctctgcc gcatcatgga tggcacgcgc agcaggcgca
      781 gcgcgcgctc cagctcgtca taattgcttg tggtcatcaa cgttacaggc tgtccggcgc
      841 ggtgccatcg gggggcgtac atccagtcgt gcccgctggc cggcaacgaa aacaccagca
      901 tgggccggcc cgactcgagc accggggcga ccacgcgcgt caacgcgaaa ttcatcacgt
      961 tgatggccag tacaggggca tccggccccg cgctttccaa taacgcctgg gtttcttccg
     1021 gagtgtggac ttgtccgatg attaattcga tattgcccag gttcttctcg gcttcggcaa
     1081 gccgggcccg catggcgttg atctggccgt cgtcggcgcc ccagttacgg tcgcccggct
     1141 ggggatcgcc ggaaaacatc acgcaaatgc ggtttttccc acgcggagct gcgggtgcag
     1201 cttgtgcagt ccaggtatga gtccaggttg cgccggccag gagcaatccg ccggctgcgc
     1261 ccaatccgaa gaactccctt cgcgtacagt gctcacacat tgccgaatcc tttccctgtc
     1321 caggccgtgc cttgcgtttt tcgagcgttg gcgtcgtgca tgagtgcgcc gggtctcccg
     1381 gagaggcgct tatgccaaat ctcgagagcc agtgcgccgg caaccgcagt ccgggctgca
     1441 ctgcccgatc cgcgtgcgcc acgccagaat agtccaggct tctcatgcgt ttcaattgct
     1501 tccggccagg cagcgtgatc tttgagagca ggcgcagcag cgcggacatc atgctcgcgc
     1561 tgcgttattg atgaacacgg ccgtttaagc ggggttggtt atgttccgta ttgtattcgt
     1621 cgaggatgtt ctgaaggatc ggctgcaata gcgggagtgt ttccgcggcc gtgatgcgcc
     1681 ggccttcggt gccgacgtgg tagcgttcca gtacgggttc aaactcgatc gcgatgcgtc
     1741 ttgaccaggg atgtggattg atgggcacgg cgaatgccag tgaatcggtg aaggcccgca
     1801 tgttggccgg ccgccgcccc gaatgctggt attcgtcaga cagggcgacg tccatacggc
     1861 ccggcacgat atgtccgttt aacgcggccc agcgcaccat gtcgtgcccg gcgcaccagc
     1921 gcgcaaaatc ccaggccgcg tccggttcgt ctgtgtccca caaaatcgag aattcttcct
     1981 gccagagccc cgtggcctgg tagcgctgcc cctcaaactc gaatgcgggg aggcgcacga
     2041 catcccagtc gaactggact ttgcggaacg attcgatggc gtatcccgtg gcggcatgca
     2101 tggctacccg gccggcctca aacatgggca acatgccgag ctccagtgag gtggtcggcg
     2161 tgggcatcac cttgtcctcg tagatcagcg cctggtagcg ctccagcagt gcgcgggaca
     2221 cggggctgtc cacacgggct gtctgccatt cgggatcggc aatttgacca tcatagagca
     2281 aaaacggagc cagggacacc tgtcccagct gcaatccata ctggtcgacg atgccgtcgc
     2341 catcaaaatc acgggtcagg gcacgcgcca tgcgctgcat atcatcccag gtccagtcgt
     2401 cggcggggta gtcaagcccg gcctcgtcaa agaggtcttt gttgtagtag acgacgccca
     2461 cctggccgct ggacggcaga gccagcatcc ggccgcgcca ttgaacggat tcccacaggg
     2521 cggggaaata attctgagcc tgcatcgcgg gatccgcagc gacccgctcg tccagcggta
     2581 gaaaggcatc ggcaaactgc tgccacccaa tacccatcca ggcgacgtcg ggcgtggtgc
     2641 gtcccgcgat catcacctgc aatttgcggt aggggtcggg tgtcaggatc aggtttatgt
     2701 gaacatcggg attttctcgc atgtattcct gggcaaggta ttcgccatag cggacttttt
     2761 gcggcgtata ggtgggccac aggaaatcga gggtgatctt tcggtccggg gcttcgtttc
     2821 ggatggtgcg ccaaacctgg tagcccgcca aaacaaaacc cgcagcgaga agcccgtaca
     2881 tcagcacggg ccccaaagct gccgcaaagc gctggattct cccattgggc tggttcatgc
     2941 cttcaatccc gacatgacca cgccacgcac gaagaagcgc tgcccggcga aaaataagac
     3001 gatgcacggc agcgatacgg ccagcgtggc ggccatcaga taatgccatt gtgtggtgta
     3061 ggcgcccttg aacgtgtaca ggcccagggc aagcgtgaat ttgtcgctgg tgttcaggta
     3121 gatgagcggc cccatgaaat ccagccagta ctgcgtgaag cagaaaatac ccaccgtagc
     3181 catcaccggc cgcgacaagg gcagcagtac gcgccagtag gtgcccaacg cgctgcaacc
     3241 gtcgatctgc gctgcgtcgt cgaggtcgaa cggcagcgtc atgtaatact ggcgaaaaag
     3301 gaatacggca aacgcattca cggagagcca ggccggggcg aacagaggca gcagggtatc
     3361 gatccagcgc agctcgcgaa aaagcaggta gaccgggatc atcgtcacga cggacggcag
     3421 catgatggag gagagcagga cgagaaagag cgcgctgttg cccggaaagc gaaaccgggc
     3481 gaatccgtag gccaccagtg agcacgagag gatcgtcccc gccgtaccga ggaaggtgat
     3541 ggcgagggta ttggccaggc agacccagaa acgcattttt tcaagcgctt cccggtaatt
     3601 tccccattcc ggggccagcc gcctccgtac cgaatcgaag ggcgcccaga aggcttcagc
     3661 ggtctcgtcc tctcccgaga gcgggcgcac ccgccacgcc tcaattgccg cctcgaaaga
     3721 accgtccggg acgtagactg tcgtaaggcg gcgccgcgat gccaggctct tgatgcccac
     3781 gtccacagtt tccgcccggc cctggggtat c
//
LOCUS       DAOPBQ010000373         3810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_19495_length_3810_cov_3.678828, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000373 DAOPBQ010000000
VERSION     DAOPBQ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19495_length_3810_cov_3.678828"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PKW60_11395"
     CDS             complement(<1..577)
                     /locus_tag="PKW60_11395"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPK00082.1"
                     /translation="MTKRFISHLSVWIMLALALFAMGGRAAAAPAENAPQPVPPQEVF
                     PLPETRYTTPAFTEKILQSGGRVDFVFGSDRPFAQCHASTIAQAPSGELVCAWFGGTE
                     EKNPDVGIWYSRFADGAWSPPERAAKVNETAHWNPVLFTDPESGVYLFFKVGPEIPYW
                     QTYWMKSTDAGLTWSAPTELVRYSFASTANTW"
     gene            721..2010
                     /gene="hflX"
                     /locus_tag="PKW60_11400"
     CDS             721..2010
                     /gene="hflX"
                     /locus_tag="PKW60_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPK00083.1"
                     /translation="MTEGKNERLIELERPPVIETAVLVRLILPADGVREIDDSLEEMR
                     RLAWTAGADVAATLVQRRAKPCPGTLIGRGKLEALRAACEEHEAGLVIFDCDLSPSQG
                     ANIADALGVKVVDRTQLILDIFALRAQSNEGKQQVELAQLEYLLPRLTGRGSAMMRQQ
                     GGIGVRGPGEQKLETDRRIVRERIRRLKAGLESTRKSRRIQRKRRIEGGIGTVALVGY
                     TNAGKSSLLNALTQAGAFVEDKLFATLDTTTRRRELPSGREVTFTDTVGFIRNLPHSL
                     VAAFRATLEEVNEADVLLMVADASHPAAEDHMKAVRAVLDEIEVGEKPFVLALNKMDL
                     ATPMQASELETRFGRGVRVSARTGEGIEELLHAIDANLMPQRSRVHLRIPQDQGGIVA
                     RVHDFGRVIQQNYEDNVVVLEAEVDVALRQQLADYIV"
     gene            2190..2522
                     /locus_tag="PKW60_11405"
     CDS             2190..2522
                     /locus_tag="PKW60_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00084.1"
                     /translation="MMRPEDDHSHVEPASATARDQRAPLTFWIFPAALALGVLGDLLL
                     RAWPYGLNAALWAVATLACAAGLLHWRERRLTPWTTVFLAASAAFALCLAWRDSAYLK
                     SLALSVCW"
     gene            complement(2508..3668)
                     /locus_tag="PKW60_11410"
     CDS             complement(2508..3668)
                     /locus_tag="PKW60_11410"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HPK00085.1"
                     /translation="MEIYGKKYSTKELRTRVGNMDQIAGIRTVQLDDGNERPTRAAII
                     HTGAGLELTVLLDRCLDISAASFNGKAMGWRSTTGDVAPQYFEAEGLRWLRSYFGGLV
                     TTCGLMNVGAPDEASALLGTGLHGRIGNTPAKNIKITQEWQGNDYVLSVSGTMRETVV
                     FGENLTLTRTVSTRLGEKRFWIHDSVTNDGFKPAKYMLLYHCNIGWPAVDQGAEIIAP
                     SRYAAPRDAEAENGKQNWYKLDPPTRGYREKVYYHDMVGDARSNVTAAIVNSGFKGKG
                     EGFGVYLKYNKKELPRFVEWKQMGEQDYVVGFEPCNCGVEGRAVDEKQGLLHSLRAGQ
                     SQEVHLEFGALTDAKEVAALRQACGKVKTEIVDSYKRFVKKPRYPSLRSPAY"
BASE COUNT          701 a         1110 c         1227 g          772 t
ORIGIN      
        1 cccaggtgtt ggcggtggag gcaaagctgt aacggaccag ttcggtgggc gcgctccacg
       61 tcaggcccgc atcggtggat ttcatccaat aggtctgcca atagggaatt tcgggcccga
      121 ctttgaagaa gaggtatacg ccggactcgg gatcggtgaa cagcaccggg ttccaatggg
      181 cggtttcatt tactttggcg gcgcgctccg gaggcgacca cgcgccgtcg gcaaaacggg
      241 aataccagat gcccacatcg ggattttttt cttccgtgcc gccgaaccac gcgcagacca
      301 gctcgcccga gggcgcctgg gcaatggtgg aggcatggca ttgcgcgaag gggcgatccg
      361 acccgaagac gaaatcgacc cggcctccgc tttgaaggat tttttcggtg aacgcgggcg
      421 tggtgtagcg tgtttccggc aagggaaata cctcctgcgg cggtacgggt tgaggcgcat
      481 tttcggccgg tgcagccgcg gcacggccac ccatggcgaa cagggccagc gccagcatga
      541 tccatacgga tagatgagag ataaaacgct ttgtcacgat gcttgcgatc ctttcgttgt
      601 tgtccctgtg caggtcccga ttttccgtgt atgcatagac taggagcccg cacggactaa
      661 ttcaacctta ctttcgtgat atagtagtgc gaaaggcctg aaaacacgaa agacgagggt
      721 atgaccgaag gtaaaaatga acgattaatc gagctcgaac ggcctcctgt gatcgagacc
      781 gccgtcctgg tgcgcctgat cctgccggca gacggcgtcc gggaaatcga cgattcgctc
      841 gaggagatgc ggcggcttgc ctggactgcc ggggccgatg tcgccgcgac gctcgtgcag
      901 cgccgggcca aaccgtgccc gggcacgctg atcggccggg ggaaactcga ggccctgcgg
      961 gcggcgtgtg aagaacacga ggccggcctg gtcatttttg actgtgatct gtcccccagc
     1021 cagggcgcca acatcgccga cgcgctcggc gtcaaggtgg tggaccgcac gcagttgatt
     1081 cttgatattt tcgcgttgcg cgcccagtcc aacgagggta aacagcaggt tgaactggcc
     1141 cagctcgagt atctcctgcc gcgcctgacc gggcgcggct cggccatgat gcgccagcag
     1201 ggcggcatcg gggtgcgcgg cccaggtgag cagaagctcg agacggaccg gcgcatcgtg
     1261 cgcgaacgca tccgccgcct gaaagcaggg ctcgagagca cgcgaaaaag ccgacgcatt
     1321 cagcggaaga ggcggatcga gggggggatc ggcacggtcg cgctggtggg ctacaccaac
     1381 gcgggcaagt cgtcgctgct taacgccctg acccaggccg gcgcgttcgt tgaggacaag
     1441 cttttcgcga cgctcgatac cacgacgcgg cggcgcgagc ttcccagcgg gcgcgaggtg
     1501 acgtttaccg acaccgtagg gttcatacga aacctgccgc actcgctggt tgcggcattc
     1561 cgcgccacgc tcgaagaagt gaacgaagcg gatgtgctgc tcatggtcgc tgatgcgtcc
     1621 caccccgcgg cagaagatca catgaaggcc gtacgcgctg tgctggacga gattgaggtg
     1681 ggtgaaaaac cgttcgtgct tgcactgaac aagatggatc tcgcaacgcc catgcaggcg
     1741 tcagagcttg aaacgcgatt cggccgcggc gtgcgtgtct ccgcccgcac cggtgagggc
     1801 atcgaagaac tgcttcacgc gatcgacgcc aacctgatgc cgcagcgctc gcgggtgcat
     1861 ctgcgcattc cccaggatca gggcgggatt gtggcccggg tgcacgattt cgggcgggtt
     1921 atccaacaaa attatgaaga caacgtggta gtgctggagg ccgaagtcga tgtagcgttg
     1981 cggcaacagc tcgccgacta cattgtatga ccgccgcccg gattgtgccc gtaacggaat
     2041 tgggcgggcg agcccaggtt cccgatcttc gggaaccgct tctccgcctg cagtaagcgc
     2101 tggcaaagcc ggaatcgccc ggtgtagacc gcgggctgtg tggtttttgg gcacatagtt
     2161 ctcagggaat cagcacgagg agctcgagaa tgatgaggcc agaggacgac cattcgcatg
     2221 tggagccggc cagcgcgact gccagagatc agcgcgcccc gctgacgttt tggatctttc
     2281 ccgcagcact cgctctggga gtgcttggcg acctcctgct tcgtgcctgg ccctacgggc
     2341 tgaatgccgc cctgtgggcc gtggcaacgc tggcgtgtgc ggcggggctc ctgcattggc
     2401 gtgaacggcg attgacgccc tggaccacag tcttcctggc cgcgagcgca gcgttcgcgc
     2461 tttgtctagc ctggcgggac agcgcttatc tcaaatcgct tgcgctgtca gtatgctggt
     2521 gagcgaaggg atgggtaccg gggtttcttg acaaaccgct tgtagctgtc gacgatttct
     2581 gttttgactt ttccgcaggc ttgccggagg gcagcaacct ctttggcgtc ggtcaatgcg
     2641 ccgaattcga ggtgcacttc ctggctttgt cccgcgcgaa gggagtgaag aaggccttgc
     2701 ttttcatcaa cggcgcggcc ctcgactccg cagttgcacg gttcgaaacc gaccacgtag
     2761 tcctgttcgc ccatctgttt ccactcgaca aatcggggca gttctttttt gttgtatttg
     2821 aggtacacgc caaagccttc gcccttgccc ttgaacccgc tgttgacgat cgccgcggtg
     2881 acattgcttc gagcatcgcc caccatgtcg tgataataga ccttttctct gtagccccgg
     2941 gtgggagggt cgagtttata ccagttctgc ttcccatttt cggcttctgc gtcccgcggg
     3001 gcggcatagc ggctgggagc gatgatctcg gcgccctggt ccacggccgg ccaaccgatg
     3061 ttgcagtggt aaagcagcat gtatttggcc ggtttgaacc catcgttggt cacgctgtca
     3121 tggatccaga agcgtttttc acccagccgg gtagacacgg tgcgcgtcaa ggtcagattt
     3181 tctccaaaca cgaccgtctc acgcatagtc cctgagacac tcaggacgta gtcgttgccc
     3241 tgccattcct gggtgatttt gatgttcttc gcgggggtgt tgccgatgcg gccgtgcagg
     3301 ccggtaccca ggagggcgct ggcttcgtcc ggcgcaccca cattcatcag gccgcaggtc
     3361 gtgacgagcc cgccgaaata gctgcggagc caccggagcc cttcggcctc gaaatattgc
     3421 ggggcgacgt cgccggtggt ggagcgccag cccatggcct tgccgttgaa cgacgcggcg
     3481 gagatgtcga ggcaccggtc caggagcacg gtgagctcga gtcccgctcc ggtatgaatg
     3541 atcgccgcgc gggtcggccg ttcattgccg tcatccagct gcacggtccg gatgcccgca
     3601 atctggtcca tgttgcccac gcgcgtgcgc agttcttttg tgctgtattt ctttccgtag
     3661 atttccatgg tgactccctc cgcatggaat ggctgttgta ttgaccgata ctctaagcct
     3721 ttgaggcgcg gcatgcaaaa acgcgctgga aaggcgtgga ggggatccgt acactgagcc
     3781 gtgccatgga tacgacagcc actctgatcg
//
LOCUS       DAOPBQ010000374         3806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_19518_length_3806_cov_4.003732, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000374 DAOPBQ010000000
VERSION     DAOPBQ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19518_length_3806_cov_4.003732"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..3470
                     /locus_tag="PKW60_11415"
     CDS             108..3470
                     /locus_tag="PKW60_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HPK00086.1"
                     /translation="MTRFSGFALCLLAICLNVEAAPGTAGIRLIDAKYPVEDRIIASA
                     VVEAPASAGEDATEAIQSAIDAVAALGGGTVFLPEGRYLLKGHLLLRNAVTLRGEWLP
                     PDREGVVGTVLMPVEHHGNAEGESAIVMQCGSGLRELSIWYPEQKPDAIVPYPWAIET
                     SPVAGVDNYTLHNVTLVNVYRGFKTGPETNELHTLKNVYMTALETGVFIDSCTDIGRV
                     IDIDISPRWWLESKLPGSPAGESAAASLRAQVAREATAVDMGRSDWEYLYGLHIEGYQ
                     RGLVIRQGAQGTTNAVLYGSVFENCATAVHLEKLNGIGLAAVGCRFEGSQHAVHATAA
                     FDTVSQFNTCTFTGLDGHGVLLEGDGMLTFQNCVFDAWTTSGVEAAAGAVSLLGCEFQ
                     GAASPVKLGERVNRARLLGNKFAGQPVIENAATNADVMITHHAMTFEVPDVSRPPAPP
                     APAPGSRDVVLVTGYGARPDLDDNTEAFQGALTAAERLGGATVYVPAGYYRFRGALTV
                     PAGVELRGCFDVPHHTQSGGSVLMPMGGQGDEGGAPFIALSAKSGLRGLTIWYPEQNL
                     LDVQPYPWAVRSLGPACWLIDVTISNAWQGVDFWTHPSEGHYISYLGGAFLRRGLFVS
                     KSAGDGWVVDVQMNPHYGLRLPHDLPRPAYSKDTFEPIIDQQRGNLEAMVFGRCAREH
                     IYRTFLYAAYDGIAFRDDGGGSNARILMHGTDTGSRCAVLSACGGEGLDFLLMQLVPL
                     GKYEVGAIIAEDQFAGRARFFTSQMWAGHCSGQFSGVGSVLIQQLNSISGGMTCEAGT
                     FRLENAVFHHALEPHVAIGRGCMKADLIANAALSGEFSVENAAGERARCLANTVSRHP
                     SSFEPGALATGWETGEPEGLANTPAAEGGGLRGVEEGFCAPAEGTSHHGARALRVAGE
                     ARDGEHSYIYFSLFEEPLRVYSDTQLRYWFLPANEAGRHVGVDMLFDDGSTLRDSRIK
                     TAEGENIHPGTPKGAVGEWQPVTVPLGRHAGKLIRRIMAAYDQHGATGPFEAFIDDLE
                     ITTAVIPELAGLAVSPRGGLCSAETRVSFAVPSGFRVRYSLDGIKPGSDAPHYVEPIA
                     FPGPGLWDLRYIVEKPDGTPLPWVFGDLYDAR"
     gene            complement(3571..>3806)
                     /locus_tag="PKW60_11420"
     CDS             complement(3571..>3806)
                     /locus_tag="PKW60_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00087.1"
                     /translation="ELRVKKLDDAEEHLGRALQMRPGDPTLLLDFGALLLEKGNTSAG
                     QRYIRMAIDYADRLGLAFPRKDEAQRLIETESP"
BASE COUNT          703 a         1106 c         1280 g          717 t
ORIGIN      
        1 ggagcgtgcc acaggtcgtg agagtcgatc cgcgcgcggc ttcatggtac aacggttggc
       61 gggcagaagg cctcgcttaa tcaattgcgg aagggagatc ccgaatcatg acgcggtttt
      121 cagggtttgc actgtgttta ctggcgattt gcctgaatgt ggaggcagca ccggggacgg
      181 cgggaatccg cctgatcgac gcgaaatatc ccgtagagga ccggatcatc gcctcggccg
      241 tggtcgaagc gcccgcatca gccggggagg atgcgacgga agcgattcaa tcggcgatcg
      301 atgcggtggc ggctctgggg ggcggcacgg tgtttttgcc ggaaggccgc tatctcctca
      361 agggccatct gctgctgaga aatgctgtga ccctgcgcgg agaatggctg ccgccggaca
      421 gggaaggcgt ggtggggacg gtcctgatgc cggtcgagca ccatgggaac gccgaaggcg
      481 aatcggcgat cgtgatgcaa tgcggctcgg gactgcggga attgtcgatc tggtatcccg
      541 agcagaagcc ggacgccatc gtaccctatc cctgggccat cgagacgtcg ccggtagcgg
      601 gtgtggacaa ctacaccctc cacaatgtga ccttggtcaa tgtctaccga ggcttcaaga
      661 cgggcccgga aaccaatgag ctgcacaccc tgaaaaatgt atacatgacc gcgctcgaga
      721 ccggcgtatt catcgattcc tgtacggata tcggccgagt tattgatatc gacatcagtc
      781 cgcggtggtg gctcgaatcg aagctgcccg ggagcccggc cggcgaatcc gcagcggcca
      841 gcctgcgcgc gcaggtggcc cgggaagcca cggctgtcga catgggacgc agcgactggg
      901 agtatttgta tggactccac attgagggat accagcgggg cctggtcatc cgccagggtg
      961 cacagggcac gaccaacgcc gtgttgtacg gaagcgtttt tgagaattgc gcgacggcag
     1021 tccatctcga aaaattgaac ggcatcggcc tggctgccgt gggatgccgg tttgaaggat
     1081 cgcagcatgc cgtccatgcg accgctgcgt ttgatactgt gtcgcaattc aacacgtgca
     1141 cgttcaccgg cctggatgga cacggcgttc tgctcgaagg cgacggcatg ctgacgttcc
     1201 agaattgcgt gtttgacgcc tggacgacgt cgggcgtgga agcggcggcc ggcgcggtgt
     1261 cgctgctggg atgcgagttt cagggcgctg ccagccccgt gaaactgggc gagcgggtca
     1321 accgcgcccg ccttctcggc aacaagtttg ccggccagcc ggtcatcgaa aacgccgcga
     1381 cgaacgccga tgtgatgata acccaccacg cgatgacgtt tgaggtgccg gatgtctcgc
     1441 ggccacccgc gccgccggct cccgcaccgg gaagccgcga cgtagtgctg gtgaccggct
     1501 acggggcgcg gccggacctc gacgacaaca cggaggcgtt ccaaggggca ttgacggctg
     1561 cggagcgtct gggcggggcg accgtctatg tccccgcggg atattaccgg tttcgcgggg
     1621 cactgacggt tccggcgggt gtggagctgc gcggctgttt cgacgtgccg caccacacgc
     1681 agtccggcgg gtcggtattg atgccgatgg gcgggcaggg cgatgaaggc ggtgcgccgt
     1741 ttattgcatt gtccgcaaaa tcgggtctgc ggggcctgac gatctggtac ccggagcaga
     1801 atctgctcga cgtgcagccg tatccgtggg cggtgcgttc gctgggaccg gcatgctggc
     1861 tcatcgacgt gacgatttcg aatgcctggc agggggtgga tttctggacg catcccagtg
     1921 aaggccatta catcagctac cttggcggcg ctttcctgag gcgtgggctg tttgtgagca
     1981 aatccgcggg cgacggctgg gtggtggatg tgcagatgaa tccccattat gggttgaggc
     2041 tgccgcacga tctgccccgg ccggcgtata gcaaagacac cttcgagccg atcatcgatc
     2101 agcagcgcgg caatctcgag gcgatggtct tcggccgttg tgcgcgggaa catatctacc
     2161 gcacattcct gtatgccgcg tacgacggta tcgccttccg cgacgacggg ggcggaagta
     2221 atgcgcgtat tctcatgcac ggaaccgaca ccggaagccg ctgcgccgtg ctaagcgcgt
     2281 gcggcggcga agggctcgat tttctactca tgcaactggt ccctctgggc aagtacgagg
     2341 tgggcgcgat catcgccgaa gaccaatttg ccggacgggc ccggtttttt acttcgcaga
     2401 tgtgggcggg ccactgcagc ggccagttca gcggagtggg ctccgtcctg atacagcaac
     2461 ttaacagtat cagcggcggg atgacctgcg aggcgggcac ttttcgcctt gaaaacgccg
     2521 tgtttcacca tgcattggag cctcatgtcg ccattgggcg cggctgcatg aaagcagacc
     2581 tgatcgccaa tgccgccctg agcggagagt tttcggtcga aaacgccgcg ggagagcgcg
     2641 cccgttgcct ggccaacacc gtctcacggc acccgtcgtc gttcgaaccg ggcgccctgg
     2701 ccacgggctg ggaaacaggc gaaccggaag gcctcgcgaa caccccggcc gctgaaggcg
     2761 gcgggttgcg gggcgtcgag gaaggcttct gtgcgccggc ggaggggacg tcgcaccacg
     2821 gcgccagggc gctccgggtg gcaggcgagg cccgcgatgg agagcacagt tacatctatt
     2881 tttccctgtt tgaagagccg ctgcgagtct attcggatac gcagctccgt tactggttcc
     2941 ttcccgcaaa cgaggcgggg cggcacgtag gggtggatat gttgtttgac gacggctcga
     3001 cgttgcgtga ttcccgaatt aaaaccgcag aaggcgaaaa cattcatccc ggtacgccca
     3061 aaggtgcggt gggcgaatgg cagccggtta ccgtaccgct gggccggcac gcggggaaat
     3121 tgatccgccg gatcatggcc gcctatgacc agcacggcgc cacagggccg tttgaagcct
     3181 ttatcgacga tctcgaaatc acaaccgcgg tgattccaga gctggcgggc cttgctgtga
     3241 gtccccgggg tggcctttgt tccgccgaaa cacgcgtgtc tttcgccgta ccgtccgggt
     3301 ttcgcgtgcg ctattcgctc gacggaatca aacccggctc ggatgcacca cattatgtgg
     3361 agccgatcgc gtttcctgga ccagggctgt gggatttgcg atatatcgtg gaaaaaccgg
     3421 acggcacacc gctgccctgg gtctttggag acttgtacga cgcccgctga ctcggaatgg
     3481 cgctccgcag tgcatcgaca tgacaaaacc gggccgcccg cttcaaggcc ggcccggttc
     3541 tgagtcagat ccccaagaaa cggaaaccgg tcacggcgat tccgtctcga tcagccgctg
     3601 cgcctcatct ttccggggaa aagccagtcc cagccgatca gcatagtcaa tggccatgcg
     3661 gatgtagcgc tgcccggcgc tggtgttgcc tttttcaaga agaagggcgc cgaaatccag
     3721 aagcagggtt gggtcgccgg ggcgcatctg cagcgcgcgc ccgagatgct cttccgcatc
     3781 atccagtttc tttacccgca gctccg
//
LOCUS       DAOPBQ010000375         3789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_19614_length_3789_cov_3.645956, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000375 DAOPBQ010000000
VERSION     DAOPBQ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19614_length_3789_cov_3.645956"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKW60_11425"
     CDS             complement(<1..369)
                     /locus_tag="PKW60_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00088.1"
                     /translation="MNIGRDTSWYYSLFWQSTSSGRRLRRAVCASIFLHIVILAAFFF
                     VLIYHKPPKPIEAYQVQLIPADQDVEAEPTEEAIEEPPPPPPPPPPPPPEPEPEPEPE
                     PKPEPKPEPKPEPKPEPKPEP"
     gene            555..1982
                     /locus_tag="PKW60_11430"
     CDS             555..1982
                     /locus_tag="PKW60_11430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK00089.1"
                     /translation="MRLGFLRNDPPIRAARGLLGIYTRWLLWYLLAVAVVWVLGFEGI
                     YYHPTPFYGILRPDFDLTTWLWQSLAIGVFLAGFFLWRLCLRQADWFEGAWSAKRMRI
                     FVAGLVVFAVLFPMALAMVREGPSGIPGPYLRHDKEYIGDIGKGLTIRGLFHDYLKMH
                     PHLSMHSKVHPPGPVALLWAFSLVLFSQGAWPMSIATILFGATAVIPLYLWVRDLAGP
                     RVALTACCLYVVTPSIVLFSATSADILFMPFTVTALFLFWRALHQRSIGYAVAAGFTY
                     ALMSLMSFSLLTIGAFFGFVGLWRLGEPKLRIAVVQTAALMLGAFVATHLAVRWWSGF
                     DVFACFQACKAQFDTDQANLDLLTPRFPGWSWKLLNPVCWLYFAGIPVSVLFLRRLVK
                     PEAGTRTLFLIFALTLVVLNLLYLARGEGERSAMYIIPFLVVPAAHLIDRIGHETRSI
                     IPLAATLCFLAAQCWFTEMMLYTYW"
     gene            1990..2730
                     /locus_tag="PKW60_11435"
     CDS             1990..2730
                     /locus_tag="PKW60_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK00090.1"
                     /translation="MWNGKRVSVIFPTYNEKDSIREAIEDFFASGCVDEIVVVNNNAA
                     EGTDDEVAQTKARLVYEVRQGYGNAIQKGLAEAAGDLLIIAEPDGTFSGHDVVKLLAY
                     SDDVPVVFGTRTQRELVWEGANMGFFLKWGNWAVGKLMEFLFNTTLLTDVGCSMRLFH
                     REAYEAISPQFAIGGSAFGPHLMLLTILNGLPFIESPVNYRKRVGESSVTGSKIRAFI
                     LGLEMILMILRYRVKSWFGWRPPRVREP"
     gene            2808..3635
                     /locus_tag="PKW60_11440"
     CDS             2808..3635
                     /locus_tag="PKW60_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HPK00091.1"
                     /translation="MPQNPVPFLKWAGGKRQLMPELLRRIDRLGAFGGYYEPFVGGGA
                     LFFELWNRGRLAGPVRLSDNNPNLIVAYEGVKHEVERVIELLREHARRHSKEHYYATR
                     AARPSRRAEQAARLIYLNRTCFNGLFRENSRGEFNVPMGRYANPRICDEANLRAVAEA
                     LRMVQVEVAPFQSVVESAAPGDLVYFDPPYHPVSATSNFTAYQKGGFGEEDQRALAAV
                     FRALDAQGVHVLLSNSWTDFIRELYADFTLEQVSASRSVNSRADRRGAVWEVLVRNF"
BASE COUNT          667 a         1043 c         1215 g          864 t
ORIGIN      
        1 gggttctggt tttggttcgg gcttgggctc cggcttgggc tccggcttgg gctctggttt
       61 gggttccggt tctggttctg gctcaggttc cggcggcggc ggaggcggcg gtggcggtgg
      121 cggcggaggt tcctcgatgg cctcttcggt gggctcggct tcgacatcct ggtcggccgg
      181 gatgagctgt acctgatacg cctcgatggg ttttggcggt ttgtggtaga tcaggacaaa
      241 aaagaatgcc gcaagaatga caatgtggag aaaaatcgac gcacagaccg cccggcgcag
      301 ccgtcttccg gaggaagtgc tctgccagaa cagactgtag taccaggaag tatcacgccc
      361 gatgttcatg tttcagtcgt agtcaatcca gcgaaaatag taccgcacat atactggtaa
      421 gtcaaagatg ttaccacaat cccttgtggt ttgtcgcgcg gcgcatggat aattaatccc
      481 cggccgccgt gcctgggttc cgccattccg ggctggagtg gattgcacgg accgtctcta
      541 ttacaatacg gcgcatgcga ctggggttcc ttcgaaatga tccaccgata cgggccgcgc
      601 gaggcctttt gggcatctac acgcgctggc tgctgtggta tcttcttgcc gtggccgtgg
      661 tatgggtcct gggcttcgaa ggtatttact atcacccgac gccattttac ggcatattgc
      721 ggcccgattt cgacttgaca acctggctgt ggcagagcct tgctatcggt gtgtttctgg
      781 ccggtttttt cctgtggcgc ctgtgcctgc gtcaggcgga ctggtttgag ggggcgtggt
      841 ctgcgaagag aatgcgcatc ttcgtggcgg ggctggtcgt gttcgccgtg ttgtttccga
      901 tggcgctggc aatggtccgc gaaggtccct ccggaatccc gggaccttac ctgcggcacg
      961 acaaggaata cattggcgat atcgggaaag ggctcacgat acgggggcta tttcacgatt
     1021 atctcaagat gcatccgcat ctctcgatgc actccaaagt gcatccaccg ggtccggtcg
     1081 cgctgttgtg ggcgttttcg ctggtgctgt tcagccaggg cgcttggccc atgtcgatcg
     1141 cgacgattct tttcggagcg acggccgtga ttccgctcta tctctgggtc cgggacctgg
     1201 ccggtccgcg cgtagcgctg acggcgtgtt gcctctatgt cgtgacgcca agcatcgtgt
     1261 tattttccgc gacatcggcg gacattctgt tcatgccgtt cacggtgacg gcgctgtttt
     1321 tgttctggcg cgcgcttcac cagagatcga tcggatatgc ggtggccgcg ggattcacgt
     1381 atgcgctgat gtcgctgatg tcgttttctc ttctgacaat cggggcgttc ttcggattcg
     1441 tgggcttgtg gcggctcggc gagccgaagc ttcgcatcgc ggtagttcag accgcggcat
     1501 tgatgctggg ggcctttgtg gcgacccacc tggctgtacg atggtggtcg gggtttgacg
     1561 tattcgcctg ttttcaggcc tgtaaagcgc aattcgacac ggatcaggcc aacctcgatc
     1621 tgctgacgcc gcgttttccc gggtggtcct ggaagctgct caacccggtc tgctggctct
     1681 attttgccgg gatcccggtg tcggtactgt ttctgcggcg cctggtgaag cctgaagcgg
     1741 gtacgcgcac gctgtttctg attttcgcgc tgacgctggt ggtactgaac ctgctttatc
     1801 tggcccgcgg cgagggcgaa cggtcggcaa tgtacatcat cccgtttctg gtggtgccgg
     1861 cggcacacct gatcgatcgc attggacacg aaacccgcag catcattccg ctggccgcca
     1921 ccctgtgctt cctggctgcc cagtgctggt ttaccgagat gatgctgtac acctattggt
     1981 agacagacaa tgtggaacgg caagcgagtc tcggtcatct tccccaccta caatgaaaaa
     2041 gactccatac gtgaggccat cgaggacttc ttcgcgtcgg gctgcgtcga tgagattgtg
     2101 gtggtcaaca acaacgcggc cgagggcacc gacgacgaag tggcgcagac gaaggcgcgt
     2161 ctggtgtacg aagttcggca aggctatggc aacgccattc agaaaggtct ggccgaggcc
     2221 gcaggcgacc tgctgattat cgccgaaccg gacgggacat tttcggggca tgacgtggtc
     2281 aagctgctcg cgtacagcga cgacgtgccg gtggtgttcg gcacgcgcac gcagcgggag
     2341 ctggtgtggg aaggcgcgaa catggggttc ttcctgaaat gggggaactg ggcggtcggt
     2401 aagttgatgg aatttctttt caatacgacg ttgctgacgg atgttggctg ctcgatgcgc
     2461 ctgtttcacc gggaagcgta cgaggcaatt tcgccccagt ttgccatagg aggatccgcg
     2521 ttcgggccgc atctgatgct gttgacgatc ctcaacggtc taccatttat cgagagtccc
     2581 gtgaattacc gcaaacgcgt gggcgaatcg tcagtgaccg gcagcaaaat ccgggccttt
     2641 attctcgggc tcgaaatgat tctgatgatt ctgcgttacc gcgtgaaatc ctggtttgga
     2701 tggcggccgc cgcgagtccg cgaaccgtga aacgcacggc atccacacca gagcagacgg
     2761 cgctgtggac ttcagaaatg gtggagacct cacaagcaga cggcgccatg ccgcaaaacc
     2821 ccgtgccatt cctcaaatgg gccggaggca aacgccagct gatgccggaa ttgttgcggc
     2881 gcatcgaccg tctcggcgcg ttcggcgggt attacgaacc gtttgtgggg ggcggcgcat
     2941 tgttttttga gttgtggaac cgcggccggc tggccggccc ggttcgcctg tcagacaaca
     3001 accccaactt gatcgtggcg tatgagggcg tgaagcacga agtcgagcga gtgatcgaac
     3061 tgttgcggga acacgccaga cgtcacagca aagagcatta ctacgccacg cgcgcggccc
     3121 ggcccagccg gcgggcggag caggccgctc gactgattta cctcaacagg acgtgtttca
     3181 acgggctttt tcgcgagaac agccggggcg aatttaacgt gccgatgggc cgctacgcga
     3241 atccccggat ttgtgacgag gccaatctgc gggccgtggc cgaagcgctc cgcatggttc
     3301 aagtcgaggt agcgcccttc cagtctgttg ttgaatccgc cgcgcccgga gacctggtct
     3361 attttgatcc gccgtatcac cccgtctcgg ccacgtccaa tttcacggcg taccagaaag
     3421 gcgggtttgg ggaagaagat cagcgcgcac tggccgcggt gtttcgtgcg ctggacgccc
     3481 aaggcgttca tgtgctgctg tcgaattcct ggacggactt catccgtgaa ctctacgcgg
     3541 atttcacgct cgaacaggtc tcggcgtcac ggagcgtcaa cagccgtgca gacaggcggg
     3601 gtgccgtctg ggaagtgctt gtgcgcaatt tctgaaggct gcggtgcgtt ccgcggccga
     3661 agcggcacgg gtcaccctgt gacctcgatc tcgccggcct gaatcatttc aatgaacagc
     3721 ttgacgacgc gggcgtcgaa ctgagcaccg gaacagcgtt tcaactcctc gatgatccgg
     3781 tcgcagggc
//
LOCUS       DAOPBQ010000376         3773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_19714_length_3773_cov_3.687735, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000376 DAOPBQ010000000
VERSION     DAOPBQ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19714_length_3773_cov_3.687735"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /gene="hemG"
                     /locus_tag="PKW60_11445"
     CDS             complement(<1..1040)
                     /gene="hemG"
                     /locus_tag="PKW60_11445"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HPK00092.1"
                     /translation="MKQAIVVGGGVTGLCAAFYLARDLGREHVTLLEAGDSAGGTTRS
                     EAADGYACDCGPNGFLDREPATLEWIEALGLSGELLRANEFAKRRFIYTGGRLHEVVG
                     PPRFFFSPMLSIKGRLRLMCEPFIPGRRDHTGETIWAFAARRIGTEAANILVDPMASG
                     VFGGDARVLSLEHCFPRMAAVERDYGGLTRALISKRLQRMAVSPLGPSGRLTSFREGI
                     GRLPVEAAKALGDVLRTGERVLRITTTSEGHYALESSSGETYYARSVIFAAPSYAAAE
                     CCREFDVSAGKALESIRYADIAVVCTGYPRAAVRGNTNGFGFLVPRHENLRVLGCLWT
                     STMFPEQAPPERT"
     gene            complement(1049..2461)
                     /gene="hemN"
                     /locus_tag="PKW60_11450"
     CDS             complement(1049..2461)
                     /gene="hemN"
                     /locus_tag="PKW60_11450"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HPK00093.1"
                     /translation="MNETADNTHRPESVEVTRDLLLRHDRPGPRYTSYPTVPAWSAQF
                     GPPEYRACLELAASRSGEPLSLYVHIPFCRKRCAFCGCNSIATQEPGVAGTYLDHVEL
                     ELQMLSELLAWSRPLKQIHWGGGTPTYLDMAQLERLFQAIADRFDIPEDAEISIEADP
                     RVTTVDQVTLLRKLGFNRISLGVQDLDERVQAEIGRHQTEAQTRALVTLCREAGFEGI
                     NLDLIYGLPGQTPGQWATTISRIIEMQPDRLAVYSCAYLPDKMLNQRRLDSAKMPTGP
                     EKYALFATARQMFTAAGYRAIGMDHFALPHDELTRAMDERRLHRNFMGYTAVPSSDMV
                     ACGISAIGEVGGGYAQNEKELAPYYEALDAGRFATALGCRLTPDDEIRRWTIRELMCN
                     FHVSFTQLQTRYGVEFEDYFIGELKDLREFEREGFVTWESTGLTVLPLGQIFVRNIAM
                     AFDTSLKGKGAPPERFSRTV"
     gene            complement(2526..2834)
                     /locus_tag="PKW60_11455"
     CDS             complement(2526..2834)
                     /locus_tag="PKW60_11455"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HPK00094.1"
                     /translation="MAGWVRVCALEELPPGTQREVVCGMGEPVALFNVGGKIHAISGQ
                     CPHQGGPLAEGRVDNGSVSCPWHGWRFSLTPSTTPPNDLIRHYRTEIRDDGLYIAPPR
                     "
     gene            3031..3645
                     /locus_tag="PKW60_11460"
     CDS             3031..3645
                     /locus_tag="PKW60_11460"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPK00095.1"
                     /translation="MAFQLPELPYAPDALEPYIDARTMEIHHSKHHGGYVAKLNAALE
                     GVSGFEGKTVEDILRKITAVPEKVRQAVINHGGGHANHSLFWQLMGPGKGGDPSGAVA
                     DAINAEFGGVGPFKEKFADAAANRFGSGWAWLVFNSSGKLQVLSTANQDSPLMDGHTP
                     ILGLDVWEHAYYLKYQNRRPDYIDAWWHVVDWAKVNELYRAAKG"
BASE COUNT          760 a         1174 c         1165 g          674 t
ORIGIN      
        1 gtgcgttccg gaggggcctg ctccggaaac atggtcgatg tccagaggca tcccagcacg
       61 cgaagattct cgtggcgcgg caccagaaaa ccgaagccgt tggtgtttcc gcgtacagcc
      121 gcacggggat accccgtaca cacaaccgca atgtcggcgt aacgaataga ctcgagggct
      181 ttgcccgcgg acacatcaaa ctcgcgacaa cattccgcgg ccgcgtacga aggggccgca
      241 aatattacag agcgcgcgta ataggtctcg ccgctgctgg attccagggc atagtggcct
      301 tcggacgtcg tagtgatgcg gaggacgcgt tcgccggttc gcaggacgtc acccagcgcc
      361 ttcgccgcct ctacgggcag ccgcccgata ccttcccgaa agctggtcag tctgcccgaa
      421 ggccccaggg ggctgacggc catcctctgc agccgtttgc tgatcagcgc ccgggtgagg
      481 ccgccgtagt cgcgttcgac cgccgccatc cgcggaaagc agtgttcgag cgacagcacc
      541 cgcgcatcgc cgccaaatac cccgctggcc attggatcga cgaggatgtt ggcggcctcc
      601 gtgccgatgc ggcgcgccgc aaacgcccag atcgtctcgc ccgtatgatc gcgccgccca
      661 gggataaacg gctcgcacat cagcctcagg cggcctttta tggaaagcat cgggctgaaa
      721 aaaaagcggg gcgggccaac cacttcgtga agccgcccac cggtgtaaat aaatcggcgt
      781 ttggcgaatt cgttggcccg gagcaactcg ccggacagcc caagcgcctc tatccattcg
      841 agggtggcgg gttcgcggtc aaggaagcca ttggggccgc aatcgcaggc atacccatcg
      901 gccgcttcac tccgggtcgt cccgccggcg ctatcaccgg cttcgagcag agtcacatgt
      961 tcccggccca ggtcccgggc gaggtagaac gcggcgcata atccggtcac cccgccgcca
     1021 acgacaatgg cctgcttcac tggatgcatc ataccgtacg cgagaagcgc tccggcggcg
     1081 ctcctttgcc tttcaggctt gtgtcaaagg ccatagcgat gttgcggaca aatatctggc
     1141 caagcggcag gaccgtgagc ccggtgcttt cccaggttac aaacccttcg cgctcgaact
     1201 cgcgcaaatc cttcaactcg ccgatgaaat aatcttcaaa ttccacgcca taccgcgtct
     1261 gcaattgcgt gaaactcaca tggaagttgc acatcagctc acgaatggtc cagcggcgga
     1321 tttcatcgtc gggtgtaagc cggcagccca gcgcggttgc aaaacggccc gcgtcgagcg
     1381 cttcgtagta cggtgcgagc tctttttcgt tctgcgcata accgccgccc acttcgccga
     1441 tggccgagat gccgcaggcg accatatctg acgagggcac ggccgtgtag cccatgaaat
     1501 tgcgatgaag acgccgttca tccatggcgc gggtcaattc atcgtgcggc agcgcgaaat
     1561 ggtccatgcc aatggcccgg tagcccgccg cggtgaacat ctggcgtgcc gttgcgaaca
     1621 gggcgtattt ttcgggaccc gtgggcattt tcgcggagtc gagccggcgc tggttgagca
     1681 tcttgtccgg gagataggcg cagctgtaga ccgcgaggcg gtcgggttgc atctcaatga
     1741 tccgggagat cgtagttgcc cactggccgg gggtctgccc gggaaggccg tagatcaaat
     1801 ccaggttgat gccttcaaac cccgcctcgc gacacagggt caccagggcg cgggtctgcg
     1861 cttccgtctg gtggcggccg atctcagcct gaacccgctc atccaggtcc tgcaccccca
     1921 ggctgatccg gttgaatccg agctttcgga gcagagtcac ctggtcaacg gtggtgacgc
     1981 ggggatccgc ctcgatcgag atctccgcat cctccgggat atcaaaccgg tctgcgatgg
     2041 cctggaagag gcgctcgagc tgcgccatgt ccagataggt gggggtgccg ccgccccaat
     2101 ggatctgctt gagcggccgg ctccaggcca gcagctccga cagcatctgg agttccagct
     2161 cgacatggtc cagatacgtg ccggccacgc cgggctcctg cgtcgcgatc gaattgcacc
     2221 cgcaaaatgc gcagcgcttc cggcaaaaag gaatatgcac ataaagcgac aacggctcgc
     2281 cggatctcga cgctgcgagt tccaggcagg cacgatactc cggaggaccg aactgcgccg
     2341 accaggcggg cacggtggga tagctggtgt agcgcggtcc aggccgatca tgccggagca
     2401 gaaggtcgcg ggtgacttcc acggattccg gccggtgtgt gttatcagca gtctcgttca
     2461 tgagcactct gcagcatcag taaaaagatt atagcgggaa acggccggga ttgtgaaacc
     2521 tgccgtcacc ggggcggggc aatatagagc ccgtcgtccc gaatctcagt ccggtagtgc
     2581 cgtatcaagt cattgggcgg cgtggtgctg ggcgtgagcg aaaagcgcca accatgccag
     2641 ggacacgaca cgctgccgtt gtccacgcgc ccttccgcca gcgggcctcc ctggtgaggg
     2701 cactggccgc ttatagcatg gatttttccg ccgacgttga aaagcgcgac cggctctccc
     2761 atgccgcaca cgacctcgcg ctgagtcccc ggcgggagct cttcaagcgc gcaaacccgc
     2821 acccatcctg ccatcgaagg atatcctcca atccccttgg gatttgttcg cgcgcgggca
     2881 gtctagcgcg tggtcaggcc cgcgcgcaaa tcgtctgaga gaacaagaag tctgccacgg
     2941 aaacaatcgc gggcgcacgg gtgttattcc cttgatgcgc ccgaaaggcg gcgcgcacag
     3001 cataacagag ccgaaatgga ggaactgacc atggcatttc aattgcctga actcccctat
     3061 gcgcccgatg cgctcgaacc gtatatcgat gcacgcacga tggagatcca tcacagcaaa
     3121 caccacggag gatacgtcgc taaattgaac gcagccctcg aaggtgtgtc aggttttgaa
     3181 ggaaagaccg tcgaagatat cctgcgcaag atcactgcag ttcccgagaa agtccggcag
     3241 gcggtgatca atcatggcgg cggccacgcc aatcacagcc tgttctggca gctgatgggt
     3301 cccggcaaag gcggcgaccc ctccggcgca gtagcggatg ccataaatgc cgaatttggc
     3361 ggcgtgggcc cgttcaagga aaagttcgcg gacgccgcgg cgaaccgctt cggcagcggc
     3421 tgggcctggc tggtgttcaa cagcagcggc aagctccagg tactcagcac cgccaaccag
     3481 gactcaccgc tcatggacgg acacacgccc atcctcgggc tcgacgtctg ggaacacgcc
     3541 tattacctca agtatcagaa caggcgcccc gattacatcg atgcctggtg gcacgttgtt
     3601 gattgggcga aagtgaacga gctctaccgc gccgcaaagg gctgatcgaa ctgctcgcgg
     3661 tgcgcgcagg tcctcaggga agccgcttcg ccgaggtcga gtagatggat ttgagctggc
     3721 ggctccggcc gggttggagc ggatcctgtt tcgggattag ctcgagattg atg
//
LOCUS       DAOPBQ010000377         3762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_19788_length_3762_cov_4.787429, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000377 DAOPBQ010000000
VERSION     DAOPBQ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19788_length_3762_cov_4.787429"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..938
                     /locus_tag="PKW60_11465"
     CDS             126..938
                     /locus_tag="PKW60_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK00096.1"
                     /translation="MGFLLVLKAEIVRGLIIMRRYWFATITGLIISYGFLMLVILAFV
                     SNQAALAEWSESIFDKVVGFLVGMLAMGLVGMFSQGLQGMARTGELEQVCMSPHGLVT
                     NFLARSLVAAVMSILTYTILLSLLAASLKGKIYLEPVSTALVLALTYTNLIGFGFMVG
                     GLVLVFKQTGQIAMIIRLVLLIIAIGSEEVAKLPAWFRAIAHCVPITDAAISLKYVLI
                     KGQHIDGRYSSILVQPSFYYLILNAAAWTLIGIACFRYLENWSRDKGTLGAY"
     gene            938..2878
                     /locus_tag="PKW60_11470"
     CDS             938..2878
                     /locus_tag="PKW60_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HPK00097.1"
                     /translation="MHGAALHRFELNGKRYAIDPETCFCFECDEICWDVLALYPHETI
                     SRIVHELKQKHSEREVYEVVGELEWLRNVKSILPRPTPEELQKRYEIERGLKRLTVEL
                     PEETSVAAPAPEKTGWFRKRREVSSAGLSSLGRDAAHLLLNRSGNQKVLELEFLEHGC
                     IHDLDAVAALCADVLRAARLAGKELTAAVRIQDLRLEPEPGALKGHTVSARLELTEPD
                     AAQSALAPLAAGNVFTLAQLAKALQPGASGVQGRIIVRPDTPDFGGVVQTLDEAGFKV
                     IELDLDATFVAHPDLPPAEVLPGLSASAIYYARRLLEGHYFRLDPIASLFYRIYEGQP
                     IQRADPIGTNELAVDSRGDIYASWRVFGNERFRHGSLQNATIDEAAIKRYDDMGVPTT
                     GPCLRCWVRHLCGGGRAAVHDALSGNFHQPHEPWCDAQRSWMAAAVSAFNVLAAEGIN
                     FTRIYQTLTPSAGKRPTLSMLTMLRAAIGLSIRMRPVEESDAELLVRWENWNPAAYFL
                     FNEKGAFVATRYEREMDALHPQTFDQELVLTRKDGTPFGLLKLRAEKVAETASAWLYF
                     RNEADYAAADLRKGLAFLLKQAGQQQGLRRITVSTAEYEHSLQHFLEQCGFTREGTLR
                     EALYLHGTYRDVHVYGITTSAL"
     gene            complement(2926..3585)
                     /locus_tag="PKW60_11475"
     CDS             complement(2926..3585)
                     /locus_tag="PKW60_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00098.1"
                     /translation="MADVNLQLVREFFELNMFAVLTNWQRESLRQRVPDSSLQVFVEN
                     VNPVMPHELSFVLNPAGIGAVERALVEVRAWHADKFYPSVIESSPVLWQFVSQESLAF
                     AREIFGSRPFKSILVISELPASPEPRARSIQLLQETGIDHVLEFSSVLRDLVDTVSMH
                     ENYMSSPTLQVLRLMKRYKLVRDQQLEFAFPNEPPAAGPLPHVEIAAERDAETGDGAG
                     L"
BASE COUNT          694 a         1126 c         1211 g          731 t
ORIGIN      
        1 acgccatgcg cgcgcgcctt cgcgaacgcg gggtgcaact gcattcggtc agcttgcggc
       61 agcagaatct ggagaacgta ttccttcggg tcacgggcga tccggcgtcg taaaaggacg
      121 ggaccatggg ttttttgctg gtactcaagg ccgagatagt gcgcggattg atcatcatgc
      181 gccgctactg gtttgccacg atcaccggcc tgatcatcag ttacggattt ctgatgctgg
      241 tcattctggc ctttgtgtcg aaccaggcgg ccctggcgga gtggtcggag agtattttcg
      301 acaaagtggt gggctttctc gtgggcatgc tggcgatggg gctggtgggg atgttcagcc
      361 agggtctcca gggcatggcg cgaaccggcg aactggaaca ggtctgcatg agcccgcacg
      421 ggctggtgac aaatttcctg gcgcgctccc tcgtcgcggc ggtgatgagc attctcacct
      481 acacgattct gctcagcctg ctggcggcga gtctcaaagg caagatctac ctcgagcccg
      541 tgtcgacggc gctggtgctt gccctgacgt acaccaacct gatcgggttt ggctttatgg
      601 ttggaggcct ggtgctggtg ttcaagcaga ccggccagat cgcgatgatt attcggctgg
      661 tgctgctgat cattgcgatt ggatccgagg aagtggccaa attgcccgcc tggtttcgcg
      721 ccattgccca ctgcgtgccg atcacggacg cagcgatctc gttgaaatac gtgctgatca
      781 aaggccagca catcgatggc cggtacagca gcattctggt tcaaccgtcg ttttattacc
      841 tgatcctgaa cgcggccgcc tggaccctga tcggcatcgc gtgtttccgg tatcttgaga
      901 actggagccg ggataaaggc accctggggg cttactgatg cacggcgcag cgctgcaccg
      961 attcgaactc aacggcaaac ggtacgccat cgatcctgag acctgttttt gctttgaatg
     1021 cgacgagatc tgctgggatg tgctcgcgct gtatccgcac gaaaccatca gccgcattgt
     1081 gcatgagttg aagcaaaaac actccgaacg ggaagtgtac gaggttgtag gcgagctgga
     1141 gtggctgcgc aatgtcaaat cgatcctgcc ccggccgact cccgaagaac tgcagaaacg
     1201 ctacgaaata gagcggggac tgaagcggct gacggtcgaa cttcccgaag aaacctccgt
     1261 ggcggcgccc gcgccggaaa aaacgggatg gtttcgcaaa cgccgtgaag tgtcttcagc
     1321 ggggctgtct tcccttgggc gagatgcggc ccatctgctc ctcaaccgtt ccggcaacca
     1381 gaaggtgctg gaactcgaat ttctggaaca cgggtgcatc catgaccttg atgccgtggc
     1441 agcgttgtgc gccgatgtcc tgcgcgcggc ccgcctggcc ggcaaagagc tcacggcggc
     1501 cgtacggatc caggatctgc gtctggagcc agagcccggc gccctgaaag ggcacaccgt
     1561 gagcgcgcgt ctggagctta ccgaaccgga tgcggcgcag agcgcgcttg cgccgctggc
     1621 cgccggtaac gtgttcaccc tggcgcagct cgcgaaagcg cttcagccgg gggcaagcgg
     1681 cgtccagggc cggatcatcg tgcggccgga tacgcccgat tttggcggcg tggttcagac
     1741 gctcgacgaa gccggcttca aagtgattga gctcgacctc gatgcgacct ttgttgcgca
     1801 tccggacctg ccccctgccg aggtgctgcc tggattaagc gcgagcgcga tctactatgc
     1861 gcgccgcctg ctcgaaggcc attattttcg tctggacccg atagcctcgc tgttttaccg
     1921 catctatgag ggccagccga tccagcgcgc cgaccccatc ggcaccaacg agctggccgt
     1981 cgacagccgc ggcgatatat acgcctcgtg gcgggtgttc gggaacgagc ggtttcgcca
     2041 cggctcactg cagaacgcca cgatcgacga agcggccatc aaacgctacg atgacatggg
     2101 cgtgcccaca acgggaccgt gcctgcgctg ctgggtccgg catctgtgcg gcggcgggcg
     2161 cgcggccgtg cacgatgccc tgagcggcaa tttccatcag ccgcacgagc cctggtgcga
     2221 cgcgcagcgc tcatggatgg ccgccgcggt ctccgcattc aacgtgcttg cggcggaggg
     2281 gatcaatttc acacgcatct accagacgct cacgccctcc gcgggaaagc gccccacgct
     2341 gtcgatgctc acgatgctgc gggcagcgat tggcctgtcg attcgcatgc gtcccgtgga
     2401 agaatccgat gcggaacttc tggtgcgctg ggaaaactgg aacccggcgg cttattttct
     2461 tttcaatgag aaaggggcgt ttgtcgcgac ccgctatgaa cgcgagatgg acgcgcttca
     2521 tccgcagacc ttcgaccagg aactggtgct gacgcgcaaa gacgggacgc cgttcgggct
     2581 gctcaagctt cgtgcggaaa aggtggctga gaccgcgtcg gcgtggctct atttccgcaa
     2641 cgaagccgat tatgccgcgg ctgacctgcg caagggactg gcgttcctgt taaaacaagc
     2701 gggccagcag caggggttgc ggcgcatcac cgtctccacg gccgagtatg aacactccct
     2761 gcagcacttt ctggaacagt gcggcttcac gcgtgagggc acacttcgtg aagcactcta
     2821 cctgcacggg acctaccgcg acgtgcacgt ttacgggatt acaacgtcag cgctgtaaac
     2881 cggctggatg ggcggctccc tcgcatccgc ccgggcagca cctcctcaga gcccggcgcc
     2941 gtccccggtt tccgcgtcgc gttcggcggc gatctcgaca tgcgggagag ggcccgcggc
     3001 aggcggttcg ttcggaaagg cgaattccag ttgctggtcg cgcacgagct tataccgttt
     3061 catgaggcgc aggacctgca gcgtgggaga cgacatgtag ttttcgtgca tgctgaccgt
     3121 atccaccagg tcgcgcagga cgctggaaaa ctcgagcacg tgatcgatgc ccgtctcctg
     3181 gagcagctgg atggagcgcg cccggggttc cggtgacgcg ggcagttccg agatgaccag
     3241 aatgctcttg aaggggcggc tgccgaatat ttcgcgcgca aaggccagcg attcctggga
     3301 tacgaactgc cagagcaccg ggctggattc gatgaccgag gggtaaaatt tgtcggcatg
     3361 ccaggcgcgg acctcaacca gggcgcgctc cacggcgccg atcccggcgg gattcaggac
     3421 gaaactgagc tcgtgcggca tgacgggatt cacgttttcg acgaagacct ggaggctgct
     3481 gtcgggcacc cgctggcgca ggctctcgcg ctgccagttg gtcagcacgg cgaacatgtt
     3541 gagttcgaag aactcccgta ccagttggag gtttacatcg gccatgacag ttcccgttcc
     3601 agcgcccggg gccgggacac cggcgtgctg cctcagggcg cgctcagcgc cttgccaatc
     3661 ttgtgttccg gatcgagcgc caggatttcc gccccgagtt ccctggcccg gtccaaacgg
     3721 tccagttgcc ggtagcacaa cccgagtttg gccaggatgt cg
//
LOCUS       DAOPBQ010000378         3747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_19888_length_3747_cov_3.501083, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000378 DAOPBQ010000000
VERSION     DAOPBQ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19888_length_3747_cov_3.501083"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PKW60_11480"
     CDS             <1..1023
                     /locus_tag="PKW60_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00099.1"
                     /translation="IRHTNPCPPDDEVAGVGPMDPEIGVLRIDRTDGRPFAVVYNFAC
                     HPYVTVPRGGVTADFPGFASAVIEENLGGGAMALFLQGAGGDITEILYKDVHRPRDSE
                     PLGTLLGLSTLEALRDIKTGAGKLKVVNEPLELPRRTDFDERVAELEAEQAQLLRSLR
                     GTSLNIKTFVPLYIQYQLDPEHPSYYSYRYLQSDLLGTDELSEIDAQNRAHLDKYLRN
                     CRAMEKMARIQDKIATLRKHQEFNLASGESTIATEVQGIRIGPFVLITTATELGVEIG
                     LNLKKASPFEYTFVAAYSNGYLQYGPPAADYPKGGYEVTECFLAPEWQAIYEAKALEV
                     LNKLEF"
     gene            1133..2593
                     /locus_tag="PKW60_11485"
     CDS             1133..2593
                     /locus_tag="PKW60_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPK00100.1"
                     /translation="MIHRREFLRQCGLGALALACAGAAPAAAKPRRPNVVFILVDDLG
                     WYDLSCYGSTFHETPRLDRFAASGMRFTDAYASCPVCSPTRAAIMTGKHPARLNITDW
                     IPGDDPKDRKLLGPQDRHELPLEEVTLAEVLRAAGYRTFFAGKWHLGGAGFYPEDQGF
                     EINLGGCEKGQPPGGYYSPYKNPKLSDGPEGEYLTDRLTDESIRFLESAKDDPFLLYL
                     SFYTVHTPIQACTRHLAHFTEKAAALPEPEGPVFLEVPGGTTRMRQDRPDYASMVRAM
                     DENVGRLLDALDTLGLADNTIVIFTSDNGGLTTDGGYDAPTSSGPLRAGKGFCYEGGI
                     RVPQIIRVPGRTAAGSVCDVPVASADFYPTLLELSGIALPDDEPRDGMSFASLLGGGP
                     SPEREALFWHYPHYHGACWRPGAAVRAGDWKLVEFYEDERAELYNLRDDLGEQHDLSA
                     AMPEKTRELREKLHAWQRRVGAQMPVPNPAHTGGES"
     gene            2768..>3747
                     /locus_tag="PKW60_11490"
     CDS             2768..>3747
                     /locus_tag="PKW60_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPK00101.1"
                     /translation="MHRTSAPFRMTLGLCLALLLGTVAVCAAAQNDHGPTERVAPPSE
                     AKMARHNAMKERLLEGNADLLFIGDSITQGWESAGKEVWDYYYADRNAVNLGTSGDRT
                     YHVLWRLDDLDFSRVSPKLAVIMIGTNNHGIHSAEQIAEGVTAIVQTVRERLPQTKVL
                     LLGIFPREAAPDAENRVKLAAANQILAGLNDGQNVFFLDIGEEFLNEDGVLPESIMPD
                     ALHPNTEGYWIWARAMEPAVARLMGTMSPDNPPKGYVALFNGKDLTGWKGLLKGPLDN
                     PEQRAALTPDELAANLADFWAFASRRKGRGPMMRLVPMLGEGAAGLDRLE"
BASE COUNT          742 a         1058 c         1267 g          680 t
ORIGIN      
        1 attcggcaca cgaacccctg cccgcccgat gacgaggtgg caggcgtcgg gcccatggac
       61 ccggagatcg gcgtactccg gattgatcgg acggacgggc ggccttttgc ggtcgtctac
      121 aattttgcat gccatccgta tgtgaccgtg ccccgtggcg gggtaacggc ggattttccc
      181 gggttcgcgt cggccgtgat tgaagagaat ctgggcggcg gggcaatggc gctgtttctg
      241 cagggagcgg ggggcgacat cacggagatt ctgtataagg atgttcaccg gccgcgggat
      301 tccgaaccgc tggggacgct gctcggattg agcacgcttg aggcgttgcg ggacatcaag
      361 accggggccg ggaaactgaa agtggttaac gagccgcttg agctgccccg gcgcaccgat
      421 ttcgacgaac gggtcgcgga actggaagcg gaacaggcgc agctgttgag atcgctgcgc
      481 ggcaccagct tgaatatcaa gacattcgtt cccctgtata tccaatatca gctggacccg
      541 gagcacccgt cctattattc gtaccgctac ctgcaatcag acctgctggg cacggatgaa
      601 ttgtcggaaa ttgacgcaca gaaccgcgca cacctggaca aatacctgcg gaattgccgg
      661 gcgatggaga aaatggcgcg gatccaggac aagatcgcta cgcttcgcaa gcaccaggaa
      721 tttaatctcg cctcgggcga atcgaccatt gccacggagg tacaggggat ccggatcggg
      781 ccgttcgtgc tgatcacgac ggcgacggag ctgggcgttg aaatcgggct gaatctgaaa
      841 aaggcttcac cgtttgaata cacctttgtc gccgcgtatt ccaacggcta cctccagtat
      901 gggcctccgg cagccgatta tcccaagggc ggatacgagg tgacggaatg cttcctggcg
      961 ccggaatggc aggcgatcta tgaagcaaaa gctctggaag tcctcaataa actcgaattc
     1021 tgaggctttt ccgggccaaa tctgaaaaaa ggggagcagg gggcctgttt ctggcgtgtt
     1081 tcgcgtcgtg ggcgcgggtt cccgctcgaa cgcggaggtt ttcgaggcgg ccatgataca
     1141 tcggcgcgaa tttctgagac agtgcggtct gggtgcgctg gcgctggcct gtgcgggagc
     1201 ggccccggca gcggcaaagc cacgccggcc caacgttgtt ttcatcctcg tggatgactt
     1261 gggctggtat gacctgagct gctacggcag cacctttcac gaaacgcccc gcctggatcg
     1321 cttcgcggcg tccggcatgc ggttcactga cgcgtacgcg tcgtgcccgg tgtgttcgcc
     1381 gacacgtgca gccattatga cggggaagca tcctgcacgg ctgaatataa ccgactggat
     1441 tcccggggac gatccgaagg acaggaagct cctgggtcca caggaccggc acgagctgcc
     1501 gctcgaggaa gtcaccctgg cggaggtgct gcgtgccgcg ggctaccgga cctttttcgc
     1561 cggcaaatgg catctgggcg gggccgggtt ctacccggaa gaccagggct tcgagatcaa
     1621 tctcggcgga tgtgagaaag gccagccacc gggcggctat tactccccgt acaaaaaccc
     1681 gaaactcagc gacgggccgg agggcgaata tctgacggat cgcctgactg atgaaagcat
     1741 ccggttcctc gaatcggcca aagacgatcc atttctattg tatttatcct tttacacagt
     1801 gcataccccg attcaggcct gcacgcggca cctggcgcat tttacagaga aggcggccgc
     1861 cctgcctgaa ccggagggcc cggtgtttct cgaggtgccc ggcggcacga cccgaatgcg
     1921 gcaggatcgc ccggattatg cgtcgatggt gcgggcgatg gacgaaaatg tgggacggct
     1981 cctggatgcg ctggatacgc tcgggctcgc ggacaatacg atcgtgatat tcacctcgga
     2041 taacggcggg ctcacgacgg acgggggtta cgatgcgcca acctcctcgg ggccgcttcg
     2101 tgcgggcaag ggtttttgtt acgaaggggg gatccgcgtg ccacagatca tccgcgtgcc
     2161 gggcaggacg gcggcgggga gcgtctgtga tgtgccggtg gcgagcgccg atttttaccc
     2221 cacgctgctg gaactgtcgg ggattgcctt gccggacgac gagccgcgcg atggaatgag
     2281 cttcgcgagc ctgcttggag ggggtccttc gccggagcgc gaggctcttt tctggcacta
     2341 tcctcattac cacggtgcgt gttggcggcc cggtgcggcg gttcgcgccg gcgactggaa
     2401 actggtcgag ttttatgagg acgagcgggc cgagctgtat aacctccggg atgatctggg
     2461 agaacagcac gacctctccg cggcgatgcc cgagaagacc cgtgaactgc gcgagaaact
     2521 gcatgcctgg cagcgtcgcg ttggcgcgca gatgcccgta cccaatccag cgcacaccgg
     2581 cggggaatcc tgaccttttc ccgtgacaaa gaccgtattg gcgcccatga ttccaggtga
     2641 gaagcaggag ctgcgcgccc ggcaatcgag aatgtgcttg cacgaagagt gcgcaggggg
     2701 cacaataaga cgctctgggg atcgttggaa cggatccctg tcccaccacg aacgagaggt
     2761 acagactatg catcgaacaa gtgcaccatt tcgaatgacg ctggggttgt gcctggcgct
     2821 gctgcttgga acggtagcgg tgtgtgctgc cgcgcagaac gatcacggcc cgacagagcg
     2881 ggtggcgccg cccagcgagg ccaagatggc gcggcataac gccatgaaag agcgcctgct
     2941 tgaaggcaac gcggatctgc tgttcatcgg agactcgatc acgcagggct gggagagcgc
     3001 ggggaaagaa gtctgggatt actactacgc cgaccggaac gcggtgaacc tgggcacctc
     3061 cggcgatcgc acgtatcacg tgttgtggcg cctcgacgac ctggatttca gcagggtgtc
     3121 gccgaaactg gccgtgatca tgatcgggac gaacaaccac gggatacact ccgccgagca
     3181 gatcgcggaa ggcgtgacgg cgatcgtcca gaccgtgcgg gagaggctgc cgcagaccaa
     3241 agtcttgctg ctggggatct tcccgcgaga ggccgcgccg gatgccgaaa accgggtgaa
     3301 actggccgca gcgaaccaga tcctggcggg cctgaatgac ggccagaatg tctttttcct
     3361 ggatattggc gaagagtttc tgaatgagga cggcgtgctg ccggaatcga tcatgccgga
     3421 cgcgcttcac ccgaacacgg agggatattg gatctgggca cgggccatgg agccggccgt
     3481 ggcgcggctg atgggcacga tgtcgccgga caacccgcca aaagggtatg tggcgctgtt
     3541 taatggaaaa gatttgacgg ggtggaaggg actgctgaaa gggcccctgg acaaccccga
     3601 gcagcgggcg gcgctgacgc ccgacgagct ggccgccaac ctcgccgact tctgggcctt
     3661 cgcctcgcgg cgaaagggcc gtgggccgat gatgcggctg gttcccatgc tgggcgaggg
     3721 cgcggcgggc ctggaccggc tcgagat
//
LOCUS       DAOPBQ010000379         3740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_19935_length_3740_cov_3.345726, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000379 DAOPBQ010000000
VERSION     DAOPBQ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19935_length_3740_cov_3.345726"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKW60_11495"
     CDS             <1..221
                     /locus_tag="PKW60_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HPK00102.1"
                     /translation="DALAVGISFSIVAVGIWYPAAIIGIITGMLTTAGMVFGARLGLR
                     SGRCIELTGGGILVAIGVKILLEHLLAG"
     gene            311..1096
                     /gene="nagB"
                     /locus_tag="PKW60_11500"
     CDS             311..1096
                     /gene="nagB"
                     /locus_tag="PKW60_11500"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPK00103.1"
                     /translation="MEVIIRATQEEAVSLTAALIAQALVQKPNLVLGLATGRTMESVY
                     ELLAGMHRDNDLDFSLARTFNLDEYVGLPPEDPNSYRFYMNKHLFHRVNIDLRNTHLP
                     NGMAEDIAAECRHYEEEITDCNGIDLQLLGIGRTGHIGFNEPMSALHSRTRPKALAPG
                     TVAQNEPLFTEGTAMPRRAITMGVGTILESRRCILLATGKEKAEIIAKAVEGPVTAMI
                     SASALQFHPNCTVIVDQGAGSLLQNTDYYRWVFENEPEWEPYQ"
     gene            1228..2160
                     /locus_tag="PKW60_11505"
     CDS             1228..2160
                     /locus_tag="PKW60_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK00104.1"
                     /translation="MSHIMLVDDDVDLAQLIRTKLVAEGNEVSVIHSGDGAFEMAKQV
                     KPDLIILDIMLPGETGYRICRRLRKDPELYTVGILILTALGEEPEVLHGLEQGADDYL
                     SKPFKLDHLMDKIASLEALLESSRQRHTSTGLPGTEAIKREINHRLARNMALACCYID
                     VVGYKPYCANTGASGQARALEFTARLLTTLTRDMGVYESYTAHLGGEHFVVMLNLEDY
                     ERFCNTLMEMFDQKVRDLYTPEQVQKGYITATDRRGKTGRYPLMMLSIGVAHNQYRQY
                     KSAKKMFEVLAQTRQMAKPTNKSVMFVDRRQADR"
     gene            2168..2647
                     /locus_tag="PKW60_11510"
     CDS             2168..2647
                     /locus_tag="PKW60_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00105.1"
                     /translation="MPSRRLPVAALAVAGVVVLVMILSAKGPMSSEELARRVMKCEPS
                     WSNYDEDLKAQIGAAPVAEWKGEPAEARWEAGELRVSFAMLGVWAGRDAAIPVLVKLP
                     NGETHRNEAAAWEDGRLVYQFTTSLQATPQWVIVRYPFYGERRLVLSEDGRWESAEH"
     gene            2739..3641
                     /locus_tag="PKW60_11515"
     CDS             2739..3641
                     /locus_tag="PKW60_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HPK00106.1"
                     /translation="MPVEMTPGSPELTPELRRQLLAMQRNEITEYHVYRRLAQRQSSI
                     HNRDTLLRIAEDERTHHDFWRGYTRESTKPSRLRIVLYASIARVLGLTFAVKLMERGE
                     EAAQVSYEAIAAIIPEAKYVLEEEERHERQLLDILDEERLRYIGSIVLGLSDALVELT
                     GALAGLTLALGNSRLIALIGLITGIAAAMSMAASEYLSRRAEEKKDHSPLKAAVYTGI
                     AYIATVTLLVMSFLLLNNPLVSLAITLLFALLIIVSFTYYLAVVRDMPFKHRFLEMAG
                     LCLGVSLISFGIGFVLRQAFGIDL"
BASE COUNT          768 a         1062 c         1178 g          732 t
ORIGIN      
        1 tcgacgcgct cgcggtgggg atcagtttca gcatcgtcgc cgtcggcatc tggtatcctg
       61 cggcgataat tggcattata accggaatgc tgaccaccgc aggcatggtg ttcggcgcgc
      121 ggctggggtt gcgttccggc cgatgcatcg aactcaccgg tggaggtata ctagtcgcca
      181 taggcgtcaa aatcctgctc gagcatctcc tcgcaggatg attcagtgcg gggacagggg
      241 gactgtcctg gtccgagaag cgggcggcgc gaacgggaag aacaatcggc acggaagaaa
      301 ggttgtttca atggaagtga ttattcgagc cacgcaggaa gaagcagtat ccctgacggc
      361 ggccctcatt gcgcaggcgc tggtgcagaa gcccaatctg gtgctgggac tggccacggg
      421 ccgcacgatg gagtcggtat acgagctgct cgcaggcatg cacagggata acgatttgga
      481 tttctcgctg gcccggacat tcaatctgga cgaatacgtg gggctgcctc cggaggaccc
      541 caactcctac cggttttata tgaacaagca cttgtttcac cgcgtgaaca tcgatctgcg
      601 gaacacccat ctgcccaacg ggatggccga agatatcgca gcggaatgcc ggcattatga
      661 ggaagagatt acggactgca acggcatcga tctgcaattg ctcggtatcg gccgcacggg
      721 acatatcggt tttaacgagc ccatgtccgc tctgcattcg cgcacccggc ccaaggcgct
      781 ggcgcctgga accgtggcgc agaatgagcc gctcttcacg gagggcacgg cgatgccacg
      841 ccgcgcgatc accatgggcg taggcaccat cctggagagc cggcgatgca tcctgttggc
      901 cacgggcaag gaaaaagctg agataatcgc caaggccgtg gaggggcccg ttaccgccat
      961 gatttccgca agcgccctgc agtttcaccc caattgcacc gtcattgtgg accagggggc
     1021 gggttcgctg ctgcagaata ccgattatta ccgctgggtg ttcgagaacg agccggaatg
     1081 ggagccgtac cagtagcagg cagcgggttc cctggccact gctcccgggc cgggaacatg
     1141 gcccggagcg ggatgttgcg ttcacaattt gtggcatact atgattcagc agctctgcgg
     1201 ccggaatgcg aaatgaaagg gtgactcatg agtcatatca tgctggtcga tgacgacgtt
     1261 gatctcgctc aactcattcg caccaaactg gttgcggaag ggaacgaggt ttccgtcatc
     1321 cattcgggcg atggcgcgtt tgaaatggcc aagcaagtta aaccggacct gatcatcctc
     1381 gacatcatgt tgccgggcga gacggggtac cgcatctgca gacgcctgcg caaagatccg
     1441 gagctgtaca ccgtcggtat tttgattctg acggcgctgg gcgaagagcc ggaagtgctg
     1501 cacgggctgg agcagggcgc cgacgactat ttgtccaaac ccttcaagct tgatcacctc
     1561 atggacaaga tcgcttcact ggaagccctg ctcgaatcgt cgcgccagcg gcacacgtcc
     1621 acagggttgc cgggcacgga ggccatcaag cgagagatca accaccggct ggcacgaaac
     1681 atggcgctgg cctgctgcta tatcgacgtg gtgggttaca aaccgtactg cgccaatacc
     1741 ggtgcgagcg gccaggcccg tgcgctggaa ttcaccgccc gcctgcttac tacgctcacc
     1801 cgggatatgg gtgtgtacga aagctatacg gcacatctcg gtggcgagca tttcgtggtc
     1861 atgctgaatc tggaagacta cgaacggttc tgcaatacgc tcatggagat gtttgaccag
     1921 aaagttcgag acctctatac cccggagcag gtgcagaagg gatatatcac cgctaccgac
     1981 cgtcgcggga aaaccgggcg gtatccgctg atgatgctgt cgattggcgt ggcccataat
     2041 cagtaccggc aatataaaag cgccaaaaag atgttcgaag tcctggcgca aacccgccag
     2101 atggccaaac cgaccaacaa aagtgtgatg tttgttgacc gccggcaggc cgaccggtag
     2161 gcctccgatg ccgtcgcggc gcctccccgt agctgcactg gctgttgccg gtgtggtcgt
     2221 gctggtgatg attctctcgg cgaaggggcc catgtccagc gaggaacttg cgcgccgcgt
     2281 catgaaatgc gaaccgtcct ggagcaatta cgacgaagac ctgaaggcgc aaatcggagc
     2341 cgcccccgtc gcggaatgga aaggcgaacc cgccgaggct cggtgggagg caggcgaact
     2401 gcgagtttct ttcgccatgc tgggcgtatg ggccggacgc gatgccgcca tacccgttct
     2461 ggtgaaactg cccaatggcg agacgcatcg gaacgaggct gcagcctggg aggacgggag
     2521 acttgtttac cagttcacga cgtctctgca ggccacgcca caatgggtga tcgtgcggta
     2581 tcccttttac ggggagcggc gccttgtgct gtccgaagac ggccggtggg agagtgcgga
     2641 gcattaacca acgccggtgc cgtggagatt cccggctcgt ctgaaactgc cattttcggc
     2701 ggggccatgc gcgaaaacca cagcaggagg cttcagccat gcctgtagag atgactcccg
     2761 gttcaccgga gctcacgccg gagctgcgac gccagctcct ggccatgcag cggaatgaaa
     2821 ttacggagta ccacgtctac cgccgcctgg cccaacgcca gtcgagcatc cacaatcgcg
     2881 acaccctgct gcggatagct gaggatgaac gcacgcacca cgacttctgg cggggatata
     2941 cgagggagtc cacaaagccg agccgcctgc gcatcgtttt gtatgcctcc atagcgcggg
     3001 tgctgggcct gaccttcgcc gtcaagctga tggaacgggg cgaggaagcc gcgcaggtca
     3061 gctacgaggc gatcgcggcc atcatccctg aggcaaaata cgtgctcgag gaggaagaga
     3121 ggcacgaacg gcaattgctg gacattctcg acgaggagcg gctgcggtat atcggctcca
     3181 tcgtgctggg actgagcgac gcactggtcg agttgacggg tgctctggcc gggctgacgc
     3241 tggcgctggg caacagccgc ctcattgccc tgatcggatt gatcaccggc attgccgccg
     3301 cgatgtccat ggccgcctcg gagtacctgt cgaggagggc cgaggaaaag aaagaccact
     3361 cgccgttgaa ggcggcggtc tacacgggga tcgcgtatat cgcaacggtc acgctgcttg
     3421 tgatgtcttt tctgctgctg aacaatccct tggtttctct ggcgatcacc ttgcttttcg
     3481 ctctcttgat catcgtgtcg ttcacgtatt acctggccgt ggttcgtgat atgccattca
     3541 agcaccgttt cctggaaatg gccgggctct gcctgggggt atctcttatc agcttcggaa
     3601 tcggcttcgt cttgcggcag gcctttggga tcgacctgta gagggaacgg ttccgtgcgg
     3661 atgggcccgg gagggcggct cgccgcctgg ctgcaagatt cctgaccgct atttcggtac
     3721 gctttccctc tctgccgatg
//
LOCUS       DAOPBQ010000380         3739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_19940_length_3739_cov_3.831705, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000380 DAOPBQ010000000
VERSION     DAOPBQ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19940_length_3739_cov_3.831705"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="PKW60_11520"
     CDS             <1..805
                     /locus_tag="PKW60_11520"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPK00107.1"
                     /translation="AEMAACDRRTIEGGVPGGLMAAYGIEEWLGEDGMYPERMLRFLK
                     ALGPDLWDTMEPACAIDCYLKHPAFTDAFPEKIMKRLQYRETLDGLEVYANEAAFPRA
                     YLAPDVRVCPDRETMYSLMADPGFDPARTALLEAPLATPLPGTGQGTATVASHGFTEV
                     VVETEADDAALLILADAYYPGWTATVNGTPAAIVPVYSVFRGVAVPEGRAEVRFTYFP
                     ATFRVGLACSVLSLAAGSIAGLLSLRRIRPRASQTPMNGAVELSRPEAP"
     gene            897..>3739
                     /locus_tag="PKW60_11525"
     CDS             897..>3739
                     /locus_tag="PKW60_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPK00108.1"
                     /translation="MRYVKPHSTLFSWSLVILLSAVMLTCAGCGQPAPPQPAPQPGET
                     ARATAPTPQESLQPEPSALALAGVLPLAGEALDKQIVFFFTEPAQVAPEAGYSEPFTI
                     DPPVQGVFRISGNFVAFRAADGFAENTLYTVDLNPDLVSASGARIPEVARRHRFATFI
                     FEPQQVWEIEDQPDRVVLGILFPTTVNFDSLREHLSVESQQDQPVEYALESGEDGVVR
                     LVFAEGLAEPIVIKISKGVEDETGTTELETDHTFTYPEEPFFSVTSIQWGQFEGPKKE
                     IVLQFTKPVAAESLATGVSIARTDNQSAVPFEIASRGTRTEHRIQVDLSDGDFPSLTV
                     QLAKDMPGSQGRHLVEGYEATLATRPDPLTVASVRWSANRDWNEQLFLKFSHPVRNEA
                     LADRLKLTDTATGDNLTFSIQGEALSVDKTVQFEPVTRDMESVTLAIEAGLPGEPNAV
                     LNEPYQYEATRPVEPLRLEDTWWGEDSREGLFLTMRLNAAVSSQELEKHLAFTPAVED
                     IRIVPTGNNYFRILGTFRSKTAYQLHIAEGVTYAGGAKSQEALTRELQTEQVPSYVGF
                     NHDGKFYFPTRATSALAVKSRNVEKVSVEIYRMFPSNVAVAVSDMGMSASQDDYWESQ
                     RFGSNFIHKWSEKLGSSELEMPTQQDVVVSAALNLEGLLPSDKRGVFCVQARSDRGHS
                     ASKIVLFTDIGVMAHWQNNELMLFAHNLFSLEPLAGAKVTLHSNKNQLLAIGHTNTQG
                     ILHMSNLDTNLGNPAVAVVEHGDDCTLIELKRSNDATPEILPGMPGYDRDGYDGFIYA
                     DRDLYRPGETVHARWIVRTNYGDALAETPLLLKVLKPNGRELMSRITNLTAFGSGELD
                     IETQKEYPTGKYTIALEVPGSKTPVGTYQFSLEEFVPNRIETKVELPLTVWLAGQSYE
                     IHVEARHLFGAPAVNRMSNAKVALERKGWSPAG"
BASE COUNT          800 a         1088 c         1126 g          725 t
ORIGIN      
        1 agcggagatg gcggcgtgcg accgccgcac gatcgagggc ggtgtgcccg gcgggttgat
       61 ggcagcgtac gggattgagg agtggctggg tgaagacggc atgtaccccg agcgcatgct
      121 tcggtttctc aaagccctcg ggcccgatct atgggacacc atggaacccg cctgcgcgat
      181 cgattgctat ctgaaacacc cggcctttac ggacgccttt cccgaaaaaa tcatgaaacg
      241 tctgcaatac cgggagactt tggacggtct ggaggtgtat gcgaatgagg ccgcgtttcc
      301 ccgggcttat ctggccccgg acgtgcgcgt atgtccggat cgggagacga tgtactcctt
      361 gatggcagat cccgggtttg atccagcccg cacggccctg ctcgaggcgc cgcttgcgac
      421 gcctttgcct ggcaccggcc agggaactgc aaccgtggca agccacggct tcaccgaagt
      481 cgttgtagag accgaagcag acgatgccgc gctgctgatc ctggccgatg cgtattatcc
      541 cggctggacc gcaacggtca acggtacacc tgcggctatc gtgccggttt actccgtttt
      601 tcgcggggtg gccgttcctg aagggcgtgc tgaagtacga ttcacctatt ttccagccac
      661 cttccgggtt ggtttggcct gcagtgtgct ctccctggca gcggggagta tcgccgggct
      721 gctttcactg cgacgtatcc gcccgcgcgc gagccaaacc ccgatgaatg gcgcggtgga
      781 actttcacgg ccggaagccc cataaataga gtgagaaatt gcggcgactc gcgcataatg
      841 gtagcgcgtg ttgcccaagg cgaagaccgt tcgataactt gcgggagagc cccctcatgc
      901 ggtacgtcaa gccccactcc acccttttct cctggtcgct cgtgatcctc ctgagtgccg
      961 tgatgctgac ctgcgccggc tgcggacagc cggcgccgcc ccagcccgct ccgcagcccg
     1021 gggaaaccgc ccgcgccaca gcaccgacac cgcaggagag cttgcaaccc gagccatccg
     1081 ccctggcact cgcgggcgtt cttcctctcg cgggagaggc cctcgacaag cagatcgtgt
     1141 tctttttcac cgagccggcg caggtcgcgc ccgaggccgg atacagcgaa ccgttcacga
     1201 tagatccgcc cgttcaggga gtctttcgga tcagcggcaa tttcgtcgct ttccgggctg
     1261 cggatggctt cgcagaaaat actctctata ccgttgactt gaatcccgac ctggtgtcgg
     1321 catccggagc gagaatcccg gaggtcgctc gccggcaccg tttcgcgaca tttatttttg
     1381 aaccgcagca ggtgtgggaa attgaagatc aaccggaccg ggtcgtgctc ggcatattgt
     1441 ttccgacgac cgtgaatttt gattcattgc gcgaacacct gtccgtcgaa agtcagcaag
     1501 accaaccggt tgaatatgcg ctcgaatctg gagaagacgg agtcgtacgg cttgtgtttg
     1561 ccgagggtct ggccgaaccc attgtcatca agatcagcaa aggggtggaa gacgaaacgg
     1621 gaaccacaga actcgagacg gaccatacgt tcacctaccc ggaggagccg ttcttttcgg
     1681 tgacgtccat tcaatggggc cagttcgaag gaccgaagaa ggaaatcgtg cttcagttta
     1741 cgaaacccgt cgcggcagaa tctctggcca caggcgtttc gattgcgcgc acggacaacc
     1801 agagtgcggt cccgttcgag attgcgtcgc ggggcacacg cactgaacat cgcattcagg
     1861 tggatttgag tgatggcgac tttccatcac tgaccgtgca actggcgaaa gacatgcccg
     1921 gcagccaggg ccggcatctc gttgagggct atgaggccac gctcgccacg cggcccgacc
     1981 cccttaccgt ggcctctgtg cgctggagcg cgaacagaga ctggaacgaa caattgtttc
     2041 taaaattttc ccatccagtc cgcaacgaag ccctggccga ccgcttgaaa ctaaccgata
     2101 ctgccaccgg cgacaatctg acattttcaa tacaagggga agccctgtcc gtagacaaga
     2161 ccgtgcagtt tgaacctgta acgcgcgaca tggagagtgt gacactcgca attgaagcag
     2221 ggttgccggg cgagcccaat gcggtgctga atgaaccgta ccaatatgag gctacccggc
     2281 ccgtagaacc cctgcgcctt gaagacacct ggtggggcga ggattccagg gagggcctgt
     2341 ttcttacgat gcgcttgaat gcagcggtaa gctcgcaaga actcgagaag cacctcgcat
     2401 tcacgccggc ggtagaagac atccgaatcg taccgacagg caacaattat ttccgaatat
     2461 tggggacatt ccgctcaaaa acggcatatc agcttcacat cgcggaaggc gttacttacg
     2521 ctggcggcgc caagagccag gaggcactga cgcgagagct gcagacagaa caggtcccct
     2581 cgtatgtggg attcaatcac gacggaaaat tctacttccc gacccgggcc acgtcggcgc
     2641 tggcggtcaa gtcgcgcaat gtcgagaaag tgtcggtgga gatctaccgg atgtttccgt
     2701 cgaacgtcgc ggtagcggtc agcgacatgg gtatgtcggc atcgcaggat gattactggg
     2761 aatctcagcg gtttggcagc aatttcattc acaagtggag cgagaagctg ggttccagcg
     2821 agctcgaaat gccaacccag caagacgtcg ttgtcagcgc agcactgaac ctggagggac
     2881 tcctgccgag cgacaagcgt ggggtcttct gcgtccaggc gcgttccgac agaggccata
     2941 gcgcttcgaa aatcgtgctg tttacggata ttggcgtgat ggcgcactgg cagaataatg
     3001 aactgatgct tttcgcacac aacctgtttt ctctggaacc tctggccggc gccaaggtaa
     3061 cgctccattc caataaaaac cagttgctgg ccattggcca caccaacacg cagggcatcc
     3121 tgcacatgtc caacctggat acgaatctgg gaaaccccgc agtggccgtt gtggaacacg
     3181 gcgacgactg caccctgatc gaactgaaac gcagcaacga cgctacgcct gaaatcctcc
     3241 ccggcatgcc gggatacgac cgggatggct acgatggttt catctatgcc gatcgcgacc
     3301 tctaccgtcc gggtgaaacc gtgcatgcgc ggtggattgt gcgcacgaat tacggagatg
     3361 ccctcgcgga gacgcccctg ctgctgaagg tgctgaaacc caacgggcgc gagttgatgt
     3421 cacgcatcac caacttgacg gccttcggga gcggcgagct ggatatcgag acgcaaaagg
     3481 aatatccgac ggggaaatat acgattgcgc tggaggttcc gggcagcaaa acgccggtgg
     3541 gcacgtatca attcagcctg gaggaattcg tgcccaaccg cattgaaacg aaggtggagc
     3601 ttccgctgac cgtgtggctg gcgggacagt cctatgagat tcacgtggaa gcgcggcatc
     3661 tcttcggtgc cccggcggtc aaccgcatga gcaacgcgaa agtcgcgctc gagcggaagg
     3721 gctggagccc tgccggttg
//
LOCUS       DAOPBQ010000381         3738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_19948_length_3738_cov_3.233777, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000381 DAOPBQ010000000
VERSION     DAOPBQ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19948_length_3738_cov_3.233777"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(510..908)
                     /locus_tag="PKW60_11530"
     CDS             complement(510..908)
                     /locus_tag="PKW60_11530"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HPK00109.1"
                     /translation="MTAQSRLLNDPLQNAVEIQLDAARVGFDWPDIAPVFDKILEELD
                     EIRDALAHGQPDDVRRELGDLLFAVVNLARFVNADPSRELEATNHRFLARFGKVRDAL
                     EQAGRRIEDCSLEEMDQVWQRVKNAERHTE"
     gene            1424..2404
                     /gene="bamD"
                     /locus_tag="PKW60_11535"
     CDS             1424..2404
                     /gene="bamD"
                     /locus_tag="PKW60_11535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPK00110.1"
                     /translation="MSVVSVRKCGVLLALGLLVVGTTADAWVWNPATGRWINEKNLPQ
                     PTAELQVEAARSLMEQGEYKRAMRETHKFDKYYLDSEWADDNQFLRGEINLRRGDYLK
                     AANEFQQVITNYPDTDLYDTVIAKQYEIGDRFYAQGEKRLGKRWSPFRKRPFKKAIEV
                     YSMVIDNQPFQETAAEAQYKVGLCHFTRNEYVEAAYEYQRVLEDYASSAWVDDASYGL
                     AMCYYKASLPPDYDQTRSRLAIDAIDEFKARFPGDERAEGLDAKRAEMSEKIARQRLD
                     TARFYEKRRRFTAARMYYEVVLEQFPETSAAADAKLWLEQSVQQEQTAQP"
     gene            2401..2964
                     /locus_tag="PKW60_11540"
     CDS             2401..2964
                     /locus_tag="PKW60_11540"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPK00111.1"
                     /translation="MKNRWALAGCVVIMAWAAGCGYSTHSTLDAQYQTIAVPAFRNLS
                     REYDLQAPLTNAVIRKFMADSRLEIVPLDQADLILEGVILGYERKGLTYDSDDEVTQF
                     LCFVTAGVRLTDAKTGKVLWEDNQLVGETTFYTRAAGQSSDRLRGNAEFFLPTVRSFA
                     SEEENRGAAEALEQLASDIFLRTIEPW"
     gene            2968..>3738
                     /gene="holA"
                     /locus_tag="PKW60_11545"
     CDS             2968..>3738
                     /gene="holA"
                     /locus_tag="PKW60_11545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPK00112.1"
                     /translation="MDIQEFARSIETTPPGPVTLFCPHKGPRAREATYEPLLVERAIE
                     RIVTLYIEPGMRDMAYNAYFADEADPAEIVSVAETLPFLSERRIVVVRRAERYTSEAA
                     AGPILAYLGNPCPSTLLLLVADSMDRRSKFYKACDAAGAVVPCPQLNDAEAARWVHAE
                     VGTRAKKIHGEAVKRLIERTGLRLGDVQNAIHLVCDYVGGKESIDVEDVEAACADVAE
                     ERVWTLTDAIAASDVGAALRTLRELLDMGVSEFQILGSL"
BASE COUNT          803 a         1054 c         1144 g          737 t
ORIGIN      
        1 gcacgccaac gagtagacag cccccttcac aggataaaaa atacgtttgt gagaaccagt
       61 ttttgtagct gtgttcgtcg aggatctctt tgagtcgttc ctgggccaaa gcccagggat
      121 tggtttcctt cggcgccatt acccctcctg catcacaaac agccattccc tcacccccct
      181 cgcttacccg cgacgggatc ccgttgcgcg ctcgtcgatg atcggactgc ggcatcgtac
      241 ggcgtttacc ggcgttcccc tcgtgtccct tccgcgcgct tcaattcgcc ccaaattcag
      301 agtacaggga cctcaaaatt cacgaccagc ccaagtcgtt accggacaga aaaagccgaa
      361 ccatggcacg ggccacggca cggctccggc tcgatatccc tgttatccac aagttatcca
      421 caatcgctcc acaaccctgt aaataaaggc cgaaacgcga tcgcatgagt ctgcgagtgt
      481 gcaacaaatc gcatcaaaag tcaagcgtac tattcagtgt ggcgctcggc attcttaacc
      541 cgttgccata cttgatccat ctcttcgagg gaacagtcct caatcctccg cccggcctgt
      601 tcgagcgcgt ctcttacctt tccaaaacgc gcgagaaagc ggtgattggt ggcctccagc
      661 tcacgggatg ggtcggcatt cacgaagcgg gcaaggttca ccaccgcaaa aagcaggtca
      721 cccagttcac ggcggacgtc atcaggctgg ccgtgggcca gggcgtcgcg aatctcgtca
      781 agctcctcca ggatcttgtc aaagacgggg gcaatgtctg gccaatcgaa cccaacgcgc
      841 gcggcatcaa gctggatctc aactgcattc tggagcgggt cgttcaggag tcgagactgc
      901 gcggtcactg gggttcctca tcttcttttg cgcgaaatac aggcgcggtt tcagctccag
      961 tcgggtttct gcgaaagggc cggcgagggc gcgctgcttc gtctgaactc cgtacggatt
     1021 gccgctgttg cgcgttttcc gaccgtaaat tcactataac atgaggtcac cgcgcagaca
     1081 agctgacttc ccgcgcagcg gtgaagaatg gagaaaatcc gcatgatgag tacacgcatg
     1141 cgttgacgtg tcgcgctgga atgggcggcc acagggggcc gcccctgcaa aatccggacg
     1201 gtggatgcgc gtaccgcgtg tccacctccg ccagcggggg gcataagacg cgaagtcggc
     1261 cgtgccggaa aacctggttg agttcggccc gggcgattgg tagtattttc cacaggctgg
     1321 tatgatggat agaatgcagc gtgccatgcg gcgcgccgga aagtgctggt ttcggtaagg
     1381 tggttccatc tcgaaaggct cgaacggctt cggaggacat aaggtgagcg tggtatcagt
     1441 ccggaaatgc ggtgtgcttc tggccctggg ccttcttgta gtggggacaa ccgcggacgc
     1501 gtgggtgtgg aatccggcca ccggacgctg gatcaacgag aagaacctcc cgcagcccac
     1561 tgcggagctt caggttgagg cagcccgttc gctgatggaa caaggcgaat acaaacgcgc
     1621 catgcgcgag acccacaaat ttgataagta ctatcttgat tcggaatggg ccgatgacaa
     1681 ccagttcctc cgaggcgaga taaatctccg gcggggcgac tatctgaaag cggccaacga
     1741 gttccagcag gtgatcacca attatccgga cacggatctg tatgatacgg tgattgcaaa
     1801 acagtatgag attggcgacc ggttctatgc gcagggcgag aaacggctgg ggaaacgatg
     1861 gtcgccgttt cggaaacgtc cgttcaagaa ggcgatcgaa gtgtactcga tggtgatcga
     1921 caaccagccc ttccaggaaa cggctgcgga ggcgcaatac aaggtcgggc tgtgccattt
     1981 cacacggaat gaatacgtgg aagctgccta cgaataccag cgtgtgctcg aagactacgc
     2041 cagttccgcc tgggtggacg acgcgagtta tggcctggcg atgtgttatt acaaggcctc
     2101 gctgcccccg gactacgatc agacgaggag caggctcgca attgatgcca tagacgagtt
     2161 caaggcgcgt ttccccggcg atgaacgcgc cgaaggactg gatgccaagc gcgcggagat
     2221 gagcgagaag atcgcacggc agcgactgga cacggcccgc ttctacgaga agcgccgccg
     2281 ttttaccgca gcccgcatgt attatgaagt cgtgctcgag caatttcctg agacctcagc
     2341 ggcggcggac gccaagctgt ggctcgagca gtccgtgcag caggagcaga cagcgcagcc
     2401 atgaaaaacc ggtgggccct ggcggggtgc gttgtgataa tggcgtgggc ggcggggtgt
     2461 ggctattcaa ctcacagcac gctcgatgca cagtaccaga cgatcgccgt gcccgccttc
     2521 agaaatctca gtagagaata tgacctgcag gccccgctca cgaatgcggt gatccggaaa
     2581 ttcatggccg attcccgcct ggaaatcgtg cccctggatc aggccgacct gattctggag
     2641 ggcgtcatcc tgggatacga gcgcaaagga ctgacctacg acagcgacga tgaagtgacc
     2701 cagtttctgt gttttgttac agccggcgtc cgtctgaccg atgcgaaaac gggtaaagtt
     2761 ctgtgggaag ataaccagtt ggtgggggag acgacgttct acacgcgcgc ggcggggcaa
     2821 tcctcagatc gattgcgggg gaacgcggag ttttttctcc caaccgtccg ctcgtttgct
     2881 tccgaagaag aaaatcgcgg ggcagcggaa gcactggaac agttggcttc ggacatcttc
     2941 ctgcgcacca tcgagccgtg gtgaattgtg gatatccagg aattcgcccg aagcattgag
     3001 actacccctc cgggtcctgt gacgcttttt tgcccgcaca aggggccgcg ggcccgggaa
     3061 gctacgtatg aacctctgct ggtggaacgc gccattgaac gcatcgtgac gctgtatatc
     3121 gaacccggca tgcgggatat ggcgtacaac gcgtattttg ccgacgaagc ggatccggct
     3181 gagattgtct ccgtggcaga gacgcttccc tttctctccg agcgccgtat tgtcgtggtg
     3241 cgccgtgcgg aacgatacac gtccgaagcg gcggctggcc cgatcctggc gtatctcgga
     3301 aatccatgcc cctccacgtt gctgctgctg gtggcggaca gcatggaccg ccggtcgaaa
     3361 ttctataagg cgtgcgacgc cgcaggggcc gtggtgccct gcccccaact caacgacgcc
     3421 gaggcggctc gctgggtcca tgcggaggtc ggcacacggg caaaaaagat ccatggcgag
     3481 gcagtcaaac gcctgatcga acggactggc ctgcggctgg gcgatgtgca aaacgccatc
     3541 catcttgtat gcgattatgt cggtggaaaa gagagcatcg acgtcgaaga cgttgaagcg
     3601 gcgtgtgccg atgtggcgga ggagcgcgtc tggacgctga ccgatgcgat cgctgcctcg
     3661 gatgtaggcg ccgcgctgcg caccctgcgg gaattgctcg atatgggcgt gagcgaattt
     3721 cagatcctgg gctcgtta
//
LOCUS       DAOPBQ010000382         3719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_20069_length_3719_cov_3.331605, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000382 DAOPBQ010000000
VERSION     DAOPBQ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20069_length_3719_cov_3.331605"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PKW60_11550"
     CDS             <1..560
                     /locus_tag="PKW60_11550"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559212.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPK00113.1"
                     /translation="KELAHQEAIIRRVADEVQKAHKITLKYMVGTMIEIPRAALTADM
                     IAGVAEFFSFGTNDLTQMGFGYSRDDVGGFLPLYLDQKILAEDPFQVLDQEGIGQLVE
                     IGIQKGRSTRPDLKVGICGEHGGEPKSVEFCHRVGMNYVSCSPFRVPIARFAAAQAAL
                     EWPRKPAKKTAAKKSAAKKKARAKK"
     gene            complement(835..1596)
                     /locus_tag="PKW60_11555"
     CDS             complement(835..1596)
                     /locus_tag="PKW60_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00114.1"
                     /translation="MHCLIAAILVAGLSQGPATQEIVCPGTYGGHLQGLATDNEQNIY
                     WSFTVALVKTNRSGELLKTVSAPSHQGDLTWVDGKVYVAVNLGKFNQEPGQADSWVYI
                     YDDQDLTLLEKKAVPELVHGAGGMAFHEGKFIVVGGLPVGYSENYAYEYDRDFNFVKR
                     HVIQSGYTKMGIQTTCWFDGAWWFGCYEDDKGLLKTDEHFTLLGRFNPNYSVGIAPWT
                     DGNCLRGVNHPQENKQHSASAVVDAPGKTTETAEK"
     gene            complement(1800..2606)
                     /locus_tag="PKW60_11560"
     CDS             complement(1800..2606)
                     /locus_tag="PKW60_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPK00115.1"
                     /translation="MRKVFSVLMLSILAMAGCMTLEGEPVKFNILSTQQEIQLGKQLS
                     AEVEKQEKVSKDAALQAYVSQIGARLGSVATRQDVQYSFTVIDNPDTVNAFALPGGHM
                     YLYTGLLKICENEAELAGVMAHEIAHVAAHHHGEAMTKQFGFELLTQLVLGEQPSALA
                     KIATGVFGQGMMSYYSRDNEREADALGMEFLYRAGYRPDGMVSFMQKLIAEEQRSGGG
                     HPLPIFASHPPTQERLARLQNQLQRYPQAELQQISLNQDRYRENVLKRLK"
     gene            complement(2655..>3719)
                     /gene="hisC"
                     /locus_tag="PKW60_11565"
     CDS             complement(2655..>3719)
                     /gene="hisC"
                     /locus_tag="PKW60_11565"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPK00116.1"
                     /translation="RGDTEMKYARALLVDVEGYVPGEQPKDPDVVKLNTNENPYPPSP
                     RALEALTQVSADALRKYPDPLSIQLRARWAEIQGYPGPEWVIAGNGMDELLAMAVRTF
                     VDPGDSILTTSPTYTLYDTLARLHGARVEAVALDAEYQLPVSFFGHGGRLCFMPRPNA
                     PTGTCAPREDVERFCSGFQGLVVLDEAYVDFADDNCMDFAKRHDNVIVMRTFSKSFSL
                     AGLRLGLGVAHPAIINEFLKTKDSYNLNAVTQKVGLAALHDYEYMTRNAARVAATRKR
                     ATAALEALGFTVTPSQANFVLAHWSGKPEAKAIFERLKDRRIFVRYFDAPRLRDALRI
                     SIGTDPEMDALVAALRDILQ"
BASE COUNT          706 a         1113 c         1111 g          789 t
ORIGIN      
        1 tcaaagagct ggcccatcag gaagccatca tccgtcgtgt agcggatgag gtgcagaagg
       61 cccacaagat cacgctgaag tacatggtcg gcacgatgat cgaaattccc cgcgccgcgc
      121 tcacggccga catgattgcc ggggtggccg agttcttcag cttcggcacc aacgacctga
      181 cccagatggg gttcgggtat tcacgcgacg acgtcggcgg tttcctgccc ctttacctcg
      241 accagaagat tttggccgaa gacccgttcc aggtcctgga ccaggaaggc atcggtcagt
      301 tggtcgaaat cggcatccag aagggccgct ccacgcgtcc ggacctcaag gtcgggattt
      361 gcggcgagca cggcggcgag ccgaagtcgg tggagttctg ccaccgtgtg ggtatgaact
      421 acgtgagctg cagcccgttc cgcgtgccga tcgcgcgttt cgcggcagct caggccgccc
      481 tcgaatggcc gcgcaagccc gccaagaaaa cggctgccaa gaaatccgca gcgaagaaga
      541 aggctcgcgc gaagaagtaa aaacggcttc ttggtgaaac agacgattgg aaccagcccc
      601 cgcgaaggca atggcctcgc gggggctttt ctgtgtcgct gcgctcgtga ccacagtggc
      661 tgttgccggg ggaagcgccc agcctggccg gttacagcat ctgagttcgc gaccggtggg
      721 cgtcgtgtcc ccgcgccgcc ggtatagggt gcgatgggag attggcaggg gcggcgcctg
      781 cgtcgcccct gtatcaacgc gcaggatttc agccgccttc acggaagaga ggcgctattt
      841 ctccgcagtc tccgttgttt tgcccggtgc atcgacgacg gcggaggcgg agtgctgctt
      901 attttcctgg ggatgattta cgccgcggag gcagttgccg tctgtccagg gggcgatgcc
      961 cacggagtaa ttggggttga agcggcccaa cagcgtgaag tgctcgtcgg ttttcaacag
     1021 gcccttgtca tcctcatagc acccgaacca ccacgcgcca tcgaaccagc aggtggtctg
     1081 gatccccatt tttgtgtagc cgctctgaat cacatgccgt ttcacgaaat tgaagtcccg
     1141 gtcgtattca taggcgtaat tctcagagta gcccaccgga agtcctccca cgacgatgaa
     1201 ttttccttcg tgaaaggcca tccctccggc gccatgcacc agttccggca cggccttctt
     1261 ttccagcagt gtgagatcct ggtcgtcata gatgtacacc caggaatccg cctggccggg
     1321 ctcctggttg aatttgccga ggttcaccgc gacgtatact ttgccgtcaa cccaggtcag
     1381 gtcgccctga tggctcgggg cggatacggt tttcagcagc tcgcccgagc gattggtctt
     1441 gaccagggcc acggtgaacg accaataaat gttctgttcg ttatccgtag cgagcccctg
     1501 caagtgtcct ccgtaggtac ccggacacac gatttcctgg gttgcaggac cctgactcaa
     1561 cccggcgaca agaatggcgg caatcagaca atgcatcaga caagccctcc tatgctgttt
     1621 tcagtgactt tacggatttc aggcagcgag attcaacacg ggggctcttt ttgccggcaa
     1681 aagaggcctt cggggaagaa atgtggcatg cgggcgtgct ttcgctggaa aatgtcaggt
     1741 tttgcgggcg gactgcagcc gctggagaga gagtcgccgc ccacctgcat ccgcgctcgt
     1801 tatttgagcc gttttaatac attctcgcgg tatcggtcct ggttcaggga gatctgttgc
     1861 agttcggcct gcggatatct ctgcagctgg ttctgcagcc gggccaggcg ttcctgggtg
     1921 gggggatgcg atgcaaagat gggaagcggg tggcctccgc cgctgcgttg ctcttccgcg
     1981 atcagtttct gcataaacga caccatgcca tcgggccggt atcccgcgcg gtacaggaac
     2041 tccatcccca gcgcgtcggc ttcccgttcg ttgtcgcggc tgtaataact catcatcccc
     2101 tgtccgaaga cccccgttgc gattttcgca agcgcactgg gctgttcgcc gagcaccagc
     2161 tgagtcaaca gttcaaagcc gaactgcttg gtcatggctt ctccgtgatg gtgcgccgcg
     2221 acgtgcgcga tctcgtgggc catcacaccg gcgagctctg cctcgttttc acagattttc
     2281 agcagccccg tatagaggta catatgcccg ccgggcaggg cgaacgcgtt gacggtgtcc
     2341 gggttatcga tcaccgtaaa actgtactgg acgtcctggc gtgtggcgac gctgccgagc
     2401 cgcgcgccaa tttgcgagac gtaggcctgc aacgcggcgt ctttggaaac cttttcctgc
     2461 ttctcgactt ctgctgaaag ctgtttgccc agctgaattt cctgttgcgt ggagagaata
     2521 ttgaatttga cgggctcgcc ttccagggtc atgcagcccg ccatcgcgag aatgctcagc
     2581 atgagcaccg aaaacacctt gcgcattgcc gttcctttcc ataacttgac tgtcattgcg
     2641 caatccatcg tacgctattg cagtatatcc cgcaacgccg caacgagcgc atccatttcc
     2701 gggtccgtgc cgatgctgat gcgcagcgca tcccgaagcc ggggggcgtc aaaatagcgt
     2761 acaaatatcc ggcgatcttt caggcgttca aatatggcct tagcctctgg tttgccgctc
     2821 cagtgggcca gcacaaagtt cgcctgcgac ggcgtcactg tgaagccgag cgcttccagg
     2881 gcagccgtgg cgcgttttct ggttgccgcc acccgggctg cgttacgcgt catatactca
     2941 taatcgtgta acgctgccag gccgactttc tgcgtaaccg cattgaggtt gtacgagtct
     3001 ttcgtcttca gaaattcgtt gatgatagcg gggtgcgcta ctcccagccc cagccgtaac
     3061 ccggccaggc tgaacgattt tgaaaaggtc cgcatcacga taacgttgtc gtggcgttta
     3121 gcgaaatcca tgcagttgtc gtcggcgaag tcgacgtagg cctcatcaag cacgaccagc
     3181 ccctgaaaac cgctgcagaa gcgctcaacg tcctcgcgcg gcgcgcaggt gcccgtggga
     3241 gcattcggcc gcggcataaa acagaggcgc ccgccgtgcc cgaagaacga caccgggagc
     3301 tggtactcgg catccagcgc caccgcctcc acccgcgcgc catgcagccg cgccagcgtg
     3361 tcatagaggg tgtaggtcgg ggaggtcgtc aggatgctgt cgccgggatc cacaaacgtg
     3421 cggacggcca tggccaggag ctcatccatg ccgttgccgg caatcaccca ttcgggaccg
     3481 gggtagccct gtatctccgc ccaccgggcc cgcagctgaa tcgacagcgg gtcggggtat
     3541 ttgcgcaacg catccgcgct cacctgcgtg agtgcttcca gcgccctggg agatggggga
     3601 taggggtttt cgttggtatt gagtttgaca acatccggat ctttcggctg ttcaccgggg
     3661 acgtagcctt ccacgtcaac cagcaatgcg cgtgcatact tcatttccgt atctccacg
//
LOCUS       DAOPBQ010000383         3692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_20255_length_3692_cov_3.712950, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000383 DAOPBQ010000000
VERSION     DAOPBQ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20255_length_3692_cov_3.712950"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PKW60_11570"
     CDS             complement(<1..432)
                     /locus_tag="PKW60_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00117.1"
                     /translation="MSAVDAAPDNRNRAMHARRTWIRLMEIAGALVVALLVYNGLAFN
                     LALHGEDHKSRNPETGLLAGAEPRDLGPEDSPCAALFIHGFGGAGTDFAGLPERLAAT
                     GWRVRVMLLPGHGTHPRDLSRQAPDTLLTAVHDEVLRLRERH"
     gene            complement(419..>3692)
                     /locus_tag="PKW60_11575"
     CDS             complement(419..>3692)
                     /locus_tag="PKW60_11575"
                     /inference="COORDINATES: protein motif:HMM:NF006386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HPK00118.1"
                     /translation="VLAALGLLMFALPYLLFPGLTGALADRYSKRKVTIATKWWEVGV
                     MVVGLLAFWIDSVPLMAVMFFMMNMQSAFFSPAKYGILPEMLPESRLSWGNGILQMAT
                     FVAIILGVGIVGPLMDLGLGVYWLSTLLILLSTLGLVASYQVTHVAAACPDRRISFNP
                     WSGLGKYFQCFRRDHVLFLTMVGGGFFWFFGGLMQSNVIVLGESVLNWSHQYVGYLQL
                     AVALGIGLGSVTAGYLSRRKIELGLVPLGLAGMTVMAGLLYLPWTSFPVLTALLFALG
                     WSAGFFEVPLAASLQHRSPREMKGGIIAAYNIVTCLGISIAGLVLLIQPIFVPTGQKV
                     NPYNIFIAGAGMTLLMGLYLCLRLPMFILRTVLWILGNTIYRVDTHGRHHIPEKTGAL
                     IVANHTSFVDALILTASIDRPVRFLISQEIYAVRWIRPLAKAMRAIPVSPMAQPKDLI
                     RALHNATDAINNGELVCIFAEGQITRTGNLLPFRRGFERIMKGTDAPIIPAHLDRLWG
                     SIFSYSEGRFFLKLPKRIPYRVVVTFGAPMNATSPTVEVRERIQRLGTEAYLDRKLHE
                     PLLHRGFIRMARKRPFKMAMADQSTEGIPYWKALVGSIALARKLRTVLDGQPMVGVLL
                     PPTVGAALTNIALTLMGKTVVNLNYTASDDALESAARRCNLTQVLTAHAFLNKVSCAV
                     PAKAVYLEDIRKTIGGFDQLMAIFMAALLPMRLLERRLGGPARRSPDDLATIIFSSGS
                     EGEPKGVMLSHFNIMSNIESALQVFPHHRGDGMMGILPFFHSFGYMATLWLPLAQGFR
                     AVFHPNPLEAKVIGQQIYKYKLDFLVAAPTFLQSFIRRCLPEELACLRYVITGAEKLP
                     VRVREAFESKFGLTPLEGYGTTECGPAVSFNVIDFRAPGFYQVGTKHGTVGHPIPGVS
                     VKIIDPDTGRELPTGEAGLLHVKGPSIMKGYIGMPDKTAEVLHNGWYNTGDVAFVDED
                     GFITITDRLARFSKIAGEMVPHTKVEEELHRLLGLSEQSMAVTGVPDSGKGERLVVLH
                     TLSNGDVERLVARMDQSSLPKLWLPKYNSFYYIPEIPILGTGKMDIKQVRKIAKELDV
                     GG"
BASE COUNT          748 a         1149 c         1125 g          670 t
ORIGIN      
        1 gtggcgttcc cggagcctga gcacctcatc atggacggca gtgagcaggg tgtcgggggc
       61 ctgcctgctc aaatcgcgcg ggtgggtgcc gtgacccggc agaagcatga cgcgcacgcg
      121 ccagccagtt gcggccagac gctcaggaag gccggcgaaa tccgtgcccg caccgccaaa
      181 tccatgaatg aacagcgccg cgcacgggct gtcttcgggg ccgaggtccc ggggctccgc
      241 accggccaga agccccgttt ccggattgcg cgatttgtga tcttcgccgt gaagcgccag
      301 gttgaatgcc aggccgttgt acactagcaa agcgacaacc agcgcacccg cgatttccat
      361 caggcgaatc cacgtccgcc gggcgtgcat cgcccggttg cggttatccg gcgcggcgtc
      421 aaccgccgac atccagctct ttggcaatct tccgtacctg tttgatgtcc atcttccccg
      481 tgccgagaat ggggatctct ggaatgtaat agaaggagtt gtatttgggc agccacaact
      541 tgggcaggct cgactggtcc atccgcgcca ccaggcgctc cacatcgccg ttgctgaggg
      601 tatgcaacac caccagccgc tcgcctttgc ctgagtcggg cacgccggtc acggccatgc
      661 tctgctcgct caggccaagc aggcgatgca gttcttcttc gaccttggtg tgcggcacca
      721 tctcgccggc gattttgctg aaccgtgcca gccggtccgt gatggtgatg aacccgtctt
      781 catcgacaaa agcgacgtcg ccggtgttgt accagccgtt gtggagcact tcggcggtct
      841 tgtcgggcat gccgatatag cccttcatga tgctggggcc tttgacatgc aggagcccgg
      901 cctctcccgt gggcaactcc cggcccgtgt cgggatcaat gattttcacg ctgacgcccg
      961 ggatcggatg gcccactgtg ccgtgcttgg tgccgacctg atagaacccc ggcgcgcgaa
     1021 aatcgatcac gttaaacgac acggccgggc cgcattccgt ggtgccgtag ccttcaagcg
     1081 gggtgaggcc gaacttgctt tcgaaggctt cgcgcacccg gaccggcagc ttctccgctc
     1141 cggtgattac atagcgcaaa caggcaagct cctccggcag gcaccgccgg atgaacgact
     1201 gcagaaacgt cggcgccgcc accagaaagt cgagcttgta tttgtaaatc tgctgcccga
     1261 tcacctttgc ttcgagcgga tttggatgga atactgcccg gaacccctga gcaagcggca
     1321 gccacagcgt cgccatgtag ccaaaggaat ggaaaaacgg caggatgccc atcatgccgt
     1381 ctccacgatg atgcgggaat acctgcagcg ccgattcgat attactcatg atgttgaaat
     1441 ggctcagcat gacccccttg ggctcgcctt cgcttccgct gctgaagatg atcgtcgcca
     1501 gatcgtccgg agaacggcgg gccggtccgc caagccggcg ctcgagcagg cgcatgggca
     1561 acagcgccgc catgaagatg gccatcagct ggtcaaaacc cccgatcgtc ttgcggatgt
     1621 cctcaagata gacggctttc gccggcacgg cgcagctcac tttgttcagg aatgcgtggg
     1681 ccgtgagcac ttgcgtcaga ttgcagcggc gcgccgcgct ttccagggcg tcgtccgatg
     1741 ccgtgtaatt gaggttcacg acggtctttc ccatgagcgt cagcgcaatg ttggtgaggg
     1801 ccgcgccgac cgtcggcggc agcagcacgc cgaccatggg ttgcccgtcg agcaccgtgc
     1861 gcagtttgcg cgcgagcgcg atgctgccca ccagcgcttt ccagtagggg atgccttcgg
     1921 tggactggtc cgccatggcc attttaaagg ggcgtttgcg cgccattcgg ataaatccgc
     1981 ggtgcaacag cggctcatga agcttgcggt ccaggtaggc ttccgtgccg agccgttgaa
     2041 ttcgctcgcg cacctccacc gtcgggctgg tcgcgttcat cggcgcgccg aaggtcacga
     2101 ccacgcggta ggggatgcgc ttcgggagtt tcaggaagaa gcgcccttcc gaatagctga
     2161 aaatgctccc ccacaagcgg tccaggtgcg cggggatgat cggcgcatcc gtgcctttca
     2221 tgatgcgctc aaatcctctg cggaacggca agaggttgcc cgtgcgcgtg atttgccctt
     2281 ccgcgaatat gcatacgagt tctccgttgt tgatcgcatc cgtggcgttg tgcagggcgc
     2341 ggattaaatc tttgggctga gccatcggcg acaccgggat cgcgcgcatg gcctttgcaa
     2401 gggggcgaat ccaccgcacg gcgtagattt cctgtgaaat cagaaagcgt accggacgat
     2461 cgatcgaggc cgtgaggatc agcgcatcca cgaacgaggt gtgattggcg acgatgagcg
     2521 cacccgtctt ttccgggatg tgatgccggc cgtgggtgtc gacgcggtat atcgtgttac
     2581 ccaggatcca aagcaccgtc cgcaggatga acatggggag gcgcaggcaa aggtagaggc
     2641 ccatcagcaa agtcatgccc gcgcccgcaa tgaagatgtt gtaggggttc accttctggc
     2701 ccgtgggcac gaagatcggt tgaatcagca gcacgaggcc ggcgatcgag atgcccaggc
     2761 aggtcacgat gttgtacgcc gcgatgatgc cgcctttcat ctcgcgcggg ctgcgatgct
     2821 gcagtgatgc ggccagcgga acttcaaaaa aaccggccga ccagcccagc gcaaagagga
     2881 gcgccgtcag cacaggaaag gaggtccagg gcaaatacag caacccggcc ataaccgtca
     2941 tgcctgccag tcccagcggc accaggccca gctcgatttt ccggcgcgac agataacccg
     3001 ctgtcacgct tcccaggccg atgcccaggg cgaccgcaag ttgcagataa cccacgtact
     3061 ggtgcgacca gttcagaacg ctttcgccca gcacgatgac gttgctctgc atcagaccgc
     3121 cgaaaaacca gaagaacccg ccgccgacca tcgtcaggaa cagcacatga tcccgccgga
     3181 agcattgaaa atacttcccc agacccgacc agggattaaa ggaaatgcgc cggtccgggc
     3241 atgccgccgc cacgtgtgtc acctgatacg aggcgaccag gcccagggtc gacaagagga
     3301 tgagcagcgt ggacagccag tagacgccca ggcccagatc catcaggggg cccacgatgc
     3361 ccacgcccag gatgatcgcg acaaacgtcg ccatttgaag gatgccattg ccccacgaca
     3421 gccggctttc cggcagcatc tccggcagaa tcccgtattt ggcggggctg aaaaatgcgc
     3481 tctgcatgtt catcatgaag aacatcaccg ccatcaaggg gacgctgtca atccagaagg
     3541 ccagcaatcc gacaaccatc acgcccactt cccaccattt ggtcgcgata gtcaccttcc
     3601 gtttactgta ccggtcggcc agcgcgccgg tcagccccgg gaacagaagg tacggaaggg
     3661 caaacatcaa caagccgagg gctgcaagca cg
//
LOCUS       DAOPBQ010000384         3689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_20281_length_3689_cov_3.681618, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000384 DAOPBQ010000000
VERSION     DAOPBQ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20281_length_3689_cov_3.681618"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..246)
                     /locus_tag="PKW60_11580"
     CDS             complement(4..246)
                     /locus_tag="PKW60_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK00119.1"
                     /translation="MNALRILIVEDDDVQREIICDILREAGYTVEAFGSPRKAVAALQ
                     KSTCDLLLTDLRMPEMDGLDLLREALRGLRAKAGPE"
     gene            complement(243..1595)
                     /locus_tag="PKW60_11585"
     CDS             complement(243..1595)
                     /locus_tag="PKW60_11585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK00120.1"
                     /translation="MPTTYPRIRWRQSLIVRVIILCALLVVCLLGAVYVATRFYFARV
                     VEEMHEQTGKIANIVSLYYEENPAAPLEDLKAELSREGADIQFLDGEAMQTLVTIEHD
                     EQGQMYKVAYSTLPLPDGRQALLTVRLNLQPQTEIIKAFRNKYLLTLTLGFLLTIGLM
                     VYFIAKTLRPLRELTQRCSRISQGHLENVEIRKNAGEILALEHTFNGMVNALKEKELM
                     EANLRQAQRLSALGSLAAGVAHDIRNPLNSIKLLSSHVSDTLDKDGDRERAVKQVRTI
                     RQEVDRLEEIVTGFLSMAREQELNPAPSKVDALLDECVQLIHKDAEQRGVRLTHELRA
                     GETELMLDSKQWKRAVINVLLNALEATPPGGRVRVFSRVTDESCEVEVRDDGPGMPPE
                     VTERAFDPYFTTKETGTGLGLSITRGIVEEHGGTIHLTTREQAGCQVLITLPLRNLQL
                     "
     gene            complement(1612..2553)
                     /locus_tag="PKW60_11590"
     CDS             complement(1612..2553)
                     /locus_tag="PKW60_11590"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPK00121.1"
                     /translation="MDHKTQDNAAAAFAELVREASLLVEQTAHVRKRTLSLSSEVAAL
                     FAQMGPADSSAAPAGAVNSPDGPTDAVQASVAGAATEPAGAVQAGAVDSPAGPADAGR
                     ERLADWAALQAEVAECRNCGLADSRTKTVFGAGSLDAELVFVGEAPGEHEDLQGLPFV
                     GRAGAFLTDVIEKGLKLTRDDVFICNVLKCRPPGNRDPKPDEREACEPYLIRQLALIR
                     PKVICALGGHAAKMLLKTEAPVGQLRGKWHFYQGIPVRVTYHPSYIVRSENEPARHKA
                     DKHKVWEDIKEVIRVLNGEIAPRPASGEPGGGPAYSG"
     gene            complement(2825..2900)
                     /locus_tag="PKW60_11595"
     tRNA            complement(2825..2900)
                     /locus_tag="PKW60_11595"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2865..2867),aa:Lys,seq:ttt)
     gene            complement(3110..>3689)
                     /locus_tag="PKW60_11600"
     CDS             complement(3110..>3689)
                     /locus_tag="PKW60_11600"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPK00122.1"
                     /translation="IGLGLRIATVGCTPPHTSGRNTKKQVAEELNTTGMYSVVRNPLY
                     LGNFLVGLGPALFLRIWWIPVIFLLAFFLYYERIIFAEEMFLRRKFEDLYIHWANATP
                     AFLPRWRQWRPSKYPFSLKKILRREHQTLFGLILIFYLIETITEIVMGNSPRLALDWP
                     DPVWNIILALGLAVFILLRLLRKRTTLLQENR"
BASE COUNT          716 a         1199 c         1058 g          716 t
ORIGIN      
        1 cccctactcg gggccggcct tggcgcgcag gccgcgcagc gcttcgcgca acaggtcgag
       61 cccatccatc tcgggcatgc gcagatcggt caacagcagg tcgcaggtgc ttttctggag
      121 cgcggcaacc gccttgcgcg gcgaaccgaa ggcctccacc gtgtacccgg cctcgcgcag
      181 gatatcgcag atgatctcac gctgcacgtc gtcgtcttcg actatcagga tccgaagcgc
      241 gttcatagct gcagattccg taagggcaaa gtgatcagca cctggcaccc ggcctgttcc
      301 cgggtcgtca ggtggatggt gcccccgtgt tcctcgacaa tgccccgggt gatggacaat
      361 cccaggcccg tgccggtctc tttggtcgta aaatacggat cgaacgcccg ttccgtcacc
      421 tcgggcggca tgcccgggcc gtcgtcgcgc acttcgacct cgcacgattc atccgttacc
      481 cgcgaaaaga cccgcacacg cccgcccggc ggcgttgcct ccagcgcatt caaaagcacg
      541 ttgatgaccg cccgcttcca ttgtttcgaa tcgagcatca gctcggtttc tcccgcgcgc
      601 agctcgtgcg tgaggcgaac tccccgctgc tcagcgtcct tatgaatcag ctggacgcac
      661 tcatcgagca gcgcatccac cttgctgggc gcgggattca gctcttgctc ccgggccatc
      721 gagagaaacc ccgtcacgat ctcctccagc cggtccactt cctggcggat cgtccgcacc
      781 tgtttcaccg cgcgttcgcg gtctccgtcc ttgtcgagcg tatcactgac atggctcgac
      841 agcagcttga tcgaattgag cggattgcgg atatcgtgcg cgacgcccgc cgcgagcgaa
      901 cccagcgccg aaagccgctg ggcctgccgc agattagctt ccatcagttc tttctctttc
      961 aacgcgttca ccataccgtt gaacgtatgc tccagcgcga ggatctcgcc cgcgtttttg
     1021 cggatctcca cattttcgag atgcccctgt gagatccggc tgcaccgctg cgtaagctcg
     1081 cgcagaggac gcagcgtttt ggcaatgaaa tataccatca gcccgatggt cagcaaaaag
     1141 cccagcgtca gcgtgagcag gtacttgttc cgaaacgcct tgatgatctc cgtctgcggc
     1201 tgcaggttca ggcgcacggt cagcagcgcc tggcggccgt caggcagcgg cagcgtggaa
     1261 taggccacct tgtacatctg cccctgctca tcgtgctcga tggtcaccag ggtctgcatc
     1321 gcttcgccgt cgagaaactg gatgtccgcc ccttcccgcg acagctccgc cttcagatct
     1381 tccagcggcg ccgccgggtt ttcctcgtaa tacagcgaga cgatgttcgc aatcttcccg
     1441 gtctgctcgt gcatctcctc gaccacccgc gcgaaataga agcgggtcgc cacatacacc
     1501 gcgccgagca ggcaaaccac cagcaatgcg cacaggatga tcacgcggac aatgagcgac
     1561 tggcgccagc gaatccgggg atatgtcgtc ggcatcccgt ccatgctgag gtcagccgga
     1621 atacgccgga ccgccacccg gttcgccgga cgccggccgc ggcgcgatct cgccgttcaa
     1681 gacccggatg acctccttga tgtcctccca gaccttgtgc ttgtcggcct tgtggcgggc
     1741 aggctcgttt tccgaacgca caatatacga aggatgatag gtcacccgca ccggtatgcc
     1801 ctgataaaaa tgccatttgc cgcgaagctg ccccaccgga gcctcggtct tcagcagcat
     1861 cttcgccgca tggccgccga gcgcgcagat caccttgggc cggatcagcg cgagctgccg
     1921 gatcagatac ggttcgcagg cctcccgttc atccggcttc ggatcgcggt tccccggcgg
     1981 acggcatttc agcacattgc agataaacac gtcatcgcgg gtcagtttca gccctttttc
     2041 gatgacatcg gtgagaaacg cgcccgcccg tcccacaaac ggaagaccct gcaaatcctc
     2101 atgctcgccc ggggcttccc ccacaaacac cagctcggca tcgaggctgc ccgcgccaaa
     2161 cacggtcttt gtgcgcgaat ccgccaagcc gcagttccgg cattccgcca cctccgcctg
     2221 cagcgccgcc caatccgcca gacgctcccg ccccgcgtcc gccggcccgg caggggaatc
     2281 caccgcaccc gcctgcaccg cacccgccgg ttcagttgct gcgcccgcca cacttgcctg
     2341 caccgcatcc gtcggcccgt caggggagtt cactgcaccc gccggtgcgg cagaggagtc
     2401 cgccggaccc atttgcgcaa acaaggccgc aacctccgac gaaagcgaca aggtccgttt
     2461 ccgtacatgc gccgtctgct ctaccagcaa actcgcctca cgcaccagct ccgcaaacgc
     2521 cgccgccgca ttatcctgtg tcttgtgatc catagcccct atgataccat cccccggctt
     2581 gcggagacac aaaacgcact ggccgcctcg cttcggccct gctggctgag gtgtcgggcg
     2641 gatacagggg cgtttcatca acgcagccgc gcatttttgc gggttttgtg tcgcggcgcc
     2701 tgggaaaggt gtcagcgagt gtgaaatgtc gcgaaaaatg gtttggtggt gcgccctgtt
     2761 tgtgccaggg tcggcaggat gcaaaaaagg actcacgcaa ctaagcgtaa gtccttatat
     2821 tcattggtga acggggcggg actcgaaccc gcggccaccg gattaaaagt ccgatgctct
     2881 accacctgag ctacccgtcc acggaattcg cggcaggaaa caaaaccggg ctggtacagc
     2941 ccggtaatac gatggatttt accggatgac gggtctgaag tcaaacggtt ccggtaaggt
     3001 ttagccaacg cccattcata ttctgagtgc acgagttcag tttttcaggg tttggacgtc
     3061 ctttttcctg ttggcgggtc tggggcgggg ctcgatgaag ggtcggccgt taacggtttt
     3121 cctgcagtaa cgtggtacgt ttccgcagga gccgcagaag aatgaacacg gccaggccca
     3181 gcgcgaggat gatattccac acagggtcgg gccaatcgag cgcaagacgc ggcgaattcc
     3241 ccatgacgat ctcggtgatg gtctcaatca ggtaaaagat caggatgagt ccaaacaagg
     3301 tctgatgttc acggcgaagg atctttttca gcgagaacgg atatttggaa gggcgccatt
     3361 ggcgccatcg aggcagaaag gccggggtcg cattggccca gtggatgtag agatcctcga
     3421 atttccggcg cagaaacatc tcctccgcaa agatgatgcg ttcgtagtag aggaagaaag
     3481 ccagcaggaa gatgacgggg atccaccaga tccgcagaaa gagtgccggg cccagtccca
     3541 ccagaaaatt gcccagatac agggggttcc gcaccaccga gtacatcccg gtcgtattga
     3601 gttcttccgc gacttgtttt ttggtgttgc ggcccgaggt gtgcggcggt gtgcatccaa
     3661 cggttgcaat gcgaagccca aggccgatg
//
LOCUS       DAOPBQ010000385         3688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_20290_length_3688_cov_4.108175, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000385 DAOPBQ010000000
VERSION     DAOPBQ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20290_length_3688_cov_4.108175"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PKW60_11605"
     CDS             <1..571
                     /locus_tag="PKW60_11605"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPK00123.1"
                     /translation="RLQAEMREAVANARGNSASLTLEFRRRLKDGSYRWVSDSLMLVL
                     DDHGSVEAIVGSIHSIEERKRAEEALRREGQHLQKLLQAAPVILFAADRDGVCTAIEG
                     RELEVLAIDPAQFVGKPIREICADRPGMKTLLDRALTGEEFSAEYLDERTGRRLVGHV
                     AQLRDEQGQREGIVVICVDTAEQKQPGMP"
     gene            complement(617..1123)
                     /locus_tag="PKW60_11610"
     CDS             complement(617..1123)
                     /locus_tag="PKW60_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00124.1"
                     /translation="MLSILIRGIVGFIFVLLGVVGLVSPGTTRRLVSALLAKAPVRVF
                     GAFLMLLGAGAFRAADQLSLPLAGQVLGVALFMAGGVHILIPDFAIILNEWWVAQKTI
                     WERLVSVVYLFLAALFLAPSEGIHVRQWFTPLLPAAYDTPSEDEDDDDPEVTAPEPAP
                     HDTAPTPE"
     gene            complement(1250..2416)
                     /locus_tag="PKW60_11615"
     CDS             complement(1250..2416)
                     /locus_tag="PKW60_11615"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPK00125.1"
                     /translation="MERIAKSCLVLLALSGLVAAPVLAEGYLTTVGDGADTISVVVVR
                     GTPYEMGHAFGTLMRDEVAACMGGYLAYARQSGVPLYSNESLDAAWAAVAPHTDQRFK
                     DELRGLADASGVAMEELIRAHMIPVVSSYSCSGVAVWNEATANGNLYQIRNLDFTMNA
                     GLQDYPVVVVYLPQQGLAHANVTFAGCIGCNTGMNVAGIALSEKGASPRSDMPYDING
                     EHFMMLFRDILYDAGDLETAVRLVKESPRIKKYRFYIGDGQGKKAVKIKAYAPDLDIW
                     PDNDPTDEVAPNILKNVIYYTMNNQAAFTHLTENYGKYDAESVIALSRLVADDDGNLL
                     NVVYDATALEMRVAYAEGPATAATRPYVALRLRDYLNYNPEGAIKTADTPKQTN"
     gene            complement(2658..3473)
                     /locus_tag="PKW60_11620"
     CDS             complement(2658..3473)
                     /locus_tag="PKW60_11620"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HPK00126.1"
                     /translation="MGTSSDPRAQVTRTDIARGLDALGIRPGEIVYVHSSLSAFGHVH
                     GGADTVIDALLDTVGAQGTVVMPTFTWQRNHARPTLVFNVAHDPSETGRITEVFRRRR
                     GVVRNEHVCHSTAAFGPAADDVMGGSVHPFAFDASLYRCYELNSWYLLLGCGFSSATA
                     LHTAEEIVQVPYRHYRNFQGSVVIRPDGTHVPAESVEFLRNPGYANDFAKMDAVFERD
                     GILRHARVGNALLTSARIRDVIDRAVQYLKQDIGYLLDEPSRARWRAAQEREA"
BASE COUNT          718 a         1181 c         1108 g          681 t
ORIGIN      
        1 gcggcttcag gccgagatgc gggaggccgt tgcgaacgcg cggggcaatt ctgcgagcct
       61 cacgctcgaa tttcgccgca ggctcaaaga cggcagctac cgctgggtca gcgattcgct
      121 gatgcttgtg ctcgatgacc atggctctgt tgaagcgatt gtaggctcca tccacagcat
      181 tgaggagcgg aaacgggccg aagaagcctt gcggcgcgaa gggcagcatc tgcaaaaact
      241 gcttcaggcc gcgcccgtga tcctgttcgc cgccgaccgc gatggggtgt gcaccgccat
      301 cgaaggccgg gagctcgagg tgcttgccat cgacccggcg cagttcgtcg gcaaaccgat
      361 ccgggagatc tgcgcggacc gcccgggaat gaagacgctg ctcgatcgcg ccctgaccgg
      421 cgaagaattt tcagccgaat acctggacga gcgcactggg cgcagactgg tcggacatgt
      481 cgcacaactg cgtgacgagc agggccagcg cgagggcatc gtcgtcattt gcgtcgacac
      541 cgctgaacaa aaacagcccg gcatgccctg agcaacgccc gggcatgcag agcgcgtttc
      601 acgggccgct gtgggctcat tccggtgttg gcgcggtatc gtgtggggcg ggctccggcg
      661 cagtcacttc cgggtcgtca tcgtcttcgt cttcagatgg ggtgtcgtac gccgccggca
      721 gcagtggcgt gaaccactgc cgcacgtgaa tcccttctga cggggcgagg aacagggccg
      781 ccaggaagag gtagacgacg ctgaccaggc gttcccagat ggtcttctgg gccacccacc
      841 attcattcaa aattatggcg aaatcgggaa tcaggatgtg cacgccgccc gccatgaaga
      901 gcgccacgcc gagcacctgg ccggcaagcg gcagagagag ctggtccgcc gcgcgaaacg
      961 cgcccgcacc cagcagcatg agaaaagcgc caaagactcg aaccggggcc ttcgcgagaa
     1021 gcgcgctgac cagcctgcgc gttgtacccg gcgatacgag ccctaccacg ccaagaagca
     1081 caaagataaa ccctacgatg ccgcggataa gaatcgacaa catcgccgcc tccttcaact
     1141 tcctgccctg gcgggcaacg tcctcctgtc tcgtcgagtg cagaccctga tttactgacg
     1201 caccttccgc gtgtcaatcc tgtcgctcgc ggatggcgca ggcgggcaat cagtttgtct
     1261 gtttcggcgt gtctgccgtc ttgattgcgc cctcagggtt gtagttcaga taatcccgca
     1321 ggcgcagcgc gacatagggc cgtgtcgcgg cggtcgcggg cccttccgca taggcaaccc
     1381 gcatctcgag cgccgtggcg tcataaacga cgttgagcag gttgccgtca tcgtcggcta
     1441 caagccgtga cagcgcgatc accgattcgg catcgtattt tccgtaattt tcggtgaggt
     1501 gggtgaaggc tgcctgattg ttcatcgtgt aataaatgac atttttcagg atattcggcg
     1561 cgacttcgtc ggtggggtca ttatcgggcc agatgtccag gtctggcgca taggccttga
     1621 tcttgactgc cttttttccc tggccgtcgc cgatgtagaa ccggtatttc ttgatgcgcg
     1681 gcgattcctt caccagacgg acggcggtct cgaggtcgcc cgcgtcatag aggatgtccc
     1741 ggaacagcat catgaaatgt tcgccgttaa tgtcgtacgg catatcgctg cggggggaag
     1801 cgcccttttc cgacagcgcg atccccgcca cgttcatgcc tgtgttgcag ccgatgcagc
     1861 cggcgaacgt gacgttggcg tgcgcgaggc cctgctgggg cagatacacc accaccaccg
     1921 gataatcctg caggccggca ttcattgtga agtcgaggtt gcggatctgg tacaggttgc
     1981 cgttggccgt cgcctcattc cacacggcca ccccgctgca ggagtacgac gacaccaccg
     2041 ggatcatgtg cgcgcggatc aattcctcca tggccacccc tgacgcatcg gccaggccgc
     2101 gcaactcgtc cttaaaccgc tgatccgtgt gcggcgccac ggcagcccag gccgcatcaa
     2161 gcgattcgtt gctgtacaga ggcacgccac tctgccgcgc ataggccagg tatccgccca
     2221 tgcacgcggc cacttcgtcc cgcatcagcg ttccaaacgc gtggcccatt tcatacggag
     2281 tgccccggac cactaccacc gagatggtat ctgcgccgtc gcccaccgtc gtgagatatc
     2341 cttccgccaa cacaggcgcg gccacgaggc cgctcaacgc caaaagcaca aggcagcttt
     2401 ttgctatccg ctccatgaca aaaccctttc actgtgatga caggaccgta ttcctgaatc
     2461 gtcccggatt acacaccata ttgactgaaa cacggacttc tgtgcaagaa aacaaatcgc
     2521 gcccagacgc acccgtaccc aggagagcaa gcacctgctt tttacgatct tgccagggca
     2581 tagcacggag cacgctccgt gtacgcgtct tcagcagagg cagataagcg gaccgcccgc
     2641 cccctgccag gccgctctca tgcctcgcgc tcctgagccg cacgccagcg cgcgcgggac
     2701 ggctcgtcca gcaggtaacc gatgtcttgt ttgaggtact gaacggccct gtcaatgaca
     2761 tcgcgaatcc gggcgctggt caacagggcg tttcccacgc gggcgtgccg caaaattcca
     2821 tcacgttcaa aaacagcgtc cattttggca aaatcgttgg cataacccgg atttcgcaga
     2881 aattcgaccg attcggccgg cacgtgagtc ccgtccgggc ggatcaccac cgaaccctgg
     2941 aagttgcgat aatgccggta gggaacctgg acgatttcct cggccgtgtg cagcgcggtc
     3001 gccgacgaaa atccgcaacc cagcagcagg taccagctgt tcagttcgta gcatctgtac
     3061 aggctcgcat caaacgcgaa gggatgcacg ctgccgccca tcacgtcatc cgcagcggga
     3121 ccaaacgccg ccgtcgaatg gcacacgtgt tcattgcgga ccactccgcg ccgccgccgg
     3181 aacacctccg tgatgcggcc cgtctcactt ggatcatggg ccacgttgaa cacgagcgtg
     3241 ggtctggcgt ggttacgctg ccacgtgaat gtgggcatca ccaccgttcc ctgcgcgccg
     3301 acagtatcca gcaacgcgtc gatcacagta tccgcgcccc cgtgtacatg cccaaacgcg
     3361 ctcagcgagg aatgcacgta cacgatctcc cccggccgga tgcccagggc gtccagcccg
     3421 cgggcgatgt cggtgcgtgt cacctgcgcg cgggggtcac tggatgtccc cattccgtta
     3481 cgctccttct ggaggcggcc cagcggcgca gggccgatgg ccgggcggtc gctttctagc
     3541 ggcggggcga tggcgacaga ggcagaagct tcgcccagcg catgagttcg tccatcgggg
     3601 caagatggcc cgcgacaaat tgcagcacga gttccatgac cccctgggca tcggccattg
     3661 agcggtgcag accgtcggtg ttcttatg
//
LOCUS       DAOPBQ010000386         3687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_20299_length_3687_cov_4.635738, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000386 DAOPBQ010000000
VERSION     DAOPBQ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20299_length_3687_cov_4.635738"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PKW60_11625"
     CDS             complement(<1..412)
                     /locus_tag="PKW60_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HPK00127.1"
                     /translation="MKRPAAAQLRHIARAVFAPRRMLLICAAAALTLAVVLVWPMDTR
                     AYLELSDSNELLDRDGRPLYVFLNSKEQWCFPRPMAEISPHLLHATVATEDKRFYSHP
                     GVDPLAIVRAALTNLRGSKVVSGASTVTMQVVKRG"
     gene            complement(409..>3687)
                     /locus_tag="PKW60_11630"
     CDS             complement(409..>3687)
                     /locus_tag="PKW60_11630"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPK00128.1"
                     /translation="KVALERRGWKPAGWEGYRFENDSKFKPDTIPVGEKQTDSNGKTT
                     FTFSHAAPPDATSPLTATVFAGVFELGGRAVYGTAQAVYFPSGPCLGLRVSKPEGGAG
                     IEASVAAVNPDGTPAELEQATVYLEKQVWNYYVRRYYSHYDSNWTESFDPITSKEVAL
                     QGGKGSVTFDLSDWGYYRLRVVSDKTKQFSTATFYSYGGQVDMVSTARPSLIKLSLDK
                     ALYTIGETATLKVESPFDGQGIVTVQGDSLQDMIPVTIVNNVGQIELPVTEAHFPNVW
                     LEVTVLHAIESGKTQMHPFSSFALTSVNVENPARKLAVAFPDLPEEVRPAGQREFTVQ
                     VSNHEGNPVEAEVTLAAVDEGIHDITDYQNPAPYEHFSRPRRPDFRRAHYYDKVAYDF
                     DKPEVGGDALRQLAKRSGAADESWIKPVALWSGVVHTGADGTARVLMDVPEFSGQLRL
                     VAVACTTSAAGASGNSVYVRRPYMLRTSLPRFLLPGDAATCRAVLFNTTDAPCTVALS
                     SAVTGPLQIAGEPKTVTVPANSEASVEIPLRATEVIGQGNIRWEAVVADAQGSEVERL
                     VQETPLPVKPPAAFQSHHELTQVAPGMSQTFKNTRFIANDQSEMEIVVSANPQTQLYE
                     SLKYLVHYPYGCAEQTTSALMPIYVLRKSQKLVEEVMKDRARLDDYIKGGIARLFAMQ
                     TASGGLGYWPGSVEPYPYGSVYGLHFLTLVQNGREFELPELPLEALKNYVRGIANDWT
                     QKHTKSNLYLRAYATYVLSLGGDLNAIQQIRRFDSITIPRSARYLLAAALARATQDKD
                     RVALYLASTPSEPYAIAERGGTLNSDIRNTAVEVLSLTQIDPASNELHDKARELIRYL
                     SSERGWNPQAEAFVASALSDYFESAAVSADQAAATIVGPNGQQTLQRAEIYRDKHEGP
                     GGEYTITNTGLAVIYVNLTTAGVPEQPDLEGYAEGITIARRYLTSRGDAYKTDAGFLQ
                     SDSYVVELTITPKEGVENVVVVDKLPAGFEIENPRLNAETLPPGVFKAATNPSYVDVR
                     DDRIVLAFNKLTHKSEGHRYYYVVRAVTPGAYQAPPATTECMYDASVRAASPMGRVEV
                     KSRQ"
BASE COUNT          678 a         1120 c         1115 g          774 t
ORIGIN      
        1 ggccccgttt gaccacctgc atcgtcacgg tcgatgcgcc cgagaccact ttgcttccgc
       61 gaaggttcgt cagagccgcc cgcactatgg ccagcggatc cacacccgga tggctgtaaa
      121 accgcttatc ctcggtcgcc accgtggcgt ggaggagatg cgggctgatc tccgccatgg
      181 gccgcggaaa acaccattgt tccttggagt tcaaaaatac atacaggggc cggccatctc
      241 ggtcgagcaa ttcgtttgaa tcggaaagct cgagataggc ccgggtgtcc atcggccaga
      301 ccagcaccac ggcaagggtc agagcggcgg ccgcgcagat caggagcatc cggcggggcg
      361 caaacaccgc ccgggcaatg tgccgcaact gtgcggctgc cggtcgtttc attgccggga
      421 tttcacctcc acgcggccca tgggactggc cgcccgtacg cttgcgtcgt acatgcactc
      481 tgtcgtcgcc ggaggcgcct gatacgcgcc cggggtcact gcgcgcacaa cgtagtaata
      541 gcggtggcct tccgacttgt gtgtgagctt gttgaaggcc agcacgatac ggtcgtcgcg
      601 gacgtcaacg taactggggt tggtggcggc tttgaatact ccggggggca gtgtttcggc
      661 gttgagccgc ggattctcga tctcgaatcc cgccgggagc ttgtccacga ccacgacgtt
      721 ttccacaccc tctttcggcg ttatcgtcag ttccacgaca tagctgtccg actgcaggaa
      781 tcccgcgtcg gtcttgtacg cgtcgcctcg actcgtcaag taacgtcgcg cgatcgtgat
      841 gccttcggcg tacccttcca ggtcgggctg ttcaggcacg ccggccgtgg ttaaattcac
      901 gtagatgacg gccagaccgg tattagtgat tgtgtattcg ccccccgggc cttcgtgctt
      961 atcacgatag atctccgctc tctgaagcgt ctgctgtccg ttcgggccga cgatcgtagc
     1021 ggcagcctga tcggcgctga cggccgcgga ttcaaagtag tcgctcaagg ctgaggcaac
     1081 aaacgcctcg gcctgcgggt tccacccacg ctcgcttgaa aggtaacgaa ttaattccct
     1141 ggccttatcg tgcaactcgt tggaggccgg atcgatttgc gtcagcgaca gaacctcgac
     1201 cgcggtgttg cggatgtccg agttgagggt gccgccgcgt tccgcgatcg catagggctc
     1261 agaaggtgtc gatgccaggt acaacgcgac ccgatccttg tcctgggtag cccgggccaa
     1321 ggccgccgcc agcaggtacc gcgctgagcg cgggatcgtg atgctgtcga aacggcgtat
     1381 ctgttggatg gcgttgagat cgccgcccag ggagagaacg tacgttgcat acgcgcgaag
     1441 gtacaggttg gatttcgtgt gtttctgagt ccaatcattt gcgatgccgc gcacataatt
     1501 cttcagagct tccaggggta gttcaggaag ctcgaattca cggccgttct gaaccagcgt
     1561 gaggaagtgg aggccgtaca ccgaaccata cgggtacggt tccacgcttc ccggccagta
     1621 ccccagaccg cccgaggccg tctgcatcgc gaacaggcgt gcaattcctc ccttaatgta
     1681 gtcgtccagc cgcgcccggt ctttcatgac ctcttcgacg agcttctggc ttttgcgcaa
     1741 tacatagatc ggcatgagtg ccgacgtggt ctgctcggca cacccgtagg ggtaatgcac
     1801 cagatatttt agcgattcgt agagctgcgt ttgtggattt gcgctgacca caatctccat
     1861 ttcagattgg tcattggcga tgaaacgggt gtttttgaag gtctgcgaca tgcccggggc
     1921 gacttgtgtc aactcatggt gtgactggaa tgcggcgggc ggtttgaccg gtaacggcgt
     1981 ttcctgcacg agacgctcca cttcgctgcc ttgcgcatcc gctacgaccg cttcccaccg
     2041 gatgttgcct tgtcctatta cttcggtggc gcgtaatggg atttcgacgc tggcttcgct
     2101 gttggcgggc acagtcaccg tctttggctc accggcaatc tgcagcgggc cggtgacggc
     2161 gctggaaaga gctaccgtac acggagcatc cgttgtgtta aacaacaccg ctctgcaggt
     2221 ggccgcgtcg ccggggagca ggaaacgggg gaggctggtc cgcagcatgt acgggcgacg
     2281 cacatacacg gaatttccgg acgcgcccgc cgccgacgtg gtgcatgcaa ccgccaccag
     2341 gcgcaattgg cccgaaaatt ccggcacatc catgagcacg cgcgcggtcc catcggcgcc
     2401 ggtgtgtact acgcccgacc acagcgcgac cggtttgatc caactttcgt cggccgctcc
     2461 ggagcgtttg gcgagttgtc gtaacgcgtc gccgcccacc tcaggcttgt cgaagtcgta
     2521 cgccaccttg tcgtaatagt gggcacggcg gaaatcgggc cggcgcgggc gcgagaaatg
     2581 ctcatacggc gccgggttct ggtaatcggt gatgtcgtga atgccttcat caaccgccgc
     2641 cagcgtcact tccgcttcaa ccggattgcc ctcatgattg gacacctgca ccgtgaattc
     2701 ccgctggccc gcgggtcgca cttcctcggg caggtcaggg aatgccacgg cgagtttccg
     2761 cgcgggattc tcgacgttca cgctagtcag cgcaaagctc gagaacggat gcatctgagt
     2821 cttgccgctt tcaatggcat gaagcaccgt cacctccagc cagacgttcg ggaaatgcgc
     2881 ttccgtcacc ggtagttcaa tctggcccac gttgttcacg atcgtcacgg ggatcatgtc
     2941 ctgcaaggaa tcgccctgga ccgtcacgat cccctggccg tcaaacggag actcaacctt
     3001 caatgtcgcg gtttctccga tcgtgtacaa cgccttatcg agcgagagct tgatcaggct
     3061 ggggcgtgct gtcgaaacca tatcaacctg gccgccgtag gaatagaacg tcgccgtgct
     3121 gaactgtttc gttttgtccg aaaccactcg caggcggtaa tacccccaat cgctcaggtc
     3181 aaacgttacc gaacccttgc cgccctgcag ggcgacctct ttcgaggtaa tcgggtcgaa
     3241 agactcggtc cagttcgaat cgtagtggct gtaatagcgc cggacgtagt aattccagac
     3301 ctgcttctcg agatatacgg tcgcctgctc aagttcagcc ggcgtgccgt cggggttgac
     3361 cgcggccaca gacgcctcaa tgccagcgcc gccttcgggt ttggatacgc gaaggcccag
     3421 acacgggccg gatggaaaat agaccgcttg ggcggtgcca tagacggcac gtccgcccag
     3481 ttcaaagacg ccagcgaaca ccgtcgccgt gagcgggctg gtggcgtcag gcggcgcggc
     3541 gtgcgaaaac gtgaacgtcg tcttgccgtt cgaatcggtc tgcttttcgc cgactgggat
     3601 cgtgtcgggt ttgaacttcg aatcgttctc gaaacgatac ccttcccagc cggcgggctt
     3661 ccagcctcgc cgctcgagcg ccacctt
//
LOCUS       DAOPBQ010000387         3661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_20498_length_3661_cov_4.104548, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000387 DAOPBQ010000000
VERSION     DAOPBQ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20498_length_3661_cov_4.104548"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..1743
                     /locus_tag="PKW60_11635"
     CDS             289..1743
                     /locus_tag="PKW60_11635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPK00129.1"
                     /translation="MKSPHRYEGLAEITRMAVPIVFGQFSYVIMQFADQVMVARLGTE
                     ELAATAPAGLWIFIFSTFFLGISGCVSTFASQCLGRGEKELGASYAWQGVYVGLLGGV
                     FGLALWPFADDLFSIMGHSPEITRLEVQYFQIRMTGFVFLCLQASLTSFFQAMNRPSV
                     PMYAAWVANVVNLVLNYLLIFGKFGFPRLEVAGAAWATVIALSLQTGILLLVFLSPAL
                     DREFKTRSQYALHGEKVRELVRIGWPAGLSFFLDVFNWGVFTSFLVGRCGETQMAAHN
                     VAINFLHLIFMPAVGLNHAIAPIVGQWIGRGDIPMAKARTYTAIKIAICYMVVAGLCF
                     ATFARPLTRTFFSTDPQVVEVAAVILLLAAFYQGFDAVSIVVLGALRGAGDTRWVMWA
                     MSIGAYLFFLPLSSFLCLDALPLSRIPGVPAGWSLPGLGWEAKGAWLGATVYTIALSG
                     VVFWRFQSEGWRHIRIFAKDRVAAPEMPVPSEEA"
     gene            1978..2799
                     /gene="rpsB"
                     /locus_tag="PKW60_11640"
     CDS             1978..2799
                     /gene="rpsB"
                     /locus_tag="PKW60_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPK00130.1"
                     /translation="MSAVSMKSLLEAGIHFGHQTRRWNPKMKKYIFGARNGIYIIDLQ
                     KTLRQLNRARAFVTEAVAKGGHVLFVGTKRQAQEIIQREAERCGMYYVNNRWLGGTLT
                     NYETIQVSIRNLRRLEEMEASGELEKFGKKEAARMRKQKARLAKYLDGIKNMPGIPRV
                     MFVIDSKREAIAVNEARRLNIPCIGIVDTNSDPDTVSFPIPGNDDAIRAVTLFCSVIA
                     DAVIEGRALSGKSVPGELPGAAAGAAKSAEAAAETEAEEAEEAVSGDDEDEDEEE"
     gene            2863..3492
                     /gene="tsf"
                     /locus_tag="PKW60_11645"
     CDS             2863..3492
                     /gene="tsf"
                     /locus_tag="PKW60_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPK00131.1"
                     /translation="MGVTAKDVKELREATGAGMMDCKKALQECGGDFDKAASWLREKG
                     IASAAKRQDREAKEGAVTSYIHMGGKIGVLVEVNCETDFVARGELFQQFCKDVCLQIC
                     STSPRWVRRDEAPQAEIDAEMVIYLAQAKESGKPEAVQHKISEGKLNKWLQENSLMEQ
                     EFVKNPDVTIEELMKALSGKVGEKIDIRRFVRFQLGEAIAGKKPSSSEE"
BASE COUNT          721 a          991 c         1241 g          708 t
ORIGIN      
        1 ctacgcaaga gaagaacgca ggcaagatgc ctgcgctacg cgctacgata accaagtggt
       61 ccggggcaga gctgcgtggc ctcccaccgg cccttcctca gccgcgcacg agccgtgccg
      121 tgacttacgg gggcctgacg tgctaggctc atactcatgt gcacgaggcc tgcgggaagc
      181 atttcgcgcg ggcgtgctac accctgcaca ccccgcaagc gtcaatgacg gccttgccgt
      241 gcataaccgc agccgttttc cctgaaacgc gcatcaggac aggagatcat gaagtccccg
      301 caccgctatg aagggctcgc cgagatcacg aggatggcgg tcccgattgt atttggacaa
      361 ttttcgtatg tgatcatgca gttcgctgac caggtgatgg tcgcccggct cggtacggaa
      421 gaattggcgg cgacggcacc ggcgggattg tggattttca ttttttcgac gttttttctc
      481 gggatatcgg gctgcgtatc gacctttgca tcgcagtgcc tgggccgggg tgagaaagaa
      541 ctcggtgcga gctatgcgtg gcagggcgtg tacgtgggcc tgctgggcgg ggtgtttggg
      601 ttggccctct ggccgtttgc cgacgacctg ttcagcatca tggggcattc accggagatc
      661 acgcgcctgg aggtgcagta ttttcagatc cggatgacgg gttttgtgtt tttgtgcctt
      721 caggcgtcgc tgacctcgtt tttccaggcg atgaaccgtc cgagcgtgcc gatgtatgcg
      781 gcgtgggtgg ccaacgtcgt gaatctcgtg ctcaattact tgctgatttt cgggaaattc
      841 ggattcccgc gcctggaagt ggcgggcgcc gcctgggcca cggtcattgc gctgagcctg
      901 cagacgggga tcctgctgct ggtctttctg tcgccggcgc tggaccggga atttaagacg
      961 cggtcccagt atgcgttgca cggcgagaag gtgcgcgagc tggtgcgtat cggttggccg
     1021 gcaggactga gttttttcct ggacgtgttc aactggggcg tattcacgag ctttctggtg
     1081 ggccgctgtg gggagacgca gatggcggcg cacaatgtgg ccatcaattt cctgcacctg
     1141 atcttcatgc cggcggtggg gctcaatcat gcgatcgccc cgatcgtagg gcaatggata
     1201 gggcgcggcg acattcccat ggccaaggcg cgaacgtaca cggcgatcaa gatagccatc
     1261 tgttatatgg tcgtggcggg cctatgcttt gcaacattcg cccggccatt gacacgcacc
     1321 ttcttcagta cggatccgca ggtggtcgag gtggcggcag tcatcctgtt gctggcggcg
     1381 ttttaccagg gatttgacgc ggtgagcatc gtggtgctgg gcgctctgcg gggcgcgggc
     1441 gacacgcggt gggtcatgtg ggcgatgtcg atcggggcgt atctgttctt tttgccgctt
     1501 tcgtcgtttc tgtgtctgga cgccctgccg ctctcccgga taccgggcgt tccggcgggc
     1561 tggtcgcttc cggggttggg atgggaagcc aaaggggcct ggctgggcgc cacggtgtat
     1621 acaatcgcgt tgagcggcgt ggttttctgg cgctttcaaa gcgaaggctg gcggcacatc
     1681 cggatttttg cgaaagaccg tgttgcggct cctgagatgc ccgtgccgag cgaggaagcg
     1741 taatggcgcc gtgaccggcc gggaaacggc ccggctcctg cccgttcgcc cgggccgcac
     1801 gcggtaatcc cgcgctatgc ctatagtttg cgccgttgaa ggcggatatg ctagactttc
     1861 tcgattccaa attcacaggg gtttcgagtc tgtgtaccgc tgtggtccgg gtgcctggcc
     1921 gcttggccgg gtagaccgca ggaaaccccg agggtcaacc cagaaggagc gtgttccatg
     1981 tctgcagttt ccatgaaaag ccttctcgag gccggcattc actttggcca ccaaacccgc
     2041 cgttggaatc cgaagatgaa gaaatacatc ttcggcgcgc gcaacggcat ctacatcatt
     2101 gacttgcaga agaccctgcg ccagctcaac cgggcccgcg cgttcgtgac cgaggccgtg
     2161 gccaagggcg gccatgtgtt gttcgtcggc acgaaacggc aggcgcagga gatcatccag
     2221 cgcgaagccg agcgctgcgg catgtattac gtgaacaacc ggtggctggg cggcacgctg
     2281 accaattacg agacgattca ggtgagcatc cgcaatctcc ggcggctgga agagatggaa
     2341 gccagcggcg agctggagaa attcggcaag aaggaagcgg cgcggatgcg aaagcaaaag
     2401 gcccgccttg cgaaatacct ggacggcatc aagaacatgc cgggcatccc gagagtgatg
     2461 ttcgtcatcg attcgaaacg ggaagccatc gcggtgaacg aagcgcgccg tctgaacatc
     2521 ccgtgcatcg gcatcgtcga cacgaacagc gaccccgaca cggtgtcgtt cccgattccg
     2581 ggcaatgacg acgccatccg ggcggttacc ctcttctgtt ccgtgatagc ggacgcggtg
     2641 atcgaaggcc gtgcgctgtc cggcaaatcc gttccgggag aattgccggg agcagctgcc
     2701 ggagcggcga agtccgccga agcggcggcc gaaacggaag ccgaggaagc agaagaagcg
     2761 gtcagcggcg acgacgagga cgaggacgag gaagaatagg cgcgcatacg ggcaggcgca
     2821 tgtggctcag cacgggaaca tcgtaaagga gatagtgaga ttatgggcgt tacggcgaaa
     2881 gacgtgaaag aactccgcga agcgaccggc gccggcatga tggattgcaa gaaagcgctc
     2941 caggaatgcg gcggcgattt cgataaggcg gccagctggc tgcgtgagaa agggattgcc
     3001 tcggcagcaa agcgccagga tcgggaagcg aaagaaggcg cggtgacttc gtacatccac
     3061 atgggcggga agatcggcgt actggtcgaa gtaaactgcg agacggattt cgtggcgcgc
     3121 ggcgaactgt ttcagcagtt ctgcaaagac gtgtgccttc agatctgctc cacgtcgccg
     3181 cggtgggtgc gccgggatga ggcgccgcaa gcggaaattg acgcggaaat ggtcatttac
     3241 ctcgcgcagg cgaaggaaag cggcaaaccc gaggccgtac agcacaaaat ctccgaaggc
     3301 aagctcaaca agtggctgca ggaaaacagc ctcatggagc aggaattcgt caagaacccc
     3361 gatgtgacca tcgaggagct gatgaaagcc ctgagcggca aggtcgggga aaaaatcgat
     3421 atccggcggt ttgtccggtt ccagttgggc gaagcgattg caggtaagaa accgtcgtcc
     3481 agcgaggagt aagccggatg cacggctcag cgtaccagcg cgtcctgttg aaactgagcg
     3541 gccaggccct ggagccgagc cgtggcgcgg ggatcgattt ccgtacggtt gaccagtttt
     3601 gcgacgagat cgccaaagcg cacgccctgg gcgtggaact cggcctggtg gtcggcggcg
     3661 g
//
LOCUS       DAOPBQ010000388         3600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_20884_length_3600_cov_3.612976, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000388 DAOPBQ010000000
VERSION     DAOPBQ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20884_length_3600_cov_3.612976"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PKW60_11650"
     CDS             <1..729
                     /locus_tag="PKW60_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPK00132.1"
                     /translation="LAATGEATVQQLAARFAVSVMTIRRDLAALEAQGELTRIHGGAM
                     LTRAGVIEFSFKEKAERNADEKRGIAREAARLVSSGMTLILDTGTTTLEVARAIADIP
                     RLTVLTSSLAIASALYGRQNITLVLAGGAVRTGSPDLSGWLTEENMKRFRVDIACIGA
                     DGADRDGASTTDTDVARVSQAMIASARETILVADHTKFEKPAFVRFASWKDIDRVITG
                     STMAADTRAWLEGAVKELTYAEHA"
     gene            713..2194
                     /locus_tag="PKW60_11655"
     CDS             713..2194
                     /locus_tag="PKW60_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPK00133.1"
                     /translation="MPNTRKRRSAPGRLLIGIDLGTTVLKACAFDADSGDVAAKEARR
                     LSVKLLANGGSEQNIASLDRTVSGVLASLRKQLGHRWAEVAGFGVAAQGGSSIIADRN
                     TGAALTPMVLWNDTRAHGQIAEIAERSTPEFWRRFTLRDGVPHGLGRLAWLQERAPAL
                     FTDAHIHIGAGEWLFHRLTGLWRQDPGNAIQTGAYNAAAQRLDPAAFALIGLDLPFVA
                     PLRRDHETAPLCGAAARRLGLPPGIPVAGPYIDQEAGYLSTVEASSPPVHCSLGTAWV
                     CNYTRPESLMLDSPAQLVLGGITGAGQLVVLPLRTGNPAWDWALRTFLDPDLPKAFRK
                     AETIFRSCLVPPAGLVAVPHLAQRNPFFPEAFGAGSFWGIHTAATTSDLVRAVAAGMV
                     FELAEALQPIVAGRLAERIVAGGGASKAGYLCCLIAALFPEGSVQRQREADWAVARGA
                     LYALAPAVAHAPVETIRKPRAAVCDAAQESANHYARVRESLNL"
     gene            2218..>3600
                     /locus_tag="PKW60_11660"
     CDS             2218..>3600
                     /locus_tag="PKW60_11660"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00134.1"
                     /translation="MQQPKAGLLPLYLKLYDDVDPALRASFKPFLDTIAQGLQERGVS
                     VSLGEICRVNEEFTKTVKRFEQEQVDLIVTLHLAYSPSLESAGILAASRLPLLLLDTT
                     PDASFGPGTSPDRIMFNHGIHGVQDLASVLRRMGRHFEIVAGHHANPEVLDRAAGIAR
                     AAQAARYLRTTRALRIGPAFEGMGDFAPPDAVLERVLGIRTDQIDVEALVAEAAFITE
                     TDLQEEMEADRARYAVEASEEVHRRSLRSGLALRKRLTDGAYTAFSLNFLEFRAADGL
                     IDTVPFLEVSKAMARGYGYAGEGDVLTASLVGALNKAFGKTTFTEIFCPDWEGDSLFL
                     SHMGEVNPEVAGAVPRLIEKDFPYTPAQNPAILACGLHPGPAVLVNLAPGPGDSFGLL
                     VAPVQVLEDTTVPEMKDAVRGWIRPELPVELFLEEYSINGGTHHSALVMGECAGDIVA
                     FGSMTGLDVTT"
BASE COUNT          645 a         1140 c         1168 g          647 t
ORIGIN      
        1 ctggccgcca cgggtgaagc cacggttcag caactggcgg cccggttcgc ggtgagcgtc
       61 atgacgatcc ggcgtgacct ggcggcgctc gaggcccagg gcgaactcac gcgaatacat
      121 ggcggcgcca tgctgacccg ggctggcgtc atcgaatttt catttaaaga aaaggcggag
      181 cgcaacgccg atgaaaaacg tggtatcgcg cgcgaagcgg cgcgtctggt gagcagcggc
      241 atgaccctga tcctggacac cggaaccacc acgctggaag tggcgcgggc cattgcagat
      301 attccccgcc tgaccgtgct tacctcatcc ctggcgatcg cctcggccct gtacgggcgg
      361 caaaatatca cgctggttct tgccggcggc gcggtgcgca cggggagtcc tgacctgtcg
      421 ggctggctta ccgaggaaaa catgaaacgc ttccgggtcg atatcgcctg catcggcgca
      481 gacggcgcgg atcgggacgg cgcatccacc acagacacgg acgtagcccg ggtatcccag
      541 gccatgatcg ccagcgcgcg ggaaaccatt ctggtcgcgg atcatacaaa atttgagaag
      601 ccggccttcg tccgtttcgc ctcgtggaaa gacatcgacc gtgtgatcac cggctccacc
      661 atggccgccg acacccgcgc ctggctggaa ggcgctgtca aggagctgac ctatgccgaa
      721 cacgcgtaaa cggcgcagcg ccccaggccg cctgctcatc ggcatcgatc tgggcacgac
      781 cgtgctcaaa gcctgcgcgt ttgatgcgga ttcaggggat gtggccgcga aggaagcccg
      841 gcgcctctct gtcaagcttc tcgccaacgg cggatccgaa caaaacatcg cttcgctcga
      901 tcggactgta tctggcgttc tggcttcgct tcgaaaacag ttgggccacc gctgggcgga
      961 ggtagcagga ttcggcgtgg cagcccaggg cggcagcagc atcattgccg atcgcaacac
     1021 gggcgcagcg ctcactccca tggtgctgtg gaacgacacc cgggcgcacg ggcagatcgc
     1081 cgagattgcg gaacgcagca cgccggaatt ctggcggcga ttcaccctgc gcgatggcgt
     1141 accgcatgga ctgggccggc tcgcctggtt gcaggagcgt gcgccggcct tgttcacgga
     1201 tgcccatatc cacattggcg cgggcgaatg gctttttcac cgcttgaccg gcctctggcg
     1261 gcaggatccg ggaaatgcca tccagaccgg agcctacaac gccgctgccc aacgcctcga
     1321 ccccgctgct tttgctttga tcggcctgga tctgccgttc gtcgcaccgt tgcgcagaga
     1381 ccacgagacc gctccactct gcggcgcagc ggcgcgacgg ctcggcttgc caccgggcat
     1441 tcccgtagcg ggaccgtaca tcgaccagga agcggggtat ctgtccacgg tggaggcgtc
     1501 gagccctccg gtgcactgtt ccctgggtac ggcctgggtt tgtaactaca cgcgaccgga
     1561 aagcctgatg ctcgactcgc cggcacaact ggtgcttggc gggatcacgg gcgcagggca
     1621 attggttgtg cttccgttgc ggacgggcaa tccggcgtgg gattgggccc tcaggacatt
     1681 tttggatccg gatctcccga aagcgtttcg caaagcggag actattttcc gctcctgtct
     1741 tgttcctccg gcaggcctgg tggcggtgcc tcatctcgcc cagcgcaacc cgtttttccc
     1801 cgaggccttc ggggccggga gcttctgggg cattcacacg gccgccacca ccagcgacct
     1861 cgtgcgcgct gtggcggcag gcatggtttt tgagcttgcg gaagcattgc agcccatcgt
     1921 ggccggaagg ttggcggagc gcatcgtggc cggaggcggc gcaagcaagg ccggttatct
     1981 ctgctgcctg atcgccgcgc tgtttccgga agggtcggtt caacggcagc gtgaagcgga
     2041 ctgggccgtg gcgcgcggcg cgctctatgc tctggcgccg gccgttgccc acgccccggt
     2101 cgaaaccata cggaaacccc gcgctgcggt ctgtgacgct gcacaggaat ccgcaaacca
     2161 ctacgcgcgc gttcgagaga gtctcaacct atagtgaatg aaatggaggc aacgctgatg
     2221 caacagccga aagccgggct cctgcccctt tatctaaagc tctacgacga cgtggaccct
     2281 gcgctgcggg cctcgttcaa accatttctg gacaccattg cccagggctt gcaggaacgc
     2341 ggcgtatcgg tctcgctcgg cgagatctgc cgcgtaaacg aagaattcac caaaacagtc
     2401 aagcgtttcg agcaggaaca ggtcgatctg atcgtaacgc tccatctcgc gtactcgccg
     2461 tccctggaat cggccgggat cctggccgcc agcagactcc ccctgctcct gctcgatacg
     2521 acgccggatg catcgttcgg gccggggaca tcgccggacc ggatcatgtt caaccacggg
     2581 atccacgggg tgcaggatct ggcgtccgtg ttgcgcagga tgggcaggca tttcgagatc
     2641 gtggcggggc accatgccaa tccggaagtg ctggaccgtg cggccggcat tgcgcgggcg
     2701 gcccaggcag cacgctatct gcggacaaca cgcgccctgc gcatcggccc cgcgtttgaa
     2761 ggcatgggcg attttgcgcc gccggacgcc gtccttgaac gcgtgctggg aatcaggacc
     2821 gaccagatcg acgtcgaagc cctggtggcg gaagcggcct tcatcacgga aacagacctc
     2881 caggaagaga tggaagcgga tcgcgcccgc tacgccgtcg aagcctcgga agaggtgcac
     2941 cgccggtcgc tgcgctccgg tttggcgctg cgtaaacgcc tgacggatgg cgcctacacc
     3001 gcattcagtc tgaatttcct ggaattccgg gccgccgatg gcctgataga caccgtgccc
     3061 ttcctggaag tctcgaaggc gatggcgcgt ggttacgggt atgcgggcga gggcgatgtc
     3121 ctgaccgcgt cgctggtggg cgcgctgaac aaggccttcg gcaaaacgac gtttactgaa
     3181 attttctgcc ctgactggga aggtgactcg ctgttcctgt cgcacatggg cgaggtgaat
     3241 ccggaagtcg ccggagctgt gccgcggctg attgaaaaag actttcccta cacgcccgcg
     3301 caaaatccgg ccatcctggc gtgcggattg catccgggcc cggcggtact ggtgaacctc
     3361 gcccccggcc ccggcgatag cttcgggctg ctcgtcgctc cggtccaggt cttggaggac
     3421 acgacggtac cggagatgaa ggatgccgtc cgcggatgga tccgccctga actcccggtc
     3481 gaactctttc tggaggagta ctccatcaac ggcggcacgc atcacagcgc cctggtgatg
     3541 ggcgagtgcg ccggcgacat cgtcgcgttc ggttcgatga caggactgga cgtcacaacc
//
LOCUS       DAOPBQ010000389         3585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21006_length_3585_cov_3.545326, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000389 DAOPBQ010000000
VERSION     DAOPBQ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21006_length_3585_cov_3.545326"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /gene="ribD"
                     /locus_tag="PKW60_11665"
     CDS             <1..1049
                     /gene="ribD"
                     /locus_tag="PKW60_11665"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPK00135.1"
                     /translation="ELAARGRGRTSPNPMVGCVIVRDGAVIGEGYHARAGEPHAEVLA
                     VEAAQGDLTGATVYVSLEPCSHVGRTPPCADLLLEKRPARIVVAMSDPNPRVSGEGIR
                     RLRQAGLEVDVGVLEDEARALNEAFVKYITTGMPFVICKAAMTLDGKIATHTGHSRWV
                     TSEESRRRVHELRSCVDAILVGSRTVMLDDPSLTTRLESGEGRDPIRIILDADDYLDE
                     KRRVFEVESPAPTWVVLPEDRGCDRADEVLHVRRGGDGFDLRHLMALLGEREITSVLI
                     EGGGTTHASAFEAGIVDKVMFFVAPKIIGGRDAITAVEGEGVATMDEAVLLERMTAER
                     VGDDLLIEAYVKAR"
     gene            1054..1677
                     /locus_tag="PKW60_11670"
     CDS             1054..1677
                     /locus_tag="PKW60_11670"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPK00136.1"
                     /translation="MFTGIVEEVGAISRKSGAELSILAERVLDGMRHGDSIAVNGVCL
                     TVTAFNERGFTAQISPETIGRTTLGSARPGDAVNLERALSMGDRFGGHFVQGHVDGVG
                     RVYSLSNQGEFSLWRFRAPDDVARYLVPKGSVAIDGISLTVVEPAGDTFGVAIIPETL
                     KRTILGAKRPGDPVNLEADVIGKHIYHYVKAGHNGLTQDFLARHGFA"
     gene            1696..2889
                     /locus_tag="PKW60_11675"
     CDS             1696..2889
                     /locus_tag="PKW60_11675"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPK00137.1"
                     /translation="MFATIPEILDELRAGKIIILVDDEDRENEGDFVIAAEKVTPEAI
                     NFMITHGRGLVCLAMTGQRLEELEIPPMTSRNRSRFGTAFHVSIEAAEGISTGISPYD
                     RARTVQVAIDPETKPGDLVQPGHIFPLRARTGGVLVRAGQTEGSVDLTRIAGMMPAAV
                     ICEVINADGTMARVPQLAEIAREFDLKMCSVESIIQWRRRNEQLVHRAAQTRLNTEHG
                     VFDLIVYDNDVDKREHVALVMGDVATEEPVLVRVHSECLTGDVFGSLRCDCGEQLHEA
                     MRMIEQEGRGVIVYMRQEGRGIGLMNKIRAYELQDNGLDTVEANVHLGFPPDPREYGI
                     GAQILSDLGVRRMRLLTNNPVKRAGLEGYGLEVVRLQPIQIPPNEINARYLQTKRDKL
                     GHLLS"
     gene            2900..3367
                     /gene="ribE"
                     /locus_tag="PKW60_11680"
     CDS             2900..3367
                     /gene="ribE"
                     /locus_tag="PKW60_11680"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPK00138.1"
                     /translation="MMPKTIEGKLVSSGKKYGIIVSRFNEFISGKLLAGALDALQRHG
                     VLDEEITVAWTPGSFEIPMVARKMALSGHYDAVIALGAVIRGSTPHFDYVAAEVSKGV
                     AHVTLDTLVPVAFGVLTTDTIEQAVERAGTKSGNKGFDAACSAIEMANLLEQL"
     gene            3364..>3585
                     /locus_tag="PKW60_11685"
     CDS             3364..>3585
                     /locus_tag="PKW60_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00139.1"
                     /translation="MKGFNRKNRRSGRECAVQFLYGLDFTEYDWESALDDFWASMTGI
                     PGDCAAVGEEARAYAAGLIEGIMLHRDALD"
BASE COUNT          679 a         1002 c         1227 g          677 t
ORIGIN      
        1 tggaacttgc agcgcgaggc cggggccgca cgtcgcccaa tccgatggtg ggctgtgtca
       61 ttgtccggga tggcgcggtc atcggtgaag gctaccatgc gcgcgcgggc gagccgcatg
      121 cggaggtcct ggccgtcgag gcggcgcagg gcgatctgac tggagcgacg gtgtacgtca
      181 gcctggagcc ttgctcgcac gtcggccgga cgcctccctg tgcagacctg ctcctcgaga
      241 aacggccggc ccggatcgtg gtggccatga gcgatcccaa ccccagggta tcgggggaag
      301 gcatccgccg cctgcgccag gccgggcttg aggtcgacgt cggtgtgctg gaggatgagg
      361 cccgcgcgct caacgaagct tttgtcaaat atatcaccac gggcatgccg ttcgtgatct
      421 gcaaagccgc gatgactctg gacggcaaga tcgcaaccca caccgggcat tcgcgctggg
      481 tgaccagtga agaatccagg cggcgggttc acgagcttcg cagttgcgtc gatgccatcc
      541 tcgtcggcag ccgtacggtc atgttggacg atccgagcct gaccacccgg ctggagagtg
      601 gggaagggcg cgaccccatc cggattatcc tcgatgccga cgactatctg gatgaaaagc
      661 gccgcgtgtt tgaagtcgaa agcccggccc cgacgtgggt ggtgttgccg gaagatcggg
      721 gctgcgaccg ggcggatgag gtgctgcatg tgcggcgcgg cggcgatggg ttcgatttgc
      781 ggcatctcat ggcgctgctg ggcgaacgcg agatcacgtc ggtcttgatc gaaggcggcg
      841 gaacaacgca tgcctccgcg ttcgaggcgg gcattgtgga taaggtgatg ttttttgtgg
      901 cgccgaagat catcggcggg cgggatgcga tcacggccgt ggaaggtgaa ggcgtggcca
      961 ccatggatga agcggtactg ttggaacgta tgacggcgga acgcgtgggc gatgacttgc
     1021 tcattgaagc ctatgtaaag gcaaggtaac cccatgttca cgggaatagt cgaagaagtt
     1081 ggtgccattt ccagaaagtc cggcgctgaa ctgtcgatcc tggcggagcg ggtcctcgac
     1141 gggatgcgcc acggggacag catcgcggtg aatggggttt gtctgacggt gacggcattc
     1201 aacgagcgcg gtttcacggc ccagatctcg ccggagacca ttgggcgtac aacgctgggc
     1261 agtgcccgtc cgggcgatgc ggtcaatctc gaacgcgccc tgtccatggg cgaccggttc
     1321 ggcggccatt ttgtacaggg gcatgtggat ggcgttggcc gggtctattc gctaagcaac
     1381 cagggcgaat tttcgttgtg gcggtttcgc gcgccggacg atgtggcccg ctatcttgtg
     1441 ccgaaagggt ctgtcgcgat cgacggcatc agcctgaccg ttgtggaacc ggcaggcgat
     1501 acctttggtg tggcgatcat cccggaaacg ctcaaacgga ctatcctggg cgcgaagcgg
     1561 ccgggcgatc ccgtgaatct tgaggccgat gttatcggca agcatattta ccattatgta
     1621 aaggccggcc acaatggatt gacgcaggat ttccttgcgc gccatggatt tgcgtgaagc
     1681 cggcaggagt gtcccatgtt tgcaaccatt ccagaaatac tcgacgagct tcgggccggc
     1741 aagatcatca tcctcgtcga tgacgaggac cgcgagaacg aaggcgactt cgttattgcg
     1801 gccgagaagg tgacgcccga agccatcaac ttcatgatca cgcatggccg gggcctggtc
     1861 tgccttgcga tgaccggcca gcggctggag gaattggaga ttcctcccat gaccagccgg
     1921 aaccgctcgc gattcggcac ggcattccac gtgtccatcg aggcggcaga gggcatcagc
     1981 accggcatca gcccctacga tcgcgcgcgc acggtacaag tggccatcga cccggagacc
     2041 aaaccgggtg acttggtcca gcccggacat atctttccgc tgcgtgcccg tacgggcggc
     2101 gtcctggtgc gggccggcca gaccgagggg tccgttgacc tcacccgtat tgcggggatg
     2161 atgccggccg cggtcatctg cgaggtgatc aacgcggatg gcacaatggc ccgggtcccg
     2221 cagcttgccg agatcgcccg ggaattcgat ctcaagatgt gctcggtgga gagcatcatt
     2281 cagtggcgcc ggcgcaacga gcagcttgtg caccgggcag ctcagacgcg gcttaacacc
     2341 gaacacggcg tgtttgacct gattgtctac gacaacgacg tggataagcg tgagcatgtg
     2401 gcgctggtca tgggcgacgt ggccaccgaa gagcccgtgc tcgtgcgcgt gcattccgag
     2461 tgcctgacgg gagacgtgtt cggctcgctc cgctgcgact gcggtgaaca gctgcacgag
     2521 gccatgcgca tgatcgagca ggaaggccgc ggcgtcatcg tgtacatgcg ccaggaaggc
     2581 cgcgggatcg gcctcatgaa caaaattcgc gcgtatgaac tgcaggacaa cggccttgat
     2641 accgtggaag ccaacgtgca cctgggtttt cccccggatc cgcgtgaata cggtatcggg
     2701 gcgcagattt tgagtgattt gggtgtgcgc cgtatgcgcc tgctgaccaa caacccggtt
     2761 aaacgggcgg ggctcgaagg ctatggcctg gaggtggtac gcctgcagcc catccagatc
     2821 cctccgaatg aaatcaatgc acggtatctt cagacgaaaa gagacaagct cggccatttg
     2881 ctgtcgtgaa aggagtgctt tgatgccgaa gactattgaa ggcaagctgg tatcgtcggg
     2941 caaaaagtac gggatcatcg tgtcgcgctt caacgagttc atctcgggca agctgctggc
     3001 gggcgcgctt gacgcgttgc agcgccatgg cgtgctggat gaagaaatca cggtggcctg
     3061 gacgccgggc tcgtttgaaa tcccgatggt ggcccggaag atggccctgt cgggtcatta
     3121 cgatgctgtg attgccctgg gcgcggtaat ccgcggcagc acgccccatt tcgactacgt
     3181 cgctgcggag gtgtcgaaag gggtggccca cgtgacgctc gacaccttgg tgcccgtggc
     3241 gttcggcgtg ctgacgaccg ataccattga acaggccgtg gaacgcgccg gtaccaagtc
     3301 cggcaacaag gggtttgacg ccgcgtgctc cgccattgag atggccaacc tgctggagca
     3361 actgtgaagg gtttcaaccg caagaaccgc cgttcggggc gcgaatgcgc ggtacagttc
     3421 ctgtacggcc tcgattttac cgaatacgac tgggaatcgg ccctggacga tttctgggcc
     3481 tcgatgaccg ggataccggg cgattgcgcg gcggtcggcg aggaggcgcg agcctatgcg
     3541 gccggactga tcgagggtat catgttgcat cgcgacgcgc tggat
//
LOCUS       DAOPBQ010000390         3583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21013_length_3583_cov_4.870465, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000390 DAOPBQ010000000
VERSION     DAOPBQ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21013_length_3583_cov_4.870465"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..998
                     /locus_tag="PKW60_11690"
     CDS             180..998
                     /locus_tag="PKW60_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPK00140.1"
                     /translation="MGLRINTNIAAINASRTLRGTTRQLNTTLERLASGLRINRAADD
                     AAGLAISEGFRTVIRGSAVAQRNAQDGISLVQTAEGALSEATNILQRMRELAVQAANG
                     TQSAANRESLDLEVRQLLEQLDDIAFDTEFNGLAVLSSAQTITLQAGFRAGQTIEIAV
                     AGASTANLGVTSVSLSTMAGAVSALGILDAAIQSVSQLRATFGAVQNRLEFTMNSLAI
                     TEENASASDSALRDADIATETIQFTRTQILISSGTAVLAQANLVPQTALQLLGG"
     gene            1729..2544
                     /locus_tag="PKW60_11695"
     CDS             1729..2544
                     /locus_tag="PKW60_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPK00141.1"
                     /translation="MGLRINTNTAAINASRVLARSSRQLNKALEQLSSGLRINRASDD
                     AAGLAIAEGFNSVVRGTRVAQRNAQDGVSLMQTAEGALSETTNILQRMRELAVQSANG
                     TMTADNRAALQAEVTQLMGQINSIASDTEFNGLRVLSAAQTITLQAGAQAGQTLELQL
                     SGAKAADIGVSSVSISTQAAAVSALSILDLAINSVSSLRATLGAFQNRLEFTINTLAI
                     QEENSAAAESAIRDADFAKVTTEYTRTQILISAGTSVLAQANVVPQTALQLLG"
     gene            complement(2691..>3583)
                     /locus_tag="PKW60_11700"
     CDS             complement(2691..>3583)
                     /locus_tag="PKW60_11700"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK00142.1"
                     /translation="AGAGDASVLLSTDADTFVEPNYLESVRRYFARPEAWAACVGYAH
                     RLDGTDAEIAAVVAYETHLRCHVLGLQLAGSPYAYPAVGSTIVCSARAYAAAGGMNRR
                     QAGEDFYFLQQLAKTGRVESIHATTVHPAPRASHRVPFGTGRWIQERLDDRQELMTYH
                     PESYRILGDLLALVNARPEADPDSILAELARISRPATEFLEKQKFAECWQKLHRNSPD
                     SRAFLAQFHRWMDAFKTLKLLHCLRDNGFPQQPLWTAAKALLERAEKMPPAFPWQNLD
                     TERKVQIALLRHLRKLERLE"
BASE COUNT          727 a         1129 c         1099 g          628 t
ORIGIN      
        1 cggccattca acaaaaggtg gtctcccggg cgggtacaca ccgcccgccg ggcttcggcg
       61 agaatccgcg gactaaagat ctccccgctt gctgccgata gactgcccgg aaccgggaaa
      121 accggcaccc aagccgatgc atggacgcag aggcggaaat atcacggagg atatgagcca
      181 tgggtcttcg gatcaacacc aacattgcgg cgatcaatgc atcccgcacc ctgcgcggaa
      241 ccacgcgcca actcaacacc accctcgaac gcctggccag cggtctgcga attaatcgcg
      301 ccgccgacga tgccgccggt cttgcgatct ccgaagggtt ccgcacggtc atccgcgggt
      361 ccgcagtggc ccagcgcaat gcacaggatg gtatcagcct cgtgcagacc gcggaaggcg
      421 cgctgagcga ggccaccaac atcctgcaac gcatgcgcga actggcggtg caggcagcca
      481 acggcaccca gagcgcggcc aaccgggagt cgctggacct cgaagtgagg cagctgctgg
      541 aacagctcga cgacatcgca tttgacaccg aattcaacgg gctcgccgtg ctgagctccg
      601 cccagaccat tacattgcag gccggattcc gcgcgggcca gaccattgag atcgcggttg
      661 ccggcgcatc cacggccaac ctgggagtca cctcggtctc cctgtctacc atggccggcg
      721 cggtgtctgc cctgggtatc ctggacgccg cgattcaaag cgtcagccag ctccgtgcga
      781 catttggcgc ggtgcagaac aggcttgagt tcaccatgaa cagcctggcg atcaccgaag
      841 aaaacgcttc ggcatccgac agcgccctcc gcgacgcgga tattgctacc gagaccattc
      901 aattcacccg cacccagatt ctgatcagtt cgggcacggc ggtgcttgct caggccaatc
      961 tggtgccgca aaccgccctc cagctgctcg gcggataagc cgcccgggtc agaaaactgc
     1021 tcatatctct ctgaactctg cacgccgcgc ctcgaaaggc gcggcttttt tcatgcccgc
     1081 ccacctaaga taaatccttt acgaaaaaag gtttagctga gaaacctgca gccccttgcg
     1141 ccccggttac tccgcataca ccaaaaaaat ccggcgattt caaaaaaagc gctaaagtgt
     1201 ttcgctaaag gtgccgatat atttgacgac aacggcggaa tgtggctgcg aaacatgacc
     1261 cgccagtgtg aggggggatt ccccagggag gcggcccgcg cagcgcgcgt gcgtcgcagg
     1321 tttgccctaa catcgtccgc aggatccagg gcgtgtaggg ccaaagacag ttgaacgaga
     1381 ccggacaaag gccggagcca ctgcgcttca tgccttggtc agttgaagac aggcagcact
     1441 gagttgccgg ggagaggcca tgcgaagacc tcacgggacc cctggaaacg ttttcggctg
     1501 ccgtggcggc gtccccaggg acaggagacg cctccggtcg tttgctggcc ggggagcttt
     1561 gccggtcgca gcccgccggt tggctgccca tagccggccc ggagttgtgc cgggacgttt
     1621 tcgcatgatg ttcgggatgg tgtttgggac acgctgtttc gaagagagcg tcgtctggca
     1681 ttgcggtgcg catggatgcg ccagattatc actcaaggag gatagaccat gggactcagg
     1741 atcaatacga atacggcggc tatcaacgcg tcacgtgtgt tagcccgctc ctccaggcaa
     1801 ctcaacaaag cgctcgaaca gctttcgagc gggcttcgca ttaaccgggc cagtgacgac
     1861 gctgcgggtc tggctattgc ggaggggttc aattcggtcg ttcgaggcac gcgggtggct
     1921 cagcgcaatg cgcaggacgg cgtcagtctg atgcagactg ccgagggcgc cctcagcgaa
     1981 acgacgaaca tcctgcagcg tatgcgcgaa ctggcggtgc aatcggccaa cggcacgatg
     2041 acggcagaca accgcgcggc gttgcaagcc gaagtcaccc agttgatggg gcagatcaac
     2101 agcattgctt cggatacgga atttaacggg cttcgcgtcc tcagcgcagc tcagaccatc
     2161 accctgcagg caggggctca ggctggtcag accctggagc tccagctcag cggcgccaaa
     2221 gctgccgaca tcggtgtcag ttcggtgagc atctcgactc aggcggcggc ggtgtccgcg
     2281 ctcagtatcc tggatctcgc tatcaacagc gtttcgtcgc tgcgcgcgac cctgggcgcc
     2341 ttccagaacc ggctcgagtt caccatcaac accctggcca tccaggagga gaactcggcg
     2401 gcggccgaaa gcgctatccg cgatgccgac ttcgccaagg tgacgacgga gtacacacgg
     2461 acgcagatcc tcatcagcgc aggcacctcc gtgctggctc aggccaacgt ggtgccgcag
     2521 accgcgctgc agctgctcgg gtaggtcacg ccccgatcgt tcgagtcacg tgagataaag
     2581 cgtcgagcgg cccggcaaca ggttccggca cagaccgggg cctctgccgg gccgttctat
     2641 atttttccgg cccggcatgt gcggctgcag caccgcgttc ccggcaggcg tcattccaaa
     2701 cgctccagtt ttctcagatg gcgcagcagt gctatctgga cttttcgctc agtatccagg
     2761 ttctgccagg gaaatgccgg tggcatcttc tccgcgcgct ccagaagggc ctttgccgcc
     2821 gtccacaacg gctgctgcgg gaatccgttg tcgcgcaggc aatgcaagag cttgagcgtc
     2881 ttgaacgcgt ccatccagcg gtgaaactgg gccagaaacg ctcgcgaatc cgggctgttt
     2941 cgatgcaatt tctgccagca ttccgcgaac ttctgttttt cgagaaattc ggtcgcagga
     3001 cgactgatcc gcgccagttc tgccaggatc gagtcggggt cggcctcggg acgagcattc
     3061 accagcgcca gcaaatcccc aagaatacgg tagctctcgg gatgataggt catgagctcc
     3121 tgtctgtcat cgaggcgttc ctgaatccac cgccccgtcc caaaaggtac gcgatgggaa
     3181 gcgcgcggcg ctgggtggac ggtcgtggcg tggatcgact cgacgcggcc cgtcttggcc
     3241 agctgctgca gaaaataaaa gtcttcgccc gcctggcggc ggttcatccc tcccgcggcg
     3301 gcgtacgccc gtgccgaaca gacaatggtc gaccctacgg ccgggtacgc atacggggaa
     3361 cccgcgagct ggaggcccag gacatggcag cgcaaatgtg tctcgtaggc cacaaccgcg
     3421 gcaatctccg catcggtgcc gtcgaggcga tgtgcataac cgacacaggc cgcccaggcc
     3481 tccgggcgtg cgaaatagcg ccgcacggat tccaggtagt tcggttcaac gaaggtatcg
     3541 gcgtcggtgg acaacaggac ggaagcatcg cccgccccgg ctt
//
LOCUS       DAOPBQ010000391         3581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21034_length_3581_cov_3.476177, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000391 DAOPBQ010000000
VERSION     DAOPBQ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21034_length_3581_cov_3.476177"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1730
                     /locus_tag="PKW60_11705"
     CDS             <1..1730
                     /locus_tag="PKW60_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPK00143.1"
                     /translation="TVSQKSYGLHAGNISGRLHLEDRSVQIQPLSFVFQDNAVVGGLA
                     YDFASKTGSASINGAIAKLEDVHLIAIKDVAVSGGATVKASAEFRPERTRAEFLAELT
                     QAQVDYRWFFRKPAGLGVTLHGTADFKPGQEIALNGQVKAADTLLDAEMLLARSGGKW
                     SLRRSLTTADTADIATIAKCLRLPYKPQGSAIRRVSYEWNRDPEEPTAWRALLEGHLD
                     ELSLLPDGLETPHVLKDAQLRLAFTRGTPSTGELWLHAASAATPSFGAQWFNPITCPP
                     ELIDRFPPVPRDWVFHLEAEHLLAPPWEASAFSGEAYTTAARIGVRQFRARVGDGRVE
                     GSYTLTRAENAYSTTFAWENIPATPLLSHLKFPNVLEGSVDGNITYSMDRDDPHTLAG
                     EGRFTVRDGRFNADYLLAQFKGPLQESAGILPPSLQFNRLASNVSFQNDVVTTKDVEL
                     LAEGINVTGHGTYVTGGDMDYAIRLAIAPDTAERIPALRDNFTLQGYRLANRNLELDF
                     RIKGPTLRPRSELASLPPPSITLVSGALETTSEVVQVFDLPRKILVDVFKIGAGIVGA
                     RKPPENNGH"
     gene            1742..2329
                     /locus_tag="PKW60_11710"
     CDS             1742..2329
                     /locus_tag="PKW60_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00144.1"
                     /translation="MEIKITKSARACSECERPFEHGEEIFSLIRLQDQAFVRNDYDKT
                     HWGPEKTQDAVAVWSTAYVDPKYEAQQPPEVFSPLRQAFYEAAESKDRAEIAKAYLAA
                     QLLRRQKVFRLVKEFDSDEGDVRLALFADRIGNRFIEVGDPNLSYQELETGRQALLHR
                     LRELEAAQAGEETGAETQTGEVKTDSTEMAHEAAG"
     gene            complement(2446..3420)
                     /locus_tag="PKW60_11715"
     CDS             complement(2446..3420)
                     /locus_tag="PKW60_11715"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPK00145.1"
                     /translation="MTDPEDDPLRARHMTSTIQNGDRSEVRSAAARRSICFALIVSCF
                     VLLTPLPALAGLPGREAQKDDMALQWYGDAEDIALLASLLSDADPHVREQAVCNLGQT
                     RNPDALAHVRKALDDTEPAVRRSAVLAAAQLRAPLSEQVVRQALASDDPETVLTAIAA
                     ARLQTWRELADALAKCLGHDQARVRVEAQWIFQKLPLEVNLQALGPGLTRDSLCLRRN
                     WRVYRSRWSAFPVERSRFPQVPGRGSTGRHRCRLARACAHLRRFAWWGAVRLYLSGRA
                     RPCGGWSAGLPVRARRDSHRFPGHPGLEAVVRCLVAPDWRSASVQIER"
BASE COUNT          732 a         1152 c         1087 g          610 t
ORIGIN      
        1 gcacggtttc gcagaaatcg tacggtctgc acgccggaaa tatctccggg cggctccacc
       61 tggaggaccg gtccgtgcag atccagcccc tcagctttgt cttccaggac aatgccgttg
      121 tcggcggatt ggcctacgac ttcgccagca aaacaggctc ggcatccatt aacggggcca
      181 ttgcaaaact ggaagacgtc catctcatcg ccatcaaaga tgtcgccgtg tcaggcggcg
      241 ccaccgtcaa ggcctcggcg gagttccgtc ccgaacggac ccgggccgaa tttctggcag
      301 aactgacgca ggcgcaggtc gattaccggt ggtttttcag aaagcctgcc ggcctgggcg
      361 tcaccctgca cggcacggcc gacttcaaac ctgggcagga gatcgcgctg aatggccagg
      421 ttaaagcagc agacacgttg cttgacgcgg agatgctctt ggcgcggtcc ggaggcaaat
      481 ggagccttag acgctcgctg accacggccg atacggcgga tatcgccacg attgcgaaat
      541 gccttcgcct gccctacaag cctcaaggca gtgccattcg ccgggtgtct tatgagtgga
      601 accgcgaccc ggaggaaccg accgcctggc gtgccttgct cgagggccat ctggatgaat
      661 tgagcctgct gcccgatggc ctcgagacgc cgcatgtgtt gaaggatgcc caactgcgcc
      721 tggcgttcac ccgaggcacg ccatccacgg gcgaactctg gcttcatgcc gccagcgccg
      781 ccacgccctc gttcggcgcc caatggttca atcccatcac ttgcccaccc gaactgattg
      841 accgcttccc tcccgtgccc cgtgactggg tatttcatct tgaagcggag catctgctcg
      901 caccgccctg ggaagcgagc gctttttcag gcgaagcgta caccacggcg gcccggatcg
      961 gtgtgaggca attccgggca cgcgtgggcg acggccgggt tgaaggcagc tacacactga
     1021 ccagggccga aaatgcctac tcaactacct tcgcctggga aaacataccg gccacgcccc
     1081 tgctgagcca tctcaagttc cccaacgttc ttgaaggctc cgtcgacggc aacatcacgt
     1141 actctatgga tcgcgacgat ccccacaccc tcgccggcga agggcgattc accgtgcggg
     1201 atggccgttt caacgccgac tatctcctgg cccagttcaa aggcccgctg caggaaagtg
     1261 caggcatcct ccccccttcg ctccagttca accgccttgc ctcaaacgta tcgttccaga
     1321 atgacgtcgt gacgaccaag gatgttgagc tgctcgcgga aggcatcaac gtgactggac
     1381 acgggaccta cgtgaccggc ggcgacatgg attacgccat cagactcgcc atcgccccgg
     1441 ataccgccga acggatcccg gcacttcgcg acaatttcac tctgcagggc taccggcttg
     1501 cgaaccggaa tctggaactg gattttcgta tcaaagggcc cacgctccgg ccccggagcg
     1561 agctggccag cctcccgccg ccaagcatca ccctggtcag cggcgccctc gagaccacca
     1621 gcgaagttgt gcaggttttc gatttaccta gaaaaatcct cgtcgatgtg tttaaaatcg
     1681 gggcaggtat cgtgggagcc cggaaaccac ccgaaaataa cggtcattaa cccaggacaa
     1741 catggaaatc aagatcacca aaagcgcgcg ggcctgttcc gaatgcgagc gcccctttga
     1801 gcatggggaa gagatcttct cgctcatccg gctgcaggat caggccttcg ttcgtaacga
     1861 ttacgataaa actcattggg gtccggaaaa aacgcaagac gccgtagcgg tatggtccac
     1921 cgcctacgtg gacccgaagt atgaagcaca gcagcccccc gaggtctttt cgccgctgcg
     1981 gcaggctttc tacgaggctg cggaatcgaa ggatcgcgcg gaaatcgcaa aagcgtatct
     2041 ggcagcgcaa ctgttgcgaa gacagaaagt cttccggctg gtcaaggaat tcgattctga
     2101 cgaaggggat gtccgcctcg ctttgtttgc agatcgcatc ggcaatcgtt tcattgaggt
     2161 tggcgacccc aacttgagct accaggagct cgaaactggg cggcaggccc tgttgcatcg
     2221 gttgcgggaa ctggaagcag cccaggccgg cgaagaaacc ggcgccgaga cccagacggg
     2281 cgaagtaaaa acggattcca cggagatggc acatgaggca gcagggtaag agacaatcac
     2341 gcggagaaga ccaggagcca gtcatccagc tcacccgcgg tcaaatggtg ggcgcggtct
     2401 gcgcgctgct ggttggcgcc cttgtattct acctgttggg cgtcgttacc tctcgatttg
     2461 aaccgatgct gaccgccaat caggcgcaac aaggcaacgc acaactgctt ccagccccgg
     2521 atgccccggg aaccgatgcg agtcgcgacg cgcccgcacg ggaaggccgg cagaccagcc
     2581 cccgcaggga cgcgctcgcc cggaaagata aagccgtacc gccccccacc acgcaaaccg
     2641 acgcagatgc gcgcaggccc gtgccagccg gcagcgatgc cgccccgttg agccgcgtcc
     2701 cggcacctgg ggaaaacgcg agcgctcaac cggaaatgcg gaccaccgtg accgatatac
     2761 ccgccagttc cgccgcagac aaagagagtc ccgcgtcaag ccggggccca aagcctgtaa
     2821 atttacctcc agcggcaact tctgaaaaat ccactgcgcc tccacccgca cccgcgcctg
     2881 gtcgtggccc aggcacttgg ccagggcgtc cgccagctcg cgccaggtct ggagacgggc
     2941 cgctgcgatg gccgtcagga ccgtttccgg atcgtccgaa gcgagagcct gacggacgac
     3001 ctgctcgctg agcggcgcgc gaagctgcgc ggcggccagg acagccgagc ggcgcacggc
     3061 cggctcggtg tcgtccaggg cctttcgcac gtgggccagg gcgtcggggt ttcgcgtctg
     3121 gcccaggttg cataccgcct gctcgcgcac gtgggggtcc gcatcgctca gcaatgaggc
     3181 caacagggct atgtcttccg cgtcgccgta ccactgcagg gccatgtcgt ccttctgggc
     3241 ctcgcgcccg ggcaacccgg cgagggcggg gaggggtgtc agcaagacga agcaggagac
     3301 gatcagcgcg aaacagatag accgccttgc ggcggcacta cgaacttcgc tgcgatcacc
     3361 gttctggatg gtcgaggtca tgtgtctggc tcgcaacgga tcgtcctcag gatcggtcat
     3421 cggttcgcct cagccggtgc acccgccaga ctgtggcggt gaacatggtc gcgatcgcga
     3481 tgcccgaaat catcaggtag ggctggacga tgtcataggt gaccatggtc gcgctgcccg
     3541 cggcggccat ggcggccgcg atcgggttga cgctgaagac g
//
LOCUS       DAOPBQ010000392         3575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21068_length_3575_cov_3.901136, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000392 DAOPBQ010000000
VERSION     DAOPBQ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21068_length_3575_cov_3.901136"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(464..1531)
                     /locus_tag="PKW60_11720"
     CDS             complement(464..1531)
                     /locus_tag="PKW60_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK00146.1"
                     /translation="MSSKKGFTLIELLVVIAIIGILAAILLPALARAREAARRSSCQN
                     NLKQWGLVFKMFANESKGEVWPYCSIDHGNTDTDVANKKMAAAVGAWQVYPEYVTDVM
                     IMACPSSGRYKDYAATDFSQGRNVLGGCDANFVTYATTHNEKDNPCYGKGAAPLSSTP
                     FGDNNKARFYNCDLDPNLCAPYWHTDMTKGKFIDVRSYRYFGRMIEQSWMTNTVDDYY
                     AAGHYIMGAGSYNTPDNSIRWANRNNEFSVTLPSGKPITIRRLREGIERFCITDINNP
                     AGAAQAQSQIVVMYDESRAYKAGGGGAEAERFNHVPGGMNILFMDGHVEWARYHGHDA
                     WPVNEFAYVYPAGITSGMDFP"
     gene            complement(2372..>3575)
                     /locus_tag="PKW60_11725"
     CDS             complement(2372..>3575)
                     /locus_tag="PKW60_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPK00147.1"
                     /translation="REARGCEVWDLDGRHYYDLSIHGIGASLLGYADPDVTRAVKRRV
                     ALGSWSTLNPPEEAALADRLFAIHPWANQARYTRTGGEAAAVAVRIARATTGRSHVAI
                     CGYHGWHDWYLAANLGEDDSLDGHLLPGLKPRGVPGELRGTAHTFRHGSREEFQAILD
                     QFGGRLAAVVLEPCRHHDPEPGFLEFVRDGAHRHGALLIFDEITIGWRLHFGGAHLRF
                     GVHPDMALFAKTLSNGHPMAAVIGTRDAMEGAHESFISSSYWTEGVGPVAALATLNKM
                     ERTRVWEHAERTGQRIKAIWASHAQAHGLPVTVDGGYPCLAHFAFDHPDATALRTYYT
                     QQMLERGFLAGTAVYATLAHSAAIVDRFEEAVDASFAALAAALAEGTLLRRLNGPLAH
                     SGFARLVK"
BASE COUNT          630 a         1078 c         1187 g          680 t
ORIGIN      
        1 ccccgctggc gggggtggcc gcgaagcggc cgggggtgga ctgcgtggat cctgcatggc
       61 cgcgttgaag aagggcaggc acaggggcct gcccctacac gctgcgaaaa ccgggatggt
      121 cccgggcagc ctcacgcagt tgccgttcgg attgcagggg cggccccctg tggccgccca
      181 tccggaaagc cccacacggc cgtctatcct tacgcgttga cccaaataca gggcaggcgc
      241 agccggcccc gcgtcttgtg tcccccgctg gcgggggtgg ccgcgaagcg gccgggggtg
      301 gactgcgtgg atcctgctgg ccgccttgaa gcagggcagg cacaggggcc tgcccctaca
      361 gggataacag aaagcgccgg gagcatcgct gctcccggcg cttcatctct acttcacgca
      421 gcccgcttac gcgaggctac gcactacacg ctgtgtcgtt ggctcacggg aagtccatgc
      481 cggaggtgat acccgccggg tacacatacg cgaactcgtt gaccggccag gcatcgtgcc
      541 cgtggtaacg cgcccattcc acgtggccgt ccatgaacag gatgttcatg ccgccgggga
      601 cgtggttgaa gcgttcggct tccgcgccgc cgccgccggc tttgtacgca cggctttcgt
      661 cgtacatcac gacgatttgg gactgggcct gggccgcgcc cgccgggttg ttgatgtcgg
      721 tgatgcagaa gcgctcgatg ccttcacgca ggcgacggat cgtgatcggc ttgcccgacg
      781 gcagcgtgac gctgaactcg ttgttgcggt tcgcccaccg tatgctgttg tccggcgtgt
      841 tataggagcc ggcgcccatg atgtagtggc ctgccgcata gtagtcgtcg accgtgttgg
      901 tcatccagct ctgctcgatc atgcggccga agtagcggta gctgcgcacg tcgatgaact
      961 tgcccttggt catgtcggtg tgccagtacg gagcgcacag attcggatcg aggtcgcagt
     1021 tgtaaaagcg cgccttgttg ttatcgccaa acggggtcga gctgaggggc gccgcgcctt
     1081 tgccgtagca ggggttgtct ttttcgttat gagtggtggc gtaagtaacg aaatttgcat
     1141 cgcagccgcc gagcacgttc cggccctggc tgaaatcggt cgccgcgtag tccttgtacc
     1201 ggccggagga cgggcaggcc atgatcatca cgtcggtgac gtattccgga tagacctgcc
     1261 aggcgccaac cgcggccgcc atctttttgt tggcgacatc ggtatctgtg ttgccgtgat
     1321 caatgctgca atagggccac acttcgcctt tggactcgtt ggcgaacatc ttgaagacca
     1381 gaccccactg tttgaggttg ttctgacagc tcgaacgccg tgccgcttcg cgggcccggg
     1441 ccaatgccgg aagaagaatc gcggccagga taccgatgat cgcgatgact accagcaatt
     1501 cgataagcgt aaagcctttt ttcgaactca tgataattga ctcctgtcat tcttgattat
     1561 cttctgccat ttaagcggtt ccggcgcctt accgtgtatg cgaagacgcc tccagggtac
     1621 agaacgttgt ctggcggccc tcctctctca tggctggcgg gcgcccccca ggcggcaagc
     1681 actatcttcc cgtccgctgg agccctgctg gattgcccgc acggggcaca gcctttcccg
     1741 tggagcctgg cgcttcgcgc ggcgccggag tcccggtggc gccgtgcgcg taactcgact
     1801 gcttcgcctg gaattcaggt ctccggtggc acctgaacct tggccacacc acagcgtaaa
     1861 caattccagg ctgccgtgag gtgaggcgac gcaacaggaa ggcaactgca catcacatgg
     1921 attagagcta ctttattcgc tcacctctgc atacccgaaa tctgaaacga ttattacact
     1981 ggtacgcgcg tttgtcaatc tcttttcttg ctcaaatttc aaggcgcgcg aggggccttc
     2041 taaacagcca tctctgttgt atttacataa tacgcacagt tacaggtcaa agaaatcggc
     2101 ttggagcaac gcgaaaaagg cttttttaat ccgaaaagcg cgagagaaaa acaagaaaaa
     2161 caccttagct gcttgccctg aaaactccga aaaaccctga actgctgatc tcgatatcca
     2221 cgcccatttt ctccccgatt tacgggactt ctgtgggtac agccatgatg gctgcggggc
     2281 gaaagcaggt ttttcaggca gtgcccgagg aggcggcgcg cccctgctca ggtgagggat
     2341 cccaagcgcg ggcatcggcc cgtgaacgtt ctcatttgac caggcgggcg aaaccgctgt
     2401 gggcgagggg gccgttgagg cgccgtaaaa gggttccttc agcaagggcg gcagccaggg
     2461 ccgcaaagga ggcgtcgacg gcctcttcga agcgatccac gatggccgcg ctgtgcgcga
     2521 gggtggcgta gacggcggtt ccggcgagaa acccgcgctc gagcatctgc tgggtgtaat
     2581 aggtgcgcaa cgccgtggcg tcgggatggt caaacgcgaa atgggccagg caggggtagc
     2641 cgccgtccac cgtgacggga aggccgtggg cctgcgcatg ggatgcccag atggccttga
     2701 tccgttgccc ggtacgttcg gcgtgttccc agacccgggt ccgctccatt ttattgaggg
     2761 tggccagcgc ggcgacgggt ccgacgcctt cagtccagta actgctgctg atgaacgact
     2821 cgtgcgcgcc ttccatggcg tcccgggtgc cgataaccgc ggccatggga tggccgttgc
     2881 tgagggtctt ggcgaaaagg gccatatcgg ggtgcacgcc gaagcgcagg tgggcgcccc
     2941 cgaaatggag ccgccagccg atggtgatct cgtcgaagat gagcagcgcg ccgtgccggt
     3001 gggcgccgtc gcgcacgaat tcgaggaagc ccggctccgg atcgtggtgg cggcagggct
     3061 ccaggacaac cgccgccagc cgtccgccga actgatccag gatggcctgg aactcctcgc
     3121 ggctgccgtg gcggaacgtg tgggcggtgc cgcgcagttc accgggcacg ccgcggggct
     3181 tgaggccggg cagcagatgg ccgtcgagcg aatcgtcttc gccgaggttg gcggcgaggt
     3241 accagtcgtg ccagccgtgg tagccacaga tggccacgtg gctgcgcccg gtggtggcgc
     3301 gggcgatgcg cacggccacc gcggccgcct cgcccccggt gcgcgtgtaa cgtgcctgat
     3361 tggcccaggg atggatggca aagagccggt cggcaagggc ggcctcttcg ggcgggttca
     3421 gggtggacca gcttcccagc gcgacgcgcc gcttcacggc gcgggtcacg tcggggtcgg
     3481 cataaccgag gagggaggcc ccgatgccgt ggatcgacag atcgtaatag tggcggccgt
     3541 caaggtccca gacctcgcag ccgcgggctt cgcgg
//
LOCUS       DAOPBQ010000393         3559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21160_length_3559_cov_3.502568, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000393 DAOPBQ010000000
VERSION     DAOPBQ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21160_length_3559_cov_3.502568"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1117
                     /locus_tag="PKW60_11730"
     CDS             101..1117
                     /locus_tag="PKW60_11730"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017319735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPK00148.1"
                     /translation="MMPLISPKEIKQALPMSDRAQETVMNGRKDVVRILSGEDKRMLA
                     VVGPCSIHDVDAALDYAQRLHQAHVKYGQRLFIVMRTYFEKPRTTTGWKGLVNDPDLD
                     GSYNLEKGLHIARRLLLDIAGMGLPAATEVLDPIVPQYIADLITWASIGARTTESQTH
                     REMASGLSMPIGFKNSTDGNLKVAINALLSARQSHHFLGIDQDGQTCVITTLGNPWGH
                     IILRGGGGSPNCDRVSVALAEEQLCAVGLKPRIMIDCSHDNSTRRYELQEKVLRDLMF
                     QRAEGTNSLIGMMLESNLNPGNQAIPADKSQLKYGVSITDPCMSWDMTDACLQYAYEH
                     LATP"
     gene            1284..2207
                     /locus_tag="PKW60_11735"
     CDS             1284..2207
                     /locus_tag="PKW60_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPK00149.1"
                     /translation="MLSRCMALIVVTCMSAGAQTDAFSPPGAGRSNVPLTIQGKDWHV
                     RIPANYEGDVELRYVFPPDRLSLQPSGEEVPFVAGDNGTVRFSVPPEKRTPPETVVVA
                     HWELERWEENIQTMEKDFPNIPENPILFVGSSSIRGWKVADYFPDLPVVNHGFGGSEY
                     FDALAYADRIIHPLRPRVVVLYDGDNDVARGKSPEWVAADMKAVVRTIHQANPQTPVV
                     ILSIKTSKARWELHEQMEQSNRLMAEFAESDPRVTFLDVNTPLLDAEGKPHDHYFLAD
                     HLHLSHEGYLVWTSLLRPVLDRLLAPAAPPE"
     gene            complement(2222..3490)
                     /locus_tag="PKW60_11740"
     CDS             complement(2222..3490)
                     /locus_tag="PKW60_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPK00150.1"
                     /translation="MSDLRDKLQQLLERQGVMRGAEWRRKTEELAQRRASGEFEIDRI
                     VPGEVVGDASEGFYLARTDFPLDTAHGNITLGEALLALPEHVALSANDTDLRDFTPET
                     AIFLDTETTGLAGGSGTVAFLVGAGYFDGTSFRLEQAFMRDFDDEEPMLRYLDRLFRG
                     RDTVVTYNGKSFDIPLLRARFIQNRMPFRLDSALQYDLLHAARRFWKRRLGDCGLTNI
                     EREVLAVRRHGDVPSAEIPELWLNYLRTRDARALQAVFYHHKMDILSLAALTARLSQS
                     LTLPQGLGFDHMEDRLSLVRLHFLQKHYREVMEHGQRLLEEEAESAIRCECLYLMGMA
                     SKRLKQWPQMEDAWTQLLAERPRDLVARLELAKHFEHRARNLLEAERLCAETVELFET
                     RGIPGSDEPLPGLEGFRARLERIQRKLSRG"
BASE COUNT          732 a         1145 c         1017 g          665 t
ORIGIN      
        1 tgcctgggcg caaaaacgcg tgaaacgaga taccagcctg atggaactcg atcggacaaa
       61 acgaatctta gagcatattc acgacgcgca cgtcgcggaa atgatgccgc tgatctcgcc
      121 aaaggagatc aagcaagccc tccccatgtc ggaccgcgca caggaaacgg tcatgaacgg
      181 ccgtaaagat gtcgtgcgca ttctgtccgg tgaggacaaa cgcatgctcg cggtcgtagg
      241 cccctgctcg atccatgacg tcgacgcggc gctggactac gcgcagcggc tgcaccaggc
      301 tcatgtgaag tacgggcagc gcctgttcat cgtcatgcgc acctattttg aaaaaccgcg
      361 caccaccacc ggatggaagg gtctggtcaa cgatccggat ctcgatggca gttacaacct
      421 cgaaaaaggc ctccatatcg cccggcgcct gctgctcgat atcgcgggca tgggcctgcc
      481 ggcagccacc gaagtcctcg accccatcgt gccgcagtac atcgcggatt tgatcacctg
      541 ggcttccatc ggtgcgcgta ccaccgagtc tcagacccat cgagagatgg caagcgggtt
      601 gtccatgccc attgggttca agaacagcac cgacggcaac ctcaaggtgg ccattaatgc
      661 cctcctttcc gcccgccagt cccaccattt cctcggcatc gaccaggatg gccagacctg
      721 cgtgatcacc accctcggca acccctgggg ccacatcatt ttgcgcggag gcgggggcag
      781 cccgaattgc gaccgtgtga gcgtggctct cgccgaagaa cagctgtgcg ccgtcggtct
      841 gaaaccgagg atcatgatcg actgcagcca cgacaacagc acccggcgct atgagctgca
      901 ggaaaaggtc cttcgcgacc tcatgtttca gcgggccgaa gggacgaact ccctgatagg
      961 catgatgctc gaaagcaacc tcaaccccgg taatcaggcc atcccagcgg acaagagcca
     1021 attgaaatac ggcgtgagca tcacggaccc gtgcatgagc tgggatatga cggatgcgtg
     1081 cctgcagtac gcgtacgagc acctcgccac gccctgacac gatcgtgacg gctcaggcag
     1141 cagcctccgg atcccccagc ctgcgcctgg ccgccccccc tgcccgcact gcccctcgtg
     1201 gtctcctcgc cgcccatgag aagcgtttga aaaatgtgtt atgctgccgc acacatgatc
     1261 gtcacctgaa aaggagaata tgaatgctgt cacgctgcat ggctttgatc gtggtcacat
     1321 gcatgtctgc gggcgcccaa accgatgcgt tttcacctcc cggtgccggc cgttccaacg
     1381 tccccctgac catccaggga aaagactggc acgtgcgcat cccggcaaat tatgaagggg
     1441 acgtcgagtt gcgctatgtg tttcctcccg atcgcctctc attgcagccg tccggggaag
     1501 aggttccgtt tgtcgccggc gacaacggga ccgtccggtt cagcgtccct ccggaaaagc
     1561 gcacgccacc cgagaccgtc gttgtggcgc actgggagct tgagagatgg gaagaaaaca
     1621 ttcagaccat ggaaaaagac ttccccaata tccccgaaaa ccccatcctg ttcgttggga
     1681 gttcgtctat ccgcggttgg aaagtcgcag actactttcc ggatctcccc gtggtaaatc
     1741 acggtttcgg gggttcggaa tacttcgatg cactggccta tgcggaccgg atcatccacc
     1801 cattgcgccc gcgtgttgtc gtcctgtatg acggcgacaa tgatgtcgcc cgcggaaaat
     1861 cacccgaatg ggtcgccgca gacatgaagg ccgtggtgcg cacgattcac caggcaaatc
     1921 cgcagactcc ggtggtcatt ctgtcgatta aaaccagcaa ggcccgatgg gaacttcacg
     1981 agcaaatgga acagagcaac cggctcatgg ccgagtttgc agaaagcgat ccgcgagtga
     2041 ccttcctcga cgtcaacacc cccctgctcg atgccgaggg caaaccccat gaccactatt
     2101 ttctcgcgga ccacctccat ctcagccacg agggctatct ggtctggacc agtcttctcc
     2161 ggcccgtcct ggaccgactg ctcgcccccg ctgcgcctcc cgaatgatcc ggcgggccag
     2221 tctatccccg gctgagcttt cgctggatgc gttcgagccg cgcgcggaac ccctccaggc
     2281 ccggcagggg ctcgtcggag cccgggatgc cgcgcgtttc aaaaagttcg acggtttccg
     2341 cacacaggcg ttcggcctcg aggagattgc gtgcccggtg ctcaaaatgt ttggccagct
     2401 ccaggcgcgc aaccaggtcg cgcggccgtt ccgcgagcag ctgggtccac gcatcctcca
     2461 tctgaggcca ttgctttaac cgtttgctgg ccattcccat gagatacagg cattcgcagc
     2521 ggatggccga ttccgcctct tcttccagca ggcgctgacc gtgttccatc acctcgcggt
     2581 agtgcttctg caggaaatgc agtcgcacca gcgagaggcg atcctccatg tgatcgaatc
     2641 ccaggccctg cggcaaagtg agactctgtg acaggcgggc cgtcagagcg gccagcgaga
     2701 gtatgtccat tttgtggtga tagaaaaccg cctgaagcgc ccgggcgtcg cgcgtgcgca
     2761 ggtaattcag ccagagttcc gggatttcag cgctgggaac gtctccgtgc cgccgtacgg
     2821 ccaatacttc ccgctcgata ttcgtcagcc cgcaatcccc aagccggcgt ttccagaaac
     2881 ggcgggccgc atggaggaga tcgtactgga gcgccgaatc cagccgaaaa ggcatccggt
     2941 tctgtatgaa ccgggcccgc agcaagggta tgtcaaagct cttgccgttg taggtgacga
     3001 ccgtgtcgcg cccgcgaaac agtctgtcaa gataccgcag catcggctct tcgtcgtcaa
     3061 aatcgcgcat gaaggcctgt tccaggcgga acgatgtgcc atcgaaataa ccagcgccca
     3121 ctagaaaggc gaccgtcccc gacccgccgg ccaatccggt ggtctccgta tccagaaaga
     3181 tcgctgtctc gggcgtgaag tcccgcagat cggtgtcgtt cgctgacaag gccacgtgct
     3241 cgggaagtgc gagcagagcc tcgcccagcg tgatgtttcc gtgcgcggtg tccagcggga
     3301 agtcggtgcg ggccagataa aagccctcgc tggcatcccc caccacttcg ccggggacga
     3361 tgcggtcgat ctcaaactcg cctgacgccc ggcgctgagc aagctcctcg gttttccggc
     3421 gccactcggc gccgcgcatc acgccttgtc tttcaagcaa ttgctggagt ttatcacgca
     3481 aatccgacat gacttattcc ggtgcggcgc ccgcctgaac ctgcggatcg ggcaatccca
     3541 aagctatccg ggcgagctt
//
LOCUS       DAOPBQ010000394         3554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21198_length_3554_cov_3.822235, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000394 DAOPBQ010000000
VERSION     DAOPBQ010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21198_length_3554_cov_3.822235"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKW60_11745"
     CDS             complement(<1..376)
                     /locus_tag="PKW60_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HPK00151.1"
                     /translation="MIRAMFTSATGMMAQQLHVDTIAHNLANVNTTGFKKSRVNFQDL
                     LYETLKPAGSETTTGATIPEGIQVGHGVRPASIAKLFTQGNMIQTGNELDLVIEGDGF
                     FQIELPDGTIAYTRDGNFKLNND"
     gene            complement(394..1152)
                     /locus_tag="PKW60_11750"
     CDS             complement(394..1152)
                     /locus_tag="PKW60_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HPK00152.1"
                     /translation="MIQGLYAAASGMLGMERRQDVIANNIANAATPGFRRQVAVSEGF
                     NEILLRSMRHPFRLDAERAPGGGQKLDETFTDIQAGNVIVTEDPLNIALQGPGFIAVD
                     TPHGERFTRNGKLAVDANGLLTTADGFHVQSLAGGGIDVSGGGITIDQEGNVYSQGLF
                     VAQIRLLEFEDPHMLTRTGANLYAASDEAMRRSTETTDTSVLHKSLELSNVQVPAEMV
                     QMMLGARVYAANQKVINAIDESVASLISEVAKPA"
     gene            complement(1484..3076)
                     /locus_tag="PKW60_11755"
     CDS             complement(1484..3076)
                     /locus_tag="PKW60_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPK00153.1"
                     /translation="MNLRGFIVVMGLCAVLAGMPPCADAESHPVQAGAEAAPVQAANV
                     QKRPPDPLRGVYLNSASFYERKITETLHYASEAGLNAVVLHVKDPYGHLYWEPQNTLA
                     REINASKGDKQLRAAIQRFADAGLWIIAKQDLFQDTLLATQRPDWAVQDSATGKPWLN
                     RQKLAWGNPHDVRVWDYNIALAKELLALGFDEIQFDYVRFPSDGDLSRVHYPKVLNGK
                     DRLQVIGAFLEKANKELKPLGATISVDLFGFVAWKTDDFGVGQRIEEIAPHVDAICPM
                     LYPSHFPKNFMGKDDPAKYPREIMEESIKRLQKRTQVDVRAWVQGFWYKPSEITEQIK
                     GIEATGCKSYFIWSPSSRYEETYAALAAYQNKTFAQPKYYPTLAELAEKGRSDLGGVS
                     RTVNATDYVKGATILCLDQSTAERKSTYTTPRQLVDTLDEAILDAILQSRGVESSWKV
                     PRGTKLDRVVGMLLNDLGISARAMRAEPIRISWKGDCRFYRDAGGRASSAPVSDPAPA
                     PSAQDAAALESAAQKEDTTPTQ"
     gene            3344..>3554
                     /locus_tag="PKW60_11760"
     CDS             3344..>3554
                     /locus_tag="PKW60_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPK00154.1"
                     /translation="MLDKKTIRQQKLAARRALTPAEAAEKSAAICAAAAALPEFERAS
                     AVLTYVASKDNEVDTQPLILRLLASS"
BASE COUNT          672 a         1030 c         1104 g          748 t
ORIGIN      
        1 catcgttgtt gagtttgaag ttgccatcgc gggtatacgc gatggttccg tccggcagct
       61 cgatctggaa gaatccgtcg ccttcaatga ccaggtcgag ttcatttccg gtttggatca
      121 tattgccctg ggtaaacagt ttcgcgatgg atgccggccg gacgccatgc cccacctgga
      181 tgccctcggg gatcgtcgcg ccggtggtcg tttcggaacc ggccggcttc agggtttcgt
      241 acaacaggtc ctggaaattg acccggctct tcttgaaccc cgtcgtattc acgttggcca
      301 ggttgtgcgc gatggtgtca acgtgcagct gttgcgccat catgcccgtt gccgaagtga
      361 acattgcacg aatcatatgg aatcctccgg gccctaggcg ggcttggcca cttcactgat
      421 cagactggcg accgattcgt cgatggcatt aatgaccttc tgattggcgg cgtagacgcg
      481 tgcgccgagc atcatctgga ccatctccgc cggcacctga acattggaca gctcgagcga
      541 tttgtggagt acgctcgtgt ctgtcgtctc ggtggatcgg cgcatggctt cgtccgaggc
      601 ggcgtagagg ttggcgccgg ttcgtgtcag catgtgggga tcttcaaatt ccaggagacg
      661 gatctgggcc acaaacaggc cctggctgta gacattgcct tcctgatcga tggtgatgcc
      721 gccgccgctg acgtcgatcc cgccgcccgc caggctctgc acatgaaatc catcggcggt
      781 agtcagaagg ccattggcat ccaccgcaag tttgccgtta cgcgtgaagc gttcgccgtg
      841 aggcgtgtcg accgcgatga acccgggacc ctgcagggcg atgttgagcg gatcttcggt
      901 tacgatgaca ttgcccgcct ggatatccgt gaatgtctcg tcgagcttct ggccgccgcc
      961 gggagcgcgt tccgcgtcga ggcggaaggg gtggcgcatg ctgcgcagaa ggatttcatt
     1021 gaatccttcg ctaacggcta cctggcggcg aaatcccggc gtggccgcgt tggcgatgtt
     1081 gttggcgatc acgtcctggc gccgctccat gcccagcatg cctgaggccg ccgcatacaa
     1141 gccctgaatc atcgtacatc tcccccacgc tgccgtgggc tccccattca tcccgcaggc
     1201 gtgcaccacg ggatggcctg gcgccggcgt ttggcaacaa ctgggtatca ggaacgcttt
     1261 tgggcaccgc tcccaactgc caggccatcc gcgataccgg gtacacagca gcgcttcttg
     1321 ccgctgactc aggagacgca aagtgtatgc caagcgcgcc gatgagacgc gcatgcctta
     1381 atgcacttgt ttgaaaggac ttacagactg ggcgccagca gggcgtgctg cgcaatgcca
     1441 gtaaaacttg ccgggaggcg gaacattctg caggggggcg cggtcactgc gtgggtgtcg
     1501 tatcttcttt ttgcgcggct gattccaacg ctgccgcatc ctgagcagag ggcgccggcg
     1561 ccggatccga cacgggtgca gatgaggcac gcccgccggc atcgcggtaa aaccggcaat
     1621 cgcctttcca ggagatccgg atcggttcgg cgcgcatcgc acgcgcggag atgccgagat
     1681 cgtttaacag catgccaacc acacggtcga gcttggtgcc gcggggtact ttccaggagg
     1741 actcgacgcc gcgggactga aggatggcgt cgagaatggc ttcgtcgagg gtgtccacga
     1801 gctggcgggg cgtggtgtat gtgctctttc gctccgcggt ggactggtcc aggcacaaaa
     1861 tggttgcgcc tttcacgtaa tctgttgcgt tgaccgtgcg ggacacgccc ccgagatccg
     1921 accggccctt ttcagcgagt tcggcgaggg tcgggtagta cttgggctgt gcgaaggttt
     1981 tgttttgata cgcggcgagt gcggcgtagg tctcttcata acgcgacgaa gggctccaga
     2041 tgaaatagct tttgcagccc gtcgcctcaa tgcccttgat ctgctcggtg atctcactgg
     2101 gtttgtacca gaacccctgg acccaggcac ggacatcgac ctgggtgcgc ttctgaaggc
     2161 gttttatgct ttcttccatg atctcccgtg gatatttcgc gggatcgtct ttgcccatga
     2221 aattcttcgg gaaatgcgag ggatacagca tcgggcagat ggcatcgaca tgcggggcga
     2281 tttcttcaat gcgctgcccc acgccgaaat cgtcggtctt ccacgccaca aacccgaaaa
     2341 gatccacgga aatggtggcg cccaggggtt tcaattcctt gttggctttt tcaaggaaag
     2401 caccaatgac ctgcaggcga tctttgccat tcaatacctt gggatagtgc acgcgggaca
     2461 aatcgccatc ggaaggaaaa cggacgtaat cgaactggat ctcatcgaag ccgagcgcaa
     2521 gaagctcttt ggcgagggcg atattgtaat cccagacgcg cacgtcgtga ggattccccc
     2581 aggccagttt ctggcggttg agccagggtt ttcccgtggc gctgtcctgg acggcccagt
     2641 cggggcgctg ggttgcgagg agcgtatcct gaaacaggtc ctgcttggcg ataatccaca
     2701 atccagcgtc agcaaagcgc tggatcgcgg cgcgcagctg tttgtcgccc tttgaggcgt
     2761 tgatttcgcg ggcaagagtg ttttgcggtt cccaataaag gtgcccgtag ggatctttga
     2821 cgtgcaacac gaccgcattg aggccggctt cactcgcgta atggagcgtc tccgtaattt
     2881 tgcgctcgta gaagctggcg gaattcaggt agacgccgcg cagagggtcc ggcgggcgtt
     2941 tctgcacgtt ggccgcctgc actggggcag cctcggcgcc cgcctgaaca ggatgcgact
     3001 cggcatcagc gcatggcggc atgcccgcca gtacggcaca caatcccatc accacgataa
     3061 acccgcgtag attcattcaa attggtccat ttctatccgc caccgcttat agttccgcac
     3121 gaaaaaggtg ctgtctttac ttcagttgaa ccgtccgcga agaaacaccg cgccccaggg
     3181 acgtggcggg atcattgcgg ttcgggaacg attccctggg cctggattcg tgcctggcgg
     3241 gcggcattat accacgaggg cggcgcgtca accgaaaccg cttgactgat cccgccatgc
     3301 cttgtataag agcggaaaac ggaggcgcaa ccgaaggcac ggcatgctcg acaagaaaac
     3361 aattcggcag cagaagctcg cggcgcgaag ggcgcttacc ccggctgagg ctgcagagaa
     3421 aagcgcggca atttgcgcgg cggccgcggc attgccggaa ttcgagcggg catccgccgt
     3481 gctgacctac gtggcctcga aagacaacga ggtcgacacc cagcccctga ttctgcgcct
     3541 actggccagt tcgc
//
LOCUS       DAOPBQ010000395         3552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21220_length_3552_cov_3.233915, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000395 DAOPBQ010000000
VERSION     DAOPBQ010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21220_length_3552_cov_3.233915"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKW60_11765"
     CDS             <1..272
                     /locus_tag="PKW60_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPK00155.1"
                     /translation="LLGLLGTVAGMIKAFAQIQALEGLVSPSDLAEGIGNALVTTAAG
                     LTVAIPTLVAYNYFVSRVENMILEMEISSSELIELLTRHRGEREI"
     gene            345..761
                     /locus_tag="PKW60_11770"
     CDS             345..761
                     /locus_tag="PKW60_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPK00156.1"
                     /translation="MKAQLDLTPLIDVVFQLILFFMLSSTFVVQSTVNIQVPEALGAT
                     ALEKKDLTVTLAYGEGGPDGHGPIYVDNVEVASMGQLSQKLADEKRARPDLMLLIRSD
                     GRTETARLVKVLGIARSLGITNFGIAAQPPSESQEG"
     gene            772..1611
                     /locus_tag="PKW60_11775"
     CDS             772..1611
                     /locus_tag="PKW60_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00157.1"
                     /translation="MLRNRLFLAALSVSALFHLSMVTLFSIVFEFPVKRIDYYSFRIL
                     SEEPALSRSAAASPGERMAGLPTLQNPLRDTGLESLGLEPAPSDWAAGLPAIELPRLE
                     FAEMQMLEAREEGLSIRSRLVDFLDAPLSEPWSRLSRGLERLGDALSSLGWFDDADKE
                     ALEKPAPKLVARPAEGFEAYVEWMSEPLNRELLFSPPIEALFRLHPEDLREPITLVFR
                     VNPRGEVVEVLTPLPDEAGVVVSAARALTSYRFEPLDAPDARDQHGTLLITAARASEG
                     ALP"
     gene            1608..1970
                     /locus_tag="PKW60_11780"
     CDS             1608..1970
                     /locus_tag="PKW60_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00158.1"
                     /translation="MIQLEIGQALILYSGVLFVIAAGIWLYTEISAWRSHRTLGKQYL
                     WRCIFCGFSYLDEEADTLSKCPRCESLNSASDKHARVVQVRGKTVEAPEPAGTTPRRN
                     TSKRKHPTQRRRGPRKRR"
     gene            complement(1997..2584)
                     /locus_tag="PKW60_11785"
     CDS             complement(1997..2584)
                     /locus_tag="PKW60_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00159.1"
                     /translation="MPRKISILLQHGSLAALIALLCASLADAAQMDASAGPDRSQRLG
                     VQSGYQRPHSQHYSQGTSEGVHFRTDVPTYQHRTNSTVARVGPRGAYIATGQGVAISH
                     SGRYYHRPYRNYAYSCPVRSYWVPGHWETVHKNVWIPGYWTNRLIPAVYRREVVNGRE
                     ILVLVQEERWENVWVEGRYETRPESVWVAGYWARY"
     gene            2826..>3552
                     /locus_tag="PKW60_11790"
     CDS             2826..>3552
                     /locus_tag="PKW60_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00160.1"
                     /translation="MLGALEAGNSPRRYHACLSRLACEANPDLLHVVRDAGVTDIWQA
                     AFFYGYWYDTVEGLEKGRNQVESLGLRWHPLNVPLGHPGDSLGDASGQTALTPPAHWR
                     MAERVDGKAHSGTSLHEPATAENVAAVREIAALNPDILFLDDDFRLAVGPGVIGGCFC
                     AEHRERFLQTGGYSESAWEELRDDARARRLSPLVRSWVEFTCDELTGCFQAQQDAAPG
                     VRLGNMVMYLGAEKAGIRLTDYRD"
BASE COUNT          676 a          970 c         1186 g          720 t
ORIGIN      
        1 ctctccttgg gcttctcggt acggtggcgg gtatgatcaa ggcgtttgcg cagattcagg
       61 cgctggaagg actggtgagc ccgtcggacc tggcggaagg tatcggaaac gcgctggtca
      121 cgacggcggc cggattgacg gtcgcgatcc cgacgctggt ggcctacaac tatttcgtgt
      181 cccgggtcga aaatatgatt ctcgagatgg agatcagttc ttcggaactg atcgagctgt
      241 tgacacggca tcgcggtgaa cgggagatct aggggaagaa attttaacac tgggtgtatg
      301 aagcgatgca attcagagat ggcgacggta atagcaagag gaaggtgaag gcccagctgg
      361 acctgactcc attgatcgac gtggtgttcc agcttattct gtttttcatg ctctcgtcga
      421 cgtttgtggt tcagagcacc gtaaacattc aggttcctga ggcgctcggg gcaaccgcac
      481 tcgagaaaaa agacctcacg gtcactctgg cgtacggcga aggcggcccg gacggccatg
      541 gcccgatcta cgtcgacaac gtcgaagtgg cgagcatggg acagctgtcg cagaagctgg
      601 cggacgagaa gcgggcgcgt ccggacctga tgctgctgat ccgctcggac gggcgcaccg
      661 agaccgcgcg actggtgaag gtattgggca ttgcacggag cctcggaatc actaatttcg
      721 gtatcgcggc gcaacctcct tcagaatctc aggaagggta acaaaggagg catgctcagg
      781 aaccgtttgt ttctggcagc cctttctgta tccgcactgt tccatctgtc gatggtcacg
      841 cttttctcga tcgtctttga atttcccgtg aagcgcatcg actactacag ttttcgtatc
      901 ctgtccgagg agccggcgct atcgaggagc gcggcggcga gtcccggaga gcgcatggca
      961 ggcctgccca cgctgcagaa tccactgcgc gacaccggac tcgaaagcct gggactggag
     1021 cctgcgccgt cggactgggc ggcgggactt cctgcaattg agctgccccg gcttgagttt
     1081 gccgagatgc agatgcttga agcgcgcgag gaagggctct ccatccggtc gcgcctggtg
     1141 gatttcctgg acgcgccatt gagcgagccg tggtcgcggc tgagccgcgg gctggaacgc
     1201 ctgggggacg cgctctcatc gctgggttgg tttgatgacg ccgacaagga ggcactggag
     1261 aagccggcgc caaagctggt ggcgcggcct gccgagggat tcgaggcgta tgtggaatgg
     1321 atgtcggaac cgctgaaccg cgaactgctg ttttcaccgc cgatcgaggc cctgttccgg
     1381 ttgcatccag aggatctgcg cgaacccatc acgctggtgt ttcgtgtaaa tccccggggg
     1441 gaagtggttg aagtgctcac gccgctgccc gacgaggcgg gggtcgtggt gagcgccgca
     1501 cgcgcattga cctcgtaccg tttcgagccg ctggatgcac cggacgcccg ggatcagcac
     1561 ggcacgcttc tcatcaccgc cgcgcgggcc agtgaggggg ctttgccatg atccagctgg
     1621 agataggcca ggcgctcatc ttatatagcg gcgtgctgtt tgtgatagcg gccgggatct
     1681 ggctgtacac ggaaatcagc gcgtggcgct cgcaccgcac cctcggaaaa cagtatctgt
     1741 ggcgctgtat cttctgtgga ttcagttact tggacgagga ggcggacacc ctgtcgaagt
     1801 gtccccgttg cgaaagtctc aattcggctt cggacaaaca cgcgcgcgtg gtgcaagtgc
     1861 gtggtaaaac cgtcgaggcg ccggaaccgg ctggcacgac gccgcggcgg aatacctcga
     1921 aacgaaaaca tccaacccag cgccgacgcg gcccgcgcaa gcgccgctga ccgcgctgtt
     1981 ccgcagtcag cggggctcag taccgggccc agtaaccggc aacccaaacg ctttcgggtc
     2041 gggtctcgta gcggccttcc acccagacgt tttcccagcg ctcctcctga accaggacca
     2101 ggatctcccg gccattgaca acctctcttc ggtagacggc ggggatcagg cggtttgtcc
     2161 aatagccggg aatccagaca tttttatgga cggtttccca atgtccgggc acccagtacg
     2221 agcgaacagg gcaggagtag gcgtaattcc ggtaggggcg gtgataatac cgtccggaat
     2281 gggagatggc cactccctgg ccggtggcaa tgtacgcgcc gcgtggaccg acccgggcga
     2341 cggtgctgtt cgtccgatgt tggtaagtgg gcacatcggt acggaaatgg acgccttccg
     2401 aggtgccctg gctgtaatgc tgggagtgcg gccgctggta gcccgactga acgccgagcc
     2461 tctggctccg gtcagggccg gccgaggcgt ccatctgagc ggcatcagcc aggcttgcac
     2521 acagaagcgc aatcagggcc gccagtgacc cgtgctggag tagtattgaa atttttcgag
     2581 gcattgcagg tgctccagtt cacttttcgc ctatattgta tactatacct tcatagaata
     2641 acgacgacag aaagccgggt taattcacgg cacgtggccg ccggcgctgt tggagcgggc
     2701 agaaatatgc ttcgggctgt gcatcccgtc tggcggggca tcctgacatg cgaaaggggt
     2761 ttggaatgta cctgaatcgg cgtaattttc taaagtgcgg tgctgcaata ggttgtggga
     2821 tacctttgct gggtgcgctg gaggcgggta acagcccgag gcggtaccac gcctgcctgt
     2881 cccggctggc ctgcgaggca aacccagatc tcctgcatgt ggttcgtgat gctggagtta
     2941 cggacatatg gcaggccgcg ttcttctatg ggtactggta tgacaccgtc gaggggttgg
     3001 agaaaggccg taatcaggtg gaatcattgg ggttacggtg gcatccgctg aatgttcctc
     3061 tggggcatcc gggcgattcg ctgggagatg catcgggcca gacagcgctt acgccccccg
     3121 ctcattggcg gatggccgaa cgtgttgacg gtaaagcaca tagcggaacg tcactgcacg
     3181 aacccgcgac agctgagaat gtggcggcgg tccgggagat tgcggcgttg aatccggata
     3241 tcttgttttt ggacgacgac ttccggctgg cggtggggcc gggggtgatt gggggctgtt
     3301 tctgtgcgga acaccgcgaa cggtttctcc agacgggcgg atattccgaa tccgcctggg
     3361 aagaactgcg cgatgacgcg cgtgcgaggc gactctctcc gttagtgcgg tcctgggtag
     3421 agtttacctg cgatgagctg acgggctgct ttcaggcgca gcaggacgcc gcgcccggtg
     3481 ttcgtctcgg gaatatggtc atgtacctcg gggccgagaa agcggggata cgcctgacgg
     3541 actaccggga tg
//
LOCUS       DAOPBQ010000396         3541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21299_length_3541_cov_3.968445, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000396 DAOPBQ010000000
VERSION     DAOPBQ010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21299_length_3541_cov_3.968445"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PKW60_11795"
     CDS             <1..542
                     /locus_tag="PKW60_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00161.1"
                     /translation="FLWDDAKEVYNVSSSSFLFKGADGKDKIMTLEVRPWYTNDEMGG
                     TSFGVMFYGSEGVITFPSYSGYLAYKCKKFGESELQPAQKRMDGGDKNHYQNFIDCVI
                     SRDASQITAPPIEGHYSSALSHYALTGARINRVLEINDQTGDITNVDWTAIPGLTEEE
                     ATLYLKRDYRDGFEIRDEV"
     gene            758..2194
                     /gene="cls"
                     /locus_tag="PKW60_11800"
     CDS             758..2194
                     /gene="cls"
                     /locus_tag="PKW60_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPK00162.1"
                     /translation="MGTLLNIIALVHEVGNWIIRVSMLLAITRRHRPQSAMVWLLVVF
                     FSPWFGLVLYAFLGSNRLPRQRVDRHKRFLARIDALRSRIIGSPGLTQPQLEPAFAST
                     VRLVQRLGHMPVLGGNHAEIICDNDAFLDRIIADIDRAAHHVHALYYIFGDDAAGRRF
                     ADALIRAERRGVACRLLVDAVGSWRMLARMAPELARAGVEVFEALPVGFFRRGMARWD
                     LRNHRKLVVIDGQIAFSGSHNLIEPSYGTRDFIWHDLSVRLTGPVVSQLQAVFISDWY
                     FENNDLLEDPAFFPENPIQGDIAAQVIPSGPNYPTENYQRLLVSAIHGAQKHVTITTP
                     YFVPDESLLNALETAALRGAEINLIVPQVADQRLVGAATQAYYEELLTWGINLYLYQP
                     ALVHAKTMAIDDAVAFVGTSNFDIRSFALNFELNMVFYSAALAGELRKRHEGYMKNST
                     RLELGRWEARPASSRIFQNVAKLFSPLL"
     gene            2431..>3541
                     /locus_tag="PKW60_11805"
     CDS             2431..>3541
                     /locus_tag="PKW60_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPK00163.1"
                     /translation="MSARVFLVGSEAHTARAVTAARQAELEPVVYRDRGDGPEALAGA
                     ARGARVDGIYSADEAHAELVAAAAQSLGLPCFSTKAARLLHNKEALRAVLGAHTQLNP
                     RYGVANTLAGVRHALQQLGLPAVVKPVDGAGGSGALIVRDIDDAPLAFARASRAASNG
                     RVLVEPHLAGTEYRVVCHCGDSGWGTLTVLSNVPAGRDHLYDRALVAPVRVAGPLRAR
                     LREQVRAVIRLIGRFEGVVVFEFLSVDDDLYLLEMAHINETPALATGLFPAICGIDLL
                     ELDVASSIGRAFASKPRFTVCGALWWLEPRSGVVASVHGISEARSVPGVAAVEIAAKV
                     GTVLSHQVDAPSRDAAGYVLATARTPRRALEKAQYA"
BASE COUNT          663 a         1091 c         1099 g          688 t
ORIGIN      
        1 tgttcctgtg ggatgacgcc aaggaagtct acaacgtctc ctcgtcgtcg ttccttttta
       61 agggcgccga cggcaaagac aagatcatga cgctcgaagt gcgcccgtgg tacacgaacg
      121 atgaaatggg cggcacctca tttggcgtga tgttctacgg ctcggaaggc gttatcacct
      181 tcccgagcta ttccggatac ctcgcctaca aatgcaagaa gttcggcgag agcgaattgc
      241 agccggcgca aaaacgcatg gatggcggcg acaaaaacca ctaccagaat ttcattgact
      301 gcgtgatcag ccgcgacgcc agccagatca cagcaccccc catcgaaggg cattactcgt
      361 ccgcgttgtc gcactacgcg ctcacgggcg cgcgcatcaa ccgcgtcctc gagatcaacg
      421 accaaaccgg ggatatcacc aacgtcgact ggactgctat cccgggactg accgaagaag
      481 aagccaccct gtacctcaaa cgcgactatc gtgacggatt cgaaatccgc gacgaagtct
      541 gagcgcttca ggatgaaaac gtaccacgcc cggcgcggct ccagcccgcc gggcgtgcct
      601 ttttttgtcc aggttctccg ccggccgcaa tcgcaatcgt accgtgcgaa cccggcaatc
      661 tgcagcggat cttccagccc ggcttgcttt aggcatccgg ttatccaata gaatgaggcg
      721 aggagcatac tgaaaccggc ggtgagggga ctcggctgtg ggaacccttc tcaacataat
      781 cgcgcttgtc cacgaggtgg gcaactggat catccgcgtc agcatgctgt tggcaattac
      841 acgccggcac cgcccgcagt cggccatggt gtggctgctg gtggtctttt tctcgccctg
      901 gttcgggctg gtgctgtatg cctttctggg ctcgaaccgg ctgccgcgcc agcgcgtcga
      961 ccggcacaag cggtttctgg cccgcattga cgccttacgc agccgtatca tcgggagccc
     1021 gggcctcacg caaccgcagc tcgagcccgc cttcgcctcc acagtgcgcc tggtgcagcg
     1081 gctgggccac atgcccgtgc tcggcggcaa tcacgctgag atcatctgcg acaatgacgc
     1141 gtttctcgat cgcatcatcg ccgacatcga tcgcgccgcg catcacgtac acgcgctgta
     1201 ttacattttc ggagacgatg ccgccggacg gcgcttcgcg gatgccctca ttcgcgccga
     1261 acggcgcggt gtcgcctgcc gcctgcttgt cgatgccgtg ggttcctggc ggatgctcgc
     1321 gcgcatggcg ccggaactgg ctcgggcagg ggtggaggtg ttcgaggcgc tgcccgtagg
     1381 cttctttcgc cggggcatgg cgcgctggga tctgcgaaac caccgcaaac tcgtggtgat
     1441 cgacggccag atcgcgtttt cgggctcgca caacctgatc gaacccagtt atggcacccg
     1501 cgacttcatc tggcatgacc tgtctgtgcg cttgaccggc cccgtggtgt cgcaattgca
     1561 ggcggtattc atctcggact ggtattttga aaacaatgac ctcctggaag accccgcttt
     1621 ctttccagaa aacccgatcc agggagacat cgcggctcag gtgattccca gcggccccaa
     1681 ctaccccacc gaaaactacc agcggctgct tgtatcggca attcacgggg cacaaaagca
     1741 tgtgaccatc accacgccgt atttcgtgcc cgacgaaagc ctgctcaacg cgctcgaaac
     1801 cgcggcgctc cgtggagccg agattaacct tatcgtgccc caggtggccg accagcgtct
     1861 cgtcggcgcc gccacgcaag cctactatga agaactgtta acctggggca tcaacctgta
     1921 cctgtatcag ccggccctcg tacatgccaa aaccatggcc atcgacgatg ccgtggcttt
     1981 cgtcgggacc agtaatttcg atatccgctc gtttgccctc aatttcgagc tcaacatggt
     2041 gttctattcg gcagcgctgg ccggggagtt gcggaaacgg cacgaaggct atatgaaaaa
     2101 ctccacgcgc ctcgaattgg gccggtggga ggctcgcccg gcatccagcc gtatcttcca
     2161 gaacgttgcc aaactgttca gtccgcttct gtagcctgta tcccccggag cacgggctca
     2221 accggcctga atcgaaagcc atggccagcc cggtacccgt ttgcagctcg tgggacggaa
     2281 acggtatcgt tatttcgctg aatgcagccg ggggatgttt tttcgcggtt tgaacgtctg
     2341 caaagggccg gatagggccc gtttttgaaa atgagccctg ctgacgcagg cgagcaacat
     2401 gcagcgcctg cccggcaagt gagaccacga gtgagcgcac gtgtctttct agttgggagc
     2461 gaagcccaca ccgcccgggc ggtaacagcc gcccggcagg ctgaactgga gcctgtggtg
     2521 tatcgcgatc ggggcgatgg gccggaagcc ctggccggcg ccgcccgcgg tgcgcgtgtg
     2581 gatggtattt acagcgcgga cgaagcacat gcagaactgg tcgcggccgc tgcccaatcg
     2641 ctgggcctcc cctgcttctc gaccaaagcg gcacgtttgc tgcacaacaa ggaagcgttg
     2701 cgcgccgttc tgggagcgca cacccagctg aatccccgct atggcgttgc aaacacactg
     2761 gccggggtgc ggcacgcctt gcaacagctg ggactgcccg cggtggtaaa gcccgtggat
     2821 ggtgcgggcg gaagcggggc cctgattgtg cgggatatcg acgatgcacc cctggcgttt
     2881 gcccgggctt cgcgtgcggc ctccaatggc cgggtactgg tggaaccgca cctggcggga
     2941 acagagtacc gcgtagtgtg ccattgcgga gacagcggct ggggaacgct cacggtgttg
     3001 agcaacgtgc ccgcggggcg cgaccatctg tacgaccggg ccctggtcgc cccggtgcgc
     3061 gtggcaggtc cgctgcgcgc gagactgcgg gagcaggttc gtgcggtcat acgactcatc
     3121 ggaagatttg aaggcgtcgt ggtatttgaa tttctgtcgg tagacgatga cctgtatctg
     3181 ctcgaaatgg cgcatatcaa tgagacaccc gcactggcga cgggattgtt tccggccatc
     3241 tgcggcatcg acctgctgga actggatgtg gcgtcgagta tcggtcgcgc ttttgcatcg
     3301 aaaccccggt ttacggtgtg tggggcgctg tggtggctgg aaccgcgcag cggggtggtg
     3361 gcgtccgtgc atgggatttc agaagcccgg tcggtaccgg gcgtggccgc ggtagagatc
     3421 gcggcgaaag ttgggacggt gctcagccat caggtggacg ccccttcacg cgatgccgcc
     3481 ggctatgtgc tggccaccgc gcgcacaccg cgccgtgccc tggaaaaggc ccagtatgcg
     3541 t
//
LOCUS       DAOPBQ010000397         3525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21421_length_3525_cov_4.472046, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000397 DAOPBQ010000000
VERSION     DAOPBQ010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21421_length_3525_cov_4.472046"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PKW60_11810"
     CDS             <1..507
                     /locus_tag="PKW60_11810"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPK00164.1"
                     /translation="LAGDGAMEVCASNLVTKQTHGSGQLHVEFLCPLMAEARDQARAN
                     SGVYVQGRYEVQVLDSFGDAPADNLCGGIYKIATPQMEASLPPGEWQTYDITFTAPKF
                     DEAGQKVQNARITVVHNGNVIHDGIELPECTAGGVSDQEAPEGVLMLQDHGNRVRYRN
                     IWFLPAQD"
     gene            648..1358
                     /locus_tag="PKW60_11815"
     CDS             648..1358
                     /locus_tag="PKW60_11815"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK00165.1"
                     /translation="MHLSFIIPAYNESRRIGDTLVKAHDYFSRQNYDWEMIVVDDGSS
                     DGTAGFVRRSFPGVRVIDYTPNQGKGHAVRAGMLAARGEYRFFSDADGSTPVEEIEKL
                     RARFSDGADVVIGSRTLPDADVQVRQAWHRQTMGRVFNVFVQLLAVKGFPDTQCGFKG
                     FSRAAAADIFPRMTIKGFGFDAEVLFIARKHGFRIDQVGVVWLNSPASSVHAVSDSAR
                     MLRDLVRIRLRDLRRLYD"
     gene            complement(1422..2351)
                     /gene="ftsY"
                     /locus_tag="PKW60_11820"
     CDS             complement(1422..2351)
                     /gene="ftsY"
                     /locus_tag="PKW60_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPK00166.1"
                     /translation="MAEERQGFLGRLRERLGKARTSVINGVKRVFAAHGALGDSFFDE
                     LEGVLIEADFGVDTALGIVETMRQEACSRRVERAEDLLDILKEDLVTLLSAGDHSLSW
                     DGADGPHVTLIAGVNGSGKTTTAGKLASHLKGQGKTVMLGAADTFRAAAVEQLTIWSE
                     RAGVPIVKHQDGADPAAVAFDAADAAQARRIDCLLLDTAGRLHTKVNLMEELKKIQRV
                     VGKRIPGAPHEVLLVLDATTGQNGLQQARIFTEALQVTGLVLTKLDGTAKGGIVVSIQ
                     KELGVPIKLIGVGEGVDDLQPFNAREFVEALFS"
     gene            complement(2457..3233)
                     /locus_tag="PKW60_11825"
     CDS             complement(2457..3233)
                     /locus_tag="PKW60_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPK00167.1"
                     /translation="MSHGVDNKYSGDSSFERIDADAVCEECGTVNPEGTLFCKTCGNN
                     LRDQRTRRMKGEGAREYLDTPAQTSRRVLVGLLTVFALLLLVWAAMPGNVQRLQERLT
                     RSMAEGMYAEGVDASILWKGPISSVLNGLAGELRQKPVTSEDIRRAEGAPASNAYAGR
                     YFLRRGTRPEDLVVGSAVVSETDGRLLFVAMLGDVEIRGIGDLKSSRYPSSRFTGIFV
                     DGRYISAHGFARPLDTGGFLCVGQQDNSDRTHEIMAYRIP"
BASE COUNT          674 a         1156 c         1027 g          668 t
ORIGIN      
        1 ctcgcgggcg acggcgccat ggaggtctgc gccagcaacc tcgtcacgaa acagacgcac
       61 ggctccggac agctccacgt cgaattcctg tgcccgctta tggccgaagc ccgggatcag
      121 gcccgagcca acagcggcgt ttatgtccag ggccgttatg aagttcaggt actggacagc
      181 ttcggcgacg caccggctga taacctgtgc ggcggcatct acaagatcgc cacgcctcag
      241 atggaggcat cgctcccgcc gggcgagtgg cagacctatg acatcacctt taccgcgccc
      301 aaattcgacg aggcgggcca gaaggtacag aacgcgcgca ttacagtcgt ccacaacggc
      361 aatgtcatcc acgatggcat cgaacttccc gaatgcacgg ccggcggcgt gagcgaccag
      421 gaagccccgg agggcgtcct catgctccag gaccacggca accgcgtccg ctaccgcaac
      481 atctggtttc tgccggcaca ggattgacac agcacggaac ctccgcctgc aggcggatac
      541 cggtgagaga agcatctcgc atccgcctca tgctatgctt gtgccgtgcc ctggcgtgac
      601 ctcgaaccgg acgaagcgcg cagcggttcg tcccgcggga aggactcgtg catctttcgt
      661 tcatcatacc ggcctataac gaaagccgcc ggatcggcga tacgctcgtc aaagcccacg
      721 actatttttc ccggcagaac tacgactggg agatgatcgt ggttgatgac ggaagctccg
      781 acggcaccgc gggatttgtg cgccgctcct ttcccggcgt gcgtgtgatc gactacacgc
      841 ccaatcaggg caaaggccac gcggtccggg caggcatgct cgcggcccgg ggcgagtacc
      901 ggttcttctc ggacgcggac ggctcgactc cggtagagga aatcgaaaaa ctgcgggccc
      961 gtttcagtga cggagcggac gtcgtgattg ggtcgcgcac cttgccggac gccgacgttc
     1021 aggtgcggca ggcctggcac cggcagacca tgggccgtgt tttcaatgtc tttgtccagc
     1081 tcctggccgt caaagggttt cccgacaccc aatgcgggtt caagggattc agccgcgcgg
     1141 ctgccgccga catctttccc cgcatgacca tcaaaggctt cggctttgac gccgaggtgc
     1201 tgtttatcgc gcgaaaacac gggttccgca tcgaccaggt gggagtggtc tggttgaact
     1261 cccccgcatc aagcgtgcac gcggtcagcg actccgcgcg gatgcttcgg gatctcgtgc
     1321 gcatccgcct gcgcgatctg cgccgcctct acgactgaga cagcatccgg atccgccggc
     1381 acgggtgaaa agaccggcgt gtttggccgc gcggaaaggc atcaggaaaa cagggcttcg
     1441 acaaattcgc gcgcgttgaa gggttgcagg tcgtcaacgc cttcgcccac gccaatcaac
     1501 ttgatgggaa cgcccagctc tttctggatg gagaccacga tacccccttt ggcggtgccg
     1561 tcgagcttgg tcagtaccag cccggtgacc tgcaatgcct ccgtaaagat gcgggcctgt
     1621 tgaagaccgt tttgcccggt agtggcatcc agaaccagca acacctcgtg cggcgctccg
     1681 ggaatacgct tgccgaccac gcgctggatc tttttcaatt cctccatgag attgacctta
     1741 gtatgcaggc ggccggcggt gtcgagcagc aggcaatcga tacggcgggc ctgagccgcg
     1801 tctgcggcgt caaacgccac cgccgccggg tcggccccgt cctggtgttt cacgatgggc
     1861 acgccggcgc gctcgctcca gatcgtcagt tgctccacgg ctgcggcgcg aaacgtatcg
     1921 gccgcgccca gcatgacggt cttcccctgg cccttcagat gcgaggccag ttttcccgcc
     1981 gtcgtggtct tgcctgaacc gttcactcct gcgatgagtg tgacatgcgg cccgtccgcg
     2041 ccgtcccacg acaggctgtg atcgccggcg gaaagcagcg tcacgagatc ttctttgaga
     2101 atgtcaagca ggtcttcagc acgctcaacc cggcggctgc acgcctcttg gcgcatcgtt
     2161 tcgacgatgc ccagggcggt gtccacgccg aaatcggctt cgatcaagac cccttcgagc
     2221 tcgtcgaaaa aagaatcgcc caaggcgcca tgggcagcaa atacccgttt gacaccattg
     2281 ataacggaag tgcgtgcctt gccgaggcgt tcgcgcagcc gccccagaaa accctgtctt
     2341 tcctcagcca ttcgaagatc tttctagcgt gtgggtgaag agcattcgct tatcgttgcc
     2401 ggaacggtaa tgatacgagg gaatggcgcc cgcggcaact ttctgcagcg gatccgtcag
     2461 gggatgcggt acgccatgat ttcgtgggtg cggtcgctgt tgtcctgctg gcccacacag
     2521 agaaatccgc cggtatcaag cggcctggca aaaccatgcg cgctgatgta ccggccgtcc
     2581 acgaagatgc ccgtgaaccg cgagctggga tagcggctcg atttcagatc gccgatgccg
     2641 cgaatttcca catcgcccag catcgccaca aacagcagcc ggccgtctgt ctcggagacc
     2701 acagccgacc caacgacaag gtcttcgggc cgcgtgcctc tccgcagaaa ataccggccc
     2761 gcataggcgt tcgaagcagg cgccccctca gcccggcgga tgtcctcact ggttaccggt
     2821 ttttgacgca actcgccggc cagtccgttc agaacactcg agatagggcc tttccacaag
     2881 atgctggcat ccacgccttc cgcgtacatg ccttccgcca tgcttcgggt gagccgttcc
     2941 tgtaaacgct gcacattgcc gggcatcgca gcccaaacca gcagcagcaa agcaaacacg
     3001 gtgaggagac ccaccagaac ccgccggctg gtctgcgcgg gggtatccag atactcgcga
     3061 gcgccttcgc ccttcatccg gcgcgtgcgc tggtcgcgca gattgttgcc gcaggtcttg
     3121 caaaacagcg tgccttccgg attgaccgtg ccgcattctt cgcagacggc atccgcgtcg
     3181 atccgttcga aggaagagtc accgctgtac ttgttgtcca ctccgtgact cacgaggttt
     3241 taccctttca ttcctggcct gttgcccttc tccaccaccc gaagaccaga tattccccgc
     3301 tgcggcattc cttccgctga ctgattttcg cacagtcagc cagatttgtc cagtaattcc
     3361 attgccggac accacgacca acgcgtcacc gtgacagcac ttacgccacg acccgcccgg
     3421 cggcgcgccg ccccgccgaa tcgagccggc gcctcaaata ttcaagcgat cccctgtcaa
     3481 gccatttgct aatcttaccg gggcgtatat cgtcgcgcca tttgg
//
LOCUS       DAOPBQ010000398         3507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21538_length_3507_cov_3.813152, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000398 DAOPBQ010000000
VERSION     DAOPBQ010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21538_length_3507_cov_3.813152"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1063
                     /locus_tag="PKW60_11830"
     CDS             26..1063
                     /locus_tag="PKW60_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPK00168.1"
                     /translation="MIELGASDLHIAVGVPPMVRVAGDLHPLAGYPALRQEDTQEIIY
                     TVMNEEQIATFEATKECDMSFGIDGLSRFRLNVFRDRGSVVAAFRSIPFEIRTADELG
                     LPRVVQDFAHRPLGLILVCGPTGSGKSTTLASILDRINRERSVHIITIEDPIEYLHSH
                     HRSVVNQREVNADTHSFGAALRHVLRQDPDVILIGEMRDPETIAAALTVAETGHLAFA
                     TLHTNDALQTINRIVDVFPASQQDQIRTQLSFVLEGVVVQQLIPRADGTGRVIALEVM
                     IPNTAIRSLIRSEKLEQIPSMIEIGTSEGMMTMNQSLFRLVRRNLISLEMAFKRSSDP
                     DGLQRLIEKGM"
     gene            1060..2661
                     /locus_tag="PKW60_11835"
     CDS             1060..2661
                     /locus_tag="PKW60_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK00169.1"
                     /translation="MSKDVAIVSTRLPLEGLNRRPWVVGLGRIGMLIVVTIGTYRLAE
                     RDSPGISWLPLFLLFGFITSFYSQYRLFRPEIPPLLLTWAQVLVDFGVVAATVGFTGG
                     ADSPFTFLFVIVILEGGLLLGQRQGFIFASLATLFTLIQTFQWFPAAGDFEVFTQWYG
                     FIVKGLGYYLTAFISGYWNMRVYRMQQFQHEILDNMSNGFLITDQNGIIVAQNKAAGR
                     ILQLSQSAATGRPVHEVLRVDSGVECPVLTALRTGRHFTSFEFHARTSGGAVILLGLT
                     TSPIHDSHGSLTGVIASFTDLTEMATIREELKRQDRLAAVGELSAGLAHEIRNPVAAI
                     RGAVDELKESIAAPAIAQRLADIAIRESDHLNRIVTDFLDFARRPEVRRETFSILESL
                     KEVTELLRDTCRDSGELAIETHFPAPDLLVSGHRSQIKQVFMNIAKNGIEAMREHGTL
                     DIRVIPDATSIEIRFDDEGPGIAPDELARIFEPFYTTKASGVGMGLPVCMRIVTAHDG
                     TLRVTPREGGGMSVSVRLPILRQEE"
     gene            2668..>3507
                     /locus_tag="PKW60_11840"
     CDS             2668..>3507
                     /locus_tag="PKW60_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK00170.1"
                     /translation="MSNRFSALVVDDEAGMRELLEIVLGNDGYDVTAAATVEDAITAL
                     NDRPYDAVITDLRMENDHKAGLRLLSWIKENAPTTPAIMITAYASMETAIEALKRGAA
                     DYIMKPFKNDELRLLIKRAIAQRNMNREIIALRKSQALRGAVDNIIGKSHAIEETREM
                     IRRVGMLPSTVAIHGESGTGKELVARAIHQLSERAARPFVALNCGGFPENLLESELFG
                     HKKGAFTGAVEDKEGLFVVADGGTLFLDEVGELSLSLQVKLLRVLDNQTIMPVGGTTT
                     IKVD"
BASE COUNT          726 a         1073 c         1031 g          677 t
ORIGIN      
        1 tcagcgtaaa agagatgctg gcaagatgat cgagttgggt gcaagcgact tgcacatcgc
       61 cgtgggggtg cctccgatgg ttcgtgtggc cggcgacctg cacccgcttg cagggtatcc
      121 cgccttacgg caggaagaca cccaggaaat catctacacg gtgatgaacg aagagcagat
      181 tgccacgttc gaagcaacca aagaatgcga catgtcattc ggcatcgacg ggctgagccg
      241 gtttcgcctg aatgtctttc gagaccgggg gtcggttgta gccgcctttc gttcgattcc
      301 gttcgagatc cgcaccgccg acgaattagg cctgccgcgc gtagtacaag actttgccca
      361 ccgcccgctc gggttgattc tggtctgtgg gccgacgggg agcggcaaat caaccacact
      421 ggcctcgatt ctcgaccgga tcaaccggga acggagcgtc cacatcatta ccatcgaaga
      481 ccccattgaa tacctccatt cccatcaccg atccgtcgtg aaccagcgcg aagtcaatgc
      541 ggatacgcac agtttcggcg cggcgctgcg ccacgtgctt cgccaggatc ctgacgtgat
      601 tctcattggt gagatgcgcg accccgaaac catcgcggcc gcgctgaccg ttgccgaaac
      661 gggccacctt gcgttcgcaa cactgcacac aaacgatgcg ctgcagacga tcaaccgcat
      721 cgtcgacgta tttcccgcaa gccagcagga tcagatccgc acgcagctct cgttcgtact
      781 ggaaggggtg gtggtccagc aattgattcc tcgcgccgac ggcacgggcc gcgtcatcgc
      841 cctcgaggtg atgattccga acacggccat ccgttcgctt atccggtcgg aaaaactgga
      901 gcagattccg tcgatgatag agatcggcac gtcggaaggc atgatgacga tgaaccagtc
      961 gcttttccgg ttggtgcgcc gtaatctcat ctctctcgag atggccttca aacggagcag
     1021 cgacccggac ggcctgcaac gactgatcga aaagggaatg tgagcaagga tgtcgccata
     1081 gtatccacgc ggctgcccct ggaaggcctc aaccgccggc cctgggtggt gggcctgggc
     1141 cgcatcggca tgctgatcgt ggtgaccatc ggcacctacc ggctggccga gcgcgacagc
     1201 ccgggcatct cctggctgcc tttgtttctg ctgttcgggt ttatcaccag cttttattca
     1261 caataccggc tgttccggcc ggagattccg cccctgctgc tgacctgggc ccaagtgctt
     1321 gtcgatttcg gtgtggtcgc ggccacggtg ggatttaccg gaggtgccga cagcccattc
     1381 acctttcttt tcgtcatcgt cattctcgaa ggaggattgt tgctcggcca gaggcaggga
     1441 ttcatctttg cctcgctggc cacgctattt acgctgatac agaccttcca gtggtttcct
     1501 gcggccggcg acttcgaagt cttcacgcag tggtacggtt tcatcgtcaa aggcctgggg
     1561 tactacctca cggccttcat cagcggatat tggaacatgc gggtataccg catgcagcag
     1621 ttccagcatg aaatcctcga caacatgagc aacggcttcc tgattaccga tcagaacggg
     1681 atcatcgtcg cgcagaacaa agcggcaggc cgcattctcc agctgagcca gagcgccgcc
     1741 acggggcggc ccgtgcacga ggtgctgcgc gtcgattcgg gagtggaatg ccccgtgctg
     1801 acggccctgc gcacaggacg gcattttacg agcttcgaat tccacgcccg caccagcggc
     1861 ggtgccgtga tactgctggg attgaccacc agccccattc acgattccca cggcagcctg
     1921 acgggcgtca tcgcctcgtt taccgatctc acggaaatgg ccacgatacg cgaagaactc
     1981 aaacggcagg atcggctggc tgcagtgggc gagctgtccg cgggcctcgc ccacgagatc
     2041 cgcaaccccg tcgccgccat ccggggggcc gtagacgagc tcaaggaaag cattgcggcg
     2101 cccgcgatcg cccagcggct ggcggatatc gccatccgcg aatccgatca cctgaaccgg
     2161 attgtgacgg atttcctcga tttcgcacgg cgtcctgagg tccggcggga aaccttttca
     2221 attctggaat cgctcaaaga ggtaacggaa ctgttacgcg atacctgccg cgatagcggg
     2281 gaattggcga tcgagacaca ttttccggcg cccgacttgc tggtttcggg ccaccgttcg
     2341 caaatcaagc aggtgtttat gaatatcgcc aagaacggga tcgaagccat gcgcgaacac
     2401 ggaacgctcg acatccgggt tatcccggac gccacgtcga tcgagatccg gtttgacgac
     2461 gaaggcccgg gtatcgcccc cgacgagctt gcgcgcatct tcgagccttt ttatactacc
     2521 aaggctagtg gcgtcggcat gggcctgccc gtatgcatgc gtattgtgac tgcccatgac
     2581 ggcactctcc gggtcacccc gcgcgaaggc ggcggcatgt ctgtcagtgt gcgacttccc
     2641 atcttacggc aagaggagta gcgttgcgtg tccaatagat tttcagcgct ggtggttgat
     2701 gatgaagcag ggatgcgcga actgctcgaa atcgtgctgg ggaatgacgg gtacgatgtc
     2761 acggcagcgg cgaccgttga agacgccata acggcactga acgaccggcc gtatgatgca
     2821 gtgattacgg atctgcgcat ggagaatgac cacaaggcag gcttgcgcct gctgtcctgg
     2881 attaaggaaa atgcgcccac cactccggcg atcatgatca cggcctacgc ctcgatggag
     2941 accgccattg aggcgctcaa gcgcggcgcc gcagactaca tcatgaagcc cttcaagaac
     3001 gacgaactgc gcctgctcat caaacgcgcc atcgcccagc gcaacatgaa ccgcgagatc
     3061 atcgccctgc gcaaaagcca ggcgctccgg ggcgcggttg acaatatcat cgggaaaagt
     3121 cacgccatcg aagagacccg cgagatgatc cgccgggtcg gcatgttgcc cagtaccgtc
     3181 gcgatccacg gcgaaagcgg cacgggcaaa gaactcgtag cccgcgccat ccaccagctc
     3241 agcgagcgcg ccgcccggcc cttcgtcgcg ctcaactgcg gcggcttccc cgaaaacctc
     3301 ctcgaaagcg aattgttcgg ccacaaaaaa ggcgcgttta ccggcgcggt cgaagacaag
     3361 gaaggcttgt ttgtcgtagc cgatggcggc acgctgtttc tcgacgaagt gggcgaactg
     3421 tccctgtcgc tgcaggtcaa attactgcgc gtgctcgaca accagaccat catgcccgtc
     3481 ggcggcacca ccaccattaa agtcgat
//
LOCUS       DAOPBQ010000399         3492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21653_length_3492_cov_4.931626, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000399 DAOPBQ010000000
VERSION     DAOPBQ010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21653_length_3492_cov_4.931626"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PKW60_11845"
     CDS             <1..1009
                     /locus_tag="PKW60_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00171.1"
                     /translation="EAYRGEPGAPLASKRRGWRWTWEAVVLALALILIGFAVILALPL
                     FTDSDWSLWGRRPAALACEFSIDPAQGKSLQVECRNIGDRPIDLYLPWPAALPPGLKQ
                     PDRSVGGLELQLRELGTNSFETYPVPSEWWLYNGRPLNAGEPVVVEPLLTVQLHLDLT
                     MVKSIGQGIDAMRVIAQRGDGEVFDTYETTFSAQPTSPFASGRPGSETHRPEMPAAAP
                     APAPTLPPAGPDMPESPAAPETIAPPSPEEPPAPKIFAQVSFLGSVGGKAALSIQLDA
                     ESRPRRTMVEVGDDIGGGWLVDAIERGAGQAVVLLHPATGRTVRVVQGAGETALTETA
                     E"
     gene            complement(990..3047)
                     /locus_tag="PKW60_11850"
     CDS             complement(990..3047)
                     /locus_tag="PKW60_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00172.1"
                     /translation="MRLLFTSAVSLVFACIPFCAGSGAVDLMSEAFGPIDAGDALGNG
                     RLTVTFSTTGRIASVHWPSPGYFDQITYALRAGEQGQLFVPEEDGLYWAVRTSDAFFR
                     ELDDDFGRCLHGRPSVTRLSIPQLEAQPACVVGGGGKTGAPSFFVHGERDLLVARFEL
                     SEAAPPCTVYWCANFTPCTRLLPEWPAADSMLDYANDFAVFTPDQGRTIVHFRPEKLR
                     GSDWNRARELAAAGGQAPGWQDFGEGVWIAQSPDQPPTGFECGVSGAASSACAQVKAG
                     ALSGQTAAVGDCDSAFEFSSGTPARGNAAASFTVYIAFGRTYAAAVENLGYARQRGYD
                     ALLRETVEQWQRRLGFSGPSLQPHERLLDVLLTCMDRDTGALVRMPVASPPLHLDIPR
                     TGVMGAYALSVAGRHDLVRQRVDFYLSAVRRQGAPGSPLGSLPAGLYANGSDAAPRLI
                     LDVEAVAWVLWLCEHHAAALPSDERAAFIEQAWPALELATDFLAGWSDPRTGLPRTAF
                     NPRTLRDDLSPELAIHTHMGLTCALALAATNGQDRAEWRQRAQELETLIRARYIGADG
                     FWQVERPERFWNISLVPANHPRWQSTLDAALDEVSALRIEPDRASELLLAALRIPPAQ
                     AGVKEKAIHLLESGRFDRAALPPQSGASVPDSYRAALQYVAWKEALRVTAPLANGSIL
                     PSP"
     gene            complement(3044..>3492)
                     /locus_tag="PKW60_11855"
     CDS             complement(3044..>3492)
                     /locus_tag="PKW60_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00173.1"
                     /translation="HPWLPVVAPLAPIEYAWAGRQGHIATVLGERAAVCLASDGPFWL
                     ATSQPALMNELLAIDSPGSQTAASAMLTINWKTAGKIAETLLRKAGELELIPEMNDQE
                     ANAYLGKYARSFGCLGRLRLEGSARGESVAFKGTLVSPEEEGNSMP"
BASE COUNT          649 a         1125 c         1135 g          583 t
ORIGIN      
        1 cgaggcgtac cgcggagagc cgggcgcacc gctggcaagc aaacgccgcg gctggcgctg
       61 gacctgggag gcggtcgttc ttgcgctggc cctgattttg attggatttg cggtgatact
      121 ggcgctgcct ttgtttaccg actcggactg gtcactctgg gggcgccgcc ccgccgcgct
      181 ggcctgtgaa ttttccatcg atcccgcaca gggcaagagt cttcaggtgg aatgccgcaa
      241 catcggggat cggcccattg acctgtacct gccctggccg gcagcgcttc cgcccggact
      301 caaacagccc gaccgttctg tgggcggcct tgaactacag ctccgcgaac tgggcacaaa
      361 ctcttttgag acgtatcccg tgccttcgga atggtggctg tacaatggcc ggcccctgaa
      421 cgccggagaa ccggtcgtgg tcgaacctct gttgacggtc cagctccacc tggatctgac
      481 gatggtcaag agcatcgggc agggcatcga cgcgatgcgc gtgatcgcgc agcgggggga
      541 cggtgaggta ttcgatacct acgaaaccac gttttcagcg cagcccacaa gcccgttcgc
      601 atccgggcgg cccgggtccg aaacgcatcg tcctgaaatg ccggccgccg cgccggctcc
      661 agcgcccaca ctgccgccgg ccggcccgga tatgccggaa tccccggcgg cgcccgaaac
      721 cattgcaccg ccatccccgg aagagccgcc cgcaccgaag atctttgccc aggtgagctt
      781 tcttggcagt gtgggcggga aggcggcgct gagtattcaa ctggacgccg aaagccgccc
      841 acgccgcacg atggtggaag tgggggacga cataggcggc ggctggctgg tcgatgcgat
      901 tgaacgcggc gcgggacagg ccgtggtgct actgcatcca gccacgggac gcaccgtgcg
      961 tgtggttcaa ggcgcgggag agaccgctct cacggagacg gcagaataga cccgttggcc
     1021 aaaggagcgg tcacgcgcaa cgcctctttc cacgcgacat attggagggc ggcccgataa
     1081 ctgtccggta cgctggcgcc gctctgcgga ggcagcgccg cccggtcaaa acgtcccgac
     1141 tccagaagat ggatggcttt ctctttcacg ccggcctgag ccggcgggat gcgcagggcg
     1201 gccaagagaa gctcactggc ccggtccggc tcgatgcgga gcgctgacac ttcatccagg
     1261 gcagcgtcca gcgtgctctg ccatcgcggg tggtttgcag gcaccaggga gatgttccaa
     1321 aagcgttcgg gccgctccac ctgccagaag ccgtcggcgc caatatatcg cgcacgaatc
     1381 agggtttcga gttcctgggc gcgctgccgc cattccgcac ggtcctggcc gtttgttgca
     1441 gccagagcca gtgcgcaggt caagcccatg tgggtgtgga tggcaagctc gggcgagaga
     1501 tcgtcacgga gggtccgcgg gttgaacgcg gtgcgcggca ggccggtgcg cggatcggac
     1561 cagccggcaa gaaagtccgt agccaattcg agcgccggcc aggcttgttc gataaaggcg
     1621 gcgcgctcat ctgaaggcag ggcagctgcg tgatgttcgc aaagccatag tacccaggcc
     1681 acggcctcga catcgaggat caggcgcggc gcggcgtcgc tgccattcgc gtaaagtcca
     1741 gcggggaggg aacccagcgg gctgcccggg gcgccctgcc tgcgcactgc gctcaaatag
     1801 aagtccactc gctgtctgac cagatcatgc cgccccgcga cggacagcgc atacgccccc
     1861 ataacgccgg ttcggggaat atccaggtgc agcgggggag aggcgaccgg catacgaacc
     1921 agcgcgcctg tgtcgcggtc catgcaggtg aggagcacgt cgagcagccg ctcatgcggc
     1981 tggaggctgg gcccggaaaa cccgaggcgc cgttgccact gctcaacggt ttcgcgcaac
     2041 aacgcgtcgt aacctctctg gcgggcgtag ccgagatttt cgacagccgc ggcatacgtg
     2101 cgcccaaacg cgatataaac cgtgaacgat gccgcggcgt tgccacgggc cggcgtgccg
     2161 ctcgaaaact caaacgcgga atcacagtcg cccactgcag ccgtttgccc gctgagggcg
     2221 ccggccttca cctgagcaca cgcagacgaa gcggcgccgg agacgccgca ttcaaacccg
     2281 gttggcggct gatccgggct ctgggcaatc cagacgcctt cgccgaagtc ctgccatccg
     2341 ggcgcctgac cgcctgccgc cgccagctcc cgcgcccggt tccagtccga gccccggagc
     2401 ttctccgggc ggaaatggac gatggtacgc ccctggtccg gcgtaaacac cgcgaaatcg
     2461 ttggcataat ccagcatact gtctgcggcc ggccattcag gaagcagccg cgtgcacggc
     2521 gtgaagttcg cgcaccaata caccgtgcag ggcggcgccg cctcgctcaa ctcaaagcgc
     2581 gcgaccagca gatcccgttc accatggacg aaaaatgagg gtgcgcctgt tttcccgccg
     2641 ccccccacga cgcaggcggg ctgggcttcc agctgaggaa tggaaagccg tgtcaccgac
     2701 ggccggccat gcaggcatcg gccgaaatcg tcatcaagtt cccggaaaaa tgcgtcgctg
     2761 gtgcggacgg cccagtacaa gccatcttct tccggcacaa aaagttgccc ctgctccccg
     2821 gcacgtaatg cataggtgat ctggtcaaaa taaccgggac tgggccagtg gacgctcgca
     2881 atccgcccgg tggtgctgaa ggtgaccgtt aatcggccgt ttccgagggc atccccggcg
     2941 tcaatcgggc cgaaggcctc gctcatgaga tccacagcgc cgctgcctgc gcagaacggg
     3001 atacaggcga ataccaatga cacggcggag gtgaagagga gtctcatggc atgctgtttc
     3061 cttcctcttc aggacttacc agggtgcctt tgaacgcaac ggattcgcct cgcgcggaac
     3121 cttcgagccg gagacggccc aggcacccaa atgatcgagc gtatttgccc agatacgcgt
     3181 tcgcttcctg gtcgttcatc tcaggaataa gctccaattc cccggctttt ctcagcaggg
     3241 tttcagcgat ctttccggct gttttccagt ttatggtgag catcgcgctg gcagcggttt
     3301 gcgagccggg tgaatcgatg gccaggagct cgttcatgag cgccggctgg ctcgtggcaa
     3361 gccagaaggg gccatcactg gccaggcaga cggctgcccg ctcacccagg acggtggcga
     3421 tatgcccttg acgccccgcc caggcgtatt caatcggtgc aagcggggca acgacgggca
     3481 gccacggatg ct
//
LOCUS       DAOPBQ010000400         3469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21816_length_3469_cov_2.880199, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000400 DAOPBQ010000000
VERSION     DAOPBQ010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21816_length_3469_cov_2.880199"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PKW60_11860"
     CDS             <1..335
                     /locus_tag="PKW60_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HPK00174.1"
                     /translation="LATEQRIPGLGNGVLQDILLAAKIHPKHKLATLTPPERDALFTA
                     VKSTLAAMTDQGGRDTEADLFGNPGGYRTRMSRYTVDRPCPDCESLIRKASFMGGSIY
                     CCPACQEI"
     gene            911..2008
                     /gene="ddlA"
                     /locus_tag="PKW60_11865"
     CDS             911..2008
                     /gene="ddlA"
                     /locus_tag="PKW60_11865"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPK00175.1"
                     /translation="MERKIRVGVVFGGKSTEHAVSLLSAQSIIRTLDPERFEMVFIGI
                     DKEGRWALCDNARPLLHADDPEQIALCPSAHPVAVVPGGSEQRLSVLTDSHRATPIDV
                     VFPVLHGPLGEDGTVQGMLKLANLPFVGAGVLGSAVGMDKDVAKRLLRDAGIPVANFL
                     AVNARHAVTLTYDEVVTRLGSPFFIKPANAGSSVGVSKVTNTEDYATALREALKFDTK
                     VLIEEFIEGREIECAVLGNDEPTASCLGEIIPTHDFYSYEAKYLDEHGALLKIPAEIE
                     QGTGEHLRRLAVQTFQTLCCEGMARVDFFLRPNGKAIVNEINTIPGFTSISMYPKLWE
                     ASGLPYGKLVERLIDLALERHEREQRLQTSI"
     gene            2138..2419
                     /locus_tag="PKW60_11870"
     CDS             2138..2419
                     /locus_tag="PKW60_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00176.1"
                     /translation="MTADAKAGATVMGSARHIDERHLGKTVAAFVDGHVETRPAQGLP
                     GGGVLLNKIAFHSDRDGNYQIYVMNADGTGKTRLTNNTASDYIYQGGWR"
     gene            complement(2981..>3469)
                     /locus_tag="PKW60_11875"
     CDS             complement(2981..>3469)
                     /locus_tag="PKW60_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK00177.1"
                     /translation="PQPDPLTEPEEPEEPTEQQTPMTVDLTDLLRDRLASMDARLART
                     YSGTGLGLSLVKQLAELHGGTVAVESAPGQGSRFTITLPWEACPAPADELDAPAPDAP
                     ARRGPAMPRGDPDGRADARPGRPASHAAPARPARLRRPPPSSPSPRWPCPATATAAWP
                     PA"
BASE COUNT          709 a         1039 c         1060 g          661 t
ORIGIN      
        1 tcctcgccac cgaacagcgc atccccggcc tggggaatgg cgtcctgcaa gacatccttc
       61 tcgcggcgaa aatccatccc aagcataaac tcgccaccct gacgccaccg gaacgtgacg
      121 cgctgttcac cgcggtgaaa tcgaccctcg cggccatgac cgaccagggc gggcgcgaca
      181 cggaagccga cctcttcggc aatcccggag gttaccggac caggatgagc agatatacgg
      241 tggaccgacc ctgcccggac tgcgagagcc tcatccgcaa ggcaagcttc atgggcggca
      301 gcatctattg ctgcccggca tgccaggaaa tatagtgccg tgcgtttcag ggataatcat
      361 ccaatccttg gtacgataga agatacgata gaatcagttt cgaagaattt ctggtaggca
      421 ttcatccctg tttcctcgga gaattttcga tttcaggcac ttttcctcca ccatatcttc
      481 gcctcaaacc gcctctcggc ggtttgttgg ctcagggcga cctcggacca acacgccacg
      541 ttctacgcgc aaatccgcct gggtgaacac ctttcggtac cgaaacctct ggacgatcca
      601 aacagcgttt gccccactac aaagccctaa atgggccaat tctccctctg attctctgga
      661 acctccggac gacccctcac cccatccaga ggttggtggg tggggctgcg gacatcatcc
      721 ggaggtcaga aggggtatcc ctgtactgct atcagtatcg ccgtgacaaa aagcactgaa
      781 gccatataca attactggta gtgctgttat ccggccatcg agtagacact catgcgcttt
      841 gccgagaagg cagatgagag agttcaggca aagtatgctc atcacagtca tctgagtcag
      901 gagtagaacc atggaacgaa agattcgtgt tggggtggtg ttcggcggga aatcgaccga
      961 gcatgcggtg tcgttattgt cggcgcagag tattatccgc accctggacc ccgagcgatt
     1021 tgagatggtg ttcatcggta tcgataagga ggggaggtgg gcattatgcg acaacgcccg
     1081 gccgttgctg catgcggatg accccgagca gattgccctt tgtccttcgg cacatcccgt
     1141 ggccgtcgta cctggcggta gtgaacagcg gttgtccgtc ttgaccgata gtcatcgcgc
     1201 aacgcccatt gatgtggtgt tcccggtgct gcatggtcca ctgggtgagg atggcacagt
     1261 gcaggggatg ttgaaattgg caaacctgcc ttttgtcggt gccggggtgc tcggttcggc
     1321 agtcggcatg gataaggatg tggcgaaacg gctgttgcgt gacgccggta ttcccgtggc
     1381 caatttcctt gcagtgaacg cgcggcacgc ggtgacgcta acctatgatg aggtcgtgac
     1441 gcgactcggc tcgccgtttt tcatcaagcc cgccaatgct ggttcttcag tcggtgtaag
     1501 caaggtaacg aacaccgagg actatgcaac cgcgctacgg gaagccctga agttcgacac
     1561 aaaagtgctc atcgaagaat tcatcgaggg tcgggagatc gagtgtgctg ttctcggtaa
     1621 tgacgagcca acggcatcct gcctgggtga aatcatcccg acccatgact tttacagcta
     1681 cgaggccaaa tacctggacg aacatggcgc gttactcaag attccagcgg agatcgagca
     1741 gggcacggga gagcatctcc ggcgactggc cgtacaaacg ttccaaacgc tctgctgcga
     1801 aggcatggcg cgcgtggact ttttcctgcg ccccaacggt aaagcgatag tcaacgaaat
     1861 caacaccatc cccggcttta ccagcatcag catgtatcca aaactttggg aagccagcgg
     1921 cctgccatac ggcaagctgg tggagcgtct cattgatctc gccttggagc ggcatgaacg
     1981 tgagcaacgg ttacagacat cgatctgata gattaagggg aaatgcggga ggcgttagat
     2041 gaaagtagtc tataagatca cctctccgga atacgccttc tcccaccggc tgttcggccg
     2101 ggcgctgggc gaggtgaccg ccccggagac ctgaacgatg acggccgacg cgaaagccgg
     2161 cgccaccgtg atgggctccg cccggcatat tgatgagcgc cacctgggca agacggtggc
     2221 ggccttcgtg gacggccacg tggagacccg cccggcccaa ggcttacccg gcggcggcgt
     2281 gctgctgaac aagatcgcct ttcactcgga ccgcgacggc aactatcaga tctacgtgat
     2341 gaacgccgac ggcacgggga agacccggct gacgaataac acagcgtctg actacatcta
     2401 ccagggcggc tggcgctaaa taccgcccgg ctgtttcaac tgacctgcta cagtgcaaag
     2461 ctgaggatgt catcaaatcc atacgggagt aatactagtg caaatcatga acatgctctt
     2521 ccactgtgct gtccgcgcag gcgaatggcc gcgctcccag ggaaaccgtg cccaacagct
     2581 ccaatgcgga acagcattct tccgatttgc cctagtagtc cggcgaagaa caccgcatgt
     2641 ttgccggtat catcagcgct gtccagcata ggacgccccc cacctggcga tggaacggag
     2701 ggctcacctc tcccccaccc cctcccctac ctgggaaggg gagcgacgga ttggtcggct
     2761 tccctgtcgg gagcagtgcc ggaggatgac acacgcgcag gatgatggac acctctgcag
     2821 tagcgcaccc tgcacctggc caccggccca acaggccacg catcgttgct tgcttgctga
     2881 cagcgcggct gagacggtca ggacccttgc gccacccccc gcgcgcgcgc aatcgcccgg
     2941 gcgatgcgct ccaggctgcg gggtttgctg aggtagtcgg tcatgccggc ggccaggcag
     3001 cggtcgcggt cgccgggcag ggccagcgcg gtgatggcga tgatgggggg gggcggcgaa
     3061 gccgggcagg gcgcgcaggc gctgcgtggc ttgcaggccg tccaggccgg gcatctgcac
     3121 gtccatcagg atcaccgcgg ggcatcgccg gcccccgacg cgccggggcg tcgggggccg
     3181 gcgcgtccag ctcatccgcc ggggcggggc aggcctccca gggcagggtg atggtgaagc
     3241 ggctgccctg tccgggcgcg ctctccaccg ccaccgtgcc gccgtgcaat tccgccagtt
     3301 gcttcaccag cgacaggccg aggccggtgc ccgagtaagt ccgcgccagg cgggcatcca
     3361 tcgaggcgag ccggtcgcgg aggaggtctg ttaaatcaac ggtcataggc gtttgttgct
     3421 ctgtcggttc ctccggttcc tccggttccg ttagcgggtc aggttgcgg
//
LOCUS       DAOPBQ010000401         3466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21831_length_3466_cov_5.110232, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000401 DAOPBQ010000000
VERSION     DAOPBQ010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21831_length_3466_cov_5.110232"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PKW60_11880"
     CDS             <1..491
                     /locus_tag="PKW60_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HPK00178.1"
                     /translation="AAAAAVAAARVQSAPPAACPGSAQRMFAPRPPRAQEGVAAESAL
                     THWPIQLHLITPDAPVFRNADILIAASCTAFACGRFHPELLEGRSLIIACPKLDRLDG
                     YLDKLTALFLDAEPRSVTVARMVVPCCNGLTFMVKQAREMASSDVPVDEVIIGVEGER
                     LS"
     gene            complement(519..1697)
                     /locus_tag="PKW60_11885"
     CDS             complement(519..1697)
                     /locus_tag="PKW60_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPK00179.1"
                     /translation="MDWETCIHDEGIRRKAFPVAGECVFLAHAEVAPLPQASADLMSE
                     FLDRASHRKQAGAWTETWVEAARRFAAEMLGAESSEIALLGPTALGLNLVAAGLPWRP
                     GDEVVYYPDDYPANVYPWAALSARGVKPVALRPDVPGVITWDLIASALTERTRLVSLA
                     TAHFLTGFRIDIDTIGRNLHQRGILFCLDAIQTLGAFPLNVEHVDFLCADSHKWLLGP
                     AGAGILYVSRDRFDTLQPALLGSWNVVSPNFIAQDSIAFYGGARRYEPGILNLPGIVG
                     MAASLELLLSVGVDAIAERILHLRRGLLERLRALGYRHCLDMNSWPAETAEADLSGII
                     TVTHPEKDMAEVAHRLQTADIVVSLRYNRAGDAFLRFSPHFYNTEAELEHVAGVLTRC
                     "
     gene            1967..2512
                     /locus_tag="PKW60_11890"
     CDS             1967..2512
                     /locus_tag="PKW60_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00180.1"
                     /translation="MMTGHIPDPLQTKRIEPPPDMSGRAFGETGPAPGAGFRDDERQI
                     HAGFGDNTISIPALVAHTVDRNFEEARKMLPSLEELRAAQQARAEERRAEAQERLEAQ
                     RATLGASSAQEPVIRVDFQRAESQARAQTQAAASAPNSSAAPAGIGTPAFAKGPDTSV
                     RAACRAFVFAPLALARFDVRG"
     gene            2753..3298
                     /locus_tag="PKW60_11895"
     CDS             2753..3298
                     /locus_tag="PKW60_11895"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HPK00181.1"
                     /translation="MLVLLLLLAGLGHAAAAQDVDFREAFSAGRWRVETQGFAALYSG
                     KTDRQGDKFATFSVEYEMPVTSHFCFGLRGYPVLVYWPHRTSDDTLYGVAGGVTGRYY
                     FDKDSYRGWFAEGGGSVLWHSRYFRANGSRINFMLEAGVGYQFAQDWHVAFKFNHMSN
                     ASTHTDNAGVNALGLGVGYTF"
     gene            complement(3286..>3466)
                     /locus_tag="PKW60_11900"
     CDS             complement(3286..>3466)
                     /locus_tag="PKW60_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00182.1"
                     /translation="KILPIVRELAHMRLQLRHGQGKHVLDQTRGLCVRALELVNEIRA
                     QTWSALASLDGNQKV"
BASE COUNT          607 a         1061 c         1146 g          652 t
ORIGIN      
        1 ccgccgcggc tgcggcagtg gccgcggcgc gcgtgcaatc ggctccgcct gccgcgtgcc
       61 ccggttccgc ccagcgcatg ttcgctcccc ggccgccccg tgctcaggag ggcgtggctg
      121 cggagtccgc gttaacccac tggcccatcc agctccacct gatcacgccc gatgccccgg
      181 tcttccgaaa cgcggacatc ctgatcgcgg cctcgtgcac ggcgtttgcc tgcggccgct
      241 tccatcccga attgctcgag ggccgttccc tcatcatcgc ctgccccaaa ctggaccggc
      301 tcgacggcta tctcgacaag ctcaccgcgc tgttcctcga tgcggagcct cgcagcgtga
      361 ctgtggcccg catggtggtg ccctgctgca acggactcac gttcatggtg aaacaggccc
      421 gcgaaatggc cagcagcgac gtgcctgtcg acgaggtgat catcggcgtg gaaggtgaac
      481 gcttgagttg atgggacaat gcccggcggt gcggcgtttc agcagcgcgt cagaacgccg
      541 gcgacgtgtt ccagctcggc ctccgtgttg taaaaatggg gtgaaaaacg gagaaacgcg
      601 tcgcccgcgc ggttgtagcg aagcgatacg acgatgtctg ccgtttgcag gcgatgggca
      661 acctcggcca tgtctttttc cgggtgggtc acggtgatga tgcccgagag atccgcctcg
      721 gcagtttcag cgggccagct gttcatatcg aggcaatggc ggtacccgag tgcgcgcagc
      781 cgctccagca gcccacggcg caggtggaga atccgttcgg cgatggcgtc gacgccgacc
      841 gacaggagca gttcgagcga cgcagccatg cccacaattc ccggcagatt cagtatcccc
      901 ggctcgtagc gccgcgcacc accgtagaag gcgatgctgt cctgggcgat gaagttcggc
      961 gaaacgacat tccaggagcc gagcagggcg ggctgcaggg tgtcgaacct gtcgcggctt
     1021 acatacagga tgcccgcgcc tgcggggccc agcagccatt tgtgcgagtc cgcgcagagg
     1081 aaatcgacgt gttccacatt cagcggaaac gcgcccagcg tctggatggc atccaggcag
     1141 aacaggatgc cgcgttggtg caagttgcgg ccgatggtgt cgatgtcgat gcggaacccg
     1201 gtgaggaaat gcgccgtggc gagggatacc agccgcgtgc gttcggtcag cgcggatgca
     1261 atcaggtccc aggtgatcac gccgggcaca tcgggccgga gcgccaccgg cttcacgccg
     1321 cgcgcgctga gtgcggccca agggtagacg ttggccgggt aatcgtcggg gtaatagacg
     1381 acctcgtcgc cggggcgcca tggcaggcct gcggcaacga ggttcaatcc gagcgcggtc
     1441 ggcccgagca gcgcaatctc gctggattcc gcgcccagca tctccgccgc gaacctccgg
     1501 gcggcttcga cccaggtctc ggtccaggcg cccgcctgtt tgcggtgtga ggcccgatcc
     1561 agaaactcgc tcatgaggtc ggcagaagct tgcggaagag gcgccacctc cgcgtgcgcg
     1621 agaaagacgc actcacccgc caccggaaag gctttgcgcc ggattccttc gtcgtgaatg
     1681 caggtttccc aatccatggg gaaagtgtac tggtttgccg cgcgctttgc cagacccggc
     1741 tgcgaaccgt ctttaaatct accggaacgt cagggaagca atgggtttag agtggcccgt
     1801 gctatgtgaa acaggccccc gctgcaatca ttctaaaaca ttggcataaa tgcccttacg
     1861 tcgattcagg cagccccttt ccttgacagg cgcgcggtcg ggtgggataa tttcaggtgt
     1921 tcaggtagtg cgggctgaaa atgcacatgg ggtgtatgcc atgggcatga tgacaggtca
     1981 catacccgac ccgctccaga cgaagcgcat cgagccgcca ccggatatga gtggacgggc
     2041 ttttggggaa acggggccgg cacctggagc gggcttccgc gacgacgaac gtcagattca
     2101 cgcggggttt ggcgacaata ccatctccat cccggcgctg gtcgcgcata ccgtggatcg
     2161 caattttgaa gaagcccgca aaatgctccc ctctctcgag gaattgcggg cggctcagca
     2221 ggcgcgggca gaggagcggc gcgcggaggc tcaggaacgg ctcgaagcac agcgggcaac
     2281 gctgggcgcg agcagcgctc aggagcccgt tatccgggtc gatttccagc gggcagaatc
     2341 gcaggcacgc gcgcaaaccc aggccgctgc cagcgccccg aattcatctg ctgcgcctgc
     2401 cggcattggc accccggcgt ttgcgaaggg cccggacacg tcagttcggg ccgcgtgccg
     2461 ggcctttgtt ttcgcgccgc tcgccctggc ccggtttgac gtgcggggct agtcttttcc
     2521 ggaaggccgg cgcgcgggag gcgaaaggcc cgtgcctcct gtttcgtcag aaagcctctc
     2581 cacattgccg ctcgcatcgc cgcgaatgaa acgcgcgcgg ggccggcggt atcgcgtttt
     2641 ttttgacatg ccggagtgtg ttcagtagac ttcgaagcga tctgtcgaag tggtttattc
     2701 gagttacccc tgcctggaga aggttcaacg gacgatgaga ttttgcgcat ctatgctggt
     2761 tttgctgctg ttactggccg gcctgggcca tgccgccgcg gcgcaggatg tcgattttcg
     2821 tgaggcgttc agtgcaggcc ggtggcgtgt ggagacgcag ggctttgccg ccctgtattc
     2881 gggaaagacg gatcgccagg gcgacaagtt tgccacgttc tccgtggagt atgagatgcc
     2941 cgtgaccagc catttctgct tcgggctgcg cgggtatccc gtgcttgtgt actggccgca
     3001 ccgcacatcc gacgatactt tgtatggcgt ggcaggcggg gttacgggcc ggtactactt
     3061 tgacaaagac tcgtaccgcg gctggttcgc ggaaggcggg ggcagcgtgt tgtggcattc
     3121 gcggtatttt cgcgcgaacg ggtcacggat taatttcatg ctggaggccg gcgtgggcta
     3181 ccagttcgcg caggactggc atgtcgcgtt taaattcaac catatgtcca atgccagcac
     3241 gcacaccgac aacgccggcg tgaacgcact cgggctgggc gtcggctaca ctttctgatt
     3301 cccatccaag gatgccaggg cgctccaggt ctgggcccgg atctcgttga ccagttcgag
     3361 cgcccgcacg cagagccccc gcgtctgatc caggacgtgt tttccctggc catggcggag
     3421 ctgcagacgc atgtgcgcga gttcgcgcac gattggcaga attttc
//
LOCUS       DAOPBQ010000402         3457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21898_length_3457_cov_3.812169, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000402 DAOPBQ010000000
VERSION     DAOPBQ010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21898_length_3457_cov_3.812169"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1525
                     /locus_tag="PKW60_11905"
     CDS             62..1525
                     /locus_tag="PKW60_11905"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPK00183.1"
                     /translation="MRLKELSKWLNAERALETDAVITSAVEDSRRVTPGAVFVAIQGE
                     TSDGHEHATDAAGRGAVAIIGGRKGLEKLAGLPYVYVPNPRQTLGLLAHRLAGDPSKD
                     MTVIGITGTNGKSSTALLTQHILKTCGCPAACFGTLGYSIAGVTLQAKHTTPFGDELA
                     AIFRRAREARQSHVVMEVSSHALAQERVAGIDFDVAAFTNLTQDHLDFHQDMEAYGAA
                     KLLLFERLEGPGRFTVVNREDPAADAFIRASRVACFTYGKGADVRAVGVRAEGPGTGF
                     RVESPWGEAEVTVPLVGKHNVLNTLCAIAICGGLELPLSAVAAGLASAPVIPGRFERV
                     SAGQDFEVIVDYAHTEDGLRNVLKATRALTRGNVIVVFGCGGDRDRGKRPKMGAAAGE
                     LADYAIITSDNPRTEDPEQILLDIEVGIRRIGKHRPEDYNLILDRAEAIRLGIAMAKS
                     GDAVLIAGKGHEDYQILGTERIHFDDREVARAALEGS"
     gene            1531..2874
                     /gene="murF"
                     /locus_tag="PKW60_11910"
     CDS             1531..2874
                     /gene="murF"
                     /locus_tag="PKW60_11910"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPK00184.1"
                     /translation="MAWTYSLAELAAAIECKPVAETATFSSISTDTRTLRAGDVFFAL
                     TGANFDANAFVGEAFAKGACAVVASRAHSSGPCLVVDDPLQALQRFAAWHRARFSLPV
                     LAITGSCGKTTTKDLIAELLAPRFPVAKTPGNLNNDIGVPLSLMEIDAESGFAVIEMG
                     ANHQGEIAALCGMARPTEAAITMIAPAHLEGFGSIENVAAAKAEIVRGLGSSGVFYVN
                     TGDPRCVAIGETFAGECVRFGESGDVTLRRREFLPSGDTLLEIDPVGPLALPLACPAH
                     VTNVLLAVAVGLRHGATEFEGPLRKALAAATRFQVLRIGPWVVLDDSYNANPASMAAA
                     LEALALRPGPGLRVAALGDMLELGKASRALHRELGRTAAAAGVQHLFARGAFANEVVA
                     GALKEGCPHACAIESHADIAAAVHALEPEGGTLLVKGSRGMRMERVFESLKALYG"
     gene            2891..>3457
                     /locus_tag="PKW60_11915"
     CDS             2891..>3457
                     /locus_tag="PKW60_11915"
                     /inference="COORDINATES: protein motif:HMM:NF022030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00185.1"
                     /translation="MLYYLGEWLVPFVGAFNVLTYITVRAGGAAFSAFLITLLIGPRL
                     IHRLRLLKLGQYIREDHVQDLHALHKSKAGTPTMGGTLIIFSAVASLVLWGRFSNRLL
                     LLATVVLCVLGAIGFLDDYIKIRRKHNMGLTAKAKFAGQILVGLAVGLYVLYRPVTLD
                     ATHLSERDVTDWRALMTELFAGEGMRPGT"
BASE COUNT          619 a         1023 c         1179 g          636 t
ORIGIN      
        1 cccctggccg aggtgaccct ctgccggctc cggttttcca acgaacgcaa gaaggcggaa
       61 gatgagactc aaggaactgt cgaaatggct aaacgctgag cgtgcgctcg agacggatgc
      121 cgtgattacc tcggcggtgg aagattcgcg ccgggttacg cccggcgcgg ttttcgttgc
      181 gatccagggg gaaaccagcg acgggcacga gcacgccacc gacgcggcgg gccggggcgc
      241 agtggcgatt atcggaggcc ggaaagggct ggagaaattg gcggggctcc cctatgttta
      301 cgtgccgaat ccgcgccaga ccctgggtct gctggcccac cggctcgcgg gcgatccctc
      361 gaaagacatg accgtcatcg gcatcacggg caccaacggc aaatccagca cggcgctgct
      421 gacgcagcat atcctgaaga cctgcggctg cccggcggcc tgtttcggca cactgggcta
      481 ttcgattgcc ggcgttacgt tgcaggcgaa gcacaccaca ccgttcgggg acgagctcgc
      541 cgcgatattc cgccgggcgc gggaggcgcg gcagagtcat gtggtgatgg aagtcagttc
      601 gcacgccctg gcgcaggaac gcgtggccgg catcgatttt gacgtggccg catttaccaa
      661 tctgactcag gatcatctgg acttccacca ggacatggaa gcatatggag cggcgaagct
      721 gctgctgttt gaacgtctgg aaggtcccgg gcggttcacg gtggtgaatc gggaagatcc
      781 ggcggcggac gccttcatcc gcgcctcgcg ggtggcctgt tttacctacg gcaaaggcgc
      841 cgacgtgcgg gcggttgggg tgcgcgcaga gggcccgggc accgggttcc gtgtggaatc
      901 tccgtggggc gaggcggaag tcaccgttcc gttggtggga aaacacaatg tgctgaacac
      961 gctgtgcgca atcgccatct gcggcgggct ggaattgccg ctttcggcgg tggccgccgg
     1021 gctggcgagc gcgccggtga tccccggacg ttttgagcgg gtgtcggcgg ggcaggattt
     1081 cgaagtcatt gtcgactatg cgcacaccga agacggactg cgcaatgtgc tgaaagccac
     1141 gcgcgcgctc acccggggca atgtgatcgt ggtgtttggg tgcggaggag atcgcgatcg
     1201 cggcaaacgc cccaaaatgg gcgcggcggc tggagagctg gccgattacg ccatcatcac
     1261 ctccgacaac cctcgaactg aagatcccga acagatcctg ctggatatcg aggtcggtat
     1321 ccggcgcatc ggaaaacatc gccccgaaga ctacaacctg attctggacc gcgccgaagc
     1381 gattcgactg ggcatcgcga tggcaaaatc cggagatgcc gtgctgatcg cgggcaaagg
     1441 gcacgaagac taccagatcc tgggcacaga gcgcattcat tttgacgacc gtgaagtcgc
     1501 gcgcgcggca ctcgaaggga gttgagccgc atggcctgga cgtattcact ggccgagctg
     1561 gctgccgcga ttgaatgcaa acccgtcgcg gaaacggcaa cattctcgtc gatctcgaca
     1621 gatacccgga cgctccgggc gggcgatgtc ttttttgcgc tcaccggggc aaatttcgac
     1681 gcgaacgcgt tcgtcggcga ggcttttgcc aaaggggcct gcgcggtggt cgcctcgcgg
     1741 gcgcacagct ccggcccctg cctggtcgtt gacgaccccc tgcaggcgct ccagcgcttt
     1801 gccgcctggc atcgcgcccg tttctcgctg ccggtactcg cgatcacggg ctcctgcggg
     1861 aaaaccacca caaaagacct gattgcggaa ttgctggccc cacgcttccc tgtcgcgaaa
     1921 acgccgggaa atctcaacaa tgacatcggc gtgccgctgt cgttgatgga gattgacgca
     1981 gaatccggtt ttgccgtaat tgaaatgggc gcgaatcatc agggcgagat cgcggcgctg
     2041 tgcggcatgg cacggccaac ggaggcggcc atcaccatga tcgccccggc gcacctcgaa
     2101 ggattcggca gcattgagaa cgtagccgca gccaaggcgg agatcgtgcg cggcctggga
     2161 tcgtcaggcg ttttctacgt caacaccggc gatccgcggt gcgtggccat tggcgagact
     2221 ttcgcggggg aatgcgtccg gtttggcgag tcgggagacg ttacactgcg gcgccgggaa
     2281 ttcctgccgt cgggcgatac ccttctggag atcgatccgg taggcccgtt ggcgctgcct
     2341 ctggcatgcc ctgctcatgt gaccaacgtg ttgctggcgg tggccgttgg actgcggcac
     2401 ggcgctacgg aatttgaagg gccgctccgc aaggcgctgg cggcagcgac ccggttccag
     2461 gtcttgcgca tcgggccgtg ggtggttctg gacgattctt acaacgcgaa cccggcgagc
     2521 atggcggccg cgctggaagc cctcgcgctc cggccaggcc ccggactccg ggtggcagcg
     2581 ctgggcgata tgcttgagtt gggcaaggcg tcgcgcgcac ttcatcggga attgggccgg
     2641 actgccgcgg ctgcgggcgt ccagcacctg ttcgcgcggg gcgcattcgc gaacgaggtt
     2701 gtggccggtg cgctcaaaga aggctgcccc catgcatgcg ccatcgagtc tcacgctgat
     2761 atcgcggcgg cggttcacgc cctggagccg gaaggcggaa ccctgctggt caaaggctcg
     2821 cgcggcatgc gcatggaacg tgtgttcgaa tcgctaaagg ctttatacgg ctgaaagacg
     2881 aaggagcggc atgttgtatt acctgggaga gtggctggtg ccgttcgtcg gcgcgtttaa
     2941 cgtgctgacc tacatcaccg ttcgggcggg gggtgcggcc ttctccgcct ttctgatcac
     3001 gctgctgatc ggtccgcgcc tgattcaccg gctgcggctc ttgaaactcg gccagtatat
     3061 ccgcgaagac cacgtgcagg atctgcatgc gctgcacaaa tccaaggccg gcacgcctac
     3121 catgggcggg acgttgatca ttttttctgc cgtggcgtca ctggtgttgt ggggccggtt
     3181 cagcaaccgc ctgttactgc tggccacggt ggtcctgtgc gtgctgggtg cgataggctt
     3241 tctggacgac tacataaaaa tccggcgcaa acacaacatg gggctgacgg cgaaggcgaa
     3301 attcgcagga cagatcctcg tgggactggc ggtcggcctg tatgtgctct accggccggt
     3361 caccctggac gcgacccacc tgtcggaacg cgatgtgacg gattggcgcg cattgatgac
     3421 cgagctcttt gcaggggaag ggatgcgccc gggcaca
//
LOCUS       DAOPBQ010000403         3457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21900_length_3457_cov_3.648148, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000403 DAOPBQ010000000
VERSION     DAOPBQ010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21900_length_3457_cov_3.648148"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PKW60_11920"
     CDS             <1..414
                     /locus_tag="PKW60_11920"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPK00186.1"
                     /translation="PAAKEAAPEPPVQTAAAPDTAPPRDTAAPPVSIDKIELPPENLP
                     PAAAAPAQTPFYSVQLIAFSSANRAKADAYAKEIRENAGLDVELEISTDGKLIRLYVG
                     QYATRDDAVKACQELKKQERFSQAFVPQEARKPKN"
     gene            460..1335
                     /locus_tag="PKW60_11925"
     CDS             460..1335
                     /locus_tag="PKW60_11925"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HPK00187.1"
                     /translation="MKVSVIGPGAMGCLFAARLAHAGIQTTLIDYKPDRAKRLNASGI
                     CVESDTEPLTATPLVATEIPDGQDLLMVFVKSHVTPTLTLPAGVPVLTLQNGLGNTET
                     LCSAVGSANVLAGVTHEAAMLNSEGHVTHTAAARTVFGSWTSCPTDAAEKAMDAAGFQ
                     CDVTAAPGQTLWEKVAASSAIYPLTAMLNIHNGALLEVPEVRQLMRDLVVESVKVAST
                     EGYRFPYSLVEEAEASCQKFPQCISPMLQDIRAGRRTEIESLCGEIVRKAQVAALPVP
                     RTRVIYQLIRGMESR"
     gene            1332..2033
                     /locus_tag="PKW60_11930"
     CDS             1332..2033
                     /locus_tag="PKW60_11930"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPK00188.1"
                     /translation="MSPPELSYDSPETFTFRQRMLLTLGCPLLAGAYKALCVTCRQER
                     RGEELFQRSLASGQHVFVAFWHETLGLAAWLHRNTGFHTLTSYSFDGEVAARVVRSFG
                     LYAVRGSSSRGGYEALRQMQRAVDKVPAVGFTLDGPTGPRRVAKPGAAILAIRTGVPI
                     VPLALAASSCWRLHSWDQLIIPKPFGRILSIYGDPILPPPKGAPKAIEQLRSEVERAL
                     NRLHASLEEELGQES"
     gene            complement(1994..3079)
                     /gene="rlmN"
                     /locus_tag="PKW60_11935"
     CDS             complement(1994..3079)
                     /gene="rlmN"
                     /locus_tag="PKW60_11935"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPK00189.1"
                     /translation="MKIDITDLLPDELAEALDLKVYQARQLFRWVHAKQVFDLNKMTD
                     LSKSMRDELRERITLPQIRPLQTLASSEAGAATRKQLFELHDGTTVEAVAIPEGERLT
                     LCLSSQVGCAVRCVFCATGLSGYVRSLSPGEIVEQALHLLRGRDMGGRTPNIVFMGMG
                     EPFRNYDAVAKAIRMLMRKDGLNIGARKITVSTAGDVPGIERFAAEKWQVRLSVSLHA
                     ANDALRSRLVPLNRKYPLARLMDAVRAYGSTAGRQVTFEWTLLQGVNDSVKDAQELAA
                     LIRGMRASVNLIPYNRVEGLSFEPSSPEVCKAFVARLEASGINATLRRERGGDINAAC
                     GQLRARRLAELQSGAQDSCPSSSSSDA"
     gene            complement(3076..>3457)
                     /locus_tag="PKW60_11940"
     CDS             complement(3076..>3457)
                     /locus_tag="PKW60_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00190.1"
                     /translation="ARELFCPENWGRLHDRKVRVVDAHAEPSSRLTLLVDKKFGFLRR
                     VTLDGPNWAVRESELLDRNGDVLLRVTCDEYAEMDGVRVPAVFEAAFPKQGLSLRFSL
                     AKQKMVYNPELGPGDFHQQHEGVP"
BASE COUNT          680 a         1120 c         1007 g          650 t
ORIGIN      
        1 cctgccgcta aggaggcagc cccggagccg cccgttcaaa ccgcggcggc gccagacacc
       61 gcgccgccca gagacacggc cgccccaccc gtttccatcg acaagatcga gctgccgccc
      121 gaaaaccttc ctccagcggc ggcggctcca gcccagaccc ccttttacag cgtccaattg
      181 atcgcctttt catcggcaaa tcgggccaaa gcagatgcct acgccaaaga aattcgggaa
      241 aacgccggac ttgatgtaga attggagata tcgaccgatg gaaaactcat ccgactctat
      301 gtcggacagt atgctactcg agacgacgcg gtgaaagcgt gccaggaatt gaaaaagcag
      361 gaacgcttct cccaggcttt cgtccctcag gaggcacgca agccgaagaa ctaggggacg
      421 gagcggggca tggtagcaaa agggagctga gggagcgcca tgaaagtgtc tgtaattggt
      481 ccgggtgcga tgggatgcct gttcgcggcg cggcttgcgc atgccggaat ccagacgacg
      541 ctaatcgatt acaagcctga tcgagccaaa cgattaaatg cgtccggcat ctgcgtggaa
      601 tcggacacgg agccgctgac ggccactccg ctggtcgcca cggaaattcc cgacggccaa
      661 gatctgctga tggtgttcgt gaaatcacat gtgacgccca cgctcacgct gccggcaggc
      721 gtgccggtgc tgacgcttca aaacggcctg gggaataccg aaaccctgtg ttccgccgta
      781 ggcagcgcca acgtcctggc cggcgtgacc cacgaggcgg ccatgctcaa cagcgaaggg
      841 cacgtcacgc acacggctgc cgcccgtacc gtgtttggct cctggacttc ctgtccgacg
      901 gatgccgcgg aaaaagcgat ggatgctgcc gggttccagt gtgacgtcac agcggcgccg
      961 ggccagacac tctgggaaaa agtggctgcg tccagcgcga tctatcctct gactgccatg
     1021 ctcaacatcc acaacggcgc tttgttggag gtccccgagg tgcggcagct gatgcgcgac
     1081 ctggtcgtcg aaagtgtcaa agtcgcctct acggagggat accggtttcc gtacagtctc
     1141 gtggaagagg ccgaggcttc ctgccagaaa ttcccgcagt gcatctcccc catgctgcag
     1201 gatatccggg caggccggcg cacagaaatc gagagccttt gcggcgagat cgttcgcaag
     1261 gcgcaggtag cggcgttgcc cgtaccgcgg acgcgggtca tttatcagct gatccggggt
     1321 atggaatccc gttgagcccg ccggaattaa gctacgacag cccggagacg ttcacattcc
     1381 ggcagcggat gctgctgaca ctgggctgtc ctcttcttgc tggcgcgtac aaggccttgt
     1441 gcgtgacatg ccgccaggag cggcggggcg aagagctctt tcagcgttcc ctcgcctccg
     1501 ggcagcatgt ctttgtagcc ttctggcacg aaacccttgg cctggctgcc tggctgcacc
     1561 gcaacacggg gttccatacg ctgaccagct acagtttcga cggtgaagtg gcggcacgcg
     1621 tcgttcgttc ctttggcctc tacgccgtgc ggggttcatc ttcccggggg ggctacgaag
     1681 ccctcaggca gatgcagcgc gcggtggata aagtgcccgc tgtcggtttc actctggatg
     1741 gacccacggg gccgcgcaga gtggctaagc caggggcagc catcctggcg atacgcaccg
     1801 gggtgcccat tgtcccactg gcgctcgccg cgtcctcatg ctggcgcctg cattcctggg
     1861 accagttgat catccccaag ccgtttggcc ggatcctgtc catctacggc gaccccattc
     1921 tcccgccgcc caagggggcg cccaaagcca tcgagcagct ccggtctgaa gtggaaaggg
     1981 cgctcaaccg ccttcacgca tcgcttgagg aagaactcgg gcaggaatcc tgagcgccac
     2041 tctgaagctc ggcaagcctg cgggcccgca gctggccgca cgccgcgtta atgtctcccc
     2101 cccgctcgcg ccggagcgtc gcatttattc ccgacgcttc gagccgggcc acgaacgctt
     2161 tgcagacttc aggcgaggaa ggttcaaagg aaaggccttc cacacgattg tacggtatga
     2221 gattcacgga cgcccgcatc ccacgaatca gcgccgccag ttcctgcgca tccttcacgg
     2281 aatcatttac gccctgcagc aaggtccatt cgaaggtcac ctgccgtccg gccgtgctgc
     2341 catacgcgcg caccgcgtcc atcaaccgcg ccagcggata tttccggttc agcgggacca
     2401 gccgggaacg gagcgcgtcg ttggccgcgt gcagggacac gctcaaacgc acctgccact
     2461 tttccgccgc aaaccgctca atacccggca catctcccgc ggtcgagact gtgattttcc
     2521 gggcgccgat attcaagccg tctttccgca tcagcatgcg gatcgccttg gccacggcat
     2581 cgtaattgcg aaacggctcg cccatgccca taaacacgat gtttggcgtg cggcccccca
     2641 tatcgcggcc gcgcagcaga tgcagggcct gctccacaat ctcgcccggg ctcaggctgc
     2701 gtacatagcc cgacaggccg gtggcgcaga acacgcaccg gaccgcacaa cccacctgcg
     2761 atgaaagaca cagcgtcagc cgctcaccct cgggaatcgc caccgcttcg accgttgtcc
     2821 catcgtgaag ctcgaagagc tgctttcgag tcgctgctcc ggcttcgctc gaggccaggg
     2881 tttgcagggg gcggatctga ggcaaagtga tgcgttcgcg caattcgtcg cgcatgcttt
     2941 tcgacaggtc ggtcattttg ttcagatcga agacttgttt cgcgtggacc cagcgaaaaa
     3001 gctgcctcgc ctgatatact ttgaggtcga gtgcttccgc cagttcatcc ggcagaaggt
     3061 cggtaatgtc tatcttcacg gtacgccctc atgttgttga tgaaaatccc ccggccccag
     3121 ctccgggttg tacaccattt tctgtttcgc cagggaaaac cgcagggata atccctgttt
     3181 tggaaaagcg gcttcgaaca ccgcgggcac gcgcacgccg tccatctccg catactcgtc
     3241 gcaggtgacc cgcagcagca catcgccgtt tctatccagc agttcacttt cccgcactgc
     3301 ccaattgggg ccatccagcg tgactcggcg taaaaaaccg aattttttgt ccacaagcag
     3361 tgtcagccgg ctcgagggtt ccgcatgagc gtcaacgacc ctgaccttcc gatcgtgcag
     3421 tctcccccaa ttctccggac aaaacagttc cctcgca
//
LOCUS       DAOPBQ010000404         3446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_21982_length_3446_cov_3.694191, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000404 DAOPBQ010000000
VERSION     DAOPBQ010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21982_length_3446_cov_3.694191"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PKW60_11945"
     CDS             <1..235
                     /locus_tag="PKW60_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00191.1"
                     /translation="FNREVLYGARYKLSELWAFAFEHRYDLERGEVYQQEYEVRKRFK
                     CFDWSLKVRDRTQGWDFNVAISLSAFPGTKVKF"
     gene            299..1480
                     /locus_tag="PKW60_11950"
     CDS             299..1480
                     /locus_tag="PKW60_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HPK00192.1"
                     /translation="MSSAITRIIESILPEIVELRHAFHQHPEIRFQESWTAERISGVL
                     TQWGISHQTGLARGTGIVASITGKPGKTIALRADIDGLEVEEATGLPYASKVPGRMHA
                     CGHDGHMANLLGVARVLNEFREALQGTVRFLFQPGEEQEGGARYMIADGALDGADAIF
                     GLHGSPLYPAGTIAVKSGPLMASADFFRIRVQGKGCHGADPASAIDPVIIAAHIATAL
                     QTVVSREVDPRRPAVVTIAHIEAGTTTNIIPDTALLEGTIRSVDPATDALLRAAIERI
                     AGHTAQAFRASASVRFGGNPYPPVVNDAAMTELLLDSASRTIGTKNIIALKEPQMIAE
                     DFACYLEQVPGAFFFLGMANAPDEPYPPLHSAQYDFNDRALRTGMAVMTDLALRFLSK
                     T"
     gene            complement(1528..2787)
                     /gene="aroA"
                     /locus_tag="PKW60_11955"
     CDS             complement(1528..2787)
                     /gene="aroA"
                     /locus_tag="PKW60_11955"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPK00193.1"
                     /translation="MTTPKIEIVPRRNLNAEVTIPGSKSCTARALVMAALTPGISTLS
                     GAAQGDDAGYMLEGIRALGAEVARVNDAVTVSVRRLTPSPTPLFLGNSGTAVRFLAAV
                     CAGVPGTTVLDGTPRMRQRPIGDLAEALREWGVTVETVGGFPPISITSSGSFGGTTHI
                     QGGASSQYLSGLLMTAPQADREAIILVQGELVSKPYVDLTIAMMAERGVTAENQDYRA
                     FRVAHGQRYAPGDYVIEADASGASYFFAAAAIAGGRVRVRNLGASSRQGDAGFPAVLQ
                     QMGCRVRSGPDWFEVEGTDRLCGLDIDLNAMPDMAQTLAVTALFAQGATTIRNVSNLR
                     LKECDRLDALARELRKLGAAVTEFDDGLHIIPQKLHAAEIDTYDDHRMAMSFALAGLR
                     IPGVVINDPGCVSKTFPDFFERFTALA"
     gene            complement(2784..>3446)
                     /locus_tag="PKW60_11960"
     CDS             complement(2784..>3446)
                     /locus_tag="PKW60_11960"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPK00194.1"
                     /translation="QVPAQDVMGGALFLEGAAPYFPAGTIHMAVVDPGVGTARRPLAV
                     QAGEQYFVCPDNGLLTLVLERLPLREARVITNPACMPAEISPTFHGRDIFAPAAGYLA
                     SGKAFDEMGPVAGELCRLEVPEPAQLNDTLFRGEVISIDAFWNLITNFRRESLAGRAV
                     RRVCSGTLSVERLATVFGDVAPGWPVAYFGSSGRLEIAVNCGNAALKYGIERGTAVEV
                     ET"
BASE COUNT          678 a         1108 c         1055 g          605 t
ORIGIN      
        1 gttcaaccgc gaagtcctct atggcgcgcg ctacaagctc agcgaactgt gggcgtttgc
       61 attcgagcac cgctacgacc tcgagcgagg cgaggtgtat caacaggaat acgaagtgcg
      121 taagcggttc aagtgctttg actggtcgtt gaaggtgcgc gatcgcacac agggctggga
      181 ttttaacgtc gccatcagcc tttcagcgtt ccccggcaca aaagtgaaat tctagaccgg
      241 gccggctccc cgccagatct caggctgacc ggacacggcg gattaccgag gcacaggcat
      301 gagcagcgca atcaccagaa tcatcgaatc gatcctgccg gaaatcgtgg aactccggca
      361 tgcattccac cagcaccccg agattcgatt tcaggagtcg tggacagcgg aacgcatttc
      421 cggtgtattg acccaatggg gcatttcgca ccagaccggc ctggctcggg gcacgggcat
      481 tgtcgcgtca atcaccggca agccgggtaa aaccatcgcg ctgcgcgcgg acatcgatgg
      541 gctggaggtg gaggaagcca ccggcctgcc ctacgcatca aaagtgcccg gccggatgca
      601 tgcctgcggg catgacggcc acatggcaaa tctgctgggg gtggcccgtg tgctgaacga
      661 attccgcgaa gctctccagg ggaccgtacg gttcctgttt caacccggcg aagaacagga
      721 gggtggagcc cgctacatga tcgccgacgg cgcactggac ggcgcggatg ccatcttcgg
      781 gctgcacggt tcgcctctgt atcccgccgg gaccatcgcc gtgaagagcg ggcctctgat
      841 ggccagcgcc gacttttttc ggattcgggt tcagggaaag ggctgccatg gcgctgaccc
      901 ggcttcggcc atcgatcccg taatcatcgc ggctcacatc gccacggccc tgcagacggt
      961 ggtcagccgg gaggtcgatc ccagacgccc tgccgtcgtc acgatcgcgc acatcgaagc
     1021 cggcacaacc accaacatca tccccgacac cgcattactt gaaggcacca ttcgcagcgt
     1081 tgaccccgca acggatgccc ttttgcgcgc ggccatcgag cgcatcgcgg gccacacggc
     1141 ccaggctttc cgcgcatccg cctcggtccg cttcggcggg aatccttacc cgcccgtggt
     1201 caatgacgct gccatgacgg agttgctgct cgacagcgct tcacggacca tcggaacgaa
     1261 aaacattatt gcgctcaaag agccgcagat gattgcggag gactttgcct gctatctgga
     1321 gcaggtcccc ggtgcgtttt tcttcctcgg catggcgaat gctccggacg agccgtaccc
     1381 gccgctgcac agcgcccagt atgatttcaa cgacagggcc ctccgcacgg gcatggccgt
     1441 catgacagat ctcgcgctcc gattcctgtc gaaaacgtga cgaacccggg ttggcagggt
     1501 ccgcagcgct cgagcttgcc caattcctca cgccagggcc gtaaatcgtt caaagaaatc
     1561 cgggaacgtc ttcgagacgc aaccggggtc gttaatgacc accccaggaa tgcgcagccc
     1621 cgcgagagcg aagctcatgg ccatgcggtg atcgtcgtag gtgtcgatct cagccgcgtg
     1681 aagcttctga ggaatgatat ggagcccgtc gtcgaattcc gtcacggcgg cacccagctt
     1741 gcgcaattcg cgagccagcg cgtcgaggcg gtcgcattct ttaagccgca ggttgctcac
     1801 gttccgaatc gtggtcgctc cttgcgcgaa gagggccgtc accgcaagcg tctgggccat
     1861 gtccggcatc gcgttgagat cgatatccag gccgcacagg cggtccgtgc cttcgacttc
     1921 gaaccagtcg ggacccgacc gaacccggca ccccatctgc tgaaggactg cgggaaatcc
     1981 ggcatctccc tggcgggatg atgcaccgag gttgcgtacc cgtacacgtc cgcccgcgat
     2041 cgcggcagcc gcgaaaaaat acgaggcgcc cgaagcatcc gcttcgatca catagtctcc
     2101 cggagcgtag cgctggccgt gcgcgacgcg aaacgcccgg tagtcctgat tctcggcagt
     2161 gacgccccgc tcggccatca tggcaatcgt aagatcaaca tagggttttg agaccagctc
     2221 accctggacc aggatgatgg cctcccgatc ggcctgggga gcagtcatca gcaggccgct
     2281 caaatactgg ctgctagcgc cgccctggat gtgtgtcgtg cccccaaatg agcccgaaga
     2341 cgtgatgctg atgggcggga atccgccaac ggtttcaacg gtcacgcccc attcgcggag
     2401 cgcttcagcg agatcgccga taggccgctg ccgcatgcgg ggcgtgccgt cgaggacggt
     2461 tgtacccggg acaccagcgc agacggccgc gagaaaccgc actgccgtgc ccgaatttcc
     2521 gagaaagagc ggcgtcggag aaggggtcag gcggcgcacg cttaccgtga cggcatcgtt
     2581 tacccgggcc acctcggcgc ccagggcgcg gatgccttcg agcatatatc cagcgtcatc
     2641 gccctgggcc gcgccggaca acgttgaaat ccccggagtg agcgccgcca tgacgagagc
     2701 cctggctgtg cagcttttcg accctgggat cgtcacctca gcattcaaat tccggcgcgg
     2761 gacgatttca atttttgggg tggtcatgtt tcaacctcaa cggcggttcc ccgttctatc
     2821 ccgtatttca gcgcggcatt gccgcaattg acggcaatct cgaggcgtcc gctgctgcca
     2881 aaatacgcta cgggccagcc tggggcaacg tcgccaaaaa cggtcgcgag ccgttcgaca
     2941 gacagtgtac cggagcagac tctccgtacc gcccgcccgg caagcgattc ccggcggaaa
     3001 ttcgtgatga ggttccagaa cgcatcgata gaaatcactt cgccgcgaaa cagcgtgtcg
     3061 ttgagctggg ctggttcggg cacctcgagg cggcagagtt cacccgcgac cgggcccatc
     3121 tcgtcaaacg ccttgccgga ggccaggtat cccgcggccg gggcaaaaat atcgcgcccg
     3181 tgaaacgtgg gagagatttc cgcgggcatg caggcgggat tcgtgatgac gcgcgcttcg
     3241 cgcaatggaa gacgctccag caccagggtc agcagcccgt tatccgggca gacgaagtac
     3301 tgctctcccg cctggacagc gaggggccgt cgtgccgtgc cgacccccgg gtcaaccacc
     3361 gccatatgga tggtgcctgc cggaaagtac ggcgcggctc cctccagaaa cagggcgccc
     3421 cccatgacat cctgtgccgg cacctg
//
LOCUS       DAOPBQ010000405         3442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22013_length_3442_cov_5.315028, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000405 DAOPBQ010000000
VERSION     DAOPBQ010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22013_length_3442_cov_5.315028"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PKW60_11965"
     CDS             complement(<1..681)
                     /locus_tag="PKW60_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPK00195.1"
                     /translation="MPSKADNAQEPGRKVKRVRKAPAKPAAKTAPSDAAEKAIELEES
                     TPPRKTFAQRVTAIRDTSAPRGQGFWNLASVRRRDITVFLRQLVMLLEAGTPLLKSLK
                     TLANRLEGRGIRNMVHDLADQVENGNPLWQAFERHPHEYFDTVFVALVKASEASGTLV
                     DTLRRQTEYYERREMLRRRVKTALVYPAILVVLCGAVLFVMAKFVVPAFQEIFEGALN
                     VSMPAFTRG"
     gene            complement(713..1723)
                     /locus_tag="PKW60_11970"
     CDS             complement(713..1723)
                     /locus_tag="PKW60_11970"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HPK00196.1"
                     /translation="MNMARRDYTQQGGALIIALGMLAVFTALGTAYLSYMVLEQQQSQ
                     DYLARIHAQQLADRAIWAAAGEIQAALANGEAPPLGAQEVQLLPVYRAVIEGTPEAWS
                     RRDGWVQLSIADESAKLNINHASPRVLQAMLRVDGDQARTIRGQVPRPGEAPGPEHRW
                     FTSIDDLAERGLLSSEALAQVEPQRGAFLTTCSVDDNLAPKAYLNVNTAPKEVLAAVF
                     NPEAADRIVMARAQAPITDFEGLVRAAQADPATFNLELPADGSTPPELAFQSRSFRIA
                     CEAKARKEGESLATCRVEAVVVLSDTGVAKITYWKEMPAALADEEAPEPGSDTQQAVA
                     ES"
     gene            complement(1734..2360)
                     /locus_tag="PKW60_11975"
     CDS             complement(1734..2360)
                     /locus_tag="PKW60_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK00197.1"
                     /translation="MMHLRSTRGFTLVELVVVIAILGILSTMGVVMFGRLTNAWTLTR
                     NRTELDATAEYALKQIRQDLTDLVSPALTGAPLQGTDASVPDAAFPGANAASDTLIIP
                     LRGKTSAGSETLAAVKYAVQDMGGARVLTRTQTGLYGGTQDNLAAIKVADGVAQFNVQ
                     FLGPEGQWRDAWETAATPAAVRVSLVVANPDRPERQVARETVFAVRAN"
     gene            complement(2357..2977)
                     /locus_tag="PKW60_11980"
     CDS             complement(2357..2977)
                     /locus_tag="PKW60_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPK00198.1"
                     /translation="MPHHVTVRGPASDIAAVVGMWDTPSEKGDQSVLPTHRDRPGQRH
                     RTRREGFTLVEVLVALAVLSAATWIILSLFTSSLKLEKNARSTRVAAELARDRLADIL
                     ARPAAYAWPAEDAIAGEAGAPLTLKDPPEGGAFALPAVLPASPLAQRAEKVFYERFGW
                     EAIVKRPAEGRGFYEVLVVVRWEEAKRPKQVVLTGSIPVAMTKEAS"
BASE COUNT          570 a         1108 c         1088 g          676 t
ORIGIN      
        1 cccgcgggtg aacgccggca tgctcacgtt cagcgcgcct tcaaaaattt cctggaacgc
       61 gggcacgacg aatttggcca tcacaaacag caccgccccg cacagaacca ccaggatcgc
      121 cggatagacc agcgcggttt taacccggcg ccgcagcatt tcccggcgct cgtaatattc
      181 ggtctgccgg cgcagggtat cgaccagcgt accgctcgct tcgctggctt tcaccagggc
      241 gacgaacacg gtgtcgaagt attcgtgcgg gtgccgttcg aaggcttgcc acaacgggtt
      301 gccgttttcg acctggtcgg ccaggtcgtg caccatattg cggatgcccc ggccctcaag
      361 ccggttggcc agggtcttga gcgatttcag caggggcgtg ccggcttcca gcagcatcac
      421 cagctggcga aggaacaccg tgatgtcccg ccgccggaca gaggccaggt tccagaatcc
      481 ctgcccgcgc ggcgcggagg tgtcgcggat cgccgtgact ctctgggcaa aggtcttcct
      541 gggcggcgtg ctttcttcca attcgattgc tttttcggcc gcgtcactcg gggcggtttt
      601 ggccgccggt tttgccggag ccttccgcac ccgcttcact ttccggcccg gttcttgagc
      661 attgtcagct ttggatggca ttgtaatagt ccctccattc catcgcgcgc gcttacgatt
      721 ccgccacggc ctgttgtgta tcggaacctg gctccggcgc ctcttcgtca gcgagtgcgg
      781 ccggcatttc tttccaatac gttatttttg cgacacccgt atccgataac acgacgaccg
      841 cctcgacgcg gcaggtcgcc agcgactccc cttcttttcg agctttagcc tcgcaggcga
      901 tccggaaact gcgcgattga aacgcgagtt cggggggcgt gcttccgtcc gccggcagct
      961 ccagattaaa cgttgcggga tcggcctgcg cggcccgcac cagcccttcg aaatccgtga
     1021 tcggggcctg ggcgcgcgcc atcacgatgc ggtcggcggc ctctgggttg aacacggcgg
     1081 cgagaacctc tttcggcgcc gtgttgacgt ttagatacgc tttcggggcg agattgtcgt
     1141 cgaccgagca ggtcgtcaga aaggcgcccc gctggggctc aacctgcgcg agggcctcgc
     1201 tgctgaggag gccgcgctcg gccaggtcgt caatgctggt aaaccaccgg tgttccgggc
     1261 cgggggcttc accgggccgc ggcacctggc cgcggatagt gcgggcctgg tcgccgtcga
     1321 cgcgcagcat ggcttgaagc acccggggcg acgcgtggtt gatgttgagc ttggccgatt
     1381 catcggcgat gctgagctgc acccagccgt cgcggcgcga ccaggcttcg ggcgttcctt
     1441 cgataaccgc gcggtatacg ggcaacagct gaacttcctg cgcgcccagc ggcggcgctt
     1501 cgccgttcgc gagcgccgct tgaatctcgc cggcagcggc ccagatcgcc cggtcggcca
     1561 actgctgcgc atggattctg gcgagataat cctgcgattg ctgctgttcg aggaccatgt
     1621 agctcaggta ggccgtgccg agcgcggtga atactgccag catccccagt gcgatgatga
     1681 gggcgccgcc ctgctgcgtg tagtctcgtc tggccatgtt catagataac cccttaattt
     1741 gcccggacgg cgaacacggt ttcgcgggcg acttgccgct ccggccggtc aggatttgcc
     1801 actaccaggg atacccgaac cgctgcgggg gtcgccgcgg tttcccaggc atcccgccat
     1861 tggccctctg gcccgaggaa ctgcacattg aactgcgcca caccgtcagc caccttgatg
     1921 gccgccaggt tatcctgcgt cccgccgtac aggcctgtct gggtgcgcgt cagcacgcgc
     1981 gcgccgccca tgtcctgaac cgcgtatttc accgcggcca gcgtttcgga ccccgccgat
     2041 gttttcccgc gaagcgggat gatcagcgtg tcggacgctg cgtttgcacc gggaaacgcc
     2101 gcatcgggca cagaggcgtc cgtgccctgc aggggggcgc cggtgagcgc gggcgatacc
     2161 aggtcggtca gatcctgccg gatctgtttc agggcgtact cggctgtggc atccagttcg
     2221 gtgcggttcc gggtcaaggt ccaggcattg gtcaggcgcc cgaacatcac gacgcccatg
     2281 gtcgataaaa tgcccaggat cgcaatgacg acgaccaact cgacgagggt aaacccccgg
     2341 gtgctgcgca ggtgcatcat gaggcctcct tcgtcatcgc caccggaatg ctgccggtca
     2401 gcacgacttg tttcgggcgc ttcgcttcct cccagcgcac gacgaccagc acctcataaa
     2461 atccgcggcc ctcggcgggg cgtttcacga tggcctccca gccgaagcgt tcgtaaaaga
     2521 ccttctccgc gcgttgtgcg agcgggctgg ctggcagcac agcaggcagg gcaaacgcgc
     2581 ctccttccgg cggatccttt aatgtcagcg gcgcgcctgc ctcacccgcg atggcatctt
     2641 ccgcaggcca ggcgtaggcc gccggccgcg cgaggatatc tgccaaccgg tcgcgggcca
     2701 gctcagccgc cacgcgtgtg gatcgcgcat ttttttcgag cttgaggctg ctggtgaaga
     2761 gggagaggat gatccaggtg gcggcgctga ggacggccag cgccacgagc acttcgacga
     2821 gcgtaaaacc ttcccgcctg gtgcgatgcc gctggcccgg acgatcgcgg tgtgtcggta
     2881 acacagattg gtcccctttt tcgcttggtg tatcccacat gcccaccacc gccgcgatgt
     2941 ccgatgccgg cccgcgaacc gttacatggt gcggcactgc aatctcccca tctgctgtat
     3001 cttactttac ttcacgccgt agcaataagt caattgctaa tcataataat gataatacgg
     3061 taaggactat gagggttggc acatgattct tcgggtacat gcgcaatcgt cccttttttg
     3121 cgcaggaatt cccgatgagg cgcgatcaca ccgtcggcgt agtccgattt tgcggccgga
     3181 cctggtgttt gttaaagtac gtggcgcgcg gcgctgcgga aaacgcgtgt ccaggcgaac
     3241 ccgtggcggc gccccaacct cgacagacgg aaaggaagcg gcccatgcga atcagtttat
     3301 gttgcacgtt gatcatggca gtggtggcga gcctggccgg agcacagagc cttgacggcc
     3361 ggcagattca gctgacagcc ggcagccaaa cgagcctgtg ggcgcctgtg gccttgccct
     3421 gggacggacc cgcgccggcg ga
//
LOCUS       DAOPBQ010000406         3442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22015_length_3442_cov_3.280484, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000406 DAOPBQ010000000
VERSION     DAOPBQ010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22015_length_3442_cov_3.280484"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2284
                     /locus_tag="PKW60_11985"
     CDS             <1..2284
                     /locus_tag="PKW60_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00199.1"
                     /translation="EELALTLNLQVETQAEGIQLNGTLEDLRGVDRAVTVYVPIPFDG
                     DDWSWWRDMRRNAPAQSGTFMNTHQTGAGATGQCSIYPLAALTRPDAGIALATPMTEP
                     RHCRLVYAADLNLLFAAFDLGLSPDTAKFPQRATFEAWLYAFDPVWGFRAALERYYAL
                     FPACFEKRVDREGLWMAFTDISTIPDAQDFGFAFKEGTNNVPWDEEHGLLTFVYTEPM
                     TTWLPLSKEVPREAEAALTHLTSMLETDPASRRLASVTLLSSVHGADGKPLIYLHDTP
                     WCDGALFPLNADPDLPVTEAAPLNKAQSEWAVLERTLATPERDLLPDWNHYETGYEAD
                     AAVFASGQQSLRISLTEPGKAGASQVVAIEQSASAPLVLRARVKSLGLTGVPDNDCAV
                     YADLFHTDGTPLWGQCLPIPADATDFVLLERTIESEKPFKQATVHLLMRGAHTGAVWI
                     DDLFLGEAGTERNLIADPGFEKTPVPPASVDGVYIDSFVYFASTLNYRREHFVSTDFP
                     LVFDQHSRRVGILTVFSTFQFERHAAAKVHEQGKLMMANAALHGYAFPAAYLDVLGTE
                     TNWFPNGAWKPMADESMAFRRAVCRQKPYCFLLNTHYADLDLTALERYMQRCLFYAMY
                     PGFFSEDAANDCFFENPEWYEPARPLFKKYVPLVQTIGRAGWQAITHAWADGGAYIER
                     YGNPESGVIYFSLLNESADGINTVVTIDLESLGLAEKSLTVQERITEGALGSSRMGQT
                     LRLELALPPHSARLLSFTAE"
     gene            complement(2308..>3442)
                     /gene="amrB"
                     /locus_tag="PKW60_11990"
     CDS             complement(2308..>3442)
                     /gene="amrB"
                     /locus_tag="PKW60_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPK00200.1"
                     /translation="LLGPIPFFVASQLDGLNDVTDIQYLLFRQTGGRLVSEDQIREVV
                     DYLDERGFLASPRFESLHREVLESYRDRSTRPAFLAGKSYPDDPQELREFIDELYTGQ
                     DGPGPVQPQSAASGKGVRCLIVPHIDFPRGGLAYAHGYAKLASGRKPKTVLIFGVSHA
                     AAPVPFILTRKGYETPFGTLSANTEVVDALAAACAWDPFEHEIAHRSEHSIEFQVVML
                     AHLYGPDVTIVPVLCGLLSDGLAPVSNASALPDVNAFLTACQQVMRASNGEMCVIAGV
                     DLAHVGRRFGDGFDIGDEVVRAVKARDDEDLACAMNLDADGWYASVMKDSNQRRVCGL
                     NAIYASLKTLDGAAGRGELLKYMYAHDPSGGIVSFASIAFSEE"
BASE COUNT          682 a         1058 c         1039 g          663 t
ORIGIN      
        1 tgaggaactc gcgctgacac tcaatctcca ggtagagacc caagccgaag gtattcagct
       61 gaacggcaca cttgaggatc tgcgcggagt cgatcgcgcc gtcaccgtgt atgtcccgat
      121 cccgttcgat ggagacgatt ggagctggtg gcgggatatg cggcggaatg cgccggccca
      181 gagcggcaca ttcatgaaca cccatcaaac cggcgcaggc gcgaccggcc agtgttccat
      241 ctatcctctg gccgccctga cccgcccgga tgcgggcatc gcgctcgcga cgccgatgac
      301 ggagccccgc cattgccgcc tcgtctacgc tgcggatttg aacctgcttt tcgcggcatt
      361 cgacctcggt ttgtcgccgg acacggcgaa atttccacaa cgcgcgacct ttgaggcgtg
      421 gctttacgct tttgacccgg tatgggggtt ccgggcggcg ctcgaacgct attacgccct
      481 ttttccggca tgcttcgaga aacgggtgga tcgcgaaggg ctttggatgg catttaccga
      541 catcagcacc atcccggatg cgcaggactt cgggtttgcc ttcaaagagg gcacaaacaa
      601 cgtcccctgg gacgaagaac acggcctgct cacgttcgtg tataccgaac cgatgaccac
      661 gtggctgccg ctttccaaag aggtgccccg cgaggcggag gccgctctga cccacctgac
      721 ctccatgctc gagaccgacc cggcctcccg gcgtctggcc tccgtcacgc tgctttcgtc
      781 ggtgcacggc gcggacggga agccccttat ttatctgcac gacacaccgt ggtgcgacgg
      841 cgcccttttt ccattgaatg ctgatcccga tcttcccgtc accgaggcgg ctccactcaa
      901 taaagcgcag agcgaatggg cggtcctgga acgcacgctc gccacaccgg agcgggacct
      961 gcttcccgac tggaatcatt acgaaacggg gtatgaggca gatgccgcgg tttttgcgtc
     1021 gggacagcag tcacttcgaa tttccctgac cgaacccggc aaagccggcg cctcccaggt
     1081 ggtcgcgata gagcagtctg cgtccgcccc gctggtgctg cgtgcacgtg tcaaatcgct
     1141 cgggctcact ggcgttccag acaacgactg cgccgtctac gcggatctgt tccatacgga
     1201 cggcacgccg ttgtgggggc agtgcctgcc cattcccgcg gacgccacag acttcgtgct
     1261 gctggaacga accatcgaat ccgaaaaacc gttcaagcag gccaccgtgc atctccttat
     1321 gcgaggggcg catacgggcg cggtctggat tgacgacctg tttctgggag aagcaggcac
     1381 ggagcgcaat ctaatcgccg atccgggctt cgagaagact ccagtgccgc cagcgagcgt
     1441 ggatggggtc tacatcgact cgtttgtgta ttttgcctcg acgctgaact accgccggga
     1501 acattttgtc tccacagatt ttccgctggt ttttgatcag cattcgcggc gcgtgggcat
     1561 cctgaccgtg ttctcgacat tccaattcga gcgccatgca gcagcgaagg tacatgagca
     1621 gggaaaactc atgatggcga atgccgcgct gcatggatac gcctttccgg ccgcgtatct
     1681 cgacgtgctg ggcacggaaa ccaactggtt tcccaatggc gcatggaagc ccatggccga
     1741 cgaatccatg gccttccgcc gcgccgtctg caggcagaaa ccgtattgtt tcctgctgaa
     1801 tacccattac gccgatctcg atctgaccgc gctcgagcgc tacatgcagc gttgcctttt
     1861 ctatgccatg tatccgggct tcttcagcga agacgccgcg aacgactgtt ttttcgaaaa
     1921 tccagagtgg tacgaacccg cccgccccct tttcaaaaag tacgtcccgc tggtccagac
     1981 gatcgggcgg gcaggctggc aggcgatcac gcacgcctgg gcggacggcg gcgcctatat
     2041 cgagcgctat ggtaatccgg agtcgggcgt tatctacttt tcgctgctca atgaatcagc
     2101 agacgggatc aacacggtcg tgacgatcga cctggagtcg ctcggattag cagaaaaatc
     2161 gttgacagtt caagagcgga tcacggaggg agcgctggga tcgtccagga tgggccaaac
     2221 gctgcggttg gagctggctc ttccccccca ttcggcccgg ctgctgtcgt tcacggctga
     2281 ataaacggcg gaaaccgcgc aacgagccta ctcttccgaa aatgcaatgc tcgcgaagga
     2341 gacgatcccg ccagaaggat cgtgggcgta catgtatttg aggagttcgc cacggccggc
     2401 cgcgccatcc agcgttttca gcgaggcgta gatggcgttg aggccacaca cgcgccgctg
     2461 gttgctgtct ttcatgaccg aggcgtacca tccatcggcg tccaggttca tcgcgcaggc
     2521 caggtcttcg tcatcgcggg ctttcacggc ccgcaccact tcatcgccga tgtcgaagcc
     2581 gtcgccgaaa cggcgcccga cgtgcgccag gtccacgccg gcaataacgc acatctcgcc
     2641 gttcgaagcc cgcatgacct gttggcaggc tgttagaaat gcgttgacat ccggcagagc
     2701 ggacgcattc gagaccggtg cgagcccatc ggagagcaaa ccacagagga caggcacgat
     2761 ggtgacgtcc ggcccataca gatgggcgag catcacaacc tgaaactcga tggaatgctc
     2821 agagcggtgg gcaatttcat gttcaaacgg gtcccaggcg caggccgcgg cgagcgcatc
     2881 cacgacctcg gtgttggcgc tgagcgtgcc gaatggcgtt tcgtagcctt tgcgggtgag
     2941 aatgaacggc acgggcgcgg ctgcgtggga aactccgaaa atcagcacag ttttgggctt
     3001 ccggccggag gcaagctttg catacccgtg ggcgtacgcc agcccgcctc tgggaaaatc
     3061 gatatgcggc acgatcaggc agcgaacgcc ttttccggat gccgcgctct ggggctggac
     3121 gggtccgggg ccatcctggc ccgtatacag ttcatcgata aactcgcgta gctcctgagg
     3181 atcgtccggg taggatttac ccgccagaaa agcaggccgc gtgcttcggt cccggtacga
     3241 ttcgagcact tcccggtgca gagactcaaa tcgcggagac gcgaggaacc cgcgctcatc
     3301 aagatagtcg accacctcac gaatctggtc ttcagagacc aggcggccgc cggtctgccg
     3361 gaaaaggagg tattggatat ccgtgacgtc gttcagcccg tccagctggc tggccacgaa
     3421 aaagggaatg gggcccagca ga
//
LOCUS       DAOPBQ010000407         3426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22138_length_3426_cov_3.506081, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000407 DAOPBQ010000000
VERSION     DAOPBQ010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22138_length_3426_cov_3.506081"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1393
                     /locus_tag="PKW60_11995"
                     /pseudo
     CDS             1..1393
                     /locus_tag="PKW60_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773227.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
     assembly_gap    1394..1403
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1599..>3426)
                     /locus_tag="PKW60_12000"
     CDS             complement(1599..>3426)
                     /locus_tag="PKW60_12000"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HPK00201.1"
                     /translation="VFRTHGQKAILDALSYNGYPEDTVEANLGDEISLELELSEDEQN
                     QRFQKAMQERGFDPLDLLSTDDIPLVLNQPESVQWDLVAALPALDDNPVQFYEAAIFR
                     YQLWYEALAKRTRAAVEANPGKRVLAGANFSPHMNVWPDTRQWIGPFEAEAMTMTWTE
                     DWWWQVPEISPQVYGFLLDGLRLAGSYHGAPMQFYIMPFKGSSPENFTRMHGLALSHG
                     AKIINHFHTEDQVLTTWDYIDAAESPPTLRAIHDVIRDTGAVEDRVYSAMPRKAGIAI
                     LLSWAADTWDTQDLGGAGNLYSAEYNVNNDERKALWLALRHAQYPVDLITDKDVAAGK
                     LQDYRVLYVVGAEMLSQAVEPLRQWVEDGGTLYATAGGGLLDQYHRTNDALHEVYGIL
                     SNTLERPVRHIRPRNTLPKATPLDTVRIKDAKGFPDAELPALLYRETVEPGTAAVIGR
                     YDADNAPAIVHNRFGKGQAIYSGALAGIAYITPAFTKDSDTLPTDFPADRRSFVAAVP
                     QLAGLTPPVVTSHPLVEAQYMHGDNGAVLILTNWSPEPIEKLQVSFPGLRDVKRVQSL
                     RSAGHFMGALSKQDAGVLALTKTQNGPSVSLSLALVDFLYIN"
BASE COUNT          636 a         1024 c         1133 g          623 t
ORIGIN      
        1 gttccggaaa aagcgcagcg atgacgcgta cacgcccgga gcacgtgcgg gagcccggcc
       61 caaccgggcg accatcgtct acaccaacct gctgcgccag gccttccccg gcctgccggt
      121 ggtgattgga gggatcgaag cgtcgttgcg gcgggcgtct cattatgatt tctggaccga
      181 ccgcgtacgc cgctcgattc ttctggacag caaagcggac ctcttgattt atggcatggg
      241 cgagcgggcc atcctggaag cagcccggcg gctggaggcc gccgcaaacg ccgccgcgca
      301 cccggcggag tcgttgcggg gcatcccggg gaccacgttt gcggcatcgt ctctggccgg
      361 ggtgttggaa ctgccgaacg ctccggatcc ggaggccctc gtgcatttgc cctcgcacga
      421 ggcgatagta cgcgagccgc cggaactgat ggcggccacg cttgcgcttg agctgcaggt
      481 tcaccagggc acgcagtggg cggtgcagga caacggcggg cgccttgtcg tattcactcc
      541 accggctgaa ccgctcaaca cgaacgagct ggacgcactg tatcgtctgc cgttcacacg
      601 gagggcccac ccggcatata aggaagaggt gccggcggcc ggcatgatcc agttcagcgt
      661 cacatcgcac cgcggctgcg cgggcgggtg cacgttctgt tcgatagccc tgcatcaggg
      721 gcgccggatc cggtcgcgga gtgcggactc actggagcag gaggtccagg agcttacagc
      781 gcatccggac tggcggggaa gcatcagcga cgtaggcggc cccacggcca acatgtgggg
      841 agcgcgttgc cgtgcaaatc cgggcacatg ccagcgggcg gactgcctca cgcccgagct
      901 ttgcccccac ttcagcctgg acgaggcgga aaacgtacgg ctgttgcggc gcctgcgcgg
      961 cgtcaacggc gtggaccacc tgcgcgtggc cagcgggata cgctacgacc tcggccacga
     1021 caggtcttcc ctccgaacgc tgatcaccga attcgtgggc ggtcaactga aggtcgcacc
     1081 cgaacacagt tcggacaagg tgctgcggct catgcggaaa ccgtcttttc ggcaatttga
     1141 ggcatttttg ggtctgtttg aacgggaatc gcagcgggca ggcaaagagc agtacgtggt
     1201 cccttatttg atcagcgcat ttcccggctg cacggacgat gacatgcgcg ccctcgcgga
     1261 ctggcttcgc gaacggggct ggaaaccgcg ccaggtgcag tgtttcattc cgacgccggg
     1321 tacggtggct acggcgatgt actatgccgg catcgatccg cagctcgacc ctgccgcgct
     1381 gttccggcag ctcnnnnnnn nnnagcccgg agcgaccagg cgcggctccg ccagcatcat
     1441 atcctggcac cgaaccgcgg cgtgccggaa aagaaccggc gcagatttcg caaaccacgg
     1501 gacggcgcgc cctgacgcgc cgggctcgcc gccaggccag ggaccggcgg gagcaaggtg
     1561 aaattaccgt ctcatgacct ggccgggcgc ccacgcattc aattgatgta gagaaaatcg
     1621 accagggcga gggaaagcga aacggacggg ccgttttgcg tcttcgtcaa cgccagcacg
     1681 cccgcatcct gtttgcttag agcgcccatg aaatgcccgg cggagcgcag gctctgcact
     1741 cgcttcacgt cgcgcaaccc tggaaacgag acctgcagtt tttcaatcgg ttcgggcgac
     1801 cagttcgtca gaatgagcac ggctccgttg tcgccgtgca tatactgggc ttccaccagc
     1861 ggatgggacg tgacgaccgg cggcgtcagg ccggccagtt gcgggacggc cgcaacaaat
     1921 gagcggcgat ctgcggggaa atcggtggga agcgtgtcag aatccttcgt gaatgcgggt
     1981 gtgatgtagg cgattcccgc cagtgcgccg gaatagatcg cctggccctt gccgaaccgg
     2041 ttatggacaa tcgccggggc attgtctgcg tcatagcggc cgatcacggc ggccgtgccg
     2101 ggttcaacgg tttcccgata gagcaatgcg ggcaactcgg cgtccgggaa gccttttgcg
     2161 tccttgatcc gcacggtatc gagcggcgtg gctttcggga gggtgtttcg gggccgaatg
     2221 tggcgcacgg gacgttcaag ggtgttggag agaatgccgt acacctcgtg cagggcatcg
     2281 ttggtgcgat ggtactggtc cagcagcccg cccccggcag tcgcgtagag cgtaccgcca
     2341 tcctcgaccc attggcgcag aggttcgacg gcctgtgaga gcatttctgc accaacaaca
     2401 tagagcaccc ggtaatcctg aagcttcccc gcggcaacat ccttgtcggt aatgaggtca
     2461 accggatact gggcatggcg caaggcgagc cacagggcct tgcgctcatc gttgttgacg
     2521 ttgtattcgg cggagtaaag attgccggcg cctcccagat cctgcgtatc ccaggtatca
     2581 gcggcccagg acaggagaat cgcaatgccg gccttgcgag gcatggcgga atacacgcga
     2641 tcttcgacgg cgccggtatc gcggatgaca tcgtggatgg cccgcagcgt gggcgggctc
     2701 tcggctgcat cgatgtaatc ccaggtcgtc aggacctgat cctcggtgtg gaaatggttg
     2761 atgatttttg cgccatgcga cagggccaga ccgtgcattc gggtgaaatt ttcgggggaa
     2821 ctgcctttga aaggcatgat atagaattgc atgggcgcac cgtggtaaga gcctgccagg
     2881 cggaggccat cgagcagaaa tccatagacc tggggcgaaa tttccggcac ctgccaccac
     2941 caatcctccg tccaggtcat ggtcatggct tcggcctcga aaggcccgat ccactggcgg
     3001 gtatcgggcc agacgttcat gtgcgggctg aaattggcgc ccgcgagcac gcgtttgccg
     3061 ggattggcct cgacggccgc ccgggtacgc ttcgccaggg cctcatacca cagctggtag
     3121 cggaaaatgg cggcctcata aaactggacg gggttgtcgt cgagggccgg gagagctgcc
     3181 acgagatccc actgtactga ttcgggctgg ttgaggacca ggggtatatc atccgtgctg
     3241 agcaggtcca gcggatcgaa tccccgctct tgcatggcct tttgaaagcg ctggttctgt
     3301 tcatcctccg acagttcaag ttcgagagag atctcgtcgc cgagattggc ctcgacggta
     3361 tcttccggat agccgttgta gctcagggca tcgaggatgg ccttctggcc atgcgtgcga
     3421 aagacg
//
LOCUS       DAOPBQ010000408         3424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22160_length_3424_cov_4.955180, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000408 DAOPBQ010000000
VERSION     DAOPBQ010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22160_length_3424_cov_4.955180"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PKW60_12005"
     CDS             <1..421
                     /locus_tag="PKW60_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPK00202.1"
                     /translation="YNMMHRWIESDLLEYTAQYGAGVIVFSPLAQGLLTNKYLDPANP
                     IPEASRAADPDGFLRREAVTEEVVSRVRQLAELAGARGQSVAQLAIAWVLRDPRVTSA
                     LIGARTPGQVKDCVGALDNLSFTEDELATIDALFPAS"
     gene            complement(429..2792)
                     /locus_tag="PKW60_12010"
     CDS             complement(429..2792)
                     /locus_tag="PKW60_12010"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPK00203.1"
                     /translation="MKTTENMRLTLRECLSHSALLVALLAVLFPGTFLRGELISPADI
                     LFALPHWNEYAPAGWQSTSNSAIGDHVFFTRPNTLLCQQALKRGEWPLWNHLQLAGMP
                     LLANFQSAIFYPGHLHNLVLPIDWALTASVLLKLFLCGLLGYASARLLAFTPALARFF
                     SIAWMLGSYNLIWCYWPLTDVAIWVPPLFTGLEFVLRDQYRRGWYAMLFSGTLLLLAG
                     HPETAFTFGLGLGGYFLVRVIQQRRTGMRLAVPIAVCGLAWLMILAVCAAQLLPFFEY
                     LVHSTSELIREDAVGAETYAPGMTAAFWVPRFYGTDVEGNLRAAHINSNLIGMLYPGL
                     VIWVGGMLLFARGNGTKKERDRRNAAVALAIPAALGLMLSFYTPSLQFVHELPVMKEL
                     REWYHACFAAFAFPLVATMGLEHWFARQRRLRELLWALPPLLIGAAFVFLLLRFDRPV
                     LELTGEWSYALQESWRAAGLAALALALCAVFCFKQQSRIALLSLTLLAAGDSLLATRG
                     LRPTLPPEQSFPKTALIKRLQSFDQPCRVGVAEGYVPSGTVSNYGIEEWLGYDGLYPE
                     RVRRLQLELGPAIWNAMEPVCAIHYYLNYPEYGSVIDAAKHPYLKRVDTIDGMELYEN
                     TCAFPRAFLVPQVEVIPDFDAMFKIMRDPEFDPAQVVVTEEGPAVPNRSPDNAPPGQA
                     TVAVREPTRVLVEVDAACTCALVLADAYYPGWKVTIDGKAGEIFPAYYAFRGVLVPAG
                     RHTVEFTYFPLSFRLGLAISTAALLLGAFLAIRELRACGHQRSARIS"
     gene            complement(2848..>3424)
                     /locus_tag="PKW60_12015"
     CDS             complement(2848..>3424)
                     /locus_tag="PKW60_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK00204.1"
                     /translation="RCGSAASAPDVSIGRAEKVAPLEIKAPVAARPSTKPAKASGNPP
                     SPKPYGTKASPFEEPGAPPLPSLLLIDENETTREVLKETLRNRGFSVIAAQNAVRAMQ
                     AIAATLFDAVLLDFELRDVHGPNMLTEIHNIRPGVPVIVMTADTNPRTAQSALERGAR
                     ACLSKPFALHELLQKVEGAVGIHDNEQPVTR"
BASE COUNT          740 a         1002 c         1078 g          604 t
ORIGIN      
        1 ctacaacatg atgcaccggt ggattgaatc cgatctgctg gaatacacgg cgcaatatgg
       61 ggcgggggtg attgtattca gtccgcttgc ccaggggttg ctcaccaata agtacctgga
      121 ccccgcgaat ccgattccag aggcatcgcg cgcggccgac ccggacgggt tcctgcgccg
      181 ggaagccgtc actgaggaag tcgtgtcgcg ggtacggcag ctcgcggagc tggccggggc
      241 gcgcggccag agcgtggcgc aactcgccat cgcgtgggtt cttcgcgatc cccgggtgac
      301 gagtgcactc atcggcgcac gcacacccgg gcaggtgaag gactgcgtgg gcgccctgga
      361 taatctctcc tttacggaag atgagctggc aaccatcgac gcgctgtttc cggccagctg
      421 agccaccgtc aggaaattcg ggctgaacgt tgatgtccgc acgctcgaag ctcgcgaatg
      481 gctaaaaacg ctcccagaag cagggccgcc gtggagatgg ccagtcccag gcggaagctg
      541 agcgggaagt aggtaaactc gacggtatgc cgcccggcgg ggacgagcac tccgcgaaaa
      601 gcatagtacg cggggaagat ctcccccgct tttccgtcga tcgtcacttt ccagccggga
      661 taataggcgt cggccaggac aagcgcgcat gtgcaggctg catctacttc gaccagaacg
      721 cgtgtgggtt ctctcacggc cacggtggcc tgcccgggcg gggcattatc cggcgaacgg
      781 ttcggcactg ccggaccctc ttcggtgacg actacttgcg cagggtcgaa ttccgggtcg
      841 cgcatgattt tgaacatcgc atcaaaatcc ggaatgactt ccacctgcgg aacgagaaac
      901 gcgcggggaa aagcgcaggt attttcgtag agttccatgc cgtcgatggt atccacgcgt
      961 ttcagatatg gatgtttggc agcatcgatc acggagccgt attcgggata gttcaaataa
     1021 tagtggatag cgcataccgg ttccatggca ttccagatgg cggggcccag ttccagctga
     1081 agccgccgga cccgttcagg atacagtccg tcgtaaccca gccactcttc gatgccgtaa
     1141 ttgctcacgg ttccgctcgg gacatatcct tccgcgacgc ccacgcggca gggctgatca
     1201 aaggattgga ggcgtttgat cagggcggtt ttcgggaagg attgctcggg aggtaaggtc
     1261 gggcgcaacc cgcgggttgc cagaagagaa tcgcctgcag cgagcagggt caggctgagc
     1321 agtgcgatgc ggctctgctg tttaaaacaa aagacagcgc acagggcaag ggccaaggcc
     1381 gccagcccgg ccgcacgcca ggattcctgg agcgcgtaac tccactcgcc tgtcaattcc
     1441 agaacaggcc gatcgaagcg cagcagcaga aagacgaacg ctgcgccgat aagcaatggc
     1501 ggcaaagccc acaggagctc gcgcaggcgg cgttgccgcg cgaaccagtg ttcgagcccc
     1561 atcgtggcca cgagagggaa agcgaacgcc gcaaaacacg cgtggtacca ctcgcgcaat
     1621 tccttcatga ccgggagttc atggacaaac tgcagagaag gggtgtaaaa actcagcatc
     1681 aggcccaagg cggcgggtat agccagagct acggcggcat tacgccgatc acgctctttt
     1741 tttgttccat ttccccgcgc gaagagcaac atgcccccga cccagattac cagtccaggg
     1801 tacagcatgc cgataagatt ggagttgatg tgagcggcgc gaagatttcc ttcgacgtcc
     1861 gtgccataaa aacgcggaac ccagaacgcc gcggtcatcc ccggagcata ggtctccgcc
     1921 cccactgcgt cctcgcggat caactcgctg gtgctgtgca ctaggtattc aaaaaacggg
     1981 agcagctgcg cggcgcatac cgccaatatc atcagccacg ccaatccgca cacggcgatt
     2041 ggaactgcca ggcgcattcc cgtacggcgc tgctgaataa cccgcacaag gaaatagcct
     2101 cccaggccca ggccgaacgt gaatgccgtc tcggggtgcc cggcgagcag cagcagggtt
     2161 cctgaaaaga gcatggcgta ccagccccgc cggtactggt cgcgcagcac aaattcgagc
     2221 ccggtgaaca ggggcggcac ccaaattgcc acgtcggtca gcggccagta acaccaaatg
     2281 agattgtagc ttcctaacat ccatgcgatt gaaaaaaacc gggccagcgc tggagtgaac
     2341 gccaacaggc gcgcagatgc gtagcccagc agcccgcaca ggaagagttt cagaagcacg
     2401 ctggcggtca gtgcccagtc gattggaaga accaggttgt gcagatgccc cggatagaat
     2461 atcgcgctct ggaagttcgc caacagcggc atgcccgcaa gctggagatg attccacaga
     2521 ggccattcgc ctctcttcaa tgcctgctgg cagagcaggg tattgggacg cgtgaagaaa
     2581 acatggtcgc cgatggcgct gttcgatgtc gattgccagc ccgcgggagc gtattcgttc
     2641 caatggggca gcgcaaagag aatatccgcc ggagaaatga gctcgccgcg caggaacgtg
     2701 ccgggaaaga gcactgccag cagcgccacc agcagcgcgc tgtgagacaa acactcacgc
     2761 agagtcaggc gcatgttttc cgtggttttc atgtgatggc gaggagccgc cggagatccc
     2821 ggtgaatgcg gcgcgaccgc tccagcgtca gcgggttacc ggctgttcat tatcatgaat
     2881 ccccacggcg ccttcaactt tctgcaggag ctcgtggagg gcaaacggct tggagaggca
     2941 ggcgcgcgcg ccccgctcaa gggcggactg agcggtgcgg ggattggtgt cggcggtcat
     3001 cacgataacg ggcacgccgg gccggatgtt gtgaatctcg gtgagcatat tcgggccatg
     3061 gacgtcacgc agctcgaaat cgagcagtac cgcgtcaaac agggtggccg cgatggcctg
     3121 catggcacga accgcgtttt gggctgcgat cacggaaaat ccccggtttc gcagggtttc
     3181 tttaagcacc tcacgcgtcg tctcgttttc atcaatgaga agcagcgacg gcaacggggg
     3241 cgctccgggc tcttcaaacg gcgaggcctt tgttccatac ggtttaggag aaggaggatt
     3301 tccagacgcc ttcgccggct tggtcgacgg ccttgctgcc acaggggcct ttatttcgag
     3361 gggcgccact ttctctgcac gtccgatgct cacatccggt gcggaggcgg cgcttccgca
     3421 gcgg
//
LOCUS       DAOPBQ010000409         3404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22298_length_3404_cov_3.789191, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000409 DAOPBQ010000000
VERSION     DAOPBQ010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22298_length_3404_cov_3.789191"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>242)
                     /locus_tag="PKW60_12020"
     CDS             complement(<1..>242)
                     /locus_tag="PKW60_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HPK00205.1"
                     /translation="PAAFYRPAGGEGLVLGAYEILKTGKSGLSQTNIEDMHHAVGILK
                     YMKRPACAVMKHCNPSGAAIQVGDMPLAAVYQRARDA"
     assembly_gap    243..252
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     regulatory      complement(427..515)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(627..1724)
                     /locus_tag="PKW60_12025"
     CDS             complement(627..1724)
                     /locus_tag="PKW60_12025"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HPK00206.1"
                     /translation="MPSQTTSRRMHAGLLLRGALAVFLLVSWTGCGRNEPAPAPAPHD
                     EMPQQPESALEVPLAEHLDDTLRMNQIQVIATHNSYHAMPPAALHAQVKAVYPDAATW
                     EYDHAPLGLQLNRGIRSFELDIHHTLRGYEVFHVQDFDAASTCGDFEGALRAIRTWSK
                     AHPRHVPIITLIELKKEDVPFANTQTFPFEAADLVALDAQLLSVFSPEELITPDDVRG
                     EYPTLAEAVRAGKWPTLGQARGRCMFVLHVRGPLAEDYTRDAPSCQGRPMFVEGEGDK
                     PWDAVFVINEPELPELAHYVRQGFIVRTRADADLREGRANDTARRETALASGAQIVST
                     DFPRGEAEPGTRYQVELPGNVTARPNPVTAP"
     gene            complement(1896..>3404)
                     /locus_tag="PKW60_12030"
     CDS             complement(1896..>3404)
                     /locus_tag="PKW60_12030"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPK00207.1"
                     /translation="NAVGLGNFLRFPGKAAPYGGAFMVPYFLSLLLLGVPLMWIEWTI
                     GRHGGRYGHGSTPGMFHRLWKHPASKYLGIFGVLLPALIGIYYIYIESWSLGYAFETI
                     TGSYWGRTSHDQMAGVFTGYLGLNKGFLSFAPKSYFFFMITLGINIAVFARGIEKGIE
                     VVSKFAMPTLFAVGVILAIRVLTLSPIEGRTVGDGLARIWVIDWSTLRNPSMWIDAAG
                     QVFFSLSLGLGMVHTYASYVGKNEDVTLNALATCSTNEFVEVILGGTIAVPAALIFFG
                     PAETDAIVKGGTFHIAFHSLPVIFQQLPAGRLFGTLWFILLFLAGLTSSMAMFTPLLS
                     FLKEELRMNKRKAVVAISIGMFLLMQPVVLFMNQGVMDELDFWAGTFFLVLFAAIESV
                     VFAWIFGVKKGWEEMHKGADLQVPRLFFYIMKFATPAFAIALLGWWIYGGMWPKLMMK
                     DVQPEHVPYLWLARAIIVAGWLALAWGVGYAWRTHPKFFEEERVVEPGETLP"
BASE COUNT          688 a         1025 c         1063 g          618 t
ORIGIN      
        1 gcgtcgcgcg cccgctggta gactgccgcc aggggcatgt cgcccacctg gatggctgcg
       61 cccgacggat tgcagtgttt cataacggcg caggcggggc gcttcatgta tttgaggatg
      121 ccgaccgcgt ggtgcatgtc ttcgatattg gtttgagaca gcccgctttt tccggtcttg
      181 aggatctcgt acgcgccgag tacgaggcct tcgccgccgg cgggccggta aaatgcggcg
      241 ggnnnnnnnn nnattagtgc cgtagcggag atcttccacc ttggtatagg cccgccccag
      301 cagttcgatc acctccggga aattgccttc cgtgcgcgta cggtacattt gcttgagatc
      361 tttttccgcc atgggtcgct ctcctgcttc ggaaacctct aaaaacagca gcgcccgggc
      421 cactgtcttc agtgcccagg cgaacggcaa gcctaccgcg cgcgccgctt ccccgtggtc
      481 atttccacgc ccagtcgcca gttacggccg cacttgttca tttctactcc ccagcgcttc
      541 gcgccgcaag agcgccggct ccgcacccag tgccagcggc gtcaatctgc cacagccgcc
      601 gcggggaagt caatcggtgc agcccgtcag ggcgcggtca cgggattggg gcgggcagtg
      661 acgttgccgg gaagctcgac ctggtacctg gtgcccggtt cggcttcgcc gcgcgggaaa
      721 tcggtgctga cgatctgcgc accgctggcc agtgcggttt cacggcgggc ggtatcgttt
      781 gcgcggcctt cccgcagatc ggcatcggcc cgcgtccgga caatgaaacc ctgccggaca
      841 tagtgcgcga gctctggcag ctcgggttcg tttatcacaa aaacggcgtc ccagggtttg
      901 tctccctcgc cttcgacgaa catggggcgg ccctggcaag acggggcatc ccgggtgtaa
      961 tcctcagcca gagggccgcg gacatgcaat acgaacatgc atcggccccg cgcctgcccc
     1021 agcgtgggcc acttgccggc gcgcaccgcc tccgccagcg tgggatattc gccgcggacg
     1081 tcgtcggggg tgatcagctc ttccggggag aataccgaaa gcagttgcgc gtcgagggcg
     1141 accaagtccg ctgcttcgaa cgggaaggtc tgggtgttcg cgaaggggac atcctctttc
     1201 ttcagctcaa tgagggtgat gatgggcacg tggcggggat gggctttcga ccaggtccga
     1261 atcgcacgca gggcgccttc aaaatccccg caggtggagg ccgcgtcgaa atcctgaacg
     1321 tgaaaaacct cgtagcctcg aagggtatgg tggatatcca gctcaaagct tcgaatgccg
     1381 cggttgagct gcagaccgag cggcgcgtga tcgtattccc aggtggctgc gtcgggatag
     1441 accgccttga cctgtgcgtg cagggcggcg ggcggcatgg cgtgatagct gttgtgcgtt
     1501 gcgatgacct ggatttggtt catgcgcagc gtgtcatcga gatgctcggc cagcggcact
     1561 tcgagggcag attcgggttg ctgcggcatt tcgtcgtgcg gcgccggcgc gggcgcgggt
     1621 tcgttccgcc cgcatcccgt ccacgagacg agaagaaaca cggccagggc gccgcgaagc
     1681 aagaggccgg catgcatcct gcgcgaggtg gtttggcttg gcatgtggcg tactccttca
     1741 gacgcgagag ccccacaggc ctggcgcgac ggagcgcccg ggaaaccggc tcccgcttca
     1801 gcgcgacttt ttacggaaaa tgcggacgaa gcagaagaca tttatcgcgg tgatgacgga
     1861 ccagacgagg agcatgtaag cccaggaacc ggcggtcatg gcagggtctc cccgggttca
     1921 actactcttt cttcctcaaa aaatttgggg tgggttcgcc acgcataccc aacgccccac
     1981 gcgagcgcga gccagcccgc cacgatgatc gcccgggcca gccacaggta cggcacgtgt
     2041 tccggctgga cgtccttcat catgagtttg ggccacatcc cgccataaat ccaccagccc
     2101 agcagggcaa tcgcgaacgc cggcgtggcg aatttcatga tgtagaaaaa gagccggggc
     2161 acctgcagat ccgcgccctt gtgcatctct tcccaccctt ttttcacacc aaaaatccag
     2221 gcaaagacca cggattcaat cgcggcaaag agcacgagga aaaaggtgcc ggcccagaaa
     2281 tccagctcat ccatcacgcc ctggttcata aacaacacaa ccggttgcat cagcaagaac
     2341 atgccgatac tgatggcaac aactgccttg cgtttattca tccgcagctc ttccttgaga
     2401 aaggataaca gcggcgtgaa catggccatg ctcgaggtca gccccgccag aaagagcaaa
     2461 atgaaccaga gcgtgccgaa cagccgcccg gctggaagtt gctggaaaat caccggcagt
     2521 gaatggaagg cgatgtgaaa ggtgccgccc ttcactatcg cgtcggtctc cgcgggcccg
     2581 aaaaagatca gcgcggcggg cacggcgatg gtgccgccaa ggataacctc aacgaattcg
     2641 ttggtgctgc acgtggccag ggcgttcagg gtgacgtctt cgttcttccc aacatagctc
     2701 gcgtaggtgt ggaccatgcc gagccccagg ctcaacgaga aaaagacctg cccggcggcg
     2761 tcgatccaca tcgacgggtt cctgagcgtg ctccagtcga tcacccagat gcgtgccagg
     2821 ccatcgccga ccgtgcggcc ttcgatcgga gacaaggtga ggacgcgaat ggccagaatg
     2881 acgccgaccg caaacagggt gggcatggcg aacttcgaga ccacctcgat gcctttttca
     2941 atgccccgtg cgaagacggc aatgttgatg ccgagcgtga tcatgaagaa aaaatacgat
     3001 ttcggggcga acgacaggaa acccttgttg agcccgagat agcccgtaaa cacaccggcc
     3061 atttggtcgt ggctggtgcg gccccaatac gagccggtga tggtctcaaa cgcgtagccg
     3121 agcgaccagg attcgatgta aatgtagtaa atgccaatca gtgcggggag aagcacgccg
     3181 aagatgccca gatatttgga tgcgggatgt ttccagagcc ggtgaaacat gccgggcgtg
     3241 ctgccatggc cgtagcgtcc tccgtggcgg ccgatggtcc attcgatcca catcagcggg
     3301 acgcccagca acagaagtga aaggaaatag ggcaccatga atgcgccgcc gtatggtgcg
     3361 gccttgcccg gaaagcgcag aaaattaccc agccccaccg cgtt
//
LOCUS       DAOPBQ010000410         3391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22413_length_3391_cov_3.374700, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000410 DAOPBQ010000000
VERSION     DAOPBQ010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22413_length_3391_cov_3.374700"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2532)
                     /locus_tag="PKW60_12035"
     CDS             complement(<1..2532)
                     /locus_tag="PKW60_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00208.1"
                     /translation="MEEIPCRDCYNAFDDISEESTIGEDGLMIERWLRITLILGAVAA
                     FAVPVSLAQTVDVPNASFEEGQDGPAGWTLSGGSGQWLDGEASSGSRAIQVTGTGNDS
                     NFWRSGAIALEPSSVYMVTFDARNWGGSGGTAITGPSFCNRDLGVLTGEWKSYSSVFF
                     TPDAPGEGGLRFGQWQHNGSMAFDNVAIFAVHPLYVEQEGMRLGEGERIDGLSYIFSA
                     PHYSASRNQSRPLIRQQCHFNTNRWTFGAGSELVYRHQLAGRQQTGASVEVSLSYYSA
                     GRLAAEASRDGNDWTPIGEAAADGGASFTLPDNLFPASEIWIRLRGVPGDAAEGRAGS
                     CAFTLNDYTYRATLDGTPVTLQGRTRFVAVTAATPGINVTVDSLGDVLPGGANTARLL
                     VDNRADTPVFVTPTVSLTGEGGRLEQDCPPVELPAGTSVLEIPYEVPGSGAFALEVAL
                     DPGPVFAFHADFHVPHLFETAYGERVPGSTADVALWRASSGWKIAQTRPAPSRQADAV
                     ELRLARNEAEAVQFVITPQRPLTGLTVTAGDLAGPAGAVLPASAVEVLRVRYVDVVYP
                     TDETGVAAPWPDPLPPFRGPIDVAAGMNQPLWIRVNAPKDQPAGIYRGSLRVTADGFM
                     AETPLEVEVYDFELPDRMTCVSAFGFSPGLVFEYQKLSDPDQRREVMDKYLVNFSRHH
                     ISPYDPAPLDGIGLTWTGLPKWRGGEVDKETKKSGHASRFLNDPYTDKSISLDYADRL
                     PIPEKGLRVRFWYKTKEPGQRFLTSIRHFDAAGKWMSGRNLDTITTGDGSWQSFECTI
                     SIFPDEARFCAFAVFPTLWSNSGDTTGAIWLDEFVVEDAGTGQALT"
     gene            2661..>3391
                     /locus_tag="PKW60_12040"
     CDS             2661..>3391
                     /locus_tag="PKW60_12040"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HPK00209.1"
                     /translation="MYTALLTLSFLCTYGAIESAQTPETEQSMPNTYTVAAYYFPNYH
                     PDARNAKQHGEGWTEWELVKASRPRFEGHQQPNVPLWGYTDESDPEVMAAKIAAAADH
                     GLDVFIFDWYWYDDGPFLQRGLEDGFMKAANRDRMKFALMWANHDWMDIHPARSDKES
                     AVLYPGTVTPETFEQMTDYIIETYFKHPCHWQIDGRPYFSVYELHTLIQSFGSLDATR
                     AALDRFREKTIAAGFPGLHLNAVTFG"
BASE COUNT          696 a         1075 c          996 g          624 t
ORIGIN      
        1 ggttagcgcc tgccccgtcc ccgcatcttc aacaacgaac tcatcgagcc atatggcgcc
       61 ggtggtatcg ccgctattgc tccacagggt ggggaatacg gcaaaggcgc agaaacgcgc
      121 ctcgtcagga aagattgaga tcgtgcattc gaacgattgc cacgaaccat caccggtggt
      181 gatggtatcg aggttacggc cggacatcca tttgcccgcg gcatcgaaat gccggatcga
      241 ggtcaggaag cgctgacccg gctctttcgt tttgtaccag aaacgcacac gcaggccctt
      301 ctccggtatg ggcaagcgat cggcataatc gagcgagata ctcttgtccg tatacgggtc
      361 gttcagaaat cgcgacgcat ggccgctctt ctttgtctct ttatctacct cacctccccg
      421 ccacttcggc aagccggtcc aggtcagccc gataccatcc agaggcgcag ggtcatatgg
      481 cgaaatgtgg tgccggctga agttgacgag atatttgtcc atgacttcgc gccgttgatc
      541 agggtcgctg agcttctgat actcgaacac aagtccggga gagaagccga aggccgagac
      601 gcaggtcatt cgatccggga gttcgaaatc gtaaacctcg acctccaggg gcgtctccgc
      661 catgaatcca tccgcagtca cgcgaagcga gccgcgatag atgcccgcag gttgatcttt
      721 gggcgcattc acccgaatcc agagcggctg gttcatgccc gccgccacat cgatgggccc
      781 acggaacggc ggcagcggat caggccacgg cgcggctacg ccggtctcat cggtggggta
      841 cacgacatcg acataacgca cccgcagcac ttccacagcc gatgcaggca gcaccgcgcc
      901 agcgggaccg gccagatcgc ccgcagtaac ggtcaatccc gtcagcgggc gctggggcgt
      961 aatcacgaac tggacggcct cggcctcatt acgggcgagg cgcaattcga ccgcatcggc
     1021 ctgacggctg ggtgcagggc gggtctgggc gatcttccat ccggaggaag cgcgccacag
     1081 cgcgacgtcg gctgtagaac cgggcacacg ctctccatag gcggtctcga agaggtgggg
     1141 tacgtggaaa tcggcgtgga atgcaaacac aggccccgga tcgagcgcga cttcgagagc
     1201 gaaggcgccc gaacccggca cttcataggg gatctcgaga acactggttc cggcgggcag
     1261 ctccaccggc gggcaatcct gctccagcct tccaccttcc cccgtgagcg aaaccgtggg
     1321 cgtaacaaac acgggagtat cagcgcggtt gtcgaccagc aggcgggccg tgttcgcgcc
     1381 gccaggcagg acatcaccaa gggagtccac ggtcacgttg atgcccgggg tcgcggctgt
     1441 cactgcgaca aagcgggtcc tgccttgaag cgtgacgggc gtgccgtcca gtgttgcccg
     1501 gtatgtatag tcgttcagcg tgaaggcaca ggaacccgcg cggccctccg cagcgtcgcc
     1561 cggcaccccc cgcagccgga tccatatttc gctcgcgggg aacaggttgt ccggcaaggt
     1621 gaacgacgcg cccccatccg cggcagcctc gccgatgggc gtccaatcat tgccatcccg
     1681 ggaggcttcg gcggccaggc ggccggctga atagtaggaa aggctcacct cgacgctcgc
     1741 accggtctgc tggcggccag ccagctggtg ccggtagaca agctcgctgc cggcgccaaa
     1801 ggtccatcga ttcgtattga aatggcattg ctgccgaatc agcggccggc tttgattacg
     1861 cgaagcggaa taatgcggcg ctgagaagat gtaagagagg ccatcgatgc gttcaccctc
     1921 tcccagacgc atgccctcct gctcgacgta cagagggtgc acggcgaaaa tggccacatt
     1981 atcgaaggcc attgatccgt tatgctgcca ctgaccgaac cgaagccccc cctcccctgg
     2041 cgcgtctgga gtgaagaaga ccgagctgta tgacttccat tcccccgtga gcacgcccag
     2101 atcccggttg cagaagctcg ggccggtgat agccgtcccg ccgctgccgc cccagtttcg
     2161 tgcgtcaaat gtcaccatat acaccgacga cggttcgagt gcgatcgcgc cggatcgcca
     2221 gaagttgctg tcgttgccgg tacccgtcac ctggatggcg cgagaaccac tggacgcctc
     2281 gccatcgagc cattggcccg atccgccgga cagagtccag ccggccggac cgtcctgtcc
     2341 ttcctcaaaa gatgcgttgg gcacatccac ggtttgagcc aggctgaccg gcactgcaaa
     2401 cgctgcaacg gcccccagaa ttaacgtgat acgtagccat cgctcgatca ttaatccgtc
     2461 ctccccgatg gtggattctt ctgagatgtc atcgaaggca ttgtagcagt ctcgacatgg
     2521 gatttcttcc atgcacacac ttgcggtcag tacggtgctg cttgccctgc cttttgtgca
     2581 gaattttgcc gcagtcggcg gtttgcttac agttttcgca gcggctaagc tagggtccaa
     2641 ctttggcctg gagaaatacc atgtataccg cactgttgac attgagcttc ttatgcacgt
     2701 acggcgcgat tgaatcggcg caaacacccg aaacggagca gtccatgccc aatacgtaca
     2761 cggttgccgc ctattacttc cccaactacc atccggacgc tcgcaacgcc aaacaacacg
     2821 gcgagggctg gaccgaatgg gaactggtca aagcgtcccg gccgcgcttc gagggacatc
     2881 aacagccgaa tgtacccctc tggggctata ccgacgaatc cgatcccgaa gtgatggctg
     2941 caaagatcgc cgctgcggcc gaccacggac tcgacgtctt catctttgac tggtactggt
     3001 atgacgacgg tccgttcctg cagcgcggcc tggaagacgg cttcatgaaa gccgccaacc
     3061 gcgaccgcat gaaattcgcc ctgatgtggg ccaatcacga ctggatggac atccatcccg
     3121 cgcgctcgga caaagagagc gccgtgctct atccgggtac ggtgacgccg gagacgttcg
     3181 agcagatgac cgattacatt atcgagacct atttcaaaca tccctgccac tggcaaatcg
     3241 acggccgccc ttatttctcg gtctatgaac tgcataccct catccagagt ttcggaagcc
     3301 tcgacgccac ccgcgcggcc ctcgaccgct tccgggaaaa gaccatcgca gcggggttcc
     3361 cgggcctgca cctgaacgcc gtgacctttg g
//
LOCUS       DAOPBQ010000411         3383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22460_length_3383_cov_3.168870, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000411 DAOPBQ010000000
VERSION     DAOPBQ010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22460_length_3383_cov_3.168870"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PKW60_12045"
     CDS             <1..525
                     /locus_tag="PKW60_12045"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HPK00210.1"
                     /translation="GFLAEAMGVHTGFPFGQYVYSDALFPAVWKVPVVMACAWWTLSG
                     YVREMLRGLFARRIAEAFCAAIWMTALDFVIDPLAAGSLGYWKWAHPGVYYGIPLLNF
                     AGWLGVSLLIFLVIPPPRRENRPARSIGLSMIVFFLFPATAHAHWGVVVAGAGLCLAH
                     FLVCRICSPRTFFS"
     gene            598..978
                     /locus_tag="PKW60_12050"
     CDS             598..978
                     /locus_tag="PKW60_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPK00211.1"
                     /translation="MLFGKSAQRTPKGGDVFSKGLGNLGNLGGLMKQALEMKGRMEEM
                     KEQLASETLEASSGGGMVRVTLNGKFEILSLKIDPEIIDKNDAEALETLVRAAINEGV
                     RAVQDRVHAKMAELTGGLDIPGLG"
     gene            1063..1440
                     /locus_tag="PKW60_12055"
                     /pseudo
     CDS             1063..1440
                     /locus_tag="PKW60_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
     gene            complement(1492..2016)
                     /gene="cas5c"
                     /locus_tag="PKW60_12060"
                     /pseudo
     CDS             complement(1492..2016)
                     /gene="cas5c"
                     /locus_tag="PKW60_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
     gene            complement(2032..>3383)
                     /locus_tag="PKW60_12065"
     CDS             complement(2032..>3383)
                     /locus_tag="PKW60_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HPK00212.1"
                     /translation="IIYVIPYTSIIEQTADVLRHHLGVENVVEHHSNLDPETQAKAAR
                     LASENWDAPIIVTTSVQFFESLLGARSSRCRKLHNIANSVVILDEVQLLPPELLEPCT
                     DLLRQLVERYGVTIVLSTATQPSLPNIENVQEIIEASARLPERLRRVVYELPNVLENR
                     SNSWPEVAKELEAYAQVLCVVNTRRDCLELFRCLPPGTVHLSASMCGAHRSQVIESIK
                     AKLRSGAPIRVVSTQLVEAGVDIDFPVVYRALAGLDSIVQSAGRCNREGRQAAGGKVV
                     VFIPPRSAPKGILRKAEDAARVMLASPISLEGSEIYPRYFREFYSRVEGRFPYQTLLV
                     QNARSLEFQFREAAAQFHIIDQAHHSVFVRHGEGDALIETLRRQIRDDMPYGWLLRKL
                     QRFSITLPQGQLNTLLHNGQIEELTTGLFVWNGRYDEITGADIFNEDGILSAKDTVL"
BASE COUNT          706 a          991 c          949 g          737 t
ORIGIN      
        1 gggtttctcg ccgaagcaat gggcgtgcac accgggtttc ccttcggaca atacgtttac
       61 tccgacgcgc tgttcccggc ggtctggaaa gtgcccgtcg tcatggcatg cgcatggtgg
      121 acgctatccg gctacgtccg cgagatgctc cgaggtctgt tcgcgcggcg tatcgccgaa
      181 gccttctgcg ccgcaatttg gatgaccgcg ctggatttcg tcattgaccc cctcgccgca
      241 gggtcattgg gctactggaa atgggcccat cccggcgttt actacggcat tcccctcctc
      301 aattttgccg gatggctcgg cgtctccctg ctcatctttc tggttatccc ccctccccgc
      361 cgcgagaacc gccctgcgcg aagcatcggc ctcagcatga tcgtcttctt tctcttcccc
      421 gcaacagcgc acgcccactg gggcgtcgtc gtggccggcg caggactgtg cctcgcccat
      481 ttcctcgttt gccgaatctg ctcccccaga actttttttt cgtaaatccg cccggccctc
      541 ctgcctgctt gttgcccctc gatgccgcga tggctatact tttcggcggg catagtcatg
      601 cttttcggca agtcggctca acgcacacct aaaggaggcg atgtgttttc aaaaggattg
      661 gggaatctgg gcaacctggg aggcctgatg aagcaggccc tggagatgaa gggccggatg
      721 gaggaaatga aggaacagtt ggcctctgaa accctcgagg cgagttccgg cggaggaatg
      781 gtccgcgtca cgttgaacgg caagttcgag attctgtcgc tgaagatcga tccggaaatc
      841 atcgacaaaa acgacgcaga agccctggaa acgctcgtcc gcgcggccat caatgaaggc
      901 gtgcgcgccg tgcaggaccg cgtccatgcg aaaatggccg agcttacggg cggtctcgac
      961 atcccggggc ttggctgagt gcgcacggtc ttctttggaa cgcctgagct tgcgacgccc
     1021 tcgttggcgg cgatcgcgcg cggtcatgag gtgacggcgg tcgtgtgtca gccggacaag
     1081 ccgcagggac gcagcagcaa gcccgtgccg ccgccggtca aggcatgggc gcaggatcgc
     1141 ggcattgccg tcatgcagcc gacaaggctg aacgatggcg cgttcgaggc gtggctgcgc
     1201 gaacagcagc cggatatttg cgccattgcc gcgtacggac gcttgctcaa acaaccgatt
     1261 cttgacgttc cgccgcatgg gttcatcaac atgcacccga gtcttctgcc gcgtcatcgc
     1321 ggtccttcgc cgatcacgtc ggccctgctg gagggcgatg cagtcaccgg cgtgtcgatc
     1381 atgcggctga cgcttgaagt cgattcgggc gatctgctgt tgcaggaggc gacgccgatc
     1441 ggtcccgagg aaaacaacat cgaactcacc gagcgattgt cgcacatggg cggcgaactc
     1501 acgacaaccc agatagggct ggtgaaaatg ctgccccttg cgcgcccgcc gctcaaacat
     1561 cgcggcatat ttggcttctg tttcatccgc gcgggtatcg gccgagacga gtgcggtttt
     1621 ctcttctgaa tccaccagcc actccggtac cggattgtgc acctgctgac gtttggcggg
     1681 ggggatgaaa tcaaataccc cataaagacg gtatttcaca tccacaagca gcatggctgc
     1741 tcgctgctgg cgatcatcct caataaatat ctctctgccg cgaggcgagg caactgcaga
     1801 aacctcattg cgtcgaatat tggcccagcg aattggcgcg agcacctcga ttttttcaat
     1861 tcgccaccga atggccggct tccacaaaat ggcatcgaaa atggcgcggg ccgcggacgg
     1921 agtaatgaca tcgtagctca cgcgttcaac tttcatttcc ggccgtgtga aacaggcgta
     1981 ctcacccgat acttccaaac agaatcctct actcatacct cttccctttc ctcataatac
     2041 ggtgtctttt gcggataaaa tgccgtcttc attaaaaata tcggcgccgg ttatttcatc
     2101 gtaacggccg ttccaaacga aaagtccggt cgtcagttct tcgatttggc cgttatgcaa
     2161 taacgtgttc aattgccctt ggggcaaggt aatggagaag cgttgcaatt tacgcaggag
     2221 ccagccgtat ggcatatcat cgcgaatttg ccgacgcagg gtctcaatca atgcatcccc
     2281 ctcaccatgg cgaacaaata ccgaatggtg cgcctgatca atgatatgga attgcgcggc
     2341 cgcttctctg aactgaaatt ccagagatcg agcgttttgg accagcaggg tttggtaggg
     2401 aaagcgcccc tctacacggg aataaaactc gcggaaataa cgtggataaa tctcggaacc
     2461 ttccaaggag atggggctcg caagcatgac acgcgcggca tcttctgctt tgcgtaatat
     2521 tcctttgggc gccgatcggg gcgggataaa gaccaccact ttgccgcctg ccgcctgccg
     2581 gccttcgcga ttgcacctgc cggcggattg gacaatcgaa tctagaccag ccagcgcccg
     2641 atatacgacc ggaaagtcaa tatctacccc cgcttccact aactgggtgc tgacaactcg
     2701 aattggtgct cccgaccgca atttcgcttt gatggattca atcacttgcg accggtgcgc
     2761 cccgcacatg ctggctgata agtgaaccgt gcccggcggc aggcaacgaa aaagttcgag
     2821 gcaatcgcgc cgggtattca ccacgcaaag cacttgcgca tacgcctcaa gttccttggc
     2881 cacttccggc cacgaattgg agcggttttc cagaacattc ggcaactcgt acaccacccg
     2941 acgaaggcgt tccggcagac gagcggatgc ctctatgatt tcttgcacgt tttcaatatt
     3001 cggcaggctg ggttgagtgg ctgtactgag tacgatggtg acaccatatc gttcgacaag
     3061 ctggcgcagc agatccgtgc aaggttccaa taattcgggt ggcaacagtt gcacttcatc
     3121 cagaatcacc acgctgttgg cgatgttgtg caactttcga cagcggctgg accgggcgcc
     3181 aagcaaagac tcaaagaact ggacactggt ggtgacgatg atcggggcat cccaattttc
     3241 ggaagccaaa cgggccgcct tggcttgtgt ctccggatca agattggaat ggtgttccac
     3301 cacgttctcc acccccaagt ggtgtcgtaa cacatcggcc gtctgctcaa tgatggaggt
     3361 gtagggaatt acatagatga tcc
//
LOCUS       DAOPBQ010000412         3363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22611_length_3363_cov_4.967050, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000412 DAOPBQ010000000
VERSION     DAOPBQ010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22611_length_3363_cov_4.967050"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKW60_12070"
     CDS             complement(<1..419)
                     /locus_tag="PKW60_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPK00213.1"
                     /translation="MSRVPCPLHDAALRHPDAAAFVDASGQAITFADCDAKASAVAAA
                     LRSAGLSAGARIALVEPLDTWSPAILFGAFRAGCAAYIPNARLPRPALLEHLAHTNCR
                     CVVASAQAGELPHRVAPAGLLDHPDRLTSAEVELDAPA"
     gene            complement(416..1513)
                     /gene="menC"
                     /locus_tag="PKW60_12075"
     CDS             complement(416..1513)
                     /gene="menC"
                     /locus_tag="PKW60_12075"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HPK00214.1"
                     /translation="MRIARADVFRYDLPLVRPLALRDCVCTSRSGLLLRFENEDGAFA
                     FGEAAPLPCFSRESLDETRQQALMLAKSLRGTTLPPRLELVTDHFERWLMPFELVNSL
                     KFAVQSALLQLLTAEHAIPAFGLASQPHRTSIPINGLLWGTRDAVLARAEALLREGYS
                     TLKLKVGRESPAEDAETVRALCALCTGQCAVRLDAGRLWELPDAMAFARAIEGCTIEY
                     IEEPLRDPADLVAFAGRSGLPVALDETVAEQGECELHQWRGARAAVIKPTLLGGFEIA
                     MFLARKAINLGMMPVISSSFESPVGLIALGQLAAFVNEGDVAAGLDTWNWFAANLLDA
                     PLPIEQGRLNLTRAADLLRRLNTGKLEKLEP"
     gene            complement(1510..2403)
                     /locus_tag="PKW60_12080"
     CDS             complement(1510..2403)
                     /locus_tag="PKW60_12080"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013035465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HPK00215.1"
                     /translation="MMDELTPTDAWIEAARPRTLGAAVSPVIVGTALALGDRASHLPA
                     AFCALMGALLIQIATNFANDYFDYLKGADTAERKGPLRATQSGLVKPIHVLRAALVTY
                     LLAFLPGAYIVWRGGWPFIAIGVLSVVFGIIYTAGPFPLAYTGLADVFVLVFFGPVAV
                     AGTYYVQTLTLQWAPFVAGLGPGLLAVALLTVNNLRDVNEDRKANERTLAVRFGRGFA
                     RAEYLFCVVAACVGVPAYFYVHEGDLWFMLCPAVLIFLAAPAMRIVFLRRDGPALNRA
                     LARTGGMLLLYSVLFSVGSIL"
     gene            complement(2446..3267)
                     /gene="menB"
                     /locus_tag="PKW60_12085"
     CDS             complement(2446..3267)
                     /gene="menB"
                     /locus_tag="PKW60_12085"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HPK00216.1"
                     /translation="MASIIWSRAGAFTDIRYEKAEGIAKITINRPEVRNAFRPLTVDE
                     MSQALSDARFDPDIGVIILTGEGEKAFCSGGDQRIRGDAGYVDDTGRHRLNVLDFQRQ
                     MRTCPKPIIAMVAGYAIGGGHVLHLMADLTIAADNAVFGQTGPKVGSFDGGYGASYLA
                     RIVGQKKAREIWFLCRQYSASQALDMGLVNAVVPYERLEEETVQWCREILANSPTALR
                     CLKAALNADCDGQAGLQELAGNATLLFYQSPEAQEGRNAFNEKRPPDFGKYPRHP"
BASE COUNT          628 a         1100 c         1065 g          570 t
ORIGIN      
        1 gccggcgcgt ccagttctac ttccgcggaa gtcaggcggt ccggatggtc cagcaggccg
       61 gcaggcgcta cacgatgcgg cagttctccc gcttgcgcgg atgccactac gcaacggcag
      121 ttcgtatggg cgagatgttc gaggagcgcc ggcctgggaa ggcgcgcgtt cgggatgtag
      181 gcggcgcatc cggcgcgaaa cgcgccgaag aggatcgccg gagaccaggt atccagcggt
      241 tccacgagcg cgatccgagc ccccgcgctc aggccggccg agcggagcgc tgccgcaacc
      301 gccgaagcct tcgcatcgca gtccgcgaac gtaatcgcct gtccggacgc atcgacgaac
      361 gcggctgcgt ccgggtggcg cagcgcggcg tcatgaaggg gacagggcac gcggctcatg
      421 gctcgagctt ttccagcttg ccggtattga gtcgccggag cagatcggcg gcgcgcgtga
      481 ggttcagccg tccctgttcg atgggcaggg gcgcgtcgag caggtttgcc gcgaaccagt
      541 tccaggtgtc gaggcctgcc gccacatcgc cttcattcac gaacgcagcc agctggccca
      601 gggcaatgag gcccacggga gactcgaatg aggagctgat cacgggcatc atgcccaggt
      661 tgatcgcttt tcgcgcgaga aacatcgcaa tctcaaaccc gcccagcagc gtgggtttta
      721 tcacggcggc ccgggcgccg cgccactggt gcagctcgca ttctccctgc tcggcgacgg
      781 tctcgtcgag agccaccggc aatccggagc ggccggcgaa tgccacgagg tctgcggggt
      841 cccggagcgg ttcctcgatg tattcgatgg tgcatccctc aatggctcgg gcaaacgcca
      901 tggcgtcggg cagttcccag agccggccgg catcgagccg cacggcgcat tggccagtgc
      961 acagggcgca gagtgcgcgc acagtctctg cgtcttccgc gggcgattcc cgccctacct
     1021 tgagcttgag ggtggagtat ccctcgcgca gcagcgcctc ggcccgcgca agcacggcgt
     1081 cacgggttcc ccagagaagc ccgttgatcg ggattgacgt ccggtgcggc tgcgaagcca
     1141 gtccaaacgc gggaatggcg tgttcggccg tcaaaagctg caacagggcg ctctggaccg
     1201 caaatttcag cgaattcacc aattcgaagg gcatcagcca gcgttcgaaa tgatccgtca
     1261 ccagttcgag gcgcggtggc agcgtggtac cgcggagcga tttcgccagc atcaaggcct
     1321 gctggcgggt ctcgtccagg ctttcgcggc tgaaacacgg caacggcgcc gcttcgccga
     1381 acgcgaacgc gccgtcttcg ttttcaaacc gcagcagcag accggaccgt gaagtgcaga
     1441 cgcaatcccg caaggccagc gggcggacaa gcgggaggtc gtatcgaaag acgtctgcgc
     1501 gggcaatcct catagaatgc tgcccacaga aaagagaacg ctgtacagca ggagcatgcc
     1561 gccggtgcgg gccagagcgc ggttcaaggc cgggccatcg cgacgcagaa acacgatacg
     1621 cattgccggg gcggcgagaa agatcaagac cgcgggacac agcatgaacc acaggtcgcc
     1681 ctcgtgcaca taaaaatacg ccggcacgcc cacgcaggcg gctaccacgc aaaacaagta
     1741 ttcggcacgt gcgaatccgc gtccgaaacg caccgccagg gttctctcgt ttgccttgcg
     1801 gtcctcgttc acgtcgcgca ggttgttcac agtaagcagg gccaccgcga gcaggccagg
     1861 gcccaggccc gccacaaacg gcgcccactg taaggtcagc gtctgcacgt aatacgtccc
     1921 ggccaccgct accggcccga aaaacaccag cacaaacacg tccgcgaggc ccgtataggc
     1981 caatggaaac ggccccgccg tgtagatgat gccgaagacg accgaaagca cgccgatcgc
     2041 gatgaatggc cagccgccgc gccagactat gtatgccccc ggaagaaagg ccagcagata
     2101 cgtcaccagc gcggcacgca gcacgtggat gggcttcacc agtcccgact gtgtcgcgcg
     2161 cagcgggcct ttccgttccg cggtatccgc ccctttcagg taatcgaagt agtcgttggc
     2221 gaaattcgtg gcgatctgga tcaacaacgc tcccatgagc gcgcagaacg cggcaggcag
     2281 gtggctggcg cggtctccca gcgcgagcgc cgtgccgacg atgaccggcg acacggccgc
     2341 ccccagcgtc cttgggcgcg ccgcctcgat ccaggcgtct gttggtgtga gttcgtccat
     2401 catggtgctt ggaaccgccg tcgccgcggg ccgagcgtac ctccgttacg gatggcgtgg
     2461 atacttgccg aaatccggcg gccgtttctc attaaatgcg tttcgccctt cctgggcctc
     2521 gggggactgg taaaacagca gcgtcgcatt tccagccagt tcctgcaatc cagcctgtcc
     2581 gtcgcaatct gcgttcaggg ccgctttgag gcagcgcagg gccgtgggcg aatttgccag
     2641 gatttcgcgg caccattgca ccgtttcctc ctcgagccgc tcgtatggca cgaccgcgtt
     2701 gaccagcccc atgtccagcg cctggctggc ggaatactgc cggcacagaa accagatctc
     2761 ccgggccttt ttttgtccca cgatgcgggc caggtaactt gcgccgtatc ccccgtcaaa
     2821 tgagcccact ttcgggccgg tctgcccgaa aaccgcattg tccgcagcga tggtcaggtc
     2881 cgccatcaaa tggagcacat gccccccgcc gatggcatat cccgcgacca tcgcgataat
     2941 gggtttgggg caggttcgca tctggcgctg aaaatccagc acattgaggc gatgccgtcc
     3001 cgtgtcatcg acatacccgg cgtcgccccg gatcctctgg tcgccgcccg agcagaaggc
     3061 tttctcgcct tcccccgtta agatgatgac tccgatgtcc ggatcaaagc gcgcgtccga
     3121 aagcgcctgc gacatctcat cgacggtgag cggccggaac gcgttgcgca cttccgggcg
     3181 gttgatggtg atttttgcaa tgccttccgc cttttcgtag cggatgtccg taaaggcgcc
     3241 ggcgcggctc cagatgatgc tggccatcgc tgactctccc gtatctgtgg atgctgtggc
     3301 tagtgcgcgg gcgttccgcg cagcccgtca agccatttcc gcaaacgcgt agtatagtcc
     3361 ccc
//
LOCUS       DAOPBQ010000413         3360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22634_length_3360_cov_3.593949, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000413 DAOPBQ010000000
VERSION     DAOPBQ010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22634_length_3360_cov_3.593949"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..1576
                     /locus_tag="PKW60_12090"
     CDS             326..1576
                     /locus_tag="PKW60_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00217.1"
                     /translation="MSQGTTRRQFFRNAAVGAAAISMSAASYARIKGANERISIGMIG
                     CGDRGRNAHMSNIHRYSEEQNVEITAVCDPWKVNRERAAADTKSWYGREARQFVSYRD
                     LIALEDIDAVMIASCDHLHTTHMKAAAEAKKDIYCEKPLAMDFEQLKAACDAVRANNL
                     VFQAGTQLRSLPSMTGAREVYQSGRLGTVGRIEQCRNAARPYWYSYVKDVDEADVDWN
                     EFLLDREPRPFSAALYSGWYGYRDFSDGPVPGLGSHFIDLVHYITGATFPTSAVCMGG
                     IYTWKDDYNFTCHDHVHALWEYPEGFMVSYSTNFGNGSGNSFKFFGDAAVLDCQDWNQ
                     PVLTSDGINTAKGETFEKENVAPVERADHFLDWLQCLRSRETPNASLDAGYQHAVAVL
                     LAMRACNTGQRQIYDAEKREMRSA"
     gene            1614..2522
                     /locus_tag="PKW60_12095"
     CDS             1614..2522
                     /locus_tag="PKW60_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPK00218.1"
                     /translation="MMKSTRREFLGRSAQLGAAGLAAVTLGASAAESAPASPWKIGIY
                     TRPWDKLDYRVAFDAIAAAGYKYAGLMTTNNEEHLVISAKTTPEEAGKVAEELTQRGL
                     LVPSVYGGGIPVNESLEAGIAGMRRLIDNCATVKAENLLMGGIGDENLYALYYKAIAE
                     TCDYAAEKGIGISVKPHGGLNATGPQCRKTVEETVGHKNFRIWYDPGNIYYYSNGELS
                     PVDDAATVDGLVVGMCVKDYVHPKQVDVTPGDGMVDFAKVLARLKQGGFTKGALVVEC
                     LKPGDEEQLLAEAKRAREFVEQLVAQ"
     gene            2577..>3360
                     /locus_tag="PKW60_12100"
     CDS             2577..>3360
                     /locus_tag="PKW60_12100"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HPK00219.1"
                     /translation="MRSSMLCVSLGIATALAGGAAATVDYAADVQRNITWAESAFAVH
                     RQDATPGNRLVVVHEDGAGDTKLRRCSFGGPLRLGERVYEHGIGVNSHSVLRVSLAEP
                     AREFAADIGLDRNVDGSPASVRFQVLVAGETVFSTEVMRPGDPVQSIVVPLNNAQEFD
                     LVLDIGGDNRSFDQGDWGDARVTLASGAVLWLDELDYTPPWLGQPPFSFVYGGKPSSE
                     LLGAWEQSLDDSPPGVEPRTRTLTLRDPATGLEVRAICTLYPD"
BASE COUNT          689 a          973 c         1107 g          591 t
ORIGIN      
        1 gatcagccac tccggcggcc ttcaggatgc catccagcgg cgggccgtcc gcgagcacgg
       61 ttttggacac ggtcagtgcg atgaaaacca tgaaaagaac cgccagtcgt ggcatagcgc
      121 gtactccttg agccggtgca ggtgagagtt gatttttctg ccgcgaaaac catagcgaaa
      181 ccgcgcgttg cgtgcaagcg gcacaaagct gagagtccag agaaatgccc agagcggcgc
      241 gttgacgttg ctcttttatt gagaggcgtc agggggtatg attagaagag tcttgtcagt
      301 cacgcaagcc agaggaggta cgagcatgtc acaaggcacg acccgtcgtc agttttttcg
      361 taacgctgct gttggcgcgg cggcgatcag catgagcgcg gcgagctacg cgcggatcaa
      421 aggcgcgaat gaacgcattt ccatcggcat gatcggatgc ggcgatcggg gccgcaacgc
      481 ccacatgtcc aacatccacc gctattcgga agagcagaac gttgaaatta ccgcggtctg
      541 cgatccctgg aaggtgaacc gggaacgagc cgccgcggac acaaagagct ggtacggccg
      601 tgaagcccgc cagtttgtat cgtaccgcga tctcattgcg ttggaagaca tcgatgcggt
      661 gatgatagcc tcgtgcgacc atctgcacac cacccacatg aaggccgccg ccgaggccaa
      721 gaaggacatc tattgcgaga agccgctcgc catggatttt gagcagctga aagcagcctg
      781 tgatgcggtc agggcgaata acctggtgtt ccaggccggc acgcagctcc gcagcttgcc
      841 cagcatgacg ggtgcgcggg aagtctatca gtcgggaagg ctgggaaccg tcgggcgcat
      901 cgagcagtgc cgcaatgcgg cgcggccgta ctggtattcc tacgtgaagg atgtggacga
      961 ggcggacgtg gattggaacg agtttctgct ggaccgcgaa ccccgtccgt tcagtgccgc
     1021 cctgtattcc ggttggtacg gctaccggga cttctcggac ggccccgtac cgggattggg
     1081 aagccatttc atcgatctgg tccattacat caccggggcg accttcccga cctccgcggt
     1141 ctgcatgggc gggatctaca catggaaaga cgactacaat ttcacgtgcc atgaccatgt
     1201 gcatgccctg tgggaatacc ccgagggatt catggtgtcg tactcgacca atttcggcaa
     1261 cggtagcgga aactcgttca agttctttgg cgatgccgcc gtgctggact gccaggactg
     1321 gaatcaaccc gtgctgacgt cggatgggat caacacggcc aagggcgaga cctttgaaaa
     1381 agagaacgtg gcgcctgtag agcgcgccga ccacttcctg gattggctcc agtgcctgcg
     1441 cagccgcgag accccgaacg cctcgctcga cgccggttac cagcatgccg tcgcggtact
     1501 gttggccatg cgggcctgca acaccggcca gcggcagatc tacgacgctg aaaaacgcga
     1561 aatgcgcagc gcctgatcct taaaagcgca gccagtacga tgaagggaat cgcatgatga
     1621 agagtactcg acgagaattt ctcggccgct ccgcccagct cggagcggcc ggtcttgcgg
     1681 ccgtgacact gggggcctcg gcagcggaat ctgcgccggc ctccccgtgg aagatcggca
     1741 tctatacccg cccgtgggat aaactggact accgcgttgc gttcgatgcg atcgccgcgg
     1801 cagggtacaa gtatgcgggg ctgatgacca ccaataacga agagcacctg gtgatctcgg
     1861 ccaaaaccac gccggaagag gcgggcaagg tcgctgaaga actgacgcag cgcgggctcc
     1921 tggttccgtc ggtatatggc ggcggcatcc ccgttaatga atccctcgaa gcagggatcg
     1981 ccggcatgcg cagactaatc gacaactgcg caaccgtaaa agccgagaat ctgctgatgg
     2041 gcgggatcgg ggacgaaaat ctgtatgcgc tttactacaa ggccatcgct gaaacctgcg
     2101 attacgccgc ggagaaaggc atcgggatca gcgtcaaacc ccacggcgga ctcaatgcaa
     2161 cggggcccca gtgcaggaag accgtcgaag aaacggtggg ccacaagaat ttccggatct
     2221 ggtacgaccc gggcaacatc tactattact cgaatggcga actcagcccg gtggacgacg
     2281 ccgcgacggt ggacggcctg gtcgtgggta tgtgcgtgaa ggattacgtg catcccaagc
     2341 aagtcgacgt cacgccgggc gacggcatgg tggatttcgc caaagtgctg gctcgcctca
     2401 aacagggcgg attcacaaaa ggcgcgctgg tcgtggagtg cctcaagccc ggcgacgaag
     2461 agcagttgct ggccgaggca aaacgcgccc gcgaattcgt cgagcagctc gtcgcgcaat
     2521 aagggcggcg ctggcagcgt gccccgaagc gcgtgtgcgc gcggagaagc tgggccatgc
     2581 gtagtagtat gctttgcgtg agtctgggga tcgcgacggc tcttgccggc ggcgcggcag
     2641 ctacggtgga ctacgccgcc gatgtgcagc gcaacataac gtgggcggaa agcgcgttcg
     2701 ctgttcacag acaggacgcg acccccggga acaggctggt ggtggttcat gaggacggcg
     2761 ccggagatac caagctccgg cgctgcagtt ttgggggccc gctgcgcctg ggcgagcggg
     2821 tctatgaaca cggcatcggc gtgaattcgc acagcgttct acgagtgagt ctggcggagc
     2881 cggcccggga attcgccgcg gacatcggtc tcgatcgcaa tgtcgacggc agccccgcgt
     2941 cggtgcggtt ccaggtgctc gtggccgggg agacggtgtt ctcgacggag gtgatgcgcc
     3001 cgggcgatcc ggtgcagtcc atcgtggtcc ccttgaacaa cgcccaggag ttcgacctgg
     3061 tgctggacat cggcggcgac aatcggagct ttgaccaggg ggactggggc gatgcgcgag
     3121 taacactggc cagcggcgcc gtcctgtggc tggacgaact ggattacacg ccgccctggc
     3181 ttggccagcc tccgttttcg tttgtgtatg gcgggaagcc ctcttcggaa ttgctcggcg
     3241 cgtgggagca gtcgctggat gactcgccac ccggagtcga gccgcgcacg cgcaccctga
     3301 ccctgcgcga tccggccacg ggcctcgagg ttcgcgccat ctgcacgttg tatcccgacg
//
LOCUS       DAOPBQ010000414         3354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22688_length_3354_cov_4.118824, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000414 DAOPBQ010000000
VERSION     DAOPBQ010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22688_length_3354_cov_4.118824"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKW60_12105"
     CDS             <1..468
                     /locus_tag="PKW60_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013961697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HPK00220.1"
                     /translation="ELLEPLRELFKDEVRVLGRELGLPGEIVDRHPFPGPGLGVRCVG
                     AVSRERLDTLREADAIFVDEIKQAGLYHDIWQALVCLLPVRSVGVRGDERSYEEACSL
                     RAVISEDAMTADWFRFPADVLERTASRICSEVPRINRVLYDITSKPPGTIEWE"
     gene            563..1360
                     /locus_tag="PKW60_12110"
     CDS             563..1360
                     /locus_tag="PKW60_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPK00221.1"
                     /translation="MKVGILTASLPMDTKTALRKARELGAHGVQLWIADNDLDPRNLT
                     RSGREELIAYMTSLSLERAALCGDVGGFTDPPTVDERITRTKQMFELCVDLRTPILTT
                     HIGVVPDDPAGRGYSDLLGAVREVAEYAANRGCCLAIETGPESPESLAEFLSRVDSGG
                     ARVNYDPANLVMNGFNPLDGLYTLRDYIVHTHAKDGVARAGVDDAEVREVPVGEGDVD
                     FPRYLHILRDELGYSGYLTIERECGSNPVADLAAAVAYLKRQPGVAS"
     gene            complement(1412..2221)
                     /locus_tag="PKW60_12115"
     CDS             complement(1412..2221)
                     /locus_tag="PKW60_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPK00222.1"
                     /translation="MQAAAELHPVWQAFLATMFTWFMTMLGAIPVFFAKEVNPRVMDG
                     LLGMAAGVMIAASFWSLLDPAITLSEDMGYPGWGPPLVGFLAGGAALWGIDRILPHLH
                     PGLAMSDAEGVPTSWRRSTLLVLAITMHNIPEGLAVGVAFGAAAAGIPGATAAGAVAL
                     ALGIGLQNLPEGTAVAMPLRREGLSRFKSFMYGQLSGAVEPIAGILGAAVVIIMRPFL
                     PYALAFAAGAMIYVVAEELIPEAQRRGDTDIPTIGVMVGFAIMMFLDVSLG"
     gene            2542..>3354
                     /locus_tag="PKW60_12120"
     CDS             2542..>3354
                     /locus_tag="PKW60_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00223.1"
                     /translation="MKANGWFVRVAAGLVMLVTMAVQAQDTFEQASASAAIAGHALSK
                     VHRWLHDVALPKIDPGTGLYKADEKWNYRDTAADCYPFLLWAAFVVDRDVMNGPVRSI
                     LAREQALCNTYGRIPVPWDFETGAPAKDTAWDEVVFQASEYVKDGLIAIVEVTGPDAW
                     FDRMRGIEDDLWARAHIETPFGNIPSEDIEVNGEQLQALARLYTMTGEEKYLDWGDRL
                     ADYYFAQDDFVPTRLRDHGCEIIGGLGLLHAVEVTHRPAKAAEHASRLKQIYD"
BASE COUNT          663 a         1057 c         1038 g          596 t
ORIGIN      
        1 gaactcctcg agcccctccg ggagctcttc aaggacgaag tacgcgttct ggggcgtgaa
       61 ctcggcctgc ccggcgaaat cgtcgaccgg caccccttcc ccggacccgg tctcggcgtg
      121 cggtgcgtcg gcgcagtgag tcgggaacgc ctggacaccc tgcgtgaggc ggacgccatc
      181 tttgtcgacg agatcaaaca ggccgggctg taccacgaca tttggcaggc tctggtgtgc
      241 ctgctcccgg tccggagcgt cggcgtgcgg ggagacgaac gatcgtacga agaggcgtgt
      301 tcgctgcgcg ccgtgatctc ggaagatgcg atgaccgcgg actggttccg attcccagca
      361 gacgtgctgg agcgcacggc cagccgcatc tgcagcgaag tgccgcgcat caaccgcgtg
      421 ctctacgaca tcacctcaaa gccacccggc accatcgagt gggagtgatc cgccaggcgc
      481 tttttctgac ccagttggaa gtcataaaaa aggtgccggg cagcgcggca gcgcggaatt
      541 tttcaccagg ggagggcatc tcatgaaagt cggaatcctc accgcatccc tgcccatgga
      601 taccaaaacg gccctccgca aagcgagaga actcggcgcg cacggcgtcc agctctggat
      661 cgccgataat gacctggatc cccggaacct cacccggagc gggcgcgagg aactcatcgc
      721 gtatatgaca tccctcagcc tcgaacgcgc tgctttgtgc ggtgatgtcg gcggatttac
      781 cgatcccccc accgtggatg aacgcatcac gcgcaccaaa cagatgttcg aactctgtgt
      841 cgacctccgg acacccatcc tcacgactca catcggcgtt gtcccggacg atccggccgg
      901 ccgtggctac agcgatttgc tcggcgccgt tcgggaagtg gctgaatacg ccgcgaatcg
      961 cggttgttgc ctcgccatcg agaccgggcc tgaatccccc gaatctctcg cggaattcct
     1021 ctcgcgcgtg gacagcggcg gcgcccgggt caactatgat cccgccaatc tggtcatgaa
     1081 cggattcaac ccgttggacg gcctgtatac tctgcgcgat tacattgtgc acacgcacgc
     1141 gaaagacggc gtcgcccggg ccggagtcga cgatgcggaa gtccgggagg tgcctgtcgg
     1201 tgaaggcgac gtcgattttc cccgatacct ccatattctt cgcgatgagc ttgggtattc
     1261 agggtatctg accatcgagc gcgaatgcgg gagcaatcct gtcgctgatc tcgcggcggc
     1321 cgtcgcttat ctgaaacgcc agcctggcgt cgcttcgtag tgccgggcaa tatagggccc
     1381 ggctcaggga gcatggagcg cggctccgaa atcaccccag cgacacgtcg agaaacatca
     1441 tgatcgcgaa tcccaccatg acaccgatgg tggggatgtc cgtgtcgccc cgccgctgtg
     1501 cttcggggat caattcttcc gcgaccacgt agatcatcgc gccggcggca aatgcgagcg
     1561 catacggcag aaacgggcgc atgatgatca caactgccgc gcccaggatc cccgcaatcg
     1621 gttcaacggc tcctgacaat tgcccgtaca tgaagctttt gaatcgggac aggccttcgc
     1681 gccgcagcgg catcgccacc gctgtgcctt ccggcaggtt ctgcagtcca atacccagcg
     1741 ccagtgccac cgctcccgcg gccgtcgctc cgggaatccc cgccgccgct gcgccgaacg
     1801 ccacgcctac cgcgaggcct tccggaatgt tgtgcatcgt gatggccagc acgagcagtg
     1861 tactccgccg ccaggaggtt gggacgcctt ccgcatccga catggcgagc cccgggtgca
     1921 ggtgcggcaa gatccggtca atgccccaca gggcggcacc gcccgccagg aaccccacca
     1981 gcggcggtcc ccagcccgga tagcccatgt cttcgctcaa ggtgatcgcc ggatccagca
     2041 gagaccagaa actcgccgca atcatgaccc cggccgccat cccgagcaga ccgtccatca
     2101 ccctggggtt gacttccttg gcgaagaaga ccggtattgc gccgagcatg gtcatgaacc
     2161 atgtgaacat ggtggcgaga aacgcctgcc ataccggatg aagttccgcc gctgcttgca
     2221 tacgccctcc gtacctgcgc gtgtccgcgc acctgctgtg tatctccgaa accagagtat
     2281 tgaccgctga attccgaaaa cgcaaacaaa ggacccgccg cagcggcgat agcaggggcc
     2341 ggtggcgcgt cctgatacca ttttatgcgg ttcagaagtg atgagccttc gtgggcgggc
     2401 cctctcagcg tgacctcgtg atttccggga tctcctgccc ggcgcgaaga tgacaagcat
     2461 gccgcgaagg atttacagtg tgttctccag gtttcgagga gagcggggtt ggctcaggtt
     2521 cagcagggag catagaaaat catgaaagca aacggatggt tcgtgcgggt ggcagcgggc
     2581 ctggtcatgc tcgtcacgat ggcggtgcag gcgcaggaca ccttcgagca ggcatccgcc
     2641 tcggctgcga tcgcggggca cgccttaagc aaagtgcatc gatggctgca tgacgtggcc
     2701 ctgccaaaga tcgacccggg gaccggtctg tataaagcag acgaaaaatg gaattaccgg
     2761 gatacggccg cagactgtta cccgtttctg ttgtgggctg cgtttgtggt ggaccgggat
     2821 gtgatgaacg gcccggtacg cagcatcctc gcccgggagc aggccctgtg caacacctac
     2881 ggccgcatcc cggtcccctg ggattttgaa acgggcgcgc ctgcaaaaga caccgcgtgg
     2941 gatgaggtgg tgtttcaggc ttcggaatac gtgaaagacg gactgatcgc catcgtggag
     3001 gtgacgggcc ccgacgcctg gtttgatcgc atgcgcggca tcgaagacga tctctgggcc
     3061 agggcgcata tcgagactcc gttcggaaac atcccctccg aagatatcga ggtgaacgga
     3121 gaacagctgc aggcgctggc ccgcctctac acgatgacgg gcgaagaaaa atacctcgat
     3181 tggggcgatc ggctcgccga ctactatttc gcgcaggatg attttgtccc gacgcgtctg
     3241 cgggaccacg gctgcgaaat catcggcggg ctgggcctgc tgcatgcggt cgaagtgacg
     3301 caccgtcccg cgaaagccgc ggaacacgcc tcgcggctga aacagatcta cgac
//
LOCUS       DAOPBQ010000415         3352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22702_length_3352_cov_3.524719, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000415 DAOPBQ010000000
VERSION     DAOPBQ010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22702_length_3352_cov_3.524719"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PKW60_12125"
     CDS             <1..300
                     /locus_tag="PKW60_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00224.1"
                     /translation="ELRDEVSEGERWQCYAEVRCDAKASSGVALAVGLYDATGKKHIV
                     QRRVPIADVAGADYKTIDLGTHALAPGMYFWAAPVNNPDEVDAIYVDRFYLLRKR"
     gene            426..1889
                     /gene="rfaE2"
                     /locus_tag="PKW60_12130"
     CDS             426..1889
                     /gene="rfaE2"
                     /locus_tag="PKW60_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPK00225.1"
                     /translation="MSYAELVEQFKKARVMVVGDIYLDENVYGAVTGVSLEAPIPVYE
                     VHEKRHNPGAAGNAACNVASLGATTYMVGYVGDDVNAAIVRREFAVRNVNTDYVVTDP
                     TRPTNTYGKLRAGGFNIPSQEILRTDTPSPTFISGAVEDQVVAHIEKLAPEVDAIVVV
                     DQVSSVATERVLATVVDCARRHKVLTVGDSRARAGALKGFDVVVPNDREAGIGAGIEV
                     VDEASLDAAAERLLQIAAGALITRGPAGIRVYRPQAEPVDVPIAIAPDAVVDVTGAGD
                     TVTAAAALTLVAGGSLEDAAVLGNAAAGVAVVQPGVVTVSNAELLDALASSGAPSKLK
                     TVDSLKRILEELHRDGKRVVWTNGCFDLIHVGHITYLQRASALGDVLVVGLNSDTSVK
                     QNKGPDRPIIPQENRAVVLAAFECVDYIVIFDEPTPMRLLEYLKPDVYAKGGDYSIDT
                     IVQEERRLVEGYGGEIAIISGVEGQSTTQIVSRMTGH"
     gene            2089..3069
                     /locus_tag="PKW60_12135"
     CDS             2089..3069
                     /locus_tag="PKW60_12135"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK00226.1"
                     /translation="MELKGEAVAIPPQQLMPVPVACLREETVTSFSLYIQTQGGQPPV
                     LYRDKHLPFTDNDRRRLEEHNIKYLFVDTREEKAYLRYLEKNLGAILGDPELPLEHKT
                     DVVYVSGRFLMREVFADPRAGDTVNRSKAYVQLTYEFLSQEKTAFAQLLRITSYDYYT
                     YTHSINVFVFSITLAQRAGAFSPQELKDFGTGALLHDIGKSLIDPSIVNCKGKLTLEQ
                     WEEMKRHPLLGCRILSGHNGISAAALDIVRYHHEKVNGSGYPEQRDAKTLSPLIRICT
                     IADIFDALTTRRSYKDAVTTFEALRIMKDEMADQLDADLLQTFIKMMGSP"
     gene            complement(3084..>3352)
                     /locus_tag="PKW60_12140"
     CDS             complement(3084..>3352)
                     /locus_tag="PKW60_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HPK00227.1"
                     /translation="YLTTEEKVAVCQMSLRSGAAFVKTSTGFGDSGATIQDVTLMYEV
                     VGKQMGIKAAGGVRTYGDAIALIEAGATRIGTSTGPSILEAAPR"
BASE COUNT          681 a          931 c         1062 g          678 t
ORIGIN      
        1 gagctccggg acgaggtgtc cgagggggaa cgctggcagt gttacgcgga agtgcgctgt
       61 gatgcgaagg catcctcggg cgtggcgctg gcggttggct tgtatgacgc gaccggcaaa
      121 aaacacatag tgcaacgccg tgtgccgatc gccgacgtgg ctggcgcgga ctacaaaaca
      181 atcgatcttg gtacgcatgc gctcgcgccg gggatgtatt tctgggcggc gcccgtgaac
      241 aaccccgacg aggtcgacgc gatttatgtg gatcggttct atctgctgcg caaacgttga
      301 gagtcggcgg cgcagacgtc gaaaatcctg cgttgtgttt gaattcgagt tgtgctgtaa
      361 tctggcgttt caatgatggc atgaaaggcc gtagcagccg gggaatactg gggagctgag
      421 atcgcatgag ctacgcggag ttggtggaac agttcaaaaa agcgcgggtt atggtggtgg
      481 gggacatcta cctggacgag aatgtctatg gcgccgtgac aggcgtcagt ctcgaagccc
      541 caatccccgt gtatgaagtg catgagaagc ggcacaatcc cggtgcggcg ggaaatgccg
      601 cctgcaatgt ggcctcactg ggcgcgacga cctacatggt gggttacgtt ggcgatgatg
      661 tgaatgcggc catcgtgcgc cgcgaatttg cggtccgcaa cgtcaatacg gactacgtgg
      721 tgactgatcc gacgcgcccg acgaacacct acggcaaact gcgcgcgggc gggttcaata
      781 tcccgagcca ggagatcctg cggaccgaca cgccgtctcc cacgttcatc tcgggcgcgg
      841 tcgaagatca ggtcgtcgcc catattgaaa aactggcgcc agaggtggat gccatcgtgg
      901 ttgttgacca ggtgtcttcc gtggcgacgg aacgcgtgct ggcaaccgtg gtggactgcg
      961 cccgccggca caaggtgctt acggtgggcg attcgcgtgc ccgcgcgggc gccttgaagg
     1021 gatttgacgt tgtggtgccg aacgatcgcg aggcaggcat cggcgccggc attgaggtgg
     1081 tggatgaggc gagcctggat gcggccgccg aacggctgct gcaaatcgcg gcgggcgccc
     1141 tgatcacacg cggacctgcg ggcattcgcg tgtaccggcc tcaggcggag ccggtcgatg
     1201 tcccgatcgc cattgcgccg gatgccgtgg tggacgtgac gggcgccggc gacacggtga
     1261 cggcggctgc ggcgctcacg ctggtggcgg gtggttcgct cgaagacgca gccgtactgg
     1321 gcaatgcggc ggcaggtgtt gccgtcgtgc agccgggcgt cgtcacggta tcaaatgcgg
     1381 aactcctcga tgcactggcg tcgtcgggcg cgccctcgaa gctcaagacc gtggattcgc
     1441 tcaaacgcat tctcgaagag ctgcaccggg acggaaagcg cgtcgtctgg acgaacggct
     1501 gctttgatct tatccatgtt ggacacatca cgtaccttca gcgcgcgtcg gcgctgggcg
     1561 atgtgctggt ggtcggcctc aacagcgata cgtccgtaaa acagaacaag ggccccgatc
     1621 gccccatcat tccccaggag aatcgagccg ttgttctggc ggcctttgag tgcgtggatt
     1681 acattgtcat ttttgatgaa cccaccccaa tgcggctgct ggaatacctc aagccggatg
     1741 tctacgccaa ggggggggat tactcgattg acaccatcgt tcaagaagag cgacgcttgg
     1801 tcgaaggata tggcggagag atcgccatca tctcgggagt cgagggccag tccaccacgc
     1861 aaatcgtcag ccgaatgacg gggcactagg catcgaagcg ctgctgcggc aaagagctat
     1921 aaccagttta ttcaaaatgc tttagggttg taaacctcga aaggcatcta tgaaatcccg
     1981 cgatttgcgc gggatttgcg gcagacaggc tgtttgcgca gttttgtcaa aaatgcctga
     2041 ttcgagtata atttattgtg acctgaggtt ctcaacgggg gattccctat ggaactcaaa
     2101 ggcgaggcag tggcgatccc gcctcagcaa ctcatgcccg tgcccgtagc ctgcctgagg
     2161 gaagaaacgg tcacgagctt cagcctgtat attcagacgc agggcgggca gccgcctgtg
     2221 ctttaccggg acaagcatct gccttttacg gacaacgacc gccgccgcct tgaagagcac
     2281 aacatcaaat acttgtttgt ggatacccgc gaagaaaagg cgtacctgcg gtatctcgag
     2341 aagaatctcg gggccattct gggcgatccc gaactccccc tcgaacacaa gacggatgtg
     2401 gtatacgtgt cgggccgttt cctcatgcgg gaagtcttcg ccgatccgcg cgcaggagac
     2461 acggttaatc gaagcaaggc ctatgtgcag ttgacctatg agttcctctc ccaggagaaa
     2521 accgcctttg ctcaactcct tcgcatcact tcatatgact attacaccta tacccacagc
     2581 atcaacgtct ttgtgttcag cattaccctg gcgcagcgcg cgggtgcgtt ttcgccacag
     2641 gaattgaagg acttcggcac gggcgcgttg ctgcacgaca tcgggaaaag cctcatcgac
     2701 ccgtccatcg tgaactgtaa aggcaagctc acgctggaac agtgggagga gatgaagcgc
     2761 catcccctgc tggggtgccg gattctgtcg gggcacaacg ggattagtgc cgccgcgctg
     2821 gatattgtcc gctaccacca cgaaaaggtg aacggttccg ggtatcccga gcagcgcgac
     2881 gcgaagacgc tctctcctct gattcgcatc tgcaccattg cagacatttt tgatgcgctg
     2941 acgacccggc gctcgtacaa ggacgccgtg acgacgtttg aagcgttgag gatcatgaaa
     3001 gatgagatgg cggatcagct tgatgctgat ttattgcaga cgttcatcaa gatgatgggt
     3061 tcaccctgac ccgccgcgcg cgcttagcgg ggggcagctt cgagtatgct gggcccggtg
     3121 ctggtgccga tgcgggttgc acccgcctcg atcagcgcga tggcgtcacc ataggtccgc
     3181 accccgcccg cggctttgat gcccatctgc ttcccgacaa cttcgtacat gagggtcaca
     3241 tcctgaatgg tggcgccgct gtcgccgaat cccgtggagg tctttacaaa cgcagcgccc
     3301 gaacgcaggc tcatctggca tacggccacc ttttcttctg tggtcaggta gc
//
LOCUS       DAOPBQ010000416         3351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22707_length_3351_cov_4.187500, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000416 DAOPBQ010000000
VERSION     DAOPBQ010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22707_length_3351_cov_4.187500"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..891
                     /locus_tag="PKW60_12145"
     CDS             301..891
                     /locus_tag="PKW60_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPK00228.1"
                     /translation="MLTLSNYCTELSRVLARIDEAAFDAAVKAIREVHAADKVIYVCG
                     NGGSAATASHMVNDLVKAPADAAGCRPFRAFSLTDCVPLMMAFANDVDYAQALALQLE
                     ACGRPGDLLVAFSGSGNSPNVLEAVKTAKAKGLSVLGFTGFQGGKLGPLCDVHVNVPC
                     DSMAQVEDAHLIIEHALVEVLKESFGGKSSVTCGPA"
     gene            919..1890
                     /locus_tag="PKW60_12150"
     CDS             919..1890
                     /locus_tag="PKW60_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPK00229.1"
                     /translation="MESLVLGIEIGGTKLQAVLGTAAGEILAQERGRAPAGEGAQAIL
                     AWFEEAVPRLLAEASSRGRTVTGMGVGFGGPVESATGTVLVSHQVGGWDGLPLKTWFE
                     ERFELPAAVVNDSNAAGWAEYCLGAGRGTRHFCYMNIGSGIGGALILDGKLHDGQGLG
                     AAEIGHCHVPDWTAPESGASEKLEKLCSGWSIERRFRTTPDLAPGSVLHRLCEGDRER
                     ITCAMVAEAARAGDPVCLEALDHVAQSVGLALANLITLVHPERVALGGGVALMGEVLL
                     EPLRRHVDARVFEQFRGRYDIVPCALGESVVVAGALLLAPRPPGKVM"
     gene            1942..2676
                     /locus_tag="PKW60_12155"
     CDS             1942..2676
                     /locus_tag="PKW60_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPK00230.1"
                     /translation="MTGERFAAGAQSYSFRKFDLAGAIACLKQLGADTMEFCAGHFPP
                     DAEDAGFAEVRRLLREQHVQVPCFGVENFTADAAANRKKFEFARALGAQVLTADPAPE
                     ALDSLDALVDEFQIKIAIHNHGPGARYDKVEDTLRAVQGRHPFLGACLDTGHCLRTNE
                     LPQEVIKRLGDRLISLHLKDWIIGGKEQILGEGDLDLAAVAGALLETGFTGPIVMEYE
                     ESPENPVPDMKTGLANWRAAVQAAGA"
     gene            complement(2685..>3351)
                     /locus_tag="PKW60_12160"
     CDS             complement(2685..>3351)
                     /locus_tag="PKW60_12160"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HPK00231.1"
                     /translation="GSSVNPQRQREAHAHSVNFDPSGRFLFAADLGTDQIMIYRFDEA
                     HGTLEPNDPASVQLAPGAGPRHFALHPSGKFAYVINELDNTITAFAFDPATGRLDAVH
                     SVSTLPEEFTGESYTAEIKVHPSGRFVYGSNRGHDSIAIFAVDPASGVLTPAGWASTQ
                     GKAPRHFNMEPAGQFLIAANQDSDSLVVFRIDPASGALNATGQAVIVPTPVCVVFRPV
                     PAN"
BASE COUNT          585 a          950 c         1209 g          607 t
ORIGIN      
        1 cgatggatgg gtcgttccgc agacagaacc cggtgaaata cagcctcaat caaatccttc
       61 atttcgcgga cgtaacgcgc accatcgagg acgtcgtgct tcccgcgaag acggtatgac
      121 cccctaaaac gccgccttct tccgcaattc cttctgccag tatgcaaact ggcccggtgc
      181 gtcttgaaac cgttggcgcg cgcctcttag gatagacttg attcagcccc gggtgcgggc
      241 aggttcccac gacactgcag cttgtctgcc gggttgccgg cggcatcgga aaggaaatgc
      301 atgctcacgc tttcgaatta ctgtacggag ctcagccgcg tcctggcccg gattgacgag
      361 gccgcgtttg atgcggcagt caaggccatc cgggaggttc atgccgcgga caaggtgatc
      421 tacgtgtgcg ggaacggcgg cagcgccgcc accgcgtcgc atatggtcaa cgatctcgtg
      481 aaggcgccgg cggacgccgc gggatgccgg ccgttccgcg cgttttcgct gacggattgc
      541 gtgccgttga tgatggcgtt cgccaacgat gtggattacg cgcaggcgct ggcgctgcag
      601 ctcgaagcct gcggccggcc gggcgatctg ctggtggcgt tttccggcag cggcaattcg
      661 cccaacgtgc tcgaggcggt gaagacggca aaggcaaaag gcctttcggt attgggattc
      721 acggggttcc agggtgggaa gctgggtccc ctgtgcgatg tccacgtcaa tgtcccctgc
      781 gattccatgg cgcaggtcga agacgcccac ctgattatcg agcatgcgct ggtcgaggtc
      841 ctgaaagaat cgttcggcgg gaagtcgagc gtgacctgcg gtcccgcatg acagacctgg
      901 gcgaaggttg atttgattat ggaaagcctg gtgcttggca ttgaaatcgg gggcacgaaa
      961 ctccaggccg tgctcggcac ggccgcgggg gagatcctgg cgcaggagcg tggccgggcg
     1021 ccggcggggg aaggggcgca ggccatcctc gcctggtttg aagaagccgt gccccggctg
     1081 ctcgcagagg cctcgtcacg cggccggacc gtgacgggca tgggtgtagg gttcggcggc
     1141 ccggtcgagt cggccaccgg cacggtcctg gtctcccacc aggtgggcgg ctgggacgga
     1201 ctgccgctca aaacgtggtt cgaagagcgc ttcgaactgc cggccgccgt ggttaacgac
     1261 agcaatgcag cggggtgggc ggagtattgc ctgggtgcgg gccgggggac ccgccatttt
     1321 tgttacatga acatcggcag cggtatcggc ggtgcgctga tacttgacgg aaaactgcac
     1381 gacggccagg gcctgggcgc cgcggagatc ggccattgcc atgtgccgga ctggaccgcc
     1441 ccggaatctg gcgcatcgga gaagcttgag aagctgtgct ccggctggtc gatagagcgg
     1501 cggtttcgga ccacgccaga cctcgcgccg ggttcggtgc tgcaccgtct ctgcgaaggc
     1561 gatcgggagc ggatcacctg tgcgatggtg gcggaagcgg cccgtgcggg cgatccggta
     1621 tgtctggaag cgctcgacca cgtcgcgcaa tcggtcggat tggcactggc caatctgatc
     1681 acgctggttc atccggagcg agtggcgctt ggcggcggcg tggcgctgat gggcgaggtg
     1741 ttgctggagc ctttgcgccg gcatgtcgat gcgcgggtgt ttgaacagtt tcgtggccgc
     1801 tacgacatcg tgccgtgcgc gctgggcgaa tccgtggtgg tcgcgggtgc gctgttgctg
     1861 gcgccccgtc cgcccggcaa ggtcatgtaa caggcgcgtg tggcttgctc actctaaccc
     1921 acgaaaaagg aaggggatgc catgacaggg gaacggttcg cagcaggggc gcagagttac
     1981 agctttcgca aattcgatct ggccggggcc atcgcgtgtt tgaagcaatt gggcgcggat
     2041 acgatggaat tctgcgcggg gcattttccg ccggacgcgg aagatgccgg gtttgcggaa
     2101 gtccgccgct tgttgaggga gcaacacgtg caggtgccgt gttttggtgt ggaaaacttc
     2161 acggctgacg cagcggcgaa ccggaagaag ttcgagttcg cccgggcgct cggcgcgcag
     2221 gtgctgaccg cggatccggc gccggaggcg ctggacagcc tggacgcgct ggtcgacgag
     2281 ttccagataa aaattgcaat tcacaatcac ggtccggggg cccgctacga caaggtggaa
     2341 gacacgttgc gagccgtgca ggggcggcat ccgttcctgg gcgcctgcct ggataccggg
     2401 cactgtctgc gcacaaacga attgccgcag gaagtgatca agcgtctcgg cgatcggctt
     2461 atctcgctgc atttgaagga ctggatcatc gggggaaaag agcagatcct gggcgagggc
     2521 gatctggacc tggccgccgt agcaggcgct ctgctggaaa ccggcttcac cggccccatc
     2581 gtgatggagt acgaggagag ccccgagaat cccgtgccgg acatgaaaac gggcctggca
     2641 aactggcgcg cggccgtgca ggccgccggc gcctgagccg ccgcctaatt cgccggcact
     2701 ggcctgaaca cgacgcacac gggcgtcggc acgataacgg cctgtccggt ggcgttgagc
     2761 gcgccggatg ccggatcgat ccggaacacg accaggctgt ccgagtcctg gttggcggcg
     2821 atcagaaatt gtcccgcggg ctccatgttg aaatggcgcg gcgccttgcc ctgagtggag
     2881 gcccatccgg cgggcgtgag cacgccggat gcggggtcaa cggcgaatat ggcgatgctg
     2941 tcgtgcccac ggttcgagcc gtagacgaag cggccggaag ggtggacctt gatctccgcg
     3001 gtgtagcttt ctccggtgaa ttcctcgggc agcgtggaga cggagtgaac tgcgtcgagg
     3061 cggcccgtgg cggggtcgaa agcgaatgcg gtgatggtgt tgtcgagctc gttgatcacg
     3121 tacgcgaact tgccggaggg atggagcgcg aaatggcgcg ggccggcgcc cggagccagc
     3181 tgtacggaag cgggatcgtt cggttcaagc gtgccgtgcg cttcgtcgaa gcggtagatc
     3241 atgatttgat ctgtgccgag atcggcggcg aacaagaagc ggcccgaggg gtcgaaattc
     3301 accgagtggg cgtgggcttc acgctgccgc tgcggattga cactcgaccc c
//
LOCUS       DAOPBQ010000417         3344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22773_length_3344_cov_3.390088, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000417 DAOPBQ010000000
VERSION     DAOPBQ010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22773_length_3344_cov_3.390088"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            353..1483
                     /locus_tag="PKW60_12165"
     CDS             353..1483
                     /locus_tag="PKW60_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00232.1"
                     /translation="MTRCACIVVLFVSSAVCAFCAEEGGVGEPVEVEVESGAEVYLLG
                     GDERPADNIYGEQPYSDATGRRIAIRYYPTKDRPGGINIFDLEDGSNHEILSGKPPFP
                     AFHAWGEWLYFAQMVDGENWLRRCNFLSLAIENVAKLPPERGAYSYGTVSPDHRHFAV
                     SVRPPEGGPSQVHWLDLSTNEWQVLLDKPGYHAKHEQFSRDGRNRVLIQLNQMPDVRV
                     VLLSELELGGPEQPFPADQPYTLRPTGHEAWIGDTSEIFFSTALDKDLNGNVWRGKVG
                     DEKPTLVYRGKTFMHVSVSRDGKFWIGDVNEEGIPIYIGSFESGRCERVCFSRTELGK
                     EQWTHAHPYLTADNRWLIFGSQRNGHPQAYGAKLKESWLDSL"
     gene            complement(1523..2308)
                     /locus_tag="PKW60_12170"
     CDS             complement(1523..2308)
                     /locus_tag="PKW60_12170"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPK00233.1"
                     /translation="MTDLSRIDISAARTARYVEIRRWHAGADWHPRPHAHPFCELIIA
                     LSGAARVLVEGTETICEVGEVLFYKPGTRHSEWQHGSALYEFYCLHFEWPDCPQEFQH
                     HIYDYDGRIRELARWLYVDRLYDTRGIQAYRDILTQSLVSELTRLSGNPRTSSIDLIR
                     KFVRENLGEPLNLDVLAEVCSMNKFHLARQFRAATGLTLMKYVRSIRLDVAFQLIVET
                     ALPLREIAAQTGFSSEYHLSRLIKSRYGRGTRELRRQFGSVMK"
     gene            2510..>3344
                     /locus_tag="PKW60_12175"
     CDS             2510..>3344
                     /locus_tag="PKW60_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK00234.1"
                     /translation="MSKKGFTLIELLVVIAIIGILAAILLPALARAREAARRASCQNN
                     LKQWGLVCKMFANESKGEIWPRVLNQDYSRDANGRRRVGRVRGGIWGPSVYPEYVTDL
                     NLWICPSAVNAGTLADRWKCPGGAYCQSDCVADPAYPGFDIAKMGNDEQHSYYYYGYI
                     TDTDGAFATMQVTLRPYATLVCTNAGGSGDYTAGTDYASHTAHPARAAVDSALSSDYD
                     PSKSGMSWAQIQSTIDAQIAGSGYTPPTPIVAQGTAGSTTLTKLREGAERFLITDINN
                     PA"
BASE COUNT          747 a          895 c         1002 g          700 t
ORIGIN      
        1 cctagtgtgg gagttgggaa tgcctggggg tcaagaggcc gctggttcaa atccagtcgc
       61 cccgaccatt gctatcaagg acttacgcga ttaacgcgta ggtccttttt tgttctgtgg
      121 cggcgcttgc agtggggcag ggcgggcgca gtcaggggag gcgttgtgtt gatcatgcgg
      181 ggagcagcat ttacactcgc ttatagagaa agtcggcgct ggcattccgc cccatggccg
      241 cgtgatcgct ggccacgtga actcgatgaa agggaatgcc cgtcggtggt agcgtttcga
      301 taagactttt ccggccagaa gctcagagca gctaaaacct tgagaggtag ccatgactcg
      361 ctgtgcatgt atcgtcgttt tgtttgtgtc ttcagccgtc tgtgcgtttt gcgccgaaga
      421 aggtggggtt ggcgaacccg ttgaggtgga agtggagagt ggggcggagg tttatcttct
      481 gggaggcgac gagcgtccgg ccgacaacat ttatggcgag cagccctata gtgacgccac
      541 ggggcggcga attgccattc gctattatcc cacgaaggat cggcccggcg gcatcaacat
      601 tttcgacctc gaagatggtt ccaatcatga gatcttgtct ggcaaaccgc cttttccagc
      661 tttccacgcc tggggcgagt ggctgtattt tgctcagatg gtcgatgggg agaactggct
      721 gcgccggtgt aatttcttga gcctggcgat tgagaatgtg gcgaagctgc ctccagaacg
      781 gggcgcatac agctatggca ccgtttctcc agatcaccgg cactttgccg tgagcgtgcg
      841 cccgcccgaa ggtggcccat ctcaggtgca ctggctggac ttgagcacca acgagtggca
      901 agtccttctg gacaagcccg gataccacgc caaacacgag caattctcgc gcgacgggcg
      961 taaccgggta ctgattcaat tgaaccagat gccggatgtg cgggtcgttt tgctcagcga
     1021 actggaactc ggcggtccgg agcagccgtt cccggcagat cagccctaca cgttacgtcc
     1081 taccggacac gaagcctgga ttggcgacac cagcgagatc tttttctcga ccgcactgga
     1141 taaagacctg aacggcaatg tctggcgcgg caaggttggc gacgagaagc caacgctcgt
     1201 ttacaggggg aaaacgttca tgcatgtcag cgtttcccgc gacggcaagt tctggatcgg
     1261 ggatgtcaat gaggaaggca tccccattta tattggaagc ttcgaatcgg gacgctgcga
     1321 gcgggtgtgt ttcagccgca cggaacttgg caaagagcag tggacacacg ctcaccccta
     1381 cctgacagca gataaccgat ggctcatctt tgggtcgcag cgcaacgggc atccccaggc
     1441 ctacggcgcg aaattaaagg aaagctggct cgacagcctg taattggcgc ttgaatgggg
     1501 ctggggcaat tggagaggtg ctctatttca ttacgcttcc gaactgccgc cgaagttcgc
     1561 gcgtcccacg gccatacctg cttttaatca gccgggaaag gtgatattcg ctggaaaatc
     1621 cggtttgggc agctatctcc cgcaacggaa gtgcggtttc gacaatcaat tggaacgcca
     1681 catccaggcg gatcgagcgc acatatttca tgagcgtaag gcccgtagcg gcgcgaaatt
     1741 ggcgcgccag gtggaacttg ttcatgctac agacttcagc caacacatcc agattcagcg
     1801 gctcgccgag attttcccgc acaaacttgc gaatcaggtc gatgctgctc gtccgcggat
     1861 tgcctgagag gcgggttaat tcagaaacaa gcgattgcgt caatatatcg cgatacgcct
     1921 gaatgccgcg ggtatcatag agcctgtcta cgtagagcca tcgcgccagt tcacgtatcc
     1981 ggccgtcata atcatagata tgatgctgaa attcttgagg acaatccggc cattcgaagt
     2041 ggaggcaata gaactcgtag agcgcgctgc cgtgctgcca ttctgaatgc cgggtacctg
     2101 gcttataaaa aaggacttcc cccacctcgc agatggtctc ggtgccttcg acaagcaccc
     2161 gtgccgcacc ggagagagcg attatgagtt cgcagaacgg atgtgcatgc ggacgcggat
     2221 gccaatcggc cccggcatgc caacggcgaa tctcaacata ccgcgcagta cgcgctgcag
     2281 agatgtcaat tctagataaa tccgtcaatt atcgctattg cctttctcac atttcgttgc
     2341 tagtgtgaaa accgcatacg ggaattatcc cgcatgtccg cggtctgatc aaggggtagt
     2401 agcggcataa gagccaggaa agcgaggtgg aagagactac ttaagggcgt gtactaagag
     2461 gcaaagacat agagatccct acgtcaactg cagggaaaag gaagatatca tgagcaaaaa
     2521 gggattcacg ctgattgaat tgcttgtcgt aattgcgatt attgggattc tggcggcgat
     2581 cctgctcccg gcacttgccc gggcccgcga agccgcgcgg cgggccagct gtcagaataa
     2641 tctcaagcag tggggtctgg tttgcaagat gtttgccaac gaaagcaagg gggagatctg
     2701 gccgcgagtc ctgaatcaag actactcgcg tgatgcaaac ggccgcagac gcgtcggccg
     2761 cgtgcgcggc ggcatctggg gcccttcagt atatcctgag tacgtgacgg atctgaactt
     2821 gtggatctgc ccctcagcgg tgaatgccgg cacactcgcc gataggtgga agtgcccggg
     2881 tggtgcttac tgccagagcg actgcgtggc cgatcccgcc tatccgggat ttgacatcgc
     2941 caaaatgggc aacgacgagc agcacagcta ctactattat gggtacatca ccgatactga
     3001 cggagcgttt gccactatgc aggtgacgct gcggccttat gccaccctgg tctgcaccaa
     3061 cgctggcggc agtggagact atacggcagg aaccgattat gcgtcgcaca cggcgcaccc
     3121 ggcccgggct gcggtggact cagcgctttc gtccgactac gatccaagca agtcgggtat
     3181 gtcatgggcc caaatacagt ccaccatcga cgctcaaatc gcgggcagcg gttatacacc
     3241 gccaacgcca attgtggcac aggggacagc cgggagcacc acgctcacga aattgcgtga
     3301 aggtgccgag cgtttcctga tcaccgacat caacaaccct gcgg
//
LOCUS       DAOPBQ010000418         3341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22797_length_3341_cov_4.298235, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000418 DAOPBQ010000000
VERSION     DAOPBQ010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22797_length_3341_cov_4.298235"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..1033
                     /locus_tag="PKW60_12180"
     CDS             278..1033
                     /locus_tag="PKW60_12180"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPK00235.1"
                     /translation="MSDGDLGSFERIAQYYEALANAPKRLEREGPFLRDLLSRAPGKR
                     VLDVACGTGLHALWFAEQNAEVTAVDLSEGMIAYARTHRAHERIEYQVCDMRKPPHGT
                     WDLILSLGNSLCLLNAEDELRQVFDSIQERLSPGGQFVFQVLNYERKEALLPRHRIET
                     AGLPGGQLTAIKNLVPAGNRTLLALAFFVENASGRFETATDTSVLRNWTKDDLLSLAL
                     ENALEPAGLFGRFSGEPFMPDDSPDLIVVLRKA"
     gene            1130..2218
                     /locus_tag="PKW60_12185"
     CDS             1130..2218
                     /locus_tag="PKW60_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK00236.1"
                     /translation="MTLTGAERIANILRRKPVDRVGLFEHFWGDTHKKWTREGRLKEG
                     ELLADHFGFDIEMGGWFNMTADLDFEPVVVEETRDTVLTRDGNGALLRRHKLHDATPE
                     HVDFLVKDRASWDAHAKPLLKPDRRRTDFVAYREARQHAAGAQRFFCWGGTNVFELMH
                     PVCGHEYMLLGMAADPDWVKDMVGTYADLVIEMMEMLFGEEGWPDGIWFYEDMGFKGR
                     PFVSPGMYREIIQPGHRKTIDFVKSRGIPVIMHSCGYVEPLVPGMLDAGIDCLQVIEV
                     KAGMDLVKLYRDFGDRLSFMGGIDVRVLYSNDTSQIEAELQAKIPVVKSNYGYVLHSD
                     HSIPDQVDYESYRYFTQKGLELGRYDGK"
     gene            2250..2924
                     /locus_tag="PKW60_12190"
     CDS             2250..2924
                     /locus_tag="PKW60_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144592.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPK00237.1"
                     /translation="MSEELLQQLAVCVERGKVNQASPYPPDMKGQNGADELAKQALEQ
                     GISPNDVLEKALIIGMGNIGKKFSANQVFVPDVLMAAKAMSAAMAHLKPFLDSGQVKT
                     KGTFVIGTVQGDLHDIGKNLVCMVMEGGGWRVVDLGVDVPPEKFLAAVQANPGAAVGL
                     SALLTTTMVNMEKTVKLIKDEVSGTKVVVGGAPLTQEFAAKIGADAYSPDPQGALDYL
                     NATVAA"
     gene            3137..>3341
                     /locus_tag="PKW60_12195"
     CDS             3137..>3341
                     /locus_tag="PKW60_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00238.1"
                     /translation="MYVGEGIRYVVCSGLLLAVACATACAEDAGDIYLGDAWEELAPQ
                     HTQAWGSLGLNTAAKPPDGRAATP"
BASE COUNT          686 a          910 c         1068 g          677 t
ORIGIN      
        1 ggtcttgatc tcgttttcga tctcctcggg cgtgggcggc ggcggagggg gtggtggcgg
       61 gggcggcggc ggcggcggcg gtggctcggc tttgcagccc gcgaacgcga tcaagcatac
      121 tccaatcaca aaggcaacca gaaaggctcg tttcatgcgt aattctccat actcggtgcg
      181 tatgttcagg cagtaaaccg attatacttg acaatggcgg aaagtacaaa tcgtgcaacc
      241 acttgggaga tgtaaatcct gtcaggagga agtctgtatg agtgacgggg atctcggttc
      301 gttcgagcgg atagcccaat attacgaggc gctggccaat gcgcccaaac gtctggagcg
      361 cgaaggccca ttcctgcgcg acctcttgtc ccgtgcgccg ggcaaacgcg tgcttgatgt
      421 tgcgtgtgga actggtctgc atgccttgtg gtttgcggaa cagaacgcgg aggtgaccgc
      481 ggtagacttg agcgagggca tgattgccta cgcgcgaaca catcgcgcgc acgagcggat
      541 tgagtaccag gtttgcgata tgcggaagcc gccccacggc acgtgggatc tgatcctcag
      601 cctcggcaac tctctttgcc tcctgaatgc cgaagacgag cttcggcagg tgtttgacag
      661 catccaggag cgcctttccc caggcggaca gtttgtcttt caggtactca actacgagcg
      721 aaaagaagcc ttgctgccgc ggcaccgcat tgaaaccgcc ggcctccccg gaggccagtt
      781 gaccgcgatc aaaaacctcg tgccggcggg caatcgtacc ctcctggccc tggccttttt
      841 tgtcgagaac gcgtcaggcc gcttcgagac cgccacagat acgtcggtac tgcgcaactg
      901 gaccaaagat gatctgctct cgcttgccct cgagaatgcg cttgagcccg cgggactgtt
      961 tgggcgattt tccggggagc catttatgcc cgacgactcg ccggatctca tcgtcgtgct
     1021 tcgaaaggca taggcccggc aactgaccgg gatcgctttg ctttcaagcg catttttgtg
     1081 ctatgacacg cgaaatttcc ccgcagcagc acgacggaga cgtgtcctga tgacgttgac
     1141 gggcgccgaa cgcatcgcca atatcctccg gcggaagccg gtggaccggg tagggctgtt
     1201 cgagcacttc tggggcgata cgcacaaaaa atggacgcgc gaggggcgtc tcaaggaagg
     1261 ggagctgctc gcggaccatt tcggcttcga cattgagatg ggcggctggt tcaatatgac
     1321 cgcggacctg gatttcgagc ccgtcgtggt cgaagagaca agggataccg tgctgacgcg
     1381 cgacggcaac ggcgcgcttt tgcgccggca taaactccac gatgcgacgc cggagcatgt
     1441 ggatttcctc gtgaaagacc gggcaagctg ggacgcgcac gccaagccgc tgttgaagcc
     1501 cgaccggcgg cgcaccgatt tcgttgccta tcgcgaggcg cggcagcatg ccgccggggc
     1561 gcagcgcttc ttttgctggg gcgggaccaa tgtgtttgaa ctcatgcatc ccgtgtgcgg
     1621 ccatgagtat atgctgctgg gcatggcagc cgacccggat tgggtgaagg acatggtcgg
     1681 cacatacgcg gatctggtca tcgagatgat ggagatgctg tttggcgaag aaggctggcc
     1741 agacggcatc tggttctacg aagatatggg attcaagggc cggccctttg tgtcgcccgg
     1801 gatgtaccgg gagatcattc agcccgggca ccgcaagacc atcgatttcg tcaaaagccg
     1861 gggcataccc gtcatcatgc attcgtgcgg ctatgttgag ccgctggtgc cgggaatgct
     1921 ggacgcgggg atcgattgcc tgcaggtgat tgaagtcaag gcagggatgg atcttgtaaa
     1981 gctttacaga gatttcggcg atcggctatc cttcatgggg ggaattgatg ttcgcgttct
     2041 atactcgaac gatacctcac agatcgaggc agaattgcag gcgaaaattc ccgtggtgaa
     2101 gagtaattac gggtatgtgc ttcactcgga tcactcaata cccgaccagg tcgactacga
     2161 gagctatcgg tatttcactc aaaaaggttt ggagctgggt cggtatgacg gcaagtaagg
     2221 cgaaacggct tcgctaagag gaggcggaca tgtccgagga actgttacag caactggcgg
     2281 tttgtgtcga gcgcggcaaa gtcaaccagg cctcacccta tccgcccgac atgaagggtc
     2341 agaacggcgc cgatgaactg gcgaagcagg ccctggagca aggcatttcg ccaaatgatg
     2401 tgctcgagaa agccctcatc attggcatgg gaaatatcgg gaagaaattc agcgcgaacc
     2461 aggtcttcgt gcccgatgtg ctcatggcag ccaaagccat gagcgccgcc atggcacatc
     2521 tgaaaccgtt tctggattcc ggacaggtca agaccaaagg cacgtttgtc atcggaaccg
     2581 tacagggcga tctgcatgat atcggaaaaa acctggtctg catggtcatg gaaggcggcg
     2641 gctggagggt cgtggacctc ggcgtggatg tgccgcccga aaaattcctc gcggcggtgc
     2701 aggccaatcc gggcgcagcg gtcggcctga gtgccctcct gaccaccacg atggtcaaca
     2761 tggaaaaaac agtcaagctg atcaaggacg aggtgtcggg gacgaaagtg gtcgttggcg
     2821 gcgctccgtt gacgcaggaa ttcgcggcaa agatcggcgc ggatgcctat tcccccgatc
     2881 cccagggcgc cctggactac ctgaatgcca ccgtggccgc ctgagctcgg ctggcgcggt
     2941 tctttttttg gcagacgcgg ccctttcaac agcgaaaggg ccgcgttgcg ttctgtctgg
     3001 aaggcgaaac cgcccgcatt ccattgaaca ggcgctgatt tgattccggg gctgtttctc
     3061 cagatacttg atatggatcg cgcacgcctg cgcgacgcga tgaatggctc cggcgggaac
     3121 tattggagac cggttcatgt acgttggcga aggtattcgt tatgtcgtgt gttcagggtt
     3181 gctgctggcc gttgcatgcg ccacggcctg cgctgaggat gccggcgata tctacctggg
     3241 cgatgcctgg gaggaactcg cgccgcaaca tacccaggct tggggttccc tgggcctcaa
     3301 cacggccgct aagccgccgg acggccgcgc tgcgacgcct t
//
LOCUS       DAOPBQ010000419         3319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_22973_length_3319_cov_2.941483, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000419 DAOPBQ010000000
VERSION     DAOPBQ010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22973_length_3319_cov_2.941483"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PKW60_12200"
     CDS             complement(<1..1057)
                     /locus_tag="PKW60_12200"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPK00239.1"
                     /translation="MDQSLPVTRTRHPKDKPADPNKLGFGQIFTDHVFHMDYREGEGW
                     VDPRIEPYGPLVLDPATMAFHYGQTIFEGLKAYRFPDGSIRLFRPRLNFERINRSNDR
                     MVVPPVDPDFLVGALKQLLRLESDWVPSAPGTSLYIRPFIIATDPFLGVRPSRTYRLM
                     IILSPSGAYYPQGLNPVPIHVETKYVRAVRGGTGEAKTGGNYAASLRSQADAHARGFV
                     QVLWLDGVERKYIEEVGSMNVFFVLGGTLVTPSLCGSILPGITRRSVLELADHWGIPT
                     EERRLSMDEILEAGESGSLSEAFGSGTAAVISPIGELEYDGVRIRLHDGAIGPLSQRV
                     YDTLTGIQYGRLPDPLGW"
     gene            1245..3257
                     /gene="uvrB"
                     /locus_tag="PKW60_12205"
     CDS             1245..3257
                     /gene="uvrB"
                     /locus_tag="PKW60_12205"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPK00240.1"
                     /translation="MPNPADTRFRMKTDMVPCGDQPAAIDALVRGLDGGDAAQTLLGV
                     TGSGKTFTVANVIQRVERPTLVIAHNKTLAAQLCAEFKAFFPDNAVEYFVSYYDYYQP
                     EAYIPATDTYIEKDSSINEEIDKMRHSATRALLTRRDVLIVASVSCIYGIGSPETYGA
                     LRVELAPGEMISREDLLAGLVAMQYTRNDIDFHRGTFRVRGDIVDIFPAYEDAIAVRV
                     ELFGDEIEGLYEIDPLRSTVSRRLRRIAIYPGSHYATTADTMKRAIQGIEHELEECLK
                     GLHAEGKLLEAQRLEQRTRFDIEMLSEIGYCSGIENYSRHLDGRMPGEPPYTLLSYFP
                     PDSVVVIDESHITVSQIDGMYKGDRSRKDRLIEYGFRLPCARDNRPLKFREFWERVNQ
                     CIYVSATPGPYELEQSGGLIVEQVVRPTGLIDPAVEVRPATNQVDDLLDEVRKVVARN
                     ERVLVTTLTKRMAEDLTAYLADLRVRVRYMHSDVETLERIEIVRALRSGEFDVLVGIN
                     LLREGLDIPEVALVAVLDADKEGYLRSERSLIQTCGRAARNVNGRVLMYADTMTGSME
                     RAIGEMNRRRELQIAYNKKHRITPRSITKSIPKIMASIYERDYVTVPRAAEEQELYSS
                     SFDIQKTIRELRKKMKQAADTMEFEKAAEFRDRLLALEKRQIEDGL"
BASE COUNT          666 a         1036 c         1058 g          559 t
ORIGIN      
        1 tccagcccag gggatcgggc agccggccat actggatgcc cgtcagggtg tcatagacgc
       61 gctggctcag gggcccgatg gcgccgtcgt ggaggcggat gcgcacgccg tcatactcca
      121 gttccccgat gggggagatc acggcggcgg tgccgctgcc gaaggcctcg gacaggctgc
      181 cggattcgcc ggcttccagg atctcgtcca tggacagccg gcgttcctcc gtcgggatgc
      241 cccagtggtc ggccagctcc aggaccgagc ggcgggtaat gcccggcagg atgctcccgc
      301 agagggacgg agtcaccagg gttccgccga ggacgaagaa gacgttcatg gaaccgactt
      361 cctcgatgta ctttcgctcg acgccgtcga gccacaggac ctgcacgaat ccccgggcat
      421 gggcgtcggc ctgggatcgg aggctcgcgg catagttgcc gccggtcttg gcctcgccgg
      481 taccgccccg gacggcccgc acatatttcg tctccacatg gatgggcacc gggttcagcc
      541 cctgggggta ataggcgccg gatggggaga ggatgatcat gagccggtat gtccgggagg
      601 gacggacgcc gaggaagggg tcggtggcga taatgaacgg ccgaatatac agggaggttc
      661 cgggagcgga ggggacccag tcgctttcca gccgcagcag ctgcttcagg gccccgacca
      721 ggaaatccgg gtcgacgggg gggaccacca tccggtcgtt ggaccggttg atccgctcga
      781 agttcagtcg gggccggaaa aggcggatgg agccgtcggg gaagcgatag gccttcaggc
      841 cctcgaaaat ggtctgcccg tagtggaatg ccatggtggc gggatccagg accagggggc
      901 cgtagggttc gatccgcggg tcgacccagc cttcgccttc ccggtagtcc atgtggaaga
      961 cgtggtccgt aaagatctgt ccgaatccaa gcttgttcgg atccgcgggt ttgtccttcg
     1021 ggtgccgggt cctggtaacg ggcagcgact ggtccatggc ggtatccccc catcatgtga
     1081 tcgaaaccaa gatacaccat ccggcctcat gggggcaagg gccgcgggac cctgcggacc
     1141 gccccgtcgg ccgcgatacc cggcggtcgt ccgccgccgg catgatataa tggaagcctg
     1201 gcgacaaccg ggcgaatgcc cgccgcaacc tgaggaggcc tcccatgccc aacccggcgg
     1261 atacccgttt tcggatgaag acggacatgg ttccctgcgg cgaccagcct gcggccattg
     1321 acgcgctggt ccggggactg gatggcggag acgcggccca gaccctgctg ggcgtgaccg
     1381 gatccggcaa gacctttacc gtggccaacg tgatccagcg ggtggagcgc ccgaccctgg
     1441 tcatcgccca caacaaaacc ctggctgccc agttgtgcgc cgaattcaag gcctttttcc
     1501 cggacaacgc ggtggagtac ttcgtcagtt actacgatta ctatcagccg gaggcctata
     1561 tccccgccac cgacacctac atcgagaagg actcgtccat caacgaggag atcgacaaga
     1621 tgcgccactc agcgacgcgc gcgctgctca cccggcgcga cgtgctgatc gtggccagcg
     1681 tgtcgtgcat ttacgggatc gggtcgccgg agacctatgg cgcgctgcgt gtggaactcg
     1741 ccccaggcga gatgatctca cgcgaggact tgctggccgg gctcgtggcc atgcagtaca
     1801 cccgaaacga catcgatttt catcgaggca cgttccgggt gcggggcgac attgtcgaca
     1861 tctttccggc ctacgaagac gcgatcgccg tgcgcgttga actgtttggc gacgaaatcg
     1921 agggcctcta cgagatcgat ccgttgcgga gcacggtcag tcggcggctg cgccgcatcg
     1981 ccatttatcc gggttcgcat tacgccacga ctgccgacac catgaagcgg gcaatccagg
     2041 gcattgaaca cgagctcgaa gagtgcctga aagggctcca cgccgaggga aagctcctcg
     2101 aagcccagcg gctcgaacag cgcacccggt tcgacatcga aatgttgagc gagatcggtt
     2161 actgcagcgg catcgaaaat tactcgcgtc atctcgacgg ccgtatgccg ggcgagcccc
     2221 cctacaccct gctcagctat ttcccgccgg acagtgtcgt ggtcatcgat gagagccaca
     2281 tcaccgtatc tcagatcgac gggatgtata aaggcgaccg ctcgcgaaaa gacaggctga
     2341 tcgagtacgg cttccgtctg ccctgtgcgc gtgacaaccg ccccctcaaa ttccgcgaat
     2401 tctgggaaag agttaatcaa tgcatctacg tcagcgcaac gccgggcccc tatgaactgg
     2461 aacagagcgg tgggctgatt gttgagcagg tggttcgccc aaccgggctc atcgaccccg
     2521 ccgtcgaagt acggcccgca accaaccagg tcgatgacct gctggacgag gtgcgcaaag
     2581 tcgtcgcccg caacgaacgg gtactggtca ccacgctgac gaaacgcatg gcggaggatc
     2641 tgaccgccta cctcgccgac cttcgtgtgc gcgtacgcta catgcactcc gacgtggaga
     2701 cgctcgaacg cattgaaatt gtgcgggcgc tgcgcagcgg cgagttcgac gtgctcgtgg
     2761 gcatcaacct cctgcgcgaa ggcctcgaca tccccgaggt cgcgctggtg gccgtgctcg
     2821 atgccgacaa agaagggtac ctgcgctccg aacgcagcct gatccagacc tgcggccggg
     2881 ctgcgcgaaa cgtcaacggg cgcgtcctca tgtacgccga caccatgacc ggctccatgg
     2941 agcgcgctat cggcgaaatg aaccgacggc gcgaactcca gatcgcctac aacaaaaagc
     3001 accggatcac gccgcggtcg atcacgaaat ccatcccgaa aatcatggcc agcatctacg
     3061 agcgggatta tgtcaccgtg cccagggccg ccgaagaaca ggagctctat tcatcctctt
     3121 ttgatattca gaagaccatt cgggaattgc gaaaaaaaat gaagcaagcc gcggacacca
     3181 tggagttcga gaaagccgcc gaattccgcg accgcctcct ggccctcgag aagcgtcaga
     3241 tcgaggacgg cttatagccc gggaggccgt aaacccatgc cccgtgtgga catcgctctc
     3301 cggaatctga tagcatagg
//
LOCUS       DAOPBQ010000420         3315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23006_length_3315_cov_3.943558, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000420 DAOPBQ010000000
VERSION     DAOPBQ010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23006_length_3315_cov_3.943558"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..976)
                     /locus_tag="PKW60_12210"
     CDS             complement(23..976)
                     /locus_tag="PKW60_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HPK00241.1"
                     /translation="MDVAATRFQLARALPERSAPRRATAPGAFEAALSAASKAPSVRG
                     GKSIHTVAAGESLWSICRTHLGSTGNPPSAAAVSEAVQRLARANRIADADFILPGQQL
                     DLSVLRVRQAVNTPLARPVPGATAKIVPAGAGGPAIASNSVEEALWRAKKAALQTRGA
                     VAGLLKPESRAEADQGAEAPFNGLLGGSAFISSGFGLRKDPFTGRMQRHDGIDIAAAK
                     GTEVFALQEGRVFYSGWRGDYGRLVIVHHEDGTETRYGHLSDRLVKQGDTVKAGAVIG
                     KVGSSGRSTGPHLHFEVRQNARPINPLPYLQDRLLDIAQVF"
     gene            complement(1163..2449)
                     /locus_tag="PKW60_12215"
     CDS             complement(1163..2449)
                     /locus_tag="PKW60_12215"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPK00242.1"
                     /translation="MRNLSDPSGIPHIERDMAFLCGTLGARLAGSPEEERAADYMVDR
                     FRALGLTNPRKLPFPCMRWLPGAAAVHIEPGGPVLAAQQVAHSPATPPEGVEGELLFL
                     EPVDWENGLRCAELHGKIGLFLGGYGESERVFRELHQSRLAALLFVDTRLQTTWPIAN
                     GVGERFREHITKPMAYLSLMDAYKLARARVGRVRLVCSGIMADGSSWNAVAELPGGDP
                     DGRVIVVSGHLDSVAIGQGADDNATGIAATLECARRLQQDRHRHTLRFIGFGAEEQLS
                     VGAGRYVREQALDLDRIDWACNFDSIGAHFGLSEVMCTGTPEMEAYVRSVADTRLQYG
                     TVCADVCPYQDQFWFAAQGIPGVWFTRKTHLPGCWYHHSEHNTLEVVSCEEVAWAAEA
                     ACTLLSELADSEAWPFPRAIDPAMQAKVGGYLRELF"
     gene            2566..>3315
                     /locus_tag="PKW60_12220"
     CDS             2566..>3315
                     /locus_tag="PKW60_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HPK00243.1"
                     /translation="MFAYFKNRRRRRLRAEPFPEAWAGILEKNVPYYRHLPAEDRDEL
                     RQHVQVFLAEKHFEGCRGIQITDEIRVTVAGQACVLLLHRDTDYFPSLVTILVYPGAF
                     VVKTVIQSHEVFVVHGEEEREGEAWQRGVIVLAWDSVHEGFRHPHDGDNVILHEFAHQ
                     LDLQDSDADGLPADMPAALQPRWGQVLSKAYDALCRDVERGRPSVLDPYGATNPAEFF
                     AVVTECFFECPRELKQAYPELYDVFRAYYRQD"
BASE COUNT          622 a          987 c         1105 g          601 t
ORIGIN      
        1 caatgaggac gatctcgaag cactagaaaa cctgggctat atcgagtagc cggtcctgca
       61 ggtaggggag cggattgatc ggccgggcgt tctggcgcac ttcaaaatgg agatgcggcc
      121 ctgtggagcg gcccgacgaa cccaccttgc caattacggc gcctgctttc acggtgtcgc
      181 cttgcttgac caggcggtct gaaaggtgcc cgtagcgcgt ctcggtgccg tcctcatgat
      241 ggacgattac cagccgcccg taatcgcccc gccatccgct gtagaacacg cggccttcct
      301 gcagggcaaa gacttccgtg cctttcgccg cggcaatgtc gataccatcg tggcgctgca
      361 tgcgtcctgt gaagggatcc ttgcgcagcc cgaaacccga tgagatgaat gcggagccgc
      421 ccagcagccc gttgaaaggc gcctcggcgc cctggtccgc ctccgccctg gattccggct
      481 tgaggagtcc cgcaacggcg ccccgggtct gcagggcggc ctttttggcg cgccacaggg
      541 cctcttcgac ggaattggag gcgattgcgg gcccgccggc gccggccgga acgattttgg
      601 ccgtggcgcc cgggacaggc ctcgcgaggg gcgtgttgac ggcttgtctt acacgcaaga
      661 ctgacaggtc gagctgctgg ccgggcagga tgaagtcggc atcggcgatg cggttggcac
      721 gagccagccg ctgaacagcc tcggacacgg cggcggcact gggcggattg ccggtgctgc
      781 cgagatgcgt gcggcaaatc gaccacagag attcgcccgc ggccaccgta tggatggatt
      841 ttccgccgcg gacagaaggg gctttagacg cagccgaaag cgccgcctcg aatgccccgg
      901 gcgcggttgc ccggcgtggc gcgctccgct ccggtagcgc ccgggccagc tgaaacctgg
      961 tggcagcaac gtccatgatc cccctccaga gaatatcccg gcagttggga aagcaatact
     1021 catgcctttg cggccattcc ggcgtaaatt aattgtttac aagacttaca aaatggcgtg
     1081 ctcttgaaga ggggttgaag gccggaagat attgcccacg gccaggcgaa aagtgcagtc
     1141 ccgcacgccc agacggatgg cctcaaaaga gttcgcgcag gtatccgcca acctttgcct
     1201 gcatggccgg gtcgatggcg cgcgggaagg gccaggcctc agaatcggcg agttcggaga
     1261 gaagggtgca ggctgcctcg gcggcccagg cgacttcttc gcaggacacg acctcgagag
     1321 tgttgtgttc agaatggtgg taccagcagc cgggaaggtg agtcttccgg gtgaaccaga
     1381 cgccggggat gccctgtgcg gcaaaccaga actggtcctg gtacgggcac acatccgcgc
     1441 agacggtacc gtattggagg cgcgtgtcgg ccacactgcg gacataggct tccatctcgg
     1501 gcgtaccggt acacatgacc tcggacagtc cgaaatgggc gccgatactg tcgaagttgc
     1561 aggcccagtc gatgcgatcg agatcaaggg cctgctcgcg gacgtaacgg ccagcaccca
     1621 cggagagttg ttcttcagcg ccgaatccga tgaaacgcag cgtgtgcctg tgccggtcct
     1681 gttgcaggcg gcgcgcgcac tcgagagtgg ccgcgatgcc cgtcgcgtta tcgtcggcgc
     1741 cctggccgat cgcgacggaa tcgagatggc cgctcacgac aattacgcgg ccgtcagggt
     1801 ctccgccggg gagctcggcc acggcattcc aggagctgcc atctgccatg atccccgagc
     1861 agacaaggcg gacgcgcccc acacgcgccc gggcaagctt gtaagcgtcc atgagcgaaa
     1921 gataggccat gggcttcgtg atgtgctccc tgaaccgctc gccgactcca ttggcgatcg
     1981 gccaggtggt ctgcaggcgc gtatcgacaa aaagcagcgc cgcgagccgg gactggtgca
     2041 gttcccgaaa gacgcgttcg gattcgccat acccgcccag aaacaggccg atttttccat
     2101 ggagctctgc gcaacgcagc ccgttttccc agtccacggg ttcaaggaag agcagctcgc
     2161 cttcgacgcc ctcgggcggt gtggcggggg agtgtgccac ttgctgcgct gcgagcaccg
     2221 ggccgccggg ctcgatgtga accgccgcgg cgccgggcag ccagcgcata cagggaaaag
     2281 ggagtttacg gggatttgtg aggcccaggg cgcgaaaacg gtcgaccatg taatcggcgg
     2341 cgcgctcttc ttccggagag ccggccaggc gggcgccgag ggtgccgcac aggaaggcca
     2401 tatcgcgctc gatgtgtgga ataccgctgg gatcgctgag atttcgcatg gttgatgggc
     2461 gccctggtga acagtttgcg cggaccgggc ttggcacttt cgacgcgcgg cgattcggat
     2521 ggtagtatgc ggattcgaca atcgcgccgc aactgcgagg ggatcatgtt tgcttatttt
     2581 aaaaatcgcc gccgccggcg gcttcgtgca gaaccatttc cagaagcctg ggccggtatt
     2641 ctcgagaaga acgtcccgta ttaccgccat cttccggcag aggaccggga tgaactgcgg
     2701 cagcatgtcc aggtatttct ggcggagaaa catttcgaag gctgccgggg gattcagatc
     2761 accgatgaga tccgggtcac ggtggcgggc caggcctgtg ttctgctcct gcaccgcgat
     2821 accgactatt ttccatcgct ggtgaccatc ctggtgtatc cgggtgcgtt cgtggtaaaa
     2881 acggtgatcc agagccatga ggtattcgtg gtgcatggtg aagaagagcg ggagggagag
     2941 gcatggcagc gtggcgtgat cgtgctcgcc tgggattccg tgcacgaagg atttcgccat
     3001 ccgcatgacg gagacaacgt catcctgcat gaatttgcac accagctgga tctgcaggac
     3061 agtgacgcgg atggcttgcc ggccgacatg cccgccgcac tccagccgcg ctggggacag
     3121 gtgctcagta aagcctacga tgcgctgtgc cgggacgtcg agcgtggccg gcccagcgtg
     3181 ctggatccct acggcgcgac caatccggcc gagttttttg cggtcgttac cgaatgcttt
     3241 tttgagtgtc cccgcgaact gaaacaggcc tatccggaac tgtacgacgt gtttcgcgcg
     3301 tactaccggc aggat
//
LOCUS       DAOPBQ010000421         3311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23034_length_3311_cov_4.575553, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000421 DAOPBQ010000000
VERSION     DAOPBQ010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23034_length_3311_cov_4.575553"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKW60_12225"
     CDS             <1..342
                     /locus_tag="PKW60_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00244.1"
                     /translation="ARSYRAKARVQKRLRRVLDAGDPADALYRAIIGYIADKFDLPEA
                     GMTSSDVRDLFEGRAITQAASAHLLDILRTSERARYAGETLSRGAVEPLLQSAQESME
                     QLDLWLRKGSR"
     gene            318..1124
                     /locus_tag="PKW60_12230"
     CDS             318..1124
                     /locus_tag="PKW60_12230"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPK00245.1"
                     /translation="MVEKGIPLMWPLTVILCVLSAASSPEARFEEANAAYGAGRFDEA
                     AVLYEQVIADGIHDAVVFFNLGNACFSAGRLGPAVVSYERALRLEPGFERARVNLDHV
                     LASAQRRVAPPLPPWWEQTFFFWHGRLSPQWVTMAAALSWCAFWILLALRKYRRFAYA
                     RRLLALVAFVTLAFGISAWAKAHPAAIAVACQESVPVHYARGDLDTVHFELSAGDRVQ
                     VEERQSGWLLVSTGDGRRGWVDAAALALVGPPYDPVVPVMPGAPKTGGAG"
     gene            1486..2913
                     /locus_tag="PKW60_12235"
     CDS             1486..2913
                     /locus_tag="PKW60_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPK00246.1"
                     /translation="MKKHWFAAVIVLGVAVTVPLQSHAAMELLQQLENAFIEVGEKIR
                     PCVVNIDVKSAVPEGFSEDGQIPEDIFRFFNIPSPRERMPRPMPRREASGSGFIFSKD
                     GCIVTNNHVVEEAASIEVRFWNNRVLDATIVGRDPQTDLAVIKVEADFDLPTAVLGDS
                     DVLKVGQFSVAAGSPRGLEGSLSFGHITALGREGLNLGLRFQNLIQTDAAINLGNSGG
                     PLCNIDGEVIGINVAIVYGAESLGFAIPVNAAKKIIPILISDGRVTRGYLGVFIDAAT
                     DYAAALGLPDSKGAFVIDVSEDTPAARAGIKPDDVVRKVNGQVVENDQDLKVRISDIV
                     PGEIAKLEIWRDGAPIELDVKLEEFPESSQLVTQPPAEDILGLRTQPLTDQMRERLGL
                     DGDLAGVVVSEVKASSPADKAGIRVGDIVVRIGKYAVRNMDDFKRLMKEQAQPGGSVL
                     IRLFRGEEIPTVAVLKVPNDYVPEP"
BASE COUNT          606 a          993 c         1042 g          670 t
ORIGIN      
        1 gcacgctcgt atcgcgccaa agcgcgcgtg cagaaacgcc tgcggcgggt tctcgatgca
       61 ggagaccccg cggatgcgct ctatcgcgcc atcattggat atatcgccga caagtttgat
      121 ctccctgagg ccggaatgac ctcttcggac gtgcgtgacc tctttgaagg ccgcgccatc
      181 acgcaggcag cctctgccca tctcctcgac atcctgcgaa ccagtgaacg cgcccggtac
      241 gccggggaaa cgctttcccg cggcgcggtc gaaccgctgc tccagtccgc ccaggaaagc
      301 atggagcagc tggacctgtg gttgagaaag ggatcccgct gatgtggccg ttgaccgtta
      361 tactctgcgt tctgtcggca gcctcttctc cggaagcccg gtttgaagag gcgaatgcgg
      421 cgtacggcgc gggccggttt gacgaggctg ctgtgttgta tgagcaagtg atcgcggatg
      481 gcatccatga cgctgtggtg ttttttaatc tgggaaatgc ctgtttttcc gccgggaggc
      541 tgggcccggc cgtagtcagc tacgaacgag ccctgcgcct ggagcccggt ttcgaacgcg
      601 cgcgtgtgaa tctcgaccac gtgctggcgt ccgctcagcg ccgagtcgcc cctccgttgc
      661 ccccctggtg ggagcagacc ttctttttct ggcacggccg gctctctccc caatgggtga
      721 cgatggccgc ggccctgagc tggtgcgcct tctggatctt gctggctctc cggaaatacc
      781 ggcggtttgc ctatgcccga cggctgcttg cccttgtggc tttcgtgacg ctcgccttcg
      841 ggatctccgc ctgggccaaa gctcatcccg ctgcaattgc cgtggcctgc caggaaagcg
      901 ttcccgtgca ttatgcccgc ggcgatcttg acacggtgca ctttgaattg tccgccggtg
      961 accgcgtcca ggtggaggaa cgccagagcg gatggttgct ggtatccacg ggggacgggc
     1021 ggcgcggatg ggtcgacgct gctgccctgg ccctggtggg tcctccgtat gaccccgtgg
     1081 ttcccgtcat gcccggcgcg ccgaagacgg gcggtgccgg gtgacctgcc cccccgtggc
     1141 cagagtacgg gcaccttggc tgacgcgaga gatcgctccg cggccggttc tcgcgacgtc
     1201 tcggatgagc gccaatattt gctcttcccg cgccagcaat gtgatcactg tcgaaaccga
     1261 gggccgaagt gggttactct atggcatcat gtgtgccatg gcccagctga atttgaccag
     1321 ttcgattgcg cggctcgcaa cgagtgcccc gtggacgtgc ggctcgcgtc atgtggcgca
     1381 aaacaacggc aaaaccctga aaccggcacc gcaagcggaa atccgcaagg aaatccatac
     1441 agccatccat ccatgagctt cgatggacgc caaaggagag acagcatgaa aaagcactgg
     1501 ttcgcggccg ttattgttct gggggtcgcc gtgaccgtcc ctctgcaaag ccatgccgcg
     1561 atggaactgc ttcagcagct tgaaaacgcg ttcatcgagg tgggtgagaa gattcgtccg
     1621 tgtgtggtca acattgacgt gaaaagcgcg gtgcccgaag gcttttccga agacggccag
     1681 attcctgaag acatcttccg tttcttcaat atcccgtcgc cgcgtgagag gatgccgcgg
     1741 cccatgccgc gccgggaggc gtccggttcg ggattcatct tcagcaagga cggctgcatc
     1801 gttaccaaca atcacgtcgt cgaggaagcg gcttccatcg aagtgcgctt ctggaacaac
     1861 cgtgtgctcg atgccacgat cgtcggtcgc gacccgcaga ccgacctcgc ggtgatcaag
     1921 gttgaagccg atttcgacct gcccacagcc gtgctcggcg attctgatgt gttgaaggtc
     1981 gggcagttca gtgtcgcggc gggcagccct cgcggcctgg aaggctcgct gtctttcgga
     2041 cacatcaccg ccctgggccg tgaaggcctc aacctcggcc tgcgcttcca gaatctgatc
     2101 cagaccgatg ccgccatcaa cctgggcaac agcggaggcc ctttgtgcaa catcgatggc
     2161 gaggtgatcg ggattaacgt ggccatcgtc tatggagccg agtcactggg cttcgccatc
     2221 cctgtcaatg cagccaagaa gatcatcccg atcctcattt ccgacgggcg agttacgcgc
     2281 ggctatctcg gcgtgtttat cgacgctgcg acagactatg ccgcggcgct tggacttccc
     2341 gattccaagg gcgcgtttgt gatcgacgtc tctgaagaca cccctgccgc acgcgccggc
     2401 atcaagcccg acgacgtggt gcgcaaggtc aatggacagg tggtcgaaaa cgaccaggat
     2461 ctcaaagtga gaatctcgga tatcgtaccg ggtgagatcg cgaagctcga gatctggcgc
     2521 gacggcgccc cgatcgagct cgacgttaaa cttgaggaat ttcctgagag cagccaactg
     2581 gttacgcaac cacccgcgga agacatcctg ggccttcgta cccagcccct gacggatcag
     2641 atgcgcgaac gcctcggtct ggatggcgat ctcgccggcg tggtggtatc ggaggttaag
     2701 gcgagcagcc ccgcggataa ggccggcatt cgtgttggcg acattgttgt gcgcatcgga
     2761 aagtatgctg tccgaaatat ggacgacttc aagcggctca tgaaagaaca ggcccagcct
     2821 ggcgggtcag tccttatccg gctgtttcga ggtgaagaga ttcccacggt agccgtgttg
     2881 aaagtcccga acgactacgt gcccgagcct tagacaccgg tgtttggtgc gaacacgaac
     2941 tgccgggcgg gggagtctct gcctacagtt tggcctcgtg aactttgcga tgctaagctg
     3001 tggtcatgaa gccgatcctg tcgtgcatac tggttttcgc ggtgctcctg gtgtccggat
     3061 gcgattcgaa agcacctccc ataccatcac agtctgcccg ccagcgtgat gagacaggct
     3121 caacccggat tgaacgcaga accgcgtggg ggaaggggcg gaatttcccg gccggggaac
     3181 acaggcttcc attgggcgtg gcgccggaaa gaggcgcctt tcgagtgtct ctgcttgagg
     3241 attgggaaga gccggagccg gttcgcgtac agcttcgggc cggcgagacc gtgctccgca
     3301 cgttcgcgag t
//
LOCUS       DAOPBQ010000422         3310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23041_length_3310_cov_3.708141, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000422 DAOPBQ010000000
VERSION     DAOPBQ010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23041_length_3310_cov_3.708141"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /locus_tag="PKW60_12240"
     CDS             complement(<1..1716)
                     /locus_tag="PKW60_12240"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPK00247.1"
                     /translation="MSVFQTTILHVVSAVACLGLGALVLGRNPYKRAHRAFAALALNL
                     GLWTLGVVFIVHAHEPGWVLFWLRITFVIASFIPATYHSFLCVFPGHRFEGSRLLLVV
                     LLLLAVVSSIGAFTTYYVDPSQLRVSPDAPPGVTYGPVFLLLLASIGLTMLYGFPLLF
                     RKLWRSSGLERRQIQHVLLGTAAFTTLATVTNVVFPALGYENTEAYGPVFLVLMMGTL
                     AYAMVRYHLLDIWFILSRTTVYAILTAAVALIFVSTIFLVHWAFSSGSGRLSPGPTLL
                     AALVIAVVLEPLKEHAQLLLERTVLKRRYDVHALLRRVSRSASHTVQLDELLEQVGQD
                     IQETVGARSVRIMLVSETEPNVLITEYSTEPAEKEAVLRDYGELLHYMRHRNSPVALE
                     EMIHSRPTPEHARVAHHLAELEAYLCVPLRSMTDLIGLLTLGQKRTQDIYSSEDMAVF
                     ATLAGPLSTSIENARLYRKLEEANLHRARILASMRGGVLAVDTNGRVQTVNQAASEFL
                     GDVRPGYLLAALPEQIADVLERTLATGCDLEDYETRLRRPDGLETPVAMSASCLTSTD
                     GALKGA"
     gene            complement(1713..2957)
                     /locus_tag="PKW60_12245"
     CDS             complement(1713..2957)
                     /locus_tag="PKW60_12245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPK00248.1"
                     /translation="MRADIVITGIGVLACNGLGRQAFWDAIRNGRSGIRLIDRFDASE
                     MPCQIGGQLWEFNPDDFMKKSIVRTWCRHVHQAVASARLAAEDADLAGAGYSPDRIAT
                     GIGTSVGDPVEGYFEAMHAYESGGFKKVPRLSSSRFSGHSATVNVSIDFGFTGPAITI
                     SSGCATGLDGLEWGAEQIRRDRADAALVGATECPIFPLSFASGCSLGILSKRNDEPEK
                     AMRPFDRNRDGIVLSEAAVCVVLERAEHARARGARILAKILGFGSAAEAFSALALDPE
                     GKGLASAIQKALKNAGAVPADVDHVQAHGVSLEMYDRSETNSYKRALGEQAYRVPISA
                     VKSMAGQPYAVGGLLGLAAGALALETGRICPTLNLEDPDPECDLDFVPQRSRLNDVAT
                     VLVSAMSFGGTHSALLMGRPEQ"
     gene            complement(2965..>3310)
                     /locus_tag="PKW60_12250"
     CDS             complement(2965..>3310)
                     /locus_tag="PKW60_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00249.1"
                     /translation="LRARSRFGAPPGRWSVLPCRIGTLTSTQVLQCHESMIHEVELMG
                     VTAYMLHETPLVAHVLGWQHTLMEFRNEVMRRFLAGDVHGIARMVHDFNECASADVSV
                     AQGADLRAAVGN"
BASE COUNT          680 a         1063 c         1007 g          560 t
ORIGIN      
        1 ggcccccttc agggccccgt ccgttgaggt caggcaggac gccgacatgg ctaccggggt
       61 ctccagccca tccgggcgcc gcaggcgtgt ctcatagtct tccaggtcgc agcccgtggc
      121 cagagtgcgt tccagcacat ccgcgatctg ttccggcagc gcagcgagca ggtagccggg
      181 gcgaacatcc cccaggaatt ctgatgcggc ctgattcacg gtttgcaccc gcccgttggt
      241 gtccacagcg agcaccccac cgcgcatact ggccagaatc cgcgcccggt ggaggttggc
      301 ctcctcaagc ttccggtaca gccgcgcgtt ctcaatcgag gtggacaagg ggcccgccag
      361 cgttgcaaag accgccatat cctcgctgct gtaaatgtcc tgcgtgcgtt tctgccccag
      421 agtcagcagg ccgatgagat cggtcatgga tctcagaggt acgcacaggt aggcctccag
      481 ctcggccaaa tgatgcgcca cgcgggcgtg ctcgggagtg ggccggctgt ggatcatttc
      541 ttccagtgcc accggcgaat tgcgatgacg catgtagtga agcaactcgc cgtaatccct
      601 caaaacggcc tctttttcgg ccggttcggt ggaatactcg gtgatcagaa cgttgggttc
      661 ggtctcgctc acgagcatga ttcgcacgga gcgtgcgccg accgtctcct gaatgtcctg
      721 gcccacctgc tccagcagtt catccagttg caccgtgtgg ctggcggaac ggctgacccg
      781 gcgaagcagc gcgtgcacgt cgtaccggcg tttcagcaca gtacgttcca gaagcagctg
      841 cgcgtgttcc ttgagaggtt ccagcaccac ggcgatcacg agtgcggcga gcagggtggg
      901 gccaggggaa agccggccgg aaccgctgct gaacgcccag tgaaccagaa aaatcgtact
      961 gacgaagata agcgccaccg ccgccgtcaa gatggcgtac accgtggtgc gggagagaat
     1021 aaaccagata tcaagcaaat gataccgcac catggcgtag gcaagagtgc ccatcatcag
     1081 caccagaaag acggggccgt acgcttcggt gttctcgtag cccagggcgg ggaacaccac
     1141 gttggtcacg gtggccagag tcgtaaacgc ggctgtgccc agcagtacat gctggatctg
     1201 gcgccgttcg agccccgagc tgcgccacaa cttccggaaa agaagcggaa aaccgtagag
     1261 catcgtgagg ccgatggaag cgagcaggag caagaataca gggccgtagg tcacgccggg
     1321 cggggcgtcc ggcgacacgc gcaactgcga tgggtccacg taataggttg taaacgctcc
     1381 gatcgaagag accacggcga ggaggagcaa aaccaccagc aggaggcgtg aaccttcaaa
     1441 acgatgaccg ggaaacacgc acaggaaact gtggtaggtg gcgggaatga agctcgcaat
     1501 cacaaacgtg atccgcagcc agaagagcac ccagcccggc tcgtgtgcgt ggacgatgaa
     1561 gacaacccca agggtccata aaccgagatt cagtgccagg gcggcaaacg cgcgatgggc
     1621 ccgcttgtag gggttgcgcc caagcacgag cgcccccagt cccaggcagg caacggctga
     1681 gactacgtgc aggattgtgg tttgaaagac actcattgct ccgggcgccc catcaaaagg
     1741 gcgctgtggg tacctccgaa gctcattgca cttaccagca cggtggccac atcgttcaga
     1801 cgggaacgct gcggcacgaa atccaggtca cattccggat ccgggtcttc cagattcagc
     1861 gtggggcaaa tgcgcccggt ctcgagcgca agcgcccccg cggcgagccc cagcaggccg
     1921 cccacggcat acggctgccc cgccatcgac ttcacggcgg aaatcggtac gcggtacgcc
     1981 tgttcgccca aggcccgttt ataggaattg gtctcgctgc ggtcatacat ttccagagat
     2041 acgccatgcg cctggacatg gtctacgtcc gcgggaacgg ctccagcatt tttcaatgcc
     2101 ttctggatag cgctcgccag tcctttgcct tccggatcca gggccagcgc gctgaacgcc
     2161 tccgcggccg agccgaagcc caggattttc gccagaatcc gcgccccgcg ggcgcgcgca
     2221 tgctccgccc gctccagcac cacgcagacc gccgcctcgc tcaacacgat cccgtcgcgg
     2281 ttgcggtcaa acgggcgcat ggctttctcc ggttcatcgt tacgcttgga cagaatgccc
     2341 agggaacatc ccgacgcaaa cgacagcgga aaaatcgggc actcggtcgc accgaccagg
     2401 gccgcatcgg cgcgatcccg gcgaatttgt tccgcgcccc attcgagtcc gtcgagcccg
     2461 gtcgcgcagc cgcttgatat ggtgatggcg ggtccggtga atccgaaatc aatggacacg
     2521 ttaaccgtcg cggaatggcc ggaaaagcgg gaagacgaga ggcgggggac ctttttgaat
     2581 ccaccgcttt cgtacgcgtg catggcttcg aagtagcctt caaccgggtc gcctacgctg
     2641 gtgccgatgc ctgtggcaat ccggtcgggc gaataccccg ctcccgccag atcggcatct
     2701 tctgccgcca ggcgcgcgct cgcaaccgcc tggtgtacgt ggcggcacca cgtgcgcacg
     2761 atgctcttct tcatgaagtc atcggggttg aattcccaca gctggccgcc aatctggcaa
     2821 ggcatttcgg aggcgtcaaa gcggtcgatt agacggatgc cggaacgtcc gttgcggatg
     2881 gcatcccaga acgcctggcg ccccagcccg ttgcaggcca ggaccccgat cccggtaata
     2941 acgatgtccg cgcgcatacg ctcctcagtt gccgacggcg gcacgcaaat ctgcgccctg
     3001 cgccacactg acgtccgcgc tggcacactc attgaagtca tgcaccatgc gggcaatccc
     3061 gtgcacgtct cccgccagga agcgccgcat cacctcgttt ctgaattcca tcagcgtatg
     3121 ctgccacccc agtacgtgcg ccacaagagg cgtctcatgc agcatgtacg ctgtcacccc
     3181 catcaactct acctcatgaa tcatactttc gtggcattgc agcacctggg tcgaagtcaa
     3241 cgtgcctatc cggcagggca aaaccgacca ccggcccgga ggggcgccaa agcgtgagcg
     3301 cgcgcgcaac
//
LOCUS       DAOPBQ010000423         3308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23059_length_3308_cov_3.707654, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000423 DAOPBQ010000000
VERSION     DAOPBQ010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23059_length_3308_cov_3.707654"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..2360)
                     /locus_tag="PKW60_12255"
     CDS             complement(363..2360)
                     /locus_tag="PKW60_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide transport family protein"
                     /protein_id="HPK00250.1"
                     /translation="MDQRQLFLRDVLTVLFKRKAFILVFLILVFGVVLVGNYLWPPAY
                     ESIAKVRLIRGRTTSQPDPTVVHSEAGMLMMQLSREDVYSEIDLIYANDVLAKVVDQL
                     ELSSAMQRGTGPVQLAVRGFRELEYALRLKRRPDPRQQAIDALEESIEVKADDKKYVL
                     EIRCRLGDAQLAHDVLVALLEAYRQKHVDVFDQPGSAAFFEEQVARVTGELVEAQHKL
                     EAFRGEHQVVSLEAEKELLLSQYTEARKLLLQLTESQEAAQQAASGKSDQVLIATLSG
                     KTENTVVTELQLRLLELLLERNRITENLGPKHPQVVAILNEIDRAAERLSEAIEATRA
                     TTQAKITDLQRRIEELNKLEGELGDLQRTAETTADTLVYYTEKREEAIVADAMRDKQI
                     SSIRVVENPTVAVNPVSPRKLVNLFIALVVGIVGGVALAFFLEYLDHGLKTPEDVDYY
                     LKVPPLASFFHSGRRQALNKDEAQRLSTMIDAVQEGAPNQLIEVTSAVSGEQSGPVAA
                     ALAKARAENPEWRVLYIDFAPRRAEGAVPKGLTDLAAGRADLEETLNQDGNLYVLSRG
                     SEDCPGYLWESRGMQTALSDLRQRFDSIVLHTSPVRTSGDAINAARYADGVVIVVKAD
                     ATRREVVNRALDVLRHAKGRILGAVLTERKRTIPGMVYRRI"
     gene            complement(2414..>3308)
                     /locus_tag="PKW60_12260"
     CDS             complement(2414..>3308)
                     /locus_tag="PKW60_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPK00251.1"
                     /translation="YEKTGIIQGDPQISVNVDFANVDRLESISRDVTVRPNGAISLPM
                     LEREVKISGLTVAEASQAIADEAAKVLKNPPTVGLVVFPNINLALSGMNTVAKIRSDG
                     RISVPRVDYEIQAAGYGVDELRSMLKEAARGLIHNPVDVSVDVVQATGARIYVGGEVG
                     VPGVYPLDSCPTALQALIMARGPANSGRLNSVIVMRRNPDGKPYIFKTNLRAAMSKGY
                     TENDIPLKPFDVVFVPEKLIVRANIFVERYIDRIVPFDNSLGVNATYYMNEQQVNTKS
                     RNIGATAVVTPFQAGGLVVAP"
BASE COUNT          589 a         1091 c          949 g          679 t
ORIGIN      
        1 ctgcgataaa tggcgggcat gtaaacagtt gaatcatagg tggttgcgtt cttttgccat
       61 gccgtggtac aattcagttg gtcagcgcca catgtggcgt ttctccttcc gcggaggccc
      121 acggaacttg gttcctgggc ctctaaaata acttgcggcg ggttgagcac ggctccccgg
      181 cacgagttca gtcataagag gccggtgcgg acacgcaccg gcctctctta tcttgtcttg
      241 tgcaagaaaa tagctgccgc cccgtttccc tgcattgttc acaagatcaa ctcgcgaacg
      301 acgaagccgg cacgtacgga tgcgtggaac tctgcgacgg cgctgcgttc gtgcgtttcg
      361 cgctagatgc gccggtaaac catcccggga atggtccgtt tccgctcagt cagcacggcg
      421 ccgaggatgc gtcccttcgc gtggcggagc acatccagtg cgcggttcac cacttcccgg
      481 cgcgttgcgt cggctttaac cacgatcacc acgccgtccg cgtagcgggc agcattgatc
      541 gcgtcacccg aagtgcgcac gggggaggtg tgcagcacga tactgtcgaa acgctgccgc
      601 agatccgaca gtgcggtctg cattccacgg gattcccaga gatagccggg gcagtcttcg
      661 cttcctctgg acaaaacata gagattgcca tcctggttga gcgtttcctc gaggtcagcc
      721 cggcctgcgg ccaggtcggt caggcctttc ggcaccgcgc cttcagcgcg gcgcggcgca
      781 aaatcaatgt acagcacccg ccactcgggg ttttccgcgc gcgctttcgc cagggccgcg
      841 gccaccggcc ccgactgttc gcccgacacg gcgctggtca cctcgatcag ttgattcgga
      901 gcgccttcct gcacagcatc gatcatcgtg ctgagccgtt gagcttcgtc tttgttcaga
      961 gcttgtctgc gcccggaatg gaaaaacgac gcgagcggcg gcaccttcag ataataatcg
     1021 acgtcctcgg gcgtcttcag cccgtggtcc agatattcga ggaaaaacgc cagcgccacg
     1081 ccgcccacaa tgcccaccac cagcgcgatg aacaagttca ccagtttgcg cgggctgacc
     1141 gggttcaccg ccaccgtcgg gttttccacg acgcgaatac tcgagatctg tttatcccgc
     1201 atcgcatccg cgacaatggc ctcctcgcgc ttctcggtgt aatacaccag cgtgtcggcg
     1261 gtggtctcgg ccgtgcgctg caggtctccc agctcgcctt cgagcttgtt cagctcctcg
     1321 atgcgccgct gcaggtcggt gatcttggcc tgggtggtgg cccgtgtcgc ctcaatggcc
     1381 tccgacagcc gttccgcggc ccggtcgatt tcattgagaa tggccacgac ctgcgggtgt
     1441 ttcgggccca gattttcggt aatgcggttg cgctcgagca aaagctccag caaccggagc
     1501 tgcagctcgg tcaccaccgt attctcggtt ttccccgaca gcgtggcgat cagcacctgg
     1561 tccgacttgc ccgaggcggc ctgctgcgcc gcctcctggc tctcagtcag ctgcagcagc
     1621 agcttgcgcg cttcggtata ctggctgagc agaagctctt tctcggcttc cagcgacacc
     1681 acctggtgct cgccgcggaa cgcttccagc ttgtgctgcg cctccaccag ctccccggtc
     1741 acccgcgcca cctgctcctc aaagaacgca gcactgccgg gctgatcaaa cacatcgacg
     1801 tgtttctgcc ggtacgcttc aagcagcgcc accagcacat cgtgcgccag ttgcgcgtcg
     1861 cccagccggc atcggatctc caggacatat tttttgtcat cggctttgac ttcgatggac
     1921 tcttcgaggg catcgatcgc ctgctgccgg gggtccggac ggcgcttcaa ccgcagcgca
     1981 tactcgagct cgcgaaaacc gcgcaccgcc agctgcacgg ggcccgttcc gcgctgcatc
     2041 gcgctgctca gctccaattg gtccaccact ttcgccagca cgtcatttgc gtagatcaag
     2101 tcgatctcgg aatagacgtc ctcccgtgac agctgcatca tcagcattcc cgcctcgcta
     2161 tgcaccacgg tcggatcggg ctgggaggtc gtccgcccgc ggatcaggcg caccttcgct
     2221 attgattcat acgcgggcgg ccacaggtag ttgcccacca gcaccacgcc aaataccagg
     2281 ataaggaaga ccaggataaa cgccttgcgc ttgaacagca ccgtcaagac gtctctgagg
     2341 aaaagctgcc gctggtccat ctgacgctcc tcttgcagtt accttaccgc ggaatgcgcc
     2401 caggccgtcc gcgtcaggga gcgaccacaa ggccgccggc ctgaaacggc gtgacaaccg
     2461 ccgtcgcccc aatgttgcgt gatttcgtat tcacctgctg ttcgttcatg tagtacgtgg
     2521 cattgacccc gagggagttg tcgaacggca cgatacgatc gatgtaccgc tccacaaaga
     2581 tgttcgcgcg aacgatcagt ttctccggca cgaaaacgac gtcaaacggc ttcagcggaa
     2641 tgtcgttttc cgtgtagccc ttgctcatgg ccgcgcgcag attggtcttg aaaatgtagg
     2701 gtttcccgtc agggttgcgg cgcatcacaa tcacactgtt caaacgcccg ctgttggccg
     2761 ggccccgcgc catgatcagc gcctgcaatg ccgtcgggca gctgtcgagc gggtagactc
     2821 ccggcacacc aacctcgcca ccgacgtaaa tccgcgcgcc cgtcgcctga accacatcca
     2881 cggacacatc caccgggttg tggatcagcc cccgggcagc ctccttgagc atcgagcgga
     2941 gttcgtccac gccgtagccc gccgcctgga tttcataatc cacccggggc acggaaatcc
     3001 gcccgtccga gcgtatcttg gcgaccgtgt tcatgccgga gagggccaga ttgatgttcg
     3061 ggaagacgac caggcccacc gtgggcgggt ttttgagcac tttcgccgct tcgtccgcga
     3121 tggcctggga cgcctcggcc acggtcaatc ctgaaatctt cacttcccgt tcgagcatgg
     3181 gcaggctgat cgcgccgttg ggccggaccg tcacatcgcg gctgatgctc tcgaggcggt
     3241 cgacattggc gaaatcgacg ttcacgctga tctgcgggtc gccctggatg atgcctgtct
     3301 tctcatac
//
LOCUS       DAOPBQ010000424         3305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23093_length_3305_cov_3.301231, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000424 DAOPBQ010000000
VERSION     DAOPBQ010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23093_length_3305_cov_3.301231"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PKW60_12265"
     CDS             complement(<1..441)
                     /locus_tag="PKW60_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00252.1"
                     /translation="MTHLIFLSMTLALSAQADADKDSLHERAMARQSDLILGIYLTTD
                     AVERIFTLERDAAAMLRDYGFTLVTIEVYRSGQIVPIEHLARVRDYFRDHGFEVAGGI
                     ATTPGGDTGVRQKGPLAWYNWQNPKTQHDLEEIMRSVAPLFDTFV"
     gene            635..2014
                     /locus_tag="PKW60_12270"
     CDS             635..2014
                     /locus_tag="PKW60_12270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPK00253.1"
                     /translation="MRITSYGAVGGVTGSKHLLEVNGHKILFDCGMFQGVRREAAALN
                     RTLPFNPNDLDAVVLSHAHIDHSGILPMLARYGYKGRVFTTPATRDLCAIMLLDSAHI
                     QERDAQWLSKKHETFVPPLYTDVDVREIMRRFISIPYDINMEVAPGVSVTFKDAGHVL
                     GSAMLLVEFTENGSRRRFIYSGDIGQKNAAILKEPWEPDDADVVVMESTYGNRDHKPI
                     ETLDHDLAETINRTYEKGGKLIVPTFALERAQEFIFALKRLELGDHIPEMPVFVDSPL
                     TVNVTEVFRLHTDSFDDDIRSVMEESGDPFQLRHIEYIRDVKASMRLNGLKEPCIILS
                     ASGMCEFGRILHHLRNNIEDPRNTICIIGFQAKNTLGRRIVEREPTVKIFGLKHRLLA
                     DVKVFNSLSAHAGRSELIAFGKRFKDRAEKVLLVHGEPEALEALQSGLEAEGCTNIAI
                     QKLAEPAEF"
     gene            complement(2111..3034)
                     /locus_tag="PKW60_12275"
     CDS             complement(2111..3034)
                     /locus_tag="PKW60_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK00254.1"
                     /translation="MAIILIVEDDPQTAKYIQQNLADAGHTGIIEPTGEHATETAKAH
                     NCDLLILDIMLPGTSGFEVCRRFRRDPDLYMMPILILTAMKGDEELHHGLAQGADDYV
                     VKPFDINNLIHRMEALLRVSADRAGRDPIADIPAAGATKREVQRRVSLGEEFALAYIE
                     LQHIREFARQYGEEPRAKAIRRLARALQQAGRNFPSSAFFCGHMGGGHFVCVLPPGKA
                     NAYCDLVRRVWEHHLERFYTSIGHPQAYAAAKRRESSSPLLDVLICLTTREAKGMTAP
                     HELFETLSQLRAKASLARQGGIYVDRRGVNG"
BASE COUNT          725 a          937 c          945 g          698 t
ORIGIN      
        1 gacgaaggtg tcgaacaggg gggcaacgga acgcataatc tcttcgaggt cgtgctgggt
       61 tttcgggttt tgccagttgt accaagccag cgggcctttc tggcggacgc ccgtgtctcc
      121 accgggagtg gtggcgatgc cgcctgctac ctcgaaccca tgatcccgaa aatagtcccg
      181 cacacgggcc agatgctcaa tcgggacgat ttgaccgctg cggtacactt cgattgtcac
      241 cagggtaaac ccgtagtctc ggagcatcgc agccgcatcg cgctccagcg taaagatgcg
      301 ctcgaccgca tcggtcgtca gatatatccc caggatcaga tcgctttggc gggccattgc
      361 ccgctcgtgc agggagtctt tatctgcatc tgcctgggcg gagagcgcga gggtcatact
      421 gagaaagatc aggtgagtca tgggaatcct ccgttgatgt cgatggcccc gtgggccgta
      481 gagcacaccc taacgcaaat gggccgatga ggcaagagac aactttgaac agatggtttc
      541 tctggcggct gcgttgaatc cctctcccat ctgtgatatg cctacgtgaa ctggcagtgc
      601 attcacacgc cactgcaaaa aaggaattca tggcatgcgc attacgtcgt acggcgccgt
      661 cgggggagtc acgggaagca aacacctcct cgaggtcaac gggcacaaga tcctctttga
      721 ttgcggcatg tttcaaggcg ttcgccggga ggccgcggcg ctcaaccgca ccttgccgtt
      781 caaccccaac gatttggacg cggtcgtact gagccacgcc catatcgacc acagcggcat
      841 cctcccgatg ctcgcgcggt acggctacaa agggcgcgtg tttactacgc cggccactcg
      901 cgacctctgc gccatcatgc tgctcgacag cgcccacatc caggagcgag acgcacagtg
      961 gctgtccaaa aagcatgaga cgttcgtgcc gccgctgtat accgacgtcg acgtccgcga
     1021 gatcatgcgg cggttcatca gcattcccta tgatatcaac atggaggtgg cgccgggggt
     1081 cagcgtaacc ttcaaagatg ccgggcatgt gctgggctcg gccatgctgc tggtggagtt
     1141 cacggaaaac ggttcccgcc gccggttcat ctattccggg gatatcggcc agaaaaatgc
     1201 agccatactc aaggaaccct gggaacctga cgacgccgac gtcgttgtca tggaaagtac
     1261 gtacggtaac cgggatcaca agccgatcga gacgctcgac catgatctgg cggagacgat
     1321 caaccggacg tacgagaaag gcggcaagct gattgtcccc acgtttgcgc tggagcgcgc
     1381 gcaggaattc atctttgcgc tgaaacggct agaattgggg gatcatatcc ctgaaatgcc
     1441 agtgtttgtg gacagtccgc tgacggtcaa cgtgacggag gtatttcggc tccataccga
     1501 ctcatttgat gacgatatcc gaagtgtcat ggaagaatca ggcgatccct tccagctgcg
     1561 ccacattgag tacatccgtg acgtcaaggc atcgatgcgt ctcaacggcc tgaaagaacc
     1621 ctgcatcatc ctttctgcat cgggcatgtg cgagttcggg cgtatcctcc atcatctgcg
     1681 taacaacatc gaagatccac gcaacactat ttgcatcatt ggtttccaag cgaagaacac
     1741 gctcgggcgc cggattgtcg aacgagagcc gacggtcaag atattcggcc tgaaacaccg
     1801 tctgctggct gatgtcaagg tgttcaattc attgagcgca cacgcaggcc gctccgagct
     1861 catcgccttc ggaaaacgct ttaaagatcg ggcggagaaa gtgttgcttg ttcacggcga
     1921 gcccgaggcg ctcgaggccc ttcaaagcgg cctcgaggct gagggttgca ctaatatcgc
     1981 gatacaaaaa ctggccgagc cggctgaatt ctgaaccgca tgccctgtca ggcgctcgca
     2041 gaaaagaacg caggcagtgc aattgaggat ccccgggccg ggcattttcg ggcagaaagc
     2101 ggggctttct ttatccgttt accccccggc gatcgacata aatcccgccc tggcgcgcaa
     2161 gtgatgcttt tgcacggagc tgtgagaggg tttcaaagag ctcgtgcggg gctgtcatgc
     2221 ctttggcttc ccgggtggta agacagatga gcacatcgag caacggagag ctcgattctc
     2281 ttcgcttcgc cgccgcataa gcctgaggat ggccaatgct tgtgtagaat cgttccaggt
     2341 ggtgctccca gactcggcgc acaaggtcgc aataggcatt ggctttgccg ggtggcaaaa
     2401 cgcagacgaa atgcccgccg cccatatgac cgcagaaaaa ggcggaagaa ggaaaatttc
     2461 gtcccgcctg ctgaagggct cgggccagcc ggcggatcgc ctttgcccgg ggttcttcgc
     2521 catactggcg cgcgaattcg cggatatgct gaagttcaat ataggccagt gcgaactcct
     2581 caccaaggct gactctgcgt tgcacctcgc gtttcgtcgc accggctgcc ggaatatcgg
     2641 ctatcgggtc ccgtcccgct ctgtccgcgc tgacacggag cagggcctcc atgcgatgaa
     2701 ttaagttgtt gatatcaaag ggcttgacaa cgtaatcgtc ggcgccttga gccaaaccgt
     2761 gatgcagttc ttcgtcacct ttcatggccg tcagaatcag gatcggcatc atataaaggt
     2821 ccgggtcgcg ccggaaccgc cggcatactt cgaagccaga ggttcccggg agcatgatgt
     2881 cgaggatcag caaatcacaa ttgtgcgctt tggccgtctc ggttgcatgc tcacctgtag
     2941 gctcgatgat gccggtatgc ccggcatcgg caagattttg ctgaatatat ttggcagtct
     3001 gcggatcatc ttctactata agaataatag ccacatccct ctcctggcag gtctactgaa
     3061 agcttcgctg taaagccagg aatgcttcag ttggtgccct gcgccgcccg cttagataat
     3121 ctggacgaat cattctaaaa ctagcacagc tgcacgagag aacacaagac ctctggcacc
     3181 gggcatcccc gagccgtata gccgacttct tctgccctaa ttgcctttgg ggcctgcatt
     3241 cgtcgctcat gacaaagggt tctatgacgt taacatcaac ttcagcgtgc gtatttggta
     3301 aggcg
//
LOCUS       DAOPBQ010000425         3297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23151_length_3297_cov_2.835904, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000425 DAOPBQ010000000
VERSION     DAOPBQ010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23151_length_3297_cov_2.835904"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1639
                     /gene="malQ"
                     /locus_tag="PKW60_12280"
     CDS             101..1639
                     /gene="malQ"
                     /locus_tag="PKW60_12280"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009783942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPK00255.1"
                     /translation="MRLPRSSGVLLHPTSLPSPHGIGDLGPEARRFADFLHAAGQRIW
                     QVLPLGPTGHTHSPYQSYSAFAGNPLLVDLSALVQDGLLGSSELESAGVFSEASVDYD
                     AATAFKEEAMRRASHRFHGGAAPHLREEYDRFCAENAAWLDDYALFRAIKEAHGGGAW
                     HTWDASLARREPETLRAWQERLEPETLDLKFRQFLFFRQWHALREYCRERGIAIMGDI
                     PIYVAHDSSDVWSHPDLFHLDEEGKPTVVAGVPPDYFSATGQYWGNPIYRWDVLASQG
                     YAWWIDRIRSMFSMMDILRIDHFRGFESYWVIPATETTAVNGHWEAGPRDALFHAIQN
                     ALGAVPLVAENLGLITDEVEALRQRFEFPGMGVLQFAFGPDIDRAGFLPHQYYPNLVA
                     YTGTHDNDTLIGWWRAKGEGTTLSRAELQQERDFARRYMATSGKEIHWVGIRVIMASV
                     ADTAVFPLQDVLGLGSEARMNTPGTSGGQNWRWRYPAGALTSNVGDRLRELTEIYGRL
                     PGNR"
     gene            complement(1656..>3297)
                     /locus_tag="PKW60_12285"
     CDS             complement(1656..>3297)
                     /locus_tag="PKW60_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HPK00256.1"
                     /translation="NEAEAAQLVVRPTRPLNEFTAKASSLTGPHGAVLPAETIEILRV
                     RYVYVEFQTDAWGARDLWPDPLPPFTEPISLEAGKNQPLWVRVSIPRGAVPGVYSGTI
                     TLEADGFSAQVPLRVQVYAFELPDRMTCTTAFGFNAGTVYQYHALQDPEDREVVLNKY
                     LEYFARNHISLYDPVPGHGIQVEWVKLGEDEGAEFDPATRALLQEKDLTPRFAWSDWD
                     AAMESAIRDRHITTFQIGIPGLGGGTYMGHREPTLLDYKLGDPEFELAFNAYAKAVEA
                     HLRDKGWLDMSYVYFFDEPNPRQYEFVRDQYVRLKNAAPGIGRMITEEIAPALIGGPN
                     IWCPMTWTYNHDDAEERRAAGETIWWYICTHPKRPFAGVFIDHPGTDLRIWVWQTWQY
                     DVTGLLFWSTTLWTTSLAYPDHPQNPYEDPMGWNSSPYVKGAREPWGNGDGRFVYPPE
                     AAGGVASEPVLDGPVACYRMEMLRDGIEDYEYLAMLERLLDEKGDALPARTKRRFQSL
                     LTVPEEISKNLMTYTKDPAPIERHRDKVAKAIEALSAR"
BASE COUNT          628 a          997 c         1008 g          664 t
ORIGIN      
        1 ggtcgcagtc cggggtaaac cggggcccta tgtgaaggca ttccggaaaa cgcaggccgt
       61 gatacaggcc ctgttgcaga gctagagcaa aggaattgcc gtgcgcctac cacgttcaag
      121 cggagtgctg ttgcacccaa cgtccctgcc gtccccgcat gggatcggcg acctgggccc
      181 ggaagcgcgc cggttcgctg attttctgca cgcggccggg caacgcatct ggcaagtctt
      241 gcctttgggc ccgaccggcc acacccactc gccctatcaa tcctactcgg cctttgccgg
      301 caacccgttg ctggtcgatc tctccgcact tgtgcaggat ggcctgcttg gcagcagcga
      361 gctggaatcc gccggtgttt tcagcgaagc ctccgtcgac tacgacgcgg cgacggcatt
      421 caaagaagag gcaatgcgcc gcgccagtca ccgtttccac gggggcgccg cgccccattt
      481 gcgggaagaa tatgaccgtt tttgcgcgga aaacgccgcg tggcttgacg attacgccct
      541 gttccgtgcc attaaggaag cccacggcgg cggcgcctgg cacacctggg atgccagcct
      601 ggcacgtcgt gaaccggaaa cgctgcgagc ctggcaggaa cgactggaac cggaaacgct
      661 tgacctcaaa ttccggcagt ttctgttctt ccgccaatgg catgcgttgc gggagtattg
      721 ccgcgagcgc ggcatcgcga tcatgggcga tattccgatc tatgtcgctc acgacagcag
      781 cgacgtgtgg agccatcccg acctgttcca cctggacgag gagggcaaac ccacggtcgt
      841 ggccggtgtg ccgcccgact atttttcagc gaccggacaa tactggggga atcccattta
      901 ccggtgggac gttcttgctt cgcagggcta tgcgtggtgg atcgaccgga tccggtccat
      961 gttttccatg atggacatcc tgcggatcga tcacttccgc ggatttgagt cctactgggt
     1021 cattcccgcc acagagacca cggctgtcaa cggccattgg gaagccggcc cgcgggatgc
     1081 cctgtttcat gccattcaaa acgccctggg cgcggtcccg cttgtggcgg aaaatctggg
     1141 gctcatcacg gacgaagtcg aggcgttgcg ccaacggttt gaattccccg gcatgggcgt
     1201 gttgcagttt gcctttgggc cggacatcga ccgtgcgggc tttctgcccc atcaatacta
     1261 tccgaacctg gtcgcctata cggggaccca cgataacgac acactgatcg gctggtggcg
     1321 ggctaaaggc gaggggacga ccctgtcgcg ggctgaactc cagcaggagc gcgactttgc
     1381 acgccgatac atggccacct cggggaaaga aattcactgg gtaggaatcc gggtgatcat
     1441 ggcgtccgta gccgatacgg cggtgttccc cctccaggac gtcctcggcc tgggttctga
     1501 ggcccgcatg aacactcccg gcaccagtgg cgggcaaaac tggcgctggc ggtaccccgc
     1561 cggtgcgctg acgtccaacg tgggcgaccg ccttcgcgag ctgaccgaga tttacggccg
     1621 cctgcccggc aaccgttgac ataagcgccc gcacgctacc gcgcggacaa tgcctcgatg
     1681 gccttggcaa ctttgtcgcg atgacgttcg atcggcgccg ggtctttggt gtacgtcatg
     1741 aggtttttgg atatctcctc ggggactgtc agaagactct ggaagcggcg ctttgttcgc
     1801 gcggggagtg catcgccttt ctcatccagc aagcgttcaa gcatggcgag atactcgtag
     1861 tcttcgatgc cgtcgcgcaa catttccatg cggtaacagg ccaccgggcc atcgaggacg
     1921 ggctcactgg caacaccgcc cgccgcttcg ggcgggtata cgaaacgccc atcgccgttc
     1981 ccccacggtt cgcgtgctcc cttgacgtag ggggaggaat tccatcccat gggatcttcg
     2041 tatggattct gaggatggtc tggataggcc agggaggtcg tccagagggt ggtgctccag
     2101 aagagcagac cggtcacatc gtattgccag gtctgccaca cccagatacg caaatccgtg
     2161 cccggatggt cgatgaagac tcccgcgaaa ggccgtttcg gatgagtgca gatataccac
     2221 cagatggtct cacccgcggc ccggcgttct tcggcgtcat catgattata ggtccaggtc
     2281 atggggcacc agatattcgg gccgccgatc agtgccggag cgatctcttc ggtgatcata
     2341 cgcccaatac cgggcgcagc gttcttaaga cggacatact ggtcgcggac gaactcgtat
     2401 tgcctgggat tgggttcatc gaagaaatag acgtagctca tgtccagcca gcccttgtca
     2461 cgcagatggg cttcgactgc cttggcatac gcattaaacg ccagttcaaa ttcagggtcg
     2521 cccagcttgt agtcgagcag ggtgggttct ctgtgtccca tgtaggttcc gccgccgagg
     2581 cccggaatgc caatctgaaa cgtagtgata tggcgatcgc gaatggccga ttccatcgca
     2641 gcgtcccagt ctgaccaggc aaaccggggc gtcaggtcct tttcctgcag caatgcacgc
     2701 gtggccgggt cgaactcagc gccctcatct tcaccgagtt tcacccactc cacctggatc
     2761 ccgtggcccg gcaccgggtc gtacagggag atgtggttac gggcaaaata ctcgagatat
     2821 ttgttgagaa ctacttcgcg atcttcagga tcctgaagcg catggtattg atagaccgtg
     2881 cctgcgttga atccaaacgc agtcgtgcag gtcattcggt cgggcaattc aaaggcatag
     2941 acctgcacgc gcagaggaac ctgcgctgaa aaaccgtccg cttcaagcgt gatcgtcccg
     3001 ctgtacaccc cgggaaccgc gccgcgtgga atgctcactc gcacccacaa cggctggttt
     3061 ttacccgcct ccagcgagat tggttcagtg aagggtggca gcggatcggg ccacagatcg
     3121 cgcgcgcccc aggcgtccgt ttgaaactcg acatagacgt agcgcacccg caggatttca
     3181 atggtttcag cgggcagaac ggcgccgtgc ggcccggtca gactcgatgc tttggcggtg
     3241 aattcattca ggggccgcgt ggggcgtacg acgagttgcg cggcttcggc ctcattc
//
LOCUS       DAOPBQ010000426         3284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23266_length_3284_cov_2.912357, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000426 DAOPBQ010000000
VERSION     DAOPBQ010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23266_length_3284_cov_2.912357"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="PKW60_12290"
     CDS             <1..1094
                     /locus_tag="PKW60_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00257.1"
                     /translation="ISEPLLSAEPSGTPAPSPHQVALEEYKQRQQIRAQAEFEAAFEH
                     WSKLEDEQRSTLLTFATEQAAAAQAEWQQLAETAERTNQELNYRLETLRANIDRTMAE
                     REQAVNEVYNDALDNFDSDLYGYLNNREAKEADVLSYITGISPEEFAEDVALGYIRGN
                     RRRPNPYARANARRLADAADAAEAEAYWAEQVYNARIAADNRALRQVLDEQRNSERSL
                     RPALQRSAELVRRADALAQHLKTLDDALAASFEPKLSYRTLFSVDGTGVVQRDVNAAM
                     SLMRVDWWLDPTDSLAGQLTVRVYDNTTQKIVRTETSANKFPTNYFLIISEPGEYLIE
                     AEGPQGLTYTLEARELQDVELPPTGRGTP"
     gene            1260..2570
                     /locus_tag="PKW60_12295"
     CDS             1260..2570
                     /locus_tag="PKW60_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00258.1"
                     /translation="MSLSKKIRCILILSLVCGAAPNYADVITYRGREYRDVVIEELSN
                     QYVVHFPETGRRKYFQKEDVSSVEFSAETVRDPDEDEQDTPRQVRSGSDSKASNSSQP
                     PSEKITLEEYRRRSSMRAHVEFEAAYEHWCSLSDGQRRSFLFAAENQMQAADAQKKRT
                     EKKVAQGQEEMQKKLDHVAADIEANVLQRDSRIDAVYDEAYNDFDLRWNRGLRDAHAE
                     EAEVMQRFGRDGLADGHRFWEDVYTFKANRSRAQAFGQANAQARQIARESNARIAEAQ
                     QAYEQLARQAHKLERRNKIALQLEYGKARRTASVTQSLVALEQARLSAYEPWLNYREL
                     FSIEGNGVVQQAVLTVCPLLRIDWWVDGVISVPGQMKIRVYDAASGKLVTSVTSSNLP
                     VEHFLILTEPGDYTVEVEAPPELTYALEGKELLDDDSLFSIAEE"
     gene            2771..>3284
                     /locus_tag="PKW60_12300"
     CDS             2771..>3284
                     /locus_tag="PKW60_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPK00259.1"
                     /translation="MHSTNAPRWYVSLHGGHTAEFCDHGEGRLRDVLEAAVAKGFSSY
                     GVSEHAARVEDRFLYPEERDMGWTIEKVQGDFVRYAETVFAQAEEFAERLTVLRGMET
                     EVVPADRYAELMLGYRQRFSFDYMVGSVHYVDEMSIDSTTAQFEEAVEALGGLERLGI
                     RYYHTVAEMTA"
BASE COUNT          751 a          927 c          962 g          644 t
ORIGIN      
        1 ggatttccga accgttgctg tccgcggaac catccgggac acccgcccca tccccgcatc
       61 aggtggctct ggaagaatac aagcagcggc agcagatccg cgcccaggct gagtttgagg
      121 ccgccttcga gcattggagc aagctcgaag atgaacaacg gagcacgctg ctcacgtttg
      181 cgacggaaca ggccgcggcg gcccaggctg aatggcagca gctcgccgag accgccgaac
      241 gaacgaatca agagctgaac tatcggctgg aaaccctgcg cgccaatatc gaccgcacca
      301 tggctgagcg ggagcaggcg gtcaacgagg tctataacga tgccctggac aactttgact
      361 ccgatctgta tggatacctg aacaaccgcg aggccaaaga agcggatgtg ttgagttata
      421 tcaccgggat ttcgcctgag gagtttgcgg aagacgtcgc gctgggatac atccgtggaa
      481 accggcgccg gcccaatccg tacgcgcgcg ctaacgcccg gcgacttgcc gacgccgcgg
      541 atgccgccga agccgaggcc tactgggccg agcaggtcta caacgcccgc atcgccgccg
      601 ataaccgcgc gctgcgtcaa gtgctcgacg aacagcggaa ttctgagcgc agcctgcggc
      661 ctgccctgca gcgcagcgcc gagctggtac gccgtgcaga cgccctggcc cagcacctca
      721 aaacgctgga cgacgccctc gccgccagct tcgaaccgaa actcagctac cggaccctgt
      781 tttccgtcga cggtacgggc gttgttcaac gcgacgtgaa cgcggccatg tccctcatgc
      841 gggtcgactg gtggctggac cccaccgatt ccctcgccgg ccagctcacg gtgcgggtct
      901 acgacaacac cacgcagaaa atcgtccgga ccgagacttc cgccaacaaa ttccccacga
      961 attactttct aatcattagc gaacccggcg aatacctcat cgaagccgaa ggaccgcagg
     1021 ggctgacgta caccctcgaa gccagagaac ttcaggatgt tgagctgccc ccaaccggcc
     1081 gcggtacccc ctgacacgct cgcgcagagc gccctctccc gatcgccact ttttcattcc
     1141 caaactcagc gtatgacctc gcaagctcgt aatattgcac attaagacaa gaatgcacat
     1201 aatagatgtg gggtaaaaga cagaattcct cgtttttgag tcatggggga gaaatacgaa
     1261 tgagtttgtc gaagaagatc cgctgtatcc ttattcttag cctcgtctgt ggcgcggcgc
     1321 caaattatgc cgatgtgatt acctaccgtg gccgtgagta tcgcgatgtt gtcatcgagg
     1381 agctttctaa ccagtatgta gtgcatttcc ccgaaaccgg gcgacggaag tacttccaaa
     1441 aggaagacgt cagctccgtc gagttcagtg cggaaaccgt tcgcgatccc gatgaagatg
     1501 agcaggatac gcccaggcaa gtgcgttccg gcagtgattc aaaagcaagc aattcatctc
     1561 agccgccatc ggaaaaaata accctggaag agtacaggcg ccgctcgagc atgcgcgctc
     1621 acgttgaatt cgaagccgcg tacgaacact ggtgcagtct gtcggacggg caaaggagat
     1681 cctttctttt tgcggcggaa aaccagatgc aggctgctga cgcacagaag aaacgtactg
     1741 aaaaaaaggt ggctcagggc caggaggaaa tgcagaaaaa gctggaccat gttgccgcgg
     1801 atattgaagc taatgtcctc caaagagact caaggattga cgcagtttat gacgaagcct
     1861 acaacgattt tgatctccgg tggaaccgcg gtctgcggga tgcgcacgct gaagaagccg
     1921 aggtaatgca gagatttgga agagacggtc ttgcggacgg ccatcgcttc tgggaggacg
     1981 tgtatacgtt caaggctaac cgttcacgcg cgcaggcctt tggccaggca aatgcccagg
     2041 ctaggcagat tgcgcgtgag tctaatgccc gtatcgcgga agcacagcag gcgtacgagc
     2101 agctggccag acaggcacat aaactcgagc gccgtaacaa aatcgccctc cagcttgagt
     2161 atgggaaagc tcgacgcacg gcttcagtga cgcagtccct tgtcgcgctg gagcaggctc
     2221 ggctcagtgc gtatgaacca tggctcaact acagggagtt gttttcgatc gaggggaatg
     2281 gggttgtcca gcaggctgtt ctgactgtct gtcccttatt gagaatcgat tggtgggttg
     2341 atggcgtgat ttcagtgcct gggcagatga agattcgcgt gtatgatgcg gcatctggga
     2401 aactggtaac atcagtgacc tcatccaatc tgccggtaga gcatttcctt attctcacag
     2461 aaccagggga ttatactgtt gaggtcgagg cccctcccga actgacctac gcgctcgaag
     2521 gcaaagaact tcttgatgat gactctctct tttcgatcgc agaagaataa acgaagagcg
     2581 actttttcgc ccgggaacac gtgtttcgcc ggatatcgtg tgtttcctcg tctgtgagac
     2641 agcaggtatt ggcagactca aaccgatttt cccgccccag gcgcccttgc ttcacatgtc
     2701 ttttgaagca gggcaggccg atttggtacc gtgcgcgcgc cggcgtgctg ccgggttcac
     2761 aggaggtctc atgcattcca cgaatgctcc ccgctggtac gtctcgctgc acggcgggca
     2821 tacggctgaa ttctgcgatc acggcgaagg ccgcttgcgt gacgtgctcg aagcggctgt
     2881 cgcgaaaggt ttttcaagct acggcgtaag cgaacatgcc gcgcgcgtcg aggatcggtt
     2941 cctctatccc gaagaacgcg atatggggtg gacaatcgaa aaggtgcagg gcgattttgt
     3001 ccggtatgcc gaaaccgtgt ttgcgcaggc agaggaattc gccgagcgcc tgaccgtttt
     3061 gcgcgggatg gagaccgagg tggtgccggc ggatcgttac gcagagctga tgctcggata
     3121 ccgccagcgc ttttccttcg actacatggt gggctcggtg cattacgtgg atgaaatgtc
     3181 catcgacagc accactgccc aattcgaaga ggccgttgaa gcgctcggag ggctcgagag
     3241 gctcggcatc cgctactacc acaccgtcgc ggaaatgacg gctg
//
LOCUS       DAOPBQ010000427         3275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23349_length_3275_cov_4.005901, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000427 DAOPBQ010000000
VERSION     DAOPBQ010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23349_length_3275_cov_4.005901"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PKW60_12305"
     CDS             <1..337
                     /locus_tag="PKW60_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00260.1"
                     /translation="YADLLNRTGRYAEAADFIAALPEHIRSHERLLLAQARAALQTGR
                     FEHVEQIFETEFAQIRECETSLTDLWFAYHEQRIAEAEGIPIDDALRERVRRDYVPPR
                     NIDFRMSPG"
     gene            699..1529
                     /locus_tag="PKW60_12310"
     CDS             699..1529
                     /locus_tag="PKW60_12310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPK00261.1"
                     /translation="MQRAKDGSLSTYLGEISRIPLLSTTEEVRLARRALQGDGEARRK
                     LIVSNLRLVVSIAKKYLYYGLPLQDLIEEGNVGLLKAVERYDPERGCKFSTYATWWIR
                     QAITRALSNYGRTVRIPVYVTDNIAKYKKAAEELYIRTGKKPEPEDVAQVLGIKVTEA
                     RKLQSFVEDVSPLDKLDTTDDDSGRGIPESLEPRRIDDAIAQIEMDQQLEQLMQQLTE
                     READIMRFRYGLMDGEAHTLEETGKQFGLTRERIRQIEKDVMRRLRVFVAERADDFRL
                     "
     gene            1654..2175
                     /locus_tag="PKW60_12315"
     CDS             1654..2175
                     /locus_tag="PKW60_12315"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPK00262.1"
                     /translation="MDLAQFIRNVPDFPKAGIQFKDITTLLKNGPAFKSVIDGWRERY
                     AGQGIDAVVGVEARGFMFGGALAYALQTPFVPVRKPGKLPSETLSETYHLEYGTDAVE
                     IHKDALKPGDRVVMIDDLLATGGTMGAAVRLIERLGAEVFEIAFLIELPSLKGRGQLQ
                     GYPVHALLQFMPE"
     gene            2185..3024
                     /locus_tag="PKW60_12320"
     CDS             2185..3024
                     /locus_tag="PKW60_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPK00263.1"
                     /translation="MKGTTLPGTEINIINDVERGRFKHVLFDFDGTVSLLREGWQLIM
                     GPLMVEMISGGGSPSCLPEIEDDVREYIEESTGIQTILQMERLVEMVQARGLVPQDQI
                     LDAQGYKKIYNDRLMVRVNERIRELENGTLTPLDVTLRGALDFLERLSRKNVTMYIFS
                     GTDRDDVRNEARLVGVAPYFQEIWGALREYKDYNKEMVLKEIIATNDLHGSEVLIVGD
                     GPVEIRNARDNDCVSIGVASDEIQGFGWNMEKYPRLVNAGADIMVPDFSDASALIDYL
                     FPG"
     gene            3068..>3275
                     /locus_tag="PKW60_12325"
     CDS             3068..>3275
                     /locus_tag="PKW60_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK00264.1"
                     /translation="MEEHELSCDILVAGGGPAGVSCALAAARLGASVILCQDRPVLGG
                     NASSEVRMHNVGADCSGGRGSELET"
BASE COUNT          697 a          896 c         1053 g          629 t
ORIGIN      
        1 atacgccgat ctgttgaatc gaaccgggcg ctacgccgag gctgcggact tcatcgcggc
       61 ccttcccgag cacatccgca gccacgaacg cctgctcctg gcccaggccc gggccgcgct
      121 ccaaacaggg cgatttgagc acgttgaaca aatttttgag actgaatttg cacaaattcg
      181 cgaatgcgag acttcgctca ccgacctctg gttcgcgtac cacgagcagc gaatcgcgga
      241 agcggagggt attccgatag atgacgcctt gcgggaacgg gttcgccgtg actatgtgcc
      301 tccccggaac attgattttc gcatgtcccc gggatgagcc ctttctgccg ccggcgggct
      361 cgcagttgag cccggggggt catgatttgc gtctcgaata tcgtgacgcc gcccgctgtt
      421 tgtctagtga agggcttgtc gtttgtgcgc tgcgatccgc ctgtcggcag cgattttcag
      481 acgagctgcc tgcgaaaacg tcgaaaaagc gcgaaagtcg cggcttgaat gtcgtgaaac
      541 acagtgcaac caggccaaat cgtttgacaa gtaacggtaa accggactac cgtaaggcgt
      601 tgcagatgga aaacgcctta cacgtctcat cgggtttgcg cgagtccgga agcatttttt
      661 gagtcaacgt gggtgtcgcg ctcaactgag gcaacggcat gcagcgggct aaggacggca
      721 gcttaagtac ctatctaggc gagatctcca ggatccctct gctttcaacg accgaagaag
      781 tgcgtctggc gcggcgggcg ctgcaggggg atggcgaggc gcgccgcaag ctgatcgtct
      841 ccaaccttcg cctcgtggtc agcattgcaa agaaatatct ctattacggc ttgccgctcc
      901 aggacctcat tgaggaagga aatgtcgggc tgctgaaagc ggtagagcgg tatgatcccg
      961 agcgaggctg caagttttcg acctacgcca cctggtggat ccggcaggcg atcacgcgtg
     1021 cgctgtcgaa ctatgggcgc acggtgcgca tcccggtcta cgtgaccgac aacatcgcaa
     1081 aatacaaaaa agccgcggaa gaactctaca tccgcaccgg caagaagccc gagccggaag
     1141 acgtcgcgca ggtgctgggc atcaaagtca cggaagcgcg gaagctgcaa tccttcgtgg
     1201 aagatgtcag cccgctcgat aaactggaca ccacggatga cgactcgggc cgcggcatcc
     1261 ccgagagcct cgaaccgcgc cgcatcgatg acgcgattgc gcagatcgaa atggatcagc
     1321 agctggagca gctcatgcag cagttgaccg agcgcgaagc ggatatcatg cgtttccggt
     1381 acggattgat ggatggcgag gcacacaccc tcgaggaaac gggcaaacag ttcggattaa
     1441 cccgggaacg catccggcaa atcgagaagg acgtgatgcg tcggctgcgc gtgttcgttg
     1501 cggaacgcgc ggatgatttc cggttgtaag gggaaagcga aagaaccgcc cgccgcgcac
     1561 cacggttgaa tcgcaccccg tttcgcctgt ataacgctgc gcgccgacgg agtgtaaagc
     1621 tctgttgctt gaaaaaagga ggggtgctac gtcatggatt tggcgcagtt catccgcaac
     1681 gtgccggatt ttccgaaagc gggcatccag ttcaaagaca tcacgacgct tctgaaaaac
     1741 ggcccggcct tcaagagtgt catagatggc tggcgggagc ggtacgcggg ccagggcata
     1801 gacgcggttg tcggtgttga ggcccgcggg ttcatgtttg gcggcgcgct cgcatacgcg
     1861 cttcagacgc cctttgtccc ggtgcgcaaa ccgggcaagc tgccgtccga aaccctctcg
     1921 gagacgtacc atctggaata tgggacggac gccgtggaga ttcataagga tgcattgaaa
     1981 ccgggggacc gggtcgtcat gatcgacgat ttgctggcta cgggcggcac gatgggcgcc
     2041 gcggtgcggc tgattgaacg gcttggtgcc gaggtatttg agattgcgtt tttgattgag
     2101 ttgccgtcac tgaagggtcg cgggcagctg cagggttacc cggtgcatgc gctgctccag
     2161 tttatgccgg aataacgagg tactatgaaa ggcacaacac ttcctggaac tgaaatcaac
     2221 atcatcaatg acgtcgaacg gggccgtttc aaacacgtcc tgttcgattt tgacgggacg
     2281 gtcagcctgt tgcgcgaagg ctggcaactc atcatgggcc cgctcatggt cgaaatgatc
     2341 agtgggggcg gctctccgtc gtgcctgccg gagatcgaag acgatgtgcg cgaatacatc
     2401 gaagaatcca ccggcattca gactattctg cagatggagc ggctggtcga aatggtgcag
     2461 gctcgcgggc ttgtgcccca ggaccagatc ctcgatgcac agggatacaa gaaaatttac
     2521 aacgaccgcc tcatggtgcg cgtgaatgag cgaatcagag aactggaaaa cggcacactc
     2581 acgccgctgg acgttacgct gcgcggcgcg ctcgatttcc tcgagcgcct ctccagaaag
     2641 aatgtgacca tgtacatctt cagcggaacg gaccgcgatg atgtgcgtaa cgaagcccgc
     2701 ctggtgggtg tcgcgcccta cttccaggag atctggggtg ctctccgcga gtacaaggat
     2761 tacaacaagg agatggtgct caaggagatc atcgcgacga acgatctgca cggttccgaa
     2821 gtgctgattg tgggcgacgg ccccgtcgag attcgcaatg cccgggacaa cgactgcgtg
     2881 tcgatcggtg tcgcatcgga cgaaatccag gggtttggct ggaacatgga gaagtatccg
     2941 cggctggtaa acgcaggcgc ggatatcatg gtgcccgatt tctcggatgc ctccgcactg
     3001 atagactacc tgttcccggg ctgagccggc ggaggcagac gggccggggc gtggaagaga
     3061 ggtccttatg gaggagcatg agctgagctg tgacatcctg gtggcgggcg gtggtcccgc
     3121 gggcgtttcg tgcgcgctgg ctgcggcgcg cctcggcgcc agcgtgatcc tgtgccagga
     3181 tcgcccggtc ctgggcggca acgcctcctc tgaggtccgc atgcacaacg tcggggcaga
     3241 ctgttcgggc gggcgcgggt cggagctgga gacgg
//
LOCUS       DAOPBQ010000428         3270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23395_length_3270_cov_4.021462, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000428 DAOPBQ010000000
VERSION     DAOPBQ010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23395_length_3270_cov_4.021462"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2038)
                     /locus_tag="PKW60_12330"
     CDS             complement(<1..2038)
                     /locus_tag="PKW60_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPK00265.1"
                     /translation="MSNRTCRCLGTKQTGFQVRLAAILCVAIGLAAGSAAFAAPLEPL
                     RAVYLHPAPFADGAVTLEARAARIAEILSDARDCGLTTLLPYANTSGGRAYYPSRYLR
                     PAGKAGDDTLGLLAREAQARGLSLMPAICVLVSGYDLPQGILETHPDWALRKPSGEPM
                     GWISPAHAEARAWVVGMIQEIVAHVQPAGVLLDYLRYPNEEVLLDPASAAAFDAASPP
                     DEAEAARKERLQRFKEESLNILAEEINRALRKQQPELRIGLYTWGPHVPFNHPVAQCW
                     PDWIHNNYIDIVNVSGYCYRKNYGEEYLDVFEKRIRDSVELAQQAGAPVSLSFALGMH
                     TSHGAIESASEVATYRSLAEKAGCPGVAVFAWAGLEKFLPDVIQGEYLRDVTPGATAA
                     AGWKARLTVDLGVDQGQNFGSLFEAVGPDGKPVAGAGFLGAYNTYYRASRHMLHFYVK
                     PPHAQDSVQATRFPRPSDSCHHYLFDIADTVYAVDRTGTPPIHRWETEAGQWTATDPP
                     QAVAFDVGSHRLECFSNRLTVDGREAFRFDASQGTAGSFYYAQGWLFFHVYEADSPER
                     RTAIHACPWDLEAQPEPDLSQTVVLPLTAPGEFLYAYGQLNNDVYVGSNNGGFYRFRQ
                     GQWSTLRKADPTTSFQLYAMVNYRERLLMGQYPSGELFEVEGDELKQLNGWPPCP"
     gene            2404..3264
                     /locus_tag="PKW60_12335"
     CDS             2404..3264
                     /locus_tag="PKW60_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00266.1"
                     /translation="MNETRATRCIRNRTALFRYFGIAMGVLCGIACSTVPKPEARLPA
                     ETPVAASEPAEEDAASTEPLRWHFLAATVNTYPRLESERLVDKFFNRPMRLLAPGFDD
                     VRTFNDVSDDGLMWPPHVGIGRVLGDRWDVFFEAGYSAGKVRTKATDPSLLVLPLHTD
                     FEILRQALYAGVGVDYYPWRTVTLGEYHGLAERLHAARPFVGSRLTWTWARYNARAKV
                     GFEPFGTLVRVQLADEWVIPSVNLCIGADVPLTPKSLVTVNAGYNFFKERGYDFEGPA
                     FTLGWKYFFR"
BASE COUNT          615 a         1022 c         1003 g          630 t
ORIGIN      
        1 gcgggcacgg cggccagccg ttcagctgtt tcagctcatc cccctccact tcaaacaatt
       61 ctccggaggg gtactggccc atcaacaggc gctcccggta attgaccatg gcgtagagct
      121 ggaagctggt cgttggatcg gcctttcgaa gcgtagacca ctgcccctga cggaagcggt
      181 agaacccgcc gttgttggag cccacataca cgtcgttatt cagctgtcca taggcataca
      241 aaaattcgcc cggagcggta agcgggagca ccaccgtttg gctcaagtcc ggctccggct
      301 gagcctccag atcccacggg catgcgtgga tggccgtgcg gcgctccggc gaatccgctt
      361 cgtacacgtg aaagaacagc catccctgcg catagtaaaa ggagccggct gtaccctggc
      421 tcgcgtcgaa acgaaacgcc tcccgcccgt ccacggtgag ccggttcgaa aaacattcca
      481 atcggtggct gcctacatcg aatgccacgg cttgcggcgg atcagtggcc gtccactggc
      541 ccgcttccgt ctcccagcgg tggatgggcg gcgtgcccgt gcggtccacg gcgtatacgg
      601 tgtcggcgat gtcgaacagg taatggtggc aactgtccga cggccgcggg aaccgcgtgg
      661 cctgtaccga atcctgggcg tggggcggtt ttacatagaa atgcagcata tggcggctgg
      721 cgcggtaata cgtgttgtat gcgcccagga accctgcccc ggccaccggt tttccgtccg
      781 ggcccacggc ttcaaagaga gagccgaagt tctgcccctg gtcgactccc aaatcaacgg
      841 tcaacctggc cttccatccc gccgctgcgg tggcgccggg ggtgacgtca cgaaggtact
      901 ctccctgaat cacatcgggc aggaattttt ccaaccccgc ccaggcgaag accgccacac
      961 cggggcagcc tgctttttcc gcgagcgagc ggtacgtcgc aacctccgac gccgattcga
     1021 ttgcgccgtg actcgtgtgc attcccagcg cgaaggagag ggaaacgggc gccccggcct
     1081 gctgcgccag ttccacggaa tcgcgaatgc gtttttcgaa gacatcgagg tattcctctc
     1141 cgtaattctt gcgatagcag tagccggaga cattcacgat gtcgatatag ttgttgtgta
     1201 tccagtccgg ccagcattga gctacggggt gattgaatgg gacgtgcgga ccccaggtat
     1261 agaggccgat ccggagctca ggctgctgtt tccggagcgc ccgattgatc tcctccgcca
     1321 ggatgttgag cgactcctct ttgaatcgct gcagccgttc tttacgggcc gcctccgcct
     1381 catccggagg gctggccgca tcaaaggccg ccgcgcttgc cggatccagc agcacctcct
     1441 cattggggta gcggagatag tcgagcaaca ctccggctgg ttggacatgc gccactattt
     1501 cctgaatcat gccaaccacc caggcgcgcg cctcggcatg cgcgggagaa atccagccca
     1561 tgggttcgcc ggaaggcttt cggagcgccc agtcggggtg cgtctccaaa atgccctggg
     1621 gcaggtcgta gcccgaaacc agcacgcaaa tcgccggcat caggctcaac ccgcgcgcct
     1681 gggcctcccg tgcgagcagc ccgagcgtgt catcgcccgc tttccctgcc gggcgcaggt
     1741 agcggctcgg ataataggcg cgtccgccgg acgtgttcgc gtaaggcagc agcgtggtca
     1801 atccgcagtc gcgggcatca ctcagtatct ccgcaatccg agcagcgcgc gcttccaggg
     1861 tcactgcgcc gtcggcgaac ggcgccgggt gcaggtagac cgcccgcaac ggttccaacg
     1921 gggccgcgaa ggccgctgaa ccggccgcca acccaatcgc aacacacaaa atggccgcga
     1981 gccgcacctg gaaaccagtc tgttttgtgc cgaggcaccg gcatgttctg ttactcatgc
     2041 gtcctccatt ttccccgggg aagagcgaaa agtgggtagc cgccagccgg cagccatctc
     2101 tgcgccttcg cctcaggtta tttgtctgga atgcggagca ttatatatga acggccgcca
     2161 cggtagcagg agttgccggc catcacgccg gggaccggca cggagttccc gggcgggggt
     2221 tgataccacc tcaacaagaa ggatagtatt ccccccggca cgtgaacaag acatggtagg
     2281 cggcataacc cgggttccac cctgtttagc ggactgatac ccggatcgtt gggactgtgg
     2341 agcgagttcc acggttccgg ggcggttcca atccgcataa ttctatctcc tgggaaatag
     2401 tacttgaacg aaacccgcgc aactcgatgc attcgcaacc gaaccgcgct gttccgctat
     2461 tttggaattg cgatgggcgt actgtgcggt atcgcgtgct caaccgtgcc caaacctgag
     2521 gcgcggctcc ccgccgagac gccggttgct gcttcagagc cggccgagga agacgccgcc
     2581 agcacagagc cgctccggtg gcatttcctt gccgcgacag tcaacacgta tcccaggctg
     2641 gaaagcgaac ggctcgttga taaattcttt aaccggccga tgcggctgct ggcccctggt
     2701 tttgacgatg ttcgcacttt caatgatgtg agcgacgacg gcctgatgtg gccccctcat
     2761 gtcggtattg gacgggtctt gggcgaccgg tgggatgtgt ttttcgaagc gggctattcc
     2821 gcgggcaagg tgcggacgaa agccacggat cccagcctgc tggtcctgcc gctgcatacg
     2881 gatttcgaga tcctgcggca ggcgctctac gcgggcgtcg gcgtggatta ctacccgtgg
     2941 cggacggtca cgctcgggga ataccacggg ctcgcggaac gcttgcacgc ggcgcgcccc
     3001 tttgtcggca gccggctcac ctggacctgg gcccggtata atgcccgggc caaggttggc
     3061 tttgaaccgt tcggcaccct ggttcgagta cagctcgccg acgaatgggt gatccccagc
     3121 gtgaacctgt gtatcggcgc cgacgtgccg ctgactccca aaagcctggt taccgtgaat
     3181 gccggataca attttttcaa agaacgggga tacgactttg aaggcccggc cttcactctg
     3241 ggctggaagt attttttcag atgacaacag
//
LOCUS       DAOPBQ010000429         3270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23403_length_3270_cov_3.485848, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000429 DAOPBQ010000000
VERSION     DAOPBQ010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23403_length_3270_cov_3.485848"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="PKW60_12340"
     CDS             complement(<1..1513)
                     /locus_tag="PKW60_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HPK00267.1"
                     /translation="MRNWWRRVLLQPMRAAVLMTFLWALLLGGCGGPAAQPGSAPDTE
                     DAGAASTASAEMDGEPEYGDWLITRMSAETENLNPYTSSDAYASRINAFIFESLLERD
                     NETLEMIPRLAESWEISDDKLAYTFTLHEGTVFSDGTPLTTEDIKFSFDTIKDPKVDA
                     PHLRNYYQDVTACEVIDARTVRFVCSKPYFRHIVMLGGLDIIPRHIYGVGDFNAHPNN
                     RKPIGSGPYMLEKWDPGQQMVLVENPVYWGKRPYLAKRVYRIILSDGPALQALLGGQL
                     DTMGLTPEQWVRQTGSKRFTGQFNKFSFYAPYYNYIGWNSRRPQFSDKRVRRALTMLL
                     NREDIREKIFHGLAITVTGNAFVESPEYNKNIQPWPFDPEQAKQLLTEAGWVDSDGDG
                     LRDKDGTVFRFDLLIKNDSPETEQIATIFQNELKRAGIEMSLRPLEWATFLQQIDARK
                     FDACILGWSMPADFDPYQVWHSSMAEGTGSNFVGFNNAEADQIMETARQTFDRD"
     gene            complement(1679..2740)
                     /locus_tag="PKW60_12345"
     CDS             complement(1679..2740)
                     /locus_tag="PKW60_12345"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HPK00268.1"
                     /translation="MPKLQIGSITAEVPIIQGGMGVKVSLASLAAAVANEGGIGTIAS
                     VGLGDMHTTREDYEGVSSEAIAEEIRKARSATHGPIAVNVMSVLSNAETLIQTSVREG
                     VKMVVVGAGLPLKLPAMVEDDSVNLVPIVSSGRAAALILRTWEKRYGRTAQAVILEGP
                     LAGGHLGFSEEELSRAEDFSLERLLPETLDALKPFEDAFSVKIPVIAAGGIYTGHDIA
                     RMLSLGASGVQMATRFVCTRECGVSDAFKQAYLDASEDDIVIIKSPVGIPGRAVRNRF
                     LERLDRGEPIPINCPYKCLSTCKIYDSRYCIANALLNAYDGDMENGLVFAGHNVSRVH
                     EILTVKELIDSLLDELRAA"
     gene            complement(2748..>3270)
                     /locus_tag="PKW60_12350"
     CDS             complement(2748..>3270)
                     /locus_tag="PKW60_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HPK00269.1"
                     /translation="EELEHARQRGARIYAELAGYGNTADAYHITAPEPEGDGMIRVMR
                     AALNDAGLAPEGIGYINAHGTSTVLNDRTESLAIQRVFGEHAQNLKISATKSMIGHLM
                     AAAGAAEFVATVMSLHTGKIHPTINYEEPDPECPLDYVPNVAQACQFDVAMCNAFGFG
                     GGNACLIVRRFGE"
BASE COUNT          607 a          977 c         1012 g          674 t
ORIGIN      
        1 cgtcccggtc gaaggtctgc cgggccgtct ccatgatctg gtccgcctcg gcgttgttga
       61 acccgacaaa attcgacccg gtgccctcgg ccatgctcga atgccagacc tggtacgggt
      121 cgaaatctgc gggcatcgac cagcccagaa tgcaggcgtc aaacttccgc gcgtcgatct
      181 gttgaagaaa tgtggcccat tccagggggc ggagcgacat ctcgatgccg gcccgcttga
      241 gttcgttctg aaagatcgtg gcgatctgct ccgtttcggg gctgtcgttc ttgataagca
      301 ggtcaaaacg gaacacggtc ccgtctttat cgcggagtcc gtcgccgtcg gaatcgaccc
      361 agcccgcttc ggtcaggagc tgtttggcct gctcaggatc gaagggccac ggctggatgt
      421 ttttgttgta ttccggactc tcgacaaagg cgttgcccgt cacggtgatc gccaggccgt
      481 ggaagatctt ttcgcggatg tcctcgcggt tcagcagcat ggtgagtgcg cggcggaccc
      541 gcttgtcgct gaattgaggg cggcggctgt tccagccgat gtaattgtag tacggcgcat
      601 aaaacgagaa tttgttgaac tgtcccgtga accgtttgga acccgtctgg cgcacccact
      661 gttcgggagt gaggcccatc gtatcgagtt gccctcccag gagcgcctgc agcgcgggcc
      721 catcggaaag gatgatccgg tatacgcgct ttgcgaggta gggccgcttg ccccagtaga
      781 cgggattctc caccagcacc atttgctggc cgggatccca tttttccagc atgtacggcc
      841 ccgaaccaat gggttttcgg ttattggggt gggcgttgaa atcgcccaca ccgtaaatgt
      901 gcctgggtat gatgtcgagg ccgcccagca tgacgatatg gcggaaatac ggttttgagc
      961 acacaaaccg gaccgtccgc gcatcgataa cctcgcaggc cgtgacatcc tggtagtagt
     1021 tgcgcaggtg cggcgcatca accttgggat ccttgatggt gtcaaaactg aacttgatgt
     1081 cttcggtcgt cagtggcgtg ccgtcggaaa acacggtccc ttcgtgaaga gtaaacgtat
     1141 acgccagctt atcgtcggaa atttcccagg attcggccag ccgggggatc atctcgaggg
     1201 tttcgttgtc gcgttccagc aggctctcaa aaatgaacgc attgatccgc gacgcatagg
     1261 cgtcagaact cgtgtagggg ttgaggtttt cggtctcggc cgacatgcgc gtgatgagcc
     1321 agtccccgta ctcgggctcg ccgtccattt ccgccgatgc ggtggacgcg gccccggcat
     1381 cctctgtgtc aggcgctgaa ccgggctgcg cggccggacc gccgcaccca cccaggagga
     1441 gcgcccacag gaatgtcatc agtaccgccg cacgcatggg ttggagcaag acacgccgcc
     1501 accaattccg catgaaagcc tccaagactc atttttcgcc gcaaagcagg ggcgccatga
     1561 attccgcggc cgccatacta aacaaaggat tgcctcgccc gcaagcgccc tgcagccaga
     1621 acagctccgt ttcggagggg tgttgtcggc ggacggcgtc cgtacgccgc cagtgaaatc
     1681 aagcggcccg cagctcgtcg aggaggctgt cgatcagctc cttgaccgtg aggatctcat
     1741 ggacgcgcga gacgttgtgc cctgcgaaca ccagcccgtt ctccatatca ccatcgtagg
     1801 cgttgagcag cgcattggcg atacagtagc gggaatcata aattttacac gtgctgaggc
     1861 atttataggg gcagttgatc ggaatgggtt caccgcgatc cagacgttcg agaaaacggt
     1921 tgcggaccgc gcggccggga atgcccacgg gactcttgat gatgacgata tcgtcttcgg
     1981 aggcatcgag atacgcctgt ttgaacgcat cggacacgcc acattcccgg gtgcacacaa
     2041 accgggtcgc catctgaacg cccgacgccc cgagcgacag catgcgggcg atgtcgtggc
     2101 cggtgtagat gcctcctgcg gcgatcacgg ggattttgac gctgaacgcg tcttcaaacg
     2161 gtttgagggc gtccagggtc tcgggcagca gccgttcgag cgagaaatct tccgcccggg
     2221 acaactcctc ttcggagaaa ccgaggtggc cgccggccag cggaccctcg aggatgacgg
     2281 cctgggccgt acgcccgtac cgtttttccc aggtccgaag gatcagggca gcggcccggc
     2341 cggaactgac aatcggtacc aggttaacgc tgtcgtcttc gaccatggcc ggaagcttca
     2401 gcggcaatcc tgcgccgaca acgaccattt tgacgccttc gcggacactg gtctggataa
     2461 gcgtttccgc attgctcagg acgctcatga cgttcacggc gatcggtccg tgggtagcac
     2521 tccgggcctt gcggatctcc tcggcaatgg cctcgctgct gaccccctcg taatcttcgc
     2581 gcgtcgtgtg catatcgccg aggcccacgc tggcgatggt gccgatacca ccttcattgg
     2641 ccacggcggc cgccagcgag gccagcgaca ccttaacgcc catgccgccc tgaattatgg
     2701 gaacttcggc tgtaatcgag cctatctgaa gttttgggat gtccacgcta ttctccgaag
     2761 cgtcgtacga tgagacacgc gttgccgccg ccgaacccga atgcattgca catggccacg
     2821 tcgaattggc acgcctgggc cacattgggc acgtagtcca gggggcattc cggatcgggt
     2881 tcctcgtagt tgatggttgg atgaatcttg cccgtatgaa ggctcatgac ggtcgccaca
     2941 aattccgcgg cgccggccgc ggccatcaga tggccaatca tggactttgt cgcgctgatt
     3001 ttcaagtttt gggcgtgctc gccgaagacc cgctggatgg cgagagactc ggtgcggtcg
     3061 ttcaggaccg tcgacgtgcc gtgggcattg atgtagccga tgccttcggg ggcaaggccc
     3121 gcatcgttga gggccgcgcg catcacccgg atcatgccgt cgccttccgg ctcgggggcg
     3181 gtgatgtggt aggcatcggc cgtgttgccg tagcccgcca gttcggcgta aatccgtgcg
     3241 ccgcgttgcc gggcgtgctc caattcctcc
//
LOCUS       DAOPBQ010000430         3267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23434_length_3267_cov_3.318182, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000430 DAOPBQ010000000
VERSION     DAOPBQ010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23434_length_3267_cov_3.318182"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PKW60_12355"
     CDS             complement(<1..923)
                     /locus_tag="PKW60_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00270.1"
                     /translation="MTSTEVEESRPLHRWLSELAAGAFHPSLPALLGALLVAAVLFLS
                     CITLLTQDLLAEANCGYFMVDWKDDYAYLTCTVLQEARDNSPEPVIALIGASALRESI
                     AGADDLAGLMKEELGRKVRVVPLTAAGLTHWEAAAIADLVAGHMQGVVILGVSPGKMA
                     LPRGFFEDLVRQPRLALDSSAFDEEADLAGIALPRRTGNFFLDHHGFFSARPSAVLNL
                     VTGPVVPDLHQARTWRILSEDEWANAEISLLGRVGGYTENGTRNFEVMGRTIERLRGA
                     GGIQPVLLESVRNPRADQVMRDSPACREALTR"
     gene            1227..1979
                     /locus_tag="PKW60_12360"
     CDS             1227..1979
                     /locus_tag="PKW60_12360"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPK00271.1"
                     /translation="MPRSLIILSAIAIAVTGITCQSLTPLSTPPPGVYLVRQIDEPLT
                     IDGRLDEPAWQRASVFNRFFVFRPANGSFASPTVLRMLWDSEYLYLAYECEDEDIWSF
                     SDQPDDSLWNGDVVEFFVKPFRDQTFYYEFVMAPNGALFDACYPSRGAGGANRFKTWD
                     SGARIATTINGTDNNHRDDDQGYIVEIAIPWAAFREAGKPAIGTEWTFGAFRYDYSKL
                     FQDPLLLKSFPGDAPHGFHSYEKYGEMRFVSQ"
     gene            2014..>3267
                     /gene="glpK"
                     /locus_tag="PKW60_12365"
     CDS             2014..>3267
                     /gene="glpK"
                     /locus_tag="PKW60_12365"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPK00272.1"
                     /translation="MAKDFILALDQGTTSSRAILFDREGAIHASCNEEFPQIYPQPGW
                     VEHDPEAILRTQCATAKRVLHEAGIPPADVAAIGITNQRETAVVWDRNTGEPIHNAIV
                     WQCRRTADLCEQLDAAGLSGTFRERTGLVLDAYFSGTKIKWLLDHIPGARARAAKGEL
                     CFGTVDTWLLYRLTGRHATDPSNASRTLLYNIVEQRWDDELLRHLDVPAEMLPEVKPS
                     SCIFGETSAFGATIPIGGLCGDQQSALFGQTAFGHLESKNTYGTGCFLLANTGAAPVQ
                     SNHGLITTIAWDLGQGVQYALEGSIFIGGAVIQWIRDELQLISSAAESESVAGQVDDT
                     GGVYIVPAFVGLGAPHWDMHARGIIVGLTRGTNRAHIVRAALESISFQTHDIIRCMES
                     DTGAAIPLLKVDGGAAANNLLMQHQA"
BASE COUNT          661 a         1120 c          933 g          553 t
ORIGIN      
        1 cgcgtgagtg cttcccggca ggccggcgag tcccgcatga cctgatcggc acggggattt
       61 ctcacggatt ccagcagcac gggctggata ccgccggccc cccgcaaacg ttcgatcgtg
      121 cggcccatca cctcgaaatt gcgcgttccg ttctcggtat agcctcccac ccggccgagc
      181 agcgatatct ccgcgttcgc ccattcgtct tcggagagga tcctccaggt ccgggcctgg
      241 tgcaggtcag gaactaccgg gccggtcacc agattgagca cagccgaagg ccgggctgaa
      301 aagaacccgt ggtgatccag aaagaagttc cctgtgcgcc gtggcagtgc gatacccgcc
      361 agatccgctt cctcgtcaaa cgcgctggaa tccagggcca gccgcggctg ccgcaccaga
      421 tcctcgaaaa atccgcgcgg cagcgccatt ttccccggcg acactcccag gatcaccacc
      481 ccctgcatgt ggccggccac gagatcggca atggccgcgg cttcccaatg cgtgagaccc
      541 gcggcggtca ggggaaccac gcggaccttc cggccgagct cctctttcat caggccggca
      601 aggtcatccg cgcccgcgat ggattcccgc agcgcggatg ccccgatcag cgctatcacc
      661 ggctccgggg aattgtctcg cgcctcctgc aacaccgtac acgtcagata ggcgtagtcg
      721 tctttccaat ccaccatgaa atagccgcaa ttcgcttcgg ccagcagatc ttgcgtgagc
      781 agggtgatgc aggacaagaa aagaaccgcg gcaaccagca gtgcgcccag aagagcgggc
      841 aacgacggat gaaacgcgcc ggcggccagc tcgctcagcc agcggtgtaa tggtctgctt
      901 tcctcaactt ctgtagacgt caagcggttc accctggcag acaaaaacaa acccccctgg
      961 ggaatcccag cggggaattt ccgatgctct actggtaata ttatcaaaga catggccaaa
     1021 aaggcaattc gagagcgaaa ttcggtgcgc cgcgacctca attgtctccc gcaaaacgtc
     1081 ggttgcggca agcgccctgg cgctccggga cgcgtcccaa ccccaagcct gcactggtac
     1141 cacggccggg atgacgcaca ggcccgggaa cgctatgata agggccgcca ggaaaccagc
     1201 ccgcgaagta tccggggaga aatgccatgc cacgcagcct gatcatcctg tcggcgatcg
     1261 cgatcgccgt taccggcatc acgtgtcaat cgctcacacc gttgtccacg ccgccgccgg
     1321 gcgtctacct cgtccggcag atcgacgaac ccctcaccat cgatggccgt cttgacgaac
     1381 ccgcctggca gcgggccagc gtgttcaacc gcttcttcgt cttccgtccg gccaacggtt
     1441 ccttcgcgtc cccgacggtc ctgcggatgc tgtgggacag cgagtacctc tacctcgctt
     1501 atgagtgcga ggacgaggac atctggtcgt tttccgatca acccgacgat tcgctgtgga
     1561 acggcgacgt agtcgagttt tttgtgaagc cgttccgcga ccagacgttc tactatgaat
     1621 tcgtgatggc gcccaacggg gccctgtttg acgcctgcta cccgagccgc ggcgcgggcg
     1681 gggcgaaccg gttcaaaacc tgggattcgg gcgctcgcat cgccaccacc atcaacggca
     1741 ccgataacaa ccaccgggac gacgaccagg gctacatcgt ggagatcgcg atcccctggg
     1801 cggccttccg cgaggcggga aaaccggcca tcggcacgga atggacattc ggcgccttcc
     1861 gctacgacta ctccaaactg tttcaagacc cgctcctgct caaatcgttc ccgggagatg
     1921 cgccgcacgg gttccattcc tacgaaaaat acggagaaat gcgcttcgta tcccaataaa
     1981 acaccggatg ttgcaggcaa gccaggggga accatggcca aagattttat tcttgcgctg
     2041 gaccagggca ccacgtcgtc gcgggccata ctgttcgacc gcgaaggcgc catccacgct
     2101 tcatgcaacg aggaattccc gcagatctat ccccagcccg gatgggtcga gcacgacccc
     2161 gaagccatcc tgcgcacgca gtgcgccacc gcgaaaagag tcctgcatga ggccggaatc
     2221 ccgcccgccg acgtcgcggc catcggcatc accaaccagc gggaaaccgc cgtcgtgtgg
     2281 gatcggaaca cgggagaacc catccacaac gcgatcgtgt ggcaatgccg gcgcaccgcg
     2341 gacctctgtg agcagctcga cgccgcgggt ctcagcggca cattccgcga gcgcaccggc
     2401 cttgtgctcg acgcctactt ctccggcacg aaaatcaaat ggctgctgga ccatatcccc
     2461 ggcgcgcgcg cgcgggctgc caaaggcgag ctttgtttcg gcaccgtcga tacctggctg
     2521 ctctaccggc ttaccggccg ccacgctacc gaccccagca acgcctcccg aacgctcctg
     2581 tacaacattg ttgagcagcg ctgggacgac gaactccttc gccatctcga cgtgcccgcc
     2641 gagatgctcc ccgaggtgaa gccgtcgagc tgcattttcg gcgagacgtc tgcatttgga
     2701 gccacgatcc ccatcggcgg cctctgcggc gatcagcaga gcgcactctt cggccagacc
     2761 gccttcggcc accttgaaag caaaaacacc tacggcaccg gatgctttct ccttgcgaac
     2821 accggcgcgg cccccgtaca gtcaaaccac ggcctcatca caaccatcgc ctgggacctt
     2881 ggccagggcg tgcagtacgc cctggaagga agcatcttca tcggcggcgc cgtcatccag
     2941 tggattcgcg acgaactgca actgatctcc agcgcagccg aaagcgaatc cgtcgcgggg
     3001 caggtggacg acaccggcgg cgtgtatatc gtgcccgcgt tcgttggcct cggcgcgccc
     3061 cattgggaca tgcacgcgcg cgggatcatc gtgggtctca cgcgcggaac caaccgggca
     3121 catatcgtgc gcgccgcact cgaatcgatt tcttttcaaa cccacgacat catccgctgc
     3181 atggaatccg acaccggtgc ggcgatacct ctcctgaaag tcgatggcgg cgccgccgcg
     3241 aacaaccttc tgatgcaaca ccaggcc
//
LOCUS       DAOPBQ010000431         3264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23464_length_3264_cov_3.483640, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000431 DAOPBQ010000000
VERSION     DAOPBQ010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23464_length_3264_cov_3.483640"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="PKW60_12370"
     CDS             <1..1338
                     /locus_tag="PKW60_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00273.1"
                     /translation="YAASEAVIIIGFTGVGSSPTGTTSTQTTGARGRTGGQASRRGGT
                     TAATAGATGVEGTEGGITAQGGVEVLNYAPNIIVMLQRMVSPVIEPETEEVLSYMIYN
                     PTTNQLVARNTPTNLDLLEQQLEQIDLVPKQVTIEAKFVTVSVSDLDKVGFEWDVNLS
                     DVNGRARAVRGLQDTTYAYDINGDGVTEDIPFYTRPDGTSVINNTITQGLLNALANPG
                     PPGAFSFSGTITDNADGDRLGVTFDFLNSLTETELLSAPKITTLNRRRAVIRDYTSRT
                     FITDAETTVNVTEGSYLGGTSNMAVTTEIFPETFEFGITLTVTPQVCGSDLVRVWLYP
                     FVDSYVGQDTYTSRSIVDGEQIETTTTYPQIATQEIYTNALVHDGDTIVLGGMITDRS
                     TKGDERLPYLADIPLIGFFFRGKSREIAQNSLLIFITVDIVDPTGARYFEPAR"
     gene            1421..2695
                     /locus_tag="PKW60_12375"
     CDS             1421..2695
                     /locus_tag="PKW60_12375"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPK00274.1"
                     /translation="MVSQFAYDLLTTLAAGPGAAWLAMRPGYRPLLRRFAPGVPATLE
                     RAPIWLQACSMGEVNAARPILEAMAARWPAIPLLLTTSTLTGRRQAEMTAGAVPVTWF
                     PFDHRICVGRFLDRIGPRMLVLLETEVWPNAVRMTRRRGVPVAILNGRISDKHFGRYA
                     RMKSFWRPVFGALSAVCAQNAHYADRLRVLGVPESALHVTGNTKFEGVQTEFSQDEAE
                     RLRAQAGIPPHAPVLVFGSMRDGDERLASTCWAELSARLPELRMILVPRHLERVSTVC
                     AAFTEPVLLHSALRSGQKPSGERLLVVDVMGELVKFYALATAAVIGGSFYPGVEGHNP
                     LEPAALGIPTVFGPFMRNFIDPARALLESGGAVQTDAVELPEILSRLLIDEDWRARLA
                     SNGRAAVRTTQGAIARSLDVLEGLMEPPIRAV"
     gene            complement(2634..>3264)
                     /locus_tag="PKW60_12380"
     CDS             complement(2634..>3264)
                     /locus_tag="PKW60_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00275.1"
                     /translation="SESDLRFQVYSQLAFGARGLAYHRYWLTIPHDLWQAEPAAGMRR
                     SMVNPFTGERHYTWRNVAEVNREVSALWPVLRGLKVRDVFFAGDAPSGLRPLPFGKLP
                     IRAVIAQSALLSFLRDGDGRDWAFVVNTVHGMQRSAKAAERTLRLQLDPAIGEVVEVE
                     RATGKLKSTDVKRNELYLTLPGGTGTLLLLIPHGSAVPSGLPAHQESVQ"
BASE COUNT          624 a          994 c         1033 g          613 t
ORIGIN      
        1 tacgccgcat cggaagccgt cattatcatc ggcttcacgg gcgtcggcag cagcccgacc
       61 ggcaccacgt ctacccagac cactggcgcg cgcggtcgca caggcggcca ggcgagccgg
      121 cgtggcggta caaccgcggc aaccgcgggt gccaccggcg tcgaaggcac tgaaggcggc
      181 atcacggcgc aaggcggcgt agaagtcttg aattatgcgc cgaacatcat cgtgatgttg
      241 cagcgtatgg tatcgcccgt gatcgagccc gagacggaag aagtgctttc gtatatgatt
      301 tacaacccga ccactaacca gctggtggca cgcaataccc ccaccaatct ggacttgctg
      361 gagcaacaac tggaacagat cgacctggtg cccaaacagg tcaccatcga agccaaattc
      421 gtgacggtca gcgtgtctga cctggataag gtgggattcg agtgggacgt gaacctgtct
      481 gatgtgaacg gccgcgcgcg cgcagtgcgc ggtttgcagg atacgaccta tgcctacgac
      541 atcaacggcg acggcgtgac ggaagacatc ccgttctata cgcggccgga tggcacgagc
      601 gtgatcaaca ataccatcac gcaggggttg ctgaatgcac tggccaaccc cggacctccg
      661 ggcgccttca gtttctcggg aaccatcaca gacaacgcgg atggcgaccg gctgggggtt
      721 acgtttgact tcctgaacag tctgacagaa accgaactgc tcagcgcgcc caagatcacg
      781 acactgaacc ggcgccgcgc ggtgattcgc gattacacgt cgcgcacctt cattaccgac
      841 gccgagacca cggtgaacgt gacggaaggc tcctatctgg gcggaacctc gaacatggcc
      901 gtgactacgg agatcttccc ggagaccttc gagttcggca tcacgctcac ggtgacgccg
      961 caggtgtgcg gctcggatct ggtgcgtgtg tggttgtacc cgttcgtcga ttcgtacgtg
     1021 gggcaggata cctacacgag ccggagcatc gtggacggag agcagattga gaccacgacc
     1081 acctatcccc agatcgcgac tcaggagatt tatacgaacg cgctggtgca cgatggcgac
     1141 accattgtgc ttggcggcat gatcaccgac cggtccacca agggcgatga acggctcccg
     1201 tatctggccg atatcccgct gattggcttc ttcttccgtg ggaagtcgcg tgaaattgcc
     1261 cagaacagcc tgctgatatt catcaccgtg gatatcgtcg acccgaccgg ggcgcggtat
     1321 ttcgaaccgg caaggtagac atcggcgctg catgaatagc tgaaatacgg gcggccggct
     1381 tggcatgagg ccagcggccg cccgttttgc atgagagccc atggtgtcgc aattcgcata
     1441 tgacctgctg acgacgcttg ccgcggggcc cggggcggcc tggctggcca tgcggccagg
     1501 gtaccggccc ttgctgcgcc gtttcgctcc cggcgttccg gccacgctgg aacgggcgcc
     1561 catctggctg caggcgtgca gcatgggcga agtcaatgca gcccggccca tacttgaggc
     1621 gatggcagcg cgctggccgg ctataccgct ccttttgact acatcgaccc tgactggccg
     1681 acgccaggcg gagatgacgg caggcgcggt tcccgtgact tggtttccct ttgatcaccg
     1741 gatctgcgtc ggccgcttcc tggaccgcat tggcccgcgc atgctggtac tcctcgaaac
     1801 ggaagtgtgg cccaatgcgg tgcgcatgac acgccggcgc ggtgtgccgg tggcgattct
     1861 gaacgggcgg atcagtgata agcatttcgg ccgctacgcc cggatgaaga gcttctggcg
     1921 gccggtgttc ggagcgcttt cggccgtatg cgctcaaaac gcacactatg cggatcgcct
     1981 tcgtgtgctc ggcgtgccgg aatcagcgct tcatgtcacc ggcaatacca aatttgaggg
     2041 cgtgcagact gaattttcac aggacgaggc agagaggctt cgcgcgcagg cgggcattcc
     2101 tccgcatgct cccgtgctcg tgtttggaag catgcgcgac ggcgatgagc gcctggcttc
     2161 gacatgctgg gccgaattga gcgcgcggct tcccgagctg cgcatgattt tagtcccgcg
     2221 ccatcttgag cgggtgagca cggtctgtgc ggccttcacg gagccggtgc tgctgcattc
     2281 tgcgcttcgg agcggccaga aaccgtcggg cgaacgcctg cttgtcgttg atgtcatggg
     2341 tgaactcgtg aagttctatg ccctggccac ggccgcggtg atcgggggca gcttctatcc
     2401 cggcgtggaa gggcacaacc cgctcgaacc cgcggcgctg ggcattccca ctgtcttcgg
     2461 cccgttcatg cgtaatttca tcgatccggc gcgcgcgttg ctggaaagcg gcggggcggt
     2521 tcaaaccgat gccgtcgagt tgccggaaat tctgtccagg cttcttatag acgaggactg
     2581 gcgggcccgg ctcgcctcaa atggacgcgc ggcggtgcga accacacaag gagctattgc
     2641 acgctctctt gatgtgctgg aaggcctgat ggaaccgccg atccgtgcgg tataagtaaa
     2701 agcagcgtcc ccgtgccgcc gggaagcgtc agatacagtt cgtttcgctt gacgtctgta
     2761 gatttcagct taccggtggc ccgttccact tccacgacct cgccgatggc tggatcgagc
     2821 tggagccgca acgtgcgttc ggctgctttc gcgcttcgct gcatgccatg aaccgtattt
     2881 accacgaacg cccagtcgcg gccatcgcca tcgcgcagga agctcaagag agcggattgg
     2941 gcgataacgg cccggatggg cagcttaccg aaaggaagag ggcgcagccc cgaaggggcg
     3001 tcgccagcga aaaacacgtc tctgaccttc agcccgcgca gaaccggcca caacgcgctc
     3061 acttcccggt tcacctccgc gacatttctc cacgtatagt gccgctcgcc ggtaaaggga
     3121 ttcaccatac tgcggcgcat cccggcggca ggctcggcct gccacagatc atgagggatg
     3181 gtaagccagt agcggtggta cgcgaggcca cgggcgccaa aggcgagctg gctgtagacc
     3241 tgaaagcgca ggtcgctctc cgat
//
LOCUS       DAOPBQ010000432         3262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23479_length_3262_cov_3.546305, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000432 DAOPBQ010000000
VERSION     DAOPBQ010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23479_length_3262_cov_3.546305"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PKW60_12385"
     CDS             complement(<1..883)
                     /locus_tag="PKW60_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HPK00276.1"
                     /translation="MRLLICTLVACALLGTAYADLQNVQVYGRIDIRARYYRNSFNNA
                     AVLGGGPALETRIPAFLLPGRATGQAAGVAGLFDYDHARSDWSFTELVTTLGVKADFT
                     NNVNAVIEFYDFAWWGEDFRSDYITGADSRATTSDDVELLQSYIEMNEVGGLPLRLRI
                     GRQRLRYDEGWLLSDRATPTLRIAWDGVLVTYATDQFEVDAFATKLAENSPFEQDGDI
                     DFYGIRAAYTALENVDFAAWWYWVRDARSFSDTNLTWFGEWMENWFGVDDYDPTNLHT
                     VGGRVWGNWGALDYDLKL"
     gene            complement(1005..2321)
                     /locus_tag="PKW60_12390"
     CDS             complement(1005..2321)
                     /locus_tag="PKW60_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPK00277.1"
                     /translation="MAHVIPERIWDPGAETMPSGDLRALQLARLQETVARVANVPFYK
                     AEFARLGIGPDSIKSLDDIRRLPFTTKEDLRQQYPLGFLAVPRSAVARYHGSSGTTGK
                     PTFVAYTARDLQIWSDLCSRFLVAGGLKPHHTVHVAFGYGLFTGGFGLHYGIERVGAA
                     IVPASSGNTPRQILLIQDLGAEVLISTPSYALQIAEVAREQGLKPSDLPLQYAHFGGE
                     PWTEDMRLEIETQLGIEAYNNYGLSEVIGPGVSGECHLRCGMHVQEDHFIVECVDPET
                     LEPLTDGSYGELVITALTKEAFPLLRYRTRDIAALDVCPCDCGRTSVRMSRIVGRTDD
                     MLIIRGVNVFPSQIEEALLRVEGTAPHYEIVIERPAALDEVTVRVEVRPGLFSDSMRE
                     MQLLREKLVREIVSVTGIRARVELISPQTLQRSPGKARRIVDLRNA"
     gene            2708..>3262
                     /locus_tag="PKW60_12395"
     CDS             2708..>3262
                     /locus_tag="PKW60_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00278.1"
                     /translation="MRYVVWVAGVLLAALEGGPDAATHPGEPISAIPLQSVSVTANTG
                     EKPQSKLWCHDGRWWAVLPESSGTRLWRLDGAQWSPVLHLSDATATHADALRADGLVH
                     VLLFETGRCELISLACDAESHTYGLWPERTSGTVLTLNSSVETATIDMDASGRLWLAM
                     DDEAKVVVRWSDAPIRPAYTAGSHS"
BASE COUNT          606 a          938 c         1077 g          641 t
ORIGIN      
        1 caagtttgag gtcgtaatcc agggcgcccc agttgcccca tacgcggccg ccaaccgtgt
       61 gcaggttggt tgggtcataa tcgtccacgc cgaaccagtt ttccatccat tcgccaaacc
      121 aggtcaggtt ggtgtcggaa aatgagcggg cgtcgcgtac ccagtaccac catgcggcga
      181 aatcgacatt ttcgagggcg gtgtaggcgg cacggatgcc gtagaagtcg atgtcgccgt
      241 cctgctcaaa cgggctgttc tcggcgagct tggttgcgaa cgcgtccacc tcgaactggt
      301 cggtggcata ggtgaccaag acgccgtccc atgcgatccg caaggtgggc gtggcgcggt
      361 cgctgaggag ccagccttcg tcgtagcgca gccgctggcg tccgatgcgc aggcgcaggg
      421 gcaagccgcc gacttcattc atttcgatat acgactggag cagttccacg tcgtccgacg
      481 tagtggcgcg gctgtccgca ccggtaatgt aatcgctgcg gaaatcttcg ccccaccacg
      541 cgaagtcgta gaattcgatc acggcgttca cattgttcgt gaaatcggct ttgacgccga
      601 gggtggtcac caactccgtg aaggaccagt ccgagcgggc gtggtcgtaa tcgaacagac
      661 cggcgacgcc ggcggcctgg ccggtggccc gtccgggcag gaggaacgcc ggtatgcggg
      721 tttcgagtgc ggggccgccg cccagaactg ccgcgttgtt gaaggaattc cggtaatacc
      781 gcgcgcgaat gtcgatgcgt ccgtagacct gaacattctg taaatcggcg tacgcagtgc
      841 ccagcagggc gcacgccacc agcgtacaaa tcagtagtct catggtttcc ccctcgtgtt
      901 gatgttctgc cttccgcctc atgcttcgag agcgtgatct acccggtccc ctctcaggca
      961 tcccccgagt catccctttt ttatacacga gttcatccga aaagtcacgc atttcggaga
     1021 tcgacaattc gcctggcctt cccggggctg cgctgaaggg tctgcgggct gatcaattcg
     1081 acgcgggcgc gtataccggt gaccgacaca atttctctaa ccaatttctc acgaaggagt
     1141 tgcatttcgc gcatggagtc ggagaagagc cccggacgca cctctacgcg cacggtaacc
     1201 tcgtcgagcg cggcgggccg ttcgatgacg atttcgtagt ggggggcggt gccttccacg
     1261 cgcagcagcg cttcttcgat ctgggatggg aatacattga caccgcgaat gatgagcatg
     1321 tcgtccgtgc ggccgacgat ccggctcatc cggacagagg tccgcccgca gtcgcagggg
     1381 catacatcga gggccgcgat atctcgcgtg cggtagcgca acagcgggaa ggcttccttg
     1441 gtgagcgcgg tgatcaccag ttctccgtag ctgccgtcgg tgagcggttc gagcgtctct
     1501 ggatccacgc attccacaat aaagtggtct tcctggacgt gcatgccgca gcgaaggtgg
     1561 cattcgccac tgacaccggg cccgatgacc tcgctcaacc cgtaattgtt gtaggcctca
     1621 ataccgagct gcgtctcgat ttcgagtcgc atgtcttcgg tccacggttc gccgccgaag
     1681 tgcgcgtact ggagcggaag atcggagggt ttgaggcctt gttcccgggc aacttccgcg
     1741 atttgcaggg cgtagctcgg cgtggagatc aagacttcgg cgccgagatc ctggatgagg
     1801 agaatctggc gcggtgtgtt tccggacgac gccgggacga tggccgcgcc cacacgctcg
     1861 atgccgtaat gcagcccgaa gccgccggta aacagtccgt agccgaaggc aacgtgcacc
     1921 gtgtggtggg gtttcaggcc gccggcgacc aggaagcgtg agcagaggtc ggaccatatt
     1981 tgcaggtctc tggcggtgta ggccacgaac gtgggtttac cggtcgtgcc ggaggaccca
     2041 tggtagcgtg ccaccgcgct tcgcggtacg gcgagaaagc ccaggggata ctgctggcgc
     2101 aggtcctctt tcgttgtgaa aggcagccgc cggatgtcgt cgagcgactt gatgctgtct
     2161 gggccgatgc ccaggcgcgc gaactcggcc ttgtaaaagg ggacattggc gacgcgcgcg
     2221 acggtttcct gaaggcgcgc aagctgcaga gccctgagat cgccgctggg catggtttct
     2281 gcgccgggat cccaaatccg ttctggaata acgtgtgcca taagctaatc cacgagaggg
     2341 tgcgcaagag acataatcct gccgtccccc gagtcctgtg ttggtcccgg cggcacccgg
     2401 aacggcgtgc cgcgggcgca tgcgtcctgt agagcggagc agtaccacac cacatgccgt
     2461 cctccaatgt caagaaaacg gtttaaggcg aaatccgtcg tcagggccgg cggggctgcg
     2521 cggcacggaa agacgcgtgt ttttaaagca atttgtcgca gattgccggc aggggggagt
     2581 gatgcaccgc acggccctgg gtgacgtgtc cgtagccggt ttcagtatac tgttggggag
     2641 cgggcatgtt gtcacgagaa atcagtgcag gtgtcacgta gagagtcggc ccgcgggaag
     2701 gagaaaaatg cggtatgttg tatgggtagc gggggttttg ctggcagccc tggaaggcgg
     2761 gcctgacgcg gcgacgcatc cgggcgagcc gatttctgcc attcccctgc aaagtgtgtc
     2821 tgtgacggcg aatacggggg agaaacctca gtccaaacta tggtgccacg acggtcgctg
     2881 gtgggccgtg ctgccggaaa gcagtggtac caggctgtgg cgcctggacg gtgcgcagtg
     2941 gagcccggtg ctgcacctgt cggatgccac agcgacccat gcggacgcgc tccgcgcgga
     3001 cggactggtg catgtgctct tgtttgaaac cgggcgctgt gagctgatct cgctggcctg
     3061 cgacgccgag tctcacacct atggactctg gccggagcgc acgtctggca ccgtgttgac
     3121 tctcaactcc agtgtagaaa cggcgaccat tgatatggat gcttcgggcc gcctgtggct
     3181 ggcaatggat gatgaggcga aagttgtcgt gcggtggagc gacgcgccta tccgcccggc
     3241 ttacacggca ggcagtcaca gt
//
LOCUS       DAOPBQ010000433         3260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23498_length_3260_cov_3.810608, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000433 DAOPBQ010000000
VERSION     DAOPBQ010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23498_length_3260_cov_3.810608"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKW60_12400"
     CDS             <1..320
                     /locus_tag="PKW60_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00279.1"
                     /translation="PTPAEGVVSSTRPSGPGSHRALLASSPHFAAEHIVCAGAYERET
                     QGESFHILLAISGSLAVNAGFDMHSLAPGEALLVPGLHQRYFVEGHGEFLDYYVPGAS
                     PVL"
     gene            632..2677
                     /locus_tag="PKW60_12405"
     CDS             632..2677
                     /locus_tag="PKW60_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPK00280.1"
                     /translation="MRLRTVASLLMFVFLMFMAFFSGQASLAAEIPDPLRNSVDTAVA
                     LVKPALVRIEVVTPSYYEGREVKYESSGSGVIVTPEGHVVTNHHVAGRATRMLCTLST
                     KEKIEARLVGRDPLSDIALLKLVPEAPRQFPVASWGDSTEVKVGDQVLAMGSPMALSQ
                     SVTLGIISNTEMIMPRWAGRLDEDGEDVGSFVVWLGHDAQIYGGNSGGPLVNLRGEII
                     GINEIRVGGLGGAIPSELARTVVSSLLEKGEVERAWLGFDIQPRFKYSPMKQGALIGN
                     VMKATPAGDAGLKAGDYLLRLAGQEIDVAFDEQIPLFNRFVAGLPIGEPVQMAFSRDG
                     VEQTISMTPAKRHEASPTQSELRQWGLTVQDISFLKARELRRESQDGVIITSVRPGGP
                     AGDAKPALEEKDVITAVAGLPVKEVEELKQITADQTEGKTEPAPVLVQFERREQQLVT
                     VVEVGIQELKDPGLEVKKAWLPIETQVITRDIARNLGDETLTGFRITRIYTGSTAQKA
                     GLLVGDLILAVDGMELEADAPEDYEELPVLIRQYKVGTSAELSIQRNGERKTISVELV
                     ASPKLDREMKKYRDSNFEFTVRDITFFDRAREQWEENKTGVLVEEIKSGSWAALGLLG
                     VSDLITEVSGEKVADVDWFEQKMDAVAAEKPKTVVFKVMRGIYTLFLQFEPQWEGGV"
     gene            2750..>3260
                     /locus_tag="PKW60_12410"
     CDS             2750..>3260
                     /locus_tag="PKW60_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00281.1"
                     /translation="MRSGYLGTGLCLALLSIVTGAERAQADTLADAARAVVEVNKQAV
                     VTIKLVVENRYSMEGSGSQSEESTSEITGTVIDPSGLTVVSLFTTDPTSASRNMMDMG
                     LEEMGFKIETEIKRAGILAADGKEIPAEVVLRDKDLDLAFLRPKQPPEKPFAALELAQ
                     AGSLSQLDPI"
BASE COUNT          705 a          929 c          986 g          640 t
ORIGIN      
        1 gcccaactcc tgcggaaggg gtggtttcgt ccacacgtcc ctctggcccg ggcagccata
       61 gagccctgct ggcgtcttcg ccccattttg ccgcggaaca catcgtctgt gctggggcat
      121 acgagcgcga aacccagggt gagtcgttcc acattctcct ggccatatcg ggttccctgg
      181 ccgtaaacgc aggttttgat atgcactccc tcgctccggg cgaagctctt ctcgtacccg
      241 gacttcatca acgatatttt gtcgagggtc acggggaatt tttggactac tacgtccccg
      301 gcgccagtcc ggttctgtag agcgttacgg tgctggaaaa gggcctgtgc ctcacgaatg
      361 aaacagccgc gctttccagg aggtggatgc tgaaacgaag ggaaggcgga tcaggcaaaa
      421 ccgaagccat tttcccgtga attagcgtcc gttttcggta ttccttgaca cgcctggacg
      481 ccttaatcat aatcgccgtg tggatgcggg cggccagggc atgtgctacc tccagccaac
      541 cgttccgatg gcttccagct ggatacagtg gccccgccaa tgacccgcgc acacttctaa
      601 aacgaaagcc gcgacgtata aggaaacgcc catgagactt cgtaccgttg ccagtctgct
      661 gatgttcgtt ttcttgatgt tcatggcctt cttctccggg caggcaagcc tggcggcgga
      721 gatccctgat cctctgcgta attccgttga caccgcggtc gctctggtta aaccagcact
      781 ggtgcgtatt gaggtcgtca cgcccagcta ctacgaaggg cgcgaggtaa aatatgaatc
      841 gtccggcagc ggcgtcattg tgaccccgga ggggcatgtc gtcaccaacc atcatgtggc
      901 gggccgcgcc acccggatgc tctgcacctt gtcgacaaaa gagaaaatcg aggcccgcct
      961 tgtcgggcgc gaccccttgt cagacattgc cttgctgaag ctcgttcccg aagcgccgcg
     1021 gcagtttccc gtggcttcct ggggcgattc cacagaagtg aaggtggggg accaggtact
     1081 ggccatgggc agccccatgg ccctgtcgca gtccgtcaca ctgggcatta tcagcaacac
     1141 cgagatgatc atgccgcgct gggcaggccg cctggacgag gatggcgaag acgtgggctc
     1201 gtttgtcgtt tggctgggcc acgacgcgca gatctacggc ggcaacagcg gcgggcctct
     1261 ggtcaatctg cggggtgaga tcatcggtat caatgagatt cgcgtgggcg gcctgggcgg
     1321 cgccattccc agtgagctcg cccgaacggt cgtgtcgagt ttgctcgaaa aaggcgaagt
     1381 ggagcgcgcc tggctcggat ttgatattca gccccggttc aaatacagcc cgatgaagca
     1441 gggcgcgctc atcggcaatg tcatgaaagc gaccccggca ggagatgccg gattgaaggc
     1501 tggagactac cttctgcggc tcgccggcca ggaaatcgac gtggcgtttg atgagcagat
     1561 cccgcttttt aaccgttttg tggccggcct gcccatcggt gaaccggtgc aaatggcttt
     1621 ctcacgcgac ggagtcgagc agaccatttc gatgacgccc gccaaacgcc atgaggcctc
     1681 accaacacaa tccgaactca gacaatgggg cctcacggtg caggatatct cattcctgaa
     1741 agcccgggaa ttgcggcgcg aaagccagga cggcgtgatc atcacctctg tgcgcccagg
     1801 cggacctgcc ggcgacgcca aaccggcact cgaagaaaaa gacgtcatta ctgctgttgc
     1861 gggcctgccc gtaaaagagg tcgaagaact gaaacaaatc accgcggatc aaaccgaagg
     1921 gaagaccgaa cctgcgccgg tgcttgtcca gttcgagcgg cgcgaacaac agctcgtaac
     1981 cgtggtcgaa gttggaatac aggagctcaa agatcccggt ctggaagtca agaaagcctg
     2041 gctgccgatc gagacccagg tgatcacgcg ggatatcgcc cgcaatctgg gcgatgagac
     2101 tctgacgggg ttccgcatca cccgcatcta tacgggcagc accgcccaaa aggccggttt
     2161 gctcgtgggc gacctgattc tcgccgtgga tggcatggaa ctcgaagccg atgcgcccga
     2221 agattacgag gaactccccg ttttgatccg ccaatacaag gtgggaacgt cagccgaact
     2281 ctcaatccag cgcaacggag aacggaaaac catttccgtc gaactggtcg cctcaccaaa
     2341 gctggatcgc gaaatgaaga aataccgcga ttctaacttt gaatttactg ttcgcgatat
     2401 cacgtttttc gatcgcgccc gcgaacaatg ggaagaaaat aagaccgggg ttctcgtgga
     2461 ggagatcaag tcgggaagct gggcggccct gggcctgctg ggcgtgtccg acctgatcac
     2521 agaggtcagt ggcgagaaag tcgcggatgt tgactggttc gagcaaaaaa tggatgcggt
     2581 cgcggcggaa aagcctaaga cggtcgtgtt caaggtcatg cggggaattt ataccctctt
     2641 cctgcagttc gaaccgcagt gggaaggggg cgtctgaggg cgctccctca acgcttcatt
     2701 cggcgcggga tcgcccgcat tagcgatagc acacggaagg agaatgaaga tgcgatccgg
     2761 gtacctgggg acgggactgt gccttgcgct tctatcgatc gtgaccggcg ctgaaagggc
     2821 gcaggccgat acgttggccg atgcagcgcg cgctgttgtt gaagtcaaca agcaggctgt
     2881 ggtcaccatc aagctggtgg tcgaaaaccg ctattccatg gaaggcagtg gttcacaaag
     2941 cgaagagagc acttccgaga tcacggggac ggtcatcgat ccctcgggat taacggtcgt
     3001 cagccttttc accacggatc cgacctccgc ttcacgcaac atgatggata tggggctcga
     3061 ggaaatgggg ttcaagattg aaaccgagat caagcgggcc ggtattctcg ccgcggatgg
     3121 caaggaaatt cccgccgagg ttgtgcttcg cgacaaagac ctggatctcg ccttcctgcg
     3181 ccccaaacag ccgcccgaaa aaccgtttgc cgctctggag ctggcgcagg ctggctcgct
     3241 cagccaattg gaccccattg
//
LOCUS       DAOPBQ010000434         3250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23597_length_3250_cov_3.086072, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000434 DAOPBQ010000000
VERSION     DAOPBQ010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23597_length_3250_cov_3.086072"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PKW60_12415"
     CDS             complement(<1..672)
                     /locus_tag="PKW60_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00282.1"
                     /translation="MRPLATTSLILMCCAGTATAAPLSVQFDDDGMITVNGRRTVIIG
                     SYYPAQSDRPYADMAEAGFNLVRTGSKQEEMDKALAAGLMTWTSVGTVDLENREASTT
                     KLLDAVNAIKEHPALAFIESVDEPAWIWNEAGARIPPQPFAEAYPLIKAADPNHLLYM
                     NHAPTNLIKTMQAYNAGTDIVACDIYPVNPGGLRPMYALFEDGHQGDLNNLTISQVGD
                     YVDKMR"
     gene            complement(669..1229)
                     /locus_tag="PKW60_12420"
     CDS             complement(669..1229)
                     /locus_tag="PKW60_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HPK00283.1"
                     /translation="MLNRASVALVVIDLQESLMSRIPAAPGIVKETIRLIRFARILGL
                     PVLWTEQYPAGLGPTIPELAGELTGFQPIEKLAFGCMAEPSFVAALQETGRTSLLITG
                     VETHICVLQTALGAVERGFEVWVVADAVGARKTQQHDAALNRIVDSACQLVTAEMAIF
                     EILERAGTPEFKQCLPLLKGQGGEIS"
     gene            complement(1460..2014)
                     /locus_tag="PKW60_12425"
     CDS             complement(1460..2014)
                     /locus_tag="PKW60_12425"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HPK00284.1"
                     /translation="MSAGVESNTQETDGRASQALSSSSSHATGVGTSPAADSEQRTRH
                     GERLTHGAKVKRGQLNAVQQGRYGTGPAPYGYRRGREGENPLEVDDAEAEVVRLIFRE
                     YLNTRSTGKVVEYLLSKGISTRKGNSWSRQAISIILSNRTYRGRVSYGDVETQGLHDA
                     IIEPALFYKANALRERKRRNRGRK"
     gene            complement(2439..2879)
                     /locus_tag="PKW60_12430"
     CDS             complement(2439..2879)
                     /locus_tag="PKW60_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPK00285.1"
                     /translation="MLLSIDINSNVAVYVQIENQVQFAIASGKLQAGDQLPSVRELSE
                     RLSVNPNTVAKAYRDLEVMGLLYTRRGMGVFVNRGIENKCREECRRRTIERMHEVVAE
                     AKAAGMEREELFEVLTRSYDVDAEPYAETPPEVVALAKLRNRDN"
     gene            3059..>3250
                     /locus_tag="PKW60_12435"
     CDS             3059..>3250
                     /locus_tag="PKW60_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00286.1"
                     /translation="MQVITFRTRLFLATFSVLYHISSMSQASFLDVCAALTVSMGQNA
                     PSGQIHVDLSNVMGRQLPGK"
BASE COUNT          619 a          938 c          966 g          727 t
ORIGIN      
        1 gcgcattttg tccacataat cacccacctg gctgatggtc aggttgttga gatcgccctg
       61 gtggccgtct tcgaaaaggg cgtacatggg gcgaagtccg ccgggattga cggggtagat
      121 gtcgcaggcg acgatatcgg tgccggcgtt gtaggcctgc atggtcttga tcaggttggt
      181 gggggcgtgg ttcatataga ggagatgatt cgggtcggcg gccttgatca gcggatacgc
      241 ctcagcgaac ggctgcgggg ggattcgtgc cccggcttca ttccagatcc acgcaggctc
      301 gtccacgctc tcgataaacg ccagcgcggg atgctctttg atcgcgttca cggcgtcgag
      361 cagttttgtt gtgctggcct cgcggttctc caggtcgacg gtgccgacgc tggtccaggt
      421 catgaggccg gccgcgagcg ccttgtccat ttcttcctgt ttgctgccgg ttcgcacgag
      481 attgaagccg gcttcagcca tatcggcata gggccggtcg gattgagcgg gataatacga
      541 gccgatgatc accgtgcgcc ggccgtttac cgtgatcatg ccgtcgtcgt caaactgaac
      601 ggacaagggc gccgccgtgg cggttccggc gcagcacatc aaaatcaggc tggttgttgc
      661 aagcggtctc atgagatttc tcctccctgt cccttcagaa gcggaagaca ctgtttgaac
      721 tcgggcgtgc cggctctctc gaggatttcg aagatggcca tttccgccgt cacgagctgg
      781 caggcgctgt ctacaatacg gttgagggcg gcgtcatgct gctgcgtctt tcgtgcgcca
      841 acggcatcgg ccactaccca gacttcgaat ccgcgctcga cggctcccag ggcggtctgg
      901 agaacgcaga tatgggtctc gactcctgtg ataagcaggc tggtgcggcc cgtttcctga
      961 agggccgcca cgaacgaagg ttccgccatg cagccgaagg ccagtttttc gatgggctgg
     1021 aaccccgtga gctctccggc gagctctgga atggttgggc cgagccccgc cggatactgc
     1081 tccgtccaca ggacaggcag ccccaggatc ctggcaaacc ggataaggcg gatggtctct
     1141 ttcacaatac cgggagctgc aggaatcctg gacatcagtg attcctgcaa gtctatgacc
     1201 accagggcta ccgaagcacg gttcaacatt cagacctgcc ttttcggttg tgcaatctga
     1261 ggcggcggca cgtgggccgt gccgttttgt cttctgcgaa catggccgcc gtgaagagcc
     1321 gctcacgcgc gcggcaatta tagccaaact ctgctccccg gagacaaccc gggcggcatg
     1381 aagaaacccg tgaatgcagt tcgggcgccc tgagatgtcc tgggcgcccg gaaacaagag
     1441 cggattgaaa gcaagaaggc tatttgcggc cgcggttgcg gcgcttccgt tcgcgaagtg
     1501 cgttcgcttt gtaaaacagg gccggttcga taatggcatc gtgcagtccc tgcgtttcga
     1561 catcgccgta gctgacacgg ccgcggtacg tacggttcga caggataatc gagatggcct
     1621 gccgcgacca gctgttgcct ttgcgtgtgc tgatgccttt ggaaagaagg tattcgacga
     1681 ccttgccggt actgcgcgta ttaagatatt cccggaagat gaggcgcacg acctcggctt
     1741 ccgcgtcatc gacctcgagt gggttttctc cctctctgcc gcgccggtaa ccgtagggtg
     1801 ccgggcccgt gccataacgg ccctgctgca ccgcattgag ttggccgcgc ttgaccttcg
     1861 cgccgtgggt cagccgttcg ccatggcgcg tgcgctgctc tgaatccgcg gccggtgagg
     1921 tgccgacgcc cgttgcatgc gatgacgatg acgataatgc ctgcgaagcc ctgccgtccg
     1981 tctcctgcgt attactctct acacccgcac tcataatgac gttccatcct gtttttagac
     2041 ccgtttgtat acgtcaaaac acaaatgcga tttacatcca taaagccagt cggtcaacgc
     2101 gccaaatgca gctgtacccg aaatctcctt ccgcaccgtg gaaatccggg cttccaacta
     2161 agattctacg cgcattcggc gcttatgtca actgttttct tgagaaggga aattcctcga
     2221 gcggcaggag aaccggtaag gctaaagaca agaacagtag ccacataggg gcttcggatc
     2281 gaagatgggt tggccggaat gtggggacat caattgacag tcatgtggcc tttcgcagga
     2341 atttctcttg cgttttggct gccgggggtg tacgaaccat actccggggc cgccgccacg
     2401 cggcggcacc cggagtgctg atcagcagtg acgggacgtc aattgtcgcg attgcggagt
     2461 ttggccagtg cgaccacttc gggcggagtc tcggcgtagg gctcggcgtc cacatcgtag
     2521 ctgcgcgtga ggacttcgaa aagctcctcg cgctccatgc cggctgcctt ggcttcggcc
     2581 acgacttcgt gcattcgctc gatggtccgg cggcggcact cctcgcggca cttgttctcg
     2641 atgcctctgt ttacaaagac gcccatgccg cggcgggtgt agagcaggcc catgacttca
     2701 aggtctcgat aggccttggc tacggtgttg gggtttacgc tcaaccgctc agacagctca
     2761 cggaccgagg gcaattggtc accggcctgc aatttgcccg atgcaatggc aaactgaacc
     2821 tggttctcaa tctgcacgta aacggccacg ttgctgttaa tgtcaatact caatagcatc
     2881 tcaacacacc tcctgaactc gttaccattt cccctgcctg tgttgtctca caccagactc
     2941 tcgggatgat tcaagccggt tttcagaaaa gacaggagtt cctgaatcta ctgtaagccg
     3001 tgtataatgg ccggacacaa cccgctaacc taccggctac cggcgaggga gattcgacgt
     3061 gcaggttatt acgttccgga cacggctatt tttggccacc ttctccgtat tgtatcacat
     3121 atcctccatg agtcaagcct catttctgga tgtctgcgct gcgcttacag ttagtatggg
     3181 gcagaatgcc ccgtcaggtc agattcatgt cgatcttagc aacgtcatgg gccgccagct
     3241 tccgggcaag
//
LOCUS       DAOPBQ010000435         3249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23602_length_3249_cov_3.979962, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000435 DAOPBQ010000000
VERSION     DAOPBQ010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23602_length_3249_cov_3.979962"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKW60_12440"
     CDS             complement(<1..209)
                     /locus_tag="PKW60_12440"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK00287.1"
                     /translation="MNVLDLFSLKGKVALATGGAGLYGRQIVEALAEAGAKTFTAARN
                     RDALEKVAAALRDRGLDVTALQYDQ"
     gene            complement(206..1018)
                     /locus_tag="PKW60_12445"
     CDS             complement(206..1018)
                     /locus_tag="PKW60_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPK00288.1"
                     /translation="MRMRPSRVLKKLRRGGIVSCTKLNLYDARAAEIAAMFDFDCLWL
                     GMEHGPNDLSDIEKQIYAAKAYDVDVVVRVARGSYSDHIRPLELDAAGIMVPHVMSLE
                     DARNIVRMTRFHPVGRRPVDGGNADGKYCNVPFVDYLQQANEERFVIIQIEDPEPLDE
                     LDEICALDGIDIILFGPGDFSHGIGVPGQFDHPELLAARRRVAECANRHGKVAGTVGS
                     LGNLQELIDLGFRFVNLGADVLALSQYYQGLAAGFREHEDRYLRSRAEGGSS"
     gene            complement(1055..2017)
                     /locus_tag="PKW60_12450"
     CDS             complement(1055..2017)
                     /locus_tag="PKW60_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007253696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00289.1"
                     /translation="MKAFNGFGDKELRLAMLGMVDGNGHPYSWSAIFNGYEPGEMAKC
                     PFPAIPDYLSKEPEANLSIPGAKVTHVWTDDPADAPKVAAASRIPNVVERAEDVIGHV
                     DAVIVATDKGHEHVERCRPFVEAGLPVFVDKPLVDNEPDLAQFRRWIVEEERPILSSS
                     SMRYCKEYQPYRASTHNLGALRYVTITTPKTWERYGIHALEGMYPILGPGFVSVRNTG
                     TPERNVVHVKHERGVDVVVVATADMFGGFGDLLLCGTRASAYVKIMDSFYSFKAQLAA
                     FVDFLRSGVYPFPFSETEELMKIVIAGIRSREQSGREMPLAEIA"
     gene            2337..>3249
                     /gene="aroB"
                     /locus_tag="PKW60_12455"
     CDS             2337..>3249
                     /gene="aroB"
                     /locus_tag="PKW60_12455"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPK00290.1"
                     /translation="METLTLALGDRGYPIHIGEHALRQLPAELARLEARGAVAVCTDA
                     NVARIHGESVLESVRSAGVTPVLCVMPAGEEAKQLARIEEFCGMFLEAGLDRSSVVVA
                     LGGGVPGDVAGFAAASFMRGIRFVQIPTTIVAQVDSSVGGKTGVNHALGKNTIGAFHQ
                     PSAVIIDLTFLKTLPDRELRAGLAEVIKHGVIADAALFEYLECHVEPILAKDLDALML
                     PVRRSCEIKAAVVAEDEKEHGLRAILNYGHTFGHGIEAVTRYGVFLHGEAVALGMEAA
                     ASLARQLGLLDEEAAARQRACLESYGLP"
BASE COUNT          593 a         1064 c          981 g          611 t
ORIGIN      
        1 ctctggtcat actgcagcgc ggttacatcg aggccccggt cgcgaagagc cgcggccacc
       61 ttctcgaggg cgtcgcggtt tcgtgccgcg gtgaacgttt ttgcgcccgc ctccgcgagc
      121 gcttccacga tctgacgccc gtacagcccg gcgccgccgg tggccagggc cactttgcct
      181 ttcagcgaaa acaggtccag cacgttcatg acgagcctcc ctctgcgcgc gagcgcaggt
      241 atcggtcttc gtgttcgcgg aatcccgccg caagcccctg gtaatactgg ctcaacgcga
      301 gcacatcggc gccaaggttc acgaaccgga accccaggtc gatgagctcc tgcagattgc
      361 ccaggctgcc cacggtgccc gcgaccttgc cgtgccggtt ggcgcactcg gccacacgcc
      421 ggcgggccgc aagcagctcg ggatggtcga attgccccgg cactccgatg ccatggctga
      481 aatcgcccgg cccgaacaga atgatgtcga tcccgtccag ggcgcagatc tcgtcaagct
      541 cgtcgagcgg ctcggggtcc tcgatctgta tgatgacaaa gcgctcctcg ttggcctgct
      601 ggaggtagtc gacgaacgga acattgcaat attttccgtc ggcattcccg ccatcgaccg
      661 gacgccgccc cacgggatga aaccgggtca tgcgcacgat gttcctggcg tcttcgaggc
      721 tcatcacatg gggtaccatg atgcccgcgg catccagttc cagaggccgg atgtgatcgc
      781 tgtagctgcc gcgggcaacc cggaccacca cgtccacgtc gtaggccttg gccgcgtaga
      841 tctgcttctc gatgtccgac aagtcgttcg gcccgtgttc cattcccagc cagaggcaat
      901 cgaaatcgaa catcgcagcg atctcggcgg cgcgcgcatc gtacaggttc aatttcgtac
      961 acgagacgat gcccccacgg cggagtttct tcaacacacg gcttggtcgc atgcgcatgc
     1021 tcagttcctt tcacgcgcgg ggctgcaccc cggttcacgc tatctctgcg agtggcatct
     1081 cgcggccgct ctgctcgcgg ctgcggatgc ccgcgatcac gatcttcatc aactcttccg
     1141 tctcggagaa ggggaaagga tagacccccg aacgcaggaa atccacgaat gcggcgagct
     1201 gtgccttgaa actgtaaaac gagtccataa tcttgacgta cgccgaagcg cgggtcccgc
     1261 acagcaggag gtccccgaag ccgccgaaca tgtccgccgt cgcaaccacg acgacatcca
     1321 cgccccgctc gtgtttcaca tgcacgacgt tgcgctcggg cgtgcccgta ttgcgtacgg
     1381 acacaaaccc cggcccgagg attgggtaca tgccctcgag cgcatggatc ccgtagcgtt
     1441 cccacgtttt cggcgtggtg atggtgacat agcgcaaggc gcccaggttg tgggtcgacg
     1501 cccggtaggg ctggtactct ttgcaataac gcatcgagct ggacgacagg atggggcgct
     1561 cctcctcgac gatccagcgg cggaattgag ccaggtccgg ttcgttgtcc acgagcggtt
     1621 tatccacgaa caccggcagc cccgcctcga caaaggggcg gcaccgctcc acgtgctcgt
     1681 gtcccttgtc tgtcgctaca atcacggcgt ccacgtggcc gatgacgtct tcggcccgct
     1741 ccaccacatt gggaatgcgg gacgccgcag caactttggg cgcatccgcc gggtcgtcag
     1801 tccacacatg ggtgaccttc gcccccggga tgctcaggtt cgcctccggc tctttcgaca
     1861 gatagtcggg aatggccggg aacgggcatt ttgccatttc accgggttcg tatccgttaa
     1921 aaatggcgct ccacgaatac ggatgcccgt tgccgtccac catgcccagc atggccagcc
     1981 ggagttcttt gtctccaaat ccgttaaagg ctttcatata tcctcccgca gggcgccggc
     2041 caggtgccgg gctttccgga tgtctcgcga aaacagcgcg gcggaagctc gctgagcgtg
     2101 gaaaatgcca gtggattacg ctgcatgggc acccctgaat tccgccagga cggcgatcaa
     2161 tgcccatacg atagccgatt gccgggaaga agtaaaaagg ccgcgaaatc tgagaatgta
     2221 cacaacgagt gatgcgttcg ccacggtttt cgttcatttc gcccattttg gagcattgct
     2281 tgccgaaaat gtgctactct ttgcctgttc acagtacagt aaggagtacc gcttccatgg
     2341 aaaccctgac cctggcactc ggagaccggg gatatccgat ccacatcggc gagcatgccc
     2401 tgcgccaact gcccgcggaa ctggcccggc tggaggccag gggcgccgtg gccgtctgca
     2461 ccgacgccaa cgtggccagg atccacggcg agagcgtcct ggaatccgtc cgttcggccg
     2521 gggtaacgcc cgtgctgtgt gtgatgcccg ccggagaaga ggccaagcag ctcgcgcgca
     2581 ttgaagaatt ctgcggcatg tttttggagg cggggctgga tcgctccagc gtcgtggtgg
     2641 cgctgggcgg gggcgtgccc ggcgacgtcg ccgggttcgc tgcggcaagc ttcatgcgcg
     2701 ggatccggtt cgttcagatt ccaacgacca tcgtggccca ggtggattcc agcgtggggg
     2761 ggaagaccgg cgtcaaccat gcgctcggaa agaacaccat cggcgcgttt catcagccgt
     2821 ccgcagtcat catcgacctg accttcctga agaccctccc ggaccgggaa cttcgcgcgg
     2881 ggctggccga agtcatcaaa cacggcgtga tcgccgacgc cgccctgttc gaatacctcg
     2941 agtgccacgt cgaacccatc ctggccaaag acctcgacgc actcatgctt cccgtccgtc
     3001 gttcctgtga gatcaaagcc gctgttgtcg ctgaagatga aaaagagcac ggtctccgcg
     3061 ccatcctcaa ctacgggcac accttcgggc acgggatcga ggccgtcacc cgctacggtg
     3121 tctttctcca tggcgaagcc gttgccctcg gcatggaggc cgcggcaagc ctggcgcggc
     3181 agctcggcct gctcgacgag gaagccgccg cccgccagcg cgcctgcctc gaatcctatg
     3241 ggctgccgg
//
LOCUS       DAOPBQ010000436         3233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23737_length_3233_cov_3.729704, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000436 DAOPBQ010000000
VERSION     DAOPBQ010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23737_length_3233_cov_3.729704"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PKW60_12460"
     CDS             complement(<1..767)
                     /locus_tag="PKW60_12460"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPK00291.1"
                     /translation="MNSSVEITGMAHGGHGVGRVEGQVCFVAYALPGDVVRIDVVRRT
                     RGVLWAVITELVQPSPHRIAAPCPVFGRCGGCQWLTFEYPAQAEWKCQIVRDCFQRIG
                     RLAIDPEWADSPDLYLGYRTRAEFHEHGGAAGFYARGSHDVVDIASCPLCHDKLNAAL
                     DRIRAARPGQSVEVTVNPEGDDVLVWTRQPSPSLQEAFPQYDCGREHDTRHGFMFDGV
                     PIVNGAFSQSSLLLNRMLLEVVHTLVGQPARLLDLYCG"
     gene            complement(812..1330)
                     /locus_tag="PKW60_12465"
     CDS             complement(812..1330)
                     /locus_tag="PKW60_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPK00292.1"
                     /translation="MAEKRKDYLSWDDYFMGLAILSAQRSKDPNSQVGACIVDDHNKI
                     VGIGYNGFPSGCSDDELPWAREADSTLDTKYPYVCHAELNAILNTNIADTSGCRLYVT
                     LFPCNECAKLIIQSGIREIIFLSDKYRDSDSNRAARRMFDMVGVRYRIFRAQTPRIVI
                     DFSEQAAKATQV"
     gene            1458..1534
                     /locus_tag="PKW60_12470"
     tRNA            1458..1534
                     /locus_tag="PKW60_12470"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1492..1494,aa:Val,seq:cac)
     gene            complement(1607..>3233)
                     /locus_tag="PKW60_12475"
     CDS             complement(1607..>3233)
                     /locus_tag="PKW60_12475"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPK00293.1"
                     /translation="VLQADLNALGVADFGNPQGGGLELFYQDVPMTVSRWPNDGFVRI
                     QELSVEDGHQIHGVKGSMTGKFVYDGERPRRWLQEEDPWLHGYWFWDWSDERKPIHAI
                     DVENHTIELAEPAHSYGYRKGQWYYAFNMLCELDTPGEWHVNRETGMLYFWPPEPIRS
                     GAAVVSVIPTIISASDVSFVTLRGFTFEACRSTALVMRGGAINRVVACVFRNGGADAV
                     RVTDGTNHGVIGCDIYQMAGGGITLTGGDRITLSPADHFAENNHIHHFARWYRMYHTG
                     IRLNGVGNRAAHNLLHDAPHMAMGFAGNDHCIEFNEIYNVCYESNDAGAIYTGRDWTM
                     RGHLLRYNYLHDITGFENRGCMGMYLDDMFSSADLVGNVFCNVTRAVLIGGGRDCTVT
                     NNIFVDCKPAIHLDARALGWAHGHADGWLQEAAEKGTLSGIAYNEPPYSERYPALAVM
                     LDKEPKAPEDNVFSRNICWGGEWDGMQEEAKKYVQIENNLIQEDPHFVDAANRDFRLK
                     DDSPAFALGFEPIPMEKIGLYESPDRASWPPQR"
BASE COUNT          616 a          976 c          995 g          646 t
ORIGIN      
        1 ccgcaataga gatcaagcaa gcgtgcgggc tgtcccacga gcgtatgaac tacctccagc
       61 agcatgcggt tcagcaaaag gctcgattgt gaaaatgcgc cgttcacgat cggcactccg
      121 tcgaacatga atccgtgccg cgtgtcgtgt tcgcgcccgc aatcgtattg cggaaacgcc
      181 tcctgcagcg acggactcgg ttgacgcgtc cacacgagca catcatcgcc ttcggggttt
      241 acggtaacct cgacggactg cccgggccgc gccgcgcgta tccggtcgag ggcggcgttg
      301 agtttgtcgt ggcacagggg gcaggaggcg atatcaacca cgtcgtggga gccgcgcgcg
      361 taaaaaccgg ctgccccgcc atgttcgtgg aattccgcgc gcgtgcggta gccgaggtac
      421 aagtccggcg agtcggccca ttcgggatcg atcgcgagcc ggccgatgcg ctgaaagcaa
      481 tcgcgcacaa tctggcattt ccactcggcc tgcgcgggat actcgaaggt cagccactgg
      541 cagccgccgc agcgcccgaa cacgggacag ggcgccgcga tccggtgagg cgacggctgc
      601 acgagctcgg taatgacggc ccacaaaacc ccgcgggtcc ggcgcacgac atcgatgcgc
      661 accacgtcgc cgggaagggc ataggcaaca aaacagacct ggccttcaac gcggcccacg
      721 ccatggccgc catgggccat accggtgatt tctacgctgg aattcatgat gttttcggga
      781 tggtctttcg tggttggtag cggccgaggc gttacacctg ggtagctttg gcggcctgtt
      841 cggagaaatc aatgacgatg cgcggcgttt gtgcccggaa tatgcggtaa cgcacgccga
      901 ccatgtcgaa catccgccgt gcggcccggt tgctgtccga atcccggtac ttgtccgaga
      961 gaaaaatgat ctcgcgaatg ccgctctgga tgatcagttt cgcacattcg ttgcagggaa
     1021 acagggtgac gtacaggcgg cagcccgagg tgtccgcgat gttcgtgttc aggatggcgt
     1081 tcaattcggc gtggcacacg tacggatact tggtgtcgag cgtgctgtca gcctcacggg
     1141 cccacggcaa ttcatcgtca ctgcagccgg acggaaatcc attgtaaccg atgccgacga
     1201 tcttgttgtg gtcatccacg atgcacgcgc ctacctgcga attggggtct ttgctgcgct
     1261 gtgcggacaa aatcgccagc cccatgaaat agtcgtccca gctgagataa tccttgcgtt
     1321 tctccgccat ctccggctcc ccttctgccg ccgtccgcac catgtcgcaa cgacggaaag
     1381 ccgagtataa cacagccagc ggagggaccg tttgttgatc gttccggcgg ccatatgcta
     1441 cgatccggtt ctaaacaggg cgactagctc aggtggttag agcgccgtct tcacacggcg
     1501 gaggtcactg gttcgagccc agtgtcgccc accatcagac acccggcgcc ggagtgtacg
     1561 cacccggcgc cggttttttg ttttcggctc tcgttcgctc tgggcgttag cgttgcggcg
     1621 gccaggaggc gcgatcgggg ctttcgtaaa gacctatctt ctccatggga atcggctcga
     1681 accccagcgc aaaggcgggg gaatcgtcct tcagccgaaa atccctgttc gcggcatcca
     1741 caaaatgcgg gtcctcctga atcagattgt tctcgatctg aacgtacttc ttcgcctcct
     1801 cctgcatgcc gtcccattca ccgccccagc agatgttgcg ggaaaagaca ttatcttcgg
     1861 gggccttggg ttctttgtcg agcatgacgg ccagcgcggg gtaccgctca ctgtacggcg
     1921 gctcgttgta cgcgatgccg gagagcgtgc ctttttccgc ggcctcctgg agccagccgt
     1981 cagcatggcc gtgggcccag cccagagcgc gcgcatcgag atgtatggcc ggtttgcagt
     2041 ccacgaagat gttgttggtc accgtgcagt cgcgcccgcc gccaatgagg acggcccgcg
     2101 tgacgttgca gaacacgttg ccgaccaggt cggccgatga aaacatgtcg tcgaggtaca
     2161 tgcccatgca accccggttt tcaaatccgg taatgtcgtg caggtagttg tagcgcagca
     2221 gatggccccg catggtccag tcgcggccgg tgtagatggc gcccgcgtcg ttggattcat
     2281 aacagacgtt gtagatctca ttaaattcga tgcaatggtc attgcctgcg aatcccatgg
     2341 ccatatgggg ggcgtcatgg agcaggttgt gcgccgcgcg attgcccacg ccattgagcc
     2401 ggattcctgt gtgatacatg cggtaccatc gggcaaagtg gtggatgtgg ttgttttccg
     2461 caaaatgatc ggccgggctg agtgtgatgc ggtcgccacc cgtgagcgtg atgcctccac
     2521 cggccatctg atagatatcg catccgatca cgccgtgatt tgtgccgtcg gttacgcgca
     2581 ccgcgtctgc gccgccatta cggaacacgc acgccaccac ccggttgatc gcgccgccgc
     2641 gcatcaccag cgccgtggaa cggcatgcct caaaggtgaa tccgcgcaag gtcacgaagg
     2701 agacatcgct ggcggagata atcgtgggga tcaccgacac aacggcggcg ccgctgcgta
     2761 tgggttcagg cggccagaaa tagagcatgc cggtctcgcg gttaacgtgc cattcgccgg
     2821 gggtatcgag ctcgcagagc atgttgaacg cgtaatacca ctggcctttt cggtagccgt
     2881 aactgtgggc gggctcagcc agctcgatcg tgtggttttc cacatcgatg gcgtgaatgg
     2941 gcttgcgctc atccgaccag tcccagaacc agtagccatg aagccaggga tcctcttcct
     3001 gaagccaacg ccgcggccgc tcgccgtcgt acacgaattt gccggtcatg ctgcctttga
     3061 cgccgtggat ctggtggccg tcttcgaccg aaagctcctg aatgcgcaca aatccgtcgt
     3121 ttggccagcg cgagaccgtc atgggaacgt cctgataaaa cagctcgagg cccccacctt
     3181 ggggattgcc gaaatccgcg acgccgagcg cattcaggtc ggcctggagc acg
//
LOCUS       DAOPBQ010000437         3232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23753_length_3232_cov_4.128738, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000437 DAOPBQ010000000
VERSION     DAOPBQ010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23753_length_3232_cov_4.128738"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..1263)
                     /locus_tag="PKW60_12480"
     CDS             complement(325..1263)
                     /locus_tag="PKW60_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPK00294.1"
                     /translation="MYTGRGWGFTLIELLVVIAIIGILAGFLAVGLPRALELAKIRDV
                     ETDFNGIRNALSVYAAQHGTYPPAYGYFTGYRDDTGAELYYLAPYMARIGEFNNLDLY
                     DRFSTTDDANQNGVVDRLEHTPAGIAAGPGRFLFPDDTTLPYNGSAAPSKNPAPYIYL
                     PVNKRQAAQYATSLWKQWNKQAGAAFVKFMYARTDPGNDPLDAMSFPPAQYDAYALVS
                     VGPWGQTGGITDNSGPGGSIDTYHRDVMRAYFLATRDANENGLLDFDFRARSKLGEGK
                     RDAFVIDGLYHLPNEFQEWADSGFTGSNYGPMIGTP"
     gene            1427..>3232
                     /locus_tag="PKW60_12485"
     CDS             1427..>3232
                     /locus_tag="PKW60_12485"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPK00295.1"
                     /translation="MATLNTEQRRAVTHPGGPALVLAGAGAGKTRVIIERLAWLVDER
                     GVDPRHLLAMTFTNRAAGEMRTRLAERLGMRQVGAWLGTFHSFGLYVLRRDIEKLGRP
                     RHFTVVDDGDQLTLMKRLIKELPARYSRVSPREALQWISVRKQGLDSLNPDAGASHEE
                     ETCQALWKRYHEALVKSNAVDFDDLLVLTARLFQSHDDVRARYGKRFTWVHIDEYQDT
                     NRAQYVIARELVRDHGNLFVVGDEDQSIYAWRGATIRNILDFETDFPGARLYRLEENY
                     RSTRTILSVANAVVSHNTQRLGKTLRTRRDDTSPVRLYEAEDADDEARFVAEDIATGG
                     LSPRDTAVLFRTHGQSRLIEEALRRQGLAYVVIGGVSFFGRKEVKDLLAYLRLAVNPD
                     DDQAVRRVLNVPNRGIGEHSMEIIETYATERDCRLFTVLREIEYDQSLPLRAREGAAR
                     FVHVVDQLAEKATSTPLAGLIGTLLDDIGYRDYVRRSDERDFRTGLEVVEEFVSACVR
                     FDVEQRGTVHTFLEELALASESDQLDPNAPAVKLLTCHSAKGLEFDNVYLVGLEEGFL
                     PHASALDSESEVEEERRLCYVAMTRARNRLTLSLSR"
BASE COUNT          627 a          967 c         1035 g          603 t
ORIGIN      
        1 gtcaccgaag ccatgctggc cgaatatttg tcccgccggc aggccgcctt cgacgaaacc
       61 agcgaaaacc gctaagccgc ctcctcagag atacggcccg cccgcccttc tgtaagcacg
      121 ggccatcaaa tgccaggcgc agcaccagcg tggctcaggc ggtataagct catttgcgga
      181 ttcggcgtga ggcggttagg ccagagacaa ggcaggcagc ggccttctcg cgcgggcgga
      241 gtgcaaccct taatgcagca gtagtctgcc gtctgcagac agaccggacg gcacgggcac
      301 agatggccgg atggtgcgac ggcgtcaggg ggtgccgatc atggggccgt aattcgaacc
      361 ggtaaatcct gagtcggccc attcctgaaa ctcatttggc agatgataga ggccgtctat
      421 gacaaaggcg tcacgttttc cttcgcccag tttactacgg gcccggaagt cgaagtcgag
      481 caggccgttc tcgttggcat cgcgcgtggc gaggaaatag gcgcgcatca catcccgatg
      541 gtacgtgtcg atactgcccc ccgggcccga gttgtcggta atgccaccgg tctgccccca
      601 tggacccacg ctgaccagag cgtacgcgtc gtactgcgca ggaggaaacg acatggcatc
      661 tagaggatcg ttgccgggat cggtcctcgc gtacataaac ttgacgaacg cggcgccggc
      721 ctgcttgttc cattgtttcc agagcgaagt ggcatattgc gcggcctgcc gtttgttgac
      781 gggcaggtag atgtacggag cgggattttt ggacggtgcg gcgctgccat tgtacggcaa
      841 ggtcgtgtcg tctgggaaca ggaagcggcc cggcccggct gcgatacccg ccggcgtatg
      901 ctcaaggcgg tctacgacgc cattctggtt ggcgtcgtcc gtggtcgaga accggtcata
      961 gaggtccagg ttgttgaatt cgccgatgcg ggccatgtag ggcgcgaggt aatagagttc
     1021 agcgccggta tcgtctctat agcctgtaaa atacccgtag gccggcggat aggtcccgtg
     1081 ctgcgctgca tagaccgaca gcgcgttgcg gatgccattg aaatcggttt caacatcccg
     1141 tatcttggcc agttcaagcg cccgcggcag gcccacagcc agaaagccag caagaatccc
     1201 gatgatggcg ataacgacga ggagttcgat gagcgtgaag ccccagcctc tgcccgtata
     1261 catgggtcgc acctccctgt tacaatgcgt agtatgctgc tcaatctcat gataacatgg
     1321 gtcgggctaa gcgtcaaatg atatcctgtc aaatttgccg gttctctcga tgtaccaacg
     1381 gcatagcgaa actacgaaaa ttgtgaactc tatgatgacc gcagatatgg caacccttaa
     1441 taccgagcaa cggagagctg tcacacaccc tggagggccc gcgcttgtgc ttgcgggcgc
     1501 gggcgccggc aagacccggg tcattatcga acggctggcc tggctcgtgg atgaacgcgg
     1561 cgtggacccg cggcatttgc tggccatgac cttcacgaac cgcgccgcgg gagagatgcg
     1621 cacccggctg gccgagcggc tcgggatgcg ccaggttggc gcgtggctgg gcaccttcca
     1681 ttcgtttggc ctgtacgtcc tgcggcggga catcgagaag ctcgggcgtc cacggcactt
     1741 caccgtggtg gacgacggtg atcagctcac cctgatgaag cggctcatca aagagctgcc
     1801 ggcccggtat tcccgtgtgt cgccgcggga ggcgctccag tggatcagcg tgcgaaagca
     1861 gggcctcgat tccctgaacc cggacgcggg ggcatcgcat gaggaagaga cctgccaggc
     1921 cctgtggaag cggtatcatg aggccctcgt caaaagcaac gcggtcgact tcgatgatct
     1981 gcttgtattg accgcgcgcc tgttccaaag ccacgacgac gtgcgcgccc ggtacgggaa
     2041 gcgctttacc tgggtccata tcgacgaata ccaggacacg aaccgggccc agtacgtgat
     2101 cgcgcgggaa ctggtgcgcg accacggtaa cctgttcgtc gtcggcgatg aggatcagag
     2161 catttacgcg tggcgcgggg cgaccatccg gaacatcctg gatttcgaaa ccgatttccc
     2221 cggcgcccgg ctgtatcgtc tcgaagaaaa ctaccgcagc acccgcacca ttctgagcgt
     2281 cgccaacgcc gtggtatctc acaacaccca gcgtctcggc aagaccctgc ggacccgtcg
     2341 cgacgatacc agccccgtgc gcttgtacga ggcggaagac gccgacgatg aggcgcggtt
     2401 tgtggccgaa gacatcgcca cggggggctt gtccccgcgg gacacggcgg tgctctttcg
     2461 cacgcacggc cagtcgcggc tgatcgagga agccctgcgg cgccagggac tggcctacgt
     2521 ggtgatcggc ggggtctcgt tcttcgggcg caaagaggtc aaagatctcc tggcctacct
     2581 tcgtctggcg gtcaatcccg acgatgatca ggccgtgcgg cgggtattga atgtccccaa
     2641 ccgggggatc ggcgagcact ccatggagat catcgagacc tacgccacgg agcgtgattg
     2701 ccgccttttc accgtgttgc gcgagatcga atatgatcag agccttccct tgcgcgcgcg
     2761 ggaaggtgca gcccgcttcg tgcacgtcgt cgaccagctg gccgagaaag ccacaagcac
     2821 ccccctggcg ggcctgatcg ggaccctgct ggacgacatc ggctaccggg actacgtgcg
     2881 gcgttccgac gagcgggatt ttcgcaccgg cctcgaggtc gtggaggaat ttgtctcggc
     2941 ctgtgtccgg tttgatgtag aacagcgcgg cacggtgcac acgtttctcg aagagctggc
     3001 cctggcgagc gaatcggatc agctcgaccc caacgcaccg gctgtgaagc tcctgacctg
     3061 ccacagcgcg aaaggccttg agttcgataa cgtgtacctg gtaggtttgg aggagggttt
     3121 tttgccgcac gccagcgcgc tggactccga gtctgaggta gaggaagaac gccggctgtg
     3181 ctacgtcgca atgactcgcg cccggaaccg gctcacgctg tcgctgtcgc gc
//
LOCUS       DAOPBQ010000438         3230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23772_length_3230_cov_3.668346, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000438 DAOPBQ010000000
VERSION     DAOPBQ010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23772_length_3230_cov_3.668346"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PKW60_12490"
     CDS             complement(<1..348)
                     /locus_tag="PKW60_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HPK00296.1"
                     /translation="MPEGRKTIHVICNTHWDREWAYPFEETRLLLLDFMDSLLDLLDR
                     DSDFHSFLFDSQTIALDDYLELRPERREDMERHVTAGRLVVGPWYSLPEEYIVNGESL
                     VRIPGADHQPPRGN"
     gene            523..1818
                     /locus_tag="PKW60_12495"
     CDS             523..1818
                     /locus_tag="PKW60_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPK00297.1"
                     /translation="MAAVYPNQRPNILVILTSDHQFRAIGAAGNPAIRTPHMDWIARG
                     GVHCTHCHVSNPVSTPSRASFLTGQYGFRNGVTVSRQPMRPGVPQVARELSQRGYQTA
                     FTGKWHNDGRPCHHGFYLMRNVFLGGMHDYDRIPVVQEAGDPPVEIRRHPSEVFTDGA
                     LELLERGCVEPFALFVWYTAPHDPRTPPERCEALYPPEAISLPPNFMPAPPFDPGTLD
                     IRDEKLLPRPLNPQAVQGEIARYYAMLTHLDNQIGRILHRLQEKGILGNTVVILASDN
                     GLSLGAHGLLGKQNMYEEGVRVPLLIQGPGFQSGVQCDALVDLMDVMPTLLDIAGAPI
                     PAAVQGRTLTPLLSGRRTRLRDSIFCHYDDFFRMVRTPRHKLVRHLKTGREELFHIEI
                     DPYEQHNLVESARYAPVLADLRARLNHWRRTLHDPTLKD"
     gene            complement(1848..2696)
                     /locus_tag="PKW60_12500"
     CDS             complement(1848..2696)
                     /locus_tag="PKW60_12500"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPK00298.1"
                     /translation="MFIRVSMTLMMLASAGFAHSEPFSLDVMSFNIRFGTANDGENSW
                     EHRRDLVVTMLRERAPDLVGMQECLDFQADYIVEKLPEYRWFGMGREADATGERMAVF
                     YKWQQFSPIEVKNFWLSETPDVPGSRSWDAACSRMVTAARFYHHQTRQYLTFCNTHLD
                     HVSEAARQKGAEVLLKYIASIDPALPVVVTGDFNSDAVRSAAWTTLVQGDIRDAWLVA
                     GATAGPTVTFNAFKPLQPGDDSRIDWILVRGPIAVERCETVVYGEDGHYASDHFAVTA
                     HLNVTP"
     gene            complement(2767..>3230)
                     /locus_tag="PKW60_12505"
     CDS             complement(2767..>3230)
                     /locus_tag="PKW60_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK00299.1"
                     /translation="MHGFGPGEQRLLVAAALLHDVGWPVRPEAHHKGSRDLILKAPLA
                     GFSEEERRMIACLARYHRKAHPSARHKVYRDLNQSAREKVRMLAAILRIADGLDRAHA
                     ATVKALEVCRDGQTLTIAVQQTPGCGIDLMGAERKKGLFEAVFGVDVVIRG"
BASE COUNT          599 a         1020 c          964 g          647 t
ORIGIN      
        1 gttaccgcgg ggcggctggt ggtcggcccc tggtattcgc acgagggatt ccccgttcac
       61 gatgtactct tccggcagcg aataccaggg gccgaccacc agccgccccg cggtaacgtg
      121 ccgttccata tcctcgcggc gctccggccg gagctcgagg tagtcatcca gcgcgatggt
      181 ctgcgaatcg aacagaaatg aatgaaagtc gctgtcgcga tccagcaaat ccagcagact
      241 gtccataaaa tcgagcagca acagccgtgt ctcttcgaag gggtaggccc attcgcggtc
      301 ccagtgggtg ttgcaaatga cgtggatggt ctttcgtcct tcgggcatga gcgcgttcct
      361 ccttatgcat actgagcgca gcgtgcaagt ttaccatgcg ccggcagcgg gattacactt
      421 ctcattgact gccggcttgc gcgcccgagc tgaaatggcc taccctgtgc ctgtcctgag
      481 gcgtgagggc ggcctgagca cggcgcccgc agggggaggt ctatggcagc ggtgtacccg
      541 aatcaacgtc caaatatcct cgttatcttg accagtgacc atcagttccg cgcgatcggc
      601 gccgcgggca atccggcgat ccgcacaccc cacatggact ggatcgcccg tgggggcgtc
      661 cactgtactc attgtcacgt gtccaacccg gtctcgacac ccagccgggc ctctttcctc
      721 accggccagt atggatttcg caatggcgta acggtttcca ggcagccgat gcgccctggc
      781 gtgccccagg tggcccgcga actgtcccaa cgcggttatc aaacggcctt tacggggaag
      841 tggcataacg acgggcggcc ctgccaccat ggtttttacc tgatgcgcaa cgtctttctc
      901 ggggggatgc acgactacga ccgcatccct gtggtgcagg aagccggtga tccgcccgtc
      961 gagatcaggc gccacccgtc agaagtgttt acagacggcg cgctggaact cctggagagg
     1021 ggatgcgtgg aaccattcgc tttattcgtc tggtacaccg cgccgcacga tccgcgtact
     1081 cctcctgaaa gatgtgaggc cttgtatccg cccgaagcca tctctctgcc gccgaatttc
     1141 atgccggcgc cgccgttcga cccgggcacg ctcgatatcc gcgatgagaa actcctgccg
     1201 cgtccgctca atcctcaagc ggtccaaggg gaaattgcgc gctactatgc catgctgacc
     1261 catttggaca accagatcgg gcgcattctt caccgtttgc aggagaaggg gatcctcggc
     1321 aataccgtgg ttatcctggc cagcgacaac ggactgagcc tgggcgccca cgggctcctg
     1381 ggcaaacaaa acatgtacga ggaaggggtc cgtgtgccct tgctcatcca gggcccgggt
     1441 tttcagagcg gcgtccagtg cgatgcactg gttgatctca tggacgtgat gcccactttg
     1501 ttggacattg ccggcgcgcc aatccccgct gcggtgcagg ggcgcacgct cacgcccttg
     1561 ttaagcgggc gccgcacgcg gcttcgcgac agcattttct gccattacga cgattttttc
     1621 cgtatggtgc gcacgccccg gcacaagctg gtccgccacc tcaagaccgg gcgggaagag
     1681 ctgttccaca tcgagatcga cccgtatgaa cagcacaacc tcgttgaatc cgcgcgctac
     1741 gcgcccgtcc ttgccgatct ccgggcacgg ctcaaccact ggcgcagaac ccttcacgac
     1801 cccacgctca aggactgact tatgcgcgcg ggcgctttgc tgcgggccta aggcgtgacg
     1861 ttcaggtgcg cggtgacggc gaaatggtcg gacgcgtaat ggccatcttc cccgtagacc
     1921 acggtctcgc aacgctcgac tgcgatgggt ccgcgaacga gtatccagtc gatgcggctg
     1981 tcgtcgccgg gttgcagcgg cttgaacgcg ttgaacgtga ctgtcggccc ggcggtcgcg
     2041 cctgcaacca gccaggcatc ccgaatgtcg ccctgcacca gcgtggtcca ggcagcggag
     2101 cggaccgcgt ctgagttgaa atcgcccgtc acgacgacag gcagggccgg gtcaattgac
     2161 gcgatgtatt tcaggagcac ctcggctccc ttttggcggg cagcttcgct cacgtggtct
     2221 aaatgggtgt tgcagaacgt gagatattgg cgcgtctggt gatgatagaa gcgggcggcc
     2281 gtcaccatgc gcgagcacgc ggcatcccag gagcggctgc ccggaacgtc tggcgtttcc
     2341 gagagccaga aatttttgac ctcgatgggg ctgaactgct gccatttata aaatactgcc
     2401 atacgctcac cggtggcgtc ggcttcgcgc cccatgccga accagcggta ttcgggcagc
     2461 ttttcgacga tgtagtctgc ctgaaaatcc aggcattcct gcattccgac caggtcgggg
     2521 gcacgttccc gcaacatcgt gacgaccaga tcacggcggt gctcccacga gttctcgccg
     2581 tcatttgcgg ttccaaaacg gatattaaag gacatgacgt ccaggctgaa gggttccgaa
     2641 tgggcaaagc cggcggaggc cagcatcatc aaggtcatgc ttactcggat gaacacgtgc
     2701 gttctccttt ccacgttgtt ctgatttcat tggacagctt cagtaaagcg gatttgtccg
     2761 gctcgttcaa ccgcgaatga cgacatccac gccgaaaacc gcctcgaaca gccctttctt
     2821 acgttctgca cccatgagat cgatgccgca gcctggcgtc tgttgcactg cgattgtcaa
     2881 cgtctggccg tcgcggcaca cttcaagcgc cttgaccgtt gccgcgtggg cccggtccaa
     2941 accgtccgca atccgcagta tggccgcaag catgcggact ttttcccggg ccgactggtt
     3001 cagatcccgg tacaccttgt gccgggcgct ggggtgcgct ttgcggtgat agcgggccag
     3061 gcaggcaatc atgcggcgct cctcttcact gaatcccgcc agcggtgctt tcaggataag
     3121 gtcgcgcgag cctttatgat gtgcctcggg gcgcacgggc cagcccacgt catgcagcag
     3181 cgccgccgca acaagcagcc gctgttcgcc cggcccaaaa ccgtgcattc
//
LOCUS       DAOPBQ010000439         3228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23793_length_3228_cov_2.822250, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000439 DAOPBQ010000000
VERSION     DAOPBQ010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23793_length_3228_cov_2.822250"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKW60_12510"
     CDS             <1..226
                     /locus_tag="PKW60_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00300.1"
                     /translation="RVRAAPGATVEAPDTNIVFLEVPDASSFVAALKVRRILAGATGP
                     TQIRVVFHLDIGDSALETAIAGFREVAGLF"
     gene            376..>3228
                     /locus_tag="PKW60_12515"
     CDS             376..>3228
                     /locus_tag="PKW60_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPK00301.1"
                     /translation="MKKKAIGILIKLVFTALLFILLFRPQTFGLKQDFFGGVTPAKMW
                     EELRGTHWGSLIPWLLFAVVVKLAGMAAGILRWRLLLKGQGLAIPFKYLVESWFVGRV
                     FGIFLPSTIGLDGYRLYDSSVYTGETVKCATVIAVEKLIGFVALTFLLFLTFPLGFRL
                     LNINGVIFSGILVVLAGFVTVAFILLLNPRVIQILVAVVPTPAKVSNTLNKLGLAATA
                     YSGNRVLLLQATALGLAVHLGTCFMYFGTMMAIRAENTSLWDILFASPVLIYGTVLGP
                     SIGGEGIREIVFVSLLSGTAGAAAAALFAHLGWWVGEFVPFLIGMPILILRSRRPKDD
                     MAAELARMRSKASQTQVLVHLTPGEISAYRRNMTNCLLAGALAGLAGGAITGLAEAAW
                     VIAHHAGYTEWSAYWWGPLVYAGLFLCAGLAVAGGLTLVYLLARRFPSPRFDFALTFG
                     LLLAFAFLVIGRFCYRRDVLGERALSSGANVTILLLALGILLGAAVLALLATRPFRFG
                     RSGAIGAGVGAVALFVLVGVGLSFRAPGRVAPVFAPAEQAHGPNIILVVADALRADYL
                     PMYSNDARTRAPELSAFASDSILFANCFAQASWTKPSFATIFSGRYPESHTATGKISS
                     LPDEITTFAEVFSEHGYYTKGFSNNPNITSDMNFGQGFTDYVDLKPALYFFAQSSASR
                     LTGYDALRKLWNVAANRLGRKMNVTRFYQPAEVVTREALDWLDSPERPNDAPFLLFLH
                     YMDTHDPFMNHDNPGEGYARVQIPHPEPEEHLERMRHAYGTEIEYMDAHIGALLNGLR
                     ERGLYDDAVILFTSDHGEEFYDHQGWWHGQTLYDEVIQVPLLLHLPGGALAGQVNTDF
                     ARHIDLAPTMIDLAGLPAPQEMPGVALVDGGTFANDGTAHVYAENDFEGIVLQAVRTH
                     TEKLIAANENNPRGLQPAEYYDLASDREEAHNLCDDT"
BASE COUNT          552 a         1024 c          925 g          727 t
ORIGIN      
        1 ccgcgtgcgc gccgcccccg gagccaccgt cgaggcgccc gacaccaaca tcgtttttct
       61 cgaagtgccc gacgcgtctt cgtttgtcgc agcgctcaag gtgcgtcgca tccttgcggg
      121 ggctaccggt cccacccaaa ttcgagtggt gttccatctc gacatcgggg acagcgcact
      181 tgagacggcc atcgcgggat ttcgcgaggt tgccgggcta ttttagactg cgattgacaa
      241 ccagccgcgt gggatgtgag atccctggta ttctgcgccc tttcttgccc ctgacttcag
      301 acgcgtgcta gagttggtgt ctgtgtgacg gctgtgctgg tttcatccat tccccaccgg
      361 gcgggcaggg accctatgaa gaaaaaagcc attggcattc taatcaagct tgtattcacc
      421 gcgctattgt tcatactgct ttttcgccca cagaccttcg gcttgaagca ggattttttc
      481 ggcggagtca cccccgccaa gatgtgggaa gagctgcgcg ggacccactg gggcagcctg
      541 attccctggc tgctgtttgc agtcgtcgtg aagctcgcgg gcatggcggc cgggatactg
      601 cggtggcggc ttctgctgaa aggccagggc ctggccatcc ccttcaagta cctcgtggaa
      661 agctggttcg tggggcgcgt cttcggaatt ttccttccca gcaccattgg tctcgacggc
      721 taccggctct atgattcttc cgtgtatacc ggcgagaccg tcaagtgcgc cacggtgatc
      781 gccgtggaga aactaatcgg tttcgtcgcc ctgaccttcc tcctcttcct gacgtttccg
      841 ctgggattcc ggctgctgaa catcaacgga gtcatttttt cgggaatcct tgtggtactc
      901 gcggggttcg tcacggttgc attcatcctg ttgctcaatc cccgcgtgat tcaaatcctc
      961 gtcgcggtcg tccccacccc cgccaaggtc tccaatacgc tcaataaact cgggctggcc
     1021 gcgaccgcct acagcggaaa ccgggtactc cttctgcagg ccacggccct gggactggcc
     1081 gtccatttgg ggacctgctt catgtatttc ggcacgatga tggccattcg agctgaaaat
     1141 accagcttgt gggacattct cttcgccagc ccggtcctga tctacggcac cgtgctcggg
     1201 ccttccatcg gaggcgaggg gatccgcgaa atcgtctttg tgagtctgct cagcggcacc
     1261 gcgggagctg ccgcggccgc cctgtttgct catctgggct ggtgggtggg cgaattcgtc
     1321 ccgttcctaa tcggcatgcc catcctcatt ctgcgttcgc gccggcccaa ggatgacatg
     1381 gccgccgagc tggcgcgtat gcgcagcaag gcgtctcaga ctcaggtcct cgtccacctg
     1441 accccgggcg agatctctgc gtaccgccgc aatatgacca attgccttct cgcgggcgcc
     1501 ctggcggggc ttgccggcgg tgcgatcacc ggtctggccg aagcagcgtg ggtcattgcg
     1561 catcacgccg gctacacaga atggtcggcc tattggtggg ggccactggt atatgccgga
     1621 ctctttcttt gcgcgggcct ggcggttgcg ggaggcctca cgctcgtcta tctgctcgcg
     1681 agacgctttc cttcgcccag attcgatttc gcgctcacgt tcggcctcct gctcgctttt
     1741 gccttcctcg tgattggacg gttctgttac cggcgcgatg tgctgggcga gcgtgcgtta
     1801 agttctggcg cgaatgtcac aatcctgctc ctggccctcg gcattctgct tggtgcggcc
     1861 gtgctggccc tcctggcaac acggccgttc cggttcgggc gatctggcgc aataggcgca
     1921 ggcgtgggcg ccgttgcctt gtttgtgctg gtgggtgtgg ggctctcctt ccgggcgcct
     1981 ggacgcgttg cccccgtatt tgctcccgcg gaacaggccc atggccccaa catcatcctc
     2041 gtggttgccg acgccctccg ggcggattat ctgcccatgt attcgaacga tgcacgaaca
     2101 cgggcgcccg agctgagcgc ctttgccagc gactccattc tatttgctaa ctgttttgca
     2161 caggcctcgt ggacgaaacc ctccttcgca accatttttt ccggacgcta tcccgaatcc
     2221 cataccgcta cgggcaagat ctcgagtctg cccgacgaga tcaccacatt tgcggaggtt
     2281 ttttcagaac acggctatta caccaaaggg ttttcgaaca atcccaacat cacctcagac
     2341 atgaatttcg gtcagggatt taccgactat gtcgatctca aacccgcgct ctactttttt
     2401 gcccagtcgt cggcatcccg gctcacgggc tatgatgccc ttcgcaaact atggaacgtg
     2461 gctgccaacc ggcttggccg caagatgaac gtgacccggt tttatcaacc cgccgaggtc
     2521 gtgacccggg aggccctcga ctggctggac agccccgaac gcccgaacga tgcgcccttc
     2581 ctgctgtttc tgcactacat ggacacgcac gacccgttta tgaaccacga caaccccggc
     2641 gaaggctatg cccgtgttca gatcccccat ccagagcctg aagagcacct cgaacgaatg
     2701 cgccacgcct atggcaccga aattgagtac atggatgctc atatcggcgc gcttctgaac
     2761 ggcctgcggg agcgggggct ttacgatgac gcagtaatcc tgtttacgtc tgatcacggc
     2821 gaagagtttt acgatcacca gggctggtgg catggccaga ccctctacga cgaagtgatc
     2881 caggtgccgc tgctgctgca tctgccgggc ggagcactgg cggggcaggt caacaccgac
     2941 ttcgctcgcc acatcgatct ggcccccacc atgatcgatc tggccgggct ccccgcgcct
     3001 caggaaatgc ccggggtggc cctggtcgac ggcggtactt tcgccaacga cggcacggca
     3061 catgtctatg ccgaaaacga tttcgaaggg atcgttttgc aggccgtgcg gacccatacc
     3121 gaaaaactca ttgccgccaa tgaaaacaat ccccgcggtt tgcagcctgc cgaatactac
     3181 gatttggcct ccgaccggga ggaagcgcac aatctctgcg acgacacg
//
LOCUS       DAOPBQ010000440         3225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23814_length_3225_cov_3.592744, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000440 DAOPBQ010000000
VERSION     DAOPBQ010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23814_length_3225_cov_3.592744"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PKW60_12520"
     CDS             <1..874
                     /locus_tag="PKW60_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00302.1"
                     /translation="QMGLVFKMYANESQGEKWPSMVKAFPNVMLPTCRIDNPAAVYNW
                     INRFFNGPSVYPEYLTDDNVLICPSDGDGWTRVESDGWRKPNVMLPNGGIDPCRFWEI
                     SYEYYGWALTADNVVQPGRDLNRWPFPQTDFDAGFMVAAECLFSGRPSAQPGGSMFPN
                     NGISVSARNNWENNRRGNAFEGDVTYTSYPTMSEKTMYRLREGIERFMITDINNPSGS
                     AKAQSEIPVMWDLAWTTSDPVNGWSYFNHIPGGSNVLYMDGHVQFVRFPGEWPVCKAY
                     VQMMNLLAVLMGLP"
     gene            1249..>3225
                     /locus_tag="PKW60_12525"
     CDS             1249..>3225
                     /locus_tag="PKW60_12525"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPK00303.1"
                     /translation="MHQIASLLLILTAQTSGETVLAQDGSTAWVIVVAEDALAPEKTA
                     AAELQEHLQAATGAVFPVQHSAASEAKAIVVGPSPLLAEAYPDLDLGALGRDGIVMKS
                     SPARIYLAGGRPRGTLYAVYTFLEEVVGCRWWSSTERYVPEKPALTVPALDTVHVPQL
                     IYREAFYRDAFDGVYAARNKCNGHFEKVPEEYGGHYNILGWCHTFFQLLPPETYFAEH
                     PDWYSEIHGERRAERTQLCLTNEAMRAEFIKKALEWIRRNPEAGLISISQNDWHGYCQ
                     CANCRALDEAAGSPSGSLLHFVNAVAAEIEKEFPDFLISTLAYQYTRKAPVGVTPRHN
                     VIIRLCSIECSFSQPLAIGDQNIEFKRDMEAWSAIAPRLFVWDYVTNFRDYIMPHPNL
                     RVLAPNIRFFVANKTAGLFEQGDSGCSISDFPELRAWLLAHLMWEPERDADALIDEFL
                     HGYYGPAAEPLRAYIDLIHDAVERSGVYLRCYMSDTKEWLPVEDAIRAMQLFDEAEAR
                     AKEDPVVATRVARARMPLDNVVLNRHREIARAAKQAGAAVEVPADPTAFAEQFIARAE
                     GFAANSYREGRDFAEYARMLRLRFRPAGPLPAACVGLSEDDYVVIEDNLFNLHGVKSG
                     WSQLVDDAAAADKAAARMPANHTQWAVQYELRDEV"
BASE COUNT          649 a          913 c         1022 g          641 t
ORIGIN      
        1 acaaatggga ttggtcttca agatgtacgc caatgaaagc cagggcgaaa aatggccgag
       61 catggtcaag gctttcccga acgtcatgct gccgacgtgt cgtatagaca atcctgctgc
      121 cgtgtacaac tggatcaacc gcttctttaa cggcccgtcg gtgtatccgg aatatctgac
      181 agacgataac gtcctcattt gcccgtctga tggggacggc tggacccgtg tggaatccga
      241 cggctggcgg aagcccaatg tcatgctccc caacggcggt atcgacccct gccgtttctg
      301 ggaaatttcg tatgagtact acggctgggc tctcaccgcc gataatgtgg tccagccagg
      361 gagggatctg aaccggtggc cgttccccca gactgatttc gacgccgggt tcatggtcgc
      421 tgcggaatgt ctgttctctg gccgaccttc cgcgcagcca ggcggcagca tgttcccgaa
      481 caatgggatc tcagtttctg ccagaaacaa ctgggaaaat aacagacgtg gcaatgcttt
      541 cgaaggggac gtcacctaca cgagttatcc gacgatgtca gagaagacaa tgtatcgtct
      601 ccgcgaaggc atcgagcggt tcatgatcac cgacatcaac aacccgtccg gcagcgcgaa
      661 ggcgcaatcg gaaatcccgg taatgtggga cttggcctgg acgacgtcgg acccagtaaa
      721 cggctggagc tatttcaacc acatcccggg cggcagcaac gtgctttaca tggacggaca
      781 cgtccagttc gttcgcttcc cgggcgaatg gccggtctgc aaggcgtatg tgcaaatgat
      841 gaacctgctg gcggtgctta tggggttacc gtgatgtgac gcgcggcttc gagaccgggc
      901 tcgccgtggc ctgtctcgcg caagccgaag aggaatgctg aaaacgtcta agggcgcgcg
      961 ggcatcgtac cgcacgccct ttgtcatgcc cgggcaaccc gggtccatcc aaattacgca
     1021 gcgcgtagcg caggcatctt gcctgcgttc ttctcttatg taggggcagg cccctgtgcc
     1081 tgccctggat cagggcggat gcatgggggc tatccggatg ggccgccgcc ggattaagac
     1141 aggcgggatt ctctcgcaac ctgttccaat tcgtggcacg cgggacgttc gacgcgtata
     1201 ctctcggggg aacatgaatt ccaggcacga cccacaggga gagactctat gcaccagata
     1261 gcatcgctcc tcttgattct gacggcgcag acgtccggcg agaccgtatt ggcacaggat
     1321 ggttctaccg cctgggtgat tgtcgtcgcc gaagatgccc tcgcccccga aaaaaccgcg
     1381 gccgcggaac tgcaggaaca tctccaggcg gcgacgggcg cggtgtttcc agtacagcat
     1441 tcagcagcat cggaagcgaa ggccatcgtc gtggggccat cgccgctgct ggcggaagcg
     1501 tatccggatc tcgacctggg cgcgctgggc cgggacggga tcgtgatgaa atcgagtccg
     1561 gcgcgcatct atctggcggg cgggcggcca cgggggaccc tgtatgcagt ctataccttt
     1621 ctggaagagg tagtgggttg ccggtggtgg tcatcgaccg agcgctatgt gcccgagaag
     1681 cctgcgctca cggtgccggc gctggacacg gtccatgtgc cgcaactgat ctaccgcgaa
     1741 gcgttttacc gggacgcgtt tgacggggtt tacgccgctc gaaataaatg caacgggcat
     1801 tttgagaaag ttccggaaga atacggcgga cactacaata ttctgggctg gtgccacacg
     1861 ttttttcagc tgctgccccc ggagacgtat tttgctgaac atcccgactg gtacagcgag
     1921 atccacggcg agcggcgcgc agaacgcacc caattgtgcc tgacgaacga agcaatgcgc
     1981 gctgagttca tcaagaaagc gctggagtgg attcgccgga atcctgaggc ggggctgatc
     2041 tcgatctccc agaatgactg gcacggctac tgccagtgcg cgaactgccg tgccctggat
     2101 gaagcggcag ggtcgccctc gggttccctg ctgcactttg tcaatgcggt ggccgcggag
     2161 attgagaagg agtttccgga cttcctcatc tcgacactcg cctaccagta cacacgaaaa
     2221 gcgcctgtgg gcgtgacgcc gcggcacaac gtgatcatcc ggctgtgctc gatcgagtgt
     2281 tcgttttctc agccgcttgc gatcggggac cagaacatcg aattcaaacg ggacatggag
     2341 gcctggagcg caattgcgcc gcgccttttt gtgtgggatt acgtgacaaa tttccgcgac
     2401 tacatcatgc cgcatccgaa cctgcgcgtg ctggcgccga acatccggtt ttttgtggcg
     2461 aacaaaacgg cgggcctgtt tgaacagggg gattcgggtt gctcgatcag tgatttccct
     2521 gaattgcggg cctggctcct ggcgcatctg atgtgggagc cggagcggga cgccgacgcg
     2581 ctgatcgatg aattcctgca cggatactac ggtcccgccg cggagccgtt gcgcgcatat
     2641 atcgacctca tccacgatgc cgtcgagcgc tccggcgtat atctgcggtg ttacatgagc
     2701 gatacaaagg aatggctgcc ggtagaggac gcgatccgag ccatgcagct gttcgatgag
     2761 gcggaagcgc gcgccaaaga agatcccgtg gttgcgacac gggtggcgcg ggcgcgcatg
     2821 ccgctggata acgtggtttt gaaccggcat cgtgagatcg cgcgcgcggc gaaacaggcc
     2881 ggggcggcgg tcgaagttcc agccgacccc acggctttcg ccgaacagtt tattgcccgg
     2941 gcagaaggtt tcgcagccaa cagctatcgg gaaggccgcg attttgctga atacgcgcgg
     3001 atgctccgcc tgcgtttccg tccggcaggg ccgcttccgg cggcctgcgt gggcctttcc
     3061 gaagatgatt atgtcgtgat tgaagacaac ctgttcaatc tgcatggagt caagagcgga
     3121 tggagccagc tcgtggacga tgccgctgca gcagataagg ccgcggcgcg catgccggca
     3181 aaccatacgc agtgggcggt acagtatgag ctccgggacg aggtg
//
LOCUS       DAOPBQ010000441         3211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23943_length_3211_cov_3.263942, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000441 DAOPBQ010000000
VERSION     DAOPBQ010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23943_length_3211_cov_3.263942"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="PKW60_12530"
     CDS             complement(<1..1746)
                     /locus_tag="PKW60_12530"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPK00304.1"
                     /translation="MDWRDPVEARKHPQQLGQLCFSNPDVAKVIADELRRRIRENPKP
                     GIWSVSQMDWGNYCECGACRSLDEREGTHAASLLTCVNRVARALAAEFPEHRIETLAH
                     EWSRKPPRQLAPERNVIIRLSTSECDFSRPLANPKSLENRRFQDDIDAWARLTSNLYI
                     WDYAANLHNFQAPHPNFDVIQPNLQFFKERGAIGVFEQGCPGPGGEFAALRQYLLGTC
                     LWEPGRGARAVISEFINLYYGPAAQHIRDYLALMTQAMQDAEIPLTCFDGGAWMDYKF
                     VSKAQEIFRKATEAVGDAEPFKQRLELANVPLQYASLVCPPDLLYERYAVTLRRPLGP
                     TLDEYVAVLQGLGITHTVDKEPFEAVVEQIGGVTPPRRIESELRILENEYYLIWVAPA
                     LDGAVIRWRDKKLGVELLQGYRCYGRGPGTLQEWYHTPEAQERAVASGYTVDSPTLYA
                     LALNTQLPNGLAMKREMELRPASPEFAITLTLTNTSQEPISPNVKIHPEFALGNESHP
                     EIWFEENNRWTAVPSAALNPQSMGGDYLKPLNGSRWAFRLPSRGLTLINQFVPYQVES
                     LFYFYNTLDSAEHINL"
     gene            complement(1780..2481)
                     /locus_tag="PKW60_12535"
     CDS             complement(1780..2481)
                     /locus_tag="PKW60_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00305.1"
                     /translation="MHRLIFRYQAGVLLGWLVLVLPAWSQPVWVASDLYDQVGVVVAK
                     SAAPEQKRAAEVFVKYWNLTTGHDASVSHAPEKRVNIWIGRNAIPEELLVDLKFDGLG
                     VDGFILDTLRQPVGNKGEDALKTDRHMLVLGGERGIVYAMYHFFEECMGVRWLAPGEV
                     YVPQHAPPALPEISTRVVPAFEYRYTDYMEGIDDPEFAEAHKLLTDCGFGLFSHTVYE
                     LVPPETYFAEHPEYY"
     gene            complement(2754..>3211)
                     /locus_tag="PKW60_12540"
     CDS             complement(2754..>3211)
                     /locus_tag="PKW60_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPK00306.1"
                     /translation="GRAQVPLMGCYGIGVGRLMSSVMEVRRDEYGPKWPITIAPWQVH
                     LNALKLDQPAVREAAERLYQELCAAGIEVLYDDRNARPGVQFADADLLGIPFRFIASE
                     RNLKDGKLEWKRRDTGESGTIPLEHAAETCAQWVRDAFAEIEAAVPESL"
BASE COUNT          656 a          959 c          938 g          658 t
ORIGIN      
        1 gagattgatg tgttccgcgg agtcgagcgt gttgtagaaa taaaacaggc tctcgacctg
       61 gtacgggaca aattgattaa tgagcgtgag gccccggctg ggaaggcgga aggcccagcg
      121 gctgccattc aaaggcttca ggtaatctcc gcccatggat tgcgggttga gagcggcgct
      181 ggggacagct gtccatcggt tgttttcttc gaaccagatc tcgggatgtg attcgttgcc
      241 cagagcgaac tcggggtgaa tcttcacgtt gggcgaaatc ggttcctggc tcgtgttcgt
      301 caacgtaagt gtaatggcga attccggcga ggccggacgc agctccattt cgcgtttcat
      361 ggcaagacca ttgggaagct gggtgttgag cgccagggca tataaagtcg gcgaatccac
      421 cgtatagccg gacgcaacag cacgctcctg agcctcaggg gtgtgatacc actcctggag
      481 tgtcccgggt ccgcgcccgt agcagcgata gccctggagg agctcaacgc ccagtttctt
      541 gtcgcgccag cgaatgacag ctccatccag cgccggcgcc acccagataa ggtaatactc
      601 gttctccagg atgcggagct ctgactcgat ccgccggggt ggcgtgaccc ctccgatctg
      661 ctccaccacg gcttcaaatg gctctttgtc aaccgtatgg gtaatcccga ggccctggag
      721 caccgccaca tattcgtcga gcgtcgggcc cagagggcgg cgcagagtga ctgcatagcg
      781 ctcgtacaat aaatctggcg ggcagaccag cgatgcatat tgaagcggca cgttcgcgag
      841 ctccaggcgt tgcttaaacg gttccgcatc gcccacggct tcggtggcct ttctgaagat
      901 ctcctgcgcc ttgctgacga acttgtagtc catccaggcg cctccatcaa agcacgtcag
      961 agggatctcg gcgtcctgca tggcctgggt cataagagcc agataatccc ggatatgctg
     1021 cgccgccggg ccatagtaga ggttgatgaa ttccgaaatc accgcgcgcg ccccacggcc
     1081 gggctcccac aggcaggtgc ccaggaggta ttgccgaagc gcggcgaatt cgccccccgg
     1141 ccccggacag ccttgctcaa aaacaccaat cgcgccacgt tccttgaaaa actgcagatt
     1201 cggctgaatg acatcaaaat tggggtgcgg ggcctggaaa ttatggagat tggcggcgta
     1261 atcccagatg tacaggttgg aggtaagtcg cgcccaggcg tcgatatcgt cctgaaaccg
     1321 gcggttttca agagatttcg ggttggccag aggccgtgaa aaatcgcatt cgctggtgga
     1381 aagccggatg atcacgttgc gttccggcgc caattgacgc ggcggttttc tcgaccattc
     1441 gtgtgcaagc gtctcgatac gatgctccgg gaattccgcg gccagcgcac gggcaacgcg
     1501 gttgacgcag gtcagcagcg atgccgcgtg ggtcccctca cgctcgtcga gcgaccggca
     1561 tgccccgcat tcgcagtaat tcccccaatc catctgcgag acgctccaga tgcccggttt
     1621 ggggttctcg cggatgcggc ggcgtaactc atcggcgatc actttcgcga cgtcaggatt
     1681 tgaaaagcag agttgcccca gctgttgcgg atgtttgcgc gcctctaccg ggtcgcgcca
     1741 gtcgatagcc gtcggggcgc gcctcgagcc gtggacgagc taatagtact cgggatgttc
     1801 cgcgaagtag gtctccgggg gcacgagctc atacactgtg tgagaaaaca ggccgaatcc
     1861 acagtccgtc agcaacttat gcgcttcggc aaattccggg tcgtcgatgc cttccatgta
     1921 atcggtgtat cggtattcga atgcaggcac gacacgcgtg ctgatctcag gcaaggcggg
     1981 cggcgcatgc tgcggaacat agacttcccc cggcgcgagc cacctcactc ccatacattc
     2041 ttcgaaaaag tggtacatgg cgtagacaat gccccgttct ccgccaagaa ccagcatatg
     2101 gcgatccgtt ttaagcgcgt cctcgccttt gtttccaaca ggttgacgaa gagtgtccag
     2161 gatgaaaccg tccacaccga gcccgtcgaa tttgaggtca accaggagtt cttccggtat
     2221 ggcatttcgg ccaatccaga tattcacgcg tttttcgggc gcatgggaga cactggcgtc
     2281 atggccggtt gtcagattcc aatattttac aaagacttcc gcagcccgtt tctgttccgg
     2341 cgcagcggat ttggccacca ccactccgac ttggtcgtag aggtccgaag ctacccagac
     2401 cggctgcgac caggcaggca gaaccaacac aagccagccg agcagcacgc ccgcttgata
     2461 gcgaaaaatc agacgatgca ctctccaaaa actcctcttt cgcaatggca tagactcttt
     2521 tgactgtacc ctttgatcct cagactttca accgggttgc ataattgcgg cgcctggcca
     2581 gaggttgttc aggggtaccg tctctgccgg ggaaagcgcc gctcagccga gcggcgtgct
     2641 gaaccagggc cgcatatcca gacaattcgc cactatcggc gatgcggccg gcatgaggcg
     2701 ggcacaattt aaagcccagc cagtctcgtc aggcactgag ccgggccgca ggattagagt
     2761 gattcgggca cagcggcctc gatttccgcg aatgcatcgc gaacccattg cgcgcaggtc
     2821 tcggcggcat gttccagcgg aatcgtgccg ctttctcccg tatcgcggcg tttccactcc
     2881 agcttgccgt ctttcaggtt tcgttcgctt gcaatgaaac ggaatggaat ccccagcagg
     2941 tcggcgtcgg cgaactgcac gcccggccgc gcattccgat cgtcgtacaa cacctcaatg
     3001 cccgcggcac acagttcctg gtagagcctt tcagcggctt cccgcaccgc gggctggtcc
     3061 agtttcagcg cattcagatg cacctgccat ggcgcgatgg tgatcggcca tttcgggcca
     3121 tattcatcac gccggacttc catgacggag ctcatcagac gtccgacacc aatgccataa
     3181 cagcccatga gcggcacctg ggccctgccc t
//
LOCUS       DAOPBQ010000442         3209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23958_length_3209_cov_3.219404, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000442 DAOPBQ010000000
VERSION     DAOPBQ010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23958_length_3209_cov_3.219404"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PKW60_12545"
     CDS             <1..300
                     /locus_tag="PKW60_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00307.1"
                     /translation="EALALELGRSELKAMKVLAPLEKDMGPTLRAAHHDIVRMACALR
                     SGRRHRANEALAYHVLDVMHAFGDSSQSGAHVQIESSCERPAPMPMNPRAGGIDV"
     gene            complement(389..907)
                     /locus_tag="PKW60_12550"
     CDS             complement(389..907)
                     /locus_tag="PKW60_12550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK00308.1"
                     /translation="MDRHIEVVAERELDAQDRELLGQWSRALFGSAEDMYDWATPDWR
                     VLVRVGGQLAAHAAISQRTVSANEEAARVGGIGGVMVSQEFQGKGHAKAAMLRAQEFI
                     RDTLQLEFGFLFCSPRLVPYYQRLGWFPIEGPVSFAQGGSDVTWHEEAMVLPLTNRHW
                     PGPPVDLNGPPW"
     gene            complement(1033..1920)
                     /locus_tag="PKW60_12555"
     CDS             complement(1033..1920)
                     /locus_tag="PKW60_12555"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPK00309.1"
                     /translation="MQNQPITRRDFVKHTALTAGAVGALAAVRSADAQDFPKLPIAVI
                     IGMLPKELSDKEKFELAKRCGIDGVEAYPVEDHAAARAQAEAAREAGIVIHSLLYGGW
                     SALFSDPDEAVVAKGLNEFENALRCAKAMDVSSVLLVPARVSAQVRYAEAYERSQKNI
                     RKILPLAEELGVVIAVENVWNDFLLSPIEFARYVDEFESPCLKAYFDCGNVVKFGYPQ
                     DWIRTLGPRIEKIHLKDYLRKENAFKPLREGEVDWPEVRKALAEVGYTGFCTAEVDGG
                     DEAYLTDLAQRMTLIVTGQ"
     gene            complement(2187..>3209)
                     /locus_tag="PKW60_12560"
     CDS             complement(2187..>3209)
                     /locus_tag="PKW60_12560"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPK00310.1"
                     /translation="QLATEDVRSEQHKTQPLQRLRDTVFQADVQWPSAPDRPFEKDAS
                     LPLEAMTLDADPDTRVQGLLGLADAQSPNTISRLAAALDDPDLQVRETALEKLAILDP
                     GRLVQELVLRLDSPDGAYSAFLEEALPALKQRLERPLLALLRDGETAYTTRIVAVRVL
                     GLMGSERAVHELSSLAFQPGDTNLALAATQALALVASPDALPALKGLTLHPSPGIRTE
                     ALHGLARIGGPIALSAIEAMALNPRENNARIRREAIAYIGLMGADPSIDVLISVMNRY
                     PQTREEVVAALRRLTGLNLGNSPLEWTQWRYKQQKAREEAQEEAPPSVGILGIIPGAS
                     GQQPPQ"
BASE COUNT          605 a          969 c         1007 g          628 t
ORIGIN      
        1 gaggccctgg cattggagct tggccgctct gagctgaagg cgatgaaggt cctggccccg
       61 ctggagaagg atatggggcc cacgctgagg gcggcccatc acgacatcgt gcgcatggcc
      121 tgcgctctcc ggtcagggcg caggcaccgg gcaaacgagg cgctggccta tcatgtgctc
      181 gatgtgatgc acgcgtttgg ggattccagc cagtccggcg cgcatgtcca gatcgaaagc
      241 agctgcgaac ggccggcgcc gatgccgatg aatccgcgcg ctggcggtat cgacgtatag
      301 cacccgaggc aatgaaatga cgttagtgcc cgggagaaaa ctcgggtgtg agcagcgtcc
      361 ccggcaagtc agcgtacagg tcaagcggct accacggagg gccgttgagg tcgaccggag
      421 gcccgggcca atggcgattc gtgagtggaa gcaccatcgc ttcctcgtgc caggtcacat
      481 cggagccgcc ttgtgcaaag ctcaccggcc cttcgatggg gaaccagccc agccgctggt
      541 aatacggcac caggcgcggc gagcagaaga ggaacccgaa ctcgagctgg agagtgtcgc
      601 ggataaattc ctgggctcgc agcatggctg ctttggcatg gcctttaccc tggaactctt
      661 gggataccat gaccccgccg atgccgccga cacgggcagc ttcttcattg gcgctgacgg
      721 tacgttggct gatggccgcg tgggccgcaa gctggccgcc cacgcgaacc agtacccgcc
      781 aatcgggcgt cgcccagtcg tacatgtctt ccgcacttcc gaagagcgcc cgggaccatt
      841 gcccaagcaa ctcgcggtct tgcgcgtcaa gttcccgctc tgcgacgact tcgatgtggc
      901 gatccatggc gtacctcgat gcgtcgttct ttcgtcgcgc gaagagtatc agcacaccgc
      961 gttacgggtc aaaagaccac aggcgccctg gcacagggaa ttgcgggcgc cgtttcggca
     1021 agcgctgtgc gactattgcc cggtcacgat caaggtcatg cgctgggcca ggtccgtcag
     1081 atacgcttca tcgccgccat cgacctcggc cgtgcagaag ccggtatagc ccacttccgc
     1141 gagggccttg cgaacttccg gccagtcgac ttcaccttcg cgcaacggtt taaacgcatt
     1201 ttccttgcgc aaatagtcct tgagatggat cttctcaatg cgtgggccga gggtgcggat
     1261 ccagtcctga ggatagccga atttgactac gttgccgcaa tcgaaatacg ccttgaggca
     1321 ggggctttcg aactcgtcga cataccgggc aaattcgatt gggctcaaca ggaaatcgtt
     1381 ccagacgttc tcgaccgcaa tgaccacgcc cagttcttcg gcgaggggca ggatcttgcg
     1441 gatgttcttc tgggatcgct cgtatgcttc agcatagcgc acctgggcgg agactctcgc
     1501 cggcacaagc agcacgctgc tgacgtccat cgcctttgcg caccgcaggg cgttctcgaa
     1561 ctcgttcagc cctttggcca cgaccgcctc gtccgggtca gagaacaagg cgctccagcc
     1621 cccgtagagc aggctgtgaa tcacaatgcc ggcctcccgg gcggcctccg cctgggctct
     1681 ggccgcggca tggtcttcga cgggatacgc ctcaacaccg tcgatgccgc aacgcttggc
     1741 gagttcgaat ttctccttat cggaaagctc cttgggcagc attccaatga tgacggcgat
     1801 gggaagttta gggaagtcct gggcgtcagc ggatcgaacc gccgcaagcg caccaacagc
     1861 gcccgcggtg agggcagtgt gttttacgaa atcccttcgc gtgattggct ggttctgcat
     1921 gggtcgagtg ctccttttac gtcatcggcg ggaacataca tcgtttttta cccgtcgtat
     1981 cgctttcctg cacggccagc atgagcgggg caggaagttg tgtcaagtct aagcgtatcg
     2041 atggaatcac gccggatcaa gtttagaaaa aatacagctt ccaacgtggg ccgcagcccg
     2101 gattcccaat tcccaatcga gcacagatat tacggactga gaacccggtg cagtccagtg
     2161 cgcggtcttc cagcccggaa ggcagctcat tgcgggggct gttggccgct ggctccagga
     2221 atgatcccga gtataccgac cgagggcggc gcctcctctt gcgcttcttc acgtgctttc
     2281 tgttgtttgt agcgccattg ggtccattcc agagggctgt tacccaggtt gaggccggtc
     2341 agccgccgca gcgcggccac cacctcctcc cgggtctgag gatagcggtt catgacgctg
     2401 atcaggacat cgatggaagg atccgcgccc attaaaccga tatatgcgat ggcttcccgc
     2461 cggatgcgcg cattattttc gcgtgggttc aacgccatcg cctcgatggc ggagagcgcg
     2521 atggggcctc caattcgcgc caggccatgc agggcctccg tgcggatacc ggggctggga
     2581 tgaagcgtca ggcctttcag tgccggcagg gcatccgggg aggcgaccag agccagggct
     2641 tgggtcgcag ccaaggccag gttcgtgtcg ccgggttgaa aagccaggga tgacagctcg
     2701 tgaaccgctc gttcactgcc catcagcccc aatacacgca cggccacgat tcgcgtggta
     2761 tacgcggtct caccatctcg cagaagggcc agcaagggcc gctctaagcg ctgtttgagc
     2821 gcgggcaacg cctcttccaa aaaggcggaa taggcgccat ccggggaatc caggcgcaga
     2881 accagctcct gtaccaggcg gccagggtcg aggatggcaa gtttctcgag cgcggtttcc
     2941 cggacttgaa ggtccggatc atccagcgcg gctgcgagcc ttgatatcgt gttcggtgat
     3001 tgggcgtcgg cgaggccgag caggccctgc acgcgcgtat cagggtcagc atccagcgtc
     3061 atggcctcaa gaggcagcga tgcgtctttt tcaaatggcc ggtccggcgc actgggccat
     3121 tggacgtccg cctggaatac agtgtcccgt aaccgttgga gtggctgcgt tttgtgttgc
     3181 tcggaacgga catcttccgt tgcgagctg
//
LOCUS       DAOPBQ010000443         3208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23964_length_3208_cov_3.214082, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000443 DAOPBQ010000000
VERSION     DAOPBQ010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23964_length_3208_cov_3.214082"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PKW60_12565"
     CDS             complement(<1..636)
                     /locus_tag="PKW60_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPK00311.1"
                     /translation="MNVAQIIDRLRSDSDFTRHLTAWRTVPAQPARYAGFPEALNPKL
                     VQGLQRRGIQNLYTHQQEAVEAALAGENVCVVTPTASGKTLCYNVPVLNTLMQEHEAR
                     ALYIFPTKALSQDQVHELQDTIEDLEVKIGTYTFDGDTPGSARKAIRQAGHIVVTNPD
                     MLHTGILPHHTKWLRLFENLRYVVIDELHHYRGVFGSHLANVLRRLKRICSF"
     gene            complement(1264..2772)
                     /locus_tag="PKW60_12570"
     CDS             complement(1264..2772)
                     /locus_tag="PKW60_12570"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPK00312.1"
                     /translation="MNYRLVTKYLGHFSFAIGLLMLPSALWAVLYREWGGLTALLGGT
                     GVSFLTGYLLTLAGRNAPAEFHQREGLGLVGLSWILLAGLGGLPYLFGGVFSNPVDAY
                     FECMSGFTTTGSTVMTAACFATTPKCIMFWRSFTHWLGGMGIVVLFIAVLPYLGAGGK
                     QLFKSESPGPDPRGLKPRIKDTASILYKIYFGFTIAQTAALMLCGMSLFDALCHTFAT
                     LGTGGFSTQAASIGAYDSVAIEMVLTFFMICAGSNFALHFALFTGNWRALLRDTEWLV
                     YLAILGACTLAITVNLMGAEGSFPVGDSPLPPQEKVFFPSAGEALRHASFNVASVMTT
                     TGFCTVDFNIWPNFSRILLVTLMFVGGCAGSTSGGLKVARVLILLKMAYARLEQTFRP
                     KMVRVVRISGHVVDEGVQRTACSFFVLYLMWFMAGSLFMSLLGLPFQTAVTSVATTLN
                     NTGPGLGAIGAVENFSQLPTAGKAFLSLCMVLGRLELFSIMVLFVPSFWRRA"
     gene            complement(2769..>3208)
                     /locus_tag="PKW60_12575"
     CDS             complement(2769..>3208)
                     /locus_tag="PKW60_12575"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPK00313.1"
                     /translation="VNIMAGVLAKEIGATRAIAVVHEPDFASLVAKLGIDHVVTPRAC
                     LANRVLKLVHQDALQSIAVLEEGQVEIVEFAVDGEAPIIGKTLKEVFEPRRGHRGALV
                     GAIIRGDDVIVPRGSDGIQAGDSIIMIAAPEALDGVLKLFSKR"
BASE COUNT          718 a          997 c          891 g          602 t
ORIGIN      
        1 aaaagagcag atacgtttca ggcggcgcag cacattggcc aggtggctgc cgaacacgcc
       61 ccggtagtga tgcagctcgt cgatcaccac gtagcgcaga ttttcaaaaa gacggagcca
      121 cttggtgtga tgcggcagaa tcccggtgtg cagcatgtcc ggatttgtca caacaatatg
      181 cccagcctgc cggattgcct ttcgtgccga gccgggcgtg tcgccatcga aagtataggt
      241 gccgatcttg acctcgagat cctcgatggt gtcctgcagc tcatgcacct ggtcttgcga
      301 cagcgccttg gttgggaaga tatacagggc ccgggcctcg tgttcctgca tcaacgtgtt
      361 caaaaccggc acgttataac acaaggtctt cccggaggcc gttggagtca ctacgcagac
      421 gttttccccg gcgagagctg cctcgactgc ttcctgctgg tgcgtataca ggttctgtat
      481 cccgcgccgc tgaaggccct ggaccagctt cgggttcagg gcttcgggga atcctgcgta
      541 acgtgccggc tgtgctggaa cggtccgcca cgccgtcaaa tgccgtgtaa aatcggagtc
      601 cgaccgaagc cgatcaatga tctgggcaac gttcatgatg tctgggcagc actccctttc
      661 tttggcatca gcgcaagtca tctgaatatt acatcagtac atcagccatt gcaagaccac
      721 tgcgcagccc caaaaaccgc ttttgacaac acaaaacccg gccggtacca ggccggccgg
      781 gccatttttc cgaaaataac caatactgcc gaatcccgcc tattccgcgg cgtacatcat
      841 ccttcgtccc cagggagcgg cgcctctgcc tgcgggctgg ggggggccgc cggggcatct
      901 gctgatttcg tgcaaccggc aacgaacatt gacgcaaaga ccatcgttag cgccgcgaac
      961 gccatcaata cccgtctcat cttcatctcc tcccctggtt ggcatcggcc cgagaacgcg
     1021 gagcataccg cgctccccgc ctggcagctt cccttcaggc cgtgtggccc gaatttcaaa
     1081 gttctgataa gttagaatat tacgtttaag ataaaaagtc aaaaaatctg ccttttcgaa
     1141 aaagtgggca agctcgtccg ctgtttgcgc tcagctggcc gcatgcaagc ccactgcccg
     1201 gccgattgat ctccattgcg cccggaaagc atttggaaca gaacgagttg ccggtacggc
     1261 gccttacgcg cgccgccaga atgaggggac gaacagcacc atgatgctga acagctccag
     1321 gcgtccgagc accatgcaca gtgacaggaa tgccttgcct gctgtgggca gctgagagaa
     1381 gttctctacc gcaccgatgg cgccaagccc ggggccggta ttgttcaacg tggtggccac
     1441 cgacgtcacg gccgtctgaa acggcaaccc cagcagcgac atgaacaggc tgcctgccat
     1501 gaaccacatg aggtacagca caaaaaagct gcacgcggtg cgctgcacgc cctcgtccac
     1561 gacatgcccg ctgatgcgca cgacgcgcac cattttcggg cgaaacgtct gttcgagccg
     1621 ggcataggcc attttcagca agatcaggac ccgcgcgacc ttgagtccgc cgctggtcga
     1681 gcctgcgcag cctcccacaa acatcagcgt tacgaggagt atccgcgaga agttgggcca
     1741 gatattgaag tcgacggtgc aaaaccccgt ggtggtcatg acggatgcca cgttgaacga
     1801 cgcatgccgc agggcttctc ccgcggacgg aaagaacacc ttctcctggg gcggaagcgg
     1861 cgaatcaccc acaggaaatg acccttcagc tcccatgaga ttcacagtga tcgcgagcgt
     1921 gcaggcaccc agaatggcca ggtagaccag ccactcggta tctctgagca gcgctcgcca
     1981 gttccccgta aacagggcaa agtgcaacgc gaaattcgac cccgcgcata tcatgaaaaa
     2041 agtgagaacc atttcgatgg ccacactgtc ataggcgcca atgctcgctg cctgtgtcga
     2101 gaacccgccc gtgcccaatg tggcaaacgt atggcacaga gcatcaaaca ggctcatgcc
     2161 gcacagcatc agggcggcgg tttgcgcgat ggtgaagcca aagtagatct tgtacagaat
     2221 cgaggccgta tccttgatcc gcggtttcaa accgcgcgga tccggccccg gagattccga
     2281 tttaaacagt tgtttcccac ccgcgccaag gtacggcaga acggcaataa acagcacaac
     2341 aattcccata ccccccagcc aatgcgtgaa cgaacgccag aacatgatgc acttgggcgt
     2401 cgttgcgaaa cacgccgctg tcatcacggt ggacccggtt gtcgtgaatc ctgacatgca
     2461 ttcaaaatac gcgtccaccg ggttgctgaa cacgccgccg aatagatacg gcaaaccgcc
     2521 cagacccgcc agaagaatcc aggaaaggcc cacgaggccc aggccttcgc gctggtggaa
     2581 ctcagccggc gcgttgcggc cggccagcgt cagcagatat cccgtcagga aggaaacgcc
     2641 tgtgcccccc agcagcgccg tcaaacctcc ccattcccgg tacagtacgg cccacaacgc
     2701 cgaaggcagc atgagcaggc cgatggcgaa ggaaaaatgc ccgagatatt ttgtgacaag
     2761 ccggtaattc atcgcttgct gaagagtttg agtacgccgt ccagcgcttc cggagcagcg
     2821 atcatgatga ttgagtcccc cgcctggatc ccgtcgcttc cgcggggcac aatcacgtcg
     2881 tccccccgga tgatcgctcc gacaagcgct ccgcggtgcc cccggcgcgg ttcgaacact
     2941 tctttcagcg tcttgccgat gatcggagct tcgccgtcca cggcaaattc gactatctcc
     3001 acctggcctt cttcaagtac agcgatggat tggagcgcat cctgatgtac cagcttcagg
     3061 acgcggttgg ccaggcaggc gcggggagtc accacgtggt cgatgccgag tttggcgacc
     3121 agagaggcga aatcgggttc gtgtacgacg gcaatggccc gcgtcgcccc gatctctttc
     3181 gctaagaccc cggccatgat gttcactt
//
LOCUS       DAOPBQ010000444         3206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_23977_length_3206_cov_4.171057, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000444 DAOPBQ010000000
VERSION     DAOPBQ010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23977_length_3206_cov_4.171057"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKW60_12580"
     CDS             <1..245
                     /locus_tag="PKW60_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPK00314.1"
                     /translation="RILDHSPSSKLSFEDLVKAIGITRVHVIDPTENMKVFADLISEC
                     LDSGELAVVIARRPCLLAEQKQTALERAAASQSCTP"
     gene            242..757
                     /locus_tag="PKW60_12585"
     CDS             242..757
                     /locus_tag="PKW60_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPK00315.1"
                     /translation="MKPVTNVIIAGVGGQGVVKASDILTEAVFRAGYDVKKSEIHGMS
                     QRGGSVASDIRFGEQVLSPMVPPGEADYILVLTEDESGTVENQLKESGTLLSPESLTG
                     YIDEDADEEDDEETRLRLRCMNVALLGALSVFLEIPDSYWQEALYANLPKRAWEFNDK
                     VFQLGRELVDR"
     gene            820..2151
                     /locus_tag="PKW60_12590"
     CDS             820..2151
                     /locus_tag="PKW60_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPK00316.1"
                     /translation="MKHGKQTSGGGFNPVSAPDYLPLAQLRQLQLDRLKSIVKRAYDN
                     VLFHRQRMEEKGVTPGDIQSLEDITRLHFTMKDDLREAYPFGLFASPMEEVVRVHASS
                     GTTGKPTVAAYTQEDINVWTNVMVRSFISCGCHRGDIIQNAYGYGLFTGGLGAHYGAE
                     ALGATVIPISGGNTDRQIMIMKDFGSNAISCTPSYFVHLAERALEMGIDLRELPLRVG
                     VFGAEPWSDGLRQRAEEIGGIKAYDIYGLSEIIGPGVAIECAMQQGLHIFEDHFYPEI
                     IDPETCEPVAPGEEGELVLTTLSKVAMPLLRYRTRDITRMIPQTCECGRTICRMARIG
                     RRSDDMLIIRGVNVYPSQIEAALLKVEGTSPHYQIVLSRDKDLDQMEVQFEVTPEMFG
                     DTVGSVENLQRTLCQAIESTIGVRAKITLVEPHSIERSQGKAKRVIDKRGG"
     gene            2196..2633
                     /locus_tag="PKW60_12595"
     CDS             2196..2633
                     /locus_tag="PKW60_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPK00317.1"
                     /translation="MKLTQLSLFLENKPAHLKVPCQALADAGISIMTLSLADTEQFGI
                     LRLIVCDWQKAKEVLENAGCVVNTTEVVAVEVQDRPGGLAGLLEIIEKAGINIEYMYA
                     FTFGRGDQAILIFRFENPDEAVAKLQAAGVGVVDDISLYSRGT"
     gene            complement(2713..>3206)
                     /locus_tag="PKW60_12600"
     CDS             complement(2713..>3206)
                     /locus_tag="PKW60_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HPK00318.1"
                     /translation="FHPEGADPADWLPDEAPSAASALPVGDGMSDDHINFATAQDGTL
                     YAAVKTSYDTPGYPLIGLLVRRPSGAWDDLYSVDDEGTRPIVVLDEKSGTLFVAYTHA
                     GNIVYRESPSESIAFGPRHMLIEKKEAPLNNVTSTKERFSGEIVFLASTPATAEGVQL
                     RFP"
BASE COUNT          723 a          961 c          931 g          591 t
ORIGIN      
        1 ggagaatact tgaccattca ccgtcttcga aactatcgtt tgaagacttg gtgaaggcta
       61 tcggcatcac ccgggtccac gtgatcgacc cgacggaaaa tatgaaggta ttcgcagatt
      121 tgatctccga atgcctggac agcggtgaat tggcagtggt cattgcgcgc cggccctgcc
      181 ttctcgcgga acagaaacaa acggcactgg agcgcgcggc ggcctcacaa tcatgcacac
      241 catgaaacct gtaaccaatg tcatcattgc cggcgtgggc ggccagggcg tcgttaaagc
      301 ctccgacatc ctcaccgaag ccgtgttccg ggccggttac gacgtcaaaa agagcgagat
      361 ccacggaatg agccagcgcg gcggctccgt cgcaagcgac atccggtttg gcgaacaggt
      421 gctcagcccg atggttcctc cgggagaagc cgattacatc ctggtgctga ccgaggatga
      481 atcaggcacc gtcgaaaacc aattgaagga aagcggcacc ctgctcagtc ccgaaagcct
      541 gacggggtat attgatgaag atgccgacga agaggacgat gaggaaaccc ggctgcggct
      601 gcggtgcatg aatgtagccc tgctgggcgc cctcagtgtc ttcctggaaa ttccggactc
      661 ttactggcag gaagccttgt atgccaacct cccgaaacgg gcctgggaat tcaatgacaa
      721 agtgtttcaa ttgggccggg agctcgtaga ccgatagcgg ccggactctc cggcacgcaa
      781 gagtggcgga cgtgtagagc gtgaagcaag agggggcgta tgaaacacgg aaagcaaact
      841 tcaggagggg gattcaatcc cgtaagcgca cctgactatc tgccactggc ccaactgcgc
      901 cagctgcagc tggaccgcct gaagtccatc gtcaagcgcg catatgacaa cgtcctgttc
      961 caccggcaac ggatggaaga aaagggcgtc acgcccggcg acattcagtc gctggaggac
     1021 atcaccagac tgcatttcac catgaaagac gatttgcgag aagcgtatcc gttcggctta
     1081 ttcgcaagtc ccatggagga ggtcgtccgg gtacacgcct ccagcggcac caccggcaaa
     1141 ccgaccgtcg cggcatatac ccaggaagac atcaacgtgt ggaccaatgt catggtccgc
     1201 agctttattt cgtgcggctg ccaccggggc gacattatcc agaacgcgta cgggtatggc
     1261 ctgtttacag gcggcctggg cgcacattac ggcgccgaag ccctgggcgc caccgtaatc
     1321 cccatatcgg gcgggaacac cgaccgccag atcatgatca tgaaagactt cggctccaac
     1381 gccatttcct gcacacccag ctatttcgtc cacttggcgg aacgggcgct tgaaatgggg
     1441 attgacctgc gcgaactgcc gctgcgggtc ggcgtgttcg gggcggagcc ctggagtgat
     1501 ggactccgcc agcgcgccga agaaatcggc ggcatcaagg cctacgacat ctacggcctg
     1561 tccgagatta tcgggcccgg cgtcgctatc gaatgcgcga tgcagcaagg ccttcacatc
     1621 tttgaggacc acttctatcc tgagattatc gacccggaga cctgcgagcc cgtcgcgccg
     1681 ggtgaagaag gcgaactggt gctgacgacc ctcagcaaag tcgccatgcc tctgttgcgc
     1741 taccgcacac gcgatattac gcgcatgatc ccccagacct gcgagtgcgg gcgcaccatc
     1801 tgccgcatgg cccgcatcgg acgccgcagc gacgacatgc tcatcattcg cggcgtcaat
     1861 gtgtacccct cccaaatcga agcggccctg ctcaaagtgg aaggcacgtc gccgcactac
     1921 cagatcgtcc tctcgcgcga taaagacctg gaccagatgg aagtgcagtt cgaagttaca
     1981 cccgaaatgt tcggtgatac ggtggggtcc gtggaaaatc tccagcgaac cttgtgccag
     2041 gccatcgaaa gcaccatcgg cgtccgcgca aaaatcaccc tggtcgaacc ccattcgatt
     2101 gagcgcagcc agggcaaagc taaacgggtt atagacaaac ggggaggctg aaaccgaatg
     2161 ccggagaaac tccggcgcat gaggagaccg acacgatgaa gctcacgcaa ttgtccttgt
     2221 tcctcgaaaa caagccggct cacctcaagg taccgtgcca ggccctggcc gatgccggca
     2281 tcagcatcat gactctctcg cttgccgata ccgagcagtt tggcatcctc cgcctgatcg
     2341 tttgcgactg gcaaaaagcc aaggaagtgc ttgaaaacgc cggttgcgtc gtcaacacca
     2401 ccgaagtcgt cgccgtcgaa gtgcaagacc ggcccggcgg gctggccggc ctgctcgaaa
     2461 tcatcgaaaa ggcgggcatc aacatcgaat acatgtatgc gttcaccttt ggccgcggcg
     2521 atcaggccat cctcatcttc cgttttgaaa atcccgatga agccgtcgcc aaactccagg
     2581 ccgccggagt gggcgtcgtc gacgacatct ccctgtactc gcgcgggacg taagggaccc
     2641 tgactgaaaa aacaccggcc gcccccgcga acacaggggt ttgcgggggc ggtttttgtt
     2701 gcatcaaagg catcagggga agcgtagctg caccccttca gccgtggcgg gtgtcgaagc
     2761 gaggaacaca atttcaccgc tgaaccgctc ttttgtgctc gtcacattat tgagcggggc
     2821 ctcttttttc tctatcagca tgtgacgggg cccgaatgca atggactcgc tcggcgattc
     2881 tctgtagaca atgttcccag catgcgtgta cgccacaaaa agcgtgccgc tcttctcgtc
     2941 caggaccacg atggggcgcg ttccttcatc atcaacacta tacagatcgt cccaggcgcc
     3001 ggacggccgc cgtaccagca ggccgatgag cgggtaccct ggcgtgtcgt aactggtttt
     3061 cacggccgca taaagcgtcc cgtcctgagc cgtcgcaaaa tttatgtgat catccgacat
     3121 gccgtcccca acgggcaggg cggaggctgc gctgggggct tcatccggga gccagtccgc
     3181 ggggtcagcg ccttcaggat gaaaac
//
LOCUS       DAOPBQ010000445         3196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24062_length_3196_cov_4.128940, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000445 DAOPBQ010000000
VERSION     DAOPBQ010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24062_length_3196_cov_4.128940"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PKW60_12605"
     CDS             <1..690
                     /locus_tag="PKW60_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00319.1"
                     /translation="APEMAPTTLRLYILGAGLNIALFQRGLWVFHGSVVCIDGKAAAF
                     LGHPHAGKTTIAAALHRRGHTLLSDDVLAIRMTEDGPLALPGFPRFKLWPDSAGALGV
                     ESETLPRIKEDTDKRSCAVARRFREEPAPLTNVYILNEGPELEMTRQVPKQAQLDLIP
                     YWDLSKFGLAFLQGPRMGACFLQCGGLAKQASVSVLTRTTDLADLPRLAEMIEDDVAA
                     DPARYEYSGAV"
     gene            668..1726
                     /locus_tag="PKW60_12610"
     CDS             668..1726
                     /locus_tag="PKW60_12610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK00320.1"
                     /translation="MSTVGRYEGMPAVSVIIPTRNRPHLLCEAIQSVLDQTFSDFELL
                     VVDDGSEAPVDAEIETHADPRIRVIRQAHAGRSAARNRGLALATGKYIAFLDDDDTYL
                     PNKLAEQVQFLETNPGESIVSAGAQYVDEGGRELGEYAPWTDGRILTFSDAMYGTRMI
                     TSTVLFRRGLLDQMDAWFDPEMDLAEDGDFFLRLLYCAGQATLLPSRVSTYRLRDDDC
                     TGGGPRASEGYRRVIDKAFRLPDLPKDVVNNRDRIYAHLYLICGSRAFAVRDPVHGRE
                     QLEAALKLDAVYVEAELPVTLARFAGFRWSDPRRYVEYALENLPASLAPLRARKKEVY
                     QAFLQQQAAPFTTARHEG"
     gene            1731..2783
                     /locus_tag="PKW60_12615"
     CDS             1731..2783
                     /locus_tag="PKW60_12615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK00321.1"
                     /translation="MPEVSVIIPTYNRSGFVVEAVNSVLSQTFDDLEVVVVNDGSSDE
                     TARVLENFNDPRVKPMHQANVGRSTARNRGFAESSGQFIVFLDDDDLFLPEKLEKQVN
                     FLRENPDTGLVASGFNYVAHDEQIIQTAHPWKHVPVLGWRSCILEIGALHLSAVLVRR
                     SAVLNLDVPFHDGLEACEDTDFFVRLSLTGCEMKWLQDVVCSYRQHASNTMGSLGGPR
                     YREVIGWLLQRWFARDDLPPEMRAEQAEIEAYFDLVCALRAYAREEIEAGQRLLVAAL
                     KTCPEWETTMFPEFVARFVGYLHADPRIMIERVFGKLPGEYAHLAGLKREAYRSFLSK
                     VVTAAQDGEGPLGESR"
     gene            2770..>3196
                     /locus_tag="PKW60_12620"
     CDS             2770..>3196
                     /locus_tag="PKW60_12620"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPK00322.1"
                     /translation="MSLAELKEERLLGLPVSREAEVLLCAASVRPAAERIDRLRALLT
                     QDLDWDWLREAAAHHCIESMLYWHLSRAGLDAVPENATAALKQSYARVVQCNMLFVQE
                     LLHLVKLLSDASIPVVPYKGPLLASVLYEDLAQRQMWDLD"
BASE COUNT          624 a          897 c         1010 g          665 t
ORIGIN      
        1 gcgcccgaga tggcgcccac aacgctgcgc ctgtatattt tgggcgcagg attgaacatc
       61 gccctgttcc agcgcgggtt atgggtgttt cacgggagtg tggtgtgcat cgacgggaaa
      121 gcggcggcct tcctgggcca cccgcacgcg ggaaaaacca ccatcgcggc ggccctgcac
      181 cgccgcggcc acacgttgct gtcggatgat gtgctggcga tccgcatgac ggaggatggc
      241 ccgctggcgc tccccggatt tccccgtttc aagctgtggc cggattccgc cggagccctg
      301 ggggttgagt cggagacgct gccccggatt aaagaagaca cggacaagcg gtcctgcgcc
      361 gtggcgcgcc gttttcgaga ggagccggcc ccgctgacca acgtgtacat cctgaatgaa
      421 gggcccgaac ttgagatgac gcgccaggtc cccaaacagg cacagcttga cctcatcccg
      481 tattgggatc tgtcgaaatt tggcctggca ttcctccagg gcccgcgcat gggggcctgc
      541 tttctccaat gcggtgggct ggctaagcag gcctcggtgt ctgtgctcac ccggacgacc
      601 gatctggctg atctccctcg cctcgctgaa atgatcgaag acgatgtggc ggccgatccg
      661 gcacggtatg agtacagtgg ggcggtatga aggcatgccg gccgtaagcg tcatcatccc
      721 gactcgcaac cgtccccacc tcctatgtga agccatccag agcgtgctcg accagacgtt
      781 cagcgatttt gaattgctcg tggtggacga cggcagcgaa gcacctgtcg acgcggagat
      841 cgagacccat gccgatccgc gcatccgcgt gatccgccaa gcgcatgccg ggcggagtgc
      901 cgctcgcaac cggggactgg cgcttgccac tgggaagtac atcgcatttc ttgacgacga
      961 tgacacctat ctgcccaata aactcgccga acaggtgcag tttctcgaga cgaaccccgg
     1021 cgagagcatt gtgtctgcgg gtgctcaata cgtggacgaa gggggccgtg agctggggga
     1081 gtatgccccc tggaccgacg gacgcatcct caccttttcc gacgccatgt atggcacgcg
     1141 gatgatcacc agtacggtgc tcttccggcg gggcctgctg gaccaaatgg acgcgtggtt
     1201 tgacccggaa atggatttgg cggaggacgg agactttttc ctgaggctgc tgtattgcgc
     1261 ggggcaggca acgctgttgc cgagccgggt aagcacctac cgactgcgcg acgatgattg
     1321 cacaggcggc gggcccaggg ccagcgaagg ctaccgccga gttattgaca aagcattccg
     1381 tctgccggat ctgccgaagg atgtcgtgaa caatcgcgat cggatctacg cccatctcta
     1441 cctgatctgt ggatcccggg cgtttgccgt tcgggatcca gttcacggtc gggaacaact
     1501 ggaggccgcg ttgaaactgg acgcggtcta cgtggaagcc gaactgcctg tgacccttgc
     1561 ccggttcgcg gggtttcgct ggagcgatcc caggcggtac gttgagtatg ccttggaaaa
     1621 cctccccgcc tctctggcgc cgttacgcgc tcgaaagaaa gaagtctacc aggcctttct
     1681 gcagcaacag gcggcgccgt tcacaacggc gagacacgaa ggatgaacag atgcccgaag
     1741 tcagcgtgat catccccacc tataatcgat ccggcttcgt ggttgaggcg gtaaacagcg
     1801 ttttgagtca aacatttgac gacttggagg ttgtggtcgt caacgatggc agttccgacg
     1861 aaaccgcccg agttttggaa aatttcaacg atccgcgcgt aaaaccgatg catcaggcca
     1921 atgtggggcg cagcaccgcc cgaaaccgcg gatttgcaga atcatccggc caattcattg
     1981 tttttcttga cgacgacgac ctttttttgc ccgaaaagct ggaaaagcag gtgaattttc
     2041 tgcgagaaaa ccccgacaca ggtctggtgg cctcgggctt caattatgtg gctcacgatg
     2101 aacaaattat tcagactgcc cacccctgga agcatgtgcc tgtgcttggc tggcgcagtt
     2161 gtatcctgga aattggcgcg ctccaccttt ccgctgtctt ggttcggcgg tcggctgtct
     2221 tgaatttgga tgttccattt cacgatggtt tggaagcatg tgaagacacg gatttttttg
     2281 tgcggctcag cctgacaggc tgtgagatga aatggcttca agacgttgtc tgctcgtata
     2341 gacagcatgc gtccaacacc atggggagtc ttggcggtcc ccggtatcgg gaagttattg
     2401 gctggctgct ccagagatgg tttgcccgtg acgatctgcc gcctgaaatg cgtgcggagc
     2461 aggcagagat tgaagcctat tttgatttgg tgtgtgcatt gcgcgcctat gcccgcgagg
     2521 agatagaagc cgggcagcgc cttctggtag cggccttgaa gacctgcccg gaatgggaga
     2581 ccacgatgtt tccggagttc gtggctcggt tcgtgggcta tctccatgca gatccgcgga
     2641 tcatgatcga gcgggtgttc gggaagctcc cgggggaata tgcccacctt gcagggctga
     2701 agcgggaagc ctaccgatcg tttctctcaa aggtagtcac cgcagcacag gatggggagg
     2761 gcccgctggg tgagtctcgc tgaattgaag gaagagcggc tgctgggcct gccggtatcg
     2821 cgggaagccg aggtgctgct gtgcgcagca tccgtaaggc ctgcggcgga gcgcattgac
     2881 cgcctgcgcg ccctcctgac acaagatttg gactgggatt ggcttcgtga ggccgcagcg
     2941 catcattgca tagaatccat gctctattgg cacctgagca gggccgggct ggacgcggtg
     3001 ccggaaaacg ccacggcagc gctgaaacaa agctatgccc gcgttgtgca gtgcaacatg
     3061 ctgtttgttc aggagctgct tcatctggtg aagctgctga gcgacgcctc tattccggtg
     3121 gtgccataca aagggccgct gctcgcgagc gtgttatacg aagaccttgc gcaacgccag
     3181 atgtgggacc tggatc
//
LOCUS       DAOPBQ010000446         3188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24129_length_3188_cov_3.340568, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000446 DAOPBQ010000000
VERSION     DAOPBQ010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24129_length_3188_cov_3.340568"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PKW60_12625"
     CDS             <1..450
                     /locus_tag="PKW60_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HPK00323.1"
                     /translation="WVERVEVAGEGGTRTIAKEPSLYDYRRQSFCPPGDLILQAIFQF
                     RAAETDQRAIYEHYLQRRREKQPQGNCCGSVFKNPPNGHAGRLIEACGLKGTRRGGAL
                     VSPVHANFIMNDGGATFDDIRSLIRLCQSTVKERFGVNLEREVVVLD"
     gene            complement(480..788)
                     /locus_tag="PKW60_12630"
     CDS             complement(480..788)
                     /locus_tag="PKW60_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPK00324.1"
                     /translation="MLEHALPYKKIIFVCVNQRPPGERVCCASAGGLVLYARLKNMIK
                     DRGLQGRIRVAKSGCLDRCEDGPNIMVFPDNVWYSHVAAADLEPLVARLTGSLGAEDQ
                     "
     gene            986..2419
                     /gene="hisS"
                     /locus_tag="PKW60_12635"
     CDS             986..2419
                     /gene="hisS"
                     /locus_tag="PKW60_12635"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPK00325.1"
                     /translation="MPDDKPLIEPRVLKGFQDFLPEEVLARKAVMEVCESVFRKYGFV
                     PLQTPALEYLDALLGTGGEETNKELFRLETPEGELVAMRFDQTVPFARMMAQYPDKLK
                     APFRRYAMGPVWRGDRPGPGRFREFTQIDIDAAGSESVAVDAEIIAVVCEIMEKLGAK
                     DFCVLINNRKLIDALLEECGIAGEARGKHVLRVIDKLAKVGVDLVRQELGSGRIDESG
                     DPIPGVHLDAKTIDAILSFIAITGATREEVLEAMREALPASARAAEALDEMAQLAACL
                     DALGVPAAQARFAPNLARGLDYYTGPVYEIAIAEGRFGSVGGGGRYDGLVSRFLERKI
                     PCAGIAFGLDRLMAALQFLGISPQVSAQPQVLVASVGRVPEAELLRLAQELRGAGLRA
                     EVYFSAKRGMKHQLAHADRCCIPVAVIAGEDELAKGVVSIKDLLAGKDQREDISDHDA
                     YRKAGRTAQVTVARDEMVRTIHEILAS"
     gene            2496..>3188
                     /locus_tag="PKW60_12640"
     CDS             2496..>3188
                     /locus_tag="PKW60_12640"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK00326.1"
                     /translation="MTGRERILAMLEGRDVDHLPLMPITMMFAADLVGVKYGDYARNG
                     EVLAEAQLRVAEAFGFDYVSAISDPAREAADCGAAIQWFEDQPPAVDESQALLQHPED
                     LLHLAIPDPLGDGRMHDRVKAISLLKERAGRDRIVEGWVEGPIAEAADLRGINHIMMD
                     FFEQPGFVRDLFDFVIEMELAFAQAQKDAGADLMGVGDAAASLVGPQLYEEFVWPAEK
                     RLVDGLHAMGLNV"
BASE COUNT          609 a          920 c         1081 g          578 t
ORIGIN      
        1 tgggtcgaga gagtcgaagt ggccggagaa ggcggcaccc ggacgatcgc gaaggagccg
       61 tcgctttacg actaccggcg ccagtcattc tgtccgccgg gggacctgat cttacaggca
      121 atcttccagt ttcgcgcagc ggagaccgat cagcgggcga tatacgagca ttacctgcag
      181 cgccgccgcg aaaaacagcc acagggtaac tgctgcggca gcgtcttcaa gaatccgccg
      241 aatgggcacg caggacgcct cattgaagct tgcggattga aagggacgcg gcgaggcggc
      301 gccctggtga gcccggtgca cgcaaatttc atcatgaacg acggcggggc gacgtttgac
      361 gatatccgca gcctgatccg gctctgccag agcacggtga aggagcggtt cggcgtgaac
      421 ctcgagcgcg aggtcgtggt gctcgactga gacggcgttc tgccgtggta atcccgagat
      481 tattggtctt cggcgccaag ggaaccggta agccgcgcca ccaggggttc gaggtcggcc
      541 gcggccacgt gcgaatacca cacattatca gggaacacca tgatgttggg cccgtcttcg
      601 cagcggtcca gacagccgct tttggccacc cggatgcgtc cctgcaggcc gcggtctttg
      661 atcatgtttt tgagccgggc ataaaggacg agcccgccgg cgctcgcgca gcatacgcgt
      721 tcgccgggcg gccgctggtt gacgcacaca aagatgatct tcttgtaggg cagcgcgtgt
      781 tcaagcatgg ccacacctct acgattcgct ccaaaggact gtcataccag agcagaggcc
      841 ggcgaatcca gtttttcgac aatcccgcct gacgccctcc aggtggtatc aggaaaaccg
      901 ggcacatttt gaaggctttt gccggttgcg tgtacactct ggccatcatt caaacccaat
      961 tcgcttgaat ctgcgaggaa acaggatgcc tgacgacaag ccgttgatag agccgcgtgt
     1021 tttgaagggg tttcaggact ttctgccgga agaggtcctg gcccggaagg ccgttatgga
     1081 agtatgcgaa tcggtttttc gcaagtacgg ttttgtgcct ctgcagacac cggcgctcga
     1141 atatcttgat gcactgctgg gcacgggcgg tgaagagacc aataaagagc tgttccgtct
     1201 ggaaacgccc gaaggcgagc tcgtggcgat gcgcttcgac cagacggtgc cgttcgcgcg
     1261 catgatggcg cagtatccgg acaagctgaa agccccgttc cggcgctatg cgatggggcc
     1321 cgtatggcgg ggagaccggc cggggcccgg gcgttttcgc gagtttaccc agatcgatat
     1381 tgacgcggcc ggctcggaat cggtcgcggt cgacgcggaa atcatcgcgg tggtatgcga
     1441 gatcatggaa aaactgggcg cgaaggattt ctgcgtcctg atcaacaatc gcaagctcat
     1501 cgatgccctg ctggaggagt gcggcattgc cggcgaggca cgcggaaaac acgtgctgcg
     1561 ggtcatcgac aaactcgcga aagtgggcgt cgatctggtc cgccaggaac tgggcagcgg
     1621 gcgcatcgac gagtcgggcg atccgatccc cggcgtgcat ctggacgcga aaaccatcga
     1681 tgcgatcctg tcgttcatcg cgatcaccgg cgccacccgc gaggaagtgc ttgaagcgat
     1741 gcgggaggcg ctcccggcga gcgcgcgggc ggcggaagca ctggacgaga tggcgcagct
     1801 tgcggcctgt ctcgacgcgc tgggcgttcc ggcggctcag gcgcggttcg cgcccaacct
     1861 tgcacggggg ctggattact acaccggtcc agtgtacgag atcgcgatcg cagaggggcg
     1921 ctttggctca gtcggtggcg gcggccgcta cgacggtctg gtgtcgcgat ttctcgagcg
     1981 caagatcccc tgcgccggca tcgcatttgg gctggatcgg ctgatggccg cgctccagtt
     2041 tctgggaata agcccgcagg tatcggcgca accccaggtg ctggtggcgt ccgtagggcg
     2101 cgtgcccgaa gcggagttgc tccggcttgc acaggagctg cgcggggccg ggctccgtgc
     2161 ggaagtctat ttcagcgcca agcgcggcat gaaacatcag ttggcgcatg ccgatcggtg
     2221 ctgcattccg gtggcggtga tcgccggtga agacgaactg gcgaagggag tcgtgtcgat
     2281 caaagatctt ctggcgggaa aggaccagcg cgaggacatc tccgaccacg acgcctaccg
     2341 caaagccggc cgcaccgcac aggtcaccgt ggcgcgcgac gaaatggtgc ggacgattca
     2401 cgaaattctg gcatcgtgac ccgcctgcgc gagtaccggc acgaaaagcg cccggataag
     2461 cgcaccgtgt ggccgtaacg aaggaggacg ccgtgatgac cggtcgggaa cggattctgg
     2521 cgatgcttga agggcgcgac gtggaccacc tgccgctgat gccgatcacg atgatgttcg
     2581 cggcggacct ggtgggcgtg aaatatggcg actatgcacg aaacggcgag gtgctcgcgg
     2641 aagcgcagct gcgcgtcgcc gaggcgttcg gcttcgatta cgtctccgcc atttcggatc
     2701 ccgcccgcga agcggcggat tgcggcgcgg cgatccaatg gtttgaagat cagccgcctg
     2761 cggtggatga aagccaggcc ttgttgcagc acccggaaga cctgcttcac ctggccattc
     2821 cggatccgct gggcgacgga cggatgcacg accgcgtgaa agccatctcg ctgctcaaag
     2881 aacgcgcggg ccgcgatcgc attgttgaag gctgggtaga gggtcccatt gccgaggcgg
     2941 ccgatctgcg tggaatcaac cacatcatga tggatttttt cgaacagccc gggttcgtgc
     3001 gcgacctctt cgacttcgtc atcgagatgg aactcgcctt tgcgcaggcc cagaaagatg
     3061 ccggggccga tctcatgggc gtgggcgatg ccgcggcgtc ccttgtcggg ccgcagcttt
     3121 atgaggagtt cgtgtggccg gccgagaagc ggctcgtcga cggcctgcac gccatgggcc
     3181 tgaacgtg
//
LOCUS       DAOPBQ010000447         3186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24145_length_3186_cov_3.648036, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000447 DAOPBQ010000000
VERSION     DAOPBQ010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24145_length_3186_cov_3.648036"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1146)
                     /gene="mtnA"
                     /locus_tag="PKW60_12645"
     CDS             complement(85..1146)
                     /gene="mtnA"
                     /locus_tag="PKW60_12645"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPK00327.1"
                     /translation="MHLRPVIWRDGHLIIIDQTLLPAECREIRLASLESVREAICTLR
                     VRGAPAIGVCAAFGLLVALDEAGPANLAGGLQVLDAAAERLGSSRPTAVNLFWALRRM
                     QNAAQRIAAQTASLREWRERLESEACALRDEDTACCQRIGDYGATLIPEGAGVLTHCN
                     AGALATSAYGTALAAMYRAHEAGRTFHVFVDETRPLLQGARLTAWELLRAGIEVTLIC
                     DNTAAQVMREGRVQLVITGADRIAANGDTANKIGTYGLAVLAYAHGIPFYVAAPLSTF
                     DIDLPGGDHIPIEQRAGEEVTHTFGRPTAPEGVAVYNPAFDVTPARYIAGLVTEQGIL
                     MPPFEEAIRDCFENRAETP"
     gene            1600..2523
                     /locus_tag="PKW60_12650"
     CDS             1600..2523
                     /locus_tag="PKW60_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HPK00328.1"
                     /translation="MRNRGFTLIELLVVIAIIGILAAILLPALARAREAARRASCANN
                     LKQLGLILKMYANESPGEKFPPLRFRDGPACDEVTREAILIWAPDGGALYPDYLSDPQ
                     VLLCPSDPDITEVLERRAFYCSSDPGAGVCPCMMYPISYFYYGWALTPAHYLAAPDAL
                     QSTDPMLSFLSYDFLLAFAGLVGGVVGAPALQEALAAADRDVPIGAMTAYRLREGVER
                     FLVTDINNPSAGTRAQSELMVMHDSANAAVTVSGVDLLGNPFTVSEFNHVPGGGNVLF
                     MDGHVAFMRYPGEFPICGSWSMLFAQVRNII"
     gene            2657..>3186
                     /locus_tag="PKW60_12655"
     CDS             2657..>3186
                     /locus_tag="PKW60_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPK00329.1"
                     /translation="MSVWTAMGFDDLGIAPSLLSVLETLGIETPTPVQREAIPVALAH
                     RDLVAVAQTGTGKTLAYALPALMRLSDGPVGRNLMLVLTPTRELAQQVHGVFDVLGRP
                     LGIRSTAIYGGVGYEAQATALKRGVSIVVATPGRLLDHMRQGRAHFGDLMILVLDEAD
                     RMLDMGFLPDIRSIIQ"
BASE COUNT          605 a          907 c         1037 g          637 t
ORIGIN      
        1 gcatcctcga gggagatgcc gtttatccgg tcctccgcag tcttggcgat aatatctacc
       61 agcttttcga acataggccg agtctcatgg tgtctctgca cgattctcga agcagtcacg
      121 gatggcctcc tcaaaaggcg gcatgaggat accctgttcc gtgacgagcc ccgcgatgta
      181 acgggcggga gtgacgtcaa acgcaggatt gtagaccgcg acgccctcag gggccgtggg
      241 gcggccgaac gtgtgggtga cctcttcccc ggctcgctgc tcaatgggaa tgtggtctcc
      301 gccggggagg tcaatatcga aggtcgaaag cggtgcggca acgtagaagg ggatgccgtg
      361 ggcataggcg agaacagcca gcccgtaggt gccgatcttg ttggcggtat caccgttggc
      421 ggcgatgcgg tcggccccgg tgatcacgag ctgcacgcgg ccttcgcgca tgacttgggc
      481 agctgtgttg tcacagataa gcgtgacctc gataccggcg cgcaggagtt cccacgcggt
      541 gaggcgggcg ccctgcaaca ggggacgggt ctcatcgaca aagacgtgga aggtccggcc
      601 ggcttcgtgc gcgcggtaca tggcggccag cgcggtaccg taggcgctgg tggcgagggc
      661 gcccgcattg caatgcgtga gcactccggc gccctcgggg atcaacgttg ctccgtaatc
      721 gccgatccgc tggcaacagg cggtatcttc atcgcgaagc gcgcaggctt cgctttcaag
      781 ccgctcacgc cattcgcgca gggaagcagt ctgggcggca atcctctgcg ccgcgttctg
      841 catgcgccgc agggcccaga acaggttgac ggccgtcggg cgggaagatc ccaggcgttc
      901 ggcggcggcg tccagcacct gcaggccgcc ggccaggttc gccggcccgg cctcatccag
      961 cgcgaccaga aggccgaaag cggcgcagac gccgatcgcg ggggcgccac ggacccgaag
     1021 cgtgcaaatg gcctcgcgga cggattccag cgatgccagg cgaatctcgc ggcactccgc
     1081 cggaagcagc gtctgatcga tgatgatcaa gtgcccgtcg cgccaaatca ccggcctaag
     1141 atgcatcagg acaagctcct atttttgacc acactgtggg taggctacct tagtgcgcag
     1201 cctgaggcaa gctcaggggc aagacaattc attgtgatgg cgcggaaaag gcggcggcac
     1261 agcacaggcg tggatcgtcg caattccagg gcgcggcgcc gtgatccttt ccactttcgc
     1321 ggcaagcagg tggcaaattg gctccgtttg attcatatat ttctttttgt tgtcacgtga
     1381 tatgtgttat aaacaagtga acggtaaaga ggatccgcag gaaaaaccaa gtctttaaac
     1441 tagtaaattt ccattatata catagatttt gcgggttacg cgggatttac ttggcaaaat
     1501 ccttgctttt gcggtatctt gtagtaaaat ggggcttgtg ataaccatag acaacacctg
     1561 cgactgaatt gcagccaccc tgccggaagg aatacgccaa tgaggaatcg tggattcacg
     1621 ttgattgaat tgctggtagt aatcgccatc attggaattc tcgcagccat tctacttcca
     1681 gcccttgcgc gtgcgcggga ggccgcccgg cgcgcaagtt gtgccaataa cctgaagcag
     1741 ctgggattga ttttgaagat gtatgcgaat gaatcgcccg gcgagaaatt tccgccgctc
     1801 cggttccggg acgggccggc atgcgacgag gtgacccgcg aagcgatttt gatctgggcg
     1861 cctgatggag gagcgctcta ccccgactac ctgagcgatc cgcaggtgtt gctctgcccg
     1921 tcggatccgg atattactga ggtgttggaa cgccgggcct tctattgcag cagcgacccg
     1981 ggcgcggggg tatgtccgtg catgatgtat cccatttcgt acttttacta cgggtgggcc
     2041 ttgacgccgg cacactatct ggcagccccc gacgcactgc agagcaccga tccgatgctg
     2101 agcttcctga gttacgattt cctcctggcc ttcgccggcc tggttggggg cgtggttggg
     2161 gcgccggcgt tacaggaggc cctggcggca gcggatcgcg acgtgcccat aggcgcgatg
     2221 accgcgtacc gcttgcgcga aggggtcgag cgcttcctgg tcaccgacat caacaacccg
     2281 tcagccggaa cgcgggcgca aagtgaattg atggtgatgc acgactcagc gaatgcggct
     2341 gtgacggtga gcggggtcga cttgcttgga aatccgttca cggtaagcga attcaaccat
     2401 gttccgggcg gtgggaacgt gctttttatg gacggccacg tcgcgtttat gcggtatccg
     2461 ggagagttcc cgatctgtgg gtcctggtcg atgcttttcg ctcaagtgcg aaatattata
     2521 taatcacatc agacagcttc gactgccgga atgttctgcc gctcagcgcc caccttcggg
     2581 ctgaccggcc ggttgcaggc gtctgcgagc ctggccgcgc agagaccggc cactgcatgc
     2641 attccggttt caacccttga gtgtatggac cgcgatggga tttgatgatc tcggaatagc
     2701 tcccagtctg ttgagcgtgt tagagaccct gggaattgag acacctacgc cggtacaacg
     2761 ggaagcgatt ccggtggcct tggcgcaccg ggaccttgtc gccgtggcgc agacgggcac
     2821 gggcaagacc ctggcgtatg cgctgccggc gctgatgcgc ctctcggacg gccccgtggg
     2881 gcggaacctg atgctggtgc tgacgccgac tcgcgaactg gcgcagcagg tgcacggggt
     2941 gttcgacgtg ctgggccggc ccctgggcat tcgcagcacg gccatctacg gcggggtcgg
     3001 ttacgaagca caggccaccg cattgaaacg gggcgtatcc atcgtggtgg caacgccggg
     3061 ccgcctgctg gaccacatgc ggcagggccg ggcccacttc ggcgatctga tgatccttgt
     3121 tctggacgag gcggaccgga tgctcgacat ggggtttctg ccggacatcc ggagcatcat
     3181 ccagaa
//
LOCUS       DAOPBQ010000448         3175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24251_length_3175_cov_4.506731, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000448 DAOPBQ010000000
VERSION     DAOPBQ010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24251_length_3175_cov_4.506731"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3175)
                     /locus_tag="PKW60_12660"
     CDS             complement(<1..>3175)
                     /locus_tag="PKW60_12660"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPK00330.1"
                     /translation="LQPYEGDAIIFQDLSRIAGGGTITSWAWDFGDGTTSAAPAPSHA
                     YDSAGSFDITLTVQAGDESATLTKHKFITVRPRVAPIADFSALETVVPVGSPVVFADA
                     SAPGTANITSWQWSFGDGGTAAGAAQTHTYASPGSYTVSLAVTSPHGQDEAIKHNYIT
                     VNAIPPEAGFTCSDLQPFLRTAVSFSDISQPGHAPIASWFWEFGDGQTSTDQNPTHIY
                     EVAGPMTVSLTVADAQGTSNTNTQAAFLTVRGKPPVARFDMSPPWGVTIDEFEFTNAS
                     DEGTGDVSYLWRFGDGTESTEENPRHIYAEPGTYTVSLRAGNPFGHQFAQRVVRVHAT
                     EALDRYVRKEDPGISLVPVEQSSQPVPGGRWVVLSLVSQRWRSSAEVNPDVWQHWLAL
                     AIPDEVTNDTALLFISGGSTAQLAPEPKPELVDFAVRSGSVVAMLRMVPNQPTVFTEE
                     GLPRTEDAIIAFTFDKYMNSAAMGDPDEEWPALLPMTKSAVRAMDAVQAYTASLDNPI
                     AVKDFVVTGASKRGWTTWLTAAADSRVVGIAPMVIDMLNLAAQMDWHYSALCGYSEQL
                     QDYLDLGVIARLHTLEGTSLLSIVDPYSYVPRLNIPKYGLNGTSDDFFVPDSAQFYYG
                     DAAEFGSMHLRYFPNGNHDLLASREATVRGILPFYNAVVHKETLPRVEWNILEFPNSA
                     TLDVMLSSVPLSARLWTGTVSGGPGARDFGSHKVGNEVWKSTPITSDDAEGRFFSANV
                     PAPADGWTGYFMAFEFPSSTPGEPHIFTTEVRCVPTGNPCYAFPGGSGTKEIVGEGSD
                     RVTVVRLSGNRYQMGFWYGYLLADQIDAVWSAFSALIESGAEFPAGALEQAVSQLWKS
                     QYFDTAAWQTEFEGIARGCQAAGHPGITVDVLKKIIVIPDLSEYNCSLFAAWGEATAG
                     NQMFQLRNLDWTMDLGIQDYPVVAIYDPDDGLKHAVIGFAGLVGIAGGGMNEQGIAVS
                     EIMGYFCDEEYLAGIPFPVLLRDVLYHDRTLTEALTRMQNATRTNQYHYCVGDPNAED
                     PKARLLFTSRERFDMWADNESVTGQHPC"
BASE COUNT          612 a          922 c         1008 g          633 t
ORIGIN      
        1 ggcagggatg ctgccccgta acggattcgt tgtccgccca catatcgaag cgctcccggc
       61 tcgtgaagag caggcgggcc ttcgggtctt cggcattcgg gtcgcctacg caataatggt
      121 actgatttgt cctggtggca ttctgcatgc gcgtcagggc ctctgtcaac gtgcggtcgt
      181 gatagagcac atcgcgcagg agcacgggga acggtatccc cgcgagatat tcctcgtcac
      241 agaaataccc catgatctca ctaaccgcaa ttccctgctc attcattccg ccgccggcga
      301 tgcccaccag gcccgcaaac ccgatgacgg cgtgtttcaa accgtcgtca gggtcgtata
      361 tggccaccac cggataatcc tgtatgccca gatccatggt ccagtccagg ttgcggagct
      421 ggaacatctg gttgccggcc gtagcctcgc cccaggcggc gaagagcgag caattgtatt
      481 ccgacaggtc ggggatgacg attattttct taagcacgtc cacggtaatg ccgggatgtc
      541 ccgcggcctg gcagccgcgg gcaatgccct cgaattcggt ttgccaggcg gcggtatcga
      601 agtattgcga tttccagagc tggcttacgg cctgttcgag cgcacccgcc gggaactctg
      661 ctccgctttc gatgagcgcc gagaatgccg accacacggc gtcaatctgg tcggcgagca
      721 ggtacccgta ccagaatccc atctggtacc ggttaccact caggcggacc actgtgaccc
      781 gatcgcttcc ttctccaacg atttcttttg tgccggaacc gcccggaaac gcatagcatg
      841 gattccctgt gggcacgcag cgtacttcgg tggtgaagat gtgcggctca cccggcgtgc
      901 tgctggggaa ctcaaaggcc atgaaatagc cggtccatcc atccgccggg gcgggcacat
      961 tcgccgagaa aaaccggcct tcggcatcgt cagaggtaat cggcgtcgat ttccagactt
     1021 cgtttcctac cttgtgggac ccgaagtccc gggcgccggg tcctccgctc accgtgccgg
     1081 tccacaagcg ggcggacagg ggcacggagc tcagcatcac gtcgagtgta gcggagttgg
     1141 gaaactccag gatgttccac tccacccggg gaagcgtttc tttatggacg acggcgttat
     1201 agaagggcag gatgccccgc acggtagctt cccggctggc gaggaggtcg tggttgccgt
     1261 tggggaaata ccgcaaatgc atggagccga actcggcggc atcgccgtag taaaactggg
     1321 ctgagtcggg cacaaagaag tcgtcggaag tgccgttcag tccgtacttc gggatgttga
     1381 gccgcggcac gtacgagtag gggtccacta tggacaggag ggaggttcct tcgagcgtat
     1441 gcaaccgcgc gatcacgccc aggtccaggt aatcctgcag ctgctcggaa tacccgcaca
     1501 gggcggaata gtgccagtcc atctgggcgg cgaggttgag catgtcaatg accatcggtg
     1561 cgatgccaac gacccgcgaa tccgccgcgg ccgtaagcca ggtggtccat ccccgcttag
     1621 acgcgccggt cacgacgaaa tcttttaccg cgatggggtt gtccagagag gccgtatacg
     1681 cctgtacggc gtccattgcg cgtacggcac tcttggtcat ggggagcaac gcgggccatt
     1741 cttcatcggg atcgcccatg gcggcgctgt tcatgtactt gtcgaaggtg aacgcgatga
     1801 tcgcgtcctc cgttcggggc aggccttctt ctgtaaacac ggtcggctgg ttggggacca
     1861 tacgcaacat agcgacgaca gagcctgacc gcacggcgaa atcgacaagc tccggcttgg
     1921 gttccggcgc caattgtgcg gtgcttcctc ccgagatgaa gaggagcgcg gtgtcgtttg
     1981 tgacctcgtc gggtatggcc agggccagcc agtgttgcca gacatccggg ttcacttccg
     2041 cgctgctgcg ccaccgttgc gagaccaggg acaaaacgac ccagcgcccg cccggaaccg
     2101 gctggctgct ttgctcaaca ggcacgagtg agatgccggg gtcttccttg cgcacataac
     2161 ggtctagcgc ctcggtggcg tggacccgca ctacgcgctg cgcaaactga tggccgaagg
     2221 gatttcctgc ccggagtgac acggtatagg tgccaggttc ggcgtagata tgcctgggat
     2281 tttcctcggt actctcggtt ccatcgccga aacgccacag gtacgagaca tcgccggtgc
     2341 cttcatcgga ggcattggtg aattcaaatt cgtcaatagt gacgccccag ggaggactca
     2401 tatcaaaacg ggcgacggga ggtttgccgc gcacagtcaa aaaggcggcc tgcgtattgg
     2461 tgttggaggt cccctgtgca tcggccaccg tcaacgacac ggtcatgggt cctgcgactt
     2521 catagatatg ggtgggattc tggtcggtgc tggtctggcc gtcgccgaat tcccagaacc
     2581 aggaggcgat aggtgcatgt cccggttggg agatgtccga gaatgacacg gcggtcctta
     2641 gaaacggttg caggtcggag caggtaaagc cggcctcagg cggtattgcg ttgactgtaa
     2701 tatagttgtg tttgattgcc tcgtcctggc cgtgcggaga ggtcacggcc aaagaaaccg
     2761 tatacgagcc aggcgatgcg taggtatggg tttgcgcggc tccggctgcg gtgccgccgt
     2821 cgccaaatga ccactgccag gaggtgatgt tcgccgtccc gggcgctgat gcgtcggcaa
     2881 aaacgacggg cgaaccgaca ggcacaaccg tctccagtgc gctgaaatca gcgatgggcg
     2941 ccacccgggg acgcaccgtg atgaacttgt gtttggtgag ggttgcgctc tcgtccccgg
     3001 cctgtacggt gagggttatg tcgaaggaac ccgcggagtc atatgcatga gaaggcgccg
     3061 gggccgcgct ggtcgtcccg tcgccgaagt cccacgccca cgacgtaatg gttcctcctc
     3121 cggcgattcg ggagagatcc tgaaaaatga tcgcatcgcc ttcgtatggt tgcag
//
LOCUS       DAOPBQ010000449         3164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24355_length_3164_cov_3.172403, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000449 DAOPBQ010000000
VERSION     DAOPBQ010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24355_length_3164_cov_3.172403"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="PKW60_12665"
     CDS             complement(<1..1347)
                     /locus_tag="PKW60_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00331.1"
                     /translation="MRNVLFPKAPLVRVVVIVCVGCLSAQAELREPGSIAVLADFVEQ
                     FKHGTVTNDLTSSASYAEGALEDRMAAIFLHPTGLDDAILSFNGIPVPELQDGQRCIL
                     YFHIGFRPGIDWTSPSKAPNGVRFSVFVEGAAVFEEEVAGPGWNPRCVDLSSWSGQLV
                     SVEFHTNAISGNSSYDWAVWGRPLLLHFWPAQTPSTLPAQAEGLSLIEVACEQTVKVR
                     LADGRRPLTVMLEPGTHLLPHAFDDFSAPAVEIEPDAATVMGVSTGIFLPELEAAPPE
                     LSSPLVTVGQPFDIIQRIRNRGQGTHTGRATWSLRSGDGRTLAELGPGAMETPAVERL
                     GPGEEAVLVWRGLTAQSPGEWLLDTGRSLRVHVFAEARVPPHPRNGEPDVIVERNGDI
                     RAVVANDWSRLAVAVDESGAAYALADTWDGTAWQRVASLYPLCRLVLRHPDNSLEEL"
     gene            1722..>3164
                     /locus_tag="PKW60_12670"
     CDS             1722..>3164
                     /locus_tag="PKW60_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00332.1"
                     /translation="MDAEKRIQELESQVLRLTRELQQFRETRKPPGTDSEAPHPAPSH
                     SPSPETSSFTAPVATRAWPAWVSRLAVVFVTVAIILAAGKSASTSAFDLRSKALAAYS
                     MAALLLLLGAAFPDRRSLLASLALGTGLAAAYFMTYAAFFITNARLFAEPWYALPVLF
                     AAILGMTATVAWRRSYAAAVIAWPVVYYSFGLTASDASTMPQLTYALSGVALASAGAV
                     LLHIRFHWRFLVWLVLLGSYGTILPFLYSQPQGILLTPEAYFWISRGALAFVFVVLGL
                     ATVLDVRRPDQRSRGMLLLSLLNSAVFFPAVWTGIHAFHAPHAWVFRLAFTVVLATLA
                     LLAETRGSRKNYLFQAYIAQAFVMAILAMISLYPGITLWPALALVCFALAVFYQHSGA
                     IILKAGSILLLFVVAVGAFFTVQAPGYAVWGQHAIPARWLGCAGPAIVFFFIALYYER
                     TTRSWPMQERRRSGQWFLADTFLDPPGPSVA"
BASE COUNT          551 a         1019 c          940 g          654 t
ORIGIN      
        1 caattcttcc agggagttgt ccggatggcg caataccagc cggcagagcg gatagagcga
       61 cgccacgcgc tgccaggcag tgccatccca ggtgtcagcc agggcgtatg ccgcgccgct
      121 ttcgtccacc gcgaccgcga gccgcgacca atcgttcgcc acgacggcac ggatgtcgcc
      181 gttgcgttcg acgatcacgt cgggctctcc gttacgagga tgtggaggca cccgcgcctc
      241 tgcaaaaaca tgcacacgca ggctcctgcc ggtatccagc agccattctc ccggcgactg
      301 cgccgtaagg ccgcgccaga ccagcacggc ctcctcgccg ggcccgagcc gctctacggc
      361 gggcgtttcc attgcgccgg ggcccagttc cgccagggtt cgcccatctc ccgagcggag
      421 gctccaggtg gcccggccag tgtgagtgcc ctggcccctg ttgcgaatgc gctggatgat
      481 gtcaaagggc tgtccaactg tcaccagggg cgagctgagt tccggaggag cggcttccag
      541 ctcagggagg aagatcccgg tggagacgcc cattacggtc gcggcgtcag gttcaatttc
      601 cacagccggc gcggaaaaat catcaaacgc gtgcgggagg agatgggtgc ccggctccag
      661 catcacggtc agtggccggc gcccatcggc gaggcgaacc ttcactgtct gttcgcaggc
      721 tacctcaatc agactcaggc cttcggcctg agcaggcagg gtcgatggcg tctgtgcggg
      781 ccagaagtgc agaagcaggg gcctgcccca aacggcccag tcataactcg agtttcccga
      841 tatcgcattc gtgtggaatt cgacgctcac caattggccg gaccaggacg acaggtcgac
      901 gcagcgagga ttccagcccg gccccgccac ctcctcttca aaaacggccg ctccctccac
      961 aaatacggaa aagcgaaccc cgttgggcgc ttttgaaggg ctggtccagt cgattcccgg
     1021 ccgaaatccg atatgaaaat ataggatgca tcgctgaccg tcctgcagct cgggcacagg
     1081 aattccattg aagctcagga tggcatcgtc cagacccgtc ggatgcagaa aaatagccgc
     1141 catccggtct tctagcgcgc cctcggcata gctcgccgag gaggtcaaat cgttggtaac
     1201 cgtcccatgc ttgaattgtt ccacaaaatc ggccagcaca gcgatcgaac ccggttcacg
     1261 cagctcagcc tgagcggaaa gacatccaac acacacgatt accacgactc gaaccaaggg
     1321 cgctttggga aataagacgt tacgcatgtt ttctctctgt attaccacgc gttgaccagg
     1381 caaaagccga tccgatgctc aatcggcggg gtgccggacg agaggaaatg cactgggcga
     1441 aggcggatgc ctccgtgtgc agcctcaatc gtccaggccg gaacggaaaa tctgggcaaa
     1501 aacatagtct gggcaaaagt atagattgca cggatccaca ggtcaatttt ctgcttaaat
     1561 tacgctcctc aggggcagtc aggtccgcca tttccgatct cggcgcgttg tctactggaa
     1621 tgcccaaatt cctgccgata caataccctc aacaggggcc cgggcgcttg gggcgaggcc
     1681 ctcgagaccg ttcccttcga aacaacgaac cgagaacgcg tgtggacgcc gagaagcgca
     1741 tacaagaatt ggaatcgcag gtcttgcggc tcacccgcga gttgcagcag ttccgtgaaa
     1801 ctcgcaagcc gccaggcacg gattcagaag cgccgcatcc tgccccgtcc cactcccctt
     1861 cgcccgagac ttcttctttc accgcgcccg tagcgacgcg cgcctggccg gcgtgggtga
     1921 gccggctcgc ggtggttttt gtgactgtgg ccattatcct ggcggctggc aagagcgcca
     1981 gtacgtccgc cttcgacctt cgcagcaagg ccctggccgc gtacagcatg gcagctctcc
     2041 tgctgctcct gggggcggca ttccccgacc ggcgcagtct cctggccagt cttgccctcg
     2101 ggaccggcct cgcagccgcc tatttcatga cgtacgcggc gtttttcatc acaaatgccc
     2161 ggctgttcgc cgaaccctgg tacgcattgc ctgttttgtt cgcggctatc ctggggatga
     2221 ccgcgaccgt tgcctggcgc cgcagctacg cggccgccgt cattgcctgg ccggtcgtct
     2281 attattcgtt tggcctgaca gccagcgatg cgtccacaat gccccagctt acgtatgccc
     2341 tgagcggcgt ggctctggca tcggctggcg ccgtcttgct tcatatacga ttccactggc
     2401 ggtttctcgt gtggctcgtc ctgcttggtt cctacggcac gatccttccc ttcctgtatt
     2461 cccagcctca aggcatcctg cttacgccgg aagcctattt ctggatttcg cggggcgcgc
     2521 tggcgtttgt gtttgtggtt ctggggcttg ctacggtgct tgatgtgcgg cgcccggacc
     2581 agcggtcccg gggcatgctg ctgctgtccc tgctgaattc cgcggtgttt ttccccgcgg
     2641 tatggactgg gattcatgcc ttccatgcgc ctcacgcgtg ggtgtttcgc ctggccttca
     2701 cggtggtgct ggccacgctg gccctccttg ctgagacacg cggctcgaga aagaattacc
     2761 tgtttcaggc gtacattgcc caggcgttcg tgatggccat ccttgcgatg atctcgcttt
     2821 acccgggaat taccctgtgg cccgccctgg cgctcgtgtg ttttgcgctc gccgtgtttt
     2881 atcagcattc tggcgccatc atcctcaaag cgggcagcat cctgttgctg ttcgtcgtgg
     2941 cggtcggcgc gttcttcacg gttcaggcgc ccggatacgc ggtgtggggc cagcacgcca
     3001 tacccgcacg ctggctgggg tgtgcggggc cggccatcgt cttctttttt atcgccctct
     3061 attacgagcg gactacgcgc agctggccca tgcaggaacg ccgccggagt ggccagtggt
     3121 ttctggccga tacctttctt gacccgcctg gcccctccgt ggcc
//
LOCUS       DAOPBQ010000450         3160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24401_length_3160_cov_5.434461, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000450 DAOPBQ010000000
VERSION     DAOPBQ010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24401_length_3160_cov_5.434461"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /locus_tag="PKW60_12675"
     CDS             <1..1737
                     /locus_tag="PKW60_12675"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPK00333.1"
                     /translation="DVPGVDWTLYFTNTGSADSPVLEAIRSLDVVIPDVARPVLHQLE
                     GSVAGPTDWRPFSQDLVPGQTVAIQPQSGRSSKGASPFLTCAWPGGGVVTAIGWTGRW
                     TASVEYGGQALRLQAGLEKFHAKLRPGETVRGPRILQVYWQGDDVWYGYNAFRQVMLR
                     HIMPKVDGHPVVPPIAHLSTAFYEMDDGTEADVMAHLESAKGLGFEYFWLDAYRGITR
                     FPVIGHYVFPVEREVDKNRFPNGLAPISKAAHDAGMKFLLWFEPERISPGSLMDQEHP
                     DWVVMPPPERMGYGGMLNLAIPEARQYATDYLNAAIDAYGIECLRIDNAVDYGVLWGI
                     LDERDPDRAGIDEVRYVEGLYRMWDDILAAHPGLFIDNCASGGQRIDLELSARSIPLW
                     RTDGTIMPLLEKDYEESALRNQVITAGLNRYLPFHTSGQMGVTPYLFRSGFNGGVAFA
                     EDIRAADYPRDQLAAAIAEGKRLRKYSFGDFYVLTSVSTDPTVWLVMQYHLAGSGEGM
                     VLAFRRPASRYTSFACPLRGIAPEANYEVILSRGYVPEAPVRMTGAEFASFEARIEDA
                     PGSLLMEYRAVE"
     gene            complement(1895..>3160)
                     /locus_tag="PKW60_12680"
     CDS             complement(1895..>3160)
                     /locus_tag="PKW60_12680"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPK00334.1"
                     /translation="ILAETQHDIIKRAALMGLGRFGDETVVEPIVAAASGGAPLVQAT
                     VVPALEQLRGRAATRRILAAYPDLESDTQLRMIRMFGRKNDPAYLPLLNEAALSPDAA
                     FRRAALDALASARSVDALPILVAVAREGTEEERALALKAASNVAGSIGGAGDAEAAGN
                     AFLELYTLAGDAMLQRTALEGLARYPVAAAHDAVVAALADEALASTARAALPGLFGAL
                     VASGARDEALEIFDRVLRDGASPEVLVGMAAHLQGLQTTLDTTQLLGVIKQWHVIGPF
                     QWKNDDDWLTAFVDEPSINLDGVCMDGDAERRWTPVTSRDALGLVDLVGTIGQHDRVF
                     AYAYTEIDVPEASPAQIRIGSDDGNAVWLNGEKIWENRVDRGAAPDQDIAPCQLIQGK
                     NRILVKISQGAGGWNFMLRITRPDGTAPW"
BASE COUNT          548 a          929 c         1081 g          602 t
ORIGIN      
        1 gacgtgccgg gggtggactg gacgctatat ttcacgaata cgggttcggc cgattccccg
       61 gtgctcgaag cgattcgctc gctggatgtg gtgattccgg acgttgcgag accggtgctg
      121 caccaactcg aaggaagcgt cgcgggcccg accgactggc ggccattttc gcaggacctc
      181 gtgcccgggc aaacggtggc gattcaaccg cagtcgggtc gttcttcgaa aggcgcctcg
      241 ccgtttttga cgtgcgcgtg gccgggtgga ggcgtagtta cggcgatagg atggacgggg
      301 cgctggacag cttcagtcga atatggcggc caggcgcttc gccttcaggc gggcctcgag
      361 aaattccatg cgaaactgag acccggtgag acggttcgcg gaccgcgcat cctgcaggtg
      421 tactggcagg gcgacgacgt ctggtacggc tacaacgcat tccggcaggt gatgctgcgc
      481 cacatcatgc cgaaggtcga cgggcatccc gtggtgccgc cgattgcgca cttgagcacc
      541 gcgttctacg agatggacga cggcaccgag gccgatgtga tggcgcatct cgaatccgcc
      601 aaaggcctgg gttttgagta tttctggctg gacgcgtacc gcggcatcac ccgctttccg
      661 gtcatcgggc attacgtatt tcctgtcgag cgcgaagtcg acaaaaaccg ttttccaaat
      721 gggctggcgc cgatttcgaa ggcagctcac gacgccggca tgaagtttct gttgtggttt
      781 gagccggagc gcatatctcc gggctccttg atggaccagg aacatcccga ttgggtggtg
      841 atgccacctc cggaacgcat gggatatggc ggcatgctca atctggccat ccccgaggcc
      901 cgacagtacg cgacggatta cctgaacgcc gccatcgacg cttacggcat cgagtgcctg
      961 cgtatcgaca atgcggtgga ttacggcgtg ctgtggggaa tcctcgatga gcgcgacccg
     1021 gaccgggcgg gcatcgacga agtacggtac gtcgagggcc tctatcggat gtgggacgat
     1081 atcctggcgg cgcatcccgg cctgttcatc gacaactgcg cgtcgggcgg gcagcgcatc
     1141 gatctcgaat tgagtgcgcg atccatcccc ctgtggcgga ccgacggcac gatcatgccg
     1201 ctgttggaga aggattacga ggaatcggcg ctgcgcaacc aggtcatcac cgccgggctg
     1261 aaccggtatc ttccgttcca caccagtggc cagatgggcg tgacgccgta cctgttccgt
     1321 agtggattta acgggggtgt ggcgttcgcc gaagatatcc gcgcggcgga ttatccgcgt
     1381 gatcagctgg ccgcggccat tgccgaggga aaacggctgc ggaaatacag tttcggcgac
     1441 ttctatgtgt taacgagcgt ctcaaccgat ccgacggtgt ggctggtgat gcagtatcat
     1501 cttgccggct ccggcgaggg gatggtgctg gcgttccggc ggcccgcgtc acgctacacc
     1561 agttttgcat gccctctccg ggggatagct cccgaggcca actatgaagt cattctgtcg
     1621 cgcgggtatg tgcccgaagc gcccgtccgg atgacaggcg ccgagttcgc cagtttcgaa
     1681 gcgcgtatcg aagacgcccc ggggtcactg ctgatggagt accgggcagt ggaatgaact
     1741 tcggcgcccg tgttgcgctg gcgttgcacg atttccttac agacgttcac gcacgcgcag
     1801 actctgctgt ccgcggtgtc cccccgaaga gttcccaatg cttaatgcgt tcgaaaaaag
     1861 ctccggggca gaggatacgc ctggcctgcc gggtttacca cggtgcggtg ccgtcgggcc
     1921 gggtgatgcg gagcatgaag ttccaaccgc cggcgccctg gctgatcttg acgaggatgc
     1981 ggtttttgcc ctggatgagt tgacaggggg cgatgtcctg gtcgggcgcg gcgccgcggt
     2041 ccacgcggtt ctcccagatt ttctcgccgt tcaaccagac cgcgttgccg tcatcggagc
     2101 cgatgcgaat ttgcgccgga gatgcctccg gaacgtcaat ttctgtgtag gcatacgcaa
     2161 aaacgcggtc atgttgaccg atagtgccca ccagatcgac cagaccgagg gcatctcggg
     2221 acgtgacggg agtccagcgc cgctcagcgt cgccgtccat gcacacgccg tcgaggttga
     2281 tgctgggttc atcgacgaat gcggtgagcc agtcgtcgtc gtttttccat tgaaaggggc
     2341 cgatcacgtg ccattgcttg atgacgccga ggagttgggt cgtgtcgagc gtcgtctgaa
     2401 ggccctgcaa atgcgccgcc atgcccacga ggacttcggg ggaggcccca tcgcggagca
     2461 cgcggtcgaa gatttcgagc gcctcatctc gtgcgccgga tgcgaccagg gcgccgaaga
     2521 gtcccgggag agcagcccgt gccgtgctgg ccagggcctc gtcggcaagc gcggcgacga
     2581 cggcgtcatg cgcagccgcg acaggatagc gggccagccc ttccagagcg gtgcgttgca
     2641 gcatggcgtc gccggcaagg gtgtaaagct ccaggaaggc gtttcctgcg gcttcggcat
     2701 cgccagcacc tccaatgctg cccgcgacgt tggaagcggc cttaagggcg agggcgcgct
     2761 cttcctccgt accttcgcgt gcgacggcca cgagtatggg cagggcgtcg accgagcgcg
     2821 cggaggccag cgcatcgagc gcggcccggc ggaaggcggc atccgggctc agggcggctt
     2881 cgttgagtag tgggagatac gccgggtcgt tcttgcggcc aaacatccga atcatgcgga
     2941 gttgcgtgtc ggactccagg tcaggatacg cggcgaggat gcgtcgcgtg gcggcgcggc
     3001 cgcgaagttg ttcgagcgcg gggacgacag tcgcctgcac taggggagcg cctcccgacg
     3061 cggcggcaac gatgggttca accaccgtct cgtcgccgaa tcggcccagg cccatcaggg
     3121 ccgcgcgttt gatgatgtcg tgctgggttt cggcgaggat
//
LOCUS       DAOPBQ010000451         3154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24459_length_3154_cov_4.167151, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000451 DAOPBQ010000000
VERSION     DAOPBQ010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24459_length_3154_cov_4.167151"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKW60_12685"
     CDS             <1..272
                     /locus_tag="PKW60_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00335.1"
                     /translation="WQCHLEHAPWHPRQYHDVAVFDGRLWVLEGWNQENRNDVWHSAD
                     GVEWQELPGTPWAPRHASSLFVYKDALWVVAGNNMQSDVWKLERR"
     gene            complement(302..1594)
                     /locus_tag="PKW60_12690"
     CDS             complement(302..1594)
                     /locus_tag="PKW60_12690"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00336.1"
                     /translation="MNKSSDMNRRRFLKTAGTAAAVAATGPAIVGAAEPTSDPVRIAH
                     IGTGVRGWDLIKYTGPHKGAKVVMVCDVYKPHLERGVEASNNPDVKAVTDYHDVLADP
                     QVEAVIIATPDHWHEQMLIDAVNAGKDVYCEKGWTISIDAAKRMRAAVKQQGRVMQLG
                     HQGRQWPAATAGGDLIRADRIGPVTFVKVGRYFNGTPERAPWRWFGNYSWYDRPDPAQ
                     VRADVDWEKWLGPAPAIDFNERHFWHWRCYWPYGTGQAGDLLSHEMDFVQTVLGWGIP
                     DTCVCAGLNAFWKDDREVPDTWLATYQFESQNCSASFEGSMNSDRRQTPEFIGRDGRL
                     IYNGIGQDANRYEVYPDAPAWRMGDTPIEPVERFDPAAAPSQPTHMEDFFEAVRTRKP
                     PLCNEDEAFIEAATLLMSVESYKQKRQVRWDKAREEIV"
     gene            complement(1645..2547)
                     /locus_tag="PKW60_12695"
     CDS             complement(1645..2547)
                     /locus_tag="PKW60_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPK00337.1"
                     /translation="MRMGWVLLLMAGVCAAQTAEEPAAGIAAPGAVVEKLADGFEFTE
                     GPASDAAGNVYFTDQPNDRIMKWSIGGELTTYMQPCGRSNGLCFDRDGYLWACADEKN
                     QLWRISPGKDVTVLIEACEDKLLNGPNDLWIRPDGGAYLTDPFYKRNYWDRSETMEQP
                     CQGVYYLAPDRKTLTRVLDDLEQPNGIIGTPDGKQLYVTDIKAGKTYAYTMAADGRLT
                     EKTLFCEMGSDGMTIDNEGNVYLTGKGVFVFDKSGRPIDHIVIDEPWTSNVCFSGRTM
                     NDLFITASKAIYRLRTRVKGVGSQ"
BASE COUNT          548 a         1041 c          900 g          665 t
ORIGIN      
        1 actggcaatg ccatctcgaa cacgcgccct ggcatccgcg ccagtaccac gacgtggccg
       61 tgtttgacgg gcgcctctgg gtcctcgaag gctggaacca ggagaaccgc aacgacgtct
      121 ggcattcggc ggacggggtg gagtggcagg aactccccgg cactccctgg gccccccggc
      181 acgcctcaag cctgttcgtc tacaaagacg cgctctgggt cgtcgccggc aacaacatgc
      241 agagcgacgt ctggaagctc gaacggcggt aagcggcccg ccttatccgg gatcatcccc
      301 gctacacaat ttcctcgcgc gctttgtccc agcgtacctg gcgcttctgc ttatacgact
      361 cgacgctcat cagcagtgtg gccgcctcga tgaaggcctc gtcttcgttg cacagcggcg
      421 gtttccgcgt gcgcaccgcc tcgaagaagt cttccatgtg cgtcggctgg gacggtgccg
      481 cggccggatc gaagcgctcc acaggttcga tgggtgtatc gcccatccgc cacgcgggcg
      541 cgtccggata cacctcgtag cggttcgcat cctgcccgat gccattgtaa atgagccggc
      601 catcccgtcc gatgaactcg ggggtctgcc tccggtccga gttcatgctg ccttcgaaac
      661 tggccgaaca attctggctt tcgaactggt aggtggcgag ccacgtgtcg ggcacttcac
      721 ggtcgtcttt ccagaacgca ttcagtcccg cgcagacgca ggtgtcggga atgccccagc
      781 ccagcaccgt ctggacgaaa tccatctcgt gcgagaggag gtcgcccgcc tggcccgtgc
      841 catagggcca gtagcagcgc cagtgccaga aatgccgttc gttgaaatcg atcgccggcg
      901 cgggccccag ccatttttcc cagtccacgt ccgcgcgcac ctgcgccggg tcgggccggt
      961 cataccacga atagttgccg aaccagcgcc acggcgcccg ctcgggcgtg ccgttgaagt
     1021 agcggcctac cttcacgaac gtgaccgggc caattcggtc cgcgcggatc aggtcgccgc
     1081 ccgcggttgc agcgggccat tggcggccct ggtggccgag ctgcataact cgtccctgct
     1141 gttttaccgc cgcgcgcatt cgtttcgcgg cgtcgatcga gatggtccag cccttttcgc
     1201 agtacacgtc cttccccgcg ttcacggcat cgattaacat ctgctcatgc cagtgatccg
     1261 gcgttgcgat gatcacggcc tccacctgcg gatcggccag gacatcgtgg taatccgtga
     1321 cggctttcac gtcgggattg ttgctggcct ccacgccgcg ctccaggtgc ggtttgtata
     1381 catcgcagac catcaccact ttcgcgcctt tgtgcggccc cgtgtatttg atgaggtccc
     1441 atccgcgcac gcccgtgccg atgtgcgcga tccgaaccgg atcactcgtt ggttccgccg
     1501 cccccacaat cgccggtccc gttgccgcca cggccgcggc cgtgcccgct gtcttcaaaa
     1561 aacgccgccg attcatatca ctgctcttgt tcatacccac ctcgtctgtc cggttgtttc
     1621 acgaaaactg gttgagcttc ttgcttactg gcttcccacg cctttcacgc gcgtgcgcag
     1681 ccggtagatc gccttactgg ccgtgatgaa caggtcattc atcgtccgtc cgctgaagca
     1741 gacgttcgag gtccacggct cgtcaatgac aatatgatcg atcggcctgc ccgacttgtc
     1801 aaaaacaaaa acgcccttcc ccgtcaaata cacattcccc tcgttgtcga tggtcatgcc
     1861 gtcggaaccc atctcgcaga agagcgtctt ttcggtcaac cggccatcgg ccgccatggt
     1921 gtaggcatag gtcttcccgg ccttgatgtc cgtcacgtag agctgcttcc cgtcgggcgt
     1981 gccgatgatg ccattgggtt gctcgaggtc atcgagaacg cgcgtcaggg ttttgcgatc
     2041 aggcgccaga taatatacgc cctggcaggg ctgttccatg gtctcgctgc ggtcccagta
     2101 attgcgcttg tagaacgggt cagtgaggta ggcgcctcca tccggccgga tccacaggtc
     2161 gttcgggccg ttgagcagct tgtcttcgca ggcttcaata agcacggtca cgtctttgcc
     2221 ggggctgatg cgccaaagct gatttttttc gtccgcacag gcccacagat agccgtcgcg
     2281 atcaaaacag aggccattcg accggccgca cggctgcatg taggtggtca gttcgccacc
     2341 gatgctccat ttcatgattc gatcgttggg ttgatcggta aaatagacgt ttcccgcggc
     2401 gtccgaggcc ggcccttccg taaactcaaa cccgtcggcg agtttctcga ccacggcccc
     2461 cggcgccgca atgcccgcgg ccggctcctc cgcagtctgt gcggcgcata ccccggccat
     2521 cagcaatagc acccatccca ttcgcatgac cctgctcctt gcacggcatc ccgccgcagt
     2581 cccgcctcta tgccggtgac tatagcacag cctctgttcc agccggatta ccgccggcct
     2641 tccgcgcccg ctggattttg taccagaagc ccgtcaaacc agttgcgggt cttactgatc
     2701 gtgccgttac gccatatgtt ttcttgccgg atatgagacg cctatctata tcaaccggcg
     2761 gctcgcgttc ctcgtaacac tcgctcccca agagcacagc aggaagaatc catactggag
     2821 cagcgtatcc ccttcccgcg gggagaggat ggcaaggctc taaccccagg gtgaacttct
     2881 cattcctgac aagaaagcaa atggtgtgag gatattctgc cggccagatt agcaaatgtc
     2941 ttgacagggc ggatcgaaaa ggcactggcg ccttgtcctg ggcttacaaa agatcgcgat
     3001 cacgctccgc gccgggggtc actgccgcag gctgcttttt cctgtgggcg gcatctgcca
     3061 taatcgagtc tgtcacaccg gtctggagga cgatgtatga cgctgcggga acgttttatc
     3121 gccgcattgg agcgccgccc tgtcccgggg cggg
//
LOCUS       DAOPBQ010000452         3152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24480_length_3152_cov_3.939619, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000452 DAOPBQ010000000
VERSION     DAOPBQ010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24480_length_3152_cov_3.939619"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..1606
                     /gene="fumC"
                     /locus_tag="PKW60_12700"
     CDS             215..1606
                     /gene="fumC"
                     /locus_tag="PKW60_12700"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HPK00338.1"
                     /translation="MDFRIETDSMGEMRVPAGMYYGCQTARSIENFKIGIETMPREVI
                     RAFGILKKAAALTNYELGLLGPDLRSAICQAAGEVMDGHLDEHFPLVVWQTGSGTQSN
                     MNVNEVISNRAIEILGGELGSKNPVHPNDHVNMSQSSNDTFPTAMNIAAAEQVSHHLL
                     PALETLRDTLQAKSEAFGEIIKIGRTHLMDATPLTLGQEFSGYAQQLTNGIERITAAL
                     PGLFELALGGTAVGTGLNTKKGYDEKVAAKIAELTGLPFVTAPNKFEALAAHDAVVMM
                     SGALKTVACSLMKIANDIRWLASGPRCGLGELRIPANEPGSSIMPGKVNPTQCEAVTM
                     VAAQVLGNDTTINVAGASGNFELNVYKPVLIYNLLQSIRLLGDAARSFNDHCAVGIEA
                     DEKRIDALLHESLMLVTALNRTLGYDNAARIAKAAYANGTTLKEEAVQLGLLTAEEFD
                     EAVDPAKMIGPSD"
     gene            complement(1627..2619)
                     /locus_tag="PKW60_12705"
     CDS             complement(1627..2619)
                     /locus_tag="PKW60_12705"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00339.1"
                     /translation="MARKLRIGFIAAGGIAPGHWDRIHETKKATVVALTDPSKKSLAA
                     FYQRCPGSEKLPAYCDYRDMLAHEELDGVVVQSPHTVHYEQTVASLKKGLHVLSEKPL
                     TCTVEHAKRLIALAQQVNRVLMISYQRHYFPEFRYIRAQIAKGAIGEVQFVQAVQAQE
                     WLRAVKGTWRQKLAESGGGQINDSGSHLIDIIMWLTGLKIDEVYAEMENFTTEVDINS
                     TLTLKFGNGALGSVAIIGNAPAFHEDITVVGSKGAFYLRHGQPLVHQDAMGKAIKVVL
                     PRYTKNPDANFIDAILGKDEPQTPPICGLRVIEVTEAAWKSARSGRVVKVKTSD"
     gene            2856..3044
                     /locus_tag="PKW60_12710"
     CDS             2856..3044
                     /locus_tag="PKW60_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00340.1"
                     /translation="MREVLKAIELIGGGGGVMAMVCGGAALVLGFTNAGAAGIKYGAV
                     AFIIGIAVKLFRRLILPD"
BASE COUNT          611 a          925 c         1020 g          596 t
ORIGIN      
        1 acgagaggcc ggcgacgacc gtgtaggcga cattttgaag ggcatcacag ccctgaccgc
       61 ggccatgaag gcggtggccg gataggctcg catgaatttg ttcgcagtgt ggcacgggcg
      121 ccggcgcgtg tgcggcaggc gcccggctgc ctgcgcttcc cggcggcata cgggtggcag
      181 agggagagcc tgaacctttc cgaaggagac aacggtggat ttccggatcg aaacggactc
      241 gatgggtgaa atgcgcgtgc cggccggcat gtattacggt tgccagaccg cgcgttcgat
      301 tgaaaacttc aagatcggca tcgagaccat gccgcgcgag gtcattcgcg ccttcgggat
      361 cctcaagaag gccgcggcgc tgaccaacta cgaactgggc ctgctcggac ccgatctgcg
      421 cagcgccatc tgccaggcgg ccggcgaggt catggacggc catctggacg aacatttccc
      481 ccttgtggtg tggcaaacgg gcagcgggac ccagagcaac atgaatgtga acgaggtcat
      541 ctccaaccgg gcgatcgaaa tcctcggggg agagctgggc agcaagaacc ccgtgcatcc
      601 caatgaccac gtgaacatgt cgcaatcgtc aaacgacacg ttcccgaccg ccatgaacat
      661 cgcggccgcg gagcaggtgt cgcatcacct cctgcccgcg ctggagacgc tgcgcgacac
      721 gcttcaggcg aaatccgagg ctttcggaga gatcatcaag atcgggcgga cgcatctgat
      781 ggacgccacg ccgcttaccc tgggccagga attctcgggg tacgcccagc agctcaccaa
      841 cggcatcgag cggatcacgg ccgctttgcc ggggttgttt gaattggcgc tgggcggaac
      901 ggcggtcggc acgggtctga acacgaagaa aggctacgac gagaaagtgg ccgcgaaaat
      961 agccgagcta acggggctgc cgttcgttac tgcgcccaac aagttcgagg cgctggcggc
     1021 ccatgacgcc gtggtgatga tgtccggcgc actgaagacc gtcgcgtgca gcttgatgaa
     1081 gatcgccaac gacattcgct ggctggccag cgggccgcgg tgcggcctgg gcgagctgcg
     1141 gattcccgcc aacgagccgg gctcgtcgat catgcccgga aaggtgaacc cgacccagtg
     1201 cgaagcggtg accatggtgg ccgcgcaggt gctcggcaac gatacgacga tcaacgtggc
     1261 gggcgcatcg ggcaattttg aactgaacgt gtataaaccc gtgctgatct acaatctgct
     1321 gcagtcgatc cggcttctgg gcgacgccgc gcgctcgttc aacgatcact gcgcggtggg
     1381 catcgaagcc gacgagaaac ggatcgatgc gctgcttcat gaatcgctga tgctggtcac
     1441 ggcgctcaac cgcacgctgg gttacgacaa cgccgcccgg atcgccaagg ccgcctacgc
     1501 caacggcacg accctcaaag aggaagcggt ccagctcggc ctgctcacgg cggaggaatt
     1561 cgacgaggcc gtcgaccccg cgaaaatgat cggtcccagc gactgaggcc ggggtagctt
     1621 ccgccttcag tcgctcgtct tcaccttgac gacccggccg ctccgggcgg atttccacgc
     1681 ggcttcggtg acttcaatga cacgcaaacc gcagatcggc ggcgtttgcg gctcgtcttt
     1741 gccgagaatg gcgtcgatga agttggcgtc gggatttttc gtataccgtg gcagcaccac
     1801 cttgatcgct ttgcccatcg cgtcctggtg gacgagaggc tgcccgtgac gaaggtagaa
     1861 cgcgcctttg gagcccacca cagtgatatc ctcgtgaaat gcgggcgcgt tgccaatgat
     1921 ggccacgctg cccagggcgc cgttgccgaa cttcagggtg agggtcgaat tgatgtcgac
     1981 ctcggtcgtg aaattttcca tttccgcgta gacttcatcg attttgaggc cagtcagcca
     2041 catgatgatg tcgatcagat ggctgccgga atcgttgatc tggccgccgc ccgattcggc
     2101 cagtttctgc cgccacgtgc ccttgaccgc ccggagccat tcctgcgcct gtacggcctg
     2161 cacgaactgg acctcgccga tggcgccctt ggcgatctgc gcccggatgt agcggaattc
     2221 aggaaaataa tggcgctgat aggagatcat cagcacccgg ttcacctgtt gagcgagagc
     2281 gataaggcgt ttggcatgct ctaccgtgca ggtcagcggt ttttcggaaa gcacgtgcag
     2341 gccttttttg agcgatgcca cggtctgttc gtaatgcacc gtgtgcgggc tctggaccac
     2401 cacgccgtcg agttcttcat gagccagcat atcccggtaa tcgcagtagg cgggcagctt
     2461 ttccgacccc ggacagcgct gatagaaggc ggcaagcgat ttcttcgacg gatccgtcag
     2521 ggcgacgacc gtggccttct tggtctcgtg gatgcggtcc caatgtccgg gcgcgattcc
     2581 cccggcggcg atgaatccaa tacggagctt tctggccatg gtgcgcgcct cctctacagt
     2641 ctgttttcgc cagcacagcg gcatggtaac aacggtttcg gggcaaatca acgacagaag
     2701 gtgtaacgca tggagatgcg cccaaaaggc cggactggcg gcttcaaccg gcgccagccc
     2761 ggcattcttt cgaggctttg agatctctgc gctgtgagga ctggttctgt tatagtggat
     2821 gccgcaatgc gtatgcagaa gccgaggaac aggtcatgcg cgaggtgttg aaggcgattg
     2881 aactgattgg tggcggaggc ggcgtaatgg ccatggtctg cggtggcgcg gcccttgtgc
     2941 tgggcttcac gaacgcaggc gcggccggta tcaagtacgg cgcggtcgca ttcatcattg
     3001 gaatcgcggt caagcttttc cgccggctga ttctcccgga ttgacacaat ccggctgcgc
     3061 gggaggaagc gcttcatgtg gacgaagttc aaaggcctgt ttgtgccgtc ccccgaaagc
     3121 caaagggcgt ggcgggtgtt cctgcgcatc ga
//
LOCUS       DAOPBQ010000453         3151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24490_length_3151_cov_3.721253, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000453 DAOPBQ010000000
VERSION     DAOPBQ010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24490_length_3151_cov_3.721253"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PKW60_12715"
     CDS             <1..760
                     /locus_tag="PKW60_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HPK00341.1"
                     /translation="GFLEGIKVAKAAEGIPNYDVNKMATRQEMRAFVCGQCHVEYYFK
                     GEEKRLTYPWFNGYKADDVLAYYDSIGFEDWTHADTGAPMLKAQHPEFETWNQGIHSR
                     AGVACADCHMPYVRSGAMKISDHHVRSPLLNINNACQTCHKVSEDELKARAENIQAKT
                     MEMRDHSMGALVELIGAIQEAQSAGVPAERLDEARQAQRRASFLIDWVEAENSTGFHA
                     SQETARLMFISMDHIRKGQIALAGRSEQAASVMQ"
     gene            complement(851..2785)
                     /locus_tag="PKW60_12720"
     CDS             complement(851..2785)
                     /locus_tag="PKW60_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPK00342.1"
                     /translation="MGSDCISQPLLGIQNVTKSYGNQVLLRNVSLTIHENDRVGIIGR
                     NGCGKTTLIRLMAGLETPDAGTVTRRQGVRTALLQQETPSQPSETVGDVFEAATADIR
                     SLLEEHHTLAGELAAADSGPRRDRLEAEFEQAQHALRVREGWNIEAEVGRMATALRVP
                     GLDRSLSTLSGGELRRVYLAATLVRQPDVLFLDEPTNHIDAESARWIEDFLASYTGSC
                     VLITHDRYFLDRVVTRIVELEKQQLLCIGGNYESFLQQKTLREEHAARSEANRQGALR
                     RELAWLLRGARARSTKQKARIKRFDELEAREACKPQEALSFEILSPNRLGKRILEAEG
                     VSLTLDGRELFRDFSLAMQNGMRVGLVGANGCGKTSLLRVLMGQLEPDAGRIFIGEST
                     EFLYVDQSHREVNPAHTVLQFISNGVRDVEINGRRIHIPAYLERFLFDRSVMNMEMQY
                     LSGGERSRLDLARKLMAGGNFLVLDEPANDLDLPTLRVLEEMVAGFEGCALVVSHDRY
                     FLNRICTHLVAFEEGPNLVKVAGNYDDYLRYRKETAEAASDGSAAPPPKKRRPRESTA
                     PRRLTWHEKRELETIEEAISQAEAEISRLQNLVNEPSFYKQQNEQVQPVLTALQAAEK
                     RADELYERWSELDSIANAAP"
BASE COUNT          614 a          993 c          946 g          598 t
ORIGIN      
        1 gggctttctt gaagggatca aggtggccaa ggccgcggaa ggcatcccca actacgacgt
       61 gaacaaaatg gctacccgcc aggaaatgcg cgcctttgtg tgcggccagt gccacgtcga
      121 atactatttc aaaggcgagg aaaagcgcct gacctatccc tggttcaatg ggtacaaagc
      181 tgacgacgtg ctggcctatt atgattccat cgggtttgag gactggaccc atgccgacac
      241 cggcgccccc atgttgaagg cgcagcatcc cgaattcgag acgtggaacc agggcattca
      301 ctcacgggca ggcgtggcct gtgcggattg ccacatgccc tacgtccgtt caggcgccat
      361 gaagatcagc gatcaccacg tgcgcagccc cctgctgaac atcaacaatg cctgccagac
      421 ctgccacaag gtgtcagagg atgaattgaa agcccgcgcg gaaaacatcc aggccaaaac
      481 catggagatg cgcgaccaca gcatgggcgc cctcgtggaa ttgatcggcg ccatccagga
      541 ggctcagagc gcgggcgtgc cggcggaacg cctggacgag gcccgtcagg cgcagcgccg
      601 ggcgtcgttc ctgatcgact gggtagaggc cgagaactcc actggatttc atgcctccca
      661 ggaaaccgcc cgtctcatgt ttatctcgat ggaccacatc cgcaaagggc agatcgccct
      721 ggcgggccgg agcgagcagg ccgcaagcgt gatgcaatag cctcagcccg gtttttgcgt
      781 gacggcccag ggttgccggc tgcacaaggc ggccggatcg ccggatgagc gcaggcagac
      841 gattgcgccg tcatggggct gcgttcgcaa tcgaatccag ctccgaccag cgctcataca
      901 actcgtccgc gcgtttttcc gccgcctgca acgcggtgag caccggctgc acttgctcgt
      961 tctgttgctt atagaaggac ggctcattca ccaggttctg cagccgggag atctccgcct
     1021 ccgcctggct gatggcctct tcgatggttt ccagttcccg cttttcgtgc caggtgaggc
     1081 ggcgcggcgc cgtactctcg cggggccttc gttttttggg tggcggcgcg gctgagccgt
     1141 ccgatgcggc ctcggcggtt tctttccggt agcgcagata atcgtcgtaa ttgcccgcta
     1201 ctttgaccag attcggccct tcctcgaagg cgaccagatg ggtacagatg cgattcagaa
     1261 aataccggtc gtggctgacc accagggcgc agccttcaaa gccggctacc atttcttcaa
     1321 gcacgcgcag ggtcggcaga tcgaggtcat tggccggttc atcgagcacg aggaaattgc
     1381 cgcccgccat gagctttcga gccagatcca ggcggctcct ctctccccca ctcaggtatt
     1441 gcatctccat gttcataacg ctgcggtcga acaaaaaacg ctcgagatat gcggggatat
     1501 ggatgcgccg cccattgatc tccacatcgc gcacaccgtt cgagatgaac tgaagcacgg
     1561 tgtgggcggg gttcacttcc cggtggcttt gatcgacata caggaattcg gtcgactctc
     1621 cgatgaaaat ccggcccgca tcgggttcga gctgccccat gagcacgcga aggagcgagg
     1681 ttttcccgca tccgttggcg cccacgagac cgacgcgcat cccgttctgc atggccagcg
     1741 agaaatcgcg gaaaagctca cggccgtcca gggtcagcga cacgccttcc gcctccagta
     1801 tgcgttttcc aagccggttg ggcgacagga tctcaaagga taaggcctct tgcggcttgc
     1861 aggcttcgcg ggcctccagc tcatcgaagc gtttgatgcg ggccttctgc tttgtgcttc
     1921 gggccctggc gccccgcagc agccaggcca gttcgcgccg caatgcgccc tgccggttgg
     1981 cttcactccg cgccgcgtgc tcttctctga gtgtcttctg ttgcagaaaa gactcatagt
     2041 tcccgccaat gcacagaagc tgctgtttct caagctcaac gatccgggtg acaacgcgat
     2101 cgagaaaata gcggtcatgc gtgatgagga cacagcttcc cgtgtatgaa gcgaggaaat
     2161 cttcgatcca ccgggcgctt tcggcgtcaa tgtggttggt gggttcatcg agaaagagca
     2221 cgtccggctg tctgacgagc gtggcggcca gatacacccg gcgaagctct cccccgctca
     2281 gcgtcgacag agagcggtcc aggccgggca cccggagcgc ggtggccatg cggccgacct
     2341 cggcctcgat attccaccct tcccgaaccc gcaacgcgtg ctgagcctgc tcaaattcag
     2401 cctccagcct gtcccggcgc ggccccgaat cggccgccgc cagctcaccc gcaagcgtat
     2461 ggtgctcctc taacagggag cggatgtccg cagtcgctgc ctcaaaaacg tccccgacgg
     2521 tctcgctggg ctggcttgga gtctcctgct gcaagagggc cgttcggacg ccctggcggc
     2581 gcgtaaccgt ccccgcatcg ggagtctcca ggccggccat cagcctgatg agggtggttt
     2641 ttccacagcc gttccggccg atgatgccta cccggtcatt ctcgtgaatc gtgagcgaca
     2701 cgttccggag aaggacctgg ttgccatagc tcttggtgac attctgaatg cccagcagcg
     2761 gctgagaaat gcaatcggaa cccattactg cgttagaaat tgctccctgc gctgtgcaga
     2821 aacgaacacc tcttcgaatc cgcccggtgt ggatggtcga aaatcagaaa tctggcgttt
     2881 gtgagtgcgt ttgaacaaaa gccggcccaa tgcgcgcatc cacattataa cctaaagcga
     2941 aaacgatgaa aacgtgagcg cgtaggggca gacccctgtg tctgcccgca caatcccacc
     3001 tcggccgctt cgcggccacc cccggccgcg ggggacataa gaacccgaac cggagagatt
     3061 gagatgcggc gcacctgtcc cccgcgggcg gggctcagcc gtgtgccgac gaggacgtcg
     3121 gcgcacccag gggacctgtc ccccgcgggc g
//
LOCUS       DAOPBQ010000454         3150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24500_length_3150_cov_4.789984, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000454 DAOPBQ010000000
VERSION     DAOPBQ010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24500_length_3150_cov_4.789984"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PKW60_12725"
     CDS             complement(<1..461)
                     /locus_tag="PKW60_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00343.1"
                     /translation="MDDNQDQGLMKNQMLGIILMTVLVVIWLNYFMQRPAVGPEPAAP
                     EVQTAPGASLPEHSEDGAAPADGTDAASAWPELPPVPESVLPEDEVTIENGQLRLVFT
                     RIGARLKRAEVILDKDGNDVVELVPRSDRPDTDTIYPLGLWFAAQQELGDRL"
     gene            complement(555..830)
                     /gene="yidD"
                     /locus_tag="PKW60_12730"
     CDS             complement(555..830)
                     /gene="yidD"
                     /locus_tag="PKW60_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPK00344.1"
                     /translation="MRRVNTRYDSFSEREGCWVAKVLVVLIRGYQYGISPLLSAILGP
                     SCRFTPSCSQYAIEALERYGVIRGVGYAIRRIARCHPCSRGGYDPVP"
     gene            complement(769..1125)
                     /gene="rnpA"
                     /locus_tag="PKW60_12735"
     CDS             complement(769..1125)
                     /gene="rnpA"
                     /locus_tag="PKW60_12735"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPK00345.1"
                     /translation="MPGQNKFPRRERLTRQSEYLDVYRNGEKQVGRAFICYAVRRHGQ
                     GRKLGIAVSRKVGKAVVRNRLKRYIRETYRTHRAHLAEDAHIVFVARPAAAALDYAAC
                     EHAIRQLLRKGGLLGG"
     gene            complement(1149..1307)
                     /gene="rpmH"
                     /locus_tag="PKW60_12740"
     CDS             complement(1149..1307)
                     /gene="rpmH"
                     /locus_tag="PKW60_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011867659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPK00346.1"
                     /translation="MWLAEERVVKRTFQPSNIRRKRNHGFRARMATAGGRNVIRRRRQ
                     KGRKRLSA"
     gene            1759..2787
                     /locus_tag="PKW60_12745"
     CDS             1759..2787
                     /locus_tag="PKW60_12745"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00347.1"
                     /translation="MEKLGAAIIGSGWVAGEYIRAFTTNPSTELRAICSRTKEGAQAK
                     AQEFGVTCDVYTDYEKMAARDDIDIVVVASPPNLHTEHAVAAAQAGKHLILEKPMALN
                     IEDCRTIRDAVAKAGIKSVISFVLRWNPLYEIIKSLIADNAIGDVFLGEVDYFHGVGP
                     WYKLYSWNVKREVGGSSLLAAGCHALDGLRWFMGGEVAEVCQYSTFGKAFDFKEYEYD
                     PTTCTMLKFADGRIGKVTSCIECTQPYVFNINLVGTLGSIKNNQLYSKKKFPGQTGWA
                     TIPTVLPDSGDVTHHPFSLEVAHLLDCIAEDRESHCNAADAFISHEICLAADLSGLEG
                     KPITLPLK"
     gene            2789..>3150
                     /locus_tag="PKW60_12750"
     CDS             2789..>3150
                     /locus_tag="PKW60_12750"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HPK00348.1"
                     /translation="MKRFLAKHSPLVILAVLCLLIAALMPNFRKPGNLKKVAYRTPVF
                     GIMASGQTLVILTGGIDLSVGSVAALSGVVNAKTIKAMESRSLPASVSVGIAAGLAVA
                     TLCGVLCGFATAYGKIPA"
BASE COUNT          601 a          974 c          954 g          621 t
ORIGIN      
        1 aggcgatcgc caagctcctg ctgggcggcg aaccataagc caagcggata gatcgtgtcg
       61 gtgtcgggcc ggtcggaacg cgggacgagt tcaacgacgt cgttgccgtc tttgtcgagg
      121 ataacctcgg cgcgtttcag gcgtgcgccg atccgggtga agacgaggcg gagctggccg
      181 ttctcgatag tgacttcgtc ctcgggcagg acggactcgg gcacaggcgg cagctcgggc
      241 caggcggaag cggcgtccgt tccgtcagcc ggcgcagcgc cgtcctcgct gtgttcgggc
      301 agagacgctc ccggcgcggt ctggacttcg ggcgcggccg gctcggggcc gacggcaggg
      361 cgttgcatga aataattcaa ccagatgacg accaatacgg tcatcaggat gatgccgagc
      421 atctggtttt tcatcaaccc ctggtcttga ttgtcatcca ccctgtgttc tccctggcgg
      481 atcgctcctc gcgatacgcc gcaccctttc cggcacgagg gcccgggggc tcctgggttg
      541 gacgagcctg gcgtttacgg gacggggtcg tatccaccgc gactgcaggg atggcatcgc
      601 gcgattcgcc gtatggcgta gccgacgcct cgtatgacgc cgtatcgttc cagtgcctcg
      661 atggcgtatt gggaacaact gggcgtaaac cggcaactgg gtcccaggat ggctgacaga
      721 agaggtgaga tgccgtattg gtaaccgcgg ataagcacta ccaacacctt agccacccag
      781 cagccctccc tttcggagaa gctgtcgtat cgcgtgttca cacgccgcat agtcaagagc
      841 cgccgcggct ggccgtgcga cgaacacgat gtgcgcgtct tcggctaggt gtgcgcggtg
      901 ggttcggtaa gtctcgcgta tgtaacgctt gagacggttg cggaccacgg ctttgccaac
      961 ttttcgcgat acggcgatac ccagtttccg cccctgcccg tgccgccgga ccgcgtagca
     1021 gataaacgcg cggcccacct gtttttcacc attccggtat acatcgaggt attcggattg
     1081 tctggtgagc cgttcccgac gggggaactt gttctggccc ggcacaacag gctcatcact
     1141 cgtactcttt atgcgctgag ccgtttccgg cctttctgcc ggcgacggcg gattacattt
     1201 cgccctcctg ccgtggccat gcgcgcgcga aatccatgat tccgttttcg cctgatattc
     1261 gatggttgaa aagtacgttt cactactctc tcctcggcga gccacagggt tttgcatgtg
     1321 taactcatac cgccggaatg cgatatggcc accatcctcg ccggtgttca cgtgtaaaca
     1381 gtaggcaagt atagatcatg tatccggtct gagtcaaatg caatgcgatg cctcatccgc
     1441 ccgcagaatt cgcttcggcc ggagggcccg gtgcgtggaa aaaatgcgac tgccgcactc
     1501 ggcgggcgcc gttttttgcg cgaaccggtt cattgtgtct acaatggccg cttggggatg
     1561 ccgctgttgg aatgaacgtg aattgcgccg cgacggcgcc cggctggccc tcgtgccctg
     1621 gaagccggtt tcgccgcgta caacgcgtac acgcgacgga aaacgctggc gtccttcgag
     1681 atgcgggccg caggctgcgc tgcggacccg gagtgggaac agcatcaggc caacggcctc
     1741 acaagggagc gaaatacgat ggaaaaactt ggagcagcaa ttattggcag cggctgggtc
     1801 gccggagagt atattcgggc ctttacgacc aaccccagca ccgaattgcg cgccatttgc
     1861 tcgcgcacca aagagggcgc ccaggccaag gcgcaggaat tcggcgtgac ctgcgatgtc
     1921 tacaccgatt atgaaaagat ggccgcgcga gatgatatcg acatcgtggt cgtcgcatcg
     1981 cctcccaatc tccacacgga gcatgcggtc gcggcggcgc aggcgggcaa acacctcatc
     2041 ctcgaaaagc ccatggcgct caacatcgaa gattgccgca ccatccgcga cgccgtggca
     2101 aaggccggga tcaaatccgt catcagtttt gtgcttcgct ggaaccctct gtacgagatc
     2161 attaaatctc tcatcgccga caacgccatc ggcgacgtgt ttctgggcga ggtggactat
     2221 ttccacggcg tcggcccctg gtacaagctg tattcctgga acgtcaagcg cgaggtgggc
     2281 ggcagctcgc tgctcgccgc cggctgccac gcgctggacg gccttcgctg gttcatgggc
     2341 ggtgaggtgg ccgaagtctg ccagtattcg acgtttggca aagccttcga cttcaaggaa
     2401 tacgagtacg atcccaccac ctgcacgatg ctgaaattcg ccgacggccg catcggcaag
     2461 gtgacctcat gcatcgaatg cacccagccc tacgtgttca acatcaatct ggtcggcacg
     2521 ctcggcagca tcaagaacaa ccagctctac tccaagaaga aattccccgg gcagaccggc
     2581 tgggccacga tccccacggt gctgcccgac agcggtgacg tgacccacca cccgttctcc
     2641 cttgaagtgg cgcatctgct cgactgcatc gcggaggacc gggaatcgca ctgcaacgcc
     2701 gcggatgcct tcatcagcca tgaaatctgc ctcgcggcag acctctccgg cctggaaggc
     2761 aaacccatca ctctgccgct gaaataacat gaaacggttt ttggccaaac actcgccgct
     2821 ggtgatcctg gcggtgctgt gtcttctgat cgccgcactc atgccgaact tccgcaagcc
     2881 cggcaacctg aagaaggtgg cctaccgcac cccggtgttc ggcatcatgg cctcaggaca
     2941 gacgctggtg atcctcacgg gcggcattga tctgtcggtc ggcagcgtgg cggctctcag
     3001 cggcgttgtg aacgccaaaa ccataaaagc catggaatcg cggagccttc ccgcctccgt
     3061 cagcgtgggc atcgccgcgg ggctcgcagt ggccaccctg tgcggcgtcc tctgcggatt
     3121 cgcgacagcc tatggcaaaa tcccggcgtt
//
LOCUS       DAOPBQ010000455         3149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24516_length_3149_cov_3.411765, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000455 DAOPBQ010000000
VERSION     DAOPBQ010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24516_length_3149_cov_3.411765"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PKW60_12755"
     CDS             <1..643
                     /locus_tag="PKW60_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK00349.1"
                     /translation="EHLPDAPAYPCDVSSDSSIADFFGKVRHHTEQIDFVIHSVAYAD
                     REDLQRDFIGTPRENFAMAMDISAYSLVAVAREAAELMATGGSIVTMTYFGSEKVVPN
                     YNVMGVAKAALEASARYLANDLGPRGIRVNCISAGPLRTLSASAIAGARTIGRFVEKV
                     SPLRRNIEGSDVGHAAVYLVSDWSATVTGEVLHVDSGCNIMGMGLVENEPHGA"
     gene            687..1643
                     /locus_tag="PKW60_12760"
     CDS             687..1643
                     /locus_tag="PKW60_12760"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPK00350.1"
                     /translation="MAFTRPTNRQLVECVAAHLDVDVESISLAPIPSGKFNDSYYVLA
                     GGTEYVLRIAPSRNAVFCFYEREMMRQEPHIHQVLLERTTVPVARIYAFDDSHRIIPR
                     DFLIMERLPGLAMSELTHIDEPAILYQVGQALAQTHAQKGDRYGYVGAHHPMEPQHSW
                     ADAFHIMWRKLVDDVVSVGYYDDEERTLMLSTLDKYMRLFDRPVEAALLHMDVWAQNI
                     LVEGGNRLTGLLDWDRALWGDPEIEFAVLDYCGISKQSFWEGYGGSRDESGEARVRNF
                     FYLLYEMQKYIVIRHGRDHDANGAMAHKRQVLQIMRRIQETI"
     gene            2068..>3149
                     /locus_tag="PKW60_12765"
     CDS             2068..>3149
                     /locus_tag="PKW60_12765"
                     /inference="COORDINATES: protein motif:HMM:NF018471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HPK00351.1"
                     /translation="MTHDGSPAAFAARATLPSARSFLIARWLFLRCLAALYLVTFLSL
                     LWQIPGLLGENGISPVKENLRLAQFIPGVERYLVFPTLFWFDGSDAFLYLLCGAGVVS
                     SALVFLGVAPRLLVPLLWLIHLSFFTVGEDLMEFQWDALLLEIGFLALFLTPSGIYPG
                     LGRKEPPSALVLWLFRLMLFRLVFIAGAVRLHYVLLHGVTAFEVPFASQPVPTLLGWY
                     ANTTPVPFRIGLGAVVLASQLLVPFLVFMHRRVRVGAGGILVIQQLALMALGLHFMAG
                     LLTLAVCLTLMDDAAWQGLFPFARKWMRPDPASVKTRPAPLRSFATGVVSLPIITLSA
                     MQLLTPLVPERHWPVHARILAALTEP"
BASE COUNT          592 a          899 c          974 g          684 t
ORIGIN      
        1 cgaacacctg cccgacgcac cggcgtatcc gtgcgatgtg tccagcgaca gcagcattgc
       61 tgattttttc gggaaggtgc gccatcacac ggaacagatt gattttgtga ttcattcggt
      121 ggcctacgcg gaccgcgagg acctgcagcg cgacttcatt ggcacgcccc gcgagaattt
      181 cgcgatggca atggatatct cggcgtattc cctggtggca gtagcccggg aggccgccga
      241 actgatggcc acaggcggca gcatcgtgac catgacctat tttggttcgg agaaggtggt
      301 tcccaactac aacgtcatgg gcgtggcgaa agcggcgctc gaagcatcag cgcggtacct
      361 ggcaaacgac ctcggccccc ggggcatccg ggtgaactgc atcagcgcgg ggccgttgcg
      421 caccctgtcg gcgtcggcca ttgcgggcgc gcggaccatc ggccgcttcg tcgagaaggt
      481 ttcgcctctg cggcgcaaca ttgagggcag cgatgtgggc cacgcggccg tctaccttgt
      541 ttctgactgg tcggcgacgg ttaccggcga agtgctgcac gtggattcgg gatgcaacat
      601 catgggcatg gggcttgtgg aaaatgagcc tcatggggca tagtaaacag gcgccctcgt
      661 gaaaacgcta ctgtgggaga cgggatttgg cgtttacgcg gccgacgaac agacaactcg
      721 ttgagtgcgt ggccgcccat ctggatgtgg acgtagaatc catttccctg gcgcccattc
      781 cctcaggaaa attcaatgat tcgtactacg tgcttgcggg cggcacggag tatgtccttc
      841 ggattgcgcc cagccgcaac gcggtgttct gtttttacga acgggagatg atgcggcagg
      901 agccgcatat ccaccaggtg ctgctcgaac ggaccaccgt gcccgtggcc cgcatttatg
      961 cctttgacga cagccaccgc atcatacctc gggactttct catcatggag cgcctgcccg
     1021 gcctcgccat gagcgagctg acgcacatcg acgagccggc gatcctgtac caggtgggcc
     1081 aggccctggc gcagacccat gcccagaaag gcgaccgtta cggctacgtg ggcgcacacc
     1141 atccgatgga gccccagcat tcgtgggcag atgcgttcca tatcatgtgg cgcaagctcg
     1201 tggatgatgt ggtatcggtg ggatattacg acgacgaaga acggacgctg atgctctcga
     1261 ccctggacaa gtacatgcgg ctgtttgacc ggccggtgga ggcggcgctc ctccacatgg
     1321 acgtgtgggc tcaaaatatc cttgtggaag gcggcaaccg gctgactgga cttctcgact
     1381 gggaccgggc gctctggggc gatccggaaa tcgagtttgc agtgctggac tattgcggca
     1441 tctcaaagca gtctttttgg gagggctacg gcggcagccg cgatgagtca ggcgaagcgc
     1501 gggtccgaaa cttcttttat ctgctctacg aaatgcagaa gtatatcgtg atccgccatg
     1561 gccgcgacca cgacgccaac ggcgcgatgg cccacaagcg ccaggtactt cagatcatgc
     1621 gccgaataca ggagaccatc tgaacccggg gctgtacccc taaccctgag agcgggcaga
     1681 gatggaatgc atgctcccaa ggctgtgttc cgtaaaatga gtgcaggcgg gcacacaacg
     1741 gcgtgtcctt tggaacagca tgaaattgca gggacgttcc cgcagggcac gatgtaattg
     1801 aattcgtgac gagcgcgtgt ttttcatata cgattcaagc gcgattcaaa gcacaggatg
     1861 tagtaccacg caaagcagaa aatagtggcc gctgcgacag cggttttgag agatcaatat
     1921 cttcgcgcaa ttttccgcgc agccagcgcc tgccgcgcat ctgttcaccc gaaacaccgg
     1981 gtagaggcaa atcattgtga acaaaatgca cgacgccgcg tccattactg tatacctgaa
     2041 aatgcgacga ctactgtggt gacggatatg acacatgacg gcagcccggc ggcctttgcg
     2101 gcgcgggcaa cacttccttc agcccggagc tttcttattg cgcgctggct gtttctgcgc
     2161 tgcctggccg cgctgtatct ggtaacgttc ctgtcgcttc tatggcagat ccccggcctt
     2221 ctgggtgaaa acgggatctc gccggtcaag gagaatctgc gtctggcgca attcataccc
     2281 ggcgtggagc ggtatctggt cttccccacc ctgttttggt tcgacggaag cgatgccttc
     2341 ctgtatctgc tttgcggagc aggcgtcgtg agttccgcgc tggtatttct gggtgttgcg
     2401 ccgagactgc tggttccact cctatggcta atccatctgt cgtttttcac cgtgggcgaa
     2461 gatttgatgg aattccagtg ggatgccttg cttctggaaa tcggctttct cgcgctattc
     2521 ctcacgccgt cgggaatata cccggggctg gggcggaaag agccgccgtc ggcactggtt
     2581 ttgtggttgt tccggctgat gctgttccgg ctggttttta ttgcgggcgc ggtgcgtctg
     2641 cattacgtgc tgcttcatgg ggtcacagca tttgaagtgc cttttgcgag ccagccggta
     2701 ccgacgcttc tgggctggta cgcgaacact acgccggtgc cgttccggat tggcctgggc
     2761 gcggtagtgc ttgcaagcca gctcctggtc ccattcctgg tgttcatgca ccggcgcgtc
     2821 cgggtcggcg cgggtggcat cctggttata cagcaactgg cactgatggc gctggggctg
     2881 cacttcatgg ccgggctgct tacgctcgcg gtttgtctaa cgctgatgga tgacgccgcg
     2941 tggcaagggc tgtttccctt cgccagaaaa tggatgcgcc cggatcccgc atccgtaaaa
     3001 acacgcccgg cgccgctccg gagttttgcg acgggcgtag tgtcactgcc gatcattacc
     3061 ctctcggcaa tgcagctgct tactccgctc gtgcccgaaa gacactggcc ggtccacgcc
     3121 cggatcctcg cggctttaac ggaaccctt
//
LOCUS       DAOPBQ010000456         3128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24693_length_3128_cov_3.791084, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000456 DAOPBQ010000000
VERSION     DAOPBQ010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24693_length_3128_cov_3.791084"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PKW60_12770"
     CDS             complement(<1..888)
                     /locus_tag="PKW60_12770"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HPK00352.1"
                     /translation="MILTVTPNPCVDKTVFVDTLEVGTFMRSEKCTCIPGGKGTNVSR
                     AVKALGRPTRAMVVVGGHPGRHVVDMIEQQDGVDCLPAWVASQTRTITTVLETSVHRQ
                     TAFFEPGSRVTETEYEEIIARFRSAVNAVPVVTFNGSVCDKRIENLYRDLIGSAKEAG
                     ALTILDAHGPEFALGLESAPYMVKPNLAEAEEWAGHPLRTDDAKWEVIAQFHARGVEL
                     VALSLGERGAFVSRGGERLHVLPPAITEVNPVGSGDAFVAGFAIGLLEHMPLRDMAVL
                     ASAAGAANAMNWDIGHFRKD"
     gene            complement(896..1885)
                     /locus_tag="PKW60_12775"
     CDS             complement(896..1885)
                     /locus_tag="PKW60_12775"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HPK00353.1"
                     /translation="MTKAMIGIDLGGTFVKTAVVTREGEVLGKDSRPTDADQGLTAVL
                     DRMVEGVDAALQAANIQRPDVLAAGIGAPGPMNWQTGVVFAPPNLPGWKDVPLARLMS
                     ERLQLPVYVDNDANVACYGEFWLGAGRQVDSMVLLTLGTGVGGGIVLMGQLLRGPDGT
                     AGEIGHLCVKRDGRQCNCGARGCLEQYASVSGMLKTARKRIEQGGLNTLLLEMCGNEL
                     DNLTGKMISDAVKQGDTFARWVMEETGRWLGTGIGSIINLLNPEMVVLGGGMIGAGDV
                     LFNPVRETARAQSFEVPGKRAEIIPAGLGGDAGVIGAAGCALNRVEQDARAGK"
     gene            complement(1882..>3128)
                     /gene="gltX"
                     /locus_tag="PKW60_12780"
     CDS             complement(1882..>3128)
                     /gene="gltX"
                     /locus_tag="PKW60_12780"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPK00354.1"
                     /translation="EKGAYGPYRQSQRRELHRQEAFRLLEEGKAYKCFCTKEELEEMR
                     EQARQQKLPPRYNGKWRDASPDQIAAMGDAPYAVRFKVPEGETVIHDLVQGEVRTNNR
                     EYDDFVILKPNGDPIFHLAVVVDDGTMKISHVIRGDDHLTNAARHVMLFQALGYELPK
                     FAHLPMVLDETGKKFSKRLHGANVLDWRDDGYLPEALINYVVLLGWTPEEEGRELFTR
                     DELVQFFSVDRLGASPAKFDLKKLQWLNGQHIRRLSTDQLRDRVVPILQRAGFDTGAK
                     SEAWLTQMTGICQTKIPTLNDIVPYTDFFFVEPSEYEEKAVNKQWNKPGALDRMRTIR
                     DVLNGAASWDHDTLKAAYEKLSESTGEGLGQFVHPTRLACTGKSVGPGLFELLELLGK
                     DVSIERIDKAIRFIETMEEKTS"
BASE COUNT          512 a          968 c         1012 g          636 t
ORIGIN      
        1 gtcttttctg aaatggccga tatcccagtt catggcgttg gcggcgccgg cggcgctggc
       61 gaggacggcc atgtcgcgca ggggcatgtg ctcgaggagg ccaatagcga aaccggctac
      121 gaaggcatcg ccgctcccga cgggattgac ctcagtgatt gcgggcggca gcacgtggag
      181 ccgttcgccg cctcgcgaca cgaacgcgcc gcgttcgccc agcgaaagcg caacgagttc
      241 cacgccccgg gcatggaact gtgcgatcac ctcccatttg gcgtcgtcgg tgcggagcgg
      301 gtgtccggcc cattcctcgg cctccgccag attcggcttg accatatagg gggcggattc
      361 gagtcccagc gcgaattccg ggccgtgggc atccaatatg gtgagggcgc cggcttcttt
      421 cgcgctgccg atcaggtcgc ggtagaggtt ttcgatgcgc ttgtcgcaca cgctgccgtt
      481 gaaggtgacc accgggactg cattcacggc gctgcggaac cgggcgatga tctcttcgta
      541 ctcggtttcc gtgactcgcg aaccgggctc gaaaaacgcg gtctggcgat gcacggatgt
      601 ttccaggacc gtggtgatgg tgcgcgtctg cgacgccacc catgcgggaa gacagtccac
      661 cccgtcctgt tgctcgatca tgtcgacgac gtggcgcccg ggatgccctc cgacgacgac
      721 catcgctctg gtggggcgcc cgagggcttt aaccgcgcgg gagacgttcg tgcccttgcc
      781 gccggggatg caggtgcatt tttcggatcg catgaacgtg ccgacctcca acgtgtcgac
      841 aaagacggtc ttgtcgacgc aggggttggg ggtcacggtc aggatcatcg ttgcgtcact
      901 tcccggcgcg ggcgtcctgt tccacgcggt tcagggcgca gccggcagcg ccgataacgc
      961 cggcatcgcc gccaaggccc gcggggatga tctcagcgcg ttttccaggc acctcgaacg
     1021 actgggcccg cgcggtctcg cgcacgggat tgaacaggac atcaccagcg ccgatcatgc
     1081 cgccgccgag caccaccatc tccggattga gcagattgat gatgctgccg atgccggtgc
     1141 cgagccagcg cccggtctct tccatgaccc atcgcgcgaa ggtgtcgccc tgtttgacgg
     1201 catcggagat catttttccg gtgaggttat cgagttcgtt tccgcacatc tcgagcagga
     1261 gcgtattgag accgccctgt tcgatacgtt tccgggcggt cttgagcatg cccgagaccg
     1321 aggcatactg ctccaggcat ccgcgagcgc cgcagttgca ctggcgtccg tcgcgtttga
     1381 cgcagaggtg gccgatctcg cccgcggtgc cgtccgggcc gcgcaacaac tggcccatca
     1441 gcacaatacc gccgcctacc ccggtgccca gggtcaacag gaccatggaa tcgacctggc
     1501 ggccggcgcc cagccagaac tcgccgtagc aggcgacatt ggcgtcgttg tcgacgtaga
     1561 cggggagctg gagccgctcg ctcatgagcc tggccagagg aacatccttc cagccgggaa
     1621 ggtttggcgg ggcgaacacg acgccggtct gccagttcat cgggccgggg gcgccaatgc
     1681 cggccgcgag cacgtccggc cgctgaatgt tcgcggcctg cagggcggcg tcaactccct
     1741 cgaccatccg gtcgagcaca gccgtgagcc cctggtcggc gtccgtaggc cgcgagtctt
     1801 tgcccagcac ctcgccttcg cgggtcacaa cggcagtttt aacgaaggta ccgcccaggt
     1861 caatcccgat catggctttc gtcacgacgt cttttcctcc atggtctcga taaaacggat
     1921 ggctttgtca atgcgttcga tggacacgtc tttgcccagc agttcgagca gttcaaacag
     1981 gccggggccg acgctctttc ccgtgcaggc gaggcgtgtg ggatgcacga actggcccag
     2041 gccttcgccg gtgctttcgc tgagcttctc gtaagccgct ttgagcgtgt catggtccca
     2101 cgacgcggcg ccgttgagga catcgcgaat ggtgcgcatg cggtcgagcg cgcccggttt
     2161 attccattgt ttgttgacgg ccttttcttc gtattccgag ggttcgacga agaaaaagtc
     2221 ggtatacggc acgatgtcgt tgagcgtggg tattttggtc tggcagatcc cggtcatctg
     2281 ggtgagccag gcctccgatt tcgcgccggt gtcgaatccg gcccgctgga gaatgggcac
     2341 tacgcggtcg cgcaactggt ccgtgcttag ccggcggata tgctgcccgt tcagccactg
     2401 taattttttc aggtcgaatt tcgcgggcga ggccccgagc cggtccacgc tgaaaaattg
     2461 aaccagttcg tcccgggtga agagttcgcg gccctcttct tccggggtcc agccgaggag
     2521 gacgacgtag ttgatgagtg cctccggcag gtagccgtcg tcgcgccagt cgagcacgtt
     2581 cgcgccatgg aggcgtttgc tgaatttttt gccggtctca tcgagcacca tgggcaggtg
     2641 cgcgaatttc ggcagctcgt atccgagcgc ctgaaacagc atgacgtggc gcgcggcatt
     2701 cgtcaggtga tcgtcgccgc gaatcacatg actgatcttc atggtgccgt cgtcgacaac
     2761 gacagcgaga tgaaagatcg ggtcgccgtt gggtttcagg atgacgaaat cgtcgtattc
     2821 ccggttgttg gtgcgcacct cgccctgcac gaggtcgtgg atgaccgtct ccccttctgg
     2881 caccttgaaa cgaacggcgt agggcgcatc gcccatggcg gcaatctgat cggggctggc
     2941 gtcccgccat ttcccgttgt agcggggggg cagcttctgc tggcgggcct gttcgcgcat
     3001 ctcctcaagc tcttctttcg tgcagaagca tttgtaggcc ttgccttctt cgagcagacg
     3061 gaaggcctcc tgccggtgca actccctgcg ctgcgactgg cgatagggac cataggcgcc
     3121 tttttctg
//
LOCUS       DAOPBQ010000457         3119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24790_length_3119_cov_3.705940, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000457 DAOPBQ010000000
VERSION     DAOPBQ010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24790_length_3119_cov_3.705940"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            490..1569
                     /gene="recA"
                     /locus_tag="PKW60_12785"
     CDS             490..1569
                     /gene="recA"
                     /locus_tag="PKW60_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPK00355.1"
                     /translation="MAAKSDDSTARNKALDIAVAQLEKQFGRGTLLRLGDEHFVQKVG
                     AISTGSLSLDIATGVGGFPRGRVVEIFGPEASGKTTLALHAVANAQKQSGIACYIDAE
                     HALDPSFAAKIGVDVDNLLVSQPDTAEQALEICEALVRSNAVDIIVVDSVAAMVPKAE
                     VEGDMGDSHMGLQARLMSQALRKLTAAISRSKTLVIFINQIRMKIGVMFGSPETTTGG
                     QALKFYASMRIEIRRISSVKDKEENVGNRVRAKVVKNKLSPPFRSAEFEILFDEGISE
                     EGDVLDLAIADKLIQKSGSWFSLDGENLGQGRENTRIFLKEHPEIVERLKSDILKAKG
                     LTWLNSDEETSQDAAPQEEKADVSD"
     gene            1589..>3119
                     /locus_tag="PKW60_12790"
     CDS             1589..>3119
                     /locus_tag="PKW60_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00356.1"
                     /translation="MAPRVHSGRFTRIWLLALLVFSGLLAAVTLLIEYRLETIRQMAL
                     EAAEARTGIGLDADSVAVAGLRGIRIDGLRIDLAPEEGPVANIRIPSVYVHIDIVGLL
                     YGELNVERIELDHAVFTVRRPPGEAWYDQGRFPFHSSGAGPFTSLGAFRVLGRQCTLQ
                     VENVVRDTALAIHHLDFDVYRLPETEQVLANASGLLNGREESRLKANLTYVSPADFDL
                     KIESSQLTSADVNVFLPASKRFVETGLARPKARLVAYPDKTLMVSLETMFEDLTIRDH
                     PPFLPPATGNLDILASYNALSHELTLTSASAKSAQIGEGRLSGSVTLENEIPAFNLSL
                     ASSRLPLAEILDHAVMGRLQDYGKLDLQLAEPYEVVLSLDGNIEQFRIGAHANAAGGT
                     LEFEPSDPRLPTVHMTLDNLEGAWDSQERIARAALSIADGTHVCGQLHVAPTSPADSN
                     PGLCKGELYVDGLLVDSSDANGYKTSWLVGNQSEGAHTATYILYDRMLNEAPLSKTVY
                     VH"
BASE COUNT          656 a          969 c          881 g          613 t
ORIGIN      
        1 gcttgggaag ctggcgctct accaactgag ctaatcccgc tcgagtgact gtagtgtagc
       61 attgccacgg gcgggtgtca acgcaaatcc cggccggccg ccccccactc tcagcaagca
      121 ccgggaggag gcctggacca ggccgcagca tcatcccgat tgttgtgacg cgcccgggta
      181 tgcccggcct cgcgtgcgcc gccgctgctc tgcacgggtt taggctcaga tctgttttag
      241 cacctccgga agacccctgt tgcccttcgc agctctgtga cccgcggctt ttccacaccg
      301 tttcccgggg cacaaagatc aatcgtgcgg gatttttccc ccgggcgatt taaaaaagcg
      361 ttggacttct cctgaaaagc agctagaata gctggattcc aggtggccgc ttgacatgcg
      421 tcacagcttg tggcaccatg cgctttatag tgaaaatttt ttcagacaac atccaacgag
      481 gagcccgcca tggccgcaaa atcggatgac agtactgctc gaaacaaggc gctcgacatc
      541 gcggtcgcgc aactggaaaa acaattcgga cgcggaaccc tgctgcgcct gggtgatgag
      601 cattttgtcc agaaggtcgg ggccatttcg accgggagcc tctctctgga catcgctacg
      661 ggtgtgggtg gcttcccccg cggccgtgta gtggaaattt tcggccctga agcctctgga
      721 aagaccacac tggcactcca tgcggtggcc aatgcccaaa aacagagcgg catcgcctgc
      781 tacatcgacg cggaacatgc cctggatccc tcttttgccg ccaagatcgg ggtcgatgtg
      841 gataatctgc tggtatccca gccggatacc gccgagcagg cgctcgagat ctgcgaagcc
      901 ctggttcgca gcaatgccgt tgacatcatt gtcgtcgatt ctgtggcagc gatggtgccc
      961 aaagccgagg tggagggcga catgggagac tctcacatgg gcctccaggc acggctcatg
     1021 tctcaggccc ttcgcaaact taccgccgcc atcagccgtt caaagaccct cgtcatattt
     1081 atcaaccaga tccgtatgaa gatcggcgtg atgttcggca gtcctgaaac gaccacgggc
     1141 gggcaggccc tcaaattcta cgcttcgatg cgcatcgaaa tccggcggat ttcttccgtg
     1201 aaagacaaag aggaaaacgt gggcaaccga gtgcgcgcga aagtggtcaa gaacaaattg
     1261 agcccgccct tccgctcggc cgaattcgag attctgttcg atgaggggat ctcagaggag
     1321 ggcgatgtgc tcgatctggc tatcgccgac aagctgatcc agaagagcgg ctcgtggttc
     1381 tccctggacg gggagaatct ggggcagggg cgtgaaaata cccgcatctt cttgaaagaa
     1441 catcctgaga ttgttgagcg cctcaagagt gacatcctca aagccaaagg cctgacctgg
     1501 ctcaacagtg acgaagaaac cagccaggac gccgctcccc aagaagaaaa ggcagacgtc
     1561 tctgattaac gaagatcagc aggagcatat ggcacccagg gtgcattccg gccgtttcac
     1621 ccggatctgg ctgttggcgc tccttgtctt cagcggcctg ctggcggcgg tcacgctatt
     1681 gattgagtac cgcctggaaa ccattcgcca gatggccctc gaagcagccg aggcccgtac
     1741 gggtatcgga ctggacgcag actcggtagc agtcgcggga ctgcggggga ttcgcattga
     1801 cggtctgcgc atcgacctgg cgccggagga agggcccgtt gcaaacatcc gcataccatc
     1861 cgtatatgtc catatcgata tcgtcggcct tctctatggt gaattgaacg tcgagcggat
     1921 cgagctggat catgccgtgt tcaccgtccg caggccgccc ggagaagcct ggtatgatca
     1981 gggaagattc ccgttccact cgtccggcgc cggacctttc acttccctgg gagcatttcg
     2041 cgttctcggc cggcagtgca cccttcaagt ggaaaacgtt gtacgcgata ccgcactggc
     2101 catccaccac ctcgactttg acgtctacag gctgcccgag accgagcagg tgcttgcgaa
     2161 cgcatcaggc ctgctcaacg ggcgtgaaga aagccgcctg aaagccaatc tgacctacgt
     2221 ctcgcccgcg gacttcgatc tcaagatcga gtcctcgcaa ctcaccagcg ccgatgtcaa
     2281 cgtcttcctc cccgcgtcaa aacgtttcgt cgagaccggc ctcgcccggc ccaaggcgcg
     2341 cctggtggcc tatccggaca aaacgctcat ggtctcgctg gagacgatgt ttgaggatct
     2401 caccatccgg gaccatcccc ccttcctccc tcccgcgacg ggcaacctcg acattcttgc
     2461 ttcctataat gccctgtcgc atgaattgac cctgacctcc gcctccgcga aatccgcgca
     2521 aatcggtgaa ggcagacttt cgggaagcgt caccctggag aatgaaatcc cggctttcaa
     2581 cctcagcctc gccagttccc gcctcccact ggccgaaatt ctcgatcatg ccgtcatggg
     2641 gcggctgcaa gattacggta aactggacct gcaactggcc gaaccctacg aagtggtgct
     2701 cagcctggat gggaacatcg aacaattccg catcggtgcg catgccaacg cggccggcgg
     2761 gactctggaa ttcgaaccat cggatcctcg cctccccacc gtgcacatga cgctcgacaa
     2821 tctggagggc gcctgggact cccaggagcg catcgcacgg gccgccctca gcattgccga
     2881 cggcacgcac gtctgcgggc agctccacgt ggctccgacc agtccggcgg actccaaccc
     2941 gggtctgtgc aagggcgagc tttatgtcga cggtttgctg gtggactcca gcgatgccaa
     3001 cggctacaaa acctcttggc tcgtaggcaa ccaatccgag ggggctcaca ctgcgacgta
     3061 cattctctat gacaggatgc tgaacgaggc tccgctctcc aagacagtgt acgtgcaca
//
LOCUS       DAOPBQ010000458         3119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24792_length_3119_cov_3.100196, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000458 DAOPBQ010000000
VERSION     DAOPBQ010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24792_length_3119_cov_3.100196"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..757
                     /locus_tag="PKW60_12795"
     CDS             167..757
                     /locus_tag="PKW60_12795"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HPK00357.1"
                     /translation="MHSKVTTKQGDRGETTALNGGTYPKCHPIIHCAGALDEARAQTA
                     LLRQVLIAQNDTGYDGAVAFLHWVLHTFFVIGTECSDPERKHPEYRRGAIGEKELQRL
                     EAAQLRLEAQLALPRAFIVGASTIPAAHADVACTAVRRLERAFTALQQAVPGFDAGLL
                     PAFLNRLSDYLYILARWLERGVHLPVDYGLLGETEP"
     gene            complement(768..2390)
                     /locus_tag="PKW60_12800"
     CDS             complement(768..2390)
                     /locus_tag="PKW60_12800"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPK00358.1"
                     /translation="MPALSTAPVPPARPDSSTRRVAASLALMFCVLHAGATVMIYEFI
                     AVRILQRDFGSLLEIWAAEMTVVLAGLAIGYALGGYLADRFQAWWLLGLVLALGGVTA
                     LPMEPLAFRTADWLFSFENTEPAWWWPILAAGACTFVPILALGTVTPQAIRLQVRSFE
                     RLGSGAGWIASLSTVGSILGVLLITFAFLPNFGVRATLLWVSLALMALGLLVMAVSLL
                     TRRMGSAAALFLFTILGISGGADAQVLFDRYSAYHHILVVDDGDIRMLRFDDTRQSQM
                     SRRNPVEGGFEYTDFFHVPMIFNPAAEDILFVGLGGGTGPKAFLHYYPNTRLDVAEID
                     PLVVKVAREFFQVPEDPRLHISVADGRVFIRRTRKQYDAIMMDAYGTSPYGLSLPYHL
                     TTVEFFRIARERIKDGGCLLYNTVETDRRTQRDRVQDLLITMAQVFEYTYAFKARSSM
                     NTVLVGVKIPLNAEGVPEGKYAPWPHGPVAMHPLSAEQLAELTRRLADAGTLPLPRLQ
                     DRIRQISPVVNNPGRGMILTDDFAPTDLGAGG"
     gene            complement(2450..>3119)
                     /locus_tag="PKW60_12805"
     CDS             complement(2450..>3119)
                     /locus_tag="PKW60_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPK00359.1"
                     /translation="RRRHIPFNEKLDVGCMTTEFQLEAIENQIRDAESKGASIVLGGS
                     RNKESRVFPPTIVTELNENMSIARDESFGPVVTVNKFKTEQEAIELANDSPFGLSASV
                     WSADLERAERVARSLLTGNVSINNVLATQTNSGLPFGGVKDSGFGRYRGSFGLHAFSN
                     VKSLVIDKQSPKTELNWYPYSRKKLELFSELMAVAFSGKPFALLRTAVAGLRLERLAK
                     KNRL"
BASE COUNT          658 a          951 c          938 g          572 t
ORIGIN      
        1 tggcccccct ggcgcggcat gcgccagaaa aacatcggct ggcggctgga ttatcatgtg
       61 atcgacgaag ccctgctgcc caggctcaaa gaatcgcgga ttcatgccga agtgccgggc
      121 tcggaccatt gcccggtttc catcaccatc gattgagggc ggcgttatgc actccaaagt
      181 gacgacaaaa cagggcgacc gtggcgagac caccgcgctg aatggcggga cctatccgaa
      241 gtgccatccc atcattcact gtgctggcgc cctcgatgag gcccgtgcac aaacggcgct
      301 tctgcgccag gtgctgatag cgcagaacga cacaggctat gacggcgccg tcgcgttttt
      361 gcactgggtg ctgcacacct tctttgttat tggcacggag tgcagcgacc ccgaacgcaa
      421 gcaccccgaa taccggcgcg gcgcgatcgg cgaaaaggaa ctgcagaggc tggaagccgc
      481 gcaattgcgg cttgaagcgc aactggcttt gcccagggcg ttcatcgtgg gcgcatcaac
      541 gattcccgcc gctcatgcgg acgtcgcgtg caccgcggtt cgacggctgg aacgggcgtt
      601 tacagcgctc cagcaggccg tgccagggtt cgacgcgggg cttttgccgg catttttgaa
      661 tcgtctgagc gactacctgt acatcctggc acgctggctc gagcgtgggg tgcatctgcc
      721 ggttgattac gggctcctgg gcgaaacgga gccgtaacgc cgtccgctca tcccccggct
      781 ccgagatccg taggcgcaaa gtcgtccgtc agaatcatcc cccggcccgg attgttcacg
      841 acgggtgaga tttgccggat tcgatcctgc aaccgcggaa gcggaagcgt ccccgcatct
      901 gccaggcggc gggtgagttc cgcgagctgt tccgcgctca ggggatgcat ggccacgggg
      961 ccatggggcc acggagcata tttgccctct gggacgccct cggcattgag cggaattttg
     1021 acgcccacca ggacagtatt catgctgctc cgcgctttga aggcataggt gtactcgaaa
     1081 acctgggcca tggtgatgag taaatcctga acacgatccc gctgggtccg gcggtcggtt
     1141 tccacggtgt tgtaaagcag gcagccgccg tccttgatgc gctcccgcgc gatccggaaa
     1201 aactccactg tcgtgagatg atacgggagg ctcaacccat acgggctggt cccgtacgcg
     1261 tccatcatga tcgcatcgta ctgttttcgt gtccggcgga taaatacccg gccgtcggcc
     1321 acggagatgt gcagacgcgg gtcttcaggc acctgaaaga attcacgcgc aaccttgacc
     1381 accagcgggt cgatctccgc gacatcgagg cgggtattag gataatagtg caggaatgcc
     1441 ttgggtccgg tgccgccccc aagccccacg aaaaggatgt cttccgcggc cgggttgaaa
     1501 atcatcggga catggaagaa atccgtgtat tcgaagccac cctcgacagg attacgccgg
     1561 gacatctggc tctgccgggt atcgtcgaag cggagcatgc ggatgtcccc gtcgtcgacg
     1621 acaagaatgt gatggtaggc actgtagcga tcgaagagca cctgcgcgtc tgcaccgccg
     1681 ctgatgccca gtattgtaaa gaggaacagc gcggcggcgc tacccattct gcgggtcaga
     1741 agggacacgg ccatgaccag cagacccagc gccatgaggg ccagagatac ccacaggagg
     1801 gtcgcgcgca cgccgaaatt cggaagaaac gcaaacgtga tgagcaggac acccaaaatg
     1861 cttccgaccg tggataggct cgcgatccag ccggcgcccg agcccaggcg ctcgaagctg
     1921 cgcacctgca aacggatcgc ctgcggggtc accgtaccaa gggccaggat tggcacaaag
     1981 gtgcacgcgc ctgcggccag tatcggccac caccaggcgg gctcagtatt ctcgaaggaa
     2041 aacagccaat ccgcggtgcg aaatgccagc ggctccatgg gcagggcagt tacccctccc
     2101 agagccagca ccaagcccag cagccaccac gcctgaaagc ggtcggccaa ataccccccc
     2161 agcgcatacc cgatggccag tcccgcaagt accacggtca tttctgcggc ccagatctcg
     2221 agcaggctgc caaaatcccg ctgcagaatg cgcacagcga tgaactcata aatcatgacg
     2281 gtcgcgccgg cgtgcagcac gcaaaacatc agcgccagcg aagcggcaac acggcgtgtg
     2341 gaggaatcgg gtcgggccgg cggtaccggt gcagtggaca atgcaggcat cagagcctcc
     2401 tgaatcttgt acgttctcag aaaaacggcc cgcgggccgg gcaacacgat cacaggcgat
     2461 tctttttcgc taatcgttcg agccgcaaac ccgccactgc agtgcgcagc agtgcaaacg
     2521 gtttgccgct gaaagcgacg gccatcagct cgctgaaaag ctcgagtttc ttccgcgaat
     2581 aggggtacca gttcaactcc gttttggggc tttgcttgtc gataaccagc gactttacat
     2641 tcgagaaggc gtgcaggcca aacgaaccac ggtaacgccc aaaaccgctg tccttcacac
     2701 cgccgaaagg cagaccggag ttggtttgag tcgccaggac gttgttgatc gacacatttc
     2761 ccgtgagaag acttcgggcc acacgctcag cgcgttccag atcagccgac cacacgctgg
     2821 cactcagtcc gaacggagag tcgttggcga gttcaatggc ttcctgttcg gtcttgaact
     2881 tgttcacggt tacgacaggc ccaaagctct catcccgggc gatgctcatg ttttcgttca
     2941 actccgttac gatggtcggc ggaaacacac gggattcttt gttgcgtgac cctccgagaa
     3001 cgatgctcgc gccctttgac tccgcatccc tgatctggtt ttcgatggct tcgagctgaa
     3061 attccgtagt catgcagccg acgtcgagct tttcattgaa ggggatgtgg cgtcggcgc
//
LOCUS       DAOPBQ010000459         3116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24817_length_3116_cov_3.256779, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000459 DAOPBQ010000000
VERSION     DAOPBQ010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24817_length_3116_cov_3.256779"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKW60_12810"
     CDS             complement(<1..530)
                     /locus_tag="PKW60_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00360.1"
                     /translation="MKDASKHSVTRRDFAKVTAAGTFAVLGAKSASAAGRTLKVGLLG
                     CGGRGTQDAMNLLAGNEGVELVAMGDIFEDHLEDRRKQLVEHNDPNISSKVKVEDKNC
                     FVGWDAYQHILNTDIDIIMETSTPFIRPKHLEAAVEAGKHIFAEKPVATDTHGLQVFR
                     RAMAAHKAKGLSMVAGT"
     gene            866..2776
                     /locus_tag="PKW60_12815"
     CDS             866..2776
                     /locus_tag="PKW60_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00361.1"
                     /translation="MTHGTGSGIALLIAGVLVVTAGMAWAANEDLGNGFRHHGVATPV
                     STHRGTVATVDGNGNNVVLAWLFDHRGCYALLEIQAATGAAREIPVPFPPGGDCPFAS
                     ILSSKNRFYTHFNSYFTEYDAVKGEFTFFSPTAPQMAMSMTEDDSGVIWSATYPKSGV
                     VSFNPDTREFKDYGHVYAQNWAQYQRSVAADDNGWIYFGVGNTASQIIIFDPTTGTAT
                     PIVPEEQRVLGTGMVQRDVNGKVYGRSGSQWYELHQGQATEIEAPSITPKPIITSHQG
                     LFHNTFPDGKILQTCDLTERILEVKDPATGDVARFSFDYSSEGAHIMALAAASDGTIC
                     GGTAFPMRFFSYAPKTDEWVHRDAYGQWNAIATQGDRFFVGGYGGGFLLEWDPGAAWV
                     PTEKGNPECNPRYLTECTPVIHRPHDLLAHPDGKTLVLAGTPGYGYTGGGLLFWDRET
                     QNATVIEHTELIPQQSTTSLVALPDGKLLGGTTTMAGTGGEIKAAEAVLYILDTASRK
                     VDWQEAVLPGVQTYPDLFLAGSGLVYGVADGLRFFVFDPVARQLEHEEDLQNTLGATA
                     SQQQSRVFIAGPDQALYMLFVKGIVKVDYDTHALTLIGESPVPIGPGGTMLEGRIYFG
                     SGSHVYSYTLPE"
     gene            2926..>3116
                     /locus_tag="PKW60_12820"
     CDS             2926..>3116
                     /locus_tag="PKW60_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00362.1"
                     /translation="MQNILVAIWLLGAVDASSVQTLPGTAPLEATGDLAQAMVAGIDQ
                     WLDRALAASIDARKALWRR"
BASE COUNT          635 a          930 c          927 g          624 t
ORIGIN      
        1 gtgccggcga ccatcgagag gcctttcgcc ttgtgggcgg ccatggcccg gcggaaaacc
       61 tgcagcccgt gggtatcggt cgctacaggc ttctcggcga aaatgtgctt gcccgcttca
      121 acggccgctt cgagatgttt gggccggatg aagggggtgc tggtctccat gatgatgtcg
      181 atgtctgtgt tgaggatgtg ctggtaggca tcccaaccca cgaagcaatt tttgtcctcc
      241 accttgacct tgctgctgat gttggggtca ttgtgttcca ccaactgctt tctgcggtct
      301 tcgaggtgat cttcgaagat atcgcccatg gcgacaagct caacgccttc gttgcccgcc
      361 agcaggttca tggcgtcctg cgtaccgcgc ccgccgcagc ccaacaaccc cactttgagg
      421 gtccttccgg ccgccgacgc actcttcgct cccagaacgg caaatgtgcc cgctgccgta
      481 actttggcga aatcgcgccg ggtaacactg tgtttcgatg cgtctttcat tgatactctc
      541 ctaatcggtc ctgatgcgta tccccttcag gaggaccgga tcccaccacg atccgaagcc
      601 cgccttccat ggtttcaaca atcgaattca caccatatca caccaccccg gtaacgcgcg
      661 aatccacgca caagcgcaac aggaaccctc gaaggttgcc cggatcctcg agtctaagct
      721 gtggggaatt cagacttggg gagcgcagat cgccactcgc ggtgagatta cgtatgcctt
      781 gcgccgtcgc ttctatcgtg cgaagattgc aaagagaaca caaaaagaag ctggcacggc
      841 gtcagaccaa atggaggaat ctcgcatgac gcatggaaca ggatcgggta tcgcattgct
      901 gattgcgggt gtgcttgtgg tgacggcggg gatggcctgg gcggcgaatg aagacctggg
      961 caacggattc cgccaccacg gcgttgctac cccggtgagc actcatcggg ggacggttgc
     1021 aacggtagac ggcaacggaa acaacgtcgt cctggcatgg ttgttcgacc accgcgggtg
     1081 ctatgcgcta ctggagatcc aggcggcaac gggcgcggcc cgggagatcc ccgtgccgtt
     1141 cccgccgggc ggagattgcc cgttcgcatc aatcctgtcc tcaaagaacc ggttctacac
     1201 gcatttcaac agttatttca ccgaatatga tgcggtcaag ggcgaattca cctttttcag
     1261 ccccactgcg ccgcagatgg ccatgagcat gaccgaagac gattccggcg tgatctggtc
     1321 ggcgacgtat cccaagagtg gcgtcgtttc gtttaatccc gacacgcgcg agttcaaaga
     1381 ctacggtcac gtctatgctc aaaactgggc ccagtaccag cgatccgtcg cagcggacga
     1441 taacggctgg atctattttg gcgtggggaa taccgcgagc cagatcatca tttttgatcc
     1501 cacaacggga acggctacgc ccattgtgcc ggaagagcag cgtgttcttg gaaccggcat
     1561 ggtacagcgc gatgtgaacg gcaaagtcta tggccggtcg ggcagccagt ggtatgagct
     1621 gcaccagggt caagccactg agatcgaagc accctccatt acgcccaaac cgatcattac
     1681 gagccaccag ggcctgttcc acaatacttt tcccgacggc aaaatccttc agacctgcga
     1741 tctgaccgag cgaattctcg aggtcaagga tcccgcgacc ggcgacgtgg cgcggttttc
     1801 gttcgattac agcagcgagg gtgcgcacat catggccctg gcagccgctt cggatggcac
     1861 catttgcggc ggcaccgcgt ttccaatgcg cttcttcagt tacgctccca agaccgacga
     1921 atgggttcac cgcgacgcct atggccaatg gaatgccatc gcaacacaag gcgatcgctt
     1981 ttttgtgggc ggctatggcg gcggattcct gctcgaatgg gatcccggcg cagcgtgggt
     2041 gcctacggag aaaggcaatc cggaatgcaa tccgcgatac ctcacagaat gcacgccggt
     2101 catccatcgg ccgcatgatc tcctcgcaca cccggatgga aagacgctcg tgctcgcagg
     2161 cacgcccggt tatggatata cgggcggcgg actgcttttc tgggatcgag aaacgcagaa
     2221 tgcgacggtc atagaacata ccgagctgat tccccagcaa tcaacgacca gcctcgtggc
     2281 cctgcccgac ggcaaacttc tgggcggcac gacaaccatg gcggggacgg gcggcgaaat
     2341 taaggcggcc gaagccgtgc tgtacatttt ggacacagcg agccgcaagg tcgactggca
     2401 ggaggccgtg ctgccgggag tacagaccta tccggaccta ttcctggccg gcagcggact
     2461 ggtatacggt gtggcggacg gcctccgttt tttcgtgttt gatcccgtgg cacgccagct
     2521 tgagcatgaa gaggatcttc aaaatactct cggcgctacc gcaagccagc agcagtcccg
     2581 cgtatttatc gcgggcccgg atcaggcgct ctacatgctg tttgtcaaag gcattgtgaa
     2641 ggtcgactac gacacgcatg cgctcacttt gatcggggaa tcgcccgtgc ccatcggccc
     2701 cggcggaacc atgctcgagg gccggattta tttcggcagc gggtcgcacg tgtacagcta
     2761 cacccttccc gaataagaca aaacctgccg cgactgcatg aatgcgtcag gcaacggcgc
     2821 gtggccagag cttcctcaag gctgcttcgc cgcgcgccgc attgactcca tctccggtgc
     2881 gtcgtactgt gtaggtaatc cggcgggaac ccagaggagc atgccatgca aaatatcctg
     2941 gtcgcgatct ggttgctggg ggcggtagat gccagttcgg ttcagactct acccggtacc
     3001 gccccgctcg aggcaaccgg cgacctggcg caggcgatgg tcgcggggat cgaccagtgg
     3061 ctggatcgcg cgctcgcggc ctcgattgat gcgcgaaagg ccctgtggcg gcgcga
//
LOCUS       DAOPBQ010000460         3112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24856_length_3112_cov_4.482826, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000460 DAOPBQ010000000
VERSION     DAOPBQ010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24856_length_3112_cov_4.482826"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2487)
                     /locus_tag="PKW60_12825"
     CDS             complement(<1..2487)
                     /locus_tag="PKW60_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00363.1"
                     /translation="MPAVTRSLVYLAAALSFLCLSPRVLAQDPAIATFDLGTAQLSID
                     ALGYPTLELADTDAPWPAAAQPLVCLDTEGGLLRPERVTAENGRLTAQFPGGALCEWI
                     VTPGAGFLIFEVTRLDVPEAARLKLCSLRAPENAEIMSTLNAASTPTHIVALSAAEVN
                     VLAHIRAIGRRDGDKAGCSHEFQPTDDARVGATAARFSATSGGEAGGWSVVGHDFLRP
                     LDLTGCTAIRAWVHGDGKGQQLKIQLNDGRGGYRDTYIPITFQGWQHITIDTPALDTL
                     RYDNAVTLSVYYNSLPAGESVSCLVDHIEALVERDGKQEAILLEDFERPDSLFWQAKA
                     YTLNLETLTSHGLLPARFGLLASPQKTFLDTMERFEIAAGLPSPHPDGVWNKRSPWIK
                     RSYLFITRFDASQTDEVIALARRGGFDMILILQSSWTRATGHFEINTDNFPGGLETLA
                     GTVQRLKDAGFHVGFHMLGASIYPPDRYLTPVPDPRLVKGASCELAADLDEKADFFPT
                     IAVPEGFPAEDGGYEGDGTVIQVGNELIQYQSLNLEPPTGFAGCRRGHLGTTAAAHAQ
                     GTPITHIRRAYGYHMYDMDTTLIDEISTHFAHVANACDIDMIYFDGSERLQGDHWYYN
                     AKLHKTFFDKLARKDILIQASSFSHYSWHILARSASADGHGDLKGYLDERSGWLDAFK
                     RNAMPLDIGWYYGYDPNCTLDMYEYVLGATIGYDSSMSFQVSLDAARKHPFTGDILDC
                     IARYERLRLSGRVPAEMRERLRIEPTLAGKKSPEERAALLDLRREYRLLGPEGEEVFQ
                     RVMYSPWQEVNAAAPESVQWRVHVPAGPARV"
     gene            complement(2580..>3112)
                     /locus_tag="PKW60_12830"
     CDS             complement(2580..>3112)
                     /locus_tag="PKW60_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00364.1"
                     /translation="GPANPLFSASTGYSATMWGFPYDDLKGWRGPYPPDIFASQFEKV
                     AQGWQPGLTELETALQKTPEHLRTEAAGDLRLARAAAINFQSVANQARFVMARDALAE
                     AGDALEPEQREQHLAELRKVLESEIDLARQLFTLTREDSRIGFEPSCQYFYLPLDLVE
                     KVLNCRWLLEFWGLNS"
BASE COUNT          587 a          897 c         1038 g          590 t
ORIGIN      
        1 gacgcgcgca ggacccgccg gtacgtgtac tcgccattgc acgctctccg gggcggcggc
       61 gttcacctcc tgccagggac tgtacatgac gcgctgaaag acctcctcgc cttcggggcc
      121 caggaggcgg tattcacggc ggagatccag gagcgcggcg cgctcttccg gtgatttctt
      181 tcccgcgagc gtgggttcga tgcgcagccg ttcgcgcatc tccgcgggta cccggcccga
      241 cagacggaga cgttcatagc gcgcgatgca gtccagaatg tcgcccgtga acggatgttt
      301 acgcgcggca tcgagcgaca cctggaacga catcgacgaa tcgtaaccga tcgtggcgcc
      361 gaggacgtat tcgtacatgt cgagagtgca gttggggtcg tagccgtaat accagccgat
      421 gtccaggggc atggcattgc gtttgaacgc atcaagccac cccgaccgct catcgaggta
      481 ccccttgaga tcgccgtggc cgtcggcgga cgcgctgcgg gcgagaatgt gccacgagta
      541 atgactgaag ctgctggcct ggatcaggat atctttgcgg gcgagcttgt cgaagaaggt
      601 tttgtggagt ttggcattgt agtaccagtg atcgccctga agacgctctg aaccgtcgaa
      661 gtagatcata tcgatatcac aggcattggc aacgtgggcg aagtgcgtgg atatctcgtc
      721 gatcagagtc gtgtccatgt cgtacatgtg gtagccgtaa gcgcggcgga tgtgcgtgat
      781 tggcgtgccc tgcgcgtgag ccgcggctgt ggtgcccaga tggccgcgcc ggcagcccgc
      841 aaacccggtc ggaggctcca ggttgagcga ctggtactgg atcagctcgt tgccgacctg
      901 aatcaccgtt ccgtcgcctt catatcctcc atcttccgcg ggaaaacctt cgggaacagc
      961 gatcgtaggg aagaagtcgg ctttttcgtc cagatcagcg gcaagttcgc aggaagcgcc
     1021 tttcaccagg cgcggatccg gcacgggggt caggtatcga tcgggcgggt agatggaagc
     1081 gcccagcatg tggaatccga cgtggaatcc cgcatctttg agtcgctgca cggtgccggc
     1141 cagggtctcg aggccgccgg gaaaattgtc cgtgttgatt tcaaaatggc cggttgcccg
     1201 cgtccaggag ctctggagga tgaggatcat gtcgaatccg ccgcgccggg ccaatgcgat
     1261 gacctcatcc gtctgcgagg cgtcgaagcg ggtgatgaag aggtaggagc gcttgatcca
     1321 gggcgaacgc ttgttccaga cgccatcggg atgggggctg gggaggccgg cggcgatctc
     1381 gaagcgctcc atggtgtcga gaaacgtctt ctgcgggctg gccaggagcc caaagcgcgc
     1441 gggcaggagt ccatggctgg tcaaggtctc gagattcagg gtataggcct tcgcctgcca
     1501 gaacagagag tccgggcgct caaaatcttc gagcagaatg gcctcctgtt ttccatcgcg
     1561 ctcgaccagg gcttcgatgt gatcgacaag gcaggacacc gattcgcccg cgggcaggct
     1621 gttgtaatag acgctgagcg tgacggcgtt atcgtagcgc agggtgtcaa gcgcgggcgt
     1681 atcgatcgtg atgtgctgcc agccctggaa agtgatgggg atgtaggtgt cccggtagcc
     1741 gccgcgcccg tcgttgagct ggattttgag ctgctggcct ttaccgtccc cgtgaaccca
     1801 ggcgcggatg gcggtgcagc ctgtgagatc cagaggacgg aggaagtcat ggcccacgac
     1861 gctccagccg cccgcttcgc cgccggatgt ggcgctgaac cgcgccgcgg tggcgcccac
     1921 ccgggcatcg tccgtgggct gaaattcatg ggaacacccg gctttgtcgc cgtctctgcg
     1981 gccgatggcg cgtatatggg cgagcacgtt cacttcggct gcggataagg caacgatgtg
     2041 cgtgggcgtg cttgcggcgt tcaatgtgga catgatttca gcgttctcgg gcgcgcgaag
     2101 cgagcaaagc tttaaacgcg ccgcctcggg cacgtccagg cgggtgactt caaagatgag
     2161 aaagcccgcg cccggggtga cgatccattc gcacaaagcg ccgccgggga actgcgccgt
     2221 cagccgccca ttctccgccg tcacgcgttc cggacgaagc aggccgcctt ccgtgtccag
     2281 acagacgagc ggctgggctg cggccggcca gggcgcatcg gtgtccgcca gttcgagtgt
     2341 tgggtaaccc agggcgtcga tcgagagctg tgcggtgccc aggtcgaacg tagcgatggc
     2401 cggatcctgt gccaaaacac gcgggctgag acacagaaaa ctcagcgcgg ccgccagata
     2461 aacaagggat cgcgttaccg caggcatgtc agtcctcccg caggtcttgt ctgcacgaaa
     2521 cagagttgtc cctgcaagtc gccgcatcaa aagccgatgt gcttacccta gacgttttct
     2581 taggaattca gtccccagaa ttccaggagc cagcggcagt tcagcacttt ttccacaaga
     2641 tcgagcggca gataaaaata ctggcacgac ggctcaaacc cgatgcgtga gtcttcacgg
     2701 gtaagggtga agagctgccg ggcgagatca atttcggatt ccaggacctt ccgaagctcg
     2761 gccaggtgct gttcccgctg ttcgggttca agcgcatccc cggcttcggc gagggcgtcg
     2821 cgtgccatca cgaaccgggc ctgattggcc accgactgga aattgatggc ggcggcacgg
     2881 gccagacgaa ggtcgccggc ggcttcggtg cggagatgct caggtgtttt ctgcaacgcg
     2941 gtttccagct cagtcagacc cggctgccag ccctgcgcca ccttttcaaa ttgcgacgcg
     3001 aaaatgtccg gcgggtaggg tccgcgccag cctttcagat cgtcgtaggg aaatccccac
     3061 atggtcgcac tgtaaccggt ggaggctgaa aagagagggt tggcggggcc gc
//
LOCUS       DAOPBQ010000461         3110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_24877_length_3110_cov_4.115548, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000461 DAOPBQ010000000
VERSION     DAOPBQ010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24877_length_3110_cov_4.115548"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(367..1374)
                     /locus_tag="PKW60_12835"
     CDS             complement(367..1374)
                     /locus_tag="PKW60_12835"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HPK00365.1"
                     /translation="MRVGGMIAMGLWVAALASCQTGPAESGTAARQAAKDSAGGFRFE
                     DNGQSLTLLEDGKPVLTYRYTFVEPPAGVDGIYRRMSYIHPLYGLDGDVLTQDFPDDH
                     YHHRGIFWAWPRCAVGERPMDVWLGTEAKQIFDRWVARKAEADTATFAVENLWVFDDR
                     APKVRERAGFIVHRANDLGRAIDVCLKFTNVCDEVVTIRGATEQNKGYGGLCYRPDAA
                     RKPMMFTTAQGGIDADQTEYDTPWADCTSAAPNGPESGLAIFQHPGNPGYPHSGWLFR
                     HYGFLGASYPHNETLTLNPGDSFEVRYRLYVHRGNAASGQVAEQFAQYTEAMKQGGAE
                     P"
     gene            1862..>3110
                     /locus_tag="PKW60_12840"
     CDS             1862..>3110
                     /locus_tag="PKW60_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPK00366.1"
                     /translation="MKRCLPVLSIAVTLSWLAIPALAAEAGAAMPPIAERVKLPAWPE
                     PAVVLHVAPGGSDEASGTAEAPFATLERARDMLRALKKEGRLAHGGAVVVIHGGDYQV
                     SQTFVLSAEDSGEASAPIRYEAVGGETPVFRGGIRIDQFEKVTDEAILSQWPEEVRGK
                     VVQTRLAQYGIGDLKPLRLGGFNSGLGFETHPTVELFFNGEPMTLARWPNEGFVQVAG
                     VAVEDGHSIHGLKGSKIGRLFYEGERPARWKDEPEVTLYGYWFFGWADSYERVASIDT
                     ERHEFVLEEPYCGYGYRAGAPYYALNLLSEIDKPGEWYLDRAAGILYFYPPANPSEAI
                     VELSLIDFPFVQANNVSHTSFRGLVWELGGANGIEIRGGSHGLIAGCTVRRCAGDGIV
                     VAGGNSHTLLGCNVYSMGRGGMMV"
BASE COUNT          577 a          930 c          955 g          648 t
ORIGIN      
        1 cacgccgggc gatatctgca acgattccat ccaaaccacg aggcttcgcc gcctgcagcg
       61 attggcccca cccgccgcgc gccagaaaca acaacgcctc accccggttc aattcccgtg
      121 cttcaggcag gagccgacta aactgtcaat agattgaacg ctttagatag aagaaaccat
      181 tcccagcacc gtcaaccaat gacctccagc gcggttcacg gaggctggac acttgtcaca
      241 gaaatcgtag gccaagcccg gtcgctttgt caatcggcta gagctttctt aatccatgtt
      301 ttatggattt aaaggtgctg cccgtggccg ttcccgtgcg ctgcttctcc aggtctgacg
      361 cggccgtcac ggctcggcgc cgccctgctt catcgcctcc gtatactggg caaactgctc
      421 tgcaacctgg ccagaggcgg cgtttccgcg gtgtacgtag aggcggtacc ggacttcaaa
      481 gctgtcgcct ggattcagtg tcagggtttc attgtgcggg taggaggcgc ccaggaatcc
      541 gtaatgcctg aaaagccatc cggaatgagg atagcctgga tttcccggat gctggaagat
      601 ggccaggccg gattccgggc cgttgggtgc ggcggaagtg cagtcggccc agggggtatc
      661 gtattcggtc tgatctgcat cgattccgcc ctgcgccgtg gtaaacatca tgggtttacg
      721 cgctgcatcc ggacggtaac agagcccgcc gtagcctttg ttttgctcgg tggcgccgcg
      781 aatggtcacg acttcgtcgc atacattcgt gaatttcagg cacacatcga tggcgcggcc
      841 caggtcattc gcgcgatgca caatgaatcc cgcgcgctcg cgcactttgg gcgcacggtc
      901 gtcgaacacc cagagatttt ccacggcaaa ggtggcggtg tctgcctccg cttttcgtgc
      961 cacccaccgg tcaaaaatct gcttggcctc cgtacccagc cagacgtcca tgggccgttc
     1021 gccaaccgcg cagcgaggcc aggcccagaa aattccgcga tggtgatagt ggtcgtctgg
     1081 aaaatcttgt gtcagcacgt cgccgtccag gccatacagc gggtggatgt agctcatgcg
     1141 ccggtatatc ccgtctactc cagcaggagg ctccacgaac gtgtaccggt aggttaatac
     1201 cggctttcca tcctccagca gggtcaggga ttggccgttg tcttcgaatc tgaatccccc
     1261 tgccgagtcc ttcgcggcct ggcgcgctgc agtccctgat tcagccggcc cggtctggca
     1321 cgacgccagc gctgccaccc ataatcccat cgcaatcatg ccgcccactc tcattcttct
     1381 ctccttccta aaagcccatc gcgcgccagt ctaccacagt cagaccgtga aaaacggggc
     1441 cagtgcagag cgcgccagct acgattaccg accttggcaa gagtccaccc tcggccacgc
     1501 gtgccgcgcg gccaccccgt ccaacacgaa gcgcgtgtgt aagggcaggc tcctgtgcct
     1561 gccctgtgtc aaggtcaacg cgtgccgatg agatgggcgg ccacaggggg ccgcccctgc
     1621 aatccggatg cggcgcactt gtccctggcg ggggtgaatc ggcgtcagcc gatttggggg
     1681 tggagttttt gaagtggatt gcgatctccg gaattgcagt tcgcgaggtc caattgcaac
     1741 aatgttgcgc ggactcaatt caatagggcg aaatctctgt gcagtctttc actgactcat
     1801 gagcctcgtc ggacctgtag gtcctatccg gttttcaacg cgcaagagga gggttgatca
     1861 catgaaacgg tgtctcccgg tgctttctat tgccgtcact ttgtcctggc tggcgattcc
     1921 ggccctggcc gccgaggccg gcgccgccat gccgcccata gccgagcgcg tgaaactccc
     1981 tgcctggcct gagccggccg tggtgctgca tgttgctcct ggcggctccg acgaggcctc
     2041 cgggaccgcc gaggcgccgt ttgcaacgct cgagcgggcg cgggacatgc tgcgtgccct
     2101 gaaaaaagag ggccgattag cccatggcgg cgcggttgtg gtgatacatg gaggagacta
     2161 tcaggtctcc cagacgttcg tgttgagtgc cgaagattcc ggggaagctt cggctccgat
     2221 tcgctacgaa gctgtcggcg gcgagacacc tgtgtttcgc ggcgggatcc gcattgacca
     2281 gtttgagaaa gtgaccgatg aggcaatttt gtcacaatgg cccgaggaag tccgtggcaa
     2341 agtggtgcag acccgcctcg cgcaatacgg catcggcgac ctgaaacctc tgcgcctggg
     2401 cggtttcaac agtggactcg gttttgagac gcatcccacc gtggagcttt tcttcaacgg
     2461 cgaaccgatg acgctggccc gctggcccaa cgaaggattc gtgcaggtgg ctggcgtggc
     2521 ggtcgaagat ggtcactcga ttcacggtct gaaaggcagc aagatcggcc ggctgttcta
     2581 tgaaggcgag cgccccgcgc gatggaaaga tgagccggag gtcacgcttt acggctactg
     2641 gtttttcggc tgggcggatt catacgagcg ggtggcttca atcgacaccg agcggcatga
     2701 atttgtgctg gaagaacctt attgcggcta cgggtaccgg gccggtgcgc cgtattatgc
     2761 cctcaatctg ttgagcgaaa tcgataagcc tggtgaatgg tacctggatc gcgccgcggg
     2821 cattctctat ttctatccgc cagccaaccc gtcggaagcg atcgtcgagc tgtcgctgat
     2881 cgacttcccg tttgtgcagg ccaacaacgt gtcgcacacg tcgttccggg ggctggtatg
     2941 ggagttgggc ggcgcgaacg gcatcgagat tcgcggtggc agccacggcc tcatcgccgg
     3001 ctgcaccgtg cgccgctgcg ccggtgatgg aattgtcgtg gcaggtggaa attcacacac
     3061 gctgctgggg tgcaacgtgt attccatggg ccggggcggg atgatggtgt
//
LOCUS       DAOPBQ010000462         3097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_25024_length_3097_cov_3.428994, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000462 DAOPBQ010000000
VERSION     DAOPBQ010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25024_length_3097_cov_3.428994"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKW60_12845"
     CDS             <1..252
                     /locus_tag="PKW60_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK00367.1"
                     /translation="HYPDEMSAGERQRCALARTLLKQPSVILADEPTGNLDPASADTV
                     LEHLDRARKRGATVLLVSHQPMDSIQPDVTFELREGRLV"
     gene            897..1322
                     /locus_tag="PKW60_12850"
     CDS             897..1322
                     /locus_tag="PKW60_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPK00368.1"
                     /translation="MTNSSNVTYSVNIDSPIAVYVQIENQIQFAIASGRIKPGFTLPS
                     VREMSEVLEVNPNTVTKAYRDLELLELVHTRRGVGVTIAEKAPKICRERVRPMVKSHL
                     VDAVAECLACGLTESDVRSIVESTISAGTCPYEAPKKKK"
     gene            complement(1345..2136)
                     /locus_tag="PKW60_12855"
     CDS             complement(1345..2136)
                     /locus_tag="PKW60_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK00369.1"
                     /translation="MSIVHVDGIGFDRKGVAILRGVSWRIGHGSHWALLGANGAGKTT
                     LLKIITGYEWPSRGSVHVLGQRFGECNIPELRKMVGWVSSAIESRLPEDDTALDVVSS
                     GLDASLGVYRAFSDLERARAHGALERVNGSILAGQTYATLSQGECQRVLIARALIANP
                     RLLVLDEPCVGLDPAARWRLLEDLAALAGSPDAPTVIYVTHHIEEIGDWIDNVLVLKD
                     GAVLAQGSREEILCASVLSQAFGLPCRVEKTREAYTLHIVPASRG"
     gene            complement(2182..2838)
                     /locus_tag="PKW60_12860"
     CDS             complement(2182..2838)
                     /locus_tag="PKW60_12860"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPK00370.1"
                     /translation="MLPSDAYAEERDAMVRHHIAGRGVTDPRVLAAMRTVPRHEFVPV
                     GKASCAYEDRPLEIGSGQTISQPYIVAKMTELLELTPHSRVLEIGTGSGYQAAVLAEL
                     AAEVISVERFRHLAEDARKALESLEYSNVSVVIGDGTLGWPERAPYDGIIVTAAAPAV
                     PPSLQSQLAPGGRLVCPVGSRELQQLKVLVRGPEDRFSEFESIRCMFVPLVGREGWSV
                     "
     gene            complement(2851..>3097)
                     /locus_tag="PKW60_12865"
     CDS             complement(2851..>3097)
                     /locus_tag="PKW60_12865"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HPK00371.1"
                     /translation="TLYMMMILLRWFGPWLEVDTDAGRLAWIGRATEPLIQRVRRILP
                     PMGPVDFGPLAALFMVWLARSIAMLLFAMERGATRTL"
BASE COUNT          674 a          977 c          905 g          541 t
ORIGIN      
        1 cactaccccg acgagatgtc ggcgggcgag cgccagcgct gtgccctggc ccggactctg
       61 ctcaaacagc cctcagtcat cctggcggac gagcctaccg gcaacctgga tccggcaagc
      121 gcggacaccg tgctggaaca tttggatcgc gcacgaaagc gcggggccac ggtattgctc
      181 gtgagccatc aacccatgga cagcatccag ccggacgtca ccttcgagct gcgcgagggc
      241 cgcctcgttt agcatggcag gttgcctcca gcgtgctggc tcagggtggc gccaggctgt
      301 cacagagccg cagcgaaagc cgaactcagc tccatgcagt cgggcgaata agctctccat
      361 gcgggagcac gcggcccaag agcctcgcga aactgctcga cgggctcacg aattcgggat
      421 tttccactct ttatctcctg ttttacccgt tatttgcgtc ttaatgagac ctgtaaagcg
      481 agaaaggccc ggataccaaa accggctaca agattaacac ttgacttcct acaattctat
      541 acgataccct aagtcttata acgggtgcgg aacaacctgc atatccgggt agataaacgc
      601 ccgtaaaaca ggttcatgta tctgccctgt atcggcacag ccgctgggaa gccgtcgcgg
      661 agacggagtg gggggcggct gaagagactg aaacctttta gtctctaaag ggatagtact
      721 tgagggcttg aatgcatgaa gagtatccgc acggtgcagt agtgaggata acctggctgc
      781 aagactttct gtgagaagtg gtggcaacag gcggtacaag aaaccgcggg cgaaaggttc
      841 acacggcgct cgtcccgcac tgtatgaacc cgaggcctta aacgcagagg tggaaaatga
      901 ctaacagtag caatgtgacc tactccgtca acatagacag tccgattgcc gtctatgtcc
      961 agatcgaaaa ccagattcaa tttgcgatcg catccggccg catcaagcca ggattcaccc
     1021 tgccttccgt ccgagagatg tcggaggtgc tcgaagtcaa tccgaacacg gtcacgaaag
     1081 cttaccgcga cctggaactg ctcgagctgg ttcacacgcg ccgcggcgtc ggcgtcacca
     1141 ttgccgaaaa agccccgaag atctgccggg aacgggtgcg ccccatggtg aaaagccacc
     1201 tggtggacgc agtggcggaa tgcctcgcct gcgggctgac cgaaagcgac gtccgcagca
     1261 tcgttgagag cacaatcagc gcgggcacct gtccgtacga agcgcccaaa aaaaagaagt
     1321 aataccggac tgcccggtgc agagtcagcc gcgcgaggcg ggcacaatgt gtaacgtgta
     1381 cgcctcgcgc gttttttcta cgcggcaggg tagcccaaac gcctgggata gcacggacgc
     1441 gcacaggatc tcttcccggg acccttgcgc gagtactgca ccgtccttta gaaccagcac
     1501 gttatcgatc cagtccccga tctcttcgat atggtgcgtg acatagataa cggtgggcgc
     1561 atcgggtgat ccggccagag ccgcaagatc ttcgagcaac cgccagcgcg cggcgggatc
     1621 cagacccacg cagggttcgt ccagcaccag cagccgtgga ttggcgataa gcgcccgggc
     1681 gatgagcacc cgctggcatt ccccctgcga cagcgtcgca taggtctggc cggccagtat
     1741 tgacccgttc acgcgctcga gcgcgccatg cgcgcgcgcg cgttccaaat ccgagaaggc
     1801 gcggtacaca ccgagggacg cgtccaaacc cgagctgacg acatccaggg ccgtgtcgtc
     1861 ttcggggagg cgggattcaa tggcgctgct gacccagccg accattttgc gcagctcggg
     1921 gatattgcat tcgccaaagc gctggcccag cacatgcaca ctcccccggc tcggccattc
     1981 gtagcccgta ataattttca aaagcgtggt cttgcccgcg ccattggctc cgagcagagc
     2041 ccagtggctg ccgtgcccga tacgccacga gacgccgcgc aaaatggcga cccctttgcg
     2101 gtcaaatcca ataccgtcga catgtacaat gctcatggca tcacctgctc ttctgacgtt
     2161 tccaaggttt cgtcgcgggg gtcagacaga ccagccttcc cggcccacca gcgggacaaa
     2221 catacagcgg atgctctcga actcactaaa ccggtcttca ggcccgcgca cgagcacttt
     2281 gagttgctga agttcccggc ttcccacggg gcacacgaga cgccccccgg gcgccagctg
     2341 cgattgcaat gacggcggca ccgcgggtgc ggcagccgtc acgatgatgc catcgtacgg
     2401 agcacgctcc ggccacccga gcgttccgtc gccgatgaca acagacacgt tgctgtattc
     2461 gagcgattcg agcgctttcc gcgcatcttc ggcaagatgg cggaagcgtt cgaccgaaat
     2521 gacctccgcg gccagctcgg ccagaaccgc ggcctggtag cccgaaccgg tcccaatttc
     2581 aagcacccgg ctgtgcggcg tcaattccag caactcagtc atcttcgcga cgatataggg
     2641 ctgcgaaatg gtctggccgg aaccaatttc aaggggacga tcctcgtacg cgcagctcgc
     2701 tttgcccacc ggcacaaatt cgtgccgggg caccgtgcgc atcgccgcga gcacacgcgg
     2761 atccgtgacg ccgcgtccgg caatgtgatg ccgcaccatc gcgtcgcgtt cttcggcata
     2821 ggcgtcggag ggtagcatgc ctgggttgcc ctacagggtc cgcgtagcgc cgcgttccat
     2881 ggcgaacagc agcatcgcga tgctgcgcgc cagccatacc ataaaaaggg cggccagtgg
     2941 gccaaagtca acgggaccca taggaggcag gatccgccgg acgcgctgaa tcaggggctc
     3001 cgtcgcgcgg cctatccagg ccagccgccc ggcatccgta tctacctcaa gccacggacc
     3061 aaaccaccgg agaagtatca tcatcatata cagcgtc
//
LOCUS       DAOPBQ010000463         3083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_25146_length_3083_cov_3.816050, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000463 DAOPBQ010000000
VERSION     DAOPBQ010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25146_length_3083_cov_3.816050"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKW60_12870"
     CDS             complement(<1..571)
                     /locus_tag="PKW60_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPK00372.1"
                     /translation="MIEIAGFERLSTVDWPGCVSAVVFTPGCSLNCAYCHNRELLTRG
                     NSPRIPVESVLATLRTRRGFLDGLVISGGEPTLQEGLESFIREVRSMGFAVKLDTNGT
                     RPRVLKRLLSEGLLDYVAMDAKAPYGLYEDVCGGPVDLDAVHESVRCLVKSSTPFEFR
                     TTVLPYFRPRDIADIGAAIHGAPRYVLQQF"
     gene            complement(571..2718)
                     /locus_tag="PKW60_12875"
     CDS             complement(571..2718)
                     /locus_tag="PKW60_12875"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPK00373.1"
                     /translation="MFDRIRKRDGRIEKFQPEKITWAIFKAASACGGQDFGRAEALAV
                     EVLELAKTRFNGDVPDVESVQDLVEKVLIENGHARTAKAYILYREKRAGARNLNALIG
                     ATIQMFSDYLGDRDWRIKENANMQKSINGLNNYVREFFTKNYWLHEVYPAEVREAHES
                     GDAHIHDLGWLGTYCAGWDLRQLLMMGFGGVPGKVESRPAKHLRSFLGQIVNSTFTTQ
                     GESAGAQAWSSFDTYCAPFIRYDNMTYAQVKQCLQEFIFNINVPTRVGFQCPFSNLTF
                     DLTVPKTLKDQAVIIGGELQEDTYGDFQAEMDLLNQAFCEVMEEGDAKGRVFTFPIPT
                     INITQDFDWDSPSVDAFMRITAKYGIPYFANYINSDLSPEDAVSMCCRLRIDTSQLRL
                     RGGGLFGSNPLTGSVGVFTINLPRVGYLSSTVEEFKMRLWRLVQIGKTSLEIKRKIVE
                     QQTEQGLYPYSAHYLKTVKERTGEYWHNHFSTIGILGMNEALLNFMDTDIVSPEGQQF
                     AADILNYLRELLVEIQNETGHVYNLEATPAEGTAYRLARLDKARYPDIITAGNGTPYY
                     TNSSQLPVGFTDDLFEALELQDELQSMYTGGTVLHAYLGESIEDIDVCKRLVRRAFER
                     FKLPYLSLTPTFSICQDHGYIRGEHFRCPDCGAETEVWSRVTGYLRPVENFNEGKKQE
                     YFDRRKFKVPGEAFEEREAVPAGAAAGEVEHVA"
     gene            complement(2888..>3083)
                     /locus_tag="PKW60_12880"
     CDS             complement(2888..>3083)
                     /locus_tag="PKW60_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00374.1"
                     /translation="YAVAAAYAPCSAICSFINYVKQKRGGWWRFFGMNYAKNINDSAA
                     PALMERTNRLILFGGTPLKR"
BASE COUNT          603 a          934 c          898 g          648 t
ORIGIN      
        1 gaaactgctg gagcacatag cgtggggcgc cgtggatggc ggccccgatg tcggcgatgt
       61 cccgcggccg gaaatacggg agcacggtgg tacggaactc gaacggcgtc gaacttttca
      121 ccagacaccg cacgctctcg tgcacggcgt ccagatcgac cggcccgccg cagacgtcct
      181 cgtacagccc gtaaggcgct ttggcgtcca tggcgacgta atcgaggagc ccctcactca
      241 gcaggcgttt gagtaccctg ggccgggtgc cgttggtatc gagtttgacg gcaaagccca
      301 tgctgcggac ttcacggatg aaactttcaa gcccttcctg cagggtgggt tcgccgcccg
      361 agatgaccag accatcgagg aacccccggc gcgtccgcag cgtggccaga acactctcca
      421 cggggatgcg ggggctgttc ccgcgggtaa gcagttcgcg gttgtggcaa taggcgcaat
      481 tcaaagagca gccgggcgta aacacaacgg ccgagacgca acccggccaa tccaccgtag
      541 agagtctctc gaacccggcg atctctatca tcacgcgacg tgctccactt cgcctgccgc
      601 agctccggca ggcaccgcct ctctctcttc gaaggcttct cccggcacct tgaatttacg
      661 ccgatcgaag tactcctgct ttttgccttc gttgaagttt tcaacggggc gcaggtagcc
      721 cgttacgcgc gaccagactt cggtttcggc gccgcaatcc gggcagcgga agtgctcgcc
      781 ccggatgtat ccatgatcct ggcagatgct gaaggtcggg gtaagcgaga ggtaaggcag
      841 tttgaaccgc tcaaaggccc gccgaaccag ccgtttgcat acgtcgatat cttcgatgct
      901 ctcgccgaga taggcgtgca gcaccgtgcc gccggtatac atcgactgca attcgtcctg
      961 aagctccagg gcctcgaaaa ggtcgtcggt gaatcccacg ggcaactggc tcgagttggt
     1021 gtaatacgga gtgccgttgc cggcggtgat gatgtccggg taccgggcct tgtcgaggcg
     1081 ggcgaggcgg tacgccgtgc cttcggccgg cgtcgcttcg aggttgtaga cgtggcccgt
     1141 ctcgttctga atctcgacga gcagctcgcg caggtaattg aggatgtcgg cagcgaattg
     1201 ctggccttcc ggcgacacga tgtccgtgtc catgaaattc agcagcgcct cgttcattcc
     1261 caggatgccg atcgtgctga aatgattgtg ccagtattcg cccgtgcgtt ccttcaccgt
     1321 cttgaggtaa tgcgcggaat agggatacag cccctgctcg gtctgctgct cgacgatttt
     1381 gcgtttaatc tcgagcgagg tcttcccgat ctgcaccagc cgccaaagcc gcatcttgaa
     1441 ttcttcgacc gtcgacgaca ggtagcccac gcgcggcagg ttaatcgtga acacgcccac
     1501 cgagcccgtc aagggattac tcccgaaaag gccgccgccg cgcaggcgca gctgagacgt
     1561 gtcaatacgc agccggcagc acatggatac cgcatcttcc ggcgacagat cggagttgat
     1621 gtaattggcg aagtatggaa tgccgtattt ggcggtaatg cgcatgaacg cgtcgacgga
     1681 cggcgagtcc cagtcaaaat cctgcgtgat gttgatggtg ggaatcggga aggtgaacac
     1741 gcggcctttg gcgtcgccct cctccatcac ttcgcagaag gcctgattga gcaggtccat
     1801 ctcagcctgg aaatcgccgt aggtgtcctc ctgaagctcc ccgccaatga tgacggcctg
     1861 atctttgagg gttttgggaa cagtgagatc gaaggtgagg ttggaaaaag ggcactgaaa
     1921 cccgacccgt gtgggcacgt tgatgttgaa gatgaactcc tgcaggcact gcttcacctg
     1981 agcataggtc atgttgtcgt aacggatgaa cggggcgcaa taggtgtcga agctcgacca
     2041 ggcctgagcg cctgccgact ctccctgggt ggtgaaggtc gaattgacga tctgacccag
     2101 aaacgagcgc agatgttttg ccgggcggct ttcgaccttg ccgggcaccc ccccgaatcc
     2161 catcatgagg agctgccgca ggtcccatcc cgcgcaatag gttcccagcc agcccaggtc
     2221 gtggatgtgc gcatctccgc tctcgtgagc ctcgcgcact tcggccggat agacctcatg
     2281 cagccagtag ttctttgtga aaaactcgcg aacgtaatta ttgaggccgt tgatgctctt
     2341 ctgcatgttg gcgttctctt tgatacgcca atcccggtcg cccaggtaat ccgagaacat
     2401 ctggatggtc gcgccaatca aggcattcag gttgcgtgcc ccggcccgct tttcgcggta
     2461 gagaatgtag gccttggccg tgcgcgcatg accattttca atgagcacct tctcgacgag
     2521 atcctgaacg ctctcgacat ccggcacatc cccattgaag cgcgtctttg ccagctccag
     2581 aacttctact gccaacgcct cagcacggcc aaaatcctgc cccccgcacg ccgacgccgc
     2641 cttgaaaata gcccaggtga tcttttccgg ttgaaatttc tcgatcctgc cgtcgcgttt
     2701 gcggatccgg tcaaacatgc gcgcttccct ttccccgata tgcggtatga ctgcccaggt
     2761 gtggacgctg ggagaagtca gtgtgccatt agactcgaac cccgtcgagc cggtgcgctt
     2821 tcctcccgac tttactgccc ctccggccac cgcggctaga cgcctcatcg gcagccggtt
     2881 tccctcatca tctcttcagg ggggttcccc cgaacaagat caagcgattg gtgcgctcca
     2941 tcagcgccgg agcggcggag tcgtttatgt ttttcgcata attcataccg aaaaatcgcc
     3001 accagccgcc ccgtttttgc tttacataat tgataaaact gcagatggcc gagcatggtg
     3061 cgtaagctgc cgctaccgca tac
//
LOCUS       DAOPBQ010000464         3072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_25243_length_3072_cov_3.569108, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000464 DAOPBQ010000000
VERSION     DAOPBQ010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25243_length_3072_cov_3.569108"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="PKW60_12885"
     CDS             complement(<1..1334)
                     /locus_tag="PKW60_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPK00375.1"
                     /translation="MENAAQGSLQRPDFFMIAGYFVLMLAIGVYFYRYMKRMKDYFSG
                     GSAIPWWLSGVSFYMTSFSVAAFVFYPSICYRYGWVGVTLLWVAVPATLFSVLLFAKR
                     WRRARIDSPVEYLETRYNPLLRQIFAWQGIPVRMIDDGIKLFATGKFISICTGVDINY
                     SILGAGGIMLIYTFMGGLWAVMVTDFVQFVVLTAGLLVILPLSLQQAGGLSQVFTNSP
                     EGFFRMTSEEYSWTYVIPLIMLYSLAWSSINWALIQRYYCVPKERDALKVGWMVVALY
                     IIGPPLMFFPAIAARQFIPTLQDAGDIYPILCAKLLPAGMLGLAIAAMFAATMSTLSG
                     DYNACAGVLTNDVYRRLFRPKASQKELVAVGRLMTLLVGVIALGTALVLARGRAESMF
                     RIMVTLFGVATAPVAVPMLLGLLSRRYTGRSAVIGFLCGTVVGLLLFALSFH"
     gene            complement(1434..2315)
                     /locus_tag="PKW60_12890"
     CDS             complement(1434..2315)
                     /locus_tag="PKW60_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPK00376.1"
                     /translation="MRMKEAACLAAALILAVTAFACAHAKQCALSTGKSCPAGTTNRL
                     ACRLANYGNYQNIALPHLQHLGVRYVFMNVPEPGAAEQEMAALSQYGLKPLVMRASTD
                     LSKESSVQELGQQAAVCEKMGVKFMFLSAKRNDAPKEAVYDRLRRAGDLAAKHGVTIA
                     LETHPDLGTNADVHLETMKAINHPNIRVNFDSANITYYNKNTDAVTELRKIIDYVATV
                     EIKDHTGEFETWNFPAVGQGVVDVPDLLALLREHGYTGPITLEFEGIKGVELSELETK
                     LAIAESIAYIRSLGNFD"
     gene            complement(2349..>3072)
                     /locus_tag="PKW60_12895"
     CDS             complement(2349..>3072)
                     /locus_tag="PKW60_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPK00377.1"
                     /translation="EDSAHAHCAKLKGKYMGTWSRMSIYSFQMSKPLPAIEGGMGMYQ
                     NKDDFERATAFGNYEMPGVDPESPVYKYRGSGFGLKLRMHPFAAELARCQMRGLEERN
                     AAGARQTRRLNDALIQLPGLYEQSSGRDDIERLYYAWNMLFIDEAEAGMSRDAAVKAL
                     QAEGVNVSGLSYTLQHKLQLYAEDQWWHHKPVIPELPGSEQANATSVALPYFTNEVPE
                     LVDQYIKAFEKVWAHRSAIGRA"
BASE COUNT          625 a          962 c          890 g          595 t
ORIGIN      
        1 ttgtgaaatg acagcgcgaa caggagcaga ccaaccaccg tgccgcaaag gaagccaatg
       61 actgcgctgc ggccggtgta acggcgcgac agcaggccca ggagcatcgg aaccgccacg
      121 ggcgcggtcg ccacgccaaa cagggtcacc ataatgcgga acatgctctc ggcccgcccg
      181 cgcgcgagga ccagcgcggt gcccagcgcg atcacgccta ccagaagcgt catcagacgc
      241 cccaccgcca ccagttcttt ctgcgacgcc ttcggccgga acaggcgccg gtatacgtcg
      301 ttggtgagga ccccggcaca ggcattgtag tcgccgctca gggtggacat ggtcgcggca
      361 aacatggccg cgatggccag ccccaacatg cccgcgggaa gaagcttcgc gcagagaatc
      421 gggtagatgt cgcccgcatc ctggagcgtg ggaataaact gcctcgctgc aatcgcgggg
      481 aagaacatca gcggcgggcc tatgatatag agcgccacga ccatccagcc cactttaagc
      541 gcgtcccgtt cttttggcac gcaataatac cgctggatga gcgcccagtt gatgctgctc
      601 caggcaaggc tgtacagcat gatcagcggg atgacatagg tccagctgta ttcctcggag
      661 gtcatgcgga agaaaccttc gggcgaattg gtaaagacct ggctcaggcc gcccgcctgt
      721 tgaagcgata atggcaaaat gacaagcaaa ccggcggtca gcacgacaaa ttgtacgaaa
      781 tcggtcacca tcacggccca cagaccgccc atgaacgtgt aaatcagcat gatcccgccc
      841 gcgcccagga tgctgtagtt gatgtcgacg cctgtgcaga tggaaatgaa cttacccgtc
      901 gcgaacaact tgatgccgtc atcgatcatg cgcaccggga tcccctgcca ggcaaaaatc
      961 tgccgaagca gggggttata gcgcgtttcc agatattcca ccgggctgtc aatgcgggcg
     1021 cgccgccacc gcttcgcaaa aagcagcacg ctgaacagcg tggccggcac cgccacccag
     1081 agcagcgtca ctcccaccca gccataccgg taacaaatcg acgggtagaa cacaaaggcc
     1141 gcgacactga agctggtcat gtaaaacgag acaccactca gccaccaggg gattgcgctg
     1201 ccaccactga aatagtcctt catgcgcttc atgtagcggt agaaatagac gccgatagcg
     1261 agcatcagaa caaaataccc cgcaatcatg aaaaaatccg ggcgctgcag actcccctgc
     1321 gcggcgtttt ccatcgaggt cattctccag ggtgcgtgtt gaaacgcggc ggccctcacc
     1381 cccgggccgc cgcgtctgat actgtacctc cggcaaaacg ggctgggcgc cggctagtcg
     1441 aagttgccca gcgaccggat atacgcgatt gactcggcga tggcgagctt cgtctcgagc
     1501 tcgctcagct cgacgccctt gatcccttcg aactcaagcg tgatcgggcc cgtatacccg
     1561 tgctctcgaa gcaaagcgag caggtcaggc acgtccacca caccctggcc cacggcgggg
     1621 aaattccacg tctcgaattc ccccgtatgg tctttgatct ccaccgtggc cacgtaatcg
     1681 atgatcttgc gcaattccgt caccgcatcc gtattcttgt tgtaatacgt gatgtttgcg
     1741 gagtcgaaat tgacgcggat attcgggtgg ttaatggcct tcatggtctc aagatggaca
     1801 tcggcgttcg tgccgagatc cgggtgtgtt tcgagcgcaa tggtcacgcc atgcttcgca
     1861 gcgaggtcgc cggcgcggcg aaggcgatcg tagaccgcct ccttgggggc gtcgttgcgt
     1921 ttcgccgaca aaaacatgaa cttcacaccc atcttctcac agacggcggc ctgctgcccg
     1981 agttcttgca cgctggactc tttcgacagg tcggtgctgg cacgcataac caggggtttc
     2041 aggccgtatt ggctgagggc ggccatctcc tgctctgctg ctccaggttc cggcacgttc
     2101 atgaacacgt aacggactcc cagatgctgc agatgaggca gcgcgatgtt ctgatagttg
     2161 ccgtagttgg ccaggcggca tgccagccgg ttggtcgtgc ctgcagggca cgacttaccc
     2221 gtgctgagag cgcattgctt cgcatgagcg catgcaaacg ctgtcaccgc cagtatcagt
     2281 gccgcggcca ggcacgccgc ttccttcata cgcattgcca gtctcctccc gctcgtattg
     2341 agcttgtctt acgccctgcc gattgcgctg cggtgcgccc agactttctc gaaggccttg
     2401 atgtactgat cgaccagttc cgggacctcg ttagtgaagt acgggagcgc aacgctggtc
     2461 gcgttcgcct gctccgagcc cggcaactcc ggaatgacgg gtttgtgatg ccaccactgg
     2521 tcttcagcat acagctgcag cttgtgctgg agcgtgtagc tcaatccgct gacgttcacg
     2581 ccttcggcct gaagggcttt caccgccgcg tcgcgagaca tgcccgcttc cgcttcatca
     2641 atgaacagca tgttccaggc gtagtagaga cgctcgatgt cgtcacgccc gctcgactgc
     2701 tcgtacaggc ccgggagctg gatcagcgca tcgttcaggc ggcgcgtctg ccgtgcgccc
     2761 gcggcattgc gctcttcaag gccgcgcatc tggcagcgtg caagctccgc cgcgaagggg
     2821 tgcatccgca gtttcaggcc gaaccctgat ccccggtatt tatagacggg actctcggga
     2881 tcgacgcccg gcatttcgta gttcccgaac gccgtggcgc gctcaaaatc gtccttgttc
     2941 tggtacatgc ccatgccgcc ttcgatcgcc ggcagcggtt tgctcatctg gaagctgtag
     3001 atggacatac ggctccaggt gcccatgtat ttgcctttca gcttcgcgca gtgcgcatgc
     3061 gcggagtctt cg
//
LOCUS       DAOPBQ010000465         3065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_25310_length_3065_cov_3.771761, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000465 DAOPBQ010000000
VERSION     DAOPBQ010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25310_length_3065_cov_3.771761"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PKW60_12900"
     CDS             <1..1096
                     /locus_tag="PKW60_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00378.1"
                     /translation="VADIPALMGQAQRGSRTIGSGDRPFDLEKPSPDGAFLEPHGDIL
                     HIHPPWKGGTGATYARLDLSLPDFGSLYFESAVAMDKNAVGRSDGATFTVRAESQGET
                     LEKTIHTAGDTPVPLQLELTPFAGRAITLELSVHPGPENNPSYDWARWHTPRVMQRLH
                     GTGTVELAGGVAYTHAAAGGKVVPVRSGPAGLAVEVPLPGAIYFISEAPAPVNLPLDL
                     AATPLTTAFLDTLGHRLEAPPHATARRSEGEVNGQPVQGLFVHPPDEGMTVLVYSVLL
                     PPAAAVFEASAGLRDGADHSDGAECIVEINGQRIASARVAPGEGLMPLHADLSPWAGQ
                     PAVLALITSSAGSCVCDWVLWSAPVIRETP"
     gene            1093..1878
                     /locus_tag="PKW60_12905"
     CDS             1093..1878
                     /locus_tag="PKW60_12905"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPK00379.1"
                     /translation="MNLTRGEWWNEWTLAVKNLVFPIFCQECGRRLLTDDNGYFCATC
                     WELMPRIERPFCTVCGRPHRAAVGLGTRSNFPCGHCLAAGPVRPVRRTYGVARYDGVM
                     EKAIKLFKFGGKKRLVKPLAELMAAFAEQELECDRYALLVPVPLHPVRERERGFNQAR
                     LLARELAPVFPNAALADVLRRIRPTRTQSTLRDPRERRKNIQGAFAMGAIADLAACHV
                     LLIDDVVTTGCTVGENAQVLLSAGAASVDVLAPALAVLREMPG"
     gene            complement(1911..2099)
                     /locus_tag="PKW60_12910"
     CDS             complement(1911..2099)
                     /locus_tag="PKW60_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00380.1"
                     /translation="MAVFKYSAVVKNREEMTESGTIVARDEENARKKLERLDLVDVRL
                     KRIGGISGLFKSLTADIR"
     gene            2514..>3065
                     /locus_tag="PKW60_12915"
     CDS             2514..>3065
                     /locus_tag="PKW60_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00381.1"
                     /translation="MDTSRSVVVRFFRNRRPELRTLAVFLAVLALFFTSGACGLLYQV
                     VWTRKLVLLFGTAAYAVSTVLSIYFLGLALGSLWGGRLADRSRNPLRLYGVFEVLIGV
                     WALLFIVAISPAEGMIVPALRLFSGQRIFAVGLRALLSLLFLLVPVVLMGATLPLLAK
                     YVTAVDRTRGLRIGALYSINTFGA"
BASE COUNT          551 a          986 c          921 g          607 t
ORIGIN      
        1 ggtcgcggac attccagcgc tgatggggca ggcccagcgc ggttcgcgga ccatcgggag
       61 cggcgaccgc cccttcgatc tcgaaaagcc ttccccggac ggcgcgtttc tcgaacccca
      121 tggcgatatc ctccatatcc atcccccctg gaaaggcggt accggcgcca cctatgcccg
      181 tctcgacctc tccctgcccg atttcggcag cctgtatttc gaatcggccg tggcaatgga
      241 taaaaacgcg gtaggacgtt ccgatggcgc aacgttcacc gtgcgtgccg aatcgcaggg
      301 agaaacgctc gaaaaaacga tccacaccgc cggggacaca ccggttccgt tgcagcttga
      361 actgacaccg ttcgccggcc gcgcgatcac gctggaactc tccgtgcatc ccgggccaga
      421 aaacaatccc agttacgact gggcccgctg gcataccccg cgagtcatgc agcgcctgca
      481 cggcacgggt actgtcgaac ttgcgggagg cgtcgcatac acccatgcgg cggccggggg
      541 aaaggtcgtc cctgtacgct ccggtccggc cggcctcgcc gtggaggtcc cgctgcccgg
      601 cgccatctat tttattagcg aagcgcccgc gcccgtgaac ctgcccctcg acctggccgc
      661 aacgccgctc accacggcct tcctggatac gctgggccat cgtctggaag cgccgccgca
      721 cgcgacggcc cggcgcagcg aaggcgaggt gaatggacaa cctgtccagg gcctgtttgt
      781 tcatcccccg gacgagggga tgaccgtgct cgtgtattcc gtgctcctgc cgccggcagc
      841 ggccgtgttc gaggcaagcg caggacttcg cgacggggcg gatcactcgg acggagccga
      901 atgcatagtc gagatcaacg gacagcgaat tgccagcgcc agggtggcgc ccggcgaagg
      961 gttgatgccc ctccacgcgg atctgagtcc gtgggcgggc cagcctgccg tgcttgccct
     1021 gatcaccagt tccgccggat cctgtgtctg cgactgggtg ctgtggagcg cacccgtcat
     1081 tcgcgagacg ccatgaacct gacgcggggg gaatggtgga acgagtggac cctcgcggtg
     1141 aagaatctcg tgttccccat tttctgccag gaatgcggac gccggctcct gaccgatgac
     1201 aacggctatt tctgcgccac ctgctgggaa cttatgccgc gcatcgaacg gcccttctgc
     1261 actgtctgcg gccgccccca ccgcgctgcc gtcggcttgg gcacgcgctc aaactttccc
     1321 tgcggacact gcctggccgc gggaccggtg cgcccggtac gccgcaccta tggcgtcgcg
     1381 cgctacgacg gcgtcatgga aaaagccatc aaattgttca aatttggcgg gaaaaaacgc
     1441 ctggtaaagc cgctggcaga gctcatggcc gccttcgcgg agcaggagct ggaatgcgac
     1501 cgctacgcct tgctggtgcc cgtacccctt caccccgtac gcgagcgcga acgcggcttc
     1561 aaccaggccc gccttcttgc gcgcgagctt gccccggtgt ttccaaacgc cgccctggcc
     1621 gacgttctcc gccgaatccg gcccacgcgt acccagagta ctctgcgcga tccgcgcgaa
     1681 cggcgcaaaa acatccaggg ggcattcgcc atgggcgcca ttgccgatct ggccgcatgc
     1741 catgtgttgc tcatcgacga tgtcgtgact acgggatgca cggtgggcga gaatgcgcag
     1801 gttctactga gcgccggagc cgcctcggtg gatgtgctcg cacccgctct cgcggtgctt
     1861 cgcgaaatgc cgggataact gccccgccgc gcaggcaagg gcgatgctcg ttaccgaatg
     1921 tctgccgtca aggatttgaa caggcccgaa atgccgccaa tgcgtttgag ccggacgtcg
     1981 acgagatcca gacgttccag tttctttcgt gcgttctctt cgtcgcgggc gacaatggtg
     2041 cctgattccg tcatctcttc acggtttttc actacggctg agtatttgaa tacagccacg
     2101 ctaatttctc ctggttctct tacagatgga tgcgccttcc tctacgagtc acccggatga
     2161 aataaaaacg taacgaaact tgtcgccaac gggccgtcgg caccactgcc cactgctaca
     2221 aagataaatg tgtaagaaaa ggtttaatac cgaaaataat gctagcaaag tctccgaaga
     2281 atgtcaagcc ttcacaggaa ataccctgca aaatccacag gagatacgac ttacacaaga
     2341 cgttcggcat gcctcgatcc cggaattggc cggggcagca ccgcccagac tctgaaatca
     2401 ttgtctcccg cgccggggtc tgcgttcacg cccacgtact ttgacacatt gccgggccgt
     2461 cggctagcat caccgcagat ttcattcaaa cgagtaatcg agggacagca atcatggata
     2521 cttcgcgcag tgtcgtcgtg cgttttttcc ggaaccgccg gcccgaatta cgcacattgg
     2581 ccgtatttct ggcggtactt gctcttttct tcacctcggg cgcctgcggg ctgctttacc
     2641 aggtggtctg gacgcgcaag ctggtgctct tgtttggaac ggctgcctat gccgtgagca
     2701 cggtgctatc aatttacttc ctcggtctgg cgctgggcag cctgtggggg gggcgcctcg
     2761 cggaccgcag tagaaatccg ctgcgcctct acggcgtgtt tgaagtgctc atcggcgtgt
     2821 gggccctttt gttcatcgtg gcgatcagcc cggcggaagg gatgatcgtg ccggccttgc
     2881 gcctgttttc ggggcaacgg atattcgcgg tcgggttacg ggcgctgctg tcgctgctct
     2941 tcctgctggt gccagtggtg ctgatggggg ccacgctgcc gttgctggcc aagtacgtta
     3001 ccgccgtcga ccgcacccgc ggactgcgga ttggcgcctt gtacagcatc aacacgttcg
     3061 gagcc
//
LOCUS       DAOPBQ010000466         3061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_25359_length_3061_cov_3.505655, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000466 DAOPBQ010000000
VERSION     DAOPBQ010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25359_length_3061_cov_3.505655"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..588)
                     /locus_tag="PKW60_12920"
     CDS             complement(52..588)
                     /locus_tag="PKW60_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00382.1"
                     /translation="MNYSKSPFNAWTIPMLALWLIFFAVGLFPETVYWTARLAGAVVT
                     QNALINSPSLVTLFLAFYLAFFAYQCCLDAGLSRDSAVLRAIQVGILGLIAFLPYPFY
                     LLLAPGNLEQLNDRLQRADEVVAVFGLPVAKLLAWLYLLITVVRHHLTGGGDIFARMY
                     PRFARQNNGGRLETGPDD"
     gene            complement(858..2738)
                     /gene="mnmG"
                     /locus_tag="PKW60_12925"
     CDS             complement(858..2738)
                     /gene="mnmG"
                     /locus_tag="PKW60_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010900185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPK00383.1"
                     /translation="MSLRDFDVIVVGGGHAGVEAALACARTGHRTLLATMQLDSIAKM
                     SCNPAIGGTAKGQVVREIDALGGEMARCIDRTGIHYKMLNLSKGPAVHSPRAQADRTA
                     YQYDMKRVCEEQSNLTLRQALVEAFLIHGDEIAGIRTSLGTEITARAVIVCAGTFLGG
                     RLHFGMVDEPGGRAGDAAAPGLTRSYQELGFDLGRLKTGTCARIHRRSMNTDVMTPSP
                     GDEHPRPFSFSTPIEGFDPNRILCWMTYSNEATQRIIRDNLDRSPLYSGKIEGIGIRY
                     CPSIEDKVVKFPDKDRHHIFLEPEGLNTQEVYVNGMSTSLPESVQLAMLRTVPGLEEA
                     EIIRPGYAVEYDFVFPTQLKPTLETKRVRGLFHAGQINGTSGYEEAAAQGLYAALNVV
                     RWLEGAPPLYIPRSEAYIGVMIDDLVTRGVLEPYRLFTSRAEYRLLLRHDNADVRLAG
                     YGIADDAFKRQVREKELRVQAELNRLKDTLVPVSEELNRMLADAGEATVSGSQPAAQL
                     LRRTRVSLGDVWRFTPPPASLTFEEAEQVEIRCKYEGYFARQERDVERFRSAETRHIP
                     AGLDFSTVPGIPYEARQRFSEVRPVNLGQASRIPGIRSSDIAALLVYLEKLARETLGA
                     EA"
BASE COUNT          644 a          959 c          833 g          625 t
ORIGIN      
        1 acccctgaat cgttgaccgc gttcgaaatc tacgcgacat ccgcccaggt ttcagtcatc
       61 aggtcctgtt tccagccggc ccccgttgtt ctggcgcgca aacctggggt acatgcgggc
      121 gaaaatatcg ccgccaccgg tcaaatgatg gcgaacgacc gtaatgagta aataaagcca
      181 cgccaatagc ttagcaacag gaagaccaaa gacggccacc acttcgtccg cacgctgaag
      241 cctgtcattc aattgctcca ggttgccggg cgccagcagc agataaaacg gataggggag
      301 aaaggcgatc aaacccagaa tgcccacctg aatagcacgc aacaccgcac tatcccgtga
      361 tagcccggcg tccagacaac attgatacgc gaaaaaagcc agataaaatg ccagaaacag
      421 cgttaccagg cttggggagt taattagggc attttgcgtc accacggcac ctgccaatcg
      481 ggcggtccag tacacggttt ccgggaagag ccccaccgca aaaaagatca gccataatgc
      541 cagcatgggt atcgtccatg cgttaaaggg gctttttgag taattcacgc atttcctccc
      601 gggtaaaacg gcagacgctc taacgtagcc gagcgcgcgg cacgattcaa gtcttttccc
      661 cgggcgcggc ctgcgtaaac ccgttgcccc aacaggattt tcgccatcat ttgcccctcc
      721 cgggcggcca cggacagcac acttcccagc ctgccttttc cggatgcgcg ctgtctgaga
      781 cccggattag cgatttttag tatggcgcgc cagaagggat gaacggattt ccccgcgtcc
      841 ctccccgcat tccgggctca agcttccgcg cccagggttt cacgggcgag cttctcaaga
      901 tacaccagta gcgccgcgat atcggacgag cggatgcctg ggatgcggct tgcttgaccc
      961 aggttcacgg gccgtacttc actgaatcgc tgccgcgcct cgtatggaat gcccggcact
     1021 gtggaaaaat cgagcccggc agggatgtgg cgggtctccg cgctccggaa acgctccacg
     1081 tcacgttcct ggcgcgcaaa atagccttcg tatttgcacc ggatctcaac ctgctccgcc
     1141 tcttcaaagg tcagcgaagc gggcggcggc gtgaagcgcc acacgtcacc aagagatacc
     1201 cgggtccgtc gcaaaagctg cgccgccggt tgcgatcctg agaccgtggc ctcgccggca
     1261 tcggccagca tccgattcaa ctcttccgag acaggcacga gcgtatcttt cagccgattc
     1321 aactccgcct gcacccgcag ttccttttca cggacctgcc gcttgaaggc atcgtcggca
     1381 attccgtaac cggcaaggcg cacatcggca ttgtcgtggc gcagcagcaa acggtactcc
     1441 gcgcgcgaag taaacaggcg gtacggctcg agaacgcccc gcgtgaccag gtcgtctatc
     1501 atcacaccga tatacgcctc gctgcggggg atgtagagcg gcggcgctcc ttcaagccag
     1561 cgaacaacgt tcaaagctgc gtaaagcccc tgcgcggcag cttcctcata tcccgacgtg
     1621 ccgttgatct gccccgcatg aaacagaccc cgcacgcgct ttgtctccag ggtcggtttg
     1681 agttgtgtcg gaaatacaaa atcgtattcg accgcgtaac ccggccggat aatttccgct
     1741 tcttcaaggc cggggacggt gcgcagcatc gcaagctgta cgctttcagg caggctggtg
     1801 gacatgccgt tgacgtacac ttcctgcgtg ttcaacccct ccggttccaa aaagatgtgg
     1861 tgccggtctt tgtcggggaa tttcacgacc ttgtcttcaa tgctcgggca gtagcgtatg
     1921 ccgatgcctt cgatttttcc cgaatacagt ggagatcgat cgagattatc gcggatgatc
     1981 cgctgggtcg cctcattcga ataagtcatc cagcacagga ttcggttcgg atcaaatcct
     2041 tcgatgggcg tcgaaaagga aaacgggcgc gggtgttcgt cgccgggcga cggcgtcatg
     2101 acgtccgtat tcatgctgcg gcgatggatg cgcgcgcagg tccccgtctt caaacggccc
     2161 aggtcaaacc ccagctcttg atacgagcgc gtcaaaccgg gcgctgcggc atctcccgct
     2221 cgccccccgg gctcgtcaac catcccgaaa tgcagccttc ctcccaggaa ggtgcccgcg
     2281 cagacgatca ccgcccgcgc cgtaatctcc gtgcccagac tcgtccgaat ccccgcgatt
     2341 tcgtccccat ggatcaaaaa cgcttcgacc agggcctggc gcagagtcag gttcgattgc
     2401 tcctcgcata cccgcttcat atcgtattgg taggccgtgc ggtcggcttg tgctctcggc
     2461 gaatggactg ccggaccttt agatagattc agcatcttgt aatggatgcc ggtccggtcg
     2521 atgcatcggg ccatctcgcc gcccagcgcg tcgatttcac gaaccacctg gcctttggcg
     2581 gtgcccccaa tggcgggatt gcacgacatc tttgcgatgc tgtcgagctg catggtcgcc
     2641 aacagcgtgc gatgccccgt ccgcgcacac gccagcgccg cttcgacgcc cgcatgtccg
     2701 ccacccacaa caatcacatc aaaatcacgc aagctcatcg gaatgaataa ccgtgtccag
     2761 tgccgaaatt ctgatttcca cgaagaagcc catctatttc atctctttag tgtaccacgg
     2821 caagaaagtg cagggcatgt ttaccccaac cggccggctc tttcgccatt ctggcggaag
     2881 acggtgctca tggcgctgga ggcgtggaag cactgccgtt cgatacccta taatctcgta
     2941 caagcgtgcg attctgcgaa aggagcttct catgcggaaa actgtgatgt gcctgtcgag
     3001 cgttttcctg gctgcctgtg cgttcgggac aacgttttct ttcaatagtc atccggagct
     3061 t
//
LOCUS       DAOPBQ010000467         3056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_25414_length_3056_cov_3.784738, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000467 DAOPBQ010000000
VERSION     DAOPBQ010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25414_length_3056_cov_3.784738"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="PKW60_12930"
     CDS             complement(<1..1304)
                     /locus_tag="PKW60_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00384.1"
                     /translation="MKNPTVRAIIIVLALIWGLIYIYPTLGWMTLRPEERERRLAEWK
                     KEDLEPRQPNFFRDTATGIKRWSQFNKNWVINLGLDLQGGINMIVGFDVSKADPKVLE
                     SFPKEWTKTDIVRHFQEQTLRTIQRRVFEFQSTEPIIARLGDDKIQVQLPGEKDLDRA
                     RDLIFKSAYLTFHLVAMPDEQEQVFLAIDKHFQTTRNANFLSLMQKPISGPYLWVPKE
                     NIEKVKALAEEARQVKGLIPEAMDIAFSGAPQPWDDHQVYTIYLINKTPAITGSGLQQ
                     AMARINDSTAGGSYMILFRFDAASGAQMGQVTEQNIGKPLAIVIDGEVESAPNIQERI
                     TTNGQITGNFSREEAVDLAIALRSGSMPVPLVEESSAQVGASLGRDLIRRGVLSSVLG
                     VIVVVVFMAVYYLWAGIVADIALIMNALLLLAALAYFKSTLTL"
     gene            complement(1359..1622)
                     /gene="yajC"
                     /locus_tag="PKW60_12935"
     CDS             complement(1359..1622)
                     /gene="yajC"
                     /locus_tag="PKW60_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPK00385.1"
                     /translation="MLVAMFAIFYFFLIRPNTKRERERREMLSALSKGDSVVTAGGIH
                     GTIVGLTEKTVVLRVSDDPPVKMEFQRSSVSRVATKDSGSGDE"
     gene            complement(1771..2895)
                     /gene="tgt"
                     /locus_tag="PKW60_12940"
     CDS             complement(1771..2895)
                     /gene="tgt"
                     /locus_tag="PKW60_12940"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPK00386.1"
                     /translation="MNCAVTFEVEARDPHCGARAGRLHTPHGVIETPVFMPVGTQGTV
                     KAMTQDELRSLGAGIILANAYHLYLRPGTEVLREAGGLHRFMGWERAILTDSGGFQLF
                     SLAGLRKITPDGVTFQSHIDGSKHFLSPENAVDLQITIGADIMMCLDECTGYPVEEHE
                     AAESMRMTAAWAARCKQRWLEREAANQALFGIIQGSTFEHLRRESVDRTLALDFPGYA
                     IGGVSVGEGKEEMLRAVEWTAPHLPEEKPRYLMGVGPPEDILDAVERGVDMFDCVMPT
                     RNARNGCLFTSQGRINIKNQKYQRDFGPLDHACHCPVCRGYSRAYLRHLYLAREVLSL
                     RLNTLHNLFFMLHLSAAMREAIRAGRFIEFKASFLQSYRV"
BASE COUNT          576 a          892 c          958 g          630 t
ORIGIN      
        1 agggtcaggg tcgatttgaa ataagccagc gcggccagca gcagcagggc attcatgatc
       61 agcgcaatgt cggccacgat gcccgcccag aggtaataca cggccatgaa caccaccacg
      121 acgatcacgc cgaggaccga cgagagcacg ccccgccgaa tcaggtcgcg gcccaggctc
      181 gcgcctacct gcgcgctcga ttcttcgacg agcggcaccg gcatcgaacc cgagcgcagc
      241 gcgatcgcca ggtccacggc ctcttcacgg ctgaaattac ccgtaatttg cccgttggtg
      301 gtgatgcgct cctggatgtt gggagcggat tcgacttcgc cgtcgataac gatggcgagc
      361 ggtttgccga tgttctgttc ggtgacctgg cccatctgcg cgccactggc ggcatcgaac
      421 cggaacagga tcatatagct gccgccggcg gtgctgtcat tgatccgtgc catggcctgc
      481 tggaggcccg aacccgtaat ggcgggggtt ttgttgatca ggtagatggt gtaaacctga
      541 tggtcgtccc agggttgggg tgcaccgctg aacgcaatgt ccatggcctc ggggatcagc
      601 cccttcacct ggcgggcctc ttcggcgagc gccttgacct tttcaatatt ttccttcgga
      661 acccacaggt aggggcccga aatgggtttc tgcatcaggc tgaggaaatt ggcgttgcgg
      721 gtcgtctgga agtgcttgtc gatcgcgaga aagacctgct cctgctcatc aggcatcgct
      781 acgaggtgaa acgtcagata ggccgatttg aaaatcaggt cgcgggcgcg gtccaggtct
      841 ttttcgcccg ggagctgcac ttgaatcttg tcgtcgccga gacgggcaat gatcggttcg
      901 gtcgactgga attcaaacac acgccgctgg atggtgcgca gggtctgttc ctggaaatgc
      961 cggacgatgt cggttttcgt ccattccttg ggaaagcttt ccaggacttt ggggtcggct
     1021 ttggagacat caaacccgac gatcatgttg atgccgccct gcaagtctag gccgaggttg
     1081 atgacccagt tcttgttgaa ttgcgaccag cgtttgatgc cggtggccgt atcgcggaag
     1141 aagttgggct ggcggggttc gagatcctct tttttccatt ctgcaagccg acgctcgcgc
     1201 tcctcggggc ggagggtcat ccatccgagg gtgggataga tgtaaatcag gccccagatc
     1261 agcgcgagaa cgatgataat ggcgcgcaca gtcgggtttt tcatcggaac gtctccatgg
     1321 gccgcgccat gcgcggcgaa tcagcgcgga tcgcgcggtt attcgtcacc agagccggaa
     1381 tctttggtcg ctacccggga aacggagctc cgctggaact ccattttgac gggtgggtcg
     1441 tcgctgacgc gcaacacgac cgttttttcg gtcaatccga cgatggtccc atggatgcct
     1501 ccggccgtca ccacactatc gcctttagac agggccgaga gcatttcccg gcgctctctt
     1561 tctcgtttgg tgttggggcg gatcaggaag aagtagaaaa tggcgaacat cgccacgagc
     1621 atcagaacct gggggctgcc tagaaaactc ccgccgccgg gtcccggggc tgcctcaccg
     1681 ccactctggg ccgcctgctg gacggcctct gctgcataca acagtctcca cacgacgaac
     1741 aatcctccgt ttcaggaata gaatgccccc tcagacccgg taactctgaa gaaaggaggc
     1801 cttgaactca ataaagcgcc cggcacggat ggcctcacgc atcgcagcgg ataagtgtag
     1861 cataaagaaa aggttgtgta aagtattcag gcggagcgac agcacctccc gcgccaggta
     1921 gagatggcgc aggtacgccc ggctgtaacc ccggcacacc ggacagtggc acgcgtgatc
     1981 gagtggcccg aaatcgcgct ggtatttctg gtttttgatg ttgatccggc cttgtgaagt
     2041 aaacaggcag ccgtttcgtg cgttgcgcgt gggcatgaca caatcgaaca tatccacccc
     2101 gcgttccacc gcgtcgagga tgtcctcggg cggcccgacg cccatcaggt agcgcggttt
     2161 ttcttcgggc agatggggcg cggtccattc cactgcgcgc agcatctctt ctttgccttc
     2221 gccgacactg acgcccccga tcgcgtagcc gggaaaatcc agggcgaggg tgcggtcgac
     2281 gctttcgcgg cgcagatgtt cgaaggtgct gccctggata atgccgaaga gggcctgatt
     2341 cgccgcctca cgttccagcc agcgctgctt gcagcgtgcg gcccacgcgg cggtcatgcg
     2401 catggattcc gcagcctcgt gttcctcgac ggggtatccc gtgcattcat cgagacacat
     2461 catgatatcg gccccgatgg tgatttggag gtcgacggcg ttttcggggc tcaaaaagtg
     2521 tttcgagccg tcgatatggc tttgaaacgt cacgccgtcc ggggtgattt tcctcaatcc
     2581 ggccaggctg aagagctgaa accctcccga atcggtcagg atcgcgcgtt cccagcccat
     2641 gaaccggtgc agcccgcccg cctcacggag gacttccgtg ccggggcgca ggtagaggtg
     2701 gtaggcgttg gcgaggatga tcccggcgcc gaggctgcga agctcgtcct gggtcatggc
     2761 tttgaccgtg ccctgtgtgc cgacgggcat gaacacgggc gtctcgatga ccccgtgcgg
     2821 cgtatgaagg cggcccgccc gcgcaccgca atgcgggtcg cgtgcctcga cctcgaatgt
     2881 gaccgcacag ttcatgatga tggtgaattc ctgtctagtc gccggttttc acgaaatccc
     2941 gctcgagcag acggattttc acgggaggcg cggcctttcc acccggcagg aaggtgtagc
     3001 gggtcgtgac ccacaattcg cccggtgtgc gctagaggat gtaggggtag gccaat
//
LOCUS       DAOPBQ010000468         3055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_25426_length_3055_cov_4.368000, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000468 DAOPBQ010000000
VERSION     DAOPBQ010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25426_length_3055_cov_4.368000"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..695)
                     /locus_tag="PKW60_12945"
     CDS             complement(15..695)
                     /locus_tag="PKW60_12945"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HPK00387.1"
                     /translation="MKALTANQMREADRRCIEDVGIPSVVLMNTAGTRVFEHVNRGPV
                     GIVCGRGNNGGDGFVVARLALLAGFEPRVVVIGELDSLKGDPALFFKAYRNLGGRAVV
                     ARSETDALREVGALRDCAVLVDALLGTGITGEVRGPVRAAIDTWPEVYTIAVDLPSGL
                     DADTGEVLGNCVRADITVTFQYPKLGLVMPEAKRYIGRLVVADIGIPTMCADDEAWFE
                     FERARQGG"
     gene            992..1936
                     /locus_tag="PKW60_12950"
     CDS             992..1936
                     /locus_tag="PKW60_12950"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HPK00388.1"
                     /translation="MTAERFILFIVAPVLLCAGTAPAGDGTAATGADSPRTLSIGGKV
                     LAEYRYTGVPYKPYISVFRTPAGINVLRDAPADHLHHHALMFAIAVDGIDFWQEGNAP
                     GRQVHREFLKSGDASSRQETAQFVERLDWLAPETETPLLHEKRSVRIFDPLHFGASLM
                     TWETELSVPEGRESAALTGSHYFGLGMRFLESMDNDGIFFNADNATGDWVRGDEHVTR
                     SRWCAYTSLADGKPVTVAMFDSPDNPRHPAWWFTMSTPFDYISATLNLYREPMELKAG
                     APLRLRYGVALWDGHVGAAEVEAMYQKWRTAHVSAENQ"
     gene            1990..>3055
                     /locus_tag="PKW60_12955"
     CDS             1990..>3055
                     /locus_tag="PKW60_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00389.1"
                     /translation="MDSSMHMERGSSLSRRKFIGRSASLAAGMAVLNAAPAIAQTTAS
                     ANEKLGVGIIGCGGRGTGHLGTWQWLNGDGGNVEIVALCDVYRPRLNKAAAAAANAKT
                     YMDYRELLADPNVDIVSIATPDHHHGQQAIDAMRAGKDVYCEKPLTHWNQFEVARDIA
                     RTARETGRVFQLGTQGMSDPAWWQMKALVQEGLIGQPLHAECGIFRVGDYGERGMPID
                     DPEARPGPDLNWDAFLGDRPKRDFDASRFFRWRMYEDYAGGPSTDLFPHSFTPVTTML
                     DLKAPAYAVGTGGIYRYSLSETQKIDPGETVEREIPDTFNMLVDYPGGITCALLGSQG
                     NGYQGTGERGSGTKVPVLRGW"
BASE COUNT          627 a          986 c          904 g          538 t
ORIGIN      
        1 acgcatgagg ccgatcatcc accctgccgg gcgcgttcaa actcgaacca ggcctcgtcg
       61 tcggcacaca tcgttggaat gccgatgtcg gccacgacca gccggccgat ataccgtttt
      121 gcctcaggca tgaccagccc cagcttagga tattgaaacg tcacggtaat gtcggcgcga
      181 acgcaattgc ccaggacttc ccccgtgtcg gcatcgaggc cggagggcaa atccaccgca
      241 atggtataga cctcaggcca ggtatcaatt gccgcacgca ccgggccgcg tacctcgcct
      301 gtaatgcccg tgcccaggag ggcgtccacg agcacggcgc aatcgcgcaa agcgcccacc
      361 tcccgcaggg cgtcggtttc actacgcgcc acaacagcac ggccgcccag attgcggtat
      421 gccttgaaga aaagggccgg atcgcctttc agagaatcca gttcgccgat caccacaacg
      481 cgcggctcga acccagccag cagtgcgagg cgcgcgacca caaagccgtc gcctccgttg
      541 ttaccccttc cgcacacgat gcccacgggg ccccggttca catgctcgaa cacgcgcgtg
      601 ccggccgtat tcatcagcac cacgctggga atgcccacgt cttcaataca gcggcgatcg
      661 gcttcgcgca tctgattcgc tgtcaaagcc ttcatcggcg caactccttt tttggcctga
      721 tacgatgctg taccgcgccc cgcgggtgcg aaaaaaagaa ccatccgccg gcttgaatgc
      781 acaaccctgc cgaagtcaaa attcctgcgc ggcatcttac tctagaccat ttgcccgggc
      841 cgggccaaat cgactgcttc actggagctg cgcgcaaccg tgcccgcgct cgagcggcac
      901 gccctggaag atgaagttgc tcgttccttt cctgacgcat gcgatatact gagcgtcggc
      961 gccggaagcc ggtgtccaag ggagaatcgc catgacagcg gagcgtttca ttctcttcat
     1021 tgtggcgccg gtgctgctct gcgccggaac cgcccctgcc ggggacggca cggccgcaac
     1081 cggggcagac tcgccacgaa ccctgagtat tggcggcaaa gtcctcgcag aataccggta
     1141 taccggtgtt ccgtataaac cgtacatctc cgtgttccgc acacccgccg gcatcaacgt
     1201 cctccgcgac gccccagcgg atcacctgca ccaccacgcc ctgatgttcg cgattgccgt
     1261 ggatggcatc gacttctggc aggagggaaa tgcgcccggg cgccaggtac accgtgaatt
     1321 tttgaagtcc ggcgatgcga gcagccgcca ggaaacggca caattcgtcg agcggctcga
     1381 ttggctcgcg cccgaaactg aaacgccgct cctccatgaa aaacgcagcg tgcggatctt
     1441 cgatccgctt cacttcggcg cttccctgat gacgtgggaa accgagttgt ccgtgccgga
     1501 aggcagagaa tccgccgcct tgaccggctc gcactatttc ggtttgggaa tgcgctttct
     1561 ggagtccatg gacaacgacg gcatcttctt caacgccgac aatgccacag gagactgggt
     1621 gcgcggcgat gaacacgtaa cccgctcgcg gtggtgcgcg tatacgtcgc tggccgatgg
     1681 aaaaccggtc acggtcgcca tgttcgattc tccagacaat ccgcgccatc ccgcctggtg
     1741 gtttaccatg agcaccccct tcgactacat ttccgctact ctgaacctgt atcgtgaacc
     1801 catggaactg aaggcaggcg caccgctccg gctgcgatac ggcgtggctc tctgggatgg
     1861 ccatgtcggc gcggcggaag tcgaagcaat gtatcagaaa tggcgaacag cccacgtatc
     1921 ggcagaaaat cagtaagttt gtgaagcgca gagagcacgg gcccgcttcg agcacaggag
     1981 gaacacaacg tggacagcag catgcacatg gagagaggat cttcgttgag ccggcggaaa
     2041 tttatcgggc ggagcgcctc gcttgccgcc ggtatggcag tcctgaatgc ggcgcccgcc
     2101 atcgctcaga caaccgcaag tgccaacgaa aagctgggcg tgggcatcat cgggtgcggc
     2161 ggccgtggca cgggccatct gggcacgtgg caatggctca acggcgacgg cggcaacgtt
     2221 gagattgtgg ccttgtgtga cgtgtaccgg ccgcgcctca acaaggccgc cgcggcagcc
     2281 gccaatgcaa agacctatat ggattaccgg gaattgctcg ctgatccgaa tgtggacatc
     2341 gtcagcatcg caacccccga tcaccaccat ggccagcagg ccatcgacgc catgcgcgcg
     2401 gggaaggatg tctattgcga aaaacccctg acccactgga accagttcga ggtcgcccgg
     2461 gacatcgcgc gcaccgcacg cgaaaccggc cgcgtgtttc agctcggcac ccagggcatg
     2521 tctgacccgg cctggtggca gatgaaggcc ctggttcagg aagggctgat cggtcaaccg
     2581 ctccatgccg aatgcggcat ctttcgcgtg ggcgactatg gcgagcgcgg catgcccatc
     2641 gacgatcccg aggcccggcc tggcccggac ctgaactggg acgccttcct cggagatcgc
     2701 ccaaagcgcg actttgatgc cagccgtttt ttccgctggc gcatgtacga ggactatgcc
     2761 ggcgggccga gcaccgacct cttcccccat tcgtttacgc cggtgaccac gatgctggac
     2821 ctcaaagcgc ccgcgtatgc cgtaggaacg ggaggcatct accggtattc tttgagcgaa
     2881 acacaaaaaa ttgaccccgg cgaaacggtt gagcgcgaga ttcccgacac attcaacatg
     2941 ctggtcgact atcccggcgg catcacctgt gcgctcctgg gatcgcaagg caacggctac
     3001 cagggtacgg gcgagcgggg ttcaggaacc aaggtgccgg tattgcgcgg atggg
//
LOCUS       DAOPBQ010000469         3052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_25460_length_3052_cov_4.249583, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000469 DAOPBQ010000000
VERSION     DAOPBQ010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25460_length_3052_cov_4.249583"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..121)
                     /locus_tag="PKW60_12960"
     tRNA            complement(46..121)
                     /locus_tag="PKW60_12960"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(86..88),aa:Asn,seq:gtt)
     gene            complement(222..2933)
                     /locus_tag="PKW60_12965"
     CDS             complement(222..2933)
                     /locus_tag="PKW60_12965"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HPK00390.1"
                     /translation="MSVEAGIQFPGPEWEHRTPAETGVDGASLDTLAGRLGGRGCVIK
                     DGYVIKDWGDQTRIGDWASAAKPVLSTLLFFAIEEGLVTSVDQPVADFGWALQPRHRG
                     ITFRQLGAMTSGYARPEGAGEAWAYNDFAIQLYQKTLFEKIFKQQSKEAAEQRLAALG
                     LQDGLRFTERNRLSASVRDYARIVWFWTQKGQWDGRQVLPRRYFNEYMRPQTPKDIPL
                     SQETGEDDDYLAIGSYGGGSNHFSNAGPGIYGSNWWFNDTGRFHPEEMTWPDAPPDTV
                     MAMGVRGNCAAFIPSLNAALICADGKWGDIEGGNRDARLNQILGLFARANGYTPLDSS
                     APSHAKWSRVVLDFEGPAANAADSDPNPFLDYRMQVRFTGPGGSEYDVPGFFDGDGAG
                     GLEGSTWRVIFSPDAVGEWRYRVAFRKGAGLAVSLDPDEGEPAALDGTTGGFLVRPQT
                     DAAQGFYRLGRLEYVGAHYLKLRDDGYWLKGGSNSPEDFLAYEGFVNTPYASHTYQTH
                     VADWQPGDPDWDNGKGKGIIGALNYLASVNVNSIYFLPMNIGGDGKNAYPYLGPIDRE
                     GSAANDNLHFDLAKLRQWDLVFDHAQRKGIMLHFVLNEAEELNKEELDDGLLGVERKL
                     YYRELVARFACFPALQWNLCEEYNIKHPLAPKLVKDFAEYLGNVDPYDHPITVHHADT
                     VDKGWTPFLGDPRFTVTSFQTNDIGVVRPWREKSRAAGVPLVIGMDEFYPDYSSAKNA
                     DRQRREYLWPVYFSGGQVEFIRDEKFPTGDFRECEALWHYMSNARLFIMEHLPFWEME
                     PANELLTGAAVFQGKYNRIPGHVFAKPGECYAVYLPIAQPTGTLDLTDAAGEFIQRWY
                     DPRAGVFAGNETTVTGGSLLRLGPAPEKPGRDWVVLITRAEPGDSPP"
BASE COUNT          586 a          847 c          981 g          638 t
ORIGIN      
        1 ggtcttgcgt taaacaagaa ccgctgaaat cagcggattg aattttggct ccgcggacag
       61 gactcgaacc tgtgacctag tggttaacag ccacccgctc taccgactga gctaccgcgg
      121 aacaggcacc tgaatggttt cggaatttta ctatatgcat gctgcgcggt caaattattg
      181 ccggaccttt aatgcggcat gaatgcggcg tgggggctcc atcacggcgg ggaatcgccg
      241 ggttcggccc gcgtaatgag gacgacccag tctctcccgg gtttttcggg cgcggggcca
      301 agccgcagga ggctgccccc ggttacggtt gtctcgtttc cggcgaatac gccggcgcgg
      361 ggatcgtacc agcgctggat gaattcaccc gcagcgtccg tgagatcgag ggttcctgtc
      421 ggctgggcaa tgggcaggta gacggcatag cattcgccgg gtttggcgaa gacatgtccc
      481 ggtatgcggt tgtatttgcc ctggaacaca gccgcgccgg tcaggagttc gttggcgggc
      541 tccatctccc agaagggcag gtgctccatg atgaacaggc gggcattgct catgtaatgc
      601 cagagcgctt cacattcccg gaaatcgccg gtggggaatt tttcatcgcg gataaattcg
      661 acttgccccc cggagaaata gaccggccag aggtattccc gccgctgtcg atcggcgttt
      721 tttgcgctgg aatagtcggg atagaattcg tccataccaa tgaccagagg gaccccagcg
      781 gcgcgggatt tctcgcgcca tggcctgacg acgccgatgt cgttggtctg gaaagacgtg
      841 acggtgaaac ggggatcgcc gaggaagggc gtccagcctt tgtcgacggt gtcggcgtga
      901 tgcacggtga tggggtggtc gtaggggtcg acattcccca ggtattccgc aaaatctttg
      961 accagtttgg gggccagtgg gtgcttgatg ttgtattcct cgcagaggtt ccactggagc
     1021 gcggggaagc aggcgaaccg ggcgacaagc tcgcggtagt agagtttgcg ttcgacgccg
     1081 agaaggccgt cgtcgagctc ttctttattc agttcttccg cttcgttgag cacgaagtgg
     1141 agcatgatgc ccttgcgctg ggcgtggtca aacacgagat cccactggcg gagtttggcc
     1201 aggtcaaaat ggaggttgtc gttggcggca ctgccctcgc ggtcaatggg gccgaggtag
     1261 gggtatgcgt tttttccgtc gcccccgatg ttcatgggca ggaagtagat gctgttcacg
     1321 ttgactgagg caaggtaatt gagcgcgccg atgattcctt ttcctttgcc attgtcccag
     1381 tcgggatcgc cgggttgcca gtcggccaca tgggtttgat aggtatggct ggcatagggc
     1441 gtgttcacaa atccctcata ggcgaggaag tcctcggggc tgttagagcc gcccttgagc
     1501 cagtacccat cgtcacgcag cttcagataa tgggcgccga catattccag ccgtcccaat
     1561 cggtaaaacc cttgtgcggc gtccgtttga ggccggacga gaaatccgcc cgttgtgccg
     1621 tcgagtgcgg ccggttcacc ttcatcggga tcgaggctga cagcgaggcc ggcccctttc
     1681 ctgaacgcga cgcggtatcg ccactcgccg acagcgtccg gggagaagat cacgcgccag
     1741 gtgctccctt cgaggccgcc cgcgccgtcg ccatcgaaaa agccgggtac atcgtattcg
     1801 ctgcccccgg gccccgtgaa gcgcacctgc atgcggtaat ccaggaacgg gtttgggtcg
     1861 gaatccgcgg cgttcgcggc gggtccttca aaatcgagaa cgacccggct ccatttggca
     1921 tgagacgggg cactggagtc gagcggcgtg tagccgttgg cgcgtgcaaa aaggccgagt
     1981 atctggttga ggcgggcgtc gcggttaccg ccttcaatat cgccccactt gccgtcggcg
     2041 cagatgagtg cagcattcag gctggggatg aaagccgcgc aattccctcg aactcccatg
     2101 gccatgacgg tatccggggg ggcgtccggc caggtcatct cttcgggatg aaaccggccg
     2161 gtatcattaa accaccagtt ggaaccgtag atgccgggcc cggcgttgct gaaatggttg
     2221 gaaccgccgc cgtatgagcc aatggcaagg taatcgtcat cctcgccggt ctcttgggag
     2281 agggggatgt ccttgggcgt ctgagggcgc atgtattcat tgaaataacg cctgggcaaa
     2341 acctgacggc cgtcccactg tcctttttgt gtccagaacc agacgatccg cgcataatcg
     2401 cgcacggatg cgctgagccg gttccgttca gtgaagcgga ggccgtcctg aagtcccaaa
     2461 gcggccagac gctgttcagc ggcttccttg ctctgctgtt tgaagatttt ttcgaaaagc
     2521 gtcttctgat acagctgaat tgcgaaatcg ttatacgccc aggcctcgcc ggcgccttcg
     2581 ggacgggcgt aaccgctggt catggcgccg agctggcgaa acgtgatgcc gcggtggcgc
     2641 ggctggaggg cccagccgaa atctgcaacg ggctgatcca cactcgtcac gaggccttct
     2701 tcaatggcga agaaaaggag ggtgctcagg acgggttttg cggcagaagc ccagtcgcca
     2761 atccgggttt gatcgcccca gtctttgatg acgtatccgt ctttgatcac gcagccgcgg
     2821 ccgccgaggc gtccggccag ggtgtcgagc gatgcaccat cgactcccgt ttcagccggg
     2881 gtacggtgtt cccattccgg gccgggaaac tgaatgccgg cttcgacact cacggcgagg
     2941 gctgcggtac agaaaaaaac agcgagatgg cggcacattg aaacatcctc aagttgttga
     3001 ctgccggggc acacggacac gccaggggct tttcaaaaac gtagattcat aa
//
LOCUS       DAOPBQ010000470         3047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_25507_length_3047_cov_5.005682, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000470 DAOPBQ010000000
VERSION     DAOPBQ010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25507_length_3047_cov_5.005682"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PKW60_12970"
     CDS             <1..671
                     /locus_tag="PKW60_12970"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00391.1"
                     /translation="LGLVIESLIEEYRLDAFALRCWIELQEQLGISPCVLLGDFNQRG
                     IPASCEVDVGNAVAMYALQLASQSPAMCLDWNNNYADDEDKCILFHCGPIPPSLMTGK
                     GRISDHAILANSVGKGRGFGCNTGLIRPGEFTFASMMTHEGKPRFYVGQGEFTSDPIP
                     EDFFGCAGVAQIDRLQDLLLYVGRNGYRHHVSATPGRYAAPVAEALEYYLNMDATLPQ
                     EERG"
     gene            668..1567
                     /locus_tag="PKW60_12975"
     CDS             668..1567
                     /locus_tag="PKW60_12975"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPK00392.1"
                     /translation="MNATAAMVQRAFEQGLVVPAFNVPYLPMIEPVVRAIADCDSVGL
                     IETARLEWYKFEGRGLGPVKAEFDKWARPEVGLHLDHVPVIDEDDQRVDYRAIIEEAL
                     ALGYGSVMVDGSRLSLEGNIQATREIAELAHAAGVPCEAELGAILGHETGPLPPYEEM
                     FASGRGFTRVEDARRFVAESGCDWLSVAIGTIHGAISVALRDQKKVEARLDLDHLDAL
                     RDATGVPLVLHGGSGVKRDYILEGVKRGIAKVNIATEIRQPYEQAWRAHGSIKAAQEA
                     VYARTCWVIRDYFGIEGTRHRLL"
     gene            1608..>3047
                     /locus_tag="PKW60_12980"
     CDS             1608..>3047
                     /locus_tag="PKW60_12980"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPK00393.1"
                     /translation="MSFLGIDVGTTGCKTCVFRDDGALIASAYREYDIHTPRPGYAEL
                     DAHAVWREIKDCIAQVSAASAREPITALSVSSLGEAVVPVSRDRDILGPSLLNFDERG
                     AEFLEDLGRRLDDQQLYRINGNTLGNHYGLTKLLWMKRHCPDIWENAHKFLLWGSFVS
                     CMLGAEPVVDYSLANRTLLFDVEAREWSGELLAWAGLDPAKLPAIAPSGTVIGNVSQG
                     LAEELGLPVNTAIVTGGHDQCCNAAGAGVVKPGDAFLGMGTFLCAAPVFESRRDPEIM
                     LPHGLNTEHHVVPDRFLTFLYNQAGCLLKWFRDTFAAAEHRAAQEAGVDVYPQLLAEM
                     PDAPANVTVLPHFTTTGPPEFVADSSGVMAGLRLETRRGEILRGILEGSVFYLRECLE
                     RARTAGIEVTRMCAVGGGSQSDAWLQLTADILKTPLVRPKQTEAGALGAAMLAAAGTQ
                     AFASLEEAVAAMSAPERDFEPRPAMSSQYE"
BASE COUNT          545 a          982 c          974 g          546 t
ORIGIN      
        1 ggctgggcct ggtcattgaa tcgctgatcg aagagtaccg gctggatgcg ttcgcgctgc
       61 gctgctggat cgagctgcag gagcagctcg gcatctcgcc gtgcgttctg ctgggcgatt
      121 tcaaccagcg aggcatcccc gcttcgtgcg aggttgatgt cggcaacgcc gtggccatgt
      181 acgccctcca gctggcctcc cagtcgccgg ccatgtgcct ggattggaac aacaattacg
      241 ccgatgacga ggacaagtgc atcctcttcc attgcggccc gattccgccc tcgctcatga
      301 ccggcaaagg ccggatcagt gaccacgcca ttctcgccaa ttcagtgggc aaagggcgcg
      361 gtttcggctg caacaccggc ctgatccgcc ccggcgagtt cactttcgca agcatgatga
      421 cccacgaagg taagccgcgg ttctacgtcg ggcagggtga gtttaccagc gatcccatac
      481 ccgaagactt cttcggatgc gcgggtgtag cgcagattga ccggctgcag gatctgctgc
      541 tgtacgtcgg cagaaacggc taccgccatc acgtcagcgc cacgcccggc cggtatgcag
      601 cgccggtggc cgaagccctc gagtattacc tgaacatgga cgcaaccctg ccgcaggagg
      661 agcgcggatg aacgctaccg cggccatggt ccagcgggcc ttcgagcaag gcctggtggt
      721 tcccgcgttc aacgtgccct acctcccgat gatcgagccg gtggtgcggg cgatcgccga
      781 ttgcgacagc gtggggctga tcgaaacggc gcgcctggaa tggtacaaat tcgaaggccg
      841 ggggctgggg cccgtcaaag cggaattcga caaatgggcc cggcccgaag tgggcctcca
      901 tctggaccat gtccccgtga tcgacgaaga cgaccagcgc gttgactacc gggccatcat
      961 cgaggaagcc ctggcgctgg ggtacggttc ggtcatggtc gacgggtcgc gcctttctct
     1021 cgaagggaac attcaggcaa cccgcgagat cgccgagctg gctcacgccg ccggggtccc
     1081 ctgcgaagcg gaactcggcg ccatcctcgg gcacgagacc ggcccgctcc cgccctatga
     1141 ggagatgttc gcctccggcc gtggatttac ccgggtggaa gacgcgcgcc gtttcgtcgc
     1201 cgaatccggc tgcgactggc tttcggtcgc gatcggcacc attcacggcg caatttccgt
     1261 cgcattgcgc gaccagaaaa aggtggaggc ccgcctcgac ctggaccatc tcgacgcgct
     1321 tcgcgacgcc acgggagtgc cgcttgtact gcacgggggg tccggggtaa aacgcgacta
     1381 tatcctcgaa ggggtcaaac ggggcattgc caaagtgaac atcgccactg agatccgcca
     1441 gccatacgag caggcgtggc gtgctcacgg cagcatcaag gcggcccagg aagcggttta
     1501 tgcgcgcacg tgctgggtca ttcgcgatta tttcggcatt gaagggacgc gccacaggct
     1561 cctctgaacg cgccacggcg gcgggccggg acaaccgggg gggatcgatg agctttcttg
     1621 gaatcgatgt cggcacgacg ggctgcaaga cctgcgtctt ccgtgacgat ggcgcgctta
     1681 tcgcgtccgc ctaccgcgaa tatgatatcc acacaccccg gcccggatac gcggaactgg
     1741 atgcgcatgc cgtgtggcgc gagatcaaag actgcatcgc gcaggtgagc gccgcttccg
     1801 cccgcgaacc cattaccgcg ctgagcgtct cttctctggg ggaggcggtg gtgcccgtgt
     1861 cgcgtgaccg ggacatcctg gggccgtcgc tcctgaattt tgacgagcgc ggcgccgagt
     1921 ttctcgaaga cctgggccga cgccttgatg atcaacagct ctaccgcatc aacggcaata
     1981 ccctcggcaa tcattacggc ctgaccaaac tgctgtggat gaagcggcat tgccccgata
     2041 tttgggagaa tgcccataaa tttctcctct gggggagttt cgtttcctgc atgctcgggg
     2101 cggagcccgt ggtggactat tcgctcgcca accggaccct gctctttgat gtcgaggccc
     2161 gggaatggtc cggcgagctc ctcgcctggg ccggcctgga tcccgcaaaa ctccccgcca
     2221 tcgccccgtc aggcaccgtg atcgggaatg tgtcgcaagg cctcgcggag gagctggggc
     2281 ttcccgtaaa cacggcaatc gtcacgggag ggcacgacca gtgctgcaac gcggccggcg
     2341 cgggcgttgt gaaacccgga gacgccttcc tcggcatggg cacgttcctg tgtgcggctc
     2401 cggtctttga atcccggcgc gaccccgaaa tcatgcttcc gcacggattg aacaccgagc
     2461 accacgtggt gccggaccgg tttctcacct ttctgtacaa tcaggccggc tgcctgctga
     2521 aatggttccg cgataccttc gccgccgccg aacaccgtgc ggcccaggag gcaggagtgg
     2581 acgtctatcc ccagctgctc gccgaaatgc cggatgcgcc cgcaaacgtc accgttctgc
     2641 cgcatttcac aaccacgggg cccccggagt ttgtcgctga ttcaagcggc gtgatggccg
     2701 ggctgcggct tgaaacccgg cgcggcgaga ttctgcgggg catccttgaa ggaagcgtct
     2761 tctatctgcg cgaatgcctc gaacgcgccc ggaccgccgg catcgaggtg acaaggatgt
     2821 gcgcggtcgg cggcggcagt caatccgacg cctggctgca actcacggcc gatatcctca
     2881 agacgcccct cgtccggccg aaacaaaccg aagcgggcgc ccttggtgca gccatgcttg
     2941 ctgccgcggg aacgcaggcc tttgcctcgc tggaagaagc cgtcgcggcc atgtccgctc
     3001 ccgagcgcga ttttgagccc cgcccggcga tgtccagcca gtacgaa
//
LOCUS       DAOPBQ010000471         3036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_25628_length_3036_cov_3.785978, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000471 DAOPBQ010000000
VERSION     DAOPBQ010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25628_length_3036_cov_3.785978"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PKW60_12985"
     CDS             complement(<1..1056)
                     /locus_tag="PKW60_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00394.1"
                     /translation="MISRKTSQDGFALLWALLAIMLVLGSVALAVMRVHASGRFTDDA
                     VQKVLLDEACKAGVDIGIARIWNDYVVGNGNTTGNMASYRLYIDELVDNNEDINGNGV
                     RDAGESDLNGNGIFETGAPATIIGSSSARRLRNGATITEVTVSRTDDLTGTSLTLRAT
                     AELGQATQTCQQTIRISGKLFEGFEFAILANNINCILCHAEIHALDLARNTDPARFGT
                     FDRVKVASLESLLYRRTEACSNVAGTVYTRGQIYDTTGAPLSPAQVASSTFKGYSFSN
                     VNGKLIQNSSGAMSKTGLAQAELDPFGTLRPFANLYKDYPTERALMTDGPLPESFPAP
                     FPDDNGNPYKSSGGKMVW"
     gene            complement(1080..1715)
                     /locus_tag="PKW60_12990"
     CDS             complement(1080..1715)
                     /locus_tag="PKW60_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK00395.1"
                     /translation="MRESGFTLVELMIAMSLLAVIGIMSFVAIETAANSSTLALAKDR
                     VQSSVRDTMAAIIAEAEIAARQGDNSLIPPLEEIEVIQNPVPGSPVELRLQVPLDGSR
                     QRWSSPIRFRYMNEDLNRNALLDAGEDTDGDGALTRRIVRIQDINMDGDTADIGEARP
                     VGGCNDLSDVEFALNDDNSILTITLTATARVDGRRPTLVSETVSARVYVCN"
     gene            complement(1726..2169)
                     /locus_tag="PKW60_12995"
     CDS             complement(1726..2169)
                     /locus_tag="PKW60_12995"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPK00396.1"
                     /translation="MAGGNEQMRVGRQGVAASKAGMTLLEIMIAVTIFMVVLGGVAQI
                     LISFYAAQLMQSQRLAVAENAKSVFTEMRQHRDQHPDDFPGAVTTRWPDGSVIPGAGT
                     QTNETIRVDYVNTAANPLEITLTSTWVDLHNRTMTMTVSTVLTDR"
     gene            2857..>3036
                     /locus_tag="PKW60_13000"
     CDS             2857..>3036
                     /locus_tag="PKW60_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPK00397.1"
                     /translation="MGTKGREIVGVDVPQLLEMLNKAFADEWLAYYQYWVGARVASGR
                     MRGIIAHELEEHAGEE"
BASE COUNT          622 a          868 c          860 g          686 t
ORIGIN      
        1 ccataccatc tttccgcctg agctcttgta agggttcccg ttatcgtcag gaaacggcgc
       61 aggaaacgat tctggaagcg gtccgtccgt catgagcgcg cgttccgtgg ggtagtcttt
      121 atacagattc gcaaacggcc gcagcgtccc aaaggggtcc agctcggcct gggccaaacc
      181 cgttttgctc atcgcgccgc tggagttctg gatcagttta ccgtttacgt tcgagaagct
      241 gtaccctttg aatgttgatg aagccacctg cgccggcgag agcggggcac cggttgtgtc
      301 atagatctgg ccgcgtgtat agaccgtgcc cgccacattg gagcaggctt cggttcggcg
      361 gtacaggagc gactccaacg atgcgacctt gacacgatca aaggtgccga atctcgccgg
      421 gtccgtgttc cgggcaaggt ccagcgcgtg aatctccgca tggcacagga tgcagttgat
      481 gttgttcgcc agtatcgcaa attcgaaccc ttcgaacagc ttgcctgaaa tgcggatggt
      541 ttgctggcag gtctgcgtcg cttgtcccag ttccgccgta gcgcgaagcg tcagggaggt
      601 acccgtcagg tcgtctgttc tggacaccgt cacttcagta atggtggcgc cattgcggag
      661 ccggcgggcc gaactcgagc caatgatggt cgcaggagca cccgtctcaa aaatcccgtt
      721 gccgttcagg tcgctttcgc ctgcatcacg cacaccgttt ccattgatgt cttcgttatt
      781 atcgacgagt tcatcgatat acagccggta cgacgccata tttcccgtcg tattgccatt
      841 tccgaccaca tagtcattcc agattctcgc tatcccgatg tcgacgccgg ccttgcatgc
      901 ctcatccaga aggaccttct gcacggcatc gtcagtgaaa cggccgcttg cgtgtacgcg
      961 catcaccgcc aatgcaaccg agccgagaac cagcatgatc gcaagtaacg cccacagcag
     1021 tgcaaagccg tcttgagaag tcttacggga aatcatggct tttctccctg gttgatcggt
     1081 caattgcaca catacacgcg cgccgatacg gtctccgata ccagggtggg tctccggccg
     1141 tccacgcgtg ctgttgcggt caaggtaatc gtcaggatgc tgttgtcgtc attcagagca
     1201 aactcaacgt cgcttaaatc gttacagcca ccgacggggc gcgcctcgcc gatgtctgct
     1261 gtatccccgt ccatgttgat atcctggatg cgcacgatgc ggcgcgtcag ggccccatcg
     1321 ccgtctgtat cttctccagc atccaacagg gcgtttcggt tgaggtcttc gttcatgtat
     1381 cgaaagcgga tgggcgagga ccatcgttga cgcgacccat ccagaggaac ctgcaagcgc
     1441 aattcgaccg ggcttccagg cacaggattt tggattacct caatttcttc aagaggcgga
     1501 atcagggaat tgtctccttg ccgggcggcg atttccgctt ctgcaatgat cgcggccatc
     1561 gtgtcgcgca cactggactg gacccggtct ttcgcgaggg caagggtcga ggaattcgcc
     1621 gcagtctcaa tggccacgaa gctcatgatc ccgatcactg caagcaggct catggcaatc
     1681 atcagctcga caagcgtgaa acccgattct ctcatcgagg cgctcctacc ggtcggtcag
     1741 gaccgtcgag acggtcatgg tcatggtgcg gttatgcaga tcgacccatg tgcttgtcag
     1801 tgtaatctcc agtgggttcg cagctgtatt gacgtaatcc acacggatgg tctcgttggt
     1861 ttgagttccc gccccgggga tgactgatcc gtcgggccat ctggtggtca cggcccctgg
     1921 aaaatcgtct ggatgctggt ctcgatgctg gcgcatctcg gtgaaaacac tcttggcgtt
     1981 ttccgcgacc gccaggcgtt ggctctgcat gagttgcgcc gcgtaaaacg aaatcaggat
     2041 ttgcgccaca cctcccagta ccaccatgaa gatggtgacc gcaatcataa tttcgagcag
     2101 cgtcatcccc gccttcgagg cggccacccc ctgcctcccc acgcgcatct gttcgttacc
     2161 gccagccatc tgccctgaag cctccatgaa tggacattcc cagtccaccg cccgtggaca
     2221 cttgcgagca cttgaattgt agcatatccc ataccactct cgagcttttc gtgcggtcga
     2281 ggcaaatttg agggcacagg cagatcgtta aattcacaaa gcattaattt acagagcatt
     2341 acgacttaga gcccagtttt gtcaggtgcg attctggcag ggcgggctgc atcctgtttc
     2401 taatgcggac tctgctgatt tccgccaaat tccgtcatat gggtacaatt tttgtcacaa
     2461 agaccgtctg cggtgcatcc tgctcccgtt ggatttagac aagtgcctgt ggggacatct
     2521 gcatcaattc ccaggccagg cgcgtcactt gaattacgat ctggcgtcgg gtagagtcag
     2581 ggctgcaggg gaaaagagca aatgcgggct ccgcaggaaa gcaggcagcc ctgaaacacc
     2641 ccgcggattc accaccacga taagctgcgg gaaggggcgg cgatcggcgc ctcaccgctc
     2701 tcgacgattg catttggcgg acgggctttt gttgcccgca aatggctcct gcccaggtgg
     2761 cagacagact acggtgcgaa tcaacgcggt tgctcaattt ccgctgaatt gaggtcgcag
     2821 ggtcgatgga atgtcggaat ggaggaatga cgcaaaatgg gtactaaagg acgtgagatc
     2881 gtgggtgtgg acgtgccgca gctcttggaa atgctcaaca aggctttcgc cgacgaatgg
     2941 cttgcttatt atcagtactg ggtcggcgcg agagtagcct ccggccggat gcggggcatt
     3001 atcgcccatg aactcgaaga gcatgccgga gaagag
//
LOCUS       DAOPBQ010000472         2997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26031_length_2997_cov_3.555065, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000472 DAOPBQ010000000
VERSION     DAOPBQ010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26031_length_2997_cov_3.555065"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="PKW60_13005"
     CDS             <1..1281
                     /locus_tag="PKW60_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00398.1"
                     /translation="GWLASRTATIALFWGLLALWSHMRWREEGRSEWLALSVSGLVLS
                     LLSKEEGISTCAYLAAYAVFLDPGRLTRRILSLLPYAGVALIWRLLYQGLGYGVTGSA
                     SCCDPVSAPGQFALLALCRAPVLFLGQWGLPPSDLHLALPEGGQWLLWFAGVLLFVVL
                     AVVFVPMARTDARIRFWSVGLGLSLLAACASLPSDRLLLWPGIGACGLLGQYLHARAE
                     RREIWSRAEAARILAPPLTFLLISVHVVLAPLLLPMRILAFATLGDIIEESVVAAPLP
                     SDVENRTVIFANAPNVYFTSYFPIIRHVLALPVPARVRTLSANAPLPQPHDIARINEK
                     TLRVTPRGGFAWYLMRDNAHPFSPGDTIDLGDIQVEITAVTRQGRPKEVEYHFETSVD
                     DPRYVWLVYKANQGQFAPFLPPPLGQVFTTDPYL"
     gene            complement(1306..2016)
                     /locus_tag="PKW60_13010"
     CDS             complement(1306..2016)
                     /locus_tag="PKW60_13010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPK00399.1"
                     /translation="MAVQAITFDFWCTLFRDAEKDERQQLRVDAVHEATGAPHEAIHA
                     ALETVWREFSRHHLEEQRTLRPEDAVHLAAQELGVTLESAVRRHLAEVFATAIVHHSP
                     VPIDGAFEAVRRAAKTMPIALVSDTGVSPGRSLKQLMDRHGFTPHFTALVFSDEVGVS
                     KPQPRMFEAAARALGVETGNLLHIGDLEPTDIAGAHAVGAKAALFTGDNSTYGESTAA
                     DYVFTHWNDFIARLAEIV"
     gene            complement(2186..2464)
                     /locus_tag="PKW60_13015"
     CDS             complement(2186..2464)
                     /locus_tag="PKW60_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00400.1"
                     /translation="MYRLFILALALTVTAAFMGCGGKAETPAPAPSSPAVTAPAQAPE
                     QSAPDAQVEAAPEAPAAQSGDAASASDEDDWGEFEVVPSASASAQKKE"
BASE COUNT          496 a          961 c          922 g          618 t
ORIGIN      
        1 ggctggctcg ccagccgcac cgcgaccatc gccctgttct ggggactgct cgcgctgtgg
       61 tcgcacatgc gctggcgcga agaaggccgc agtgaatggc ttgcactgag tgtatccggc
      121 ctggtgttga gcctgctctc gaaagaagag ggcatttcca cctgcgccta tctcgccgcc
      181 tacgccgtct ttctcgatcc cggccgcctg acgcgccgaa tcttgtcatt gcttccctat
      241 gccggagtgg cgctgatctg gcgtcttttg taccaggggc tcggatatgg ggtcacaggc
      301 tcggcctcct gctgcgatcc ggtatcagcg ccgggacagt tcgcgctgct ggcgttatgc
      361 cgcgctcccg tgctgtttct cggtcaatgg ggtctgccgc cctcagacct tcatctggcc
      421 cttcccgagg gcgggcagtg gctgctgtgg ttcgcgggtg tgctcctgtt cgtggtgctc
      481 gccgtggtct ttgtcccgat ggctcggacc gatgcccgaa tccggttctg gtccgtgggt
      541 ctggggcttt ctctgctggc agcctgcgcg tcgctgccgt ccgaccggct gttgctctgg
      601 ccgggcatcg gcgcttgcgg cctgctgggc cagtaccttc acgcgcgcgc cgagcggcgt
      661 gagatctgga gccgggccga ggccgcgcgc atccttgccc cgccgctcac cttcctgctc
      721 attagcgtcc acgtggtgct tgcgccactg ctgctgccca tgcgcattct cgcgtttgct
      781 accctgggcg acatcatcga agagagtgtg gtggccgcac cgcttccttc ggacgttgaa
      841 aacagaacgg tgatcttcgc caacgcgccc aatgtgtatt tcaccagtta ttttccgatc
      901 atccggcatg tcctggcgct tcccgtgccg gcacgcgtgc gcacgctatc ggccaacgcg
      961 cccttgcctc aaccccacga catcgctcga atcaatgaaa aaaccctgcg cgtgaccccc
     1021 cgcggcggat tcgcctggta cctgatgcgg gacaatgccc atccgttttc ccccggagac
     1081 acaatcgacc tgggcgatat ccaggtcgaa atcacggcgg taacccggca gggccgcccg
     1141 aaagaagtgg aataccattt tgagacgtcc gtggacgatc cccggtacgt ctggctcgtg
     1201 tacaaggcga accagggcca attcgcgccc ttcctgcccc cgcccctggg acaagtcttc
     1261 acgaccgacc cgtatctgtg aagcgcgaga tccgccgccc aggactcaga cgatctcggc
     1321 caaccgggcg atgaagtcgt tccagtgcgt gaacacgtaa tccgcggcgg tggattcgcc
     1381 gtacgtggag ttgtcgccgg taaacagggc ggctttggcg ccgacggcgt gcgcgccggc
     1441 gatatcggtg ggttccaggt cgccgatgtg gagcaaattc ccagtctcga cacccagggc
     1501 ccgcgccgcc gcctcgaaca tccggggctg cggctttgat acgcccactt cgtctgagaa
     1561 gaccagcgcg gtgaaatgag gtgtgaatcc gtggcgatcc atcagctgct tcagggaacg
     1621 gcccgggctg acgccggtat cggacaccag cgcgatgggc atggttttcg ccgcgcgccg
     1681 gaccgcttca aaggccccat cgatgggcac ggggctgtgg tgcacgatcg cagtcgcgaa
     1741 tacctcggcc agatggcgcc ggacggcgga ttccagtgta acgccgagct cttgcgcggc
     1801 caggtgtacg gcatcttccg gccggagggt gcgctgctct tcgaggtggt gccggctgaa
     1861 ttcccgccag acggtttcca gagcggcatg gatggcttca tgcggcgcac cggtggcctc
     1921 gtgtaccgcg tcaacgcgga gctgctggcg ctcgtctttt tccgcatcgc ggaaaagcgt
     1981 gcaccagaaa tcaaatgtga ttgcctgtac agccatactg gtcgcgtggt ggcctttcat
     2041 acgtaacatc ggtgtctggc gctatggcct gtaccggata ttacagcaat gcggcacgcc
     2101 tccagatttt ttcgaagcgc gccaaaaaac gaccggcctc tctcccgata ccggggaggg
     2161 aggccggaac aatctcaaac ctgcgctatt cttttttctg tgcgctggca ctggcggacg
     2221 ggacgacctc aaattctccc cagtcgtcct catcactggc gctggctgcg tcgccggact
     2281 gagccgcagg ggcttcgggg gccgcctcta cctgcgcgtc cggagcgctt tgctcaggcg
     2341 cctgcgccgg agccgtcacg gcggggctgc tcggcgccgg ggcaggtgtt tcggcctttc
     2401 cgccgcaacc catgaacgcc gcggtcaccg tcaaagccag agcgagtata aacaatctgt
     2461 acatcggtcg tctccttaca cctgtgttgg gcttgccaac ctggttaccc gaatggtttt
     2521 gcgccattcg gccaattttt cacgcgggaa gacaatccca gcgcattgat tccaatgtct
     2581 tgcctcgaaa gcggttgaag aaccggccgg gcgttaccat cgcgcgctga tggcgccctt
     2641 ttcgccggaa cttacacgca cgtatgatat aaccctcaaa ccaccgaacg caagccgggg
     2701 tcaacgcgtt cattcgcttg tttagacacc ggatccatcc gaaagttcat catttcgcga
     2761 aatgccggag gtgctgggca tcatcccggc ggatgcatgt cccagactgg catcaggtgc
     2821 gatggccgaa cttccgctgt ggtaaccggc caaatcgagg gtaacgaaac ggtaaccggc
     2881 gtcacgacag cttcttacga tggcgtcgcg gagcccgggc tcaaggagtc tggcgaattc
     2941 gacgggttcg acctcgatcc ggcacaaatc gccgtgatgc cggacgcggt actggtg
//
LOCUS       DAOPBQ010000473         2997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26033_length_2997_cov_3.219579, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000473 DAOPBQ010000000
VERSION     DAOPBQ010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26033_length_2997_cov_3.219579"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PKW60_13020"
     CDS             complement(<1..544)
                     /locus_tag="PKW60_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00401.1"
                     /translation="MAHPVRRGLSHRKSIVVAAILALVLAAPSPMTGYQLDDWLHHLK
                     LSARAADMGDGAPSSAMFCLADGEPQHTRPLVQSGFLPWWTLDTVRLSFWRPLAVLTH
                     VLDHSLWPDSYFMMHLHSLAWFGLAVLMAGMVYRRLLAPTWAAGLAALLFAIDDAHAM
                     PAGWLADRSAAIALFWGLLAL"
     gene            complement(879..2354)
                     /locus_tag="PKW60_13025"
     CDS             complement(879..2354)
                     /locus_tag="PKW60_13025"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HPK00402.1"
                     /translation="MRFSKRFAGSLVIVGFAVALGLTLLSVTAAQAQAPEERVFRAGA
                     AASNITPPLGVSLGGAMRDRSAAHVRDELHARCIVLDDGSVRIALVQVDNCLIPRQVF
                     DAAKKLIEDETGIPASHVLMAATHSHSTPTATGIFQSDPNVDYLPFLQRRIADGVRRA
                     ANNLEPARIAWGSGELPGEVFNRRWFLTPDTVLENPFGGRDQVRMNPGRASEALVEPA
                     GPTDPEISLIALESVDNRPIAVLANYSLHYVGGISDSHVSADYYGMFAERIQELLGAD
                     RLQPPFVGIMSNGTSGDVNNINFREPGKSQQPGEQMAHVAQAAADVVYAAYNRLEWHD
                     WVALGAVTKELELGVRLPSEEDVARAREIVAAAKGPIMETMPEIYARETILLNEYPEK
                     VIAPVQALRIGSLSIAAIPCEVFAEMGLQIKAESPFEDAFTIELANGYYGYLPTPRQH
                     ELGGYETWRARSSYLEPNASEVIMAAVKDLFQQLYPGEQSE"
     gene            2620..>2997
                     /locus_tag="PKW60_13030"
     CDS             2620..>2997
                     /locus_tag="PKW60_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK00403.1"
                     /translation="MRKKGFTLIELLVVIAIIGILAAILLPALARAREAARRASCANN
                     LKQWGLIYKMYSNESPGEAFPPVAPFANPNGVPIFASPDPDAIYPEYVTDLEVAHCPS
                     DTDADAPGAYVAGRLPDGTIEQHI"
BASE COUNT          601 a          926 c          896 g          574 t
ORIGIN      
        1 acagcgcgag cagtccccag aacagcgcga tggccgcgct gcggtctgcg agccagccgg
       61 cgggcatggc gtgtgcatcg tcgatcgcga acaatagcgc cgccaacccg gccgcccagg
      121 ttggcgccag aagcctgcga tagaccatac ccgccatcag caccgccagt ccgaaccagg
      181 ccaggctgtg caagtgcatc atgaaatacg aatccggcca caggctgtgg tcgagaacat
      241 gcgtcagcac cgcaagcggc cgccagaacg acagccgcac ggtgtccagc gtccaccacg
      301 gcagaaatcc cgactgcacg agcggccggg tgtgctgcgg ctcgccgtcg gccaggcaga
      361 acatggccga tgatggcgcg ccatcgccca tatccgccgc cctggctgat aatttgagat
      421 gatgcagcca gtcatcgagc tggtatccgg tcataggcga cggagccgcc agaaccagcg
      481 ccaaaatggc cgcaactaca atgcttttac gatgcgataa gcccctccgg acaggatgtg
      541 ccattgttcc ggctccccca tgaatgaaaa taaatccaat ctcatctaat tataatcaaa
      601 aatatgctga aatggggtgt gcgacaagaa attctttggt gatttgccga tcgtgcccaa
      661 agcgctcagc gggccctcca aaaacgcgta ttgaggcgct gcagcttgga atacacctgc
      721 atttcagctc agagaggggg taatgctggt gcaaatcgac ccccggccgg tttatttggc
      781 cggagggtcg aaattggcgt catgcagccc caaaaaccgt gggctcggag gtggcggatc
      841 gatctcctgc gctgggcgcg atgaagtacc gggtttcgtt attcgctctg ttctcccgga
      901 tacagttgct ggaacaggtc cttgaccgcg gccatgatga cctcggaggc gttgggttcc
      961 agatagcttg atcgggcgcg ccaggtctcg tagccgccga gttcgtgctg gcgtggcgtc
     1021 ggcaggtagc cgtagtatcc gttggcaagc tcgatcgtga atgcgtcttc aaaggggctt
     1081 tcagccttga tctggagccc catctcagcg aaaacctcgc aggggatggc agcgatgctg
     1141 aggctgccga tgcgcaacgc ctgtaccggc gcaatcactt tctcgggata ttcgttcagc
     1201 aggatggtct cccgcgcata gatttccggc atcgtttcca ttatgggtcc tttggcagca
     1261 gcaacgattt cgcgcgcgcg cgcgacgtct tcctcactgg gaagtcggac gccgagttcc
     1321 agctccttcg tcacggcgcc gagggcgacc caatcgtgcc attccagacg gttgtacgcg
     1381 gcatacacga catctgcggc cgcctgcgcc acgtgtgcca tctgttcgcc gggctgctgg
     1441 ctcttgcccg gttcccggaa attgatgttg ttgacgtcac cgctggtgcc gttggacata
     1501 atgcccacaa agggcggctg gagccggtcc gctcccagga gttcctgaat gcgctcggca
     1561 aacatgccgt agtagtccgc ggaaacatgg gaatcggata tgcccccgac ataatgcagg
     1621 gaataattgg ccagcactgc gatggggcgg ttatcgacgg attcaagcgc gatgagggag
     1681 atctccgggt cggtcgggcc ggccggctcg accagggctt cgctcgcgcg cccgggattc
     1741 atgcgaacct ggtcgcggcc gccgaaagga ttctcaagca cggtgtccgg cgtaaggaac
     1801 cagcgccggt tgaacacctc gccgggaagt tcaccgctgc cccaggcgat ccgcgcgggt
     1861 tccaggttat tggccgcgcg acggacgccg tcggcaatgc gccgctgcag aaacggcaga
     1921 tagtctacgt tgggatcgct ctggaagatg cctgtcgccg tgggcgtgga atgactgtgc
     1981 gtcgcggcca tcaatacgtg cgacgcgggg atgccagtct cgtcttcgat gagctttttg
     2041 gctgcgtcga acacctgccg cggaatcagg cagttgtcca cctgcaccag ggcgatgcgg
     2101 accgagccgt catccagtac tatgcagcgc gcgtgcaatt catcgcgcac gtgggctgcg
     2161 ctgcggtcgc gcatggcgcc gccgagcgat acgcccagcg gcggggtgat gttgctcgca
     2221 gcagcgcccg cgcgaaacac ccgctcctcc ggggcctgcg cctgggctgc cgttacgctc
     2281 agcagagtca aaccaagcgc cactgcaaaa ccaacaatta caagactgcc tgcaaaccgt
     2341 ttgctgaatc gcacgtcaga tacctcaccc gttttgaaaa tccagaagtt gctccggcaa
     2401 ccatgtcacc tgaaaacagt atacccaaga acgccgtcca agtcagatta accgtacaga
     2461 ccgccaatat actccccgca tttccagagc cgcgacgcgg ccaaaaggcc gcccgcaccg
     2521 ggccgttttc gttgtttgca tttgcctgat cgggagtgct aaagtattac gaatgtccaa
     2581 atcgtaacac tggcccacgc tgttttggag gaccgcttca tgcgcaaaaa gggattcaca
     2641 ctcattgaat tgctggttgt tattgccatc atcgggattc tcgccgccat cctgttgccg
     2701 gccctcgctc gtgcccggga agccgcccgg cgcgccagtt gcgccaacaa cctgaagcag
     2761 tgggggctga tctacaagat gtattccaac gagagcccgg gagaagcctt cccgcccgtg
     2821 gcgccgtttg ccaatcccaa cggcgtcccc atttttgcct cgcccgatcc ggatgccatt
     2881 taccccgagt atgtgaccga tctggaggtc gctcactgcc cgtcggatac ggatgcggac
     2941 gcgccgggcg cgtatgtggc aggccgcctg cccgacggca ccatcgaaca gcacatc
//
LOCUS       DAOPBQ010000474         2988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26132_length_2988_cov_3.838391, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000474 DAOPBQ010000000
VERSION     DAOPBQ010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26132_length_2988_cov_3.838391"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /gene="ilvB"
                     /locus_tag="PKW60_13035"
     CDS             <1..1518
                     /gene="ilvB"
                     /locus_tag="PKW60_13035"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPK00404.1"
                     /translation="DCMADSIPVVMISGQVPCPSIGTDAFQEAPVFNVMSACSKHVFL
                     CKDPDRLEDTVRTAFKLARSGRPGPVVIDVPKDVQNAIRPFRGEGYLSFRGYEERMTA
                     LGASRISKPEATAFFKMLEKSKRPLIYAGGGVIYAGAAQELRKFAKRFNIPVVTTLMG
                     IGSMDIADKLCLHMLGMHGTAYANYAVEDCDFVIAIGARFDDRVAGKIDDFAPNARYF
                     AHIDIDAAEIGKVRPVDWAHVGCARQALLDLLAYGKDFTKDFSKWVKHVGELKATHRL
                     NYDRDSDLIQPYEIIEELNELTGGEAIVCTGVGQHQMWAAQYCRMRHPRSFITSGSMG
                     TMGFGLPAAVGAQLACPDRTVIDIDGDGSIRMNFGELETLTTYNVPVKVLVLNNLGDG
                     MVRQWQSMYFGQRYSGSDKSLHRKDFVKAAEADGLTFAKRLDKRADIKRVLKQFVTHD
                     GPAFLEVMIDPYAKVFPMVGPGMSYKEMDTGDFIVSRSVQEKPEKSDMMKVPELF"
     gene            complement(1546..2391)
                     /locus_tag="PKW60_13040"
     CDS             complement(1546..2391)
                     /locus_tag="PKW60_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HPK00405.1"
                     /translation="MAHIGQADETSRKRLAALRHFLLDLDGTLYLGSNPIDGAAAFVR
                     YLVESGRKRLFFTNNCSVDAAYYEEKLARMGIPAARDDILTSGEATVRYLATGTPYRR
                     VFVLGTPSFEAELRAGGLTLVDDDAEAVVLAFDRTLTYAKLETACGLLRDGVPYFATN
                     PDKVCPTATGYIPDCGSMAALLFESTGRWPKYIGKPNPEMVAMGLQKLGGQAQDKAIT
                     AMVGDRLYTDIQMARQSGIAAILVLSGETRESDLANAEQTPDYVFASVKALHAALRDA
                     DRAMA"
     gene            complement(2397..>2988)
                     /locus_tag="PKW60_13045"
     CDS             complement(2397..>2988)
                     /locus_tag="PKW60_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPK00406.1"
                     /translation="EGAIGDLDADKRADAVVRHQKWIDVTEYLGGHTIRMNWVGAAND
                     VASRPAELKAFIDRCVPEFRKLCDYGDSKNINVVIENHGGASSEPGALIQLMVAVDHP
                     RFGTLPDFGNFPDHVDKYLATDLMMNFAKAVSAKCHDFDDETGLETTMDYERLIAIVV
                     DQHGYHGHIGIEFEGGRLSEFEGIKACKKLLETLQG"
BASE COUNT          589 a          918 c          901 g          580 t
ORIGIN      
        1 gactgcatgg ccgattcgat cccggtggtg atgatcagcg gccaggtgcc ctgtccttca
       61 atcggcacgg atgcgttcca ggaagcgccc gtgttcaacg ttatgagcgc atgctcaaaa
      121 cacgtgtttc tctgcaaaga ccccgaccgg ctcgaagaca ccgtgcgcac ggcattcaag
      181 ttggcgcgga gcggccgccc tggcccggtg gtcattgacg taccgaagga tgtgcagaac
      241 gccattcgcc cgttccgggg cgaggggtat ctgtcgttcc ggggctacga ggaacgcatg
      301 accgccctgg gcgcgtcgcg catttcgaag ccggaagcca ccgcgttctt caaaatgctt
      361 gaaaagagca aacgtcccct catctatgcc ggcggcggag tgatctatgc cggtgccgcc
      421 caggaactgc ggaaattcgc aaaacggttc aacatccccg tggtgaccac gctcatggga
      481 atcggcagca tggatatcgc ggacaagctc tgcctccaca tgctgggcat gcacggcacg
      541 gcctatgcca actacgcggt ggaggactgc gacttcgtga tcgcgattgg ggcgcgcttc
      601 gacgaccgcg tcgcgggaaa aatcgacgat ttcgccccga atgccaggta tttcgcgcat
      661 attgacattg acgcggcgga gattggaaaa gtccggccgg tcgactgggc gcatgtcggc
      721 tgtgcgcggc aggccctgct ggatctgctg gcctatggca aggatttcac gaaagacttt
      781 tccaaatggg tcaaacatgt cggagaactg aaggccaccc accggctcaa ctatgaccgc
      841 gacagcgacc tgattcagcc ttacgagatc atcgaggaac tcaatgaact caccggcgga
      901 gaggccatcg tatgcacggg tgtgggacag caccagatgt gggcggccca gtattgccgc
      961 atgcgccatc cgcgttcgtt catcacctcg ggcagcatgg gcaccatggg attcgggctg
     1021 ccggcggcgg tcggtgcgca attggcctgt cccgaccgca ccgtcatcga catcgacggc
     1081 gacggcagca tccgcatgaa tttcggggaa ctcgagaccc tcaccaccta caacgtaccg
     1141 gtcaaggtcc tggtgctgaa caatctcggc gacggcatgg tgcgccagtg gcagtcgatg
     1201 tatttcggac agcgttactc gggcagcgac aaatccctgc atcgcaagga ttttgtgaaa
     1261 gccgccgagg ccgacggcct tacctttgcc aaacggctcg acaaacgagc cgacatcaag
     1321 cgcgtgctga aacaattcgt cacgcacgac ggcccggcat ttctggaggt catgatcgac
     1381 ccctacgcca aggtgttccc gatggtcggc cccggcatga gctacaagga gatggacacc
     1441 ggcgatttca tcgtctcccg gtccgtgcag gaaaaacctg aaaaatcgga catgatgaaa
     1501 gtccccgagc tgttctaaac cgcatcagca cgctgacgct gagagtcagg ccatcgcgcg
     1561 gtccgcgtca cgcagcgccg catgcagggc cttcacggac gcaaacacgt agtcgggcgt
     1621 ctgctccgcg ttcgcgagat ccgattcgcg cgtctcgccg ctcaacacga ggatcgcggc
     1681 aatgccggac tgacgggcca tctggatgtc ggtgtagagc cggtcgccta ccatggcggt
     1741 tatggcttta tcctgcgcct ggcccccgag cttctgcagg cccatggcca ccatctcggg
     1801 attgggtttg ccgatgtatt tgggccaccg gcccgtgctc tcgaacagca aggcggccat
     1861 cgaaccgcag tcggggatgt atccggttgc ggtgggacaa accttgtccg gattcgtcgc
     1921 gaagtacggg acgccatcgc ggagcagtcc gcacgctgtc tccagtttcg cataggtcag
     1981 cgtccggtca aacgccagca caaccgcctc cgcatcgtcg tcgaccaggg tgaggccgcc
     2041 cgcgcggagc tctgcttcaa atgagggcgt ccccagcaca aacacgcggc ggtatggagt
     2101 cccggtcgcg agatagcgca cggtcgcctc gcctgatgtg aggatgtcgt cgcgcgccgc
     2161 cggtatgccc atcctcgcga gcttttcttc gtagtacgcg gcgtccacgg aacagttatt
     2221 ggtgaaaaag aggcgtttac ggcccgattc cacgaggtag cgcacaaacg cggccgcgcc
     2281 atcgatgggg ttcgatccca gataaagggt gccgtccaga tcgagcagaa agtgccgcaa
     2341 cgcggccagc cgtttcctgg acgtctcatc cgcctgcccg atatgcgcca tccggcttat
     2401 ccctgaagcg tttccaggag ttttttgcag gccttgatgc cctcgaactc gctgaggcgg
     2461 cccccttcga actcgatgcc gatgtggccg tggtatccgt gctggtccac cacgatcgca
     2521 atgagccgtt cgtaatccat ggtggtctcc aggccggttt cgtcgtcaaa atcgtgacac
     2581 ttggcgctga cggccttcgc gaagttcatc attaaatccg tggcgagata cttgtccacg
     2641 tggtcgggga aattgccgaa atcaggcaac gtgccgaaac gcggatgatc cacggccacc
     2701 atgagctgaa tgagcgcccc gggttctgac gaggctccgc cgtggttctc gatcacgacg
     2761 ttgatgtttt tcgagtcgcc gtagtcgcac agctttcgaa attccggcac gcagcgatcg
     2821 atgaaggcct tcagctcggc ggggcgcgat gcgacatcat ttgctgcgcc gacccagttc
     2881 atgcggatcg tgtgtcctcc caggtattcg gtaacatcga tccatttctg gtggcgaacc
     2941 acggcgtccg cccgtttgtc cgcatccaga tcgccgatcg caccctcg
//
LOCUS       DAOPBQ010000475         2988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26133_length_2988_cov_3.719059, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000475 DAOPBQ010000000
VERSION     DAOPBQ010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26133_length_2988_cov_3.719059"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKW60_13050"
     CDS             <1..289
                     /locus_tag="PKW60_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00407.1"
                     /translation="LHAMDAAQISERTFCGMAMKTAAVDWSRCRLCKNGALANRAHPS
                     GNPDRTAALCIRTCVHHIEEAGLVANTFQRPFRQRPAWRLDLNGTARAGEI"
     gene            293..1354
                     /locus_tag="PKW60_13055"
     CDS             293..1354
                     /locus_tag="PKW60_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00408.1"
                     /translation="MAAITSSQVKEFALSQDLDLAGVANIERFDGAPARMHPAAIMPE
                     VKSVIVVARRILRGNWRGIEEGTYWPNYTYFGYHGLLNSFFIPRGVYETACFIEDCGW
                     EAVPYYPGVPEAQPPVEPVRPGAVGPDVHLAIRIAGIAAGLGELGWSKVFLTRRFGPR
                     VRLAAILTDAVLEPDPLLEPGSLCDQCMACVQGCPDAIPHKQEEKTVSICIEGKTYEW
                     ADVHMGRCTLCYHGGDPRVSPFIHKTFPGYEFDVRRQDISEEAAYKLCWPFSLGKWRP
                     TGEDPSGYLIEGHVRLQEWGGDGSYGIEGSRGCMRSCFDHLEQSGRVEQTFANGPFIK
                     RPRWLLSHRVPHPGAGDTA"
     gene            complement(1450..2349)
                     /locus_tag="PKW60_13060"
     CDS             complement(1450..2349)
                     /locus_tag="PKW60_13060"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPK00409.1"
                     /translation="MRKTACFASRNALARPAQHTAITLAAGLLVCILLRLATASAASA
                     GAPSLDWTPVFEGIQYRQLIQGEDEPLRAHALRVDLKHPDIRFFVTPANGDRAKDTDG
                     MKTTTFLKKYGCQVAINASPYAPVGEVEGEPCDVLGLSISEGDAYSAAHGAWGALLIT
                     KDNRAQIATPPFDISDVYNAVGGFHLLLKDGANVGTDGEKHPRTAVGISRNGRYLYLL
                     VIDGRQPGYSLGASTRETADLMKTLGACDALNLDGGGSATLVFDGGQGKPKVINRPIH
                     LLPGMERVCANHLGIFAKPLEGK"
     gene            complement(2567..>2988)
                     /locus_tag="PKW60_13065"
     CDS             complement(2567..>2988)
                     /locus_tag="PKW60_13065"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPK00410.1"
                     /translation="GVPGWLESCVAEQKETSGMFELVALMLLSQQPPEPFVEGMASYY
                     TVASSSGVTASGERMRDTELTCAMREGEFGHYYLVVAANGRSVVVRLNDRGPYARRRV
                     IDLSKAAMRELHPSQGLMWVKVYDLGGSPPSELAAKQ"
BASE COUNT          584 a          975 c          864 g          565 t
ORIGIN      
        1 tctgcacgcc atggacgccg cgcagatcag cgagcgaacc ttctgcggga tggcgatgaa
       61 gacagcggcg gtggattgga gccgctgccg cctctgtaaa aacggcgccc ttgccaaccg
      121 cgcccatccc agcggcaacc cggatcggac tgccgccctc tgcatccgca cctgcgtgca
      181 tcacattgaa gaagccggcc tggttgcgaa cacctttcag cggccgttcc gccaacggcc
      241 ggcctggcgc ctcgatctga acggcaccgc ccgtgccggg gagatttgag tcatggcggc
      301 catcacctca tcgcaggtta aggaatttgc gctcagtcaa gacctggatc tggccggcgt
      361 ggccaatatt gagcgcttcg acggcgcgcc cgcgcgcatg catcctgccg ccatcatgcc
      421 cgaagtaaaa agcgtaatcg tcgtcgcccg ccgcatcctt cgcggcaact ggcgcggcat
      481 cgaggaaggc acgtactggc ccaattacac gtatttcggg tatcacgggc tgctcaacag
      541 ctttttcatc ccccgcggcg tctatgagac ggcctgcttt attgaagatt gcggatggga
      601 agctgtgccc tactacccgg gcgtccccga ggcccagcct cccgtggaac ccgtccgccc
      661 gggcgccgtc gggcccgatg tgcacctcgc cattcgcatt gccggcatcg ccgcgggact
      721 cggcgagctc ggctggtcga aggtcttcct cacccgccgg tttgggccac gcgtccggct
      781 cgcagccatc ctcaccgatg cagtgctcga accagacccc ctcctcgagc cgggctcgct
      841 ctgtgaccaa tgcatggcct gtgtgcaggg ctgtcccgac gcgatcccac acaaacagga
      901 ggaaaagacc gtttccatct gcatcgaggg caagacctac gaatgggccg acgtccacat
      961 gggccgctgc acgctttgtt atcacggcgg cgacccgcgc gtatccccct tcattcacaa
     1021 gacatttccc gggtatgaat ttgacgtacg ccgccaggac atctcggaag aagccgccta
     1081 caagctgtgc tggccgttct ctctcggcaa atggaggccc accggcgaag atccgtccgg
     1141 ataccttatt gaagggcacg tccgcctgca ggaatggggc ggcgacggaa gttacggcat
     1201 tgaagggtcg cgcggctgta tgcggagctg tttcgaccat ctcgaacaaa gcggccgagt
     1261 tgaacagacc ttcgcgaacg gcccgttcat caaacgcccc cgatggctgc tctctcatcg
     1321 cgtcccccat ccaggagcgg gcgacacggc ctgagtgccg gtttctgtgt tctgagggcc
     1381 ctgtgagccg gcccgccagc cgtttttctg ggggcggcga tatggcgcgg acaaaacggg
     1441 aaaaacaggc tacttgcctt caagcggctt ggcaaagatc cccaaatggt tcgcacacac
     1501 ccgctccatg cccggcagca ggtggatcgg gcggttgatc actttcggct tcccctggcc
     1561 gccatcaaac accagggttg ccgagccgcc gccatccagg ttgagcgcat cgcaggcgcc
     1621 aagcgttttc atcagatccg cagtctcccg ggtagatgcg cccaggctgt aacccggctg
     1681 gcgcccatca atgactagaa gatagaggta gcggccattc cgcgagatgc ccacggccgt
     1741 ccgggggtgc ttctctccgt ctgtgccaac attggcccca tctttcagaa gaaggtgaaa
     1801 acctcccacc gcgttgtaga catctgaaat gtcgaatggc ggcgtcgcaa tttgcgcccg
     1861 gttatccttt gtaataagga gagcgcccca ggccccgtgg gcggcggaat acgcatcgcc
     1921 ttccgagatc gaaagcccga gaacatcgca gggctccccc tcgacctccc ctaccggcgc
     1981 atagggcgat gcattgatgg ccacctggca gccatatttt ttcaaaaacg tcgtcgtttt
     2041 catcccgtcg gtatctttgg cccggtcacc gttcgcgggc gtgacaaaga accggatatc
     2101 cgggtgtttc aaatcgaccc gaagtgcatg cgctctgaga ggctcatctt ccccctggat
     2161 cagctgcctg tattggatcc cctcaaaaac aggagtccaa tcgaggctgg gagcgcccgc
     2221 cgacgctgca gacgccgtgg caagccggag gaggatgcag accagaaggc ctgcagcaag
     2281 cgtgatggcg gtgtgctgcg caggccgcgc aagggcgttt ctcgaagcaa aacaggctgt
     2341 ttttctcacg agagcctttc agaattcttg acgcgcgggt gctttccacc gccaacaaat
     2401 gaacgggcgg ccgggctgcg cactccaatc tctttacctg tatataactg gatcctcgtc
     2461 gagaatccag ggtttctcca ggcaaatgag atcgcggctc tggaacggca gcacacagga
     2521 cggccggcga cgccacaggg caccgccggc cgttcctttg tgtaacctat tgttttgcgg
     2581 caagttctga aggagggctc ccccccagat cgtacacttt cacccacatc aggccttgtg
     2641 agggatgaag ctcccgcatc gccgccttgg aaaggtcgat cacccggcgc cgcgcatacg
     2701 ggcctcgatc gttcaacctc accacgacgg agcggccatt ggccgcaacc accagatagt
     2761 aatgcccaaa ctcaccttca cgcatggcac aggtcaactc ggtatcccgc attcgttcgc
     2821 cggacgcggt gacgccactg ctgctggcca cggtgtagta ggaggccatg ccttctacga
     2881 aaggttctgg cggttgttgc gacagtaaca tcagcgcaac aagctcaaac attccggagg
     2941 tctccttttg ctcggcgacg caagactcca gccagcccgg gactccca
//
LOCUS       DAOPBQ010000476         2987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26147_length_2987_cov_4.012960, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000476 DAOPBQ010000000
VERSION     DAOPBQ010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26147_length_2987_cov_4.012960"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKW60_13070"
     CDS             <1..462
                     /locus_tag="PKW60_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK00411.1"
                     /translation="DTLLMRMKDDDWAQVIDTDLTGVFRCCRAVVRGMMKQRWGRIIN
                     ISSVIGILGQPGQANYAAAKAGLLGLTRSLARELGSRNITVNAVAPGLIETDMTAAIQ
                     GDMREAMLARIPLGRAGSPEEVAGLVSYLASEAAGYITGATIAIDGGLTMH"
     gene            700..1044
                     /gene="ytxJ"
                     /locus_tag="PKW60_13075"
     CDS             700..1044
                     /gene="ytxJ"
                     /locus_tag="PKW60_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010899157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HPK00412.1"
                     /translation="MKEMTKQDDWQACLDASQVKPVFVFKHSTSCPVSGAAHSRVVEY
                     ETQNSGNGVDIHMVKVIESRPVSNAIAEALGVRHQSPQIILVKDRAAVWSASHLEVRG
                     DRMQEALEKACS"
     gene            1112..1435
                     /locus_tag="PKW60_13080"
     CDS             1112..1435
                     /locus_tag="PKW60_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00413.1"
                     /translation="MATVSGVHAGVAVEEFVSHAISIPRALDAIEAGALLASGRRVVL
                     KPNLVNHSPHPITTPPAARNYSGSIGGASATSAWPTVYWARLNPVPEARFGCVPDRPT
                     GCRRW"
     gene            complement(1413..2789)
                     /locus_tag="PKW60_13085"
     CDS             complement(1413..2789)
                     /locus_tag="PKW60_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPK00414.1"
                     /translation="MDLEKHAEGILATALRNGGQFAEIFLEETARTVIVFEDNKVEQI
                     VSGVDAGAGIRHLSGDQTLYGHTNELTEPHLTALANELAAGACGGKPDCNFAFTPERL
                     GKVPKVPPSAVPTPRKLELVRNANAAARAHDPRIVQVTVSYGDSLRRALIVNSEGRFV
                     EESRPQVLFSVRVVARDGAVVQTAYETAGGTLGFELFDEVDPEALALQAARRACLMLK
                     AEPAPTGRMPVVLSSEAGGTMIHEAVGHGLEADHIEKGISKYCGRLNEQIAVPEITVV
                     DDGTLPGKRGSACMDDEGTPTRRTVLIDGGVLVQFLNDRRTAWKMGMAPTGNGRRESY
                     QHKPVPRMTNTLILPGTSDPASILAETGTGLFVKKMGGGQVTPLNGDYVFEVSEGYLI
                     RNGEAAIPVRGATLIGNGPDTLMQIEAVGSDLGFAIGTCGKDGQAAPVSDAQPTLRIR
                     ELTIGGTR"
BASE COUNT          525 a          960 c          956 g          546 t
ORIGIN      
        1 gataccctcc tcatgcgcat gaaagacgac gactgggcgc aggtcatcga caccgacctt
       61 accggcgtgt tccgttgttg ccgggccgtg gtgcgcggca tgatgaaaca gcggtggggg
      121 cgcatcatca acatctcgag cgtgatcggc atcctcggcc agccgggcca ggccaattat
      181 gccgcggcaa aggccgggct gctcgggctg acccggtcgc tggcccgcga actcggttcg
      241 cgcaacatca ccgtcaacgc ggtagcgccg ggtctcattg agaccgacat gaccgcggcg
      301 attcagggcg acatgcgcga ggccatgctg gcccgtatcc cactcggccg cgccggcagc
      361 cctgaggaag ttgccgggct cgtgagctat ctcgcgtccg aggccgcggg ctacatcacc
      421 ggagccacga tcgccatcga cggcggcctc acgatgcact aaagcccacg cggggctgcc
      481 tgcgcgcgtt ttcagccggt gctccagagc gccatggccg caatagattc aatgcaggct
      541 gcgcagggac cgtggtgatt cctggccctg cctggaatcc tcgcagcggc tcgggtgttg
      601 tttgaaagaa cggggaccgg ttccaaacgt catccgcggc ggcaccgtgc gttcccgttt
      661 gaaggcgcac ctcttcaagg tcgttacagg agcggtacga tgaaagaaat gacaaaacag
      721 gacgactggc aggcctgcct ggatgcgtcg caggtgaaac cggtgtttgt gttcaagcac
      781 agcacgtcct gcccggtctc tggtgcggca cacagccgcg ttgtggaata cgaaacccag
      841 aattccggca acggggtgga catccatatg gtgaaggtga ttgagagccg ccctgtatcg
      901 aacgcgatcg ccgaggccct gggcgtgcgg caccagtcgc cgcagatcat cctcgtgaaa
      961 gatcgggcgg cggtatggtc ggcatcccac ctcgaagtgc gcggtgatcg catgcaggaa
     1021 gcgttggaaa aggcgtgttc gtagccgcga tatccggcct gagcagccgg gcggtctcgg
     1081 gcgttcacag gttggaagcg tgacgaaaag cgtggccaca gtatccggcg tgcatgcggg
     1141 cgttgcggta gaagaattcg tctcgcatgc gatctccata ccgcgtgcgc tcgacgccat
     1201 tgaggcgggc gcgttgctgg catccgggcg gcgcgtcgtg ctgaaaccca acctcgtcaa
     1261 ccacagcccg catcccatca caaccccgcc cgcggcgcgg aattactcgg gctcgattgg
     1321 cggcgcatcg gccacatccg catggccaac ggtgtattgg gccaggctga atccggttcc
     1381 tgaggcccgg ttcggctgcg tgcctgatcg gcctaccggg tgccgccgat ggtgagctcc
     1441 cggatgcgca aggtgggctg ggcatcgctg accggcgctg cctggccgtc tttcccacag
     1501 gtcccgatgg caaatcccag atcgcttccg accgcctcga tctgcatgag ggtgtcgggg
     1561 ccgtttccga tgagcgtggc cccgcgcacg ggaatcgcgg cctcgccatt gcggatgaga
     1621 tacccttcgc tcacttcaaa cacgtaatcg ccgttcagag gcgtcacctg ccccccgccc
     1681 atctttttga cgaagagccc cgtgcctgtc tcggcgagaa tggatgcggg gtcgctcgtg
     1741 ccgggaagaa tgagggtatt ggtcatccgt gggacgggct tatgctgata cgattcccgc
     1801 cgcccattgc ccgttggcgc catgcccatc ttccaggcgg tccggcgatc attcaaaaac
     1861 tggacgagta cgccgccatc gatcagaacc gtccggcggg tgggcgtgcc ttcgtcgtcc
     1921 atgcaggcgg aaccgcgctt accgggaagc gtgccgtcgt cgacgacggt gatctcgggc
     1981 acggcgattt gttcattcag ccgcccgcag tatttcgata tgcccttctc gatgtggtcg
     2041 gcttcgaggc cgtggccgac cgcttcgtgg atcatggtgc cgcccgcctc gctgctcagc
     2101 acgacgggca tgcgcccggt cggcgcaggt tcggccttga gcatgaggca ggcccggcgg
     2161 gccgcctgca gcgccagcgc ttcggggtcc acctcatcaa acaattcaaa cccgagggtc
     2221 ccgccggcgg tctcgtatgc ggtctgcacc acggcgccgt cgcgggccac cacccgcacg
     2281 gagaagagca cctggggccg gctctcctcc acaaagcgcc cctcggaatt gacgatcagc
     2341 gcgcggcgca gcgaatcgcc gtacgagacc gtcacctgca caatgcgcgg gtcatgggcg
     2401 cgcgccgcgg cgttggcgtt gcggaccagt tcgagcttcc gcggcgtggg cactgccgat
     2461 ggcgggactt tggggacttt ccccaggcgc tcgggcgtaa acgcgaaatt gcaatccggt
     2521 ttgccgccac acgcgccagc ggcaagctcg ttcgcaagtg cggtgagatg cggctcggtg
     2581 agttcattgg tatggccata gagggtctga tcgccgctga gatggcggat gcctgcgccc
     2641 gcgtcgacgc ccgaaacgat ctgttcgacc ttgttatctt caaaaacaat gacagttctg
     2701 gcggtttctt ccagaaaaat ctcggcgaac tgcccgccat tccgcaaggc cgtagccagg
     2761 atcccttccg catgtttttc cagatcaatc acgctgcacc ctttccggcg cgggaatctg
     2821 cccacgcgca cataccgtcc gcagtatcga taatgtggaa cgcgctccgc aacttcgggc
     2881 gcagctcagg cgctctgtca ctcctgttcg ggttcggacg atgctcgcgt gcgccttgac
     2941 caaaaggata gcactgcacc cgccagaagc aagacccctg cgaacac
//
LOCUS       DAOPBQ010000477         2983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26189_length_2983_cov_3.579235, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000477 DAOPBQ010000000
VERSION     DAOPBQ010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26189_length_2983_cov_3.579235"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKW60_13090"
     CDS             complement(<1..323)
                     /locus_tag="PKW60_13090"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPK00415.1"
                     /translation="MPADSTRNGTEARQTCAAFNLPNQLTLLRLLMTPVLLALLSVDS
                     LPTYAVGYALFIVASLTDYYDGKIARERNLITNFGKLMDPLADKILISTVFIMMVGMP
                     DLIVPR"
     gene            complement(453..950)
                     /locus_tag="PKW60_13095"
     CDS             complement(453..950)
                     /locus_tag="PKW60_13095"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPK00416.1"
                     /translation="MSTKTTFPGHELREHREARRLSLAEVYKRVHIPIRYLEALEQGR
                     FEDLPEPCFVSGFLKSYCAFLELNAERFINLYHEAASPTPARLLRHTRKAPEAVPEWR
                     RELMTWAAICAMLAAGWLAYTVVVRPDSGAGQGRVEAGAPDISERLTPPGNPFEHASG
                     SAGRR"
     gene            complement(947..>2983)
                     /locus_tag="PKW60_13100"
     CDS             complement(947..>2983)
                     /locus_tag="PKW60_13100"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPK00417.1"
                     /translation="ATVVALMVSAIGLLLSTDFLFVNALNALRMTATLILKIVAHWVE
                     VGADAWRAGSAARKSPAASLRKKDKAWARLRSSQSSAEETDEPELAPEPVERRIRIRS
                     GLRQSTQDEEPALETPELPFDDIPVEAPAAAFADPMEEPEAAEEALGDVEPEIAAPVE
                     ERVPARRKPLLPQRRVQPDEELPPDYEYPKRYTKPSLDMFDPPPPPAETDMEDHLYAT
                     SATLEDKLKTFGIEAQVVEVTRGPTITRFELEPAPGIKVSRFHALADDIALALRAYRV
                     RVEAPIPGKGRVGIEVPNKDRETVVVRELLESRAFSRSKYALPLALGKGIGGEAHVVD
                     LTAMPHLLVAGATGAGKTICVKTLLASLLVEKTPDDMQLMLIDPKMVELSTFNDIPHL
                     ITPVITEAKKAASGLNWLINEMEERYRLFAHLRTRNIEVYNASVENGEIEMPQKDGEA
                     SESMNVIRKLPYIVCVIDELADLMMLARAEVEDAIARLAQLARAVGIHLIIATQRPSV
                     DVLTGVIKANFPARISFQVASRVDSRCILDEIGAERLIGKGDMLYLPPGRSKPTRIQS
                     GFVSDEEMNRLVAYLKTQAPAQYREEIEKFGKTDDKLDDLADQDDDLFDDAVRVVLET
                     GQASISMVQRRLRVGYTRAARLIDMMELRGIVGPHIGSKAREILVNAPPKNEVA"
BASE COUNT          517 a          884 c          957 g          625 t
ORIGIN      
        1 cgcggcacga tcaggtctgg catgccgacc atcatgatga acaccgtcga tatcaggatc
       61 ttgtccgcca gcgggtccat gagctttcca aaattcgtga tcagatttcg ttcgcgggcg
      121 attttaccgt cgtagtagtc ggtcagcgaa gcaacgataa acagggcata gccgacggcg
      181 taggtgggaa gcgagtctac agagagcagg gcgaggagca cgggggtcat gagcagccgc
      241 agcagcgtga gctgattcgg caggttgaag gccgcgcagg tctggcgcgc ttcggtcccg
      301 tttctggtac tgtcagctgg cattggcata ttcatcacgg ctgcggacgt atttgatttt
      361 ttcgtgcggt ccaggatgcg gcgtcattgt agcagattgt tcagagcgag gaaatgtgat
      421 gccgggacgc cgcctttccg cgaatttgga aactaacgcc ggccggcgga tccgctggcg
      481 tgttcaaaag gattgcccgg gggagtaagg cgctcgctga tgtccggtgc accggcctcg
      541 acccgcccct gtccggcgcc gctgtccggg cgcacgacca cggtataggc cagccagccc
      601 gcagccagca tggcgcagat cgcggcccag gtcatgagtt cgcggcgcca ctcgggaacg
      661 gcttccggtg cttttcgtgt atggcgcagg agccgcgcgg gagtgggaga cgccgcctca
      721 tggtacagat tgataaaacg ttcggcattc agctcgagga aggcacagta ggatttgaga
      781 aagccgctga cgaaacacgg ttcgggcaag tcctcgaagc ggccctgttc gagcgcttcc
      841 aggtatcgga tcgggatatg cacccgtttg tagacttcgg ccaggctcag acggcgcgct
      901 tcccgatgtt cgcgcaattc atgtcccgga aatgtcgtct tggtgctcat gcgacctcat
      961 ttttgggtgg cgcgttgacg agaatctcgc gcgctttgct tccgatatgc ggacccacga
     1021 tccccctcaa ttccatcatg tcgatgagac gagcggctcg agtatacccc acccgcaacc
     1081 gtctttgcac catagaaatt gatgcctgcc cggtttccag aacgacccgg actgcatcgt
     1141 caaagaggtc gtcgtcctgg tcggcgagat cgtcgagttt atcgtccgtc ttaccgaatt
     1201 tctcgatttc ttcccggtac tgggcgggcg cctgggtttt caggtacgcg accagccggt
     1261 tcatttcctc atcgctgacg aatccgctct ggatacgcgt cggcttggaa cgtccgggcg
     1321 gcaggtagag catatcgccc ttgccgataa ggcgttccgc gccgatctcg tcgagaatgc
     1381 agcgcgaatc cacccgcgat gccacctgga acgagatgcg tgccgggaag ttggctttga
     1441 tgacgccggt gagcacgtcg acggagggcc gctgcgtggc gataatcagg tggatgccca
     1501 cggcgcgcgc cagctgggcg agccgggcga tcgcatcctc aacctccgcc cgggccagca
     1561 tcatcaggtc agccagctcg tcaatcacgc agacgatgta gggcagtttg cggatgacgt
     1621 tcatggattc gctggcttcg ccgtctttct gcggcatttc gatttcgccg ttttccacgc
     1681 tcgcgttgta gacctcgatg ttacgcgtgc gcagatgcgc gaagaggcgg tagcgctctt
     1741 ccatttcgtt gatgagccaa ttgaggccgg aggcggcctt cttcgcctcg gttatcacgg
     1801 gcgtgatcag gtgggggata tcgttgaacg tggacagctc gaccattttg gggtcgatca
     1861 gcatgagttg catgtcgtca ggcgtctttt ccaccaggag gctggccagc aacgtcttga
     1921 cgcagatggt ctttccggcg ccggtcgccc cggcaacgag gagatgcggc atggcggtca
     1981 ggtcgaccac atgggcctcg ccgccgatcc ctttgcccag agccagcggc agcgcgtact
     2041 tggagcggga aaacgcccgc gactcaagca attcacgcac gaccacggtt tctcggtctt
     2101 tgttgggcac ctcgataccg acccggcctt tcccgggaat cggggcttcg acccgcacgc
     2161 ggtacgcgcg cagcgccagc gcaatgtcat ccgcgagcgc atgaaaacgg ctgaccttga
     2221 tgccgggggc gggttccagt tcaaagcgcg tgatggtggg gccgcgcgtc acttccacca
     2281 cctgggcctc gatgccgaag gttttcagct tgtcctcgag tgtcgcgctt gtcgcgtaga
     2341 ggtggtcttc catgtcggtc tctgcgggag gcggcggcgg atcgaacatg tccagcgacg
     2401 gtttggtgta ccgtttgggg tattcatagt cgggaggcag ctcttcatcc ggctgcaccc
     2461 tgcgctgggg cagcagcggc ttgcgtcgcg ccggcactcg ttcctctacc ggcgcggcga
     2521 tctcgggttc cacatcgccg agtgcctcct cggccgcttc cggctcctcc atcgggtctg
     2581 caaaggctgc ggctggggcc tccacgggaa tatcatcaaa gggcaactcg ggggtttcga
     2641 gcgcgggctc ctcatcctgc gtgctctgac gcagtcccga gcggatgcga atgcggcgtt
     2701 cgacgggttc gggcgcaagc tcgggctcat cagtttcttc cgcggagctt tgggatgagc
     2761 gcaatctggc ccaggccttg tccttttttc gcaggctcgc agcggggctt ttccgtgcgg
     2821 ctgaccccgc gcgccaggcg tctgcgccaa cctcgaccca gtgcgcgacg atcttcagta
     2881 tgagcgtagc ggtcatccgg agcgcattga gggcattgac gaaaaggaaa tccgtcgaga
     2941 gcagaaggcc gatcgccgat accatcagcg cgaccactgt cgc
//
LOCUS       DAOPBQ010000478         2981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26207_length_2981_cov_4.096719, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000478 DAOPBQ010000000
VERSION     DAOPBQ010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26207_length_2981_cov_4.096719"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PKW60_13105"
     CDS             <1..369
                     /locus_tag="PKW60_13105"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPK00418.1"
                     /translation="ASSQPVLVPVTLGKGRMLWSRLESMEELAPAAFGVLEPRPEYRR
                     DMAPPADSVVLVPGLAFTESGWRIGYGGGYFDRFLAEFPGTKIAIAYALQLIPEMPAE
                     AHDIPMDIIVTESRVYRRPR"
     gene            complement(392..1309)
                     /gene="miaA"
                     /locus_tag="PKW60_13110"
     CDS             complement(392..1309)
                     /gene="miaA"
                     /locus_tag="PKW60_13110"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPK00419.1"
                     /translation="MKNLVAVVGPTASGKTALALELARRLDSEIISADSMQVYRGMEI
                     GTAAPTAQEQARAKHHFVSLLEPGEEFSAGLFGRRARQLVDALNARGRIAVVAGGAGL
                     YVRALIEGLFPGPGKDDSIRERLCREAEEAGVPALYARLQACDPAYAQVIHSNDLRRI
                     VRALEVHELTGDPLSQLHAGHKMAAVPLDAVQVALDWPREQLYERIDARVDRMIEQGF
                     VEEVQRLLDAGHAKHLYRLRSLGYREIAAFLAGEKTLDEAVASMKQNTRRFAKRQLTW
                     FRADPAIQWLSASPERSIESYADEILTLL"
     gene            complement(1306..>2981)
                     /gene="mutL"
                     /locus_tag="PKW60_13115"
     CDS             complement(1306..>2981)
                     /gene="mutL"
                     /locus_tag="PKW60_13115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPK00420.1"
                     /translation="GHGMSEQDALLAIERHATSKIRKADDIDNVVTMGFRGEALPSIA
                     SVSRFELVTRRASDEHATRIRIEGGVLRDVSQISAPPGTRVTVNRLYFNTPVRAKFLK
                     GITTELSQSIDIVQRHALASPGVAFQLLHNEKLLFDIPQNTSLKERIALIWGLGFAEE
                     LVALDGAGAGLSFSGFIGTPALTRSGRTHQFFFFNGRPVVNRSLQYGFEEGYRGLLTV
                     GRRPVGIVLVTAPPRLVDVNIHPAKREIRFRDENAARQALREATRNRLQRLTRTPQPA
                     AEPVYDDAASGQERARYSEGQRNTSISRPEPQTTIFDPRGTPAPSTPVRQPALDPPPP
                     AQTEFEGLPERKPREEAAGVEPRAFYASLGQISEAPIQLFATYLLVPEEDRLLIIDQH
                     ALHERLTFDTLLADLQDADYQAQQLVVPILVDVPPSYVKLLEEHHALFSKLGIEIEPF
                     GGSTFQVTALCHLYEDAAIPGAVHRVLDTLAQGDLFNREELVSDLLRLTVEACRSSVK
                     AGDRLSPEERRELLEGFRRLRPPYTCPHGRPIITELTQVQMEKSFRRRQ"
BASE COUNT          525 a          900 c         1005 g          551 t
ORIGIN      
        1 gccagttcgc agccggtgct ggtgcccgta actctgggca aagggcgcat gctgtggtca
       61 cgcctggaat cgatggaaga actggcgcct gcggcctttg gcgtgctcga accccggcct
      121 gaataccgcc gggatatggc gccgccggcg gacagcgtgg tgctggtgcc ggggttggcg
      181 ttcacggaat cagggtggcg catcggttac ggcggagggt atttcgaccg gttcctggcg
      241 gagttcccgg gaacgaagat cgcgatcgcc tacgcgctgc aactcatccc ggaaatgccc
      301 gccgaagccc atgacatacc gatggacatc atcgtgaccg aatcccgcgt gtaccggcgc
      361 ccaaggtgaa gcgatacggc ccgcgaccgg cttacagcag ggtcaggatc tcgtcggcat
      421 agctttcgat cgagcgttcc gggctggcgc tgagccactg aatcgcggga tccgcccgaa
      481 accaggtcaa ctggcgtttc gcgaaccggc gcgtattctg tttcatggag gcgactgcct
      541 cgtccagcgt tttttccccg gcgagaaagg cggcgatctc ccggtacccg agtgaacgga
      601 ggcggtagag gtgtttcgcg tggcccgcgt cgagcagcct ctgcacctcc tcgacgaagc
      661 cctgttcgat catccggtca acccgggcat cgatgcgttc gtaaagctgt tcgcggggcc
      721 agtccagcgc gacctgcacg gcatcgagcg gcaccgcggc catcttgtgc ccggcatgga
      781 gctgcgacag gggatcgccg gtcagttcgt gtacttcaag agcgcggacg atgcggcgca
      841 ggtcgtttga atggatgacc tgggcatagg ccggatcgca ggcctgcaat cgcgcgtaga
      901 gcgcaggcac accggcttct tcagcttccc ggcacaaccg ttcgcggatg gagtcgtctt
      961 ttccgggccc ggggaacagt ccctcgatca gtgcgcggac gtacaaccct gcgcctccgg
     1021 ccacgacagc gatccggccg cgcgcgttca aggcatcgac gagctggcgc gctctccggc
     1081 cgaacaggcc ggccgagaat tcctcgccag gttcgaggag gctgacgaaa tggtgtttcg
     1141 cgcgggcttg ttcctgggcg gtgggcgccg cggtaccgat ctccattccc cggtacactt
     1201 gcatggagtc cgccgagatg atctccgaat ccaggcgccg ggcaagttcc agggcgaggg
     1261 cggtctttcc tgaggcggtc ggcccgacga cggcaaccag attcttcact ggcgcctccg
     1321 gaaactcttt tccatctgca cctgggtcag ttccgtgatg atcgggcggc cgtgcgggca
     1381 ggtgtagggc ggccgcaggc gccgaaaccc ttccaggagt tcccggcgct cctccggcga
     1441 caggcggtcg cctgccttca cggagctgcg gcatgcctcg acggtcaacc ggagcaaatc
     1501 ggagacgagt tcctcgcgat tgaacaaatc accctgggcg agcgtatcca gcacgcgatg
     1561 gacggcgccg ggaatggcgg cgtcttcgta gagatggcag agggcggtca cctggaatgt
     1621 gctgccgccg aaaggttcaa tttcgatgcc cagctttgaa aacagggcgt ggtgctcctc
     1681 cagcagtttc acgtacgaag gcggcacatc gaccaggatt ggcacgacga gctgctgggc
     1741 ctggtaatcg gcgtcctgca ggtctgcgag gagcgtgtcg aaggtcaggc gttcgtgcag
     1801 ggcatgctgg tcgatgatca acaggcggtc ctcttccggc acgagaagat acgtcgcgaa
     1861 aagctgtatc ggcgcctcgc tgatctgtcc gagcgaagcg tagaaggccc ggggctcgac
     1921 accggcagct tcttcgcgcg gtttacgctc cggtaagccc tcaaactccg tctgggcagg
     1981 aggcggcgga tcgagcgccg gctggcgcac cggcgtggac ggtgctggcg tacccctggg
     2041 atcgaaaata gtggtctgcg gttccgggcg gctgatggat gtgttgcgct ggccttcgga
     2101 gtaacgggct cgctcctgtc cggacgccgc atcgtcgtat acaggctctg ccgcaggctg
     2161 cggtgtccgg gtgagcctct gcaggcggtt gcgcgtggcc tcgcggaggg cctggcgggc
     2221 ggcgttctcg tcgcgaaacc ggatctcacg tttcgcgggg tggatattaa catccacgag
     2281 gcgtggcggc gccgtcacga ggacaatgcc caccgggcgc cgcccgacgg taagcagacc
     2341 gcggtagccc tcctcaaaac catattggag gcttctattg accacggggc gcccattgaa
     2401 gaaaaagaac tgatgcgtgc ggccggaacg cgtgagcgcg ggggtgccga taaagcctga
     2461 gaaactcagc cccgcgccgg cgccatccaa tgcgacgagt tcctccgcaa atccgaggcc
     2521 ccagatgagt gcgatacgct ccttcaggct ggtattttgg ggaatatcga agagcagttt
     2581 ttcattgtgc agcagttgaa acgcgacccc gggcgaggcc agggcgtggc gctgcacgat
     2641 atcgatggac tggcttaatt cggtcgtgat gcctttgaga aacttggcgc gcacgggggt
     2701 gttgaaatac agccggttca ccgtaacccg cgttccgggc ggcgcggaga tctgcgacac
     2761 atcgcgcagg acgccccctt caatccggat tcgcgtggca tgctcgtcac tggcccggcg
     2821 agtgaccagt tcaaagcggg acaccgaagc gatactgggc agggcctcac cccggaagcc
     2881 catggtcacg acattatcga tgtcatcggc tttgcggatt ttgctggtgg catggcgttc
     2941 aatggccagt aaggcatctt gctcgctcat gccatggccg t
//
LOCUS       DAOPBQ010000479         2979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26224_length_2979_cov_4.062585, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000479 DAOPBQ010000000
VERSION     DAOPBQ010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26224_length_2979_cov_4.062585"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1759
                     /gene="ccsA"
                     /locus_tag="PKW60_13120"
     CDS             <1..1759
                     /gene="ccsA"
                     /locus_tag="PKW60_13120"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPK00421.1"
                     /translation="MLAGMTHPDLGPVEWLMDTLFYPRAAAAYPVFLVHSDEVAEAIG
                     VPHQGKKKRDRYSYLELLPGRRTLFQLADQYAARPERELTGVQRQIIHLAHNLADYES
                     LLSFFDFAREEYPTRATEGLRLIFPEESQTRYSDVLRRAPLLASFIHTLNAAEGGELS
                     SEKREAELTALRELLGQIDHFGLSAKGIAMLPPLGNGFSEQWISPGDVVTEAFMSEQA
                     PETHLALTELLEDLAAAVDDRTAFSAYLRGLHDELVSRATERGEYGKVPLEVTFYRYR
                     LIGRSLPLFILCFMLVALTWLKPGWRRLVFAAPYALAVPTLLLSAGIAMRCIIRARPP
                     VTTLYETILFTTAVCVALAIAIELMNRRRIAVSLGAVLGAAGMFLANRYELKEGVDTM
                     PSMIAVLDTNFWLSAHVTTIVMGYGAALLGAAIGHVYILGRVLRIRNSDEDVYAGIYR
                     MTYGVMAFGLVFAVIGTVLGGIWANESWGRFWGWDPKENGALMLVLWMLVLFHARLGG
                     YIRGLGFNVGAVACGMVVAFSWWGVNLLGVGLHSYGFTTGIFRILAGFYVIETVVLLL
                     GCFVWLRDRGYLAPARPKA"
     gene            1865..>2979
                     /locus_tag="PKW60_13125"
     CDS             1865..>2979
                     /locus_tag="PKW60_13125"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00422.1"
                     /translation="MTRQLDRREFLTSGSRAGAGMVLAGAALAQTDAPQPQPQSEGAP
                     VRVGLVGYGKRGRSLISTLNALGPDVEILAVCDVLPERVAEAQAALERMGRPKPAGYA
                     QGEEDFRRLCEQQDLDLVMNAAPWDWHVPICVAAMNAGKHTATEVPAAVTVDGCWQLV
                     DTAEATGKHCVLLENCCYFRNAMMVLNMVRKGLLGEMLHAEAGYQHYRIANNVFDAEG
                     NLLWCGEHESRRDGCLYPTHQVGPVGQWMNITRGDQFAYLVSMSSKSRGLNIYAARHF
                     GPEHPLATRPYALGDINTTLIRTENGLTITLYHDTQAPRPYDLIYRAQGTNGLYMGTL
                     DKIFIEGRSKSEEWDDIEPYLEEYEHPLWKALQQNALG"
BASE COUNT          523 a          876 c          994 g          586 t
ORIGIN      
        1 tatgctcgct ggcatgaccc atcccgacct ggggccggtc gaatggctca tggatacact
       61 gttttacccg cgcgcggcgg ccgcgtatcc ggtatttctg gtccattccg acgaagtggc
      121 ggaagccatc ggcgtgccgc accaggggaa aaagaaacgc gaccgctatt cgtatcttga
      181 gctgctgccg gggcgccgca cgctgttcca gctcgccgac cagtatgcgg cgcgtcctga
      241 gcgtgaattg acgggagtgc agcggcagat catccatctc gcacacaacc tggcggacta
      301 tgaatcgctg ttgtcgtttt ttgattttgc ccgcgaagaa taccccaccc gggccaccga
      361 aggtctgcgc ctgattttcc cggaagaaag ccagacccgc tatagcgatg tgctgcggcg
      421 ggcgccgctg ctcgcctcgt tcattcacac cctgaacgcg gcagagggcg gagaactcag
      481 ttcggagaaa cgcgaggccg agctgaccgc cctgcgggag ctgctggggc agatcgatca
      541 tttcggcttg tctgcgaaag gcatcgcgat gttgccgccg ctgggcaatg gttttagcga
      601 gcaatggatt tcaccggggg atgtggtgac cgaagccttc atgtccgagc aggcccccga
      661 gacgcatctt gcgctgacgg agcttctcga agacctggcc gcagcggtgg atgatcgcac
      721 cgcgttcagc gcgtatctgc gcggattgca cgacgaactc gtgagccggg ccacggagcg
      781 gggggaatac ggcaaggtgc cgctggaggt gaccttttac cggtaccgct tgatcgggcg
      841 aagcctgccg ctattcatcc tgtgtttcat gctggtggcg ctcacgtggc tcaaacccgg
      901 ctggcggcgc ctggtttttg cagcgccgta cgcgctcgcc gtgcctacgc tgctgctgtc
      961 agcgggcatt gccatgcggt gcatcatccg cgcacggccg ccggtcacta cgctgtatga
     1021 gacgatcctg ttcaccaccg ctgtgtgcgt ggcgctggcg atcgccatcg agctgatgaa
     1081 ccgccggcgc atcgccgtat cgctgggcgc ggtcctgggc gcggccggca tgtttcttgc
     1141 caaccggtat gagcttaagg aaggcgtcga caccatgccg agcatgatcg cggtgctcga
     1201 taccaatttc tggctgtcag cgcacgtcac gacgatcgtc atggggtacg gagcggctct
     1261 gctcggggcg gccatcggcc atgtgtacat cctgggccgg gtcttgcgga ttcgaaacag
     1321 cgatgaggat gtctacgcgg gcatttaccg catgacctat ggcgtgatgg cgttcgggct
     1381 ggtgtttgcc gtgatcggga cggtgctcgg cggcatctgg gccaacgaga gctggggccg
     1441 cttctgggga tgggacccca aggaaaacgg cgcactcatg ctcgtgttgt ggatgcttgt
     1501 gctttttcat gcccggctgg gcggctacat ccgcgggctg gggttcaacg tcggcgcggt
     1561 ggcctgcggc atggttgtgg cattttcatg gtggggggtg aaccttctcg gcgtcggatt
     1621 gcacagttac ggcttcacca ccggcatttt tcggattctg gcgggatttt acgtgatcga
     1681 gaccgtagtt ctgcttctgg gatgttttgt gtggctgcgc gaccgcggtt atctggcgcc
     1741 cgccaggcca aaggcgtgat ctcgccggat tgtcgcggcg tattgaccct gacgcaaaat
     1801 accggtacgt taggcgcggg aatgcctgcc gaacaggggg cttttaccat aaaggagttt
     1861 ggcgatgaca cgacagcttg atcgacgcga attcttgacg tccgggtcac gggcgggcgc
     1921 aggcatggtg ctcgcgggtg cggcgctggc tcagacggat gcgcctcagc cccaacctca
     1981 atccgagggc gcgcctgtgc gcgtcgggct ggtgggttac ggcaaacggg gccgttcgct
     2041 catcagcacg ctgaacgcgc tggggccgga cgtggagatc cttgcggtct gcgacgtgct
     2101 gccggagcgg gtcgccgagg cgcaggcggc gctggaacgc atgggacgcc ccaaacccgc
     2161 cggttacgcg cagggcgagg aggatttccg gcggctgtgc gagcagcagg acctggatct
     2221 cgtgatgaat gccgcgccct gggactggca cgtgccgatc tgcgtggcgg ccatgaacgc
     2281 gggcaaacat accgcgaccg aggtgcctgc cgcggtgacc gtcgatggat gctggcaatt
     2341 ggttgataca gctgaagcaa cgggcaagca ctgcgttctt cttgagaatt gctgctattt
     2401 ccgcaacgcc atgatggtct tgaacatggt gcgcaaaggg cttctgggcg agatgcttca
     2461 cgcggaagcg ggctatcagc attaccggat agccaacaac gtgttcgacg cggaaggcaa
     2521 cctgttgtgg tgcggggagc acgagtcgcg gcgcgacggc tgcctctatc ccacccacca
     2581 ggtcggcccg gtgggccagt ggatgaacat cacccgtggc gatcagttcg cgtacctggt
     2641 gtccatgagc agcaaatcgc gcggactcaa tatttacgcg gccagacatt tcggaccgga
     2701 gcatcctctg gcgacgcgcc cctacgccct cggcgacatc aatacgaccc tgatccgcac
     2761 cgaaaacggg ctgaccatca ccctgtatca cgatacgcag gcgccgcgcc cctacgacct
     2821 catctaccgc gcgcagggca ccaacggcct gtacatgggc acgctggaca agatcttcat
     2881 cgagggccgc agcaagagcg aagagtggga tgatatcgag ccctacctcg aggaatatga
     2941 gcatcccctg tggaaggcgc tgcagcagaa cgcgctcgg
//
LOCUS       DAOPBQ010000480         2970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26305_length_2970_cov_5.623671, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000480 DAOPBQ010000000
VERSION     DAOPBQ010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26305_length_2970_cov_5.623671"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2131)
                     /locus_tag="PKW60_13130"
     CDS             complement(<1..2131)
                     /locus_tag="PKW60_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00423.1"
                     /translation="MISPIVRLFVLTLPALFSCAYAAMPDASATLSQRTEPTDDGPRI
                     VQDVTFETASAGYTLRCEYLEREDDPARIGFSKWAPTIGYVPVGIAKPSMENWYNQGF
                     FQWTFDGFNINEFKPQFRIIREFGEDAMVEYIWDTPKVTAIARFAVTSRSDKLLFLGR
                     YEPKEEIRDVRLRLMAYPATFEKPWNRRVTTQMRTLAEGAADIDLEQERWLLFEDVEP
                     GRTGTGSAGLLLGDASAFARVAVSAIGGYAEYTDITLSPGRRDFVLGFYEDPSLQDFE
                     AVRAYFRRSASAESDAIAALASADWDQPLPLLPVDEEQLAQVRSEDEAQLNRPAELWR
                     PRPEPLEFPWAVNLPGGPIRAALLSPRWIAYDTMELARRLALDVRHQYFDAADAIATP
                     NVWYYRAHTGIGPLNPGLAMRNAANICLDPEREVIVVAKLKTEAIGPRLQPIILEQIR
                     NGKGLIITGGADATAGWPEDLFADEDTEFAAKLLSYFPWEKMPSLRKDSRGRLGDAPP
                     LRTFRYGEGRVFVFQAKLANYSALLPLNSLKQGLDGADDRLLALHALLWTAAAKRPLP
                     SRITFGDSLAPFMAATANELPVDFSGAEFTQVRVRIQDETDSVQVLSDDLLDETGTYL
                     RIPPLPAMHAYYLDIVALNASSECVGLGGTILQIAPEYTLASLAVTPSGQNHEAAPPR
                     VDLPEGGPLTLTAAVTPAPGDETLRVEF"
     gene            2337..>2970
                     /locus_tag="PKW60_13135"
     CDS             2337..>2970
                     /locus_tag="PKW60_13135"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HPK00424.1"
                     /translation="MTFTLYFLTFCASLSGSAAPANAPAPSERSESSLELWYPQPADE
                     WVEALPVGNGRLGGMVFGGVLNERIQLNEETVWAGSCQTYADKPDGARTIEEARRLFF
                     AGRYAEGEELIQREIMNKHITPRSYQTLGDLRLVMKDPGHTASDYRRSLDLDTATATT
                     QFTMDGVTYTREVFASVADDVLVVRITASQPGALDLEIALDRPADSVTSVL"
BASE COUNT          561 a          863 c          982 g          564 t
ORIGIN      
        1 cgaattccac gcgcagtgtt tcgtcgccgg gcgccggcgt gacggcagcc gtgagggtca
       61 gtgggccacc ctccggcaga tccacccgcg gcggcgcggc ttcgtgattt tgaccggacg
      121 gggtcactgc gagcgaggcc agggtgtatt ccggggcaat ctgcaggatc gtgcctccaa
      181 gcccgacgca ttcgctggac gcattcagtg ccacgatgtc gagataatag gcgtgcatgg
      241 cgggcagagg agggatccga agatacgtcc cggtttcatc aagcagatcg tcgctgagaa
      301 cctgcacaga gtctgtctca tcctgaatgc gtacgcgcac ttgagtgaac tcagcgccag
      361 agaaatccac cggcaattca ttcgcggtcg ccgccataaa cggcgcgagc gagtcaccga
      421 acgtgatgcg ggagggaagt ggccgtttcg ccgcggcggt ccagagcagt gcatgcaggg
      481 cgaggaggcg atcatcagcg ccgtccagcc cttgcttcag cgaattgagc ggcagcaggg
      541 cgctgtaatt ggccaatttg gcctggaaca cgaacacacg tccttcgccg tagcggaagg
      601 tgcgtaacgg gggcgcgtcg cccaggcgtc cgcggctgtc ctttcggagg ctgggcatct
      661 tctcccaggg aaaatacgac agaagcttcg cggcgaactc cgtgtcttcg tccgcgaaca
      721 gatcctctgg ccacccggcg gtggcgtctg cgccgccggt gatgatgagc cctttaccgt
      781 tgcgaatctg ctcgaggatg attggttgca gacgcgggcc gatggcttca gtcttcagct
      841 tcgctacgac gatgacttcc cgctcaggat ccaggcagat gttggcggcg tttcgcatgg
      901 ccaggccggg gttgagcggc ccgatgccgg tgtgcgcccg gtaataccaa acattgggcg
      961 tggcgatggc gtccgcggcg tcgaaatatt gatgccgcac gtcaagtgcc aggcggcgcg
     1021 caagttccat ggtgtcgtag gctatccatc ggggcgacag aagtgccgcg cgaatcggtc
     1081 ctccgggcag atttacggcc cacggaaatt cgagcggctc gggccgggga cgccagagtt
     1141 ccgctggacg gttgagctgg gcctcgtctt ctgaacgcac ttgagccagc tgctcttcat
     1201 cgacgggcag gagtgggaga ggctggtccc aatccgcgct ggccagggcg gcgatggcat
     1261 cagattcggc gctggcactc cggcggaaat acgcacggac ggcctcaaaa tcctggaggg
     1321 acggatcttc gtagaatccg agaacaaaat cacgccggcc ggggctgagg gtgatgtccg
     1381 tatactcggc atagcctccg atcgcgctga cagccacgcg cgcgaaagct gaggcatcgc
     1441 ccagcagcaa tcccgctgat cccgtgcccg tccgcccggg ttcaacgtcc tcgaacaaga
     1501 gccagcgttc ctgctccagg tcgatgtcag cggcgccttc cgcgagggta cgcatttgag
     1561 tggtcactcg ccggttccag ggtttttcaa aggtggccgg gtacgccatc agacggagcc
     1621 ggacgtcgcg gatttcctcc ttcggctcat aacggccaag gaagagcagt ttgtcacttc
     1681 gcgaagtgac ggcaaaccgc gcaatggccg tgactttggg cgtgtcccag atgtactcga
     1741 ccatggcgtc ctcgccgaat tcccggatga tccggaattg tggtttgaac tcattgatgt
     1801 tgaatccatc gaaggtccac tggaaaaagc cctggttgta ccagttttcc atggagggtt
     1861 tggcaatgcc cacgggtacg tatccgatgg tgggcgccca tttcgagaac ccgatgcggg
     1921 cgggatcatc ctcgcgttcg agatattcgc agcgcagtgt atatccggct gaagcggttt
     1981 cgaaggtcac gtcctgaacg atgcgcggcc cgtcatccgt gggctccgta cgctggctca
     2041 gtgtggcgct ggcatccggc atcgcggcgt acgcgcagga aaacagggcg ggcagtgtca
     2101 gaacgaaaag ccggactatc ggactgatca tcgtatcatc tcctccggga tatggcggga
     2161 agcatcgcgc tgcgtgtgca ttttgtgagt gtgcccgaat tgaaatcccc tgggcaagtt
     2221 cgtaatgtga cggccgcagc ccgggtggaa ggcaggtacg aatgctcgaa gccgcccggg
     2281 ccgcgagaaa ctgcaaaccg gggcgcgaaa ccatttagcg tggatttgag gccgttatga
     2341 cattcactct gtattttctg acgttttgcg cttccctgtc cggttcggcg gcgcctgcga
     2401 acgctcctgc gccatcggag agaagcgagt cctccctgga gttgtggtac ccgcagcccg
     2461 cggacgaatg ggtggaggcg ctgcccgtgg gcaacggccg cctgggcggc atggtgtttg
     2521 gcggggtgct caacgagcga atccagctca acgaggagac cgtctgggcc ggtagttgtc
     2581 agacatacgc cgacaaaccg gacggagccc gcacgatcga ggaggcgcgc aggctgtttt
     2641 ttgcggggcg gtacgctgag ggcgaggaac tcatccagcg cgagatcatg aacaagcaca
     2701 tcacgccgcg cagctaccag acgctgggtg acttacggct tgtcatgaaa gacccggggc
     2761 acacagcctc ggattaccgc cgaagcctgg atcttgatac ggcaactgcg acgactcagt
     2821 tcacaatgga cggcgtcacg tacacgcgcg aagtctttgc gtcggtggcg gacgacgtcc
     2881 tcgttgtgcg gattacggcg tcccagcccg gcgcgctcga cctggaaatt gcgctggatc
     2941 gcccggcgga ttccgtgacc agcgtgctgg
//
LOCUS       DAOPBQ010000481         2926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26802_length_2926_cov_3.621735, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000481 DAOPBQ010000000
VERSION     DAOPBQ010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26802_length_2926_cov_3.621735"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1187
                     /locus_tag="PKW60_13140"
     CDS             96..1187
                     /locus_tag="PKW60_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00425.1"
                     /translation="MRRLSLFAPLGNALLAILLTCLFAACGNIVDKDRIRIAKVGTTY
                     ITRGDLYRYLRQMAPEERPHINNRGDLLKVLEKYIDNELQKLVLASSLAENKIQPLSE
                     ERLAQFYDAQHPEMSISIEQLEEFGMNQADYEALQLRRQKGIDELRENVMRREAIILA
                     IQEAVEKGEITITNEEYAAEYEMRKGGLFNPERATFRGLVFPVQTPDAGPNSAEARKR
                     LVKGDPWADVAASFVDAHKAIPLQTTFVNMGQPKFQSFWEQASGTQKGRILGPMIIED
                     WEMVLQGSGNDAQSANLTGYLVAEILEVNPATQMTLEEAEEELAPAIYFVKYMKALRE
                     KSGVEIYEDHLPEPGMFREKTSDPLIESL"
     gene            complement(1286..>2926)
                     /locus_tag="PKW60_13145"
     CDS             complement(1286..>2926)
                     /locus_tag="PKW60_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPK00426.1"
                     /translation="MSSENSFRDAVRDWTRLLGEENVRTDEETLAWYSRTTQDTAPRP
                     SCVVYPKSTEQVQELVRLATRYGVVLYPISRGRNWGYGDACAPTPEAVIVDLSRMDQI
                     LEVNTELAYAVIQPGVSQGQLYAYLAEHQTGLWMDSTGAGPDSSLVGNTLDRGFGHTR
                     YGDHFETCCGMEVVLADGRVLETGFGHFANAKAGRVYRYGVGPFLDGLFCQSNYGIIT
                     KIGLWLMPEPEDFCFFFCTVKNDDGLERLIEALRPFRLRRMLDSAIHIGNDLRVLSSR
                     IHYPWESAGQAGALSERFRQQLRKTHGIGEWSVGGALTGTRGHVRDMRRLLRRALGPL
                     GKLIFVDDAKLALGWRVARLAERFGMTTLSEQLASLRPVYAQMKGAPTDESVHGTQWR
                     LRQPPDHDSDPRDPLESGCGLMWFSPVVPMTGRDARRVLTLVKPVLAEHDFDLLATFT
                     LLNERAMIGILNVAFDKAVPDEVERAMACYDDAMRVLIDDGYVPYRTGLRGMDKIRDE
                     DDVFWQVAREIKQVLDPGDIIARGRYVPPLDERDPAGF"
BASE COUNT          657 a          918 c          811 g          540 t
ORIGIN      
        1 catccgctcc gggcatgcgc aatccaggca atccgcgcag ctttctccgc gccgcctgtt
       61 cgtgctagac ttccgcaatg ctaaaggagg ctttcatgag gcgtttatcc ctatttgcac
      121 cgctgggcaa tgcattactg gccattctgc tcacctgttt gttcgcagca tgcggcaaca
      181 tcgtggataa ggaccgcatt cgcatcgcca aagtgggtac aacgtacatc acccgcggcg
      241 atctgtaccg atacctgcgg cagatggcgc cggaagaacg tccccacatt aacaaccggg
      301 gcgatttgct caaggtgctc gaaaaataca tagataacga gctgcagaag ctggtcctcg
      361 cttcatcact ggccgagaat aaaatccagc cgctgtccga agagagactc gcccagttct
      421 acgacgccca gcacccggaa atgagcatct ccatcgaaca actcgaagaa ttcggaatga
      481 accaggccga ttatgaggcc ctgcaactga gacggcagaa aggcatcgat gagcttcggg
      541 aaaatgttat gcgccgtgaa gccatcatcc tggcaatcca ggaagccgtt gaaaaaggcg
      601 aaattaccat caccaacgag gagtacgcgg cagaatacga aatgcgcaaa ggcgggctgt
      661 tcaatcccga gcgcgcgact ttccgcggcc tggtctttcc ggtgcagacg cctgatgccg
      721 gccccaattc cgcggaagcc cgaaaacgtc tcgttaaagg agacccctgg gccgacgtgg
      781 ccgcatcgtt cgtcgatgcg cataaagcca ttcccctgca gacgacgttc gtcaatatgg
      841 gacaacccaa gtttcaatcc ttctgggagc aggcttcggg aacgcaaaaa gggcgtattc
      901 tgggcccgat gattatcgaa gactgggaga tggtgctcca gggcagcggc aatgacgctc
      961 agagcgccaa tctcaccgga tatctggtgg ccgaaattct cgaagttaat ccggcaaccc
     1021 agatgaccct cgaggaagcc gaagaagagc tggctccggc aatctatttc gtcaaataca
     1081 tgaaggcact gcgtgaaaaa tccggggtcg aaatttacga agaccacctt ccggaacccg
     1141 gcatgtttcg cgaaaaaaca agcgacccgc tcatcgaatc cctttaattg ccggccttaa
     1201 tacccttctt ccgctacgcg ggccacggcc ggaaaccagg gcggcggcgc tgtcattcag
     1261 gcaggcatcc tgatctcgcc gcgcttcaaa atccggcggg atcgcgttcg tcgagcggcg
     1321 gcacatagcg cccccgggca ataatatcgc ccgggtcaag tacctgctta atctcgcgcg
     1381 ccacctgcca gaagacatca tcctcatccc gaatcttgtc cataccgcgc agccctgtcc
     1441 ggtacggcac atagccgtcg tcgatcagga cccgcatggc atcgtcgtag caggccatgg
     1501 cgcgttcgac ttcgtccggg acggctttat cgaaggccac gttgagaatc ccgatcatcg
     1561 cgcgttcatt tagaagggtg aacgtggcca gcagatcgaa atcgtgttcc gccaggaccg
     1621 gtttgacgag cgtcagcacg cgccgcgcat cgcgcccggt catcgggaca acgggcgaga
     1681 accacatcag cccgcatccg ctttccaggg ggtctctggg gtcgctgtcg tggtcgggag
     1741 gctgccgcaa gcgccattgc gtgccgtgga ccgactcgtc cgtcggcgcg cctttcatct
     1801 gggcgtagac cggccgcagc gaagccagtt gctcggacag cgtggtcata ccgaaccgtt
     1861 ccgcgagccg cgcgacacgc cagccgaggg cgagtttcgc gtcatccacg aagatgagct
     1921 tgcccagagg gcccagtgcg cggcgcagca ggcggcgcat gtcgcgcaca tgcccgcgcg
     1981 tccccgtcaa cgcgccgccg acgctccatt caccgatgcc atgcgtcttg cgtaattgct
     2041 gccggaaacg ctcggaaaga gctcctgcct ggcctgccga ttcccaggga taatgaatcc
     2101 ggctgcttaa tacacgcaaa tcgtttccga tatgaatggc cgagtcgagc atacgccgca
     2161 accggaacgg gcgcagtgct tcgatcagcc gctccagacc gtcatcattc ttcacggtgc
     2221 aaaagaaaaa gcagaaatct tcgggctccg gcatgagcca gaggccaatc ttcgtgatga
     2281 tgccataatt ggattgacag aaaagcccat caagaaacgg acctacgccg tagcggtata
     2341 cgcgtccggc tttggcattc gcaaaatggc cgaagcccgt ctcgagcaca cggccatcgg
     2401 ccagaacgac ctccatccca caacaggtct caaaatgatc gccgtagcgc gtatgcccga
     2461 aaccccggtc gagagtattg cccaccaggc tggaatcggg gccggccccg gtgctgtcca
     2521 tccacaatcc ggtctggtgc tccgccagat acgcgtacaa ttgcccttgc gataccccgg
     2581 gctgaatcac cgcatacgcc agctcggtat tcacctcgag aatctggtcc atacgcgaca
     2641 gatcgacgat caccgcctcc ggcgtcggcg cacaggcgtc accgtagccc cagttccgcc
     2701 cccgcgaaat gggatacagt accaccccgt agcgggtggc gagccgcaca agctcctgca
     2761 cctgttccgt ggatttggga taaacgacgc aactggggcg gggcgcagtg tcctgcgtgg
     2821 tgcgagaata ccacgcgagg gtttcctcat ccgtccggac attttcttca cccagcagcc
     2881 gcgtccagtc tctcaccgcg tcgcgaaacg aattttcaga agacat
//
LOCUS       DAOPBQ010000482         2924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26820_length_2924_cov_4.917393, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000482 DAOPBQ010000000
VERSION     DAOPBQ010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26820_length_2924_cov_4.917393"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKW60_13150"
     CDS             complement(<1..300)
                     /locus_tag="PKW60_13150"
                     /inference="COORDINATES: protein motif:HMM:NF019931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HPK00427.1"
                     /translation="MTPHQDRAQRSAAIKARAAELGFDACAVAAAEPVDPDDRLGTWL
                     ASGFHADMDWMVRTKALRQDVRFKVPGARSVVVVARNYHAPRPPKPPDTGRVSRYA"
     gene            complement(305..1033)
                     /locus_tag="PKW60_13155"
     CDS             complement(305..1033)
                     /locus_tag="PKW60_13155"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPK00428.1"
                     /translation="MLEFFAVGRDLLDKVEQTQAAAIREAAAMIAESLMGGGVWHVFG
                     TGHSHFLGEEVYYRAGGLAPVNAILFPALMQHEGPATSTRLERTPGLARIILDKAGPR
                     PGEVLTIVSNSGKNAVPVEMALYARELGLKTVALTSLNQSTAASLGAGQKQKLYEICD
                     IVIDNCGSAGDAALQVPGTERMTAATSSFVGIAIMEQIVYLVCCAYAEAGIEPPVFKT
                     ANLPGGDEWNARLIEQYGGRVNLR"
     gene            complement(1072..>2924)
                     /locus_tag="PKW60_13160"
     CDS             complement(1072..>2924)
                     /locus_tag="PKW60_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HPK00429.1"
                     /translation="VRKAPHTWGWDIMPRAVSAGIWRGVRLEPRPAYGFEQIYYRTLE
                     ADARMAVLTAMFQVRAPDPDLLGCAVEFEGVCGDHRFEYAWPLEFAADSCRIYVPDAR
                     LWWPKGYGDPNLYAVTARLVRGGEVLAQRSDRIGVRTVTLERSASAGIPHALAARGLD
                     VCRYDEPPDPAGHFMFRVNDVPIQVRGTNWVPLDAFHSRDAGRLPDAMTLADDLGCNM
                     IRCWGGNVYEDQAFFDFCDEHGILVWQDFAFACCRYPQNEAFLERVRSEAEAVITRLR
                     NHASLALWCGDNEIDECYASDGLSPAHNRISREVLPQVVHRCDPGRCYVPSSPYRAPE
                     AQSELPEQHLWGPRGYFKSPYYTRHTAGFIGEIGYHGCPNAGSIRRFIEEDAWWPWED
                     NAQWRVHDVSHLQHNAADRNRIKLMANQIREYFGDVPSTLETFALASQIVQAEAKKYF
                     IESTRQRKWRTSGILWWNLLDGWPQFSDAVVDYYFEKKLAYWYIRRVQRPVCVMLGDA
                     REGKYLPVIVANDTRHDAAVRFEVADPAGEILYAGEVSVPAAQNWQVGRIRTYASDRA
                     LYLLRWEINGEAFGNHYIAGSPPFLLDDYRGWLTHIAALPQAFTAETVGQ"
BASE COUNT          526 a          973 c          902 g          523 t
ORIGIN      
        1 ggcgtagcgc gacacccggc ccgtgtccgg cggttttggc gggcggggcg catggtaatt
       61 gcgcgccacg accactacgg agcgcgcccc ggggactttg aagcgcacat cctgccgcag
      121 tgccttcgtg cggaccatcc agtccatatc cgcatgaaac ccgctggcga gccacgtgcc
      181 caggcgatca tccgggtcaa ccggttcagc agccgctacg gcgcacgcgt cgaatcccag
      241 ctccgccgcg cgggccttga tcgctgcgct gcgctgcgct ctgtcctggt gaggcgtcat
      301 gggcttatcg aaggttcacg cggccgccgt attgctcgat caggcgcgcg ttccactcgt
      361 cgccgccggg aagattcgcg gtcttgaaca cgggcggctc gatgcccgcc tccgcatagg
      421 cacagcagac gaggtagacg atctgctcca tgatggcaat gcccacgaaa ctggacgtcg
      481 ctgccgtcat tcgttcggtt cccggcacct gcagcgcggc gtctcccgcg gacccgcaat
      541 tgtcgatgac gatatcgcag atctcgtaga gcttctgttt ttgtcccgct cccagagaag
      601 ccgccgtgga ctggttgagc gacgtgagtg ccacggtttt cagccccagt tcccgggcgt
      661 agagggccat ttccaccggc acggcgttct tgccgctgtt ggagacgatg gtcaatacct
      721 cgccggggcg cgggccggcc ttgtcgagga tgatccgggc cagtcccggc gtgcgctcga
      781 gccgggtgct cgtggcaggc ccttcgtgct gcatcagcgc gggaaacagg atcgcgttca
      841 cgggcgccaa gcctcccgcg cggtaataca cttcctctcc gaggaaatga gaatgccctg
      901 tcccaaacac gtgccacacg cccccgccca tgaggctttc ggcaatcatc gcggcggctt
      961 cccgaatggc cgcggcctga gtctgctcca ccttgtccag caggtcacgt cctaccgcga
     1021 aaaactcgag catcacagcc tcctttgtcc ggcagcgttc tgcgtgcggc gctactgccc
     1081 cacggtctcg gccgtaaacg cctgcggcag cgcggcgata tgcgtcagcc agccgcggta
     1141 gtcgtccagc aagaacgggg gagagccggc gatgtaatga ttgccgaacg cttcgccgtt
     1201 tatctcccag cgaagcaggt acaatgcgcg atcgctggcg taggtgcgga tgcgtcccac
     1261 ctgccagttc tgtgccgccg ggacgctcac ttcgcccgca tacaggattt cgcccgcggg
     1321 atcggcgacc tcgaaccgaa ccgcggcatc atgacgcgtg tcgttcgcca cgataacggg
     1381 aaggtacttg ccctctctgg catcgccgag catgacgcag accggtcgtt gcacccgccg
     1441 gatataccag taggccagct tcttttcaaa atagtagtcg accaccgcgt cggagaattg
     1501 cggccaccca tcaagcaggt tccaccacaa aatcccgctg gtccgccatt tgcgctggcg
     1561 cgtagactca ataaaatatt tcttcgcctc cgcctgcacg atctggctcg ccagcgcaaa
     1621 ggtctcgagt gtggacggga catcgccgaa atactcgcgg atctggttgg ccatgagctt
     1681 gatccggttg cggtcggccg cattgtgctg caagtgcgat acgtcatgga ctcgccactg
     1741 cgcattgtcc tcccacggcc accaggcgtc ttcctcgatg aagcgccgga ttgacccggc
     1801 gttcgggcag ccgtggtatc cgatctcccc gataaatccc gccgtgtgcc gggtgtaata
     1861 cggactctta aaatagcccc gcggccccca gaggtgctgc tccgggagct cgctttgcgc
     1921 ttcgggcgcg cgatacggcg aactgggaac gtaacagcgt ccgggatcgc agcgatgcac
     1981 cacctgcggc agcacttccc gggaaatccg gttgtgtgcc ggagaaagcc catccgacgc
     2041 gtaacattcg tcgatctcat tgtctccgca ccacaacgcc agggatgcat ggttgcgcag
     2101 gcgcgtgatc accgcctcgg cctcggaacg gacacgctcg agaaacgcct cattctgcgg
     2161 ataccggcag caggcgaaag caaaatcctg ccagacgagg atgccgtgtt cgtcgcagaa
     2221 atcgaagaag gcctgatcct cgtacacgtt gccgccccag caccggatca tgttgcagcc
     2281 gagatcgtcc gccagtgtca tggcatcggg cagacgccct gcgtcgcggc tgtggaaagc
     2341 atccagggga acccaattgg tcccgcgcac ctggatgggc acgtcattga cgcggaacat
     2401 gaaatgcccc gcggggtcgg ggggctcgtc gtaacggcac acgtcgaggc cccgggcggc
     2461 cagggcatgg gggatccccg cgcttgcgct gcgttccagg gtgactgtgc gcactccgat
     2521 gcgatcgctg cgctgcgcga gcacttcgcc gccgcgcacc agacgcgcgg tgactgcgta
     2581 aaggttggga tccccatagc ctttcggcca ccacaggcga gcatcaggca cgtaaatgcg
     2641 gcaggagtcg gccgcgaact ccagcggcca ggcatattcg aagcggtggt cgccgcagac
     2701 cccttcaaat tcgaccgcgc agccgagcaa atcgggatcc ggcgcccgca cctgaaacat
     2761 cgctgtcagc acggccatcc gggcatcggc ctcaagagtg cggtagtaga tctgctcgaa
     2821 accataggca gggcggggtt ccaggcgtac gccgcgccag atccccgccg aaaccgcgcg
     2881 cggcatgatg tcccagcccc acgtatgcgg cgcttttcgg acat
//
LOCUS       DAOPBQ010000483         2924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26824_length_2924_cov_3.378181, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000483 DAOPBQ010000000
VERSION     DAOPBQ010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26824_length_2924_cov_3.378181"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2580)
                     /locus_tag="PKW60_13165"
     CDS             complement(<1..2580)
                     /locus_tag="PKW60_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00430.1"
                     /translation="MNRIETENGIMSRFRVALSCVLIGLVLQPLWAQDQTGLQPGWTE
                     RDRVEVSTTKAFSNGVDSVTVKLAVESRGPSLLAWGDALFVGHGEHPVTLQHSYPDVL
                     PRDAEITQAVMSADFISWTTAVELDAKVSELGTYFSSVFAASGSNSGSVTISNAMNIS
                     RAVVFLEYRMAEGQPIQTFNDCFEGRTYNLNELTDSPDLLNLTFRTKATTYDPFNENA
                     TMPAPGVQFRARCKAVLRPSYEEVTYYLTPWFETDKQGEVEIAVGSGGAVIETVRDAY
                     VAPPFAALTVVGDGVGYGECRMRIEDLYGFKGKLKIQVKDPLGRPVSGARLELQGIVS
                     AFEYSDSAGELEVEVNGGGTDTRTEEVTVAPRMTISSALTQLVDDVYPHQLHSQSPAY
                     YVAKFAIDNRAVNYMSGRDAFGDFTLNIYENDVLAAEIRGLNAKCAEALTPDDHAQSV
                     NAYFYGVMPEASTKRYRHQLVWKDALGEVPISFATGDRSISEQRIPARERPFRVHYVG
                     IDTAGAYKARGRGIMATWASPGRLAKLNDYFRMMFPGPVEFTYGPDVRMSEPWYTFGP
                     YKVLSYFVDLERHRKSLPDRPDLIVGVSAPGVFGADGLSEPRFREVILIDGCSARESY
                     LLHEYLHTLGLFDNYEYETGEGTRGASANGLDPRTRRRIYNAPGISKPPHQTIMYDRA
                     PDPWLTQDAYSTLLDEATMAIVDLSAHEGADEKAGQLPTASAERQEVFLIGGTFFGRQ
                     SWSDYNYSLRNVWPIFVDTDEVWQPQETVSPSARAVGIRLSNGQRASVLENRLWIGAE
                     DPEGDGELAAAPLFKIPYDPTVNSVTFESYHHGDITRTVREIPFSPNAPTVRIVSPAA
                     GTGL"
BASE COUNT          616 a          966 c          728 g          614 t
ORIGIN      
        1 caatccggtt ccagccgcgg gagaaactat ccgaactgtc ggcgcattcg gggaaaacgg
       61 gatttcccga acggtacggg taatatcccc atggtggtag gactcaaagg ttacggagtt
      121 gacggtggga tcatacggaa tcttgaacag cggcgcggcc gccagctcgc catcgccttc
      181 cggatcttcc gcgccaatcc atagccggtt ttccagcact gacgcccgct gaccgttcga
      241 tagccgaatc ccgacggccc gcgccgacgg cgacaccgtc tcctgcggct gccagacctc
      301 gtccgtatcg acaaagatcg gccacacgtt acgcaacgaa taattgtaat ccgaccagct
      361 ttggcggccg aaaaacgtcc ccccgatcag gaagacttcc tgccgctcgg cgctggccgt
      421 cggcagttgc ccagccttct catcggcgcc ttcatgcgcc gagagatcaa caatcgccat
      481 cgtcgcctcg tccagcaggg tgctgtaggc atcttgcgtc agccatggat ccggcgcccg
      541 gtcatacatg atggtttggt gcggcggctt gctgatcccc ggcgcattat aaatgcggcg
      601 cctcgtgcgt ggatcaaggc cattcgcgct cgcaccgcgg gtcccttctc cagtctcata
      661 ctcatagtta tcgaataggc caagcgtgtg tagatactcg tgcagcagat agctttcgcg
      721 cgccgagcag ccatcgatca ggataacttc acgaaaacgc ggctccgaaa gcccgtccgc
      781 accaaacaca ccgggggccg atacgccgac gatcaggtcg ggccggtcgg gcaacgactt
      841 gcgatgccgc tccaggtcca caaaatacga cagcaccttg tacggaccaa acgtgtacca
      901 cggctcgctc atccgcacgt caggcccgta agtaaactcg accggaccag ggaacatcat
      961 tcggaaataa tcattcagct tggccaggcg tcctggcgag gcccaggtgg ccataatccc
     1021 ccgcccgcgt gccttgtaag cgccggctgt atcaatccca acataatgca cccgaaacgg
     1081 gcgctcgcga gccggaatcc gttgctcact gatcgagcga tctccggtcg caaacgaaat
     1141 tggcacttcg ccaagcgcat ccttccagac cagttgatgg cgatacctct tcgtgctggc
     1201 ctccggcatg acaccataga aatacgcatt aaccgattgg gcatggtcat cgggcgtgag
     1261 tgcttcagcg cacttggcgt tcaatcctct gatctccgcc gccagcacgt cgttctcata
     1321 aatattcagc gtgaaatctc caaaggcatc tctccccgac atatagttaa ctgcccggtt
     1381 gtcgattgcg aacttggcta cataataagc cggcgattga ctgtggagct ggtgcgggta
     1441 aacgtcatcc accagctggg tcagagcact gctgatcgtc atgcgcggcg caaccgtcac
     1501 ctcctccgtg cgagtgtcgg ttccgcctcc attcacctca acttccagct cccccgcgct
     1561 gtcgctatac tcaaaggcac ttacgattcc ttgcagctcc agccgggctc cggacacggg
     1621 tcgccccagc ggatctttca cctggatctt caacttccct ttgaatccat acaagtcttc
     1681 tatccgcatt cgacattcgc catacccgac tccatctcct acgacagtca gcgcggcaaa
     1741 gggcggcgcc acgtaggcat cacgcacggt ctcgataacc gccccgccac tgccaacggc
     1801 aatctcgacc tccccctgct tgtcggtctc gaaccacggt gtcagatagt acgtcacctc
     1861 ttcatacgaa ggacgcaaca ccgccttgca gcgcgccctg aactgcaccc ccggcgccgg
     1921 catcgtagcg ttctcattaa acggatcgta agttgtcgcc ttggtccgga aggtcaagtt
     1981 cagcaaatcc ggagagtccg tcagttcgtt cagattgtag gttctcccct cgaaacaatc
     2041 gttaaacgtt tggatcggtt gtccttctgc cattcggtat tccagaaata caacagcacg
     2101 gctgatattc attgcattgc tgatcgtgac acttcctgaa ttgctaccgc tggcggcaaa
     2161 taccgagctg aagtacgtgc ccagctcgct gacttttgca tcaagctcaa cagccgtcgt
     2221 ccacgaaatg aaatccgcgc tcatcaccgc ctgggtgatc tccgcatcac ggggcagcac
     2281 gtcaggatag ctatgctgca gcgtcaccgg atgctctcca tgccccacaa atagtgcgtc
     2341 gccccaggcg agcagggacg gcccccggct ctcaaccgcg agcttcacgg tgactgaatc
     2401 gaccccgttc gaaaacgcct tcgtcgtgct gacctccaca cggtcccgct ccgtccatcc
     2461 cggttgcagc ccggtctgat cctgggccca tagcggttgc agtaccaagc caatcaacac
     2521 acatgacagc gcaacgcgaa atcgagacat gatcccattt tccgtttcta tccggttcac
     2581 accgttgttc caagcagtcc gccttttaga tctcaaactc tgctgtatac cctaccactt
     2641 tccccaggga aatccagtag aatcatgggg cagcacgatc cgaatcccgg ttcaccgcgt
     2701 catgcaatca tcagcacctt gacacttcac attcgcgcaa acacgcaaag gctccgtcca
     2761 cgccgaattc cctcactcag cggcgccacc tgcttggctg acgtgctgct ggcgcgtgag
     2821 ctgtcatcca cttcttttct ctatccactt ctcttccgct gggctaagtt aagagcgaag
     2881 gttttgcgaa gaggcgcgcc ccttccgtgc cgatacttca ccgg
//
LOCUS       DAOPBQ010000484         2921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26861_length_2921_cov_4.054082, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000484 DAOPBQ010000000
VERSION     DAOPBQ010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26861_length_2921_cov_4.054082"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKW60_13170"
     CDS             complement(<1..514)
                     /locus_tag="PKW60_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00431.1"
                     /translation="MITPLPTAYRIIGQNASSKLHILSILHDYMPLISGKSADPPHFP
                     RNLAFGTQLVSLPVMKVAIPEWQGRISPVFDVAATLVVSNFAADGQAIEQHSIQLFRN
                     DLHGRVMEMTGLGIDVLICGAISWPLEMALSNAGIEVIPHTCGEVQSVLESYKANRLQ
                     PNTFLMPGCCG"
     gene            513..1877
                     /locus_tag="PKW60_13175"
     CDS             513..1877
                     /locus_tag="PKW60_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPK00432.1"
                     /translation="MNPTQQAAQREAAILDSVNEGVFAVDQDWRITCFNSAAERITGV
                     LRDQAIGNRCCDVFRASICETDCALRKTMSEGRPVVNATAHIVNHEGQRIPIRISTAL
                     LRDESGAVIGGVESFQDLTQIEQLQKELENRYTFEDIVGRSPAMQRLFDILPQVSASA
                     STVLIEGASGTGKELFARAIHNLSPRRKKPFVAINCAALPDTLLESELFGHKAGAFTD
                     AKRDKPGRFELAQGGTIFLDEIGDISPAMQVRLLRVLQERQIEPLGGLKPLEVDVRVV
                     AATNKDLAELVQSGQFRDDLYYRIRVVYLKLPELKQRREDIPLLVNSLIAKFNRLQGK
                     DIAGVSGKVMARLMEYDFPGNVRELENIIEQAFVLCRGGLIEGKHMPPELRSATGVER
                     DEPRAMSLDAMERFLIREALARHSGNRKKAAAELGVDVSTLYRKIRTLGIAVPERDGR
                     GQRR"
     gene            2091..2453
                     /locus_tag="PKW60_13180"
     CDS             2091..2453
                     /locus_tag="PKW60_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPK00433.1"
                     /translation="MKIAVTAQGTDLNAALDPRFGRAKFFVVVDTEAEGFEAVDNSMN
                     LNAAQGAGIQAGRNVIELGVEAVITGHVGPKAYTTLQSGGVGVYTGASGTVGEAVEQF
                     KAGALKRQDGADVEGHWV"
     gene            2684..>2921
                     /locus_tag="PKW60_13185"
     CDS             2684..>2921
                     /locus_tag="PKW60_13185"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPK00434.1"
                     /translation="MNAGVKSGITVLDFGCGPGGFTLAAAKLAGPEGRVLAFDIHPLA
                     VKFVEKGAARRGLGNVQTFLKSSVAEVPEESVDMV"
BASE COUNT          634 a          787 c          939 g          561 t
ORIGIN      
        1 tgccgcagca accgggcatc aaaaacgtgt tcggctggag tcggttcgcc ttgtacgatt
       61 ccagaacgga ttgcacctcg ccacaggtgt gagggattac ctcgataccc gcgtttgata
      121 aagccatttc gagaggccag gagatggcgc cgcagatcag cacgtcgatg cctaaccccg
      181 tcatttccat cacgcggccg tgcagatcgt ttcgaaacag ctgaatcgag tgctgttcaa
      241 tggcctggcc gtctgcagca aagttgctca ccaccaaggt ggcggcaacg tcgaataccg
      301 gggaaatccg gccctgccat tctggaattg ctactttcat gaccggtaaa gacacaagtt
      361 gtgtgccaaa cgccaaattt cgcggaaaat gggggggatc agcgcttttt ccagaaataa
      421 gaggcatata atcgtgcaaa atagacaaaa tatggagttt agagctggcg ttttgcccga
      481 ttatgcggta tgctgtcggt aaaggagtga tcatgaaccc aacacagcag gccgcgcagc
      541 gggaagccgc tatcctcgac tcggtgaatg agggcgtatt tgcggtcgat caagactggc
      601 ggatcacgtg tttcaacagc gcggctgagc gcatcacggg cgttttacgg gaccaggcca
      661 tcgggaaccg gtgttgcgat gtgtttcgcg ccagcatctg cgagacggat tgcgcgctgc
      721 ggaaaacgat gtctgagggg aggccggtgg tgaatgcgac cgcacacatc gtgaatcacg
      781 agggccagcg catccccatt cgcatctcga ccgcattgct ccgcgacgag tccggtgcag
      841 tgatcggggg cgtggaatcc tttcaggatc tgacgcagat tgagcaactg cagaaagagc
      901 tggagaaccg ctacacattt gaggacattg tggggcggag ccccgcgatg cagcggctgt
      961 ttgatatcct gccgcaggtt tctgccagcg cgagcacggt cttgatcgaa ggcgcgagtg
     1021 gcaccgggaa agaactgttc gcgcgcgcga ttcataatct ctcgcccagg cgcaagaagc
     1081 cctttgtggc catcaactgc gccgctctgc cggatacgct gctcgaaagc gaactgttcg
     1141 gccataaggc cggcgcgttt acggatgcca agcgagacaa gcccgggcgc tttgagctgg
     1201 cccagggcgg caccatcttt ctggacgaga tcggcgatat ctctcccgcg atgcaggtgc
     1261 gcctgttgcg ggtgctgcag gagcgccaga tcgagccgct gggcggcctc aaaccgttgg
     1321 aagtcgatgt tcgggtcgtg gccgcgacca acaaggatct tgcggaactg gtgcaatccg
     1381 gccagttccg tgacgacctc tattaccgga ttcgtgtggt gtatctgaag cttcccgagc
     1441 tcaagcagcg ccgggaagac atcccgctgc tggtcaacag cctcatcgca aaattcaacc
     1501 ggctgcaggg taaagacatc gcgggggtat cgggtaaggt gatggcccgg ctcatggaat
     1561 acgactttcc gggcaacgtt cgcgagctgg agaacatcat cgagcaggcc tttgtcctgt
     1621 gccgcggcgg cctgatcgag ggcaaacaca tgccccccga actgcgctcc gccaccggcg
     1681 tggaacgcga cgaaccgcgg gcaatgagcc ttgacgccat ggaacggttt ttgattcgag
     1741 aggcactcgc ccgtcattcg ggaaaccgta agaaagcggc cgccgagttg ggcgtagacg
     1801 tcagtacgct ctatcgaaag atccgcaccc ttggaatcgc cgttccggag agagacggcc
     1861 gcggccagcg ccgataatcg tgcacaatgc atgattgagg ccgttggcgc cgggcttcgt
     1921 tcaagggaat ccccgggaaa ctggagacac acacccggcg ctgttcttct cgcagtcgcg
     1981 tgcatacgcc cgtaatagag gggattttga ggagaccgcc ggtgtgcaaa ggcgccggct
     2041 gcccggcttg gcacatagct tgctgttatc gctggcatgg aggaacaatg atgaaaattg
     2101 cagtgacagc gcagggtact gatttgaatg ccgctctgga tccccggttt ggccgggcga
     2161 agttttttgt tgttgtagat accgaggcgg aaggcttcga ggcagtcgat aacagcatga
     2221 acctcaatgc ggcccagggc gcgggcatcc aggccggcag aaacgtaatc gaactgggtg
     2281 tggaagccgt gatcacgggt cacgtggggc ccaaggccta tacgacgctc cagtcgggcg
     2341 gcgttggtgt gtatacgggc gcgtcaggca cggtggggga ggccgtcgaa caatttaagg
     2401 cgggcgcact caaacgccag gacggtgcgg atgttgaggg gcactgggtc tgatgaacgc
     2461 gctgccgtga agaagcaccc gcccaacaag tgcgctggct gtgaaacgca tagccggcca
     2521 gagcaggcac cgctgttcac ggggcattga tgggaacagg ataatccacc accggggctg
     2581 ataacgcatg agcaagccgc atacgcagag caacgctgca ttccgcatga tgtctttgaa
     2641 attccgcatg cgggactggg caaaccctcc caggaaaatt ctgatgaacg ccggggtgaa
     2701 atccggcata acggttttgg atttcggctg tgggccgggc ggttttacat tggcagccgc
     2761 caaattagcc gggccggaag ggcgcgtcct ggccttcgac atccacccgc ttgcagtgaa
     2821 gtttgtcgag aaaggggcag ctcggcgggg cttgggcaac gtgcagacgt ttcttaaaag
     2881 cagcgtggcg gaggtcccgg aagagagcgt ggatatggtc c
//
LOCUS       DAOPBQ010000485         2913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26957_length_2913_cov_3.834500, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000485 DAOPBQ010000000
VERSION     DAOPBQ010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26957_length_2913_cov_3.834500"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="PKW60_13190"
     CDS             complement(<1..1198)
                     /locus_tag="PKW60_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HPK00435.1"
                     /translation="MGVKAMQEHKHHSGCCGCSLGPIERRSFLTALGATAGLAAAAGC
                     ATTATAPLVAAGPRPKPCLEKKPLIVQPALAYHIYSQRDQTSWRPWGGLHTEGDIDAE
                     INRIEHELRALSKQAEFPVSFKPLARVRDENEAAALCAQPADVMLIYGANSGMETIEK
                     LITTDRYNLVFVRHKSGPVYLWYEIVHPRLLRKNVDEYGQPGLEPSDVVVDEYGDLLW
                     RLRSLYALHNTVGSRIVCIGGPSGWSEGGEKAPEIAANLWQMDLVTVEYDDVGARIKA
                     ARANPERVKRAEAEAAAYLKTPGTHLKTDPGFVSRAFLLTEVFEELMAEAGAQAITVN
                     HCMGTIMPLSETTACLPLSLINDAGALAFCESDFAVIPSGVLLHHIASMPVFLQDPTY
                     PHHGLIT"
     gene            2069..2614
                     /locus_tag="PKW60_13195"
     CDS             2069..2614
                     /locus_tag="PKW60_13195"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPK00436.1"
                     /translation="MSSKGDGSVRILGEGRFLRLVDRDTWEHVERVRVSGVVCMVAVT
                     PDNALLLVEQYRPALRCATIEIPAGLVGDLPGTENEAFETAATRELEEETGYTAARMR
                     FLSEGPTSSGICSETIRFYRAEGLRKIGDGGGDHSEDITVHVVPLQGIHDWLETQTER
                     GVLIDPKVYAGLYFVGQGAPH"
     gene            2703..>2913
                     /locus_tag="PKW60_13200"
     CDS             2703..>2913
                     /locus_tag="PKW60_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00437.1"
                     /translation="MLKRAGFCCALVMVAALGAEAQEATYTAALLTPASEAWLPVRDL
                     LEEHLRSAGYHVVPFGFDELSDPAKL"
BASE COUNT          564 a          842 c          915 g          592 t
ORIGIN      
        1 gggtgatcag gccgtggtgc gggtaggtcg gatcctgcag gaacacgggc atcgaggcga
       61 tgtggtggag caggacgccc gacgggatca ccgcgaaatc ggattcgcag aaggccagcg
      121 cgcccgcgtc gttgatgagg ctcaggggga ggcaggcggt ggtttcggac agcggcatga
      181 tcgtgcccat gcagtggttc acggtgatgg cctgggcgcc ggcctcggcc ataagctcct
      241 caaacacctc cgtcaacaga aacgcgcggc tgacaaagcc cgggtcggtt ttcaggtgcg
      301 tgccgggggt cttgaggtaa gcggccgctt cggcctcagc ccgcttcacg cgttcaggat
      361 tcgcgcgggc tgccttgatg cgcgcgccca catcgtcgta ttcgaccgtg acgaggtcca
      421 tctgccagag atttgctgcg atttcagggg ctttctcgcc accctcgctc cagccgctcg
      481 ggccgccgat gcagacgatg cgagagccga cggtgttgtg gagcgcataa agagagcgca
      541 accgccagag caggtcgccg tattcatcaa caacgacatc gctgggctcg agcccgggct
      601 ggccatactc gtcgacgttc ttgcgcaaca ggcggggatg gacgatctcg taccagaggt
      661 agacgggccc ggatttgtgc cgcacgaaca ccaggttgta ccggtcggtc gtgatgagtt
      721 tttcgatggt ttccatgccc gaatttgcgc catagatgag catgacgtcg gcgggctggg
      781 cgcacagcgc ggccgcttca ttctcgtcgc gcacgcgggc cagcggtttg aaggacacgg
      841 gaaattcggc ctgcttcgac agggcgcgga gttcgtgctc gatgcgattg atttcggcgt
      901 cgatgtcccc ttccgtgtgt aatccacccc agggccgcca gctggtctgg tcgcgctgcg
      961 agtaaatgtg gtacgcgagt gcgggttgaa cgatcagggg tttcttttca aggcagggtt
     1021 tggggcgcgg cccggcagcc acgagcggcg ccgtggcggt cgtcgcgcac cccgctgcgg
     1081 ccgcgagacc ggccgtcgcc cccagggcgg tgagaaacga ccgccgttca atgggcccca
     1141 gagaacaacc gcaacagccc gaatggtgtt tatgctcctg catggccttc acccccatgt
     1201 gcgccttcgg cgcttactcg gctgatccat cctccattga cgcgcccagt gtaccacaca
     1261 ggcggccatc cagggtgtga tgctgcaggg aaaagatcag ggctccagaa acagatgcgg
     1321 aggtgcaatc cggtacctcc tggtcttgcc tttcttcgcg caccaaggcc cgggcccacc
     1381 caaattgctc ttgcgagatc aagtcggcaa cggttgtgag tctgcctcta cgcgccaacc
     1441 tgcctgtctt cgtgtcaaaa agcggaagaa ctacctcaac atccttcttg atttgttcaa
     1501 tcgctccacc tgcaagccga aaagtccggt gtggcggctc gaccttccaa aagacgtctg
     1561 agccgtccgg catcagtgtc catgccatgc aggcgactcc gagttccgcg cgatcaacgt
     1621 cccacgtgca ggggcctcgc catgcgtctt ccgcttctga gtactcaaag aactcccaag
     1681 cgtttgtatc cagcgaaaaa ctgagacaat tcgtgccgac gcggagatgc tgagtgaaat
     1741 gtaagactct gcatcttcgt actcgacacg ctggcgttcc tccagtgata tggccccggg
     1801 ctgctgtctg ccgtttgagg gtggcatgat atcgcccgaa gcggaaagct ccgaacttaa
     1861 aagaaaccac aatagaaaca gttgcatact cttccgtgtt ctcatttatc tatttctcat
     1921 tctatatgct gtgtttcggc ttcggcaagg gcacaatgcc accgtggctc cacggcctga
     1981 acacgcagcg gacccggccg gtagcacaag agcctgcaag acgttatact ttgagatcct
     2041 gcggaaatta catcaaaagg aactcactat gtcttcgaag ggcgatggtt cggttcggat
     2101 cctgggtgaa ggccgctttc tccggctggt ggatcgtgat acgtgggagc atgtcgagcg
     2161 tgtccgggtg tctggcgtgg tgtgcatggt ggctgttacg ccggataacg ccctgcttct
     2221 cgtggagcaa taccgcccgg cgctgaggtg cgccacgatt gagatcccgg ccgggctcgt
     2281 gggcgatctg cccggcacag aaaacgaggc gtttgagact gccgccacgc gcgaacttga
     2341 agaagaaacc ggctacacgg cggcccgcat gcggtttttg agcgaaggac cgacgtcgtc
     2401 gggcatctgc tcggagacca tccggtttta ccgggctgag ggcctgcgga agatcggcga
     2461 tggcggcggg gaccattccg aggacatcac ggtgcatgtg gtgcctctcc agggcattca
     2521 tgactggctg gagactcaga ccgaacgcgg cgtgctcatt gatccgaagg tctatgccgg
     2581 gctgtatttc gttgggcagg gtgcaccgca ctgaacaaaa aagagagctt ggatcatcgc
     2641 gataacagat tttcccgaat ctctatcgaa gaacctggag caattacgga aagggagaga
     2701 atatgctgaa acgggccggg ttttgttgcg cattggtgat ggtcgccgct ctgggcgcgg
     2761 aagctcaaga ggcaacctat acggccgctt tgctgacacc cgcatccgaa gcctggctgc
     2821 cggtgcgcga cctgctcgag gagcatctca ggagcgcggg ctatcacgtc gtgccttttg
     2881 gcttcgacga gctttccgat ccggcaaaac tca
//
LOCUS       DAOPBQ010000486         2911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_26988_length_2911_cov_3.068277, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000486 DAOPBQ010000000
VERSION     DAOPBQ010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26988_length_2911_cov_3.068277"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PKW60_13205"
     CDS             complement(<1..632)
                     /locus_tag="PKW60_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPK00438.1"
                     /translation="MRQPALLPKSRLLVAVNRNAEEELACAEEYGLGVEIQTFSLPEI
                     LAKDASALLEKVAERVRRLPGPIGVHGPYIDTAHASSDLEIVRVCRNRYLAALDTARA
                     FDARYVLFHSQYNTMLKLEAYPDIYHEASLRFWPEILEKAASWGLNIYIENMFDVDPA
                     PLRRLADAFDTPAFKLCLDLAHSQIYSQRSFEEWVTAFGRHLAHVHMNDTR"
     gene            complement(634..1767)
                     /gene="mltG"
                     /locus_tag="PKW60_13210"
     CDS             complement(634..1767)
                     /gene="mltG"
                     /locus_tag="PKW60_13210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPK00439.1"
                     /translation="MSTEPDTQQSRRAWSCLRRAAKLSLLLLVLLLLGIAAVGVGAYL
                     AYEHVVRPGSPGDTVAITIPEGLSGKAVGQLLESQGLVEHELFFRLAMRLDKTGRPIK
                     HGYYRLPLGLSPAQLLTHLQEGTQWRMSPEEIPDDLKVTVPEGLTIVQMSAQFDDPEA
                     FQEAAADPQLIARLGLEVPNLEGFLMPNTYYFEKKPDERAVVERLLNQFMREYAELCK
                     DIPEAAGRDLLEVVTVASLIEEECRVDEERPLVAAVIYNRIRAGMPLDLDSTLQYALG
                     KYGARMLNEDKEADSPYNTYRRAGLPPGPISNPGVACLRAALEPAAVDYLFFVSNADG
                     KTHTFSSTMNEHAEAVRRFRKEIAVQRRALREQETRREGHGAH"
     gene            complement(1776..2210)
                     /gene="ruvX"
                     /locus_tag="PKW60_13215"
     CDS             complement(1776..2210)
                     /gene="ruvX"
                     /locus_tag="PKW60_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPK00440.1"
                     /translation="MPEEGRIIGLDIGDVRIGIAVSDPLGITAQGREVLSARSPEEDA
                     AAICALAKEVEAIRVVVGLPLDQNGEIGPQAAKVLAFVETLRELLDIDIVTQDERFST
                     AAAQRALIDANMRRKKRKSVIDMVAAQHILQTYLDRQRRPGI"
     gene            complement(2284..2709)
                     /locus_tag="PKW60_13220"
     CDS             complement(2284..2709)
                     /locus_tag="PKW60_13220"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPK00441.1"
                     /translation="MEENRRLNREVTRWKQAAATGGAVDYMSRVQDVDGIKLLAVEVA
                     NQDADGLRHVMDKLRDQLGSGVVVLASAAEDKAVLCVGVTKDLTSRAKAGDLVKRLAS
                     IVGGGGGGRADMAQAGGKHPEKVGEAIAQAPEILRQCLS"
     gene            complement(2716..>2911)
                     /locus_tag="PKW60_13225"
     CDS             complement(2716..>2911)
                     /locus_tag="PKW60_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00442.1"
                     /translation="THVARTGVIGAFKILSESSIAAGVRRIEAVCAERALETIQTQEE
                     QLSRVAQMLNASTDTVLARV"
BASE COUNT          526 a          910 c          848 g          627 t
ORIGIN      
        1 cgtgtatcgt tcatatgcac gtgagccagg tggcggccga aggccgtgac ccattcctca
       61 aagcttcgct ggctgtagat ctgactgtgg gcgaggtcga ggcatagctt aaatgccggt
      121 gtgtcaaagg catccgccag acggcgcaac ggcgcgggat cgacgtcaaa catgttttcg
      181 atgtaaatat tcagtcccca tgacgccgcc ttttccagta tctccggcca gaagcgcagg
      241 ctcgcttcgt gatatatatc gggataggct tccagtttca acatcgtgtt gtactgactg
      301 tgaaacagca cgtagcgcgc gtcaaaggcc cgggcggtgt cgagcgcggc aagataccgg
      361 ttccggcaaa cgcggacgat ttccagatcg gagctggcgt gggccgtatc aatatagggc
      421 ccgtggacgc caattgggcc gggtaagcgc cgcacccgtt ccgcgacctt ttccaagagc
      481 gctgaggcgt ctttcgcgag tatttcagga agggaaaagg tctggatttc gacccccagg
      541 ccgtattctt ccgcgcaggc cagttcttcc tctgcattgc ggtttacggc caccaggagc
      601 cggcttttgg ggagtaatgc aggttgtcgc atctcagtgg gcgccatgtc cttctctacg
      661 agtctcttgc tcacgcaacg cgcggcgttg caccgcgatc tcttttcgaa aacgccgcac
      721 agcctcggca tgttcattca tggtagagct gaatgtgtgc gtctttccgt cggcgttgga
      781 cacgaaaaag aggtaatcca cagcagcagg ttccagggcc gcccgcaggc aggctacccc
      841 cgggtttgat atgggtcccg gtggtaagcc agcgcgccgg tatgtgttgt acggcgaatc
      901 cgcttctttg tcttcattaa gcatccgagc accgtatttc cccaaagcat actgcagcgt
      961 gctgtccaga tccagcggca tgcctgccct gatccggttg tagatcaccg cagccaccag
     1021 cgggcgctct tcgtcaaccc ggcattcctc ctcaatcagc gaggctaccg tgaccacttc
     1081 aagaaggtca cggcccgcag cctccggtat atccttacat aattcggcat attcgcgcat
     1141 aaactgattc agcaggcgtt ccaccacggc gcgttcgtcg ggttttttct caaaatagta
     1201 ggtgttcggc atcagaaagc cctccagatt gggcacttcg agccccagcc gcgcgatgag
     1261 ctgaggatcc gcggcagctt cctggaacgc ttcaggatcg tcgaactggg cggacatctg
     1321 cacaatggtg agtccttctg ggaccgtgac cttgaggtcg tcggggatct cctccggcga
     1381 catgcgccat tgggtacctt cctgcagatg ggtcagaagc tgtgccggcg acaaccccaa
     1441 tgggagccgg tagtagccgt gtttaatcgg gcggccggtc ttatccagtc tcattgcgag
     1501 ccgaaaaaaa agctcgtgtt ccacaagacc ttgactttca agcagttgcc cgaccgcttt
     1561 gccgctaagg ccttccggga tcgtgatggc gacggtatcc ccaggcgaac cggggcgaac
     1621 tacatgctca taggccaggt acgcccctac gcccacggcc gcaatcccca gaagcaacag
     1681 caccaggagc aggaggctca gctttgctgc tcgacgcagg cacgaccacg cccgccggga
     1741 ctgctgcgta tcgggctctg tgctcatcgc aatgcttaga ttcccgggcg gcgctgccgg
     1801 tcaagatagg tctgcagtat gtgttgcgcc gccaccatgt cgattacgct tttgcgcttc
     1861 tttcttcgca tattcgcgtc gatcagggct cgttgagccg cggccgttga gaaacgctcg
     1921 tcctgggtta cgatatcaat atccaggagt tcgcgcagag tttccacaaa cgccagtact
     1981 ttggctgcct gggggccaat ctcgccgttc tggtccagag gcagccccac gacgacccgt
     2041 atggcctcca cttctttggc aagcgcacag atcgcggccg cgtcttcttc gggagagcgt
     2101 gcggaaagca cctcgcggcc ctgcgccgtg attcccagag gatcgctcac cgcgatccct
     2161 atccgcacgt cgccgatatc cagtccgatg atccggccct cttcaggcat tggcatctcc
     2221 tcacagtccg tcgtcgatgc attccgcgcc cgccgtgcgg gtgctcgcag cgcccccccg
     2281 tgtttacgac agacactggc gcaggatctc cggcgcctgg gcaatagctt cgccgacctt
     2341 ctccgggtgc ttgcctccag cctgggccat gtccgcgcgt ccgccaccgc ccccgcccac
     2401 aatggacgcg aggcgcttta ccaggtcgcc ggccttcgcc cgcgacgtca agtctttggt
     2461 gactccgacg cacaaaacgg ctttgtcttc ggcagcggag gccagcacga cgactccgga
     2521 cccgagttga tcgcgcaatt tgtccatcac atggcgcaat ccgtcggcgt cctggtttgc
     2581 gacttcgacg gccaggagtt ttatgccatc gacgtcctgg acccgggaca tataatcaac
     2641 ggctccgccg gtggccgcgg cctgtttcca gcgggtgact tcgcggttca gacggcggtt
     2701 ttcctccagg actgcctaca cgcgtgcgag cacggtgtcg gtgctggcgt tcagcatctg
     2761 ggctacgcgc gagagttgtt cttcttgcgt ctggatggtt tccagggcgc gttcagcgca
     2821 taccgcctcg attcgccgca cgccggcggc aatggagctc tcgctcagga tcttgaacgc
     2881 gccgatcact ccggtgcggg caacgtgggt g
//
LOCUS       DAOPBQ010000487         2879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_27354_length_2879_cov_2.905453, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000487 DAOPBQ010000000
VERSION     DAOPBQ010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27354_length_2879_cov_2.905453"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..2609
                     /locus_tag="PKW60_13230"
     CDS             162..2609
                     /locus_tag="PKW60_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00443.1"
                     /translation="MQERLACFAMVLGAVLIAAVPCMALDLSGAVIVPLPADGVVNKA
                     AEMLQEELAARSGITLSIAAETPKTGAAILLGTVQSIPGVSVPDKPEAYGIALDGNTV
                     KLVGHDVRGALFAAGRLIRLAKAEPGALSLELARPIATAPNAAYRAHQLGYRNTANTY
                     DAWSIETYEQYIRDLILFGCNGIEIIPNLDPEAKDGPVMAMPMRAMNKQLSALLQAYG
                     IDLWLWSPVMAEPGEDVTTPEGKAEALEKRRQMFKDYPSIAHVFVPGGDDGRTPARDL
                     MPFLEDLAPILHEYHPNAKIWVSNQTFTLDENDYFFDYLRDADPQWLHGLVYGPWVKM
                     AWEEMRERTPKRFPIRDYPDITHTLRCQYPVPNWDPAFANTIGREPMMPMPEMQRHIY
                     LRYRNVSDGFGTYSDGVHDDMNKMLWSTLGWDPEADLDTFLEEYGKVFFSGALAGEVA
                     RGLRMLEQNWHGPILENQNIPATRDLWERIAPRVPDFDKHWRAQMYLFRARFDANVQE
                     ETRAQRGYEKEAHAALALARTKGVDAAIEQAEIALAKADTPANPELRSSIEALGPLMW
                     NSIGYQLSVEPPYLAKNTERGAMLDWLDQPLNDRPWLEQRFEAIRVLDSKEAQLEAID
                     QILNWTDPGPGGFYDNLGAVGEFSHVVYQRPWEEDPSACRTARVAFMKYKADQAAIKA
                     EASQAEADNMVFKEEVARLAGAFRGRQELRMSWQSQMTTLYGTPLKMRYEGLDPEAQY
                     KLRVTYVGRFKPSMVLTLNDEYCVHGPVPQPSPVWPLEYHLPRSQTSSGTLDIEWNLV
                     DGRGCMVAEVWLIKVPE"
     gene            2793..2869
                     /locus_tag="PKW60_13235"
     tRNA            2793..2869
                     /locus_tag="PKW60_13235"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2827..2829,aa:Val,seq:tac)
BASE COUNT          565 a          866 c          928 g          520 t
ORIGIN      
        1 ctgcgggatt tgcgggatca agtggcttcg agaaaaccgc gcctggctgc gcggccggtg
       61 ggcgcggtgt gcggtttggg ggaggggcgg gcaatttagt atgatgtgtc cgaaatacgg
      121 ggggctgtgg ttttccaaga atggccgaga ggagaggtgt gatgcaggag cgtctggctt
      181 gttttgctat ggtcttgggt gcggtgttga tcgcggcggt gccgtgcatg gcgctggatt
      241 tgtccggggc ggttattgtg ccgctgcccg ccgatggggt ggtcaacaag gcggcggaga
      301 tgttgcagga ggagctggcg gcgcgcagtg gcataaccct gtccatcgcg gctgagacgc
      361 caaagacggg cgccgcaatt ttgctgggca ccgtgcagtc gatcccgggc gtttccgttc
      421 cggacaagcc ggaggcgtat gggatcgcgt tggatggaaa caccgtgaaa ctcgtggggc
      481 acgacgtgcg cggggccctg tttgccgccg gacgcctgat ccgcctggcc aaggcagagc
      541 ccggggcact ctctctggag ctggcccgtc ccatagcgac cgcgccgaat gcggcctacc
      601 gggcgcacca gctgggatat cgcaacaccg cgaatacata cgatgcctgg agcattgaga
      661 cgtacgaaca atacatccgg gacctgatcc tgttcggctg taacgggatt gagatcatcc
      721 cgaacctcga cccggaggcc aaagacggcc ccgtaatggc tatgccgatg cgcgcgatga
      781 acaagcagtt gtcggcgttg ctccaggcct acggcatcga tctgtggctc tggtcccccg
      841 tgatggctga gccgggagaa gacgtgacca cgccggaagg caaggcggag gcccttgaga
      901 aacgccgcca gatgtttaag gattatccgt cgatcgcgca tgtgtttgtg cccggcggcg
      961 acgacggccg gacccctgcc agggacctga tgccgtttct ggaagatctg gcgcccattc
     1021 tccacgaata ccatcccaat gcgaagatct gggtctcaaa ccagaccttc accctcgacg
     1081 aaaacgatta ttttttcgac tacctgcgcg acgcagatcc ccaatggctg cacggcctcg
     1141 tctatgggcc ctgggtcaag atggcgtggg aggaaatgcg cgaacggacc ccgaaacgct
     1201 ttcccatccg cgactatccc gatatcaccc atacccttcg ctgccagtat cccgtgccga
     1261 actgggatcc cgcgttcgcc aacaccatcg gccgggaacc catgatgccc atgccggaga
     1321 tgcagcggca tatctacctg cgttaccgca atgtcagtga cgggttcggc acgtattcgg
     1381 acggcgtaca cgacgacatg aacaagatgc tctggagcac cctgggctgg gatccggagg
     1441 ctgatctcga caccttcctc gaagagtatg gcaaggtgtt tttcagcggt gcgctggccg
     1501 gcgaggtcgc ccggggcctg cgcatgctgg aacagaactg gcacggtccc attctggaaa
     1561 accagaatat tcccgcgacg cgcgacctct gggagcgtat tgcgccgcgc gtgcccgatt
     1621 ttgacaagca ctggcgcgcc cagatgtact tgttccgggc tcgttttgat gccaacgtgc
     1681 aagaagagac ccgcgcccag cgcggttatg aaaaggaagc gcatgccgcc ctggcgctgg
     1741 cgcgcaccaa aggcgtcgac gccgccattg aacaggccga gatcgcgctg gccaaggccg
     1801 acaccccggc gaaccccgag ctgcgcagca gcatcgaggc gctgggcccc ctcatgtgga
     1861 acagcatcgg gtatcaactg agcgtcgagc cgccttatct ggccaaaaat accgaacgcg
     1921 gggccatgct cgactggctt gaccagcccc tcaacgaccg gccctggctg gaacagcgct
     1981 ttgaggcgat ccgggtgctg gattcaaaag aggcgcaact cgaagccatc gaccagatcc
     2041 tgaattggac cgatcccggc cccggcggtt tctacgacaa cctgggtgcg gtaggcgaat
     2101 tttcgcatgt ggtctaccag cgcccctggg aggaggatcc ctccgcctgc cggacggctc
     2161 gcgtagcctt catgaagtac aaggccgacc aggccgccat caaagcggaa gcaagccagg
     2221 ccgaagccga caacatggtg tttaaggaag aagtggcgcg tctggccggg gctttccggg
     2281 gccggcagga actgcgcatg tcctggcagt cgcaaatgac aaccctctac ggcaccccgc
     2341 tcaaaatgcg ctacgaaggg ctggatcccg aagcgcagta caaactccgc gtgacctatg
     2401 tcggccgttt caaaccctcc atggtgctca cgctcaacga cgaatactgc gtccatggcc
     2461 ccgtaccgca gcccagcccg gtctggccgt tggaatacca tctgccgcgc agtcagacca
     2521 gctccggtac gctcgacatc gaatggaacc tggttgatgg gcgcggctgc atggttgcgg
     2581 aggtgtggct gatcaaagtc ccggaataat ccaggcgcac tgacgctgcg cggcggctct
     2641 gcgccgagag ttgccgttat tcgcaggaaa taaatcgacg gacccggcag cggggccggt
     2701 gaccatccgg cggcgaggac gacggggcac aggagcccgg gcacggtttg ccccgcgcct
     2761 cctccgatat ggtatacttg cgctgcacgg gggggcggtt agctcagtag gttagagcac
     2821 ctgctttaca cgcagggggt cattggttcg agtccagtac cgcccaccat atggttcac
//
LOCUS       DAOPBQ010000488         2878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_27367_length_2878_cov_2.658165, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000488 DAOPBQ010000000
VERSION     DAOPBQ010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27367_length_2878_cov_2.658165"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PKW60_13240"
     CDS             complement(<1..575)
                     /locus_tag="PKW60_13240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPK00444.1"
                     /translation="MKRIHPILQEYWGFESFRPFQHEAVEAILEGRDSLVVLPTGGGK
                     SLCYQAPALALEGLAVVVSPLISLMKDQVDALRQRGVPAHCLNSAQSPEEQRNVFADL
                     RSGRTRLLYAAPERLTQTYFLEFLRTQRVAYFVVDEAHCISMWGHDFRPSYRALSCLR
                     EQFPQARIHAYTATATQRVRDDIIRSLRLEAL"
     gene            complement(649..1806)
                     /locus_tag="PKW60_13245"
     CDS             complement(649..1806)
                     /locus_tag="PKW60_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK00445.1"
                     /translation="MTSRDRILAAIAHREPDRVPLDLAGTHVTGISRKAYEAVCAYLG
                     LSDETPEWQDVVQQTVIPSDAFLDRFGVDTRGLFPLTSHNWDVFGQLEDGGAVWVYRD
                     EWGMVQHFPKENGLWFSIVKHPLAELPPEPSTIAAYPWPSAADPQRIHGLRDHAARFR
                     GQGRAVMLKGLCAGIFEMAQRLRGMELALMDPLVYPAFADCLYGRLADLKIAFWEMAL
                     RALGPEVDIVVENDDYGTQQSQLISFEQYTSTIRPHLERVLRSIKRCAPHVRLFLHSC
                     GNVRPYLPGFIEAGVDIINPVHVTAAGMEPRALKRDFGDAITFWGGGVDTQHILPHGT
                     PQEVKDDVKRNVDALAPGGGYVFSSIHNIQSDVPPENIVAMYEALAECGRY"
     gene            2179..>2878
                     /locus_tag="PKW60_13250"
     CDS             2179..>2878
                     /locus_tag="PKW60_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00446.1"
                     /translation="MKTSACLLVLASIIILVIPAWAKPNEVPLPLGKRLIEYGWDVPT
                     TEQVRKNINKMERRPFDGLVFRLKGGGNVFVPEPWGESRFKEDYDNAERIKWERFTDN
                     FVIMWAASDQDWFNDSQWEQIIINTRLVAHAARLARCVGICFDAEPYGENPWEYAKTA
                     HHDTYSFAEYQAKVRQRGAQFIQAIEAELPDPQILTFFQLSYFANLCVPMDPGFRQEK
                     LSKEYYALLPAFLEG"
BASE COUNT          611 a          898 c          834 g          535 t
ORIGIN      
        1 agcgcttcaa gacgaagtga acgaataata tcgtctcgca cgcgctgggt ggcggtagcg
       61 gtgtaggcgt ggatgcgggc ctggggaaat tgttcccgca ggcagctcag ggcgcggtag
      121 ctgggccgga aatcgtggcc ccacatgctg atgcagtggg cttcgtccac aacgaaatag
      181 gcgacccgct gcgtacgcag aaattcgagg aagtacgttt gcgtgaggcg ttccggcgcg
      241 gcatagagca ggcgcgtccg gccggagcgc aggtctgcga acacgtttcg ctgttcttcg
      301 ggggattgag cgctgttcag gcaatgcgca gggactcctc gttgccggag ggcgtcaacc
      361 tggtccttca tcaaagagat caacggggac acaacgaccg ccagcccctc cagggccagc
      421 gccggcgcct ggtaacacag cgacttgccc ccacccgtcg gcaataccac cagcgaatcc
      481 cggccttcca gaatggcctc gacggcttcg tgctgaaatg ggcgaaaact ctcgaatccc
      541 cagtattcct ggagtatggg atgtatgcgc ttcatagggg gttgcagggt accagattag
      601 ctgcccgcat cccaaattcc ccgtgcttcc cgcgatgagg aaggcaattc agtacctgcc
      661 gcattctgca agcgcttcgt acatggcgac gatgttttcg ggcggtacgt cgctctggat
      721 attgtggatg ctgctgaata cgtagcctcc gcccggcgcc agggcatcca cgttgcgttt
      781 gacgtcgtcc ttcacctcct gcggcgtgcc gtggggcaaa atatgctgag tatcgacacc
      841 cccgccccaa aacgtgatcg catccccgaa atcgcgcttg agggcgcgcg gttccatgcc
      901 cgccgcggtc acatgcaccg gattgatgat gtcgacgccc gcttcgatga atcccggcag
      961 gtagggccgc acattgccgc aggaatgaag aaacagccgg acatgcggcg cacaccgctt
     1021 gatcgaccgc agcacccgct ccaggtgagg ccggatcgtc gacgtatact gctcaaatga
     1081 aatgagctgg gactgctgcg tgccgtagtc gtcgttttcg accacgatat ccacttccgg
     1141 ccccaaagca cgcagcgcca tctcccagaa cgcgatcttg aggtctgcaa gcctgccgta
     1201 caggcaatct gcaaacgcgg gatagaccag gggatccatc agagccaact ccatgccgcg
     1261 cagccgctgg gccatctcaa agatgcccgc gcacaggcct ttgagcatga ccgccctccc
     1321 ctgcccacga aaacgggccg catggtcgcg caaaccgtgt attctctgcg gatccgcggc
     1381 gctcggccac ggatacgccg cgatcgttga aggttcaggc ggcagttccg ccagcggatg
     1441 cttcactata ctgaaccaca aaccgttttc cttcggaaaa tgctgtacca tcccccactc
     1501 gtcgcgatag acccagaccg ccccgccgtc ctccagctgg ccgaaaacgt cccaattgtg
     1561 gctcgtcaac ggaaacagcc cccgcgtatc cacgccaaaa cggtccagaa aagcgtccga
     1621 ggggatcact gtctgctgca ccacatcttg ccactcgggc gtttcgtcag acagcccgag
     1681 atacgcgcac acggcctcgt acgcctttcg ggaaatgccc gtgacatggg tgccggcaag
     1741 gtccagaggc acccgatcgg gttcgcggtg ggcaatcgcc gccaggatcc ggtcgcgcga
     1801 ggtcatattg agcacactcc ctccctcaat ccttcagaaa aacccggcac acggcggttt
     1861 ccgccagacg gaacgtcccg gaacgcatca atcacgtgaa ccggaccgcc tttcccgccc
     1921 ttcacgcgcc ggccagccct atctccgtgg ccgggcgatc gtacgggctt tttcaggata
     1981 cgcgcaagtt gaaaatccct ccagcaattt ccggcctgag ccgggaacca aacccgcgct
     2041 ccggagatag acataggcgt gtggttgtgc ttgttggggg tcaacacgta ccatagagtg
     2101 gcacactggg ccagcaaaca gccgcgatgg ggttgaccgg agagaataga caggcgatac
     2161 gaaagaaggg gctattccat gaaaacatct gcatgccttt tggttttggc ctccattatc
     2221 attctggtca tccccgcgtg ggcgaaaccc aatgaagtcc ccttgcccct gggaaagcgg
     2281 ctgatcgaat acggctggga cgtgcctacc acggaacagg tgcgcaaaaa catcaacaaa
     2341 atggagcgcc ggccctttga cgggctcgtg ttccgcctca aaggcggcgg caacgtcttc
     2401 gtgcccgagc cttggggcga gtcgcgcttc aaagaagact acgataacgc cgaacgcatc
     2461 aaatgggaac gctttacaga caacttcgtg atcatgtggg cggcctcgga tcaggactgg
     2521 ttcaatgatt cacaatggga gcagatcatc atcaataccc gcctggtcgc gcatgccgcc
     2581 cggctggcca gatgcgtggg gatctgcttt gacgccgagc cttacgggga aaatccctgg
     2641 gaatacgcaa agacggcgca ccacgacacc tattcgtttg cggagtacca ggccaaagtg
     2701 cgccagcggg gcgctcagtt cattcaagcg atcgaggcgg agctgccgga cccgcagatc
     2761 ctgacctttt tccagttgag ctattttgcc aacctctgcg tgcccatgga ccccggattc
     2821 cggcaggaga agctgtcaaa agagtactat gcgctgctgc ccgcctttct cgaaggca
//
LOCUS       DAOPBQ010000489         2866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_27520_length_2866_cov_3.797937, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000489 DAOPBQ010000000
VERSION     DAOPBQ010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27520_length_2866_cov_3.797937"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PKW60_13255"
     CDS             <1..779
                     /locus_tag="PKW60_13255"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPK00447.1"
                     /translation="QRVVALAHDPLSTMADIANAINQDPVVATKVLRLANSVAFGGTQ
                     EITGLDQACTRLGLKEIVRTVQAIAYSGLYRSTKPSVRETLQALWRHTVATSEAAHEL
                     ARIRCPESADEMFMAGLVHDLGAVLLINIIAHSPVTSASRLRTEREAAHDFEVKYHAL
                     AGIHVVSERNLPNTYAFTTFFHPNPADVPAEEHREAVLLVRAAERLAETCGYGFDGGL
                     EPVSADDPDLAELGLSAEDLERLQAQVSGAVESLLDVVSV"
     gene            complement(800..2557)
                     /locus_tag="PKW60_13260"
     CDS             complement(800..2557)
                     /locus_tag="PKW60_13260"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HPK00448.1"
                     /translation="MARSTRRSFLKNMGLGAGALAAACRHQSPDVARPAAPVRMDAGW
                     ERPNVIVILTDDHGYGDVGVHGNADIHTPHLDRFAKDGLDFTRFYCSPVCAPTRASLM
                     TGRYAYRTGVIHTARGGAKMHGDEITLAEMLRAAGYATGLFGKWHLGDNYPMRPQDQG
                     FDVCLWHKAGGIGQSPDNPNSYMNPRLWRGSEPVEAQGYCTDVFFSAAREFMGASRDR
                     PFFVYLATNAPHVPLEITQEYVAPYAAKGLDDSTARAYGMITNIDDNVGRLLAWLERE
                     KLRDNTLVIFLGDNGPEQERFTAGLRDRKVSVYEGGIRVPCFMQWPAVLGRQGRVEAV
                     AAHIDLAPTILEACKAVPPPEAVFDGISLLPLLRGATKALPERSLVFQCHRGLSPERY
                     HNAAVVAARYKLVCNPGTFHREYLDAEQAHAFELYDLSQDPGEQQNVAGAHPGTVAAL
                     RAVYDAWYDDMKATRNFEPGWIHLGSPFENPVQLCRFQDQAHLGERPLGWPVRVERGG
                     VYEFTLDRGGSTDAGTLHAAFQDNEMVRVLDDGERSALFTLPVGSGLLRVWMLRPGKP
                     VDLATNNDTTGDVTVRFLG"
     gene            complement(2577..>2866)
                     /locus_tag="PKW60_13265"
     CDS             complement(2577..>2866)
                     /locus_tag="PKW60_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00449.1"
                     /translation="GWKTFEVKKKNMLELLHRLRPGVTQVIVHCTRPSENFQFISDSG
                     DTRLADLEAMLDPDIRNAIEEEGIILTTWRELIERRARVAAPQETPGNGAG"
BASE COUNT          492 a          892 c          966 g          516 t
ORIGIN      
        1 gccagcgcgt ggtggcgtta gcgcacgatc ccctgtcgac gatggcggac atcgcgaatg
       61 cgatcaacca ggatccggtg gttgcaacga aggtgctgcg gctggccaac agcgtggcgt
      121 tcgggggaac gcaggagatc acgggtctcg atcaggcctg cacccggctg ggcctgaagg
      181 agatcgtgcg gacggtccag gccatcgcct acagcggcct gtaccgctcg acgaaaccct
      241 cggtgcgcga gacgttgcag gcgctgtggc gccataccgt ggcgacgtcc gaggcggcgc
      301 acgaactcgc gcgaatccgg tgtcccgaat ctgcggacga gatgttcatg gcggggctgg
      361 tccacgatct gggtgccgtg ctgctgatca acatcatcgc ccacagtcca gtgacgagcg
      421 caagccgcct gcgcactgaa cgggaagctg cccacgattt tgaagtgaaa taccacgcac
      481 tggcggggat tcatgtcgtt tcagagcgga atctgccgaa cacgtatgcg ttcacgacgt
      541 ttttccatcc caaccccgcg gatgtaccgg cggaggagca tcgcgaggcc gtgctgctcg
      601 tgcgcgccgc ggaacgcctc gcggaaacct gcggttacgg attcgacggc ggactcgagc
      661 ccgtgagcgc ggacgatccg gacctggccg aactgggact ttccgccgaa gacctggaac
      721 ggctccaggc gcaggtgagc ggcgcggtgg agtcgctgct ggatgtcgtt tcggtctagg
      781 cgccagcgcc agcggccggt cagccgagga aacgcacggt cacgtcgccg gtggtgtcat
      841 tattcgtcgc cagatcgacg ggtttgcccg gccgcagcat ccacacgcga aggaggccgc
      901 tccccacggg cagtgtgaac agggcgcttc gctctccatc gtccagcacg cgcaccattt
      961 cattatcctg aaaggcggcg tgcagggtgc ccgcgtccgt ggagccgccg cggtcgagcg
     1021 tgaattcata cacgccgccg cgctccactc gcacgggcca tcccagaggc cgctcgccga
     1081 ggtgcgcctg atcctgaaac cggcacaatt gcaccggatt ctcgaaaggc gagccgaggt
     1141 gtatccagcc cggctcaaaa ttccgtgtag ctttcatgtc gtcgtaccag gcgtcgtaca
     1201 cggcacgcag cgcagccacg gtaccgggat gcgcccccgc cacattttgc tgttcaccgg
     1261 gatcctggct gaggtcgtac aactcgaaag cgtgggcctg ctctgcgtcc aggtattcgc
     1321 ggtgaaaggt gccggggttg cagaccagct tataccgggc tgcgaccacc gccgcgttgt
     1381 gatagcgttc cggcgacagt ccgcgatggc actggaagac cagggatcgc tccggaagcg
     1441 cctttgttgc gccgcgcagc agcgggagca ggctgatgcc gtcgaagact gcctccggcg
     1501 gggggacagc cttgcaggcc tcgagaatgg tcggcgcgag gtcgatgtgc gcggcgacgg
     1561 cctccaccct gccctgccgt ccgagcacgg ccggccattg catgaaacac ggcacacgta
     1621 tcccgccttc atacaccgaa accttgcggt cgcgcagacc cgccgtaaag cgttcctgtt
     1681 cggggccgtt gtcgccgaga aagatcacga gggtgttgtc gcgcagcttt tcgcgctcca
     1741 accaggcgag cagccggccc acattgtcgt cgatgttggt gatcatgccg taggcccggg
     1801 ccgtggagtc gtccaggcct ttggcggcat agggcgcgac gtattcctgc gtgatctcca
     1861 ggggcacgtg aggcgcattg gtggccagat acacgaagaa ggggcgatca cggctggcgc
     1921 ccatgaattc gcgcgccgcg ctgaaaaaga catcggtgca atagccctgc gcctcgacgg
     1981 gctcgctgcc gcgccagagg cggggattca tgtagctgtt gggattgtcg ggggattggc
     2041 cgatgccgcc ggccttgtgc cacaagcaga cgtcgaagcc ctgatcctgg gggcgcatgg
     2101 ggtagttgtc gcccagatgc cacttgccga acaggccggt ggcgtatcct gcggcgcgca
     2161 acatttcagc aagggtgatt tcatcgccgt gcatttttgc cccgccgcgc gctgtgtgga
     2221 tcacgccggt ccggtaggcg taccggcccg tcatgaggct cgcccgggta ggggcgcata
     2281 cgggagagca gtaaaaccgg gtgaagtcga ggccgtcttt cgcgaagcgg tcgaggtgcg
     2341 gcgtatggat gtccgcgttg ccgtgcacgc caacgtcgcc gtacccgtga tcatcggtaa
     2401 ggatcacaat gacgttcggg cgctcccagc cggcatccat acggacaggt gcggcgggcc
     2461 gggccacgtc cggggactga tgccggcacg ctgcggcgag cgcaccggcg ccgaggccca
     2521 tatttttgag aaagctgcgt cgggtggatc gagccatgcc gctccgtctc ccgggttcag
     2581 ccggcgccgt tcccgggtgt ttcctgcggc gccgctacac gggcccggcg ctcgatcagc
     2641 tcgcgccagg tggtcaagat aattccttct tcctcgatgg cattcctgat gtccgggtca
     2701 agcatggctt cgaggtcggc gaggcgggtg tctccggaat ccgagatgaa ctggaaattt
     2761 tcgctgggcc gggtgcaatg gacaatgacc tgcgtcacgc cggggcgaag cctgtgcagc
     2821 agctccagca tgttcttctt ttttacctca aaggttttcc agccgt
//
LOCUS       DAOPBQ010000490         2862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_27571_length_2862_cov_3.952618, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000490 DAOPBQ010000000
VERSION     DAOPBQ010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27571_length_2862_cov_3.952618"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKW60_13270"
     CDS             <1..275
                     /locus_tag="PKW60_13270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK00450.1"
                     /translation="IKILNAMAAGRPVIACRSAPGPITDGIDGVVIPDNDPHAFAEAI
                     LRLRRDRSLREYLGTAARETIRTQHSRALLVRRLEAVYDAVCGAAE"
     gene            441..2141
                     /locus_tag="PKW60_13275"
     CDS             441..2141
                     /locus_tag="PKW60_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPK00451.1"
                     /translation="MKRSILAWMLAGIVAVSGCSGEKRVAPEPAETLAPAESSDQSAP
                     VAPSTEPESQTQAVPSWPPVDASRFITASGLRFIDTEGREILLHGINVVDKSPRRNYL
                     SWHSEADFARLRDWGMNVIRLGIIWDGLEPEPGVYNDAYLAEMDQRVAWAANQGLYVV
                     FDMHQDLFSVRYSDGAPEWATLTDDQPHISQGDVWSDAYFTSPAIQRAFENFWANKPA
                     PDGVGIQDHYAAAWRRVAERYAGNPAVLGYDLMNEPFPGAAILLGQSHMIGKAAELLR
                     IRLGEDAPTAEQIIIMWLNADKRGELMRYMDDVEFFAQVMDASAAAYEEFEKTQLMGM
                     YQRVADAIREVDTSHILFLEPNYASNAGVYSHLQPLKMPDGRRDPQQAYAPHGYDLVV
                     DTPHLARGSNARVSLIFTRHRETAERLHMPMLVGEWGAFGGHGADVVPAAWGVVDVLE
                     TLCCSDTYWEYGEYLPGAAYLPALQRAIPVRLAGTLVSYDNDIRAGTFTCTWREDPAL
                     TAPTILYIPASLDAANKTVALTPEGSSYTTEAVAEGSANQYYIIPKAGVAAERTLTVR
                     "
     gene            complement(2167..>2862)
                     /locus_tag="PKW60_13280"
     CDS             complement(2167..>2862)
                     /locus_tag="PKW60_13280"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HPK00452.1"
                     /translation="MWVATSGTIKVSAPFVAGVSPTHGATGAVQFTSRNGTLGRLGIA
                     TKVLTIMRTTEIIRLRLPPTKDEGITFDTCGARAVLDSGFMTIEQFDLKSPTLAMAGK
                     GTIDFPNNDTHMTVDIHLLQMATQVLDIVKLGDVADEIRKQSSYRLAISGPPEDPKVS
                     VQGLTTGEGITGSVTGVARDAAKAGQQTVVDVIQGSAGILRDILGGGKTQQPTPQLEP
                     EADAPPQQPEPEP"
BASE COUNT          515 a          895 c          923 g          529 t
ORIGIN      
        1 ccatcaaaat tctgaacgcc atggccgcag gaaggcctgt tatcgcgtgc cgctccgccc
       61 caggccccat caccgacggc attgacggcg tggtcatccc cgataacgat cctcatgcct
      121 tcgcggaggc cattttgcgg ctgcggcgcg accgcagcct gcgcgaatat ttgggcaccg
      181 ctgcgcgcga aaccatacgc acccagcaca gccgtgcatt gcttgtacgc cggctcgaag
      241 ccgtgtacga cgcagtctgc ggcgccgccg agtagggcag ctcaacgggg ttcctggccg
      301 cgcagcggga ccggatgttt cttggaggaa gcaggggcga tgtgggatga tggctgaacg
      361 tccataccgg aggcgcccgg tgtcagccag ccgattcaga cgaacgggct ccgtacagcc
      421 aattgacagg agaattcgac atgaaacgct ccattctcgc ctggatgctc gctgggattg
      481 ttgcggtgtc cggttgcagc ggcgaaaagc gggttgcgcc tgagccggcc gaaacccttg
      541 ctccggcgga atcttccgat cagtcagcgc cggtggcacc ctcgaccgaa cctgaatccc
      601 agacgcaggc cgtccccagt tggccgcctg tggatgccag ccgcttcatc accgcgtcgg
      661 gcctgcgctt catcgatacg gaagggcgcg agattttatt gcacgggatc aacgtggtcg
      721 acaagtcgcc ccggcgcaat tacctctcct ggcacagcga agccgatttt gcccgcttgc
      781 gtgactgggg catgaacgtg atccgcctgg gcatcatctg ggatggcctg gagcccgagc
      841 cgggggtata caacgacgcg tatctggccg agatggatca gcgcgtagca tgggccgcga
      901 accagggcct ctacgtcgtt ttcgacatgc accaggacct cttcagtgtg cggtattcgg
      961 atggcgcgcc cgaatgggcc acgctcacgg acgatcagcc gcatatcagc cagggcgatg
     1021 tctggagcga cgcctatttc accagcccgg ctattcaacg ggcgtttgaa aatttctggg
     1081 cgaacaaacc cgcgccggac ggcgtcggca tccaggatca ctatgcggcg gcctggcgcc
     1141 gtgtggccga acggtatgcg ggcaatcccg ccgttcttgg atacgatctg atgaacgagc
     1201 cgttcccggg cgcggcgatc ctgctcggac aatcgcacat gatcggcaag gcggccgaac
     1261 tgcttcggat ccggttgggc gaggatgcgc cgacggctga gcagatcatc atcatgtggc
     1321 tcaatgctga caaacgcggt gaactcatgc gctacatgga cgacgtggaa ttttttgcac
     1381 aggtcatgga cgcatccgcc gcggcctacg aggagttcga gaagacgcag ttgatgggca
     1441 tgtaccagcg cgtcgccgac gcgatccgcg aagtggatac cagtcacatc ctcttcctgg
     1501 agcccaacta cgcctcaaac gcgggtgtgt attcgcacct tcaaccgctc aagatgccgg
     1561 atggccgccg cgatcctcag caggcctatg cgccgcacgg ctacgatctc gtggtggata
     1621 caccgcacct ggcccggggg agcaacgcgc gggtgagtct catcttcacg cggcaccgtg
     1681 aaacagccga acgcctgcat atgccgatgc tggtgggcga atggggggcg tttggcggac
     1741 acggcgcgga cgtggtgccc gcggcctggg gtgtggtgga tgtgctggag acgctttgct
     1801 gcagcgacac ctactgggaa tacggggagt accttcccgg cgcggcttat ctgcccgcgc
     1861 tccaacgggc gatccccgtg cggctggcag gaacgctggt ctcgtacgac aacgatatcc
     1921 gggcagggac tttcacctgc acgtggcgcg aagatccggc ccttaccgcc ccgacgatcc
     1981 tctacatccc ggcgagcctg gatgcggcca acaaaaccgt cgcgctcact ccggaagggt
     2041 cctcctacac cactgaggcc gtcgcggaag ggtccgcgaa ccagtattac atcattccga
     2101 aggccggggt cgccgccgaa cggacgctga ccgtccggta acggagtccc cgcgagatcc
     2161 gcccgcctag ggctcaggtt cgggttgttg cggcggcgcg tccgcctcag gctccagttg
     2221 cggcgtgggc tgctgggtct tgccgccgcc gagaatgtcg cgcagaatgc ccgcggagcc
     2281 ctggatcaca tcgacgacgg tctgctggcc ggccttggcg gcatcacgcg ccacgcccgt
     2341 gacggatccg gttatccctt caccggtcgt gagtccctgc acggacacct tgggatcctc
     2401 cggcggcccg gaaatggcga gacggtaact ggattgcttt cggatttcgt cagcgacgtc
     2461 gccgagcttg acgatgtcga gcacctgtgt ggccatctgc aggagatgga tgtccacggt
     2521 catgtgggtg tcgttgttcg gaaagtcgat ggttcccttg ccggccatgg cgagggtggg
     2581 gctcttgaga tcgaactgtt caatggtcat gaacccggaa tccagcacgg ccctggcgcc
     2641 gcaggtatca aaggtgatgc cttcatcttt agtcggaggc aggcgcaggc ggatgatctc
     2701 ggtggttcgc ataatcgtca gcactttggt ggcgataccc agcctgccca gcgtgccgtt
     2761 ccgtgaagtg aactgaaccg cgcccgttgc gccgtgcgtc ggactcacgc cggccacgaa
     2821 cggcgcggag accttgatcg ttccggacgt cgccacccac at
//
LOCUS       DAOPBQ010000491         2855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_27655_length_2855_cov_4.205714, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000491 DAOPBQ010000000
VERSION     DAOPBQ010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27655_length_2855_cov_4.205714"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            581..656
                     /locus_tag="PKW60_13285"
     tRNA            581..656
                     /locus_tag="PKW60_13285"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:614..616,aa:Ala,seq:ggc)
     gene            1063..1668
                     /locus_tag="PKW60_13290"
     CDS             1063..1668
                     /locus_tag="PKW60_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPK00453.1"
                     /translation="MTTKDSQDISQKALSLLFASSARAKVLRLFMLDPCREYYQRQIE
                     KSTGLAIRAVQRELERLSELGLLFRRVEGNRTYYQVDVDFPLFPELQGLILKAGDDFD
                     RLRAFAAMERGVIVALLDESKTKALCVTEGAGRLSRGAPGPYSVEVMTSDRFLELLSA
                     SPARLAPFLSTGVDLLGRRDGLIWRRIESAGYNVSKGRGVA"
     gene            1670..>2855
                     /locus_tag="PKW60_13295"
     CDS             1670..>2855
                     /locus_tag="PKW60_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00454.1"
                     /translation="MVYRHEIRQLCETWWETLAYSPQAEQVRYAEEFLRLLEWDSAEQ
                     IQPAGVLDSTAATYLLCAEGRPSVMACFQAPGSLEPPSGLAERNLDFCGATRVLVTTA
                     KGLRAKYALITDLFRSYLYDTYTEELLLSADTPAEFAREMEETLCRAEVAAGSLEELR
                     RPPRSNVARQLREWCQRWQQTLVAQGHVREDAAELALDRILVLRFLTGHNVLRRHGHA
                     MRRRLNEIVARACMAGGDDCGRDLIRLFHDLWVELRADIFAPEPTLEKALERGAITVP
                     LLQELRLLARSKFTIPVILEKFNFGDASEKARVRMVPEDDEERLAYLYKQTSATIEDA
                     RITVDVEDEGYRAIFWWLDRLVALYDRLGQEYEHQSLNEQAGGDIDLFTWSELDAAKP
                     AIA"
BASE COUNT          571 a          771 c          943 g          570 t
ORIGIN      
        1 gtcatcgcag tgctcatcgc caggaaagac tcgccgcggg tttagcgtca atcgacttga
       61 gcggaataca gagctgagtg ggagttaagg tgtagaagag cttggcgtgc ctcgaaaatg
      121 aggccgcagc ttactgcaaa tatatgactt aatacaatca gctgatttcg gcacgagtga
      181 gtcacaggcc tgttctccga aaactgcacc agttggagtg actgccctcg taggctatgg
      241 ggcaaagaag gactctcaga tgaagggaaa gcgctcatag cgaagaacag atcgtacgaa
      301 ttatggagga ggtgcacgac gggaagccgg ttgtcgaagt ctgccgcgcg tatggtgagc
      361 ggagtggagg cggcccccgg ggccgcccat ccgcatagtc tccgcgctgc ggaaaccagt
      421 tccccgccgc cgctcatggt gagcgacgga cgcctccgac ggaaaagggc taaacccaaa
      481 aaccgtgcac ttagcattag aatgcgccca attcaaaaac ttgactccca tgcccgcatt
      541 ttctatactc cagccgcttc gagacagagc atctgaattt ggggatgtag ctcagttggg
      601 agagcgcttg tctggcagac aagaggtcag gggttcgatt cccctcatct ccaccatcta
      661 acatacgaca ggccagtggc cggcacacgc ccgccgctgg ccgttttttt tgtgctcggg
      721 cgacgcatgc gccgcccgta tggtgccctt cggcagggga tttgttccgc tgagcaaacc
      781 ctgtttgtgc tgatgtccct ggtggggcga cacatgcgtc gcccctacat gatctgcccc
      841 gcgcacggga tacgtcgctc gtatggaatg tgtcgcctcg caaggcacag attcagggca
      901 ggatactgct catttttaca tagaccccca caaaccaagc ttcgatcaat ttagttattg
      961 acagatatga ctacgatttg tatacttcga atacgtggag ggtagatacc gcagccagtc
     1021 tggcgtccct tcgcgcgagt aagatccggc acgaggttgt agatgacgac aaaagacagt
     1081 caagacatct cgcaaaaggc actttccttg ctgtttgcgt catcagcacg ggcgaaggtg
     1141 cttcgcctgt tcatgctgga tccctgccgt gagtattatc agcgacagat cgagaaatct
     1201 acaggcctgg cgatccgtgc ggttcagcgg gagctggagc gcctgtctga gctgggcttg
     1261 ttgttcaggc gggtggaggg caaccggacg tattaccagg tggatgtcga ttttccgctg
     1321 tttcccgaat tgcaggggct gattttgaag gcgggcgacg attttgaccg gttgcgggcg
     1381 ttcgcggcga tggaacgcgg cgtcatcgtg gcgctgctgg acgagtcgaa aacgaaggcg
     1441 ctgtgcgtga cagagggcgc ggggcggttg agccgtggcg cgccggggcc gtacagtgtc
     1501 gaagtgatga cgtcggatcg ctttctggaa ttgttgtcgg cgtcgccggc caggctggcg
     1561 ccgtttttgt cgacgggggt ggatcttctg ggacggcggg atgggctaat ctggcggcgc
     1621 atcgaatcgg ccggatacaa cgtgagcaag gggagaggtg tcgcatagca tggtttacag
     1681 acacgaaatc cgtcagttgt gcgagacgtg gtgggagacg ctggcctatt cgccgcaggc
     1741 cgagcaggtc cggtacgcgg aggagtttct gcgcttgctc gaatgggaca gcgcggaaca
     1801 aatccagccc gcgggggttt tggacagcac cgcggcaacg tatctgctct gcgccgaggg
     1861 ccgcccgtcc gtcatggcct gttttcaggc gccgggttcg ctggaaccgc cgagcgggct
     1921 ggcggaacgg aatctggatt tctgcggcgc gacgcgcgtg ctggtgacca ccgccaaggg
     1981 gcttcgcgcg aagtatgcgt tgatcaccga cctgttccgt tcgtatctgt acgataccta
     2041 cacggaagag ctgctgctga gcgcggacac gccggcggaa ttcgcgcgtg agatggaaga
     2101 gacgctgtgc cgggcggagg tagcggccgg ttcgctcgag gagctgcgcc gcccgccgcg
     2161 cagcaacgtg gcccgccagc ttcgggaatg gtgtcagcgc tggcagcaga cgctggtggc
     2221 acaggggcat gttcgggaag acgccgcgga gctcgcgctg gatcgaattc ttgtgttgcg
     2281 ctttcttacg ggacacaacg tacttcgccg ccatggccac gcaatgcggc gccggctcaa
     2341 cgagatcgta gcccgggcct gcatggcggg cggagacgac tgcggccgcg acctgatccg
     2401 cctctttcac gatctgtggg tggaactgcg cgccgacatt ttcgcgccgg agcctacgct
     2461 ggaaaaggcg ctggaacgag gcgcaatcac agtcccgctg cttcaggaat tgcgcctgct
     2521 tgcccggtcc aagttcacga ttccggtgat cttggaaaaa ttcaattttg gagacgcatc
     2581 ggagaaggcg cgggtgcgga tggtgcccga ggacgacgag gagcggctgg cgtacctgta
     2641 caagcagacg agcgcgacga tcgaagacgc gcgcatcacg gtggatgtcg aagacgaagg
     2701 ataccgcgcg atcttctggt ggctggaccg tctggtggcg ctctatgacc gcctgggcca
     2761 ggaatacgaa caccagtcgc tcaacgaaca ggccggcggc gatattgacc tgttcacctg
     2821 gtcggaactg gatgcggcga agccggccat tgcat
//
LOCUS       DAOPBQ010000492         2817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_28135_length_2817_cov_2.915641, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000492 DAOPBQ010000000
VERSION     DAOPBQ010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28135_length_2817_cov_2.915641"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PKW60_13300"
     CDS             <1..728
                     /locus_tag="PKW60_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041734229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPK00455.1"
                     /translation="ITEEQARATMQQCIPHGMMLLHKGKAQGLVAGASHSTGDTLRPA
                     LQILKTAPGARLASSFFFMAFEDATYLFADSGLVEDPNEEQLAEIAVATAQSALTFGI
                     DPTVAMLSYSTKGSAKSPLTEKVVKATELAQKRALEVFGPDSKVRIDGELQGDAALSD
                     RVGRSKAPGSEVAGRAKVLIFPNLDAGNIAYKLVQYLGGAEAYGPIIQGTRLPINDLS
                     RGCSPEDIVGVAAITVVQSQVLQ"
     gene            790..2049
                     /locus_tag="PKW60_13305"
     CDS             790..2049
                     /locus_tag="PKW60_13305"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPK00456.1"
                     /translation="MKVLVINSGSSSVKYKVYEHDTPPRMIARGIVERIGLSGPIMSC
                     GCCLTHYETCANQEILREPGGLKIRNHSDAIDALCQLLLSDSCGIIQDLSEIEGVGHR
                     VVHGGEEFTGSTIIDDAVLAGIQKCAKLAPLHNPPAILGIEACKKIFKNIPQVAVFDT
                     AFHHTIPRNAYLYGIPMGFYDEHGVRRYGFHGTSHRYVSQKAIELLDKPADKTRIITC
                     HLGNGCSITAVKGGKSVDTSMGLTPLEGVMMGTRSGDMDPAIPLFLIRELGISADEVD
                     AILNKQSGFKGICGKADVRDIERLAAEGDAACKLALNMFSYRVARYIGSYAMVMNGVD
                     AIVFTGGIGERGAAMRERILQNAGYLGLKIDLDKNAQSATDISTPDSQVRVFIIPTDE
                     ELVIASDTGRLVEEARGSGSREAALVK"
     gene            2621..>2817
                     /locus_tag="PKW60_13310"
     CDS             2621..>2817
                     /locus_tag="PKW60_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00457.1"
                     /translation="MKSTSIALIVSLFIVGSAVAAPLGGTYNSLTDPSIVTGSLGGTY
                     NSLTDPSIVTGAESLWDDELES"
BASE COUNT          621 a          807 c          809 g          580 t
ORIGIN      
        1 gaatcacgga ggagcaggcc cgggccacca tgcagcagtg cattcctcac gggatgatgc
       61 tgcttcataa aggcaaagcc caagggctcg tggcgggcgc ctcgcattcc acgggcgaca
      121 cgctgcgccc ggcgctgcaa attctgaaaa ccgctcccgg ggctcggctg gcctcatcat
      181 ttttcttcat ggccttcgaa gatgcgacgt atctgtttgc cgacagcggg ctggtcgagg
      241 atccgaatga ggagcagctg gctgaaattg ccgtggcaac ggcccagtcc gcgctcacgt
      301 ttggcatcga cccgaccgtg gccatgcttt cctattctac caaaggatcg gcaaagagcc
      361 cgctcacgga aaaggtggtc aaggccaccg aactcgccca gaaacgggcg cttgaggtat
      421 tcggcccgga ctcaaaggtc cggatcgatg gggaactcca gggcgatgcg gcgctttccg
      481 accgcgtcgg ccgcagtaaa gcgccgggca gcgaagtggc ggggcgcgca aaggtcctga
      541 tatttccaaa cctcgatgcg gggaacattg cctacaaact agtgcagtat ctcggcggcg
      601 ccgaggcgta cggccccatc attcagggaa cccggttgcc gatcaatgat ttgtcgcgcg
      661 gttgctcgcc cgaagatatc gtgggcgtag cggccatcac cgtagtgcag agccaggtgt
      721 tacaatagag ggcgagtccc gcgcgcctgc accgggcgta ttgcggctca tgttgtggag
      781 aaagcatcta tgaaggtcct cgtgattaac agcgggtcgt cgtcagtaaa atacaaagtc
      841 tacgaacacg acacacctcc ccggatgatt gcccggggca tcgtggagcg catcggcttg
      901 tcaggcccga tcatgagctg cggatgctgt ctgacgcact atgaaacctg tgccaaccag
      961 gaaatcctgc gggaaccggg cggattgaag atccggaatc actccgatgc gatcgatgcc
     1021 ctgtgccaat tgcttctgag tgattcgtgc ggcatcatcc aggacctttc ggagattgaa
     1081 ggagtgggcc accgcgtcgt tcacggcggc gaagaattca ccggctccac gatcatcgac
     1141 gacgccgtcc tggcggggat ccagaaatgc gcgaaactgg cgccgctgca taacccgccg
     1201 gccatcctcg gtattgaagc ctgcaagaag atttttaaaa acatcccgca ggtggcggta
     1261 ttcgacacgg cgttccacca caccattccc cggaatgcct atctctacgg aatacccatg
     1321 ggattttacg acgagcacgg cgtgcgccgc tacggattcc atggcacatc gcaccgctac
     1381 gtctcgcaaa aggccatcga attgctggac aaacccgcgg acaaaacccg aatcattacc
     1441 tgccacctgg gcaatggatg cagcattacg gccgtcaaag gcgggaaatc ggtggacacc
     1501 agcatgggtt tgaccccgct cgagggcgtg atgatgggca cccggtcggg cgatatggac
     1561 ccggctatcc cgctcttttt gattcgtgag ctgggcatca gcgccgacga ggtcgatgcc
     1621 attctgaaca aacaaagtgg attcaagggc atctgcggca aggccgatgt tcgtgatatt
     1681 gaacggcttg cggccgaagg cgatgccgcc tgcaagctcg ccctgaacat gttctcctat
     1741 cgcgttgccc gctatatcgg cagttatgcc atggtcatga acggcgtgga cgccatcgtc
     1801 ttcacgggcg gtattggaga gcgtggggca gccatgcgcg agcgcattct ccagaatgcc
     1861 ggctatcttg gactgaaaat cgatctggac aagaacgccc aatccgcaac agacatttct
     1921 acgcccgact ctcaggtacg ggtgtttatc attcccacgg acgaggaact ggtcatcgcc
     1981 tccgacacgg gccggctcgt ggaagaggcc cgcggctccg gttcgcggga agccgctctg
     2041 gtgaaatagc ctcgtgagcc tggccatcgc ggccccaggc tccatatctg tgccgggaat
     2101 gttttttcac ggatcatgga ctcgatcccc cgactttcca acccgttggc tatgcgttcg
     2161 cttccgcatg ccgattggag cccagtaaac acttgttcca cttggagcgg agttcttttt
     2221 cacgcagggg cggatagatc tttacggctt atcacgcttg acgaacattt gatctcaatt
     2281 gttattcaat gttgaccaac ctcaccgaaa atcatctgtt ctgctgagca ttctttgata
     2341 ttttggatag gtgcctaacc taatccatat actacatcgg cactttctga taggcgacgt
     2401 atcggaggcg aggaagaggg acgcctctct taatttattg caaaacaagc acttacaaga
     2461 tcttgcgcga aaaagtaaag actgaaaaac tcccagattc cacccacatc accaatgcgg
     2521 cggcgcgttt tttcatttcg gcatgaattg tgcttaacct aaggcaacag ggggtgtccg
     2581 cgtgtgctca cgcggaccag aatacacaag gagttccaga atgaagagca caagcattgc
     2641 gttgattgtc agcctgttca tcgtggggag cgcggtcgcc gcaccgttag ggggcacgta
     2701 caattccctc accgacccga gcatcgtcac cgggtcgtta gggggcacgt acaattccct
     2761 caccgacccg agcatcgtca ccggggcgga gagtctctgg gacgatgagc tggaatc
//
LOCUS       DAOPBQ010000493         2816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_28145_length_2816_cov_4.747555, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000493 DAOPBQ010000000
VERSION     DAOPBQ010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28145_length_2816_cov_4.747555"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="PKW60_13315"
     CDS             <1..1360
                     /locus_tag="PKW60_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HPK00458.1"
                     /translation="LYRVAQPRTQASYKSWGGVQSHEAAAEETGRIEKELASIAEQAD
                     FPLQVLPVMKAASVDELAKLERSKADATILYPASGSGSLLQAGMDLPDPVLFVRHRSG
                     PLYYWYEALSVQYLRSDRPDFEARREGPTPVLSVHDVVVDDLGELAWRLRTLHAVHNL
                     LGARVVALGGAQGKYAPNAPEKARERYGMEIVEVSYDDFAPRLATALEDPGTVRCAET
                     WTDRYLAMPGTSFETERGFVVNAFVLYGLFKELMAEHRAAAFTINNCMSTIMPMSQTT
                     ACLSLSLMNDEGTLALCESDFVVVPPAVLLYYLCGSPVFMHNSTFPHGGLVTCAHCTG
                     PRRMDGTRYEPARIVTHYESDYGAAPKVEMPLGTKLSFLNPEYAVGRWVGCRGEVVDN
                     PFLEICRSQQDVRIEGDWKRLLNEVRDSHWLMVYGDCLRELGYAANRLGVTWDNISDV
                     TA"
     gene            1521..2264
                     /locus_tag="PKW60_13320"
     CDS             1521..2264
                     /locus_tag="PKW60_13320"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPK00459.1"
                     /translation="MMMTIAKTAVALLMASWSLVAACAGETAPACACCANAECMKPDK
                     DGFISMFNGTDLTGWDGKPGGWWVEDGAITSESTEANPCVQHHYLFWKPAQPGDFVMR
                     FQYRLTGGNSGVQFRSERRPDYDVWGYQADMEAGTEWTGCLFQHDRNGVVMRGYKAVI
                     AHDGTRQEEQFASSEELQKLIKPGDWNDYEIIAEGPRITLKINGALMCEVVDKDAKMS
                     RSRGFIALQMHPGPPMKVQFRNLRIKIQR"
     gene            complement(2275..>2816)
                     /locus_tag="PKW60_13325"
     CDS             complement(2275..>2816)
                     /locus_tag="PKW60_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00460.1"
                     /translation="YEDFRYRETTKITPESFLSKDKHAAYATRRWPDLDTTDTEPLVL
                     CYKMPDWDVRLCRMDLTERYVLLPGGGLEEAQVLAPTKELPVRLVPIDQPDGLLRQVE
                     LPGPLEPGTYAVHWGALDGVVDKDPRIFAFSVVDFTLSLPPKEEAARQQAEEARDGSE
                     PEAPTPRPRDSEEHLPDVD"
BASE COUNT          539 a          777 c          958 g          542 t
ORIGIN      
        1 gctctatcgc gtggcccagc cgcgcactca ggcgtcctac aaatcgtggg gcggcgtgca
       61 gtcgcatgag gcagccgctg aagagaccgg gcgcatcgag aaagagcttg cgagcattgc
      121 ggaacaggct gatttcccgc tgcaggtgtt gccggtgatg aaggcggcct ccgtcgacga
      181 gcttgcgaag cttgagcgat cgaaggccga cgcgaccatc ctgtaccctg cttccggcag
      241 cgggagcctg cttcaggcgg gcatggacct gcccgacccc gtgttgttcg tgcggcaccg
      301 gtcgggcccg ctctactact ggtacgaagc attgagcgtg cagtatctgc ggagcgaccg
      361 gccagatttt gaggcccggc gggaagggcc tacgcccgtg ctgtcggtcc acgacgtggt
      421 tgtcgacgat ctgggagagc tggcgtggcg gctgcggacc ctccacgccg ttcacaacct
      481 gctcggcgcc cgggtcgtgg cactgggggg cgcgcagggc aaatatgcgc cgaatgcgcc
      541 ggagaaggcg cgcgaacgct acgggatgga gatcgtggag gtcagctacg acgattttgc
      601 gccccggctc gcgactgcgc tggaagaccc gggtaccgtg cgctgtgcgg aaacctggac
      661 ggaccgttat ctcgcgatgc cgggtacttc gtttgaaacc gagcggggat tcgtggtcaa
      721 tgccttcgtg ctctacgggc tgttcaagga actgatggcg gaacatcggg cggctgcgtt
      781 cacgatcaat aattgcatga gcaccatcat gcccatgtcg cagacaacgg cctgcctttc
      841 gctgagcctg atgaatgacg aggggaccct tgcgttatgc gaatcggatt ttgtggtggt
      901 tccgcccgcg gtattgctct attacctctg cggcagcccg gttttcatgc acaattcgac
      961 gtttccccat ggagggctcg tgacctgcgc gcattgtacc ggcccgcgcc gcatggacgg
     1021 cacacgctat gagcccgcgc gcatcgtcac gcactacgaa tccgactacg gcgcggcgcc
     1081 aaaggtcgaa atgcctctgg gaacgaagct gagctttctc aatcccgagt acgcggttgg
     1141 gcgctgggtc ggatgccggg gcgaagtcgt cgacaacccc ttcctggaga tctgccggtc
     1201 gcagcaggac gtgcgcatcg aaggcgactg gaaacggctt ctcaacgagg tccgcgattc
     1261 gcactggctc atggtgtacg gcgactgcct gcgcgaactg ggctatgcgg cgaaccggct
     1321 cggcgtgaca tgggacaata tttcggacgt gaccgcgtag tttccggaaa tcggcgctgc
     1381 tgctgtgcgg atttccggcg gcatggcgca gttggagaag ggaacgggga gtgaagagat
     1441 tgcccggtct gatcagatgg gaatgcagaa ccgcgaaaga tggcgggctg aaatttgcaa
     1501 atggatgcag caggagacag atgatgatga cgattgcgaa aacggcggtg gcgttgctga
     1561 tggcgtcctg gagtctggtg gcggcctgtg cgggcgagac tgccccggcc tgtgcgtgtt
     1621 gcgccaatgc cgaatgcatg aagccggaca aggatgggtt catctcgatg tttaacggaa
     1681 ccgatttgac cggctgggac ggcaaaccgg gcggctggtg ggtcgaggac ggcgcgatca
     1741 cgtcggagag tacggaggcc aatccctgcg tccagcatca ttatctgttt tggaaaccag
     1801 cacagccggg agatttcgtg atgcggttcc aataccgcct gacaggcggc aattcggggg
     1861 tgcagttccg cagcgaacgc cgcccggact acgatgtatg gggatatcag gcggacatgg
     1921 aagcggggac ggagtggacc ggctgccttt ttcagcatga tcgcaacgga gtggtaatgc
     1981 gcggttacaa agcggttatc gcgcacgacg gcacacgtca ggaagagcag tttgcctcat
     2041 ctgaagagct gcagaagctg atcaaacctg gggactggaa cgattacgag atcatcgcgg
     2101 agggcccccg gatcacgctt aagattaacg gggcactgat gtgcgaagtg gtggataagg
     2161 atgcgaagat gtcccgatcc agagggttca ttgccctgca gatgcatccc ggtccgccga
     2221 tgaaagtaca gttcaggaat cttcgcatca aaattcagag atagccagac gcgctcagtc
     2281 cacgtcaggc aaatgctctt ccgagtcgcg gggccgcggt gtgggagcct caggttctga
     2341 gccatcacgc gcttcttctg cctgctgcct ggcggcttcc tcttttggcg gaagagaaag
     2401 ggtgaaatcc acgacgctga acgcgaaaat gcgggggtct ttgtcaacca cgccgtccag
     2461 agcgccccag tgcacggcgt aggtcccggg ttcgagcggc cccggcagtt ccacttgccg
     2521 aaggaggccg tccggctggt cgatgggcac gagccgcacg ggcagttctt tggtaggcgc
     2581 gaggacctgc gcctcctcaa gtccgcctcc cggaagcagg acataccgct cggtcagatc
     2641 catgcggcag agccggacgt cccagtccgg catcttgtag cagagcacga gcggttccgt
     2701 gtcggttgtg tccagatcgg gccagcgccg tgtggcatag gcggcgtgct tgtctttgga
     2761 gaggaacgat tcgggcgtga tcttggtggt ttcgcggtat cggaagtctt cgtaga
//
LOCUS       DAOPBQ010000494         2797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_28396_length_2797_cov_4.273523, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000494 DAOPBQ010000000
VERSION     DAOPBQ010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28396_length_2797_cov_4.273523"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="PKW60_13330"
     CDS             <1..1166
                     /locus_tag="PKW60_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00461.1"
                     /translation="AQGRVRLSTAINAGSESQARLVRRSLAEALELSGDHGVYYVCRD
                     HTRGLEYIYPGRQLAHEVLYAELDGYQNQVFLDFREVRDLDGTWGALAHDLGGRGVPS
                     MDAAHREITLKPMREALRQALDPSLLKALFSGGTDVLPGADARRVFARAMTGFLSALQ
                     DQLIRPLDLDGALSEILRSVDAAQQSRDLVTSLGVEQRVVDYLRESSEVAAIGPETWW
                     AVPALWAVLAPLGEAISQSELEARTAAWMDEWPVTGVVYDVYRALGLDHSQAMLGTGL
                     VKLMLRYQDLPLLLTVSERAELLEQMFDDPVFSDFLYVNPYEGKLWLSKEQLEKLMYW
                     LFFCSVAHVLADQIHVLENSAEAILSQYHRVHEVLAAADSVKYQLEPLLELLS"
     gene            complement(1133..2056)
                     /locus_tag="PKW60_13335"
     CDS             complement(1133..2056)
                     /locus_tag="PKW60_13335"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPK00462.1"
                     /translation="MNLETLRCFCAVIEEGSFRAAAERLHRSQPAISQQVKSLEKKLG
                     QVLLERKTCRMTPLGALLYDRARHILNEAEGLTRELKDFDETLAGELRVGTSDTTALY
                     VLPEVVRQFALAMPQTHLVMVNRPSGAIAEQVARGELDLGIVTLPVSNDELIERDLFE
                     QHLVLVVPASHTLASRNRVSLPELREEPFLLLNANTRTGSVLRDLFAREAFTPQVVLD
                     SGSFEVIKRYVAEGIGVAFLPRIAVTPDDQQLATVFVPGLPRVRIGVIQRRHAYHTKA
                     QQAFLGLLDAQRGRPRDKPAAQDSSSSSGSS"
     gene            2165..>2797
                     /gene="purM"
                     /locus_tag="PKW60_13340"
     CDS             2165..>2797
                     /gene="purM"
                     /locus_tag="PKW60_13340"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPK00463.1"
                     /translation="MIPKKPGLSYRDAGVNIDAADEALRNVKDIVKKTFTPNVLRDIG
                     TFGAMYQLDLANYKEPVLVSSVDGVGTKLKVAFAMNKHDTVGVDLVSHCVNDILVQGA
                     RPLFFLDYLAFGRLVPEVAVDVITGLSNGCRYAGCALIGGETAEMPGLYNEAEYDLAG
                     TIVGIVDRSRIVDGSAIQAGDSIIALRSSGLHTNGYSLARKICFEVEGLGI"
BASE COUNT          504 a          838 c          872 g          583 t
ORIGIN      
        1 gcgcgcaggg gcgtgtccgc ctgtctacgg cgatcaacgc cggttccgaa agccaggccc
       61 gcctcgttcg gcgctcgctc gccgaagccc ttgaactgag cggcgatcac ggcgtctatt
      121 atgtctgccg cgatcatacc cgcggactcg aatacatcta cccgggccgg caattggccc
      181 acgaagtgct atacgcggag ctggacggct accagaacca ggtgtttctc gatttccgcg
      241 aagtgcggga cctggacggc acctgggggg cgcttgcaca cgatctgggc ggccgggggg
      301 tgcccagcat ggacgcggcc caccgcgaga tcacgctcaa accgatgcgc gaggcgctgc
      361 ggcaagccct ggatcccagc ctcttgaaag ccctgttctc cggtggcacc gatgtcctgc
      421 ccggcgctga cgcccgccgt gtttttgccc gagccatgac gggcttcctt tccgcgctgc
      481 aggaccagtt gattcggccg ctcgatcttg acggcgcgct gagcgagatc ctgaggtcag
      541 ttgatgccgc acaacagtcc cgcgatctgg tcacctccct gggcgtcgag cagcgggttg
      601 tggactatct tcgggagtcg agcgaggtgg cggctatcgg tccggagacg tggtgggctg
      661 tccctgcgct gtgggccgtt ctggcgccgc ttggcgaggc catcagccag agcgagctcg
      721 aggcgcgcac cgccgcctgg atggacgaat ggcccgtgac gggtgtggtt tacgacgtgt
      781 atcgcgcgct ggggcttgac cactcgcaag ccatgctggg cacggggctc gtcaagctca
      841 tgctgcgcta ccaggatttg ccgctgctct tgactgtgag cgaacgcgcc gagttgctcg
      901 agcagatgtt tgacgacccc gtcttcagcg attttctgta cgtcaatccc tatgagggca
      961 aattgtggct cagcaaggag cagctggaga agctgatgta ttggctgttt ttctgttccg
     1021 ttgcgcatgt gctggctgac cagatccatg tgctcgaaaa cagcgctgaa gccattctca
     1081 gccagtacca ccgggtccat gaggtgctcg cagcggcgga cagcgtcaag tatcagcttg
     1141 agccgctgct tgaactgctg tcctgagcgg ccggtttgtc cctgggccgg cctcgctggg
     1201 cgtccagcag tcccaggaag gcctgttgcg ctttggtgtg gtaggcgtgg cggcgctgaa
     1261 tcaccccgat gcgcacccgc ggcaggccgg gcacgaacac cgtggccagc tgctgatcgt
     1321 cgggcgtaac cgcgatgcgc ggcaggaaag ccactccgat gccctcggcg acgtaccgct
     1381 tgatcacttc gaaactgccg ctgtccagta ccacctgcgg ggtaaatgct tcccgggcga
     1441 acaggtcgcg cagcaccgac cccgtgcgcg tattggcatt gaggagcagg aagggttctt
     1501 cccggagttc cggcaggctg acacggttgc gcgacgccag ggtatggctg gccgggacca
     1561 cgagcaccag atgctgttcg aacaaatcac gttcgatcag ctcatcgttg ctcaccggca
     1621 gtgttacaat ccccaggtcc agctctccgc gcgctacctg ttccgcgatg gcgcccgagg
     1681 gccggttcac catgaccagg tgcgtttggg gcattgcaag cgcaaactgc cgcacgactt
     1741 ccggcaggac atacagcgcc gtggtgtcgc tggtccccac ccgcagttct ccagccagcg
     1801 tctcatcgaa atctttgagt tcccgggtca ggccttcggc ttcgttcagg atgtgccggg
     1861 cccggtcgta caataatgcg cccaacggcg tcatccggca tgtttttcgc tccagaagca
     1921 cctggcccag ttttttttca agcgacttca cctgttggct tatagcgggc tgagaccggt
     1981 gcagccgttc agctgcagca cgaaagctgc cctcttcgat gacggcgcaa aaacagcgca
     2041 gcgtttcgag gttcatgata agctctcctt atagaatgta ttataacatt tcgcttgaat
     2101 tattgaatac ccgccggtat cgtaagagat atgtggtgca ttcagccgac acaaggagtc
     2161 tgacatgatc cccaaaaagc caggcttgag ctatcgtgac gccggggtaa atatcgatgc
     2221 cgcggacgaa gcgttgcgca acgtcaagga catcgtcaag aagacgttca ccccgaatgt
     2281 tctgcgcgat atcggcacat ttggcgccat gtaccagctt gaccttgcga actacaaaga
     2341 gcctgtgctg gtatccagcg tggatggcgt cggcaccaag ctgaaggttg ccttcgccat
     2401 gaacaaacac gacaccgtgg gtgtggatct ggtgtcccat tgtgtgaacg acatcctggt
     2461 gcagggcgcc cggcccctgt ttttccttga ttatcttgca tttgggcggc tcgtgcccga
     2521 ggtagccgtc gatgtaatca cgggattatc caatggatgc cgctatgccg gctgcgccct
     2581 gatcggcggc gagacagccg agatgcccgg cttgtacaat gaggcggagt acgaccttgc
     2641 ggggacgatc gttggtatcg tcgatcgcag ccggatcgtc gacggctccg ccattcaggc
     2701 aggcgactcg attatcgcct tgcggtcttc cggacttcat acgaacggct acagcctggc
     2761 gcggaagatc tgtttcgaag ttgaggggct cgggatt
//
LOCUS       DAOPBQ010000495         2795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_28429_length_2795_cov_3.812774, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000495 DAOPBQ010000000
VERSION     DAOPBQ010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28429_length_2795_cov_3.812774"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            609..1181
                     /locus_tag="PKW60_13345"
     CDS             609..1181
                     /locus_tag="PKW60_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK00464.1"
                     /translation="MKQSFSTSEVAKYCHVTPDTIRKWAEAGRIHVFKTPGGHRRIRH
                     EDLILFLRDNKIPIHADLRDAGTKILVADTEKSVVTVIQRFLERSQNSFQVAIATDGF
                     EAGHQIALFRPDVLFVDLHMPGFSGGNICGRIKGNPDYASTQIIAMVNSEDSGMMEVA
                     IEHGAALCLRKPFTPDDLRRALARVGVEVN"
     gene            complement(1260..>2795)
                     /locus_tag="PKW60_13350"
     CDS             complement(1260..>2795)
                     /locus_tag="PKW60_13350"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPK00465.1"
                     /translation="LLRFGPSLNHGDPDDLGLLYYANGYELSYDIGYGLGSTHVHCGW
                     ASSTVSHALVTVDESNQFEQPGSGGSLLLFADLPGIKLVQATSEMSYVASEVTEYRRT
                     LALMARGGYLVDLFRVAGGKTHDYSFGSIGTNLEPFGLDALEPREGSLAEGYDWGHSI
                     GIDGDIHGYPNKPYWNPPPGNGYGFFFDVRTGTPPALWGGTWTIDGEEPTTLRMHVAG
                     DPAQAIFASAPGLYPHMPLSSYVIARRQSSDGTPLESTFLSVYEPAVPGSSSPRLNSV
                     KRLDEQAVEVVRADGAADVVLLGDGSLDTSCGSVVFRGDALHLEGRGKELALVETLGC
                     AYLEIDGQILHEGPGAFEARVVDVDSPGRSITLDADVPEGLEGCVAVFSNPAWTRTTA
                     YHVAGAEGARLLLHANTLALGIGRVTGILGKKSIASEITHEYTRTVKKGHSTRFFDGK
                     RIVGAKGGETRVTATVPGTPLQVDVEDSAALTVGETFTYYDLGPGDTVRVAWPRVHLA
                     PAG"
BASE COUNT          580 a          841 c          791 g          583 t
ORIGIN      
        1 ctccccggcg ccgccggatt atcgggaatt tgcgccacac cttagcagaa attgcgggat
       61 gctctctacc catatctgtt gccgcgccgc cgcacggcgt cacgatgccc gatgccaacc
      121 cgggtccagt ccgatgcagg gcactcggct ggatgcgctt ccgttgaacc cggctctttc
      181 tcaaggcgca gcgaatcttc atttttcaag gccagcgctt ccaagcggat cttccgcctc
      241 ttgatttcag acttttcatg ccattcatcg aatttcgccg atacggtctc attagcggca
      301 accatttatc tgatcaagac atacgacaag caagcaagac taaaagctct tccattgcgg
      361 gcgttgtgat tgtttttgac gcgcagggta tgttagcatg ccgtttcata catcgcgcgt
      421 gggaagcccg atttttccag ctcaggactg aggttgggga tgggttttct tttcgtgagt
      481 ttactgcgga gttcgccgca gttatcggat ttgctatcct tttttggtag gatatacaat
      541 atacgtttac aacacgtaga ggagcctgct gcttcggtgc agcggcgaac cctggttaaa
      601 cggtcacgat gaaacaatca ttcagcacgt cagaagttgc caaatactgc cacgtcaccc
      661 cggacaccat ccgcaaatgg gcggaagccg gccgcataca cgtgttcaaa acgccgggtg
      721 ggcatcggcg catccggcac gaagacctga ttctgtttct ccgcgacaac aagattccta
      781 tccatgccga tctgcgcgac gcaggcacga aaatcctggt tgccgatacg gaaaaatcgg
      841 tcgtaaccgt gattcagcgc tttctcgagc gatctcagaa ttcgtttcag gtggcgatcg
      901 cgacagatgg ttttgaagcc gggcatcaga ttgcgttgtt ccggccagat gtgctgttcg
      961 tcgacctgca tatgcccggt ttcagcggcg gcaacatctg cgggcgcatc aaaggcaatc
     1021 ccgactacgc cagcacgcag atcatcgcga tggtgaattc ggaagattcg ggcatgatgg
     1081 aggttgccat cgagcatggc gccgccctct gcctcagaaa accgttcacc ccggacgatc
     1141 tgcgccgcgc actcgcgcgc gttggcgttg aagtcaactg attcccgcct gtccaaaaat
     1201 ggcgcgattg tccaggaagc acttcgggcc tgacaagaag gtcgttatct caaaccgctt
     1261 cagccagccg gcgccaggtg cacccgcggc cacgcaacgc gcaccgtgtc gccaggtccg
     1321 agatcgtaat aggtaaacgt ctcgcccacc gtcagcgccg cgctgtcttc gacatcgacc
     1381 tgcaagggcg taccgggcac ggtggccgtc acgcgcgttt cccctccctt ggcgcccaca
     1441 atgcgttttc cgtcgaaaaa ccgcgtggaa tggccttttt tgacggttcg ggtgtattca
     1501 tgggtgatct cgctggcgat gctctttttt cccagtatcc cggtaacacg gccgatgccc
     1561 agagccaatg tattggcgtg caggagaagc ctcgcaccct cagcccctgc aacatgatac
     1621 gcagtcgttc gcgtccaggc ggggtttgaa aaaacagcga cgcagccctc gagcccttct
     1681 ggaacatccg catcgagggt aatcgatctc cccggcgaat ccacgtccac tacccttgcc
     1741 tcaaaggctc ctgggccttc gtgaaggatt tgcccgtcaa tttccagata agcgcatccg
     1801 agcgtttcca ccagagccag ttccttgcct ctgccttcca ggtgaagcgc atcaccacga
     1861 aagacgacac tgccacagga cgtatcaagg ctgccgtcgc ccaaaagcac cacatcggca
     1921 gcaccgtcgg cacgcacaac ctcgacggct tgttcatcaa gccgtttcac cgaattcagc
     1981 cgcggcgagg atgaaccggg gacggcgggc tcgtataccg acagaaacgt gctttccaga
     2041 ggcgttccgt cgctggactg acgccgggcg atcacgtagc tggacagcgg catgtgagga
     2101 tagaggcccg gagcggaggc gaagatggcc tgtgccgggt ctccggccac gtgcatgcgg
     2161 agagtggtcg gctcctcgcc atcaatggtc caggtgccgc cccagagcgc ggggggcgtt
     2221 ccggtgcgga cgtcaaagaa aaagccgtac ccatttcccg ggggcggatt ccagtacggt
     2281 ttgttgggat agccgtggat atcgccgtca ataccgatac tgtggcccca gtcataccct
     2341 tcggcgagac tgccttcacg cggctccagc gcatccaggc caaacggctc gaggttcgtg
     2401 ccgatgctgc cgaaactgta gtcatgcgtc tttccacccg ccacacggaa caggtcgaca
     2461 agataaccgc ctcgagccat gagcgccagg gtacggcggt actcagtcac ctcgctggcc
     2521 acatagctca tttcactcgt ggcctggacc agtttgatgc cgggcagatc ggcgaaaagg
     2581 agcagactgc cgccggaacc gggctgttcg aactggttgg attcgtcgac agtgacgagc
     2641 gcgtggctga cagtgctcga cgcccagcca cagtggacgt gggtgctgcc caggccatat
     2701 ccgatatcgt aggagagctc gtaaccgttt gcatagtata agaggcccag atcatccgga
     2761 tcgccgtggt tcagactggg tccaaagcga aggag
//
LOCUS       DAOPBQ010000496         2794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_28444_length_2794_cov_3.086528, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000496 DAOPBQ010000000
VERSION     DAOPBQ010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28444_length_2794_cov_3.086528"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /gene="fmt"
                     /locus_tag="PKW60_13355"
     CDS             <1..707
                     /gene="fmt"
                     /locus_tag="PKW60_13355"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPK00466.1"
                     /translation="APDICLIAAYGRILKQPILDVPRYGYLNMHPSLLPKYRGPSPIR
                     TALLNGDDVTGITIMRLTLEMDSGDILLQEEQPILPEDNWITLSERLAAKGGELLVKG
                     VDQIAAGTARFTPQDDSKALYCRMFEKADGRIRWGSPARTIHNLVRAAVPWPVAHCSY
                     NGDVYRIHESEVVEGSWEEVPGTVLAVEKDRVLVATGDNALGILAIQAPGKRALPIRD
                     FLAGRSIRAGERFEDL"
     gene            711..2033
                     /gene="rsmB"
                     /locus_tag="PKW60_13360"
     CDS             711..2033
                     /gene="rsmB"
                     /locus_tag="PKW60_13360"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HPK00467.1"
                     /translation="MPIDPVRDAAIDALLRVFERGVFLDASLNKTLRRRALSDRGRRF
                     FTQLVYGTVRHKMLCDYVLQGLLKQPLEGLPAPIRTLLRMGIFQALFCRQVTHPALVH
                     TSVDLAKKRGHAGVARLTNAVLRRAPQQLDAISFPREPVERLSIEYSIPLWMVKRWVA
                     SHGLEGAGGLCRAFSIEAPVAARVNTLRTSPEALAEALLKSGVRTEKRTTVPEELTFT
                     EGPPPVRSKRFQEGDFMMQDPASMLPSHLLEPAAGEWVLDMCAAPGGKTTHIAALTGG
                     NARIVACDRRADRLGQIQENVERMGCPGVRLVQADGGQPPFGARFQRVLVDAPCSGLG
                     TLRRHPDLKYRLLEKDIADLSRLQQELLRAAIERCENGGVIVYSVCTFTPEETGEVIA
                     RVTERAPVEPEDGPTWMDVWRLETGRYQTSPAQDGLDGFFLTRLRKRP"
     gene            1943..2737
                     /locus_tag="PKW60_13365"
     CDS             1943..2737
                     /locus_tag="PKW60_13365"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPK00468.1"
                     /translation="METRDGKVSNEPRAGRAGRVLLDTIAEATLNLAFFVFRFFGWSL
                     RWTLATVFFLLVMTAAGLYVFNTAVAGGEYVTVPNITLLSVTQASFELGRVGLEMGKQ
                     TPMFSDQVPPYHVISQRPEPGAVVRTGREVFPTVSQGAEIETTPEYVGKTLKEVESAL
                     APGMRLGNKAWIQHSTPRDQIIAQDPPAGRALHANARLAVDPLALGVDEHAAGPVLVG
                     PLPRARLSPTGNVAPRHADVVGIENVVLDVFSPRWRADLRECRAAG"
BASE COUNT          504 a          856 c          914 g          520 t
ORIGIN      
        1 aggccccgga tatctgcctg atcgccgcct acgggcgcat tctcaagcag cccattcttg
       61 acgttccccg ctatggctat ctcaacatgc acccaagcct gctcccgaaa taccgcggcc
      121 cctcccccat tcggaccgct ctgctgaacg gcgacgacgt gacggggatc acgatcatgc
      181 ggctgaccct ggagatggac tccggggaca tacttttgca ggaagaacag ccgatcctgc
      241 ccgaagacaa ctggatcacc ctgtctgaac ggcttgcggc gaaaggcggg gagcttctgg
      301 tgaagggcgt cgaccagatt gccgcgggaa ccgcccggtt cacgccgcag gatgattcca
      361 aggccctcta ttgccggatg ttcgagaagg ccgatggccg gatccgctgg gggagtcccg
      421 cacgcacgat tcacaacctc gttcgggcgg ccgttccctg gcctgtggcc cattgcagct
      481 acaacggcga tgtctaccgg atccatgagt ctgaggtggt ggaaggttca tgggaggagg
      541 tccccggcac cgtgctcgcc gtggaaaaag accgggtgct cgtagccacg ggggacaatg
      601 cgctgggcat tctggcgatc caggcgccgg gcaagcgcgc cctgcccatc cgggacttcc
      661 ttgccggtcg ttccatccgg gcgggagaac gattcgagga cctgtgagcc gtgcctattg
      721 atccggttcg agatgccgcc attgacgccc tgctgcgggt tttcgagcgc ggagtctttc
      781 tcgacgcgtc gttaaataag acccttcggc ggcgagccct gagcgaccgg ggccggcgct
      841 ttttcacgca gctggtctat ggcaccgtgc gtcacaagat gctttgcgat tatgtgcttc
      901 agggacttct gaaacagccg cttgagggcc tgcctgcccc gatccgcacg ctgttgcgaa
      961 tgggcatttt tcaggcgctc ttctgccggc aggtaaccca tcccgcgctg gtgcacacgt
     1021 cggtggatct ggcgaagaag cgaggccatg ccggtgtggc gcgcctgacc aacgccgtac
     1081 tgcgccgcgc gccccaacag ctcgacgcca tctcttttcc ccgtgagcct gtcgagcgcc
     1141 tgagcatcga gtactccatc ccgctgtgga tggtgaaacg ctgggtggcg tcacatggac
     1201 tggaaggcgc cggggggctc tgccgggcgt tttccatcga ggcgccggtc gcggcccggg
     1261 tcaacacgct ccgcacgagc cctgaggctc ttgcagaggc actgctgaaa agcggcgtgc
     1321 ggactgaaaa gcggacgacg gtgccggagg aactgacttt cacggaaggc ccgcctccgg
     1381 tgcgcagcaa gcggtttcag gagggagact tcatgatgca ggacccggca tcaatgcttc
     1441 cctcgcatct gctggaacca gccgccgggg aatgggtatt ggatatgtgc gcggcgcctg
     1501 gaggcaaaac gacgcatatc gctgcgttga ccggcggcaa tgcgcgcatt gttgcgtgcg
     1561 acaggcgcgc ggaccgcctc gggcagattc aggaaaatgt cgagcgaatg gggtgccccg
     1621 gagttcgcct ggttcaagcc gatggcgggc agccgccgtt tggcgcccgt ttccagcgcg
     1681 tactggtgga cgcgccctgt tccggcctgg gcacgctgcg ccggcatccc gacctgaaat
     1741 accggcttct cgagaaggac atagccgacc tgagcagact ccagcaggaa ctcttgagag
     1801 ccgcgatcga acgctgtgag aatggcggcg tcattgtgta ttctgtatgc acctttacgc
     1861 cggaagaaac cggggaagtc attgcgcgcg taacagaaag agcgccggtc gaacctgaag
     1921 acggcccaac ctggatggat gtatggagac tagagacggg aaggtatcaa acgagccccg
     1981 cgcaggacgg gctggacggg ttcttcttga cacgattgcg gaagcgaccc tgaatctggc
     2041 gttctttgtc ttccgctttt tcgggtggtc gttgcgatgg accctggcaa cagtcttctt
     2101 tctgctggtg atgaccgccg ccggtttgta tgtgtttaat acggcggtcg ccggaggcga
     2161 atacgttacc gtacccaaca ttaccctgct ttccgtgacg caggcttctt ttgagctggg
     2221 cagagtgggc ctggaaatgg gcaagcagac ccccatgttc agcgaccagg tgccgcctta
     2281 ccacgtcatc agccagcggc cggaacctgg cgccgtggtg cgcacgggcc gcgaggtgtt
     2341 cccgacggtg agccaggggg cggaaatcga gacaacgccc gagtacgtcg gcaaaaccct
     2401 gaaagaggtg gaaagcgccc tggcccccgg aatgcgcctt ggcaacaagg cgtggataca
     2461 acacagcacg ccgcgggacc agatcatcgc tcaggatccg cccgcaggcc gcgccctgca
     2521 cgcgaatgcc cgattggccg tcgatcctct tgccctcggc gtcgacgaac acgccgcggg
     2581 tccggttctc gtcggcccac tgccacgtgc ccggctctcc ccaaccggaa atgtcgcacc
     2641 ccgtcacgcg gacgtggtgg gaatcgagaa cgtggtcctc gatgtgttca gccctcgatg
     2701 gcgggctgat ctgagggagt gtcgcgcggc tgggtgagct cggccattga ggtgtcggag
     2761 aggtagcggc gggcgatggc ccactcgtcg tgct
//
LOCUS       DAOPBQ010000497         2791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_28486_length_2791_cov_3.991228, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000497 DAOPBQ010000000
VERSION     DAOPBQ010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28486_length_2791_cov_3.991228"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PKW60_13370"
     CDS             <1..531
                     /locus_tag="PKW60_13370"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPK00469.1"
                     /translation="QVLDSLGWRERVGKKPAVWVSVDEQVLRVLDGDAIIWESPCATA
                     AKGTGSEKDSYKTPLGWHEIFAKTGNGAPWGQVFRSGVPTAQVWKAGQRTREDLVLTR
                     IFFLGGLEPGRNKGGNVDSRARYIYIHGTNDEAAIGTPSSQGCVRLRNDDVIALYELV
                     PEGAKVLITETRQASD"
     gene            complement(577..>2791)
                     /locus_tag="PKW60_13375"
     CDS             complement(577..>2791)
                     /locus_tag="PKW60_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00470.1"
                     /translation="PDLPEVAQETEEASISIPEPVVWPEPPIYICLGNGAPELLRAKV
                     EQAAAELPVEEGLPVRFHRFIAEAPLPWPDHGISAEDSLAVLHAIAETCADAEIILSI
                     PLDPPAAWLEAHPESIMMIDGEKQPYVSVASKPWREAVRDSLETLISAIKEDPAGAQV
                     RGYLLRCLKDGQWVHAGGYDRSDANIAGFHDWLTVRYQTVEAFRAAWNNKELAMETAP
                     LPREVVSGDTHTVFHKYPGERPNVDYLEYTSKNTAETLALFASQIKALAGHDTRVHAP
                     YGLILGPMSGCEGHAALNELLSSEVDGFVSPTEYENSAIEQNGGILGAAHSALNQGKS
                     WLLLDDSLERASTAASGDAAAKALRQRLDHVFAAAVTQGMSLGWRIPIDTPEFPDTST
                     WNTLKSLKAMYSPLTEAKPVDGLDPFGGPENRLMTIVLGETGRFHQVCGTKINDDLLR
                     TMPANVLHAGVPAQICLFSDLLAGTVPATPCYLFLNTFYIPAENREALHALLRETGAT
                     AIWLYAPGYCNGEANAAANIAATVQMNVKPYEKPVPSGSVVTLGGVWIPTGTEFGNGT
                     GWSPSFYIEDDDVNVIAKHVGNGKASVAIAFPDGEDGDKGWTSVYCAEPVLPPGLLRE
                     ILRTLEIYQYIRSAPPESEDFYYFGRNSLAIHATTGGERIIDLGGVYNVQDLLDTAVG
                     WPEKRIMTVPLEAGQTRIFKIMPVPLANDALPGDAETSALDSALEEETEGPVPAA"
BASE COUNT          511 a          812 c          912 g          556 t
ORIGIN      
        1 caggtgctgg attctctggg ctggcgggaa cgcgtgggca agaaacccgc ggtctgggtc
       61 tctgtggatg aacaagtgct gcgggtgctg gacggggacg ccatcatctg ggaaagtccc
      121 tgcgcgaccg cagcgaaagg gaccggctcg gagaaggaca gctataaaac gccgctgggc
      181 tggcatgaaa tctttgccaa aaccggcaat ggcgcgccct ggggacaggt gttccggagc
      241 ggagtcccga ctgcgcaggt gtggaaggcc ggacagcgca ccagggaaga cctcgtgctc
      301 acgcggatct tcttcctcgg cggcctcgag ccgggacgca acaaaggtgg gaacgtagac
      361 tcgcgcgccc gctacattta catccacggg accaacgatg aagccgcgat cggcaccccg
      421 tcgtcgcagg gctgtgtacg gctgcgcaac gacgacgtca tcgcgctcta cgagctcgtc
      481 cccgaaggcg ccaaggtgct gattacggaa acccggcagg ccagcgacta attggaaagg
      541 ccaatgtggc cggtgtcgcc gtaaatggag gtttcgtcac gcggccggca cgggtccctc
      601 cgtttcctcc tcgagggcgg aatcaagggc gctggtttcg gcgtcgccgg gcagcgcatc
      661 attggccagg ggcaccggca tgattttgaa aatacgcgtc tggccagcct caagcggtac
      721 cgtcatgatg cgtttttcag gccatccaac ggctgtgtcg agaagatcct gcacgttata
      781 gaccccgccc aggtcaataa tgcgttcgcc gcctgtggtc gcgtggatcg cgagtgaatt
      841 gcggccaaaa tagtaaaaat cttccgattc cggcggggcg ctccgaatgt attggtaaat
      901 ttcgagcgtg cggagaattt cccgcaacag cccaggcggc aggacgggct ccgcgcaata
      961 gacggaggtc cagcccttgt ctccgtcttc gccgtcagga aaggcaatgg ccacgctggc
     1021 tttgccgttt cccacatgtt tcgcgatcac gttgacgtca tcgtcctcga tatagaacga
     1081 cggggaccag ccggtgccgt tcccgaattc cgtgccggtg gggatccaca ccccgcccag
     1141 agtgaccacc gaacccgacg gcacaggttt ttcatacggt tttacgttca tctgcacggt
     1201 cgccgcgatg tttgcagcgg cgttggcttc accgttgcag tatccgggcg cgtaaagcca
     1261 gatcgcggtc gctcccgttt cccggaggag cgcatgcagg gcctcacggt tttctgcagg
     1321 gatgtagaag gtgttgagga acagatagca cggagtggcg ggcacagtgc cggccagcag
     1381 gtcgctgaac aggcagatct gcgccgggac gccggcgtgc agcacgttgg ccggcatggt
     1441 tcgcagcaag tcgtcgttga ttttcgtgcc gcagacctgg tggaagcgcc cggtctcccc
     1501 caacacgatt gtcatcaggc ggttttcagg ccctccgaac gggtccaggc cgtcaacggg
     1561 tttggcttcc gtgaggggac tgtacatggc cttaagagat ttcagggtgt tccaggtgga
     1621 tgtgtcggga aattccgggg tgtcgatggg gatacgccag ccgaggctca tgccctgggt
     1681 caccgcggcc gcgaacacgt ggtcaagccg ctggcgcagc gcttttgcgg cggcgtctcc
     1741 gcttgcagcc gtgcttgcgc gttcgagcga gtcgtcgagg agaagccacg acttgccctg
     1801 gttcaatgcg ctgtgggctg cccccagaat gcccccgttc tgctcgatgg cgctgttttc
     1861 gtattcagta ggcgagacaa agccgtccac ctcgctgctg agcagctcgt tgagcgcggc
     1921 atggccttcg caaccgctca tgggacccag tatgaggccg tagggggcat ggactcgcgt
     1981 gtcatggcct gcaagcgctt taatctgaga ggcgaacagg gccagggttt ccgcggtgtt
     2041 tttggaggta tattcgagat aatcgacgtt gggacgttcc ccggggtatt tgtggaacac
     2101 ggtgtgggta tcgccgctca cgacctctct gggcagcgga gcggtttcca tcgccagttc
     2161 cttgttgttc caggcggcgc gaaacgcctc gacggtctga tagcgaacgg tcagccaatc
     2221 gtgaaatccg gcgatattgg catccgagcg atcatagccg ccggcatgca cccattgccc
     2281 gtccttaagg cagcgcagga ggtaaccgcg cacctgtgct cctgccggat cttcctttat
     2341 ggctgagatc agggtctcga ggctgtcccg gacggcttcc cgccagggtt ttgaggcgac
     2401 ggagacatag ggctgctttt ctccgtctat catcatgatg ctttcaggat gagcctccag
     2461 ccaggcggcg gggggatcga gcgggatgga gaggatgatc tccgcgtcgg cgcaggtttc
     2521 cgcaatggca tgaaggactg ccagcgagtc ttccgcagag atcccgtgat caggccaggg
     2581 caaaggggct tcggcgataa agcggtggaa gcggactggc aggccctctt caacgggcag
     2641 ttcagcagcc gcctgctcca ctttggcccg caggagttcc ggagctccgt tcccaaggca
     2701 tatatagatc ggcggttccg gccagaccac aggctccggg atgcttatgc tcgcctcttc
     2761 ggtttcctgc gccacctccg gcaaatcggg c
//
LOCUS       DAOPBQ010000498         2786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_28553_length_2786_cov_2.823508, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000498 DAOPBQ010000000
VERSION     DAOPBQ010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28553_length_2786_cov_2.823508"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..976)
                     /locus_tag="PKW60_13380"
     CDS             complement(110..976)
                     /locus_tag="PKW60_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00471.1"
                     /translation="MKIQRSLGRRWRAAPFSLVFVLLLTAAHTIPARELGQAELDAAP
                     TRVGDWQYADGAFVQDEPTTGLLALPLEPAARQTIHFRVQLSRDEEGAEAGVAFPMEG
                     GDFSIRCSVWRGKGAWYAGIVDYGPYRYGRMPGDQVRLPDYQPGQWVEMAVRIDGGRV
                     EAQVDAEYQLAASFPVKEGKPVSWKNMSEQGDPILEREHTVALRTVQTAARFDNLTIE
                     ALADGDELVTPLTPRYDENGRVQPRWDYAAALGAFTDWFARCGEFVSAAGGDPNADPL
                     EPVMVETVFPEL"
     gene            complement(1009..2250)
                     /locus_tag="PKW60_13385"
     CDS             complement(1009..2250)
                     /locus_tag="PKW60_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPK00472.1"
                     /translation="MQPSSLATALRCPVCDYERLREAMQVAGLDLVLANARRTVSWLT
                     DYQTVYWTWDHAILHMLDKEYDGRDYHVFAGFPREPGKHSFLVEYAHREQAVEQFGIH
                     ADRFYGYWTRGRIPEITGEGVTLERDWSRTSVEAVVQAIQDCGLDNATIGIEEGTLPL
                     NVFRDLRNLLPRVRFQDAGDLLFDLRQVKTFEEIRRFRHAYEVADRTYRRAFARAGTG
                     VTPRELLLEEMDEIYRSGCSFSFSHVHFSSGATDLAVTPPPDRPIEPGDVGLFDLGVV
                     YEGYSTDYARMAEVAPGNEKLRRHYPAILRAREVIERGIRPGVIADDLFTAGARVLED
                     AGLTASISCLGHGIGLGPCERPFLNAGSQDRIVPGQILVIEIYAEVGGLGPILMEDGG
                     LVTEAGWESFCGLPTDIVQLG"
     gene            complement(2261..>2786)
                     /locus_tag="PKW60_13390"
     CDS             complement(2261..>2786)
                     /locus_tag="PKW60_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPK00473.1"
                     /translation="SFAHIFFGSGQHDIAFTPPADRAIEPGDLGLFDLGVVYEGYGTD
                     YARMAEVAPGNEKLRRYFPAILDGRKAIEQALRPGAKAGDVFLAGAKVLEEHGLAASI
                     SCLGHGIGIGCHEKPFLVANSEDRIEVGQTLVIEMYAEVKGLGPLLMEDGGYMTEEGW
                     HSFSPLSTEILQLH"
BASE COUNT          541 a          942 c          822 g          481 t
ORIGIN      
        1 attacggctc cggcaacacc gtctccacca tcagcggctg cggcgacggc atcaacggcg
       61 gctacggcaa caccgtctcc accatcagcg gctgcgacga cggcatgatc tacagctccg
      121 gaaacaccgt ctccaccatc accggctcca gcgggtccgc attggggtcg ccgccggccg
      181 cgctgacaaa ttcgccgcag cgggcaaacc agtcggtgaa tgcgccaagg gccgcggcgt
      241 agtcccaccg cggctggact cggccgttct cgtcatagcg cggcgtcagc ggcgtgacca
      301 gttcatcgcc atcggcaagc gcttcgattg ttagattgtc gaaccgggcg gctgtctgca
      361 ccgtcctcag cgccaccgtg tgttcgcgct caagaatcgg atcgccctgc tcgctcatgt
      421 tcttccacga caccggcttg ccctctttca ccgggaagga cgccgccagt tgatactcgg
      481 catcgacctg cgcctccacc cggcccccat cgatacgaac cgccatctcc acccactggc
      541 ccggctgata atcgggcagc cgcacctgat cgcccggcat ccggccataa cggtaggggc
      601 cgtagtcgac gatgccggca taccatgccc ccttgccgcg ccacacggag cagcggatcg
      661 agaaatcccc cccctccatg ggaaaggcga cgccggcctc ggcgccctct tcgtcacggc
      721 tcagttggac ccgaaaatga atggtctggc gcgccgccgg ctccagcgga agcgccagca
      781 acccggtcgt cggctcgtcc tgaacaaaag ccccgtccgc atattgccag tcaccgactc
      841 gtgtgggtgc cgcgtcaagt tccgcctgcc ccaattcccg cgccgggatg gtatgagcgg
      901 ctgtcagcag aagcacaaat accaggctga atggtgcggc gcgccagcgt cgcccaaggg
      961 atcgctgaat tttcacggtt cgcctccttt cggtgcgcac ggttcggttt accccaactg
     1021 gacgatatcg gtcggcaggc cgcagaagga ctcccagcct gcttccgtca ccaggccgcc
     1081 atcctccatg aggatcgggc ccagaccccc cacttcagcg taaatctcga tcaccaggat
     1141 ctgccccggt acaattcgat cctggctgcc ggcattgagg aatggccgct cgcacggccc
     1201 caagccgatg ccatggccga ggcacgaaat ggaggcggtc aggcccgcgt cctccagcac
     1261 gcgcgcgccc gccgtgaaca ggtcgtccgc gatcacaccg ggccggatcc cccgctcgat
     1321 gacctcgcgc gccctgagaa tggccggata gtgacgccgc agcttctcat tgcccggcgc
     1381 cacctcggcc atgcgcgcgt aatcagtcga gtagccctca tacacgacgc cgagatcgaa
     1441 cagcccgaca tcgccgggct caatcggccg gtcgggcggc ggcgtaacgg ccagatcggt
     1501 tgcccccgag ctgaaatgaa catgcgaaaa gctgaacgag cagccgctgc ggtagatctc
     1561 gtccatctct tcgagcagga gttcgcgcgg cgtgacgccg gtgccggcac gcgcgaaggc
     1621 gcgccgatag gtcctgtcgg ccacctcgta tgcatggcga aaacgacgga tctcctcgaa
     1681 ggtctttacc tgccgcagat caaaaagcag atcgcccgca tcctgaaacc gcacgcgcgg
     1741 cagcagatta cggaggtcgc ggaagacatt gaggggcaga gtgccttctt caatgccaat
     1801 cgttgcgttg tccaggccgc aatcctggat tgcctggaca acggcttcga ctgaggttcg
     1861 ggaccagtcc cgctccagag tcacaccttc gccagttatt tccgggatgc ggccgcgtgt
     1921 ccaatatccg tagaagcggt cggcatgaat cccaaactgc tcgacagcct gttcgcggtg
     1981 ggcgtactcg accaggaatg aatgcttgcc tggctcgcgc gggaaacctg cgaacacatg
     2041 gtaatcacgc ccgtcgtatt ccttgtccag catgtggagg atggcgtggt cccaggtcca
     2101 gtacacggtc tgatagtcgg tcagccagga gacggtgcga cgggcattgg ccaacaccag
     2161 atccaatccg gcgacctgca tagcctcgcg cagtcgctca taatcacaga cggggcatcg
     2221 cagagctgtg gccagagatg agggttgcat tgcagcgctt tcagtgaagc tgaagaattt
     2281 cggttgataa cgggctgaag gaatgccagc cctcttcagt catatagcca ccatcttcca
     2341 tcagcagggg ccccagaccc ttcacctcgg catacatctc gatcacaagc gtctggccga
     2401 cttcgatgcg gtcttcgctg ttggcgacga ggaaaggctt ttcgtggcaa ccgatgccga
     2461 tgccgtggcc gaggcacgag atggatgccg ccagaccgtg ctcctcaagc accttggccc
     2521 cggccagaaa gacgtcgccc gctttcgccc caggtcgcag cgcctgctca atcgccttgc
     2581 gtccgtccag aatggccggg aaatagcggc ggagcttctc gttgccgggc gccacttcgg
     2641 ccatgcgcgc ataatcggtg ccataaccct catagacgac gccgagatcg aacaacccga
     2701 gatcgccggg ctcgatggcg cggtcggccg gcggggtgaa agcgatatca tgctggcccg
     2761 aaccgaaaaa gatgtgcgca aagctg
//
LOCUS       DAOPBQ010000499         2754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_28963_length_2754_cov_3.284920, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000499 DAOPBQ010000000
VERSION     DAOPBQ010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28963_length_2754_cov_3.284920"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2134
                     /gene="pheT"
                     /locus_tag="PKW60_13395"
     CDS             <1..2134
                     /gene="pheT"
                     /locus_tag="PKW60_13395"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPK00474.1"
                     /translation="KVPTAVDGSTLAGGFKITHRKMRGVESQGMMCAPDELGMGSDHS
                     GLLILPADAPVGADALHLLGLDDTVLEIEITPNRGDWASMIGVARELSALHKTPLRQP
                     QFKLQEDGPPASGYSSVTIEAPELCPRYAGRIITGLTIGPSPLWLRQRLLAAGQRPIN
                     NVVDVTNLVLLETCQALHAFDFNKLHENRIVVRNAKHNETIETLDEQTRKLTPEMLVI
                     ADARHPVALAGIMGGAASEVTDSTTDIFLESAYFQPATVRATARALGMNTEASQRFQR
                     GADPEMAIWALDRAAYLIQLVAGGAIAKGRLDEYPKPFETKTLQLRYARTDLLLGTAV
                     PPEEQRGILERLAFTSTATDETTCTVRIPSWRHDVGREADLIEEIARVYGYEKIAASL
                     PPVRQNELIFAPQERPLRNLRNYLTSNGLTEVMCMTFSNPRELENAGLAEDVRCQVSL
                     QNPLSENSTGLRTSLIPGLLSTIGSNLRKGSTDLALFETGPVYNLVEGKALPNEFLRC
                     VIALTGAPGGKHWSAPLQKADFYDLKGYFEALMHHLGVSWELVPFESPTYHPRASAQI
                     LVNGKIAGMMGETRPKVLRSFDIHQPVYLLDLDLATLFSVTVQPARFSEPPAFPPSLR
                     DLAIIVDKSVAAAELLQTARTAGGRLLSKVSIFDLYTGKQVPEGKKSVALSLVFQAAD
                     RTLTDADTEKRYEKIIAELRKTCGAELR"
     gene            2131..2427
                     /locus_tag="PKW60_13400"
     CDS             2131..2427
                     /locus_tag="PKW60_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00475.1"
                     /translation="MNPNLQKHFDNLDHRLDALEDLLAAVASRTDKAEEARQQALEEQ
                     LQQRKQLVTLQHKADAYDSVRTDHENLLRIQQEMRERLRKLLALTKALGTEMRP"
     gene            2424..2744
                     /gene="zapA"
                     /locus_tag="PKW60_13405"
     CDS             2424..2744
                     /gene="zapA"
                     /locus_tag="PKW60_13405"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPK00476.1"
                     /translation="MMTPTKHRTTVAGVSLNLPVYGTPEETEELATLVTERIKQIESD
                     STRIDTQAYALSAAVHFAAEASQASKRHQDELDEIATALTALLARLNDIIRRFAISGA
                     VKPQ"
BASE COUNT          620 a          906 c          716 g          512 t
ORIGIN      
        1 caaagtcccc acggccgtcg atggctcgac cctggccggc ggattcaaga tcacccaccg
       61 aaaaatgcgc ggagtcgagt cacaggggat gatgtgtgcc ccggacgaac ttggcatggg
      121 ttcggaccac tccggactcc tcatcctgcc ggccgacgcg cctgtcggcg ccgacgccct
      181 gcatctcctt ggcctggacg acacggtcct cgaaatcgag atcacgccca accggggaga
      241 ctgggccagc atgatcgggg tcgcccgtga attgagcgcg ctgcataaga ctccactgcg
      301 ccagcctcaa ttcaagctcc aggaagacgg ccctcctgca tccggctact cttccgtcac
      361 tattgaggca ccggaactgt gcccccgcta tgcgggccga atcatcacag gcctgaccat
      421 tggcccctca ccgttatggc tccgccagcg cctgcttgcg gcaggccagc ggcccatcaa
      481 caacgtcgtc gacgtgacca accttgtcct gctcgaaacc tgccaagccc tacatgcctt
      541 tgatttcaat aagttacacg agaatcggat tgtggttcgc aacgcgaaac acaatgaaac
      601 aatcgaaaca ctggacgaac agacccggaa attgactccg gagatgctcg ttatcgccga
      661 cgcacggcat cccgttgcgc ttgccggtat tatgggcggc gccgcgtcag aagtgacaga
      721 cagcaccacg gatatcttcc tggaaagcgc ctatttccag cccgctacgg tccgcgctac
      781 cgcccgggca ctcggcatga acaccgaggc ctcccaacgt ttccagcgcg gtgccgaccc
      841 ggaaatggcc atttgggccc tggaccgtgc agcgtacctc attcaactcg tcgctggggg
      901 ggccatcgcg aaaggccgcc tcgacgagta cccgaagcca tttgaaacaa agacgttaca
      961 gctgcgatac gcccggactg accttctcct gggcacggcg gttcctccgg aagagcagcg
     1021 cggcattctc gaacgcctcg cgttcacctc aaccgccact gatgaaacta cttgtaccgt
     1081 acgcatcccc tcgtggcggc atgacgtggg cagggaggcc gacctcatcg aagagatcgc
     1141 ccgggtttac ggctacgaaa aaatcgcggc atcgcttccg ccagttcgcc agaacgagct
     1201 gatttttgcc ccgcaggagc ggcctctccg caatttgcgt aactatctga caagcaacgg
     1261 gttgacggaa gtcatgtgca tgactttctc caacccgcgc gagctggaaa acgccggcct
     1321 ggctgaggac gtccgctgcc aggtgtccct gcagaacccc ttgtccgaga acagcactgg
     1381 gctgcgtacc tcgctgattc ccggcctgct ctccacgatt ggctccaatc ttcgcaaagg
     1441 ctcgacagat ttggccctct ttgaaaccgg tcctgtgtat aaccttgttg aaggcaaggc
     1501 tttacccaat gaattcctcc gttgcgtcat cgcgcttacc ggcgcgcccg gcgggaagca
     1561 ctggtccgca ccgcttcaga aggccgattt ctatgacctc aaagggtact ttgaggctct
     1621 catgcaccac cttggcgtga gttgggaact tgtccccttc gaatctccga cctatcatcc
     1681 gcgagcaagc gcccaaatcc tcgtcaatgg aaaaatagca ggaatgatgg gggagacccg
     1741 ccccaaagta ttacgttcat ttgacatcca tcagcccgtc tacctgctcg accttgacct
     1801 cgctactctg ttttccgtga cagttcagcc agcccgcttt tcagagccac cggctttccc
     1861 gccttcactg agagacctgg cgatcatcgt cgataagtcg gttgcggccg ccgaactact
     1921 ccagactgct cgcacggccg gcggccgcct gctctcgaaa gtctcgattt tcgacctgta
     1981 taccgggaaa caggtgcccg aaggcaagaa gagcgttgcc ctcagtctgg ttttccaggc
     2041 ggcagaccgc acacttacag atgccgatac cgagaaacgc tatgagaaaa tcattgccga
     2101 gttgagaaaa acctgcggcg cggagctccg gtgaatccta acttacaaaa gcacttcgat
     2161 aacctcgacc acaggcttga cgccctggaa gatctcctgg ccgcggtcgc ttcccgcacc
     2221 gacaaagccg aagaggcgcg ccaacaggct ctagaggagc aactccaaca acgtaaacaa
     2281 cttgtaactc tgcaacacaa ggcagacgcc tacgacagcg tccgaacgga tcacgaaaac
     2341 ctgcttcgta tccagcagga gatgcgcgaa cgcctccgaa agctgcttgc cctcacaaag
     2401 gcccttggaa cggagatgcg gccatgatga ccccgaccaa gcaccgaacc accgtagccg
     2461 gagtctcatt gaatctgccc gtctacggaa cgcctgaaga gacggaggaa ctcgcgaccc
     2521 tcgtaaccga acgcatcaaa cagattgaat ccgattcaac tcgcatagac acacaggctt
     2581 acgcactttc cgctgccgtg catttcgccg cagaggccag ccaggccagc aaacgccatc
     2641 aggacgaact cgacgaaatc gccaccgcac tgacggcctt gctcgcgcgc ctgaacgaca
     2701 tcatccgccg attcgccatc tccggcgccg taaaacctca ataagctcaa gggt
//
LOCUS       DAOPBQ010000500         2745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_29069_length_2745_cov_4.547212, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000500 DAOPBQ010000000
VERSION     DAOPBQ010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29069_length_2745_cov_4.547212"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="PKW60_13410"
     CDS             complement(<1..1383)
                     /locus_tag="PKW60_13410"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00477.1"
                     /translation="MAGKKEVSRRAFMRDAARTAAGITAGAATVATARADVYKSILPS
                     SVLGANEMIRTGHIGLGVMGSGNLKYNMQRPDVMPIAVCDLHPRNRGKGHAMAQAKNP
                     QATVHEDYKEIIDNPDVDAVICSTTDHWHALVSIAACDAGKAVYCEKPLGTTVKEGQA
                     MVAAAQRNNTVFQCGTIQRSGEHFKEAIEIVKSGTLGQIGHVHTFSIDMLAPTGIGNP
                     PDVTDPEKWKSYGPWDTYQGWVEHKPFNVNRWLYNFRWFYDYAGGKLTDWGVHLVDIV
                     VSAMGEDKPPKTVSASGGKFIITDNRTTPDTINVSWEFDDYLLTFTNRVWNSYGDQIA
                     PADNHGIIFYGTKGSLKLSRAGYKLIPGANQQYVPEPGEYESAEARENNSASVLYEPH
                     LENFIACIRSGEKPISHVESCHNSSTICHIGNAAYLAGAKLTWDAAAEQFTGGPADAV
                     KQANEWIARPY"
     gene            1751..>2745
                     /locus_tag="PKW60_13415"
     CDS             1751..>2745
                     /locus_tag="PKW60_13415"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPK00478.1"
                     /translation="MKHERRVVVTGLGAITPLGVGLEPTWEGLLAGKNGIDRLTHFDP
                     SQYRSQIAGEVRDFRVEDWIDGKSARRMDRVMHFGLAVSKMALADAALDSASYDKNRA
                     GVIIGSGIGGSASIEEGCAASREKGPRAISPFFVSKALINMTACAISIEHGLKGPLTA
                     LSVACSTGANAVGDAFRVIQRGDADIMVAGASEACLTPAPYGGFCATRSMSTRNDDPT
                     RASRPFDRNRDGFVMGEGAGVAVLEELEHARARGARIYAELAGYGNTADAYHFTAPEP
                     EGDGMLRVMRAALNDAGLAPEAVGYINAHGTSTVLNDRTESLAIERVFGAHAKKL"
BASE COUNT          452 a          841 c          874 g          578 t
ORIGIN      
        1 gtacgggcgc gcaatccact cgttggcctg tttgaccgca tcggccggtc caccggtgaa
       61 ctgctccgcc gcggcgtccc acgtcaattt ggcgcccgcg aggtacgctg cgttgccgat
      121 gtggcagatc gtgctcgaat tgtggcatga ctccacatgg ctgatgggtt tttcgcccga
      181 tcggatgcag gcgatgaagt tttcgaggtg cggctcgtag agcaccgacg cgctgttgtt
      241 ttcccgggct tcggcggatt catattcgcc gggttcgggc acgtactgct gattcgcgcc
      301 cggaatcagc ttgtatcctg cgcggctgag tttcaacgac cccttggtgc cgtagaagat
      361 gatcccgtgg ttgtccgccg gcgcgatctg gtcgccgtag gaattccaga cgcggttcgt
      421 gaaggtcagc aggtagtcgt caaattccca gcttacgttg atggtgtccg gcgtggtgcg
      481 gttgtcggta atgatgaatt tgccgcccga cgccgagacg gtcttgggcg gtttatcttc
      541 gcccattgcc gacaccacga tgtcgaccag gtgcacgccc cagtcggtca gtttgccgcc
      601 cgcataatcg tagaaccagc ggaaattgta gagccagcgg ttcacgttga agggtttgtg
      661 ctccacccag ccctggtacg tatcccacgg cccgtacgat ttccatttct cgggatccgt
      721 cacgtccggc ggattgccga tgccggtcgg cgccagcatg tcaatcgaga acgtgtgcac
      781 gtggccgatc tggccgagcg ttccgctctt gacgatctcg atggcctcct tgaagtgttc
      841 gccgctgcgc tgaatggttc cgcactggaa caccgtgttg ttgcgctgtg cggccgcgac
      901 catcgcctgg ccttctttca ccgtggtgcc cagcggcttc tcgcaataca cggccttgcc
      961 cgcatcgcag gcggcgatcg agaccagcgc gtgccaatgg tccgttgtcg agcagatcac
     1021 cgcgtccacg tccgggttat cgatgatctc tttgtagtct tcatgcaccg tcgcctgcgg
     1081 gtttttcgcc tgggccatgg catgtccctt gccgcggttc cgcgggtgca ggtcgcacac
     1141 cgcgatcggc atgacatccg ggcgctgcat gttgtatttc aggttgcccg agcccataac
     1201 tcccaagccg atatggccgg tgcgaatcat ctcgttggcc ccgagcacgc tcgagggaag
     1261 aatcgattta tacacgtccg cgcgagccgt tgcgaccgtc gctgcgcccg ctgtgatgcc
     1321 cgccgctgtt ctggccgcat cgcgcataaa tgcccggcgg ctcacttcct tcttgcctgc
     1381 catcgtgtac ctccattatc tgactacgcg gtcgctgata cgcgccgggt tgctccgatt
     1441 cgccgcctgg cggaccggcc tcaaggattg cggtcatggt actccaaacg aggccgcgtt
     1501 tccagaatcc gccccgccgc atgaagcagg gccctgacat gcagttgccg cgggcggcac
     1561 gatcgccggc tccggccgat actgcgaaca ggcccacgat tgctctgcat cacgctcttg
     1621 aacttcatcc gtgccgcccg taccatggcg cttgcgtttc aaccaggcag ggaggaatgg
     1681 ctgcgagcgc atgtctaccg tttgcggcgg cgcggcggct ggcgagcgtt gcatccaaga
     1741 ggattgcatt ttgaaacacg aacgccgcgt tgttgtcacg gggctcggcg ccattacacc
     1801 gcttggtgtt ggtctggagc ccacgtggga ggggcttttg gcggggaaaa acggcatcga
     1861 ccgcctcacc cattttgatc cctcgcagta ccgctcccag attgccggcg aagtgcgcga
     1921 ttttcgggtg gaagactgga tcgacggcaa atctgcgcgc cggatggatc gggtgatgca
     1981 ttttggcctc gctgtctcga aaatggccct tgcagatgcc gccttggaca gcgcctcgta
     2041 cgacaagaac cgcgctggcg tcatcatcgg ttcgggcatt ggcggttcgg cgagcattga
     2101 agaaggctgc gcggcatcca gagagaaggg gccacgcgcc atcagcccgt tttttgtgtc
     2161 gaaggcgctg atcaacatga ccgcgtgcgc gatctccatc gagcacggac tgaaaggccc
     2221 gctgacggcg ctgtcggtgg cctgttcgac cggcgccaat gccgtgggcg acgcattccg
     2281 tgtgattcag cgcggagacg cggacattat ggtggcaggg gcctcggaag cctgccttac
     2341 cccggcgccg tacggagggt tctgtgcgac gcgttcgatg tcgacccgga acgacgaccc
     2401 cacccgcgcc agccggcctt tcgacaggaa ccgcgacggg tttgtgatgg gagaaggcgc
     2461 gggcgtggcc gttctggagg aattggagca tgcccgggcc cgtggcgcac ggatttacgc
     2521 ggaattggcg ggctacggga atacggccga cgcataccat tttacggctc cggagccgga
     2581 aggcgacggc atgctgcggg tgatgcgcgc ggcgcttaat gatgcgggtc tcgctcccga
     2641 ggccgtcggc tacataaatg cccacggcac gtcgacggtc ctgaacgacc gcaccgaatc
     2701 cctggccatc gaacgggtgt tcggcgcaca tgcgaaaaag ttgaa
//
LOCUS       DAOPBQ010000501         2745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_29076_length_2745_cov_3.427881, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000501 DAOPBQ010000000
VERSION     DAOPBQ010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29076_length_2745_cov_3.427881"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1200)
                     /locus_tag="PKW60_13420"
     CDS             complement(199..1200)
                     /locus_tag="PKW60_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPK00479.1"
                     /translation="MKNQPLSIAHYIKRFFAEYLPSHRNCSPHTIASYSVALRLFLVY
                     LRERKKIAPAKLAVRDLSAGNIMEFLNEIQASRRNGTGTRNVRLAAIRSFVNYLLLLE
                     PALTSELQAVLAIPVKRDTRRVLDFLTREELDAVLAAPQEATWSGKRDRILYTVMYNS
                     GARVSEIVAAKVSDVKLNPGATLRLHGKGRKDRVLPLWKSTAKNLGQWIAGNRLEADA
                     PLFANARGAHMTRSGVEKRLREAVRAAREKCPSLKSKRVSPHTLRHTTAMHLLHSGVD
                     ITLIAMWLGHESIETTHIYMTTDLAMKEKALQALQSPTPGKFRFQPNDELIAFLESL"
     gene            complement(1197..2174)
                     /locus_tag="PKW60_13425"
     CDS             complement(1197..2174)
                     /locus_tag="PKW60_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPK00480.1"
                     /translation="MTKKTPSMLEMVNNYVEHKRGLGYKLGTESYMLRSFARHADRQA
                     PGQPLTTQLALEWATAPESDGRTYHAKRLDALRSFARYLAAFQPGTEIPPGGILGPSL
                     SRVEPHIYTREEVAALMREAMNVKPSTPAARTNSIRNATVIGLLACTGMRIGEVLALK
                     NEDVDLATGVITVRESKKLPMRLVPITDCAVRQLRRYQQERDRRFGSPSPSGAFIQSS
                     WNGRLTYGSFFAAFKIIRERAGVKRSNVPNRPPRLHDLRHTFACNHLLRAYREGRDID
                     NAVHELAVYLGHATLKSTYWYLTGVPALFEQCAKRFEAQAARDVKGGRT"
     gene            complement(2171..>2745)
                     /locus_tag="PKW60_13430"
     CDS             complement(2171..>2745)
                     /locus_tag="PKW60_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPK00481.1"
                     /translation="PKVLEASDLKKLLASIDRSTPTGKRDYAAVLCMCDLGLRVGDLP
                     RLTLDDIDWRRGSVQVANHKMDRPYRLPLPRRVGRALADYLAHGRPASACRQIFVRHG
                     RPIGQPTTAHALKAAVYRLWDKSGQQDRFSGTHILRHSAATRMKRAGVTLKSIADVLG
                     HGSMQTTVLYAQVDLPALRAAAQPWPEVQP"
BASE COUNT          479 a          815 c          908 g          543 t
ORIGIN      
        1 actggatcgc ctgggcgacc cgctcgcgcg tccgtttgac cgtctcgatc tcgatcttat
       61 gcggagttcc gcataagatc gattatgtgg agttcccggt aatgcggagt tgattgagag
      121 aggcggtccc ggatgcccgt gctggggatc aaggacacgc ctggccttac gttttactcc
      181 gcattattcg gcgcttcatt acagggattc caggaacgcg atgagttcgt cgttgggttg
      241 gaaacggaac ttgccgggcg tcggcgattg caatgcctgg agcgcctttt ccttcatcgc
      301 caggtcggtg gtcatgtaga tgtgcgtcgt ctcgatgctc tcgtgaccca gccacatggc
      361 gatcagagtg atgtccaccc cggaatgcag caggtgcatc gctgtcgtgt ggcgcagtgt
      421 gtgtggcgag acacgtttgc ttttgagtga ggggcatttc tcccgcgccg cccgtacggc
      481 ttcgcgcagc cgtttctcca cgcccgaacg cgtcatgtgg gcgccccgcg cgttggcgaa
      541 cagcggagcg tcggcctcga gccggttgcc tgcaatccat tggcccaggt tcttggccgt
      601 gcttttccat agcggcaaca cccgatcctt gcggcccttg ccatgaaggc ggagggtcgc
      661 gcccggattg agtttgacgt ccgacacctt cgcggcaaca atctccgaaa cacgcgcgcc
      721 gctgttgtac atcacggtgt ataggatgcg gtcgcgcttg ccgctccatg tggcttcctg
      781 cggcgcggcc aggacggcgt ccagttcttc gcgcgtcagg aagtcgagga cgcgccgcgt
      841 gtcgcgctta acggggatcg ccagcacggc ctgcagctca ctggtcagcg caggctccag
      901 cagcaacaga tagttgacga acgagcgaat ggccgcgaga cggacgttgc gggtgccggt
      961 cccgttcctg cgcgaggctt ggatttcatt gagaaactcc atgatgttgc cggccgaaag
     1021 gtcccgcacg gccagtttgg ccggggcgat cttcttgcgc tcacgcagat acaccaggaa
     1081 caggcgcaac gcgacgctgt aagaagcgat cgtatgaggg ctgcaatttc ggtggctggg
     1141 caaatactcc gcgaagaaac gcttgatgta atgcgcgatg ctgagcggct ggttcttcat
     1201 gtccgacctc ccttgacgtc gcgagcggcc tgggcttcaa agcgcttggc gcattgttcg
     1261 aacaaggccg gaacgccggt cagataccag taggtggact tcagcgtcgc atgccccaga
     1321 tagactgcca gttcatgcac ggcgttgtcg atatcgcgcc cttcccggta ggcgcgcaga
     1381 agatgattgc aggcaaaggt gtggcgtagg tcgtgaaggc gcggcggacg attcgggacg
     1441 tttgagcgct tcaccccggc tcgttcacgg atgatcttga aagcggcaaa gaacgagccg
     1501 tacgtcaacc gtccattcca ggatgattgg atgaacgctc ccgacggcga tggcgagcca
     1561 aagcgccggt cgcgctcctg ctggtagcgg cgcagctgac gcacggcgca gtccgtgatc
     1621 ggaaccaggc gcattggcag cttcttgctc tcacggactg tgatcacgcc cgtggccaga
     1681 tccacatcct cattcttcaa cgccagaacc tcaccaatcc gcatccccgt gcaggccagc
     1741 agtccgatca cggtagcatt gcggatggag ttggtccggg cggccggggt ggacggcttc
     1801 acattcatgg cctcgcgcat cagcgcggca acctcctcac gggtgtagat gtggggctcc
     1861 acgcgcgaaa gactgggacc aagaatccct ccgggcggga tttcggttcc gggctggaac
     1921 gccgccagat aacgggcgaa cgaacgcagc gcgtccagac gcttggcgtg gtaggttctg
     1981 ccgtccgatt cgggtgcggt tgcccattcc agggccagct gggtggtcag cggctggccg
     2041 ggggcctgcc ggtcggcgtg gcgggcgaag gagcgcaaca tgtagctctc ggtgcccagc
     2101 ttgtagccca gcccgcgttt gtgctcgacg taattgttaa ccatttccag catcgatggc
     2161 gtcttcttgg tcatggctgc acctccggcc agggctgcgc cgccgcgcgc agggcgggaa
     2221 gatcgacttg ggcgtagagc acggtagtct gcatcgagcc atgacccagc acatcggcga
     2281 ttgacttaag cgtgacgccc gcgcgtttca tgcgcgtggc ggcgctgtga cgcaggatat
     2341 gggtgccgga gaatcggtct tgctgaccgg atttgtccca gaggcgatag acggcggctt
     2401 tgagggcgtg ggccgtagtg ggttggccga tcgggcgccc atggcgcaca aagatttgcc
     2461 gacacgcgga ggccgggcgg ccatgggcca ggtagtcggc caatgcccgt cccacacgcc
     2521 ggggcagggg cagacggtag ggacgatcca tcttgtgatt ggcaacttgc accgaacccc
     2581 ggcgccaatc gatgtcgtcc agcgttaatc gcggcagatc gccgacgcgc aggcccagat
     2641 cgcacatgca caggaccgcc gcgtagtcgc gcttgcccgt cggcgtcgag cggtcgatgc
     2701 tggcgagcag ttttttcaag tccgacgcct ccagcacctt gggag
//
LOCUS       DAOPBQ010000502         2741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_29145_length_2741_cov_2.704393, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000502 DAOPBQ010000000
VERSION     DAOPBQ010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29145_length_2741_cov_2.704393"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PKW60_13435"
     CDS             <1..852
                     /locus_tag="PKW60_13435"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPK00482.1"
                     /translation="EHIAGLTSVIDSTLNKELVRDLVREADVVYHLAATVGVQLVVEQ
                     PVRTLVNNIRGTENILEETCRYRRRLLITSSSEVYGKGISPQFQEDDDRVMGATSLSR
                     WGYAASKAMDEFLAFAYWREKRHPVVIVRLFNTVGSRQTGRYGMVIPRFVAQGLRNEP
                     LTVYGDGEQSRCFTYVGDIVPALIRLMERVDISGEVFNIGGIQPITMKELAQRVVQKT
                     GSTGGVTFIPYDQAYGPGFEDMRARVPNIRKIRDAVGFEPRTSLDEILDVVIGDVRRR
                     LRDQSPI"
     gene            complement(947..1417)
                     /locus_tag="PKW60_13440"
     CDS             complement(947..1417)
                     /locus_tag="PKW60_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00483.1"
                     /translation="MPDRFTVFRARVVEQASISNPATAYEETGLGTLAVLALSSPIYA
                     EPIDLTPPGAAPPEPAEAPAPALASIPVEERVKKAMEAVERIAEGKEEKNWISIVPEG
                     LPPIYATGVRTQGGKVSVTTDHSRPPQFFQEILDEEFLPSPKAKIQEIQPRMIR"
     gene            complement(1436..>2741)
                     /locus_tag="PKW60_13445"
     CDS             complement(1436..>2741)
                     /locus_tag="PKW60_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00484.1"
                     /translation="RNKNVDQAYFLKVNDGTAVADCPPGEYTAYIYVYDRGVPVLVDI
                     HDITVEINGMASIPLTLTEGSGQLPLQSFDADLDLVLDRVEEEQGTDRFDAASMPGTT
                     PLPDRNVVLKNSAGWYKGELHAHSSYGEGTETVASLVRGAEKARLDFLAITDLNTMAA
                     CQDPGFTSSSVVLIPAMKWGDTKRGYALVYGPRTIPERADTFAHAQAIMRRVQSQGGI
                     FAIAHPCFRDAPWQWGLEYANAIEVWCRDWRSMPPVQLTDLDEDLQVRGEKRLDRDPK
                     TALKVPGKYVEGRLIYPIAQAAATTHLAANAKSEIFWDAHLNSGMRVSPIAGSMASAT
                     AVSLGQPITYVYASEKSLSGILRGLRQGRTFVSAGPNAPTVDLYANVKLNNRTIFVPM
                     GGMIPIGTEARLVVSVRNGKGKKVQIFADGQPIISKTMTSKQ"
BASE COUNT          517 a          856 c          810 g          558 t
ORIGIN      
        1 gagcacatcg ccggcctcac cagcgtcatc gattccacgc tcaacaagga gctggtgcgc
       61 gacctggttc gcgaagccga cgtggtctat catcttgccg ccaccgtagg cgtgcaactc
      121 gtcgtcgagc agccggtccg aacgctcgtc aacaatatcc gggggacgga gaacatcctc
      181 gaagaaacgt gccggtaccg ccggcggctt ctgatcacct ccagcagcga ggtgtacggc
      241 aaaggcataa gcccccagtt ccaggaagac gatgaccgcg tcatgggcgc aacatcgctg
      301 tcccggtggg gatatgccgc gtcaaaagcc atggatgaat tcctcgcctt tgcctactgg
      361 cgcgaaaaac gccatcccgt ggtgatcgtg cgccttttca acaccgtagg atcccgccag
      421 acgggccgct acggcatggt catcccgcgt tttgtagccc aggggctgcg gaatgagccg
      481 ctcaccgtat atggagacgg tgaacagtcc cgctgcttca cctacgtcgg cgatatcgtg
      541 ccggcgctga tccggctgat ggagcgggta gatatatcag gcgaagtctt caatattggc
      601 gggattcaac ccattaccat gaaagaactg gcgcagcgcg tggtccagaa gaccggcagc
      661 acaggcggcg tcacattcat cccgtatgac caggcctacg ggcccggttt cgaagacatg
      721 cgcgcgcgcg tccccaatat ccgcaagata cgcgacgctg tgggattcga gccccgtacc
      781 tcactcgatg aaattctcga tgtggtcatt ggcgacgtgc ggcgccggct tcgcgatcaa
      841 agccccattt gaccacgcca attcggccgt tccccgctgg ccggcagagt gagaaccggg
      901 gaaatcatgc cgctgccgac gcaatcctcc gccgatggcg gttgcattac cggatcatcc
      961 tgggctggat ttcctggatt tttgccttgg gtgacggcag gaactcttca tccagtattt
     1021 cctggaagaa ctgcggcggg cgcgaatgat ccgtggtcac ggaaacctta ccgccctggg
     1081 tccgcactcc cgtcgcgtag atgggcggca atccttccgg cacgatgctg atccagtttt
     1141 tctcctcttt cccttcggca atgcgctcca cggcctccat agccttcttg actcgttcct
     1201 ccacgggaat cgaggccaga gccggggccg gagcttccgc gggctccgga ggggctgccc
     1261 cgggcggcgt cagatcgatg ggctccgcat agatgggact cgagagagcc agaaccgcga
     1321 gtgtgcccaa ccccgtctct tcgtaggcgg tcgcgggatt ggagatagag gcctgctcca
     1381 cgacgcgcgc ccgaaacacc gtaaagcggt ccggcatgat gtcgatacgc cggtcttact
     1441 gtttggaagt catggtcttg gaaatgatgg gctgcccatc cgcgaaaatc tgaacctttt
     1501 tgcccttgcc gttgcggacg ctgacgacca gccgggcttc cgtcccgatg ggtatcatgc
     1561 cgcccatggg cacaaagatg gtgcggttgt tgagcttcac gttcgcgtac agatcgacgg
     1621 tgggagcatt cgggcctgcc gatacaaagg ttcgaccctg gcgaagccct cggaggatgc
     1681 cgctcaggga tttttcgctg gcatacacgt aggtaatcgg ctgccccagc gataccgcag
     1741 tggccgatgc catgcttccg gcaatgggac tcacgcgcat gccgctgttc aggtgcgcat
     1801 cccagaaaat ctctgacttg gcattcgcgg ccaggtgggt ggtcgcagcc gcctgggcta
     1861 tcggatagat cagccgccct tcgacgtact ttccgggcac cttcagggcg gttttcggat
     1921 cccggtccag gcgtttctca ccgcgaacct gcaagtcctc gtccagatcc gtcagctgca
     1981 cggggggcat gctgcgccaa tcccggcacc acacttcgat ggcgttggcg tactccagcc
     2041 cccactgcca cggcgcatcg cgaaaacacg gatgagcgat cgcgaagatc ccgccctggc
     2101 tctgtacccg ccgcatgatc gcctgggcat gcgcgaacgt gtcggcacgt tccggaatgg
     2161 tccggggtcc gtagacgagc gcataccctc gcttggtgtc accccatttc atggccggaa
     2221 tcaagacgac cgacgacgat gtaaagcccg gatcctggca cgccgccatg gtgttgagat
     2281 cggtgatcgc cagaaaatcc agacgagcct tttcggcacc ccgcacgagg ctcgccacag
     2341 tctccgtgcc ttctccgtaa ctcgaatggg cgtgcagttc gcctttatac cagcctgcgc
     2401 tgttcttcag gacaacattt cgatccggca ggggggtcgt tccgggcatg cttgccgcat
     2461 caaaccgatc ggtcccctgc tcttcttcga cccggtcgag caccagatcc aggtcggcat
     2521 cgaacgattg cagcggcagc tggccgctgc cttccgtcag cgtcaacggt atgctggcca
     2581 ttccgttgat ttcgacggtg atgtcatgaa tgtcgaccag gacaggtact ccgcggtcgt
     2641 aaacatagat gtaggcggta tattctccgg gtgggcaatc ggctacggcc gtaccatcgt
     2701 tgactttcag gaagtaggcc tgatccacat ttttattccg c
//
LOCUS       DAOPBQ010000503         2736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_29216_length_2736_cov_4.390899, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000503 DAOPBQ010000000
VERSION     DAOPBQ010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29216_length_2736_cov_4.390899"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1346
                     /locus_tag="PKW60_13450"
     CDS             171..1346
                     /locus_tag="PKW60_13450"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPK00485.1"
                     /translation="MYRLFLQSYWRLARLVFYSLFRSRGTLGELSVRRFVLLCAAVPL
                     LLILNSMHWMCFLADEILFSAYRKVKVSQPLFIVGVPRSGTTFFHRLLSGDSGRFTTM
                     TLGEILFAPSILQRRIFQGLGRVDAALGGFGRAAVEALDRRFFRSLHHLHPQSLFQPD
                     EDEQVLFPIIHSASLALLFPFEIEMAYLMRFDEAAGPELRRDVMCFYRRMVQRHLYFH
                     GPDKQYLSKSPAFSVKLRSIRETFPDARVVCNVRSPFECIPSMASLGKFYWDTFGNHL
                     YEEEFRRQFLKIGHCFYQGPMRVLPEWPEDQYAALLYPELVGHPRASVEAIYARFGWP
                     VSDDFRDYLAGQECRGRTYKSSHAYSMEEYGISRALLLDQMRDVFDYYGFPTHPDAA"
     gene            complement(1427..>2736)
                     /locus_tag="PKW60_13455"
     CDS             complement(1427..>2736)
                     /locus_tag="PKW60_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPK00486.1"
                     /translation="HVPAPEARVDAPFLMQVSTLGWRDHIGRTGTGRVLQGRLQRGDA
                     LTRITTRRTRAEWLRQKADDGDDWEVTGIEDDRAVHLWVTRGIESVPCSEVSAGDIVT
                     IAGPKAISIGDTLAAPELDNCALPPLVIEEPTVAMTIQVNTGPFAGRDGAAVTIRQIR
                     ARLDRELRTNVALRVEETGRGNGMKVSGRGELHLAILIEEMRREGMELCISPPEIITH
                     CDAAGTLFEPFEELVIDVPLEFQGTVIEKISLRKGQLQRVETNGAGMVRLEFAITTRG
                     LIGYRSELLTDTRGLGIMASRFTGYERWTGPIITRNRGSMISMDTGEATSYSLENLQQ
                     RGVLFVSPMDPVYEGQIVGEHARPGDLPCNPTKRKALTNHRAAGKDHAIGLDVPRTLT
                     LDAALEWVATDELVEVTPKVIRVRKAVLNAEDRKRLHKRTADE"
BASE COUNT          461 a          769 c          881 g          625 t
ORIGIN      
        1 caggggcgag acaaccctgc ccgggagcgc cccttcggcc acggccgcaa cctgcgtgct
       61 atgtaaaaga ttcaattacc tttctctttt gatgggccgg cacgcgaatc tcgaaggccg
      121 agcagtaacg ttccgcgtac tggcgtgtag aaaccctgca agaggcgtgc atgtatcgat
      181 tatttctgca gagttattgg cgtttagcgc ggctggtgtt ctattcgttg ttcagaagcc
      241 gtgggactct gggcgaattg agcgtccggc gctttgtgct gctgtgtgcc gccgtgccct
      301 tgcttttaat tctgaactcc atgcactgga tgtgctttct ggccgacgag atattgtttt
      361 cggcgtaccg gaaggtgaag gtatcacaac cactgtttat tgtgggcgtg ccgcgcagcg
      421 gaacgacgtt ttttcaccgc ctgttgagtg gcgacagcgg ccgcttcacc acgatgaccc
      481 tgggggagat tctcttcgcg ccgtcgatac ttcagcgccg catttttcag gggctgggac
      541 gagtggatgc tgcgttaggc gggttcggcc gtgccgcggt ggaggccctt gaccggcgtt
      601 tcttccgaag cctgcatcat cttcatccac aaagcctttt ccagcctgat gaagacgagc
      661 aagtgctgtt tccgatcatc cactcggcct cgctggctct tttgtttcca tttgaaatcg
      721 agatggccta cctgatgcgt tttgacgagg ccgccgggcc ggagcttcgg cgcgacgtga
      781 tgtgtttcta ccgcaggatg gtgcagcgcc atctctattt tcacggtccg gacaagcagt
      841 atctctcgaa gagccccgcg ttttcagtaa aactgcgttc catccgggag acgttccctg
      901 atgcccgggt tgtatgcaac gtccgctctc cgtttgaatg catcccttcg atggccagcc
      961 tgggaaaatt ctactgggac acattcggca accacctgta tgaggaggaa ttccggcggc
     1021 agtttctcaa gattggccac tgcttttatc aggggcccat gcgtgttttg ccggaatggc
     1081 cggaagatca gtatgccgcg ttgctgtatc ccgaactggt ggggcatccg cgggccagtg
     1141 tggaagcgat ttacgcaagg ttcggatggc ccgtgagtga tgactttaga gactatctgg
     1201 caggacagga atgcagaggc cggacgtaca aaagttcaca cgcctattcc atggaggaat
     1261 atggcatcag ccgggccttg ctgctcgatc agatgcgcga cgtcttcgac tattatggat
     1321 ttccgaccca cccggatgcc gcctgagcgc gagagcggcc ggcgcgcgaa cacattcgca
     1381 ctatccgttg ccggtatggc atcgttgggt tccgttgccg tgagagtcac tcgtccgcgg
     1441 tacgtttatg aaggcgcttg cgatcttccg cgttcaagac cgctttccgg acgcgaatga
     1501 ccttcggggt gacttccacc agctcgtcgg tggcaaccca ttcgagagcg gcatcgagcg
     1561 tgagggtgcg ggggacatcg agtccgatgg cgtggtcctt gccagcggcg cgatggttgg
     1621 tgagcgcctt gcgtttggtc ggattgcacg ggaggtcgcc cgggcgcgcg tgttccccga
     1681 cgatctgccc ttcgtacacg ggatccatgg gcgagacaaa aagcacgccg cgctgctgaa
     1741 ggttttcgag gctgtatgag gtcgcttcac cggtgtccat gctgatcatg gagccccggt
     1801 tgcgggtaat gatggggccg gtccagcgtt cgtatcccgt aaagcgcgag gccatgatgc
     1861 caaggccgcg tgtgtcggtg agcaattcgc ttcggtatcc gatgaggccg cgtgtggtga
     1921 tggcaaattc aagacggacc atgccggccc cgttagtttc gacgcgttgg agctgtcctt
     1981 tgcgcaggga tattttttca atcaccgtgc cttgaaattc cagaggcacg tcgatgacga
     2041 gttcttcgaa cggctcgaag agcgtccccg cggcgtcaca gtgcgtgatg atttccggcg
     2101 gggaaatgca gagttccatg ccttcgcggc gcatttcctc gatgaggatg gcaagatgca
     2161 gctcaccgcg gccggagacc ttcatgccgt tgccccggcc ggtttcttcg acgcggagcg
     2221 ccacgttggt ccgcagttcg cggtccaggc gggcgcggat ctggcggatg gtgacagcag
     2281 cgccgtcgcg gcccgcgaat ggtcccgtgt tgacctggat cgtcatggca acggtgggct
     2341 cttcgatgac gagcggcggg agggcgcaat tgtcgagttc aggtgcggca agcgtgtcgc
     2401 cgatgctgat ggccttcggg ccggcaatgg tcacgatatc gcctgccgaa acttcgctgc
     2461 atggcacgct ttcaatgccg cgcgtgaccc agagatgcac ggcgcggtcg tcttcgatgc
     2521 ccgtcacttc ccagtcgtct ccgtcgtcgg ctttttgccg gagccattcc gcacgggtgc
     2581 gtcgcgtggt gattcgggtc agggcgtcgc cgcgctggag gcgtccctgg aggacgcgcc
     2641 ccgtgccggt gcggccgatg tggtcgcgcc agccgagcgt gctgacctgc atgaggaacg
     2701 gcgcatccac gcgggcctcg ggggcgggta catgcg
//
LOCUS       DAOPBQ010000504         2734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_29240_length_2734_cov_3.193729, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000504 DAOPBQ010000000
VERSION     DAOPBQ010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29240_length_2734_cov_3.193729"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PKW60_13460"
     CDS             complement(<1..970)
                     /locus_tag="PKW60_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HPK00487.1"
                     /translation="MIFILFALLPGRRAALAGFLGGWMFLPNAGFQIQYFPEYNKAMA
                     ICVGIFAATLVFDMDRFRRFRPCWIDLFPLTLSIVPFFSSISNGLGAYDGFSAMTGAF
                     ASWVLPYLVGRLYFSDLQGMRELILGFFIAGLVYLPFCLWEMRMSPNLHYHIYGFNPS
                     AFVQAYRGGGYRPVVFMRHGLMTVMFMGMSALCGCALWLSGAKKTLLKLPIAVPVGAL
                     AVTTLLSRSTGAQVLMLAGIGALFFMRYARNPLPVVATALFVIAYVVLRGGGLWDGQD
                     LIRLADDAFGSERASSLETRIANENALAAKARQRPLLGWGGWGRNRV"
     gene            complement(1157..2638)
                     /locus_tag="PKW60_13465"
     CDS             complement(1157..2638)
                     /locus_tag="PKW60_13465"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPK00488.1"
                     /translation="MPADDPASGPSHDAEAAGAPQARPRSLKRLTIRGSVWTFAGYGI
                     TQTIRLGGNIVLTRLLFREAFGLMVMINIYLQGLAMLTDLGIAPSIVQNPKAEDPEFL
                     RTAWTLQLLRGLGLWLGALAFTLPAAWYFNQPALRYFLPVAGLNLLIVSLDSTNLHLL
                     HRHVNVPLQIQLSVITQVAGLMVSMAGALAWRIYAEVRTLNAMFVTFSAGGDWRQIQF
                     DDSFYWMGVWALVVGNLAAMVFHTLLTYQIPGPRMGFAWRKEYAHQLIHFGKWIFVSS
                     LLTFLANNLDRTVLARFLDVGMLGVYGIAYMFASSIVDLLRSLSNRVLFPVYAEMARE
                     RPGALRRHSTRIRLLLMSVTLPVLWGMVLAGPEFIRIVYQPEYWSAGRLLQMLACGSI
                     LSGMAAPVEVVFLAVGDSFRHFLTMLSRSAMLLLAMGIGGWLNGSDGIIWGIVLAPAL
                     HYPFLSLLGRKYGVWVPWLDALGVAASAIMLTAGLWCKHLILG"
BASE COUNT          635 a          832 c          860 g          407 t
ORIGIN      
        1 tgacgcggtt tcgtccccag ccgccccagc cgagcaaagg ccgctggcgc gccttggcgg
       61 cgagcgcgtt ttcgttggcg atacgcgttt cgagcgagct ggcgcgctcc gagccaaacg
      121 catcatcagc gaggcggatc aggtcctggc cgtcccacag gccgccgccc cggagcacca
      181 cataggcgat gacgaacagc gcggtggcga cgacgggaag gggatttctg gcgtagcgca
      241 tgaagaacag cgcgccgatg ccggccagca tcaagacctg ggcgcccgtg gatcggctca
      301 gcagagtcgt cactgccagg gcgccgacgg gcaccgcgat gggcagcttc aggagcgtct
      361 ttttcgcacc ggacagccac agggcgcagc cgcaaagcgc gctcatgccc atgaacatga
      421 cggtcatgag tccatgccgc atgaaaacca cgggccggta gccgccgccc cggtacgcct
      481 gaacgaaggc gctcggattg aatccgtaga tgtggtaatg aaggttgggg ctcatgcgca
      541 tctcccagag gcagaacggc agatagacaa gccctgcgat gaaaaacccc aggatcaact
      601 cgcgcattcc ctggagatcg ctgaaataca gcctgcccac gagatacgga aggacccagg
      661 aggcaaaggc gcctgtcatg gcggaaaaac cgtcgtaggc cccaaggccg ttcgagatgg
      721 acgagaaaaa cggcacgatg gacagagtca gcgggaacag gtctatccag cacgggcgaa
      781 accgacggaa tcgatccata tcgaagacca gcgtggccgc aaaaatacca acgcagatgg
      841 ccatcgcttt gttgtattcg gggaaatact ggatctgaaa cccggcattg ggaagaaaca
      901 tccatccgcc caaaaagcct gccagcgcgg cccgccgtcc gggcaacagg gcaaaaagga
      961 tgaatatcac cggaatgcat cccagcagta cgacggggac cagactgttg ccctgaggca
     1021 tgaccagcat tgcgatgccc tttgccgtta atcggatggc ccatcagcgg gcgagccgtt
     1081 ctctccaaac cccattgtag gggcctggcc gcaattcgcg caaaaacacg gagcgccggc
     1141 agtactcagc gcctgttcac cccagaataa gatgcttgca ccagagtccc gccgtaagca
     1201 tgatggccga ggccgccacg cccagcgcat ccagccaggg cacccatacc ccgtacttgc
     1261 ggcccaggag cgacaggaag ggataatgca gcgcaggcgc gagcactatg ccccagatga
     1321 tgccgtcgct gccgttcagc cagccgccaa tgcccatggc caggagaagc atcgctgaac
     1381 ggctcaacat tgtcagaaaa tgccggaacg agtcgcctac agccaggaac accacttcaa
     1441 ccggcgcggc catgcccgac agaatggacc cgcaggccag catttgaagc aggcgccccg
     1501 cagaccagta ctccggctgg tagacaatgc ggatgaattc cggaccggcc agcaccatgc
     1561 cccagagcac gggcagggtg acggacataa gcagcagccg gatacgcgtc gaatgcctcc
     1621 gcagcgctcc gggccgctcc cgggccattt ccgcatagac gggaaaaaga acacggttgg
     1681 acagggagcg cagcaggtct acgatggagc tggcaaacat gtacgcgatg ccgtacacgc
     1741 cgagcatgcc gacatcgaga aagcgcgcga gcaccgtgcg atccaggttg ttggccagaa
     1801 aggtgagcag ggaactcaca aagatccatt tgccgaagtg gataagctgg tgggcgtatt
     1861 cttttcgcca ggcgaagccc attcgcgggc cgggaatctg atacgtgagc agagtgtgga
     1921 aaaccatggc ggcgaggttt ccgaccacca atgcccagac gcccatccag tagaatgagt
     1981 cgtcgaactg aatctggcgc cagtcgccgc ccgcggaaaa cgtcacgaac atggcgttaa
     2041 gcgtccggac ttccgcatag atgcgccagg cgagcgcacc cgccatgctt accatcaagc
     2101 ccgccacctg cgtgataacg gaaagctgga tttgcagcgg cacgttcaca tggcggtgca
     2161 gcagatgcag gtttgtggag tcgaggctga cgatcagcag gttcaggcct gcgacgggca
     2221 ggaagtaccg cagcgcgggc tggttgaaat accaggcggc gggaagtgtg aacgccaggg
     2281 cgccaagcca gaggcccagt ccgcgcagca gttgcagcgt ccaggcggtg cgaagaaatt
     2341 cggggtcctc ggccttggga ttctgcacga tgctgggcgc gatgccgagg tcggtgagca
     2401 tcgcaagccc ctgcagatag atgtttatca tgaccatcag gccaaaggct tcacgaaaca
     2461 gcaggcgggt caaaacgata ttgccgccga gccggatggt ctgcgtgatg ccgtagccgg
     2521 caaacgtcca gacggaaccg cgaatcgtga gacgcttcag agaacgcggg cgcgcttgcg
     2581 gcgcgccggc agcctcagcg tcatgagagg gcccggaggc cgggtcatca gcaggcatgc
     2641 cgtctcccct ggttcagcgc cgtgacaaat tcaactagag tgtacggcat gccagtcttt
     2701 tctcctcccc gtgccggggc atccggtgcg cgtc
//
LOCUS       DAOPBQ010000505         2718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_29455_length_2718_cov_4.718363, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000505 DAOPBQ010000000
VERSION     DAOPBQ010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29455_length_2718_cov_4.718363"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="PKW60_13470"
     CDS             <1..1488
                     /locus_tag="PKW60_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00489.1"
                     /translation="KNSSHRASWLEHYRKSFFSVSDPYWQSFYWNQIYKLRAATRQDG
                     VILGLQGPWDQPTPWPGIWWNLNVQLTYWPMYVSNHLELAEPLPENLRRHLPQLVANV
                     PPEYRHDSAGVYRSSTARLVGGVFYPSLVERPGDEGEPEVGNLLWACHNVWLHYRMSM
                     DEQLARESLYPILRRAVNYHRHFLEEGPDGRLHLPRTRSPEFKSGPDCNYDLALFRWG
                     VETLLTITKRLGLDDPLAPEWRRIVEKLTDFPEGEQGFLIARDMPLDSSHRHYSHLLM
                     IYPLYLVNAEQPEGKMRILRSLDHWHSLPEALFGYSYTGGASIAAAIGDGQRALDCLN
                     AFRPFIEPNTMYREAGPVIETPLSAAQSIHDMLLQSWDAPDGPLLRIFPAVPELWRDA
                     VFHQWRAQGAFLVSAARSEGRLLWVEVTSLAGEPCRVQAGFSAHPRLVAGEGVSFTAL
                     EGNRYALGLSKGQTALLLDPAWDGEPEIAPVAGSLPEMPVFGLKR"
     gene            complement(1529..>2718)
                     /locus_tag="PKW60_13475"
     CDS             complement(1529..>2718)
                     /locus_tag="PKW60_13475"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00490.1"
                     /translation="VIRVGMIGCGGRNTGAAAQALTADPGARLVAMCDIFMERVKSRR
                     QRLRDQMKAQVAVDDDHCFAGFDSYKQVIEASDVVLIANAAKFHPLHAMAALQAGRHV
                     FVEKPHGIDPAGIKLMQRAADTAKEKGVSLVSGLQSRYHTGYAETVQRIHGGAIGDVV
                     CIEENFLRAPYVVIDRAPELSELEWQCSTQYHFRWLSGDDVPQSLVHNLDRASWVLHN
                     AVPARCHGLGGRSSMTAPIFGDVFDHHAIVYEFENGVKIYAFCRTTEGCYGESSSIVY
                     GTKGKASIMNAQIWGENPWRWEGACDPYQIEHDRLFKAIRTGEPLNNGGYMARSTLIG
                     VMGQLSCYTGEEITWEQASASDFYYPPAPEDCHDGMEPPVMPGADGSYPVPRPGFTRM
                     LDT"
BASE COUNT          480 a          872 c          842 g          524 t
ORIGIN      
        1 aaaaactctt cccatcgggc atcgtggctt gagcactacc ggaagagttt tttctccgta
       61 tccgatccgt actggcagag cttctattgg aaccagatct ataagctgcg tgccgcgacc
      121 cgacaggacg gtgtgatcct ggggctccag ggaccgtggg atcagccgac accctggccg
      181 gggatctggt ggaacctcaa cgttcagctc acgtactggc ccatgtacgt gtcgaaccat
      241 ctcgaactcg ccgaaccgct gccggagaac ctgcgcaggc atctgcccca actggtggcg
      301 aatgtgccgc cggagtaccg gcacgattcc gcgggggtgt accgttcgtc gacggcccgg
      361 ctcgtgggag gcgtgttcta tccgagcctg gtcgagcggc ccggcgatga gggcgaacca
      421 gaggtgggca atttgctgtg ggcctgccac aacgtctggc tgcattaccg catgagcatg
      481 gacgagcagc tcgcgcgcga gtcgctgtat ccaatattgc gccgcgccgt gaactatcac
      541 cgccatttcc tcgaggaagg tccggacgga cgcctccacc tcccgcgaac gcgttcgccc
      601 gagttcaaaa gtggtccgga ctgcaactac gacctggcct tgttccgctg gggcgtggaa
      661 acgctcctca ccatcacgaa acgcctcggg ttggacgatc cgctggcgcc ggaatggcgg
      721 cgtatcgtcg agaagctgac cgatttcccc gaaggagagc aaggatttct gatcgcacgc
      781 gacatgccgc tcgattccag ccaccggcat tactcgcacc tgctgatgat ctacccgctc
      841 tatctggtga acgccgaaca gcctgaaggg aaaatgcgga ttttgcgctc gctggaccat
      901 tggcacagcc ttcctgaggc gctgttcggc tattcctaca cgggaggcgc atccattgcg
      961 gccgccatcg gtgacggcca gcgggcgctg gactgcctca acgcattcag gccgtttatc
     1021 gagcccaata caatgtaccg cgaagccggc cccgtcatcg agacgccgct gtcggccgcg
     1081 caatccatcc acgacatgct gctccaaagc tgggacgcgc ccgacgggcc gctgctccgc
     1141 atctttcccg cggtgccgga gttgtggcgc gacgccgtct tccatcaatg gcgcgcacag
     1201 ggcgctttcc tcgtgagcgc tgcgcgttcc gaaggccggt tgctttgggt cgaggtgacc
     1261 agcctcgccg gcgaaccctg ccgcgtgcag gccggcttca gcgcccatcc acggcttgtc
     1321 gccggcgagg gcgtgtcgtt caccgcgctg gaaggcaacc gctacgcgct gggcctgagc
     1381 aaaggccaga ccgccctgtt gctcgatccg gcctgggacg gcgaaccaga aatcgcgccg
     1441 gtagcggggt ctctgccgga aatgcccgta ttcgggctga aacgctgaac agtttcgccc
     1501 ggaccgcagg gagggcggca cgcaccggtc aggtatcgag catacgcgtg aatcccggcc
     1561 ggggcaccgg gtaactgccg tcggcgccag gcatgaccgg cggctccatg ccgtcgtggc
     1621 agtcttcggg agccggggga tagtaaaaat cggacgcgct cgcctgttcc cacgtgatct
     1681 cttcgcctgt gtaacacgag agctgcccca tgacgccgat caaggtgctg cgggccatat
     1741 agccgccgtt gttgaggggt tcgcccgtcc gaatcgcctt gaacagcctg tcatgctcga
     1801 tctggtacgg atcacaggcg ccttcccagc gccacgggtt ttcgccccag atttgagcgt
     1861 tcatgatgga cgccttgcct ttagtgccgt agacaatgct ggatgattcg ccgtaacagc
     1921 cctcggtcgt gcggcagaaa gcgtagatct tcacgccgtt ctcgaattcg tacacgatcg
     1981 catggtgatc aaatacgtcg ccgaaaatcg gcgcggtcat cgaagagcgg ccgccgagtc
     2041 cgtggcatct tgccggcacc gcgttgtgga gcacccagct tgcgcggtcc aggttgtgca
     2101 cgagcgattg cggcacgtcg tctcccgaaa gccagcggaa atggtattgc gtgctgcatt
     2161 gccactcgag ctcggaaagc tccggcgccc ggtcgatgac tacgtacggc gcccgcagga
     2221 agttttcttc gatgcagact acgtcgccga tcgcgccgcc gtgaatccgc tggaccgtct
     2281 cggcataccc ggtgtggtag cggctctgca gtccggatac gaggctcact cccttttctt
     2341 tcgctgtatc tgccgcgcgt tgcatgagct tgatgccggc cgggtcgatg ccgtggggct
     2401 tttcaacaaa cacgtgccgg ccggcctgga gcgcggccat ggcatgcagc ggatggaatt
     2461 tcgccgcatt ggcgatcagc acgacgtccg aggcctcaat aacctgtttg tagctgtcga
     2521 aacccgcaaa acagtggtcg tcgtcgacgg ccacctgcgc tttcatctga tcccggagcc
     2581 gctggcgccg gctttttacg cgctccatga aaatgtcgca catagccacg agccgtgcgc
     2641 ccgggtccgc cgtcaaggcc tgggcggctg cgccggtgtt gcgcccgcca cagccgatca
     2701 tgcctacgcg aataacat
//
LOCUS       DAOPBQ010000506         2718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_29458_length_2718_cov_3.294029, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000506 DAOPBQ010000000
VERSION     DAOPBQ010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29458_length_2718_cov_3.294029"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2718
                     /locus_tag="PKW60_13480"
     CDS             <1..>2718
                     /locus_tag="PKW60_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00491.1"
                     /translation="DQRNSGSLIIRDAIAERDRQGLLPLQDWMPLFSFEMISAAPPDA
                     TKSLGGGAMEKAFLRDPLNDRVFSSQSPLTFTISPDEDTNNGPALIQTDILEFGIFRE
                     KPTWDGGTHGVLESDEPLVLRFLPDGTVVNTVTGVPYDMREGPTYPDPSPGAGVQYVG
                     RDRLGLRYKLWGQRSRPPAGGGLIDVYPAIQSRYYSVDDSGASYILAVRTSATWSSLQ
                     ELSYYVDSYMMVDGFGLMPVDENGELIDTYDPDFDALPFDPDTTYLSQFRVDDATSGL
                     FDDFRGESDGNAWYWPQRMYTPIQEYVRPRWDVPGGSFANAFDFVTGEWLNLRKLHSL
                     EGWIQMVAIDVHASNPPNTLGARAKHGEWSDSGDWVAFKEINLIISDVRGDPSDPKGV
                     NRLNPIEEFNSLTYDALIEGGNIITNLAVGNDFAFNGVWVFHDSNNNGVFDPPIPQPD
                     GRGVTFVDYPMLPNNYIIVEDAQSSGGGTKSGEKSSSPLATGPLSGEKARDVNYYPPE
                     WEYIPFPPGGGAPWWKVRLVMGGGSREGVDKGFVEPVPDFPDPDTHDTRYDYFVVVRP
                     DSGYFDLSTMPGDGVGMTLGMDYRVFIEPRRFNPLVLDNGPYGSPNGSWDGGLYFDSM
                     QMPMGRRDDSPTVDFAQNIWQNDPLWLEYEPWWPERTADRTTAKPARVGLEVHDLTLT
                     YESNSKYATQTNMIYANGWPLWLDPFGQNDARFYKGAHVFVQADAIVTEGPADEDLAP
                     FQHAFESAPFFQPNFDAAPIGPRSRVFPNPPAQPERPEYGTWPALLQPDEYPKLADWP
                     AANRRARILKQHIDSSSKPTAMLGINVAGANDPVVNRFNRLQLQQITVAFWGPDFTPS
                     DLQALDIDGVNPESGVLLYEDADSDSVFNSEFGSDAPVPLRNLMWRPEPE"
BASE COUNT          541 a          791 c          868 g          518 t
ORIGIN      
        1 gaccagcgga acagcgggtc cctgatcatc cgcgacgcca tagcggagcg ggatcggcag
       61 gggctcttgc cgctgcagga ctggatgccg ttgttcagtt ttgaaatgat cagtgctgcg
      121 ccgccggacg ccaccaagag cctgggcgga ggcgccatgg aaaaggcctt tctccgggat
      181 ccgctcaatg accgcgtgtt cagctcgcag agcccgttga cgttcaccat ctccccggat
      241 gaggacacca acaacggccc ggcgctgatt cagaccgaca ttctggaatt cggcatcttc
      301 cgggaaaagc cgacgtggga tggcggcacg catggcgtgc ttgaaagtga tgagccgctg
      361 gtgctgcggt ttcttccgga cggcacggtg gtgaatacgg ttacgggcgt gccgtatgac
      421 atgcgcgaag gcccgacgta tcccgacccc agcccgggcg ccggtgtcca gtatgtcggc
      481 cgggaccggc tgggcctgcg ctacaaactc tggggtcaga ggagccgtcc ccccgcgggc
      541 ggcgggctga ttgatgtgta tcccgcgatc cagagccggt attactccgt cgacgactcc
      601 ggcgcatcgt acattcttgc ggtgcggaca agcgccacct ggtccagcct gcaggaactc
      661 agctattacg ttgactccta catgatggtg gacgggttcg ggctgatgcc ggttgacgaa
      721 aacggagagt tgatcgatac ctacgacccg gatttcgacg cgcttccgtt tgatcccgat
      781 acaacctacc tttctcagtt ccgggtggac gacgcgacaa gcggcctgtt tgacgatttc
      841 cgcggggaat ctgacgggaa cgcgtggtac tggcctcagc ggatgtacac gccgatccag
      901 gaatacgtgc ggccgcgatg ggatgtgccg ggaggctcgt tcgccaatgc ctttgatttt
      961 gtgacgggcg aatggctcaa cctgcgcaaa ctgcattcac tggagggatg gattcagatg
     1021 gtggcgatcg acgtccacgc atcgaatcca ccgaatacgc tgggggcacg cgccaaacat
     1081 ggtgagtggt cggatagcgg cgattgggta gcgtttaagg aaatcaacct gatcatcagc
     1141 gatgtccgcg gcgatccctc tgaccccaaa ggcgtgaaca ggctgaaccc catcgaggaa
     1201 ttcaattcgc tgacctacga cgcgctgatt gaaggcggga atatcattac caaccttgcg
     1261 gttggcaacg atttcgcgtt caatggcgtc tgggtcttcc atgacagcaa caacaacggc
     1321 gtctttgatc cgccgattcc gcagccggac ggccggggtg tcacttttgt cgactacccg
     1381 atgctgccga acaattacat cattgtagag gacgcccagt cgagcggcgg gggaacaaag
     1441 tcgggcgaga agtcgtccag tcccctcgcc acgggacccc tgagcggcga gaaagcccgc
     1501 gatgtcaatt actacccgcc cgaatgggag tatatcccgt tcccgccggg cggcggcgcc
     1561 ccgtggtgga aagtccgtct ggtcatgggc ggcggctcgc gcgaaggggt agacaaagga
     1621 tttgtcgagc cggttcccga ttttccggat ccggacacgc acgatacccg atatgattat
     1681 tttgtggtgg tgcggcctga cagcggatac ttcgacctgt cgactatgcc cggcgatggc
     1741 gtgggcatga cgctgggcat ggattaccgt gtgttcatcg agccgcgccg gtttaatccg
     1801 ctggtgctgg acaacggccc gtacggcagc ccgaacggca gttgggacgg cggtctgtac
     1861 ttcgactcga tgcagatgcc gatgggtcgt cgcgatgact cgcccaccgt ggactttgcg
     1921 cagaatatct ggcagaacga tccgctgtgg ctggaatacg agccgtggtg gcccgagcgg
     1981 accgcggacc gaacgactgc gaagccggcg cgtgtcgggc tggaagtcca tgacctgacc
     2041 cttacgtacg agagcaacag caagtacgcc acgcagacaa atatgattta tgcgaacgga
     2101 tggccgctct ggcttgaccc gttcggacag aacgacgcgc ggttctacaa aggcgcgcac
     2161 gtttttgtgc aagcggatgc gatcgtaacg gaaggcccgg ccgacgaaga cctggcgccg
     2221 ttccagcacg cattcgagag cgccccgttc ttccagccga actttgatgc ggcgccgatc
     2281 ggtccgcgct cacgcgtgtt cccgaacccg ccggcgcagc ccgagcggcc ggaatatggg
     2341 acctggccag ccttgctgca gcccgatgaa tacccgaaac tggcggactg gcctgcggcc
     2401 aaccggcgag cacgtatttt gaaacagcat atcgactcga gcagcaagcc tacggcgatg
     2461 ctcgggatca atgtggccgg cgcgaacgac ccggtggtca accggtttaa caggctgcaa
     2521 ctgcagcaga tcacggtcgc attctggggg ccggatttta cgccaagcga tctgcaggcc
     2581 cttgatattg acggcgtcaa tcccgaatcc ggtgtgctgc tgtatgaaga tgccgacagc
     2641 gattcggtat tcaacagcga attcggcagc gatgcgccgg taccgttgcg gaacctcatg
     2701 tggcggccgg aaccggag
//
LOCUS       DAOPBQ010000507         2714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_29517_length_2714_cov_3.612636, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000507 DAOPBQ010000000
VERSION     DAOPBQ010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29517_length_2714_cov_3.612636"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PKW60_13485"
     CDS             complement(<1..717)
                     /locus_tag="PKW60_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPK00492.1"
                     /translation="MFEHTFMQHAALAGLCAGISCGLMGVFVVIMHLSMLGICIAHAA
                     FAGAMLGILLGFEPLWGALVFSLAAAAFVGPLADRGQLNPDSAVGIVFSFMLGLAFLF
                     VGLTPGAKTHALSLFWGSILTVSTTDLAYLAAVTALIVVLLVLFFKEIQAVVCHRRVA
                     FAVGIPATVVFYGLLFATGATVAVSLRTVGGLLIYSLLLNPAAAAFQLTFSLKWMFFL
                     AAAFGVFSCWLGLAGSYFWDL"
     gene            complement(726..1481)
                     /locus_tag="PKW60_13490"
     CDS             complement(726..1481)
                     /locus_tag="PKW60_13490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPK00493.1"
                     /translation="MQPDAIVLENVSVTAHHATILHNLSLRVAHGSFVGVLGPNGAGK
                     TTLLNVINGLIRPSSGEVTVLGKKPFQWEGHRLRSEIGRVAQAEPVDRRLPITVRETV
                     LVARYGRLGWLRRPGPGDHARVNEALAAVGMESFATRPLGHLSGGEYQRVAIARALAQ
                     DPRLFLFDEPTASIDPRVQSEIVALVEEIHAARGVTSLYVTHDLATLPKTCETVILLR
                     EGRIWASGPREAMLEEARLAALYARNSATGTPA"
     gene            complement(1498..2325)
                     /locus_tag="PKW60_13495"
     CDS             complement(1498..2325)
                     /locus_tag="PKW60_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPK00494.1"
                     /translation="MLTGCVAVLCALSGCAEHTAPVVAGTALLQCAVQDAGAAGAAPS
                     VLVPPGSCPGHYDMRPSDVARVAHCDALFLHPWQELMPSVTRLIEASGIASEKVVVIP
                     VPGNWMLPDVYAQALEAVSSALTTRGLASGRARDHIERRCAEVRALGDALRDQLIAAG
                     APETPVICQEMIAPVVEWAGFPVAGIFARSEDLSVSGVQALVQRSKGEAVALVIDNLQ
                     SGDEKMGAALAAELGTQCVVISNFPGGLPETDTWDQAVAENVERMLNALRQIREPAA"
     gene            complement(2363..>2714)
                     /locus_tag="PKW60_13500"
     CDS             complement(2363..>2714)
                     /locus_tag="PKW60_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00495.1"
                     /translation="AKLAWTAIIGKGYGGGSKVLRLREGIERFSVTDINNPAASALAQ
                     SELVVMFDTYGSFADSASAAGGVIFNHIPGGCNILYMDGHVNFIRYPQQFPIVDDAAN
                     GYGIPRQIGHYGLG"
BASE COUNT          567 a          875 c          840 g          432 t
ORIGIN      
        1 aaggtcccag aaataggacc ctgccaggcc cagccagcac gaaaatacgc caaaagcagc
       61 tgccagaaaa aacatccatt taaggctgaa ggtcagctga aatgcggcgg ctgcagggtt
      121 cagaaggagg ctgtaaatga gcagtccccc gaccgtcctc agggagacgg ccacggtggc
      181 gccggtcgca aacagcagac cataaaatac tacagtggcc ggaatcccca cggcaaatgc
      241 cacgcggcgg tggcagacca ccgcctggat ctccttgaaa aacagcacga gcagcaccac
      301 gatgagggcg gttacggccg cgagatacgc cagatcggtc gtggatacgg tcaatatgct
      361 cccccagaac aggctgaggg cgtgcgtctt cgcaccgggc gtcaggccga cgaagaggaa
      421 ggccagcccg agcatgaacg agaacacaat gcccacggcg ctgtccgggt tgagctgtcc
      481 ccggtcggcc aacggcccga caaacgccgc cgccgccagg ctgaacacca gggcgcccca
      541 gaggggttcg aagccgagga ggatgccgag catggccccg gcgaatgccg catgggcaat
      601 gcagatgccc agcatcgaca ggtgcatgat cacaacaaaa acgcccatca atccgcagct
      661 aatgcccgcg cacagcccgg ccagtgcggc gtgttgcatg aatgtgtgtt cgaacatggc
      721 tcccatcagg cgggcgtacc cgtggctgag ttcctggcgt acaacgccgc cagccgcgct
      781 tcttcgagca tggcttcgcg cggaccactg gcccagattc gcccttcgcg cagcaggatc
      841 accgtctcgc aggtcttggg cagcgtggcc agatcgtgcg tcacgtaaag cgacgtcact
      901 ccgcgggcgg cgtggatctc ctcgaccagt gccacgattt cggactgtac ccgcggatca
      961 atggatgcgg tcggttcgtc gaagaggaag aggcgcggat cctgtgcaag cgcgcgtgcg
     1021 atggccacgc gctgatattc tccgccggat aaatgaccca gaggccgggt ggcgaacgat
     1081 tccatgccga ccgccgcgag cgcttcgttc acgcgcgcgt ggtcgccggg acccggccgg
     1141 cgcagccatc cgagccgtcc gtagcgggct accagtaccg tttcgcgcac ggtaatgggc
     1201 aggcgccgat ccaccggctc agcctgggcg acgcgtccga tctcggaccg gaggcggtgc
     1261 ccttcccatt ggaacggttt tttgccgagc acggtgacct cgcccgaaga cgggcggatc
     1321 aaaccgttga tgacgttcaa cagcgtcgtc ttgcccgcgc cattcgggcc cagaacgccc
     1381 acaaaggagc cgtgcgcaac ccgaagcgac aggttgtgca gaatggtcgc atggtgcgcc
     1441 gtaaccgata cattttccag aactattgca tccggttgca tgaggccgcc cccccggcta
     1501 cgccgcgggt tcgcggatct gccgcagggc gttcagcatg cgctcaacat tctccgcaac
     1561 agcctggtcc caggtgtcgg tttccggcaa tcctccggga aaattcgaga tgaccacgca
     1621 ctgagttccc aattcggcgg ccagcgccgc gcccattttc tcgtcgccac tttgcaggtt
     1681 atcaatgacg agagcgaccg cctcgccctt gctgcgctgc accagcgcct ggacgccgct
     1741 cacactgagg tcttccgaac gggcgaagat tccggcaacc ggaaatccgg cccattcgac
     1801 gaccggagca atcatttcct ggcagatcac cggcgtttcg ggcgcgcccg ccgcaatcag
     1861 ctgatcccgg agcgcatcgc ccagcgcgcg cacttcggcg cagcgtcgtt caatgtgatc
     1921 ccgtgcccgg cctgaggcga gtccgcgggt ggtaagtgcg gacgagacgg cctccagagc
     1981 ttgagcatac acgtccggaa gcatccagtt gccgggcacg ggaatcacaa cgaccttttc
     2041 ggaagcaatg cccgaagcct caataagccg cgtaacgctg ggcataagct cctgccaggg
     2101 gtgcagaaaa agggcgtcgc aatgggcaac ccgggcgacg tcgctcggcc gcatgtcgta
     2161 atgaccggga caacttcccg gagggaccag cacggacggc gccgcgcctg cagcgcccgc
     2221 gtcctgaact gcgcattgca gcagtgccgt gccggccacc accggagcag tgtgttcagc
     2281 gcaaccggaa agcgcgcaga gaaccgccac gcagcctgta agcatgccaa accacgcgca
     2341 tccgcggcgt ttccgctggc aatcatccaa gaccatagtg gcctatctgg cgagggatgc
     2401 catagccgtt cgcggcgtcg tcaacaatcg ggaactgctg gggatagcga atgaaattga
     2461 cgtggccgtc catgtacagg atattgcacc cgccggggat gtggttgaag atgaccccgc
     2521 ccgcagcact ggcggaatcc gcgaagctgc cgtaggtgtc gaacataacg accagttcgc
     2581 tctgggccag agccgaagca gcgggattat tgatgtcggt gactgagaaa cgctcaatgc
     2641 cctcgcgcaa gcgaagaacc ttggagccgc cgccatatcc tttgcctatg atcgcggtcc
     2701 atgcgagctt cgct
//
LOCUS       DAOPBQ010000508         2713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_29536_length_2713_cov_3.640331, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000508 DAOPBQ010000000
VERSION     DAOPBQ010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29536_length_2713_cov_3.640331"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /gene="araD"
                     /locus_tag="PKW60_13505"
     CDS             complement(<1..108)
                     /gene="araD"
                     /locus_tag="PKW60_13505"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667827.1"
                     /note="catalyzes the isomerization of L-ribulose
                     5-phosphate to D-xylulose 5-phosphate in the anaerobic
                     catabolism of L-ascorbate; links the arabinose metabolic
                     pathway to the pentose phosphate pathway and allows the
                     bacteria to use arabinose as an energy source; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HPK00496.1"
                     /translation="MLDDLKERVWRANRDLVARGLVFATWGNASAFDRAS"
     gene            complement(186..1190)
                     /gene="dusB"
                     /locus_tag="PKW60_13510"
     CDS             complement(186..1190)
                     /gene="dusB"
                     /locus_tag="PKW60_13510"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPK00497.1"
                     /translation="MRIGTITLDKPLALAPMEDVTDYSFRTICKEMGADLLYTEFASS
                     EALIREAGKTLDKIRIRDEERPIAVQIFGNAESSMEGAAQVAEQAGPDFVDVNCGCWV
                     KKVALRGAGAGLLQDIRKFERVVRGVLAGTSLPVTVKTRLGWDADSIIILDVARMLED
                     LGVQALTVHCRTRKQGHSGQADWGWLERLKNTVSLPIIGNGDVTSPEDVKRMFETGCD
                     GVMIGRGAIQNPWIFRQAKHYLATGEALEPPSVPERVALCISHLRRSIGVKGERKGIV
                     EFRKHYSGYLKNLPHVSRLRLELMEMYDLESIEERLCRFRDEYAELQPDAALLPDAPG
                     "
     gene            complement(1232..>2713)
                     /gene="thiC"
                     /locus_tag="PKW60_13515"
     CDS             complement(1232..>2713)
                     /gene="thiC"
                     /locus_tag="PKW60_13515"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPK00498.1"
                     /translation="PERPRGVYRAKPGARATQMDCAKRGEITPEMEYVAIRENARWQD
                     APEELKRRHPGEAWGARIPDEITPEFVRDEVARGRAIIPANVNHPELEPMIIGRNFKV
                     KINANLGNSAHSSSVHEEVEKMLWAIRWGADTVMDLSTAEPIQATRESILRNCPVPVG
                     TVPIYEALERVGGAPEDLTWEVFRDTVIAQAEQGVDYMTIHAGVLLPFIHLAARRTTG
                     IVSRGGSIMGRWCLAHHKENFLFTHWDELCEIMASCDVAFSIGDGLRPGSLADANDEA
                     QFAELKAQGGLTRRAWEFGVQVMNEGPGHVPLHLIKENMDRQLEWCGEAPFYTLGPII
                     TDISPGYDHLASAIGAALIGWYGTAMLCYVTPKEHLGLPDRNDVREGVVAYRIAAHAA
                     DLAKGHPAAQIRDNALSKARFEFRWEDQFNLSLDPDRARAFHDETLPQRGEKLAHFCS
                     MCGPKFCAMALSRQVRDEAFIQQGLQEQAAAFKEGGSALYRKV"
BASE COUNT          497 a          880 c          781 g          555 t
ORIGIN      
        1 gctcgcacgg tcaaaggcac tggcgttgcc ccaggtcgca aatacgaggc cacgggcgac
       61 caggtcgcgg ttggcccgcc acacccgctc tttcaggtcg tcaagcatcc aacgtatccc
      121 ccagcctttc cgcatccgcc gtacgagggc atcgcggttt cacgcacagc gcagaacgag
      181 aatactcagc caggcgcatc gggaagcaag gccgcatctg gctgaagctc cgcgtactca
      241 tcacggaagc gacacagtcg ttcctcaatg gattcgagat cgtacatttc catcaattcg
      301 aggcggagtc tggacacgtg cggcaggttc ttgaggtatc cgctgtagtg ttttcgaaac
      361 tcgacgatcc ctttccgctc ccctttcact ccaatgctgc gccgcagatg gctgatacac
      421 agcgcgacgc gttcagggac cgacggtggt tcaagcgctt cgccagtagc cagataatgc
      481 ttcgcctgcc tgaatatcca ggggttctgt atggcccccc ggccgatcat gacgccgtca
      541 cagcccgtct caaacatgcg cttgacgtct tccgggctgg tgacatcccc attgccgatg
      601 atcggcaggc tgaccgtatt cttcagacgt tcgagccagc cccagtcggc ctgtccgctg
      661 tgcccctgct tccgggtcct gcaatggacc gtcaacgcct gcacgcccag atcttcgagc
      721 atacgggcaa catccagaat gatgatgctg tcggcgtccc agcccaggcg tgtttttacg
      781 gtgacaggca gggaggtgcc ggcaaggacg ccccgcacaa cgcgttcaaa tttccggatg
      841 tcctgaagca atccggcgcc ggctccgcgc agagcgactt tttttaccca gcagccgcaa
      901 ttgacgtcga caaaatcggg gcctgcctgt tcggcgacct gcgctgcacc ttccatcgag
      961 gattccgcgt tgccgaagat ctgcacagca atggggcgct cttcgtcgcg gatgcggatc
     1021 ttgtcgagcg tttttccagc ttcgcgtatc agggcctccg agcttgcaaa ctcggtgtac
     1081 aggaggtccg cccccatttc tttgcagatc gtccggaatg aataatccgt gacatcctcc
     1141 atgggcgcca gagcaagagg tttatcgagt gtgatcgtgc caatacgcat ggtgtcgctg
     1201 cttttgccgg acaggcctcc tccgaacggg attatacctt acggtagagc gccgagccgc
     1261 cttctttgaa cgcggccgcc tgctcctgca gaccctgttg gatgaacgcc tcgtcccgga
     1321 cctgccgcga gagagccatc gcgcagaact ttggtccgca catggagcag aaatgcgcca
     1381 gtttttcccc gcgctgtggc aaagtctcat cgtgaaaggc acgggcacgg tcggggtcga
     1441 gcgacaggtt gaactgatct tcccaccgga actcgaagcg agccttgctc agggcattgt
     1501 cacgaatctg cgcggccgga tgaccttttg ccaggtctgc cgcatgtgcc gcaatccggt
     1561 acgcgaccac tccctcccgc acgtcattgc ggtcgggcag tcccaggtgt tctttcggcg
     1621 tcacgtaaca caacatggcg gtgccgtacc acccgatcag cgccgcgccg atggccgatg
     1681 ccaggtggtc gtagccgggg gaaatgtcgg taatgatcgg gcccagggta taaaacggcg
     1741 cttcgccgca ccattccagc tgccggtcca tgttctcttt gatgaggtgc agggggacgt
     1801 ggcccggacc ctcattcatg acctggacgc cgaattccca ggcgcgccgg gtcaaaccgc
     1861 cctgtgcctt caattccgcg aactgcgctt cgtcgttggc gtcggccagg ctgccggggc
     1921 gcaggccatc tccaatggaa aacgcgacat cgcaagacgc catgatctcg cagagttcgt
     1981 cccagtgggt aaacaggaag ttttccttgt ggtgggcgag gcaccaccgg cccatgatcg
     2041 agccgccccg gctcacaatg cctgtggtcc ttctggcagc cagatggata aacggcagca
     2101 gcacacccgc gtgaatggtc atgtagtcga cgccctgctc cgcctgcgcg atgaccgtat
     2161 cgcgaaacac ctcccacgtc aggtcttccg gagcgccgcc tacccgctcg agcgcctcgt
     2221 aaatgggaac cgtgcccacc ggtacgggac aattacgaag gatcgattcc cgggtagcct
     2281 ggatgggctc cgcagtcgac agatccatca ccgtgtcggc gccccagcgg atcgcccaca
     2341 acatcttctc gacttcttcg tgcaccgagg acgaatgcgc ggaattgccc aggttggcgt
     2401 tgatttttac cttgaagttg cggccaataa tcatgggctc cagttcaggg tgattcacat
     2461 tggcgggaat gatcgcccgg cccctggcga cctcatcgcg cacgaattca ggcgtgatct
     2521 cgtccggaat ccgcgcgccc catgcctctc ccgggtggcg gcgcttcagt tcttcagggg
     2581 cgtcctgcca ccgggcgttc tcgcgtattg ccacatattc catctccggc gtgatctcgc
     2641 ctcgtttggc gcaatccatc tgcgtcgcgc gcgcccccgg ttttgcacga tacacgccgc
     2701 gcggccgctc tgg
//
LOCUS       DAOPBQ010000509         2704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_29643_length_2704_cov_3.566251, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000509 DAOPBQ010000000
VERSION     DAOPBQ010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29643_length_2704_cov_3.566251"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..2510)
                     /locus_tag="PKW60_13520"
     CDS             complement(84..2510)
                     /locus_tag="PKW60_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPK00499.1"
                     /translation="MVIGLERIIVFLVLFAASTAALAASDYPIQPVPFTRVKIDDPFW
                     SPRQTTCLDVTIPYCFQKCEETHRIDNFAVAAGMKDGTFEGIFFNDSDVVKVVEGAAY
                     ALALRSDPKLDAYLDDLIAKIAGAQEPDGYLYTCRTIDPENPPQGAGQTRWSNLKVSH
                     ELYNIGHMYEAAVAHHQATGKASLLEVARKSADLVDRVFGPDGIRNVPGHEEIEIGLC
                     RLYRETGDERYLQLARFFVDERGQENGRTRFGEYFQDHLPILEQTEAVGHSVRAGYFY
                     SGTADVAALTGESAYLAPLDRIWNDAVSRKMYITGGTAAQRQGEAFGPAYFLPNKEAY
                     NETCAAIANIFWNHRMFLLHGHAQYIDVLERTLYNGFLVGVSLDGDTFFYPNPLESDG
                     VYGFNHGSTSRSPWFSTSCCPVNVARFVPSLANYIYGQRGREVFLNLFIADSAELTVA
                     GVPVTLTQQGRYPWDGAIEIAVEPDSPVEFTLNVRIPGWAREEPLPSDLYRYQTPSSA
                     RCELSVNSEPQRLELVNGYVPVTRSWKKGDTLSLRLPMPIRRVLAHEKVEADRGRVAI
                     ERGPIVYCAEGVDNGGQARNLVLPDDAVLAAEHRASLLDGVTILKGKAFKRQRNEAGE
                     ETVDETELTLVPYYGWAHRGVGEMQVWLARTPDIAELPPIPTVASSAVPSASHVNPSD
                     SVAALNDQQEPQNSNDHSIPRFTWWDHRGTNEWVQYEFQEPRAVASVDVYWFDDSGVG
                     QCRVPAAWHLEYRDGGAWKSVENPSGYEAAKDAWAKTAFTPVTTDALRLVVQLQPDFS
                     GGILEWRVNE"
BASE COUNT          535 a          795 c          826 g          548 t
ORIGIN      
        1 ccagaacgac gcttgcgacc cgggaccaat cacacgccat cacaacatcc tcccttgccc
       61 atgcgggatt gcacaacagc cggctattcg ttcactcgcc attcgaggat tccaccggag
      121 aaatccggct gcaactgcac aaccagccgg agcgcatctg tggtgactgg tgtaaacgcc
      181 gtttttgccc atgcatcttt ggcggcttcg tagcctgagg gattttcaac tgatttccag
      241 gcgccgccgt cgcggtattc cagatgccag gccgcaggca cgcggcactg gcccacaccg
      301 ctgtcgtcga accaatagac gtccaccgac gccacagccc gaggttcctg aaactcgtat
      361 tgaacccatt cattcgttcc ccggtgatcc caccatgtga agcggggaat gctgtgatcg
      421 ttggagtttt gcggctcctg ctgatcgttt agggcggcca cgctgtcact ggggttcacg
      481 tgagaggccg aggggacggc ggatgatgcc acagtgggaa tgggggggag ttcggcaata
      541 tccggcgttc tcgccagcca gacctgcatt tcgcccacgc cccggtgcgc ccagccataa
      601 tagggcacca gagtgagctc agtttcgtcg acggtttcct ctccagcttc attgcgctgg
      661 cgtttgaacg ccttgccttt gaggatggtg acgccatcga gcagggaggc ccggtgctcc
      721 gcggcgagaa cggcgtcgtc gggcaagacc aggtttcgtg cctggccgcc gttatcgacg
      781 ccttccgcgc agtacacgat cggtccgcgt tcaatcgcta cgcgtccgcg gtccgcctca
      841 accttttcat gtgccagaac ccgacggatc ggcatcggaa ggcgcaggct gagggtgtcg
      901 cctttcttcc acgagcgcgt cacgggtacg tacccgttga caagttccag ccgctgtggc
      961 tcgctgttga cgctgagttc gcagcgtgcg gagctgggcg tctgatagcg gtacagatcg
     1021 ctggggagcg gttcttcacg tgcccagccg ggaatgcgca cgttcagcgt gaattccacg
     1081 ggcgagtccg gttcaacagc gatttcaatg gcgccgtccc acgggtaacg accctgctgc
     1141 gttaaagtca cgggcacccc ggcaaccgtc agttcggcgg agtctgcgat gaagagattc
     1201 aaaaatacct cccggccgcg ctgcccgtag atgtaattag ccagcgacgg cacaaagcgg
     1261 gcgacattga ccgggcaaca cgaggtactg aaccaggggg agcgcgacgt ggaaccgtga
     1321 ttgaatccgt aaacgccgtc gctttccagc ggattggggt agaaaaatgt gtcaccgtcg
     1381 agcgagacgc cgacgagaaa gccgttgtac agcgtgcgtt caagcacgtc gatgtattgc
     1441 gcgtgcccgt gcagcagaaa catgcggtgg ttccagaaaa tattggcgat ggcggcgcag
     1501 gtctcattat aggcttcctt attggggagg aaataggccg gtccaaacgc ctcgccctgc
     1561 cgctgggcgg cggtcccgcc tgtgatatac atttttcgcg agaccgcgtc gttccagatg
     1621 cggtcaagcg gggccaggta ggccgattca cctgtcagcg ctgcgacatc ggccgtgccg
     1681 ctgtagaaat agccagccct tacagaatgg cccacggcct cggtctgctc gagtatgggc
     1741 aggtgatcct gaaagtactc gccaaaccgg gtccggccgt tttcctggcc tcgttcatcg
     1801 acgaaaaagc gggccaattg aaggtagcgt tcatctccgg tttcccggta gaggcggcac
     1861 aggccgatct cgatttcctc atgcccgggc acattgcgaa tgccgtccgg cccgaacacg
     1921 cgatcgacca ggtctgcgga ttttcgggcc acctcgagca gcgaagcttt gccggtggcc
     1981 tgatggtgtg ccaccgcggc ctcgtacatg tgaccgatat tgtagagttc gtggcttacc
     2041 ttgaggtttg accagcgggt ttgaccggcg ccctggggcg gattctcagg atcgatggtg
     2101 cggcaagtgt agagatagcc gtccggttcc tgggcgcccg cgattttcgc gatcagatcg
     2161 tcgagatagg cgtccagctt cgggtcactg cggagtgcga gggcataggc cgcgccttcc
     2221 acaactttca ccacatcgga gtcattgaag aaaattccct cgaacgtccc gtctttcatc
     2281 cccgcagcca cggcgaaatt gtctatccgg tgcgtttcct cgcatttttg aaaacagtag
     2341 gggatggtca cgtccaggca cgtcgtctgc cgtggcgacc agaacgggtc gtctattttc
     2401 acccgcgtga acggcacagg ttgaatggga tagtcgcttg cggccagggc cgctgtcgac
     2461 gctgcgaaca ggaccaggaa tacgattatc cgctccagac cgataaccat agtaatccgc
     2521 ctccattcga tggaaatgtc gtggaaaccg cattgagaga tccgaaaacc gcgcatggtg
     2581 cggttcagat cgggaacgat aaaagcctat cgcaaaggcg ggagggactc aagcgcgtga
     2641 cggatcgtca gcggaccgca acgtaggggg agacatcggc caggcgctta tcgccaatgc
     2701 catt
//
LOCUS       DAOPBQ010000510         2695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_29786_length_2695_cov_4.975758, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000510 DAOPBQ010000000
VERSION     DAOPBQ010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29786_length_2695_cov_4.975758"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..2326)
                     /gene="fusA"
                     /locus_tag="PKW60_13525"
     CDS             complement(290..2326)
                     /gene="fusA"
                     /locus_tag="PKW60_13525"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPK00500.1"
                     /translation="MAIVDPANVRNVGILGHSSTGKTMLIEHILHLAGKTNRLGKIED
                     GNTVGDYLQEEIDRQQTICMKLMNLEWAGKRIHLVDHPGYADFVGEIAASVPVLDSVI
                     IVVDATSPIQVGTDVAMKYADKHDTPRAIFVNKLDREHTSFDEVVAAIQATYGQQCVP
                     FVLPIGHSDSLSGAVNILTEQNDELAGRIEELKASMIDVVAESDDALLEKFLESGELT
                     PDEFNLGLQKGIQAGKIVPIIGGSVSKEVGLKHLMDLVATIFPSPCDRKITATARGDE
                     EKVIEPSKDAPFLAQVFRSIVDPYVGQLTFFRVFAGTLRSDTDFYNVSTRSKERTGKL
                     YLMNGKEQTPVDSVGPGDLAAMTKLKNTHFSDTIAAAGTELTLPAIALPESMVKLAIS
                     PKSRADEDKIGEALNRMAEEDPTFTHYRDTATNEHVIRGMGDLQLDILLERMKRKFNV
                     EAETRTPKVAYKETVRGKSEVQGKHKKQTGGHGQYGDVHLRIAPNDRGAGYEFIDSIV
                     GGVVPRQYIPHVDKGCQDALEKGVIAGYPVVDIKVELFYGSYHDVDSSEMAFKIAASM
                     AIQKGVREANPCLLEPIVVLEVVVPDEFMGDVNGDLNSRRGRIMGMEPAGPGRQMVRA
                     QVPEAEILRYSTDLRSMTGGRGSYRQRFDHYEEVPEHVAQQIIAAYEKARAQGE"
BASE COUNT          494 a          843 c          759 g          599 t
ORIGIN      
        1 tcaaccagcg caattccctt atggatcttt gtgttcttcg cagacataga ggcaaatagt
       61 atatcaaccc gcgcagtcaa aagcgaaatc gtcgatgccg cctctggaac acgcgcctgg
      121 tattttcgcc gggcgccgcc gggcgcccgc gcggcgtctt ttttcacgga gctgcggttg
      181 aaatcccggt accccgttca cctggcccag gagttgcttc ccgacggaca gaaaagggga
      241 gaggcccgac gggaacctct ccccagagca cacggcctct gaaaccagct cattcgcctt
      301 gcgcccgggc cttttcgtag gcggcgataa tttgttgcgc cacgtgctcg ggcacttctt
      361 cataatggtc gaagcgctgc cggtagctgc cccggccgcc cgtcatgctg cggaggtcag
      421 tcgaataccg caggatctcc gcctccggca cctgggcgcg aaccatctgg cggccgggcc
      481 cggccggctc catgcccatg attcgcccgc gacggctgtt cagatcacca ttcacatcgc
      541 ccatgaactc gtccggaacg accacctcca gcacgacgat cggctccagg agacacggat
      601 tggcctcccg gacccctttc tggatggcca tcgatgcagc aattttaaat gccatttccg
      661 acgagtcgac gtcgtggtag gagccgtaga agagttccac tttgatgtcc actaccggat
      721 aaccggcgat cacgcctttt tcgagtgcat cctggcagcc tttgtccacg tgtggaatat
      781 actgccgtgg gacgacgccg cccacgatac tgtcgatgaa ctcatagccc gcgccccggt
      841 cgttcggagc gatccgcagg tgcacgtctc cgtactggcc gtggccaccg gtctgtttct
      901 tgtgtttgcc ctggacttct gatttgccgc gcaccgtctc tttataggcg actttgggcg
      961 tgcgcgtctc ggcctccaca ttgaacttcc gcttcatgcg ctccaggagg atgtccagct
     1021 gcaagtcgcc cattccacgg atcacgtgtt cgtttgtggc ggtgtcccgg taatgcgtga
     1081 acgtcggatc ttcttcggcc atccggttca gggcttcgcc aatcttgtcc tcgtccgcgc
     1141 gcgacttggg cgagatggcc agtttaacca tggattccgg cagggcgatc gcgggaagcg
     1201 tcaattcggt gcccgccgcg gcaatggtgt cgctgaaatg ggtgtttttc aatttggtca
     1261 tggccgccag gtcgcccggg cctacgctgt ctaccggtgt ctgctctttg ccattcatca
     1321 ggtacagttt accggtgcgt tcctttgatc gcgtgctcac attgtaaaaa tccgtgtccg
     1381 agcgcagcgt ccccgcgaac acccggaaaa aagtgagctg cccgacgtag gggtccacga
     1441 tcgagcggaa cacttgcgcc agaaaaggcg cgtccttgga aggctcgatg accttctctt
     1501 cgtcgccacg agccgtcgcc gtgattttcc ggtcgcaggg tgacgggaaa attgtggcca
     1561 ccaggtccat cagatgtttc aggccgacct ctttcgaaac gctcccgccg ataataggaa
     1621 cgatcttgcc cgcctggatg cctttctgaa gccccaggtt gaattcgtcg ggcgtcagtt
     1681 ctcccgattc gagaaacttt tcgagcaacg catcgtccga ttcagccacg acgtcgatca
     1741 tcgaggcctt caattcttcg atccggccgg cgagctcgtc gttttgctcc gtcaggatat
     1801 tgaccgcgcc actcaggctg tcagaatgcc caatcgggag gacaaagggc acgcactgct
     1861 ggccgtacgt ggcctggatg gccgctacca cctcgtcgaa gctcgtatgc tcgcggtcga
     1921 gcttattcac gaagatcgcg cgcggggtat cgtgcttgtc cgcatatttc atcgcgacat
     1981 cggtgccgac ctggatcggc gacgtcgcgt cgaccacgat aatcacgctg tcgaggaccg
     2041 gcacagacgc ggcgatctca cccacgaaat ccgcgtaccc gggatgatcc accaggtgga
     2101 tgcgctttcc cgcccattcc agattcatga gcttcatgca aatggtctgc tggcggtcga
     2161 tctcttcctg gaggtagtcg ccaaccgtat tgccatcctc gatcttgccc agacggttgg
     2221 tcttgccggc caggtgcagg atatgctcga tcaacatcgt cttgccggtg ctgctatgtc
     2281 ccagaatacc aacgtttcgt acatttgccg ggtcgacaat ggccattttc taaatcctcc
     2341 aaaaaacgag ttttccctga gtgcgctggc ccggtggccc gtatgactat gctgatcccc
     2401 gctaaaaggc tgccaatcga gaacggacac cgaacggcaa gtatactacg cagggaagac
     2461 gtctctcaag ctgaccacgg catctgaccg cgatgcccgg ttcccgcgtc cgccgcggca
     2521 gggcggaatc gtcactgtcc gcgctgaatg cccagaaatt cgaggctggc gcaggtgtag
     2581 gcattggtct ccagtgcgta ggtgatcgga cgccccgtcg attccagcgt cttcaatgca
     2641 tcgcggatgt tgaggcttcc ctgcccgagg ccgagatgat cgtcgtacgt gcccc
//
LOCUS       DAOPBQ010000511         2678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30047_length_2678_cov_3.171941, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000511 DAOPBQ010000000
VERSION     DAOPBQ010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30047_length_2678_cov_3.171941"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..2312)
                     /locus_tag="PKW60_13530"
     CDS             complement(210..2312)
                     /locus_tag="PKW60_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00501.1"
                     /translation="MKKRVLAWCAWVLICAPTWAVAPAAPTFQELMNPALFPEPQMGM
                     SVIASRGLDGGGVEVVTTGAVFTIHAQSGDIVCSQRIGREREVAVLRLGQPLEGVQIT
                     HSGPGFVRVTAEQPPLAMRINGDSLLMLHTATPQKVALDRRIATGWHASFENNHLLAD
                     EWGAFGLYCSDRSLDDAFDPYATTVAAYPLPADAVLWLGVCPPKPYPWDQSLEDRVVW
                     HWSRETGYPPDADLRAWKDYGNIVLLQSEVLLWKDWNLDFQPRHGPEEFARVRTTIHD
                     QGMRFIVYTSPYYFLKGTPVEPAAFNSFENFKGWPPGWPTGENMELFMDAITRVMNEL
                     QPDGLYFDGQYIGNPAALYALARRTRALLGEDGILEWHSTSALGNGHCYLPQADAYVD
                     FILRGEGRQSAYADFDYLRYFVSGYNINNCIGVLCNNGPVGVSPELVRDVLRANCRFH
                     VIVRWLDNPEVMKVLKDEYFSKLTPALRDEVDAAAEARQEGVAARAAAILAEQEALQR
                     APDWDAPAFRVEFDAMPDANAAVSPANPEALSIQDGALSIQAHGNTYAFLKIALAVQA
                     RGFVVKLRQGTDAGQSWGPGAMLVWANGSGIRAGTRSDRTLQTDVCGQQAHGGVYDPA
                     QWVWLRMRWFERLGVVERSDDGENWVRLWTFEPCQAVLGETASLLVGKVPFNGEAADH
                     REPGPEGRCDIEFVHVYP"
     gene            complement(2375..>2678)
                     /locus_tag="PKW60_13535"
     CDS             complement(2375..>2678)
                     /locus_tag="PKW60_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HPK00502.1"
                     /translation="PDVPLCWFRFLTGLPCPGCGLTRSVFAIGGGNFAAAWQSNPFGY
                     VVYGVLVVLFLSPLLARVAPRLTALLESNRFIYVFAMVNFTGLLVFGLVRLVLLWN"
BASE COUNT          519 a          903 c          787 g          469 t
ORIGIN      
        1 cttccagcgg cccacactcc acgcgccacg gcgcagtaat cgcacgtttt cgaagatacg
       61 actccactgt gctcacaacg gctcccctcg tggcattaca gaacctggcg gcaggcaatc
      121 cgtggattca cggccgcacc gtgtccgctc accggcgttt atgccgaagc gaaggaatcg
      181 gggaaagccc ggattatttt tctgccggct cacggataca cgtgcacaaa ttcgatgtcg
      241 caccggcctt cgggaccggg ttcgcggtgg tctgccgcct cgccgttgaa gggcaccttg
      301 cccacgagca ggctggccgt ttcgcccagg accgcctggc agggctcaaa cgtccagagc
      361 cggacccagt tctcgccgtc atcgcttcgc tcaacgacgc ccaggcgctc gaaccagcgc
      421 atccgcagcc agacccattg ggcgggatcg tataccccgc cgtgcgcctg ctgcccgcac
      481 acgtcggttt gcagcgtcct atccgaccgc gtaccggcgc gaatgccgct gccgttcgcc
      541 cacaccagca tcgcgcccgg tccccacgac tggccggcgt ccgttccctg ccggagtttc
      601 acgacaaagc cgcgtgcctg cactgccagg gcaatcttca ggaacgcgta tgtatttccg
      661 tgcgcctgga tggagagcgc accgtcctgg atcgacagcg cctcgggatt cgcgggcgaa
      721 accgccgcgt tggcatcggg catcgcatcg aactcgacac gaaaggccgg cgcatcccag
      781 tccggggccc gttgaagggc ctcctgttcg gccaaaatgg cggccgcgcg cgcggcaacg
      841 ccttcctgcc gcgcttctgc cgccgcatca acctcgtccc gcagcgcggg cgtcagcttc
      901 gagaaatatt cgtctttgag gaccttcatc acctccggat tatccagcca gcggacgatc
      961 acgtgaaacc ggcaattggc ccggagcacg tcgcgcacca gttcaggcga cactccgacc
     1021 gggccgttgt tgcacagcac gccgatgcag ttgttgatgt tgtagcccga cacgaaatac
     1081 cgcaggtagt cgaaatcggc ataggcgctc tgccgccctt cgccccggag gatgaaatcg
     1141 acgtaggcgt cagcctgcgg caggtagcaa tggccgttgc cgagtgcgga tgtcgaatgc
     1201 cactcgagga tgccgtcctc gccgaggagt gctcgcgtgc ggcgggccag ggcatacagc
     1261 gcggccggat tgccgatgta ttgtccgtca aaatagaggc cgtcgggctg tagctcgttc
     1321 atcacgcggg tgatcgcgtc catgaacagc tccatgtttt cgcccgtggg ccatccgggc
     1381 ggccacccct tgaaattttc gaacgaattg aaggcagccg gctctaccgg cgtgcctttc
     1441 agaaagtagt acgggctcgt gtacacgatg aaccgcatcc cctggtcatg gattgtcgtg
     1501 cgtacgcgcg cgaactcctc cggaccatgg cggggttgaa agtccagatt ccagtctttc
     1561 cacaacagca cttccgattg aagcaggacg atgttcccgt aatccttcca ggcgcgcagg
     1621 tccgcgtcgg gcggataccc cgtctctctc gaccagtgcc ataccacgcg atcctctaac
     1681 gattgatccc acggataggg ttttggcggg cacaccccca gccacagcac cgcgtccgcg
     1741 ggcagaggat acgccgctac ggtcgttgcg taggggtcga acgcatcgtc cagcgatctg
     1801 tccgagcaat agagcccgaa tgcaccccat tcgtcggcga gcaggtggtt gttctcaaaa
     1861 gacgcgtgcc atcccgtggc aattcggcga tcaagcgcca ccttctgcgg cgtggcggtg
     1921 tggagcatca gcagcgaatc gccgttgatc cgcatcgcca gcggaggctg ctccgccgtg
     1981 acgcgcacga atcccgggcc cgagtgggtg atctgcacac cttcgagcgg ctgtcccagg
     2041 cgcagcacgg ccacttcgcg ttcccggccg atgcgctggc tgcagacgat atcgcccgat
     2101 tgcgcatgta tcgtaaagac tgccccggtg gtgaccactt ccacgccgcc gccgtcgagt
     2161 cctctgctcg cgattacgct cattcccatt tgaggttccg ggaacagcgc gggattcatc
     2221 aactcctgga acgtcggcgc ggcaggcgcc acagcccagg tcggcgcgca gatgagcacc
     2281 caggcgcacc aggccagcac acgttttttc atgaatcact cctcgccggt tgcggcttct
     2341 cgaatggagc atagcaaaac gaggtacgcc gccctcagtt ccacagaaga accagccgca
     2401 ccaggccgaa aaccagcaat cccgtgaaat tcaccatcgc aaacacgtaa atgaaccggt
     2461 tactctcgag cagcgcggtc agtctgggcg cgacacgtgc cagcagcggc gaaaggaaca
     2521 aaaccaccag aacgccatag accacgtatc caaagggatt gctctgccag gccgcggcga
     2581 aattgccccc gccgatggcg aacaccgagc gcgtcagccc gcacccgggg catggtagtc
     2641 ctgtcagaaa acgaaaccag cacagcggca catccggg
//
LOCUS       DAOPBQ010000512         2673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30122_length_2673_cov_3.739878, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000512 DAOPBQ010000000
VERSION     DAOPBQ010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30122_length_2673_cov_3.739878"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..2495)
                     /locus_tag="PKW60_13540"
     CDS             complement(159..2495)
                     /locus_tag="PKW60_13540"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPK00503.1"
                     /translation="MLRAVIIGLLLAVAVPSGAMTIAEDGHARAAIILCDSPEIPEQT
                     AARELAAYLNSVTGGNFTVIPESQAPADGPRVFVGPTQFAASQGIDFTSVGPEEWVIR
                     TVGRDDLILAGGRPRGTLYAVYHFLEDVLGVHWWNAWEETVPQNSTLRLGPLDHTGKP
                     AFRSRDIYMLYGNDEGRFAARNRLNRDGDTPISPDYGGAMDYGPPYHVHTFYMYVPPE
                     SYLTEHPEWYSLVKGQREAGYRQLCLTNPDLRTFFVEKLKSYIEQSRAEARASNAPAP
                     TVFSISQNDWEGPCECSDCQAIAEAEESEAGPLLDFVNYVADAIREDYPEVYIDTLAY
                     MMTQKPPKTIRPRDNVIIRLCDTSSNFTKTIQDPLNQEFHDHLLRWAAIARNLRIWDY
                     AVTYAPHYGLPMPTAHTYGPDYQFYAENNVEGVFTEHEYPVLADMRDFKVWMMMKMLE
                     DPCQDYSGLVQTFTDGFYGAAGEFIRQYLTRLEQASEAKPSYLSMGASPRQYHYLDLP
                     FVLDAHERFDQAEQAVAGNDALLRRVRFARLPLDRASLVLFPDLAAQWVRGGNTPETM
                     PLDREAIAARCRDTWTTQVYFRIPGEERPAALAEIDAELDPLLVRPAIVHIPEKFRDL
                     PPETVFDFTADMTRNWNDEARRVPDPQAESGLTNRLELTGDDKARYALPMPWGLYDVI
                     NKRGRGDGSIRAEDVPGPGYHWYKMGTFEIQPSFYVYFFWSWIIQVDVDWAHDPAHPE
                     QRFDIWARIKFEGPTFPHGDPEAKDAISIERVVLVKMP"
BASE COUNT          532 a          771 c          833 g          537 t
ORIGIN      
        1 ttgatacgga tatttcagtg ttttcaatcg gttgccagcg gtttcatact atacccccgc
       61 ccgcccccgg cgtccaagtc acggcagtgg acaggacaga aaaggcacgc cgctcacaga
      121 ttttctggcg cgcatcgcgt cgtttgtatg cggcgttctc agggcatctt gacaagtacc
      181 acacgttcga tggaaatggc gtctttggcc tcagggtcac cgtgcggaaa agtgggtccc
      241 tcaaatttga tacgcgccca gatatcgaaa cgctgttcgg gatgcgcggg atcgtgcgcc
      301 cagtcgacat cgacctggat gatccagctc cagaaaaagt agacgtagaa cgagggctga
      361 atctcaaagg ttcccatctt gtaccagtgg tacccgggcc cgggcacgtc ttcggcacga
      421 atacttccgt cccccctgcc ccgtttgttg atgacgtcgt acaatcccca gggcatgggc
      481 agagcgtagc gcgccttgtc atcgccggtc agctccaggc ggttcgtcag gccggattcg
      541 gcctggggat ccggaacccg tctggcctcg tcgttccaat tgcgcgtcat atcggcagtg
      601 aaatcgaaca ccgtttcagg gggcagatca cggaatttct cgggaatatg cacaattgcc
      661 gggcgtacaa gcagcggatc gagctccgca tcgatttcgg ccagtgctgc cgggcgttcc
      721 tcccccggga tgcggaaata aacctgcgtg gtccaggtgt cccggcaacg agcggcgatt
      781 gcctcgcgat cgagcggcat cgtctcggga gtatttccgc cgcgcaccca ctgcgctgca
      841 aggtctggaa agagcaccag ggaggcgcga tccagcggca gtcgcgcaaa ccggacccgg
      901 cgcagcaggg catcgttacc ggccacggcc tgctccgcct ggtcgaaacg ctcgtgggcg
      961 tcgagcacga acggaagatc gagataatgg tattgccggg gagatgcgcc catgcttaag
     1021 tagctcggct tggcctcgga ggcctgttcg agccgggtca ggtactgccg gatgaactcg
     1081 ccggccgccc cgtaaaagcc gtcggtgaag gtctggacga ggccgctgta gtcctgacag
     1141 ggatcttcaa gcattttcat catcatccag actttgaaat cgcgcatgtc ggccagcacg
     1201 ggatactcgt gctcagtaaa gacgccttcg acgttgtttt cggcgtaaaa ctggtaatcg
     1261 gggccgtagg tgtgcgcggt gggcatgggt aggccgtaat gaggggcata ggtgactgca
     1321 tagtcccaaa tccgcagatt tcgagcgatg gccgcccagc ggagcaggtg atcgtggaat
     1381 tcctggttga gcgggtcctg aatcgtcttg gtgaaattgc tggaggtgtc gcagaggcgg
     1441 atgatcacgt tatcgcgggg ccggatggtc ttggggggct tctgggtcat catataggcg
     1501 agggtatcaa tataaacctc gggatagtct tcccggattg catcggcgac gtagttgaca
     1561 aaatcgagca gcggccctgc ttccgactcc tcagcctccg cgatggcctg gcaatcgctg
     1621 cactcgcacg ggccttccca gtcgttctgg gaaatgctga agacggttgg cgcgggcgca
     1681 ttgcttgcgc gagcctccgc acgggattgt tcaatataac tcttcagttt ttcgacgaaa
     1741 aacgtccgca gatcgggatt cgtcaggcaa agttgcctgt atccggcctc gcgctgtccc
     1801 ttgaccagcg aataccattc ggggtgctcg gtgaggtacg attccggcgg aacatacatg
     1861 tagaacgtgt gcacatggta gggcggcccg taatccatcg ctccgccgta gtcgggggag
     1921 atcggagtat caccgtcgcg gttcagccgg ttccgggcgg cgaagcgtcc ctcatcattc
     1981 ccgtagagca tgtagatatc acggctgcgg aaggcgggct tgccggtatg gtcgagaggg
     2041 cccagccgca gggtgctgtt ctggggtacc gtctcctccc aggcgttcca ccagtgcacg
     2101 ccgagaacgt cttcgaggaa atggtaaacg gcgtagagcg ttccgcgggg ccgtcctccc
     2161 gcgagaatga ggtcgtcgcg gccaacggtc cgtatgaccc attcctcggg cccgacggag
     2221 gtgaaatcga tgccctggga tgcggcaaac tgcgtcggcc cgacaaacac gcgcggtccg
     2281 tccgcgggcg cctgcgattc cgggataaca gtaaagtttc cgccggtcac cgagttcaga
     2341 tacgccgcaa gctcgcgcgc ggccgtctgc tcagggatct cgggagaatc gcagagaatt
     2401 atcgcggcac gggcgtgacc atcctcggca atcgtcatcg cgcccgaggg cacggcgaca
     2461 gcaagcagca atccaataat cacagcacgc aacatgtcta atcctccgtc gatgtgtata
     2521 aactcagcgc ccaggcctgt acacaggccg ggcaggccgg ccgcaccgca gcggtttcgc
     2581 gtcaatttat gagcgagtgt accgaaattc ccgacgaagc gcacttctgc ccatgatcct
     2641 gagttgatac cctgccggaa agaagcgtag att
//
LOCUS       DAOPBQ010000513         2665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30235_length_2665_cov_3.719540, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000513 DAOPBQ010000000
VERSION     DAOPBQ010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30235_length_2665_cov_3.719540"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKW60_13545"
     CDS             <1..245
                     /locus_tag="PKW60_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00504.1"
                     /translation="SRWAKDGGQLALEVVVPANCRAQIAVPVSGNRNPVITESGKVIW
                     ENGQYVAGQDGITAGQEDGEWRVFTAGSGSYRFICR"
     gene            complement(398..1441)
                     /locus_tag="PKW60_13550"
     CDS             complement(398..1441)
                     /locus_tag="PKW60_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HPK00505.1"
                     /translation="MSTHITEDSPLRTTSPRTNPARRAAGFTLIELLVVIAIIGILAA
                     ILLPALARAREAARRASCQNNLKQMGLVFKMYANESGGLFPRIHGDQPWGAALPAGCT
                     SGNIRASLAPQIAAIFPEYLSDLNVLICPSDPEGSSGNPLALIDHLPGQTCLYQGLPS
                     KPDCSYLYYSFLIDKVSETDPAFDVAMFGFPSALVSAQLAYLMACLSHLPPTFNGVLG
                     DRDPGNDVLLDLDINNAAVHGMFAAMAMPPGASLGNGGGATLYRIREGGERFVITDIN
                     NPAASATAQSTMPIMWDVVSATTTASAQFNHIPGGANTLYMDGHVAFNRYPGEFPASR
                     SFAVLGALFSLGE"
     gene            1742..>2665
                     /locus_tag="PKW60_13555"
     CDS             1742..>2665
                     /locus_tag="PKW60_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPK00506.1"
                     /translation="MDAKASGSRTTRRAFVKSAGALGASLYLAGGGSKRTVLAARGKK
                     ETLALNGGPKAVTTAAGDSTKWPLYGEEEIAAVTELLRSPGYGPVAEFEEAWKAYHGC
                     PYVKAHCNGTSALTSMLFALDLPAGSEVLVPNYSTWFPVVPMRFFDLVPVFVDADPRT
                     MNLSLEDCKKRLTGKTKAIMPVHWYGLPCEMDEIMAFAAEHGLEVVEDASHSHGARIQ
                     GKLTGTWGRMAGISLQGTKPLPSIEGGIANYKEQRDYERATTYGNYDLPNTFPEDSPY
                     RKYQGTAFGSKLRIHPVSAILARIQLKQMDAR"
BASE COUNT          519 a          727 c          917 g          502 t
ORIGIN      
        1 tctcgcgctg ggcgaaggat ggcgggcaat tggccctcga ggtggtggtg ccggcgaatt
       61 gccgcgcaca gatcgccgtg cccgtatcgg gcaaccgcaa cccagtgatc acggaaagcg
      121 gaaaggtgat ctgggagaac ggacagtacg ttgccggcca ggatggcatt accgctggcc
      181 aggaagatgg cgagtggcgg gtgttcacgg caggttcggg gtcgtaccgg ttcatctgcc
      241 gttaatcaga aacaaatgcg taaacgaagg cgacgtacaa gcgtcgcctt cgttgttttt
      301 ctaccggctg cagtggatct cgctgtgact gattgacggt ttgcggcatt tcacctgcag
      361 cgcgccgtct ggccgtactt atttccttgt tcctggttca ttcccccaga ctgaacaggg
      421 cgcccagaac ggcaaacgac cgggacgcgg gaaattctcc gggataccgg ttgaacgcca
      481 cgtggccgtc catgtaaagc gtgttggctc caccgggaat atggttgaac tgggcgcttg
      541 cggtggtggt ggccgatacg acgtcccaca tgatcggcat ggtgctttgc gcggtggctg
      601 aagctgccgg gttattgatg tcggtgatga caaatcgttc gcccccttca cgaatccggt
      661 aaagcgttgc accgcctccg tttccaagac ttgcacccgg cggcatcgcc atggcggcga
      721 acatgccgtg caccgcggcg ttgttgatgt ccaggtccag caagacatcg ttgccggggt
      781 cgcggtcacc caagacgcca ttgaaggtgg gcggcaggtg cgacaggcaa gccatcagat
      841 aggccaactg tgcggaaacc agggcggagg gaaagccgaa catggcgaca tcgaacgccg
      901 ggtcggtctc ggagaccttg tcgatcagga agctgtagta gaggtagctg caatcgggct
      961 tggaggggag gccctgatac agacatgtct ggccgggcag gtggtcgatg agggcaagcg
     1021 ggttgccgct gctgccttcg gggtcggatg ggcagatgag cacgttgagg tcggagaggt
     1081 attcggggaa aatggcggcg atctgcgggg ccaggctggc tcgaatgttg ccggaggtgc
     1141 atccggccgg caaggccgca ccccagggct gatcgccgtg gatgcgcgga aagagaccgc
     1201 ccgattcgtt tgcatacatc ttaaacacga gacccatctg cttgagattg ttctggcaac
     1261 tcgcgcggcg ggcggcttcg cgggcgcgtg cgagggcggg cagaaggatc gcggccagaa
     1321 tgccgataat ggcgatgacc accagcaatt caatgagtgt gaatccggca gcccggcggg
     1381 cagggttagt tcgtggcgat gtcgtgcgca atggactgtc ctccgtgatg tgtgtactca
     1441 tccgtcgaca aggacggatg cgccgcgtaa aggccgccgc cctggaattc ctggagcgcc
     1501 cccgcacggc aggatccttt ggggtcggga ctttgacgag gctccctgtg ccgctccagg
     1561 ggcccttcgc aggcattcca tatgccatcg gcgaaggcga ccagcccctg gcggactcct
     1621 ttgagtttcc catatcagcc tgcctgttgt aaaatcgcgc actggttgat tctccgccgg
     1681 aattgcgcca tgatgtgggc gagactgggt gacggatccg aaatgatggg aggtatgaga
     1741 gatggatgct aaagcgagcg gttcccggac gacgcgccgg gcgtttgtga agtcggcggg
     1801 cgcgctgggc gccagcctgt atctcgcggg gggaggatcg aagcgtaccg tcctggcagc
     1861 gcggggcaag aaagaaacgc tggcgttgaa cgggggcccg aaagcggtta cgacagctgc
     1921 cggcgattcg acgaaatggc ccctctacgg ggaagaagag attgcggcgg tcaccgagtt
     1981 gttgcgttct ccgggctatg ggcccgtggc ggagtttgag gaagcctgga aagcctacca
     2041 tggctgtcct tatgtgaaag cgcactgcaa tggaacgagc gcgctgacct cgatgctgtt
     2101 tgcgctggat cttcccgccg gcagcgaagt gctggtgccc aactacagca cgtggttccc
     2161 ggtggtgccg atgcgctttt tcgacctggt ccctgtattc gtggacgccg atccccggac
     2221 gatgaatctg tcgcttgaag actgcaagaa gcggctgacg ggcaagacga aggcgatcat
     2281 gccggtccac tggtatggcc ttccgtgcga gatggacgag atcatggcgt ttgcagccga
     2341 acatgggctg gaagttgttg aggatgccag ccactcgcat ggcgcgcgga tccaaggcaa
     2401 gctgacgggc acctggggcc gcatggcggg catcagcctg caggggacga agccgctgcc
     2461 gtcgattgag ggcggaatcg ccaactataa ggaacagcgg gattacgagc gggcgaccac
     2521 ctatggcaac tacgatctgc ccaacacgtt cccggaggac agcccgtacc gcaagtacca
     2581 ggggacggcg tttggctcaa aactgcggat ccacccggtg tcggccatcc tcgcccgcat
     2641 ccagctcaag caaatggatg cgcgg
//
LOCUS       DAOPBQ010000514         2665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30237_length_2665_cov_3.373946, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000514 DAOPBQ010000000
VERSION     DAOPBQ010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30237_length_2665_cov_3.373946"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..907)
                     /locus_tag="PKW60_13560"
     CDS             complement(92..907)
                     /locus_tag="PKW60_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPK00507.1"
                     /translation="MHAHHLARVPLLLSLAFCVSAAELTFEDVAFTARCDGSEQRYVL
                     GLPATQETGQPRDALIALHGHGSDRWQFVREERGECAAPRHIAQERGMIFISPDYRAK
                     TSWMGPKAEADLLQIMEEVREKYDVARFFICGGSMGASSALTFAALHPDLFAGVAAMN
                     GTANHLEYENYQDAIQESFGGTKAEIPEEYKKRSAEYWPERFTMPLAMSLSGQDKAVP
                     PESSLRLADVLQKMDRKVLVIFREEQGHFSTYEDTRALVEYILAQADAIASEP"
     gene            complement(1040..>2665)
                     /locus_tag="PKW60_13565"
     CDS             complement(1040..>2665)
                     /locus_tag="PKW60_13565"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPK00508.1"
                     /translation="PFATLHRAQEAVRGQIAQGLDNNVTVLIRGGEYVLEEPLVFGPA
                     DSGNDNFIARYAAYPGETPVLSGGRRLTGWTQGENNRWTLDASGAAGGSWPFRQLFAD
                     GERLPRGRFPNPPDLLRVASVSPDVTEITLETAPPVADLAGKDAELVMYQNWSISRTA
                     IVSSDNNVLKLANPMGWIGHGDATTASPGKPAIIENALQFVDQPGEWYLDRASGLLTY
                     QAAEGEDPNSKTFIAPKLEQLILVEGQPENPVVNLRFEGLTFAHTEWTLPPFGYQGIQ
                     AGHYGTTITDRTYVLPVAIEFTYAERCALERCRLEHFGGSAIGFGQRCRYNRVAGCIL
                     TDIGGNGIMTGWRGKGRLMPTAPGEDHYLAADWADPLDVPIGNEIVANELQTCGAVNH
                     GCVAIFDAFCGGTRIAHNVVHAMPYTGISVGFRWDESPTSQRGAVIEYNHVYDTMRML
                     ADGGCLYTLGWQPDAVIRGNVFHDAHRSEFAHGGAPNNGIFFDQGSKGFLVSGNTIYR
                     CSGGPIRFNQTGTANMAWEDNAFEIGPGYATEP"
BASE COUNT          492 a          793 c          854 g          526 t
ORIGIN      
        1 tactgccatg gacggttcct ttcatcggtg cccccggctg ctgcggctgc ctgcccgggg
       61 aatatttcaa caacgttcgg cgcagcggag attacggctc actcgcgatg gcatcggcct
      121 gagccaggat gtattcaacg agggcccggg tgtcctcgta ggtgctgaaa tggccctgct
      181 cttcccgaaa gataaccagc accttgcgat ccatcttctg aagcacatcc gcaagccgga
      241 ggctgctctc cggcgggacc gccttatcct ggccactgag cgacatggcg agcggcatgg
      301 tgaagcgttc aggccagtat tccgcgctgc gcttcttata ctcctccgga atctccgctt
      361 ttgtcccccc aaatgattcc tggatcgcat cctggtaatt ctcgtattcg aggtggttgg
      421 ccgtgccgtt cattgcggcg acgcctgcga acaggtctgg atgcagggcc gcgaacgtga
      481 gagcgctgga cgcgcccatc gagcctccac agataaagaa acgcgcaaca tcatactttt
      541 cccgcacttc ctccatgatc tgcagcaggt cggcttcggc cttcggaccc atccaggagg
      601 tcttcgcgcg gtaatccggc gagatgaaga tcatgccgcg ttcctgagcg atgtgacgcg
      661 gggccgcgca ttctccgcgc tcttcccgca cgaattgcca gcgatccgag ccatggccat
      721 ggagggcgat cagggcatcg cgaggctggc cagtctcctg agtcgccggc aggccgagga
      781 catagcgttg ctcggagcca tcacagcgcg ctgtgaacgc cacgtcttca aacgtaagct
      841 ccgcggcgct tacacagaat gccagggaga ggagcagcgg cacgcgagcc aggtgatggg
      901 catgcattgg aatcgaaccc tttctgccgt gggagtgtct ggaatgcaag aatactcctt
      961 gcgaacgcgc cgacgcaaaa cgcgagtgcc ggcgccctca aagtaagggc ggtcgcggcg
     1021 aagagcacgg aggggaatct cacggttcag tggcgtaacc ggggccgatt tcaaaagcgt
     1081 tgtcttccca ggccatgttc gcggtgccgg tctggttgaa gcggatgggc ccgccggagc
     1141 aacggtagat cgtgttgccg ctgaccagga accccttact gccctgatcg aagaagatgc
     1201 cgttattcgg ggcgccgcca tgtgcgaact cgctgcggtg ggcatcatgg aaaacgttgc
     1261 cgcgaattac tgcgtccggc tgccatccca gagtgtacag gcatccgcca tcggccagca
     1321 tgcgcatggt gtcgtagacg tggttgtatt cgatgacagc gccgcgctgg ctggtggggg
     1381 attcgtccca gcgaaatccg accgagatgc cggtgtaggg cattgcgtgc accacgttat
     1441 gggcgatacg ggtccccccg cagaaggcgt caaaaatcgc aacacagcca tggttgacgg
     1501 cgccgcaggt ctgaagttca ttggcaacga tctcgttgcc gatgggcaca tccagcggat
     1561 cggcccaatc cgccgcgagg taatggtctt ccccaggcgc cgtaggcatc agccggcctt
     1621 tgccgcgcca gccggtcatg atgccgttgc cgccaatatc ggtgagaatg cacccggcga
     1681 cgcgattgta ccggcaacgc tgaccgaatc cgatggctga gcctccgaaa tgctccaggc
     1741 ggcagcgttc aagcgcgcac ctctctgcgt acgtgaattc gatggcgacg ggaaggacat
     1801 aggtgcggtc cgtgatggtg gtgccgtaat gtccggcctg gatgccctga tagccgaacg
     1861 gggggagggt ccattcggta tgggcaaagg tcaggccctc gaagcgcaga ttgacgacgg
     1921 ggttttccgg ctggccttcg accagaatca gttgttcgag ttttggtgcg atgaaggtct
     1981 tcgagttggg atcttcaccc tcggccgcct ggtaggtgag caatcctgag gcgcggtcca
     2041 ggtaccattc gccgggctgg tcgacgaatt gcagcgcgtt ctcgataatg gccggtttac
     2101 ccggactggc tgtagtcgcg tcgccgtggc cgatccatcc cattggattg gccagtttga
     2161 gcacgttgtt gtcggaggag acgatcgcgg tccgtgagat tgaccagttc tggtacatga
     2221 ccagctccgc gtcttttccg gcgagatcgg caaccggcgg ggcggtctca agggtgatct
     2281 cggttacatc cggggagact gacgctacac ggaggaggtc cggcgggttt ggaaagcgcc
     2341 cgcgcgggag gcgttctccg tcggcgaata gctgccggaa aggccaactg cctccggctg
     2401 ctcccgaggc gtcgagcgtc caccggttgt tttcgccttg cgtccagccc gtgagacggc
     2461 gtccacccga cagaaccggc gtctcgccgg ggtatgccgc atagcgggcg atgaaattat
     2521 cgttgccgga gtccgctgga ccaaaaacca gtggctcttc caggacgtat tcgccccccc
     2581 ggatgagcac ggtcacattg ttgtccaagc cctgagcgat ctggccgcgt accgcctcct
     2641 gcgcgcggtg aagcgtcgca aacgg
//
LOCUS       DAOPBQ010000515         2664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30248_length_2664_cov_4.507857, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000515 DAOPBQ010000000
VERSION     DAOPBQ010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30248_length_2664_cov_4.507857"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKW60_13570"
     CDS             complement(<1..391)
                     /locus_tag="PKW60_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00509.1"
                     /translation="MSEISCPHCGKTRIMATRIPRDVVMMLPCPECHELVVLFRGKAI
                     PLNRDVLRNGTKEERRNHIAEIVEECLPPELFSDAVHWNFTDIGSPFTFGDRKSRRRR
                     RHTEKEEDGRDRPITDEEVARFRKFELH"
     gene            complement(536..>2664)
                     /locus_tag="PKW60_13575"
     CDS             complement(536..>2664)
                     /locus_tag="PKW60_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPK00510.1"
                     /translation="LKGTGRSVAAEAAQLRSAMAEVQINCPKCGAVYPSGHQMCVRCG
                     TRLPGASGTPGTGGSAGKSTSSTFRRGQVVANRYTVVDMIGRGGMGCIYKVRDNTLDE
                     DVALKTLLPKFTNDRHVVDRFFNEARIARQLSHPNIIRVHDIGMAGTVVYISMEYVQG
                     RSLREWMESIPPGQHIPISYALRLMIQLSAALEFAHKFTVHRDLKPENVMITPDGRVK
                     LMDFGISKLKSNVQLTSAQMVMGTPKYMSPEQLKDSSSVDHRSDIYALGAMLYELLTG
                     HVPVGLARQPSEIRPEITPALDALVTKCLAYEPGDRYQTAAELRVELQRLLDSVESTL
                     GIEVSELDLPVVPAARGASLFRRIAGVLLVSLVLAAGAFGVWQLERFESGEPADAGAG
                     AASALDPPADLFTQAFGELTLRSIRAREKAVATQDTALFVSEGDAFWEQALALADQRK
                     ADAFRTGWFALECYLGPMLAPLGMVFIPPGETVVERGTDPVFVSGFFIDRYEVTNGQF
                     RAFALDAGTHWPMPDYLGGARLTEDVLLSPVVGVTFYDALAYAAYTHPASWGTCTLPT
                     EAQWARAAYGDARTPRPFPWGPEWDPSAASAAGEADGFAGAAPVGYFGPDTSLFGCRD
                     LAGNVMEWTRSVFTYPYDASVAEQELETIWFGSALTVRGGCYTDEQASMAERRSAWFE
                     ESSPLIGFRCVLPFPTESETIDAFLR"
BASE COUNT          494 a          862 c          803 g          505 t
ORIGIN      
        1 ggtgcagctc gaatttgcgg aaccgcgcca cctcttcatc cgtgatcggc cgatcccgtc
       61 cgtcctcctc tttctcggta tggcgcctgc ggcggcggct cttgcggtct ccaaaggtga
      121 acggcgagcc gatgtcggtg aaattccaat gcaccgcgtc cgagaagagt tccggaggca
      181 ggcattcctc cacgatttcg gcgatgtggt tgcgccgctc ttctttcgtg ccgttgcgca
      241 aaacgtcacg gttcagggga atggctttgc cccggaagag cacgaccagt tcgtggcatt
      301 cagggcatgg aagcatcatc accacatccc gcgggatccg ggttgccatg atgcgcgtct
      361 tgccgcagtg ggggcagctg atttcactca tgtggctctc ttttgaggtc aatgagggtt
      421 aaacgccggt atgcccgtat tgtatgcctg ccgggatggc cctgtcaaac cgccgaacgc
      481 tgaattggat gaaaaaacgc ggaaaaaccc gcttttctgg ccaaataacg ggcattcagc
      541 ggaggaatgc gtcaatggtc tccgattccg tggggaacgg cagcacgcac cggaacccaa
      601 tcagcgggct ggattcttcg aaccaggcgc ttcggcgctc cgccatcgag gcctgctcgt
      661 cggtgtaaca gccgccgcgt accgtcaggg ccgacccaaa ccagatcgtt tcgagctcct
      721 gttcggccac ggacgcatcg tagggatacg tgaatacgct gcgagtccat tccatcacgt
      781 tgcccgcgag gtcgcggcag ccgaacaatg aggtgtccgg cccaaaataa cccaccggag
      841 cggcgcctgc aaagccatcg gcctcgccgg ccgcgctcgc cgcgctcggg tcccattccg
      901 gcccccaggg aaacggccgg ggcgttcggg catcgccata ggccgcacgg gcccactggg
      961 cctctgtcgg cagcgtgcac gtgccccacg acgcgggatg agtatacgcc gcatacgcga
     1021 gcgcgtcgta aaacgtgacc cccaccacag gggacaggag cacatcttcg gtaagccgcg
     1081 ccccgcccag atagtcgggc atgggccagt gcgtcccggc atcaagcgcg aacgcccgga
     1141 attgtccgtt ggtgacctcg taccggtcga taaagaaccc gctgacaaat accgggtcag
     1201 tgccccgttc tacgacggtc tctccgggcg gtatgaacac catgcccagg ggagcgagca
     1261 tgggtccgag gtagcactcc agggcaaacc atcccgtgcg aaacgcatcc gcctttcgct
     1321 ggtctgcaag cgccagggct tgttcccaga aggcatcgcc ctcagagacg aagagcgccg
     1381 tgtcctgcgt ggccacggcc ttctcacgcg cacggatcga ccgcagggtc agctcgccaa
     1441 acgcctgcgt gaacaggtcc gccggggggt ccagtgcgga cgccgcgcct gccccggcat
     1501 ccgccggctc tccggattca aaccgttcca gttgccagac accaaacgcc ccagccgcca
     1561 acacgaggga tacgagcaga acgcccgcga ttctccggaa aagcgatgcg cctcgcgccg
     1621 ccggcactac cggcagatca agctcggaga cctcaatgcc tagagtgctc tctactgaat
     1681 cgagcaagcg ctgcagctcc acgcgcagct ctgcggcggt ctggtaccgg tcgccgggtt
     1741 cataggccag gcatttcgtg accagcgcgt cgagtgcggg cgtgatttcc gggcgaatct
     1801 cagagggttg acgcgccagc cccaccggca cgtggcccgt cagcagctcg tacagcatgg
     1861 cgcccagcgc gtaaatatcg gaccggtgat ccacgctgct gctgtctttc aattgctcgg
     1921 gcgacatgta tttcggcgtg cccatcacca tctgcgccga cgtcagctgc acattggatt
     1981 tcagcttcga gatcccgaaa tccatcagtt tgactcggcc atcgggcgtg atcatcacgt
     2041 tttcgggctt caaatcgcgg tgtacggtga acttatgggc aaactccagt gctgcgctca
     2101 gctggatcat cagccgcagg gcatatgaga tgggtatgtg ctgtcccggc gggatcgatt
     2161 ccatccattc acgcagcgaa cggccctgca catactccat cgaaatgtag acgacggtcc
     2221 ccgccatgcc gatgtcgtgc acgcggatga tattggggtg ggaaagctgc cgcgcgatgc
     2281 gtgcttcatt gaaaaaccgg tcgaccacgt gccggtcgtt ggtaaatttc ggaagcaggg
     2341 tcttgagggc cacatcttcg tccagcgtgt tatcgcgcac cttgtagatg cagcccatgc
     2401 cgccgcgtcc gatcatgtcg actacggtgt agcggttggc cacgacctgg ccccggcgaa
     2461 acgtcgagct tgtgcttttg ccggcgcttc ctcctgtgcc cggggtcccg ctcgcaccgg
     2521 gaagccgtgt gccacagcgg acacacatct ggtggcccga tgggtacacc gcgccgcatt
     2581 tcgggcaatt gatctgcact tctgccatgg cgctccgtag ctgtgccgct tccgcggcca
     2641 cgcttctgcc ggttcccttc aaat
//
LOCUS       DAOPBQ010000516         2663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30272_length_2663_cov_2.837040, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000516 DAOPBQ010000000
VERSION     DAOPBQ010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30272_length_2663_cov_2.837040"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..838
                     /locus_tag="PKW60_13580"
     CDS             170..838
                     /locus_tag="PKW60_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HPK00511.1"
                     /translation="MRRILSRFTGAPGSALVLVLLAVMPGCQKAAPPEQPGREEAATP
                     QLPGPESRPTEIETAQSGQEGAITMKLTINSPAFADQGTIPRKYTGDGEDVSPPLAWT
                     GAPAETRSFALICDDPDAPVGTWVHWVLWNIPATETGLAEAIPADRPELPNGAIQGVN
                     DFRKTGYGGPAPPRGPVHRYFFKLYALDRFLDLAPGAGKDALEMAMSEHILASTEMVG
                     RYGR"
     gene            1006..1941
                     /locus_tag="PKW60_13585"
     CDS             1006..1941
                     /locus_tag="PKW60_13585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPK00512.1"
                     /translation="MATKIAKSEYVPGEYIAKRYEVKRAIGKGGMGMVYLVVDHKTGE
                     PMALKTIRPEYTTSSYALHRFGREVRTIRKLNHPGIVRIRDAFRYGRLVFYTMDYVEG
                     QSVRELIQQRRRLGLGSTVRILALVAHALEHAHNCTIHRDMSPENIMVLSDGSIRLLD
                     FGLAKLTDTEPTFTRIGVTLGKHQYISPEQLLSSAEVDCRTDIYALGIIFHEMLTGRL
                     PEKEKAITDWVPTLPASCNAFFEKATARNPDDRFATAEEFRGAIIDLFRAYRRDQDEP
                     VAAVASPSCASRPGLWARLRSWLRRWWRREKKVTA"
     gene            2290..>2663
                     /locus_tag="PKW60_13590"
     CDS             2290..>2663
                     /locus_tag="PKW60_13590"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPK00513.1"
                     /translation="MSYRASMKLFVLALVIVAVAVGGCGRKKTQITTDLTSDATAGQS
                     TGPETTTGAGLPNLEQEKLFFQQLAEAGTVYFAYDRSQLDPTAMETLKRNAEIIKQMP
                     NSVIQIEGHCDERGTQQYNLALG"
BASE COUNT          599 a          776 c          791 g          497 t
ORIGIN      
        1 atcttcctcc agaaacgaaa aatcgcgttg cgtgcactca gcacttgaat gtgagaacgc
       61 gtagtgcgcg caaaatccgt tcatggtgta gtatggaggc ggtgcagaaa ggcttgcttt
      121 aacagaaact ggggccggtc tccggcaagg aatccaaagg aggcccatga tgagacggat
      181 actgagtcga ttcactggtg cgccgggcag cgcgctcgtc ctggttttgc tggcggttat
      241 gccgggatgc cagaaagccg cgccgccgga gcagccgggc cgcgaggaag cggcaactcc
      301 gcagcttccc ggcccggaaa gccgtccaac ggagatagag accgctcaat ccggacagga
      361 aggagccatt accatgaaac tcaccatcaa cagtcccgct tttgccgatc aaggcaccat
      421 tcccaggaaa tatacgggag atggggaaga cgtgtcgccg ccgctcgcct ggacaggcgc
      481 ccctgcggag acacgctcgt ttgccctgat ctgcgacgac ccggacgcgc ccgtcggcac
      541 gtgggtccat tgggtgcttt ggaacatccc tgccactgag acggggcttg ccgaagccat
      601 cccggcggac cgccccgaac tgcccaacgg cgccatccag ggcgtgaacg atttcaggaa
      661 gaccgggtat ggcggccctg ccccgcctcg cgggcccgtc catcggtatt ttttcaagtt
      721 gtacgccttg gacagattcc tggatctcgc tcccggagca ggcaaggatg ctctcgaaat
      781 ggcaatgagc gagcacatcc ttgcgtcaac cgaaatggtg ggccgctacg gccggtgact
      841 cgggtagagg atggaaatcg cgctgaattg gcggaataca ggcctccgcc acggatgcgt
      901 tgtgcggaaa tcttgttttc tgcgcaaaac cccctctttt ctgaaccgtg aaagatgtgt
      961 tacagtacgg tcttggagac gattgggctg aaatacacaa acaccatggc tacaaaaatt
     1021 gctaaaagcg agtacgttcc tggcgagtac atcgcaaagc gctacgaagt caagagagca
     1081 atcggcaagg gcggcatggg gatggtctac ctggtcgtag accacaaaac cggcgagccc
     1141 atggcactga agacgattcg ccctgaatac acgaccagca gctatgccct gcaccggttt
     1201 ggccgcgaag tccgcacgat ccgcaaatta aatcaccccg gaattgtccg cattcgcgac
     1261 gcttttcgat acggaagact ggtgttctac accatggact acgtcgaggg ccagagtgtc
     1321 cgggagctta tccaacagcg gcggcgcctc ggactgggct cgaccgtccg tatcttggcg
     1381 ctggtggccc acgccctgga acatgcccac aactgcacca ttcacaggga tatgtcgccg
     1441 gaaaatatca tggtactttc ggatggctcg atccgccttc ttgatttcgg cctcgcaaaa
     1501 ctcaccgata ccgagccgac atttacgcgg attggcgtta cgctgggaaa acatcagtac
     1561 atttcgcccg aacagctcct cagttccgcg gaagttgatt gccgcaccga catttacgcc
     1621 ctgggcatca ttttccatga aatgctgacg ggacggctgc ccgagaaaga aaaggcgatc
     1681 acggattggg tgcccacgct tccggcgagc tgcaacgcct ttttcgaaaa agccacggcc
     1741 cgaaatccag acgaccggtt cgcgaccgcg gaggagttcc gtggagccat tatcgacttg
     1801 ttcagggcct accggcgtga ccaggacgaa ccggtggcag cggtggcgtc tccttcgtgt
     1861 gcttcgcgcc cgggcctctg ggcacgcctg agatcgtggc tgaggcgctg gtggcggcga
     1921 gaaaagaagg tcactgcctg aactcccgcc gcggacgatt tgccggtcag gaaggaacgg
     1981 gcgggttatc ggagcgtggc tggtgcacag gcccgcacgg actgcgcgct gaattttctg
     2041 cgtggggcgc cgcccggatc cctgccgatc tcaggcccgt tgtaccggcc gggaacggcg
     2101 aaaaaactgc ggcaggacat gcatttggcg cagcacagtt tgcgtgaaac ttttgtacgt
     2161 ttgacgatat tcacattgca acagcaaaac gcatttgttg acccgaagat ttgagtctgc
     2221 tacgatcgcc gcggccgaac tgttcgggta aaccacaacc acgtcaggtg gagcgaggaa
     2281 gaagaagaca tgtcgtacag agcatcgatg aaactgtttg tgttggccct ggtcatcgtc
     2341 gcggttgcgg tcggcggttg cggcagaaaa aagacccaaa tcaccaccga ccttaccagc
     2401 gatgccaccg cgggccagag caccggtccc gagaccacta ccggagcggg actgcccaat
     2461 cttgaacaag agaagctgtt cttccagcag ctggccgaag ccggcaccgt ctatttcgcc
     2521 tatgaccgtt cgcaactgga ccccaccgcc atggaaacgc tgaaacgcaa tgcggagatc
     2581 atcaagcaga tgcccaactc cgtgattcag atcgagggcc attgcgacga acgcggcacg
     2641 cagcagtaca acctggcact ggg
//
LOCUS       DAOPBQ010000517         2659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30326_length_2659_cov_3.324885, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000517 DAOPBQ010000000
VERSION     DAOPBQ010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30326_length_2659_cov_3.324885"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PKW60_13595"
     CDS             complement(<1..563)
                     /locus_tag="PKW60_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00514.1"
                     /translation="MSSRETIEHWLHRLIRTYEEAPVPHHEAGRHAKAICALRIFLGD
                     HMDDELQLKRVQCALEELEPLAMDPFQFRASVDKYIADRGDACRQFIAQGTCETLLAY
                     WATIDPSGKAAYLVLPESVWARALLKTMGLFFPCVNHAPCPGEFEDSALWILPSSIAS
                     TLTNAPATRRKFLHASREGRVREIPSPP"
     gene            complement(560..>2659)
                     /locus_tag="PKW60_13600"
     CDS             complement(560..>2659)
                     /locus_tag="PKW60_13600"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK00515.1"
                     /translation="LGTPGRRGIALIGPRGGGKSFLMEQLAGRIQAGRVPGVARDLAI
                     IKVDIAGIVSKYVGDTSRKLKAIFDYAKRVESCVLYIDEFQGLVTSGGPHATEHLSVL
                     KDLLTSSEYKSVQLVVATTSQEFDTYVRVDRALERRLSPFLIRSPQGEEEIQAVSMQA
                     KRLYKNAALLSDDLVKRAVGLAREYLPTQPTIQSAIELLAGAHSRFEFSQQALPLAQA
                     LLAECERQTGGKLRGIPSLGQTESLLGLLQEGLVGQPHITKALVEAYQLSTIGLGKQS
                     GPKCAFLFAGPPGTGKTHAARIYAEFLGCDFVHLHMGEYAVKDICTTKLIGVGPGWVG
                     SEVGGTLLNAVRQSPSAVVLFDEIDQAHPSVIDLLYQALDEGYMRDGFGNPVSFRNAT
                     LFFTTNRGDFSRGRRVGFAAHEGAETSVGLLTREEVVAALKRHFSEAFLSRITHALVF
                     GELSPAVLSWIAMNNIRAEALRLLETAALELEVEDIVGALIVAANGLETDARSVVKDI
                     LPNQFWRNVGKGIVRNWARMGQTGTLRICGLLPFAFPELEIVLLDDHPDETAKMIPER
                     AGHVETPRCSVSEAAACVRKEHDLVLLDLHWGKGPDDGLAILRQIRAEHPDVPVIIYT
                     ILSDHELKLKFFETGATAFVAKDDAAHLARCLRDIRTTKLIRKLSEAGYEHISYDFEV
                     LKTGGLTVTLTPVRRGP"
BASE COUNT          573 a          805 c          706 g          575 t
ORIGIN      
        1 ggagggcttg gaatttcgcg aacacggcct tccctgctcg cgtgaaggaa cttgcgccgt
       61 gtagccggcg cgttcgtgag ggtggacgca atggacgagg gcagaatcca cagggcagag
      121 tcttcaaact cgccggggca cggcgcatga ttgacgcatg ggaagaataa ccccattgtc
      181 ttcaatagcg cccgggccca gacgctttca ggaagcacca ggtaggcggc cttaccggaa
      241 ggatcaatgg tggcccagta tgcaagcagg gtttcacagg ttccctgtgc aataaattgc
      301 cggcaggcgt cccctctatc cgcaatgtac ttgtcaacgc tggcgcggaa ctggaaaggg
      361 tccatagcga gcggttcaag ttcttcaagc gcgcattgta cgcgcttcaa ctgaagctca
      421 tcgtccatgt gatcgcccag aaatatccgc aacgcgcaaa tggcttttgc atgccgcccg
      481 gcctcgtgat gcgggacagg cgcctcctcg taggttctga tcagccggtg aagccagtgt
      541 tctatggtct cgcgtgaact cacgggcctc tcctcaccgg agtcagggtc accgtcagcc
      601 ccccagtttt caagacctcg aaatcgtagg aaatgtgctc gtatccggct tctgacaact
      661 tccggatcag tttcgtcgtt cgtatatcgc gcagacacct ggccagatgc gcggcatcat
      721 cttttgccac gaacgcggtt gccccggttt caaagaactt gagtttcagc tcgtggtcgc
      781 tgaggatcgt gtagatgatt accgggacat ccggatgctc ggctctgatc tgacgcagta
      841 tcgcaaggcc atcatccggg ccctttcccc aatgcagatc cagcaacacg aggtcatgct
      901 ctttccgcac gcacgctgcg gcctccgata cagaacaccg cggtgtctcg acgtgcccag
      961 ctcgttcagg tatcattttt gccgtttcat cggggtggtc atccagaagc acgatctcaa
     1021 gttctggaaa tgcaaatggc agaaggccgc agattcgtag cgttcctgtc tgccccatcc
     1081 gcgcccaatt gcgcacaatg cccttgccca cattgcgcca gaactgatta ggcaggatat
     1141 ctttcaccac cgaccgggcg tctgtttcga ggccatttgc cgcgacaatt aaagccccaa
     1201 cgatgtcctc gacctccaat tccagagcgg ccgtttcaag gaggcgcaat gcctcggccc
     1261 ggatgttgtt catggcgatc cagctcagca ctgccgggct caattctcca aaaaccaggg
     1321 catgtgtaat tcgactcagg aaagcctcag aaaagtgccg tttcagcgct gcaaccactt
     1381 cttcccgtgt cagtaagccg accgaggttt ccgcgccctc atgcgcagca aacccaacgc
     1441 gcctgcctcg agaaaagtcg ccccggttgg tggtgaaaaa cagggttgcg ttcctgaacg
     1501 aaacgggatt gccaaatccg tcacgcatat aaccttcatc tagagcctga taaaggagat
     1561 cgatgacaga cggatgagcc tgatcgatct catcgaaaag aaccaccgcc gatggagact
     1621 gtctgacagc attaagaagt gtcccaccca cctccgagcc tacccagccc gggcccacac
     1681 cgatcagctt cgtggtgcaa atatccttta ccgcgtactc gcccatgtgt agatgcacaa
     1741 agtcgcagcc caggaattcc gcgtagattc tcgccgcgtg cgtctttccc gtgccgggtg
     1801 gaccggcgaa cagaaaggcg cactttggcc cgctctgttt ccccagccca atcgtcgaca
     1861 gttgataggc ttccacgaga gcctttgtaa tgtgaggctg ccccaccaga ccctcttgca
     1921 ataatccaag caaagactcg gtctgtccca gacttggaat tccccgcagt tttcccccgg
     1981 tctgtctttc gcattccgcc agcaacgcct gtgccagggg aagcgcctgt tgtgaaaact
     2041 cgaaacggct gtgtgctccc gcgagtaatt ctatcgcgga ttgaatggtg ggttgagtcg
     2101 gaagatactc tcgcgcaagg ccaaccgcac gcttgaccag atcatctgaa agcagggcgg
     2161 cattcttgta cagacgtttg gcctgcatac taacggcctg gatttcttcc tcgccttgag
     2221 gggagcgaat cagaaatggg ctgagacgtc tctccagggc ccggtccact cgaacatacg
     2281 tgtcaaattc ctggctggtc gtggcgacca caagctggac gcttttatac tcggacgaag
     2341 tcaagaggtc cttgagcacg ctcaggtgtt ctgtggcatg tggccctccc gacgtaacaa
     2401 gcccttgaaa ctcatcgatg tagagcacgc agctctccac tcgtttcgcg tagtcgaata
     2461 tggctttgag cttccttgaa gtatctccca catacttgct cacaatgccg gcgatatcta
     2521 ccttgataat ggccagatcc ctggcaacac cgggtacccg gcctgcctga atgcgccccg
     2581 ccagttgctc catcaaaaag gactttccgc ccccgcgagg gccaatcagc gcgatgccgc
     2641 gcctgccggg tgtgcccag
//
LOCUS       DAOPBQ010000518         2658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30340_length_2658_cov_5.068383, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000518 DAOPBQ010000000
VERSION     DAOPBQ010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30340_length_2658_cov_5.068383"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="PKW60_13605"
     CDS             complement(<1..117)
                     /locus_tag="PKW60_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK00516.1"
                     /translation="MRPVAPRPLRAARSGFTLIELLVVIAIIGILAAILLPAL"
     regulatory      complement(270..467)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            835..>2658
                     /gene="rpoB"
                     /locus_tag="PKW60_13610"
     CDS             835..>2658
                     /gene="rpoB"
                     /locus_tag="PKW60_13610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199146.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPK00517.1"
                     /translation="MAVAPDQSIDRPSLGRVPDAVELPDLIEIQTKSYSDFLQWNVPP
                     DERELKGLQEVFMDVFPIASPDGLTRLEFVHYAFAPPKYTVLECQERGLTYAASLKAL
                     LRLVKYEEVGTSNDLREQMMVEQEVFLGELPMMTPTGTFIVNGAERVIVSQLHKSPGV
                     SFETKVHQNGKILLTARIIPYRGAWLEFEYDVNDIIWLTIDRRSKILATTFLRAFGIE
                     ENDAILKLFYRTELVTLGRTRVKIGTKSHAPEDLVGRTLAEDVVDPETGEILVDAAEE
                     LTDAAVDRLMVSHVKQFHLIAEEDLAHDPSLAQTLSLDPSQTQEDAYREVYSRIRPGD
                     PATGATTRTFFQRMFFDASRYDFAPVGRYKINRKLGLEIPQETKTLTQEDVVATLQYL
                     VKLRSGEGVLDDIDHLGNRRVRAVGELLANQVRIGLVRMERTVRERMNFQDDEQLSPQ
                     NLVNPKPVSAVIKDFFGRSQLSHFMDQINPLAELTHKRRLSALGPGGLSRERAGFEVR
                     DVHATHYGRICPIETPEGPNIGLMASLSTYARINEYGFLETPYRKVESGRVTDQVEML
                     SAYDEDNYIIAQANAPLDKRGKFANPAVLSRHRGDFPQAEPG"
BASE COUNT          515 a          693 c          895 g          555 t
ORIGIN      
        1 cagcgcgggc aggagaattg cggcgagaat gccaatgatg gcaatgacga cgaggagttc
       61 gatcagggtg aatccggaac gggcggctcg cagggggcgg ggggcaacgg gtctcatagc
      121 gcgtgaggtc ctttcccggg gatgcagcgc atccgcggca atggcgacgc acacgagaca
      181 ggcggcctgc cgggaatggc atcggggaaa gacgggggca ggaagcgtaa cgggctttcc
      241 agcgcaactc tttccgcgaa gttgccgctt gtgatcgtgt gcccgtgggc tggttttctg
      301 gcttccggtt cgtcctactg gccgcgcctt cccatccgcc caaggcggac agtggccatg
      361 ccgcggcgtt cgtccccggt tacagcagcg cgtctgcgac ggattttcac cgtgcttccc
      421 tgtgcccacg cgccggattt gtctgtacgg gcggaagtat agaggaaacg gcgcgtttgg
      481 tcaagatttc ggccgaattt tggggcggga atagccctcg ttgcttgaaa tcgggttgtt
      541 cttgctgtct atcgagttga ctttggtggt ttttctggaa tagtgtaagc ggtgggcggt
      601 tcgtgcccgt ccaagccgag tttcctctgg accctggtga gctgcggagc gcacagtggt
      661 ctttttttga cgatatcgtt ttcttgacat gagttccgca atttggtatg tttcttgttt
      721 gtgttgctta ggcgaagtgt gtcttcgaga agaatcccat aacatcacgc tcccgtaaac
      781 cgtttgtttg caccggtgcc aagtggtttg caggacctga agaagggtat tcgcatggct
      841 gttgctcccg accaatcgat tgatcgcccc tcgttaggcc gtgttccgga tgcggtagag
      901 ctcccggacc tgattgagat tcagacgaaa tcctattcgg atttcctgca atggaacgta
      961 ccgcccgacg agcgggagct gaaagggttg caggaagtct tcatggatgt cttcccgatc
     1021 gcctcgccgg acgggctgac gcggctggag tttgtgcatt atgcatttgc gccgccaaag
     1081 tacacggtac tggaatgcca ggaacgcggc ctgacgtatg cggcgtcgct gaaggcactt
     1141 cttcgtctcg tcaaatatga ggaagtgggg acttcaaacg acttgcgcga acagatgatg
     1201 gtcgagcagg aagtgtttct cggagagctt ccgatgatga cgccgacggg cacgtttatc
     1261 gtgaacggcg cggagcgcgt cattgtcagc cagcttcaca agtcgccggg cgtgtcgttt
     1321 gagacgaagg tgcatcaaaa cggaaagatt ttgctgaccg cgcgcattat cccgtaccgg
     1381 ggcgcgtggc tggagttcga atacgatgtc aacgacatca tctggctgac cattgaccgg
     1441 cgctcaaaaa tcctggcgac gacgtttctc cgtgcgttcg gcatcgagga gaacgacgcg
     1501 attctgaagc tgttttaccg gaccgaactg gtcaccctgg gccggacgcg ggtgaagatc
     1561 gggaccaagt cgcatgcgcc ggaggatctg gtggggcgta cactcgctga ggatgtggtg
     1621 gatcccgaga ccggcgagat cctggtggat gcggcggagg aattgacgga cgcggcggtg
     1681 gatcggctga tggtgtcgca cgtgaagcag ttccacctga ttgcggagga ggatctggcg
     1741 cacgatccgt cgctggcgca gacgctgtcg ctggatccga gccagaccca ggaggatgcc
     1801 taccgcgagg tgtactcgcg catccggcca ggggatccgg cgacgggcgc gacgacgcgg
     1861 acctttttcc agcggatgtt ttttgatgcg agccgctacg attttgcgcc ggtcgggcga
     1921 tacaagatta accgcaaatt ggggctggag attccgcagg aaacgaaaac cctcacccag
     1981 gaagatgtgg tggcgacgct gcagtacctg gtcaagctgc ggagcggcga gggagttttg
     2041 gacgacatcg accacttggg caaccggcgc gtgcgcgcgg tgggtgagct gttggccaat
     2101 caggtgcgga tcggcctggt ccggatggaa cggacggttc gcgagcggat gaatttccag
     2161 gacgacgaac agctgagccc gcagaacctg gtaaatccga agccggtgag cgcggtgatc
     2221 aaggacttct tcgggcggag ccagttgtcg catttcatgg accagattaa ccccctggcg
     2281 gagttgacgc acaagcggcg tttgagcgcg ctggggccgg gcggtttgag ccgggagcgt
     2341 gccggtttcg aggtgcgcga cgtgcatgcc acgcactatg gccggatctg cccgatcgaa
     2401 acgccggaag gcccgaacat cggtctgatg gcgtcgttgt cgacgtatgc ccgcatcaat
     2461 gaatacggtt tcctcgaaac gccctaccga aaagtcgaga gcggccgcgt aacggatcag
     2521 gtcgagatgc tgtcggcgta cgacgaggac aactacatta tcgcacaggc gaatgcgccg
     2581 ctggacaaac ggggcaaatt cgcgaatccc gcagtgctgt cgcggcatcg tggagatttt
     2641 ccgcaggccg agccgggc
//
LOCUS       DAOPBQ010000519         2655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30381_length_2655_cov_5.063462, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000519 DAOPBQ010000000
VERSION     DAOPBQ010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30381_length_2655_cov_5.063462"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /gene="sucD"
                     /locus_tag="PKW60_13615"
     CDS             <1..748
                     /gene="sucD"
                     /locus_tag="PKW60_13615"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPK00518.1"
                     /translation="VTPGKGGQTVHGVPVFNSAAEAVAATGAQVSVMYVPARFALDAM
                     LDAAQSDLEMLVVITEGIPTMDALRGWRALTDAGKRMIGPNCPGLIAPGECKVGIMPG
                     AIHRPGSIGVISRSGTLTYEIVKELTDAGLGQSTCIGIGGDPIIGSRFIDLLPLFEED
                     PGTSAVVLVGEIGGQDEVDAAQYFQTHMSKPVVGFMAGVTAPPGRRMGHAGAIIGGED
                     DTAAAKIAKLEAAGIPVARVISEVPKLIPA"
     gene            complement(992..1588)
                     /locus_tag="PKW60_13620"
     CDS             complement(992..1588)
                     /locus_tag="PKW60_13620"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPK00519.1"
                     /translation="MGTRTGTAWERAIQKWYWKDREMDKGWINREDAFGETPLVRALR
                     CGNQTLINFMLGQDRDDEPAAPVDTPEDSLQSAAYWGAAQAVHALLENGADATGRDNS
                     GETPLHKAARNGRCEAVEALLEHGALVNVPDDQGLTPLHWATLAGDPEVAELLLEQGA
                     DPRASAGLLGDLSPRAIASLMGYDELVDVFNAHGAAVV"
     gene            complement(1590..2102)
                     /locus_tag="PKW60_13625"
     CDS             complement(1590..2102)
                     /locus_tag="PKW60_13625"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPK00520.1"
                     /translation="MSRSWIHQTDASGETPLNRALRCGFEGLAEFVLRELHQNKHKCA
                     PQPRPADACVDRSTAGFQPADGESWIGNSGNADTKLHKAIRDGYRDMVEAMLVFGADA
                     NETTASGMTPLHWCALTGDAELADVLVEHGARTDARAPGLGGLTPKAAAILMGYEEVA
                     DLLSACGGTG"
BASE COUNT          456 a          850 c          832 g          517 t
ORIGIN      
        1 cgttaccccc ggcaaaggcg gacagactgt gcacggcgtg cctgtgttca attcggcggc
       61 ggaagccgtg gcggccacag gcgcgcaggt gtccgtgatg tatgtgccgg cccgttttgc
      121 gctcgacgcc atgctcgatg ccgctcagtc ggacctggag atgctggtgg tgatcaccga
      181 aggcatcccc acaatggatg cgcttcgggg ctggcgcgcg ctgaccgatg ccggcaagcg
      241 gatgatcggg ccgaattgcc cgggcctcat cgctccgggc gaatgcaagg tcggcatcat
      301 gcccggggcc atccaccgtc ccggaagtat tggcgtcatc tcgcggagcg gcaccctcac
      361 ctacgagatt gtcaaggaat tgaccgacgc gggcctgggt cagtccacct gcatcggcat
      421 cggcggcgac cccattatcg gcagccggtt catcgatctg cttcccttgt tcgaggagga
      481 ccccggcacg agcgccgtcg tgctggtcgg cgagatcggc gggcaggacg aagtggacgc
      541 cgcgcagtat tttcagacgc acatgagcaa acccgtcgtc ggtttcatgg ccggcgttac
      601 cgcaccgcca ggccgccgca tggggcatgc gggtgcgatc atcggcggag aagacgatac
      661 cgccgcggcc aagatcgcaa agctcgaagc ggcgggcatt ccagtagccc gcgtcatcag
      721 cgaagtgccg aagcttatcc cggcctgatc gtacgggaaa cggaaggtgc cgggacggtc
      781 tcgagggcga gatgcggtgg tgagaccgtc ccggcgctgg gtttgaagcg tgtactacgg
      841 tgcgagccgc ccaccttcgc ggtccggcat cgctcggtgg ccgtccacgc ttcgccatct
      901 ggcaaaacct gcggtctggc tggagaggcg ggcaggcttt accgcgaggc ggggccagcc
      961 gcgccgggcg gcgcgggccc cgatccctga gctataccac ggcggcgccg tgcgcattga
     1021 atacatccac aagctcgtca tatcccatga ggctggcgat ggctcgcggg ctgaggtcac
     1081 ccagcagccc ggccgatgcg cgcgggtctg ctccttgctc aagcagcaat tccgcgactt
     1141 cggggtcgcc ggccagcgtg gcccaatgca acggcgttaa tccctggtca tccggcacgt
     1201 tgaccagcgc gccgtgttcc agcaaggcct ccacagcctc gcaacggccg ttccgcgccg
     1261 ccttgtgcag aggtgtttcg ccgctgttat cccggccggt ggcgtccgcg ccgttttcaa
     1321 gcagggcgtg aaccgcctga gccgctcccc aataggcagc actctggagg gagtcttccg
     1381 gcgtatccac cggcgccgcc ggttcatcat ctcgatcctg tcccagcatg aagttgatga
     1441 gggtctggtt tccgcagcgc aacgcgcgga ccagcggtgt ctcgccaaac gcgtcttccc
     1501 ggttgatcca gcccttgtcc atctcccggt ctttccagta ccacttctga atggcccgtt
     1561 cccatgcggt tcctgttcgc gttcccatat cagccggtac ctccgcaggc gctgaggaga
     1621 tccgccacct cctcgtaacc catcaaaatc gctgcggctt taggtgtcaa tcctcccaaa
     1681 ccgggcgccc gtgcatcggt cctggcgcca tgttccacca gcacgtccgc gagttccgcg
     1741 tctcccgtaa gcgcgcacca gtgcaacggg gtcatgccgg atgcggtggt ctcgttggca
     1801 tcggcgccga atacgagcat cgcctccacc atgtcccggt atccatcccg gatggccttg
     1861 tgtaattttg tgtcggcgtt ccccgagttt ccgatccagg actcgccgtc tgccggctgg
     1921 aaaccggccg tgctcctgtc aacgcaggca tcggcgggcc tgggctgagg ggcgcatttg
     1981 tgtttatttt gatgcagttc gcgcagcaca aactcggcca gtccctcaaa cccgcatctt
     2041 aaggcgcggt tcagcggagt ttcgcctgag gcgtccgtct gatgtatcca cgaccggctc
     2101 attccattca cctccacatt gccggcgggt ctcccgcccg ttcgtgcctg gtcaatatag
     2161 gagcacggtt catgccaagc gttgttgcag gttctggaaa gcgcgcgaga cgccgacttt
     2221 gcgaccaatg aaagagttac agaccgcgaa aaaaaacggt gaatgcgagt ggatggacag
     2281 gcaggacgtc gtcttgacga ttctcggcaa tagtgacaaa tatccgtcag tttctgaggc
     2341 gataagcgag acagaaaacg gggtccgggc tcgttttgcc ctgagcgcga ggacggctgt
     2401 ttacggcgct gcccaggtag gcacagatcg cctccaagcc ttggaaaagg ccggattttc
     2461 tctacttacg gcctcttttt atagtgttaa gcgggcaggc cgagcaggct caccacttca
     2521 ggccagcggg agcggtagat ctggctgccg aactggtcga ggtcgtcgac cagggcgatc
     2581 atgccgcctt cacgccattc tccgcctttt tcgagatact gagccacggt acgctgcagc
     2641 gaattctccc gcggg
//
LOCUS       DAOPBQ010000520         2651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30441_length_2651_cov_2.822034, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000520 DAOPBQ010000000
VERSION     DAOPBQ010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30441_length_2651_cov_2.822034"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PKW60_13630"
     CDS             complement(<1..718)
                     /locus_tag="PKW60_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00521.1"
                     /translation="MQRVAIIGMGPIGNRHADCYRAIPGCELVGVCDRITERADAASA
                     RLGVPAFYGAAEMLEQVQPSLCSVTTGGFEYGSDHYEPTLQALEAGCHVLGEKPISND
                     ITQAEEMVALASRKGLCYGINLNHRFTMLTRLAKQWVEAGRLGHLLFINMAMWIKNPA
                     ESSPWFQIKALHPHTIDVMRHFCGDIEAVQCFVTKAPGRSIWTTAHFNMKFSGGAVGG
                     LTGSYDIERGHPMERCEVAGT"
     gene            1074..2120
                     /locus_tag="PKW60_13635"
     CDS             1074..2120
                     /locus_tag="PKW60_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPK00522.1"
                     /translation="MGAFCTGHAAVAPNELPVVKSGNMLETLTLKELYAPVHSQIDAV
                     SARVGDFWTQALQLVHGSSMPAMQVGGKMLRPALCLLGAGVLGVSDTRRFVNLATSME
                     LLHLAALAHDDVIDRASIRRGMHTLNARWDDHAAILAGDYLVARSIELMTPYNSASII
                     STAVRSIREMAEAELKCFANGSEPMTEEDVLEIARKKTASLFAAACSAPALLLDDPHG
                     QTLYDYGIALGIAFQVVDDLLDLRQDAKVLGKPSCSDVVEGKQTLPILFMREELDSAG
                     RARLQRMRGAEMTPENREWVLDMLVATKAAERSEAVAREYAVQAHAAAAQLPAGPHRA
                     SLTGIVDFVIARTW"
     gene            2318..>2651
                     /locus_tag="PKW60_13640"
     CDS             2318..>2651
                     /locus_tag="PKW60_13640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK00523.1"
                     /translation="MSIALETTVLCDISQDEVQEPRVAVRAELDDSGAVARAKTGDVE
                     AFEYLVKRYRNEVFALAYHFLRSREQAWDVAQEVFIKAYNALTQFRGDASFKTWLMRI
                     TANQCKDYL"
BASE COUNT          515 a          823 c          775 g          538 t
ORIGIN      
        1 cggttcccgc gacttcgcag cgttccatcg ggtggccgcg ctcgatgtcg tagcttcctg
       61 tcaagccgcc caccgctccg ccggaaaatt tcatgttgaa gtgcgcggtc gtccagatgc
      121 tgcggccggg ggccttcgtg acaaaacact ggacggcctc gatgtcgccg cagaagtggc
      181 gcatcacatc tatggtgtgc gggtgcagcg ccttgatctg gaaccagggc gaactctccg
      241 cgggattctt gatccacatg gccatgttga tgaacagcag atggccgagt ctgcctgctt
      301 ccacccattg tttcgccagc cgggtgagca tcgtaaaccg gtggttcaga ttgatgccat
      361 agcacaggcc tttccggctg gccagagcga ccatctcctc cgcttgcgtg atatcgttcg
      421 agatgggttt ctctcccagc acgtgacacc cagcctccag cgcctgcagg gtcggctcgt
      481 agtggtcgct cccatattca aagccgcccg tggtcaccga gcacagactg ggctgcactt
      541 gctccaacat ctcggccgca ccgtaaaacg ccgggactcc cagccgcgcc gaggcggcat
      601 ccgcacgctc cgtgattcgg tcgcagactc ccacgagttc gcagccggga atcgcgcgat
      661 aacaatcggc atggcggttt ccgatgggcc ccattccgat gatggcaacg cgttgcacag
      721 gcaatccccc ttcatatcag cgagaaaccg gacaccgcca gtttcgctca gcacgacggt
      781 tggggcgcca ttttataccc cttctcttcg tgattccatc cggcgacagc gatccgcgcc
      841 ttggccaggg gcaagatccc ggttcaacgc ggcgcgcccg cttatattct ccgaagcggc
      901 tcgcacgttc tcctgcggcc ggaagaaaga gtcgtgggag taaatcacga ttgggacgcg
      961 tttggaaagg aggcatgcgc cgggtcgaaa gcactcagca ggcacccggg cacatgaatc
     1021 caaattgaac ccccggaatg ttcgcttgag acagcggaca tgaggagagc attatgggcg
     1081 cgttttgcac gggccacgcc gcagtcgcac cgaatgaact gcctgtggta aaatccggaa
     1141 atatgctcga aacgcttaca ctcaaagaac tttatgcccc cgtgcacagc cagattgatg
     1201 cggtttccgc ccgggtcggc gatttctgga cccaggccct gcagctggtg cacggctcat
     1261 ccatgcctgc catgcaggtt ggcggcaaga tgctgcgccc ggccttgtgc ctcctgggcg
     1321 ccggggtcct gggcgtctcc gacacccggc gatttgtgaa tctggccacc agcatggaac
     1381 tcctccatct ggccgcattg gcccacgatg atgtgatcga ccgcgccagt atccgccgcg
     1441 gcatgcacac gctcaatgct cgctgggacg accatgccgc catccttgcc ggcgactatc
     1501 tggtcgcccg ttcgattgag ctcatgaccc cctacaattc cgccagcatc atttccaccg
     1561 ccgttcgctc catccgggaa atggccgagg ccgaattgaa atgcttcgcc aacggcagcg
     1621 agcccatgac ggaggaggat gtgctggaaa tcgcgcggaa gaagaccgcg agcctgttcg
     1681 cagcagcctg cagcgcgccc gcgctgctcc tggacgaccc gcatggtcaa acgctgtacg
     1741 attacggtat agccctcggc atcgccttcc aggtggtaga cgacctcctg gatctccgcc
     1801 aggatgccaa ggtactcgga aaaccttcgt gttctgatgt cgtggaaggc aaacagacgc
     1861 tccccatcct gttcatgcga gaggagcttg actccgcggg ccgggcccgg ctgcaacgca
     1921 tgcgtggcgc ggaaatgacg ccggaaaacc gggagtgggt cctcgatatg ctggtcgcga
     1981 ccaaggcggc cgagcgttcc gaggctgtcg cgcgtgaata tgcggtgcaa gctcatgcgg
     2041 ccgccgccca gcttcctgcg ggcccccacc gcgcctctct tacgggcatc gtcgattttg
     2101 tgattgcccg gacttggtga cttcggcccg gtgtacccat ttcgctccgt ttccgcacct
     2161 gtgctccttc ccggtttttc cgcgggccct ttacgtcctt ccagcacagg ggctatcatg
     2221 aacggcggac atcttcccgg caaaagcata aggtgaattg tattaaatat ttcagcccag
     2281 aaatcgtcgg tattgtctgg gtgaggagaa tgggcaattg agtatcgcat tagaaaccac
     2341 ggttttgtgc gacatctcgc aggacgaggt gcaggagccg cgcgtagccg tgcgggccga
     2401 gctcgacgat tcgggtgcgg ttgcccgtgc gaaaacgggc gacgttgaag catttgaata
     2461 tctcgtaaag cgctaccgca acgaagtctt tgctcttgcc taccactttc tgcgaagccg
     2521 cgagcaggca tgggatgttg cccaggaagt atttatcaag gcatataacg ccctcacaca
     2581 gttccgcgga gacgccagct ttaagacgtg gctgatgcgg atcacagcga accagtgcaa
     2641 agattatttg a
//
LOCUS       DAOPBQ010000521         2645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30511_length_2645_cov_4.232432, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000521 DAOPBQ010000000
VERSION     DAOPBQ010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30511_length_2645_cov_4.232432"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1544)
                     /locus_tag="PKW60_13645"
     CDS             complement(3..1544)
                     /locus_tag="PKW60_13645"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine hydroxymethyltransferase"
                     /protein_id="HPK00524.1"
                     /translation="MGRPAVNNSTSNGAIAEYLRSTTPEQIDTGFLGYLSSLTEVARV
                     APQIARSIVQELADQRANLKLIASENYCSLATQLAMGNLLTDKYAEGYPGHRFYSGCD
                     NVDEVEAYACDQLKKLFGCDHAYVQPHSGADANLIAYWAILQTRVAVPALAELGVADP
                     SKLSREDWDRIREKTGNQRLLGMDYYSGGHLTHGYRHNISAMMFDSYSYGVSRETGLL
                     DYDELEKMVLEVKPLIFLAGYSAYPRKIDFKRLRAMADQVNAVFMVDMAHFSGLVAGG
                     VFEGDFNPVRHAHVVTTTTHKTLRGPRGGAVLCVEEFAESVNKGCPLVIGGPLPHAIA
                     SKAVAFTEANTPEFKTYARKIAENAQVLAQACLDEGLKISSGGTDNHLMLIDVTGFGL
                     TGRQAESVLRDCGITLNRNSLPYDVNGPWYTSGLRIGTPAVTTLGMGEAEMKEIASII
                     KLVLANTHPAAVDSGPNAGKPSKARYAIDDTVAAAARSRVARLLEAYPVYPQLDLALL
                     QKHFG"
     gene            complement(1831..>2645)
                     /locus_tag="PKW60_13650"
     CDS             complement(1831..>2645)
                     /locus_tag="PKW60_13650"
                     /inference="COORDINATES: protein motif:HMM:NF028228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK00525.1"
                     /translation="SSSFMAKKQKGSRVKEILSTSRSAESEIKSLWHSIQEKPWHYGV
                     GIAVTAAALLFTIGYQQHTAKAKRELYTRYARALEAGEPAEQAKQLEAAAASRSASPE
                     VIYMLGETALKAGEYDKAKAAFERLRQEFPGDKHAPDAVEAIGFIAQENEDYDGALKA
                     YTEIVEKWPQSFAARRQPLNIARVREAQGELAGAVQAYEDQTHQFPGSSVAQESEAAL
                     ARLRDSHPDLFPQEAPKTPPETPAAPASDATPAAAPDEPAAAPETAPEANPQ"
BASE COUNT          448 a          821 c          802 g          574 t
ORIGIN      
        1 gctcagccga agtgtttttg cagcaacgcc aaatccagtt gcggatacac cgggtaagcc
       61 tccagcagac gcgccacacg cgaccgggcc gcggcggcga cggtgtcgtc aatcgcgtat
      121 ctggccttgc tcggcttacc ggcattgggg ccggaatcca cagcggccgg gtgggtgttt
      181 gccagcacca gcttaatgat cgaggcgatt tccttcatct ccgcctcgcc cattcccagc
      241 gtggtcaccg ccggcgtgcc gattcgcagc ccgctggtat accaggggcc attcacgtca
      301 tagggcagcg agttgcggtt aagcgtaatt ccacaatccc gcaggacact ctcggcctgc
      361 cggcctgtca ggccaaaccc cgtcacgtcg atcagcatca ggtggttgtc cgtgccgcca
      421 ctggagatct tgaggccttc gtccaggcag gcctgggcaa gcacttgcgc gttctctgca
      481 atcttccgcg cgtaagtctt gaattccggt gtattcgcct cggtgaaagc caccgccttg
      541 gacgcgatgg cgtgcggaag cgggccgcct atcaccagcg ggcatccctt attcaccgat
      601 tccgcaaact cttccacgca gagcaccgcc ccgccccgtg gcccgcgaag tgttttatgg
      661 gtcgtcgttg ttaccacgtg ggcatgccgc actggattga aatcgccctc gaacacgccg
      721 ccggccacca atcccgagaa atgggccatg tccaccatga acaccgcgtt aacctggtcg
      781 gccatcgcgc gcaggcgttt gaaatcgatc ttgcgcgggt aggcgctgta tcccgccagg
      841 aagatcagcg gtttgacctc caggaccatc ttttcgagct catcgtagtc cagcaggccc
      901 gtctcccggc ttaccccata gctgtacgaa tcaaacatca tcgccgagat gttgtgccgg
      961 tatccatgcg tgaggtgacc gccggagtag taatccatcc ccagcaggcg ctggttgccg
     1021 gtcttttcgc ggatgcggtc ccagtcttcg cgcgacagct tcgagggatc ggcgacgccc
     1081 agctccgcca gggccggcac agccacgcgc gtctgcagaa tggcccagta ggcgatcaga
     1141 ttggcatccg cgccgctgtg cggctgtaca tacgcatgat cgcacccgaa cagcttcttg
     1201 agctggtcgc acgcatacgc ctccacctcg tccacgttgt cgcagccgga gtaaaaccgg
     1261 tgtcctggat atccctccgc gtacttgtcc gtcaggaggt tgcccatcgc cagctgcgtt
     1321 gcgagcgaac agtagttttc gctggcgatc agcttgagat tcgctcgctg gtccgccagt
     1381 tcctgcacga tcgagcgtgc tatctgtggc gctacgcggg caacctctgt caggctgctc
     1441 aggtatccca gaaagcctgt atcgatctgc tcgggcgtag ttgatcgtaa atattcggca
     1501 atcgcaccgt tcgaagtgga attattcacc gctggtctcc ccatcgcatt gcattggagg
     1561 cgttatggta gtcgttcccg aggcgccgtt gcaacaggct ttgcgaaacg ccccgtaacc
     1621 cgtcggaggt ctgcgggagg agcaaggcag cccggcaact gacgctctcc ggggacgcct
     1681 gaaatcaggc tcgccggagg atttagtggg caggattcag aagatcttac ccgcccggat
     1741 acgtaaacgg tcctgtattg cgcctttggc gtggaaaact gcgatccgcg ggtatccgcc
     1801 gcaaaacagc gtgctcgccc agacaaaaag ttattgcggg ttggcctcag gcgcggtttc
     1861 gggcgctgca gccggttcgt ccggagcggc tgccggagtc gcatcagagg ccggggcagc
     1921 cggggtctca ggcggtgttt tcggcgcctc ttgcgggaac aaatcgggat gggaatcacg
     1981 caggcgtgcc agggctgctt ccgattcctg cgcaacgctg gagcccggga actggtgggt
     2041 ctggtcttcg taggcctgca ctgcgcccgc gagctcgcct tgagcttccc ggacgcgcgc
     2101 gatattcagc ggctgccggc gcgccgcgaa gctctggggc catttttcga cgatttccgt
     2161 gtaggccttg agagcgccgt catagtcttc gttttcctgg gcgatgaacc cgatggcttc
     2221 gacggcgtcg ggcgcatgtt tgtcgcccgg aaattcctgg cggaggcgct cgaaagcggc
     2281 tttggctttg tcgtactcac ccgctttgag tgcggtttct cccagcatgt agatcacttc
     2341 gggcgaggcc gagcgactgg ccgctgcggc ttcaagctgt ttggcctgtt ccgccggctc
     2401 gccggcttcc agtgctctgg cgtagcgggt ataaagttcg cgcttcgcct tcgccgtatg
     2461 ctgttgatac ccgatggtga acagtaaggc ggctgcagtc acggcgatcc cgacgccata
     2521 gtgccagggc ttctcctgga tgctgtgcca gagcgatttg atttcgctct cggcgctccg
     2581 cgacgtgctg agaatttctt ttacccggct gcccttttgt ttctttgcca tgaatgagga
     2641 acttt
//
LOCUS       DAOPBQ010000522         2636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30653_length_2636_cov_4.004262, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000522 DAOPBQ010000000
VERSION     DAOPBQ010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30653_length_2636_cov_4.004262"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..282)
                     /locus_tag="PKW60_13655"
     CDS             complement(13..282)
                     /locus_tag="PKW60_13655"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HPK00526.1"
                     /translation="MDTHAMEHAQTRMLSDARADETVRVVRLLGGCGFKETLANLGVL
                     PGTALRVLTGGASGPQLVLCRGCRIAIGRGMAERVEVVQATSRQA"
     gene            complement(302..757)
                     /locus_tag="PKW60_13660"
     CDS             complement(302..757)
                     /locus_tag="PKW60_13660"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPK00527.1"
                     /translation="MPEERLSASLEDYLEAIFHVVQAKQASRAKDIADRLNVNSSSVT
                     SALHALKERGLVNYEPYDLVTLTPEGLRLAKEVVRGHEALRSFFVDVLGVENSEAEAA
                     ACGMEHAMSPLVLERLVRFIEFVGTASDARLQWDDKEGEFRSSLPNGRD"
     gene            1579..1920
                     /locus_tag="PKW60_13665"
                     /pseudo
     CDS             1579..1920
                     /locus_tag="PKW60_13665"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2503..>2636
                     /locus_tag="PKW60_13670"
     CDS             2503..>2636
                     /locus_tag="PKW60_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HPK00528.1"
                     /translation="MLDQALVHLNHGSFGAVPVAAVREQDRLRAQMEAAQVRWFTTLG
                     P"
BASE COUNT          507 a          852 c          771 g          506 t
ORIGIN      
        1 gctcgcacaa aatcaggcct gccgggaagt tgcctggacc acctcgacac gttcggccat
       61 gccccggcca atcgcgatgc ggcagccccg gcacaggaca agctgcgggc cgctggctcc
      121 gccggtaagg acccgcagcg ccgtgcccgg caatacgccg agattcgcca gggtctcctt
      181 aaaaccacac ccgccaagca atcggacaac gcgcactgtt tcgtcggcgc gtgcatcact
      241 cagcattcgg gtctgtgcat gttccatagc atgcgtgtcc atactcattg gctcgcggca
      301 gtcaatcccg cccattgggc agactgctgc ggaattcgcc ttccttgtcg tcccactgca
      361 atcgcgcatc tgacgccgtc cccacaaact cgatgaagcg aaccagccgc tccagcacca
      421 gcggcgacat ggcgtgttcc atcccgcacg cggccgcttc ggcttcgctg ttttcgacgc
      481 ccagcacgtc tacaaaaaag cttcgcaatg cctcgtggcc ccggaccacc tctttggcca
      541 atcgcaggcc ttccggggtc agggtcacca gatcatatgg ctcgtaattc accaggccgc
      601 gctctttcag cgcatgcagc gcgctcgtga ccgatgaact gttgacattc aaccggtcgg
      661 caatatcttt cgcccgcgat gcctgtttag cctggactac gtggaaaatg gcttccagat
      721 agtcctcgag gctggcgctg agccgttctt ccggcataat ctttcgtctt tcctgaaagt
      781 tttgatacgc caaacaatta tagtctcaac gaagcgccca gtcaattaat tccagctatt
      841 cttgaagtga caacaccgga tacccggaat gcgacagtct ttgctggaaa ccgccggaat
      901 cggcccgtcg ttggcaaaaa aaccggattc cgaggccaat tgaccgtgaa tcgagcccca
      961 attaagccta tggaaacgca gactttgaag atcctcgcag cataaggtcc aacccaggtt
     1021 cagctatcag cggcatatgc tggcagggca gactgcggaa aaatgaaaga cgatgagcgc
     1081 ggctggggtg catcttcttg acctctctgc gtcacttctg ttgaaatgcg tcgtaacgga
     1141 actagcacgg tagcgtgcgc ctcgaccggc catttcatgc cgttttgcgg aggcgcgctt
     1201 gcgattcagc ccgaacaacc agagggggtg cgtgtcatga gaggtattct gtcctgcctg
     1261 atggtcatgg tgcctgccac atttgcctgg caagcgagcg ctcaattggc cgaaccctgg
     1321 caggccagct atgccgggga cgacgccagc ggcccgcacg tcatcgcgtt gtggaatttc
     1381 gacgagagcg gagacacggt cgccgatgct tccggaaacc agcactcggg cacgttccag
     1441 ggtgcggtat ggcacccgga tggccgtttt ggcggctgtc tggagtccta tgctggatgg
     1501 cccgtcgaag atgcgcggca cgcggtccag gtcaaaaacg ctccgtcgct ctccccacag
     1561 ggcgcattca ccgtggagat gtggctgtgc cccgccgagg ccttcgaagg gtacgaagag
     1621 gcgttcctga tcgacaagaa atatgtcgct cataccgatt accagtggat actcgacaaa
     1681 cccaatacgg ccggccagcg gcgcatgcgt ctggttctcg gctttggaga ggattccgaa
     1741 acatggtggt ccccggaagc cgcggcattt ccaaaagaca cctgggcgca catcgcattt
     1801 tcttatgatg gtgccggtac ggttcacttc gtgcacaatg gccgttcgct gggcaagacc
     1861 accaagccgg gacgcggcag catctgcccg ggcaaccatt tcctctccct gggcgatcgc
     1921 gtcggcagcc tgtaccacgg tttccccgga cggatggacg agatccgcat cacgcagggc
     1981 gtgcgcgaat tccgtcccct gaccgttacg gcagaacatc tccggagcgc ctacctccgc
     2041 atggagcccg cgccgcagct gcagttccgg ctgaccaaca tgcgccggga caccctggcc
     2101 ggcgccacgg tgaccatcac cgtgccgggc ctgcctgcgc aaacttatcc cgtgccggaa
     2161 ctggatggag gcgcgtcgca tcttctcgag tacgcttttg acacccacct gcgccccgac
     2221 gtctaccccg tggtcataga gtcccgcctg ggcgacgggg cggacgcgtg gaccagccgc
     2281 gattcgtttg aggtgaccct cgtcgcccgc aagccgccgc tgcggatgcc ggtcgtcatg
     2341 tgggggatcg gcggcgtgga atcggtcacc gaaaacctcc ccgtgcttaa ggacatcgga
     2401 ttcactcatt gcctgggact gcgctgcgat ttcccggcaa tctgggaggc cggcgcccct
     2461 gctgccgccg tcaacgacaa ggaactcgag gccagccggc aaatgctgga ccaggccctc
     2521 gtgcacctca accatggctc gttcggggcg gttcccgtgg cggccgtccg ggaacaggac
     2581 cgcctgcgcg cccagatgga ggcggcgcag gtgcgctggt tcaccacgct cgggcc
//
LOCUS       DAOPBQ010000523         2632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30717_length_2632_cov_3.930539, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000523 DAOPBQ010000000
VERSION     DAOPBQ010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30717_length_2632_cov_3.930539"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..952)
                     /locus_tag="PKW60_13675"
     CDS             complement(323..952)
                     /locus_tag="PKW60_13675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00529.1"
                     /translation="MTDDHIIVCPSDADGEKLANDYWRKADGTVDPCDFWEVSYTYYG
                     WALRPEYVIDPTTDLNADPPTFDMGFLGAVGDVFANPTNSNSALRNFAAGTPWNSFEA
                     DITYTSAITGQSITCYRVREGIERFFITDINNPAGSAMAQSEIAVMWDSVWTPSESGE
                     SYFNHVPGGGNVLYMDGHVEFVRYPSEWPVCKAYVRMMDTLSAILNPHP"
     gene            complement(1243..>2632)
                     /locus_tag="PKW60_13680"
     CDS             complement(1243..>2632)
                     /locus_tag="PKW60_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00530.1"
                     /translation="TTILTAYAGQNEMLKSQSTPVDKVLDIAAGFDVDYSEHDLFGPP
                     LPEGGQFISRAIVSSLDAQALRLCLDLSALRDGEEVFVVDPIIPRAFGPYTAAQALEE
                     GRWLPVTEGDWTLLLVRSPFASPPALRLTSVAHFYRAFGECLKELWCNINVACESNPT
                     LQNVASGVGMLVIPRAGGDQGLCTCTLINNADTVEKEPYVLTSYHCIPKVVSAGQADV
                     VWDYRATSCGVSDPPPLGSLPRSNGYQMLATNSSLDITLMELDRVPNGAYGRYYAGWT
                     DRAVFAGEEITGIHHPDAKFMRISYGDILSSSVAGLGYTNQIRVLWHTGVTEPGSSGL
                     GLLLNSAGYQLIGTLSGGPSHICNASSNTDQFSSFRLFFPQVEGFLTGTTKPGGDCPA
                     EVAFKNQPHILEQLRKFRDESLIDSFVGKRLVETYYLLAPGMAKNIQRSPELAAAFRA
                     MALTFLRSTGKS"
BASE COUNT          546 a          723 c          821 g          542 t
ORIGIN      
        1 tgcttcgccg catcgtcgag aaggggaggg agggcaccgc ctgtctgttg agcattgctg
       61 gaacgccagc ccaatgtggc gccggatgcg gccgccaatt gaaggaatct ccgccgtttc
      121 atggttcgct gcactccgtt aggttggcaa gccctggtga agaccatgtc gctgtttggt
      181 tttggattat ccacatttgg gccgttgaat gcgagccgtg gaacgatagg cgcctgtacc
      241 cgctaaaaaa agacacacgc gcgcctggaa gcgcgcgtgt gttgaaccgc tttctgttgc
      301 cgctaaaccg gacggcgccg agttacggat ggggattgag aattgcgctg agcgtgtcca
      361 tcatgcggac atacgccttg cagaccggcc attcgcttgg ataccggacg aattcgacgt
      421 ggccatccat atagagcacg ttccctccgc cggggacatg gttgaagtag ctctcaccgc
      481 tctcggacgg tgtccagacc gagtcccaca tcacagcgat ttcactttga gccatggcgc
      541 tgcccgccgg attgttgata tcggtgatga agaaccgctc gatgccctca cggacccggt
      601 agcacgttat gctctggccc gtgatcgcgc tcgtataggt gatatcggcc tcaaacgagt
      661 tccagggggt tccggcagca aagttacgga gggcggaatt cgaattcgtg ggattggcga
      721 aaacatcgcc caccgctccc agaaagccca tgtcaaatgt cggcggatcc gcattaagat
      781 cggtcgtcgg atcaatcacg tactcggggc gcaacgccca gccatagtag gtatacgaca
      841 cttcccagaa atcgcaggga tcaacagtgc catccgcttt gcgccagtag tcattggcga
      901 gcttttcgcc gtcggcatcc gaggggcaga cgatgatgtg atcatcggtc atgtactcgg
      961 gatacaccga cgggccattg aagaactggt taaacgtgtt cggagtacca tggtacccat
     1021 cctcctgaaa gttgcctgga cacgcaggaa caccaagacg cgcccttatg atgacctttg
     1081 gtcgtggatt tgtcaatggc cgtcagttcg atagcattgt ggaaattggg cggtttttcg
     1141 agtaccgacg tttcgtcaac gcagtatatg cgggatggag ccatttcttc acagaccaca
     1201 agatgcgatt tgtgcgttta tctcgaaata taagcggaag cctcagcttt tgcctgtgct
     1261 tcgaagaaaa gtcagcgcca tcgcccgaaa ggcggcagca agctcaggcg acctttgaat
     1321 gttctttgcc atgcctgggg cgaggaggta atacgtctcg accaggcgct tgcccacgaa
     1381 agagtctatc agcgattcat ccctgaactt gcggagctgt tcgaggatgt gaggctggtt
     1441 cttgaatgcc acctccgctg gacagtcgcc gccgggtttg gtcgtgcccg tgaggaaacc
     1501 ctccacctgg ggaaaaaaga gccggaacga ggagaactgg tcggtattgc tggaagcgtt
     1561 acaaatgtgt gagggcccgc cgctgagggt gccgatgagc tgatagccgg cggaattgag
     1621 gagcaacccg aggcctgagg aaccgggttc ggtgacgccg gtgtgccaga ggacccggat
     1681 ctgattggtg tatcccagcc ccgcgacgct gctggagagg atgtcgccgt aggaaatgcg
     1741 cataaacttg gcgtcggggt ggtgaatgcc cgtaatttct tcacctgcga agaccgcgcg
     1801 atcggtccag cccgcgtagt agcggccata ggcgccgttg gggacacggt caagttccat
     1861 gagggtgata tcaaggctgc tgttagtcgc cagcatctgg tagccattgc ttctcggcag
     1921 gctgccaagc gggggcggat cgctcacgcc gcagcttgtg gcgcgatagt cccacaccac
     1981 gtccgcctgg ccggccgaga cgactttcgg aatgcaatga taggaagtca gcacgtaggg
     2041 ctctttctcg acggtgtctg cgttgttaat gagggtgcag gtgcacaatc cctggtcgcc
     2101 gccagcccgc gggatgacca gcataccgac tccggatgcg acgttctgga gggtgggatt
     2161 gctttcacag gcgacattga tgttgcacca gagctctttc aggcactcgc cgaaggcgcg
     2221 gtagaagtgc gctacgctgg tgagccggag cgccggcggc gacgcaaacg gcgagcgcac
     2281 cagcaggagg gtccagtcgc cttccgtaac aggcagccag cggccttctt ccagggcctg
     2341 tgcggcggta tacgggccaa acgcccgcgg gataatcgga tccaccacga acacttcctc
     2401 gccgtcgcgc agggccgaga ggtccaggca caggcgcagc gcctgggcgt cgagagagga
     2461 gacaatcgcc cggctgataa actggccacc ctcgggaaga ggcgggccga aaaggtcatg
     2521 ttcgctgtaa tcgacgtcga atcctgccgc aatatcgagg actttatcca cgggagtact
     2581 ctggctcttg agcatctcgt tctggcccgc atacgccgtg agaatggttg tt
//
LOCUS       DAOPBQ010000524         2625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30815_length_2625_cov_4.206226, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000524 DAOPBQ010000000
VERSION     DAOPBQ010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30815_length_2625_cov_4.206226"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1221)
                     /locus_tag="PKW60_13685"
     CDS             complement(142..1221)
                     /locus_tag="PKW60_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPK00531.1"
                     /translation="MNTTSKAASMLFLALTHVILVAGCATTPESNRLPEAAWRGLHVY
                     ISNRESLPVIIRAIENQLAPRGLNVLVVEVDFNFQYQSHPELASAAGMNKADVQQLLE
                     VCRRHGIELIPQFQCFGHQGSRPNIFLHTYPELMAPPNPDYSDPDHYHVSWNPLDPRV
                     NEIIFPLFDELIEAFEPRYFHVGMDEVMLFPDESTPHYNGETPAEVFAKAVNDYHDYL
                     VTQKDLTMLMWGDRLIDRSQIRYNWMESSSNGTAPAIDMIPKDIIICDWHYIRMVDYP
                     SIGFFQSKGFRVWPAAWRSPVAAAALMESAKHQAQGEQMLGFLCTTWCDPGEFARIML
                     EEADGGEHIVAQCAAETFRAVSGWW"
     gene            complement(1303..2370)
                     /locus_tag="PKW60_13690"
     CDS             complement(1303..2370)
                     /locus_tag="PKW60_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00532.1"
                     /translation="MRTPKTRLSVIAASILLIVALVLATALLIASRHERFAADGDSEA
                     ALQREGAAKDTALDTSGARSKGMALFSRAADLARLGDTSGALAAFEQAVGEWEERPWS
                     VPEEMRRRGRLFGKQLTLLSLENGDAAQALRSLSAGARASGRPVSALEELHARLDVAA
                     RDALWPESPFISLTSFIAGNTPGPILFGAAENTVFEVSIEPSGEMQSPCAVISVSSGE
                     GRPCFGLPAVVDLPSGTFGFRARVRADKPVPISMALRFWLPREKKELIYLPTAVHRVD
                     STWTWLAVEDDFLKYCYDGARAAGLDMSDAYIDRAGIAVPGEPNTFWIDRLELYLPGL
                     EGHPAKPENTEAVPMPGKPVE"
BASE COUNT          539 a          760 c          772 g          554 t
ORIGIN      
        1 cctgccgcct ttcatgaccg gcaggcagaa ccaagatttc aatgcgaccc agatgatctg
       61 gaattttttc tcgcgccacc ggctaaacgc gccgcagccc acggcaaacc gcctgcttcg
      121 ccgctgaaag cctgcgagag cctaccacca tcccgaaaca gcccggaagg tctcggcagc
      181 acattgggcg acgatgtgct cgccgccgtc cgcttcttca agcatgattc gggcgaactc
      241 tccgggatcg caccaggtcg tgcatagaaa tccgagcatc tgctcacctt gagcctggtg
      301 tttggcgctt tccatcagtg ctgccgctgc gacaggcgaa cgccaggcgg cgggccatac
      361 gcgaaaacct ttcgactgga agaacccgat cgacgggtag tccaccatgc ggatgtaatg
      421 ccaatcgcag atgatgatgt ctttcggaat catgtcgatg gcgggcgcgg tgccgttgct
      481 gctgctttcc atccagttgt accggatctg tgagcgatca atcagacggt cgccccacat
      541 gagcatggtg agatctttct gagtcacaag gtagtcgtga tagtcattga ctgcctttgc
      601 gaagacttcg gcgggggtct cgccgttgta atgtggtgtg ctttcgtccg gaaacagcat
      661 gacttcatcc atgcctacat ggaaataccg aggctcgaaa gcctcgatca gttcgtcaaa
      721 cagtggaaaa atgatctcgt tgaccctggg atcaagtgga ttccagctta catggtagtg
      781 gtccgggtca ctgtaatcgg ggttgggggg cgccatcagc tccggatagg tgtgcaggaa
      841 gatgttcggc cggcttccct ggtgcccgaa acactgaaat tgggggatca gctcaatccc
      901 atgccggcgg catacttcca gcaattgttg cacatccgcc ttgttcatgc ctgccgctga
      961 cgccagctcc ggatgggatt gatactggaa gttgaaatcc acctcgacca caagcacgtt
     1021 cagacctctt ggcgcgagct ggttttcaat ggcgcgaatg atgaccggca ggctctcgcg
     1081 attgctgatg tagacgtgca ggccgcgcca ggccgcttcc ggcaaacgat tagactccgg
     1141 cgttgtggcg catcccgcca ctagaatcac gtgtgtaaga gccaaaaaga gcattgaagc
     1201 ggctttggat gttgtgttca taacggactc catccttggc gggtcgattg tgggaagacc
     1261 cgcggcacag tgctcaacag cttccgcgca gtggcgtgat tgtcattcca ctggttttcc
     1321 gggcatcgga acggcttccg tattttcagg ctttgcggga tggccctcca agcccggcag
     1381 atacaattcg agccggtcta tccagaatgt gttcggctcg cctggcaccg cgatgccggc
     1441 ccggtcaata tacgcatcgg acatgtcgag gcctgcggca cgcgcaccgt cgtagcagta
     1501 cttcaaaaaa tcatcttcaa ctgcaagcca ggtccatgtg gagtccaccc ggtgaacggc
     1561 ggtcggcagg tatatgagct cctttttttc gcgggggagc cagaaacgca aggccatcga
     1621 aatgggtacc ggcttgtccg ctctgaccct tgcgcgaaag ccgaatgtac cggaaggtaa
     1681 atccaccacg gccggcaggc cgaaacaggg ccggccttcg ccggaagata cggaaatgac
     1741 ggcgcaaggg gactgcattt ctcccgaggg ttcgatggat acctcgaaga cagtgttttc
     1801 agccgctccg aacagtattg gccccggcgt attgccggca ataaacgatg tcagggaaat
     1861 gaaagggctt tccggccaca aggcgtcccg ggcggccacg tccaggcggg cgtgcagctc
     1921 ctccagcgca ctgacgggac gtccagatgc ccgcgcaccc gccgaaagag atcgcaaggc
     1981 ctgcgcagcg tcgccgttct caagcgacag gagcgtgagc tgtttgccaa acaagcggcc
     2041 cctccgccgc atttcttccg gcacggacca cgggcgttcc tcccactccc cgacggcctg
     2101 ttcaaaggcc gcgagcgcgc ccgatgtatc gcccagccgg gcgaggtcgg ccgcgcggga
     2161 gaacagtgcc attcctttgg aacgggcccc tgatgtatcc agggcggtgt cttttgccgc
     2221 gccttcgcgc tgcaacgccg cctcgctgtc gccatccgcg gcaaatctct catggcggga
     2281 tgcgatcaac aaggccgtcg ccagcacgag ggcaacaatc agcaaaatgc tcgctgctat
     2341 cacggacagt ctagtcttag gcgtacgcat cttcgggaag gttctcttgt tgtggtctgg
     2401 aaaatcgtag catatcgcag aattccgccg ggcggcaaat ttcgagcgct caattcttgg
     2461 tcttctttcc cgggcgaagg gattttggag gccctgcctg aaaggagagc attctcggtt
     2521 tgagcagggt gccggattca gtagggttgt aaacgcattg cctgagatgg ctgtgtttat
     2581 ctcgagccag tgatttgttc tgtgctgaaa tgagtccaca caagc
//
LOCUS       DAOPBQ010000525         2623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30843_length_2623_cov_4.727414, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000525 DAOPBQ010000000
VERSION     DAOPBQ010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30843_length_2623_cov_4.727414"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(642..1190)
                     /gene="nuoE"
                     /locus_tag="PKW60_13695"
     CDS             complement(642..1190)
                     /gene="nuoE"
                     /locus_tag="PKW60_13695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPK00533.1"
                     /translation="MPQIQIQEPVKTNGVRERHGAAIAELERKYPTRRSLLLPVLWLI
                     QEEDGWISAETMEEAAEICECTTAEVMEVVSFYQMYNRAPVGKYVLGICGTLPCALCA
                     ADGLYEYLKEKLGIGWNETTADGLFTIQRRECLGACSEAPVMLVNQETETRLTRAKVD
                     GILEECRQGKRKPYFTGRNNKQ"
     gene            complement(1233..2390)
                     /gene="nuoD"
                     /locus_tag="PKW60_13700"
     CDS             complement(1233..2390)
                     /gene="nuoD"
                     /locus_tag="PKW60_13700"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HPK00534.1"
                     /translation="MTLNMGPQHPSTHGVLRLVLQLEGETVIDLKPIIGYLHSGKEKL
                     AENKTYHMFIPYTDRLDYLSPMANNTAFVLSVERLLGIEVTERCKYLRTILCELARIS
                     SHLLCIGTHVMELGAMTVFLYTFREREALYDLFEEICGARFTVSYMRVGGVARDYPDG
                     WLDRVRKFIDEFPKKWQDYYDLTVNNDIYIMRTRGVGTISREEAISYCLTGPSLRASG
                     VDYDIRKKEPYLVYDRVDFNVPVRHEGDCYARFLNRLDEMLESVKIIRQCLDQMPKDG
                     PINIDNPKVIYPPRSRVKASMEDLIHHFLLASEGIYVPEGEAYHPIEASKGELGFYIH
                     SRGGYEPNRLKIRSPSFVNLQSLGHMSKGALMADVVAIIGSIDLVLGEIDR"
BASE COUNT          480 a          816 c          701 g          626 t
ORIGIN      
        1 cacgtcgtcc ggggtctgct cctgaagcgc gcgcttgtac ccgtcatacc cccccctggc
       61 ctggtaatcg gtcagcgact ccacggaacc ggaataaacc gttccggtga cgccggcggt
      121 caggatcggg ctgtagttct ggattgctgc tgtcgccatg gattgagtcc aaactcgaat
      181 ttcgaatagc gaaaatcgaa acaaattcga attttcaagc tatgaaacag tctcccacta
      241 ttgggagcca cactttctaa gaatcgatcc ggagattttc attaattcgt cagcctcatt
      301 cgccagtgat tcgcattccc gttgaacttc ctttgggttt tgtgattcga ccagtcgaag
      361 ccaatatcgc gtctctttgg cttcaatatg gtttgcaccg attgagcccg acgagcgaat
      421 cacctgcgtg cgtcctcgcg gttgcagatg gttttctcaa gccgtttagc aaggtttctg
      481 acgttgcgcg cgaatagaaa agtcctttct tcaagacagt aactcttgcg tataggtctg
      541 tcgaaagcgt cgatgtcttt cgaggattcc aatctcttcc ctctgttatt ccatcatttg
      601 agattgaaat ttgtttcgaa tttcgaattc atatttcgaa tttattgctt gttgttcctt
      661 cccgtaaaat acggttttct cttcccctgc cggcattctt ccaggattcc gtccactttc
      721 gcccgcgtca gccgggtttc ggtctcctga ttgaccagca tcaccggcgc ctcgctgcac
      781 gcgcccaggc attcgcgacg ctggatcgtg aacaatccat ccgcggtcgt ctcgttccag
      841 ccgattccca gcttttcctt caggtattca taaagcccgt cggcggcgca cagcgcacag
      901 gggagcgtgc cgcagatccc cagcacatac ttcccgaccg gggcccggtt gtacatctgg
      961 tagaaactga cgacctccat cacctccgcc gtcgtgcact cgcagatctc ggccgcctct
     1021 tccatcgtct ccgccgagat ccagccgtcc tcctcctgga tcagccacag cacgggcagc
     1081 agcagcgaac ggcgcgtcgg gtacttccgc tccagctcgg caatcgccgc gccgtgccgt
     1141 tcgcgcacgc cattggtttt gacgggctcc tgaatctgta tctgaggcat cagccgaccc
     1201 ttccaacctc gcttgcttga ttccgcatgg cgttaccgat cgatctcccc cagcacgagg
     1261 tcgatcgaac cgataatcgc caccacgtcc gccatcagcg cccccttgct catatgaccg
     1321 agcgactgca gattcacgaa cgaggggctc ctgattttca gccggttggg ctcgtaccct
     1381 ccccggctgt ggatgtagaa ccccagttcg cccttgctgg cctcgatcgg gtgatacgct
     1441 tcgccctcgg ggacgtagat gccctcgctg gccagcagga aatggtggat caggtcctcc
     1501 atcgacgcct tgacgcgcga gcgcggcggg tagatcacct tcggattgtc gatgttgatc
     1561 ggcccatcct tgggcatctg gtccaggcac tgccggatga tcttgacgct ctccagcatc
     1621 tcatccagcc ggttgagaaa tcgcgcgtag cagtcccctt cgtggcggac cggaacgttg
     1681 aaatcgaccc ggtcgtagac gagatacggc tctttcttgc ggatgtcgta atcgaccccc
     1741 gatgcccgca ggctcggccc cgtcaggcag tagctgatcg cctcctcgcg gctgatcgtg
     1801 ccgaccccgc gcgtgcgcat gatatagatg tcgttgttga cggtcaggtc gtagtagtcc
     1861 tgccactttt tcgggaattc atcgataaac ttgcggacgc ggtcgagcca gccgtcgggg
     1921 tagtcgcgcg cgacgccgcc gacccgcatg tagctgacgg tgaagcgcgc gccgcagatt
     1981 tcctcaaaca ggtcgtagag cgcctcgcgc tcgcggaacg tgtaaaggaa gacggtcatc
     2041 gcgcccagtt ccatcacgtg cgtcccgatg cagagcaggt ggctggagat gcgcgccagt
     2101 tcgcaaagaa tcgtccgcag gtacttgcag cgttccgtca cctcaatccc gagcagccgc
     2161 tcgacgctca gcacgaacgc cgtgttgttg gccatcggcg agaggtaatc gagccggtcc
     2221 gtatacggaa tgaacatgtg gtaggtttta ttctcggcca gcttctcctt gcccgaatgg
     2281 agatagccga tgatcggctt gaggtcgatc accgtctcgc cttccagttg caggaccagg
     2341 cgcagcaccc catgggtgga cgggtgctgc gggcccatgt tgagcgtcat gatttcgccg
     2401 gtcgggaaat cctgctcaac agtccgccgg agggcgtgcg ggttgcgctc aacgatcggt
     2461 gtgttggcca tcatccttca ctcagtgaga attcgcccct cactctaaat ttgtccctcg
     2521 tcctcgtgat cggccccaaa ggtctgccca gattcggatt ctcggctttc ttctctcgcc
     2581 ctcggctctt gaatcaactt gcatttcctc gataaccgcg agg
//
LOCUS       DAOPBQ010000526         2615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30958_length_2615_cov_4.050000, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000526 DAOPBQ010000000
VERSION     DAOPBQ010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30958_length_2615_cov_4.050000"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKW60_13705"
     CDS             complement(<1..271)
                     /locus_tag="PKW60_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPK00535.1"
                     /translation="MTTAGQLRRLLDQFLEASVFESGLADKSLDAYAGDLEAYTAYLE
                     QNGVTEPGQATRDHVLGHLIALRKSGLSARSAARHLSAIRRFHRFL"
     gene            complement(327..911)
                     /locus_tag="PKW60_13710"
     CDS             complement(327..911)
                     /locus_tag="PKW60_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPK00536.1"
                     /translation="MMNNDEASEKQPVEALAARLLKTRTILISGSVDEELAERVITEL
                     LILDGESHDPIRVFITSPGGHVDAGLAIHDVMRFVKSELIAIGAGWVASIAVPILFGA
                     PKKNRLALPHTRFLLHQPSGGAAGQMKDIRIEAQEIMKIRKRLNEMIAQETGQSYEKV
                     YEDSDRNFWMSAEEALEYGVIARVIESIDDIAKK"
     gene            complement(1007..1801)
                     /locus_tag="PKW60_13715"
     CDS             complement(1007..1801)
                     /locus_tag="PKW60_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPK00537.1"
                     /translation="MPYVHGYSDYEAGRLRNSARALGDLLHAGVRYPADARVLEAPCG
                     VGAQTGFLAANNPGTRFVCMDISAASIRMASEENPGLPFLAGDLYALPFSNATFEHAF
                     ACYVLEHLERPGLALENLLRVLKPGGTLTSIEGDHGSCFFYPETPEALAAWACLPRLQ
                     AMLGGDSLIGRRLYSVLARAGFEDVRVEPIRIYCDPSLPHMMNGFVEDTIVGMLQGIE
                     PEVVERGLLDAATFRKGVADILAIKTHPDGAFCYTFFRAVGRKPEE"
     gene            complement(1898..>2615)
                     /locus_tag="PKW60_13720"
     CDS             complement(1898..>2615)
                     /locus_tag="PKW60_13720"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00538.1"
                     /translation="GQIPNIVKNPKQMLGKVDAVLVDHRHPKYHLPAVTPFAERGIPV
                     FVDKPFCFEPKEGVAFLKLAKKAGAPITSFSVVPMQASFQKFKKGMESIGTVVAGSTY
                     GPCDLESQWGGVFFYGIHQVEMTLMAFGYDVKSALITKNGNGSTGQLLYRDGKIVTMN
                     LVKEGAPGFGISATGSEKSIHSSLPMDKSPYLAGVKTFCGMFKTRVEPISHDFLLRPV
                     QVLDALARSKKSGRMEKVTA"
BASE COUNT          504 a          846 c          725 g          540 t
ORIGIN      
        1 gcaggaagcg gtgaaaccgg cggatcgcgc tcaggtgcct cgccgccgac cgcgcgctca
       61 ggccgctttt ccgcagcgca atcaggtgcc ccagcacgtg gtcacgcgtg gcctggccgg
      121 gctccgtgac gccgttctgt tcgagatacg ccgtatatgc ttccagatcg cccgcgtacg
      181 cgtcgagcga cttgtcggcg agccccgatt caaaaacaga cgcctccagg aattgatcca
      241 gaaggcgtct gagctgtcca gctgttgtca tgtcattccc gtttccgccc ggaagggccc
      301 attgcgatgc gggaattcac gcgccactat tttttcgcga tatcgtcgat ggattcgatc
      361 acgcgcgcga tcacgccgta ttcgagggcc tcctcggcgc tcatccagaa gttgcggtcg
      421 ctgtcttcat agactttttc gtacgactgg ccggtttcct gcgcgatcat ctcgttcagg
      481 cgcttgcgga tcttcatgat ctcctgcgct tcaatacgga tatccttcat ctggccggcc
      541 gcccctccgc tgggctgatg cagcagaaac cgggtatgcg ggagggcgag ccggttcttt
      601 ttcggcgcgc caaacagtat cggcaccgct atgctcgcga cccatcccgc gccaatcgcg
      661 atgagctccg atttcacgaa gcgcatcaca tcatgaatgg ccaggcctgc gtccacatgc
      721 ccaccgggcg acgtgataaa cacccggatc gggtcatgac tctccccatc caaaatcaga
      781 agctccgtaa tgacccgctc ggccagttct tcatccactg accccgatat caggatggtg
      841 cgggtcttca aaagccgtgc cgccaacgcc tctacgggct gtttctcgct cgcttcgtca
      901 ttattcatca tggattacgc ctcgctcctg ttcgttgatg cgaaggcaca ttgtgccatc
      961 cgcccgcaaa acagttcaaa taccgggcgt ttcgggcgcc gcacagtcat tcttcgggtt
     1021 tacggccaac agcccggaaa aacgtataac aaaacgcccc atcggggtgg gttttaattg
     1081 ccaggatgtc cgcgactcct tttcgaaagg tcgcggcatc caggagaccc cgttcgacca
     1141 cctccggttc aatcccctgc aacatgccga caatggtatc ttccacgaac ccgttcatca
     1201 tgtgagggag gctgggatcg caataaatcc gaatgggctc gacccgcacg tcttcaaagc
     1261 ccgcgcgcgc cagcacggag tacaggcggc gcccgatcag gctgtccccg ccgagcatgg
     1321 cctggagcct cggcaggcat gcccacgccg caagggcctc gggcgtctcg gggtagaaga
     1381 aacacgaccc gtgatcaccc tcaatggagg tgagcgttcc accgggtttc agcacgcgca
     1441 gcagattctc caatgccagg ccgggacgct ccaggtgctc gagcacgtaa cacgcgaagg
     1501 catgctcaaa cgtcgcgttc gagaatggca gcgcataaag gtctcccgcc agaaacggca
     1561 ggccgggatt ttcttccgat gccatccgta tcgaggccgc ggagatgtcc atgcacacaa
     1621 aacgcgtccc cgggttgttc gctgcgagaa acccggtctg cgcgccaacg ccgcacggag
     1681 cttccagaac acgcgcatcc gcgggatagc ggacaccggc gtggagcaga tcgccgaggg
     1741 ctcgcgccga attccgaagc cgtcctgcct cgtagtctga gtatccgtgc acgtatggca
     1801 tcgccgttcg cccaatcccg gcaggggagc catccccggg ggctatgcct ttcgggtggt
     1861 cctccgcgat cctcccccag cgcttttctc cggcaggtta tgcggttact ttttccatcc
     1921 ggcccgactt tttcgaccgg gccagcgcat cgagcacctg gaccgggcgc agcaggaaat
     1981 cgtgggagat cggctcgact ctcgtcttga acatcccgca gaaggtcttt acaccggcca
     2041 ggtacgggct tttatccatg ggcaggctgg aatgaatcga tttttcgctc ccagtggctg
     2101 aaattccaaa cccgggcgca ccttccttca cgagattcat cgtgacgatt ttgccgtcac
     2161 ggtacagaag ctgcccggta gatccgttgc cgttcttcgt gatcagcgcc gatttgacgt
     2221 cgtatccgaa ggccatcagg gtcatctcga cctggtgaat cccgtaaaag aacacaccgc
     2281 cccactggct ctcaaggtcg cagggtccgt acgtcgagcc cgccacgacc gtgccgatgc
     2341 tttccatacc cttcttgaac ttctgaaagc tcgcctgcat cggcaccaca ctgaaggagg
     2401 taatcggtgc gcccgctttc tttgcgagct tcagaaacgc cacgccttcc ttcggctcaa
     2461 aacaaaacgg cttgtcgaca aagaccggga ttccgcgctc agcgaacggg gtgaccgcag
     2521 gcaaatggta tttcggatgc cggtgatcca ccagcaccgc gtccactttg cccagcattt
     2581 gtttcgggtt cttgacgatg ttcgggatct ggccg
//
LOCUS       DAOPBQ010000527         2614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_30981_length_2614_cov_2.201250, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000527 DAOPBQ010000000
VERSION     DAOPBQ010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30981_length_2614_cov_2.201250"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..2019
                     /locus_tag="PKW60_13725"
     CDS             97..2019
                     /locus_tag="PKW60_13725"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPK00539.1"
                     /translation="MTTSAALTHIQVGAVLLVLLTVVTLRPAAAHPALVRDLSPITLL
                     PVARHPAITLVSKGRARAVVYVADPHPSANLTVLLKEFIEDVKLSTGAELTVVTTPPP
                     ANQPAIVIGDSAESRAAGIDAAHLPIEGFVVKTARNRVFLVGSMQPLPEVHAQSLGGN
                     PYANDGTAWAVADFIERFVGIRWYWPTTAGGRSVVKRTTLAIPPTYYTDQPVFRKREF
                     YPRVGYRKGDWRAVWWDEQALTLPETLLPAGTEHIAMQPLLAGLRSGNSWPYIIKVHE
                     PQHFMREWEKWSKHPEMFQQKADGTRDLNMLCYSSQETLDYLLHGCEDAWDHGKPVSW
                     VTATCVTVSPGDYPVRCYCEKCAPLYAPDRAPYGASSKVMGLFVKRVCEAVKARWPEK
                     NVLYLPYWNYTECPDGIDFPDNLNVQMCTMAFGLMRQAEPRAKMEQRLRTWSMKIGGP
                     ITTWEYSHRVPEWTYAPVQYPHLVQDYYRTNRELLAGSFLNGGQIAEWSTAAPTAYCW
                     MKVLWNPDVNIEAMLDEMCRRMYGKAAGTCRALLRLECDRWEKAPWREGLGDAGHVSP
                     AVIADSYPPDVVATMIALRDRARREMAGDTLAEQRFAYWTWTFDHFLIEAQDAWKQTG
                     IVHEGTAGAKDIPHRY"
     gene            2181..>2614
                     /locus_tag="PKW60_13730"
     CDS             2181..>2614
                     /locus_tag="PKW60_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00540.1"
                     /translation="MMREYLAANELLSTVTQPGLEVFQITTDPDAGSNLFYNYHQAFE
                     PTSRFMLFNRTGKDGAGLWLCDLEDDFTLEPVTGDDERLAGGHQGMAFSPDGHWIWYS
                     LFADDRLELRRRPLTGGPMETVFDLDAIRHEFGGRKIHGGRWL"
BASE COUNT          525 a          740 c          818 g          531 t
ORIGIN      
        1 cccctccgag atccgcatcc acccgaacga ctggggaacc tggatcttac agtgagcgga
       61 gtgagcacga tgaaccttct ccatggaaaa cagcgtatga caacaagcgc tgccctgact
      121 cacatacagg taggcgctgt gctcctggtg ttactcacgg tggtcacgct acgacccgcc
      181 gcggcgcatc cggctctcgt tcgcgatttg tcacccatta cgctgctgcc ggtggccaga
      241 catcccgcca tcacacttgt gagtaaaggg cgggcgcgtg ctgtggtcta tgtggccgat
      301 ccacacccga gtgctaacct tacggtgctg ctcaaagaat tcatcgagga cgtgaaattg
      361 agtaccggtg ccgagttgac agtggtcacc acgccaccac cggccaacca gccggcgatt
      421 gtcattggcg atagcgcgga atcgcgggcc gcgggaatcg acgccgctca cctgcccatc
      481 gagggctttg tcgtgaaaac cgcgcgcaat cgtgtttttc tcgtggggag tatgcagcca
      541 ttacccgagg tgcacgcaca gagtttgggg gggaatccgt atgcgaatga cgggacggca
      601 tgggccgtcg cggattttat tgagcgcttc gttggcatcc gctggtattg gcccacgacg
      661 gcaggggggc gcagtgtggt gaagcgcacc accttggcga ttcctccgac gtattacacc
      721 gatcagcccg tcttccgcaa gcgtgaattt tatccgagag tcggctaccg caagggcgac
      781 tggcgggcgg tctggtggga tgaacaggcg ctgacgttgc ctgaaacact gctgccggcg
      841 ggcacggagc acatcgccat gcagccattg cttgccggtc tccggtccgg caatagctgg
      901 ccctacatca tcaaggtaca tgagccgcag catttcatgc gggagtggga gaaatggtcg
      961 aagcatccgg agatgtttca gcagaaagcg gacggcacgc gggacctgaa catgctttgc
     1021 tacagttccc aggagacgct cgactatcta ttgcacggct gtgaggatgc ctgggatcac
     1081 ggaaagcccg tctcctgggt cacggcgacc tgtgtgaccg tctcgccagg ggattatcct
     1141 gttcggtgtt actgtgaaaa gtgcgcaccc ttgtatgcgc ctgaccgcgc tccctatggg
     1201 gcgtcatcga aagtcatggg cctgtttgtg aagcgagtgt gcgaagcagt caaggcacgc
     1261 tggcctgaga agaacgtcct gtatttgccg tattggaatt acaccgagtg cccggacggc
     1321 atcgatttcc cggataacct gaacgtgcaa atgtgcacca tggcgttcgg ccttatgcgc
     1381 caggcggagc cgcgtgcgaa gatggaacag cgcctgcgca cctggagtat gaaaatcggc
     1441 ggtcccatca ccacctggga atattcgcat cgggtgccgg aatggaccta tgccccggtg
     1501 caatatcccc acctcgtgca ggactattac cgcaccaatc gtgagctgct cgcgggatcg
     1561 tttctgaatg gtggccagat tgccgaatgg tctacggcgg cgccgaccgc ctactgctgg
     1621 atgaaagtgc tatggaatcc cgatgtaaac atcgaggcca tgctggacga aatgtgccgc
     1681 cggatgtacg gcaaggccgc gggcacgtgt cgtgcgttgt tgcgcctcga atgcgaccgc
     1741 tgggagaaag cgccatggcg cgaagggctg ggggatgccg gccatgtttc ccccgcggtc
     1801 atcgctgatt cctatccgcc cgacgttgtg gccacgatga tcgcgctgcg cgaccgcgca
     1861 cggcgggaga tggccggcga tacattggct gagcagcgtt tcgcctactg gacgtggaca
     1921 ttcgaccact tcctgattga ggcacaggat gcctggaagc aaaccggtat tgtgcatgag
     1981 ggcaccgcgg gagccaagga cataccgcat cgttactgat cgtccggatc actgtgtttt
     2041 gtctggagtg tatgcgtcag tatgcgtaag gatgcgctga aaatcatccc aacgttcacc
     2101 gccgagccac gcatgttgca tgcgtatcgc cggagagcgc gtagatcaac ggcgccgtgg
     2161 ccttctgatg gaaagggacc gtgatgcgcg aatatcttgc agcgaacgag ttgctgagca
     2221 cggtaacgca gccgggtctg gaagtgtttc aaatcaccac cgacccggat gccggcagca
     2281 acctgttcta taattatcac caggcgtttg agccaacctc gcgatttatg ctgttcaatc
     2341 gcaccggcaa ggatggcgca ggcttgtggc tgtgcgactt ggaagatgac ttcaccctgg
     2401 agccggtcac tggcgatgac gaacgcctcg cgggcggcca tcaagggatg gcattctcgc
     2461 cggacgggca ctggatctgg tacagtctgt tcgccgatga ccgactggaa ctgcgccggc
     2521 gtcccctgac cggtgggccc atggaaaccg tcttcgatct tgatgcgatt cgacacgagt
     2581 tcgggggacg gaaaattcac ggcggccgtt ggct
//
LOCUS       DAOPBQ010000528         2593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_31329_length_2593_cov_4.236013, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000528 DAOPBQ010000000
VERSION     DAOPBQ010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31329_length_2593_cov_4.236013"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PKW60_13735"
     CDS             complement(<1..1150)
                     /locus_tag="PKW60_13735"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPK00541.1"
                     /translation="MNAIRTVSITLIFALTLGGSAMAQWKPVEGQLMTRWAEDVSPDN
                     VWPEYPRPQMTRDRWHNLNGLWDYAITPSTATSVPKAWEGQILVPFAIESALSGVKKA
                     VQPDQTLWYRRSFTLPENLEGQRLLLHFNAVDWECAAYVNGTFCGRHQGGYDPFYFDI
                     TSALIPGEQQELVVSVWDPTDTGYQPRGKQILDPHGIWYTAVTGIWQSVWLEPVPRAH
                     ITHLAMTPDIDRGVLRLTATAEGTAPGDEIVAVALDGEAPVVGSAGQPGTAFDIPIEN
                     ARLWSPDSPFLYNLTVTLTRGGQVLDEVKSYFGMRKIAVAKDDRGINRLFLNNEVLFQ
                     LGPLDQGWWPDGLYTAPTDEALRYDVETTRAMGFNMARKHVKIEPLRWY"
     gene            1348..>2593
                     /locus_tag="PKW60_13740"
     CDS             1348..>2593
                     /locus_tag="PKW60_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00542.1"
                     /translation="MRGRRAFMRMAFTLAAALVLACSVHAAPHDGFLLRAGQKLFPLG
                     FYELPESDEGLQAMADAGVNLVRCQNRHDLDRLHALGMQGVMPLPLEQGPTEDFRARV
                     ADVADHPALAVWEGPDEVVWNYTAFSGLFRTKGIYKTRDAWWTQAPEARAYAAEQAAT
                     IVPNMRAAVALIRELDAQQRPVWINEAKNSDVYYVRQYLDFVDITGCDYYPVHEDERR
                     IEEIVGATQRWNQIGRGMPVYMVLQAFSWHELGEYYGAQREAYPTFAESRFMAYAAIV
                     HGADGILYWGSSFLKSEACRASIYALTKELAALQPFLTAPDVMGVRVSAIEDYHPDVA
                     PRIGWMVRRSGDDWLVALVNEDDIRHTAVIVEGLEALNGRTLGQLYTDDAAVVARGEL
                     IARMQPKAVHVYATSRDYEADCG"
BASE COUNT          475 a          680 c          934 g          504 t
ORIGIN      
        1 aataccagcg gaggggttcg atcttgacgt gtttccgggc catgttgaat cccatggcgc
       61 gggtggtttc gacgtcatac cggagggctt cgtcggtggg ggcggtgtag agtccgtcgg
      121 gccaccagcc ctgatcgagg gggcccagct ggaagaggac ctcgttgttg aggaagaggc
      181 ggttgatgcc gcggtcgtct ttggcgacgg cgatcttgcg catgccgaaa tagcttttga
      241 cttcatccag aacctggccg ccgcgcgtga gagtcacggt caaattgtac agaaacggcg
      301 agtcgggcga ccagagccgg gcgttttcga tagggatgtc aaaggcagtg cccggctgcc
      361 cggcgctgcc gaccaccggc gcctcgccgt cgagcgctac cgcgacgatc tcatcgccgg
      421 gggcagtgcc ttctgccgtg gccgtaagac gaagcacgcc tctgtcgatg tcgggggtca
      481 tggccaggtg cgtgatgtgt gcgcggggga cgggctcgag ccagaccgat tgccagatgc
      541 ctgtcacggc ggtataccag atgccgtggg gatcaaggat ctgcttgccg cgcggctgat
      601 agccggtgtc ggtggggtcc cagacggaga cgacgagttc ctgctgctct ccggggatca
      661 gtgcgctggt gatgtcgaaa tagaacggat cgtatccacc ctgatggcgc ccgcagaacg
      721 tgccattcac gtaggcggcg cattcccagt cgaccgcgtt gaagtggagg agcagccgct
      781 ggccctccaa attctccgga agggtgaaac tgcggcggta ccagagggtc tggtcgggct
      841 gtacggcctt tttgacgccg gacagggcgg attcgatggc gaaggggacc aggatctgcc
      901 cttcccaggc tttgggcacg gaggtggctg tgctgggggt gatggcgtag tcccagaggc
      961 cgttgaggtt gtgccaccgg tcgcgggtca tctgaggccg gggatattcg ggccagacgt
     1021 tgtcgggcga tacgtcttca gcccagcggg tcatgagctg gccttcgaca ggcttccatt
     1081 gggccatggc actgcctccc agggtgagcg cgaaaatgag tgtgatggag acggtccgga
     1141 tcgcattcat gtgtattctc cccaacttga cttctggccg ggtgatttgt ttgtacgata
     1201 acaaaacgcg cgatcgttaa ccagatcgca cggcgcctcc ggagaaagga tggtgcgcgc
     1261 tccggaaggg tggtagagtg aggttcactg cgctcatgcg gacgatgcaa cgagagccgt
     1321 tttggtacgt ggagaggaga agcgaacatg cgaggtcggc gggcattcat gcggatggcg
     1381 ttcactttgg cggcggcgct ggttctggcg tgttcggtgc atgcggcgcc acacgacgga
     1441 tttttgcttc gagccggcca gaagctgttt cctctgggtt tttacgaact gccggaaagt
     1501 gacgaaggat tgcaggcgat ggcggacgcc ggggtcaatc tggtgcgctg ccagaatcgc
     1561 catgatctgg accggctcca tgcactggga atgcagggcg tgatgccgtt gcctctggaa
     1621 cagggtccca ccgaagactt tcgcgcccgg gtggcggacg tggcggacca tccggcgctg
     1681 gcggtgtggg agggccccga cgaggtggtg tggaattaca cggcgttcag cggcctgttc
     1741 cgcacgaagg ggatatacaa gacgcgggat gcgtggtgga cgcaggcccc tgaagccagg
     1801 gcgtacgcag cggaacaggc ggcaacgatc gttccgaaca tgcgcgcggc cgtggccctc
     1861 atccgggaac tcgatgcaca acaacggccg gtgtggatta acgaagccaa aaacagcgat
     1921 gtgtattacg tgcggcagta cctggatttt gtggacatca cgggctgcga ctattacccg
     1981 gtgcacgaag atgaacgccg tattgaggag attgtgggcg cgacgcaacg atggaaccag
     2041 atcgggcggg ggatgcccgt gtacatggtg ctgcaggcct tttcctggca cgagctgggg
     2101 gagtattacg gcgcacagcg tgaggcctat ccaacgttcg ctgagtcgcg cttcatggca
     2161 tatgccgcga tcgtgcacgg agcggacggg attctgtatt gggggtcgag ttttctgaaa
     2221 tcggaggcgt gccgggcctc gatctacgcg ctgacgaaag aattggcggc cttgcagccg
     2281 tttttaaccg caccggacgt gatgggcgtg cgcgtgagcg ctatcgaaga ttaccatccc
     2341 gacgtggcgc cgcggatagg ctggatggtg cgccgctcag gcgatgactg gctggtcgcg
     2401 ctggtgaacg aagacgatat acgccatacg gcggtcattg tcgaagggct cgaggcgttg
     2461 aatgggcgga cgctgggcca gttgtatacc gacgacgcgg cggtcgtggc gcgaggagag
     2521 ctgatcgcgc gtatgcagcc gaaagccgtg cacgtgtatg caacaagccg cgattacgag
     2581 gccgactgtg gcg
//
LOCUS       DAOPBQ010000529         2589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_31386_length_2589_cov_3.936069, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000529 DAOPBQ010000000
VERSION     DAOPBQ010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31386_length_2589_cov_3.936069"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKW60_13745"
     CDS             complement(<1..299)
                     /locus_tag="PKW60_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00543.1"
                     /translation="MPHVADSRKKVSRHRRFTPLPRPADTGQAPPPPPPPPYYYQQPV
                     QPQRRTDTNTCLIVGIILGALAFFMIAVMGILAAILLPATFRAREAARRASCANHR"
     gene            431..1732
                     /locus_tag="PKW60_13750"
     CDS             431..1732
                     /locus_tag="PKW60_13750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK00544.1"
                     /translation="MPEPLVYVLVINWNGREHLADCFDSLLSGTYRNCRFLLVDNHSA
                     DGSIAFVRERYRDPRIEILALPDNLGWSEGNNRGIEQALDAGAEYIFLLNNDTATAPD
                     AIEKLVSTAESRPDCGALAPKMVLFDAPHLLNSLGIECSIIGSSWDLGVGRLDGPQWD
                     TPRKVLGVCGGAMFLRSAALRKSGLLPDFQIYLDDLDLCLRIWNAGFEIWNCPGAVVR
                     HKFSATMGAGKRLRQKYYLNTRNRMRLVLRNFPLSRLFLAKPKLLLGEAKSVGRGILD
                     GDYWKLWAHTRAWGASLAYLPEAWRARRRHGTSARFWPLIRQEALFFPGVELPENGWY
                     AERVINGEKLRPISQTAWMRCESGQLRVTLVNCYPALGDAEIEVFASGEVIARLIARE
                     RTEQQLACPSGKIEFRARRIFDAEDTRELCDFGGWLRVERA"
     gene            1783..>2589
                     /locus_tag="PKW60_13755"
     CDS             1783..>2589
                     /locus_tag="PKW60_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPK00545.1"
                     /translation="MAEFDLATYLEERRTFIEDHLDRILPAETEQPCEVSAAMRYGVL
                     RGGKRLRPLMSLMTAEVNGFEHAAVIGAACAIELVHAASLILDDLPSMDNAAMRRGLP
                     CTYVVYGESTAILASMGLIAIAYRLVAENAERLAHPPAPAIALLSKAIGHEGIVGGQH
                     ADLTVRQTRATLEQLTEIYTHKAAALFLAAIRIPGNILAIEPGKLQALETYALNLGLA
                     FQITDDLLDADHAPEDAGRTTFSTHLGMAGTKKRIDEFVTNATAALSVFGG"
BASE COUNT          481 a          804 c          799 g          505 t
ORIGIN      
        1 cggtggttgg cgcagcttgc ccgccgggcg gcttcgcgtg cccggaacgt ggcaggaagc
       61 aggatcgccg caaggatgcc catgacggcg atcatgaaga aggccaacgc gccgagaatg
      121 atgccgacga tgaggcaggt gttggtatcc gtgcgccgtt gcggctgcac gggttgctgg
      181 tagtagtacg gcggcggggg agggggcggc ggcgcctgtc cggtgtcggc cggccgcggc
      241 aacggggtga atctccgatg tctggacacc ttttttctgg aatcggcgac gtgtggcatg
      301 acgcaggccc tcctttccac tggctccccg ctgtagtaca tacactctac actccgggca
      361 tattcactgt ctaccccaag atcaggaccc gattcatcgc gttcccgggc cctgctatac
      421 tgcagccgcc atgcctgaac cactcgtata tgtcctggtt atcaactgga acggccgtga
      481 gcatctcgcg gactgttttg actcgctgct ttcgggcaca taccgaaact gtcgctttct
      541 gctggtggat aaccacagcg cggacggctc catcgcgttt gtgcgggaac ggtaccgcga
      601 cccgcgaatc gagatcctgg cccttccgga caatctgggc tggtcggaag gcaacaaccg
      661 gggcatcgaa caagctctgg atgccggtgc ggagtacatc ttcctgctca acaacgacac
      721 ggccaccgcg cccgacgcca tcgaaaaact cgtgagcacc gctgaatccc gcccggactg
      781 cggtgcgctc gcccctaaaa tggtcctttt tgatgccccg caccttttga attccctcgg
      841 catcgaatgc tccatcattg gctcgtcatg ggacctcggc gtgggccggc tcgacgggcc
      901 ccaatgggat acgccccgca aagtgctggg tgtatgcggc ggcgccatgt tcctgcgcag
      961 tgcggcgctg cgaaaatcgg gattgctccc ggattttcaa atctatctcg acgatctcga
     1021 cttgtgcctt cgcatctgga acgccggttt tgagatctgg aactgtcccg gcgcggttgt
     1081 ccggcacaaa ttcagcgcca cgatgggcgc ggggaaacga ttgcgccaaa aatattatct
     1141 gaacacgcgg aatcgcatgc gcctggtgct gcggaatttt cccctctcaa gactcttcct
     1201 tgccaaaccc aaactcctcc tgggtgaggc caaatccgtc ggacggggca tcctggacgg
     1261 ggattactgg aagctgtggg cccatacaag ggcgtgggga gcaagcctcg cctatctccc
     1321 cgaagcctgg cgcgcgcggc ggcgccacgg cacgagcgcg cgcttctggc ctctgatccg
     1381 ccaggaggcc ctctttttcc cgggtgtcga gctgcctgaa aacggctggt atgccgaacg
     1441 ggtgatcaac ggcgaaaagc tgcgtccgat ctcccaaacg gcctggatgc gctgcgaaag
     1501 cggtcagttg cgtgtcaccc ttgtcaattg ctatcctgcg ctgggtgatg cggaaatcga
     1561 agtcttcgcg agcggcgaag ttattgcgcg gctgatcgcg cgagagcgca cggaacagca
     1621 attggcctgc ccctcgggaa agatcgagtt ccgcgcgcgc cgcattttcg atgcggaaga
     1681 cacgcgcgaa ttgtgcgatt tcggcggctg gctgcgggtg gagcgcgcct gatggcgtcc
     1741 gcttgacgcc ctggacagcg gagcccggaa caggaaaggc gcatggccga atttgacctt
     1801 gccacctacc tcgaagaacg cagaacgttc atcgaagatc atctggatcg catcttgccc
     1861 gccgaaacgg agcagccgtg cgaagtgtcg gccgcgatgc gctacggcgt gctgcgtggt
     1921 ggaaaacgtc tgaggccgct gatgtcgctc atgaccgcgg aagtgaatgg cttcgagcac
     1981 gccgcggtga tcggcgccgc atgtgccatc gaactcgttc acgcggcttc gctcatcctc
     2041 gatgacctgc cctccatgga caatgcggcg atgcgccggg gtttgccgtg cacctacgtt
     2101 gtgtatggcg agtccaccgc catcctcgca tcaatgggcc tgatcgccat cgcctaccgg
     2161 cttgtggccg aaaatgccga gcgtctggcg cacccacctg ccccggcgat tgccctgctt
     2221 tcaaaggcca ttggccacga aggcatcgtg ggcggacagc atgcggatct caccgtgcgt
     2281 cagacccgcg caacgctcga gcaattgacc gagatctata ctcataaggc tgccgccctg
     2341 tttctggcgg cgattcgcat ccccggcaac atcctggcga ttgagccggg caagctccag
     2401 gccctcgaga cctacgccct gaacctgggc ctggcatttc agataaccga tgacctcctc
     2461 gacgccgatc atgcccctga agatgcgggg cggaccacgt tctcgacaca cctcggcatg
     2521 gccggcacga agaagaggat tgatgaattt gtaacaaacg ccacggccgc gctgagtgta
     2581 ttcggcggg
//
LOCUS       DAOPBQ010000530         2580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_31523_length_2580_cov_3.752079, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000530 DAOPBQ010000000
VERSION     DAOPBQ010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31523_length_2580_cov_3.752079"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="PKW60_13760"
     CDS             complement(<1..1514)
                     /locus_tag="PKW60_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00546.1"
                     /translation="MESISLREAAGQAVLLAALLVAAFPGVFFRGEMISPADIAFQQP
                     PWAAYAPADWKGPSNPLMADALTLFRPWYTTTRASVRAGAWPLWNPYEFAGIPLLANY
                     QSAVFYPPRLLHVFLDVDVATTLCILLKLWLAGVAAYLCARTMRLQRPAAVFCSIGWM
                     FSGYNLIWCNWSLPDLSPWLAILVLGSELLFQRRYQRGFFSIALGATLLLLAGHPVSA
                     FTMTLGLGVYFVVRLSLDVRRGQAVLAPLGAWTGAWVLALLVCAALLLPFFEYLGNQY
                     QGHDLAAFGAERGLPLSACAAFWVPRFFGTAADGNYWGELDSNRYSMIYPGVAVWLCA
                     AMALYAWRARRSAGATAIALTAAAAFGIALAFEAYPFSILHGLPFFDAIKRSYHVCFA
                     VFALPVLGAIGLDTWLRETRRSILLSPILLALAACVVVASVWQFNGGLIRMRELTAYL
                     TVQTGLAAGFAVAGLALLASGALFQRKHLFGYALAALLAIDLPLAHRGLNPTIDR"
     gene            complement(1577..>2580)
                     /locus_tag="PKW60_13765"
     CDS             complement(1577..>2580)
                     /locus_tag="PKW60_13765"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPK00547.1"
                     /translation="RDGTLLGITPDGWYEDAAPMSGHEARYSVTARDLLTGHTAEQPV
                     HVALPDFPLPEPPQNITVATRNSRIVLGWEAASPLAAAYRICRYDENDNAVGTVDIAS
                     VPGHFLQYADKVSAGEIYTYEISGVAPDSRVGPPSRRIGVIAAESPIKPMVSLSFEDT
                     TFLAGLAQIAENALALGGSGWAELSPQPEWNPAEQLSVSLWVRMDDLEGMPVLLCKGA
                     WQQSGYFLQIFRGQLRFYIAGVGTLDAGYLKAGEWLHLAATYGFGEMQAYINGELAGC
                     KRVAGRPRPSANPLLVGRYALSEDVYFVRGMLDDIVIHNVCLAPEEIRAIYDESKHD"
BASE COUNT          536 a          824 c          799 g          421 t
ORIGIN      
        1 cggtcgattg tcgggttgag gccacgatgc gccaggggca ggtcaatggc caacaaggcg
       61 gccagcgcgt atccaaacag gtgtttgcgc tggaacagcg cgccggatgc caggagcgcc
      121 aggcctgcca ctgcgaaacc tgccgccagg cccgtttgaa ctgtcagata ggcggtcaat
      181 tcccgcatcc ggatgaggcc gccgttaaac tgccatacgc tcgcgaccac aacacacgcg
      241 gccagcgcca gaaggatggg ggagaggagg atgctgcggc gggtttcgcg aagccaggtg
      301 tccaggccga ttgcgcccag gaccggcagc gcaaacaccg caaaacagac gtggtagctc
      361 cgtttgatcg cgtcgaaaaa cgggagcccg tgaaggatac tgaatgggta ggcttcaaag
      421 gccagtgcga tgccgaaggc ggccgctgcg gtgagtgcga tggccgtagc cccggcactc
      481 cgccgggcgc gccatgcgta cagcgccatg gccgcacaga gccagaccgc cacgcccgga
      541 tagatcatcg agtagcggtt ggagtcgagt tcaccccagt aattgccgtc agcggcggtt
      601 ccgaagaagc gcggcaccca gaatgccgcg catgcgctca aaggcaatcc ccgttccgcg
      661 ccgaaagcgg caaggtcatg gccctggtac tggtttccca ggtactcaaa aaacggaagc
      721 aggagcgcgg cgcagaccag gagcgccagg acccacgccc ccgtccacgc ccccagcggc
      781 gcaagtacgg cctgaccccg gcgcacgtcc agggaaagcc ggaccacgaa gtacaccccg
      841 agtcccaggg tcatcgtgaa cgcggacacg ggatgtcccg ccagcagaag caatgttgcg
      901 ccgagcgcaa tcgagaagaa acctctctgg taccggcgct ggaacaacag ttcgctcccc
      961 aggaccagta tcgccagcca gggactcagg tcgggcaggc tccaattgca ccaaatcagg
     1021 ttgtaccctg aaaacatcca gccgatcgag caaaatacgg ccgccgggcg ctgcaggcgc
     1081 atggttcggg cgcagagata ggccgccacg cccgccagcc agagtttgag gagaatgcag
     1141 agcgtcgtcg cgacatctac atccagaaac acgtgcaaca gccggggcgg atagaatact
     1201 gcgctctggt agttggccag cagcggaatt ccggcaaatt catacggatt ccacagtggc
     1261 caggcgcctg cgcgaaccga tgcgcgcgtg gtcgtgtacc acggacggaa cagggtcagc
     1321 gcatctgcca tcagcgggtt gctggggcct ttccagtcgg ccggagcata ggccgcccag
     1381 ggaggctgct ggaacgcgat gtctgccggg gagatcattt cgccccgaaa aaacacgccc
     1441 gggaatgccg ccaccaggag cgcggcgagg agcacggcct gtcctgccgc ttcccgtaag
     1501 ctgatacttt ccactctggc ggtttcagtg ccctgcatac ggggccaccg ctgtggagat
     1561 cggggctcgt gctgcgtcaa tcgtgtttcg attcgtcata gattgcccgg atctcttccg
     1621 gcgccaggca gacattatga ataacgatgt cgtccagcat gccacgcaca aaatacacat
     1681 cttcgctcaa ggcatagcgc ccgaccagca gcggattggc actgggacgc ggccggcccg
     1741 ccacgcgttt gcagcctgca agctcgccgt tgatgtaggc ctgcatctcc ccaaagccgt
     1801 aggtcgcagc cagatgcagc cattccccgg ctttgagata tcccgcatcc agcgtgccga
     1861 ctcctgcgat gtaaaagcgc aattgccccc ggaaaatctg cagaaaatac cccgattgct
     1921 gccaggcccc cttgcataga agcaccggca tcccttccag gtcgtccatc cgcacccaga
     1981 gcgacaccga aagctgttcc gccggattcc attccggttg cggcgacaat tccgcccagc
     2041 cgctgccgcc gagggccagc gcgttctccg caatctgtgc caggcccgcg aggaaggtcg
     2101 tgtcttcaaa tgagagcgat accatgggtt tgatgggaga ttccgcggcg attacgccga
     2161 tgcgccgtga aggggggccc acgcggctgt cgggagctac accgctgatc tcataggtgt
     2221 agatctctcc tgcggaaacc ttgtccgcat actgcaggaa atggcccggc accgatgcta
     2281 tgtctacggt tccaactgcg ttgtcgtttt cgtcgtatct gcagatgcgg taagccgccg
     2341 ccagcggcga cgcggcctcc cagcccagca caatccggct gttgcgcgtg gcgaccgtga
     2401 tgttctgtgg cggttccggc agcggaaaat cgggaagagc cacatgcacg ggctgctctg
     2461 cagtatggcc cgtcagaagg tcgcgcgcgg taacgctgta acgtgcctca tgtccggaca
     2521 taggcgcagc gtcttcatac catccgtccg gcgtaatccc cagcagcgtg ccgtctcgcc
//
LOCUS       DAOPBQ010000531         2566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_31743_length_2566_cov_3.996814, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000531 DAOPBQ010000000
VERSION     DAOPBQ010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31743_length_2566_cov_3.996814"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PKW60_13770"
     CDS             complement(<1..363)
                     /locus_tag="PKW60_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00548.1"
                     /translation="MIRIHEPFHGAVLNHRHGRKTRNGLEIPVWGAAPAGEAVTVNGA
                     FAQRAGERFFATVTLPGPETDITAAVNSPTGCSEHRIRVVWDRFSKPRYRFAIDDNSF
                     FLRDIAAKRPKSLFQNFYL"
     gene            complement(459..1409)
                     /locus_tag="PKW60_13775"
     CDS             complement(459..1409)
                     /locus_tag="PKW60_13775"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPK00549.1"
                     /translation="MKRQFVETLQEGDRINDYFLAVRKDLRDQRNGGKFLGLMFKDRT
                     GEIGGILWNNAAAVARLFELGDVVNVQGTVTSYQGRLQIRVDQVLPLRTGEFDLADLA
                     YVPETLGTSLAEFIEIMRTVKNEWLARLVSSFLDDEAFMGAFAEAAAGKKWHHAYTGG
                     LVQHCLELAKLADAVCTIFPGVDRDILLTGILVHDVGKIEEMSHGMYVDYTTPGKLLG
                     HLIIGADMVQERIRAIEGFPESLRLQVLHCILAHHGELVNGSPVVPKTVEAMVLHQID
                     NLGAQVDAFLRIVDEARLRGQDWSEYIGLIERPIWTKEGE"
     gene            1550..2554
                     /locus_tag="PKW60_13780"
     CDS             1550..2554
                     /locus_tag="PKW60_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HPK00550.1"
                     /translation="MPKPRTVVLLTREMQASVFSEPHLARLGALVELTPAREDEITPE
                     GQQSAMKGAEIIITGWGTHEISGPMLDAAPDLRLMCHSAGSIKHLISPEFAARGIRVT
                     SAAPALAVGVAEFAFGLMLMSMKAVWACIAATSAGRWEREAVMDWIREPHEATVGIIA
                     ASRVGRELLRLCKNLTLKSILLYDPFVDEEEAAGLGVEKVSLDDLMRRSDVVQLCAPD
                     LPATRHMINAEKLALMRDKAIFINTSRGALVDEAALVAELRKGRILACLDVTDPEPPE
                     PGSPLYTAPNCILTPHIAGAVKQNIRRQGESVLEQIEAYIAGRPMPCEVALDRLDILA
                     "
BASE COUNT          491 a          739 c          826 g          510 t
ORIGIN      
        1 gaggtagaag ttctgaaaca gcgatttcgg gcgtttcgcg gcaatgtcgc gaagaaagaa
       61 gctattgtcg tcgatggcaa agcggtaccg gggcttggaa aagcgatccc agaccacccg
      121 gatgcggtgt tcgctgcagc cggtgggact attgacggcg gctgtgatgt cggtttcggg
      181 cccggggagc gtgaccgtag cgaaaaatcg ttctcccgcg cgctgggcga aagcgccgtt
      241 cacggtaacg gcctcccccg caggcgcggc gccccagacg ggtatctcga ggccgtttct
      301 ggtctttctg ccgtggcggt ggttcagaac agcgccgtgg aacggttcat gaatccgtat
      361 catcaatgta cccttccatt cgtgatttcc ggctaaggcg ctgaggaagt cccggaagtc
      421 tgccggcgcg cggccgcagc ctgcaccacg gagaccgcct actcgccttc tttagtccag
      481 atgggccgtt cgatgagtcc gatgtattcg gaccagtcct gaccacggag cctggcttca
      541 tcaacgatgc ggaggaaggc atcgacctga gcgcccaggt tgtcgatctg atgcagaacc
      601 atggcttcga cggttttagg gactaccggc gacccgttga ccagttcccc gtggtgtgcg
      661 aggatgcaat ggaggacctg caaccgcagg gattcgggga agccttcgat cgcgcgaata
      721 cgctcctgca ccatgtccgc gccaataatg agatgcccca ggagttttcc gggggtggtg
      781 tagtcgacgt acatgccgtg tgacatttcc tcgattttgc cgacatcgtg cacgagaatg
      841 ccggtgagca ggatgtcgcg gtcgactccg ggaaagatgg tgcatacggc gtcggccagc
      901 tttgccagtt cgaggcaatg ctgcacgagg cccccggtgt aggcgtgatg ccatttcttg
      961 cccgcggcgg cttcggcaaa ggcgcccatg aaggcctcat cgtcgaggaa agagctcacg
     1021 agccgggcca gccactcatt tttgaccgtg cgcatgattt cgatgaactc ggcgaggctc
     1081 gtgcccagcg tttccggcac gtaggcgaga tcggcgaggt cgaattcgcc tgtgcgcagt
     1141 ggaagtacct ggtcgacccg gatctggagg cggccctgat aactggtgac cgtgccctgc
     1201 acgttcacga catcacccag ctcgaagagc ctggctaccg ccgctgcatt gttccacagg
     1261 atgccgccaa tctccccggt cctgtctttg aacatcagcc ccagaaattt tccgccgttg
     1321 cgctggtcgc gcagatcttt gcggacggcc agaaaatagt catttatgcg atcgccctcc
     1381 tggagggttt cgacaaactg ccgtttcatg aaatctcctt ctgggagcac ccggcggggg
     1441 cggctccgta tcaattccct agagtatcgt ttgcgtatgc cgccttgcaa atgcccggca
     1501 ccggcggcta ccctgatttc ttcgctgaaa ccgaaggaag gagaccggta tgccaaaacc
     1561 gcgaaccgtg gtgctgctga cgcgcgagat gcaagccagc gttttttctg aaccccatct
     1621 ggcccggctc ggcgcgctgg tcgaactgac gcccgcgcgc gaagacgaaa tcacgcccga
     1681 agggcagcaa tcggccatga aaggcgcgga aatcatcatc accggatggg gaacgcatga
     1741 aatctcgggc cccatgcttg atgcggcgcc ggatttgagg ctgatgtgcc attcggcagg
     1801 ctcgatcaag cacctcatct ctccggagtt cgccgcgcgc ggaatccgcg tgacttcggc
     1861 ggcgccggcg cttgcggtcg gcgtggcgga attcgcgttt gggctgatgc tgatgtcgat
     1921 gaaggcggtc tgggcgtgca tcgccgccac gagcgcgggc cgctgggagc gcgaggcggt
     1981 catggactgg attcgcgagc cccacgaagc aacggtaggc attatcgcgg cgagccgcgt
     2041 gggacgcgaa ctgctgcgcc tgtgcaaaaa cctcacgctc aaatcgatcc tgttgtatga
     2101 cccgtttgtg gatgaagagg aagcggccgg gctgggtgtg gaaaaggttt ccctggatga
     2161 tctgatgcgg cggtcggacg tggtgcagct ctgcgcgccg gatcttccgg caacgcggca
     2221 catgatcaat gctgaaaagt tggcgctgat gcgcgacaag gcgatcttca tcaatacgtc
     2281 ccggggcgcg ctggtcgatg aagccgctct ggtcgcggag ctcaggaaag ggcgtattct
     2341 ggcctgtctc gatgtgacgg atccggagcc accggagccc ggaagcccct tgtacacggc
     2401 tccgaactgc attctcacgc ctcacatcgc aggggcggta aaacaaaata tccggcgtca
     2461 gggggagtcg gttctcgaac agatcgaggc gtatatcgcc ggcaggccga tgccgtgtga
     2521 agtggccctg gatcggctcg atatcctggc ttagccaaag agtgaa
//
LOCUS       DAOPBQ010000532         2564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_31766_length_2564_cov_4.913511, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000532 DAOPBQ010000000
VERSION     DAOPBQ010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31766_length_2564_cov_4.913511"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="PKW60_13785"
     CDS             <1..1279
                     /locus_tag="PKW60_13785"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPK00551.1"
                     /translation="LYSLPPLTCDLFKVDWVDKVETVIKHCNGDEVHVIVAGRVLRGD
                     DGEPMGAVISLTDITSLRRLETEMNQLRREVTGRFEFDNIVGKSPQMREVFNLVELAA
                     ASPVTVLITGETGRGKEVVAKAIHYHSERKNGPLVRVNCSALSESLLESELFGHAKGA
                     FTGAIKDHVGRFERANEGTIFLDEVGDIPPLVQVKLLRVLQERELERVGESIPRKVDV
                     RVIAATHRNLRDLVRRGEFREDLFYRLKVFPLHLPPLRERKEDIDPLVKRFIAQFNAD
                     TGKNILGLTPDALRMVLDYCWPGNVRELENAIEHAFVTCSGAFINPLDLPIEIRRVEL
                     KLQLCDDPSQADAFASPGLYSEFPARRKRGNTREELMAVLEVSGWNKAEAARRLGITR
                     TSVWRRMKKLGVPLERPTGPSRQDIGSGMPHTL"
     gene            1569..1793
                     /locus_tag="PKW60_13790"
     CDS             1569..1793
                     /locus_tag="PKW60_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPK00552.1"
                     /translation="MTVERALRTIAGFFVLASAVLAGVHSRYWLVFTGFVGANLLQSG
                     FTNWCPMVWLLERAGLRRSDGPEEDPESTV"
     gene            complement(2023..2319)
                     /locus_tag="PKW60_13795"
     CDS             complement(2023..2319)
                     /locus_tag="PKW60_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00553.1"
                     /translation="MTEAAHRLKGAQHVTTAQGVPVISATLGLLSYNLASRIPRDAPQ
                     YSNRDANKAEHWGKTALNSDFARNYDQIPHFETQMIQRTACVAQRYKCNDCNFD"
BASE COUNT          490 a          766 c          758 g          550 t
ORIGIN      
        1 cctgtacagc ctgccgccgc ttacctgcga cctgttcaaa gtggactggg tggacaaggt
       61 cgagaccgtg atcaagcact gcaacggcga tgaagtccat gtgattgtgg ccgggcgtgt
      121 gttgcgcggt gatgatggcg aacccatggg cgccgtgatt tcgctgaccg acatcacctc
      181 attgcgacgt ctggaaaccg agatgaacca gctccgccgg gaggtgaccg gccgcttcga
      241 attcgacaac atcgtgggaa aaagcccgca gatgcgcgaa gtattcaacc tggttgagct
      301 cgccgccgct tccccggtga ccgtactcat caccggcgag acgggcaggg gcaaggaagt
      361 agtcgcgaaa gccatccact atcacagcga acgcaaaaac ggtccgctgg ttcgcgtgaa
      421 ttgctccgcc ctgtcggaat ccctccttga aagcgagctg ttcggccacg ccaaaggcgc
      481 tttcacgggc gcgatcaaag atcacgtcgg ccgtttcgaa cgcgccaatg aagggaccat
      541 tttcctcgac gaggtcgggg atattccgcc cctggtgcag gtgaaactgc tgcgggtgct
      601 gcaggaacgc gagctcgaac gcgtcggcga atccattccc cgcaaagtcg atgtccgcgt
      661 cattgccgcc acccatcgca acctgcgcga cctggtccgg cgcggcgagt tccgcgagga
      721 cctcttttac cgcctgaagg tgttcccgct tcacctgccg ccgctgcgtg agcggaagga
      781 agacattgat cccctcgtca agcgtttcat tgcccagttc aatgccgata ccggaaaaaa
      841 tatcctggga ctgacccccg atgccctccg tatggtattg gactattgct ggcccggaaa
      901 cgtccgcgaa cttgaaaacg ccattgagca cgcctttgtt acctgcagcg gcgcgttcat
      961 caatcccctc gatctcccga tcgaaattcg ccgggtcgag ctcaagcttc agctctgcga
     1021 cgatcccagc caggccgatg cctttgcgtc gccagggctg tattcggagt tcccggcacg
     1081 gcgcaaacgt ggaaataccc gcgaggagct catggccgtg ctagaagtct ccgggtggaa
     1141 caaggctgaa gccgcgcggc ggctcggaat cacccgcacg tccgtctgga ggcgcatgaa
     1201 gaagctcgga gtgcccctgg aacggcccac ggggcccagc cgccaggaca ttggctccgg
     1261 catgccccac acgctgtagc cgccgcgtca tagacgtttg catccgcccg ggtttttccc
     1321 tttcctgccg cgacaggctg cgcagaggcc caaaaaatcg ttgcgcaacg ttacaattca
     1381 atcgaaatgt aacgcaaagt aacgttacat tttgctttgc gtttggcatt cgcggtgttt
     1441 tcggggcttc ctaacccgtt taacagcgca tgcttggcct gtaaattgac cttaagtcat
     1501 tttgtggcat ggaaaatgct tacttcccat gcgttgagcg agtcccgtat gggcggagag
     1561 agaagcacat gactgtcgag cgggcgctgc gtaccatcgc agggttcttt gttcttgcca
     1621 gtgcggtgct ggccggggtt cattcccggt attggctcgt cttcacaggg ttcgtaggcg
     1681 ccaatctcct ccagtccgga ttcacgaact ggtgccccat ggtctggctg ctcgaaagag
     1741 ccggtctccg ccggtccgac gggccggaag aggatccaga atctacagtt taatccggtc
     1801 acgatgaccg tcagggttcc ctccctaaaa tcggggcggc ccatccacgc cgccccgtac
     1861 cgccaggtct ttgccgaggc ctgcgcggtg tagctccgcc cgaggaaggt tcgggggctc
     1921 gcgacccctc agtatgccgc gcgggtcccc gaccttcttc acttctccga tgcatgcctc
     1981 cccatttccc gcccttccgc ttatcctgtc tcaggtagtg gtttagtcga aattacagtc
     2041 gttgcatttg taacgttgcg caacgcatgc cgtgcgctgt atcatctggg tttcgaaatg
     2101 aggaatctgg tcgtaattgc gcgcgaaatc agaatttaat gctgttttcc cccagtgttc
     2161 tgctttgttg gcatcgcgat tgctatattg tggggcatcc cggggaatgc gtgacgcgag
     2221 gttgtatgaa agaagcccca gagttgcaga gatcacaggc acgccctggg ctgtagtgac
     2281 gtgttgcgca cccttcaagc gatgcgctgc ctcagtcatt gcggcctgcc tggtgccgca
     2341 gggacgaacg ggctgcctgc ggcgcagcca catccagccg ggcggcacag gcccacccgg
     2401 agaaaacgtt gcgtggccgc ggatgagccg cgaattcacg ctgagaggag gatcctgatg
     2461 gcaatgacgg gactttttgt gttgggtctg ctttgtggag tcctggcggc ggcaatcgcg
     2521 gtagtcttgc tgctgccccg gctgatgttg attactcaga aaag
//
LOCUS       DAOPBQ010000533         2554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_31937_length_2554_cov_3.350940, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000533 DAOPBQ010000000
VERSION     DAOPBQ010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31937_length_2554_cov_3.350940"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PKW60_13800"
     CDS             complement(<1..1128)
                     /locus_tag="PKW60_13800"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPK00554.1"
                     /translation="MKAAVVFENGDAECIRYEEVPAPVPGPGEVLIEVHGAALNHLDI
                     WVRRGRPGVELPVPHILGSDAAGTVLEAGGGVDHVAPGDEVLINPGLSCGACEFCCRG
                     EQSECLSFGIVGMSRPGTFAERVVVPAGNVFAKPPHLNMTEAAALPLAYVTAWRMLMS
                     RAQVRPGETVLIHGIGGGVALAGLQLAHLAGARVLATSSSDDKLMRAREFGADECINY
                     ADNPDLSEAVRDLTDGRGVDIAFDTVGAATWPENFKAVRRGGRTLVDAVDLAAAAGRL
                     TVIVGPNGAGKSTLLRVLSGDIAADAGQVALLDRPLARWRRAEIARRRAVLPQVCALN
                     FSFRVKDVVRLGRLPFPPAAVDREIAARALDGKLRAWSKSPL"
     gene            complement(1174..2340)
                     /locus_tag="PKW60_13805"
     CDS             complement(1174..2340)
                     /locus_tag="PKW60_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPK00555.1"
                     /translation="MAETLHIILDASSALEHPLTGVGYVALHQTSALAALNGGLELRL
                     FGTRARGVALPAEWRQSFTRSFVVPGTRRLKLALWSRWNWPPVEWFCGTADIAHGLFH
                     LLPAAAAARRLVTVHDLVVFRHPETHTAATVCTHRRLLQHAARNADALIAVSESCRQD
                     ILEVLNVPPERVFVVPNGVCIEDFEGAVDPAALARCRQELGLRSDYWIYIGTIEPRKN
                     LTRLLTAYRRIVDRFSDCPDLLLVGRSGWHAEPIFQAIGDLRLENRVFHAGYLARSEV
                     ILLLRNAAACLYPSLYEGFGLPVLEAMAAGVPVLTSNISALPEVIGPCGVLIDPEEPD
                     SLEAGMETLLQQPGACARMAREARERARGFSWENSAQRLAQVYRTVHQDGPWSE"
BASE COUNT          507 a          829 c          791 g          427 t
ORIGIN      
        1 caacggactt ttgctccagg cacggagttt accgtccagc gcccgggcgg caatctcgcg
       61 atccactgcg gcaggcggaa acggcagccg ccccaggcgc accacgtcct tcacccggaa
      121 actgaagttg agggcgcaga cctgaggcaa cacggcgcgg cggcgagcga tctcggcgcg
      181 ccgccaacgg gcgagcggcc ggtcgagcag ggcaacctgc cccgcgtcgg cggcaatatc
      241 gccggacagc acgcgcagca gggtggactt gcccgcgccg ttggggccga cgatcaccgt
      301 caggcgtccc gccgccgccg ccagatcgac cgcatcgacc agggtgcggc cgccgcgccg
      361 cacggctttg aaattctcgg gccaggtcgc cgcaccgacc gtgtcaaacg caatatcaac
      421 cccgcgaccg tcggtaaggt cacgaacagc ttcgctcaaa tctggattgt cggcgtaatt
      481 gatgcactca tcagcgccaa actccctcgc gcgcatcagc ttgtcatccg aggacgacgt
      541 agcaagaacc cgtgcccctg ccaggtgggc aagttgcagg ccggccagcg ccaccccgcc
      601 gccaatgccg tgaatcagga ccgtctcccc cgggcgcacc tgtgcccgcg acatcagcat
      661 gcgccaggcc gtgacataag ccagcggaag cgcggccgct tcggtcatgt ttaaatgggg
      721 cggtttggcg aacacattgc cagcgggaac gactacgcgt tcggcgaagg tgcccggccg
      781 cgacattccg acgatgccga aggaaagaca ttcactttgt tcgccccggc agcagaactc
      841 acatgcgccg cagcttaacc cgggattgat aagcacctcg tcgcccggag caacatggtc
      901 cacgcccccg cccgcctcga gcaccgtgcc cgccgcatcc gatcccagaa tatgagggac
      961 gggcagttcc acgcccggcc gtcctctgcg aacccagata tcgagatggt tcagcgcggc
     1021 gccatggacc tcgatgagga cttcaccggg ccccggtaca ggcgccggta cttcttcata
     1081 ccggatgcac tctgcatcgc cgttttcaaa tactaccgca gctttcacga ctggtctccc
     1141 tggcaccgcc acagctggcg cgcgttgatc tcgttactct gaccatggtc cgtcttgatg
     1201 aaccgtccga tatacctgcg ccaggcgctg ggcgctgttc tcccaggaaa atccgcgcgc
     1261 gcgctctcgt gcttcgcggg ccatccgcgc gcaggcgccc ggttgctgaa ggagcgtttc
     1321 catgccggct tcgagcgagt ccggctcctc tggatcgatt aacacaccac acggcccgat
     1381 tacttcaggg agggcggaga tattcgaggt caagacgggc acacccgccg ccatggcttc
     1441 cagcacaggc aagccaaacc cttcatataa cgacggatag agacacgctg ctgcatttcg
     1501 cagaaggagt atgacctcgg agcgggccag atatccggca tggaagaccc ggttttcgag
     1561 gcgcaaatct ccgatcgcct gaaatatggg ttcagcatgc catccgcttc ggccgaccag
     1621 cagcaggtcc ggacagtctg aaaagcggtc cacgatacgc cggtaggcgg taagaaggcg
     1681 tgtcagattc tttcggggtt cgatggtgcc gatgtaaatc caatagtcgc ttcgcaaacc
     1741 gagctcttgc cggcaccggg ccagggctgc aggatccacc gctccttcaa aatcttcaat
     1801 gcacacgcca ttgggaacga caaagactct ctccggcggc acgttgagga cctccaggat
     1861 gtcctgccgg cagctctcag acaccgcgat cagggcatcg gcattccgtg cggcatgctg
     1921 gagaaggcgg cggtgcgtgc aaaccgtggc cgcagtgtgg gtctcaggat gtcgaaacac
     1981 aaccagatcg tgcacggtga ccaggcgcct ggcagccgcg gcggcgggaa gcaaatgaaa
     2041 caacccatgc gcaatatcag ccgtgccgca gaaccactct acaggcggcc agttccagcg
     2101 ggaccagagc gcgagtttga gccgccgcgt gccgggcacg acaaaagaac gggtgaaact
     2161 ctggcgccat tcggccggga gggcaacgcc tcgtgcgcgc gtgccgaaga gtcggagttc
     2221 caacccgcca ttcagggcag caagggcgga cgtctgatgc agcgcgacgt atcccacgcc
     2281 cgtcaaggga tgctcgagag cgctgctggc gtcgaggatg atgtggagcg tttcggccac
     2341 gacgaccgtt cagtcaactg cgccgcgtat cggaagaggc ggtgtcaccg tcgtccgtca
     2401 cgtcattttc tgttatttcc tggcgctttc tggcggaatc ccggggagta aagttgtgca
     2461 gtgtggaaat ggaaaacggc gtgtcatcgg ctgcagagga attttcgctg ctctgcccgg
     2521 gagcctggtt cgcgggaggg gcggtctcgc cagg
//
LOCUS       DAOPBQ010000534         2543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_32114_length_2543_cov_3.654743, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000534 DAOPBQ010000000
VERSION     DAOPBQ010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32114_length_2543_cov_3.654743"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PKW60_13810"
     CDS             complement(<1..359)
                     /locus_tag="PKW60_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPK00556.1"
                     /translation="MDEFVKGLWRENPVFVMLLGMCPTLAVSSNVQYALAMGACATFV
                     LVMSSVVVSLIRKIVPKEVRIATFIVVIATFVTMVDYVMQYLSLALYQALGAFIPLIV
                     VNCIILGRAEAFACRNNV"
     gene            complement(386..1102)
                     /locus_tag="PKW60_13815"
     CDS             complement(386..1102)
                     /locus_tag="PKW60_13815"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPK00557.1"
                     /translation="MSNSTPAAQKVDQPNSLHMIAVLGGFSFLSGFLIFFVAHATEAG
                     IKQNRLEQLRGAVFQVLPGAVSSQAFAVESDGFRPVEIDDPTPGMKKVYGGYDESGAL
                     VGVAIEVAAQGYQDVIRAIYGYDPGIGRTLGFAVLQSSETPGLGDLIGKDPEFLANFF
                     ASETSQDTLSLELTADGLGLAHAVEFAKSGEKSKPWQIEGISGATISSKAVVRMLNDG
                     SQEIIPLIHAHLGQLQKGSS"
     gene            complement(1099..2169)
                     /locus_tag="PKW60_13820"
     CDS             complement(1099..2169)
                     /locus_tag="PKW60_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPK00558.1"
                     /translation="MTVITPEAFEIRTSPHIKAIVTTDVIMRNVVYALMPVCVFAVWA
                     FGLSALALLCVTTAGTILTEHLVCVVSRKPTTVGDYSAVITGILLALTLPPGLPLWMG
                     FAGGVIAILLGKALFGGLGFNTFNPALVARAFLQAAFPVSLTTWIPAFVDNRFARFIP
                     TSLTLPFLSPNTQAGALADYLAQATIDGWSGATPLSLVKFEGKTTATMDLFTGMVPGS
                     TGETCAIFILLGGAWLVARGMMNWRIAAAMLGSAYLFSWLFYFLDNSSPTPAFMLFSG
                     GLMLGAVFMATDMVASPTTPLGVWIYGGLMGFLTILIRVKGGLPEGVMYAILLGNAVS
                     PHIENFTQPRVFGSERIKKAAK"
     gene            complement(2166..>2543)
                     /locus_tag="PKW60_13825"
     CDS             complement(2166..>2543)
                     /locus_tag="PKW60_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPK00559.1"
                     /translation="RGEDASVVILGGPMMGVSVPSLDIPITKGVTSILVLDKAEVQKR
                     NRKEYPCIRCGSCLDACPMHLNPSRLGQLARKREFETMANSFNLMDCFECGSCSYVCP
                     SNIPLVQRFRVAKTIIRERKAAS"
BASE COUNT          538 a          723 c          800 g          482 t
ORIGIN      
        1 acgttattac ggcacgcaaa ggcttcggcg cgcccgagta tgatgcagtt gaccacaatg
       61 agcggaatga acgcgcccag ggcctgatac agggccaggc tgaggtattg catcacgtaa
      121 tccaccatgg tgacgaaggt cgcaatcact acgatgaatg ttgcgatacg gacctctttg
      181 ggcacgatct tgcggataag cgatacgact acgctggaca tcacgagtac aaacgtggcg
      241 catgcgccca tggccagggc gtactgcacg ttactggata ccgccagtgt cgggcacatg
      301 ccaagcagca tcacgaacac ggggttctcc cgccacagtc cttttacgaa ttcatccatt
      361 gttgtgggtg gattgccctg cgccatcagg agctaccttt ctgcagttgg cccaagtggg
      421 cgtggatgag cgggatgatt tcctgagacc cgtcgttcag catccgcacc accgctttcg
      481 acgagatggt tgcgccggaa atgccttcaa tctgccatgg cttgcttttt tccccgctct
      541 tggcaaattc caccgcgtgg gccagcccga ggccatccgc ggtcaattca agcgaaaggg
      601 tgtcttggct ggtctcggag gcgaagaaat tggccagaaa ttcaggatcc ttgccgatga
      661 ggtcgccaag tcccggcgtt tcgctgctct gcagcaccgc gaatccaagt gttcgcccga
      721 tgccggggtc gtagccgtaa attgcgcgga tgacatcctg gtagccctgg gcggcgactt
      781 cgatagccac gccgacgagg gcgccgctct catcgtatcc gccgtagact ttcttcatgc
      841 cgggggtggg atcgtcgatc tcaaccggcc ggaacccgtc gctttccacg gcgaaggcct
      901 ggctggatac ggcgcccggg aggacctgga agacagcgcc gcgcagctgt tcgaggcggt
      961 tctgtttaat gccggcttcg gtggcgtggg cgacgaagaa aatgaggaag ccggagagaa
     1021 aggaaaagcc gccgagtacc gcgatcatgt gcaggctgtt cggttgatcg actttttgcg
     1081 ctgcgggtgt gctgttgctc atttcgctgc cttcttgatt cgttcgcttc caaatacccg
     1141 gggctgggtg aagttttcaa tgtggggcga cacggcgttg ccgagcagga tggcatacat
     1201 gacgccctcc ggcaatccgc ccttgacgcg gatcaggatg gtcagaaaac ccatcagacc
     1261 accgtaaatc cagacgccaa ggggcgtggt gggagaggcc accatatcgg tcgccatgaa
     1321 cacggctccc agcatcaggc cgccggaaaa cagcatgaac gccggcgtcg ggctcgaatt
     1381 gtccagaaaa tagaacagcc agctgaacag gtaggcggaa ccaagcatcg ctgcggcaat
     1441 gcgccagttc atcatacccc gcgccaccag ccaggcgccg cccagaagaa tgaatatcgc
     1501 gcaggtctcg cccgtggatc ccggcaccat cccggtgaac aggtccatgg tcgccgtggt
     1561 cttgccttcg aattttacga gagacaaggg cgtggcgccg ctccagccgt cgatggttgc
     1621 ttgagcaaga taatcagcca gggcgccggc ctgggtgttc gggctgagga agggaagggt
     1681 cagtgaggtc gggatgaacc gggcgaagcg gttgtcgaca aacgccggaa tccaggtcgt
     1741 cagggaaacc ggaaacgcag cctggagaaa cgcgcgcgcg accagggctg gattaaaggt
     1801 gttaaagccc aggcctccga acagggcctt gcccagaagg atggcgatga cgccgcccgc
     1861 gaaccccatc cacagcggca ggccgggggg cagggtcaag gcgagcagaa tgccggtaat
     1921 caccgcgctg tagtcgccga cggtggtggg tttgcgcgat accacgcaga ccaggtgttc
     1981 tgtcaggata gtgcctgcgg ttgtgacgca aaggagggca agcgcgctca gtccgaacgc
     2041 ccagaccgcg aacacgcata cgggcatcag cgcatatacg acgttgcgca tgatcacgtc
     2101 ggtggtaacg atcgccttga tatgcggcga ggtgcggatc tcgaatgctt ccggggtaat
     2161 gaccgtcacg acgctgcttt cctttcacgg atgatagtct tagcgactcg aaaccgttgc
     2221 accaggggga tattcgaggg gcatacgtaa gagcacgacc cgcattcgaa gcagtccatc
     2281 agattaaatg agttcgccat ggtctcaaac tcgcgtttgc gggccagttg gccgaggcgc
     2341 gaaggattca ggtgcatggg gcaggcatcc aggcagcttc cgcaacggat gcaggggtat
     2401 tccttgcggt tgcgtttctg cacttccgcc ttgtcgagca ccagaatgct cgtgacgcct
     2461 tttgtgatcg ggatgtccag cgaaggcacg gaaacgccca tcatcgggcc gccgagaatc
     2521 accaccgagg catcctcgcc gcg
//
LOCUS       DAOPBQ010000535         2538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_32209_length_2538_cov_4.135320, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000535 DAOPBQ010000000
VERSION     DAOPBQ010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32209_length_2538_cov_4.135320"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PKW60_13830"
     CDS             <1..578
                     /locus_tag="PKW60_13830"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00560.1"
                     /translation="EFDGGNLLNTGPHPLDQALQLFGTDITPRVFCVMRRAVNYGDAE
                     DHVLLVLEGEGRPVINLEISSCCAYPAYTYNVYGTRGGLSGSMGLLEWQYYLPEEAPK
                     LRLQKRPLTNDEGNPAYCSDKLTWHKKTWKAPKSKQDLFNAISDGFYSMLYKTLTQGA
                     PLEVTPMQVRQQIAVIEECQRQNPHIYSAKE"
     gene            complement(753..1304)
                     /locus_tag="PKW60_13835"
     CDS             complement(753..1304)
                     /locus_tag="PKW60_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00561.1"
                     /translation="MKNAKLLKPLALVFIVGAAVIAFGLAGQQALSEGVDGAGNKACG
                     ASSDACCSKAKAAAAAQCERGQTCGEAKAASVEKSADLSTKQGRSEAVWAEYKAAQNA
                     NCEAAKAEGAACSLKAAADKATCPLAAAKAAAVACEGKQACCAELAGSCAGPACEKAA
                     ACEAQKGACPSQQAKAEVAVPSE"
     gene            complement(1477..>2538)
                     /locus_tag="PKW60_13840"
     CDS             complement(1477..>2538)
                     /locus_tag="PKW60_13840"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HPK00562.1"
                     /translation="EGHCVAVADVKSGKEYPATVRNGEFVFVVDSAAPGEVRDFVVKV
                     KDAPAAAAVEIVKQDAADAFDVVIAGTPFTTYTYSNDNRKPYLWPVLAEGGVHVTRDW
                     PMGEKVATDDHVHQKSFWTAYGDINGVDCWGETGDKSGYQHSDAVAGGSGGAYGWISA
                     QNTWQDKDHKPVIAESREYRFYNTPENMKMVDVQVTFTAAYGDAKFADTKEGGIVSLR
                     MHDALREKGGTGAITNAEGLKGMEACWGKPSAWCDYSGALEGAGVRGIAVFDHPANLR
                     HPSCWHVRDYGLMGANCFGLSHFTDGAENGDFTLENGESMTFSYRVFVHSGNVEEARV
                     ADRYADYATPPKAVWADAK"
BASE COUNT          493 a          833 c          699 g          513 t
ORIGIN      
        1 ccgagttcga cgggggcaat ctgctcaaca ccggtcctca cccgctggac caggccctgc
       61 agctcttcgg aacggacatc acgccgaggg tattctgcgt catgcggcgg gccgtcaact
      121 acggcgacgc ggaggatcat gtgctgctgg tgctggaagg agaagggcgc cccgtcatca
      181 atctcgagat ctcatcctgc tgtgcgtacc cggcctacac ttacaacgtc tacggcaccc
      241 ggggaggcct cagcggatcc atgggcctgc tcgaatggca atattacctg cccgaggaag
      301 cgccgaagct tcgcctccag aaacgcccgc tcaccaacga cgagggcaac cccgcctact
      361 gttcggacaa gctgacctgg cacaagaaga cctggaaggc gcccaaatca aaacaggacc
      421 tgttcaatgc gatttcagac gggttctatt cgatgcttta caagaccctg acccagggcg
      481 ccccgctcga ggtcactccc atgcaggtgc gccagcagat cgccgtcatt gaggaatgcc
      541 agcgccagaa cccgcacatc tactccgcaa aagaatagcc cccagcccag cgctccggcg
      601 gcctggcgcg gcaccatggg gcgcgtttcc ggtgaccttc tgtcaggccg catcttccag
      661 acggaaactc aaaaaaagcg ccgccggaga tgaatctccg gcgccgcact cacagtacac
      721 ttctcaacag atgtctacgc gcaaagggga gactattccg acggcacagc aacctcggcc
      781 ttggcctgct ggctggggca ggcgcctttc tgcgcctcgc acgcagcggc tttctcgcaa
      841 gccgggcccg cacaggagcc cgccagctct gcacaacagg cctgctttcc ttcacacgcc
      901 accgctgccg cttttgcggc ggccagcggg cacgtggcct tgtcggctgc ggccttcaag
      961 ctgcacgcag cgccttcggc tttggctgcc tcgcagtttg cgttctgtgc ggccttatac
     1021 tcggcccata ccgcctcact gcgcccttgt ttggtgctca aatcggcgga tttttcgaca
     1081 ctcgcagcct tggcttcgcc acaggtctgc ccacgttcac actgggcagc cgcggccgct
     1141 ttggccttgc tacagcacgc atcggacgaa gccccgcagg ccttgtttcc ggcaccgtcg
     1201 acgccttcac tcaaggcctg ttgcccggcc agtccaaagg caatcacagc ggcacccacg
     1261 atgaaaacaa gagcaagcgg tttcagaagt ttcgcgtttt tcataaattg agatctcccg
     1321 gttaaagtgg tctcgcatgg tttcttttcg tacgttagga aaccattctc atgggcactt
     1381 cattccagct cagggaggct cgcgcccgaa accggaaaaa ccggtcaaat cgctgcaaat
     1441 taacgggttg tcttgcctgt tcgatcggca gtgccgctat ttcgcgtctg cccaaaccgc
     1501 tttcgggggt gtggcgtagt cggcataccg atccgcgacc cgggcttctt ccacgttgcc
     1561 cgaatgaaca aagacccggt aggaaaacgt cattgattca ccattttcca gggtaaaatc
     1621 accgttttcg gcgccatcgg taaagtgact cagcccgaag cagttggcac ccatcagccc
     1681 gtagtcccgc acgtgccagc acgaaggatg gcgcagattg gcgggatgat cgaacacggc
     1741 gataccgcgc acgcccgcgc cttcgagcgc ccccgaataa tcgcaccacg cggaaggctt
     1801 gccccagcag gcctccatgc ctttaaggcc ctcagcattg gtgattgcgc ccgtgccgcc
     1861 tttttcgcgg agcgcatcat gcatacgcaa cgagacaatg ccgccctcct tggtatcggc
     1921 gaatttggca tcgccatacg cagcggtgaa ggtcacctgc acatccacca tcttcatgtt
     1981 ttccggggta ttgtagaaac ggtactcccg cgattccgcg ataaccggct tgtggtcttt
     2041 gtcctgccag gtgttttgcg cggagatcca tccgtacgcg ccgccggagc cgccggccac
     2101 agcatccgag tgctgatagc ccgatttatc ccccgtttcg ccccagcaat ccactccgtt
     2161 gatgtcgcca taggccgtcc agaatgattt ctggtgcaca tggtcgtccg tggcgacttt
     2221 ttcgcccatg ggccagtcgc gggtcacatg gaccccgcct tcggccagca ccggccacag
     2281 gtaaggtttg cggttatcgt tgctgtaggt gtaggtggta aaaggcgtac cggcgatcac
     2341 cacgtcgaag gcgtcggcgg catcttgttt aacgatttcg acggctgctg ccgcgggggc
     2401 gtccttcact ttcacaacga aatcgcgcac ttcaccaggc gccgcgctgt cgacgacaaa
     2461 cacaaattcg ccgttgcgga cggtcgcggg atactctttg ccggatttca catccgctac
     2521 ggcaacgcag tggccttc
//
LOCUS       DAOPBQ010000536         2523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_32474_length_2523_cov_3.821313, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000536 DAOPBQ010000000
VERSION     DAOPBQ010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32474_length_2523_cov_3.821313"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2034
                     /locus_tag="PKW60_13845"
     CDS             <1..2034
                     /locus_tag="PKW60_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00563.1"
                     /translation="ETGVSWPILLTACAEAGSARIHLEIETIAPEDAEVAAFSLPQVL
                     AGDRAFGARKDFALFPGLEYLEGGEPSSSERDLAPPLNDRRVPSAHKIATPLMAVQGE
                     GALVALLWDAQQEWTTGQRRPAARFLAPASDDGLQYTHMSLFAPSVGDYVHENEYEAG
                     DIPFPVKKGQRLHLEACLVLDHQERYAEGSIVHGPHIGGLVLQAMQHWFELFGLPGPG
                     PAPRDWEEEKALCREAYLKSVWHENPAGWSHCRGWDSAPSLGHAVPLMLDLAAGVAPS
                     VVPEIERRIDAAAKRSLQEIGPRNLWTPAACHIMSGEFPFLYGYLPESLKGLKDSAYG
                     RLRGRKDGLWVWNPAGEQHQSLGKPGDHTLGQASGPALHVLRAARLTGDRLLVEQALD
                     AMKQMERYEVPRGAQVWECPLYQPDILAAGQAIRAYCEAYRLTGDPAHLAHARYWAWT
                     GLPFIYMWGLDGVPTMAHNVIAVFGSTFYTHSWIGLPVVWCGLVYAYGLQDLAEFDDS
                     FPWKTIVEGILMSAMRQQYTSGPSKGCYPDSWDIVRNKPNPADINPENILVNGFRLRG
                     QSPEIQCVRFEDADGGPVFLNSPAAISVSDDDAGLREGRLRFTLRDVYAPATHTSLAP
                     VPEPKSVQGAGARVSRSEDMLSVESAWLYDAELQSVILKHSTKGTDITCEILW"
     gene            2048..>2523
                     /locus_tag="PKW60_13850"
     CDS             2048..>2523
                     /locus_tag="PKW60_13850"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK00564.1"
                     /translation="MGSYRNDLFLRAARGERTRRPPIWLMRQAGRTDPEYNALRDETG
                     LPLEKLFRNAELAARISLLPRRIGVDAIIFFQDILTPLAPMGAPFIFRPGPVLEAPFE
                     DASDLETLHEYDVAQELPFVPETIRLTRQALDGEMPVLGFAGAPFTLLTFLLEGGS"
BASE COUNT          470 a          741 c          814 g          498 t
ORIGIN      
        1 gaaaccggag tgtcgtggcc tatccttctg acggcctgtg ccgaggccgg cagcgcgcgc
       61 attcatctcg aaattgagac gatcgcgccg gaagatgctg aggtcgccgc gttttcgctg
      121 ccccaggtat tggcgggaga ccgcgcattt ggtgcgcgga aggactttgc actgttcccc
      181 gggttggaat acctcgaagg aggcgagccg tcgtcgtccg aacgcgatct ggcgccgcct
      241 ctgaacgatc gccgggtgcc gtccgcccat aagattgcga ccccgctgat ggcggtgcag
      301 ggggaaggcg cgctggtggc gctcttgtgg gatgcgcagc aagaatggac cacaggccag
      361 cggcgccccg cggcgcgctt cctggcgcca gccagtgacg acggcctgca atacacgcac
      421 atgtcgttat ttgcaccgtc tgttggcgat tatgtgcacg aaaacgagta cgaagccggg
      481 gatatcccgt tcccggtgaa gaaaggccag cggctgcatc tggaggcctg cctggtcctc
      541 gatcaccagg aacgctacgc ggaaggcagc atcgttcacg gcccgcatat cggtggactg
      601 gtgttgcagg ccatgcaaca ctggtttgag ctgtttggcc tgccgggtcc gggcccggcc
      661 ccgagagact gggaggaaga gaaggcgctt tgccgtgaag cctatttgaa atcggtgtgg
      721 cacgaaaacc cggccggctg gagccactgc cggggatggg atagcgcccc atccctcgga
      781 cacgcggtcc cgctgatgtt ggacctggcg gctggggtcg cacccagcgt tgtgcctgag
      841 atcgagcggc gcattgatgc ggccgccaag cgcagcctcc aggagattgg accacgaaac
      901 ctctggacgc cggcggcatg ccatatcatg agcggagaat ttccatttct gtacggctac
      961 ctcccggaat cgctcaaagg cctcaaagac agcgcgtacg gccgtttgcg gggtcgaaaa
     1021 gacggcctct gggtgtggaa tcctgcggga gagcagcacc agtcccttgg caagccgggc
     1081 gaccataccc tgggccaggc ttccggaccg gcgcttcatg tccttcgcgc cgcgcgtttg
     1141 accggtgacc ggctgctcgt ggagcaggcg ctcgacgcga tgaaacaaat ggaacggtat
     1201 gaagttcccc ggggggcgca agtctgggag tgccccttgt accagcctga catcctggcc
     1261 gccggacagg cgatccgcgc ctattgtgaa gcctaccggc tgacgggcga tcccgctcat
     1321 ctggcgcatg cccggtactg ggcatggacg ggcctgccgt tcatttacat gtgggggcta
     1381 gacggcgttc ccaccatggc ccacaacgta attgccgtat ttggctcgac gttctacacc
     1441 cattcgtgga ttggcctccc cgtggtatgg tgcggtctgg tctatgcgta tggattgcag
     1501 gacctggccg aatttgacga ttctttcccc tggaagacga tcgtggaggg aattttgatg
     1561 tcggccatgc gccagcaata cacaagcggc cccagcaaag gctgctaccc ggattcgtgg
     1621 gacatagtcc gaaacaagcc caatcccgcg gacatcaatc cagagaacat cctcgtgaac
     1681 gggttccggc tgcgcgggca atcgcctgaa atacagtgcg tccgtttcga agatgcggac
     1741 ggcgggccgg tgtttttgaa ttcgcccgcg gcgatatccg tttccgatga cgatgccggg
     1801 cttcgagagg ggcggctgag attcacgctg cgcgacgtgt atgcgcctgc tacgcacacc
     1861 agcctcgcgc cggtaccgga accgaaatcg gttcaggggg cgggggcgcg tgtgtcccgt
     1921 tctgaagaca tgctgtcggt tgaaagcgcc tggctgtatg acgcggaact ccagagcgtt
     1981 atactgaaac attccacgaa gggaaccgat atcacgtgcg aaatcctgtg gtaggcggaa
     2041 ctgcgtgatg gggtcatatc gaaacgatct ctttttgcgt gccgcccgcg gagaacggac
     2101 ccggcgtccg ccaatctggt tgatgcggca ggcagggcgg acagatcccg aatacaacgc
     2161 attaagggat gagacggggc ttccgctgga aaaactgttc cgcaatgcgg aattggctgc
     2221 gcggatatcc ctgctccccc ggcgtatcgg tgtcgatgcg attattttct tccaggatat
     2281 tctcacgccg ctcgccccca tgggtgcacc gttcatcttc cggcctgggc ctgttctgga
     2341 agctccattc gaagacgcga gcgatcttga gacgttgcac gaatacgacg tggcgcaaga
     2401 gcttcctttt gtcccggaga ctatccggct cacacggcag gcactcgacg gcgaaatgcc
     2461 ggtgctcggg ttcgctggcg ctccgtttac actgttgacc ttcctgctcg aaggtggcag
     2521 ctt
//
LOCUS       DAOPBQ010000537         2498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_32909_length_2498_cov_3.527630, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000537 DAOPBQ010000000
VERSION     DAOPBQ010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32909_length_2498_cov_3.527630"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2191
                     /locus_tag="PKW60_13855"
     CDS             <1..2191
                     /locus_tag="PKW60_13855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK00565.1"
                     /translation="RRVSEFVINHRVRGQERHFAVSGTPIFDRRGRHAGTVSIFRDVT
                     EQAELSKSLEKYAAGLQRLVEGQTRQLRESAERLRGLLIHMNEGFLTLDSAYRVRFSN
                     ERICEMLGLEPGELVGRDIFEFVDAAGRAKLLNLLEVAESKQPRRLQQETSLISSARG
                     GLPVVLGVSRVEDSREGGARYSLVVTDVSKQKEMQTQLEIRAIQLESTNEELRILDRT
                     KDSFLTNVTHELRTPLSTIRGYVEMLDSECIGALENEQKNALEVISRNLERLGTLIEE
                     MIEFSRMQIRGFRLRLSLFNLPELAEECVRSIRPAAQQHGLSLVFSPPEGLPQVWGDR
                     ARLAQVLTNLLSNAVKFTGSGGKIQLNLSRAPGGTALLSVVDTGIGIAPEFQKRVFDK
                     FFQVDNSLERRYEGAGIGLSIAKSIAEAHGGTIELFSAPGEGSRFTLVLPEVFFDDGY
                     TPGEEARFYEKRIAWVSDDSDLREKLAVLLEAWGARVDRISGGHEAIRACRENPPDLV
                     ISDENTAGLAGPAFVALLHNALGEQAGPVILLKGRNTPEEEYIVLSGMVETLEKPFQP
                     HEFFWKVHRMLSDPHSLLALSEARPPASRDFRGTMGCAVIVPDEDLLDWLHTALANRK
                     IAASYHSDPDDALAQLETRGARSLLIDMDGLTPRQSERLAQWVQASHTPLCAITGLPE
                     GSPSVHMADVILAKPFNIQDVLDAFSELDAAHAAIGGAAENYHESAP"
BASE COUNT          504 a          730 c          773 g          491 t
ORIGIN      
        1 ccgccgggtg tcggaattcg tgatcaacca tcgagtgcgg gggcaggaac gccatttcgc
       61 cgtaagcggc acccccatct ttgatcgccg gggacggcat gccggcaccg tatccatttt
      121 tcgtgatgtg accgagcagg ccgagctgtc gaagagcctg gagaaatacg ccgccggcct
      181 gcagcggctg gtggaaggcc agacccggca gcttcgcgag tcggcggagc ggctccgcgg
      241 cctgctgatt cacatgaatg aagggttttt gacgctcgac agcgcttatc gagtgcgatt
      301 ttccaatgag cgcatctgtg agatgctggg gctggaaccc ggagaattgg tcggccgcga
      361 catttttgag tttgtggatg ccgcgggccg ggccaagctg ctcaacctgc tcgaagtggc
      421 cgaatccaaa cagccccggc gtcttcagca ggaaacctca ctgatctcaa gcgctcgcgg
      481 cgggctcccg gtcgtgctgg gcgtctcccg cgtcgaggac tcgcgcgaag gtggcgcgcg
      541 ttactccctg gtggtcacgg acgtgagcaa gcagaaagaa atgcagaccc agcttgaaat
      601 tcgggcgatc cagctggaat cgaccaacga ggagctgcgg atcctggacc gcacgaaaga
      661 cagttttctg accaacgtga cccacgaact gcgcaccccc ctgagcacca tccgtggcta
      721 cgtggagatg ctcgactccg aatgcatcgg cgctcttgaa aacgaacaga aaaacgccct
      781 ggaagtcata tcgcgaaatc tcgaacgcct cggaacgctt atcgaagaaa tgatcgagtt
      841 cagccggatg caaatccggg gcttccgcct gcggctatcc ctgttcaatc tgccggagct
      901 tgccgaagag tgtgtacgat ccatacgccc ggcggcccag caacacggcc tgagccttgt
      961 gttttctccc ccggaaggcc tgccccaggt ctggggcgat cgcgcccgcc tggcgcaggt
     1021 tcttacaaac cttctgtcca atgcggtgaa attcacgggg tctggcggga agatccagct
     1081 taatctcagc cgcgcgccgg gtggaacggc cctcctgagc gtggtagata ccggtatcgg
     1141 gatcgcgccg gaatttcaga aacgtgtgtt tgataaattc tttcaggtcg ataactcact
     1201 ggaacgccgt tatgaggggg caggcatcgg cctctcgatc gccaaaagca tcgccgaagc
     1261 gcacggcggg accatcgaac tcttcagtgc tccgggcgaa ggcagccggt tcacgctggt
     1321 gttgccggag gtctttttcg acgatgggta tacccccggc gaagaagccc ggttttacga
     1381 gaaacgcatt gcctgggtgt ctgatgactc tgatctccgg gaaaagctcg ccgttctcct
     1441 ggaggcatgg ggcgcacggg tggacaggat ctcgggcggg cacgaagcca ttcgcgcgtg
     1501 cagggaaaac ccgcccgacc tggtcatttc agacgaaaac acagccggcc tggctgggcc
     1561 ggcattcgtc gcccttctcc acaatgcgct cggcgagcag gctggcccgg ttattctgct
     1621 taaaggaagg aacacgccgg aagaggaata tatcgtcctg agcgggatgg tcgagacgct
     1681 ggagaaaccg ttccagccac atgagttctt ctggaaagtg catcggatgc tcagcgatcc
     1741 gcacagtctc ctggcgctgt ccgaagcccg gccgccggcg tctcgagact ttcggggaac
     1801 catgggctgc gctgtgatcg tgcccgatga agatctgttg gattggctcc ataccgccct
     1861 ggcgaaccgg aagatcgccg cttcgtatca cagcgatcca gacgatgccc tggcccagct
     1921 tgagactcgg ggggcccgct cgctcctgat cgatatggat ggactcacgc cccggcaatc
     1981 cgagcgcctt gcacagtggg tgcaggcatc ccatacaccc ctgtgcgcga ttacaggact
     2041 gcccgaaggc agtccgtccg tgcatatggc cgatgtcatc ctggccaagc cgttcaacat
     2101 tcaagatgtg ctggatgcat tttcggagtt ggatgctgca catgccgcca tcggcggcgc
     2161 agcggagaat tatcatgaaa gcgccccatg agccgtttgg ctctgtcaca aatggtgaat
     2221 cttgagatct ccatgcaggc tgacgcaggc ctcctcggag aagtgcaggt agacagggga
     2281 gacatggtgc aggcacggtc ccaggcatgc ctctgtgtct cccgtcaaac gttgaaatta
     2341 ttgaagcagt accggaggac actttaaatc atgaaggcgg gcctatccat tgttctgttt
     2401 gtatgtgcac tggcgccggc ggcctatgcc ggcctgaacg agcagcgcct ggcggaatgc
     2461 aggagcgcgg tctgcatcct tgcggattcc gggcttcc
//
LOCUS       DAOPBQ010000538         2491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_33036_length_2491_cov_3.955665, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000538 DAOPBQ010000000
VERSION     DAOPBQ010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33036_length_2491_cov_3.955665"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..508)
                     /locus_tag="PKW60_13860"
     CDS             complement(104..508)
                     /locus_tag="PKW60_13860"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HPK00566.1"
                     /translation="MDSIRRFRNVALGCGLLLSVAAAAGACVFSPALGQGLLCGGIAS
                     VLAFWLLLRQTERLAGAPNAKAHAAIYVWTPLRLLLYGAVLIAGYRLDPESLHGLLGA
                     AIGLFITQGVVLVLGLAWSGPKISPDQDEQPR"
     gene            775..2394
                     /locus_tag="PKW60_13865"
     CDS             775..2394
                     /locus_tag="PKW60_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014023001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK00567.1"
                     /translation="MAAVWFAGGCATMKPPQYEADVVVYGGTSGGVAAAMQAVRLGKT
                     ALIAGPDVHLGGLSSSGLGWADTGRKEAIGGIAREFYERVAEHYADDASWRYQSHEEY
                     IRLRGGRHDPHEGAMWVFEPHVAERIYEDFVSECGIPVYRDEWLDRESGVTVDRGQIG
                     AIRMTSGKVFRGKVFIDATYEGDLMAAAGVNYTVGREANSVYGETLNGVQTQNAKYHQ
                     FDEPVSPYVVPDDPSSGLLPRVHDEGPGEEGSGDHRIQAYCFRMCLTKVPENRVAFTR
                     PEDYDVTQYELLARLLAAGWETVFRKFDPIPNAKTDTNNYGGFSTDNIGMNYDYPEAS
                     YERRAEIIREHERYQKGLMWFLANDPRVPPAVQEEMRQYGLARDEFLDNNHWPHQIYV
                     REARRMVSDFVMTERHLRRQEPTPRPIGMGSYNMDSHHVQRYVVHVENGGDYVLNEGD
                     VQVSPGGSYPISYGAIVPKESDCTNLLVPVCLSSSHIAYGSIRMEPVFMILGQSAATA
                     AVQAIDARCTVQQIDYDRLRERLLQDGQVLE"
BASE COUNT          493 a          759 c          819 g          420 t
ORIGIN      
        1 ggttgcctgg aagtctcgct ggacgcgacg gacatccgtg cattgacggg ctcaaaacgc
       61 ctgctcgact gggaatgacg cagcccggcc cgcgtgcgcg ggttcagcgt ggctgctcgt
      121 cttgatccgg gcttattttc gggccggacc aggccaggcc gagcacgagc acgacgccct
      181 gggtgataaa gaggccgatc gccgcgccca acaagccatg cagcgattcg gggtccagcc
      241 ggtatcccgc gatgagaaca gcgccgtaca gcagcagccg taacggggtc cagacgtaga
      301 tggcggcatg ggcttttgcg ttcggcgcac cggccagacg ctcggtttgc cgcagcagaa
      361 gccagaaggc cagcacgctc gcgatgccgc cgcagagcag tccctggccc agggcaggag
      421 agaacacgca ggcgccggct gccgccgcta ccgacagcag cagcccgcag cccagggcca
      481 cgtttcggaa ccgccggatc gagtccacgc cgcctccttt gcgggctgaa agaacggtct
      541 ccgaagccgt tctggagccc gtattcgctg tgcaggggat tatacccggc acggcacgcc
      601 ggtggcagcg tcaggcagct caaagatgac aaccggatcc ggggatcata tgatgccatt
      661 ctgcaccggg caggcgtgct gccccgtggc actgcgggaa tgcaatcgcg gattgacggc
      721 cgggaggaaa tgacggttat ggcacgaagc ttcggattga gcattggcct ctggatggcc
      781 gcggtctggt ttgcgggagg gtgcgctacc atgaaacctc ctcagtacga ggcggatgtg
      841 gttgtgtacg ggggaacgtc gggcggggtt gcggccgcca tgcaggccgt gcgcctgggc
      901 aaaacggcgc tcattgccgg acccgacgta cacctgggcg gcctgtcgtc aagtggtctg
      961 ggttgggccg ataccggccg gaaagaggct atcggcggca tcgcgcggga attctatgag
     1021 cgtgttgccg aacactatgc ggatgacgcg tcgtggcgct accagagcca cgaggaatat
     1081 atccggctgc gcggcggccg gcacgatccc cacgaaggtg cgatgtgggt cttcgaaccc
     1141 cacgtggccg agcggatcta cgaagatttc gtgagcgaat gcgggatccc ggtttaccgc
     1201 gatgaatggc tcgaccgcga atccggtgtt acggtcgacc ggggccaaat cggcgccatc
     1261 cgcatgacca gcgggaaggt gttccggggg aaggtcttta tcgacgcgac ctacgaaggc
     1321 gacctgatgg ccgcagccgg cgtgaattat accgtcggac gcgaagccaa cagcgtgtac
     1381 ggcgagaccc tcaacggtgt gcagacacaa aacgcgaagt atcaccagtt cgacgagccg
     1441 gtgtcgccgt atgtggtgcc tgatgatcct tcgagcggac tgctgcctcg ggtgcacgac
     1501 gaaggtccgg gggaagaagg atcgggcgat caccgcatcc aggcctactg tttccgcatg
     1561 tgcctgacca aggttcccga aaaccgtgtg gcgttcaccc ggcccgagga ttacgacgtg
     1621 acgcagtatg aactgcttgc gcgcctcctg gccgcgggct gggagacggt gttccgcaaa
     1681 tttgatccga tccctaacgc caagaccgac accaacaatt acggcgggtt ctcgaccgac
     1741 aatatcggca tgaactacga ctacccggaa gccagctacg agcgccgcgc cgagatcatc
     1801 cgcgaacacg agcgctatca gaaggggttg atgtggttcc tcgcaaacga tccgcgcgtc
     1861 ccgcccgcgg ttcaggagga aatgaggcaa tacggcctcg ccagagacga atttctcgat
     1921 aacaaccact ggccgcacca gatctacgtg cgcgaggccc ggcgcatggt cagcgacttc
     1981 gtcatgaccg agcggcacct ccggcgccag gaacccacgc cgaggcccat cggcatgggt
     2041 tcctacaata tggactcgca tcatgtgcag cgctatgtcg tacatgttga aaacggcggc
     2101 gattacgtgc tcaacgaggg tgacgtgcag gtcagccccg gcggctcgta tcccatctct
     2161 tacggcgcta tcgtgccgaa agagagtgac tgcacgaatc tgctggtccc ggtgtgcctg
     2221 tcctcgtcgc acatcgccta cggctcgatc cgcatggagc ccgtattcat gattctcggc
     2281 caaagcgccg ccacggccgc cgtgcaggct atcgacgccc ggtgcacggt ccagcagatc
     2341 gattacgacc ggctccgcga gcggctcctt caagacggcc aggtgctgga atgagcaggc
     2401 tccagcgccg gccggttgca ccgttttgga ggctcggcat acaatcaatc gagagaaggg
     2461 cgccgcattg cgggcaacgg gagttccatg a
//
LOCUS       DAOPBQ010000539         2477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_33296_length_2477_cov_3.571841, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000539 DAOPBQ010000000
VERSION     DAOPBQ010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33296_length_2477_cov_3.571841"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PKW60_13870"
     CDS             complement(<1..218)
                     /locus_tag="PKW60_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00568.1"
                     /translation="MTQGQPGVGAAYANVCKFGVDGIFLRKRTQDRFSGVRFHCGEEL
                     ESVPEKKQGEQEQQGAQKAHNPQICLSR"
     gene            356..1693
                     /locus_tag="PKW60_13875"
     CDS             356..1693
                     /locus_tag="PKW60_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00569.1"
                     /translation="MRISVIPGIALVLVAALLAPPAYAASGLQWFRGQMHTHTYWSDG
                     RAFPEQAIEAYKERGYHFLCLSDHNRFESNTDYWCQLAAEEGGWPPKVTHEMFDAYVL
                     SCGEDWVETRTEGDNTFVRIKTYAEVKPRYEEPGQFLLIPGVEITQTLNGYAIHQNYI
                     NIPAVLPSIVGAPLSQKLEGEMTPAELLQQNSTEARQTAAELQVPYLYMVNHPFWVYY
                     DVVPQNLLDCPEIRFFEICNGGSEFAPHPQAESYTPDKFWDVVNAFRRIQGQDFLYGV
                     GSDDAHFYDVKRIDQNDGVGDAWVMVRAQSLTPEHLLAALNQGDFYATTGVLLDDVAF
                     TPADKTLRVKVKPEEGATYRIHFITTKQGFDPTMTEVASPAEERRPARNVPVYSEDIG
                     RVVKTVDGTEAEYTLEEGDLYVRARIESNIPGKFTRHFHPDTLTAWTQPYAAE"
     gene            1867..>2477
                     /locus_tag="PKW60_13880"
     CDS             1867..>2477
                     /locus_tag="PKW60_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HPK00570.1"
                     /translation="MSCRSPAFDTDKYLAEQSAEILNRMRQFDNKLYLEFGGKLVFDY
                     HAARVLPNFDPNVKVRLLQQFKDKTDIILCIHAADIDHRKMRADFGITYDIDVLKLID
                     DLRREWDLEITAVVITRYQEQPSAQSFKNLLERRGIRVYTHRATKGYPTDVDVIVSEE
                     GYGSNSYIETSKPLVVVAGPGPGSGKLGTCLSQLYHEHRRGVQA"
BASE COUNT          554 a          715 c          667 g          541 t
ORIGIN      
        1 tcacgggaaa gacatatctg tggattatgc gccttctggg cgccctgctg ttcttgttcg
       61 ccttgcttct tctctggaac gctttcgagt tcttcaccgc agtgaaatcg cacgcctgaa
      121 aagcgatctt gagtcctttt tcgcagaaaa atgccgtcta caccaaattt acagacgttg
      181 gcgtacgcgg cccccacgcc gggttgacct tgagtcatgg tgcatggtca aatgctcaga
      241 acaagatcta gcgtgctgaa gttgctgtgg cgcgacatac gcggtatttc cgcaggagac
      301 gtttccaggg ctgccgcgaa ggagaaggag tgagaccatg acaaagaaga tcagaatgag
      361 aatctcagta attccgggca ttgcccttgt cctcgttgca gccctcctgg ctccacccgc
      421 atatgcggcc agcggcttac agtggttccg gggccagatg catacccaca cgtactggtc
      481 ggacggccgg gcctttcccg aacaagccat cgaagcttac aaagagcgcg gctaccattt
      541 cctgtgcctc tcggaccaca atcgctttga gagcaacacc gactactggt gccagctggc
      601 agctgaagaa ggcggctggc cgccgaaggt cacgcatgag atgttcgatg cctacgtgct
      661 gtcatgcggc gaggactggg tggagacgcg aaccgaagga gacaacactt ttgtccgcat
      721 caaaacctat gccgaagtga aaccgcgtta cgaagagccc ggtcaattct tgctcatacc
      781 tggagtcgag attacccaga cgttgaacgg ttatgccatc catcaaaact acatcaatat
      841 ccccgctgtc cttcccagta ttgtgggtgc tcccctgtcg caaaaactgg aaggcgaaat
      901 gaccccggct gaattgctgc agcaaaattc taccgaagcc cgtcaaaccg ccgcggaact
      961 ccaggtgccc tatctctaca tggtcaacca tccgttctgg gtttattacg atgttgtccc
     1021 gcagaacctg ctcgattgcc ccgaaatccg cttctttgag atctgtaacg gcggttcgga
     1081 gtttgccccg catcctcagg cggaatccta tacgcccgat aaattctggg atgtggtcaa
     1141 tgcgtttcgc cgtattcagg gacaggactt cctctacggt gttggctcgg acgatgcgca
     1201 tttctacgat gtgaagcgca ttgatcaaaa tgacggagta ggagatgcct gggtcatggt
     1261 gcgcgcgcaa tccctgaccc cggaacacct gctcgccgcg ctgaatcaag gcgattttta
     1321 tgccaccacg ggcgtgctgc tggatgacgt ggcctttaca cccgcggata agaccctgcg
     1381 ggtcaaagtc aagccggaag aaggcgccac gtaccggatc catttcatca ccacgaagca
     1441 gggcttcgat ccgaccatga ccgaggtcgc cagtcccgcc gaagaacgcc gtcctgcccg
     1501 caacgttcca gtttactcgg aggatatcgg acgtgttgtc aagaccgtgg atggcaccga
     1561 ggccgaatat actctggagg agggtgacct ctatgtccgg gcgcgcatcg agtcgaatat
     1621 ccccggaaaa tttacgcggc atttccaccc ggatacactg accgcgtgga ctcagcccta
     1681 cgcagccgaa taggcacaga atgtagagcc ctgccccttg gaatccggtg gcgcttccgg
     1741 gcgtaacagt acttttcttg tggccggaat ggtgaactgt gccggtatgg ccagcattga
     1801 atccgcggat cccgtggcgc taccatacgg cgggtccgtg gcgatgagga gattccgcca
     1861 ctcatgatgt cctgcagaag tccagcgttt gatacggata agtacttggc tgagcaatcg
     1921 gccgagattc tcaaccgtat gcgccagttc gacaacaaac tttacctgga gttcgggggg
     1981 aaactcgtct ttgattacca tgcggcccga gtgcttccca acttcgaccc caatgtcaaa
     2041 gtccgtctcc ttcagcagtt taaagataaa accgacatca tcctctgtat tcatgccgcc
     2101 gatatcgacc accgcaaaat gcgggccgat ttcggcatta cctacgatat tgatgtcctc
     2161 aaactcatcg atgatttgcg ccgggaatgg gatctcgaaa ttaccgctgt cgtcatcacc
     2221 cggtaccagg agcaaccctc cgcccagtcg ttcaagaact tgctcgaacg gcgtggcatc
     2281 cgggtgtata cccatcgcgc tacaaaaggg taccccaccg atgtggacgt tatcgtcagt
     2341 gaggagggtt acggcagcaa ttcatacatc gagacttcca aaccgctggt ggtggtcgcc
     2401 ggacccggac ccggaagcgg caagcttggc acctgcctgt ctcagctcta ccatgagcat
     2461 cgccgcggtg tgcaggc
//
LOCUS       DAOPBQ010000540         2456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_33649_length_2456_cov_2.838817, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000540 DAOPBQ010000000
VERSION     DAOPBQ010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33649_length_2456_cov_2.838817"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKW60_13885"
     CDS             complement(<1..265)
                     /locus_tag="PKW60_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00571.1"
                     /translation="MAGTVVLGILILGCGIALIAAVQRAIVFPATRGMFWNPADAGWA
                     YRDVVLHVNGYTTHGWFIPVPRSRGAILYSHGNGGNISGRQPGL"
     gene            414..1769
                     /locus_tag="PKW60_13890"
     CDS             414..1769
                     /locus_tag="PKW60_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPK00572.1"
                     /translation="MTEYNILLDSKKMPDAWYNVTPDLPAPMQPPLHPATHQPLKAED
                     LEAIFPRSLIEQEMSVQPAISIPGEVMDIYRLYRPTPLRRAYRLEKMLDTPARIFYKN
                     ESVTPSGSHKINTSIAQAYFNKKDGVNELTTETGAGQWGTALSIACAMLGMKCTVYMV
                     RVSFDQKPYRRVLMQTYGADVVSSPSDTTDSGRSFQAKFPDTTGSLGMAISEAVEVAA
                     KSGGRAKYSLGSVLNHVLLHQTVIGLEAKAQLEQVGEEPDVVIGCCGGGSNFAGIAFP
                     FVPMKKNGKDIDFIAVEPTACPTLTRGLYAYDYGDTAHLTPLLLQYTLGHQFVPPGIH
                     AGGLRYHGVAQQVALMVKEGFMRAVACHQNPVFEALKMFSVAEGILAAPESGHAIRVA
                     IDEALKCKESGESRAILFNLSGHGHFDMTAYEAYASSKLEDYDLDQSIIDAAEAVIPK
                     I"
     gene            1946..>2456
                     /locus_tag="PKW60_13895"
     CDS             1946..>2456
                     /locus_tag="PKW60_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPK00573.1"
                     /translation="MNKTPIRSCCAALLLAALPALLTGCPKPLFSENVVRTSDLVYGA
                     GYVKDNTTELGYRLKDLHFDLVEPTDGPATLRPAVLMIHGGTFLTGSKNDENLVRAAN
                     GLATEGYVCFLIDYRLDGDNPPVEKSWQPDTEDTKDLNIPSLAAVRAAFVDAKTAMRH
                     IRANSSTYGI"
BASE COUNT          539 a          762 c          690 g          465 t
ORIGIN      
        1 caagcccagg ctggcggccg ctgatattgc caccgttgcc gtgtgaatag agaattgcac
       61 cgcgcgaccg gggcaccggg atgaaccagc cgtgcgtggt gtaaccgttc acgtgcagaa
      121 cgacgtcacg gtacgcccat cccgcgtccg caggattcca gaacatgccg cgcgtcgcag
      181 gaaagacgat agcgcgctgc actgcggcaa taagcgcaat cccgcatccc aaaatcaaaa
      241 ttccgaggac aacagtcccg gccatccgtc tcctcccgct cattcgcagc agcccttccc
      301 ggggaacagc cattcgcctg tctcaggggt ggttttttac tcgcatccta tcgtttgaat
      361 attgtatgat taggattcgc aattcaacga agacccagga gggggagcga gcaatgaccg
      421 agtacaatat tctacttgat agcaaaaaaa tgcccgatgc gtggtacaac gtcacgccgg
      481 acctgcctgc gccgatgcag ccgccgcttc atccggctac ccaccaacca ttgaaggccg
      541 aggatctcga agcgatcttc ccgagatcgc tcattgagca ggaaatgtcc gtccaaccgg
      601 caatctctat cccgggcgag gtgatggaca tctaccgcct gtatcgcccc acgcctttgc
      661 gccgtgcata ccgcctggaa aagatgctcg acacgccggc caggatcttc tacaaaaatg
      721 agagcgtcac cccgtccggc agccataaaa tcaacacctc gatcgcccag gcctatttca
      781 acaagaaaga cggcgttaac gagcttacaa ccgaaaccgg cgccggccaa tggggcaccg
      841 ccctctccat cgcctgtgcg atgctgggca tgaaatgcac cgtgtacatg gtgcgtgtca
      901 gcttcgatca aaagccgtac cggcgcgttt tgatgcagac ttacggcgcc gatgtcgtct
      961 ccagcccgag cgatacaacg gacagcgggc ggtcatttca ggcgaagttt ccggatacga
     1021 caggcagcct gggcatggcc atctccgaag ccgtcgaggt ggcggccaag agcggcggcc
     1081 gggccaagta cagcctggga agcgtgctta accacgtgct tctccaccag accgtcatcg
     1141 ggctggaagc gaaagcccag ctggagcagg ttggcgaaga gcccgatgtg gtaatcggct
     1201 gctgcggcgg cggttcaaac ttcgccggca tcgcattccc cttcgtaccc atgaagaaga
     1261 acggcaagga catcgacttc atcgcggtcg agcccaccgc ctgcccaacc ctcacccggg
     1321 ggctgtatgc ctacgattac ggcgacaccg cacacctgac tccgctgctg cttcagtaca
     1381 ctctgggcca tcagttcgtt ccgccgggaa ttcacgccgg cggcctccgc taccatggcg
     1441 ttgcccagca ggtggctctc atggtcaagg aagggttcat gcgcgccgtg gcttgccacc
     1501 agaatccggt gtttgaagcc ctcaagatgt tctccgtggc ggaaggcatt ctggcggcgc
     1561 ccgaatccgg gcacgcgatc cgcgtcgcca ttgacgaggc gctcaagtgt aaggaatccg
     1621 gagaatccag ggccatcctc ttcaacctga gcgggcacgg tcactttgac atgaccgcgt
     1681 acgaggccta cgccagcagc aaactggaag attatgatct cgaccagagc atcattgatg
     1741 cggcggaggc cgtaattccc aagatctgac cattcaataa catcgtgagc cggggagagt
     1801 tcgctgggac aggggactct ccctttttat atggccgagg cgcggtcatt gtcgtgcgag
     1861 tgcggcgcgc agggctgaaa gaggttatac ttggagtaaa taagcctgtc ccgtgatgat
     1921 ctgaggagcc ttcgggagag ccaccatgaa caaaacgccc atccgcagtt gttgcgccgc
     1981 cctgctgctt gcagccctgc ccgccttgct caccggttgt ccgaagccgc tgttttcgga
     2041 gaacgtcgtc cggacaagcg acctcgtgta tggcgcgggc tacgtgaaag ataatacgac
     2101 tgagctcggc taccgtctca aggatctcca tttcgatctg gttgagccta ccgacgggcc
     2161 ggccaccctc agacctgccg tcttgatgat ccatggcggc actttcctca ccggctcaaa
     2221 aaacgacgag aacctcgtgc gcgcagccaa tggacttgcc acggaaggct atgtctgttt
     2281 tttgatcgat taccgcctgg atggcgacaa ccctcccgtt gaaaaatcgt ggcagccgga
     2341 caccgaagac acaaaagact tgaacattcc gtccctggcc gccgtgcgcg cggcatttgt
     2401 cgatgccaaa acggcgatgc gccacatccg ggccaacagc agcacatacg gtatcg
//
LOCUS       DAOPBQ010000541         2453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_33703_length_2453_cov_4.664721, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000541 DAOPBQ010000000
VERSION     DAOPBQ010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33703_length_2453_cov_4.664721"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKW60_13900"
     CDS             complement(<1..637)
                     /locus_tag="PKW60_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPK00574.1"
                     /translation="MKRVTLLSLCQCWLITAAFGAADEAQPTYAELLGWEKGARVLIL
                     HADDAGMSHDSNMGIIEAFEKGVLTSTSTMMPCPWVPEWARYCAEHPDVDNGLHMTLT
                     SEWDDYRWGPVAGKDRVPGLTDEQGCLWDNVGLVVEHASAEELEVEMRAQIDRAKTMG
                     LPITHLDTHMGTVYATPEYFAAYVKIGTEMQIPLMIPGGHATLAQEEHTDPA"
     gene            complement(700..1350)
                     /locus_tag="PKW60_13905"
     CDS             complement(700..1350)
                     /locus_tag="PKW60_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00575.1"
                     /translation="MHQAPPTPTTAYPGSEQNAIAFVPPRMPELQARLWFQKRWYGAM
                     GRVLRLVFPWKRLSRNALPRLELIWMPHYLFTVRAVGPDGDEGRIQFSLDAHAGVFMI
                     VKLAGELDQGECTEQHFPPSMPREEAEILGRKQLTLALLRRRGHRDRPTPHETLDCQL
                     FYYPFWTYYYERMPGAIDIAVLDAVSGEKVGGRTKTAVLDAFTAASARKKSADEAL"
     gene            complement(1445..>2453)
                     /locus_tag="PKW60_13910"
     CDS             complement(1445..>2453)
                     /locus_tag="PKW60_13910"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPK00576.1"
                     /translation="KCFAQNRALGLDPGQGAGPVLREALLTAEGNARIAVINLLGKRR
                     DAEAVPVLCAIANGSDLPAACAAVCALGEIGTGAAANCLANRLEFSEGTVRKAAIEAS
                     LHCAEALVSDGNRDRAMAIYTELAGESYPADVRAAANLGLRNRKLGPPLVLFDGENLE
                     HWEGNLELWRVENGAIVGGSLESPVRHNDFLCTKEHFSDFELRLQVKLSDPEANAGIQ
                     IRSQREPGSPAVRGYQADMGQKYWGCLYDEHRRNEILVNADQAVVRKIIDLSGWNEYT
                     ILCDGGRIQLWMNGHLTADYTEPDDAIPRDGIIGLQIHSGPPSQAYYRNIVLRRIVPL
                     E"
BASE COUNT          419 a          777 c          759 g          498 t
ORIGIN      
        1 cggccgggtc ggtgtgctct tcctgcgcca gggtcgcatg gccgccggga atcatgagag
       61 ggatctgcat ttctgtgccg attttgacgt aggcggcgaa atattccggg gtggcgtaga
      121 ccgtacccat gtgtgtatcg aggtgcgtga tgggtaatcc catggttttg gcacggtcga
      181 tctgggcccg catctcgact tcgagctctt ccgcgctggc atgttcgacg accagaccga
      241 cattgtccca caggcagccc tgttcgtccg tcaggcccgg cacgcgatct ttcccggcca
      301 cgggccccca gcggtagtca tcccattcgg aggtgagggt catgtgcagc ccgttatcca
      361 cgtcggggtg ttccgcgcag tacctcgccc attccggtac ccagggacag ggcatcatgg
      421 tgctggtgga ggtcagaacg cctttttcga aggcctcgat gatgcccatg ttggagtcat
      481 gcgacatgcc cgcatcgtcg gcatgaagaa tgagcactct ggcgcccttc tcccagccca
      541 gcagctcggc ataggtgggc tgggcctcgt ccgcggcccc aaacgccgcg gtgattaacc
      601 agcattggca cagggaaagc agcgtgactc gtttcatgat ttctcgactc tcctttcctc
      661 atcggcgctg gcaaaaattc gccgcggctg cgctccggct caaagcgctt cgtccgcgga
      721 tttttttcgc gcgctcgctg cggtaaacgc gtcaagcacg gcagtcttcg tccggcctcc
      781 gaccttttcc cccgacaccg cgtccaacac ggcgatgtcg atggcgccgg gcatgcgttc
      841 ataatagtac gtccagaacg ggtaataaaa gagttggcaa tcgagcgttt cgtggggggt
      901 gggccggtcg cgatggccgc gccgccggag cagcgccagc gtgagctgtt tgcgcccgag
      961 gatttcggct tcttcgcggg gcatcgacgg tggaaagtgc tgctccgtgc attcgccctg
     1021 gtcgagctct cccgccagtt tgacgatcat gaacacacca gcatgggcat ccagcgaaaa
     1081 ctggatccgg ccttcgtctc catccggtcc cactgcacgg accgtaaaga ggtagtgcgg
     1141 catccagatc agttcgagcc ggggaagcgc gttcctcgac aggcgcttcc agggaaacac
     1201 caggcgaaga acgcggccca tggcgccgta ccaccgtttc tgaaaccata gccgtgcctg
     1261 gagctcgggc atgcgcggag ggacaaaagc tatggcattc tgctccgaac cgggatacgc
     1321 agtggtaggg gtgggcggcg cctgatgcat gtttccgttc ttcgtcctct cctctggtct
     1381 ggggccgcgc ggcttgcgtg gtccttttct ccaacctggc gtccggaagg ccggagcctg
     1441 tccgtcattc aagcggcaca atccgccgga ggacgatatt ccggtaatac gcctggctgg
     1501 gcggtccgct gtgaatctgc aggccgatga tgccatcccg ggggatggca tcatcgggtt
     1561 cggtatagtc ggccgtcagg tggccgttca tccagagctg gatgcggccg ccgtcgcaca
     1621 ggatcgtgta ctcgttccag ccgctcagat caatgatctt ccggaccacc gcctgatccg
     1681 cattgacgag gatctcgttg cggcgatgtt catcgtagag gcagccccag tatttctggc
     1741 ccatatccgc ctggtacccg cgcacggcag gactacccgg ctcgcgctgg ctgcggatct
     1801 ggattcccgc attggcctca ggatcggaca gcttgacctg cagccggagc tcaaagtcgc
     1861 taaaatgttc ttttgtgcac aggaaatcgt tgtgccgcac cgggctttcg agcgaaccgc
     1921 cgacgatcgc gccattctcg acgcgccaga gttccagatt gccttcccag tgttccagat
     1981 tctctccgtc gaagagcacc aggggcggac cgagtttacg gttgcgcagg cccaggttcg
     2041 cagccgcgcg cacgtcagcc ggataggact ctcccgcgag ttccgtgtag atggccatgg
     2101 cccggtcccg gtttccgtcc gaaaccagcg cttccgcgca gtgaagcgag gcctcgatcg
     2161 ccgctttgcg gaccgtgcct tccgaaaact ccagccggtt ggcgaggcaa ttggccgcag
     2221 ccccggtccc aatctctccg agcgcgcaaa cggctgcaca ggcggcgggc agatcgctcc
     2281 catttgcgat cgcacaaagt acgggaaccg cttcggcatc ccggcgcttg cccaacaggt
     2341 tgatcacggc aatgcgggca ttgccctccg cggtgagaag tgcttcgcgc aaaaccgggc
     2401 ccgctccctg cccagggtcc aggccgaggg cccggttttg cgcgaagcac ttc
//
LOCUS       DAOPBQ010000542         2450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_33763_length_2450_cov_3.539875, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000542 DAOPBQ010000000
VERSION     DAOPBQ010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33763_length_2450_cov_3.539875"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="PKW60_13915"
     CDS             <1..1261
                     /locus_tag="PKW60_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00577.1"
                     /translation="GNRLVDDDEFARVMSSTTGSITGGVAYGVPHGSTYNLGDLPSAS
                     NGAANSLASNGTYSGNLVLVGTEANPIEIHGKVAIDGDLVIQGPVKGWGQLLVRGNTY
                     VTGDVTYKDAPGEFGVASDGTENGLALTSGGSVLMGDYLTIRGKNHTADTSKFPDTSL
                     SIRMREARKTKSVTKKVGNKNVTQTMEYGYFDPGVVDAGNIQTTMVAGGKQVTRTGQQ
                     FSFTTSELMLFNNLELEKAIADPSYKPRFYGLRESQPTDIYVYDSPDEHSVRYDESGG
                     GVRKLSDYLIDKRLPLSILNNATIHYMNPAGNWISEDTLRQIWWNDEQTRPPSGRPFQ
                     FDGLVYSNNAVFTLTRSFVRHGSYAYGAMRIRGGMVAADLGVLIPGEGNTVGLTLEYD
                     RRVRRFLNVEDTTKVTFSRLVYLQDQG"
     gene            1273..2205
                     /locus_tag="PKW60_13920"
     CDS             1273..2205
                     /locus_tag="PKW60_13920"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPK00578.1"
                     /translation="MIRNFMPAALFGAFSMILCVCALCVPSAGADTVTMNNGTRIDGQ
                     VQEKPGGRMVVIVDGRSIELRPEEVAQVEKNEKTGGLDQAALEARRAERDRRHLEETG
                     LTLEQRMEVMELMRNLQSSDSKLKERSREALLAKGKELDLFQFFELYVRGLSPRFIPA
                     VLEVLCTFDAPRAIPLVREHCLHVDANTRITAITLLSKEQDYNSVELIARGLLDHEPD
                     VQIAASNALAALRARETTPILLDNLQSADARVRYVSRHALARIWSEPDAPVDFETHEE
                     WAAFWASEAKAVDNPITTAGLEPLVKPGERFEDE"
BASE COUNT          563 a          709 c          715 g          463 t
ORIGIN      
        1 cgggaaccgg ctggtggacg acgatgaatt cgccagggtc atgagcagca caacagggtc
       61 gatcaccggc ggcgttgcgt atggcgtccc ccatggttcc acatacaacc tgggggattt
      121 gccttcggcg tcaaacggag ccgccaattc acttgcatcc aacggcacct acagcggcaa
      181 tctggtgctt gtgggtacgg aagcgaatcc catcgagatc cacggaaaag tcgctattga
      241 tggcgatttg gtgattcagg ggccggtcaa gggatggggg caactgctgg tacgcggcaa
      301 cacctatgtc accggagacg tgacctataa agatgcgccc ggggaattcg gtgtggcgag
      361 tgacgggacg gagaacggcc tggcgctgac gtcgggcggc agcgttctca tgggtgatta
      421 tctgaccatt cggggaaaaa atcacaccgc ggacacgagc aagttccccg atacttccct
      481 cagcatccgg atgcgggagg ctcgaaaaac aaaaagcgtc acaaaaaagg tcgggaacaa
      541 aaatgtcacg cagacaatgg aatacggtta tttcgatccc ggcgtggtgg atgccggcaa
      601 catccagaca accatggtgg ccggcggaaa gcaagtgacg cgcaccggtc agcaattcag
      661 ctttactacg tctgaattga tgcttttcaa caatctggaa ctcgagaaag ccatagcaga
      721 tcccagttat aagccgcggt tctacgggct gcgggaaagc cagcccacgg atatctatgt
      781 ctacgactct ccggatgaac actccgtgcg gtatgatgaa tcgggcggcg gcgtccggaa
      841 gctgtcggac tatctgatag acaaacggct ccccctcagc atactcaaca acgccacgat
      901 tcactatatg aatcctgcgg gcaactggat ctccgaagac accctgcggc agatctggtg
      961 gaatgatgaa cagacgcggc cgccgtccgg gcgcccgttt caatttgacg ggctcgtcta
     1021 cagcaacaac gcggtcttca cgctgacccg cagctttgtc cgccacggct cgtatgccta
     1081 cggcgcgatg cggatccgcg gcggcatggt ggcggccgac ctgggcgtgc tcattcccgg
     1141 tgaagggaac acggtcgggc tgacgctgga atacgatcga cgcgtgcgcc gcttcctgaa
     1201 tgtcgaagat accaccaagg tgactttttc tcgtctggtc tacctgcagg accaaggtta
     1261 ggagtacagc tcgtgattcg caatttcatg ccggccgctc tcttcggggc attttcgatg
     1321 atcctgtgcg tctgtgcgct ctgcgtacca tccgccggcg ctgataccgt taccatgaat
     1381 aacggtacac gaattgatgg acaggtgcag gagaagcccg gggggagaat ggttgtgatc
     1441 gtcgatggac gatccatcga gctgcggccg gaggaagtgg cccaggtaga aaaaaatgaa
     1501 aagacaggcg gcctggatca ggccgctctg gaggcgcgcc gggcggaaag agatcggaga
     1561 cacctcgaag agaccgggct gactctggag cagcgcatgg aagtgatgga actgatgcgc
     1621 aacctccaat cctcagattc gaaactgaaa gagcgttccc gggaagcctt gctcgccaaa
     1681 ggaaaggaac tcgacctctt ccaattcttc gagctctacg tgcgaggcct gtcgccccgc
     1741 tttattcccg ctgtgttgga agtcctgtgc acgttcgatg cccctcgcgc cattccgtta
     1801 gtgcgagaac actgcctcca tgtggatgca aacacacgca tcacggcgat aaccctgcta
     1861 agcaaagagc aggactacaa ttccgtcgag ctgattgcgc gcggcctcct cgaccacgaa
     1921 cccgacgtgc aaattgcggc atccaacgca cttgcggcac tgcgcgcgcg ggaaaccacc
     1981 ccgattctcc tggataacct gcaatctgcc gatgcccggg tgcgttatgt cagccggcac
     2041 gccctcgccc gcatttggag cgagcccgat gcccccgtgg acttcgagac gcacgaagaa
     2101 tgggccgcct tctgggccag cgaggcaaag gctgtggaca accccatcac aacagccgga
     2161 ctcgagcccc tggttaagcc gggagaacgt ttcgaagacg aatagcggct tgcccgcatc
     2221 gccaccgccg ctctaccgca cgtcatctat tggaacagac gtggagtctc cgcgcgattc
     2281 ttgcagagca aagcctgcct cgcgccggat ctgaattctc cgcattgttc gccacaaaag
     2341 cgaaatctcc gaatcagtaa tatgcagagg cacagccttt cctccaccta atggacgcgc
     2401 gagacccaga tgaatctgga aaagcggctg agtctgtgct acactctccg
//
LOCUS       DAOPBQ010000543         2445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_33848_length_2445_cov_4.465272, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000543 DAOPBQ010000000
VERSION     DAOPBQ010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33848_length_2445_cov_4.465272"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1792
                     /locus_tag="PKW60_13925"
     CDS             <1..1792
                     /locus_tag="PKW60_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HPK00579.1"
                     /translation="FQAEAFQALTGYYTKLFQDGNDRMGLQPGIVRTEEEGRLVTDFF
                     AWLSWAAGTRRPGSEYTYTTNWPYDPLVGNKPLPDFLIWSIVSVILLIFAIAAALFAY
                     QRYVGQSEYGSHLSLDFPEPEPTSSQKATLAYFITAIVLFILQLGLGAVTAHYTVEGT
                     SFYGIPLGSFLPYAAVRTWHIQLAIFFIATCFLAAGLFIGPFVGREPKGQAAWVMVLF
                     AALVVVVAGTLGGTWLSVSGFFGADGYLFGHQGYEFIELGRVWQLLLIAGMIIWLVLV
                     WRAIRPALKDEKDSGGLTHMLLYSSVSIPLFYMAGLMYGKGSHLSDAEYWRWWVVHLW
                     VEGFFEVFATVVMAFLLSHIGAVSKKFALTSVYFIVFLYLGSGVIGTFHHLYWSGTPT
                     MIIALGAVFSALEVVPLSLLGFEAAHNLSVVDEGGKTYAYKWPIYFFVSVAFWNLVGA
                     GVFGFLINPPIVLYYSQGINTTPIHSHGALFGVYGMLAISLMLFSVRHIVTRASWSDK
                     LLKYSFWGLNGGLAGMMVLSLIPSGFYQFYHAIREGLWYARSPEIASGQFIRTVTWFR
                     MLPDILFAVGALALLAFLVRATMLSFRKKA"
     gene            complement(1848..2231)
                     /locus_tag="PKW60_13930"
     CDS             complement(1848..2231)
                     /locus_tag="PKW60_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPK00580.1"
                     /translation="MGKFGVTPQKEAELAQRMDACGLRESDLEERFVSSSGPGGQHVN
                     RSATCVQLRHRPTGLEVKVQTARSQALNRFYARRRLCELIEARELGKESPEAKRAARA
                     RKQKARHRRKAARKQADRAGSDTPD"
BASE COUNT          376 a          751 c          748 g          570 t
ORIGIN      
        1 gttccaggcc gaggcgttcc aggccttgac gggctactac acgaagcttt tccaggacgg
       61 caacgaccgc atgggcctcc agccgggtat tgtgcggacc gaagaagaag gccgcctggt
      121 gacggacttt tttgcatggc tttcctgggc tgctggcacg cggcgccccg gttcggaata
      181 tacctacaca acgaactggc cctatgatcc cctggttggg aacaaaccgt tgcccgactt
      241 tctcatctgg tccattgtga gcgtcattct gctgatcttc gccatcgccg cagccctgtt
      301 tgcctatcaa cggtacgtag ggcagtcaga gtacggaagc catctgtccc ttgatttccc
      361 ggaaccggaa ccgacatcga gccagaaggc caccctggcg tacttcatta cggcgatcgt
      421 gctgttcatc ctccagctcg gcctcggtgc ggtgacggcg cactataccg ttgaaggcac
      481 ttccttctac ggtatcccgc ttgggtcatt cctgccgtat gcggccgtca ggacgtggca
      541 cattcagctg gccattttct tcatcgcgac ctgcttcctg gccgccgggc tcttcatcgg
      601 cccgttcgtc gggcgggagc cgaaaggcca ggccgcgtgg gtgatggtcc tttttgcggc
      661 gctcgtcgtt gtcgtcgcgg gcaccctggg cgggacctgg ctttccgtgt ccggtttctt
      721 tggcgcggac ggctacctgt tcggccacca gggttacgag ttcatcgagc ttggccgggt
      781 atggcagcta ctgctgatcg cggggatgat catctggctg gttctggtct ggcgagcgat
      841 ccggccggcc ctcaaggacg aaaaagacag cggcgggctc acgcacatgc ttctctacag
      901 ctcggtatcc attccgctgt tttacatggc cgggttgatg tacggaaagg gcagccacct
      961 ctccgatgcc gaatactggc ggtggtgggt ggttcatctg tgggtcgaag gcttctttga
     1021 ggtatttgct acggtcgtca tggcgtttct gctgtcacat atcggcgccg tgagcaaaaa
     1081 gttcgccctg accagcgtct atttcatcgt atttctctat ctgggaagcg gggtgatcgg
     1141 caccttccac cacctgtact ggagcggaac cccgacgatg attatcgccc tgggcgcggt
     1201 tttctccgca ttggaagtgg tccctctgtc gctgctgggg tttgaagcgg cgcacaatct
     1261 gagtgtcgtg gacgaaggcg ggaaaaccta tgcctacaaa tggcccatct acttctttgt
     1321 cagcgtggcc ttctggaatc tggtcggggc gggcgtattt ggatttctga tcaatccgcc
     1381 gatcgttctg tactattcgc agggcatcaa caccaccccg attcactcgc acggagcgct
     1441 gttcggcgtc tacggcatgc tcgccatctc tctgatgctc ttctcggtcc gccacatcgt
     1501 gacacgggcc tcgtggtcgg acaagttgtt gaaatacagc ttctggggcc tcaacggcgg
     1561 gctggcgggc atgatggtgc tgagcctgat tccttcggga ttctatcagt tctaccacgc
     1621 catccgggaa gggctgtggt acgcccgcag tccggagatt gcctcgggcc agttcatccg
     1681 caccgtcacg tggttccgca tgctccccga catccttttt gccgtagggg cgctggcgct
     1741 gcttgccttc ctggttcggg ccacgatgtt gagcttccgg aaaaaagctt gaccgtttac
     1801 cggcaacgac tgaaaaacgc gctacggagc gcagtgcgcg gaccgcgtca atcgggtgtg
     1861 tctgacccgg cgcggtccgc ttgttttctg gctgctttgc ggcggtggcg ggccttttgt
     1921 ttgcgcgccc tggccgcgcg cttcgcctcc gggctctcct tccccagctc tcgggcttcg
     1981 atgagctcac agagccggcg ccgggcataa aaccggttca gcgcctggga acgtgctgtc
     2041 tgcaccttga cttcgaggcc cgtgggtcga tgccggagct ggacgcaggt ggccgaccgg
     2101 ttcacgtgct ggccgccggg gccgctggac gagacgaagc gttcttcgag gtcggattcg
     2161 cgcaggccgc acgcgtccat gcgctgcgcg agctccgcct ctttctgggg cgttacgcca
     2221 aacttgccca tcgcatttcc cctgcggccc gggtcacccg tccgggcgat ttaattcgag
     2281 ccggcgtgca tttgcagggc cgcctcaatc tcgccgaaca gggcgcgcgt cgcctcgttg
     2341 acctggccgg cctggtcgcg ctgaaagtag cgggacacgc catcgcccgt catttgcacc
     2401 cagacatgct cgccctgccg gtacggttct ttcagatgaa gcgtc
//
LOCUS       DAOPBQ010000544         2444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_33867_length_2444_cov_4.312265, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000544 DAOPBQ010000000
VERSION     DAOPBQ010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33867_length_2444_cov_4.312265"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..1938
                     /locus_tag="PKW60_13935"
     CDS             334..1938
                     /locus_tag="PKW60_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPK00581.1"
                     /translation="MIRFFQSITVLALALAAGGAAAEPPRMALGAGPRTGDVYVVEVP
                     VSSADAAAELARREFNIASIRENRAEIYAVREELELLDALGYSWHVIRRDGDGTKALG
                     EYNGYASVTAMLAAYAQAYPAITRLESVGQSVEGRELWVLYISDNPGIEEDEPEFKYI
                     GTIHGDEPLGTEMCLHFIDLLLTGYGQIPRITDLVDNTSIAILPLLNPDGREAGTRMN
                     ANGVDLNRAFPRYPFDYTGNQGDGEPLQAAGRQPEVVRVMEWTAANRFVLSANFHTGA
                     LLVNYPYDEDGVPSGADAPTPDDALFQELARRYARENLPMWNSPYFADGISNGSAWYS
                     IFGSMQDWNYRYMACNEVTIELSNTKWPPAGALPAFWDDNREAMLRYLEAVHLGVRGL
                     VTDALTGQPLDARVTVQGNAQPVFTDPDVGDYHRTLLPGVYTITIRANGYYPQTFINL
                     IVTSGPATRIDAALIPLDAPFDADVNNDGEVNAIDVQLVINAALGLSVPAGADINADG
                     AVDAIDIQLVINAALTRSYAPATAQE"
     gene            2174..>2444
                     /locus_tag="PKW60_13940"
     CDS             2174..>2444
                     /locus_tag="PKW60_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00582.1"
                     /translation="MSTCAYCEAAAAVRRGAGAAWDVYLFACPECLNVSLLRNRSNGI
                     WAERLETEPDIREVLPPGSVPAEILGRIRKAADDLPLLPEVCQRVV"
BASE COUNT          448 a          734 c          799 g          463 t
ORIGIN      
        1 ggagtttttc taatggatgg gcaggcccct gtgcccgccc atcgggatta tatggacaac
       61 ctcgacgcga tcgcgttccg gaatgtaggg gcagacacag gggtctgccc ctacgcgctg
      121 cggaaatcag tccccgcgtg gatgcccttc cgcatagccc ctgcgggtgc gttgaagaag
      181 gttgggcttc cgggatcgcg ctgacattcc gggcgccgta tcgcggcagg agtcgagggc
      241 gccggggctg gtatactgcg caagtgtgca tcatgtggat gaactggtgc gctggtgagt
      301 gttgatcggg gcgggaagcc cgtttggagt gcagtgattc gttttttcca gagcataacg
      361 gtgttggccc tggcgctggc cgccggcgga gcagcggcgg aaccgccccg gatggcgctg
      421 ggggccggtc cgcgtaccgg ggacgtgtat gtggtcgagg tgccggtttc cagcgcagac
      481 gcggcggcag aattggcccg gcgggagttc aacatcgcct caattcggga gaaccgggcc
      541 gagatctatg ccgtccgcga agagctcgag ctgctcgatg cgctgggtta ttcctggcac
      601 gtgatcaggc gcgatggcga tggcacgaag gcgctgggcg aatacaacgg gtacgcgtcc
      661 gtcacggcca tgctcgcggc ctatgcgcag gcctatccgg ccatcacgcg gctcgagagc
      721 gtgggccagt ccgtggaggg ccgggaactc tgggtcttgt acatcagcga caaccccggc
      781 attgaggaag atgagcccga attcaagtat atcggcacga tccacggcga tgaaccgctc
      841 ggcaccgaga tgtgcctgca tttcatcgat ctgctgctca ccggatacgg gcagatcccg
      901 cgcatcaccg acctcgtcga caacacgtcg atcgcgatcc tgcctttgtt gaatccggac
      961 ggacgcgaag ccggtacgcg gatgaacgcc aatggtgtgg acctcaaccg ggcgtttcca
     1021 cggtatccgt tcgattacac cggcaatcag ggcgacggag aaccgctcca ggccgccgga
     1081 cggcagccgg aagtggtgcg cgtcatggaa tggaccgccg cgaaccggtt tgtgctgtcc
     1141 gcgaactttc acacgggcgc gctgctcgtc aattacccgt acgacgagga cggcgttcca
     1201 tcgggagcgg atgcccccac gcccgatgat gcgttgtttc aagagctggc ccggcgatac
     1261 gcgcgcgaga atctccccat gtggaacagc ccgtactttg ccgatggaat ctccaacggt
     1321 tcggcctggt actccatttt cggcagcatg caggactgga attaccggta catggcctgc
     1381 aacgaggtga cgatcgagct ttcgaacacc aaatggcccc cggccggcgc gttgcccgca
     1441 ttctgggatg acaaccggga ggccatgctg cgctatctcg aagccgtgca tctgggcgtt
     1501 cgcggcctcg tcacggatgc gctcaccggc cagccgctgg acgcccgggt gacggtccag
     1561 ggcaacgccc agcccgtttt taccgacccg gatgtgggcg actaccaccg cacgcttctg
     1621 cccggcgtct ataccattac catccgggcc aatggctatt atcctcagac cttcatcaat
     1681 ctgatcgtga cttccgggcc ggccacccgc atcgatgccg ctctcatccc gctggatgcg
     1741 ccgttcgatg ccgacgtcaa caacgatggc gaggtcaacg cgatcgacgt ccagcttgtc
     1801 attaatgcag ccctgggcct ctctgttccg gccggcgccg acatcaacgc agacggagcc
     1861 gttgacgcca tcgacatcca gctcgttatc aacgccgccc tgacccgcag ctacgccccg
     1921 gctaccgctc aggaatgagt gtttcggcgc ctgcacggca tgcccggcgg gaaagggcgc
     1981 tgcatcggtt ctgactgcca aaacaactgt gctaaaacgc ataaattccg ggatttatct
     2041 gcgtctacac tggaattgat gggtctttac tggtaaaata gtggagacaa gtaaggacgc
     2101 attgcgcgag cataagcgaa cggaatcgtc gcggtccgca tggggggatg catcggcaga
     2161 cgggaggacg gccatgagca catgcgcgta ttgcgaggcg gcggctgcgg tccgacgggg
     2221 agcgggggct gcgtgggacg tttatctgtt cgcttgcccg gaatgtctga acgtctcatt
     2281 gctgcggaac cgctcgaacg ggatatgggc ggagcgcctg gagacggagc cggatatccg
     2341 cgaagtcctg cctcccggtt cggttcccgc ggaaatcctg gggcgtatac gcaaggctgc
     2401 cgatgatctg cccttgctgc cggaagtgtg ccagcgcgtg gtgg
//
LOCUS       DAOPBQ010000545         2439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_33952_length_2439_cov_4.251258, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000545 DAOPBQ010000000
VERSION     DAOPBQ010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33952_length_2439_cov_4.251258"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PKW60_13945"
     CDS             <1..903
                     /locus_tag="PKW60_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00583.1"
                     /translation="NDICWLNVVVAGMAPSAEIPRFLRGMRNGYLLNSPAGTALIDAY
                     YRTAPPLARYLAAHPVALGYARSVFSGLEWVFRHAEMLLMATAGLLGLRVLLELRRWR
                     AARTAAGLLVAAGLLLACGSAHALLARMTPEEMVKASDYIFVGTVEAAESYYLKENVI
                     VTDVTIRTDDNVKGHLNKGGQIVLRQPGGRVGGIMRYVTDLPQFHEGEEVVVFMQAEK
                     QRGPAGVVCGERGRLPVRTDALNGEKYVVFGETALGVSQTEKADAPENASAPKRMELE
                     EFKAYLRGIDQKLRADEEAAENAE"
     gene            1042..1230
                     /locus_tag="PKW60_13950"
     CDS             1042..1230
                     /locus_tag="PKW60_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00584.1"
                     /translation="MRGWAGRPLQAGFSLIYAQFRPRSRAIVVFDPIPGLVYDWQPDA
                     GDAIAGHVNLLMAGFVVW"
     gene            1332..2231
                     /locus_tag="PKW60_13955"
     CDS             1332..2231
                     /locus_tag="PKW60_13955"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HPK00585.1"
                     /translation="MLPGLLIRKVFLLLDLVLIVLLLAVAYTAFSTFFEPVRAVETPP
                     PPEAVEGLHLAKVGLPADYSVIKENGLFGPAAESSAPDPAAAVVAAPTVEVTRLPLKL
                     LGTAATSPTDPLACAGIKNTQTNSENTYYLGEPVLSDVVLEEVHKRGVVLFNKKENKR
                     EILSMDEKETIPAHLTAVSGAAAAPQPADGGEPGPVSIKKDELMEIASDMGRLMMELK
                     PQPYKDESGKMIGITSPDLGKVPLAKKVGLADNDVITAINGMSIDSVESIGKIAEKFK
                     NLNVFHLKVLRNGQTVTRTIKLD"
BASE COUNT          493 a          646 c          826 g          474 t
ORIGIN      
        1 aatgacatct gctggctgaa cgtggtggtg gcgggcatgg cgccatccgc cgagattccc
       61 agattcctgc gcgggatgcg caacggctat ttgctgaatt cgccggcagg tacggcgctc
      121 atcgacgcct attaccgcac ggcgcctccg ctggcccggt acctggcggc gcatccggtg
      181 gctctgggct atgcgcgcag tgtgttcagt ggactggaat gggtgttcag gcatgcggag
      241 atgctgctta tggcgacggc cggattgctg ggattgcgcg tactgctgga actgcggcgc
      301 tggcgcgccg cccgaaccgc tgcgggcctg ctcgttgcag ccggcttact ccttgcatgc
      361 ggctcggccc acgccctgct ggccaggatg acgcccgagg agatggtgaa ggcgtcggat
      421 tacatcttcg tgggtacggt ggaagctgcc gaaagctact acctgaagga aaacgtaatc
      481 gtgacggatg tgacgatccg aacggacgac aacgtgaagg gccatctgaa taagggtggt
      541 cagattgtgt tgcgtcagcc gggcgggcgc gtaggcggaa ttatgcgcta cgtgaccgat
      601 ctgccgcagt tccacgaagg cgaagaagtg gtcgtgttca tgcaggccga gaaacagcgc
      661 ggtcctgccg gcgtggtctg cggagaacgg ggcagacttc cggttcgcac cgatgcgctg
      721 aatggcgaga aatacgtggt gtttggggag accgctctgg gtgtttctca gacagaaaaa
      781 gcagacgcgc cggagaacgc gtccgctccc aagcgtatgg aactggaaga gttcaaggcc
      841 taccttcgcg gaatcgatca gaaactcagg gccgatgaag aggctgcgga aaacgccgaa
      901 taacggcgca gtgtaagagt ataccccccc ggagactggc gtctccaggg ggggttttgt
      961 attcccgggg aggtcggagt gcagagtggc gggcgccagc gttccttgca ttggcggtat
     1021 gtggattgcg ccggacagcg aatgcggggg tgggccggca ggcctttgca agccggtttc
     1081 agcctgattt atgcgcaatt ccggcccagg tcgcgcgcca tcgtggtctt tgaccccata
     1141 ccgggcttgg tatatgattg gcagccggac gcgggagacg ccatcgcggg gcacgtcaac
     1201 ctcctgatgg cggggttcgt tgtatggtag aagcatgcgg gcaggggtgt ttcgtgcctc
     1261 gctgacgaga ttttcccggt taggagggtt gagccccgcg tcctgctctt ccggactcag
     1321 gaaaggcaaa aatgctccca ggattgctga tacgcaaagt ttttctactg ctggacttag
     1381 tgcttattgt gctccttctg gccgtggcct atacggcctt ttcgaccttt tttgaaccgg
     1441 ttcgggccgt tgaaacgcca ccgcctccgg aagcggtgga gggcctgcac ctggccaaag
     1501 tgggactgcc ggcggattac agtgtgatca aggaaaatgg cctctttggc cccgcggcag
     1561 aatcgagcgc tccggatccg gccgcggccg tagtcgcggc gccgaccgtg gaggtaaccc
     1621 ggctcccctt gaaactgctg ggtacggcgg cgacctcgcc caccgaccct ctggcgtgtg
     1681 ccggcatcaa aaacacgcaa acgaacagcg aaaacaccta ttacctgggc gagcccgtcc
     1741 tttcggatgt ggtgctcgaa gaagtccaca agcgcggggt cgttctgttc aataagaaag
     1801 aaaacaagcg cgagatcctg tccatggacg aaaaggagac gattcccgcg catctgacgg
     1861 ccgtttccgg agcggccgcg gcgccgcaac ctgcggatgg cggagagcct gggccggtgt
     1921 cgatcaagaa ggacgagctc atggagatcg ccagcgacat gggccgcctg atgatggagc
     1981 tcaaaccgca gccatacaag gacgagagtg gaaaaatgat cggcatcacg tcgccggatc
     2041 tgggaaaggt gccgttagcg aaaaaggttg ggctggcgga caatgacgtg atcacagcca
     2101 tcaacggcat gtcgattgac agtgtcgaga gcatcggcaa gatcgcagaa aagtttaaaa
     2161 acctcaacgt gttccatctg aaggtgctgc gaaatggcca gaccgttacg cgcaccataa
     2221 agttggacta ggggtgcgcg ggtgccgtcg aactgcggcg cgcctgcggg ttgcatgcgt
     2281 tttagtgtct tgaagctatt gaaccagggg aaggaaaaac cgtgaactac ggctggataa
     2341 ccacgcgggc gttggtatgc cttgggatcg cgctgatgat actggttttg ccggcgcgtg
     2401 cgcagcagga tgctgtggat gatgcggcgc tggcggaag
//
LOCUS       DAOPBQ010000546         2392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_34839_length_2392_cov_3.507916, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000546 DAOPBQ010000000
VERSION     DAOPBQ010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34839_length_2392_cov_3.507916"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(383..1402)
                     /locus_tag="PKW60_13960"
     CDS             complement(383..1402)
                     /locus_tag="PKW60_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK00586.1"
                     /translation="MTTVRQIAKLANVSICTVSKALRDDPRISAATRERVQAIAQSLY
                     YRPNRLATGLYTGTTSLIGMILPELNTWYGVQILRVLTQECYSAGWNMLVFEHLWHPS
                     LLDELIVQQVERRVQGIILIPGVLPFPKRYLAELGARRIIPVGIDLEQQEHAVDQVRI
                     DEEALAHLAIEYLLHLGHRRIGFVSIEKSKRTGRDLAIRRELARRGLADSRYQFHHSG
                     TQLPDVRRAYSDLLGRSPRPTAVIAWSFEMALSFMQVAAELGISVPQDMSVFSCDNEA
                     YADKTTPPLTCLDVHPEEVGRQAFHLILRRLKAYADTPETTPLTPEIISVKPTLIKRA
                     SCLRI"
     gene            1628..>2392
                     /locus_tag="PKW60_13965"
     CDS             1628..>2392
                     /locus_tag="PKW60_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00587.1"
                     /translation="MSTEHTLSNTAPPWHGVFTANGDGQFLYQSDAYRVAFDRTGRLL
                     SLRVNGHEFLSVDGTELAGGCFVADGLPVGLPHINLQADTAALVNGHAGIFLQATPDR
                     LQLDLGHENPLGYQDLVVFPADDVVITPLEHAVMREQRRLPAYTRFQCAESAMRWTAP
                     DGTAIEWQYAAAIVPDYHGRRAMIISVPFGSRLCGEIRFPASVWAADAVDITLTVGAP
                     DHCFAAGAPIAVQGTVARKAETATVIELTLRISDLNT"
BASE COUNT          478 a          689 c          735 g          490 t
ORIGIN      
        1 ccgagcccgg tgttgaacgt taataaatac acatttgcag caattattgt tgtgcaatgc
       61 gtgcgtcatt cggactgtgt cggcgagcac ctgtcggcca tcgaagcacc tcaccgcata
      121 ccattcacga tcgatggatg agctgcaccg tgtctccctg ctacggcgcg aaacgcgaaa
      181 agcgtctgtt gctccgccca gggcagtgga aggcgtgggg gccatgtgcc cttgaagaat
      241 ggcgtccacg tgatccgacg cgagtaaggc gtgggtcccg ccacagcggc atttttccgc
      301 cgtgtctggc gaaaaatccc cgcaaaatct gggcatcgcc gatagggtga acacgccctg
      361 gcttagatta aacgtccagg gattaaatgc gtagacacga tgcgcgttta atgagcgtgg
      421 gtttcacaga gatgatttca ggcgtgagcg gcgtagtctc cggcgtgtcg gcgtaggctt
      481 ttaggcggcg cagaatcagg tgaaaggctt gccggcccac ctcttcggga tgtacatcga
      541 gacaagtgag gggcggagtc gtcttgtcgg cgtaggcttc gttgtcacaa ctgaatacgc
      601 tcatgtcctg gggcacgctg atgccgagtt ccgccgcgac ctgcatgaac gagagcgcca
      661 tctcaaagct ccaggcgatg accgctgttg ggcggggaga gcgtccgagg agatcgctat
      721 acgcccgccg cacgtccggc agttgcgtgc cggaatgatg gaactggtat cgggaatccg
      781 ccaggccacg gcgcgccaac tcacggcgga tcgccaggtc gcggcccgtc cgcttgcttt
      841 tttcgatgga aacgaacccg atccggcgat ggcctaaatg cagcaggtat tcgatggcaa
      901 gatgggccag cgcctcctcg tcaatgcgca cctggtcgac cgcatgttct tgttgctcca
      961 gatcgatccc cacgggtatg atccgtcgcg cgcccagctc tgccaggtag cgcttgggga
     1021 agggcaacac gcccggtatc aggataatcc cctgcacacg ccgttccact tgctggacaa
     1081 tcaattcatc cagcaggcta gggtgccata agtgctcgaa gacgagcata ttccaccctg
     1141 cggagtagca ctcctgggtg agcacccgga gaatttggac cccataccag gtgttgagct
     1201 ctggaagaat catcccgatc agggatgtcg tacctgtgta cagcccggtc gccaggcgat
     1261 tcgggcgata gtagagtgat tgcgcgatcg cctgcacgcg ctcgcgcgtc gccgcactga
     1321 tgcgcgggtc gtcgcgcaac gccttggaga cggtgcagat cgagacgttg gcgagcttgg
     1381 cgatctgacg aacagtggtc atacaagacc tcttgagata acttgttatc tgcagaatac
     1441 cacacaaagc ccttgctgac aagcgagatg acgcctatca tcattttgtc cgatatgcag
     1501 ccggcgaaga ctgttcacat ggcacacgaa gcatttccag tggtctccac gaagaaccgg
     1561 ctctgtgccg ctctcgccgt tgggcatgag cgtagaaaca gagacgggaa cggtgagaaa
     1621 gggactgatg tctaccgagc atacactatc caacacggca cccccctggc acggcgtttt
     1681 caccgccaat ggtgacggcc agtttctcta ccaatccgat gcgtatcgag tggcatttga
     1741 ccgcacaggc cgtctgctgt ccttgcgggt gaacggacac gaatttctct cggtagatgg
     1801 gacagaactg gccggcggct gttttgtcgc ggatgggctg cctgttggtt taccccatat
     1861 caacttgcag gcggatacgg cggcattggt gaatggacat gccggtatct ttctccaggc
     1921 gacaccggat cgcctgcaac tggacttggg ccacgaaaat ccgctcgggt accaggacct
     1981 ggtggtcttt cccgccgatg atgtcgtcat caccccgctg gaacacgcgg tgatgcggga
     2041 gcaacggcgg ctgcctgcat acacccgttt tcagtgcgcc gagtccgcca tgcgctggac
     2101 ggcgccggat ggcacggcca ttgaatggca atatgcggcg gcgatcgtgc ctgattacca
     2161 tgggcggcgt gccatgatca tttcggtgcc gttcggcagt cgcttgtgtg gtgaaattcg
     2221 tttccccgcc agcgtctggg cagcggatgc cgtggacatc accctgaccg tcggcgctcc
     2281 ggatcattgt tttgccgccg gcgcgccgat cgccgtccag gggaccgtcg cgcggaaggc
     2341 cgaaaccgcg acggtgatcg agctgacgct gcggatcagc gatctgaaca cg
//
LOCUS       DAOPBQ010000547         2391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_34869_length_2391_cov_3.437500, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000547 DAOPBQ010000000
VERSION     DAOPBQ010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34869_length_2391_cov_3.437500"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PKW60_13970"
     CDS             complement(<1..946)
                     /locus_tag="PKW60_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPK00588.1"
                     /translation="MELPPLAKELSRKIAAKACVGGVIGLGYVGLPLARHLCGAGYRV
                     LGFDVDPEKTERINRGESYIKHIDSGWIGAAVTNGSFSATTAFKRLAETDCISICVPT
                     PLDEHLEPNLQYVRATAESIAKTLRPGQLVLLESTTYPGTTTEILLRLFENTGLKAGE
                     DFFLAYSPEREDPANPEFSISNIPKVLGGVTPACAELAAQYYATIFKRIVRASSPEVA
                     ELSKLLENIFRSVNIALVNELKLLCTRMGLDVWEIIDAAATKPFGFMPFYPGPGLGGH
                     CIPIDPFYLSWKAREYGMATRFIELAGQITTLMPEYVVH"
     gene            1218..>2391
                     /locus_tag="PKW60_13975"
     CDS             1218..>2391
                     /locus_tag="PKW60_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00589.1"
                     /translation="MKRVSIVLGVGILLGLCARGAELPAPPAPLTGQALPGMPFTTSE
                     DARAAFKAVGNTAPVDVVEAGGRAMLRMPCVFEGVETERAVWDWRGQLDLTRSQGLQF
                     YFYSPDTSPVSYFSLYLRSGDGWYHMTFGAKAPGQWTPVVFDKSATDLEGRPQGWGSI
                     DLVRIAAWRGKHQNTEFYVADLSLYGADAPIGIVRNESAAADKNSEYKIAAQCTGFVA
                     EQLGALGIPFIIMSDLDLSNAFLRDKQLIILPYNPSMSDAAVQALARFLERGGKLMSF
                     YRLDSRLAPLMGVKSGRYVGRDQNVTFGSIRAAGTPLEGQPAEVTQHSWNIQDVLPVD
                     GKSRAVAAWYDEAGLPTGCNAIVASANAVHMSHILLTEDPVRKRMLLLAMTGHLLPD"
BASE COUNT          417 a          721 c          799 g          454 t
ORIGIN      
        1 ggtggacgac gtattcgggc atcagggtgg tgatctgccc ggcgagctcg atgaagcggg
       61 tggccatgcc gtattcgcgg gctttccagc tcagatagaa cgggtcgatg ggaatgcagt
      121 ggccccccag acccggcccc ggatagaaag gcatgaaccc gaagggcttc gtggccgccg
      181 cgtcgatgat ttcccacacg tcgagcccca tgcgggtgca gagaagtttc aattcgttga
      241 ccagggcgat gttgacgctg cggaagatgt tctcgagaag tttcgacagc tcggcgacct
      301 cggggctgct ggcgcggaca atgcgcttga agatggtcgc ataatattgt gccgcaagct
      361 ccgcgcaggc gggcgtcacg cctccaagca ccttgggtat gttgctgatg gaaaactccg
      421 ggttggctgg atcttcgcgt tccggtgagt aggccaggaa aaagtcctcg ccggccttca
      481 atcccgtgtt ttcgaagaga cggaggagga tctcggttgt cgtgccgggg taggtggtgc
      541 tttcaaggag caccagctgg ccggggcgca gggttttggc gatgctttcg gcggtggcac
      601 gcacgtactg gaggtttggc tccaggtgct catcgagagg cgtgggcacg cagatgctga
      661 tgcaatcagt ttcggcgagg cgtttaaagg ccgtggtagc ggaaaacgaa ccgttcgtga
      721 cggccgcgcc gatccacccg gaatcgatgt gtttgatata gctctctccc cggttgatgc
      781 gctcggtttt ctcaggatcg acatcgaacc ccagtaccct gtatccggcg ccgcacaggt
      841 ggcgcgcgag cggcaacccg acgtagccca acccgatcac cccgccgacg caggctttgg
      901 ccgcgatctt ccggctcaat tctttggcca gaggtggcag ttccattggc atccctccgc
      961 tgtgacgctt ccggctcggc cccataaacg cgaacgggtg aaattatgac acagaccccg
     1021 caaagccgcc atgcccaagc gagaatgctc cgggagcttc gctcaggctc ttcggcggtc
     1081 tcgacttggc gcgctgccgc gcgtatcctg tgaatccgtc acggatatac tgagtgccgg
     1141 tctgaagcct ggggcatcag ggagccgcct ggcagcgccg ctgccccggt gggaatgagc
     1201 gaggggagag cgtaggtatg aaacgcgtga gcatcgtact tggcgtgggg attctgttgg
     1261 ggctgtgcgc ccggggcgct gaactgccgg cgccgcccgc gccgctgacc ggacaggcct
     1321 tgccggggat gccgtttacc acctccgagg acgcgcgcgc ggcgttcaag gccgtcggca
     1381 acaccgcccc cgtcgatgtg gttgaagcgg gcggccgcgc catgttgcgc atgccctgcg
     1441 tttttgaggg ggtggagacc gagcgggcgg tatgggactg gcgcggccag ctggacctga
     1501 cccggagcca ggggctgcag ttttactttt attctcccga cacctcgccc gtttcgtatt
     1561 tttccctgta tctgcgcagc ggcgacggct ggtaccacat gaccttcggg gccaaggcgc
     1621 ccggccagtg gacccccgtc gtgttcgaca aatccgccac ggacctggaa ggccggccgc
     1681 agggttgggg cagcattgat ctcgtccgca tcgccgcctg gcggggaaag caccagaata
     1741 ccgagtttta tgtcgcggac ctgtcgctct atggcgccga tgcccccatc ggcatcgtgc
     1801 ggaacgaatc cgccgccgct gacaagaaca gcgagtacaa gatcgcggcg cagtgcaccg
     1861 ggtttgtggc cgaacagctc ggggcgctcg gcataccgtt catcatcatg agcgacctcg
     1921 atttgtcgaa cgctttcctc cgcgacaagc agctgatcat cctgccgtac aacccgtcca
     1981 tgtccgacgc ggcggtgcag gcgttggcgc gcttcctgga gcgaggcggc aaattgatgt
     2041 cgttctaccg gctcgattcg cggctcgccc cgctcatggg cgtgaaatca gggcggtacg
     2101 tgggacgcga ccagaatgta accttcggat ccatccgcgc ggccggcacg ccgctcgaag
     2161 ggcagccggc ggaggtcacc cagcattcat ggaacattca ggacgtgctc ccggttgacg
     2221 gaaagagccg cgcggtggcc gcgtggtacg atgaagcggg cctgcccacg ggctgcaatg
     2281 cgatcgtcgc ctcggccaac gcggtgcaca tgagccacat tctgctcacc gaggatccgg
     2341 tgcgaaaacg catgctgctg ctggcgatga cggggcatct tctgccggac g
//
LOCUS       DAOPBQ010000548         2389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_34913_length_2389_cov_3.484147, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000548 DAOPBQ010000000
VERSION     DAOPBQ010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34913_length_2389_cov_3.484147"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKW60_13980"
     CDS             complement(<1..382)
                     /locus_tag="PKW60_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK00590.1"
                     /translation="MKRISSLYLFFGAEIVIAASMGINESIFNNFLSDTFELTAEARG
                     ALEFPRELPGFLVVLFAGILGALSLPRMGALAALVFAAGTAGLGLAGNSYGLVVASML
                     VSSVGMHLNQPISHSLTIAMSEDDR"
     gene            complement(410..1714)
                     /locus_tag="PKW60_13985"
     CDS             complement(410..1714)
                     /locus_tag="PKW60_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00591.1"
                     /translation="MGQSRESNVVFILNALVFALVAVFGVLGSLAITPARHDLALSRL
                     ASLYSLTEYGSFYLDAPAGKEPNPFASTTVDKVLARGREISSKPPVLPLLMTAEYMAL
                     RRVAGLDLKNEADNGRIIRVLTFTFVSLPFFLTILLFDRTVALFDVEPLIRWVVTFFL
                     AFGTQLWGYSLSINNHIPGTFFLMLCLYLALGLIYGKWAPSGWRFALFGLSAGLVLTV
                     EMPAAIWAAIAGLCLLYHFPRKTLVWGALAVAVPVAVHVGVMLHVTGSPLPVQMHPEM
                     YLFENSYWRHPTGIDALNEPKGTYLFHMTFGRCGLFILYPMLLAGLLSPLRYLFGKPL
                     RHHRPILLGALGFLILTAYYCLQTNNYGGVLYGFRWYIPAMPVLMLMGVPVYEKLRAR
                     WEWIVVGLMFGISFFSAWEATRGTAYSGQEWPCQFLGPSTPS"
     gene            complement(1817..>2389)
                     /locus_tag="PKW60_13990"
     CDS             complement(1817..>2389)
                     /locus_tag="PKW60_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00592.1"
                     /translation="CGRFGGRTEKDLSMTAPEAADTQRHEMSAAAKEQRLRDTVEELV
                     ADAARIDTPERAPIIKREPYFLKEYSLYPTGAASMKLQIHQTQSRVAPLVGEAVIPKQ
                     RFATEMHRKKDAAREDSFFYRETGNEILTYQWRNGRWVQVASLFVVDVKEERDGDGWR
                     AVEPQAKEELLLKDEDEGFIRRMFGGIFGR"
BASE COUNT          533 a          748 c          684 g          424 t
ORIGIN      
        1 gtctgtcgtc ctcgctcatg gcgatcgtga gcgaatgcga tatcggctga ttcaagtgca
       61 tgccgacgct gcttaccagc atcgaggcga ccaccagccc gtacgaattc cccgccaatc
      121 ccaggccggc cgtgcccgcc gcaaagacca gagcggccag cgcccccatg cgcggaagcg
      181 agagcgcgcc taaaatcccg gcgaacagta ccacgagaaa ccccggcagt tcgcgcggga
      241 attcgagggc gccccgtgct tctgcggtca attcgaaggt gtcggagagg aaattattga
      301 agattgactc gttgattccc atggaggccg cgatgacgat ctcggcgcca aaaaacagat
      361 agagcgagga aattcgtttc acgagacctg atccaccgcg aatcgcgggc tacgaggggg
      421 tgctgggccc caggaattgg cagggccact cttgaccgga atacgcggtc ccccgggtgg
      481 cctcccacgc ggaaaaaaac gagatgccga acatgaggcc aaccacaatc cattcccacc
      541 gggcgcgtaa cttctcatag acgggcaccc ccatcagcat caacaccggc atggcgggaa
      601 tgtaccaccg gaacccatag agcacgccgc cgtagttgtt cgtttgcaga caataatagg
      661 cggtgagaat cagaaagccc agcgccccga gcagaatcgg ccggtgatgc cgcaacggtt
      721 tgccgaacag gtaccgcagc gggctcagca gccctgcgag cagcatggga tacaagatga
      781 acaggccgca gcgcccgaag gtcatatgga acaaataggt gcctttcggc tcattcaacg
      841 catcaatgcc ggtgggatgc cgccagtacg agttttcgaa aaggtacatc tccggatgca
      901 tctgcacggg gagaggactg cccgttacgt gcagcatgac gcccacatgc accgccacgg
      961 gcacggctac cgccagcgcc ccccatacaa gcgtttttcg aggaaaatga tacagcaggc
     1021 acaagcccgc gatggccgcc cagatggccg ccggcatttc cacagtcaaa accaggcccg
     1081 ccgacagtcc aaagagcgca aaccgccagc cggaaggcgc ccacttcccg taaatcagtc
     1141 ccagggccag gtaaaggcag agcatcagga aaaatgtccc gggaatgtgg ttgttgatcg
     1201 agagactgta accccagagc tgcgtcccaa aggccaggaa aaacgtcact acccaccgga
     1261 tcagcggctc gacgtcaaac agcgccaccg tgcggtcaaa cagcaggatc gtcaagaaga
     1321 acggcaggct cacaaacgtg aacgtcagca cgcgtataat tcgcccattg tccgcctcat
     1381 ttttcagatc caacccggct acccggcgca gtgccatgta ctctgccgtc atcaacaggg
     1441 gaaggaccgg aggtttgctg ctgatttcac ggccccgggc caggaccttg tcgaccgtag
     1501 tcgacgcaaa agggttgggc tccttccctg ccggtgcatc gagataaaaa cttccgtact
     1561 cagtcaggct gtacaggctg gccaggcggc tgagcgcgag atcatgacgc gcgggggtaa
     1621 tggcaagcga cccgagcact ccaaacacgg ccacgagggc aaagacgagg gcattcaaaa
     1681 tgaatacgac gttggattcg cgggactggc ccacaatacc ttctcccggt ggtttatcag
     1741 gccgcgttgt tcttcattcc gagggttcca ggcggcgtgc cgaaagcgtc ggtctgttgc
     1801 cgctacggcg cgcggatcag cgcccgaaaa tgcctccaaa catccgccgg atgaagccct
     1861 catcttcatc tttcaggagg agctcttctt tcgcttgcgg ctcgacggca cgccaaccgt
     1921 ccccgtcccg ctcttccttc acgtccacaa caaacaggct ggcaacctgc acccaccggc
     1981 cgtttcgcca ctggtatgtc aggatctcgt tccctgtttc gcggtagaaa aaggagtctt
     2041 cccgtgccgc gtcttttttg cggtgcatct cggtcgcaaa gcgctgtttg ggtataacgg
     2101 cttcacccac caggggggcc acccgcgatt gcgtctgatg gatctggagt ttcatgctcg
     2161 ccgcacccgt gggatacagc gaatactcct tcagaaaata cggctcgcgt ttaataatgg
     2221 gcgcgcgttc tggcgtgtca attcgcgcgg cgtcggccac gagttcctcg accgtgtcgc
     2281 gcaatcgctg ttccttcgcc gcggcagaca tctcgtggcg ctgcgtgtcg gcggcctccg
     2341 gcgcagtcat cgaaaggtct ttctctgtcc tgccgccgaa gcggccgca
//
LOCUS       DAOPBQ010000549         2370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_35310_length_2370_cov_3.447084, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000549 DAOPBQ010000000
VERSION     DAOPBQ010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35310_length_2370_cov_3.447084"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1243)
                     /locus_tag="PKW60_13995"
     CDS             complement(89..1243)
                     /locus_tag="PKW60_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HPK00593.1"
                     /translation="MESATIQNTHENPPAHPPAARPERLASIDALRGFDMFWIIGGNP
                     LVLAFLGMFFDPVPQWLEYHFEHVKWEGFSAWDLIMPLFLFVSGVSLPFSLAKWTGSS
                     GKGKDRKALYIRLARRILLLWVLGMLVQGNLLRCDLSRLHLYSNTLQSIATGYLVATL
                     LLLFCSIRGQLAAAVALLLGYWGLMVLVPVPGHGAGVLEPHANLALYIDEVILGRFRD
                     GTTYTWILSGMGFAVSVLLGVFGGHVLRSDQTKNRKAAILFASGIGCLAAGWIWGLAF
                     PIIKHIWTSSMVLWAGGWSYLLLALFYWLIDVKQYRKWAFPFIVIGMNAIAVYVATSL
                     ISFRRISEDLVGHIAEHLGVAGPFLVTLTAFMIVWLILLYLYRKGTFIRA"
     gene            complement(1476..>2370)
                     /locus_tag="PKW60_14000"
     CDS             complement(1476..>2370)
                     /locus_tag="PKW60_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HPK00594.1"
                     /translation="TFPIWNLPLKHPVNMAYEAATADLKDVNLIDPFHLEMYGRTAVN
                     YNRDVEAFPVLKRILERIMGAETCYRSPTDMSVNRVGFGIIDEKRAQEAATQEVIRRV
                     LRYRCEFMMGLASRDTVERAELIMNELGAQIEDRRMVQPARDAAKRAEAGQKGHDGVY
                     CGAAIELPDGAILTGSNSPLMHSTASAVLNAIKHLAEIPDKIHLIAPNIINAIGNMKR
                     DIKDGKSISLNLEEALIAVGASAPFNSAAQIAIEQLVNLRGCDMHTTHIPTPGDEAGL
                     RTLGVNLTSDALFASDNLYLA"
BASE COUNT          500 a          662 c          739 g          469 t
ORIGIN      
        1 cacacaggac gacccttttg cgtagatcct gcgaggcagg gcatccccca catcgcctat
       61 cggcacagac aacagcggtc tgctggattt acgcccggat gaatgtgcct tttcgataga
      121 gatagagcag gatcagccag acgatcataa acgcggtcag ggtgaccagg aaggggccgg
      181 ccacgcccag gtgctccgcg atatgcccga ccaggtcttc gctgatgcgg cggaaactga
      241 tcagagaggt cgcgacgtac acggcgatgg cattcattcc gatgacaatg aaggggaacg
      301 cccatttgcg gtactgtttc acgtcgatga gccaataaaa gagggcgagc agcagatagc
      361 tccatccgcc ggcccaaagg accatcgaac tggtccagat gtgtttgata atggggaacg
      421 ccaggcccca gatccagccg gcggccaggc agccgatgcc gctggcgaag aggatggcag
      481 cctttctatt cttggtctga tcggagcgca gtacgtgccc tccgaatacg ccaaggagta
      541 cgctcacggc gaagcccatg ccgctgagta tccaggtgta ggtggttcca tcgcggaacc
      601 ggcccaggat gacttcatcg atgtagaggg cgaggttggc gtgcggctcc aggactccgg
      661 cgccatgccc gggaacgggc acgagcacca tcaggcccca gtatcccagg aggagagcca
      721 cggccgcggc aagctgcccc cggatggagc agaacaacaa cagaagggtg gccacgagat
      781 agccggttgc gatggattgc agggtatttg agtacaagtg cagccgggac agatcacatc
      841 tcaggaggtt tccctgcacc agcatgccga gcacccacag cagcagtatc cggcgagcga
      901 gccgaatata gagcgctttc cgatctttcc cctttccgct ggaaccggtc cacttggcaa
      961 gtgaaaacgg cagggagacg ccgctcacaa acagaaagag cggcatgatg aggtcccagg
     1021 ccgaaaatcc ttcccatttg acatgttcaa agtgatattc gagccattgc gggaccggat
     1081 caaagaacat gcccagaaac gccaggacca gcgggttgcc gccgatgatc cagaacatgt
     1141 cgaagccccg cagggcgtcg atcgaggcga gccgttcggg ccgcgcggcg ggcggatggg
     1201 cgggtgggtt ctcgtgcgtg ttctggatgg tggcactttc catgattctt ccccagtttt
     1261 tcgcagcggt gcagcgccac gattggttca cggcgtctgg tattcccgaa atacaggttg
     1321 ccatggacgg cctgctccac tgggcaacag gcctccccct ggcgggctgt acattaagga
     1381 aaacatagta tccacaagcc cgcaaatgtc gtcaactttt cgaggcacaa acgccccggt
     1441 tccagaacgg ccgattccac agggaaaatg cacgcttacg ccaggtagag attgtccgaa
     1501 gcgaagagtg catcactggt gaggttcaca ccgagcgttc tcagtccggc ctcgtcgccc
     1561 ggggtgggaa tgtgcgtggt gtgcatatcg caaccgcgca ggttcaccag ctgttcgatg
     1621 gcgatttgcg cggcgctgtt aaagggagcg gaggcgccga ccgcgatgag cgcttcctcg
     1681 agattgaggc tgatcgactt gccgtccttg atgtcgcgtt tcatgtttcc aatcgcgttg
     1741 atgatgttcg gggcgatgag atgaattttg tccgggatct ccgcgagatg cttgatggcg
     1801 ttgagcacag cgctggcggt ggagtgcatg aggggcgaat tgctgccggt gaggatggcg
     1861 ccgtccggga gttcgatggc agcgccgcaa tagaccccgt catgaccttt ttgcccggct
     1921 tcagcccgct tggcggcgtc ccgggccggc tgtaccatac gccggtcttc gatttgtgcg
     1981 ccgagctcgt tcatgatgag ttcagctcgt tccacggtat cccgtgaggc aaggcccatc
     2041 atgaattcgc atcggtagcg gagcactcgc cggatgactt cctgcgttgc ggcttcctgc
     2101 gctctctttt catcgataat gccgaaaccg actcgattga cactcatatc ggttggggag
     2161 cggtagcagg tctccgctcc catgattcgc tcgagaatgc gtttaaggac ggggaaggcc
     2221 tctacatcac ggttgtaatt gacagccgtc cgcccgtaca tctcgagatg gaacggatcg
     2281 atcagattca cgtccttaag gtcggcggtt gccgcctcgt aggccatatt cacagggtgt
     2341 ttgaggggaa gattccagat ggggaaggtc
//
LOCUS       DAOPBQ010000550         2364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_35452_length_2364_cov_2.613252, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000550 DAOPBQ010000000
VERSION     DAOPBQ010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35452_length_2364_cov_2.613252"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="PKW60_14005"
     CDS             <1..1377
                     /locus_tag="PKW60_14005"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HPK00595.1"
                     /translation="AVSIPEAVLQGIWYEQLLRRDGLSLADGRSLRVIFPGWWNQGDG
                     PDFRDAQLEIAGDYVAGDVEIHQDPGAWRQHGHHQDPRYDRVVLEVIRNICNGDTTNV
                     TSGGRRIPCLSLEPALIDDIDVLAGQIQARDYPYRVPASYGECARLTAMHGVEPMVRL
                     LRLAGEWRMLAKARELTQRMERASDDQAVYEAVMAACGYGPYKQQFRALAQQLHYERI
                     RQLGIQDPFLVEAAFLQLAGLLPEALPENAGDVPHFQRLKNLREQRLRGLRRLPMRWE
                     RNGVRPNNYPERRLAGAARFFGRTAKPGLSAALEAIWQDEVKPVARRRAFEALFPSAM
                     GFWAERCTWTGKPLEKPVAMLGQGRVHAIIGNVFVPVALARARREHNPVLEARVFDFF
                     TVLPKEMDNHVVKTMISRLFGATPPPRLTFQMQQGLLQLYTDWCRFNPKCGACPALAL
                     LNLVGEQE"
     gene            complement(1492..>2364)
                     /locus_tag="PKW60_14010"
     CDS             complement(1492..>2364)
                     /locus_tag="PKW60_14010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPK00596.1"
                     /translation="EAVRQALAKGKELHQRFIGKTSHGTPLKCEILAQPLGEGPNTQG
                     QLLLIIDISEQLELQRNLQQSEKLYTLGELVAGVAHELNNPLTSIIGRTELLMRGNLE
                     TKVRDGLAQISEGAERCRRIVENLLSFGSHHEARKGLHRVNQIIADTLQLREYQMRVS
                     GITIETEFDDANPPLLMNPNEMRRVFFYLITNAHQALEPIRGRPRKLRITTALVGDTA
                     QIRFEDNGHGIPAEIQSRIFDPFFTTRKIGEATGLGLSVSYGIINEHGGSIQMESEEG
                     EGATFTVTLPLPDN"
BASE COUNT          426 a          674 c          772 g          492 t
ORIGIN      
        1 gccgtctcga tacccgaggc ggtcctccag ggaatatggt atgagcaact cctgcggcgc
       61 gacggccttt cactcgccga tggacggagc ttgcgcgtga tttttccggg ctggtggaat
      121 cagggcgatg gcccggattt ccgtgacgcg cagctggaga ttgctggtga ctatgtggcg
      181 ggagatgtcg aaatccacca ggatcccggc gcgtggcgcc agcatggaca ccatcaggat
      241 ccgcgttacg accgcgtggt actggaagtc attcggaaca tctgcaacgg tgacaccacg
      301 aatgtcacgt cgggcgggcg ccgcatcccc tgtctttctc tcgagccagc attgatcgat
      361 gatatcgacg tgctcgccgg acagatccag gcgcgggatt acccttatcg cgttccggca
      421 tcgtatggag aatgtgcccg cctgacagcc atgcacggcg tagagcccat ggtgcgtctg
      481 ttgcgcctgg ccggcgagtg gcggatgctc gcaaaagccc gggagctgac ccagcgtatg
      541 gagcgcgcga gcgatgatca agccgtgtat gaagcggtga tggcggcctg tggttatggg
      601 ccgtataaac aacaatttcg cgctctggct cagcagctgc actacgaacg gatccgccag
      661 ctgggtatcc aggacccgtt cctcgtggaa gcagcttttc ttcaattggc agggctgttg
      721 ccggaggctc tgccggaaaa tgcgggcgat gtgcctcatt ttcaacgtct caaaaacctc
      781 cgggagcagc gattgcgcgg gttgcgccgc ctgcccatgc gttgggagcg aaacggcgtc
      841 cgtccaaata attacccgga gcggcgtctg gccggcgcag ctcgattttt cggccggacg
      901 gcgaaaccgg gcttgtcggc cgccctggag gccatctggc aggacgaggt caaaccggtc
      961 gcccggcgcc gggccttcga ggctttgttt ccctccgcga tggggttctg ggccgagcgg
     1021 tgcacctgga caggcaaacc tcttgaaaaa cctgtagcga tgctcggaca ggggcgtgtg
     1081 catgcgatca tcggcaacgt gtttgtgcca gtggcgctgg cccgggctcg ccgtgaacac
     1141 aatccggtcc tggaggcacg ggtgttcgat ttcttcaccg tgctgccgaa agaaatggac
     1201 aaccatgtgg tcaaaacgat gatctcgcgg ctgttcggcg ccaccccgcc cccgcggctc
     1261 acatttcaaa tgcagcaggg attactgcag ctgtatacgg actggtgccg cttcaatccg
     1321 aaatgcgggg cctgcccggc tctggcgctg ctgaatctgg tgggagaaca agaatgacag
     1381 acaatccccg gtcagatttc cggcgcctgc aggcggcgga tgcgttcatc aaggagcccg
     1441 tgcgtcgcgc ccggtagtaa gggctgagcg ggccaggcag gcgccccggc ctcagttatc
     1501 cggcagcggc agcgtcacag tgaaggtggc gccttcgccc tcttcggatt ccatctgaat
     1561 ggagccgccg tgctcattga tgatgccgta ggacacgctc aaaccgagtc cggtggcctc
     1621 gccgattttg cgggtggtga agaacgggtc gaaaatccgc gactggattt cagcagggat
     1681 gccgtggccg ttatcttcga agcggatctg ggcggtatcc cccacgagcg ccgtggtaat
     1741 gcgaagtttg cggggacggc cccggatggg ttccagcgcc tggtgagcgt tggtgatgag
     1801 gtagaaaaag acccgccgca tctcgttggg attcatcagc aacggtgggt tggcgtcgtc
     1861 gaattcggtc tcaatggtga tgccgctgac gcgcatctga tattcgcgaa gctggagcgt
     1921 gtcggcaatg atctggttaa cgcgatggag acccttccgt gcctcgtgat ggctgccgaa
     1981 tgagagcaga ttctcgacga tccgcctgca gcgttcagcg ccttcgctga tctgggcaag
     2041 cccgtctcga actttcgtct cgagattgcc ccgcatgagg agttcggtgc gaccgatgat
     2101 gctggtcagc gggttattga gttcgtgggc caccccggcg accagttccc ccagggtgta
     2161 cagcttttcg gactgctgca aattacgttg caattcgagc tgctcggaga tgtcgatgat
     2221 cagaagcagt tgcccttggg tattggggcc ttcacccagc ggctgggcga gaatttcgca
     2281 tttcaggggc gtgccgtggc tggtctttcc gatgaaccgc tgatgcagtt ccttgccttt
     2341 cgccaaagcc tggcggacgg cctc
//
LOCUS       DAOPBQ010000551         2362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_35483_length_2362_cov_3.258778, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000551 DAOPBQ010000000
VERSION     DAOPBQ010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35483_length_2362_cov_3.258778"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PKW60_14015"
     CDS             <1..265
                     /locus_tag="PKW60_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00597.1"
                     /translation="SPEESWRECHLHARNLLGKGGYEALLAELAGETTPQAATAKTPT
                     TSSPNTKPPASHDAYGQLHLGGRGFDEDIPLLQEKDEDVREEQ"
     gene            462..674
                     /locus_tag="PKW60_14020"
     CDS             462..674
                     /locus_tag="PKW60_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00598.1"
                     /translation="MAINKDSPKAKQGAERLPQDLRDVYWRMVEEYAFLTKVRYGRGY
                     VAYEILADMVLAGWRPSAERHPESRI"
     gene            711..>2362
                     /locus_tag="PKW60_14025"
     CDS             711..>2362
                     /locus_tag="PKW60_14025"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPK00599.1"
                     /translation="MARSDYLASLNDEQRVELQKRLWERQSGTCFICEDPIDLRLHKG
                     QLDIDHIDPLAEDGLDAENNFALTHASCNRSKGASDLRVARRLAEFERLQQNAHKAGK
                     RGANLGDVLERHGGAKYPLRIKRNGESVEFTFSASGDHTIRSVPLYTDKLSDMPYFFA
                     NVPIEYIHHDDRINPRSIGPNIRGLIEEFIKKRPQLHVGLCWWAPDTDSAGPLKLFDG
                     QHKTAAQILLGVRELAVRVFVKPNINVLLQANTNAGGKLRQVAFDAAVMRHLGSTLYA
                     ERVRQYRSMRALAEDDLSFSEQDLVRFFRGERREMERYIIDAQRDNITHSTENKLLEF
                     VEWAGKVAARPISYSTVEKTFFKEFLYKQALDTPIEEGLEQGTNCRLVEREQMVRLMS
                     LFAQVCFIGKWDPEIGGRRLENRVQKGEKIDENHLRAWRMAREEVVDNVVRWIRLVIE
                     NYFSWTGKMIDRERLLHTPLPDDLWRRIEVFLHNLAQLPCWVDRNLSTTVFGPKQNPE
                     FWDKVFSTGVATNGERILAEGLDLHEMIKEKPLTGYGAGNWE"
BASE COUNT          580 a          602 c          705 g          475 t
ORIGIN      
        1 gtctcccgag gaatcctggc gcgaatgcca cctccacgcc cgcaatctgc tgggcaaagg
       61 gggctatgag gcgctgctgg ctgaactggc gggcgaaacc actccacagg ccgccacagc
      121 caagacccca accacctcat caccaaacac caaaccaccc gcctcgcacg atgcctacgg
      181 ccaactccac ctcggcggcc gcggctttga tgaggatatt ccgttgttgc aggagaaaga
      241 tgaggatgtc cgggaagaac agtagccctg aaaaatgcgg cgcaggtgcg gccactccag
      301 aagtgacgat caggcatcac ggagtttgca gcagaccctg ggggcgtgaa gcttgcttcg
      361 cgaaaagaaa agagatgaag cgcgcccact ggatcctggc cggaaggctt ggtatccgca
      421 ttgagacacc agcataatgg ggcagtaaag ggtaggaacg aatggccatc aacaaagaca
      481 gcccgaaagc aaagcagggg gcggaacggc tgccgcagga cctgcgcgac gtgtactggc
      541 gtatggtgga ggaatacgcc ttcctgacga aggtgcgcta cggccgcgga tacgtggcct
      601 acgagattct ggccgacatg gtgctcgcag gctggcgccc cagcgctgag cgccatccag
      661 aaagcaggat atagaagtaa ttcggaacaa gcccattggg agaacacgaa atggcgagat
      721 cagactatct tgcgtcactg aatgacgagc aacgggtaga attacagaag agattgtggg
      781 agaggcagtc gggaacctgc ttcatctgtg aagatccaat cgatctcaga ctgcataagg
      841 ggcaattgga tatcgaccac atcgatccac tggcggaaga tggcctggat gcggagaata
      901 attttgcgtt gactcacgca agctgcaatc gcagcaaggg ggcttcggat ctccgcgtag
      961 cccgccgcct ggcggaattc gagagactgc aacagaatgc ccataaggct gggaaacgtg
     1021 gcgcaaacct cggggatgtt ctcgagcgcc atggtggagc gaaataccct ctccggatta
     1081 agcggaatgg cgagtcggtc gagtttacgt tcagcgcctc gggagaccat acgattcgat
     1141 cggttcccct ttatactgac aaactcagcg atatgcccta cttcttcgcg aacgttccta
     1201 tcgagtacat tcaccatgac gatcggataa atccgcgcag catagggccg aatatccgcg
     1261 gtttgataga ggaatttatc aagaaacgcc cccaattaca cgtcggcctg tgttggtggg
     1321 cccccgacac ggatagcgct ggcccgttga agttgtttga cggtcaacac aaaacagccg
     1381 cccagattct actgggtgtc cgcgagttgg ctgttcgtgt ctttgtaaag ccaaatatca
     1441 acgtcctgct tcaagccaat accaatgcag gcggcaaact gcgtcaggtg gctttcgatg
     1501 cagctgttat gcggcatctg ggcagcacct tgtatgcgga acgtgtccgg cagtatcgga
     1561 gtatgcgggc tcttgcggaa gatgacttgt cgtttagtga gcaggatctg gtgcggttct
     1621 tccgcggtga acggcgcgaa atggagcgtt acattattga tgcccagcgg gacaatataa
     1681 cccatagcac ggaaaataag cttctggagt tcgtagagtg ggcgggcaag gtggctgcgc
     1741 gtcccatttc ctattccacg gtcgaaaaga ccttcttcaa ggagtttctc tacaagcagg
     1801 ctctggatac cccgattgag gaaggactcg aacagggaac aaattgccgg cttgtagagc
     1861 gggaacagat ggtgcgcctg atgtcacttt tcgctcaggt gtgcttcatc ggcaaatggg
     1921 atcccgaaat cggcggcagg cgcttggaga accgagtgca gaagggtgag aaaatcgacg
     1981 agaaccacct tcgggcatgg cgtatggccc gcgaagaggt agtcgacaat gttgtgaggt
     2041 ggatccgact ggtgattgag aactacttct cctggaccgg caagatgatc gacagagagc
     2101 ggctgttgca tacgccgctt cccgatgact tgtggcggcg tatagaagta ttcctccata
     2161 accttgcgca gcttccctgc tgggtagacc gtaatctcag cacgactgtc tttggcccga
     2221 agcagaatcc ggagttctgg gataaggtct tctccactgg tgtggcaaca aacggcgagc
     2281 gcattttggc tgagggtttg gaccttcatg agatgatcaa agaaaaacct cttactggtt
     2341 atggtgcagg taactgggaa at
//
LOCUS       DAOPBQ010000552         2356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_35591_length_2356_cov_4.152542, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000552 DAOPBQ010000000
VERSION     DAOPBQ010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35591_length_2356_cov_4.152542"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(305..766)
                     /gene="smpB"
                     /locus_tag="PKW60_14030"
     CDS             complement(305..766)
                     /gene="smpB"
                     /locus_tag="PKW60_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPK00600.1"
                     /translation="MGEKTIVQNRRARHDYHILERFETGIALQGTEVKSLRAGQITLK
                     DSYAEVQDGEIFLVGVHIAPYEQGNIYNHDPERRRKLLMHKREIIRIGHSVAEKGFTL
                     VPLRVYFKEGRAKVELGLCRGKQTVDKRQTLRKREQDRDIDRALKDLKRNK"
     gene            complement(875..2173)
                     /gene="tolB"
                     /locus_tag="PKW60_14035"
     CDS             complement(875..2173)
                     /gene="tolB"
                     /locus_tag="PKW60_14035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HPK00601.1"
                     /translation="MKIWCLTVIAAMFAPLAFGQVEIGTTGQAEGRIPIAVPDFCTAP
                     GQEALGRAMAQVLAYDLYFSGVFGIVPPNAYPRAFTGFTRDPQTIDFEAWRKTPAEFL
                     VHAYVTSDAQSLTAECRLFDVLSGEMVSGKRLATRGQEWHRLVAHQFADEIVRHLTGI
                     AGCASSRICFSGGSTRKKEIYVADYDGANLKQVTQHGSISILPKFSPDGRKIAYLSYK
                     DRYPFLYVLDLDTGESRPLSKQVGLNVAPAWSPDSRQLAIVQSKDGNNEIYLVNADGS
                     NPRRLTHDRALDTSPAFDPSGTRIAFVSERGGAAQIYVMATDGSGVKRISYQGGRAYD
                     PVWSPDGTKIAYVVEKSGDGFEIYVMAADGSGAKRLTNSVGNNESPSWSPDSRHVMFA
                     SSRSGRWELGAVNVETGEEQRIAVNAELSLQGPSWGPRRQ"
BASE COUNT          409 a          672 c          773 g          502 t
ORIGIN      
        1 ttaaacggcg atgtgatcga ggccgccgcg ccaagaccga gagcggtcga caggaagttt
       61 cggcgagact gattctgcat ggtgacttct ccttaatgca tctacatacg ttcgcgcgcc
      121 ggttcgcacc gtttctggac cggttcaggc cgtgcgcgcg catccatttc gcaccgtccg
      181 gtttgttgtg gggcggaaag acattccttt gcctccggcc gccaccttcc ttgttatcat
      241 gccgaagcag tccggagcaa attttcgtcc tcagcgcgct catacagcct ggccggacca
      301 aagctcattt gttccgtttg agatccttga gggcgcggtc gatgtcgcgg tcttgttcgc
      361 gtttgcggag ggtctgccgt ttatcgacgg tttgtttgcc gcggcagagg ccgagctcga
      421 ctttggcgcg cccctctttg aaatagacgc ggagcggcac gagggtgaac cctttttcgg
      481 cgacgctgtg gccgatgcgt atgatctcgc gcttatgcat caggagtttt cggcggcgtt
      541 ccgggtcgtg gttgtaaatg ttgccctgct catacggagc gatgtggacg ccgacgagga
      601 agatctcgcc gtcctgcacc tcggcgtagc tgtctttcag ggtgatctga cccgcgcgga
      661 gggactttac ctcggtgccc tggagggcaa ttccggtctc gaagcgttcg agaatatggt
      721 aatcgtgccg cgcgcggcga ttctgtacga tggttttttc gcccatgatg gttccagtgt
      781 ggcgcaaacg aaacgggttt tcaacgcatg ggagcgggat gcgccgcgac agcccgcacc
      841 tctcctttat tcgcgttgcc ctggcgtgcg gctactactg cctgcgcggc ccccacgacg
      901 gcccctggag cgagagttcg gcgttgacgg caatgcgctg ttcctcgccg gtctcgacgt
      961 tgacggcgcc cagttcccag cggccgctgc ggctcgaggc aaacatgacg tggcgtgaat
     1021 cgggggacca gctgggcgat tcgttgtttc ctacggaatt ggtcaagcgt tttgcgccgg
     1081 agccatcggc ggccatgacg tagatctcaa agccgtcgcc ggatttctcg acgacatagg
     1141 cgattttggt gccgtcgggc gaccacacgg ggtcgtaggc tcgcccgccc tgataggaga
     1201 tgcgttttac gccggaccca tccgtggcca tgacgtagat ttgcgcagcg ccgccgcgct
     1261 cgctgacgaa ggcaatgcgc gtgccggacg ggtcaaacgc gggcgaggta tcgagcgcgc
     1321 ggtcgtgggt gaggcgccgc gggttcgagc catccgcgtt gaccagatag atctcgttgt
     1381 tgccgtcttt actctgcacg atggcgagct gacggctgtc gggcgaccag gccggggcca
     1441 cgttgagtcc gacctgcttg gacagtggcc gggattcgcc ggtgtcgaga tccagcacgt
     1501 acaggaaggg gtagcggtct ttatacgaca ggtaggcgat ttttcggcca tccggggaga
     1561 atttggggag gatcgagatg gagccgtgct gggtgacctg tttcaggttg gcgccgtcgt
     1621 aatcggcaac gtagatttct tttttcctcg tgctgccgcc gctgaaacag atgcgcgacg
     1681 acgcgcagcc ggcaatgccg gtgaggtgcc gcacgatctc gtcagcgaac tgatgggcga
     1741 cgaggcggtg ccattcctgg ccgcgcgtgg cgaggcgctt accgctgacc atctcccccg
     1801 aaagcacgtc gaagaggcgg cactcagccg tcagcgattg tgcgtcggag gtcacgtagg
     1861 catgaacgag gaattccgcg ggggttttcc tccaggcttc gaaatcaatg gtctgcgggt
     1921 cgcgggtgaa tccggtgaag gcgcgggggt aggcattggg cggcacgatc ccgaatacgc
     1981 ccgagaaata gaggtcgtag gccagcactt gcgccatggc tcttccgaga gcctcctggc
     2041 cgggagcggt gcagaaatcc ggcaccgcga tggggatacg gccttcggcc tgtccggtag
     2101 tgccaatctc gacctgcccg aaggcgagag gggcaaacat cgcagcaata acagtgagac
     2161 accagatttt cacgatccgc ctcctatcgt accagggtga acgtgtaaat aacctgcact
     2221 tcgggttcca tgaaggtatc cggcaactga ggaaaaggtg tggccaattg cactgcgcgc
     2281 aacccggatt cggccagcgc ggcgtcattg gcatgctgca ccacttccgg tttgccgatc
     2341 aaattaccct gccggt
//
LOCUS       DAOPBQ010000553         2350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_35702_length_2350_cov_3.600436, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000553 DAOPBQ010000000
VERSION     DAOPBQ010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35702_length_2350_cov_3.600436"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..1039)
                     /locus_tag="PKW60_14040"
     CDS             complement(209..1039)
                     /locus_tag="PKW60_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015220028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPK00602.1"
                     /translation="MTRITVLVENTASKPGLLAEHGLAFWVEHAGHRILFDTGQSHAL
                     MHNARQLGIPLETADAVVLSHGHYDHSGGLAALFKKKSPLPLFLHPAALVPRYSRPSE
                     GSPREIGMPAPAVEAIRENTENVWTESPTEIFPGVHVSGSIPRRTAFEDTGGPFFLDD
                     AGQEPDLLPDDQALYLETRLGIVVVLGCAHAGVVNTVDYVRALTGARPIHTLIGGMHL
                     VSASEERMDQTVQALRHYRIERLMPAHCTGFPATARLWHEFPGVCVPCTVGAVVEIEE
                     "
     gene            complement(1089..1778)
                     /locus_tag="PKW60_14045"
     CDS             complement(1089..1778)
                     /locus_tag="PKW60_14045"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK00603.1"
                     /translation="MSTREDVSGHGLVDFGPMAWKYDRWYTTPEGMKHDAAQKADVLR
                     MLRSAGGPSRLLDLGCGTGHWSRFFASQGYSVTGVDLSMRMLQVAHKEGSTGVTCCAG
                     DAAALPFDDASFDVVAAMATLEFLPDPAGALREMARCVRRGGRILIGTLNRAAPLNRR
                     RLAESKQPYTSGRLLTPGALRELLEPFGPIRMAASDPDGTPGLEDREGTSKNLTDAGV
                     NGPFIVALVQR"
     gene            complement(1775..>2350)
                     /locus_tag="PKW60_14050"
     CDS             complement(1775..>2350)
                     /locus_tag="PKW60_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HPK00604.1"
                     /translation="IEFATADNGEWFVSETRCGPMVHARLGAGEENSGKLVNIVRTEA
                     RRIARQRGLETVLVDGSPGIGCPVIASITGANLVLVVTEPTLSGQHDLERVTQLPRHF
                     GIRTLVCINKWDLNPEVAEAIEAQATESGLRIAGRIRYDRGVTQAQIARLAVVEFQEN
                     GCAGDIRSVWQAVQDELHQLSGAPRNEGKAT"
BASE COUNT          412 a          756 c          739 g          443 t
ORIGIN      
        1 cacgtggagg ccgctgattc gaatcacggc gggtctctga gtggccatga ctacctcctc
       61 cgttcgggaa ccgccgcgcg ccggtatgcc tgcacatcgg ccaccgccgg cggcccttca
      121 ggaacgatgg cacggggcag tgcgttgtct atttgcccgc gggctgcccc cgggttttta
      181 tctcagcgtt cacgccgctg ctgcccggct attcttcaat ttccacgacg gctccaacgg
      241 tacacggcac gcatacgccc ggaaattcat gccacagacg ggccgtcgct gggaatcccg
      301 tacaatgggc gggcatcagg cgttcgatgc ggtaatggcg caatgcctgg accgtctgat
      361 ccatgcgctc ctcgctcgcc gaaaccaggt gcatcccgcc gatgagtgtg tgaataggac
      421 gagctcccgt cagtgcgcgc acatagtcca ccgtattcac gacccccgca tgcgcacagc
      481 ccaggacgac cactatcccg aggcgggtct ccagataaag ggcctgatcg tccggcagaa
      541 ggtccggctc ctgcccggcg tcatcgagaa agaaaggccc gccggtatct tcaaacgccg
      601 tccgccgggg aatcgaaccc gagacatgta caccggggaa gatctccgtg ggcgactccg
      661 tccagacgtt ttccgtgttc tctcggatgg cctccacggc gggtgcaggc atgccgatct
      721 cgcggggaga accttcagag gggcgcgaat acctgggcac cagtgcggcg ggatgaagga
      781 acagcggcag gggcgacttt tttttgaaca gagcggcaag gcctccgctg tggtcgtaat
      841 gaccgtggct caagaccacg gcgtcggcag tctccagcgg gatacccaat tggcgggcgt
      901 tgtgcatgag tgcatggctc tgcccggtgt cgaagagaat tcggtgtccg gcgtgttcta
      961 cccagaacgc aagcccgtgt tcagccagca ggccgggttt cgaggctgtg ttttcgacca
     1021 ggaccgtgat gcgagtcatg gcagaccttc ttttttaaag cggattccgc attttttgat
     1081 caggccgttc atctctgcac cagtgcgaca atgaaaggac cgttaacgcc cgcatcggtc
     1141 aggtttttgc tcgttccctc ccgatcttcg agtccgggcg ttccgtccgg gtcggatgcg
     1201 gccatgcgga tgggcccgaa cggctcgagc aactcacgca atgcgccggg ggtgaggaga
     1261 cggccggacg tgtacggttg cttgctctcg gccagccgcc ggcggttgag cggggcggcc
     1321 cggttcaggg tcccaatcag tatacgcccc ccgcgtcgaa cgcatcgagc catttctcga
     1381 agcgcgcccg cggggtccgg cagaaattcc agcgtggcca tggccgcgac gacatcaaac
     1441 gacgcatcgt caaacgggag tgctgcggcg tctcccgcac agcaggtcac gccggtgctg
     1501 ccttctttgt gggccacctg gagcatccgc atggaaaggt ccacacccgt tacgctgtaa
     1561 ccctgcgatg cgaaaaaacg gctccaatgg cccgtaccgc agcccaggtc caacaggcgg
     1621 gaaggaccgc cggcagatct gagcatccgg agcacatcgg ccttctgcgc tgcatcgtgc
     1681 ttcatccctt cgggcgtcgt ataccagcgg tcatacttcc aggccatcgg cccgaaatca
     1741 acgagcccgt ggcctgagac gtcttcccgg gtgctcacgt agcctttcct tcattgcggg
     1801 gtgcgccgga caattgatgc agttcatcct gaacggcctg ccatactgag cggatatcac
     1861 cggcgcaacc gttttcctga aattcgacga cagccagcct ggcgatctgc gcctgggtga
     1921 cgccgcgatc gtagcggatt cgcccggcga tgcgcaggcc gctttcagtg gcttgagcct
     1981 cgatcgcctc ggcgacttct gggttcagat cccacttgtt gatgcagacc agggtccgga
     2041 tgccgaagtg gcgggggagt tgggtcacgc gctccaggtc gtgctgaccg ctgagcgtgg
     2101 gctccgtgac gacaagcacc aggttcgcgc cggtgatcga ggcgatcacc gggcagccga
     2161 tgccgggtga cccgtccacg aggaccgtct ccagcccgcg ctgccgggcg atccggcgcg
     2221 cctcggtgcg aacgatgttg accagcttgc ctgagttctc ctcgcccgcc cccaggcggg
     2281 cgtgcaccat cggcccgcag cgggtctcgg agacgaacca ctcgccgtta tccgcggttg
     2341 cgaactcgat
//
LOCUS       DAOPBQ010000554         2339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_35908_length_2339_cov_3.765762, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000554 DAOPBQ010000000
VERSION     DAOPBQ010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35908_length_2339_cov_3.765762"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1436
                     /locus_tag="PKW60_14055"
     CDS             204..1436
                     /locus_tag="PKW60_14055"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HPK00605.1"
                     /translation="MKFLCLIGDGMADFPLPERGDRTPLQLASIPAMDALAAEGLCGL
                     YWPIPEDLPPGSDIGNLSLFGYNPHETFTGRAPLEAANQGIALGPGDVAFRCNLVTLQ
                     GGAMKSFTSDHISTEEAAELIRFLDAACPRACKLYPGVSYRHLAVIPDSEVKPDALDH
                     TACTPPHDITGQAFEDFLPNGPAGDYLRDLMAWSRPLLADHPINAARIAAGKLPATSI
                     WLWGQGRPPRMASYRDRFGITGAVISAVDLVKGIGVCAGLDVIQVPGATGYIDTNYEG
                     KVEAALRALETVDFVYLHVEAPDECAHEGDIDKKIRAIEDFDKRVVAPCLAAARSHAG
                     WRVLVAPDHVTALGTGTHAGGPVPFALWGCDVATNGVPQYDEASAAAGGLVVNPGHLL
                     VPGLLRPEPLNAHTLMKA"
     gene            1486..>2339
                     /locus_tag="PKW60_14060"
     CDS             1486..>2339
                     /locus_tag="PKW60_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HPK00606.1"
                     /translation="MAQRVEVYDVTLRDGAQGPGVKFSSEDQIRIVKELDAFGVSYIE
                     GGQPGSNPKAAELFQRAKDLDLKHAVMAAFGSTRHARYAVEDDPNIKALLAADTPVVT
                     IFAKFSPTHATQILRVSLEENLRLIAESVAYLKAQDRRVVLDAEHFFDGYREDSEYAI
                     SALECGWQAGAEVLALCDTNGGSLPWDVAEIVQTVRKRLPEAAFGIHTHNDSGCGVAN
                     TLAAVHAGAVHVQGTINGYGERTGNANLCTVIPDLQLKMGREVVSEEQLAQLTRVSHL
                     IAELANMAP"
BASE COUNT          433 a          713 c          729 g          464 t
ORIGIN      
        1 cacaaaaact cgaggagacc cagaagctct tcgaggctgc gcgtgcgcgc tggcttctgt
       61 atttctattt tttctcattt tgcttatttt ggcagtttgg ccgaacctct gtagtttcaa
      121 ggggatgtgt gtttgacaat gccctgtctg tatggtattc ttccgtgttc attcccgggc
      181 gataaccgtg tggagactta cccgtgaagt ttttgtgcct gatcggggac ggcatggccg
      241 attttcccct tcctgagcgc ggcgatcgga caccgctgca gctggcgtcc atcccggcga
      301 tggatgcgct ggcggccgag ggactctgtg gcctgtactg gcctatccct gaagatctgc
      361 cacccggcag cgacattggc aacctttccc tgttcggcta taaccctcac gagactttca
      421 cgggccgcgc ccctctggaa gcggcgaacc aggggatcgc gcttggcccg ggcgacgtgg
      481 ctttccgctg caatctggtg acgcttcagg gcggcgccat gaaaagcttc acgtctgacc
      541 acatctctac cgaggaagcc gccgaactca tccgctttct ggacgccgcc tgcccgcgtg
      601 cctgcaaact ctatcccggc gtcagctaca gacatcttgc cgtaatcccg gactccgaag
      661 tcaagcccga tgccctggac cacacggcct gcactccacc ccacgatata accggccagg
      721 cttttgaaga ctttctgccg aacggtccgg cgggcgatta cctccgcgat cttatggcgt
      781 ggtcgcgccc gcttctggca gatcatccca taaatgcggc ccgcattgct gcgggcaagc
      841 tccctgcgac gtcgatctgg ctgtggggcc agggccgccc gccccgcatg gcatcctacc
      901 gcgaccgttt cggcatcacg ggagcggtca tctcggccgt cgacctcgtc aaagggattg
      961 gcgtgtgcgc cggtctcgac gtgatccagg taccgggcgc cacaggatat atcgacacga
     1021 attacgaagg aaaggtcgag gcggccctgc gcgcgctgga aacagtcgat ttcgtctatc
     1081 tccacgtcga ggcgcccgac gaatgcgcgc acgaaggcga cattgacaag aaaatccggg
     1141 cgatcgaaga ttttgataag cgcgtggtgg ccccgtgtct cgcggccgcc cggtcgcacg
     1201 ctggctggcg cgtgctggtc gcgcctgatc acgtgacggc cctcgggacc ggcacgcatg
     1261 cgggcgggcc ggtacctttc gcgctgtggg gctgcgatgt cgccacgaac ggcgtcccgc
     1321 aatacgacga agcctcagcg gccgctggcg gtctggtcgt taatccaggc catctgctgg
     1381 ttcccggtct cctgcgaccg gagccgctga atgcgcatac tttaatgaaa gcgtaagcgg
     1441 cgcgcttttt gacgcgccct tccgcaggac agggagaacg atagcgtggc acaacgagtc
     1501 gaggtgtatg atgtaaccct tcgcgacggc gcgcagggtc ccggcgtaaa attttcttcc
     1561 gaagaccaga ttcggatcgt gaaagagctg gatgcctttg gtgtgtccta cattgaaggc
     1621 gggcagccgg gttccaatcc gaaggccgcg gagctctttc agcgggcgaa agacctggat
     1681 ttgaagcacg cggtgatggc ggcgttcgga agcacgcgcc acgcccgcta tgcggtcgag
     1741 gacgatccca acatcaaggc cttgctcgcg gcggacacgc cggttgtgac gatctttgca
     1801 aaattctcac caacccacgc cacgcagata ctccgggtca gccttgagga aaacctgcgc
     1861 ctgatcgcag aatccgtggc gtacctgaag gcgcaagacc gccgggtcgt gctggatgcg
     1921 gagcattttt ttgacggata ccgtgaagac agcgagtacg cgatctcggc actggaatgc
     1981 ggctggcagg ccggcgcgga agttctcgcg ctgtgcgata ccaacggcgg cagcctgccc
     2041 tgggatgtcg ccgagattgt acagaccgtt cggaaacgtc tgccggaagc cgctttcggg
     2101 attcacacgc acaacgacag cgggtgcggg gtcgcaaaca cgctcgcggc cgtgcacgcg
     2161 ggggcggtgc atgtgcaggg gacgatcaac gggtatggcg aacggaccgg caatgcgaat
     2221 ctctgcacgg ttatccccga tctacagctc aagatgggcc gtgaggtggt gtcagaagag
     2281 cagctcgccc agttgacacg ggtgtcgcac ctgattgcgg aactggccaa catggcccc
//
LOCUS       DAOPBQ010000555         2323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_36249_length_2323_cov_4.178571, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000555 DAOPBQ010000000
VERSION     DAOPBQ010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36249_length_2323_cov_4.178571"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKW60_14065"
     CDS             complement(<1..208)
                     /locus_tag="PKW60_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00607.1"
                     /translation="MTNKLHRRWIQCLAVAILLAALPAAAQDVPNMLVVVDTADTPAY
                     WNSYVSDINKAYSNVQTAIDDAVTR"
     gene            complement(270..>2323)
                     /locus_tag="PKW60_14070"
     CDS             complement(270..>2323)
                     /locus_tag="PKW60_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00608.1"
                     /translation="ANRWTATDGAYDDWAPSFGPWKDASTHWLDVAGGPVEYGHAFWG
                     TPLRKNVFERQLNLGPSGGVLDPDPLTPDWDPDFGGWALGWQTVRNRAFSSPQQVLEM
                     PHLMMREELTAGGDYAFALNVVERALVGQPELHAYNMVTNTPNVELNTDAVALADSMT
                     GDSIVLTCAQADFYPLAPSPVEIENNAGSADFHRWGTGTTHLPNAWVPIFLHPLGEAV
                     ALPANDVFLQPYHWLPAINNIPSFPDWRVQLNFLLAQPQNFPPGVAVADLAQRWPLWD
                     RPVTFVSANMPNFDPRMDRNDIYDPVNQPALDARPAEALFVWDADDGLEDGEYDVYVI
                     TGDTELDLLRWGQEIAEMHPNLTNLIQQPGFGQEFMNTLNTRAAYLDDLAVDIEFFTD
                     RGRDVWDETGPSPVAGSDGVRDEGYGDGKVWDDTNGNFFPEPGELLRGVNEESFGMRA
                     GVTPTPEGVIHYGTVKVENNYLALFLRNWAPPGMLNRFSRVVLAPRARSHGRININTA
                     QMSPYIISGARHEYNPLMGIPGVLAGFDAATGQFGFLPDIDNYGPSNAVVIAARNLAA
                     RLVEGRYEWPDGRYYRSTGDLVAYDTDTLGTPEQAAAAPPLVTAASGPGEEAQFLEMI
                     GRYGRMANLISAYSDVFEIHVTAEAGYISNEDMNGDGQRDWRYDFVTTAQKKVRTIYE
                     R"
BASE COUNT          413 a          743 c          734 g          433 t
ORIGIN      
        1 accgggtaac cgcgtcatca atggccgtct gcacattgct ataggctttg ttgatgtcac
       61 tcacatagga gttccaatac gcgggtgtgt ctgccgtatc caccaccacg agcatgttgg
      121 ggacatcctg cgcggcggcg ggcagggcgg cgagcagaat cgccaccgcc aggcattgaa
      181 tccatcggcg atgcaatttg ttcgtcatcg gccactctcc agtatcatgg cgcgcggcat
      241 ggcaaccgcc tgtccgggcc gtgtggtacc taccgctcat agatggttcg cacctttttc
      301 tgggcggtcg tcacaaaatc gtaccgccaa tcccgctggc catcgccgtt catatcttcg
      361 tttgagatat acccggcctc ggccgtcaca tggatctcaa acacatcgga ataggccgag
      421 atgaggttcg ccatccgccc ataccgtccg atcatttcca gaaattgcgc ttcttcgccc
      481 gggccgctgg cggctgtgac cagcggcggc gccgcggcgg cttgttcggg cgtgccgagc
      541 gtgtccgtgt cgtaggccac caggtcgccc gtggagcggt aataacggcc gtcaggccac
      601 tcataccgcc cctcgacgag gcgggcagcc agattccgcg cggctatcac gaccgcattg
      661 ctcggaccgt agttgtcgat gtcgggcagg aagccgaact ggcccgtggc cgcatcgaac
      721 cccgccagca cgccgggaat gcccatcagc gggttgtact cgtgccgcgc gccgctgatg
      781 atatagggcg acatctgggc tgtattgatg ttgatccggc cgtgggagcg tgcacgcggc
      841 gccagcacca cgcgcgagaa ccgattcaac atgcccggag gcgcccagtt ccgcaggaac
      901 agggccaggt agttgttctc caccttcacg gtgccgtagt ggatcacgcc ctcgggagtc
      961 ggcgtaaccc cggcccgcat cccaaacgac tcctcattca caccccggag cagctcgccg
     1021 ggttcaggga agaaattacc gttcgtgtcg tcccagacct tgccgtcgcc gtaaccctcg
     1081 tcgcgcacgc cgtccgagcc ggcaacggga gaaggacctg tctcatccca cacatcccgc
     1141 ccgcgatcgg tgaagaactc gatgtcgacg gcgaggtcgt ccaggtaggc ggccctggtg
     1201 ttcagcgtat tcatgaattc ctgcccgaac cccggctgct ggatgaggtt ggtcaggttg
     1261 ggatgcattt ccgcgatctc ctggccccag cgcagcagat cgagctcggt gtcgccggtt
     1321 atcacgtaaa catcgtattc gccatcctcg aggccgtcgt cggcatccca cacaaacaag
     1381 gcttcggcgg gtcgcgcgtc gagggcaggt tggttgacgg ggtcgtagat gtcattccgg
     1441 tccatgcgcg ggtcgaaatt gggcatgttg gccgagacga aggtcaccgg gcggtcccag
     1501 aggggccagc gttgcgccag atcggccacg gccacgccgg gcgggaaatt ctgcggctga
     1561 gccagaagga agttgagctg cacccgccaa tccgggaagc tgggaatgtt gttgatggcc
     1621 ggcagccagt gatacggctg caggaacaca tcgttcgcgg gcagcgccac cgcttcgcca
     1681 agcggatgca ggaagatcgg cacccacgca ttcggcaggt gggtcgtgcc cgtgccccac
     1741 cggtgaaaat ctgcagaacc cgcattgttc tcgatttcga ccggactggg cgccagcggg
     1801 tagaaatcgg cctgggcgca ggtcagcacg atggaatcgc cggtcatact gtcggccagt
     1861 gccactgcgt cggtgttcag ctccacgttg ggtgtgttgg tcaccatgtt gtaggcatgg
     1921 agttccggct gtccgaccag cgcgcgctcg accacattga gcgcaaacgc gtaatcgcct
     1981 ccggcggtca gctcttcgcg catcatcaga tgcggcatct caagcacctg ctgaggcgac
     2041 gagaaggcgc ggttgcgcac ggtctgccag cccagggccc atccgccgaa atcggggtcc
     2101 cagtcgggtg tcaggggatc aggatccagg acgccgcccg aaggcccgag attgagctgc
     2161 cgttcaaaca cgttcttgcg cagcggcgtg ccccagaacg cgtgcccgta ttcgactggt
     2221 ccgcccgcca cgtccagcca gtgggtcgag gcatctttcc acggcccgaa gctcggcgcc
     2281 cagtcgtcgt acgcgccgtc ggtggcggtc cagcggttcg cat
//
LOCUS       DAOPBQ010000556         2319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_36356_length_2319_cov_3.731890, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000556 DAOPBQ010000000
VERSION     DAOPBQ010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36356_length_2319_cov_3.731890"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PKW60_14075"
     CDS             <1..444
                     /locus_tag="PKW60_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPK00609.1"
                     /translation="RAETAVRSGIRFIQFAVRPEEDAVALDEYIKSVTAMPSPHLANG
                     TLSDAARRGEALFQQTGCAACHPGPLYTDLKPYNVGTGKDRETDMKFDTPTLVEIWRT
                     APYLHDGRAVTMLDMLKSETSGPQHCPSGLTEEQLADLAAYVLSL"
     gene            570..1676
                     /locus_tag="PKW60_14080"
     CDS             570..1676
                     /locus_tag="PKW60_14080"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPK00610.1"
                     /translation="MSLRQESSVQIRTIPRTGYQELFITAAPQPGQDLGELMLEVFQE
                     LGERHASVVSLELFGVSRDAGEAAFRCVAAVHGDPAWPVLWISEGADAEPPAVAVQVW
                     AVTQLEVSPLRSNDRVIGIRYEDESAEYCRLSGIIPGDLALAEGEQALSVFEQMEAGL
                     AAAGMDFHDVLRTWFYNDRITAWYSEFNKARDTMFKRWRVFDGLVPASTAVGGANAFG
                     AALTGGLIAVKAKTDGVLRQPLPSPLQCPALDYGSSFSRAVEFETPDHRRIYVSGSAS
                     IAPEGHTIFIDDVEGQIARTMEVIGAILESREMDWKDVCRAIAYFKDPANIGLFSKYC
                     ESQGIFDLPVAHVKDDICRDDLLFEVELDAIVAK"
     gene            1735..>2319
                     /locus_tag="PKW60_14085"
     CDS             1735..>2319
                     /locus_tag="PKW60_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00611.1"
                     /translation="MLKHTALCLSIAMLASGIALAAEDARPIPWRLEPVKTIKGCKVP
                     ECVVVDAENALAYISNVEALPDEYWSDDGRGFISLMTVDGKMLKRRWLNSAPGAPIHG
                     PKGMCILNDVLYIADNSRLLARPLQPGGQLEVLSVPGAQRINDLATEGTHVFASDTTR
                     EVIYKVNPVSGEVVILKSPPSVNGITFHLGAFFAV"
BASE COUNT          445 a          709 c          730 g          435 t
ORIGIN      
        1 cgggccgaga ctgcggtccg gtcgggtatc cggttcatcc agtttgcggt gcggccggag
       61 gaagatgcgg ttgcccttga cgaatacata aagagcgtga cggcgatgcc cagcccgcat
      121 ctggcgaacg gcacgctgag cgatgccgcc cggcggggtg aagccctgtt ccagcagacg
      181 ggttgtgccg cctgccatcc cgggccgctc tacaccgacc ttaaaccata caacgtcggg
      241 accggcaagg accgcgaaac ggatatgaag ttcgacacac cgacccttgt cgagatctgg
      301 cgtaccgccc cgtatctgca cgacggccgg gctgtcacca tgctcgatat gctgaagagc
      361 gaaacatccg gaccgcaaca ctgtccctcc ggtctgacgg aggagcagct tgccgatctc
      421 gccgcctatg tcctgtcgct ataacgcccg atggaagtca acgccaggga agtccggcgc
      481 cgggttttgc cccgcgccga atagcttaac ccggtctcac gggtacgtgt tcacgtgctg
      541 aatgtgagac gggcatgaag gataaaaacg tgagtttgag acaagaaagc agcgtgcaga
      601 tccgcaccat cccgcgaacc ggctaccagg aactgttcat tacggcggcg ccccagcccg
      661 gccaggattt gggcgagttg atgctcgagg tgttccagga gcttggcgaa cgccacgcct
      721 cggtggtgtc cctggaactg ttcggagttt cccgcgacgc cggtgaggca gcgttccgct
      781 gcgtggcagc cgtgcacggc gacccggcct ggccggtgct ctggatatcg gaaggagcgg
      841 atgccgaacc gccagcagtt gccgtccagg tctgggccgt gactcagctc gaggtcagtc
      901 ctctgcgaag caacgaccgg gtcatcggca ttcgctacga ggacgagagc gctgaatact
      961 gccggctcag cggaatcatt cccggcgacc tggccctggc cgagggcgaa caggcgttgt
     1021 ccgttttcga gcaaatggag gccggcctgg cggctgccgg catggatttt cacgatgtgc
     1081 tgcgcacctg gttctataac gaccgtatca cggcatggta ttcggaattc aataaggcgc
     1141 gcgacaccat gttcaagcgc tggcgcgtgt ttgacggact ggtccccgca agcacggccg
     1201 tgggcggcgc gaatgcgttc ggcgccgccc tgaccggcgg tttgatcgcg gtcaaggcca
     1261 agacagacgg agtattgcgg cagccgttgc cttcgccgct gcaatgccct gcgttggatt
     1321 atggcagctc attcagccgc gcggtggagt tcgagacacc ggatcaccgc cgcatctacg
     1381 tttccgggtc cgccagcatc gccccggaag gtcacacgat cttcattgac gacgtcgaag
     1441 gacagatcgc ccgcaccatg gaggtgatcg gcgccattct cgaatcccgc gagatggact
     1501 ggaaggacgt ttgccgcgcc atcgcctatt tcaaagatcc ggctaacatc gggctcttct
     1561 cgaaatactg cgaatcccag ggcatcttcg atctcccggt ggcacacgta aaagacgaca
     1621 tctgccgcga tgatcttctt tttgaggttg aactcgacgc gattgtggca aaataggcgg
     1681 cgcatcatgg caacgcaggt gaaacatgcc gccgcttggg ctggaggttg tgtcatgctg
     1741 aaacatacgg cgctgtgcct gagcatcgcg atgctggcct ccggtatcgc gctggccgcc
     1801 gaggacgcca ggccgatccc gtggcggctg gagcccgtaa agaccatcaa aggctgtaaa
     1861 gtgcctgaat gcgtggtggt cgacgccgag aatgccctgg cctacatttc caacgtcgaa
     1921 gcgcttcccg acgagtactg gtccgacgat gggcgggggt tcatttccct catgacggtc
     1981 gacggcaaga tgctcaagcg gcgctggctc aacagcgcgc ccggcgcgcc catccatggg
     2041 cccaagggga tgtgcatcct caacgacgtg ctctacattg ccgacaacag ccgcctgctg
     2101 gcccggccgc tgcagccggg cggccagttg gaagtgcttt ccgtgcccgg tgctcagcgg
     2161 atcaatgacc tggccaccga gggaacgcat gtctttgcca gcgacaccac gcgcgaggtc
     2221 atctacaagg tgaatcccgt gtccggagag gtcgtcattc tgaaatcacc cccatccgtc
     2281 aatgggatca cgtttcacct gggcgcgttt ttcgcggtg
//
LOCUS       DAOPBQ010000557         2318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_36380_length_2318_cov_3.733540, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000557 DAOPBQ010000000
VERSION     DAOPBQ010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36380_length_2318_cov_3.733540"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PKW60_14090"
     CDS             complement(<1..351)
                     /locus_tag="PKW60_14090"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00612.1"
                     /translation="MRHSTRRDFLKAAGTAAAATAWSARSYSQIQGANDRMGVAIIGC
                     GNMGGAHLRTLLGQRDSENIELRAVCDVWQTRANRFRDLAREAGADPVAAQDYRDVLA
                     MKDVDYVVLPLPNHW"
     gene            617..1228
                     /locus_tag="PKW60_14095"
     CDS             617..1228
                     /locus_tag="PKW60_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HPK00613.1"
                     /translation="MDTTEDHYMNGVVTIQEADALLVVDVQKDFCPRGALAVENGDAV
                     VPVLNALVPKFGCVVFTRDWHPADHCSFAETPRFEDKSWPAHCVANTPGAAFHDNLNV
                     PSHALIIDKATTPEAEAYSGFEGTDLAEQLKERGIARVFVGGLATDYCVKNTALDAVR
                     HGFDAVVVLDACRGIDIPSGTVEAALAEMRQRGVQFVLAEGIR"
     gene            1228..>2318
                     /locus_tag="PKW60_14100"
     CDS             1228..>2318
                     /locus_tag="PKW60_14100"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPK00614.1"
                     /translation="MPATPWCQREGMALLTDLYELTMVAGFWKEQRANQRVCFEYFFR
                     RLPPHAGFAVAAGLEPFLQYLENLHFSSDDLAYLRTLNLFDDAFLAYLRDFRPSLTVR
                     AVPEGTLVFPYEPILQVEGSVLEAQFVETALLNTLNYQTLIATKAARICLAAEGEPVM
                     EFGLRRAHGPDGGLSGSRAAYIGGCSSTSNLLAGKVYGIPVSGTHAHSWVMSFPSELE
                     AFRAFARNYPDRCMLLVDTYDTLGSGVPNAITVFKELHAKGQMARPAIRLDSGDLAKL
                     SKMAYRMMTEAGLKDPLIVASNDLDEDLIADLKRQGARINAWGVGTHLITSNDCPALS
                     GVYKLVAVAQGEAWRPKIKISSNIEKATDP"
BASE COUNT          436 a          693 c          733 g          456 t
ORIGIN      
        1 ccagtggttc ggcagcggaa gcaccacata atccacatct ttcatggcca gcacgtcgcg
       61 gtaatcctgg gccgccacgg ggtccgcgcc ggcctcgcgg gccaggtcgc ggaaccgatt
      121 cgcccgggtt tgccacacat cgcacaccgc gcgaagctcg atattttcgg agtcgcgctg
      181 tcccagcaag gtgcgcagat gggcgccgcc catattgccg cacccgatga tcgccacccc
      241 catgcggtcg ttggcgccct ggatctggct gtagctccgg gcactccacg ccgtagccgc
      301 cgcggcagtc cccgcggctt tcaggaaatc ccgccgagtc gaatgtctca tgacctgcct
      361 gcctttcccg tgcctgcctg tctgtcgttc cgagcatata gcccgctgcc gtttcgtgca
      421 agagcattcg atgcagggct cgagctgtca tccggcaatt tgaaagcggc ggggcagggc
      481 cttgctcctg taaagggcat gccggcacgc cagtcgagcc cgacaatttg cgcatgaacc
      541 acgatacatg ggcatacgtt actttcttta tacggtgcag gtgtggttaa atcgtaggca
      601 ctacgattct ggcgcaatgg acacaactga ggaccattac atgaacggcg tggtaacaat
      661 acaggaggcg gacgccctgc tggtggtgga tgtccagaaa gacttctgcc cgcgcggcgc
      721 gctcgcggtt gaaaacggcg atgccgtggt gcccgttctg aacgcactgg tgccgaaatt
      781 tggctgcgtg gtgtttacgc gcgactggca ccccgccgat cactgcagtt ttgccgagac
      841 gccccggttc gaggacaaaa gctggcccgc ccattgcgtg gcaaacacgc cgggcgcggc
      901 gtttcacgat aacctgaacg tccccagcca tgccctgatc atcgacaagg caaccacacc
      961 cgaagccgaa gcctacagcg gatttgaagg cacggatctg gcggaacagc tgaaagagcg
     1021 cggcatcgcc cgggtgtttg tcggcggcct ggcgacggat tattgcgtaa agaacacagc
     1081 cctggatgcg gtgcggcatg ggtttgatgc ggtggtggtg ctggacgcct gccgcggcat
     1141 tgatattcca tccgggactg tggaggcagc tctcgctgag atgcggcagc ggggagtcca
     1201 gtttgtcctt gcggagggta tccggtaatg ccagcaacac cctggtgcca gcgcgaagga
     1261 atggcccttc tgacggattt gtatgaactg accatggtcg ccgggttttg gaaagagcag
     1321 cgcgcgaacc agcgcgtgtg cttcgagtat tttttccggc ggcttccgcc tcatgcaggt
     1381 ttcgccgtcg cggcaggatt ggagccgttt ttacagtatc tcgaaaacct gcatttcagc
     1441 agcgacgacc tggcgtatct gcgtacgctg aacctgttcg acgacgcctt cctggcgtac
     1501 ctgcgggatt ttcggccgtc actgacggtc cgggcggtgc cggagggcac cctggtcttt
     1561 ccctatgagc ccattctgca ggtggaaggc tccgtgctag aagcacaatt tgtcgaaacg
     1621 gcgcttctga acacgctgaa ttaccagacc ctgattgcca cgaaagccgc tcgcatctgc
     1681 cttgccgcgg agggcgaacc ggtgatggaa tttggcctgc gccgggcaca tgggccggac
     1741 gggggactga gcggctcgcg cgcggcctac atcggaggat gctcgtctac gtcgaacctg
     1801 ctggcgggca aagtctacgg cattcccgtg tcgggtacgc acgcgcacag ctgggtcatg
     1861 agttttccca gcgaactcga ggcctttcgg gcattcgcga ggaattatcc cgaccgttgc
     1921 atgctgctgg tggatacgta tgacaccctg ggcagcggcg tgccgaacgc gatcaccgta
     1981 ttcaaggagc tgcatgcgaa ggggcagatg gcccggccgg cgatccggct ggattcaggc
     2041 gacctggcca agctcagcaa gatggcctac cgcatgatga cggaggccgg tttgaaggat
     2101 cccctgatcg tcgcttccaa cgacctggat gaggatttga tcgcggacct caagcggcag
     2161 ggcgcccgga tcaacgcgtg gggcgtcggc acccatctca tcacatcaaa cgattgcccg
     2221 gccctcagcg gcgtgtataa gctggtggcc gtggctcagg gtgaggcctg gcggccgaaa
     2281 atcaagatct cgtccaatat cgaaaaggcg acggatcc
//
LOCUS       DAOPBQ010000558         2300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_36753_length_2300_cov_3.189755, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000558 DAOPBQ010000000
VERSION     DAOPBQ010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36753_length_2300_cov_3.189755"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKW60_14105"
     CDS             complement(<1..443)
                     /locus_tag="PKW60_14105"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK00615.1"
                     /translation="MKKDFINRFLLVFVTVGVFLHIPSAHGQPGEHTAPEWNALGVEY
                     YEAGDWNRALHCFLEAASLVPGNETVQRNLANAYQARAAELADGGDYSSAAQHLEEAI
                     KASPESPLPRVQLGSYFLRMSLVQDAIVRLEEAILLAPENLDAHEL"
     gene            complement(453..839)
                     /locus_tag="PKW60_14110"
     CDS             complement(453..839)
                     /locus_tag="PKW60_14110"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPK00616.1"
                     /translation="MEKRCIHSKNGPPAQGPYSHAVAAGRLLFVSGQIAVARDGSGMQ
                     KSNLEDETRLALTNLKTILEDAGSGLQHVVKVTVYLSNMRNFSEFNEIYKEYFPMNPP
                     ARTCIEVGKLPLDALVEVDAIAIIPN"
     gene            complement(952..2205)
                     /locus_tag="PKW60_14115"
     CDS             complement(952..2205)
                     /locus_tag="PKW60_14115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK00617.1"
                     /translation="MFKPLYRQFAAAFLFDFAMVCGLTASPFYIFNVLEGGAGMSGVI
                     TGLQWALYAVASIVSARYVGRLSNGLLMGVLGATGFAVLFPLGVLISNPYFYVALTTA
                     GMIFPALFWPAMQSWVGAEPDPAVRTHRLALYNLSWTLGLTCAPFATWLLSPIDYRLP
                     FLATALCAGGAAALAASLPHESRMAAPDAETTRTANRGHTTRSEAHLLSAWLSLVLGS
                     TLFNAVTAVFSFRMEELVKAGALCLFHAGTSAPLTAEPVVYFSILAMIMNGMRAGTSL
                     VMGHSTVWQHRFWVLALFQAAAAVAFWVLSFTMSLAVMAVCCAVVGIAAGVCFFASQS
                     YSVANSVKKHQRVSIHEGMVGLGSLLGAVGFGLLADWRGTPWPFANTPFLIAGGLALE
                     AALLRIGFLHFRKNTAPESQPEHAS"
BASE COUNT          516 a          655 c          707 g          422 t
ORIGIN      
        1 agctcgtgcg catccaggtt ttccggagcg aggagaatcg cctcttcaag ccggacaatg
       61 gcatcctgga caagagacat gcgaagaaag tacgacccca gctgaacccg ggggagagga
      121 ctctccggag aggctttgat ggcttcttcg agatgctggg ccgcgctgct gtagtcgccc
      181 ccatctgcca gttccgcagc gcgtgcctgg tacgcattcg ccaggttacg ctgaacggtt
      241 tcattaccgg gtacgagtga ggccgcctcg agaaagcagt ggagggcgcg gttccagtcg
      301 ccggcctcgt aatattcgac accaagcgca ttccattccg gagccgtgtg ctcgcctggc
      361 tgcccgtggg ctgacgggat gtgcaggaac acaccaaccg tcacaaatac caggaggaat
      421 ctgttgataa agtctttttt catgccgcgt cttcagttcg gaatgatcgc gatagcgtcc
      481 acttcaacca gggcatcgag cgggagcttt cccacctcga tgcaggttcg ggccggagga
      541 ttcattggga aatattcttt ataaatttcg ttgaattcgc tgaaattgcg catattggag
      601 agatatacag tgaccttaac tacgtgctgc aggcctgagc cggcatcttc aagaatggtc
      661 ttgaggttcg tcagcgccag gcgcgtttca tcctccagat tgcttttctg catgccgctg
      721 ccgtcgcgtg caacggcaat ctgcccggac acaaacagca gccgtcccgc agcaacagca
      781 tgcgaatacg ggccctgggc agggggccca ttttttgagt ggatgcatcg cttttccatg
      841 gaaatcgctc cgtgcgcatc tgttgtccag taaagaaact accacacgct gtcgggagcg
      901 tcaaatgtcg ttcgatacgc gtttttctgc cgctccggca ggcaacccgg gtcaggaggc
      961 gtgctcaggc tggctctcgg gcgctgtgtt tttccggaaa tgcagaaagc cgatccgcag
     1021 cagtgcagcc tcgagcgcca gccctccggc aatcaaaaac ggcgtgtttg cgaaaggcca
     1081 gggcgtgccg cgccagtcgg cgagcagtcc gaacccgacc gcacccagaa gcgagcccag
     1141 gccgaccatg ccttcgtgaa tggacacccg ctggtgtttc ttgacagaat tggccacgct
     1201 gtatgactgg ctcgcgaaaa agcatacccc cgcggcgatg cccacaacgg cacagcaaac
     1261 ggccatgacc gccaggctca tggtaaacga caagacccag aatgccactg cggctgcggc
     1321 ctggaagagc gccaggaccc agaaccggtg ctgccagaca gtgctgtgcc ccatgacaag
     1381 ggaggtgccc gcgcgcatgc cgttcattat catggccagg attgagaaat acacgacagg
     1441 ttcggcggtc aggggagcgc tcgttccggc gtgaaagagg cataaagccc cggctttgac
     1501 gagctcttcc atacggaagc tgaaaacggc cgtcacggcg ttaaagaggg tgctgcccag
     1561 caccagggag agccaggccg acaggagatg agcttcgctg cgtgtggtgt ggccgcggtt
     1621 ggcagtacgg gtcgtttcgg catccggcgc ggccatgcgc gattcgtggg ggaggctggc
     1681 ggcaagcgct gctgccccac ccgcacacag ggccgtggcc agaaacggca gccggtagtc
     1741 gatgggcgag agaagccagg ttgcaaacgg cgcgcaggtg agcccgaggg tccaggaaag
     1801 gttgtacagg gcgaggcggt gcgttcgcac cgcaggatcg ggttcggcgc ccacccacga
     1861 ctgcatggcc ggccagaaca gggctggaaa tatcatcccc gccgtagtca aggcaacata
     1921 gaagtatggg ttagaaatca acacaccgag cgggaagagg acggcgaatc ccgtggcgcc
     1981 caggacgccc atcaagagtc cattggaaag gcggcccaca taacgcgcgg aaacaatgct
     2041 cgcgacagca tacaaagccc attgcaggcc cgtgatgacg ccggacatgc ccgcgccccc
     2101 ttccaggaca ttaaaaatgt agaagggcga ggcggtcaat ccgcagacca tcgcgaaatc
     2161 gaatagaaaa gcagcggcaa attgtcgata cagtggtttg aacatggtcg cgcgagtcta
     2221 cggtggcgcc atcaaaatat caaggcggag ctatctcaag caggactttt cccaagattt
     2281 tgtttgattt gcgtcagttg
//
LOCUS       DAOPBQ010000559         2298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_36788_length_2298_cov_2.943379, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000559 DAOPBQ010000000
VERSION     DAOPBQ010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36788_length_2298_cov_2.943379"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /gene="queF"
                     /locus_tag="PKW60_14120"
     CDS             complement(<1..341)
                     /gene="queF"
                     /locus_tag="PKW60_14120"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HPK00618.1"
                     /translation="MENRPAKEQFKALDKTLPFKGPEAIESGLLECFPYEFAGKPIGN
                     SLEIVTETAEFTSVCPFSGLPDFARITITYTPGELCLELRSLKYYLLSYRDVGIWYEH
                     LVNRMLEDLVAA"
     gene            complement(331..681)
                     /locus_tag="PKW60_14125"
     CDS             complement(331..681)
                     /locus_tag="PKW60_14125"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPK00619.1"
                     /translation="MIPPCPGTDQPGAIRFTFEAAQSLAQLPDTHKCRRMHGHSYRVE
                     VAANDPEVLREPLRDVYELLDHTCLNDVPGLEIATCEVICHWLWQRLAACPAGIRAVA
                     VQETPSARCTYYGE"
     gene            complement(715..1029)
                     /locus_tag="PKW60_14130"
                     /pseudo
     CDS             complement(715..1029)
                     /locus_tag="PKW60_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
     gene            complement(1030..1743)
                     /locus_tag="PKW60_14135"
     CDS             complement(1030..1743)
                     /locus_tag="PKW60_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK00620.1"
                     /translation="MSSPPLPPDDASKHPARPAKASEKTLVVTEIYRSIQGESTWAGR
                     PCTFIRLTGCNLRCVWCDTSYAFKGGTRMTIAEILAECRRLGCALVEITGGEPLLQKA
                     CPRLAEALLAEGYTVLCETSGSLPIADLPRDVVKIMDLKCPGSGECEKNDWANLDALG
                     PRDEIKFVIASRADYEWSRDVFRKYNLAARCHAVLFSPVFGAIAPKDIVAWLLEDALD
                     ARFQLQLHKFIWPPSQKGV"
     gene            complement(1978..>2298)
                     /locus_tag="PKW60_14140"
     CDS             complement(1978..>2298)
                     /locus_tag="PKW60_14140"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPK00621.1"
                     /translation="RRMPAMTLDTVYRTLGTFEAMGLIERVAAVGNSARFEANLAPHH
                     HFVCKRCGSILDVPPGRFEPSEFTGDLPDGSLLHSARIDMRGICSQCARSSASHRKPA
                     RLIE"
BASE COUNT          435 a          643 c          774 g          446 t
ORIGIN      
        1 gcggcgacca ggtcttcgag catacggtta acgaggtgtt cgtaccagat cccgacgtcg
       61 cggtacgaaa gcaggtagta tttgagcgaa cggagttcga ggcacagctc gccgggagtg
      121 taggtgatgg tgatgcgagc aaaatcgggc aatccggaaa aggggcaaac ggaggtaaat
      181 tcggcggttt cggtgacgat ttcaaggctg ttgccgatgg gttttccggc gaattcgtaa
      241 ggaaaacact cgagaaggcc ggattcgatg gcctcgggcc ctttgaacgg gagggtcttg
      301 tcaagggctt tgaattgctc ctttgcgggc ctattctcca tagtacgtgc acctcgcgga
      361 aggggtttcc tggacggcga ctgcgcggat gccggcgggg caggccgcga gccgttgcca
      421 gagccaatgg cagatcactt cacaggtggc gatttcgagc ccgggcacat cgttcaggca
      481 cgtgtgatcc aggagttcgt agacgtcgcg cagtggttcg cgcaacacct cggggtcgtt
      541 ggccgcgact tccacacggt agctatggcc gtgcatgcgg cggcatttgt gggtgtcggg
      601 cagctgagcc agggattgtg cggcctcaaa cgtgaagcgg atggcgccgg gctgatccgt
      661 gccggggcac ggcgggatca cctcgggggc atacgtacag tcgccgacaa tagcgacccg
      721 gacgtccttc aggccgggca gcgcgggcgc cagtttgcgc agaatccaat cgcgcagccc
      781 gggtacggtc ggctcatcga gatccgggat gtcgttcagg agcgcatgat ctatgtgctg
      841 atagattggc ttgaatgccg ccgtgatatc gccgtagtca atcagccagc cgaactctga
      901 ggtgacgtcg ccttcgacca ccagcacgat ctcgtagctg tgtccgtgca gacggcgggt
      961 tttgggtggt ccgcccgggt tccggtgggc cgcttcggcg aagaacgttt tggtgagttg
     1021 aaccttcaat cagactcctt tctggctggg gggccagatg aatttgtgca gttgcagttg
     1081 aaaccgggca tcgagggcat cttccaggag ccacgccaca atatctttcg gcgcgatggc
     1141 gccgaagacg ggggaaaaca ggactgcgtg gcagcgtgcg gccagattgt acttcctgaa
     1201 gacgtcgcgg ctccattcat aatcggcgcg gctggcaatg acaaacttga tctcatcgcg
     1261 gggaccgagc gcgtcgaggt tggcccagtc atttttctcg cattcgccgc tgccggggca
     1321 tttgaggtcc atgattttaa ccacgtcgcg ggggaggtcg gcaatcggaa gggagccgct
     1381 ggtttcgcac aggacggtgt atccttcggc cagcagggcc tctgccaggc gcgggcatgc
     1441 cttttgcagc aggggttcgc cgccggtgat ctcgaccagt gcacagccga ggcggcggca
     1501 ctccgcgagg atctccgcga tcgtcatccg ggtaccgcct ttgaacgcgt agctggtatc
     1561 gcaccagacg cagcgcaggt tgcagccggt gagccggata aaggtgcatg gccggccggc
     1621 ccaggtcgat tcgccctgga tgctccggta gatctcggtc acgacgagcg tcttctcgga
     1681 cgcttttgcc ggacgcgcag gatgcttcga ggcatcgtct ggcggcaacg ggggtgagga
     1741 catacacatt cctgaagtta cgcagtgtca cgcgcttcaa tcctgtccgc gggcgcgcac
     1801 accgacggga accattataa gaaacccgga cagcgggaga aaacacaaga ccgccagcgg
     1861 tctcgaggtt tttcgctggc cggacggcat gccgggcctc aggacgatgc cgggcggata
     1921 ccccacggtt ttgtgctttc cagaggcggc gcaaaactcc gcgaagcagg ccgcgctcta
     1981 ttctatgagg cgggctggct ttcgatgcga tgcagacgat cgcgcgcact gggagcagat
     2041 tccccgcatg tcgatccggg ctgaatggag caggctgccg tccggcagat cgcccgtgaa
     2101 ttcggacggc tcgaagcggc ctggaggcac atcgagaata ctgccgcagc gcttgcagac
     2161 aaaatgatga tgcggcgcca gattggcctc gaaacgcgcg ctgttcccaa ctgcagcgac
     2221 gcgttcaatc agccccatcg cttcaaaggt tcccagcgtg cgatataccg tgtcgagcgt
     2281 catggccggc atgcggcg
//
LOCUS       DAOPBQ010000560         2296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_36832_length_2296_cov_3.840250, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000560 DAOPBQ010000000
VERSION     DAOPBQ010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36832_length_2296_cov_3.840250"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1744
                     /locus_tag="PKW60_14145"
     CDS             260..1744
                     /locus_tag="PKW60_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00622.1"
                     /translation="MPLASARLYLDIFRPPVYETCDPEAWLKAYAGALEDDRCSLHLQ
                     AVSSCVCDPAQAWACLSTLTSGVELHEDAVLLVELFEEEDIPRIALTLDGPGTFPARF
                     RVGGDLPIPFETLCERWTVATRGGRIRRTPAGLDLRLRGLREPETSDESLAPFVDCVS
                     KLNRSIMECLAAQDGGPKDAVPWPSALGQAEDCLALLDGSMPTGSADFVRTIRDAVAA
                     EADVTSEQALEVDVFAERELPPLMITRPALFRAWESTVQHALMSLPRGGRLAFMVDYD
                     ANARSVSTLIELEGTQYIPGETVYLASIRRGVVELHEGTVECSAELNGMLITVTIPDR
                     VGKTLDSWLPGWERFSEQSRQMLRLLKSGDSPLPEEFLLGGILENELERWLLPLLTEP
                     VMVNLAHDGVESLDGLPGASKERLDKALSQIRRGKPKKEIARPPYAGELLWAFRAPLH
                     ARNALRLNTLDDGELRELCLELLQPSVDSARCLRFIAHAAVPEL"
     gene            2048..>2296
                     /locus_tag="PKW60_14150"
     CDS             2048..>2296
                     /locus_tag="PKW60_14150"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPK00623.1"
                     /translation="MGFIDQVIERAQANPRTIVLPEADDERVLKAAGEIQARKIASVT
                     LIGAVDTVTRKLNELGIKGEFEIVDPARAPWADEFANDF"
BASE COUNT          423 a          653 c          752 g          468 t
ORIGIN      
        1 ttgtgctgta tttctttccg tagatttcca tggtgactcc ctccgcatgg aatgtctgtt
       61 acgctggcgt gtactctaag gctttgagag gcgccatgca aaaacgtgct ggaatggcgc
      121 cggcggcata cgtacactgg gccccgttat gaatgcgatt gcggctttga tagaaccggc
      181 tgtggcgtgc gtccgcgaaa ggcttgccac acgatgcgcg gatctttccg atacgccaac
      241 ggattgcgcg gacctcctgg tgccgttggc atcggcccgg ctgtatctgg acatctttcg
      301 tccgccggta tacgagacct gcgacccgga agcatggctg aaagcatacg cgggcgccct
      361 ggaagatgac cgctgctctc tgcatctcca ggccgtctct tcctgcgttt gcgatccggc
      421 gcaggcgtgg gcatgcctgt caaccctgac gtccggcgtt gagttgcacg aagacgccgt
      481 gcttctcgtg gagctgttcg aagaagagga cattccgcga atcgccctga ccctggatgg
      541 cccggggacg tttcccgcgc gtttccgggt tgggggggac ctgccgatcc cctttgaaac
      601 gctttgcgaa cggtggacgg ttgcgacccg cggaggccgg attcggcgga caccggcggg
      661 gttggatctg cgtctgcggg gactgcgtga accggagacc tcggatgaga gcctggcgcc
      721 gtttgtcgat tgtgtgtcca aactgaatcg ttcgatcatg gaatgtctgg ccgctcagga
      781 cggtgggccg aaagacgcgg tgccctggcc cagcgccttg gggcaagccg aggactgcct
      841 cgccctgctc gatggttcca tgcccacggg ctccgctgat tttgtgcgga caatccgcga
      901 cgcggtcgcc gcggaggctg acgtcacgtc agaacaggcc ctggaggtgg acgttttcgc
      961 ggagcgggaa ctccctccgc tgatgatcac gcggccggcg cttttcaggg cctgggagag
     1021 caccgttcaa cacgccctca tgagtctgcc tcgcggagga cggctcgcgt tcatggtgga
     1081 ttacgatgcc aacgctcgca gcgtttcgac cctcatcgaa ctggagggca cccagtacat
     1141 acccggcgag acggtctacc tggcctccat ccggcgggga gtagtggaac tccacgaagg
     1201 gaccgtcgaa tgcagtgcgg aactgaacgg catgctgatc acggtgacga ttcccgatcg
     1261 tgtggggaaa accctcgact cgtggctgcc gggatgggaa cgattctctg agcagtcgcg
     1321 tcagatgctg cgattgctga aaagcggcga ttcgcctctg cccgaggagt tcctgctggg
     1381 cgggatcctc gaaaacgaac tcgagcgatg gcttttgccg cttctgacgg agccggtgat
     1441 ggtcaacctg gctcatgacg gagtggagag cctcgacggc ctgccgggcg catcaaagga
     1501 gcgcctcgac aaggcgctca gccagatcag gcgcggcaag cccaagaagg agatcgcgcg
     1561 cccaccttac gccggggagc tgctgtgggc ctttcgtgct cccctgcacg cccggaacgc
     1621 gttgcgactg aatacgttgg acgacggcga gctgagagag ctctgcctgg aattgctgca
     1681 accgtctgtc gacagcgcac gctgcctgcg cttcatcgca cacgcggccg taccggaact
     1741 gtgaggccgc acaatccccg caaatgaagc agtccgggcg tgcccaggcg cggtttttga
     1801 tgctacgctg gcgctacgtt gacactgctt ttgacattgt tctagactac gctccgtggt
     1861 ccctgtttta cattcagcaa gtcaagactg tgatttgccg gggatgcgca gggttcaagt
     1921 ctcaatggag gtggtcgcct gaagtccacg cacggcggcg cgttcgtgat tatgtcctct
     1981 attcagggaa ccggtttcca gggggtgtgt tgtgttcacg gagctgccag gaaagggtac
     2041 gtaaaatatg gggtttattg accaggtaat cgaacgagct caagccaatc ccaggaccat
     2101 cgtgctgccg gaagccgatg acgagcgcgt attgaaggcg gcgggcgaaa ttcaggcgcg
     2161 aaagatcgcc tccgtgaccc tgatcggcgc agtggacacc gtgacgagga aacttaacga
     2221 actggggatt aagggggaat tcgagattgt agacccggcg agggcgccct gggcggatga
     2281 gttcgcgaat gacttt
//
LOCUS       DAOPBQ010000561         2281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_37145_length_2281_cov_3.829290, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000561 DAOPBQ010000000
VERSION     DAOPBQ010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37145_length_2281_cov_3.829290"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKW60_14155"
     CDS             complement(<1..331)
                     /locus_tag="PKW60_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00624.1"
                     /translation="MKKDSKTMVSRREFAKASAAAISTFAVLRGASAQGSSEVLKVGL
                     LGCGSRGTGALTQMLQGNDNVKVIALADVFPDKVERTRTQIYSTDEFAQKRDIKDDHC
                     FTGLDAYK"
     gene            complement(528..884)
                     /locus_tag="PKW60_14160"
     CDS             complement(528..884)
                     /locus_tag="PKW60_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00625.1"
                     /translation="MGFWMAKMFADGMSAMDRAPGAKSDLIPQLITQTRELEHQVARM
                     ALLNQSLWELLCERLGLTEQELEAKVREVDLRDGVLDGKITNTPVKCPQCGRVSASKH
                     YRCLYCGLEFEKPVMG"
     gene            complement(907..1983)
                     /locus_tag="PKW60_14165"
     CDS             complement(907..1983)
                     /locus_tag="PKW60_14165"
                     /inference="COORDINATES: protein motif:HMM:NF022594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2920 family protein"
                     /protein_id="HPK00626.1"
                     /translation="MRALVLSVGCLMLAGVPAYGDAMAAWPALPDTDGDCLIPAQEWP
                     WQPGPRDVRVYIRYPGGTQAQVTAETGLFLSLHNWDGTFHTGTADPEALANRYNVVAV
                     CVDYLQSGPSEEEHPPYDFGYLQALDALRGLYFVYHGLEERGVAFHKGRLYCTGGSGG
                     GNVTLMANKLAPRTFACCIDKCGMARLTDDIAFNLKGGSRLNARYRSDPESPRYLSHG
                     AQMLRFVGHPEHLATMKQLGNTARMIVVHGADDDVCPIEDAREMVANMESAGLDVEAV
                     WVTSEMLDGDTFTSTDHSLGDRTRIVFRVGDACLMPGSPSLAVREGPADFERKESIRY
                     EVPGGAYVISYENGYPAGSFERDN"
     gene            complement(2001..>2281)
                     /locus_tag="PKW60_14170"
     CDS             complement(2001..>2281)
                     /locus_tag="PKW60_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPK00627.1"
                     /translation="WQDLGARIGGPCMIQLPDGRFAGAVRLYDERARTAICLIEPEYG
                     TLTEVLTLPSGGDTSYPGLVWHDGLLWVSYYSSHEGKTSIYLAKVAFN"
BASE COUNT          419 a          756 c          679 g          427 t
ORIGIN      
        1 ccttgtaggc atcgagcccg gtgaaacaat ggtcgtcttt gatgtcgcgt ttctgggcga
       61 actcgtcggt gctgtagatc tgcgtgcggg tgcgttcgac tttgtcgggg aatacgtcgg
      121 ccagagcgat aaccttgacg ttgtcgttgc cctgcagcat ctgggtcagg gcgccggtgc
      181 cgcggctgcc gcaacccagc agtcccactt tcaacacttc cgacgagccc tgagccgagg
      241 cgccccgcaa gacggcgaac gtcgagatgg cagcggccga agccttcgca aactccctgc
      301 gtgaaaccat ggttttcgaa tccttcttca tgagtcgttt cctccgctct gagctgatgt
      361 cggcaagcca gcgttcaaag cataaatttg gttgaagccc caggcccccg agccgggcgc
      421 caggaagttg agcataccaa tcgccaggtt cgcggcgcaa acaaccccgc cagccggcgc
      481 cgtggtccgg cgcgattccc ggcgaaggca acggggcaca aacggcctca gcccatcacc
      541 ggtttttcaa actccaggcc gcaatacagg cagcggtagt gctttgaggc ggagacccgc
      601 ccgcactgcg gacacttgac gggcgtattg gtaattttcc cgtcgagaac cccgtcgcgc
      661 agatcaacct cgcgaacctt ggcttcgagc tcctgttcgg tgaggccgag gcgctcacac
      721 agcagctccc acagagactg gttcaggagc gccatgcgcg cgacctgatg ctccagctcg
      781 cgtgtttgtg tgatgagctg ggggatcaga tcgcttttcg cgcccggggc ccggtccatc
      841 gcgctcatac catccgcgaa cattttagcc atccagaaac ccatacggca tcctccacgc
      901 ccggccttaa ttgtcccgct cgaaacttcc tgcaggatac ccgttttcat aggagatcac
      961 atacgctccg cccggtacct cgtagcggat ggactctttg cgctcgaaat cggcggggcc
     1021 ttcgcgcacc gcgagactgg ggctgcctgg catcagacag gcgtctccca cgcgaaaaac
     1081 aatgcgggta cggtcgccca gggaatggtc cgtggaggtg aaggtgtcgc cgtcgagcat
     1141 ctccgaggtc acccagaccg cttcgacgtc aagtcccgcc gattccatgt tggccaccat
     1201 ctcccgggcg tcctctatgg ggcacacgtc gtcatccgca ccatgcacca cgatcatcct
     1261 ggcggtattt ccgagttgct tcatggtggc caggtgttcg ggatgaccca caaagcgcag
     1321 catctgggcg ccatggctca gataacgcgg gctctccggg tcggatcggt accgtgcgtt
     1381 gagccggctg ccgcccttga gattgaacgc gatatcgtcg gtcaaacgcg ccatcccgca
     1441 cttgtcgatg cagcatgcga acgtgcgcgg cgccagtttg ttggccatga gggtcacgtt
     1501 gccgccaccc gaaccgccgg tgcaatagag gcgccctttg tggaacgcca cgccgcgctc
     1561 ttccagcccg tgatacacaa aatacaggcc ccgcagcgcg tccagcgcct gcaggtatcc
     1621 aaaatcgtag ggcggatgct cctcctctga cgggccgctc tgcaggtagt ccacacacac
     1681 cgcgaccacg ttgtagcgat tggccaaggc ctccgggtct gccgtgcccg tatgaaacgt
     1741 gccgtcccag ttgtgcaggc ttagaaacag gccggtttcg gcggtcacct gggcctgcgt
     1801 tccgcccgga taccggatgt agactcgtac gtcgcgcggc cccggctgcc agggccactc
     1861 ctgcgccggg atcaggcagt cgccgtcggt gtccggcagc gcgggccagg cggccattgc
     1921 atcaccatac gctggaacgc cagccagcat gaggcatccc acgctcagga caagtgcacg
     1981 catcgtccag gtttccttcc tcagttgaac gccactttgg cgagatagat gctcgtttta
     2041 ccctcgtgcg acgaatagta gctcacccag agcaggccat cgtgccagac cagccccgga
     2101 taactcgtgt cgcccccgga cggcagggtg aggacttcgg tcagcgtgcc gtactccggc
     2161 tcgatcagac agatggccgt ccgcgcgcgc tcgtcataca gccgcacggc cccggcaaag
     2221 cggccgtccg gaagctgaat catgcacggc ccgccaatgc gcgcgccgag atcctgccag
     2281 g
//
LOCUS       DAOPBQ010000562         2281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_37146_length_2281_cov_3.751123, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000562 DAOPBQ010000000
VERSION     DAOPBQ010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37146_length_2281_cov_3.751123"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PKW60_14175"
     CDS             <1..876
                     /locus_tag="PKW60_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HPK00628.1"
                     /translation="ALRVAADFIHTQLPGARVWLSDPTWVNHNSIFEAAGIKAASYPY
                     YDAEAHGLAFDALANALRSVPAGDVVLLHGCCHNPTGVDPTPEQWQLLSGICGQAGWL
                     PFFDFAYQGLGNGLEEDAAAVRAFTTPGRDMLVASSFSKNFGLYCQRVGALSVVASTP
                     QAARAAQSHVKRAIRANYSNPPAHGARLVIAVLGDPELHALWTRDVEAIRDRINTMRR
                     LFVDTLKEKGITQDFSFIQNQKGMFSFSGLTKDQVDALRTHFGIYIVGSGRINVAGMT
                     EANMDYLCEAIAHVL"
     gene            1020..>2281
                     /locus_tag="PKW60_14180"
     CDS             1020..>2281
                     /locus_tag="PKW60_14180"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPK00629.1"
                     /translation="MTLAIVMAASAFAAVDVPAPEGALPVRISSPWTLVLGPGRLDTP
                     DGARELKEAVSVAIAPMELQQIRGEEHKPLPVFDPKAGSWRRGSKLKQLIAEECTGTG
                     LAVPASIRLYAQPDGTGEFVEGTDYVVDAFWAQIGRIEGGGIDPEQAVYVDYDYYPTR
                     VDSILMDASGNLRVVQGQEGVGNIHLAEPGPGEIAVCTVWYNGILEGLEPENVFPIQF
                     SAPGASLSTPGAERLLPKTLAKLRNGEPVTIVAWGDSVTNGGGLKPGQEALRYQQQFL
                     TRLQERFPKSAITMHTAAWPGYDSKSYMNAPAGGPYDFQRDVLDQKPDLVIIEFVNDA
                     SLHGEALNEQYARIRDALWGISAEIILITPHYVRQDWMDVTTVKVDDDPRPYVQDLRK
                     FASQNDIAVADAARLWGALWRRGIPYPI"
BASE COUNT          440 a          739 c          674 g          428 t
ORIGIN      
        1 gcgctgcggg ttgcggcaga cttcattcac acccagcttc ccggggcgcg cgtgtggctg
       61 agcgacccaa cctgggtcaa ccacaacagc atttttgagg cagccggcat caaagccgcc
      121 tcgtacccct attacgacgc cgaagcccac ggcctggctt ttgacgccct ggccaatgcg
      181 ctcaggagcg tgccggcggg cgatgtggtg ctcctgcacg gctgttgcca caaccccacc
      241 ggtgtggacc ccacgcctga gcagtggcag ctactttccg gcatctgcgg ccaggccggg
      301 tggcttccct ttttcgactt tgcctaccag ggcctgggga acgggctcga agaagatgcc
      361 gccgccgtcc gcgccttcac cacgcccggg cgcgacatgc tggtcgcatc gagtttctcg
      421 aaaaacttcg gcctgtactg ccagcgcgtc ggcgccttgt cggtggtcgc ctcgacgccg
      481 caggccgcga gagcagcgca gagccacgtc aagcgtgcca ttcgggccaa ctactccaac
      541 ccgccagccc acggcgcgcg cctcgtgatc gccgtgctgg gcgaccccga gcttcatgcc
      601 ctgtggacac gggatgtcga ggccatccgc gatcgcatca acaccatgcg gcggctgttc
      661 gtcgacacgc tcaaagaaaa aggcatcacg caggatttct cgttcatcca gaatcagaaa
      721 ggcatgttct cgttttccgg gctcaccaag gatcaggtag atgcactccg gacacacttc
      781 gggatctata tcgtgggttc gggccgaatc aatgtggccg gaatgacgga agccaacatg
      841 gattacctct gcgaggccat tgcacacgtc ctttaagcaa accagccggg agcgttatct
      901 ggccgaaaac gcatgtgtca tcggagcccc ggcaattcgt cttggcgatt tcgcctgcgc
      961 tatactggga cctgctcgta accgccactg aattcaacgg aatatccggg gagactgaaa
     1021 tgacgctcgc gattgtcatg gccgcttccg cctttgccgc tgtagatgtc cccgcgcccg
     1081 aaggcgcgct tccggttcgt atttcgagcc cgtggacgct tgttctgggc cctggccgtc
     1141 ttgatacgcc ggacggcgcg cgggaattga aggaggccgt ttccgtagcc atcgcgccca
     1201 tggaactgca gcagatacgc ggcgaagaac acaagcccct ccccgtgttt gatcccaagg
     1261 cgggctcctg gaggcgtggt tccaaattga agcagctcat cgctgaggaa tgcaccggca
     1321 cgggtctggc cgttccggca agcatccggc tttacgcaca acccgatggt accggcgaat
     1381 ttgtggaagg cactgactat gttgtcgatg cgttctgggc gcagattggc cgcatcgaag
     1441 gcggcggaat cgaccctgaa caggctgtat acgtcgatta cgattactat cccacccgcg
     1501 tcgacagcat cctcatggac gcgtccggca acctgcgcgt cgtccagggc caggagggcg
     1561 tcggcaacat ccaccttgcc gagcccggcc ccggcgagat cgcggtatgc acagtctggt
     1621 ataacggcat cctggagggc ctggagcccg aaaacgtctt ccccatccag ttctccgcac
     1681 ccggcgcctc gctttccacg cccggcgcgg agcgcctcct gccgaaaacg ctggcgaagc
     1741 tgcgcaacgg cgaaccggtc accatcgtcg cctggggaga cagcgtcacc aatggcggcg
     1801 gactcaagcc gggacaggaa gccctgcggt atcaacaaca gtttctcaca cggcttcagg
     1861 agcgattccc gaagtctgcg attaccatgc acaccgcggc ctggcccggt tacgacagca
     1921 agagctacat gaacgcgccc gcgggcggac cctacgattt tcagcgcgat gtgctcgacc
     1981 agaaaccgga tctcgtgatc atcgagttcg tcaacgatgc ctccctgcac ggcgaggccc
     2041 tgaatgagca atacgctaga attcgcgacg ctttatgggg catttcggcc gagatcattc
     2101 tgatcacgcc gcattacgta cgccaggact ggatggatgt gaccacggtc aaggtggacg
     2161 atgacccccg tccctacgtg caagatctgc gcaagttcgc gagccagaac gatattgccg
     2221 tcgccgacgc cgctcgtctc tggggtgcgt tatggcgcag aggcatcccg tatcccatca
     2281 t
//
LOCUS       DAOPBQ010000563         2254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_37762_length_2254_cov_3.875398, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000563 DAOPBQ010000000
VERSION     DAOPBQ010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37762_length_2254_cov_3.875398"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKW60_14185"
     CDS             <1..443
                     /locus_tag="PKW60_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00630.1"
                     /translation="SLPTLSPVPSAERLMKASDPVNDPPHSLWDHFKRAFRRQNARRP
                     VSFYLMLAILAVLLLGLQLADYREQPRRFALVLSVMFIFFFIVLWRAAVEAMDIYRRS
                     CREERELFRSTLGNPEFNEKLGKRIRESASAVPPDDDAPATSDE"
     gene            436..1464
                     /gene="waaF"
                     /locus_tag="PKW60_14190"
     CDS             436..1464
                     /gene="waaF"
                     /locus_tag="PKW60_14190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HPK00631.1"
                     /translation="MSDAPARILAFVPNWLGDTAMATPALRALARRFPDSPLTAVGRR
                     SACALLHGLPWLHDTLSIPAQPGPLAMLALRKRISGATNTLAVVFPHSFRAALMARLT
                     GARTRLGYDRNGRAWLFTHRVQPHREQGAIVPIYMTDEYLGLVKTIGCEDDGAGLELA
                     ADAETIAQLRPRLAGDGPLVAVAPGAAFGPSKRWPAERYAAVMDALHEEKGCRIVLLT
                     GPGEEDTRDAVLAAVRHAPIIADEGKPTIDTLKATIALVDLLIGNDSGPRHVAIAFKK
                     PVICIMGSTSPLYSTGPYEIGEVLRVDVDCGPCQKPVCATDFRCMTRIAPAAVLAAAL
                     RHLPAVRS"
     gene            complement(1478..>2254)
                     /gene="rfbA"
                     /locus_tag="PKW60_14195"
     CDS             complement(1478..>2254)
                     /gene="rfbA"
                     /locus_tag="PKW60_14195"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HPK00632.1"
                     /translation="MIYYPLSVLMLAGIREILIISTPEDTPSFQHLLGDGSQLGLSFS
                     YVVQPRPEGLAQAFILGADFIGDDPVAMILGDNIFYGHNLVESLRRGSQLREGGLIFG
                     YPVRDPERYGVVEFDDQGNVLSIEEKPARPKSQYAVPGLYFYDSTVAEIARELKPSPR
                     GELEITDVNLAYLERGQLQVELLGRGFAWLDTGTHESLVQATNFVQTVQERQGLRIAC
                     IEEIAYRSGFIGEEQLRALAEPMRKNEYGAYLLRLLDGAV"
BASE COUNT          395 a          803 c          652 g          404 t
ORIGIN      
        1 tgtccctacc aacgctgagc ccggtgccca gtgcggagcg gctgatgaaa gcatccgacc
       61 ccgtaaacga cccgccccat tcgttgtggg atcatttcaa gcgagctttc cgccggcaaa
      121 acgcccgccg gcccgtcagt ttctatctca tgctggcgat cctcgccgtt cttctgctgg
      181 gcctgcagct ggccgattac cgggagcagc cacggcggtt tgcgctcgtt ttgtccgtga
      241 tgttcatctt tttcttcatc gtgctgtggc gcgcggcggt cgaggcgatg gatatctacc
      301 gccggagctg ccgcgaggaa cgcgagctct tccgcagcac cctggggaac ccggaattca
      361 acgaaaaact cggcaaacgc atccgggaaa gcgcctctgc ggttccgccc gacgacgatg
      421 cgcccgcgac ttcagatgag tgacgccccg gcccgaatac tcgcctttgt ccccaattgg
      481 ctcggcgaca cggccatggc cacgcccgcc ctgcgcgcgc tcgcacggcg ctttcctgac
      541 agccccctga cggccgtcgg cagacgcagc gcctgtgcgc tgctgcatgg gctcccctgg
      601 ctgcatgata ccctgtccat tcccgcccag cccggcccgc tcgccatgct cgccctgcga
      661 aagagaattt ccggcgccac caacaccctg gcggtagtgt tcccgcactc cttccgcgcc
      721 gcactgatgg cccggctcac cggcgcgcgc acccgcctgg gctatgaccg caacggccgc
      781 gcctggctct tcacccaccg ggtccagcct catcgtgaac agggtgcaat cgtgccgatc
      841 tacatgacag acgaatacct cggcctcgtc aagaccatcg gctgtgaaga cgatggcgcg
      901 ggcctcgaac ttgccgccga tgcggagacc atcgcccagc ttcgtccacg tctcgccggc
      961 gacggcccgc tggtcgccgt ggcccccggc gccgcgttcg ggcccagcaa acgctggccc
     1021 gccgagcgct atgccgccgt catggacgcc ctgcacgaag agaagggctg ccgcatcgtc
     1081 ctgctgaccg ggccggggga agaagacacc cgcgatgccg tcctggccgc cgtccggcac
     1141 gctcccatca ttgcggacga gggcaaaccc accatcgaca ccctgaaagc cacgatcgcc
     1201 ctggttgatt tgctgatcgg caacgacagc ggtccgagac acgtggcgat cgcattcaaa
     1261 aagcccgtga tctgcatcat gggctccacg tcgccgctct attccacggg tccgtatgaa
     1321 atcggtgagg tgcttcgcgt ggacgtggat tgcgggccct gccagaagcc cgtgtgcgca
     1381 accgatttcc gttgcatgac ccgcatcgcc cccgccgccg tcctggcggc cgccctgcgc
     1441 catttgcccg ctgttcgaag ctgaaagcag gccgccgcta cacggcgccg tccaagagcc
     1501 gcagcagata ggccccgtat tcgtttttcc gcatgggctc ggccagcgcc cgcaactgtt
     1561 cctccccgat gaacccggag cggtatgcga tctcctcgat gcacgcaatg cgcaagccct
     1621 gccgctcctg gaccgtctgc acgaagttcg tcgcctgcac cagcgattcg tgcgttcccg
     1681 tgtccagcca ggcaaatccg cggccgagca gttccacctg caactggccg cgttcgaggt
     1741 aggccaggtt tacatcggtg atttccagct cgccccgtgg cgaaggtttc agctcccggg
     1801 cgatttcggc caccgtactg tcatagaagt agagcccagg caccgcgtac tgagacttgg
     1861 gccgcgccgg tttttcttcg atgctcagca cattcccctg atcgtcgaac tccaccacgc
     1921 cgtagcgctc cgggtcgcgg accggatacc cgaagatcag cccgccttcg cgaagctgcg
     1981 acccgcgccg gagcgattca accagattgt gcccataaaa aatgttgtcg cccaggatca
     2041 tcgctacggg gtcgtctccg atgaagtccg ccccgaggat aaacgcctgg gcaagccctt
     2101 cgggccgtgg ctgcaccaca taagaaaacg acagtcccag ttgcgaaccg tcccccagaa
     2161 ggtgctggaa cgacggcgta tcttccggcg ttgaaatgat gaggatctcg cgaatgcccg
     2221 ccagcatcag caccgagagc gggtaataaa tcat
//
LOCUS       DAOPBQ010000564         2251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_37843_length_2251_cov_2.353370, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000564 DAOPBQ010000000
VERSION     DAOPBQ010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37843_length_2251_cov_2.353370"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="PKW60_14200"
     CDS             <1..1179
                     /locus_tag="PKW60_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPK00633.1"
                     /translation="RSAKQPEIGVLIDDAMEAIEKENPGLKGVLPKDYARPTLDKQRL
                     GELIDLIGTIALGGIENRSKDILGRVYEYFLNEFARAEGRRGGEFYTPRCVVAVLVEM
                     LAPYKGTVFDPCCGSGGMFVQSESFVEAHGGRRVDISIYGQESNPTTWRLAKMNLAIR
                     GIEANLGAEPADSFHRDLHKSLKADYILANPPFNMSDWGGSRLCDDVRWKYGIPPVGN
                     ANFAWVQHFIHHLAPRGIAGFVLANGSMSSYTSGEGEIRKAIIEADLVDCMLALPGQL
                     FYSTQIPVCLRFIARDKKNSRFRDRRGQTLFINARRHPLELLDFGELLDDALKAMSKL
                     LDDPKASALTKLRFIEEIFDRDPPAPSPNAPSLSRRPTGPAPSLTTRPSGSSKRGPLS
                     "
     gene            1923..>2251
                     /locus_tag="PKW60_14205"
     CDS             1923..>2251
                     /locus_tag="PKW60_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00634.1"
                     /translation="MSRTYKVPWGVHCISAACLDDDARGQPLEAIRQLELDTKILDES
                     VWESIGQRGFGLPRYFSAYVHALRWNCVTATDARLFQAPFCAGIRIDSYQLEPLRKAL
                     LLPRVNL"
BASE COUNT          487 a          739 c          602 g          423 t
ORIGIN      
        1 cgcagtgcca aacagcccga gatcggcgtc cttatcgacg atgccatgga ggccatcgag
       61 aaggaaaacc ccggcctcaa aggcgtcctg cccaaggatt acgcccgccc gaccctcgac
      121 aagcagcgcc tgggcgaact catcgacctc atcggcacca tcgccctcgg cggcatagaa
      181 aatcgctcca aagacatcct cggccgcgtc tacgagtatt tcctcaatga atttgccaga
      241 gccgaaggca gaagaggcgg cgagttctac accccccgct gcgtggtcgc ggttctcgtc
      301 gagatgctcg cgccctacaa aggcacggtg ttcgacccct gctgcggctc cggcggcatg
      361 ttcgtccagt ccgagtcctt cgttgaggcc cacggcggcc gacgcgtcga tatcagcatc
      421 tacggccagg aatctaaccc caccacctgg cgcctggcca aaatgaacct cgccatccgc
      481 ggcatcgaag ccaacctcgg cgccgaaccc gccgacagct tccaccgcga ccttcataaa
      541 tccctcaagg ctgactatat cctcgccaat cccccgttca acatgagcga ttggggtggg
      601 agccgcctgt gcgacgatgt ccgctggaaa tacggcatcc cgcccgtggg aaacgccaac
      661 ttcgcctggg tccagcattt catccatcac ctcgcgcccc gcggcatcgc gggcttcgtc
      721 ctcgccaacg gctccatgtc ctcctacacc tcaggcgaag gcgagatccg gaaagccatc
      781 atcgaggccg acctggtgga ctgcatgctc gcgctccccg gccagctctt ctactccacc
      841 cagatccccg tctgcctgag gttcattgcc cgcgacaaaa agaacagccg tttccgcgac
      901 cgtcgcggac agacgctctt catcaacgcc cgcaggcacc ctctggaatt gctggatttc
      961 ggcgaactgc tcgacgatgc actgaaagcc atgagcaagc ttctggacga tcccaaggcg
     1021 tccgcactga ccaaactccg cttcatcgaa gaaatcttcg accgcgaccc accggcgcct
     1081 tcgccaaacg cacccagcct gtcacgccgg ccaaccggcc cagccccgtc tttgacaacg
     1141 aggccatccg gaagctcaaa gagagggccg ctgagctgag agaatcctcc cgttcttagc
     1201 acgatagccc gacaatcacg gagtaccacc ctttctccct ggttgttacg agcaagcctc
     1261 ccgaatcttc ggatagctcc agagctgggt tttactctgg accaggtgga ttcatcagtg
     1321 ctgcgccttg gctgtgcagc ctcgccgacg ccttccgcag aataccggga acgtcgaatt
     1381 gcctactctc ggagctctcg gtcgggcata ccgtcaatct ctgacgaatc gtcaaaccga
     1441 ggggcaaaac ccttatattt atatataaac agcaagacaa aaggcacccg tcttgcaggg
     1501 agagggggct tgaaaacgcc cccctctccc actgacagac ggtagtggtt ttgacccact
     1561 gaccagctgg aaatcaaaaa aagcccgtaa gactcgaaat tcaaaaaata tagaaatttg
     1621 gcgcttgtga cagtagcagc tcacccatgg cgcgcgagtc tcccaggcgg gcgggcgggc
     1681 gcggcggcgg atacgtaggc ggccccacgc ccacgagggt agtgaagcca gactggccag
     1741 aaacggcgcg cggcaaggtg aagaactggg agagatatca tgtttatatt ccatttagtc
     1801 ttatgagtaa gattgcgcat ggggtaattc tgttacatca aaaaggtcac gggagatgcc
     1861 gactttctct gcggggactc cgtcgccggg cgacatcgta cgcattcgca cgaggacaca
     1921 ccttgtcgag gacgtacaag gtgccgtggg gggtacattg catctctgcg gcgtgcctgg
     1981 acgatgacgc ccggggccag ccgctcgaag cgatccggca acttgagctc gacaccaaaa
     2041 ttctcgacga atccgtctgg gagtccatcg gtcagcgcgg tttcggcctg cctcgctact
     2101 tcagcgccta cgttcatgca ttgcgatgga actgtgtcac cgccactgat gcgcgtctgt
     2161 tccaggcgcc cttctgcgcg ggtatccgaa ttgactccta tcaattggag ccgctacgga
     2221 aagccctgct gctcccgcgc gtcaatcttt t
//
LOCUS       DAOPBQ010000565         2250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_37856_length_2250_cov_4.171754, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000565 DAOPBQ010000000
VERSION     DAOPBQ010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37856_length_2250_cov_4.171754"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PKW60_14210"
     CDS             <1..649
                     /locus_tag="PKW60_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00635.1"
                     /translation="INITILVAAQSELVVPVSCVEQGRWSSVSRRFRATHFATPHVRA
                     RKCVRVMESVACHGSFGGDQSEVWHDVAQELRFARAASRTASLTDGIEATRSTAEEYR
                     RNLALPEDAAGVAIATDSRVVLFELFDTPRTMRRLWPRLSEACFFTFAHDESTRKPAP
                     ERVIRDFADGVRGNLQVLSETRGLGVGLGFSGRKMSGTGLCYEDRLLHLAAFPLD"
     gene            complement(806..>2250)
                     /locus_tag="PKW60_14215"
     CDS             complement(806..>2250)
                     /locus_tag="PKW60_14215"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPK00636.1"
                     /translation="PDDRGDLLAWAEGGPLLRNGVPTAPAWPASLDSDCVVLLSQSAD
                     KSPGANAACIAPMRYRAMPSIAYRLALAAAGDGVAGASLYGPGTWDFAGGHALLLGAG
                     GDLFSRDGTPVRYDPDGSCRSSAWVFGGSPPHVDHIRGSDWSAVLDEVPADGPYGLCF
                     PRSGGGPLDPAMHARAQGCLLGQLAGDALGSLVEFQGPDAIAARYPGGVRELADGGTH
                     HTIAGQPTDDSEMALLLARSLIEHGAYEPDPVRHAYVYWLESGPFDCGITIGNALRGG
                     YNYESQANGALMRISPLGIFGVHHAPHELADWARQDAVLTHPHPVCQEVNALFAMAIA
                     HAIRTGCGPGSLCDQIAEWAREMDCLPPVVETIEAARTAPPDDYITLQGWVLIAFQNA
                     LWQLQHASGFTEAVVDTVMRGGDTDTNAAICGALMGAMHGFDAIPAQWRDRVLTCRPI
                     GSLPDVHRPRPRCFWPVDALILVDRLLAAG"
BASE COUNT          393 a          736 c          759 g          362 t
ORIGIN      
        1 tatcaacatc accatcctcg ttgcagcgca atccgagctc gtggttcctg tgagctgcgt
       61 cgaacagggc cgctggtcca gcgtcagccg gcgatttcgc gccacccact tcgctacgcc
      121 ccatgtccgc gcccggaaat gcgtgcgggt gatggagtct gttgcgtgcc acggctcctt
      181 cggcggcgat cagtcggaag tgtggcacga cgttgcgcag gaactgagat ttgcccgggc
      241 cgcatcgcgc acggcatcgc tgaccgacgg gatcgaggcc acgcgcagca cggccgagga
      301 ataccggcgc aacctggcgc ttcctgaaga tgctgccggc gtcgccatcg ccaccgattc
      361 gcgtgtggtg ctcttcgaac tcttcgatac cccccgcacc atgcggcgtc tgtggccccg
      421 gctcagtgag gcctgtttct tcacctttgc ccacgacgaa agcacccgca agccggcccc
      481 cgagcgtgtg atccgcgatt ttgccgacgg ggtgcggggc aacctccagg ttctctccga
      541 aacccgcgga ctgggcgtag gcctcggatt cagcggccgg aaaatgtccg gcaccggcct
      601 ctgttacgaa gaccgtctgc tgcatctcgc ggcttttccg ctcgattaac gggtttcatg
      661 ccccggccca acgttcttcc ggcccgtcag cgtaactgcg ctggcgggcc gctttctgag
      721 caggacacgc gagcacacgg cacgggaact cttaaacggg taagcaacaa gagcgccacc
      781 ggcggatcgc gggtgatgcc gcgccttatc ccgccgcaag aaggcgatcc acgagtatca
      841 gggcgtcgac aggccagaaa cagcgtgggc gggggcggtg cacatccggc aggctgccaa
      901 tgggccggca ggtgagaacg cggtcgcgcc attgcgcggg aatggcgtcg aatccgtgca
      961 tcgcgcccat cagcgccccg cagatggctg cattggtatc cgtatcgcca ccgcgcataa
     1021 cggtatcgac gaccgcctcc gtaaacccgg acgcgtgctg gagctgccag agggcgttct
     1081 ggaatgcgat gaggacccat ccctgcaggg tgatatagtc gtccggaggg gcggtccggg
     1141 cggcctcgat ggtctctacg accggaggca ggcaatccat ctcccgtgcc cactcggcga
     1201 tctggtcgca gaggctgccg ggcccgcatc cggtccggat ggcatgcgca atcgccatgg
     1261 caaacagcgc attcacctcc tggcacacgg gatgcggatg cgtcaggacc gcgtcctggc
     1321 gtgcccagtc ggcgagttca tgcggcgcat ggtgtacgcc gaaaatgccc aggggcgaaa
     1381 tacgcatcag cgcgccattg gcttggctct cgtagttgta accgcctctg agggcatttc
     1441 cgatggtgat accgcaatcg aacggaccgg attcaagcca gtagacgtag gcgtggcgca
     1501 cgggatcggg ctcatacgcc ccatgttcaa tgagcgagcg cgcgagcagc agcgccatct
     1561 ccgaatcgtc cgtgggctga cccgcaatgg tgtggtgggt gccgccgtcg gcgagttcgc
     1621 gcacaccgcc gggataacgc gcggcgatgg catcgggtcc ctgaaattcc acgagactgc
     1681 caagcgcgtc gcccgccagc tggccgagca ggcatccctg ggcgcgggcg tgcatggcgg
     1741 gatcgagggg gccgccacct gagcgcggga agcaaagccc gtacggccca tccgcgggga
     1801 cttcatccag cacggcggac caatcgctcc cccggatgtg gtcgacatgg ggcggtgagc
     1861 cgccgaacac ccaggccgat gaccggcagg aaccgtcagg gtcatagcga acgggagtgc
     1921 catcccggct gaacaggtcg ccgcctgcgc caagcaacag ggcatgtcct ccagcgaaat
     1981 cccaggtgcc cgggccgtag agggatgcgc ccgcgacacc gtcgcccgcg gcggccagtg
     2041 caagccggta cgcgatgctg ggcatggcgc ggtagcgcat gggggcgatg cacgctgcgt
     2101 tggcgccggg gcttttgtct gcgctttgcg agagcagaac gacacaatcg ctgtcgaggg
     2161 atgcaggcca ggccggtgcg gtggggacgc cattgcgcag cagcgggccg ccctcggccc
     2221 aggcaagcag atcgccccgg tcgtcgggat
//
LOCUS       DAOPBQ010000566         2250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_37863_length_2250_cov_3.297950, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000566 DAOPBQ010000000
VERSION     DAOPBQ010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37863_length_2250_cov_3.297950"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2001
                     /locus_tag="PKW60_14220"
     CDS             <1..2001
                     /locus_tag="PKW60_14220"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPK00637.1"
                     /translation="LGCDKDFSLESPLKSTDTPASPSPWVYASFEEAKFSATDLEWSE
                     WPRDQQNAVREKLDGIARDHIEHSGTLDAFLARLGTAATMLEASQVTEDVHGDFMRRD
                     VISAANVDAPEAAITGTGNTMVHPDASGDVELIYDLGEQNIGYWQLDVNANAGAIIDL
                     AGVEYINPEGKVQHTDRYRNDMRYICKEGDNQFTSLMRRSGRYLFLTFRNLQRPAILR
                     AVSVVESTYPVEPVGSFACSDARLNTIWEISARTLKLCMEDTFTDCPLYEQTHWVGDA
                     RNEALLGFTAFGAPDLARRCVKLTAYSLDHQPYVLGEKAGVPLEHLLALQHPLTISQT
                     PSTWDIVIPVWSFLWTISAWDYYYFSGDAEFLQWVYPYVLKNMRSAESLTDERGLFSA
                     PYWNMFDWAGIDDAARTVTHNSMFAVGAADAALKCAQVLGDQDGAKWLREYRQRLSQS
                     INALWNSQTEWYPDSIRDDGNPSPKTCVHTGFLSLLYDIAPEDKHAEILDKVLNPPEG
                     MTQVGSPFAVLYLFEMLEKAGRADEIIARIHQYYQPMLDLNATTVWETFANSPGRLQD
                     FPTRSHCHAWSSSPIHFLNRIVLGIVPVSAGGTRFSISPRPSGLDWARGASASIHGPV
                     EVSWHKEGETIEIEAKAPKSVELEFVDNESLQGFKVNYRRTE"
BASE COUNT          452 a          692 c          654 g          452 t
ORIGIN      
        1 ctcgggtgtg acaaagactt ttcgctcgaa tccccgctca aatccacgga tacgcctgcc
       61 tcgccgtccc cgtgggtcta tgcctcgttt gaggaggcaa aattctcagc gaccgacctg
      121 gaatggtcgg aatggccccg ggaccagcaa aacgccgtga gagaaaagct cgacggcatc
      181 gcccgggatc atattgagca cagcggcacg ctggacgcct tccttgcccg cctgggtact
      241 gcagcaacga tgctggaggc ctcgcaggta accgaagacg tgcacggcga cttcatgcgg
      301 cgagacgtga tctcggccgc caacgtggat gcgccggaag ccgcgataac cggcacaggg
      361 aataccatgg ttcatcctga cgcctcgggc gacgtcgagc tgatttatga cctgggcgag
      421 cagaatatcg gctactggca gctggatgtt aatgcgaatg cgggggccat catcgacctc
      481 gccggcgtcg agtacatcaa tccggaaggc aaggtgcagc acacggaccg ctaccgcaat
      541 gacatgcgct atatctgcaa agagggcgac aaccaattca ccagcctcat gcgccgttct
      601 ggccggtatt tgtttctgac tttccgtaac cttcagcggc cggccattct tcgggccgtc
      661 agcgtggtcg aatccaccta tcccgtcgag cccgtgggct ccttcgcctg ctccgacgcg
      721 cggctcaaca cgatttggga gatctcagcc cgcacgctga aactctgcat ggaagacacg
      781 ttcacggact gtccgttgta cgagcaaacg cattgggttg gcgacgcccg caatgaagcc
      841 ctgctcggtt tcactgcctt tggcgcaccc gacctggcgc gccggtgcgt caagctcacc
      901 gcgtactcgc tcgaccatca gccgtatgtg ctgggagaaa aggccggagt ccccctggaa
      961 cacctgctgg cgctgcaaca tccgttgacc atctcccaga cgccaagcac ctgggacatc
     1021 gtaattcccg tgtggagttt tctctggacc atctcggcct gggattacta ctacttttcc
     1081 ggcgacgccg agtttctcca gtgggtctat ccctacgtcc tcaagaacat gcggagcgcg
     1141 gaatcgctga cggacgagcg ggggctgttc agcgccccgt attggaacat gttcgattgg
     1201 gccgggattg atgacgccgc ccgaacggtc acccacaaca gcatgttcgc ggtgggcgcc
     1261 gcggatgccg cgttgaagtg cgcccaggtt ctgggcgatc aggacggtgc gaaatggctg
     1321 cgcgaatacc gtcaacgcct ttcccagtcc atcaatgcgc tctggaacag ccagaccgaa
     1381 tggtatcccg acagcatccg cgatgatggc aatcccagcc ccaaaacgtg cgttcacacg
     1441 gggtttctgt cgttgctcta cgatatcgcg cccgaggaca aacacgcgga gattctcgac
     1501 aaggtgctca acccgcctga gggcatgaca caggtcggct cgccgtttgc cgtgttgtat
     1561 ttgtttgaaa tgctcgagaa agccgggcgt gccgacgaaa tcatcgcgcg gattcaccaa
     1621 tactaccagc ccatgctgga tctcaatgcc accaccgtct gggagacctt cgccaacagt
     1681 ccgggcaggc tgcaggattt cccgacgcgg agccactgcc acgcctggtc ctcatcgccg
     1741 atccatttcc tcaaccggat agtgctcggc atcgttcctg tctccgccgg cggcacgcgt
     1801 ttcagcatca gtccgcgccc ctccggactg gattgggccc ggggcgcgag cgcgtcgatc
     1861 cacggcccgg tcgaggtgtc ctggcacaaa gaaggtgaaa ccattgagat cgaggccaaa
     1921 gcgcccaaat ccgtcgaact ggagtttgtt gataacgaat ccctgcaagg attcaaggta
     1981 aattaccgcc gaacggaata atgatgcttc gtagaatgaa gatgcgagcc ccgttgggcc
     2041 ttcaaggcgc ccgtggtctt gtttcgcccg ccggggccgg cctggtatca caaagatgct
     2101 gattgccggc cggcagcatg gtagaatggc gttcttttgg ggaggattca ccataccatg
     2161 tggtcattaa attctctgga ctacactgtc attgtgggct atttcgcggc actggtggtg
     2221 ctcggcctgg ccctggaacg cctggcctca
//
LOCUS       DAOPBQ010000567         2247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_37918_length_2247_cov_4.548814, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000567 DAOPBQ010000000
VERSION     DAOPBQ010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37918_length_2247_cov_4.548814"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PKW60_14225"
     CDS             complement(<1..884)
                     /locus_tag="PKW60_14225"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00638.1"
                     /translation="MRAYYCPGTHWDREWYEPFQEFRMWLVELVDDLLDLLERQPEYK
                     CFHLDGQAVVLEDYLRVRPENRDRLVAQMRAGRILAGPWYVLPDEWLVSAESYIRNVM
                     KGRAVCRQLGVPAMRFAYTPDQFGHIAALPMVMRGLGLEAGICWRGAQDENYPALFMW
                     IAPDGSRMPTFKLRDDGSYAPFLFKFRNLLEKNQLTDELVLEHFGPYLQEERERANVP
                     LALLLDAIDHYPADEQSVAILRKLKDLYPDVEFIWTSLEEFGREMVACAEQLPERTGE
                     PREPCRTSGRGGQYLIVHT"
     gene            complement(958..>2247)
                     /locus_tag="PKW60_14230"
     CDS             complement(958..>2247)
                     /locus_tag="PKW60_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00639.1"
                     /translation="LPDQDMIPSYATPPQYNLARSNRRTTNLVGFGLMGALLLAAVMV
                     VIVRSGNRDEASPEPSMPLNQAEQMASETGEEMPAPELPLPQEEGFLEPPAAADSTPV
                     EQSLELPAAPPGDAPLSVEAQDETAAAEPLPPMPVESDPVESLASQIQHMDSIEQLKT
                     LSEMDAAQLAEVMEAQSKAAVNTLAEEASTLEERDKVAQMREKLENTNFQEAARAMKE
                     VLAQTDPYIFTGVSLSIFDEGLKAGASYTDELLRGFDFSKKSAAGQRESQDTAAEEEG
                     AETLPVEQPAEKPAPKAEKPAPEKPVRPIFGTTLARTIEFPGDESLGEISLRKTGALE
                     REPWERAAEARGKLGIPPGYDVKLQMHTRRLEHLEKLNADDIQVLSLWSLKIDDKKLG
                     PVKRLTGLKELDIRQTNVTMEGLEELRKALPSCRILY"
BASE COUNT          381 a          680 c          695 g          491 t
ORIGIN      
        1 gtgtggacga tgaggtactg gcctccgcgg ccactggtgc ggcagggttc gcgcggttca
       61 ccggtccgtt cgggcaattg ctccgcacac gccaccatct cccgcccaaa ttcttccagc
      121 gaggtccaga tgaactcgac atccggatac aggtccttca gcttgcgcag gatggctaca
      181 ctctgttcat ctgcggggta gtggtcaatg gcgtcgagca ggagcgccag aggaacgttg
      241 gcccgctcgc gttcttcttg aaggtatggc ccaaaatgtt ccaggaccag ctcgtcggtc
      301 aactggtttt tctccagcag gttacggaat ttgaagagaa atggggcata actgccgtcg
      361 tcgcgcagtt tgaaggtggg catccggctt ccgtcaggag cgatccacat gaacagcgcg
      421 ggatagttct catcctgcgc cccgcgccaa cagatgccgg cctccaggcc gagaccgcgc
      481 atcaccatgg gcagcgcggc aatatggccg aactggtccg gcgtatacgc aaaacgcatc
      541 gcaggcacgc cgagctggcg gcagaccgcc cggcctttca tgacgttgcg gatatagctt
      601 tccgcggaga cgagccattc gtccggaagc acataccagg gccccgcgag gatacgccca
      661 gcgcgcatct gcgccacaag ccggtcgcga ttctcaggcc gcacgcggag ataatcttcg
      721 aggacaaccg cctggccgtc caggtgaaaa cacttgtatt cgggctggcg ttccagcagg
      781 tcgagcagat cgtcgaccag ttcaacgagc cacatgcgga attcctgaaa cggctcatac
      841 cactcgcgat cccaatgagt tcccggacag taataggcgc gcattcgaat tacctttcgt
      901 ttccggccac gcggccggtt gtgagggggt ggcggctggg agatgctcct gccgcgctca
      961 atacagaata cggcacgacg gaagtgcttt gcgaagctct tccaggcctt ccatcgtgac
     1021 gtttgtctgg cgaatatcga gctctttaag tcccgtcagc cgtttgacag gcccgagctt
     1081 cttgtcgtcg atcttcaggc tccacaggct cagcacttgg atatcatccg cgttgagttt
     1141 ctcaagatgt tcgagccggc gcgtgtgcat ctgaagtttg acgtcgtagc cgggggggat
     1201 tccgagtttg ccccgggcct ctgccgcgcg ttcccagggt tcgcgttcca gggcgcctgt
     1261 tttgcgcagg gagatctcgc cgaggctttc atcgcccgga aactcgatcg tccgggccag
     1321 cgtagtgccg aaaatcggcc ggaccggctt ctcaggcgcg ggtttttccg cctttggggc
     1381 tggcttttct gccggttgtt ctaccggaag ggtctcggcg ccctcttcct cggcggccgt
     1441 gtcctggctc tcccgttgtc ccgcagcgct ctttttgctg aaatcaaagc cgcgcaaaag
     1501 ctcgtcggtg taactcgccc cggccttgag cccttcatcg aagatggaga ggctgacacc
     1561 cgtgaatatg tacggatccg tctgcgccag gacttccttc atcgcccggg cggcttcctg
     1621 gaaattggtg ttctccagtt tttcgcgcat ctgtgctact ttgtcccgtt cttcgagggt
     1681 gcttgcctcc tcagcgaggg tatttacggc ggctttggac tgggcttcca tcacctccgc
     1741 cagctgtgcg gcgtccattt cagacaaggt cttaagctgc tcgatcgaat ccatatgctg
     1801 tatctgcgac gccagagatt cgaccgggtc gctttcgact ggcatgggcg ggagcggttc
     1861 cgcggctgcg gtttcgtcct gggcttccac ggagagaggg gcatcgccag ggggggcggc
     1921 cgggagctcc agactctgct ccaccggggt cgagtcagct gcagccggag gctcgagaaa
     1981 gccctcttcc tgcggcaggg gcaactcggg tgctggcatc tcttcgccgg tctctgatgc
     2041 catctgctct gcctggttca ggggcatgga aggctcaggg ctggcttcgt ctcgatttcc
     2101 gctccgtacg atgacgacca tcaccgcggc gaggagcaag gcccccatca ggccgaatcc
     2161 cacgaggttg gtggttctgc ggttgctgcg cgccaggttg tactggggcg gcgtagcgta
     2221 ggaagggatc atgtcctgat ccggcag
//
LOCUS       DAOPBQ010000568         2239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_38088_length_2239_cov_4.412546, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000568 DAOPBQ010000000
VERSION     DAOPBQ010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38088_length_2239_cov_4.412546"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            541..2076
                     /locus_tag="PKW60_14235"
     CDS             541..2076
                     /locus_tag="PKW60_14235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HPK00640.1"
                     /translation="MWYKTKRVLLPAFFAAAVCGLVAVGALVFEPAPKTVYGADTSEA
                     IQTLKDFGNIFAEVAEVASPAVVSIQVSREVEASQMQEGYPEGLPDDLFEFFFGPRGF
                     GRRLPQPRGRAPRGFQMPRETPMGQGSGFVIDPSGYIVTNNHVVGDFKDGKIAVKLSD
                     GREFEAKLIGSDPKTDVALIKVEATGLPSLRLGDSDAIKVGEWVLAIGNPFGLTHTVT
                     AGIISARGRGNVGIVDYADFIQTDAAINPGNSGGPLINLDGEVVGMNTAIFSRSGGSM
                     GIGFAIPVNMIQYVETQLRESGTVTRGQLGVVIQPLTQELADSFGVDESKGILVGQVM
                     PGMAAEKAGIKQGDIILELNGKPVGDLNSFRSRIANTAPGTAITLVVLRDGKRLEKTA
                     TIGELDEEAVAAAGPAGTSGPAKVEKGIGITVQNLTDDVAAELGYENEQGVVVSSVDP
                     DGPAAMANIESGNLIQEVNRTKVTNIDEFQQAMERASKKETVLLLVSDGQFARYVALR
                     LAK"
BASE COUNT          492 a          661 c          692 g          394 t
ORIGIN      
        1 atggcgcgac gatatacgcc ccggtaagat tagcaaatgg cttgacagga ccggctgcaa
       61 gagtggtgca gcgggagcag cgtgccgaga ggcaagcggc cgccagaatc gctgaaaatg
      121 ggaatgacag gcgccggccg cgggtttgcg cataaggacg cagtcataaa atgtatatgc
      181 cggacaggcg ccctgggccg gcctgcccga atgcccgaga gtgcaaaaaa agtgaccaga
      241 aagggtgcgg gccttgccaa acgacggcga tcgagactgc gacagcgttt cagaagaaga
      301 cagacggcgg cctgaattga ggcatcacgt ctcgtatcag tcacaattca aatcgggccc
      361 catcccgggc agcgactcta aagctcttat attctgtgac ttagcgaaga cccgccggat
      421 tggcacgccg catgcgttat tccgggggtt tccgacaaag cgcaacaacg cactcgaacc
      481 ggatgcgttg ctgttccagc actcccggga ctgagtccga cgggaaagga gagcgagatt
      541 atgtggtaca agacgaaacg agttctgctg cccgcgttct tcgcagcagc ggtctgcggg
      601 ctggtagcgg tgggtgcgct ggtatttgaa cccgcgccca aaaccgtcta cggcgcagat
      661 accagcgaag ccattcagac acttaaagat tttgggaaca ttttcgcaga ggtggcggaa
      721 gtggcttcac cggcggtggt gtcgatccag gtgagccgcg aagtagaggc ttcccagatg
      781 caggaagggt acccggaagg gcttccggat gacctgttcg agttcttctt cggcccgcgg
      841 ggtttcgggc gccgcctgcc ccagccgcgc ggccgcgcac cgcgaggatt ccagatgcct
      901 cgcgaaaccc ccatgggtca gggaagcggt ttcgtcatcg acccaagcgg gtacatcgtg
      961 acgaacaacc atgtggtagg ggatttcaaa gacggcaaga tcgcggtgaa gctttccgat
     1021 ggacgtgaat ttgaggccaa attgatcggc agtgacccca aaacggacgt ggcgctgatc
     1081 aaagtcgagg caacgggcct gccctccctc cggcttggag attccgatgc catcaaggtg
     1141 ggcgaatggg tgctggccat cggcaacccc ttcggcctga cgcacaccgt cacggcaggc
     1201 attatcagcg cccgcggccg cggcaatgtc ggcatcgtcg attacgccga tttcattcag
     1261 accgacgccg ccattaaccc gggcaactcc gggggaccgc tcatcaacct cgacggggaa
     1321 gtggtcggta tgaacacggc catcttcagc cgcagcggcg gctccatggg tattggtttc
     1381 gccatccccg tcaatatgat tcagtacgtc gaaacccagc tgcgtgagag cggcaccgtg
     1441 acccggggac agctcggggt cgtcattcaa cctctgaccc aggaactggc cgatagtttc
     1501 ggcgtcgatg agagtaaagg catcctggtc ggccaggtca tgcccggcat ggcggctgag
     1561 aaagcaggga tcaagcaggg cgacatcatc ctggagctta acggtaagcc cgttggcgac
     1621 ctgaattcgt tccgcagccg aatcgccaat actgcgcccg gcacggcgat caccctggtg
     1681 gtgcttcgtg acggcaaacg cctcgagaag accgccacga ttggcgagct ggacgaagaa
     1741 gccgtcgccg cagccggccc ggccggcacc agcggacccg caaaagtgga gaaaggcatc
     1801 ggcatcaccg tccagaatct gaccgacgat gtcgctgccg aactcggcta cgaaaatgaa
     1861 cagggtgtgg ttgtatccag cgtggatccc gatggcccgg ccgcgatggc caacatcgaa
     1921 tccggaaacc tcatccagga ggtcaaccgc accaaagtca ccaatatcga tgagtttcag
     1981 caagcgatgg agcgcgccag caagaaggaa accgtgctgc tgctggtatc cgacggccag
     2041 tttgcgcggt atgtggcgct gcgccttgca aaatagataa cacctgcggt ctgttgcctc
     2101 agttcccgac cgtcagtacc tccccctgac gcagggcggc ccctcccccc aggggccgcc
     2161 cacccccatt ttccgggcga tcgcacctgt caagccattt gctaatctta ccggggcgta
     2221 tatcgtcgcg ccatttggt
//
LOCUS       DAOPBQ010000569         2235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_38185_length_2235_cov_3.232110, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000569 DAOPBQ010000000
VERSION     DAOPBQ010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38185_length_2235_cov_3.232110"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKW60_14240"
     CDS             complement(<1..313)
                     /locus_tag="PKW60_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HPK00641.1"
                     /translation="MKEKYSSRHKLSRRNFMATAGAAAGVMIASGYSPFSYAQNDRVR
                     VASIGTGGQGCLHLRDGLARAKNIQVVAVCDVYQPHLQGGWIVAGRDKNMKQYRDYRK
                     MF"
     gene            complement(323..1312)
                     /locus_tag="PKW60_14245"
     CDS             complement(323..1312)
                     /locus_tag="PKW60_14245"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPK00642.1"
                     /translation="MGAAEPAEPMTADTGGRLHQTLHYQAMATDFEFTLYAEPRAKST
                     TEVVRIADLAMQAVRDLEEQISQWKPQSQATYINRHAGVEPVRVSFEVFNLLQTCKTV
                     YQETGGAFDVSVGPLLKLWGFYKGEGHLPSPDELRETVEHVGLDKVLLDEAERTVSFS
                     VPGMHLDFGGIGKGLALDAAAEVIRNYGVSSAILHAGTSTVLAIGAPPGTFGWTVRIR
                     NPYNTTEYIDEVCLKDESLSTSASYEKWFELDGKKYCHIVDPRTGLPVEGMISATAIV
                     SSGTLSDALSTAFFVMGAEETQKYCNEHPGVRAILVPHSGSELKPRRIGFDAP"
     gene            1794..>2235
                     /gene="deoC"
                     /locus_tag="PKW60_14250"
     CDS             1794..>2235
                     /gene="deoC"
                     /locus_tag="PKW60_14250"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPK00643.1"
                     /translation="MVSDSPLKPSLRLTQKQLARMIDHTQLRAYATQLDITELCDEAA
                     AFGFASVSINPIWTAYCAKRLAGTAVVVDPTIGFPLGANTARIKIEEAQEAVRHGAQE
                     LDMVINIGALKSGFHDYVEKEIASIVRAVRGIPIKVILETSYLTT"
BASE COUNT          479 a          717 c          589 g          450 t
ORIGIN      
        1 caaacatttt ccggtaatcc cggtactgct tcatattttt gtcgcgcccg gcgacgatcc
       61 agccgccctg caggtgaggc tgatagacgt cgcataccgc gaccacttga atgttcttcg
      121 cgcgcgccag gccgtcacgc agatgcagac agccctgccc gcccgtgccg atactggcta
      181 cacgcacccg atcgttctgg gcatacgaga acggcgaata ccctgacgcg atcatcactc
      241 cagccgccgc gcctgcggta gccatgaaat tgcgcctgga aagtttgtgc ctgctgctgt
      301 atttttcctt catgaccact cctcacggcg catcaaaccc aatgcgcctc ggtttcagtt
      361 cgctgccgct gtgaggcacg agaatggccc gcacaccggg gtgctcattg cagtacttct
      421 gggtttcttc agcgcccatg acgaagaacg ccgtactcag cgcgtcggaa agcgtaccgg
      481 acgacacgat ggcggtcgcg ctgatcatgc cttctaccgg gaggcctgtc cgcggatcca
      541 cgatgtggca atacttctta ccgtccaact caaaccattt ctcgtagctc gcggacgtcg
      601 acagactctc gtctttgagg cagacttcat ctatatactc agtggtatta tacgggttcc
      661 gaatgcgtac tgtccacccg aaagtccccg gcggggcgcc aattgccagc accgtgctcg
      721 tgcccgcatg gaggattgcc gagctaaccc cataatttct aattacttcc gcggcggcgt
      781 ccagggcgag gcctttaccg ataccgccaa aatcgaggtg catgcccggt acggaaaatg
      841 acactgtccg ttcggcctcg tccagcaaga ctttgtccag accgacgtgt tccacggttt
      901 cgcgcaattc gtctggcgaa gggagatggc cctccccttt gtaaaacccc cagagtttga
      961 gcaggggtcc caccgataca tcaaacgctc cgccggtttc ttgatacacc gtcttgcagg
     1021 tctgcaagag gttgaagact tcgaaggaca ccctgaccgg ctccacgccg gcgtgccggt
     1081 tgatgtaggt ggcttggctc tgcggcttcc actggctgat ttgctcttcg aggtcgcgca
     1141 cggcctgcat ggcgaggtct gcaatccgca ccacctccgt cgtgcttttg gcccggggtt
     1201 ccgcatacag cgtgaactcg aaatccgtag ccatcgcctg atagtgcagg gtctgatgaa
     1261 gacgcccgcc agtatccgcc gtcatgggtt ccgcaggctc tgcggccccc acggaaccgg
     1321 attctccatc ggcagaggat tcccgcgcgg catccattga cgtttgcgag ggtacggccg
     1381 cccgtccgga cggcgtgcag cccatgcaac ccattgcgcc gcccaaaagg accgccaacg
     1441 cgcagaaccg cacaccatgc tcccgaaacc tgctcttcat gcccgcaagt ccgctccaca
     1501 accgccaggg gatgtgacgg aattttacca accagtctat ctcgcttcaa ctgcaaattg
     1561 gcgaaaacag accacttgga ttaagcctgc cgggccacac tcgcccgggc tgtgggaaac
     1621 aggccggatt tgcccggata tatgcgccta aaaggctgtc tctcacgcag ggattcgcat
     1681 ccacgccagg cctgggataa cgcagctgag aatttatgtc gggtttcgag ctacaatacc
     1741 acgtctttcg gcacagacaa ggaactcgaa agcgcgaaca caggaagcga atcatggtca
     1801 gcgacagccc cctgaaacct tcgttgcgtc tgacacaaaa acaattggca cgaatgatcg
     1861 accacaccca gttgcgagcc tatgccaccc agctcgacat caccgaattg tgcgatgaag
     1921 cagcggcgtt tggatttgca tccgtatcca tcaaccccat ctggaccgcc tactgcgcca
     1981 aacggctcgc aggcaccgcc gtcgtggtgg atcccaccat tggatttcct cttggagcca
     2041 ataccgcccg gatcaaaatc gaagaagcgc aggaagccgt acggcatggc gcccaggaac
     2101 tcgacatggt catcaacatc ggcgcgctca aatcgggctt tcacgactat gtcgaaaaag
     2161 aaatcgccag catcgtccgg gccgtacggg gcatccccat taaagtcatc ctggaaacca
     2221 gctacctgac cacag
//
LOCUS       DAOPBQ010000570         2231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_38279_length_2231_cov_3.389246, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000570 DAOPBQ010000000
VERSION     DAOPBQ010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38279_length_2231_cov_3.389246"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..>2231
                     /locus_tag="PKW60_14255"
     CDS             159..>2231
                     /locus_tag="PKW60_14255"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HPK00644.1"
                     /translation="MKTHHYHPTLNAVAALALLLAAPGAVFAQPQPIATDSCTAVFED
                     GTLVNLSDAAGRTLAAFDGPLGGFACLHRIDATVTIEEAQTRTSEADTAGWSWRAAEF
                     PALPGTWAQTTVTTEADEIVVRQEAVSPQQDLWGVEWSVAGIPLDRNIIVPGQSGVRL
                     TADTPGSQFTFEYPMTWEAQLVIVEGAGGGFYVWAQDPQAQFKRLIVERRQGGWRLRF
                     TAMAYAPFEECSRLVSPGWRLGTYEGDWRVPARRYRDWMLRTFNPAPVEQQHPSWVQD
                     MRCLVVIKLDLEMLEQLGEILDPSQTILYVPGWRTPGYDRDYPNYSDVYPELKPFIDK
                     AHELGFKVMLHVNYFGVDPLNAHYEQFEPYQVRSPWGPHEKEWWLWTRATPEIKFAYI
                     NPACAAWRDLFTDCMVRLCESSGADALHLDQTLCIYNDYNGRIEGMTMAEGNIALHRQ
                     LREALPEVALSGEGLNEITCAHEAFAQRHVWGLQHVEGTWDRALLETAHPISSYLFRP
                     YTIITGYLGCAPPASGQLYAAWNEAYEHWGVIPTLKLLSNTLVHPNGFMRQFREETAF
                     WFAARPEIALDGPWPDSVAFPYRSADGQEVLRTADHRLLADDREISRTISGVDAAALP
                     GSIPGWQAYDATRLFGLLPDAWYPYFGDPRDLATPHLSALPDEYRPEGLVLEKRLGIL
                     RTAQQGGLV"
BASE COUNT          403 a          758 c          669 g          401 t
ORIGIN      
        1 gaaatgccat gtggtcatta cgtaacatgc ggcccattct gccaagccgc cggcggggat
       61 gcaacccgcc gtcctgaaca gtccttttcc ccgggctttt gctccgacgg cggccctcca
      121 gccgtgcatt ttgtcccgcg cccagccata atgccgtcat gaaaacacat cactaccacc
      181 cgaccctcaa cgcggttgcc gcactggccc tcctgcttgc tgcccccggc gccgtattcg
      241 cccagcccca gcccattgcg acggactcgt gtaccgctgt tttcgaggac ggaacactgg
      301 tcaacctctc cgacgccgct gggcgcacgt tggccgcctt cgacgggccg ttgggcggct
      361 tcgcctgcct gcaccggatc gatgccaccg taaccatcga ggaggcccag acgcgtacct
      421 ccgaggccga caccgcggga tggtcctggc gcgccgctga attcccggcc ctgcccggta
      481 cctgggcgca aaccaccgta accaccgaag cggatgaaat cgttgtccgg caggaagccg
      541 tgtctcctca acaagacctg tggggggtcg aatggagtgt cgccggcatc ccgctcgatc
      601 ggaacatcat cgtgccgggg caatcgggcg tgcggctcac ggcggacacg ccgggcagcc
      661 agtttacatt cgagtatccg atgacgtggg aagcgcaact tgtgatcgtg gaaggcgcgg
      721 gcggcggatt ctatgtctgg gcacaggatc cgcaggctca attcaaacgc ctgatcgtgg
      781 aacggagaca gggcggctgg cgcctccgat tcactgcgat ggcctacgcg ccgttcgaag
      841 agtgcagcag gctcgtgtcg cccgggtggc ggctgggcac gtatgagggc gactggcggg
      901 tcccggcgcg ccgttaccgc gactggatgc tgcggacgtt taaccctgcg ccggttgagc
      961 aacagcaccc gtcgtgggtg caggatatgc gctgcctggt cgtcataaag ctcgacctgg
     1021 agatgctcga acaactcggg gagatactcg acccgtcgca gaccatactc tacgtgcccg
     1081 gctggcgcac tcccgggtac gaccgcgatt atcccaacta cagcgatgtc tatcccgagc
     1141 tgaaaccctt catcgacaag gcccacgagc ttggcttcaa agtcatgctg cacgtcaact
     1201 actttggcgt cgacccgctc aatgcgcact acgaacagtt cgagccttat caggtacgga
     1261 gtccctgggg gccgcacgaa aaagaatggt ggctgtggac ccgcgctacg cccgagatca
     1321 agtttgccta tatcaatccc gcgtgtgcgg cctggagaga cctgttcacc gactgcatgg
     1381 tgcgtctgtg cgagtcgagc ggggccgatg cactccatct cgatcagacc ctgtgcatct
     1441 acaacgatta caacggccgg atcgaaggca tgaccatggc cgagggcaac atcgccctgc
     1501 accggcagct ccgcgaagcc ctgcccgagg tcgcattgag cggcgaaggc ctcaacgaga
     1561 tcacctgcgc ccacgaggcg ttcgcgcaac gccatgtctg gggcctgcag cacgtcgaag
     1621 gcacctggga ccgtgccttg ctcgagaccg cgcatcccat cagctcgtac ctgttcaggc
     1681 cgtacacgat catcacgggc tacctgggct gcgcaccgcc cgcgtccggg cagctctacg
     1741 ccgcctggaa cgaagcctac gagcattggg gtgtgattcc gaccctcaaa ctcctcagca
     1801 atacgctggt tcatcccaat ggcttcatgc gccaattccg ggaagagacc gctttctggt
     1861 ttgcggcccg ccccgaaatt gccctggacg ggccctggcc cgatagcgtc gccttcccgt
     1921 accggtccgc agacggccaa gaggtgctgc gaaccgcgga ccaccgcctc ctggccgatg
     1981 accgcgagat ctcccgtacc atctcggggg tcgacgcggc tgcccttccc ggcagcattc
     2041 ccggttggca ggcctacgac gccacgcgcc tgttcggtct cttacctgac gcctggtatc
     2101 cctatttcgg cgacccgcgc gacctcgcaa cacctcatct gtcggctctg ccggacgaat
     2161 accgcccaga gggcctggtg ctggagaaac gtctgggcat tctcaggacc gcgcagcaag
     2221 gcgggctggt c
//
LOCUS       DAOPBQ010000571         2217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_38621_length_2217_cov_2.879278, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000571 DAOPBQ010000000
VERSION     DAOPBQ010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38621_length_2217_cov_2.879278"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>502)
                     /locus_tag="PKW60_14260"
     CDS             complement(<1..>502)
                     /locus_tag="PKW60_14260"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HPK00645.1"
                     /translation="VVLLTVLVVEHAYETSVEASYVENNLSLFQAETAARSAVAYGYS
                     LLASDLETSSLAGVAGTEFDSLVDVWAQGLPCQQINEAVMQCRIQDEYGKLNLNALIK
                     PTNNEENEYVVEALRILLERRGGDPGLVDAILDWLDLDDTVRSNGAEADYYTTLEPPY
                     GCKNGPF"
     assembly_gap    503..512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(607..1518)
                     /locus_tag="PKW60_14265"
     CDS             complement(607..1518)
                     /locus_tag="PKW60_14265"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPK00646.1"
                     /translation="MRNQGFTLMELILVMAIIVIMTAIVYSSLTTVVNSTAMARAAAE
                     ETRLRQFLARSFETNFRSVYSDRTNELPAFQFIGVNDESSDGPMDSVRFCSTAPLMGG
                     MALPGDLKEVRYEVMDSETSKLGLDFDGTRTAKGEKHPGGSMLESVEMPLIAGNTETL
                     DSLSEAELTELAADPNYEAPHWSVPVRSMDLKYFDGADWLDEWDSLQFGRLPWCVHIR
                     INFAKTEAQLKEEASERYDDIDDPDFELVIPIPAGLGVTEDGRALAGFSGQAARDALE
                     QLEREGLRQEDGAPGIRNNIQRERSNR"
     gene            complement(1525..2064)
                     /locus_tag="PKW60_14270"
     CDS             complement(1525..2064)
                     /locus_tag="PKW60_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK00647.1"
                     /translation="MTDRTHRKKPRRAARAGAGGFTLLEIVVALAVIGGVMFILLETH
                     FRALRLYESARDEVTIRNFLSQAMGRAETEVLAGNLAGDGTFGKRYPDYSYTFEAQQM
                     GEGYVLLYDVLVTVKTPQEQKHEIVFMVYDPRSTGGAATSGTRPGAVGPGSIPGSIPG
                     IDLGRLSQRLGSAAAGSGR"
BASE COUNT          411 a          759 c          601 g          436 t
ORIGIN      
        1 gaacgggccg ttcttgcacc cgtagggagg ctccagtgtc gtgtaatagt cggcctccgc
       61 gccgttgctg cgcactgtgt catccaggtc gagccaatcc aggatggcat ccaccagccc
      121 gggatcgcct ccgcgccgct cgagcagtat gcgcaacgcc tccaccacgt actcgttttc
      181 ctcgttgttg gtgggtttga tcagggcgtt cagattcagc ttgccatact catcctggat
      241 ccggcactgc ataacggcct cattgatctg ctggcaggga agcccctgag cccacacgtc
      301 caccaggctg tcaaattccg ttcccgccac gcccgccagc gaggacgttt ccagatcgct
      361 tgccagaagt gaataaccgt acgccaccgc cgaccgcgcg gcagtttcgg cttgaaataa
      421 cgacagattg ttctccacat agcttgcttc gacgctcgtt tcataggcgt gctcgaccac
      481 cagcaccgta agcagcacca cgnnnnnnnn nnaccatcgg caccggcacc gcgagcaggt
      541 tgtgcagcag cgccacgcct tcgtcgcgcg ggcgcgttcc ggccatcgaa ctgaccctgc
      601 gccagctcac cggttgctcc tctctcgctg aatgttgttc ctgattcccg gcgccccgtc
      661 ttcttgccgc aatccctcgc gttccagctg ctccagggcg tcacgcgcgg cctggcccga
      721 aaaacctgcc agagcgcgtc cgtcttccgt cacgccaagt cccgcgggaa tcgggataac
      781 caattcgaaa tccgggtcat caatatcgtc gtaacgctcc gacgcctcct ctttgagttg
      841 cgcctcggtc tttgcaaagt tgatgcggat gtgcacacac cacggcaggc gcccgaactg
      901 cagcgaatcc cattcatcca gccagtccgc gccatcaaag tacttcagat ccatcgagcg
      961 aaccggcacg ctccagtgag gcgcctcgta attgggatcc gcggccagtt ccgtcaactc
     1021 ggcctcgctc agcgagtcca gagtctcggt gttgcctgcg atgagcggca tttccaccga
     1081 ttccaacatc gagccgcccg gatgcttctc gcctttcgct gtccgggtgc catcgaaatc
     1141 cagacccaat ttgctggtct cgctgtccat gacttcatag cgcacctctt tcaggtcgcc
     1201 cggcaatgcc atccctccca taagcggtgc ggtcgagcag aaccgcaccg aatccatggg
     1261 gccgtcagaa ctctcgtcgt tcacgccgat gaactgaaac gcgggcagtt cgttggtacg
     1321 gtctgaatac acgctccgaa aattggtttc aaagctgcga gccaggaact gccgcagccg
     1381 cgtctcctcg gcggctgccc gcgccattgc ggtcgaattc accaccgtgg tcaatgagga
     1441 atacacgatg gccgtcatga tgacgatgat ggccatcacc aggatcagtt ccatcagcgt
     1501 aaagccctga ttacgcatgc cgtgtcaccg cccagagccc gcggccgcgc tgccgagacg
     1561 ctgcgacagc ctgccaagat cgatgcccgg gatcgacccc ggaatcgagc ctggacccac
     1621 agcgccaggc cgcgtgccgg atgtggccgc cccgccggtc gaacgcggat cgtagaccat
     1681 aaacacaatt tcgtgcttct gttcctgggg agtcttgacc gtgacgagca cgtcgtacag
     1741 cagcacgtat ccctctccca tctgctgcgc ttcgaacgta tacgaatagt cgggataccg
     1801 cttgccgaag gtgccgtcgc cggcgagatt gcccgcgagc acctccgttt ccgcgcgtcc
     1861 catcgcctgc gagagaaaat tccggatcgt cacctcatcc cgcgctgatt cgtacagccg
     1921 cagcgcccga aaatgcgttt ccagcagaat gaacatcacc ccgcctatca cggcgagcgc
     1981 gactacgatc tcgagcagcg tgaatccgcc ggcacccgcc cgcgcggcac gacgcggctt
     2041 ctttcgatgc gtgcggtctg tcaaagcggc tcctcttttc cttcgtacac atgcgcgccg
     2101 cccgtgatcg ggtcccaggc gacggtaaaa taatctctgc tgtcgcgggc tttgaggtag
     2161 atgtggaccg gttcggcgag ccccagcggc gtgacatcca cctcgaccag gccgcgg
//
LOCUS       DAOPBQ010000572         2216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_38640_length_2216_cov_3.753818, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000572 DAOPBQ010000000
VERSION     DAOPBQ010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38640_length_2216_cov_3.753818"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PKW60_14275"
     CDS             <1..302
                     /locus_tag="PKW60_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00648.1"
                     /translation="HIATKADPHAHSVFLVNGAPRVSIATQRTEGADWGLDVYHKVRV
                     VRDTASGDIKVFFNDMDTPIMTATDKTFLHGKVGFGSFDDTGNVDNVVLWGKKKE"
     gene            346..>2216
                     /locus_tag="PKW60_14280"
     CDS             346..>2216
                     /locus_tag="PKW60_14280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK00649.1"
                     /translation="MNCDFCGVRSSVGFCGECKRLLCDDCAEKCENCGALACREHMRE
                     TAYRRLCAACMRERNQQFAASIDEMQRYAYEMANQTEGRAGTFFERLIDVLEEVRRRD
                     RDLQDAQAQLEQRIAQRTQTLQRQLQEKDTTCRSAIAMKEAALQATRAKSEFLTNTSH
                     EFRTPMNGVIALTDLLLETELTATQRHYVETIQRSGRALMSLVGDILDYARIEAGRLT
                     ITPIPFDLEVTITDVVELLVPRAEEKGLALIMRYAPNAPRRLIGDAGRVRQILTNLIG
                     NAIKFTHQGHVLVNAECLGLDEERAILRIAVEDTGIGIPQSRLNGVFRQFVQADDATY
                     QYYGGTGLGLPIAHKLSHMMGGRIGVKSTVGEGSRFRVTLPFALDREAAVAPPDDATD
                     LRGLQVLLADPSQMNRRILHEQLSSWGLHVETVSSAEKALAGMRSVCRKGTPFQLTLI
                     THQPGAMDAIVLGETIKTDPQLRDTVLVMLSRTGLRGEAAAAAEAGFTAYLSGVLRSS
                     EFRDMLRAAWSAHENKLDTGLITRHTVAEAREKGAVEESRSKPYINANILVVDDEPVN
                     QEVAREVLKTLGCRVTVASNGQEGLECYRQDQFDAVLMDCEMPVLDGYAATRAIREW"
BASE COUNT          445 a          710 c          673 g          388 t
ORIGIN      
        1 tgcatattgc gaccaaagcc gatccgcacg ctcattcggt gtttctcgtc aacggcgccc
       61 cccgggtctc gatcgccacg caacgcaccg aaggcgccga ctggggactc gatgtctacc
      121 acaaggtgcg cgtcgtgcgg gatacggcct cgggcgacat caaggtattc ttcaacgaca
      181 tggatacgcc catcatgacc gccacggata agactttcct tcacggcaag gtcggatttg
      241 gctcgtttga cgacaccggc aatgtggata acgtcgttct ttgggggaaa aagaaggagt
      301 aattgcgcaa ccatcgggtc tgaggcctcg ggggaatctg cctgtatgaa ctgtgacttc
      361 tgcggtgtcc ggagttcggt cggcttctgt ggcgaatgca aacgcctgct ctgtgacgat
      421 tgtgcggaga aatgcgaaaa ctgcggcgca ctcgcgtgcc gcgaacatat gcgcgaaacc
      481 gcataccgcc gcctctgcgc cgcatgcatg cgcgagcgga accagcagtt tgcagcctcc
      541 attgatgaga tgcagcgata cgcctacgag atggccaacc agaccgaagg ccgggcgggc
      601 acttttttcg agcgcctcat cgatgtgctt gaagaggtcc gccgccgcga ccgcgacctt
      661 caggatgcgc aggcgcagct cgaacagcgc atcgcccagc gtacccagac cctccagcgc
      721 cagctccagg aaaaagacac cacgtgccgg agcgccatcg ccatgaagga agccgcgctc
      781 caggccacgc gcgccaagag cgaatttctg accaacacca gccatgagtt ccggacgccc
      841 atgaacggcg tcattgcact gaccgacctg ctcctggaaa cggaactcac cgcgacccag
      901 cggcactatg ttgagaccat ccagcgctcc gggcgcgccc tgatgtcact ggttggcgac
      961 atccttgatt atgcgcgcat tgaagcgggg cgcctcacca ttacccccat tccctttgac
     1021 ctcgaggtca ccattacgga tgtggtcgag ctgctggtcc cgcgcgccga ggaaaagggg
     1081 ctcgccttga tcatgcgcta cgccccgaac gccccccggc gcctcatcgg cgatgcgggc
     1141 cgggtccggc agattttgac caatctcatc ggcaatgcga ttaaattcac gcaccagggc
     1201 catgtgctcg tgaacgcgga gtgcctcgga ctggatgaag agcgcgccat tctgcgcatc
     1261 gccgtggaag ataccggtat cggcatcccg caatcgcggc tgaacggcgt cttccgccag
     1321 tttgtgcagg ccgatgacgc cacctatcag tattacggcg gcaccggcct gggcctgccc
     1381 atcgctcaca aactcagcca catgatgggc gggcgtatcg gcgtgaagag cacggtcggc
     1441 gaaggctcgc ggtttcgcgt cacgctcccg ttcgcgctgg atcgcgaagc cgcggtggcg
     1501 ccgccggacg acgcaaccga cctgcgcggg ttgcaggtgc tgctggccga tcccagccaa
     1561 atgaaccggc ggattctcca cgaacagctt tccagctggg gtctgcacgt cgagacggtc
     1621 tcgagcgcag aaaaagcgct cgccgggatg cgcagcgtct gccgcaaggg caccccgttt
     1681 caactgaccc tcatcactca tcagccgggc gccatggatg cgatcgtgct gggtgaaacc
     1741 atcaagacgg acccccagct gcgcgatacc gtgctcgtga tgctgtcgcg cacaggcctg
     1801 cgcggggagg cggccgcggc tgccgaagcc ggattcaccg cctatctcag cggggttctt
     1861 cgaagcagcg aattccgtga catgctccgc gccgcctggt ctgctcatga aaacaagctg
     1921 gataccggtt tgattacgcg gcacacggtt gcggaggccc gtgaaaaagg cgctgtggag
     1981 gaatcgcgct ccaaaccata catcaacgcc aacatcctgg tggtcgatga cgagccggtg
     2041 aaccaggagg tcgcccgcga agtcctcaag accctgggat gccgcgtcac cgtggcatcc
     2101 aacggccagg aaggcctcga atgctaccgc caggaccagt tcgacgccgt gctgatggat
     2161 tgtgagatgc cagtgctcga cgggtatgcc gccacccgcg ccattcgcga atggga
//
LOCUS       DAOPBQ010000573         2216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_38642_length_2216_cov_3.415548, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000573 DAOPBQ010000000
VERSION     DAOPBQ010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38642_length_2216_cov_3.415548"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PKW60_14285"
     CDS             complement(<1..555)
                     /locus_tag="PKW60_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA small subunit methyltransferase 1"
                     /protein_id="HPK00650.1"
                     /translation="MAALYLVATPIGHLEDMTYRAVRVLNEIEALACEDTRRTRVLLE
                     HYGIPKPRTLFSYHEHNELAAGKRILGLLDAGVSVAVCSNAGFPGVSDPGFRIVSGAI
                     EAGHDVIALPGASAVHVALVQSGLPTSSYVFKGFPPRKSGARRRFLALERDLPHTLVL
                     YESPFRIGALLADAHAELGNRRAAV"
     gene            complement(575..1678)
                     /gene="corA"
                     /locus_tag="PKW60_14290"
     CDS             complement(575..1678)
                     /gene="corA"
                     /locus_tag="PKW60_14290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPK00651.1"
                     /translation="MKHAAEAPRGAVTTDLNGLKPELQLIAYGPAGFYESSEPDLEAI
                     PALRKKWPVLWLHVGGLGDAEVLAAIGRTFDIHRLALEDVLAMNQRAKIEDYDKRLFV
                     VMRILEPGPTVRTGQLSIFVGDGFAVTFQEHPVGCLDPIRKRIREGLGPLRTMAADYL
                     AYSIIDAAVDAYFPYLDDFSEKLGELEDDALYRPTPEIAARIHEAKRDLLALRRAMAP
                     MREGLGALVLGGAGHISETTRLHLRDCYDHCIMLLEMMESYRELTGSLLDVYLSSVGN
                     RMNEIMKMLTLISTIFIPLSFLTGLYGMNFDHHVSPWNMPELHTRYGYPIFLGVVLAI
                     GIFQLLLYWKRGWFGGVRGKPVVPPEERDRDQH"
     gene            complement(1901..>2216)
                     /locus_tag="PKW60_14295"
     CDS             complement(1901..>2216)
                     /locus_tag="PKW60_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00652.1"
                     /translation="EEADRTMEEATKEEDFETAKRTYQKAQAVLKYVPDSQSAAHVAS
                     RDENIARAKNKFDQVDAEKAKWVEDQERRKKMEEDRRAAEQAAALAGAAAAPTAPVAP
                     AP"
BASE COUNT          421 a          718 c          647 g          430 t
ORIGIN      
        1 cacggccgcg cgccggtttc ccagttcggc gtgcgcatcg gcgagaagcg cgccgatgcg
       61 gaatggcgac tcgtagagca ccagcgtgtg gggcaggtcg cgttccaggg cgagaaaacg
      121 gcgccgcgca ccgctcttgc gcggcgggaa ccctttgaac acataactgg acgtaggcag
      181 acccgattgc accagcgcca catgaaccgc gctcgcgccc ggcagcgcga tcacgtcgtg
      241 ccccgcctcg attgccccgg agacgatccg gaacccggga tcgctcacgc cgggaaaccc
      301 cgcattcgaa caaacggcca ccgagactcc ggcgtcgagc aggccgagaa tgcgcttgcc
      361 cgcggccagt tcattgtgtt cgtgatacga aaaaagcgtc ctgggcttgg gaatgccata
      421 atgctcgagc agcacgcggg tccggcgcgt gtcctcgcag gccagcgctt cgatctcatt
      481 caagacccgc acggcgcgat acgtcatgtc ttccagatgg ccgatggggg tagctaccag
      541 atacaacgcc gccatgaatg cctcctgccg cgcctcagtg ctggtcccgg tcgcgttctt
      601 ccggcggcac aacgggcttc ccgcgaacgc cgccaaacca tccgcgtttc caatagagca
      661 gcagctgaaa gatgccgatc gccagcacca cgcccagaaa gatcgggtat ccataccgcg
      721 tgtgcagttc gggcatgttc cacggcgaca cgtgatgatc gaagttcatc ccatacagcc
      781 ccgtcagaaa actcaagggg atgaagatgg tcgagatgag cgtcagcatt ttcataattt
      841 cattcatgcg gttgcccaca ctcgacagat acacatcaag caggcttcct gtcaattccc
      901 ggtacgattc catcatttcc agcagcataa tgcaatggtc gtagcagtcc cggaggtgaa
      961 gccgggtcgt ttcggagatg tgaccggccc ctcccagtac gagcgcgccc aatccctcgc
     1021 gcatcggcgc catcgcccgg cgcaacgcca gcaagtcgcg cttcgcctcg tgaatacgtg
     1081 ctgcaatctc aggtgttggc cggtacaacg catcgtcttc cagctccccg agcttctcgc
     1141 tgaaatcgtc caggtacggg aaataggcgt ccacggcggc atcgatgatg ctgtaggcca
     1201 gataatcggc tgccatggtg cgtaacggac ccagcccctc ccggatgcgt tttcggatgg
     1261 ggtcgaggca tcccactgga tgctcctgaa acgtcactgc gaatccgtcg cccacgaaga
     1321 tactcaattg gccggtacgg accgtcgggc ccggctccag tatgcgcatc accacaaaca
     1381 gccgcttgtc gtagtcttcg attttcgccc gctgattcat cgcgagcaca tcttcgagag
     1441 ccagacgatg gatgtcgaac gtgcgcccga tcgccgcaag cacctccgca tcgcccagac
     1501 cgcccacgtg cagccacagt acgggccact ttttcctgag cgccgggatc gcctcgagat
     1561 cgggttcgga ggattcgtaa aagccggcgg gcccataagc gataagctgg agctccggtt
     1621 tcaggccgtt caggtcagtc gtgacagcgc ctcgcggtgc ttccgcagcg tgtttcacgc
     1681 gcccttttcg tggccctttg gtactcaacg ccatcgcccg ctccttttct tcgccgggca
     1741 cagtcagacc cgcggcctcc agtataatcg agaaccgcaa accagggaca aggagttgaa
     1801 ttgcccagct ggcatggagc ggagccaggg acgcgcggtg ccgatgatgc ggggcatggc
     1861 cacgcgagcc atgccccgtt tacatttcct ccacggaatg ctagggcgcc ggcgcaacgg
     1921 gagcggtggg cgcggcagca gcgccagcaa gagcagcggc ctgttccgca gcccggcggt
     1981 cttcttccat ctttttgcgc cgctcctgat cctccaccca tttcgccttt tcggcatcga
     2041 cctgatcgaa tttattcttg gcccgggcga tgttttcgtc gcgcgatgct acgtgggcgg
     2101 cggactggct gtcgggaaca tacttgagaa ccgcctgggc cttctggtag gtccgtttgg
     2161 cggtctcgaa atcctcttct ttggtggctt cttccatagt gcgatcagct tcttcg
//
LOCUS       DAOPBQ010000574         2201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_38996_length_2201_cov_3.289376, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000574 DAOPBQ010000000
VERSION     DAOPBQ010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38996_length_2201_cov_3.289376"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="PKW60_14300"
     CDS             complement(<1..1735)
                     /locus_tag="PKW60_14300"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00653.1"
                     /translation="MRLLKCLGLVGVFALAAWQAGGELVDHWGLCPASPAVVNPVTTS
                     PLQEVISLRGEWDFVVDPRLMGRHRMGKGPGWNEPDWNGVRKIQVPGCWEAQGVGEPG
                     MSLPWDLPFDCIPRPLNHVYMGTARYRRTVEIPADWAGKRIWLKVGGVRSEAWFWVNQ
                     QRVAHLNTYCGTYKYDITDLVAAGAAAEIVATVRNDSPSRKGCMAAFHRFGGIYRDIE
                     LEATPSTWVDDVWVRGDIDQKTAQVHVSIRSADKADASALALKIGITTLDGTHAGSLT
                     EQIMLDENGGADRVCEVPLSVLRPWTPETPNLYLADVTLISGETPVHGWTERFGIKKL
                     EVRGEQFYLNGQPYFLRGYGDDYVYPMTLISPPDTEEHRQHLAIAHRAGFNYVRHHTH
                     CEIPEFFEAADEFGVMIQPELPYYHDITTEGFEFDPLRDIQELYRHFRRYVSFASYST
                     GNEGHLGSPLDIAVYQWAKSTDPDRIFQHQDGGANTPENSDYYSPNGYGLASSIVPWA
                     PGTFEDLGVPFISHEYLNLGIKMDPRIAPKFTGAIPAPRSLEAYEESLQAAGLDRAWG
                     DACLDAAHGLQA"
     gene            complement(1921..>2201)
                     /locus_tag="PKW60_14305"
     CDS             complement(1921..>2201)
                     /locus_tag="PKW60_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00654.1"
                     /translation="YLFADGGRAEWVTGNSVAGWLEPGSVRVVNADGVYIEFTVRHGG
                     VVQLRKRTADVSPAKMPSGGISGDLLAVLGVGGFLLLRKRMSRRVSAN"
BASE COUNT          423 a          733 c          621 g          424 t
ORIGIN      
        1 aggcctggag tccgtgagcc gcgtcgagac aggcatcgcc ccaggcacgg tccagcccgg
       61 ccgcctgcag cgattcctca taggcctcca ggctgcgtgg cgcgggtatc gctcccgtga
      121 atttcggcgc aatgcgcgga tccattttga ttccgagatt caggtactcg tgagaaatga
      181 acgggacgcc caagtcttca aaggttcccg gcgcccacgg gacgatagat gacgccagtc
      241 cgtagccatt gggcgaatag taatcggaat tctccggcgt attggcgccg ccgtcctgat
      301 gttgaaaaat gcggtccgga tccgtgcttt tggcccactg gtagacagca atgtccagcg
      361 ggctgcccaa atgcccctca ttcccggtcg aatacgaggc aaaagacaca tagcgccgga
      421 aatgccggta cagctcctga atatcgcgca gggggtcgaa ttcaaaccct tccgtcgtga
      481 tgtcgtggta atacggcaat tccggctgaa tcatcacgcc gaattcgtcg gcggcctcga
      541 aaaactccgg gatctcgcag tgggtgtgat gccggacata attgaacccg gcccggtgcg
      601 caatggccag atgctggcgg tgttcctccg tgtctggcgg ggaaatcagc gtcatcgggt
      661 agacgtaatc gtcgccatac ccccgcagaa aatagggttg cccgttgaga taaaactgtt
      721 cgccccgcac ctcgagcttt ttgatcccaa accgctcggt ccagccgtgc acgggggtct
      781 cgccggaaat cagcgtcaca tcggccaggt agaggttcgg cgtctcaggg gtccacggcc
      841 gcaggaccga caacggcacc tcgcagaccc tatccgcgcc gccgttctca tccagcatta
      901 tctgttctgt cagcgacccc gcgtgcgtgc cgtccagggt tgtaatgccg attttgaggg
      961 cgagcgcgct ggcatcggct ttgtccgcgc tgcgtatgct gacgtgcacc tgggccgttt
     1021 tctggtcgat atcgccccgc acccagacat cgtccaccca ggtggacggc gtagcctcca
     1081 gctcaatatc acgatagatc ccgccaaacc ggtgaaacgc ggccatgcac cccttccggc
     1141 tgggcgaatc gttgcggacg gtcgccacaa tttccgcggc agcgcccgct gcgaccaggt
     1201 cggtgatgtc gtacttatac gtgccgcaat aggtgttcag atgggcgacg cgctgctgat
     1261 tcacccagaa ccaggcctcc gaccgcaccc cgcccacttt cagccagatc cgttttcctg
     1321 cccagtccgc tgggatctcg accgtgcgcc ggtaccgcgc cgtgcccata tatacgtggt
     1381 tcaggggccg gggaatacaa tcgaacggca gatcccacgg taagctcatc ccgggttcac
     1441 caactccctg cgcctcccag catccgggca cctggatctt ccggacgccg ttccaatcgg
     1501 gctcattcca tcccggccct ttccccatgc ggtggcggcc catcaggcgc ggatccacta
     1561 cgaagtccca ttccccgcgt aacgagatga cttcctggag aggtgatgtt gtcacggggt
     1621 tcaccacggc tggtgacgcc gggcaaagcc cccaatggtc caccagctcg cctcccgcct
     1681 gccaggctgc caacgcgaaa actcccacaa ggcccaggca tttcaataga cgcattgccg
     1741 catttctcct gctttttcaa gaaacggtcc cggatctgtt tcgagtatac acaggcgggt
     1801 acgggatagc atcccgcagc ttccgcagcg tctcgtgaac aaaagggtcc gtggcatcgc
     1861 tgacgccatg ccacggaccc cattccctct cgatctgccg acgattaact ttttcggccg
     1921 ctaattggcg ctgacccggc ggctcatccg tttgcggagc agcagaaaac cgcccacgcc
     1981 caacacggcc agcaggtcgc cgctgatgcc tccggacggc atcttcgcag gcgagacgtc
     2041 cgcggtgcgt ttacgcaatt gcaccacgcc accgtgccga accgtgaact cgatgtagac
     2101 gccgtcggcg ttcacaacgc gtacggagcc cggctcaagc catccggcca cgctgttacc
     2161 cgtgacccat tccgcccggc caccgtctgc aaagagatac g
//
LOCUS       DAOPBQ010000575         2201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_39000_length_2201_cov_3.040075, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000575 DAOPBQ010000000
VERSION     DAOPBQ010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39000_length_2201_cov_3.040075"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="PKW60_14310"
     CDS             <1..1606
                     /locus_tag="PKW60_14310"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HPK00655.1"
                     /translation="WFAGGIAADSASGPENLLANPGFEAGTQGWFIYTEGGAQASVAH
                     EVESVNGSSGAVRIGCQASGAKPHHIQFYTNGIAIERGGVYRLTMAMRASEPCEISPP
                     VLMKNSAPWMRYANPRKPHNISVTSEWAEHTLVYGATATAADGRLNFMLGGAVPAGVS
                     LYLDQIGLERLGEGLIPRDVGNIVFNHGPHCGVKVWGREDLRQQDDYWYDEDGFGVYV
                     YSAENPAERYESIECALRDHIIYQQNQHHILYENLACLYGAAHGIGGGSTHNIIVRDC
                     DFGYIGGGDQMGDGRRVRFGNGVEFWGPAHDCLVERCRFWEIYDAAMTHQSSGDAAQY
                     RIVYQNNLVWNCEYSFEYWNRPETSTTHHIWFINNTCLNAGHGWGHTQRPDPGGRHLC
                     FYASPAQQTEFYIGNNIFYEAKTNAFYAPSWPVEQVKSLIMDHNCWYQAEGVMAALSE
                     GSFTMAEFDACRTLTGLDAHSIVAQPGLVDAKALDFRLAPDSCCIDTGREIKALYPRS
                     ADFAGTPVPQGKAPDIGAYEFPAPTN"
     gene            complement(1637..>2201)
                     /locus_tag="PKW60_14315"
     CDS             complement(1637..>2201)
                     /locus_tag="PKW60_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPK00656.1"
                     /translation="EQKKSIAAGMDAHFVKPVEPAMLMELLMRWLHKQPAGVPEGSTM
                     DDVTHKPAENKSTAQAPQLIDDTIPVLDTQRAAEITGGNVKILKRVTGVFLESLPQEV
                     EALAAALTGGQRDTVQHKAHALKSAAASIGGLRVNRIALELEAAAKDDRLAGVSEHYA
                     NELVAEFAQLKAALEAVDWEKEVAPAP"
BASE COUNT          408 a          658 c          697 g          438 t
ORIGIN      
        1 gtggttcgcg ggcgggatag cagcggacag cgcttcgggc ccggaaaacc tgctcgcaaa
       61 tcccggtttc gaggcgggca cacagggctg gtttatctac acggaaggcg gcgcgcaagc
      121 ttcagtggcc catgaggtgg aaagcgttaa tggctcatcc ggcgcggtgc gcatcggctg
      181 ccaggcttca ggcgcgaaac cgcatcatat ccaattttat acgaacggga tcgcgatcga
      241 acgcggcggt gtgtaccggc tgacgatggc catgcgggct tccgagccgt gcgagatctc
      301 tccgcccgta ctcatgaaga acagcgctcc gtggatgcgc tatgccaacc cgcgcaagcc
      361 gcacaacatc tccgttacct ccgaatgggc cgaacatacg ctcgtctacg gcgccaccgc
      421 gaccgcagcg gacgggcgtc tcaatttcat gcttgggggc gctgtgcccg ccggtgtgtc
      481 actgtatctg gaccagatcg gtctggagcg cctgggtgaa ggcctgattc cacgcgacgt
      541 gggcaacatc gtgttcaacc acggccccca ctgcggagtt aaggtctggg gccgcgaaga
      601 ccttcgacag caggacgact actggtacga cgaggacgga ttcggagtct atgtatattc
      661 ggcggaaaat cctgcggaac ggtacgaatc catcgaatgc gcgctgcgcg atcacatcat
      721 ttaccagcag aaccagcacc acatactgta cgagaacctg gcctgtctgt acggtgcggc
      781 gcatggcatc ggcgggggca gcacgcacaa catcatcgtg cgggattgcg atttcggcta
      841 catcggcggc ggcgaccaga tgggggacgg ccgcagggtc cggttcggga acggagtgga
      901 gttctggggt ccggcccacg actgcctggt agagcggtgc cggttctggg agatctacga
      961 tgccgccatg acccatcaaa gcagcggaga cgctgcccaa taccgcatcg tctaccaaaa
     1021 caatctggtc tggaactgcg agtattcgtt tgaatactgg aaccgtcccg agacctcgac
     1081 gacccatcac atctggttta tcaacaacac ctgcctgaac gccgggcacg gctggggcca
     1141 tacccagcgg cctgatcccg gcgggcgcca cctgtgtttt tatgcgagcc cggcacagca
     1201 gaccgagttt tacatcggga acaacatctt ttacgaggcg aaaacgaacg ccttctacgc
     1261 gccaagctgg cctgtcgaac aagtgaaaag cctcatcatg gatcacaatt gctggtacca
     1321 ggcggaaggc gtgatggcgg cgctgtctga gggctcattc acgatggccg agtttgatgc
     1381 ctgccgaacc ctgacgggcc ttgatgcgca ctccatcgtc gcgcaacccg gcctcgttga
     1441 tgccaaggcg ctggactttc ggctggcgcc cgattcgtgc tgcattgata ccggcagaga
     1501 gatcaaggcc ctgtatccga gatccgcgga ttttgcaggg acccccgtcc ctcagggaaa
     1561 ggcgcccgac atcggagcgt acgagttccc ggcgcccacg aactgacagg cattgggccg
     1621 tcacgcggtt accgcgttac ggcgccggtg cgacttcttt ctcccagtcg acggcttcca
     1681 gggcggcttt cagctgggcg aattccgcca caagctcgtt cgcatagtgt tctgagaccc
     1741 cggccaggcg gtcgtccttg gcggcggcct cgagctcgag cgcgatccgg ttgacgcgaa
     1801 ggccgccaat ggaggccgcg gcgctcttaa gggcgtgggc cttgtgctgg accgtgtcgc
     1861 gctgtccgcc tgtgagggca gccgcgaggg cttccacttc ctggggcagg ctctcgagaa
     1921 acacgcccgt gacccgtttg agtatcttga cgttgccgcc ggtgatttcc gcggcgcgct
     1981 gtgtgtccag cacggggatc gtgtcgtcaa tgagttgcgg cgcctgtgcc gtggatttgt
     2041 tttctgctgg cttgtgcgtg acgtcgtcca tggtgcttcc ttcgggcact cctgcaggtt
     2101 gtttgtggag ccagcgcatc agaagttcca tcagcatggc gggttccacg ggtttcacaa
     2161 aatgggcatc catgccggct gcgatggatt ttttctgttc g
//
LOCUS       DAOPBQ010000576         2199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_39042_length_2199_cov_3.234608, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000576 DAOPBQ010000000
VERSION     DAOPBQ010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39042_length_2199_cov_3.234608"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..515
                     /locus_tag="PKW60_14320"
     CDS             258..515
                     /locus_tag="PKW60_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00657.1"
                     /translation="MAAAKAGVEKLLALAGQFIATQKGVWEHEDWEAFLAKAGALGMP
                     MDDECKRNLGNILEAGKFFYGILPSTPAPKKAPAAKKAPKK"
     gene            749..>2199
                     /locus_tag="PKW60_14325"
     CDS             749..>2199
                     /locus_tag="PKW60_14325"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HPK00658.1"
                     /translation="MVIVLLGIVLAILAALVCGFLAFIRTFSIQNRLNRLELELQRLA
                     QRAGHPSAPPLERPASSLTAEKETSLAAAAHGTAPEPPVYMARSRQDAGPAGAVKPQA
                     APGPRLPENLRTLTGNLSWEMLAGTKGLLWAGVLFVIVGVALFLNYLYSLGFIDEHVR
                     LLIAVAGGALALGLGMWFRKKGWEVISQGFSGLGIAIFYVCVYFSFHIYAFTGQAVSF
                     TLAAGVTVLAVVLAVVQHAPSIAVLAILGGFLSPILISTGENHPYSLFSYITLLNVVA
                     VGVAWFKRWRFLDVLCFAGTALIYSAWYLKFFGGYGEPDMTVPAALYAATFYILFFGV
                     ATIYPLVRQVKSRPQDLVLAATNAVFWFFAFYNVLYAHHRYTLGAIVLGQALLAVLVM
                     LAWRRRVAGDAAAQGVLLVLAMGLAILAVPILLRLYAYQVTWAVQGFVFVYLALKYRH
                     GLAAGSGITAWTLGGLALYLTLLSIPCGSFRYST"
BASE COUNT          393 a          671 c          640 g          495 t
ORIGIN      
        1 gtcacttgac gcgtacgtgc ggctgcaagt tcgataagac cgcgcccccc ggccacgtcc
       61 ccgcggcgct gtagaggcgt ccatcagcag ctgaatggga tgacaggtat ccacatcagt
      121 gggcattttc tgctcactga agtcccggcg ctgcagtccc gggaaatctt cgtgcctgct
      181 ttttggttgt gttatactgc caactgctgg gatttttggg aatgcccgca gacaatgtcc
      241 gcaggaagga ggtagccatg gccgccgcaa aagctggcgt cgaaaagctg ctcgcactcg
      301 ccgggcagtt cattgcaaca caaaaagggg tctgggagca cgaagactgg gaagccttcc
      361 tggcgaaagc cggagcgctg ggcatgccca tggacgacga gtgcaaaaga aatctgggga
      421 acatcctgga ggcaggcaaa ttcttttacg gaatcctacc gtcgaccccg gcccccaaaa
      481 aagctcccgc ggcaaagaaa gccccaaaga aatagccaac ctgctcgcat tgccgcgatc
      541 aaacgaagtc cggttatggc ctcgcgtgac cttgttgagg cacgctctgc tgtcgaaatc
      601 tgacgacctt gcgcagccca ttctattgag ccagtacgcg tgtccactat atgctgaatt
      661 cgcacaggat gccggctctc cgccctgagc gtacggaaag tctacgcagt ccgctcctgc
      721 aattacgcgt tcgcaaaaag gggctaccat ggtaattgtg cttctgggga tcgtcctggc
      781 gattctggcc gcactggtat gcggattcct cgccttcatc cgcaccttca gcatccagaa
      841 ccggctcaac cggctcgagc tggagctgca gcgcctcgcc cagcgcgccg ggcatccctc
      901 cgcgcctcct ctggaacgcc ccgcatcctc attgacggca gaaaaagaaa cctccctggc
      961 ggcggctgcc cacggcaccg cccccgaacc accggtatac atggcccgct cccggcagga
     1021 tgccgggcca gccggcgcag taaagccgca agcagcgccc gggcccagac tcccggagaa
     1081 ccttcgcacc cttaccggca acctttcctg ggaaatgctg gcagggacaa aaggcctgct
     1141 ctgggccggc gtgcttttcg tgatcgtcgg cgtggcgctc tttctcaact acctgtacag
     1201 cctgggtttc atcgacgagc atgtgcggct gctgatcgcg gtggccgggg gcgcgctcgc
     1261 gctgggtctc ggcatgtggt ttcgtaaaaa gggctgggaa gtcatctcgc agggcttttc
     1321 cggcctgggt attgccattt tttacgtgtg cgtctatttc tcgtttcata tctatgcgtt
     1381 caccgggcag gctgtatcgt tcacgctggc agccggagtg acggtgctgg ccgtggtatt
     1441 ggcggtagtt cagcacgctc cctcaattgc ggtgctggct attcttggcg gatttttgag
     1501 tcccattctc atttcgacgg gtgaaaacca cccctacagc ctgttcagct acatcacgtt
     1561 actcaacgtg gtcgcggtgg gagtagcctg gttcaaacgc tggcgctttc tcgacgtcct
     1621 gtgttttgcg ggcaccgcgc tgatttacag tgcatggtat ctgaagttct tcggaggata
     1681 cggcgaacct gacatgacgg ttcccgcagc cctgtatgcc gcgacattct atatcctgtt
     1741 tttcggtgtt gcgacgattt atccgctggt gcggcaggtg aaatcacgcc cccaggacct
     1801 ggttctggcg gcaacgaacg cggtgttctg gttttttgcc ttttataacg tcctttacgc
     1861 ccaccaccgg tatacgcttg gggccatcgt acttggacag gctcttctcg ccgtgctggt
     1921 catgctggcg tggcggaggc gcgttgccgg agacgccgcc gcacagggcg tcctgctcgt
     1981 tctggccatg ggccttgcga ttctggccgt tcccatcctg ttgaggctct acgcctatca
     2041 ggtcacctgg gccgtccagg ggttcgtgtt cgtgtatctg gctctgaagt accggcacgg
     2101 acttgccgca ggcagcggga ttaccgcgtg gacgctcggc ggactcgcgc tgtacctcac
     2161 cctcctctcc ataccgtgcg gttcattccg gtattcaac
//
LOCUS       DAOPBQ010000577         2197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_39101_length_2197_cov_3.612979, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000577 DAOPBQ010000000
VERSION     DAOPBQ010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39101_length_2197_cov_3.612979"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1917)
                     /locus_tag="PKW60_14330"
     CDS             complement(<1..1917)
                     /locus_tag="PKW60_14330"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPK00659.1"
                     /translation="MKHSVILMAVLTGLVWGASAQVFVDFQASVTLGRAPLDVSFTDN
                     SSTGGATVTGWLWDFGDGATSTEQNPSHTYTEPGNYAVTLKITVGGSQYPRTRPRCIN
                     VPNFAAPDVGQIDLSDAVVVVNPGDPPAAEAKAPEVLAEEVLKRSGLDWPITTAMPGT
                     ATVIAITSGRGTATAPEGYTLFTENAGLRTIVWVLGNDSRGALFGTGALLRALECRPG
                     SVVLPEPINLTTAPVYPIRGHQLGYRNTNNTCDAWDKAAYEQYIRELVIFGTNTIENV
                     YALSPGASPHFTTSEEQMHIHLSTICADYGLDYSLWAPVETTLPGSAATELAAQEALY
                     KKLPRLDAVFVPGGDPGNNDPLEVMRYLEDLATLLRSYFPDAELWVSNQGFEPEENDA
                     FFDFLQTRQPSYLTGVVYGPWTKLSIAEERNRTPLNYPIRLYPDLTHNVRCQFPYKNI
                     DRAFAHVLGREAINPSPIASIIEHDTHAPETMGFVGYSDGVHDDVNKCVWTQRAWDPA
                     ASAEAILIDYARFFFGPDSAPAVKDGILALEDNWSHPAATNTGINDTFAAWNALETAY
                     PDLQNNWRWNLCLVRAYYDMYLRYRVIDEAALEQQAYDQLAQAPALGSAAAIANARAE
                     LLKAEALSPVQQARR"
BASE COUNT          411 a          616 c          720 g          450 t
ORIGIN      
        1 gcggcgggcc tgttggacag gggacaaagc ctcggctttc agaagttccg cgcgcgcgtt
       61 agcgatggcg gccgcggaac ccagggcggg cgcctgcgcg agctggtcat aggcttgctg
      121 ctcgagcgcg gcctcgtcga tcacacgata tctcaagtac atatcgtaat atgcacgcac
      181 gaggcagagg ttccagcgcc agttgttttg cagatccggg taggctgttt ccagcgcatt
      241 ccaggcggca aatgtgtcgt tgatgccggt attggttgca gctggatgcg accagttgtc
      301 ttccagcgcg aggatgccgt ccttaacggc cggggcggaa tccggcccga agaaaaagcg
      361 cgcgtagtcg atcagaatgg cctcggcgga cgcagcggga tcccaggccc gctgggtcca
      421 cacgcatttg ttgacgtcat catggacgcc gtcggaatat cccacaaatc ccatggtctc
      481 gggggcgtgg gtatcgtgct cgatgatgct tgcaatggga ctcggattaa tcgcttcgcg
      541 gccgagtacg tgggcgaaag cgcggtcgat atttttgtat ggaaattggc agcgcacgtt
      601 gtgcgtcaga tcgggataga gccggatggg ataattgagt ggcgtgcggt tgcgctcttc
      661 ggcgatgctg agcttggtcc acggaccgta gactacgccg gtgagatagc tgggctgacg
      721 ggtctggaga aaatcgaaga aggcgtcgtt ttcttcgggt tcaaacccct ggttggaaac
      781 ccagagctcg gcgtccggga aataagaacg cagcagcgtg gccaagtctt caaggtaacg
      841 cataacctcc agcgggtcat tgttgcctgg atcgcctcca ggcacgaaga ctgcatccaa
      901 tcgcggcaat ttcttgtata gagcctcctg agcagccagt tccgtggccg cactgccggg
      961 aagcgtggtt tccacagggg cccagaggct gtaatccagg ccatagtccg cgcagatggt
     1021 gctcaaatgg atgtgcatct gctcctcgct ggtggtgaag tgcgggctgg cgccggggct
     1081 caacgcgtag acgttttcta tggtatttgt gccaaatatg acaagttcgc ggatgtactg
     1141 ttcgtaggcg gccttgtccc atgcgtcgca agtgttattc gtgttgcggt agccgagctg
     1201 gtgcccacgg atggggtaga ccggagcggt cgtaaggttg atgggttccg gcagcacgac
     1261 gctgccgggg cggcattcca gggcgcgcag cagcgctccc gtgccgaaca gggcgccgcg
     1321 cgaatcgttc cccagcaccc agacgatcgt ccgcagaccg gcattttcgg tgaaaagcgt
     1381 gtagccttcg ggggcagtgg cagtgcctcg cccggatgtt atggcaatca ccgtggcagt
     1441 gccgggcatg gcggttgtta tgggccagtc cagcccgctg cgtttgagta cctcttcggc
     1501 caaaacctcc ggggctttcg cttcggccgc tgggggatca cccggattca cgactaccac
     1561 ggcgtcactc agatcgatct ggccaacgtc cggtgcggcg aaattgggca cgttgatgca
     1621 gcggggccgg gtcctgggat attgcgagcc gcccacggta atcttgagcg ttacggcata
     1681 gtttcccggc tccgtgtacg tgtgcgacgg attctgctct gtacttgtcg cgccgtcccc
     1741 gaaatcccag agccagcccg tgacagtggc gccgcctgtc gaggagttgt ctgtaaatga
     1801 cacatccaaa ggcgcgcggc caagtgtgac gctggcctgg aaatccacga aaacctgggc
     1861 gctggcgccc cacacaagtc cggtcagaac ggccatgagt atgacggagt gcttcatgat
     1921 gccccttcga gtggcgtcgg gcccggccgc cgaatctggc gcaggcacgc gcggttgcgt
     1981 cagctgcgtt ttcgcctacc cccccgcccg catacgcctg tggccagagg cgctgcggcc
     2041 tgcatggata gtagataggg gacgagagcg aattcaagcg caacgagagg cagtcaacgg
     2101 cgattgctgc aggctcacgt cagaaaatat cccatcgggt gaacccctgt caagccattt
     2161 gctaatctta ccggggcgta tatcgtcgcg ccatttg
//
LOCUS       DAOPBQ010000578         2182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_39471_length_2182_cov_3.772449, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000578 DAOPBQ010000000
VERSION     DAOPBQ010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39471_length_2182_cov_3.772449"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="PKW60_14335"
     CDS             <1..893
                     /locus_tag="PKW60_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPK00660.1"
                     /translation="VAPLLSRSQVFELKPLDDEHVVDLLKRVLTHPERGMAELEIEVD
                     EPALRHFARYAEGDARRALNGLEVAMLTTPPVDGRIHITLDIAQESIQRKLIHYDGTG
                     DQHYDAASAFIKSMRGTDPDSALYWMAYMLEAGEDPRFIARRICIAAAEDVGNADPQA
                     LVLATAAWQACEFIGLPEARIILAQAATYVACAPKSNAAYLGIDAALGDVREGKTVAV
                     PRHLQDAHYRGAQRLGRGAGYQYAHNDEGGFVVQEYGVPRWTYYRPAGRGKEGEFKQR
                     LEECARREEAQENEPGTPGE"
     gene            complement(912..1679)
                     /locus_tag="PKW60_14340"
     CDS             complement(912..1679)
                     /locus_tag="PKW60_14340"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPK00661.1"
                     /translation="MLRRIAVVTTDEKWFGEISAAAENSDTQCVQVAPGTPLPPASAG
                     LIAPDAARHPNEVLAAALELARRYDDLLYLLADVVDSQEGVAPGSSRRVQEHAARFAD
                     ALALTPEEHLALTRAALLRGIGMLNVPVDVRFKKEMLTYDEWALLQAHPKFGADMVSN
                     MSALSDTAPIIRTHHECFDGTGYPDGLEGERIPLLGRMMKILDVYCAMTSPRHYRKGH
                     ATHEQAVQHLKSERGKHFDPKLVDTFIEKEVGQTPES"
     gene            complement(1794..>2182)
                     /locus_tag="PKW60_14345"
     CDS             complement(1794..>2182)
                     /locus_tag="PKW60_14345"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPK00662.1"
                     /translation="GPVIRWVYKGKLFMQVNRKDAKEHIVCYTWIPASALRTLTRLGK
                     RARSNETLEKVTCPVLALHGRHDIAASPRAMEKALDHMASRDKRLVWLERSNHHMYWD
                     YDQEQVFQEVLAFAEKIRGAKNPAPP"
BASE COUNT          399 a          710 c          665 g          408 t
ORIGIN      
        1 tcgtcgcacc gctgctgtcg cgttcgcagg tgttcgaatt gaagccactc gacgacgaac
       61 acgtcgtgga cctgctcaag cgcgtcctga cccatcccga acgcggcatg gcggaacttg
      121 aaatcgaggt ggatgagccc gccctgcgcc atttcgcccg ttatgctgag ggagacgccc
      181 ggcgagccct taacgggctt gaagtggcca tgctgaccac gccgcccgta gacggacgga
      241 tccacatcac cctggatatc gcccaggagt ccattcaacg caaactcatc cattacgatg
      301 gcaccggcga ccagcattac gacgcagcaa gcgccttcat caaaagcatg cgtggcactg
      361 accccgattc cgccctgtat tggatggcgt atatgctcga ggccggggaa gacccgcggt
      421 tcatcgcccg ccgcatctgc atcgccgctg ccgaagacgt gggcaacgcc gacccccagg
      481 cgctggtgct ggccacggct gcatggcagg cctgcgagtt catcggcctg cccgaagcgc
      541 gcatcatcct cgcccaggcc gccacctatg tggcctgcgc gccaaaaagc aatgcggcgt
      601 atctggggat tgacgccgcg ctcggcgacg tccgtgaagg caaaaccgta gcggtgccca
      661 gacatctcca ggacgcgcat taccgcggcg cacaacggct tgggcgcggc gccggctacc
      721 aatacgccca caacgatgaa ggcgggttcg tcgtgcagga atacggcgtg ccgcgatgga
      781 cctactaccg tcctgccggc cgtggcaagg aaggcgaatt caagcagcgc ctcgaagaat
      841 gcgcacgccg cgaggaagca caggaaaacg agcccgggac tccgggcgaa tagtcctgac
      901 ttacctccgg gtcagctctc cggagtctgg ccgacctctt tctcgatgaa cgtgtcgacg
      961 agcttggggt cgaaatgctt cccccgctcg gatttcaggt gttggaccgc ctgctcgtgc
     1021 gtggcgtggc ctttgcggta atggcggggg ctggtcatcg cgcaataaac gtcaaggatc
     1081 ttcatcatgc gtcccagaag cgggatgcgc tcgccttcca ggccatccgg gtagccggtg
     1141 ccgtcaaagc attcatggtg cgtgcgtatg atcggcgcgg tgtcactcag tgcgctcata
     1201 ttcgacacca tgtccgcgcc aaatttcggg tgggcctgca gcagcgccca ctcgtcgtag
     1261 gtaagcatct ctttcttgaa ccgcacgtcc accgggacgt tcagcatgcc aatgccccgc
     1321 aacagcgccg cccgcgtgag cgccagatgc tcttccgggg tcagagcgag cgcatccgcg
     1381 aagcgcgccg catgctcctg gacgcgtctg gagctgcccg gagcgacccc ttcctgtgaa
     1441 tccacgacat cggcgagcag atacagcaag tcgtcgtagc gccgggccag ctcgagcgct
     1501 gccgcgagca cctcgttcgg gtgtctggcg gcgtcaggcg cgatcagacc cgcggacgcg
     1561 ggcgggagcg gggtgccagg cgctacctgg acacactgtg tatccgaatt ctctgccgcg
     1621 gccgatatct ccccaaacca cttctcatcc gtcgtgacca ccgcgatgcg tcgcaacata
     1681 atgctccctt tcctcctggc aatggacccc cgcttttctt cgatccatcc tacgacatga
     1741 ccggtgccga atccaaccgg caaatcgcgt tccgtgccgg gcctgatggt ccatcagggc
     1801 ggcgcgggat tttttgctcc gcgaatcttt tccgcaaagg ccagcacctc ttggaatacc
     1861 tgttcctggt cgtaatccca gtacatgtgg tgattggagc gttccagcca gacgagccgt
     1921 ttgtcccgcg aggccatgtg atccagggcc ttctccatgg ccctgggtga agcagcgatg
     1981 tcgtgccggc catggagtgc cagcacgggg caagtgacct tttccagggt ttcgttcgac
     2041 cgcgcgcgtt tgccgagccg ggtcagggtg cggagggcgg aggcggggat ccacgtataa
     2101 cagacgatgt gttctttggc atccttccga ttgacctgca tgaacagctt gcccttgtat
     2161 acccagcgga tgaccggccc gg
//
LOCUS       DAOPBQ010000579         2181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_39493_length_2181_cov_4.177328, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000579 DAOPBQ010000000
VERSION     DAOPBQ010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39493_length_2181_cov_4.177328"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /gene="proB"
                     /locus_tag="PKW60_14350"
     CDS             <1..579
                     /gene="proB"
                     /locus_tag="PKW60_14350"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPK00663.1"
                     /translation="SDPRRNSEARLIAEVNTITPELEAAAGDTGDQTSVGGMVTKLVA
                     ARIASAAQVRTVIANGRRPNIIHDTLAGQGVGTTFYSSRDSLSQRKRWIAFGRAARGL
                     IRVDDGARNALLKQGRSLLAAGITGVEGRFRMGDAVRVTDAENHDIARGLVNYSSEDI
                     ERIKGCKSTQIDAILGQKHFDEVIHRNNMVVL"
     gene            654..1907
                     /locus_tag="PKW60_14355"
     CDS             654..1907
                     /locus_tag="PKW60_14355"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPK00664.1"
                     /translation="MAIHEEITRICANAREAAQRLRSLSPAVKNAALFNAAQGLRSAE
                     EELKAANARDLEAGRAKGLSSAMLDRLELTSSRIDAMAEGLEAVGALPDPVGDVVRQT
                     VHPNGMRIAQIRQPLGVVAIIYESRPNVTADAAALCLKSGNATILRGGSEAIHSNMAI
                     ARVFMEGAVAAGVPENAVQIITTTDRAAVGALLQMNDYIDVVVPRGGKGLISRILEES
                     RIPVVANLDGVCHVYVDADADPVMARSIVLNAKLQRPGVCNAMETLLVHESIAREFLP
                     ETAQQLEAGGCELRGCARTRQIVECKEATEEDWTTEYLDKVLSIKVVDSLDAAIDHIN
                     RYGSHHSDSIVTAGYEAAERFLDEVDSAAVYVNASTRFTDGFEFGLGAEIGISTNKLH
                     CRGPMALQELTCLKYIIRGAGQIRE"
     gene            1900..>2181
                     /locus_tag="PKW60_14360"
     CDS             1900..>2181
                     /locus_tag="PKW60_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014425452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HPK00665.1"
                     /translation="MSKEQRIGVFGGTFDPIHEAHLRIAKAALQQARLDRVLFVVAAH
                     PPHKSRRRLHASAEERLSMVEAALADEPQMEASRDLWGGCAATTNRTRSR"
BASE COUNT          441 a          667 c          722 g          351 t
ORIGIN      
        1 agcgaccccc gccgcaacag cgaggcgcgc ctgatcgccg aggtcaacac gatcacgccc
       61 gagctggagg cggcggcggg tgacactgga gaccagacct ccgtcggcgg catggtcacc
      121 aagctggtgg cggcgcgcat tgccagcgca gcccaggtgc gcacggtgat cgccaacggc
      181 cggcgcccga acattatcca cgatactctg gccggccagg gcgtgggcac gaccttctat
      241 tcctcgcgcg attctctgtc gcagcggaaa cgctggattg cgtttgggcg cgcggcgcgc
      301 gggctcatcc gcgtcgatga cggcgcccga aacgccctgc tcaaacaggg gaggagcctg
      361 ctggccgccg gcattaccgg cgtggaaggc cggtttcgca tgggcgacgc cgtgcgagtc
      421 acagacgccg aaaaccatga catcgcacgc ggtctcgtca attactccag cgaggatatc
      481 gaacggatca aaggctgcaa gagcacacag atcgacgcga ttctggggca gaagcacttc
      541 gacgaagtca tccaccgcaa caacatggtg gtgctttaag tacgataggt ttaagcacca
      601 gctccaccgg cggcgtgccg cgaagcggca gattctgaag gagagttgag agcatggcca
      661 tacacgaaga aataacgcgc atttgcgcta acgcccgtga agccgcgcag agactgcgtt
      721 cgttgtcgcc ggcagtaaaa aacgcggcgc ttttcaacgc ggcgcagggc ctgcgctcgg
      781 cggaggagga actgaaggcc gccaacgcgc gcgacctgga agccgggcgc gccaagggtt
      841 tgtcgtccgc gatgctggac cgcctggaac tgaccagctc tcgcattgac gcgatggccg
      901 agggcctgga ggcggtgggc gccctgcctg atcccgtggg agatgttgtg cgccagacgg
      961 tgcatcccaa cggcatgcgc atcgcgcaga tccggcagcc gctcggcgtc gtcgcgatca
     1021 tctacgaaag ccgcccgaac gtgaccgccg acgcggccgc gctgtgtctg aaatcgggca
     1081 acgccaccat tctccggggc ggctccgagg ccatacattc caacatggcc attgcccggg
     1141 tgttcatgga aggcgcggtt gcggcgggtg tgccggagaa tgcggtacaa atcatcacga
     1201 ccaccgaccg cgcagccgtg ggcgcgctgc tccagatgaa cgactacatc gacgtcgttg
     1261 tgccccgggg cggcaaagga ctgatttcgc gcatcctcga ggaatcccgt atcccggtgg
     1321 tagctaattt ggatggtgta tgccacgtgt acgtcgatgc ggacgccgac ccggtgatgg
     1381 ccaggtcgat cgtcctgaac gccaaactgc agcgccccgg cgtgtgcaac gccatggaaa
     1441 ccctgctggt acacgagagc attgcccggg aattcctccc ggagacagcc cagcaactcg
     1501 aagccggcgg atgcgaactg cgcggttgcg cgcggacacg ccagatcgtt gagtgcaaag
     1561 aggccacgga agaggactgg accacggagt acctcgacaa ggttctctcc atcaaagtag
     1621 tggactcgct ggatgccgcc atagaccaca tcaaccgcta cggctcgcat cattccgaca
     1681 gcatcgtgac ggcagggtat gaggctgcgg agcgcttcct ggatgaggtg gacagcgcgg
     1741 ccgtgtatgt gaatgcgtcc acgcggttca cggatggatt cgaatttggg ctgggggcgg
     1801 agattgggat cagcacaaac aaactgcatt gccggggccc catggcgctg caggagctga
     1861 cgtgcttgaa atacatcatc cggggagcgg gccagattcg tgagtaaaga gcagcgcatc
     1921 ggcgtcttcg gtggcacctt tgacccgatc cacgaagcgc atctccgcat tgcgaaagcc
     1981 gcgttgcagc aggcgcgtct ggatcgagtc ctgtttgtgg tggcggcgca ccctccccac
     2041 aaatcgcgcc gcaggctcca tgcttcggca gaggagcgcc tgtccatggt cgaggccgcg
     2101 ctggcagacg agccgcagat ggaggccagc cgcgatttgt ggggagggtg cgccgccacc
     2161 acaaacagga ctcgatccag a
//
LOCUS       DAOPBQ010000580         2180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_39522_length_2180_cov_3.800941, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000580 DAOPBQ010000000
VERSION     DAOPBQ010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39522_length_2180_cov_3.800941"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PKW60_14365"
     CDS             complement(<1..483)
                     /locus_tag="PKW60_14365"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPK00666.1"
                     /translation="MGTITDYREARTPLPKTYQAWQIFGAGFENVGKDGQPSTLELRP
                     PKDNEILLRVDALGLCLSDIKIIRQGSAHPRLRGRDLSTDPTVLGHECSVTVVAVGKQ
                     WQDMFKIGERYIVQADIYYDGLSFAFGYMIPGGLAQYSYLDERALAGDEGCYLLPVRD
                     E"
     gene            complement(512..1273)
                     /locus_tag="PKW60_14370"
     CDS             complement(512..1273)
                     /locus_tag="PKW60_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPK00667.1"
                     /translation="MSEILPQLIEMTRYLADPAKGLAILGEGNTSARIDDDTFYVKAS
                     GTSMVNIDAGGFVKVSISKIIPLLDDLEAGDAEVQAAFQDAMIDAPEGKRPSVEAILH
                     AILLQVPEFAFVGHTHPTYTNMILCSRNCAEAARGRLFPDQIVSMKHKSVYVPYTDPG
                     LPLAREVKRRFEAFVNEEGVLPSVILMQNHGLIVMGNTPRAVASATDMTEKASKVLVG
                     TYAMGGPHFLAQKDVERIFTRPDEAYRLKSITGNH"
     gene            complement(1428..1922)
                     /gene="tadA"
                     /locus_tag="PKW60_14375"
     CDS             complement(1428..1922)
                     /gene="tadA"
                     /locus_tag="PKW60_14375"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPK00668.1"
                     /translation="MQNENERYMSYALREAERAMEIGEVPIGCVIVSEGQIVGKAHNQ
                     RELLQDPTAHAEILAITQAATHFQSWRLEGARLYVTLEPCAMCAGAIILARVAEVHFG
                     AYDPKAGVCGSLMNLLEDPRFNHNPRLYPGLLAEECGALLSGFFQRIREDASRHPKPR
                     PPRN"
BASE COUNT          402 a          624 c          709 g          445 t
ORIGIN      
        1 ttcgtcgcgt acgggcagca ggtagcagcc ttcatcgccg gccagggcgc gctcgtccag
       61 atagctgtac tgagccaggc ctccggggat catatagccg aaggcaaagc tcagaccatc
      121 gtagtagatg tcggcctgta caatgtaccg ttcgccgatc ttgaacatgt cctgccactg
      181 ttttccgacc gcgaccacgg ttacggagca ttcgtggccc agtacggtgg ggtcggtaga
      241 caaatcgcgg ccgcgcagcc gcggatgggc gctgccctgc cggatgattt tgatgtcgga
      301 gagacagagg cccagtgcgt ccacgcgaag caggatctcg ttgtctttgg gcgggcgaag
      361 ctcgagggta ctcggctggc cgtccttgcc gacgttctcg aatcccgcac cgaaaatctg
      421 ccaggcctgg tatgtcttcg gcaaaggagt cctggcttcg cgatagtcag taatggtacc
      481 cacaacggca tctccttctc ggtattggtg gctagtgatt tccggtgatg gatttgagac
      541 ggtaggcttc atcgggacgc gtaaagatgc gttcgacgtc tttctgcgcg agaaagtgcg
      601 ggccgcccat ggcataggta cccacaagga ccttcgacgc tttctcggtc atgtcggtgg
      661 cactggctac ggcgcgcggc gtattgccca tgacgatgag cccgtggttc tgcatcagga
      721 tcacggaagg cagcacgcct tcctcattga cgaatgcttc gaaacgccgt ttgacctcac
      781 gagcaagcgg caggcccggg tcggtataag gcacatatac cgacttgtgc ttcatcgaaa
      841 cgatctggtc cgggaagagg cgcccgcgcg cggcttcagc acaattccgc gaacacagga
      901 tcatgttggt gtacgtagga tgggtatgcc cgacgaaggc gaattccggc acctgaagca
      961 agatcgcgtg gagaatggcc tcgaccgacg gccgctttcc ttccggggcg tcaatcatgg
     1021 cgtcttgaaa agccgcctgc acctcggcat cgcccgcctc taaatcgtcg agcaggggaa
     1081 tgattttgga gatggagact ttgacgaagc cgccggcgtc gatattgacc atggacgtgc
     1141 cggaggcctt gacataaaac gtatcgtcat cgatgcgggc ggaggtgttg ccttctccga
     1201 gaatggccag gcctttggcg gggtcggcca gatatcgcgt catctcgatc aactggggca
     1261 gtatttcact catacccggt tcctcttgtt gggagtctgg tggccgcgcg gcgcgaaaat
     1321 ccctgcgcgg cagtcgcgga atgccacaga gtatagcaag tcaggacgca ttatgaaatc
     1381 tgcgcccttc cggggcacgg gagcctggct gcgttctggc gatggcctca gttccgcggg
     1441 ggacggggtt tgggatgccg ggacgcgtct tcacgaatgc gctggaagaa accgctgaga
     1501 agcgcgccgc actcctccgc caggaggccg ggatagagcc tgggattgtg gttgaaccgg
     1561 ggatcttcga ggaggttcat gaggcttccg cagacccctg ccttggggtc gtacgcgccg
     1621 aaatgcacct cggcgacccg ggcaaggatg atggcgccgg cacacatcgc acagggctcc
     1681 agcgtgacgt agagtctggc cccttcgagc cgccagctct gaaagtgcgt ggctgcctgg
     1741 gtgatggcga ggatctcggc gtgcgcggtg ggatcctgca gcagttcgcg ctgattatgg
     1801 gcttttccaa caatttgccc ctcgctgacg atcacgcagc caatgggcac ctcgccgatt
     1861 tccatggcgc gctcggcttc acgcagcgcg tagctcatgt atctttcgtt ctcattctgc
     1921 ataacgcgag gatcttgtcg tttgccccat cgaatgtcaa acagtgctct ggatgttgtg
     1981 ggtttggagg cagcgcccgg tgtcagcgcg gccagacggt ccaggttaac ggaccgggga
     2041 gggtggcgcg gggatggaac cgcatcacgg ccccctgtga ggcggcgtcc agggtcaggc
     2101 cgggatcttc ggcgagaacg acgcggttgc catccatccg ggccgcgcgc agacacaggg
     2161 tccgtccgtc cgcggcttcc
//
LOCUS       DAOPBQ010000581         2178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_39576_length_2178_cov_3.309468, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000581 DAOPBQ010000000
VERSION     DAOPBQ010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39576_length_2178_cov_3.309468"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /gene="priA"
                     /locus_tag="PKW60_14380"
     CDS             <1..1531
                     /gene="priA"
                     /locus_tag="PKW60_14380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPK00669.1"
                     /translation="TEVYLQAIEHALASGRGAIILVPEISLTPQTVGRFKARFQTDIA
                     VLHSGLGAGERYDEWRRAQRGEVRIVVGARSAIFAPLPRLGIIVVDEEHDHSYKQSET
                     PRYHARDVAIMRAKMNDAVCVLGSATPSVESFYNSETGKSIRLELERRATSAALPEVR
                     ILDMRGMLRDQGGQAFLPDALEKAVAERLERSEQVLLLLNRRGFAPFVLCPQCGWMAE
                     CTDCQVTMTYHARGAHLSCHYCNKRRAVPQVCDACHFNPLLYLGVGTQRVEDLLQRTF
                     PQARIERMDADTTAGKGGHAKILGRLAAGEIDVLVGTQMIAKGHDYPGVTLVGVLNAD
                     TGLGMPDFRAAETTFQLLTQVAGRAGRGDKPGHVIIQTYRPNHYAVAAAAGHDYAAFY
                     AREIHERKNAGYPPFRRLANLAIECEEPRGAERAIAALHRLIRREMEALGFRGMEFLG
                     PAPATVSRVKKKYRWNLGVLSVSAKRLNSLVRAARAAFASENPVPSAQLKVDLDPYGM
                     Y"
     gene            complement(1608..>2178)
                     /locus_tag="PKW60_14385"
     CDS             complement(1608..>2178)
                     /locus_tag="PKW60_14385"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00670.1"
                     /translation="VPIRNSRRGHRCIPHSPEAVIARLAEGSLVRAQYMKLDCAVRHG
                     DDEPFCSLSAMNEVNVHMGRINSAVRFTFWLDDKPYDGGTEIIGDGFVISTPFGSTAY
                     FNQITRGIFYQGIGLALKSTTHYTSHVIAPESTVFRVLITRGPAVLAFDSSPEYVHLS
                     RDDELIVRRAPRSATLLLWEPLPYQTDDF"
BASE COUNT          419 a          728 c          658 g          373 t
ORIGIN      
        1 gaccgaggtg tatctccagg ccatcgagca cgccctggcc agcgggcgcg gcgccatcat
       61 cctcgtgccc gagatctcgc tcacgccgca gaccgtcggc cgtttcaagg cacggttcca
      121 gaccgacatc gccgtgctcc acagcggcct gggcgcggga gaacgctacg acgaatggcg
      181 gcgggcccag cgcggtgagg tgcgcattgt ggtgggcgcc cgctcggcca ttttcgcgcc
      241 actgccccgc ctgggcatca tcgttgtcga cgaagaacac gaccattcct ataagcagag
      301 tgaaacgccg cgctaccatg cccgcgacgt cgcgatcatg cgcgcgaaaa tgaacgatgc
      361 ggtgtgcgtg ctcggctccg ccacaccttc cgtcgaatcg ttctacaaca gcgaaaccgg
      421 caaatcgatt cgcctcgaac tcgaacgccg cgctaccagc gccgctctgc ccgaagtgcg
      481 catcctcgac atgcgcggca tgctccgtga tcaaggcggc caggccttcc tgcctgatgc
      541 gcttgaaaaa gccgtggcgg aacgcctcga acggagcgaa caagtcctgt tattgctcaa
      601 ccggcgcggc ttcgccccgt tcgtgctgtg ccctcaatgc ggctggatgg ccgagtgtac
      661 ggattgccag gtcaccatga cctaccacgc caggggcgct cacctgagtt gccactattg
      721 caacaagcgc cgggccgtcc cgcaggtatg cgacgcctgc cattttaacc ctctgctgta
      781 tctgggtgtc ggcacccagc gcgtcgaaga cctcctgcaa cgcactttcc ctcaagcccg
      841 gatcgaacgg atggacgccg acaccaccgc cggcaaaggc ggacacgcca aaatactcgg
      901 ccgcctcgct gcaggcgaaa tcgatgtcct cgtgggcact cagatgatcg ccaaaggcca
      961 cgattatccc ggcgtcactc tggtcggcgt gctcaatgcc gacaccggcc tcggcatgcc
     1021 cgactttcgt gctgccgaaa ccacttttca gctgcttacg caggtcgccg gacgcgccgg
     1081 acgcggcgac aaacccggcc acgtcatcat ccagacttac cggcccaacc attacgccgt
     1141 cgccgccgcg gccggccacg actacgccgc cttctacgcg cgggagattc acgagcggaa
     1201 gaacgccggc tatcccccgt tccgccggct cgccaacctc gccattgaat gcgaagagcc
     1261 tcgcggggcc gaacgcgcca tcgccgcgct ccaccggctc atccgccgcg aaatggaggc
     1321 tctcgggttt cgcggcatgg aatttctcgg acccgcgccc gcaaccgtca gccgcgtcaa
     1381 gaaaaagtac cgatggaacc tcggcgtgct ctccgtaagc gccaagcgtc tcaacagcct
     1441 ggttcgggcc gcccgcgccg ccttcgccag cgaaaatccc gtgcccagcg cacagctgaa
     1501 ggtggattta gacccgtacg ggatgtattg aggcggaggg ggcgagtgct tcgctcattc
     1561 caggggctgt tgccgtgtgt caggcttatt gccggaaatt cagcccgtca gaaatcgtcg
     1621 gtctgataag gaagcggttc ccacaggaga agcgtcgcgg agcgcggagc acggcggacg
     1681 atcagctcgt catcccgact caaatggaca tattccgggg agctgtcaaa cgccagcacg
     1741 gccgggccgc gcgtgatgag cacccggaac accgtgctct cgggagcgat cacgtgagac
     1801 gtgtagtggg tggtagactt cagtgccaga ccgataccct gatagaagat gccccgcgtg
     1861 atctggttga aataggccgt gctgccgaat ggagtgctga tgacaaagcc gtcaccgatg
     1921 atctccgtgc ctccatcata gggcttgtcg tccagccaga aggtgaaccg gacggccgag
     1981 ttgatgcgcc ccatatggac attcacttcg ttcatggcgg acagagagca gaaaggctcg
     2041 tcatcgccgt ggcgcacggc gcagtcgagt ttcatgtatt gggcccggac cagcgagcct
     2101 tcggcgaggc gtgcgattac cgcttcgggg gaatgcggaa tacaccggtg cccgcgccgg
     2161 ctgttccgaa tcggcacc
//
LOCUS       DAOPBQ010000582         2169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_39782_length_2169_cov_3.737938, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000582 DAOPBQ010000000
VERSION     DAOPBQ010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39782_length_2169_cov_3.737938"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKW60_14390"
     CDS             complement(<1..240)
                     /locus_tag="PKW60_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00671.1"
                     /translation="MVSLRTRSAHCALAILIAASLSGWLNAAHALDLVREGAPAAVVV
                     VDESASGQVKAAAQLLAEYVERATGARLPVVNALPP"
     gene            complement(373..>2169)
                     /locus_tag="PKW60_14395"
     CDS             complement(373..>2169)
                     /locus_tag="PKW60_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00672.1"
                     /translation="EWTLLRGLVETPQGNWRVSDAYRPEGRAIKCVRRYVWEGAEAAG
                     RVTLSVRFQTPGTGIGAVLPGILYHGNPSGARSGRTPIYTGAPGEEAFYEEHRFPMPF
                     ASLEWQEGGAFHGAALHALPSPVPFGNLPDQWWSMGLRALDTGTELALLSGPCASNGE
                     RSVVKANQPGFLPYNDASLNVPPGSIIEKTFWLEAYPVPEEGAGFQRPVWTSLSLFDP
                     YCTEGLPTFEEIVRAKYRFSDTRWHETRDAAGYTKYPDKPILVMGWCGQAAALGFANQ
                     VLGPRLGAPEALERAQKSLDFLTNAVFYEGGFHTWYHCNSNTWDHHEPLSQGQAMLSF
                     ANAILAAPKSGLDDSAWRIFLGKACDFHARRILKEDWNPTSTDEGFFIAPLCAAHQIF
                     GQDEYLLAATKAAEVYAARSLSMREPYWGGTLDASCEDKEGAFAAFQGFLALHEVTKE
                     PQYLKWARHACDVALTYVVVWDIDLPPGRLRDHAFKTRGWTAVSVQNMHIDVYGVLIA
                     PYVYKLGLITGSDELKRLALVMYRSCGQLIDPYGSQGEQPQQTNYAQRGKYDTPFGLR
                     GGYVEDWTVFWITAHFLNAAAQFKLMDAETGL"
BASE COUNT          380 a          693 c          688 g          408 t
ORIGIN      
        1 cggcggcagc gcgttgacca cgggcaaccg cgccccggtg gcccgctcca cgtactccgc
       61 caggagctgt gccgccgcct tcacctggcc cgaggcggac tcatccacga cgaccaccgc
      121 cgcgggcgct ccctcgcgga cgaggtccag tgcatgggcg gcgttaagcc atccgctcag
      181 tgaggccgca atcaaaatcg cgagagcgca atgcgcggac cgagttctca aagaaaccat
      241 cgcatttctc cttttttcac tcgcagtcag agctctccgg ggcctccgcc tccacgtgtc
      301 gcgggcaggg tgggatgcgc agcgtgctta tcctcgccgt gcccgggcgt ccgccgcccg
      361 gatttcgcat cattacagcc ccgtttcggc gtccatcaat ttgaactggg ccgctgcatt
      421 caagaaatgc gcggtgatcc agaatacggt ccaatcttcg acgtagccgc cgcgcaggcc
      481 gaagggggtg tcgtatttgc cccgctgagc gtagttggtc tgctggggct gctcgccctg
      541 gctcccgtac gggtcgatca actggccaca actgcggtac atcacgagtg caagccgttt
      601 caactcgtcg ctaccggtaa tgaggcccag tttatacaca tacggtgcga tcaggacgcc
      661 gtacacgtcg atgtgcatgt tctggacgga gacggcggtc cagccgcgcg ttttgaacgc
      721 gtggtcgcgc aggcgccccg gagggaggtc gatgtcccac acgacgacat aggtcagggc
      781 tacatcgcag gcgtgccggg cccatttcag atactggggc tctttcgtga cttcgtgcag
      841 agcgaggaac ccctgaaatg ccgcaaaagc gccttccttg tcttcacagc tcgcgtcgag
      901 ggttccaccc cagtacggtt cgcgcatcga cagcgaccgg gcggcataga cctccgccgc
      961 tttcgttgcg gcgagcaggt attcatcctg gccgaagatt tggtgcgctg cgcacagggg
     1021 ggcgataaaa aagccctcgt ccgtcgatgt ggggttccaa tcctccttca ggatgcgccg
     1081 tgcatggaaa tcgcaggctt tgccgagaaa tatgcgccag gctgagtcgt ccaacccgga
     1141 tttcggggcc gccaggatgg cgtttgcgaa actgagcatc gcctggccct gcgacagcgg
     1201 ttcgtggtgg tcccaggtat tgctgttgca gtgataccaa gtgtgaaatc cgccctcgta
     1261 aaaaacggcg ttcgtcagga aatcgagtga tttctgcgcc cgttccaggg cttccggtgc
     1321 gcccaggcgg ggccccagca cctggttggc gaatccgagc gcggccgcct gcccgcacca
     1381 gcccatgacg agaatgggtt tatcggggta tttcgtgtaa ccggccgcat cccgggtttc
     1441 gtgccagcgg gtgtcggaga agcggtattt tgcgcgaaca atctcttcga aagtcggtaa
     1501 gccttcggtg caatagggat cgaacaggct cagactggtc cagaccggcc gctgaaatcc
     1561 cgcgccctcc tcgggcaccg ggtacgcttc gagccagaag gtcttttcaa tgatcgagcc
     1621 cggcggcacg ttcaaactgg cgtcgttgta cggtagaaag cccggttgat tggccttgac
     1681 cacgctgcgc tccccattgg acgcgcaggg gcctgacaac agggccagtt ccgtgccggt
     1741 gtcgagcgct cgcagcccca tcgaccacca ttgatcgggc aggttcccga atggcacggg
     1801 ggagggcagc gcgtgcaggg cagcgccatg gaaagcgccg ccttcctgcc attccaggct
     1861 tgcgaagggc atggggaagc ggtgttcttc gtagaacgct tcctcgcccg gcgcccccgt
     1921 gtagattggc gtccggccgc tgcgcgcgcc cgacgggttg ccatggtaca ggatgcccgg
     1981 gagaacggcc ccaatccccg tgccgggtgt ctggaagcgc acggacaagg tgacgcggcc
     2041 ggccgcttcg gcgccttccc agacataccg gcggacgcat ttgatcgccc ggccttccgg
     2101 ccggtaggcg tcggatacac gccagttacc ctgaggcgtc tccaccaggc cgcgcagcag
     2161 agtccattc
//
LOCUS       DAOPBQ010000583         2163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_39909_length_2163_cov_4.251423, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000583 DAOPBQ010000000
VERSION     DAOPBQ010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39909_length_2163_cov_4.251423"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..495)
                     /locus_tag="PKW60_14400"
     CDS             complement(40..495)
                     /locus_tag="PKW60_14400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPK00673.1"
                     /translation="MAINPKYEMLAKRVALFKGINPEDVSKIVARGLVMTMEKGNTIF
                     YKGTTGNQMFVILAGRVSLFDGQKHLADLRTGDMFGEMALINNEPRSATAVVAETAQL
                     FVLSETFFQKLMTKRIAITMLLNIIGTLSTRLQGMNRRLLELKQESPSS"
     gene            complement(597..1427)
                     /locus_tag="PKW60_14405"
     CDS             complement(597..1427)
                     /locus_tag="PKW60_14405"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPK00674.1"
                     /translation="MNLTKYILKSCIVPEMESDCKADALKELLNLLFEKKKMTSPGPA
                     LDQILAREVTESTGIGKGIAVPHARVTGMKGLACAVGRVPEGMDFHAIDRKPVHLIFL
                     ICYPPNEQTTYLNFVATVAKLLSDPQNLAQIMNADNEDQIFEVLEASSVQFSESSEQP
                     LRKVKTDPAIEQVQDGHAEVILLARMQLYEEMLSSARSGKKQIRERIDHIRELVSPRV
                     IRHYDRVNKVRPPALVPVEGDTCQGCFMKLPSQFVQQVRQDPEHIHTCPNCSRYIYVV
                     "
     gene            complement(1859..>2163)
                     /locus_tag="PKW60_14410"
     CDS             complement(1859..>2163)
                     /locus_tag="PKW60_14410"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPK00675.1"
                     /translation="DTVIDAGGGVVQRPENVALLKTNGAFFWLTAEVDTIKERIQNDG
                     GRPSITGRKSFIEEVEEVLEARLPFYEAAADYVIRTDNRTLGEIADEIVEIACAAL"
BASE COUNT          403 a          633 c          631 g          496 t
ORIGIN      
        1 agcagtctag cgggcggcag agatcgcatc aagtccggtt cacgaagacg ggctctcctg
       61 tttcaattcc aggagccgcc ggttcatgcc ctgcagacgc gtgctgagcg tgccgataat
      121 gttcaggagc atggtgatcg cgatccgctt ggtcatcagt ttctgaaaaa aagtctcact
      181 gagaacgaac aattgcgcgg tttcggcgac taccgccgtg gcgctgcgcg gttcgttgtt
      241 aatcagggcc atttcgccaa acatgtcgcc agtgcgcaga tcggccaaat gtttctggcc
      301 atcaaaaagg ctgaccctgc cggccagaat aacgaacatc tgattgcccg tcgtgccctt
      361 gtagaagatg gtattgcctt tttccatggt catgacgaga ccgcgggcga caatcttgct
      421 tacgtcttcg ggattgatgc ctttaaacaa agccacccgc tttgccagca tctcatattt
      481 ggggtttatg gccatgggag gcgtcctttc tgcaggcgct ctgcgcacgc caaccccgca
      541 tcccccgccg gcatttccgg cggaggctgg gtgcatttcc gcatgccggc ctgcttttat
      601 accacgtaaa tgtagcggct gcagttgggg cacgtatgga tgtgttcggg atcctggcga
      661 acctgctgca caaactggga cggcagcttc atgaaacagc cctggcaggt atcgccttcg
      721 accgggacca gcgcgggggg ccgcaccttg ttcacgcggt cgtaatggcg gatgacccgc
      781 ggggacacca gctcgcgaat gtgatcgatg cgctcccgga tctgcttttt ccccgaacgg
      841 gcgctgctga gcatttcctc atacagctgc atacgggcaa ggaggatgac ctcggcgtgc
      901 ccgtcctgta cctgctcaat ggccgggtcg gtttttactt tgcgcagcgg ctgttccgaa
      961 gattccgaga actgcacgga cgacgcctcg agcacctcga aaatctgatc ttcgttatcg
     1021 gcgttcatga tctgcgccag gttctgcggg tcgctgagga gtttggctac cgtggcgaca
     1081 aagttcaggt acgtggtctg ttcattgggg ggatagcaga tgagaaagat gagatgcacg
     1141 ggtttacggt cgatggcatg gaaatccatg ccttccggga cccggccgac agcgcaggcg
     1201 agccccttca tgccggtcac ccgcgcatgg ggcacggcga tgcctttgcc gatgcccgtg
     1261 ctttcggtca cttcgcgcgc gaggatctgg tcgagcgcag gaccggggct ggtcatcttc
     1321 tttttttcaa aaagcaggtt cagcagctct ttgagagcgt ctgccttaca gtcggactcc
     1381 atttcgggca cgatacatga tttgagaatg tacttggtca gattcatcga tacacctgtc
     1441 cttcctgtgt gcgcgcgcgt tgatggcccg gaccggctgc tacgacgtgt ttgattggtc
     1501 gaggaatgcc ccatgcgcaa gcctcctcgc tggcagccgc atgcccccgg ctgctgacct
     1561 caatatcaga ccgtagtgct aagcgtaggc tcctcggctt tagcgccatg atgatgctcg
     1621 taactgaacg tgtatatatg ggacgagctt cgcctcaacc caattcgttt ccaggcaacg
     1681 gccccggccg tacccgcccg gcggggaggt tcggcgctca cccttgtctg gagcagatga
     1741 attcgcctgt ccggcccccg gctggacgct ctcgccaggc ttccggagct tgtttccagg
     1801 cggttcattc cctccctgaa cgtaatgcag tatagcacgc tgacgcggga taaaatagtt
     1861 acaatgccgc acaggcgatc tcgacgatct cgtccgcaat ctcccccagg gtgcgattgt
     1921 cggtacgaat gacatagtcc gcggcggctt cataaaacgg gagccgggcc tccaggactt
     1981 cctcgacttc ttcgatgaac gatttccggc cggtgatgga agggcgcccc ccgtcgttct
     2041 gaattcgttc cttgatggta tccacctccg cagtcagcca gaagaaggcg ccgttggtct
     2101 tgagcagtgc cacattctcg gggcgttgca ctactccgcc acccgcatcg atgacggtgt
     2161 ctt
//
LOCUS       DAOPBQ010000584         2151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_40208_length_2151_cov_3.204676, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000584 DAOPBQ010000000
VERSION     DAOPBQ010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40208_length_2151_cov_3.204676"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..1527
                     /locus_tag="PKW60_14415"
     CDS             397..1527
                     /locus_tag="PKW60_14415"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK00676.1"
                     /translation="MRWRLAHVGLVGMALLCAGCATTGHSPKQDLRAASAPVWSLQQG
                     NDELAVFMSPTGRNLRLAGSVGVVLGTGVDAVVNARYRSRIQELIGQDDTAVLLADIL
                     EKALAEAAGPSLEQAAPLPSTAGFDSRRDAFSAYYDGLARAGHDVLLRLRSTHGIFGH
                     DATLAVKLDGRLVELPSGHTLWANEIVTTVEPVMAYDRLGLPTDRMAPRIKGARLTVE
                     EDALAKWESGGRSLRADFEEAAKAAASALVVNLGLAEDALGEYHLGELALMRKDFNEA
                     LRHYRKAISLNPDFPAAHNGIAVTLGHGGEIADAIETATAVTEQWPEFAPSYMNLAWW
                     YATEKKDIESAQPYYRRALELGMAPVPRMNELLYGKGKKSDQ"
     gene            complement(1546..>2151)
                     /locus_tag="PKW60_14420"
     CDS             complement(1546..>2151)
                     /locus_tag="PKW60_14420"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPK00677.1"
                     /translation="RNFVLTKEDIQGAFAAVRPLLRLKHVLPSGAGIFEDLEAKPRAW
                     LTFEWRPVESFDPDLLDSALPPLIEGPVPPAVEGAQKATATILPESGNACVRIAVESE
                     KPGVLCLADTWFPGWSAQINGKPTAIFPLDGLFRGIHVPEGASTIEMVYEPRSLVVGA
                     WISLGGALFTSGFILYLAFRWLMKRHRARRLERELQASPMP"
BASE COUNT          414 a          669 c          675 g          393 t
ORIGIN      
        1 gatgcatccc cgccactacc gttggggagg tggcacggct tcctgtctcg gttcgcatgc
       61 acatgacgcc ccacgatagc tcccggacac tcgtgtttca ccgggcatag tccagtttct
      121 tctagactgc ccctttccag gcacaagatg caaattaccg gggccggccg aattgccagt
      181 ccgcgagatc cggcggaaac gcggcatgcc gggcgaatga attgaatgcc gtgtaagacc
      241 ggcaaatccc gccccagtcg agacaagttt gcgctggcgc ctgcgcacag gtagcataca
      301 tccccgcatc tccagatact gaaatctgca tgcatgcatt ctcagtcagt gcataccacg
      361 gttttcccgg cgggaaattc aagaggagtc ttcataatgc gttggcgttt agcgcacgtt
      421 ggcctcgtgg gcatggcctt actctgcgca ggctgtgcca ccacagggca ttccccgaag
      481 caagacctgc gcgcagccag tgccccggtc tggtcgttgc agcagggcaa cgacgagctc
      541 gcggtcttca tgtcgcccac ggggagaaac ctgcgcctgg cggggtcagt cggggttgtc
      601 ctggggacgg gtgtcgacgc cgtcgtgaac gcccggtacc gcagccggat tcaggaactg
      661 atcggtcagg acgataccgc ggttctgctg gccgacatcc tggagaaggc cctcgccgaa
      721 gccgcgggcc cctccctcga acaagccgct cctctgccga gtacggccgg ttttgacagc
      781 aggcgggatg ccttctccgc ctattatgac ggccttgcac gggccggcca cgacgtgctg
      841 ctgcggcttc ggtcgacgca cgggattttt gggcacgatg ccacactggc ggtcaaactg
      901 gacgggcgcc tggtcgaact gccgtccggg catacgctgt gggccaacga gatcgtgacc
      961 accgtggaac ccgttatggc gtacgaccgg ctgggccttc cgacggatcg catggcgccc
     1021 cgcatcaaag gggcgcgcct gaccgtcgag gaggatgctc tcgcgaagtg ggaaagcggc
     1081 gggcgatcct tgcgggcgga tttcgaggag gcggcgaagg ccgccgcgtc ggccctggtc
     1141 gtgaatctgg ggctcgcgga agacgccctc ggcgaatatc atctcggcga attggccctg
     1201 atgcggaaag atttcaatga agccctgcgg cattaccgca aagccatttc actgaacccg
     1261 gactttcctg ccgcgcacaa cggcatcgcc gtgactctgg gccacggggg agagatcgcg
     1321 gatgctatcg aaactgcaac ggcagtcacc gagcaatggc cggaattcgc cccgtcctat
     1381 atgaatctcg cctggtggta tgcgacagag aaaaaggaca tcgaatcggc gcagccatac
     1441 tatcggcgcg ccctcgagct cggcatggcg cccgtcccgc gaatgaacga gttgctctat
     1501 ggcaagggga aaaagagcga tcagtaatcc cggttccccg gttggctacg gcatcggaga
     1561 tgcctgcagc tcccgttcga gtcggcgggc gcggtgccgc ttcatcagcc accggaacgc
     1621 cagatagaga ataaatcccg aggtgaagag cgccccgccg agactgatcc aggcgccgac
     1681 gaccaggctg cgcggttcgt agaccatctc gattgtgctg gcgccttccg gtacgtgtat
     1741 tccccggaac aggccatcca gcgggaagat cgccgttggt tttccgttga tctgggcgct
     1801 ccagcccgga aaccacgtgt cggcgagaca caggactccc ggtttttcgg actcgaccgc
     1861 gattcgcacg caggcattac cgctttcagg aaggatagtc gccgttgcct tttgagcacc
     1921 ctctacggcg ggagggaccg gcccttcaat caagggcggc agcgcgctgt ccagcaggtc
     1981 aggatcaaac gactccacgg gccgccattc aaatgtgagc caggcgcgcg gcttggcctc
     2041 cagatcctcg aaaattcccg ctccagacgg cagcacatgt ttcaggcgca gcagaggccg
     2101 gacggcggca aaggcccctt gaatgtcttc tttggtgagc acgaagttgc g
//
LOCUS       DAOPBQ010000585         2137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_40594_length_2137_cov_2.987512, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000585 DAOPBQ010000000
VERSION     DAOPBQ010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40594_length_2137_cov_2.987512"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PKW60_14425"
     CDS             <1..331
                     /locus_tag="PKW60_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00678.1"
                     /translation="GSIGNFHRYAQDILREFQYRDFYTHWSDVVTRALAEFPVDDLWH
                     LNAEKRASQPTSQTAWDLRRALSRIDTLYGQGKLREAAQEIESNAPTFRMFAPEPVSE
                     QAAPSGE"
     gene            551..>2137
                     /locus_tag="PKW60_14430"
     CDS             551..>2137
                     /locus_tag="PKW60_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00679.1"
                     /translation="MPKSPILRLGSLLCLFFFATAPVRADEWTLVLPPSLAADTAMAL
                     ALEDLVNTGAEYGHSFSIADDSALPSGGVILVGGSERNVQTKALMDLGILTARAPENE
                     QGYALQTVTQDGRKLVSVAGGSVIGDVYGVYWLWDRLRIHRALPDVNTTRVPAMALRM
                     GAAWGRSGPGGNSKQELQNALRDSINWVAGPNVLDLVPWDAEPENTANAAHRERVREL
                     IDYAHALHMKYFSFSNEFTYHPSLLAEHGATLDPCDPKLWDAIQDKYRKLFTALPELD
                     GIELCNDDISGFWDDYAAYDVLHENPDCPTCYTQRFTQFVKKVHEVVAGEFNKTYFHF
                     TWGLVMHEQHYQPAVYREIFTDAVPRDNLYLVPKITAGDRWWYQPYNETFNQTPHNTL
                     VCFETMNVYEGGRSHIFPTFAGQYYQNGLQYLLLPETNNLRGAALLAARSARGWDTLG
                     AYAYVLYRLMWDPYDDIEAIARDFCSICFGPEAADRMAEVYLLSPSAYQYGLHIGSIS
                     YGQFNSFLHMRVNEFPVEGYP"
BASE COUNT          443 a          663 c          587 g          444 t
ORIGIN      
        1 cggcagcatc ggcaactttc accgctatgc tcaggacatt cttcgcgaat ttcagtatcg
       61 tgacttttat acccattggt cggatgtcgt gacgcgcgca ctggccgaat ttcccgtgga
      121 tgacctgtgg catctcaatg ccgaaaagcg cgcgtctcaa ccaacatccc agacggcctg
      181 ggacctccgc cgcgccctct cccggatcga cacgctctac ggccagggga aactgcgcga
      241 agccgcacag gaaatagagt cgaatgctcc cacgtttcgc atgtttgctc cggaacctgt
      301 ctcggagcag gcagccccgt ccggcgaata gccgatggca gataagctca gccgccagtg
      361 aggcggactt gcggcagcgg cgaatccttg catgacattc tctcttttgg catactgcgt
      421 tcttccagtg ggcaagacaa ctccactcaa ttctgccgtc catgggggcg tcaatcaggg
      481 attttttacg ggtactgcct gaaacatccc tcactgtcga acatctcaac atcaaagaag
      541 ggtcaaattc gtgccaaaat caccaattct ccgactggga tctctcctgt gcctgttttt
      601 tttcgcaacg gcgccggtgc gcgccgatga atggactctg gttctcccgc cgtccctggc
      661 agcggacacc gccatggcgt tggcgctcga agacctggtg aacacggggg cggagtatgg
      721 acactcgttt tctatcgcag acgacagcgc tctgccgtcc ggaggcgtca tcctcgtggg
      781 cggcagcgag cgtaacgtgc aaacgaaggc cttgatggat ctgggaatcc tgacggcacg
      841 cgcccctgaa aatgaacagg gctacgcgct tcaaaccgtg acacaggatg gccgaaagct
      901 tgtctcggtt gccggcggct cggtcattgg cgatgtctat ggcgtgtact ggctgtggga
      961 tcggcttcgc atacaccgcg ccctgcccga tgtgaacacc acccgggtac ccgcgatggc
     1021 actccgcatg ggcgctgcct gggggcgttc cggccccgga ggcaacagca agcaggagct
     1081 ccagaatgct ttgcgcgact cgatcaactg ggtcgccggg cccaatgtgc tcgatctcgt
     1141 cccatgggat gctgaacccg aaaatacggc gaacgcggcc caccgtgaac gggtgcggga
     1201 attgatcgac tacgcccatg cgctgcacat gaaatacttc agtttctcaa acgagttcac
     1261 ctaccatccg tctctgctcg cggagcacgg cgcgaccctt gatccctgcg atccaaaatt
     1321 gtgggacgcg atacaggaca aatatcggaa gctgttcacg gccctgccgg aactggacgg
     1381 catcgagctc tgtaatgacg acatctccgg gttctgggac gactacgccg catacgatgt
     1441 gctgcatgaa aatcccgatt gcccgacgtg ctacacccag cgattcactc agtttgtcaa
     1501 gaaagtgcac gaagtggtgg cgggagagtt taataagacc tatttccatt tcacgtgggg
     1561 cctcgtgatg cacgagcagc actaccagcc cgctgtttac agggaaattt ttaccgatgc
     1621 cgtgccgcgc gacaacctct acctcgtccc caagatcacc gcgggcgacc ggtggtggta
     1681 ccagccctac aacgagacct tcaaccagac ccctcacaac acgctcgttt gctttgagac
     1741 catgaatgtg tacgaaggcg gcaggagcca catcttcccc accttcgcgg ggcagtacta
     1801 ccaaaacggg cttcaatacc ttcttctgcc ggaaacgaac aacctgcgcg gcgcagcatt
     1861 gctggcggcc cgctcggccc ggggatggga caccctcggc gcctacgcct atgtgctcta
     1921 ccgcctgatg tgggacccct acgacgacat tgaggccatt gcgcgcgatt tctgctccat
     1981 ctgtttcgga cccgaagccg cggaccgcat ggcagaggtc tatctgcttt cgcccagtgc
     2041 ctatcaatac gggctgcaca tcggctcaat ttcctacgga cagttcaatt cgttcctgca
     2101 catgcgtgtc aatgaattcc ctgtcgaagg ctatccg
//
LOCUS       DAOPBQ010000586         2135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_40643_length_2135_cov_4.657692, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000586 DAOPBQ010000000
VERSION     DAOPBQ010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40643_length_2135_cov_4.657692"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PKW60_14435"
     CDS             <1..257
                     /locus_tag="PKW60_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HPK00680.1"
                     /translation="QEKPQAAGGYINGGFFVCEPGVFDYVSEDEACTFEREPLQRLAR
                     DGQMMTYYHDGFWMPMDTFREYTLLNEMWSRGDAPWLKKA"
     gene            302..1435
                     /gene="rfbG"
                     /locus_tag="PKW60_14440"
     CDS             302..1435
                     /gene="rfbG"
                     /locus_tag="PKW60_14440"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HPK00681.1"
                     /translation="MPFGNFYQGKKVLVTGHTGFKGSWLASWLELLGAEVYGIALEPS
                     TTPALFTLLRLEDRLDHTILDIREPEPLAERIAAIRPDLVFHLAAQPLVRLSYEIPLE
                     TLATNVMGTAHVLCAIDRAGYGPERPCGVVVITSDKCYENRETYTAYREDEPMGGHDV
                     YSASKGMAELLVASWRRSFHNGADGVPAIRIASCRAGNVIGGGDWSADRIMVDAVNAL
                     MKEQAIPVRNPLSVRPWQHVLEPLSGYLQIGAALMTGDKAQQYCTSWNFGPGRESERT
                     VAELCDALVAAWGGGAWAHSPDHEAVHEARFLKLAIDKAWHFLGWQPVWDFRATVEQT
                     VSWYKLAHESGFDPAKLLNKTHDQIRGFVQDAAARGLPWADSK"
     gene            1448..>2135
                     /locus_tag="PKW60_14445"
     CDS             1448..>2135
                     /locus_tag="PKW60_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPK00682.1"
                     /translation="MSSTALFSLTGRRVLITGIQGFIGIHTAAAAQRLGAQAAGIDLA
                     QESPRARQVRESLGMRPVEIHAADLMEQQAIVDILKEVRPAVIVHCAGSIERSTGASD
                     WARVFAGNADVTASLVGALLSLPAAERPVLVMPGSQMEYGTAPMPWTEDRHCEPVNAY
                     GAGKLAATEHVLAAARGHGLRACVLRLPIVFGPGQPPALFIPELIGKALRREAILMTE
                     GNQRRAFLYVE"
BASE COUNT          405 a          672 c          685 g          373 t
ORIGIN      
        1 ttcaagagaa gccgcaagcc gcgggaggct atatcaacgg cggatttttt gtctgtgagc
       61 cgggggtttt tgactacgta agcgaggatg aggcctgcac cttcgagcgg gaaccgctgc
      121 aacgcctcgc gcgcgacggc cagatgatga cctattacca tgacgggttc tggatgccca
      181 tggatacgtt ccgcgaatat acgctgctga acgagatgtg gagccgcggc gacgcaccgt
      241 ggctgaagaa agcctgaccg cgccgctgga gcgagcccat tgcaggtaag gagagccacc
      301 catgcctttc ggcaatttct atcagggcaa aaaagtgctg gtcaccgggc ataccgggtt
      361 caaaggctcc tggctggcct cctggctcga actcctgggc gcggaggtct acggcatcgc
      421 cctggaaccc agcacaaccc ccgcgctgtt cactctgctg cgccttgaag accgcttgga
      481 tcacaccatt ctggacattc gcgaaccgga gccgctggcg gaacggatag cagccatccg
      541 cccggacctg gtgtttcatc tggccgccca gccactggtg cgcctgtcct atgaaatccc
      601 gctcgagacc ctggcaacta atgtcatggg cacggctcat gtactctgcg cgatagaccg
      661 ggcgggatat gggcccgaac gcccgtgcgg cgtcgtcgtc atcacctcgg acaagtgcta
      721 cgagaaccgg gaaacgtaca cggcctaccg cgaagacgaa cccatgggcg gccacgacgt
      781 gtacagcgcc agcaaaggca tggccgagct gcttgtagcc tcgtggcgcc ggtcattcca
      841 caacggggcg gatggcgttc cggccatccg gatcgcgagc tgccgcgccg ggaatgtgat
      901 cggcggcggc gactggtcgg ccgaccgcat catggtcgat gccgtgaacg ccttgatgaa
      961 agaacaggca atccccgtgc gaaacccgct ctccgtccgc ccgtggcagc acgtgctcga
     1021 gccattatcc ggctacctgc agatcggcgc ggcactcatg accggcgaca aggcgcagca
     1081 gtactgcact tcgtggaatt tcggccccgg ccgcgaatcg gaacgcacgg tagcggagct
     1141 ttgcgacgcg ctcgtggccg cctggggcgg cggcgcctgg gcccactccc ccgaccacga
     1201 ggcggtgcat gaagcacgct ttctgaaact cgccatcgac aaagcctggc atttcctcgg
     1261 ctggcagccg gtctgggatt tcagggcgac cgtggagcag acggtatcct ggtacaagct
     1321 ggcgcatgaa tccgggtttg atccggcgaa attgctcaac aagactcacg atcagatccg
     1381 gggctttgtg caggacgccg cggccagggg tttgccctgg gctgattcca aataggccgg
     1441 aggcggcatg tcgagcacag cgcttttttc tctgaccggc cgcagggtcc tgattacagg
     1501 cattcagggc ttcattggca ttcacacagc cgcggcggca cagcgcctcg gcgcacaggc
     1561 cgcgggaata gacctggctc aggaatcccc gcgggcgcgg caggtccggg aatcactggg
     1621 catgcggccc gttgaaatcc acgccgcaga cctcatggaa cagcaggcca ttgtcgacat
     1681 cctgaaagaa gtgcggcccg ctgtgatcgt gcactgcgcg ggctcgatcg agcgcagcac
     1741 gggtgcgtca gattgggccc gggttttcgc gggaaacgcc gatgtgacgg cctcactggt
     1801 cggcgccctg ctctctctgc cggctgctga gcgcccggtc ctggtgatgc ccggctcaca
     1861 gatggaatac gggacggcgc ccatgccgtg gaccgaagat cgccattgcg agccagtcaa
     1921 cgcctacgga gcagggaaac tcgccgccac ggaacatgtg ctcgcggcag cgcgagggca
     1981 tgggctgcgc gcatgtgtcc tgcggctgcc catcgtgttt ggaccgggcc agcctccggc
     2041 gctgttcatt cccgaactta taggaaaagc cctccgcagg gaggccatcc tcatgaccga
     2101 aggcaatcaa cgccgggcct ttctgtacgt tgaag
//
LOCUS       DAOPBQ010000587         2132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_40717_length_2132_cov_3.503130, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000587 DAOPBQ010000000
VERSION     DAOPBQ010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40717_length_2132_cov_3.503130"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="PKW60_14450"
     CDS             <1..1380
                     /locus_tag="PKW60_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00683.1"
                     /translation="VIDDFLCTGDVSEESKEAKGEAPWSQYRCELMAAVARDVFITPA
                     REANPDITMIVKFPQWYDLFHEYGYALASHAHSFDRVWVGTETRGARTQRYGFVQPFE
                     AFVNYRWIASVAPKTIQAAWFDHGDCDALDFVDQAYQSVLAGARILSVFNFLDVLNGH
                     PGDELLAQEFDRLADLAAAVKTGPVQGVAAYKPVGSDAGGNLYVMDFIGMLGVPLVPM
                     VAFPHEAKVLFLPEQAASDPGVIGKLRARLSRGGRVIVTAGFLARRPEAAELAGLEPV
                     HLDPVRAEAVLLNGEPVPVQHGLDLAALLKPGDADVLLEALVDSTPVPFLIRRRFQNS
                     GLYVLNCHTFSQADFDAVGEVLLAPRELGLLELPQGATDLLRAVFLEPLGLALDAPTR
                     VCLQPFGEQEFFVQNYNETPVSAVLRAPASVAGGTWHNQLTGAEIPVSDGNLIIDLGA
                     RSRCWLKPQ"
     gene            complement(1405..>2132)
                     /locus_tag="PKW60_14455"
     CDS             complement(1405..>2132)
                     /locus_tag="PKW60_14455"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPK00684.1"
                     /translation="MLFHALGAYGLLEKTLVVIASDHGEEFLDHDWVEHGWSLHEEVL
                     RVPLIFWAPGKVPAARVQAPVSVVDLYPTMAQLFGLPTDGSTLDGEALFKPANPGLRL
                     TPPSHPYIAELMLQERAMTRCVIVDGWKYLAARKRFTPSERPREVRQLEETQLGLQAG
                     TIPMTDVWGPPVFEELYCLSKDPRETTNVLDEETAVRDKLRAILEEHEKKCRERGIET
                     PPRQQVPLLSPEEMDLLHSMGYF"
BASE COUNT          393 a          639 c          646 g          454 t
ORIGIN      
        1 gtcattgacg attttctctg cacgggagac gtcagcgaag aatcaaagga ggcgaaaggc
       61 gaggccccct ggagccagta tcgctgcgag ctgatggccg cggtggcccg ggatgtgttt
      121 attactcccg ccagggaagc gaatccggac atcacgatga tcgtcaagtt tccgcagtgg
      181 tatgacctgt ttcacgaata tgggtatgcg cttgccagcc atgcacattc ttttgaccgc
      241 gtctgggtgg gcacagaaac ccgcggcgcc cgaacgcaac ggtatggatt tgtgcagcct
      301 ttcgaagcgt tcgtgaatta ccggtggatt gcctcagtcg cgccgaagac catccaggcc
      361 gcgtggtttg atcatggcga ctgtgacgcc ctcgattttg tggatcaggc ataccagtct
      421 gtcctggcgg gcgcacgcat tctcagtgtc ttcaattttc tggatgtctt gaacgggcac
      481 ccgggagatg aattgctggc acaggaattt gaccgtctgg ccgatctcgc ggcggcggta
      541 aagacgggtc ccgttcaagg agtcgcagcg tacaaacccg tgggcagcga tgcaggcgga
      601 aacctgtatg tgatggattt tattgggatg ctgggggtgc cgcttgtccc aatggtggcc
      661 tttcctcacg aagcgaaagt gctcttcctt cccgaacagg cggcgtcaga tcccggtgtc
      721 atcgggaagc tgcgcgcacg gctctctcgc ggcgggcgcg tcatcgttac cgcgggcttt
      781 cttgcgcgcc gcccggaagc cgccgagctg gctgggctcg aacccgttca cctcgacccc
      841 gtccgggctg aggcggtcct cctgaatgga gagcccgtgc ccgtgcaaca cggcctggat
      901 ctggccgcgc ttctgaaacc aggcgatgcg gatgtcctct tggaggccct ggtggattcg
      961 acgccggtcc ccttcctgat ccgacgccgt ttccagaaca gcggactcta tgtgctcaac
     1021 tgccacacgt tctcgcaggc cgattttgat gcggtggggg aggtgctcct ggcgccgcgt
     1081 gaactggggc tccttgagtt gccgcagggg gcaactgacc ttcttcgcgc ggtttttctg
     1141 gagcctctcg gcctcgctct ggacgcgccc acacgcgtgt gcctgcaacc gtttggagag
     1201 caggagtttt ttgtccagaa ctacaatgag acgccggtaa gcgccgtctt gcgggcgccg
     1261 gcatcagtcg ctggcggcac gtggcacaac cagcttaccg gcgctgaaat tcctgtatcc
     1321 gacggaaatc tcattatcga cctgggcgcg cggagccgtt gctggctcaa gcctcaataa
     1381 gcgcaaattc gaggcagatg ccgctcagaa ataccccatg ctatggagaa gatccatttc
     1441 ttccgggctc aacaggggaa cctgctgccg gggcggcgtc tcgatacctc gctcgcggca
     1501 cttcttttcg tgttcctcga gaatagcgcg cagtttatcg cggaccgcag tttcttcgtc
     1561 cagcacgttc gtggtttccc ggggatcttt ggaaagacag tagagttctt cgaaaaccgg
     1621 cgggccccag acatctgtca tcgggatggt gccggcctgt aaccccagtt gcgtttcctc
     1681 aagctgcctg acctctctgg gccgctcgct cggagtgaaa cgcttgcgcg cagccaggta
     1741 cttccaccca tccacgatca cacagcgtgt catggcgcgc tcctggagca ttaattcggc
     1801 aatatagggg tgggagggag gcgtcaaccg caggcccggg ttcgcaggct tgaaaagcgc
     1861 ttcgccgtcc aatgtagagc cgtccgtcgg caatccgaac agttgtgcca tcgtagggta
     1921 caaatccacc accgacacgg gggcctgcac gcgtgccgcc gggacttttc ccggggccca
     1981 gaaaatcaga gggacgcgaa gcacctcttc gtgcagtgac catccatgct ctacccagtc
     2041 gtggtccaga aattcctccc catgatccga tgcgatcacc accagggtct tctcgagcag
     2101 gccataggcc cccaacgcgt gaaacagcat ct
//
LOCUS       DAOPBQ010000588         2111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_41260_length_2111_cov_3.547179, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000588 DAOPBQ010000000
VERSION     DAOPBQ010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41260_length_2111_cov_3.547179"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PKW60_14460"
     CDS             complement(<1..925)
                     /locus_tag="PKW60_14460"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPK00685.1"
                     /translation="MKVGIDIGSVTGNRTGIGNYCYYLVKHLLQLDDTWELKAFSTGL
                     RRIDARQWGRPLFHRHLPIPTRLAYTMWEHWGKPAVDTLLHGVDVYHATNYFLPPTSV
                     AKRVVTIHDLSFLAAPHLCSPRIVGPFSRHIARFAREAHGILVYSESTARDIERLLEV
                     PSTKIHVAPLAVDESIGPMPREEAAAQVCRICGVQGPFILFVGMIEPRKNIPTLLRAF
                     QKIAREIPHQLVLAGPLGWNPEEYQHVRDEMQLGDRVVHTGYVDDHAALAAFYSAADL
                     FVFPSLYEGFGLPVLEAMACATPVAAARPADA"
     gene            1139..>2111
                     /gene="hrcA"
                     /locus_tag="PKW60_14465"
     CDS             1139..>2111
                     /gene="hrcA"
                     /locus_tag="PKW60_14465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPK00686.1"
                     /translation="MRGHTLLEQGAEQLSQRERLILQAVVHSYITTAEPVGSRAVVKR
                     FGLDISPATVRNVMADLEEAGYLKQLHTSSGRVPTDKGYRYYVDYLMCVQEVTRAERA
                     RIQEELSERLNDADEVIRHTSRLLALTSHQMGLVEVPDERNAEICRIEMLPIGTRRVG
                     VILVDNYGRVRSLVAKLDESLPAEGVQRIGRFLDANFRGVSMQGLHAAVSARTSSFQD
                     EQRRLAEQALRVLALMPPSRQGELILEGTAQLFKQPEFQDMDRARQVFGFFEEHDRLV
                     ELIRSAIRDGEPQRSSIVIGSEVPDDELHEISIVVSPYCVGEQAAGVV"
BASE COUNT          416 a          620 c          670 g          405 t
ORIGIN      
        1 gcgcgtcagc aggccgcgcc gccgcgaccg gcgtggcgca ggccatcgct tcgagcacgg
       61 gcagcccgaa tccttcgtac agggagggaa atacgaatag atcggcggcg gagtagaatg
      121 ccgccagcgc cgcgtgatca tccacgtagc cggtgtgcac cacgcggtcg ccaagctgca
      181 tttcatcccg tacgtgctga tactcttccg ggttccagcc caggggcccg gccagtacca
      241 gctggtgcgg gatctcccgg gcaatcttct ggaatgcgcg gaggagagtc gggatgttct
      301 tccgcggctc aatcatgccc acgaacagga taaatggccc ctgcaccccg caaatccggc
      361 atacctgggc cgcggcttcc tcccggggca tggggccgat gctctcgtcg accgccagcg
      421 gggctacgtg tatcttcgtt gagggcacct ccaggaggcg ctcaatgtcg cgggcagtgg
      481 actctgaata gaccagaatt ccatgtgctt cgcgggcaaa gcgggcgata tggcgcgaaa
      541 acggccccac aatcctcggg ctgcacagat gcggcgcggc cagaaacgac aggtcgtgaa
      601 tggtcacgac ccgcttcgcc acggaggtgg gagggaggaa gtaattcgtt gcgtggtata
      661 catctacgcc atggagcagt gtgtcgactg ccggcttgcc ccagtgttcc cacatcgtgt
      721 aggcgaggcg cgtgggaatc ggcagatgac gatgaaagag cggcctgccc cactgccggg
      781 cgtcaattcg gcgcagaccg gtcgaaaaag ccttcaattc ccaggtatca tccagctgga
      841 gcaggtgctt gaccaagtag tagcagtagt tgccgatgcc cgtccggttt ccggtcacgg
      901 aaccaatgtc tatcccaacc ttcatgcgcg agccagtcct gtttcttcgg gcaatccata
      961 gtaaggccgg tgcggggcaa tgtcaacagg agcgttctcg ggtgcatcgc ttaacttttt
     1021 acatgaaaaa gagttatgag aaacgttctg cgcccgcggc tctcttgaat tcttgacagt
     1081 ggctttaccg tatggtacac tccgcccgtt attagcactc ttctgtcatg agtgctaaat
     1141 gagaggccac acgctcttgg aacaaggcgc cgaacagctc agccagcgtg aacggctcat
     1201 cctgcaggcg gtcgtacatt cgtacatcac cacggcggag ccggtgggct cccgtgccgt
     1261 cgtgaagcgc ttcggcctgg atatcagccc ggccaccgtg cgcaacgtca tggcggatct
     1321 ggaagaagcg gggtacctga agcagctgca caccagttcg gggcgcgtgc ccaccgacaa
     1381 aggctaccgc tactacgttg attatctgat gtgcgttcag gaggtgaccc gggcggaacg
     1441 cgcccgcatc caggaagaat tgtcggaacg gctcaacgat gccgatgagg tgatccgcca
     1501 cacgagccgc ttgctggcgc tgacctccca tcaaatgggg ctggtagaag tcccggatga
     1561 gcgcaatgca gagatctgcc gcattgagat gctgcccatc ggaacgcgcc gcgtcggtgt
     1621 gatcctggtg gacaactatg ggcgggtgcg ctcgctggtg gccaagctgg acgaatcact
     1681 gcctgcggaa ggcgtgcagc ggattgggcg ctttctcgat gcgaatttcc gcggtgtttc
     1741 catgcaggga ttgcatgccg cggtctcggc gcgcacatca tcctttcagg atgaacagcg
     1801 gcgcctggcc gaacaggcac tgcgcgttct ggcactcatg ccgcccagcc gccagggaga
     1861 gctcatcctg gaaggcacgg cgcaattgtt caagcagccg gaatttcagg acatggaccg
     1921 cgcccggcag gtttttggtt tctttgaaga acacgaccgg ctggtcgagc tgatccgctc
     1981 ggcgattcgg gatggagaac cgcagcgttc atccattgtc atcggctcgg aagtgcccga
     2041 cgacgaattg cacgaaatca gcatcgtggt ctcgccctat tgcgtgggcg agcaggcggc
     2101 cggcgtggtg g
//
LOCUS       DAOPBQ010000589         2102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_41515_length_2102_cov_3.280899, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000589 DAOPBQ010000000
VERSION     DAOPBQ010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41515_length_2102_cov_3.280899"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1255)
                     /locus_tag="PKW60_14470"
     CDS             complement(50..1255)
                     /locus_tag="PKW60_14470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK00687.1"
                     /translation="MKVLFLTPRLPYPPAGGDKVTVYSHIRLLSPLVDSIHVLSLIED
                     PAEVQCAREFEAEFPNLTVHTVRLSRNRSRLNAALGLAASSLPLQVHYYRSSVYARRA
                     SELAREIAFDACYVHLVRMAPYARNLPIPVKVLCMTDCLTLRYERSAPYATGMGRHIE
                     RIERKRITPFELQCTEEFNASIVVTEIDRRRLRELGAQGPLALVPFGVDLSRFTLDTA
                     EARDPDCVAFLGNLHSAPNRDAVRYFVEQIWPRIRQHKPGARFQIIGINAPDWVRRLH
                     GNSGVEVAGAVDDVRPYLRRAACTVCPMRIGAGIQTKNLESLALKTPVVSTRIGFEGL
                     DAPEGHGVVVADSPQEFAEHVLGFLSDAAERERQGEAGRKIIENQYDWNTRAPVLYAL
                     LRGEVPREE"
     gene            complement(1290..>2102)
                     /locus_tag="PKW60_14475"
     CDS             complement(1290..>2102)
                     /locus_tag="PKW60_14475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK00688.1"
                     /translation="NINWYLWERYAEASTMGLFVCLFAQHQARLLQRYEPEIWRHFDV
                     ATFCSEEERDYALERQPDAAFHVSPNGVDCSFFQPFETEPEPHTIVFSASMNATQNID
                     AAVFLGEDIFPLIQARYPDSMLYLVGQKPAPEVRRLATDKIVVTGTVDDVRPYAERAA
                     VYVAPIRIGGGTRLKILEAMAMAKPVVSTRVGAEGIRCAAGEHIVLADSPEGFASSVL
                     SLFEHPEERRKLGRAGRELVESTYEWNAIGEKIAHYYDIACANAVRRRGRRA"
BASE COUNT          443 a          645 c          599 g          415 t
ORIGIN      
        1 aatggccaga aaaggcaaaa gatgttcccg ccagctcatg gcatcgatat tactcctccc
       61 tggggacctc gccgcgcagt aaggcataca ataccggagc ccgggtattc cagtcatact
      121 ggttctcgat aattttccgg ccggcctccc cctggcgctc ccgctccgct gcgtctgaca
      181 gaaagccgag gacgtgttcc gcgaactcct gcggcgaatc ggcaacaacc acgccatgcc
      241 cttccggcgc atccaggcct tcgaatccga tgcgcgtgct cactacgggg gtcttcaggg
      301 ccagcgattc caggtttttg gtctggatcc cggcgccgat ccgcatggga cacacggtgc
      361 aggcagcccg cctgagataa gggcgaacat catccacggc gcctgcaacc tccaccccac
      421 tattgccgtg caggcggcgc acccagtccg gcgcattgat tccgatgatc tgaaaccggg
      481 caccgggctt gtgctgacgt atccggggcc agatctgttc aacgaaatag cgcacggcgt
      541 cgcgattggg ggcgctgtgc agattgccga gaaaagcgac gcagtccggg tcacgcgctt
      601 cggcggtatc cagagtgaat cgggacaaat cgaccccgaa cggtaccaaa gccaaaggac
      661 cttgggcgcc aagttcacgc aatcggcggc ggtcaatttc ggtgaccacg atggacgcat
      721 tgaactcctc tgtgcactga agctcgaacg gggtgattcg ttttcgttca attcgctcaa
      781 tatgacgccc cattcccgtg gcatagggcg ccgaccgctc ataacgcaac gttaggcagt
      841 cggtcatgca caggactttc acggggatcg ggaggttcct ggcatagggc gccatccgca
      901 caagatggac atagcaggca tcgaaagcta tctcgcgggc cagttccgat gcccgccggg
      961 catagacgct cgaacggtaa tagtggacct gaagcggcaa agacgaggcc gcaagtccaa
     1021 gcgctgcgtt cagtctggag cggtttcgtg acagacggac ggtatggaca gtcaaattcg
     1081 gaaattcagc ctcaaactcg cgtgcgcact gcacttcggc gggatcttcg atcagcgaca
     1141 gaacatggat ggaatcaacc agcggcgaga gcagccggat atggctgtac accgtcactt
     1201 tgtcgccgcc agcgggcgga tacggcaatc gaggtgtcag aaacaacacc ttcatttgca
     1261 gggtcttccc cctttgctga taccctgcgt catgcgcgcc tcccacgccg tcggacggca
     1321 ttcgcgcacg ctatgtcata ataatgtgcg attttctctc caatggcgtt ccattcgtac
     1381 gtgctttcca cgagctccct gcctgcacgg ccaagcttgc gccgttcttc gggatgctca
     1441 aaaagcgaga gcaccgacga tgcaaaccct tccgggctgt ccgccagcac gatatgctca
     1501 ccggcggcgc agcggattcc ttccgcaccc acccgggttg agaccacggg tttcgccatc
     1561 gccatggcct cgagaatttt gagccgggtt cccccgccaa tacggatggg cgccacatac
     1621 acggccgctc gctctgcata gggccggaca tcgtccacgg tgcccgtgac cacgatcttg
     1681 tctgtggcca gcctgcgcac ttcgggcgcg ggtttctgtc cgacaagata gagcatgctg
     1741 tccggatacc gcgcctgaat cagggggaaa atgtcttccc ccaggaacac cgccgcgtcg
     1801 atgttctgag ttgcattcat ggatgcgctg aacacgatcg tatgcggctc cggttcggtc
     1861 tcgaagggct ggaagaagct gcaatccacc ccgttcggcg atacatgaaa cgcggcgtcc
     1921 ggctggcgtt cgagagcata atcgcgctct tcttcggaac aaaacgtggc gacatcgaaa
     1981 tgtctccaga tttccggttc gtagcgctgc aagagccggg cctggtgttg tgcaaaaaga
     2041 cacacaaaca ggcccatggt tgatgcttcc gcgtaacgtt cccatagata ccaattaata
     2101 tt
//
LOCUS       DAOPBQ010000590         2098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_41622_length_2098_cov_4.370534, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000590 DAOPBQ010000000
VERSION     DAOPBQ010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41622_length_2098_cov_4.370534"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..501)
                     /locus_tag="PKW60_14480"
     CDS             complement(85..501)
                     /locus_tag="PKW60_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00689.1"
                     /translation="MPLAKCPRCNRMFNKIDAPVCLSCMPDEDADYETLRKSMEERPN
                     LIPQEIAEVTGVSLDCILRLIEAGRLATMPDPQTNVTCGRCGAPAISLTKRLCESCLQ
                     KLNSEVAKAQASIKLAKKKKLQIGAGMHIRKWDENY"
     gene            complement(513..1268)
                     /locus_tag="PKW60_14485"
     CDS             complement(513..1268)
                     /locus_tag="PKW60_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00690.1"
                     /translation="MTFTGVKIWSLFIAMGSAMTAASISVWVVYAPASLAVDNPGMIP
                     DEAANEAISILAPGPFPDENTAPLPPGKFEKTAISAQYTPRELPLCDAARYALAYQEN
                     RADDVIRMTHWMQECLKRVQRGSDDPNALAAMYEQLRRSILYRTLEGNRLRPEGVEDK
                     YIFAPGTHIELLAGDNREDCAGDELSCPVRERTWLYVTYPDAAGALRDEEGRPIRSIV
                     VGVAVSPDGQIVKAGVLGNVEIDRERIALDWSR"
     gene            complement(1495..>2098)
                     /locus_tag="PKW60_14490"
     CDS             complement(1495..>2098)
                     /locus_tag="PKW60_14490"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HPK00691.1"
                     /translation="PFVVLGVSQKVREEVCESACQKDGVPILRRCSAGGCVLQGPGSL
                     NFSLVLSYERDPALRNIRESYSHILNKIRKSLVSRGVQTSHEGISDLAIGGRKVSGNA
                     QKRRRQFVLHHGTALYRADSLAMGRYLLEPNDRPEYRGVRPHTQFVTNLPLGPEDLRQ
                     AVREAFNAPDKTVDPLPDEWAETHVLAQNKYQDPSWTFRR"
BASE COUNT          388 a          628 c          628 g          454 t
ORIGIN      
        1 ggggatggcc gggaaggccg gttggcaagg gaaatcctct tgttgcggga ggggtgctcc
       61 gggatcggcc ctgcccgggc gggttcagta gttttcgtcc catttccgga tgtgcatgcc
      121 ggcgccgatc tggagtttct tctttttggc cagtttgatg ctggcctgcg ctttggcgac
      181 ttccgagttg agcttctgca gacagctttc gcaaaggcgc ttcgtgaggc tgatggccgg
      241 ggcgccacag cggccgcagg tcacattcgt ctgagggtca ggcatcgttg cgaggcgccc
      301 cgcctcaatg aggcgcagaa tacagtccag actgacgccc gtcacctcgg caatctcctg
      361 gggaatcaga tttggccgct cctccatgga tttgcgcagc gtctcgtagt cggcatcctc
      421 gtcaggcata cacgagagac agacgggggc atctattttg ttaaacatcc gattacagcg
      481 tggacatttt gccagcggca tagctcctct ccctaacggc tccaatccag agcaatgcgt
      541 tcccggtcaa tttccacatt tcccagcacc cctgctttta cgatttgtcc atccggggag
      601 accgctacgc ccaccacaat gctccggatg ggccggcctt cctcgtcgcg caacgccccc
      661 gcagcgtccg ggtaggtcac gtacagccac gtccgctctc tgacagggca ggaaagctca
      721 tcgccggcgc aatcctcacg attgtcgccc gccagcagtt cgatgtgcgt cccgggcgca
      781 aaaatatact tgtcctcgac tccctccggc cgcagccggt tgccttccag agtgcggtaa
      841 aggatgctgc ggcgcagctg ttcgtacatg gccgccagtg cattggggtc gtcggagcca
      901 cgctgaacac gtttgaggca ctcttgcatc caatgcgtca tgcgaatgac gtcatcggcg
      961 cggttttcct ggtacgcgag cgcgtaacgg gcggcatcgc agagcggcaa ttcacgggga
     1021 gtatattgtg ctgaaattgc ggttttctcg aatttcccag gagggagcgg cgctgtattt
     1081 tcgtctggaa acggccccgg cgccaggatg ctgatcgcct cgttcgcggc ctcgtccgga
     1141 atcatcccgg gattgtccac ggcgaggctg gcaggagcgt aaacgaccca cactgatatg
     1201 cttgcggccg tcattgccga tcccatcgcg atgaacaatg accagatttt gactccggta
     1261 aaggtcatgc catgcaaacc tccccctgcg ctcaggtgca gcatgtcccg ccacaaccgg
     1321 cacgtatcgg caggcgagcc agtttgttgg ttcacctccc ttaatctgcg gcgtttccgg
     1381 tctggaaacc cctcttatat catagcataa tatgcggcaa agtgcgaatt gtcaactaaa
     1441 taccttaaaa atcacgtttt gaggcgcttt tttcgccaaa acaccggaaa aaggtcagcg
     1501 gcggaaggtc caggacgggt cctggtactt gttctgggcg agcacgtggg tttcggccca
     1561 ttcatccggg agtggatcga cggtcttgtc cggcgcgttg aacgcctcac ggacggcctg
     1621 gcgcagatcc tccggcccca gcggcaggtt ggtcacaaac tgcgtgtgtg ggcgcacacc
     1681 gcggtactcg gggcgatcgt tgggttccag gaggtaccgg cccatggcca gcgaatccgc
     1741 tcgatagagg gcggtaccat gatggagcac gaactgcctg cggcgtttct gggcgttgcc
     1801 cgagactttt cggccgccga tcgcgaggtc ggagatgcct tcatgggaag tctggactcc
     1861 gcggctcacg aggctcttcc gtattttgtt caaaatatgg ctgtacgatt cgcggatgtt
     1921 tcggagcgcc gggtcacgtt cgtacgacag gacgaggctg aaattcaggg agcctgggcc
     1981 ctgcaacacg catcctccgg cactgcaacg cctcagaatg ggcacgccat ccttctggca
     2041 ggcgctctcg catacctcct cgcggacctt ttgcgagacg cccagcacaa cgaaaggg
//
LOCUS       DAOPBQ010000591         2095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_41699_length_2095_cov_3.940196, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000591 DAOPBQ010000000
VERSION     DAOPBQ010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41699_length_2095_cov_3.940196"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PKW60_14495"
     CDS             <1..233
                     /locus_tag="PKW60_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00692.1"
                     /translation="LPFVPTVLACIPSSDGKSPCIVIGGEDGPVVVLDASGTPVRQDQ
                     LPDTPVKILFLPEERAVAFGTQDGHVAVCRGF"
     gene            complement(230..826)
                     /locus_tag="PKW60_14500"
     CDS             complement(230..826)
                     /locus_tag="PKW60_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00693.1"
                     /translation="MAYAPEQLAESLRIEAGLLLERSGLAAALHGYGQVHLTGSYALD
                     LMAWRAVDIHLVLYEMWDPLDAFFALGHQIAKQRGVIHMAFGNFLRKPAPGHPGGLNW
                     GVRLLNPGNGEEWMLNIWAVDETHIQQNNGLMQRIREALDEPSRRLILDMKHALLTPE
                     GRTPPLSGIPLCEAVLFEGLRDETAIRAYLRQHGVEGV"
     gene            complement(783..>2095)
                     /locus_tag="PKW60_14505"
     CDS             complement(783..>2095)
                     /locus_tag="PKW60_14505"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPK00694.1"
                     /translation="LVAHRQTDIQSSLSELVQEVQDAGGNVSYDTTRLERYVGTAFEH
                     GIRQIHLHAVGRRVGIHTEPDGRIEPVSAAMKRLPAVEAVIQKHQWQGRIAVNISDEP
                     FIQHEQSYAAVVDQVHEMAPSVRVIEAVESEFFGELDIYVPKLSHLNLWYPRFQEVQR
                     EGKELWYYTCCHPVGRYPNRFLDQSLLKGRVLLWLNYLYGLDGFLHWGLNHFHGDDPY
                     TQEGISHQLPLGDRAVAYPGSSGYLGSLRFSTMRDGLQDFEYMWLLEEGLRALKERAG
                     EDAYWLDPRQRSLELCRRVVQSFYDHTRDPGVLMETRRALAAEIEALAADPLLVVQTS
                     PPENTFVPAGPRNIGVRGLAGPGATVLVNGAPVPVRDSGYFSLPCFLSDNKPTITVSV
                     EKDGVERSVTRSFILSRDQEPTARGPEGKRHGIRSRTTRRIAPH"
BASE COUNT          380 a          641 c          665 g          409 t
ORIGIN      
        1 ggctgccctt tgtgcccacg gtactcgcct gcatcccgtc cagcgacggc aagtccccct
       61 gcatcgtcat tggcggagaa gatgggcctg tggtggtgct cgacgcgtct ggcacgcccg
      121 ttcgccagga ccagctcccg gacaccccgg tcaagatcct tttcttgcct gaagagcgcg
      181 ccgtggcgtt tggcacgcag gacggccatg tcgccgtgtg ccgcggcttt tagacgccct
      241 ccacgccgtg ctggcggaga taggcgcgga tggcggtttc gtcgcgcaga ccttcaaaca
      301 gcaccgcttc gcacaggggg atgccgctga gcggcggtgt gcggccctcc ggggtgagaa
      361 gagcgtgctt catgtcgagg atcaagcggc gggaaggctc gtcgagcgct tcgcggatac
      421 gttgcatgag cccgttgttc tgttgaatgt gggtttcgtc aacggcccag atgttgagca
      481 tccattcttc gccattgccg gggttgagca gacgcacgcc ccaattcagc ccgccggggt
      541 gtccgggagc cggtttgcgc agaaagttgc cgaaggccat gtgaatcacg ccgcgttgct
      601 tggcaatttg gtggcccagt gcgaaaaagg cgtcgagggg gtcccacatt tcatagagca
      661 cgagatggat gtccaccgcg cgccatgcca tgaggtctag cgcgtagctt cccgtcaaat
      721 ggacctgccc atacccgtga agcgcagccg caagaccgga tcgttccaaa agcaggccgg
      781 cttcaatgcg gagcgattcg gcgagttgtt ctggagcgta tgccatgccg ttttccttcg
      841 ggcccacgtg ccgtgggctc ctggtctctg ctcagtataa acgatcgcgt gaccgaacgc
      901 tccacgccgt ccttttcgac gctgaccgtg atggtgggct tgttatcgga caggaaacag
      961 ggcaggctga agtagccgct gtcgcgaaca ggcacgggtg cgccgttgac cagtaccgtg
     1021 gctccgggcc cggccaggcc gcgcacgccg atatttcgcg gtccggcagg cacaaaggtg
     1081 ttttccggcg gcgaagtctg gaccaccagc agcggatcgg ccgccagggc ctcaatttct
     1141 gccgcaagcg cgcgccgggt ttccatcagc acgccgggat cgcgggtatg atcgtaaaat
     1201 gactgcacca cccggcggca gagttcgagc gagcgctggc gcgggtcgag ccagtaagca
     1261 tcctcgcctg cgcgttcctt gagtgctcgc aggccttctt cgagcagcca catatactcg
     1321 aaatcctgca atccgtcgcg catggtgctg aaccggagcg agcccagata gccgctgctg
     1381 ccgggatatg ctaccgcgcg gtcgcccagc ggcagctggt ggctgatgcc ttcctgcgta
     1441 taggggtcgt cgccgtgaaa atgattcagc ccccagtgca ggaatccgtc aaggccgtac
     1501 aggtaattga gccagagcag gacacgccct ttcagaagcg actgatccag gaagcggttc
     1561 ggatagcgcc cgaccggatg gcaacaggtg taataccaga gttccttgcc ttcgcgctgc
     1621 acttcctgaa accggggata ccacaggttg aggtggctca gcttaggcac gtaaatatcc
     1681 agttcgccga aaaattcgga ctccaccgcc tcgatcaccc ggacgctcgg cgccatctca
     1741 tgcacctgat cgaccaccgc cgcgtaggat tgttcgtgct gaatgaaggg ttcatcggag
     1801 atattgaccg cgatgcggcc ctgccactgg tgcttctgaa tcacggcttc gaccgcgggc
     1861 aggcgtttca tggccgccga aaccggttcg atgcggccgt ccggctcggt gtggataccg
     1921 acgcgtcttc caacggcgtg caggtgaatt tgccggatgc catgctcaaa agccgtgccg
     1981 acataccgct caaggcgcgt ggtgtcgtag ctcacgttgc cgccggcgtc ttgaacctcc
     2041 tgcaccagtt cgctcaggga ggactggata tccgtctggc ggtgagccac aagaa
//
LOCUS       DAOPBQ010000592         2094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_41726_length_2094_cov_2.988720, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000592 DAOPBQ010000000
VERSION     DAOPBQ010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41726_length_2094_cov_2.988720"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PKW60_14510"
     CDS             <1..957
                     /locus_tag="PKW60_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00695.1"
                     /translation="FVGVPLLFLPAFAARVFMPGVADANSVYPMVCRQLLPAGLFGMV
                     IAGMFSATMSMLSSDYNAVAAVITNDIYLRLFDPRASQRALVLVGRLATFGVGMIAVA
                     IAVLLSRIENLRDLVQIMAGVFSLLLPPVGIPVFFGFLTRRVSNLGALLGFALGAASG
                     VAAYTLSLLPAESAIVAHVSRVVPQAVLGLYPQGTGLAFLNTVPWLTWITALPTLFGI
                     FCLSALLPDGPVKQKEVDDFLAGVRTGDRPRKASPGASHDAGVAMSIVGLCTGLIGLL
                     LAAAVAVTGHGRDGLYSIGVGLVLALLGFVAWRYGKRVAASS"
     gene            1017..1814
                     /locus_tag="PKW60_14515"
     CDS             1017..1814
                     /locus_tag="PKW60_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPK00696.1"
                     /translation="MRLARILDKNNQVVYAAEQKDGRLLKIEGDISGGESRITDEAVE
                     PSRWLPPLDPTAILCIGLNYRKHAEEGGVPVPEYPVLFMKNPATATGHREPIELPAVC
                     TDEVDYEAELAVVIGKPACNVSKKDALDCVLGYMCSNDVSARIWQSKLGGSQWCRGKS
                     FNTFAPLGPLLVTRDELPNPNALAIKSILNGETMQSSNTKDMIFDVPTLISFLSEDTT
                     LLPGTVILTGTPEGVGWARNPKVVLKPGDTIAVEIEGLGRLENPVTA"
     gene            1909..>2094
                     /locus_tag="PKW60_14520"
     CDS             1909..>2094
                     /locus_tag="PKW60_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00697.1"
                     /translation="MNAFFEYTLGAAVRVADTVLRLLPVPVTAKPLHRLEESANAVRR
                     PALALAAQGGEVGLHAKL"
BASE COUNT          376 a          647 c          657 g          414 t
ORIGIN      
        1 tttgttggcg tgccgctgct atttctgccg gcgtttgccg cgcgggtctt catgccgggc
       61 gtggccgatg ccaattcggt ctaccccatg gtctgccgcc agcttttgcc ggcaggactg
      121 ttcggcatgg tgattgccgg catgttttcc gcgaccatgt cgatgctttc cagtgattac
      181 aacgcggtcg cggcggttat caccaacgac atctacctgc gcctgttcga tccgcgggcg
      241 tctcagaggg ccctggtgct tgtggggcgg ctcgccacct ttggggtagg aatgatcgca
      301 gtggccatcg ctgttttgct ttcccgtata gaaaacctgc gcgacctggt gcagatcatg
      361 gcgggcgtgt tcagcctgct gttgccgccc gtagggatcc ctgtgttttt tggattcctg
      421 acccggcggg tatccaacct gggcgcgctc cttggcttcg ccctgggggc tgccagtgga
      481 gtcgccgcgt atacgctgag cctgttgccc gcggagtccg ccatcgtggc gcatgtgtcg
      541 cgtgtggtcc cccaggcggt actcggcctg tatccccagg gcacgggact ggcatttctc
      601 aacaccgtgc cgtggctcac ctggatcacc gcgttgccga cgctcttcgg gatcttctgt
      661 ctgagcgccc tgttgcccga cggccccgtg aagcagaagg aagtcgacga tttcctcgcg
      721 ggcgtccgta ccggggacag gccccggaaa gcatcccccg gcgcctcgca tgatgccggc
      781 gtggccatga gcattgtcgg actctgcacg ggattgatcg gactgttgct ggcggctgcg
      841 gtcgcggtaa ccgggcacgg cagggacggc ctgtattcga taggcgtggg gctcgtgctc
      901 gccctgctgg gcttcgttgc ctggcgctat ggaaaacggg tcgcggcgtc ttcgtgacat
      961 ggtccggcgg tttgtgtcat gctgcgggag gttcaaaaca aaggaaggac gaggccatga
     1021 ggttggcgcg cattctggat aaaaacaatc aggtggtata tgcggccgaa caaaaggacg
     1081 gccgcctgct caaaattgaa ggcgacatat ccggggggga atcccgcatt acggacgagg
     1141 ccgtcgagcc cagccgctgg ctcccgcccc ttgatccgac cgccatcctc tgtattggat
     1201 taaattaccg gaaacatgcc gaggaaggcg gcgttcccgt gcccgaatac ccggtcctgt
     1261 ttatgaaaaa ccccgcgacg gccacgggcc accgggaacc gatcgagctg cccgccgtgt
     1321 gtacggacga agtggactac gaagctgaac tggccgtggt gatcggcaaa cccgcgtgca
     1381 atgtctcgaa aaaagacgcg ctggactgcg tcctcggcta catgtgcagc aacgacgtat
     1441 ccgcacgcat ctggcagtcg aaactgggcg gctcccaatg gtgccgggga aaaagtttca
     1501 atacctttgc ccccctgggc ccgctcctgg tcacccggga cgaactgccc aaccccaatg
     1561 cactcgcgat caagagcatc ctcaatggcg aaaccatgca gtcatcgaat acaaaagaca
     1621 tgattttcga cgtgccaacg ctgatcagct ttctctcgga agacaccacg ctgctccccg
     1681 gcaccgtgat tttgaccggg acgcccgaag gcgtcggctg ggcccgcaac ccgaaagtcg
     1741 tgctcaaacc cggggacacg atcgccgttg agatcgaagg cctgggccgg ctggaaaacc
     1801 cggtaacggc gtgaacccgg cgcccggaac tcccagccga agcgatacaa ggggccggta
     1861 tgcgccacgg cagatcgtca cgctcccgga gcaaaaaggc tgttgagaat gaacgcgttc
     1921 tttgaatata ccctcggggc tgcggtcagg gtggctgaca cggtgcttcg cctcctgccc
     1981 gtgcccgtga ccgcaaaacc attgcatcgc ctggaggaat cggcaaacgc cgtccgccgc
     2041 ccagcgctgg ccctggccgc ccagggtggt gaagtaggcc tccatgccaa gctt
//
LOCUS       DAOPBQ010000593         2091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_41792_length_2091_cov_2.980354, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000593 DAOPBQ010000000
VERSION     DAOPBQ010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41792_length_2091_cov_2.980354"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKW60_14525"
     CDS             <1..298
                     /locus_tag="PKW60_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00698.1"
                     /translation="RGGFETARRVVPAVEEQRASFLEAMRFRRAEARRRELPASVEQP
                     EGQAFAAIVTRAEAEALGLFAEASHIAQGRPPQAPYPVAGRMAEQAGSQLNFLV"
     gene            516..1445
                     /locus_tag="PKW60_14530"
     CDS             516..1445
                     /locus_tag="PKW60_14530"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPK00699.1"
                     /translation="MRSQRVVRNPGSTTLRSGCSTIWLGLLAKTTDSAGISNLRFDTP
                     EQSLARPGTLLSGVAVGIRQIFPSGEGAWVNALLLLTAAALLAVLIYHVQVHRADGRR
                     ALARDLERRRFLENVLESLAHPFYVIDAKTHEVLMANAAARSPVAAGPVTCHRLMHGL
                     DVPCDGDEHACPLRIVRETRGPAVTEHTHQMGGGEARIFEVRGYPVFDEAGELTQMIE
                     YSLDITERKKVEAERERLIQELETALSEVKTLSGMLPICSSCKKVRDDHGYWKQIEEY
                     ISQRSDAQFSHSVCPDCAVQLYPEWAAKLKRRA"
     gene            1843..>2091
                     /locus_tag="PKW60_14535"
     CDS             1843..>2091
                     /locus_tag="PKW60_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HPK00700.1"
                     /translation="MTLLTIAGIALGLAMDAFAVAIGASIRLGHVSARQVFRFGWHFG
                     LFQALMPILGWYFGSHIEALIAAWDHWIAFALLSCVGGK"
BASE COUNT          381 a          620 c          681 g          409 t
ORIGIN      
        1 ccgaggcggt ttcgagacgg cccgccgagt cgtgccggca gttgaagagc agcgcgcctc
       61 attcctcgag gccatgcgct tccgccgggc agaagcccgg cgccgggagc ttcccgcctc
      121 cgtagagcag cctgaaggac aagcgttcgc ggccatcgtg acccgcgcgg aagctgaagc
      181 gctcgggctt tttgccgagg cgtcgcacat cgcccagggg cggccgccgc aggcgcccta
      241 tccggtagcg ggacggatgg cggaacaagc cggcagtcag ctcaattttc tggtctgaca
      301 gcggcccggc gttctcccgc cgcaataagg aattacggta ctgcgcaccg gcctgccctg
      361 cgcgccgatc cgcttcttct gagaacggcg ctggaagatc ctgccttcct tttatgttcc
      421 atctcaggac agggaatggc tgaataccgc gttcccgaat gaatttgacc gcgccctggt
      481 gtacttttca gtggaatgga gtctcctgac agggcgtgcg gtcgcagagg gtcgttcgca
      541 accctgggag cacgacactg cgcagcggat gctcgacaat ctggctgggg ttgctggcga
      601 agacgactga ttctgccggg atatccaacc tgcggtttga cacacccgaa caatcacttg
      661 cccggccggg aacccttctg tccggcgtgg cggtgggcat ccggcagatt ttcccgtcgg
      721 gcgaaggcgc ctgggttaac gcgctgctgc tgctgaccgc agccgccctg ctggcagtcc
      781 tgatctacca cgttcaggtg caccgggccg atggacgccg cgcgctggcg cgcgatctgg
      841 agcggcggcg ctttctggaa aacgtcttgg aatcgctcgc gcatcccttc tatgtgattg
      901 atgctaagac ccatgaggtg ctgatggcga acgcggccgc gcgttccccc gttgccgcag
      961 ggccggtcac atgccatcgg cttatgcacg ggctcgatgt tccctgcgat ggcgatgaac
     1021 atgcgtgtcc tctgcgtatt gtgcgagaga cgcgcgggcc ggctgtcacg gagcacacgc
     1081 atcaaatggg cggtggcgag gctcgaatct ttgaagtgcg cggctatcct gtctttgatg
     1141 aggcgggcga gctgacgcag atgattgagt attcgctgga catcacagag cgcaagaagg
     1201 tcgaggctga acgggagaga ctcatccagg agctcgagac tgcgctgagt gaggtcaaaa
     1261 cgttgagcgg gatgcttccg atctgcagtt catgcaagaa agtgcgcgac gaccatggct
     1321 actggaaaca aatcgaggag tatatcagcc agcgctccga tgcccagttc agccacagcg
     1381 tgtgcccgga ttgcgcggtg cagctctatc cagaatgggc cgccaaatta aagcggcgcg
     1441 cataaacaca tcgcagcgcg gcccgcgtct gaatggtccg gcgaacccgc gctgcggggc
     1501 gccgcacagg gggaagagga acggacggcc tctaatctga taccattcgg ccaaagcttg
     1561 cctgtacgcg gaaaggagag tctcatgcac gcatggcgca gtgcgctttt ccgggattca
     1621 gtgcggacgt cccgccagcc ggagaaggtg ctcatgaacg cgttcgttcc gaagcgccac
     1681 ggagcccatg agcactcacc agcggcatgt ttctgcctga gcttgcctgg ggaaatgaga
     1741 cccgtcctgc ccggaggcga tcgcctgttc cgctggaatt gggcgcaggc caccgaatgc
     1801 cagggcattc ggtgaaggcc ctcgaaaacg gaacgctgac gcatgacact gctcaccatt
     1861 gcggggattg ccctgggtct ggccatggac gcctttgccg tggccatcgg agcgagtatc
     1921 cgccttgggc acgtatccgc ccggcaggtg tttcgtttcg ggtggcattt cgggctgttt
     1981 caggcactga tgcccatact tggatggtat ttcggttctc atatcgaggc cctgattgcc
     2041 gcctgggatc actggatagc ctttgcctta ctctcgtgtg tgggggggaa a
//
LOCUS       DAOPBQ010000594         2047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_43007_length_2047_cov_2.906124, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000594 DAOPBQ010000000
VERSION     DAOPBQ010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43007_length_2047_cov_2.906124"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PKW60_14540"
     CDS             <1..928
                     /locus_tag="PKW60_14540"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00701.1"
                     /translation="LPNHWHAPMTLEALDAGKHVYVEKPMTHTIREAHRVVTKAAETG
                     LRVQTGVQAMSDDSYSSAWEAIRGGALGPIVHAQTDYVRWYPADKGLWRTDGTAELTQ
                     PEDLDWAMWQKHNPRQIPYDPRIYYDWRCYKDYSGGISTDLFIHRLTRLIKACNLACP
                     VRAVGMGGIFMWPDGRTLPDNFELILEYPAQEGVTPGMTVHVLGTMANKHGIEHCIRG
                     HRATLIFTEDGWRIVEQETGAETLTHKKTGGEDIHLHHKNLQAAIREGVPLNTPPELG
                     LYGVVACTMANISYFEKAMVTYDAKRGKVKKS"
     gene            complement(968..>2047)
                     /locus_tag="PKW60_14545"
     CDS             complement(968..>2047)
                     /locus_tag="PKW60_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00702.1"
                     /translation="QKLRAYFDDLCAWIMGLDVGSNQLKGTSDTAESIFINGNFARVL
                     MASYRITGNEAHRDEALRWCDTFCGIQQRTQTSKGDDAGFWPDIGLRGNIYFGDAGTA
                     AHALASIYPAAGPERQDVWRDAMERMARFVIDGCAEDPQEQGRDATSSWVIREGSDAG
                     ALGCGYYRGKLSVEPYTIATATTGGAFFSELYGITKNPQYRGVAAGAVAWLLTTRKDD
                     GEIPYTLAGNNLDTWPLDTMSYCTEAFVAADLLLEDEALRQSMRRQLEASVQWMINGQ
                     NPDGSWGELRSEDQQRSPRALTLLTWAYPWTAQHDAVARAVNAYCAFLLNPENSAAYG
                     IKELVRTSGFVGLAVADLLQPHCTF"
BASE COUNT          386 a          632 c          651 g          378 t
ORIGIN      
        1 gctgccgaac cactggcatg cgccgatgac gctcgaggcc ctcgatgccg gcaaacacgt
       61 ctatgtcgaa aaacctatga cgcacaccat tcgcgaagcg caccgggttg tcacgaaagc
      121 cgcagaaacc ggccttcggg tgcagacggg cgtacaagcc atgagcgacg acagctacag
      181 cagcgcctgg gaggccatcc gcggcggggc attgggtccg attgtccacg cgcagacgga
      241 ttatgtgcgc tggtaccctg ccgacaaggg cctctggcgc acggacggga ccgcggaact
      301 gacccagccc gaggatttgg actgggccat gtggcagaag cacaatccca ggcagatccc
      361 ctacgatccg cgcatctatt acgattggcg ttgttataaa gactattcag gcggtatctc
      421 gaccgatctg tttattcacc ggctcacgcg cctcatcaaa gcctgtaatc tggcatgtcc
      481 cgtgcgcgcg gtgggcatgg gcggtatctt catgtggccg gatgggcgca ccctccccga
      541 caatttcgaa ttgatcctcg aataccccgc tcaggaaggc gtgacgccgg gcatgacggt
      601 tcacgtactg ggcacgatgg ccaacaaaca cgggatcgag cactgcatcc gcggccacag
      661 ggcgacgctc attttcacgg aagatggctg gcgcatcgtg gagcaggaga ccggcgccga
      721 gacgctgacc cacaaaaaga ccggcggaga ggacattcat ctgcatcaca agaatctgca
      781 ggcggccatt cgggagggcg tcccgctcaa caccccgccc gagctgggcc tctacggcgt
      841 tgtcgcgtgc acgatggcca acatatccta ttttgagaag gccatggtca cctacgacgc
      901 aaaacgcggc aaggtgaaga agtcgtagcg ccgccgcttc cccgacgtca gctgcccgcc
      961 tcagtgctca gaacgtgcag tggggttgca ggaggtcggc cacggccagg ccgacgaagc
     1021 cgctggtccg caccagctct ttgatcccgt aggccgcgct gttctctggg ttgagcagaa
     1081 acgcgcagta ggcgttcacg gcccgtgcga cggcgtcgtg ctgtgccgtc catggatagg
     1141 cccaggtaag cagcgtcagg gcgcgcggac tgcgttgctg gtcttcgctt cgaagctcgc
     1201 cccaggagcc gtcggggttc tggccgttga tcatccattg cacgctggct tcgagctggc
     1261 ggcgcatgct ctggcgcaac gcttcatctt caagcagcag atccgctgcg acgaacgctt
     1321 cggtacagta cgacatggtg tcgagaggcc aggtatcgag attgttgccg gcaagagtgt
     1381 acggaatttc gccgtcgtct tttcgggtcg tgagcagcca ggcgactgcg cccgccgcta
     1441 cgccgcggta ctgcgggttc ttcgtgatgc cgtacagttc ggagaagaac gcgcctccgg
     1501 tggtggccgt ggcaatggtg tagggctcga cgctgagttt cccgcgataa tagccgcaac
     1561 ccagagcgcc ggcgtcactg ccttcccgga tcacccagga cgaggtggcg tcgcgcccct
     1621 gctcctgcgg gtcttcggcg catccgtcga tgacaaagcg cgccatgcgt tccatcgcgt
     1681 cgcgccagac atcctggcgc tcaggtccgg ccgcggggta aatggaggcc agcgcgtggg
     1741 cggctgtgcc cgcgtcgcca aaatagatgt tgccccggag accaatatcc ggccagaacc
     1801 cggcgtcatc gcctttggag gtctgggtcc gctgctgaat gccgcagaag gtgtcgcacc
     1861 agcgcagggc ctcgtcgcgg tgcgcttcgt tgcctgtgat gcgatacgac gccatcagga
     1921 cgcgtgcaaa gttgccgttg atgaagatcg attcggcggt gtcgctggtg cccttgagct
     1981 gattgcttcc cacgtcaagt cccatgatcc aggcgcacag atcgtcgaaa tacgcccgga
     2041 gtttttg
//
LOCUS       DAOPBQ010000595         2041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_43186_length_2041_cov_3.733132, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000595 DAOPBQ010000000
VERSION     DAOPBQ010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43186_length_2041_cov_3.733132"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1254
                     /locus_tag="PKW60_14550"
     CDS             163..1254
                     /locus_tag="PKW60_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00703.1"
                     /translation="MTQQNTRQEVNSTLFIAGFRHAALAAVMLTAGLLASMALIEWVQ
                     RTVAPYHPLAFEEAAREALAQGEYSRVVTICTGNLHYAKNRFPSYGLCRLLRARGYFG
                     LGERDKAFEDLIAAERYWRKWYGRASETERAELKEFATEMAMAALGFGETGMARQLFS
                     LAGTGSGEPLDCLAELHDKLTPQMRANIWPHQERLIVENFMKAGEPVLETWAERTSRT
                     VETAFLDAPSGNSGDPGRLLRITASLAPENGMSWYSIPFFMPIPDSPCRFRAVIDLNA
                     GPPPSLLLGFAHPATGQGGTVFGETPEKLADGSYAAEVLLPGRNSGKTPDYVVRVGFA
                     LPMEACECIIKRIELFPDGGAETGTDGQG"
     gene            1241..>2041
                     /locus_tag="PKW60_14555"
     CDS             1241..>2041
                     /locus_tag="PKW60_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00704.1"
                     /translation="MGKGKRQKVKSQPAPRAAARSAKAVLRVDGVLRFFFALLLLAVI
                     LALYPYTQDATGPIKYLLLAWGAFIASSLLAILGTLQKTPWQTPRVFLPILLAFVGLN
                     LVSALLSDHAGHAMVTWRRFGMLFIVYLIATQIYRKPRHISALMLIACIGVTFSTLYA
                     IFQYIGLDPFPWESKDSPIYRELPGTFGNPNYAAHTMILCIAMSAFLATRRKKRWTAA
                     FGILFLVHMYFTGQRGGLVGLALGAALVLGARTLRKYVRSPRYDITAAL"
BASE COUNT          387 a          651 c          585 g          418 t
ORIGIN      
        1 tcgcaaatgg caggacgata ccatccccgg taagatctta gatggcgcga ttcgcacact
       61 tgattgaggt cgcgcggttt gggatgatga agcccatcca ttcgcgcggc aggcggcccg
      121 tttcgggctg gcccggatca cagcgccgga atccaggaaa cgatgacaca acagaacaca
      181 cgacaagagg ttaactcaac cttgtttatc gccggattca ggcacgccgc cctggccgcc
      241 gtgatgctga cagcgggtct gctggcttca atggcactga tcgagtgggt gcagcgaact
      301 gttgcgccct atcatccgct ggcgtttgaa gaagcggccc gcgaagccct ggcccagggc
      361 gagtattccc gggtagtcac tatctgtacg ggcaacctgc actacgcgaa gaacagattt
      421 ccaagttacg ggctctgccg cctcctccgc gcccggggct atttcggcct cggagaacgc
      481 gacaaggcct tcgaggacct gatcgcagcg gagagatact ggcggaagtg gtacgggcgg
      541 gccagcgaga ccgagcgagc cgaactgaag gaattcgcca cggaaatggc catggcggcc
      601 cttggtttcg gtgagacagg catggctcgc cagcttttct cgcttgcagg taccggttcc
      661 ggcgaaccac tcgactgcct ggcagaattg cacgacaagc tcacgcccca gatgcgcgcc
      721 aacatctggc ctcaccagga acggctgatc gtcgaaaatt tcatgaaagc cggcgagcct
      781 gtactcgaaa cctgggccga gcgaaccagc cggacggttg agactgcgtt cctggatgcg
      841 ccctctggca attcaggcga ccccggccgc ctcttgcgca tcacggccag cctggcgcct
      901 gaaaacggca tgagctggta cagtatccct tttttcatgc cgatccccga ctccccgtgc
      961 agattccggg ctgttatcga tctcaacgcc ggcccgcccc cgagccttct tctcgggttc
     1021 gcccatcccg caacgggcca gggcgggaca gtctttggcg aaacacccga gaagctcgcg
     1081 gacggcagtt atgcggcgga agtcctgctt ccgggccgaa attcgggtaa aacccccgac
     1141 tacgtcgttc gtgtcgggtt cgcgctcccg atggaagcct gcgagtgtat cataaagcgc
     1201 attgagcttt tccctgacgg cggggcagaa acgggaaccg atgggcaagg gtaagcgtca
     1261 gaaggtaaag agccaacccg cgcctcgcgc ggcagcacgg agcgccaaag cggtgctgcg
     1321 ggtggacggc gttctgcgtt tcttcttcgc gcttctcctc ctggccgtca tcctggcgct
     1381 ctatccgtat acccaggacg caaccggccc gattaaatat ctcttgctgg cctggggcgc
     1441 ctttattgcc tcatccctcc tggccatcct ggggacactc cagaaaacgc cctggcagac
     1501 gccgcgggtc ttcctcccca ttctcctggc atttgtcgga ctgaacctcg tgtcagcact
     1561 gctttcagac catgccgggc atgcgatggt gacatggcgc cgtttcggca tgcttttcat
     1621 cgtgtatctc atcgcgacgc agatctaccg taaaccccgc cacatctccg cgctgatgct
     1681 gatcgcctgt attggcgtga ccttttccac tctctatgcg attttccaat acattggcct
     1741 cgatcccttc ccctgggaga gcaaagactc accaatctat cgtgaattgc cgggcacctt
     1801 cggcaacccg aattatgccg cccataccat gatcctgtgt atcgcgatgt cggcgttcct
     1861 tgccacacgc cgcaagaagc gttggacagc agccttcggc atcctgtttc tagtgcatat
     1921 gtacttcact ggacagcgcg gcggcctggt cggccttgcc cttggcgctg cgctggtcct
     1981 cggcgcgcgt acgctgcgaa aatacgtacg ttctccccgc tacgacatca ctgcggcgct
     2041 g
//
LOCUS       DAOPBQ010000596         2041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_43197_length_2041_cov_2.876133, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000596 DAOPBQ010000000
VERSION     DAOPBQ010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43197_length_2041_cov_2.876133"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKW60_14560"
     CDS             <1..346
                     /locus_tag="PKW60_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013072892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPK00705.1"
                     /translation="LAAVEGRSKRLGACADTGHWPRSGVDPLEALKKLEGRIISFHFK
                     DLNEFGKREAHDVPWGTGICNVEGLLKEIKRQGLKDVVFSIEYEYNWDNSVPEIAQCV
                     AYFDKVAAELAD"
     gene            429..1829
                     /locus_tag="PKW60_14565"
     CDS             429..1829
                     /locus_tag="PKW60_14565"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPK00706.1"
                     /translation="MNRRAFLHTCALGGAATLAGSQARGEEAAASRPNILFFLIDDQR
                     HDTLGIAGHRIARTPTIDSLARRGVRFENAFVTTSICAASRASILTGLTERTHGFTFG
                     TPPIRKSHIETSYPAVLRAAGYRTAHVGKLGVKMDAGPEETLFDFFSERDRPYLIKQP
                     DGTIRHIDEINMEHAIQFLDSCSVQQPFCLSVCFSSTHAEDSDKENHYPCIDAVKGMF
                     EDTIFPPPLLDSPEIFDAHPAFLRESMNRERFFWRWDTPEKYQKNMRAYFRLLAGVDY
                     MIAKITAKLEEQGLADNTVIIYASDNGYYMAERGFAGKWSHYEESLRVPLVIYDPRLP
                     QNLRGRTIGPMALNIDIPATVLDIAGAAIPERYQGRSLLPWLNGESPGDWRREFFCEH
                     RMKHELIPTWEGIRGERYVYARYDGQTPPYEFLHDLEKDPYQLHNYAQDDACAATLAE
                     YRSRCDAALKRYQDAS"
     gene            complement(1885..>2041)
                     /locus_tag="PKW60_14570"
     CDS             complement(1885..>2041)
                     /locus_tag="PKW60_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPK00707.1"
                     /translation="MLGEERDLEVDGGIDQHTAPQVIAAGANVLVAGSYVFGSESYLE
                     AIESLRV"
BASE COUNT          429 a          653 c          573 g          386 t
ORIGIN      
        1 cctggcggcc gtggaaggtc gaagcaaacg cctcggcgcc tgcgccgaca ccggtcactg
       61 gccccgttcg ggcgtcgatc ccctggaagc cctgaaaaaa ctcgaagggc gtatcatcag
      121 cttccatttc aaggatctga atgaatttgg caagcgcgaa gcccacgacg tgccctgggg
      181 caccggcatc tgcaacgtcg aaggcctctt gaaggagatc aagcgtcagg gcctgaagga
      241 tgtcgtgttt tccatcgaat acgagtacaa ctgggacaat tccgtgcctg agatcgccca
      301 atgcgttgcc tactttgaca aagtcgccgc cgaactggcg gattgacctg aatgtctcat
      361 gccgccccgg gagcctctac tcccggggcg cgttcttgct tccaacgggc gccagggggg
      421 ttaaagcaat gaaccgacga gctttcctgc atacctgtgc actcggaggc gcggcaaccc
      481 tcgcaggcag ccaggcacga ggggaagaag ctgcagccag ccggcccaat atcctcttct
      541 tccttatcga cgaccaacgt catgacaccc tgggaatcgc aggccaccgc atcgcccgga
      601 cgcccacgat tgacagcctg gcccgccggg gggtccgttt tgaaaacgcg ttcgtgacga
      661 cgtcgatttg cgccgccagc cgtgcgtcca tcctgacggg cctcaccgag cgtacccacg
      721 gattcacgtt cggcacgccg cccatccgca aaagccacat cgagaccagc taccccgctg
      781 ttctgcgcgc cgcgggatac cgcactgccc acgtcggcaa gctgggcgtt aaaatggatg
      841 ccggcccgga ggagaccctg ttcgacttct tcagcgagcg tgaccggccg tacctgatca
      901 aacaaccgga cgggaccatt cggcatatcg acgagatcaa tatggaacac gccatccagt
      961 ttctggactc ctgttccgtt caacagccct tttgcctttc cgtctgtttc agctcaacgc
     1021 acgcggaaga cagtgacaag gaaaaccact acccctgtat tgacgcagtc aaaggcatgt
     1081 tcgaagacac cattttcccg ccgccactgc tggacagccc cgagatcttc gacgcccatc
     1141 ccgcgttcct gcgcgagtcc atgaaccgcg aacgcttttt ctggcgctgg gatactccag
     1201 aaaagtatca gaaaaatatg cgggcatact tccgcctttt ggcgggcgtt gattacatga
     1261 tcgcaaagat cacggcgaag cttgaggaac agggcctggc tgataatacc gtcatcatct
     1321 acgcgtccga caacggctat tacatggccg agcgtggatt tgccggaaaa tggtcgcatt
     1381 acgaagaatc gttgcgcgta cccttggtta tttacgatcc gcgcttgccg cagaatctgc
     1441 gcggacgtac catcggcccc atggccctca atatcgacat ccccgccacg gtgctcgaca
     1501 tcgcaggcgc ggccataccg gagcgctacc aggggcgcag tctcctgccc tggttgaacg
     1561 gagagtcacc cggggactgg cgccgcgagt ttttctgcga acatcgcatg aaacacgagc
     1621 tgattcccac ctgggagggt attcgcggcg agcggtacgt ctacgcccgt tacgacggcc
     1681 agacgccccc ctatgagttt ctccacgatc tcgaaaagga cccttatcag ctccacaact
     1741 atgcccagga cgatgcctgc gctgcgacac tcgcggaata ccggagccgg tgcgatgccg
     1801 cgctgaagcg gtatcaggac gcctcctgaa cgagaggccc cgggctgcgg agtgtgtgga
     1861 aaaggtgcgc gatcagtcgg gaattcacac gcgcaggctt tcgatggcct cgagatagct
     1921 ttcgctcccg aacacatatg agcccgcaac aagcacgttg gcgccggccg caatcacctg
     1981 gggtgcggta tgctggtcga tcccgccgtc cacttcgagg tcgcgttcct cacccagcat
     2041 g
//
LOCUS       DAOPBQ010000597         2038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_43282_length_2038_cov_2.913767, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000597 DAOPBQ010000000
VERSION     DAOPBQ010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43282_length_2038_cov_2.913767"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PKW60_14575"
     CDS             <1..978
                     /locus_tag="PKW60_14575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK00708.1"
                     /translation="IFSAGYPEDKLEVVAVNDGSTDDTLTHMLAAQSRHPRLVVVDFE
                     RNRGLCHGWGVATLIARGEFMVCVDSDTFIFPGALQKLMQGFIDPSVGGISGHCDVAN
                     ASVNLLTRMQDVRYYFSYKIMKAAESVFGTVSCLPGCFSAYRRVCVLHVLDEWMNATV
                     MGEHGNFADDRSLTNKILRDYKILYDDEALATTIAPENWYQYTRQQARWMRSYLREIV
                     KTGKFMWRKHPVPALSWYAMMWMPIFEPVVMLQALVIGPVLAGHVTASYTLGVVAITA
                     VWSLHFWASTGRRWWWAGFVFTASYIAFYSWQIYWALITLRGRKWGTRG"
     gene            975..>2038
                     /locus_tag="PKW60_14580"
     CDS             975..>2038
                     /locus_tag="PKW60_14580"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPK00709.1"
                     /translation="MNKTDEKETGVLNGGTGAATPAEPELNDGWERPVAVEKDRNVRA
                     WLIFLGVLCFLVALWTPLRALYYRHGYPKTDVEIVRDADTFVAVAYYGVSSDATGDSR
                     DISKETFAEQLRLLRENGYTPITLREVQAFYKEGKPLPRKAILMTFEQSRKSSYFEIR
                     DLLHLYKWKAVMGVTTAPMHTKDAQVLLWPYLRDMLTMGSWELAAQSEHGFDFIPTSP
                     AGRTGSFFANPQWLEDRRRYELPEEFNRRLQDDHEKVIREFEKEVGGKPIAFFFPYGD
                     YGQYEERARVVRVTNMHQVGAHYDLGFILGQLALNTRHSDPRRLNRLLVDPAWSPREF
                     IRKLETFWPVEPGCDKEHEA"
BASE COUNT          383 a          576 c          655 g          424 t
ORIGIN      
        1 atcttctccg ccggttatcc ggaggacaaa ctggaggtgg tggccgtcaa cgacggctcg
       61 acggacgaca cgctgacgca catgctggcg gcgcaatcgc gccaccccag gctggtggtg
      121 gtggatttcg agcgcaaccg ggggctgtgc catggatggg gcgtcgcgac gctgatcgcg
      181 cgcggcgagt tcatggtctg tgtggattcc gataccttca tcttccctgg ggcgctgcaa
      241 aagctgatgc agggctttat cgatccttcg gtcggcggga tctccggcca ttgcgatgtg
      301 gcgaacgcca gcgtcaatct gctgacgcgc atgcaggatg tgcgctacta cttctcttac
      361 aagatcatga aggccgcaga gagcgtattc ggcaccgtga gctgcctgcc gggctgcttc
      421 tcggcttacc ggcgcgtctg tgtgctgcat gtgctggacg aatggatgaa tgccacggtc
      481 atgggcgagc atggcaactt tgcggatgac cgctcgctga ccaacaagat cctccgggac
      541 tacaagatcc tgtacgatga cgaggcgctg gccacgacca tcgcgcccga aaactggtat
      601 cagtacactc ggcagcaggc acgctggatg cgttcttatc tgcgcgaaat cgtcaaaacc
      661 ggtaagttca tgtggcgtaa gcatccggtg cccgcgctct cgtggtacgc gatgatgtgg
      721 atgccgattt ttgaaccggt cgtcatgctt caggcgttgg tgatcggccc ggttctggcc
      781 gggcatgtca cggcttcata cacgctgggc gtggtggcga tcacggcggt ctggtcgctg
      841 catttctggg cctccaccgg ccgtcgctgg tggtgggcgg gatttgtgtt tacagcgtcc
      901 tatattgcgt tctatagttg gcaaatttac tgggcactga tcaccttgcg cggtagaaag
      961 tggggaacac gaggatgaac aagacggatg agaaagagac cggcgtcctg aacggcggca
     1021 ccggcgcggc gactcccgcc gagcctgagc tgaatgacgg atgggagcgt ccggtcgcgg
     1081 tcgagaagga tcgcaacgtg cgcgcctggc tcatttttct cggcgtgctc tgtttcttgg
     1141 tcgcgttgtg gacacccttg cgtgcgctct attaccgcca tggttatccg aagacggatg
     1201 tcgagattgt gcgcgatgca gacacgtttg tcgccgtggc ctactacggc gtcagcagcg
     1261 atgccacggg agattcgcgg gacatttcga aggaaacgtt cgccgaacag ctccggctgc
     1321 tgcgcgagaa cggatacact cccattacgc tcagggaggt ccaggccttc tacaaggagg
     1381 gcaagcccct gccgcgcaag gccatcctca tgacctttga gcaatcgcgg aaaagctcgt
     1441 atttcgagat ccgtgatctg ctgcacctct acaagtggaa agcggtgatg ggcgtgacga
     1501 ccgcccccat gcacaccaag gacgctcagg tgctgctgtg gccctatctg cgcgacatgc
     1561 tgacgatggg ctcgtgggag ctggccgcgc agtcggaaca cgggttcgat ttcatcccga
     1621 ccagccctgc cgggcgcacg ggttcttttt tcgccaaccc ccaatggctg gaggaccgtc
     1681 ggcgttacga attgcccgag gagttcaacc ggcggttgca ggatgaccat gagaaggtga
     1741 tccgggaatt tgaaaaagag gtcggcggga aacccatcgc cttctttttc ccgtatggcg
     1801 attacgggca gtatgaagaa cgggccagag tcgtgcgggt gaccaacatg catcaggttg
     1861 gtgcccacta cgatctgggt ttcatcctcg gccagttggc gctcaacacg cgtcatagcg
     1921 atccgcgccg cctcaaccgg ctgctggtcg atcccgcctg gagccccagg gagttcatcc
     1981 gcaagctgga aaccttctgg ccggtggagc ccgggtgtga caaggagcac gaagcctg
//
LOCUS       DAOPBQ010000598         2037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_43308_length_2037_cov_3.785570, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000598 DAOPBQ010000000
VERSION     DAOPBQ010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43308_length_2037_cov_3.785570"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            453..1430
                     /locus_tag="PKW60_14585"
     CDS             453..1430
                     /locus_tag="PKW60_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPK00710.1"
                     /translation="MTPKIAAALEGFSGSPDRYCPSGYRDPLRMQERLAALSKIKAIK
                     AVEVAQTDVSPEMPAKELKRLLKDYGLACSGVSANLAYDRRFALGAFGHQHPKTRNAA
                     IDEGRKAADLARQLGATEITLRLYSDGFDYAFHIDYAVHWNTLISSIKTIAKYASPDV
                     NVAIAYKGAEPRKFLTVSTVGKALSLCQEIAMKNVGVALNFSHAMMAGENPAEAIAFL
                     SRSNKLFQLYLNDSYHSADDMLISGSVHLWETMEALFYLKVTKYKGYLTLDMQPKRID
                     PIQACQIAAGNVAILWKKLEKIDTTELRKAQKTLDAVESQKIIRRAMLQ"
     gene            1769..>2037
                     /locus_tag="PKW60_14590"
     CDS             1769..>2037
                     /locus_tag="PKW60_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPK00711.1"
                     /translation="MLVDRRGYVHACIVGDARSVFLPDLSTYRQSRARLCGLRLIHTH
                     LNREPLTDDDFTDLALLRLDCIVAIEVLEGGLPGTLHVAHLLPGR"
BASE COUNT          447 a          582 c          574 g          434 t
ORIGIN      
        1 tcgcctttcc cccaggcgta ccgcagtggc agtcagaaat gactggttcc caccaccggc
       61 gagtgcgtga acggcagtcc ccccgctgcc cgcactcgcc tcctcttttt ttgctaagtc
      121 ttttgattcc ttgcgattac cgggcaaccc cgcgaaatac accgcgatcc gcgaggaaac
      181 agggctttcc tgtatgatct gggcgcaggc gcgggggcag atgccgataa tcctgcacaa
      241 aattcggtgt tttctcccaa ctattgacaa tttggctccg aatttctaaa atattcccgt
      301 gtgcgtgccg caattcgccg aggggctaca tgcgtgtttc acggctcaca ttgttgccgc
      361 aggtgggaat tcatttgccc tcgtatacca aaattcagcc ttcagcatac cacaactgag
      421 cgcacacaga agcgtcacaa caggagaatg aaatgacccc caaaattgca gccgccctgg
      481 aaggattctc cggttcgccg gatcggtact gcccgagtgg gtaccgcgac ccgctccgaa
      541 tgcaggaacg cctggcagcc ctgtctaaga ttaaggcgat caaagccgta gaagtcgccc
      601 agactgatgt ttccccggag atgcctgcga aagagctgaa acgcctgttg aaagactacg
      661 gccttgcctg ttcgggtgtg tcggcgaacc tcgcgtatga tcggcgtttt gcgctgggcg
      721 cctttgggca tcaacacccg aagacccgta atgcggccat tgacgagggt cgaaaagccg
      781 cggatctggc gcgccagctc ggagcgacgg aaatcacact gcgcctgtac agtgacggct
      841 tcgattatgc gtttcacatc gactatgccg tgcactggaa caccctgata tcgtccatca
      901 agacgatcgc taaatatgca tcgccggatg tgaacgttgc cattgcttac aaaggggcgg
      961 aaccccgcaa gtttctcacc gtgtcgacgg tggggaaagc actgtcgctg tgccaggaaa
     1021 tcgcgatgaa aaacgtgggg gttgctctga acttttcgca tgcgatgatg gccggcgaga
     1081 atccagccga agcgattgcc tttttgtcgc gctccaacaa gctgtttcaa ctctatttaa
     1141 acgacagcta ccacagtgcc gatgatatgc tgatttcggg cagtgtccac ctctgggaga
     1201 cgatggaggc gctgttttat ctcaaagtca cgaaatacaa gggttatctg acgctggaca
     1261 tgcagcccaa gcgcatcgat ccgatccagg cctgccagat tgcggcaggt aatgtcgcga
     1321 tactctggaa aaagctcgag aaaatcgata cgacagaact gcgaaaagct cagaagacgc
     1381 tggatgccgt cgaaagccag aagatcatcc ggcgcgccat gttgcaatag taaggatcag
     1441 aaagcaagcg gcgcagcatt cccgggccgg ccgatagcgt ccgggcattt cgttgcgcag
     1501 caacgcggac tgcggagttt tttgtatact cccctgaatc ttgctagtcc agcggagcgc
     1561 ccgtctgccg gatgggcgac ggaagccatt cgaccaagcg agggatcctg ctatccgctt
     1621 aacaggcaat atccaaggcc ttaaagcctc ttctctaaag cggttacaaa gactgggcaa
     1681 ccggaggacc cgccctgagc ggctggtcag cccggaattg gcccgggcat taacggagat
     1741 ttccgcggag atccaacggc aggtgggcat gctggtggat cgccggggct acgttcacgc
     1801 gtgcattgtc ggagacgccc gttccgtctt tctgccggac ctttccacgt atcgccagag
     1861 ccgcgcccgg ctgtgcgggt tgcgcctgat tcatacgcat ctgaaccgtg agccacttac
     1921 cgatgatgat ttcacggatc ttgcgctgct gcggcttgat tgcatcgtgg cgattgaagt
     1981 ccttgaaggc ggcctgccgg gaacgcttca cgtggcccac ctcttgcctg gccggga
//
LOCUS       DAOPBQ010000599         2020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_43801_length_2020_cov_2.730789, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000599 DAOPBQ010000000
VERSION     DAOPBQ010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43801_length_2020_cov_2.730789"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKW60_14595"
     CDS             <1..198
                     /locus_tag="PKW60_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00712.1"
                     /translation="QQGPDFWTVEIAIPWRALNMAPPESGSAIRINLCRERYTEREWT
                     LWSQTVYHFLEPEHFGTFIFK"
     gene            398..1552
                     /locus_tag="PKW60_14600"
     CDS             398..1552
                     /locus_tag="PKW60_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK00713.1"
                     /translation="MSQSLSMHRMIWREIQRSKWSTLLCFCIVVLATSLLAIIIAVGR
                     SSVDATRVQMKDMGFNLLITPPGADLAQYQALNFDGPDMPEEYVDKLAASTVLAQHFV
                     GKYQKTLQVDGKTVVLTGLRREESQVNAYRKPMPTAYDVPPGKVFAGAAAAKALELTP
                     GSTVTILGRPFEVAGVLEPKGAIPEDIRLFLHLSDVQALLGVDGRINAIDALACMCPV
                     DTKDILNALENSIRSVLPDVGVEPYRDILLARHEQRGMVYRLQLATVAIVVAGAATAI
                     WGLTYLNVRNRRSEIGVFRALGIRGGRIATLFIAKIAFYSLAGGIVGCALGYILAPAV
                     VVTSRVLLPDISSLVLIAGMTPLVAILFGLPSIVAGLLHDPVDVLREAAA"
     gene            1549..>2020
                     /locus_tag="PKW60_14605"
     CDS             1549..>2020
                     /locus_tag="PKW60_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK00714.1"
                     /translation="MIQFDGVSKQFRKDGALVHALDSVSFQTEPGQLALVRGPSGSGK
                     TTLINLAAGLTRASQGSIRVAGTCLDTMSPRERDALRARDVAVVFQLFHLVPYLTAFE
                     NVLLPTLAPTQPGNAAEHARELLAELGLESRANHYPDEMSAGERQRCALARALLK"
BASE COUNT          387 a          664 c          590 g          379 t
ORIGIN      
        1 cagcagggtc ccgatttctg gaccgtagag atcgccatcc cctggagggc cctcaacatg
       61 gcgcctcccg aatccggcag cgcgatacgc atcaacctct gccgggaacg ctacacagag
      121 cgcgaatgga ccttatggtc gcaaaccgtc tatcatttcc tcgaacccga gcatttcggg
      181 acgtttattt tcaagtgatg gcgtgacaaa gcagtacccc gggaagcctg tattctcttc
      241 aacgcaggat gtcccgcgga gcaatcgttg ttcaaggtgg gctggctgct catgggcgaa
      301 gccgtattcg ccgccccggc gccagctctc tgccggcccc aggttcggat tggaaaatgc
      361 cagcgtggcg cctgcaccac acacgaacgg aggaaccatg agccaaagtc tgtcaatgca
      421 ccgcatgatc tggcgcgaga tccagcggtc caagtggagc accctgctct gtttctgcat
      481 agtcgtgctg gccacaagcc tgctggccat cataattgcc gtgggacgaa gctctgtcga
      541 cgccacccgc gtccagatga aagacatggg cttcaatctt ttgatcacac caccgggcgc
      601 agatctcgcc cagtaccagg cgctcaactt cgacggcccc gatatgccgg aggagtacgt
      661 cgataagctg gccgccagca ccgtgctggc ccagcatttc gtcgggaaat atcaaaaaac
      721 gcttcaagtc gatggcaaaa ccgtcgttct gaccgggctc cgccgtgaag aatcccaagt
      781 gaacgcctat cgcaaaccca tgccgaccgc atacgacgtc ccgccaggga aagtgttcgc
      841 gggggcggct gccgccaaag ccctggaact gacgcccggc agcaccgtga ccatcctcgg
      901 ccggccgttc gaggtcgccg gcgtcctcga acccaaaggc gccatccccg aagacatccg
      961 cttgtttctg catctgagcg atgtccaggc ccttctcggg gtagacggcc gcatcaacgc
     1021 cattgacgcc ctcgcctgca tgtgccccgt cgacaccaaa gatatcttga acgcgctcga
     1081 aaacagcatt cgctccgtgc tccccgatgt cggcgtggag ccgtaccgcg acatcctcct
     1141 ggctcgtcac gagcagcgcg ggatggtcta ccggctgcaa ctggcgaccg tggcgatcgt
     1201 ggtcgcgggc gcagcgacgg ccatctgggg attgacctat ctaaacgtac gcaaccgccg
     1261 ttcggaaatc ggcgtgttcc gcgccctggg catccgcgga ggccggattg ccacgctttt
     1321 catcgccaag atcgccttct acagcctggc gggagggatc gtgggatgcg cactgggcta
     1381 catcctggcg cccgctgtgg tcgtgaccag ccgcgtgctg ctgccagaca tcagctcgct
     1441 tgtcctgatt gctggcatga cgccgctggt ggcaatctta tttggcctgc cgtctatcgt
     1501 ggcgggcctg cttcacgatc cggtcgatgt attgcgggag gctgcagcat gattcagttt
     1561 gacggcgtat caaaacaatt tcgtaaagac ggggccctgg tgcatgccct cgacagcgtt
     1621 tcatttcaga cagagccggg gcagctggca ctggtccgcg gaccctccgg aagcggaaag
     1681 accaccttga taaatctcgc agcaggactc acccgcgcct cacagggcag cattcgcgta
     1741 gccgggacct gcctcgacac gatgtcgcct cgtgagcgtg acgcattgcg tgcgcgcgac
     1801 gtcgcagtcg tgttccagct ctttcacctc gtaccctacc tgaccgcgtt cgaaaacgtg
     1861 ctcctgccaa cccttgcgcc gacacagcct ggcaacgcgg ccgagcatgc ccgggagctg
     1921 ctggccgagc tgggcctgga gtcccgggca aaccactacc ccgacgagat gtcggcgggc
     1981 gagcgccagc gctgtgccct ggcccgggct ctgctcaaac
//
LOCUS       DAOPBQ010000600         2013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_43991_length_2013_cov_4.557201, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000600 DAOPBQ010000000
VERSION     DAOPBQ010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43991_length_2013_cov_4.557201"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PKW60_14610"
     CDS             complement(<1..1128)
                     /locus_tag="PKW60_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00715.1"
                     /translation="MYAMGCLLAVAFAGIWSGVPAPPAIFLGPEASAIEQGASTDLQR
                     LLHAATGTLYPVKTIDTVPADAKGIVIGTPSSLPKAGQSWPFGLEEPGADGYILYSDT
                     DTNSLVFVAGQTATAAQHGVYGLLETLGFGFYTSQETLPDAFSDIPALRLPRFNASVT
                     PVFKVRGTLPFYDYLMGHSAWEIRDYKAYMDALARMRMNTVTFYVRDDQPFAAYEFDG
                     VLTGGEPPAGMASAKWRTEPVPTEEFYAGTGRFFARDTFGAANALVEEQQPAITQGKA
                     VLREAIAYARSRGLDVGLGIEVRGDIMDLAVSDRFEARLRSILADYPDLTSLWLWQPE
                     GQALYPGDEPAPRSAWASYAQRWDDVFGDSMDARQRAEAVRM"
     gene            complement(1710..>2013)
                     /locus_tag="PKW60_14615"
     CDS             complement(1710..>2013)
                     /locus_tag="PKW60_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPK00716.1"
                     /translation="AEKLAERIQQLGGTPLLNPAEWMKKTNCGYEEPANPNTVVLLEQ
                     NIKGEQCAIETYKKLIDFVRGKDDITYHLIFDILKDEVEHEEDLEAILEDMKTPVK"
BASE COUNT          415 a          616 c          582 g          400 t
ORIGIN      
        1 catccggacg gcctcggccc gttgccgcgc gtccatgctg tcgccaaaga catcgtccca
       61 gcgctgcgca tacgacgccc acgcagagcg cggagcgggc tcgtcgccag ggtacaaagc
      121 ctggccttcc ggctgccaca gccacaggct ggtcaagtcc gggtagtcgg ccaggatgga
      181 acgcaggcgc gcttcgaaac ggtcgcttac ggcgagatcc atgatgtcgc cgcgtacctc
      241 aatcccaagt cccacgtcca atccccggct tcgggcatag gcaatggctt cgcgcaatac
      301 cgctttgccc tgggttatgg ccggctgctg ttcttcaacg agggcattgg cggcgccaaa
      361 ggtgtcgcgg gcaaaaaaac ggcctgtacc ggcatagaat tcttccgtcg gcacgggttc
      421 ggtgcgccac ttggcgcttg ccataccggc cggcggttcc ccgccggtca ggacgccatc
      481 aaattcatac gcggcgaacg gttgatcatc acgcacgtaa aaggtcacgg tgttcatccg
      541 catgcgtgcc agcgcatcca tgtaggcctt gtaatcgcga atttcccagg cagaatgccc
      601 catcaggtag tcataaaatg gcagggtgcc gcgcactttg aaaacaggcg tgaccgacgc
      661 attgaaccgt ggcagccgca gtgcagggat gtctgaaaac gcatcgggca acgtttcctg
      721 cgaggtatag aagccgaatc ccagggtttc cagcaatcca tacaccccgt gttgcgccgc
      781 cgtggcggtc tgtcccgcaa cgaataccag gctgttggtg tccgtgtccg aatagagaat
      841 gtagccgtcc gcccccggtt cctcgaggcc aaacggccag ctctgcccgg cttttggaag
      901 ggacgaaggg gtcccaatca cgattccctt tgcatccgcg ggcacggtgt ctatcgtctt
      961 gacaggataa agcgttcccg tggcggcatg aagaaggcgc tgcaaatcgg tcgacgcgcc
     1021 ttgttcgatt gccgaagcct ccgggccgag aaagatggcc ggcggcgccg gcacacccga
     1081 ccaaattcct gcaaatgcca ccgccagcaa acaccccatt gcgtacatgg aatcactccc
     1141 gatccgcgat ccgtcagtga gttaaaagga catgccacaa cccgctggag agccagcatg
     1201 agaacgccgg cagtctgcct ggcagacgcc ttgtacgtag tctacctctt gaattgcatg
     1261 aaataaagca gaacggtcac gccgtcatgc gcggtatcgg aattctgtcg gggcgtctct
     1321 tcatcagctt tcccatggga taaccggaga gaccaggatg aaatctgtgc caatggagca
     1381 gacctcgacg tctgccgggc ccgcggcagc tcattcgggc cgtcgcagca acggaagtaa
     1441 acaaaagcag cgggtgcaac acgtacaggt tgcaacccgc tgcatttcta cagtggcgct
     1501 caagatggca gaccggccta agctcaggtg ccgggaaaac gcgcaacccg gcggcaaacc
     1561 cccagtctcg ggtgaggcct tggcgttccg ggctcacagg cgagcctgtc ggggctgagg
     1621 catgcatttc agcctgccac ggtgattcgc cgcatactgg caggcagcca actcttcagg
     1681 cggaggaaaa gcgcccgtca cccggtctct tatttgacgg gggttttcat atcctcaaga
     1741 atggcttcca ggtcttcctc gtgctccacc tcgtctttga gaatgtcaaa gatcagatgg
     1801 taggtgatgt cgtcttttcc ccggacgaaa tcgatgagtt tcttgtacgt ctcaatcgcg
     1861 cactgctcgc ccttgatatt ctgctcgagc aggacaactg tattgggatt ggccggctct
     1921 tcgtagccgc aattggtttt tttcatccac tccgccgggt tcaacagagg ggtgccgccc
     1981 agctgctgga ttcgttccgc aagcttctcc gca
//
LOCUS       DAOPBQ010000601         2008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_44160_length_2008_cov_4.022017, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000601 DAOPBQ010000000
VERSION     DAOPBQ010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44160_length_2008_cov_4.022017"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKW60_14620"
     CDS             complement(<1..310)
                     /locus_tag="PKW60_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00717.1"
                     /translation="MRDTKTQFLGLLCFLLFLGLLLTNGFVNRIFAQQSDIDVFTQIE
                     PIGNVLDEILKSYVYEPDLNEVVEGGLKGMMNALDEHSSYIPPDVYKELTDETQGEFD
                     G"
     gene            complement(607..1461)
                     /locus_tag="PKW60_14625"
     CDS             complement(607..1461)
                     /locus_tag="PKW60_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HPK00718.1"
                     /translation="MRNRGFTLIELLVVIAIIGILAAILLPALARARESARRASCANN
                     LKQMGLIFKMYANESKGEKFPHMMIFDCAGVQLPFEQAVALEEVYPEYLTDLNVLICP
                     SAANGGTPLELWDEGRTVGHYAPYGGAPDGVVQPCEVTGHPYSYNGWALTYSLVSTDY
                     ALFHDNLTDFVENALEADPARVDQDWKLFAAMEGQDTLYRLREGIERFMVTDINNPSG
                     SAQAQSAIAVMWDQVAEDASHFNHIPGGSNVLYMDGHVEFVRYPGEFPCDQGGLMLHE
                     CSHHEHTP"
BASE COUNT          496 a          562 c          547 g          403 t
ORIGIN      
        1 taccgtcaaa ctcgccctgg gtctcgtccg tgagttcctt gtagacatcg ggcggaatat
       61 acgagctgtg ttcatccagc gcgttcatca tgcccttgag cccgccttcc accacctcgt
      121 tcaggtcggg ttcatagaca tagctcttca ggatttcgtc aaggacgttt ccaatgggtt
      181 cgatctgggt aaacacatca atgtcggatt gctgcgcgaa gatgcggttc acgaagccgt
      241 tggtcagcaa caatccgaga aagagcaaaa agcacagcag acccaggaat tgcgtcttcg
      301 tatcgcgcat gtagtatctc cagtcggcaa acatccagac ttcgcacggg tgaaaaagac
      361 ctcgggcgcc cgcagtaggc cccgacgccc acatgaatta tgataccaga cgcccgtgtg
      421 tattgcgaac cggccaggtt gattggagca gcgcaacacg cctcggtaaa gcatcgattt
      481 tcaatgcctt gccggaaaac ggcaaccggc ggaacctgtg ttgcctggtc aaaaggcccg
      541 aaaacaacat gtgggcggcc cccatacgcc gcccgcatgt ttttcagcca gccgctcgct
      601 caggattcag ggagtatgct catgatggct gcactcatga agcatgagtc caccctggtc
      661 gcaggggaat tctcccggat aacgtacaaa ctcaacatgc ccatccatgt aaaggacgtt
      721 gctgccgccg gggatatgat tgaagtggct ggcatcttcg gcaacctgat cccacatgac
      781 cgcgatcgcc gattgcgcct gggcgctgcc ggacggattg ttgatgtcgg tcaccatgaa
      841 ccgctcgatg ccctcgcgaa gccggtagag cgtgtcctgc ccctccatgg cggcgaacaa
      901 cttccaatcc tggtcaacgc gggcgggatc cgcctccaga gcgttctcca caaaatccgt
      961 caggttgtca tggaacagtg cgtaatctgt gctgacgaga ctgtaggtaa gcgcccagcc
     1021 gttgtaggaa tagggatgac cggtaacttc acagggttgc accacgccat ccggggcgcc
     1081 gccatagggt gcataatggc caaccgtgcg tccttcgtcc cagagctcca ggggagttcc
     1141 gccgttggcc gcactgggac aaatcaatac attgaggtcc gtcagatatt ccgggtagac
     1201 ctcttccagg gccacggcct gttcaaacgg caattgcaca cccgcgcaat caaagatcat
     1261 catgtgcggg aatttctctc ctttcgattc gttggcgtac attttgaaaa tcaaccccat
     1321 ctgtttgagg ttgttggcgc aactggcacg acgggcggat tcccgggcgc gagccagggc
     1381 cggcagtaga atcgcggcaa gaatgccgat gattgcgatc accacgagca gttcgatcaa
     1441 tgtaaatcct ctgtttcgca tgagtgcaca cctcgtatgt cggtttcagg tatgaaattc
     1501 ggcttcagac agaaatacgg cgagagacct tccggctcgc gccccagagc gcccggaaag
     1561 acgggaggcc gtacggatgc agctacgcag taagtggagg cccacgcagg aaaaagaagc
     1621 tgcagtacgg aaaacggaaa aggacccgga gcagcattac agcctgggca atgcgccgca
     1681 ggaataaaaa aaccgtccgc atcgcgcagg cgagcccggg cgtcagaaag agggcacaga
     1741 aggcgcaaca cgggtcttgt tcatgatgag cgtgaagaaa aggaacgaaa aaatgcacga
     1801 gcgcaacggc ataaagcgcc accagcagaa cggcaatgcc attgcgaaaa aacctaccgc
     1861 tctttttacc aatgagtgcc ataggaacag catcaacagg gttatgaaaa ccgtataccc
     1921 caattttatc ttaatttatc ctattgtcaa tacctcgaat cgcgccatct aagatcttac
     1981 cggagatggt atcgtcctgc catttgcg
//
LOCUS       DAOPBQ010000602         1976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_45121_length_1976_cov_3.075482, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000602 DAOPBQ010000000
VERSION     DAOPBQ010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45121_length_1976_cov_3.075482"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1947)
                     /locus_tag="PKW60_14630"
     CDS             complement(<1..1947)
                     /locus_tag="PKW60_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00719.1"
                     /translation="MLMGQPAPQPSAPICGFALLAAETGPQLATFGGNACGVCLREGE
                     EAELAVPLFNVPEGATVQASIRVEGTGGVRLAVSAGSSTATYEYAGEASAAIVINPEM
                     AQESRLALQLTGTAGEILVRFSDIRLVYGDRQLAVRLTPEEALEPIAPPPALPALRPI
                     LQAALLEWDWRLQDGIGTSREPVSYALAIERLFERGDALIADLASTGLPIDTARWQEL
                     KGEFTSLDGVSDEDPRWEALWLGVHTARRSIVFSNPLADVGPLLFVKHVPSYFSHQLT
                     QYYGRCARGGGGVYVLDAPGMSMECRELVSSQLPLGNYMHPEVTHDADRILFAFCEVP
                     FEPSDEVEEQCYERWYHLFEVRPDGTGLRQITEGPHDDFSPRELPDGNLMFISTRRGG
                     YHRCGRGPCDVYTLALADGDGANPRTVSYHETQEWDPAVLADGRVIYTRWDYVDRNAV
                     HYQHLWSVRPDGTGPAIYYGNNTLNPVGIWEARQVPASTQVMATAAAHHAMTAGSIIL
                     LDTTRGVDGLEPVTRLTPDAPFPESETHVLPGGWHAPGSPKEYDTPEEARRWPGHCYR
                     SPYPLSETYFLAAYSFDPLIGEPAGNKPNMFGLYFVDAFGNKELLYRDLNISSLWPVP
                     IRPQARPPVIPSTCDPKLAEEDEGT"
BASE COUNT          360 a          639 c          627 g          350 t
ORIGIN      
        1 cgtgccttcg tcttcctcgg ccagtttcgg atcgcacgtc gacggaatca ccggaggccg
       61 ggcctgcggg cggatgggca ccggccacag gctcgaaatg ttgaggtcgc ggtacagcag
      121 ctccttgttc ccgaaagcat ccacgaaata caggccgaac atattgggtt tgttcccggc
      181 cggctcgcca atcaacgggt cgaaactata ggcggccaga aaatacgtct cgctgagagg
      241 ataggggctg cggtaacaat ggcccggcca gcggcgggcc tcctcgggcg tgtcgtactc
      301 tttcgggctg ccgggcgcat gccatcctcc cgggagcacg tgcgtctcac tttcagggaa
      361 cggcgcgtca ggcgtgagcc gcgtcacggg ttcgagcccg tccaccccgc gggtggtgtc
      421 gagcagaatg attgatccgg cggtcatggc gtggtgagcg gcggctgtcg ccatgacctg
      481 agtcgaggcg ggcacttggc gcgcttccca gatccccacc gggttgagcg tattgttccc
      541 gtagtagatc gccggcccgg tgccgtctgg gcgcaccgac cagaggtgct ggtaatgcac
      601 ggcgttgcga tcgacatagt cccatcgtgt gtagatcacg cggccatcgg ccagcaccgc
      661 gggatcccac tcctgggttt catggtacga aaccgtgcgc ggattcgccc cgtcaccgtc
      721 cgccagggcg agggtgtaca catcgcaggg cccccggccg cagcgatggt agcccccgcg
      781 ccgcgtcgag atgaacatga gattgccgtc gggcagttcg cgcggggaaa aatcgtcgtg
      841 aggaccttcc gtgatctgcc gcaggccggt cccatcgggg cgtacttcga agagatgata
      901 ccagcgttca tagcactgct cttcgacctc gtcgctgggc tcgaacggca cttcgcagaa
      961 cgcgaacaaa atgcggtcgg catcatgcgt gacttcggga tgcatataat ttcccagcgg
     1021 caactggctc gacaccagtt cgcggcattc catcgacatg cccggagcat cgagcacgta
     1081 gacgccgccc ccaccgcgcg cacagcggcc gtaatactgc gtcagctggt gactgaaata
     1141 cgagggaacg tgtttgacga acagcaacgg tccgacatcg gccagggggt tggaaaagac
     1201 gatgctgcgg cgcgcggtat gcaccccaag ccacagcgcc tcccatcgag gatcctcgtc
     1261 agagacgcca tccagtgatg tgaattcgcc tttgagttcc tgccaccggg ccgtatcaat
     1321 cggcaagcct gtgctcgcaa ggtcggcgat caacgcgtcg ccccgttcga acaggcgctc
     1381 aatggccagc gcgtacgaga cgggctcgcg cgatgttccg attccgtcct gcaggcgcca
     1441 atcccattcc agaagcgcag cttgcaatat tggtcgaagc gcaggaagcg ccggtggcgg
     1501 cgcaatcggc tccagggcct cttcgggcgt caggcgcacg gcaagctgtc tatcgccata
     1561 gacaagccgg atgtccgaga accgcaccag gatctcgcca gccgtcccgg tgagctgcag
     1621 cgccaagcgc gattcctgcg ccatttccgg gttaatcacg atggccgccg aggcctcgcc
     1681 ggcgtattcg tacgtggcag tgctgcttcc cgcgctcaca gcgaggcgca ctcctcctgt
     1741 gccttccacc ctgatcgatg cctgcacggt cgcgccttcc gggacattga acagaggaac
     1801 ggccagctcg gcctcttctc cctcacgcag gcatacgcca caggcattgc ccccaaatgt
     1861 tgcgagttgc gggccggttt ccgcggcgag cagcgcaaaa ccacagatcg gcgcactggg
     1921 ctgcggggcg ggttgaccca tcagcataac cgatggctcg ccggcgcgcg gccggt
//
LOCUS       DAOPBQ010000603         1973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_45227_length_1973_cov_3.803441, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000603 DAOPBQ010000000
VERSION     DAOPBQ010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45227_length_1973_cov_3.803441"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="PKW60_14635"
     CDS             <1..1144
                     /locus_tag="PKW60_14635"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPK00720.1"
                     /translation="RLLTDFTYKRTMAQLESDVRQARMALERTERKAAADVVQSEADL
                     KARESEYLRQKSKLEKIIAQIDKTKIYAPADGLVVYATSVRGSWRGSTEPLEEGQVVR
                     ERQELIYLPTGSSFMAEVDVHEASLTKIKPGLPVRITTEAAPDKTYLGRVSSVAPLPD
                     AQSVWLNPDLKVYNTDIIIEGESNGLRTGVTCLAEIIVERYDNALYIPVQAVVRVGGK
                     PTVYVAKSTGVSPRQVELGLDNNRMIHILNGLEQGESVLLNPPLADDKNENGESPEDD
                     FLAISAEPAASPEAAPAPEAAPAPEAARAPEREREQRSEGAEATNADGARERFRNASP
                     EEREKMRKEMEERLQKMSPEEREQFMQQRRGRRGGGRSSEGAPGNP"
     gene            1147..1857
                     /locus_tag="PKW60_14640"
     CDS             1147..1857
                     /locus_tag="PKW60_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK00721.1"
                     /translation="MNTAQSDSSTDVIRLEHVSKVYTMGEQEVRAVDNVSLAFARGAF
                     CAVMGPSGSGKSTMLNLLGCLDRPSAGRYMIAGEDVSKLDDDRLSDIRLKHLGFVFQS
                     FNLIPQLTVLENIALPLFYLGWSTEKSAAHAAELAKIVGLAARLSHRPTELSGGQQQR
                     VAIARALANDPKIILADEPTGNLDTSTGDQIMALLRDLNQQGKTIIMVTHEPSIALHA
                     KRRIHMLDGRIERIEDDA"
BASE COUNT          440 a          609 c          591 g          333 t
ORIGIN      
        1 acgcctcctc accgatttca cctacaaacg gacgatggcg cagctggaga gcgatgtccg
       61 ccaagcgcgc atggcgttgg agcgcacgga acggaaagcc gcggccgatg tcgtgcaatc
      121 cgaagccgac ctgaaggcca gagaatctga atacctccgc cagaaaagca aactggaaaa
      181 aatcatcgct caaattgata agacaaaaat ctatgccccc gccgacgggc tggtcgtgta
      241 tgccacatcg gtccggggca gctggcgcgg cagcactgaa ccgttggaag aggggcaggt
      301 agtgcgtgag cggcaggaac tcatctatct gccaaccggg tcgtcgttca tggccgaggt
      361 cgacgtccac gaggccagtc tcaccaagat caagccggga ctgcccgtcc ggatcaccac
      421 tgaagccgcg cccgataaaa cgtacctggg ccgggtttct tcggttgcac cgctccccga
      481 tgcgcagagt gtctggctca acccggatct gaaggtctac aacaccgaca tcatcatcga
      541 aggagagtcg aacggcctcc gcaccggcgt gacatgcctg gccgagatca tcgtcgagcg
      601 ttacgacaac gcgctctata ttcctgtgca ggctgtcgtc cgggtgggag gcaagcccac
      661 cgtgtatgtc gcgaaaagca ccggcgtttc accgcgacag gtcgaacttg ggctcgacaa
      721 taaccgcatg attcacatct tgaacgggtt ggaacaggga gaatccgtct tgctgaatcc
      781 gccacttgct gacgacaaga acgaaaacgg cgaaagtccg gaggatgatt tcctcgcgat
      841 tagcgcagaa cccgctgctt cgccggaagc tgcgcccgcg ccggaggctg cgcccgcacc
      901 ggaggccgct cgcgccccgg aacgagagcg cgaacagaga tcagaagggg cggaagccac
      961 caatgccgac ggcgcgcgtg aacgcttccg caatgcctcc ccggaagaac gcgagaagat
     1021 gcgcaaggaa atggaggagc gcttgcagaa aatgtcacct gaggaacggg agcagtttat
     1081 gcagcaacgc agaggccggc gaggcggcgg acggtcgtcc gagggagcac ccgggaatcc
     1141 gtgacagtga ataccgccca gtctgattcc agcacggatg tcatccgtct ggagcacgtc
     1201 tccaaagtct acacgatggg ggagcaggaa gtgcgtgccg tggacaacgt gtccctcgcg
     1261 tttgcccgcg gggccttctg tgccgtgatg gggccgagtg gttcgggcaa gagcaccatg
     1321 ctgaacctcc tcggatgcct cgaccgcccc agtgccggcc gctacatgat cgccggcgag
     1381 gatgtgagca aactcgacga cgaccgtctg agcgatatcc gtctcaagca cctcggattc
     1441 gtgttccaga gcttcaatct cattcctcag ctgactgtgc tcgagaatat tgcccttccg
     1501 ctgttttacc tgggatggag cacggagaag agtgccgcgc acgccgctga actggccaaa
     1561 attgtcggcc tggccgcgcg cctcagccac cggcccaccg agctgtccgg cggacagcag
     1621 cagcgggtcg ccatcgcgcg cgcgctcgct aatgatccca agatcatcct cgcggatgag
     1681 ccgaccggca acctcgacac ctctaccggc gaccagatca tggcgctcct ccgcgacctg
     1741 aaccagcagg gcaagaccat catcatggtc acgcatgagc ccagcatcgc gcttcatgca
     1801 aaacggcgaa tccacatgct cgacggccgc atcgaacgca tcgaggacga cgcctgatgc
     1861 agattttcaa tgtcgtgcgg aacatacacc tgggcatcaa atcgcttatg ctgcacaagc
     1921 ttcggtccct gttgaccatg ctcggcattg tgttcggggt ctcgagcgta att
//
LOCUS       DAOPBQ010000604         1968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_45394_length_1968_cov_3.892838, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000604 DAOPBQ010000000
VERSION     DAOPBQ010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45394_length_1968_cov_3.892838"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKW60_14645"
     CDS             complement(<1..376)
                     /locus_tag="PKW60_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HPK00722.1"
                     /translation="MSGYFVHPTSIIDEACEIGEGTRIWHFSHVLRNSRIGKGCNIGQ
                     NVMIGPDVTIGSNCKIQNNVSVYKGVRLENDVFCGPSVVFTNVINPRSHINRRDEFRE
                     TLIERGASLGANATVLCGVRIGQ"
     gene            complement(400..1389)
                     /locus_tag="PKW60_14650"
     CDS             complement(400..1389)
                     /locus_tag="PKW60_14650"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00723.1"
                     /translation="MANLCVVGAGYWGKNLVRTFHELGVLYAVCDVNPATLRAFEEPH
                     PDAQLIPDPRHVLEDSRIDAVAIAAPAELHFALAKQALEAGKHVFCEKPLALRASEGR
                     MLCELADSAGRVLQVGHLLEFHPAVIAMASLVAQGELGELWYAWSNRLKFGRFRTEEN
                     VLWSFAPHDISILNAVLGRAPVQVSAASQTCVTQGVADVAVVRLEFAGALRAHIHVSW
                     LNPFPERKVVVVGSRRMAVFDDLAEGTQLQVFETGQRREDGVPVALPVRGKPIETPGE
                     PPLLAECRHFLDCIERGVRPRTDGESALKVLRVLEACQESMDTGGQTVEVASS"
     gene            complement(1478..>1968)
                     /locus_tag="PKW60_14655"
     CDS             complement(1478..>1968)
                     /locus_tag="PKW60_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00724.1"
                     /translation="FCRLAFEVSQEVPEVEFVLAGFATGDGCSTEYHDLVHGMGTQPL
                     DAEEFARRAASLTYSVWAGDPEYYRLVASASFLDSLYYGKPGIYLRNPYVEHYFERMG
                     DIGYLVDSYEEMHELLIQLARDFPEERYRRQCATIVQARKALSPEALSGVFRSIVDRA
                     RA"
BASE COUNT          408 a          640 c          527 g          393 t
ORIGIN      
        1 actggcctat acggacgccg cacaagactg tcgcattggc gccgagcgag gccccgcgtt
       61 cgatcagcgt ttccctgaac tcatcgcgac ggttgatgtg gctccgggga ttgatcacgt
      121 tggtgaagac caccgaaggt ccgcagaaca cgtcattttc gagccgaacc cctttgtata
      181 cgctcacgtt attctggatc ttgcagttgc tgccaatggt cacatccggg ccgatcatga
      241 cgttttgtcc gatattgcag ccttttccga tgcggctgtt gcgaagcaca tggctgaaat
      301 gccatatccg ggtgccttcg cctatttcac aggcttcgtc aatgatcgat gttggatgca
      361 caaaatatcc gctcatggta ctccttttgt ttcatcattc tatgacgacg ccacctcgac
      421 ggtttgaccg cccgtgtcca tagattcctg gcacgcctcc agtacgcgga gcaccttcag
      481 cgcgctctcc ccatccgtgc gtgggcggac gccccgctcg atgcagtcca gaaaatgacg
      541 gcattcagcc agaagcggtg gttcacccgg cgtttcaatg ggcttgcctc ggaccggcag
      601 cgccaccggc acgccatcct cccggcgctg gccagtttca aacacctgca actgggtccc
      661 ctcagccagg tcgtcgaaaa cggccatccg cctgcttcca accacgacta ccttccgttc
      721 ggggaatgga ttcagccagc tcacgtggat gtgagcgcgc aatgcgcccg cgaactcgag
      781 ccggaccact gccacatcgg caacaccttg cgttacgcag gtttgagagg ctgcgctcac
      841 ctgcacgggc gcacggccca gcactgcgtt caagatcgag atgtcgtgcg gagcaaaact
      901 ccacaagaca ttctcttcgg tccggaacct gccgaatttc aagcggttcg accaggcgta
      961 ccagagctcg cccagctcgc cttgcgccac cagcgacgcc atagctatca ccgccgggtg
     1021 aaattcgagc aaatgcccca cctggagcac ccggccagcc gaatcggcca gttcgcaaag
     1081 catgcggcct tcggaggccc ggagcgcgag cggtttctcg caaaaaacgt gttttcccgc
     1141 ctcaagcgcc tgctttgcca gcgcaaagtg cagctcggcg ggggcagcga tggccaccgc
     1201 gtcgatccgg ctgtcctcaa gcacgtgccg ggggtccgga atcagctggg catcgggatg
     1261 cggttcttcg aatgccctca gggtcgctgg attgacatcg cagaccgcat aaagaacccc
     1321 cagttcgtgg aaagtccgaa cgaggttttt cccccagtat ccagctccca caacgcagag
     1381 attggccact gctctgtccc cccgtgacgt tgcctgaacg gatattatgg tactggtttc
     1441 gctgaagcgc ccgcaaatgc ctgccagaga gccctgatca tgccctggcg cgatctacaa
     1501 tcgatcgaaa gacgccactc aaggcctcgg gggacagagc ctttcgcgcc tgaacgatgg
     1561 tggcgcattg ccgccggtag cgctcctccg ggaaatcacg cgccagctgg ataagcaatt
     1621 cgtgcatttc ttcgtaggaa tccaccagat atccgatgtc tcccatccgc tcaaaatagt
     1681 gctcaacgta gggattccgc aggtaaatgc cgggtttacc gtaatacagt gaatccagaa
     1741 acgacgcact ggccaccaac cggtaatact ccgggtcgcc cgcccacacg gaataggtaa
     1801 gcgaagcggc tcgcctcgca aactcctccg catctaaagg ctgtgtgccc atgccatgca
     1861 ccagatcatg atactcggtg ctgcatccat ctcctgtcgc aaagccggcc aggacgaact
     1921 ccacctccgg tacttcctgc gacacctcga atgccaggcg gcagaacc
//
LOCUS       DAOPBQ010000605         1959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_45693_length_1959_cov_2.414391, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000605 DAOPBQ010000000
VERSION     DAOPBQ010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45693_length_1959_cov_2.414391"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            561..>1959
                     /locus_tag="PKW60_14660"
     CDS             561..>1959
                     /locus_tag="PKW60_14660"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HPK00725.1"
                     /translation="MYRTRIPLGIRIVVAALLLCAFAPAYSQSAGLVNDRGWFVQGDT
                     VIWGWVQHNGWWREGQRANITRRSVGDPLGDVRPNRTEDLDALTDSMLRYGYPAFEHN
                     YGLWYDRRRDAHDAAPRTDPNVVPPFLEQPWARSDSGTAFDGLHQYDLTAFNPWYFER
                     LREFAGLCDRKGAVLIHKFYMQHALLETPAHYVDFPWRPGNCIQDTGLPDAIPAANVF
                     YDVSHPVRRELHRLYIRHCLDVLGDFRNVIHLTSQEFTGPTDFVLFWLDEIRAWEKET
                     GKHVCIGLGAPKDVQDAVLARPEQAKSIDVLDLRYWWINADGNLVAVQGGQEIPGRGL
                     ESGAWQAEQSPPERIYAKIRAIRDQYPGAGILDAIEATRQQSWAFLMGGGGLLVRGQI
                     AYPDFADPSEYIQPAGVDVILPTYHWIRTELATQLPGMRPADVVASSASPTWCLAGPA
                     GPWLVYALEGGRFSLN"
BASE COUNT          383 a          594 c          575 g          407 t
ORIGIN      
        1 cgacgcgccg ccattgatat atcgtgacac cgtctgccgg ctgacgttgg cccgctcggc
       61 cacgtcaatt accgtcggca cacgccggtg tcgtctcatg aattaacctc tgcctgctga
      121 cccgatgagc ccaagccacc ccccgctcgg tcctcatcaa aatccactga accattactg
      181 gccctatttt accagagtga gtgatcgttc acaacttccg aatcgcgcca tctaagcgtc
      241 tgcccgtaat ggtattggcg tgtcattcga gggcttgctg aaagatggga gggaggggcg
      301 gtgtcaggcg cgtgaatgtt gggtggttag aacctgtatc gggaaataat gatgggattc
      361 tattggacga gcgcatcctg aacagccatc agcagcacct tgttggggtc gtaaaaggcg
      421 tcttcgcctt caaccttcaa attgatacag gccgatttct gtgcctatag taccgaaaga
      481 acgaatcttc gagaatgaac tgcatcagcc ctggtgaagg ttagcggtgc agacaggcgc
      541 caggaaagga cccgcgaccg atgtacagaa cccgcatccc gcttggaatc cgtattgtcg
      601 tggccgctct tctgctctgt gcgttcgcgc cggcgtacag ccagagtgcg gggttggtga
      661 atgatcgagg ctggttcgtt cagggcgata ccgtcatttg gggctgggtg caacacaacg
      721 gatggtggcg agaggggcag cgggccaaca tcacacgccg ctcggtcggc gatccgttgg
      781 gcgatgtgcg gcccaatcgc accgaggacc tggatgcgct gaccgacagc atgttgcgct
      841 acggctatcc cgcattcgaa cacaactatg gactctggta tgaccggcgc cgcgatgcac
      901 acgacgcagc tccccgaact gatccgaacg tcgtcccgcc gtttttggag cagccctggg
      961 cccgcagcga ctccggtact gcgttcgacg gcttgcacca atatgacctt accgccttca
     1021 atccgtggta tttcgagcga ttacgtgaat ttgccgggct ttgcgaccgc aaaggcgccg
     1081 tgctgattca caaattctac atgcagcacg cgctgcttga gaccccggct cattatgtcg
     1141 attttccctg gcgtccgggc aactgcatac aggacaccgg cctgcccgac gcgatccccg
     1201 cggccaacgt cttttacgac gtcagccatc cggttcgccg ggagcttcac cgcctctata
     1261 tccgccattg cctggatgtc ctcggagact tccgcaacgt gatccacctc acgagccagg
     1321 aattcacggg ccccacggat tttgtgctgt tctggctcga tgaaatacgg gcctgggaga
     1381 aggaaacggg caaacatgtc tgtatcggcc ttggcgcacc caaagatgtg caggatgccg
     1441 ttctcgcccg ccccgaacag gcaaagagca tagatgtgct cgatttacgg tattggtgga
     1501 tcaacgccga cggaaacctt gttgcggtgc agggaggcca ggaaatcccg ggtcgcggcc
     1561 tggaatccgg cgcctggcaa gccgaacaga gccctccaga gcgcatttac gccaaaatcc
     1621 gcgccatccg ggatcagtat ccgggcgcag gtattctgga cgccatcgag gccacgcggc
     1681 agcaatcctg ggcgtttttg atgggaggcg gcggacttct cgttcgcggc cagattgcct
     1741 atcccgattt cgccgatccg tctgaatata tccagcccgc gggagtcgat gtgatcctgc
     1801 cgacgtacca ttggatccgg accgagctgg cgacccagct tcccggcatg cgccccgcgg
     1861 acgtggtagc ctcttcggcc agcccaacct ggtgcctggc cgggcccgcc ggcccgtggc
     1921 tcgtgtatgc gctggaaggc ggccgttttt cgctcaaca
//
LOCUS       DAOPBQ010000606         1957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_45760_length_1957_cov_3.186120, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000606 DAOPBQ010000000
VERSION     DAOPBQ010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45760_length_1957_cov_3.186120"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(490..1212)
                     /locus_tag="PKW60_14665"
     CDS             complement(490..1212)
                     /locus_tag="PKW60_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00726.1"
                     /translation="MNGHEENVATVPLQPFQCISSGWDLIKGEYCLFLGITFVGLLLG
                     SLVPFGIIAGALYCGIFMALLMKMRGLPVEFGVLFKGFDYFLESLLVTLAIFAVGAVF
                     AVLMVIALAVLFAVGGGIGKSANSGELASLFILPGGFVLLLIAFVFSIFLYGWSFLAY
                     PLLVDRNMQAWPALKLSARAVWANKWGVAGLMLLNGIFGMVGAVLCYVGLFVYLPVMF
                     ASLAVAYRKLFPELAPPDAPEM"
     gene            complement(1209..>1957)
                     /locus_tag="PKW60_14670"
     CDS             complement(1209..>1957)
                     /locus_tag="PKW60_14670"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK00727.1"
                     /translation="DLRAQCAAVSDYAICLGGAGIPDIVNGVGRGRGMERVLLDIMMQ
                     DEVGVAVIDRRCAFYYEYCRRALETAGDLIDILCLGEDCGNQLGPMFDPEVFDRFFRP
                     RLQQFYDLGYEFGCKVMMHSCGSTRKLIPRFIEMGLEVLDAVQPEPAGMNPGELKHEF
                     GDKLAFCGMISTQHTLPHGTEADCRAEARHRIDVIGKGGGYIFSPAHCIQPDTPLVNV
                     LAIYEEALGLPEGALLDARPAAGTGKEQDP"
BASE COUNT          446 a          625 c          552 g          334 t
ORIGIN      
        1 cccccagcgt ttccgtttgt tacgggtcag gaacgttcgc gaacgtgttc cattcaagat
       61 aacgattctg tgtccatttc gttcaagtaa tcggcatcgc gctgaaaaaa gccgaatatt
      121 ccacccccgg ctcaagtgtc cccgtccata ccgtccataa cgtccaccgg aagacagaaa
      181 tgcaggcgag atgccggggc ttcaagacaa gaatgcccgc cgggggcgcc gtattgcagc
      241 cccgcagacg gtgaacatgc gactccgggg accccggaac ggggtccaat ttgaatagcc
      301 gtgggcgcag cccatggatc tgtccccgtc cataccgtcc attacgcgcc aggtgaggag
      361 ctgccgccgg ccattattct ccacccccac caatggcgcc tggccgcgcg cgatcacgcg
      421 gggctggaat cccccccggc catgcggtgc tcacgcggtt tgcggacaca gggcctgact
      481 gccgcgtctt cacatctccg gagcgtccgg gggcgccagt tcgggaaaca gcttgcggta
      541 agccacggcg agggaggcaa acatcacggg caggtacaca aacaggccca cataacacaa
      601 gacagccccc accatgccga agatgccatt gaggagcatg aggcccgcaa cgccccattt
      661 gtttgcccac acggcccgcg ccgacaactt gagcgccggc caggcctgca tattgcggtc
      721 cacaagcagc ggataggcaa ggaacgacca gccgtacaga aatatactga acacgaacgc
      781 gatcaggagg agaacaaacc ctccgggcaa aataaaaagc gaagccagtt ccccggaatt
      841 ggcgcttttt ccgataccgc cgcctacagc aaagagcacg gccagggcga ttaccatcag
      901 caccgcaaac acagccccga cggcaaaaat cgcaagcgtc acaagcaggc tctcgagaaa
      961 atagtcgaag cctttgaaca agacgccgaa ttctacgggc aatcctcgca ttttcatcag
     1021 cagcgccata aaaatgccgc aatagagcgc tcccgcaatg atcccgaaag ggaccaggct
     1081 tccgaggaga aggccgacga atgtaatccc cagaaagaga cagtattccc ctttgatgag
     1141 gtcccatccg cttgaaatac actggaaagg ttgcaggggg acggtcgcca cattttcttc
     1201 atgtccgttc atgggtcctg ctccttccct gtgccggcgg cggggcgcgc gtcgagcagc
     1261 gcaccttcgg gcaggcccag ggcttcttcg tagatggcca gaacgttcac caaaggcgta
     1321 tccggctgga tgcaatgcgc cgggctgaag atgtagcctc caccctttcc aataacgtca
     1381 atgcggtggc gtgcctccgc gcggcagtcg gcttcggtgc cgtgcggcag ggtgtgctgg
     1441 gtgctgatca tgccgcagaa tgccagcttg tcgccgaatt catgtttcag ctccccgggg
     1501 ttcatgccgg cgggctcggg ttgtaccgcg tccagcactt ccagccccat ttcaatgaac
     1561 cggggtataa gcttgcgcgt gctgccgcag gagtgcatca tcaccttgca gccgaattca
     1621 taaccgagat cgtaaaactg ctgcaaacgc ggccggaaaa agcggtcaaa cacctccgga
     1681 tcaaacatgg gtcccaactg gttgccgcag tcttcgccga ggcagaggat gtcgatgagg
     1741 tcgccggcgg tctcgagtgc gcggcggcag tactcgtaat aaaaagcgca gcgccggtca
     1801 atgaccgcaa cccccacctc gtcctgcatc atgatatcca gcagcacgcg ctccatgccg
     1861 cggccccggc ccacaccgtt cacgatatcc ggaatcccgg cgccacccag gcagatggcg
     1921 taatccgaca ccgcggcgca ttgggcgcgc agatccg
//
LOCUS       DAOPBQ010000607         1950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_45997_length_1950_cov_3.366227, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000607 DAOPBQ010000000
VERSION     DAOPBQ010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45997_length_1950_cov_3.366227"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PKW60_14675"
     CDS             <1..709
                     /locus_tag="PKW60_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPK00728.1"
                     /translation="NLFPSAAITASTMPGISSGLGMQAIERVAAETLPEDYGVAWSGM
                     SYQEKQTGSEGLVLVALALVFGYLFLVAQYESWTVPVPVILSLPTAMLGAVLGLKFTG
                     MSLSIYAQLGLIMLVGIASKNAILIVEFARDSRESGGKSILDAGTNAMYERFRPVLMT
                     AFTTFLGTLPMVLATGAGSGARRSIGTTLFAGMTLATVLGILFIPALYVMFQATRERL
                     KRVLHISPTTQQQEEIS"
     gene            706..>1950
                     /locus_tag="PKW60_14680"
     CDS             706..>1950
                     /locus_tag="PKW60_14680"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK00729.1"
                     /translation="MMRRILQRIACALTLVYTGCATVGPDYVAPEIPEAPVEDVLQKG
                     DGEATAESGPVTAELLADWWQTLEDPLLTELIRDSLEGNLNIRQAQSRVRMARQIAGI
                     AEAGLYPHVDGAAAYQRSRMSQNRVEGPSQTDKVQGAARNVAAAMGAAQNWSLMMQDP
                     SRGILTVPGQIASWPFPREVDWESDFYRAGVDAAWEIDLFGGTRRAIEAAEADVDAAH
                     ENLNAAWVSLAGDVARSYIALRTYQARLAVAESNLRAQEETYTLLDSLYQSGLRDALA
                     VQQARYIMENTRALIPPLHSGLEAAMNSLAVLTGVMPGTLHERLNTTEPIPVASLKVV
                     TGIPANTLRQRPDVRMAERALAAQTARVGQAEAELYPKFFLAGSIGLESISSGTLLNS
                     DSRAWNVGPRVSWPIFHAGAIRR"
BASE COUNT          332 a          592 c          669 g          357 t
ORIGIN      
        1 taatctgttc cccagcgcag ccatcaccgc gtcgacgatg cctggcatca gttccgggct
       61 ggggatgcaa gccatcgagc gcgttgccgc cgagacgctt cccgaagact acggcgtggc
      121 gtggtcgggc atgagctacc aggaaaagca gacgggcagc gaagggctgg tgctggtggc
      181 gctggcgctc gtgttcggct acctgttcct ggtggcgcaa tacgagagct ggacggtgcc
      241 ggtgccggtg atcctgtcgt tgccgacggc catgctgggc gccgtgctgg gcctcaaatt
      301 caccggcatg tctctgagca tctacgccca gctggggttg atcatgctgg tgggcatcgc
      361 gagcaagaac gccattctta tcgtggaatt cgcgcgcgac agccgtgaga gcggcggaaa
      421 gagcattctg gacgcgggta cgaacgcgat gtatgagcgg tttcgtcccg tgctgatgac
      481 ggcctttacg acgtttctgg gcacgcttcc gatggtgctg gccacgggcg cgggttccgg
      541 cgcccggcgt tcgatcggca ccaccctgtt tgccggcatg acgctggcca ccgtcctggg
      601 tattttgttt attccggctc tgtatgtgat gttccaggcc acgcgtgaac ggctgaaacg
      661 agtgctgcat atctcgccga ccacacagca gcaggaggag atttcatgat gcgccgcatc
      721 ctgcagcgta tagcttgtgc gctgaccctt gtgtataccg gatgtgccac ggtaggcccc
      781 gattatgttg cgcccgagat tcccgaggcc ccggtagaag atgtccttca gaagggtgat
      841 ggcgaggcca cggcggagtc ggggcccgtt accgcggagc tgcttgcaga ctggtggcag
      901 accctcgagg atcctctgtt gacggagctc atccgggact cgctcgaagg caacctgaac
      961 atccgccagg ctcaatcgcg cgtgcgtatg gcccggcaga tcgccgggat cgccgaggcc
     1021 ggcctgtatc cccacgtcga tggcgcggct gcgtatcagc gcagccggat gagccagaac
     1081 cgggtagaag ggccctcgca gaccgataaa gtgcagggcg cggcgagaaa tgtcgcggcg
     1141 gcgatgggcg ccgcgcagaa ctggtctctg atgatgcagg acccgtcccg gggcatcttg
     1201 acggtgccgg gtcagatcgc gtcgtggccg ttcccgcggg aagtggattg ggagagcgat
     1261 ttttaccggg ctggcgttga cgcggcctgg gagatcgatc ttttcggtgg aacccgccgg
     1321 gcgatcgaag ctgcggaagc cgacgtggat gcggcgcatg agaacctgaa cgcggcctgg
     1381 gtatcgcttg cgggcgacgt ggcgcgaagc tacatcgcgc tgcgcacgta tcaagcccgg
     1441 ctggcggtgg ccgaatcgaa cctgcgggcc caggaagaga cctatacact gctcgattcg
     1501 ctttatcagt cgggcctgcg cgatgcgctg gctgtgcagc aggcacgcta catcatggaa
     1561 aacacccgcg cgctgatccc gccgctgcac agcgggctcg aagcggccat gaacagcctg
     1621 gccgtgctga ccggcgtcat gccgggcacg ctgcatgagc ggctgaacac cacggagccg
     1681 atcccggtcg cctcccttaa ggttgtcacg gggataccgg ccaatacctt gcgccagcgg
     1741 cccgacgtgc gcatggccga gcgcgccctg gccgcacaga ctgcgcgggt gggccaggcc
     1801 gaggccgagc tctaccccaa attcttcctg gccggctcga tcggtctgga atcgatcagc
     1861 tcgggaaccc tgttgaacag cgacagcagg gcgtggaatg tcggcccgcg cgtttcctgg
     1921 ccgatcttcc acgcgggcgc cattcgccgg
//
LOCUS       DAOPBQ010000608         1949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_46020_length_1949_cov_4.159979, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000608 DAOPBQ010000000
VERSION     DAOPBQ010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46020_length_1949_cov_4.159979"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /gene="lpxB"
                     /locus_tag="PKW60_14685"
     CDS             complement(<1..972)
                     /gene="lpxB"
                     /locus_tag="PKW60_14685"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPK00730.1"
                     /translation="MKRLFFVAGESSGDQHGAHLIRALRDMDPEVQCEGLGGQEMAAA
                     GMALHFDLAGHAIMGFTEVLQSLKMIRRLFKDTLARFDASRPDALVLIDYPGFNLRLA
                     RAAHSRGIPVIYYISPQIWAWKRGRLRTIARLVRKMLVILPFEKELYEQAGVDCVYVG
                     HPLLDHIPTVQIDGTLRGGLIIGLLPGSRQQEIQRLLPVMLETARGIRTRYPQARFVT
                     PSVNPEREAQIRTLAGEFPLEVLVGKMYEVLDAARFCLVASGTATLETALFGVPLAVL
                     YKVSYLSYWIARSLVRVKHISLVNILSGKGIVPEFIQHEASPERVLPA"
     gene            complement(969..1922)
                     /locus_tag="PKW60_14690"
     CDS             complement(969..1922)
                     /locus_tag="PKW60_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00731.1"
                     /translation="MVSTRVGVIGVGHLGYHHARNYAALDGVDLAGVVDIDETNGARA
                     ALDFQVPHFRELDDLLAQGIDAASVVVPTTLHHGFTLRLLEAGVDVLVEKPIAQSVEK
                     AAEMVAAARGAGRILQVGHIERFNGAVRALFDAVRQPRFIECHRLSPFPSRGDDVSVV
                     LDLMIHDLEIVRAMDGTRPVSVDAVGVPVFSKYEDIANARIRFASGCVANLTSSRISL
                     DRMRKIRIFSPEMYVSTDYSAEEVLIYRKRPGDLAPGQSPMELISVDSLPVDHEEPLR
                     MELASFVQCVRARTRPVVDGEDALQALQLAQQVIDCIRTSP"
BASE COUNT          397 a          610 c          604 g          338 t
ORIGIN      
        1 cgcaggcagc acccgttcgg gcgaggcttc gtgctgaatg aactcgggca cgatgccttt
       61 ccctgaaagg atgttgacca gtgagatatg tttgacccgc accagcgagc gggcaatcca
      121 gtacgacaga tacgagacct tgtacaggac cgccaggggc acgccaaaga gcgcggtctc
      181 gagcgtggcc gtgccggagg cgacgaggca gaagcgggcc gcatcgagca cttcatacat
      241 ttttccgacg agaacctcca gcggaaactc gcctgccagg gtgcggatct gggcttcgcg
      301 ctccggattt acactgggag taacgaaccg cgcctgcgga taccgcgtgc gaattccgcg
      361 ggcggtttcc agcatgaccg gcagcaggcg ctggatctcc tgctgacggc tgccgggcaa
      421 aagcccgatg atcagcccgc cgcgcagggt tccgtcaatc tgcaccgtgg ggatgtggtc
      481 cagcagcgga tggcccacat acacgcagtc cacgccggcc tgctcataca actccttttc
      541 gaagggcaga atgacaagca tcttgcgcac gagccgggca atagtgcgaa ggcggccccg
      601 tttccaggcc cagatctgcg ggctgatgta ataaatgact gggatacccc ggctgtgggc
      661 cgcccgcgcg aggcggagat tgaagcccgg gtagtcaatc agcaccaggg catcagggcg
      721 cgacgcatca aaccgcgcga gcgtgtcctt gaacagccgc cgaatcattt tcagcgactg
      781 cagcacttcc gtgaagccca tgatcgcatg tccggccagg tcaaagtgga gcgccatgcc
      841 ggccgcggcc atctcctgtc cgccgagtcc ttcgcactgc acttccggat ccatatctcg
      901 cagcgcacga atcaggtggg caccgtgctg gtccccagac gattctccgg ctacaaaaaa
      961 gaggcgtttc acggcgatgt ccgaatgcag tcaataacct gctgggcgag ctggagtgcc
     1021 tgaagcgcat cttcgccatc cacgaccggc cgggtccggg cccgtacgca ctggacgaac
     1081 gaggccagct ccatgcgcag cggttcctcg tggtcgacgg ggagcgaatc caccgagatc
     1141 agctccatgg ggctttgtcc gggcgcgagg tctccgggcc gtttacggta gatcagcacc
     1201 tcttccgccg aataatcggt ggagacgtac atctccggag agaaaatgcg gatctttcgc
     1261 atacgatcca gcgagatgcg gctgctggtg agattggcga cgcacccgga tgcaaaccgg
     1321 atgcgggcat tggcgatgtc ttcgtatttc gaaaacaccg gcacgcccac ggcatcgacc
     1381 gataccggcc gggtgccgtc catggcgcgt acgatctcga gatcgtggat catcaggtca
     1441 agcaccacac tgacgtcatc tccccggctc ggaaatgggc tcagccggtg gcattcaatg
     1501 aagcgcggtt gacgtaccgc atcgaagagt gcgcgcaccg cgccgttaaa ccgctcgata
     1561 tgccccacct gcagaatccg gcccgcgccc ctggcggccg cgaccatctc agccgctttc
     1621 tccacgctct gggcgatggg tttctcgacc aggacgtcca caccggcttc cagcagacgc
     1681 agcgtgaacc cgtggtgcag ggtggtgggg actaccacgc tggcggcgtc gatgccctgc
     1741 gccagaaggt cgtccagctc acggaaatga ggaacctgaa agtcgagagc ggcccgggcg
     1801 ccgttcgttt catcgatgtc tacgacaccg gccaagtcaa cgccgtcgag tgcggcataa
     1861 ttccgtgcat gatggtatcc gaggtgaccg actcctataa cgccaacgcg agtggaaacc
     1921 atgtagatcc ttccgcaggg agcgcatgg
//
LOCUS       DAOPBQ010000609         1944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_46214_length_1944_cov_2.900476, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000609 DAOPBQ010000000
VERSION     DAOPBQ010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46214_length_1944_cov_2.900476"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PKW60_14695"
     CDS             <1..989
                     /locus_tag="PKW60_14695"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPK00732.1"
                     /translation="ELYAETTLPLPENPSNTFRTEDTAIVALRVREGDDASCLNLNRA
                     QSPRLLGVNPDELAALRAFLPEGTQDDLWPLLTIELHDGAVPALVGDSDTALWGLQKK
                     TGIENGDVLIYRDDDGNEVTVRLVGRLPMRVSVFQGSILISDAWFTRLFPSEAGFRAF
                     LVDTPPDRADAIAAALNKTFEREGMDAEPAPQRLLRFYAVESTYLAMFLVLGGLGMVL
                     GSAALGIVVLRNLFERRSEIAMLRAVGFDQQALIRLFLMEYLVLFGAGVAIGGVAAAM
                     AMLPAAASAGTSSPIGLQALIFASIALTGIVCIVFAVRAGIGRENFDALRTE"
     gene            complement(996..>1944)
                     /locus_tag="PKW60_14700"
     CDS             complement(996..>1944)
                     /locus_tag="PKW60_14700"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPK00733.1"
                     /translation="FRQKQNDSSPSPVTDGQHVWAATGTGAIVAFDMEGNQLWKTNLQ
                     QMYGAFGQMFGYASSPLLYDGKLIVQVLHGYTTDEPSYLMAFDAVTGNVLWRVERWTD
                     AEVESPDAYTTPTLLHHEGTTQIVVLGGDYVTGHDPQTGEEIWRAGVTNPKKITNCRI
                     IASPVTAGGMIYAPTRKHPVTALRAGGQGDIATTHLAWQWTEFGGPDVPTPACDGRYF
                     YMVEDRGAATCLDAKTGAVIWGPERSARGTVSASPLLADGKLYFTNEDGVTTVLAAGP
                     KYEVLATNELPGIYVLSSMAVSGNRLFLRTATHLYCIGE"
BASE COUNT          346 a          586 c          604 g          408 t
ORIGIN      
        1 ttgaactgta cgctgaaacg accctgcccc ttcctgaaaa tccgtccaac acctttcgaa
       61 cggaggacac ggccattgtg gccctgcgcg tgcgtgaggg cgatgatgcc agctgtctga
      121 atctgaaccg ggcacaatca ccccggctcc tgggcgtgaa cccggacgag ttagccgcgc
      181 ttcgcgcgtt cctgccggaa ggcacacagg atgacttgtg gccgctgctc acgattgagc
      241 tgcacgacgg cgccgtgccc gccctggtgg gggatagcga caccgccttg tgggggctcc
      301 agaaaaagac cggcatcgaa aacggcgatg tcctcatcta ccgggacgat gacggcaacg
      361 aagtgactgt gagactggtg ggacgcctgc ccatgcgggt ttcggtattc caggggtcca
      421 ttcttatatc ggacgcctgg tttacccgcc tttttccctc cgaggccgga tttcgcgcct
      481 ttcttgtcga cacgccgccg gatcgggcgg atgccatcgc tgccgccctg aacaagacat
      541 ttgaacggga aggcatggat gccgaacccg ctccgcaacg ccttctccgc ttttatgccg
      601 tggaatccac ctatctcgcg atgtttctgg tgctcggtgg gctgggcatg gtgctgggca
      661 gcgcggctct ggggattgtg gtgttgcgca acttgttcga gcgtcgcagc gaaatcgcca
      721 tgctccgcgc ggtaggtttt gatcagcagg cgctgatccg gctgtttctc atggagtacc
      781 tggttctatt cggggccggt gtggcgattg gcggcgtggc agcggccatg gcgatgcttc
      841 ccgccgcggc ttcggctgga acttcatctc ccattggact ccaggcgctc atttttgcgt
      901 cgatcgcact gaccggcatc gtctgtatcg tcttcgccgt ccgggcaggc atcggccggg
      961 agaattttga cgcgctccgc accgaataag cgccgctact cgccaataca atacaagtgc
     1021 gttgccgtgc ggagaaaaag ccggttaccc gaaacagcca tcgaggaaag gacgtagata
     1081 cccggcagct cattcgttgc cagcacctcg tacttcggcc ctgccgcaag caccgtcgtg
     1141 acgccgtctt cattggtgaa gtaaagcttt ccgtcggcaa gcagtggtga agcgctcacg
     1201 gttccccgcg cactgcgctc cgggccccag ataacagcgc ctgtcttggc gtccaggcag
     1261 gtggctgcgc cacgatcctc gaccatgtag aaataccggc cgtcgcaggc gggagtgggt
     1321 acatcaggcc ccccgaactc ggtccattgc catgcaagat gcgtggtggc gatatccccc
     1381 tggccgccgg cacgcagggc agtcactgga tgtttgcgtg tcggcgcgta gatcatgccg
     1441 ccggcagtca cgggcgatgc gatgatgcgg caattggtga tctttttggg gttggttact
     1501 ccggcgcgcc atatttcctc gccggtttgc gggtcgtgcc cggtaacata atcgccgccc
     1561 agcaccacaa tctgggttgt gccctcgtga tgaagcaggg tgggtgtggt gtaggcatca
     1621 ggggattcga cctccgcgtc ggtccagcgc tcaacgcgcc acagcacgtt tccggtcacc
     1681 gcgtcgaagg ccatgaggta agagggctcg tcggtcgtat aaccgtggag cacctggaca
     1741 atcagcttgc catcgtacag cagcggcgac gacgcatacc cgaacatctg gccgaaggcg
     1801 ccatacatct gttgaaggtt cgtcttccag agctgattac cctccatgtc gaatgcaacg
     1861 atggcgccgg ttcccgtggc ggcccacacg tgctggccat cggtcacggg agatggagag
     1921 gaatcattct gtttttggcg gaac
//
LOCUS       DAOPBQ010000610         1935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_46525_length_1935_cov_4.896809, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000610 DAOPBQ010000000
VERSION     DAOPBQ010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46525_length_1935_cov_4.896809"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PKW60_14705"
     CDS             <1..472
                     /locus_tag="PKW60_14705"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPK00734.1"
                     /translation="PLIMLFANLPVFGWARPVPFNQQNLRSKRWGPVAVALAGPLSHL
                     AQLVVLIAGARLAFLVFHPDLENQGGGPWITLLWMLVQLNLILMLFNLIPVPPLDGHH
                     VLYAFLPPRGQHIMERIGPYGILIAVLVMRYGMGQPLVFLGRLAATLLFKGVAA"
     gene            578..1474
                     /locus_tag="PKW60_14710"
     CDS             578..1474
                     /locus_tag="PKW60_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPK00735.1"
                     /translation="MSITVVGSVALDTVETPAGRNEEGLGGAATYFSLSAAHYAQVHL
                     VGVVGEDFPQAHVDLLQAHGIHLEGLERVPGRTFRWAGRYHEDVNERDTLDTQLNVFE
                     NFHPRLPEPAREAPYLFLGNIHPSLQLEVLNQANPVFTALDTMNLWIDTTREELSQVL
                     ARTDAIIINDAEVKQLTGRANLIRGAQEVAALGPRVVVVKKGEHGCLLFAGGTVFSAP
                     AFPLENVVDPTGAGDAFAGGFMGFIAQKNRLDTATLRQAVIHGSVVASFVCEDFGPAA
                     LAALTAEGIAGRFEEFKALVDF"
     gene            complement(1647..>1935)
                     /locus_tag="PKW60_14715"
     CDS             complement(1647..>1935)
                     /locus_tag="PKW60_14715"
                     /inference="COORDINATES: protein motif:HMM:NF012626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HPK00736.1"
                     /translation="FADLPKTYVTEEIRIDTRESRKFDIVKQATRYFKDELKLKVITI
                     DGARIEFDDGWGLLRASNTSPALVMRCEANTPKRLKEIQKLIEQKVHELNK"
BASE COUNT          353 a          588 c          576 g          418 t
ORIGIN      
        1 cccgctcatc atgctgttcg ccaacctgcc tgtcttcggt tgggcgcgtc ctgtcccgtt
       61 caatcagcaa aatctgcgga gcaaacgctg ggggcccgta gcggtagcac tggccgggcc
      121 cttgtcgcat ctggcgcagc tggtggtgct gatcgccggg gcgcgcctgg cgttcctcgt
      181 gttccatccg gaccttgaaa accagggagg aggcccctgg atcacgctgc tgtggatgct
      241 cgtccagctc aaccttatcc tcatgctgtt caacctgata cccgtcccgc cgctggacgg
      301 ccaccacgtg ctctacgcgt ttctgccgcc caggggacag cacattatgg aacgaatcgg
      361 gccgtacggc atcctgatcg cagtgctggt catgagatac ggcatggggc agcccctggt
      421 cttcctgggg aggctggctg cgacgctcct tttcaaaggc gttgctgcct gacgccgggc
      481 tggggaactc tgcctttgcg tccggcattg gcgtttgcgc tcctgagtcg tgtacgatac
      541 cgcctcatca cgtcaacatt ctggggagac tggtcgaatg agcattaccg tggtgggctc
      601 tgtagcgctg gacaccgtgg aaacacccgc cggacggaac gaggaagggc ttggaggggc
      661 agccacctat ttctcgctgt ctgcggcgca ttatgcccag gtccatctgg taggcgtggt
      721 tggcgaggat tttcctcaag cccatgtcga cctcttacag gcacacggaa tccatctgga
      781 aggcctcgag cgtgtgcccg gaagaacctt tcgctgggca gggcgttacc atgaagacgt
      841 gaatgagcga gatactctgg atactcagct caatgtattt gagaatttcc acccgaggct
      901 gcccgaaccg gcgcgcgagg cgccgtatct gtttctcggc aacatccacc cttccttgca
      961 gctcgaagtg ctgaaccagg ccaatccagt attcacggcg ctggatacca tgaatctctg
     1021 gatcgacacc acgcgggagg agctcagcca ggttctcgcc cgtacggacg ccatcatcat
     1081 caacgacgct gaggtaaaac agctgacagg acgcgccaat cttattcgcg gcgcgcagga
     1141 agtcgccgca ctcggcccgc gcgttgtcgt ggtcaaaaag ggagagcacg gctgcctgct
     1201 ttttgcgggc ggtacggtgt tctctgcgcc ggcatttcct ctggaaaatg tcgttgaccc
     1261 caccggagcg ggggatgcct ttgcgggcgg ctttatgggc tttatcgcgc aaaaaaacag
     1321 gcttgacacc gccaccctgc gccaggcagt cattcacggc agcgtcgtcg cgtcgttcgt
     1381 atgtgaagac ttcggccccg ccgcactggc cgccctgacc gccgagggca tagccggccg
     1441 tttcgaggaa ttcaaagcac tggtagattt ttagcctttt tccaggcagt caaaagcgag
     1501 aaatatccct cgaatcaaca tgtccagaag tgcgccggca gcatggatcc ggccacaccc
     1561 cggccgcgca cgggggtctc cgcccagccg cccgggaaag gcacggcagt gcaccgggtc
     1621 aggggtgcga cctcatgcgc acccggtcat ttgttcagct cgtgaacctt ctgctcgatc
     1681 agcttctgga tttctttcag gcgctttggt gtattggctt cgcagcgcat gaccagcgcc
     1741 ggcgaggtgt tcgaggcgcg aagcagtccc cagccgtcgt caaattctat cctcgcgcca
     1801 tcgatggtga tgactttcaa cttcagctcg tctttgaaat accgggtcgc ttgcttcacg
     1861 atgtcgaatt tccgcgactc acgggtgtca atgcgtattt cctcagtgac gtaggtcttg
     1921 ggcagatccg cgaag
//
LOCUS       DAOPBQ010000611         1926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_46849_length_1926_cov_3.051309, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000611 DAOPBQ010000000
VERSION     DAOPBQ010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46849_length_1926_cov_3.051309"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /locus_tag="PKW60_14720"
     CDS             <1..1705
                     /locus_tag="PKW60_14720"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK00737.1"
                     /translation="LVWRESRRDRLLLWFALIYGLSLTNHSTMAMLGPVFALYVLALE
                     PKLWRRAGLLAQMAGLALLGFSVNLYLPIRSAANPPMDWGNPETLQGFWDVITRKQYQ
                     FMFTQYPRSLGRFAMQLHAFVKGYAREFTPVIAWLPMVGLASLWARSRSAALFFLSLF
                     ATVVLGFMLLLNYELTHESLWIMSTHWIPAYVIAGILMGAAIHALGRASKHPQWIQAG
                     VSAVCVVLPLLEHYEHNDRSYSRLALDYALNVLNTLEPDAVYIPTADHAIFPVIYLQV
                     VEGMRPDVFIGNKYGEIEPVFRNYLPGEEQARAGTFLPREQQNLALQRFIEATARPVY
                     FSHRSMIPPLPNHEAANAGVLYRIVPQGDSRSYAGVWRRYQWDFDELAASGIDGDWTD
                     AAIAFDYFFGLGRAFLDDGKTGDALDAFERALTAVGRQAAWANDAGSECARYGLLDAA
                     GMHFREALAEAPDAAYIRKNLARTLMMQGLWEEARRQLDTVLAAQPGDYAALRLSARA
                     LRQLGEFDLALAQLERAAELRPGEASIYREMSRIYAEDKHDDDAAQRFREKARSAAEG
                     Q"
     gene            complement(1735..>1926)
                     /locus_tag="PKW60_14725"
     CDS             complement(1735..>1926)
                     /locus_tag="PKW60_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00738.1"
                     /translation="VLLNNYRFAYTAWPTVAFDVPAEQVMEVDKRTGEVAPVIDDSPD
                     MEGLQVSLGAGDGRLFLLP"
BASE COUNT          335 a          562 c          641 g          388 t
ORIGIN      
        1 tctcgtatgg cgggaatcgc gccgggatcg gctgctcctc tggtttgcgc tgatatacgg
       61 gctgagcctg acgaatcaca gcacgatggc gatgctgggc cccgtgtttg cgctgtatgt
      121 gctggcgctg gagccgaagc tctggcgccg cgcgggcctg ctggcgcaga tggcgggcct
      181 ggcgctgctc ggattctctg ttaacctgta tcttccgatt cgttctgcgg cgaatccgcc
      241 catggactgg ggaaacccgg agaccctgca aggattctgg gacgtcatca cgcgaaagca
      301 gtaccagttc atgtttacgc agtatccgcg ctcgctgggc cggtttgcga tgcagttgca
      361 tgcctttgtg aaaggctatg cgcgcgaatt tacgccggtc atcgcctggc tgccgatggt
      421 gggtctggcg agcctgtggg cgcggtctcg cagcgcggcg ctgttctttt tgagcctgtt
      481 cgcgacggtg gtgttggggt tcatgctgct gttgaattac gaactgacgc acgagtcgct
      541 gtggatcatg agcacgcatt ggatccccgc ctacgtcatc gccggcattc ttatgggggc
      601 ggcgattcac gcgctcggca gggcttcgaa acatccacaa tggattcagg cgggcgtgag
      661 tgcggtgtgt gtggtgttgc cgcttcttga acattacgaa cacaacgatc gcagctactc
      721 gcgcctggcg ctcgattacg cgctcaacgt gctcaacacg ctggaaccgg acgcggtgta
      781 cattcctact gccgaccacg cgatctttcc ggtgatctac ctgcaggttg tggaagggat
      841 gcggcccgac gtctttatcg gcaataagta cggggagatc gagccggtgt tccgcaacta
      901 tctgccggga gaggagcagg cgcgcgcggg gacgtttctg ccccgtgaac agcagaatct
      961 ggccctgcaa cggttcatcg aagcgacggc ccgccccgtc tacttcagcc accgctccat
     1021 gatcccgccc ctcccgaacc atgaggccgc caatgccggt gtgctctacc ggatcgtgcc
     1081 gcagggagac tcccgctcgt atgcgggcgt gtggagacgg tatcaatggg attttgacga
     1141 attggcagcg agcgggatag acggcgactg gaccgatgcg gccatcgcgt ttgattattt
     1201 tttcggactg ggccgcgcct tcctcgacga tgggaagacg ggtgacgcgc tggacgcgtt
     1261 cgaacgggca ttgacggcgg tggggcgtca ggcggcctgg gccaacgatg ccgggtcgga
     1321 atgcgcgcgc tacggactgc ttgatgccgc gggaatgcat ttccgcgagg cattggccga
     1381 ggcgcccgat gcggcgtata tccgcaagaa cctcgcgcga acgctcatga tgcagggcct
     1441 gtgggaagag gcgcgcaggc agctggacac cgtgctggcc gcgcagcccg gcgattatgc
     1501 ggcgttgcgc ctcagtgctc gagctctgcg gcaactcggg gaattcgacc tggctctcgc
     1561 gcagctggag cgcgcggcag agctgcgtcc cggcgaagcc tcgatttacc gcgaaatgag
     1621 ccggatttac gccgaagaca agcacgatga cgatgcggca cagcgtttcc gggagaaagc
     1681 gcgaagtgcc gcggaagggc agtgacgccc cttatccacc agtgcgtgtg ccgtctaagg
     1741 caacaggaac aaacgtccgt caccggcgcc aagagatacc tgcaggcctt ccatgtcggg
     1801 gctgtcgtcg atcacggggg ccacttctcc ggtgcgttta tccacttcca tgacctgttc
     1861 cgccggcaca tcgaaggcga ccgtcggcca ggccgtgtag gcgaaccggt agttgttaag
     1921 taacac
//
LOCUS       DAOPBQ010000612         1921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_47011_length_1921_cov_3.923365, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000612 DAOPBQ010000000
VERSION     DAOPBQ010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47011_length_1921_cov_3.923365"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PKW60_14730"
     CDS             <1..680
                     /locus_tag="PKW60_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00739.1"
                     /translation="TIREKCIRDRAASDTFWYDLAADPGEQHSLSDVPPWGEALICQM
                     DHLDLRGRSGLHILVTRESGTSSHVRCQIDAGQLGAYSVFFPGGRGRACRSDTGVTFE
                     VDLKDREDRGPTGAWPPRLGNDSAHLFVEVPGDAPVAVSITRDGATVEPESVRVGNAL
                     VQMTLDGTLLNPGAIESTWAGMQSAESQQSFGIYVWYEAGAPRARRQELDPELVEAFR
                     GQGYLGD"
     gene            complement(694..1353)
                     /locus_tag="PKW60_14735"
     CDS             complement(694..1353)
                     /locus_tag="PKW60_14735"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPK00740.1"
                     /translation="MMTLSTVEQEILDQMLARRPDLEPCVDALLQLHSALVMCYDGGG
                     KLMLCGNGGSCADAMHIAGELCKSFERKRPLSAQLTAALQQLPHGEALAEHLEAGLPA
                     ISLGFNGSLKTAMENDSALRDIAFAQEALALGKPGDVLVGISTSGNAANCLMAMSAAK
                     ALGAVTVSLTGPRGGKMAEAADIAIKAPGDSTKVVQEAHLVLYHTFCALIEAHYFPEL
                     R"
     gene            complement(1353..>1921)
                     /locus_tag="PKW60_14740"
     CDS             complement(1353..>1921)
                     /locus_tag="PKW60_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00741.1"
                     /translation="QAAAAADLLYLVRRFELKAGWMAVPHPYKRFGLQAATYRLGVPL
                     TGHPMIGHDIIYLHPMNHCAALGRVAQRDFLTFAQSVSNLTNGVYLTIGSAVMSPMVF
                     EKSMSLSQNVAQQAGKQIENHFIMVVDLAESHWNWQQGEPPEDNPDYYLRYNKSFSRM
                     GGTMRYLSADNRDFLLALLHNLEQRSID"
BASE COUNT          355 a          567 c          615 g          384 t
ORIGIN      
        1 agaccatccg agaaaagtgt atccgtgatc gcgccgccag cgacactttt tggtacgatc
       61 ttgctgcgga tcccggcgag cagcattctc tatcagatgt cccgccttgg ggcgaggcgt
      121 tgatctgcca gatggatcac ctggacctgc gcggacggag cggtttgcac atcctcgtga
      181 cgcgggaatc gggaacgtcc tctcatgtcc gatgccaaat tgacgcgggc cagctgggag
      241 cgtacagcgt cttcttccca ggcgggcgcg gcagagcctg ccgttcggat actggggtaa
      301 cctttgaagt tgatttgaaa gaccgggagg atcgaggccc aaccggagcc tggccgcccc
      361 gcctgggcaa cgacagcgcc cacctgtttg ttgaagtgcc tggtgacgcg ccggtcgctg
      421 tgagtattac ccgggatggg gccacagtcg agccggagtc ggtccgtgtt ggcaacgccc
      481 tggtccagat gaccctcgat ggcacacttc tgaatcccgg ggctatcgag tccacgtggg
      541 ctggcatgca atccgcggag agtcagcagt cattcgggat ctatgtctgg tacgaggctg
      601 gtgcgccacg tgcccgtcgc caggagctcg acccggaact ggtggaagct ttccggggac
      661 aaggctatct cggtgactga gagctcccat gcttcagcgc agttcgggaa aatagtgggc
      721 ctcgatgagc gcgcagaacg tgtggtagag cacaaggtgc gcctcctgga ccactttggt
      781 ggaatcgccc ggcgctttga tcgcgatgtc ggcggcttcc gccatctttc cgccgcgcgg
      841 gcccgtgagt gaaaccgtca ccgcgcccag ggcctttgcg gccgacatgg ccatcaggca
      901 attggcggcg ttgcccgagg tagaaatgcc tacaagcaca tcaccaggtt ttccgagggc
      961 caacgcctcc tgggcgaagg cgatgtcgcg cagtgcggag tcgttttcca tggctgtttt
     1021 caacgagcca ttgaagccca gtgaaatggc cggcagcccc gcttccaggt gttccgcgag
     1081 cgcttcgccg tgaggcagct gctgaagggc cgccgtcaac tgggcggaaa ggggccgttt
     1141 gcgttcgaaa cttttgcaca gttcgccggc aatgtgcatg gcatcggcgc agctgccgcc
     1201 gttgccgcag agcatcagct tgccgccgcc gtcatagcac atgaccaacg cgctgtgcag
     1261 ctggagaaga gcgtccacac acggctcgag atccggccgc cgcgccagca tctggtccag
     1321 aatctcctgt tcgacagtcg acagcgtcat catcagtcga tgctccgttg ttcgaggttg
     1381 tgcagaaggg ccagcagaaa gtcgcggtta tccgcggaca gataccgcat tgttccgccc
     1441 attcgtgaga aggatttgtt gtagcggagg tagtagtccg gattgtcttc gggcggttcg
     1501 ccctgctgcc agttccagtg cgattccgcc agatccacca ccataatgaa atgattttca
     1561 atctgtttgc cggcctgctg ggccacgttt tgcgacaggg acatggattt ttcaaacacc
     1621 atgggcgaca tcaccgccga gccaatggtg agatagaccc cgttggtgag gttgctcacg
     1681 ctttgcgcaa aagtgaggaa gtcccgttgt gccacacggc ccagggccgc gcaatggttc
     1741 atcgggtgca ggtagatgat gtcgtgcccg atcatggggt ggccggtcag cggcacgccg
     1801 agccggtatg tcgctgcctg gaggccaaag cgtttgtacg gatgcggcac ggccatccag
     1861 cccgccttca gctcgaaccg ccgcacaagg tagagaagat ctgccgcggc cgcggcctgc
     1921 c
//
LOCUS       DAOPBQ010000613         1920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_47053_length_1920_cov_4.020912, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000613 DAOPBQ010000000
VERSION     DAOPBQ010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47053_length_1920_cov_4.020912"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PKW60_14745"
     CDS             complement(<1..723)
                     /locus_tag="PKW60_14745"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HPK00742.1"
                     /translation="MPQARDWDILRFKERYYERVWGGQKLKALFGKPFPDNVPVGEAW
                     LISDHPGDESVIDGGFWKGQTLQALLAADARALLGANARTTPAGRFPLLLKLLDAAES
                     LSVQVHPDDACAERLGEPDVGKTEMWSVLQAEPGSELIYGLKPEVGREAFSNAVANGD
                     IEPLLNRFPVQAGDSVFVSAGTVHAIGAGVVLAEIQQNSDLTYRIHDWGRLDSDGQPR
                     TLHLEKALEAIHFSPTPAEGVVS"
     gene            complement(759..1712)
                     /locus_tag="PKW60_14750"
     CDS             complement(759..1712)
                     /locus_tag="PKW60_14750"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPK00743.1"
                     /translation="MNEPVVVPIALPAPFRLGTVNVFLVKSTPLTLIDAGINTDEAFA
                     ALDAGFRKEGLALRDIEVVLLTHTHIDHVGLLGRLRTSANFTVYAHPDAITDRLSHEE
                     AQEKSYQTALAMMREFGTPDDVVQRAAAEQRMFREYAADAAVDQGLEDGQTVGPYTVY
                     HVPGHSAKDILFVDHERRIAFTGDHVLPSVTPNSLLPRPKLGQARPKGLVQYCDSLRK
                     TRSLDLDTCFPGHGGAIHDHRAVIDSLFKRVDGRNARVLNILGSQLLSVYEVARGLFP
                     GADAAQMYLQLTAAVGHLDLLEEEGRVAPEYRDGVLCFRAL"
     gene            complement(1709..>1920)
                     /locus_tag="PKW60_14755"
     CDS             complement(1709..>1920)
                     /locus_tag="PKW60_14755"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPK00744.1"
                     /translation="LQTVNLETRQPDAALVERSDTCAVPALALIAEHAVAIVLAQFFV
                     EQFGGDSVAQMKRSYGAYVAALSER"
BASE COUNT          381 a          562 c          608 g          369 t
ORIGIN      
        1 cgaaaccacc ccttccgcag gagttgggct gaagtggatc gcttccaggg ctttttcgag
       61 gtgcaacgtg cggggctggc cgtcgctgtc caggcgtccc caatcatgaa tccggtaggt
      121 gagatcgctg ttttgctgga tttcggccag aaccacaccg gcgccaatgg catggacagt
      181 ccctgccgaa acgaacaccg agtcgccggc ctggacgggg aagcggttca ggagcggttc
      241 gatatcgccg ttggccacgg cattgctgaa agcctcgcgg ccaacttcag gtttgaggcc
      301 gtatatcaat tcgctgccgg gttcggcctg gaggacagac cacatctcgg tcttgccaac
      361 gtcgggctcg ccgagacgtt ccgcgcaggc atcgtccggg tgcacctgaa cggacaggct
      421 ttcggcagcg tcgagtagtt tcaacaagag cgggaatcgt ccagcaggag tggtgcgggc
      481 attagccccg agcagggcgc gcgcgtctgc cgcgagaagc gcctgcaaag tctggccttt
      541 ccagaaaccg ccatcgatga cgctctcatc ccccggatga tcggagatga gccatgcctc
      601 gccgacaggg acgttatcgg gaaagggttt tccgaataaa gccttgagtt tttgcccacc
      661 ccatacgcgc tcgtaatagc gttctttgaa gcggagaata tcccaatccc gtgcctgagg
      721 catccgtcac tactccctgg ttttcgcgcg ccagccgttc agagcgcacg aaaacataaa
      781 acaccatccc ggtattcggg ggcaacccgg ccttcttcct cgagaaggtc gagatggcct
      841 accgccgcgg tgagctggag gtacatctgc gcggcgtccg cacccgggaa gagcccccgc
      901 gctacttcat agacagagag gagctgcgag cccagaatgt tgagcacgcg cgcgttgcgg
      961 ccgtccactc gtttgaacag cgagtcgatg acggcgcggt ggtcatgaat ggctccgccg
     1021 tgcccgggaa agcaggtatc gaggtccaga ctgcgggttt tgcgcaatga gtcgcagtat
     1081 tgcacgaggc ctttgggacg ggcctggccg agtttggggc gcggaaggag cgaattgggc
     1141 gtcaccgagg gcaacacatg gtcgcctgtg aaggcgatgc gccgttcatg gtccacgaag
     1201 agaatgtctt tcgccgaatg ccccgggaca tgatagaccg tgtacggacc aacggtctgg
     1261 ccatcctcga ggccttgatc gacagccgca tcggctgcat attcgcgaaa catccgctgt
     1321 tcggcggctg cccgctgcac aacgtcgtcg ggcgtaccga actcgcgcat catggccagg
     1381 gcagtctggt aggatttttc ctgagcctct tcatggctta aacggtccgt gatcgcatcc
     1441 ggatgcgcat agacggtgaa attcgcagag gtgcgcaaac gccccaggag tcccacgtgg
     1501 tcgatatgcg tgtgagtgag cagaacgact tcgatatcgc gcagggccag tccttctttc
     1561 cgaaatccag cgtccagcgc ggcgaaggct tcatcggtat tgatgcccgc atcgatcagg
     1621 gtaagcggcg tggatttgac gaggaagaca ttgaccgttc ccaggcggaa cggagccggc
     1681 aacgcaatgg ggacaactac cggctcgttc atcgttcaga cagggctgcc acgtaggcgc
     1741 cgtaactgcg tttcatctgc gccaccgaat ccccgccaaa ctgttccacg aagaattgcg
     1801 ccagaacaat ggctacggca tgctccgcga tgagcgccag ggccggcacg gcgcaggtgt
     1861 cgctgcgttc gaccagggcg gcatcgggtt ggcgtgtttc caggttgacg gtttgcaggg
//
LOCUS       DAOPBQ010000614         1919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_47101_length_1919_cov_2.556867, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000614 DAOPBQ010000000
VERSION     DAOPBQ010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47101_length_1919_cov_2.556867"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PKW60_14760"
     CDS             complement(<1..356)
                     /locus_tag="PKW60_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPK00745.1"
                     /translation="MERLLLSGDAAVALAALDCGVQLGVGYPGTPSTEILENFSRLGG
                     KAEWAPNEKVALEVGIGAAFGGAASLVTMKHVGLNVAADPLFTVAYTGVPGALVIVSA
                     DDPGMHSSQNEKDNRH"
     gene            complement(633..794)
                     /locus_tag="PKW60_14765"
     CDS             complement(633..794)
                     /locus_tag="PKW60_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00746.1"
                     /translation="MKHLKHVSREIPAHAQIFGCFDKEEHPSLGLSIREFFADPLGVI
                     RLHFDKEPQ"
     gene            1054..>1919
                     /locus_tag="PKW60_14770"
     CDS             1054..>1919
                     /locus_tag="PKW60_14770"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPK00747.1"
                     /translation="MLERMLESHSRILGGPEPHLLTPLAHLGVWAKVDKAPYDHVLAA
                     EAQKLFVNSLPDGEQDYWDACRAYSDTLYGRLLAASGKDILLDKTPAYALILPFMMRV
                     FPDARYVVLTRHPLATFSSYANSFFDGNYAEANQYNPILNRYVPAIAAFLRQEAVSHC
                     HVRYEDLVKDAETWMGRIYDYIGVPFEQETIDYGANKSGAKREGLGDPIGVAQHARPQ
                     TGSIKKWVAELAHDSGKRALMREVIASLDPEDLATCGYPLETLWRPLEEAGNSIAPPA
                     KPRLTRYRFQRKL"
BASE COUNT          418 a          593 c          529 g          379 t
ORIGIN      
        1 aaatgccggt tgtccttctc attctggctg ctgtgcatgc cggggtcgtc ggctgagacg
       61 atcaccagcg cgcccgggac ccccgtgtac gccaccgtga acagggggtc ggcagcgacg
      121 ttcagtccca catgcttcat cgtaaccagg gaggccgcgc cgccaaacgc ggcaccaatg
      181 ccgacttcca gggccacttt ctcgtttggc gcccattcgg ccttgccgcc gagcctggag
      241 aaattttcca ggatttccgt ggatggggtt cccgggtatc ccactcccag ctgtaccccg
      301 caatccaggg cggccagggc cactgcggca tctccactca ggagcaaccg ttccatcgat
      361 tccgacatcg aataatctcc ataacattgc cagtgaacag ctgcatggaa ggcgcgcagc
      421 gggacgggaa aaggtcacgt tcgactgtag cgcatgacct cccgggagtt gcaacagggc
      481 tcgcaccgca gacgcgtttc cgttgtgcag aaagcgtacc aaaaggaggg gtctgaatca
      541 accagaaatg aagcggcaga catcaaaaac gaaagctaac gcccctttcg gtggcgttag
      601 ctttcgataa atcgcagtgc tcaaacgcgc aatcactgtg gctctttgtc gaaatgcagc
      661 cggatgacac ccagcggatc agcgaagaat tcccgaatgc tcagaccgag gctgggatgc
      721 tcttccttgt caaagcaacc gaaaatctgg gcgtgcgcag ggatctctct cgatacgtgt
      781 ttcaggtgct tcataccttt tcctttcagc cgcgttactt accttccgcg tatggtccca
      841 cgttctcccc caacacatga tactatacca caagccggta tgaacaatac aagtattttt
      901 ttatccagtc cggacggcag ccggatagga tggcgcgggc agttgacatt ccccccctcc
      961 ggcactagag tcgagcccgg atatggcacc cgggggtaga ctatgcgcgt cccactgctt
     1021 tttatcatca gctcaccacg ctctggcagt accatgcttg agcggatgct cgaatcgcat
     1081 tccagaatcc tgggggggcc agaacctcat ctgttgaccc ctctcgccca tctcggggta
     1141 tgggcaaaag tcgacaaggc cccctacgac catgttctgg cggctgaggc tcaaaaactg
     1201 ttcgtgaata gcctgcctga cggcgagcag gattactggg atgcgtgccg ggcctacagc
     1261 gacacgctgt acggccgact actggcggcg agcggtaagg acatcctgct cgataagacc
     1321 ccggcgtatg ccctgatact ccccttcatg atgcgggttt tccccgatgc cagatacgtg
     1381 gtcctcacac ggcacccgct ggccaccttt tcgtcatatg ccaattcatt tttcgacggg
     1441 aattacgccg aggccaacca gtacaacccg attttgaacc ggtatgtgcc ggccatcgct
     1501 gcatttctca ggcaggaggc ggtaagccac tgccatgtgc gctatgaaga cctggtcaaa
     1561 gacgccgaga cgtggatggg ccggatatac gattacattg gggttccctt tgaacaggaa
     1621 accattgact acggcgcgaa caaaagcggc gcaaaacgcg aaggcctggg tgatccgatc
     1681 ggcgtagcac agcacgcacg cccccagacg ggctcgatca aaaaatgggt ggcggaactg
     1741 gcccacgact ccgggaaacg cgcgctgatg cgcgaggtga tcgcctctct tgaccccgaa
     1801 gaccttgcca cgtgcggcta ccccctggaa accctctggc ggccgctcga ggaggccgga
     1861 aacagcatcg ccccccccgc gaagccccgg ttaacgcgtt accggtttca acgcaagct
//
LOCUS       DAOPBQ010000615         1918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_47116_length_1918_cov_3.921632, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000615 DAOPBQ010000000
VERSION     DAOPBQ010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47116_length_1918_cov_3.921632"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PKW60_14775"
     CDS             <1..731
                     /locus_tag="PKW60_14775"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPK00748.1"
                     /translation="DLLEQIQAKLGLTRIPNRIECFDISTQQGDKPVGSMAVFENGAP
                     ARSRYRKYAIRQVEGQDDFAMMREVLLRRYRRAVEENDLPDLVLIDGGKGQLNVAVTA
                     LRDLGLEDLDIASIAKARAQGDRHSPERFFIPGRANPIVPRQDSPVVHLLARVRDEAH
                     RFAVTYHRKRRKKATLSTRLTEIPGVGEKRARLLLNHLGSLARIQEAPVETIAALPGF
                     GVATAQRIKEYLAENQTGTRAMGD"
     gene            733..>1918
                     /locus_tag="PKW60_14780"
     CDS             733..>1918
                     /locus_tag="PKW60_14780"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPK00749.1"
                     /translation="MGFLLATILWVIVLAVLFALLVNGYMVALMMRYPMPQWANELHA
                     VSTEDGWNIQLFRRKSSEAPGEPVLLVHGLAANRLNFEAPRGQAIVDTLVADGYDCWL
                     VELRSCESATPPDGRDLGDVSFDDYLYKDLPAAIDYIRATTGHDKVHWVGHSMGAMLL
                     YAYNAVFGSELLASGTALGAPAGFKNVTYAPPALLFRLNMAAPKTVRTFLKAVAPIAR
                     VFGPGFHAFPVNWNNVHPDIDTGVLFNMVEAVPPQVASELGEWLSTGNWRIRGRQVDI
                     TEKFGEAGLPLLMICGKGDPLTPEIPMRRFFDALPGKDKQFVLLSKKNEVSADYGHVD
                     LLFGKHGTEEVYLPALQWIQNHPIKKVRRRPARKSAAAAKTAPVKAAETAKTPARTKA
                     APA"
BASE COUNT          397 a          591 c          581 g          349 t
ORIGIN      
        1 gcgatctgct ggagcagatc caggcaaagc tcgggctcac ccgcatcccc aatcgcatcg
       61 aatgtttcga tatttcgacc caacagggcg acaaaccggt tggctcgatg gccgtattcg
      121 aaaacggcgc gcccgccagg agccggtacc ggaaatacgc gatcagacag gtcgaagggc
      181 aggacgattt cgccatgatg cgcgaagtct tgctgcggcg ctaccgccgg gccgttgaag
      241 aaaacgacct gccggatctg gtcctgatcg acggcggaaa aggccagctc aacgttgccg
      301 tgacagccct gcgcgatctc ggcctggaag acctggacat cgcgagcatt gcaaaggcgc
      361 gtgcgcaggg agacaggcat tcacccgaac gctttttcat ccccgggcgc gccaatccca
      421 tcgttccgcg gcaggacagc ccggtcgtgc atctcctggc gcgcgtgcgc gacgaggcgc
      481 accgatttgc cgtcacttac caccggaagc gcaggaagaa agccacgctc agcaccagac
      541 tgacggagat accgggtgtg ggtgaaaagc gcgcccggct gctcttgaac catctcggct
      601 ctttggccag aatacaagag gcgccggtcg agaccattgc tgccctgccg ggcttcggcg
      661 tggctacggc gcaacgaatt aaagagtatt tggcggaaaa ccaaacgggc acacgtgcca
      721 tgggggatta gcatgggatt cctgttggcg accattctct gggtgattgt tcttgctgtt
      781 cttttcgccc ttcttgtaaa cggctatatg gtggcgctga tgatgcgtta tcccatgccg
      841 caatgggcca acgaactgca cgccgtctcg acggaggacg gctggaacat ccagttgttc
      901 cggcgaaaaa gcagtgaggc gccgggcgag ccggtccttc tggtgcacgg cctcgcggca
      961 aaccgcctca atttcgaagc gccccgcggt caggctatcg tcgatacgct tgtggcggac
     1021 ggctacgact gctggctggt ggaattgcgt tcctgtgaaa gcgccacccc gcccgacggc
     1081 cgcgatctcg gtgatgtcag cttcgacgat tacctgtaca aagatttgcc tgcggcaatc
     1141 gactacattc gcgcgacgac cggccacgac aaagtgcact gggtgggtca ctcgatgggc
     1201 gcgatgctcc tgtacgccta taacgccgtc ttcggcagtg agctcctggc cagcggcacc
     1261 gcgctcggcg ctcccgcggg gttcaaaaat gtcacctatg ccccgccggc gctgctgttt
     1321 cggctcaaca tggctgcacc aaaaaccgtg cggacttttt tgaaggccgt cgctcccatc
     1381 gcgcgagtgt tcgggcccgg gttccacgcc tttcccgtca actggaacaa cgtccatcca
     1441 gacatcgaca cgggcgtcct cttcaatatg gtcgaggccg tgccgccgca ggtggccagc
     1501 gagcttggtg aatggttgtc tacgggcaac tggcggatac ggggacgcca ggtggacatc
     1561 accgaaaagt tcggcgaggc aggtctgccg ctgctcatga tctgcgggaa aggtgacccg
     1621 ttgacgcccg agatcccaat gcgccggttt tttgatgcac ttccgggcaa agacaagcag
     1681 ttcgtccttc tctcgaagaa aaacgaggtt tcggcggact acggccatgt cgacctcctg
     1741 ttcggaaaac acggcaccga ggaggtctac ctcccggccc ttcagtggat acagaaccat
     1801 cccattaaaa aggtccggcg gaggcccgca cgaaaatcgg ccgcggcagc aaaaaccgcc
     1861 cccgtgaagg cagccgagac cgcaaagacc ccagccagga caaaagcggc gcccgcaa
//
LOCUS       DAOPBQ010000616         1916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_47184_length_1916_cov_3.610962, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000616 DAOPBQ010000000
VERSION     DAOPBQ010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47184_length_1916_cov_3.610962"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="PKW60_14785"
     CDS             complement(<1..1311)
                     /locus_tag="PKW60_14785"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00750.1"
                     /translation="MTPLQAAMWVKLRVARHTVASIRYQSRLKVAVISVAVVLLWAGA
                     FIAFYAGLEWLQKLGLDPSGGGLNLGKLLMGSLLSVLTLALFLMLTFSNVLIAFSTFY
                     RTREMAFLIQSPLPAHSLFIARLAECIAFSSWASAYLGSPLILAYGLVTDASPALYLA
                     AALVFLPFVVIPAALGTMLTMFLARLFPVVKKWVLIGLAACAVTAVFFYLRDKFLAGD
                     ESAERILTLVFGAASRTQSPFLPSHWAAQAILTAAAGDYSRSAFDLLLLISNALFFSW
                     LAMQAAEILFYPGWSALKGLDHQRFRPLDRGLLGRVERLLRALPNPTRSLIIKDIKLF
                     WRDPTQWSQFLIFFGIMAVYVANLRNRSAMLDAEAYRTFIVSLNLGACSLITATLTSR
                     FVYPLISLEGQRFWLLGLAPLTFRQLVWQKYWMCVATTAVFSMAI"
     gene            complement(1308..>1916)
                     /locus_tag="PKW60_14790"
     CDS             complement(1308..>1916)
                     /locus_tag="PKW60_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK00751.1"
                     /translation="TTIKLLTGLLKPSSGRAIIGGHDIQQEPVAAKQLLGYIPDTPFL
                     YEKLTGREFMRFVSGLYQLPEASAAKRSEELLELFEIREVGDQLIEDYSHGMRQKLSF
                     ASCFLHEPQVVVVDEPWVGLDPKNIRFVKNFLRERARQGSTIFMSTHSLSVIEDTTDR
                     IGIIHNGALLHIGSLSEIKALSRNPGSLEDVFLELTNDESTS"
BASE COUNT          437 a          489 c          653 g          337 t
ORIGIN      
        1 gatggccatg gagaacacgg cggtggtggc gacgcacatc cagtacttct gccagaccag
       61 ttggcgaaac gtgagcgggg cgagcccgag aagccagaag cgttgtcctt ccaggctgat
      121 gagggggtac acgaatcggc tggtcagggt cgcggtgatc agcgaacagg cgccgaggtt
      181 caggctcacg atgaatgtgc ggtaggcctc ggcgtcgagc atggcggagc ggttgcgaag
      241 gttggccaca tagacggcca tgattccgaa aaagatgagg aactgggacc attgggtggg
      301 gtctcgccag aacaatttga tgtctttgat gatcaggctg cgtgtggggt tgggcagtgc
      361 acgcaggagg cgctccaccc tgcccagaag gccccggtcg agcggccgga aacgctgatg
      421 atcgagcccc ttgagcgccg accagccggg gtagaagagg atttcagcgg cctgcatggc
      481 gagccaggag aagaagagcg cgttggagat caagagcaat agatcgaacg cgctccggct
      541 gtaatctcct gccgccgcgg tcaggatggc ctgtgcggcc cagtggctcg ggagaaacgg
      601 tgattgcgtt cgggatgccg ccccaaacac gagtgtgagg atgcgttcgg cggattcgtc
      661 gccggcgagg aatttgtccc gaagatagaa gaagacggct gtgacggcgc aggcggccag
      721 gccgatcagc acccattttt tcacgacggg aaagaggcgc gcgaggaaca tggtgagcat
      781 cgtgcccagc gcggcgggta tgacgacaaa cggcaggaaa accagtgcag cggccaggta
      841 aagcgcgggc gacgcatcgg ttaccagccc ataggcgagg atcaagggcg agcccagata
      901 cgcggacgcc caggaactga acgcgatgca ttccgccagg cgcgcgatga agagcgaatg
      961 ggccggcaac gggctctgta taagaaaggc catctcacgg gtccggtaga aggtcgagaa
     1021 tgcgatgagc acgttggaga acgtcagcat gagaaagagg gcgagagtca agacggatag
     1081 caggctgccc ataagtaatt tgccgaggtt cagtccgcca ccggaaggat ccagaccgag
     1141 tttctgaagc cactccaatc cagcatagaa ggcaatgaag gcgcccgccc agagcagcac
     1201 cacggcgaca gagatgaccg caaccttgag ccgcgactgg tagcgaatgg atgcgacggt
     1261 atgccgcgcc acgcgcagtt tgacccacat tgcggcctgc agcggcgtca tgacgtgctc
     1321 tcgtcgttgg tcagttcgag gaaaacatct tcaagagagc cgggattgcg gctgagtgcc
     1381 ttgatctcgc tcagcgagcc gatgtggagc aaggcgccgt tgtgaatgat gccgatgcgg
     1441 tcggtcgtgt cttcaatcac gctgagggaa tgcgtggaca tgaaaatagt gctgccttga
     1501 cgggcgcgtt cacgcaggaa gttcttgacg aaacggatat tcttgggatc gaggccgacc
     1561 cagggctcat cgaccacgac gacctggggt tcatgtagaa aacacgaggc aaacgacaat
     1621 ttttggcgca taccgtggct gtaatcttcg atgagctggt cgcccacttc ccgaatttcg
     1681 aatagttcga gcaattcctc gctgcgtttt gccgcggaag cctcgggaag ctgatagagg
     1741 ccgctcacaa atcgcatgaa ctcgcggccg gtgagctttt cgtaaagaaa cggcgtgtcg
     1801 gggatgtagc ccaggagctg ttttgccgct acgggctctt gctggatgtc gtgacctccg
     1861 atgatcgccc tgccggaaga aggtttgaga aggccggtaa ggagtttgat ggtggt
//
LOCUS       DAOPBQ010000617         1891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_48123_length_1891_cov_3.886710, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000617 DAOPBQ010000000
VERSION     DAOPBQ010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48123_length_1891_cov_3.886710"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKW60_14795"
     CDS             <1..329
                     /locus_tag="PKW60_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00752.1"
                     /translation="DLRMSQMHELAAFINRAALKTVPAIAVGDFNVRATEPHYHTLIE
                     EAGLERVMTLDTSIDHIFAVRNDGYTFEVLDTIPIKGEVSVPGGTVGFSDHTGYMSTV
                     RIRSTG"
     gene            575..1297
                     /locus_tag="PKW60_14800"
     CDS             575..1297
                     /locus_tag="PKW60_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPK00753.1"
                     /translation="MVPMFADSTFLLVIPALILSLWAQWKVKSTYAKYAQVANRRGIT
                     GAEVAQHILRDAGVALAGESGGLGAGVSLEPVAGHLTDHYDPRTRTLRLSQDIFYGRS
                     VAALGIAAHEVGHAVQHASGYAPLQARNFIYPVCALGSTLAFPLFVIGMFLGPGTGQL
                     VMQLAILLFTAAVGFTGLTLPVEFNASRRAVKALASGGYLTGDELAGARRVLSAAAMT
                     YVASTAMALLQLLRMLLLANRR"
     gene            1510..>1891
                     /locus_tag="PKW60_14805"
     CDS             1510..>1891
                     /locus_tag="PKW60_14805"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPK00754.1"
                     /translation="MRLDKLTIKAQEALSAAMDAAAQHQHPEIGSLHILHALVSQEGG
                     AVPSILQRLGLRPDTVLAATEEKLQSLPRISGAASQAGLSGAANRLLEAAWNEAQALK
                     DQYLSTEHILLGMISLRDDPAGILL"
BASE COUNT          372 a          603 c          528 g          388 t
ORIGIN      
        1 aggatcttcg aatgagccag atgcatgaac tggccgcctt catcaaccgg gccgcgctca
       61 agacagtgcc cgccatcgcg gtcggcgact tcaatgtccg ggccaccgaa ccccattacc
      121 atacgctcat cgaggaagcg ggcctggaac gcgtcatgac cctcgacaca tccatcgacc
      181 atatctttgc ggtccggaac gacggctaca ctttcgaagt gctcgatacc atccccatca
      241 aaggcgaggt ctccgtgccc ggaggaacgg tcggattcag tgaccacacg ggatatatgt
      301 ctaccgtacg gatccgcagc acaggctgat caggcgccaa tatgggccgg ttctcgcctg
      361 ttttgcacca gcacgtctca ttttgaggcg ctcccgggct gaagattgcg attcgaccgg
      421 cctcccgaag tcataaacca ttcatttaca ctatattacg cctattttag cgtttcttct
      481 ttcaaggcat gatacttgct tttcctttgg gcgagttgct atcgcgagac gatggcgcgt
      541 atactccctg aaagactgag gagactgtat tatgatggtg ccgatgtttg cggattcgac
      601 gtttctactg gtaattccag cgttaattct gtcgctctgg gcgcaatgga aggtcaaatc
      661 gacctacgca aaatacgcgc aagtcgccaa tcgcagaggg attacaggcg cggaagtcgc
      721 gcagcacatc ctccgcgatg cgggcgtcgc cctggcgggt gaatccggcg gacttggcgc
      781 cggggtgagc ctcgagcccg tagccggcca cctgacggac cactacgacc cccgcacgcg
      841 cactttgcgg ctttctcagg acatttttta cggacgcagc gttgccgcgc tgggcattgc
      901 cgcgcatgag gtcggccatg ccgttcagca cgccagtggg tacgcgccgc tgcaggcccg
      961 caacttcatt tatcccgtat gcgccctggg ctcaactctg gccttccccc tgtttgtgat
     1021 cggcatgttt ctgggacccg gcacaggcca gcttgtcatg cagctcgcga tccttctgtt
     1081 taccgcagcg gtcgggttta ccgggctgac cctccccgtc gagttcaacg cgagccggcg
     1141 cgccgtaaaa gccctggcca gcggcggata cctgaccggc gacgaactcg ccggcgcgcg
     1201 cagagtgctg agcgcggcgg ccatgaccta tgtcgcctcc accgcaatgg ccctcctcca
     1261 gctcctgcgt atgctgctcc tggccaaccg gcgctagccc aaccattcca gcattcccgc
     1321 cgacgcccgg ccttcacgcc gggcgtcgtt ctatttgtag caaaatgaaa caggtccgtg
     1381 ccggattgga gcacgccgcg ccgaaattcc gctctaagcc cttacgcatc aagccttccc
     1441 gttggcataa acgttgctct cttactcagg cgaagatgat ttcaacgcgc ctacagagag
     1501 gagcatccca tgcgattgga taaattgacg atcaaagccc aggaagcctt gtcggcggcg
     1561 atggatgccg ccgcacagca ccagcatcct gaaattggct ctctgcacat actccacgcc
     1621 ttggtgtcgc aggaaggtgg agccgtacct tcgatcctgc aacgattggg tctgcggccg
     1681 gatactgtgc ttgccgcaac ggaagaaaaa ctgcagtcac taccccgaat ctcgggggcc
     1741 gcctcgcagg cgggcttaag cggggccgcg aaccgcctgc tcgaggctgc ctggaacgaa
     1801 gcacaggcgc tcaaagacca gtacctcagc acggagcaca tcctgctggg aatgatttcg
     1861 ctgcgcgacg acccggcggg cattctcttg c
//
LOCUS       DAOPBQ010000618         1871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_48850_length_1871_cov_3.243392, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000618 DAOPBQ010000000
VERSION     DAOPBQ010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48850_length_1871_cov_3.243392"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1193
                     /gene="miaA"
                     /locus_tag="PKW60_14810"
     CDS             261..1193
                     /gene="miaA"
                     /locus_tag="PKW60_14810"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPK00755.1"
                     /translation="MHTEGVNMLVVLGPTASGKTALGVYLADILGGEIISADSRQVYR
                     GLDIGSGKDLGEYVVNGRTIPYHLIDIVDLSHEFSVFEYQQRCFAAFEAISGRGALPV
                     MVGGTGLYIEAVLRGYRMVEAPEDARLREELSCLSDEALAARLVALKPGLHNTTDLTD
                     RNRLVRAIEIAEYTKCHPPAPAPSIRPFILGTRWDRKILRHRIGKRLQQRFDAGMIEE
                     VEGLRAQGVPWEKLHFLGLEYRYIADYLQGRIATRDELYEGLWTAICQFAKRQETWFR
                     RMERNGAVIHWIDNAAPETALELLRTRDAAIRPL"
     gene            1332..1778
                     /locus_tag="PKW60_14815"
     CDS             1332..1778
                     /locus_tag="PKW60_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00756.1"
                     /translation="MSSQQMPEGVDLLVSGAWLLFFLVMMAVSIAIAIAVILIVQSFY
                     KRIPSEHRKMEPGMVWLLIIPCFSIVWNFFVFLRLSDSFKSYFDSQGIEDVGDCNRTL
                     AMAYCIAAVLCLVPCLNYVAGPVSLVLLVLALVKYNELKNRIPAAF"
BASE COUNT          361 a          524 c          584 g          402 t
ORIGIN      
        1 ggcctgagag acaggcgcgt cccacgattt ctggagcagg tccggaggca gggggggcac
       61 aaactccccc ggcgcgctgt tttcggccgc agctgcggat gttaaagcaa gaacaatgat
      121 cacgagcaag aagtagtggc gcatcaagat tcctccggtt ggactctacg tattatactc
      181 aatgcggcca cgcaccgttt catcggcctg cttccctcgc gcaccgcgag atctgcgctc
      241 cggtgcgtta tgatacgggc atgcacacag aaggcgtgaa catgctcgtg gtattgggcc
      301 ccaccgcctc cgggaagacc gcgctcggcg tctacctggc ggatatcctg ggcggtgaaa
      361 tcatctcggc cgattcgcgg caggtctacc gggggctgga catcggctcg gggaaagatc
      421 tgggcgagta tgtggtcaat ggccgcacaa tcccgtacca tctcatcgat atcgtcgatc
      481 tctcccacga attcagcgtg ttcgagtatc agcagcgatg ctttgccgca tttgaggcga
      541 tctcgggccg gggcgcgctc cccgtcatgg tgggagggac gggactctac atcgaagcgg
      601 tgctgcgagg ctaccggatg gtcgaggcgc cggaagacgc ccggctgcgc gaggaattga
      661 gctgcctgtc cgatgaggct ctggcggccc ggttggtggc gctcaaaccc ggtctccaca
      721 acacgacaga cttgactgat cggaaccggc tcgttcgcgc gattgagatc gccgaatata
      781 cgaagtgcca tccgcccgcc cccgcgccct ctatccggcc atttatcctg ggcacgcgat
      841 gggaccggaa aatcctgcgg catcgtatcg gcaagcgcct ccaacagcgg tttgatgccg
      901 gtatgatcga ggaagtcgaa ggtttgcggg cccagggagt gccctgggag aaactgcatt
      961 ttctggggct ggaataccgc tatatcgcgg attatctcca ggggcgtatc gccacccggg
     1021 acgagctgta tgaagggctg tggacggcga tttgccagtt tgccaaacgg caggagacgt
     1081 ggtttcgccg catggagcgt aacggagcgg tcatccactg gatcgacaac gccgcgccag
     1141 aaacggcgct cgagcttctg cgcacccgag acgctgcaat tcgtccgctc taaccatttc
     1201 aggcagagcc cggatccggc ggccgtggtc cttcaatcgg ccaaagaagg ctgcttttcc
     1261 atttacatcc ggcgcgaaaa ccggtatttt ggcggtaacg ggaggtctta acaggcaggg
     1321 aggggtttcc tatgtcaagc cagcagatgc cggaaggtgt ggatttgtta gtgtcggggg
     1381 cgtggctgct cttctttctc gtgatgatgg cggtgagcat tgccatagcc atcgcggtca
     1441 ttctgatcgt gcagagcttc tacaagcgca ttccatctga acaccggaaa atggaacccg
     1501 ggatggtctg gctgctgatc attccgtgtt ttagcatcgt ctggaacttc tttgtgtttc
     1561 tccggctgtc tgactccttc aagagctatt ttgacagcca gggtatcgaa gacgttggcg
     1621 attgcaaccg cacgctggcc atggcctatt gcattgccgc ggtgctgtgc ctcgtgccat
     1681 gtctgaatta tgttgccggg cccgtgtcgc tggtgttgct ggttctcgca ctggtgaaat
     1741 acaacgaact caaaaaccgc attccagccg cgttttgacg aaacaatctc gtgaaggatc
     1801 cgtgacggta gtgcgtatgc cggcggcgca agaacgccat tcccgtggac ctgtgagcgc
     1861 actggcgggt g
//
LOCUS       DAOPBQ010000619         1866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_49017_length_1866_cov_3.699061, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000619 DAOPBQ010000000
VERSION     DAOPBQ010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49017_length_1866_cov_3.699061"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="PKW60_14820"
     CDS             complement(<1..1747)
                     /locus_tag="PKW60_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011118143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HPK00757.1"
                     /translation="MTRELGFTIPAMLLAIVVAHAGQEGVAFFAGDGVPFDGTTVYEA
                     PLEEIPIPPQSLTVMAWVKLDETGGSQVFLNTGAANEGFTFYLYDGRVRMLVRNQTES
                     YACALGPAPKRGEWTHYAGTYDGEHAAVYMNGTLCEIKECPGQLTKLGEVLSLGGLDY
                     GQRAVKGWLGDLRIYDRALSGEEIAAAAGGSLSPPPMVRWTDSGREGASWTAEGNAVV
                     ARRMDGRRVLLNRREDGYRGIWYFNQKSDDEYVYKYSGGLGTYCAKHIPFAWYAPQVN
                     KTFFTYGGTHKNENRLIHMVSYFDHATGEVPRPLLLLDKQTTDAHDNPVINIDPEGYI
                     WIFSSSHGTGRPSFISRSTEPWAIDAFELKWEGNFSYPQPWIIPGKGWLFMHTHYTGG
                     RAICMSTSPDGVNWTDRRFLSRMAQGHYQITNFFGQKIGSAFNYHPPEKGLNWRTNLY
                     YMESDDFGETWKTAAGEPLELMLTEPDSPCLVAEYESQGLNVYMKDINFDSAGRPVIL
                     YLTSKGYESGPQNMPRTWTIAHWTGQQWQILPSNIISDNNYDMGSLYIETDAQWRIIA
                     PTETGPQPYNPGGEVA"
BASE COUNT          364 a          552 c          532 g          418 t
ORIGIN      
        1 tggccacttc accgcccggg ttatacggtt gcgggccggt ctcggtgggg gcaattatcc
       61 gccattgagc gtcggtttca atatacagtg aacccatgtc gtaattgttg tcggagatga
      121 tgttggacgg cagaatctgc cattgctggc cggtccaatg cgcgatggtc caggtgcgcg
      181 gcatgttttg ggggcccgat tcgtagcctt tgctcgtaag gtacaggatg acggggcgcc
      241 cggcagaatc gaaattgatg tccttcatgt agacattcaa accctgagat tcgtactcgg
      301 ccacgaggca ggggctgtca ggctcggtca gcatcagctc caggggctcg cccgcggctg
      361 tcttccacgt ctcgccgaaa tcgtcggatt ccatgtaata gaggtttgta cgccagttca
      421 ggcctttttc agggggatgg tagttgaatg cgctgccgat cttctgtccg aagaagttcg
      481 ttatctgata gtgcccctgg gccatgcgcg agaggaagcg gcgatccgtc cagttgacgc
      541 cgtcaggact ggtggacatg cagatggcgc ggccgccggt gtagtgggtg tgcatgaaca
      601 accacccttt gcccggaata atccacggct ggggataaga aaagttgccc tcccacttca
      661 gctcgaacgc atcgatggcc cacggctcgg tgcttcggga gatgaacgag gggcgccccg
      721 taccgtggct gctcgagaaa atccagatgt agccctccgg gtcgatgttg atgacggggt
      781 tgtcgtgggc gtcggtggtt tgcttatcca ggagcaacag agggcgcggc acttcgcctg
      841 tcgcatgatc aaaataggag accatgtgga tgagccggtt ttcgttcttg tgcgtgccac
      901 catacgtaaa gaaggttttg ttgacttgcg gcgcgtacca ggcaaagggg atgtgtttcg
      961 cgcagtaagt ccccaaaccg ccgctgtatt tgtagacata ctcatcgtca gatttttgat
     1021 tgaaatacca gatgccgcgg tagccgtcct cgcgccgatt caaaagtacg cgcctgccgt
     1081 ccatcctgcg ggccacgaca gcgttccctt ctgcggtcca ggaggcgcct tcgcgcccgg
     1141 aatcggtcca gcgcaccatc ggtggtggcg aaagactccc gcccgccgcc gcggcgatct
     1201 cttccccgga cagcgcgcgg tcatagatcc gcagatcgcc cagccaaccc tttaccgcgc
     1261 gttgcccgta atccaagccg ccgagggaca gcacttctcc cagctttgtc agctggcccg
     1321 gacactcctt gatctcgcat aaggtaccgt tcatgtatac cgctgcgtgt tcaccgtcgt
     1381 aggtgcccgc ataatgagtc cattcgcctc ttttcggtgc agggcccagc gcgcaggcat
     1441 agctttccgt ctggttcctg accagcatac gcacccgtcc atcatagaga tagaatgtga
     1501 atccctcgtt ggccgcgccg gtattgagaa agacctgtga accgcctgtc tcatccagct
     1561 tcacccaggc catgacggtc aacgactgcg gaggaatcgg gatctcttca agcggcgcct
     1621 catacaccgt agttccatcg aacggcacac cgtcaccggc aaagaaagcc actccctcct
     1681 gccccgcgtg cgccaccacg atagccagaa gcatcgcagg aatagtgaat cccaattctc
     1741 tagtcatgtc ctgtcccccg ttgcgatctc ggctggagaa agacttcgtc tcaccgcagc
     1801 tcaattttgc gcaagttctt ccgtcaattc ctcccattct tcgtacagtg aggccagatc
     1861 agcttt
//
LOCUS       DAOPBQ010000620         1857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_49372_length_1857_cov_3.674806, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000620 DAOPBQ010000000
VERSION     DAOPBQ010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49372_length_1857_cov_3.674806"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="PKW60_14825"
     CDS             complement(<1..1528)
                     /locus_tag="PKW60_14825"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPK00758.1"
                     /translation="MKKLLFRFAFALVLCPLLAALGCATAGTPSRRGVQTFEPVPHGR
                     VGRQELPTPPAQRATDLLRAAGDAFRKANEAQENGDEKAALRHYAKMLQLLQEADLDP
                     AIFYNLRPEFERILAQTTAQAGLMDSEGHLERLTPEEMAEAGLGGGIEIPFPLPERVL
                     AEIEEIQQVYAKNFQYGLNRSVKYDQHLRRELTLAGLPEDLVWLAMVESQFHPTVVSH
                     AGAAGMWQFMPATGRRYNLRINSYVDERFNWRKETNAAVAYLTELRDFFEGDWPLAIT
                     AYNMGENGMERVIAMNGGERDLWKLLETSPGCDRMARESQKFYAKFLASIIVGKNPEK
                     YGFTIPSGTLEEAEFVPIEGSYNLASLEKAAGWPEGTLRDLNRDLTRGITPPAGEHML
                     VVPKHGAKTLLAALESAARVQPNVHVVKRGETPDGIAKHYGVSVRELMAVNKVRSPLH
                     LPVGMNLMIPGTGSVPVPDAPAAPAVSETRETHAAATYRVKKGDTLSGIATRFGAKVS
                     D"
BASE COUNT          342 a          533 c          591 g          391 t
ORIGIN      
        1 tgtcgctcac tttggctccg aaccgggtag caattccgga cagggtatcg ccttttttga
       61 cgcgataggt ggctgcggca tgcgtttcac gggtttcgga tacggccggg gcggcgggcg
      121 cgtccgggac cggcacgctg ccggtaccgg ggatcatgag gttcatccca accgggagat
      181 ggagtggcga gcggaccttg ttgacggcca tcagctcgcg cacggagacg ccgtagtgtt
      241 tggcaatgcc gtccggggtt tcgccccgtt tcaccacgtg gacgttgggt tgcactctgg
      301 cggcgctttc gagtgcggca aggagggtct ttgccccgtg tttggggact acaagcatgt
      361 gttcaccggc agggggcgtg atgccgcgcg tgaggtcacg gttcaaatcg cgcagggtac
      421 cttcgggcca gccggcggcc ttttccagcg aggccagatt atagctgcct tcgatgggca
      481 caaattccgc ctcttcgagc gtgccggagg ggatggtgaa cccgtatttc tcaggattct
      541 tgcccacaat gatggatgcg agaaacttgg cgtagaattt ttgcgattca cgggccatac
      601 gatcgcaacc gggagacgtc tccaggagtt tccagagatc gcgttccccg ccattcatgg
      661 cgatgacgcg ctccatccca ttctcgccca tgttgtacgc ggtgatggcg aggggccaat
      721 cgccctcgaa aaagtcgcgc agttctgtca ggtaagcgac ggcggcattg gtttctttgc
      781 gccagttgaa acgctcgtcg acgtaggaat tgatgcggag gttgtagcgg cggccggtcg
      841 cgggcatgaa ctgccacatg ccggctgcgc cggcgtgcga cacgacagta ggatgaaact
      901 ggctttcgac catggccagc cagacgagat cttcgggcaa tccggccagt gtgagttcgc
      961 ggcgcaggtg ctggtcgtat ttgacgcttc ggttcaaacc atactgaaaa ttcttcgcat
     1021 agacctgctg aatctcctcg atctctgcga ggacccgctc tggcagtggg aacgggattt
     1081 cgatgccgcc gcccagcccg gcctcggcca tttcttcggg cgtgagccgc tcgaggtggc
     1141 cttcgctgtc catcagcccg gcttgtgcag tggtctgggc gagaatacgc tcaaactcgg
     1201 ggcggaggtt gtaaaaaatc gcagggtcga ggtcggcctc ctgcaacagc tgcagcatct
     1261 tcgcgtaatg gcgcaacgcg gccttttcgt cgccgttctc ctgggcttca ttggctttcc
     1321 ggaaggcatc gccggcggct cggagcaggt cggtcgcccg ttgcgcggga ggagtgggga
     1381 gttcctggcg gccgacccgg ccatgcggca ccggttcgaa ggtctggacg ccgcgcctgc
     1441 tgggcgtacc cgctgtggcg cagccaagcg cggcaagaag agggcaaagc acaagtgcga
     1501 acgcaaagcg aaacaacagt tttttcacga atatatcccc aaaaagtcgt atcctgcctc
     1561 tcgtacaaga cgggaaattt tctccccagc ccatagtgta gataacaacg tttagtgtgt
     1621 caagatattg tgtaccccaa cagagtgtgt ataagtgaaa aaaagagaaa aaccggacgc
     1681 tgaaaacgcc ggttttgaac gccccctgca attgatcggg ggctgctggc cgcgccgttg
     1741 acggcgctcg catcgtatgg cggctctgtc tcttctctcc agcatacgac tcgcagcccg
     1801 cccgctcgcc cgaagcacgg cccctggcca gtgccccgtg ctgctgtatc tcgtgca
//
LOCUS       DAOPBQ010000621         1843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_49939_length_1843_cov_3.789709, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000621 DAOPBQ010000000
VERSION     DAOPBQ010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49939_length_1843_cov_3.789709"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..713)
                     /gene="msrB"
                     /locus_tag="PKW60_14830"
     CDS             complement(294..713)
                     /gene="msrB"
                     /locus_tag="PKW60_14830"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPK00759.1"
                     /translation="MSKDSDGVEKVEKTDAEWRAQLRPEQFCVVREKGTERAFTGAYW
                     DNTRAGEYRCVGCGLPLFSSSAKYDSGSGWPSFFQPVNDGHIATKTDTSHGMLRTEVL
                     CARCDAHLGHVFEDGPEPTGLRYCINSASLTFEEKNP"
     gene            complement(981..1583)
                     /locus_tag="PKW60_14835"
     CDS             complement(981..1583)
                     /locus_tag="PKW60_14835"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK00760.1"
                     /translation="MDIEIREYRPEDEAQWMRTHAIIMSISHAWNYTIQQRPVYKRPS
                     TCLVAMADGALIGLTDIEYDTEPGELCFLKDSCGGYVLEFGRLPEYAGHQLGAKLIAA
                     SVEASRQKGIYRLEYWTQDRNAARFYQRFGMKEIGRHCRFRMKPTEEVEELLGKKYIG
                     IEYLYACCLVEEFPLIKQKYTVLEDHPLEPHVCIGYELRF"
BASE COUNT          354 a          598 c          501 g          390 t
ORIGIN      
        1 gaagcccatc atggtggtcc ccagagaagg tgtgatatct tcgcgcgcga atcccgccag
       61 catcgtggcg gccttcccgc cgggttgaga cgatgtctgg ccctgggctg cccccacggt
      121 cgcaaacgag gcggccgccg ttcccaaaaa agtgcgtcgt ttcattccct caattcctcg
      181 ctgcatcgat tgcaccagaa aacacgtcct taacgcgctt cgattctact acatttgcgg
      241 ccggcaacgt acgtgcagca gcactctttt accgcagcac agttcgcgcg gcattacggg
      301 ttcttctcct cgaacgtgag tgacgccgaa ttgatgcaat accggaggcc cgtgggttcg
      361 gggccgtcct caaacacgtg tcccaggtgt gcatcgcagc gtgcgcacag gacttccgtt
      421 cgaagcatcc cgtggctcgt gtcggtcttt gttgcaatat ggccgtcgtt cacgggttga
      481 aagaagctcg gccagccgga tcctgaatcg tacttggcgc tggagctgaa caggggcaag
      541 ccacagccca cacagcggta ctcaccggcc ctggtgttgt cccagtacgc gccggtaaac
      601 gcgcgttccg tgcccttttc acgcacgaca caaaactgct ccggcctaag ctgtgcccgc
      661 cactccgcat ccgttttctc gaccttttct acaccatccg agtccttact catcgtgtgc
      721 tctccgattg aagaggatcc cagtgcagac tttccgggtt catggggcgg ccaggccccg
      781 ccgcacccca aggcggccat cactccgatt accccagcga gatacagctt caaaagcaat
      841 cctcctattc tgccggcaca agacctcctg aagtgaaccg gaatcctatg agccactatc
      901 cctggtaaat gaaaatccgg actggcccgg ccacgcagtt ccggccattg ctgagcaccc
      961 gggatccatc gcgaaacacg tcagaaccgc agctcgtagc cgatgcagac atggggttcg
     1021 agggggtggt cctcgagtac cgtatacttt tgctttatga gaggaaattc ctctacaagg
     1081 cagcatgcat acaggtattc gatgccgatg tactttttgc cgagcagttc ttcaacttct
     1141 tcggtcggct tcatgcgaaa ccggcaatgc ctgccgatct ccttcatgcc gaagcgctgg
     1201 tagaagcggg cggcgttgcg gtcctgcgtc cagtactcga gccggtatat gcccttttgc
     1261 ctcgacgcct cgaccgaggc cgcgatgagt ttcgcgccga gctggtggcc ggcgtattcc
     1321 ggcaggcgcc cgaattcgag cacgtacccg ccgcagctgt ctttaaggaa gcaaagctct
     1381 ccaggctcgg tatcgtactc aatatcggtc aacccgatga gggcgccgtc ggccatggcc
     1441 accaggcacg tcgaggggcg cttgtacacg ggccgttgct ggatcgtgta attccacgcg
     1501 tgcgagatgc tcatgatgat cgcatgcgtg cgcatccatt gtgcttcgtc ttcgggccga
     1561 tattcacgga tctcgatatc cataccgtct gacgcctcct cccggccagt ccgagccatt
     1621 caccccgctc acgggcaccc tgaaacgcgg cacaagaccc gccagccgtg cggactgcct
     1681 gcaggcctca ttgtatccct ccaatggcca caggataccg cccagaccgt tgaagttcat
     1741 ctcttatcgg gcggcagatt gcgatcgccg ggctcaccaa tgccggagca cccatcgaaa
     1801 ctgtacgcag tgccggccta tccgagtctg tctcccgtgc gcg
//
LOCUS       DAOPBQ010000622         1843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_49942_length_1843_cov_3.308725, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000622 DAOPBQ010000000
VERSION     DAOPBQ010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49942_length_1843_cov_3.308725"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1792)
                     /locus_tag="PKW60_14840"
     CDS             complement(<1..1792)
                     /locus_tag="PKW60_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00761.1"
                     /translation="MTNRSSCVIGALAVFLCCAAWALADAGILPHGDAPPPVTFPHFP
                     DTAHAFVWRNWNAVEPAKIAEVLGASVEDITAMAVSMGLPAEPVVSPELKRRGYATLI
                     RRNWHLLPYEQLLQLIEMTPERLAFILREEDFLWIKLGQRKPACPPVRYQPPDAAASA
                     RAAEIRDLVHEAFGDEIALPAEPRFDFTRQIKDIPAGYTPPPAAATLSDPRRLVYSYV
                     AVFGDPLLTPELNPYPDGLLARLSEVGINGVWLQGLLRDLAPGGDAFPEFGEDHEIRL
                     ANLRELVQRAKTFGIGIYLYFNEPRALPNAFFEKHPEYAGVRENDLTALCTSHPAVRA
                     WMGDALAHIFREVPDLAGIYAITASENLTNCASHGQWSECERCKDRTDGDILAEVVQV
                     LEEGVHRSNPNAAVLISDWGWHGHGDAPDIIARLPKSVYLMSVSEWHLPIERGGIRST
                     VGEYSISAVGPGPRSLAEWKSAREAGLKTGTEIQFNNTCEIATVPYLPVMDLIAEHCV
                     NLREAAQLDAMLIGWTMGGFPSPNFQLAHRLSESPAPSAEAALDDVARERFGPDAAPG
                     VRRAWTLMSEAFREYPFYIGVVYTSPVQCGP"
BASE COUNT          352 a          557 c          606 g          328 t
ORIGIN      
        1 cggggccgca ctgcacaggc gaggtgtata ccacgccgat gtaaaacgga tattcgcgaa
       61 acgcctcgct catgagcgtc catgccctgc gcacgccggg cgcggcatcg gggccgaatc
      121 gttcacgagc gacatcatcc agggccgcct cagcgcttgg ggcgggcgac tcgctcaggc
      181 gatgcgcaag ctgaaagttg ggggagggaa acccgcccat ggtccagccg atcagcatgg
      241 cgtcgagctg ggccgcctcg cgcagattga cgcagtgttc ggcgatcaaa tccatgacgg
      301 ggagatacgg tacggtcgcg atttcacagg tgttgttgaa ttggatttcc gtgccggttt
      361 tcaggcctgc ttcacgggcc gacttccact cggccaggga gcgggggccc ggaccgaccg
      421 cagaaatgga atattcaccg accgttgacc ggatgccgcc acgttcgatg ggcaggtgcc
      481 attcgctgac ggacatcaga tacaccgatt tcggcaggcg cgcgatgatg tcgggggcgt
      541 cgccatggcc gtgccagccc caatcggaaa tcaggacagc ggcattcgga ttggagcggt
      601 gcacgccttc ttcaaggacc tgaaccactt ccgcgagaat atcgccgtcc gtgcggtctt
      661 tgcagcgttc gcattcgctc cattggccgt gagacgcgca gttggtcaag ttttccgacg
      721 ccgtgatcgc atagatcccg gccagatccg gcacttcgcg gaaaatgtgg gccagcgcgt
      781 cgcccatcca cgcgcgtacc gcaggatgag acgtgcacag ggccgtcaaa tcgttttccc
      841 gtacgccggc gtactcggga tgcttctcaa agaaggcatt gggaagagcc cgcggctcat
      901 tgaagtacag gtagatgccg atgccgaacg tcttggcgcg ctggaccagt tcccggaggt
      961 tggcgagccg tatctcgtgg tcttcgccga attcggggaa tgcgtcgccg cccggcgcca
     1021 aatctctcaa caggccctgc agccacacgc cattgatgcc gacttccgac aaacgcgcga
     1081 gcaaaccgtc cgggtaggga ttcagttcag gcgtgagcag cggatcgccg aagacggcca
     1141 cgtacgaata gaccagccgc cgcggatcgc tcaatgtggc cgccgcgggc ggcggcgtgt
     1201 agccggcggg gatgtctttg atctggcggg tgaaatcgaa gcgcggctcg gcgggaaggg
     1261 caatttcgtc gccgaaagcc tcgtgaacta agtcccggat ttccgcggcc ctcgcgctcg
     1321 ctgcggcgtc gggcggctgg taccgcacag gcgggcaggc cggcttgcgc tgcccgagct
     1381 tgatccacag aaaatcttcc tcacgcagga tgaatgcgag acgctccggg gtcatttcga
     1441 tgagttgaag caattgctca tacggcagga ggtgccagtt tctccggata agggtggcat
     1501 agccgcgcct ctttaattcg ggggacacca cgggctcggc aggcaatccc atcgaaacgg
     1561 ccatcgcggt gatgtcttcg acggatgcgc caagcacctc ggcgatcttt gccggttcga
     1621 cggcattcca gttgcgccac acgaacgcgt gcgcggtatc gggaaagtgc gggaacgtca
     1681 ccggcggcgg cgcgtcgccg tgcggaagta tcccagcatc tgcaagggcc caggccgcac
     1741 agcagaggaa aacggccaac gcgccgataa cacaggaaga tcgattggtc atgtctgagc
     1801 atccttgcgc tttgccgggc ccggtgcgcg cggccacggt ctg
//
LOCUS       DAOPBQ010000623         1806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_51446_length_1806_cov_3.486579, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000623 DAOPBQ010000000
VERSION     DAOPBQ010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51446_length_1806_cov_3.486579"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKW60_14845"
     CDS             complement(<1..530)
                     /locus_tag="PKW60_14845"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPK00762.1"
                     /translation="MSTSARLEQIIERAGDLPALPKAVSRVLALTDDPDMDLSEVADA
                     IQTDPALSAKILRISNSPYYGMRQYVGTLKLALVILGAREIRNIVLGVAVFDALRTRK
                     TESMLAGDFWKHSFETAAFSKKFGEYLKLTVHGEDFVTGLLHDIGKVVLMRQFEEEYA
                     TIYAETGGWGDALCKA"
     gene            complement(546..1379)
                     /locus_tag="PKW60_14850"
     CDS             complement(546..1379)
                     /locus_tag="PKW60_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPK00763.1"
                     /translation="MIKHILMPTDGSPAAEIGVQFAAAVATQYGATVRGLHVVDVRLL
                     EGPFLRDISASLGTAPYVNYQGNIAMILEERGKAALESVSRICANHGVECRTELVTGL
                     VVRSIVEQGELADLIILGRSGEHSKWLDGLVGSTTGGVVRRATRPVLVTGRETPGHGL
                     FVAAYDGSGYARAALRVAAGVTRDWGATLTVLVVGEGERAEGWANEAVAYLEPYQLPL
                     QAEVRKGDPSETIVAFAEETQADMLIMGAYGHTKVRRLVVGSTTAYAMNNSPCPLLLT
                     R"
     gene            complement(1376..>1806)
                     /locus_tag="PKW60_14855"
     CDS             complement(1376..>1806)
                     /locus_tag="PKW60_14855"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HPK00764.1"
                     /translation="ASGDLEIDAHHTVEDVGICLGKAFLQALGDFKGVRRFGHAVIPM
                     DETLAEAAIDFSGRPFFRFEGALPSGKAGDFDSELTEEFFRAFAVNSRSTLHLILRAG
                     ANLHHCIEGLFKAFARALDEAVQMDPRVQGVPSTKGMLET"
BASE COUNT          357 a          588 c          512 g          349 t
ORIGIN      
        1 tccgccttgc agagcgcgtc gccccagccg ccggtctccg cgtaaattgt ggcgtattcc
       61 tcctcgaact gacgcatcag caccactttg ccgatgtcgt gcaggagacc ggtcacaaaa
      121 tcttcaccat gcaccgtgag tttcagatat tcgccaaatt tcttcgagaa cgcagcggtt
      181 tcgaaagagt gtttccaaaa atcgcccgcg agcatggatt ccgttttccg cgttcgtaag
      241 gcatcgaaga cggccacgcc cagcacgata ttgcggattt cgcgcgcacc caggataacg
      301 agcgcaagct tcaaggttcc tacatactgc cgcatcccgt aatagggcga gttgctgatg
      361 cgcaggatct tggcgctcag cgcgggatct gtctggatcg catccgccac ctcagacaaa
      421 tccatatccg gatcgtcggt cagggccaat acacgtgaca ctgctttggg cagggccggc
      481 agatctccgg cccgctcgat aatctgctcg agtctggcgg atgttgacat ggctttccct
      541 ggaggttagc gggttaacaa taatggacag gggctgttgt tcatcgcata ggccgtggtg
      601 ctgccgacaa cgaggcggcg gactttcgta tgcccgtacg cgcccatgat cagcatatcg
      661 gcctgggttt cctctgcaaa tgctacgatg gtttcgctcg gatcgccttt tcgcacttcg
      721 gcctgcaggg gaagctggta gggctcgagg taggccacgg cctcgtttgc ccagccttcc
      781 gcacgctccc cttcccctac aacgagcacc gtgagcgtcg cgccccaatc ccgcgtcacc
      841 ccggcggcaa cacggagcgc ggcccgcgca tagcccgaac cgtcatacgc ggccacaaac
      901 aggccgtggc cgggcgtctc ccgtccggtc acgagaacgg gtcgcgtggc ccgccggaca
      961 acgcctcccg tggtggaacc caccaggccg tccagccatt tgctgtgctc cccgcttcgg
     1021 cccaggatga tgaggtcggc gagctcgccc tgctccacga tcgaacgcac caccagaccg
     1081 gtcaccagct ccgtgcggca ctcgacgcca tgattggcgc agattcgaga gacggattcc
     1141 agcgccgctt tgccgcgttc ctccagaatc atggcgatat ttccctggta attgacgtac
     1201 ggcgccgtcc cgagcgacgc ggaaatatcc cggagaaacg ggccctcaag cagtctgaca
     1261 tcgaccacgt gaagcccccg caccgtggcg ccgtattgcg tcgccacggc tgcggcgaac
     1321 tggacaccga tctcggctgc ggggcttcca tccgttggca tcaggatgtg cttgatcacg
     1381 tttccagcat acctttcgtg gatggcacgc cctggacacg cgggtccatc tgaaccgctt
     1441 cgtccagagc gcgcgcaaaa gctttgaaca gtccctcaat gcaatgatgc agattggcgc
     1501 cggcccgcag gataagatgc agggtcgagc ggctgttgac agcgaatgcg cggaaaaact
     1561 cctccgtcaa ttccgaatca aaatcgcccg ccttgcccga ggggagcgcg ccttcaaagc
     1621 ggaaaaaagg gcgcccgctg aaatcgattg ctgcttccgc aagcgtttcg tccatcggga
     1681 taacggcatg cccgaaacgg cggacccctt taaaatcgcc cagcgcctgc agaaaagcct
     1741 tccccaggca aatgcccaca tcttccacgg tatgatgcgc gtcgatttcc aggtctccgg
     1801 aggccg
//
LOCUS       DAOPBQ010000624         1803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_51584_length_1803_cov_3.730549, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000624 DAOPBQ010000000
VERSION     DAOPBQ010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51584_length_1803_cov_3.730549"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..940)
                     /locus_tag="PKW60_14860"
     CDS             complement(62..940)
                     /locus_tag="PKW60_14860"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPK00765.1"
                     /translation="MKIGRRDFFGHTAVAAAAAAIAPGAKAADSPAHAAPVPTETFSF
                     CVLADPHCAEPAKEGIEALGDGVAKFSRCLEQIQQLDPADRPDFVLAAGDIHPWALLE
                     REVNCTVPVYAVAGNHESSRERRQQLRQAFGRGFELNGKESDYYSFVHKGIRFVAACD
                     VGSGGDHIGHWCSEIITPSDQCEWLEQEFAQPEPVILFAHVPPERNGADRHMYLSRND
                     SRWFCETVEKQRPYAMFFGHLHHATEEYRIGETRCFNLCSCCWNADRTPLGFLHVRVT
                     PDGLHVREIETGRYSS"
     gene            complement(1016..1756)
                     /locus_tag="PKW60_14865"
     CDS             complement(1016..1756)
                     /locus_tag="PKW60_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HPK00766.1"
                     /translation="MPCLDMQNGRVVKGVHFVDIRDAGDPVECARAYCAAGADELALL
                     DITATVEGRATMLDVVRRTAEAISVPFTVGGGIADLAAAEAVLGAGADKVSISSAAFR
                     NPDLIRELVGAFGAGAVTVAIDVDRNPVLPSGYEVYIDGGRTATGADAIEWAKRIDGF
                     GVGCILPTSKAGDGAKTGYDLPVIRAMASAVNAALVASGGAGRLEHFYEAVEAGATVL
                     LAASVFHFHIVDIPELKLYLKERGVNVR"
BASE COUNT          367 a          559 c          532 g          345 t
ORIGIN      
        1 cagcgtttat tcagccggaa gctcgtgctc tgctgcctgc tttcagacta tgactttcaa
       61 tctaagagga atagcgcccg gtttctattt cacgaacgtg caggccgtca ggcgtcacgc
      121 gtacgtgcag gaatccaagc ggagtgcggt cggcattcca gcaacacgaa cacagattaa
      181 agcagcgggt ctcgccgatg cggtactcct cggtggcatg gtgcaggtgc ccgaaaaaca
      241 tcgcgtaggg cctctgcttt tccaccgtct cacagaacca gcgcgagtcg ttccggctga
      301 ggtacatgtg gcggtcagcg ccgttgcgtt ccggaggcac gtgcgcgaaa agaatcaccg
      361 gttcgggctg ggcgaactcc tgttcaagcc attcgcactg gtctgaaggc gtgatgatct
      421 ccgaacacca gtggccgatg tggtcgccgc cgctcccaac atcgcacgca gcgacaaacc
      481 ggataccttt gtgaacaaag ctgtaatagt cggattcctt gccgttcagt tcaaatcccc
      541 gaccaaacgc ctgccggagc tgttgtctcc gttcgcggga agactcgtga tttcccgcga
      601 cggcataaac cggcaccgtg caattaacct cgcgttcgag aagagcccag ggatggatat
      661 cgccggcggc caggacaaaa tccgggcggt cggccgggtc aagctgctgg atttgctcga
      721 ggcagcgcga gaatttggcc accccatccc ccagggcttc gatcccttcc tttgccggct
      781 ccgcgcaatg gggatcggca agcacgcaaa aagagaaagt ctcggtcgga accggcgccg
      841 catgtgccgg ggaatccgcg gctttcgcgc cgggtgcaat cgccgccgcc gcggcagcca
      901 cagcagtatg accaaagaaa tctctacgcc caatcttcat ggatctcact ccttttcagg
      961 cagttccggt gattttagcc gcagcagcac tcagttcgat gcgcagaatg gcatatcacc
     1021 ggacgtttac cccgcgctct ttcagataga gcttgagttc cggtatatcg acgatgtgga
     1081 agtggaatac ggatgccgca agcaataccg ttgcgcccgc ttcaactgcc tcatagaaat
     1141 gttccaaccg gcccgcgccg cccgacgcga caagcgcggc gtttacggcc gaggccatgg
     1201 cgcgaatgac ggggaggtcg tagccggttt ttgcgccgtc gccggccttg cttgtgggga
     1261 gaatgcagcc cacaccaaag ccatcaatcc gtttggccca ttcgatcgca tcggcgccgg
     1321 tcgcggtacg cccgccgtcg atatagacct cgtaccccga aggcaaaaca ggattccggt
     1381 caacatcaat tgccacggtt actgcgcccg cgccgaaggc gccgaccagc tcccggatta
     1441 aatccggatt gcggaatgcc gcactgctga tcgagacctt gtctgcaccc gcgccgagta
     1501 cagcttccgc ggctgcaaga tcggcgattc ctccgccgac ggtaaaggga acgctgatcg
     1561 cttcggcggt gcggcggacc acgtcgagca tggtggcgcg cccttccacg gtcgccgtga
     1621 tgtcgagcag agcgagttca tcggcgccgg cggcgcaata ggcgcgcgca cattcaacag
     1681 gatcgccggc atcgcggatg tccacaaagt ggacgccctt gaccacacgg ccgttctgca
     1741 tatccaggca gggcatgata ctgatggcag ctgtcacgag tgcctccgct ttgcacaggg
     1801 gtg
//
LOCUS       DAOPBQ010000625         1803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_51587_length_1803_cov_3.679062, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000625 DAOPBQ010000000
VERSION     DAOPBQ010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51587_length_1803_cov_3.679062"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PKW60_14870"
     CDS             <1..455
                     /locus_tag="PKW60_14870"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HPK00767.1"
                     /translation="CVDYLRSLEEVDPARIGCAGLSLGGEMAMWLGAMDPRVAATVSA
                     GFLTFMDQMEQNHCMCWKFEGLRELVDFPDIYAMIAPRPLQCQNGLQEPPTQFTPELA
                     RKALLEIVPAYRDTGAPGNLELLVHQGGHEIALESLVAFLKKHLNHAE"
     gene            714..914
                     /locus_tag="PKW60_14875"
     CDS             714..914
                     /locus_tag="PKW60_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPK00768.1"
                     /translation="MPTGTVKWFNEQKGYGFIIPDEGGKDIFVHRSNVATVNRTLKEN
                     EKVVYEVGDGRKGPEAKDVRTA"
     gene            1397..>1803
                     /locus_tag="PKW60_14880"
     CDS             1397..>1803
                     /locus_tag="PKW60_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00769.1"
                     /translation="MGERIAALAALSRIRSLVEQIPAQAVPSAEATPAIQEGAGVNAA
                     DAVTASRPLQELAGLRQMAEVTSQGTTENINARRVDRLGAALRRLMESTVDPPNAIQP
                     RQPVADALEVGADNHWSYNRAILEMLRGGFETAR"
BASE COUNT          391 a          507 c          558 g          347 t
ORIGIN      
        1 gctgcgtgga ctatctgcgc agccttgaag aggtcgaccc cgcccgcatc gggtgcgcgg
       61 gtctgtcgct gggcggggag atggccatgt ggctcggcgc gatggacccg cgagtggctg
      121 cgacggtgag cgcggggttc ctgacgttta tggaccagat ggagcagaac cactgcatgt
      181 gctggaagtt tgaagggctg cgcgaactgg tcgattttcc cgacatctac gccatgatcg
      241 cccccagacc gctgcaatgc cagaacggcc tccaggaacc tccgacgcag ttcacgccgg
      301 aactggcgcg taaagccctc cttgagattg tgcccgccta ccgcgatacc ggcgcgcccg
      361 gtaacctcga actccttgtg caccagggtg gtcacgaaat cgccctggaa tccctcgttg
      421 cattcctgaa aaagcacctg aatcacgctg aatgacggcc ttctcgctgc cgccgcctgc
      481 ccggcggccg gatttcccct tccaccgaca tgccctcctg aaacccgctt tggcactgaa
      541 aaactcgaat ttgagagtgc aaagagccga tttccgtcaa aagaggcccc aaatcgagct
      601 cataaaggcc atggattcca gaaaatccgt cattttcgct tgacagatgc tgtaatctgt
      661 gttcgaatgg ttaaagatga aggggccttt ggtttgcata cggagttacg actatgccta
      721 ccggtactgt gaagtggttc aatgagcaga aggggtacgg gttcatcatt ccagatgaag
      781 gcgggaagga catcttcgtt catcgctcca atgtcgcgac cgtcaatcgc acattgaaag
      841 agaacgaaaa ggttgtctac gaagtgggcg acgggcgcaa agggcctgag gcgaaagacg
      901 tccgtaccgc ctgagcctgg gcgacggggt tccggccctc ttaacggcgg ctgcaattca
      961 cagaccgggg cagggaaatc gtgcctgcgc gctgtaaaat acagctgcgc cacggcaaca
     1021 taaaacctga catttgtgct gaacgccacg actctgccca gcgcgggggg gtatcaaaaa
     1081 cgcagcaagg gcggagtgtg tccaaaactg cgagcctggg ccgggtagac tcgcgcattg
     1141 tttttcaagg gccgataatg aatagcgtgt gccggatcga cggcctggca gcgtttgaac
     1201 ccggatattc aggcactgtt ttccttcctg caataggttg tggataaacg atttgcggta
     1261 gatcgggcaa aaaaggcttc tattttggaa gcatttccga tatgctggtg ttgaggctgt
     1321 ggaaaggcgt atgcggtgcg ccttcccaag gtgcaacatt tctgacccga ccattccatc
     1381 tgcaggaatt gcgacaatgg gcgaacgcat agcagcattg gcggcgctga gccgtatccg
     1441 gtcgctggta gaacaaatcc cggcacaggc ggtcccatcg gcggaggcaa cgccagcgat
     1501 ccaggaaggg gccggcgtga atgcggctga tgccgttacg gcgtcgaggc cgctccagga
     1561 actcgccggg cttcggcaga tggccgaagt gacttcccag ggcaccacgg agaacattaa
     1621 cgcccggcgg gtggacaggc tgggcgctgc gctcagacgc ctcatggaat cgacggtgga
     1681 cccgccgaac gcgatccagc cccggcaacc ggtggcagac gcccttgagg tgggcgcaga
     1741 caatcattgg tcctacaacc gggcgattct ggagatgctc cgcggcggct tcgaaacggc
     1801 ccg
//
LOCUS       DAOPBQ010000626         1780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_52585_length_1780_cov_3.012754, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000626 DAOPBQ010000000
VERSION     DAOPBQ010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52585_length_1780_cov_3.012754"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKW60_14885"
     CDS             <1..393
                     /locus_tag="PKW60_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPK00770.1"
                     /translation="LVMNETLRKVARAHSQDMVDRDFFSHTSPDGGSLGDRLAGARVS
                     YVRAGENIAWNRGYANPVTVAVEGWMASPGHRANILRPEFALTGIGIAVAPDDAWYFT
                     QVFIDPSKSRGMDHVEIYTSGPIAITAP"
     gene            complement(422..1357)
                     /locus_tag="PKW60_14890"
     CDS             complement(422..1357)
                     /locus_tag="PKW60_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00771.1"
                     /translation="MHKHLEQMSDWLSTPSGRGLGAAGLLLLGIACLTMTQQVQYITG
                     YDALNGSAAVSIEDALQKNTYVFLAVSTVKAGLAVLEGSSVGVGFSIEIGDLVQSVYD
                     YVDYIWKVLLYGLTILAFYKMLLDTGFLAVGIKVIGVGLLVWAVGILRPAYRQTLHQI
                     GLRFAALGALVAYIVPLSLLATDAIGAMYIEPLKDKNREQMFAVMEQFDQARTEFSQL
                     REKVSLVNPGESLSQLKQGITRISSSLSHLLNNSLILFVQQTALLFFELLFFPFLSAF
                     VLYKMAQIGLSRLIERPGKRAATAADTSSARVDFA"
     gene            1526..>1780
                     /locus_tag="PKW60_14895"
     CDS             1526..>1780
                     /locus_tag="PKW60_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPK00772.1"
                     /translation="MADVRVRIAPSPTGFLHIGTAKMALFNWLFARKYNGTFILRLED
                     TDLERSAEEYAQAMCEGFHWLGIDWDEGPEFGGVPEKGAYG"
BASE COUNT          387 a          537 c          499 g          357 t
ORIGIN      
        1 ctggtcatga acgaaacgct ccggaaggtc gcgcgcgccc acagtcagga tatggttgac
       61 cgcgacttct tctctcacac cagccccgac ggcggttctc tcggtgaccg gttggcgggc
      121 gcccgcgtca gctacgtccg ggccggcgaa aatatcgcct ggaaccgggg ttacgccaat
      181 cccgtcaccg ttgcggtcga aggttggatg gcaagccccg gccaccgcgc caacatcctt
      241 cgccccgaat tcgccctcac cggcatcggt atcgccgtcg cgccggacga tgcctggtat
      301 ttcacgcagg tcttcatcga tcccagcaaa agccggggca tggaccacgt cgaaatctac
      361 acctccgggc cgatcgccat tacagctccc tgacacgggc tcgtttacag gatcggcacc
      421 ttcatgcaaa atccacccgc gccgacgaag tgtccgctgc ggttgccgca cgttttcccg
      481 gcctctcgat aagccgcgag aggccgatct gcgccatctt gtagagcacg aatgcgctga
      541 ggaaaggaaa gaagagcaat tcgaaaaaga gcagggcggt ctgctgtaca aacaggatca
      601 ggctgttatt gagcaggtgt gaaagcgagc tggaaattcg agtgatgccc tgtttcaact
      661 ggctcagact ctcgcccgga ttgaccagcg agaccttttc gcgcagctgg ctgaattctg
      721 tccgggcctg atcaaactgt tccataacgg cgaacatctg ttcgcggttc ttgtctttga
      781 gcggctcgat atacatggcg ccgattgcgt ctgttgccag aagcgatagc gggacaatat
      841 aggccaccag agcgccgagc gcggcgaatc gcaggccgat ctggtggagc gtctgtcgat
      901 acgcaggccg caaaatcccg acggcccaca cgagcagtcc cacaccgatg accttgatgc
      961 ccaccgcaag gaatcccgta tccagcagca tcttgtagaa ggccaggatg gtcagcccgt
     1021 ataacaagac cttccagatg taatcgacgt aatcgtaaac cgattgcacc agatcgccga
     1081 tctcgatgct gaatcccacg ccgaccgaac tgccttcgag gacggcgaga cccgctttga
     1141 ccgtagacac cgccaggaag acgtaggtat tcttctgcag cgcgtcttcg atggagaccg
     1201 ccgccgagcc gttcaacgca tcgtagccgg tgatatactg gacctgctgc gtcattgtca
     1261 ggcaggcgat cccgagcagg agcagaccgg ccgcgccaag cccccggccg gacggagtgc
     1321 tgagccagtc gctcatttgt tccaagtgct tatgcatagc ttttctccca agagagccac
     1381 ctttgcgcgc tccgggccgg aacgccacag cgtataattc acatttcagc gatttggttc
     1441 tttctcgtcg ccaaaaagat gcagagcact cctaaaatcg gtatcctgta tctcttgcaa
     1501 ctttgaaatg cccggggaga gttccatggc tgacgtacgt gttcgtattg ccccgtcacc
     1561 aacggggttc ctgcatatcg gcaccgccaa aatggcattg tttaactggt tattcgcgcg
     1621 taagtacaat ggtacattca tcttgcgcct cgaagacacc gacctcgaac gcagtgccga
     1681 agaatatgcc caggccatgt gcgaagggtt tcactggctg ggcatcgact gggacgaagg
     1741 gcccgagttc gggggagtgc ctgaaaaagg cgcctatggt
//
LOCUS       DAOPBQ010000627         1769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_53010_length_1769_cov_3.968495, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000627 DAOPBQ010000000
VERSION     DAOPBQ010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53010_length_1769_cov_3.968495"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PKW60_14900"
     CDS             <1..1024
                     /locus_tag="PKW60_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00773.1"
                     /translation="RIRLRMAALYALPMAFVAAGFMALRLMRMSAESTLFALDVTPGH
                     ALTVIRLATRYLLFPFHLSLRELVREHPWGALAAFAVAAGACWSLRRRAYAMPVAAGA
                     AWTVVTAALLLRTMSPWTLYLPSAGFCLALAGFAPAGCSRRECVPAAVMITLLFAYTL
                     QLEARKEAWWEADRVTRTCIDDFRHAVLDEERGDPILVCAPGAVADIPTLLHYFDMRM
                     RLETGRQDLDPLVLAFVMLPAEPQNTEILVKKEDAGMWRVQPADPGTTFLFPQKGYEF
                     QQYPANTAIKMPWGKATLRGANIAGEPTGLDIQVAPEAYVAWQGRKWYTFDGKHLVAL
                     PAQIAP"
     gene            1115..1483
                     /locus_tag="PKW60_14905"
     CDS             1115..1483
                     /locus_tag="PKW60_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00774.1"
                     /translation="MQHFICDGCSKEMKPDDTRYRVKIDVRVAKGSLEFSLLDLFRDH
                     RKELENLVKSFEGKDPRELEETIYKGFELDLCPRCQRAYIRDPLRFHPEQGLPDVDID
                     IDAFLRSLRQHSDNADETEE"
BASE COUNT          342 a          564 c          521 g          342 t
ORIGIN      
        1 gcgcatccgc ttgcgaatgg cagccctcta cgcattgccg atggcgttcg tcgcagccgg
       61 cttcatggcg ctgcgcttga tgcgcatgtc cgcggagagc acgctgttcg ccctcgatgt
      121 cacgcccggg cacgccctga cagtcattcg cctcgccacg cgctacctcc tgtttccatt
      181 ccatctgtca ctgcgggaac tggtccggga gcatccgtgg ggcgcactgg cagcctttgc
      241 cgtggcagcc ggcgcgtgct ggtcgctgag acgccgcgca tacgcaatgc ctgtggccgc
      301 aggtgcagcg tggaccgttg tcaccgcggc gctcctgctc cggactatga gcccatggac
      361 cctgtatctg ccctctgcag gattttgcct ggccctggca ggattcgctc cggccgggtg
      421 ttcccgccgg gaatgtgtcc cggcggccgt gatgataaca ctgctgtttg cctatacgct
      481 ccagctcgaa gcacgcaagg aagcctggtg ggaggccgat cgcgtaaccc gcacgtgcat
      541 tgatgatttc aggcatgccg tcctcgacga ggaacgaggc gatcccatcc tggtttgcgc
      601 tccgggcgcg gttgcggata tccccacgct gctgcactat tttgacatgc ggatgcgcct
      661 ggaaaccggg cgacaggatc ttgaccccct ggttctggcc ttcgtgatgc tcccggctga
      721 acctcaaaac acggaaatcc tcgtcaaaaa agaggacgcc ggaatgtggc gcgtgcaacc
      781 cgccgatccc ggcacgacgt ttctctttcc gcagaagggc tacgaattcc agcagtatcc
      841 agccaatacc gccatcaaaa tgccgtgggg caaagcgacc ctgcgcgggg ccaatatcgc
      901 cggagaacct accggactgg atattcaggt agctccagag gcgtacgtcg cctggcaggg
      961 gcgaaaatgg tatacgtttg acggcaaaca tctcgtggcg ctgcccgccc aaattgcccc
     1021 ctgactgcca gggccacagg cgcgtaccag gcagagccag ggctcgcctg ctataatcga
     1081 ctgtgacgca cccacgtaaa ccggagaggt gaaaatgcag cattttatat gcgatggatg
     1141 cagcaaagag atgaaaccgg acgacacgcg ctaccgggtc aaaatagacg tccgcgtcgc
     1201 aaaaggaagc ctggagttct ccctgctgga tttgttccgg gaccatcgga aagaactcga
     1261 aaacctggtg aaatcattcg aaggaaaaga cccccgagaa ctcgaagaga ctatttataa
     1321 ggggttcgag ctcgatttgt gcccgcgctg tcagcgcgcc tacatccgtg accccttgcg
     1381 attccatcca gaacagggcc tgcccgacgt agacatcgat atcgacgcct tcctgcgttc
     1441 actgcgccag cactcggaca acgcggacga aacggaggaa taacctttac ggcaaggccg
     1501 gtatttggct ctcattccat gacgtgagcg cgccaggcag cacacgggat ccggccatct
     1561 cgatccaggg cgaacgccgt ctcgcgccct tcagtcttgc tcttcatcgt gccggatggg
     1621 tgctccgtca tcttccgccg ccgagtcgct ggtgccggcc tcgtccgcgg cccgtccctc
     1681 cgggctttcg ttgctcttgc cgttgacgcc ggagtggccg tcgcgcaggg ccacaatggc
     1741 ccacaacgcc agcgttgagc agagaagtc
//
LOCUS       DAOPBQ010000628         1737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_54446_length_1737_cov_3.177765, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000628 DAOPBQ010000000
VERSION     DAOPBQ010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54446_length_1737_cov_3.177765"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKW60_14910"
     CDS             complement(<1..582)
                     /locus_tag="PKW60_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPK00775.1"
                     /translation="MSILTLLGLDKTFSHGVHPPEYKALTEHKPIRRLPFADEMIIPL
                     SQHTGAPAQPIVREGQEVVRSEPIAKPGGFVSVPMHAPATGRITRIALARDVRGGMSP
                     AIFLRLYPGEGQEILYGAPIDVDGMSNPEIVKAVQWTGVVGLGGAAFPTHVKLAVPEG
                     KKVDTIIVNGCECEPYLTTDHRLMVEHPEDVYEG"
     gene            complement(674..>1737)
                     /locus_tag="PKW60_14915"
     CDS             complement(674..>1737)
                     /locus_tag="PKW60_14915"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPK00776.1"
                     /translation="GTIANVGHLLEGFVQGLTARRPAFFSIHSPCMPEHGIGDDVAKH
                     QSILAVESRAYPVFRYNPDLGENPADALDLEGNPALNSDWPTFTLSYVDEEGQPGTME
                     IPLTFADYAATESRFRKHYRKAPQDAWNDDMVPIADFINLSEEDREGLYPYVWAVDKK
                     NRLSRLICSKSIVKSTEDRRNFWNLLKSLTGADQKVDVNQIANQARAEVVQKITAGLL
                     QLANGGSVSHAVPAPAAMAVVAPATGGGGAAVEQNVAVAPAPDAPGLVTGGGMDPYLD
                     SEQCTGCGECQLINPTMFGWNDKKQAIVLDYKSGPYKDMVKAAERCAARCIRPGDPWD
                     PNEKGLDKLVKRAAKFNEM"
BASE COUNT          297 a          539 c          541 g          360 t
ORIGIN      
        1 gccttcatag acatcctcag ggtgctcgac catcaggcgg tggtcggtgg tgaggtacgg
       61 ctcgcattcg cagccgttca cgatgatcgt gtcgactttc ttcccttccg gcacggcgag
      121 tttgacgtgc gtggggaatg ccgcgccgcc caggcccact acgcctgtcc actgcacggc
      181 tttgacgatc tccgggttgg acatgccgtc gacatcgatc ggcgcgccat acaggatttc
      241 ctggccctcg ccgggataca gccgaaggaa gatggcgggg gacatgccgc cccgcacgtc
      301 gcgcgcaagg gcgatacggg tgatccggcc ggtagcgggc gcatgcatgg gcaccgacac
      361 aaatccgccc ggcttggcga tcggctcgct tcgcacgact tcctgacctt cgcgaacgat
      421 gggctgggct ggcgcgccgg tgtgttgcga cagcggaatg atcatctcgt ccgcaaaggg
      481 cagacggcga atgggcttat gctccgtcag cgctttgtac tcggggggat gcaccccatg
      541 cgagaacgtc ttgtcgaggc cgagtaatgt caatatgctc atggatacca gcgcgtgtta
      601 tgtgggcgcg tcccatgacc cccagggacg cgcccacccg tttctgatcg agcgggcgcg
      661 ttgcccgcga tggctacatc tcgttgaatt tagcagctcg ttttacgagc ttgtcgaggc
      721 ctttttcatt cggatcccag ggatcgccgg ggcggatgca ccgggccgcg caacgttccg
      781 cggctttgac catgtccttg taggggcccg atttgtagtc aagcacgatc gcctgttttt
      841 tgtcgttcca gccaaacata gtcgggttga tgagctggca ctcaccacaa cccgtgcact
      901 gctccgaatc aagatacgga tccattccac ctccagtaac cagccccggg gcgtccggtg
      961 cgggggcaac agccacgttc tgctctactg cggctccgcc gccgccggtt gctggtgcaa
     1021 ccacggccat ggcggccggg gccggcacgg cgtgcgaaac gctgccgccg ttggccaatt
     1081 ggagcaggcc ggccgtgatc ttctgcacca cttccgcacg cgcctggttg gcaatttggt
     1141 ttacgtcgac tttctgatca gcgcccgtga gggacttgag caagttccag aaattgcgcc
     1201 ggtcttcggt cgacttcaca atcgatttcg aacaaatcag gcgggacaaa cggttctttt
     1261 tgtcgaccgc ccacacatac ggatacagtc cctcgcggtc ttcctcgctc agattgatga
     1321 agtcggcgat gggcaccatg tcatcgttcc aggcatcctg cggagccttg cggtaatgct
     1381 tgcggaaacg gctttccgtg gccgcgtagt ccgcgaaggt cagcgggatc tccatcgtgc
     1441 ccggctggcc ttcttcatcg acatagctga gcgtgaaggt cggccaatcg ctgttcagcg
     1501 cggggttgcc ttccaggtcg agcgcgtccg ccggattctc accgagatcc gggttgtacc
     1561 ggaacacggg gtacgcacgc gattcaaccg ccaggatcga ctggtgcttg gccacgtcat
     1621 cgccgatgcc gtgctcaggc atgcaggggc tgtgaatcga gaagaaggcg ggcctccgcg
     1681 cggtcagacc ctgcacaaag ccttccagca ggtggccgac gttggcgatc gtcccct
//
LOCUS       DAOPBQ010000629         1736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_54482_length_1736_cov_4.008923, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000629 DAOPBQ010000000
VERSION     DAOPBQ010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54482_length_1736_cov_4.008923"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PKW60_14920"
     CDS             complement(<1..696)
                     /locus_tag="PKW60_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPK00777.1"
                     /translation="MAAVFAVAAFAAFAQDAKYEPTWESVESRPLPGWFDHDKFGIFI
                     HWGLYSVPSWGPKGRYSEWYWHDMQDKNGETWKFHVENYGEDFKYPDFAPQFRAEMFN
                     PAHWAEIFERSGAKYVVLTSKHHEGFCLWPNAESWNWNSVDAGPGRDLCGELTDAVKA
                     RGLKMGFYFSLYEWFNPIYRSDPERYVEQHMLPQFKDLVMRYEPDIVWPDGEWDHPDT
                     LWRSAEFIAWLYNE"
     gene            complement(777..>1736)
                     /locus_tag="PKW60_14925"
     CDS             complement(777..>1736)
                     /locus_tag="PKW60_14925"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPK00778.1"
                     /translation="IDVQMRQVDDIDGIECHYLTFLSESDDRVPAYLLIPKGARPGPA
                     PALICPHPTSSGAGKSQCVGMTGRAPGEPPLPPEKSRAYGLELARWGYVTLSIDLFCD
                     GERIPPGHTHYDSEPFYKRHPEWSMFGKNAWDCMRAVDALETLDFVDRDRIGCIGHSL
                     GGHTTLCAAAFDERIAAAVSNCGEVGWERGTDHWSRPQDPNKKRGPVSDYCYLPNFAP
                     YVKEPTRPVPCDFDGLMMLVAPRPLLIQGTEDEFKRLGTVEQVISAQAAYHTLGAGER
                     VGMISFPGEHNFSPGAKRFAFAWLDRWLGHTPAVPTIWPGLVL"
BASE COUNT          331 a          584 c          495 g          326 t
ORIGIN      
        1 ttcgttatac agccacgcga taaactccgc actgcgccag agggtgtccg ggtggtccca
       61 ctcgccgtcg ggccacacaa tatccggctc gtagcgcatg acaaggtcct tgaactgcgg
      121 cagcatgtgc tgctccacgt accgttcggg atcgctgcgg taaatcgggt tgaaccactc
      181 gtacagcgag aagtagaacc ccattttcag tccgcgcgcc ttcaccgcgt ccgtcagctc
      241 gccgcagagg tcgcgcccgg ggcccgcgtc cacgctgttc cagttccagc tctccgcgtt
      301 gggccacaga cagaacccct cgtggtgttt cgaggtcagc acaacgtatt tcgcgcccga
      361 tcgctcgaag atctccgccc aatgcgcggg attgaacatt tccgcgcgga attgcggcgc
      421 aaaatcgggg tatttaaagt cttccccgta attttccaca tgaaatttcc aagtctcgcc
      481 gtttttgtcc tgcatgtcat gccagtacca ctccgaatac cggcctttcg ggccccagga
      541 gggcaccgaa tacagccccc agtggatgaa gatgccgaat ttgtcgtggt cgaaccagcc
      601 cggcagaggg cggctttcaa ccgattccca ggtgggctcg tatttcgcgt cctgggcgaa
      661 tgcggcgaac gccgccacgg caaacactgc cgccatcaca catcttgccg ttcgcatcgt
      721 ttgtgtccct ccactctgcg cgggcccacc gcgctgtcgt tcccatccaa atggcgttac
      781 agcaccagac cgggccagat cgtaggcacg gcgggcgtat ggcccagcca gcgatccagc
      841 cacgcgaacg caaaacgctt cgccccgggc gaaaaattgt gctcgcccgg aaatgaaatc
      901 atccccacgc gctcaccagc accgagggta tggtaggccg cctgagcgct gatcacctgc
      961 tcgacggtgc caagccgttt gaattcgtcc tccgttccct gaatgagaag cggacgcggc
     1021 gccaccagca tcatcaaacc gtcaaaatca cacggaaccg gccgcgtggg ttctttaaca
     1081 tacggcgcga aattcggcag ataacagtaa tcgctcaccg gcccgcgctt cttattcggg
     1141 tcctggggcc gcgaccagtg gtcggtgccc cgttcccatc ccacttcgcc gcagttgctc
     1201 acggccgcgg caatgcgctc atcgaaggcc gccgcgcaca aggtcgtgtg gccgcccagc
     1261 gaatggccga tgcacccgat ccggtcccgg tccacaaagt ccagcgtctc cagcgcatcc
     1321 accgcgcgca tacagtccca cgcgttcttg ccgaacatgg accactcggg gtggcgtttg
     1381 tagaacggct cggaatcgta atgcgtatgc cccggcggaa tgcgctcgcc gtcgcagaac
     1441 aggtcgatgc tgagggtcac gtagccccag cgggcgagtt ccaggccata ggcgcggctc
     1501 ttttcgggcg gcagcggcgg ctctccgggc gcacggcctg tcattccgac gcattgcgac
     1561 tttccagcgc ctgagcttgt cggatgaggg cagatcagcg ccggcgccgg gccgggacgc
     1621 gcccccttcg gaatcagcag gtacgcgggc acgcgatcat ccgactccga caaaaacgtc
     1681 aggtaatggc actcgatccc gtcgatatcg tctacctggc gcatctgaac gtcgat
//
LOCUS       DAOPBQ010000630         1721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_55190_length_1721_cov_3.311525, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000630 DAOPBQ010000000
VERSION     DAOPBQ010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55190_length_1721_cov_3.311525"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1721)
                     /locus_tag="PKW60_14930"
     CDS             complement(<1..>1721)
                     /locus_tag="PKW60_14930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPK00779.1"
                     /translation="LSYYSVRAFSLASKSVQNALAFAAVCAVAVPFCYTLRRVHRSSR
                     VTAVAVAAAILLVFPPMLSLVQTAMFAPLSSLPLIRIFFENSKHFANIALNIGFLTFF
                     VAFYLSMMETEKDKLLIEEQMQEVQERDDAYRTLVENTLQALVIWQDRRFLFVNSVFE
                     QISGYSASELVAMTAEQVRELIHPDDRDWVWERGARRLAGGDEPAHYEFRIICKDGTI
                     KWFEVFTAFTQFRGEPVIQQSGIDVTERRAAEEALRNSELKYRTMAESISDIVWTTDM
                     ENRFTYVSPSVEHILGYTVEEALASKSTKVLVPESEARLKTQAKAAIERAAQGGDVWV
                     TQTPQEYHHFHKQGHVVPCEVTCKLLLDEHGIPTGLIGVTRDITERKRIEESLRASEE
                     KYRTMAESISDVVWMTDIEGYVSYCNRATERVLGYTPGELAGVHMRQILTESAFDRAR
                     ATIRESLDQLSQGERSNDPPQEYDHVHKDGTIVPCEVVSRLIFDETGAPVAMVGVSRD
                     ARERKRADAERRRLEARMQQAQKLESMGILAGGIAHDFNNILVGILGNADLALEELSV
                     GSPSRAY"
BASE COUNT          332 a          497 c          505 g          387 t
ORIGIN      
        1 gataggcgcg cgacggggag ccaaccgaca gctcttcgag tgcgaggtcg gcatttccca
       61 ggatgcccac caggatatta ttgaaatcat gggcgatccc cccggccagg atgcccatgc
      121 tttccagctt ctgggcttgt tgcatccggg cctcgaggcg gcgcctttcc gcgtcggcac
      181 gtttgcgttc gcgagcgtcg cggcttacgc ccaccatggc aacgggcgcg cccgtctcat
      241 caaaaatcag ccggctgacc acttcacagg ggacgatggt cccgtctttg tgcacatgat
      301 cgtattcctg aggcgggtcg ttgctgcgtt ctccctgcga gagctggtcg agagattccc
      361 ggatggtggc tcgggcacga tcaaaggcgg actcggttag aatctgacgc atatgcaccc
      421 ccgccagctc gcccggcgtg tagcccaaaa cccgttccgt ggcccggttg cagtaagaca
      481 cgtatccttc gatatccgtc atccagacta cgtcggaaat gctctccgcc atagtccggt
      541 atttttcttc gctggcccgc aggctttctt caatgcgttt gcgctcggtg atatcgcgcg
      601 tcactccaat gaggccagtg ggtatcccat gctcgtccag tagaagcttg caggtcactt
      661 cgcacggaac gacatggccc tgtttgtgaa aatgatggta ttcctgcggg gtttgggtca
      721 cccagacgtc gccgccctgg gcggcgcgtt caatggctgc tttggcctgc gttttgagac
      781 gggcctcgga ttcaggcacc agcactttgg ttgatttgga ggcgagtgcc tcctcgaccg
      841 tgtagcccag gatgtgttca acggatgggc tcacgtaggt gaaccggttt tccatatccg
      901 tagtccagac gatatcggag atgctctcgg ccatggtgcg gtatttcagt tcgctgttcc
      961 gaagggcttc ttccgcagcg cgcctttcgg tgacgtcaat accggattgc tggataacgg
     1021 gttcgccacg aaactgagta aacgcggtaa atacttcaaa ccatttgatc gtgccgtctt
     1081 tgcagattat ccggaattca taatgagccg gttcatcgcc gcctgcgaga cggcgcgcgc
     1141 cgcgttccca cacccaatcc cgatcgtccg ggtggatcag ttcccgcacc tgttcggcgg
     1201 tcatggccac cagttcgctg gcgctgtagc cgctgatttg ctcgaaaacc gagttcacga
     1261 acaaaaaccg gcgatcctgc cagatgacga gggcttgcag tgtgttttca accagggtcc
     1321 ggtacgcgtc atcacgctcc tggacctcct gcatttgttc ttcgatcagc agtttgtctt
     1381 tttcggtttc catcatgctg agatagaagg ccacgaagaa cgtaaggaac ccgatgttca
     1441 gggcaatgtt ggcgaaatgc ttgctgttct cgaaaaagat gcgtatcagc ggcagagagc
     1501 tcagcggcgc gaacatggcc gtctgaacca ggctgagcat cggcgggaag accagcaaga
     1561 tcgctgcggc cactgccaca gcggtcaccc gtgaggaccg gtgcacgcgc cggagggtgt
     1621 agcagaacgg gactgccacc gcacataccg cggcgaatgc cagggcgttc tgtactgatt
     1681 tcgatgccag gctgaaggca cgaactgaat aatagctgag t
//
LOCUS       DAOPBQ010000631         1702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_56150_length_1702_cov_3.744384, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000631 DAOPBQ010000000
VERSION     DAOPBQ010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56150_length_1702_cov_3.744384"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PKW60_14935"
     CDS             complement(<1..1074)
                     /locus_tag="PKW60_14935"
                     /inference="COORDINATES: protein motif:HMM:NF036726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HPK00780.1"
                     /translation="MQLADVFEQIQESDWQGEVALHWDDSMACMPDVLPFLEPDQIAE
                     NLDWGGLESSHQQALEEAARQIAGTPELKALAWHAWRRLYESDDPVDFNHWPALDAAM
                     NDTSGCFYLLLGLAMVPGTRAIHESLGIPSSVTRETCLQVACFADNFRRARHGRLGMF
                     RGQLFWMRNYPQGRLFRLGRFEYRLQPLGPFVHVFRHREQNLTVVLSAGGRLFDDAGY
                     AVQIGTAGSWEATFTLSGEAARGFPVHPAGHVLRYEVTLPRSEWDHVVKPGDTMLDVH
                     IPSGGQMTPQSCLDTMRRSVEFFRTYFPDKPFRSFWCHSWIFGPQLETILPETANLVK
                     YLREVYLYPVYSEDGGLWFIFFQD"
     gene            1456..1701
                     /locus_tag="PKW60_14940"
     CDS             1456..1701
                     /locus_tag="PKW60_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPK00781.1"
                     /translation="MTEINVAETVKKIVVENLERKPEEVTDDARFIEDLGADSLDLTE
                     LLMALEEEYNVEIDDEANDIQTVGDAIKYIESKLNNQ"
BASE COUNT          375 a          528 c          499 g          300 t
ORIGIN      
        1 gtcctgaaaa aagataaacc ataatccgcc gtcttccgag tagacgggat acaggtacac
       61 ctcacgcaaa tatttcacaa gattggcggt ttccggcagg atcgtctcca gttgaggccc
      121 gaagatccac gaatggcacc agaagctccg gaacggcttg tccgggaaat acgtccggaa
      181 aaactcgacg ctccggcgca tggtgtcgag gcaactctgg ggcgtcatct ggccgccgga
      241 tgggatgtgg acatccagca tagtgtcgcc aggcttcacc acgtggtccc attccgagcg
      301 gggcagggtc acttcatagc gcaggacgtg ccccgcggga tggacgggga atccgcgcgc
      361 ggcctcgccc gacagcgtga aggtggcctc ccagcttccg gcggtaccga tctgtacggc
      421 gtagccggcg tcatcaaaca accgcccgcc ggcggacagg accaccgtga ggttctgttc
      481 gcgatgccgg aacacatgca cgaaaggtcc caggggctgg aggcggtatt cgaaacgccc
      541 cagccgaaac agccggccct gcgggtaatt gcgcatccag aacagctggc cgcggaacat
      601 tcccagccgg ccgtgccgcg cccggcggaa attgtccgca aaacatgcca cctgcaggca
      661 ggtttcgcga gtgacggaac tgggtatgcc gagcgattcg tggatggcgc gggtgcccgg
      721 caccattgcc aggcccagca gcaggtagaa gcatccgctc gtatcgttca tcgcagcgtc
      781 gagcgccggc cagtgattga aatcaacggg gtcgtccgac tcgtagagac ggcgccaggc
      841 gtgccacgcc agggctttca actcgggcgt cccagcgatc tgccgggcgg cctcttcgag
      901 cgcttgctga tgcgagctct ccagcccgcc ccaatccagg ttttcagcga tctggtccgg
      961 ttccaggaac ggcagcacgt ccggcatgca cgccatggaa tcgtcccaat gcagcgccac
     1021 ctcaccctgc cagtcacttt cctgaatctg ttcgaatacg tccgccagct gcatccgttt
     1081 tacctcccgg cacggtcgca cgggcatccc acacactccg caccaatcac ctgagtcaca
     1141 ttggaatccg ttttatggaa aaagttgcgt gattgaaccc gcgctggagt ggtgccggcg
     1201 caatgggcga tggcatacct gatgcctccc gccgcgcgcg ctttctccag agggccccat
     1261 tcacgcgact ctcccgccac aatcctactc aagcacgtcg ccgggcgcaa ccgttctgcc
     1321 aaagcgtttg aacaccgaaa actagaaatt cgccctggcc gatggtatac tctgccctta
     1381 tccgacccag ggggaatctg tgactgggtg gcagtgagtt cagcaagccc caactatgga
     1441 agggaggtgt tgcggatgac agagatcaat gtcgcggaaa ccgtcaagaa gatcgtcgtg
     1501 gaaaacctcg aacggaaacc cgaggaagta accgacgacg ctcgtttcat cgaagatctg
     1561 ggcgcggatt cgcttgatct caccgagctg ctcatggcgc tcgaggagga gtacaacgtc
     1621 gagatcgacg acgaagcgaa cgacatccag actgtcggtg acgcgatcaa atacatcgaa
     1681 tcgaagctca acaatcaata ga
//
LOCUS       DAOPBQ010000632         1696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_56442_length_1696_cov_3.020110, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000632 DAOPBQ010000000
VERSION     DAOPBQ010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56442_length_1696_cov_3.020110"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKW60_14945"
     CDS             <1..391
                     /locus_tag="PKW60_14945"
                     /inference="COORDINATES: protein motif:HMM:NF028226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HPK00782.1"
                     /translation="IYLDPVAAIVVSVLLLHAAGKITWPVLHQLVDAGASEAEVAAMR
                     QIIETTEGVKSIHELRTRHIGSGLQLDVHVLVAPALTVREGHAIATAVKQRLLNEKEE
                     VVDVLVHIEPFEAPYWRDAGTEQSPES"
     gene            complement(425..1639)
                     /locus_tag="PKW60_14950"
     CDS             complement(425..1639)
                     /locus_tag="PKW60_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPK00783.1"
                     /translation="MLRFGCLMILVLVSVKAGLAGAAPLSADDFTFEGPLGSQGTTIE
                     KVGENHFVVSLGAAPGHPDWNNRPQFTILRNAKGNSLRLDVQFDGGPAYYFNEYAFSY
                     SYDGEHWQPVHWLNGPSVDKQHDTLVFPHFEQDVVYVGHQVPMSYEKLVELVAAWKKS
                     PFVTVHVLGKSIEGRDLYRLEITDPKSDVPRKKRWAHFFANQHPGEHNSQWRMAGMID
                     WMLSGEAAGFRRRSICHFVPMMSPDAPSKGWYRVNAQGYDMNRTYLHTGADQASQAHE
                     ACIVQRDLEMLMASEAPPVSAWSIHTWGGKTDPRIIAGPEMTEDKLGPWTRFGDIIEQ
                     YDVNDVIRPMEEWPLRDGHQNQWTEGTHTQFGITSILCEGGGALYTKEENVEAGEVLM
                     RAIADYYAGTRD"
BASE COUNT          317 a          486 c          526 g          367 t
ORIGIN      
        1 gatctatctc gatcctgtcg ccgcgattgt ggtgtcggtg ctgcttctgc atgccgcggg
       61 gaagatcacg tggccggtat tgcatcaatt ggtcgacgca ggcgcgagcg aggccgaggt
      121 ggcggcgatg cgccagatca tcgagacaac cgaaggcgtc aagtcaatac atgaactgag
      181 gacccgccac atcgggtcag ggctgcaact cgatgtgcat gtgcttgtag ctcccgcctt
      241 gaccgttcgc gaaggtcacg ccattgcaac cgccgtgaag cagcgcctcc tgaacgaaaa
      301 ggaggaggtg gtcgatgttc tcgtgcacat cgagccgttt gaagcgccgt attggcgcga
      361 tgccgggaca gaacaatcgc cggaaagttg agtgctccat tcggcataaa gggcgggttt
      421 gcgcttaatc ccgggtgcct gcgtaataat ccgcgatggc tctcatgagt acctcgcccg
      481 cctcgacgtt ttcctctttg gtgtagagcg cgccgcctcc ttcacagagg atggaggtga
      541 tgccgaactg ggtgtgcgtg ccttcggtcc actgattctg gtggccgtca cgcagcggcc
      601 attcttccat cgggcgaatc acatcgttca catcatactg ttcgatgatg tcgccgaagc
      661 gagtccaggg ccccagcttg tcctcggtca tttccggtcc cgcgatgatc cggggatcgg
      721 tcttcccgcc ccaggtgtgg atgctccagg cgctgactgg gggtgcttcg gacgccataa
      781 gcatttcgag atcacgctgc acaatgcagg cttcgtgggc ctggctggcc tggtccgcgc
      841 ctgtgtgaag atacgtgcgg ttcatgtcat acccctgcgc attgacgcgg taccagccct
      901 tggagggcgc gtcggggctc atcatgggca cgaaatggca gatgctgcgg cggcggaagc
      961 cggctgcctc gccactaagc atccagtcga tcatgcccgc catgcgccat tgcgaattat
     1021 gctcgcccgg atgctggttg gcgaagaagt gggcccagcg ttttttgcgg ggcacgtcgc
     1081 ttttgggatc ggtgatttcg aggcggtaga gatcgcggcc ttcgatggat ttcccgagga
     1141 cgtgtaccgt cacaaaaggg cttttcttcc aggccgcgac gagttccacg agtttctcgt
     1201 atgacatggg cacctggtgc ccgacgtaaa ccacgtcctg ttcaaagtgc gggaagacca
     1261 gggtatcgtg ctgcttgtca acgctcgggc cgttcagcca gtgcacgggc tgccaatgtt
     1321 cgccgtcgta ggaatacgaa aaggcgtatt cattgaagta atatgccggc ccgccgtcaa
     1381 attggacatc gagccgcaga gaattgcctt ttgcgttgcg caggatggtg aattgcgggc
     1441 ggttgttcca gtccggatgg ccgggcgcgg cgcccagcga taccacgaaa tggttctccc
     1501 caactttttc gatggttgtg ccctgcgacc ccagtggccc ttcgaatgtg aaatcgtctg
     1561 cggatagcgg ggctgcgcca gccaggccgg ccttcaccga gaccagcaca agaatcatca
     1621 agcatccgaa tcgcaacatg gcacctccat taccttgcac agacactacg gcgcgagcac
     1681 gtctgcatgg gtatcg
//
LOCUS       DAOPBQ010000633         1680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_57308_length_1680_cov_2.574769, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000633 DAOPBQ010000000
VERSION     DAOPBQ010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57308_length_1680_cov_2.574769"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1371
                     /locus_tag="PKW60_14955"
     CDS             94..1371
                     /locus_tag="PKW60_14955"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HPK00784.1"
                     /translation="MKKKATKARKRATRRPAGTSYTTLRQIVQWIPAGLPDRIARDAG
                     ADIRKFSCTSHVLALLHGHIVRAGSLNEICDAARLHEPELNRIRGATAPKRNTFSNAN
                     RRRDPKIAEDFYWEMFEHLMTICPSFTQYKKHSGFIFRLKRGIFAIDSTTLKLTPDSI
                     DRARHRRKKTAAKTHMAIDVGSRLPTFAVVEDAAHHDSTRADVLCAGLKDGDVLLADR
                     AYVDFRFLYGLAERGVFLVLREKENMDFGVLEERGHQDRRILKDQTIRLRGKLTAEKY
                     PEPLRRVTAVVEVDGQEREMTFITNNFAWSPRTVSELYRARWVIESFFKELKQTLQLT
                     DFVGYNENAVKWQVWIGLLAHLLLRFLKHVSKWGLSFSRLAGVVRAAVWMRIDLLDAL
                     RKYGTAGGPKRPVIVGKQLYFQGFEPFASWPVG"
BASE COUNT          352 a          523 c          515 g          290 t
ORIGIN      
        1 cggaggccag cgcacgcgct caagacgaga gcgccagccc ggattgaaaa aaaggctgac
       61 ctcccggccg tctgcctgta gcctacaggc attatgaaaa agaaagcgac caaggcgcgt
      121 aaacgcgcaa ccaggaggcc agcgggaaca tcgtacacca ccctgcgcca gatcgtccag
      181 tggattcccg cgggattgcc ggacaggatc gcgcgcgacg ccggggcgga catccgtaag
      241 ttctcctgca ccagccatgt gctggcgctg ctccacggcc atatcgtccg cgctggcagc
      301 ctgaacgaga tctgcgacgc ggcccggctg cacgagccgg agctgaaccg catccggggc
      361 gcgacggccc cgaagcgcaa caccttctcc aacgccaacc ggaggcgcga cccgaagatc
      421 gcggaggatt tctactggga gatgttcgag cacctcatga ccatctgccc gtccttcacg
      481 cagtacaaga agcacagtgg cttcatcttc cgcctgaagc gcgggatatt cgccatcgac
      541 tcgaccacgc tgaaactcac gcccgactcg atcgaccggg ccaggcaccg ccggaagaag
      601 accgccgcca agacccacat ggccatcgac gtgggcagca ggcttccgac gttcgcggtc
      661 gtggaggatg ccgcgcacca cgactcgacg cgggctgacg tgctgtgcgc gggactgaag
      721 gacggcgacg tgctgctggc ggaccgcgcc tacgtcgact tccgcttcct gtacggactc
      781 gccgagcgcg gcgtcttcct cgtcctgcgg gagaaggaga acatggactt cggggtgctg
      841 gaggagagag ggcaccagga ccggcgcata ctcaaggacc agaccatacg cctgcgtggc
      901 aagctgacgg cggaaaagta ccccgagccc ctgcgccgcg tcaccgctgt cgtcgaggtg
      961 gacggacagg agcgggaaat gacgttcatc accaacaatt tcgcgtggag cccccgcacc
     1021 gtctccgaac tctaccgcgc ccggtgggtc atcgagtcgt tcttcaagga actcaagcag
     1081 acgctccagc tcacggattt cgtcgggtac aatgagaacg cggtcaagtg gcaggtctgg
     1141 atcgggctgc tggcgcatct gctgctgcgc ttcctcaagc acgtctcgaa atggggactg
     1201 agcttttccc ggctcgccgg ggtcgtcagg gccgccgtct ggatgaggat cgacctgctc
     1261 gacgccctgc ggaagtatgg gacagcaggc ggccccaagc gccctgtaat tgtcgggaaa
     1321 cagctctatt tccaaggatt tgagccattc gcgagctggc ctgtgggata gcacatgccc
     1381 gaaaaccctt gttttcgggc atccgggccg aatgcgccgg caaacacgtt ttcgggaacg
     1441 cctttgaaat cgcgataaaa ccctgcaaat caatggggga aagggggcag tgtacccgtt
     1501 ttcagccgcc tatgggatgc tagtgccttt cattaaatca tgcaatgttg tttgcggaac
     1561 tatttcctgc ggtctggcgt atcgggtagt gcggaacaaa cagctgcttc ccgtattcat
     1621 gtccgggctc aaatacaggc tggccatgcc acccgcatca gaaatgactc gttggaatac
//
LOCUS       DAOPBQ010000634         1671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_57746_length_1671_cov_4.255569, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000634 DAOPBQ010000000
VERSION     DAOPBQ010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57746_length_1671_cov_4.255569"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="PKW60_14960"
     CDS             <1..1177
                     /locus_tag="PKW60_14960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPK00785.1"
                     /translation="IGVTIQLDENGNIMIFQPLEGSPAAEAGVHANDRIIRIDGVATK
                     GMSLAEAAQLIKGPRGTVVKLTLLRPAAADNGENEILEVDIKRGTIPLESIVEARVLE
                     GGIGYLRLSDFKRTTDDELRKTLKDLDSQGMKSLVLDLRWNPGGLLDSSVDVCSLFLE
                     ANTLVTYTKERDLDTGELREDMMLYTKGGPAEPISFPLVILVNQFTASSSEIVTGALQ
                     FHKRAIIVGQRTFGKGSVQTIIPLRRPAGSALRLTTALYYTPAEVTIHKQGILPDVEL
                     EMPEAHQRQLLDQMRQSFIGDPGKRRNGQNHGTITGDAATESSVEDTQLQRAVDILRE
                     DPVFENLINKYHRDTKETQVAAAEEKMRATPPSSSPAGLSSGDASQAGELESPKEQE"
     gene            1186..>1671
                     /gene="rph"
                     /locus_tag="PKW60_14965"
     CDS             1186..>1671
                     /gene="rph"
                     /locus_tag="PKW60_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPK00786.1"
                     /translation="MRPDNRPNDALRPVLFERQFTRYAEGAVLVSYGNTKVLCTASVE
                     DRVPAFLRDSGHGWITAEYAMLPRSTHTRTSRESFNAGRAKEISRLIGRSLRNVVDMS
                     FLGERQITLDCDVIQADGGTRTAAITGAYVALHDAIRHLFDTGGIAKMPLLGQCAAVS
                     VG"
BASE COUNT          356 a          514 c          512 g          289 t
ORIGIN      
        1 tatcggcgtg acgattcagc tcgacgagaa cggcaacatc atgatctttc agccgctgga
       61 aggttccccg gccgccgagg ccggagtcca tgccaacgac cggatcatcc gcatcgacgg
      121 ggtggcgacg aagggcatga gcctggccga agcagcgcag ctgatcaaag gcccccgcgg
      181 caccgtcgtt aaattgacgc tgctccgccc cgctgccgcc gacaacggtg aaaatgaaat
      241 ccttgaagtg gacatcaagc ggggcacgat cccgctggag agcattgtcg aagcccgcgt
      301 actggagggc ggaatcggtt atttgcggct gagcgacttc aaacgcacca cggacgacga
      361 actgcgtaag accctcaaag acctggacag ccaggggatg aaatcgctgg ttctggacct
      421 gcgctggaat cccggcggtc ttctcgattc gtccgtagac gtgtgcagcc tctttcttga
      481 agccaatacc ctggtgacgt acacaaagga acgtgacctc gatacgggcg aactgagaga
      541 agacatgatg ctctatacca agggcgggcc cgcggagccc atttcatttc cactggtgat
      601 ccttgtgaat cagttcaccg ccagctcgtc ggaaatcgtc accggagcgc tccagttcca
      661 taaacgggcg atcatcgtcg ggcaacggac ctttggcaag gggagcgttc agaccattat
      721 cccgttgcgg cgcccggccg gcagcgcgct ccggctcacg accgccctgt attacacgcc
      781 cgcggaagtc accattcaca aacagggcat ccttccggac gtcgagctcg aaatgccgga
      841 ggctcaccag cggcagttgc tcgaccagat gcggcaatcc ttcattggtg acccgggcaa
      901 gcggcgcaac ggccagaatc atggcaccat taccggcgac gccgctacgg aaagcagtgt
      961 cgaagacacg cagctccagc gcgcggtcga tattctgcgc gaggacccgg tcttcgaaaa
     1021 tctcatcaac aaataccatc gcgacaccaa agaaacccag gtggcggcgg cggaagagaa
     1081 gatgcgggcc acgccgccat cttcgagtcc ggccgggctt tcttctggag acgcgtcgca
     1141 ggccggtgag ctcgagagtc cgaaggagca ggaatagagt tgtccatgcg ccctgataac
     1201 cggccgaatg acgcgctgcg cccggtgttg ttcgagcgtc agtttacgcg ttacgccgaa
     1261 ggcgccgtgc tggtgtcgta cgggaacacg aaagtcctct gtaccgcaag cgtggaagac
     1321 cgggtgcccg cgtttttacg cgattccggg catggctgga tcaccgcgga atacgccatg
     1381 ctgccccggt caacccatac gcgaacgtcc cgggagtctt tcaacgccgg gcgcgccaaa
     1441 gagatcagcc gcctgatcgg caggtcgctg cgcaacgtgg tcgatatgag ctttctcgga
     1501 gaacggcaga tcaccctgga ctgcgacgtg attcaggcgg atggcggcac ccgcaccgcg
     1561 gcgattaccg gcgcgtatgt tgcgctgcac gatgcaatcc gccacctgtt tgatacgggc
     1621 gggatcgcca aaatgcccct cctcggccaa tgcgcggccg tgagcgtggg c
//
LOCUS       DAOPBQ010000635         1663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_58141_length_1663_cov_4.734453, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000635 DAOPBQ010000000
VERSION     DAOPBQ010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58141_length_1663_cov_4.734453"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKW60_14970"
     CDS             complement(<1..299)
                     /locus_tag="PKW60_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HPK00787.1"
                     /translation="MKIFAGTASSGLSERICAHLGMACGNAAVTHFEDGEIRVQFQEN
                     IRGKDVFLVNSTSPPVNDRLIELLILIDAARRASAARITAVLPYFGYARQDRKDRP"
     gene            complement(438..512)
                     /locus_tag="PKW60_14975"
     tRNA            complement(438..512)
                     /locus_tag="PKW60_14975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Gln,seq:ttg)
     gene            complement(904..>1663)
                     /locus_tag="PKW60_14980"
     CDS             complement(904..>1663)
                     /locus_tag="PKW60_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00788.1"
                     /translation="CRWLFGAAAEMADFLDEQDDAARWRTVLAEFPELYLSENGALLL
                     AKNHPLPFSHRHHSHLMAMYPLGLVDAANGEGDVRTMRASLEELDQFGPGAWVGFSYT
                     WLASLAARARDGERAARALDIFAEAFVLRNSFNANGDQTGKGYSDFTYRPFTLETNCS
                     NASALQEMLLQSHTGVIDVFPAIPADWNDVAFHQLRAQGGFLVSAEKRAGKLVSLTIQ
                     AERGGTARVRCPGLDTLQILELAPGAVHDLAGAG"
BASE COUNT          327 a          533 c          492 g          311 t
ORIGIN      
        1 ggccggtctt tacggtcctg ccgcgcatac ccgaaatacg gcaacacggc tgtgattcgc
       61 gccgcggacg cccgtcgtgc cgcatcaatc agaatgagca gctcgatgag gcgatcgttc
      121 acgggcggtg acgtgctgtt gacaaggaat acgtccttcc cgcggatgtt ttcctgaaac
      181 tggacgcgga tttctccgtc ttcgaaatgg gtaaccgcgg cattaccgca ggccatcccc
      241 aggtgggcgc aaatgcgctc gctcaggccg ctgctggctg tgccggcaaa gatcttcatt
      301 tcatcgctgt acgccaaaat acaccaccct tgcgttcgcc cgtctctgcc cggttttccg
      361 gcatgacacc accagaacca cgaacgggca gccgcctcgg cgccagtatc cgcgcctcac
      421 agccgggcac aacaaattgg ctggggcggt aggattcgaa cctacgaatg tcggctccaa
      481 aggccgatgc cgtacctctt ggctacgccc cagcagataa agcccctgaa ccccggctcc
      541 acgcatgacg ggccgctccc gggttcattg gtgtaagtgg gctgtctgaa gcgcgttggc
      601 ttgccaaagc gaaagcaaag tataccaacg ggttcccggc caagtcaatt tgccgaatcg
      661 cgcccatttg tgatcgcccg gggagaatac cgtgctgcca gttgctatct tgccggagca
      721 aggggtaccc ggaaagcgga tttcggccga atcgcctgtc gggcgtgtta tgcacaggcg
      781 aaacgcctgc ttcgcgggcc cggcccgtgg ctcactgcgg ccatcggcat tatgtgtgaa
      841 tttgtcgcga tttactcggg aaacccgaaa aacaggcact gcagcccttt tgaatcctcc
      901 tgtctagccg gcacccgcca aatcgtgcac tgcgccgggc gccagctcca gaatctgcag
      961 ggtatccaaa cccgggcagc gcacccgcgc tgtgccgccg cgctccgcct gtatggtgag
     1021 cgagaccagt tttcccgccc gtttctcggc cgaaacgagg aaaccgcctt gcgcccggag
     1081 ctggtgaaag gcaacgtcgt tccaatccgc gggaatggcg ggaaacacgt cgatcacgcc
     1141 cgtatggctc tggagcagca tttcctgcag ggcggatgca ttcgaacaat tggtttcgag
     1201 cgtgaacgga cggtaggtga aatcgctgta tcccttgccg gtctgatcgc cgttggcgtt
     1261 gaagctgttg cgcaacacaa aggcctccgc aaagatgtcg agcgcccggg cggcgcgttc
     1321 gccatcgcgg gcgcgcgcgg cgagactggc cagccacgta tagctgaatc ccacccatgc
     1381 tccaggccca aattggtcga gttcctccag cgaggcgcgc atggtacgca catcaccttc
     1441 gccattcgcc gcatccacga gtcccagagg atacatggcc atgagatgcg agtgatgccg
     1501 gtgcgagaac ggcagcggat gattcttggc caggagcagc gcgccgtttt cgctcaggta
     1561 cagttcagga aattccgcga gaaccgtgcg ccagcgcgcc gcgtcgtcct gctcatccag
     1621 aaaatccgcc atctccgcgg ccgcgccgaa cagccagcgg cac
//
LOCUS       DAOPBQ010000636         1658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_58403_length_1658_cov_4.239551, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000636 DAOPBQ010000000
VERSION     DAOPBQ010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58403_length_1658_cov_4.239551"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PKW60_14985"
     CDS             <1..866
                     /locus_tag="PKW60_14985"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPK00789.1"
                     /translation="HGATGKGNDQVRFELTYLALEPSVAIIAPWRDPRWDLTSRTKML
                     EYAEKHGIEVPVTVKKPYSNDRNLLHLSFEGGILEDPWAEPPADMFQLTVDPAEAPDA
                     PTYLEIEFEEGTPVAVDGERLSPAELLARLNTVAGANGVGRVDMVENRFVGMKSRGVY
                     ETPGGTVLYAAHRAVESITMDREVMLQRDQLSPKIAQLIYNGFWYSPEMQALMAFVDA
                     SQQNVTGTARIKLYKGNCTVVGRKAPKSLYDEKISTFEEDEGAFQQADAGAFIRLNAL
                     RLRVRNRAGLL"
     gene            890..>1658
                     /gene="argH"
                     /locus_tag="PKW60_14990"
     CDS             890..>1658
                     /gene="argH"
                     /locus_tag="PKW60_14990"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPK00790.1"
                     /translation="MAKLWGGRFQGQADALVEALGQSVSFDARLAPWDIRGSIAHARM
                     LGKCGIIPQRDARKIIDGLSEIAKDIESGVFVFDPALEDVHTNIESALVKRIGDAGKR
                     LHTGRSRNDQIALDVRLWMRDQIDVIRARLRALQRAFLDLAEAHAGVILPGCTHLQHA
                     QPVLLAHHLLAYVEMLQRDHERFSQMRARVNVMPLGSAALAGTPYPIDRKMVADELGF
                     PRVSQNSMDAVADRDHLIEFCAGAALCMMHLSRWCEEL"
BASE COUNT          326 a          533 c          505 g          294 t
ORIGIN      
        1 cccacggcgc cacgggcaag ggcaacgatc aggtccgctt cgaactcacc tatctggcgt
       61 tggaaccttc ggttgccatc atcgcgccat ggcgcgatcc ccggtgggac ctgaccagcc
      121 gcaccaagat gctcgagtac gcggaaaaac acgggatcga agtgcccgtg accgtcaaga
      181 aaccctattc caacgaccgc aacctgctcc atctctcgtt tgaaggcggc atcctcgaag
      241 atccctgggc cgaaccgccc gcggatatgt tccagctgac cgtcgacccc gctgaggcgc
      301 ccgatgcgcc cacctacctc gaaatcgagt tcgaggaagg cacgcccgtc gcggtagatg
      361 gcgagcggct ctccccggcg gaattgctgg cccggctgaa cacggtcgcg ggcgccaatg
      421 gcgtcggccg cgtcgatatg gtggaaaacc gcttcgtggg catgaagtcg cgtggtgtat
      481 acgagacgcc cggcggcacg gtcctctacg cggcgcatcg cgcggtcgaa tccattacaa
      541 tggatcgcga agtcatgctc cagcgcgatc agctgtcgcc gaagattgcc cagctgatct
      601 acaatggatt ctggtattcg ccggagatgc aggccctcat ggccttcgtc gacgcatcgc
      661 aacagaatgt cacgggtacg gctcggatca aactctacaa gggcaactgc accgtggtcg
      721 gacggaaagc gccgaaatcc ctgtacgacg agaagatatc tacgtttgaa gaggatgagg
      781 gagcgtttca gcaggccgat gccggcgcgt tcattcgttt gaatgctctc cggctgcgcg
      841 tgcgcaaccg ggccgggctc ctgtagcgaa aacgctcgag ggttgtcgca tggcaaaact
      901 ctggggtggc cgttttcaag gtcaggcgga tgcgctggtg gaagcgctag gccaatccgt
      961 gtcatttgac gcccggctgg cgccatggga catccgcggc agcatcgccc acgcgcgcat
     1021 gctgggcaag tgcggcatca tcccacagcg cgatgcccgg aaaatcatcg acggcctcag
     1081 cgagattgcc aaagacatcg aaagcggcgt gtttgtcttc gaccctgcgc tggaagacgt
     1141 ccacaccaat atcgaatccg ccctggtcaa acgcattggc gatgcgggca aacgcctcca
     1201 caccggccgc agccgcaatg atcagatcgc gctggacgtc cggctctgga tgcgcgacca
     1261 gatcgacgtg atccgggcgc gtctgcgcgc actgcaacgg gcctttcttg acttggccga
     1321 agcccacgcc ggggtgatcc tgccgggatg cacgcacctc cagcacgccc agcccgtcct
     1381 gctggcccat cacctcctgg cctatgtgga aatgctccag cgcgatcatg agcgtttcag
     1441 ccagatgagg gcgcgggtca atgtgatgcc gctcggctcc gccgcacttg ccggcacgcc
     1501 ctatcccatc gaccgaaaga tggtggccga tgaactggga ttcccgcgcg tgtctcagaa
     1561 cagtatggat gccgtggccg accgcgacca cctcatcgaa ttttgcgccg gcgccgcgct
     1621 gtgcatgatg cacctctcgc gctggtgcga agaactca
//
LOCUS       DAOPBQ010000637         1635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_59668_length_1635_cov_2.535443, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000637 DAOPBQ010000000
VERSION     DAOPBQ010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59668_length_1635_cov_2.535443"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PKW60_14995"
     CDS             complement(<1..321)
                     /locus_tag="PKW60_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00791.1"
                     /translation="MTCSAWTLAAALLIIPLAGQESQGAASPTVSVQLIAVRATSQGH
                     EKAQFDSNIAFIRETVEDLKQFDDFRKLKSVNTTAPYGKETKVLLDDRYTLLIHPIER
                     DSLDR"
     gene            complement(318..761)
                     /locus_tag="PKW60_15000"
     CDS             complement(318..761)
                     /locus_tag="PKW60_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00792.1"
                     /translation="MNRKLRTIERWFDGATADEAEAQTLVAGKPEYAAHLEALKLLRT
                     GARSLAQQEVIEPAQFPAFMAGIRERLEPAPRHHRGVWALASAVAASLIAAIAIYMMA
                     APPPENISAEVEQVGTTIEGAEVGYYNTDNGTMTVWVDIPEGDLP"
     gene            complement(758..1402)
                     /locus_tag="PKW60_15005"
     CDS             complement(758..1402)
                     /locus_tag="PKW60_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK00793.1"
                     /translation="MSIALDTTVLCDISQDEEQEPRVMVRAESDDADLVARAKTGDVD
                     AFELLVKRYRNEVFALAYHFVRSREQAWDVSQEVFIKAHNALARFRGDASFKTWLMRI
                     TANQCKDYLKKRRLETVSFDDSRLAEDAPSHVLPPDRSLEAEEIGAAVQKALGKLSHK
                     HRTAFILREFEGLSYEEMAGVMNCSIGTVMSRLHHARKKLQKSLTQMGFMGADA"
BASE COUNT          314 a          481 c          489 g          351 t
ORIGIN      
        1 acggtcgagg gaatcccgtt cgatgggatg gatgagcagt gtgtagcgat catccagaag
       61 aaccttggtt tctttgccat agggggccgt ggtattcacg cttttcagct tgcggaaatc
      121 gtcaaactgt ttgaggtctt ccacggtctc ccggatgaat gcgatgttcg agtcaaactg
      181 ggctttttca tggccctggc tcgtggcacg cacggcaatc aactgcacag acacggttgg
      241 cgaagccgcc ccctgggatt cctgcccggc caggggaatg atcaacaatg cagcggcaag
      301 cgtccaggct gagcaagtca agggagatct ccttccggga tatccaccca aaccgtcatc
      361 gtgccgttat cggtattgta gtaaccgact tccgcgcctt cgatggttgt gccgacctgt
      421 tccacttcgg cggatatgtt ttcaggaggg ggcgcagcca tcatatagat cgcaattgcc
      481 gcgataagcg aagcagcgac tgcggacgcc agggcccaga cgccccggtg gtgccgcgga
      541 gcgggctcga gccgttcccg tatgcctgcc atgaaggccg ggaactgtgc cggttcgatg
      601 acctcctgct gtgccaggct gcgcgcaccc gtgcgcaaca gcttcaaggc ttccagatgg
      661 gccgcgtatt cgggcttgcc ggcgacgagg gtctgagcct cggcttcgtc tgccgtggca
      721 ccgtcaaacc agcgttcgat agtcctgagt ttccggttca tgcgtccgct cccataaagc
      781 ccatttgagt caggctcttt tgcagcttct tgcgcgcgtg gtgcagccgg ctcatcaccg
      841 tgccgatgct gcaattcatc actccggcca tctcctcata cgagaggcct tcaaattcac
      901 gtaaaataaa cgctgtccga tgcttatgcg acagcttgcc gagtgccttc tgcacggcag
      961 cgccgatttc ctcggcttcg aggctgcggt caggcggaag cacgtgactg ggggcgtctt
     1021 ccgcgagccg actgtcatca aacgagacgg tttccagccg ccgcttcttc aaataatctt
     1081 tgcactggtt cgcggtgatg cgcatcagcc acgttttgaa actggcgtcg ccgcggaaac
     1141 gtgcgagggc gttatgggcc ttgatgaaca cctcctggga gacatcccac gcctgttcgc
     1201 ggcttctcac gaagtggtag gcgagtgcga aaacctcgtt acggtaacgc tttacgagca
     1261 attcgaaggc gtcgacgtcg ccggtttttg cacgggcgac caggtccgcg tcgtcagact
     1321 cggcccgtac catcacccgc ggctcctgct cctcgtcctg cgagatgtcg caaagaaccg
     1381 tcgtatccag cgcgatactc aattgtccac tctcctcacc cgaacgatac ggacgattat
     1441 cgggcagaaa tatttaatag atttcgcgtt atgctttccg cgagaggttc cacggcgtgc
     1501 atgatacccc cgcaggaggg acggcgtaaa gtggccccag aggccgctac caggttcggg
     1561 cgattacgaa atcgacaatg cccgcaaggg aagtgcggta cgggcccggg ggaagctggg
     1621 cggcagcggc gtggg
//
LOCUS       DAOPBQ010000638         1632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_59789_length_1632_cov_4.245403, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000638 DAOPBQ010000000
VERSION     DAOPBQ010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59789_length_1632_cov_4.245403"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKW60_15010"
     CDS             <1..443
                     /locus_tag="PKW60_15010"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPK00794.1"
                     /translation="TRIVRIFNTYGPRMRPDDGRVVTNFACQALQGHPLTLYGDGNQT
                     RSFCYVSDLVEGFYRLLISDETGPVNLGTPDEYTVREVAEMIIELTGSPSEIICVPQP
                     FPDDPRRRKPDITRARQVLGWEPEVELRDGLRRTIESLQRELQP"
     gene            complement(470..1156)
                     /locus_tag="PKW60_15015"
     CDS             complement(470..1156)
                     /locus_tag="PKW60_15015"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPK00795.1"
                     /translation="MRKAVAVAMLAVLTALWVEALYAGAKKPLPRPTGAVTPVPSGEG
                     WVDLFDAENARLWEVNAKDLFEFQDGVMHIFGKKPTHYIQYTGDTYGDFQAHLEFNLT
                     AKANSGLMFRAAPEDPVYRGMEIQIIDSYGEDPSYFSCGALYDVASPMFNVVNKPGEW
                     NSLDLTVQARHVVAVVNGWKVLDVDLSLMTMPIGKFPTPLDELPTTGSLFLQDHGNEV
                     WFRNMRVKRL"
BASE COUNT          313 a          533 c          460 g          326 t
ORIGIN      
        1 atacccgcat cgtgcgcatt ttcaacacct acggtccccg catgcggccc gacgacgggc
       61 gcgtcgtcac gaatttcgcc tgccaggcgc ttcagggcca tccgctgacc ctgtacggag
      121 acggaaacca gacccgaagc ttttgttacg tctccgacct ggtcgaggga ttttaccgcc
      181 ttttgatctc cgacgagacc ggccccgtga atctcggcac tcccgacgag tacaccgtgc
      241 gggaggtggc ggagatgatc atcgagctga cgggaagccc aagcgagatc atctgtgtcc
      301 cccagccgtt tcccgacgat ccccgccggc gcaagcccga catcacccgg gcgcgtcagg
      361 tgctgggctg ggagccggaa gtggaactcc gcgacggcct tcgccgcacc attgaatcgc
      421 tccagcgcga gctccagccc taggcgcgcc gcggcccgcg ggtactgagt tacagtctct
      481 tcacacgcat atttctgaac caaacctcgt tgccgtggtc ctgaagaaac aggctgccgg
      541 tcgtgggaag ttcgtccaat ggggtcggaa acttgccgat cggcatggtc atcagcgaca
      601 aatccacatc cagcaccttc cagccattca ctacggccac gacgtgcctg gcctgcacgg
      661 tcaaatcgag cgagttccat tcaccgggct tgttaacgac gttgaacatc ggcgaggcca
      721 cgtcgtacag cgctccgcaa ctgaaataac tcgggtcttc tccgtaggaa tcaatgatct
      781 gaatttccat ccccctatag accggatcct cgggggccgc gcggaacatg agcccgctgt
      841 tggcctttgc cgtcagatta aactccagat gcgcctgaaa atccccgtag gtgtccccgg
      901 tgtattggat gtaatgggtg ggtttcttcc cgaagatgtg catcacaccg tcctggaact
      961 caaacaggtc cttcgcattg acctcccaca gccgggcgtt ttccgcatcg aacaggtcca
     1021 cccagccttc ccccgaggga accggcgtga ccgcgcccgt tggacgcggc agcggctttt
     1081 tggcccccgc gtataaggct tcaacccaca gtgccgtcag caccgccagc atggcaaccg
     1141 ctaccgcctt tcgcatggtt tccgtccttt cccctccgta tgaggatctg cacaaaagag
     1201 cataacgcag cgacggccga aaacgcacgc cgctgtatcc ttccggcaga cgcgaaaacc
     1261 ctcgccgtgg aacctctgcg cgctgccgct gtagaacgct tcggcggcgg actgcggcgc
     1321 cacctccagc aaataatttg tgtatctgtg acgcgccggc ttgtcgtata cttcgcgcac
     1381 gctggactgc cttggggata ccgcgtccct gctccagggc ggccacggca aaaatgtgta
     1441 tcatggctgc agcttcaagg cactccaggg catcgaaagg ccgggtagat acgtgaacgt
     1501 gaagtatgcg cagttgctgt ttctgctgtt tttcggattg gccgtgttcg tgcctgccat
     1561 cggcacccac gatctgtggc cggccgacga gccgcgttat gcccaggtcg cccgtgagat
     1621 gctggagacc gg
//
LOCUS       DAOPBQ010000639         1626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_60110_length_1626_cov_3.216423, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000639 DAOPBQ010000000
VERSION     DAOPBQ010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60110_length_1626_cov_3.216423"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKW60_15020"
     CDS             complement(<1..702)
                     /locus_tag="PKW60_15020"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK00796.1"
                     /translation="MTEDAFATLARYYDRIMAHVDYDRWYLASLALGEMLPAPFRHAD
                     LACGTGTLVKRLHRAQWDTVGLDLSPAMLRMGRKSGHPWRSIVGDLRALPFRETFGLI
                     TCLFDSINFLLSESDMAAAIQQCGNALRPGGLFYFDYVTEEMVLRHFADQNWTENNGA
                     FSTRWENMYDRSSRITETTIRINGGVANIILERIHSQAAITSALAKAGLHPLAFLDAE
                     TWKPPGQKTIRVDVVA"
     gene            complement(699..1343)
                     /gene="yqeK"
                     /locus_tag="PKW60_15025"
     CDS             complement(699..1343)
                     /gene="yqeK"
                     /locus_tag="PKW60_15025"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HPK00797.1"
                     /translation="MEPTNHSIAIPRAAEYIALLEQRLSPRTMMHTLSVAACMASLAS
                     LAGVPGEAAITAGLLHDLKKDTGKETLLREALAYGLDVSPIQRENPKLLHGPVAAETC
                     RRELGVTDDAVYEAIYWHTTGRPGLGRTGLLLYFADYAEPLRDRPDAARARERCQTEG
                     FFRALYFVACQKVQYIRTRGSVDPISEDFFAWLGQELLRQGHLSEHECAVGLLQ"
     gene            complement(1324..>1626)
                     /locus_tag="PKW60_15030"
     CDS             complement(1324..>1626)
                     /locus_tag="PKW60_15030"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPK00798.1"
                     /translation="QRQYPAAQLFLILGLDSLAELPKWRHPERILAVAHVLAAPRPGA
                     YQIPEILNGRYTLLELEPVEVSSTEVRNRAANREPYEELLPPPVRRYVKKHGTYES"
BASE COUNT          307 a          507 c          510 g          302 t
ORIGIN      
        1 tgcgaccaca tccacccgta tggtcttctg gccgggaggt ttccaggtct ccgcatccag
       61 aaacgcaaga gggtgcaacc ccgctttcgc cagggccgac gtgatggctg cctgcgagtg
      121 tatccgctcg agaatgatgt ttgccactcc gccattgatt cggatggtgg tctcggtaat
      181 ccgcgaggac cggtcataca tattttccca tcgcgtactg aaggccccgt tgttttccgt
      241 ccagttctga tccgcaaaat ggcgcaaaac catctcctcg gtcacataat caaaatagaa
      301 cagaccgccg ggccgcaggg cgttgccgca ctgctggatg gccgcggcca tgtcggactc
      361 gctgagcagg aagttgatgc tgtcaaaaag gcaggtgatc aacccgaatg tctcgcgaaa
      421 cggcagcgcc cgcaggtcgc cgacaatact gcgccaggga tgcccgctct ttcggcccat
      481 gcggagcatg gcaggggaga ggtcaagccc gacggtgtcc cattgggccc ggtgaagccg
      541 cttcactaag gtgcctgtac cacaggccag atcggcatga cgaaacggcg caggcagcat
      601 ctctcccagc gcgagggacg ccagatacca ccggtcatag tcgacatggg ccatgatgcg
      661 gtcgtagtac cgcgccagcg tcgcgaacgc atcttccgtc attgcagcag ccccacggcg
      721 cattcatgct ccgagaggtg gccctggcgc agcagctcct ggccgagcca cgcaaagaaa
      781 tcttccgaaa tggggtctac cgagcctcgg gtccggatgt actgcacttt ctggcaggcc
      841 acgaaataga gcgcccggaa aaacccttcc gtctggcagc gctcgcgggc acgagcggca
      901 tccggccggt cgcggagcgg ctcggcgtag tccgcgaagt acagcaacag gcccgtgcgc
      961 cccagtcccg gccgcccggt cgtgtgccag tagatggcct cgtacaccgc gtcgtcggtc
     1021 acgcccaatt cgcgacggca cgtctcggct gcgacggggc cgtgcaataa tttcgggttt
     1081 tcgcgctgaa tgggggagac atccaatcca tacgccagag cttcgcgaag cagggtctct
     1141 ttgccggtgt cctttttgag atcgtggagc aaccccgctg tgatcgcagc ttcgccggga
     1201 acgccggcca ggctcgccag cgacgccatg caggcggcca cggacagtgt atgcatcatc
     1261 gttcgcgggg acagccgctg ttcgaggagg gcgatatact ccgcggcccg tgggatggca
     1321 atactatgat tcgtaggttc catgcttttt gacataccgg cgcaccggcg gtggcagcaa
     1381 ttcttcgtac ggctcccggt ttgccgcgcg attgcgtact tccgttgagg agacttcgac
     1441 cggttcgagc tccagcaagg tatagcggcc gttcaatatc tcggggatct gataggcacc
     1501 cggcctgggg gcggccagca cgtgggccac cgccaggatg cgctcggggt ggcgccattt
     1561 cggcagctct gccagcgaat ccaagccgag gatcaggaac agctgcgcgg ccgggtactg
     1621 ccgttg
//
LOCUS       DAOPBQ010000640         1602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_61406_length_1602_cov_3.526180, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000640 DAOPBQ010000000
VERSION     DAOPBQ010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61406_length_1602_cov_3.526180"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKW60_15035"
     CDS             <1..348
                     /locus_tag="PKW60_15035"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPK00799.1"
                     /translation="LQQALEMVPEHPGLFRAATCLDAGAAPGAKLSCGCFTGAGIVIG
                     YICGGENFSDRSLAHKLIQQVARKFQKTYGSVLCEDARKGGKCHVVVGRAVQWTAEVL
                     LAQFCGYTSPSQG"
     gene            complement(370..1170)
                     /locus_tag="PKW60_15040"
     CDS             complement(370..1170)
                     /locus_tag="PKW60_15040"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HPK00800.1"
                     /translation="MKTNHHTHTFRCKHATGNVDDYCRAALEAGLTVLGFSDHAALPD
                     NRWLSVRMEYDEREAYCADVEQARHAFPGLSILNAMECEYTPAYRAYFEDELLGRLEL
                     DYLVGAAHFIAHNGSWLPVYGGITDAAALRTYASVYCTLIEWGLFAFIAHPDLFGNSY
                     LAWDRHAEACSREILQTAEAHRVPLEINGYGFRKPWLETPEGCRPMYPWGRFWELAAE
                     YDIEVVINSDAHRPEDVAANLDDAAAIAERFGLRVADLSCLESERHGR"
     gene            complement(1220..>1602)
                     /locus_tag="PKW60_15045"
     CDS             complement(1220..>1602)
                     /locus_tag="PKW60_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HPK00801.1"
                     /translation="PRSQNLNTENGVLIEDAKIAQTVREAILSSTQPGRSWVIAKRRF
                     PLSDVNYLIEGLSGLSPVDLWPLRNTTSYELRPGMEPVDPGDEDFYARYDDVGSFPGA
                     EGVSTKQIVTRIYKMLGSLAVPIL"
BASE COUNT          305 a          476 c          512 g          309 t
ORIGIN      
        1 ctgcagcagg cgctcgagat ggtgccggag catcccggcc tgttccgcgc agcgacctgc
       61 ctcgatgccg gcgccgcgcc gggcgcgaag ctgagctgcg gctgtttcac cggcgcgggc
      121 atcgtgatcg gctatatctg cggcggagag aatttcagcg acaggagcct cgcgcacaag
      181 ctgattcagc aggtggcccg aaagtttcag aaaacctacg gcagcgtcct gtgcgaggat
      241 gcccgaaaag gcggcaagtg ccacgtggtc gtgggacgcg ctgtccagtg gaccgccgag
      301 gtactgttgg cgcagttctg cgggtacacg tctccgtctc agggatgagg agagccgggc
      361 ggcctggcgt caccgcccat gccgctcaga ttcgaggcag gacagatcgg ctacccgcaa
      421 tccaaatcgt tccgcgattg cggcggcgtc gtccaggttt gccgcgacgt cttcgggccg
      481 gtgggcatcg gaattgatca caacctcaat gtcgtattct gcagccagtt cccagaagcg
      541 gccccaggga tacatcggcc ggcagccttc gggcgtctcg agccagggtt tgcggaaccc
      601 atagccgttg atctccagcg gcacgcgatg agcttccgcc gtctggagga tctcgcgcga
      661 acaggcctcc gcgtgccgat cccaggccag gtagctgttc ccgaacagat cgggatgcgc
      721 gataaacgca aataagcccc attcaatgag cgtgcagtac acggacgcat aggttcgcaa
      781 tgccgcggcg tctgttattc ccccgtagac gggaagccag ctcccgttgt gtgcaatgaa
      841 gtgcgccgcg ccgaccaggt agtcaagttc gagccggccg aggagttcgt cctcgaaata
      901 cgcccggtac gcgggcgtat actcgcattc catggcgttc aggatgctga ggccggggaa
      961 ggcgtggcgg gcctgctcga cgtcagcgca atacgcttcc cgctcatcgt attccatccg
     1021 gaccgacagc cagcggttgt caggcagcgc ggcgtgatcg gaaaatccaa gtacggtcag
     1081 gccggcctcg agggccgcgc ggcaatagtc gtcgacgttg cccgtagcat gtttgcaacg
     1141 gaatgtatgg gtatgatgat tggtcttcat ggattccttt cgagggaaga atcgggggtt
     1201 ttgcatgcat gcctgcccat tataggatag gcacggccag agaacccagc attttgtaga
     1261 tccgcgtcac gatctgtttg gttgagacgc cctcagcgcc ggggaaactt cccacgtcgt
     1321 cgtaacgcgc ataaaaatct tcatccccgg ggtcaacagg ctccatgccg ggccgcaact
     1381 cataactggt ggtgttgcgc aggggccaga gatctaccgg cgacaaccct gacaatcctt
     1441 cgatcaggta gttgacatcg gacaacggaa accgccgctt ggcaatcacc cagctccggc
     1501 cgggctgggt gctgctgagg atggcctcgc gcacggtctg ggcgatcttc gcatcttcga
     1561 tcagcacgcc gttctcggtg ttcaagtttt gtgagcgggg at
//
LOCUS       DAOPBQ010000641         1595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_61826_length_1595_cov_2.974026, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000641 DAOPBQ010000000
VERSION     DAOPBQ010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61826_length_1595_cov_2.974026"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKW60_15050"
     CDS             <1..283
                     /locus_tag="PKW60_15050"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPK00802.1"
                     /translation="HRVIFGCADGDIHGLERESGTPVWTVETGGMATSPVIARDKVVV
                     GSRGTLLLLRLDGGEKLWSHEVSDEITAPAVYDGLVIVGCDDGTVAAFR"
     gene            308..1003
                     /locus_tag="PKW60_15055"
     CDS             308..1003
                     /locus_tag="PKW60_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK00803.1"
                     /translation="MNESAVLLEMRDVTKSYPASPAPLDVLKGVSLHLKQGESVAVVG
                     PSGCGKSTLLNLIGTLDTPSSGSIYFEGRDLGGLNSQELARFRNTSVGFVFQSHHLLP
                     QCTALDNVLVPALVNDGAQVRKARALDLLERVGLLERAAHRPGQLSGGERQRVAVVRA
                     LINQPRLLLADEPTGALNEEAAAGLVALLLELSREEGMTLVVVTHAKQVAAQMDRVLE
                     LHDGLLKPEEPAA"
     gene            1000..>1595
                     /locus_tag="PKW60_15060"
     CDS             1000..>1595
                     /locus_tag="PKW60_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00804.1"
                     /translation="MSMLRLVWNGLRFHWRMHAGTAGGIALAAAILTGALLVGDSADY
                     SLRTYALARLGSIHYAIESRSGFFSEAYVTGLREQMPARAVPVLTLPGMALAGDGSDT
                     RQINQANVIGISPEFEELEPNAAVSPGEHEAILNERLAEALGVQTGDHVALRILKPGL
                     LPRDAPLSSRAGDSTVRALCTISAVLPDTGLGRFSLSPA"
BASE COUNT          264 a          482 c          554 g          295 t
ORIGIN      
        1 gcaccgggtc atctttggct gcgccgatgg cgatatccat gggctcgaac gcgaatccgg
       61 cacgccggtg tggacggtgg agacgggtgg catggcgacc tctcccgtta tcgccagaga
      121 caaggtggtc gtgggttcga gaggcacgct gcttctgctc cggcttgacg gcggggagaa
      181 gctgtggtcc catgaagtca gtgacgagat caccgcgccg gcggtgtatg acggcctggt
      241 gattgtgggt tgcgacgacg gaaccgtggc cgcgttccgg tagttggcgg ccgggaggcg
      301 tctggagatg aatgagtcgg cggtcctgct tgaaatgcgc gacgtgacga agtcgtatcc
      361 cgcgtcgccc gctcctttgg atgtcttgaa aggcgtcagc ctgcatttga agcaggggga
      421 gtccgtcgcc gtggttgggc cgtcgggttg cggcaaaagt acgctgctga acctgatcgg
      481 cacgctggac acgcccagtt ccggctcgat ttacttcgaa ggccgcgatt tgggcggctt
      541 aaactcacag gaactggcac ggttccgaaa cacctccgtg ggattcgtat ttcagtccca
      601 ccatctcctg ccccaatgca cggcgctgga caacgtgctg gtgcctgcgc tcgtgaatga
      661 cggcgcgcag gtcaggaagg cgcgcgcgct ggacctgctc gaacgcgtcg gactcctgga
      721 gcgcgcggcg catcgccccg ggcaactttc cggcggagag cgccagcgcg tcgctgtggt
      781 gcgggcgctg atcaaccagc cgcgactgct gctggccgac gagccgacgg gcgcgctcaa
      841 cgaggaggct gcggcggggc tcgttgcgct cctgctcgaa ctcagccggg aagagggcat
      901 gacgctggtg gtcgtcacgc atgcgaagca ggttgcagcc caaatggacc gggttctcga
      961 attgcacgac gggctgctca aaccagagga accggcggca tgagcatgtt gaggctggtg
     1021 tggaacggcc tccggttcca ctggcggatg cacgcgggca ccgccggggg tatcgcgctg
     1081 gcggccgcaa tccttaccgg cgcgcttctg gtgggcgaca gcgcggatta ctcgctgcgc
     1141 acctatgcgt tggcgcgcct gggctcgatc cactatgcca tcgagtcgcg cagcggcttc
     1201 ttctcggagg catatgtcac gggtctgcgc gaacagatgc ctgcccgcgc tgttcccgta
     1261 ctgaccctgc ccggcatggc cctggcggga gatgggagcg atacacggca gatcaaccag
     1321 gccaatgtga tcggtatttc acccgaattc gaggaactgg agccaaatgc cgctgtatcg
     1381 ccgggcgaac acgaggctat cctgaacgag cgcctcgcgg aggccctggg cgttcaaaca
     1441 ggggaccatg ttgcgttgcg catcctcaag ccggggcttc ttccacgaga tgcgccgctt
     1501 tcttcacgcg ccggagattc cactgtgcgc gcgctgtgca cgattagcgc ggtactgccc
     1561 gacaccgggc tgggccgctt cagcctcagt ccggc
//
LOCUS       DAOPBQ010000642         1579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_62733_length_1579_cov_3.888451, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000642 DAOPBQ010000000
VERSION     DAOPBQ010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62733_length_1579_cov_3.888451"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1454
                     /locus_tag="PKW60_15065"
     CDS             90..1454
                     /locus_tag="PKW60_15065"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK00805.1"
                     /translation="MQTILAIDDERSVRTSYDAILAGFYRVIHAEDGASALRLLETSH
                     VDLAILDLMMPGLSGMDVLEQFEARGWDFPVIVVTAHKSVGMAVNAMKHGAFDYILKP
                     FDVDEILLTVERALTQHRHTQALTVLREADTLKFDAIIGESPALTRVLELARKAMRVD
                     STVLLSGESGTGKDLLARCIHSGGPRQAEAFVPVSCCAIPEHLVESELFGHERGAFTG
                     AVEKRVGKIQVADKGTLFLDEIGEMPLDAQAKLLRVLQDGQFYPVGSSKAIESDVRFI
                     CATNRDLRERIQEGAFREDLFYRVNVLPIVMPPLRQRREDIPLLTSHFLAKHAPRVNA
                     HATRFTPDALAMLAAHEWPGNVRELENIVERVLVHHADVSEIGVEHLESLVQMRAGTP
                     AETWAEFEGLPMEEATARLERHLILRALERAEYVQSRAADLLGTTRRILKYKMDQLEI
                     SPPS"
BASE COUNT          332 a          475 c          483 g          289 t
ORIGIN      
        1 cagacagcac cggggtgaac tgaagctcct gtcctgcgaa gagggcggca gcgccttcgc
       61 cttgagtgtg ccatcgggtc aagacggcca tgcagaccat cctggcgatt gatgatgaac
      121 gcagcgtccg cacttcctac gacgcgatcc tggcgggctt ctatcgcgta atccacgccg
      181 aagacggcgc atccgcgtta cggctccttg agacaagcca cgtagacctt gcgattctcg
      241 atttgatgat gccgggcctg tcgggcatgg acgtgcttga acagttcgaa gcccgcggct
      301 gggacttccc cgtcatcgtc gtcacggccc acaaaagcgt cggcatggct gtgaacgcca
      361 tgaaacacgg cgccttcgat tatattctga aaccattcga tgtggacgag attctcctga
      421 ccgttgagcg cgcgctcacc cagcaccgcc acacgcaggc gctgactgtg ctccgggagg
      481 cggacacatt gaagtttgac gccatcatcg gcgagagtcc tgccctcacg cgcgttctgg
      541 agctggcccg caaagccatg cgggtggatt caacagtact tctctccggc gaaagcggca
      601 ccggtaagga cctgctggcg agatgcatcc attcgggcgg gccgcgccag gccgaagcct
      661 tcgtgcccgt gtcctgttgc gccatacccg agcatctggt cgaaagcgaa ctctttggac
      721 atgagcgggg ggcgtttaca ggcgccgttg agaagcgcgt gggaaagatc caggtggctg
      781 acaaaggcac cctgttcctc gacgaaatcg gcgagatgcc cctggatgcg caggcaaagc
      841 tcctgcgggt gttgcaggac ggccaattct atcctgtagg cagcagcaaa gccatcgaat
      901 cggacgtgcg gttcatctgc gcgaccaacc gggacctccg ggaacgcatc caggagggcg
      961 ccttccgtga ggacctgttc taccgggtca atgttcttcc gatcgtcatg ccgccgctgc
     1021 ggcaacgccg ggaagacatc cccctgctga cgtcgcattt tctggccaaa cacgcgcccc
     1081 gggtgaatgc acacgccacg cgtttcacgc ccgacgccct cgcgatgctc gccgcgcacg
     1141 agtggcccgg gaatgtgcgt gaactggaga acatcgtgga gcgcgtcctg gttcaccatg
     1201 cggacgtttc cgaaattggt gtggagcacc tggaaagcct ggtgcagatg cgggcaggaa
     1261 caccggccga aacctgggcg gagtttgaag gactgcccat ggaggaggct acagcgcggc
     1321 tcgagcggca tctgatcctt cgggcactgg agcgcgcgga gtacgtgcag tcgcgcgcag
     1381 cggacttgct gggaacaacg cggaggatcc tcaaatacaa aatggaccaa ctggaaatat
     1441 cccccccttc ctgaaggccc gagaacgtga aatcacaaaa aagcacggaa attgcacatg
     1501 ccggcgtatc gtattaaaat aaccccttgg tatagtgaac gaatttgtct ggacgcggcc
     1561 gaagcgcccg attctctaa
//
LOCUS       DAOPBQ010000643         1570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_63252_length_1570_cov_3.851485, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000643 DAOPBQ010000000
VERSION     DAOPBQ010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63252_length_1570_cov_3.851485"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PKW60_15070"
     CDS             complement(<1..357)
                     /locus_tag="PKW60_15070"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPK00806.1"
                     /translation="MSAMETPSIWNVSQLTRRVKELLEREIGQVWLTGEISNFRVSPA
                     GHAYFTLKDEQSQIDAVMFRGRMARLQFTPDSGLQVIVAGQVTVYERRGSYQIVCDEM
                     HPKGVGALQLAFEKLKK"
     gene            519..>1570
                     /locus_tag="PKW60_15075"
     CDS             519..>1570
                     /locus_tag="PKW60_15075"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00807.1"
                     /translation="MRFAQVFGTARALALCFLAAWPAAAMEVAWWVSSEDMLNTLTAQ
                     PGLALGPEAPGADLRIDDTQRYQTILGLGASLDHATCYNISQLPPGEQERAVEWIVSP
                     YTGIGMNLMRICIGTPDFTPSPWYTYADIPGDLSLEHFSIEKDREYVLPILKMALEIN
                     PDLLFFASPWSPPGWMTSNGHIGGGRFDSAYYAPYARYLAKFIKAYEAEGIPIYAITL
                     QNEPDYNPPTYPTCRWTFKQQAEFIASHVGPEFERQGIQTRLWCWDHNFEPIGWPRNV
                     LNDPMAAPYIDGTAFHHYAGRPSSMARLSAMFPSKHIYFTEGSTSGVRGAIEIIAILR
                     NWARSYNAWVTVLDDKG"
BASE COUNT          309 a          508 c          456 g          297 t
ORIGIN      
        1 tttcttgagc ttctcgaagg cgagttggag cgcccccacg cctttcgggt gcatctcatc
       61 gcagacgatc tgatacgagc cgcggcgttc gtataccgtc acctgcccgg ccactatcac
      121 ctggagcccg ctgtcgggcg tgaactgcag ccgggccatg cggcctcgaa acatcaccgc
      181 gtcaatctgg ctctgttcgt ccttcagcgt gaaataggca tgacccgcgg gcgacacgcg
      241 aaaattcgaa atttcgcccg tcaaccacac ctgaccgatc tcgcgttcca ggagctcttt
      301 tacgcgacgc gtcagctggc tcacattcca gatgctgggt gtttccatag cggacatggg
      361 cacgattgta ctcgatcttc ctatgcccgg aaaaccggcc cgttccggag ggcgtatcgc
      421 gcatcattta cgcggaggcc gcacaatggt agtctattca ccaagagccg gcggaaaccg
      481 cccccgtttc ggacggcatg gagaaaggac ggcacgcgat gcgattcgca caggtgttcg
      541 ggacggcacg cgccctggcg ctgtgtttcc tggccgcatg gccggcggcg gccatggagg
      601 tggcgtggtg ggtctcctcc gaagacatgc tgaacacgct gacggcccag ccgggcttgg
      661 cgctgggccc ggaggcgccc ggcgcggacc ttcggataga tgacacgcaa cggtatcaga
      721 ccatcctggg cctcggcgcc tccctggacc acgcgacatg ctacaacatc agccagcttc
      781 cgcccggaga acaggaacgg gccgtcgagt ggatcgtatc tccctatacg ggaattggaa
      841 tgaacctgat gcgcatctgc atcgggacgc ccgatttcac cccgtctccg tggtatacct
      901 acgcggacat tccgggcgac ctgtcactcg agcacttctc cattgaaaaa gaccgcgaat
      961 acgtgctccc gatcctcaaa atggcgttgg agatcaatcc ggatctgctg tttttcgcgt
     1021 cgccgtggag cccgcccggc tggatgacgt cgaacgggca catcggcggc ggacgctttg
     1081 attccgcata ctacgcgccg tatgcacggt acctggcgaa attcatcaag gcgtatgagg
     1141 ccgagggcat ccccatttac gcgatcaccc tgcagaacga gcctgactac aatccgccga
     1201 cctatcccac gtgccggtgg acgttcaagc agcaggccga attcatcgcc agtcacgtgg
     1261 gcccggaatt cgagcgccag ggcattcaga cccgcctctg gtgctgggat cataatttcg
     1321 agcccatcgg atggccgaga aacgtgctga acgacccaat ggccgcgccg tatatcgacg
     1381 gcaccgcctt ccatcactac gcggggcgcc catcaagcat ggcgcgcctg agcgccatgt
     1441 tcccgtcaaa acacatctat ttcacggaag ggtccacttc cggtgtgcgg ggcgccatcg
     1501 agatcatcgc catcctgcgc aactgggccc gcagctacaa cgcctgggtc accgtgctcg
     1561 acgataaagg
//
LOCUS       DAOPBQ010000644         1567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_63422_length_1567_cov_3.080688, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000644 DAOPBQ010000000
VERSION     DAOPBQ010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63422_length_1567_cov_3.080688"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            434..1144
                     /locus_tag="PKW60_15080"
     CDS             434..1144
                     /locus_tag="PKW60_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPK00808.1"
                     /translation="MNLMHITDQLSAEIADLQFAPPVTCVYNPLEYARAGWDAYVKAY
                     GQGKREILLVGMNPGPFGMAQTGVPFGDVCSVRDWMGIEAAIHKPMVEHPRRPVEGFA
                     CRRAEVSGQRLWGWAAERFGSAERFFSRFFIANYCPLCFLEASGRNRTPDKLRADERA
                     LLFAACDRALRAQIGYLQPQYVLGIGQFAESRVLGVVNDDRIRTGRILHPSPASPAAN
                     RGWSQAVEQTLRKMGIAF"
     gene            complement(1141..>1567)
                     /locus_tag="PKW60_15085"
     CDS             complement(1141..>1567)
                     /locus_tag="PKW60_15085"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HPK00809.1"
                     /translation="GGMGSIAFACADLAGEVHSVENVPEASADGRHNARANGIDNVVF
                     HTMKVEDYLHGLAERQLTFAEDSAVVVDPPRAGMHPRALRCLLELAPRRFLYVSCKPT
                     VLAQEMASFLEAYRIADMRAVDMFPHTPHVELVVQFERR"
BASE COUNT          274 a          489 c          474 g          330 t
ORIGIN      
        1 gcgtgaccag tccggttcca gagcgtgcgg cgcccagccc cgccagttgc acggcgcccg
       61 tataacccac ggcgcctccg attataagca catgtccgaa ggtccccttg tggccgccct
      121 ggggccgctc gggcagcttt tccgcggcca gtgccttcgt gatctcgaca agcatgatcc
      181 gttccccacc gatttccctc attgtgcgca tgcctgggag gcattgcgcg gcgattctat
      241 cacgttgcag gcgcaaatta accttttgat tcgcggcgtt cgaatgccga agccccggca
      301 ttcgcaggtc cctcgggttt caatcggcgg cacaatgcct tcagtttcga gtgtcatgta
      361 cgattatctt tcaacagcga ttcctttccg ccacgcctgt ccgggcgcgt ctgtcttttg
      421 ctatactccg cccatgaatt tgatgcatat cactgaccag ctcagcgcgg aaatagcgga
      481 tttgcagttt gcaccgccgg ttacctgtgt ctacaacccg ctggaatatg cccgggcagg
      541 ctgggacgcc tatgtgaagg cgtacggcca gggcaaacgt gagattctgc tcgtcggcat
      601 gaatcccggc ccgtttggca tggcccagac gggtgtgccg ttcggcgatg tatgctccgt
      661 tcgtgactgg atgggcattg aggctgcaat ccataaaccg atggtcgagc atccgcgccg
      721 cccggtcgaa ggtttcgcgt gccggcgggc tgaagtgagc gggcagcgtc tctggggctg
      781 ggccgcggaa cgcttcggca gcgcggaacg ctttttttca cgctttttta tcgccaatta
      841 ctgtcccttg tgctttttgg aagcttccgg ccggaaccgc actcccgaca aattgcgtgc
      901 ggatgaacgt gcgcttctct tcgcggcgtg cgatcgggcg ctgcgcgccc agatcggcta
      961 ccttcagccc cagtatgtgc ttgggatagg ccagtttgcg gaaagccggg ttctgggggt
     1021 ggtgaatgat gaccgcattc ggacaggccg gattcttcat ccgagcccgg ccagtcccgc
     1081 agctaaccgg ggttggagcc aggcggtcga gcagacgctg cgcaagatgg gcatcgcgtt
     1141 ttagcgccgc tcaaattgaa ccaccagctc gacgtgtggc gtgtggggaa acatatcaac
     1201 ggcgcgcata tccgcaattc ggtacgcctc gagaaacgag gccatctcct gcgccagcac
     1261 cgtgggtttg cacgagacgt agaggaatcg ccgcggcgcc agctcgagca ggcaacgcag
     1321 cgccctgggg tgcattcccg cccgcggcgg atcgaccacc acggccgaat cctcggcaaa
     1381 ggtcagctgc ctctcagcca atccatgaag atagtcttcg accttcatcg tatggaacac
     1441 gacattgtca atgccgtttg cccgcgcgtt gtgccggccg tcagccgacg cctcggggac
     1501 gttctccacg gagtgcactt cgccggcgag gtccgcacag gcgaaggcga tcgaccccat
     1561 accgccg
//
LOCUS       DAOPBQ010000645         1538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_65154_length_1538_cov_4.438301, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000645 DAOPBQ010000000
VERSION     DAOPBQ010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65154_length_1538_cov_4.438301"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKW60_15090"
     CDS             <1..396
                     /locus_tag="PKW60_15090"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK00810.1"
                     /translation="RYALPWHKKLAQRTHKAGVPYFLHSCGNLDAIMDNLIDDAGIDG
                     KHSFEDAIIPVEDFQARYGDRIAVLGGVDINRLSAGTPGEVRAHTRYLIETCGPRGRY
                     AIGSGNSIPSYVPAENYLAMLDEAHDVNG"
     gene            491..>1538
                     /locus_tag="PKW60_15095"
     CDS             491..>1538
                     /locus_tag="PKW60_15095"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HPK00811.1"
                     /translation="MHSVPIGLLCAIISATRIWTASSLDNIYPDSQPPPGAAPHVRLY
                     AAQGEYESFQICIRSSRRDIENVEVVPEPLAKAIDSPEIRNVGYLAVPEPSPRACGGQ
                     TAQPDPLHPFEPFFLEKDTTAALWVTYTIPRDCRAGIYQGRLGLRLAGRARQYVRVTI
                     EVFDFQIPEFPSITVTGRLSAERIRTFNEVADDTLGEWRPFYDALAPWRIAFNTALEF
                     PLAPGDSEPYLEHLLYAARRAHAPVIFAGMGGRLADALARESSLDTLQAVHEALAGTD
                     WSRRICAEPMPMLPRSHWVEVRDRYEPFFEQAPSIRRLFQGVPHPQTEQLAEIWVMPL
                     QFFDPIAVQRLRTGM"
BASE COUNT          289 a          466 c          452 g          331 t
ORIGIN      
        1 cggtatgcac tgccctggca taaaaagctg gctcagcgga cgcacaaggc cggcgtgcct
       61 tactttctgc attcctgcgg caacctcgac gccattatgg acaatttaat tgacgacgca
      121 ggcattgacg gaaagcattc gttcgaagat gccattatcc cggtcgagga ttttcaggcc
      181 cgctatggcg acaggatcgc cgtgctgggc ggagtcgata ttaaccgcct gagcgccggg
      241 acgcccgggg aagtccgggc ccacacgcgg tatctgatcg agacttgtgg cccgcgcggc
      301 cgttacgcca tcgggtccgg caattctatc cccagctatg tgcctgcgga gaattatctg
      361 gccatgctcg atgaggcgca cgacgtgaac ggatgaggtc ctcctgcccg ggtggcgtca
      421 acctgcccgc tgtggggttc gttttcatcg aaagtatacg cttgtttgca atgacaagag
      481 gtcgccgcac atgcacagcg tgcccatagg cctcctgtgc gcgatcattt ccgctacgcg
      541 catctggacg gcatccagcc tggacaacat ctacccggac agccagccgc cgcctggggc
      601 cgcgccgcat gtccgtcttt acgccgcaca aggggaatat gagtcgtttc agatctgtat
      661 ccgttcttcg cgccgtgaca tcgaaaatgt tgaagtggtg cccgaacctc tcgcaaaggc
      721 catcgattct cctgagatac ggaacgttgg ctatctggcc gttccagagc catccccgcg
      781 tgcctgcggc ggacaaaccg cccagccgga cccgcttcat ccatttgagc ctttctttct
      841 cgaaaaagac accaccgcgg cgctatgggt gacgtatacg atccctcgag attgccgtgc
      901 ggggatctac cagggccggc taggattgcg cctggctggg cgcgccagac agtatgtccg
      961 cgtcacgatt gaagtctttg attttcagat tcccgaattt cccagcatca ctgtgacagg
     1021 gcgcctctct gcagaacgta ttcgcacgtt taacgaggtc gccgatgaca cgctcggcga
     1081 gtggcgcccg ttttacgatg cactcgcacc ctggcgaatc gctttcaata ccgctcttga
     1141 gttcccgctt gcccccggcg acagcgagcc atacctggag catttgctgt acgctgcccg
     1201 ccgcgctcat gcgccggtaa tcttcgcggg aatggggggg aggctggcgg atgccctggc
     1261 gcgtgaatcg tcgttggaca ccttgcaggc cgttcatgaa gccttggcgg gcacggactg
     1321 gtcgaggcgc atatgcgcgg aaccgatgcc gatgctgccc cgatcgcatt gggtggaggt
     1381 gcgtgaccgg tatgagccat ttttcgaaca ggcgccgtcc atacgccggt tgtttcaagg
     1441 agtgccccat cctcaaacag agcagcttgc cgaaatctgg gtgatgccgc ttcaattttt
     1501 cgatccgatt gcggtacagc ggctccgaac aggcatgt
//
LOCUS       DAOPBQ010000646         1518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_66456_length_1518_cov_2.652085, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000646 DAOPBQ010000000
VERSION     DAOPBQ010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66456_length_1518_cov_2.652085"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PKW60_15100"
     CDS             complement(<1..1010)
                     /locus_tag="PKW60_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00812.1"
                     /translation="MEQQTLRGYLENSADLPPLPELTAGPVSALLESPHGREILKWVQ
                     GAGREIPETTQALYDRYKEAGERFPYETPYFDKRELVTQHALANWLGVDGADLSHLCA
                     LLESVLAEPTWVLPAHVRPFPWNVDLFSAETACDLAHLLFILEDRLPPALAGRMRAEI
                     RARVMDPYLEHAHDYWWGSGRNNWTGVCAGAIGETFLLLEPDAERQAQAVNLVLDQMD
                     RFFQHAFEEDGACLEGIDYWNYGLLHCVAFGEMLRVRTNGAVDLLAHPKLKAIATYPA
                     AAALGRHVFASFADSYEQSTIVPFIAARLAQRTGVESLLLQAGGMTEWRLMTVLRNLS
                     WW"
BASE COUNT          319 a          433 c          465 g          301 t
ORIGIN      
        1 tgccaccagg agaggttgcg cagcacggtc atcagccgcc attccgtcat accgccggcc
       61 tggagcagca gcgattcaac gccggtgcgc tgggcgagcc gggcggcgat aaacgggact
      121 atggtggatt gttcatagct gtccgcaaac gaggcaaaaa cgtgccggcc caacgcagcg
      181 gcggcgggat aggtggcgat agccttgagt ttagggtgtg ccaacaggtc cacagcaccg
      241 ttcgtcctga cgcggagcat ttcgccaaag gccacgcaat gaagcagccc gtaattccag
      301 taatctatgc cttcaaggca tgcgccgtct tcctcaaacg cgtgctgaaa aaagcggtcc
      361 atctgatcga gcacgaggtt cacggcctgt gcctggcgct cggcatcggg ctccagcagc
      421 aaaaacgtct cgccgatggc cccggcacag acaccggtcc aattgttgcg gccgctgccc
      481 caccagtagt cgtgggcatg ctcgagatag gggtccatca cccgggctcg aatctcggcg
      541 cgcatgcgcc ctgcgagcgc cggcggcaac cgatcctcca gaatgaagag caggtgcgcg
      601 aggtcgcagg ccgtttccgc tgagaagagg tcgacattcc acggaaaggg gcgcacgtgt
      661 gcgggcagca cccaggtggg ttcggcgagc acgctttcaa gcagcgcgca caagtggctc
      721 aggtcggcgc cgtccacgcc cagccaattc gccaatgcgt gttgcgtcac cagttcgcgc
      781 ttgtcgaaat acggcgtttc atacgggaat cgttccccgg cctctttata ccgatcgtac
      841 agcgcctggg tggtctctgg aatttcgcgg cctgccccct gcacccattt gaggatttcg
      901 cggccatgcg ggctttccag cagggcgctc acagggcccg cggtaagctc aggcaagggt
      961 ggaagatcgg cggaattctc gagatatccg cgcaaggttt gctgttccac tggagtcgcc
     1021 agtgtttccc cggacgctcc agcggctgtg taacccgcca gaagcagaat cgaaagggct
     1081 gttatggtgc gcatcatggc cgacagcata acgaatcgaa cccattgtgt ccagaaaaag
     1141 gcgcccgaag cgcgaaaact ccggacgaat ggcaaagtat gggcggcagg tctggtacgg
     1201 gcgcaaacac ggagatccgg gctcctggaa cggcttgcat ttttgctctc gcggccactg
     1261 ggaaacgggt gctggtgacc acaattagtg gcctttttct cattgaaacg ggccccgaac
     1321 aacctggtaa actctaagta ttgtggtttt cgtttcctct tgcatacata tagtgttcat
     1381 gatagtatct aaccgctata ttgtgtagtg ctctgcatcg gggagggatt ttcgcaacat
     1441 gttgtggtgt cccgcgggca cgacacgaaa aaatgcctga aggagagggt atgaaatcgc
     1501 acagtatttt ggtccggt
//
LOCUS       DAOPBQ010000647         1514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_3481 NODE_66701_length_1514_cov_3.663468, whole genome
            shotgun sequence.
ACCESSION   DAOPBQ010000647 DAOPBQ010000000
VERSION     DAOPBQ010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865216
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 19:57:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,021
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,975
            CDSs (with protein)               :: 2,975
            Genes (RNA)                       :: 28
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 0 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66701_length_1514_cov_3.663468"
                     /isolate="AMR_MDS_3481"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="PKW60_15105"
     CDS             complement(<1..1400)
                     /locus_tag="PKW60_15105"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPK00813.1"
                     /translation="MQETDLSTLTWQLYGWRPHTWRLGKSMETGAAMQADIGPVPARV
                     PGSVQQTLLEAGILPDWNVGLNSLACEWVELRHWDFSAILPAGLITPGQRVFLDAQGL
                     DYSGWILVDCREVAVFKGTLTPHLLELTPALCDGEAHQLSIVFDVPPPEQGQIGFTSH
                     SRCFKPRFCYSWDWTPRLVPIGVWDTLSLKSAAQCAIDPIHTRALLAEDHRTGRVEVA
                     LQADALIVPPAEIGEVTVTLLNGDAALARSSGRFTRERLDIVLEGIAVEAWWPNGFGP
                     AKTYTLRLESVIHGEMWRLERVVGFKRVRWLPCEGAPDDAEPWICEVNGRPLFLQGAN
                     WVPPRTSYPDSSDEEYRRLIELYRDMGCTCLRVWGGAILEKEIFYRLCDEAGILVWQE
                     FPLSSSGVENWPPEDPETIVELSHICMSYIRRRAHHPALLLWCGGNELFGGGGKGVDN
                     VPCDYTHPCLAAMKEVV"
BASE COUNT          310 a          466 c          476 g          262 t
ORIGIN      
        1 accacctcct tcatcgcggc gaggcaggga tgggtataat cgcacgggac gttgtccacg
       61 cctttgcctc caccgccgaa gagttcgttg ccaccgcacc acaagaggag cgcgggatgg
      121 tgcgcgcgcc ggcggatata gctcatgcag atgtgcgaca actccacgat cgtctcaggg
      181 tcctcgggcg gccagttttc cacccccgaa ctcgacaacg gaaactcctg ccagaccagg
      241 atgcccgctt cgtcgcagag gcggtagaag atctcttttt cgaggatggc cccaccccag
      301 acgcggaggc aggtgcagcc catgtcgcgg tagagttcaa taagccggcg gtactcctcg
      361 tcgctgctgt cgggatagct ggtacgcggc gggacccagt tggcgccctg aagaaagagg
      421 ggcctgccat tcacttcgca gatccagggt tcagcatcgt cgggcgcgcc ttcacaggga
      481 agccaccgga cgcgtttgaa ccccacgacg cgctcaagcc gccacatctc gccgtgaatg
      541 accgattcca gccggagcgt gtacgttttc gcggggccaa agccgttggg ccaccacgct
      601 tcgacagcaa tgccttccag gacgatgtcc aggcgttcgc gcgtaaaacg gcccgaactg
      661 cgggcgagcg cggcatcgcc gttgaggagg gtcacggtga cctcgccaat ctcagccggc
      721 ggcacaatga gtgcatcggc ctgcaatgcc acttcgacgc ggcccgtccg gtggtcctcg
      781 gcgagcaacg cgcgggtatg aatgggatca atcgcgcatt gcgcggcact cttgaggctg
      841 agcgtgtccc agacgccgat cgggacgagc cggggcgtcc agtcccagct gtaacaaaaa
      901 cggggtttga aacagcgcga atgcgaggta aagccgatct ggccctgctc aggcggcgga
      961 acatcgaaaa cgatactcag ctgatgcgct tcgccatcgc acaaagccgg agtcagttcc
     1021 agcaggtgcg gagtcaatgt gcctttaaac accgcaactt cgcggcaatc gacgagaatc
     1081 cagccggaat aatcgagtcc ctgggcatcg aggaagacgc gctggccggg ggtgatcaat
     1141 ccggcgggaa ggatggccga gaagtcccaa tggcggagtt caacccattc gcaggcgagc
     1201 gaattcaggc caacgttcca gtccggcagg atgcccgctt cgagcagcgt ctgctgaacc
     1261 gaccccggca cacgggcagg aaccgggccg atatcggcct gcatggccgc ccccgtttcc
     1321 atggacttcc ccagccgcca cgtgtgcggc cgccaaccgt aaagttgcca ggtcaatgtg
     1381 ctgagatcgg tttcctgcat gctgtccgcc ctctggtgaa gatgagatgg ttgttgaatt
     1441 attggccggt attgtgggct gccaatcgga caatatcaac tggaggagcg tgccacaggc
     1501 cgtgagagtc gatc
//
